LOCUS       DAJVUJ010000001       107626 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.001, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000001 DAJVUJ010000000
VERSION     DAJVUJ010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 107626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 107626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..107626
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.001"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(1..610)
                     /gene="yeeE"
                     /locus_tag="OQQ48_000001"
                     /pseudo
     CDS             complement(1..610)
                     /gene="yeeE"
                     /locus_tag="OQQ48_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
     gene            complement(789..2147)
                     /gene="plaP"
                     /locus_tag="OQQ48_000002"
     CDS             complement(789..2147)
                     /gene="plaP"
                     /locus_tag="OQQ48_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HCS1950915.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(2137..2199)
                     /gene="yoeI"
                     /locus_tag="OQQ48_000003"
     CDS             complement(2137..2199)
                     /gene="yoeI"
                     /locus_tag="OQQ48_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HCS1950916.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(2414..3343)
                     /locus_tag="OQQ48_000004"
     CDS             complement(2414..3343)
                     /locus_tag="OQQ48_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1950917.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLALEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQHFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(3389..4213)
                     /locus_tag="OQQ48_000005"
     CDS             complement(3389..4213)
                     /locus_tag="OQQ48_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCS1950918.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGSGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGMVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(4296..4550)
                     /gene="yoeB"
                     /locus_tag="OQQ48_000006"
                     /pseudo
     CDS             complement(4296..4550)
                     /gene="yoeB"
                     /locus_tag="OQQ48_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
     gene            complement(4547..4798)
                     /gene="yefM"
                     /locus_tag="OQQ48_000007"
     CDS             complement(4547..4798)
                     /gene="yefM"
                     /locus_tag="OQQ48_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HCS1950919.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            5081..5131
                     /gene="hisL"
                     /locus_tag="OQQ48_000008"
     CDS             5081..5131
                     /gene="hisL"
                     /locus_tag="OQQ48_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HCS1950920.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    5107..5230
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            5277..6176
                     /gene="hisG"
                     /locus_tag="OQQ48_000009"
     CDS             5277..6176
                     /gene="hisG"
                     /locus_tag="OQQ48_000009"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HCS1950921.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            6182..7486
                     /gene="hisD"
                     /locus_tag="OQQ48_000010"
     CDS             6182..7486
                     /gene="hisD"
                     /locus_tag="OQQ48_000010"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HCS1950922.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDEALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPAAD
                     MARRVAEAVERQLAELPRAETARQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARDLVDGITSAGSVFLGDWSLESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            7483..8553
                     /gene="hisC"
                     /locus_tag="OQQ48_000011"
     CDS             7483..8553
                     /gene="hisC"
                     /locus_tag="OQQ48_000011"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HCS1950923.1"
                     /translation="MSIVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYGVSAETIGVECRTVPTLDNWQLDLQGISDKLEGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVAQIIAER
                     ECLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKHPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            8553..9620
                     /gene="hisB"
                     /locus_tag="OQQ48_000012"
     CDS             8553..9620
                     /gene="hisB"
                     /locus_tag="OQQ48_000012"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HCS1950924.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFLQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            9620..10210
                     /gene="hisH"
                     /locus_tag="OQQ48_000013"
     CDS             9620..10210
                     /gene="hisH"
                     /locus_tag="OQQ48_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HCS1950925.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            10210..10947
                     /gene="hisA"
                     /locus_tag="OQQ48_000014"
     CDS             10210..10947
                     /gene="hisA"
                     /locus_tag="OQQ48_000014"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HCS1950926.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPEMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            10929..11705
                     /gene="hisF"
                     /locus_tag="OQQ48_000015"
     CDS             10929..11705
                     /gene="hisF"
                     /locus_tag="OQQ48_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HCS1950927.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDGETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            11699..12310
                     /locus_tag="OQQ48_000016"
     CDS             11699..12310
                     /locus_tag="OQQ48_000016"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HCS1950928.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(12407..13384)
                     /gene="wzzB"
                     /locus_tag="OQQ48_000017"
     CDS             complement(12407..13384)
                     /gene="wzzB"
                     /locus_tag="OQQ48_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HCS1950929.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDFLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIVTQPDVGQIAGYTNAMNVIYGPAVPKVSDIQTSLIGRYSTA
                     FSALAETLDNQEEPEKLTIEPTVKNQSLPLAVSYVGETAEGAQKQLAQYIQQVDDQVN
                     EELEKDLKDNIALRMKNLQDSLKTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSSNYYQTRQNLLDIESLKVDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(13531..13623)
                     /locus_tag="OQQ48_000018"
                     /pseudo
     CDS             complement(13531..13623)
                     /locus_tag="OQQ48_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356562.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
     gene            13793..14797
                     /locus_tag="OQQ48_000019"
     CDS             13793..14797
                     /locus_tag="OQQ48_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010432020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HCS1950930.1"
                     /translation="MKFLVTGAAGFIGFYVSKRLLAAGHQVVGIDNLNDYYDVNLKQA
                     RLDLLKHDNFSFYKIDLADREKMAALFADERFDRVINLAAQAGVRYSLENPNAYADAN
                     LIGFLNILEGCRHNNVQHLLYASSSSVYGMNRKMPFSTDDSVDHPVSLYAATKKANEL
                     MAHTYSHLYGLPTTGLRFFTVYGPWGRPDMALFKFTKAMLEGKSIDVYNFGKMKRDFT
                     YIDDIAEAIIRLQDVIPEKDPQWTVETGSPATSSAPYRVYNIGNSSPVELMDYINALE
                     EALGIEANKNMMPLQPGDVLETSADTKALYDVIGFKPETSVKEGVKNFVEWYRNFYKV
                     "
     gene            complement(14863..16029)
                     /gene="ugd"
                     /locus_tag="OQQ48_000020"
     CDS             complement(14863..16029)
                     /gene="ugd"
                     /locus_tag="OQQ48_000020"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HCS1950931.1"
                     /translation="MKITISGTGYVGLSNGILIAQNHEVVALDIVQAKVDMLNKKQSP
                     IVDKEIEEYLATKDLNFRATTDKYDAYKNADYVIIATPTDYDPKTNYFNTSSVEAVIR
                     DVTEINPNAVMIIKSTIPVGFTESIKERFGIENVIFSPEFLREGKALYDNLHPSRIVI
                     GEQSDRAERFAALLQEGAIKQDIPILFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNSRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADSILARKPKVVGVYRLIMKSGSDNFRASSIQGIMKRIKAKGVPVIV
                     YEPAMKEDDFFRSRVVRDLDAFKQEADVIISNRMSADLADVADKVYTRDLFGND"
     gene            complement(16278..17684)
                     /gene="gndA"
                     /locus_tag="OQQ48_000021"
     CDS             complement(16278..17684)
                     /gene="gndA"
                     /locus_tag="OQQ48_000021"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCS1950932.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGNKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKDGVFHTEWLD"
     gene            complement(17753..18859)
                     /gene="glf"
                     /locus_tag="OQQ48_000022"
     CDS             complement(17753..18859)
                     /gene="glf"
                     /locus_tag="OQQ48_000022"
                     /EC_number="5.4.99.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HCS1950933.1"
                     /translation="MQYEFIIIGSGLFGAVCAYELNKRNKRVLVIEKRDHIGGNIYTE
                     KVDDIHIHKYGAHIFHTNDQYIWNYINKFASFNRFTNSPLAYHKGKLYNLPFNMNTFY
                     QLWGTKTPSEAQDKIDLQRSKYSNITPTNLEEQALSLVGEDVYEKLIKGYTEKQWGRS
                     CSELPPFIIKRIPVRFTFDNNYFTDRFQGVPIGGYTQIIDKMLNGVDLLLNVDYLSEK
                     DKYNSMAERIIYTGPIDAYFDKKLGTLEYRSLKFITKKIEEANYQGNAVINYTEKDIP
                     FTRIIEHKHFDDSYETEYTFITEEYPIEWSEGQEPYYPINDKKNMDIYHKYRELSQME
                     SKVIFGGRLAEYKYYDMHQVIRSALNCVNKILHD"
     gene            complement(18892..19422)
                     /gene="rfbC"
                     /locus_tag="OQQ48_000023"
     CDS             complement(18892..19422)
                     /gene="rfbC"
                     /locus_tag="OQQ48_000023"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011093025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HCS1950934.1"
                     /translation="MKVQDTVLDGVKIIEPVVYGDNRGFFLEVYQKQRYQNLLNIEFD
                     FVQDNYSRSSKNVLRGLHFQKTKPQGKLVRVVRGEVFDVAVDIRKESPNYGQWIGVLL
                     SEMNKKQFWVPPGFAHGFVVLSEMADFEYKCTEYYDPEDECCLLWNDPELNIQWPLSN
                     PILSDKDMKGCLLKEL"
     gene            complement(19437..20606)
                     /locus_tag="OQQ48_000024"
     CDS             complement(19437..20606)
                     /locus_tag="OQQ48_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCS1950935.1"
                     /translation="MDCLDEKDHLRDNHSLKIVLTANSSWYIYNFRRSTIKALINIGF
                     DVLVVVPDYEYKDSILKFGAKFEKVDLKPKSINIFTECTSLISYYKIIKKFNPQVILT
                     FTPKANIYCGLLAYKFDAKVIPNISGLGSAFVNSNTILSQIVKLLYKIALKKAAFVFF
                     QNEDDRQLLINSGCVNYEKTCRIFGSGVDLSKFLPSNKNLKQRSCVKFILVARLLYTK
                     GILHYLKAAEIIKSKYPNCSFSLLGAFEKNEKIITKELIEGFCTRGIVNYYGTSDDVA
                     SIMKHYDAIVLPSFYREGVPKALIEGASSGLAILTTDNVGCRDMVQNEVNGFLCQPND
                     LDSLVDIIEKYILLTDEMKNTMSIKSREFAESHCDENKIIEKYTNTINDVLNRNH"
     gene            complement(20569..21441)
                     /locus_tag="OQQ48_000025"
     CDS             complement(20569..21441)
                     /locus_tag="OQQ48_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCS1950936.1"
                     /translation="MNRVAVIIVLYYPDVSKINKMIDSLGKKQCEILLVDNTPELHLE
                     SDVLCNNNIHYYHLGDNKGIAFAQNYGFIKAIGLGKSLFFTFDQDSCISKDYISSMLK
                     EYEYACSLRSNIAALGPTIINERNGKKYDREIKLGSQISETIHDVESIISSGALIPLE
                     AIINIGLNKVSWFIDLIDIEWSFRARKEGWNILVTDKVLISHNIGSKDINIMGIKTFT
                     VCSPFRLYYVYRNWLLALREPVFPLRYKLKKLITMPVRIIIYAFCERGGQRLNYIFKG
                     IKDGLLGRKGSFKG"
     gene            complement(22244..22876)
                     /locus_tag="OQQ48_000026"
     CDS             complement(22244..22876)
                     /locus_tag="OQQ48_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HCS1950937.1"
                     /translation="MLEHIVVFDVCGTLYLSNTTFDYVVFVHRKQKKYTKLIKCLLLR
                     SLFGKLLHKLRICSLRKHFLKTLEGYEKSTLEELANLFYDEFLIFKEKNEVMALLERY
                     KHEKIILMSASVDPVINVIAKNLNVEAVSSKLEFIDNKSTGLLEYNLTGIKHLQVNNP
                     VSLIVTDNFSDINLIKLADTAVIISKAKDKNKWRVLLNRFGVSKDKVRFM"
     gene            complement(22878..24059)
                     /locus_tag="OQQ48_000027"
     CDS             complement(22878..24059)
                     /locus_tag="OQQ48_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide repeat unit polymerase"
                     /protein_id="HCS1950938.1"
                     /translation="MTIFIAIIMLMYSGLLLSINKDIKSPSAILFFIWGGLLFLSGVN
                     GDITNYTLCIILFSCLSFSVGALLAKPYAPIFEHVFSYNINNTKALVLITNIISFVIL
                     IYTLIVFLYYYKGGFTESYINSRTEINYGDKSGLIKIYGYIYYLLYPLVYVWSFLYFK
                     NKYKKIEEGERARLPYNNRTFYFVFFTSLFYALLSTAKIKVLLLIIPIVFLRLFFSKI
                     SLKYLLVTVSGIIFFFYLSMLFLNKIDDSSGAFEALKYGLMNYSLANIFALDSVLQGK
                     AVVIDCNGDATCGLANFISYKEYKTNVFTIFYSLTKYSDFLYSLIFFFVIGFFHSSLY
                     NVAKKNKKTISVVICSILYFPLLFQFFDQLYMLMFYIYAIAMLYVICFCSRLTLTLKG
                     V"
     gene            complement(24062..24826)
                     /locus_tag="OQQ48_000028"
     CDS             complement(24062..24826)
                     /locus_tag="OQQ48_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4422 domain-containing protein"
                     /protein_id="HCS1950939.1"
                     /translation="MALFIACHKQTELPNHECYIPIHAGKKISGKMLDQVLGDDTGNN
                     ISCKNKNYCELTVIYWLWKNKLFNDDYIGLVHYRRYFGNVLSNFKFKEVRIYDRANIS
                     NKMKQYDIIIPVKESLKLSVVEHYRKFHNVEDLMLAKKIVDKLYPDYSVAFRELLEQK
                     KISLYNMFIMKAGIFDCYCDWLFSILDELEKEINLCRYDEYQSRVFGFIAERLLNVWI
                     NKNKEVFKVGEEFVINTDECSGRGAQLISQLRAKLK"
     gene            complement(24839..26086)
                     /locus_tag="OQQ48_000029"
     CDS             complement(24839..26086)
                     /locus_tag="OQQ48_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_050009647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HCS1950940.1"
                     /translation="MKLVTSLNKNIFYLAILQGSYFILPLLTFPYLVRVLGPYSFGLL
                     GFCQATMQYLVMVTDYGFNWSATQDVSKSKNDKEKLTTIFWNVFFSKIILALSAIVIF
                     FGITFFIKELYYAQVVLISFIPMVLGNILYPVWFFQGLEKMKWITLCSLSARLLLIPL
                     TFLLVKNPQDIWLAALIQGGVNFIAGLIGFAIIINNNWIGKLVFDYDGIIYSLKSGWH
                     IFISTAAISLYTTTTVVVLGFICGPVSVGYFNAANTIRNAAQSLMNPIYQAVYPRINS
                     LFEENYAKSMNLLKKSFKGVLFISFSGSLLLCILSPIIIRYGVGDKYTESISILRILA
                     FLPFIISLSNFFGIQTMLTHGYKRQFSKILLICGVMHVIIIAPLIFFAGAYGAAMTVL
                     ITETVVASLMYLFLKRKKINLFK"
     gene            complement(26129..27013)
                     /gene="rfbA"
                     /locus_tag="OQQ48_000030"
     CDS             complement(26129..27013)
                     /gene="rfbA"
                     /locus_tag="OQQ48_000030"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCS1950941.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPTPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDTAVDKESGATVFAYHVNDPERYGVVEFDKNGTAISL
                     EEKPLQPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDSEQVKILAEPL
                     KKNAYGQYLLNIINNKIS"
     gene            complement(27071..27970)
                     /gene="rfbD"
                     /locus_tag="OQQ48_000031"
     CDS             complement(27071..27970)
                     /gene="rfbD"
                     /locus_tag="OQQ48_000031"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HCS1950942.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPEFAQLLNATSVESIAKAANEVGAWVIHY
                     STDYVFPGTGEIPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALNKPEVAGMYHLVAS
                     GTTTWHDYAALVFEEARKAGIPLALNKLNAVPATAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            complement(27970..29055)
                     /gene="rfbB"
                     /locus_tag="OQQ48_000032"
     CDS             complement(27970..29055)
                     /gene="rfbB"
                     /locus_tag="OQQ48_000032"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCS1950943.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYVFEHADICDAAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDGDKKNSFRFHHISTDEVYGDLPHPDEVNNKEQLPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYIVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDAEKISRELGWKPQETFESGIRKTVEWY
                     LANAKWVENVKSGAYQSWIEQNYEGRQ"
     gene            complement(29427..30320)
                     /gene="galF"
                     /locus_tag="OQQ48_000033"
     CDS             complement(29427..30320)
                     /gene="galF"
                     /locus_tag="OQQ48_000033"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HCS1950944.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            complement(30495..31889)
                     /gene="wcaM"
                     /locus_tag="OQQ48_000034"
     CDS             complement(30495..31889)
                     /gene="wcaM"
                     /locus_tag="OQQ48_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HCS1950945.1"
                     /translation="MPFKKLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPGGLVCDNINTGIFIPAGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGEEGGSMHNITLDVRGSDCTIKGLTMSGFGPVTQIYIGGKNKQVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSCVALTNIEMKRASLELHNKPQHLFMRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERGE"
     gene            complement(31900..33120)
                     /gene="wcaL"
                     /locus_tag="OQQ48_000035"
     CDS             complement(31900..33120)
                     /gene="wcaL"
                     /locus_tag="OQQ48_000035"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HCS1950946.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTQN
                     THAAWTKYNLAARTRWLQDEPQGKVAKLRYRASQTLRGIHRKNTWQALNLKRYGAESR
                     NLILSAICGQVATPFHADVFIAHFGPAGVTAAKLRELDVIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISELWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELTTVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            complement(33117..34397)
                     /gene="wcaK"
                     /locus_tag="OQQ48_000036"
     CDS             complement(33117..34397)
                     /gene="wcaK"
                     /locus_tag="OQQ48_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HCS1950947.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHAEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVENGVDTAWLVDHHIEDF
                     TASYAVQHWLDVAAQQKAVAITLRELAPFDKRLGTTQEAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFSTPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPALNARLNEAVSRERQTGMRMVQSVLERIGEAK"
     gene            complement(34565..36043)
                     /gene="wzxC"
                     /locus_tag="OQQ48_000037"
     CDS             complement(34565..36043)
                     /gene="wzxC"
                     /locus_tag="OQQ48_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HCS1950948.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGIVVCVAVFLLSDAI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSVVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIIAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            complement(36045..37439)
                     /gene="wcaJ"
                     /locus_tag="OQQ48_000038"
     CDS             complement(36045..37439)
                     /gene="wcaJ"
                     /locus_tag="OQQ48_000038"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HCS1950949.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            complement(37494..38864)
                     /gene="cpsG"
                     /locus_tag="OQQ48_000039"
                     /gene_synonym="manB"
     CDS             complement(37494..38864)
                     /gene="cpsG"
                     /locus_tag="OQQ48_000039"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HCS1950950.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALARGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALSVDRTDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            complement(39057..40493)
                     /gene="cpsB"
                     /locus_tag="OQQ48_000040"
     CDS             complement(39057..40493)
                     /gene="cpsB"
                     /locus_tag="OQQ48_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HCS1950951.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEQAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(40496..41719)
                     /gene="wcaI"
                     /locus_tag="OQQ48_000041"
     CDS             complement(40496..41719)
                     /gene="wcaI"
                     /locus_tag="OQQ48_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HCS1950952.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADADV
                     DALRNQLDLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(41716..42195)
                     /gene="gmm"
                     /locus_tag="OQQ48_000042"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(41716..42195)
                     /gene="gmm"
                     /locus_tag="OQQ48_000042"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HCS1950953.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLMPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(42195..43163)
                     /gene="fcl"
                     /locus_tag="OQQ48_000043"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(42195..43163)
                     /gene="fcl"
                     /locus_tag="OQQ48_000043"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HCS1950954.1"
                     /translation="MSRQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRGE"
     gene            complement(43166..44287)
                     /gene="gmd"
                     /locus_tag="OQQ48_000044"
     CDS             complement(43166..44287)
                     /gene="gmd"
                     /locus_tag="OQQ48_000044"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HCS1950955.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(44314..44862)
                     /gene="wcaF"
                     /locus_tag="OQQ48_000045"
     CDS             complement(44314..44862)
                     /gene="wcaF"
                     /locus_tag="OQQ48_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HCS1950956.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKT
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(44878..45624)
                     /gene="wcaE"
                     /locus_tag="OQQ48_000046"
     CDS             complement(44878..45624)
                     /gene="wcaE"
                     /locus_tag="OQQ48_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HCS1950957.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQAEDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGITMAQGKFALFLNSGDIFHQDAANF
                     VRKLKMQKDNMMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKV"
     gene            complement(45635..46852)
                     /gene="wcaD"
                     /locus_tag="OQQ48_000047"
     CDS             complement(45635..46852)
                     /gene="wcaD"
                     /locus_tag="OQQ48_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HCS1950958.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAVVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            complement(46827..48044)
                     /gene="wcaC"
                     /locus_tag="OQQ48_000048"
     CDS             complement(46827..48044)
                     /gene="wcaC"
                     /locus_tag="OQQ48_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HCS1950959.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTVVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEDVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(48041..48529)
                     /gene="wcaB"
                     /locus_tag="OQQ48_000049"
     CDS             complement(48041..48529)
                     /gene="wcaB"
                     /locus_tag="OQQ48_000049"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HCS1950960.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(48532..49371)
                     /gene="wcaA"
                     /locus_tag="OQQ48_000050"
     CDS             complement(48532..49371)
                     /gene="wcaA"
                     /locus_tag="OQQ48_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HCS1950961.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            complement(49549..51711)
                     /gene="wzc"
                     /locus_tag="OQQ48_000051"
     CDS             complement(49549..51711)
                     /gene="wzc"
                     /locus_tag="OQQ48_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HCS1950962.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEDEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKELIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAMGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(51714..52157)
                     /gene="wzb"
                     /locus_tag="OQQ48_000052"
     CDS             complement(51714..52157)
                     /gene="wzb"
                     /locus_tag="OQQ48_000052"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HCS1950963.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISIAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(52163..53302)
                     /gene="wza"
                     /locus_tag="OQQ48_000053"
     CDS             complement(52163..53302)
                     /gene="wza"
                     /locus_tag="OQQ48_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HCS1950964.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKTYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQVLMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            53611..53760
                     /locus_tag="OQQ48_000054"
     CDS             53611..53760
                     /locus_tag="OQQ48_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1950965.1"
                     /translation="MVIEYWLSNNAGGSVTANGIAGKVTIRQLAIFKNYIKCHSIWFL
                     GVSLG"
     gene            53961..55544
                     /locus_tag="OQQ48_000055"
     CDS             53961..55544
                     /locus_tag="OQQ48_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCS1950966.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEDEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLHKDGELEYEV"
     gene            complement(55818..57671)
                     /gene="asmA"
                     /locus_tag="OQQ48_000056"
     CDS             complement(55818..57671)
                     /gene="asmA"
                     /locus_tag="OQQ48_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HCS1950967.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRNYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEVVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIHGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALSGAGTLNLAEQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(57693..58274)
                     /gene="dcd"
                     /locus_tag="OQQ48_000057"
     CDS             complement(57693..58274)
                     /gene="dcd"
                     /locus_tag="OQQ48_000057"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HCS1950968.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(58366..59007)
                     /gene="udk"
                     /locus_tag="OQQ48_000058"
     CDS             complement(58366..59007)
                     /gene="udk"
                     /locus_tag="OQQ48_000058"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCS1950969.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHNLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            59325..62622
                     /locus_tag="OQQ48_000059"
                     /pseudo
     CDS             59325..62622
                     /locus_tag="OQQ48_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(62661..63518)
                     /gene="alkA"
                     /locus_tag="OQQ48_000060"
     CDS             complement(62661..63518)
                     /gene="alkA"
                     /locus_tag="OQQ48_000060"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HCS1950970.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADDYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLFDLQCNPQIVNGALGKLGAAR
                     PGLRLPGCIDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGSLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDGTDEL"
     gene            63652..65004
                     /gene="yegD"
                     /locus_tag="OQQ48_000061"
     CDS             63652..65004
                     /gene="yegD"
                     /locus_tag="OQQ48_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCS1950971.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPHLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFRDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEESKIALSNAAETRASLPFISDELATLISQQGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGISIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(65016..66938)
                     /gene="yegI"
                     /locus_tag="OQQ48_000062"
     CDS             complement(65016..66938)
                     /gene="yegI"
                     /locus_tag="OQQ48_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HCS1950972.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDIEEFVDSVAKIYHTP
                     PPASKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQSYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINVNGTLHLCEVGVSHFTPPELQTLSSFVGFERTENHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPSDVEAMFQQ
                     AFTESGVATGRPTAKAWVAALDSLRQQLKKCPVSAMHVYPAHLTDCPWCTLDNQGVIY
                     FVDLGEEVITTSGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILIEIALSALSLLLCGLQAEPRYIILVPVLSAIWIIGSLTSKAYKAEIQQRREAFNRA
                     KMDYEHLVSQIQQLGGLEGFIAKRAMLEKMKDEILGLPEEEKRALAALYDTARERQKQ
                     KFLEGFFASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWKASC
                     DRRFVFRPNEAVTPADRQAVMAAKRHRLESALTVGATELQRFRLYAPARTMPLMEPLR
                     QAAEKLAQAQADLSRC"
     gene            complement(66935..67696)
                     /gene="pphC"
                     /locus_tag="OQQ48_000063"
     CDS             complement(66935..67696)
                     /gene="pphC"
                     /locus_tag="OQQ48_000063"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase PphC"
                     /protein_id="HCS1950973.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVVWLNDQQPLLVMFL
                     ADGAGSVSQGGEGATLAVNEAMAFMVQKVQDGELGLNDVLATNMVLTIRQRLFAEAEA
                     KALAVRDFACTFLGLISSPDGTLIMQIGDGGVVVDLGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTGRAHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASVTQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(67693..68352)
                     /locus_tag="OQQ48_000064"
     CDS             complement(67693..68352)
                     /locus_tag="OQQ48_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCS1950974.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(68573..68632)
                     /gene="ibsA"
                     /locus_tag="OQQ48_000065"
     CDS             complement(68573..68632)
                     /gene="ibsA"
                     /locus_tag="OQQ48_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HCS1950975.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(68905..68961)
                     /locus_tag="OQQ48_000066"
     CDS             complement(68905..68961)
                     /locus_tag="OQQ48_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCS1950976.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            complement(69233..69289)
                     /locus_tag="OQQ48_000067"
     CDS             complement(69233..69289)
                     /locus_tag="OQQ48_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCS1950977.1"
                     /translation="MMKLLIIVVLLVISWPAY"
     gene            69568..70815
                     /gene="mdtA"
                     /locus_tag="OQQ48_000068"
     CDS             69568..70815
                     /gene="mdtA"
                     /locus_tag="OQQ48_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HCS1950978.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSRSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTIIAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQTQGQLAKDKATLANARRDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGMIKVKARFNNQNDVLFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            70815..73937
                     /gene="mdtB"
                     /locus_tag="OQQ48_000069"
     CDS             70815..73937
                     /gene="mdtB"
                     /locus_tag="OQQ48_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HCS1950979.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLIFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYSAFGQRLISTIYTQANQYRVVLEHNTEITPGLAALDTIRLTSSDGGVVPLSSIAKV
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            73938..77015
                     /gene="mdtC"
                     /locus_tag="OQQ48_000070"
     CDS             73938..77015
                     /gene="mdtC"
                     /locus_tag="OQQ48_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HCS1950980.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGKLYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVEPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            77016..78432
                     /locus_tag="OQQ48_000071"
                     /pseudo
     CDS             77016..78432
                     /locus_tag="OQQ48_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416581.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            78429..79832
                     /gene="baeS"
                     /locus_tag="OQQ48_000072"
     CDS             78429..79832
                     /gene="baeS"
                     /locus_tag="OQQ48_000072"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HCS1950981.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDEQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            79829..80551
                     /gene="baeR"
                     /locus_tag="OQQ48_000073"
     CDS             79829..80551
                     /gene="baeR"
                     /locus_tag="OQQ48_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HCS1950982.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            80742..81074
                     /locus_tag="OQQ48_000074"
     CDS             80742..81074
                     /locus_tag="OQQ48_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HCS1950983.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            81197..82597
                     /locus_tag="OQQ48_000075"
     CDS             81197..82597
                     /locus_tag="OQQ48_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000911981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HCS1950984.1"
                     /translation="MLKGLRTLFSRRVLFNVEMNNSVDCELLLIQQQLQALIAEPGNY
                     LAHDCDLNDTLVLCQLTHHHNGYIREKAVQYLGHKEDVAAIEELLLAANDWVKEVRLA
                     ATIALRHLMKDKNAAAFAVNLPMIRNLLQCQRYDHRAFVDEILRYLLTENNRPQLTYW
                     LTCPDKKLSRAVLQTLIEYGYFNDESSLLSILDKPDEGLRMLATTHWLRQQLPLSEAV
                     LTRLLKDRWPRIRQATLFSLTDRAIEMPPALHSTLLLDNNMLIRLRAKNMLHEVMDVP
                     QFWRHVVTSAEYTPSQRRAALYGLDSIHDPNILKLAEWGLSQNVFPLRLAAMHILAKA
                     NPRCGVKETILTTLANPDAAGLRFMVNICVWCRVPLTFEEIRQLQENAPSVKHACAYC
                     RLYHNLNKWDGLILLLQSQHKLTEEFAGKQLAIWQRNFNLSGIQPNALQRQQLQALFT
                     RNPELHNRLWGYIPFK"
     gene            82918..84279
                     /gene="yegQ"
                     /locus_tag="OQQ48_000076"
     CDS             82918..84279
                     /gene="yegQ"
                     /locus_tag="OQQ48_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HCS1950985.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREYFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGVAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(84609..84926)
                     /locus_tag="OQQ48_000077"
     CDS             complement(84609..84926)
                     /locus_tag="OQQ48_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1950986.1"
                     /translation="MKKIAAISLISIFIMSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARSRISGAENIVKNDATHFCVTQGKKMQIVELKTEGVGLHGVARLTFK
                     CGE"
     gene            85340..86238
                     /gene="yegS"
                     /locus_tag="OQQ48_000078"
                     /pseudo
     CDS             85340..86238
                     /gene="yegS"
                     /locus_tag="OQQ48_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
     gene            complement(86320..87093)
                     /locus_tag="OQQ48_000079"
     CDS             complement(86320..87093)
                     /locus_tag="OQQ48_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCS1950987.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSVSGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(87199..88239)
                     /gene="gatD"
                     /locus_tag="OQQ48_000080"
     CDS             complement(87199..88239)
                     /gene="gatD"
                     /locus_tag="OQQ48_000080"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCS1950988.1"
                     /translation="MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGSMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            complement(88287..89642)
                     /locus_tag="OQQ48_000081"
     CDS             complement(88287..89642)
                     /locus_tag="OQQ48_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCS1950989.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSVSLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFITWRRARGFIKQEKAVLA
                     E"
     gene            complement(89646..89930)
                     /gene="gatB"
                     /locus_tag="OQQ48_000082"
     CDS             complement(89646..89930)
                     /gene="gatB"
                     /locus_tag="OQQ48_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HCS1950990.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQNHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            complement(89961..90413)
                     /gene="gatA"
                     /locus_tag="OQQ48_000083"
     CDS             complement(89961..90413)
                     /gene="gatA"
                     /locus_tag="OQQ48_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HCS1950991.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVEVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            complement(90483..91685)
                     /gene="gatZ"
                     /locus_tag="OQQ48_000084"
     CDS             complement(90483..91685)
                     /gene="gatZ"
                     /locus_tag="OQQ48_000084"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HCS1950992.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKEYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIVRGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQPLAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLEGVDIPLGMISQYLPKQFERIQS
                     GELSAIPH"
     gene            complement(91714..92568)
                     /gene="gatY"
                     /locus_tag="OQQ48_000085"
     CDS             complement(91714..92568)
                     /gene="gatY"
                     /locus_tag="OQQ48_000085"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HCS1950993.1"
                     /translation="MHVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADAFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATEPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            complement(92876..93928)
                     /gene="fbaB"
                     /locus_tag="OQQ48_000086"
     CDS             complement(92876..93928)
                     /gene="fbaB"
                     /locus_tag="OQQ48_000086"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HCS1950994.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVVSTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            94185..95462
                     /locus_tag="OQQ48_000087"
     CDS             94185..95462
                     /locus_tag="OQQ48_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1950995.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMSTIGWIASGLACGFLPQILGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            95459..96463
                     /locus_tag="OQQ48_000088"
     CDS             95459..96463
                     /locus_tag="OQQ48_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HCS1950996.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRTTTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            96460..97425
                     /locus_tag="OQQ48_000089"
     CDS             96460..97425
                     /locus_tag="OQQ48_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCS1950997.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEELQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(97399..98145)
                     /locus_tag="OQQ48_000090"
     CDS             complement(97399..98145)
                     /locus_tag="OQQ48_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCS1950998.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFCSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(98197..99015)
                     /locus_tag="OQQ48_000091"
     CDS             complement(98197..99015)
                     /locus_tag="OQQ48_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HCS1950999.1"
                     /translation="MQLRITSRKKLTSLLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQVRLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVEELQAFVDGIKETP"
     gene            complement(99080..99880)
                     /gene="thiD"
                     /locus_tag="OQQ48_000092"
     CDS             complement(99080..99880)
                     /gene="thiD"
                     /locus_tag="OQQ48_000092"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCS1951000.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            complement(99877..100665)
                     /gene="thiM"
                     /locus_tag="OQQ48_000093"
     CDS             complement(99877..100665)
                     /gene="thiM"
                     /locus_tag="OQQ48_000093"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HCS1951001.1"
                     /translation="MQVDLLGSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTPWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEMDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     regulatory      complement(100700..100796)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(100888..101160)
                     /gene="rcnR"
                     /locus_tag="OQQ48_000094"
     CDS             complement(100888..101160)
                     /gene="rcnR"
                     /locus_tag="OQQ48_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HCS1951002.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     gene            101281..102105
                     /gene="rcnA"
                     /locus_tag="OQQ48_000095"
     CDS             101281..102105
                     /gene="rcnA"
                     /locus_tag="OQQ48_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HCS1951003.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIISTA
                     FWMFWRTWRGERNWLENMHGHDYEHHHHDHEHHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            102324..102662
                     /gene="rcnB"
                     /locus_tag="OQQ48_000096"
     CDS             102324..102662
                     /gene="rcnB"
                     /locus_tag="OQQ48_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HCS1951004.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(102744..103778)
                     /locus_tag="OQQ48_000097"
     CDS             complement(102744..103778)
                     /locus_tag="OQQ48_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HCS1951005.1"
                     /translation="MEIRIMLFILMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     MATYEHNFNDSSCVSTNTITPMSPSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTNGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNAGVYSFNLTLSYNPITTTCKPDDLLITLEGIPVSQLPATGN
                     KATINSKKGDIILRCKNLLGQQNQTSRKMQVYLSSSDLLTNSNTILKGAEDNGVGFIL
                     ESNGSPVTLLNITDNSKGYTNLKEIAAKSKLTDTTVSIPITASYYVYDTNKVKSGALE
                     ATALINVKYD"
     gene            complement(103794..106274)
                     /locus_tag="OQQ48_000098"
     CDS             complement(103794..106274)
                     /locus_tag="OQQ48_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCS1951006.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYL
                     SQYYSDYKASGNNKSTYVRFNSGLNLLGWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQLLGTLRVGDMYTSSDIFDSVRFRGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSEFVQVGYQYGFNNLLTLYGGSMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDVYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNWRRISYTLAASQAYDENHHEEKRFNIFISIPFDWGDDV
                     TTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQAGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVIYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLMFGYEVNDIHGHNIGVVGQGSQLFIRTNEVPPSVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
     gene            complement(106290..106964)
                     /locus_tag="OQQ48_000099"
     CDS             complement(106290..106964)
                     /locus_tag="OQQ48_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCS1951007.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWHLTIIDDHGN
                     YISDKI"
     gene            complement(107044..107586)
                     /locus_tag="OQQ48_000100"
     CDS             complement(107044..107586)
                     /locus_tag="OQQ48_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1951008.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTSVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGDVLSSLVMRVAQD"
BASE COUNT        27254 a        27387 c        25448 g        27537 t
ORIGIN      
        1 cagtgacaaa gggatgccag cgtttttcaa acagaatatg agcgatcccg gttcggcgcg
       61 gcggtaaggt cgcgactttg agttttggct tcttcaactc tttcatcacc acccagagcg
      121 ttatcaccag caaaacggca accaacggcc acacagacaa attaaaagtc tcagcaatag
      181 agttatgttc agtactgtag tgctgcaagg tttgatttaa accactggca tgtggagaac
      241 gcatcaccgc actcatcacc atataagtga aaagcgcgat ccaactgccg atcaatccct
      301 cacccgcgcg ataccaggtc ccggtggcac atccacccgc cagaacaatt cccagcccga
      361 agatataccc acctataaca gtaccaggcc acgggaacgc cccggcttcg taagtcagta
      421 ggcccgccta aattaacgca aaaaccccca cactttgtac cgaaattgca atcagcaagg
      481 cgtaaaacat gcgattattt ttcgcgatat acatatcgcg aaaaccacca gtcaggcaaa
      541 aacgcccacg ctgcatgaca aatcccagca gagcaccaca aattagcccg cttaatatca
      601 ttgaaaacat aaattaatta accagatgaa tgttaatgag gaaattattc atgactggtg
      661 gaatgcagac caataaccaa attctctaaa ttagaacaaa tggttcttaa tgaggatttc
      721 gataaaaaag aataaaaaaa ccggagccaa atattcattc gactccggtt gattattaga
      781 aaagatgatt atgctacgtc ttcgtactgc ggaaccgggt tacggaagct cttggtgacg
      841 caagccaggt aaatcagacc gatagcagcc cagatcagac ccagaaccat tgagctttct
      901 tccaggttaa cccacagcgc accaacggtc agcgcaccac acatcggcag gaacagatac
      961 tggaagtgat ctttcagcgt cttattacgc ttctcacgga tccagaactg cgagatgacc
     1021 gacaggttaa cgaaggtgaa cgccaccagc gcaccaaagt taatcagcgc cgttgccatt
     1081 accaggtcga agttgattgc cagcagggca atcgcgccaa ccaggatgat gttcatcgcc
     1141 ggagtacgcc atttcgggtg tacataaccg aagaagcttt tcgggaatac gccgtcacga
     1201 cccattacgt acatcagacg cgctacgcct gcatgcgccg ccataccgga cgccagtacg
     1261 gtaatggtgg agaagatcag cgcgccaacc tggaacgctt tacccgcaac gtacagcatg
     1321 atttcaggct gtgacgcatc cggatcttta aagcgagaga tatccgggaa gtacagctgc
     1381 aggaagtaag ttgcaaagat gaagatcatg ccgccgatca gcgcggtcag gaaaatcgca
     1441 cgcgggatca cgcgctctgc atctttggtt tcttccgaca ggttgctgat gccgtcaaag
     1501 ccggtaaagg agaagcacag gattgtcgcc ccggtaatca tcgggataac atgcgcatcg
     1561 ccagaccaga atggacgagt gctcgccagc gtaccagcgc cttcaccttc aaatacgcca
     1621 taaacaacca tgcccagaat caccgcgatc agcaccacct gcaacacgac gattacagtg
     1681 ttgaagttcg ctacggattt cagactacgc aggttaaagg cggtcatgaa ggccaccaac
     1741 gccaccacaa acatccacga tgggatggaa ggcaccagag cttcaaaata gattttcgcc
     1801 agcagaatgt tgatcatcgg cgcgaacaga tagtcgagca gagaagacca acccaccata
     1861 aagccgacag tcgggctaat ggatttctgg gcgtaagtgt atgcagagcc agcagaagga
     1921 tagcggcgaa ccagcttccc gtagctcaga gccgtaaaca ggatcgcaat caatgcgaac
     1981 gcataggctg tcggcacatg accatccgtg aggcctgaaa cgataccaaa agtatcaaac
     2041 agcgtcatcg gctgcatata ggcaagaccc atcattacaa ccggaactaa cgtaagcgtt
     2101 ttacgcaatt ccacgcgaga ggtgtttgga gtaacgttat gcgacatggt cattctcctt
     2161 tacggtgata accgtcgcgt aagcaaaaaa ttgccccatt tttttggatt cctcagcgac
     2221 aacaactgtc gatttttagt aaatatctat ccggtacgaa gcccggcctc ttggtatgaa
     2281 tgattggttt gaagcaaaaa ataaccgacg ctgatgaaac gtcggttttt agtcattttt
     2341 tgacagcggc gcattgtgcc taaatggggg ggaaatgaca agagaatgag aggcttgtca
     2401 taataatttt tctttaaatg gctgattttc cgtcatcaga ttttgcataa acaccggcga
     2461 tcgcactatt tgctaaaatt tcatcccgcc accagccact ggcaagtcct gcggtctgtt
     2521 cattccagcc aatccagacg ggttcaaaga gagtttgggc gaccactttc ttctcgatta
     2581 acgcgccact atcgagaaaa tgttgcgcca gataacgggg taaatagccg cagccgaggc
     2641 cgctaatttg cagctccagc ttggttttaa aatcgaaaac ggtaatggct tcttgttcgt
     2701 cgagaagttg tgaggccgta gaagcgcctg cctgcgcagt atcccccacc acaatcgccc
     2761 gatatcgctt aataatacga cgatttaacg gctcttcttc cagggctaac ggatgatgag
     2821 gcgcgaccgc aaagacttgt tccagatcgc ccaaccgtga aaaaccaaac tcactggaag
     2881 atggtggctc atgcatcgcg ccaacaataa tatccgctcg cccctgagtc agggcatccc
     2941 atgaaccacc cagtacgcca ttgataaact tcaggcgcgt cacgctatga tgttgataaa
     3001 aagcttcaat cagtggcgct agcagagaaa aaggaaacgt gtcgtccacg ccaatcacca
     3061 gctcgttttc ccagccttca tgcagtttga tcgcctgctt ttccagttcc cgcacggtat
     3121 gcagaacttc ccgccctttc tctaataaca tttttccggt gcgggtgaat ttagcgcggt
     3181 gaccgctacg atcaagcaat tgaatattaa ggtcgctttc cagcttgtga acggtgtaac
     3241 tcagagcgga cggcgtcttg tagagtttgg ccgacgccgc cgcaaaactg ccttcttttt
     3301 ctaaggcatc gaggatcatc aacacatcca gcagtggttt catacacgcc cccttgcagt
     3361 gtatgcggcg tcctgctggc gtgatcgttt actccagcgg cattaccagc ggatcgggat
     3421 actggtattc aaatcccagt tcattacaaa tccgactgcc atcaataatc ttacctttgc
     3481 cgctgtccag actatttctg aactgcggag gttccagccc cagtaaacgg gccatctgcg
     3541 gatagaaaac attacgcgca gggtgagctg gcgcacatat attatagatg tgtccgcctt
     3601 taggtgcctg taacaacaga gtgatagcgc caataacatc ttctaaatgg actaaattaa
     3661 caccatgttc accatcaggc gcggtttttc cggcaaagaa gcgtccggga tgacgccccg
     3721 gtccgaccag gcccgcaaga cgcagaatat cgaccgaagt accgggtaaa ttgtgcagcc
     3781 agtcttcgag ttcttctaat actcgtccac tgttggttac cggattacgc ggggtagttt
     3841 ctttcaccat gccttgcgca tcgccataga cagatgttga gctggtaaaa ataattcggg
     3901 gaatacgatg ggccagcgcg ctatccacta actcttgtac cgcttgtaaa tagaactcat
     3961 cgccggagcc gctacgacgt gccggaagcg taatgaccag tgcatcggca tccatcaggg
     4021 catccagatc gtcagaatcg caaactaact caggctccat gcgaagcaga tagctatcaa
     4081 tgccactcat tcgggccgct tcgacaccat cttgtgtggt tttactcccg gtgacttgcc
     4141 agcctcgcgc tgaaagtgac atcgccagcg gcatgcctaa ccaccctaac ccgacaattg
     4201 cgaccttttt catccgttat ctcctgatac accttagatc tataaggcta cgctagcgta
     4261 tcaaaactga caattcattc tatgaatgaa tctgttcaat aatgataacg acatgctgca
     4321 atgagcagtg aatcgtcggt aaccgcgtat accagacggt gctcctctgt aatgcgtcgg
     4381 gactagaaac ctgacaaatt atgtttcagg ggttctggct tccccttacc ttcaaatggc
     4441 gttctgcggg tatctttgat aagttcattg atctttttaa caattcgctt atctgtttcc
     4501 tgccagtaca gataatcatc ccatgattcc tcagaccaga ttagtttcac tcaataatgt
     4561 ccttttctgt tcctttgcct gatttcaggc tatcgattga gtccatcaat ctccgggcgt
     4621 tagcggggga acgcagtaga taagccgtct cttccagcga gttgtattct tcgagtgaca
     4681 tcagaacaca agcctcccca ttctgacgag taataaggat cggggcatga tcttcaacgg
     4741 ctttcatcat tgttgccgac aaattctgac gcgcttcgct gtagctaatt gtacgcatgt
     4801 caatctcctc ttttgtacag ttcattgtac aatgatgggc gttaattaac tatttattaa
     4861 ttagtttgta gatcaaggta ttgtcagtga gacgaaaatc caggcttcgc tatttttggt
     4921 gccgtcagct tagaggacag tcctcttagc cccctccttt ccccgctcat tcattaaaca
     4981 aatccatcgc catagaatat ataaaaaagc ccttgctttc taacgtgaaa gtggtttagg
     5041 ttaaaagaca tcagttgaat aaacattcac agagactttt atgacacgcg ttcaatttaa
     5101 acaccaccat catcaccatc atcctgacta gtctttcagg cgatgtgtgc tggaagacat
     5161 tcagatcttc cagtggtgca tgaacgcatg agaaagcccc cggaagatca ccttccgggg
     5221 gcttttttat tgcgcggttg ataacggttc agacaggttt aaagaggaat aagaaaatga
     5281 cagacaacac tcgtttacgc atagctatgc agaaatccgg ccgtttaagt gatgactcac
     5341 gcgaattgct ggcgcgctgt ggcattaaaa tcaatcttca cacccagcgc ctgatcgcga
     5401 tggcagaaaa catgccgatt gatattctgc gcgtgcgtga cgacgacatt cccggtctgg
     5461 taatggacgg cgtggttgac ctcgggatta tcggcgaaaa cgtgctggaa gaagagctgc
     5521 ttaaccgccg cgcccagggt gaagatccac gctactttac cctgcgtcgt ctggatttcg
     5581 gcggctgccg actttcactg gcaacgccgg ttgatgaagc ctgggatggc ccgctctcct
     5641 taaacggtaa acgtatcgcc acctcttatc ctcacctgct caagcgttat ctcgaccaga
     5701 aaggtatctc ttttaaatcc tgcttactga acggttctgt tgaagtcgcc ccgcgtgccg
     5761 gactggcgga tgcgatttgc gatctggttt ccaccggtgc cacgctggaa gctaacggcc
     5821 tgcgcgaagt cgaagttatc tatcgctcga aagcctgcct gatacaacgc gatggcgaaa
     5881 tggaagaatc caaacagcaa ctgatcgaca aactgctgac ccgtattcag ggtgttatcc
     5941 aggcgcgtga atcaaaatac atcatgatgc acgcaccgac cgaacgtctg gatgaagtca
     6001 tcgccctgct gccaggtgcc gaacgcccaa ctattctacc gctggcgggt gatcaacagc
     6061 gcgtagcgat gcacatggtc agtagcgaaa ccctgttctg ggaaacgatg gaaaaactga
     6121 aagcgctggg tgccagttca atcctggtcc tgccgattga gaagatgatg gagtgatcgc
     6181 catgagcttt aacacaatca ttgactggaa tagctgtact gcggagcaac aacgccagct
     6241 gttaatgcgc ccggcgatct ccgcttctga aagcattacc cgcactgtta acgatattct
     6301 cgataacgtg aaagcacgcg gcgatgaggc cctgcgggaa tacagcgcga agtttgataa
     6361 aaccacggtt accgcactga aggtgtctgc tgaggaaatt gccgccgcta gcgaacgcct
     6421 gagcgacgag ctaaaacagg cgatggcggt ggcagtgaag aatattgaaa ccttccacac
     6481 tgcgcaaaaa ctgccgccgg tagatgtaga aacgcagcca ggcgtgcgtt gccagcaggt
     6541 cacgcgcccg gtcgcttcag ttgggttgta tattcctggc ggctccgccc cgctcttctc
     6601 aacggtatta atgctggcga ctccggcgcg tattgcgggc tgtaaaaaag tggtgctgtg
     6661 ctcaccgccg ccgattgccg atgagatcct ttatgcggcg cagctgtgcg gtgtgcagga
     6721 cgtgtttaac gtcggcggcg cacaggccat tgccgcactg gcgtttggta cggaatctgt
     6781 gccgaaagtg gacaaaatct tcgggccggg caacgccttt gtcaccgaag cgaaacgtca
     6841 ggtgagccag cgtctggacg gtgcggcgat cgatatgccc gcaggcccgt cggaagtgtt
     6901 ggtcattgcc gacagtggcg caacgccgga tttcgtggct tctgatttgc tttctcaggc
     6961 tgaacacggc ccggactcac aggtgatttt actgacgccc gctgctgata tggcgcgtcg
     7021 cgttgccgag gctgtcgaac gccagctggc agaactgccg cgtgctgaaa ccgcccgtca
     7081 ggcactgaac gccagccgcc tgatcgtgac taaagattta gcgcagtgcg tagagatctc
     7141 caaccagtac ggcccggagc acctgatcat ccagacccgc aacgcccgcg atctggtcga
     7201 tggcattacc agtgccggtt cggtatttct tggtgactgg tcactggaat cggcaggtga
     7261 ttacgcctcc ggcaccaacc acgttctgcc gacttacggt tacaccgcca cctgttccag
     7321 cctcgggctg gcagatttcc agaagcgcat gaccgtgcag gaactgtcga aagaaggttt
     7381 ctccgcgctg gcttcgacca ttgaaacatt ggccgccgcc gagcgcctga ccgcccataa
     7441 aaatgccgtt actttacgtg ttaacgccct taaggagcaa gcatgagcat cgtgactatt
     7501 accgatttag cgcgtgaaaa cgtgcgcaac ctgacgccgt atcagtcggc gcgtcgtctg
     7561 ggcggtaacg gcgatgtctg gctgaacgcc aacgaatatc ccacagccgt ggaatttcag
     7621 cttactcagc aaacgctcaa ccgctacccg gaatgtcagc cgaaagcggt gattgaaaat
     7681 tacgcgcaat atgcaggcgt aaaaccggaa caggtgctgg tcagccgtgg cgcggacgaa
     7741 ggtattgaac tactgattcg cgctttttgc gaaccgggta aagacgccat cctctactgc
     7801 ccgccaacgt acggcatgta cggcgtcagc gccgaaacca ttggcgtcga gtgccgcaca
     7861 gtgccgacgc tggacaactg gcaactggat ttacagggca tttccgacaa gctggaaggc
     7921 gtaaaagtgg tctatgtttg cagccccaac aacccgaccg ggcaactgat caatccgcag
     7981 gattttcgca ctctgctgga gttaacccgc ggtaaagcga ttgtggttgc cgatgaagcc
     8041 tatatcgagt tttgcccgca ggcatcgctg gctggctggc tggcggaata tccgcacctg
     8101 gctattttgc gcacactgtc gaaagctttt gctctggcgg gccttcgttg cggatttacg
     8161 ctggcaaacg aagaagtcat caacctgctg atgaaagtga ttgcccccta cccgctctcg
     8221 acgccggttg ccgacattgc ggcccaggcg ttaagcccgc aggggatcgt cgccatgcgt
     8281 gagcgggtgg cgcaaattat tgcagaacgc gaatgcctga ttgccgcact gaaagagatc
     8341 ccctgcgtgg agcaggtttt cgactctgaa accaactaca ttctggcgcg ctttaaagcc
     8401 tccagtgcag tgtttaaatc tttgtgggat cagggcatta tcttacgtga tcagaataaa
     8461 cacccctctt taagcggctg cctgcgaatt accgtcggaa cccgtgaaga aagccagcgc
     8521 gtcattgacg ccttacgtgc ggagcaagtt tgatgagtca gaagtatctt tttatcgatc
     8581 gcgatggaac cctgattagc gaaccgccga gtgattttca ggtggaccgt tttgataaac
     8641 tcgcctttga accgggcgtg atcccggagc tgctgaagct gcaaaaagcg ggctacaagc
     8701 tggtgatgat cactaatcag gatggtctgg gaacacaaag tttcctgcag gcggattttg
     8761 atggcccgca caacctgatg atgcagatct tcacctcgca aggcgtgcag tttgatgaag
     8821 tgctgatttg tccgcacctg cccgccgatg agtgcgactg ccgtaagccg aaagtaaaac
     8881 tggtggaacg ttatctggct gagcaagcga tggatcgcgc taacagttat gtgattggcg
     8941 atcgcgcgac cgacattcaa ctggcggaaa acatgggtat taatggttta cgctacgacc
     9001 gcgaaaccct gaactggccg atgattggcg agcaactcac taaacgagac cgttacgccc
     9061 atgtagtgcg caacaccaaa gagacgcaaa ttgacgtcca ggtgtggctg gatcgcgaag
     9121 gtggcagcaa gattaacacc ggcgttggct tctttgatca catgctggat cagatcgcca
     9181 cccacggcgg tttccgtatg gaaatcaacg tcaaaggcga cctctatatc gacgatcacc
     9241 acaccgtcga agataccggc ctggcgctgg gtgaagcgtt aaaaattgcc cttggcgaca
     9301 aacgcggtat ttgccgcttt ggttttgtgc taccgatgga cgaatgcctt gcccgctgtg
     9361 cactggatat ctctggtcgc ccgcacctgg aatataaagc cgaatttacc taccagcgcg
     9421 tcggcgatct cagcaccgag atgatcgagc acttcttccg ttcgctgtct tacaccatgg
     9481 gcgtgacgct acacctgaaa accaaaggta aaaacgatca ccaccgtgta gagagcctgt
     9541 tcaaagcctt tggtcgcacc ctgcgccagg ccatccgcgt ggaaggcgac accctgccct
     9601 cgtcgaaagg agtgctgtga tgaacgtggt gatcctggat accggctgcg ccaacctgaa
     9661 ctcggtgaag tctgccattg cgcgccacgg ttatgaaccc aaagtcagcc gtgacccgga
     9721 cgtcgtgttg ctggccgata aactattttt acccggcgta ggcaccgcac aagcagcgat
     9781 ggatcaggtg cgtgagcgcg aactgtttga tctcatcaaa gcctgtaccc aaccggtgct
     9841 gggcatctgc ttagggatgc aactgcttgg gcggcgcagc gaagagagca acggcgtcga
     9901 cttgctgggc atcatcgacg aagacgtgcc gaaaatgacc gactttggtc tgcccctgcc
     9961 gcatatgggc tggaaccgcg tttacccgca ggcaggcaac cgcctgtttc aggggattga
    10021 agatggcgcg tacttttact ttgttcacag ctacgcaatg ccagtcaatc cgtggaccat
    10081 cgcccagtgt aattacggcg aaccgttcac cgcggcggta caaaaagata acttctacgg
    10141 cgtgcagttt cacccggagc gttctggtgc cgctggcgct aagttgctga aaaacttcct
    10201 ggagatgtga tgattattcc ggcattagat ttaatcgacg gcaccgtggt gcgtctccat
    10261 cagggcgatt acggcaaaca gcgcgattac ggtaacgacc cgctgccgcg cttacaggat
    10321 tacgcagcgc agggtgccga agtgctgcat ctggtggatc tgaccggagc aaaagatccg
    10381 gcaaaacgtc aaatcccgct gattaaaacc ctggtcgcgg gcgttaacgt tcctgtgcag
    10441 gttggcggcg gcgtgcgtag cgaagaagac gtggcggcgt tactggaagc gggcgttgcg
    10501 cgcgtggtgg ttggctccac cgcggtgaaa tcaccagaaa tggtgaaagg ctggtttgaa
    10561 cgtttcggtg ccgatgcctt agtactggcg ctggatgtcc gtattgacga gcaaggtaac
    10621 aagcaggtgg cggtgagcgg ctggcaagag aactcgggcg tttcactgga acaactggtg
    10681 gaaacctatc tgcccgtcgg cctgaaacat gtgctttgta ccgatatctc gcgcgacggc
    10741 acgctggcag gttctaacgt ctctttatat gaagaagtgt gcgccagata tccgcaggtg
    10801 gcatttcagt cctccggcgg cattggcgac attaacgatg ttgctgctct acgtggtact
    10861 ggcgtgcgcg gcgtaatagt tggtcgtgca ttactggaag gtaaattcac cgtgaaggag
    10921 gccatcgcat gctggcaaaa cgcataattc cgtgtctcga cgttcgtgat ggtcaggtgg
    10981 tgaaaggcgt acagtttcgc aaccatgaaa tcattggcga tatcgtgcca ctggcaaaac
    11041 gctacgccga agaaggtgca gatgaactgg tgttctacga tatcaccgct tccagcgatg
    11101 gccgtgtggt agataaaagc tgggtatctc gcgtggcgga agtgatcgac attccgtttt
    11161 gtgtggcggg tgggattaag tctctggaag atgccgcgaa aattctttcc tttggcgcgg
    11221 ataaaatttc cataaactcg cctgcgctgg cggacccgac gttaattact cgcctggccg
    11281 atcgctttgg cgtgcagtgt attgtagtcg gtattgatac ctggtacgac ggcgaaaccg
    11341 gtaaatatca tgtgaatcaa tataccggcg atgaaagccg cacccgcgtc actcagtggg
    11401 aaacactcga ctgggtacag gaagtgcaaa aacgcggtgc cggagaaatc gtcctcaaca
    11461 tgatgaatca ggacggcgtg cgtaacggtt acgacctcga acaactgaaa aaagtgcgtg
    11521 aagtttgcca cgtcccgcta attgcctccg gtggcgcggg caccatggaa cacttcctcg
    11581 aagccttccg cgatgccgac gttgacggcg cgctggctgc ttccgtattc cacaaacaaa
    11641 taatcaatat tggtgaatta aaagcgtacc tggcaacaca gggcgtggag atcaggatat
    11701 gttaacagaa caacaacgtc gcgaactgga ctgggaaaaa accgacggac ttatgccggt
    11761 gattgtgcag cacgcggtat ccggtgaagt gttaatgctg ggctatatga acccggaagc
    11821 cttagacaaa accatcgaaa gcggcaaagt caccttcttc tcgcgcacta aacagcgact
    11881 gtggaccaaa ggcgaaacgt cgggcaattt cctcaacgta gtaaatattg cgccggactg
    11941 cgacaacgac acgctactgg tactggcgaa tcctatcgga ccaacctgcc acaaaggcac
    12001 cagcagctgc ttcggcgaca ccgctcacca atggctgttc ctgtatcaac tggaacaact
    12061 gctcgccgaa cgtaaatctg ccgacccgga aacctcttac accgccaaac tgtatgccag
    12121 cggcaccaaa cgcattgcgc agaaagtggg cgaagaaggc gtggaaaccg cactggccgc
    12181 gacggtacat gaccgctttg agctcaccaa cgaggcgtct gatttgatgt atcacctgct
    12241 ggtattgttg caggatcagg atctggattt aacaacggtg attgagaacc tgcgtaaacg
    12301 tcatcagtga gttgcggggt aagcggatgc gatgttgtcg tcgcatccgg caaaaaaaac
    12361 gggcaaggtc tcaccaccct gcccattttc tttaaaaccg aaaagattac ttcgcgttgt
    12421 aattacgcag agcgttacgc cccagcacaa tccccgcgcc aaccatgccg cccagcagca
    12481 ctgccaaaat caaggtaatt gcctttttcg ggctatcgcg acgaataggt aacgtcggtt
    12541 tcattacata gcggtaagca tgaatatcaa gatcatcaac ctttaagctt tcgatatcca
    12601 gcaggttttg acgtgtctga tagtagtttg atgagaacac caacggacgg gtcgcctcat
    12661 gcttaatcat cgactccagc gcttcgctcc ccaaaaggaa caacgtatct tgtgtcacat
    12721 cttcagtctg ctgaacctgt ggctttgtca cctgcgcctg attcgcatac tgcaacgctt
    12781 cctgaatctg acggatacgc agatctttct gctcctgcgc gactacttcc tgagttttca
    12841 gagaatcctg caagtttttc atacgcagcg cgatgttgtc tttcaggtct ttttccagtt
    12901 cttcgtttac ctggtcgtca acttgctgga tatactgcgc cagctgtttc tgcgcacctt
    12961 cagctgtttc accaacataa gaaaccgcta aaggtaagga ctgattttta acggtaggct
    13021 caatggtcag tttttcaggt tcttcctgat tatccagcgt ttccgctaat gctgagaaag
    13081 cagtgctgta acgtccgata agtgaagtct gaatgtcgga gactttcggt acagccggac
    13141 cgtaaatcac attcatcgcg ttggtataac cagcgatttg ccccacatca ggttgagtaa
    13201 cgatagctgt ggaagtccac ttttctttgg caacggccag ataccctata gccagggcaa
    13261 tagccacaat gacagagatg ataattgtca tcttgccgcg ccacaactgc accaggaaat
    13321 caatcaaatc aatctgttcc gggtcatggt tttgcccaga aacattatta ttttctactc
    13381 tcatcattac cctaacggac gaaataattt taagtcgcca tagtttagcg ataatcgcca
    13441 tgaatgctat aggataaatg ataaaaataa tgaattatta caaagggaac ataagagaat
    13501 cagggcgaaa atcgcactga taacaagatg ttagtcgctg ccaaaaagat cgcgggtgta
    13561 gactttgtct gccacatcct taagctcttc tgccatacgg ttggagataa tgacgtcggc
    13621 ttgtatagtc agcttagaac agctttgatt gtaaaatgag cacgttacca gaaaaaacag
    13681 ccaggtttag aactgatacc gattatcttt ttttcttgtc taacggtatg atttaacttt
    13741 cagttatgcc agatgaaaat tggctatatt ctagcctgag cgaggattat aaatgaaatt
    13801 tctggttacg ggagctgctg gctttatcgg tttctatgta agtaaacggc ttcttgcagc
    13861 tggtcatcag gttgtaggga ttgacaactt aaatgattat tacgatgtca acctcaaaca
    13921 agcacgactt gatttactca agcacgacaa tttcagtttt tataaaattg acctggccga
    13981 tcgcgagaaa atggccgcac tgtttgcaga cgagcggttc gatcgcgtaa taaacctcgc
    14041 tgcgcaagcc ggtgtacgtt actcccttga aaatcccaat gcatatgcag atgcaaacct
    14101 gattggattc ctgaacatac tggaaggatg ccgccataat aatgttcagc atctacttta
    14161 cgcttcctcc agttctgttt atggcatgaa ccgcaagatg cctttctcta cagatgactc
    14221 tgtagatcat cctgtttcgc tttatgcagc aactaaaaaa gcgaatgaac tcatggccca
    14281 tacgtattct catttgtatg gcttaccgac cacagggctg cgtttcttta cggtttatgg
    14341 tccatgggga cgtccggata tggcattatt taaatttact aaagccatgc tggaaggtaa
    14401 aagcattgat gtttacaact tcggcaaaat gaagcgtgac tttacttata ttgatgatat
    14461 tgccgaagct attattcgct tacaggatgt tattccagaa aaagatccac agtggactgt
    14521 agaaactgga tcgcctgcaa caagttcagc accgtatcgt gtctataaca ttggtaatag
    14581 ttcgcctgtg gagttgatgg actatatcaa tgccttagaa gaggctctgg gtattgaagc
    14641 caacaagaat atgatgcctc tccaacccgg tgatgtactg gaaaccagtg ctgatacaaa
    14701 agcactgtat gacgtaatag gattcaaacc tgaaacgtca gttaaagagg gggtaaagaa
    14761 ctttgtagaa tggtatcgta acttctataa agtttgattt tacaaaacca taagaaaagg
    14821 ccctaatata ttagggcctt ttcttagaat aaaacaaaat aattaatcat tgccaaacaa
    14881 gtcgcgcgta taaactttat ctgctacatc agccagatcg gcagacatac ggttagaaat
    14941 aataacatca gcttcttgtt tgaacgcatc cagatcacgt accacgcgcg aacggaaaaa
    15001 atcgtcctct ttcatcgctg gttcataaac gattactggc acacctttcg ccttgattcg
    15061 cttcataata ccctgaatcg aggaagcacg gaaattgtct gaaccactct tcataatcag
    15121 acgataaacg ccaacaactt tcggtttacg tgcaaggata gaatcggcaa taaagtcttt
    15181 tcgcgtgcgg ttagcatcaa caattgccga aatcagatta ttcggtacag attgatagtt
    15241 tgccagtaac tgcttggtat ctttcggcag acaataacca ccataaccaa atgagggatt
    15301 gttgtagtga ttaccgatac gcggatcaag gcaaaccccc tcaataattt ggcgggagtt
    15361 aagacccaga ctttcagcgt aactatctag ttcattaaaa taagccacac gcatcgccag
    15421 ataagtattg gcaaaaagtt taatcgcctc agcctcggtt gagtcagtaa acaatattgg
    15481 tatgtcttgc ttaatagcgc cttcctgtaa taacgctgca aaacgttcag cgcgatcgga
    15541 ttgctcacca atcacaatgc gtgatggatg caagttatca taaagagctt tcccttcgcg
    15601 taaaaactcc ggcgaaaaga tcacattttc aataccaaac cgttctttaa tggactctgt
    15661 aaaaccaaca gggatagttg attttataat cattaccgcg ttgggattaa tttctgtcac
    15721 atcgcgaatg accgcttcca cgcttgaggt attaaaataa tttgttttcg ggtcataatc
    15781 ggtaggcgtg gcaataataa cataatcggc atttttatac gcgtcatact tatctgtcgt
    15841 agcgcggaaa ttgagatctt tagtcgccag atactcttca atctccttat caacaatcgg
    15901 tgactgcttc ttgttaagca tgtctacttt ggcctgaacg atatccagtg caaccacttc
    15961 gtgattttgc gcaatcagaa taccatttga aagaccaaca taacctgttc ctgaaattgt
    16021 tattttcatt agctctgact tcttccggtt aaacatttag agtggtcatt aatccaccac
    16081 cacaacttca tctactgcgg gatttttaac gctaatgtag atgaactgtc aaggcagcga
    16141 tcctgctgtg cggcgctgta ttatatcgcg tttttaacta taaattataa aaaaaggccc
    16201 taacctgccg ctttgtataa taaaaaagcc cggagggttt ctccgggcct tgcattgatt
    16261 aattgattta aatcagatta atccagccat tcggtatgga acacaccgtc tttatcaatg
    16321 cgtttataag tatgcgcacc gaaatagtca cgctgtgcct ggatcaggtt cgcaggcaga
    16381 acagcggcac ggtagctatc ataataggca acagcagcgg cgaaggtcgg aaccgggata
    16441 ccgttctgta ctgcgtaagc gacgacatcg cgcagcgcct gctggtagtc atcggcgatt
    16501 tgcttgaagt acggggccag cagcaggtta gcgatctgcg gattttcggc atatgcatcg
    16561 gtgattttct gcaggaactg cgcacggatg atgcagccag cacggaaaat cttcgcgatt
    16621 tcgccgtagt tcagatccca gttgtactct tcagacgccg cacgcagttg agagaacccc
    16681 tgagcgtaag aaacgatttt gcccagatac agtgcacggc gaactttttc gatgaactca
    16741 gccttgttgc cagctggctg cgcttgtggg ccagagagaa ctttagacgc agcaacgcgc
    16801 tgatctttca gagaagagat gtatcgtgca aacacagact cggtaatcag cgacagcggt
    16861 tcgccgagat ccagtgcgct ctggctggtc catttgcccg tacctttgtt tgccgcttca
    16921 tccaggatca catcaaccag gtagttaccg tcttcatctt ttttagtgaa gatgtctttg
    16981 gtgatatcga tcagatagct gctcagttca ccgttattcc actcggtaaa ggtctgcgcc
    17041 aactcttcgt tggaaaggtt caggccgcct ttaagtagag aataggcttc agcaatcagt
    17101 tgcatatcac cgtattcaat accgttgtga accatcttca catagtgacc tgcaccatcg
    17161 gcaccaatat aggtaacgca tggctcaccg tcttcagcca ctgcggcgat tttggtcagg
    17221 atcggtgcaa ccagttcata ggcttctttc tgtccgccag gcataatgga aggacctttc
    17281 agcgcacctt cttcaccgcc ggaaacaccg gtaccaatga agttaaagcc ttctgcagaa
    17341 agctcacgat tacgacgaat ggtgtcctgg aagaaggtat taccaccatc aatgatgatg
    17401 tcacctttat cgaggtatgg cttgagggaa tcaatagcag catccgtgcc tgcacctgct
    17461 ttcaccatta acaggatgcg acgaggcgtt tccagagatt caacaaactc tttcaccgta
    17521 tagtaaggaa ccagtttctt gcctggattt tcggcaatca cttcttccgt cttctcacgg
    17581 gaacggttga aaatagagac ggtataacca cggctttcga tgttgagcgc aaggttgcgc
    17641 cccatcactg ccataccgac gacgccgatc tgttgctttg acattgttta ctcctgttag
    17701 ggggttacta cattaaatat ttaattaata atattaataa cgccaaaaca acttaatcat
    17761 gtaatatttt atttacacaa ttcagtgctg aacggataac ttggtgcata tcataatact
    17821 tatattcagc aaggcgtcct ccgaaaatta ctttactctc catttgcgaa agctctctat
    17881 atttatgata tatatccata ttttttttat cattaatggg ataataaggc tcttgaccct
    17941 cactccactc tattgggtac tcttcagtta taaaggtata ttcagtttca taagagtcat
    18001 caaaatgctt atgctcaatt atcctagtaa aaggaatatc tttttcagta taattaatta
    18061 cggcattgcc ttgataatta gcctcctcaa ttttttttgt tataaacttt aaggatctat
    18121 attctaaagt tcccaacttt ttatcaaaat aagcatctat aggcccagta tatataatcc
    18181 gttccgccat agaattatac ttatcctttt ctgataaata gtcaacatta agcagaagat
    18241 caacaccatt aagcatctta tcaattattt gtgtgtatcc tccgatagga accccctgaa
    18301 atctatcggt gaaataatta ttatcgaaag tgaatcgaac tgggattctt tttataataa
    18361 aaggcggtaa ttcagaacat gaacgtcccc attgtttttc cgtatacccc ttaattagtt
    18421 tttcataaac atcttcccct accagggaaa gagcttgttc ttctaaattt gtgggagtta
    18481 tgttagaata tttacttctt tgtaaatcta ttttatcttg agcttcagaa ggagtttttg
    18541 tcccccataa ctgataaaag gtattcatat tgaaaggtaa gttatataat ttacctttat
    18601 gatatgctaa tggagaatta gtgaatctat taaaagaagc gaatttattt atataattcc
    18661 agatatattg atcgtttgta tgaaatatat gagcaccata cttatgtatg tgaatatcgt
    18721 caactttttc agtatagata ttccctccaa tatgatccct tttctcaatg accaacacac
    18781 gtttatttct tttatttaat tcataagcgc aaaccgcacc aaataaacct gaacctataa
    18841 ttatgaattc atattgcatt gcggttccta tgtcatctaa cttagttaaa attaaagttc
    18901 tttaagtaag caacctttca tatctttatc tgataatata ggattactta aaggccattg
    18961 aatattgagt tctggatcat tccataataa acaacactca tcctcaggat cataatattc
    19021 tgtgcattta tattcaaaat cggccatttc agataataca acaaaaccat gtgcgaaacc
    19081 tggtggtacc caaaattgtt ttttattcat ttcagaaagt aacacaccaa tccattggcc
    19141 ataattaggt gattcttttc taatatcgac ggcaacatca aaaacttcgc cgcgcacaac
    19201 tcttacaagt ttaccttgtg gcttcgtttt ttggaaatgt aatcctctta aaacattttt
    19261 gcttgatcgt gaatagttat cttgtacgaa atcaaattct atatttaata aattttgata
    19321 acgttgtttt tgatatactt ctaaaaaaaa acccctgttg tcaccatata cgaccggttc
    19381 aataatttta actccatcca gaacagtgtc ttgaactttc atatttatta taacctttag
    19441 tgatttctat ttaaaacgtc attaattgta ttagtatact tctctatgat cttattttca
    19501 tcacaatgtg attcagcaaa ttctctcgac ttaatagaca ttgtgttttt catttcgtct
    19561 gtcagtagaa tatacttttc aataatgtca actaagctat caagatcatt aggttgacac
    19621 aaaaaaccat taacttcatt ttgcaccata tccctacagc caacattatc tgttgtcaaa
    19681 atagctaacc cactagaagc accttcaatc aacgctttag gtacaccttc tctataaaat
    19741 gatggtaata ctattgcatc ataatgcttc attatactag caacgtcatc ggaagtccca
    19801 tagtaattaa ctatacccct cgtacaaaaa ccttcaatca attccttcgt gattatcttt
    19861 tcattttttt caaatgcacc taacagtgaa aatgaacaat taggatattt ggattttatt
    19921 atttctgcgg ccttaagata gtgaagtatg cctttggtat atagaagtct agcaacaagt
    19981 atgaatttca cacatgatct ttgctttaaa ttcttatttg agggaagaaa ttttgataaa
    20041 tcaacaccag acccaaatat tcggcatgtt ttctcataat tgacgcagcc agaattaatc
    20101 aataactgac gatcatcctc gttttgaaaa aaaacgaatg ctgctttctt taatgctatc
    20161 ttatataata gcttaacaat ttgacttaat atcgtattac tattaacaaa tgcacttcca
    20221 aggccagaaa tattaggtat cactttcgcg tcaaatttat aagcaagcaa accacaatat
    20281 atattagctt tgggtgtaaa cgttaatata acctgtggat tgaatttttt tataatttta
    20341 taatatgata ttagagatgt acattcagta aaaatattaa tagattttgg tttaagatca
    20401 accttctcaa atttagcgcc gaattttaaa atagaatcct tatattcata atccggtaca
    20461 accactaata catcaaaccc tatattaata agtgccttaa ttgtgcttcg tctaaaatta
    20521 tatatatacc aactactatt agcagttaaa actattttta aggaatgatt atcccttaaa
    20581 tgatcctttt cgtccaagca gtccatcttt tatcccctta aaaatataat ttaatcgttg
    20641 tcctccacgt tcacaaaaag cgtaaataat gattcggaca ggcattgtta tcaatttttt
    20701 taacttatat cttaaaggaa atacaggctc tctcagagct aataaccaat ttctataaac
    20761 ataatacaaa cgaaatggac tgcaaactgt aaatgtttta atccccataa tatttatatc
    20821 tttagatcct atattgtgag aaataaggac tttatctgtc actaggatat tccagccttc
    20881 tttcctagct ctgaaactcc attctatgtc aattaaatcg ataaaccaag atactttatt
    20941 taatccaata ttgataattg cctctagggg tatgagtgca ccagaagata ttatactttc
    21001 aacatcatgt atcgtttcac ttatctggga gcccagcttt atttcacgat catacttttt
    21061 cccattacgt tcatttatta ttgttggacc taatgcagca atattactcc ggagagagca
    21121 agcgtactca tactctttta acattgatga tatataatct tttgatatac atgaatcttg
    21181 atcaaatgta aagaataagg actttcctaa tccgatagct ttaatgaaac catagttctg
    21241 cgcaaacgca atgcctttgt tatctcctaa atgatagtaa tgaatgttgt tattacacaa
    21301 aacatcactt tctaagtgga gctcaggtgt attgtcaaca agtaatattt cgcactgttt
    21361 ttttcccagt gagtcaatca ttttattgat tttactaaca tcaggataat atagtacaat
    21421 tatcaccgct actctattca ttttttattc cacttataag atccatgatg agtccttttt
    21481 acaaaatttt tatcggatac tttaaaggtt atgtatcgat aaaacaaaaa atatcccgac
    21541 aataatacaa tcttccagtc ctgatatata tatgcataca caatagcagc atcaaaaaag
    21601 aaaaagtaat atttaaccct aaataaatca gggtaaataa taaaatcctt taacctttta
    21661 ataccatatt tctccttaca ggcgtgttct aatgttctga ttaaagggaa taataataat
    21721 atgataaaaa caaaaaatac actgcatgtg acagtgggta tcaaaggaat aatgtaacga
    21781 catgacacca aagaaaaata agtgattata tttctcttat cccggattgt attatgcaaa
    21841 taaaaggaga taattaaaaa ccctatacat aatacaaaat agataacagt atagtgatga
    21901 gcaatgatat cactttgata aattaataac gcgaatgata caaaaaaata aacaactcta
    21961 atgaaaatta tacttgataa tttactttta attgtatcac gcagctctcc acctcttatt
    22021 gttggtgctt tttctgtatt tattgttcta tagtcattgt ctaagtagcc tatttcataa
    22081 atggacatgg tcatcataaa tgacagaagc aaagatacaa aactaccatt atacaaaaaa
    22141 caacacagca atagaaaccc tggatatata ataaaccagc taattttttc atgccatgtt
    22201 ttcaatcttg aaatgaataa atatgataat gggaaataca atatcacata aacctcactt
    22261 tatctttgga gacaccaaat ctatttaata gtactctcca tttattttta tctttggctt
    22321 ttgaaataat cactgctgta tctgcaagtt taataagatt aatatcactg aaattatcag
    22381 tgacaatcaa acttacaggg ttattaactt gtaaatgctt tataccagta agattatatt
    22441 ctaataaccc tgttgattta ttgtcgataa attctaattt agatgatact gcctcaacat
    22501 tcaaattttt agcaattaca tttatgacag gatcaacgga agcagacatt aaaatgatct
    22561 tctcatgttt atatcgttct aaaagtgcca taacttcatt cttttcttta aaaatgagaa
    22621 attcatcata aaacaaattc gccaactctt ccaaagtaga cttttcatat ccttctaatg
    22681 tcttcaaaaa atgttttcgt aaggaacata tacgcaattt atgtagcaac tttccaaata
    22741 aggagcgtaa taataagcat ttaattaact tggtatattt tttttgtttc ctatgtacaa
    22801 atacaacata atcaaaagtt gtattcgaca aataaagagt gccacataca tcaaatacaa
    22861 caatatgttc gagcatgtta aactcccttc aaagttagag ttaatcttga gcaaaaacat
    22921 atcacataaa gcattgcaat tgcataaata tagaacatca acatatataa ttggtcaaaa
    22981 aattgaaata acaaggggaa gtacaaaata gagcaaatga caactgatat cgttttctta
    23041 tttttttttg ctacattgta caaagatgaa tgaaagaaac ctatgacaaa aaagaaaatt
    23101 aatgagtaca aaaaatcaga atattttgtt agcgaataaa atattgtaaa aacatttgtc
    23161 ttatactctt tatatgatat aaaattagct aatccacatg tagcatctcc attacaatct
    23221 ataactactg ctttaccttg caaaaccgaa tctaatgcaa aaatgtttgc caagctatag
    23281 ttcatcaatc catattttaa cgcttcgaat gctcctgaag agtcgtcaat tttatttaaa
    23341 aacaacatag ataaatagaa aaaaaatata ataccagaca ctgtgacaag taaatatttt
    23401 aaagatattt ttgaaaaaaa taatcgcaaa aacactattg gtataattag taataaaact
    23461 ttaattttag ctgttgataa taacgcataa aaaagtgaag taaaaaaaac aaaataaaat
    23521 gttcgattat tatatggtaa ccgggcccgc tctccttctt caatcttttt atatttattt
    23581 ttaaaatata aaaagctcca tacatatacc aatgggtata aaagatagta tatataccca
    23641 tatattttta ttaatcccga tttatctccg taattaattt ctgtacgtga atttatatag
    23701 gattcagtga agcccccctt ataataatat aaaaaaacta ttaaagtata gattaaaata
    23761 acaaacgaga taatatttgt aattaatact aacgcttttg tattgtttat attataggaa
    23821 aatacatgtt caaatatagg agcgtaaggt tttgccagta gagcccctac cgaaaaagat
    23881 aaacatgaaa ataaaattat acataatgta tagttagtta tgtctccatt tactcccgac
    23941 aaaaataaca gcccccccca gataaagaat agaatcgccg acggcgattt aatatcttta
    24001 ttaatactca gtaataatcc tgaatacata agcattatta tagctataaa aatggtcatg
    24061 ttcatttaag cttagctcta agttgagaaa ttaattgagc tcctcttcca ctgcattcat
    24121 cagtgttaat tacaaattcc tcaccgactt taaaaacttc tttattttta ttaatccata
    24181 cattcagaag tcgctcagca ataaagccga acacacgtga ttgatattca tcgtatctgc
    24241 ataaatttat ttctttctcc agctcatcta atatggaaaa caaccaatca caataacaat
    24301 caaaaatccc agccttcatt ataaacatat tataaagcga tatttttttt tgttctaaaa
    24361 gttctctgaa tgcaacacta taatcaggat ataatttatc aactattttt tttgccaaca
    24421 ttagatcttc gacattatgg aatttcctgt agtgttcaac aacagatagc ttcaaactct
    24481 ctttcaccgg aataattata tcatattgtt tcattttatt agaaatatta gctctgtcat
    24541 atatcctcac ctctttaaat ttaaaattcg ataaaacatt tccaaaatac ctcctataat
    24601 gaaccaagcc aatataatca tcattaaaaa gtttattttt ccaaagccaa tatataacgg
    24661 tcagctcaca ataattttta tttttacatg atatattatt ccctgtatca tctcccaaca
    24721 cttgatcaag cattttccct gatattttct tacccgcatg aatagggata tagcactcat
    24781 gattaggtaa ttcagtttgt ttatggcaag caatgaataa agccattaat attcctcttt
    24841 atttaaacaa atttattttc tttcttttta agaaaagata catcaatgat gcaactacgg
    24901 tttctgtaat taaaaccgtc atcgcagccc catacgcccc ggcaaaaaag atcaaaggag
    24961 caatgattat cacatgcata acaccacaaa ttagcagtat tttactaaat tgtcttttat
    25021 aaccatgtgt cagcatggtt tgaatgccaa aaaaattact aagagaaata ataaatggca
    25081 aaaaggctaa tattcttaaa atactaattg actcagtata tttatctccg actccatatc
    25141 ttattattat tggagaaagt atgcacagga gtaatgaacc tgaaaacgaa atgaataata
    25201 cgcccttaaa tgattttttc agaagattca tactttttgc ataattttct tcaaacaatg
    25261 aattaattct tggataaaca gcttgataaa taggattcat tagactctgg gctgcatttc
    25321 taattgtatt agcagcgtta aagtagccaa cagagacagg tccacatata aacccaagaa
    25381 caacaacagt tgttgttgta tataagctaa tagcagctgt tgaaatgaaa atatgccatc
    25441 cgctttttaa tgaataaatt ataccatcat aatcaaatac caatttccca atccagttat
    25501 tatttataat aatagcaaat ccaattaaac ctgctataaa attaacgccg ccttgaatca
    25561 aagcggctaa ccagatatct tgtggatttt ttactaacaa aaaagttaat ggtattaata
    25621 ataatcttgc acttaaacta cataatgtaa tccatttcat tttttctaat ccctggaaaa
    25681 accaaacagg gtataatata ttacctaaca ccattggaat aaatgatatt aacaccactt
    25741 gagcataata tagctcctta ataaaaaatg ttattccgaa aaatataaca attgccgaaa
    25801 gtgcaagtat aatcttacta aagaagacat tccaaaatat tgttgttaat ttttccttgt
    25861 cattcttact tttcgaaaca tcttgtgttg ctgaccaatt aaacccataa tcagtcacca
    25921 tcacaagata ttgcattgtt gcttgacaaa accctaatag accaaaagag tatggtccta
    25981 atactctaac aagataagga aatgttaaca atggcagtat aaaataactt ccttgcaata
    26041 tagctaaata gaagatattt ttattaagag aggtaacaag cttcaatgca atctccgagc
    26101 ctatttatat agctgcaact agttaatatt atgatatttt attatttatg atatttagca
    26161 gatactgacc ataagcattt tttttcagtg gttcagctaa tattttcacc tgctcagaat
    26221 caataaaccc tttacggtaa gcaatttctt ccgggcagga aactttcaac ccctgacgtt
    26281 cttcaattgt agcaatgaag ttgcttgcct caatcaggct ttgatgtgtc cccgtgtcca
    26341 gccacgcata accacgcccc atcatggcaa cggataaacg cccctgttcc atataaatac
    26401 ggttaatatc ggtaatttcc agttcaccac gggcagaagg cttaaggttt ttcgccattt
    26461 cgacaacgtc gttatcatag aaataaagcc cggttaccgc ataattactt tttggttgta
    26521 gcggtttttc ttccaggctt attgccgtac cgtttttatc aaactcaacg acaccgtagc
    26581 gttcaggatc attaacgtga taggcaaata ccgttgcacc actttctttg tcaacagcgg
    26641 tatccattaa cttcggcaga tcgtggccgt agaagatatt atcaccaaga accaaagcac
    26701 aatcatcacc accgataaac tcttcaccga taataaacgc ctgcgcaagc ccatctggag
    26761 tcggttgcac tttgtactga agatttagcc cccactggct accgtcaccc agcagttgtt
    26821 gaaaacgagg agtatcctgt ggcgtactaa taatcagaat atcgcgaata cccgccaaca
    26881 tcagtgtaga gagcgggtaa tagatcatcg gcttatcata aataggtaat agctgtttac
    26941 tgacagccat agtcacagga taaagacgtg taccagaacc acccgctaaa ataatacctt
    27001 tacgcgtttt catttcatca ttccttttaa ttcatcttgc tccatcatca cgaacaagat
    27061 gcaaaaacta ttaaattgct gtagtcgtaa ataattcgtt gagcattcgt ttcacgccaa
    27121 cctgccagtc aggcaaaaca agcgcaaagt tctgctgaaa tttttctgta ttaaggcgag
    27181 agttatgtgg acgacgagct ggtgtaggat aggctgttgc tggtactgcg ttgagcttgt
    27241 tgagtgcaag gggaatacct gctttgcgcg cctcttcaaa aaccagcgca gcataatcgt
    27301 gccaggttgt ggtaccactg gctaccagat ggtacatgcc tgcgacttct ggtttattca
    27361 gtgccacacg aatagcatgt gccgtacaat ctgccagcag ctcagcacct gttggcgcac
    27421 caaattgatc attaatcaca gccagttctt cgcgctcttt tgccagacgc aacatcgttt
    27481 tggcgaagtt atttccttta cctgcgtata cccagctggt acggaaaata agatgcttcg
    27541 cgcaatgttc ctgtaacgct ttttctccgg ctaacttggt ttcaccgtaa acatttagcg
    27601 gtgcggttgc atccgtctcc agccatggta tttcaccggt tcccggaaat acgtagtcag
    27661 ttgagtaatg aattacccag gccccaactt cattagccgc tttggcaatt gattcaacgc
    27721 tggtcgcatt gagtaattgt gcaaattcgg gttctgactc agccttatct actgcggtat
    27781 gagccgcagc attaacaatt acatcagggc gaattttttt gacggtttca gccacacctt
    27841 caggattact aaaatcacca caataatcag tggagtgaac atcaagagca atcaaattac
    27901 ccaaaggtgc gagagcacgc tgtagttccc aacctacctg ccctgttttg ccgaaaagga
    27961 gaatattcat tactggcggc cctcatagtt ctgttcaatc cacgattgat aagcaccact
    28021 tttcacattc tcgacccatt ttgcattagc caggtaccat tccactgttt tacgaatccc
    28081 gctctcaaac gtttcctgcg gtttccagcc caattcgcgg ctaatcttct ctgcatcaat
    28141 cgcataacgg cgatcgtgtc ccgggcgatc ggcaacataa gtaatttgct cacggtaaga
    28201 tttctctttc ggtacaatct catccaacaa atcacaaata gtgagcacta catcgatgtt
    28261 tttcttttcg ttgtgtccac caatgttata agtttcaccc gctttacctt cggttacgac
    28321 gatatataac gcacgcgcat gatcttcaac atacagccag tcacgaattt gatccccttt
    28381 gccataaata ggtaatgcct taccttccag agcattaaga attactagtg gaatcaattt
    28441 ttccggaaag tgataaggac cgtagttatt cgaacagtta gtcacaatgg tcggtaaacc
    28501 ataggtacgt ttccacgcac ggactaaatg atcgctggat gcttttgatg cggaataagg
    28561 actactaggc gcgtaagctg tcgtctcagt aaagaggggt aattgttctt tattatttac
    28621 ttcgtcagga tgaggcaaat caccatagac ttcgtcagta gaaatatgat gaaaacggaa
    28681 gctatttttc ttgtcgccat caagagcaga ccagtaattg cgagccgctt ccaaaaggac
    28741 ataagtacca acaatattgg tttcaataaa tgccgcaggg cctgtaattg aacgatccac
    28801 atggctttca gcagccaggt gcatcactgc atccggctga tgctgagcaa aaatccgcgc
    28861 cattgcagca gcatcgcaaa tatcagcatg ttcaaaaaca tagcgttcag agtcagaaac
    28921 atcagcaagt gattccaggt ttccggcgta cgttaattta tcgacattaa caacactatc
    28981 ctgcgtatta tttataatgt gacgaactac agcagaacca ataaatcctg cgccaccagt
    29041 aacaagtatc ttcactttat tattccatat tacttcagag catgctgtga aataagcggc
    29101 tctcagtttg attaatagag gtattaatgc acgctaccgc ccctggcttt acagctacca
    29161 gagcactgca tgcatgtcta cgatgtgacg agcgttaccc actcgcgcta aacccgaaaa
    29221 attcaaacgc taattgtctt accaatccgc tctggaaaca aggaaaatcc tggaaaactt
    29281 tgactaaaat cctattgcta actcgttgtt atcctgattg tttatataaa acaacggcag
    29341 gaatattcgc aacaaattac tttcgccacg aatcttcact gccgttataa ttttcttatc
    29401 aaccgttaca tccggtcaga ttttcattat tcgcttaaca acttctcaat ccctttacgg
    29461 aacttcgccc cttctttcag gttgcgtagt ccatacttca cgaacgcttg catatagccc
    29521 atttttttac cgcagtcgta gctgtcacct gtcatcagca tggcatcaac ggactgtttt
    29581 ttcgccagtt cagcaatggc atcagtcagc tgaatacgtc cccatgcacc aggctgagta
    29641 cgttccagtt ccggccaaat atcggcagaa agcacatagc gaccaacggc catgatgtct
    29701 gagtccagcg tctgcggctg atccggtttt tcgataaatt cgacgatgcg gctgacttta
    29761 ccttcgcgat ccagtggttc tttggtctgg atgaccgagt attcagagag gtcacccggc
    29821 atacgttttg ccagcacctg actgcggccc gtttcattga aacgtgcaat catggcagca
    29881 aggttgtaac gcagcgggtc ggcgctggcg tcgtcgatca caacgtctgg cagcaccacg
    29941 acaaatggat tgtcaccaat ggcaggtcgt gcacataaaa tggagtggcc caaacctaaa
    30001 ggttcgccct gacgcacgtt cataatggtc acgcccggcg gacagatgga ctgcacttcc
    30061 gcaagcagtt gacgctttac gcgctgttca aggagagatt ctaattcata agaggtgtcg
    30121 aagtggtttt cgaccgcgtt cttggacgcg tgagttacca ggaggatttc tttgatccct
    30181 gcagccacaa tctcgtcaac aatgtactga atcattggct tgtcgacgat cggtagcatc
    30241 tctttgggaa tcgccttagt ggcaggcaac atatgcatcc caagacccgc tacaggaata
    30301 actgctttta aattcgtcat tatttcatcc acctgtaaaa tggttgctga attatagctt
    30361 gttcgatttt tttcgccagc atcaattacc ctgaattgat tactgaatta cttgtgatgt
    30421 tacgccgctt cgttgtggat tgcagtagca ttgttcctaa gtatggctcc atttttccag
    30481 gaatggtcgc aaatctactc cccccgttcc ggcaatctaa agttaatctt ttccacatta
    30541 acaatatggt gattaatcct gtcgatatcg acggagcttt gccctctttc attcaccgca
    30601 tgaacatttg caagagatag tagtgtttct tttttcgcca taaagacgcc gcgaacgtct
    30661 ttgcgcatgt cgaagttcat gctcaatgct ggtccaacag aggattcctg catcacatta
    30721 atattacgca taaaaaggtg ttgtggtttg ttgtgtaact ccagcgacgc acgcttcatc
    30781 tcaatgttag tcagcgccac acaggagaca gcattcccgg cggagatttg gatgccgcgc
    30841 aatttataag caagatgggt gttatctagt tgaatattat tcactcggaa attttgcggt
    30901 atcgagagat atttgccttt aattacccca tagccgatta acatcccggc gctattaatc
    30961 atttcaatat tatcaatcac gaaattgtca caaccgtaaa tagcaactgt cgcgttatca
    31021 atacctgctt tcttactgaa atccggcgtg atattgcggg ctttgatatt acgaataaca
    31081 aaatgtttac cattttcaac atggatcagt tgccgacaat ccgatcccgt gatattcgcc
    31141 acgacaaagt ttttcactgc ctggtcttcc ggataattat tatcgtaggt acttcccgca
    31201 agacctatgc cgatgcccca gttgattttg ccgttggtac agttgatgcg ctcaataacg
    31261 tggtcagata tcaaaatatc actgtcgtta atcgccacgt tccattcaat ggcatcgccc
    31321 tgtaagtcgc tgaacttaca attggtgatg ttggcaccga taatctggtt atgaaatccc
    31381 tggcgtaaga tggcgtaatt agcgtggcta acggtcaggt tatcgatggt caggttgcgc
    31441 atgacctgtt tgtttttgcc gccgatataa atctgcgtta ccgggccaaa gccgctcata
    31501 gtcagccctt tgatggtgca gtcggaacca cgcacatcca gggtgatgtt atgcatactg
    31561 ccgccctcct cccctgtcac ctggctgccg tcctgtaaaa caaatcgccc tctgccgttg
    31621 ccgcgcaggc ttccaaggat gtgtaacgtt ttacctgcag gaatgaagat gccggtgttg
    31681 atattgtcgc aaaccaatcc gccaggcacg acgaccgttt gcccttcgct gaaggcttgt
    31741 ttaaatgagg cgatccagtc gtgagggttg tagtcgttaa tgttaacgct ttgtcgggcg
    31801 ggaagtgcgc gagcgaaagg ggtatggagg aaggcaagcg ccgagcttgc cgtcaggaag
    31861 gtgcgtcggg agagtttttt aaatggcatg cgttctcctc tataaagcct gcaacaagct
    31921 ggcgagttct cgattaatca cctgctggtt aaaatcgtgt tcgacttttt cgcgcgcacg
    31981 tttgacaacc gtagtaagtt cgtcggtgtc cagttggcta aacgccgcca gtcgttgcgc
    32041 cagtgcgcga gcatcgttct caggcaccag ccagccggat ttatcggcct ccaccagttc
    32101 cggtattccg ctatgcagag tagaaaccac cggaatgccg accgccatcg cttccatcag
    32161 cgccaccgga atgccttcca tatcgccgtc agcacccgtt accgatggca acagaaagac
    32221 atccgcatcg tcgagcatcg ccttcacttc gtggcttggt ttaaagcccg gcatctctac
    32281 cacatcctcc agttgatatt gttcgatgag cgtgcgcagg cgtcgttccc acgggccaat
    32341 gccgaggatg cgataacgaa atgccacgcc ctgctctttc aactgccggc aggcttcgat
    32401 cgccacatgc aggccttttt tctcggttaa gcgtgcgaca gatatgattt ccagcggcgt
    32461 tgcaggcgct tttaccggac gcgggctaaa gcgcgtcatg tctacaccca tgcgcgatac
    32521 ggcaattttt tccctcgggc agcccatttt ttgcagcctt ccggcccaca gttcgcttat
    32581 cggtagcatc aaatcgccac gacgaaacag ttgttgatat tcgggagtgt aatggttgag
    32641 cacttcccga ctggagatat caataccgtg gaagatagtg gcaattttgc cgcgaatgac
    32701 atccagttcg cgtagttttg ctgcggttac ccccgcaggg ccaaaatgag cgataaaaac
    32761 atcagcatga aacggtgttg cgacctggcc gcaaatggca gacaaaatca ggttccgcga
    32821 ctcagcacca tagcgtttga ggttgagcgc ctgccaggta tttttacgat gaatgccgcg
    32881 taaggtctgg ctggcgcggt agcgcagttt cgccactttg ccttgtggtt cgtcctgtaa
    32941 ccagcgagtt ctggcggcaa ggttatattt cgtccatgcc gcgtgggtgt tttgcgtatc
    33001 gcctttttgc agcgcgacaa tctccacctc aaagcccata tcaataaacg cagtgatttg
    33061 gttgagaacg aaggtttccg acgacagcgg aaatttcaat aagaagaagc cgaccttcat
    33121 ttcgcctccc cgatgcgctc aagcacagac tgcaccatcc gcattcccgt ctgacgctca
    33181 cgactgacgg cttcgttaag tcgcgcgttc agcgccggaa gttggcctaa ggtatccgca
    33241 accatcgctt gcaggctgcc gtccagtaaa tggcgaatat caatcgccat ctctggcagc
    33301 cccagttgtt gcataatccc ggcggatttg tgttcgtagt tgatggcaat tgccggagtg
    33361 gagaaattca tcgagataat ggcagagtgc aggcgcgtac ctacggtgag ttcacaggca
    33421 ccgaggattt tgcccatttc cagatcgttg agctcatcca tcaccacgtg ataacgggca
    33481 ggatcgctga tgtgctgacg caggttgagc gccaccatgc ggtcgtcttt gttatagctg
    33541 tcgatacccg tacaggtgga gagcgcaatc acctgatagc cttcgtcgag aatgcgattg
    33601 accaccccgg caaaggcttt ttcatacgct tcttgagtgg tgccgagacg tttgtcgaac
    33661 ggtgccagtt cgcgcagggt aattgccacc gctttctgtt gtgcggcaac atccagccag
    33721 tgttgaacag catagctggc ggtgaagtct tcaatgtggt gatcgaccag ccacgcggta
    33781 tcgacgccat tttcaacttt tgcggtagtg atattgctgc gtttcattag atccaggctg
    33841 accgattcgc gcaggatcag cgcgtcgcag tgaccgaata cgtagttcgc cagttggtta
    33901 aattgctcat cctggaacgg gccgacgctg tgaccaatca taaacagcgg ctttttcgcc
    33961 ataaacgtgc aaagcgcatg ttcaaactgc ggcacgccgt agagatcgac aaaaaacgat
    34021 ccgccgacct ggataatggc atcgtaacct gacagcaggc gcacaaagtc agtgaatccc
    34081 tgggcaatgg cgatattacg cagcttgccg gtgtcggtga cgcgtgagag caatacctgg
    34141 tgctggtagc ggcgacggag gacttttttt acgcgcccga caacgcccgc cgcgctgttg
    34201 tgttgtttca tttgcaggaa cagcggatcg cccattaccg gacggttgag cagccaggaa
    34261 gaactgaccg gatagcggct cattacgtcc acttcggcgt gtggattgag gatgttgatg
    34321 gcatcaagta agccgcgcaa gatggcgctg tcgccgcgat tgccgcaagt gtggttgccc
    34381 agaatcagta atttcatttt ttcctcataa atttgatgcc aagtgaggct gtgttttttg
    34441 tgttcgttgc cgctttttgc ctggcgcgtc ttatcaggcc tacaagtccc gagcacagag
    34501 ccgtaggccg gataaggcgt tcacgccgca tccggcaact attgccggaa ccgaaaacaa
    34561 ccattcaccc ggcccgcaaa agcattttca ttttttcgct gcgacaaaac tgacgcttca
    34621 cttccaccac cagcggatgg cgcgacaaca cgatcatcac cacaaacgcc agcacgcctg
    34681 cgataatttg caccgccagc agcatcccca gcgccagttg ccctttcagc acaatgccca
    34741 gcgcataact gaccaccagc gtcggcaacg agagataaaa cggcagccac aaactcagga
    34801 tgtactggcg ataactggaa ccaagaaccg gtttaatcat cacgaagtaa ctcagaatgg
    34861 tgttgataat ttgcaccagc aggaagccaa gcgtgacgcc gatcgcgccc gccatctgcc
    34921 caccaataac aatcgccgga ataaacagaa atgttttgaa tacgttgaat ttaaagctga
    34981 tatcgacccg cgctttcgcc atcagcagcg aaccaatcgg gttacccacg gagcgcagca
    35041 gacccacaat acacagcaat tgcagcaccg gaataatgct gttccacttt tcgccgaata
    35101 ccagcggtac gaagttattc gacaccacca tcagcccgag cagcgccgga aagttgataa
    35161 tccccaccac cgacagcagc ttgtagaagt tcacgcgcag cttttcggtg tcgtcctgaa
    35221 ttttggcaaa cgccggaaac aacacacggg tgatgattgg gttcagcttc atcggcggca
    35281 caacggccac gttgtacgcc aggttgtatc cccctgccac gcccgcgcca agaatacgcg
    35341 ccagcaccag cgttgaaagg ttggtgttga gatagttgat gatgctgtcc gccgtcagcc
    35401 aggcaccaaa acgtaagttc ggcgcaactg acgccagcga gaaatgcaga ccggggcgat
    35461 aaatttttcg accaaagtag ccaaacagca gcgttcttac cgcactattg accagataac
    35521 cgaggatcgc ggtcatcgcc agcggccaga aatgggcgct aaccaccgta aaagtgaagc
    35581 ccgccagcac cgcgctggtt tcgatcatgc cgattttgtt gaactccagt tccttttgca
    35641 tcaacgcgcg gaactgttgc ccgtggggga tcaccacaaa cgccagcgac aatgttttaa
    35701 tcaacggtgc caggtccggg ttattcagca cgtcaccaat ggcatcactc aacaaaaaca
    35761 ccgccacaca caccacgatc cccagcccga cgttcagcca gtacagcgtg gtgagttcca
    35821 ggtggctgat ttcttttcgc tgaataatcg agttagcgat accgaagtca gaaagcgtat
    35881 ctgccagcgc gatgatcacc agcgacacgg taagcaaacc gaactggtgg ttgtcgataa
    35941 tccgcgccag cacggtcatc tgcaccagcc cgaggccgat gatgatcacc gtagcaatcg
    36001 ccgaccactt tgcgccgctg atggtctttt cacgtaagct catatcaata tgccgctttg
    36061 ttaacgaagc ccttgaatac cgtcaggaaa acgattttga tatcgaacca gacgctccat
    36121 tcgcggatgt actcaaggtc gaactcgacg cgtttttcca ttttctccag cgtgtcggtt
    36181 tcgccacgcc agccattaat ctgcgcccag ccggtaatgc ccggtttcac cttatggcgc
    36241 agcatgtagc cttcaatgag ctggcgatac tgttcgttat gcgccaccgc gtgtggacgt
    36301 ggaccgacaa tcgacatccc cccggtcagc acattgataa actgcggcaa ttcatccagc
    36361 gaggtgcggc gcagaaagtt ccccactttg gtgacgcgcg gatcgttctg cgtcgcctgg
    36421 gtcaccactt tgtcgttctc catcactttc atggaacgaa acttccacac tttgatcggc
    36481 ttgccatcca tgccatagcg agtctggcgg aaaataaccg gccctggtga actgagtttc
    36541 accgccagcg caatacagca cagcaccggg gagatcagca acagaataag cgtcgccagc
    36601 acaatgtctt ccgcacgttt gagcaggcgg ttaaccccgg aaagcggcgt gtcgtaaagc
    36661 ggcaccaccg gaacgccgtt catctcttcg agacgtgaat ggagaatgtt gaaggtaaag
    36721 acatcgggga tcagcagcac cgaacaggtg gtatccgcca gttgatggac cagttttttc
    36781 actcgcgcgc cgtcgcacat ctgcatcgcg atatagacgt tatgaatttt gcccgctttc
    36841 gcatcctcga ccagctgttg cagattgccc gcccagtcgt tagaaacgcc gcccggtttc
    36901 gggtcgtggt aaacccccac cacttcaaac cctaaccacg gctgattacg gaagctctcc
    36961 atcagcattt gtccggcggc taaatctccc gctacggcga ccatacgctt gttatagcca
    37021 tgattacgca gccagcccgc cccaatgcga atacatgaac ggcaaaccac cagtccgata
    37081 ctggtcagcc cataccacgc cagccagatt ttcagttgcg tgtcgaaatc attgttgaac
    37141 gccaccagtc cggcgctgaa aatcacgctt aaggtccagt tttgtagcaa cagggcaaat
    37201 tctgtcgctg cccgaacacc gcgccatgag cgataaaaat cggtgatgcc gcccagcatc
    37261 tggaacacca ccagcgtaat caacgccacc aacaggtgca tgtagaggaa tgacagtccg
    37321 ctcacttcgc aaaccagcca tagtccggca aacatgatgg tgatatctga aaagcgttgc
    37381 accatagaga ttaacgatgc attggttttc gctcgctcgc gcttttttag atttgtcatc
    37441 gttgttcctg ttattagccc cttacccgca gtggggtaag ggaagatccg acattactca
    37501 ttcagcaacg tcagcagagt tcgcgttcgc gcttccatca gcggcacatc accgcgcgat
    37561 tccacattca accgcaccac cggttcggtg ttggaggtgc gcaggttaaa gcgccagtcg
    37621 gcaaaggtca tgctgatgcc atcggtacga tccaccgaca gcgcctcacg gctaaaatgc
    37681 tgttcgacgc gattaatcgc ctcaacgggt tgcgccagtt tgctgttgat ctcaccgctt
    37741 gccggaaacg ccgccatccg gtcgcgcacc agttcgccca gcgtttttcc tttcaggcac
    37801 accagttcgg ctaccagcag ccacgggatc atgccgctgt cgcagtaagc gaaatcacgg
    37861 aagtaatggt gagcgctcat ttcgccaccg tagatggcgt cttccttgcg catccgttct
    37921 ttaataaagg cgtgtccggt tttcgacatt accggggtgc cgcctgcggc agtcaccaca
    37981 tcaatagtgt tccaggagag acgcggatca tggatgatct tcgcgccggg atttttttcg
    38041 aggaacgctt ctgccagcag gccgacaatg tagtagccct cgataaactg ccctttttcg
    38101 tcaaacagaa aacagcggtc aaaatcgcca tcaaaggcaa tgcccatatc cgcgccgtgt
    38161 ttgatgaccg cattgcgggt gtcgtcgcga cattccggca gcagcgggtt aggaataccg
    38221 ttggggaaat tgccgtccgg tgtgttgtgt actttgatta attccacggg cgcgccgagg
    38281 gctttaaagc gggcttcaat ggcgtctacc accggacccg ctgcgccatt cccggagtta
    38341 atcaccagct tgagcggcgt gaggtttttg acattgatat aaccgaacag gtgatcaacg
    38401 taagcgtcac gcaggttgat ttgctgatag cggccgcgtt tggtttcatc gacgggagga
    38461 aagtcgttgg cttcagccag acgctggacg tcgcgcagtc cggtatcacc gctgatcggg
    38521 cgagcccctt cgcgcaccag tttcatgccg ttataatcca tcgggttatg gctggcggta
    38581 acttcaatgc cgccatccac gccgagatgg aacgtggcga aatagatctc ttcggtgccg
    38641 gacatgccaa tatccagcac atcgacgccc gcatcctgta aacctctcgc cagcgccagt
    38701 tttaaggttt cgctggtgag gcggacatca ccgcctaaca caatggtttt cggtttgaga
    38761 aattcgccat aggcgcgacc aatgcgccag gcgatatctt cattcagttc ttcgcctagt
    38821 tttccgcgaa tatcataggc tttaaagcag gttaattttt tcatatcgtt accctttttc
    38881 aggcaatagt tggccctgac cgaagcggcc aattttattt ttgtcgttat ttacaccgcg
    38941 gtttcgcatt cattgcctga tgcgacgctg ccgcgtctta tcaggactac aaacccgaac
    39001 cgtaggtcgg ataaggcgtt cacgccgcat ccgacaaaca gctcctgatg cgacgtttac
    39061 acccgtccgt agcgatccgc gaagcgcacc acatcatcct cttcgagata agagccggag
    39121 cgcacttcaa ttaaatcgag cggaattttc cccgggtttt ccaggcagtg cgtcgccccc
    39181 agcggaatat aaatggactc gttttcacca agcagtttga tatcaccatc aatggtgact
    39241 tttgccgttc ccgcgaccac tacccagtgt tccgcgcggt gatggtgcat ctgtaccgac
    39301 aagccctcgc ccggtttcac ggtgatgcgt ttcacctggt agcggtcgcc cgcgtcgata
    39361 gagtcatatt tgccccacgg acgatacact tcgcgatgca cccgatgctc atggcgacca
    39421 tcggctttga tctgctcgac cacttttttc acatcctgca ccgcgttacg gtcggcaatc
    39481 agcaccgcat ctttggtctg caccactacc agatctttca ccccgacggt ggtgaccagg
    39541 ccagattcgg cgtacacata gctgttttca gttttgtgat taatcacatc accgtggcaa
    39601 acgttgccct cggcggtgtg ggcgctgatc tcccataatg aagaccagga accgacatcg
    39661 ctccagcccg catccatcgg caccacaacg gcatctgccg tacgttccat gaccgcgtaa
    39721 tccaccgact cttccgggca ggcgagaaac gcttcttcat ccacgcgaat aaaatcgaga
    39781 tccggatcga cggcgctcat cgcttgttca caggcgtcga gaatatccgg gcgatatttt
    39841 ttcagttctt cgagatagcg tccggcgcgg aacaggaaca tgccgctgtt ccagtaatat
    39901 tcgccgcttg ccacataggc ctgggcggtt tccagattcg gtttttcgac aaactgcgcc
    39961 acttcaaagg ccaccgtatc ctgctcaccc gcagatactt caccgcgacg aatatagcca
    40021 tatccggttt caggtagatc cggcacaatg ccgaaggtca ccagcttgcc cgcttcggca
    40081 tacggcatgg cattacgcac ggcggcacgg aacgcgtctt catcggcaat cacatgatcc
    40141 gccgccaata ccagcattaa cgggtcgctc tccgggctat gacgttttgc cgccagcgcc
    40201 gccagtgcaa tggcaggtgc agtgttacgc cctgccggtt cgagaataat gttctcggtg
    40261 agtttgttca gttgacgcag ctgttccgcg acaataaagc ggtgctgctc attgcaaatc
    40321 accaccgggc tttcgcactc cacgccgttc aggcggcaga tggtggtttg cagcatggtg
    40381 agatcgcctt tcaggcataa aaactgcttg gggtaaagta cgcgggaaag cggccataag
    40441 cggctaccgg agccacctgc catcacaact ggatagagtt tcgactgcgc cataattatc
    40501 cccgaatatc atttataaat tgacgtaaca cgttctcttt atcgagtgtg cgttcggcat
    40561 attcacgtgc caccgtgttg tgtttgggca gcaggagcgc ctgacggatc cccgccacca
    40621 gcgcctcgac cgattccggt tctacgcaaa ccgcaatgcc cggaaaggtt tcgcaaagct
    40681 gccccagttc tgtgtgggcc tctgcggtaa tcaccgcgtt accgcctacc gccagaatat
    40741 tggtcagttt cgacggcaat acggcatctg ccgcgccgcg tttttgcacc accagatggc
    40801 aatcgcccat cttcagcagt gcgggtaaag cgtcatacga ttgcagcggg aaaaattgca
    40861 tgttgcgcag tccacgctgc tgcgccattt tttccagccg cgctttgccg ccgccctgcc
    40921 cgacaatggc aaaaatcagc ggttcatcgc gcagacgatc ggcagcttca ataacgtttt
    40981 ccagcccctg cttttcacca atattgccgg agtaaagaat gatttttttg ttatccggca
    41041 ggtcaagctg gttacgaagg gcgtcaacat cggcatctgc aacatgctga aaacgggcaa
    41101 tttccgacca gttggggaag aagatgacgt tttccgccgc cacgcctttt tcgatggctt
    41161 tattcatcat cgaacgcgaa atcgtggaga cgttatcgac gttatgcagt ccgctacgtt
    41221 cgaacgccgt tgccagctgt gccactttgc cgcctttgcc ttttccggca aggcccagcc
    41281 ccagcatggc gtccacttcg taatcctgaa tatgcagcac ggtacgcgca ccagagagct
    41341 tcgccagcag gcgcattccc ggcgtgcaaa acagcgttgg cactacgccg ataatgcgat
    41401 ccggcttcca gcgacgttgc gccatcagtg gaaagaaact gctgacggca aaactgccga
    41461 gatgcaacaa gcgtttcagg gtgctcggct gttttggcac gtacagcggg cagcgccaca
    41521 ccgtggcggc cccctcttct cgtttgtagc gccaggcgga atagttctca cccacctgcc
    41581 attgcgggta gtaaggcggt gcggtaatga cccgcacctc atgaccttgt gccgccagcc
    41641 attccaccat ctcgccggtg tatttgccga tgccggttaa ctccggcgag tagttaatgc
    41701 cgtagaccag tattttcata atccgggtac tccggcacgc ttctcagcga gaaaataggc
    41761 gcggctgtta gcgtgaacat cgttactggc gagcagcgcg tccggcgtca gccagcggta
    41821 atcgtcatgc tgctcatccg gcattaacag ctcttcttcc gctactctga agcgaaaacc
    41881 gagcaccaca tagtgagtgg tgaaatccgt gccggagaag ttatcgtcat aaaagtgctg
    41941 ccagacaccg taaaactggc ctgctgttat cggcagacgc agccccagtt ccgccatcgt
    42001 cagccgctca aatgcggctt ccagcgtttc gtctttctgc acgcgccctc ccggcacaaa
    42061 ccagtaaccc tgcgccgggc ggttggttct tttgccaagc agaaactcgc cgcgactgtt
    42121 ctcgacaata aagtcgagag agacaagcgg tgtggagcgc actacagtgg caaagtcttc
    42181 ctgacgtaaa aacatcattc cccccgaaag cggtcttgat tctcaaggaa ccactggtaa
    42241 gtgctggcaa gccccgcttc cagtgagatt tcgtgatacc agccaagctg atgcaggcgc
    42301 gtcacatcca gcagtttgcg cggcgtacca tccggtttgc tggcatcaaa aaccacccga
    42361 cctttgtaac ccaccacttt ggcgatggtt tgcgccagct cgcggatagt gcagtcaacg
    42421 cccgtaccaa cgttaatgtg cgacagcatc ggctgggtgt tctccagcca gacttcatgc
    42481 gccagctcca tgacatgaat gctcgccgcc gccatgtcat cgacgtgcag gaattcacgc
    42541 atcggtgtac cgctgcccca taccaccacg tccggcgcat tctgtgccgt cgcctcgtgg
    42601 aagcgacgca gcaatgctgg gatcacatgc gagttactcg ggtggaagtt gtcatgcggg
    42661 ccatacaggt tggtcggcat gactgagcgg taatcgcgtc cgtactggcg gttgtatgat
    42721 tcgcacagtt tgatcccggc gattttggca atggcgtaag gctcgttggt cggctccagc
    42781 gtgccctgca ataactcgct ttctgccatc ggctgttttg ccagtttcgg gtagatacag
    42841 gacgatccaa gaaacagcag tttgttcacg tcgttctgat gcgcggcgtg aatgatgttg
    42901 ctctcaatca tcatgttctg gtagatgaaa tccgccggat aggtgttgtt ggcaacaatg
    42961 ccgcccactt tcgccgccgc cagatagacc tggtcaatgc gttcgctggc aaagaaatca
    43021 tgcaccgcgc ggctgtccag caggttcagc tcgtcgcggg tgcgtaatac cagttccaca
    43081 tcaccgcgct gttcgagctg ccgcctgatg gcagaaccga ccattccgcg atggccagcg
    43141 ataaaaatgc gttgtctact catgcttatg actccagcgc gatcgccacg tcgtagccgt
    43201 gagatttcag cagagagtgt tttttcgccg cttcgaggtc attagccacc atttcagaca
    43261 ccatctctct gagggtgatt tccggtttcc agcccagttt ttcgtgcgct ttggtcgggt
    43321 cgccgagcag cgtttcaact tcagccggac ggaagtaacg cggatcaacg gcgataatca
    43381 catcacccgg tttaacgccc ggcgcgtcat gcccggtgac ggaaaccaca atgcccttct
    43441 cttcaacgcc cgtgccttca aagcgcagtt tgatgcccag ctgtgctgcc gccatttcca
    43501 cgaactgacg cacggagtac tgaacgccgg tcgcgataac gaaatcttcc ggctgttcct
    43561 gttgcagcat catccactgc atttttacgt agtctttggc atggccccag tcacgcaggg
    43621 aatccatatt gccgaggtac aggcacgact ccagcccctg ggcgatattg gcgattgcgc
    43681 gggtgatttt gcgggtaacg aaggtttcgc cgcggcgcgg ggattcatgg ttgaagagaa
    43741 taccgttaca ggcgtacatg ccgtaggatt cgcggtagtt aacggtgatc cagtaggcgt
    43801 acagtttggc gacagcatac ggagagcgtg ggtagaacgg cgtggtttct ttctgcggaa
    43861 tttcctgcac cagaccatac agttcagagg tggaagcctg atagaaacgg gttttctttt
    43921 ccagaccgag gaagcggatc gcctcgagca ggcgcagcgt acccatcgca tcgacgtctg
    43981 cggtatattc cggtgactca aaagaaaccg caacgtggct cattgcgccc aggttgtaaa
    44041 cttcatccgg ctgcacttca cgcaaaatgc gtgtcaggtt ggaggtatca ctcaggtcgc
    44101 cataatgcag atggaatttc gggttgcagg tgtgcggatc ctgataaatg tgatccacgc
    44161 gctcggtgtt gaatgacgat gcacgacgct taataccatg cacctcgtaa cctttttcca
    44221 gcagaaactc tgccaggtaa gaaccgtctt gtccggttac accggtgatg agagcgactt
    44281 ttgacatgtc ttattcctct gtattttttg aatttattca gtttcaacgc gttcgcgtat
    44341 caccactgcg ggattccccc ggcaaaccac atttgccgga agcgttttaa aaacactgct
    44401 tcgtgcaccc acgacggtgc cgtcgccaat cgtgacgcca ggggcaacaa aaacatcggt
    44461 tgccagccag catttctcgc caatcacaat aggcgtagca ttaatggtga aatgttgact
    44521 tgcatggtcg tggctaccgg tgcataaata acttttttgc gatatcaccg aatgtgcgcc
    44581 aatggttatt tcaccgaggg tatataaatt gacgtcatcg ccgacccacg cgtaatcacc
    44641 taaggttaat ttccacggat aggtaatttt tactgacgga cgaataacta cgttttttcc
    44701 tatttttgcg ccgaataaac gtaataaaaa tgcccgccag cgatacaata cttgtggcga
    44761 ccaggcaaat attgttgcct gtactgccca ccataattgc actttaatag cgttgccgcc
    44821 ccggaaccct ttcggcaccg agaaaccgct taaatcttgc attggttctc cttatattca
    44881 gactttgtta tataaggctt tcgtctttga ggtagtacgt tggcgtaaat gccaggataa
    44941 ttcagcccaa aagccaggta catgtaatat ttgtcgttgg acttttttcg cgtcagcaca
    45001 caattccata ttattggtgg tagatacccc acccatggaa aattcagaca ccaggccatt
    45061 gagtttttta aatgcataac cggctttata cattttggct gccagcgcgt agtcggaaga
    45121 aactttatat tccaggtcat aacgccattt tttcaagccg gatacaggga aaaatatcgc
    45181 ctgatgactg gcgggcaggc tgtgataaat ataccagccc ggtttggcgc tacgtttaat
    45241 tttatgcccg tcgccaaaat ccagcagcgc atcgccggtg atcatcatgt tatctttttg
    45301 catttttaac ttacggacaa aatttgcggc atcctgatga aaaatatcgc ccgaattgag
    45361 aaacaacgcg aacttgcctt gtgccatcgt aatgcctttg ttcatggcgt cgtagatacc
    45421 gttatctggc tcgctgacaa agcgtaggtt atagatacca ttgagatttt ccagatactc
    45481 acgggtgccg tcattggaac cgccatcgac aacaatccat tcgaagctga tatcttccgc
    45541 ctgcgccaga tgcgccagcg aggcatgtgt tttgactatc ccttcgaggt tacgaaacgc
    45601 gacagtgatt atgctaagca acatgttctt attcttacct cgtaatattt aacgcttttc
    45661 gcaaaataaa cggacagacg attaaaaatg catattccgg gctaaatatc gaaccggtaa
    45721 aaaacagcga taccggagta aaaagatata attgcacgcg aaaattgcgg ttatcaccaa
    45781 aagcgttgat catcattttt atcactttcc ccatgtacca cagcgataaa aacactgcga
    45841 accaggaaaa ataaataatc agcagataca aaccattgtc tatggttttt ccgacatccg
    45901 caccgttaaa tattccgaat gatgcgacgt attcataaag tgagccaaat ctgactacac
    45961 cgtcaatatg ggtcaaagaa taaccgacca tcaccagcgg accgacaata cgataatacg
    46021 atgacgatcc ttccgtacct aaatcgccca gacgggtgga aatataagga aacgcgatta
    46081 ccacaccaac caggaataca gccagagaaa tcaatgccaa cggtaacttt ttcttaatcg
    46141 cctctttatt cagatattga aatgcccact ccagcaaata aaacaggata aaggtcataa
    46201 cccctgaaaa cgatccggat aatattatcc ctgcgagaat catagcatcg gttttaggcg
    46261 ttttgatacc aaactgtttg atgctgagcc aaattgagat taatgccaga gcgaaaaatg
    46321 ccggttcgaa ataaagtgct gtcgtgcgct tgccgccgaa tttaatgaaa ttcagtacat
    46381 agctgttgct gtaaatcaga tatttcgaaa tcgactccat aatactgctg ccaccggtga
    46441 ggataatttg cgccatctcc accaccgcca gcgccaccac cagcccgacc accagataaa
    46501 agaaacgtaa tatcttgcga tggttgtgtg gcgaaatcgt tttaaagcga atactccaca
    46561 ccatgccaat aatgatcaca atatagacaa acagcatggt tgatgtgacg tatttactgg
    46621 catccaacga ctgaccaaac agatagttga acgccgtgag tcccgcacca atccctaagg
    46681 caatcatcaa ttttttaacg ctgatgcgtt ctaaaaacag cagtaacaag acaggtaaaa
    46741 aagtgacgat agtgatgggg aaactttcac caagctgggc gattttgacg ttaaccagca
    46801 ggtagatcag cggcagcagc aggtagctac agattctgat agaagttgac atactcctcc
    46861 agcatctgtt gtccactgta ggcggcgcgg ctgcgttggc tgaactcagc cagcgtggta
    46921 ccaaatatcg cctgcgcgat ttccggtttg cttaactgaa ccagttgcag cacgtcttct
    46981 tcgctgacag tttttcctcc ggatttttgc agcacttccc gcgccgcatc gctatgggtg
    47041 gcgatcaccg gcacaccaat cgatagcgcc tcacacaaaa tcagcgggta gttatcgaca
    47101 cgagaactga acaccagcgc atccatctga ttgagcgcgc tcatcaactt gcgcttgtca
    47161 gtttcaaagc cgtgattaac cacgttgcca gcggtgaacg gcgagaactt accaaaggta
    47221 tgcagttcga ttttgtcgcc cagcgccatc atctcacgca ccagttgctg gttagttttg
    47281 ccgtcgtaac gcaggtcatg cgccaccacc gcgattttcg gcttgccctg ggtttcgcgc
    47341 accggaggca gatccgccag aatcgcttcg gttgccatat caatgccatt attgataatc
    47401 cggcaacgcc ctggaccgta caggctattg aaagcatcag ccacatgctg gctgggggaa
    47461 ataaactgac agcccagcgc cagcatctca cgaaataact ggcgtttgcc cgccaccagc
    47521 tggtgcgcgc gatcaatctt caccggcgga tagttattta aggtcgggca tttctggcaa
    47581 cccgttttcc agccttcgca accgtcggta aaggcgcagc gcccggttac actccagtga
    47641 tcgtgcagcg tccagaccac agtgacgtcc ggtttatggt ttttcacttt ttcgcaaaag
    47701 cgcaccacgc tcttaagatt tagccagtag ctgtgcagca catgaaaatg caggaccacc
    47761 ggacccggtg tgcgagtaat ggtgcgatat aactcattga aattgccaaa cagatcgcga
    47821 ttaaacagac gaaacagtgc aatgttcgcc atcgcggtca tccgcggcgt atgtttgatt
    47881 acctgcggat agttttgatg gctgacgctc tctttgccgc ctttgccgta gccgtagaca
    47941 aaatgtgacg ccagcccctg ttgcagcgca cgctggtgga gatctaacgc cacacctgcc
    48001 gctccgcctt ccgccagtcg cacattaaat tgcagaatat tcatttaatt accttcactc
    48061 gcgctttttc tcccaccacc agcgcgttgt ccgggacaga gtcgagcacc acgctgcccg
    48121 cgcccacggt gacgttgtta ccaagcgtga tatcaccaag aataatgacg ttggcaccga
    48181 gttcgacgcc gttgccaatg tgtggacatg ccatgttatc ggcaccacga ttgccgatag
    48241 tgacgccgtg gcgaatggta aaatcatccc ccgcaaccac gtttttattg atcactacgg
    48301 cgtaaccgtg atggatagta aagcggcggc caatggttgc ggctgcctgg atttcataac
    48361 cgaaaaagca ttcggtgata atgcgataca gcaccagcag cggtgccgcc cacagattgt
    48421 tgaggacgtt ctttttgcgc cacaccgaac aaaaatgagc aacacgatag gcaagaacca
    48481 tgcagcacgg gcgtaaactc cagctgttgg cgcgcagatc ttccagcatc tttagcgccc
    48541 ccgaatacca tcagccagac gtttgccgtt acgcaccgac agcagcgtca acaaggtgcg
    48601 ccaggtcatg cgtttattac ggatctggta gagggtaaac agctgatatt ttttgcttgc
    48661 acggtcaaat ttatctttgt gctttcggta aaagtggaag tacccggaga attttttcgg
    48721 tgacgaggtg atctgcattt caccgtgatt gatatgcagg atctgcgtcg cttcttccac
    48781 tttccacggt tcgccgtact ccaccaccat ccgcaggaag atgtcgtaat cctgtgctgc
    48841 tttcagttcg gtatcgaaca aacactcttt gaaacgccat gcccaagtaa agacctgatt
    48901 gccaatgata ttgcgtttat agaacaggcg gcgtgaatac ggcgatttgg gatatagcgg
    48961 caaacttgct ggttgggaat agacttcgcc ctggcaaacg tagtcgttag cgtacaaaaa
    49021 ggcgtgagtg accagttgct gtttatgggc gaggaagacg ctcagacggt tgggtgtcca
    49081 ttcgtcatcg tcatcaatcc cggtgatgta ttccccttgc gccagcataa tcgcctggtt
    49141 acgtaccgcg cacgccccgc tgttaatgtc gttgtgaatg taggtgatac gcggatcgtt
    49201 gagggcagtg acgtactgtt gcagttgttc ccaggaggtg gagcaatcat ccacgatgat
    49261 catctcccag ttgctgtagt cctggcgcag gaccgatttt atcgcccgaa tcgccagttg
    49321 ttggcggttc caggtcggca tatagattga aatcagcgga ttgtctttca taggttgttc
    49381 tcccggagtg tcgagatgtt ctgccggttt ataagcttgt ttacgggttc tgttgccgga
    49441 tgcggcgtga gcgccttatc cggcctacgg agctggtttg cttgccggat gcggcgtgaa
    49501 ggtaggtcgg ataagatgcg tcagcatcgc atccgacagc ggatgtcgtt atttcgcatc
    49561 cgacttatat tcgtattcgt aatagccata atcctgatac gcgctggcgc ggcggaagat
    49621 ggagttcaga atcacccctt tcaccggaat accattttgc tcaaagcggc tcagactggt
    49681 ttccacttct ttcaatgtgt tgaccgcata acgcgccacc attaacgtgg ttccaacatg
    49741 acgaccaaca attgccgcat cggtcactgc cagaatcggc ggcgtatcaa tcaacaccag
    49801 gtcatagttt ttactcgccc agttcaccag ttcggcaaag cgttcgctca tcaacagttc
    49861 agaaggattt ggcggcacct gaccgcgcgg gatcaggtca aatttggcaa tagaggtcgg
    49921 tttagcagcg gtagtaatat cgccctgacc aatcagaatt tccgacaggc cattaacgtt
    49981 attagtgccc agcagctcgt gggtgtagcc tttgcgcata tcgcagtcga tcaacaacac
    50041 ccgtttattg gtctggctga tcaccgccgc caggttggcg cagacaaagg ttttaccgat
    50101 tgacgggcta accccggtca tcatcaacac attgttctgt gcctgcatca tcgcgaagtg
    50161 caaactggta cgcagactgc ggatggcttc aatcgccaga tcggttggat tccccatcgc
    50221 cagtagctgg ctctgtttat agcgtttaat ccctttgatg gttttgacgc tatcgcgcgc
    50281 tttctgccat tccgaaagcg ggatgctggc atagacgctg ataccgtgtt cttccagcac
    50341 ctgcgggctt tcgatgccgc gattaaacaa cgagcgcagc agcacaccca cgatagaaag
    50401 catcaggccg aggataatcg ccccaaggat aatcagctct ttcttcggtt tcagtacacc
    50461 tggctgggtg attgccgggt caacaatgcg cacatcgccg acggtgctgg cctcggtgat
    50521 tttcagctcc tgctctttat tcagcagttg catatagacc tgctgaccag actcgacatc
    50581 gcgggtcaga cggacaatct cctgctgggt tttcggcatc gccgttacgc gaccgttaag
    50641 tttggctttt tcgtcttcca gcgcctgacg tttctccagc agcgtgcggt acgccgggtg
    50701 aactttggtg tacagcttgg agatttccgc ctctttaaag gtcagttcgt tcaactgggc
    50761 gtcgatgttc accatcgaat cgagcaccgc tttcgcttcc agcggcagat caacagaatc
    50821 tttatcctga cggaaggcat tcagtttgtt ttcggcaaca tcaaggcggc tacgtacttc
    50881 cggtaactgt tgcgcgagga aagcgaggct tttcgacgct tccgccgatt tgcgctcaat
    50941 attttgttcc tgatagttac gggcgatgct gttaagaatg tcgcggatct gttcgcgatc
    51001 ttcaccggta taagtcaggc tcagtacgcc tgcgtctttg ccgttctccg ttaccgtcag
    51061 gctgttttgc agttgattga tcatccccag cgtggagtat ttggtgacgg taaactcact
    51121 gcccgggcgg gcgtgaatgg cttcaaccat cagcgtgacg ccttcttttt tcagcatctg
    51181 gcccgcttgt ccacgggcgc taaagccgcc atcactgctc agggtgtagt ttttatcgtc
    51241 cagcacatta agcgtaaaca cctgatccgc catctctttc gggcggttaa aggtggtcac
    51301 tttcaccgtc tcgttctgac gtcccatcaa acgatcccag cccgcaccga aaatcgggaa
    51361 cgtgtttttg ctcaccgcaa tatcgaggtc gagatcgtcc accgttttac caagcaccag
    51421 acgcgaacga atcaactgga tctcggcgtc cgatgcaggc ggtttgttgg ctaatgccga
    51481 accaatgtcc tgcactaacg aattgccgct gttttgctcg atttgtacca gtgcgtcggc
    51541 actataaatc ggcgtggcga agaaggtgta aaccacggca cagagggcaa atacagcggt
    51601 gatgccaatc acccaccagc gcgcttcaat gacggtgccg accaggcgac caatatcgat
    51661 ttcatcactg cccgttaccg gagcggcatg ttgttttact ttttctgtca ttcttatacc
    51721 tgctctgcgt tcaatgcctg cgcccactgg cgggcagacc gttcaagtaa tgtgtacacc
    51781 gctgcaaacg tttcccggct tttgcgatac ggatcgggga tttcacattc gttatcccag
    51841 tgaccaaaca gcatcacttt gccgcgcatc tcaggtgcca tctcgcataa gcgttcgata
    51901 tggcgctttt ccatggtcaa aatcaggtcg tagttgcgac acagacggcg gctgatttgc
    51961 cgtgcacagt gaccttccag agacagttga tgttctgcgg cgatgctgat agcagtaggg
    52021 tcagcgccct taccgaccag cgcgccgagt ccagcggact ccactttcag ctccgggtga
    52081 taacgttgca gtaagcgttc cgccgtcggg gagcggcaaa tattgccgac acagacaact
    52141 aagatgttgt taaacatgac gattaccagt tatgaatgtc gctggctgta tccgtcatgt
    52201 aacggacacc gctaatagtt ggcagcaact gattgatcag acggttccag cgggaaaccg
    52261 gggcggtggt gacatacacc acgtcataag gttgcaggcg gaattctgtc gccatcacca
    52321 gcgacgtggc atcggacata tccagctggt agatattggc aatcttgccg ttacgcccgc
    52381 cctcgccttt cagcggacga atgacaaaga tgccgctggc gttggaggtg gtcatgtcga
    52441 taccttcagc attacccagg gcttcagtca gggtcatgcc gctaaagtcc attttcaggg
    52501 tgctctgttt cttcacttca cccatcacaa atactttcag atcatcatta cgcggcacgt
    52561 agagaatatc gccgggataa agcagtcggt tctgattgag gtcgccgttt tgcatcagca
    52621 cctgcaaaga aatgcgttct tcacgaccat tgtgtgttag caccacgttg cgccagtcag
    52681 cggtgtcggt caggccacct gcggcgttga tggcgtcgag aatggtcagt ggtacgttgg
    52741 tgatcgcctg ttgaccggat ttattcacct gacctgagat ataggtcttt tgtgagcgga
    52801 aggcggcgat attaacgtcc acctgcgggt cagcgatgta cgtcgctaag cgcccggtaa
    52861 tatcactgcg gatttcagcg agcgttttcc cgactacgtg aaccttgccg atatacgggt
    52921 aaaacatagt gccgtcaggc tgtacccagt tgccggtgtc gctggagctg cggtactgac
    52981 cagctggcgt ggtgagttcc gggtgatccc agacggtgac attaagaacg tcccccggcc
    53041 cgacgcgata ctggtaattc gcgatctcgc tttccagcgt catattgggg cgcgctacat
    53101 tcgggcgtgg gcgtaattgg tcaatcaggc gcggggtcag cggataaaca ttcaccattt
    53161 tgtcgagatc gaaatcagcg tcctgctgtt tgatgacgtc tttgcccatc gtcgacatat
    53221 tgctgcccgg aagtactgtg caaccgctta tcaaggttac tgacaccaat aatggcatca
    53281 atttcatttt ggatttcatc attgtttatt tatcactttg gcagagtaat tatcctgtgc
    53341 actattaata gcaatgtcgc catgcacatt taccttgcag ttaattgaat acaaatttaa
    53401 ctggcatcag tcctaaaaaa attgatttca tccgcaggct attgacagaa taattcagac
    53461 tggtctttca ggcatccaga cacgctaccg cccctggctt tttagccacc aatacactga
    53521 tttagcttaa ttttttaaac cctctctgca tacaatcgtt gatgagaaag ggttattgcg
    53581 gaaattaact tccgaatata aggtgacatt atggtaattg aatattggct ctccaataat
    53641 gcaggaggaa gtgttacagc taacggaata gcaggcaagg taacaattcg gcaattggct
    53701 atttttaaga attatattaa gtgtcattca atatggtttt taggagtttc tttaggttga
    53761 caatatttaa tattgcgcct ccgcatgcga tatttcttaa ataatgtttt attattacca
    53821 cttttaattc agggataatg tgaggttatt accctcaaat aatatgagat aaatagtgct
    53881 gcaacattgc atttttgccc cgatttatcc atgatcgaat tgtgacattt gtcatacaac
    53941 gaataggttt tgtacttact atggaatgga ttgccgatcc gtctatctgg gccgggttaa
    54001 tcacgctgat tgtgatcgaa ctggtcctcg gcattgataa cctggtcttt attgccatcc
    54061 tcgccgaaaa actaccgccg aagcagcgcg accgcgcacg ggttaccggg ctgttgctgg
    54121 cgatgttaat gcgcctgtta ctgctggcgt caatctcctg gctggtcacc ctgacgcaac
    54181 cgctgttcag cttccgctcg tttaccttta gcgcccgcga cttaatcatg ctgtttggtg
    54241 gtttcttcct gttgttcaaa gccacgatgg agctgaacga acggctggaa gggaaagaca
    54301 gcaataatcc cacacaacgc aaaggtgcga agttctgggg ggtggtgaca caaattgtgg
    54361 tactggacgc catcttctca ctcgactcag tgattaccgc cgtcgggatg gtcgaccatt
    54421 tactggtcat gatggccgcc gtggttatcg ctattagcct gatgttgatg gccagcaagc
    54481 cgttaacgca atttgttaac agtcacccga ccatcgttat tctctgctta agcttcctgt
    54541 tgatgattgg ctttagcctg gtggcagaag gtttcggctt cgtcattccg aaaggctacc
    54601 tgtacgctgc cattggtttc tcggtaatga tcgaggcgct caatcagttg gctatcttta
    54661 accggcgacg ttttctttcc gctaaccaga cgctacgcca gcggactacc gaagcggtaa
    54721 tgcgcctgct tagcgggcaa aaagaagatg cggaactgga cgccgaaacc gcgtccatgc
    54781 tggtggatca tggtaaccag cagatcttta atccgcagga acggcggatg attgagcggg
    54841 tacttaatct taaccagcgt actgtcagca gcattatgac gtcgcgccac gatattgagc
    54901 atatcgatct caacgcgccg gaagacgaga tccgccagtt gctggagcga aatcagcata
    54961 cgcggctggt ggttaccgac ggtgatgacg cagaagattt gctcggtgtt gttcacgtta
    55021 tcgacctgct acaacagtca ctgcgtggcg aaccgctcaa cctgcgggtg ttgattcgcc
    55081 agccgctagt gttcccggaa accttgccgt tgttacctgc cctggagcag ttccgtaatg
    55141 cccgcacaca ctttgctttt gtggtggatg agtttggctc ggtggaaggg attgtgacat
    55201 taagtgacgt cactgaaacc attgccggta acttaccgaa cgaagtggaa gagatcgacg
    55261 cccgccatga tattcagaag aatgcggacg gttcctggac ggcgaatggt catatgccgc
    55321 tggaagatct ggtgcaatat gtgccgctgc cgctggatga aaaacgtgaa tatcacacca
    55381 ttgccgggct gttgatggaa tatttacagc gtattccaaa acctggcgaa gaagttcagg
    55441 tgggggatta tttgcttaaa acgttgcagg tggaaagcca tcgcgtgcag aaggtgcaga
    55501 ttattccgct gcataaggat ggcgagttgg agtacgaggt gtgatggtat cggtcatttt
    55561 acccaatagc cgaaaggaat aagatgaggg aaccgcgcca gcggcgattt accgccggga
    55621 gcccgggttg ccagggtggt ggcgattgag cccccctggc acgttcaccg gcttgagcgt
    55681 tccatagtag cgaggaacat caagtgaacg gaacagcctt cgatcttaca tattcagaat
    55741 ataatgatgc cgccacgaac gcctgatgcg acgctttacg cgtcttatca ggcctacagg
    55801 acaggtcacc aggtaaccta catcttctcc agcaacttct tcacatcttt gccattgcgg
    55861 gaatctttat tccgctcggc ccagtcattc agacgacgtt tcgcttcgtc ctgtagatgt
    55921 ttgcgcagta actgatccac ttgcagactg taattgagtt gctgccagtt gccataaacc
    55981 cgcagcggta ctggcgtttc tttaaggaaa tcaatcagtt tgctttcccc gttccagcca
    56041 cccacgaccc gaatatcaaa ctgggtgtcg caggtttgct ccgccagatt caacgtacca
    56101 gccccggaaa gcgccagcac aggcgattga ccttgcatgt cgtttaacgt cacgacgcca
    56161 tccttcaacg tcaaatcggt ggtaaagcgg tcaagacgcg ttacgttatc gaagttttca
    56221 gcggccttca catcaccacc attacgttct accgcttgct gaatcatctg ctggaagttc
    56281 atcccttcca tgcgcgtgtc ggtcatttcg acatgtgcct gtccttgcca gttgtggcgg
    56341 aatgcgtcgg catctatgtc agcaccggag aaatcaccag ccagtgacat ctttccggtc
    56401 aacgaaatcg gatagttaaa cgccttcaga atggtgccaa tctcaacgtt ttccagccgt
    56461 ggctggaagt ttatccgcgg atttattgat gtcgcgtcca gcgtgcccgg cagtgaaacc
    56521 tgtccaccgt taagcttgcc ctgcagttga gtaatttcca gcaaacccga cttgttggtc
    56581 atttgcgtgg caacatctgt aaaattcatt ccgcgccagc gcacgttact ggcctgcaac
    56641 aaaatatcag ccgtaaagcc ttgcagtccc tgatatgccg gttcatcaat acgcgaagaa
    56701 atgaccgggc gcggcaacgt tgattggctc tgcccctgct gcgcggcacc gttttcacca
    56761 ttcgctgttt cattaagcgg gatgaggttg tcaagattca gttgcgggaa ttgcagtctc
    56821 agctgccatt ccggtttctc tgtcagcgtg acctgtgctt gcccgctcag cgtactgtca
    56881 ttggcggtca aactaatttg gttaaatgaa aggcgtttat gtgactcctg ccactgggcc
    56941 tggaaactcc cctgcccatg aataccttgt tttggtaaat ctgcaccctg caactgccag
    57001 ttaatttgtt caatagccgc cgttaaatca tgcggataat cagaagcatc taccgtaccg
    57061 ttaagggata tcgtgagatc gcgctgatcg cgattaaccc gcccggagaa ctcaaatgaa
    57121 ccacgatgtt ggggatcttg ttccatttgc aggcgaatat tgcggattgt cacctgctcg
    57181 tcatcttcat gctggaacac cagcacgctg tccgccacct taagactgga tatatcaaac
    57241 gaccatccgc gatcgtctga cagatccggc aaggtattgt cgcgcggtgc aaccggagcg
    57301 tcttcactgc gcaccacttc cgtctgcggc gtcagttgga tcactgcccc ttttagcatc
    57361 acctgcttaa cgctcagttg atgactcagt agtggtaaaa gcgccacgtc cagacgcatg
    57421 ttgtcggcgc gaaccagtgg ctggctcgcg ccctgggcgg tgagagacat tcgcccggag
    57481 aggatactaa gctgcggcca gacgtgccaa cgcagtggcc cgtcgagctg caattgataa
    57541 ccgctacgtg cagcaacttg cttgaccata tagttgcgga aatcattcgg attcaccagc
    57601 aacactaacg cagataaccc ggccaccagc acgaccagga gtatcatcag cgtcgtcaga
    57661 aatcgtctca tggtatcctc aatgggcctg aattagtctt tatcgattcg gctggctacc
    57721 gcgccctgct ggttgcgata tttcgcatct tcacggcggt tgtaaggtcg cgccgccggg
    57781 ccggaaagcg gctcaaagct cagcgcacca attaacatgc ccggacgcag cgccagcggc
    57841 agcttaccgg agttgtagaa ctccagcaca atgcaaccag accagcccgg atcgatacgg
    57901 tgcgcggtga cgtgcaccat cagccccaga cgcgccaaag aggaacgccc gtccagccag
    57961 cccaccagat cggctggcag cgtcaccgac tccagcgtca ccgccagcgc cagttctcct
    58021 gggtgaagat agaacgcctc gccctcgtcg agaacgatct catcgctcat cacgcggtca
    58081 agcgcggcgc tcacttcatc tttgggaccg ctcagatcga taaacgctgc cgtgtgacca
    58141 cggaaggtac gaaatttatt gcccaggcgt acatccaccg tcgcgccgtt aatacgctcc
    58201 actggcggac gtgggttgat cgacaaacgg ccttcatcaa gccaggcttc aatatctcgg
    58261 tcacacagac gcatggcatt ttctcctttc gcgcatcact cccttaacgc caattacgtc
    58321 aagggcatac caggttatca ctgaacggta cacaatttat caagcttatt caaagaactg
    58381 acttattttc gctttcaata tatcgatcgc gatgcggttt ttcccgccgc gcggcacgat
    58441 aatgtccgca tattgtttag aaggctcaat gaattgcagg aacatcgggc gcacggtttt
    58501 ttgatattgc gccatcacgg aatccattga acgcccacgc tcgttaacgt cacgcttgat
    58561 gcggcgcatc aggcagatat ccagcggggt atcaacgaaa atggagaagt taagttcgtc
    58621 acgcaaacgc gcatccgtca gcaacaaaat gccttcgaga atgatgacct tcttcggctc
    58681 aaccgtcacc gtttctttca tacgcgtatg ttcaacatag ctgtaaaccg gcaggtcaat
    58741 tgccgagccg cgtttcaacg cttgtaaatg ctcaagcagc aggttgtgat ccatcgcgct
    58801 gggatggtcg tagttggtct taacgcgttc ttccatcgac agatggcttt gatctttgta
    58861 atagcagtct tcgggaatta cgccgatgtg ttcatcaccg acttgctcac gtaattcacg
    58921 ataaagggta ctggcaataa gactcttgcc ggaagccgat gcgccagcga taccgataat
    58981 gacgcactga tgagactgat cagtcatata tttagcgacc tgattaacct ggatgttagg
    59041 aagggggcga cgaagcgcca aacgcggcaa ttatagggat ttcatccgcc tgataccagt
    59101 cgaatagcgt tgccgcgcgc tcagagttaa ttgttgacaa agaattcccg gtggcaaatt
    59161 acgttgatca gttttatgta aggtaaaaaa tgttatacgc agttgcgcaa attatccgcc
    59221 tttacgtcac tttatgagca attcacatat aaaatgtaaa acttttgtac tagcataaac
    59281 acagaaacga atactggcga cctggtcttg cggataaagc ggtaatgagc aaacaatcac
    59341 agcatgtatt aattgccctg ccccacccac tgcttcacct ggtcagttta ggtttagtct
    59401 cgtttatctt tacccttttc tcgcttgagc tttcgcagtt tggcacccaa ctcgccccac
    59461 tgtggttccc gacgtccatc atgatggtgg cgttttatcg ccatgccggg cgcatgtggc
    59521 cgggaattgc gctgagctgc tcgctgggaa atatcgccgc atccatcctg cttttttcca
    59581 ccagctcgct gaacatgacc tggacgacca tcaatattgt tgaagccgtg gtcggggcag
    59641 tgctgctacg taaattgctg ccgtggtata accccttgca aaatctggct gactggctgc
    59701 gtctggcatt cggcagcgcc attgttccgc ctctgttagg gggtgttctg gttgtcctgc
    59761 tgacgcccgg agacgatcct ctcagggcat ttttgatatg ggtactgtca gaatccatcg
    59821 gcgctctggt actggtgccg ctgggattgt tatttaagcc acactatctg ctgcgccatc
    59881 gcaacccacg gttacttttt gagtcactgc tcacattagc catcacgctg acgttaagct
    59941 ggctttctat gttgtacctg ccgtggcctt ttactttcat tattgtgctg ttgatgtgga
    60001 gcgccgtgcg cctgccacga atggaagcct ttttgatctt ccttaccacg gtgatgatgg
    60061 tgtcgctgat gatggccgcg gatccctccc tgcttgctac gccgcgtacg tacctgatga
    60121 gccatatgcc gtggctactg tttttgctga tcctgctgcc cgccaacatc atgaccatgg
    60181 tgatgtatgc ctttcgtgcg gaacgcaaac acatttccga aagcgaaacc cgctttcgga
    60241 acgcgatgga atattccgct atcggtatgg cgttagtggg caccgaggga caatggctgc
    60301 aaaccaacaa agcgctctgc cagtttctcg ggtacagtca ggaagagctg cgcggactca
    60361 cctttcagca actgacctgg ccggaggatc tcaataaaga tctccaacag gttgaaaagc
    60421 tgataagcgg tgaaataaac acctattcaa tggaaaaacg ctactacaac cgtaatggcg
    60481 atgttgtctg ggcgttgctt gccgtctcac tggtgcgcca cacggatggc acgccgctct
    60541 attttatcgc tcagattgaa gacattaacg agctaaaacg caccgaacag gtgaatcagc
    60601 aactgatgga gcgcatcacg ctggctaacg aagcgggcgg gattggcatc tgggagtggg
    60661 agctgaagcc gaatattttt cgagctgtat gaaattcctc cgcatatcaa accgaactgg
    60721 caggtgtggt acgagtgcgt gctgccggaa gatcgccagc atgccgaaaa agtgattcgt
    60781 gattcgttgc aatcacgctc gccctttaag ctggaatttc gcattaccgt aaaagacggt
    60841 attcgccata tccgcgccct cgccaaccgg gtactgaata aagaaggcga agtcgaacgc
    60901 ctgctcggca ttaatatgga tatgaccgag gttaaacagc ttaacgaggc attgtttcag
    60961 gaaaaagagc gcctgcacat tacgctggat tccatcggtg aagccgtggt ctgtattgat
    61021 atggcgatga aaattacctt tatgaatcca gtggcggaga agatgagcgg ctggacgcag
    61081 gaagaagcat taggtgttcc gctcctgacg gtgttgcata ttacttttgg cgacaacgga
    61141 ccattaatgg agaacattta cagtgccgac acctcacgtt ccgcgattga acaagatgtg
    61201 gtgttgcact gccggaacgg cggcagttac gacgtgcatt acagtattac gccgttaagt
    61261 actctggacg gcagcaatat aggctcggta ctggtgattc aggacgtcac cgaatcacgc
    61321 aaaatgctgc gccagctgag ctacagcgcc tcccatgatg cactgacgca tctcgccaat
    61381 cgcgccagtt ttgagaagca actgcgtatc ctgctgcaaa cggtaaacag tacacatcag
    61441 cgacatgccc tggtgtttat cgatcttgat cgctttaaag cggtgaatga cagcgccggg
    61501 catgcggcgg gtgacgcttt actgcgcgaa ctggcgtcgt tgatgctgag tatgctgcgc
    61561 tccagcgacg tgctggcgcg actcggtggt gatgaatttg gtctactgtt gccagactgt
    61621 aatatcgaaa gtgcgcgttt tatcgctaca cgcattatca gtgccgtgaa tgattatcac
    61681 tttatatggg aaggccgtgt gcatcgggta ggtgccagtg ccgggattac cttgattgat
    61741 gacaacaatc atcaggcggc agaagtgatg tcgcaggctg atatcgcctg ttatgcctcc
    61801 aaaaatggtg gacggggccg ggtgacggtt tacgaaccac agcaagctgc cgcacatagc
    61861 gaacgggcgg cgatgtcgct tgatgaacag tggcggatga ttaaagagaa tcagttgatg
    61921 atgatcgccc acggtgtcgc ttcgccgcgg atcccggaag cgcgtaattt gtggctgatt
    61981 tcacttaagc tctggagttg cgaaggcgag attattgatg aacaaaaatt tcgtcgtagc
    62041 ttcagcgatc cggcacttag ccatgctctt gaccgacggg tattccacga atttttccag
    62101 caggccgcaa aagcggttgc cagtaaaggc ataagcatcg ccctccccct ttccgttgcc
    62161 ggtttgagta gcgccacgct ggtgaatgat ctgcttgagc agctgggaaa tagccctcta
    62221 ccaccacggt tattacatct gattattccg gctgaagcga ttttagatca cgcagaaagc
    62281 gtgcaaaaac tgcggctggt gggatgtcgg atagtgctca gccaggtggg ccgcgatctg
    62341 caaatcttca actcgctgaa agcgaatatg gcagattacc tgctacttga tggtgagtta
    62401 tgcgccaacg tgcagggtaa tttgatggat gagatgctga ttacgattat tcaggggcac
    62461 gctcagcgac tcgggatgaa aaccatcgcc gggccagtcg ttttaccctt agtgatggat
    62521 acgctttctg gcatcggcgt cgatctgatt tatggtgatg tgattgccga tgcccaaccg
    62581 ctggatttgc tggtgaatag cagttatttc gcgattaact gagagagcgt ttgcccattg
    62641 cctgatgcga cgtatcaggc ctacaattcg tccgttccgt ctggttgcca gccttccgta
    62701 taccagatat gcaacagcgc ataagaacgc caaggcttcc agcgctcggc ataacggcgg
    62761 atttgtgccg gtgtcatccc cggaaatcgc tgtttaatca gataatcatc cggcagaaaa
    62821 acatctttcg cctgccagcc acgcaaagcg aaataattcg ccgtccagcg cccgataccc
    62881 ggaaaagttt gcagcgtttt catcgcctgc tccacatcgc ccggaattgt cattggtaag
    62941 ctgccctcca gcgccgcatt tgccagatga atcagtgcct ctgcccgttt caacggcatg
    63001 cctaacgctt ttaatgcctg cgggtcggct gctgccagcc gctgaggcgt ggggaagcag
    63061 atatactccg gaaaatcatc cagccgttcg ccataaagct gtacaacttt ggcagtcaat
    63121 tttgccgcca tcgccacgct caccagttgg cctaaaatcg cccgcacacc ctgctcaaaa
    63181 gcatcaatac agccgggtaa acgcaatccg ggccgcgccg cgcctaactt gcccaacgca
    63241 ccgttaacaa tctgcgggtt acattgcaga tcaaacaggc ggctcatttt cgccagacac
    63301 tcagcggcaa cgggttctaa acctgcactt aaatttatgt gcagagtatg gcgggctata
    63361 tccggaatag cagtcaccac gccgcgatat tcgcccaccg ccagactacg ggcataataa
    63421 tcgtcagcga ccgtttccac accactcact gcacgggcgg cgagaaatcc caacatccac
    63481 gaccagtcat acggcggctg ccagttcagg gtatacatcg catctcctta ttcatccgct
    63541 ttcagcataa acgttattca gacgctacgc tttgctttca tattccggtt gtcgcgacgg
    63601 caacatttcg ctaaagtcac gccccttctt caccggcatg gggattattt cgtgtttatt
    63661 ggttttgatt acggtacagc aaactgttca gtggcggtca tgcgtgacgg taaaccgcac
    63721 ttgctaaaaa tggaaaacga cagcacgctg ctgccttcaa tgctttgcgc gccaacgcgt
    63781 gaagcggtaa gcgaatggct gtaccgccat catgacgttc cggcagacga cgatgaaacg
    63841 caggcgttgt tacgacgggc gattcgttat aaccgcgaag aagatatcga tgttacagcg
    63901 aaaagcgtgc agttcggtct ttcctcactg gcacagtaca ttgatgatcc agaagaagtg
    63961 tggtttgtga aatcaccaaa atcgttcctc ggtgccagcg gcttaaaacc gcagcaggta
    64021 gcgctgtttg aggatctggt ctgcgcaatg atgttgcaca ttcgccagca ggcgcaggcg
    64081 cagctgccag aagcgattac tcaggcggtg attggtcgcc cgatcaactt ccaggggctg
    64141 ggcggcgatg aagcaaacgc ccaggcgcaa ggaattctgg aacgcgcggc gaagcgtgcc
    64201 ggattcaggg acgtggtatt ccagtatgag ccggtcgcgg ctgggctgga ttacgaagcc
    64261 accttgcagg aagaaaaacg ggtgctggtg gtggatatcg gtggtggtac gactgactgt
    64321 tcattgctgc tgatgggacc gcagtggcgt tcgcgtctcg atcgtgaagc cagcctgctg
    64381 ggtcacagtg gttgtcgtat tggcggtaac gatctggata tcgccctggc gtttaaaaac
    64441 ctgatgccac tgctgggtat gggtggcgaa accgaaaaag gcatcgccct gccgatcctg
    64501 ccgtggtgga atgcggttgc catcaacgac gtacctgcgc agagtgattt ctacagtagt
    64561 gccaacggtc gtctgcttaa cgatctggta cgcgatgccc gcgaaccgga aaaagtggcc
    64621 ctgttacaga aagtctggcg tcagcgttta agctatcgcc tggtacgcag cgcagaagag
    64681 agcaaaatcg ccctttcaaa cgctgcggaa acccgcgcct cactgccgtt tatcagcgat
    64741 gaactggcta cgctgattag ccagcaaggg ctggaaagcg ccctcagcca gccactggcg
    64801 cggattctgg aacaggtgca actggcgctg gataacgccc aggaaaaacc ggacgttata
    64861 tacctgaccg gcggtagcgc ccgttcgccg ctgattaaaa aggctctggc agaacagttg
    64921 ccgggcattt cgattgcagg cggcgatgac tttggctccg tcaccgccgg gctggcacgc
    64981 tgggcggaag tggtatttcg ttaaaagaaa atggctcagc agcggcttaa atctgcctgc
    65041 gcctgagcca gtttttctgc cgcctgacgt aacggttcca tcaacggcat ggtccgtgct
    65101 ggagcataaa ggcggaatcg ctgcaactct gtcgcgccga cagtcaacgc cgattccagc
    65161 cgatgtcgtt tggcggccat taccgcctgt ctgtctgctg gcgttaccgc ttcgttcggc
    65221 ctgaacacaa aacggcggtc acagctcgct ttccagtcga taaccgcctg ggtcagatgg
    65281 tcgccaaacc ctttaacttg cttaacgcca cgacgggtaa catccgctgc tgtttcaata
    65341 ccaaaagacc gtaacgccgc tttacgcgca gggccaacgc cgggaataga ggcaaaaaat
    65401 ccctccagaa acttctgctt ctgccgttcc cttgcggtgt cataaagtgc tgccagagcg
    65461 cgtttctcct cttccggtaa cccgagaatt tcgtccttca ttttttcgag catcgcccgt
    65521 ttggcgataa aaccttccag cccgcccaac tgttggatct ggctgactaa atgctcatag
    65581 tccattttcg cgcgattaaa tgcctcacgg cgttgctgga tttctgcttt gtacgctttg
    65641 cttgtcagac tgccaataat ccagatagcc gacagcacag gaaccaaaat aatataacgc
    65701 ggttctgcct gaaggccgca aagcaacagc gataacgctg acagtgcgat ctcaatcaga
    65761 atgatgtatt cgcgccgtaa caggcctaaa ggaagcggcc tgccagtcgg ttggaaatga
    65821 tcgggtaatg gcaattgcaa tgctggcggt gctactgacg ccatcaccat cgcccagact
    65881 ttcgccagca caaaatcacc gctggtggta atgacctctt caccgagatc aacaaaatag
    65941 ataacgcctt gattatccag cgtacaccac gggcagtcgg tcaaatgagc gggataaaca
    66001 tgcattgccg aaacgggaca tttctttaac tgttggcgca gagaatcaag tgccgctacc
    66061 cacgctttag ccgtcggacg cccggttgcc acgccacttt ccgtgaatgc ctgctgaaac
    66121 atggcttcaa catcactcgg taacatcgat agcggaatcg atcgtggcgg cggttttaaa
    66181 ccacgtcgct gattatctga cgcgtaggca taacggaaat gggtaatatc cgtctccagc
    66241 gcattacccg catcagagat aagcggcaca ccagaataag gatgccggcc accaaacaag
    66301 acgtgaaaaa tcagcaacgc aaggccaaaa ttatcgtgat tttctgtgcg ttcaaagccg
    66361 acaaatgacg acaaggtttg tagctctggc ggcgtaaaat gcgacacgcc gacttcgcat
    66421 aaatgcagtg tgccattgac gttaatctga aaggagtcgc tatcaatcag taccactttg
    66481 ctgtcgcgac ctaccataaa actgttctgg tttacgtcac ccacgacgtg cccgtgctcg
    66541 tgaaccgtag caaaagatga agcaatattg cgcgcaacat agagtagaaa atcccacgca
    66601 caatgagggt aactctggcg acgatgtgcc gggctataga tcatatgaat cggttcttta
    66661 ccagaaactt ttggcatcat aaaaccgata acttttccgc ctcgtccacc gtgaagcgtt
    66721 gcctgcggcc aggcgacata attcaacaac tgcgcgtcag ctgtcgcagc cataaaggca
    66781 agtttgtcct gttttgaggc gggtggcggc gtgtgataaa tcttggcgac gctatcgaca
    66841 aactcctcga tatcataaac cgcgccttca ccgcctttgc ccagttcacg gcctggcgta
    66901 acgcactcac cagtagcagt atataaagtg ggtttcatgg taaccacagc gccagtgcca
    66961 acgttttatc atcatccgta cgctcgttta ccgccgggct ggacaaaaac tgctttaaca
    67021 attcaggtaa taaatcgagt tgttcctgcg ttactgacgc cagtccattg aaaaacgggg
    67081 taaagaaagg cacatgagga gaattatcca gcatatttaa cgccagccgc tgaataccat
    67141 ccgtaaatgc tgcaacttta tgcgcacgcc cagtgctggt gaaagtttcc agtctggaaa
    67201 cggcatcttc gtcggtaatg aagtgcgtca tgttggcata ttcacccacc atcggcgtga
    67261 gaggcaattg cagaccgtga cccaaatcta ccaccacgcc gccatcgcca atctgcataa
    67321 tcaaggtgcc gtcaggcgat gaaatcaatc ccagaaacgt gcaggcaaaa tcgcgaactg
    67381 ctaatgcttt ggcttctgcc tcggcaaaaa gtcgttgacg aatagtcagt accatatttg
    67441 tggcgaggac atcattaagc cccaattcac cgtcctgtac tttttgcacc ataaacgcca
    67501 tcgcttcatt gacggcgagc gtcgcgcctt ctcctccctg cgaaacgcta cctgcgccat
    67561 cagcaaggaa catcaccagt aatggttgct ggtcgtttaa ccagacaacc tgcatctggc
    67621 aggcatcctg acaaggcaaa tcagcactaa tgtgcgatgt tccaacagcg gatgcataaa
    67681 ccagacgcca gctcacactg acgtccagcc ttttggcgct tccagcacaa cttccgttcc
    67741 cggcgtggag cgggaaaccg aacgcagcga actggataac cagctaaaca gttcacggaa
    67801 ttgtaatccc tgcagtggca aaggctgacg aacgctgatt tgtgccagag tcttcatatc
    67861 cgccccctga acaccgatgg aaaagaaggc aaacttctta tcttcttccc cctgaaacac
    67921 tttgttggcg gcagcctgcc actcatcggt tggagcgcca tcggtgatca ggaaaatcca
    67981 cgggcgataa tacgaaatac catttgcgcg gtactcacgt ttcctctctt caaccatatc
    68041 tagggctttg gtaatggcgg cccccatagg cgtatccccc tgggcaaaca ggatgggcgg
    68101 gaaaaagtta gcggcgctgg taaagggttg ttccacatgc accgggccga acgtcacaat
    68161 gccgagttcc actcttttta aagccagcgg atcagcaagc agttcatcac gaaaggtaac
    68221 caatccggcg ttaagttcat taataggtct gccgctcatc gagccagaaa catccagcag
    68281 caaaatgcag gggcaacgtg gttccgggtt actggcaaaa tcgctggtgg caaacgtgat
    68341 ttgttcgctc ataagaaatc ctttcttaga gaattatcga ggtccggtat cataccgatt
    68401 gcaataatga aataagagca aacgaagaaa ctttcagaat taggcccgtc ggcgcagaaa
    68461 attttgctat tcgcctcgca agaacggaga gccgtcgatt gacactaagg gcggagtgac
    68521 ataatttcag gagtgagggt tagggagagg tttccccctc cccctggtgt tcttagtaag
    68581 cctggaagct aatcactaag agtatcacca gtatgatgac gtgcttcatc ataacccttt
    68641 ccttattaaa agccctcttc tccgggagag gctttcccgt ttcagcgtcc cgctgaaatc
    68701 atcggcttac ctcctttcgc cctgaatgca gtctatcgct aacgcgtgaa taaaatagtt
    68761 tcctctgtga ttattgcgta acgactcgca aagaatattt gctggcagga tcgcagacta
    68821 caaagcctac ggattgacaa tcttatcgtg aaggcatact ttcaggagtg agggtagagc
    68881 ggggtttccc ccgccctggt agtcttagta agcggggaag cttatgacta agagcaccac
    68941 gatgatgagt agcttcatca tgaccctttc cttatttaag gccccttcct cgggaggggc
    69001 tttcccgttt cagcgtcccg ctgaaatcgt cggcttacct cctttcgccc tgtatgcagt
    69061 ctatcgctga cttgcggata aaatagtttc cagtgtgatt attgcgtaac gtctcgcaaa
    69121 gaatatttgc tggcaggatc gcagactgca aagcctgcag attgacaatc ttatcgtgaa
    69181 ggcatacttt caggagtgag ggtagagcgg ggtttccccc gccctggtag tcttagtaag
    69241 ctggccagct aatgactaag agcaccacga tgatgagtag cttcatcatg accctttcct
    69301 tatttatggc cccttcctcg ggaggggctt tcccgtttca gcgtcccgct gaaatcggcg
    69361 gcttacctcc tttcgccatg caagcagtct atccctaacg cgtagataaa atagtttcct
    69421 gtgttattac tggatgcgtg ctcgcaaatg tgcccgtcat tcagacaatt cccgacagtg
    69481 tttcataatt cctccatttt tctcccttat tggctggcta cactagtatc attccgcgaa
    69541 acgtttcagg aagagaaact cttaacgatg aaaggcagtt ataaatcccg ttgggtaatc
    69601 gtaatcgtgg tggttatcgc cgccatcgcc gcattctggt tctggcaagg ccgcaatgac
    69661 tcccggagtg cagccccagg ggcgacgaaa caagcgcagc aatcgccagc gggtggtcgc
    69721 cgtggtatgc gttccggccc attagccccg gttcaggcgg cgaccgccgt agaacaggca
    69781 gttccgcgtt acctcaccgg gcttggcacc attatcgccg ctaatactgt tacggtgcgc
    69841 agccgcgtgg acggtcaact gatggcgtta catttccagg aaggccagca ggtcaaagca
    69901 ggcgatttac tggcagaaat tgaccccagc cagttcaaag ttgcattagc acaaacccag
    69961 ggccaactgg caaaagataa agccacgctt gctaacgccc gccgtgatct ggcgcgttat
    70021 cagcaactgg caaaaaccaa tctcgtctcc cgtcaggagc tggatgccca acaggcgctg
    70081 gtcagtgaaa ccgaaggcac cattaaggct gatgaagcaa gcgtcgccag cgcgcagttg
    70141 caactcgact ggagccgcat caccgcacca gtcgatggtc gcgttggtct caagcaggtt
    70201 gatgttggta accaaatctc cagtggtgat accaccggaa ttgtggtgat cacccagacg
    70261 catcctatcg atttagtctt taccctgccg gaaagcgata tcgctactgt agtgcaggcg
    70321 caaaaagccg gaaaaccgct ggtggtagaa gcctgggatc gcaccaattc gaagaaatta
    70381 agtgaaggca cgctgttaag cctcgataac caaatcgatg ccacaaccgg tatgattaaa
    70441 gtgaaagcac gctttaataa tcagaatgat gtgctgtttc ccaatcagtt tgttaacgcg
    70501 cgcatgttag tcgacaccga acaaaacgcc gtagtgatcc caacagccgc cctgcaaatg
    70561 ggcaatgaag gccattttgt ctgggtgctg aatagcgaaa acaaggtcag caaacatctg
    70621 gtgacgccgg gcattcagga cagtcagaaa gtggtgatcc gtgcaggtat ttctgcgggc
    70681 gatcgcgtgg tgacagacgg cattgatcgc ctgaccgaag gggcgaaagt ggaagtggtg
    70741 gaagcccaga gcgccaccac tccggaagag aaagccacca gccgcgaata cgcgaaaaaa
    70801 ggagcacgct cctgatgcag gtgttacccc cgagcagcac aggcggcccg tcgcgcctgt
    70861 ttattatgcg tcctgtcgcc accacgctgc tgatggtggc gatcttactc gccgggatta
    70921 tcggttatcg cgccctgccc gtttcggcgc tgccggaagt ggactatccg accattcagg
    70981 tggtcacgct ctacccaggt gccagcccgg atgtcatgac ctctgccgtt accgcgccgc
    71041 tcgaacgcca gttcgggcag atgtctggcc tgaaacagat gtcgtcgcaa agttccggcg
    71101 gtgcgtcagt tatcactttg cagttccagc taacattacc gctcgatgtc gccgagcagg
    71161 aagtgcaggc cgcgattaac gctgcgacca acttgttgcc gagcgatctg cctaacccgc
    71221 cggtttacag caaagtgaac ccggcagatc cgccgatcat gacgctcgcc gtcacctcaa
    71281 ccgccatgcc gatgacgcaa gtggaagata tggtggaaac ccgcgtcgcg cagaaaatct
    71341 cgcagatttc cggcgtcggc ctggtgacgc tttccggcgg tcagcgtccg gctgttcgcg
    71401 tcaaacttaa cgctcaggcg attgccgccc tcggcctgac cagcgaaacc gtgcgcaccg
    71461 ccattaccgg cgctaacgtt aactcggcaa aaggtagcct cgacggccct tcccgtgcgg
    71521 tcacgctttc cgcgaacgac cagatgcaat ccgccgaaga gtatcgccag ctaatcatcg
    71581 cctaccagaa cggcgcgcca attcgtctgg gcgatgtcgc aactgtagag caaggtgcag
    71641 aaaacagctg gctcggcgcg tgggcgaaca aagaacaggc cattgtgatg aatgttcagc
    71701 gccagcccgg tgctaacatt atctccaccg ccgacagcat tcggcagatg ctgccacagc
    71761 tcactgagag tctgccgaaa tcggtgaagg tgacagtgct ttccgatcgc accaccaata
    71821 tccgcgcatc cgtcgatgat actcagtttg aattgatgat ggctatcgcg ctggtagtca
    71881 tgattatcta cctgtttttg cgcaatattc cggcgaccat cattcccggt gttgctgtac
    71941 cgctgtcgtt aatcggcact ttcgcggtta tggtgtttct cgatttttca atcaataacc
    72001 tgacactgat ggcgttaact atcgccaccg gattcgtggt cgatgacgcc atcgtggtga
    72061 tcgaaaacat ttcccgctat atcgaaaaag gcgaaaaacc gttggcggcg gcgctcaagg
    72121 gcgcaggtga aatcggcttt accattatct cgctgatctt ctcactgatt gcggtgttga
    72181 tcccactgct gtttatgggc gatatcgtcg ggcgactgtt ccgcgaattt gctattaccc
    72241 tggcggtagc gattttgatc tcagcggtgg tgtcgctgac cctgacaccg atgatgtgcg
    72301 cgcggatgct cagccaggag tcgttgcgta aacagaaccg cttctcccgt gcctcggaaa
    72361 aaatgttcga caggataatc gccgcctatg gtcgtggact ggcgaaagtg ctgaatcatc
    72421 cgtggctgac cttaagcgtg gcactcagca cgctgctgct tagcgtgctg ctgtgggtgt
    72481 tcattccgaa aggtttcttc ccggtacagg acaatggcat tattcagggc actttgcagg
    72541 caccgcaatc cagctccttt gccaatatgg cccagcgaca acgccaggtc gcggacgtga
    72601 ttttgcagga tccggcagtg caaagcctga cctcatttgt tggcgttgat ggcactaacc
    72661 cgtcgctgaa tagtgcacgt ttacaaatca acctcaaacc gttggatgaa cgtgatgatc
    72721 gggtgcaaaa agtcatcgcc cgtctgcaaa cggcggtaga taaagtgccg ggcgtcgatc
    72781 tcttcctgca accaacgcag gatctgacta ttgatactca ggtcagccgc acccagtacc
    72841 agtttacctt gcaggccacc tcactggatg cgctcagtac ctgggtgcca cagttgatgg
    72901 aaaaactcca gcaactgcca cagctttctg atgtctccag cgactggcag gacaaagggc
    72961 tggtggcgta tgtcaatgtt gatcgcgaca gcgccagccg tctggggatc agcatggcgg
    73021 atgtcgataa tgccctgtac agtgcgtttg gtcagcgact gatttccact atttatactc
    73081 aggctaacca gtatcgcgta gtgctggagc acaacaccga aattaccccg ggcctcgcgg
    73141 cgctggatac cattcgcctg accagtagcg acggcggcgt ggtgccgtta agctcaattg
    73201 ccaaagttga acagcgtttt gcgccgcttt ccatcaacca tctggatcag ttcccggtaa
    73261 cgaccatctc ctttaacgtg ccggataact attcgctggg cgatgcggtg caggcgatta
    73321 tggacaccga aaagacgctg aatctgccgg tggatatcac cacgcaattc cagggcagca
    73381 ccctcgcctt ccagtcggcg ctgggcagca ctgtctggct gattgtcgcg gcggtggtgg
    73441 cgatgtatat cgtgctcggc attctgtacg agagctttat tcacccgatc accattctct
    73501 cgacgctacc caccgcaggg gtcggcgcgc tgctggcatt aatgattgcc ggtagcgaac
    73561 tggatgtgat tgcgattatc ggcattattt tgctgatcgg tattgtgaag aagaacgcca
    73621 tcatgatgat cgacttcgcg ctggctgctg agcgcgagca aggcatgtcg ccgcgcgatg
    73681 caatctacca ggcttgtctg ttgcgttttc gtccgatcct gatgaccacc ctggcggctc
    73741 tgcttggcgc gctgccgctg atgttgagta ccggggtcgg cgcggaactg cgtcgtccgt
    73801 taggtatcgg catggtcggc ggtctgattg tcagccaggt gctgacgctg ttcaccacgc
    73861 cggtgattta tctgctgttc gaccgcctgg cattgtggac caaaagtcgc tttgctcgcc
    73921 atgaagagga agcgtaagtg aagttttttg ccctcttcat ttaccgtccg gtggcgacga
    73981 ttttactgtc ggttgccatt accctgtgcg gcatactggg cttccgtatg ctgccggtcg
    74041 ccccgctgcc gcaggtcgat tttccggtga ttatggtcag cgcctcgctg cccggtgcgt
    74101 caccagaaac aatggcgtct tccgttgcca cgccgctgga gcgctcactt gggcgcattg
    74161 ccggagtcag tgaaatgacc tccagcagtt cgctcggcag cacgcgtatt attttgcagt
    74221 ttgattttga ccgggatatc aacggcgcgg cgcgcgatgt gcaggcggcg atcaacgctg
    74281 cacaaagttt gctgcccagc gggatgccca gccgtccgac ctatcgcaaa gctaacccgt
    74341 cggatgcgcc aattatgatc ctcacgctga catccgatac ttattcgcag ggtaaactgt
    74401 acgatttcgc ctcgacgcag ctggctccga cgatttcgca aatcgacggt gttggtgatg
    74461 tcgatgtcgg cggcagctca ctgcccgccg tacgcgtcgg gctgaatccg caggcgctgt
    74521 ttaatcaggg cgtctcgctg gacgacgtac gcactgccat cagcaatgcc aacgtgcgta
    74581 aaccgcaggg ggcgctggaa gatggcactc accgctggca gatccagacc aatgatgagc
    74641 taaaaaccgc cgccgaatat cagccgctga ttattcatta caacaacggc ggcgcggttc
    74701 gtctgggcga tgtggcgaca gtgaccgact ctgtacagga tgtacgtaac gccgggatga
    74761 ccaatgccaa accggctatt ttgctgatga tccgcaagct gccggaagcc aatattatcc
    74821 agacggttga cagcatccgg gcaaaattac cggagttgca ggaaaccatt ccggcggcga
    74881 ttgatctgca aattgctcag gatcgctccc ccaccattcg cgcctcgctg gaagaagtcg
    74941 agcaaacgct gattatctcg gtggcgctgg tgattctggt ggtgttttta ttcctgcgct
    75001 cgggtcgcgc aactattatt cccgccgttg cggtgccggt ttcgctgatt ggtacgtttg
    75061 cggcgatgta cctgtgcggt ttcagtctca ataatctttc gttaatggcg ctcaccatcg
    75121 ctactggttt cgtggtggat gacgccatcg tggtgctgga aaacattgcc cgtcatctgg
    75181 aagcgggaat gaaaccgttg caagccgcac tgcaaggtac tcgcgaagtc ggttttacgg
    75241 tgctgtcgat gagtctgtca ctggtggcgg tgttcctgcc gctgctgttg atgggcggat
    75301 tgccgggccg actgttacgc gaatttgccg tgacgctttc tgtcgccatt ggtatatcgt
    75361 tgctggtttc tctgacatta acgccaatga tgtgtggctg gatgctgaaa gccagcaagc
    75421 cgcgcgagca aaagcgactg cgtggttttg gtcgcatgtt ggtagccctg caacaaggct
    75481 acggcaagtc actgaaatgg gtgctcaatc atacccgtct ggtgggcgtg gtgctgcttg
    75541 gcaccattgc gctgaatatc tggctgtata tctcgatccc gaaaaccttc ttcccggagc
    75601 aggacactgg cgtgttgatg ggcgggattc aggcggatca gagtatttcg tttcaggcga
    75661 tgcgcggtaa gttgcaggat ttcatgaaaa ttatccgtga cgatccggca gtggataatg
    75721 tcaccggctt tacaggcggt tcgcgagtga acagcgggat gatgtttatc accctcaagc
    75781 cacgcgacga acgcagcgaa acggcgcagc aaattatcga ccgtctgcgc gtaaaactgg
    75841 cgaaagaacc gggggcgaat ctgttcctga tggcggtaca ggatattcgc gttggtgggc
    75901 gtcagtcgaa cgccagctac cagtacacgt tgttatccga cgacctggcg gcactgcgag
    75961 aatgggagcc gaaaatccgc aaaaaactgg cgacgttgcc ggaactggcg gacgtgaact
    76021 ccgatcagca ggataacggc gcggagatga atctggttta cgaccgcgac accatggcac
    76081 ggctgggaat cgacgtacaa gccgccaaca gtctgttaaa taacgccttc ggtcagcggc
    76141 aaatctcgac catttaccag ccgatgaacc agtataaagt ggtgatggaa gtggagccgc
    76201 gctataccca ggacatcagt gcgctggaaa aaatgttcgt tatcaataac gaaggcaaag
    76261 cgatcccgct gtcgtatttc gctaaatggc aaccggcgaa tgccccacta tcggtgaatc
    76321 atcagggatt atcggcggcc tcgaccattt cgtttaacct gccgaccgga aaatcgctct
    76381 cggacgccag tgcggcgatc gatcgcgcaa tgacccagct tggggtgcct tcgacggtgc
    76441 gcggcagttt tgccggcacg gcgcaggtgt tccaggagac gatgaactcg caggtgatcc
    76501 tgattatcgc cgccatcgcc acggtgtata tcgtgctggg tatcctttac gagagttacg
    76561 tacatccgct gacgattctc tccaccctgc cctcggcggg cgttggagcg ctgttggcgc
    76621 tggagctgtt caatgccccg ttcagcctaa tcgccctgat agggatcatg ctattaatcg
    76681 gcatcgtgaa gaaaaacgcc attatgatgg tcgattttgc gcttgaagcc caacggcacg
    76741 gtaacctgac gccgcaggaa gctattttcc aggcctgtct gctgcgtttt cgcccgatta
    76801 tgatgaccac cctggcggcg ctgtttggtg cgctgccgct ggtattgtcg ggcggcgacg
    76861 gctcggagct gcggcaaccc ctggggatca ccattgtcgg cggactggta atgagccagc
    76921 tccttacgct gtataccacg ccggtggtgt atctcttttt cgaccgtctg cggctgcgtt
    76981 tttcgcgtaa acctaaacaa acggtaaccg agtaaatgac agatcttccc gacagcaccc
    77041 gttggcaatt gtggattgtg gctttcggct tctttatgca gtcgctggac accaccatcg
    77101 taaacaccgc ccttccctca atggcgcaaa gcctcgggga aagtccgttg catatgcaca
    77161 tggtcattgt ctcttatgtg ctgaccgtgg cggtgatgct gcccgccagc ggctggctgg
    77221 cggacaaagt cggcgtgcgc aatattttct ttaccgccat cgtgctgttt actctcggtt
    77281 cactgttttg cgcgctttcc ggcacgctga acgaactgtt gctggcacgc gcgttacagg
    77341 gcgttggcgg cgcgatgatg gtgccggtcg gcagattgac ggtgatgaaa atcgtaccgc
    77401 gcgagcaata tatggctgcg atgacctttg tcacgttacc cggtcaggtc ggtccgctgc
    77461 tcggtccggc gctcggcggt ctgctggtgg agtacgcgtc gtggcactgg atctttttga
    77521 tcaacattcc ggtggggatt atcggtgcga tcgccacatt gctgttaatg ccgaactaca
    77581 ccatgcagac gtggcgcttt gatctctccg gatttttatt gctggcggtt ggcatggcgg
    77641 tattaacccc tggcgctgga cggcagtaaa ggtacaggtt tatcgccgct ggcgattgct
    77701 ggcctggtcg cagttggcgt ggtggcactg gtgctttatc tgctgcacgc cagaaataac
    77761 aaccgtgccc tgttcagtct gaaactgttc cgtactcgta ccttttcgct gggcctggcg
    77821 gggagctttg ccggacgtat tggcagtggc atgttgccct ttatgacacc ggttttcctg
    77881 caaattggcc tcggtttctc gccgtttcat gccggactga tgatgatccc gatggtgctt
    77941 ggcagcatgg gaatgaagcg aattgtggta caggtggtga atcgctttgg ttatcgtcgg
    78001 gtactggtag cgaccacgct gggtctgtcg ctggtcaccc tgttgtttat gactaccgcc
    78061 ctgctgggct ggtactacgt tttgccgttc gtcctgtttt tacaagggat ggtcaactcg
    78121 acgcgtttct cctccatgaa caccctgacg ctgaaagatc tcccggacaa tctggcgagc
    78181 agcggcaaca gcctgctgtc gatgattatg caattgtcga tgagtatcgg cgtcactatc
    78241 gccgggctgt tgctgggact ttttggttca cagcatgtca gcgtcgacag cggcaccaca
    78301 caaaccgtct ttatgtacac ctggcttagc atggcgttga tcatcgccct tccggcgttc
    78361 atctttgcca gagtgccgaa cgatacgcat caaaatgtag ctatttcgcg gcgaaaaagg
    78421 agcgcgcaat gaagttctgg cgacccggta ttaccggcaa actgtttctg gcgattttcg
    78481 ccacctgcat tgtcttgctg atcagtatgc actgggcggt gcgtatcagt tttgagcgtg
    78541 gctttattga ttacatcaag catggtaatg aacagcgatt acaactgtta agtgatgcgc
    78601 ttggcgagca gtatgcgcag catggcaact ggcgcttcct gcgcaacaat gatcgctttg
    78661 tctttcagat cctgcgttca tttgaacacg ataattcgga agataaaccc ggcccgggta
    78721 tgccaccgca cggctggcgt acccagttct gggtggttga tcaaaacaac aaagtgctgg
    78781 ttggtccgcg agcgccgatt ccacctgacg gtacacggcg acccattctg gtcaacggtg
    78841 cggaagttgg cgcggtgatc gcctcccccg ttgagcggtt aacgcgcaat actgatatca
    78901 atttcgataa acaacagcgg caaaccagct ggttgattgt cgccctggca acgttactcg
    78961 cggcacttgc cacttttctg ctggcgcgcg gtttactggc accggtaaaa cgacttgtcg
    79021 atggcacgca caaactggcg gcgggcgatt tcactacccg cgtaacgccc accagtgaag
    79081 atgaactggg caaactggcg caagacttca accagcttgc cagcacactg gagaaaaacc
    79141 agcaaatgcg gcgcgatttt atggccgata tttctcacga actgcgtacg ccattagcgg
    79201 tgctgcgcgg tgaactggaa gccattcagg atggcgtgcg taaattcacg ccggagacgg
    79261 tggcgtcttt acaggcggag gtcggtacac tgaccaaact ggttgacgat ctccatcagt
    79321 tgtcgatgtc tgatgaaggc gctctcgcct atcaaaaagc accggtagat ttgatcccac
    79381 tgctggaagt ggcgggcggc gcatttcgcg aacgattcgc cagtcgtggc ctgaaactgc
    79441 aattttccct gccagacagt attaccgtat ttggcgatcg cgaccgttta atgcagttat
    79501 tcaataattt acttgaaaac agcctgcgct atactgacag cggcggcagc ctgaaaatct
    79561 ctgccgagca gcacgacaaa acggtgcgcc tgacctttgc cgacagtgcg ccaggtgtca
    79621 gtgacgagca gctacaaaaa ttatttgaac gtttttatcg caccgaaggt tcccgcaacc
    79681 gcgccagcgg tggttccggg ctggggctgg cgatttgcct gaacattgtt gaagcacata
    79741 atggccgcat tattgctgcc cattcgcctt ttggcggggt aagcattaca gtagagttac
    79801 cgctggaacg ggatttacag agagaagtat gaccgagtta ccaatcgacg aaaacacacc
    79861 acgtattttg atcgtggaag atgaaccgaa gctggggcag ttgctcattg attatctgcg
    79921 tgctgcgagc tatgcgccga cgcttatcag ccacggcgat caggtactgc cgtatgtgcg
    79981 ccagacacca ccggatctga tcctgttgga tctgatgctc cctggcaccg atggcttgac
    80041 gctgtgccgg gaaattcgtc gtttttctga cattcctatc gtgatggtga cggcaaaaat
    80101 cgaagagatc gaccgcctgc tcggactgga gattggcgca gatgactaca tctgcaagcc
    80161 gtacagccca cgggaagtgg tagcgcgcgt caaaaccatt ttgcgccgtt gcaaaccgca
    80221 gcgcgaattg cagcaacagg atgccgaaag cccgttgatt atcgacgaag gtcgttttca
    80281 ggcttcatgg cgcggtaaaa tgcttgacct gacacctgcg gaatttcgtc tgttgaaaac
    80341 gctctctcat gaaccaggaa aagtgttctc ccgcgagcaa ttgctcaatc atctttatga
    80401 cgactaccgc gtagtaaccg accgcaccat tgacagccac attaaaaacc tgcgccgcaa
    80461 gctggaatct ctcgacgccg aacagtcatt tatccgcgcc gtttacggcg ttggttaccg
    80521 ctgggaagcc gacgcctgcc gcatcgttta gttttagcga catgactttg ttagccggag
    80581 atggcgttca gaccagatcc ggcaacatta tcccacgcat ggtcagcaaa ctgacattat
    80641 ctctccgccc tggcaggcat cagaaaatcg ctcatacttt aatcggtaaa cagcaccttt
    80701 agatgctgtt ttcgatacgc aatttcaatc aaggagtcat tatggctggt tggtttgaac
    80761 tcagtaagag cagtgataat cagttccggt ttgtgctaaa agcgggcaat ggtgagacta
    80821 tcctcaccag cgagctgtat acatcaaaag cctctgcgga aaagggcatc gcgtcagtgc
    80881 gtagcaacag cccgcaagaa gaacgctatg agaaaaaaac ggcaagtaac ggcaaattct
    80941 atttcaatct gaaagccgcc aatcatcaaa ttatcggctc cagccagatg tacgccaccg
    81001 cgcaatctcg tgaaaccgga attgcctccg ttaaagccaa tggcacaagc cagacggtga
    81061 aagacaatac gtaattacga tgccgggcgt gggttgttgg cgtccggctt tattttctct
    81121 catcacagag ttactatccc tcaagaaatt attaaccatg aaatgagttt tctcctcata
    81181 actgataagg aaaattatgc tcaaaggatt gcgtacatta ttttcacgca gggttttgtt
    81241 taacgtcgaa atgaataatt ctgttgactg tgaattactt ctcatccagc aacaactcca
    81301 ggcattgata gccgaaccag gcaattattt agcacatgat tgtgatctca acgatactct
    81361 ggttctttgc cagcttaccc atcatcacaa tggatatata cgagaaaagg ccgtgcagta
    81421 tctggggcac aaagaggatg ttgcggctat agaagaatta ttacttgcag ctaatgactg
    81481 ggtaaaagag gttcgccttg ctgcaaccat agcattacgt catttaatga aggataagaa
    81541 tgcagcggca tttgccgtta atctacccat gatacgtaac cttctccaat gccagcgtta
    81601 cgatcaccgt gcatttgtag acgagatact tagatattta ttgacagaaa ataaccgccc
    81661 acaattaacg tattggctta cttgccctga taaaaagctc tcccgcgcgg tattacaaac
    81721 gctgattgaa tacggttact ttaatgatga atcttcactg ctttcaatac tcgataaacc
    81781 cgatgagggt ttgcgaatgc tggcgaccac ccactggctg cgtcaacaat taccattaag
    81841 cgaagcggta ttaactcgtc tgttaaaaga ccgctggccg cgtattcgac aagcaacgtt
    81901 atttagcctc actgaccgag caatagaaat gcctccggcg ttgcacagca cattactgct
    81961 ggataacaat atgttgatcc gcctacgcgc taaaaatatg cttcacgaag tcatggatgt
    82021 tccgcaattc tggcgtcatg ttgtcacttc tgcggaatat actccctcac agcgacgggc
    82081 cgctttatac gggctggatt ccattcatga tcccaatatt ttaaaactgg cagaatgggg
    82141 attatcacag aatgtattcc cactgcgttt agccgctatg cacattcttg caaaagctaa
    82201 tcctcgttgt ggcgtcaaag agactattct gaccacgctg gctaacccgg acgccgccgg
    82261 gttgcgattt atggttaaca tctgtgtatg gtgtcgcgtt ccgctcacgt tcgaggaaat
    82321 acgtcagttg caggaaaacg cgccgtcagt taaacatgct tgcgcatatt gtcggctgta
    82381 tcataacttg aacaaatggg acggtttaat tcttcttctt caatcgcaac acaagctaac
    82441 tgaagaattt gcaggtaaac agctcgcgat atggcaacgc aatttcaatc tttcgggcat
    82501 tcaaccgaat gctcttcagc gtcagcaact tcaggcgctt tttacccgca atccggaact
    82561 tcacaacaga ttatggggtt acattccttt taaataactt cgaattatag acttacgatg
    82621 acaaatgtac ctgctgacga gcggaaccaa aacgaaacaa tacgtgaagc tttacgcttg
    82681 ctacgagaaa aacttgccga gggactacgc agtggcgaag cccaaccgtg ggaaaaggat
    82741 ctgtttttac ataaagtgaa ggccggtaac aaataaatcc cccagtcttt tccctcacat
    82801 ttgcactgga gccaaccccg ccgattgcgc tacaatgccc gcccttaaag tgggggcact
    82861 cccctaaccg cttcatcagg tgaagcggat ctgacctgtc atcagaacga gagaattatg
    82921 tttaaaccgg aactcctttc cccggcggga acgctgaaaa atatgcgtta cgctttcgct
    82981 tatggcgcag atgctgttta tgcgggccag ccgcgttact ccctgcgtgt gcgcaacaac
    83041 gaattcaacc acgaaaatct tcagctcggc atcaatgaag cccacgcgct ggggaaaaag
    83101 ttttatgtcg tggtcaacat tgcaccgcac aacgccaagc tgaaaacctt tatccgtgac
    83161 ctgaaaccgg tggtggaaat ggggccggat gcgctgatta tgtccgatcc agggctgatt
    83221 atgctggtgc gtgagtactt ccctgaaatg ccgatccacc tctcggtgca ggctaacgcc
    83281 gtaaactggg cgacggtgaa attctggcag caaatgggcc tgacccgcgt gatcctctct
    83341 cgcgaactgt cgctggaaga gattgaagag atccgcaatc aggtgccgga tatggagatc
    83401 gagatcttcg ttcacggtgc gctgtgcatg gcctactccg gtcgctgcct gctctctggc
    83461 tatatcaaca agcgcgatcc gaaccagggt acctgcacca acgcctgccg ctgggagtac
    83521 aacgtccagg aagggaaaga agatgacgtt ggcaacatcg tacacaagta cgagccgatc
    83581 ccggtgcaaa atgttgagcc gacgctaggt atcggcgcgc caaccgacaa agtgtttatg
    83641 atcgaagaag cccagcgtcc gggcgagtat atgaccgcgt ttgaagatga gcacggcact
    83701 tacatcatga actcgaaaga tctgcgcgcc atcgcccatg tagaacgcct gaccaaaatg
    83761 ggcgtgcatt cgctgaaaat cgaaggccgt actaaatctt tctactattg cgcacgcacc
    83821 gcacaggttt atcgtaaagc tatcgatgat gccgctgcgg gcaaaccgtt cgataccagc
    83881 ctgctggaaa ctctggaagg tctggcgcat cgtggctata ccgaaggttt cctgcgtcgt
    83941 catactcacg acgattatca gaactacgaa tacggttatt cagtttctga ccgccagcag
    84001 tttgttggtg agtttaccgg tgagcgcaag ggggacctcg cagcggtagc ggtgaaaaat
    84061 aaattctccg ttggcgacag ccttgagctg atgacgccgc aaggcaacat taactttacc
    84121 cttgagcaca tggaaaacgc caaaggtgta gcgatgccgg tcgcaccagg cgatggttat
    84181 actgtgtggc tcccggtccc gcaggatctt gaactaaatt acgcgctgct gatgcgtaat
    84241 ttctccgggg aaaccacgcg taacccacac ggtaagtgat taatttcgat tatttttccc
    84301 ggatggaaaa ttcttagaaa ccgatcacat acagctgcat ttattaaggt tatcatccgt
    84361 ttcgctgaaa aacataaccc ataaaatgct agctgtacca ggaaccacct ccttagcctg
    84421 tgtaatctcc cttacacggg cttatttttt atgcataagc cctatccctg gtcaccgtct
    84481 tccattgacc acatcgatag aatctccctt catagcacga tgcctttcac gtaacggcat
    84541 cgtgctcgca caggttccgg ctaagcacaa ccagaacgcg catgtttgat gcttaccaaa
    84601 aaatattctc actctccaca tttgaatgtc agacgagcga caccatgtaa tcctacacct
    84661 tctgtcttca gctcaactat ttgcattttt ttgccctgag taacacagaa atgagttgca
    84721 tcatttttta ctatattttc tgcaccagat attctactcc tggctaaaga agcttcggct
    84781 tcggtgtagt attggttatc gagtttacgc tgaatattac ttttatatgc aagaccaaat
    84841 ttaccgatac ttgtctcatc attatgcaca gcacaaccag acataataaa aatactaatt
    84901 aatgatatag cagctatctt tttcatctca cctttcccca ttaaatacca acgacactct
    84961 ctagtgttta aatataataa tagctgatca ttatatttga ataggattat aataaatgtt
    85021 ctgtacaaca tttcctacat aagtaggact tacagacatg aaggcccttt ccaggtaact
    85081 ccatggggct ttaatatatt tggaatagta tatttaagat gccactgttt ggttgaatat
    85141 taggcatatg ctcttttttg aaatttatcg gtggctaccg ttaatattcg ctgttcccat
    85201 tgcaagctcc tggtggtaac cactgaatcc tccatacttg aactgacttt ttatcctccg
    85261 actttcatcc tgttctgact ccaccttttc ttttctgctc tacactatct acagaccaat
    85321 cataaaggca catacgatca tggcagaatt tcccgccagc ttactgattc ttaatggcaa
    85381 aagtactgac aatctaccct tgcgcgaagc aattatgctg ttgcgtgagg aaggaatgac
    85441 gatccatgtg cgggtcacct gggagaaagg tgatgccgca cgatttgtag aggaggcccg
    85501 gaagttgggc gtcgcaacgg taattgccgg tggtggcgat ggcaccatta atgaagtttc
    85561 tacggcgttg attcagtgtg agggggatgg catacccgcg ctgggaattt tgccattagg
    85621 aaccgccaat gattttgcca ccagtgtagg gattcctgag gcactggata aggcgctgaa
    85681 actggcaatt gccggtaacg ccattgcgat agatatggcg caggtcaaca aacaaacctg
    85741 ttttattaat atggcgacag gcggatttgg gacgcgtatt accacagaaa cgccggaaaa
    85801 attaaaagcc gcgctgggtg gcgtctctta catcattcat ggcttaatgc gcatggatac
    85861 tctgcaaccg gaccgttgtg aaatccgcgg tgaaaacttt cactggcaag gtgacgccct
    85921 ggtattggta ttggtaacgg gcgtcaggcc ggtggcggtc agcaattgtg tccgaacgcg
    85981 ttaattaacg atggcttgct gcaactgcgc atttttaccg gcgatgaaat tcttccggct
    86041 ctcgtatcaa ccttaaaatc tgacgaagat aacccgagta ttatcgaagg cgcttcgtcg
    86101 tggtttgata tacaagcccc acacgaaatc acttttaatc ttgatggcga accgttgagc
    86161 ggacaaaact ttcatattga aatacttccg gcggcgttgc gttgtcgatt accaccagat
    86221 tgtccattat tgcgttaatc aaactattct ctcttgaacc agggagaatc cgaaatgccg
    86281 gatgcggtgt aaatacctta tccggcatac agaacaatac tacaccgtaa ttaattttac
    86341 ccgtgtcttc tgcaatgcca gtttatccac ctctgataca gcatcatcgg taataataat
    86401 gtcaattttt tcgattggca gtacctgatt aaaaccgcga cggttaaatt tcgatgaatc
    86461 gagaacggca atcactttat tagcggctgt gaccatcgca ccgctaacgg aataaccctc
    86521 attaaaggtc gtaataccat taaccgcatc aataccatca gcaccgacaa acattaaatc
    86581 ggcattaata tcctgcaggg aacgttcagc aatagatcca tgcatagatc gcgttttatg
    86641 gcggaccgtg ccaccgcaga cgaccagagt aatgtcttta ttttcggaaa gggcaaacgc
    86701 tgccgggaga ctgttggtaa tcaccgtgat atttttagcg gtcattaatc cttcggcgat
    86761 aagcattgtg gtacttccgc tgtcgagaat aacggtcatc ccttcatgga tcattttgac
    86821 tgccgcctgc gctattctgt tttttggcgc gctggcgagc tgaaatcgct ctttaaaccc
    86881 gacttcctgg gtctcggttt cactattacc ggacattatt ttcgccgcac cgccatgaaa
    86941 gcgcgtaacc acgccttttt gttcgagaaa gcgcaaatcg gcacggattg tcgcttccga
    87001 ggcagcaaat actcccgcca gatcctgaac aagcacggtt ccctgttcat tcactaattg
    87061 gatgatctta ttccttcgct cgaatgaatt catcgtcatt tacccatatt caattgtggc
    87121 tagtgtaaac gaagggggac cattggtgaa tgatctgttt tgcacaatac cgggccagca
    87181 tcacgctggc ccgcggtttc atggaatgag caacactttg cccggcatgg cattacgagc
    87241 gatgtcacgc accgcctggg tgaagctttc aaagcttcca cggtgagcga ttaatggctc
    87301 caggcttaac ttacgttctg tcagcaaccg gctcgccgtt tcccactcct gccccggcca
    87361 agggctggaa tagttcatcc aactgccgat aaccgtcagc tctttacgca atattttgcc
    87421 aaacgttgcc gatgttaaat gcagatcctg atgcaacgtg cccaccagcg ccagttgggc
    87481 atgaggaccg gcaatctcta ccgccagttc gacagtttgc ggtacgccag ccgtctcgag
    87541 gataagctga ttaaaacgca gttcgcgtaa aacgctctgc atttgcggcg cgctcatttc
    87601 gctactgtta aatgtttgca tcgaaccgaa agattttgcc agtgccagtt tttctgagct
    87661 aatgtcgata gccgtcacac tctttgctcc cagcgcgaca gcgcactgaa tagccagcag
    87721 gccaatggtt ccggcaccaa taataataac gtttttattc tcacaacctt gcgctaaatg
    87781 aaaagcatgc agaccaacgg taatcggctc aataaaagcc ccatcctcaa taggcatatc
    87841 cgtgggtaga gcaaagacat tttttcgctt aacgacaata tattcagcaa atccaccatc
    87901 acgccgcgag ccaataaaat catattttgc gcactgggaa taaaaccctt tcagacactc
    87961 tggacaagta aaacagggta ataacggcac acaggcaacc gcatcgccag ggtgtaaatc
    88021 atcaacaccg gatcccaccg catcaatata gccgctaaat tcatggccta acgttattgg
    88081 ataataatgt gcaccatttt taaatatcct gggtaaatcg gaaccacata agcccgagct
    88141 ggcaattttt acccgcacct catcctgatg tttaatttca ggaatgacgc tttctgcaac
    88201 gcgcacgata ccatcagtat cattcaccac tgatttcata aaaactcctg attgttaagg
    88261 gggataaact ccccctcagt taaaaattat tctgcgagaa cggctttctc ttgtttaata
    88321 aaaccacgcg ctctacgcca ggtaatgaaa ataccggtca gataaattgc gccgataata
    88381 atgaaaccgg gaatattttg cggggagaaa acctgaatca gtaaccaggt aatgggagaa
    88441 ccgccctgat ccattgaagc caccataccc ccggctttta acgccccagc attagccgcc
    88501 agttgggtgt gcaaaccaat agtttgcgtc gcgatccaca gagtgatgct catgatgatg
    88561 acacccgaga ttaaggtgcg gaacagattt ccacgatgca cggcgactgc catcgccaca
    88621 aagaagccga tggtggcaag atcgccaaac ggcagtacct gattacccgg aacacaaaca
    88681 gcaattaaaa tggtgagcgg gataaaaatc aggcttaccg ataccaccgc cgtatgcccc
    88741 agcagtaatg cgggatccag gccaatcagg aactcctgac cgccgaactt cgcctgtaaa
    88801 cgactacggg cctgcttagc gatgggcgtt aaaccatcca tgatgggttt aatcacccgt
    88861 ggcattagca gcatcactgc cgccgttttt accgccagct gcaatacacc tttgacatcg
    88921 taacccgcga ggatgccgat aatcagcccc atcacaaaac ccacggtgac aggttcgcca
    88981 aaagggccaa atttgcgctg aatatcatcg gcgctaaatt taattcggtt aacgcctggg
    89041 attttctcga tgatagcatc gaccagcacc gcaatcggcc ccatatacgc cgacgtaccg
    89101 tgcggaatag caataccttc cagctcaaag aaatttcggg tatcgcgggc aaaccagtcg
    89161 ccgagcttat aaacaaacgc cgcgtgaatt accacacccg ccatccctat catccatgaa
    89221 ccggttgcca gatgcagcaa cgcgccggtg aaagtcatat gccagatatt ccagatatca
    89281 acatttacca cccgcgtcat acgggtcagt aacatcgcca cgttaaccag aatagcaatc
    89341 ggaatcgcca ccagcgcaat ttgcgacgcc caggtcattg gtgaagagcc cggccagccg
    89401 acatcgacca catgcagatt caggtcgaaa ttttccgcca tcgctttcgc cgccggacca
    89461 atggaatcca gcattaagcc aatcacaagg ccaatgccaa caaagccaat ccctatatga
    89521 agacccgctt taaagcaatc gcctgccttc atgcctaata ttttagaaaa aataatgatg
    89581 acaatcggca gcatcaccgt agggccgagg tcgagaatat aacgcatgac ttctgaaaac
    89641 ataggtcacc cctgtaagat agtcagaatt ttattttgta atgcttcgat accgacacca
    89701 gaaacaaaag gcatgccgtg aactaacgga atatcgccaa aactacgatc caccctggca
    89761 gtagtgcata tcaaatgcac gccatccata taggtttcta tttcattaac ccgacactgg
    89821 attaattcaa caggaatatt atgattctga cacaattctt taatttcttc cgccgccatc
    89881 gtagaggtcg caaccgcgcc tccgcaagcg acaataatct tgcgtttcat aaatagtcct
    89941 ttttattgtt acagagggat ttattcgtct gaatctaaaa catactttgt gaagtattcc
    90001 tttaactggg tttcaggaag agtgattagt gtctcgacga tatcgggctg ttgtaacttg
    90061 ccaaataaac agcgtaaaag tttcaattgc tgctgcggat tttccacaat caacgcaata
    90121 accaacgata cctccacgtc gttatcatca tccgcttgct gaaaatgaac tttatttgtt
    90181 ggccttaaca gataaatggc tgacgactta gcatgaatcg cctcacaatg cggtattgca
    90241 atggcgtgct gctcaagcat tatcccggta gggaattctg cttctctggc aattaatgcc
    90301 tgtggccagg tatcatgaac cacacctttg gcgagcatct cattaccgat atgggttaaa
    90361 acttcgctac gatcgacaaa agaaattccg ctacgaacaa acaggttagt catataccgt
    90421 ccttattccg cacagccgta gcgataggcg cgcaaaacat cataaatttt atccataatc
    90481 agctaatgcg gtattgctga taattccccg gactgaatgc gttcaaattg tttgggaaga
    90541 tactgactaa tcatgcccag tgggatgtcc acgccttcaa gattcaccat catcgtttcg
    90601 acgctatttt taatccgact atgcggccag taataacgaa tacgatccga caggctgtaa
    90661 cgaatatcca gtaatgaatc gttaaaaccc gtacgataat attttttcca gtattgcggt
    90721 tcgtcgagca tcacttcttc aattaccgcc aggcaaccgc tgcgattttc aggggcgata
    90781 agttcctgct caatttgtgc cagtgcaaat atcgcctcgc gtaaagcaaa ggttaatgcg
    90841 ggaccgactt tcaatattgc aaagtgatcg cggactaatt cccaataagc cgtccgggtc
    90901 tggtaatcgg tagaatgtgc ttcataaacc attcgggtgt tttctatcca ttgcgccagc
    90961 ggctgcgctt cctgcggctg ataatggata atattgctgt gatcaaattc cacgcccggc
    91021 tgcaccacga tggcaatcac gcgtgttaac gcctctgtca gcccacggac aataaaggcc
    91081 ttttgatgcg tacgtaaagt attggcggca tcttcaacat gggtgatgtg tactgactga
    91141 atggcgctgg cctcaccgcc cggaaccgga acttcggtgc caatgacata gctcagttgc
    91201 tcacgctggc aatctgtcgc cacactttcc gcagcaaagc aaagcacagc agctcgttcc
    91261 gcaaccgttt ctggtgctaa cggtatggga tcccccgcgc aggacattga cgcatcaaga
    91321 tgaattttac tgaagccggc acgaacatat tcctttacca gctcgacgga tttttccatc
    91381 gccgcatccg cattttcttg ctgccagcag tttggcccca gatgatcgcc gccgagaata
    91441 atgcgttcgc gtgcaaaccc aactttatcg gcaatcgtaa aaacaaattc gcgaaagtct
    91501 gccggtgtca ttccggtata accgccaaat tgattgacct ggtttgacgt tgcttcaatc
    91561 agcactttgc gcgtgctgtt gcgatcaaat gccagcgccg cttcgataac caacggatgg
    91621 gcagaacaga ctgaacatat gccgatatgt tcaccagctt tatgccgggc aattaacgtt
    91681 ttcatgtttt ttccttatta aatggcaggt gcgttatgcc ctgccctcgc agccacaatc
    91741 ggcaatcact ttgctcacca catcgcgcat tgcggattta gccgactgca aataatcccg
    91801 gggctcggtc gcttcagggt gctcggtcag gtaatttttt aacgcctgcg agaaggcatt
    91861 tttcagctcc gttgcaacgt tgattttgca tatccccagt ttgatggttt gctgaatatc
    91921 cttagtcgat aaccctgacg cgccatgcag caccagcggt aagttcaccc actggcgaat
    91981 gttctccagt ctagaaaaat caagcgccgg tgcgctggca tacatcccat gagccgtgcc
    92041 gatggcgacc gccagggaat caattccggt tgcctcggca aattcacgcg cctgagcggg
    92101 gttggtataa aaagcatcgg cttcattgac ttgcacatca tcttcctggc cgccaagttg
    92161 ccccagctcc gcttcgacgc tgacatcaaa gcgatggcaa aaatccacca cctctttgac
    92221 ccgtgaaata ttttgcgcaa aaggcaaatg cgaggcgtca atcatgactg agcgcacgcc
    92281 agaacgaacc ttctgagcga tatcgtcaaa tttcgtgtga tggtcgagat gaattgccag
    92341 tggatggtga tattgcttcg ccatcgcgct gaccagcgcc aacagatttt ctgtaccagc
    92401 atgagtaaat gtgccaggcg ttccggcgat gatgaccggc gcatgcaggt tggcagcggt
    92461 ttccaccacc acttgcatcg tttcgagatt gtgaatattg aatgccggaa ccgcataacc
    92521 gccgcgctgt gcgttgttca gcatctgctt tgtcgatacc acgtgcattt tcatatcctg
    92581 tcgtttgttt tcgttttcaa aatataatga aattatttgt ttttaaatat cgagataacg
    92641 atcacaaaaa cgacaatatg aaaattattc gaggagtgaa aggcaaaaaa acggcctccc
    92701 gatagggaag ccgtagcaaa gtgcgcgtgt ttttatgccg gatgcggtgt aaacatacgt
    92761 ggcagtgcta tgtaggcatg ataagacgct tccgcgtcgc atcaggcata ggttgccgga
    92821 tgcagtgtaa acgccttatc cgtcctacgg ggtggtgttg ttcgtataag gcgtatcagg
    92881 caatagtaat tttgctatcg agataaacgt cctgcacggc gttaatcagt ttcacgccgt
    92941 cagccatcga tttcttgaac gccttacgtc caagaatcag ccccattccg ccagcgcgtt
    93001 tgttgataac cgcagtacgc actgcatcac tgaggtcagt ttcaccgcct gcagcaccgc
    93061 cggagtttat caacccagcc cggcccatat agcagttagc taactgataa cgcaccagat
    93121 caatcgggtt ttcgctggtt aacttgctgt acacgcgatc gtcggtataa ccgtaattaa
    93181 tcgctttata gccgccgtta ttttccgcca ttttttgttt gacgatatct gcgccgatgg
    93241 ttgccgccag atggtttgcc tgaccggtca ggtcggcgga aacatggtaa tcaacgccat
    93301 ctttcttaaa ggcggagtta cgcaaatagg cccacagcac ggtcaccatg cccagctcat
    93361 gcgcacgttc aaaagccgca gaaatttctt caatctggcg acgtgactct tccgagccaa
    93421 aatagatagt cgcaccaacc gccaccgcgc ccatgttgaa agcctgctcc acgctggcat
    93481 acagcgtttg atcgtaggta ttcgggtaac ttagcgtctc gttgtgatta agtttgacga
    93541 gaaatggaat gcgatgcgca tagcgccgcg ataccgacgc caacacgccg taagttgata
    93601 ccacacagtt acagcccgct tcgatcgcca gttcaacaat gtttttcggg tcaaagtaga
    93661 gcgggttagc agcaaatgaa gctccggcag agtgctcaac gccctggtca accggcagaa
    93721 tagaaagata tcctgtgcca gccagacgcc cggtgttgta caacgtctgc atattacgta
    93781 acaccgctgg cgggcgatta ttgtcaatca ttacgcggtc tacgtagtca tgtccgggga
    93841 gataaagctg gtcagaagga attgtcatac aacggtgctg taaaaggtta tcggcgtctt
    93901 tgccaagcaa ctgcgcaata tctgtcatta ctatgctccc gtaaattccg attggatatc
    93961 ggctatggat tgtcctggcc cgccttttgc gggcaatcat aatcctggtc gttacgggcg
    94021 agtttttcca tcttttggat gttttttcag cgttttctgt tggctcgatt catcagaaaa
    94081 aatgttagcg cggtcaagtt ttcaccgtaa agatatttaa aaggtattat taagtggtat
    94141 tgtcatcgcg taccttacat tacctgtcat gaaggaatta aaagatgaaa acaacagcaa
    94201 agctgtcgtt catgatgttt gttgaatggt ttatctgggg cgcgtggttt gtgccattgt
    94261 ggttgtggtt aagtaaaagc ggttttagtg ccggagaaat tggctggtcg tatgcctgta
    94321 ccgccattgc ggcgatcctg tcgccaattc tggttggctc catcactgac cgctttttct
    94381 cggcgcaaaa agtgctggcg gtattgatgt tcgcaggcgc gctgctgatg tatttcgctg
    94441 cgcaacagac cacttttgcc gggttctttc cgttactgct ggcttactct ctaacctata
    94501 tgccgaccat tgcgctgact aacagcatcg cttttgccaa cgtgccggat gtggagcgtg
    94561 atttcccgcg cattcgtgtg atgagcacta tcggctggat tgcctccggt ctggcatgtg
    94621 gtttcttgcc gcaaatactg gggtatgccg atatctcacc gactaacatc ccgctgctga
    94681 ttaccgccgg aagttctgct ctgctcggtg tgtttgcgtt tttcctgccc gacacgccac
    94741 caaaaagcac cggcaaaatg gatattaaag tcatgctcgg cctggatgcg ctgatcctgc
    94801 tgcgcgataa aaacttcctc gtctttttct tctgttcatt cctgtttgcg atgccactgg
    94861 cgttctatta catctttgcc aacggttatc tgaccgaagt cggcatgaaa aatgccaccg
    94921 gctggatgac gctcggccag ttctctgaaa tattctttat gctggcatta ccgtttttca
    94981 ctaaacgctt tggtatcaaa aaggtattat tgcttggtct ggtcaccgct gcgatccgct
    95041 atggcttctt tatttacggt agtgcggatg aatatttcac ctacgcgtta ctgttcctcg
    95101 gtattttgct tcacggcgta agttacgatt tttactacgt taccgcttac atctatgtcg
    95161 ataaaaaagc ccccgtgcat atgcgtaccg ccgcgcaggg gctgatcacg ctctgctgcc
    95221 agggcttcgg cagtttgctc ggctatcgtc ttggcggtgt gatgatggaa aagatgttcg
    95281 cttatcagga accggtaaac ggactgactt tcaactggtc cgggatgtgg acttttggcg
    95341 cggtgatgat tgccattatc gccgtgctgt tcatgatttt tttccgcgaa tccgacaacg
    95401 aaattacggc tatcaaggtc gatgatcgcg atattgcgtt gacacaaggg gaagttaaat
    95461 gaaaacagaa cgtattctcg gtgctcttta tgggcaggcg ttaggggatg cgatggggat
    95521 gccctccgag ctttggccac gcagccgcgt taaagcacac tttggctgga ttgaccgttt
    95581 tcttcctgga ccaaaggaga ataacgcggc ctgttatttt aaccgcgccg aattcaccga
    95641 cgatacctcg atggcgctgt gtctggcgga tgcgttactg gaacgtgaag gcaagatcga
    95701 tccggatctg attgggcgta atattctcga ctgggcgctg cgtttcgacg cctttaacaa
    95761 aaacgtacta ggtccgacct cgaagattgc gcttaacgcc attcgcgacg gtaaacccgt
    95821 tgctgaactg gaaaacaacg gcgtgaccaa cggcgcagcg atgcgcgtct cgccattagg
    95881 ttgtttgctt ccggcgcgtg atgttgattc ctttattgat gatgtagcgc tggcgtccag
    95941 cccgacccat aaatccgatc tggcggttgc aggcgcggta gtcatcgcat gggcgatttc
    96001 tcgtgccatt gacggagaaa gctggtcagc gattgttgat tcactgcctt caattgcgcg
    96061 acatgcccag caaaaacgca ccaccacgtt cagcgcctca ctggcggcgc ggctggagat
    96121 tgcgctgaaa attgtgcgca atgccgacgg caccgaatcc gccagcgaac agctttacca
    96181 ggtcgttggc gcaggtacca gcactattga gtccgttccg tgcgccattg cgctggttga
    96241 actggcacaa accgacccga atcgctgcgc cgtcctgtgc gctaaccttg gcggcgacac
    96301 agacaccatc ggtgctatgg cgacggcaat ttgcggcgcg ttgcatggcg ttaacgctat
    96361 cgatcctgcg ttaaaggcgg aactggatgc ggtaaatcag cttgatttca accgctatgc
    96421 cacagcgctg gcgaaatatc gtcaacaacg ggaggcggta tgagcggcgc tcgattacac
    96481 acgctgctgc ctgaattaac cacgcgtcag ccggtgatgg tcgtcggcgc ggcggtcatt
    96541 gacgtgatcg ccgacgctta tgccctcccc tggcgtgggt gcgatatcga actgaaacag
    96601 cagagcgtta acgttggcgg ctgcgcactg aatattgccg tggcgttaaa gcgcctcggc
    96661 atcgaagcgg gtaatgcctt gccgctcggt cagggcgtgt gggcggagat tattcgcaac
    96721 cggatggcaa aagagggctt aatcagcctg atcgataacg ccgaaggtga taacggctgg
    96781 tgtctggcgc tggttgagcc ggatggcgaa cgcactttta tgtcattcag cggtgtggaa
    96841 aatcagtgga atcgccagtg gctggcgcga ttaaccgttg cgcctggcag cctgctctat
    96901 ttttccggtt atcaactggc ctcgccctgc ggcgaattgt tagtggaatg gctggaagag
    96961 ctgcaagacg tgacgccgtt tatcgatttt ggcccacgta ttggcgatat cccggatgca
    97021 ttactggcgc ggatcatggc ctgtcgacct ttagtgtcgc tcaatcgtca agaggctgag
    97081 attgccgccg aacgttttgc tttatccgca gagataacaa cacttggcaa gcaatggcag
    97141 gagaaatttg ccgcgccgtt gatcgttcgt ctcgataaag aaggcgcatg gtatttcagc
    97201 aacgacgctt ctggctgcat tccggcattt ccaacgcaag ttgtcgacac cattggggcg
    97261 ggcgacagtc atgccggtgg cgtacttgcc gggctggcct ctggtctgcc actggcggat
    97321 gccgtattac tgggcaatgc agtggcgtcg tgggttgtcg ggcatcgggg cggtgattgt
    97381 gcgccaacgc gcgaggaact actcctcgca cacaaaaacg tatagatcgc tgcgacagtg
    97441 gctaatgctg tactcaatag gccgttgctg ttggtcaagc gccacttgct tgatcaccag
    97501 caccggtatt ttgctatcta actgaatatg tgactgaaac tcggcatccg gcatccgggc
    97561 gctaacgcgg gaacgcgtac gttgtgggta aatgtgctgg ctgcagaaat agtcataaag
    97621 tgaaatcccg atggcatcaa catcatgaat taaatgcgcc ggaacccacg attcctcaat
    97681 cgataccgct tcttcatcga catagcgaat acgcttaagc aaaaagacat cacttcctgc
    97741 ttcgaccgcc agctgctgcg cgacttcttc gggacatttc acgacacgct tgttaaccca
    97801 tagcgtatcg ggctttttcc cgcgcaatac cacctgctga gaaaaacctc gcgcttcttt
    97861 caacgagtat tcgaagatgt tgttgatctg cgtgccgtaa ccgcgcgaac gcgtcaccac
    97921 accctcttct tccagcgcct gcatcgcctt gcgcaccgta atgcgcgaaa cgccggttaa
    97981 ctgacttaaa tcacgctcac cgggcaaaat attgccatgc tccagcaccc cgctgcgcac
    98041 ggcatttttt accgtttcgg caaacttaat ataaagcggc gtgttatccg ccgctaaaat
    98101 acgctcgttc agttgggcaa tcagctgggt atgcgcttgt tccatttgtc tttttcctgc
    98161 cggggagtct ggtgggagta tatcactgct tcttatttaa ggcgtttctt taatcccatc
    98221 aacgaatgcc tgcaactcct ccaccgtgcc attaaataca ttaaaatcca ccggaccatt
    98281 gatgccatct acctgtccac ggtcggaatg ctgccagaag cgccaggcca tgccgtcatt
    98341 gtccggacga cgttgataat agtgagccac ccaccacgga tattcattga aatagcccgc
    98401 cagattcgtg tgataaaaaa cggctcctga gtaaataatc ggctttttcc ccgtactttt
    98461 ttcgaccatt tttagccact gacttaccct cttgcgtaat tcttttgccg ataattttcc
    98521 tcgctcttct acgtccagta ccgcagggaa atcgccttgt gagaaatcta ccgtttgcag
    98581 aaataatcgc acctgaaccg gtgctgctac cgacggggag aaataatgat atgccccgcg
    98641 cagcaggcca ttttcgcggc ttagttgcca gttacgcgaa aaataggggt ccaccagctt
    98701 ttcgccttcc gtcgccttaa taaaagcaaa ctgtaagcgg ataccgttgt cgcgcatttt
    98761 tgccacccgc tgccagtcga tccgctcctg ccagcgcgaa acatctattc cgtgaatggt
    98821 gtaactggca ggaatgcgaa tagcgaaaga tttaaccggg cgataaccgt agaagtggat
    98881 gtagtctgta atctgaactg ctgtcgaata gaaaaaattc accgtctgac gcggataaat
    98941 tgcaacgatg gaaattagcc caagagcgca taatagagag gtgagttttt ttcgactggt
    99001 aattcttagc tgcattggtt tcatccctga atgtcagtgc caaaggctga caataaccaa
    99061 agcagctata gtacggtgct caccaccagg cgtggaagtg gtgaaccgga ccaataccgt
    99121 gaccaacttc cagcgtgtcg gcctgggcta acgccgatga aagccagctt tttgcctcct
    99181 gtacggtgtc agcccagttt gtatggcgcg ggcgtagtgc agccaacgcc gcagagagtg
    99241 tacaaccagt gccgtgggtg tttttggtca taatgcgcgg tgcggtaaac cgttgttcac
    99301 cctcgcgggt aaacagccag tccgggcttt gctcatcatc cagatgacca cctttcatta
    99361 gcactgcgcc acagcccatc gccaacagcg atcgcccttg ttccagcatt tcctgttcgg
    99421 tgcgcgcgtg tggcgcgtcg agcaaggcgg cagcttcggg caagtttggc gttattaatg
    99481 aaacctgtgg caataatcga ctgcgcagcg tagcaaccgc cgaaggtgaa agcagcgggt
    99541 cgccgctttt tgccagcata acggtgtcga gtaccacgtt ttggatctga taacgttgca
    99601 accgttctgc caccgcttca acaatatcgg tttccgccag cataccgatt ttagtggtat
    99661 cgattcgcac atcgctgaac accgaatcga gctgggcggc gacaaaatca ggctcaatgc
    99721 gatacaccga ctgtacgcca cgggtatttt gcgccaccag tgcagtaata actgagcaac
    99781 cataagcgcc aagtgccgag aaggttttaa gatcggcctg aatccccgca ccaccactcg
    99841 gatcagtacc ggcaatcgtc agagcgttaa ttcgtttcat gcctgcacct cctgcgtcaa
    99901 ttgccagagc gcatcaagga aatgtggaac aaaactgcct ggcccctcgc ttctggcgac
    99961 tgcgcgttct ccggcttgtt tcatccagtg acaggcagat gcgacatttt ccagcgtatc
   100021 gcctggtaac gcacagcagg cagcgacaac cgccgataat gcacagccag ttcctaccac
   100081 tttggtcatt aacggatcac caccgtgaat accaatgata cgatgtccat cggtaacata
   100141 atccatctcg ccagtgacca cgacgattgc gccagtttcc cgtgccagtg tttgtgcagc
   100201 gggtatcgcg ttagctgcgg cgtcagtggt atccactccc cgtccgccat tagcaatgcc
   100261 agctaatgcc atgatttccg aagcattacc acgtatcgct gccggtttaa aagataaaag
   100321 ttcatgacaa aaatggcggc gataatcgag cgcacccacc gctactggat caagcgtcca
   100381 gggtgtttga gagctttttg cttgctcaac ggcagcacgc atcgcctgag cgcgtggctg
   100441 cgtcagtgtg ccaacgttaa tcaacaaggc actggcgata gccgcaaact gactggcctc
   100501 ttcggtttcg ataaccatcg ctggcgatgc accgagcgcc agcaaggtat tggcggtaaa
   100561 ggtttgcacc acatcattgg tcatgcagtg cacaagaggg gaatgttggt gaaaaaggtg
   100621 taacgcgtgc gcagattgcg ctgaacccag caggtcgact tgcatagttt gctcctgcca
   100681 taacgtgaag aagcaatgac ctggtggtcc gtgacttccc tacgctggca ttatccagat
   100741 caggtgatac gggtatttct cagccttcac gcagaagggc accccgagtc gtttggttgc
   100801 gatgataaat cgcagaggag gatggtaatg tccagcgcac gcgttgtaaa cgaggaaaaa
   100861 cggcagtttt acaatcgcgt taataaatta tttgatatat gaatccagca ccttcagaac
   100921 gacatccaga tcttcttcac gttttagctc atccccctgg tgaacgatgt gttccgtcag
   100981 atgaccttta atcacttccc gcatcagacc gtttaccgcg ccacggatag cagcaatctg
   101041 ttgtaaaact gcagcgcatt cgtgcggctc gtcgagcatt ttcttgagcg ccacgacctg
   101101 gccctgaatc ttactggcac gcgctttcag tttctgttta tcacggattg tatgagacat
   101161 ggcaacacct ggttaacaag aatatgaaaa atcatagcac tattaatcta ctggggggta
   101221 gtatcaggta ctggggggga gtagaatcag attgccgaat taatactaag aattattatc
   101281 atgaccgaat ttacaactct tcttcagcaa ggaaacgcct ggttcttcat ccccagcgcc
   101341 atcttacttg gtgcgcttca tggcctggaa ccagggcact caaaaacgat gatggcggcg
   101401 tttatcatcg ccatcaaagg caccattaaa caagcggtga tgctcggact ggcagcaact
   101461 atttcgcata ccgcagtggt ctggttaatt gcctttggcg ggatggtgat cagcaagcgc
   101521 tttactgctc aatcagcaga accgtggctc cagctgattt ccgcagtgat cattattagc
   101581 accgcgttct ggatgttctg gcgtacctgg cgcggcgaac gcaactggct ggagaatatg
   101641 cacgggcatg attatgagca tcatcatcac gatcacgaac atcaccacga ccatggacat
   101701 catcaccatc acgaacatgg cgagtatcag gatgcccatg cacgagccca tgccaatgac
   101761 attaaacgac gctttgatgg tagagaggtc accaactggc aaattttgtt atttggctta
   101821 accggtggcc ttatcccctg cccggcagca attaccgtgc tgttgatatg cattcagttg
   101881 aaagccctga cactgggcgc aacactggtc gtcagtttca gcattggcct ggcgttaacg
   101941 cttgtcaccg taggcgttgg cgcagcaatc agcgttcagc aggtcgcaaa acgctggagc
   102001 ggatttaaca ctctcgctaa acgcgccccc tatttttcca gtctgttgat tggcttagtc
   102061 ggtgtgtata tgggcgtaca tggcttcatg ggcataatgc gataactcaa tcctttaggc
   102121 ttgcatgtta tcttcagccc aggatgattc ttaaatcagc tatttccgct gactctctgc
   102181 tcgctaagga tgcttttagg gcatcctttc atttacactt tttacgaaat catgggatca
   102241 ctaacaaaat atcgcttgtc agttatattg tatggcagga aagatatgcg actgatatta
   102301 cagatcccca aagtggagag tttatgacca ttaaaaataa gatgttgctg ggtgcgcttt
   102361 tgctggttac cagtgccgcc tgggccgcac cagccaccgc gggttcgacc aatacctcgg
   102421 gaatttctaa gtatgagtta agtagtttca ttgctgactt taagcatttc aaaccagggg
   102481 acaccgtacc agaaatgtac cgtaccgatg agtacaacat taagcagtgg cagttgcgta
   102541 acctgcccgc gcctgatgcc gggacgcact ggacctatat gggtggcgcg tacgtgttga
   102601 tcagcgacac cgacggtaaa atcattaaag cctacgacgg tgagattttt tatcatcgct
   102661 aaaaaaatcc ccctcatcat gagggggaaa tgcagacacc ttgttatttt ttattattag
   102721 ccacttgctc gtcttgcttg ttattagtcg tatttcacgt tgattaatgc ggttgcctcc
   102781 aatgcgccag atttaacttt gtttgtatcg tagacgtagt aactggctgt tatcggaatt
   102841 gaaaccgttg tatctgtaag ttttgacttt gccgcaattt cttttaaatt tgtgtatcct
   102901 ttgctgttat cagtgatatt taaaagtgtg actggcgaac cattactttc aagaataaat
   102961 cctacgccat tatcttccgc acctttcagt attgtgttgc tgttggttaa caagtcagaa
   103021 cttgataaat acacctgcat tttccgtgat gtttgatttt gttgacctaa taaattttta
   103081 caacgcagaa taatatcccc ttttttacta tttattgttg ctttgttacc tgtggctggt
   103141 aattgtgaaa cgggaatacc ctctaaagta attaataaat cgtccggttt gcaggttgtg
   103201 gttatcgggt tgtatgacaa ggtcagatta aaggaataga cacctgcatt ttgtgccaat
   103261 gcatttctat agctatttac acatgcatcc catgacttgc cacctaataa acacagtaga
   103321 aattgtactg cggcactccc gcgtgtcgat gatgaagcac tggataatgt cgcaacacca
   103381 ttaatcatca ctgaaccgcc gccccccgca gaaacattca cttttgcatt actggccgca
   103441 ggtgtcaccg ttaccgaata accattggtg aaactggtct gattatttga caacgtgatg
   103501 ttatttttat tggtccattc aaaatgtaaa tttaatttaa tggtatcgtt ataaagtcca
   103561 acaataatat ccgacgggct cataggggtg atagtatttg tactcacgca tgacgaatca
   103621 ttaaaattat gctcatatgt cgccatttgt gtttgagtaa gtgcaaaatc cccctgaaca
   103681 accaagttgt gctgaacaga taactcacta taacatgccg catagctcac aggcataacc
   103741 atcatcatta atataaatag cattatgcga atttccatag aaccacctga ctattactgg
   103801 caaatataat ttctactttc atcaatctct ttaccgaagg tgattgtgca tgaaagtcct
   103861 tgttgcttat caattgctac attaaccgat ggcggtactt cattggtgcg aataaataac
   103921 tggcttccct gaccgacaac gccaatatta tgaccatgga tatcattgac ttcataacca
   103981 aacattaatg gttgcccatc cgctcttaac gcttttataa accagggctt gcgctgatcg
   104041 gtatcaaaat taaccagtac aaccgcgccg cgataagggg cggcaatttt ccggttgcca
   104101 cgtaattctg cttcgctatc gctttgcgac acatccagca tcaggtgatt ttcccgataa
   104161 ggtgtcattc cgtcgtatat caccactcca ttacggtttg ttgtgcgata tttttgccca
   104221 ttgacataag catctttaat tcctggcgca ttcatcacag caaacgtttc ggaaagacgg
   104281 ttcgccagat taacgccacc cgaccaggcg acaatgcccc ctgaaacact ggctccagcc
   104341 tgtcgataag tactcgactg actataactg ccattcactg tcgcaaccgg cgcgttccag
   104401 gtcaaattcg ccccagctgt cgtttcattt ccctgatgtt gatgactcag gttgacacca
   104461 taattgaact gatcccgact ccctactgtt cctgataatc ccgtattatt tgaggcaaac
   104521 ccctgatcat caaacgtcgt tgagttagac atatatattt gccgacgagg cgtcgtaacg
   104581 tcatcacccc aatcaaaggg aatcgatata aaaatattaa atcgtttctc ttcatgatga
   104641 ttctcgtcat aagcctggct tgccgcgagg gtatagctta tccgccgcca gttattggaa
   104701 taactcaact gataatcctt actgctgccg ctacgtcccc agtaatctcg ccataacgta
   104761 cttaaggaca cagaacccca gccttctggc aatgactggc tcatattggc agaaaagcta
   104821 tttttacggc caaaatcgtt ctggtaataa tcggcaatgt catagacatc gttttcatca
   104881 cggcgataat tatctttatt gtttgcccaa acgtgatcat taaatgtccg gtaatcacgc
   104941 gatgaataac gccaggccgc aagaccaaaa cgcgtcgacg tttggctcac aaatttgttg
   105001 taggcaattt gataactttg cccgtcaaac acatcgccgt tgtcttgttt actatgcgac
   105061 ttagtggcat cgacggaaat ggcaccaatg cgtgtattcc agccagtccc caaagtaaac
   105121 gcgtaataat tattcgcgac catcgagccg ccatacagcg tcaataaatt attaaaacca
   105181 tactgataac ccacctggac aaattcactt tgtttgctcg ccccttcaat atggctacga
   105241 cccgccgcaa aatcatattt cgacacgccg ggttgcagca tatttggcac cgctgcataa
   105301 ggcaccagat aggtggttac cgaaccgtcc gcctctttca cgctgacatc aagatctgct
   105361 ccaccaccgg ccaactgcaa atctgtaatc gcgaacgggc caggaggaac ctctttctga
   105421 taaacgacaa aaccattctg ttcaatagtt accagcgcgt tactctgagc aatcccctgc
   105481 acccgtggcg taaaattttg tttcgagtta ggcaacatct gcatatcacg aaacaaccgc
   105541 acacctctga agcgaacaga atcaaaaata tcgcttgatg tgtacatatc acccacgcga
   105601 agcgtgccga gaagttgggc aaatccacgt tccagataca gggtattgct tttccacacc
   105661 cctggattgt tatttgtttt actgaaactg gcatcagaat gcagttgcca ccccagtaaa
   105721 tttaacccgc tgttaaaacg tacatatgta ctcttgttat tacccgacgc tttatagtcg
   105781 ctgtaatact gactcagata ataagaggta taaaacgcat taataccccg ctcccagttt
   105841 tccggtggaa catagccact ttccagttct tccacccagg cctgcgggac actgaaatcg
   105901 agacgaaaaa ccccgatatc ccaggtatag ctcccaccct gaacaagttg ctcaaatgtt
   105961 aaacattgct taccgctggc gaagttatcg ctattaatgc ctaaccgctt gataacttct
   106021 cttgataaac atgtttcttg cgggttgtct ttaacaataa tctcatattt cccgcgccat
   106081 tgcttattga cataaatatc gatgtcatac tgccccggta agggttgatt atcatcaaga
   106141 cgaatatttg caacctgctg gtctttcatt ccacctatca taaaatgggt atcaaaggtt
   106201 tcttcagctg cataagcttc aatgccgagc aataacgcta cgattgctga tgcaagtggg
   106261 gtcattctca acataggcag ctcctgcaat taaattttgt cactaatata gttgccatgg
   106321 tcatcgataa tggtcagatg ccagttattt gcattattac ttttgacatt aacactctga
   106381 ctttctaagg gggcaatcat aatagtttca taattgactt tgacattatt agctttgaca
   106441 tccgaaatcg tcacccaatt agctgagtca ttttttatca ccaaaccatt gccactgcga
   106501 tttaccagca attttttaaa tgtcgcttta tttaccggag caatacccgc tggccggtaa
   106561 aacaacttaa ttctgttttg catcgcaaac ttcagtgcat tcttaccttc ttgctctgga
   106621 ctatttggtg gaatatccag aacattcaga taaaaaatgc tttctttatt agtgggcagt
   106681 ttattcggca taattttgat ttttacttgc tgcccggaat ttgcccctat ttttgccact
   106741 ggtggcgtta acatgaaagg aacctgaatt ttttctggtg gtaatgacgt atcgccatca
   106801 tcaatccacg cctgcagcag cgaagaacgg tttccctggt tcatcaactg caccatcact
   106861 tctttatttt ctgccgggta aataatgcgc gtcccgtaga taacaattcc ggcatgtgca
   106921 ggaaggacca tagaaaaaat gagtaaagat aataatcctt tcattttaat gcctcttaaa
   106981 ttaaaaggcc gccatgggat agcggccatg tttattacct ggagaaactt acaattttat
   107041 ttattaatcc tgcgccacac gcataaccag agaagataac acatcacctg attgcactga
   107101 gttatctgtc agtttcaggt attttgcttt aaacggaata gcatattgac cttccgctgt
   107161 tttataactc tcaatgttaa aaggtgcaga aacatccagg acatcgctgt tatcacctaa
   107221 atagagatag taacccacgg tatcgttgct actggctatc gccttatcgt taccggttat
   107281 tttatcgttg gtaatcaacg agagttttac accagcaact tctttcgggc agctaatttt
   107341 taacatgctg ctggttacac cgactgcatc atcataggta gaatatttag ctttaccttc
   107401 aaagacactg gttggtacag tcggcatatt aatacttact gaatcaccac ccgcttcgaa
   107461 tttacacgga gattctgcaa tattcccctt aatagttaat gttccggtat caacgtctgc
   107521 agcaaatact ccagcgctca taaaaaaaga agaaaagaca gcggcagcaa taattgaacg
   107581 tttcataata tgttccttaa taaaatttat ttttggttgt taagag
//
LOCUS       DAJVUJ010000002        79594 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.002, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000002 DAJVUJ010000000
VERSION     DAJVUJ010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 79594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 79594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..79594
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.002"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            557..1759
                     /gene="proV"
                     /locus_tag="OQQ48_000101"
     CDS             557..1759
                     /gene="proV"
                     /locus_tag="OQQ48_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HCS1951009.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            1752..2816
                     /gene="proW"
                     /locus_tag="OQQ48_000102"
     CDS             1752..2816
                     /gene="proW"
                     /locus_tag="OQQ48_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HCS1951010.1"
                     /translation="MADQNNPWDTTPAADSAVQSADAWGTPATAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPSIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            2874..3866
                     /gene="proX"
                     /locus_tag="OQQ48_000103"
     CDS             2874..3866
                     /gene="proX"
                     /locus_tag="OQQ48_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HCS1951011.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTLLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKEPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            4158..5342
                     /locus_tag="OQQ48_000104"
     CDS             4158..5342
                     /locus_tag="OQQ48_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1951012.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            5466..6203
                     /gene="ygaZ"
                     /locus_tag="OQQ48_000105"
     CDS             5466..6203
                     /gene="ygaZ"
                     /locus_tag="OQQ48_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HCS1951013.1"
                     /translation="MESPTPQPALGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            6193..6528
                     /gene="ygaH"
                     /locus_tag="OQQ48_000106"
     CDS             6193..6528
                     /gene="ygaH"
                     /locus_tag="OQQ48_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HCS1951014.1"
                     /translation="MSYEVLLLGLLVGVANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            6619..7149
                     /gene="emrR"
                     /locus_tag="OQQ48_000107"
     CDS             6619..7149
                     /gene="emrR"
                     /locus_tag="OQQ48_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HCS1951015.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            7276..8448
                     /gene="emrA"
                     /locus_tag="OQQ48_000108"
     CDS             7276..8448
                     /gene="emrA"
                     /locus_tag="OQQ48_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HCS1951016.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            8465..10003
                     /gene="emrB"
                     /locus_tag="OQQ48_000109"
     CDS             8465..10003
                     /gene="emrB"
                     /locus_tag="OQQ48_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HCS1951017.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWECGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(10067..10582)
                     /gene="luxS"
                     /locus_tag="OQQ48_000110"
     CDS             complement(10067..10582)
                     /gene="luxS"
                     /locus_tag="OQQ48_000110"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCS1951018.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGSHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(10732..12288)
                     /gene="gshA"
                     /locus_tag="OQQ48_000111"
     CDS             complement(10732..12288)
                     /gene="gshA"
                     /locus_tag="OQQ48_000111"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCS1951019.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTQATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCCDISGTDAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLPQVGKDLFRDLKRVAQTLDSINGGKAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(12361..12789)
                     /locus_tag="OQQ48_000112"
     CDS             complement(12361..12789)
                     /locus_tag="OQQ48_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCS1951020.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISYPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(12786..13341)
                     /gene="yqaB"
                     /locus_tag="OQQ48_000113"
                     /pseudo
     CDS             complement(12786..13341)
                     /gene="yqaB"
                     /locus_tag="OQQ48_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
     gene            complement(13489..13565)
                     /locus_tag="OQQ48_000114"
     tRNA            complement(13489..13565)
                     /locus_tag="OQQ48_000114"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13529..13531),aa:Arg,seq:acg)
     gene            complement(13764..13840)
                     /locus_tag="OQQ48_000115"
     tRNA            complement(13764..13840)
                     /locus_tag="OQQ48_000115"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13804..13806),aa:Arg,seq:acg)
     gene            complement(14038..14114)
                     /locus_tag="OQQ48_000116"
     tRNA            complement(14038..14114)
                     /locus_tag="OQQ48_000116"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14078..14080),aa:Arg,seq:acg)
     gene            complement(14118..14210)
                     /locus_tag="OQQ48_000117"
     tRNA            complement(14118..14210)
                     /locus_tag="OQQ48_000117"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14174..14176),aa:Ser,seq:gct)
     gene            complement(14526..14711)
                     /gene="csrA"
                     /locus_tag="OQQ48_000118"
     CDS             complement(14526..14711)
                     /gene="csrA"
                     /locus_tag="OQQ48_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HCS1951021.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(14946..17576)
                     /gene="alaS"
                     /locus_tag="OQQ48_000119"
     CDS             complement(14946..17576)
                     /gene="alaS"
                     /locus_tag="OQQ48_000119"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCS1951022.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKDANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(17704..18204)
                     /gene="recX"
                     /locus_tag="OQQ48_000120"
     CDS             complement(17704..18204)
                     /gene="recX"
                     /locus_tag="OQQ48_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCS1951023.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYDEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(18447..19508)
                     /gene="recA"
                     /locus_tag="OQQ48_000121"
     CDS             complement(18447..19508)
                     /gene="recA"
                     /locus_tag="OQQ48_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCS1951024.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(19588..20085)
                     /gene="pncC"
                     /locus_tag="OQQ48_000122"
     CDS             complement(19588..20085)
                     /gene="pncC"
                     /locus_tag="OQQ48_000122"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HCS1951025.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMVIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(20230..21315)
                     /gene="mltB"
                     /locus_tag="OQQ48_000123"
     CDS             complement(20230..21315)
                     /gene="mltB"
                     /locus_tag="OQQ48_000123"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HCS1951026.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLATAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            21571..22134
                     /gene="srlA"
                     /locus_tag="OQQ48_000124"
                     /gene_synonym="gutA"
     CDS             21571..22134
                     /gene="srlA"
                     /locus_tag="OQQ48_000124"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HCS1951027.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSQGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            22131..23090
                     /gene="srlE"
                     /locus_tag="OQQ48_000125"
                     /gene_synonym="gutE"
     CDS             22131..23090
                     /gene="srlE"
                     /locus_tag="OQQ48_000125"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HCS1951028.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITTGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            23101..23472
                     /gene="srlB"
                     /locus_tag="OQQ48_000126"
                     /gene_synonym="gutB"
     CDS             23101..23472
                     /gene="srlB"
                     /locus_tag="OQQ48_000126"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCS1951029.1"
                     /translation="MTVIYKTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFTLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            23476..24255
                     /gene="srlD"
                     /locus_tag="OQQ48_000127"
     CDS             23476..24255
                     /gene="srlD"
                     /locus_tag="OQQ48_000127"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HCS1951030.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLVAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGESMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLVEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            24360..24718
                     /gene="gutM"
                     /locus_tag="OQQ48_000128"
                     /pseudo
     CDS             24360..24718
                     /gene="gutM"
                     /locus_tag="OQQ48_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
     gene            24785..25558
                     /gene="srlR"
                     /locus_tag="OQQ48_000129"
                     /gene_synonym="gutR"
     CDS             24785..25558
                     /gene="srlR"
                     /locus_tag="OQQ48_000129"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HCS1951031.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESEPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKLASFHGQLAENA
                     FEHFSFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            25551..26516
                     /gene="gutQ"
                     /locus_tag="OQQ48_000130"
     CDS             25551..26516
                     /gene="gutQ"
                     /locus_tag="OQQ48_000130"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HCS1951032.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(26513..28027)
                     /gene="norR"
                     /locus_tag="OQQ48_000131"
     CDS             complement(26513..28027)
                     /gene="norR"
                     /locus_tag="OQQ48_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HCS1951033.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKGVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMMPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGMKD"
     gene            28214..29653
                     /gene="norV"
                     /locus_tag="OQQ48_000132"
     CDS             28214..29653
                     /gene="norV"
                     /locus_tag="OQQ48_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HCS1951034.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNKVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFEELASEAK"
     gene            29650..30783
                     /gene="norW"
                     /locus_tag="OQQ48_000133"
                     /pseudo
     CDS             29650..30783
                     /gene="norW"
                     /locus_tag="OQQ48_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417191.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
     gene            complement(30911..33163)
                     /gene="hypF"
                     /locus_tag="OQQ48_000134"
     CDS             complement(30911..33163)
                     /gene="hypF"
                     /locus_tag="OQQ48_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HCS1951035.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLREDPETFLVQLHQHCPPLARIDSVEREPYIWSQLPTEFTIRQSAGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPKYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLAAFWQQWLNWQAQVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(33316..33843)
                     /gene="hydN"
                     /locus_tag="OQQ48_000135"
     CDS             complement(33316..33843)
                     /gene="hydN"
                     /locus_tag="OQQ48_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HCS1951036.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(33992..35002)
                     /gene="ascG"
                     /locus_tag="OQQ48_000136"
     CDS             complement(33992..35002)
                     /gene="ascG"
                     /locus_tag="OQQ48_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HCS1951037.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCNAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALAQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIHRDSLIAL
                     SR"
     gene            35262..36719
                     /gene="ascF"
                     /locus_tag="OQQ48_000137"
     CDS             35262..36719
                     /gene="ascF"
                     /locus_tag="OQQ48_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HCS1951038.1"
                     /translation="MAKNYAALARSEIAALGGVDNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTPRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASADIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYSIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAQARKHQSVQPTVAKEVSLN"
     gene            36728..38152
                     /gene="ascB"
                     /locus_tag="OQQ48_000138"
     CDS             36728..38152
                     /gene="ascB"
                     /locus_tag="OQQ48_000138"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HCS1951039.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     EITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LAAKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTIDKALGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(38311..38781)
                     /gene="hycI"
                     /locus_tag="OQQ48_000139"
     CDS             complement(38311..38781)
                     /gene="hycI"
                     /locus_tag="OQQ48_000139"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HCS1951040.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(38774..39184)
                     /gene="hycH"
                     /locus_tag="OQQ48_000140"
     CDS             complement(38774..39184)
                     /gene="hycH"
                     /locus_tag="OQQ48_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HCS1951041.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQQEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(39181..39948)
                     /gene="hycG"
                     /locus_tag="OQQ48_000141"
     CDS             complement(39181..39948)
                     /gene="hycG"
                     /locus_tag="OQQ48_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HCS1951042.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGELDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEVRIR"
     gene            complement(39948..40490)
                     /gene="hycF"
                     /locus_tag="OQQ48_000142"
     CDS             complement(39948..40490)
                     /gene="hycF"
                     /locus_tag="OQQ48_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HCS1951043.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(40500..42209)
                     /gene="hycE"
                     /locus_tag="OQQ48_000143"
     CDS             complement(40500..42209)
                     /gene="hycE"
                     /locus_tag="OQQ48_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HCS1951044.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(42227..43150)
                     /gene="hycD"
                     /locus_tag="OQQ48_000144"
     CDS             complement(42227..43150)
                     /gene="hycD"
                     /locus_tag="OQQ48_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HCS1951045.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(43153..44538)
                     /gene="hycC"
                     /locus_tag="OQQ48_000145"
                     /pseudo
     CDS             complement(43153..44538)
                     /gene="hycC"
                     /locus_tag="OQQ48_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
     gene            complement(44524..44784)
                     /locus_tag="OQQ48_000146"
                     /pseudo
     CDS             complement(44524..44784)
                     /locus_tag="OQQ48_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708526.1"
                     /note="regulates several genes involved in the formate
                     hydrogenlyase system; seems to prevent binding of FhlA
                     transcriptional activator to the activator sequence of hyc
                     operon; incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            44996..45346
                     /gene="hypA"
                     /locus_tag="OQQ48_000147"
     CDS             44996..45346
                     /gene="hypA"
                     /locus_tag="OQQ48_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCS1951046.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEVECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            45350..46222
                     /gene="hypB"
                     /locus_tag="OQQ48_000148"
     CDS             45350..46222
                     /gene="hypB"
                     /locus_tag="OQQ48_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HCS1951047.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            46213..46485
                     /gene="hypC"
                     /locus_tag="OQQ48_000149"
     CDS             46213..46485
                     /gene="hypC"
                     /locus_tag="OQQ48_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HCS1951048.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVITEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            46485..47606
                     /gene="hypD"
                     /locus_tag="OQQ48_000150"
     CDS             46485..47606
                     /gene="hypD"
                     /locus_tag="OQQ48_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HCS1951049.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            47603..48613
                     /gene="hypE"
                     /locus_tag="OQQ48_000151"
     CDS             47603..48613
                     /gene="hypE"
                     /locus_tag="OQQ48_000151"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HCS1951050.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPCYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            48687..50765
                     /gene="flhA"
                     /locus_tag="OQQ48_000152"
     CDS             48687..50765
                     /gene="flhA"
                     /locus_tag="OQQ48_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HCS1951051.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRRILSRPDTLHCSYEEFC
                     ETWPQLVAGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(50802..51155)
                     /locus_tag="OQQ48_000153"
     CDS             complement(50802..51155)
                     /locus_tag="OQQ48_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HCS1951052.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(51232..51366)
                     /locus_tag="OQQ48_000154"
     CDS             complement(51232..51366)
                     /locus_tag="OQQ48_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1951053.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIASVE
                     "
     gene            51442..54003
                     /gene="mutS"
                     /locus_tag="OQQ48_000155"
     CDS             51442..54003
                     /gene="mutS"
                     /locus_tag="OQQ48_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCS1951054.1"
                     /translation="MSAIENFDAHTPMMQQYLRLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAGLQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     DVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            54109..54765
                     /gene="pphB"
                     /locus_tag="OQQ48_000156"
     CDS             54109..54765
                     /gene="pphB"
                     /locus_tag="OQQ48_000156"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HCS1951055.1"
                     /translation="MPSTRYQKINAHHYRHIWVVGDIHGEYQLLQSRLHQLSFCPETD
                     LLISVGDNIDRGPESLDVLRLLNQPWFISVKGNHEAMELDAFATGDGNMWLASGGDWF
                     FDLNDSEQQEAIDLLLKFHHLPHIIEITNDIIKYVIAHADYPGDEYLFGKEIAESELL
                     WPVYRVQKSLNGELQQINGADYFIFGHMMFDNIQTFANQIYIDTGSPKSGRLSFYKIR
                     "
     gene            complement(54816..55583)
                     /locus_tag="OQQ48_000157"
     CDS             complement(54816..55583)
                     /locus_tag="OQQ48_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCS1951056.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            55779..56687
                     /locus_tag="OQQ48_000158"
     CDS             55779..56687
                     /locus_tag="OQQ48_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCS1951057.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGDAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            56684..57945
                     /locus_tag="OQQ48_000159"
                     /pseudo
     CDS             56684..57945
                     /locus_tag="OQQ48_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
     gene            57942..58579
                     /locus_tag="OQQ48_000160"
                     /pseudo
     CDS             57942..58579
                     /locus_tag="OQQ48_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
     gene            58584..59360
                     /locus_tag="OQQ48_000161"
     CDS             58584..59360
                     /locus_tag="OQQ48_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HCS1951058.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAMEFLFPYDYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFAPHGKRILVEALSPDVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDAWR"
     gene            59449..60813
                     /locus_tag="OQQ48_000162"
     CDS             59449..60813
                     /locus_tag="OQQ48_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HCS1951059.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVSFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVATAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINKRQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMTGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(60907..61899)
                     /gene="rpoS"
                     /locus_tag="OQQ48_000163"
     CDS             complement(60907..61899)
                     /gene="rpoS"
                     /locus_tag="OQQ48_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HCS1951060.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(61962..63053)
                     /gene="nlpD"
                     /locus_tag="OQQ48_000164"
     CDS             complement(61962..63053)
                     /gene="nlpD"
                     /locus_tag="OQQ48_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HCS1951061.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGSY
                     SGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITGG
                     NAITQADAAEQGVVIKTAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPAATTVTA
                     PVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQAI
                     IATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATMG
                     STGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(63193..63819)
                     /gene="pcm"
                     /locus_tag="OQQ48_000165"
     CDS             complement(63193..63819)
                     /gene="pcm"
                     /locus_tag="OQQ48_000165"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HCS1951062.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(63813..64574)
                     /gene="surE"
                     /locus_tag="OQQ48_000166"
     CDS             complement(63813..64574)
                     /gene="surE"
                     /locus_tag="OQQ48_000166"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HCS1951063.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(64555..65604)
                     /gene="truD"
                     /locus_tag="OQQ48_000167"
     CDS             complement(64555..65604)
                     /gene="truD"
                     /locus_tag="OQQ48_000167"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HCS1951064.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQHRVNDKELMITAALPGSGEWGTQRKALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(65601..66080)
                     /gene="ispF"
                     /locus_tag="OQQ48_000168"
     CDS             complement(65601..66080)
                     /gene="ispF"
                     /locus_tag="OQQ48_000168"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HCS1951065.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(66080..66790)
                     /gene="ispD"
                     /locus_tag="OQQ48_000169"
     CDS             complement(66080..66790)
                     /gene="ispD"
                     /locus_tag="OQQ48_000169"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HCS1951066.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(66809..67120)
                     /gene="ftsB"
                     /locus_tag="OQQ48_000170"
     CDS             complement(66809..67120)
                     /gene="ftsB"
                     /locus_tag="OQQ48_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HCS1951067.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(67314..67637)
                     /locus_tag="OQQ48_000171"
     CDS             complement(67314..67637)
                     /locus_tag="OQQ48_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HCS1951068.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(67687..68292)
                     /gene="cysC"
                     /locus_tag="OQQ48_000172"
     CDS             complement(67687..68292)
                     /gene="cysC"
                     /locus_tag="OQQ48_000172"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HCS1951069.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(68292..69719)
                     /gene="cysN"
                     /locus_tag="OQQ48_000173"
     CDS             complement(68292..69719)
                     /gene="cysN"
                     /locus_tag="OQQ48_000173"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HCS1951070.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTHIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMSWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKSLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     PSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYPVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPKNEAAVASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(69721..70629)
                     /gene="cysD"
                     /locus_tag="OQQ48_000174"
     CDS             complement(69721..70629)
                     /gene="cysD"
                     /locus_tag="OQQ48_000174"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HCS1951071.1"
                     /translation="MAQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            70881..71918
                     /gene="iap"
                     /locus_tag="OQQ48_000175"
     CDS             70881..71918
                     /gene="iap"
                     /locus_tag="OQQ48_000175"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HCS1951072.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            complement(72125..72418)
                     /gene="cas2"
                     /locus_tag="OQQ48_000176"
     CDS             complement(72125..72418)
                     /gene="cas2"
                     /locus_tag="OQQ48_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2"
                     /protein_id="HCS1951073.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNTESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
     gene            complement(72418..73338)
                     /gene="cas1e"
                     /locus_tag="OQQ48_000177"
     CDS             complement(72418..73338)
                     /gene="cas1e"
                     /locus_tag="OQQ48_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1"
                     /protein_id="HCS1951074.1"
                     /translation="MTFVPLSPIPLKDRTSMIFLQYGQIDVLDGAFVLIDKTGIRTHI
                     PVGSVACIMLEPGTRVSHAAVHLAATVGTLQVWVGEAGVRVYSSGQPGGARADKLLYQ
                     AKLALTEDLRLKVVRKMYELRFREPPPARRSVEQLRGIEGSRVRQTYALLAKQYGVKW
                     NGRKYDPKDWEKGDVVNRCISAATSCLYGISEAAVLAAGYAPAIGFIHSGKPLSFVYD
                     IADIIKFDSVVPKAFEIAARQPAEPDKEVRLACRDIFRSTKLTGKLIPLIEEVLAAGE
                     IEPPQPAPDMLPPAIPEPETLGDSGHRGRG"
     gene            complement(73335..73985)
                     /gene="cas6e"
                     /locus_tag="OQQ48_000178"
     CDS             complement(73335..73985)
                     /gene="cas6e"
                     /locus_tag="OQQ48_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HCS1951075.1"
                     /translation="MYLSRITLHTGQLSPAQLLHLVDRGEYVMHQWLWDLFPSGKERQ
                     FLYRREELQGAFRFFVLSQERPAESDTFTIECRSFAPELCTGQQLCFNLRANPTICKA
                     GKRHDLLMEAKRQVRGQAEGSDVWLHQQQAALDWLAAQGERSGFTLLDISVDAYRQQQ
                     LRRENSRQLIQFSSVDYTGMLTVTDPGLFLQRLSQGYGKSRAFGCGLMLIKPGAEA"
     gene            complement(73967..74713)
                     /gene="cas5e"
                     /locus_tag="OQQ48_000179"
     CDS             complement(73967..74713)
                     /gene="cas5e"
                     /locus_tag="OQQ48_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HCS1951076.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSLSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSDPE
                     LLSAIISRRDYYTDAWWMVAVATTADAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNACDALCNAYQQYQDHFHKLKVSLPKLQDECWWEGKHDGLVASKILRRRDV
                     PLNRQQWLFGERTVNQGPWLSKEEPCTSQE"
     gene            complement(74724..75802)
                     /gene="cas7e"
                     /locus_tag="OQQ48_000180"
                     /pseudo
     CDS             complement(74724..75802)
                     /gene="cas7e"
                     /locus_tag="OQQ48_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
     gene            complement(75817..76385)
                     /gene="casB"
                     /locus_tag="OQQ48_000181"
                     /pseudo
     CDS             complement(75817..76385)
                     /gene="casB"
                     /locus_tag="OQQ48_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
     gene            complement(76382..77944)
                     /gene="casA"
                     /locus_tag="OQQ48_000182"
     CDS             complement(76382..77944)
                     /gene="casA"
                     /locus_tag="OQQ48_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HCS1951077.1"
                     /translation="MNSFSLLTTPWLPVRFKDGTTGKLAPVDLADENVVDIAAPRADL
                     QGAAWQFLLGLLQTSFAPKDQRSWDDIWEDGLEAEKLREALLSLEHAFQFGPDSPSFM
                     QDFDELKVKATFIASLLPDAPGKQTKERNTDHFIKRDTTQHLCLHCVPLALFSIQLNA
                     PIGGRGYYPGLRGGGPLTTLIELLEYQGNQQTPLWRKLWLNVMPQDEADLPLPKTFDD
                     LVFPWLAPTRTSELDGAVVTDEQVNKLQAYWGMPRRIRIDFKTTSIGNCDICGRQSDA
                     LLGLMSLKNYGVQYVMWRHPLTPYRLPLKEGGDFYSVKPQPGGLIWRDWLGLIEVGNS
                     KNNTELPAQVVKLLNASNLKQTRVGLWGFGFDFEDMKVRCWYEHHFPLLLNKKEDLIP
                     KLRLAAQAASHILSLLHRALKEAWFSEKKTTKLDFGFVDIDFWNKTQHRFLRLVRKIE
                     EGQDPDELLSKWQKEMWLFARQDFDDRVFTNPYEPVDLERVMTARKKYFTTSAEKQNA
                     NAAREKKQEAAE"
     gene            complement(78042..79594)
                     /gene="cas3"
                     /locus_tag="OQQ48_000183"
                     /pseudo
     CDS             complement(78042..79594)
                     /gene="cas3"
                     /locus_tag="OQQ48_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142534.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
BASE COUNT        18775 a        21585 c        20336 g        18898 t
ORIGIN      
        1 ctatactttt gcgggtcaac ataagaggat gagtccttaa taaaacaggt cctcagagca
       61 tacatattct gcggagcgtg cggcaacaac aggacgaaca ggggcggttt tcaatttgca
      121 gccgtcaggc tgccgtggtt caggctcctc ttatgtgatg gggaaagcgg ttgaaacaga
      181 agatgaagac tggaatttct gagggtgtta ttttcaaaaa tatcactacc cgcagcaggg
      241 aaataattcc cgccaaatag ctttttatca cgcaaatatt ctgtagtgat ctacactgag
      301 actctgttgc attattcgcc tgaaactaca atattcaggc gttttttcgc tatctatgac
      361 aaaaaatatc aacttttctc gatttgctct cagcccttat atcacgggaa attccggcga
      421 tttgctcgca tcaatattca tgccacattt gccctcaggg gttgcctcag attctcagta
      481 tgttagggta gaaaaaagtg actatttcca ttgggtaata tatcgacata aacaaataaa
      541 ggaatctttc tattgcatgg caattaaatt agaaattaaa aatctttata aaatatttgg
      601 cgagcatcca cagcgagcgt tcaaatatat cgaacaagga ctttcgaaag aacaaattct
      661 ggaaaaaact gggctatcgc ttggcgtaaa agacgccagt ctggccattg aagaaggcga
      721 gatatttgtc atcatgggat tatccggctc gggtaaatcc acaatggtac gccttctcaa
      781 tcgcctgatt gaacccaccc gcgggcaagt gctgattgat ggtgtggata ttgccaaaat
      841 atccgatgcc gaactccgtg aggtgcgcag aaaaaagatt gcgatggtct tccagtcctt
      901 tgccttaatg ccgcatatga ccgtgctgga caatactgcg ttcggtatgg aattggccgg
      961 aattaatgcc gaagaacgcc gggaaaaagc ccttgatgca ctgcgtcagg tcgggctgga
     1021 aaattatgcc cacagctacc cggatgaact ctctggcggg atgcgtcaac gtgtgggatt
     1081 agcccgcgcg ttagcgatta atccggatat attattaatg gacgaagcct tctcggcgct
     1141 cgatccatta attcgcaccg agatgcagga tgagctggta aaattacagg cgaaacatca
     1201 gcgcaccatt gtctttattt cccacgatct cgatgaagct atgcgtattg gcgaccgaat
     1261 tgccattatg caaaatggtg aagtggtaca ggtcggcaca ccggatgaaa ttctcaataa
     1321 tccggcgaat gattatgtcc gtaccttctt ccgtggcgtt gatattagtc aggtattcag
     1381 tgcgaaagat attgcccgcc ggacaccgaa tggcttaatt cgtaaaaccc ctggcttcgg
     1441 cccacgttcg gcactgaaat tattgcagga tgaagatcgc gaatatggct acgttatcga
     1501 acgcggtaat aagtttgtcg gcgcagtttc catcgattcg cttaaaaccg cgttaacgca
     1561 gcagcaaggt cttgatgcgg cgctgattga tgcgccgtta gcagtcgatg cacaaacgcc
     1621 tcttagcgag ttgctctctc atgtcggaca ggcaccctgt gcggtacccg tggtcgacga
     1681 ggaccaacag tatgtcggca tcatttcgaa aggaatgctg ctgcgcgctt tagatcgtga
     1741 gggggtaaat aatggctgat caaaataatc cgtgggatac cacgccagcg gcggacagtg
     1801 ccgtgcaatc cgcagacgcc tggggtacac cggcgactgc accgactgac ggcggtggtg
     1861 ctgactggct gaccagtacg cctgcgccaa acgtcgagca ttttaatatt ctcgatccgt
     1921 tccataaaac gctgatcccg ctcgacagtt gggtcactga agggatcgac tgggttgtta
     1981 cccatttccg tcccgtcttc cagggcgtgc gcgttccggt tgattatatc ctcaacggtt
     2041 tccagcaatt gctgctgggt atgcccgcac cggtggcgat tatcgttttc gctctcatcg
     2101 cctggcagat ttccggggtc ggaatgggcg tggcgacgct ggtttcgctg attgccatcg
     2161 gcgcaatcgg tgcctggtcg caggccatgg ttaccctggc gctggtgtta accgccctgc
     2221 tgttctgtat cgtcattggt ttgccgctgg ggatctggct ggcgagaagt ccgcgagcgg
     2281 cgaaaattat tcgtccactg cttgatgcca tgcagaccac gccagcgttt gtttatctgg
     2341 tgccaatcgt catgctattt ggtatcggta acgtgccggg cgtggtggtg acgatcatct
     2401 ttgctctgcc gccgattatc cgtctgacca ttctggggat taaccaggtt ccggcggatc
     2461 tgattgaagc ctcgcgctca ttcggtgcca gcccgcgcca gatgctgttc aaagttcagt
     2521 taccgctggc gatgccgagc attatggcgg gcgttaacca gacgctgatg ctggcccttt
     2581 ctatggtggt cattgcctcg atgattgccg tcggcgggct gggtcagatg gtacttcgcg
     2641 gtatcggtcg tctggatatg gggcttgcca ccgttggcgg cgtcgggatt gtgatcctcg
     2701 ccattatcct cgatcgcctg acgcaggccg ttgggcgcga ctcacgcagt cgcggcaacc
     2761 gtcgctggta caccactggc cctgttggtc tgctgacccg cccattcatt aagtaactct
     2821 gcacttgccc ggtgacgccg ggcattatca ccctgccaaa aaaaggaata acaatgcgac
     2881 atagcgtact ttttgcgaca gcgtttgcca cgcttatctc tacacaaact tttgctgccg
     2941 atctgccggg caaaggtatt actgttaatc cagttcaaag caccatcacc gaagaaacct
     3001 tccagacgct gctggtcagc cgtgcgctgg agaaattagg ctataccgtc aacaaaccca
     3061 gcgaagtaga ttacaacgtt ggctacacct tgcttgcttc cggcgatgca accttcaccg
     3121 ccgtgaactg gacgccactg catgacaaca tgtacgaagc tgccggtggc gataagaaat
     3181 tttatcgtga aggggtattt gttaacggcg cggcacaggg ttacctgatc gataagaaaa
     3241 ccgccgacca gtacaaaatc accaacatcg cacaactgaa agagccgaag atcgccaaac
     3301 tgttcgatac caacggcgac ggtaaagcgg atttaaccgg ttgtaatcct ggctggggct
     3361 gcgaaggtgc gatcaaccac cagcttgccg cgtatgaact gaccaacacc gtgacgcata
     3421 atcaggggaa ctacgcagcg atgatggccg acaccatcag tcgctacaaa gagggcaaac
     3481 cggtgtttta ttacacctgg acgccgtact gggtgagtaa tgagctgaag ccagggaaag
     3541 atgtggtctg gttgcaggtg ccgttctccg cactgccggg cgataaaaat gccgatacca
     3601 aactgccgaa tggcgcgaat tacggcttcc cggtcagcac catgcatatc gttgccaaca
     3661 aagcctggac cgagaaaaat ccggcggcag ccaaactgtt tgccattatg cagttgccag
     3721 tggccgatat taacgcccag aacgccatta tgcatgacgg caaagcctca gaaggcgata
     3781 ttcagggcca cgttgatggc tggatcaaag cccaccagca gcagttcgat ggctgggtga
     3841 atgaggcgct ggcagcgcag aagtaatttt tattcaggcg gataaggcgt ttttgcctta
     3901 tccgccgctc tgtacacaat gcctgatgcg acgctgacgc gtcttatcat gcctacaaaa
     3961 tacactcatt ccgtatggcg gataaggcgt tttcgccgca tccgccgttc tgtgcacaat
     4021 gcctgatgcg acgctggcgc gtcttatcat gcctacaaac cacatcgcac atttcaacaa
     4081 gctatctttc atcccatatt catcaacatc cgctattatt gatttccagc ttaatcatca
     4141 cctgatgaac aaaaataatg actaaaccta atcatgagct tagcccggcg ctgatcgtgc
     4201 tgatgtctat cgccaccggt ctggcggtag ccagtaacta ttacgcccag ccattgctcg
     4261 acaccatcgc gcgtaacttt tccctttccg ccagttcggc aggctttatt gttaccgccg
     4321 cgcaattggg ctatgcagca ggtctgctgt ttcttgttcc cctcggtgat atgtttgaac
     4381 gccgccgcct gattgtctcg atgaccttac tggcggcagg cggcatgtta atcactgcca
     4441 gcagtcagtc gctggcgatg atgatcctcg gtacggcatt aaccggttta ttctctgtcg
     4501 tggcacaaat tctggttccg ctggcagcga cgctggcttc accggacaaa cgcggcaaag
     4561 tggttggcac tattatgagc ggtctgctgt tggggatctt actcgcacgg accgttgccg
     4621 gattgctggc gaatctcggc ggctggcgca ccgtcttttg ggttgcctcg gtgttaatgg
     4681 cactgatggc gctggcatta tggcgtggtc tgccacaaat gaaatcagaa acccacctca
     4741 actacccaca gttgttaggt tccgttttca gcatgtttat cagcgataag atcctgcgca
     4801 cccgcgcatt gctgggctgc ctgacctttg ccaacttcag cattctctgg acctcaatgg
     4861 cctttttgct tgccgctcca ccttttaact acagcgatgg tgtcattggt ctgtttggac
     4921 ttgcgggagc tgccggagcg ttgggcgctc gtccggcggg cggttttgcc gataagggta
     4981 aatcgcacca caccacaact ttcggtctgc tgctgctatt actttcatgg ctggcgatct
     5041 ggtttggtca cacttccgta ctggcgctga ttatcggcat cctggtgctg gatctcaccg
     5101 tacagggcgt gcatatcact aaccagacgg taatttatcg aatacaccct gatgcgcgta
     5161 atcgcctgac cgcaggttac atgaccagct actttattgg cggtgccgcc ggttcgctaa
     5221 tttcagcctc agcctggcaa catggcggtt gggctggcgt ttgtctggct ggcgcgacga
     5281 ttgccctggt taacttactg gtctggtggc gaggttttca tcgtcaggaa gccgcaaatt
     5341 aagcaatcag caaccgtttt cgtggcttta cacttataag ggtgttaaga agcccatcag
     5401 tctgataagg ttaagatatt cattcagtct atttataata ttaacaatcg ttaagcgtac
     5461 actctatgga aagccctact ccacagcctg ctcttggttc ggcgaccttc atggaaggat
     5521 gcaaagacag tttaccgatt gttattagtt atattccggt ggcctttgcg ttcggtctga
     5581 atgcgacccg tctgggattc tctcctctcg aaagcgtttt tttctcctgc atcatttatg
     5641 caggcgcgag ccagttcgtc attaccgcga tgctggcagc cgggagtagt ttgtgggttg
     5701 ctgcactgac cgtcatggca atggatgttc gccatgtgtt gtatggcccg tcactgcgta
     5761 gccgtattat tcagcgtctg caaaaatcga aaaccgccct gtgggcattt ggcctgacgg
     5821 atgaggtttt tgccgccgca accgcaaaac tggtacgcaa taatcgccgc tggagcgaga
     5881 actggatgat cggcattgcc ttcagttcat ggtcatcgtg ggtatttggt acggtaatag
     5941 gggcattctc cggcagcggc ttgctgcaag gttatcccgc cgttgaagct gcattaggtt
     6001 ttatgcttcc ggcactcttt atgagtttcc tgctcgcctc tttccagcgc aaacaatctc
     6061 tttgcgttac cgcagcgtta gttggtgccc ttgcaggcgt aacgctattt tctattcccg
     6121 tcgccattct ggcaggcatt gtctgtggct gcctcactgc gttaatccag gcattctggc
     6181 aaggagcgcc cgatgagcta tgaggttctg ctgcttgggt tactggttgg cgtggcgaat
     6241 tattgcttcc gctatttgcc gctgcgcctg cgtgtgggta atgcccgccc aaccaaacgt
     6301 ggcgcggtag gtattttgct cgacaccatt ggcatcgcct cgatatgcgc tctgctggtt
     6361 gtctctaccg caccagaagt gatgcacgat acacgccgtt tcgtgcccac gctggtcggc
     6421 ttcgcggtac tgggtgccag tttctataaa acacgcagca ttatcatccc aacactgctt
     6481 agtgcgctgg cctatgggct cgcctggaaa gtgatggcga ttatataacc cacaagaatc
     6541 atttttctaa aacaatacat ttactttatt tgttactgtc gttactatat cggctgaaat
     6601 taatgaggtc atacccaaat ggatagttcg tttacgccca ttgaacaaat gctaaaattt
     6661 cgcgccagcc gccacgaaga ttttccttat caggagatcc ttctgactcg tctttgcatg
     6721 cacatgcaaa gcaagctgct ggagaaccgc aataaaatgc tgaaggctca ggggattaac
     6781 gagacgttgt ttatggcgtt gattacgctg gagtctcagg aaaaccacag tattcagcct
     6841 tctgaattaa gttgtgctct tggatcatcc cgtaccaacg cgacgcgtat tgccgatgaa
     6901 ctggaaaaac gcggttggat cgaacgtcgt gaaagcgata acgatcgccg ctgcctgcat
     6961 ctgcaattaa cggaaaaagg tcacgagttt ttgcgcgagg ttttaccacc gcagcataac
     7021 tgcctgcatc aactctggtc cgcgctcagc acaacagaaa aagatcagct cgagcaaatc
     7081 acccgcaaat tgctctcccg tctcgaccag atggaacaag acggtgtggt tctcgaagcg
     7141 atgagctaac gcgtcatctc gctcaaaaat ccagatttat aaaagaaaaa atgaccggcc
     7201 agcatcgcaa catgctggcc tttttggcaa gcaggtcggc tcagccgatg agttaagaag
     7261 atcgtggaga acaatatgag cgcaaatgcg gagactcaaa ccccgcagca accggtaaag
     7321 aagagcggca aacgtaagcg tctgctcctc cttctcacct tgctctttat aattattgcc
     7381 gtagcgatag ggatttattg gtttttggta ctgcgtcatt tcgaagaaac cgatgacgca
     7441 tacgtggcag ggaatcagat tcaaattatg tctcaggtgt ctggcagcgt gacgaaagtc
     7501 tgggccgata acaccgattt tgtaaaagaa ggcgatgttc tggtcactct cgacccgaca
     7561 gatgctcgcc aggcgtttga aaaagccaaa actgcactgg cttccagcgt tcgccaaacc
     7621 caccagctga tgattaacag caaacagtta caggcgaata ttgaggtgca aaaaatcgcc
     7681 ctcgcgaaag cacaaagcga ctacaaccgc cgtgtgccgc tgggcaatgc caacctgatt
     7741 ggtcgcgaag agctgcaaca cgcccgcgac gccgtcacca gtgcccaggc gcaactggac
     7801 gtcgctattc aacaatacaa tgccaatcag gcgatgattc tggggactaa actggaagat
     7861 cagcctgccg tgcaacaggc tgccaccgaa gtacgtaacg cttggctggc gctggagcgt
     7921 acccgtattg tcagtccgat gaccggttat gtctcccgcc gcgcggtaca gcctggggcg
     7981 caaattagcc caacgacgcc gctgatggcg gtcgttccag ccaccaatat gtgggtggat
     8041 gccaacttta aagagacgca gattgccaat atgcgtatcg gtcagccggt cactatcacc
     8101 acggatattt acggcgatga tgtgaaatac accggtaaag tggttggtct ggatatgggc
     8161 acaggtagcg cattctcact gcttccggcg caaaatgcga ccggtaactg gatcaaagtc
     8221 gttcagcgtc tacctgtgcg tatcgaactg gaccagaaac agctggagca atacccgctg
     8281 cgcattggtt tgtccacgct ggtgagcgtc aataccacta accgtgacgg tcaggtactg
     8341 gcaaataaag tacgttcaac accggtcgcg gtaagtaccg cgcgtgaaat cagcctggca
     8401 cctgtcaata aactgatcga cgatatcgta aaagctaacg ctggctaatc cagaggtgcg
     8461 tgtgatgcaa cagcaaaaac cgctggaagg cgcgcaactg gtcattatga cgattgcgct
     8521 gtcactggcg acattcatgc aggtgctgga ctccaccatt gctaacgtgg cgatccccac
     8581 tatcgccggg aatctgggct catcgctcag ccagggaacg tgggtaatca cttctttcgg
     8641 ggtggcgaat gccatctcga tcccgcttac cggctggctg gcaaagcgcg tcggggaagt
     8701 gaaactgttc ctttggtcca ccatcgcctt tgctattgcg tcgtgggagt gtggtgtctc
     8761 cagcagcctg aatatgctga tcttcttccg tgtgattcag gggattgtcg ctgggccgtt
     8821 gatcccactt tcgcaaagtc ttttgctgaa taattacccg ccagccaaac gctcaatcgc
     8881 gctggcgttg tggtcgatga cggtgattgt cgcgccaatt tgcggcccaa tcctcggcgg
     8941 ttatatcagc gataattacc actggggctg gatattcttc atcaacgtgc caattggcgt
     9001 ggcggtggtg ttgatgacac tgcaaaccct gcgcggacgc gaaacccgca ccgaacggcg
     9061 gcggattgat gccgtggggc tggcactgct ggttattggt atcggtagcc tgcagattat
     9121 gctcgaccgc ggtaaagagc tggactggtt ttcatcacag gaaattatca ttcttaccgt
     9181 ggtggcggtg gtggctatct gcttcctgat tgtctgggag ctgaccgacg ataacccgat
     9241 agtcgatctg tcgttgttta agtcgcgcaa cttcaccatc ggctgcttgt gtatcagcct
     9301 cgcgtatatg ctctacttcg gcgcgattgt tctgctgccg cagttgttgc aggaggtcta
     9361 cggttacacg gcgacctggg caggtttggc ctctgcgccg gtagggatta ttccggtgat
     9421 cctgtcgccg attatcggcc gcttcgcgca taaactggat atgcggcggc tggtaacctt
     9481 cagctttatt atgtatgccg tctgcttcta ctggcgtgcc tatacctttg aaccaggtat
     9541 ggattttggc gcgtcggcct ggccgcagtt tatccagggg tttgcggtgg cctgcttctt
     9601 tatgccgctg accaccatta cgctgtctgg tttgccaccg gaacgactgg cggcggcatc
     9661 gagcctctct aactttacgc gaacgctggc ggggtctatc ggcacgtcga taaccacgac
     9721 catgtggacc aaccgcgagt cgatgcacca tgcgcagttg actgagtcgg taaacccgtt
     9781 caacccgaat gcccaggcga tgtacagtca actggaaggg cttgggatga cgcaacagca
     9841 ggcatcaggc tggattgccc agcagatcac taatcagggg ctgattattt ccgccaatga
     9901 gatcttctgg atgtcagccg ggatattcct cgtcctgctg gggctggtgt ggtttgctaa
     9961 accgcccttt ggcgcaggtg gcggcggagg tggtgcgcac taagtacaac taagccagtt
    10021 catttgaact ggcttttttc aattaattgt gaagatagtt tactgactaa atgtgcagtt
    10081 cctgcaactt ctctttcggc agtgccagtt cttcgttgct gttgatgcgt acgtcacgtt
    10141 ccagaatgct acgcgcaata tcctgcgctt cctgcaacga gtgcatctgg taagtgccac
    10201 actggtagac gttcagctcc gggatctgat tctgatcctg cactttcagc acgtcttcca
    10261 ttgccgcttt ccaggcatca gcaacacgct gctcatctgg cgtaccaatc agactcatat
    10321 aaaaaccggt gcggcagccc attggcgaga tatcgataat ctctacacca ttaccgttaa
    10381 gatggttacg cataaaacca gcaaacaggt gctccagagt atggctccct ctttctggca
    10441 tcacttcttt gttcggcacg cagaagcgca gatcgaacac ggtgattgcg tcgccatgcg
    10501 gggtgttcat tgttttcgcc acccgaactg caggcgcttc catccgggta tgatcgactg
    10561 tgaagctatc taacaacggc atttagccac ctccggtaat ttttttaaaa attttctgaa
    10621 ctctttcttc ccaggcgagt ctgagtatat gaaagacgcg catttgttat catcatccct
    10681 gaattcagag atgaaatttt ggccactcac gagtggcctt tttcttttct gtcaggcgtg
    10741 tttttccagc cacaccgcaa acggttcggt atcagcggtt tccatttcct gctgacggcg
    10801 ttcagatgcc tcgcgctcgg ctacaaaatc ctcttcgcgc agaatttcca acggctcttc
    10861 acgcagcaga ttacggtagg cttctgcaaa cgctttgcct gttccgccaa taccagtatc
    10921 aatcatagac cttaagatac gggcagagaa agtcagatcg ggattatcga agcaggcgac
    10981 cagttcatca cacactttct gatacgcttt gccgccgtta atgctatcca acgtttgcgc
    11041 gacgcgtttc agatcgcgga acagatcttt acccacctgc ggtaacggga actgcgcggt
    11101 ttcgcagccg atacccagcg tcaggcccgg tttgcgacct tcgaggatca cccggttcca
    11161 gttaacgcgt gtacaggcaa gttcgctact gctcatttcc ggtgcatcag ccagcgcaca
    11221 ccagaccata aacaggtcga ggaatcgcac ctgctgttca tctacaccaa tcggcgagaa
    11281 cgggttgatg tccagcgaac gcacttcaat atattcaatg ccgccacgta acagcgcatc
    11341 agaaggtgac tcgccgctgc gggtaacgcg ttttggacga atcggcgcat acagttcgtt
    11401 ttcaatctgc agcacgttgc tattgatttg cagcctctta ccgtctttct caataccaat
    11461 cttcgcgtac tcttccgatg gcgttttgat tgcctgttta aggcccgcta cgtactcgta
    11521 aagatcgttg aaggtaatac caagattgct ttgcgattta ttggtatagc cgagatcgct
    11581 caaacgaaga gaggtcgcat acggcaggta atacataccg cactcggttt tctcaaacgg
    11641 cagcgacgtt ggttttcctt gcaggaaaga agaacaaatc gccggagatg caccaaacag
    11701 ataaggaatg acccaaccga aacgatagta attgcggata acgcggaaat agcccgcaga
    11761 aattttctct ttggcatcag tgcccgagat atcacagcac ttcgcttgcc agaatgccat
    11821 tggcaaagag aaattgtagt gcacgccgga aatggtttgc atcagcgcgc cgtagcgatt
    11881 tttcagccct tcacgataca gcgttttaaa gcgtccggtg ttagaagtgc cgtactgtgc
    11941 cagttcgatg tcctgacctt ctgcgatgta gcatggcata cttaacggcc acatccgctc
    12001 atcgcccata ttgcgcgccg tataacgatg cagatcgcgc ataaaggtca acatatgttc
    12061 aatatcacca tccactggtg taatgaattc cagcaatgct tccgcaaaat cggtagtaat
    12121 ccatttgtgc gtcagtgcgg aacctaatgc ttcaggatga cctgttgttg cctgtgtgcc
    12181 atcagcatta acacgcaaag tttcgcgctc cagcccacgc tgtatcccct ttaacgcctg
    12241 aggatgtttt tccagccagg ccagcgcctg tgatacgtcc gggatcaaat tgacctcccg
    12301 cctgtcaaaa tcgttttaat tagcataact gtaatggtga ccatatgtgc aggcctacaa
    12361 ttagtgccac cacatcatgc cctgaacggt agctgctgca actgcaacat agcgtaaagc
    12421 tttaccaagg cacaaaaaaa agattaccgg tccccacgag atgcgcatcc atcccgctaa
    12481 cagacacagt aaatcgccaa ccacgggcat ccagcttaat aatagcgtga ctgcaccata
    12541 gcgtttcagc cagccagtag ctttctcttg ccagcgcgat gttttacgca atggaaagaa
    12601 acgcccaagg ataacgttag ttaaccctcc aaggctatta cccattgttg ctgttaaaac
    12661 taaaacccag ggataactga tcccggaaag caacattgcc accagcacga cttcggagtt
    12721 gccgggtaat agtgtagcgc tgagaaaact actggcaaac aacgagaaaa gcgataacgc
    12781 ttcactcaca gcaagcgaac atccacggcg tccatgcctg ctgcacgggc cgcctgaata
    12841 ccgaaatcgg catcttcaaa gaccacacac tgcgtcggtt gcacgcccat acgctgcgcg
    12901 cacaacaaaa atgtgtctgg cgcgggttta tggtgtttga cgtgatcagc agcgacgacg
    12961 gcgtcaaaat aacggcgtaa tcccaggtgc gccagcaatg cctcagcgat ggcgctttca
    13021 ctccccgttc ctacagccat tgggcgacga ccatgccaac ttttcaccac ttcaacaaga
    13081 ggaagcggct cgacactatc cagcagcata cttcttactg cttctgtttt ttcacgcgct
    13141 aacgcatgcg gggcgagatc ggcctgattc agctcaataa ttgcctgagc aatacgccag
    13201 gtgggcgatc cattaagcgc aatcattgcc tgaacatcgt actgaagacc gtagtgccct
    13261 aatacttcgc gccacgcttt acggtgcgta ggctccgtat ccaggaatat caaaaattaa
    13321 acctgcataa cgctcgtaca tggtcttctc gcaagtcgaa aaatatgacg ctactttagc
    13381 gtaattgctt gattttgtcg ctgatggcga aggagaagaa aattcaggaa atggataaag
    13441 tagttattta gaaatgagat atttttgaag gaaatttttg ggagaagatg gtgcatccgg
    13501 gaggattcga acctccgacc gctcggttcg tagccgagta ctctatccag ctgagctacg
    13561 gatgcatcgg gaaaatttat tttactgctg atattgatat cgctactaaa gccatatcaa
    13621 gtaagagatg gtgcatccgg gaggattact cgccttgcgg ctcgcccttc gggccgttgc
    13681 taaagcaacg ttatcatccc tggtgcctgc ggttaattcc gctacctctt cgataccttc
    13741 attgctgaag atttcggaga atatggtgca tccgggagga ttcgaacctc cgaccgctcg
    13801 gttcgtagcc gagtactcta tccagctgag ctacggatgc atcgggaaac ttactttact
    13861 gcagattttt tgataccgct actaaagccg tatcaagtaa gagatggtgc attcgggagg
    13921 attactcgcc ttgcggctcg cccttctggc cgttgctaaa gcaacgttat cctccctggt
    13981 gcctgcggtt ataccgccac ctcttccata ccttcatcgc tgaaagtacg tagaatatgg
    14041 tgcatccggg aggattcgaa cctccgaccg ctcggttcgt agccgagtac tctatccagc
    14101 tgagctacgg atgcaaatgg cggtgaggcg gggattcgaa ccccggatgc agcttttgac
    14161 cgcatactcc cttagcaggg gagcgccttc agcctctcgg ccacctcacc acacgcctct
    14221 tacgagtgct tcgaagaact tgtttatcgc tcatcgtcgc tgcgtggcgc acatattact
    14281 ttctgagact tataagtcaa acaatttttc ccacactttt atcgtttgca cacttcacgc
    14341 tcaattagtc tgtaaaaacg tcaaaaagag tgttttatct acagaaaaac ggaggtctgg
    14401 cagatagcag taatgcaaaa aaatggagac ttaagttgaa tgaacgggag taaagcgaaa
    14461 agactataga gtgaaggaga aattttgagg gtgcgtctca ccgataaaga tgagacgcgg
    14521 aaagattagt aactggactg ctgggatttt tcagcctgga tacgctggta gatctcttca
    14581 cggtgaacag aaacttcctt cggggcattt acgccaatac gtacctggtt gcccttcacc
    14641 cctaaaactg tcacggtgac ctcatcccca atcatgaggg tctcaccaac tcgacgagtc
    14701 agaatcagca ttctttgctc cttgaaagat taaaagagtc gggtctctct gtatcccggc
    14761 attatccatc atataacgcc aaaaagtaag caatgacaaa cacattacat gtaagcagtc
    14821 atggcattac attctgttaa acctaagttt agccgatata cacaacttca acctgacttt
    14881 atcgttgtcg atagcgttaa tgcgaatgcc gtgaagcgag tccacggcat tgcctgacgc
    14941 ttatattatt gcaatttcgc gctgacccag cctttcacac tggctaacgc tgcaggtaag
    15001 gccgcagcat ccgtaccacc ggcttgcgcc atgtcaggac gtccaccacc cttgccgccc
    15061 acctgctgag cgaccatacc aatcagttcc cctgctttca cacgatctgt gacgtcctta
    15121 gatacgcctg caatcagaga aaccttacct tcggctaccg ttgccagcac gataattgtc
    15181 gaccccagct gattttttaa atcgtcaacc atggtacgca acattttcgg ctcaacaccg
    15241 ctaagctcgc taaccaacag cttaacacca ttaacatcaa ttgccttact ggaaagattt
    15301 gcgctctcct gtgcggcagc ttgttcttta agctgttgta gttctttttc taactgacgc
    15361 gtacgttcca gtactgagcg cactttatcc gccagattat tgctatcgcc tttcagcaga
    15421 tgcgcgactt cgcttaagcg atcgctgtct gcatgaacgg tggtgatagc gccttctccg
    15481 gttaccgctt cgatacgacg aacgcctgca gcagtacccg attcagagat gatgcggaac
    15541 agaccaatat caccagtgcg gctggcgtga gtaccgccac acagctcggt ggagaaatcg
    15601 cccatgctca gcacgcgtac acgctcatca tacttctcgc cgaacagcgc catcgcacct
    15661 ttcgctttcg ccgcttcgag atccatgatg ttggtttcga tcggcaaatt gcgacgaatc
    15721 tgtgcgttca ccaggtcttc gaccgcacga atctcttccg gtttcatcgc ttcgttgtgt
    15781 gagaagtcga agcgcagcac cttgtcgtta accagtgaac ctttctgcga tacatgagtc
    15841 cccagaacct ggcgcagcgc agcgtgcatc aggtgcgttg cggagtgatt cagacgaata
    15901 cgggcgcgac gagcctcatc aacatcagcc tgcacagcgt cgcccacttt cagagaaccc
    15961 gcagcaagtt taccgatgtg accaatcgcc tggccgtatt tctgcgtatc ttccaccgcg
    16021 aaggagaagt tagcgtcttt cagttcgcct ttatcaccaa cctgaccgcc ggattccgca
    16081 tagaatggtg tttgatctag cacgaccaca gcttcctggc ctgcattgat ggcatcaacc
    16141 gctttaccat caacaaacag cgcggtcact ttgccgttca gttccagatg gtcatagcct
    16201 ttaaattcag atgcactatc aacacggatc attgcgttgt aatcggcacc aaagccgctg
    16261 gcttcgcgcg cgcgacgacg ctgctcttcc attgctgctt caaaaccagc ttcgtcaact
    16321 ttgatgttgc gctcacgaca aacatcagcc gtcaggtcaa ccgggaagcc ataggtgtcg
    16381 tacagacgga aagccgtttc accatccagc gtatcaccag aaagttttgc cagctcttca
    16441 tccagcaacg ccagaccgcg ctccagagta cgggcaaact gctcttcttc agtcttcagc
    16501 acctgctcaa cctgcgcctg ctggcgtttc aggtcttcac ccgcagagcc cataacgtcg
    16561 atcagcggac caaccagttt gtagaagaag gtttctttcg cgccgagcat attgccgtga
    16621 cgcactgcgc gacgaatgat acgacgcagt acataaccac ggttttcatt ggacggcatt
    16681 acgccatccg cgatcaggaa cgcacaagaa cgaatgtggt cagcgattac gcgcagcgat
    16741 ttattgctca gatcggtcgc gccagtgact ttcgctaccg cctggatcaa cgtgcggaac
    16801 aggtcgatgt catagttaga gttaacgtgt tgcagcaccg cagcaatacg ctccagaccc
    16861 ataccggtat ctacagacgg cttcggcagc ggttccatcg tgccatctgc ctggcggttg
    16921 aactgcatga agacgatgtt ccagatctca atgtagcggt cgccgtcttc ttccggactt
    16981 cccggagggc caccccaaat gtggtcgccg tgatcgtaga agatttcggt gcacgggccg
    17041 cacggaccag tgtcacccat ctgccagaag ttgtcagatg cgtatggcgc acctttgtta
    17101 tcgccgatgc gaataatacg ttcgcgcggg atcccgactt ctttttccca gatttcgtag
    17161 gcttcgtcgt cgctttcata gacggtaacc cacagacgct ctttcggcag ggcaaaccat
    17221 ttttcgctgg tcagcagttc ccatgcaaac tgaatggcat cgtgtttgaa atagtcgccg
    17281 aagctgaagt tgcccagcat ttcgaagaag gtatggtgac gcgcggtgta accgacgttt
    17341 tccaggtcgt tgtgtttacc acccgcacgc acgcagcgtt gggaagtggt agcgcgggaa
    17401 taattacgct tgtcgagccc aaggaacaca tccttgaact gattcatccc ggcgttggta
    17461 aacaacaaag ttgggtcgtt atggggtacc agggagctgc tggcaactac ctgatgtccc
    17521 ttactatgga aaaagtcgag aaacgcctga cggatctcag cggtgctctt gctcataatt
    17581 atcctgaaat caagctaacg aaatatcgct accagctcca gcgtgtctta accgccgggc
    17641 tggtaactga aaagtgggaa taagataagt tttcttgact gggaagtaaa ataccgtatg
    17701 cgttcagtcg gcaaaatttc gccaaatatc ctggatatct tccatcagat agccacgata
    17761 gagcagaaaa cgctggatct taactttttc tgaaaagaca gttggcaaag gttcatcata
    17821 ttttcgcgtc gcctgatcgc gcgccagtgc gcaccagtcg atgtcacatt cacgcatcgc
    17881 tttttctgtc gcttcgcggg aaataccttt ctgattcagt tcctgacgaa tacgtgcagg
    17941 tccataacct ttgcggctac ggctggcgat aaagcgcgca acaaatcggc tgtcatcgag
    18001 atagccatgt tcatggcacc atgcaataac gcgctcgtaa tcttctgccg tagcatcaat
    18061 ctcttctggg ccatttttgc ccataatcgg tgccgcgagt ttacgtcgca gttcttgctc
    18121 actgtgatcg cgcaccgcca gaatgcgtac cgcacgatcc aacaggcgag catatgccgg
    18181 gcgacgggac gttgattctg tcatggcata tccttacaac ttaaaaaagc aaaagggccg
    18241 cagatgcgac ccaggttttg ctgataaaca gatttaatag tcaatgagtg tgcagaatgt
    18301 tcccgcaggc ccaattcgtt agaggatcga ggtgagagta atttttcacg ccagtgctgg
    18361 cctgttcccc tcaccctaac cctctcccca aaggggcgag gggaccgtcc gtgcacacat
    18421 tatctattat catcaataac gaacgattaa aaatcttcgt tagtttctgc tacgccttcg
    18481 ctgtcatcta cagagaaatc cggcgttgag ttcgggttgc tcagcagcaa ctcacgtact
    18541 ttcttctcga tctctttcgc ggtttccggg ttatctttca gccaggcagt tgcattcgct
    18601 ttaccctgac cgatcttctc acctttgtag ctgtaccacg cgcctgcttt ctcgatcagc
    18661 ttctctttta cgcccaggtc aaccagttcg ccgtagaagt tgataccttc gccgtagagg
    18721 atctgaaatt cagcctgttt aaacggtgca gcgattttgt tcttcaccac tttcacgcgg
    18781 gtttcgctac ccaccacgtt ttcgccctct ttcaccgcgc cgatacgacg gatgtcgaga
    18841 cgaacagagg cgtagaattt cagcgcgtta ccaccggtag tggtttccgg gttaccgaac
    18901 atcacaccaa ttttcatacg gatctggttg atgaagatca gcagcgtgtt ggactgcttc
    18961 aggttacccg ccagcttacg catcgcctgg ctcatcatac gtgccgcaag gcccatgtga
    19021 gagtcgccga tttcgccttc gatttccgct ttcggcgtca gtgccgccac ggagtcaacg
    19081 acgataacgt ctactgcacc agaacgcgcc agggcgtcac agatttccag tgcctgctcg
    19141 ccggtgtccg gctgggagca cagcaggttg tcgatatcga cgcccagttt acgtgcgtag
    19201 attgggtcca gcgcgtgttc agcatcgata aacgcacagg ttttaccttc acgctgcgct
    19261 gcggcgatca cctgcagcgt cagcgtggtt ttaccggaag attccggtcc gtagatttcg
    19321 acgatacggc ccatcggcag accacctgcc ccaagcgcga tatccagtga aagcgaaccg
    19381 gtagagatgg tttccacatc catggaacgg tcttcaccca ggcgcatgat ggagccttta
    19441 ccaaattgtt tctcaatctg gcccagtgct gccgccaacg ctttctgttt gttttcgtcg
    19501 atagccattt ttactcctgt catgccgggt aataccggat agtcaatatg ttctgttgaa
    19561 gtaattatac tgtatgttca tacagtatca agtgttttgt agaaattgtt gccacaaggt
    19621 ctgcaatgca tacgcagtag cctgacgacg caccgcatca cggtcgccgc tgaagcattc
    19681 ccgccgggta atgccttcac cgcgggcagt ggcaaaagca aaccagacgg tgccgacagg
    19741 cttctcttca ctgccgccat ccggcccggc gataccacta atagacacgg cataatcagc
    19801 acgagccgct ttcagtgcgc ctatcaccat ttccaccacg acgggttcac tcaccgcgcc
    19861 atgctgcgcc agcgtctctt cgcgtacgcc gatcatctgc gctttggctt cgttactgta
    19921 ggtgacaaat ccgcgttcaa accaggcgga gctaccggca atatcggtaa tcactttcgc
    19981 tacccaacca ccggtacaag actcggcagt tgttacggtt gcgccacggg ctttcagcgc
    20041 ctgcccaacc tgttcactta actgcatcag ttcactgtca gtcatcacaa cctctgtcag
    20101 tcaaaaattt atgccggaca agatagcact ttcgtcagga agatggtgtt ggcgttgcaa
    20161 ttttacgagg aggattcaga aaaaagctga ttagccagag ggaagctcac gcccccctct
    20221 tgcaaatagt tactgcacgc gcgccagcgc cacggcttgt cctaactgcc agaccgccat
    20281 tgcgtaatgg gtgctgtggt tataacgggt aatggtgtag aagttcggca gaccgtacca
    20341 gtactggtaa ccggtaccga catccagacg cagcaggctg gcttgttgat ggttgcccag
    20401 cggctgctgt ggcgttaaac ctgccgtggc aagctgcgaa atgctgtacc tggttttgaa
    20461 gccatttggc aagcctggag cctgaccgtt tgccattacc gcgacctgat cgcctttcac
    20521 ccagccgtgc gctttgaaat agttcgccac gctaccgatc gcatcaaccg gatcccacag
    20581 gttgatatgc ccgtcgccgc tgaaatctac cgcatattgt ttgtaagacg acggcataaa
    20641 ctgtccgtag cccatcgccc cggcaaagga acctttcaga ttgagcggat cgtcctgctc
    20701 gtcgcgcgcc atcagcagga aggtttccag ctcgccagag aaatactccg cgcggcgtgg
    20761 gtagttaaat gacagcgttg ccagcgcatc gaggatgcga gttttcccca tcacgcgccc
    20821 ccagcgggtt tcaacgccga taatcccgac gataatttcc ggcggtacgc catacacctg
    20881 ccacgcgcga ttcaacgcat cttcatactg attccagaaa accacaccgt tctgcacgtt
    20941 gtccggcgta ataaattttt tgcgataacg cagccatgcg ccgttcggac ctgatggcgg
    21001 tttcaccgat gtggttggtg cctggttatc catcagccgc agtaccgaat ccagacgctt
    21061 cgcctgggag agaatttcct gcaactgctg acgatcgaaa ccgtgtttgt tcaccatttt
    21121 gtcgatgaac tgctgggcat tcgggttatt agcgaaatcg ccgcccatct gcatcacatt
    21181 gtgctgcggc tcaagcagga agccgccaga cggcgttccg gtggtcgttt cagtctcagt
    21241 aggttttggc ttgctgctac aggcggcaag caacacaaaa aggggaagca atgttacata
    21301 acgacgcttg aacatgaggg gtccatttaa cagattcaac caggggcaag tatggtaaag
    21361 catcacgccc cgcacaagga agcggtagtc actgcccgat acggacttta cataactcaa
    21421 ctcattcccc tcgctatcct tttactcaaa cttccaaatt aaaatattta tctttcattt
    21481 ttagattaaa ataacacttt taaatctttc aatctgatta gattaggttg ccgtttggta
    21541 ataaaacaat aaatcctgaa ggagagaaca atgatagaaa ccattactca tggtgcagag
    21601 tggtttatcg ggctgttcca aaagggcgga gaggtgttta ccgggatggt gaccggcatt
    21661 cttccgctgt tgattagcct gctggttatc atgaacgcac tgattaattt tatcggtcag
    21721 catcgtattg aacgttttgc tcaacgttgc gccggtaacc ctgtttcccg ttacctgctg
    21781 ctaccgtgca ttggcacgtt tgtcttttgc aatccgatga ccttaagcca gggtcgcttt
    21841 atgccggaaa agtacaaacc cagctactac gcggcggcct cttatagctg ccactcaatg
    21901 aatggcctct ttccccatat caaccctggc gaactgtttg tttatcttgg cattgccagc
    21961 ggtctgacaa cgctgaacct gccacttggc ccactggcgg tgagctatct gctggttggt
    22021 ctggtcacca acttctttcg cggctgggtg accgatctga ccaccgccat ttttgagaaa
    22081 aagatgggca ttcaacttga acaaaaagtt cacctggcag gagcaacatc atgacgcgta
    22141 ttcggatcga aaaaggaacg ggtggctggg gcggcccgct tgagctggaa gccacgccgg
    22201 gcaaaaaaat cgtctatatc accaccggta cccggcctgc gattgttgac aaactggcac
    22261 aacttactgg ctggcaggct attgacggat ttaaagaagg tgaacccgcg gaggcggaaa
    22321 ttggtgtcgc ggtaatcgac tgtggcggca cattacgctg cggcatctat ccgaaacgac
    22381 gtattcccac cattaatatc cactcgacgg gcaagtccgg tccgctggcg cagtacattg
    22441 tggaagatat ttatgtctct ggcgtaaaag aagaaaacat cactgtagtg ggtgatgcga
    22501 caccacaacc ctcttccgtg ggccgtgact atgacaccag taagaaaatc accgaacaaa
    22561 gcgatggttt actggcgaag gtgggaatgg gcatggggtc cgccgttgcg gtgctgtttc
    22621 aatctggtcg tgacaccatc gacactgtat taaaaaccat tctgccgttt atggcattcg
    22681 tctcggcgct cattggcatc attatggctt ctggccttgg tgactggatt gcccacggtc
    22741 ttgctccgct ggcgagccat ccactgggtc tggtcatgct ggcgctcatc tgctccttcc
    22801 cactgctttc acctttcctc ggcccaggcg cagttatcgc acaggttatc ggcgtattga
    22861 ttggcgtgca gattggtctc ggcaatattc cgccgcatct ggctttaccg gcactgtttg
    22921 ccatcaacgc gcaggcggcc tgcgacttca tcccggtcgg tttgtcgctg gcggaagccc
    22981 gtcaggacac ggttcgcgtc ggtgtccctt ctgtactggt gagccgcttt ttaaccggcg
    23041 caccgactgt actgatcgcc tggtttgtct ccggttttat ctatcaatag aggctgaaac
    23101 atgaccgtta tttataagac caccatcacc cgtatcggcg cgagtgccac tgacgccctc
    23161 agcgaccaga tgctcatcac ctttcgtgaa ggcgcgccag cggacctcga agagtattgc
    23221 ttcattcatt gccacggcga gttgaaaggt gcactccatc ccggtttgca atttacgctc
    23281 gggcagcatc gctatccggt gaccgctgtt ggcagcgtgg cggaagacaa ccttcgcgaa
    23341 ctgggtcatg tcaccctgcg cttcgatggt ttaaacgaag cggaatttcc gggcactgtc
    23401 catgtggcag gccctgtacc cgacgatatc gcgccgggat cggttttgaa gtttgaatct
    23461 gttaaggagt aaaaaatgaa tcaggttgcc gttgtcatcg gtggtgggca aaccttaggc
    23521 gcgttcctgt gccacggtct ggttgccgag gggtatcgcg tcgcggttgt cgatattcag
    23581 agcgacaaag ccgcaaatgt ggcacaagaa attaacgccg aatatggtga aagtatggcg
    23641 tacggttttg gtgctgacgc cactagcgag caaagcgttc tggcgctctc tcgtggggta
    23701 gatgaaatct ttggtcgcgt ggatttgctg gtctatagcg ccggaatagc aaaagcagcc
    23761 tttatcagcg acttccagct cggcgatttt gaccgttcgc tacaagtgaa tctggtgggt
    23821 tatttcctgt gtgcccgtga attttcgcgt ttgatgatcc gcgacgggat tcagggtcgc
    23881 attattcaga tcaactcgaa atccggcaaa gtgggcagca aacacaactc tggctacagc
    23941 gcagcgaaat ttggtggcgt cgggctgact caatcactgg cgctggatct ggtggagtac
    24001 ggcattacgg tgcattcact gatgctcggt aacctgctga aatcgccgat gttccagtca
    24061 ctgttgccac aatacgcgac caagctgggt atcaaaccgg atcaagtcga gcagtattac
    24121 attgacaaag taccgctcaa acgcggctgc gattatcaag atgtgctgaa tatgctgctg
    24181 ttctacgcca gtcctaaggc gtcgtactgc accggacagt cgatcaatgt caccggcggt
    24241 caggtgatgt tctgatcaac agcggagatc cattaaggat ctccgtgaga ctatagaatg
    24301 cctgatgcgc tacgctcatc aggcatacag gacttccgcc actacattaa ggaaaagtta
    24361 tggtatccgc actcatcacc gtcgccgtta tcgcctggtg tgcgcaactg gccttaggcg
    24421 gctggcaaat ttctcgtttt aaccgtgcct tcgacacact atgccagcaa gggcgggttg
    24481 gcgtgggtcg ttccagcggg cgctttaaac cgcgggtcgt ggtcgccatc ggctggacga
    24541 tcagcagcgc atcgtcgaca ccttgtttat gaaaggactg accgtcttcg cccgaccgca
    24601 aaaaattccc gcaattaccg gtatgcatgc gggtgattta cagcccgatg tgatctttcc
    24661 ccatgatcca ctatcacaga atgctctatc attggcgctt aaactgaaac gtggataatt
    24721 tcgttgtgaa tgttacttgc ttgcgaagtt atcattttga aacctaaatc aggtaatcac
    24781 gcccatgaaa cctcgtcagc gtcaggccgc cattctggag tatctgcaaa agcagggtaa
    24841 atgctcggtt gaagaattgg cgcaatactt tgacaccaca ggcacaacca ttcgcaaaga
    24901 tctggtcatt ctggaacatg ccggaaccgt cattcgtact tatggcggag tggtgttgaa
    24961 taaagaggaa tccgagccgc ctatcgatca taaaacactc atcaacaccc acaagaaaga
    25021 gctgattgca gaagctgccg ttagttttat ccatgatggc gattcgatca ttcttgatgc
    25081 tggcagtacc gttttgcaga tggttcccct gctctcgcgc tttaataaca tcacggtgat
    25141 gaccaacagc ctgcatatcg tcaatgcgct atccgaactg gataacgaac aaactatcct
    25201 gatgccaggc ggaacgtttc gtaaaaaatt ggcctcattt cacggacagc tggcagagaa
    25261 tgccttcgaa catttcagtt tcgataaatt gtttatgggc accgacggca tcgatctcaa
    25321 tgcgggcgta accaccttca acgaggttta taccgtcagt aaggcaatgt gcaatgccgc
    25381 gcgcgaagtg attttgatgg cggactcatc aaagtttggc cgtaaaagcc ccaacgtagt
    25441 ttgcagtctt gaaagcgtcg ataagctgat taccgacgca ggtatcgatc cggcgtttcg
    25501 tcaggcgctg gaagagaaag ggatcgatgt gatcataacc ggagagagca atgagtgaag
    25561 cactactgaa cgcgggacgt cagacgttaa tgctggagtt gcaggaagca agccgtttac
    25621 cggaacgtct gggcgatgat tttgttcgcg ccgccaatat catcctgcac tgcgaaggca
    25681 aagtggtggt ttcgggaatt ggcaaatcgg gccacattgg taagaaaatc gccgcaacac
    25741 ttgccagtac cggcacaccg gctttttttg tccatccggc agaagcactg cacggcgatc
    25801 tggggatgat tgaaagccgc gatgtgatgc tgtttatctc ttactccggt ggcgcgaagg
    25861 aactggatct gattattccg cgtctggaag ataaatctat cgcgctgctg gcgatgaccg
    25921 gcaaaccgac gtcaccgctg ggcctggcgg caaaagcggt gctggatatc tccgtagaac
    25981 gcgaagcctg cccgatgcac cttgcgccga cctccagcac cgtcaatacc ctgatgatgg
    26041 gtgacgcgct ggcgatggcg gtcatgcagg cgcgcggatt taatgaagaa gattttgccc
    26101 gctcccaccc agccggggca ctgggcgctc gcttgctgaa taaagtgcat catctgatgc
    26161 gccgtgacga tgccatccca caggtggcgt taaccgccag cgtgatggat gcgatgctgg
    26221 aactcagccg caccggtctg gggctggtgg cggtatgtga cgctcaacaa caggtacaag
    26281 gcgtctttac cgacggcgat ttacgtcgct ggctggtagg cggcggcgca ctcaccacgc
    26341 cagttaatga agcgatgacg acaggcggca ccacgttaca ggcgcaaagt cgcgccatcg
    26401 acgccaaaga gatcctgatg aagcgcaaaa tcactgccgc accggtggtg gatgaaaacg
    26461 gcaaactcac cggcgcaatt aacctgcagg atttctatca ggccgggatt atttaatcct
    26521 tcattcccag acgtttcgcc agccgatgca ggttggcgac gtcggtttcc agcatccgcg
    26581 cgcaggcagc ccagttatga tgattttgtg ccagtgcctg gcgaatagtt tcacgctgga
    26641 acgcttctgt cgcttcacgc aggttttgct taacaacggg caccgccgcc gcttctggcg
    26701 gcggcaacgt cacctcagga aaagcaaaat gttgcgcctc aagaatcact tcatcgccgt
    26761 tgcgggtggc tctcgccagc actaccgccc gatgaatagc atgttccagt tcgcgcacgt
    26821 tccccggaaa acgatagtgt tgcagtaaat ttcgcgctcc ggcacttaat accacgcggg
    26881 agagccccag ccgcaaacga cactgttcgc agaaataccc cgccagcaga atgacatcat
    26941 cgccccgctc acgcagcggc ggcaccgaaa gtggaaacac gctcaggcga tgaaacaagt
    27001 cagcgcgaaa tcgccctgcc agcacctctt cgcgtaagtc gcggttagtc gccgccagca
    27061 cgcgcacatc gacccgcaaa ctacggtcat cgccaacgcg ctgaatatcg ccatactgca
    27121 acaccctcag cagcttggcc tgcaatgcca acgacaactc gccgatctca tccagaaaca
    27181 gagtgccgtt atccgccatt tcaaacttcc cgctgcggtt actgatagcg ccagtaaacg
    27241 ctcctttcac atgcccgaac aactcacttt ccgccacact ttccggcagt gcagcacagt
    27301 tgagatagac cagcggattc accgcccgtg gcgaggcttc atgaatcgct ttcgccacca
    27361 gctccttacc ggttcccgtc tcaccgctga tcaggacgtt gagatcggac gccgccacaa
    27421 tctcaatctc ttttttcagt tgcgtcatgc caggggacaa gccaatcatc tgcgtctgtt
    27481 tcaccgcttc aaacggcgtg gcatcgcctg gcatcatatt ctggctttcc agttgttcaa
    27541 tcagcaacgc attgcttaac gctcccgccg ccagcgcagc aatcagccgt agctcttcgt
    27601 cgctgaaaac atcgaactga tcgggctgca tcccgtcgag cgtcagtgcg ccgatcaggt
    27661 tttgcccggc aaacaatggc agaccaacgc aggcgtgaac cttcagactc tcctgcccag
    27721 gaatcaaacc gtcataggga tcgggcaatt cgctgtctgc gggaaagcgc accacatccc
    27781 cggcgcgggc aatcgcttcc agccgtggat gcccttccag cgcaaagcgt ctaccgagta
    27841 caccctttgc cagaccgtcg atggcaagcg gaataaactg ccgcgaatcg taacgtagca
    27901 acgcagacgc atcgcactcc agcacctgac gtagcgtggt gatcaggcgc tgaaaacgat
    27961 cctgatgacc aatcccacgc tgcaattcga tggcgatatt cgccagcaca tcaacggaaa
    28021 aactcatctt tgcctcactg tcaatttgac tatagatatt gtcatatcga ccatttgatt
    28081 gatagtcatt ttgactactc attaatgagc ataattttat ttatagagta aaaacaatca
    28141 gataaaaaac tggcacgtaa tctgcaatta gcaagacatc tttttagaac acgctgaata
    28201 aattgaggtt gctatgtcta ttgtggtgaa aaataacatt cattgggttg gtcaacgtga
    28261 ctgggaagtg cgtgattttc acggtacgga atataaaacg ctgcgcggca gcagttacaa
    28321 cagctacctc atccgcgaag aaaaaaacgt gctgatcgac accgtcgacc ataaattcag
    28381 ccgcgaattt gtgcagaacc tgcgtaatga aatcgatctg gcggatatcg attacatcgt
    28441 gattaaccat gcagaagagg accacgctgg ggcgctgacc gaactgatgg cgcaaattcc
    28501 cgatacgccg atttactgta ctgccaacgc tatcgactcg ataaatggtc atcaccatca
    28561 tccggagtgg aattttaatg tggtgaaaac tggtgacacg ctggatatcg gcaacggcaa
    28621 acagctcatt tttgtcgaaa cgccaatgct gcactggccg gacagcatga tgacttacct
    28681 gacaggcgac gcggtgctgt tcagtaacga tgctttcggt cagcactact gcgacgagca
    28741 tctgttcaac gatgaagtgg atcagacgga gcttttcgag cagtgccagc gttactacgc
    28801 caatatcctg acgccgttca gccgcctggt aacaccgaaa attaccgaga tcctgggctt
    28861 taacttacca gtcgatatga tagcgacctc tcacggcgtg gtatggcgcg ataacccgac
    28921 gcaaattgtc gagctgtacc tgaaatgggc ggctgattat caggaagaca gaatcaccat
    28981 tttctacgac accatgtcga ataacacccg catgatggct gacgctatcg cccaggggat
    29041 tgcggaaacc gacccacgcg tggcggtgaa aattttcaac gtcgcccgaa gcgataaaaa
    29101 cgaaatcctg accaatgtct tccgctcaaa aggcgtgctg gtcggcactt cgacgatgaa
    29161 taacgtgatg atgccgaaaa tcgccgggct ggtggaggag atgactggtt tacgcttccg
    29221 taacaaacgc gccagtgctt tcggctctca cggctggagc ggcggtgcgg tggatcgtct
    29281 ttccacgcgc ctgcaggatg cgggtttcga aatgtcgctt agcctgaaag cgaaatggcg
    29341 accagaccag gacgctctgg agttatgccg tgaacacggt cgcgaaatcg cccgtcagtg
    29401 ggcgctcgcg ccgctgccgc agagcacggt gaataaggta gtcaaagaag aaacctctgc
    29461 caccacgacg gctgacctcg gcccacggat gcagtgcagc gtctgccagt ggatttacga
    29521 tccggcaaaa ggcgagccaa tgcaggacgt tgcgccagga acgccgtgga gtgaagtccc
    29581 ggataacttc ctctgcccgg aatgctccct cggcaaagac gtctttgaag aactggcatc
    29641 ggaggcaaaa tgagtaacgg cattgtgatc atcggttcgg gcttcgccgc ccgccaactg
    29701 gtgaaaaata ttcgcaaaca ggacgccact attccattaa ccctgattgc cgccgacagc
    29761 atggatgagt acaacaaacc tgacctcagc catgttatca gtcaggggca acgtgccgat
    29821 gaccttaccc gccagacggc gggtgaattt gccgagcagt ttaatctgca cctgtttcca
    29881 caaacctggg tgacggatat cgatgccgaa gcccgtgtgg tgaaaagcca gaataatcag
    29941 tggcaatacg acaagctagt actggcaacc ggtgccagtg cctttgtccc gcctgtgcct
    30001 gggcgtgagt taatgctgac gttaaatagt cagcaagagt atcgcgcctg tgaaacgcaa
    30061 ctgcgggatg cccgacgcgt gttgattgtt ggcggtggtt tgattggtag cgaactggcg
    30121 atggattttt gtcgtgcagg caaagcggtc acgctaatcg acaacgctgc cagtattctg
    30181 gcgtcgttaa tgccaccgga agtaagcagc cgcttgcagc atcggttgac ggagatgggc
    30241 gttcatctgc tgttgaaatc tcagttacag gggctggaaa aaacggattc tggcattctg
    30301 gcaacgctgg accaccagcg tagtatcgaa gtggatgcgg taattgccgc caccggactg
    30361 cgcccggaaa cagccctggc acgacgcgcc gggctgacga ttaatcgcgg cgtttgcgtc
    30421 gatagttatc tgcaaaccag taatgacgat atttatgcgc tgggcgatta cgcggaaatc
    30481 aacggtcagg tactgccatt cctgcagccg attcaactta gcgcgatggt gctggcaaaa
    30541 aatcttctcg gcaataacac gccgctgaaa ctaccgacga tgctggtgaa aatcaaaacg
    30601 ccggaattac cgctgcatct ggcaggcgaa acccagcgtc aggatttacg ctggcaaatt
    30661 aataccgaac gctagggaat ggtggctcgc ggtgttgacg atgctgacca gcttcgcgcc
    30721 tttgtggtca gtgaggatcg gatgaaagag gcatttggat tgttgaaaac attgccgatg
    30781 taggtgggct actgtgccta aaatgtcgga tgcgacgcta gcgcgtctta tccgacctac
    30841 ggggacgcat gtgtaggccg gataaggcgt ttacgccgca tccggcaatg gtgtccaaat
    30901 gcaacacgtt ttatccgttc tggacttcac ccgctaacca acgcgccgca gcaataaccc
    30961 cctgccccag agacaaaccg ccatcacccg ccggtaaact ctgtggaaag agcaatgtga
    31021 aatcagcgag ataatgcgcc agacgtgcac gcagcaaacg gttatgaata accccgccgc
    31081 taaataccag cgtagtgata ccacgcatcg tggcctgctc acgcatcaac gcggcaaaac
    31141 cctgcgccag cgcatcatga aacgcccacg cgcgttgatt aacctgtgcc tgccagttca
    31201 gccactgctg ccagaaagcg gcgagatcca gttgattgtc caccagcggc atcgtcaccg
    31261 gatgcgtcac tccgtggcat gaggctgcga gcgcctccag agcacaagcc gcttcacctt
    31321 cataacttaa cgtggctggc gcacagccca gtgccgccgc cactgcatcg aacaaccgcc
    31381 cacacgatga cgccagcggc gcgttaattc cacgctcaat ggcccgcgcc agcacgctcc
    31441 agttttgctg ttgcacactt gctgtttcag ggtaattctg ccactccggc acaaagcgca
    31501 ggcactgcgc cagcaggttt cgccacggct gcttcgctgc caaatcgcca cccggaagcg
    31561 ccactgcagg caagccgccc aggtgctcac attcgcgata gttcacccgc aggcactcgc
    31621 cgccccacaa agcgccgttc tcccccatac cgataccgtc gagcgtcaaa gcaatgacat
    31681 caccgccatc cagcggccac tgatgctctg ccagacacgc cgctgcatgg gcatgatgat
    31741 gcagtaccgt ttgcgtcggc agattcattt cacgcgccca ctggctggag acatagcccg
    31801 gatgcgcgtc atgcacaacg tatttcgggg taaaatcgta gatgttttgc atcaggcgta
    31861 acgcttcgcg ccactgcatc tggatgccat cgtcacttaa atcgcccaga tgctgactca
    31921 acaccgcttg ttcaccgcgc accaggcaga aggtattttt cagatccgct ccgagacaca
    31981 gcacaggcgg aacattttta aagcccggag gcaaagccag cgcatccggc acataccccc
    32041 gcgaacggcg cagcatttcg ccgctttcgc gcaccaccga atcatccatc cgctgcacga
    32101 tgtcgcggtt atgtatcaag aatccgtcgg caatgccctg caaatccgcc agcgcctgtt
    32161 cgttgctgat agctggtggt ttaccgctca ggttgccgga ggtcatcacc agcgggcatt
    32221 gcagttcctg taacagcaaa tgctggagcg ggttcgcagg caacattacc ccgacttcgt
    32281 taaggtcagg ggcgatatca tcacaaagct caggaacgta ttttttatcc accagcacaa
    32341 tcggcgcggc gggcgtggta agcaactggc gcgcagcgtc tggtaaaccg tcagccactg
    32401 gcaacatgac cgccagcggt ttcgccgggc gatgtttgcg cgcccgaagt gtcgccaccg
    32461 cgttactgtt acgtgcatcg caggcaagat gaaatccgcc aatccctttg atggcgacaa
    32521 tgttgcccat ttttaactgt gcgatagccg cctgtaatgc cgcctcttgt tctgcatgtt
    32581 caccatgact tacccattca agatgcgggc cacactccgg gcaggccacc ggctgggcgt
    32641 ggaagcgacg atcgagcggg tcgcggtact ctttgtcaca agccggacac aacggaaacg
    32701 ccgccatcac ggtaaacggg cggtcgtaag gcatggcgcg aataatggtg aaacgcgggc
    32761 cgcagtgggt acagttgata aacggataac gataacgccg ttcgcctggg gtattcattt
    32821 cggcaaggca agcagggcaa gtagcggcat ccgggacaat ttgcgtattc atggcacctc
    32881 ccgcgctctg gcggatggtg aactcagtgg gcagttgtga ccagatgtac ggctcacgct
    32941 cgacgctatc aatacgcgcc agcggcgggc agtgctgatg caattgaaca agaaacgttt
    33001 ccgggtcttc ccgcagccgg acttctacgc catcgccgtc attacagaca tcgccgtgaa
    33061 gatttaattg ctgtgccagc tgccagacaa acggacgaaa accgacgccc tgcactttgc
    33121 cacgaatacg cagttggaca ccgcaagatg tgttttttgc cattgagtta ttcccgccat
    33181 catgaattgc gtaacccgcc cagccggaca cgacagcgtc gcatccggca gtcacaggtc
    33241 ggcgataccg cctgctccgt attctacgaa tatttccggg aattcctttg atgccagaac
    33301 agttctgtaa gatttttaga acatcagcgc cgtacggcgg cgtttttctg cactcagttg
    33361 ttcaagttta ttacgatcga cacaaatcag cgcatgagtc gggcaagccg ccatacacgc
    33421 cgggccgtct tcacgatggt tgcacaggtc acatttattg gcttcggctt tgtcagcccg
    33481 tacattcagc cccgcgccgc tgttgcgaat caccggacgt accaccactt ccatcgcgcc
    33541 atacgggcaa gccacaacgc aggttttgca accaatgcaa cgttcctgca tcacatgaac
    33601 aaacccttta tcacggctga tagcaccatt cgggcagacg ttagcgcacg gtgcatcttc
    33661 acactgacgg caaactgtcg ccgtggaaat gttcacacct ttaatgacat ggatacgcgg
    33721 taaaaatgtt tccggggtca gcgatgcaca gtcctgattt tcctgatgag aaaccacgca
    33781 cgccacttca caggtacggc aaccaataca tttactcgcg tcagcaatga tgaaacggtt
    33841 catcaaattc tccagcaatg acagttaatg cgccgataca ttcacaaatc atgccagttt
    33901 ttaatttact gttatttaag gaaattaatt tctgtaatgc aggaaaaaag atgtcatcga
    33961 cactagtgac gatgacatgt gatgacaatg tttatcgcga aagagcaatg agtgagtctc
    34021 ggtggatcag ttttccgctg aaggttttcg gcggtgagaa atccccgcca tcgagcataa
    34081 aaatcagccg tccaataatt tcctgaatca tctcagtcac cggaattttt acgctggaga
    34141 gcgccggaac gatatagggg gcaattgcga tatcatcgaa tccgataact gacacctgct
    34201 ctggcaccgc tacgccgcgc tcgtgcaacg ctttcatcgc acctatcgcc atatcgtcgt
    34261 tactggcaac taacgcgcta aatttagccc cacgttcgag caacgtttct accccttcgg
    34321 ccccgctggc gggcgtccat ttaccgttag cgataagttt ttcattgagc gcaataccat
    34381 gctgcgccag cgcgtcttta tacccagcaa gacgttcaat gctggtgggg gaatccatcg
    34441 agccggtaag gaaagcaatc tcctgatgtc cggcgtttat caactctgcc acggcattaa
    34501 agctggtctg tttatgatcg caccagacgc tatggctgct gtttttgcgc aggcggcgat
    34561 taagcaccat tatcggctga ctgtgcgcgt caatgatgtc atcgatctca tccacgctta
    34621 aaaaacgcgg gtaaatcata atggcgttgc agcgcaaatc cagcagatac tgaatcgcct
    34681 ggcgctcttc ttcggcgctg tgtttaccat ctgccaatag caactgccgc cctttctctt
    34741 ccgccattcg cgcggcatga aagagtaatt cactaaaata aatgccgtgg taaagcgtgt
    34801 tggtcactac cagccccagc gtctgagtac tcttcgccga cagattgcgc gccagcaagt
    34861 ttgggcggta accgctctct tctaccgcct gaaacacgcg atctttagtc tcctggctga
    34921 cgtagccatt acctgaaagc acgcgggaaa cggtcgcttt tgaaaccccg gcgcgcttcg
    34981 ccacttccag catcgtcgtc atcattttca tccctttaca cgcaatcaac gcagtgtact
    35041 gcaccgtttg ccgattgtcc ttgcgcaatc ggcgggaaaa atattcaggt gaccggtttc
    35101 acaaatataa aaaatgaata attaactctc ttgcttattt agtgacaact attcatgatt
    35161 ttgtgaaacc ggtttcctaa ttccgtttca gcatcggcat ttttccgtta cgtcgactga
    35221 taacaactac atctacccta ctgataacag gataaaatcc gatggccaaa aattatgcgg
    35281 cgctggcacg ctcggagata gcggcactgg gcggcgttga taacatctcg gcggtcacgc
    35341 actgtatgac gcgtttgcgc tttgttatca aagatgacca gcttatcgac agcccgacgt
    35401 taaaaaccat ccccggcgtg ctcggcgtgg tacgtagtga caaccagtgt caggtgatta
    35461 tcggcaatac cgtttcacaa gcctttcagg aagtcgtcag cctgctgccg ggagatatgc
    35521 agcccgcaca gcccgtgggt aaacccaaac tcacgccgcg tcgcattggt gcggggatcc
    35581 tcgatgcgct gatcggcacc atgtcaccgc tgatcccggc gattatcggc ggatcgatgg
    35641 tcaaactgct ggcgatgatc ctcgagatga gcggcgtgct gacaaaagga tcgccgacct
    35701 taaccattct gaatgtgatt ggtgacggtg ctttcttctt cctgccgctg atggtcgcgg
    35761 catctgccga catcaaattt aaaaccaata tgtcgctggc gattgccatc gcgggtgtgc
    35821 tggtacatcc gagttttatt gaactgatgg cgaaagcggc ccagggtgaa catgtcgaat
    35881 ttgccctgat tccggtcacc gcggtgaaat acacctacac agtgatcccg gcgctggtga
    35941 tgacctggtg cctgtcatat atcgaacgct gggtggatcg cattacaccg gcggtgacca
    36001 aaaacttcct caagccaatg ctgattgtgt tgattgccgc accgctggca atcctgctga
    36061 ttggcccgat tggtatctgg atcggtagcg ccatttcggc gctggtttac agcattcatg
    36121 gttatctggg ctggctttca gtcgccatta tgggcgcgct gtggcctctg ctggtaatga
    36181 ccgggatgca ccgcgtcttt acgccaacca tcattcagac cattgccgaa accggcaaag
    36241 aagggatggt catgccgtca gagatcggcg ctaacctgtc gctgggcggt tcatcactgg
    36301 cggtggcgtg gaaaacgaaa aacccggaac tgcgccagac ggcgctggct gcggcggcat
    36361 cagccattat ggcggggatt tccgaaccgg cgttatacgg cgtggcgatc cgcctgaaac
    36421 gtccgcttat cgccagtctt atcagcggtt ttatttgcgg cgcggttgcc ggtatggcgg
    36481 ggcttgccag ccactcaatg gcagcgccgg ggctatttac cagcgtgcaa ttcttcgatc
    36541 cggcgaatcc aatgagcatc gtctgggtgt tcgcggtcat ggcgctggcg gtggtgctgt
    36601 cgtttatcct cacactgttg ctcggctttg aggatattcc tgttgaggaa gcggctgccc
    36661 aggcgcgaaa gcatcagagc gtacaaccga ccgtcgccaa agaagtaagt cttaattgag
    36721 gatgaaaatg tcagtatttc cagaaggttt tttatggggc ggcgcgcttg ccgccaacca
    36781 gtctgaaggt gcgttccgtg aaggcggaaa agggctgacc accgtcgata tgatcccaca
    36841 cggcgagcat cgaatggcgg tgaaactggg gctggaaaaa cgttttcagt tgcgcgatga
    36901 cgagttttat cccagccatg aggcgacgga tttttatcat cgttataaag aagatatcgc
    36961 cctgatggca gagatgggat tcaaggtttt ccgtacctca attgcctgga gccgtctctt
    37021 tccgcagggc gatgaaatca cgcccaatca gcagggcatt gctttttatc gttctgtctt
    37081 tgaagagtgt aaaaagtacg gtatcgaacc gctggtcacg ttgtgccact tcgatgtgcc
    37141 gatgcatctg gtcaccgaat atggctcctg gcgtaaccgc aagctggtgg agtttttcag
    37201 ccgctacgcc agaacctgct ttgaagcatt tgatggtctg gtgaaatact ggctaacctt
    37261 caatgaaatc aacattatgt tgcatagccc gttctccggc gcgggtctgg tgtttgaaga
    37321 aggtgaaaat caggatcagg tgaaatatca ggccgcgcat caccagctgg ttgccagtgc
    37381 gctagccgcc aaaatcgccc atgaggttaa cccgcaaaat caggtgggct gtatgctggc
    37441 gggcggcaac ttctaccctt acagctgcaa accggaagat gtctgggcgg cgctggagaa
    37501 agatcgggaa aacctgtttt ttatcgatgt gcaggcgcgc ggcgcgtatc cggcttactc
    37561 tgcccgcgta tttcgcgaaa aaggggtaac catagataaa gcgctgggcg atgatgagat
    37621 cctgaaaaac accgtcgatt ttgtctcttt cagctattac gcctcgcgct gcgcctcggc
    37681 ggagatgaac gccaacaaca gcagtgcggc gaacgtggtg aaatcgctgc gtaacccgta
    37741 tctgcaagtg agcgactggg gctggggaat tgatccactc ggtctgcgta tcaccatgaa
    37801 tatgatgtac gatcgttatc agaagccgct gtttctggtg gaaaacggcc tcggcgcaaa
    37861 agatgaactt gctgccaatg gcgagattaa cgatgactat cgcatcagct atttacgcga
    37921 acatatccgc gcaatgggcg aagcgattgc agatggcatt ccgctgatgg gctacaccac
    37981 atggggctgt attgatttag tttccgcctc tacgggtgaa atgagcaaac gctacggttt
    38041 tgtctacgta gaccgtgacg acgcaggcaa cggcacgctg acgcgcacac gtaagaaatc
    38101 gttctggtgg tataaaaaag tgattgccag taatggggaa gatttagagt aagtaacagt
    38161 gccggatgcg gcgtgaacgc cttatccggc ctacatgtcg cacgaaccag gaaggcccga
    38221 taagcgcagc gcatcgggca atttagcatt tggcatcaga gctggttcac cccatcaaga
    38281 acatccctgt cctgattcct taatgaaaaa ctactcttct tccaccgcta actgcgcgaa
    38341 gccgccattc ccttcccagc cttccagtcg ttgataaacg gtttctaccg catctttaat
    38401 cggctgggtc atcgggtagt aaaagccgac gatatccggc tgtatgccga ggaaaatcac
    38461 ttcgccaata tcttctttca actggtcgat aagatagttc agaggcatgt tatgggtagt
    38521 catcataaac atctcggcga tatcatccgg gtcgatgatg cggatctcgc cggggtttag
    38581 ccccatatcc gtggcgtcga caatcagtaa ccgcgtaggg cgcagttcac ggatggcgac
    38641 gatgtcgttt tccggtgcac taccgccgtc aatcaccacc cagttacctt tcggcgcggc
    38701 ggcgcacttt tccgccagca gcggacctgc gccatcatcg cccatcatgc tattgccaac
    38761 acagagtaaa acgtcagtca cgcaatctcc tcaccatcaa atagatggcg ttttcctgat
    38821 gaatatcatg cagcatactt aacagcattg tactccattc ctgctgctgc gaagtttgcg
    38881 ctgcccgggc tttatcgaat gcgttggcca gcatcggcac atggttaatg tcgatgacga
    38941 tctcaccgta cttcggtacg ccttccattt tgcggcgggc ttcactgccc gcctccagag
    39001 tggcaatcca tgccagatat tcatcccacg ggcaggtgag cgccgcttcc aggcaatcga
    39061 taaccccaag gtggtgacca atcgccaggc tgtaatagac cacctgctgc gcctcggcgg
    39121 gcgtggcatc gttctcatca ataaatttac ggctcagttg actgaacacc accttttcac
    39181 tcatcggata cgcacctctt ctacaacatg attcagatgg ctgacaatct cgttcagacg
    39241 cggatcgttt tccgcttcca gccagcgtgc aacctgttct tcgccctgcc ctaactgtgt
    39301 caggaagtca tcggcaatct gacgaccgta acgataaccc gccaggcggc gtgcttcgcg
    39361 atccactttc acgcgcagcg gctgcaccat atcgccatgc aggatctccg ccggttgttc
    39421 atccagttca cccggcccac gggcgtgaat tttctgctcc agcaggccga gcgccattgc
    39481 aaagccgtac agcgtggcgg caggcgttgg cgggcagcca gggatataaa catccacagg
    39541 gacaatttta tccgtaccgc cccacacgca gtagagatcg tggaagatcc cgccgctgtt
    39601 accgcaggca ccgtaggaga tacaaatttt cgggtccggc gcggactgcc acgcacgcag
    39661 cgcaggggat cgcattgcac gggtgaccgc gccggtaaac agtaaaatat ccgcatgacg
    39721 cggtgaagga acgactttaa tgccgaagcg ttctgcatca aacagcggcg ataaagtggc
    39781 gaaaatttcg atttcgcagc cgttgcagcc gccgcagtcc acgcggtaaa cataggcaga
    39841 acgtttgatt tttttcagta acgacgcctt catgctggcg atggattcat ccaccgtcat
    39901 cgggaccgga atgccgttgg cgtcacgggg gcctaataaa ttgctcatca gatggcctct
    39961 ttcatatggc gagtcagttc aatacggtcg gacggcacca ggcatttctg gcgcttacat
    40021 tccgggcaag tctcaaagct ttcgcggtgg ttttccgcgc ggctgtcgcc gttgtgctta
    40081 agcagcgcaa tggcgtagtc gatctctttc tggacggcga aaggacgatt gcagacgcga
    40141 cagttgcaca gcgcgaagcg agactgttgc aggaagtctt ctttcttcca caccgccagt
    40201 tcgtactctt gcgacagctt aatcgccgcc gtcgggcaaa cttcttcgca gcgtccacag
    40261 aagatgcagc gcccgagatt aaactcccag gccagctctc ctgtggcgag gtcagtttca
    40321 accgttaagg cgtttgacgg gcaggcattg acgcaggccg cgcagccgat gcactgctgc
    40381 gggttctgct ctggcttacc acggaagttt ttatcaaccg caatcggctc cagcggataa
    40441 gacgaggtcg ccgtaccggt tttgataact tttttgataa aggtaaacat ggcgattcct
    40501 tatttcagcg gcgagttttt acgctcaatg ctgtagcgct cgagttcttt gtacggcacc
    40561 actttgctct tcttcttacg tacatcgacc acagtcatgc ggtcggtaca ggagtagcaa
    40621 gggtccaggc taccgataat cagcggcgca tcggaaacag tgttgccacg cagcatgtag
    40681 cgcagggtcg gccagttcgc gtaggtcgcg gcacggcagc gccagcggta cagcttctgg
    40741 ttgtcgccag tcatgctcca gtggatatca tcaccgcgcg gcgcttcggc aaagcccagc
    40801 gcgaaacggt gcggaatgta ggtaaagcct tccaccatca gcgggccacc cggcaggtta
    40861 tccagaccgt agtcgatcat gttcagcgcg gtatagactt cgttgatacg cactttcaga
    40921 cgcgaaataa cgtcgcagcc ctgctcgctg tggacttcca ttggcagcag gccataacca
    40981 acaaacgggt gatcagcgcg ggtatcgcga gcgtgtccgc tggcgcggac catcgggcca
    41041 acgttactga aatcgcgggc aatttccggg tccagacgac caatgccgac ggtgcgctgt
    41101 tccatgttcg gtgtgctcag cagcacatcc accagctcct gcacttcacg acgcatctgt
    41161 tgcgccagct ggcgggtctg gatcatgtca tctttcaaca gatcgcgacg aatcccgccg
    41221 atcaggttca ggccgtaggt tttacgcgca ccggtgagga tctcggccat tttcatggag
    41281 gtttcacgca cgcggaagaa ttgcataaag ccggagtcga agccggtaaa gtggcaggcg
    41341 aggccgaggt tgagcagatg cgagtgcagg cgttccacct ccagcagaat ggcgcggatc
    41401 atctgcgcac gttctggcac ctgaataccc atcgcgtttt ccaccgacgt ggtgtaggcg
    41461 gtgctgtggg cgaagccgca gatcccgcac acacggtcag agaggaaggt cacttcgtta
    41521 taacccatgc gggtttccgc cagtttttcc ataccgcgat ggacatagaa caagcggtag
    41581 tctgcgtcga taatgttttc gccatcgacg aacagacgga agtggcccgg ttcgtcagaa
    41641 gtgacgtgca gcggaccaat cggcacgacg ttgtttttct tgtcgcccag ttcgttgatg
    41701 aactcgtagg tttcagcatc ggtggtcggt gccggacgct gacgataatc catgctgtct
    41761 ttacgcagcg gataaagttc atccggccag tcatccggca gcaccagacg gcgttcatcc
    41821 ggcagaccaa ccggaatcaa accgtacata tcgcgcactt cgcgctcgcc ccacaccgcc
    41881 gccggaacgc gcggcgtcac ggacggatac tccggtttgt tggcatcaac ttcgacgcga
    41941 accgttaccc agcacttagt ccccttctcc atcgacagca cgtaataaac ggcgtaatga
    42001 ccattcagtt tgcgttcgtc gttaccaaaa agcaccgaca gccagccccc ctgcttgtag
    42061 tagagaaact ccaccacttc cggcagatag ttcaccttta cggtgacagt cagctgatct
    42121 ttggtctgcc aggcgtggtc cagcacgacg cccggaaatg cctcattcag cgcggcgaga
    42181 taatgttgac ctaatttttc ttcagacatg ctcaaactct ctttaatcac gccgccagca
    42241 aggagacgaa cgctaaaaat gcaaagccaa acccagccca ggtaatgcgc ggagtaatat
    42301 caagacgcag acgggccatg ctgttttcga acagcgcgat aaccaggacg ccgaccacca
    42361 gttttacgat cgcaatcacc agcgccagca gcagtccacc ggcggtgaag gtttccattt
    42421 gtccccacgg aataaacacc ccgacgaaca tctgcaacac caccagctgt ttcaggctga
    42481 taccccattt catgacgcca aagccgctgc cgctatactc agagagcgga ccttcctgca
    42541 actcctgctc ggcttccgcc aggtcgaacg gcagtttgcc catttcgata aaagtggcga
    42601 acgcacaggc gcagagcgcc agcaccagcg ggatgctttg gctcagcggc cagtgataaa
    42661 cggtgtcggt aatgttgctg atgttggtgg aaccggcaac ctgtgcggca acccacagac
    42721 caagcagcaa catcggttcg accagcacgc caagcatcgc ttcacggctc gcaccgatag
    42781 cggtaaacgg gctaccggta tccagaccgg aaatggcaaa gaagaaacgc gcgatggcga
    42841 agagatacag taaggtgatc aaatcgccca gttgcggcag cggagaaccg acggtcacca
    42901 ccggcagcgc agtagcgata gtcagcatga cgcccaccat cacatacggc gtcaggcgga
    42961 acacccagcc agaggcatcc gggccaacgc tctggcggcc cagcagtttg ataatgtcgc
    43021 gatactcctg caacacgccc ggcccgcgac ggttatgcag acgggcgcgc gccacgcggg
    43081 taataccgga gagcagcggc gcaacggcaa ataacaccag cgcctgaatt aacggatata
    43141 aaacactcat tctcaggctc ctcgtgaaac aataatcacc accagtaccg ccagttcaac
    43201 cagcgccatc cggcggaaca gcaacgcact accttcgcac tgccagcccg gcaccagcga
    43261 caccggattc agccatttgc gcagtttcag cactggcgca aacgcctgtt taaccggcat
    43321 ggcaaaaccg tgagcggtaa tcaccattga tttttcatgg tcgtagccgc acacccaggc
    43381 cgcaccgcgg gaacgtgacg gcaggcggtc gcctttgcaa atcgccataa tgatgaatgg
    43441 cagcagcggg caggcaatca gcagcaatgt gatcattggt tgagaaacgg tggtgttagc
    43501 aggctccaga ggcagaggta cggcagcaga gagcatcggc agcagccacg gcgcagcaac
    43561 accgccaatt acgcagcaaa tcgccagtgc cactacgctt acgctcatca ggagcggcgc
    43621 acaggtggcg ttttcggctt ctttggtacg cggcgcgccg aggaacgtga cgccatagac
    43681 tttcgccata cacatcaccg ccagcgcacc ggtaattgcc agacccacag cgagcagcgg
    43741 ccccagcaga cgggcaacaa acgcgccact attgctcagt ttgaaaaagg attgatagat
    43801 aacccattcc ccggcaaacc cgttcagcgg cggcagcgca gccattgcca tcaacccgac
    43861 taacatggcg atggagataa ccggcatttt cttgccaata ccaccgagtt tttcgatatc
    43921 gcgatgaccg gtacggaacc agacgctccc cgccccgagg aacagtacgc ttttgaacag
    43981 gctatggtta agcagatggt acagaccacc gaccaggcca agagcaatca gcgccggttg
    44041 ttcgagcgcg atacccgtta cgccagcgcc cagccccagc aggataatgc cgatattttc
    44101 cagggtgtgg taagccagca ggcgctggat gttgtgctcc atcagcgcat acagaccacc
    44161 gacaaacgcg gtgatcatgc cgagcaccag cagcacgatc ccccaccaca gcggtgcatt
    44221 accgcccagc agtgacaggg ttaaaatgcc cagcaggcca attttcatga ctaccgtaga
    44281 aaacaacgcg gcagctggcg cagaggcgtt agcatgtgcc tgcggcaccc agccgtgcag
    44341 cggaataatc ccggcgagca ggccaaagcc aatcacaccg agcagccaga tatcggaacc
    44401 gagcggcagc tgttgcatac gcatatccag caggcgcaga tccagcgtgc cataacgctg
    44461 ccacagcagc cagcaagcaa tcgccagcag cagagtgcca agacgcccca gcgcaaacca
    44521 cagtttgccc tcttttgttt cgacgtaata ctcgatggtg tggctgttaa agccatccgc
    44581 cagggtgacg taaatacgaa aatgttcaaa aaggacgaaa cagagttctt tgtccggcgc
    44641 gcagctcatg gcgtgatgca ggcgcgcttt cgataacgtg atcccctgaa ccagcgaatt
    44701 gcagtagatg tgccactggt cctgtaggcg acgatgccgc tgtgcgatgt aatcggcttt
    44761 ctcgcttatt tcccaaatag tcattgtcag gttacccgtt taacagagat gccagcttta
    44821 agcattttct gtgccaattt ttaatttatt gttattaaaa cgatttttaa gctaaagatg
    44881 aatttcgtcg ccgtgtcgac gtgtcatttc gacatcatcg acattattca ccgcagggat
    44941 aatcaacact ggcacaatta ttgcttgtag ctggcaatag ttaatgggag gcgatatgca
    45001 cgaaataacc ctctgccaac gggcactgga attgatcgaa cagcaggccg caaaacacgg
    45061 cgcaaaacgc gtaactgggg tctggctcaa aattggcgca ttttcttgtg tcgaaaccag
    45121 ctctcttgcc ttttgttttg atctggtttg ccgcggcagc gtggcggaag gttgtaaact
    45181 gcacctcgaa gaacaagagg tcgaatgctg gtgtgaaaca tgccaacagt atgtgacgct
    45241 attgacccag cgcgtccgcc gctgtccaca gtgtcatggt gacatgctac agattgtggc
    45301 agacgacggt ttacagattc ggcggataga aatagaccag gagtgagcga tgtgtacaac
    45361 atgcggttgc ggtgaaggca acctgtatat cgagggtgat gaacataacc ctcattccgc
    45421 gtttcgtagc gcgccatttg ccccggcggc acgcccgaag atgaaaatca ccggcattaa
    45481 agcgcctgaa tttaccccca gccagactga agaaggcgac ctgcattacg gtcatggcga
    45541 agcgggcact cacgcaccgg gcatgagcca gcgtcggatg ctggaagtcg aaattgacgt
    45601 gctggacaaa aacaaccgtc tggctgaacg caaccgcgcg cgctttgctg cccgcaagca
    45661 actggtgctc aacctggttt ccagcccagg ttccggtaaa accaccctgc tgacggaaac
    45721 cctaatgcgc ctgaaagaca gcgttccgtg cgcggttatt gaaggtgacc agcaaaccgt
    45781 gaacgatgcc gcacgcattc gcgctaccgg cacaccagcg attcaggtga acacaggtaa
    45841 aggctgccat cttgacgcac agatgattgc cgacgccgca ccgcgtctgc cactggacga
    45901 taacggtatt ctgtttatcg aaaacgttgg caacctcgta tgcccggcca gcttcgatct
    45961 cggtgaaaaa cacaaagtgg cggtgctttc cgttaccgaa ggtgaagaca aaccgctgaa
    46021 atatccgcat atgtttgccg ccgcctcgct gatgctgctc aacaaagttg acctgttgcc
    46081 gtatctcaac tttgacgttg agaagtgcat cgcctgcgcc cgcgaagtca atccagaaat
    46141 tgaaatcatc cttatttccg ccaccagcgg tgaagggatg gaccagtggc tgaactggct
    46201 ggagacacag cgatgtgcat aggcgttccc ggccagatcc gcaccattga cggcaaccag
    46261 gcaaaagtcg acgtctgcgg cattcagcgc gatgtcgatt taacgttagt cggcagctgc
    46321 gatgaaaacg gtcagccgcg cgtgggccag tgggtactgg tacacgttgg ctttgccatg
    46381 agcgtaatta ctgaagccga agcacgcgac actctcgacg ccttacaaaa catgtttgac
    46441 gttgagccgg atgtcggcgc gctgttgtat ggcgaggaaa aataatgcgt tttgttgatg
    46501 aatatcgcgc gccggaacag gtgatgcagt taattgagca tctgcgcgaa cgtgcttcac
    46561 atctctctta caccgccgaa cgccctctgc ggattatgga agtgtgcggt ggtcataccc
    46621 acgctatttt taaattcggc ctcgaccagt tactgcctga aaacgttgag tttatccacg
    46681 gtccgggttg ccctgtgtgc gtactgccga tgggtagaat cgacacctgc gtggagattg
    46741 ccagccatcc ggaagtcatc ttctgtacct ttggcgatgc catgcgcgta ccagggaaac
    46801 agggatcgct gttgcaggca aaagcacgcg gtgccgatgt gcgcatcgtt tactcgccga
    46861 tggatgcgtt gaaactggcg caggagaatc caacccgcaa agtggtgttc ttcggcttag
    46921 gttttgaaac caccatgccg accaccgcca tcactctgca acaggcgaaa gcgcgtgatg
    46981 tgcagaattt ttacttcttc tgccagcaca ttacgcttat cccgacgctg cgcagtttgc
    47041 tggaacagcc ggataacggt atcgacgcgt tcctcgcgcc gggccacgtt agtatggtta
    47101 tcggcactga tgcctataat tttatcgcca gcgattttca tcgtccgctg gtggtcgctg
    47161 gtttcgaacc ccttgatcta ctgcaaggcg tggtgatgct ggtggagcag aaaatagcgg
    47221 cccacagcaa ggtagagaat cagtatcgtc gggtggtacc ggatgccggt aacctgctgg
    47281 cgcaacaggc gattgccgat gtgttctgtg tcaacggcga cagcgaatgg cgcggcttag
    47341 gcgtgattga atcttctggc gtgcacctga cgccggatta tcaacgattc gatgccgaag
    47401 cacatttccg cccggcaccg cagcaggtct gcgatgaccc gcgcgcgcgt tgtggtgagg
    47461 tattaacggg caaatgtaag ccgcatcaat gcccgctgtt tggtaacacc tgtaatcctc
    47521 aaaccgcatt tggtgcactg atggtctcct ccgaaggagc gtgcgccgcg tggtatcagt
    47581 atcgtcagca ggagagtgaa gcgtgaataa tatccaactc gcccacggca gcggtggcca
    47641 ggcgatgcag caattaatca acagcctgtt tatggaagcc tttgccaacc cgtggctggc
    47701 agagcaggaa gaccaggcac gtcttgatct ggcgcagctg gtagcagaag gcgatcgtct
    47761 ggcgttctcc accgacagtt acgtcattga cccgctgttc tttcctggcg gtaatatcgg
    47821 caagctggcg atttgcggca ccgcgaatga cgttgcggtc agtggcgcta ttccgtgcta
    47881 tctctcctgt ggctttatcc tcgaagaagg attgccgatg gagacactga aagccgtagt
    47941 gaccagcatg gcagaaaccg cccgcacggc aggcattgcc atcgttactg gcgatactaa
    48001 agtggtgcag cgcggcgcgg cagataaact gtttatcaac accgctggca tgggcgcaat
    48061 tccggcgaat attcactggg gcgcacagac gctaaccgca ggcgatgtat tgctggttag
    48121 cggtacactc ggcgaccacg gggcgactat ccttaacctg cgtgaacaac tggggctgga
    48181 tggtgaactg gtcagcgact gcgcggtgct gacgccgctt attcagacgc tgcgtgacat
    48241 tcccggcgtg aaagcgctgc gtgatgccac ccgtggtggt gtaaacgcgg tggttcatga
    48301 gttcgcggca gcctgcggtt gtggtattga aatttctgaa tcagcactgc cggttaaacc
    48361 tgctgtgcgc ggcgtttgcg aactgctggg actggacgcc cttaactttg ccaacgaagg
    48421 caaactagta atagctgttg aacgcaacgc agcagagcaa gtgctggcag cgttacattc
    48481 ccatccactg gggaaagacg cggcgctaat tggtgaagtg gtggaacgta aaggtgttcg
    48541 tcttgccggt ctgtatggtg tgaaacgaac cctcgattta ccacacgccg aaccgcttcc
    48601 gcgtatatgc taataaaatt ctaaatctcc tatagttagt caatgacctt ttgcaccgct
    48661 ttgcggtgct ttcctggaag aacaaaatgt catatacacc gatgagtgat ctcggacagc
    48721 aagggttgtt cgacatcact cggacactat tgcagcagcc cgatctggcc tcgctgtgtg
    48781 aggctctttc gcaactggta aagcgttctg cgctcgccga caacgcggct attgtgttgt
    48841 ggcaagcgca gactcaacgt gcgtcttatt acgcatcgcg tgaaaaagac acccccatta
    48901 aatatgaaga cgaaactgtt ctggcacacg gtccggtacg ccgcattttg tcgcgccctg
    48961 atacgctgca ttgcagttac gaagaatttt gtgaaacctg gccgcagctg gtcgctggtg
    49021 ggctataccc caaatttggt cactattgcc tgatgccact ggcggcggaa gggcatattt
    49081 ttggtggctg tgaatttatt cgttatgacg atcgtccctg gagcgaaaaa gagttcaatc
    49141 gtctgcaaac atttacgcag atcgtttctg tcgtcaccga acaaatccag agccgcgtcg
    49201 tcaacaatgt cgactatgag ttgttatgcc gggagcgcga taacttccgc atcctggttg
    49261 ccattaccaa cgcggtgctt tcccgcctgg atatggacga actggtcagc gaagtcgcca
    49321 aagaaatcca ttactatttc gacattgacg atatcagtat cgtcttacgc agccaccgta
    49381 aaaacaaact caacatctac tccactcact atcttgataa acaacatccc gcccacgaac
    49441 agagcgaagt cgatgaagcc ggaaccctca ccgaacgcgt gttcaaaagt aaagagatgc
    49501 tgctgatcaa tctccacgag cgggacgatt tagcccccta tgaacgcatg ttgttcgaca
    49561 cctggggcaa ccagattcaa accttgtgcc tgttaccgct gatgtctggc gacaccatgc
    49621 tgggcgtgct gaaactggcg caatgcgaag agaaagtgtt taccactacc aatctgaatt
    49681 tgctgcgcca gattgccgaa cgtgtggcaa tcgctgtcga taacgccctc gcctatcagg
    49741 aaatccatcg tctgaaagaa cggctggttg atgaaaacct cgccctgacc gagcagctca
    49801 acaatgttga tagtgaattt ggcgagatta ttggccgcag cgaagccatg tacagcgtgc
    49861 ttaaacaagt tgaaatggtg gcgcaaagtg acagtaccgt gctgatcctc ggtgaaactg
    49921 gcacgggtaa agagctgatt gcccgcgcga tccataatct cagtgggcgt aataatcgcc
    49981 gcatggtcaa aatgaactgc gcggcgatgc ctgccggatt gctggaaagc gatctgtttg
    50041 gtcatgagcg tggggctttt accggtgcca gcgcccagcg cattggtcgt tttgaactgg
    50101 cggataaaag ctccctgttc ctcgacgaag tgggcgatat gccactggag ttacagccga
    50161 agttgctgcg tgtattgcag gaacaggagt ttgaacgtct cggcagcaac aaaatcattc
    50221 agacggacgt acgtttaatt gccgcgacta accgcgatct gaaaaaaatg gtcgccgacc
    50281 gagagttccg tagcgatctc tattaccgcc tgaacgtatt cccgattcac ctgccgccac
    50341 tacgcgagcg tccggaagat attccgctgc tggcgaaagc ctttaccttc aaaattgccc
    50401 gtcgtctggg gcgcaatatc gacagcattc cagccgagac gctgcgcacc ttgagcaaca
    50461 tggagtggcc gggtaacgta cgcgaactgg aaaacgtcat tgagcgcgca gtattgctaa
    50521 cacgcggcaa cgtgctgcag ctgtcattgc cagatattgc tttaccggag cctgaaacgc
    50581 cgcctgccgc aacggttgtc gctcaggagg gcgaagatga atatcagttg attgttcgcg
    50641 tgctgaaaga aaccaacggc gtggttgccg ggcctaaagg cgctgcgcaa cgtctggggc
    50701 tgaaacgcac gaccctgctg tcacggatga agcggctggg aattgataaa tcggcattga
    50761 tttaactgca aattgccgga cagatctgcc tgtccggcat actattcacg aggttttttc
    50821 ggacgatatt tttccggcag ttctggcacc ggacgcttgt catcgatgag atgacgcacg
    50881 gttaagatcg gatgacgcca cagcattctc ggcccggccc aacgcataat ctgtttcatc
    50941 tcttcacgct ttgcaggctg gtaacagtgc accggacact gcttacaggc tggtttctct
    51001 tcgccgaaca cacatttatc cagccgcttt tgcgcgtaaa caaacaacgc ctcgtaatgc
    51061 tccggctccg ctgacgcctg cgggcatttc gcttgataaa gatcgatcat ttttttaatc
    51121 gtcagttttt cacgagagat acgcttgccg gacatgctgc ctccatactc ttaaggtgta
    51181 tttatattac aacttaattt gaaaggggac tatggctctg aaggaggagg tttattcaac
    51241 cgacgcaatt ttgcgtactt gcttcataag catcacgcaa aagctgcaaa acagcatctt
    51301 tcccggaacc agcatcaaga actcgccgtt cgcttcttcc cctgaaatga ttaactccgg
    51361 tatcatgtgc gccttatgtg attacaacga aaataaaaac catcacaccc catttaatat
    51421 cagggaaccg gacataaccc catgagtgca atagaaaatt tcgacgccca tacgcccatg
    51481 atgcagcagt atctcaggct gaaagcccag catcccgaga tcctgctgtt ttaccggatg
    51541 ggtgattttt atgaactgtt ttatgacgac gcaaaacgcg cgtcgcaact gctggatatt
    51601 tcactgacca aacgcggtgc ttcggcggga gagccgatcc cgatggcggg gattccctac
    51661 catgcggtgg aaaactatct cgccaaactg gtgaatcagg gagagtccgt tgccatctgc
    51721 gaacaaattg gcgatccggc gaccagcaaa ggtccggttg agcgcaaagt tgtgcgtatc
    51781 gttacgccag gcaccatcag cgatgaagcc ctgttacagg agcgtcagga caacctgctg
    51841 gcggctatct ggcaggacag caaaggtttc ggctacgcga cgctggatat cagctccggg
    51901 cgttttcgcc tgagcgaacc ggccgaccgc gaaacgatgg cggcagagct gcaacgcact
    51961 aatccggcgg aactgctgta tgcagaagat ttcgccgaga tgtcgctgat tgaaggccgt
    52021 cgcggcctgc gccgtcgccc gctgtgggag tttgaaatcg acaccgcgcg ccagcagttg
    52081 aatctgcaat ttggcacccg cgatctggtc ggttttggcg tcgagaacgc gccgcgcgga
    52141 ctttgtgctg ccggttgtct gttgcagtat gcgaaagata cccaacgcac gaccctgccg
    52201 catattcgtt ctatcaccat ggaacgtgag caggacagca tcattatgga tgccgcgaca
    52261 cgtcgtaacc tggaaattac tcagaacctg gcgggcggtg cggaaaatac gctggcttct
    52321 gtgctcgact gcaccgtcac gccgatgggt agtcgtatgc tgaaacgctg gctgcatatg
    52381 ccagtgcgcg atacccgcgt gttgcttgag cgccagcaaa ctattggcgc attgcaggat
    52441 ttcaccgccg ggctacagcc ggtactgcgt caggtcggcg accttgaacg tattctggca
    52501 cgtctggctt tacgaactgc tcgcccacgc gatctggccc gtatgcgcca cgctttccag
    52561 caactgccgg agctgcgtgc acagttagaa actgtcgata gtgcaccggt acaggcgcta
    52621 cgtgagaaga tgggcgagtt tgccgagctg cgcgatctgc tggagcgagc aatcatcgac
    52681 acaccgccgg tgctggtacg cgacggtggt gttatcgcat cgggctataa cgaagagctg
    52741 gatgagtggc gcgcgctggc tgacggcgcg accgattatc tggagcgtct ggaagtccgc
    52801 gagcgtgaac gtaccggcct ggacacgctg aaagttggct ttaatgcggt gcacggctac
    52861 tacattcaaa tcagccgtgg gcaaagccat ctggcaccca tcaactacat gcgtcgccag
    52921 acgctgaaaa acgccgagcg ctacatcatt ccagagctaa aagagtacga agataaagtt
    52981 ctcacctcaa aaggcaaagc actggcactg gaaaaacagc tttatgaaga gctgttcgac
    53041 ctgctgttgc cgcatctgga agcgttgcaa cagagcgcga gcgcgctggc ggaactcgac
    53101 gtgctggtta acctggcgga acgggcctat accctgaact acacctgccc gaccttcatt
    53161 gataaaccgg gcattcgcat taccgaaggt cgccatccgg tagttgaaca agtactgaat
    53221 gagccattta tcgctaaccc gctgaatctg tcgccgcagc gccgtatgtt gattattacc
    53281 ggcccgaaca tgggcggtaa aagtacctat atgcgccaga ccgcactgat tgcgctgatg
    53341 gcctacattg gcagctatgt accggcacaa aaagtcgaga ttggacctat cgatcgcatc
    53401 tttacccgcg taggcgcggc agatgacctg gcttccgggc gctcaacctt tatggtggag
    53461 atgactgaaa ccgccaatat tttacataac gccaccgaat acagtctggt gttaatggat
    53521 gagatcgggc gtggaacgtc cacctacgat ggtctgtcgc tggcgtgggc gtgcgcagaa
    53581 aatctggcga ataagattaa ggcattgacg ctgtttgcca cccactattt cgagctgacc
    53641 cagctaccgg agaaaatgga agacgtcgcc aacgtgcatc tcgatgcgct ggagcacgga
    53701 gacaccattg cctttatgca tagcgtgcag gatggcgcgg cgagcaaaag ctacggcctg
    53761 gcggttgcag ctctggcagg cgtgccaaaa gaggtgatta agcgcgcacg gcaaaaactg
    53821 cgtgagctgg aaagcatttc gccgaacgcc gccgctacgc aagtggatgg tacgcaaatg
    53881 tctttgctgt cagtaccgga agaaacctcg ccagcggtcg aagctctgga aaacctggac
    53941 ccggattcac tgactccgcg tcaggcgctg gaatggattt atcgcttgaa gagcctggtg
    54001 taataacaat tcccgatagt cttttgctat cgggaatatt aacgacaact gacgaataaa
    54061 ataaaaaccc cctgtataat aggaaagctt attttacagg gtaaaaccat gccatctaca
    54121 cgctatcaaa aaattaatgc ccatcactat cgccatatat gggtcgttgg tgatatacat
    54181 ggtgaatatc aattattaca atcccgctta catcaactct ctttttgccc tgaaaccgac
    54241 ttacttattt ctgtcggcga taatattgat cgtgggccgg agagtcttga cgtcctgcgc
    54301 ctgctaaacc agccctggtt tatctccgtt aaaggcaacc acgaagcaat ggagctggat
    54361 gcgttcgcga ccggcgatgg caatatgtgg cttgccagcg gtggtgactg gtttttcgat
    54421 ttaaatgatt cagagcaaca agaggcaata gatctgttgc tgaaattcca tcaccttcca
    54481 catattattg aaatcactaa cgacataata aaatatgtca tcgcacatgc agattatccg
    54541 ggtgatgaat atctctttgg caaagaaata gcggagagcg aattactctg gcctgtttat
    54601 cgtgtgcaga aatcgcttaa tggcgagtta caacaaataa acggcgctga ttattttata
    54661 tttggacata tgatgtttga taacattcag acgttcgcta accagattta tattgatacc
    54721 ggatcgccga aaagtgggcg gctgtcattt tataaaataa ggtagtctca tgcttcttct
    54781 gtgaagcatg agtaaccaga tgttattgca ggccattatt catttttcgc taccagcaaa
    54841 gagagatcct gcttcgccag cgcgcgactg gcactctccg gcaaaccgtc gtctgtaata
    54901 atctgatcaa actcgcttaa tggtaacgcc agccatgtcg ccacctgacc atatttcgtc
    54961 gcatcacaga ccaaaactcg ctggcggctg gcactagcaa tcgcccgttt caccgtgact
    55021 ttatcttccg ctggcgtaga aatcccccgc acactccatg acgatgcaga aataaaagcc
    55081 tgatcaatca tcaggctgcg cagcatggtc gcagcggctt ccccgacaca ggaacggttt
    55141 tcccgacaca ctgcaccgcc agtgtgaata attgtgcaat tactgttgtc gagcaagtag
    55201 ttcgcaataa cgaaatcgtt tgtgaccaca gtcagtgact ccatgtgaat cagatgctgt
    55261 gctatcgcta acgtggtcgt tcctgcatcc agatagatac aacttcccgg ctgaacaaga
    55321 cttgccgcca gcttgccaat agccgctttt tgcgtcattg ctagcgcagt ttttacctga
    55381 tgagaaggtt catgcgccac gcgtcccgga gactggacgc ctccggacac cagcacaacg
    55441 gctccctgct gctccagttt ttgtaaatcc cgacgaatgg tcatatgtga tacattcatt
    55501 ctgtccgtta gttcagcaat actgacaatg cctttttcag ctaccatctc aaggatgatt
    55561 tggcgacgct ctacgggtat caacttttgc tccttccttt gtcctgctga cattctacgc
    55621 tatttgcctg cgaaacgtgc gcggcgcaac taacgcttag ttcacataaa ataacacaca
    55681 atgttaattt atgtgaatcg gctcaccata tcgttatctt ccagcgctta tattcacaat
    55741 atcaaacaaa atatcactta aattaacaag gagagcagat gaaaacggga tctgagtttc
    55801 atgtcggtat cgttggctta gggtcaatgg gaatgggagc agcactgtca tgtgtccgcg
    55861 caggtctttc tacctggggc gcagacctga acagcaatgc ctgcgctacg ttgaaagagg
    55921 caggtgcttg cggggtttct gataacgccg cgacgtttgc cgaaaaactg gacgctctgc
    55981 tggtgctggt ggtcaatgcg gcccaggtta aacaggtgct gtttggtgaa acaggcgttg
    56041 cacaacatct gaaacccggt acggcagtaa tggtttcttc cactatcgct agtgctgatg
    56101 cgcaagaaat tgctaccgct ctggctggat tcgatctgga aatgctggat gcgccagttt
    56161 ctggtgatgc agtaaaagcc gctaacggtg aaatgactgt catggcctcc ggtagcgata
    56221 ttgcctttga acgactggca cccgtgctgg aagccgttgc cggaaaagtt tatcgcatag
    56281 gtgcagaacc gggactaggt tcgaccgtaa aaattattca ccagttgtta gcgggcgtac
    56341 atattgctgc cggagccgaa gcgatggcac ttgcagcccg tgcggggatc ccgctggatg
    56401 tgatgtatga cgtcgtgacc aatgccgccg gaaattcctg gatgttcgaa aaccggatgc
    56461 gtcatgtggt ggatggcgat tacaccccgc attcagccgt cgatattttt gttaaggatc
    56521 ttggtctggt tgccgataca gccaaagccc tgcacttccc gctgccattg gcctcaacag
    56581 cattgaatat gttcaccagc gccagtaacg caggttacgg gaaagaagac gatagcgcag
    56641 ttatcaagat tttctctggc atcactctac cgggagcgaa atcatgatca agattggcgt
    56701 tatcgccgat gattttaccg gcgcgacgga tatcgccagt tttctggtgg aaaacggtct
    56761 accaacggta caaattaacg gtgttccaac aggtaaaatg ccggaagcaa tcgacgcact
    56821 ggtgatcagc ctgaaaacgc gctcctgtcc ggtggttgaa gccacacagc aatcgctggc
    56881 ggctctgagc tggttgcaac agcaaggttg caaacagatc tatttcaaat actgctctac
    56941 tttcgacagt acggcgaaag gtaatatcgg cccggttacc gatgcattaa tggatgctct
    57001 cgacacgccg tttacggtct tctctccggc cctgtcggtc aacggacgta cggtttatca
    57061 ggggtatttg ttcgtaatga atcaactgct ggccgaatcc gggatgcgcc atcacccggt
    57121 aaatcccatg accgacagct atcttccccg tctggttgaa gcgcaatcca cagggcgctg
    57181 cggcgtcgtt tcggcacatg ttttcgaaca aggtgtggat gccgttcgtc aagagcaggc
    57241 tcgcttacag caagagggct accgctacgc ggtgcttgat gcgctgaccg aacaccatct
    57301 ggaaattcag ggagaagcct tgcgcgatgc cccactggta acgggcggtt ctggtctggc
    57361 gattggcctg gcccggcagt gggcgcaaga aaacggtaac caggctcgcg aagcagggca
    57421 tccgctcgct gggcgcggcg tagtgctctc cggttcatgc tctcaaatga ccaaccgcca
    57481 ggtggcacat taccgtcaaa ttgcaccagc ccgtgaagtt gatgtggcac gctgcctctc
    57541 aaccgaaact ctggccgctt atgcacacga actggcagag tgggttctgg gccaggaaag
    57601 tgtacttgct ccactggttt ttgccaccgc cagcactgac gcattggcag caattcaaca
    57661 gcaatacggt gcacaaaaag ccagtcaggc agtagaaact ctgttttctc aactagcggc
    57721 gcggttagca gcggaaggcg tgacacgctt tattgtcgca ggcggtgaga cctccggcgt
    57781 agtcacacag agcctgggca taaaagggtt tcatattggc ccaaccattt cccccggcgt
    57841 gccgtgggta aacgcactgg ataagcctgt ctcactcgcc cttaaatctg gcaacttcgg
    57901 tgatgaagcc ttttttcacg agcccaaaga gagtttttat catgagcgat ttcgcaaaag
    57961 tagagcagtc tttgcgagag gagatgacgc ggattgccag ttcattcttt cagcgcggct
    58021 atgcaaccgg ttcggctggc aatctgtcgc tgcttttacc tgacgggaat ttactggcga
    58081 caccgacagg ttcatgcctg ggcaatctcg atccgcagcg gctttccaaa gtcgccgcgg
    58141 atggcgaatg gttaagtggt gacaaaccct cgaaagaggt gctctttcat ctggcgctgt
    58201 atcgcaacaa tccgcgctgt aaagcggtgg tgcatttgca cagcacatgg tcgacggcgc
    58261 tttcctgcct gcaagggctg gacagcagca acgttattcg tccgttcaca ccatacgtgg
    58321 tgatgcggat gggaaatgtc ccgctggtgc cctattaccg accgggcgat aaacgcatcg
    58381 cacaggatct ggcggaactg gcagcagaca atcaggcttt tttactggca aatcatggcc
    58441 cagtggtttg cggtgaaagc ctgcaagaag ccgccaacaa tatggaagag ctggaggaaa
    58501 cggcaaagct gatttttatt ctcggtgacc gcccgatccg tatctgaccg caggtgaaat
    58561 tgcggaatta aggagttaat gcaatgcctc gttttgcagc taatttatcc atgatgttca
    58621 ccgaagtgcc ttttattgaa cgcttcgccg cagcgcgaaa agccgggttc gatgctatgg
    58681 aatttctgtt tccctatgac tactccaccc tgcaaatcca aaagcaactg gagcaaaacc
    58741 atctgacact ggcgctgttc aataccgctc ccggagatat taatgcgggg gaatggggat
    58801 tatccgccct tcccggacgt gagcacgaag cacacgcaga catcgacctg gcactggaat
    58861 atgcattggc gctcaattgt gaacaagtcc atgtgatggc aggcgtcgtg cccgctggcg
    58921 aagatgctga acggtaccgg gcagtattta tcgataatct ccgctacgct gccgaccgct
    58981 ttgcgccaca cggcaagcga atattagttg aagcactcag ccccgacgtt aagcctcatt
    59041 atctcttctc cagccagtat caggcactgg ctattgttga agaggttgcg cgagataacg
    59101 tgtttattca actagatact tttcatgcgc aaaaagtaga tggcaacctg acgcatttaa
    59161 tccgcgacta cgctgggaaa tatgcgcacg tacaaattgc cggactacct gatcgacatg
    59221 aaccggacga tggagaaatc aactatccgt ggctgttccg cctgttcgat gaggtgggat
    59281 atcagggctg gatcggttgt gaatataaac ctcgtggcct caccgaagaa gggcttggct
    59341 ggtttgacgc ctggcgctaa ttcgttaaat ccctaattac aacgtaccca tacatccccc
    59401 catctacgga tggggtaggt taactataac tttcagacag ggtttcccat gtccacaatt
    59461 acattgttat gcattgcgtt agctggcgta atcatgctgt tgctgctggt catcaaggca
    59521 aaggtacaac cattcgttgc tctgctcctc gtcagcctgt tagtcgcact tgcggcgggt
    59581 ataccggcgg gcgaagtggg taaagtgatg atcgccggga tgggcggtgt gcttggttcc
    59641 gtcactatta ttattggtct gggcgctatg ctggggcgta tgatcgaaca ctctggtggt
    59701 gcagagtcac tggctaatta tttcagtcgc aagttaggtg acaaacgaac tatcgctgcg
    59761 ctgactctgg cagcgttctt cctcggtatt cccgtcttct ttgatgtcag ctttattatt
    59821 cttgcgccaa tcatttacgg ttttgccaag gttgccaaaa tatcgccact caaatttggc
    59881 ctgcctgtcg ctggaatcat gctaactgtt catgtggcag taccgccgca tccaggccct
    59941 gtcgccacag cggggttact ccacgcagac atcggctggc taaccatcat cggtattgcg
    60001 atttctattc ccgtagggat tgttggctac tttgcagcga aaataatcaa taagcgccaa
    60061 tatgcgatgt cagtagaagt actggaacag atgcaactgg ctccggccag tgaggaaggc
    60121 gcgacaaaat taagcgataa aataaatcca ccgggcgtcg cgctggtcac ctcgctaatt
    60181 gttattccta tcgcgattat catgacgggt acggtttccg caacactgat gccgccttcg
    60241 catcccctgc ttggtacgct acagctgatc ggctcaccaa tggtagctct aatgattgcg
    60301 ctggtgctgg cattctggtt attggcttta cgtcgcggct ggagcttaca acacaccagc
    60361 gacattatgg gctcagccct tcccactgcg gcggtagtga ttttggttac tggtgctgga
    60421 ggggtatttg gcaaagtgct ggtggaatcg ggcgtcggca aagcccttgc caatatgcta
    60481 caaatgattg acctgcctct gttaccagcc gcatttatta tttcactggc tctgcgtgca
    60541 tcgcaggggt cagcaaccgt agcaatcctg acaaccggcg ggttactctc agaagcggtg
    60601 atgggattga atccgattca gtgcgtattg gtgacgctgg cagcctgctt tggtgggctt
    60661 ggcgcgtcac atattaatga ctcagggttc tggattgtga ccaaatatct ggggttgtcg
    60721 gtagcagacg gtctgaaaac ctggacagtg ttaacgacca ttctcggttt taccggattc
    60781 ttaattacct ggtgcgtatg ggcggtaatt tgaccttaat aaaaaggtcc gatgggcatc
    60841 ggacctttta ttgtgcacag aaaaggccag cctcgcttga gactggcctt tctgacagat
    60901 gcttacttac tcgcggaaca gcgcttcgat attcagcccc tgcgtttgca ggatttcacg
    60961 caaacggcgc aggccttcaa cctgaatctg gcgaacacgt tcacgggtga ggccaatttc
    61021 acgacctaca tcttccagtg ttgccgcttc gtaccccagc aaaccgaatc gacgtgccag
    61081 cacttcacgc tgtttggcgt tcagctcgaa cagccatttg acgatgctct gcttcatatc
    61141 gtcatcttgc gtggtatctt ccggaccgtt ctctttttca tcggccagga tgtccagcaa
    61201 cgctttttcg gaatcaccac ccagcggggt gtctaccgag gtaatgcgct cgttaagacg
    61261 aagcatacgg ctgacgtcat caactggctt atccagttgc tctgcgatct cttccgcact
    61321 tggttcatgg tccagcttat gggacaactc acgtgcggtt cgcaggtaaa cgttcagctc
    61381 ctttacgatg tgaatcggca aacgaatagt acgggtttgg ttcataatcg cccgttcaat
    61441 cgtctggcga atccaccagg ttgcgtatgt tgagaagcgg aaaccacgtt ccgggtcaaa
    61501 cttctctacc gcgcggatta gccccaggtt gccctcttcg ataaggtcca gcaacgccag
    61561 accacgattg ccataacggc gggcaatttt taccaccaga cgcaagttac tctcgatcat
    61621 ccggcggcga gaggcgacat ctccacgcag tgcgcgacgc gcaaaataaa cttcttcttc
    61681 ggccgttaac agtggtgaat aaccaatctc accaaggtaa agctgagtcg cgtccaacac
    61741 tcgctgtgtg gctccctgcg ataacagttc ctcttcggcc aaatcgttat cactgggttc
    61801 ctcttctact aaggcctttt cgtcaaaaac ctcaactccg ttctcatcaa attccgcatc
    61861 ttcatttaaa tcatgaactt tcagcgtatt ctgactcata aggtggctcc tacccgtgat
    61921 cccttgacgg aacattcaag caaaagcctg gttccgccga tttatcgctg cggcaaataa
    61981 cgcagcgggt ttacggattt ccccttgtaa cgaatttcaa aatgcaagcg tgttgaactg
    62041 gttccggtgc tacccatggt tgctattttt tgccccgcct tcacttcttg ttgttcccgg
    62101 accagcattg tgtcgttatg ggcgtaggca ctcaggtaat catcattatg tttgatgata
    62161 atcagattac cgtagccgcg cagcgcgtta ccggcataaa caacgcggcc atctgcggtc
    62221 gcgataattg cctgtccttt gctgcctgcg atatcaatcc ccttgttgcc cccctcagaa
    62281 gcgccaaagg tttcgatcac tttgccctca gtcggccagc gccaggtgga gataggcgta
    62341 ctggttgatg tactgctgac agttggctcg gttgtgcttg ctgttggtac cgttacaggc
    62401 gctgtgaccg tggtcgcagc tggcttgttg ttcggcaaca ttttgttagc actctgttca
    62461 cccgaagatt cagaatacgt aattgtcggt tgcgacgcaa cagcaacggt ggaattttgt
    62521 gcagtcttga tcacaactcc ttgctctgct gcgtcggcct gggtaatggc atttccgcca
    62581 gtgattggcg taccggaagc attacccacc tgcaaggtct gaccaacgtt cagcgcgtat
    62641 ggtgcctgaa tattgttgcg ctgagcaagg tcacggaaat cgttgccagt aatccaggcg
    62701 atatagaaaa gtgtgtcgcc ttttttcacg gtataggtac tgccgctata actgcctttc
    62761 ggaatgttcc catactgacg gttatagacg atgcgtccgt tttccatctg taccggctgc
    62821 tgagctactg gctgcactgg ctggatttgc ggttgctggg ctgtagacgt cgtccccatt
    62881 ttcggcggcg gcgtaatcaa cataccagaa ttggtatttg caggcgcatt gccattaacg
    62941 gagctgaccg gtgccggtgg atttgaagtg tcagaacagc ctgccagcca tagcgaaacc
    63001 agtgacaaag ccgcaatgcg gcgaacggtg aattttgggc ttcccgcgct catttatccc
    63061 ccaggaaaaa ttggttaata accagtgaca taattaccgt gcaaggcacc ctactgaaca
    63121 ctggaaaaga tgttcacgat acgctgacct gcggcaaaat aaccaggaaa aatccaggta
    63181 tttcctcacg ttttaagcca gctccccctt cactaaaggg acaaagcgca cggcctccac
    63241 ggtatcgata ataaattcgc ctccccgacg acgcacccgt ttcaaatact ggtgctcctc
    63301 ccctacgggt aagacgagaa tcccgccttc gtccagctgc gtcattagcg cagttggaat
    63361 ttccggcggt gccgccgtaa caatgatagc gtcaaacggc gcgcgtgcct gccaaccttg
    63421 ccatccatcg ccatgacggg ttgaaacatt atgtaaatca agatttttca ggcggcgacg
    63481 cgcctgccac tgcaaacctt taatccgttc gaccgagcaa acatgctgga caagatgcgc
    63541 caggattgcc gtttgatatc ccgaaccggt gccaatttcc agcacccgcg actgcggcgt
    63601 cagctcgagt aattcggtca ttcgcgccac catatacggc tgcgaaattg tctgcccctg
    63661 acctatcggc aaggcgatat tgtcccaggc tttttgttca aacgcttcat caacgaattt
    63721 ttcacgcggc acggcagcaa gtgcattcag cacctgctca tcctgaatac cttgcgcacg
    63781 taattgatcc agaagtgctt gtacgcgtct gcttaccatt gcgtgccaac tcccacgctg
    63841 tttaaccagt ctgaaaccac atcttgcgcg ctatgcgcag ttaaatccac atgcagcggc
    63901 gtgatggaga catagccctc atctaccgca gcaaaatcgg tccccggacc ggcatcacat
    63961 ttaccgcccg gcgggccaat ccagtacagc gtattgccgc gcggatcttg ctgcgggatc
    64021 acctgatctg ccggatgtcg tgtaccgcag cgcgtcacgc gaataccttt gatttgatcc
    64081 aagggtaaat ccggaacgtt aatattaaga atacgtccgg tgcgcagcgg ctctttacac
    64141 agtgcgcgca aaattgaaca ggttaccgcc gcggcagtgt cgtaatgttt atgcccgtca
    64201 agcgagacgg ccagcgccgg aaaacctaaa tgacggcctt ccatcgcggc ggctaccgta
    64261 ccggaataaa taacgtcatc ccccagattc ggcccggcgt taatgccgga cacaacaatg
    64321 tccgggcgcg gacgcatcag agcattcacg ccaagataga cgcaatcggt cggggttccc
    64381 atttgcacag caatatcacc attttcaaag gtaaacgtgc gcagggagga ttccagtgtc
    64441 agagaatttg aagcgccgct gcggttacga tcgggggcga ccacctgaac gtcagcaaac
    64501 tcacgcaagg ctttcgccag cgtttgtata ccgggtgcat gtaccccgtc atcattactc
    64561 agcaatatgc gcataatcac ctgttgtgtt gataagttcc ctgacaacgc tggttgcaaa
    64621 actacccgcc ggaagccaga aacggatctc tacggtgacg tcatcccacc aattccagct
    64681 taattgttgc ggatacagca gcatcgctct gcgcgcggct tcaacttttt cgcgcaccag
    64741 taaagcttgt aattcagttt ctgcggcgac agctgcttgt tcgaatgcca gcgctttacg
    64801 ctgagttccc cattcgccac tgcccggcaa tgcggcggtt atcatcagct ctttatcgtt
    64861 gacgcgatgc tgtaattccg ccagttcttc ggtggttgct acaaaccagc taccacgtcc
    64921 ggctaattgt agcgcatcgc cgtcaacaac ttgattaacg tctgcttttt tgaggcgctc
    64981 agcaacaatc tgattaaaca acgcactgcg ggctgccgac aaccaaaaac tccgtttatt
    65041 gcgatcgcgc accggagtat tggtttgcgc ccagcgcagc gcgccctgca agttgctacc
    65101 gccaatccca aaacgttggg caccgaagta gttcggtaca ccttttacgc aaatatcgat
    65161 cagacgttgt tcaacgtcat cgcgattgct cacttcgcgc agaaccaggg taaaggcgtt
    65221 acctttcagc gcgcctaaac gcagcttgcg cttgtgccgc gcatactcca gcacctggca
    65281 gccttccagt tgaaaggcgc tcagatcggg catttccttg cctggcacgc gagcgcataa
    65341 ccactgttcc gtaacagcat gtttgtcttt ttgcccagcg aagctgactt cacgggcatg
    65401 aattttcagg aatttcgcca gtgcatccgc cacaaaacgg gtattgcagc cgtttttgag
    65461 gattctaacc agaatatgct caccttcacc atcaggctca aagcccaaat cttccaccac
    65521 cacaaagtct tccggattgg ctttcagcag cccggtgcct tgcggtttac cgtggaggta
    65581 agtgagatta tcaaactcaa tcattttgtt gccttaatga gtagcgccac cgcttcacag
    65641 gcaatccctt ccccacgtcc ggtaaatcca agtttttccg tagtagtggc tttcacgtta
    65701 acatcatcca tatggcagcc gagatcttcg gcaataaaca cgcgcatttg tggaatgtgc
    65761 ggcaacatct tcggtgcctg agcgatgata gtgacatcga cgttgcccag ggtataaccc
    65821 ttcgcctgaa tacgacgcca ggcttcgcgt agcagctcgc ggctgtcggc acctttaaat
    65881 gccggatcgg tatccgggaa cagcttgccg atatccccca gcgccgccgc gccaagcaat
    65941 gcatcggtca acgcatggag cgccacgtcg ccatcagaat gcgccagcaa tcctttttcg
    66001 taaggaatgc gtacgccacc aatgataatt gggccttcac cgccaaaggc atgtacgtca
    66061 aaaccgtgtc caattcgcat tatgtattct cctgatggat ggttcgggtg aggtaaaact
    66121 cggccagtgc caaatcttcc gggcgcgtga ctttaatgtt atccgcacgg ccttcgacca
    66181 actgaggatg gaatccgcaa tattccagcg ccgaggcttc gtcggtaata gtcgcgcctt
    66241 catttagagc gcgcgtcaga cagtcatgta acagctcacg agggaaaaat tgcggcgtca
    66301 gcgcgtgcca taagccgttg cgatcaacgg tatgagcaat ggcatttttg cccggttcgg
    66361 cacgtttcat cgtatcgcgc actggtgcgg ctaggatccc tcccgtgcgg ctggtttcgc
    66421 tcaacgccaa caatcgcgcg aggtcatcct gatgcagaca aggacgagcg gcgtcatgca
    66481 ccaataccca ctgcgcgtcg ccagcggctt tcaaacctgc cagcacggaa tcggcacgct
    66541 catcaccgcc atctacaacg gtgatttgcg gatgattcgc cagaggaagt tgtgcaaaac
    66601 ggctatcgcc aggacttatg gcaatgacga cacgtttcac ccggggatgc gccagcagcg
    66661 catgcaccga gtgttcaaga atggtttgat taccgattga gagatattgc ttaggacatt
    66721 ccgtttgcat tcgacggcca aatccggccg ccggaaccac ggcgcaaaca tccaaatgag
    66781 tggttgccat gttaattccc gggctgattt atcgattgtt ttgccccgca gactgtgcgc
    66841 gcttcgacgc gtcaggcacc agacgataaa aagtttcgcc cggcctggtc atgctgagtt
    66901 cattacgcgc acgctcttcg agcgcctcct ggccgccatt gagatcgtca atttcggcaa
    66961 aaagttgatc gtttcgcgct ttaagtttcg cgtttgtagc ttgctgtgcc gccacatcat
    67021 cattgacgcg ggtatagtca tgtataccgt tcttaccgaa ccacagcgaa tactgtagcc
    67081 agaccagaat agccagcaac agcagcgtta gtttacccat cctgccccct gaaaaacggc
    67141 atcatcatcc catgcatccg aagacgactc tacatcctct gttggggata ccgcgacaac
    67201 gcgggcaaat gtaccacatt tgtccattgt tacgtatacc tagggcgtgc agaacataat
    67261 ctcattatta gttacggttt gaattatgaa cagaggagac aagaaagtac aaattagccc
    67321 agtagccaca taaacagtgc gccaaacata atgcctactg tcaccagggt gaaaacaata
    67381 ctgtagcgta gctttccgtc catcaatgaa tgcagcgcaa tccccaccac taccgcgaca
    67441 ggcatcagtg ccagaaagaa aggccaggtg tagataaaga agaacagcgt gttagagcca
    67501 taaatcaaca tcggcatcgc cagcgcaaat aaccaggaga taaaaccgac cacggcacca
    67561 ggtagtgacc atgtggtttc ttcatcctca gtaaggctgt cgttatttgt tagtgtaatg
    67621 ttatggctat tacgcatatt tgatcctgtt actttgacga accgggcatg gaaacccggt
    67681 ggtgtctcag gatctgataa tatcgttctg tctcaacaga tctaataatt gctgtaccaa
    67741 atttgttact aattgttcac cattgagatg aatttctgcc gattcaggcg cttcgtaaac
    67801 ggaatctatt cccgtaaagt tgcgcagttc accggcacgc gctttcttat ataagccttt
    67861 gggatcgcgg gcttcgcaaa tcgccagcgg cgtatcgaca aacacttcga taaagcgccc
    67921 ttctcctacg cgttcgcgaa ccatctggcg ttcggcgcgg tgtggcgaga taaatgcggt
    67981 cagcaccacc agtccggctt caaccatcaa attcgccact tcaccgacgc gacggatatt
    68041 ctctttacga tcggcatcgc taaaaccgag atcgctgcat aatccgtggc gaacattgtc
    68101 gccatccagc agatacgtac tgacgccgag tttatgtaac gcctcctcca gcgccccggc
    68161 gaccgttgat ttaccggacc cagagaggcc ggtaaaccac agcactacac cacgatgacc
    68221 gtggtgtagc tcgcgttgtt gcacagtgac cggatggcta tgccagacga cgttttcgtc
    68281 atgcagcgcc attatttatc ccccagcaaa tcgcgcgcgc cccagtgcgg aaagtggcga
    68341 cgaaccaggg cattcaattc cagttcgaat gcactgaatt cagatgacgc aacggcggcc
    68401 tcgtttttcg gctcatgcac cataccggca ccaacagtca cgttgctcag gcgatcgata
    68461 aaaatcagcc cacccgtcac cggattttgt tgataacgat ctaacaccag cggctcgtca
    68521 aaagtgagat ccacgaggcc gatcccattc agtggcaggt tttcaacttc acgctgggta
    68581 aggttattaa tatcaaccgg atagcgaatg ccatcaacac gcgcgcgcgt cttcttaccg
    68641 gcaattttga tgtcgtaact ctggcccggg gaaagcggct gttccgccat ccataccaca
    68701 tccaccgacg cgctcggcac cgccggtaac gcttcgtctg ccgccagcag cagatcgcca
    68761 cggctgatgt cgatctcatc cgtcagcacc agggtgatcg cttctccggc aaaggcttct
    68821 tcgcgatcac catcaaaagt cacgatccgc gcgacgtttg attccacacc agagggcagc
    68881 gatttgacac gttgcccgac ttccacgcga ccggatgcca gcgttccggc gtagccacga
    68941 aaatcgagat tcgggcgatt aacgtactgc accgggaagc gcattggctg ggcatccacc
    69001 actcgctgga tctccacggt ttccagcact tcgagcagtg tcggaccgct gtaccacgac
    69061 atactttcac tttgcgatgc cacgttgtcg ccttccagtg cagagagcgg cacaaagcgg
    69121 atatccagat tacccggcag ctgcccggca aaggtcaaat aatcttcacg aatatgggtg
    69181 aacgtctctt cactgtaatc caccagatcc attttgttga tcgccacgac cagatgtttg
    69241 atccccaaca gtgtggagat aaaactgtga cgacgggttt gatcgagcac gcctttacgg
    69301 gcatcgatca gtaagatcgc cagttcacat gtcgatgcgc cagttgccat attgcgagtg
    69361 tactgctcgt gccctggggt gtcggcgata ataaatttac gcttctcggt agagaaataa
    69421 cggtaagcca cgtcaatggt gatgccctgt tcgcgctcag cttgcaggcc gtccaccagc
    69481 agtgccagat ccagcttttc gccctgggtg ccgtgacgct tactgtcgtt atgcagcgat
    69541 gagagctgat cttcgtagat ttggcgggta tcgtgcagca ggcgaccaat cagggtactt
    69601 ttgccgtcat cgacgctacc acaggtcaga aaacgcagca ggcttttatg ttgttgcgca
    69661 atcatccagg cttcgacgcc gccttcattg gcgatttgtt gtgcaagtgc ggtgttcatc
    69721 ttaaaaatac ccctgacgtt ttttcagctc catagacccc gcctggtcgc ggtcaatcac
    69781 gcggccctga cgttcactgg tggtagaaac cagcatctct tcgatgatct ccggcagtgt
    69841 ttgtgcattt gactccaccg caccggtcag cggccagcag cccagcgtac ggaaacgcac
    69901 catccgtttt ttaatcactt cgcccggttg caggtcgata cggttgtcat caatcatcat
    69961 caacataccg tcgcgttcca gaaccggacg ttccgcagcg agatacagtg gcacaatgtc
    70021 gatattttcc agccagatgt attgccagat atcctgctcg gtccagttag agagcgggaa
    70081 gacgcggatg ctttcacctt tgttaatttg cccgttgtag ttgtgccaca gctccgggcg
    70141 ctgatttttc ggatcccagc gatggaagcg gtcacggaaa gagtagattc gctctttagc
    70201 gcgggatttc tcttcatcac ggcgcgcacc accgaaggcg gcatcaaaac cgtatttgtt
    70261 cagcgcctgt ttcaggcctt cagttttcat aatatcggta tgtttcgcgc tgccgtgtac
    70321 gaatggatta atccccatcg ccacgccttc cgggttttta tgcaccagca gctcgcagcc
    70381 gtaggcttta gcagtacgat cgcggaactc atacatctcg cggaatttcc agccggtatc
    70441 gacatgcagc aacgggaaag gcagcgtacc tggataaaac gccttgcgcg ccagatgcag
    70501 catgacgctg gaatctttac cgatagagta gagcatcacc ggatttgaga attctgccgc
    70561 cacctcgcga ataatgtgga tgctttccgc ctccagttgc cgcaggtgag taagtcgttt
    70621 ttgagccata accgttcctt tgcaataccg ctattttctt gccatcagat gtttcgacta
    70681 tagggagcgt aagagaacga atgaaattac caattagaat gagtagttcc ttaacggaat
    70741 aacgatttgg caaagctaat atcaaaaagt gcttaaggca ccggatttcg ggcgtttagg
    70801 aagatttgaa attgttttag cgcagcggca gtttcatact atggcggtaa aaaaatttgc
    70861 atggtattta aggactcact atgttttccg cattgcgcca ccgtaccgct gccctggcgc
    70921 tcggcgtatg ctttattctc cccgtacacg cctcgtcacc taaacctggc gattttgcta
    70981 atactcaggc acgacatatt gctactttct ttccgggacg catgaccgga actcctgcag
    71041 aaatgttatc tgccgattat attcgccaac agtttcagca aatgggttat cgcagtgata
    71101 ttcggacatt taatagtcgg tatatttata ccgcccgcga taatcgtaag agctggcata
    71161 acgtgacggg aagtacggtg attgccgctc atgaaggcaa agcgccgcag cagatcatca
    71221 ttatggcgca tctggatact tacgccccgc tgagcgatgc tgacgccgat gccaatctcg
    71281 gcgggctgac gttacaagga atggatgata acgccgcagg tttaggtgtc atgctggaat
    71341 tggcagaacg cctgaaaaat acgcctaccg agtatggtat tcgatttgtg gcgaccagcg
    71401 gcgaagagga agggaaatta ggcgctgaga atttactcaa gcggatgagt gacaccgaaa
    71461 agaaaaatac gctgctggtg attaatctcg ataacttaat tgttggcgat aaattgtatt
    71521 tcaacagcgg tgtaaaaacc cctgaagcag taaggaaatt aacgcgcgac agggcgctgg
    71581 caattgcgcg cagtcacgga atagccgcaa cgaccaatcc ggggttgaat aaaaattatc
    71641 cgaaaggcac tgggtgttgt aatgacgcag aaatattcga caaagcgggc attgctgtac
    71701 tttcggtgga agcgactaac tggaatcttg ggaataagga tggttatcag caacgcgcaa
    71761 aaacagccgc attccctgcg ggaaatagct ggcatgacgt aagactggat aatcaacagc
    71821 atattgataa ggcacttcct ggaagaatag aacgtcgctg ccgtgacgtt atgcggataa
    71881 tgctaccgtt ggtgaaggag ctggcgaagg cgtcttgatg ggtttgaaaa tgggagctgg
    71941 gagttctacc gcaggggcgg ggaattctaa gtgatatcca ttatcgcatc cagtgcgccc
    72001 ggtttatccc cgctggcgcg gggaacacac taagcataca taactgtttt taaacaaatt
    72061 tattccgcat caacaatcta ccaactaaat taaaacattt ccttattttt aaagaacgga
    72121 taacttattg attatcaaca ggaagaaaag aaaccaaacg taacccatcc aaatccaccg
    72181 gaatacgtct gttttctccc caggtctgaa attcaaaacc cgactcggta ttggtcgccc
    72241 aggccatcac cacatttccg caaccagcca gttgggtaat ttgctgccag atcatctccc
    72301 gaatacgttt tgatgtatca ccaacataca caccggcacg cacttccagt agccagattg
    72361 cgagccgtcc acgtaagcgt ggcgggacat tttctgtaac aaccacgacc atgctcatca
    72421 gccgcgtcct cgatgaccgc tatcgcccaa cgtttcaggt tcggggatgg ctggcggtaa
    72481 catatccggc gcaggttgtg gcggttcaat ttcacctgcg gcaaggactt cctcaattaa
    72541 cggtattaat ttgcccgtta acttagtgct acggaaaata tcacgacagg ctaatctgac
    72601 ttctttatca ggttctgcgg gttgcctcgc cgctatttca aatgcctttg gtacaaccga
    72661 atcaaattta atgatatcgg ctatgtcata aacaaatgaa agcggtttgc cactatggat
    72721 aaacccaata gcgggcgcat atcccgcggc taatactgcc gcttcagata taccgtacag
    72781 acatgatgta gcagcactga tgcagcgatt cacaacatcg cctttttccc agtctttagg
    72841 atcgtatttg cgaccattcc atttcacacc atattgtttc gccagtaatg catacgtctg
    72901 gcgaacgcga gatccctcaa ttccacgtag ctgctccact gaacggcgag ctggcggtgg
    72961 ctcacgaaaa cgtaattcat acattttgcg caccaccttc agacgtagat cttccgttaa
    73021 agccagcttt gcctggtaga gtaacttatc agcacgcgcc ccaccgggtt gtccggaaga
    73081 gtagacgcga acacccgctt caccgaccca gacctgtagc gtccccaccg tggcggccag
    73141 atgcaccgcc gcgtgggaaa ctctcgttcc cggttcgagc ataatgcagg cgaccgatcc
    73201 caccggaatg tgcgtgcgga tcccggtttt atcgatcagc acgaaagcgc cgtccagtac
    73261 gtcgatttgc ccgtactgga ggaagatcat agaggtgcga tcttttaacg ggatcgggct
    73321 cagcggtaca aacgtcacgc ctctgctccg ggtttgatca gcatcagacc acagccgaac
    73381 gcgcggcttt tgccataccc ctgactgaga cgttgcagaa ataacccggg atcggtgacc
    73441 gtaagcatcc ccgtataatc cacgctactg aactggatca gttgccggga gttttcccgc
    73501 cgcagttgct gttgtcggta ggcatcaacc gagatatcca gcagtgtaaa accacttctc
    73561 tccccctgcg ctgccagcca gtccagcgcc gcctgttgtt gatgcaacca gacatcactt
    73621 ccttccgcct gccccctcac ctgccgtttc gcctccatca gcagatcgtg gcgcttgccc
    73681 gctttacaga ttgttggatt cgcccgcagg ttgaaacaaa gttgttgtcc ggtacacagc
    73741 tcgggcgcaa atgaccggca ttctatggtg aacgtatcac tttccgccgg gcgttcctgc
    73801 gacagtacaa aaaagcgaaa cgcgccctgg agttcttccc gacgataaag aaattgcctt
    73861 tctttgccgc tagggaagag atcccacagc cactgatgca tcacatattc accgcgatcc
    73921 accagatgca gcaactgcgc aggcgaaagc tggccggtat gtaaggttat tcttgagagg
    73981 tacatggttc ctccttgctg agccacggcc cctgattgac ggtgcgttcc ccaaacagcc
    74041 actgctgacg atttaaagga acatcccggc ggcgtaatat tttgctcgcg accaggccgt
    74101 cgtgtttccc ttcccaccag cattcatcct gaagtttcgg gagtgaaact ttcagtttat
    74161 ggaaatgatc ctgatactgt tggtatgcat tacataaggc atcacacgcg ttgccttcga
    74221 gcagtaacgg cgcaagaggt aatgccagcg gatgactttt tcgccccaga taaagcggaa
    74281 aaactggatg gcgtaaaccg tcctgcaact gttcgaggct gtaaggcgca tcggctgttg
    74341 ttgccaccgc caccatccac caggcatcgg tgtagtagtc gcgccgggag ataatcgcgc
    74401 tcaaaagctc agggtcgctc aactcttcgc gacggctgaa ataacgtgct ttacgcacct
    74461 cttttggcat ctggaccgtg tgataatccc gtgcccagcg cgggttacgg ctggcgcaaa
    74521 ccaccagtga atagtggcga ttaaacgcgt ttaagcgttc ggtatcatca cgccggatcc
    74581 ctaccccggc agccagcagc cccagcaatg ctgagagcga aggcagttca tgggtatgac
    74641 gcacttcgcc gggggcatcg acgccccagg atgccatcgg cccatgaagc tgaaaaatca
    74701 gatattggct cataagccgc cccttacgcg cagataaagt ccagcacgtc cttcatgctt
    74761 ccctgtttgt tcatcacgtc aaaacttgcg taatcggtct tctgttcgta gaccgtattc
    74821 atattttcgc gaagagttgt aatacgctgc accgccacct ctaactgacg ggtgccatta
    74881 atgggttcat agaaagctgc cgccagagaa cgcggttgat cggtgccttt ttctgccagc
    74941 gcccaggagg cataagcccg gctggcaaag ctgttttgtt taccggttgg ggagacttta
    75001 agcgcggctt ccgtaaaggc gcgtatggtc tgattagcta acgcttcgtc accgccgagg
    75061 ttttctacca gcagatcttt atcaatgcag ataagatata ggtgtagaac agcgcagaac
    75121 cgaatccggt ttcatctaca tgaccggcac cggcatcttc agaagcctgg cgtaaatcat
    75181 ctacggcggt gaaaaaatca ttttcgacaa tcgtttcact gacaccaaac gcatgcgcga
    75241 cctggcaggc ggcttctaca ttaaacccgg gttttttcgc cagcatacga ccaaacatag
    75301 cgatatctac cgccatacgg tctttacgta acaaagcgag atctttctct tttggcgcgc
    75361 gcttttcttc tgccagttga tgggccagcg cttttacggc gtcaaattct gccgggctga
    75421 tatggacgag ctgttcagtt tcggcgctag tgagcggatc ttttggtttt ttgtcgtttt
    75481 tttgtttttt gtcttttttg gctttcccaa gataatcggc tatctctact gcccattcga
    75541 tggctttttt atcttcgatt cctttctcaa tcaggatagt tgccgcctca cgcgcaatac
    75601 gcccgctacg aacaccaata tggcccgcca gcgcctgttc aaaaagttca gaagtgcgcc
    75661 aagcacgctt cagactttgc gaggaaacgc gcagacgcgt tgctccaccc agaatcacag
    75721 ttttcggaga gccggaatca tcacggttaa gattggcagc agggtaagcg gttaacaaat
    75781 gaagctgaat aaatgttgtc atgagatagt cctttattaa ttttgttcgt tatcggcgtc
    75841 gccagcctgg taatattcca gcgcccagcg cccagcgccc agcgcccagc gcccagcgcc
    75901 cagcggatac ggataaattc tgttggtcgc tgctggcggc ggtgatgatt gagcaggtca
    75961 tcgctctcct ggcaccagcg gaagaccccc tcttctagag agtcaagatt gacggaaccg
    76021 tttaataatt tgaccgcacg gcgtaactgc cgcaataaat catccggcgt ttttactgcc
    76081 aacaaacgag taaaccgccc ctttttcatt accgccgcca gttgcgcggc aaaaggctgc
    76141 cgttcgtcaa tagctttaac attggcgcta actgctgcta tcagcgctag tgcggtgata
    76201 cgccactcgg gttcatcctg ccactttatt tgtctgttct taagaaacag gcgaaatcca
    76261 tccgtcagac aaacatcatt aaccgtcgta ctacgccgca gactggcacg ctcgccgcgt
    76321 ttatcctgca actcttcatg ccatttacgc agcgcaactt tatgctcatc ttttacaata
    76381 ctcattcagc agcctcctgc tttttctccc tggcggcgtt agcattttgc ttctccgctg
    76441 atgttgtaaa atatttcttg cgtgcggtca tgacgcgttc caaatcaacg ggctcatatg
    76501 gattggtaaa gacacgatcg tcaaaatcct gacgcgcgaa taaccacatt tccttttgcc
    76561 atttactgag taattcgtcc ggatcctgac cttcttcaat tttgcgcact aatctcagga
    76621 atcgatgctg agttttgttc cagaagtcaa tatccacaaa accgaaatca agctttgtgg
    76681 ttttcttctc agagaaccat gcttctttca acgctcggtg taacaagctt agaatgtgtg
    76741 atgccgcttg cgctgccaga cgcaacttag gtattaggtc ttcttttttg tttaacaaca
    76801 gcgggaaatg gtgttcatac caacaacgaa ccttcatatc ttcaaaatca aaaccaaatc
    76861 cccataagcc cacccttgtc tgctttagat tgctggcatt caaaagtttc acgacctgtg
    76921 cggggagttc agtattgttt tttgagttac ctacctctat gaggcctaac cagtcgcgcc
    76981 agattaaacc accaggttgt ggtttaactg agtaaaaatc tccaccctct ttaagcggca
    77041 gacggtaagg tgttagagga tggcgccaca tgacgtattg aacaccatag tttttgagtg
    77101 acatcaagcc caaaagagca tcgctctgcc taccgcaaat gtcgcagtta cctattgagg
    77161 ttgtcttaaa atcaatacgt atacgtcgtg gcattcccca gtaggcctga agtttattga
    77221 cttgttcatc cgttaccaca gcaccgtcca gttcgctggt acgcgtcggt gcaagccaag
    77281 gaaagaccag gtcatcaaag gtttttggta aaggtaaatc ggcttcatct tgcggcatta
    77341 cgtttagcca cagctttcgc cacaaagggg tttgttgatt gccctgatac tccagcaatt
    77401 ctataagtgt cgtcagaggc cctccgccac gtagcccagg atagtaaccg cgtccaccta
    77461 taggtgcatt caactggatt gaaaaaagcg ctaaaggcac acaatgcagg cacagatgct
    77521 gagttgtgtc tcgcttaata aaatggtctg tatttcgttc ttttgtttgt tttcctggtg
    77581 cgtcaggaag aagcgaagca ataaaagttg ccttaacttt taattcatca aaatcctgca
    77641 taaatgaagg ggaatctggc ccaaactgaa aagcatgttc taaagacagc aatgcttccc
    77701 ttagcttttc agcttccagt ccgtcttccc agatatcatc ccaactacgt tgatcttttg
    77761 gcgcgaagct ggtttgtagt agccccagca aaaactgcca ggccgccccc tggagatctg
    77821 cccgcggcgc agcgatatcg acaacatttt catcagccag atcgactggt gccagtttgc
    77881 ctgtcgttcc gtctttaaaa cgaacgggca accacggggt tgtcagaagt gaaaacgagt
    77941 tcatacgcat cctctcgtaa aaaagaccat cccaggtcgg ttgtccatcc ttccatcctt
    78001 ttctgtatat caactattca gagagtcttg tcgcagacat ctcaaccaac cttgccaatc
    78061 aatccctccc tggccgaata cccgcaactt tcgtcatcgg tgactaaaat cacgtttgcc
    78121 atttccggat cttgccgctg ttcaacgcac cactgcctga acgcatcccc ttccagtaaa
    78181 gaaaactcat cccgatgttt tttccaccag cttcggcgca ctctgacaac gctcatttcc
    78241 catgcgtgag caccggtggc ataaggcttc accacaccgt caatacaggt agccagccac
    78301 agggaaacag actcttcagc cagacgtgtc gacagttttt ccggaaggta atcgttgata
    78361 ttggcggcat agccgagcct gaagttcagc acatactttt tagcccttgc gcgatcacag
    78421 taatatttgc ccacttgctc ctgctcgctg cgggcaaatc cttccggcat taccacgtcc
    78481 tcaccgtaaa ctgattcaat aagaagccgg gctgcgtgcg gcatctgaat agcgccttgc
    78541 tcacgcagca cacgctgcgt cagccagatt cgcccatgat cgggatagac atatgcactg
    78601 ttacgcatgg cactgccgaa ccattcgtca ccaggagcgt catcccagac gggggccaga
    78661 atcagcaatt caggggggga acgctcgtct tttccgtcac gctttaactg accattaata
    78721 tcgcggatat gccgctgtaa tcgccccgct cgctggatta gcaaatcaat gggggccagg
    78781 tcggagatca tttcgtccag gtcaatatcc acgctttgtt caattacttg tgtacaaaca
    78841 atgacttgag acgctctctg taaactacaa tatttaccaa agcgtgccag cgtttccgtt
    78901 tcaattcgct ggcgatcgct aaaagcaaag cggctatgaa aaagtgaaag gctggaagca
    78961 ggaatgacgc cgcgggcaag cagctgacga tacacctgaa tagcgtcatc aacagaattc
    79021 cgaatccagg cgatgcattt cccctgactt accgccgatt cgatacgcgc aatacactct
    79081 tgttcactat ggagccaacc cacgctcacg ctacgttcaa cttctttgcg cgtcgctacc
    79141 cggtgcgaat gtacatcggt tttcgtgaca tgcgtcagcc aggggtaatc atcttttccc
    79201 aggagtgggg cttcttgctg gccctctgca ccacgcgcaa aggcggcgac gagtttgtcg
    79261 cgctgctgtt gagataacgt agcagaaagc aaaataacgc tgtttccgcc acgcgcctgc
    79321 cgctcgatca gcccttcaag aatgcacgac atataagcgt cacaggcgtg gatctcatca
    79381 gccagcagga ttttgttact cagccccaga agccgcagat tattatgttt aaacggcatc
    79441 actgccatca tcgcctgatc cagggtgccg acgccaattt cagccagcaa cgccttcttg
    79501 ttactgtcgg caaactaggc cgcacaaccc tggctgaaag cttgttcatc cggttcttct
    79561 gacccgatta aatcaccgga ccagagtgat tcat
//
LOCUS       DAJVUJ010000003        77358 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.003, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000003 DAJVUJ010000000
VERSION     DAJVUJ010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 77358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 77358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..77358
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.003"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..1624
                     /locus_tag="OQQ48_000184"
     CDS             <1..1624
                     /locus_tag="OQQ48_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCS1951078.1"
                     /translation="NNGSQVNVTDSSLVSDTIGLTNGSSLNIGANGVVATDHLTVDSY
                     STVNLTESTGWNNYSNLYTNTITVTNGGVLDVNVDQFDTEAFRTDKLELTSGNIADHN
                     GNVISGVFDINSSDYVLNADLVNDRTWDTSKSNYGYGIVAMNSDGHLTINGNGDVDNG
                     TELDNSSVDNVVAATGNYKVRIDNATGAGTIADYKDKELIRVNDANTNATFSAANKAD
                     LGAYTYQAEQRGNTVVLQQMELTDYANMALSIPSANTNIWNLEQDTVGTRLTNSRHGL
                     ADNGGAWVSYFGGNFNGDNGTINYDQDVNGIMVGVDTKIDGNNAKWIVGAAAGFAKGD
                     MNDRSGQVDQDSQTAYIYSSAHFANNVFVDGSLSYSHFNNDLSATMSNGTYVDGSTNS
                     DAWGFGLKAGYDFKLGDAGYETPYGSVSGLFQSGDDYQLSNDMKVDGQSYDSMRYELG
                     VDAGYTFTYSEDQALTPYFKLAYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSF
                     TKNFSAYTDANYLGGGDVDQDWSANVGVKYTW"
     gene            1712..2335
                     /gene="iprA"
                     /locus_tag="OQQ48_000185"
     CDS             1712..2335
                     /gene="iprA"
                     /locus_tag="OQQ48_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HCS1951079.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNSEDT
                     FFILEGVISLRREENVLIGITQASYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINCLPSEY"
     gene            complement(2336..3493)
                     /gene="ampH"
                     /locus_tag="OQQ48_000186"
     CDS             complement(2336..3493)
                     /gene="ampH"
                     /locus_tag="OQQ48_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HCS1951080.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            3845..5065
                     /gene="sbmA"
                     /locus_tag="OQQ48_000187"
     CDS             3845..5065
                     /gene="sbmA"
                     /locus_tag="OQQ48_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HCS1951081.1"
                     /translation="MFKSFFPKPGAFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLVA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            5078..6172
                     /gene="yaiW"
                     /locus_tag="OQQ48_000188"
     CDS             5078..6172
                     /gene="yaiW"
                     /locus_tag="OQQ48_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HCS1951082.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(6231..6539)
                     /locus_tag="OQQ48_000189"
     CDS             complement(6231..6539)
                     /locus_tag="OQQ48_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HCS1951083.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            6799..7011
                     /locus_tag="OQQ48_000190"
     CDS             6799..7011
                     /locus_tag="OQQ48_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HCS1951084.1"
                     /translation="MNLPVKIRRDWHYYAFAIGVIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            complement(7035..8129)
                     /gene="ddlA"
                     /locus_tag="OQQ48_000191"
     CDS             complement(7035..8129)
                     /gene="ddlA"
                     /locus_tag="OQQ48_000191"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCS1951085.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPSKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLRLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            8207..8364
                     /locus_tag="OQQ48_000192"
                     /pseudo
     CDS             8207..8364
                     /locus_tag="OQQ48_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8591..8851
                     /gene="iraP"
                     /locus_tag="OQQ48_000193"
     CDS             8591..8851
                     /gene="iraP"
                     /locus_tag="OQQ48_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HCS1951086.1"
                     /translation="MKNLIAELLLKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIEQVEGALYEVKPDASIPDDDTELLRNYVKKLLKHPRQ"
     gene            8952..10367
                     /gene="phoA"
                     /locus_tag="OQQ48_000194"
     CDS             8952..10367
                     /gene="phoA"
                     /locus_tag="OQQ48_000194"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HCS1951087.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNAVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            10486..10806
                     /gene="psiF"
                     /locus_tag="OQQ48_000195"
     CDS             10486..10806
                     /gene="psiF"
                     /locus_tag="OQQ48_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HCS1951088.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            10908..12023
                     /gene="adrA"
                     /locus_tag="OQQ48_000196"
                     /gene_synonym="dgcC"
     CDS             10908..12023
                     /gene="adrA"
                     /locus_tag="OQQ48_000196"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HCS1951089.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(12040..12849)
                     /gene="proC"
                     /locus_tag="OQQ48_000197"
     CDS             complement(12040..12849)
                     /gene="proC"
                     /locus_tag="OQQ48_000197"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCS1951090.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPNKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            12969..13427
                     /locus_tag="OQQ48_000198"
     CDS             12969..13427
                     /locus_tag="OQQ48_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCS1951091.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            13610..14134
                     /gene="aroL"
                     /locus_tag="OQQ48_000199"
     CDS             13610..14134
                     /gene="aroL"
                     /locus_tag="OQQ48_000199"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HCS1951092.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            14184..14375
                     /gene="yaiA"
                     /locus_tag="OQQ48_000200"
     CDS             14184..14375
                     /gene="yaiA"
                     /locus_tag="OQQ48_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HCS1951093.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            14633..15310
                     /locus_tag="OQQ48_000201"
     CDS             14633..15310
                     /locus_tag="OQQ48_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HCS1951094.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            15382..15666
                     /gene="ppnP"
                     /locus_tag="OQQ48_000202"
     CDS             15382..15666
                     /gene="ppnP"
                     /locus_tag="OQQ48_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HCS1951095.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            15814..16155
                     /locus_tag="OQQ48_000203"
                     /pseudo
     CDS             15814..16155
                     /locus_tag="OQQ48_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            16152..16235
                     /locus_tag="OQQ48_000204"
     CDS             16152..16235
                     /locus_tag="OQQ48_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1951096.1"
                     /translation="MTQRPWSKLQHKTHNIAALKIIVRRSE"
     gene            complement(16313..17224)
                     /gene="rdgC"
                     /locus_tag="OQQ48_000205"
     CDS             complement(16313..17224)
                     /gene="rdgC"
                     /locus_tag="OQQ48_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HCS1951097.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            17349..18257
                     /gene="mak"
                     /locus_tag="OQQ48_000206"
     CDS             17349..18257
                     /gene="mak"
                     /locus_tag="OQQ48_000206"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HCS1951098.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHISGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFAMDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVPQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            complement(18400..19584)
                     /gene="araJ"
                     /locus_tag="OQQ48_000207"
     CDS             complement(18400..19584)
                     /gene="araJ"
                     /locus_tag="OQQ48_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HCS1951099.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMFFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADTPVLAKP
                     LG"
     gene            complement(19710..22847)
                     /gene="sbcC"
                     /locus_tag="OQQ48_000208"
     CDS             complement(19710..22847)
                     /gene="sbcC"
                     /locus_tag="OQQ48_000208"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HCS1951100.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLLTAQQQEQQSLNWLTRLDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHTRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NGSLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVKAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHKRQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTTLQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALSDLVSHKT
                     RIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVKKI
                     NGLGYSKLESTFAVK"
     gene            complement(22844..24046)
                     /gene="sbcD"
                     /locus_tag="OQQ48_000209"
     CDS             complement(22844..24046)
                     /gene="sbcD"
                     /locus_tag="OQQ48_000209"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HCS1951101.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALAESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            24236..24925
                     /gene="phoB"
                     /locus_tag="OQQ48_000210"
     CDS             24236..24925
                     /gene="phoB"
                     /locus_tag="OQQ48_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HCS1951102.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            24983..26278
                     /gene="phoR"
                     /locus_tag="OQQ48_000211"
     CDS             24983..26278
                     /gene="phoR"
                     /locus_tag="OQQ48_000211"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HCS1951103.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            26685..28004
                     /gene="brnQ"
                     /locus_tag="OQQ48_000212"
     CDS             26685..28004
                     /gene="brnQ"
                     /locus_tag="OQQ48_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HCS1951104.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            28080..29453
                     /gene="proY"
                     /locus_tag="OQQ48_000213"
     CDS             28080..29453
                     /gene="proY"
                     /locus_tag="OQQ48_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HCS1951105.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            29609..31331
                     /gene="malZ"
                     /locus_tag="OQQ48_000214"
                     /pseudo
     CDS             29609..31331
                     /gene="malZ"
                     /locus_tag="OQQ48_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
     gene            complement(31336..31917)
                     /gene="acpH"
                     /locus_tag="OQQ48_000215"
     CDS             complement(31336..31917)
                     /gene="acpH"
                     /locus_tag="OQQ48_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HCS1951106.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRRETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFI
                     CYARKQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLNAHYTALGTRFWQFYPRMMAQASHKAL"
     gene            32010..33080
                     /gene="queA"
                     /locus_tag="OQQ48_000216"
     CDS             32010..33080
                     /gene="queA"
                     /locus_tag="OQQ48_000216"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCS1951107.1"
                     /translation="MRVTDFFFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDDRSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            33136..34263
                     /gene="tgt"
                     /locus_tag="OQQ48_000217"
     CDS             33136..34263
                     /gene="tgt"
                     /locus_tag="OQQ48_000217"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCS1951108.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            34286..34618
                     /gene="yajC"
                     /locus_tag="OQQ48_000218"
     CDS             34286..34618
                     /gene="yajC"
                     /locus_tag="OQQ48_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCS1951109.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            34646..36493
                     /gene="secD"
                     /locus_tag="OQQ48_000219"
     CDS             34646..36493
                     /gene="secD"
                     /locus_tag="OQQ48_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HCS1951110.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            36504..37475
                     /gene="secF"
                     /locus_tag="OQQ48_000220"
     CDS             36504..37475
                     /gene="secF"
                     /locus_tag="OQQ48_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HCS1951111.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLTQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            37604..37951
                     /locus_tag="OQQ48_000221"
     CDS             37604..37951
                     /locus_tag="OQQ48_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HCS1951112.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(38128..39012)
                     /gene="tsx"
                     /locus_tag="OQQ48_000222"
     CDS             complement(38128..39012)
                     /gene="tsx"
                     /locus_tag="OQQ48_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HCS1951113.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLNFGPFKEWYFANNYIYDMGRNKNGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(39311..39850)
                     /locus_tag="OQQ48_000223"
     CDS             complement(39311..39850)
                     /locus_tag="OQQ48_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCS1951114.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGGYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            40001..40450
                     /gene="nrdR"
                     /locus_tag="OQQ48_000224"
     CDS             40001..40450
                     /gene="nrdR"
                     /locus_tag="OQQ48_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HCS1951115.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            40454..41557
                     /gene="ribD"
                     /locus_tag="OQQ48_000225"
     CDS             40454..41557
                     /gene="ribD"
                     /locus_tag="OQQ48_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HCS1951116.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVASMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRLRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHHIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWGEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            41646..42116
                     /gene="ribE"
                     /locus_tag="OQQ48_000226"
     CDS             41646..42116
                     /gene="ribE"
                     /locus_tag="OQQ48_000226"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HCS1951117.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            42136..42555
                     /gene="nusB"
                     /locus_tag="OQQ48_000227"
     CDS             42136..42555
                     /gene="nusB"
                     /locus_tag="OQQ48_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCS1951118.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            42633..43610
                     /gene="thiL"
                     /locus_tag="OQQ48_000228"
     CDS             42633..43610
                     /gene="thiL"
                     /locus_tag="OQQ48_000228"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HCS1951119.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGVRIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTLDWKGYDHFATP"
     gene            43588..44106
                     /gene="pgpA"
                     /locus_tag="OQQ48_000229"
     CDS             43588..44106
                     /gene="pgpA"
                     /locus_tag="OQQ48_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HCS1951120.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(44160..45134)
                     /gene="yajO"
                     /locus_tag="OQQ48_000230"
     CDS             complement(44160..45134)
                     /gene="yajO"
                     /locus_tag="OQQ48_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HCS1951121.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHGWAQFVSMQDHYNLIYREEEREMLPLFYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHPVVGFK"
     gene            complement(45189..47051)
                     /gene="dxs"
                     /locus_tag="OQQ48_000231"
     CDS             complement(45189..47051)
                     /gene="dxs"
                     /locus_tag="OQQ48_000231"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HCS1951122.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            complement(47076..47975)
                     /gene="ispA"
                     /locus_tag="OQQ48_000232"
     CDS             complement(47076..47975)
                     /gene="ispA"
                     /locus_tag="OQQ48_000232"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HCS1951123.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSPDTSALEALADYIIQRNK"
     gene            complement(47975..48217)
                     /gene="xseB"
                     /locus_tag="OQQ48_000233"
     CDS             complement(47975..48217)
                     /gene="xseB"
                     /locus_tag="OQQ48_000233"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCS1951124.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            48423..49871
                     /gene="thiI"
                     /locus_tag="OQQ48_000234"
     CDS             48423..49871
                     /gene="thiI"
                     /locus_tag="OQQ48_000234"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCS1951125.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPEVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIALQTE
                     QEVVEVETVNGFGPNDVILDIRSIDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            complement(49925..50515)
                     /gene="yajL"
                     /locus_tag="OQQ48_000235"
     CDS             complement(49925..50515)
                     /gene="yajL"
                     /locus_tag="OQQ48_000235"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HCS1951126.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLQVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(50478..51389)
                     /gene="panE"
                     /locus_tag="OQQ48_000236"
     CDS             complement(50478..51389)
                     /gene="panE"
                     /locus_tag="OQQ48_000236"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCS1951127.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSANLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            51557..52048
                     /gene="yajQ"
                     /locus_tag="OQQ48_000237"
     CDS             51557..52048
                     /gene="yajQ"
                     /locus_tag="OQQ48_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HCS1951128.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            complement(52176..53540)
                     /locus_tag="OQQ48_000238"
     CDS             complement(52176..53540)
                     /locus_tag="OQQ48_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1951129.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHILLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPA
                     YVSSLRIEIPANIAANEALKVRLLETEGIKEVLIAEEEHSAYVKIDSKVTNRFDVEQA
                     IRQA"
     gene            complement(53689..54579)
                     /gene="cyoE"
                     /locus_tag="OQQ48_000239"
     CDS             complement(53689..54579)
                     /gene="cyoE"
                     /locus_tag="OQQ48_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HCS1951130.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            complement(54591..54920)
                     /locus_tag="OQQ48_000240"
     CDS             complement(54591..54920)
                     /locus_tag="OQQ48_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HCS1951131.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            complement(54920..55534)
                     /locus_tag="OQQ48_000241"
     CDS             complement(54920..55534)
                     /locus_tag="OQQ48_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HCS1951132.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTCLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            complement(55524..57515)
                     /gene="cyoB"
                     /locus_tag="OQQ48_000242"
     CDS             complement(55524..57515)
                     /gene="cyoB"
                     /locus_tag="OQQ48_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HCS1951133.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWANSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            complement(57537..58484)
                     /gene="cyoA"
                     /locus_tag="OQQ48_000243"
     CDS             complement(57537..58484)
                     /gene="cyoA"
                     /locus_tag="OQQ48_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HCS1951134.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            complement(58945..60420)
                     /gene="ampG"
                     /locus_tag="OQQ48_000244"
     CDS             complement(58945..60420)
                     /gene="ampG"
                     /locus_tag="OQQ48_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HCS1951135.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            complement(60464..61042)
                     /locus_tag="OQQ48_000245"
     CDS             complement(60464..61042)
                     /locus_tag="OQQ48_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCS1951136.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLAASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKITKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            61347..61664
                     /gene="bolA"
                     /locus_tag="OQQ48_000246"
     CDS             61347..61664
                     /gene="bolA"
                     /locus_tag="OQQ48_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HCS1951137.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            62008..63306
                     /gene="tig"
                     /locus_tag="OQQ48_000247"
     CDS             62008..63306
                     /gene="tig"
                     /locus_tag="OQQ48_000247"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCS1951138.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDSAVEA
                     EDRVTIDFTGSVDSEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            63551..64174
                     /gene="clpP"
                     /locus_tag="OQQ48_000248"
     CDS             63551..64174
                     /gene="clpP"
                     /locus_tag="OQQ48_000248"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCS1951139.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            64300..65574
                     /gene="clpX"
                     /locus_tag="OQQ48_000249"
     CDS             64300..65574
                     /gene="clpX"
                     /locus_tag="OQQ48_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HCS1951140.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            65762..68116
                     /gene="lon"
                     /locus_tag="OQQ48_000250"
     CDS             65762..68116
                     /gene="lon"
                     /locus_tag="OQQ48_000250"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HCS1951141.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENCVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            68325..68597
                     /gene="hupB"
                     /locus_tag="OQQ48_000251"
     CDS             68325..68597
                     /gene="hupB"
                     /locus_tag="OQQ48_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HCS1951142.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            68789..70660
                     /gene="ppiD"
                     /locus_tag="OQQ48_000252"
     CDS             68789..70660
                     /gene="ppiD"
                     /locus_tag="OQQ48_000252"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCS1951143.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            70811..71182
                     /locus_tag="OQQ48_000253"
     CDS             70811..71182
                     /locus_tag="OQQ48_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HCS1951144.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            71276..71674
                     /gene="fadM"
                     /locus_tag="OQQ48_000254"
     CDS             71276..71674
                     /gene="fadM"
                     /locus_tag="OQQ48_000254"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HCS1951145.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(71726..72421)
                     /gene="queC"
                     /locus_tag="OQQ48_000255"
     CDS             complement(71726..72421)
                     /gene="queC"
                     /locus_tag="OQQ48_000255"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HCS1951146.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(72486..74186)
                     /locus_tag="OQQ48_000256"
     CDS             complement(72486..74186)
                     /locus_tag="OQQ48_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HCS1951147.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            74286..75068
                     /gene="cof"
                     /locus_tag="OQQ48_000257"
     CDS             74286..75068
                     /gene="cof"
                     /locus_tag="OQQ48_000257"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HCS1951148.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTH"
     gene            75257..75715
                     /gene="decR"
                     /locus_tag="OQQ48_000258"
     CDS             75257..75715
                     /gene="decR"
                     /locus_tag="OQQ48_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HCS1951149.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            75745..>77358
                     /locus_tag="OQQ48_000259"
     CDS             75745..>77358
                     /locus_tag="OQQ48_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCS1951150.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYFLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEAS"
BASE COUNT        18886 a        19169 c        20571 g        18732 t
ORIGIN      
        1 aaataatggc tctcaggtaa atgttactga ttcctctctg gtatcagata ccattggttt
       61 aaccaacggt tcttcactga atattggtgc aaatggcgtg gttgctacag accacctgac
      121 tgttgatagc tacagtaccg ttaatttaac cgaatctacc ggttggaata attattctaa
      181 tctgtatacc aatactatca ctgtgactaa cggtggtgtg ctggatgtga atgttgatca
      241 gttcgatact gaagctttcc gtactgataa actggaactg accagcggca acattgctga
      301 ccataatggc aacgtaattt ctggtgtgtt cgacatcaac agcagcgatt acgttctgaa
      361 cgctgatctg gtgaacgacc gtacgtggga tacttccaag tctaactacg gttacggtat
      421 tgttgctatg aactctgatg gtcacctgac tatcaatggt aacggcgacg tagacaacgg
      481 tactgaactg gataacagct ctgttgataa cgttgttgct gcaaccggta actacaaagt
      541 tcgtatcgac aacgcaactg gcgctggcac tatcgctgat tacaaagata aagaacttat
      601 tcgggtaaac gacgctaaca ccaacgcgac cttctctgct gctaacaaag ctgacctggg
      661 tgcatacacc tatcaggctg aacagcgcgg taacaccgtt gttctgcaac agatggagct
      721 gaccgactac gctaacatgg cgctgagcat cccgtctgcg aacaccaata tctggaacct
      781 ggaacaagac accgttggta ctcgtctgac caactctcgt catggcctgg ctgataacgg
      841 cggcgcatgg gtaagctact tcggtggtaa cttcaacggc gacaacggca ccatcaacta
      901 tgatcaggat gttaacggca tcatggtcgg tgttgatacc aaaattgacg gtaacaacgc
      961 taagtggatc gtcggtgcgg ctgcaggctt cgctaaaggt gacatgaatg accgttctgg
     1021 tcaggtggat caagacagcc agactgccta catctactct tctgctcact tcgcgaacaa
     1081 cgtctttgtt gatggtagct tgagctactc tcacttcaac aacgacctgt ctgcaaccat
     1141 gagcaacggt acttacgttg acggtagcac caactctgac gcgtggggct tcggtttgaa
     1201 agccggttac gacttcaaac tgggtgatgc tggttacgag actccttacg gcagcgtttc
     1261 tggtctgttc cagtctggtg atgactacca gctgagcaac gacatgaaag ttgacggtca
     1321 gtcttacgac agcatgcgtt atgaactggg tgtagatgca ggttatacct tcacctacag
     1381 cgaagatcag gctctgactc cgtacttcaa actggcttac gtctacgacg actcaaacaa
     1441 cgataacgat gtgaacggtg attccatcga taacggtact gaagggtctg cggtacgtgt
     1501 tggtctgggt actcagttca gcttcaccaa gaacttcagc gcctataccg atgctaacta
     1561 cctcggtggt ggtgacgtag atcaagactg gtccgcgaac gtgggtgtta aatatacttg
     1621 gtaatattct tcactccgaa gaaattctgg taatttaatc taaataatgc ccgtcaagga
     1681 tttgacgggc attactgcaa aggacgcgca aatgttatct gtagttaaac ctcttcagga
     1741 atttggtaag ctcgataaat gtttgtccag atacggtacg cgcttcgagt ttaataatga
     1801 aaagcaagtt atattttcca gtgatgtcaa tagcgaagat actttcttta ttttagaggg
     1861 cgttatctct ctgcgtagag aagaaaacgt acttatcggt attacccagg cttcttatat
     1921 tatggggctg gctgatggtt taatgaaaaa cgatatacca tacaaattaa tatcagaagg
     1981 aaattgtacg ggatatcatc taccagccaa acaaaccatt acgcttattg aacaaaatca
     2041 actctggcga gacgcttttt actggttagc ctggcaaaat agaattctgg aattacgcga
     2101 cgtgcagctc attgggcata attcctacga acaaatccgc gcaacattat tatcaatgat
     2161 tgactggaat gaagaattgc gatcacgtat tggtgtgatg aattatatcc atcaacgtac
     2221 acgcatatcg cgttctgtcg tcgcagaagt tctcgctgct ttgcgtaaag gcggctatat
     2281 cgaaatgaat aaaggcaaac tggtcgctat caactgtttg ccttcagagt attaatcagg
     2341 atgcagggat aaccaacggt ttattcccgc ttagctcggt caccagatca ttgatgccat
     2401 cgctcatatt tttaaagcgc gtcaacggcg aacgagtgac caccacaaac gcgccaatat
     2461 tcttctgcgg gatcatcgcc atataggtaa tgaaaccgcc accaccgcct gtcttctgaa
     2521 taatccccgg acgaccctct ttcggggcca tatataccca gcctaaacca agcgcatccg
     2581 ctttgccggg gacatccatg ccaatcactt tggtaaactg cgcgcgctga taaatcaacg
     2641 tctgcatgcg atctgcctgg ttactgcgct gataaaaatc agatgataaa tactgctgca
     2701 tccagcgcat catatcgcca ggcgtggaat agacgccgcc gctgccaatt gccgccagcg
     2761 tgttattgca cggactggca ccgcgctcag caaccattaa acggcggcac tgatccggtg
     2821 agggggtgta ggtggtgtct ttcatcccca atggacgggt aatctgctct tcaaacaact
     2881 gggtataagg cttgccggac gcattcgcca gtgcatctgc cagcaggtcg aacgcaaggt
     2941 tagagtacgc cgcctgactg cctggcgcgg ctttcagctt cgccgtagaa aggtatttcc
     3001 agcgttgctc gcgcgttggc cagacaaaca ccggacgatg tgccgcgcca ccgggctgtt
     3061 cacggggcag ggcgctggta tgggttgcca gattcactag ggtaatgggc gtcccattgt
     3121 aggttggcac gcgtgcgcct ggcggagcat atttacttag cggatcgttt aacttcacgg
     3181 tcccctggtc gagcaatttc accagcattt cactggtcat tagcttggtg agggaagcaa
     3241 tacgcacgac cgaatccagc tgcgggcgaa cgttattacc aggtcgcgtt tcgccataac
     3301 tgcgaaagac gcgctggtta ccgtcgataa caaccagcgc catccccgtg gcaccgctgc
     3361 cgtaaaaaat atgatcggca taacgatcga caatatcaga ggcaaactcc ggttcagtga
     3421 tcggctgcgc cgcatggacg gaggtcaatg acgccgcaca cagcacggca gaaaaaagca
     3481 gactacgttt caacggtggt gtccatagat tgaatgaagg taactgagcg tatttatact
     3541 actcattgat ggattgcaaa gggcgaatta gtgcaataat agtgttaaaa acgtaacggc
     3601 gcgtaaacac gatctttttc ctgctcacca atgcctgcat ttgtgatcct gcgcgtgtta
     3661 atccgcttat gtaaacaatc tttggtttgt gaaatccgtc gaaacaattc ttatggtcag
     3721 gctggcgaac taagcgcctt gctatgggtc acaatgggcg gtttcatttg gctgacgctt
     3781 tgtagtggtc atgcggttaa tacacagcag ttgttaaaac gataagaagt tagcaggagt
     3841 gcatatgttt aagtcttttt tcccaaagcc gggagcgttt tttctctcgg cctttgtttg
     3901 ggcattgatt gccgttatct tctggcaagc cggtggggga gactgggtgg cgcgtatcac
     3961 cggcgcttcc gggcagatcc cgattagcgc cgcgcgtttc tggtcgttgg atttcctgat
     4021 tttttacgct tactacattg tttgcgtagg actttttgca tttttctggt ttatctacag
     4081 cccgcaccgt tggcaatact ggtcaatact cggtactgca ctgatcatct tcgtcacctg
     4141 gtttttggtg gaagtcggag tcgccgtcaa cgcctggtat gcaccgttct atgatctgat
     4201 tcaaaccgcg ctaagttcgc cgcataaagt cactatcgaa caattttacc gcgaagtggg
     4261 cgtctttctg gggattgcgc tgatcgcggt ggtgatcagt gtactgaaca acttctttgt
     4321 cagtcactat gtgttccgct ggcgtacggc gatgaacgaa tattacatgg cgaactggca
     4381 acaactgcgt catatcgaag gtgccgcaca gcgtgtgcag gaagacacca tgcgttttgc
     4441 ttcaacgctg gagaatatgg gcgtcagttt tatcaacgcc atcatgacgt tggtcgcctt
     4501 cctgccggtg ctggtaacgc tctccgcgca tgtgccggag ctgccgatta tcgggcacat
     4561 tccgtatggt ctggtgattg ccgctatcgt ctggtcgctg atggggaccg gattgctggc
     4621 agtggtaggg atcaaactgc cggggctgga gtttaaaaac cagcgagtag aggccgccta
     4681 ccgtaaagag ctggtttatg gtgaagacga tgccacgcgc gcgacgccgc ctacggtacg
     4741 cgagctgttt agcgccgtac ggaaaaacta ttttcgcctc tattttcact atatgtattt
     4801 caacatcgcc cgcattctct atttgcaggt cgataacgtt ttcggtttgt tcttgctgtt
     4861 tccgtcaatt gttgccggta cgattacgct cggcctgatg acgcagatta ccaacgtttt
     4921 tggtcaggtt cgcggtgctt tccagtacct gattaactca tggaccacac tggttgagtt
     4981 gatgtctatc tacaaacgtc tgcgcagctt tgaacatgag ctggatggtg acaaaattca
     5041 ggaagtaacc cataccttga gctaaaaagg agaagtaatg tcgcgcgtca atcatctatc
     5101 ttcgctgtca ttgttggcgg ttctggtttt agcggggtgt agtagtcagg ctccgcaacc
     5161 gttgaaaaag ggcgaaaaag cgatagatgt agcgagcgtc gtgcggcaga agatgcctgc
     5221 gagcgtgaaa gaccgcgatg cctgggcgaa agatctcgcc accacctttg aaagtcaggg
     5281 gctggcaccg acgctggaaa acgtctgctc ggtgctggcg gtggcgcagc aggagtcaaa
     5341 ttatcaggca gatccggccg ttccgggctt aagcaagatc gcctggcaag agattgaccg
     5401 tcgtgctgaa cggatgcaca ttcctgcttt tctggttcat accgcgctaa aaatcaaatc
     5461 gccaaacggc aaaagttata gcgaacggtt agattcggtg cgtacagaaa agcaattgag
     5521 cgcgattttt gacgatctga tcagcatggt accaatgggg cagacgctgt ttggttcgct
     5581 taatccggtg cgcaccggtg ggccgatgca ggtcagcatt gcttttgccg aacagcatac
     5641 caaagggtat ccgtggaaaa tggacggtac agttcgtcag gaagtcttca gtcgccgcgg
     5701 cgggttgtgg tttggtactt accatttact gaattatccc gccagctata gcgcaccgat
     5761 ataccgcttt gctgatttta acgctggctg gtacgccagc cgtaatgccg cgtttcagaa
     5821 cgcggtcagt aaggccagcg gcgtgaagct ggcactggac ggtgatttaa ttcgttacga
     5881 cagtaaagaa cccgggaaaa ccgaactggc aacgcgcaaa ctggcgggaa aactggggat
     5941 gagcgacagc gaaattcgcc gtcagttaga gaaaggtgac agcttctctt ttgaggagac
     6001 ggcgctgtac aagaaagttt atcaacttgc cgaagcgaaa acgggtaaat cactaccccg
     6061 cgaaatgttg cctggcattc aactggaaag cccgaaaatc acccgcaacc tgactacggc
     6121 ctggtttgcg aagcgcgtag acgaacggcg ggcgcgttgt atgaaacagt gatcaaacag
     6181 gaatgtcatg ccagataagg cgttttaggc cgtatctggc aacgtaaatc ttactggcgg
     6241 cgatgtcgcc agtgcagcca tagcgccacc actgcaaaaa tgaggcagcc tactaaaaac
     6301 gggatcagcc cgaaaatggt gccgacacct aaaccaattt ccacccgtgg gcgacctgtt
     6361 tcctggacct gcattaaacg ctcatagacg ccgggcgcat gcactaacag gttcagcaat
     6421 tgcgccccag cccaaaagca aaacagcaca aacaacgcat aggcaatgtt gccaggcgtt
     6481 gaagaggcat tgcgatattt tccgctaaac agcgattttg acagggtgaa atcagccata
     6541 aaaacctccg gaagtcatct gcttctcttc tggtgtgata ccagcggctt atggcaagtc
     6601 tgacaggtca tttagattgt caatatcaga atcatggtaa attgatgttg ggaatattcc
     6661 cgaagcgtga atcttcgttg tgagtcacaa aatggcaacc ttaagttaaa tttattaaca
     6721 attacgaaaa tgttgtctac accacagacg gttgttacag aatatgcaag gatgttgttt
     6781 ttcgttaacg gagctgccat gaatctgcct gtaaaaatcc gccgtgactg gcactactat
     6841 gcgttcgcca ttggcgttat attcattctt aatggcgtgg tggggttact gggatttgaa
     6901 gcaaaaggtt ggcagaccta tgccgtcggt ctggtgacgt gggtgattag tttctggctg
     6961 gcggggttga ttattcgtcg tcgcgatgaa gaaactgaaa acgcccaata agaatattat
     7021 cgggcgttaa aatattacat tgtggttttc aatgcgttat ccgcagcgtg acgctccagc
     7081 gccagctcaa tcaaacgtgt gatcagatcg gtgtaaccca gaccgctggc ttgccacagc
     7141 ttcggataca tactgatgtt ggtgaagcca ggcagtgtgt tgatctcgtt gatcaccact
     7201 tcgttctctg gggttaaaaa cacgtctaca cgcgccatgc ctgcgcatcc caacgtttga
     7261 taagcctgaa cggcaatcgc ccggatctta tcgttgattt ctggcgcaat ggctgccgga
     7321 actaccactt tcgcgccatc ttcgtcaatg tacttggtgt cgtaggcata gaagtcgctg
     7381 gtgagtacga tctcgccaca ggtgctggct tgcgggttgt cgttgcccag aactgcgcat
     7441 tcgatctcac gacctttgat cccttgctca acgatcactt tatgatcgaa ttcgaacgcc
     7501 agatcgacgg caattgcgta ctgttcttca ctggttactt tgctgacacc aacagaagag
     7561 ccctgattag ccggttttac aaacagtggt aacctcagtt tagactccac ttcggcaaaa
     7621 ctaatgttgt gacgattggc gcgcgtcagg gtaataaatg gcgcaatgtt cagcccggca
     7681 tcgcgcagca gacgtttggt aacatcttta tccatacagg ctgctgaagc cagaacatca
     7741 gaacctacaa acggtaaatt ggcgacccgc agcattccct gcaaggaacc atcttcgccc
     7801 agcgtaccgt ggacaatcgg gaaaatgaca tccaccgtcg gtaacggctg accgttttgc
     7861 gcgtcgataa gctgatgctc atgtttactt ggcacctgcg caaggctagt cgccgaaggg
     7921 cgcaacgcaa tatgggcagg atcgtctgca tttagcagat aattgctggc atcgctgacg
     7981 tgccattgcc cttgtttatc aatgcccagc agcacaacgt cgaagcgact tttatcaatg
     8041 gcatcgacaa tgttttttgc cgattgcaga gacacttcat gttccgctga tttaccacca
     8101 aaaacgattc ctacccgcag tttttccatc ttaaaaacct atcccgtcta acacaaagtg
     8161 catacattac cacgacaaaa cgggggattc gcggccttct gacagaatgt tgcaatcttc
     8221 tgctgacaaa gcgtgcaacg taatggtgaa gaaagtgcgt tattacaaag atgtgcgcga
     8281 gatcacaaaa atgatgaacg ggaagctaat ttattcctgg cttaaatggc catgcggtga
     8341 gttttttctc ttagttgtaa gtaaacgcag agaatatatt tcataacttt tgtttataat
     8401 aaaggttgat aattaaaggc ctgttctttg tgtggttaat tatttaaata tgagaaacgt
     8461 ttcgctggta atcaaacaaa gaacatttgc gcaaagtatt tcctttgtca taaaaataat
     8521 acttccagac actatgaagt tgtgaaacat aatgttaact tctccatacg ttggataagg
     8581 aaatacagac atgaaaaatc tcattgctga gttgttatta aagcttgccc aaaaagaaga
     8641 agagtcgaaa gaactgtgcg cgcaggtaga agctttggag attatcgtca ctgcaatgct
     8701 tcgcaatatg gcacaaaatg accaacagcg cttgattgaa caggtggagg gggctcttta
     8761 cgaggtaaag cctgatgcca gcattcctga cgacgatacg gagctgctgc gcaattatgt
     8821 aaagaagtta ttgaagcatc ctcgtcagta aaaagttaat cttttcaaca gctgtcataa
     8881 agttgtcacg gccgagactt atagtcgctt tgtttttatt ttttaatgta tttgtacatg
     8941 gagaaaataa agtgaaacaa agcactattg cactggcact cttaccgtta ctgtttaccc
     9001 ctgtgacaaa agcccggaca ccagaaatgc ctgttctgga aaaccgggct gctcagggcg
     9061 atattactgc acccggcggt gctcgccgtt taacgggtga tcagactgcc gctctgcgtg
     9121 attctcttag cgataaacct gcaaaaaata ttattttgct gattggcgat gggatggggg
     9181 attcggaaat tacagccgca cgtaattatg ccgaaggtgc gggcggcttt tttaaaggta
     9241 tcgatgcctt accgcttacc gggcaataca ctcactatgc gctgaataaa aaaaccggca
     9301 aaccggacta cgtcaccgat tcggctgcat cagcaaccgc ctggtcaacc ggtgtcaaaa
     9361 cctataacgg cgcgctgggc gtcgatattc acgaaaaaga tcacccaacg attctggaaa
     9421 tggcaaaagc cgcaggtctg gcgaccggta acgtttctac cgcagagttg caggatgcca
     9481 cgcccgctgc gctggtggca catgtgactt cgcgcaaatg ctacggtccg agcgcgacca
     9541 gtgaaaaatg tccgggtaac gctctggaaa aaggcggaaa aggatcgatt accgaacagc
     9601 tgcttaatgc tcgtgccgac gttacgcttg gcggtggcgc aaaaaccttt gctgaaacgg
     9661 caaccgccgg tgaatggcag ggaaaaacgc tgcgtgaaca ggcacaggcg cgtggttatc
     9721 agttggtcag cgatgccgcc tcgctaaatg cggtgacgga agcgaatcag caaaaacctc
     9781 tgcttggact gtttgctgac ggcaatatgc cagtgcgctg gctaggaccg aaagcaacgt
     9841 accacggcaa tatcgataag cccgcagtca cctgtacgcc taatccgcaa cgtaatgaca
     9901 gtgtaccaac cctggcgcag atgaccgaca aagccattga attgttgagt aaaaatgaga
     9961 aaggattttt cctgcaagtt gaaggtgcgt caatcgataa acaggatcac gctgcgaatc
    10021 cttgtgggca aattggcgag acggtcgatc tcgatgaagc cgtacaacgg gcgctggaat
    10081 tcgctaaaaa ggatggcaac acgctggtca tagtcaccgc tgatcacgcc cacgccagcc
    10141 agattgtcgc gccggatacc aaagctccgg gcctcaccca ggcgctaaat accaaagatg
    10201 gcgcagtgat ggtgatgagc tacgggaact ccgaagagga ttcacaagaa cataccggca
    10261 gtcagttgcg tattgcggcg tatggcccgc atgccgccaa tgtcgttgga ctgaccgacc
    10321 agaccgatct cttctacacc atgaaagccg ccctggggct gaaataaaac cgcgcccggc
    10381 agtgaatttt cgctgccggg tggttttttt gctgttagca accagactta atggcaaatc
    10441 tcgggcgcat acgctcatgg ttaaaacatg aagagggatg gtgctatgaa aattacatta
    10501 ctggttacct tgcttttcgg tctggttttt ttaaccacag tcggcgctgc cgagagaacg
    10561 ttaaccccac aacaacagcg tatgacctcc tgtaatcagc aggcgacggc gcaggcgttg
    10621 aaaggggatg ctcgtaagac ctacatgagt gattgcctga agaacagcaa gtctgcgcct
    10681 ggtgaaaaaa gtttgacgcc acagcagcaa aagatgcgcg aatgtaataa tcaagcaacg
    10741 caacaatctc tgaaaggtga tgatcgtaat aagtttatga gtgcctgcct caagaaagcc
    10801 gcctgatacc tgatagtgct aacgggtgag ctacgaaagt ggctcacccg aaatttcata
    10861 cttctgcctt tagccccgtc tctataattt gggaaaattg tttctgaatg ttcccaaaaa
    10921 taatgaatga tgaaaacttt ttcaaaaaag cggcggcgca tggggaggaa cctcctttaa
    10981 ctcctcaaaa cgaacatcag cggtccgggc tgcgcttcgc ccgtcgcgtc agactacccc
    11041 gtgcggttgg cctggctggc atgttcttac cgattgcttc aacgctggtt tcgcacccgc
    11101 cgccgggctg gtggtggcta gtgttggtcg gctgggcgtt cgtctggccg catttagcct
    11161 ggcagatagc gagcagggcc gtcgatccgc ttagccggga aatttacaac ttaaaaaccg
    11221 atgcagtatt agcgggaatg tgggtaggcg taatgggcgt aaacgtgctg ccttccaccg
    11281 ctatgttgat gattatgtgt ctgaatttga tgggggcagg cggcccccgt ctgtttgtcg
    11341 cgggtctggt gttgatggtg gtttcctgcc ttgtcaccct cgagcttacg ggcattaccg
    11401 tgtcgttcaa tagtgcgccg ctggaatggt ggctctccct tcccattatt gtcgtttatc
    11461 ctctgctgtt tggctgggtc agctaccaga cggcaaccaa actggcggaa cataaacgca
    11521 ggttgcaggt catgagtacc cgcgacggca tgacgggcgt gtataaccga cgtcattggg
    11581 aaactatgtt acgcaatgaa tttgataact gtcggcggca taatcgcgat gcaacgttac
    11641 tgattatcga tatcgaccat ttcaagagca tcaacgatac ctggggtcat gatgtgggcg
    11701 atgaagcgat tgtggcgctt acccgacagt tacaaattac cctgcgcggt agcgatgtga
    11761 ttggtcggtt tggcggcgat gagtttgcag taatcatgtc cggtacgcca gctgagagcg
    11821 ccattaccgc catgttacgg gtgcgtgaag ggctaaacac gttacgcttg ccgaatacgc
    11881 cacaggtaac tttacggatt agtgtggggg ttgcgccgct gaacccacaa atgagtcact
    11941 atcgtgagtg gttgaaatcg gcagatttgg cgctttacaa agcaaagaaa gccggacgta
    12001 accgcaccga agtggcggcc tgacgtcagg cgaaagtcat caggatttgc tgagtttttc
    12061 tgatttttcc atacacttcg tcatcgcttc gatcactgcg gcacggaagc ctttctcttc
    12121 cagtacgcgt accgcttcaa tggtggtgcc tcccggtgag cagaccatat ctttcagtgc
    12181 ccccggatgt tctcccgttt ccagcaccat ttttgcggaa cccattaccg cctgagcggc
    12241 aaatttatac gcctgggcgc gtggcatccc gcccagcacg gcggcgtcgg ccatcgcttc
    12301 gataaacata aatacgtagg ctggcgaaga accgctcaca ccgactaccg ggtggatcat
    12361 cggctcagca attacttccg cttcgccaaa gcagcggaaa atattcagca catcagcggt
    12421 atcttctggg gttaccagcg cgtttggcgt tacggaggtc atcccggcat taaccagcgc
    12481 gggagtgttc ggcatggcgc ggataatttt ccggtcatgg cccagcgcgc gggcaagctg
    12541 gtcgagcgtg acacctgcag caatagaaac gaccagagag tctttattca ggctggaggt
    12601 gatttcgcta agcactttaa tcatgatgcc aggtttaacg gcagcaaaaa tgatgtcggc
    12661 gatttgcgcc acttcttgcg ccgattctgc ggcgttgatg ccgaactggt catgcagggc
    12721 ggcgacttta ttcggggagg gggtgtatac ccagatttgc cctggaagca cctgaccgct
    12781 ggcaatcaga ccgccgagaa tggcttttcc catattgccg cagccaataa aaccgatttt
    12841 cttttccatt gcctcactcc tgccgtgaaa ttcattgttt tgataatcgc tggcagaagc
    12901 ataaacagaa ctatgccgga aggcaaaagc gcgacacaat agaggattac ccaacaaagg
    12961 atgactttat gacaatttgg gtggatgctg acgcgtgtcc caatgtaatt aaagagattt
    13021 tgtatcgcgc ggcggaacgt atgcagatgc cgctggtact ggtagcaaac cagagtttac
    13081 gcgtgccgcc atcgcgattt attcgtacgc tgcgcgtcgc ggcaggtttc gacgttgccg
    13141 ataacgaaat tgtccggcag tgtgaagcgg gcgatttggt gatcaccgca gatatacctt
    13201 tggctgctga agccatcgag aaaggcgcag cggcgcttaa tccgcgcggc gaacgttaca
    13261 cgccagcgac cattcgtgag cgcctgacga tgcgcgattt tatggatacc ttacgtgcca
    13321 gtgggatcca gaccggcgga ccagatagcc tttcacaacg tgaccgccag gcctttgccg
    13381 cggagctgga gaagtggtgg ctggaagtgc aacgtagtcg tggctaaatg taatttatta
    13441 tttacacttc attcttgaat atttattggt atagtaaggg atgtattgag attttcactt
    13501 taagtggaat tttttcttta caatcgaaat tgtactagtt tgatggaatg atcgctattc
    13561 tcatgacacc ggctttcgcc gcattgcgac ctattgggga aaacccacga tgacacaacc
    13621 tctttttctg atcgggcctc ggggctgtgg taaaacaacg gtcggaatgg cccttgccga
    13681 ttcgcttaac cgtcggtttg tcgataccga tcagtggttg caatcacagc tcaatatgac
    13741 ggtcgcggag atcgtcgaaa gggaagagtg ggcgggattt cgcgccagag aaacggcggc
    13801 gctggaagcg gtaactgcgc catccacagt tatcgctaca ggcggcggca ttattctgac
    13861 ggaatttaat cgtcacttca tgcaaaataa cgggatcgtg gtttatttgt gtgcgccagt
    13921 atcagtcctg gttaaccgac tgcaagctgc accggaagaa gatttacggc caaccttaac
    13981 gggaaaaccg ctgagcgaag aagttcagga agtgctggaa gaacgcgatg cgctatatcg
    14041 cgaagttgcg catattatca tcgacgcaac aaacgaaccc agccaggtga tttctgaaat
    14101 tcgtagcgcc ctggcacaga cgatcaattg ttgattttag agcgcctata cttaacgttc
    14161 attccgtgaa ataaggaaga acgatgccaa cgaaaccgcc ttatcctcgt gaagcatata
    14221 tagtgacgat tgaaaaagga aagccaggac agacggtaac ctggtaccaa ctcagagccg
    14281 atcatcctaa accagactcg ttgatcagtg aacatccgac cgctcaggaa gcgatggatg
    14341 cgaaaaaacg ctatgaggac cctgacaaag agtgaccgca tcagactgct cggaagggat
    14401 tctgagtgcc actacaaggg atctgcgtca catttttcat aattcatgtt tttctaataa
    14461 ttagaatatt aaacaataac aatccattac tggaatcatt tggaatcttt acattatgcc
    14521 gtgcacgtct gctgctacgc tttttgtcat ttgtagcaca agtaagtgtc agcagtggtg
    14581 cttcacactt gcccggtaat taacgacgaa agaaaagtaa ggtggatgaa caatgagtgc
    14641 gtcgttggcg atcctgacca tcggcattgt acctatgcag gaggttttgc cgctcctgac
    14701 ggaatacatt gacgaagata atatttccca tcatagcctg ctggggaagt taagtcgtga
    14761 agaagtgatg gcggagtacg cgccagaagc aggcgaagac accattctca cattattaaa
    14821 tgacaaccag ctggcccatg tttcgcgtcg taaagtggag cgagacctgc aaggtgtggt
    14881 tgaagtgctc gataatcagg gttatgacgt cattttatta atgagtacag caaacattag
    14941 tagtatgact gcgcgtaata cgatctttct tgagccgtcg cgaatattgc ctccactggt
    15001 ttcctctatt gttgaagatc atcaggtggg ggttatcgtt ccggttgagg agatgctgcc
    15061 cgttcaggcg caaaaatggc aaattttgca gaaatcgccg gtattttcat tgggtaaccc
    15121 cattcatgat tcagaacaaa aaatcattga tgcagggaaa gaattactgg caaaaggggc
    15181 tgatgtcatc atgctggatt gtttaggatt tcaccaacgt catcgcgatt tactgcaaaa
    15241 acagctcgat gttcctgtct tgctgtctaa cgtattgatt gcacggctgg ctgcggaatt
    15301 actggtgtaa ttttgcgtga cagccagcgc ctctggcccc tatagtgaag tagatgttca
    15361 actaccagac agggccagtt tatgcttcaa agtaatgagt acttttccgg caaagtgaaa
    15421 tcaatcggct tttccagcag cagcactggt cgcgccagcg tgggtgttat ggttgaaggc
    15481 gaatacacct tcagcaccgc tgagccggaa gagatgacgg taatcagtgg cgcgctgaat
    15541 gtgttactgc ctgatgcgac cgactggcag gtgtatgaag ccggttcggt gtttaatgtt
    15601 cccggtcaca gtgagtttca tctgcaagtt gccgaaccca cctcttatct gtgccgctat
    15661 ctgtaattcc tcgccttccc cttgaacggg agggcatttt tctgaaatat cctctcttta
    15721 acccataata atatttcctt tgctgcgatt ttttcaattt ccgatatatt cataattcat
    15781 caaggttgat ataaatatca gtgaagatct ccagatattg ttgcggaact ggctacgata
    15841 aaagataaat cagatgatga atggtggcgt gcattgctgc aaagtcgcaa tgatcatttg
    15901 cgccaaacgg cattacgcaa cgcacatacc ccggcgtcgt tgttaacgac attgaccgag
    15961 tctcggcatc ggtcgctagc tatgaataat ccacagctcg ctgccgatgt gaaaacgacg
    16021 tggttaaaag aggatccatc attactctta tttgtcgaac aacccgatct ttcgctgtta
    16081 cgggatttag tgaaaaccgg ggcaatgcgg aaaattcgca gtgaagcgcg tcactggctt
    16141 gaggaaaaac aatgacacaa cgtccatgga gcaagttaca gcataaaacg cataatattg
    16201 ctgcgctaaa aattattgtc cgccgtagcg aataattata tccctggtgt ggcttcgtac
    16261 gccggataag acgcggcagg cgtcgcatcc ggcattaaag gaaaatcagc aattaacgtt
    16321 gtgcttcgcc acctaatcct tcaatcaggt tttgaattaa cgctgccagt tcaccagtca
    16381 tcaggatgaa atcggcatca aaacgctggg cgaaatcttc acggtcgata tcttcgtttt
    16441 gatcgcgcag ctcgtcgcaa aacttcagac gcttgagcga accatcgtcg cacatcacaa
    16501 actgaatgcg ctgctgccag tcgagcgcca gtttagtcac cacttttccg gcttcgatgt
    16561 gattggtgat ctcttcgctg gtcagatctt gtttcttcgc gcggatcacg ccgccatctt
    16621 ccagcaacga tttcagctcg gcttcatcaa gcagctggaa gccctgtgcc gcgctaccgg
    16681 agcgaaccca ttcggtcagc gtcagttcaa tcgggttttc catgctcaat ggtaccacag
    16741 gtaacgaccc caggctttta cgcagcagtg ccagcgtatc ttccgctttt ttggcactgg
    16801 cgcagtccac cataatcaaa ccgttaaccg tgtcgatcca catcattgtc tggctaaaac
    16861 ggctgaaagc acgcggcaac agagagtgca gcacttcgtc tttcagcgaa tctttttcgg
    16921 ttttcttcag cttacgcgcc tgttccgctt ccagtttggc gattttcgct tccagcgcct
    16981 gcttaatcac cggagacggg aggatttttt cttctttgcg cgcgcagata acaatttgac
    17041 cattggcaac gtgcgttaac gcatcgctgt gcgatcccat cggaggaacc cagcccatct
    17101 tcgccatgtc ctggctgccg catggggtaa atgccatcga ggctagctgt ttttccatct
    17161 cttctgcacg cagcgaaatc tcgcggctaa gacggtaaac cattaaattt ttgaaccaca
    17221 gcatgataat ttccacggcc ttgttgttaa atttagcggg catgataacg aattgtcggc
    17281 ggccttgcat tgccaatccg gttgtccgtc tctacgctat tgatattgaa aaaaataagg
    17341 agagtaccgt gcgtataggt atcgatttag gcggcaccaa aactgaagtg attgcactgg
    17401 gcgatgcagg ggagcagttg taccgccatc gtctgcccac gccgcgtgat gattaccggc
    17461 agactattga aacgatcgcc acgttggttg atatggcgga gcaggcgacg gggcagcgcg
    17521 gaacggtagg tatgggcatt cctggctcaa tttcgcctta caccggtgtg gtgaagaatg
    17581 ccaattcaac ctggctcaac ggtcagccat tcgataaaga cttaagcgcg aggttgcagc
    17641 gggaagtgcg gctggcaaat gacgctaact gtctggcggt ttcagaagca gtagatggcg
    17701 cggcagcggg agcgcagacg gtatttgccg tgattatcgg cacgggatgc ggcgcgggcg
    17761 tggcattcaa tgggcgggcg catatcagcg gcaatggcac ggcaggtgag tggggacaca
    17821 atccgctacc gtggatggac gaagacgaac tgcgttatcg cgaggaagtc ccttgttatt
    17881 gcggtaaaca aggttgtatt gaaaccttta tttcgggcac gggattcgcg atggattatc
    17941 gtcgtttgag cggacatgcg ctgaaaggca gtgaaattat ccgcctggtt gaagaaagcg
    18001 atccggtagc ggaactggca ttgcgtcgct acgagctgcg gctggcaaaa tcgctggcac
    18061 atgtcgtgaa tattctcgat ccggatgtga ttgtcctggg gggggggatg agcaatgtag
    18121 accgtttata tcaaacggtt ccgcagttga ttaaacaatt tgtcttcggc ggcgaatgtg
    18181 aaacgccggt gcgtaaggcg aagcacggtg attccagcgg cgtacgcggc gctgcgtggt
    18241 tatggccaca agagtaaaaa acgtaggcaa ttggcgcatc atgcctgatg cgacgcttgc
    18301 cgcgtcttat caggcctgca aaatgtgcca gaaccgcgta gggcggataa ggcgttcacg
    18361 ccgcatccgg caataagtaa tgaacaccga gactataacc taccccagtg gtttcgccag
    18421 caccggagta tccgccgctt gctggcgctt atagcgacca tacagcagca acgacgacat
    18481 cgcagcaaac gaaagcaggg cggcaggcag cgccacgtaa ttatatgcca gccccagcgt
    18541 cagcatcata ccaccgcaat atgcgccgac ggcgctaccg aggttaaacg ctatttgccc
    18601 acctgcggca cctaataact ctccgccttt ggcgttttgt agtaacaata tttgtagcgg
    18661 tgctgaaagg gcaaataatc ccgcgcaaca aataaaagca aaaataagcg acgttgtttt
    18721 catgccgccg cagaaaaaga acatcagcag tgccagtaca attataaagt cagtcactgc
    18781 tgcaatgcgc agtggtgaat aacgtcctga aatcctgcca cttagcatat ttcccagcac
    18841 catccctagc ccaactaaca tcataataaa ggtcatcgcc gtttccgaaa aaccggaaat
    18901 aaacatcatg tatggcttta cgtagctgaa ccaggcaaac acacctgcgt tgccaaacat
    18961 ggtggcggcg aaaattaacc acggggccgg gctgcgcaaa aagtgaaatt gttcgcgcag
    19021 ctttcctttc gcctcgtcgc gaatatccgg cacccaaaaa tagaccgatg ccatcaccgc
    19081 aatattaaaa acagcgatca ataaaaaggt gtaacgccag ctaaattcct gacttaaata
    19141 cgttcccagc ggaatgccca gcaaattggc gactgtcatc ccggaaacca tccccgccac
    19201 ggcggcggtg acttttccgg gtttgataat ttttgataac acgatcgcgc cgacgccaaa
    19261 aaatgcgcca tgcggaaagc cggataccag ccgaccaatg gcgagcatca ggtaagacga
    19321 agagagcgtg aacatggcgt tgccaatgac gcacaacgcc accagaaaca acaagatatg
    19381 tttgagtgag tagcggctgg aaaagagtgc gatgattggc gcaccgacca ccactcccag
    19441 tgcataatac gagatcatat gcccggcagc aggaatcgaa attcctacgt tatgcgccag
    19501 ctccgtgagc acgcccataa tgccaaattc ggccatcccc aaaccaaacg tgcccagagc
    19561 caaagataaa atgacttttt tcataccacc atcacaacca ccagaacggc taccaatcac
    19621 cgtagccaat aaagtgatag cttgcaggat aacgaccagc aaggccaaga gataattcgc
    19681 ccctctgtat tcattatcct gctgaatagt tatttcactg caaacgtact ttccagtttg
    19741 ctgtagccca ggccgttgat ctttttcact ttgatctgca ccggaatacg ctctttcatc
    19801 gcttctacgt ggctaatcac accgatggtt ttgccactgg cgttcagggc atccagcgca
    19861 tcaagggcgg tatccagcgt ttcgctatcc agcgtgccaa aaccttcatc aaggaacagc
    19921 gagtcaatac gtgttttatg gctgaccaga tccgaaagcg ccagcgccag actaacgagg
    19981 aaactttcgc cgccggaaag ggtacgggta tcgcgtaccg catctgcctg ccaggtatca
    20041 acaacctcga cttccagcgc ctcgctggct ttgcgctgta acagatagcg cccgtgcagc
    20101 cgggtaagtt gctgattagc gagatggact aaattatcca gcgtcagccc ctgggcaaac
    20161 ttgcggaatt tatcgccctc tttggaacct attagcgaat tcagatatcc ccagtcctca
    20221 acctgctgcg tcatttgagc aatttgctgc attaaggttt gttgttgctg acggttatct
    20281 gcatcctgct tcagctgctg gcgaatctcg ccttgactcg tggtgttttc acgcaacttt
    20341 tggtgagttt gcgctaactc ttgctgaatc tgctccaccg tcacagtgag agccaacccg
    20401 tcaggtcggt gttgttgatg ctgtgccagc gtttctgctg tctgagtgac cagcgtttgc
    20461 gcctgacggc gctggttttc cagattctgc ttgagctgtt ccagctgcgt tagtgtttgt
    20521 tcatccatta gcgccgcaag gaacgcctgc tgatcgtcaa agacgctggc ctgtagcgcg
    20581 gtgtcaaact gcgcctgggc tttttgcaga ctttgcgccg ccagaacatc ctgttgctgt
    20641 aacgtctgct gctggctgtg taatgcgaga cattgttcat gaacctgccg ccagttatcc
    20701 agcgccacag tttcttcgct gtgcgggaga tcatcacttt gcggcaacgt ttccagaatc
    20761 ggcgtcagct gctgaatacg gttttgcagc gtggttaatt cgttctggcg ttgctgccag
    20821 ctctgcgctt cttgctgacg tgtcgccaac cagctctctt cttcatcttc ctgtggcaat
    20881 gtcagtgcat aacccgccaa tgccgttaag agctgctgct ggcgttgttc aatttgctgt
    20941 tgatactgga taatttgctg attatgcgcg gcaatctgcc cttgtaattc atgtcgttgg
    21001 ctgagtaacc gcagctggcg tttgtgctca tcttgtgcat ccagccacgg ttgaatatcg
    21061 tcctgtggct gcaaggtgat attgaggctg gccgtgacgg cttgccattg ttgagtaagt
    21121 gcttgctcat cttgtcggag gctttgcgct tcgttttcat cgcgctgaag ctgcttcgtt
    21181 aatgcatcca gttgcccgcg cagcgccgca ccttcttcgc cgagcttttt aacttcgttt
    21241 tccagcgcca gtaatcgaga ctgattaaca ccaggctcca gcgcctgata cgccttgacc
    21301 gccgggtggc tggtggaacc acaaagtgga caaggctgac ccgcctgtaa ctgtgcacgc
    21361 tgggcttcca gcgttttgat gcgcgcttcc tgctcgcaaa tggttttcac atcggcaagt
    21421 tgctgcgtct tttctttata acgctggcgc atttcgttaa gtgcgacatt acgttgcgtc
    21481 tgttccagag atccattctg gatagtgacc attaactgcg ccagacgttt ttgttgggga
    21541 acaatctgcc catgcagcgc gaccaggcgc tgacgcagtg ggcgttgctc agcatgttgc
    21601 gccagggcgg tagcaacttc atcggcggtt aacgtcaacg tgatcgccgc aagcgcatta
    21661 agtttttgct cagcatgggt taactgttgc tgccattgcc gcagatgctc gcgatcgctg
    21721 gtttgttggg agaactgcgc acgccaaccc gccagttcgt tgttccactg acggaagcgg
    21781 tcgtgttcct gtaaccaggt attcaggctt tgttgctgct gctgtaattc tgctgactgc
    21841 ttcgccgcgt ggtggcgaat gctcgcgcga agcgccattg tgctctgtaa gcgagtattt
    21901 acttcttcaa tctgctggcg agtatgcgcc agcgccgcgc tgtgttctgc gatgcgttcc
    21961 cagtgcggac gaagatttcg tgccggttgt gccagactga gcgccgccag ttgaggttgc
    22021 gctttttctt cttcggctaa cgcctgttgc aaggcctgct gacggcggct ggcttcttgc
    22081 tgcaattcgt ccagacgcgt taaccagttt agtgattgtt gttcttgctg ctgcgcggta
    22141 agtaactgtt tttcttcgtc agtaagtacc tgcaaactcg ctgtcagcga ttgcacttgt
    22201 tccggcgtga gcaacgcgac gccgctggcc tgcgcttgca gcttctccag ctctgtgcgg
    22261 gccgatttgt gctgctcaaa aaccatcgcc gagatttgcc cgtagatttc agtgccggtt
    22321 aactcctcga gcaattccgc gcgttctttg ggtttggcat tcaggaaggc agcaaattgc
    22381 ccctgcgaaa gcagcatcga acgggtgaag cgcccgtaat ccagcccggt taacgtcgct
    22441 gtcagttcca gcttatcttt cactttgtcg gcgagaattt tgccgtcggc gcagcgcgcc
    22501 agctctacgc gtggcacctg caaattaccg tcgggttggt tacgcgcccg attctggctc
    22561 cagaatgcac ggtacgcttc acctttcact tcaaactcca cctccgccag acattcggcg
    22621 gtatcgcggg tcatgagatc attttgcgat tgtgaaacgt tagagagacg cggagtttcg
    22681 tgatacagcg ccagacaaat ggcgtccagc agggtggttt tccccgcacc ggttgggccg
    22741 gtaatggcaa acagcccgtt gctggcgaac ggctcgcggg tgaaatcaat cttccattcg
    22801 ccttttaatg agttcaggtt tttcaggcgc aggctgagaa ttttcatgct tcgtgttctc
    22861 cggcgagggt atgcaacgtc gtggtgaaaa gatgctgcag acgttgctgc tgcgattcat
    22921 ccagttcttc cagtgccaga cggcgattga acacctcttc gacgctgagt tcgctgaggg
    22981 tttcacgctg ttggctggct aacacacgct cgcgctgttc acgactccga cgtaccagca
    23041 atacttcgac aggcaatgat tcggctaatg cctggatttt gcgctgaata tcatgcagat
    23101 actcatcagt agtgatttcg atatccagcc agacaggtgg ctcctgcgat acatcgcgcc
    23161 actgttccag ctgtgcggta atcgacgcca gatcgccttt cagcaccgcc atgggttgag
    23221 ttaccggtac gttcaggttt tccacgctct ctaatttgcc gtttgaaaat gtcaccagat
    23281 ggacatattt actcttaccg cattcatcaa aactcagtgg aatgggggaa ccgcaatagc
    23341 gaacatgttc catgccgcca ataatctgtg cgcggtgaat atgcccgagc gcgatgtagt
    23401 cggctggtgg aaagttttgt gccggaaacg cgtccagcgt gccaatataa atgtcacgca
    23461 cggcgtcact tttactggca cccacggtcg ttaaatgtcc cgtggcgatg atgggcagag
    23521 gctgatcgcc gcgcagtttg caggcatcgg catagtgttg ttggtaataa tcggtaatcg
    23581 ctgccagtaa atgctgctgt ttttcaatac cgttaagccc cgcctggctg gaaataatgt
    23641 cacgcgggcg taaaaacgga atggggcaca gcactgcgcc tggcgtcccg tcgcgacgag
    23701 gcaagatttg cggcgcatgt ccggcgctgg cgaccacggt tgtattgagg aacgccatga
    23761 tatcgcgcga ttcattcagc gtggcgaccg agtcatggtt tcctgccagt accaccagat
    23821 gacagccagt ttgctgtaaa ttgacaacaa aacggttgta taacgtgcgg gcgtaactgg
    23881 gcggcgagcc ggtatcgaaa acatcaccag caacaataat cgcatccacc tgatgggttt
    23941 gtgctgtctc cagcagccag tcaagaaaag cctgatgttc agcttcgcgg cttttactgt
    24001 agaagttctg gccgagatgc cagtctgagg tgtgaaggat gcgcataacg gttccctggc
    24061 gaaaaagcat ggtcgcgatt atacccaaac agatgtgcca tttgcttttt tctgcgccac
    24121 ggaaatcaat aacctgaaga tatgtgcgac gagcttttca taaatctgtc ataaatctga
    24181 cgcataatga cgtcgcatta atgatcgcaa cctatttatt acaacagggc aaatcatggc
    24241 gagacgtatt ctggtcgtag aagatgaagc tccaattcgc gaaatggtct gcttcgtgct
    24301 cgaacaaaat ggctttcagc cggtcgaagc ggaagattat gacagtgctg tgaatcaact
    24361 gaatgaaccc tggccggatt taattctcct tgactggatg ttacctggcg gctccggtat
    24421 ccagttcatc aaacacctca agcgcgagtc gatgacccgg gatattccag tggtgatgtt
    24481 gaccgccaga ggggaagaag aagatcgcgt gcgcggcctt gaaaccggcg cggatgacta
    24541 tatcaccaag ccgttttcgc cgaaggagct ggtggcgcga atcaaagcgg taatgcgccg
    24601 tatttcgcca atggcggtgg aagaggtgat tgagatgcag ggattaagtc tcgacccgac
    24661 atctcaccga gtgatggcgg gcgaagagcc gctggagatg gggccgacag aatttaaact
    24721 gctgcacttt tttatgacgc atcctgagcg cgtgtacagc cgcgagcagc tgttaaacca
    24781 cgtctgggga actaacgtgt atgtggaaga ccgcacggtc gatgtccaca ttcgtcgcct
    24841 gcgtaaagca ctggagcccg gcgggcatga ccgcatggtg cagaccgtgc gcggtacagg
    24901 atatcgtttt tcaacccgct tttaacgcct tgctcatcgg acgcagagca gggcttatga
    24961 tttcttaact ggagtatctt acgtgctgga acggctgtcg tggaaaaggc tggtgctgga
    25021 gctgctactt tgttgcctcc cggctttcat cctgggtgca ttttttggtt acctgccctg
    25081 gtttttgctg gcatcggtaa caggactgct tatctggcat ttctggaatt tattgcgcct
    25141 ttcatggtgg ttgtgggtgg atcgcagtat gaccccgcca ccggggcgtg gtagctggga
    25201 accgctacta tacggcttac accagatgca gctgcgaaat aaaaaacgcc gccgtgaact
    25261 gggcaatctg attaaacgct ttcgtagcgg cgcggagtcg ctgcctgacg cggtggtgct
    25321 gaccacggaa gagggcggta ttttctggtg taacggtctg gcgcaacaaa ttcttggttt
    25381 gcgctggccg gaagataacg ggcagaacat ccttaaccta ctgcgttacc cggagtttac
    25441 gcaatatctg aaaacgcgtg atttttctcg cccgctcaat ctggtgctca acaccgggcg
    25501 gcatctggaa attcgcgtca tgccttatac ccacaaacag ttgctgatgg tggcgcgtga
    25561 tgtcacgcaa atgcatcaac tggaaggggc gcggcgtaac ttttttgcca acgtgagcca
    25621 tgagttacgt acgccattga ccgtgttaca gggttacctg gagatgatgg atgagcagcc
    25681 gctggaaggc gcggtacgcg aaaaagcgtt gcacaccatg cgcgagcaga cccagcggat
    25741 ggaagggctg gtgaagcagt tgctaacgct gtcgaaaatt gaagccgcgc cgacgcaatt
    25801 gctcaatgaa aaggttgatg tgccgatgat gctgcgcgtt gttgagcgcg aggctcagac
    25861 tctgagtcag aaaaaacaga catttacctt tgagatagat aacggcctca aagtgtctgg
    25921 caacgaagat cagctacgca gtgcgatttc gaacctggtc tataacgccg tgaatcatac
    25981 tccagaaggc acgcatatca ccgtacgctg gcagcgagtg ccgcacggtg ccgaatttag
    26041 cgttgaagat aacggaccgg gcattgcacc ggagcatatt ccgcgcctga ccgagcgttt
    26101 ttatcgcgtt gataaagcgc gttcccggca aaccggcggt agcggattag ggttagcgat
    26161 cgtgaaacat gctgtgaatc atcacgaaag tcgcctgaat attgagagta cagtaggaaa
    26221 aggaacacgt ttcagttttg ttatcccgga acgtttaatt gccaaaaaca gcgattaatc
    26281 cgcctttgtc atcttttatt gccataagcc agtcgatgct ggcttatttt ctttgcagtc
    26341 aaaatacggg cgttagattt tacaacggtt ggtgattttt tgttcgcatg attagccatg
    26401 tctttttcac ggaaatagtg ttttatactg gttggtgatt tcttatcgct atatacctct
    26461 ggtttttaga tccctccttg ctttaaaacg ttataagcgt ttaaattgcg cttcaggtgc
    26521 tgtcatactg actgcattaa cgcggtaaat cgaaaaacta ttcttcgccg cgccaggttg
    26581 ggagtatttc ccgctaaaat tgtttaaata taccgctgta tcatccccag ggattggcac
    26641 aaaaatttaa cgttacaaca ccacatccac aggcagtatg atttatgacc catcaattaa
    26701 gatcgcgcga tatcatcgct ctgggcttta tgacatttgc gttgttcgtc ggcgcaggta
    26761 acattatttt ccctccaatg gtcggcttgc aggcaggcga acacgtctgg actgcggcat
    26821 tcggcttcct cattactgcc gttggcctgc cggtattaac ggtagtggcg ctggcaaaag
    26881 ttggcggcgg tgttgacagc ctcagcacgc caatcggtaa agtcgctggc gtgctgctgg
    26941 caacggtttg ttacttggcg gtggggccgc ttttcgctac gccgcgtaca gctaccgttt
    27001 cctttgaagt ggggattgcg ccgctgacgg gtgattccgc gctgccgctg tttatctaca
    27061 gcctggtcta tttcgctatc gttattctgg tttcgctcta tccgggcaag ctgctggata
    27121 ccgtgggcaa cttccttgcg ccgctgaaaa ttatcgcgct ggtcatcctg tctgttgccg
    27181 ctattgtctg gccggcgggt tctatcagca cggcgactga ggcttatcaa aacgctgcgt
    27241 tttctaacgg cttcgttaac ggctatctga ccatggatac gctgggcgca atggtgtttg
    27301 gtatcgttat tgttaacgcg gcgcgttctc gtggcgttac cgaagcgcgt ctgctgaccc
    27361 gttataccgt ctgggctggc ctgatggcgg gtgttggtct gactctgctg tacctggcgc
    27421 tgttccgtct gggttcagac agcgcgtcgc tggtcgatca gtctgcaaac ggcgctgcta
    27481 ttctgcatgc ttacgttcag cacacatttg gcggcggcgg tagcttcctg ctggcggcgt
    27541 taatcttcat cgcctgcctg gtaacggcag ttggcctgac ctgtgcttgt gcagaattct
    27601 ttgcccagta cgtaccgctc tcttatcgta cgctggtgtt tatcctcggc ggcttctcga
    27661 tggtggtgtc taacctcggc ttaagccagc tgatccagat ctccgtaccg gtgctgaccg
    27721 ctatttatcc gccgtgtatc gcactggttg tattaagttt tacacgctca tggtggcata
    27781 attcgtcccg cgtgattgcc ccgccgatgt ttatcagcct gctttttggt attctcgacg
    27841 ggatcaaagc atctgcattc agcgatatct taccgtcctg ggcgcagcgt ttaccgctgg
    27901 ccgaacaagg cctggcgtgg ttaatgccaa cagtggtgat ggtggttctg gccattatct
    27961 gggatcgcgc ggcaggtcgt caggtgacct ccagcgctca ctaaatcact gaacatttgt
    28021 tttaaccacg gggctgcgat gccccgtggt tttttattgt gttgatgggt taggaattga
    28081 tggaaagtaa gaacaagcta aagcgtgggc taagtacccg ccacatacgc tttatggcac
    28141 tgggttcagc aattggcacc gggctgtttt acggttcggc agacgccatc aaaatggccg
    28201 gtccgagcgt gttgttggcc tatattatcg gtggtatcgc ggcgtatatc attatgcgtg
    28261 cgctggggga aatgtcggta cataacccgg ccgccagctc tttctcgcgt tatgcgcagg
    28321 aaaacctcgg cccgctggca ggttacatta ccggctggac ctactgcttt gaaatcctaa
    28381 ttgtcgccat cgccgatgtg accgcttttg gtatctatat gggtgtctgg ttcccgacgg
    28441 tgccgcactg gatttgggta ctgagcgtgg tgctgatcat ttgcgccgta aacctgatga
    28501 gcgtgaaggt attcggtgag ctggaattct ggttctcgtt ctttaaagtc gccaccatca
    28561 tcatcatgat tgtcgccggt ttcggcatca tcatctgggg gattggcaac ggcgggcaac
    28621 cgaccggtat tcataacctg tggagcaacg gcggcttctt cagtaacggc tggcttggca
    28681 tggtaatgtc gttgcaaatg gtgatgtttg cttacggtgg gatcgaaatt atcgggatta
    28741 ccgccggtga agcgaaagat cctgagaaat cgattccgcg tgcgattaac tccgtgccga
    28801 tgcgtattct ggtgttctac gtcggtacgc tgttcgtcat tatgtctatc tacccgtgga
    28861 atcaggttgg cactgccggt agcccgttcg tgctgacgtt ccagcatatg ggcattacct
    28921 ttgccgccag cattcttaac tttgttgtgc tgaccgcttc gctgtcggca attaacagtg
    28981 acgtatttgg cgtaggccgt atgctccacg gtatggcaga gcagggcagc gcaccgaaaa
    29041 ttttcagcaa aacgtcgcgt cgcggtattc cgtgggttac ggtgctggtg atgactaccg
    29101 cgctgctgtt tgcggtgtat ctgaactaca tcatgccgga aaacgtcttc ctggtgatcg
    29161 cttcgctggc aaccttcgcc acggtatggg tgtggattat gatcctgctg tcgcaaatcg
    29221 ccttccgtcg ccgtttgccg ccagaagaag ttaaggcgct gaaatttaaa gtgccgggtg
    29281 gggtagcaac gaccatcggc gggctgattt tcctgctctt tattatcggg ttgattggtt
    29341 atcacccgga tacgcgtatc tcgctgtatg tcggtttcgc gtggattgtt gtgctgttga
    29401 ttggctggat gtttaagcgt cgccacgatc gtcagctggc tgaaaaccag taatccttgc
    29461 gttctgtaag ccggttcagg aggaaatcct gatccggcta ttccgaaagt tatccgcccc
    29521 cgtcctcctc ccccaaatat ccttcagatg atgagtgatc ctgcattagg ctatggcaag
    29581 gtgatcagat tttcatcaca ggggaattat gatgttaaat gcatggcacc tgccggtgcc
    29641 cccatttgtt aaacaaagca aagatcaact gctcattaca ctgtggctga cgggcgaaga
    29701 cccaccgcag cgcattatgc tgcgtacaga acacgataac gaagaaatgt cagtaccaat
    29761 gcataagcag cgcagtcagc cgcagccagg cgtcaccgca gggcttcagc cgaatgccgc
    29821 cggcacgact ggagcagttt gccgtcgatg taccggatat cggcccacaa tgggctgcgg
    29881 atcagatttt ttatcagatc ttccctgatc gttttgcgcg tagtcttcct cgtgaagctg
    29941 aacaggatca tgtctattac catcatgcag ccggacaaga gatcatcttg cgtgactggg
    30001 atgaaccggt cacggcgcag gcgggcggat caacgttcta tggcggcgat ctggacggga
    30061 taagcgaaaa actgccgtat ctgaaaaagc ttggcgtgac agcgctgtat ctcaatccgg
    30121 tgtttaaagc tcccagcgta catcaatacg ataccgagga ttatcgccat gtcgatccgc
    30181 agtttggcgg tgatggggcg ttgctgcgtt tgcgacacaa tacgcagcag ctgggaatgc
    30241 ggctggtgct ggacggcgtg tttaaccaca gtggcgattc ccatgcctgg tttgacaggc
    30301 acaatcgtgg cacgggggga gcttgttaca accctgaatc gccctggcgc gactggtact
    30361 cgtttagtga tgatggcacg gcgctcgact ggcttggcta tgccagcttg ccgaagctgg
    30421 attatcagtc ggaaagtctg gtgaatgaaa tttatcgcgg ggaagacagt attgtccgcc
    30481 attggctgac agcgccgtgg agtatggacg gctggcggct ggatgtggtg catatgctgg
    30541 gggaggcggg tggggcgcgc aataatatgc agcacgttgc tgggatcacc gaagcggcga
    30601 aagaaaccca gccggaagcg tatattgtcg gcgaacattt tggcgatgca cggcaatggt
    30661 tacaggccga tgtggaagat gccgccatga actatcgtgg cttcacattc ccgttgtggg
    30721 gatttcttgc caataccgat atctcttacg atccgcagca aattgatgcc caaacctgta
    30781 tggcctggat ggataattac cgcgcagggc tttctcatca acaacaatta cgtatgttta
    30841 atcagctcga cagccacgat actgcgcgat ttaaaacgct gctcggtcgg gatattgcgc
    30901 gcctgccgct ggcggtggtc tggctgttca cctggcctgg tgtaccgtgc atttattacg
    30961 gtgatgaagt gggactggat ggtaaaaacg atccgttttg ccgtaaaccg ttcccctggc
    31021 aggtggaaaa gcaggatacg gcgttattcg cgctgtaccg gcgaatgatt gtgctgcgta
    31081 agaaaagtca ggcgctgcgt cgtggcggct gtcaggtgct gtatgcggaa gataacgtag
    31141 tggtatttgt ccgcgtgctg aatcagcaac gtgtactggt ggcaatcaac cgtggcgagg
    31201 cctgtgaagt ggtgctaccc gcgtcaccgt ttctcaatgc cgtgcaatgg caatgcaaag
    31261 aagggcatgg gcaactgact gacgggattc tggctttgcc tgccatttcg gctacggtat
    31321 ggatgaacta acgttttata acgccttgtg tgacgcctgc gccatcatcc gcggataaaa
    31381 ctgccagaag cgggttccga gggcggtata atgggcgttt aaatcgtacc aggagtcacg
    31441 cagggcatcc agacgtgggc gacggcttgc catgccgttt aacacgttct ggatgaaatc
    31501 catatcgcga tagcgcacca gccactgctc tgaccacaag taattgttta gattgataaa
    31561 acgtggcggt gagtccggca aaatcgtcat cacttgcttg cgggcataac agataaattc
    31621 ctgtagcgga aagtccggcg acagctgcga ccagtggcgg gaaagaaagt gatcccacat
    31681 gacatccagc gtaataggcg caacgcggcg cgtttcacga cgaaaccact cccgtgcttc
    31741 gcggacttcc ggcagattgt cagtcaatac gtcgatacgt cgatgcatat gaatgccagc
    31801 cacgacgtcg ggcggaaaac tttcttcagg atttccgcgt acgaaatcag ccagtaaatt
    31861 gccggaaagc gagctttccg cgagatgggc taaatgcagg tgagctaaaa aattcatcga
    31921 ttatattcta tccaaagggg ggtaaaggtt gcagggagag cgccccggca ctagactacc
    31981 cgcctcttat tttagtctga gtcagtgtca tgcgcgttac cgatttcttc tttgaattgc
    32041 ccgaatccct gattgcccac taccccatgc ctgaacgcag tagctgtcgt ttactgtcgc
    32101 tggacgggcc tacgggcgcg ctgacgcacg gtactttcac cgatttactc gataagctca
    32161 accccggcga tcttctggtt tttaataata ctcgcgtgat cccggcgcgc ctgtttgggc
    32221 gtaaagccag cggcggcaag attgaagtgc tggttgagcg gatgctcgac gacaaacgca
    32281 ttcttgcgca tattcgcgcc tcgaaagcgc caaaacctgg cgcagaactg ctgctgggcg
    32341 atgacgaaag tattaacgca acaatgaccg cgcgccacgg cgcactgttt gaagtcgaat
    32401 tcaatgatga tcgttcggta ctggatatcc tcaacagtat cgggcatatg ccgctgccgc
    32461 cgtatatcga ccgtccggac gaagacgctg accgcgaact ttatcaaacc gtttatagcg
    32521 aaaaaccggg cgcggttgca gccccgaccg ctggtctgca ttttgacgag cctttgctgg
    32581 aaaaattgcg cgccaaaggc gtggagatgg cgtttgtgac gttgcacgtt ggtgcgggca
    32641 ccttccagcc ggtgcgcgtc gacaccattg aagatcacat catgcactcg gaatacgctg
    32701 aagtaccgca ggatgtggta gacgcggtac tggcggcgaa agcgcgcggt aaccgggtga
    32761 ttgcggttgg caccacttca gtacgttcgc tggaaagcgc ggctcaggca gcgaaaaacg
    32821 atctcattga accgttcttc gacgataccc aaatctttat ctatccgggc ttccagtaca
    32881 aagtggtcga tgcgctggtg acgaacttcc acttgccaga gtcgacgctg attatgctgg
    32941 tttcggcctt tgccggttat caacacacca tgaacgccta taaagcagcg gtagaagaga
    33001 aatatcgctt ttttagttac ggtgatgcga tgtttattac gtacaatccg caggcaatta
    33061 atgagcgcgt cggggagtaa ttccgcggcg ctggtttaaa acgttggact gtttttctga
    33121 cgtagtggag aaaaaatgaa atttgaactg gacaccaccg acggtcgcgc acgccgtggc
    33181 cgcctggtct ttgatcgtgg cgtagtggaa acgccttgtt ttatgcctgt tggcacctac
    33241 ggcaccgtaa aagggatgac gccggaagaa gttgaagcca ctggcgcgca aattatcctc
    33301 ggcaacacct tccacctgtg gctgcgcccg ggccaggaaa tcatgaaact gcacggcgat
    33361 ctgcacgatt ttatgcagtg gaaggggccg atcctcaccg actccggggg cttccaggtc
    33421 ttcagccttg gcgatattcg taaaatcacc gaacagggcg tgcacttccg taacccgatc
    33481 aacggcgatc caattttcct cgatcctgaa aaatcaatgg agattcagta cgatcttggt
    33541 tcggatatcg tcatgatctt tgatgagtgt acgccgtatc ctgctgactg ggattacgca
    33601 aaacgctcca tggagatgtc tctgcgttgg gcgaagcgta gccgtgagcg ttttgacagt
    33661 ctcggaaaca aaaatgcgct gtttggtatc attcagggca gcgtttacga agatttacgt
    33721 gatatttctg ttaaaggtct ggtagatatc ggttttgatg gctacgctgt cggcggtctg
    33781 gctgtgggtg agccgaaagc agatatgcac cgcattctgg agcatgtatg cccgcaaatt
    33841 ccggcagaca aaccgcgtta cctgatgggc gttggtaaac cagaagacct ggttgaaggc
    33901 gtacgtcgtg gtatcgatat gtttgactgc gtaatgccaa cccgcaacgc ccgaaatggt
    33961 catttgttcg tgaccgatgg cgtggtgaaa atccgcaatg cgaagtataa gagcgatact
    34021 ggcccactcg atcctgagtg tgattgctac acctgtcgca attattcacg cgcttacttg
    34081 catcatcttg accgttgcaa cgaaatatta ggcgcgcgac tcaacaccat tcataacctt
    34141 cgttactacc agcgtttgat ggcgggttta cgcaaggcta ttgaagaggg taaattagag
    34201 agcttcgtaa ctgattttta ccagcgtcag gggcgagaag taccaccttt gaacgttgat
    34261 taatattaat aatgagggaa atttaatgag cttttttatt tctgatgcgg tagcggcaac
    34321 gggtgcaccg gcgcaaggta gcccgatgtc tttgattttg atgctggtgg tattcggtct
    34381 gattttctat ttcatgatcc tgcgtccaca gcagaagcgc accaaagaac acaaaaagct
    34441 gatggactcc attgccaaag gtgatgaagt tctgacgaac ggtggcctgg ttggtcgcgt
    34501 aaccaaagta gcggaaaacg gctacattgc tatcgcgctg aatgacacca ctgaagtagt
    34561 tattaaacgt gacttcgtag ctgccgtcct gccgaaaggc accatgaagg cgctgtaatt
    34621 aaaatttttc cctaagggaa ttgccgtgtt aaaccgttat cctttgtgga agtacgtcat
    34681 gctgatcgtg gtgattgtca tcggtctgct gtatgcgctt cccaacctgt ttggtgagga
    34741 tccggctgtt cagatcactg gtgcgcgcgg agtcgccgcc agtgagcaaa cgctgatcca
    34801 ggtccagaaa acgttacaag aagaaaaaat aactgctaag tctgtggcac tggaagaggg
    34861 cgctattctt gcgcgcttcg actccactga cacccagctg cgcgctcgtg aagcattaat
    34921 gggcgttatg ggtgacaaat acgtcgtggc gcttaacctt gccccggcaa cgccgcgctg
    34981 gctggcagct attcacgctg agccgatgaa gctcggcctt gacctgcgtg gcggcgttca
    35041 cttcctgatg gaagtggata tggacaccgc gcttggcaaa ctccaggaac aaaatatcga
    35101 tagcctgcgc agcgatctgc gtgaaaaggg catcccgtat accaccgttc gtaaagaaaa
    35161 caactacggc ctgagcatta ctttccgcga tgctaaagct cgtgatgaag ccattgcgta
    35221 tctgagcaag cgccatccgg acctggtgat tagtagccag ggcagcaacc agctgcgtgc
    35281 ggtaatgagc gatgctcgtc tgagtgaagc gcgtgaatat gcggtgcagc agaacatcaa
    35341 tatcctgcgt aaccgtgtaa accaacttgg cgtagcggag ccggtggttc agcgtcaggg
    35401 tgctgaccgt atcgttgttg aactgccagg tattcaggac actgcgcgtg cgaaagagat
    35461 tctgggtgcg acggcaacgc tggaattccg tctggtaaac accaacgttg accaggccgc
    35521 tgcggcatcc ggtcgcgtac cgggcgactc tgaagtgaaa cagacccgcg aaggtcagcc
    35581 agttgtgctg tacaaacgcg taattctgac cggtgaccat atcaccgact ccacttccag
    35641 ccaggacgaa tacaaccaac cgcaggttaa catctcgctc gatagcgctg gtggtaacat
    35701 catgtctaac ttcactaagg acaacatcgg caaaccgatg gcaaccctgt ttgtggagta
    35761 caaagacagc ggtaagaaag atgcaaatgg tcgtgcggtt ctggtgaaac aggaagaggt
    35821 gattaacatc gccaacatcc agtctcgtct gggtaacagc ttccgtatca ccggcatcaa
    35881 caacccgaac gaagcccgtc agctgtcact gctgctgcgt gccggtgcgt tgatcgcgcc
    35941 gattcagatt gttgaagaac gtaccattgg cccaaccctg ggtatgcaga acattgaaca
    36001 ggggctggag gcttgccttg ccggtctgct ggtttctatt ctgttcatga tcatcttcta
    36061 taagaagttt ggtctgattg cgaccagtgc tctgattgcc aacttgatct taatcgtcgg
    36121 cattatgtcg ctgttgccag gcgcaacgct gagtatgcca ggtatcgcgg gtatcgtctt
    36181 aacccttgcg gtggcggtcg atgcgaacgt actgatcaac gaacgtatta aagaagagtt
    36241 gagcaacgga cgtactgttc aacaggcaat tgatgaaggt tatcgtggcg cattcagttc
    36301 tatcttcgat gcgaacatca ccacgctgat taaagtcatc atcctgtacg cagtgggtac
    36361 cggggcaatt aaagggttcg cgattactac cggtatcggt gtggcgacgt cgatgtttac
    36421 cgcgattgtc ggtacgcgtg ccatcgtaaa cctgctatat ggcggcaagc gcgtcaagaa
    36481 gctgtcaatc tgaggagtgc gatgtggcac aggaatatac tgttgaacaa ctaaaccacg
    36541 gccgtaaagt ctatgacttt atgcgctggg actactgggc tttcggcatc tctggtctgc
    36601 tgttaatcgc cgctatcgtt attatgggcg tgcgcggctt taactggggg ctggatttca
    36661 ccggtggtac ggttatcgaa attacgctcg aaaaaccggc tgaaattgac gtaatgcgtg
    36721 atgcattgca aaaagccggt tttgaagagc caatgctgca aaactttggt agcagccacg
    36781 acatcatggt ccgtatgccg cctgctgaag gcgaaaccgg cggtcaggtg ctgggcagcc
    36841 aggttctgaa ggtgattaac gaatccacca atcagaatgc agcagtgaag cgtattgagt
    36901 tcgtcggtcc gagcgtgggg gcagacctta cgcaaaccgg tgcgatggcg ttgatggcgg
    36961 cgctgctgtc tatccttgtg tacgtaggtt ttcgctttga gtggcgactg gcggcagggg
    37021 ttgttattgc gctggcgcac gacgttatca ttacgctggg tattttgtcg ttattccata
    37081 tcgagattga cctgaccatt gtggcatcgt tgatgtcggt tatcggttac tcgcttaacg
    37141 acagtatcgt ggtatcggac cgtattcgtg aaaacttccg caagatccgt cgcggtacgc
    37201 cttacgaaat ctttaacgtg tccttgaccc agacgctgca ccgtaccttg atcacatccg
    37261 gtactacctt gatggttatc ctgatgctgt acctcttcgg tggtccggta ctggaaggct
    37321 tctcgctgac catgcttatc ggtgtttcta tcggtactgc atcttccatc tatgtggcat
    37381 ctgcgttggc tctgaaactg ggtatgaagc gcgaacacat gttgcagcag aaagtggaaa
    37441 aagaaggggc ggatcagccg tcaattctgc cgtaatcaag ttcccgttga tgttgaaaat
    37501 cccggtcaga agatcgggat tttttttgat gtatggagat tgcgaaaatc cccgcatctt
    37561 gggaaactgc gcgtaaccct acatttcatc caggtaactc ttcatggcta tcatcccaaa
    37621 aaactatgcg cggttagaaa gcggctatcg cgaaaaagca ttaaaaatat atccgtgggt
    37681 ctgcggtcgc tgttcccgcg agtttgttta ttccaacctg cgtgaactta ccgttcacca
    37741 cattgatcac gaccatacca ataacccgga agatggcagt aactgggaat tgctttgtct
    37801 ctattgccac gatcatgagc attcgaaata taccgaagcg gatcagtatg gtacgaccgt
    37861 tatcgcaggg gaagatgcgc agaaagatgt cggtgaagcg aagtacaacc cattcgctga
    37921 cctgaaagcg atgatgaaca agaagaagtg attaaaacgt aaaattgcct gatgcgctac
    37981 gcttatcagg cctacgttat ttcagcaata tattgaattt acgtgctttt gtgggccgga
    38041 caaagcgttt acgccgcatc cggcatgaac aaagctcacg ttgttaacaa tcagaaatgc
    38101 cgggaataaa tcccggcatt ttcataatca gaagttgtaa cctactacca ggtaaccacc
    38161 ccagccggta gagcgaacgt tgaagttgcc gttgccgaag ttcagttctg catcgtcgtt
    38221 ccactgacca ccgtcgtgcc agtaacgagc tacgacagag tagtgccagt gatcgtagtt
    38281 cagagccaga atatggctgg aagcgataga gttattagta cgggtcttaa taccgttgat
    38341 tgcgttaccg ctgtcatccc ctaaatcgga accccagtcg aagttggtga agccgatgta
    38401 gctcagctga ccgccccaca gatcggtaat cggcacaaag tatttaattt tgaaacggta
    38461 accgtcccac tcgttttcgt tcgctgcgcc atagttctgc cactggtatt tcgcatagac
    38521 gttcatggac aggctcatcg gcagaccagt atcgatgtcg gtacccagac ccatgtacca
    38581 ggtgctctgg cgaccatttt tattacgacc catgtcgtaa atgtagttgt tcgcgaagta
    38641 ccactctttg aacggaccga agttaaggtc agtattggtc agcttgtcga tggagaaacg
    38701 tggttcgatt tccataaaca gcggagaacc gtggttccag atacctttag catcggagtt
    38761 accgccgaag aataccggcg catccgcata accatagaag tcgaaccagt cttttttagc
    38821 gaatgcttcg tactcaaggt aggtatcgtt gcggatctgc ggtccgaaac gggtgtgata
    38881 gcttccgaca acgttaacgc tctggtgcca ccagtcggaa agatactgcg gtttgtcgtt
    38941 ttcagctgcg ttgacagtaa aagacgaaga gagcgccagt accgcaccgg ctgccagtaa
    39001 tgtttttttc atatgtatgc cactgtttga aaatcccttg cgggagtgaa aaaggcgcaa
    39061 attgcgtttc taaatatttc gtatacagag gagcctatta taaaaatcat tgctcacaaa
    39121 aatatgtttc gtttcacagt tctatcattt acgtaatcga ttgcgttcac gtttacacac
    39181 attcgggcgg ggattgtact gactttcgcc cttgttgcaa acttttacta tcaatccagg
    39241 tgatgtaagc agagtaacaa aatgacgggg aaagatgttg cgggacgcac aaaatcgtcc
    39301 cgaaatacgt ttactgcata acaggttgaa tgtcgtggat acgtacaaag cctaactgat
    39361 ccacagaaat cccctttaat tgtaacggaa tatcaacatc gctgggggcg aggacgctgg
    39421 caggggcatt aatcaattga ttctgcacat tgatttcctg aaagttgtct attgttccct
    39481 gaatttgccc atattcaaca gttccgctga atgcgggcaa gggatcattc gactcaccct
    39541 gaatacgtag tgtaagggtg gtgccatctg tatcaggcgt aatattcacc agcgacatac
    39601 gtaaagtacc gatctggctt tccagtcttg ccggtgtttt cgctccaggc agaaggtaac
    39661 ccccactgct ggattttgca ttcagcctgt tttgctgagt gattttgact gtttcttgat
    39721 tgagttgcgt catctcctgg ttcagcgtgc tgacgctgtg tttcatctgt cgcacttcac
    39781 tttgctgcac acaggcgcta aggctgaaca ggcttcccag caggagcagt cgaaaaacgt
    39841 tagtattcat aggtcgtccc tttctcgtcg caattagcct aatggtagag ggtaaaagca
    39901 gcgaaagcat cgatccttta tctcaaaagc gttgcgcctt tgttgtatcg tcagttcagg
    39961 gtaaaataga tttccgttaa ccacctggtc aggacgccgt atgcattgcc cattctgttt
    40021 cgccgtggac actaaggtaa ttgactctcg tctcgtgggc gagggttcat ccgtacgccg
    40081 ccgtcggcag tgtctggtgt gtaatgaacg tttcaccacc tttgaagtgg cggagctggt
    40141 tatgccgcgt gttgtaaaaa gcaacgacgt gcgtgaaccg tttaatgaag agaaattgcg
    40201 tagcggaatg ctgcgggcgc tggaaaaacg tccggtgagt tccgatgacg ttgaaatggc
    40261 aatcaatcat attaaatcgc agctgcgcgc caccggtgag cgcgaagtac cgagcaagat
    40321 gattggcaat ctggtgatgg agcaattgaa aaagctcgat aaagtcgcct atatccgttt
    40381 tgcctctgtc taccgcagtt tcgaagatat caaagaattt ggcgaagaga tcgcgcgcct
    40441 ggaggactaa gccgtgcagg acgagtatta catggcgcgg gcgctaaagc tggcgcaacg
    40501 aggacgtttt accacccacc ccaacccaaa tgtcgggtgc gtcattgtca aagatggcga
    40561 aattgtcggt gaaggttacc atcaacgagc gggtgaacca catgccgaag tacacgcgtt
    40621 gcgtatggcg ggtgaaaaag ccaaaggtgc aacggcgtat gtgacactcg aaccttgcag
    40681 ccatcacggc cgtacgccgc cttgctgtga tgctttaatt gccgcaggtg tggcgcgcgt
    40741 ggttgcctcg atgcaagatc ctaacccgca ggtcgctggg cgtggacttt accgtctgca
    40801 acaggctggc attgacgtca gccacggcct gatgatgagt gaagccgagc aattgaataa
    40861 aggctttctc aagcggatgc gcaccggctt tccttatatt cagttaaaac ttggcgcatc
    40921 gcttgatggt cgcacggcga tggcgagcgg cgaaagccag tggatcactt cgccccaggc
    40981 gcggcgtgat gtacaacgac tgcgcgcgca aagtcatgcc attttaacca gcagcgccac
    41041 ggtgctggcg gatgatcctg ccttaacggt gcgttggtcc gaactggatg aacaaactca
    41101 ggcgctctac ccgcaacaaa atctccgcca gccggtacgt attgtgattg atagccaaaa
    41161 tcgcgtgacg ccggaacatc acattgtgca gcagcccggc gaaacctggt tcgcgcgtac
    41221 gcaggaagat tctcgtgagt ggccggaaac ggtgcgtacc ttgctgattc cagagcataa
    41281 aggtcatctg gatctggttg tactgatgat gcaactgggt aaacagcaga ttaacagcat
    41341 ctggggggaa gcggggccaa cgctcgctgg cgcattgctg caggcgggtt tagtcgatga
    41401 gctgattgtc tatatcgcac ctaaactatt aggcagcgac gcccgtggat tatgcaccct
    41461 gccagggctt gagaaattag ccgacgcccc ccaatttaaa ttcaaagaga tacgtcatgt
    41521 aggcccggat gtttgcctgc atttagtggg tgcatgatct cccggctcga aagggaagca
    41581 gcgcacgaaa tattatgcta aaatccgccc ccctgcgggg ccatactcga acccgaagga
    41641 agaaaatgaa cattattgaa gctaacgttg ctaccccgga cgctcgcgtc gccatcacca
    41701 tcgcgcgttt caacaacttt atcaatgaca gcctgctgga aggtgcaatt gacgcactga
    41761 aacgtatcgg tcaggtaaaa gatgaaaaca ttaccgttgt ttgggtgcct ggtgcctatg
    41821 agctgccgct ggcggcgggt gcactggcta aaaccggtaa atacgacgcg gtgattgcgc
    41881 tgggtacggt tattcgtggt ggcactgccc attttgaata tgtcgctggt ggtgcaagca
    41941 acggcctggc gcatgttgcc caggacagcg aaattccggt tgcttttggg gttctgacca
    42001 ctgaaagcat tgaacaagcg atcgaacgtg ctggcaccaa agctggcaac aaaggtgcag
    42061 aagctgcact gaccgcgctt gaaatgatta atgtattgaa agccatcaag gcctgaaatt
    42121 agtaagggga aatccgtgaa acctgctgct cgtcgccgcg ctcgtgagtg tgccgtccag
    42181 gcgctctact cctggcagtt gtcccagaac gacatcgctg atgttgaata ccagttcctg
    42241 gctgaacagg atgtaaaaga cgttgacgtc ctgtacttcc gtgagctgct ggccggggtg
    42301 gcgactaata ccgcatacct cgacggactg atgaagccat acctgtcccg tctgctggaa
    42361 gaactgggac aggtagaaaa agcagtactg cgcattgcgc tgtacgaact gtctaaacgt
    42421 agcgatgtgc catacaaagt ggccattaac gaagcgatcg aactggcgaa atcgttcggc
    42481 gcagaagaca gccataagtt cgtcaacggt gtactcgata aagcagcacc tgtgattcgc
    42541 cctaacaaaa agtaatatcc aggccggtag attcacggaa gaccgttcca tgatcgccgg
    42601 ccttttcttt tttacctgct gaggcataac gtatggcatg tggcgagttc tccctgattg
    42661 cccgttattt tgaccgtgta agaagttctc gtcttgatgt cgaactgggc atcggcgacg
    42721 attgcgcact tctcaatatc cccgagaaac agaccctggc gatcagcact gatacgctgg
    42781 tggcgggcaa ccacttcctc cctgatatcg atcctgctga tctggcgtat aaagcactgg
    42841 cggtgaacct aagcgatctg gcagcgatgg gggccgatcc ggcctggctg acgctggcat
    42901 taaccttgcc ggacgtagac gaagcgtggc ttgagtcctt cagcgacagt ttgtttgatc
    42961 ttctcaatta ttacgatatg caactcattg gcggcgatac cacgcgtggg ccattatcaa
    43021 tgacgttggg tatccacggc tttgttccga tgggacgagc cttaacgcgc tctggggcga
    43081 aaccgggtga ctggatctat gtgaccggta caccgggcga tagcgccgcc gggctggcga
    43141 ttttgcaaaa ccgtttgcag gttgccgatg ctaaagatgc ggactacttg atcaaacgtc
    43201 atctccgtcc atcgccgcgt attttacagg ggcaggcact gcgcgatctg gcaaattcag
    43261 ctatcgatct ctctgacggt ctgatttccg atctcgggca tatcgtgaaa gccagcgact
    43321 gcggcgtacg tattgacctg gcattgctgc cgttttctga tgcgctttct cgccatgttg
    43381 aaccggaaca ggcgctgcgc tgggcgctct ctggcggtga agattacgag ttgtgtttca
    43441 cggtgccgga actgaaccgt ggagcgctgg atgtggctct cggacacctg ggcgtaccgt
    43501 ttacctgtat cgggcaaatg accgccgata tcgaagggct ttgttttatt cgtgacggtg
    43561 aacctgttac gttagactgg aaaggatatg accattttgc cacgccataa agatgtcgcg
    43621 aaaagtcgcc tgaagatgag taatccgtgg catctacttg ctgtcggatt cggaagtgga
    43681 ttaagcccga tcgttcctgg gacgatgggc tcgctggcag cgattccgtt ctggtatctg
    43741 atgacctttt tgccctggca gctctactcg ctggtggtga tgctggggat ctgtatcggc
    43801 gtctatcttt gtcatcagac cgcaaaagat atgggcgtgc acgatcacgg cagcattgtc
    43861 tgggacgaat ttattggtat gtggatcacg ctcatggcgc tgccgaccaa tgactggcag
    43921 tgggttgccg ccgggtttgt gattttccgt attctggata tgtggaaacc gtggccgatc
    43981 cgctggtttg atcgcaatgt gcatggcggc atggggatca tgatagatga tattgtcgcc
    44041 ggggtgatct ccgctggcat cctgtacttt attggtcatc actggccgct gggcattctg
    44101 tcgtagttgt gcaccgatgc ctgatgtgac gcttgttgcg tctcatcagg cctggactct
    44161 tatttaaatc ctactaccgg atgcggttta tacggcgttt ccagttcggc aatctgttcc
    44221 ggcttcaaag tgatatccac cgcgttcaat agctcatcaa gctgttcttc ccgcgatgta
    44281 ccgataatcg gtgcggcaat gcccggttta ctcaacaacc aagccagcgc aacttgtgct
    44341 cgtgttgcac cgagttcttc actgacgccc gttaaccgct ctgcgatctg cgcgtcattt
    44401 tcatcgcttt ctttatagag atttttcccc acctcatcag acaccagtcg tgcggtagtt
    44461 tctccccacg gacgcgtcag tcgcccccgc gccagcgggc tccacggaat caccgccacg
    44521 ccctcctgat aaaacagtgg cagcatctcg cgctcttctt cgcgataaat cagattgtag
    44581 tgatcctgca tactgacaaa ctgcgcccag ccgtgctgtt tttggagttc cagtgcctga
    44641 gcaaactgcg aagcgtgcat tgatgacgcg ccgatataac gcgctttccc ggcttttacc
    44701 acgtcgttca gggcttccag cgtctcttcg atcggcgtgt tgtaatccca gcgatgaatt
    44761 tgcaggatat cgacataatc catgccgaga cgtcgcaggc tgtcgtcgat agagcgcaaa
    44821 atttgtgcac gggataatcc ttccggtaaa tcaccaacgc gatggaacac tttggtggca
    44881 acgaccacgt cttcacgacg ggcgaaatcc cgcagtgcgc gaccaacgat ctcttcgctg
    44941 ctaccatcgg aatagctatt ggcggtatca aagaaattta tgccgccttc cagtgcgcgt
    45001 ttaattatgg gacggctgct ctcttccggc agtgtccatg cgtgattacc acgatctggc
    45061 tcgccaaagg tcatacagcc gaggcaaagt cgggaaacgc gaaggtcggt ttttcctaag
    45121 gggttgtatt gcatgctgcc actcctgcca tgaataattt cttaagcata gcaggagtgg
    45181 agtagggatt atgccagcca ggccttgatt ttggcttcca taccggcggc atcgaggccg
    45241 agttcggcgc gcatttcttc ctgcgttcct tgcggaataa agaagtcagg caggccaatg
    45301 ttcagcacgg ggactggttt acgatgggcc attagcactt cgttcactcc gctgcctgca
    45361 ccgcccataa tggcgttttc ttctacggta accagtgctt catgactggc ggccatttcc
    45421 agaattaacg cttcatcaag cggtttcaca aaacgcatat cgaccagcgt ggcgttcagc
    45481 gattcggcga ctttcgccgc ttctggcatc agcgtaccaa agttaaggat cgccagtttc
    45541 tcgccacgac gcttcacaat gcctttgcca attggtagtt tttccagcgg cgtcagttcc
    45601 acgccgaccg cgttgccacg cgggtagcgc accgctgacg ggccatcgtt atagtgatag
    45661 ccggtataga gcatctggcg acattcgttt tcatcgctcg gggtcataat gaccatttcc
    45721 ggtatgcagc gcaggtaaga gagatcaaaa gcaccctgat gggtttgacc gtcagcacca
    45781 acaatgcccg cgcggtcgat ggcgaacagg accggaagct tttgaattgc cacgtcatgc
    45841 agcacctgat cataggcgcg ttgcaggaaa gtggagtaga tcgcgacaat gggtttgtac
    45901 ccaccaatcg ccagaccggc ggcaaaggtc actgcgtgtt gctcggcaat tgccacgtcg
    45961 aagtagcgat ccgggaattt acgagaaaac tcgaccatgc cggaaccttc acgcatcgcc
    46021 ggagtaatcg ccatcagctt gttatctttc gccgccgttt cgcacaacca gtcgccaaag
    46081 atttttgaat agctcggtaa accgccgcta cttttcggca aacaaccgct ggagggatca
    46141 aatttaggca cggcgtggaa agtgattggg tctttttctg ccggttcata accacgacct
    46201 tttttggtca tgatatgcag gaactgcggg cctttcaggt cacgcatgtt cttcagcgtg
    46261 gtgataagcc ccagcacatc gtgaccgtca accgggccga tgtagttaaa gcccagctct
    46321 tcaaacaacg tgccagggac taccatgcct ttaatatgtt cttcggtacg tttgagcagc
    46381 tctttaatgg gtggcacgcc agagaaaact tttttcccgc cttcgcgcag tgaagagtaa
    46441 agcttaccgg aaagcagctg cgccagatgg ttgttgagcg cgccgacatt ttcggaaatc
    46501 gacatttcgt tgtcgttgag gaccaccagc atatcaggac ggatatcgcc cgcgtgattc
    46561 atcgcttcaa acgccatgcc tgcggtaatc gcgccatcgc cgattacaca gacggtacgg
    46621 cgatttttgc cttctttctc ggcagcaacc gcaataccaa ttccggcact gatggaggtt
    46681 gatgaatgcc cgacgcttaa tacgtcatat tcgctttcgc cgcgccacgg gaacgggtgc
    46741 aggccgcctt tctgacggat ggtgccgatt ttgtcgcggc gtccggtcaa aattttatgc
    46801 ggataagcct gatgccccac atcccaaatc aattggtcaa acggggtgtt gtagacatag
    46861 tgcagcgcca cggtcagttc gaccgtgccc agcccggagg cgaagtgccc gctggaacgg
    46921 ctcacgctgt cgagtaaata gcggcgcagt tcgtcgcaga gtttcggtaa actctctttc
    46981 ggcaacagtc gtaactcctg ggtggagtcg accagtgcca gggtcgggta tttggcaata
    47041 tcaaaactca tcaggggcct attaatactt attgtttatt tattacgctg gatgatgtag
    47101 tccgctagcg cttccagtgc cgaggtatcg ggtgactgtt cagccagttg tttcagcgac
    47161 tgacgggcat cgtcgatcag atcccgggct ttcttccggg cttgctcaag acccagaagt
    47221 gcagggtagg tacttttacc aagttgctgg tcggcaccct ggcgttttcc caacgttgca
    47281 gtatctccta ccacatccag gatgtcatcc tgaacctgga aggcaaggcc gatgctctct
    47341 gcgtacttgt cgagtactgg cagagcacga cgccctttat ctccggcgct taatgcacca
    47401 agacgaacgg cggcgcgaat caatgcgccg gttttatgac gatgaatacg ctcaagcgcg
    47461 tccagaggta cgtgtttgcc ttccgcgtct aaatctaatg cctgaccacc gcacattccg
    47521 gcaataccgc tggcgctcgc cagttcagaa atcatcgaaa ttctgtcgcg atccgacact
    47581 tccggcatat cagcatcgct taaaatcgag aacgccagcg tttgtaaagc gtcgccagcg
    47641 agaatcgcgt ttgcttcgcc aaacttcaca tggcaggtcg gcaaaccgcg acgcagatcg
    47701 tcatcatcca tcgccggtaa atcatcatga attaatgagt aagcgtggat acactctacg
    47761 gcggcagcgg gtgcgtccag cgtgtttgtg ctaacgccaa acatatgacc ggtggcataa
    47821 accaggaagg gtcgcagacg cttaccacct aataatgcac catactgcat ggtttcgacc
    47881 acgggagtgt tctgaaaggg cagtggggcg ataaaacggc tcagcgcctg gttggcctgc
    47941 ttaacgcagg cttcgagttg ctgcggaaag tccattactc attgtccggt gtaaaagggg
    48001 ttagagaggc gtcttcattg tcagacagca gaatttgtac gcgctgttcg gcttgttgta
    48061 atttggcctg cccctgacgt gccagctgca cgccgcgttc gaactcgttc agcgcctctt
    48121 ccagcggcag gtcgccactt tccagacggg ttacaatctg ttccagctcg ctcagcgcct
    48181 tttcaaagct ggcgggagcc tcatttttct tcggcataat gaatgtctga ctctcaatat
    48241 ttttcgcccc gtcatggtaa cggactcagg gcaaatagca aataacgcgc aatggtaagg
    48301 tgatgtgcgc agcaaagcga tgttagtggt atacttccgc gcctggatgc agccgcaggt
    48361 gtgggctgct gtatttttcc ctatacaagt cgcttaaggc ttgccaacga accattgccg
    48421 ccatgaagtt tatcattaaa ttgttcccgg aaatcaccat caaaagccaa tctgtgcgct
    48481 tgcgctttat aaaaatcctt accgggaaca ttcgtaacgt tttaaagcac tatgatgaga
    48541 cgctcgctgt cgtccgccac tgggataaca tcgaagttcg tgcaaaagat gaaaaccagc
    48601 gtctggctat tcgcgacgcc ctgacccgta ttccgggtat ccaccatatt ctcgaagtcg
    48661 aagacgtgcc gtttaccgac atgcacgata tttttgagaa agcgttggtt cagtatcgcg
    48721 atcagctgga aggcaaaacc ttctgcgtac gcgtgaagcg ccgtggcaaa catgatttta
    48781 gctcgattga tgtggaacgt tacgtcggcg gcggtttaaa tcagcatatt gagtccgcgc
    48841 gcgtgaagct gaccaatccg gaagtgactg tccatctgga agtggaagac gatcgtctcc
    48901 tgctgattaa aggccgctac gaaggtattg gcggtttccc gatcggcacc caggaagatg
    48961 tgctgtcgct catttccggt ggtttcgact ccggtgtttc cagttatatg ttgatgcgtc
    49021 gcggctgccg cgtgcattac tgtttcttta accttggcgg cgcggcgcat gaaattggcg
    49081 tgcgtcaggt ggcgcattat ctgtggaacc gttttggcag ctcccaccgc gtgcgttttg
    49141 tcgctattaa tttcgaaccg gtcgtcgggg aaattctcga gaaaatcgac gacggtcaga
    49201 tgggcgttat cctcaaacgt atgatggtgc gtgccgcgtc taaagtggct gaacgttacg
    49261 gcgtacaggc gctggtcacc ggcgaagcgc tcggccaggt gtccagtcag acgctgacca
    49321 acctgcgcct gattgataac gtctccgata cgctgatcct gcgtccgctg atttcttacg
    49381 acaaagagca catcatcaac ctggctcgcc agattggcac cgaagacttt gctcgcacga
    49441 tgccggaata ttgcggtgtt atctccaaaa gcccgacggt gaaagcggtt aagtcgaaga
    49501 ttgaagcgga agaagagaaa ttcgacttca gcattctcga taaagtggtt gaagaagcga
    49561 ataacgttga tatccgcgaa atcgccctgc agaccgagca ggaagtggtg gaagtggaaa
    49621 ccgtcaatgg cttcggcccg aacgacgtga tcctcgatat ccgttctatc gatgaacagg
    49681 aagataagcc actgaaagtc gaagggattg atgtagtttc tctgccgttc tataaactga
    49741 gcaccaagtt tggcgatctc gaccagaaca gaacctggct actgtggtgt gagcgcgggg
    49801 tgatgagccg tctgcaggcg ctctatctgc gcgagcaggg ctttaacaat gtgaaggtat
    49861 atcgcccgta atttgtggtt gtaacgtcgc atctggtcag atgcgacgct taccgcatcc
    49921 gacactactc gtaataatta taaatccctg ccgccatcac cagttgtgat gccacttcat
    49981 gggctttctc gcgcccaacc tgcaggtcga taattttcag accaaagtcg atagctgtcc
    50041 ccggcccctg gctggtcagc aattttaccc gtgcatccca gacgacgcgc ttgtcctgcc
    50101 attgttcagc gggaattttg tctttcagcg tcgggaagcc ggtcatatta ccaatcggga
    50161 agatatcgtg cggcaccagc acggtggctg gcgcggcgca aatagccgcg acgatacgcc
    50221 cggaacggtg gaactgttta acggtttcaa ccagcagagt gctatcgcga aaacactctg
    50281 cgcctttaat gccaccaggc agtacgatca cgtcatattc gccatcagcc acctcgacca
    50341 gcggcgcatc cgccagcagc ttcacgccgc gcgagcaggt aattgccagg ttgccatcgc
    50401 tggctacgct ggcagtggtg actttgatac cgccgcgaac caagaggtcg atcgtggtga
    50461 cggcttcagt ctcttcacta ccaggggcga ggcaaaccag tgccgatgcg ctcatattca
    50521 ctctcctttc tttttaccat ttcaaacagg cgggtgtttt ccggtacggc aatcccatgc
    50581 gcgcgggcgc ggcgtaagag aaaaccattg atatagtcga tttcggtgtg gcgcagcgcg
    50641 cggatatcct gcaacatcga cgagatattt tccgctgtgg catcaatcac ctgcatcacg
    50701 taatcacgca aatcttccgc tgaagtatga tgcccttcgc gttcgatcac tgccgcgact
    50761 tcttcgcata tttgcataat ttcttgcgga tgatgacgta attcaccgtt cgggcaattc
    50821 cagatggcgg tcagtggatt aatcacgcag ttgactgcca gcttgcgcca cagctcggcg
    50881 cgaatattgt tatgccaggc aacgtcaggc aacacggttt gtaaaatatc cgccagataa
    50941 ctgtaatcgc catcctgttg ccgtgccgga ccaatatgcg tgataccgtt tgccacatga
    51001 ataatgacat tgccgtcgcg gcgggcggca tgggtggtgg tgcccatcag taatggctgc
    51061 tgaatatttt gcaactcttc gatggtgccc atgccgttgt gaattaacag tattggcgtg
    51121 gttacaggca gtgtggacgc gaggcttttg acggcatcgg aaacctgcca tgctttcagc
    51181 gtcaccagga gcagatcgct ggtggcgaga aaatcgggat cgttggcggt cagcgattcg
    51241 ttaaatatcg aaccatctgt ctcaaccaga ttcgcactac aataaggttg cggtacgcgc
    51301 agccagccct gaacttcatg accctgtttg caaagtgctg taagccataa ttgccctaag
    51361 gcaccgcatc ccaatacggt aattttcatt gttcctcctc acccgcaacc actccgggtg
    51421 ttcaataagg ctatccctta attgtgcatg ctgttgcgac tatgcacaat taagggatac
    51481 gtcctggtgc aggactgtcg gttatttaac tttgcgggta ttatgcttcg catcaaaaat
    51541 gaagggagag gaaaagatgc catctttcga tattgtctct gaagttgatc ttcaggaagc
    51601 acgtaacgcg gtcgataacg cgagccgcga agtggagtcc cgttttgact tccgtaacgt
    51661 tgaagcctca tttgagctga acgacgccag caaaaccatc aaagtgttga gcgagtccga
    51721 tttccaggtc aatcagttgc tggatattct gcgtgccaag ctgctgaagc gcggcattga
    51781 aggcagttca ctggatgtac cggaaaatat cgttcatagc ggtaaaacct ggtttgtgga
    51841 agcgaaactg aagcagggca ttgagagcgc gacccagaaa aaaatcgtca agatgatcaa
    51901 agacagcaaa ctgaaagtgc aggcgcaaat tcagggcgat gagatccgcg tgactggcaa
    51961 atctcgtgat gatttgcagg ctgtcatggc gatggtgcgc ggtggcgatc tgggtcagcc
    52021 gttccagttc aaaaacttcc gcgattaatc gcgacaacct gcgctttgtt catgtcagat
    52081 gcggcatgaa cgcctggttc ggcctacaaa ggcttgtaaa tcaggagctt agtaggcctg
    52141 atgagcgtag cgcagcaggc aatttcgcct cgcttttatg cctggcgaat tgcctgttca
    52201 acatcaaagc gattcgtcac tttgctgtcg attttcacat aagctgaatg ttcttcttct
    52261 gcaatcaaca cttctttgat gccttcagtt tctagcaaac gcacttttaa cgcctcgttt
    52321 gcggcaatgt tcgccgggat ttcaatgcgc aaactgctga catacgccgg ttctttcatg
    52381 gtactggcga ctgccagcca cactgcggcc agcattgcgc cagcgagaaa tacgccctga
    52441 ccgtcaaaca tgccgtcaat ccagccgccc agtgaaccgc caatcgccac gccaagaaac
    52501 tggctggtgg agtaaacacc catcgctgta cctttgtaac ctgctggcga ctctttactg
    52561 ataagtgaag gcagaagggc ttccatcaaa ttaaacgcta caaagaaaag ctgcacgccg
    52621 accaccagtt gccagaactg cgtttgcgcg ttccacaaca caatttccgc aaccacgatc
    52681 aatccgacgc agaagacaaa gacttgcttc attttgcgct taacttcagc gtagataatg
    52741 aaaggcacga ccgagccaaa ggcgattagc attgtcgcca gatagacctt ccagtgttca
    52801 gccgccggga accctgcatc agccagttgt ccgggcaggg caacaaacgt cgacatcagc
    52861 aaaatatgca gacacataat gccaaagttg agtttcagca gccgcggttc cgccagcact
    52921 ttactgaaac tgcctttcac cattccggac tcacgattaa gtacgtgagt gctactgttg
    52981 ggcacaaccc aaatggtcaa cgcaatgccg gtcgttgcca gaatagcgat catccagaac
    53041 agcgcgtgca gcccaagttt gtgagtgatg atcgggccaa gcaccatcgc aatggcaaag
    53101 gtaatgccaa agctcacgcc gataaacgcc attgctttgg tgcggttttg ttcgcgcgtg
    53161 agatcggaaa gcagcgccat aacggcagcg gcaatcgcac cggagccttg tagcgcccgg
    53221 cctagaataa ttccccagat ggagtcagag agcgcagcga taacgctacc ggcggcaaac
    53281 accgccagcc caccgacaat taatggtttg cgaccaatac ggtcggaaag caggccaaac
    53341 ggaatctgaa aaacggcctg agtcagacca taaataccaa tggcaatacc gattaatgct
    53401 tcgctggcac cttgcagagc catgccatac gtggtcagaa ccggcagaac cataaacatg
    53461 cccagcatgc gcaacgagaa tacggtccct aaaccccagg tcgcgcgcct ctcacctggc
    53521 gtcattttat aatcgttcat taccacctct gttttaaatt cgcgactagt gtaaagcggc
    53581 aaacgaatgg ggtaaacatg cggatttttt agtaaatatt tcgagaggga tatgtttcta
    53641 atgctaagaa aaaaggtgcc gtagcacctt tttaatagag aggttttgtt accacacagc
    53701 agccagcagc gtatgcgagt ccggtaccat aaaatcaacg gacatcatca ccgagagggc
    53761 agtgatggcg atgatagaga agccgaacag cttgcgcgcc cagattctgt catcagcaac
    53821 tttataaccg cgcagagcca tacctaacca ccagacgcta accgccgcgg cgaccaccag
    53881 atatttatat ccagcgtaac cgccaagaga gagcatcagc gtggcaacgg caaaggcgat
    53941 gatatacagc gtgatgtgat tcttcgccac cgaaatgcct tttaccactg gcaataccgg
    54001 aatgtttgcc gcctggtaat ccttaaagcg gaaaatggcg atggcatagg agtgaggcat
    54061 ctgccacagg ctgaagatag ccagcaggat cgctgcgccg ctatcgaact cgccggttac
    54121 tgcacagtag ccaatcaccg gcggcgcagc gccggagagc gaaccaatca atgtgccgta
    54181 gactgagtgg cgtttcatgt acaggctata gacgccgaca taaaccacaa agcccatcac
    54241 ccccagccag caggccagcg gattcgcgcc aaaccacagc agcataaagc cagcaatacc
    54301 cagcaaggtg gcgtacacca gcgagacagc aggagagatc aggcctttaa ccagcacccg
    54361 attcttcgtc ctttccatct ttctgtcgat atccctgtcg atgtagttgt taaacacaca
    54421 acccgacgcc acaaccagtg acaccccaac cagcgtgtag ataaacaggg gataatcaat
    54481 gctgcccttt gaggccagca ggaatccccc aatcaccgag atcaggttgc caaagatgat
    54541 gcctggtttc gttacttgca ggtattgctt aaacatcata accgccgctc ttagtgcatc
    54601 atcatgttgt agttgaggtt ccacataatc cagatggagc ctacaaccag gatagcgatg
    54661 attagcacgg tgaagacaaa cgccgtcatg ttccagcctt catctgattt ggtattcatg
    54721 tgcaggaagc acaccagatg caccagaacc tgtaccactg ccattgccag gattgttccc
    54781 agaattacgg ccggagaggc agcccctgtc atcaccatcc agaacggaat caccgtcaga
    54841 atgatcgaca ggataaagcc tgtcatgtag gtttttacgc tgccatggga cgcgccgctg
    54901 tgatcggtag aatgactcat tacatcgccc ccatcagata aacaacagtg aacacacaga
    54961 tccaaaccac atccaggaag tgccagaaca ggctcaggca catgatgcgg gtacggttag
    55021 tgctggtcag gccgcgacga gcgatttgta ccatcagcac cgccatccag ataagaccgg
    55081 aagtgacgtg cagaccgtgc gtgccgacca gcgcaaagaa cgctgacagg aagccgctgc
    55141 gatccggacc catgccgtta acaatcaggt gatggaattc gtagatttcc atcccgataa
    55201 atccggcacc aaacaaacag gtcaacgcca gccaggagat aacctggctt ttgttgtttt
    55261 tgtacatggc gatagccgcc atgccgtagg tgatggagct gaacaacagc aagaaagttt
    55321 caaccagaac gaacggcagt tcgaaaatgt ccttacctgt cgggccgcct gcggtgccgt
    55381 tcaccagaac ggcataggta gcaaacaaga ttgagaacag aatgcagtcg ctcatcaggt
    55441 agatccaaaa tccgaagatt ttggttccgc ctgcatcgtg gtgcccgtgt tcgtgcgcgt
    55501 gggcagtcgc gtgcgtcaaa gtatcagttg ccatttttca gccctgcctt agtaatctca
    55561 tcgaaatgct ggttttccag tttttcgatt tctgccaccg gcacgtagta atccacgtcc
    55621 tcgtcgaagc ttttcacgat ccaggtgatg atcatgcctg cgaagccaac aatcgccagc
    55681 caccagatat gccagatcat ggcgaaaccg aagatggtgg agaaagctgc aatgacgata
    55741 cctgcaccgc tgtttttcgg catatgaatt tcttcatagt ggtcaggctt tttatacgct
    55801 tcgcctttct ctttcatttc ccagaatgca tcacgctcgt gaacgtgcgg cacaacggca
    55861 aagttataga acggaggcgg ggaagagttt gcccactcca gcgtacggcc accccacggg
    55921 tcgccagtca ggtcacggtt ctggtcgcgg tcgcgaatag aaacgtacat ctgaataacg
    55981 aggcagagaa tacccagcgc aatcagtact gcaccgctag ctgcaatcat cagcatggtg
    56041 tggaactgcg ggtcaatctg ctggctcaaa cgacgggtca tgcccatgaa gcccagtgca
    56101 tacagcggca taaaggcaac gaagaagccg atgatccaga accagaacgc gcgtttaccc
    56161 caggtttcat tcagtttgaa accgaacgct ttaggccacc agtaggtcat ccctgcgaag
    56221 cagccgaaga ccacgccgcc gatgataacg ttatggaagt gcgcaatcag gaacaggctg
    56281 ttatgcagaa cgaagtccgc gcccggtact gccagcagca cgccagtcat cccgcccacc
    56341 gagaaagtga cgataaaacc gatggtccac agcatcgcag aatggaacac gatacggccc
    56401 tgatacatgg tgaacagcca gttgaagatc ttcaccccgg tcgggatggc gataatcatt
    56461 gtggtgatac caaagaaggc gtttacgttc gcgcccgcac ccatcgtaaa gaagtggtgc
    56521 agccaaacga tgaacgacag cacggtgata cagacggttg cccataccag cgaggtataa
    56581 ccaaacagac gtttacgcga gaaggttgcc gcaatttcgg agaacacacc gaaaacaggc
    56641 aggatcagga tgtaaacttc cgggtggccc caggcccaaa tcaggttgat gtacatcatc
    56701 atgttgccac ccatatcgtt ggtaaagaaa tgggtgccca gatagcgatc cagggtcaac
    56761 aacgcgacgg taaccgtcag aattgggaag gaagcaataa tcagtacgtt cgcgcacagt
    56821 gatgcccagg taaatactgg catcttgaac atggtcatgc ccggtgcgcg catcttcaga
    56881 atggtaacga agaagttgat accggtaagc gtcgtaccta taccggatag ctggagactc
    56941 catatccagt aatcaacacc gactcccgga ctgtactcta ttcccgatag cggtggatag
    57001 gccagccagc cggtctgcgc aaattcgccc acgccgagag aaacgttaac cagaatcaca
    57061 ccaacaacag taaaccagaa gcttaagttg ttgaggaacg ggaacgcaac gtcacgcgcg
    57121 ccgatctgca gcggaaccac caggttcatc agaccgataa cgaaaggcat cgccacgaag
    57181 aagatcataa tcacgccgtg cgcggtgaag atctgatcgt agtggtgagg tggcaggaag
    57241 cccgcttcgc ccgccgaggc aagagcctgc tggctacgca tcataatggc gtcagcaaaa
    57301 ccacgcagca acatcacaat cgccacgatg atatacatga taccgaggcg tttatggtcg
    57361 acggaggtca gccactcttt ccacaggtag gtccacttac cgaagtaagt gatcaggcca
    57421 acgagcgcca gacctcccaa aataatgcca gcgatcgtaa ccatgacgat aggttcatgg
    57481 aacgggactg catcaagtga taattttccg aacatcttta ttcttcctca acccctttaa
    57541 tgggcggatt ccgcgtggct catgtccatg ccttccatac cttcgtgtgc gctgtgctca
    57601 ccttctggct gggtcatgtc catgctctta ccgtgagcca taaacttgtt aatcacatca
    57661 gcaaacaagt ctggtttcac gttggagaaa tattccacct ggttgtattc gctaggcgcg
    57721 gccagttttt cgaacgcagc catgtcagac atggtgttcg gcgactgctt cgcttttgcg
    57781 acccactggt cgaattcggc gcgatccggt gttgcaatag ctttgaactt catgcctgag
    57841 aagcccgggc cgctgtagct ggcggagata ccgtcataag taccgggttc gttggcgatc
    57901 agatgcaggc gagtctgcat accagccatg gcataaatct ggctacccag acgcggaatg
    57961 aagaaggagt tcatcacgga gttggaggtc actttgaagt acaccggagt gttcgccggg
    58021 aaagcgattt cattcacggt agcaatgccc tgttccgggt agatgaagaa ccatttccag
    58081 tccatggaaa ccacttcgat ggtaatgggc ttctcgtcgt gtgccagcgg cttgctaggc
    58141 tcaagagcgt gagtggtttt ccaggtcagt accgcaagga agatgatgat taagataggt
    58201 accgtccaga ccacagcttc cactttattg gagtgtgacc agttcgggct gtacttagca
    58261 tctttattgc tcgcacgata cttccaggcg aaaccaacag ccatcaagat tgcgggaata
    58321 acgacaatca acatcaggcc aaatgccgtc agtatcagtg aacgttgctc cagaccaatc
    58381 tgtcctttgg gatctaacag cgcagaatta cagccactga gcaatacagt gcctgcaaat
    58441 aatgacaacc atcccaaact tttattgtat ttcctgagtc tcatttaacg acctcaattc
    58501 cacgggatct ggtggcgttt aaagtgtgtg ggcattttat gggaaggtta cattactgta
    58561 aacatgatta aattcctgtt acttggattt gactgtcagg tcacatatgt tgcaaaacat
    58621 atcaagttga ttttaagaag ctgttgcaaa agggcgttat aacgaaaggg gagtgcagaa
    58681 ggagtttgga tgggcaattg gtataaccaa tctggaataa aataattgtt taacaaataa
    58741 ttatcattat ggtgacaatt gggtgggggg aaaataaaga ctcgttagct gaatcgtgta
    58801 agaaaaaata gaattcataa attgcgtcaa taatttccaa acgctaagcc gcacaaaaga
    58861 acaaatatta ttaagcgata ctgatgttta tttagccgta ataattacgg cgagtgatta
    58921 ctacagctaa ataatattta cagattacgt cagatgcgtt tttcgtagag ccagataatc
    58981 cagcaaacca ccaagcacga cgccagaaag ggcgactaaa accccgactt ccagcagagc
    59041 aggcaggaaa gagaagtgcg tcaaatccag cgcgtccatc gtcaacagca gtaaccacac
    59101 ggccaacagg ctgacgcccg ccgccagtgt ccacatggca aaggcataac ctgccggata
    59161 ttcggtacgg gagataaagt tgtcatttac tcgtgtatat tcaagcgtct ggcggcaaac
    59221 cagcagcaaa ataagccccg gtacggcagc ggcgacggag aatagataga atgtcgacca
    59281 gccgtgtgct tcaacaaacc aacccgccac ggggccgaca taaactcgtc ctacagcgga
    59341 aagcgctgag agcagggcaa attgagtagc ggaaaatgac ttattacaca gcgtcattaa
    59401 cagcgcgaca aaggctgatg tgcccatccc gccacagagg ttttcgaaaa agacggctgc
    59461 gcccatgctg tagagatgct tatcagtaat cgacagcagc cagtaaccag cgttagacgc
    59521 accttgtaaa atgccgaaaa tcagcagcgc ccggaacagc gacaggcgct gcatcaaaat
    59581 cccaccgtac aatgcgccca caatggtcgc cagtaagcca agcgttttgt taaccacgcc
    59641 tacttcaccc gcatcaaacc cgacgccgcg aatcaaaaac gtggttgtca ggctcatggc
    59701 gaatgcgtcg cccagcttat acagcacgat aagaagcaaa ataagccagg cattattgcg
    59761 accaaagaaa tctcgcagag gtgcaacaac cgcttgttcc agcgtttttg gcacaggaat
    59821 ggtgtcggtc ggttctggtg caagcaacgt cgcgataata caagggatca acagcgcagc
    59881 catcaaccag tacatgccct gccagcccag ccatttatct gccagccaca gggctaagcc
    59941 gccggaaacc agcatcccta aacggtaacc cagcacgctg attgccgcgc ccgcaccacg
    60001 ttcttctgcc gggagcacat cggttttcca cgcatcgaag acaatatcct gggaggccga
    60061 gcaaaaagcg atcaccaccg ccagtgccgc catccagcgg agttgggtgc caggttcgag
    60121 aaaacccatc gccgcaatgg cgactaataa caggatttgc gtggcgagca gccaaccgcg
    60181 ccgccgccca aaaaatgggg gcgtgtagcg gtccatcagc ggtgaccaga ggaatttaaa
    60241 aacgtaagcc tggcctacca gagagaagaa accaatggtt ttgagatcaa tattctcgac
    60301 cgtcatccag gcctgtaagg taccggatgt cagggcgagc ggtagcccgg aagcaaaacc
    60361 caggatcagc aatatggctg aacgcggctg ttgaaaaata cgtaaatatt gactggacat
    60421 gggcgtctac aggcccggtt tgcaccgggc cagagggcag aaattaacgc gcgttctgct
    60481 tgatgaattc gtgaatgctg gtgtcctgag acatatcagc gatggtatcg gtcagcacac
    60541 tgttaaccgc atcggcgata tttttgttgg aggcctggaa cgcaccttca acgttgtagc
    60601 tggcacgata gtttttggtt attttgttgc cattctgcgc ggtagcgatg atggcgatat
    60661 ccgctttggt agcgatgttg tagcgcacgt tgccctggga cacgtcagca tacagttggc
    60721 taacgatgat ttgcagatta accgggccat tcggaccaac catgtaacca cgcgcggtca
    60781 tctgtttttc cagcacttct tgcagcagga aacgcagatc gcgggaggcg gccagggtaa
    60841 cgatttgatt atcgcgggtg acttttgcca gcgcctgatc ggtacgctga tcggcaccat
    60901 taatgcttac ggtgacgccc atcaggcttg gatcctgctg tggcagtgta atcgtcgggg
    60961 aaacttcaat agttgttggc ggttttgcgc atcctgcaag cataaacaga gcaactaacg
    61021 ggaagaggat ttttttgaac atgttcgggc tctcagagac tcttaagcgt gtttggtaaa
    61081 aattcccgcc atcataacat tgccaacggc gaggggaagt gggtaaggca tgtaaattca
    61141 tcatgttgac gaaataatca cccctggtaa aagaaacgct gatgcgaggc ctgtgtttca
    61201 atctttaaat cagtaaactt catacgcttg acggaaaaac caggacgaaa cctaaatatt
    61261 tgttgttaag ctgcaatgga aacggtaaaa gcggctagta tttaaaggga tggatgacat
    61321 ctcagcgttg tcggaggaga tatttcatga tgatacgtga gcggatagaa gaaaaattaa
    61381 gggcggcgtt ccaacccgta ttcctcgaag tagtggatga aagctatcgt cacaatgtcc
    61441 cagccggctc tgaaagccat tttaaagttg tgctggtcag cgatcgtttt acgggtgaac
    61501 gttttctgaa tcgtcatcga atgatttaca gtactttagc ggaggaactc tctactaccg
    61561 ttcatgcgct ggctctgcat acttacacta ttaaggagtg ggaagggttg caggacaccg
    61621 tctttgcctc tcctccctgt cgtggagcag gaagcatcgc gtaaaaacgc atttgcaact
    61681 gtcggcgctt tttcagtatg ttgctaaaga ttttatgaaa aacggcctgc gggccgtttt
    61741 gttttgtctg gattttgcgc tttttgccca gcattcagac gaaaattgcc cgggaattgt
    61801 gaaaaaatac gcgacagcgc gcaataaccg ttctcgactc ataaaagtga tgccgctata
    61861 atgccgcgtc ctatttgaat gctttcggga tgattctggt aacagggaat gtgattgatt
    61921 ataagaacat cccggttccg cgaagccaac aacctgtgct tgcggggtaa gagttgaccg
    61981 agcactgtga ttttttgagg taacaagatg caagtttcag ttgaaaccac tcaaggcctt
    62041 ggccgccgtg taacgattac tatcgctgct gacagcatcg agaccgctgt taaaagcgag
    62101 ctggtcaacg ttgcgaaaaa agtacgtatt gacggcttcc gcaaaggcaa agtgccaatg
    62161 aatatcgttg ctcagcgtta tggcgcgtct gtacgccagg acgttctggg tgacctgatg
    62221 agccgtaact tcattgacgc catcattaaa gaaaaaatca atccggctgg cgcaccgact
    62281 tatgttccgg gcgaatacaa gctgggtgaa gacttcactt actctgtaga gtttgaagtt
    62341 tatccggaag ttgaactgca gggtctggaa gcgatcgaag ttgaaaaacc gatcgttgaa
    62401 gtgaccgacg ctgacgttga cggcatgctg gatactctgc gtaaacagca ggcgacctgg
    62461 aaagaaaaag acagcgctgt tgaagcagaa gaccgcgtaa ccatcgactt caccggttct
    62521 gtagacagcg aagagttcga aggcggtaaa gcgtctgatt tcgtactggc gatgggccag
    62581 ggtcgtatga tcccgggctt tgaagacggt atcaaaggcc acaaagctgg cgaagagttc
    62641 accatcgacg tgaccttccc ggaagaatac cacgcagaaa acctgaaagg taaagcagcg
    62701 aaattcgcta tcaacctgaa gaaagttgaa gagcgtgaac tgccggaact gactgcagaa
    62761 ttcatcaaac gtttcggcgt tgaagatggt tccgtagaag gtctgcgcgc tgaagtgcgt
    62821 aaaaacatgg agcgcgagct gaagagcgcc atccgtaacc gcgttaagtc tcaggcgatc
    62881 gaaggtctgg taaaagctaa cgacatcgac gtaccggctg cgctgatcga cagcgaaatc
    62941 gacgttctgc gtcgccaggc tgcacagcgt ttcggtggca acgaaaaaca agctctggaa
    63001 ctgccgcgcg aactgttcga agaacaggct aaacgccgcg tagttgttgg cctgctgctg
    63061 ggcgaagtta tccgcaccaa cgagctgaaa gctgacgaag agcgcgtgaa aggcctgatc
    63121 gaagagatgg cttctgcgta cgaagatccg aaagaagtta tcgagttcta cagcaaaaac
    63181 aaagaactga tggacaacat gcgcaatgtt gctctggaag aacaggctgt tgaagctgta
    63241 ctggcgaaag cgaaagtgac tgaaaaagaa accactttca acgagctgat gaaccagcag
    63301 gcgtaattta cgcagcataa cgcgctaaat tcgcacaaag gcccgtcacc gccaggtggt
    63361 gggctttttt tgtcatgaat tttgcatgga accgtgcgaa aagcctcttt cggtgttagc
    63421 gtaacaacaa aagattgtta tgcttgaaat atggtgatgc cgtacccata acacagggac
    63481 tagctgataa tccgtccata aggttacaat cggtacagca ggttttttca attttatcca
    63541 ggagacggaa atgtcataca gcggcgaacg agataacttt gcaccccata tggcgctggt
    63601 gccgatggtc attgaacaga cctcacgcgg tgagcgctct tttgatatct attctcgtct
    63661 acttaaggaa cgcgtcattt ttctgactgg ccaggttgaa gaccacatgg ctaacctgat
    63721 tgtggcgcag atgctgttcc tggaagcgga aaacccagaa aaagatatct atctgtacat
    63781 taactcccca ggcggggtga tcactgccgg gatgtctatc tatgacacca tgcagtttat
    63841 caagcctgat gtcagcacca tctgtatggg ccaggcggcc tcgatgggcg ctttcttgct
    63901 gaccgcaggg gcaaaaggta aacgtttttg cctgccgaat tcgcgcgtga tgattcacca
    63961 accgttgggc ggctaccagg gccaggcgac cgatatcgaa attcatgccc gtgaaattct
    64021 gaaagttaaa gggcgcatga atgaacttat ggcgcttcat acgggtcaat cattagaaca
    64081 gattgaacgt gataccgagc gcgatcgctt cctttccgcc cctgaagcgg tggaatacgg
    64141 tctggtcgat tcgattctga cccatcgtaa ttgatgccag aggcgcaact gtgccgctat
    64201 acttatccag ggcggcacaa cgctgtaagc ggcttgcgcc tgagaatggc atttgcgtcg
    64261 tcgtgtgcgg cacaaagaac aaagaagagg ttttgaccca tgacagataa acgcaaagat
    64321 ggctcaggca aattgctgta ttgctctttt tgcggcaaaa gccagcatga agtgcgcaag
    64381 ctgattgccg gtccatccgt gtatatctgc gacgaatgtg ttgatttatg taacgacatc
    64441 attcgcgaag agattaaaga agttgcaccg catcgtgaac gcagtgcgct accgacgccg
    64501 catgaaattc gcaaccacct ggacgattac gttatcggcc aggaacaggc gaaaaaagtg
    64561 ctggcggtcg cggtatacaa ccattacaaa cgtctgcgca acggcgatac cagcaatggt
    64621 gtcgagttgg gcaaaagtaa cattctgctg atcggtccga ccggttccgg taaaacgctg
    64681 ctggctgaaa cgctggcgcg cctgctggat gttccgttca ccatggccga cgcgactaca
    64741 ctgaccgaag ccggttatgt gggtgaagac gttgaaaaca tcattcagaa gctgttgcag
    64801 aaatgcgact acgatgtcca gaaagcacag cgtggtattg tctacatcga tgaaatcgac
    64861 aagatttctc gtaagtcgga caacccgtct attacccgtg acgtttccgg tgaaggcgta
    64921 cagcaggcac tgttgaaact gatcgaaggt actgttgctg ccgttccacc gcagggcggg
    64981 cgtaagcatc cgcagcagga atttttgcag gttgatacct ccaagatcct gtttatttgt
    65041 ggcggtgcgt ttgccggtct ggataaagtg atttcccacc gtgtagaaac cggctccggc
    65101 attggttttg gcgcgacggt aaaagcgaag tccgacaaag caagcgaagg cgagctgctg
    65161 gcgcaggttg aaccggaaga tctgatcaag tttggtctta tccctgagtt tattggtcgt
    65221 ctgccggttg tcgcaacgtt gaatgaactg agcgaagaag ctctgattca gatcctcaaa
    65281 gagccgaaaa acgccctgac caagcagtat caggcgctgt ttaatctgga aggcgtggat
    65341 ctggaattcc gtgacgaggc gctggatgct atcgctaaga aagcgatggc gcgtaaaacc
    65401 ggtgcccgtg gcctgcgttc catcgtagaa gccgcactgc tcgataccat gtacgatctg
    65461 ccgtccatgg aagatgtcga aaaagtggtt atcgacgagt cggtaattga tggtcaaagc
    65521 aaaccgttgc tgatttatgg taagccggaa gcgcaacagg catctggtga ataattaacc
    65581 attcccatac aattagttaa ccaaaaaggg gggattttat ctccccttta atttttcctc
    65641 tattctcggc gttgaatgtg ggggaaacat ccccatatac tgacgtacat gttaatagat
    65701 ggcgtgaagc acagtcgtgt catctgatta cctggcggaa attaaactaa gagagagctc
    65761 tatgaatcct gagcgttctg aacgcattga aatccccgta ttgccgctgc gcgatgtggt
    65821 ggtttatccg cacatggtca ttcccttatt tgtcgggcgg gaaaaatcta tccgttgtct
    65881 ggaagcggcg atggaccatg ataaaaaaat tatgctggtc gcgcagaaag aagcttcaac
    65941 ggatgagccg ggtgtaaacg atcttttcac cgtcgggacc gtggcctcta tattgcagat
    66001 gctgaaactg cctgacggca ccgtcaaagt gctggtcgag gggttacagc gcgcgcgtat
    66061 ttctgcgctc tctgacaatg gcgaacactt ttctgcgaag gcggagtatc tggagtcgcc
    66121 gaccattgat gagcgggaac aggaagtgct ggtgcgtact gcaatcagcc agttcgaagg
    66181 ctacatcaag ctgaacaaaa aaatcccacc agaagtgctg acgtcgctga atagcatcga
    66241 cgatccggcg cgtctggcgg ataccattgc tgcacatatg ccactgaaac tggctgacaa
    66301 acagtccgtt ctggagatgt ccgacgttaa cgaacgtctg gaatatctga tggcaatgat
    66361 ggaatcggaa atcgatctgc tgcaggttga gaaacgcatt cgcaaccgcg ttaaaaagca
    66421 gatggagaaa tcccagcgtg agtactatct gaacgagcaa atgaaagcta ttcagaaaga
    66481 actcggtgaa atggacgacg cgccggacga aaacgaagcc ctgaagcgca aaatcgacgc
    66541 ggcgaagatg ccgaaagagg caaaagagaa agcggaagca gagttgcaga agctgaaaat
    66601 gatgtctccg atgtcggcag aagcgaccgt agtgcgtggt tatatcgact ggatggtaca
    66661 ggtaccgtgg aatgcgcgca gcaaggtcaa aaaagacctg cgtcaggcgc aggaaatcct
    66721 tgataccgac cattatggtc tggagcgcgt gaaagatcgc atccttgagt accttgcggt
    66781 tcaaagccgt gtcaacaaaa tcaagggacc gatcctctgc ctggtagggc cgccgggggt
    66841 aggtaaaacc tctcttggtc agtccattgc caaagccacc gggcgtaaat atgtccgtat
    66901 ggcgctgggc ggcgtgcgtg atgaagcgga aatccgtggt caccgccgta cttacatcgg
    66961 ttctatgccg ggtaaactga tccagaaaat ggcgaaagtg ggcgtgaaaa acccgctgtt
    67021 cctgctcgat gagatcgaca aaatgtcttc tgacatgcga ggcgatccgg cctctgcact
    67081 gcttgaagtg ctggatccag agcagaacgt agcgttcagc gaccactacc tggaagtgga
    67141 ttacgatctc agcgacgtga tgtttgtcgc gacgtcgaac tccatgaaca ttccggcacc
    67201 gctgctggat cgtatggaag tgattcgcct ctccggttat accgaagatg aaaaactgaa
    67261 catcgccaaa cgtcacctgc tgccgaagca gattgaacgt aatgcactga aaaaaggtga
    67321 gctgaccgtc gacgatagcg ccattatcgg cattattcgt tactacaccc gtgaggcggg
    67381 cgtgcgtggt ctggagcgtg aaatctccaa actgtgccgc aaagcggtta agcagttact
    67441 gctcgataag tcattaaaac atatcgaaat taacggcgat aacctgcatg actacctcgg
    67501 tgttcagcgt ttcgactatg gtcgcgctga taacgaaaac tgtgtcggtc aggtaaccgg
    67561 tctggcgtgg acggaagtgg gcggtgactt gctgaccatt gaaaccgcat gtgttccggg
    67621 taaaggcaaa ctgacctata ccggttcgct cggcgaagtg atgcaggagt ctattcaggc
    67681 ggcgttaacg gtggttcgtg cgcgtgcgga aaaactgggg atcaaccctg atttttacga
    67741 aaaacgtgac atccacgtcc acgtaccgga aggtgcgacg ccgaaagatg gtccgagtgc
    67801 cggtattgct atgtgcaccg cgctggtttc ttgcctgacc ggtaacccgg ttcgtgccga
    67861 tgtggcaatg accggtgaga tcactctgcg tggtcaggta ctgccgatcg gtggtttgaa
    67921 agaaaaactc ctggcagcgc atcgcggcgg gattaaaaca gtgctaattc cgttcgaaaa
    67981 taaacgcgat ctggaagaga ttcctgacaa cgtaattgcc gatctggaca ttcatcctgt
    68041 gaagcgcatt gaggaagttc tgactctggc gctgcaaaat gaaccgtctg gcatgcaggt
    68101 tgtgactgca aaatagtgac ctcgcgcaaa atgcactaat aaaaacaggg ctggcaggct
    68161 aattcgggct tgccagcctt tttttgtctc gctaagttag atggcggatc gggcttgccc
    68221 ttattaaggg gtgttgtaag gggatggctg gcctgatata actgctgcgc gttcgtacct
    68281 tgaaggattc aagtgcgata taaattataa agaggaagag aagagtgaat aaatctcaat
    68341 tgatcgacaa gattgctgca ggggctgata tctctaaagc tgcggctggc cgtgcgttag
    68401 atgctattat tgcttccgta actgaatctc tgaaagaagg ggatgatgta gcactggtag
    68461 gttttggtac ttttgccgtt aaagagcgtg ctgcccgtac tggccgcaac ccgcagaccg
    68521 gtaaagagat caccatcgct gctgctaaag taccgagctt ccgtgcaggt aaagcactga
    68581 aagacgcggt aaactaagcg ttgtccccgg tggggatgtg acgaagttca agggcgcatc
    68641 tactgatgtg ccttttttat ttgtattcgg tgactttctg cgtcttgtgg gctgacaatt
    68701 gcccccgttt cttgtcacaa taggcctttg cgcgcatcga tacgttgcgt gaggtacaca
    68761 gtcatctaca gcggagtgtt gttacaccat gatggacagc ttacgcacgg ctgcaaacag
    68821 tctcgtgctc aagattattt tcggtatcat tatcgtgtcg ttcatattga ccggcgtgag
    68881 tggttacctg attggcggag gcaataacta cgccgcaaaa gtgaatgacc aggaaatcag
    68941 ccgtgggcag ttcgagaacg cctttaacag cgagcgtaat cgcatgcagc aacagctggg
    69001 cgatcaatac tccgagctgg cagcgaatga aggctatatg aaaaccctgc gtcaacaggt
    69061 gctgaatcgt ctgatcgacg aggcgctgtt ggatcagtac gctcgtgagc tgaaactggg
    69121 tatcagtgat gagcaggtta aacaggcgat tttcgcgacc ccagccttcc aggttgacgg
    69181 caaatttgat aacagccgct ataacggtat cctcaaccag atggggatga ccgccgatca
    69241 gtacgcccag gcgctgcgta accagctcac tacccaacag ctgattaacg gtgttgccgg
    69301 taccgatttt atgctgaaag gtgaaaccga cgagctggcg gcactggtcg cgcaacaacg
    69361 cgtggtgcgt gaggcgacta tcgatgttaa cgcgctggcg gcgaagcagc ctgtgaccga
    69421 acaggagatt gccagctact acgaacaaaa caaaaacaat ttcatgacgc cggaacaatt
    69481 ccgcgtgagt tacatcaagc tggatgccgc aacgatgcag caaccggtta gcgatgcgga
    69541 tatccagagc tactacgatc agcatcagga tcaattcacc cagccgcagc gtacccgcta
    69601 cagcatcatc cagaccaaaa ctgaagatga agcgaaagcg gtacttgatg agctgaataa
    69661 aggcggtgat tttgctgcat tagccaaaga aaaatctgcc gatattatct ctgcccgtaa
    69721 cggcggcgat atgggttggt tagaagatgc cactatcccc gacgaactga aaaatgctgg
    69781 tctgaaagaa aaaggccaac tgtctggtgt catcaaatct tcggtcggtt tcctgattgt
    69841 acgtctggac gacattcagc cagcgaaagt gaaatcgtta gacgaagtac gtgacgacat
    69901 tgcggcgaaa gtgaaacacg aaaaagccct cgatgcgtac tatgcgctgc agcagaaagt
    69961 gagcgatgcg gcaagcaacg acaccgagtc tctggccggt gcagagcaag ctgccggcgt
    70021 taaagccact cagacgggtt ggttcagcaa agataacctg ccggaagagt tgaacttcaa
    70081 gccggttgcc gacgctatct ttaacggcgg tctggtaggt gaaaacggcg cgccgggcat
    70141 caactctgac atcatcaccg tagacggcga ccgtgcattc gtgctgcgca tcagcgagca
    70201 caaaccggaa gcggtgaaac cgttggcaga tgttcaggaa caagttaagg cactggttca
    70261 gcacaacaaa gctgaacaac aggcgaaagt ggatgctgag aaactgctgg ttgatttgaa
    70321 agccggcaaa ggtgcggaag ctatgcaggc tgccggtctg aaatttggcg agccgaaaac
    70381 cttaagccgt tccggtcgtg acccgattag ccaggcggcg tttgcactgc cactgccagc
    70441 gaaagacaaa ccgagctacg gtatggcgac cgatatgcaa ggtaatgtgg ttctgctggc
    70501 gctggatgaa gtgaaacaag gttcaatgcc ggaagatcag aaaaaagcga tggtgcaggg
    70561 tatcacccag aacaacgcac aaatcgtctt tgaagctctg atgagtaacc tgcgtaaaga
    70621 ggcgaaaatc aaaattggcg atgcgctgga acagcaataa tcctgaagcc gcctcgcaaa
    70681 aaaatgcgtt gcagctgtaa caagaaaagg tcgctttcgc ggccttttcc atttctgaac
    70741 attgccattt gtttactgtt ttcactgccg ttaaggtgat tccactgtta acaaacaagg
    70801 agaaaacagt atgaaacacg gaattaaagc actgctcatt accctgtccc tggcctgtgc
    70861 cggaatgtct catagcgcgc tggcggcagc ttctgtggcg aaaccgacgg cggtagaaac
    70921 caaagcggaa gctcctgcag cacaaagtaa agcagcagta ccggcgaaag ccagtgacga
    70981 agaaggcacc cgggtcagca ttaataatgc cagcgcggaa gagctagccc gcgcgatgaa
    71041 tggcgttggc ctgaagaaag cgcaggcgat tgtcagttat cgcgaagagt acggtccgtt
    71101 taaaacagtg gaggatctaa agcaggtgcc ggggatgggc aattcgctgg tggaacgtaa
    71161 tctggcggta ttaaccctgt aattaatttg catagtggca attttttgcc agactgaaga
    71221 ggtcatacca gttatgacct ctgtacttat aacaacaacg taaggttatt gcgctatgca
    71281 aacacaaata aaagttcgtg gatatcatct cgacgtttac cagcacgtca acaacgcccg
    71341 ctaccttgaa tttctcgaag aagcccgctg ggatgggttg gaaaatagcg acagttttca
    71401 gtggatgacg gcccataaca tcgccttcgt cgtggtcaat atcaatatta actatcgtcg
    71461 cccagcggta ttaagtgacc tgttaactat taccagtcag ttgcagcaat taaacggtaa
    71521 aagcggcatc ttaagccagg tcattacact ggagccggaa gggcaggtgg tagcggatgc
    71581 gcttattacg tttgtttgta ttgatcttaa aacgcagaaa gcattagctc tggaagggga
    71641 attgcgcgaa aagctggagc agatggttaa gtaaacgttt tgtggtgccg gatgctcaag
    71701 ccgcatccgg cgacacccgg aataattacc tcaacccggt tttctgcttc atcgctgcca
    71761 tcaccgtcgg tttatcggcc agataatgat tcaaaccgtt ggcgcgtaaa ttacatgccg
    71821 cacaatgacc gcaaccgtcg cctttaatgc cgttatagca ggtcaacgtt tcgttacgga
    71881 ctaaatccag tttgccgtaa taatctgcca gcgcccaggt ttccgcttta tcaatccaca
    71941 tcagcggcgt ttcaaaacga atatctttcg ccatgcccaa actgacggca tggtttagtg
    72001 ctttcacaaa ctcatcgcgg caatccgggt agccggagaa atccgtttca cagacgccgg
    72061 taattacggc ttctgctttt acctgatacg catatattgc cgccagcgtc aggaacaaaa
    72121 tattacgccc tgggacaaac gtattcggga tgccatcggc ttcaggttca taatcaggca
    72181 ccggaatgct gtcacgtgtc aggctgctga ccgccagctc gttgagcagc gttacatcaa
    72241 gaaccttatg cgcgcgtgct cccagtttca gcgccagttc gcgtgccacg tcgatttctg
    72301 cgcgatgtcg ctgaccgtaa tcgaacgtca cgcaatggac ttcatcatat tgttgtaatg
    72361 cctgcaccag acaggtggtg gaatcctggc ctccactgaa cacaacgaca gcacgtttca
    72421 tagataatcc tgcttgaaca ataaaagcgt tatggtaacg cctgcgatta acccggacca
    72481 gctattcatt gcgacggcgc gggaagccag gcttcggtaa attcaaacca gccgcgcggt
    72541 gtcagtcgca caccgttcac gccgggaggg gcactaatgc gatagtgata gttgaacagc
    72601 ggcgtcagcg tcgcgtctgc cattaactgg ctaaaaaccg ctttcagggc attaaatcgg
    72661 ttttcctcat caggcattac ttgcaccgcg tccagcgtcg attgcagatg tgcatatgct
    72721 ggagcgtcga aaacatgtgg ccatagtgga tcgcaacgta gccattgctc cagagtatat
    72781 tccggtgctt cgccaattaa tctgtcgccc atcatgaggt ctgcgtgtgc cagtagggtc
    72841 gtgtcgtccc agttttttgc gttatgaaaa ataattgtga gttcacagcc ttccgctgcc
    72901 agtgtcgcct gtaggcgttc tgccatggta tgaagttcta tcggtaggtg ataaaccagc
    72961 gtcaaggttt tcggtagttt gacttcatcc ggtacttgcc agtgcggaat agtccagcct
    73021 ggcagtaatg catgactggc ggtgatcagg ttttctccga cttctaacgt ttgtaataaa
    73081 ccggattgat gaataatgga gatcactttt cgcgcctgcc agagggagag tcggggactt
    73141 ttgcgcaacg ttaaatagca aaaacctaaa ctgatgccac tactgacctg gctgacccgt
    73201 tgcagctcct ccggtttgcc gatggtgatt tgcacgggat gccgacaact ggttcccaaa
    73261 tctttttcga aaagcggcgg agttatccag tactcaaccg ctttaagcag cggatgacgc
    73321 aaatggtaat aatcatggct ctccaggcgc accagctccg ctgtgaattg tgttaagcga
    73381 aaaggaccag tgccgatcag tgggaattgc ggatgcgcca gatggctgca atagctcgcc
    73441 agccggtgcg caagccagta atcggggcga tgtaaaaaga aggtcagaca ctgcggatgg
    73501 gtgacttcaa tacgcttcac gctaataaat aattgatcca gtgctggcag ttgtaacagc
    73561 atcaataatc gctggtgtaa gtgtgaggct tttactgcat cgccgttatg ccagtgtagg
    73621 gttgaacgaa gataaaagtc ccagcgtaac ccgtcagtag aggtttccca gtgatgcgct
    73681 aaatcgccaa tcgggcgctg agtattatta tcgaagcggg tcaggccgga aaatatctgc
    73741 ccggcgagat gctgctcggc acggccgggc aaaaagcctg gttgtagcgg ttcgagcggg
    73801 cgatagtagg gaatacgcaa cgtgggtgta tcgttttgcc attgtccgcc cataaacggc
    73861 tgtaacagag tgcgtagctc acctggggcc agttgcgcca gctccagcac atcttgctgc
    73921 tttccggttt ccagtgcctg ttccatcatc gcattgcgta gcgattccgg cgtgaccaga
    73981 aagcgtaatt gtccgcgctt tccgcgtcct gactgcgcct gccactccag ccatcccgcc
    74041 tcctgtgcct gacgcaacag cgtacgaaca tggcgttcgc tgcaaaaaca gcgttcggcc
    74101 agttcgctga cggtgacggt ttgcggcttt ccggctgaag gttgccacag acgttgatac
    74161 tggttaagac ggttgagcaa tcgcatataa acccggaaca atattattta actattcact
    74221 attacttccg tatatatcag gtgatactca atctccatta accgtgtcac agagtggaga
    74281 aagaaatggc tcgtctggca gcatttgata tggatggcac tttattgatg cctgaccatc
    74341 atttaggtga gaaaaccctc tctactttgg cgcgactgcg tgaacgcgac attaccctca
    74401 cttttgccac ggggcgtcat gcgctggaga tgcagcatat tctcggggcg ctatcgctgg
    74461 atgcatattt gattaccggc aacggaacgc gcgtgcattc tctggaaggt gaacttttac
    74521 atcgtgatga tttacctgcg gatgtcgcgg agctggtgct gtatcagcaa tgggataccc
    74581 gagccagcat gcatatcttc aatgacgacg gttggtttac cgggaaagag atccctgcgt
    74641 tgttgcaggc atttgtctat agcggttttc gttatcagat aatcgatgtc aaaaaaatgc
    74701 cactcggcag cgtcaccaag atctgcttct gtggcgatca cgacgatctt acacgcttgc
    74761 agatccagct atacgaagca ttaggcgagc gtgcacattt gtgtttttcc gccacggatt
    74821 gcctcgaagt gctgccggtg ggctgcaata aaggcgctgc attgacggtg ctgacccaac
    74881 atctaggttt atcgttgcgc gattgcatgg cctttggtga tgcgatgaac gatcgcgaaa
    74941 tgttaggcag cgtcggtagc gggtttatta tgggcaatgc gatgccgcaa ctgcgcgcgg
    75001 agctcccgca tttaccggtg attggacatt gccgaaatca ggctgtctct cactatttga
    75061 cgcactgact ggactatcca catctacctt attcccccga ataatgagat cccttccagc
    75121 accgggcaat tgcccggttt tttttgcgtt gaatttgtca ttttgtgccg tggtgtttaa
    75181 accgcacaga ataaattgtc gtgatttcac ctttaaaata gaattaaaag agaaaaaaat
    75241 tctctgtgga agggctatgt tagataaaat tgaccgtaag ctgctggcct tactgcagca
    75301 ggattgcacc ctctctttgc aggcactggc tgaagccgtt aatctgacaa ccaccccttg
    75361 ctggaagcgc ctgaaacggc tggaggacga cggtatcctt atcggcaaag tcgccctgct
    75421 ggatccggaa aaaataggcc tcggcctgac cgcctttgtg ctgataaaaa cgcaacatca
    75481 cagtagcgaa tggtattgcc gctttgtcac ggtggttacc gaaatgccag aagtgctggg
    75541 gttctggcgc atggctggtg aatacgatta tctgatgcgc gtccaggttg ccgacatgaa
    75601 acgctacgac gagttttata agcgtctggt aaacagcgtg ccggggctgt cggacgtcac
    75661 ttccagcttc gcgatggaac agattaaata caccacttct ttacccatcg aataaatatc
    75721 cagaatcagg tcaggacaca acgcgtgcga ttatttgctc aattaagctg gtatttccgt
    75781 cgggaatggc gtcgctatct cggggctgtc gccttgcttg tcattatcgc gatgctgcaa
    75841 ctggttccgc caaaagtggt tggtattgtt gtcgatggcg tgacagaaca acactttact
    75901 accgggcaga tcctgatgtg gatcgccacc atggtgctga ttgccgttgt ggtttatttc
    75961 ctgcgttacg tctggcgggt attgctgttt ggtgcgtctt atcaactggc tgttgaactg
    76021 cgtgaagatt attaccgtca gctaagccgg cagcatcctg agttttacct gcgtcatcgc
    76081 acgggtgacc tcatggctcg tgcgaccaat gacgtcgatc gcgtcgtgtt tgcagccgga
    76141 gaaggggtgc tgacgctggt ggattcactg gtgatgggct gcgctgtgtt gattatgatg
    76201 tctacgcaaa ttagctggca gttgacctta tttgccctgt tgccgatgcc agtgatggcg
    76261 atcatgatca agcgcaacgg cgatgccttg catgaacgct ttaagctggc acaggcggcg
    76321 ttttctagtc ttaatgaccg cacccaggaa agcctcacca gtatccgcat gatcaaagcc
    76381 tttggtctgg aagatcgcca gtcggcgtta tttgccgcgg atgccgaaga taccggcaaa
    76441 aaaaacatgc gggtggcgcg tattgatgct cgtttcgacc cgaccatcta tatcgcgatt
    76501 ggtatggcga acttgctggc gattggcggc ggtagttgga tggtggtgca gggcagttta
    76561 acgctgggcc agctcaccag ttttatgatg tatttaggtc tgatgatttg gccaatgctg
    76621 gcgctggcat ggatgtttaa cattgtggaa cgtggtagtg ctgcatacag ccgtattcgc
    76681 gcgatgctgg cggaagcgcc ggtggtgaac gatggtagcg aacccgtgcc ggaagggcgt
    76741 ggcgaactgg atgtaaatat tcaccagttc acgtatccgc agactgacca tcctgcgctg
    76801 gaaaacgtca atttcgccct gaaacccggt cagatgctgg gtatctgcgg gccgactggt
    76861 tccggcaaaa gtaccctgtt gtcgctcatt cagcgtcatt tcgacgtcag cgagggggat
    76921 attcgctttc atgatattcc tctgacgaag ttacaactcg atagctggcg tagccgcctg
    76981 gcggtagtta gccagacacc gttccttttt tccgacaccg tagcgaacaa catcgcgttg
    77041 ggttgcccga atgccaccca gcaagagatt gagcatgtcg cgcggttagc cagcgtacat
    77101 gacgatattt tgcgtctacc gcaaggttac gatacagaag tgggcgagcg cggcgtgatg
    77161 ctttccggtg ggcaaaaaca gcgtatctcc attgctcgag cgttattagt caacgcggaa
    77221 atcctcatcc ttgatgatgc gctttcggcg gtggacggac gcacagagca ccagatcctg
    77281 cataacctgc gtcagtgggg acagggaaga acggtaatca tcagtgccca tcgcctttcg
    77341 gcactgacgg aagccagt
//
LOCUS       DAJVUJ010000004        69384 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.004, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000004 DAJVUJ010000000
VERSION     DAJVUJ010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 69384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69384
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.004"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..772
                     /locus_tag="OQQ48_000260"
     CDS             <1..772
                     /locus_tag="OQQ48_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1951151.1"
                     /translation="TTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGD
                     TSRYGPVNIGANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVK
                     LSIQGNLTAPQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTS
                     KIKNSLQMRIDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPV
                     DPSGHGTVNMRAWPVNLVGGELETGKFQGTATITVMVR"
     gene            complement(815..1675)
                     /gene="rsmI"
                     /locus_tag="OQQ48_000261"
     CDS             complement(815..1675)
                     /gene="rsmI"
                     /locus_tag="OQQ48_000261"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCS1951152.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALVAEIHGVKKNA
                     LYKYALEQQG"
     gene            1739..3766
                     /gene="lpoA"
                     /locus_tag="OQQ48_000262"
     CDS             1739..3766
                     /gene="lpoA"
                     /locus_tag="OQQ48_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HCS1951153.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKKQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAADVAEQPQPQTADGVASPAQASVSDLTGDQPAAQPVP
                     VSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNVEEL
                     LKSNTPLNVLVLNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIPRSS
                     LGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATTDSG
                     MTTNNPTLQTMPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGAMLYAS
                     SRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMGVDA
                     WSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            3724..4119
                     /locus_tag="OQQ48_000263"
     CDS             3724..4119
                     /locus_tag="OQQ48_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HCS1951154.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEVQARRWLEGKGLRFVAANVN
                     ERGGEIDLIMREGWTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            4139..4729
                     /gene="diaA"
                     /locus_tag="OQQ48_000264"
     CDS             4139..4729
                     /gene="diaA"
                     /locus_tag="OQQ48_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HCS1951155.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            4739..5314
                     /gene="yraP"
                     /locus_tag="OQQ48_000265"
     CDS             4739..5314
                     /gene="yraP"
                     /locus_tag="OQQ48_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HCS1951156.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(5428..6468)
                     /locus_tag="OQQ48_000266"
     CDS             complement(5428..6468)
                     /locus_tag="OQQ48_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCS1951157.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQHYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAICLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEARSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(6541..7176)
                     /locus_tag="OQQ48_000267"
     CDS             complement(6541..7176)
                     /locus_tag="OQQ48_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HCS1951158.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            7304..7822
                     /gene="yhbO"
                     /locus_tag="OQQ48_000268"
     CDS             7304..7822
                     /gene="yhbO"
                     /locus_tag="OQQ48_000268"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HCS1951159.1"
                     /translation="MSKKIAVLISDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(7802..8245)
                     /locus_tag="OQQ48_000269"
     CDS             complement(7802..8245)
                     /locus_tag="OQQ48_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HCS1951160.1"
                     /translation="METLITISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     HRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            8296..8598
                     /gene="yhbQ"
                     /locus_tag="OQQ48_000270"
     CDS             8296..8598
                     /gene="yhbQ"
                     /locus_tag="OQQ48_000270"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HCS1951161.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(8585..9088)
                     /locus_tag="OQQ48_000271"
     CDS             complement(8585..9088)
                     /locus_tag="OQQ48_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCS1951162.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(9082..9606)
                     /locus_tag="OQQ48_000272"
     CDS             complement(9082..9606)
                     /locus_tag="OQQ48_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HCS1951163.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADVSDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLHVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            9815..10795
                     /locus_tag="OQQ48_000273"
     CDS             9815..10795
                     /locus_tag="OQQ48_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCS1951164.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPNIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            10804..11682
                     /locus_tag="OQQ48_000274"
     CDS             10804..11682
                     /locus_tag="OQQ48_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCS1951165.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELTSMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLLLTANS
                     DCNGYWRRLAGLELQA"
     gene            11888..12895
                     /locus_tag="OQQ48_000275"
     CDS             11888..12895
                     /locus_tag="OQQ48_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HCS1951166.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(13013..14257)
                     /gene="mtr"
                     /locus_tag="OQQ48_000276"
     CDS             complement(13013..14257)
                     /gene="mtr"
                     /locus_tag="OQQ48_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HCS1951167.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAMGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(14411..16300)
                     /gene="deaD"
                     /locus_tag="OQQ48_000277"
     CDS             complement(14411..16300)
                     /gene="deaD"
                     /locus_tag="OQQ48_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HCS1951168.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(16293..16373)
                     /gene="yrbN"
                     /locus_tag="OQQ48_000278"
     CDS             complement(16293..16373)
                     /gene="yrbN"
                     /locus_tag="OQQ48_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HCS1951169.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(16480..17364)
                     /gene="nlpI"
                     /locus_tag="OQQ48_000279"
     CDS             complement(16480..17364)
                     /gene="nlpI"
                     /locus_tag="OQQ48_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HCS1951170.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDSDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(17473..19608)
                     /gene="pnp"
                     /locus_tag="OQQ48_000280"
     CDS             complement(17473..19608)
                     /gene="pnp"
                     /locus_tag="OQQ48_000280"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCS1951171.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRSVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(19856..20125)
                     /gene="rpsO"
                     /locus_tag="OQQ48_000281"
     CDS             complement(19856..20125)
                     /gene="rpsO"
                     /locus_tag="OQQ48_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCS1951172.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(20274..21218)
                     /gene="truB"
                     /locus_tag="OQQ48_000282"
     CDS             complement(20274..21218)
                     /gene="truB"
                     /locus_tag="OQQ48_000282"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCS1951173.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICFGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITIYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(21218..21619)
                     /gene="rbfA"
                     /locus_tag="OQQ48_000283"
     CDS             complement(21218..21619)
                     /gene="rbfA"
                     /locus_tag="OQQ48_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCS1951174.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(21783..24431)
                     /gene="infB"
                     /locus_tag="OQQ48_000284"
     CDS             complement(21783..24431)
                     /gene="infB"
                     /locus_tag="OQQ48_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCS1951175.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSNQQDDMTKN
                     AQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDNAEPTEDSS
                     DYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADREEARAAVRG
                     GKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIKAMMKLGAM
                     ATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAPVVTIMGHV
                     DHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAAFTSMRARG
                     AQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPDRVKNELSQ
                     YGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMASGAVIESFL
                     DKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSIPVEILGLS
                     GVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMTEGEVHEVN
                     IVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASNAILVGFNV
                     RADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLAEVRDVFKS
                     PKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVRNGMECGIG
                     VKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(24456..25943)
                     /gene="nusA"
                     /locus_tag="OQQ48_000285"
     CDS             complement(24456..25943)
                     /gene="nusA"
                     /locus_tag="OQQ48_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCS1951176.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLELGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGIDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGVTDEKAGALIMAARNICWFGDEA"
     gene            complement(25971..26423)
                     /gene="rimP"
                     /locus_tag="OQQ48_000286"
     CDS             complement(25971..26423)
                     /gene="rimP"
                     /locus_tag="OQQ48_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCS1951177.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGHTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(26630..26706)
                     /locus_tag="OQQ48_000287"
     tRNA            complement(26630..26706)
                     /locus_tag="OQQ48_000287"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(26670..26672),aa:Met,seq:cat)
     gene            27054..28397
                     /gene="argG"
                     /locus_tag="OQQ48_000288"
     CDS             27054..28397
                     /gene="argG"
                     /locus_tag="OQQ48_000288"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HCS1951178.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSNDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENMENKGQ"
     gene            complement(28405..30030)
                     /locus_tag="OQQ48_000289"
     CDS             complement(28405..30030)
                     /locus_tag="OQQ48_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCS1951179.1"
                     /translation="MTVFNKFARSFKSHWLLYLCVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVVKMLGMYIPYLCAFAFLSLLFWAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKKTFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISSAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPQTLEEVIAHYKGDS
                     RVVDANHDVFDYVMFRKEFTEDKQGNPPPEGQG"
     gene            complement(30489..30575)
                     /locus_tag="OQQ48_000290"
     tRNA            complement(30489..30575)
                     /locus_tag="OQQ48_000290"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30539..30541),aa:Leu,seq:gag)
     gene            complement(30590..30922)
                     /gene="secG"
                     /locus_tag="OQQ48_000291"
     CDS             complement(30590..30922)
                     /gene="secG"
                     /locus_tag="OQQ48_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCS1951180.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(31150..32487)
                     /gene="glmM"
                     /locus_tag="OQQ48_000292"
     CDS             complement(31150..32487)
                     /gene="glmM"
                     /locus_tag="OQQ48_000292"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCS1951181.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIISKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(32480..33328)
                     /gene="folP"
                     /locus_tag="OQQ48_000293"
     CDS             complement(32480..33328)
                     /gene="folP"
                     /locus_tag="OQQ48_000293"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HCS1951182.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWLSVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(33418..35352)
                     /gene="ftsH"
                     /locus_tag="OQQ48_000294"
     CDS             complement(33418..35352)
                     /gene="ftsH"
                     /locus_tag="OQQ48_000294"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCS1951183.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMIEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(35452..36081)
                     /gene="rlmE"
                     /locus_tag="OQQ48_000295"
     CDS             complement(35452..36081)
                     /gene="rlmE"
                     /locus_tag="OQQ48_000295"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HCS1951184.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            36207..36500
                     /gene="yhbY"
                     /locus_tag="OQQ48_000296"
     CDS             36207..36500
                     /gene="yhbY"
                     /locus_tag="OQQ48_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCS1951185.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(36657..37133)
                     /gene="greA"
                     /locus_tag="OQQ48_000297"
     CDS             complement(36657..37133)
                     /gene="greA"
                     /locus_tag="OQQ48_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCS1951186.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            37381..38814
                     /gene="dacB"
                     /locus_tag="OQQ48_000298"
     CDS             37381..38814
                     /gene="dacB"
                     /locus_tag="OQQ48_000298"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCS1951187.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMIATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVIPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(38854..40026)
                     /gene="cgtA"
                     /locus_tag="OQQ48_000299"
     CDS             complement(38854..40026)
                     /gene="cgtA"
                     /locus_tag="OQQ48_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HCS1951188.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(40042..41007)
                     /locus_tag="OQQ48_000300"
     CDS             complement(40042..41007)
                     /locus_tag="OQQ48_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCS1951189.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(41134..41391)
                     /gene="rpmA"
                     /locus_tag="OQQ48_000301"
     CDS             complement(41134..41391)
                     /gene="rpmA"
                     /locus_tag="OQQ48_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCS1951190.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(41412..41723)
                     /gene="rplU"
                     /locus_tag="OQQ48_000302"
     CDS             complement(41412..41723)
                     /gene="rplU"
                     /locus_tag="OQQ48_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCS1951191.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            41982..42953
                     /gene="ispB"
                     /locus_tag="OQQ48_000303"
     CDS             41982..42953
                     /gene="ispB"
                     /locus_tag="OQQ48_000303"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HCS1951192.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            43182..43460
                     /gene="sfsB"
                     /locus_tag="OQQ48_000304"
     CDS             43182..43460
                     /gene="sfsB"
                     /locus_tag="OQQ48_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HCS1951193.1"
                     /translation="MESNFIDWHPPDIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(43508..44767)
                     /gene="murA"
                     /locus_tag="OQQ48_000305"
     CDS             complement(43508..44767)
                     /gene="murA"
                     /locus_tag="OQQ48_000305"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCS1951194.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIKRVKGE"
     gene            complement(44822..45076)
                     /gene="ibaG"
                     /locus_tag="OQQ48_000306"
     CDS             complement(44822..45076)
                     /gene="ibaG"
                     /locus_tag="OQQ48_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HCS1951195.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(45236..45529)
                     /gene="mlaB"
                     /locus_tag="OQQ48_000307"
     CDS             complement(45236..45529)
                     /gene="mlaB"
                     /locus_tag="OQQ48_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HCS1951196.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLQLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(45529..46164)
                     /gene="mlaC"
                     /locus_tag="OQQ48_000308"
     CDS             complement(45529..46164)
                     /gene="mlaC"
                     /locus_tag="OQQ48_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HCS1951197.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(46183..46734)
                     /gene="mlaD"
                     /locus_tag="OQQ48_000309"
     CDS             complement(46183..46734)
                     /gene="mlaD"
                     /locus_tag="OQQ48_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HCS1951198.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(46739..47521)
                     /gene="mlaE"
                     /locus_tag="OQQ48_000310"
     CDS             complement(46739..47521)
                     /gene="mlaE"
                     /locus_tag="OQQ48_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HCS1951199.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(47529..48338)
                     /gene="mlaF"
                     /locus_tag="OQQ48_000311"
     CDS             complement(47529..48338)
                     /gene="mlaF"
                     /locus_tag="OQQ48_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HCS1951200.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            48548..49525
                     /locus_tag="OQQ48_000312"
     CDS             48548..49525
                     /locus_tag="OQQ48_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HCS1951201.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            49539..50525
                     /gene="kdsD"
                     /locus_tag="OQQ48_000313"
     CDS             49539..50525
                     /gene="kdsD"
                     /locus_tag="OQQ48_000313"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HCS1951202.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            50546..51112
                     /gene="kdsC"
                     /locus_tag="OQQ48_000314"
     CDS             50546..51112
                     /gene="kdsC"
                     /locus_tag="OQQ48_000314"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HCS1951203.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSELLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            51109..51684
                     /gene="lptC"
                     /locus_tag="OQQ48_000315"
     CDS             51109..51684
                     /gene="lptC"
                     /locus_tag="OQQ48_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HCS1951204.1"
                     /translation="MSKARRWVTIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            51653..52210
                     /gene="lptA"
                     /locus_tag="OQQ48_000316"
     CDS             51653..52210
                     /gene="lptA"
                     /locus_tag="OQQ48_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HCS1951205.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            52217..52942
                     /gene="lptB"
                     /locus_tag="OQQ48_000317"
     CDS             52217..52942
                     /gene="lptB"
                     /locus_tag="OQQ48_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HCS1951206.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            52990..54423
                     /gene="rpoN"
                     /locus_tag="OQQ48_000318"
     CDS             52990..54423
                     /gene="rpoN"
                     /locus_tag="OQQ48_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HCS1951207.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTSETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            54446..54733
                     /gene="hpf"
                     /locus_tag="OQQ48_000319"
     CDS             54446..54733
                     /gene="hpf"
                     /locus_tag="OQQ48_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HCS1951208.1"
                     /translation="MQLNITGNNVEITEALREFVRAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            54851..55342
                     /gene="ptsN"
                     /locus_tag="OQQ48_000320"
     CDS             54851..55342
                     /gene="ptsN"
                     /locus_tag="OQQ48_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HCS1951209.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            55388..56242
                     /gene="rapZ"
                     /locus_tag="OQQ48_000321"
     CDS             55388..56242
                     /gene="rapZ"
                     /locus_tag="OQQ48_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCS1951210.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            56239..56511
                     /gene="npr"
                     /locus_tag="OQQ48_000322"
     CDS             56239..56511
                     /gene="npr"
                     /locus_tag="OQQ48_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HCS1951211.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            56725..57357
                     /gene="yrbL"
                     /locus_tag="OQQ48_000323"
     CDS             56725..57357
                     /gene="yrbL"
                     /locus_tag="OQQ48_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HCS1951212.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAYLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHHISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(57354..58082)
                     /gene="mtgA"
                     /locus_tag="OQQ48_000324"
     CDS             complement(57354..58082)
                     /gene="mtgA"
                     /locus_tag="OQQ48_000324"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HCS1951213.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHRGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(58079..58732)
                     /gene="elbB"
                     /locus_tag="OQQ48_000325"
     CDS             complement(58079..58732)
                     /gene="elbB"
                     /locus_tag="OQQ48_000325"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HCS1951214.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFATLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(58962..61298)
                     /gene="arcB"
                     /locus_tag="OQQ48_000326"
     CDS             complement(58962..61298)
                     /gene="arcB"
                     /locus_tag="OQQ48_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HCS1951215.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(61394..62323)
                     /locus_tag="OQQ48_000327"
     CDS             complement(61394..62323)
                     /locus_tag="OQQ48_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCS1951216.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            62998..67458
                     /gene="gltB"
                     /locus_tag="OQQ48_000328"
     CDS             62998..67458
                     /gene="gltB"
                     /locus_tag="OQQ48_000328"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HCS1951217.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLNVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            67471..68889
                     /gene="gltD"
                     /locus_tag="OQQ48_000329"
     CDS             67471..68889
                     /gene="gltD"
                     /locus_tag="OQQ48_000329"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HCS1951218.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEQFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENKDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            69073..>69384
                     /locus_tag="OQQ48_000330"
     CDS             69073..>69384
                     /locus_tag="OQQ48_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1951219.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSAKNLRQMRLFYLFFQHVEI
                     RQ"
BASE COUNT        16948 a        18848 c        17249 g        16339 t
ORIGIN      
        1 aaccacatta caggcaaacg acattccagg actcacaacc gaccaggttg tctctgttaa
       61 cacccgattc acacagataa aaagcagcac cgtatattct gctgcaaccc aaacgggtgt
      121 ttgccagggt gacacgtctc gttatggacc cgttaatatt ggtgcaaata ccacctttac
      181 cctgtatgtc accaagccat ttctcggctc gatgaccatt ccgaaaacgg atattgccgt
      241 cattaaaggc gcgtgggtcg atggaatggg aagcccgtct acaggtgact tccatgattt
      301 agtcaagtta tcgattcagg gaaatctcac cgccccacag tcgtgcaaaa ttaatcaggg
      361 cgatgttatt aaggttaatt ttggattcat caatggtcag aagtttacca cccgcaatgc
      421 catgccagac ggttttactc cagtagactt tgatatcact tatgactgtg gtgatacttc
      481 aaagattaaa aactcgttgc aaatgcgcat cgacggtaca actggggtag tagaccagta
      541 caacctggtc gccaggcgaa gaagttcaga caatgtgccc gatgtcggta ttcgtattga
      601 aaatctcggc ggcggagttg caaatattcc ttttcagaac ggtatccttc ccgttgatcc
      661 ttccgggcat ggcaccgtca atatgcgcgc ctggccagtt aatctggtcg gtggtgagct
      721 ggaaacagga aaatttcagg gcacagccac cattaccgtc atggtgcggt aaaagaagac
      781 gttgcgtttt gattaacgca acgcttcggc actgttaccc ctgctgctcc agcgcatact
      841 tatacagcgc atttttcttc acgccatgaa tttctgcgac cagcgccgcc gcttttttca
      901 gcggcagttc tgcctgtagc agcgccagcg tgcgcagggc atcggcgggt aagtcttctt
      961 cctgtgcttt atgaccttcg acaatcagca ccatttcgcc tttgcgacgg ttttcatctt
     1021 cctttaccca cgccagcagt tcgccaacgg gcgcgccgtg aatggtttcc caggttttgg
     1081 tcagctcacg cgccagaacc acgtagcggg attcgcctaa taccgcaacg atatcttcca
     1141 ggctatctaa cagacggtgg gtggattcat aaaaaatcag cgtgcgcggc tccgcttcaa
     1201 tggcttttag cgcatcacgg cggccttttg atttggcagg taaaaagcct tcgtaacaga
     1261 aacggtcaga gggtaaaccc gcggcgctta acgcagtgat agcagcacac ggcccgggta
     1321 gcggcaccac gcgaatcccc gcttcacggc aggtacgcac cagatggtag ccaggatcgt
     1381 taattagcgg cgttccggca tcggaaacca gcgcaatgtt ttgcccctct tgcagcttcg
     1441 ccagcagcgt ttcggctttt tgttgttcgt tatggtcgtg cagcgcaaac aaccgggcat
     1501 taatcccaaa atgttgcagc aataaaccgg tgtgacgagt atcctcggcg gcaatcagat
     1561 caacggcctg taatacctct aacgcacgct gggtgatatc cgccagattg ccgattggcg
     1621 tcggtacaat gtaaagctgg ccctgagaat tatccgccga ttggtgttgt ttcattgtgt
     1681 cgtccgtatt gccgatttaa tattgagcat tgcgtaaaaa aatatcactg gatacattat
     1741 ggtaccctca acattttctc gtttgaaagc cgcgcgttgt ctgcctgttg ttctggcagc
     1801 cctgattttc gccggttgtg gcacccatac tcccgatcag tccactgctt atatgcaggg
     1861 cacggcgcag gctgattctg ccttttatct tcagcagatg cagcaaagct ctgatgatac
     1921 caggatcaac tggcaattac tcgccattcg tgcactggtg aaagaaggta aaaccgggca
     1981 ggcggttgag ttgtttaacc aactaccgca agaactgaac gattctcagc gtcgcgagaa
     2041 aacactgctg gcggtagaga ttaaactggc gcagaaagat tttgctggcg cgcaaaactt
     2101 gctggcgaaa atcacacctg ccgatttaga acaaaaccag caagcgcgtt actggcaggc
     2161 aaaaatcgat gccagccagg ggcgtccttc cattgattta ctgcgcgcgt taattgctca
     2221 ggaaccgctg ctcggcgcga aagaaaaaaa gcagaatatt gatgccacct ggcaggcgct
     2281 ctcctccatg actcaggaac aggcgaatac gctggtgatc aacgccgacg aaaatattct
     2341 gcaaggctgg ctggatctgc agcgcgtctg gtttgataac cgtaacgatc ccgatatgat
     2401 gaaagccggg atcgccgact ggcagaaacg ttatccgaac aatccgggcg cgaaaatgct
     2461 gccaacgcag ttggttaacg taaaagcgtt taaaccagcc tcgaccaaca aaatcgccct
     2521 gctgttgccg ctgaatggcc aggcagcggt atttggtcgc actattcagc aaggctttga
     2581 agcggcgaaa aatatcggca ctcagccagt ggcggctcag gtagctgccg cagacgtagc
     2641 agaacaacct cagccgcaaa ctgcggatgg cgttgccagc ccggcacaag cctcggttag
     2701 cgatctgacc ggtgatcagc ctgcggccca gccggtgcct gtaagcgccc cggcgacaag
     2761 caccgcagcg gtaagcgcac ccgcaaatcc atccgcagag ctgaaaatct acgatacctc
     2821 atcacaacca cttagccaga tcttaagcca ggttcagcag gatggcgcga gtattgtggt
     2881 tggtccgttg ctgaaaaata acgttgaaga gttgcttaag agcaacaccc cgctgaacgt
     2941 gctggtactg aaccagccgg agaatatcga aaaccgcgtc aatatttgtt acttcgcgct
     3001 ttcaccggaa gacgaagcgc gcgatgcagc gcgtcatatt cgtgaccagg gtaaacaagc
     3061 gccgctggtg ctgatcccac gcagttcatt gggcgatcgc gtagccaatg cgtttgcgca
     3121 ggagtggcag aaactgggcg gcggcaccgt tctgcaacaa aaatttggtt ccaccagcga
     3181 attacgcgcg ggtgttaacg gcggttctgg tatcgcttta acgggtaccc cgattactcc
     3241 cagagcgaca accgactccg gcatgacgac caacaatcca acgctgcaaa ccatgccaac
     3301 cgatgaccag ttcaccaata atggcggtcg tgtcgatgcg gtgtacattg tggcaacgcc
     3361 gggtgaaatc gcttttatca aaccgatgat cgccatgcgt aacggtagcc agagcggtgc
     3421 aatgctgtac gccagctccc gcagcgcaca aggcaccgca ggcccggatt tccgtctgga
     3481 gatggaaggt ttgcagtaca gcgaaatccc gatgctggcg ggcggtaatc tgccgttaat
     3541 gcagcaggca ctcagcgcgg tgaataacga ttattcactg gctcgcatgt atgcgatggg
     3601 cgtcgatgcc tggtcgctgg caaatcattt ctcacaaatg cgccaggttc agggttttga
     3661 aatcaacggt aataccggaa gcctgacggc aaacccggat tgcgtgatta acaggaagtt
     3721 atcatggcta cagtaccaac aaggtcaggt agtccccgcc agttaaccac caaacagacc
     3781 ggcgatgcgt gggaagtaca agcgcgtcgc tggctggaag gcaaaggact gcgatttgtc
     3841 gccgctaacg tgaacgagcg tggcggtgag atcgatctga taatgcgtga aggctggacc
     3901 accgtttttg tcgaggtgcg ttaccgccgc tctgcgcttt atggcggcgc ggcagccagt
     3961 gtgacccgca gcaaacaaca caaattatta cagactgccc gcttgtggct cgcgcgtcat
     4021 aatgggagtt ttgatactgt ggattgccgg ttcgatgtgg tagccttcac cgggaatgag
     4081 gttgagtgga ttaaggatgc ctttaatgac cactcataat taaggtttaa ggattagcgt
     4141 gcaagaaaga attaaagctt gcttcactga aagcattcaa actcaaattg cggcggcaga
     4201 ggcgcttccg gatgccatct cccgtgcagc catgacgctg gttcagtctc tgctcaatgg
     4261 caacaaaatc ctctgttgtg gtaatggaac ttccgctgcc aatgcacagc attttgctgc
     4321 cagcatgatc aaccgtttcg aaacggagcg gcccagctta cctgccattg cactaaatac
     4381 tgataatgtt gtcttaacgg cgattgccaa cgatcgctta catgatgaag tgtatgcaaa
     4441 acaggtgcgg gcgctgggtc atgcgggaga tgtattgtta gccatttcca cccgtggcaa
     4501 cagccgcgat attgttaaag cagttgaagc cgccgttacg cgtgatatga ccattgtggc
     4561 attgaccggc tatgacggcg gcgaacttgc aggtttgtta gggccacagg atgtggagat
     4621 ccgcattcct tcgcatcgta gtgctcgcat tcaggaaatg catatgctga cggtaaattg
     4681 cctgtgcgat ctgatcgata acacgctttt ccctcaccag gatgattaag gagaatacat
     4741 gaaggcatta tcgccaatcg cagtccttat ttccgcgctg ctgttgcaag gttgtgttgc
     4801 cgctgccgta gtgggtaccg ctgctgtggg taccaaagcc gcaactgacc cacgcagtgt
     4861 cggtacccag gtggacgatg gtaccctgga agtgcgcgtg aacagcgcat tgtcgaaaga
     4921 cgaacagatt aagaaagaaa cccgtattaa tgtcacggcg tatcagggca aagtgctgct
     4981 ggttgggcag tcaccaaatg ctgaactttc ggctcgcgcc aaacagattg ctatgggcgt
     5041 agacggtgcc aacgaagtgt ataacgagat tcgtcagggc cagccgattg gtctgggcga
     5101 agcatctaac gatacgtgga tcaccaccaa agtgcgttcg cagctcttaa ccagcgacct
     5161 ggtgaaatcg tccaacgtga aagtgaccac cgaaaacggt gaagtgttcc tgatggggct
     5221 ggtgactgaa cgtgaagcga aagcggcggc agatattgcc agccgggtga gcggcgtaaa
     5281 gcgggtaacc acagcgttta cgtttattaa atagcagccc cttgtaatgc ctgatgcgac
     5341 gcttgccgcg tcttatcagg cctacaacga tgcaaaccgt aggtcggata aggcgtttac
     5401 gccgcatccg acatgttttc cctcaaatca gaacaacagc gccagcccgc cgacaatcac
     5461 accagacact gccaccatcg cccctgtcag ccataaggct ttcgccggga acgctttgcg
     5521 cagcataatc agtgacggca aactcactgc cgggagcgtc atcaacaatg ccagcgccgg
     5581 agcggttccc atacctgcca gcatcatcgt ttgtacaatc ggaatttctg ctgccgtggg
     5641 aatgacaaac aagcatcctg ctaccgccat cgccaccacc cacatcaggc tgttatcgac
     5701 agcaccatcg gcatggggga ataaccagac gcgtgcggca cccaacacca gtactgcaag
     5761 gatgtaaacc gggatcgtac tccagaaaag tgtccatagc gccctgcccc agcggctaaa
     5821 aaaaccgctc cgtgcttccg gtatgtcaat ttcgaccggt gcctgcgttt gcggtgtttc
     5881 acgcacccat ttttgcacca gcgtcgcaat cagcaacacc atcaccagcc cggccaccag
     5941 acaaatcgcc gcaaaacccc agccgaggac aaaccccata aacaccagcg tcgccgggtt
     6001 taacaccgga ttgcccatcc agaatgccag cgcaccgccc atcgatacct gttggcgacg
     6061 catccccgcc gcgaccggag ccgcacagca ggtacacatc atgcccggca gcgaaaacag
     6121 cgttcccagc agcgtgccgc gaaagcgcga ttgcccaagc gtacgcaaca accagtcacg
     6181 cgggatcagc acctgaatca acgaaccgag gatcaccccc agcaccgccg ctttccatac
     6241 tgcgaggaaa tagatcatcg cgtaatccaa cgccgcttgc cacggattag catccgcctg
     6301 cgcaaggata gatttaccga tactgtgggt ttcggctgca gtaaacgctt tgccgtagta
     6361 atgttgccat ttcacatacc agagaccggc aatgacaacg agaaagaaaa gcgcgggttt
     6421 ccaccactga atgggtgttg ccgcctgaga tgaagactga ccagtcatag cattccctag
     6481 gaataagtta tgataattga gcgcgtgaat attacgctca ctatcaattc ttgggaataa
     6541 ttattcagct cttttgcgta attctgaaga gcttaaaatc gtcacgcctt catgctcagg
     6601 ccgcatcgcc tctgccagca tgacccgcgc cacgtctctc gcatcaatgg atttccagtt
     6661 acctggtaac aaacggaaca gcggcgcaaa aagcgtttcg ttcatccgct gtttgctacg
     6721 atcgcccagt aacatcgacg ggcgagcaat ggtcaatttc ggccagttct gggcaattaa
     6781 tgcttcttcc atctcccctt tgacgcggtt atagaaaaac ggcgagtggg cattggcccc
     6841 catagcactg accaccagca tatgctgcgc gcccagccgc cgcccggtta atgcggtatc
     6901 cactaccagc gtgtaatcgg catgaataaa cgcctctttg ctccccgctt ctcgccgcgt
     6961 ggtgccgaga caacaaaaca caatgtcgat gggttcggtg acctgcgcca acgcgtcact
     7021 cagttgcgga tcatggggat taaacacccc gggcatatcg cccaacggac gtcgcgtcgg
     7081 cgcagcaatg gcgttaactt tcggttcatt aatcaacatc cgcagcaggt gaccgcccac
     7141 cagccccgtt gcgcctgtaa tcagtacctg actcatcttc gctcctttac agaattgtct
     7201 gccttgcgct ccacggctca tgcaccagtc ttaatagacc atgaggtaat tatccccgat
     7261 tgtgggaaat tcgcctcatc caatgcaaca acgcggagga agcatgagta agaaaattgc
     7321 cgttttaatc agtgatgaat ttgaggattc agaatttact tcacccgcag acgagttccg
     7381 taaagccgga cacgaagtaa ttaccattga aaagcaagcg ggtaaaacgg tgaaaggcaa
     7441 aaaaggagaa gccagcgtga ccatcgataa atccatcgat gaagtgacgc cagcggagtt
     7501 tgatgccctg ctgctaccgg gcggccattc accggattat ctgcgtgggg acaaccgttt
     7561 tgtcaccttt acccgtgatt ttgtgaatag cggcaaacct gttttcgcca tctgtcacgg
     7621 tccgcagctg ctgatcagcg ccgatgtgat tcgggggcgc aaactgaccg cagttaaacc
     7681 gatcattatt gatgttaaaa atgcgggcgc ggaattttac gatcaggaag tcgtggttga
     7741 taaagatcag ctggttacca gccggacacc ggacgatctg ccagcgttta accgcgaggc
     7801 gttacgcctg ctcggtgcct gagtcgcgta gccaaatcat ttttttacca aagcccagcg
     7861 tgttgtcggt gaatttgatt tcatcgagcc ggatttccca caccggggcc gacagcattc
     7921 tggcaaccgg aaagcgatga ttgtacgcct tgcgcgcgag gtcgctttcc tcaccttcca
     7981 gcctgcggat ctcaccttta aactgcacac cgcgaattaa cgctaccgtt ttcggctgac
     8041 cgtttaccgt tccggcaacc gcagcctgcg gcccactcat ctgcgcgtgg cgcgtttttt
     8101 cttccgtcaa aatgtagaag gcgactttct gcgcatcaaa cagataaaaa gcattagcgc
     8161 accacagttc cccttcctgc tgcacacacc aggtgacaac atgttgtttt gccaaccaac
     8221 ggctgatggt gatgagtgtt tccattgctg ttctctttta tactgtgggc ctgaccttaa
     8281 catgcgacgc agacgatgac accctggttt ctttacttga tccgtaccgc cgacaataag
     8341 ctttataccg ggatcaccac ggatgtcgaa cgccgctatc agcagcacca aagcggcaaa
     8401 ggggcgaaag cactgcgcgg gaaaggagaa ctgacgctgg cgttttctgc gccagtgggc
     8461 gaccgttcgc tggcactacg ggcggaatat cgcgttaagc aactgaccaa acggcaaaaa
     8521 gagcgtctgg tagcggaagg cgcagggttt gcagaactgt tgagcagcct gcaaaccccg
     8581 gagattaaaa gcgattgaaa tgctcgtgat actcaaccag accggttacg ccattcagcg
     8641 cgtcatccgc taaacgatgt acctggaagg cgctttcagt gcccggccag cggcaacgca
     8701 gatcgtgatg cgccgccagt tcaaagccga aacgactgta cagcgccgga tcgcccagcg
     8761 tcaccactgc ggcatagccg aactcattaa gcgaatcgag tccttcatag accagttggc
     8821 gtgccagccc ctgtccacgg tatttttcat caaccgccaa cggtgccatg ccgacccatt
     8881 gcaggtcttc gccctgcaca tcaaccggac taaatgccac atagccaatg acctgacctt
     8941 cgtcatctgt cgccaccagc cccagcgtca gaaagccatc ttcacgcaga tcgtgaacca
     9001 gcttcgcttc cgcatcactt tcgaatgagc gacgcagcag ggcatcaata cccggcgcat
     9061 caatgggaat ttctactcga attagcatgg ttcacctacc gatgtctgtt tggtttcagg
     9121 cgcggttttc attcccgcct caacaaaatc cgccagttgc agcagcatca tgcgtaaggc
     9181 tttcggcatt tgctccagtt caatggcgtc catcaggttt ttcacatgca gccccagctc
     9241 cgtatcgcct tcaatcacca gccgacgctg gaagaagagc gtatctggat cttgtttacg
     9301 agccgcaatc atcagcagat cgctgacgtc ggcactaaaa ctcacatcag cttgcgcgtt
     9361 ctggctgacc accagtttgc cattcaccac cgaggtaaac cattgcaggt caatatcacg
     9421 cacatgaata cttaaccagc ggccttcaag aaactccagc tcgccatcat ccagcgcctg
     9481 acggaattgc cagcttaaga cctgctcaag aacctggcgt tttagcgcaa atggcgtcag
     9541 ttttaccggt acactcaaca gagatggccc caaatgcaca atacgggaac gaagtttatc
     9601 caacacgagt tttactccct gtttcaacaa tcatcctatt ttgccatatc agaaaaataa
     9661 catagcggta taaatcaaca attccatatg aaattgctgc taccaccaat acaactttaa
     9721 ctgccttaaa tcaaaaattg tcgcagcaag gttaactaaa atcccagttc gttaacattt
     9781 ttgcgttttg atagcgcaac cttcaggaaa aattatggag ctgctctgcc ctgccggaaa
     9841 tctcccggcg cttaaggcgg ccatcgaaaa cggcgcagat gctgtttata tcgggctaaa
     9901 agatgatacc aatgcccgtc acttcgccgg ccttaacttt accgagaaaa aattgcagga
     9961 agcggtgagt tttgtccatc aacatcgccg caaacttcac atcgcgatta acacttttgc
    10021 gcatccggac ggttacgccc gttggcagcg cgccgtggat atggcggcgc agctgggtgc
    10081 cgacgcgctg atcctcgccg acctcgccat gctggaatac gccgccgagc gttatccgaa
    10141 tattgagcgc cacgtatcgg tgcaggcttc ggcgaccaat gaagaggcga ttaactttta
    10201 tcatcgccat tttgacgttg cccgcgtggt gctgccgcgc gtgttgtcga ttcatcaggt
    10261 gaaacagctg gcacgggtca cacctgtacc actggaagtg tttgctttcg gcagcctgtg
    10321 cattatgtcg gaaggccgtt gctatctgtc gtcgtatctg acgggtgagt cgcctaacac
    10381 cgtgggcgcg tgttctccgg cccgtttcgt gcgctggcaa caaacgccgc aggggctgga
    10441 atcccgcctg aacgaagtgc tgatcgaccg ttatcaggac ggcgaaaacg caggttatcc
    10501 gacgctgtgt aaagggcgtt atctggtgga cggcgagcgc tatcacgcgc tggaagaacc
    10561 aaccagtctc aataccctgg aactgctgcc ggagttaatg gcggcgaata ttgcttcggt
    10621 gaaaattgaa ggccgccagc gtagcccggc gtatgtcagc caggtggcga aagtctggcg
    10681 tcaggctatc gaccgttgta aggccgatcc gcaaagcgcg tggatggaga cgctcgggtc
    10741 gatgtccgaa ggcacgcaga ccacccttgg cgcgtatcac cgtaaatggc agtgagaaga
    10801 gcaatgaaat attccttagg gccagtgctg tggtactggc caaaagagac gctggaagat
    10861 ttttatcagc aggccgccac cagcagcgcc gacgtgattt atcttggtga agcggtatgc
    10921 agcaagcgcc gggcaaccaa agttggcgac tggctggaga tggcaaaatc gctcgccgga
    10981 agtggcaagc agattgtgct ctccacgctg gcgctggtgc aggcatcctc tgaactgggc
    11041 gaactgaaac gctatgttga gaacggtgag tttctgattg aagccagcga tctcggcgtg
    11101 gtgaatatgt gcgctgaacg caaactgccg ttcgtcgccg ggcacgcgct gaactgctac
    11161 aacgcggtga cgctgaaaat attgctcaaa cagggcatga tgcgctggtg tatgccggtg
    11221 gagctttccc gcgactggct ggtgaatctg cttaatcagt gcgatgagct gggcattcgc
    11281 aaccagtttg aagtggaagt cctgagctac ggtcatctac cgctggccta ctccgctcgc
    11341 tgctttaccg cgcgttcgga agaccgcccg aaagatgagt gtgaaacctg ctgcattaag
    11401 tatccgaacg ggcgcaacgt gctgtcgcag gaaaaccaac aagtgtttgt actcaatggc
    11461 attcagacca tgagcggcta cgtttacaac ctcggtaacg agctgacatc catgcaggga
    11521 ttggttgatg tggtccgcct gtcaccgcag ggtactgaca ctttcgcgat gctcgacgcc
    11581 ttccgcgcta atgaaaatgg cgctgcgcca ctattgttga cggcaaacag cgactgtaac
    11641 ggctactggc ggcggctggc aggactggag ctgcaagcct aagtaaatat gctcgtcata
    11701 cttcaagttg catgtgctgc atctgcattc gttcacccca gtcacttact tatgtaagct
    11761 cctggggatt cactctcttg tcgccttcct gcaactcgaa ttatttagag cacagctcac
    11821 tttgttaaca actttaacta ctctttaatg cagtattaaa gattaatcgg taacaaagtg
    11881 agctgttatg actgataaaa ccattgcgtt ttcgttactc gatctggccc ccattcccga
    11941 aggttcttca gcgcgagaag cattctccca ctctctcgat ctcgcccgtc tggctgaaaa
    12001 gcgcggctat catcgctact ggctggcaga acaccacaat atgactggca ttgccagtgc
    12061 tgccacgtcg gtattgattg gctatctggc ggcgaatacc accacgctgc atctggggtc
    12121 tggcggcgtg atgttgccta accactcacc gttggtcatt gccgaacagt tcggcacgct
    12181 caatacactc tatccggggc gaatcgattt ggggctgggt cgtgcgccag gtagtgacca
    12241 acggacaatg atggcgctgc gtcgccatat gagcggcgat attgataatt tcccccgcga
    12301 tgtcgcggag ctggtggact ggtttgacgc ccgcgatccc aatccgcatg tgcgcccggt
    12361 accaggctat ggcgagaaaa tccccgtgtg gttgttaggc tccagccttt acagcgcgca
    12421 actggcggcg cagcttggtc tgccgttcgc gtttgcctca cacttcgcgc cggatatgtt
    12481 gttccaggcg ctgcatcttt atcgcagcaa cttcaaaccg tcggcacgac tggaaaaacc
    12541 atacgcgatg gtgtgcatca atattatcgc cgccgacagc aaccgcgatg ccgaattcct
    12601 gtttacctca atgcagcaag cctttgtgaa gctgcgccgc ggcgaaaccg ggcaactgcc
    12661 gccaccgatt caaaatatgg atcagttctg gtcgccgtcc gaacagtatg gtgtgcagca
    12721 ggcgctgagt atgtcgttgg taggtgataa agcgaaagtg cgtcatggct tgcagtcgat
    12781 cctgcgcgaa accgacgccg atgagattat ggtcaacggg cagattttcg accaccaggc
    12841 gcggctgcat tcgtttgagc tggcgatgga tgttaaggaa gagttgttgg gatagtgtgt
    12901 cttaacgcgg gaagccttat ccgagctggc aacgctgtcc tacgtagacc tgataagcga
    12961 agcgcatcag gcattgtgta ggcagcagaa atgtcggata aggcaccgct gattactgat
    13021 acaccggcag taaattaaag ctcgataaaa tatgtaccaa tgcgttgccg acgccaaaca
    13081 ccagaatcag cgtaatcatc ggcttgccgc cccagacgcg gaatttcggg ctgccaaagc
    13141 gtttacgcga tgcgcgggcc agcagtgccg gaacaattgc cgcccagatg gtagccgcta
    13201 aaccagcata accaatggcg tacaggaatc cattcgggaa cagcagacca cccacaacag
    13261 gcggtgcaaa ggtcaacaat gctgttttca agcggcccat agccgagtca tcgaaaccaa
    13321 acagatctgc cagatagtca aacaaaccca gcgttacgcc aaggaacgaa ctcgctaccg
    13381 caaagtttga gaacacgacc agcagaaggt ccaggctacg gctgttcagc acgccgctta
    13441 atgcctgtac cagcacatca atattaccgc ccttctctgc aataccgata aactccggac
    13501 gcgggatgtt acccatcgtc gccagcaacc agatggtata cagcgccagt gccatcagcg
    13561 taccgtacac caggcatttc acgatggttt tcggatcttt gccgtaatac ttcatcaggc
    13621 ttggcacgtt accgtgataa ccaaacgacg ccaggcagaa cggcaatgtc atcagcagat
    13681 acggcgcata agatgcattg ctttctgcaa cgttgaacaa tgtcgcaggc tgcacatgcc
    13741 ccaacaggct gccaaaggtg aggaagaagg taatgacttt cgcccccagc acaatcgccg
    13801 tcatgcggct gacggcctta gtactcaacc acaccacaaa cgctaccagc agtgcaaaac
    13861 caaaacccgc cgcccgtgcc gggacgttta gcgacatctc tgcgaaggtg tgatgcagaa
    13921 ttgaaccact ggcagaaata taggcatagg tcaggatata gagcacaaag gcaatggaaa
    13981 taccgttgac cacgttccag cctttgccca gcaaatcttt ggtgatggtg tcaaaactcg
    14041 aaccgattcg gtaattcagg ttagcttcca gaatcatcaa gccggaatgc agcatacaga
    14101 accaggtaaa gatcagcgcc gccattgacc agaaaaacca cgccccggac atgaccactg
    14161 gcagagaaaa catccctgcg ccaataatgg tgccgccgat aatcaccacg ccgccaagca
    14221 gcgacggtga cgtttgggtg gtggttagtg ttgccatgag ggcttctctc cagtgaaaaa
    14281 tagtgcgact gcgttgttat gcattgcact gtaccagtac gcgagtacaa aagacagaaa
    14341 aaaagccccg atggtaaaaa tcggggctgt atatattatt ttacagattg tgttcgctgt
    14401 tcagcgatga ttacgcatca ccaccgaaac gacgacgacc ggtagagtca tcacgacgcg
    14461 gagcgcggcc ttcacgacgt tcgccgctaa aacgacgacc atcaccacgg ccaccttcac
    14521 ggcgttcacc gctgaagtta cgaccgcctt cacgacgttc gccaccgaaa ccacgaccac
    14581 cgccacgacg ctcaccgcca gtatgcggct gtgcatcgcc cagtaactgc atgttcatcg
    14641 gcttgttgag aatgcgagtg cgcgtaaagt gttgcagcac ttcacccggc atacctttcg
    14701 gcagttcgat ggtggagtga gaagcaaaca gcttgatgtt accaatgtaa cggctgctga
    14761 tgtcgccttc gttagcaatc gcaccaacga tatgacgaac ttcaacacca tcatcacggc
    14821 ccacttcaat gcggtacagc tgcatatcgc caacatcacg acgttcacga cgcggacgat
    14881 cttcacggtc accacgcggg ccacggtcgt tacgatcgcg cggaccacgg tcatcacggt
    14941 cacggaattc acgtttcgga cgcatcggcg catctggcgg tacgatcaga gtacgttcac
    15001 cctgtgccat tttcagcagt gccgcagcca gagtttcgag atccagctct tcaccttctg
    15061 cagtcggctg aattttgctc agcagtgcgc ggtattgatc cagatcgctg ctttccagct
    15121 gctgctgtac tttagcggcg aatttttcca gacggcgttt gcccagcagt tctgcgttcg
    15181 gcagttctac ttccggaata gtcagcttca tagtacgttc aatgttgcgc agcagacgac
    15241 gctcgcggtt ctcaacgaac agcagcgcgc ggccagcacg acccgcacga ccggtacgac
    15301 cgatacggtg aacgtaagac tcagaatcca tcgggatatc gtagttaact accaggctga
    15361 tacgctcaac gtccaggcca cgggctgcaa cgtcggtcgc aatcaggatg tccagacgac
    15421 catctttcag gcgttccagt gtctgttcac gcagcgcctg gttcatgtca ccgttcagcg
    15481 cggcgctgtt gtagccgtta cgctcaagag cttcagccac ttccagagtc gcgtttttgg
    15541 tacgaacgaa gataatcgcc gcatcaaaat cttccgcttc caggaaacgt accagtgctt
    15601 cgtttttgcg cataccccag acagtccagt agctctggct gatgtcagga cgggtagtca
    15661 cgctggactg aatgcgcact tcctgcggct ctttcataaa gcggcgggta atgcgacgaa
    15721 tcgcttccgg catggttgca gagaacagag cggtctgatg accttccggg atctgcgcca
    15781 taatggtttc aacgtcttcg atgaagccca tgcgcagcat ttcgtcagct tcatccagaa
    15841 ccagaccgct cagtttagag aggtccagag tgccacgttt caggtggtcc agcagacggc
    15901 ccggagtacc gacaacgatc tgcggcccct gacgcagggc gcgtaattgc acgtcataac
    15961 gctggccgcc gtacagagca accacattta cgccgcgcat gtgtttagag aaatccgtca
    16021 ttgcttcagc aacctgtacc gccagttcgc gggtcggtgc cagcaccaga atctgtggtg
    16081 ctttcagctc aggatcaaga ttctgcaaca gaggtaaaga gaatgctgca gtttttccgc
    16141 tccccgtctg ggccataccc agaacgtcgc ggccattcag cagatgtgga atacactctg
    16201 cctgaattgg agatggtttt tcgtaaccca gatcgttaag ggcttcaagg ataggagcct
    16261 tcaggcccag atctgcaaaa gtggtttcga attcagccat gtagtacgtg tgcctcaaaa
    16321 ttaatggcgg ccagtctaca taactcatca tgaaattgat cagcaatttt cattgaaaag
    16381 tgtgaaccgg ctcaaagtag gtgtattaac gaacaacaac gccctcaccc cttaaggtga
    16441 tggcaatcaa aaaagattac gggctgatgt gtacgtcagc tattgctggt ccgattctgc
    16501 caggtcatct tggtcctggc ccaggagcga taattccaac aatgcgtatc ggtgctcaac
    16561 aaagttatga acgttgttgg caaccgccag tttgaacagt gccgtggcgc tgtccgaatc
    16621 ccccagactt aggtagtact tacctaaata gaagttggtt tcactgagat gctcagcgag
    16681 cgaggtgtta tccgttgcgt ccgccttgag cctttccatt aacgtttgtt cgctaatgtt
    16741 gcccaggtag aactcgacaa tgttccatcc ccactgttcc ttatccgatt tttcgaagtg
    16801 ctgtttcaac acttctttag cctgcttctc atcgagcttc tgctcggcga gataaagcca
    16861 cagactacgg aaaggatcat tgggatcgtc ttgataaaac gccagcagat catcttgcgc
    16921 taacttgtca cgaccgccgt aatataatgc gatcccgcga ttcaagtgcg cgtagttgta
    16981 agttggatca agctcaagta cagaatcaaa cgcttcatag gcagcatcaa aattgcctgc
    17041 ctgcgttaaa tatatgccta agtaattgaa cacttcaggc atatccggtc ggattgccag
    17101 cgcttgcgaa aaatcgttac gcgctaatgc cctcagaccg agactatcat acaacactcc
    17161 gcgctcatat aaaagctgtg cgcgttcgtc atcggttaaa gcccgactgg caaggatttg
    17221 ttccatacgt gccagaatca cttcctgctg taaagtcggt tgcaatggta ccgcgaggac
    17281 ttcactttta cgccaggaag tattactgca tcctgcaagc gtaagtgctg tcgcaacgaa
    17341 acaccagcgc aaaaaaggct tcatttccca ctcccgaaga ccacggttga atgaacgtcc
    17401 tgttcccggt tgctaacaag gcgtcctgcc cggttaaaag ccccccgccg cagcggaggg
    17461 caaatggcaa ccttactcgc cctgttcagc agccggagct tccggtgctg cagcaggttg
    17521 agactgctca gtcgcttctt taatgctcag acggatacgg ccctggcgat caacttccag
    17581 aactttcacc ggtacttcct gacccatctg caggtaatcg gtcactttct caacgcgttt
    17641 gtcagcgatt tgagagatgt ggaccagacc ttctttaccg ccgccgatgg caacaaatgc
    17701 gccaaagtca acgatacggg tcactttacc agtgtagacg cggcccactt cgatttctgc
    17761 agtgatctct tcgatacgac gaatagcatg tttcgctttc tcgccgtcgg tcgctgcgat
    17821 cttcacagta ccgtcatctt cgatttcgat ggtagtgcca gtttcttcgg tcagggcacg
    17881 gataacagag ccgcctttac cgataacgtc tttgatcttg tccgggttga tcttgatggt
    17941 atggatacgc ggtgcgaact cagagatatc gccacgcggc gcgttgatcg cctgttccat
    18001 tacgcccagg atatgcagac gcgcaccttt agcctggttc agcgcaacct gcatgatctc
    18061 tttggtgata ccttcaattt tgatatccat ctgcaatgca gagataccct cgcgggaacc
    18121 cgcaactttg aagtccatat cgcccaggtg atcttcgtcg cccaaaatgt cagacagtac
    18181 aacgtagttg tcgccttctt tcaccagacc cattgcgata cccgcaacgg cagctttgat
    18241 cggcacacct gcgtccatca gcgccagaga cgcgccgcac acggaagcca tagaagagga
    18301 accgttggat tcagtgattt cagacacaac acgtacggtg tacgggaatt tgtccatatc
    18361 cggcatgact gccagcacgc tgcgcttcgc cagacgaccg tgaccaattt cacgacgctt
    18421 cggagaaccg accatgccgg tttcgcctac ggagtacgga gggaagttgt agtggaacag
    18481 gaaggtatcg gtacgttcgc ccatcagttc atcaagaacc tgcgcgtcac gagcagtacc
    18541 cagcgttgcg gtaaccagcg cctgcgtttc accacgggtg aacagcgcag aaccgtgagt
    18601 acgcggcagc acgccagtac gcacatccag accacggatc atatcttttt cacgaccgtc
    18661 gatacgcggt tcgcctgcca gtacgcggct acgaacaacg tttttctcga tagcgtgcag
    18721 aatttcaccc agttcgtttt cgtccagggt ttcgtcttca gcaagcagcg tcgcgatggt
    18781 ttcagatttg atgacatcaa cctgcgcata acgctcttgt ttgtcggtga tgcggtaagc
    18841 atcgctcagg cgagcttcag ccagtgcagc aacgcgcgcg ttcagcgctt cgtttaccgg
    18901 ctccggctgc cagtcccaac gtggtttacc ggcttctttc accagttcat tgatgttctg
    18961 aataacaacc tgctgttgtt catgaccgaa cactactgcg cccagcatct ggtcttcgct
    19021 cagcagttca gcttcagatt caaccatcag aacagcggct tcagtaccgg caacaaccag
    19081 atccagtttg ctctctttca gctcgtcctg agtcgggttc agtacgtact ggtcattgat
    19141 gtaacctacg cgagcagcac caatcggacc attgaacgga ataccagaca gagacagtgc
    19201 tgcggaagca ccaatcatcg cgacgatatc cgggttaact tgcgggttaa cagaaaccac
    19261 ggtggcgata acctgaactt cgttgacgaa gccttccggg aacagcgggc gaatcgggcg
    19321 gtcaatcaga cgcgcgatca gggtttcgcc ttcgcttgga cgaccttcac gacggaagaa
    19381 gctacccggg atacgaccag cagcgtaggt acgctcctga tagttaacgg tcagtgggaa
    19441 gaagtcctga cctggtttgg cttttttctg gccaacaacg gtaacaaata ccgcggtgtc
    19501 atccatgcta accataacag cggcagtagc ctgacgagcc atcatgccgg tttccagagt
    19561 cacggtgtgt tggccgtact ggaatttacg aacgatcgga ttaagcaatg taatatcctt
    19621 tctctttctt agacagtacc ttacggcact ggtgttaata cccgatcttc tgcgcatcct
    19681 cgcgactaat gacaacccta acccagctct atgtgggtaa agcctctcat tagccgcgcg
    19741 aacctctgca acggaagatc attcatagca acaatacatt agtttccagt gaattgctgc
    19801 cgtcagcttg aaaaaagggg ccactcaggc ccccttttct gaaactcggc aggaattagc
    19861 gacgcagacc caggcgctcg atgagctggg tgtaacgtgc tacgtcttta cgtttcaggt
    19921 agtcgagcag tttacgacgc tgagaaacca tgcgcagcag accacgacgg ctgtggtgat
    19981 cttttttgtg ctctgcaaag tggccctgca ggtggttgat ctgtgcagtc agcagtgcta
    20041 cctgaacttc ggtagaaccg gtgtcgtttg cgtcacgacc aaactcagaa acgattttag
    20101 ctgttgcttc agtacttaga gacattttaa aactccaaag tatatagaat gaaaggacgc
    20161 cgatctctaa ttcagcgatc ccagtgtacg ttacgcaaag tgttaaacaa tttacgcgac
    20221 gttaagcggc agtattctac tcgtagcgac ttgttatcgc aagacggtta acattacgcc
    20281 gggtattcaa ccaccaggcg acgaggcgcg acgcggcctt catcgtcaat ttcgcccata
    20341 ccgataaact tgccgttctc accttctgta acgcgaacca gtccttccag cggcgcaccg
    20401 gatgtacgaa ctgggttacc atttttgaag taaacagaag acgttaacgg aagattcacc
    20461 accgggtagt ccgaagctgg actgtccatt ggcatcagta atggatcaag taactccgca
    20521 gccggaatat cctgctgttc ggcttgttcg accagttcac gcagatgttc cagggtcacc
    20581 atccgttcaa ccggatattt acttaccgcc agacgacgca ggtaaataac atgcgcgcca
    20641 caaccgagtt tttcacccag gtcatcaatg atggtgcgga tataagtgcc ttttgagcag
    20701 tgaatttcca gctccagctc attgccttcg tggcgaataa acagcaattc ataaatggta
    20761 atcgggcgcg cttcacgcgg tacttcaatg ccctgacgcg catattcgta cagtttttta
    20821 ccctgatatt tcagtgctga atacatcgaa gggatctgtt cgatatcgcc acggaaagta
    20881 tccagcgccg ccgccagttg ctctgcgcta aaggttaccg gacgttcttc aacgatttgt
    20941 ccgtcggcat cagaagtatc ggtacgctgt ccaagacgcg caatgacccg atagcgtttg
    21001 tcggagtcca gcagatactg ggaaaacttc gtcgcttccc cgaagcaaat tggcaacatg
    21061 ccagttgcca gcgggtccag cgcaccggta tgcccggcac ggttggcgtt ataaatccgt
    21121 ttcacttttt gcagcgcatc attgctggac ataccctgcg gtttatccag caacaaaacg
    21181 ccgttaatgt cgcgaccgcg acgacgagga cgactcatta gtcctccttg ctgtcgtccg
    21241 ggttaacacg acgttcttcg tcatgtttga ccacgctggt caccaggttt gacatgcgca
    21301 tcccttcaac cagagagttg tcgtagaaga aggtcagttc cggcacgata cgcaggcgca
    21361 tcgctttccc cagcaggctg cggatgaaac cagaagcttc ttgcaacgct ttgatgcccg
    21421 ctttaaccgc gtcttcatct ttgtcgttga ggaacgtcac atatactttg gcatacgcca
    21481 ggtcgcgaga catttcgaca ccggaaacgg tggtcatcat gcccaggcga ggatctttaa
    21541 tttcacgctg caggatgaga gcgatctctt tttgcatttc ctgcgctacg cgctgcgggc
    21601 gaccaaattc tttcgccata ataaattctc ctgacaaaaa agggactgtt agcccctttt
    21661 taaaattaat ttcaggtgga agggctgttc acgttggcct gataagacgc gccagcgtca
    21721 catcaggcaa tccatgccgg atgcggcgta aacgccttat cccgcatgga accctaaaaa
    21781 ccttaagcga tggtacgttg gatctcgatg atttcgaata cttcgatcac atcgccagtg
    21841 cggacgtcgt tgtagttctt aacgccgata ccacattcca taccgttacg gacttcgtta
    21901 acgtcatctt tgaagcggcg cagggactcc agctcgcctt cgtagataac cacgttgtca
    21961 cgcagaacgc ggatcgggtt gtgacgttta accacacctt cggtaaccat acagcctgcg
    22021 atggcaccaa atttcggcga tttgaacacg tcacgaactt ccgccagacc gataatctgc
    22081 tgtttcagtt ccggagacag cataccgctc atcgccgctt tcacttcgtc aatcaggtta
    22141 tagatgacgg agtagtaacg cagatctagg ctttccgctt caatcacttt acgtgcagag
    22201 gcatcagcac gtacgttgaa gccaaccagg atggcgttgg acgccgcagc cagggtggcg
    22261 tcggtttcgg tgataccacc tacgccagaa ccgatgatct tcactttaac ttcgtcagta
    22321 gacagtttca gcaaggagtc ggagatcgct tccacagaac cctgtacgtc tgccttcagg
    22381 acgatgttca cttcgtgaac ttcgccttcg gtcatgttgg cgaacatgtt ctcgagttta
    22441 gatttctgct gacgcgccag tttaacttcg cggaatttac cctgacgata cagtgcaact
    22501 tcacgcgctt tcttctcgtc acgtacaacg gtaacttcat cacccgcagc cggtacgccg
    22561 gacaggccga ggatttccac cggaatggac ggacccgctt ccagcacttc ctgacccagt
    22621 tcgttacgca tcgcacgaac acgaccgtat tcgaagccac acagaacgat atcgcccttg
    22681 tgcagagtac cttcacgtac cagaacggtt gcaaccggac cacgaccttt atcgaggaag
    22741 gattcgataa ccgcaccgct cgccatacct ttacgtaccg ctttcagctc cagaacttcc
    22801 gcctgcagca ggatagcgtc cagcagttca tcgatgccgg tacccgcttt cgcagatacg
    22861 tgtacgaact ggctttcacc gccccactct tctggcagga tgccatactg ggagagttcg
    22921 ttcttaacac gatccggatc ggcttctggt ttatcaatct tgttcactgc aaccaccacc
    22981 ggtacctgcg ccgctttcgc gtgctggatt gcttcgatgg tctgcggcat cacgccgtcg
    23041 tcggcagcaa caaccaggac tacgatgtcc gttgcctgcg caccacgagc acgcattgaa
    23101 gtaaacgcgg cgtgccctgg ggtgtccagg aaggtgatca tgccgttttc agtttcaacg
    23161 tggtatgcac caatgtgctg ggtaatgccg cccgcttcgc cagaggccac tttcgttgaa
    23221 cgaatgtagt ccagcagaga ggttttaccg tggtcaacgt gacccatgat ggtcacaacc
    23281 ggcgcgcgcg gttcagccgc agcccccgtg tcacggtcgc tcattaccgc ctcttccagc
    23341 tcgttttcac gacgcaggat aactttgtgg cccatctctt cagcaaccag ctgtgcggtt
    23401 tcctgatcga taacctggtt gatggttgcc attgcgccca gtttcatcat cgctttgatg
    23461 acctgagagc ctttaaccgc catcttgttc gccagttcgc caacggtgat ggtttcgccg
    23521 atcacaacgt cacggttaac ggcctgagct ggcttctgga agccttgctg cagggaagaa
    23581 cctttacgtt ttccgccttt acctccacgt actgctgcgc gagcttcttc acgatcagct
    23641 tttgattcag cgtgtttgtt gcctttcttc ggacgcgctg ctttcgcgtt acgaccacgg
    23701 ccacggccgc cttcgacttc acgatcgctt tcgtcttctg cctggcgagc atgttgagaa
    23761 gtagtgacgt gataatcgct ggaatcttca gtcggttccg cgttatcagt ccatttgttt
    23821 tcttccgcca tacgacgtgc ttcttcagca acgcgacgtg cttcttcttc gagtttacga
    23881 cgcgcttctt cttcagcttt acgcttgagc tctgcagctt cctgctcacg gcgggctttt
    23941 tcagcctggg cgtttttagt catatcgtct tgttgattgc tcactttgtc tttttccgca
    24001 gcttcacgtt tcgcttgttc agcagcttca cgcttagctt gttctgcggc ctcacgttca
    24061 gctttttgtt gcgcctcgcg tttagccgat tcttctgcct cacgacgggc ttgctcttcc
    24121 gctgcaaggc gttcagcctc ttgcggatcg cgtttcacaa aggtgcgttt cttgcggact
    24181 tcgatttgta ccgatttgct ttttccaccg gtaccaggaa tgttaagggt gctgcgtgtt
    24241 ttacgttgca gcgtcaattt gtccgggcct gaatttttct gattcaggtg gtcaatcaaa
    24301 gtctgtttct cttgtgcgga cacagagtcg tcagcagact tccggatacc tgcatcagca
    24361 aattgctgta ccaggcgttc cacggaggtc tgtcgctctg cggccagcgt tttaatcgtt
    24421 acatctgtca tgctgttcct tcctgctaca gtttattacg cttcgtcacc gaaccagcaa
    24481 atattacggg cagccataat cagtgctccg gctttttcgt cggtcacccc ttcgatatca
    24541 gccagatcat caatgccctg ttcggcgaga tcttccagcg tacaaacgcc acgggcggcc
    24601 agtttgaatg ccaaatcacg atctatccct tcaaggttca gcagatcgtc agccggtttg
    24661 ttatcaccga ggctttcttc ctgggcctgt gcaatggtgg ccagtgcatt tttagcacgc
    24721 tcgcgcagtg cttcaacggt cggctcatca aggccttcga tttccaacag ctctttcatc
    24781 ggcacatagg ccagttcttc cagcgtcgag aagccttctt ctaccagaac agtcgcgaag
    24841 tcttcgtcga tgtcgagata tttggtgaag gtgtcgatcg ctgcgtgcgc ttccgcctga
    24901 tgcttagcct gcaggtcgtc aacggtcatc acgttgagtt cccaaccgct cagctgcgaa
    24961 gccagacgca cgttctgacc gttacggcca atcgcctgcg ccaggttacc ggcttcaacg
    25021 gcgatatcca tggtgtgttt atcttcatcc accacgatag aagcaacgtc tgccggtgcc
    25081 attgcgttaa tcacaaactg cgccgggtta tcatcccaca ggacgatatc gatacgctcg
    25141 ccgcccagtt cagtagaaac cgcctgaaca cgcgcgccac gcatacctac acaggcacct
    25201 accggatcga tacgtttatc gttggttttc accgcgattt tcgcacgaga acccggatcg
    25261 cgagccgctg ctttaatttc aatcacttct tcgccgattt ctggcacttc aatacggaac
    25321 agttcgatca gcatttccgg cttggaacga gtgacgaaca gttgcgcgcc acgcgcttcc
    25381 gggcgaacgg aatagagcac gccacgaacg cggtcgccag ggcggaagtt ttcacgcggc
    25441 agcatatctt cgcgcaggat cacggcttca gcattgttgc ccagctccag agagatgttg
    25501 tcgcggttta cttttttcac cacgccggtg atgatttcac cttcgtgttc acggaactga
    25561 tcaaccacca tcgcacgttc ggcttcacgc actttctgca cgataacctg ttttgccgtc
    25621 tgggtagtga tacggtcaaa ggtaacagac tcaatctgat cttcaacgta atcgcccagg
    25681 ttcaggcttt catcttcata acgtgcggct tcaagggtga tttccttggt cggctgggtg
    25741 acttcatcaa caactaacca gcgacggaag gtgtcaaaat caccgctttt gcgatcgatc
    25801 tgtacgcgga cgtcgatctc ttgttcatat tttttctttg ttgctgtcgc cagcgcgctt
    25861 tccaatgctt cgaaaatctt ctcgcgaggt agcgcctttt cattggatac ggcttcaact
    25921 acagccaaaa tttctttgtt catcgcgggc ttttcacctc atccagacta ttaaaagtgg
    25981 ggaaccaggt tcgccttctg gatattactc agcgcgaaca cttcatcttt accttcgacg
    26041 gtaactgtga tcatttcacc gtctaccgct ttgataacgc cctgccattt acgacggttt
    26101 tgtaccgcca tacggagaac cagagtcacc tcttctccga caaaacgggc gtagtgttca
    26161 gccgtgaaca gtgggcgatc gagacccggt gaggagactt ccaggttata agcaacggtg
    26221 atgggatctt caacatccag cacagcactt acctggtggc tcacatcagc acaatcatca
    26281 acattgatgc catcttcact atcaatatag atgcgcagtg tggatgtgtg accgcgaata
    26341 aattcgatgc caaccagttc aaaacccagg gcctcaactg gcgcagtaat catctctgtt
    26401 aatttttgct ctaatgtgga caagcccacc cccaagacat aaaaaaaggg cctaaagccc
    26461 agttattctg tagtcagata acaaaaaacc ccgataaatc ggggctttat ataactgaac
    26521 cctataatcg caactgcggt ctggagcact ttccagaagg attttttcaa atcccactac
    26581 gaaggccgaa gtcttcacag tatatttgaa aaaggactct aagggaaagt ggttgcgggg
    26641 gccggatttg aaccgacgac cttcgggtta tgagcccgac gagctaccag gctgctccac
    26701 cccgcgcctg aaactcggca aattctactc gttttgggta aaagatgcaa atactgctgg
    26761 gatttggtgt accgagacgg gacgtaaaat ctgcaggcat tatagtgatc catgccacat
    26821 tttgtcaacg tttattgcta atcacgtgaa tgaatatcca gttcactttc atttgttgaa
    26881 tacttttgcc ttctcctgct ctcccttaag cgcattattt tacaaaaaac acactaaact
    26941 cttcctgtct ccgataaaag atgattaaat gaaaactcat ttattttgca taaaaattca
    27001 gtgagagcgg aaatccaggc tcatcatcag ttaattaagc agggtgttat tttatgacga
    27061 cgattctcaa gcatctcccg gtaggtcaac gtattggtat cgctttttct ggcggtctgg
    27121 acaccagtgc cgcactgctg tggatgcgac aaaagggagc ggttccttat gcatatactg
    27181 caaacctagg ccagccagac gaagaggatt atgatgcgat ccctcgtcgt gccatggaat
    27241 acggcgcgga gaacgcacgt ctgatcgact gccgcaaaca actggtggcc gaaggtattg
    27301 ccgctattca gtgtggcgca tttcataaca ccaccggcgg cctgacctat ttcaacacga
    27361 cgccgctggg ccgcgccgtg actggtacca tgctggttgc tgcgatgaaa gaagatggcg
    27421 tgaatatctg gggtgacggt agcacctata aaggaaacga tatcgaacgt ttctatcgtt
    27481 atggtctgct gaccaatgct gaactgcaga tttacaaacc gtggcttgat actgacttta
    27541 ttgatgaact gggcggccgt catgagatgt ctgaatttat gattgcctgc ggtttcgact
    27601 acaaaatgtc tgtcgaaaaa gcctactcca cagactccaa catgcttggt gcaacgcatg
    27661 aagcgaagga tctggaatac ctcaactcca gcgtcaaaat cgtcaacccg attatgggcg
    27721 tgaaattctg ggatgagagc gtgaagatcc cggcagaaga agtcacagta cgctttgagc
    27781 aaggtcatcc ggtggcgctg aacggtaaaa cctttagcaa cgacgtagaa atgatgctgg
    27841 aagctaaccg catcggcggt cgtcacggcc tgggcatgag cgaccagatt gagaaccgta
    27901 tcatcgaagc gaaaagccgt ggtatttacg aagctccggg gatggcactg ctgcacattg
    27961 cgtatgaacg tctgttgacc ggtattcaca acgaagacac cattgagcag tatcacgcgc
    28021 atggtcgtca gttgggccgt ctactgtacc aggggcgttg gtttgactcc caggcgctga
    28081 tgctgcgtga ctctctgcaa cgctgggttg ccagccagat cactggtgaa gttaccctgg
    28141 agctgcgccg tgggaacgat tattcaatcc tgaataccgt ctcagagaac ctgacctaca
    28201 agccagagcg tctgacgatg gaaaaaggcg actcggtgtt ctcgccagat gatcgtattg
    28261 gtcaattgac catgcgtaat ctggatatca ctgatacccg agagaaactt tttggttatg
    28321 ccaaaactgg cctgctctcc tcctctgcca cttcaggcgt gccgcaggtg gagaatatgg
    28381 aaaacaaagg ccagtaattc gacatcaacc ctgcccttca gggggggggt taccttgctt
    28441 atcctctgta aactcctttc tgaacatcac ataatcgaaa acatcatggt ttgcatccac
    28501 aacccgtgag tctcctttat agtgtgcaat cacttcctca agcgtttgcg gctgttccgg
    28561 ctttgttacc cccatccacg cattaataag gtaattattg taagctgtcg aaaagatgtt
    28621 gttttccgtt gtgcgatcca cgccatcccc aagaacgggg ctataccaga taaacatcgg
    28681 gacatgatat gcctgctggc tagcctccct gcctccatga aaatagacgt tcttcttcgt
    28741 agggtcacgt tccaggccgt ggtcggcaaa atacatgacc gaggcgcggc gatcttttaa
    28801 taattcaaaa acctgaccta gcaaactatc tgtgtaatga atggagttgt catagcaggc
    28861 atcctgatcg tcctgcggtt gaaacacggc gctggattgc ggataggcgc tacaagccgg
    28921 ttcatggctt ccatttaaat gaagaacaat cagtttcttt tgctgcgtat tttgctgtaa
    28981 tgcttgcgat aaatgcggca acaacaattc atcaaatcct ctgacataaa ctgtttccat
    29041 ggcgcgcatg gcgatactgg taactgcagt accattctgc cgaaaagcgg attgcgagct
    29101 tagccagaaa gtctgaaatc ctgcttgatt agccatatta ataatgttgt cggggtaatt
    29161 atgaatgtca tgactcaaaa cagaatcagc agttaaagaa aggggaaccg acagcgcagt
    29221 gtaaggtgcg ctgcttattg cttgattaaa cagtttgatc tgttttcttt gtgcttcaac
    29281 ttgcggtgtc gtagagcgtg tatatccata caaagacata ttgtcgacac gtacagactc
    29341 ccccacaatc aacacgtagg tatcaatacc tgtatccctg actgataatt gaaaatacgg
    29401 caccgtgttt gcaattgaga gtaatctttg atgctctttc gctgctaaag caaaatagtt
    29461 gagattgaaa aacggcgtat aggtagcaaa tcgcgacgct agtatatatg gactgaacgt
    29521 ttttttattt tttgcatctt tataagcaaa ttgacaagcg gaaaataaac tgcccgaaat
    29581 gacaatcagc aataatattc ctgtcacttt ttttgtcggc aaggaaacat catattttat
    29641 tattactgcc caaaaaagaa gggataaaaa agcaaaggca catagataag ggatatacat
    29701 ccccagcatt ttgacaactt catccggatc actctggagc acactaatcg caaatccatc
    29761 attgaaagtt gtaccaaagg tacaccatga atagaggcta atactcatgt ctgccgcagt
    29821 cagtaacaca aatggtgcgg caacaagcag gcgaagcgga agggatgata tacgttttac
    29881 aaccaggatg gtcaggacga agaacagcaa tcgctgaacc atatgcgcgc cggaagaggc
    29941 gactaagttc gtaataccaa aaacaataac acaaagatac aacaaccaat gagatttaaa
    30001 acttctagca aatttgttga atactgtcat ccagaaatcc aagttcacaa ccaatataaa
    30061 caattatcaa caatgttcaa ttttatatca gacatgtttt tcaactctga tccatatcag
    30121 accgaaacac tgttagtttt ttctttttaa atgatgcggc cattgcgttc tcagtaatta
    30181 aataacatta tcaatgcgtc tctaaacatt acgcagaacg tagccatcag tagttatact
    30241 gacaggagca ggatggttta tcgagaagta aacatatccg tattcgtacc ggaactggag
    30301 ttgacacaat aaagtgccaa ttatgtcagc agaagggaaa agtttataac taaggcgtac
    30361 cggcaccatc gtttcaaggt accagctacg agtaaagcaa ctggacgaga tacagatacc
    30421 tgacaaccat tcctcagacc aggaccaaaa cgaaaaaaga cgcttttcag cgtctctttt
    30481 ctggagtttg gtaccgagga cgggacttga acccgtaagc cctattgggc actaccacct
    30541 caaggtagcg tgtctaccaa ttccaccacc tcggcacgga tactactttt tagttcggga
    30601 tatcgctggt cggcttagcc ggagcagctg gttgagtttg ttcggttttc gccggtgcac
    30661 tcagattttc ccattcgcta cctttattgg ttttgttgct gttgatgtta cccagcacca
    30721 gactgatgat gaagaataac gttgccagca gcgccgtcat gcgggtcatg aagttaccag
    30781 aaccacttga accaaacagc gtagcggaag cgcctgctcc gaaggaggct cccatatcag
    30841 cgcctttacc ttgctgcagc atgatcagac caacaaggcc aattgccaca ataaggaaaa
    30901 ctaccaaaag agcttcgtac ataatcaacc tgttccttgc ggagttgccg cgtaccaatg
    30961 cttcaaccaa taaagcggga gtttttaatc tttcccactg aagcgggtgt gaatactaac
    31021 caaagcgaat gaccttcgca agggcaattt tatcgcattg tatcaactgc ggaaaaaaac
    31081 agcaaaaccg atgtgttcgc tgagaaaaaa ggcgacaatc gccgcctttt tagccagtca
    31141 tttatcgttt taaacggctt ttactgcatc ggcgatgcgg tgtgcaaatt cagtcacctg
    31201 cgcttcgtct tcgccttcca ccatcacgcg aattaacggt tcggtgccgg atttacgcag
    31261 caacacgcgt ccacggttgc ccagcgcagc ttcaacctct gcggtcacgg ctttaactga
    31321 ctcatgctca agcggatcgc cgctacctgc ggtgtaacgc acgttaacca gaatctgcgg
    31381 gaacattttc atgccgctgc aaaggtcgtg caggctcata tggttacgtg ccatcgccgc
    31441 cagcacctgc aagccagcaa cgatgccgtc accggtagtg gttttatcca gcaggatcac
    31501 atgaccggaa ttctctgcac cgatacgcca gcctttctcc tgcatttttt ccagtacgta
    31561 gcggtcaccc actttcgcgc gcgcaaatgg aatacctaac tgtttcagcg ccagctcaag
    31621 ccccatgtta ctcatcaatg tacccacagc accaccacgc agctggccct gacgaagacc
    31681 ttcacgcgcg atgatataca tgatctgatc accatcgact ttattgcctt catggtcaac
    31741 cataatcacg cgatcgccgt cgccgtcgaa ggcaataccg agatccgctt tttcagccag
    31801 cacacgagcc tggagcgcgc gaacgtcggt agcccccact tcggcattga tgtttacacc
    31861 gttcggctca caaccgatag cgataacgtt cgcccccagt tcacgcagca cgttcggcgc
    31921 gatgtgataa gtcgcaccgt ttgcgcaatc caccacaatc ttcagttcgc tgaggctaag
    31981 ttcgttcggg aacgtggctt tgcaaaactc gatatagcga cccgcagcat caacgatacg
    32041 gctggcttta cccagttctg ccgaatcaac acagctgatc tctttttcca tttccgcttc
    32101 gatggcctct tctaccgcat ccggtagttt ggtgccgtcg atagagaaaa atttaatgcc
    32161 attatcgtag aacgggttat gcgatgcaga tatcacaatt ccggcctctg cgcggaaggt
    32221 acgcgtcaga taagccacgg ccggtgttgg catcgggcca gtgaagagtg cggaaaggcc
    32281 cgctgccgcc agacccgctt ccagtgctga ctccagcata tagccagaaa tacgcgtgtc
    32341 tttactaata atgatcttac gggagccgtg gcgcgccagc actttacccg cggcccaacc
    32401 cagcttaagc acaaaatcag gtgtgatcgg cgcatcccct acacgaccac gaatcccatc
    32461 ggtaccgaaa tatttacgat tactcatagc gtttgttttc ctttgcagac agagtggctt
    32521 ccaccacccg catcgcttct acggtttctt tgacgtcatg aacacgaata atgtgcgcgc
    32581 cttgcattgc ggcaatgacc gcacaggcca gactaccgct caggcgctcg gacggcccca
    32641 cgttcagcag ctgcccaatc atcgattttc gtgacatacc taccaacagc ggcaggttga
    32701 aatggtgaaa ttcagccagg cgcgccagta atgaatagtt atgggagaga tttttaccga
    32761 aaccgaatcc ggggtcgagc aacaatttct cttttgcgat acccgcctgc tcgcaacgtg
    32821 ctatttgctc aataaagtag cgattcactt ctgcaaagac atcgtcatac ttcggagctt
    32881 cctgcatggt ttttggattt ccctgcatat gcatcagaca aaccggtaaa ccggtttctg
    32941 ccgccgcctc cagagcgcca ggttcggaaa gggagcggat atcgttaata atgtgagcgc
    33001 caactttcgc tgactcacgg atgacttctg gtttggatgt atctaccgag agccagactt
    33061 cgaagcgttg agcaattgcc tcaaccacag gaataacacg ttgcaactct tcttcaacgc
    33121 taacttccgc cgcccctggg cgcgtggact cgccaccaac gtcaatgatc gtcgcgccag
    33181 cgttgatcat cagattcgca tgtttcaccg catctatcag cgagttatgc gtgccaccat
    33241 ccgaaaagga atcaggcgtg acgttgagga tccccattac gtgaggatgg ctaaggtcca
    33301 gtgaagtgcc ctgggcaaag agtttcatgg tgttatccct ggtatgaatt gataagaaaa
    33361 aaccccggag cacgccccgg ggttttcggt acaaatacag tcatctgatg cgggaactta
    33421 cttgtcgcct aactgctctg acatggtgtt acccgggttc ggcgtacgcg gttcatcaac
    33481 cggacgagga gcctttggac taccattgtc gccagaattg ttagaagcgc ctggttcttc
    33541 ccagcccgct ggcggacgta catcgcgacg tgccatcagg tcatcaatct gcggtgcgtc
    33601 gatagtctca tatttcatga gagcatcttt catcgcatgc agaatatcca tattgtcggt
    33661 cagaagctga cgcgcacgat tatagttacg ctcaatcagt gctttcactt cctggtcgat
    33721 gatacgtgca gtttcatcgg acatatgttt cgctttcgct acgctacggc cgaggaacac
    33781 ttcaccttct tcttccgcgt acagcagtgg acccaatttc tcagagaagc cccactgagt
    33841 caccatatta cgcgccaggt tggtcgcaac tttaatatcg ttggacgcac cggtagaaac
    33901 atgttccggc ccgtagatga tctcttctgc cagacgacca ccgtacagcg tagaaatctg
    33961 gctttccagt ttctgacggc tggcgctgat tgcgtcgccc tcaggcaaga agaaagtcac
    34021 acccagcgca cgaccgcgtg ggataatcgt cactttgtgc accggatcgt gttccggcac
    34081 caagcgaccg ataatcgcat gacccgcttc gtggtaagcc gtcgattctt tctgcgcttc
    34141 cgtcatcacc atggagcgac gttccgcacc catcatgatt ttatctttcg ctttttcgaa
    34201 ctcaatcatc gacacaacgc gtttgttgcc acgagcagcg aacagtgccg cttcgtttac
    34261 caggttcgcc aggtcagcac cggagaaacc aggagtacca cgggcaatga ttgccgcgtc
    34321 gatatcgggt gccaatggta cgcgacgcat gtgaactttc aggatctgct cacgaccgcg
    34381 aacatctggc aagccaacca caacctgacg gtcgaaacgg ccaggacgca gcagggctgg
    34441 gtcaagaacg tccggacggt tagtcgcggc gataacgatg ataccttcgt taccttcgaa
    34501 gccatccatc tcaaccagca tctggttcag agtctgttca cgttcatcgt gaccaccgcc
    34561 cagaccagcg ccacgctggc ggcctacggc gtcgatttca tcgataaaga tgatgcacgg
    34621 tgccgctttc ttcgcctgtt cgaacatgtc acgaacacgg gatgcaccca caccgacgaa
    34681 catttctacg aagtcagaac cggagatagt aaagaacgga actttcgctt cgcctgcaat
    34741 cgctttcgcc agcaacgttt taccggtacc cggaggaccg accatcagga cgcctttcgg
    34801 gatcttaccg ccgagtttct ggaagcggct cggctcgcgc agatactcta ccagttcagc
    34861 aacttcttct tttgcttcgt cgcagcccgc aacgtcagca aaggtcgttt tgatctgatc
    34921 ttccgtcagc atgcgcgctt tgctcttacc aaacgacatg gcacctttgc caccgccgcc
    34981 ctgcatttga cgcatgaaga agatccagac accaatcagc aacagcatcg ggaaccaaga
    35041 gatgaagata gaagccagca ggcttggttc ttcaggcggt tcaccgacaa ccttgacgtt
    35101 cttggtcaac aggttatcca gtaatttcgg atcctgaacc ggaatgtaag tggtataacg
    35161 gttactatct ttcttggtaa cgttgatttc acgtccgttg atacgcgctt cacgaacctg
    35221 gtcgttattg acctcttgta ggaaggtaga gtaatccacc ttacggccat tagactcgct
    35281 gggcccaaag ctctggaata ctgacatcag cacaacggca atgaccagcc agagtattag
    35341 gtttttcgcc atgtcactca agggattaac ctcttattac aactgtgtta aaaacagcgt
    35401 caggatactc tatatccagc atctttcaaa ctttcgtctg aaatctcccg gttagggttt
    35461 acgcccggtc gctacaatat acacttcccg cgaacgtgca cgagaagagt ccggcttacg
    35521 aactttgacc ttcgtaaaca gggagcgaat ttcccttaga tactcatcga aaccttcgcc
    35581 ctggaacacc ttcactacaa aactgccacc tggcgctaat acatcacgac acatttctaa
    35641 cgccagttcc accagataca tggcacgggg gatatccacc gccggtgttc cgctcatgtt
    35701 tggtgccata tcggacatga caacctggac tttgctgtcg ccaacgcgct ccagcagtgc
    35761 tttcatcacc agttcatcac gaaaatcgcc ctgaagaaag tccacaccaa cgataggatc
    35821 cataggtaaa agatcgcaag cgatgatgcg gcctttgccg ccaatttggg tgaccacata
    35881 ttgtgaccaa ccacccggag cagcaccaag gtcgacaacc gtcattcccg gtttaaagag
    35941 tttgtcactt tgctgtattt catcaagttt aaaccaggca cgggaacgta accccttttt
    36001 ctgtgcctgt tgaacatatt tatcgctaaa gtgttcctga agccagcggc tggagctggc
    36061 agaacgcttc ttacctgtca tttaactttc ccatggggat aactcatcgt aaccaattgc
    36121 gtaaattttt acgcgcctat ttggtgatat atgggagatg gcggtagaat gacccgtttt
    36181 caatcccaac gtaagcaaaa aatacgatga atctgagtac taaacaaaaa cagcacctga
    36241 aaggtctggc acatccgctc aagccagttg ttctgcttgg cagtaatggt ttgaccgaag
    36301 gggtgctggc cgagattgaa caagcgttag agcaccatga actcatcaag gtgaaaatcg
    36361 ccaccgaaga tcgcgaaaca aaaaccttga tcgtggaagc tatcgtgcgc gaaaccggcg
    36421 cctgtaatgt acaggtcatc ggtaaaacgc tggtgcttta tcgcccaact aaagaacgta
    36481 aaatctcgct gccacgctaa gattatccta aagttacaca catttgctgt gtaaaacgag
    36541 gggttttccg caggcaggag agcaaaatgc cacgctctgt tcgttgataa aaggccgcat
    36601 agcggccttt ttcctttctt tacaatacat caacatcttg atgtattggg taattcttac
    36661 aggtattcca ccttaattac ttcaaattct acttcgccgc ccggcgtttt gatgaccaca
    36721 acatcatctt cttctttgcc gatcaggcca cgagcaatag gcgagtttac agaaatcagg
    36781 ttttgtttaa agtcagcttc gtcatcgcca acgatgcgat aagtctgttc ttcgtcagaa
    36841 tccagattca gcaccgttac ggtagcacca aaaataacgc gcccattgtt gggcattttg
    36901 gtgacatcaa tcacctgcgc gttcgacagc ttggcttcga tgtctttaat acggccttcg
    36961 cagaaaccct gctgttcacg agctgcgtgg tattcggcgt tttctttcag gtcgccatgc
    37021 tcacgcgctt ccgcgatagc agcaatgatt tcaggacggc gcacagattt cagaaaatcc
    37081 agctcttcgc gtaatttttc agcgccgcgt aaggtcatcg gaatagcttg catttgttat
    37141 acctcttgaa tattcctgat ggggcaagtc ttcacccacc ccggctgttc ggcctgcccg
    37201 gcataactcc tcaccgggac cagaagcaaa aaaataccga cccgggtaca agtcccaggt
    37261 cagctacaat tcacattttg atagtcattt taccctgaag ttcccgaagg gtcatcgttt
    37321 actttatagg gcgttgcgcc gtagtatgac ggctcgattc caggttgtta gcgcgagatt
    37381 atgcgatttt ccagatttat catcggattg accagctgta tagcgttcag tgttcaggcc
    37441 gcgaatgttg atgagtacgt tactcaactc cccgctggtg ccaaccttgc cctgatggtg
    37501 caaaaagtcg gcgcgtcggc ccccgctatt gattaccaca gtcagcagat ggcgctgccc
    37561 gccagtacgc agaaagtgat tactgcgctg gcggcgttga ttcaactcgg ccccgatttt
    37621 cgtttcacca cgacgcttga aaccaaaggc aatgtggaaa acggcgtact taagggtgac
    37681 ttagtggcgc gatttggtgc cgatccgacg ttaaaacgtc aggatattcg caatatgatc
    37741 gcgactttga aaaaatctgg cgtcaaccaa atcgatggca atgtgttgat agatacctcc
    37801 attttcgcca gccacgataa agcccccggc tggccatgga atgacatgac acaatgcttt
    37861 agcgctccgc ctgccgctgc catagttgac cgcaactgtt tctccgtctc gctctacagt
    37921 gccccaaaac ctggtgatat ggcttttata cgcgtggcat cttattaccc cgttacgatg
    37981 ttcagccagg tacgcaccct cccccgaggt tctgccgaag cgcaatactg cgaactggat
    38041 gtcataccag gcgacctgaa ccgctttacg ctgacgggat gcctgccaca acgttctgag
    38101 ccgctcccgt tggcttttgc cgtgcaggat ggagccagct atgccggggc aattctgaaa
    38161 gatgagttaa aacaggcagg tatcacctgg agcggaacac tgctgcgcca gactcaggtt
    38221 aacgaacctg gaacggtagt tgccagtaaa cagtcggccc cgttgcacga tctgcttaag
    38281 attatgctga aaaagtcgga caacatgatc gccgatacgg ttttccgcat gataggccat
    38341 gcgcgcttca atgtgcctgg aacatggcgg gccgggtcgg acgccgtgcg tcagatcctg
    38401 cgccagcaag ccggtgtcga tattggaaac accattattg ccgatggttc agggctttcg
    38461 cggcataacc tgattgcccc cgccaccatg atgcaggtgt tgcaatacat tgcccaacac
    38521 gacaatgaac ttaactttat ctccatgctg ccgctggcgg gctatgatgg ctctttgcag
    38581 taccgtgcag gtctgcatca ggcgggcgtg gatggaaaag tctcagcgaa aaccggttcg
    38641 ttgcaggggg tatataacct ggcgggattc attaccacag cgagcgggca acgaatggcg
    38701 tttgtacaat atctttctgg ctatgcagta gaaccggcgg atcagcgtaa tcgccgtatt
    38761 ccgttagtgc gttttgaaag ccgtttgtat aaagatattt atcagaacaa ttagtcaaaa
    38821 agaaaccccg gcacatggcc ggggttttgc tgattaacgc ttgtaaatga actcaacgcc
    38881 ttcttcgtcg tcttcgtccc agtcatcatc ccagtcctca tcatcctctt cagcaatctc
    38941 ttcaagctgc tggcgatgat aatcatccca catgaattcg actttctctg gctgtttcgc
    39001 ttcttcagcc tgcacgaccg ggttttcaat gataaaggtc atcacatccc agcagagatc
    39061 tttcacgccc agtccactcg ccgcagagat cagataatat ttatcttccc agcccagcgc
    39121 ttcagcaatc gctttcgctt tctcttcagc ttctgcctta tccagcagat cgatcttatt
    39181 gaacactaac caacgcggtt tcgccgccag atcctggctg tatttttcca gctcgctgat
    39241 aataatacgc gcgttttcaa ccggatcggt gccgtcaatc ggatcgatat cgatgaggtg
    39301 caacaggacg cggcaacgtt ccaggtgctt caggaagcgg atacccagac ctgcgccttc
    39361 cgcagcgcct tcaatcagtc ctggaatatc ggcaacaacg aagctctttt cgttgtccat
    39421 tcgtaccaca cccagacttg gcaccagagt ggtaaacgga taatccgcca ctttcggttt
    39481 agccgccgat accgcgcgaa taaaggtcga tttacccgcg tttggcatcc ccaacatacc
    39541 gacgtcagcc agcagcatca gctccagcag cagctcacgc ttatcacccg gcgtaccgtt
    39601 ggttttctgc cgtggtgtac ggttaacgga cgatttgaaa cgggtattgc ccagaccgtg
    39661 ccagccgccc ttagcaacca gcagacgctg accgtgttta gtcatatcgc ccatggtttc
    39721 accagtaccc tggtcgatta cacgcgtacc taccggcact ttaatcgtca cgtctttacc
    39781 gcgcttaccg gtacaatcgc ggcttgcgcc gttctgaccg cgctctgcac ggaaagattt
    39841 ttcaaaacga taatcgataa gcgtgttcag gttttcgtcg gcttccatcc atacgtcacc
    39901 accatcacca ccgtcgccgc catccgggcc gcctttcgga atatactttt cgcggcggaa
    39961 gctcacgcaa ccattaccgc catcacctgc aacgaccaga atcgatgctt catcaacaaa
    40021 cttcatttta ttctccgtaa atcattcgcc tgcgcggggt tgcgatacca ccgttgtatg
    40081 cttacgtaat ccgccccaaa tacgatgacc aatggcggaa tacatcgcgc ccgcaaccac
    40141 gacaaacgca ccgagataac ctaaaaggtt taacatcggt ctggcgaaga aatcgggcca
    40201 ggccagtgat aaaagatctg aaaaaaacag cgtaaacagt ggggtgagcg tgatgatcgc
    40261 gctcacctgc gctgcctgcc agcgagccat cgcttccgcc agggcgccat atcctaccaa
    40321 tgtattcagt ccgcaaaaaa ttaaacatgc gagctgccag tggctaagct gcgctatcac
    40381 tcctggcttt gccagaggga agagcgcaat tgtacataaa gtgtacagca aaaacaggat
    40441 ctgcggtgag gccagccgac gcaataaaac cttttgcgcc acgccataac tcacccaaac
    40501 cgtcgccgca ccgaccccaa agatgactcc ccaggtgtaa tcagtgagct ttgtaaatat
    40561 ctcgaccaga ctggtgttaa aaaacatcac caggccgctc aggagcatca atgcccctac
    40621 aacctgagtg ctgcgcattt tctctttcag gataaatacg ctggcaacca tcatgccaac
    40681 tggcgagagt tgcccaatca cctgcgaagc ggtcggactc aggtattgca aggatgagct
    40741 gaacaggatg aagttcccaa acagcccggc ggtcgccact gccaacaaaa tcaaccagcg
    40801 tggcttacga aacacgcgta atggcggcaa cctcttcttc accgcaagaa tggcacccag
    40861 gccaatactc gccatcaaga aacggtaaaa cacgattgtc ggaggttcca tcacctccag
    40921 cacctgcttc attgcgattg gcaacgcccc ccagcaaatt gctgtggtga gcgccaaaag
    40981 aatgccaatg cctgcctgct gcttcatacc gtattccctg caaagcgcat ttgcggatta
    41041 acgtcgcacc atgctcattt ttaccggtta tcgaatgtaa aaagccccgc aacgtgttgc
    41101 ggggctttca tccgttaccg ggacgcgaaa aacttattca gcttcgatgc tgataaattt
    41161 acggtttttc gggcctttaa cttcgaattt cactttaccg tctgctttag caaacagagt
    41221 gtggtcacga ccgcaaccta cgttagcgcc agcgtggaat ttggtaccac gttgacgaac
    41281 gatgatgcta cccgccagaa cggattcgcc accgaaacgc ttaacgccca ggcgtttagc
    41341 ttctgaatcg cgaccgttac gtgtggagcc gccagccttt ttatgtgcca tttgaaatct
    41401 ctcctcaggt cttaggcgct gatgccagta attttcacat cagtgaacca ctgacgatgg
    41461 ccctgctgct tacgatagtg tttacgacga cgaaacttaa cgattttaac tttctcgcca
    41521 cgaccgtgag caacaacttc agctttgatt acgccgccat caacgaaagg aacgccgatt
    41581 ttgacttctt caccgtttgc gatcatcagc acttcagcga actcaacagt ttcgccagtt
    41641 gcgatgtcca gcttttccag gcgaacggtc tgaccttcgc ttactcggtg ttgtttacca
    41701 ccactttgga aaaccgcgta catataaaac tccgcttccg cgcacacctt ttcaatgatt
    41761 cagagtgcgc tataaatatt cacaataggg cgcgaatatt acgcaaaacg cacgcctttg
    41821 acaagtgcta cagtcaatac acgaagaaaa aaaacacaac ttgtacggta acgtttatct
    41881 gtgccatttt ttcagtacaa tcaccctata ttcctaacca taaaccctaa gttgcctttg
    41941 ttcacagtaa ggtaatcggg gcgaaaagcc cggcttttgc gatgaattta gaaaaaatca
    42001 atgagttaac cgcgcaagat atggcgggtg ttaatgcggc aatccttgag cagcttaatt
    42061 ccgacgtcca actgatcaat cagttaggct attacatcgt cagcggcggc ggtaaacgta
    42121 ttcgtccgat gattgctgta ctggctgcac gagctgttgg ctatgaggga aatgcgcatg
    42181 tcaccattgc tgccctgatc gagtttatcc acacggcgac tctgctacac gacgacgttg
    42241 tggatgaatc agatatgcgc aggggtaaag ctaccgccaa cgctgcattt ggcaatgccg
    42301 ccagcgtgct ggtaggcgat tttatttata cccgcgcgtt ccagatgatg accagcctcg
    42361 gttcactcaa agtgctggaa gtcatgtcag aagctgtaaa cgtcatcgca gaaggtgaag
    42421 ttctgcaact gatgaacgtt aacgatccgg acatcactga agaaaactac atgcgcgtta
    42481 tctatagcaa aaccgcgcgt ctgtttgagg ctgccgcgca gtgttccggg attctggctg
    42541 gctgtacgcc ggaggaggag aaaggcctgc aggattatgg gcgctatctc ggcactgctt
    42601 tccagttgat cgacgattta ctcgattaca atgccgatgg cgaacagtta ggtaaaaacg
    42661 tcggcgacga tctgaacgaa ggtaaaccga cgctgccgct gctgcatgcg atgcatcatg
    42721 gcacaccaga acaggcacag atgatccgta ccgccatcga acagggtaac ggtcgccatc
    42781 ttctggaacc ggttctggaa gcaatgaacg cttgtggatc tcttgaatgg acgcgtcagc
    42841 gcgccgagga agaagcagac aaagccatcg cagcgttaca ggtgctcccg gacacccctt
    42901 ggcgagaagc actcatcggc ctcgcgcaca tcgctgttca acgcgatcgt taatcccctc
    42961 ccttcatccc gcgcagtgcg cgggatgatt ccagtaaatt ccataaaaac actaattcag
    43021 cgctataaca cctgaaaaaa aagggcgatg aatctttata tattctgaat attcacactc
    43081 tttacaggaa ctttttagag caataggcca tcaggagtat agtgatgccc gacagaagaa
    43141 gtgttctgaa tgaaagcgac taacttaagg agtgaggaaa gatggaaagt aatttcattg
    43201 actggcatcc ccctgacatc attgcaggtt tgcgcaagaa aggaacgtca atggcggcgg
    43261 aatctcgcag aaatggtttg agttcctcaa cgctggcaaa cgcattatcg cgcccatggc
    43321 cgaaaggaga gatgattatt gcgaaagccc tgggaactga cccctgggtt atctggccat
    43381 cacgctacca tgatccgcag acccatgagt ttatcgacag aacgcagttg atgcgtagct
    43441 acactaaacc gaaaaaatga gtggtctggc ggtagccccg cgaacggggc tgccagctct
    43501 cagacgatta ctcgcctttc acacgcttaa tatttgcacc taaagcgcgc agtttgtctt
    43561 caatgcgttc gtagccacga tcgatgtgat aaatacgatc aaccaccgtc gtcccttccg
    43621 caatacagcc agccagcacc aggcttgctg atgcacgcag atcggttgcc ataacctgtg
    43681 caccagaaag tttttcaaca ccatgacaaa taacggtatt gctttcgatt tcggcatgcg
    43741 cgcccatacg gctaagctcc ggcacatgca taaagcggtt ttcaaagacc gtttcggtga
    43801 tgaatccggt cccttctgcc accaggttca acagcgtgaa ctgggcctgc atatcggtcg
    43861 ggaatgccgg atgcggcgcg gtacgtacgt taacagcctt cggacgtttg ccatgcatat
    43921 ccaggctaat ccagtcttcg ccgacttcga tgtccgctcc agcgtcacgc agtttcgcca
    43981 gcacggcgtc gagagtatct ggctgcgcgt tacggcagat aattttgccg cgagaaatcg
    44041 ccgccgccac caggaaagta ccggtttcga tacgatccgg tagcacgcga tagacaccgc
    44101 cgcctaaacg ttccacacct tcgatgacga tacgatcggt gccctgaccg ctaattttcg
    44161 cacccagcgt aatcaggaag ttcgcggtat cgacgatttc cggttcacgc gctgcgtttt
    44221 caataatcgt ggtaccttcc gccagggttg cagcacacat gatggtcacc gttgcgccaa
    44281 cgctgacttt atccatcacg atatgtgcgc ctttcagacg accatcgacg gaagctttaa
    44341 cgtaaccttc ttccagtttg atggtcgcgc ctaattgttc gaggccagaa atgtgtagat
    44401 caaccggacg cgcaccgatc gtacaaccgc caggcagtga aacttgcccc tgaccaaagc
    44461 gcgctaccag cggccccagt gcccagatag aagcacgcat ggttttaacc agatcgtaag
    44521 gtgcgcagaa tacattaacg tcgcgggcat caatatgcac agaaccatta cgttctactt
    44581 tcgcacccag ctgacttagc agcttcattg atgtatcgac gtctttcagt ttcgggacgt
    44641 tctggatctc taccggttct tccgccagta gagcggcaaa aaggataggc agagcagcat
    44701 ttttagcgcc ggaaattgtg acttcgccct ggagcttcgt tggcccctga acacgaaatt
    44761 tatccattta gtttgttctc agttaacaat tcatatccgc taccggcgaa tcgcccatag
    44821 ctcaaaagcc gttcagtttg cgatcgcgcg cccactccgc aggggtatac gctttgatcg
    44881 acacagcatg aatgcggtta tccgcaatat attccatcag cgggccatag accgtctgct
    44941 gttttttaac ccgactcatg ccgtcaaaca actcacccac ggcaataacc tgaaagtggc
    45001 tgccatcgcc ggaaacgtgg acttcctgga gggagagagc gttcatcaac acgctctgaa
    45061 tttcattatt ttccatggga tcttcaatca tcagttaata aaccagcgaa acatcttaga
    45121 gcaaagttgc gctggcataa ataagcaaaa agcctcgctg ataaatcaga caaggctcga
    45181 cttgcaggca ggtttgccgg acaggcggtt aacgccatat ccggcctgaa aaaatttaac
    45241 gaggcagaac atcagcaggc aaattataca atttcgccag ggtatacact ttgtcgttta
    45301 ccccctgaag cgtcacattg ttgccctgct ttttcgccag atcgataagc tggagcagca
    45361 gtgccagtcc ccccgtatcc acgcgggaca cacggctaag atcgatgcag gtaatcccct
    45421 tcaccgcttc ctcacgcatt tcccaaagcg gtagcaaaac gtcctgatcc agctctccgg
    45481 ataacgccag cgtgtcaccc gtctgcatcc agctcagtga ctcgctcatt attttttctc
    45541 ttccagagtg attttctgtt gagaaatcga tttcagttgc gcagtcaggc cgtcgatacc
    45601 tttggtacgc agcagcgttc cccactcgtt ttgtttggtg gtgatcatac tgacgccttc
    45661 agcaatcatg tcgtaagcct gccagttgcc cgtctgggag tttttacgcc actggaagtc
    45721 cagacgcacc ggcgggcggc cattcggatc aataatggta acgcgaatcg gcacaatggt
    45781 tttatcgccc agcggctgtt ctggcgcaat ctgataggtt tgaccatgat acatcgccag
    45841 cgcctgaccg taagcctgct tcaggtactc acggaaagcg gcaaagtagg cttcacgttg
    45901 agcaggggtc gcactcttgt aatactggcc cagcaccagc gcaccggcgt atttcacctg
    45961 tacgtatggc agcagttcct gatcaacaat ggtacgcaga taatccgggt tggcccgaat
    46021 ttgcggttgc tcattcttca ggcgatcgaa cgttttctgc gccgcctcgt ccatcagctt
    46081 ataaggattg gtctggtctg ccgcggttgc cgcactcagc ggtgcaatca ccagcaaagc
    46141 gaccatcatt aaacgtttaa acatgcgtcg gttctcctga aattatttcg ttgtacccac
    46201 aggttcagtg gtttcattat tacctggcgc agcagctggc gcatcgccac tattcttatt
    46261 gtcatcgcct ttactaccgt aaaggaactg accaatgaga tcttccagca ccatcgcaga
    46321 cttagtgtcc tgaattgtat cgccatcctt caggatagca gtccccagtt ccgggtcttc
    46381 aaaaccgacg tttaatgcca gatattgttc ccccagtagg ccggaagtac gaatgctcag
    46441 cgaactggta tctggaatgt ggttataacg ttgttcaatt tccagcgtta cgcgtggcag
    46501 ataggttttc gggtccagcg taatatccgc cacccggccc acaacaacgc caccaatact
    46561 gaccggagag cgcgctttca ggccgccaat gttatcgaac gtcgcataaa gcgtgtaggt
    46621 cggttcagta cgtatggacg tcacgttcgc cgccttcagg caaacaaaca gagccgccag
    46681 cagtgctgct aataaaaaga tacccaccca aatttcattt tttttcgttt gcatgtactc
    46741 aattcccaaa catcaatgcg gtcagcacaa aatccagccc cagaacagcc agagacgagt
    46801 ggacaacggt gcgagtggtt gcccggctaa tcccggcaga cgtcgggatg gcgtcgtagc
    46861 cgttaaacaa cgaaatccac gtcaccgtga tggcgaacac cacgctctta atcagacagt
    46921 tgaccagatc catacgccag tcgacggcat tttgcattgc cgaccagaag aacccgctat
    46981 caatgccttt ccaactgacg ccgaccagag atccgcccca gatccccacg gcgacgaaaa
    47041 taaccgtcaa cagtggtaat gaaataaccc cagcccagaa acggggagaa ataacccgac
    47101 gcagcggatc caccgccatc atctccatac tggagagttg ctctgtagcg cgcatcaggc
    47161 cgatttctgc ggttagcgcc gaaccagcac gcccggcaaa caacaacgcg gcaaccaccg
    47221 gccccagttc acgcagtagc gataacgcca ccagcatacc cagactggtt tccgcactat
    47281 aagtggtcag aaccagataa ccttgcagcc ccaacaccat tccgatgaac acgccagaaa
    47341 ccacaataat cagcatcgac aggacgccga cattatagag ctggcgcacc agcagcggcg
    47401 catgtttgcg aaattccggt ttgccgacca gcgcattgaa taacattaac ccggcccgcc
    47461 cgaacgttct cagggtttta atccctttat gtccgagcga cgccagcgca tttaacagca
    47521 tgagtggctt aactccctgg taaaagatca gcgtgataat cgccggcagg atagcggaac
    47581 ggaacaggcc cgtcagctat cccgtccaga aactgacgta cgcgcggatc aggattcgcc
    47641 tgcaacgcct gggcactgcc atgagcgaca atttttttgt ccgccaggat ccaggcgtga
    47701 tccgcaatac ttaacacttc cggcacatcg tgagaaacca ccacacaagt cacgcccagc
    47761 gcgctgttca gctcagaaat cagcttcacc agtacgccca tggtgatggg atcttgccca
    47821 acaaaaggtt catcaaacat gatgagatcc ggctccagcg caatcgcacg cgccagcgct
    47881 gcacgccgcg ccatcccacc ggaaagttca gaaggcatta gtttagccgc tccacgcagc
    47941 cccacggcct ccagcttcat catcaccgta ctatgcaaca atggcgcggg aagttgggta
    48001 tgttcgcgca gtggataggc gacgttgtca aatacgttca tatcagtgaa caacgccccg
    48061 gactgaaata acatgctcat ccgtttgcgc actgtataca ggcgcgaacg agacatcgcc
    48121 ggaatattct caccatcgaa aaggatctca ccatgatctg gtgcgatttg cccgccaatc
    48181 agacggagta gcgtcgtttt accgatgccc gatggcccca tgatcgccgt gatcttccct
    48241 cgcggcacgg tcagggaaat attatcgaag atgcagcgat tgccacgcgt aaaactgaca
    48301 tcgcgcatat cgactaaatt cgccacagac tgctccataa ttcacccttc gtcttgcgtt
    48361 gattttctaa gcatggcgct caatttaacc ttgaacccaa catatttaca gaatattacc
    48421 cgccgtggtt agcgaaagct ggcatttgtt ttacttttta gccgcataaa gtcaaaatta
    48481 agaatccgtt acggctttct gaaaatcttc agcggaccgg cgagtatacc tgaagaaagg
    48541 acgttagatg cttttagcta cggcactgtt aattgttggt ttacttttgg tcgtttacag
    48601 tgccgaccgc ctggtttttg ccgcgtctat tctttgccga acctttggca tcccgccgct
    48661 gatcatcggc atgacggtgg tcagtattgg tacatcgtta ccagaaatca tcgtctcgct
    48721 tgccgcgtct ctgcacgaac aacgcgattt agccgttggt acagccctcg gctcaaacat
    48781 tatcaatatt ttgctgatcc tcggtctggc tgcgctggtt cgtcctttta ccgtccattc
    48841 cgatgttctg cgtcgtgaat tacccttaat gttgttggtc agcgtagtgg ccggttccgt
    48901 actctatgac ggacaactta gtcgcagcga tggtatcttt ctcctctttc tggctgtgct
    48961 atggctgctg ttcattgtta aacttgcacg tcaggctgaa cgtcagggga ctgacagcct
    49021 gaccagagag cagcttgcag agctgccgcg tgacggcgga ttgcccgtcg cgtttttatg
    49081 gctcggcatt gcgcttatca tcatgccagt ggccacgcgg atggtggttg ataacgccac
    49141 ggtgctggcg aattactttg ccatcagcga gttgacgatg ggtctgacgg caattgctat
    49201 cggaaccagc ctgccggaac tggcaaccgc aatagcgggg gttcgcaaag gtgaaaacga
    49261 cattgctgtc ggaaatatca ttggcgcaaa catttttaat attgtcatcg tgttgggttt
    49321 acccgcgctg ataacaccag gagagattga tccattggcg tacagtcgtg actacagcgt
    49381 gatgttgctg gtgagcatta tttttgcgtt gctgtgctgg cggcgctccc cgcaaccggg
    49441 ccgtggtgta ggggtattat taactggcgg atttatcgta tggctggcga tgttgtactg
    49501 gttatcgcca atactcgttg aataactgga aacgcattat gtcgcacgta gagttacaac
    49561 cgggttttga ctttcagcaa gcaggtaaag aagtcctggc gattgaacgt gaatgcctgg
    49621 cggagcttga tcaatacatc aatcagaatt tcacgcttgc ctgtgaaaag atgttctggt
    49681 gtaaagggaa agttgtcgtc atggggatgg ggaaatcggg gcacatcggg cgcaaaatgg
    49741 ccgcaacgtt tgccagcacc ggtacacctt catttttcgt ccatcctggt gaagccgcgc
    49801 atggtgattt aggcatggtc accccacagg atgtggtgat tgctatctct aactcaggtg
    49861 aatccagcga aatcacggcc ttaattccag tgcttaagcg tcttcacgta ccgttaatct
    49921 gcatcaccgg tcgcccggag agcagcatgg cgcgcgccgc agatgtgcat ctgtgtgtta
    49981 aagtagcgaa agaagcctgt ccgttagggc tggcaccgac cagcagcacc accgccacgc
    50041 tggttatggg cgatgccctc gctgtcgcgc tgttaaaagc acgcggcttt actgctgaag
    50101 attttgcgct ctcacaccca ggcggcgcac tgggtcgtaa acttctgctg cgcgtaaacg
    50161 atattatgca tacgggcgat gagatcccgc atgttaagaa aacggccagt ctgcgtgacg
    50221 cattgctgga agttacccgc aaaaatcttg gtatgactgt catttgcgat gacaatatga
    50281 tgattgaagg catctttacc gacggtgatt tacgccgtgt cttcgatatg ggagtggatg
    50341 ttcgtcagtt aagtattgcc gatgtgatga cgccgggggg aatacgcgtg cgccctggca
    50401 ttctggccgt tgaggcactg aacttaatgc agtcccgcca tatcacctcc gtgatggttg
    50461 ccgatggcga ccatttactc ggtgtgttac atatgcatga tttactgcgt gcaggcgtag
    50521 tgtaaagatt caaggataaa caacaatgag caaagcaggt gcgtcgcttg cgacctgtta
    50581 cggccctgtc agcgccgacg ttatagcaaa agcagagaac attcgtctgc tgatcctcga
    50641 cgttgatggc gtactgtcag atggcctgat ttatatgggc aataacggcg aagagctgaa
    50701 agcgttcaat gttcgtgacg gttatggcat tcgttgtgcg ctcacctctg atattgaagt
    50761 cgctatcatt accgggcgaa aggctaaact ggtagaagat cgttgtgcca cattggggat
    50821 cactcacttg tatcaggggc agtcaaacaa actgatcgcc tttagcgaac tgctggaaaa
    50881 actggcgatt gccccggaaa atgtggctta tgtcggcgat gatctcatcg actggccggt
    50941 aatggaaaaa gtgggtttaa gcgtcgccgt ggccgatgct catccactgt tgatcccgcg
    51001 cgccgattat gtgacgcgca ttgctggcgg tcgtggcgca gtgcgagaag tttgcgactt
    51061 attactcctg gcgcagggca aactggatga agccaaaggg caatcgatat gagtaaagcc
    51121 agacgttggg ttaccattgt gctatcactg gcggttctgg tgatgatcgg cattaatatg
    51181 gccgaaaaag acgataccgc ccaggtggtc gtcaacaaca atgatcccac ctataaaagc
    51241 gagcatacgg acacgctcgt ctataaccca gaaggggcac taagctatcg attgattgct
    51301 caacacgttg aatattattc cgatcaggcc gtttcgtggt ttacgcagcc ggtacttacc
    51361 acgtttgata aggataaaat cccgacatgg tccgtaaaag cagataaagc caagctgacc
    51421 aatgaccgga tgctctattt atatggacac gttgaagtca acgcacttgt gccggactct
    51481 caacttcgca gaatcacgac ggataacgcg cagatcaatc tggtgacgca ggatgttacc
    51541 tctgaagacc tcgtcacgtt atacggaaca acatttaact ccagcggtct gaaaatgcgc
    51601 ggcaacttac gcagcaagaa cgccgagctg attgaaaagg ttagaacatc ctatgaaatt
    51661 caaaacaaac aaactcagcc ttaatcttgt gcttgccagc tcacttctgg ccgccagcat
    51721 tccggcattt gccgtaaccg gagacactga tcagccgatc cacattgaat cggaccagca
    51781 atctcttgat atgcaaggca acgtggttac ctttaccggt aatgtcatcg tcacccaggg
    51841 caccatcaaa attaatgccg acaaagtggt cgttacccgt ccgggcggcg aacaaggtaa
    51901 agaagtgatt gacggctacg gtaaaccggc aacgttctac cagatgcagg acaacggtaa
    51961 acccgttgaa ggtcatgctt cccagatgca ctacgaactg gcaaaagatt ttgtcgttct
    52021 gacgggtaat gcttatctgc agcaggtcga tagcaacatt aagggcgata agatcactta
    52081 cctggtgaaa gagcagaaaa tgcaggcttt cagcgacaaa ggcaagcgcg taacaaccgt
    52141 tctggtgccg tcgcagctgc aggacaaaaa caacaaaggc cagaccccgg cacagaagaa
    52201 gggtaattaa ttcgttatgg caacattaac tgcaaagaac cttgcaaaag cctataaagg
    52261 ccgtcgcgtg gtagaagacg tcagcctgac cgtcaactcc ggggaaattg tcggtctgct
    52321 ggggccaaac ggtgccggta agaccaccac tttctatatg gttgttggca ttgtgccgcg
    52381 cgatgcgggc aacatcatta ttgatgatga cgatatcagt ctgctgcctc tgcatgcacg
    52441 cgcgcgccgc ggtatcggct atctgccaca ggaagcctcc attttccgtc gcctcagcgt
    52501 ttacgataac ctgatggcgg tactgcaaat tcgtgacgac ttgtctgctg aacaacgtga
    52561 agaccgcgcg aacgagctga tggaagagtt tcacattgag cacctgcgtg acagcatggg
    52621 gcagtcactc tccgggggtg aacgtcgccg tgtagaaatt gcccgcgcac tggctgcgaa
    52681 tccgaaattt attctactcg acgaaccgtt tgccggggtt gacccgatct cggttatcga
    52741 cattaaacgc atcattgaac acctgcgcga cagcggcctg ggcgtactga tcactgacca
    52801 caacgtgcgt gaaacactgg cggtttgtga acgcgcttat atcgtcagtc aggggcattt
    52861 gattgcccac ggcacgccta cagaaatctt acaagacgaa cacgttaagc gtgtatacct
    52921 tggggaagac ttcagactct gatagggtag aagtttgcga cgttttagca ggagagtacg
    52981 attctgaaca tgaagcaagg tttgcaactc aggcttagcc aacaactggc gatgacgcca
    53041 cagcttcaac aggcgattcg tctgttgcag ttgtcgacgc tggaacttca gcaggagcta
    53101 cagcaggcgc tggagagtaa tccgctgctt gagcaaatcg acactcatga agaaatcgac
    53161 accagcgaaa cgcaagacag tgaaacgctg gacaccgccg acgcgctcga acaaaaagag
    53221 atgccggaag agctgccgct cgatgccagt tgggacacca tttacaccgc tggtacacca
    53281 tccggcacca gcggtgacta cattgacgat gagctgccgg tctatcaggg cgaaacgacg
    53341 cagaccttgc aggattacct gatgtggcag gtcgagctga caccgttttc cgacactgac
    53401 cgcgctattg ctacctctat cgtcgatgcc gttgatgaca ccggttatct gactgtcccg
    53461 ctggaagata ttctcgaaag tatgggcgat gaagagatcg acatcgacga ggttgaagcc
    53521 gtccttaagc ggatccaacg gtttgatccg gtcggtgtag cggcaaaaga tctgcgtgac
    53581 tgcctgctaa tccaactctc ccaattcgat aaaaccacgc cgtggctgga agaggccaga
    53641 ctgatcatta gcgatcatct cgatctgtta gccaatcacg acttccgcac tttaatgcgc
    53701 gtcacgcgtc tgaaagaaga tgtgctgaaa gaagccgtca atctgatcca gtcgctcgat
    53761 ccgcgccccg ggcagtcgat ccagactggc gaacctgagt atgtcattcc agatgtgctg
    53821 gtgcgtaagc ataacggtca ctggacggta gaactcaaca gtgacagcat tccgcgtctg
    53881 caaatcaacc agcactacgc ctcgatgtgc aataacgcgc gcaacgatgg tgacagccag
    53941 tttatccgca gcaatctgca ggatgccaaa tggttgatca agagtctgga aagccgtaac
    54001 gatacgctac tgcgcgtgag tcgctgtatc gttgaacagc agcaagcctt ctttgagcaa
    54061 ggtgaagaat atatgaaacc gatggtactg gccgatatcg cccaggctgt cgaaatgcat
    54121 gaatcgacga tatctcgcgt gaccacgcaa aaatacctgc atagtccacg aggcattttt
    54181 gaactgaagt atttcttttc cagtcacgtc aataccgagg gcggcggcga agcttcctcc
    54241 acggcgattc gtgcgctggt gaagaaatta atcgcggcgg aaaacccagc gaaaccgttg
    54301 agcgacagca agttaacctc tttgctgtcg gaacaaggta tcatggtggc acgccgcact
    54361 gttgcgaagt accgagagtc tttatccatt ccgccgtcaa accagcgtaa acagctcgtt
    54421 tgacccaacc gataaggaag acactatgca gctcaacatt accggaaata acgtcgagat
    54481 caccgaggca ctgcgcgaat ttgttagagc caaatttgcc aaacttgagc aatattttga
    54541 ccgaatcaac caggtctatg ttgttctgaa agtggagaaa gtcacccaca cctcagatgc
    54601 aacactgcat gtaaacggtg gtgaaattca tgccagcgcg gaaggtcagg acatgtacgc
    54661 tgccattgat ggcttaattg ataagctggc acgccagttg accaaacata aagataaact
    54721 gaaacaacac taattgtccg ggcaattagc atgtgcatgg cggcctgttg tgcggcacaa
    54781 cgggccattt gtacggttaa tgctccgagc ctgttccact gtttgagtgg gcaggttctt
    54841 aggtgaaatt atgacaaata atgatacaac tctacagctt agcagtgttc ttaacaggga
    54901 atgtacgcga agccgcgttc actgtcagag caaaaaacgc gccctggaaa tcatcagcga
    54961 gctggcggcg aaacaactta gcctgccgcc tcaggtggtt tttgaagcta tcctgacgcg
    55021 ggaaaaaatg ggcagtaccg gtatcggcaa tggtattgcc attccgcatg gcaaactgga
    55081 agaagatact ctgcgcgccg ttggcgtttt cgttcagctc gaaacgccta tagctttcga
    55141 tgccatcgac aaccaaccgg tggacttact ttttgccctg ctggtgccgg cagaccaaac
    55201 taaaacgcac ctgcatactc tgtcgctggt ggcgaaacgt ctggcggaca aaaccatctg
    55261 ccgccgttta cgcgcagccc agagcgatga agagctgtat caaatcatta cggataccga
    55321 aggtactccg gatgaagcgt agttattcgg taatgtctct tttagacgtt gtgaggagaa
    55381 acagtacatg gtactgatga tcgtcagcgg acgttcaggt tcaggtaaat ctgtcgccct
    55441 gcgtgcgctg gaagatatgg gtttttactg cgtggataac cttcccgtag tgttgttacc
    55501 cgatctggct cgaaccctgg ccgatcgcga gatttctgcc gccgtcagca ttgatgttcg
    55561 taatatgccg gagtcaccag aaatattcga acaggcgatg agtaacctgc ctgacgcttt
    55621 ctcaccgcaa ctcctgttcc tggatgccga ccgtaatacc ttaattcgtc gttacagtga
    55681 cacgcgccga ctgcatccgc tttccagcaa aaacctgtcg ctggaaagtg ctatcgacaa
    55741 agaaagcgat ttgctggagc ctctgcgttc gcgagcggat ctgattgtcg atacctcaga
    55801 aatgtccgtt cacgagctgg ctgaaatgct gcgtacccgt ctgctgggta aacgtgaacg
    55861 cgaactgacc atggtctttg agtctttcgg cttcaaacac ggtatcccta tcgatgcaga
    55921 ttacgtcttt gacgtgcgct tcttgccgaa cccgcactgg gatccgaaac tgcgtccaat
    55981 gacaggtctt gataaacctg tcgccgcgtt cctcgaccgc cacacagaag tacacaattt
    56041 tatctaccag acgcgaagct atcttgagct atggttacct atgctggaaa ccaacaaccg
    56101 tagctacttg acggtcgcca ttggttgtac cggcgggaag caccgttcgg tgtatattgc
    56161 agagcaactg gcagactact tccgctcgcg cggtaaaaac gtccagtcac gccatcgtac
    56221 gctggaaaaa cgtaaaccat gaccgtcaag caaactgttg aaatcacaaa caagctgggc
    56281 atgcatgccc ggcctgcaat gaagctgttt gaattaatgc agggttttga cgctgaagtg
    56341 ctgttacgta atgacgaagg caccgaggcc gaagccaaca gcgttattgc gctgctgatg
    56401 ttggattctg ccaaaggacg gcagattgaa gttgaagcga ccggcccaca ggaagaggaa
    56461 gcactggccg ccgttatcgc cctctttaac tctggttttg atgaagatta atcttcatca
    56521 ctttgacata caactccctt caaactcccc ccgacaataa gaaaatcacg tactgaaatc
    56581 gttctcaatc aacgtcattt gtacattttg tgcgcttttc actttcagaa gaaccttaag
    56641 aaaaccttaa gaggcattgt ttaggttttg tttaagttaa tcgaccatac tggagatcgt
    56701 cagaaaatat ttccaggaga tggcatgatt cgcttatctg aacaaagtcc cctgggcacc
    56761 ggaagacatc ggaaatgtta tgcgcatccg gaagatgccc aacgctgtat caagattgtc
    56821 taccatcgtg gcgatggtgg cgataaagag atccgccgtg agttaaagta ctacgcgtat
    56881 cttggtcgcc ggttaaaaga ctggagtgga ataccgcgct atcacggtac cgttgagacg
    56941 gattgcggaa ccggttatgt ctacgatgtg attgcagatt ttgacggcaa accctccatt
    57001 acgctgaccg aatttgctga acaatgccgt tacgaagaag acattgcaca actgcgccag
    57061 ctactgaaac agctgaagcg ttatttgcag gataaccgta tcgtgacgat gtcgctgaag
    57121 ccgcagaata tcctctgtca ccacatcagt gagtcagagg ttatcccggt cgtctgcgac
    57181 aacattggtg aaagcacatt gattccactg gcgacctggt cgaagtggtg ttgtctgcgt
    57241 aagcaagagc ggttatggaa acgatttatc gcccaaccgg cgctggctat tgccctgcag
    57301 aaagatctgc aacctcgtga gagcaaaacg ctggccctca cttcgcgcga agcttaatcc
    57361 agctggtgtt gttgcataaa cggctcaccg cctaactgat acatctgccg taaaatccac
    57421 gcctgacggc tacgcacgta gcccgatggc gaggagactt tgaaacgaag tggattaggt
    57481 aatacagctg ccagtaatgc agcttccgac cgggtaagtt tgctcgcggg tttgtggaaa
    57541 taacgttgtg ccgcagcttc gacgccaaac acaccgtcgc caaattcggc gatattcagg
    57601 taaaccgtca ggatacgctt tttgctccag actgtttcta tccccagcgt taatccagct
    57661 tccagccctt ttcgtaccca gctacgccca tcccataaaa agagattttt ggccgtctgc
    57721 tgagaaatcg ttgaagcacc gcgaatacgg ttttcattgc gctcgttgtg cgccagggct
    57781 ttctcaatgg aagcgacatc aaagccccgg tgctcaggaa atttctgatc ttctgcggca
    57841 atcaccgcca gtcccatcca cggcgagatt tgatccatac tgacccagtc agaatgtgcc
    57901 acgtaacgaa aattgccatg cagccaggcg ctgacctgtc gctcgaccat taccgctgag
    57961 aagggaacag gcgcaacact aaacaacgcg atgcccccgc cccagaaaac ggcgaggaca
    58021 accattaacc gcaaaagaaa acggcgaacg aaactaaaca ccgttaagcg gcttttactc
    58081 attcagccag aaccagcacg cgggaaacca gcttatcaat gccgctcgcc gcttctgcaa
    58141 tgttctgcgc cagcatatat gctggggtgg tgacaatctt attgtcttca tcaaccacga
    58201 tatcatcgac aggacagggc acatgctccg cgcccatctc ttccagcact tctgcggtat
    58261 cgatatcagt accgatggtc aaacgcagcg ggaaatcgaa aattttcggc agcatcgccg
    58321 gggcaataca cataaaacca agcggttttc cggcctgatg catcgcttgt gccagcgcct
    58381 ttaattcacg gtcaacggtg cattcgctac caagagtggc aaaattgctt aaattcttcg
    58441 ccgcgccaaa cccccccggc acaatcaacg catccagttc agcggcatcg gcctgggcca
    58501 gaggacggat ttcaccgcgc gttattcgtg ccgcttcaat cagcacgttg cgcgtttccg
    58561 tcatcgcttc gccagttaaa tggttgataa catcaacctg ctgcttatca ggtgcaaagc
    58621 agaccgcctg cgcaccgctg cgtgaaatag ctaacaacgt caacaccgct tcatgaattt
    58681 cagaaccgtc atagacgccg catccgctca gaattacgcc aattttcttc attgtgatca
    58741 tcctttcgca attgactgaa acacattaat ttttttaata aaaatggtac gcatcacaca
    58801 tttaactgat tcatgtaaca aatcatttaa gttttgctat cttaactgcg tgcggcctga
    58861 aaaacagtgc tgtgcccttg taactcatca taataattta cggcgcagcc aagatttccc
    58921 tggtgttggc gcagtattcg cgcaccccgg tctagccggg gtcatttttt agtggctttt
    58981 gccacccacg ctttcagcac ttctacgtcg tgacgccact cttctttcat ctcttcaatc
    59041 cattcaccga cgttatcttc ccaggcagga aggtcaggag actgaatttg ctgacccagc
    59101 tgttgcagat ggcgtaaccc cactgacccc gccgcacctt taattttatg tccttcctca
    59161 acaatgcctt ttttatcctg cgccgtcaga ttcgactcca gcacgctgac atagcccggc
    59221 atcatcttct caaacaccgc taacccgtcg gtgatcagct tcggtcctac aagttcgaga
    59281 tactgttcca gcatgggaat atcgagcaat gcttctgatt tactgttctc ttctgtcgtc
    59341 accgtactct cctcatcatc ctgggtatcc cagaatttct tgatcatcgc ggttagcgcc
    59401 ggaaccgaaa gcggcttact cagcacatca tccattccag cattgaggta ctcttgtttg
    59461 tctttcagca cgttagcggt taaggccacc agcggcggta aatcctcgcg cggataacgc
    59521 ttcgtcagtt cacgagagat atccagcccg gtcatatctg gcaactgaat atccagcaac
    59581 accaggtcgt attcgcccgg tttaaacatc tccagcgccg ccttgccggt catggcgaca
    59641 tcaacgctgt tacctaattt ttccagcaca gaacgcgcaa caatcacgtt cagttcaatg
    59701 tcttccacca gcagcacatt cagcgccggt aaaggcatat cgtcttcatc aaacgcatca
    59761 tcgacctctt ctgctaccga cggtgcgtgg atcgtcaacg taaaggttga acctttgccc
    59821 tgttcgctgg taaccgtaat atcgccgccc atatttttcg ccagacgacg agaaacggcc
    59881 agaccaatac cggtgccggt ggcaggttta ccgccatgac tgtctttcac ctggtaatac
    59941 atggcgaaaa ttttatccag ctcatcctgc ggaatgccga taccagagtc ttccacttca
    60001 aaatgcagca tatcgccttc atcgtagcgc acgcgcacgg taacctggcc ttgctgggtg
    60061 aatttgacgg cgttactgat gaggttccac aggatctgcc gtaaacgcgt cccgtcggta
    60121 atgacctgat gcggtaatgg cagcgtcggc tccaggttaa agcgcaatcc tttttgttgc
    60181 gcctgcaatg cggagagatt ttccagatcg gcaaggaagc tggtgaaatc aaccggttga
    60241 ttatcaagct ggaccttgcg ccgttccatc ttatccatgt cgataatatc gttaaagata
    60301 ttccccagcg tgacggccga aacatggatg gtcttgagat atttttcctg ctcggcggtg
    60361 agttcggtat ccagcagaat gcggctcaga ccgacgatac cgttcagcgg tgtacgcaat
    60421 tcgtgactga tggtggagat aaacgtcgtt ttgtcgcggc tggcccgttc aagcgcatcc
    60481 tgataccgct tacgctcggt aatgtcgcga ccaaagccca tcaaaccgtg acgtttaccc
    60541 acgcggtcgt agtacggcac tttacggatt tcaaagcagg ctttgcgccc gtccgggtaa
    60601 tccagccact gttcataggt cagtgacaca ttatgacgga acactttttc atcggtttca
    60661 atgacttttg cggcggcttc cggtgagtaa acatcagcag gtttcaggtg aaccagttgt
    60721 ttttcgcttt ttccggtcag cagctccatc gcgcggttac agccggaaaa ctctttatct
    60781 tcgttacgat aaaaaaccag gtcgggtgaa gcatcaagga aggaacgtaa gaatgaggat
    60841 tgctgctcga gctgaatttg tgtctcttcg cgctctttga tttcaatttt cagttggccg
    60901 aaggtttcct gcagttctgc ttccgctttt tcacgaacgg caatttcctg atttagctgg
    60961 gcaatattat cttttaactg aacgttgagg ctcaaatcgc gctcgcgcat ctcctccagt
    61021 ttttgcacca gccgtgacag acgttgtcgt gactcctcca gttgctcgac gaccaccgat
    61081 agaaagtaga ccgcccacgg cgtaatcagc aaaccaaaga agatagaacg aataacatca
    61141 atgctttcga cctgaccatg cagcaccatg gttaccgcca tttgtaccac aatggcaaga
    61201 acgacgaggg ccagcgccag caacattgag aagcgcacca gacctaactt catcatcagg
    61261 tcaacataat actgcgccag cagacgaatt tgcttcatta gggaattcct tcacgacaac
    61321 ctgccccaat aatacccaat tctgacagtt acgttataaa ttgtgtgaga aatgcggaat
    61381 tcccctcaac gacctactcc gttggaggta gccacggacg tcccagcgcc gatccctgta
    61441 cgccatgttc attcagatag cgatccagct cgaccattcc cgtccatcga ttttcgcacc
    61501 acagcggagc aagcagcgtc gggcgacggg cgctggcgga aatgcggtgg tagatcactt
    61561 ccggtggcgt atggcgaatc atttctccgg cagtgagcgt gtaatcctcc agttcaatac
    61621 cgttcaaacg ccccgcttcc caggctttcg ccataatgct gcctttcaca atatgcagcg
    61681 gatgcagctt tatgccgtcc acgccggttt caaccactcg ctccagcgtt tgcaaacatt
    61741 cagcctgccc ttcaccaggc aggccgacaa ttaagtggga acacaccttc agcccgcgct
    61801 gacgtgccag ctgggttgta cgctgataac aggcaaaatc atggccgcgg ttgatgcgat
    61861 gcagtgtttt gtcgtgggcg gtttgtagcc ccagctccag ccacacttcg taaccctggt
    61921 ccttatattc gcaaagcaga tccagcaccg catccggcac gcagtccggg cgggtaccaa
    61981 cacacaaacc gacaatattg gcctggctca ccgcctgctg atacatagaa cgcagcacct
    62041 gaacttccgc aaaggtgcta gtatacgcct gaaagtaggc cagatagcgt ttagcgcggt
    62101 taactaaatt cgcctggtgc gccagttgct cggcaatgga acgatgctgc tgcgcttcat
    62161 cggcaaacga ggcaacatta cagaatgtgc agccaccacg cccgatggta ccgtcacggt
    62221 tagggcagct aaaaccgccg tgcagcgtca gcttatgcac cttttgccca taacgacggg
    62281 tgagatcacc accaaacata ttgactaatt tctgtaactg cataatctga tagaccgcgc
    62341 cttgaaaaga ggccaaagcc tgccattttt agctcaattc ggcgatgacc tggatcaatc
    62401 gtcccggcct gcttttatca actgcataat caatcaaaat taccgaaatt tcatgcataa
    62461 tcacataaat cacttttgct tatcttgtgt cagatttttt tatctcctta tggattttag
    62521 gcaaaaacag tagcatgaaa cgtcattacc aattaaggca gtataaaatg ctggttttct
    62581 cgtcagttca aggtaggata agggttaaca cgcctttatg acagtcagga attgactgtt
    62641 tctctaacga cttccctttt agccttaaag ataaaattca ttttaatttc agttacttaa
    62701 taaagaattt tgcgctaaag cacatttctg taccaataag cttgccattt gacctgtatc
    62761 agctttcccg ataagttgga aatccgctgg aagctttctg gatgagcagc ctgctcatca
    62821 tatttatgca gtaattgaga tcccctcttc accgtattaa ccgatgcgaa aaggacaaca
    62881 agggggcgaa tacgaggcgc gcgtatgaca cgcaaccccc gtcgccacgc tctttctgtg
    62941 cccgtgcgca gcggttcgga agtggggttc ccgcagagcc tgggggaggt tcacgatatg
    63001 ttgtacgata aatcccttga gagggataac tgtggtttcg gcctgatcgc ccacatagaa
    63061 ggcgaaccta gccacaaggt agtgcgtact gcaatacacg cactggcccg catgcagcac
    63121 cgtggcgcga ttctcgccga tggtaaaacc ggtgacggtt gcggcttgct gttacaaaaa
    63181 ccggatcgct tttttcgcat cgttgcgcag gagcgcggct ggcgtttagc aaaaaactac
    63241 gctgtcggga tgctcttcct gaataaagat cctgaactcg cggctgccgc acgccgcatc
    63301 gttgaagaag agctgcaacg cgaaaccttg tcgattgtgg gctggcgtga tgtccccact
    63361 aacgaaggcg tgctgggtga aatcgccctc tcctctctgc cccgcattga gcaaattttc
    63421 gttaacgctc cggcaggctg gcgtccgcgg gatatggagc gccgtctgtt tatcgcccgc
    63481 cgccgcattg aaaagcgtct cgaagccgac aaagacttct acgtctgtag cctgtcgaat
    63541 ctggtgaaca tctataaagg tctgtgtatg ccggcggatc tgccgcgctt ttatctggat
    63601 cttgcggacc tgcgtctgga atcggccatc tgcctgttcc accagcgctt ctccactaac
    63661 accgtaccgc gctggccgct ggcgcagccg ttccgctacc tggcgcataa cggtgaaatc
    63721 aacaccatca ccggtaaccg ccaatgggcg cgtgcgcgta cctataaatt ccagacgccg
    63781 cttatccctg atctgcacga cgccgcaccg ttcgtcaacg aaaccggctc tgactccagt
    63841 tcgatggata acatgctgga actgctgctg gcaggcggga tggatatcat ccgcgccatg
    63901 cgtctgttag tgccacccgc ctggcagaac aacccggata tggacccgga actgcgtgcc
    63961 ttctttgact ttaactccat gcatatggag ccgtgggatg gcccggcggg catcgtgatg
    64021 tccgacggtc gttttgccgc ctgtaacctc gaccgtaacg gtctgcgtcc ggcgcgctac
    64081 gtcatcacca aagataagct catcacctgc gcctctgaag tcggtatctg ggattaccag
    64141 cctgacgaag tggtcgaaaa aggccgcgtc gggccaggcg aactgatggt tatcgacacc
    64201 cgcagtgggc gtattctgca ctcggcagaa accgatgacg atctgaaaag ccgccatcca
    64261 tataaagagt ggatggagaa aaacgtccgc cgactggtac cgtttgaaga tctgcccgat
    64321 gaagaagtgg gtagccgcga attggacgac gacacgcttg ccagctacca gaaacagttt
    64381 aactacagtg cggaagagct ggactccgta attcgcgtac tgggcgaaaa cggtcaggaa
    64441 gcggtcggtt cgatgggcga tgatacccca ttcgccgtgc tctccagtca gccgcgcatt
    64501 atttacgact acttccgcca gcagtttgcc caggtgacta acccgccaat cgacccgctg
    64561 cgtgaagcgc atgttatgtc gctcgccacc agtatcggtc gtgaaatgaa cgtcttttgc
    64621 gaagcagagg gccaggcgca ccgtttaagc tttaaatcgc cgattctgct ctactccgat
    64681 ttcaaacagc tcacgacgat gaaagaggag cactaccgcg cagatacgct ggatatcacc
    64741 tttgacgtca ctaaaaccac gctcgaagcg acagtcaaag agctgtgcga caaagccgaa
    64801 aaaatggtac gtagcggcac cgtgctgctg gtgctctccg accggaatat cgctaaagat
    64861 cgcctgccgg ttccagcccc gatggcggtt ggcgcgatcc agacccgtct ggtcgatcaa
    64921 agcctgcgtt gcgatgccaa catcatcgtc gaaaccgcca gcgcccgcga tccgcaccac
    64981 ttcgccgtgt tgctgggctt cggcgcgacg gctatttatc cataccttgc ctatgaaacg
    65041 ctgggccgcc tggttgacac ccatgcgatt gccaaagatt atcgtaccgt gatgctcaac
    65101 taccgtaacg gcatcaacaa aggcttgtac aaaatcatgt ccaaaatggg catctccacc
    65161 atcgcctctt accgctgctc gaaactgttt gaagcggtcg gtctgcacga tgatgtagtg
    65221 ggcctgtgct tccagggggc ggtcagccgc attggtgggg cgagctttga agacttccag
    65281 caggatctgc tgaacctgtc gaaacgtgcc tggctggcgc gtaagcccat cagccagggc
    65341 ggcctgctga aatacgtcca cggcggcgaa taccacgctt acaacccgga cgtggtgcgc
    65401 acgctgcaac aggcggtaca aagcggcgag tacagcgact atcaggaata cgcgaagctg
    65461 gttaatgagc gtccggcaac cacgctgcgc gatctgctgg caattacgcc gggtgaaaac
    65521 gcggtcaaca ttgctgatgt tgaaccggca agcgaactgt ttaaacgctt tgataccgcc
    65581 gcgatgtcta tcggcgcgtt aagcccggaa gcccacgagg cgctggcgga agcgatgaat
    65641 agcatcggcg gtaactcgaa ctccggtgaa ggcggcgaag acccggcgcg ctatggcact
    65701 aacaaagtgt cgcgcatcaa gcaggtggct tctggtcgct tcggcgttac tccggcgtat
    65761 ctggtcaatg ccgacgtcat tcagattaaa gtcgcccagg gcgcgaagcc gggcgaaggc
    65821 ggtcagttgc cgggcgacaa agtcactcct tacatcgcca aactgcgcta ttcggtgccc
    65881 ggagtgacgc tgatctcccc gccgccgcac cacgatatct actctatcga ggacttagcg
    65941 cagctcattt tcgacctcaa gcaggttaac ccgaaagcga tgatctccgt gaagctggtt
    66001 tccgaaccgg gcgtaggcac catcgcgact ggcgtggcaa aagcttatgc cgatttaatc
    66061 accatcgcag gctatgacgg tggcaccggc gcaagcccgc tgtcgtcagt gaaatacgca
    66121 ggttgcccgt gggagctggg gcttgttgaa acccagcagg cgctggttgc caatggtctg
    66181 cgtcataaga tccgtttgca ggtcgatggc ggcctgaaaa ccggtgttga tatcatcaaa
    66241 gcggcgattc tcggcgcaga aagcttcggc ttcggcactg gcccaatggt cgcgctcggc
    66301 tgtaaatatc tgcgtatttg ccacctgaac aactgcgcaa cgggtgtggc aactcaggat
    66361 gacaaactgc gtaagaatca ctatcacggc ctgccgttca aggtgacgaa ttactttgag
    66421 tttatcgccc gtgaaacccg cgagctgatg gcacagctgg gggtaacgcg gctggtggat
    66481 ctgattggtc gcaccgacct gctgaaagag ctggacggtt tcaccgccaa acagcagaaa
    66541 ctggcgctgt cgaagctgct ggagaccgcc gaaccgcatc caggtaaggc actctactgc
    66601 accgaaaaca acccgccgtt tgataacggc ctgctgaacg cgcagttgct gcaacaggcg
    66661 aaaccgtttg tcgatgaacg ccagagcaaa accttctggt tcgatatccg caataccgat
    66721 cgctccgtgg gcgcgtcgct ttcaggctat atcgcccaga cgcacggcga tcaggggctg
    66781 gcagccgatc ctatcaaagc gtacttcaac ggcaccgcag gccagagttt cggtgtatgg
    66841 aacgcgggcg gcgtggaact gtacctgacc ggggatgcca acgactatgt cggtaaaggc
    66901 atggcgggcg gcttaatcgc cattcgtcct ccggttggtt ccgccttccg cagccatgaa
    66961 gcaagtatta tcggcaacac ctgcctgtat ggcgcgaccg gtggtcgtct gtatgccgca
    67021 ggccgcgcgg gtgaacgttt cggcgtgcgg aactccggtg ctatcaccgt ggtagaaggc
    67081 attggcgaca acggctgtga atatatgacg ggcggtattg tctgtattct gggtaaaacc
    67141 ggcgttaact tcggtgcggg catgaccggc ggcttcgctt acgttctcga tgaaagcggt
    67201 gatttccgca aacgcgttaa cccggaactg gtcgaggtct taaacgttga cgatctggcg
    67261 atccatgaag agcatctgcg cggtcttatc accgagcatg ttcagcatac tggctctcag
    67321 cgcggtgaag agattctggc gaactggtca accttcgcca ctaaatttgc gctggttaaa
    67381 ccgaagtcca gtgatgtaaa agcactgctg ggtcaccgta gtcgtagcgc agcagagttg
    67441 cgcgtgcagg cgcagtaagg ggtagcaaca atgagtcaga atgtttatca atttatcgac
    67501 ctgcagcgcg ttgatccgcc taagaaaccg ctgaagatcc gcaaaattga gtttgttgaa
    67561 atttacgagc agttttccga aggccaggcc aaagcgcagg ctgaccgctg cctgtcgtgc
    67621 ggcaacccat actgcgagtg gaaatgtccg gtacacaact acatcccgaa ctggctgaag
    67681 ctcgccaacg aggggcgtat ttttgaagcg gcggaactgt cgcaccagac caacaccctg
    67741 ccggaagttt gcggacgagt ctgcccgcaa gaccgtctgt gcgaaggttc ctgcactctg
    67801 aacgatgagt ttggcgcggt gaccatcggc aacattgagc gttatatcaa tgataaagcg
    67861 ttcgaaatgg gctggcgtcc ggatatgtcc ggcgtgaagc aaaccggtaa aaaagtggcg
    67921 attatcggcg caggcccggc aggtctggcg tgtgcggatg tcctgacgcg taacggcgta
    67981 aaagccgttg tcttcgaccg tcatccagaa attggcgggc tgctgacctt cggtattccg
    68041 gccttcaagc tggaaaaaga ggtaatgacg cgtcgccgtg aaatcttcac cggcatgggt
    68101 attgaattca aactcaatac cgaagtgggc cgcgacgtgc agctggacga tctgctgagt
    68161 gattacgatg ccgtcttcct tggcgtcggg acttatcagt caatgcgcgg cggtctggaa
    68221 aacaaagacg ccgatggcgt gtacgcagcg ctgccgttcc ttatcgccaa caccaaacag
    68281 ttaatgggct ttggtgaaac ccgcgacgaa ccgttcgtca gcatggaagg caaacgcgtg
    68341 gtggtccttg gcggtggcga cactgcgatg gactgcgtgc gtacgtccgt gcgccaggga
    68401 gcgaagcacg ttacctgtgc ctatcgtcgt gatgaagaga acatgccggg ttcccgccgc
    68461 gaagtgaaaa acgcgcggga agaaggcgta gagttcaaat tcaacgtcca gccactgggg
    68521 attgaagtga acggtaacgg caaagtcagc ggcgtaaaaa tggtacgtac cgaaatgggc
    68581 gaaccggatg ccaaaggccg tcgccgcgcg gagatcgttg caggttccga acatatcgtt
    68641 ccggcagatg cagtgatcat ggcgtttggt ttccgtccac acaacatgga atggctggca
    68701 aaacacagcg tcgagctgga ttcacaaggc cgcatcatcg ccccggaagg cagcgacaac
    68761 gccttccaga ccagcaaccc gaaaatcttt gctggcggcg atatcgtccg tggttccgat
    68821 ctggtggtaa ccgctattgc cgaaggtcgt aaggcggcag acggtattat gaactggctg
    68881 gaagtttaag ctctgtccca gcgccgcctc ctcagaaaag agtcggcgct gaaattctcc
    68941 tttctttctg gaaagcgcct cgcataatct gaagaaaacc aatgtaatac gctcatctgg
    69001 attgttaccg gtagatgccc ggcgtatagc tgattccatt attcaacgca gcgccatata
    69061 aagggacaga taatggaatc gctctcagag agaacctcaa caggctacca gcaaatccac
    69121 gacggtatta ttcacctggt cgatagcgcc cggacggaaa cggtacgtag cgttaacgcg
    69181 ctgatgaccg cgacatactg ggaaattggc cgacgaattg tcgaatttga acaaggtggc
    69241 gaggcaaggg ctgcgtatgg tgcgcagcta atcaagcgac tatcaaagga tttaagtcta
    69301 aggtataagc gtgggttctc tgcaaaaaac ttacgccaaa tgaggctttt ttacctcttt
    69361 tttcaacatg ttgaaattcg ccag
//
LOCUS       DAJVUJ010000005        66348 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.005, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000005 DAJVUJ010000000
VERSION     DAJVUJ010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 66348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66348
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.005"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(129..608)
                     /gene="folK"
                     /locus_tag="OQQ48_000331"
     CDS             complement(129..608)
                     /gene="folK"
                     /locus_tag="OQQ48_000331"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HCS1951220.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQDRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDELSKW"
     gene            complement(605..2023)
                     /gene="pcnB"
                     /locus_tag="OQQ48_000332"
     CDS             complement(605..2023)
                     /gene="pcnB"
                     /locus_tag="OQQ48_000332"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HCS1951221.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTA"
     gene            complement(2062..2988)
                     /gene="gluQRS"
                     /locus_tag="OQQ48_000333"
     CDS             complement(2062..2988)
                     /gene="gluQRS"
                     /locus_tag="OQQ48_000333"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HCS1951222.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIVGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQVEAHWQDFS
                     VEQILQSAVKNWTLTAVPESAIVNSTFSNASC"
     gene            complement(3025..3480)
                     /gene="dksA"
                     /locus_tag="OQQ48_000334"
     CDS             complement(3025..3480)
                     /gene="dksA"
                     /locus_tag="OQQ48_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HCS1951223.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3658..4362)
                     /gene="sfsA"
                     /locus_tag="OQQ48_000335"
     CDS             complement(3658..4362)
                     /gene="sfsA"
                     /locus_tag="OQQ48_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HCS1951224.1"
                     /translation="MEFSPLLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATRGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMNVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4377..4907)
                     /gene="thpR"
                     /locus_tag="OQQ48_000336"
     CDS             complement(4377..4907)
                     /gene="thpR"
                     /locus_tag="OQQ48_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HCS1951225.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALMQ"
     gene            4981..7410
                     /gene="hrpB"
                     /locus_tag="OQQ48_000337"
     CDS             4981..7410
                     /gene="hrpB"
                     /locus_tag="OQQ48_000337"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HCS1951226.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLEELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDDAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIARRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGLLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQSPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7606..10128
                     /gene="mrcB"
                     /locus_tag="OQQ48_000338"
     CDS             7606..10128
                     /gene="mrcB"
                     /locus_tag="OQQ48_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HCS1951227.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRAHLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGLVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTDTWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWAGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10348..12537
                     /gene="fhuA"
                     /locus_tag="OQQ48_000339"
     CDS             10348..12537
                     /gene="fhuA"
                     /locus_tag="OQQ48_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HCS1951228.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAAKHSATATKTDTPIEKTPQSISVVTNEEMQMHQFQSVKEA
                     LGYTPGVTVSSRGASNTYDFVIIRGFSSVGLNQNNYLDGLKLQGNFYNDAVIDPYMLE
                     RVELMRGPTSVLYGKSNPGGIISMVSKRPTTEPLKEIQFKMGTDNLFQTGFDFSDALD
                     DNGEFSYRLTGLARSTNEQQKNSESQRYTIAPSFSWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGASNNTYSRNQKMVGYSFEHGFNDTFTVRQNLR
                     FSEMKTSQKSVYGTGIAGDGHTLNRGTVVDNERLQNFSVDTQLESKFATGDVEHTLLT
                     GVDFMRMRNDINASFGSAPSIDLYNKYHPEYFAFGSAEPYQMNESKQTGIYVQDQAEW
                     NKWVFTLGGRYDWSKQATTVRENSYTPTEGYIERNDHQFTWRGGVNYLFDNGISPYFS
                     YSQSFEPSAFDLWSNPRISYKPSKGEQYEAGVKYVPNDMPVVVTGAVYQLTKTNNLTA
                     DPTNPLAQVPAGEIRARGVELEAKAALNANINLTASYTYTDAEYTKDTNLKGKTPEQV
                     PEHMASLWGDYTFNEGPLSGLTLGTGGRFIGSSYGDPANTFKVGSAAVMDAVVKYDLA
                     RFGMAGSSLAVNVNNLLDREYVASCFQTYGCFWGAERQVVATATFRF"
     gene            12588..13385
                     /gene="fhuC"
                     /locus_tag="OQQ48_000340"
     CDS             12588..13385
                     /gene="fhuC"
                     /locus_tag="OQQ48_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HCS1951229.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSSKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGDTLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13385..14275
                     /gene="fhuD"
                     /locus_tag="OQQ48_000341"
     CDS             13385..14275
                     /gene="fhuD"
                     /locus_tag="OQQ48_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HCS1951230.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSAEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14272..16254
                     /gene="fhuB"
                     /locus_tag="OQQ48_000342"
     CDS             14272..16254
                     /gene="fhuB"
                     /locus_tag="OQQ48_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HCS1951231.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWMWASGALLDDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSIGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLYRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATSTMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16289..17569)
                     /gene="hemL"
                     /locus_tag="OQQ48_000343"
     CDS             complement(16289..17569)
                     /gene="hemL"
                     /locus_tag="OQQ48_000343"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HCS1951232.1"
                     /translation="MSKSENLYSAARELLPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPQPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGTPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17794..19215
                     /gene="clcA"
                     /locus_tag="OQQ48_000344"
     CDS             17794..19215
                     /gene="clcA"
                     /locus_tag="OQQ48_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HCS1951233.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMEALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVVVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSEILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19239..19304
                     /locus_tag="OQQ48_000345"
     CDS             19239..19304
                     /locus_tag="OQQ48_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1951234.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            19297..19641
                     /gene="erpA"
                     /locus_tag="OQQ48_000346"
     CDS             19297..19641
                     /gene="erpA"
                     /locus_tag="OQQ48_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HCS1951235.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19688..20311)
                     /locus_tag="OQQ48_000347"
     CDS             complement(19688..20311)
                     /locus_tag="OQQ48_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HCS1951236.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20349..21148)
                     /gene="btuF"
                     /locus_tag="OQQ48_000348"
                     /pseudo
     CDS             complement(20349..21148)
                     /gene="btuF"
                     /locus_tag="OQQ48_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
     gene            complement(21141..21839)
                     /gene="mtnN"
                     /locus_tag="OQQ48_000349"
     CDS             complement(21141..21839)
                     /gene="mtnN"
                     /locus_tag="OQQ48_000349"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCS1951237.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHGKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21923..23440
                     /gene="dgt"
                     /locus_tag="OQQ48_000350"
     CDS             21923..23440
                     /gene="dgt"
                     /locus_tag="OQQ48_000350"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HCS1951238.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRMKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRYRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23570..24994
                     /gene="degP"
                     /locus_tag="OQQ48_000351"
     CDS             23570..24994
                     /gene="degP"
                     /locus_tag="OQQ48_000351"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HCS1951239.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25149..26306
                     /gene="cdaR"
                     /locus_tag="OQQ48_000352"
     CDS             25149..26306
                     /gene="cdaR"
                     /locus_tag="OQQ48_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HCS1951240.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGRLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIYRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(26395..26781)
                     /locus_tag="OQQ48_000353"
     CDS             complement(26395..26781)
                     /locus_tag="OQQ48_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HCS1951241.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(26943..27755)
                     /gene="yaeI"
                     /locus_tag="OQQ48_000354"
     CDS             complement(26943..27755)
                     /gene="yaeI"
                     /locus_tag="OQQ48_000354"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase YaeI"
                     /protein_id="HCS1951242.1"
                     /translation="MISRRRLLQATAATIATSSGFGYMHYWEPGWFELIRHRLAFFKD
                     NAAPFKILFLADLHYSRFVPLSLISDAIALGIEQKPDLILLGGDYVLFDMPLNFSAFS
                     DVLSPLAECAPTFACFGNHDRPVGTEKNHLIGETLKSVGITVLFNQATVIATPNRQFE
                     LVGTGDLWAGQCKPPPASEANLPRLMLAHNPDSKEVMRDEPWDLMLCGHTHGGQLRVP
                     LVGEPFAPVEDKRYVAGLNAFGERHIYTTRGVGSLYGLRLNCRPEVTMLELV"
     gene            complement(27810..28634)
                     /gene="dapD"
                     /locus_tag="OQQ48_000355"
     CDS             complement(27810..28634)
                     /gene="dapD"
                     /locus_tag="OQQ48_000355"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HCS1951243.1"
                     /translation="MQQLQNIIETAFERRAEITPTNADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGHVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(28665..31337)
                     /gene="glnD"
                     /locus_tag="OQQ48_000356"
     CDS             complement(28665..31337)
                     /gene="glnD"
                     /locus_tag="OQQ48_000356"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HCS1951244.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(31399..32193)
                     /gene="map"
                     /locus_tag="OQQ48_000357"
     CDS             complement(31399..32193)
                     /gene="map"
                     /locus_tag="OQQ48_000357"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCS1951245.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            32561..33286
                     /gene="rpsB"
                     /locus_tag="OQQ48_000358"
     CDS             32561..33286
                     /gene="rpsB"
                     /locus_tag="OQQ48_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCS1951246.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFEKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            33544..34395
                     /gene="tsf"
                     /locus_tag="OQQ48_000359"
     CDS             33544..34395
                     /gene="tsf"
                     /locus_tag="OQQ48_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCS1951247.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            34542..35267
                     /gene="pyrH"
                     /locus_tag="OQQ48_000360"
     CDS             34542..35267
                     /gene="pyrH"
                     /locus_tag="OQQ48_000360"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCS1951248.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            35559..36116
                     /gene="frr"
                     /locus_tag="OQQ48_000361"
     CDS             35559..36116
                     /gene="frr"
                     /locus_tag="OQQ48_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCS1951249.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            36208..37404
                     /gene="ispC"
                     /locus_tag="OQQ48_000362"
     CDS             36208..37404
                     /gene="ispC"
                     /locus_tag="OQQ48_000362"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HCS1951250.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            37592..38350
                     /gene="ispU"
                     /locus_tag="OQQ48_000363"
                     /gene_synonym="uppS"
     CDS             37592..38350
                     /gene="ispU"
                     /locus_tag="OQQ48_000363"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HCS1951251.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQECIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFIDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            38363..39220
                     /gene="cdsA"
                     /locus_tag="OQQ48_000364"
     CDS             38363..39220
                     /gene="cdsA"
                     /locus_tag="OQQ48_000364"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCS1951252.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVSLLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            39232..40584
                     /gene="rseP"
                     /locus_tag="OQQ48_000365"
     CDS             39232..40584
                     /gene="rseP"
                     /locus_tag="OQQ48_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HCS1951253.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GLQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSLLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGAHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            40614..43046
                     /gene="bamA"
                     /locus_tag="OQQ48_000366"
     CDS             40614..43046
                     /gene="bamA"
                     /locus_tag="OQQ48_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HCS1951254.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            43168..43653
                     /gene="skp"
                     /locus_tag="OQQ48_000367"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             43168..43653
                     /gene="skp"
                     /locus_tag="OQQ48_000367"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HCS1951255.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            43657..44682
                     /gene="lpxD"
                     /locus_tag="OQQ48_000368"
     CDS             43657..44682
                     /gene="lpxD"
                     /locus_tag="OQQ48_000368"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HCS1951256.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44787..45242
                     /gene="fabZ"
                     /locus_tag="OQQ48_000369"
     CDS             44787..45242
                     /gene="fabZ"
                     /locus_tag="OQQ48_000369"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCS1951257.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            45246..46034
                     /gene="lpxA"
                     /locus_tag="OQQ48_000370"
     CDS             45246..46034
                     /gene="lpxA"
                     /locus_tag="OQQ48_000370"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HCS1951258.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVGIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFSRSTRGLIR"
     gene            46034..47182
                     /gene="lpxB"
                     /locus_tag="OQQ48_000371"
     CDS             46034..47182
                     /gene="lpxB"
                     /locus_tag="OQQ48_000371"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HCS1951259.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDFSVHLLDGMGREAMVASDAALLASG
                     TAALECMLVKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKISHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            47179..47775
                     /gene="rnhB"
                     /locus_tag="OQQ48_000372"
     CDS             47179..47775
                     /gene="rnhB"
                     /locus_tag="OQQ48_000372"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCS1951260.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALCEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMVVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            47812..51294
                     /gene="dnaE"
                     /locus_tag="OQQ48_000373"
     CDS             47812..51294
                     /gene="dnaE"
                     /locus_tag="OQQ48_000373"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCS1951261.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGVNAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYASGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            51307..52266
                     /gene="accA"
                     /locus_tag="OQQ48_000374"
     CDS             51307..52266
                     /gene="accA"
                     /locus_tag="OQQ48_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HCS1951262.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSSYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            52365..54474
                     /gene="ldcC"
                     /locus_tag="OQQ48_000375"
                     /pseudo
     CDS             52365..54474
                     /gene="ldcC"
                     /locus_tag="OQQ48_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
     gene            54531..54920
                     /locus_tag="OQQ48_000376"
     CDS             54531..54920
                     /locus_tag="OQQ48_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCS1951263.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54985..56283
                     /gene="tilS"
                     /locus_tag="OQQ48_000377"
     CDS             54985..56283
                     /gene="tilS"
                     /locus_tag="OQQ48_000377"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCS1951264.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELAQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSREALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKSAGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(56332..56592)
                     /gene="rof"
                     /locus_tag="OQQ48_000378"
     CDS             complement(56332..56592)
                     /gene="rof"
                     /locus_tag="OQQ48_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HCS1951265.1"
                     /translation="MLMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(56579..56779)
                     /locus_tag="OQQ48_000379"
     CDS             complement(56579..56779)
                     /locus_tag="OQQ48_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HCS1951266.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56945..57490
                     /locus_tag="OQQ48_000380"
     CDS             56945..57490
                     /locus_tag="OQQ48_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HCS1951267.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            57487..57909
                     /gene="arfB"
                     /locus_tag="OQQ48_000381"
     CDS             57487..57909
                     /gene="arfB"
                     /locus_tag="OQQ48_000381"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCS1951268.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSAKAMRGKVRSGRE"
     gene            57923..58633
                     /gene="nlpE"
                     /locus_tag="OQQ48_000382"
                     /gene_synonym="cutF"
     CDS             57923..58633
                     /gene="nlpE"
                     /locus_tag="OQQ48_000382"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308221.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HCS1951269.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERGYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(58833..59653)
                     /locus_tag="OQQ48_000383"
                     /pseudo
     CDS             complement(58833..59653)
                     /locus_tag="OQQ48_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
     gene            complement(59706..61424)
                     /gene="proS"
                     /locus_tag="OQQ48_000384"
     CDS             complement(59706..61424)
                     /gene="proS"
                     /locus_tag="OQQ48_000384"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCS1951270.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(61534..62241)
                     /gene="tsaA"
                     /locus_tag="OQQ48_000385"
     CDS             complement(61534..62241)
                     /gene="tsaA"
                     /locus_tag="OQQ48_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HCS1951271.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYHKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(62238..62642)
                     /gene="rcsF"
                     /locus_tag="OQQ48_000386"
     CDS             complement(62238..62642)
                     /gene="rcsF"
                     /locus_tag="OQQ48_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HCS1951272.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(62760..63575)
                     /gene="metQ"
                     /locus_tag="OQQ48_000387"
     CDS             complement(62760..63575)
                     /gene="metQ"
                     /locus_tag="OQQ48_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HCS1951273.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(63615..64268)
                     /gene="metI"
                     /locus_tag="OQQ48_000388"
     CDS             complement(63615..64268)
                     /gene="metI"
                     /locus_tag="OQQ48_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HCS1951274.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(64261..65292)
                     /gene="metN"
                     /locus_tag="OQQ48_000389"
     CDS             complement(64261..65292)
                     /gene="metN"
                     /locus_tag="OQQ48_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HCS1951275.1"
                     /translation="MIKLSNITKVFHQGTRIIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            65480..66052
                     /gene="gmhB"
                     /locus_tag="OQQ48_000390"
     CDS             65480..66052
                     /gene="gmhB"
                     /locus_tag="OQQ48_000390"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HCS1951276.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFVLVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAAAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPA"
BASE COUNT        15765 a        16432 c        18202 g        15949 t
ORIGIN      
        1 atgggattga tatttatctg cgggaattta tattatgttt ctgcaaaaat gtttaaacta
       61 aaatttttat taaggaaaga gaggatgtat gattttcaga aaatatttat attcgtaata
      121 taaataaatt accatttgct taattcgtca aatgctcttg tatgtaagac ttcacgcaac
      181 gtctccccat caggaaacgc cagctccggt gcaatctcaa acagcggcca cagcataaat
      241 ccacgattct tcatatcgta gtgcggaacg gtcaggcgtt cggtatttat cacttcatta
      301 ccaaacagca tgatatcgag atccagcgtg cgtggtcccc agcgttcagc tttacgaaca
      361 cgatcctgtt gcagttctat gcgctgtgta tggttgagta actcttccgg ggcgagcgaa
      421 gtttcaagcg ccacggcggc gttgaggtaa tcgggttgat cttgcggccc cagcggtgga
      481 gtgcggtaaa acgaagaaac ggcaagaatg tggctttcag ggatatcgcc taatgctttc
      541 agggcagcat tgacctgctc cagcggagag gccagattgc tgcctatggc aatatacgcc
      601 actgtcatgc ggtaccctca cgacgcggtg cgcgtttgcg tggacgacga gtacggcgac
      661 gcggtgacgg ctcttcatcc aactcgttaa gcatcccttt ttgatctggt ggcgcggaaa
      721 cctggaactc accccaccat ttcaccagac gctgcagttc agcgttacgt tcaacttcag
      781 ctcgcaaggc caacaggtca taagccgcac ggaacttagg atgctccagc agtttccatg
      841 cgcgtttacc ctgacgacgg gacatacgca actgcaactg ccagatatcg cgggttaacg
      901 tcgtcagacg tttcgggatt gccagtgaac ggcaggcttc gtccagcacg tcgttcatcg
      961 ccagcgcgaa agcgtcgtga taggtcaggc cgctttcctg ggcgatcttc tgtgccgtct
     1021 ccagcagtgg gtaccagaac atggcggcaa acaagaacgc cgggttcacg cgcatatcgt
     1081 tatggatacg cgtatcggta ttcttcagca cctgttcaat gatccgctcc atcgggctgt
     1141 cgccattttc cgtgaagtag cgggtaatgg tcgggaacag cggctggaac agatgatatt
     1201 cacacaacag cttataggtt tcgtaaccgt agcccgcttg tagcagttta agcgattctt
     1261 caaacaggcg tgccggtggg atatcgttca gcagggtagc gaggcgaggg atcggttctg
     1321 cggtttccgg gctgatgcgc atacccaatt tggcggcaaa acgtaccgcg cgcagcatac
     1381 gtaccggatc ttcacggtag cgcgtttccg ggttaccaat cagacggata acgccgtcct
     1441 tcagatcctt catgccgcca acgtaatcac ggacggtaaa atccgctacg ctgtaataca
     1501 ggctgttgat agtgaaatcg cggcgctggg cgtcttcttc gatggagccg aaaatgttgt
     1561 cgcgcagcaa catgccgttt tgcccgcgtt gggaggtcgt gcggtcgctg acgttacctt
     1621 cgtggtgtcc acggaaggtt gcaacttcga taatctccgg gccaaacatc acatgagcca
     1681 gacggaaacg gcgacccaca aggcggcagt tacggaacag tttgcgcacc tgctcaggcg
     1741 tggcgttagt ggttacgtca aaatctttcg gctttttgcc aagtaacagg tcgcgcacgc
     1801 cgccgccaac cagccaggct tcgtatcccg ctttattgag cctgtacatt accttcaggg
     1861 cattttcact gatatctttg cgggaaatag catgctgctc acgcgggatc accgtcacct
     1921 gtggacgggc gactgcctgt tcagcctcgc tttcctcgcg gcttagcacc ttgcggcaaa
     1981 aattagcgac tcgggtaaaa atagtacacc tcggtagtgt caaacatcat tcaggacaaa
     2041 aaaatagcgg ctaatcatag ctcagcatga cgcatttgag aatgttgaat ttacaattgc
     2101 cgactcgggc acggcggtca gcgtccagtt tttgacggct gactgaagga tttgctcgac
     2161 gctgaaatcc tgccagtgtg cttctacctg ctgccccaga aattgaagtg ccgcgattag
     2221 taccgggcgt ggatcgcctt tcggcaacgc aggcgcatga ttctgcttgg aaagtttagc
     2281 gccttgtgga ttaagcgcca gcggcagatg aatgtaatct ggcactttcc agccaaaaag
     2341 ctggtacagc gagatttgcc ttactgttgg ttcaatcaga tcagccccac gcactatttc
     2401 tgtaacgccc tggaaatgat catcaaccac aacagccagg ttgtaggcga acaacccatc
     2461 acggcgatga atgataaaat cttcccgtgc cagtttttcg tcggcgtgaa taatgccgcg
     2521 cagctggtca gtaaattgcg tgaccggatg ctgctggcgg atacgcactg cggcgttgtc
     2581 tggtccatga tgcaacaccc ggcaatgacc gtcgtaaata ccgacaatgc tttgaatacg
     2641 cgcacgcgta caggtgcagt aataacttag tccttgttca tgtaaccagg cgagtgcttc
     2701 acgataggcg tcgtgacgtt gcgattgcca gagaacatcg ccgtcccagt gcagaccgta
     2761 atgttccagc tggcgcagga tagtttctgc ggcaccggga acttcacgag gcgggtcgat
     2821 atcttctatg cgtaccagcc agcgaccttg ccgggcgcga gcctgcaaat agctgccgag
     2881 cgcggcgatc agagagccaa aatgaagctc gccggaagga gagggggcga agcggccaat
     2941 atactgtgtg tctgtcatct ctttgaacaa aaaataaggc gggagcattt cccgcctgtg
     3001 gtaaacgtga tggaacggct gtaattagcc agccatctgt ttttcgcgaa tttcagccag
     3061 cgttttgcag tcgatgcaca gatcggctgt cgggcgcgct tccagacggc gaataccaat
     3121 ttcaacaccg caggattcgc agtagccgaa atcttcgtct tccacttttt tcagcgtctt
     3181 ctcgatcttt ttgatcagct tacgctcgcg atcgcggtta cgcagttcga ggctgaactc
     3241 ttcttcctgg gctgcacggt ctaccgggtc cgggaagttg gctgcttcat cctgcatatg
     3301 tgtaacggtg cgatcgactt catccctgag ttgattacgc catgcttcca gaatacgacg
     3361 gaagtgcgcc agctgggctt cattcatata ctcttcgccc ggcttctcct gatatggttc
     3421 caccccagcg atggcgagaa tactcaggga cgatgtttta cggttttgcc cttcttgcat
     3481 gttgcttctc cttaacacgc actatcgatc cccatgttcg ggggaaaaat gaggccgcta
     3541 taaatagcag atgcttttcc ggatagcaat tatctaaacg taacacttga caactgtgtg
     3601 aggaaaagcg tatttgcgca cgcgaccaga atgtaaatta accagttact tactttacta
     3661 caatgtaacc ggcagtgatt ttttaagagc catgccttca gcagaaattt ccgctttgta
     3721 agccagaatt tctacccccc tctgttgagc ttctgacaat agttgcgcgt atttctcatc
     3781 gatgtggcgc gcgggtgaaa accgtgtaat ggctgaatgc agcacggcga aaaagataac
     3841 cgcacgctgg ccttcagccg ctacgttcat caactcccga aggtgtttct gacctcgttc
     3901 agtgaccgca tcgggaaaat atccctgttc gttctccgct aacgtaaccg atttcacttc
     3961 aatatagcag tctggacgcg aatccgcctg caacataaag tcaatacggc tgcgttctgc
     4021 gccatatttt acttcgcttt tcagcgagct atagcctgac agttctgaaa ttgattcatt
     4081 aaggatagcc tctttcgtca acctgttagc ccaaagcgta ttgacgcaaa taaatgcgcc
     4141 gctctggctt tgagttagtt cccaggtgtg tgggtatttc cgtttggtgt tgtctgaagt
     4201 cgaataccag acggtatcgc cacgcgttgc acaaccggtc atcgcacccg tattcgggca
     4261 gtgtagcgtt aattcgcgac catcgggtgt gatcacatcg gctaaaaaac gtttgtaacg
     4321 ctgaattagc gtcgcgcgct gtagaagggg agagaattcc attgcgacaa tccttgttat
     4381 tgcattagcg cccagcgttt tagcggcgtg tagcgtgtgc gtccacgggc aaacgaggag
     4441 gcgtaaaggg tgaactccgt caccgcataa gaccagttaa aacctggcgg cgggattgtc
     4501 accgcctcgc tggcgtcgcg caataaggta atatgtggat gaaacggacg attgctttga
     4561 aaacaaccgc tgcgggcagc ctgtgaacgg agcatattcg ccagctggat taagccgcgt
     4621 ggcggctgac gcatccctaa ccacaccaca cgcgaacgca gccattgtcc ggcgtcatca
     4681 agcgtgagtg tgaaaccagg ttgacgaatc cgtccggcta aaagagaaag cgccttctct
     4741 ttctcggcgc tcacttcgcc taaaaatgcc agagtcagat gcaaattatc ggcggcgacc
     4801 ggacgtcccg cctcaggtgg gaagtgtgtg gcgcgccagt ggataatctg ttcgcggatt
     4861 tctgcaggta agtcgatagc aaagaacaga cgttgcggtt cagacatgtg aggcactcgg
     4921 ttatgaatta ccgcgatgct acaatgtggc gcgaagaatg ttaaccctct ggagcgtttt
     4981 gtgtcgtcgt tgcccgttgc tgccgtttta cctgaattac ttaccgctct cgattgtgcg
     5041 ccgcaggtat tattaagtgc gccgaccggg gccgggaaat caacctggct gccgctgcaa
     5101 ctgctggcgc atcccggcat taacgggaaa attatcctgc tggagccgcg tcgtctggcg
     5161 gcgcgtaacg tcgcgcaacg gctggaggag ctgcttaacg aaaagccagg cgataccgtt
     5221 ggctaccgga tgcgtgcgca aaactgcgtc gggccgaata cccgcctgga agtagttacc
     5281 gaaggcgtgc tgacgcggat gatccagcgt gacccggagt tgagcggtgt tggattggtg
     5341 atccttgatg aatttcatga gcgtagcttg caggcggatt tggcgttggc gctgttactc
     5401 gatgtgcaac aaggtctgcg tgatgacctt aaactgctaa ttatgtcggc tacgctggac
     5461 aacgaccgct tgcagcaaat gctgccagaa gcgcccgtcg tcatctcaga agggcgctcg
     5521 tttccggttg aacgccgtta tttaccgctg cccgcgcatc agcgttttga cgatgccgtt
     5581 gcggtagcca ccgctgaaat gctgcgtcag gaaagcggat cattactgtt atttttacct
     5641 ggcgtcggag aaattcagcg tgtgcaggaa caactggctt cgcgcatcgg cagtgatgta
     5701 ttgctctgcc cgctgtatgg cgcgttgtcg ctgaacgatc agcgaaaagc gatcctcccg
     5761 gcaccgcaag ggatgcgcaa agtggtgctg gcgaccaata ttgctgaaac cagtttaacc
     5821 atcgaaggta ttcgtctggt ggtggattgt gcccaggagc gtgtggcgcg ttttgatccg
     5881 cgcacggggc taacgcgact gattactcaa cgcgttagcc aggcatccat gacgcagcgt
     5941 gccgggcgcg ccgggcgtct ggagccgggt atctgcctgc atctaatcgc caaagaacaa
     6001 gcagaacgcg ctgccgcgca aagtgaaccg gaaatcttac aaagcgatct ttccggtttg
     6061 ctgatggaat tactgcaatg gggatgcagc gatccggcgc agatgagctg gctggatcaa
     6121 ccgccaacgg tgaatctact ggccgcgaaa cgcctgttac aaatgctggg ggcgctggag
     6181 ggtgaacggc ttagtgcgca ggggcaaaaa atggcagcgc tgggtaacga tccgcgttta
     6241 gcggcaatgc tggttagcgc gaagaacgac gacgaagctg ctaccgcggc aaaaattgcc
     6301 gccattctcg aagagccgcc acggatgggc aatagtgacc tgggcgtggc gttttcgcgc
     6361 aatcaaccag cctggcagca acgtagtcag caactgttaa aacgcttaaa cgtacgtggc
     6421 ggtgaggcag acagttcgct tatcgcgccg ctacttgccg gggcgtttgc cgatcgcatt
     6481 gctcgtcgcc gtgggcaaga tggacgctat caactggcaa acggcatggg agcgatgctc
     6541 gatgccgacg acgcgctaag ccgccacgaa tggttgatcg caccgttatt attgcagggc
     6601 agcgcctcgc cggatgcgcg gattttactg gcgctgccgg tcgatattga tgagttagta
     6661 caacgctgcc cgcagctggt acagcagtct gacactgtgg agtgggatga cgcgcaaggt
     6721 acgctgaaag cctggcgtcg gctgcaaatc ggtctgctga cggtgaaagt gcagccgctg
     6781 gcgaaaccct cggaagacga gttgcatcag gcgatgctta atggcatccg tgataaaggt
     6841 ttaagcgtgc tcaactggac ggcggaagcg gaacagctac gcttgcgttt gttatgcgcc
     6901 gcaaagtggt tgccggaata tgactggcca gcggttgatg atgaaagttt attggcgacg
     6961 ctggaaacgt ggctgctgcc acatatgact ggcgtacatt cactacgcgg cctgaaatca
     7021 ctcgacattt atcaggcact acgcggatta cttgattggg gaatgcagca acgtctggat
     7081 agtgaattgc ctgcgcatta cactgtgccg acgggaagcc ggatcgccat tcgttatcat
     7141 gaagataacc cgcccgcgct ggcggtgaga atgcaagaga tgtttggcga ggccaccaat
     7201 ccgacgatcg cccaggggcg cgtgccgctg gtgctggagt tgctttcacc tgcccaaagt
     7261 ccattacaaa tcacacgaga tttgagcgcc ttctggaaag gagcgtaccg tgaggtgcaa
     7321 aaagagatga aagggcgtta tcccaaacat gtctggccgg acgacccggc aaatactgca
     7381 ccgacgcgac ggacgaaaaa gtattcgtaa ggttggtttt ctccctctcc ctgtgggaga
     7441 gggccggggt gagggcatca gcgcgcacgt tcaccctcac cctaaccctc tccctcaagg
     7501 gagaggggac cgatcgaaca caaattttga gagatatctt cttctgtctt gtaacagaag
     7561 aacagaaaat cgggcttttg cgcctgaata ttgcggagaa aaagcatggc cgggaatgac
     7621 cgcgagccaa ttggacgcaa agggaaaccg acgcgtccgg tcaaacaaaa ggtaagccgt
     7681 cgtcgttacg aagatgacga tgattacgac gattatgatg actatgagga tgaagaaccg
     7741 atgccgcgca aaggtaaggg caaaggcaaa gggcgtaagc ctcgtggcaa acgcggctgg
     7801 ctatggctac tgctaaaact ggctatcgtt tttgccgtgc tgatcgccat ttacggcgtt
     7861 tatctcgatc aaaaaattcg tagccgtatt gatggcaagg tctggcaact gcctgcagca
     7921 gtttatggcc gaatggtcaa tcttgagcca gatatgacca tcagcaagaa cgagatggtg
     7981 aagctgctgg aggcgactca gtatcgtcag gtgtcgaaaa tgacccgtcc tggcgaattt
     8041 accgtgcagg ccaacagcat tgagatgatc cgccgtccgt ttgatttccc ggacagtaaa
     8101 gaagggcagg tgcgtgcgca tctgaccttt gatggtgatc atctggcgac catcgtcaat
     8161 atggagaaca accgtcagtt cggctttttc cgtcttgatc cgcgtctgat caccatgatc
     8221 tcctcgccaa acggtgagca acgtctgttt gtgccgcgca gtggtttccc tgatttgctg
     8281 gtggatactt tgctggcgac agaagaccgc catttctacg agcatgatgg gatcagcctc
     8341 tactcaattg ggcgtgcggt gctggcaaac ctgaccgcag gtcgcacggt gcagggtgcg
     8401 agtacgctga cgcaacagct ggtgaaaaac ctgttcctct ccagcgagcg ctcttactgg
     8461 cgtaaagcga acgaagccta catggcgctg atcatggacg cgcgttacag caaagaccgt
     8521 attcttgagc tgtatatgaa cgaggtgtat ctcggtcaga gcggcgacaa cgaaatccgc
     8581 ggcttcccgc tggcaagctt gtattacttt ggtcgcccgg tagaagagct aagcctcgac
     8641 cagcaggcgc tgttagtcgg tatggtgaaa ggggcgtcca tctacaaccc gtggcgtaac
     8701 ccgaaactgg cgctggagcg acgtaatctg gtgctgcgtc tgctgcaaca gcaacagatt
     8761 attgatcaag aactctatga catgttgagt gcccgtccgc tgggggttca gccgcgcggt
     8821 ggggtgatct ctcctcagcc agcctttatg caactggtgc gtcaggagct gcaggcaaaa
     8881 ctgggcgata aggtaaaaga tctctccggc gtgaagatct tcactacctt tgactcggtg
     8941 gcccaggacg cggcagaaaa agccgccgtg gaaggcattc cggcactgaa gaaacagcgt
     9001 aagttgagcg atcttgaaac tgcgattgtg gtcgtcgacc gctttagtgg tgaagttcgt
     9061 gcgatggtgg gaggttctga gccgcagttt gcgggctaca accgtgcgat gcaggcgcgt
     9121 cgttcgattg gttcccttgc aaaaccagcg acttatctga cggctttaag ccagccgaaa
     9181 atctatcgcc tgaacacgtg gattgccgat gcgccaatcg cgctgcgtca gccgaatggc
     9241 ctggtctggt caccgcagaa cgatgaccgt cgttatagcg aaagcggcag agtgatgctg
     9301 gtggatgcgt tgacccgttc gatgaacgtg ccgacggtaa atctggggat ggcgctgggg
     9361 ctgcctgcag ttacggatac ctggatcaaa ctgggcgtac cgaaagatca gctgcatccg
     9421 gttccggcga tgctgctggg ggcgttgaac ttaacgccaa tcgaagtggc gcaggcgttc
     9481 cagaccatcg ccagcggtgg taaccgtgca ccgctttctg cactgcgttc ggtaatcgcg
     9541 gaagatggca aggtgctgta tcagagcttc ccgcaggcag aacgcgctgt tccggcgcag
     9601 gcggcgtatc tgacactatg gaccatgcag caggtggtac aacgtggtac gggtcgtcag
     9661 cttggggcga aatacccgaa cctgcatctg gcagggaaaa cagggactac caacaataac
     9721 gtagatactt ggtttgcagg cattgacggc agcacggtga ccatcacctg ggcaggccgt
     9781 gataacaacc agccgaccaa actgtatgga gccagcgggg caatgtcgat ttatcagcgt
     9841 tatctggcta accagacgcc aacgccgctg aatcttgttc cgccagaaga tattgcagat
     9901 atgggcgtgg actacgacgg caactttgtt tgcagcggtg gcatgcgtgt cttgccggtc
     9961 tggaccagcg atccgcaatc gctgtgccag cagagcgaga tgcagcagca gccgtcaggc
    10021 aatccgtttg atcagtcttc tcagccgcaa cagcaacctg ctcagcaaga gcagaaagac
    10081 agcgacggtg tagccggttg gatcaaggat atgtttggta gtaattaaca tctaagcgtg
    10141 aaataccgga tggcgagttg ccatccggta aaataacatc ccatctaaga tattaaccct
    10201 ttcttttcat ctggttgttt attaaccctt caggaacgct cagattgcgt accgcttgcg
    10261 aacccgccag cgtttcgaat attatcttat ctttataata atcattctcg tttacgttat
    10321 cattcacttt acatcagaga tataccaatg gcgcgttcca aaactgctca gccaaaacac
    10381 tcactgcgta aaatcgcagt tgtagtagcc acagcggtta gcggcatgtc tgtttatgca
    10441 caggcagcgg ttgaaccgaa agaagacact atcaccgtta ccgctgcacc tgcgccgcaa
    10501 gaaagcgcat gggggcctgc tgcaactatt gcggcgaagc actctgctac tgcgactaaa
    10561 acggatacac caattgaaaa aacgccgcag tctatttcgg ttgtgacaaa tgaagagatg
    10621 cagatgcatc aatttcagtc tgtaaaagaa gcattaggtt atacacctgg cgttacggtt
    10681 agcagtcgtg gtgcttctaa tacatatgat tttgttatca ttcgtggttt ctcatctgtt
    10741 ggtctgaatc aaaataatta ccttgatgga ctaaaacttc agggcaactt ttataacgat
    10801 gctgtgattg atccttacat gctcgagagg gttgaactga tgcgtgggcc gacgtctgtt
    10861 ctctatggaa aaagtaatcc tggtgggatt atttctatgg tgagtaagcg tccgaccact
    10921 gaacccctga aagaaattca gtttaaaatg gggacggata atctgtttca gaccggattc
    10981 gatttcagtg atgcactgga tgataacggt gagttctctt accgtttgac gggcctcgct
    11041 cgctcaacaa acgaacagca gaaaaactct gaatctcagc gttataccat tgctccatca
    11101 ttctcatggc gtccagacga caaaaccaat tttactttcc tgtcctattt ccagaatgaa
    11161 ccagaaacgg gttattacgg ttggttgccg aaagagggga ccgttgagcc attgcctaat
    11221 ggtaagcgtc taccgactga cttcaatgaa ggtgcatcga ataatacata ctcccgtaac
    11281 cagaaaatgg tgggatatag ttttgaacat ggttttaatg acaccttcac cgtgcgtcag
    11341 aatctgcgtt tcagtgaaat gaaaacctca cagaaaagtg tttatggcac agggattgct
    11401 ggtgatggtc ataccctaaa ccgcgggaca gtggtggata atgagcgtct acaaaacttt
    11461 agcgttgata cccaacttga aagtaagttt gctacaggtg acgtcgagca tactttgctg
    11521 acaggggtag acttcatgcg gatgcgtaat gatatcaatg ccagctttgg atccgcacca
    11581 tccatcgatc tttataacaa atatcaccct gaatactttg catttggtag cgcagagcca
    11641 taccagatga atgaaagcaa acaaacaggt atttatgttc aggatcaggc ggaatggaat
    11701 aaatgggtat tcactctggg gggacgctac gattggtcta agcaagcgac cactgttcgt
    11761 gaaaactctt atacgccgac tgaaggttat attgagcgca atgatcatca gttcacctgg
    11821 cgcggtggtg taaattactt attcgataat ggtatttcac cttactttag ctatagccag
    11881 tcctttgaac cgagtgcttt cgatctgtgg agcaacccac gcatttccta taagccatcg
    11941 aaaggtgaac agtatgaagc tggcgtaaaa tatgttccga atgatatgcc ggtcgttgtt
    12001 acgggcgcag tctatcaatt gacgaaaaca aataacctga cagcagaccc aacaaaccca
    12061 ttagcgcaag tcccagcagg tgagattcgc gctcgtggtg tggaacttga agcaaaagcg
    12121 gcgttaaatg ccaatattaa cttgacggct tcttatacct acaccgatgc ggaatacacc
    12181 aaagacacca atctcaaagg taaaactcca gaacaagtac cggagcatat ggcatctctc
    12241 tggggggatt ataccttcaa tgaagggccg ctttctggtt taacattggg aacaggtggt
    12301 cgttttattg gttccagcta tggtgatccg gcaaacactt ttaaagtggg tagcgcagct
    12361 gtaatggatg ctgttgtaaa atatgatctg gcacgctttg gtatggcggg atccagcctt
    12421 gctgtgaacg tcaacaattt gctcgatcgt gagtatgttg ccagttgctt ccagacttat
    12481 ggctgcttct ggggcgcaga acgtcaggtc gttgcaaccg caaccttccg tttctaattt
    12541 ctcttttggg gcacggattt ccgtgcccat ttcacaagtt ggctgttatg caggaataca
    12601 cgaatcattc cgataccact tttgcactgc gtaatatctc ctttcgtgtg cccgggcgca
    12661 cgcttttgca tccgctgtcg ttaacctttc ctgccgggaa agtgaccggt ctgattggtc
    12721 acaacggttc tagtaaatcc actctgctca aaatgcttgg ccgtcatcag cctccgtcag
    12781 aaggggagat tcttcttgat gcccagccgc tggaaagctg gagcagcaaa gcgtttgccc
    12841 gcaaagtggc ttatttgccg cagcagcttc ctccggcaga agggatgacc gtgcgtgaac
    12901 tggtggcgat tggtcgttac ccgtggcatg gcgcgctggg gcgctttggg gcggcagatc
    12961 gcgaaaaagt cgaggaagct atctcgctgg ttggcttaaa accgctggcg catcggctgg
    13021 ttgatagtct ctctggcggc gaacgtcagc gggcgtggat cgccatgctg gtggcgcagg
    13081 atagccgttg tctgttgctc gacgaaccga cctcggcgct ggatatcgcc caccaggttg
    13141 atgtgctggc gctggtgcac cgtttaagtc aggagcgtgg cctgacggtc attgccgtgt
    13201 tgcacgatat caatatggcg gcacgctact gtgattatct ggtcgccctg cgcggtggtg
    13261 aaatgattgc tcagggaacg cctgcggaaa ttatgcgcgg cgataccctc gaaatgattt
    13321 atggcatccc gatgggtatt ttgccgcatc cggcgggtgc tgcacctgtg agttttgttt
    13381 attgatgagc ggcttacctc ttatttcgcg ccgtcgactg ttaacggcga tggcgctttc
    13441 tccgttgtta tggcagatga ataccgccca cgcggcggct attgatccca atcgtattgt
    13501 ggcgctggag tggttgccgg tggaattact gctggcgctc ggcatcgtgc cttacggcgt
    13561 agcggatacc atcaactatc gcctgtgggt cagcgaacca ccattgccgg actcagtgat
    13621 cgacgtcggt ttgcgcacag aacctaacct tgaactgctg accgaaatga aaccgtcgtt
    13681 tatggtctgg tcggcaggat atggcccttc agcagaaatg ctggcacgta ttgcgccggg
    13741 gcgtggattt aacttcagcg acggcaaaca gccgctggcg atggcgcgta aatcactgac
    13801 ggaaatggca gatttactta acctgcaaag cgcagcagaa acgcatttag cgcaatatga
    13861 agactttatc cgcagcatga aaccccgctt tgtgaagcgt ggtgcacgcc cgttattgct
    13921 gacgacgctt atcgatccgc gccatatgtt ggtcttcggt ccaaacagct tgttccagga
    13981 aattcttgat gagtacggca tcccaaatgc ctggcaaggg gaaaccaact tctggggcag
    14041 taccgccgtc agtatcgatc gtctggcagc gtataaagac gttgatgtgc tctgttttga
    14101 tcacgacaac agcaaagaca tggatgcgct aatggcaacc ccgctgtggc aggccatgcc
    14161 gtttgtccgc gccggacgct ttcagcgcgt acccgcagtc tggttttatg gtgcgacgct
    14221 ctcggcaatg cactttgtgc gcgttctgga taacgccatc ggaggtaaag cgtgagtaaa
    14281 cgaattgcgc ttttcccggc gttattgctg gcgctgctgg tgattgtcgc tacggcgctg
    14341 acctggatga acttttcgca ggcgctgccg cgtagccagt gggcgcaggc cgcctggtcg
    14401 ccggatattg acgtcatcga gcagatgatt tttcactaca gcttgctgcc gcgtctggcg
    14461 atttcgctgc tggtgggcgc aggcctgggg ctggtgggcg tgctgtttca gcaagtgctg
    14521 cgtaatccgc tggcggaacc gacgacactt ggcgttgcta caggcgcgca actggggatt
    14581 accgtcacta cgctctgggc gatcccaggg gcgatggcga gtcagtttgc tgcgctggcg
    14641 ggcgcttgcg ttgttggctt aatcgtcttt ggcgtcgcgt gggggaaacg gctttcgccg
    14701 gtaacgctga tcctcgcggg gctggtagtg agcctttatt gtggggcaat caatcagtta
    14761 ctggttatct tccaccatga ccaactgcaa agcatgttcc tgtggagcac cggaacgctg
    14821 acgcaaaccg actggggcgg tgttgagcgt ttatggccgc agttgctggg cggtgtgatg
    14881 ctgacgttgc tgctacttcg cccgttaacc ctgatggggc ttgatgatgg cgtggcgcgc
    14941 aatctcgggc tggccttgtc gctcgcgcgt ctggcggcgc tgtcgctggc gattgtcatc
    15001 agtgcgctgc tggtgaacgc ggtggggatt atcggcttta tcgggttgtt tgcgccgttg
    15061 ctggcaaaaa tgctgggggc gcggcgtctg ctgccgcgac tgatgctggc gtcgttgatt
    15121 ggtgcgctga tcctctggct ttccgatcaa atcatcctct ggctgactcg cgtgtggatg
    15181 gaagtgtcca ccggttcggt cactgcgttg atcggtgcgc cgctgctact gtggctgttg
    15241 ccgcgtttac gcagcattag cgcgccggat atgaaggtca acgatcgtgt cgcggctgaa
    15301 cgccaacatg tgctggcgtt tgccctcgcg ggcggcgtgc tgctgttgat ggctgtggtg
    15361 gtggcgctgt cgtttggtcg tgatgcgcac ggctggatgt gggcgagcgg ggcgttgctc
    15421 gatgatttaa tgccctggcg ctggccgcga attatggcgg cgctatttgc gggcgtcatg
    15481 ctggcggtgg cgggctgtat tattcagcga ctgaccggca acccgatggc aagcccggaa
    15541 gtgctgggga ttagctccgg cgcagcgttt ggcgtggtac tgatgctgtt tctggtaccg
    15601 ggtaatgctt ttggctggct gttacccgcg ggaagtatcg gtgccgcggt gacgctgttg
    15661 attattatga tcgccgccgg gcgcggtgga ttttccccac accgcatgtt actggcgggg
    15721 atggcgttaa gtaccgcatt caccatgctt ttaatgatgt tgcaggcgag tggtgacccg
    15781 cgaatggcgc aagtgctcac ctggatctct ggttcgacct acaacgccac cgatgcgcag
    15841 gtctggcgta ccggaattgt gatggtgatt ttgctggcga ttaccccgct gtaccgccgc
    15901 tggctgacca ttttacctct gggcggtgat accgcacgag ctgtgggaat ggcgctgacg
    15961 ccgacgcgaa ttgcgctgct gctgttagcg gcttgcctga cggcgacctc gacgatgact
    16021 attggaccgt tgagttttgt tggtttaatg gcaccgcata ttgcgcggat gatgggtttt
    16081 cgacggacga tgccacacat cgtaatttcg gcgctggtgg gtggtttact gctggtattc
    16141 gctgactggt gtgggcggat ggtgctgttt ccattccaga tcccggcggg gctgctgtcg
    16201 acctttatcg gcgcgccata ttttatctat ttgttgagaa agcagagccg ttaattgagt
    16261 gcctcgcccg gtgggcgagg caaaattatc acaacttcgc aaatacccga cgtgcagcat
    16321 cgatggtgtt attgatatct tccatgctgt gcgccacaga cataaagccc gcttcaaacg
    16381 ctgacggtgc caggtaaaca ccttcgtcca gcatcatatg gaagaaacgc ttaaagcgtt
    16441 ccacgtcaca ggccatcaca tcctgatagc acgtcacgga ctcggcgtcg gtaaagaaaa
    16501 taccgaacat gccgccaacg tggttaacga ccagcggagt tccggcttct tctgccgctt
    16561 ccagcagacc ttctgccaga cgtgttgtca gctcatccag cgtttcgtga acgcccggct
    16621 gcgcgacttc attcagacag gcgaaacccg ctgccatcgc aatcgggtta ccggaaagcg
    16681 tacccgcctg atagaccgga cccgtcgggg ccagcgcatc cattacatca cgacgaccac
    16741 cgaatgcgcc taccggcatt ccaccgccga tgattttgcc gaggcaggtt aaatctggca
    16801 ctacgccgta ataatcctgt gcgccagcca gtgccacgcg gaagccggtc atcacttcat
    16861 cgatgatcag caacgcgcca aattcgtcgc acagcgcacg cagacctggc aggaactctg
    16921 gctgcggcgg aacacagttc atattgcctg ccaccggctc gacgataata caggcaatct
    16981 cttgcgggta ttgctcaaac gcggcgcgta cagaagccag atcgttataa gtacaggtta
    17041 aggtatgttt ggcgaaatct gccggaacac ccggcgagtt tggctggcct aacgtgagtg
    17101 cgccagaacc ggctttcacc agcaggcagt cagcgtgacc gtggtaacaa ccttcaaatt
    17161 taatgatttt gtcgcgaccg gtaaaaccac gggccaggcg gatggcgctc atggtcgctt
    17221 cagtgccgga gttcaccatg cgcaccatat ccatggtcgg gaccagttcg gtcaccagtt
    17281 gcgccatttt cacttccatt tcggtcggtg caccaaagct taaaccacgc tcggcggctt
    17341 caatcacggc attgcggata gccggatggt tatggcccag caccatcggc ccccaggaac
    17401 cgacataatc gatataggct ttgccatcaa catcgtacag ataagcgccg tccgcttttt
    17461 cgataaacag tggagtgccg cccacgccag taaaggcgcg aacaggggag ttcacaccgc
    17521 cagggagcag ctcgcgcgct gcgctgtaaa gattttcaga cttactcatg gagggttcct
    17581 gattcgtaga aaaagtgaat ggctgctatt ctatgttatt cataacaagt taaatactcg
    17641 tcaaacatca ggctgcttgt actggtgcaa tcctgatttc ttagagtata aaagttttgt
    17701 gcatttgaaa cattacgctt tgcaaaggat tttcatggaa cgtgcgagta aaatgccgtc
    17761 atcttatttg tatgaccaat aagtgatcat tggatgaaaa ctgatactcc ctctttagaa
    17821 acaccgcagg ccgcgcgcct gcgacgcaga caactgattc gccaacttct tgagcgcgat
    17881 aaaaccccgt tagccatttt gtttatggcg gcagtcgtcg gcacgcttgt cgggctggca
    17941 gcggttgctt ttgacaaagg tgtcgcctgg ttgcagaacc aacgtatgga ggcgctggta
    18001 catactgctg ataattatcc gcttctgtta accgtcgctt ttctctgttc ggcggtgctg
    18061 gcgatgttcg gctacttttt ggtgcgcaaa tacgcgccgg aagcaggtgg ttcggggatc
    18121 ccggaaattg aaggggcgct ggaagatcaa cgtcccgttc gctggtggcg tgtgttgccg
    18181 gtgaaattct ttggcgggct ggggacactc ggcggaggca tggtgctggg gcgcgaaggg
    18241 ccaaccgtgc agatcggcgg caatatcggt cgcatggtac tcgatatttt ccgcctgaaa
    18301 ggtgatgaag ctcgccatac gctgctggca accggtgctg ctgcggggct ggctgcggcc
    18361 tttaacgcgc cgctggcggg tattttgttt attatcgaag agatgcgtcc gcagtttcgc
    18421 tatacgttaa tttcgattaa agcggtattt attggcgtca ttatgtcgac cattatgtac
    18481 cggattttta atcatgaagt tgcgttgatt gacgtcggta aactttctga tgcgccgctt
    18541 aatacgctgt ggctttatct gatcctcggt attatttttg gcattttcgg ccctattttt
    18601 aataaatggg tgctggggat gcaggatttg ctgcaccgtg tgcacggcgg caatattacc
    18661 aaatgggtgc taatgggcgg tgcgattggc ggtctgtgtg gattgctggg gtttgtggca
    18721 ccagcaacgt cgggcggcgg ttttaacctg attcctatcg ctaccgcagg gaatttcagc
    18781 atgggaatgc tggtgtttat cttcgtcgcg cgggtcatta ccaccttact gtgcttctct
    18841 tccggcgcgc cgggcggtat ttttgccccg atgctggcgc tgggtactgt gctgggaacc
    18901 gctttcggaa tggttgtcgt tgagctgttt ccgcaatatc accttgaggc ggggactttt
    18961 gctattgccg gaatgggggc gttactggcg gcatctattc gcgcgccgtt aacggggatc
    19021 attttggttc tggagatgac cgataactac cagctcattt tgccaatgat tattaccggt
    19081 cttggcgcaa cactattagc gcaatttacc ggcgggaaac cgctatattc ggagattctt
    19141 gcgcgcacgc tggcaaaaca ggaagctgag caactggcgc gaagcaaggc cgcatcagcc
    19201 agcgagaata cttgaacgaa ataccagggt attagataat ggcgattatt attgggttag
    19261 aatttgccca attgccgatg tcgtttggag caaaatatga gtgatgacgt agcactgccg
    19321 ctggagttta ccgacgcagc agccaacaaa gttaaaagcc tgatcgctga cgaagataac
    19381 ccgaatctga aattacgcgt gtatatcacc ggtggcggtt gcagcggctt ccagtatggt
    19441 ttcacctttg atgatcaggt gaacgaaggc gatatgacca tcgaaaaaca gggcgttggc
    19501 ctggtggttg atccgatgag cctgcaatat ctggtcggtg gttccgttga ctataccgaa
    19561 ggactggaag gttctcgttt catcgtgacc aacccgaacg cgaaaagcac ctgcggttgc
    19621 ggttcttcct ttagcatcta atctgttgtt catgtgccgg ataaactatc cggcacatct
    19681 tctctcttca acgcccattc tcatccagcg caaacgtcgg tagcttaaga tgccaacgaa
    19741 tagccgccag ccgaatcaat agcgtcacga ccatgcccat catactggct gtttccagtg
    19801 gtacggaaaa tgtgtaataa gccgtagcgt ggacaatacc gccgataata caggcagttg
    19861 cgtagatttc tgtacgtaaa atcatgggga tttcgcgggc cagtacatca cgaatgatcc
    19921 cgccgccaac gccagtaatg acgcccatac aaaccgcgat taacggaccg gcttccgcat
    19981 taaaggcttt attcacgcca atgccgacaa acaccgccag accaacggcg tccagcaccg
    20041 gcaacatcca ttttggtaag cgtcttggct ggcgcaccag cacgatggtc agcatgctgg
    20101 ttaccattgc aacgaccaga tcggtgggat ctttcaccca aaataccggg ccgtgatcca
    20161 gcgccatgtc gcgaattgtc ccgccgccta cagcggtaac cacgcccagt accagaacac
    20221 caaaagggtc catacgcaat tttccggcta acaaaacgcc ggagatggca aataccgctg
    20281 tgccgactat atccagccaa tagacgagca ttgttcaatc cccactgagc acctaaaaag
    20341 gtcaggcgct aatctacctg tgaaagcgca ttacagagct gttgtgcagc gaggataata
    20401 cgtgggcttg cacgtcaaac cagtcactcg tgagaggaat aacgggaatt ttgagctgtt
    20461 caccccagta ttgtttgatt ttaggaattt ggtccggtcc gcctgtaatg acaatcgcct
    20521 gtggcgagcg tgctaacacc tgttcgcggc taacttgcgg ccaaggaacc cggctgtctt
    20581 taaagatgtt ttctccgcca caaacttcga gtacctggtt ttgaatcgac tcttttccac
    20641 tggtaaatgg cggattaatg ccgaattgca gaaaaacacg ttttttaggt ttatcagcat
    20701 attgcgcttt caattgcgcg tactgatcca gcagggattg cgcggcttgt tcggccttat
    20761 ccggttgcgg actccagggg gccagttgac gtaacgcatt ggcaatttgt tcaatgcttg
    20821 tcgcatcgac ccacatcact tttattcccg gcgaagccag ctggtcaacc tgccgctcgg
    20881 cattacctcc acgccaggca atcaccagat cgggtttcag cgcgacaatg cgttccagat
    20941 tcatcccctg ccaggtggaa acctgctcaa tcttttgcgc ttgtggagga tagtcggaat
    21001 agctgctgac cccaaccggc gtgatcccgg cggcaaaggc aagttcagtg ttggcgggag
    21061 aaagcgtgat gacgcgcggt gccgcgttga gccacagtgg cgcgagaaaa gacagggcga
    21121 ccagcgccct gaacagtgac ttagccatgt gccagtttct gcaccagtga ctcaaccatc
    21181 aggctggact gtttagcggc aacagccagg aactcatcga agctaagatg agactgttga
    21241 tcggccacgt cggagatggc gcgtacgaca acaaacggga cgttgaaatt gtggcagaca
    21301 tgggcgattg ccgtcgcttc catctctaca gcaatggcct gtgggaagtt gtggcggatt
    21361 ttcgccagac caacagaacc gttgatgaaa gcgtcgccgc taacaatcag gccacgtaca
    21421 gcgttaagat tcagttcggc aatgcaggcc tcagcggcag cgatcagttt atcgtcagct
    21481 ttaaagcctg ccggacagcc tggtaactga ccgtattcat aaccaaatgc cgtgacatcc
    21541 gcgtcgtgat aacgtgcttc gtccgagaca acgatatcgc ccactttcaa cgttggtgcc
    21601 aggccaccgg cagaaccggt gttaataatc acatctggct tgccgtgttc caacagcaaa
    21661 gtggcaccca gcgccgcagc gactttaccg atgcccgatt tcagaagcgc aacctcggtt
    21721 ccattcagtt ggccggtata gatttcgcaa ccgccgagac tgatagtttg acgattttcg
    21781 attttgtcac gcagcagcgt aacttcttct tccattgcac caatgatgcc gattttcata
    21841 gatttactcg cgataagccc gatttgaagg catagtttac catgcgctta cggggaagcg
    21901 tatttctcac gcgggagagg acatggcaca gattgatttc cgaaaaaaaa taaactggca
    21961 tcgtcgttac cgttcaccgc agggcgttaa aaccgaacat gagatcctgc ggatcttcga
    22021 gagcgatcgc gggcgtatca tcaactctcc ggcaattcgt cgtctgcaac aaaagaccca
    22081 ggtttttcca ctggagcgca atgccgccgt gcgcacgcgt cttacccact cgatggaagt
    22141 ccagcaggtg gggcgctaca tcgccaaaga aattctaagc cgtatgaaag agcttaaatt
    22201 actggaagca tacggcctgg atgaactgac cggccccttt gaaagcattg ttgagatgtc
    22261 atgcctgatg cacgatatcg gcaatccgcc gtttggtcat tttggcgaag cggcgataaa
    22321 tgattggttt cgccagcgtt tgcacccgga agatgccgaa agccagcctc tgactgacga
    22381 tcgctgcagc gtggcggcac tacgtttacg ggacggggaa gaaccgctta acgagctgcg
    22441 gcgcaagatt cgtcaggact tatgtcattt tgaggggaat gcacaaggca ttcgtctggt
    22501 gcatacattg atgaggatga atctcacctg ggcacaggtt ggcggtattt taaaatatac
    22561 ccgtccggcg tggtggcgtg gcgaaacgcc tgagacacat cactatttaa tgaaaaagcc
    22621 gggttattat ctttctgaag aagcctatat tgcccggttg cgtaaagaac ttaatttggc
    22681 gctttacagt cgttttccat taacgtggat tatggaagca gccgacgaca tctcctattg
    22741 tgtggcagac cttgaagatg cggtagagaa aagaatattt accgttgagc agctttatca
    22801 tcatttgcac gaagcgtggg gccagcatga gaaaggttcg ctcttttcgc tggtggttga
    22861 aaatgcctgg gaaaaatcac gctcaaatag tttaagccgc agtacggaag atcagttttt
    22921 tatgtattta cgggtaaaca ccctaaataa actggtaccc tatgcggcac aacgatttat
    22981 tgataatctg cctgcgattt tcgccggaac gtttaatcat gcattattgg aagatgccag
    23041 cgaatgcagc gatcttctta agctatataa aaatgtcgct gtaaaacatg tgtttagcca
    23101 tccagatgtc gagcagcttg aattgcaggg ctatcgggtc attagcggat tattagagat
    23161 ttatcgtcct ttattaagcc tgtcgttatc cgactttact gaactggtag aaaaagaacg
    23221 ggtgaaacgt ttccctattg aatcgcgctt attccacaaa ctctcgacgc gccatcggct
    23281 ggcctatgtc gaggctgtca gtaaattacc gtcagattct cctgagtttc cgctatggga
    23341 atattattac cgttaccgcc tgctgcagga ttatatcagc ggtatgaccg acctctatgc
    23401 gtgggatgaa taccgacgtc tgatggccgt agaacaataa ccaggctttt gtaaagacga
    23461 acaataaatt tttacctttt gcagaaactt tagttcggaa cttcgcgcta taaaacgaat
    23521 ctgaagaaca cagaaatttt gcgttatctg ttaatcgaga ctgaaataca tgaaaaaaac
    23581 cacattagca ctgagtgcac tggctctgag tttaggtttg gcgttatctc cgctctctgc
    23641 aacggcggct gagacttctt cagcaacgac agcccagcag atgccaagcc ttgcaccgat
    23701 gctcgaaaag gtgatgcctt cagtggtcag cattaacgta gaaggtagca caaccgttaa
    23761 tacgccgcgt atgccgcgta atttccagca gttcttcggt gatgattctc cgttctgcca
    23821 ggaaggttct ccgttccaga gctctccgtt ctgccagggt ggccagggcg gtaatggtgg
    23881 cggccagcaa cagaaattca tggcgctggg ttccggcgtc atcattgatg ccgataaagg
    23941 ctatgtcgtc accaacaacc acgttgttga taacgcgacg gtgattaaag tccagctgag
    24001 cgatggccgt aagttcgacg cgaagatggt cggcaaagat ccgcgctctg atatcgcgct
    24061 gatccaaatc cagaacccga aaaacctgac cgcaattaag atggcggatt ctgatgcact
    24121 gcgcgtgggt gattacaccg tagcgattgg taacccgttt ggtctgggcg agacggtaac
    24181 ttccgggatt gtctctgcgc tggggcgtag cggcctgaat gctgaaaact acgaaaactt
    24241 catccagacc gatgcagcga tcaaccgtgg taactccggt ggtgcgctgg ttaacctgaa
    24301 cggcgaactg atcggtatca acaccgcgat cctcgcaccg gacggcggca acatcggtat
    24361 cggttttgca atcccgagca acatggtgaa aaacctgacc tcgcagatgg tggaatacgg
    24421 ccaggtgaaa cgcggtgagc tgggtattat ggggactgag ctgaactccg aactggcgaa
    24481 agcgatgaaa gttgacgccc agcgcggtgc tttcgtaagc caggttctgc ctaattcctc
    24541 cgctgcaaaa gcgggcatta aagcgggtga tgtgatcacc tcactgaacg gtaagccgat
    24601 cagcagcttt gccgcactgc gtgctcaggt gggtactatg ccggtgggca gcaaactgac
    24661 cctgggctta ctgcgcgacg gtaagcaggt taacgtgaac ctggaactgc agcagagcag
    24721 ccagaatcag gttgattcca gctccatctt caacggcatt gaaggcgctg agatgagcaa
    24781 caaaggcaaa gatcagggcg tggtagtgaa caacgtgaaa acgggcactc cggctgcgca
    24841 gatcggcctg aagaaaggtg atgtgattat tggcgcgaac cagcaggcag tgaaaaacat
    24901 cgctgaactg cgtaaagttc tcgacagcaa accgtctgtg ctggcactga acattcagcg
    24961 cggcgacagc accatctacc tgttaatgca gtaatctcac tcaacccctt cctgaaaacg
    25021 ggaaggggtt ctccttacaa tctgtgaact tcaccacaac tccatacatc ttcatcatcc
    25081 tttaggcatt tgcacaatgc cgtacgttac gtacttcctt atgctaagcc gtgcataacg
    25141 gaggacttat ggctggctgg catcttgata ccaaaatggc gcaggatatc gtggcacgta
    25201 ccatgcgcat catcgatacc aatatcaacg taatggatgc ccgtgggcga attatcggca
    25261 gcggcgatcg tgagcgtatt ggtgaattgc acgaaggtgc attgctggta ctttcacagg
    25321 gacgagtcgt cgatatcgat gacgcggtgg cacgtcatct gcacggtgtg cggcaaggga
    25381 ttaatctacc gttacggctg gaaggtgaaa ttgtcggcgt aattggcctg acaggtgaac
    25441 cagagaatct gcgtaaatat ggcgaactgg tctgcatgac ggctgaaatg atgctggaac
    25501 agtcgcggtt gatgcacttg ttggcgcagg atagccgttt gcgggaggaa ctggtgatga
    25561 acctgattca ggcagaggag aatactcccg cacttactga atgggcgcaa cggctgggga
    25621 tcgatctcaa tcaaccgcga gtggtggcta ttgttgaggt cgacagcggt cagcttggtg
    25681 tggacagcgc aatggcggag ttacaacaac tgcaaaacgc gctgactacg cccgagcgta
    25741 ataatctggt ggcgattgtc tcgctaaccg aaatggtggt gttgaaaccg gcgttgaact
    25801 cttttgggcg ctgggatgca gaagatcatc gtaagcgagt tgaacaactg attacccgca
    25861 tgaaagagta cggccggctg cgttttcgcg tttcactggg caactatttt accggtcctg
    25921 gcagtattgc ccgatcctat cgtacggcga aaacgacgat ggtggtgggt aaacagcgga
    25981 tgccagaaag tcgctgctat ttttatcagg atctgatgtt acctgtgtta ctcgacagtt
    26041 tgcgtggcga ctggcaggcc aacgaactgg cgcgaccgct ggcgcggctg aaagcgatgg
    26101 acaataacgg cttgctgcga cgaacgctgg cggcgtggtt tcgtcacaat gtgcaaccgc
    26161 tggcaacgtc aaaggcgttg tttatttatc gtaataccct agagtatcgg cttaatcgta
    26221 tatcggaact gaccgggctt gatttgggca attttgatga caggttgctg ctgtatgtgg
    26281 cgttacaact ggatgaagag cggtaggtta tgcgttaagg ttgaggcggc gctgtctcat
    26341 ttccctcagc ccgaagggga gaggggaatg cccgaattaa ccgtcaattc gggattattt
    26401 acgcgttagt ttttccagat cggcttcaat ctcgctgatc ttattggtta cgactgactc
    26461 caggtgacgt aagtcgtcga ggatcttacg cttaagatca acttcgctgc ggtcacgctg
    26521 gcagatttga tcaagctcat caatgatata ccgtagattc gggctgattt cctggacttc
    26581 tttataaccc tgacccacac catcagcgac gaccgtttta cgctgacgcg gatatttaaa
    26641 cttaacgctc ttggcgaaaa actcgccttt gtctttctgg aaatagattt tcagaatatc
    26701 gttgttggct tcctgccgga ggctgtaacg atcaatttct tcaggattgg taatacccag
    26761 acttttcaga ttgtcgtaca tagcgttacc tcaaaatgag tcagtaaatt gtgcttattt
    26821 tagcatttgg cctagcccgc ccggctcgac tgtgatcgca gaaagccttt ttcttccaga
    26881 cttctatcat ggcgtaaaaa tcaaaaaatt acctgcttta ttgtggtgat aaaattcacg
    26941 ttctacacca gttccagcat cgttacttcc gggcggcagt tcagacgcaa accatacaaa
    27001 ctgcccacgc cacgggttgt gtagatgtgt cgttcgccaa aggcatttaa tccggcgacg
    27061 taacgtttat cttcgacagg ggcaaaaggt tctccaacca acggtacgcg cagttgcccg
    27121 ccgtgagtat ggccgcacag catcagatcc cagggttcat cacgcatgac ttctttgctg
    27181 tcgggattat gtgccagcat cagtcttggc agatttgctt cgctggcggg aggtggtttg
    27241 cattgtccgg cccataagtc accggtgcct accagttcga attgcctgtt cggcgtggcg
    27301 atcaccgtag cctggttaaa caacaccgtg atgcccactg atttcaacgt ctcgccaatt
    27361 aagtgatttt tttctgtacc aacagggcga tcgtggttgc cgaagcaggc aaacgtcggc
    27421 gcacattcgg caagggggga gaggacatca ctaaacgccg aaaaattcag cggcatatca
    27481 aataatacgt aatcgccgcc cagtaatatc aaatcgggct tttgttctat gccaagagca
    27541 atcgcgtcag aaatcaggct taaaggaaca aaacgagagt aatggagatc ggccagaaaa
    27601 agaattttga atggtgctgc gttgtcttta aaaaaggcga ggcggtgacg gattaattca
    27661 aaccagcctg gctcccagta atgcatataa ccgaaacctg agctcgtggc tatcgtcgcg
    27721 gcagtagcct gcaacaatcg gcggcgtgaa atcatcgctc atccctgcaa aaaaaatcgg
    27781 gcagcgtcgt gctgcccgtg tgcatacttt tagtcgatgg tacgcagcag ttcgttaatg
    27841 ccgactttgc cgcgagtttt cgcgtcaact ttcttaacga taaccgcaca gtagaggctg
    27901 tatttgccat cttttgacgg cagattacct gaaacaacca cagaccccgc cggaacgtga
    27961 ccgtagtgga tttcgccggt ttcacggtcg taaatacggg tgctctgacc aatgtatacg
    28021 cccatggaaa tgacggaacc ttcttcgaca atcacccctt caactacttc agagcgtgcg
    28081 ccaatgaagc agttatcttc aatgatggtt gggttagcct gcagcggttc cagcacgccg
    28141 ccgatgccca cgccaccgga aaggtggacg tttttaccaa tctgcgcaca agaaccgacg
    28201 gtcgcccagg tatcaaccat ggtgccttca tcaacatatg cgccgatgtt gacgtaagac
    28261 ggcatcagca cggtgttacg ggcaataaac gcaccctgac gtaccgccgc tggtggcaca
    28321 acgcggaagc cttctttctg gaaacgtgct tcgtcgtagt cggcgaattt cattggcact
    28381 ttgtcgaagt agcggctttc tgccccttcg atcacctgat tatcattaat acggaaagag
    28441 agcagcaccg cttttttcaa ccactgatgc gtcacccact gaccgtcaat tttttccgct
    28501 acacgcagtg caccggaatc cagcagggcg atcacctgat ttaccgcttc gcgggtaacg
    28561 gtgtctgcgt tggttggcgt gatctcggca cggcgttcaa aagcggtttc aataatgttc
    28621 tgtaactgct gcattgttaa actcttttca tatcagtaaa cacatcaccc tttatcgttt
    28681 ggattgaggg cctctgtcaa ccgctgatgc acttcctgct gcaactcgtt attaagcgca
    28741 cgccggtcag cggtggcaat tatgaataaa tcttctactc gctcgccaat ggttgtaatt
    28801 cgggcaccat gaagcgaaat tcccagatcg gcaaaaattt tcccgactct cgccagcagt
    28861 cctggctggt cgagcgcgat aagctcaagg aacgatttgc ggtcggtatg ggtcggcaaa
    28921 aacgttactt cggtttcaac agtaaaatgg cgtaatttgg cgggttggcg acggggctgc
    28981 ggtggctgcc agctactttg cgtcagtact tgctccagac caaaccgaat aacctcatga
    29041 cgatctgcgg acagcgggct gccatcgggt tccagcacga taaaggtatc catcgccata
    29101 ccgtcgcgag tggtgaaaat ttgtgcgtcg tgaacactta aattgcggcg gtctaattcg
    29161 gcacagacgg cggcaaacag ataggggcgg tccgggctcc agataaaaat ctcggtgcct
    29221 ccacgcgtag cctgcgggct aagcaatacc agtggtttgc ttaaatcatg ctgtaataaa
    29281 tggcgggcat gccaggccag ttgatttggg ctatggcgga caaaatagtt agcacgacag
    29341 cgtgaccaaa tttggtgcag cgcctcttcg tcgatgttat ccatgcgcag tagtgccagt
    29401 gcctggagtt ggtgatggcg aacccgttcg cgcatatccg gcgtgttttg cattccgcgt
    29461 cgtagctgct tttcggtggc aaaatagagt tcgcgcaaca gactttgctt ccagctattc
    29521 cacagcgttt cgttggtggc gcaaatgtca gccactgtca ggcataccag atagcgcaga
    29581 cgattttccg tttgaacttc ttcggcaaac tgcttgatga cttcagggtc ctgaatatcg
    29641 cggcgttgtg ctgtcaccga catcagcaag tgctggcgaa ccagccaggc aaccagctgt
    29701 gtttcgcgtg agttcagccc gtggagttct gcaaaatgca ctacatcctg agcaccgaga
    29761 atggagtggt cgccgccgcg tcctttggcg atatcgtgaa acagcgcggc gatgaaaatc
    29821 agctcagttg acggcaggcg cggccagacg tccacacaca acggatggcg ctggcgcgtt
    29881 tcttcactgg caaaactctc cagtttcagc atcacgcgga tagtatgttc atccaccgtg
    29941 taggcgtgga acagatcaaa ctgcatctgc ccgacgatat gcgaccattg cggcatatac
    30001 gcgccgagca cgctatggcg atgcattggc aatagcccgc gccgcaccgc tccggggtga
    30061 cgcagaatgc tcaaaaacag ttttcgtgct tccggaatat tacacagcgg ttgttgcaga
    30121 tggcgacggg catggcgtaa ctggcgcagc gtggtggagt aaatgccggt gatcgcactg
    30181 ttgcgcacca tggtgtagaa catacgcaag atggcttccg gctggcgcat aaatagtgtt
    30241 tcatcacgca ggtcgattag cgtaccgcgt agctgaaact catcgtcgat tggacgtggt
    30301 ttttcgtcgg caggaagggc gaggatggct tcatcgaaca gttgcagcag catctggttg
    30361 agttcactga cgcggcgtgt aacgcggaag taatccttca tcatccgctc gaccggttcg
    30421 ttgccttcac cactgtaatt cagacgctga gcgacgctaa gttggcgatc gaataacagg
    30481 cgattatcgt aacggctgac gaccagatgc agggcaaagc gaatacgcca cagtatatgc
    30541 agacattcgt ttaattccgc ccgctccgct gaggttaaga agccaaaccc aaccatttca
    30601 tccagcgatg ttgcgccaaa atgacggcgg gccacccatt gcagagtgtg gatatcgcgc
    30661 aagccgccag ggctactttt gatgtctggt tcaagattgt agctggtgcc gtggtaacgc
    30721 tgatggcgct ggttctgttc ttcaactttc gccgcgtaga acttgtcgga aggccagaat
    30781 ccttcgctaa aaatatgttt ttgcagttcg aggaagagcg caacatcgcc aattaataag
    30841 cgggattcga ttaaattggt ggcgacggtt aaatccgata acccttccag catgcactct
    30901 tcaagcgtgc gcacactatg accgacttcc agctttacat cccagagcag cgttaacagc
    30961 tcgcccactt tttgcgcctg atcgtccggg agctttttac ggcttaaaat cagtaaatcg
    31021 atgtcagaaa gtggatgcag ctcgccacga ccgtagccac cgacggcgac caatgccagg
    31081 tcggcaatct ggctgaatcc cgcttcaatc cataatcgtt gcaggagctg gtcgataaac
    31141 tcggtgcgcg cctcaatcaa ctgttctgca gagatcccat tgtcaaaggc atcacccagc
    31201 caacgctgga aagtatcgat atgggctttt atcccaccga cggttaattc atcacggggc
    31261 cagacgcatg gattttgcgg ttgaccgggc agggtgggga gagcggtgtt tgcgtactgt
    31321 tctggaaggg tattcattgt gcgccactca taagattaaa ttatcgcatt aaaaaagccg
    31381 gcattcgccg gcttcatctt attcgtcgtg cgagattatc gccgggatgg tgtcatcctt
    31441 gcgtagcgtc agaatttcgc agccgttatc agtcaccaca atagtatgct catattgtgc
    31501 agacaagctg cgatctttgg tttttaccgt ccagccatct ttcatggtgc ggatctcttt
    31561 tttacccgcg ttgaccattg gctcgatggt gaacgtcatc ccaggtttca gtacgacgtt
    31621 ggtttcacgg gagtcatagt gcagcacctg cggttcttca tggaagccgc gaccaatacc
    31681 gtgtccgcaa tattcacgaa cgacggagaa gccttctgct tcgacaaact tctgaatcgc
    31741 cgcaccgatt tcgcgcaggt taatgcctgg tttgaccatg cgcagcgcca ggtacaggct
    31801 ttcttgcgtg atgcggcaca gacgttcgcc catgatggtc ggcttaccga cgataaacat
    31861 tttcgaggta tcgccgtgga aaccatcttt gattacggtg acatcaatgt taacgatatc
    31921 gccatctttc agcagcttcg catcgtccgg gataccgtgg cacaccactt cattaataga
    31981 gatgcaaacg gatttcggat agccgtgata gccgaggcag gcagaaaccg cgtgttgttc
    32041 attaacaatg taatcattac agatgcgatc cagctcgccg gtgctgacgc ccggtttaac
    32101 atacggttcg atcatctcca gcacttcggc agccagtcgg ccagcgacgc gcattttttc
    32161 gatatcttct ggggtcttga ttgagatagc cattaattct gtccatcagc gtcggtgata
    32221 ccgacaatat atatgtaagt gccgtcaatg gtatcacacc cgggcaaatt gagaatcatt
    32281 ctgaatttcg ccaaacgtgc cactgaacgt tttctataat agaaaattca acgtctgatg
    32341 ctgtacacag cgccaacaat tattggtgtc catgacgtat ttgtggtata aagcgcgccg
    32401 gacttccgat ccatttcgta tacacagact ggacggaagc gacaatctca ctttgtgtaa
    32461 caacacacac gtatcggcac atattccggg gtgccctttg gggtcggtaa tatgggatac
    32521 gtggaggcat aaccccaact tttatataga ggttttaatc atggcaactg tttccatgcg
    32581 cgacatgctc aaggctggtg ttcacttcgg tcaccagacc cgttactgga acccgaaaat
    32641 gaagccgttc atcttcggtg cgcgtaacaa agttcacatc atcaaccttg agaaaactgt
    32701 accgatgttc aacgaagctc tggctgaact gaacaagatt gcttctcgca aaggtaaaat
    32761 ccttttcgtt ggtactaaac gcgctgcaag cgaagcggtg aaagacgctg ctctgagctg
    32821 cgaccagttc ttcgtgaacc atcgctggct gggcggtatg ctgactaact ggaaaaccgt
    32881 tcgtcagtcc atcaaacgtc tgaaagacct ggaaactcag tctcaggacg gtactttcga
    32941 aaagctgacc aagaaagaag cgctgatgcg cactcgtgag ctggagaaac tggaaaacag
    33001 cctgggcggt atcaaagaca tgggcggtct gccggacgct ctgtttgtaa tcgatgctga
    33061 ccacgaacac attgctatca aagaagcaaa caacctgggt attccggtat ttgctatcgt
    33121 tgataccaac tctgatccgg acggtgttga cttcgttatc ccgggtaacg acgacgcaat
    33181 ccgtgctgtg accctgtacc tgggcgctgt agctgcaacc gtacgtgaag gccgttctca
    33241 ggatctggct tcccaggcgg aagaaagctt cgtagaagct gagtaataag gcttgataac
    33301 tcccccaaaa tagttcgagt tgcagaaagg cggcaagctc gagaattccc gggagcttac
    33361 atcagtaagt gaccgggatg agcgagcgaa gataacgcat ctgcggcgcg aaatatgaag
    33421 ggggagagcc cttatagacc aggtagtaca cgtttggtta gggggcctgc atatggcccc
    33481 ctttttcact tttatatctg tgcggtttaa tgccgggcag atcacatctc cgaggatttt
    33541 agaatggctg aaattaccgc atccctggta aaagagctgc gtgagcgtac tggcgcaggc
    33601 atgatggatt gcaaaaaagc actgactgaa gctaacggcg acatcgagct ggcaatcgaa
    33661 aacatgcgta agtccggtgc tattaaagca gcgaaaaaag caggcaacgt tgctgctgac
    33721 ggcgtgatca aaaccaaaat cgacggcaac tacggcatca ttctggaagt taactgccag
    33781 actgacttcg ttgcaaaaga cgctggtttc caggcgttcg cagacaaagt tctggacgca
    33841 gctgttgctg gcaaaatcac tgacgttgaa gttctgaaag cacagttcga agaagaacgt
    33901 gttgcgctgg tagcgaaaat tggtgaaaac atcaacattc gccgcgttgc tgcgctggaa
    33961 ggcgacgttc tgggttctta tcagcacggt gcgcgtatcg gcgttctggt tgctgctaaa
    34021 ggcgctgacg aagagctggt taaacacatc gctatgcacg ttgctgcaag caagccagaa
    34081 ttcatcaaac cagaagacgt atccgctgaa gtggtagaaa aagaatacca ggtacagctg
    34141 gatatagcga tgcagtctgg taagccgaaa gaaatcgcag agaaaatggt tgaaggccgc
    34201 atgaagaaat tcaccggcga agtttctctg accggtcagc cgttcgttat ggaaccaagc
    34261 aaaactgttg gtcagctgct gaaagagcat aacgctgaag tgactggctt catccgcttc
    34321 gaagtgggtg aaggcatcga gaaagttgag actgactttg cagcagaagt tgctgcgatg
    34381 tccaagcagt cttaattatc aaaaaggagc cgcctgaggg cggcttcttt ttgtgcccat
    34441 cttgtaaatt cagctaaccc ttgtggggct gcgctgaaaa gcgacgtaca atgtcgctag
    34501 tattaattca tttcaatcgt tgacagtctc aggaaagaaa catggctacc aatgcaaaac
    34561 ccgtctataa acgcattctg cttaagttga gtggcgaagc tctgcagggc actgaaggct
    34621 tcggtattga tgcaagcata ctggatcgta tggctcagga aatcaaagaa ctggttgaac
    34681 tgggtattca ggttggtgtg gtgattggtg ggggtaacct gttccgtggc gctggtctgg
    34741 cgaaagcggg tatgaaccgc gttgtgggcg accacatggg gatgctggcg accgtaatga
    34801 acggcctggc aatgcgtgat gcactgcacc gcgcctatgt gaacgctcgt ctgatgtccg
    34861 ctattccatt gaatggcgtg tgcgacagct acagctgggc agaagctatc agcctgttgc
    34921 gcaacaaccg tgtggtgatc ctctccgccg gtacaggtaa cccgttcttt accaccgact
    34981 cagcagcttg cctgcgtggt atcgaaattg aagccgatgt ggtgctgaaa gcaaccaaag
    35041 ttgacggcgt gtttaccgct gatccggcga aagatccaac cgcaaccatg tacgagcaac
    35101 tgacttacag cgaagtgctg gaaaaagagc tgaaagtcat ggacctggcg gccttcacgc
    35161 tggctcgtga ccataaatta ccgattcgtg ttttcaatat gaacaaaccg ggtgcgctgc
    35221 gccgtgtggt aatgggtgaa aaagaaggga ctttaatcac ggaataattc ccgtgatgga
    35281 taaataaggg taagattccg cgtaagtatc gcgggggcgt aagtctggtt ataaggcgtt
    35341 attgttgcag gcagtttggt cacggccagc gcgcagcaac cggagcgtac aaaagtacgt
    35401 gaggatggcg agcactgctc ggggccaaaa tggcaaataa aatagcctaa taatccagac
    35461 gattacccgt aatatgttta atcagggcta tacttagcac acttccactg tgtgtgactg
    35521 tctggtctga ctgagacaag ttttcaagga ttcgtaacgt gattagcgat atcagaaaag
    35581 atgctgaagt acgcatggac aaatgcgtag aagcgttcaa aacccaaatc agcaaaatac
    35641 gcacgggtcg tgcttctccc agcctgctgg atggcattgt cgtagaatat tacggcacgc
    35701 cgacgccgct gcgtcagctg gcaagcgtaa cggtagaaga ttcccgtact ctgaaaatca
    35761 acgtgtttga tcgttcaatg tctccggccg ttgaaaaagc gattatggcg tccgatcttg
    35821 gcctgaaccc gaactctgcg ggtagcgaca tccgtgttcc gctgccgccg ctgacggaag
    35881 aacgtcgtaa agatctgacc aaaatcgttc gtggtgaagc agaacaagcg cgtgttgcag
    35941 tacgtaacgt gcgtcgtgac gcgaacgaca aagtgaaagc actgttgaaa gataaagaga
    36001 tcagcgaaga cgacgatcgc cgttctcagg acgatgtaca gaaactgact gatgctgcaa
    36061 tcaagaaaat tgaagcggcg ctggcagaca aagaagcaga actgatgcag ttctgatttc
    36121 ttgaacgaca aaaacgccgc tcagtagatc cttgcggatc ggctggcggc gttttgcttt
    36181 ttattctgtc tcaactctgg atgtttcatg aagcaactca ccattctggg ctcgaccggc
    36241 tcgattggtt gcagcacgct ggacgtggtg cgccataatc ccgaacactt ccgcgtagtt
    36301 gcgctggtgg caggcaaaaa tgtcactcgc atggtagaac agtgcctgga attctctccc
    36361 cgctatgccg taatggacga tgaagcgagt gcgaaacttc ttaaaacgat gctacagcaa
    36421 cagggtagcc gcaccgaagt cttaagtggg caacaagccg cttgcgatat ggcagcgctt
    36481 gaggatgttg atcaggtgat ggcagccatt gttggcgctg ctgggctgtt acctacgctt
    36541 gctgcgatcc gcgcgggtaa aaccattttg ctggccaata aagaatcact ggttacctgc
    36601 ggacgtctgt ttatggacgc cgtaaagcag agcaaagcgc aattgttacc ggtcgatagc
    36661 gaacataacg ccatttttca gagtttaccg caacctatcc agcataatct gggatacgct
    36721 gaccttgagc aaaatggcgt ggtgtccatt ttacttaccg ggtctggtgg ccctttccgt
    36781 gagacgccat tgcgcgattt ggcaacaatg acgccggatc aagcctgccg tcatccgaac
    36841 tggtcgatgg ggcgtaaaat ttctgtcgat tcggctacca tgatgaacaa aggtctggaa
    36901 tacattgaag cgcgttggct atttaacgcc agcgccagcc agatggaagt gctgattcac
    36961 ccgcagtcag tgattcactc aatggtgcgc tatcaggacg gcagtgttct ggcgcagctg
    37021 ggggaaccgg atatgcgtac gccaattgcc cacaccatgg catggccgaa tcgcgtgaac
    37081 tctggcgtga agccgctcga tttttgcaaa ctaagtgcgt tgacatttgc cgcaccggat
    37141 tatgatcgtt atccatgcct gaaactggcg atggaggcgt tcgaacaagg tcaggcagcg
    37201 acgacagcat tgaatgccgc aaacgaaatc accgttgctg cttttcttgc gcaacaaatc
    37261 cgctttacgg atatcgctgc gttgaattta tcggtactgg aaaaaatgga tatgcgcgaa
    37321 ccacaatgtg tggacgatgt gttatctgtt gatgcgaacg cgcgtgaagt cgccagaaaa
    37381 gaggtgatgc gtctcgcaag ctgaggataa tccggctaca gagagtctcg ctatttgtta
    37441 gcgtagggct tcagtgatat agtctgcgcc atctgatcgt aagtagttgg ctttataagg
    37501 tcaggtatgc cgtggttttg cacggctttt tttgtatagg cttcagtatt cctgagtacc
    37561 gtaaaccctg tcagggaata aaaaacgcgt gatgttgtct gctactcaac cacttagcga
    37621 aaaattgcca gcgcatggct gccgtcatgt tgcgatcatt atggacggca atggtcgctg
    37681 ggcaaaaaag caagggaaga ttcgtgcctt tgggcataaa gccggggcaa aatccgtccg
    37741 ccgggctgtc tcttttgcgg ccaacaacgg tattgaggcg ttaacgctgt atgcctttag
    37801 tagtgaaaac tggaaccgac cagcgcagga agtcagtgcg ttaatggaac tgtttgtgtg
    37861 ggcgctcgat agcgaagtaa aaagtctgca ccgacataac gtgcgtctgc gtattattgg
    37921 cgataccagt cgctttaact cgcgtttgca agaatgtatt cgtaaatctg aagcgctaac
    37981 agccgggaat accggtctga cgctgaatat tgcggcgaac tacggtggac gttgggatat
    38041 agtccaggga gtcaggcaac tggctgaaaa ggtgcagcaa ggaaacctgc aaccagatca
    38101 gatagatgaa gagatgctaa accagcatgt ctgtatgcat gaactggccc ctgtagattt
    38161 agtaattagg actggggggg agcatcgcat tagtaacttt ttgctttggc aaattgccta
    38221 tgccgaactt tactttatag atgttctctg gcccgatttc gatgaacaag actttgaagg
    38281 ggcgttaaat gcctttgcta atcgagagcg tcgtttcggc ggcaccgagc ccggtgatga
    38341 aacagcctga tggggggcgc ttttgctgaa gtatcgcctg atatctgctt ttgtgttaat
    38401 acccgtcgtc atcgcggcgt tgtttctgtt gccgccggtg gggttcgcca ttgtaacgct
    38461 ggtggtctgc atgctggcag cgtgggaatg gggacagctt agcggtttta ccactcgttc
    38521 gcagcgagta tggttggcgg tgttatgcgg gttattgttg gcgctgatgc tttttctgtt
    38581 gccggaatat caccgaaata ttcatcaacc gctggttgaa atctcacttt gggcttcgct
    38641 gggttggtgg attgtctcgc tattgctggt gctgttttac ccaggttccg cagcaatctg
    38701 gcgtaactct aaaacattgc gccttatttt tggcgtgcta accattgttc ccttcttctg
    38761 gggcatgctg gcgttacggg cctggcacta tgacgagaat cattacagtg gcgcaatatg
    38821 gctgctctat gtcatgatcc tggtatgggg cgctgactcc ggcgcatata tgtttggcaa
    38881 attgtttggt aaacataagc tggcaccgaa ggtttctccg ggtaaaacct ggcaaggctt
    38941 tataggtgga ctcgctactg cagcggtaat ctcatggggt tatggcatgt gggcgaatct
    39001 cgacgtcgct cccgtcacct tactcatttg ctctattgtc gcagcgttag cctcagtgct
    39061 cggcgatctg accgagagta tgtttaagcg tgaagcagga attaaggaca gcggtcattt
    39121 aattccagga cacggtggta ttttagatcg tattgatagc ctgacggctg cggtaccggt
    39181 ctttgcttgc ttgttgttac tggtattcag gacgctttaa cggaaggtaa tatgctgagt
    39241 tttctctggg atttggcttc gttcatcgtt gcactgggtg tacttatcac tgtgcatgaa
    39301 tttggtcatt tctgggttgc ccggcgttgt ggtgttcgcg ttgagcgttt ctcaataggg
    39361 tttggtaagg cgctctggcg gcgaactgat aagctcggca ccgaatatgt tatcgccctg
    39421 atcccgctgg gcggttatgt caaaatgctg gatgagcgcg cagaaccggt cgttccggaa
    39481 ctccgccacc atgccttcaa taataaatct gttggccaac gagcggcgat tattgccgca
    39541 ggtccggttg caaacttcat ttttgctatc tttgcctact ggctggtttt tattattggt
    39601 gtgcctggcg tacgtccggt ggttggtgaa atagcagcca attcgatagc tgcggaagca
    39661 caaattgcac caggtacgga actaaaagcc gtagatggta tcgaaacgcc tgattgggat
    39721 gccgtgcgtt tgcagttggt cgataaaatt ggcgatgaaa gcaccaccat tacagtagcg
    39781 ccatttggca gcgaccaacg gcgggatgta aagctcgatt tacgtcactg ggcgtttgag
    39841 cctgataaag aagatccggt atcttcgctg gggattcgtc ctcgtgggct gcaaattgaa
    39901 cctgtactgg aaaatgtgca gccaaactcg gcggcaagca aggcaggttt gcaagcaggc
    39961 gacaggatcg ttaaagtcga tggtcagccc ttaacgcagt gggtgacctt tgtgatgctt
    40021 gtccgggata acccggggaa atccttagcg ttagaaatcg aaaggcaggg aagtctcttg
    40081 tctttgacat taatcccgga gagtaaaccg ggtaatggta aagcgattgg ttttgtcggt
    40141 attgagccga aagtcattcc tttgccagat gagtataaag ttgtacgcca gtatgggccg
    40201 ttcaacgcca tcgtcgaagc cacggacaaa acgtggcagc tgatgaagct gacggtcagt
    40261 atgctgggaa aattgatcac cggtgatgtg aaactgaaca acctcagtgg gccgatctct
    40321 atcgccaagg gggctgggat gacagcggaa ctcggggtag tttattacct gccgtttctt
    40381 gcgcttatta gcgtgaactt agggataatt aacctgtttc cgttgcccgt acttgacggg
    40441 gcgcatctgc tgttccttgc gatcgaaaag atcaagggcg gaccggtatc cgagcgggtt
    40501 caagactttt gttatcgcat tggctcgatt ctgctggtgc tgttaatggg gcttgcactt
    40561 ttcaatgatt tctctcggtt atgagagtta gttaggaaga acgcataata acgatggcga
    40621 tgaaaaagtt gctcatagcg tcgctgctgt ttagcagcgc caccgtatac ggtgctgaag
    40681 ggttcgtagt gaaagatatt catttcgaag gccttcagcg tgtcgccgtt ggtgcggccc
    40741 tcctcagtat gccggtgcgt acaggcgaca cggttaatga tgaagatatc agtaatacca
    40801 ttcgcgctct gtttgctacc ggcaactttg aggatgttcg cgtccttcgt gatggtgata
    40861 cccttctggt tcaggtaaaa gaacgtccga ccattgccag cattactttc tccggtaaca
    40921 aatcggtgaa agatgacatg ctgaagcaaa acctcgaggc ttctggtgtg cgtgtgggcg
    40981 aatccctcga tcgcaccacc attgccgata tcgagaaagg tctggaagac ttctactaca
    41041 gcgtcggtaa atatagcgcc agcgtaaaag ctgtcgtgac cccgctgccg cgcaaccgtg
    41101 ttgacctaaa actggtgttc caggaaggtg tgtcagctga aatccagcaa attaacattg
    41161 ttggtaacca tgctttcacc accgacgaac tgatctctca tttccaactg cgtgacgaag
    41221 tgccgtggtg gaacgtggta ggcgatcgta aataccagaa acagaaactg gcgggcgacc
    41281 ttgaaaccct gcgcagctac tatctggatc gcggttatgc ccgtttcaac atcgactcta
    41341 cccaggtcag tctgacgcca gataaaaaag gtatttacgt cacggtgaac attaccgaag
    41401 gcgatcagta caagctttct ggcgttgaag tgagcggcaa ccttgccggg cactccgctg
    41461 aaattgagca gctgactaag atcgagccgg gcgagctgta taacggcacc aaagtgacca
    41521 agatggaaga tgacatcaaa aagcttctcg gtcgctatgg ttatgcctat ccgcgcgtac
    41581 agtcgatgcc tgaaattaac gatgccgaca aaaccgttaa attacgcgtg aacgttgatg
    41641 cgggtaaccg tttctacgtg cgtaagatcc gttttgaagg taacgatacc tcgaaagatg
    41701 ccgtcctgcg tcgcgaaatg cgtcagatgg aaggtgcatg gctgggaagc gatctggtcg
    41761 atcagggtaa ggagcgtctg aatcgtctgg gcttctttga aactgtcgat accgataccc
    41821 aacgtgttcc gggtagcccg gaccaagtag atgtagtcta caaggtgaaa gaacgtaaca
    41881 ccggtagctt caacttcggg attggttacg gtactgaaag tggcgtgagc ttccaggctg
    41941 gcgtgcaaca ggataactgg ttaggtacag gttatgctgt tggtatcaac gggaccaaaa
    42001 acgattacca gacctatgct gaattgtcgg taaccaaccc gtacttcact gtagatggtg
    42061 taagcctcgg tggtcgtctc ttctataatg acttccaggc agatgacgcc gacctgtccg
    42121 actataccaa caagagttat ggtacagacg tgacgttggg cttcccgatc aacgaatata
    42181 actcgctgcg tgcaggtctg ggttatgtac ataactccct gtccaacatg cagcctcagg
    42241 ttgcgatgtg gcgttatctg tactctatgg gtgaacatcc gagcacctct gatcaggata
    42301 acagcttcaa aacggacgac ttcacgttca actatggttg gacctataac aagcttgacc
    42361 gtggttactt cccgacagat ggttcacgtg tcaacctgac cggtaaagtg accattcctg
    42421 gatcggataa cgaatactac aaagtgacgt tagacacggc gacttatgtg ccgatcgatg
    42481 acgatcacaa atgggttgtt ctggggcgta cccgctgggg ttatggtgat ggtttaggcg
    42541 gcaaagagat gccgttctac gagaacttct atgccggtgg ttccagcacc gtccgtggct
    42601 tccagtccaa taccattggt ccgaaagcag tttacttccc gcatcaggcc agtaattatg
    42661 atccggacta tgattacgaa tgtgcgactc aggacggcgc gaaagacctg tgtaaatcgg
    42721 atgatgctgt aggcggtaac gccatggcgg ttgccagcct cgagttcatc accccgacgc
    42781 cgtttattag cgataagtat gctaactcgg ttcgtacttc cttcttctgg gatatgggta
    42841 ccgtttggga tacaaactgg gattccagcc aatattctgg atatccggac tatagtgatc
    42901 caagcaatat ccgtatgtct gcgggtatcg cattacaatg gatgtcccca ttggggccgt
    42961 tggtgttctc ctacgcccag ccgttcaaaa agtacgatgg agacaaggca gaacagttcc
    43021 agtttaacat cggtaaaacc tggtaagtgt tctccacaaa ggaatgtagt ggtagtgtag
    43081 cgatgacttt aggcgatcaa tataagatcg ccgggccacg caaagaactg caccctccgg
    43141 tgcaaatggg atggtaagga gtttattgtg aaaaagtggt tattagcggc aggtctcggt
    43201 ttagcactgg caacttctgc tcaggcggct gacaaaattg caatcgtcaa tatgggcagc
    43261 ctgttccagc aggtagcgca gaaaaccggt gtttctaaca cgctggaaaa tgagttcaaa
    43321 ggccgtgcca gcgaactgca gcgtatggaa accgatctgc aggctaaaat gaaaaagctg
    43381 cagtccatga aagcgggcag cgatcgcact aagctggaaa aagacgtgat ggctcagcgc
    43441 cagacttttg ctcagaaagc gcaggctttt gagcaggatc gcgcacgtcg ttccaacgaa
    43501 gaacgcggca aactggttac tcgtatccag actgctgtga aatccgttgc caacagccag
    43561 gatatcgatc tggttgttga tgcaaacgcc gttgcttaca acagcagcga tgtaaaagac
    43621 atcactgccg acgtactgaa acaggttaaa taagtaatgc cttcaattcg actggctgat
    43681 ttagcgcagc agttggatgc agaactacac ggtgatggcg atatcgtcat caccggcgtt
    43741 gcgtccatgc aatctgcaca aacaggtcac attacgttca tggttaaccc aaaataccgt
    43801 gagcatttag gcttgtgcca ggcgtccgcg gttgtcatga cccaggacga tcttcctttc
    43861 gcgaaaagtg ccgcgctggt agtgaagaat ccctacctga cttacgcgcg catggcgcaa
    43921 attttagata ccacgccgca gcccgcgcag aacattgcac ccagtgcggt gatcgacgcg
    43981 acggcgaagc tggggaacaa tgtatcgatt ggcgctaacg cggtgattga atccggcgtt
    44041 gaactgggcg ataacgtgat tatcggtgcc ggttgcttcg taggtaaaaa cagcaaaatc
    44101 ggtgcaggtt cgcgtctctg ggcgaacgta accatttacc atgagatcca gatcggtcag
    44161 aattgcctga tccagtccgg aacagtggta ggcgcagacg gctttggtta tgccaacgat
    44221 cgtggtaact gggttaagat cccacagatt ggtcgcgtaa ttattggcga tcgcgtggag
    44281 atcggtgcct gcactaccat cgatcgcggc gcactggatg acactgttat tggcaatggc
    44341 gtgatcattg ataaccagtg ccagattgca cataacgtcg tgattggcga caatacggcg
    44401 gttgccggtg gcgtcattat ggcgggcagc ctgaaaattg gtcgttactg catgatcggc
    44461 ggagccagcg taatcaacgg gcatatggaa atatgcgaca aagtgacggt tacgggcatg
    44521 ggtatggtga tgcgtcccat cactgaacca ggcgtctatt cctcaggcat tccgctgcaa
    44581 cccaacaaag tctggcgcaa aaccgctgca ctggtgatga acattgatga catgagcaag
    44641 cgtctgaaat cgcttgagcg caaggttaat caacaagact aacgttccat cttttgttcg
    44701 ccaaacttta cggcctgtct cattcttacg attgcggcag gccgtgttat tattgtcgtt
    44761 tcttatattt tgacaggaag agtatcttga ctactaacac tcatactctg cagattgaag
    44821 agattttaga acttctgccg caccgtttcc cgttcttact ggtggatcgc gtgctggatt
    44881 ttgaagaagg tcgttttctg cgcgcagtaa aaaatgtctc tgtcaatgag ccattcttcc
    44941 agggccattt ccctggaaaa ccgattttcc cgggtgtgct gattctggaa gcaatggcac
    45001 aggcaacagg tattctggcg tttaaaagcg taggaaaact ggaaccgggt gagctgtact
    45061 acttcgctgg tattgacgaa gcgcgcttca agcgcccggt cgtgcctggc gatcaaatga
    45121 tcatggaagt cactttcgaa aaaacgcgcc gcggcctgac ccgttttaaa ggggttgctc
    45181 tggtcgatgg taaagtagtt tgcgaagcaa cgatgatgtg tgctcgtagc cgggaggcct
    45241 gatacgtgat tgataaatcc gcctttgtgc atccaaccgc cattgtggaa gagggcgcgt
    45301 caattggcgc gaacgcacac attggtcctt tttgtatcgt tggaccccat gtcggaattg
    45361 gtgagggtac cgtactgaaa tctcacgttg tcgtgaatgg tcatactaaa attggccgcg
    45421 ataatgagat ttatcagttc gcctccatcg gcgaagttaa ccaggatctg aaatatgctg
    45481 gcgaaccgac ccgtgtggaa atcggcgatc gtaaccgcat tcgcgaaagc gtcaccattc
    45541 atcgtggcac agtccagggc ggtggattga cgaaggtggg cagcgacaac ttactgatga
    45601 tcaacgcgca cattgcgcac gattgtacgg taggtaaccg ctgtattctc gccaacaacg
    45661 caacgctggc gggtcacgta tcggttgacg acttcgcgat catcggcggc atgaccgcag
    45721 tccatcagtt ctgcatcatt ggtgcgcacg tgatggttgg cggctgctcc ggtgtggcgc
    45781 aggacgtccc tccttatgtc attgcgcagg gtaaccacgc aacgccgttc ggtgtcaata
    45841 tcgaagggct gaagcgccgc ggattcagcc gtgaggcgat taccgctatc cgcaatgcgt
    45901 ataagctgat ttatcgtagc ggtaaaacgc tcgatgaagt gaaaccggaa attgctgaac
    45961 tggcggaaac atatccggaa gtgaaagcct ttaccgattt cttttcacgc tcaacgcgcg
    46021 gtctgattcg ttaatgactg aacagcgtcc attaacgatt gccctggtcg ccggagaaac
    46081 ctccggcgat atcctagggg ccggtttaat ccgcgctctg aaagaacgtg tgcccaacgc
    46141 ccgctttgtt ggtgttgccg ggccacgaat gcaggctgaa ggctgcgaag cctggtacga
    46201 aatggaagaa ctggcggtga tgggcattgt tgaagtgctc ggtcgtctgc gtcgcttact
    46261 gcatattcgt gccgatctga caaagcgttt tggcgaactg aagccagatg tttttgttgg
    46321 tattgatgcg cctgacttca atattactct tgaaggtaac ctcaaaaagc agggtatcaa
    46381 aaccattcat tacgtcagtc cgtcagtctg ggcgtggcga cagaaacgtg ttttcaaaat
    46441 aggcagagcc accgatctgg tgctcgcatt tctgcctttc gaaaaagcgt tttatgacaa
    46501 atacaacgta ccgtgccgct ttatcggtca taccatggct gatgccatgc cattagatcc
    46561 agataaaaat gccgcccgtg atgtgctggg gatccctcac gatgcccact gcctggcgtt
    46621 gctaccgggg agccgtggtg cagaagttga aatgcttagt gccgatttcc tgaaaacggc
    46681 ccagcttttg cgccagacat atccggatct cgaaatcgtg gtgccactgg tgaatgccaa
    46741 acgccgcgag cagtttgaac gcatcaaagc tgaagtcgcg ccagactttt cagttcattt
    46801 gctggatggg atgggccgtg aggcgatggt cgccagcgat gcggcgctac tggcgtcggg
    46861 tacggcagcc ctggagtgta tgctggtgaa atgcccgatg gtggtgggat atcgcatgaa
    46921 gccttttacc ttctggttgg cgaagcggct ggtgaaaact gattatgtct cgctgccaaa
    46981 tctgctggcg ggcagagagt tagtcaaaga gttattgcag gaagagtgtg agccgcaaaa
    47041 actggctgcg gcgctgttac cgctgttggc gaacgggaaa atcagccacg cgatgcacga
    47101 taccttccgt gaactgcatc agcagatccg ttgcaatgcc gatgagcagg cggcacaagc
    47161 cgttctggag ttagcacaat gatcgaattt gtttatccgc acacgcagct ggttgcgggt
    47221 gtggatgaag tcggacgcgg gccgttagtt ggcgcggtcg tcaccgctgc ggtgatcctt
    47281 gacccggcgc gcccgattgc cgggctgaat gattccaaaa agctgagcga aaaacgccgt
    47341 ctggcgctct gtgaagagat caaagagaaa gcgttgagct ggagtctggg ccgcgcggaa
    47401 ccccacgaaa tcgacgagct gaacattctt catgcgacca tgctggcgat gcagcgtgcc
    47461 gtcgctgggc tgcatattgc gccggaatat gtgttgattg atggtaaccg ctgcccgaaa
    47521 ttaccgatgc ctgcgatggt tgtggtgaaa ggcgatagcc gcgtaccgga aatcagtgcc
    47581 gcgtctatcc tggcgaaagt gacgcgtgac gccgaaatgg cggcgctgga tattgttttc
    47641 ccgcaatatg gttttgccca acacaaaggg tacccaaccg cttttcatct ggaaaaactg
    47701 gctgaacacg gcgcgaccga acaccatcgg cgcagctttg ggcctgtcaa acgcgcactg
    47761 ggacttgcgt cctgattctt gtgtcgagat taagtaaacc ggaatctgaa gatgtctgaa
    47821 ccacgtttcg tacacctgcg ggtgcacagc gactactcga tgatcgatgg cctggccaaa
    47881 actgcaccgt tggtaaaaaa ggcggcggcg ttgggtatgc cagcactggc gatcaccgat
    47941 ttcaccaacc tttgtggtct ggtgaagttc tacggagcgg gacatggcgc agggattaag
    48001 cctatcgtcg gggcagattt taacgtccag tgcgacctgc tgggtgatga gttaacccac
    48061 ctgacggtac tggcggcgaa caataccggc tatcagaatc tgacgttgct gatctcaaaa
    48121 gcgtatcagc gcgggtacgg tgccgctggg ccgatcatcg atcgcgactg gcttatcgaa
    48181 ttaaatgaag ggttgatcct tctttccggc gggcgcatgg gcgacgtcgg acgcagtctt
    48241 ttgcgtggta acagcgcgct ggtagatgag tgtgtcgcgt tttatgaaga acacttcccg
    48301 gatcgctatt ttctcgagct gatccgcacc ggcaggccgg atgaagaaag ctatctgcac
    48361 gcggcggtgg aactggcgga agcgcgcggt ttgcccgtcg tggcgactaa cgatgtgcgc
    48421 tttatcgaca gcagcgactt tgacgcacac gaaatccgcg tcgcgatcca cgacggcttt
    48481 accctcgacg atcctaaacg cccgcgtaac tattcgccgc agcaatatat gcgtagcgaa
    48541 gaggagatgt gtgagctgtt tgccgacatc cccgaagccc ttgccaacac cgttgagatc
    48601 gccaaacgct gtaacgtaac tgtgcgtctt ggtgaatact tcctgccgca gttcccgacc
    48661 ggggacatga gcaccgaaga ttatctggtc aagcgtgcaa aagagggcct ggaagagcgt
    48721 ctggcctttt tattccctga cgaggaagaa cgtgttaagc gccgcccgga atatgacgaa
    48781 cgtctggaga ctgaacttca ggttatcaac cagatgggct tcccgggcta cttcctcatc
    48841 gttatggaat ttatccagtg gtcgaaagat aacggcgtac cggtagggcc aggccgtggc
    48901 tccggtgcgg gttcactggt ggcctacgcg ctgaaaatca ccgacctcga tccgctggaa
    48961 tttgacctgc tgttcgaacg tttccttaac ccggaacgtg tctccatgcc tgacttcgac
    49021 gttgacttct gtatggagaa acgcgatcag gttatcgagc acgtagcgga catgtacggt
    49081 cgtgatgcgg tatcgcagat catcaccttc ggtacaatgg cggcgaaagc ggtgatccgc
    49141 gacgtaggcc gcgtgctggg gcatccgtac ggctttgtcg atcgtatctc gaaactgatc
    49201 ccgcccgatc cggggatgac gctggcgaaa gcgtttgaag ccgagccgca gctgccggaa
    49261 atctacgaag cggatgaaga agttaaggcg ctgatcgaca tggcgcgcaa actggaaggg
    49321 gtcacccgta acgccggtaa gcacgccggt ggggtggtta tcgcgccgac caaaattacc
    49381 gattttgcgc cgctttactg cgatgaagag ggcaaacatc cggtcaccca gtttgataaa
    49441 agcgacgttg aatacgccgg actggtgaag ttcgacttcc ttggtttgcg tacgctcacc
    49501 atcatcaact gggcgctgga gatgatcaac aagcggcggg cgaagaatgg cgagccgccg
    49561 ctggatatcg ccgcgatccc gctggatgac aagaaaagct tcgacatgct gcaacgctcg
    49621 gaaaccacgg cggtattcca gcttgaatcg cgcggcatga aggatctgat caagcgtctg
    49681 caacctgact gcttcgaaga tatgatcgca ctggtggcgc tgttccgccc aggtccgttg
    49741 caatcaggga tggtggataa ctttatcgac cgtaaacatg gtcgcgaaga aatctcctat
    49801 ccggacgtac agtggcagca tgaaagcctg aaaccggtac tggagccaac ctacggcatc
    49861 atcctgtatc aggaacaggt catgcagatt gcgcaggtgc tttctggtta taccctcggt
    49921 ggcgcggata tgctgcgtcg tgcgatgggt aagaaaaagc cggaagagat ggctaagcag
    49981 cgttctgtat ttgctgaagg tgcagaaaag aacggagtca acgccgaact ggcgatgaaa
    50041 atcttcgacc tggtggagaa attcgcgggt tacggattta acaaatcgca ctctgcggcc
    50101 tatgctttgg tgtcatatca aacgttatgg ctgaaagcgc actatccggc ggagtttatg
    50161 gcggcggtaa tgaccgccga tatggacaac accgagaagg tggtgggcct ggtggatgag
    50221 tgctggcgga tggggctgaa aatcctgcca ccagatataa actccggtct ttaccatttc
    50281 cacgtcaacg acgacggcga aatcgtgtat ggtattggcg ccatcaaagg ggtaggtgaa
    50341 ggtccgattg aggccatcat cgaagcccgt aataaaggcg gctacttccg cgaactgttt
    50401 gatctctgcg cccgtaccga caccaaaaag ttaaaccggc gggtgctgga aaaactgatc
    50461 atgtccgggg cgtttgaccg tcttgggcca caccgcgcgg cgctgatgaa ctcgctgggt
    50521 gatgcgttaa aagcggcaga tcaacacgcg aaagcggaag ctatcggtca ggccgatatg
    50581 ttcggcgtgc tggccgaaga gccggaacaa attgaacaat cctacgccag ctgccaaccg
    50641 tggccggagc aggtggtgtt agatggggaa cgtgaaacgt taggtctgta ccttacggga
    50701 caccctatca accagtattt aaaagagatt gagcgttatg tcggaggcgt aaggctgaaa
    50761 gacatgcacc cgacagaacg tggtaaagtc atcacggctg cggggctcgt tgttgccgcg
    50821 cgggttatgg tcaccaagcg cggcaatcgt atcggtatct gcacgctgga tgaccgttcc
    50881 gggcggctgg aagtgatgtt gtttactgac gccctggata aataccagca attgctggaa
    50941 aaagaccgca tacttatcgt cagcggacag gtcagctttg atgacttcag cggtgggctt
    51001 aaaatgaccg ctcgcgaagt gatggatatt gacgaagccc gggaaaaata tgctagcggg
    51061 cttgctatct cgctgacgga caggcaaatt gatgaccagc ttttaaaccg actccgtcag
    51121 tctctggaac cccaccgctc tgggacaatt ccagtacatc tctactatca gagggcggat
    51181 gcacgcgcgc ggttgcgttt tggcgcgacg tggcgtgtct ctccgagcga tcgtttatta
    51241 aacgatctcc gtggcctcat tggttcggag caggtggaac tggagtttga ctaatacagg
    51301 aatactatga gtctgaattt ccttgatttt gaacagccga ttgcagagct ggaagcgaaa
    51361 atcgattctc tgactgcggt tagccgtcag gatgagaaac tggatattaa catcgatgaa
    51421 gaagtgcatc gtctgcgtga aaaaagcgta gaactgacac gtaaaatctt cgccgatctc
    51481 ggtgcatggc agattgcgca actggcacgc catccacagc gtccttatac cctggattac
    51541 gttcgcctgg catttgatga atttgacgaa ctggctggcg accgcgcgta tgcagacgat
    51601 aaagctatcg tcggtggtat cgcccgtctt gacggtcgtc cggtgatgat cattggtcat
    51661 caaaaaggtc gtgaaaccaa agaaaaaatt cgccgtaact ttggtatgcc agcgccagaa
    51721 ggttaccgca aagcactgcg tctgatgcaa atggctgaac gctttaagat gcctatcatc
    51781 acctttatcg acaccccggg ggcttatcct ggcgtgggcg cagaagagcg tggtcagtct
    51841 gaagccattg cacgcaacct gcgtgaaatg tctcgcctcg gcgtaccggt agtttgtacg
    51901 gttatcggtg aaggtggttc tggcggtgcg ctggcgattg gcgtgggcga taaagtgaat
    51961 atgctgcaat acagctccta ttccgttatc tcgccggaag gttgtgcgtc cattctgtgg
    52021 aagagcgccg acaaagcgcc gctggcggct gaagcgatgg gtatcatcgc tccgcgtctg
    52081 aaagagctga aactgatcga ctccatcatc ccggaaccgc tgggcggcgc tcaccgtaac
    52141 ccggaagcga tggcggcatc gttgaaagcg caactgctgg cggatctggc cgatctcgat
    52201 gtgttaagca ctgaagattt aaaaaatcgt cgttatcagc gcctgatgag ctacggttac
    52261 gcgtaattcg caaaagttct gaaaaagggt cacttcggtg gccctttttt atcgccacgg
    52321 tttgagcagg ctatgattaa ggaaggattt tccaggagga acacatgaac atcattgcca
    52381 ttatgggacc gcatggcgtc ttttataaag atgagcccat caaagaactg gagtcggcgc
    52441 tggtggcgca aggctttcag attatctggc cacaaaacag tgttgatttg ctgaaattta
    52501 tcgaacataa ccctcgaatt tgcggcgtga tttttgactg ggatgagtac agtctcgatt
    52561 tatgcagcga tatcaatcaa cttaatgaat atctcccgct ttatgccttc atcaacaccc
    52621 actcgacgat ggatgtcagc gtgcaggata tgcggatggc gctctggttt tttgaatatg
    52681 cgctggggca ggcggaagat atcgccattc gtatgcgtca gtacaccaac gaatatcttg
    52741 ataacattac gccgccgttc acgaaagcct tgtttaccta cgttaaagag cggaagtaca
    52801 ccttttgtac gccggggcat atgggcggca ccgcatatca aaaaagcccg gttggctgcc
    52861 tgttttatga ttttttcggc gggaataccc tcaaggctga tgtctctatt tcggtcaccg
    52921 agcttggttc gttgctcgac cacaccgggc cacacctgga agcggaagag tacatcgcgc
    52981 ggacttttgg cgcggaacag agttatatcg ttaccaacgg aacatcgacg tcgaacaaaa
    53041 ttgtgggtat gtacgccgcg ccatccggca gtacgctgtt gatcgaccgc aattgtcata
    53101 aatcgctggc gcatctgttg atgatgaacg atgtagtgcc agtctggctg aaaccgacgc
    53161 gtaatgcgtt ggggattctt ggtgggatcc cgcaccgtga atttactcgc gacagcatcg
    53221 aagagaaagt cgctgccacc acgcaggcac aatggccggt tcatgcggtg attaccaact
    53281 ccacctatga tggcttgctc tacaacaccg actggatcaa acagacgctg gatgtcccgt
    53341 cgatccactt cgattccgcc tgggtgccgt acacccattt tcatccgatc taccagggga
    53401 aaagtgatct tcgaaacgca atcgacccac aaaatgctgg cggcgttatc gcaggcttcg
    53461 ctgatccaca ttaaaggcga gtatgacgaa gaggcgttta acgaagcctt tatgatgcat
    53521 accaccacct cgcccagtta tcccattgtt gcttcggttg agacggcggc ggcgatgctg
    53581 cgtggtaatc cgggcaaacg gctgattaac cgttcggtgg agcgggcgct gcattttcgc
    53641 aaagaggtcc agcggttgcg ggaagagtct gacggttggt ttttcgatat ctggcaaccg
    53701 ccgcaggtgg atgaagccga atgctggccc gttgcgccag gcgaacagtg gcacggcttt
    53761 aacgatgcgg atgccgatca tatgtttctc gatccggtta aagtcactat tttgataccg
    53821 gggatggacg aacagggcaa tatgagcgag gaggggatcc cggcggcgct ggtggcaaaa
    53881 ttcctcgacg aacgtgggat cgtagtagag aaaaccggcc cttataacct gctgtttctc
    53941 tttagtattg gtatcgataa aaccaaagca atgggattat tgcgtgggtt gacggaattt
    54001 aaacgctctt acgatctcaa cctgcggatc aaaaatatgc tacccgatct ctatgctgaa
    54061 gatcccgatt tctaccgcaa tatgcgtatt caggatctgg cgcaagggat ccataagctg
    54121 attcgtaaac acgatcttcc cggtttgatg ttgtgggcat tcgatacttt gccggaaatt
    54181 atcatgacgc cacatcaggc atggcagaga cagattaaag gtgaagtaga aaccattgcg
    54241 ctggaacaac tggtcggtag agtatcggca aatatgagcc tgccttatcc accgggcgta
    54301 ccgctgttga tgccaggaga aatgctgacc aaagagagcc gcacggtact cgattttcta
    54361 ctgatgcttt gttccgtagg gcaacattac cccggttttg aaacggatat tcacggcgcg
    54421 aaacaggacg aagacggcgt ttaccgcgta cgagtcctaa aaatggcggg ataacttgcc
    54481 agagcggctt acgggcgagt aacgtgctgt taacaaataa aggagacgtt atgctgggtt
    54541 taaaacaggt tcaccatatt gcgattattg cgacggatta tgcggtgagc aaagctttct
    54601 actgcgatat tcttggtttc acgctgcaaa gcgaagtcta tcgcgaagcg cgcgactcgt
    54661 ggaaagggga tttggcgctt aatgggcaat atgtgattga gcttttctct tttccgttcc
    54721 cgccggagcg ccccagccga ccggaagctt gcggcctgcg tcatctggct tttagcgttg
    54781 atgacatcga tgcggcagtg gcgcaccttg aaagccataa cgtgaagtgt gaagccatcc
    54841 gtgtcgatcc atacacgcaa aaacgcttca ccttctttaa cgatccggac gggctgccgt
    54901 tggaactgta tgagcagtaa ggcttgtcat cgccgcattt gcccggtaac gtgccgggca
    54961 ttgctactgt aaaatcgcac catcatgaca ctcacgctca atagacaact tctcacctca
    55021 cgccagattt tggtcgcctt tagcggcggg cttgactcca ccgttctgct gcatcagttg
    55081 gtgcagtggc ggacggaaaa tccgggcgtc actctgcgcg ctattcatgt gcatcacggt
    55141 ttaagtgcca atgccgatgc ctgggttacg cattgcgaaa acgtctgcca acagtggcag
    55201 gtgccgctgg tggtcgaacg cgtacaactt gcgcaagagg ggctggggat tgaggcccag
    55261 gcgcggcagg cgcgttatca ggcatttgcc cgtaccctat tgcccggcga agtgctggtc
    55321 accgcgcaac atctcgacga tcaatgtgaa acctttctgc tggcgctaaa acgcggtagc
    55381 ggccctgccg ggctttcggc tatggcggaa gtttcggagt ttgccggaac acggcttatc
    55441 cgcccgttgc ttgcccgcac gcggggggaa ctggcgcagt gggcattggc gcatggttta
    55501 cgctggattg aagacgaaag taatcaggac gactcatacg atcgtaactt tctgcgcctg
    55561 cgcgtagtgc cgttattgca gcagcgttgg ccgcattttg cggaagcaac ggcccgcagc
    55621 gccgcacttt gtgctgaaca agagagtctg ttggatgaac tgctggcgga tgatttagca
    55681 cactgccaaa cgtcgcaggg gacgctgcag attgcgccaa tgctggcgat gagtgatgcc
    55741 cgccgcgcgg cgattatccg tcgctggctg gcagggcaga atgcaccaat gccttcccgc
    55801 gaagcgctgg tgcggatctg gcaggaagtg gcgctggcgc gggaagatgc ctccccctgt
    55861 ttacgcttgg gcgcgtttga aatccgccgc tatcagtcgc aactgtggtg gattaaatcc
    55921 gtcaccgggc aaagtgaaac cattgtgccg tggcagacgt ggctacaacc gctggaatta
    55981 ccggcggggc tgggaagcgt acagcttact gcgggaggcg atattcgccc tccgcgtgca
    56041 gacgaagcgg tcagcgtgcg tttcaaatcg gcaggattgc tgcatattgt cgggcgcaac
    56101 ggcggacgta agctgaagaa aatctggcaa gagttaggcg tgccgccgtg gctacgtgac
    56161 accacgccac tgctgtttta tggcgaaacg ctgattgcgg cggcaggggt atttgtgacg
    56221 caagaaggtg tggctgaagg tgagaatggc gtaagttttg tctggcagaa aacgcttagt
    56281 taagtgaaag ccggataaga cgcatcaaac gtcgcatccg gcgaaagtca atcaggactc
    56341 gctcaccacc accgtaccga tttccgggtg gctaaagctg gtaattttat ccagacgtag
    56401 ctcacgggtt tcgccagcgg cctcgacgac cagatactcc acatttttgc gggagactaa
    56461 atcgctggct tttgcctgca atttttcgcc atctttcagc tcaagtgtca gcattaaatg
    56521 atgctggcag gcgagctcaa gattatcgta atcatcacaa ttgattggtt gatacgtatc
    56581 attcattaac ataatcgctc accagtaagt ttgccgcagc gtatgctgct ttttccctga
    56641 cagcctcaga aagggcgtcg tcggcagcca tttcattcag cactttcaaa acgcagccca
    56701 gcgcgtccgg aacgtatcct aagtctccgc tggcgatttc cgcgtaccgc ttgcgtatta
    56761 actcacaata tttttccaca tgccctcctg tcagcacact gacttaaccg tggatgcaag
    56821 tctaagccta cgaagataaa ctctgtttcg caaggtgact ataccacact catttctgca
    56881 atatcagcgc cgcaactgca cgtattccgt tacaatggcc tcctgattcg aaaggagttt
    56941 tcttatggcg cttaaagcga caatttataa agcgacggtt aatgtggccg atctcgaccg
    57001 caaccagttt ctcgatgcct ctctgacgct ggcgcgccac ccttcagaaa cccaggagcg
    57061 tatgatgctg cgcctgctgg cgtggctgaa atatgctgat gaacgtctgc aatttacccg
    57121 tggtttgtgt gccgatgatg agccggaagc gtggctgcgt aacgatcacc tgggcattga
    57181 tttgtggatt gagctggggc tgccggatga gcggcggatt aagaaagcct gcacccaggc
    57241 cgcagaagtg gcgctgttta cctataatag tcgggcggcg caaatctggt ggcagcaaaa
    57301 tcagagcaaa tgtgtgcagt ttgccaatct ttccgtctgg tatctggacg atgaacaact
    57361 ggcgaaagta agcgcctttg ccgatcgtac catgacgctg caggcaacga ttcaggatgg
    57421 cgtgatctgg ttatcggacg ataagaataa tctggaagtt aacttaaccg cctggcaaca
    57481 accttcatga ttgtgatttc ccgacatgtt gctattcccg atggtgagct tgagatcacc
    57541 gccattcgtg cgcagggcgc gggcgggcag catgttaata agacctcaac ggccattcat
    57601 ctgcgttttg acattcgggc gtccagcctg ccagagtatt acaaagagcg tctgctcgcc
    57661 gccagccatc atttgatcag cagtgatggc gtgattgtca ttaaggcaca ggaataccgc
    57721 agtcaggaac tgaaccgcga agccgcgcta gcccggctgg tggcagtgat taaagattta
    57781 acaacagaac aaaaagcccg acgacccacg cggcccaccc gtgcatcgaa agagcgcagg
    57841 ctggcatcga aagcacaaaa atcaagcgcg aaggcgatgc gcggcaaagt acgcagcggt
    57901 cgggaataaa aagaaggaat agatggtgaa aaaagcgata gtgacagcga tggctgtaat
    57961 cagcctcttt actctgatgg gatgtaataa ccgggccgaa gtcgatacgc tttctccagc
    58021 acaggctgcc gaactgaaac cgatgccgca aagttggcgc ggcgtgctgc cgtgtgctga
    58081 ttgtgaagga atcgaaacct ctctgttcct cgaaaaagac ggaacatggg tgatgaatga
    58141 gcgttatctc ggtgctcgtg aagaaccttc ctccttcgct tcctacggta catgggcgcg
    58201 aaccgctgac aagctggtat taaccgacag caaaggtgaa aagtcatatt atcgggcgaa
    58261 aggcgatgcg ctggagatgc tcgatcgtga aggcaatccg attgaatcgc agttcaacta
    58321 tacgctggaa ccggcacaat ccagtttacc tatgacgccg atgaccctgc gaggcatgta
    58381 tttttatatg gctgatgcgg cgaccttcac tgattgcgcg accggaaaac gtttcatggt
    58441 agcgaataac gcagagctgg agcgtggcta cctggctgcg cgcggtcaca gtgaaaaacc
    58501 ggtgttactg tcagtagaag gtcactttac gcttgaggct aatccggata ctggtgcgcc
    58561 aaccaaagta ttagcgcccg atacggcggg taaattttac ccgaataagg attgcagtag
    58621 tttggggcag taaacctgct taaggcagaa acaaacgcaa aacaggccag aaggatatat
    58681 ttcaacattt tgaatttgca cgttttttgt aggccggata aggcgtttac gccgcatccg
    58741 gcaatggtgc tcaaagcctg atgcgacgct ggcgcgtctt atcatgccta caacccccct
    58801 cataccttaa ggcggataag gcatttactt tatcaccggg tcaaaccaac aaagcgaccc
    58861 gccttgatat aaatccctgg cttcaggtgc ccaacatact gtaactgcgt ttctggctta
    58921 aacgcagaga catcaccaat acgcatatgc atcagatcgg cagggctaat ccagcccgat
    58981 tgcgccagtg taagtggatg tccggctttg gcaaatgcat cagtgacaaa ttccgaacaa
    59041 aaccacgact ttttgtctcc ttcgcccaca ctgcttaact gcgctttcgc caggccgctg
    59101 acgcactgct ggcgaaaatc ctcggagaac tgattcagtg agcacatctg gcgagtcacc
    59161 ataaagggaa taaattcgac aatgccgcga tagttataac cgctatcttt gattttattg
    59221 gcaaacgcgg tgatatctgt ggcttgttgc ggggtaagat ccgggactcg taagacgaaa
    59281 agcttatcac tatgcttcat cgctttttta agggaaacaa tctggacgcc agcgcctgtc
    59341 gcttctgcaa cgttattatc acccagaaag attgcaacgt gactcacaga ggaagtgctg
    59401 aagacgcgga ttccaaatga ggttacccca aggcttgagg agaacagcaa atcgccgggt
    59461 ttgagatcag gtgccgttat ttcttttatt gattgttcgg tgaaagagct ttgatgctgg
    59521 aatttgacag cccatgtttt tgcctcggca tctactgctg tggctgatgg ggctggctga
    59581 tatcaacggt acaagccgaa agtaaaagaa agctggggag aagcaggcgg cagtacgcct
    59641 ttggtttatc cattttatac aatccatgta aaaaagggcc ctgaaattca ggaccctttc
    59701 tggcatcagc ctttaatctg tttcaccaga tattcgacga tgtcaccagt cttaattaac
    59761 tgtttctcgc cgttgcgacg atatttatat tcgatatcgt cgttgtcgag gttacggtcg
    59821 cccagcacaa tagtgtgcgg aataccgatc agttccatat cagcaaacat cacgcccgga
    59881 cgctctttgc ggtcatccag cagcacttcg ataccttgtg cacgcagttc gctgtacagt
    59941 ttctcagcaa gctcttgtac gcggaaggat ttgtgcatgt tcatcggcag aatcgccacc
    60001 tggaacggcg cgatagcgtc aggccatacg atgccgcgtt cgtcgtagtt ctgctcaatc
    60061 gccgcagcta ccacacgcgt taccccgata ccgtagcaac ccatcgtcag gatttggtta
    60121 cggccatctt caccctgtac ggaggctttc agtgcttcgg agtacttggt acccagctgg
    60181 aagatgtgac caacttcgat accacgtttg atcagcagcg taccctggcc atccgggctt
    60241 ggatcgccag ccaccacgtt acggatatct gcaacttccg gggtagcgac atcgcgatcc
    60301 cagttgatac cgaagtagtg tttaccatcg atgttagcac cagcagcgaa atcactcatc
    60361 gccgcaacgg tacggtcaat caccaccgga atcggcatgt ttaccggacc cagtgaaccc
    60421 ggaccggctt taaccacagc acgaatttct tcttcggtcg cgaaagtcag cgggcttgca
    60481 acctgcggca gtttttctgc tttaacttcg ttcagctcgt gatcaccgcg caccagcagc
    60541 gcaaccagcg ggaagctgct gccttcaacc gctttaacca gcagagtctt aaccgttttc
    60601 tcaatcggca gattgaactg ttcaaccagt tccgcgatgg ttttcgcgtt cggcgtatca
    60661 accagcgtca tttcctgggt agcagcagcg cgcggttctt tcggcgcgat agcttctgcc
    60721 agttcaatgt tcgctgcata gtcagaggtg tcggagaaga ccacatcgtc ttcaccgctc
    60781 tgcgccagca cctggaattc gtgagaggcg ctgccgccga tagaaccggt gtcggcttgc
    60841 acggcgcgga aatccagccc catgcggctg aagattttgc tgtaggccgc atacattgca
    60901 tcgtaggttt cctgcaggga ttcctgagaa gtatggaaag agtaagcatc tttcatcagg
    60961 aattcgcggg aacgcatgac gccgaaacgc ggacgcactt cgtcgcggaa cttggtctgg
    61021 atctgataga agttcagcgg cagctgtttg taagagctaa gctcgttacg aatcaggtca
    61081 gtgataactt cttcatgagt tgggccgagt acgaacggac gctcgccacg gtcaacaaaa
    61141 cgcagcagtt ccggaccgta ctgttcccaa cgaccactct cttgccacaa atcggctggc
    61201 tgaaccaccg gcatcgacac ctcgattgca ccggcgttgt tcatctcttc acgcacgatg
    61261 ttttcgactt ttttcagaac gcgcacgccg gtcggcagcc aggtatataa cccggaggcc
    61321 agcttgcgga tcatcccggc gcgcagcatc agctgatggc tgatcacctc ggcgtcggca
    61381 ggtgtctcct tgagagtgga gagcaggtat tggctagtac gcatgttgtt acggttccag
    61441 ttggaaggta gaacaggctc aaggcgagcc tgggacaaaa aagtgattta gtttaccagt
    61501 gcaaaagaaa tgtcaaaaga gaagggcgtg aatttaacgc ggttccagcg caaagacttc
    61561 aaaacctgcg tcggtgacgc gccagcgaac gttaaaatca tgtagccaga cggcataggt
    61621 tttgcccgtt tcctcacctt tatgataggc cgggcgcggg tcctgcgcca gtacttcgcg
    61681 gataaacagc gttaactgcg gataacgctt ctccagcgtc aaaagctgct tttcgacctc
    61741 tgcggtaaaa ctcaccgcca tctctgcagc tggcgcactt tgcgcatagc tggcactggc
    61801 atcgggaagc gattcggcaa aggggagata cggtttgata tccactaccg gcgtaccatc
    61861 gaccagatcc agactgccga gcttcagaat cacgctgtct ttatggcaaa caacctcttt
    61921 cagctctacc agcgacatac caattgggtt agggcggaaa gtagagcgtg ttgcgaaaac
    61981 ccccattctg gcgttaccgc cgaggcgcgg tggacgcaca gtcggacgcc agccgccttc
    62041 catcgtttga tgaaagacga aaaggatcca taaatggctg aacgcttcca ggccgcgaac
    62101 ggcgtcggcc tggttgtagg gagcaatgag atgcagttcg ccgttggcgc tttttaccag
    62161 acctggctgg cgcggaacgg cgaatttttc tttatagggc gagcgaataa cgcctatttg
    62221 ctcgaactgg aaactgctca tttcgccgta atgttaagcg cagagccgat acatacagcc
    62281 tgacgatagc agcctggcgt accgctggtg acttcgcagc tatgcagtaa taccgcattg
    62341 gctttcattt tagaggcgtt gatttgcatc cgcttacgcg cggttggaat gctcggcgga
    62401 gagtcctgat tagaggcctg gcaagagtcg ccactgactt caccgagatc gcggaacggt
    62461 ttgccgacta attcttctgc attggtataa attcggaccg gcgtggcgcg cggcgctttc
    62521 ggttttgcag gctccgcttt cggctggggt gcagtgcttt gaacgggttc gacaggggat
    62581 ctgcttaaca tggaacagcc gcttagcatg agtgctacta aacagatcgg taaagcacgc
    62641 atagtatttc ctcaatgtat gatcaaaacg tcaatattga atcaggagct tgtaaaaatg
    62701 acaagacggg caagtgccca tcctgaatga tattacaaat tgtggaaaca gcctaaaaat
    62761 taccagcctt taacagcgcc gccgttaaac actttgtttg ctgcttcgta aacttcatca
    62821 gactgataag cctggacgaa tttcttcacg ttctcggcgt ctttgttatc ttcacgcgtt
    62881 acgatcaggt ttacgtacgg ggagtcttta tcttcgacaa agataccgtc tttcgctgga
    62941 gtcaggccaa tctggctggc ataggtggta ttgataactg ccagagcgat ttgtgcgtcg
    63001 tccagagatc gaggcagctg cggtgcttcc agctcaacaa ttttcagatt ttttgggttc
    63061 tcaacaacat caagtacggt cggcagcagg ccaacgccat ctttcagttt gatcaagccc
    63121 actttttgca gcagcagcag tgaacgacca aggttagttg gatcgtttgg cacggcaacc
    63181 tgcgaaccat cctgcagttc atccagtgat ttgattttct tggagtaacc agcaatcgga
    63241 taaacaaaag tgttgcctac tgcgaccagt ttgtagccac gatctttcag ttgctgatca
    63301 aggtacggtt tatgctggaa ggcgttggcg tcgatatcgc ctttgctcaa tgcttcgttt
    63361 ggcagaacat agtcgttgaa ggttaccagc tcaacgtcca ggccatattt gtctttcgca
    63421 actttctgcg cgacttctgc aacctgctgt tcggcaccaa caatcacgcc gactttaatg
    63481 tggtttggat ctttttcatc ctgaccgcag cctaccagtg ccagtgatcc aatcagggct
    63541 cccactgccg caaaggtttt gaatttgaac gccatacctt attccttctt caattattta
    63601 tgttgtgttg aacgttactt gcgagtgaca gcccggacga tgcggtcgcc tgcgaactga
    63661 attaaataaa ccagaatgac cagcaatacc agtaccgtat tcatcaccgt cgcgttatag
    63721 ccgatgtagc catactgata gccaatctga cctaaaccac cggcaccgac agcaccaccc
    63781 atcgcggaat aaccgactag ggtaatcagg gtgatagttg ccgcattcac cagacccggc
    63841 aatgcttccg gtaacagcac tttacggacg atctgcatcg gcgtggcacc cattgcacgg
    63901 gaagcttcaa ttaacccggt tgggatctcc agcagagcgt tctcgaccat acgggcaata
    63961 aacggtgctg caccaacggt taacggaaca atcgctgcct gcaaaccaat cgatgtacca
    64021 acaataacgc gggtaaacgg aatcatccat accagcaaga taatgaacgg gatagaacgg
    64081 aaaatgttca caatcgcaga aacggtacga tacagcttcg cgttagcaat aatttgcccc
    64141 ggacgcgtga cataaagcag aacgccaacc ggcagaccaa tcacaaagcc aaaaaaaccg
    64201 gatacgaagg tcattgccag cgtttcccat acgccacgaa ccagcagcca catcatcggc
    64261 tcagacataa cccagtacct ctacttttac atggtgttcc tgcagccagg caatggcggc
    64321 ttgcgtatct tgttgtgtgc cgtgcatttc agtcagcatg atgccgaact taacgccacc
    64381 ggcgtagtcc atctgcgcgc taataatgtt gttgttgacg ttgaaacgac gcgcggtttc
    64441 agaaagcagt ggggcatcga ccgattgacc ggtaaactcc agacgcagca tcggcacgca
    64501 gtcagtaaat ggctccgctt gcagacgttc ctggtaatct tccgggatat ccagatgcag
    64561 ggtcgactga ataaacttct gcgccagcgg cgttttcgga tgcgagaaca cttcacttac
    64621 cgtgtcctgc tcgatcagtt cgccattgct gatgaccgcc acgcaatcac aaatacgctt
    64681 cacaacgtcc atttcgtggg tgatcaacag aatcgtcaaa cccagacggc ggttaatgtc
    64741 tttcagcagt tcgagaatag aacgtgtcgt tgccgggtcc agcgcgctgg tggcttcatc
    64801 acacagcaat actttgggat tgctggctaa tgcacgggca atcgccacac gttgtttctg
    64861 cccaccggaa agattcgacg ggtagctatc atgcttatcg ccaagaccaa ccaatgacag
    64921 caattccgtc acgcgacgtt tgatctcgtc tttcggtgtg ttgtccagct ccagcggcag
    64981 agccacgttg ccaaaaacag tacgcgaaga gagcaggtta aaatgctgga aaatcatacc
    65041 aatctggcgg cgagctttgg tcaactcgga ttctgacagc gtggtcagtt cctggccatc
    65101 gaccagcacg ctaccctcgg ttgggcgctc cagcaggttt acacaacgta taagcgtact
    65161 cttacccgcg cctgaggcac cgataacgcc ataaatttgt ccagctggca catgcaggct
    65221 gacgttgttc aacgcctgga tgatgcgggt gccctggtgg aacactttgg tgatattcga
    65281 aagttttatc attgattatt tattatcgtc attaagttag tcgtggcatc tcgaatgcct
    65341 gaaacgggca acgccgtcaa tgaaatggat gttaaggcat ccagacgtct aaatcaatca
    65401 ggtttatgcg aagagcactt tcttgcaggt cgaaacatgc gatactagcg tcacatgcct
    65461 tattaaggag ctataaaagg tggcaaagag cgtacccgca atttttcttg accgtgatgg
    65521 caccattaat gtcgatcacg gctatgtcca tgagatcgac aactttgaat ttatcgacgg
    65581 tgttattgac gccatgcgcg agctaaaaaa aatgggcttt gtgctggtgg tagtaaccaa
    65641 ccagtctggc attgctcgcg gtaaatttac cgaagcacag tttgaaacgc tgaccgagtg
    65701 gatggactgg tcgctggcgg accgagatgt cgatctggat ggtatctatt attgcccgca
    65761 tcatccgcag ggtagtgttg aagagtttcg ccaggtctgc gactgccgta aaccacatcc
    65821 ggggatgctt ttgtcagcac gcgattattt gcatattgat atggccgctt cttatatggt
    65881 gggcgataaa ttagaagata tgcaggcagc ggctgcggcg aacgtgggaa caaaagtgct
    65941 ggtgcgtacg ggtaaaccta ttacgcctga agcagaaaac gcggcggatt gggtgttaaa
    66001 tagcctggca gacctgccgc aagcgataaa aaagcagcaa aaaccggcat aatgattaaa
    66061 agatgagcga ttgaaataaa aatgcatttt tccacttgtc ttcctgagac gactccctat
    66121 aatgcgcctc catcgacacg gcggatgtga atcacttcac acaaacagcc ggttcggttg
    66181 aagagaaaaa tcctgaaatt cagggttgac tctgaaagag gaaagcgtaa tatacgccac
    66241 ctcgcgacag tgcgctaaag tgcgtcgcaa ctgctcttta acaatttatc aggcaatctg
    66301 tgtgggcact cgaagatacg gattcttaac gtcgcaaggc gaaaaatg
//
LOCUS       DAJVUJ010000006        64455 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.006, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000006 DAJVUJ010000000
VERSION     DAJVUJ010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 64455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64455
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.006"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..96
                     /locus_tag="OQQ48_000391"
     CDS             <1..96
                     /locus_tag="OQQ48_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCS1951277.1"
                     /translation="RLNAVLDYIDAVAATDTSTAPDVIWPELPEA"
     gene            complement(68..505)
                     /locus_tag="OQQ48_000392"
     CDS             complement(68..505)
                     /locus_tag="OQQ48_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCS1951278.1"
                     /translation="MFYSPSLNIFVNPALKDDYINANSWPDDALAVSDDVYNEFAINT
                     PPDDKIRVAGKNGLPTWALIPPPSYEELIQQAESERQLLLNQANEYMNSKQWPGKAAI
                     GRLKGEELAQYNLWLDYLDALELIDTSSAPDIEWPTPPAVQAR"
     gene            complement(507..893)
                     /locus_tag="OQQ48_000393"
     CDS             complement(507..893)
                     /locus_tag="OQQ48_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1951279.1"
                     /translation="MPVSHYLCIFLNVGLGELSLAGTASGVIGLNGYVTIPLIISGSR
                     RTLIIQWGQARFGGSGGEDAGYLNDFPFAFPSACYGMIVSHVGHTPSGAGILSASAIT
                     SNQFRGFSSIATAANAVLGRYIAIGV"
     gene            924..1490
                     /locus_tag="OQQ48_000394"
     CDS             924..1490
                     /locus_tag="OQQ48_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016529756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCS1951280.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNALNCAGCERIFEDKISGTKPDRPG
                     LKKLLRTLSAGDTLVVWKLDRLGRSMRHLVTLIEELRQRGVNFRSLTDSIDTSTPMGR
                     FFFHVMGALAEMERELIVERTRAGLAAARAKGRVGGRRPKLTTEQWAQIGRLLEAGES
                     RQRIALIFDVGVSTIYRKFPANESNESP"
     gene            1633..2805
                     /locus_tag="OQQ48_000395"
     CDS             1633..2805
                     /locus_tag="OQQ48_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath protein"
                     /protein_id="HCS1951281.1"
                     /translation="MAQDYHHGVRVVEVNEGTRSITTVSTAIVGMVCTGDDADAKMFP
                     LNKPVLITDVLTASGKAGESGTLARSLDAIADQAKPVTVVVRVPQGETEEETTTNIIG
                     AVTAEGKKTGMKALLSAQSQLGVKPRILGVPGHDTKAVATELLSVAQSLRGFAYLSAY
                     GCKTVQEAITYRENFSQREGMLIWPDFTGWDTVLNAEATAYATARALGLRAKIDEQTG
                     WHKSLSNVGVNGVTGISADVFWDLQDPATDAGLLNQNDITTLIRKDGFRFWGSRCLSD
                     DPLFAFENYTRTAQVLMDTMAEAHMWAVDKPLNPSLARDIIEGIRAKMRSLVSQGYLI
                     GGDCWLDESVNDKDTLKAGKLTIDYDYTPVPPLENLMLRQRITDQYLVNFSSQVSA"
     gene            2815..3330
                     /locus_tag="OQQ48_000396"
     CDS             2815..3330
                     /locus_tag="OQQ48_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HCS1951282.1"
                     /translation="MALPRKLKHLNLFNDGNNWQGIVESLTLPKFTRKYEKYRGGGMP
                     GAVDVDLGLDDSALDTEFSIGGTELLLFKQMGKATVDGIQLRFTGSIQRDDTGEVQAV
                     ELVVRGRHKEVDSGEWKTGESNTTKVTSTNSYAKLTINGEVLYEVDLINMVEIVDGVD
                     LMEAHRNALGL"
     gene            3385..3687
                     /locus_tag="OQQ48_000397"
     CDS             3385..3687
                     /locus_tag="OQQ48_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HCS1951283.1"
                     /translation="MSDKQTEKTIQLDTPIMRGKTEITEIVLRKPQSGALRGTRLQAI
                     MDMDVNAMMTVIPRISSPALTAQEIAEMDPADLTAMSVEVVTFLLKKSVLAGLPTA"
     gene            3702..3821
                     /locus_tag="OQQ48_000398"
     CDS             3702..3821
                     /locus_tag="OQQ48_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HCS1951284.1"
                     /translation="MADIATIFHWPPSVTDVMPLTEVLEWRDKAIQRSGASHE"
     gene            3814..6891
                     /locus_tag="OQQ48_000399"
     CDS             3814..6891
                     /locus_tag="OQQ48_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCS1951285.1"
                     /translation="MSDNNLRLQVILNAVDKLTRPFRSAQASSRELAAAVKKSRDAIK
                     QLDQAGSSLDSFRKLQAENQKLGDRLNYARQRANLLSQELGAMGPPSQRQVVALGRQR
                     LAVQRLEERQKKLQQQTALVRAELYRAGISANDGASATARITRETMRYNRQLSEQEAR
                     LRRVGEQQRKMHAARGAYARRLEVRDRIAGAGATTTAAGLAMGAPVMAAVKSYTSMVD
                     AMKGVAKQVNGLRDDNGNRTARFYEMQGAIKAASEQLPMENGAVDFAALVEGGARMNV
                     ANPDDSWEDQKRDLLAFASTAAKAATAFELPADELSESLGKIAQLYKIPTRNIEQLGD
                     ALNYLDDNAMSKGADIIDVMQRLGGVADRLDYRKAAALGSTFLTLGAAPEVAASAANA
                     MVRELSIATMQSKSFFEGMNLLKLNPEVIEKQMTKDAMGTIQRVLEKVNALPQDKRLS
                     AMTMLFGKEFGDDAAKLANNLPELQRQLKLTAGNDALGSMQKESDINKDSLSAQWLLV
                     KTGAQNTFSSLGETLRQPLMDILYTVKSVTGALRRWVEANPELTGTLMKVAAVVAAVT
                     VGLGTLAVALAAVLGPLAVIRLGFSVLGIKTLPSVTATVTRTSNALSWLAGAPLALLR
                     RGLASSGNAAGLLTAPLSSLRRTASLTGNVLKTVAGAPVALLRSGLSGLRAVAVMFMN
                     PLAVLRGGLAATGTVLRMLASGPLAMLRVALYAVSGLLGALLSLIGLVVTALAGVALV
                     VWKYWQPITAFLGGVVEGFKAAAGPVSAAFEPLKPVFQWIGDKVQALWGWFTDLLTPV
                     KSTSAELQSAAAMGRRFGEALAEGLNMVMHPLDSLKSGVSWLLEKLGIVSKEAAKAKL
                     PESVTRQQPATVNADGKVMMPSGGFPSWGYGFAGMYDSGGYIPRGQFGIVGENGPEIV
                     NGPANVTSRRNTAALAAVVAGMMGVAATPAELPPLHPLALPAKGGEAMVSRAATVPPV
                     QRIEAPMQIIIQTQPGQSAQDIAREVARQLDERERRLKAKARSNYSDQGGYDA"
     gene            6888..7373
                     /locus_tag="OQQ48_000400"
     CDS             6888..7373
                     /locus_tag="OQQ48_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1951286.1"
                     /translation="MMMVLGLYVFMLRTVPYQELQYQRSWRHAANSRVNRRPSTQFLG
                     PDNDMLTLSGVLMPEVTGGRLSLLALEQMAEQGKAWPLIEGSGTIYGMYVIEGLNQTK
                     TEFFRDGMPRRIEFTLSLKRVDESLSDMFGDLSAQLNNLQGTATSALSDISKTVGGLL
                     S"
     gene            7370..8470
                     /locus_tag="OQQ48_000401"
     CDS             7370..8470
                     /locus_tag="OQQ48_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HCS1951287.1"
                     /translation="MNFSSELLNKGNKTPAFSISIEGRDITTVLDNRLMGLTLTDNRG
                     FEADQLDLELDDADGKIVLPRRGAVITLALGWKGQPLFPKGAFTVDEIEHTGAPDRLT
                     IRARSADFRETLNTRREKSWHKTTVGEVVKEIAARHKLKMALGEDLSDKLVEHIDQTN
                     ESDGSFLMRLARQYGAIASVKNGNLLFIRQGQGKSASGKPLPVITITRKDGDSHRFTL
                     ADRGAYTGVIASWLHTREPAKKESTTVKRKRRTKKQKKEPEAKQGDYLVGTDENVLVL
                     NRTYANRSNAERAAKMQWERLQRGVASFSLQLAEGRADLYTEMPVKVSGFKQPLDDAE
                     WTITTLTHTVSPDNGFTTSLELEVRIDDFEME"
     gene            8561..8779
                     /locus_tag="OQQ48_000402"
     CDS             8561..8779
                     /locus_tag="OQQ48_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001504085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HCS1951288.1"
                     /translation="MMICPLCGSAAHTRSSFQVSSLTKERYNQCQNINCSHTFVTHET
                     FVRSIATPKESNPVQPHPMKSGQVALSL"
     gene            complement(9015..10700)
                     /locus_tag="OQQ48_000403"
     CDS             complement(9015..10700)
                     /locus_tag="OQQ48_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HCS1951289.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTAHSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            10970..11347
                     /locus_tag="OQQ48_000404"
     CDS             10970..11347
                     /locus_tag="OQQ48_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HCS1951290.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(11377..11634)
                     /gene="grxA"
                     /locus_tag="OQQ48_000405"
     CDS             complement(11377..11634)
                     /gene="grxA"
                     /locus_tag="OQQ48_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HCS1951291.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            11794..12081
                     /locus_tag="OQQ48_000406"
     CDS             11794..12081
                     /locus_tag="OQQ48_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HCS1951292.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            12065..12787
                     /gene="nfsA"
                     /locus_tag="OQQ48_000407"
     CDS             12065..12787
                     /gene="nfsA"
                     /locus_tag="OQQ48_000407"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HCS1951293.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            12848..13750
                     /gene="rimK"
                     /locus_tag="OQQ48_000408"
     CDS             12848..13750
                     /gene="rimK"
                     /locus_tag="OQQ48_000408"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HCS1951294.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIVHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERYATTEYCLKTGG"
     gene            13838..14314
                     /locus_tag="OQQ48_000409"
     CDS             13838..14314
                     /locus_tag="OQQ48_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HCS1951295.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            14665..15777
                     /gene="potF"
                     /locus_tag="OQQ48_000410"
     CDS             14665..15777
                     /gene="potF"
                     /locus_tag="OQQ48_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HCS1951296.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            15872..17005
                     /gene="potG"
                     /locus_tag="OQQ48_000411"
     CDS             15872..17005
                     /gene="potG"
                     /locus_tag="OQQ48_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HCS1951297.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTQLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVHEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERHEDGLVLYSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            17015..17968
                     /gene="potH"
                     /locus_tag="OQQ48_000412"
     CDS             17015..17968
                     /gene="potH"
                     /locus_tag="OQQ48_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HCS1951298.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVAAAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            17965..18810
                     /gene="potI"
                     /locus_tag="OQQ48_000413"
     CDS             17965..18810
                     /gene="potI"
                     /locus_tag="OQQ48_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HCS1951299.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARHG"
     gene            18870..19358
                     /locus_tag="OQQ48_000414"
     CDS             18870..19358
                     /locus_tag="OQQ48_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HCS1951300.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            19399..20526
                     /gene="rlmC"
                     /locus_tag="OQQ48_000415"
     CDS             19399..20526
                     /gene="rlmC"
                     /locus_tag="OQQ48_000415"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HCS1951301.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGFRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(20701..21432)
                     /gene="artJ"
                     /locus_tag="OQQ48_000416"
     CDS             complement(20701..21432)
                     /gene="artJ"
                     /locus_tag="OQQ48_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HCS1951302.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(21723..22391)
                     /gene="artM"
                     /locus_tag="OQQ48_000417"
     CDS             complement(21723..22391)
                     /gene="artM"
                     /locus_tag="OQQ48_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HCS1951303.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(22391..23107)
                     /gene="artQ"
                     /locus_tag="OQQ48_000418"
     CDS             complement(22391..23107)
                     /gene="artQ"
                     /locus_tag="OQQ48_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HCS1951304.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESEKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(23114..23845)
                     /gene="artJ"
                     /locus_tag="OQQ48_000419"
     CDS             complement(23114..23845)
                     /gene="artJ"
                     /locus_tag="OQQ48_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCS1951305.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(23863..24591)
                     /gene="artP"
                     /locus_tag="OQQ48_000420"
     CDS             complement(23863..24591)
                     /gene="artP"
                     /locus_tag="OQQ48_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HCS1951306.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(24809..25324)
                     /locus_tag="OQQ48_000421"
     CDS             complement(24809..25324)
                     /locus_tag="OQQ48_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCS1951307.1"
                     /translation="MRYSKLTMLIPCALLLSACTTVTPAYKDNGTRSGPCVEGGPGNV
                     AQQFYDYRILHRSNDITALRPYLSDKLATLLSDASRDNNHRELLTNDPFSSRTTLPES
                     ARVASASTIPNRDARNIPLRVDLKQGDQSWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            25450..25773
                     /locus_tag="OQQ48_000422"
     CDS             25450..25773
                     /locus_tag="OQQ48_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HCS1951308.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            25770..26600
                     /gene="amiD"
                     /locus_tag="OQQ48_000423"
     CDS             25770..26600
                     /gene="amiD"
                     /locus_tag="OQQ48_000423"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HCS1951309.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(26597..27607)
                     /locus_tag="OQQ48_000424"
     CDS             complement(26597..27607)
                     /locus_tag="OQQ48_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCS1951310.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMFSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLCSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     FRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGKL
                     PR"
     gene            complement(27706..29136)
                     /locus_tag="OQQ48_000425"
     CDS             complement(27706..29136)
                     /locus_tag="OQQ48_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HCS1951311.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKAPGLGRLCFSLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(29147..30148)
                     /gene="ltaE"
                     /locus_tag="OQQ48_000426"
     CDS             complement(29147..30148)
                     /gene="ltaE"
                     /locus_tag="OQQ48_000426"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HCS1951312.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKVRNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(30185..31903)
                     /gene="poxB"
                     /locus_tag="OQQ48_000427"
     CDS             complement(30185..31903)
                     /gene="poxB"
                     /locus_tag="OQQ48_000427"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HCS1951313.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIVVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(32036..33004)
                     /gene="hcr"
                     /locus_tag="OQQ48_000428"
     CDS             complement(32036..33004)
                     /gene="hcr"
                     /locus_tag="OQQ48_000428"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HCS1951314.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVHTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAKAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(33016..34668)
                     /gene="hcp"
                     /locus_tag="OQQ48_000429"
                     /gene_synonym="priS"
     CDS             complement(33016..34668)
                     /gene="hcp"
                     /locus_tag="OQQ48_000429"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HCS1951315.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVSAYDDRIWTRS
                     IVGWPSVRHLDGDDFSAVITQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATIVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(34812..35711)
                     /gene="lysO"
                     /locus_tag="OQQ48_000430"
     CDS             complement(34812..35711)
                     /gene="lysO"
                     /locus_tag="OQQ48_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HCS1951316.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(36206..36901)
                     /gene="aqpZ"
                     /locus_tag="OQQ48_000431"
     CDS             complement(36206..36901)
                     /gene="aqpZ"
                     /locus_tag="OQQ48_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HCS1951317.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATAKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
     gene            37327..38985
                     /locus_tag="OQQ48_000432"
     CDS             37327..38985
                     /locus_tag="OQQ48_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HCS1951318.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAIEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(38982..39938)
                     /locus_tag="OQQ48_000433"
     CDS             complement(38982..39938)
                     /locus_tag="OQQ48_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF535 domain-containing protein"
                     /protein_id="HCS1951319.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLKLTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            40089..41204
                     /gene="macA"
                     /locus_tag="OQQ48_000434"
     CDS             40089..41204
                     /gene="macA"
                     /locus_tag="OQQ48_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HCS1951320.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAEIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            41201..43147
                     /gene="macB"
                     /locus_tag="OQQ48_000435"
     CDS             41201..43147
                     /gene="macB"
                     /locus_tag="OQQ48_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HCS1951321.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGITLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHMVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALVRE"
     gene            complement(43220..43444)
                     /gene="cspD"
                     /locus_tag="OQQ48_000436"
     CDS             complement(43220..43444)
                     /gene="cspD"
                     /locus_tag="OQQ48_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HCS1951322.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            43767..44087
                     /gene="clpS"
                     /locus_tag="OQQ48_000437"
     CDS             43767..44087
                     /gene="clpS"
                     /locus_tag="OQQ48_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HCS1951323.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            44118..46394
                     /gene="clpA"
                     /locus_tag="OQQ48_000438"
     CDS             44118..46394
                     /gene="clpA"
                     /locus_tag="OQQ48_000438"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HCS1951324.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(46738..46825)
                     /locus_tag="OQQ48_000439"
     tRNA            complement(46738..46825)
                     /locus_tag="OQQ48_000439"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(46789..46791),aa:Ser,seq:gga)
     gene            complement(47079..47297)
                     /gene="infA"
                     /locus_tag="OQQ48_000440"
     CDS             complement(47079..47297)
                     /gene="infA"
                     /locus_tag="OQQ48_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCS1951325.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(47582..48286)
                     /gene="aat"
                     /locus_tag="OQQ48_000441"
     CDS             complement(47582..48286)
                     /gene="aat"
                     /locus_tag="OQQ48_000441"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HCS1951326.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(48328..50049)
                     /gene="cydC"
                     /locus_tag="OQQ48_000442"
     CDS             complement(48328..50049)
                     /gene="cydC"
                     /locus_tag="OQQ48_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HCS1951327.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMMVTIGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAESL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(50050..51816)
                     /gene="cydD"
                     /locus_tag="OQQ48_000443"
     CDS             complement(50050..51816)
                     /gene="cydD"
                     /locus_tag="OQQ48_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HCS1951328.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQCGEVELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEHELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(51939..52904)
                     /gene="trxB"
                     /locus_tag="OQQ48_000444"
     CDS             complement(51939..52904)
                     /gene="trxB"
                     /locus_tag="OQQ48_000444"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCS1951329.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            53449..53943
                     /gene="lrp"
                     /locus_tag="OQQ48_000445"
     CDS             53449..53943
                     /gene="lrp"
                     /locus_tag="OQQ48_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HCS1951330.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            54078..58106
                     /locus_tag="OQQ48_000446"
     CDS             54078..58106
                     /locus_tag="OQQ48_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCS1951331.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDEE
                     EIIYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVTSVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQPQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPVQQPYYAPAAEQPVQQPYYATAPEQPAQQPYYAPA
                     PEQPVAGNAWQAEEQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPV
                     VEETKPARPPLYYFEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSAAAVP
                     PVEAAAAVSPLASGVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKR
                     IRVPTRRELASYGIKLPSQRAAEEKAREARRNQYDSGDQYNDDEIDAMQQDELARQFA
                     QTQQQRYGEQYQHDVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDF
                     EFSPMKALLDDGPHEPLFTPIVEPVQQPQQLVAPQQQYQQPQQPVAPQQQYQQPQQPV
                     APQPQYQQPQQQVAPQPQYQQPQQPVAPQQQDTLLHPLLMRNGDSRPLHKPTTPLPSL
                     DLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGV
                     KAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRD
                     NPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDV
                     RFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNL
                     AGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKK
                     VEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILD
                     QAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSD
                     SESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEA
                     QGIVSEQGHNGNREVLAPPPFD"
     gene            58261..58872
                     /gene="lolA"
                     /locus_tag="OQQ48_000447"
     CDS             58261..58872
                     /gene="lolA"
                     /locus_tag="OQQ48_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HCS1951332.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            58883..60226
                     /gene="rarA"
                     /locus_tag="OQQ48_000448"
                     /gene_synonym="mgsA"
     CDS             58883..60226
                     /gene="rarA"
                     /locus_tag="OQQ48_000448"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HCS1951333.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVDDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPREIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            60317..61609
                     /gene="serS"
                     /locus_tag="OQQ48_000449"
     CDS             60317..61609
                     /gene="serS"
                     /locus_tag="OQQ48_000449"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCS1951334.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            61848..64292
                     /gene="dmsA"
                     /locus_tag="OQQ48_000450"
                     /pseudo
     CDS             61848..64292
                     /gene="dmsA"
                     /locus_tag="OQQ48_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
     gene            64303..>64455
                     /locus_tag="OQQ48_000451"
     CDS             64303..>64455
                     /locus_tag="OQQ48_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCS1951335.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDN"
BASE COUNT        15852 a        16182 c        17363 g        15058 t
ORIGIN      
        1 cgacttaacg ccgtgttgga ttacattgac gcggtggcgg caacagatac cagcaccgcg
       61 ccggatgtca tctggcctga actgccggag gcgtaggcca ttcaatatct ggagcactgg
      121 aggtatcaat cagttccagt gcgtccagat aatccagcca caaattatat tgcgccagtt
      181 cctcaccttt cagacgacca atagcggctt taccgggcca ttgtttactg ttcatgtatt
      241 cgttggcctg attaagcaat aattgccttt ctgattctgc ctgttgaata agttcttcat
      301 atgatggtgg aggtattagt gcccatgtgg gtaatccatt ttttcctgca acacgaattt
      361 tgtcatctgg gggcgtattg attgcaaatt cattataaac atcatcactg acagccagag
      421 catcatctgg ccatgaattt gcattaatgt aatcatcctt aagcgcagga ttcacaaaaa
      481 tgtttaaaga tggactataa aacatattaa acccctatag cgatataacg acctaataca
      541 gcatttgcag cagtcgctat gcttgaaaaa ccgcggaact gatttgatgt aattgcagaa
      601 gccgacagga ttcctgcgcc tgaaggtgta tgccccacat gactaactat catcccataa
      661 catgctgacg gaaaggcaaa aggaaaatca ttaagatatc ctgcatcttc accaccagac
      721 ccaccaaatc tcgcctgccc ccactgaata atcagtgttc tccgggaacc tgaaataatt
      781 aacggaatcg ttacataccc attcaggcca atgacacccg atgcagtgcc agccagagat
      841 aattctccca aaccaacgtt taagaaaatg cagagataat ggctaactgg catcatcccc
      901 ggtttttatt caggggattg atcatgctta ttggctatgt acgcgtgtca acaaatgacc
      961 agaacaccga tttgcaacgt aatgcactga actgcgcggg atgtgagcgg atttttgagg
     1021 ataaaatcag tggcactaag cccgacagac cggggcttaa aaaactgctc aggacactat
     1081 cggcaggaga cacgctggtt gtctggaagc tggacagact gggacgcagt atgcggcatc
     1141 ttgttacgct gatagaagag ttgcgccagc gtggtgtgaa tttccgaagc ctgactgaca
     1201 gtattgatac cagtacccca atgggccgtt tcttttttca cgtcatgggg gctctggctg
     1261 aaatggaacg tgaactgata gttgaacgta ccagagcagg gctggctgca gctcgtgcca
     1321 aaggcagagt aggtggacgc cgtcctaagt tgaccaccga acagtgggca cagattggac
     1381 gtttactcga ggccggagaa tcaagacagc gtattgcact gatttttgat gtgggcgttt
     1441 ctaccattta tagaaaattt ccggcgaatg agagcaatga atcgccctga gtcagtatta
     1501 ttttgatttt tcctgtaagc ggacaaatac cgtcattttg tgtgaataac gacacaactg
     1561 cgcttagctg tttgtcaggc acaatcactt caacataggg cgaagcctaa tccaatcagg
     1621 aggttcgcca ctatggctca ggattaccac cacggggtgc gcgttgttga agtcaacgaa
     1681 ggcacccgat ccattaccac ggtgagcacc gccatcgtgg gtatggtctg cacgggcgat
     1741 gatgccgatg caaaaatgtt tcctcttaat aaacctgtgc tgatcactga tgtgctgacc
     1801 gccagcggta aagcgggtga gtctggcacg ctggcccgtt cgctggatgc catcgctgac
     1861 caggcaaaac ccgtgaccgt tgttgtgcgt gtgccgcagg gtgaaacgga agaagaaacc
     1921 acaaccaata tcattggcgc agtgaccgcc gagggtaaaa aaacaggtat gaaagccctg
     1981 ttatctgccc agtcacagct cggcgttaaa ccgcgcattc tcggcgtgcc aggtcacgac
     2041 acgaaggcgg tagctactga gttgctgagc gtggcgcaaa gcctgcgtgg atttgcttac
     2101 ctgtcagcgt atggttgcaa gacggtgcag gaagcaatca cttaccgtga aaacttcagt
     2161 cagcgtgaag gaatgctgat ctggcccgac tttactggct gggacacggt gctgaatgcc
     2221 gaagcaacgg cttatgccac cgcccgtgcg ctcggtctgc gcgctaaaat tgatgagcag
     2281 accggatggc acaaaagcct gtccaacgtg ggcgtgaacg gtgtcaccgg aatttctgct
     2341 gatgtgttct gggatctgca ggacccggca accgatgcag gtctgcttaa ccagaacgat
     2401 atcaccacgc ttatccgcaa agacggtttc cgcttctggg gttcccgctg tctgagcgat
     2461 gatccgctct ttgccttcga aaactacacc cgcacggcgc aggtgctgat ggacacgatg
     2521 gcagaagcgc acatgtgggc ggtggacaaa ccgctgaacc cgtcgctggc ccgcgacatt
     2581 atcgaaggta tccgcgccaa aatgcgtagc ctggtcagtc agggctatct cattggtggt
     2641 gattgctggc tggacgagtc ggtgaacgat aaagacacgc tgaaagccgg gaaactcacc
     2701 atcgattacg actacacgcc agtgccgcca cttgaaaacc tgatgctgcg tcagcgcatc
     2761 accgatcagt acctggtgaa tttctccagc caggtcagcg cgtaagggga caacatggct
     2821 ttaccacgca aattaaaaca cctgaacctg tttaacgacg ggaacaactg gcaggggatc
     2881 gttgagtcgc tgacgctgcc gaaattcacc cgcaaatatg agaagtatcg cggcggcggt
     2941 atgcccggag cggtggatgt ggatctggga ctggatgaca gtgcactgga tacagaattt
     3001 tccattggtg gtactgaact gctgctgttt aagcagatgg gcaaagccac ggtggatggc
     3061 atccagctgc gctttaccgg ctctattcag cgtgatgata ccggggaagt gcaggccgtg
     3121 gagcttgtgg tgcgtggacg tcacaaagaa gtagattccg gcgagtggaa gacgggcgaa
     3181 agcaacacta ccaaagtgac cagtaccaac agctacgcga agctgaccat caatggtgag
     3241 gtgctctatg aagtggacct gatcaacatg gtggaaattg tggacggtgt ggacctgatg
     3301 gaagcgcacc gcaacgccct cggcctctga tgtatctgaa cggcgcggaa tgccgcgcca
     3361 gaacctaatt tacaggacaa caaaatgagc gataagcaga ctgaaaagac cattcaactg
     3421 gatactccca tcatgcgcgg taaaacagaa attaccgaaa ttgtgttgcg taaaccgcag
     3481 tccggtgcgc tgcgcggcac acgcctgcag gccattatgg atatggatgt gaacgcgatg
     3541 atgaccgtga tcccccgcat ctccagtccg gcactgactg cacaggaaat tgcagagatg
     3601 gacccggcag atctcactgc catgtcggtt gaggttgtca cttttttgtt gaagaagtcg
     3661 gtgcttgccg gtttaccgac agcctgacgg ttgacgatct ggtggctgat atcgccacca
     3721 tctttcactg gccgccatcc gttactgacg ttatgccgct gaccgaagtg ctggaatggc
     3781 gggataaagc gattcagaga agcggggcca gccatgagtg acaataacct gcgtctgcag
     3841 gtgattctta atgcggttga caaactcacc cgcccatttc gatccgcgca ggccagttca
     3901 agagaactgg ctgctgctgt caaaaaatcc cgcgatgcaa taaagcagct tgatcaggcc
     3961 gggagcagtc tggacagctt ccgaaagctg caggcagaaa atcagaaatt aggcgacagg
     4021 ctgaactatg cccgccagcg tgcaaatttg ctcagtcagg aactgggagc gatggggccg
     4081 ccttcgcaac gtcaggttgt tgctctgggc cgtcaacggc tggctgttca gcgcctagaa
     4141 gaacgccaga aaaagctgca gcagcagacg gcgcttgtgc gtgcagaact ttatcgtgct
     4201 ggtatttcag ctaatgatgg cgccagtgcg acggcccgca ttacccgtga aacgatgcgt
     4261 tataacaggc agctttctga gcaggaagcg aggttacgac gtgtcgggga gcaacagcga
     4321 aaaatgcacg ccgcccgggg ggcttacgcc aggcgtcttg aggtaaggga tcgtattgca
     4381 ggtgccggag ccaccaccac ggctgcaggg ctggcaatgg gcgcaccagt gatggcggca
     4441 gtaaaaagct ataccagcat ggtagatgcc atgaaaggtg tggcaaagca ggtcaatggt
     4501 ctgcgtgacg ataatggcaa ccgcactgcg cgtttttacg aaatgcaggg tgccatcaag
     4561 gctgccagcg aacagttgcc gatggaaaac ggtgctgtgg acttcgccgc actggttgaa
     4621 ggtggtgcgc gcatgaacgt cgcaaaccct gacgacagct gggaagacca gaaacgtgac
     4681 ctgctggcct tcgccagcac ggcggcaaag gcggcaacag cctttgaact gccagcggat
     4741 gaactgtcag aaagtctggg gaaaatcgcc cagctctaca aaatccctac ccgcaatatt
     4801 gagcagctcg gtgatgcgct gaactatctg gatgataacg ccatgtcgaa aggggcggac
     4861 atcattgatg tcatgcaacg cctgggcggt gtggctgacc gtctggatta tcgtaaagcg
     4921 gcggcgctgg gttccacctt cctgacactc ggcgctgcgc ctgaggttgc agccagtgca
     4981 gcaaacgcga tggtgcgtga attgtccatt gccaccatgc aaagcaagag tttctttgaa
     5041 gggatgaatc tgctgaaact caatccagaa gtgattgaaa agcagatgac gaaggatgcg
     5101 atgggaacta tccagcgggt gctggagaag gtgaatgcgc tgccacagga taagcgcctg
     5161 tctgccatga ccatgttgtt tggtaaagag tttggcgatg acgcagcgaa actggcaaac
     5221 aacctgccgg aacttcagcg ccagttaaaa ctgactgcgg gcaatgatgc gctcggttcc
     5281 atgcagaaag aatccgacat caacaaggat tcactttctg ctcagtggtt gctggtcaaa
     5341 accggagcac agaacacctt cagcagcctg ggggaaacgc tgcgccagcc gctgatggat
     5401 attctgtaca cggtgaaaag cgtcacgggg gcgttgcgtc gctgggtgga agctaacccg
     5461 gaactgacgg gcactctgat gaaagtagcc gctgtggtag ctgccgttac cgtgggcctc
     5521 ggcaccttag cggtggcgct ggctgcagtg ctggggccgc tggcagtcat ccgtctggga
     5581 ttctctgtgc tgggtatcaa aacgttacct tccgttacgg caacagtaac ccgaaccagc
     5641 aacgcgttgt cctggctggc tggcgcacca ctggcactgc tgcgacgcgg gcttgcttca
     5701 tcgggcaacg ctgcaggttt acttactgcg ccgttgtcgt ctttgcgccg cacggcatca
     5761 ctgacgggaa atgtcctgaa aactgtagca ggtgcgccgg ttgcactttt gcggtctgga
     5821 ttatccggtt tacgtgcggt tgctgtgatg tttatgaatc cactggcggt actacgcggc
     5881 ggactggctg ccacaggcac ggtgctgcgt atgctcgcat ccggtccact ggcgatgctg
     5941 cgcgttgccc tgtatgccgt ctctggtctg ttaggtgctc tgctcagtct gataggtctt
     6001 gtggttactg cactggcggg tgtggcgctg gttgtctgga aatactggca gcccatcacc
     6061 gcatttctcg gtggcgtggt ggaaggattc aaagcggcgg caggtcctgt cagtgccgcg
     6121 ttcgaaccgc ttaagcctgt gttccagtgg attggcgaca aagtacaggc gctgtggggc
     6181 tggtttactg atctgctgac gcccgttaag tcgacctctg ccgaactgca gagtgcagcg
     6241 gcaatggggc gacgattcgg ggaggctctg gcggaagggc tgaatatggt catgcatccg
     6301 ctggactccc tgaaatccgg cgtttcctgg ttgctggaga aacttggcat tgtcagtaaa
     6361 gaggccgcaa aggcgaaact gccggaaagc gtgacgcgtc agcaacctgc gacggtgaat
     6421 gcagacggta aagtgatgat gccatcgggt ggttttccgt catggggata tggctttgcg
     6481 gggatgtatg acagcggcgg gtatatcccg cgcgggcagt ttggcatcgt cggtgaaaac
     6541 gggccggaaa ttgttaacgg cccggcaaat gtgaccagcc ggagaaatac agctgcactg
     6601 gctgccgttg ttgccggaat gatgggtgtt gctgctacgc cagcagagct tccaccgttg
     6661 caccctttgg cacttcccgc gaaaggcggt gaagcgatgg tgagtcgtgc agccactgtg
     6721 ccgcccgttc aacggattga ggcaccgatg cagatcatca ttcaaacgca gccaggacaa
     6781 agtgcgcagg atattgcgcg ggaggtggcc cgccagcttg atgaacgtga acgcaggctg
     6841 aaggcaaaag ccaggagtaa ctacagcgat caggggggat acgacgcatg atgatggtgc
     6901 tgggattgta cgtgtttatg ctgcgcaccg tgccgtatca ggagctgcag tatcaacgca
     6961 gctggcgaca tgcggcaaac agccgggtaa accgacgccc gtcaacgcag tttctgggac
     7021 cggacaacga catgctgacg ctttctggtg ttcttatgcc ggaggtaacg ggcggcaggc
     7081 tgtcgttgct ggctctggag cagatggcag aacagggaaa agcatggccc ctgattgaag
     7141 gcagcggcac gatttacggc atgtatgtga ttgagggact gaatcagact aaaacggagt
     7201 ttttccgcga cggtatgccg cgccggattg agttcaccct gtcgctaaaa cgcgtggatg
     7261 aatccctgtc cgatatgttc ggtgatctca gtgcgcaact gaataatctg cagggaacgg
     7321 caacatctgc cttaagcgat atcagtaaaa cggtgggagg gctgctgtcg tgaatttcag
     7381 ctctgaactg cttaacaaag gcaacaaaac tccggcattc agcatcagta ttgaaggcag
     7441 ggatatcacc actgtgctgg acaaccgcct gatggggctg acgctgacgg ataaccgggg
     7501 ctttgaagcg gaccagcttg atctggagct ggacgacgcc gatggaaaaa tcgtgctgcc
     7561 gcgccgtggg gctgtcatta cgctggcgct gggctggaag ggacagccgc ttttcccgaa
     7621 aggggcattc acggtggacg agattgaaca cactggcgca ccggatcgcc tgactatccg
     7681 ggcgcgaagt gctgattttc gtgaaacgct gaatacccgc cgtgaaaagt cgtggcacaa
     7741 gaccaccgtt ggggaagtgg tgaaggaaat agctgcgcgg cacaaactga agatggcatt
     7801 gggtgaagac ttgtcggata aactcgtgga gcatatagac cagactaatg agagtgacgg
     7861 cagttttttg atgcggctgg cgcgccagta cggtgctatt gcgtcggtga aaaatggcaa
     7921 tctgttattc atccggcagg ggcagggtaa aagcgccagc ggtaaaccac taccggtgat
     7981 cactatcaca cgcaaggacg gcgacagtca ccgctttacc ctggcagatc gcggagctta
     8041 cacgggcgtc attgccagct ggttgcatac ccgcgaaccc gcgaagaaag aaagcaccac
     8101 ggtgaagcgt aagcgcagga ctaagaagca gaagaaagag ccggaagcga agcagggcga
     8161 ttacctggtg ggtacggatg aaaacgtgct ggtacttaat cgcacttatg ccaaccggag
     8221 caacgccgaa cgggcagcga aaatgcagtg ggaacgcctg caacgcggcg ttgcatcatt
     8281 ctcgctacaa ctggcggaag ggcgggcaga tctctacaca gaaatgcctg tgaaggtcag
     8341 tggctttaaa cagccgctag atgatgcgga atggaccatt acgactctga cgcatactgt
     8401 cagcccggat aacggtttta cgaccagtct ggagcttgaa gtgaggattg atgatttcga
     8461 aatggaatga ttcttcgcaa tggagaactt ttaagtttgc aaaatggaat aatgcggtat
     8521 cattattgtg aatttagcaa aaatggggag aactcgaaaa atgatgattt gcccactgtg
     8581 tggaagtgcc gcccatactc gcagcagttt tcaggtatct tcattgacca aagagcgtta
     8641 caaccagtgc cagaacatta actgcagcca tacttttgtt acccatgaaa cttttgttcg
     8701 ttcgattgca acgccaaaag agtcaaatcc ggttcagccg catccaatga aatcaggaca
     8761 ggtggcgctc tctctttgac gctgccgcca ttttgtcgcc atcgttaaaa aacagtgctt
     8821 ctaacatcat gattttaaaa ggcataaatt tcagacaaca aaaaacccat caaccttgaa
     8881 ccgaaatggc ggggttgatg ggctccacaa aatggggaca tcaaagaaaa gcagtggcac
     8941 taattaagac tgatgccctg cggaaaagtt ctgcggttgt gcaaaaaaat ttcattttca
     9001 gggcaacttc agttttatcc taatcctggc cataccatga cgatgattgt ccctgccagc
     9061 gtcagcagga cgttggcgat tgcgtaggtg cccgcatagc ccagcgcagg gatgttactg
     9121 tgagctgtat cactgatgat ctccattgcc ggcgcgcagg tgcgtgcgcc catcattgcg
     9181 ccgaacaaca gtgcgcggtt cattcgcaat acataagcac cgaacaagaa acagataacc
     9241 acgggcacaa gactgacaat taatccggca atcaacatct gaccgccaat cgcgcccagg
     9301 ccgttattaa taccgctacc ggcgctcaga ccaacgcctg ccataaacac catcaagccg
     9361 aactctttca ccatgcttaa tgcaccttgc ggaatgtaac cgaaggtcgg gtggttagca
     9421 cgcataaagc ccagcataat tccggcgaat aacaacccgg cagcgttccc catgccgaaa
     9481 ctgaatgtgc tgaactggaa ggtgatcatc ccgatcatca gcccaataac aaagaaggcg
     9541 caaaatgcca gcaggtcagt gacctggctg tgaatcgaga taaagccgat gcgatcggcg
     9601 atggttttta cgcgacgggc atcaccgctg acttgtaaaa cgtcaccttt gttaagcacg
     9661 acgttgtcat ctatcggcat ctcaatctgg ctacgaatga cgcggttaag gaagcaaccg
     9721 tgatcggtca acttcagttg tgcgagacgt ttacctacag cgttatggtt tttaacgacc
     9781 acttcttcag tgacgatacg catgtcgaga aggtcacgat cgaaaacttc tttaccgtta
     9841 cggaagctgg gatcgagtcg ggcatgggcg tcgggatagc ctaccaacgc tatttcatcg
     9901 cccatttgta gcacggcatc accgtctgga tttgccagaa tcccgttacg tcgaatacgt
     9961 tcaatgtagc agccggtttg tcgataaata cccagttcac gcagattttt gccgtcggtc
    10021 caggccacca gctccgggcc gacgcgatag gcgcggatca ccggtaaata aaccttacgg
    10081 ttggcatcag tgtccaggcc acgttcgcgg gcgatttgct gggcgctggt ctgtaagtcc
    10141 tgatgctgca atttcggcaa gtaacgcgca ccaacaatca aactcaccag accgattaaa
    10201 taggttaagg catacccgag gctcagatta tccagtgcca gtgagagctg cctgctttcc
    10261 atgccggaat gacgcagtgt atcgccagca ccgaccagaa ccggtgtcga cgtcatagag
    10321 cctgctaaca taccggccgt caggccaata tcccagccaa acagcttacc taaccctaag
    10381 gcgatcacca gcgcactgcc aaccatcacc agtgctaaca ttaggtaatt tttcccatcg
    10441 cgaaaaaaaa tggaaaaaaa gttcggtccg gcttcgaccc cgacgcagaa aataaacagc
    10501 ataaagccaa gattaagcgc atcggtgtta atgctgaaat gttgttggcc taataacagc
    10561 gatacgacta aaacgccaat ggaattaccc agttggatcg aaccaagtcg taactttccg
    10621 agacatagcc caagcgcgag gaccacaaat aataacagaa tgtaattccc atttaacaat
    10681 tcggcgacgt ttatattcac ggaggctaac ttcttgttta ctagtaagct gttgaaagaa
    10741 atggtaattt acgataatgt tttttaccag aattcagggc gcagattcat tcagcgcacc
    10801 taaacgatag taaagtaaca atatatttta ctagtgtaat cacattaggt atcaacggct
    10861 atatgaattg cgttggccta tattagcatg gaatgcgaag cggctttatc ttactgaacg
    10921 ccacactggc gaaaaatgtg ttcgatagac gcagtgtcag gaggaacgag tgaaacataa
    10981 acaacgttgg gcgggggcaa tctgctgttt tgtcctcttc attgtggtgt gcctttttct
    11041 ggcgacgcac atgaaaggcg cttttcgggc tgccgggcat cctgaaatcg gcttgctgtt
    11101 tttcattctt cctggagcag ttgccagttt cttttcacag cgtagagaag tcctgaaacc
    11161 tctatttggc gcaatgctgg cggcaccctg ttcaatgctc attatgcggc tgtttttttc
    11221 accgacgcgc tcattctggc aagagctggc atggttacta agcgcggtgt tctggtgtgc
    11281 gctgggggca ctgtgtttct tatttatcag tagtttgttt aaaccacagc acagaaaaaa
    11341 tcagtaaagc cctcaacgcg agggcttgtc agacgatcag gcgtccagat tttctttcac
    11401 ccatgcagca aaatcggtat agccgccgat atgttgctga tcgacaaaaa tctgcggcac
    11461 ggtttctacg ggtttacctg ccttttgttg tagatcttct ttagtgatcc cttccgcacg
    11521 aatatctaca tactgatact gaaaatcatc gcgttcattg ctcaatttct cagccagatc
    11581 ttttgcacgc acacagtaag ggcaacccga acgaccaaaa ataacggttt gcattatttc
    11641 tctcctcata gatttatgcc tgtaatgatc acgctaaaat gtattcgctg aaagtaggtt
    11701 taacctgttg cattaattgc taaaagctat aactgttaaa cacaatacag tgaaaagttt
    11761 tagactgaag gctcactttg cagagggaag cgtatgcgcg cgatcggtaa attgcctaaa
    11821 ggcgtgttga tactggaatt tatcggaatg atgctactgg cggtggcgct gctgtcggta
    11881 agcgactccc tgtcgctgcc tgagccattt tctcggccag aagtgcagat tctgatgatt
    11941 tttctcggtg ttttgctcat gcttcccgct gcggtggtgg ttattcttca ggtggcaaaa
    12001 cgtcttgccc cacagctgat gaaccgtcca ccgcaatatt cacgttcaga aagagaaaaa
    12061 gataatgacg ccaaccattg aacttatttg tggccatcgc tccattcgcc atttcactga
    12121 tgaacccatt tccgaagcgc agtgtgaggc gattattaac agcgcccgtg cgacgtccag
    12181 ttccagtttt ttgcagtgta gtagcattat tcgcattacc gacaaagcat tacgtgaaga
    12241 actggtgacg ctgaccggcg ggcaaaaaca cgtagcgcaa gcggcggagt tctgggtgtt
    12301 ctgtgccgac tttaaccgcc atttacagat ctgtccggat gctcagctcg gcctggcgga
    12361 acaactgttg ctcggtgtcg ttgatacggc aatgatggcg cagaatgcat taaccgcagc
    12421 ggaatcgctg ggattgggcg gggtatatat cggcggcctg cgcaataata ttgaagcggt
    12481 gacgaaactg ctgaaattac cgcagcatgt tctgccgctg tttgggctgt gccttggctg
    12541 gcctgcggat aatccggatc tcaagccgcg tttaccggcc tccattttgg tgcatgaaaa
    12601 cagctatcaa ccgctggata aagacgcact ggcgcagtat gacgagcaac tggcggaata
    12661 ttacctcacc cgtggcagca ataatcgccg ggatacctgg agcgatcata tccgccgaac
    12721 aatcattaaa gaaagccgcc catttattct ggattatttg cacaaacagg gttgggcgac
    12781 gcgctaaaac cgccacgtcg atgtatgata cgcgggcttt tgaccaggtc tgacagagag
    12841 gtgcagggtg aaaattgcca tattgtcccg ggatggaacg ctctattcgt gtaagcggct
    12901 gcgtgaagcc gctatacagc gcggtcacct ggttgaaatt cttgatccgc tttcttgcta
    12961 catgaacata aatcctgcgg cgtcttctat tcactacaaa ggccgcaagt taccccattt
    13021 tgacgcagtg atcccgcgta ttggcaccgc cattaccttt tatgggacgg cggcactgcg
    13081 ccagttcgag atgctgggga gctatccgct caatgagtcg gtcgccattg cccgggcgcg
    13141 tgacaaattg cgttccatgc aactgctggc gcgtcagggc atcgacctgc ctgtcacggg
    13201 cattgtgcat tcgccggatg ataccagcga tttaatcgac atggtcggtg gtgcgccgct
    13261 ggtggtcaag ttggttgaag gcacgcaggg aattggcgtc gtgctggcgg agacgcgtca
    13321 ggcggcggaa agcgtgattg acgctttccg cggtctgaac gcgcatattc tggtgcagga
    13381 atatatcaaa gaggcgcaag ggtgcgatat ccgctgtctg gttgttggcg atgaagtggt
    13441 cgctgcgatt gaacggcggg cgaaagaggg cgattttcgt tccaatttgc atcgtggcgg
    13501 cgcggcaagc gtcgccagta tcacaccaca ggagcgtgaa atcgcgataa aagccgcgcg
    13561 aacgatggcg ctggacgttg ctggtgtgga tattctgcgt gctaatcgcg ggccgttggt
    13621 gatggaagtg aatgcgtcgc cggggctgga aggaatagaa aaaaccactg gtatcgacat
    13681 cgcgggtaaa atgatccgct ggatcgaacg ctacgctacg acagaatatt gcctgaaaac
    13741 gggtggttag tcgcaatcac attactgatc atggttttgc ctgcgctttt tgcgtaagct
    13801 gtgccggtct ttttatcgaa agaggttgta caaaattatg acatcgctgg tcgttcctgg
    13861 tctggatacg ctgcgtcaat ggctcgatga cctggggatg agtttttttg aatgtgataa
    13921 ctgtcaggct ctgcatctgc cccatatgca gaatttcgac ggtgtctttg atgccaaaat
    13981 cgatctgatc gataacacga tcctgttttc tgccatggcg gaagtccgac cttcagccgt
    14041 attgccgctg gcggcggatt tatctgccat caatgccagt tcgctgaccg tgaaagcatt
    14101 tcttgatatg caggatgata atctgccaaa gctggtggtt tgccagtctt tatccgttat
    14161 gcagggcgta acctacgagc agtttgcatg gttcgtgcgt cagagcgaag agcagatttc
    14221 gatggtcatt cttgaagcta atgcccatca actgctgtta ccgactgacg atgaagggca
    14281 aaacaacgtt accgaaaact atttcctcca ctgataactc ctttcgagca cgcagtcgct
    14341 ggtgcagtga ctgcgcactg caaaattatc tgctgttttt aaccttttct taaagattat
    14401 ttcacttctc ttgtgccgat ttggctttat cacatagagc aaatacgcat aacaatttgt
    14461 taaataccgt tttttaatcc gagctatagt ctcaaaccct ggctaaagtt attcttgcga
    14521 tgcttttata tagcgagcag tgctggccgg gagagagtta tcttttctta caccgcgccg
    14581 ataaaaaata tgcacgttta ttgcatatct tttagtatga caacttttgt tcgtttgtta
    14641 acgaactttc agaaggaaag agatatgacc gccttaaata aaaaatggct ttcgggtctg
    14701 gttgcgggtg ctctgatggc cgtctctgtc ggcacgctcg cggctgaaca aaaaacactc
    14761 cacatttata actggtctga ttatatcgcc cctgacacgg tggccaattt tgaaaaagaa
    14821 accggtatta aagtcgtcta cgatgttttc gactctaacg aagtactgga aggcaaatta
    14881 atggcgggta gcaccggctt tgacttggtg gttccatctg ccagctttct tgaacgccag
    14941 ttgactgcgg gagttttcca gccgctggac aaaagcaaat tgccggagtg gaagaatctc
    15001 gacccggagc tgctgaagct ggtcgccaaa cacgatcccg acaataaatt tgctatgccc
    15061 tatatgtggg cgacgaccgg gattggctat aacgttgata aagttaaagc tgtgctgggc
    15121 gaaaacgcgc ccgtcgatag ctgggacttg atcctcaaac ctgaaaatct ggaaaaactg
    15181 aaaagctgcg gtgtctcttt cctggatgcg ccagaagaag tttttgctac cgtgttgaat
    15241 tatctcggca aagatcccaa cagcactaaa gcggatgatt acaccggacc ggcaaccgat
    15301 ctgctgttaa agctgcgccc gaacattcgt tatttccatt catctcaata cattaacgac
    15361 ctggcaaacg gcgatatttg cgtcgctatc ggctgggcag gtgatgtctg gcaggcgtca
    15421 aaccgcgcga aggaagcgaa gaatggagtg aatgtctcgt tctcgattcc aaaagaaggg
    15481 gcgatggcgt tctttgatgt attcgccatg cctgcggatg ccaaaaacaa agacgaagcc
    15541 tatcagttcc tgaattacct gctgcgcccg gatgtagtag cgcatatttc cgaccatgtg
    15601 ttctatgcca acgccaataa agcagccacg ccgctggtga gtgcggaagt ccgtgagaac
    15661 ccgggtattt atcctccggc ggacgttcgt gcgaagctat tcactctgaa agtacaggat
    15721 ccgaaaatcg accgtgtgcg cacccgcgcg tggaccaaag tgaagagcgg aaaataatcc
    15781 gcagccttag atgccgggcg ggcgcaccac acccgccggc aattcgcacc atcatggtgc
    15841 gcttgcacac attcaatgcc ggagagcagc cgtgaatgac gctatccctc gcccgcaggc
    15901 gaaaacccgt aaggcgctga cgccgctatt agaaatccgc aacctcacca aatcctacga
    15961 tggtcagcat gcggtcgatg atgtcagcct gaccatctac aaaggtgaaa tcttcgcgct
    16021 gctgggcgct tccggctgtg gcaagtccac gcagctgcgt atgctggcag gttttgaaca
    16081 accttctgcc gggcagataa tgctcgatgg cgtcgatttg tcacaggtgc cgccttacct
    16141 gcgccccatc aatatgatgt ttcagtctta cgcgctgttt ccgcatatga ccgtggaaca
    16201 gaacatcgct tttggcctga aacaggacaa actaccgaaa gcggaaattg ccagtcgggt
    16261 ccatgagatg ctcggcctgg tgcacatgca ggagttcgcc aaacgcaaac cgcatcagct
    16321 ttccggtggt cagcgacagc gtgtggcgct ggcccgaagc cttgccaagc gcccgaaact
    16381 attactgctc gacgagccga tgggcgcgct ggataaaaag ctgcgtgacc ggatgcagct
    16441 tgaagtggtg gatattctgg agcgtgtcgg cgtgacctgt gtgatggtca ctcacgatca
    16501 ggaagaggcg atgacgatgg cgggacgtat cgccattatg aatcgcggga aatttgtcca
    16561 gattggcgag ccagaagaga tctacgaaca tccaaccacc cgctacagtg ctgaatttat
    16621 cggttcggtt aatgtctttg aaggtgtact caaagagcgt catgaggatg ggcttgtgct
    16681 ttattcgcct gggctggtgc atccactgaa agtcgacgcg gatgcctcgg tggtcgataa
    16741 cgtgccggta catgtggcgc tgcgcccgga aaaaatcatg ctttgcgaag agccgcccgc
    16801 caatggttgt aacttcgcgg tgggggaggt gatacacatt gcctatctcg gcgatctttc
    16861 ggtgtatcac gttcgtctga aaagtgggca gatgattagc gcccagctac aaaacgccca
    16921 tcgtcatcgt aaagggttac cgacctgggg cgacgaagtg cgtttgtgct gggaagtgga
    16981 cagctgtgtg gtgctgacgg tttaaggagc aaagatgaat acacttgaac ctgctgccca
    17041 gtcgaaaccg ccgggcggat ttaagctgtg gttgtcgcag ctgcaaatga agcatgggcg
    17101 caaactggtc attgcgttgc catatatctg gttgatcttg ctgtttctgc tgccatttct
    17161 gattgtcttt aaaataagcc tggcggagat ggcgcgcgct attccacctt ataccgaact
    17221 gatggagtgg gctgacgggc aactgtccat cactcttaat ctcggtaatt ttctgcaact
    17281 gaccgacgat ccgctctatt tcgatgctta tctccagtcg ctacaggtgg ctgcgatttc
    17341 gacattttgc tgtttactga tcggctatcc gctggcgtgg gcggtggcgc acagtaagcc
    17401 ttcgacccgt aatattttat tactactggt gatcctgccg tcgtggacct cgtttctgat
    17461 ccgcgtttat gcctggatgg gaatattaaa aaacaacggt gtgctgaata attttctgct
    17521 gtggctgggg gttatcgatc aaccgctgac cattcttcat accaatctgg cagtttatat
    17581 tggcattgtt tacgcctacg tgccgtttat ggtgctgccg atttataccg cgttgattcg
    17641 tattgattat tcgctagtgg aagcagcgct ggatctcggt gcacgaccgc tgaaaacgtt
    17701 ctttactgtg atcgtgccgc tgactaaagg tgggattatt gccggatcga tgctggtgtt
    17761 tatcccggct gtgggcgagt ttgtgatccc ggaactgctc ggtggcccgg acagcatcat
    17821 gatcgggcgc gtgctatggc aagagttttt caataaccgc gactggccgg tggccgcggc
    17881 ggtagcgatc atcatgttgc tgctgctaat tgtgccgata atgtggttcc acaaacacca
    17941 gcaaaaaagc gtgggagaac acggatgaat aatttaccgg tagttcgttc gccctggcgg
    18001 attgtgattt tgctgctggg ctttaccttt ctctacgcgc caatgctgat gctggttatc
    18061 tattcgttta acagctcgaa gctggtgacg gtgtgggccg gctggtcaac gcgctggtat
    18121 ggtgagttat tgcgcgatga cgcgatgatg agtgcggttg gtttaagcct gacaattgct
    18181 gcctgtgcgg caacggcggc ggcgatcctc gggactattg cggcggtggt gctggtgcgc
    18241 tttggcaggt ttcgcggatc aaatggcttt gcctttatga tcacagcgcc gctggtaatg
    18301 ccagatgtca tcacgggctt gtcgctgttg ttattattcg tcgcgcttgc tcatgccatt
    18361 ggctggcctg cggaccgcgg tatgcttacc atctggctgg cgcatgtcac tttctgtacg
    18421 gcttatgtgg cggtcgttat ttcgtcgcgt ctgcgggaac tggatcgctc gatagaagaa
    18481 gcagcgatgg atctcggtgc gacgccgctg aaagtgtttt tcgtcattac gctaccgatg
    18541 atcatgcctg cgatcatttc tggctggtta ctggctttta ctttgtcgct tgatgatctg
    18601 gtgatcgcca gctttgtttc cgggccggga gccaccacgt taccgatgct ggtcttttcc
    18661 agcgtgcgga tgggggtgaa tccggaaatc aacgcccttg caacgttaat tctcggtgcg
    18721 gtcggaattg tcggatttat cgcctggtat ctgatggctc gcgcagaaaa acagcggata
    18781 cgcgatatcc agcgtgcaag acatggctga agacactaaa atttgccaac ctggctacat
    18841 aatgccgcgc atgccgcggc attgttttca tggaagacga aacgttggga ttttttaaga
    18901 aaacatcttc atctcatgct cgcctgaatg tgcctgcgct ggtgcaggta gcggcgctcg
    18961 ccattattat gatccgtggc ctcgacgtgc tgatgatttt caatacgctg ggcgtgcgcg
    19021 gtattggcga gttcattcat cgcagcgtac aaacctggag cttaacgctg gtatttttaa
    19081 gcagtctggt gctggttttt atcgagatct ggtgtgcgtt ttcactggtg aaagggcgtc
    19141 gctgggcgcg ctggctatat ctgctgacac aaatcaccgc cgcaagttac ttgtgggcgg
    19201 cttcgctggg gtatggttat ccggagctgt tcagcattcc cggtgaatca aaacgtgaaa
    19261 tcttccatag cctgatgctg cagaagctgc cggatatgct catcctgatg ctgctgttcg
    19321 ttccctcgac cagtcggcgg ttcttccagt tgcaataatg tgtataatcg tcgcccctga
    19381 tgatgtgaag gtcaatgtat gcagtgcgca ctttacgacg cgggtcgctg tcgttcctgt
    19441 cagtggataa cgcagccgat tccagagcaa ctctccgcta aaaccgccga tcttaaaaat
    19501 ctgctcgccg attttccggt tgaggaatgg tgcgcgccgg tgtcaggccc ggaacaaggg
    19561 tttcgtaata aagccaaaat ggtggtgagt ggtagcgttg aaaaaccact gctcggtatg
    19621 ctgcatcgcg atggcacgcc ggaagacctt tgtgactgcc cgctttatcc tgcctctttt
    19681 gcgcccgttt ttgcggcgct aaaaccgttt atcgcccgtg cggggttaac gccctacaac
    19741 gtggcgcgta agcgtggcga actgaaatac attctgctga ctgaaagcca gagcgatggc
    19801 ggcatgatgc tgcgctttgt actgcgttct gaaaccaaac tggcacaact gcgtaaggcg
    19861 ctgccgtggt tacaggaaca actaccgcaa ctgaaagtta ttaccgtcaa tattcagccg
    19921 gtacatatgg cgattatgga aggggagacg gagatctacc tgactgaaca acaggcgctg
    19981 gcagagcgtt ttaatgacgt accgctgtgg atccgtccgc aaagtttctt ccagactaat
    20041 ccggcggtcg ccagccagct gtacgctacc gcgcgcgact gggtacggca gctgccagtt
    20101 aaacatatgt gggatctgtt ctgcggtgtg gggggctttg gtttacactg cgcgacgcct
    20161 gacatgcagt taaccgggat cgaaattgcg ccagaggcca ttgcctgtgc aaagcagtca
    20221 gccgctgaac tgggcttaac tcgtttacaa tttcaggcgc tggactccac tcagtttgcc
    20281 actgctcagg gggaggtgcc ggagctggtg ctggttaacc cgccgcgccg cggcattggt
    20341 aaaccgctgt gtgattatct ctcaacgatg gcaccgcgtt ttatcatcta ctccagctgt
    20401 aacgcccaaa ctatggcgaa agatatccgc gaactgcctg ggtttcgcat cgaacgggta
    20461 cagctttttg atatgttccc gcacaccgcg cactatgaag tgctgacgct gctggtgaag
    20521 caataaaaaa gccgcaggtg cggcttcaga ttgctgatca ggtgcgcttt gttcatgccg
    20581 gatgcggcgt gtacgcctta tccggcctac aaaatcatgc taaatcaata cattgcagag
    20641 ttgatgtggg ccagataagc gcagcgcatc aggcaatctt acttttgtca tcagtctcaa
    20701 ttactgcggg aaccactggt cattgatttt ctgatatgta ccgtcagctt taattgctgc
    20761 cagcgcgtta ttcagttttt ccagcagagc tttgttatcc ggacgtacag cgatgcccag
    20821 accggtgccg aagtattgcg gatcggtcac tttttcagta gcaacgccca gttgcggatt
    20881 ggttttcagc cattcgttta ccaccgctgt gtcaccaaat accccatcaa tacgaccatt
    20941 tttcagatcg ataaaggcat tctgataact gtcataggag acagttttca cttcggggtg
    21001 ctgatcctga atatatttct ggtgcgtggt gccgttttcc atcccaatac gtttgccttt
    21061 cagatcggca aacgttttgt aggtatcttt tttggcaatc acgacggctg agttttcata
    21121 gtagggcgtg gtaaacgata cctgcttgct acgctcgggc gtgatatcca taccggagat
    21181 tacggcgtca tattttctga atttcagtga cgggatcagg ctgtcgaacg cgtgattagt
    21241 aaaagtacat tctgcctgca tttgtttgca caaggctttt gccagatcga tatcaaagcc
    21301 gacaatctca ttattagcac ctatagattc aaagggtgga taggtggctg aaacgccaaa
    21361 attgattttc tctgcggcag aagcaccgaa agtaaaggaa gcaagtaaag cggcaagaac
    21421 taactttttc atgatggaac tcccgtctgt caatcttatg atttttggcc gtgtctgcgg
    21481 catgggataa caatgccatc aagtgaattt atatgcaata aatatgatta aataatttaa
    21541 atgaaataaa aaacgacgga taacttagtg agttgtccgt cttcattata agaatttatg
    21601 cactatgtag gccggataag gcgtccacgc cgcatccggc acaggcaccg tgctgatgtc
    21661 tgatgcgacg ctggcgcgtc ttatcagacc tacaaacccc ccggcgaatg tacgcagcta
    21721 cattaattcc gccgttcgaa tgccagtgct ttgcgctcga tcagacgcat catcagcgtc
    21781 agcaggccgt tgacgaccag gtaaataatc cctgccgcac cgaacaccat tacatcgtag
    21841 gtgcgtccgt acaacaactg gctgtatccc atcacttcca tcagcgtaat ggtgtaagcc
    21901 agagaggtac ttttgaacac cagcaccact tcgttggaat aagaagagag cgagcgttta
    21961 aaggcatacg gcagcaggat cgccagcgta tcttttttgc tcattcccag ggcgctacag
    22021 gactgccact gaccttctgg gatcgcacga attgcaccgt aaaacagctg cgtggtatac
    22081 gccgcactgt tcagcgataa cgcaatcagc gcacataacc acggttctga caacaaatgc
    22141 cacagtgccg gatactcctg caaagtcgga aactggcccg gcccgtaata aatcaggaag
    22201 atctgcacca gcagcggcgt accggtaaac agcgtgatat aaccccgcac cagccacacc
    22261 agcaccggcg ttttcagcgt caggatgatg gtaaaaatca atgccagaat cagtgccaca
    22321 atcagcgagg caacggttag cgtcaggctg gtgtgtagcc ctttcatcag ttcgggtaaa
    22381 tactcaaaca ttagctgggc ctccgctcaa aacgtgtcgc gcgcaggtca atgcgtttga
    22441 gaatgtactg actgagcagg gtgatcacca ggtaaatcgc cgccgccaca atataccagg
    22501 taaatggttc ctgggtacga gtagcgatgc tttttgtttg cagcattaaa tcattcacac
    22561 taatcaaact gaccagcgcg gtatctttca gcagcaccag ccactggtta ccgaggccag
    22621 gcagcgcatg acgccacatc tgcggcatca ccagacggaa aaagatagcc gatttcgaca
    22681 gccccagcgc ctgaccagac tcccactgac ccaccggcac cgctttcaac gcgccccgaa
    22741 gcgtttgcga ggcataggcg gcatacagca gtgacagagc gatgacacca caaaggaacg
    22801 ggctaacgtc gaagttctca atgtccatct gcactgggat ctgcacgaac ccaagattga
    22861 tagtgaagcc atccgaaagc gtcagcagca gctgcgagga gccaaaatag ataaacagca
    22921 ccaccagaat ttctggcagg ccacgcagaa tggttaccag cgctgaacct gcccacgcga
    22981 caggacgcca tttttccgac tcccataccg caaagaacat cgccagcgcc agcccgacaa
    23041 tcaatgcaca aacggcaagg ccgacggtca tcccggcggc gcttgctaaa ggaaaaaatt
    23101 cattcatcag gaattacttc tggaaccatt tgttgtagat ggtttcgtaa gtgccatctt
    23161 tcttcacttt ttccagcgca gtgttgagtt tctgctgcag ctcagtgttg ccctgacgta
    23221 ccgcgatgcc gaggccagtg ccgaagtaat ctttatcggt cactttgtcg cccaccgccg
    23281 ccagtttcgg gttatctttc agccactcag tgaccactgc ggtgtcaccg aagacgccgt
    23341 cgatacgccc gttttgcaga tccagttttg cgttctggta gctgtcatac ggaacggtag
    23401 tgatttccgg gtgcttatcc ataatgaatt tctggtgtgt cgtcccgttc tgtacgccga
    23461 cttttttgcc tttcagctga tcaacactgg tgtatttgcc ttgctgaccc acaaacaggg
    23521 cagagttgtc atagtacggg gtggtaaaca gcacctgctt ttcacgctcc ggagtgatat
    23581 ccatgcccgc catcacggct tctacgcgac ggaatttcag gcttgggatc aggctgtcaa
    23641 acgcctggtt agagaaagtg caggttgcat caatctcttt acacagcgct tgtgccaggt
    23701 cgacgtcaaa accaacgatc tggttgtttg catcaatcga ttcaaacgga ggataggatg
    23761 cttcggtagc aaaacgaatg gtttcggcag ctgtggcgga aagactaaaa cctgcaatta
    23821 acgcggcaat cagaactttt ttcattgttg ttatcccgaa tcttagtgag agagatagtt
    23881 tttaaatgct tcggtttgcg gctcggtaaa gcagctcgcg tcgccttgtt ctacgatatg
    23941 accattttcc atatacacca ctcggctggc ggttttacgc gccacttcaa cttcgtgggt
    24001 gacgatcacc tgggtaatat tcgtttctgc cagctcacga atgatgctga cgatttgtgc
    24061 cgtaatttcc gggtccagtg cggcggtcgg ttcatcgaac agcagtacct gcggttccat
    24121 catcaacgca cgggcaatag caacacgctg ctgctgacca ccagaaagat gcagcgggta
    24181 acgatcgcta taaggtttga gacgcagacg ttccagcagt ttttctgcac gggccagcgc
    24241 ctgatcttta ctcaacccca gtacacggca gggcgcttca atcaggtttt gctgcacggt
    24301 cagatgcggc cacaggttgt attgctgaaa caccatgcca acgttacgac gcaaatcgcg
    24361 aatcgctttg tcagaggggg ttttggtgaa atcgaaatgg ttgcctgcaa tgttgagcgt
    24421 accggagcgc ggcatctcaa gcagattgag tacacgcagc agcgagcttt tacccgcgcc
    24481 gctggggcca agtaacacca gcgtttcgcc ctgtgggcaa tccagcgtga tatcgaacag
    24541 cgcctgatgc gcgccgtaga agcaattaat gccgtttaat tgaatactca ttgacactcg
    24601 tatactggca gtctgatagc tattgaggtc gaagatagta cctttgacag aataattatg
    24661 caatatttct gctttaaaag ttaaaagcaa agcgcattat tcaataaaca tagcacaaaa
    24721 taacgggggc ggtggtcggc gagcataaat gtcggcattc ctcacgaaat gccggacaat
    24781 ttacggggtt tattggttga tcaaggcgtt agcgattctc gatggactga cggagcgtac
    24841 ccgccgtggc atgaacgctg ccgcctaagt agcgtacatc gtcgatcacc caacactggc
    24901 cttcctgaat cattaacact tcatcctgcc agctctgatc accctgtttt agatccacac
    24961 gcagaggaat atttcgcgca tcacgattcg ggattgttga ggcactggca acacgggcgc
    25021 tctctggcaa ggttgtgcga ctggagaatg gatcgttggt cagtagttcc cgatggttat
    25081 tatcccggga ggcatcgcta agcagtgtcg ccagtttgtc gctcagataa gggcgcaagg
    25141 cggtgatgtc gttgctgcgg tgcaaaatgc ggtagtcata aaattgctgg gccacgttac
    25201 ccgggcctcc ttcaacgcaa ggaccactgc gtgtgccgtt atctttataa gctggagtga
    25261 ctgtggtgca ggcactgagg agcagtgcgc aggggataag cattgtcaat ttgctgtagc
    25321 gcataatgat ttccttataa gcgatcgctc tgaaagcgtt ctacgataat aatgatatcc
    25381 tttcaataat agcgtatcag tctgataatg cttttgagat cgaaggctta gcaaacaagg
    25441 agatcgatca tgcaattttc tacaacccca actctggaag gccagaccat cgttgaatat
    25501 tgcggtgtgg tgaccggcga agcgatttta ggtgccaata ttttccgtga tttctttgcc
    25561 ggtatccgcg atatcgttgg cggacgttcc ggtgcgtatg aaaaagaact gcgtaaagca
    25621 cgggagatcg cctttgagga attaggctcc caggcgcggg cgctgggggc cgatgccgtc
    25681 gtcggtattg atatcgacta cgaaacggtc gggcaaaacg gcagtatgct gatggttagc
    25741 gtcagcggta cggcggtgaa aacgcgtcga tgagaagagt cttctggctg gtcgctgccg
    25801 ctctgttatt ggcagggtgt gcaggcgaaa aaggcattgt cgagaaagag ggatatcagc
    25861 ttgatacccg acgccaggcg caggcggcgt atccgcgcat taaagtgctg gtgatccact
    25921 acaccgcaga tgattttgat agctcgctgg cgacactgac cgataagcag gtcagctcgc
    25981 attatctggt ccctgcggta ccaccgcgat acaacggtaa accgcgcatc tggcaactgg
    26041 tgccggaaca agaactggcc tggcatgcgg ggattagcgc ctggcgcggg gcaacgcgcc
    26101 ttaacgacac ctctattggc attgagctgg aaaaccgtgg ctggcaaaaa tcggccggag
    26161 tgaaatattt tgccccgttt gaaccggcac agattcaggc gcttattcca ctggcgaaag
    26221 atattattgc ccgttatcac atcaagccgg aaaacgtagt ggcacatgcg gatatcgcac
    26281 cgcagcgcaa agacgatccg gggccattat ttccctggca gcaactggcg cagcagggga
    26341 ttggtgcctg gccggatgcg cagcgggtta acttttacct tgccgggcgc gcgccgcaca
    26401 caccggtaga taccgcgtca ttgctggagc ttttggcgcg ctacggttat gacgttaaac
    26461 ctgatatgac accgcgcgag cagcggcgcg tgattatggc attccagatg catttccgcc
    26521 cgacgttata taacggcgaa gcggatgcag aaactcaggc gattgccgaa gcattgctgg
    26581 agaaatacgg gcaggattag cgcggcagtt ttccgtggtc gcgtagccag gcggcagttt
    26641 tctcgatacc ttcatccagg gtgatgaccg gctgataacc taactcttcc tgcgcacgcg
    26701 taatatccag cgtaaagtca aaattcaact tggagacgcc gtagtgggtc agcggcggct
    26761 cttttgctga cttgcggaaa cgctccatgc tgcgggcgat catatccagc atcgggtagg
    26821 ggacggaacg aatacgacag tcaatattca actcgtcgat cagcttctgc acgatgctgc
    26881 acagtgtgcg atgctcgccg ttggtgatgt tgtacacacg cccggaaggt agcttatcgc
    26941 aggcttcctg gcttgccagc cacattgcgt gcacggcatt ttcatagtag gtcatatcga
    27001 ccagcgcact gccgccatgc ggtaacagaa tactgccgta gtggtgcatc atatgcgcca
    27061 gacggggaat aaagacttta tcgtgcggtc cgaacagact ttgtgggcgc agaatagtaa
    27121 agcgcgtttg tggattcgcc tgcgaaaaca tattgatcac ttcttcgctg gctgctttgc
    27181 tgcgggcaaa ctcgttggcg aagcggtgag ggcgaaaatc ttctttaata tcgcgatggt
    27241 ggtgataatc gaagtacagg gagggggaag agatatgaat aaagttacgc acaccccagg
    27301 cgacagccca ttcacccagg cggcgagtgg cgcgaacgtt agccagatcg aaagcctgtt
    27361 gtgtccccca gggtgaggta aagctggagc agtgccacag cgtatcaatg cccgcgagca
    27421 tcactttagc ttgtgatgaa accagctcgg tcagatccgc cggaacaaac tctgcgccca
    27481 ttttttccag caatttgccc attgcctcgt tgcgaccggt cgctcgcacg ctgatgcctt
    27541 tctggcataa aaactctacc gcgtttcgac ctaagccgct ggtggcgccg gtaaccagta
    27601 ccttcatatc aatccactgt tgttgagaaa ataacgtgcg cattcttccg tgatttcccc
    27661 catgatgcaa tgggaaacat gaaagaataa cgcaggtttt gtcgattaat ctgtgctttg
    27721 ttctgccagt ctggcgattt gttttgccat tccgcgaaaa ataaacagat gcgcggggat
    27781 catcaataac cagtaaaaca gccccggcat accgtgcgga tgccagaaag cgcggacatc
    27841 gatagtacga tagtcgcctt tatcttccag gctaaaacac agtcgtccca gccccggcgc
    27901 tttcatgcca aataacaacg taagttgttt ttccggttca acgacaatca ctttccagct
    27961 atctaccgca tcgccagtct gtaaatattc gcgctccggg cggcctttcg ccagcttatg
    28021 accgatcgcg cggtccatca acgcccgtgt ctgccacaaa atattgccaa agaaataacg
    28081 ctctttaccg ccgatttggt tcactacctg ccataaagca gcaaggctgg cggacgtttt
    28141 aacggtaaac cccgcctgtt tggcaaaata accgtactcc ggtcgccagc gggcaaaggc
    28201 ctgagcgtcg tagccccagt cgctggagtt gaccagtttt tcctcctctt tcaacgtgct
    28261 acgtactgcg tcatcgaaag cgatcagccg ttgtgggatg agtgcacgta gcgcggtatc
    28321 atccgccagc agatcgtgtt tcagcccctg aatcaacgcc ctggcggtgg tgggcggtac
    28381 ggaagtaatc acattgagaa accacaccga aatccagcgg gtggggaggg ggatggggat
    28441 caaccagcgg cgcttgccgc tcaccgccat aaaatgttca aactgttgct gataactgag
    28501 cacctctggt ccggcggctt cgaagatgcg gtgttcgctg gccggatgat ctaacaacgc
    28561 caccagatag tgcagcaagt tttccagcgc gatgggcgtg gtgcgtgaac gtacccagcg
    28621 tggcggcgtt aacactggca ggttgtagac catatcgcgc atgacttcga acgccgctga
    28681 acctgcgcca acgataattc cggctcgaag ttcggtcaca ggtacattcg cttcacgaag
    28741 aatgtccgcc gtagcctgac gagcacgcag atgatccgac tgctcatgtg gcggggcctg
    28801 caacgaactg agaaagatta attgcttaac tggtacttca cgtagcgcat cgcggacgtt
    28861 gagagccacc tggcgctcct gagcgataaa atcgccgcct tcgcccatgc tgtgcaccag
    28921 aaaatagacg gtatcgatat cctgcaacag ggccggaagg ttatccggcc agctgagatc
    28981 gactttatgg caactgacgt ttgccagttg cagctttgca agcctgtcga catgacgtgc
    29041 cgccgccagg atctgatgcc cttgctggct gagtgtgcgc accagatgct gaccaatgta
    29101 gccactggca ccgagaacta aaatgcgttg cggcacgtct ctctccttaa cgcgccagga
    29161 atgcgcgcca gtgggcggcg acttccgcca gttgttcgcg cgagacgtca agatgcgtca
    29221 ccaggcggac aatcggcgag gcgttaatca gcacgtttct cactttcatg tattcgccta
    29281 acgcggcagc attttcttcc ccgacgcgaa caaacagcat attagtgtcc tgacgcatca
    29341 catccgcgcc tgcttcacgc agttgctccg ccatccaggc ggcgttgtcg tgatcttcct
    29401 gcaaccgtgc gacgttattt ttcagggcat acatcccggc ggcagccaga atcccggact
    29461 gacgcatccc gccaccggcc attttccgcc agcggatggc acgtttaatg taatcacgat
    29521 taccgacgag taatgaaccg acaggcgtcc caagaccttt cgacaggcaa atggtgaacg
    29581 aatcacaata ttgcgtgatc tctttcagtt cgcagccgta agccaccacg gcattaaaga
    29641 tgcgcgcacc gtcaacatgc agcgccagat tgcgctcgcg ggtaaattcc catgcttctt
    29701 tcaggtattc acgcggcagc actttgccgt tgtgggtgtt ttccagactg agtaatttgg
    29761 tgcgggcgaa atggatatcg tcgggtttga ttttcatcgc cactttatcc agcggtagcg
    29821 tgccgtcggc agccgcgtcg atgggttgcg gctgaatact gcccagcacc gctgcgccac
    29881 cggcttcaaa cagatagtta tgcgcggcct gaccgacaat atactcttcg ccacgttcgc
    29941 agtgactgag cagagcgacc aggttggcct gagtgccggt aggcagaaaa atggcggctt
    30001 ctttaccgga aagctctgct gcatagtcct gcagagcatt aacggtaggg tcgtctccgt
    30061 aaacgtcgtc cccaaccggg gcggccatca tcgcttcgag catggcgcgg ctcggtcggg
    30121 taacggtatc actgcgtaaa tcaatcatgg catgtcctta ttatgacggg aaatgccacc
    30181 ctttttacct tagccagttc gttttcgcca gttcgatcac ttcatcgccg cgtccgctga
    30241 tgattgcgcg caacatatac aagctaaatc ctttggcctg ttcgagtttg atctgcggtg
    30301 gaatggctaa ctcttctttg gcgaccacca catccaccag caccggaccg tcgatggaga
    30361 aggcgcgttg cagggcttca tcaacttcag acgctttttc tacacggata cccgtaatgc
    30421 cgcacgcttc ggcaatgcgg gcaaagtttg tgtcgtgtag ttcggtgccg tcagtcaaat
    30481 agccaccagc tttcatctcc atcgccacaa agcccagcac gctgttgtta aagacgacaa
    30541 ttttcactgg cagtttcatc tgcactactg agaggaaatc gcccatcaac atgctaaaac
    30601 cgccatcgcc gcacatggcg accacctgac gttctggctc tgtcgcctgc gcacccagcg
    30661 cctgcggcat ggcgttagcc atcgaaccgt ggttaaacga acctaacagg cgacgcttgc
    30721 cgttcatttt tagataacgt gccgcccaca ccgttggcgt accaacgtca caggtgaaaa
    30781 tagcgtcatc ggcggcaaaa tgactaattt gctgcgccag atattgcggg tgaatggctt
    30841 tctcgctcgg tttagctaaa tcgtccagcc ctttgcgggc gtcgcggtaa tcttccagcg
    30901 ctttatccag aaacttgcga tcggcttttt cttccaccaa tggaagcaat gcacgcagag
    30961 tcgacttgat atcgccgacc agtgccatat ccaccttgct gtgagcgccg atgctggctg
    31021 ggttgatatc aatctgaatg attttggcat cggtcgggta gaaggcgcgg tagggaaatt
    31081 gcgtgccgag tagcactaac gtgtcggcgt tcatcatggt atggaaacct gacgagaagc
    31141 cgattaaccc ggtcattcca acatcatacg gattatcgta ttcgacatgt tctttaccgc
    31201 gcagggcatg aacaataggc gctttaattt tcccggcaaa ctcaactaac tctttatgcg
    31261 cccccgcgca gccgctgcca cacatcaggg cgatattgct ggaataacgc agcagttgcg
    31321 ccagtttgcg taactcttct tcttccggcg tcacgactgg ttgtggcgca tgataccagt
    31381 gcgtggttgc cccttctggc gcaggtttta acgccacgtc gcctggtaac acgacaaccg
    31441 aaacgccacg gttaagcacc gctttgcgca tggcaatcgc cagtacttgt gggatctgct
    31501 ccgggctgga aaccagctcg caatagtgac tacattcgcg gaatagctct tgtgggtggg
    31561 tttcctggaa atagccgctg ccaatttcgc tggagggaat atgagcggca atcgccagta
    31621 ccggaacgtg attgcggtgg caatcaaaca ggccgttgat taagtgcagg ttgccggggc
    31681 cgcacgatcc ggcgcagacc gccagttctc cgctaagttg tgcttcagcg ccagcggcaa
    31741 aggccgccac ttcttcgtgg cgggtggaca tccactcgat ggtgcccatg cgattaagac
    31801 tgtcactaag accattcaga gagtcgcctg tgactcccca gatgcgtttc acccctgccg
    31861 attcgagtgt tttggcgata taagctgcaa ccgtttgttt catggttctc catctcctga
    31921 atgtgataac ggtaacaagt ttagttcatc tgacggaggg ggaagggatg ggagagaaag
    31981 gaggcactaa cggttaaata gcccgatgaa aggaatatca tcgggcataa ggcgattatg
    32041 cgagaaccaa atccccctgc ggatggcagg agcaggccag tacgtaacct tcagcgattt
    32101 cggcgtcggt cagcgtcatt gtactgctca ccgtatattc accggaaacc acttttgtct
    32161 tacagcagcc gcaaacaccc gcacggcagg cagcgacaac cggaacgtta ttgctttcca
    32221 gcgcctccag tagtgtggtg ccaaccgggg cgtaaaattc tcgtgccggt tgcagtttgg
    32281 tgaatttcag accgctggtc gccgcttttg ctactggggt gaagaatttc tctttaaaga
    32341 aacgcgtcac gccgagcgct ttcacttcct gctctaccca atccatatac ggagccgggc
    32401 cgcaggtcat cacggtacgt gaagctaagt caggtacacc tgccagcagt tctcgagtga
    32461 gacgaccagc gataaagcct tcggtaacgt tattttctgc caccagcgtt accggatagt
    32521 tacgccactc atcggcgaaa ataacatcct gcggcgtatg cacgttgtag atcacccgca
    32581 catcggcctg tggacggttc ttcgcaagcc agcgacgcat cgacataatc ggcgtgacgc
    32641 cgcagcctgc cgccagcaac aggaatttat cttctgcttt atcgtcgcag gtaaattccc
    32701 ccatcgcgtc cgaaagccag agataatcac cgcgttttac atcgcgcgtc agccactggg
    32761 agccgacacc gtcatcaatc cgccgcacgg tcagggtgat atattcactc acgcctggcg
    32821 tggaggaaat ggtgtaagca cgcagcgttt ccgctgagtt tcgcacgctg accagtgcat
    32881 attgcccggc gcgatatggg tagtaatcgt ggcaaatcag ggaaatcgtc cacacatccg
    32941 gcgtttcttg cgtaatgtga tgaacctgca tccgccacgg gcattgattc gttggcatcg
    33001 tcatcgacaa actccttacg cgctcaacag ttgcttcatg tcttcttcaa cagtggtgat
    33061 agaacgcagg ccgaatttct cgttcagcac cgccagcagg tctggtgtca ggaaaccagg
    33121 tgcagtcggg ccggtgacga tatttttcac gcccagagaa agcagcgtca gcagaatgac
    33181 gatcgctttc tgttcaaacc aggagagcac cagcgacagc ggcagatcgt tcacaccaca
    33241 gcccagtttc tctgccagag tgacagccag aataatcgct gagtaagcat cgttacattg
    33301 acctgcatct accagacgcg gcagaccttc gatatcgcca aactcaagtt tgttaaagcg
    33361 atatttacca caggcgaggg tcaggatcag gcagtcatcc ggcacgatgg tggcgaaatc
    33421 ggtgaagtag tggcgctcgc cgcgtgcgcc gtcacagcca ccaagcagga agatatgacg
    33481 cagtttttca cggctcacca gatcaatcag cgtatcagca gcgccaagca gcgtctggcg
    33541 accaaaaccc acggtgataa ggtgcggaat ttcgctgtac gggaagcctg ccatctgttg
    33601 cgcctgggtg ataaccgcag agaaatcatc accgtccaga tgacgcacgc taggccagcc
    33661 aacaatgctg cgggtccaga tacgatcgtc ataagcgctt acggttgggt cgatgatgca
    33721 gttcgaggtc atcacgatgg ggccagggaa acgagcgaac tccacttgct gattctgcca
    33781 gccgctgccg tagttaccga ccagatgctt gaatttacgc agctccgggt agccatgcgc
    33841 aggcagcatt tcgccgtggg tgtagacatt aacgcccgtg ccttcggtct gttccagcag
    33901 gttgtagaga tctttgagat cgtgaccgga aatcagaatg catttacccg ccgtcgcttt
    33961 gacgttgacc tgggttggcg tcgggtgacc gtatttaccg gtttcgcctg catccagaat
    34021 gctcatcact ttgaagttca tctggccgat ttccattgaa cactcaagaa gcgcgttcat
    34081 atcggcaggc caggtcccca gccacgccat gattttatgg tactgggcat aaatatcgtt
    34141 gtcgtattga ccgagaacat gcgcgtgttc catataggcc gccgcacctt tcaggccata
    34201 caggcacagc agacgcaggc cgagaatgtt ttcgccaatc gccgctttat ctttgttagg
    34261 ggtaaattct gctgcctgac gttgcagctc gccgagatca tcgctcacca gttgcaggtc
    34321 agccatcggg ttatcgacgc gcgcgttggc atctacagcc aggcattgtg ctttcagcgc
    34381 ctcgcgcaag gcaatcgctt cacgagcgta gccgacaata cgcggagaat cgaagttaac
    34441 gttggtcagg gttgagaaaa aggcacgtgg cgcgaagctg tcaacatcgt ggttgatgat
    34501 gccgtattca cgcgctttta ccgcccaggc agaaagccct tgcagcgccg cgatgagtaa
    34561 atcctgaagg tcagaagttt ccgccgtttt accacacatc ccctgcgcgt atgagcagcc
    34621 gtttcctgcc ggagtacgga tagtttgttc acattgcaca caaaacatga tcacaccttt
    34681 taaagttata tttaatatac atgtttaagg ttaagacgct taacgcgggg ataaaaggga
    34741 tttttcatgc aactttaagg gagattgatt tagcgcaatt ttggcggcag ggatctaccg
    34801 ccagagaggt attacgcaga gaaaaaggcg atgaggatcg gcactaacag gctaagaata
    34861 aaaccgtgaa caattgccgc cgggaccata tccagcccgc cagtacgttg aagaacgggc
    34921 agggtgaaat ccattgatgt ggcaccgcat aagcccagtg cagtagagcg gctgcggcga
    34981 atcagcccag ggatcaacat aatagcaatc agttcacggg ccagatcatt aaaaaacgcc
    35041 gcgctcccga ttaccggacc aaaagattcg gtcaataaaa taccggaaag agaataccag
    35101 ccgaaaccgg aggccattgc cagcgcggta ttgatgggga gatcaagaat aaaggcgtta
    35161 attaaaccac caattaatga actgacaacc accaccacgg cgacaatcat tccccggcga
    35221 ttaaggacaa tctgctttaa ggtcatgcca ttattgcgca actgaatacc aacgaggaaa
    35281 agtagcaaaa ttaacgtgta ttcactggct tcggtcgcgt gttgtaagaa agccagtcca
    35341 ctcagaccaa tggcaaaacc aatcactact acgccgcaca gttttagcga ctccagcgcc
    35401 atcgcaatac gcgacgggag tttttcttgc tgatggtggt tgcgccacgg caggcctcgc
    35461 tccagccaca tcagggcggc aatattacac agtaaaataa cggtaatact gacggcagaa
    35521 taatgcagaa tcgccaacag gttactggcg aggttatcga gaaacgccag actgataccc
    35581 ataaaaaaga gaataaggta aaccatccag cttaatagct gattaataac ttttaacgca
    35641 gcttgttggc gaagcggaat gaggtaaccc acaatcaggg gaaccagaat gattaacagc
    35701 ccagaaaaca tgaaaaccca gtccttgcaa agatgaagtc gaaatgcgcg atgacacact
    35761 actgaaagcg gaaggacgag taaagttgca attaaaagga aatgttatgc ataaggagca
    35821 gtagagtatt cgttttcatt taaagatatt cttgcgcttt aattacaaac tgcaccgatg
    35881 ttggtggcgt caaaatcgcc gaggcgttcc ctgaaggccg gggcagccca catggatgtg
    35941 ggctgagggc gcgttttaca gggatgttac ctcgcgcccg acccggtagc cgtaagggat
    36001 aagtcgaggg caccgcgcag cggcgatttt gttcgccaga gcccgggggg ggcagggggc
    36061 ggcggcgatt ggccgccccc tgcgcgctcc ttgcgccagt ggcaatatgt ggcttagctc
    36121 atgaaaggag cgcaacaaga tgatgaatca acatataaca acatcttaaa aaaagcctga
    36181 cattacgcca ggccttctgc gttaattaat cacgcttttc cagcagggtc cggtaaatca
    36241 gaccaccgat aatgccgccg acaattggca ccacccagaa gaaccacagt tgttctaatg
    36301 cccagccgcc ctggaagata gcaaccgcgg tgctgcgcgc cgggttaaca gaagtgttag
    36361 tcaccggaat actaattaag tgaatcaggg ttaaggccag accaatagcg atcggcgcaa
    36421 aacctgccgg cgcgaatttg gcggttgcgc cgtggatcac caacaggaaa cctgcactca
    36481 ataccagttc aactaccagc gcggaaagca tggaataacc gcctggtgaa tgctcgccat
    36541 aaccgttaga agcaaaaccg ctggctgccg cgtcaaaacc cgttttacca ctggcaatta
    36601 aatacagcag cgccgctgca acaataccgc cgacaacctg ggcaattacg tagccaacga
    36661 cttcttttgc cggaaaacgt ccgccagccc ataaaccaat agtgaccgcc gggttaaaat
    36721 gaccaccaga aatatgacca acagcaaagg ccatcgtcag aacggtcaga ccgaacgcca
    36781 acgccacgcc ggcaaaacca atgcctaatt ccgggaagcc tgcggccagt acagcactac
    36841 cacagccacc aaaaacaagc cagaaagtac caaaacattc agctgctaat tttctgaaca
    36901 tatccacctc aattaaaaat tgaccctgtg aaaaatatgg tcgttttata gggtcgtcgt
    36961 aaaaaaatga cgacggaaat aatgcgcggc tattttaaaa acgaaggcga gtcattcacc
    37021 agataaataa atccagtaaa tttgatttag ggcaacagcg ggttgcccca tatagtcatt
    37081 tgtctgattg acagtgtagt gcacgcaaaa gatttaatcc tttaggcgta ataaaaaata
    37141 atatatcatg ctaattattt gattttgttg tttttgcaga cttatcagca agagggagta
    37201 taacgcgatt attcgctcat ttttcagaca tttgccatgc ttaaatgtga tgtcatcacg
    37261 tattagcaag gcctttcccg ttatactgcc agcgtaaagg ataagtcaca tatttctgga
    37321 ggggatatga ttcttgagcg cgttgaaatt gtgggttttc gcggtatcaa ccgtttgtcg
    37381 ttgatgctgg aacaaaacaa cgtcctgatt ggggagaacg cgtggggtaa atccagcttg
    37441 ctggacgcct taaccctgct gctatcgcca gaatcagatc tctaccattt tgagcgcgac
    37501 gatttctggt tcccgccggg agatatcaac gggcgagaac atcatctgca tattattttg
    37561 accttccgcg aatcgctgcc aggccgacat cgggttcgcc gttatcggcc gctggaagcg
    37621 tgctggacgc catgcaccga tggctatcac cgtatttttt atcgtctgga aggggagagt
    37681 gcggaagacg gcagcgtgat gacactgcgc agttttctcg ataaagacgg acatccgatt
    37741 gatgtcgagg atattaacga tcaggcacgc catctggtgc gtttaatgcc ggtgctgcgc
    37801 ttgcgtgatg cccgttttat gcgccgtatt cgtaacggca cggtgccaaa tgtccctaat
    37861 gtggaagtca ccgcgcgcca gctcgatttc ctcgcccgtg agttatcctc acatccgcaa
    37921 aatctctctg atgggcagat tcgtcaggga ctttccgcaa tggtacagct gcttgagcat
    37981 tatttctctg agcagggggc cggacaggcg cgatatcgtt taatgcggcg gcgggccagc
    38041 aatgagcaac gaagctggcg ctatctggac atcatcaacc ggatgattga ccgacctgga
    38101 gggcgctcgt atcgggttat tttgctcggc ctgtttgcta ctttattgca ggcaaaaggc
    38161 acattgagac tggataaaga cgcgcgtcca ttgttgctga tcgaagatcc agaaacccgt
    38221 ttacacccca ttatgctttc agttgcctgg catctgttga atcttctgcc attgcagcgc
    38281 attgccacca ccaactcggg tgagttgctt tcgttaacgc cggtagagca tgtttgccga
    38341 ctggtacgtg agtcctcgcg cgttgccgcc tggcgtctgg ggccgagtgg cttgagtacc
    38401 gaagatagcc gacgcatatc ctttcacatt cgttttaacc gtccgtcatc gctgtttgca
    38461 cgctgctggt tgctggtgga aggggaaacg gaaacctggg ttatcaatga actggcgcgt
    38521 cagtgcggac atcattttga tgccgaaggg atcaaggtca ttgagtttgc ccagtccggg
    38581 ctaaagccac tggttaaatt tgcccgccga atggggattg aatggcatgt actggtcgat
    38641 ggcgatgaag cagggaagaa atatgccgct acggtacgca gcctgttgaa taacgatcgg
    38701 gaagccgaac gagaacattt aacggcgtta ccggcgctgg atatggaaca ttttatgtat
    38761 cgccagggat tttccgatgt gttccaccgc gtggcgcaaa tcccggaaaa tgtaccgatg
    38821 aatctacgca aaattatctc gaaagcgatc catcgctctt ccaaacccga tcttgccatt
    38881 gaagtggcaa tagaggcagg acgtcgtggt gtggactccg taccgacgct gctgaaaaaa
    38941 atgttctcac gcgtgctgtg gctggcgcgc ggtcgcgcgg attaaccgcg aaacatcgtg
    39001 gccatttgtg gctgaatagc gtcgagcatc tcatagcgcc gacggtattc agcccgtttt
    39061 ttactggcga tttcggcaat ctcttttcgt gctatctgtg ctggaaggcg gtaatggcgt
    39121 tcagcatcac atacgccgcc aaccgattcc cagaaagcgt tgtaatcagc gtggatcttg
    39181 ccttctttat cgcgataacg caggctgcgg taaatatgcg tttcattgct gacggcaata
    39241 atctgctcta cctgcaaacg ttgggcaaac agacaggccg cttccatcac gaggcgtttg
    39301 ggaaatagcc cgtggcaggc tttcgtcgca ttctggattt cctgatgtgg aatttcccat
    39361 tttgcgcctt gcagtccgcc aataaacatc gttcttttcc cctgatattc acacagggta
    39421 aacgtgatct ctgccagagg aataccttcg ctgttgcgaa acaggattgt gctgtcacct
    39481 tctttatcca ttgagatcat catggtcagc ttaagcgtga actgctcgcc gtttttgcct
    39541 tccagcttcg ccagttgcag cccgggggta ttcaaatata agctgaattc ttccgccgac
    39601 atacatccgc ggagtaacgc ataatggtaa cgtaacgcct ccagcaattg cttacggcta
    39661 agattcgccg caaggtaagg gcgatgcaga cgcacaggca gtcgcggctg gcgcgttaac
    39721 aacacattga gattaggcca gtgggaaagt tcgttcatcc actcaacgct taaacgcggc
    39781 ataatcaacg agcgcagcaa aaatttctgg cgaaaactac ggcgatgcca gaatttaccc
    39841 ggccgacact gtccacgtgc cagactaaga aaaagtgaca ggctgctgag agattcagat
    39901 ggcgtaaagg tccgttcagt tagctgcgac atattcatga aatcattggt tatacatgac
    39961 gtcgatttca ccattgcgta tcttaaccaa acatcaatag tgtgattact aacgtaaatt
    40021 ttagggtttt gttgatattt cgttgaagtt aatgacccgg attggcatat ggagtattca
    40081 gaatatttat gaaaaagcgg aaaaccgtga agaagcgtta cgttattgcg ctggtgatag
    40141 tcatcgccgg actgattacg ttatggagaa ttcttaacgc acccgtgccg acttatcaga
    40201 cactgattgt gcgccccggt gatttacagc aaagcgtgct ggcgaccgga aagctggacg
    40261 cgctgcgtaa ggttgatgtg ggcgcgcagg tcagcggtca gttgaaaact ctgtcggtgg
    40321 cgattggcga taaagtaaaa aaagaccagc ttttaggggt tattgatcct gaacaggctg
    40381 aaaaccagat taaggaggtc gaagcaacgc tgatggagct acgtgcgcag cggcagcagg
    40441 cggaagcgga gctgaaactg gcgcgggtga cgtattcccg tcagcaacgt ctggcacaaa
    40501 cgcaggcggt ttcacagcag gatctcgaca ccgccgcgac ggagatggct gtgaaacagg
    40561 cgcaaattgg caccattgac gcggaaatca agcgcaatca ggcttctctc gatacggcta
    40621 aaaccaatct cgattacact cgcatcgttg ccccgatggc cggggaagtc acgcaaatca
    40681 ccactctgca aggccagacg gtgattgccg cacaacaagc accgaacatt ctgacgctgg
    40741 cagatatgag caccatgctg gtaaaagcgc aggtttctga agcggatgta atccacctga
    40801 agccggggca aaaagcttgg tttacggtgc ttggcgatcc actgacgcgc tacgaggggc
    40861 aaatcaagga tgtactaccg acgccggaaa aggttaacga cgctattttc tattacgccc
    40921 gttttgaagt ccccaacccc aatggtttgc tgcggctgga tatgactgcg caagtgcata
    40981 ttcagctcac cgatgtgaaa aatgtgctga cgatccctct gtcggcgtta ggcgatccgg
    41041 ttggcgataa tcgttataaa gtcaaattgt tgcgtaatgg tgaaacacgc gagcgtgaag
    41101 tgacgattgg cgcacgtaac gataccgatg ttgagattgt caaagggctg gaagcgggcg
    41161 atgaagtggt gattggtgag gccaaaccag gagctgcaca atgacgcctt tgctcgaatt
    41221 aaaggatatt cgtcgcagct atcctgccgg tgatgagcag gttgaggtgc tgaagggcat
    41281 caccctcgat atttatgccg gtgagatggt agcgattgtt ggcgcttcag gttccggtaa
    41341 atcgaccctg atgaatattc tcggctgtct ggataaggcc accagcggca cctatcgcgt
    41401 cgccggtcag gatgttgcca cactggacgc cgatgcgctg gcgcaactgc gccgcgagca
    41461 tttcggcttt attttccagc gttaccattt actttcgcat ttaaccgccg agcagaacgt
    41521 tgaagtaccc gccgtctatg ccggtcttga gcggaaacag cggctgcttc gcgcccagga
    41581 gttactgcaa cgactggggc tggaagaccg tacagagtat tatccggcac agctttcggg
    41641 tggtcagcaa cagcgcgtca gcatcgcgcg ggcattgatg aacggcggtc aggtaattct
    41701 tgccgatgaa ccaaccggcg cactggacag ccattctggc gaagaggtga tggcgatcct
    41761 gcatcagctg cgcgatcgcg ggcatatggt gattatcgtc acccacgatc cgcaggtcgc
    41821 tgctcaggcc gagcgggtga tcgaaattcg cgacggcgaa attgtgcgca atcctcccgc
    41881 cattgaaaaa gtgaatgttg ctggcgggac ggagcctgtt gtcaacacgg tgtctggctg
    41941 gcggcagttt gtcagcggtt ttaacgaggc gctgacgatg gcatggcggg cgctggcagc
    42001 gaataaaatg cgtactttac tgaccatgct ggggattatt atcggtattg cgtcggtggt
    42061 ttccattgtc gtggtgggtg acgccgccaa acaaatggtg ctggcggata ttcgttctat
    42121 tggtacgaat actattgatg tctatcccgg gaaagatttt ggcgatgacg atccgcaata
    42181 tcagcaggcg ctgaagtacg acgacttaat cgccatccaa aaacaaccgt gggtcgcctc
    42241 agccacacct gccgtctcgc aaaacctgcg cctgcgttat aacaatgttg atgttgctgc
    42301 cagtgccaat ggcgtgagcg gcgattattt taatgtctat ggcatgacct tcagtgaagg
    42361 aaacaccttt aatcaggagc agctgaacgg tcgtgcgcag gtcgtggttc tcgacagtaa
    42421 tactcgccgc cagcttttcc cccataaagc agatgtggtt ggcgaggtga ttctggtcgg
    42481 caatatgccc gccagagtca ttggtgtggc ggaagaaaaa cagtcgatgt ttggtagcag
    42541 taaagtgctg cgtgtctggc taccttacag cacgatgtcc gggcgagtta tgggccagtc
    42601 gtggctgaac tccattactg tcagggtaaa agaaggattt gacagcgccg aggcggaaca
    42661 gcaactcacg cgtttacttt cactgcgcca cggaaagaag gatttcttta cctggaacat
    42721 ggacggcgtc ttgaaaactg ttgaaaagac cacacgtact ttacaactgt ttctgacgct
    42781 ggtggcggtg atttcgctgg tggtgggcgg tattggtgta atgaatatta tgctggtgtc
    42841 agtgaccgag cggacgcggg aaattggcat tcgcatggct gtaggtgcgc gagcaagcga
    42901 tgttttgcaa cagttcctga tcgaagccgt actggtttgc ttggtcggtg gcgcgttggg
    42961 aataacgctg tcactgttaa ttgctttcac cttgcagctt ttcttacccg gctgggagat
    43021 tggtttttca ccgttggcac tgctgctggc gtttctctgc tcgacggcca ccgggatttt
    43081 atttggatgg ttacccgcac gaaatgcggc acgactggat ccagtagatg ctctggtacg
    43141 agagtaattt ttgagataaa aatgccagcc gatcgggctg gcattttgcc tttaggatgt
    43201 acacaatgag acagaagagc tatgcgactg ccgcttctac ttcgacgggc acaataacac
    43261 tggcgtgatt gccttttggc ccctggtgga catcaaactg aacggattgt ccagctttta
    43321 gcgttctgta accatccatc tgaatggtgg aataatgagc gaaaatatct tcgccgccgc
    43381 cttcagggca gatgaaacca aaccctttgg cattgttgaa ccacttaaca gtaccctttt
    43441 ccatgcttcg acatccttcg caaatcttat acaagtaaga tggaataaac cggggtcaga
    43501 gagggggctg ttcaaaacct cgccaactct agaaatacaa tttagagaat tagggcgagc
    43561 cgtcaagcgt ttgacagggg ccagggggca ggtatgaatc aaaaatttga agcagttaac
    43621 gctattgaca ggaatgtgac agatgtcgct gatgccaacg atagatgata gttatctatc
    43681 atgtggagta gattggtcag gcaaataagc tcttgtcagc ggcagggcgt tctgccgata
    43741 accgtaaccg aagatgataa ctgacaatgg gtaaaacgaa cgactggctg gactttgatc
    43801 aactggcgga agaaaaagtt cgcgacgcgc taaaaccgcc atctatgtat aaagtgatat
    43861 tagtcaatga tgattacact ccgatggagt ttgttattga cgtgttacaa aaattctttt
    43921 cttatgatgt agaacgtgca acgcaattga tgctcgctgt tcactaccag gggaaggcca
    43981 tttgcggagt ctttaccgcc gaggttgcag aaaccaaagt ggcgatggtg aacaagtacg
    44041 cgagggagaa tgagcatcca ttgctgtgta cgctagaaaa agcctgaatg caggcataaa
    44101 aattggggga ggtgcctatg ctcaatcaag aactggaact cagtttaaat atggctttcg
    44161 ccagagcgcg cgagcaccgt catgagttta tgaccgtcga gcacttgtta ctggcgctgc
    44221 tcagtaaccc atctgcccgg gaggcgctgg aagcgtgttc tgtggatttg gttgcgctcc
    44281 gtcaggaact ggaagccttt attgaacaaa ccacacccgt tctgcctgcc agtgaagagg
    44341 agcgcgacac acagccgacg ctgagttttc agcgtgtact gcaacgtgcg gtcttccatg
    44401 tccagtcctc cggtcgcaat gaggtaaccg gtgcaaacgt tctggtcgct atctttagcg
    44461 aacaggagtc gcaggcggca tatctgttgc gtaaacatga agtcagccgt ctcgatgtgg
    44521 tgaactttat ctctcatggc acgcgtaaag acgagccgac acagtcttct gatcctggca
    44581 gccagccaaa cagcgaagaa caagctggtg gggaggaacg tatggagaat ttcacgacga
    44641 acctgaatca gcttgcgcgc gtgggcggaa tcgacccact gattggtcgt gagaaggagc
    44701 tggagcgtgc tattcaggtt ctctgccgtc gccgtaaaaa caacccgctg ctggtggggg
    44761 aatctggtgt cggtaaaacc gcgattgcgg aaggtcttgc ctggcgaatt gttcagggcg
    44821 atgtgccgga agtgatggct gactgtacga tttactctct cgatatcggt tctctgttag
    44881 cgggcactaa atatcgcggc gactttgaaa aacgttttaa agcgttgctc aagcagctgg
    44941 agcaggacac taacagcatc ctgtttattg atgagatcca caccattatc ggtgcgggtg
    45001 cagcgtctgg tggccaggtc gatgcggcta acctgatcaa accgttgctc tccagcggta
    45061 aaattcgcgt aattggttcg acaacctatc aggagttcag caacattttc gagaaagacc
    45121 gtgctctggc gcgtcgcttc cagaaaattg atattactga accgtcgatc gaagaaactg
    45181 ttcaaatcat caatggcctg aaaccgaagt atgaagcgca ccacgacgtg cgttataccg
    45241 caaaagcggt gcgtgcagcg gtagagctgg cggtgaaata cattaacgat cgtcatctgc
    45301 cggataaagc cattgacgtt atcgacgaag cgggcgctcg cgcacgcctg atgccggtaa
    45361 gcaaacgcaa gaaaaccgtt aatgtggcgg atattgagtc cgtggtggcc cgtattgcgc
    45421 gcattccaga gaagagtgtt tctcagagtg accgcgatac cctgaaaaac ctcggcgatc
    45481 gcctgaaaat gctggtcttc ggtcaggata aagccattga ggcgctgact gaagccatta
    45541 agatggcgcg tgcaggttta ggtcacgaac ataaaccggt tggttcgttc ctgtttgccg
    45601 gccctaccgg ggtcgggaaa acagaggtga cggtacagct ttcgaaagcg ttgggcattg
    45661 agctgctgcg ctttgatatg tccgagtata tggaacgcca taccgtcagc cgtctgattg
    45721 gtgcgcctcc gggatacgtt ggttttgatc agggaggttt gctgactgat gcggtcatca
    45781 agcatccaca tgcggtgctg ttgctggacg aaatcgagaa agcgcatccg gacgtgttca
    45841 atattctgtt gcaggtgatg gacaacggta cgctgaccga taacaacgga cgcaaagcgg
    45901 acttccgtaa cgtggtgctg gtgatgacca ccaacgccgg ggtacgtgaa actgagcgta
    45961 aatccattgg tcttatccac caggataaca gcaccgatgc gatggaagag atcaagaaga
    46021 tctttacacc ggaattccgt aaccgtctcg acaacattat ctggttcgat catctgtcaa
    46081 ctgacgtgat ccatcaggtg gtggataaat tcatcgtcga gttgcaggtt cagctggatc
    46141 agaaaggtgt ttctctggaa gtgagccagg aagcgcgtaa ctggctggcc gagaaaggtt
    46201 acgaccgggc aatgggcgca cgtccgatgg cgcgtgtcat ccaggacaac ctgaaaaaac
    46261 cgctcgccaa cgaactgctg tttggttcgc tggtggacgg cggtcaggtc accgtcgcgc
    46321 tggataaaga gaaaaatgag ctgacttacg gattccagag tgcacaaaag cacaaggcgg
    46381 aagcagcgca ttaatctgat tgttaggtag gttggtcaag tccgtaatct cgaaagaggt
    46441 tacggacttt ttgtttatgg ggtggaggag gttcagaccc tttttttaat gatgatggta
    46501 agttgttgat aattagtgct gcgggaaggt aaggacaaaa aagggtgctg caggagaatg
    46561 ggatggtttt gctttattta caacgggcta aacgtgtagt atttgagttc actgccgtac
    46621 aggcagctta gaaattcaca ggtaacatac tccacccgcc caccatgttc actgccgtac
    46681 agacagataa aatgcgaaaa aaaagctcgc actttcgtac gagctcttct ttaaatatgg
    46741 cggtgagggg gggattcgaa cccccgatac gttgccgtat acacactttc caggcgtgct
    46801 ccttcagcca ctcggacacc tcaccaaatt gttttgctac caaacctcat gggtggcaac
    46861 ggggcgctac tatagggagt tggagtaaaa cggtcaagaa gaattttaat gataattatt
    46921 gtttgctcat actgtaaaca agttgtgcag tatatctaca tcgagacaag ttacggactt
    46981 atacttccaa agtacttcat acatatcaca aaataaaaag gccggttaaa ccgacctttt
    47041 actcgttctt tctcttcgcc catcaggcgg taaaacaatc agcgactacg gaagacaatg
    47101 cggcctttgc tcaggtcgta cggggtcagt tcaacagtca ctttgtcgcc cgtcaggatg
    47161 cggatgtagt ttttgcgcat tttaccggag atgtgtgcag taaccacgtg accgttttct
    47221 aactctacgc ggaacatggt attaggcaac gtttcaagaa cggtaccttg catttcaata
    47281 ttgtcttctt tggccatcta atcctctggg gtatcactac cgtaatttga accggcaaga
    47341 taatgccgaa gttctgtaaa taagtaaaga tttgcgcgct aaatcgcaac aaacaggttc
    47401 ggcacattac tccgaaaaca cacggctaag ccgcaccaaa agcgcaacgt ataagggagc
    47461 ggtgagataa acgatgggcg ttacctgacg cgaaaaattc cttatcggca gcggggtaat
    47521 gagcgtaacc aactctgcga ccgcaattat aacactctgg ggagaaatgt gccgaaaaca
    47581 ttcattcttg tggtgaaaac aagcatcgtg gtacccagaa attattcggc aatcgtccga
    47641 ggcgcatttg attgagataa ttaaggtaat cccggcgggg aatttcgcag gcaccaagcg
    47701 atgctgtgtg atcgttaagg acctggcagt cgataagctt accgccatga ccgataaact
    47761 cctcacagaa taccagaagc gccgttttag acgcattttc catccggctg aacatggact
    47821 cgccacaaaa tagcgttccc tgggccacgc cgtacatacc gccgacaagc tcatcttcac
    47881 gccagacttc aatggagtgg gcatggccga gttcgtgaag gcgatggtag gcttcgacca
    47941 cgccacgcgt gatccaggtt ccttcttcgc gatcgctggc acagccttca atgacctgac
    48001 caaaagcgta attcatcgtg acacgatagg gcgagcgttt atgaaatcgc ttcatactac
    48061 ggctgatatg cagtgattct ggccatagca ccgcgcgggg atcgggcgac caccagagga
    48121 tggggtcgcc tggagaaaac cacggaaaaa taccacgctg gtaagccatt aacaggcgcg
    48181 cagggctaag atcgccccca agtgccagca ggccgttagg ctcacgtaat gcgccttccg
    48241 gggaagggaa ggctattgaa tggcgagaaa gctgaaccag gcgcatgacc gcaaaactcc
    48301 acgcaagtcg gatcgttcaa taatagctta caaaccctgc ttgaactggt aataacgccc
    48361 ctgtctggca agcagctctg cgtgagtacc ttgctcaata atttgcccgt tgtccatcac
    48421 tattatttgt tggaaacgag agagtccgcg aagtcgatgg gtgaccatta acaccgtttt
    48481 ctcacgcatc atttctgcaa gcaattcaag gatctggctt tcggttgtgg catctaagcc
    48541 ttcggtaggt tcatccagca acaccagtgg cgcatcatgt aacagcgcac gggcgatagc
    48601 cagacggcgc agttcaccac cggagagctg gcgtccgcct tcacctaacc aactgttgag
    48661 acctgcatcc tcgagcagct tctccagacc aacgcgacgc aagctctccg ccagagcctc
    48721 atcactactg ccaggcgagg cgagtaacag attatcacgc agcgtggcgc taaacagatg
    48781 cactcgctga ggaacaacgc tgatggtctg tcgtagagcc gcttcattca ggctggctat
    48841 ggggctatcg ttaagcaaaa tctcgccctg ttgcgggtcc caagcgcggg tcagcagttg
    48901 taacagtgtt gatttgccgc atccggttcg cccgagaatc gcaatatgtt ccccggcgtt
    48961 tacctgaaga gaaatccctt taagtgcctg ttgggattgc tccggataag tgaactgaac
    49021 atcccgtaat gtcagcgaaa cgcgatcggc aacacgagtt tgggtatcag ggaaggtgac
    49081 ttccggtttt tgatccgtta aatcagtgat ccgaacggca gaggcaatga cttgccccag
    49141 atgctgaaat gcacccgtta ctggtgccag tgcttcaaac gcggctaacg cgcagaagac
    49201 aaacagggca attaacgcgc cgggttgagc attgccgcca acgccgccag acgccatcca
    49261 cagcatcagg atcaccgcta acgcgccaat gagcagcatt atcgcttgcg acaatgcggt
    49321 cagttcagat tgacggcgtt gcgcttccag ccattgaatt tctgtattct ctagttgcgt
    49381 gcgataacga tcgctggcac caaaaatggt cagctcagct tgcccttgca gccaggccgt
    49441 cagttgttgg cgatactgtc cgcgaagatg agtcagattt tgcccggtgc tttttcccgc
    49501 acgataaaac agcggtggca tcaggaaaag cgtcagtaac ataatgccgc ccagcgtaaa
    49561 ggcgagggtg aaatcaagga aacttaaccc gattgtcacc atcataatca ccacaaaagc
    49621 gcccaccagc ggcgagataa cgcgcaggta aagatgatcg agcgtatcaa catccgccac
    49681 cacgcgattg agcaattcgc cctgacgata gcgcgccagt ccggcagggg agaggggcaa
    49741 caatttgctg aaggtgtaaa tgcgcagatg ctgcaacacg cggaaagtcg cgtcgtgact
    49801 taccagacgt tcaaaataac gcccggcagt acgggtgatt gcggcaccac gcacgcccgc
    49861 agcgggtagc atatagttga agctgtacag tccggcaacc cccgcaaccg ctgaggccga
    49921 gaggaaccag ccggaaagtg tcaacagtcc gatactggcg agcagcgtca caattgccag
    49981 cacaatacca agacttaaca tccatttatg acgtttatac agtgctagat agggtagcaa
    50041 agcgcgcatt taaatctcct cctgacgatg ggccagtaat gtggcgaatg ggccaccagc
    50101 cacacttaat tccgcgtaac gtccttgctc aataatccgg ccatcctgca taacccaaat
    50161 gacatcccag tcagcaagat cttctaactg gtgggtgacc attaacgttg tctggcgcag
    50221 agaggcggca ttcagcgcct ccattacgcg ctgttcactg tgagcatcaa ggctggcagc
    50281 gggttcatcc aacagtaata gcgaacaggg atttagtaac gcacgggcca ccgccacgcg
    50341 ctgcgcctgc cccacggaaa ggcgggcagc ctggtcgcca acaggcgtat caacgccttg
    50401 tggcaggagc ggtagaaact cgctgaccca ggcgttatcc agcgctgctt gtaattcatg
    50461 ttcgctggca tcaggtcgcg ccagtagtac gttatcccgt aatgttgctg ccggtaattg
    50521 tggattttgc ccaacccagg agagatgttt acgccatgat tctggtgata aatcgcgtaa
    50581 ttctatcccg ttgattcgta gcgatccctg atatgagaga aaaccagaaa gcgcgttcaa
    50641 cagtgaactt ttacctgaac cgctgcgacc aaccaacacg gcacgttggc ctgctggcaa
    50701 agtaaagttc agcggtccgg ccagcgtttt accttccggc gacgtgataa acagatcctc
    50761 ggcctcaatg gtcaccggat cggtcgatgc taattccacc tcgccacact gcggatgggc
    50821 gagcggggtt tccataaacg ttttcagact gtcagctgca ccaacagcct gggctttagc
    50881 atgataaaac gtaccgagat cgcgtaatgg ctggaaaaac tctggcgcaa ggatcagggc
    50941 cagaaaaccc gcagccagcg tcacgccagt atcgtagtga ccaaaatcca gctcgccaag
    51001 ataggaaaaa ccaaagtaga ccgccaccag agcaattgac agcgaggtaa aaaattcgag
    51061 aatgccggag gataaaaatg ccagccgtag cacttccatt gtccgttggc ggaaatcttc
    51121 cgaagcagaa cgaatacttt caatttcagc ttcaccacga ccaaaaatac gcaatgtttc
    51181 catgccgcgc aggcgatcga ggaaatgccc acttaagcga gcaagagcga gaaagttacg
    51241 tcggttagca tcggcagccc ccattccaac cagcgccata aacaacggaa ttaacggtgc
    51301 agtgcccagc agaatgagcg ccgcagccca gtttgagggg aagattgcca ccacaatcag
    51361 caacggcacc gacactgcca gcgccatttg cggcaggtag cgtgcatagt aatcatgcat
    51421 atcgtcaatt tgctcgagta ccagcgtcgc ccagctcccc gcaggtttac cctgaatcca
    51481 cgctggccct gcttgttgca gacggtcgag aacctgccgg cggatggcaa agcggatatg
    51541 ctgcccggcg tgataaccca cccgttcgcg taaccagacc acccatgcgc gcagtacaaa
    51601 ggtcagaacc agtaacgtaa agggaagcag cagggcttca cggggaatat tctccataat
    51661 catatgttgc agaattcgcg ccatgaacca ggcctgggca atgatcaata tgccgctcac
    51721 aaagcccagc agacgagaaa tattcagcca acgttgggag atgacgcttt gctgttttaa
    51781 ccagcgggtt aactcttttt gacgagattt attcattgca cgcttagcag gtgagttatc
    51841 agaattattt gcagagcaat gttacaacgg ggaaaaaata aaggcgaccc atagtcgcaa
    51901 ggtgtcgcct tctttacttt tgttactgat ttgtaaaatt attttgcgtc agctaaacca
    51961 tcgaggtagc gttccgcatc aagtgctgcc atgcagcctg taccggccga agtaatggcc
    52021 tgacgataaa tatgatccat cacgtcaccc gcggcaaaga cgccaggaat gctggtctgg
    52081 gtggcattac catgaatacc cgactgtact ttgatgtagc cgttttccag ttccagctgc
    52141 ccttcgaaaa tggcagtatt cgggctgtga ccgatagcaa caaacagacc ggcaacgtcg
    52201 agtgattcga tgttatcgct gttttgcgta tcgcgcagac gaacgccagt gacacccatt
    52261 tgatcgccgg tcacttcttc cagcgtccgg ttggtgtgca gaatgatgtt gccgttctcc
    52321 actttatcca tcagacgctt aatgaggatt ttttccgcgc ggaaaccgtc acggcggtga
    52381 atcagatgta cttccgaagc gatgttagac agatacaacg cctcttcaac cgcggtattg
    52441 ccgccgccga tgaccgcaac tttctggttg cgatagaaga aaccgtcgca ggttgcacaa
    52501 gcagaaaccc cacggccttt aaaggcttct tcagagggca ggccgagata gcgtgcagaa
    52561 gctccggtgg caataatcag cgcgtcgcaa gtgtattcgc cgttatcgcc attcagacgg
    52621 aacggacggt tttgcagatc caccttgttg atatgatcaa aaatgatttc agtttcaaat
    52681 ttggtggcat gttcgtgcat gcgctccatt aataacggac cggtcagatc gtttggatcg
    52741 ccaggccagt tttccacttc tgtggtggtg gtcagttggc cgcctttttc catgccggta
    52801 atcagcacag gttgcaggtt ggcgcgcgcc gcatagacag cagcggtgta tcccgccggg
    52861 cctgaaccca ggataagcag tttactgtgt ttggtcgtgc ccatgagatc cccatagttg
    52921 ttggcagaca atgggcagga ttgtagggaa tttactgacg taaaaaaaga gtatgacgat
    52981 tttgttaaca atttgtgcaa tcggcagcat cgataagcag gtcaaattct cccgtcatta
    53041 tcacctctgc tacttaaatt tcccgcttta taagctgatt aaatgatgaa taaacgcccc
    53101 tgttaatgaa tatctggcat gttgtactaa aaatcgatgt tttgctttga caatcccctg
    53161 gtgttttgcg aaaacattcg aggaagaaaa aaaacagtat tcttatatgc gcataaccat
    53221 gcatgtaaat accatgttta ccgtgctagt gaaatctacg tatggcgtgg acagacgcca
    53281 ttcgtgatgt cgatagctgc cacaaggcaa cggtcttctc accgtagacc caggcattgc
    53341 gcgccgtgaa tcttcatgat ttcggtctat cgtgacgggt agcgactctg aacagtgatg
    53401 tttcagggtc agacaggagt agggaaggaa tacagagaga caataataat ggtagatagc
    53461 aagaagcgcc ctggcaaaga tctcgaccgt atcgatcgta acattcttaa tgagttgcaa
    53521 aaggatgggc gtatttctaa cgtcgagctt tctaaacgtg tgggactttc cccaacgccg
    53581 tgccttgagc gtgtgcgtcg gctggaaaga caagggttta ttcagggcta tacggcgctg
    53641 cttaaccccc attatctgga tgcatcactt ctggtattcg ttgagattac tctgaatcgt
    53701 ggcgcaccgg atgtgtttga acaattcaat accgctgtac aaaaacttga agaaattcag
    53761 gagtgtcatt tagtatctgg tgatttcgac tacctgttga aaacacgcgt gccggatatg
    53821 tcagcttacc gtaagttgct gggggaaacc ctgctgcgtc tgcctggcgt caatgacaca
    53881 cggacatacg ttgttatgga agaagtcaag cagagtaatc gtctggttat taagacgcgc
    53941 taacacggaa caggtgcaaa atcggcgtat tttgattaca ctcctgttaa tccatacagc
    54001 aacagtactg gggtaacctg gtactgttgt ccgttttagc atcgggcagg aaaagcctgt
    54061 aacctggaga gcctttcttg agccaggaat acactgaaga caaagaagtc acattgacaa
    54121 agttaagtag cggccgtcgc cttctggaag cgttgctgat ccttattgtc ctgtttgccg
    54181 tctggttgat ggctgcctta ctaagcttta acccttcgga ccccagctgg tcgcaaacgg
    54241 cctggcatga acctatccat aatttaggtg ggatgcccgg tgcgtggctg gcagatacgc
    54301 tgttctttat ttttggcgtg atggcttaca ccattcccgt cattattgtc ggcggttgtt
    54361 ggtttgcctg gcgtcatcag tccagtgacg aatacattga ttattttgcc gtttcgttac
    54421 gcatcattgg cgttttggcg ctcatcctta cctcctgtgg tctggcggca atcaacgctg
    54481 acgatatctg gtattttgcc tccggtggcg tcattggcag cttactaagc actacgttac
    54541 aaccactgct acacagtagc gggggaacta ttgcgctgct ctgcgtttgg gctgcgggcc
    54601 tgacgctgtt caccggttgg tcatgggtga ccattgctga aaaactcggc ggctggattt
    54661 taaacattct caccttcgcc agtaatcgta cccgtcgcga tgatacctgg gtcgatgaag
    54721 atgaatatga agatgacgaa gagtatgaag atgaaaacca cggcaaacag catgaatcac
    54781 gccgtgcccg tattcttcgc ggcgcgctag cgcgtcgtaa acggttggcg gaaaaattca
    54841 ttaatccgat ggggcggcaa acagacgctg cgttgttctc cggtaagcgg atggatgatg
    54901 aagaagagat tatctacact gcacgcggtg tggctgccga cccggacgac gtcctatttt
    54961 cgggcaatcg tgcaacgcag ccagaatatg acgaatacga tccattatta aacggtgcgc
    55021 caattaccga acctgttgct gtagcagctg ctgctaccac ggcgacacaa agctgggctg
    55081 cgccggttga acctgtgacc cagacgccgc ctgttacttc tgttgatgtt ccaccttcgc
    55141 aacctacagt agcctggcag cctgtaccgg gtccacaaac gggagagccg gttattgctc
    55201 ctgcaccgga aggttaccca cagcagccac aatatgcgca gcctgcagtg caatataatg
    55261 agccgctgca acaacctgta cagccacagc agccgtatta tgcgcctgca gctgaacaac
    55321 ctgtgcaaca accttactac gctcctgcgg cggaacaacc tgttcagcaa ccgtattacg
    55381 caactgcgcc ggaacagcct gcgcaacagc cgtattatgc ccctgcgcca gaacaaccgg
    55441 tggcaggtaa cgcctggcaa gccgaagagc agcaatccac ttttgctcca cagtctacat
    55501 accagactga gcaaacttat cagcagccag ccgctcagga gccgttgtac caacagccgc
    55561 aacccgttga acagcagcct gttgtggagc ctgaacccgt tgtagaagag acaaaacccg
    55621 cgcgtccgcc gctttactat tttgaagaag tggaagagaa gcgagcccgt gaacgtgaac
    55681 aacttgcggc ctggtatcaa ccgattccag aaccggttaa agaaccagaa ccgatcaaat
    55741 cttcgttgaa agcgccttct gctgcagcag tacctccagt agaagccgct gccgctgttt
    55801 ctccgctggc atctggcgtg aaaaaagcga cactggcgac gggggctgcc gcaaccgttg
    55861 ccgcgccagt cttcagtctg gcaaatagcg gtggaccgcg tccgcaagtc aaagagggaa
    55921 ttggcccgca gttgccacga ccgaaacgta ttcgcgtgcc aactcgtcgt gaactggcgt
    55981 cttacggtat taagctgccc tcacagcgtg cggcggaaga gaaagcccgt gaagcccggc
    56041 gcaatcagta tgattctggc gatcagtata acgatgatga aatcgatgcg atgcagcagg
    56101 atgaactggc acgtcagttc gcccagacac agcagcaacg ctatggcgaa cagtatcaac
    56161 atgatgtgcc cgtaaacgca gaagatgcag atgctgcggc agaggctgaa ctggctcgtc
    56221 agtttgcgca aactcaacaa caacgttatt ccggcgaaca accggctggg gcgaatccgt
    56281 tctcgctgga tgattttgaa ttttcgccaa tgaaagcgtt gctggatgat ggtccacacg
    56341 aaccgttgtt tacgccaatt gttgaacctg tacagcagcc gcaacaactg gttgcaccgc
    56401 agcagcagta tcagcagcca caacagccgg ttgcgccaca gcagcaatat cagcagccgc
    56461 aacagccggt tgcgccgcag ccgcagtatc agcagccaca acagcaggtt gcgccgcagc
    56521 cacaatatca gcagccgcaa caaccggttg caccgcagca gcaggatacc ctgcttcatc
    56581 cgctgttgat gcgtaatggc gacagccgtc cgttgcataa accgacgacg ccgctgcctt
    56641 ctctggattt gctgacaccg ccgccgagcg aagtggagcc ggtagatacc tttgcgcttg
    56701 aacaaatggc tcgcctggtg gaagcgcgtc tggctgattt ccgtattaaa gccgatgtcg
    56761 tcaattactc tccggggccg gttatcactc gctttgaact taacctggct cctggcgtaa
    56821 aagcggcgcg catttctaac ttgtcacggg accttgcccg ttcactttcg acggtggcgg
    56881 tgcgtgtcgt tgaagttatt cctggcaaac cctatgtagg tctggagtta ccaaataaaa
    56941 aacgacaaac cgtttatctg cgcgaagttt tggataacgc caaattccgc gataatccgt
    57001 cgccattaac cgtggtgctg ggtaaagata tcgccggtga gccggtggtt gccgatctgg
    57061 cgaaaatgcc gcacttgttg gttgcgggga ctaccggttc cggtaaatct gtcggtgtga
    57121 acgcgatgat cctgagcatg ctttataaag cacagccaga agatgtgcgt ttcatcatga
    57181 tcgacccgaa aatgctggag ctttcggttt atgaaggcat tccgcatctg ttaacggaag
    57241 tcgttactga tatgaaagat gccgccaacg cgctgcgctg gtgtgttaac gagatggagc
    57301 gtcgttataa actgatgtct gcgctgggtg tgcgtaatct ggcgggttat aacgaaaaaa
    57361 ttgctgaagc cgatcgcatg atgcgtccga ttccagaccc gtactggaag ccgggtgaca
    57421 gtatggatgc ccagcatccg gtgctgaaaa aagaaccata cattgtggtg ttggttgacg
    57481 aatttgccga cctgatgatg acggtaggta aaaaagtgga agagctgata gcacgtctgg
    57541 cgcaaaaagc ccgtgccgcg ggtatccacc tcgtactggc aactcagcgt ccatcggttg
    57601 atgttattac tggtctgatt aaagcgaata ttccgacccg tatcgccttt accgtatcca
    57661 gtaagattga ctcacgtacc attcttgatc aggctggcgc ggaatcactg ctggggatgg
    57721 gggatatgct ctactctggg ccgaactcca cgttgccggt acgtgtccat ggtgcttttg
    57781 ttcgcgatca ggaagttcat gccgtggtgc aggactggaa agcgcgtggt cgcccacagt
    57841 atgttgatgg catcacttcc gacagtgaaa gcgaaggtgg cgcgggcggt ttcgatggcg
    57901 ctgaagaact ggatccgttg ttcgatcagg cggtgcagtt tgtcactgaa aaacgcaaag
    57961 cgtcaatttc tggcgtacag cgtcagttcc gcattggtta taaccgtgca gcgcgtatta
    58021 tcgaacagat ggaagcgcag gggattgtca gcgaacaggg gcacaacggt aatcgtgaag
    58081 tgctggcccc accgccgttt gactaattaa tgcattgccg gataaggcgc ggtagcgtcg
    58141 catccggcac tctatcaact gaaaattcag tattttcttt tttcctcaag ctgattatta
    58201 gcctggaata gagagtagag ggaactcccg atcgggagtg acgtaatttg aggaataatg
    58261 atgaaaaaaa ttgccatcac ctgtgcatta ctctcaagct tagtagcaag cagcgtttgg
    58321 gctgatgccg caagcgatct gaaaagccgc ctggataaag tcagcagctt ccacgccagc
    58381 ttcacacaaa aagtgactga cggtagcggt gcggcggtgc aggaaggtca gggcgatctg
    58441 tgggtgaaac gtccaaactt attcaactgg catatgacac aacctgatga aagcattctg
    58501 gtttctgacg gtaaaacact gtggttctat aacccgttcg ttgagcaagc tacggcaact
    58561 tggctgaaag atgccaccgg taatacgccg tttatgctga ttgcccgcaa ccagtccagc
    58621 gactggcagc agtacaatat caaacagaat ggcgatgact ttgtcctgac gccgaaagcc
    58681 agcaatggca atctgaagca gttcaccatt aacgtgggac gtgatggcac aatccatcag
    58741 tttagcgcgg tggagcagga cgatcagcgc agcagttatc aactgaaatc ccagcaaaat
    58801 ggggctgtgg atgcagcgaa atttaccttc accccgccgc aaggcgtcac ggtagatgat
    58861 caacgtaagt agaggcacct gagtgagcaa tctgtcgctc gatttttcgg ataatacttt
    58921 tcaacctctg gccgcgcgta tgcggccaga aaatttagca cagtatatcg gccagcaaca
    58981 tttgctggct gcggggaagc cgttgccgcg cgctatcgaa gccgggcatc tgcattctat
    59041 gatcctctgg gggccaccgg gtaccggcaa aacaactctc gctgaagtga ttgcccgcta
    59101 tgcgaacgct gatgtggaac gtatttctgc cgtcacctct ggcgtgaaag agattcgcga
    59161 ggcgatcgag cgcgcccggc aaaaccgcaa tgcaggtcgc cgcactattc tttttgttga
    59221 cgaagttcac cgtttcaaca aaagccagca ggatgcattt ctgccacata ttgaagacgg
    59281 caccatcact tttattggcg caaccactga aaacccgtcg tttgagctta attcggcact
    59341 gctttcccgt gcccgtgtct atctgttgaa atccctgagt acagaggata ttgagcaagt
    59401 actaactcag gcgatggaag acaaaacccg tggctatggt ggtcaggata ttgttctgcc
    59461 agatgaaaca cgacgcgcca ttgctgaact ggtgaatggc gacgcgcgcc gggcgttaaa
    59521 tacgctggaa atgatggcgg atatggccga agtcgatgat agcggtaagc gggtcctgaa
    59581 gcctgaatta ctgaccgaaa tcgcaggtga acgtagtgcc cgctttgata acaaaggtga
    59641 tcgcttttac gatctcattt ccgcactgca taaatcggta cgtggtagcg cacccgatgc
    59701 ggcgctgtac tggtatgcgc gaattattac tgccggtggc gatccgttat atgttgcgcg
    59761 tcgctgtctg gcgattgcgt ctgaagatgt gggtaatgct gatccacgag cgatgcaggt
    59821 ggcaattgca gcctgggatt gctttactcg cgttggcccg gcggaaggtg aacgcgccat
    59881 tgctcaggcg attgtttacc tggcctgcgc gccaaaaagc aacgctgtct acactgcgtt
    59941 taaagctgcg ctggcggatg ctcgcgaacg tccggattat gacgtgccgg ttcatttgcg
    60001 taatgcgccg acgaaattaa tgaaggaaat gggctacggg caggaatatc gttacgctca
    60061 tgatgaagca aacgcttatg ctgccggtga ggtttacttc ccgcgggaaa tagcacaaac
    60121 acgctattat ttcccgacaa acaggggcct tgaaggcaag attggcgaaa agctcgcctg
    60181 gctggctgaa caggatcaaa atagccccat aaaacgctac cgttaatgtt atcgttgcgg
    60241 taatgttgtt actgtatccc tgtggtcgca ggctgtggcc acatctccca tttaattcga
    60301 taagcacagg ataagcatgc tcgatcccaa tctgctgcgt aatgagccag acgcagtcgc
    60361 tgaaaaactg gcacgccggg gctttaagct ggatgtagat aagctgggcg ctctcgaaga
    60421 gcgtcgtaaa gtattgcagg tcaaaacgga aaacctgcaa gcggagcgta actcccgatc
    60481 gaaatccatt ggccaggcga aagcgcgcgg ggaagatatc gagcctttac gtctggaagt
    60541 gaacaaactg ggcgaagagc tggatgcagc aaaagccgag ctggatgctt tacaggctga
    60601 aattcgcgat atcgcgctga ccatccctaa cctgcctgca gatgaagtgc cggtaggtaa
    60661 agacgaaaat gacaacgttg aagtcagccg ctggggtacc ccgcgtgagt ttgactttga
    60721 agttcgtgac cacgtgacgc tgggtgaaat gcactctggc ctcgactttg cagctgcagt
    60781 taagctgact ggttcccgct ttgtggtaat gaaagggcag attgctcgca tgcaccgcgc
    60841 actgtcgcag tttatgctgg atctgcatac cgaacagcat ggctacagtg agaactatgt
    60901 tccgtacctg gttaaccagg acacgctgta cggtacgggt cagctgccga aatttgctgg
    60961 cgatctgttc catactcgtc cgctggaaga agaagcagac accagtaact atgcgctgat
    61021 cccaacggca gaagttccgc tgactaacct ggtgcgcggt gaaatcatcg atgaagatga
    61081 tctgccaatt aagatgaccg cccacacccc atgcttccgt tctgaagccg gttcatatgg
    61141 tcgtgacacc cgtggtctga tccgtatgca ccagttcgac aaagttgaaa tggtgcagat
    61201 cgtgcgcccg gaagactcaa tggcggcgct ggaagagatg actggtcatg cagaaaaagt
    61261 cctgcagctg ctgggcctgc cgtaccgtaa aatcatcctt tgtaccggcg acatgggctt
    61321 tggcgcttgc aaaacctatg acctggaagt atggatcccg gcacagaaca cctaccgtga
    61381 gatctcttcc tgctccaacg tttgggattt ccaggcacgt cgtatgcagg cacgttgccg
    61441 cagcaagtcg gacaagaaaa cccgtctggt tcataccctg aacggttctg gtctggctgt
    61501 tggtcgtacg ctggttgcag taatggaaaa ctatcagcag gctgatggtc gtattgaagt
    61561 accagaagtt ctgcgtccgt atatgaacgg actggaatat attggctaat acccaatttt
    61621 tctgaatcta aaaagcgcct gcgggcgctt tttttgtctc cctttgatac cgaacaataa
    61681 ttactcctca cttacacgta atactacttt cgagtgaaaa tctacctatc tctttgattt
    61741 tcaaattatt ctatgtatac aagcctatat agcgagctgc tatagaaata attacacaat
    61801 acggtttgtt actggaatca atcgtgagca agcttgagtg agccattatg aaaacgaaaa
    61861 tccctgatgc ggtattggct gctgaggtga gtcgccgtgg tttggtaaaa acgacagcga
    61921 tcggcggcct ggcaatggcc agcagcgcat taacattacc ttttagtcgg attgcgcacg
    61981 ctgtcgatag cgccattcca acaaaatcag acgaaaaggt tatctggagc gcctgtacag
    62041 ttaactgtgg tagtcgctgc ccgctacgta tgcacgtcgt ggacggtgaa atcaaatatg
    62101 tcgaaacgga caataccggc gatgacaatt acgacggcct gcactaggtt cgcgcctgcc
    62161 tgcgtgggcg ttccatgcgt cgccgtgtct acaatccgga ccgcctgaaa tatccgatga
    62221 aacgagtcgg ggcgcgcggt gaaggcaaat tcgagcgcat tagctgggaa gaagcctacg
    62281 acatcatcgc gaccaatatg cagcgcctga tcaaagagta cggcaacgag tctatctatc
    62341 tgaactatgg caccggtacg ctgggcggca ccatgacccg ctcctggccg ccgggaaata
    62401 ccctggtcgc gcggctgatg aactgctgcg gcggctatct gaaccattac ggcgactact
    62461 cctccgcgca aattgcggaa ggtttgaact atacctacgg cggctgggca gatggcaaca
    62521 gcccgtcgga tatcgaaaac agtaagctgg tagtgctgtt tggtaataac cctggcgaaa
    62581 cgcgaatgag tggcagtggg gtgacttact atcttgaaca ggcacgccag aaatctaatg
    62641 cccgcatgat catcatcgat ccgcgctata ccgacaccgg tgccgggcgc gaagatgagt
    62701 ggatccctat tcgtccggga acagatgccg cactggttaa cggtctggcg tacgtcatga
    62761 tcactgaaaa cctggtggat caggcattcc tcgataaata ttgcgttggc tacgatgaga
    62821 aaaccctgcc agccagtgcg ccgaaaaatg gccactataa agcttatatt ctgggtgaag
    62881 ggccagatgg cgtggctaaa acaccggaat gggcctcaca aatcaccggt gttccggcag
    62941 acaaaatcat caaactggct cgtgaaatcg gcagtaccaa accggcgttt atcagccagg
    63001 gatggggccc gcagcgtcac gctaacggtg aaatcgcaac ccgtgctatc tcgatgctgg
    63061 cgattctgac cggtaacgtt ggtattaacg gaggcaacag cggcgcgcgt gaaggttcat
    63121 acagcttacc gtttgtccgt atgccgacct tggaaaaccc gatccagacc agcatttcga
    63181 tgtttatgtg gaccgatgcc attgaacgtg gcccggaaat gacggcgctg cgtgatggtg
    63241 tacgcgggaa agataagctg gatgtgccga tcaaaatgat ctggaactat gccggtaact
    63301 gcctgattaa ccagcattct gaaatcaacc gtacccatga aatccttcag gatgataaga
    63361 agtgcgagct gattgtggtt atcgactgcc acatgacctc atcggcgaaa tatgctgaca
    63421 tcctgctgcc tgactgcacc gcttccgaac agatggactt tgcgctggat gcatcctgcg
    63481 ggaatatgtc ttacgtgatt ttcaacgatc aggtgattaa accgcgcttt gaatgtaaga
    63541 ccatctatga aatgaccagc gaactggcaa aacgtcttgg cgttgagcaa cagtttactg
    63601 aaggccgtac acaggaagag tggatgcggc atctgtatgc ccagtcgcgg gaagcgattc
    63661 ctgaactacc aacgtttgaa gagttccgca agcaggggat ctttaaaaag cgcgacccac
    63721 aagggcacca cgttgcttat aaagctttcc gtgaagatcc tcaggcaaat tcactgacca
    63781 cgccatcggg caaaattgag atttattcgc aggcgctggc tgacattgcc gctacctggg
    63841 aattgccaga aggagatgtg atcgatccac tgccgatcta cacgccgggc tttgaaagtt
    63901 atcaggatcc gttgaacaaa cagtatccgc tgcagcttac gggtttccac tataaatctc
    63961 gcgttcactc aacttacggc gacgttgatg tgctgaaagc agcttgccgt caggaaatgt
    64021 ggatcaaccc gcttgatgcg caaaaacgcg gtatcaacaa cggcgataaa gtcaggatct
    64081 ttaacgatct tggtgaggtt catattgagg cgaaagtgac gccacgaatg atgccgggtg
    64141 tggtcgcact gggtgaaggt gcctggtatg acccggatac aaaacgtgtc gataagggcg
    64201 gttgtatcaa cgtactgacc actcaacgtc cgtctcctct cgctaagggg aatccgtcac
    64261 atacaaacct tgttcaggtt gaaaaggtgt aaggagtaac cgatgacaac ccagtatgga
    64321 ttttttattg attccagccg ttgcaccggt tgcaaaacct gcgagctggc ctgtaaagac
    64381 tacaaagatt tgacgccaga agtcagcttc cgccgcattt atgaatatgc tggcggcgac
    64441 tggcaggaag ataac
//
LOCUS       DAJVUJ010000007        47217 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.007, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000007 DAJVUJ010000000
VERSION     DAJVUJ010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47217
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.007"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            27..764
                     /locus_tag="OQQ48_000452"
     CDS             27..764
                     /locus_tag="OQQ48_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCS1951336.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELRAGVVVGGTRF
                     IFPADRESISILLANTSQESWLINSKINRPTRWAGGKASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTSWCQ
                     AIAPCHVAWRAINDYDGLSAKKEQNLP"
     gene            875..1885
                     /gene="pyrD"
                     /locus_tag="OQQ48_000453"
     CDS             875..1885
                     /gene="pyrD"
                     /locus_tag="OQQ48_000453"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HCS1951337.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            2059..2601
                     /gene="zapC"
                     /locus_tag="OQQ48_000454"
     CDS             2059..2601
                     /gene="zapC"
                     /locus_tag="OQQ48_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HCS1951338.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNVLVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(2598..3707)
                     /gene="ycbX"
                     /locus_tag="OQQ48_000455"
     CDS             complement(2598..3707)
                     /gene="ycbX"
                     /locus_tag="OQQ48_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HCS1951339.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDRWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     ILEGEVSPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            3951..6059
                     /gene="rlmKL"
                     /locus_tag="OQQ48_000456"
     CDS             3951..6059
                     /gene="rlmKL"
                     /locus_tag="OQQ48_000456"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HCS1951340.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPLKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYSDWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGALSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            6071..7978
                     /locus_tag="OQQ48_000457"
     CDS             6071..7978
                     /locus_tag="OQQ48_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS1951341.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIVLIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNIAEGKQEVMVNGKPRHVLSYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKTEEAAAAKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            8108..9361
                     /gene="pqiA"
                     /locus_tag="OQQ48_000458"
     CDS             8108..9361
                     /gene="pqiA"
                     /locus_tag="OQQ48_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HCS1951342.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            9366..11006
                     /gene="pqiB"
                     /locus_tag="OQQ48_000459"
     CDS             9366..11006
                     /gene="pqiB"
                     /locus_tag="OQQ48_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HCS1951343.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENTDVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            11003..11566
                     /gene="pqiC"
                     /locus_tag="OQQ48_000460"
     CDS             11003..11566
                     /gene="pqiC"
                     /locus_tag="OQQ48_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HCS1951344.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSVQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            11822..11989
                     /gene="rmf"
                     /locus_tag="OQQ48_000461"
     CDS             11822..11989
                     /gene="rmf"
                     /locus_tag="OQQ48_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HCS1951345.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(12059..12577)
                     /gene="fabA"
                     /locus_tag="OQQ48_000462"
     CDS             complement(12059..12577)
                     /gene="fabA"
                     /locus_tag="OQQ48_000462"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HCS1951346.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(12646..14406)
                     /locus_tag="OQQ48_000463"
     CDS             complement(12646..14406)
                     /locus_tag="OQQ48_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HCS1951347.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            14592..15044
                     /gene="matP"
                     /locus_tag="OQQ48_000464"
     CDS             14592..15044
                     /gene="matP"
                     /locus_tag="OQQ48_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HCS1951348.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKLDLQALLGKE"
     gene            complement(15120..16160)
                     /gene="ompA"
                     /locus_tag="OQQ48_000465"
     CDS             complement(15120..16160)
                     /gene="ompA"
                     /locus_tag="OQQ48_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HCS1951349.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(16517..17026)
                     /gene="sulA"
                     /locus_tag="OQQ48_000466"
     CDS             complement(16517..17026)
                     /gene="sulA"
                     /locus_tag="OQQ48_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HCS1951350.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            17245..17874
                     /gene="sxy"
                     /locus_tag="OQQ48_000467"
     CDS             17245..17874
                     /gene="sxy"
                     /locus_tag="OQQ48_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HCS1951351.1"
                     /translation="MKSLSYKRIYKSQEYLAMLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(17837..19999)
                     /gene="yccS"
                     /locus_tag="OQQ48_000468"
     CDS             complement(17837..19999)
                     /gene="yccS"
                     /locus_tag="OQQ48_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HCS1951352.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGLLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGFWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(20009..20455)
                     /locus_tag="OQQ48_000469"
     CDS             complement(20009..20455)
                     /locus_tag="OQQ48_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HCS1951353.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            20578..22632
                     /gene="helD"
                     /locus_tag="OQQ48_000470"
     CDS             20578..22632
                     /gene="helD"
                     /locus_tag="OQQ48_000470"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HCS1951354.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGERCDLDTTYRFNSRI
                     GKVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(22664..23122)
                     /gene="mgsA"
                     /locus_tag="OQQ48_000471"
     CDS             complement(22664..23122)
                     /gene="mgsA"
                     /locus_tag="OQQ48_000471"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HCS1951355.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(23218..23880)
                     /locus_tag="OQQ48_000472"
     CDS             complement(23218..23880)
                     /locus_tag="OQQ48_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HCS1951356.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            24053..24466
                     /locus_tag="OQQ48_000473"
     CDS             24053..24466
                     /locus_tag="OQQ48_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HCS1951357.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(24511..24828)
                     /gene="hspQ"
                     /locus_tag="OQQ48_000474"
     CDS             complement(24511..24828)
                     /gene="hspQ"
                     /locus_tag="OQQ48_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HCS1951358.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(24886..26076)
                     /gene="rlmI"
                     /locus_tag="OQQ48_000475"
     CDS             complement(24886..26076)
                     /gene="rlmI"
                     /locus_tag="OQQ48_000475"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HCS1951359.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALVSALQTLYPECAIYDRSDVA
                     VRKKEGMELTLGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            26171..26449
                     /gene="yccX"
                     /locus_tag="OQQ48_000476"
     CDS             26171..26449
                     /gene="yccX"
                     /locus_tag="OQQ48_000476"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCS1951360.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(26446..26775)
                     /gene="tusE"
                     /locus_tag="OQQ48_000477"
     CDS             complement(26446..26775)
                     /gene="tusE"
                     /locus_tag="OQQ48_000477"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HCS1951361.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(26866..27525)
                     /gene="yccA"
                     /locus_tag="OQQ48_000478"
     CDS             complement(26866..27525)
                     /gene="yccA"
                     /locus_tag="OQQ48_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HCS1951362.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIVHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(27732..27819)
                     /locus_tag="OQQ48_000479"
     tRNA            complement(27732..27819)
                     /locus_tag="OQQ48_000479"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27783..27785),aa:Ser,seq:tga)
     gene            28246..29364
                     /gene="hyaA"
                     /locus_tag="OQQ48_000480"
     CDS             28246..29364
                     /gene="hyaA"
                     /locus_tag="OQQ48_000480"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HCS1951363.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            29361..31154
                     /gene="hyaB"
                     /locus_tag="OQQ48_000481"
                     /pseudo
     CDS             29361..31154
                     /gene="hyaB"
                     /locus_tag="OQQ48_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
     gene            31173..31880
                     /gene="hyaC"
                     /locus_tag="OQQ48_000482"
     CDS             31173..31880
                     /gene="hyaC"
                     /locus_tag="OQQ48_000482"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HCS1951364.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMVFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDGTVISTMV
                     NGYRSHKFGKISNKECS"
     gene            31877..32464
                     /locus_tag="OQQ48_000483"
     CDS             31877..32464
                     /locus_tag="OQQ48_000483"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HCS1951365.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLLAAEQSALAQLAAWGSVP
                     QLANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            32461..32859
                     /gene="hyaE"
                     /locus_tag="OQQ48_000484"
     CDS             32461..32859
                     /gene="hyaE"
                     /locus_tag="OQQ48_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HCS1951366.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGVFRFPTTLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            32856..33713
                     /locus_tag="OQQ48_000485"
     CDS             32856..33713
                     /locus_tag="OQQ48_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HCS1951367.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSKSEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            33847..35391
                     /gene="appC"
                     /locus_tag="OQQ48_000486"
     CDS             33847..35391
                     /gene="appC"
                     /locus_tag="OQQ48_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HCS1951368.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWAFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            35403..36539
                     /gene="appB"
                     /locus_tag="OQQ48_000487"
     CDS             35403..36539
                     /gene="appB"
                     /locus_tag="OQQ48_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCS1951369.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLSSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            36552..36644
                     /gene="cbdX"
                     /locus_tag="OQQ48_000488"
     CDS             36552..36644
                     /gene="cbdX"
                     /locus_tag="OQQ48_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HCS1951370.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            36724..38022
                     /gene="appA"
                     /locus_tag="OQQ48_000489"
     CDS             36724..38022
                     /gene="appA"
                     /locus_tag="OQQ48_000489"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HCS1951371.1"
                     /translation="MKAILIPFLSLLIPLTSQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYFGHYQRQRLVADGLLAKKGCPQP
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLKREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(38137..40317)
                     /gene="etk"
                     /locus_tag="OQQ48_000490"
     CDS             complement(38137..40317)
                     /gene="etk"
                     /locus_tag="OQQ48_000490"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HCS1951372.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(40337..40783)
                     /gene="etp"
                     /locus_tag="OQQ48_000491"
                     /gene_synonym="yccY"
     CDS             complement(40337..40783)
                     /gene="etp"
                     /locus_tag="OQQ48_000491"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HCS1951373.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(40771..41910)
                     /locus_tag="OQQ48_000492"
     CDS             complement(40771..41910)
                     /locus_tag="OQQ48_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HCS1951374.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTRNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(41956..44052)
                     /locus_tag="OQQ48_000493"
     CDS             complement(41956..44052)
                     /locus_tag="OQQ48_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCS1951375.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVNDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFEAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            complement(44052..44798)
                     /locus_tag="OQQ48_000494"
     CDS             complement(44052..44798)
                     /locus_tag="OQQ48_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCS1951376.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPMEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVDPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(44795..45439)
                     /gene="gfcB"
                     /locus_tag="OQQ48_000495"
     CDS             complement(44795..45439)
                     /gene="gfcB"
                     /locus_tag="OQQ48_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HCS1951377.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRNV
                     LCNVWQEEVTSTRPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(45546..45851)
                     /locus_tag="OQQ48_000496"
     CDS             complement(45546..45851)
                     /locus_tag="OQQ48_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-rich inner membrane protein GfcA"
                     /protein_id="HCS1951378.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEATNGTEATTGTTGTTTTTTG
                     ATTTATTTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(46293..46505)
                     /gene="cspH"
                     /locus_tag="OQQ48_000497"
     CDS             complement(46293..46505)
                     /gene="cspH"
                     /locus_tag="OQQ48_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HCS1951379.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            46791..47003
                     /gene="cspG"
                     /locus_tag="OQQ48_000498"
     CDS             46791..47003
                     /gene="cspG"
                     /locus_tag="OQQ48_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HCS1951380.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            47014..47202
                     /locus_tag="OQQ48_000499"
     CDS             47014..47202
                     /locus_tag="OQQ48_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCS1951381.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVTLQIRTDHAPLDFTK"
BASE COUNT        11627 a        11731 c        12411 g        11448 t
ORIGIN      
        1 tagcgatgtg cagtcgggta aagtgaatgc cagcgcgaca tttgtacttc attatgacta
       61 atacgtggaa cagattggcg ctcttgattt tcgccgtttt atcgctgctg gtggcgggtg
      121 aattgcgggc aggagtggtg gtcggcggaa cgcgatttat ctttccggca gacagagaat
      181 cgatatctat tttactggct aataccagtc aggaatcctg gctgattaac agtaaaatca
      241 accgcccaac gcgttgggcc ggggggaaag cgtcgacagt gccagcacca ttactggccg
      301 ctccgccact tattctcctg aagcccggta ctacaggcac gttgcgcttg ctgagaacgg
      361 aaagcgacat cttgcctgtg gatcgcgaaa cgctatttga gttaagcatt gccagcgtgc
      421 catccggcaa agttgaaaat cagagcgtaa aagtggcgat gcgctcggta tttaaactgt
      481 tctggcgacc cgaaggtttg ccgggcgacc cgctggaagc ttatcaacaa ttacgctgga
      541 cacggaattc gcagggtgta caactcacta acccaacgcc ttactacatt aacctgattc
      601 aggtgagcgt aaatggtaaa gcgttaagta atgcgggagt tgtgccgcct aaaagccagc
      661 gtcagacaag ctggtgtcag gcaatcgcac cgtgtcatgt cgcctggcgg gcgataaatg
      721 attacgacgg gttgagtgca aagaaggagc aaaatctgcc ctgaaacagg ttcggaaaac
      781 gtttgcgttt tttttgccgc aggtcaattc ccttttggtc cgaactcgca cataatacgt
      841 ccccggtttg cacaccggga atccaggaga gttcatgtac taccccttcg ttcgtaaagc
      901 ccttttccag ctcgatccag agcgcgctca tgagtttact tttcagcaat tacgccgtat
      961 tacaggaacg ccgtttgaag cactggtgcg gcagaaagtg cctgcgaaac ctgttaactg
     1021 catgggcctg acgtttaaaa atccgcttgg tctggcagcc ggtcttgata aagacgggga
     1081 gtgcattgac gcgttaggcg cgatgggatt tggatcgatc gagatcggta ccgtcacgcc
     1141 acgtccacag ccaggtaatg acaagccgcg tctctttcgt ctggtagatg ccgaaggttt
     1201 gatcaaccgt atgggcttta ataatcttgg cgttgataac ctcgtagaga acgtaaaaaa
     1261 ggcccattat gacggcgtcc tgggtattaa catcggcaaa aataaagata cgccagtgga
     1321 gcagggcaaa gatgactatc tgatttgtat ggaaaaaatc tatgcctatg cgggatatat
     1381 cgccatcaat atttcatcgc cgaatacccc aggattacgc acgctgcaat atggtgaagc
     1441 gctggatgat ctcttaaccg cgattaaaaa taagcaaaat gatttgcaag cgatgcacca
     1501 taaatatgtg ccgatcgcag tgaagatcgc gccggatctt tctgaagaag aattgatcca
     1561 ggttgccgat agtttagttc gccataatat tgatggcgtt attgcaacca ataccacact
     1621 cgatcgttct cttgttcagg gaatgaaaaa ttgcgatcaa accggtggct taagtggtcg
     1681 tccgcttcag ttaaaaagca ccgaaattat tcgccgcttg tcactggaat taaacggtcg
     1741 cttaccgatc atcggtgttg gcggcatcga ctcggttatc gctgcgcgtg aaaagattgc
     1801 tgcgggtgcc tcactggtgc aaatttattc tggttttatt tttaaaggtc cgccgctgat
     1861 taaagaaatc gttacccata tctaattatt tcttcgaatt cctctttata agataatcag
     1921 ggctttattt tcagccctgg ttgttttata ttcatcgctg ttgcttattt agacattttg
     1981 tacttttatt gttgaggtta ttaagcgaag cgacaatgga ttgtgttgct gcggttttat
     2041 agggtagggg agaggcagat gcgaattaaa ccagacgata actggcgttg gtattacgat
     2101 gaagagcacg atcgaatgat gctcgattta gccaatggta tgctatttcg ctcacgtttt
     2161 gcgcgcaaga tgttgactcc agatgccttt tctcccgcag gcttttgcgt tgacgacgcc
     2221 gcgctctatt tctcttttga agaaaagtgc cgcgatttta atttatctaa agaacaaaaa
     2281 gccgaactgg tgctgaatgt actggtagca attcgctatc tcaaaccgca aatgccgaaa
     2341 agctggcatt ttgtttccca tggtgaaatg tgggtgccca tgccgggcga tgcggcctgc
     2401 gtttggttga gcgatactca cgagcaggtc aatttgctgg tcgttgaatc tggcgaaaat
     2461 gccgcactat gcctgctggc acaaccctgc gttgttattg cgggtcgcgc gatgcagttg
     2521 ggcgacgcca ttaaaatcat gaacgacagg ctgaaaccgc aggttaatgt tgacagcttc
     2581 agcctcgaac aggcagtcta acgcgccaac ttaagtgcag tcttcggtac acagctacag
     2641 caaagaatgg tgccatcatc gcccattgct gattttttca gcggcgagac ttcgccttct
     2701 aaaatctgaa cacggcaact tccacaaatg cccgcgcggc aagaataagg gatacgaatt
     2761 ccctgatttt ctaattgctc cagcaacacc tgttggttat ttccacgaaa tgcctgtccc
     2821 tgccagtcaa tatctacatt tgcgtccggt tgttgcgtga tgttggcggt atcatcagcg
     2881 gcagctgcgc cgtaaatttt tgccggagcc gttgccagaa tttccacctc atcgccaacg
     2941 cgaatcacgc cgctattacg ggcaattaaa ttctgaccaa aatcgacatc gccgttatcc
     3001 tgggcagtgc ggaaagattg caatgttttt aatggttcgc ctgccggatg tttttgccct
     3061 ttttctgggc tgacggtggt gaaaatacag cggctacaag gtttaaccac atcaaacacc
     3121 acatcaccaa tgcgaatcac tttccagcga tcttcttccc aggctgacgc gccggaaacc
     3181 accagattgg ggcggaattg ctccattttt acactggccg gacaacgttg ttggagatca
     3241 cgtaacgagg cttcgttagc aagaaggtaa ggatagccat cagcaaatga cagaggtaca
     3301 gtgttgtggc gtttcacgcg ccgggtcatt tgtggcccca cccagcgtaa ttgcacttcg
     3361 cgggagaaaa atccacttag ccatttgttg atcgcgtctg gcgcaattcg cgcggtaaaa
     3421 tgtgtgcccc aaacttcggt tggtgcgtct tgtgtggcga aatcagcaaa acgaacatat
     3481 gcgctactgc catctggtgc ggtgagatgc aagccatcat gcacaggtga aggagtaaac
     3541 cgtaccatct ggggaaactg gcgagcggta ataaacgtac cgtcaggttc cgtgatcata
     3601 aagatgcgat cgaaggccag accactgaca tcagccagag catgtgtaag accaatgccg
     3661 cgcatcgatt taacaggatg aataaaaagc cgggttaatg tcgccacggt gcggtcctca
     3721 aatgaaaata agccctcaac tttatgacat gacgcgctta ttagctataa tgcgcaacaa
     3781 ttttcttagc gcctgtccca ttaggctatt ttatttgtca gtttggccct gggttgtgct
     3841 cgaaatcctc acgtacttgt gtacgctccg gtttctccgc gctgtccatg tccagactga
     3901 ctgcaacaat tacgcctact gcggtaggct cttagagtaa aagtgacgat atgaattctc
     3961 tgtttgccag tacggcccgt gggctggaag agctgttaaa aactgaactg gaaaacctgg
     4021 gggccgttga atgccaggtg gttcagggtg gggtccattt caagggcgac acacggcttg
     4081 tttaccagag cctgatgtgg agccgcctgg cctcgcgtat tatgttgccg ctgggcgagt
     4141 gtaaggttta cagcgattta gacctctatc tcggtgttca ggcgatcaac tggacagaga
     4201 tgtttaatcc tggcgcgacc ttcgctgtcc acttcagtgg tttgaatgac accatccgca
     4261 acagtcagta cggcgcgatg aaagtgaaag acgcgatcgt cgatgctttc acgcggaaaa
     4321 atctgccgcg tccaaatgtt gatcgcgatg cgccggatat ccgcgttaac gtctggctgc
     4381 ataaagaaac cgccagtatc gctcttgatc tgagtggtga tggtttacat ctgcgtggct
     4441 atcgcgatcg tgctggtatt gcgccgctca aagaaaccct ggcagccgct attgtgatgc
     4501 gatccggctg gcagcctgga acaccgctgc tcgatccgat gtgtggttcc ggtacgttgc
     4561 tgattgaagc ggcgatgctg gcgaccgatc gcgcacccgg cttgcaccgt gggcgttggg
     4621 gctttagcgg ctgggcgcag catgatgaag ctatctggca ggaagtgaaa gcggaagcgc
     4681 aaactcgcgc ccgtaaaggc ctggctgagt atagctctca tttttacggt tcggacagcg
     4741 acgcacgggt gattcaacgt gcacgcacta acgcccgtct tgcggggatt ggtgaactga
     4801 tcacctttga ggtgaaagat gtcgcgcaac tgaccaatcc gctgccgaaa gggccgtacg
     4861 gcacagtgtt gagcaacccg ccatacgggg aacgtctgga cagcgaaccg gcgctgattg
     4921 cgctgcatag cctgctgggt cggatcatga aaaaccagtt tggtggctgg aatctctctt
     4981 tgtttagtgc ctcgccggat ctgctaagct gcttgcagct gcgtgcagac aaacagtaca
     5041 aggcgaaaaa cggcccgctg gactgcgtac agaaaaatta ccacgttgcc gaaagcactc
     5101 cagacagcaa accggcgatg gtagcggaag actacgccaa ccgcctgcgt aagaacctca
     5161 aaaaattcga gaagtgggct cgccaggaag gaattgaatg ttaccgcctg tatgacgccg
     5221 atctgccgga atataacgtt gccgttgacc gttattccga ctgggtggtg gtgcaggagt
     5281 atgcaccgcc aaaaactatt gatgctcaca aagcgcgtca gcgtctgttc gatattatcg
     5341 ctgcaaccat ttcggtactg gggattgcgc caaacaaact ggtgctgaaa acccgtgaac
     5401 gccagaaggg caaaaaccag taccagaaac tgggcgagaa gggcgagttt cttgaagtta
     5461 ccgaatataa cgctcacttg tgggtgaacc tgacggatta tctcgatact ggtctgttcc
     5521 tcgatcaccg tatcgcccgc cgtatgctcg gtcagatgag caaaggcaaa gatttcctca
     5581 acctgttctc ttataccggt agcgccaccg tgcatgcggg attaggcggc gcgctcagca
     5641 ccaccaccgt ggatatgtcg cgtacttatc tggagtgggc agaacgcaac ctgcgtctga
     5701 atggcctaac cgggcgtgcg catcgcctga ttcaggccga ttgcctggcg tggctgcgtg
     5761 aggcaaatga acagtttgat ctgatcttta tcgatccgcc aaccttctct aactcaaaac
     5821 gaatggaaga tgcgtttgat gttcagcgcg atcatctggc gctgatgaaa gatttgaaac
     5881 gtctgctgcg tgcaggtggg acgatcatgt tctcgaacaa caaacgtggc ttccgtatgg
     5941 atctcgacgg cctggcaaaa ctgggactga aagcacaaga aattacgcaa aaaacgctct
     6001 cccaggattt cgcccgtaac cgccagatcc acaactgctg gctgattacc gcagcctgaa
     6061 aggaatagta atgtcattaa tcagtatgca tggcgcatgg ctgtcgttca gcgacgcgcc
     6121 gcttctcgat aacgcagaac tgcatatcga agataacgaa cgtgtttgtc tggtgggccg
     6181 caacggcgcg ggcaaatcga cgttaatgaa aatcctcaac cgtgaacaag ggctggatga
     6241 cggtcgcatt atttacgagc aagatttgat tgtagctcgt ctgcaacagg atccgccgcg
     6301 taacgttgag ggtagcgttt atgatttcgt tgccgaaggc attgaagaac aagcggaata
     6361 tctgaaacgc tatcacgata tttcgcgcct ggtgatgaac gacccgagcg agaaaaatct
     6421 caacgaactg gcgaaggttc aggaacagtt ggatcaccac aacctgtggc agctggaaaa
     6481 ccgcatcaac gaagtgctgg cgcaactggg gttagatcct aatgttgcgc tgtcgtcgct
     6541 ttccggcggc tggttgcgta aagcggcatt aggacgcgcg ctggtgagta atccgcgcgt
     6601 gctgttgctt gatgaaccga caaaccacct ggatattgaa accatcgact ggctggaagg
     6661 gttccttaaa accttcaatg ggacgattat tttcatctcc cacgaccgtt cgtttatccg
     6721 caatatggcg acgcgcattg ttgatctcga tcgcggcaag ctggtgacct atccagggaa
     6781 ttacgaccag tacctgcttg aaaaagaaga agccctgcgc gtggaagaac tacaaaatgc
     6841 cgagttcgat cgcaaactgg cgcaggaaga ggtgtggatc cgccagggga tcaaagcacg
     6901 ccgtacccgt aatgaaggcc gcgtacgcgc cctgaaagca atgcgtcgcg agcgtggtga
     6961 acgtcgcgaa gtgatgggta ccgcaaagat gcaggtggaa gaggccagcc gctccggtaa
     7021 gatcgttttc gaaatggaag acgtttgcta ccaggttgac ggtaagcaac tggtgaaaga
     7081 tttttcggcc caggttctac gtggcgacaa aattgtcctg attggtccga atgggtgcgg
     7141 caaaaccacg ctgctgaaac tgatgctcgg tcagcttcaa gcggacagcg ggcgtattca
     7201 cgttggcacc aaactggaag tggcttattt cgatcagcac cgcgcggaac tggatcccga
     7261 taaaacggtg atggataaca ttgccgaagg taagcaagag gtgatggtta acggcaagcc
     7321 acgccacgta ttgagctatt tgcaggactt tctgttccat ccgaaacggg cgatgacgcc
     7381 ggtacgtgcg ctttctggcg gtgagcggaa ccgcttgctg ctggcgcgtt tgttcctcaa
     7441 accaagcaac ttattgattc ttgacgaacc gaccaacgat cttgatgtcg aaacgctgga
     7501 actgctggaa gaactgatcg acagctatca gggcacggta ttgctggtta gccacgatcg
     7561 tcagtttgtc gataacaccg ttacagaatg ttggatcttc gaaggcggcg gtaaaattgg
     7621 tcgttatgtc ggcggttatc atgatgcccg tggtcagcaa gagcagtatg tggcgctcaa
     7681 acagcctgcg gtgaaaaaaa ccgaagaagc cgccgcggca aaagcagaaa ctgtaaaacg
     7741 cagcagtagc aaactaagct ataaattgca gcgcgaactg gagcagctac cgcaattgct
     7801 cgaagatctg gaggcgaagc tggaagccct acagacgcag gtggcggatg cttccttctt
     7861 cagtcagcca catgagcaga cacaaaaagt gctcgccgat atggctgctg cagagcagga
     7921 gctggagcaa gcctttgaac gctgggagta tcttgaagcg ttaaaaaatg gtggctgatc
     7981 gcaaaatagt cgatttaaac agagacagct gacaacggta aaattgcctg atgaactggg
     8041 tttatcaggc atgaaaacca acactttgtt agtcatctgt aaaatagcgc atcattaagg
     8101 agtaccaatg tgcgaacatc atcatgccgc gaagcacatc ctgtgctcgc agtgtgacat
     8161 gctggtggcg ttaccgcgcc ttgagcatgg tcagaaagcg gcatgtcccc ggtgtggcac
     8221 aacgttaacc gtggcgtggg atgcccctcg gcagcgtccg accgcctatg cgttggctgc
     8281 actgttcatg ctgttgctgt ccaacttgtt tccttttgtg aatatgaacg ttgcaggagt
     8341 taccagtgaa attacattac tggaaattcc cggcgtgctt ttttctgagg actacgccag
     8401 cctcggcacc tttttcctgt tgtttgtgca actggttccc gcgttttgtc tgataaccat
     8461 tctgttactg gtgaatcgcg cggaattacc ggtccgttta aaagagcaac tggcacgggt
     8521 gctttttcaa ctcaaaacct ggggaatggc ggagattttc ctcgcgggtg tgctggtcag
     8581 tttcgttaaa ctgatggctt acggcagcat tggggtaggc agcagctttc tcccctggtg
     8641 tttattttgt gtcctgcaac tgcgcgcttt tcagtgcgtt gatcgtcgct ggttatggga
     8701 cgacatcgcc ccgatgccag aactgcgcca gccgctaaaa ccaggcgtca cggggatacg
     8761 tcaggggctg cgttcgtgct cctgttgtac ggcaatcctt cctgctgatg aacccgtgtg
     8821 cccgcgttgt agtaccaaag ggtacgttcg gcgtagaaac agcctgcagt ggacactcgc
     8881 gctgcttgta acgtccatca tgctgtatct tccggctaat attttgccca tcatggtgac
     8941 ggatttatta ggctcgaaga tgccgtcgac gattctcgct ggggtcattc tgttatggag
     9001 cgaaggctct tatcccgtcg ctgcggtgat ctttctggcc agtattatgg tgccaacgtt
     9061 aaagatgatc gccatcgcgt ggctgtgttg ggatgccaaa gggcatggca agcgcgacag
     9121 tgaaagaatg catttgattt atgaagttgt tgagtttgta ggccgctggt cgatgattga
     9181 cgttttcgtt atcgcggtgc tctcggcgct ggtgcgtatg ggaggtttaa tgagtattta
     9241 tccggcaatg ggtgcattaa tgtttgcttt agtcgtcata atgacaatgt tttctgctat
     9301 gacgtttgac ccgcgtttgt cgtgggatcg tcaacctgaa tcagagcatg aggagtcctg
     9361 acagtatgga atctaataat ggggaagcca aaatccagaa agtgaagaac tggtctcccg
     9421 tgtggatatt tcctatcgtc acggcgctca ttggggcctg ggttctattt tatcattaca
     9481 gccatcaggg accggaagtg accctgatca ccgcgaatgc ggaaggaatt gaaggtggca
     9541 aaaccaccat taaaagccgt agcgttgacg tcggcgtggt tgaaagcgcc acactggctg
     9601 atgatttgac gcacgttgaa atcaaagcgc ggctgaattc cggtatggaa aaattgctgc
     9661 ataaagacac cgtcttttgg gtggtgaaac cgcagattgg tcgcgaaggg attagcggcc
     9721 tgggaacgct gctgtctgga gtttatatcg aactgcagcc aggcgcgaaa ggcagcaaaa
     9781 tggataaata cgatttgctg gactcgccac cgttggcccc gcctgatgcg aaaggtatcc
     9841 gtgtgattct cgatagcaaa aaagccgggc agctctcgcc aggagatccg gtgctgttcc
     9901 gtggctatcg ggtaggttcg gttgaaacca gcaccttcga tacgcaaaaa cgcaatatca
     9961 gttatcaact gttcatcaat gcaccttatg accgactggt gaccagcaat gttcgcttct
    10021 ggaaagatag tggcattgcg gttgatctga cgtcagcggg gatgcgtgtg gagatgggct
    10081 cattgacaac gctgcttagt ggcggtgtta gctttgatgt gccggaaggt ctggatttag
    10141 ggcagccagt ggcaccgaaa acagctttcg ttttgtatga tgatcagaag agcattcagg
    10201 attcgttgta caccgatcac attgattatc tgatgttctt taaagattcg gtacgcggtc
    10261 tgcaaccggg agctccggta gaattccggg gtattcgcct gggtaccgta agcaaagtgc
    10321 cattctttgc gccgaatatg cgtcagacat ttaacgatga ttaccgtatt ccggtactga
    10381 ttcgtatcga gccagagcgg ctgaaaatgc agcttggcga aaatacggat gttgttgagc
    10441 accttggcga attgttgaaa cgtggtttac gcggatcgct gaaaaccgga aacctggtca
    10501 ctggtgcact gtatgttgat ctcgatttct atccaaatac gcctgcaata accggtattc
    10561 gtgaatttaa tggttatcag attatcccga ctgttagcgg cggcctggcg caaatccagc
    10621 aacgactgat ggaagcgttg gataagatca acaaactgcc attgaatccg atgattgaac
    10681 aggcaaccag tacgctttct gaaagtcagc gcacaatgaa aaacctgcaa acgacgctgg
    10741 atagcatgaa caagatcctc gctagccagt cgatgcagca gttgccgacg gatatgcagt
    10801 caacgttgcg tgaattgaat cgcagcatgc agggcttcca gcctggctcc gcagcctaca
    10861 acaagatggt ggcggatatg cagcgccttg atcaggtgtt gcgagaactg caaccggtgc
    10921 tgaaaacgct caatgagaag agtaacgcgc tggtatttga agcgaaggac aaaaaagatc
    10981 cagagccgaa gagggcgaaa caatgaaaaa gtggctagtg acgattgcag cactgtggct
    11041 ggccggatgc agctccggcg aaattaataa aaactattac cagttacctg tggtgcagag
    11101 cggtacacaa agtaccgcca gccagggcaa tcgtctgtta tgggtagagc aggtcactgt
    11161 tcctgactat ctggcgggga atggtgtggt ttatcaaacc agtgatgtga agtatgtgat
    11221 tgccaacaac aacttgtggg ccagcccgtt ggatcaacag ttgcgcaaca ccctggttgc
    11281 caacctgagt acgcaactgc ccggctgggt ggttgcctcc cagcctctgg gaagcgtcca
    11341 ggacacgctc aatgttaccg taacggagtt taacggtcgc tatgatggca aggtcattgt
    11401 cagtggtgag tggctgttga accaccaggg acaactgatc aaacgtccgt tccgtctgga
    11461 aggagtgcaa actcaggatg gttacgatga gatggttaaa gtgctggccg gtgtctggag
    11521 tcaggaagcc gcttctattg cacaagagat aaagcgtcta ccttaattat aaagatttgt
    11581 aagtataacc gtctccggta tgttgcctga ggcggttttt ttgtttctaa cgtgcggaaa
    11641 aatttgttcc tcttcacatt ttttgtacaa ccgacatgcc cgtgtagctc acaaatatga
    11701 cagtggcgtg aattttgcgc attgacggca gttatgattc gcggtattgc ttaactgtga
    11761 ttgcacattt agtaatcact gttttctttt ccaccagaaa ccagtatgag ggaaacgagg
    11821 catgaagaga caaaaacgag atcgcctgga acgggcacat caacgtggtt atcaggccgg
    11881 catcgccgga cgctcaaaag aaatgtgtcc ctatcagacg ctgaatcaaa ggtcacaatg
    11941 gctgggaggc tggcgagaag ccatggcgga cagggtagta atggcctgat tctgtctctt
    12001 taaaaagaaa cctccgcatt gcggaggttt cgccttttga tactctgtct gatgataatc
    12061 agaaggcaga cgtatcctgg aacagaccga ctttcaggtc gctggcggta tagatcagac
    12121 gaccatcaac cagcacttcg ccatctgcca gacccataat cagacgacga ttaacaatgc
    12181 gtttaaagtg aatacggtag gtcacttttt tcgctgtcgg cagtacctga ccagtgaatt
    12241 tcacttcgcc aacgcccagc gcgcggcctt taccttcgcc gcccagccag ccgaggtaga
    12301 accctactag ctgccacatt gcgtccaggc ccaggcatcc cggcataacc ggatcgccaa
    12361 tgaagtggca tccgaagaac cacagatccg gattgatatc cagttctgct tcaacatacc
    12421 ctttgtcgaa gttaccaccc gtttcggtca ttttgaccac acggtccatc atcagcatgt
    12481 tcggtgctgg caattgcggg cctttagcgc caaacagttc accgcgacca gaggcaagaa
    12541 ggtcttcttt tgtataggat tcgcgtttat ctaccatgtt ctctgtaagc cttattttat
    12601 tgaagcacgc aggatagcta acacgtgtac gctgaacaag tccgatcagt tcggaataaa
    12661 ccagttcagc caacgtaatg gccatggaaa acggtgacgt ccttcctgtt gcgatgcttg
    12721 cgcgatacgt tcctggatgg tttgcatcag cgtcgtttgg ccttcgccat cccacaccag
    12781 atttaataat aacggcagtg cgtcagtcac atcgtctact gcccagatgg tgaatttgcc
    12841 ttcttctacc gctttcacca gttcactgtg aagacttaaa tggcgaacgt tagctgtggg
    12901 gataatgaca ccttgtttcc cggttaactc acgttgctgg caaatagcaa agaagccttc
    12961 gattttctca tttaaaccac cgaccggctg ggcgcgaccg aactgatcga ctgaacctgt
    13021 gatagcgata ctctgattca ccggcacatc ggcgagggcg cttatcaggg cgcagagttc
    13081 agccatcgag gcactatctc catcaacttc actgtatgac tgctcaaatg tcagcgatgc
    13141 tgagaagggg atctgttgct caagctgtag ttccgacatc aggaacgctt gcatgatcat
    13201 catccctttc gcatggatat tgccgccaag ttccgctttg cgttcgatgt cggtgaattc
    13261 accatcgcca atatgcacaa cgcagctaat gcgagaaggt tcgccaaaag cgcgtggatg
    13321 acccggaaat tcaatgaccg aaagggcgtt aatttgcccg atgcgttcgc cttcggtttc
    13381 aatcaggatt tgctcctgaa ggatctcatc ctgcatacgt tcagcgagga aaccttcgcg
    13441 ccattcacgc tgctgcagca ttaagtttag ctgctcgccg gagaaggtgt cgccatcaca
    13501 cagggaggcg acctctttac actggcggag gatccactgc gggctaagcg gaagtgtttc
    13561 ttgttcaccg gtgtagcgtg ctgcttcgcg gataagtacc ggccaggcat ccgctcccgg
    13621 ggcaggtaag tgattatgtc tggcggtaaa tgtcacccag cgacaccact ggcttactga
    13681 ctccgcatcg acaatctgca gagtatcttc aaattcgcta taaatagcct gctctgaaag
    13741 ctctggctcc atctcctgga aatcagccaa tgattcgcgt tcgcctacca gaatgacttt
    13801 cagcttcaat ggcatcgaag gcacagagac ggggagaggg cgcgactcat caaacgcaac
    13861 ccagtcaaaa cgctcgcggt taacgatatt tttcagccgc atccacagca gaggttgcgc
    13921 cagcagtgta cgcaaagaga tgatgagaat accgccattt gcctgatgca ccagaccagg
    13981 ctgcagggta atgtcgccat taaactggcg caggcagcca aagagttgct ccgcttctac
    14041 ccagtcggca gcgacaactt gcgttaaagt cgcaaaatta tcatctgcac tcactgcgtg
    14101 acgtaagcgg atggagtggc cggaaacctc atagtgatcg cccaccagtt ggcctgcatc
    14161 gctttgtagc gtacgcgcgg catcggcaat aagattcaga tactcagact cttccggggc
    14221 cttagccagc ataaaagagg aggatgctcg cgtatgcagc aactgctcca gcgcaaattg
    14281 cagccgcggt tgagtatcac tgaataaagg atcgttttcg tcaatcaaat gtggctgagc
    14341 aaatatttcc tgatagctat cggtatcagg aaccaggtca cgccatgcaa gtttcgtaat
    14401 ggtcaaagtt gatgtttttt agtctgttgt caaagccgcg attataccgt aaccggcact
    14461 acagcacacg tagaaagcac cgacaatact cctggcatgg gcgttaaagc tcacaggatg
    14521 gagattcttt tcttcactgg cctaaaaagc tgatattctg taaagagtta cacggtaaca
    14581 ttgagatcgc tatgaaatat caacaacttg aaaatcttga aagcggttgg aaatggaagt
    14641 atctggtgaa gaagcatcgc gaaggggagt taattacccg ttacatagaa gccagtgccg
    14701 cccaggaagc cgtcgatgtg ttgctctcgc tggaaaatga acccgtgctg gtaaatggct
    14761 ggattgacaa gcatatgaat ccggaactgg tcaatcgcat gaagcaaacc attcgggcca
    14821 gacggaagcg ccattttaat gcggaacacc agcatacgcg caaaaagtcg atcgatctgg
    14881 aatttattgt ctggcaacgt ctggctggtc ttgcgcagcg tcgcggtaaa acgctttctg
    14941 aaacgattgt tcagctgatt gaagatgcgg aaaacaaaga gaaatacgcg aataaaatgt
    15001 cttctctgaa gctggatctg caggcattgc tgggtaagga ataactgacg aaagtcagtt
    15061 caatttacta aaggcaaaaa aaaccccgca gcagcggggt ttttctacca gacgataact
    15121 taagcctgcg gctgagttac aacgtctttg atacctttaa cttcgatctc tacgcgacga
    15181 tccggagcca ggcagtcgat cagtgcagca cgctgtttca cgttgtcaca ggtgttgcca
    15241 gtaaccgggt tggattcgcc cataccacgt gcggagatct tgtctgccgg gatacctttg
    15301 gagatcaggt aatcaacaac agactgagca cggcgctcgg acagaccctg gttgtaagcg
    15361 tcagaaccga tgcggtcggt gtaacccaga acaactacgg aaccgtcttt cggatccagg
    15421 ttgctcagct ggctgtacag ctgatccaga gcagcctgac cttccggttt cagggttgct
    15481 ttgttgaagt tgaacagaac gtcagacttc agagtgaagt gcttggtctg tacttccggt
    15541 gccggagctg gagccggagc aactactgga gctgcttcgc cctgaccgaa acggtaggaa
    15601 acacccaagc tcagcatgcc gttgtccgga cgagtgccga tggtgtgtgc gtcaccgatg
    15661 ttgttggtcc actggtattc cagacgggta gcgatttcag gagtgatcgc gtactcaaca
    15721 ccgccagcga agaccggaga aacgccggtg tcgtggtttt taccataaac gttggattta
    15781 gtgtctgcac gccataccat accacccaga cgagtgtaga tgtccaggtc gtcagtgatt
    15841 gggtaaccca gtttagcggt cagttgaacg ccctgagctt tgtatgcacc gttttcaacg
    15901 ctgcctttgt acggcatacg acctaaccag tcgtaaccca tttcaaagcc aacatacggg
    15961 ttaacctggt aaccaccaaa agcaccagcg cccagttggt tttcatgggt cgggccattg
    16021 ttgttgatga aaccagtgtc atggtactgg gaccagccca gtttagcacc agtgtaccag
    16081 gtgttatctt tcggagcggc ctgcgctacg gtagcgaaac cagccagtgc cactgcaatc
    16141 gcgatagctg tctttttcat tttttgcgcc tcgttatcat ccaaaatacg ccatgaatat
    16201 ctccaacgag ataacacggt taaatccttc accgggggat ctgctcaata ttaactctac
    16261 cgatatcttc ggcttatgcc gagcacccct ggcgatgtaa agtctacaac gtagttgaaa
    16321 acttacaagt gtgaactccg tcaggcatat gaaaaaaaag tcttgtataa ggtatgttta
    16381 atcttttttg tcagcgacaa tttacagaag aaaatcgcgg aaaccgcttc agacaagcct
    16441 ccgcaaggaa aattagtcac gactgaaagc attggctggg cgacaaaaaa agttccagga
    16501 ttaatcctaa atttacttaa tgatacaaat tagagtgaat ttttagcccg gaaagttgtc
    16561 tcgtggcgtg agaggatgcg cttaccggac gcataataaa ccccatagcg ttaccttcat
    16621 ttgccgcatc aacaagttca gcatgctctt cttcagtcaa atcatctgcc aaccaaccga
    16681 tcaccacact gtaattgccc gtgcgtaaag cgcgaaccat tgactccaca gtgtggcaag
    16741 gggagagctg gctaatctgc attactttcg ttaagggtag cccagatgcc tgaacccatt
    16801 cccgactcag tttttgttgc ggtgttaacc agagttgcca gcgcgattgc tgaccgagtt
    16861 gctgtaacaa tggcaacagt agaagttgcg tcatcatggg ctgatcttcg cgatagacaa
    16921 cttcactgat aagcccggct gtagtgtttt ccgtagagac acgcgcaatt ttacttgctg
    16981 cggatgagaa cgacgaagaa cgatgtgcat agcctgaagt gtacataatc aatccagccc
    17041 ctgtgagtta ctgtatgaat gtacagtaca tccagtgaca acaaagatca accctatttt
    17101 cggaaagagc ctcgcaaatt ttttcgttgg tgacgggaaa acataaatta atcttgcccc
    17161 ttaagaataa gttgcctatt ttcgtagtta acggatccgt taatgtgaat cattctttta
    17221 tgttatgatt ttaaaaggaa ttttatgaaa agcctctcct ataagcggat ctataaatca
    17281 caagaatacc tggcaatgtt gggcacaatt gaataccgat cattgtttgg cagttacagc
    17341 ctgaccgttg acgacacggt gtttgcgatg gtttctgatg gtgagttgta tcttcgggct
    17401 tgtgagcaaa gtgcacagta ctgtgtaaaa catccgcctg tctggctgac atataaaaag
    17461 tgtggccgat ccgttaccct taattactat cgggttgatg aaagtctatg gcgaaatcaa
    17521 ctgaagctgg tgcgtctgtc gaaatattct ctcgatgcag cgctgaaaga gaaaagcacg
    17581 cgcaataccc gggaaagact gaaagatttg cccaatatgt cttttcatct ggaagcgatt
    17641 ctcggggagg tggggattaa ggatgtacgg gcgttacgta tacttggggc aaaaatgtgt
    17701 tggttgcgac tgcggcagca aaacagtctg gtgacagaaa agattctgtt tatgcttgaa
    17761 ggtgccatta tcggcattca tgaagctgcg ctcccggtgg cacgccgcca ggagcttgca
    17821 gaatgggctg actcacttac gccgaaacag gagtttcctg cggaacttga gtaatctggc
    17881 gttgcagacg accaatctca ggcagtaatg caatcaataa tccaacttgt tgtacgacca
    17941 gaggttcttt gctgtctgcc cgtggttcaa gttgctgcat ccgctgtttc aggctagcta
    18001 atgcctcatt gacgcgttct tcatcagcag gttgatgatg taacgcgtca tcaacatagc
    18061 aaactgcgtc atcaagaaac gccagaattt caggattagt taactgctcc cggtgagcac
    18121 cgagggctga gatatagctg gtaaacgtat ggttaaggca cagcaaccga aacgcggctt
    18181 cgcgaatttg cggggtaacg ttcggctcgc tggacatatt tgataccacc gacgccagct
    18241 cagcatcacg gttgtgtgca tcgcggcggg caatacgata cgccagacgg ttatcacgcc
    18301 cctgatggta ttgctccagt atggcatcga gataccgaca gttggcctct gtggcgcgtt
    18361 cgagcatgcg cggcaaattg cgaaactgcc agtcaggcca gatgtagctc actgccgccc
    18421 acgcaatggc acaaccaatc agcgtatcga ttacgcgagg taacgctact tcaaaacctt
    18481 cacccagtaa gttaaaacac agtagcacca aaagtgtgat gaacatcgtt gcatgagcgt
    18541 attgcacgtt acggaaggca aaaaagagca cgccggtaat aaccagcagc accagctgcc
    18601 cttccagtga tggcacaaac cacagcacag gaatgccaat ggcgatacct accagcgtac
    18661 caataatcct taacttcagg cggtggcgcg tggcgttata gtttggctgg cagacaaaca
    18721 aacttgtcag caagatccaa aacccgtgat gcattccggt tatttgaatg atggcgtagc
    18781 cgaagcacaa caccagcgac attcttaccg catgacggaa gagggcggat tccggcgtga
    18841 agtgacggct aagacgcagc cagatatcac tcaacccgtg cgggctgtca tcagcgagct
    18901 cattttcgtc attattatgg ggtagtgcct gggcctgttc tgattcaatt gttgccagtt
    18961 gggcatcaat ggcgcgtaaa ttgttcagca aaaatcccag tgttttgagt aaatcggcgg
    19021 gtgcgccgtt atcgcgcatc cgctccagcg cagcatcaat atgcgtaaaa gcgcgctcaa
    19081 aatgcggatc atgttgataa ggctgacgca acaaaataca gcgtgacagt tgctggcacg
    19141 cctggccctg catcgacatc agccgctgaa aacggaacag cacgtcgctg tggcgaaaat
    19201 gttcacgcaa tgtttgatac tgaatatgag aagagctggc acgctcgtga atatcctgtg
    19261 cgacaaagta ataatgcagc gtgcgacgcg ttccccgttg accacgatcg ccacgtaagc
    19321 gggtcagcag cgagagtttc gtctgattca atgtcgccat cagcagaccg ttggcgagag
    19381 ccaaatcgta cagcggtgcc tggctttcat cttcaatatc aggatcaaac atgcgcgact
    19441 tgagctcaag ataacgcgcc agttgttcat agcaacgcgc caggttgtcc tgcagcgggc
    19501 ggaccgggaa cagcagatga ccaataagtg tcaggacgtt gtaccagacg gcaccggcca
    19561 gcagatacat cggctgctga taccagtgct catacagtga tgttcccaac atagtgtaaa
    19621 tggcgatcag caatgcaccg aaggcaattg ttgcatagcg ttgacccaga ccgccgagca
    19681 aaatgaagcc gctggtagag agcgttaagc caatcgcaaa tagccagggc cagggaaaca
    19741 gcaattctac tgaggccgag gcgataaaaa agcagaacag cgtaatgatg aggttacgca
    19801 aacgtcccgc cagtcggtca tcgagatcgg tcagcgccgc tgccaccatc cccagcgtta
    19861 gcggaatcgt cagttttaca tcacccagcc accacggaaa cgctgtggtt ccacaaagcg
    19921 caataaaaat acgcgcgtaa tacagccagg cgctgttcca ggtatagcgt ttgagcaaag
    19981 gacttagcat aaaggccata ttagtggatt attcaaaacg acgacgtgca ttggcttcac
    20041 gcgcagcttg cgctgtttct accgatacca cgcgacgacc aaccggccat agtgcaatag
    20101 cggcaatttt aaagttcgca atgccgacag gaatgccaat gattgaaata cattgtgcga
    20161 tgcccgttgc aatgtgcatc aggcataacc accagccaaa gaaaatcagc cagaaaatat
    20221 tcaataccgt accgccagta ttcagcagca catttttgcc agccgggttc agttcatcga
    20281 catgaatagc ttcattgcca taaggcacca gagacagttt agtgatctcc cagcaggatc
    20341 gtgtcagcgg taaggtaaaa atcagcacaa tactgaccag agtcgccaac agccagccca
    20401 gagtggtggc aaatccgcca agcacaaagt tcagaatgtt caaaacggta cgcataaaac
    20461 ctcgctttac tgtggttttc agtaatgggc ggcaattgta acgttttttt gggctggagc
    20521 acgttttctc tgacggttac actgataaga aataatttcg tgtggatcta cagagtcatg
    20581 gaactgaaag cgacaacgct tggaaaacgt ctggcacagc acccttacga tcgggcggtg
    20641 atcctcaatg ccgggattaa agtctccggc gatcgccacg aataccttat tcctttcaat
    20701 caattactgg cgattcactg taagcgtggt ctggtatggg gcgagctgga atttgtactg
    20761 ccggacgaaa aagtggtgcg tctgcacggc accgaatggg gcgagacgca gcgtttttac
    20821 catcatcttg atgctcactg gcggcggtgg agtggcgaga tgagcgaaat tgcgtctggt
    20881 gttttacgcc agcaactgga tttgattgcc acgcgcactg gagaaaataa atggctgacg
    20941 cgtgagcaaa cctctggcgt tcagcaacaa atccgccagg ctttgtcggc gttgccgttg
    21001 ccggttaacc gactggaaga attcgataac tgccgtgagg cgtggcgtaa atgtcaggcc
    21061 tggttgaaag atattgaaag cgctcggttg cagcataacc aggcgtatac cgaagccatg
    21121 cttaccgagt atgcggattt tttccgccag gtcgagtctt caccgctgaa tccggcgcag
    21181 gcccgggcag tcgttaatgg cgagcattct ctgttagtgc tggcaggtgc aggaagcgga
    21241 aaaacgtcgg tgctggtggc ccgtgcaggc tggttgctgg cacgtggtga agcgtcccct
    21301 gagcaaattt tattgctggc gtttggtcgc aaagccgctg aagagatgga cgagcggatt
    21361 cgcgaacggc tacataccga agacattacc gcacgcacgt ttcatgcgct ggcgctgcat
    21421 attattcagc agggcagcaa aaaagttccg atagtcagca aactggaaaa tgataccgct
    21481 gcccgtcatg aactctttat tgctgagtgg cgcaagcaat gcagcgaaaa gaaagcgcag
    21541 gcgaagggct ggcggcaatg gctgacggaa gaaatgcagt ggtcagtgcc agaaggtaac
    21601 ttctgggatg atgaaaaatt acagcgtcgc cttgcctctc gcctcgatcg ctgggtaagt
    21661 ctgatgcgga tgcacggtgg tgcacaggca gaaatgattg ccagtgcacc cgaagagatt
    21721 cgcgatctgt tcagtaaacg tatcaagttg atggccccgt tattaaaagc ctggaaaggt
    21781 gcgctgaagg cagaaaacgc tgtcgatttt tcgggcctta ttcatcaggc gattgtgatt
    21841 ctggagaaag gtcgctttat cagcccgtgg aagcatattc tggttgatga atttcaggat
    21901 atctcgccgc agcgggcagc gttgttagcg gcattacgca agcaaaacag tcagacgacg
    21961 ttgttcgctg ttggtgatga ctggcaggcg atttaccgat tcagcggtgc gcaaatgtcg
    22021 ctcaccaccg ctttccatga aaactttggt gaaggcgaac gttgtgattt agacacgact
    22081 taccgtttta acagtcgtat cggtaaggtg gcaaaccgtt ttattcagca gaacccaggc
    22141 cagctgaaaa agccgctaaa cagcttaacc aatggagaca aaaaagccgt cacgttattg
    22201 gatgagagtc aacttgacgc tttgctggat aagctctctg gttatgccaa accggaagag
    22261 cgcattctga tcctggcgcg ttaccatcac atgaggcctg ccagcctgga aaaagcggca
    22321 acacgctggc cgaagttgca aatcgacttt atgaccattc atgccagcaa agggcaacag
    22381 gcggattacg tcatcatcgt tggcttgcag gagggaagtg atggttttcc ggctgcggcg
    22441 cgggagtcga ttatggaaga ggcgctactg ccaccggttg aggatttccc ggacgctgaa
    22501 gaacggcggt taatgtacgt ggcgctgacc cgggcacgcc atcgggtatg ggcactgttt
    22561 aacaaagaga atccctctcc ctttgttgaa atactgaaaa atctggatgt gccggtggcg
    22621 agaaaaccgt aagaaacagg tggcgtttgc cacctgtgca atattacttc agacggtccg
    22681 cgagataacg ctgataatcg gggatcagaa tatcgaccgc gtcgttgaaa tgcggcgact
    22741 ggattatgaa gtctgccgtt gccacgttgg tggcgaccgg aatgttccat accgtcgcca
    22801 gacgcagcaa ggctttcacg tcaggatcgt gcggcacggc atttagtgga tcccagaaga
    22861 aaatcaatac atcaattttc ccttctgaga tcaatgcgcc aacctgctgg tcacccccca
    22921 ttgggccact caacatcgcg ttgacgttca tgccggtcgc gcgggaaatt aagttaccgg
    22981 tagtgcctgt tgcatacagt acgtgttgtt ccagtaacgg ttgatgccgt tccacccagc
    23041 tcatcagcat ttgtttgcag tgatcgtgtg ccaccagcgc aatatgtttc cgcgcaggta
    23101 aagtgcgagt cgtcagttcc ataatgtaca tccgtagtca actttcctac agattactgt
    23161 aagcacttat cgctgcaaga taaagaccga aaaagcctgc gcacaggcac aaaaatctca
    23221 ggaagatggt tgtttttccg cccactgcag gaaagtattt cgcgtttgtg ggtcagccag
    23281 tttaaaccaa tacttcagcc gttgttctgt gagcacctga gactgcggtg gaatagcatc
    23341 cagctcggaa acacccgaaa gcagcgtact gtcatctgcc atcatcgtgg caaattgcgg
    23401 tagtgaagcg cgtttcgcgg atttgttata gcgttcaacc tcgacctcat aatcaatcgt
    23461 ttttgccgtt gaggtaatgg cgagaatatc cagctttacc ggaatcggcg tcgcatcgcc
    23521 atccaacaat tcaaggcgcg gcgcggcatc aaaatggtta gcttcccgct cattctccag
    23581 gcgaggcagg cgaaaattga cctggttgat gagctgggta ttaaaactga ccactagcgg
    23641 tggggagata tacagccgtt cttcactatt ggaaagatga atcgtcttct caacgcgaaa
    23701 cactaactga tgcggtccat tatccagttc aatgctgtcc gcaccgcgca gcagagagct
    23761 ggaaaccttc ttgccgtcga gtaccagaag atcgacatcg gttgaaagcc gcagcgtggt
    23821 ggcaaaaacg gataccggca aacataatgc aatcaaggtg gtcacgatgc cggttttcat
    23881 aggtagctcc tgtcaaaaga ccgcccgaat aataatgtgt caaaattttt ctttaatcat
    23941 acttactaac atatagacat atttcccgct tttgctctca ttcattcgta ttagctgcat
    24001 ggttggcatg tcggctttgt cgtacacttt gttaaacagt ctggaggaaa taatgaaaga
    24061 aaccgatatt gcaggcattt taacgtccac tcacaccatt gcgctggtgg gggcaagcga
    24121 caaacccgat cgtcccagct atcgcgtgat gaaatatcta ctcgaccagg ggtatcatgt
    24181 cattccggtt tcgccaaaag ttgccggcaa aacgctgctg ggccagcagg ggtatggcac
    24241 gctggcagac gttccggaaa aggtcgatat ggtcgatgtt ttccgtaatt cagaagcggc
    24301 gtggggcgtg gcacaggaag ctattgccat tggcgcgaaa accctctgga tgcaactggg
    24361 ggtgattaat gagcaagcgg cagtactggc acgggatgcc gggttgaatg tggtgatgga
    24421 tcgctgcccg gctattgaga tccctcgcct gggcctggcc aaataaaaaa tccccggaag
    24481 gcaaaaacct tccggggatt cgttcaggga tcagttacgc agacgcgggg cctggagttg
    24541 tttgcggatg gtctgcgcca gctcatccat ggatggctgt tcaggatgtt catcctgcaa
    24601 ttcgctactt agctgggctt cagccaggta ggtatgaacc ggtaggccgt tatcgtcctc
    24661 catcactacg tgataccacg gagcggcgcg aagctcgtca tttaccgcca gctcatcagg
    24721 cgacggttca gagagcgaat aaaccgggtc gatatccacg accactccga gataacctaa
    24781 cagggaatgg cggacctgct ggccgatacc gaatttgctg gcaatcatag tcacctcccg
    24841 ggaaatcttt acctttgata taagggtaaa attccacatt tcaagttaca tgacgcgaca
    24901 ggcaaaccct ttcagatata gcccttccgg ataggtagcg atcaccggat gatcggctgc
    24961 ctgacggaac tgctctataa attgtacatc acggccggca tcaattgcgg catccgcgat
    25021 gattttctga aataaatcgc tggtcatcag accggaacag gagaaagtca ggagaatacc
    25081 gccttcattc agcaactgaa tcgccagcat gttgatatct ttatagccac gacacgcgcc
    25141 catcaactgg cttttattct caacaaactt cggcgggtcc atcacgataa cgtcaaattt
    25201 ttcaccgcga tcgcgataag tacgcagcaa tttaaagaca tcatcacgga caaactcagc
    25261 cttgctcaga tccagtttgt tcagctcaac gttctgccgt gcaatatcca gcgcttcctg
    25321 ggaggtatca acgctgacaa cctggctgca accgcccatc agtgccgata cggcgaaacc
    25381 accggtatag gagaaacagt tcagcacacg tttattttca acgtagcggc gggtagccag
    25441 gcggctgtct cgctggtcca ggtagtagcc cgttttgtgt ccgtgctgaa tatccaccag
    25501 cagcttcatg ccgtgttctt caatcggcag cagggcaggc ggcaactcgc cggtgacgag
    25561 gcccagggtc agctccatcc cttctttttt acgtaccgca acatcgctgc gatcgtaaat
    25621 cgcacattcc gggtacagcg tttgcagggc actaactaat gccgcgcgct gatattctgc
    25681 cccagcactc agcagttgca gcaccagaaa attaccgaaa cgatcgatag taatacccgg
    25741 caggccatca gattctccgg cgattaaacg atagctgtcg aggccatctt tttgcgccag
    25801 ccagtcacgc catttttgtg cttgttgcaa acggcgggaa aaaaaagcaa tgtcgataga
    25861 ctcagacggg tcaaacgtcc agacgcgcgc ccggatttgc gaagctggcg aataagcgcc
    25921 gcgtgctaac cattttccct gatgatcaac aatatcgatg gtttcaccga ggctggcttt
    25981 accttccatg cgggcaacgg ccccggaaaa gacccacgga tggcgacgaa gtaatgattt
    26041 ttcgcgccct ttggctaaca ctaaacgtac actcataatt tacttttctg tcgatgcaaa
    26101 gaaatgggcg tcattgtccg gagttttcca tggatttgca acgcgtccat taaggataag
    26161 gagagcagca atgtcgaaag tctgcataat tgcctgggtt tacgggcgag ttcagggcgt
    26221 aggatttcgc tacaccacac agtacgaagc gaaaagactg ggtttaaccg ggtacgccaa
    26281 aaatcttgac gatggcagcg tggaagtggt tgcctgcggt gaagaagggc aggtggaaaa
    26341 attaatgcag tggttaaaga gtggcggccc gcgttctgcg cgtgtagaac gggtgctcag
    26401 cgagccgcat catccctcgg gggaattaac cgattttcgt atccgttaaa tacattttac
    26461 cggttttggc aggccagcaa ttttggtggc ttgcttagcc ggacctttcg gaaacagtcg
    26521 gtataaatag cggctgttac ctttttcttc gccaaattta ttcgccatcg cttttaccag
    26581 catacgaatc gccggagaag tattgaattc cagatagaaa tcacgcacaa aacgcaccac
    26641 ttcccagtgt tccggtgaca gcgaaatccc ttcgttctct gcaatcacca ctgccagcgg
    26701 ctcactccac tggctgcttt ctttgagata gccttcggta tccgtttcta tctctttacc
    26761 ttcgaagatc agcataatta ttactacctt aatcagactg gcggcagtgt aacaaaaaac
    26821 aaagccccgc ataaagcgag gctatgaaag tgttagcggg tgagattaat cgcggctggc
    26881 gaagcccaga atgctcagca ggctgacgaa gatgttgtac agcgaaacat acaggctaac
    26941 cgtggcacga atatagttcg tttcaccgcc atgaacgatg ttgctggttt caaacaaaat
    27001 agcgccagag gagatcaaaa tgaagaccgc actgatcgcc agatgcagag caggcagctg
    27061 caggaagata ttagcaacca taccaatcag caccaccaca atacccgcca tcagcatacc
    27121 gccgaggaac gacatatctt tgcgggtggt cagcacatat gcagagcagc agaagaacac
    27181 taacgccgtt ccgcccagag ccatagcgat tacgtccccc attccggcag acagataggt
    27241 gttcagaata ggtccgagga tataacccag aaaaccggta aaggcgaatg cggagataat
    27301 cccggtcggc ttattcgccg ttttataggt caggaacatc aaaccataca tacccaccag
    27361 cgtcagaatc agacccggag atggcagcat cagcaccgta ctggcagttg cggtaatcgc
    27421 cgaaaaggcc agcgtcaggc tcagcagaaa ataggtatta cgcagcacct tatgggtgct
    27481 aagcagtgat gtacggtcat gtgaagaact aacaatacga tccattgagt cactctctat
    27541 gacagatgta attaattaag cagcataatg ataatgcgta agggcaccca gaagttttac
    27601 ccatctttac gcatttgatc tggaacaggt ttaacagcgg attatcaggt cattaagcaa
    27661 atataacgcc ctgagaattt cgacaggcaa aagaaaaagg ggttagcatt tagctaaccc
    27721 cttatcttat ttggcggaag cgcagagatt cgaactctgg aaccctttcg ggtcgccggt
    27781 tttcaagacc ggtgccttca accgctcggc cacacttccg gaatgacgcg cactataaac
    27841 atcccgatgc ggcgtgtaaa cccctaattt gtttgtttgc ctgaaaaaca gccaaaagtg
    27901 cattgatagc gtgaaataac agcagattga tcatttcatc accatgaatt ccttctcttt
    27961 tactcgttta gcaaccggct aaacatcccc accgcccggc aaaaagaaaa ataggtccat
    28021 ttttatcgct aaaagataaa tccacacagt ttgtattgtt ttgtgcaaaa gtttcactac
    28081 gctttattaa caatactttc tggcgacgtg cgccagtgca gaaggatgag ctttcgtttt
    28141 cagcatctca cgtgaagcga tggtttgcct tgctacaggg acgtcgcttg ccgaccataa
    28201 gcgcccggtg tcctgccggt gtcgcaagga gaagagacgt gcgatatgaa taacgaggaa
    28261 acattttacc aggccatgcg gcgtcagggc gttacccggc gcagctttct caaatattgt
    28321 agtctggctg ccacgtcgct gggattaggc gcgggaatgg caccaaagat tgcctgggcg
    28381 ctggagaaca aaccccgcat tccggtggta tggatccacg gtctggaatg cacctgctgt
    28441 accgaatctt ttatccgctc cgctcaccca ctggcgaagg acgtcatcct ttccctgatt
    28501 tccctcgatt atgacgatac tttgatggct gccgccggaa cccaggcgga agaagtcttc
    28561 gaagacatca tcacgcaata caatggcaaa tatatcctcg cagtagaagg taatccgccg
    28621 ctgggcgagc aggggatgtt ctgtatcagc agcggtcgac cgtttattga gaaactcaaa
    28681 cgtgccgctg ccggagccag cgcgattatc gcctggggaa cctgcgcgtc ctggggctgc
    28741 gtgcaggccg cgcggcccaa tccgacgcag gcaacgccta tcgacaaagt catcaccgac
    28801 aaacccatta tcaaagtacc tggctgcccg ccgatcccgg atgtgatgag cgccatcatt
    28861 acttacatgg tgacctttga tcgcttgcca gatgtcgaca gaatgggccg tccgctgatg
    28921 ttctatggtc agcgaatcca cgataaatgc tatcgccgcg cccacttcga cgccggagag
    28981 ttcgtccaga gttgggatga tgacgctgcc cgcaaaggtt actgcctgta caaaatgggc
    29041 tgcaaagggc ctaccaccta taacgcctgt tcctccacac gctggaatga tggcgtttct
    29101 ttcccaatcc agtctggtca cggctgcctg ggctgtgcgg aaaatggttt ctgggatcgc
    29161 ggttcgttct acagccgcgt ggtcgatatt ccgcaaatgg gtactcattc caccgccgat
    29221 accgtcggtt taaccgcgct tggcgtggtg gcagcggctg ttggtgtgca cgcagtcgcc
    29281 agcgccgttg accagcgcag acgtcataac cagcaaccta cagaaaccga acatcagcca
    29341 ggcaatgagg ataaacaggc atgagcactc agtacgaaac tcagggatac accatcaata
    29401 atgccggacg ccgcctggtg gtcgacccga ttacgcgcat cgaaggccac atgcgctgcg
    29461 aagtgaatat taacgatcag aatgtgatca ccaatgccgt ctcctgcggc accatgtttc
    29521 gcgggctgga gatcatccta caagggcgcg acccgcgcga tgcgtgggcg ttcgttgaac
    29581 gtatctgcgg cgtctgtact ggcgtacacg ccctggcttc ggtttacgcc atcgaagatg
    29641 ctatcggtat taaagtgccg gacaacgcca atatcatccg caacattatg ctggcaacgc
    29701 tctggtgcca cgatcatctg gtgcacttct atcagcttgc cgggatggac tggatcgatg
    29761 tgttagatgc gctgaaagcc gacccgcgga aaacctccga actggcgcaa agtctctcct
    29821 cttggccgaa atcatcccct ggctatttct tcgacgtaca aaaccgcctg aaaaaatttg
    29881 ttgaaggcgg gcagttgggg atcttccgca atggctactg ggggcacccg cagtacaaac
    29941 tgccgccaga agctaacctg atgggctttg cccactatct cgaagctctc gatttccagc
    30001 gtgaaattgt caaaatccac gcggtctttg gcggtaaaaa cccgcatcca aactggattg
    30061 tcggcgggat gccttgcgcc atcaacattg acgaaagcgg cgcggtcggg gcagtcaata
    30121 tggaacgcct gaacctggtg cagtcaatta tcacccgcac ggcggacttc attaacaacg
    30181 tgatgatccc cgacgcctta gccatcggtc agttcaacaa accgtggagc gaaatcggca
    30241 ctggtctttc tgataaatgc gttctcagct acggcgcatt cccggatatt gccaacgact
    30301 ttggcgagaa aagtctgctg atgcctggcg gcgcggtgat taacggcgac ttcaacaatg
    30361 tgctgccagt ggatttggtt gatccgcagc aggtgcagga gtttgtcgac cacgcctggt
    30421 atcgatatcc caacgatcag gtcgggcgtc atccgttcga tggcatcacc gacccgtggt
    30481 acaaccccgg cgatgtcaaa ggcagcgata ccaacattca gcagctgaat gaacaggaac
    30541 gctactcgtg gatcaaagcg ccacgctggc gcggtaacgc gatggaagtg gggccgctgg
    30601 cgcgcacgtt aatcgcttat cacaaaggcg atgctgcgac cgttgagtcg gtcgatcgca
    30661 tgatgtcggc gttgaacctg ccgctttccg gtatccagtc aacgttaggc cgcattttgt
    30721 gccgcgcgca cgaagcgcag tgggccgcag gtaagttgca gtatttcttc gacaagctga
    30781 tgaccaacct gaaaaacggc aatctcgcca ctgcttccac ggaaaaatgg gaacctgcaa
    30841 cctagccgac agagtgccgt ggtgtcggtt ttaccgaagc gccgcgcggg gcgttaggcc
    30901 actgggccgc cattcgcgat ggcaagattg atctctacca gtgcgtggtg ccgaccacct
    30961 ggaacgccag cccgcgcgat cccaaagggc agattggcgc ttatgaagcg gcgctgatga
    31021 acaccaaaat ggcgatcccc gagcaaccgc tggagatcct gcgtactctg cacagctttg
    31081 acccgtgcct cgcctgttca acacacgtgc tgggcgacga cggtagcgag ctgatctccg
    31141 tgcaggtgcg ttaacagcga aggagaatca tcatgcaaca gaaaagcgac aacgttgtca
    31201 gccactatgt ctttgaagcg ccagtccgca tctggcactg gttgacggtg ttatgcatgg
    31261 cggtgttgat ggtcaccgga tactttatcg gcaagccgct accttccgtc agcggcgagg
    31321 cgacgtatct gttctatatg ggctacatca ggttaattca cttcagcgcc gggatggttt
    31381 ttaccgtggt tttgctgatg cggatctact gggcttttgt tggcaatcga tactcccgcg
    31441 agctgtttat cgtgccggta tggcgtaaaa gctggtggca gggcgtgtgg tatgaaatcc
    31501 gctggtatct gtttctggca aaacgtccga gtgccgatat aggccataat cccatcgccc
    31561 aggcggcgat gttcggctat ttcctgatgt cggtctttat gatcatcact ggttttgcgc
    31621 tgtacagcga acacagccag tacgctattt ttgcgccgtt ccgttatgtg gtggaatttt
    31681 tctactggac gggtggcaac tcaatggaca ttcacagctg gcatcggctg gggatgtggc
    31741 tgattggcgc gtttgtgatc ggtcatgtct acatggcgct gcgtgaagac atcatgtccg
    31801 acggcacggt gatctccacc atggtcaacg gctaccgtag ccacaaattt ggcaaaataa
    31861 gtaacaagga gtgttcatga gcgagcaacg cgtggtggtc atggggctgg gcaacctgct
    31921 gtgggccgat gaaggcttcg gcgtgcgggt ggcggaacgg ctgtatgccc attaccactg
    31981 gcccgagtat gtggagattg tcgatggcgg tactcaggga ctgaacttgc tggggtatgt
    32041 cgaaagcgcc agccatctgt tgattctcga tgccattgac tacgggctgg aacctggaac
    32101 gctgcgaacc tatgccggag aacgcattcc ggcttatctc agcgcgaaga aaatgagcct
    32161 gcatcagaac agtttctccg aagtgctggc gctggcagat atccgcggac atctgccagc
    32221 acatattgcc ctcgtcgggc tgcaacccgc gatgctcgac gactacggcg gcagcctgag
    32281 cgaactggca cgggagcaac tgctcgctgc ggaacaatcg gcgctggcgc agttggctgc
    32341 gtggggaagt gtgccgcaac tggctaatga atcgcgttgt ctcaattatg actgtctgtc
    32401 gatggaaaat tacgaaggcg ttcgcttgcg ccagtaccgg atgacacagg aggagcaggg
    32461 atgagcaacg acacgccatt tgatgcgttg tggcaacgaa tgctggcgcg cggctggacg
    32521 ccggtcagtg aatcccgtct tgacgactgg cttacgcaag cgccagacgg cgtggtgtta
    32581 ttaagcagtg acccgaaacg cacgccagag gtcagcgata atccggtaat gattggcgaa
    32641 ttactgcacg agtttcccga ctatacatgg caggtggcga ttgctgacct tgagcagagc
    32701 gaagccatcg gcgatcgctt tggcgtcttt cgctttccta ccactttagt gtttaccggc
    32761 ggaaactatc gcggcgtgct gaatggtatt cacccgtggg cggaactgat aaacctgatg
    32821 cgcgggcttg tcgaaccgca gcaggagcgt gcctcatgag cgaaactttt ttccatctgc
    32881 tggggccagg aacgcaaccg aacgatgaca gtttcagcat gaatccactg ccgatcacct
    32941 gtcaggtgaa tgatgaaccg agtatggcgg ccctggagca atgtgctcac agcccgcagg
    33001 tgattgcgct gttaaacgag ttacaacatc aactaagcga acgccagccg ccgttgggcg
    33061 aggtgctggc agtcgatctg ttaaatctca acgccgacga tcgtcacttt atcaatacgc
    33121 ttctcgggga aggggaagtg tcagtgcgca ttcagcaggc tgacgacagt aaaagtgaaa
    33181 tacaggaggc gatcttctgc ggattatggc gggtgcgcag acgtcgcggc gaaaagttgc
    33241 tggaggacaa actggaggct ggctgcgcgc cgctggcgtt gtggcaggcg gcaacgcaaa
    33301 atctcttgcc gacagattcg ctgttaccgc cgcccattga tggcctgatg aatggcctac
    33361 cgttggcgca tgagttactg gcacatgtac gtaaccccga cgcgcagccg cacagcatta
    33421 atctgacgca attacccatc agcgaggctg atcggctttt tctctcacgt ctctgtgggc
    33481 cgggaaatat tcagattcgt accattggct atggcgagag ctatatcaac gccacggggt
    33541 tacgccatgt ctggcattta cgctgtacgg acaccttaaa aggcccgtta ctggaaagtt
    33601 atgaaatctg cccaataccg gaagtggtgc tggcagcgcc agaagatttg gtcgactctg
    33661 cgcagcggct tagcgaggta tgtcagtggc tggcggaagc tgcaccgacg taaaaagacg
    33721 gtaagtatcg ctttcagtct tatgaatatc gcaatcggcg aatacctctg gtcgtagagt
    33781 ttcaggataa agagggagat ctaacattat cgggttattt ttctctcttc gcctacagga
    33841 gtgcgcatgt gggatgtcat tgatttatcg cgctggcagt ttgctctgac cgcgctgtat
    33901 cactttttat ttgtacccct taccctgggg ctgatttttt tgctggctat catggaaacc
    33961 atttacgtgg tcaccggcaa aacaatctac cgcgatatga cgcgcttctg gggtaagctc
    34021 ttcggtatca attttgctct tggcgtggct accggcctga ccatggagtt tcagtttggt
    34081 actaactggg cattctattc caactatgtg ggcgatattt ttggcgcacc gctggcgatg
    34141 gaagcattaa tggccttctt cctcgaatcc acctttgtcg ggcttttctt cttcggctgg
    34201 cagcggctga ataaatacca gcacctgctg gtgacgtggc tggtggcgtt cggttcaaat
    34261 ctctctgcat tgtggatatt gaatgccaac ggttggatgc aatacccgac cggtgcgcat
    34321 tttgatatcg acaccctacg tatggagatg accagcttca gcgagctggt ctttaacccg
    34381 gtcagccagg tgaaatttgt gcacaccgta atggcgggct acgtgaccgg ggccatgttt
    34441 attatggcga tcagcgcctg gtatttactg cgcggacggg agcgcgatgt tgcattacgc
    34501 tcgtttgcca tcggttccgt cttcggtact ctggcgatta tcggcacgct acaactcggg
    34561 gacagttctg cgtatgaagt cgcgcaagta caaccggtaa aactggcggc gatggaaggg
    34621 gagtggcaaa cggaacctgc tcctgcaccg ttccatgtgg ttgcctggcc ggaacaggat
    34681 caagagcgta acgcctttgc catcaaaatt cccgcgctgt tagggatcct cgccactcac
    34741 tcattagata aacccgtgcc gggtctgaag aatttgatgg ctgaaaccta cccacgcttg
    34801 caacgcggac gtatggcctg gctgttaatg caggaaatat cgcaaggcaa tcgtgagccg
    34861 catgtgttgc aggcattccg ggaactggaa ggtgacctgg gctacggcat gttgctctcc
    34921 cgctatgcgc cggatatgaa tcatgtcaca gccgcacagt accaggcggc gatgcgtggc
    34981 gcgatacctc aggttgcgcc ggtattctgg agtttccgca tcatggtggg ctgtggttcc
    35041 ctgctgctac tggtgatgct gattgcgctt gtccagacgc tgcgtggcaa aatcgaccag
    35101 catcgctggg tgctgaaaat ggcgctctgg agtttgccgt tgccgtggat tgcgattgaa
    35161 gccgggtggt ttatgaccga gtttggtcgt cagccgtggg cgatacagga catcttaccg
    35221 acatactccg cgcactccgc tttaaccaca ggacaactgg ctttctcact gatcatgatc
    35281 gtagggcttt acaccctgtt cttaatcgcc gaagtctacc tgatgcagaa atatgcccgt
    35341 ctggggccga gcgcgatgca gagtgaacaa ccgacgcagc aacaggggta aagaagaaaa
    35401 tcatgtttga ttatgaaaca ttgcgcttca tctggtggct gctgattggc gtgatcctgg
    35461 tggtctttat gatctccgac ggatttgaca tggggatcgg ctgtctgctg ccgctggtgg
    35521 cgcgtaatga tgatgaacgc cggatagtga taaacagcgt tggtgcacac tgggaaggca
    35581 accaggtctg gttgatcctc gctggtgggg cattatttgc cgcctggccc agagtgtatg
    35641 cagcggcgtt ttccggcttt tatgtggcga tgatcctggt gctgtgctca ctgttcttcc
    35701 gcccgctggc ctttgattat cgcggaaaaa tcgccgatgc acgctggcgt aaaatgtggg
    35761 acgccggtct ggtcatcggc agtctggtgc cgccggtagt cttcggtatc gccttcggca
    35821 acttgttgct cggcgtgccg tttgccttca caccgcaatt acgcgtggag tatctcggca
    35881 gcttctggca actgctgacg ccattccctt tattgtgcgg attgctcagc cttgggatgg
    35941 taattttgca aggtggcgtc tggttacaac tgaaaactgt tggtgtgatt catctgagtt
    36001 cacagctggc caccaaacgc gctgcactgt tggtgatgct gtgctttttg ctggcgggtt
    36061 actggctgtg ggtcggtatt gatggctttg tactgctcgc ccaggatgct aacggtcctt
    36121 ccaatccgtt aatgaaactg gtggcagtgc tacctggtgc ctggatgaat aatttcgtcg
    36181 aatcgcccgt tttgtggatc ttcccgctgc tggggttctt ctgcccattg ctgacggtga
    36241 tggcgattta tcgtggtcgc ccaggttggg gatttttaat ggcgtcattg atgcaatttg
    36301 gcgtgatttt cacggcaggc atcacgctgt tccccttcgt catgccgtca agcgtgagtc
    36361 cgatctccag cctgacgttg tgggacagca cttccagcca gctgacgctg agcattatgt
    36421 tggtaatcgt gctgatattt ttgcccattg tgttgctcta cactctctgg agctactaca
    36481 aaatgtgggg gcgcatgaca acagaaactc tccgccgtaa cgaaaacgag ttgtactaag
    36541 gagcaggaac aatgtggtat ttactttggt tcgtcggcat tttgttgatg tgttcgctct
    36601 ccacccttgt gttggtatgg ctggacccgc gtctgaaaag ttaacgaacg taggcctgat
    36661 gcggcgcatt aacatcgcat caggcaatca ataatgtcag atatgaaaag cggaaacata
    36721 tcgatgaaag cgatcttaat cccattttta tctcttctga ttccgttaac ctcgcaatct
    36781 gcattcgctc agagtgagcc ggagctgaag ctggaaagtg tggtgattgt cagtcgtcat
    36841 ggtgtgcgtg ctccaaccaa ggccacgcaa ctgatgcagg atgtcacccc agacgcatgg
    36901 ccaacctggc cggtaaaact gggttggctg acaccgcgcg gtggtgagct aatcgcctat
    36961 ttcggacatt accaacgcca gcgtctggtg gccgacggat tgctggcaaa aaagggctgt
    37021 ccgcagcctg gtcaggtcgc gattattgct gatgtcgacg agcgtacccg taaaacaggc
    37081 gaagccttcg ccgccgggct ggcacctgac tgtgcaataa ccgtacatac ccaggcagat
    37141 acgtccagtc ccgatccgtt atttaatcct ctaaaaactg gcgtttgcca actggataat
    37201 gcgaacgtga ctgacgcgat cctcagcagg gcaggagggt caattgctga ctttaccggg
    37261 catcggcaaa cggcgtttcg cgaactggaa cgggtgctta attttccgca atcaaacttg
    37321 tgccttaaac gtgagaaaca ggacgaaagc tgttcattaa cgcaggcatt accatcggaa
    37381 ctcaaggtga gcgccgacaa tgtctcatta accggtgcgg taagcctcgc atcaatgctg
    37441 acggagatat ttctcctgca acaagcacag ggaatgccag agccggggtg gggaaggatc
    37501 accgattcac accagtggaa caccttgcta agtttgcata acgcgcaatt ttatttgcta
    37561 caacgcacgc cagaggttgc ccgcagccgc gccaccccgt tattggattt gatcatggca
    37621 gcgttgacgc cccatccacc gcaaaaacag gcgtatggtg tgacattacc cacttcagta
    37681 ctgtttattg ccggacacga tactaatctg gcaaatctcg gcggcgcact ggagctcaac
    37741 tggacgcttc ccggtcagcc ggataacacg ccgccaggtg gtgaactggt gtttgaacgc
    37801 tggcgtcggc taagcgataa cagccagtgg attcaggttt cgctggtctt ccagacttta
    37861 cagcagatgc gtgataaaac gccgctgtca ttaaatacgc cgcccggaga ggtgaaactg
    37921 accctggcag gatgtgaaga gcgaaatgcg cagggcatgt gttcgttggc aggttttacg
    37981 caaatcgtga atgaagcacg cataccggcg tgcagtttgt aatgcataaa aaagagcatt
    38041 cagttacctg aatgctctga ggctgatgac aaacgaagaa ctgtctaatg cgtagaccgg
    38101 aaaaggcgtt cacgccgcat ccggccactt tcagttttac tctttctcgg agtaactata
    38161 accgtaatag ttatagccgt aactgtaagc ggtgctggcg cgtttaatca caccattgag
    38221 gatagcgcct ttaatattga cgcctgcctg ttccagacgc tgcattgaca aactcacctc
    38281 tttggcggtg ttcaagccaa aacgcgcaac cagcaggctg gtgccaacag aacgccccac
    38341 gaccgcggca tcactcaccg ccagcatcgg cggcgtatcg acaatcacca gatcgtaatg
    38401 gtcgttcgcc cattccagta attgacgcat ccgatcgcgc atcagcagtt cagacgggtt
    38461 aggtggcacc tgaccgcgag taatcacatc aaagcctcct ttgccaaaat gctggatcac
    38521 tttgttgagc tcatctttac ctgccagata ttccgacaag ccatgttcat tactcacggt
    38581 aaacaggtta tgcgaataac cacggcgtaa gtcggcatca ataaataaca ctttttgatc
    38641 ggactgggcg atcaccgctg ccagagttga actgacaaac gttttaccac tgtctggcgt
    38701 cgcaccggtg atcatcagaa tgttattctc cgtctccatc atagcgaagt gcagactggt
    38761 tcgtagcgca cgtacggctt ccacagcaga atccgccggg ttatccaccg ccaggaaggg
    38821 gatattttta gtacgatggc gctgctgatt agaaaataaa tttttcttac gcagacgggt
    38881 acgtttatcc agccactcgg acattgggat agtggcataa acgctgatgc cgtgctcttc
    38941 cagttgttcc ggggcttcta caccacgacg caacatcgca cgcgccagca cggcacccac
    39001 cgaaataaac aggccaagaa taaaaccaag caccacgttc aacgcttttt tcggtttcac
    39061 tggctgcggc tgagtgactg ctgggtcgat aatccgcacg ttaccaatgg cactggattt
    39121 cgaaatactc aactcctgct ggcggttaag taattgcaga tataccgcac ggcccgcttc
    39181 tacgtcacgg cttaaacgta acacttcctg ttgagtggaa ggcattgccg atacccgctt
    39241 attcaggcgt ttgcgttctt gctccagcgt ctggcgtttt tccagcagcg cacgataagt
    39301 tgggtgatct ttcttataca gctgggagat ctctgcctcg cggaaagtca gctcattgag
    39361 ttgattatca acgttcacaa tctgctcaag aacggctttg gcttctaggt taaggtcaac
    39421 cgaatcgcgc tgctggcgat aaacgttgag tttttcttcc gcctggtcca gctcgctgcg
    39481 cacttcaggc aactggcgct gtaagaattc aaggctttgt gaatcctgcg ccgcctggcg
    39541 agcgatattc tgttgcaaat agttgttagc gatgctgttc agaatacgag taatcaactg
    39601 gggatcatca ccagtcatgg taagttccag catcccgctt tctttactgc gttcgctaac
    39661 ggtaaaggtt tcctgcaatg cgttaatcgc ttccagttcg gtacgctggc tcaggacaaa
    39721 ctgtgttcct ggtttggcct taatgtccgc gatagtcagc gcaacgccat ctttttccag
    39781 acgctggccg accataccat tgacggtgaa ctcttcacct tccagtgtat agtggccgtt
    39841 ttccccaacc gtgagtgtca gttgctgatc ctgaccattc agttgtggaa tatgcatcca
    39901 gctgatcgcc agctcacctg gtttttcttt ggttaatctc gcccagccgc gacccacaat
    39961 cggaaaatac ttctgctcaa ctatgtcgcg cagattcagt tcagcaatgg ttttaccgag
    40021 aatcatgcgc gattgcagca gttggatctc cggtgcagac tcgggcgatg agttagggat
    40081 catatcgctc aggccgctga gaatggcgtt gccctgtttt tgctcaacct ggaccagagt
    40141 atctgcctga taaattggtg tgcttaacag cgagtaagcg acagcgatca gcgtgaataa
    40201 cgcggttacg ctgataataa acttacggtg atcccataac tcgccgacca gacgaagcag
    40261 atcgatctca ttttcctgag tgctgcctgg tggcgtattc atatttttag ttgtcatcgt
    40321 attttataaa ctcaaattac cggctgaggc gcttcgccca ttcctgactg gcgcgctcca
    40381 acataccgta gacatgttca aatgcgtcct gacttttacg atagggatcc gggatctctt
    40441 tctgttccag ccattgccca aacagcattg tcttcccgcg gacctcggga gcgattgcgg
    40501 taacctgagc aatatgctct gactccattg ccagaatcag atcgtaattt cgtgccatct
    40561 cagcagtgag cttacgtccg gcatgtcctt caagagatac gccatgatta gcggcaacat
    40621 ctgctgctgt cgcatctgca gggtgtttta ccagaccatg aacacctgcc gatttgactt
    40681 ttacgcctgg cagacgttta cgcagcaagc gctcgccaat gggcgaacgg caaatgttgc
    40741 cggtacaaac caccaggatg gagttaaatt ttagttgggc catgtttgaa tccaccgtga
    40801 agtctctgtc aggtcatgca ccccggaaat cgtcggtacc agcagagaaa tcacacggtt
    40861 ccaacgagcc agcggcgcgg tagtgacata aacaatgtcg taaggttcaa gctggaattc
    40921 cgttccgagt atcattgccg atgcgtcctt cgcattcagc tgataaatat tggcgatttt
    40981 gccattctgc ttgttttgcg ttgcacgaat aacaaaaatc cccgtcgcat cagcgacatc
    41041 ctgattcata ccctcagcgt tacccagcgc ttccgccagg gtcataccgc tgcggtccat
    41101 tttcagcgtg ctctgtttac ccacttcacc catcacgaag actttcagat catcgttgcg
    41161 cggaataaat aaaatatcgc ccgggtgcag cagtttgttc tggcgcaagt cgccacgctg
    41221 catcagcgca tagaggttca ctttggtctt tacgccattc tgggtgagca ccacgttgcg
    41281 ccagtctgca tctgcggtca gaccgcccgc ggcgttaatc gcatccatga tggtcagagg
    41341 aatattggta atcggctgct gacccgattt gctgacttcg ccggtgacgt acgctttttg
    41401 cgatcggaac gccgcgacgc tgacatcgac ttgcgggctt tcaatgactg aatcgagacg
    41461 cgctgtaatc tcgttgcgta cttgggtaag tgttttcccg gccactttca ggcgaccaat
    41521 ataggggtag aaaatcgcac catcggcgtt aacccagttg ccggtatcac tggcgctacg
    41581 gtattgccct gcgggggtgg ttagttccgg gtgatcccag accgtaacca tcaacacatc
    41641 accaatcccg atacgatatt catagtttgc gatttgctgc tccagctccg ggttagcctg
    41701 cgacataatc ggtttggcgc gcaactgatc aatcaatcct ggcgtgacgg gatagatatt
    41761 gaccattttg tccaggtcat actgattgtc aggtagttca atgacatcct tgttcctggt
    41821 cgataaattc tgaccaggaa caagggtaca tgctgtcaaa gacagaacag ccaatgtcag
    41881 taccgaaaac ttaaaaatgt tctttttcat tggtttatat ttatcagtca atgtaatgtt
    41941 atgaattacg tcaggttatt gtccaaccgg tacttctcgc tctgccgtca ttggatagag
    42001 ctggtatttg cgtgacagca tttgtccacc atcacgcgtc aatggtgtcc acgaaaccac
    42061 cgcgcggttg cggttaggtc caatggtcat caagtcgaat ggaatcgaga tataaaaacc
    42121 tttactaaag ccgccttcgc cgtagtcatc tttagatacg ttactgattg ccgcccatac
    42181 ccctaccgcc acgccgctgt caaagcgttt ggcgacgtcg atcgttgctc ctttatcttt
    42241 tgccagatat tgcccaacgc taagtttcat cagtacgcca ttgagcgtcg gcgggttcca
    42301 gtaagccgtc acgaaaccag ttggcgtgga ataatcggtg aagcgcatca tgttgtccca
    42361 gtcacgttgc ttcacgtagt taacgtccac acccagcgcc cagctggcat ctagcgggcg
    42421 ataaagcagc tcggaaccga caccggcgta catcgtttcc agataaccgc catacacctg
    42481 gccatagaaa ccattgccta agtcggcaaa gtagttcgcc tgcaagttgt tgagataaac
    42541 gtcattgcga acgtaatcac ggatatgcgt gcgcacgcgg ggcagggtag agtccgcggg
    42601 caacagcgaa gacttaaact tgtcgtagtt gttgtaaata ttggtgaaaa taccgccatc
    42661 aagcagcagg tggtcggtaa accagtagcg ggcactggac atcagcccca gctggaacat
    42721 atagaaatct tccgggccgc ccagcgactg tgaaagtgtt gggttgaaag agtagctaaa
    42781 gcgatcttca cgaatacggt agccccgacc aaaggcagaa agatcttctg cttcaacacg
    42841 ttgttgttga agttgctctg attgaccagg cgctgtacca gccagctgtt tgcgcaggct
    42901 ggctacgtcg gtttcggtag tcaccatcgc catatgctcg cgcttttgcg tcacgctaat
    42961 cttctcaacg ccttgcggca ggttattcac cagaatccgg ttggcacgat cgaccgcttc
    43021 gcgagaatca cggtatttgt attgttgacc agacatatac agcgtcttat cgcgcagctg
    43081 aatttccggt gcttcaaaac ctgcgttgta cttcagcgcg gtaagttggt tcgctactgt
    43141 ggtgtactgc aatccttcag attcaggcgc aggttgatat gccggttttg gcgtatcgcg
    43201 cagggcaggg cgcagatcgt tgaaattggt ccgtaacgtg aagccaaaca tcaacgtgtt
    43261 accgcgttca taactcaggt tgagatctgc ccagctggca gcgcgataaa ctgcgccgac
    43321 gttgaaatgg cttgcctgag gcagtttgcc agcgaaatca ttctggtaat tgttgccatc
    43381 gtattcgagt ttcagacgca ggggattcca cggcgtttga tactcaatgc cgccaaagat
    43441 ggaagccggg ccacgaaaga tatcgctaaa gctgatatcg cccgcatcgt gagactctgc
    43501 gcgatgacaa tatttatcgt tcacccggca aaacgggttg gtaatattgc ccgcattacc
    43561 ggcgtagccc catgccatcc cgagggtgaa atcaaatggc cccgcttgct tgctggccac
    43621 cagatactca ccgtcaaaca gacccgtacc agcaatatca cgtttaccaa acgccacttg
    43681 cggtagccag taaccttctt cccacaggcg aagtttaaaa tcgaatgatt tgtctttgta
    43741 tgactgatcg ccgctgaaat cttcccactg gctatatttg cgtgtgcgca catccgtata
    43801 acgaatggtg ccttccagcc atgggaacag cgccacggag gtggagtaga accggtattg
    43861 atcgttatcc cggtagttga cgctgaattc accttccggt gcgatgcgcg catttggcat
    43921 ctgcaacaat cctgtgccgc caaagtctga ttgcgacgaa cccagcggat ccgggtaggt
    43981 taatacttca gcatgacagg cactggagac ggcaatggcc aggcagctta aaagataaga
    44041 attcttcttc atcagtcagg aacacgttgc gttaaaacag acacaatctg gtcatttaaa
    44101 tcagcatatt tttcgggcag tacatgcgca gaaaagccca accaaagctg gctgccaggc
    44161 ggtgggtcca catgtctttt attccacagg gcaacagggg cgacgacagt ctcaccctca
    44221 ggcgtaatca ccataacgtt attcttgtcc gcgcctgcaa ggcgggggtg atcttgcaga
    44281 taatcggtaa cgctacggcc tgcctgccag ggcaattgcc cggccccaga aaccgcaccc
    44341 agcagagtaa tagtcaccgg acgctgtacg gtatagagcg tatagtcccc gaccaacggg
    44401 ggattgctgt tctcatccac tcgaacaaaa tcagggtcca gtttgaccgg taagcgacca
    44461 gtgatattga gattgagtaa ctgctggcgc acagatttaa tcgttgccgc cacatcgtcg
    44521 tcagcttctg cttcccagga agcaagctgt gccatgacat gttggtagtc tttaagcgct
    44581 ttagctttcg ccgcagagtc ggtcagcaag gcaccaggcc accagaggcg atcgcgcagt
    44641 tgcggttgtg tcaccaactg gacaacattt tccatggggc caacggaaag tgtctgttgt
    44701 tcgccaggca gataaatagt caccgttcct tgcgcaaagg catggggtgt cattacgtaa
    44761 agtacgctgg caatgaaata cgactgtaat ttattcatgg tgcgggttta agaaacgtca
    44821 tttctacggg aataacccct gcgcctaaca tttgccgact ttgacgaacc tggccagtag
    44881 ccgaatcgac ccagaatgtg ttttgccact gtttttctgg ccgcgtggaa gtcacctctt
    44941 cctgccagac attacacagc acattgcgac ccgcaatatt cagcgtctct aatccagcaa
    45001 aggaaaaagt agaactcagg gtcgcagagc gaaaatggtt gtcttcactc cagagaatat
    45061 cgcgcgtcca gcggctgcca tcttttattg ccagcgcgtt acgcagaggg tcctgcccgg
    45121 aatttgtcac ttccagcaga ttattattaa gtttgacggt ttttaaaaga cgtccattgt
    45181 gggtaaccag catggcgtta tcctgggata accatttgct ttgttcttgt tctatatatc
    45241 ccagtaccac aaagattcgt tgcccttcat taaggcgtaa atacatcgtg gaataaggca
    45301 acgcctggat ctgctgatcc gctacggtga tatcctgatt atcgaaaagg ctggcgcgaa
    45361 atgtatcgac catactttgc tggctgtgcg tacatcccgc aagaaatagt gcgaaaatcg
    45421 ataaaataag agggcgcacc tttaatcctt gaaaggttaa ttaaaaaaat gcaaatcttt
    45481 aaagcccggt acgaatatcg caaccgggta tcatttaact ttaatttcga cgcgttaaag
    45541 cagtattagc gtgtagtgga cgtggtggtt gaggtgttat gagaaccgcc atcgccactg
    45601 tcatttgctg caacaactgc caacgtcgcc acaccaacag ccgttgccac accgacaact
    45661 gtcgccgtgc cgacagcgcc agcagccacg ccaccagtgg tagtagcagt cgtggttgcg
    45721 ccggtagtgg tcgttgtggt gccagtagta cccgtcgttg cctcggtccc gtttgttgct
    45781 tcaggggcag caaacgcagc tggcgtcgtc aacatgaagg ccataaggat tgcagaaagc
    45841 ttgtgtttca taacttttcc tttattcatc gcatggacaa tacgtacgct tgagatcagg
    45901 attgtctact ttgagtataa catccttgtg aaatctccct ttcctcatac cgcttgcact
    45961 atatactgcg ggtaggaaag gcgcgcagag ggaaatataa gattgttaac tatccccctc
    46021 tggaaatacg acttgtgagc ataagataaa tcttagcaaa atacaaatta aagataagga
    46081 aattgttacg aaagctatta attattgcta gtaatatagt ttcaagtggt actataactg
    46141 gctgttaaat ggcatttata actattaggt gcagagatat tcgcttaaat ggattagttt
    46201 ctttgtggaa aagtaactga tgttataata tatgatgttg actattgcac tattccaata
    46261 aaaccagtca gctttaaaca agcagcgtca tattaagaga gataaacatt tgccgctgtt
    46321 ggtcctcgca ggccatttac gcggcaaaat tccacacgta atcctggtat aagcacttct
    46381 gcgtcgcggg gagtgaatgc ggaaatatgg acctgaactt ctttacgacc gtcggagggg
    46441 ataatgaatc ctttgccgct tttgcgatca aaggttttga caattcctgt cattttacgg
    46501 gacaaacaaa ttccttactg aaaatactgc gctgcactat acggggttaa taaaataaag
    46561 ccagcgatat ttaagaccgc cggacggcta aaataaaatt tgcttaatct caattatcat
    46621 gcgttaatag ctgcgtcggt ttgaaagaca gacagcatac aaagtagttt actaaagcag
    46681 ttctcattat caggcattat ccccttcttt tgagtctctc tcctgaacac taagtagttt
    46741 ctgtattaaa gccctgtttg ccgaaaggcc caaaatgaag gaagtaaaat atgtctaata
    46801 aaatgactgg tttagtaaaa tggtttaacg cagataaagg ttttggcttt atcactcctg
    46861 atgatggcag caaagacgtt ttcgtccatt tcactgccat ccagagcaat gaattccgca
    46921 cgctgaacga aaatcagaaa gttgaatttt ctattgagca ggggcaacgt ggccccgcgg
    46981 cagcgaacgt tgttacgctc taaggttgcc gttattactc aacatctcca tttccgctgt
    47041 ccatgttgtc atggttcaca gtaccgcaca tcggcattcg atgtgacgga gcgaaatcct
    47101 ttgggcgcta agtgtatttt ttgtaaatcg acgatgatca cctttgataa cgtcacgctg
    47161 caaatacgca ctgaccatgc gccgctggat ttcacaaaat aatatcaggc tccctcg
//
LOCUS       DAJVUJ010000008        43882 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.008, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000008 DAJVUJ010000000
VERSION     DAJVUJ010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43882
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.008"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            86..628
                     /gene="lpfE"
                     /locus_tag="OQQ48_000500"
     CDS             86..628
                     /gene="lpfE"
                     /locus_tag="OQQ48_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfE"
                     /protein_id="HCS1951382.1"
                     /translation="MKRTILALCLLPLSFSSFAATTDLGPKGTLKFTLKISQGACELV
                     KDSVEVDMGTAVLKKPVRIGTEINPTPFSIGLKNCSEVVRAYVTMDGTPNADDSNLFA
                     LDAGGATGVGLKIKTAAGVQQFPENTTPIPVEFAINGDGAHQLNYIASYVPIRADATI
                     GRADATVDFTVQYSTNNFTS"
     gene            868..2559
                     /gene="eptB"
                     /locus_tag="OQQ48_000501"
     CDS             868..2559
                     /gene="eptB"
                     /locus_tag="OQQ48_000501"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HCS1951383.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQAKEAAAN"
     gene            2651..2727
                     /locus_tag="OQQ48_000502"
     tRNA            2651..2727
                     /locus_tag="OQQ48_000502"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2685..2687,aa:Pro,seq:cgg)
     gene            3471..5078
                     /gene="dppA"
                     /locus_tag="OQQ48_000503"
                     /pseudo
     CDS             3471..5078
                     /gene="dppA"
                     /locus_tag="OQQ48_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
     gene            5386..6405
                     /gene="dppB"
                     /locus_tag="OQQ48_000504"
     CDS             5386..6405
                     /gene="dppB"
                     /locus_tag="OQQ48_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HCS1951384.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPASGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            6415..7317
                     /gene="dppC"
                     /locus_tag="OQQ48_000505"
     CDS             6415..7317
                     /gene="dppC"
                     /locus_tag="OQQ48_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HCS1951385.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDMLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDIAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            7328..8311
                     /gene="dppD"
                     /locus_tag="OQQ48_000506"
     CDS             7328..8311
                     /gene="dppD"
                     /locus_tag="OQQ48_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HCS1951386.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLVFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            8308..9312
                     /gene="dppF"
                     /locus_tag="OQQ48_000507"
     CDS             8308..9312
                     /gene="dppF"
                     /locus_tag="OQQ48_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HCS1951387.1"
                     /translation="MSTQEATLQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLSGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(9342..10613)
                     /locus_tag="OQQ48_000508"
     CDS             complement(9342..10613)
                     /locus_tag="OQQ48_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCS1951388.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            11089..11196
                     /locus_tag="OQQ48_000509"
     CDS             11089..11196
                     /locus_tag="OQQ48_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCS1951389.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(11283..12962)
                     /gene="bcsG"
                     /locus_tag="OQQ48_000510"
     CDS             complement(11283..12962)
                     /gene="bcsG"
                     /locus_tag="OQQ48_000510"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HCS1951390.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVETTGGAPVVGDMPAQTTPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTTVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(12959..13150)
                     /gene="bcsF"
                     /locus_tag="OQQ48_000511"
     CDS             complement(12959..13150)
                     /gene="bcsF"
                     /locus_tag="OQQ48_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HCS1951391.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(13147..14718)
                     /gene="bcsE"
                     /locus_tag="OQQ48_000512"
     CDS             complement(13147..14718)
                     /gene="bcsE"
                     /locus_tag="OQQ48_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HCS1951392.1"
                     /translation="MRDIVGPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDYQGPEKIKLFSMLNHEKGLYYLTRDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLTHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            14991..15179
                     /locus_tag="OQQ48_000513"
     CDS             14991..15179
                     /locus_tag="OQQ48_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HCS1951393.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            15191..15943
                     /gene="bcsQ"
                     /locus_tag="OQQ48_000514"
     CDS             15191..15943
                     /gene="bcsQ"
                     /locus_tag="OQQ48_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HCS1951394.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHRQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAIAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            15940..18552
                     /gene="bcsA"
                     /locus_tag="OQQ48_000515"
     CDS             15940..18552
                     /gene="bcsA"
                     /locus_tag="OQQ48_000515"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HCS1951395.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPLDPVRYLIQTCWLLIGTSRKETPKPRRR
                     AFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSLIL
                     ALICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRYTS
                     TLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVDIF
                     VPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIARTTH
                     EHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHFFS
                     PDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIAVE
                     TVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLDNP
                     LTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFVLP
                     HMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGGLV
                     EEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVLGG
                     AVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQILEG
                     QKVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWALWQ
                     DSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFILRRPER
                     SETAQPSDQALAQQ"
     gene            18563..20902
                     /gene="bcsB"
                     /locus_tag="OQQ48_000516"
     CDS             18563..20902
                     /gene="bcsB"
                     /locus_tag="OQQ48_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HCS1951396.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYMPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETRSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERVWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            20909..22015
                     /gene="bcsZ"
                     /locus_tag="OQQ48_000517"
     CDS             20909..22015
                     /gene="bcsZ"
                     /locus_tag="OQQ48_000517"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HCS1951397.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            21997..25470
                     /gene="bcsC"
                     /locus_tag="OQQ48_000518"
                     /pseudo
     CDS             21997..25470
                     /gene="bcsC"
                     /locus_tag="OQQ48_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
     gene            25552..27540
                     /gene="hmsP"
                     /locus_tag="OQQ48_000519"
     CDS             25552..27540
                     /gene="hmsP"
                     /locus_tag="OQQ48_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HCS1951398.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            27723..29009
                     /gene="dctA"
                     /locus_tag="OQQ48_000520"
     CDS             27723..29009
                     /gene="dctA"
                     /locus_tag="OQQ48_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HCS1951399.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            29229..30725
                     /locus_tag="OQQ48_000521"
     CDS             29229..30725
                     /locus_tag="OQQ48_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCS1951400.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPAQ"
     gene            complement(30821..31750)
                     /gene="kdgK"
                     /locus_tag="OQQ48_000522"
     CDS             complement(30821..31750)
                     /gene="kdgK"
                     /locus_tag="OQQ48_000522"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HCS1951401.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDISLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSTAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            31982..32749
                     /gene="pdeH"
                     /locus_tag="OQQ48_000523"
     CDS             31982..32749
                     /gene="pdeH"
                     /locus_tag="OQQ48_000523"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HCS1951402.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFIEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLGDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            32819..34879
                     /locus_tag="OQQ48_000524"
     CDS             32819..34879
                     /locus_tag="OQQ48_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCS1951403.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(35113..36435)
                     /locus_tag="OQQ48_000525"
     CDS             complement(35113..36435)
                     /locus_tag="OQQ48_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HCS1951404.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLLILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETQHQSL"
     gene            complement(36846..37859)
                     /gene="yhjD"
                     /locus_tag="OQQ48_000526"
     CDS             complement(36846..37859)
                     /gene="yhjD"
                     /locus_tag="OQQ48_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HCS1951405.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(37908..38807)
                     /locus_tag="OQQ48_000527"
     CDS             complement(37908..38807)
                     /locus_tag="OQQ48_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1951406.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLNELDGLFQQDATSISGKLRIDIPPGI
                     AKNLLLPRLSEFLYLHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            39327..39929
                     /locus_tag="OQQ48_000528"
     CDS             39327..39929
                     /locus_tag="OQQ48_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCS1951407.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            complement(39980..41629)
                     /locus_tag="OQQ48_000529"
     CDS             complement(39980..41629)
                     /locus_tag="OQQ48_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCS1951408.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVKRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKLI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            42033..43430
                     /gene="ccp"
                     /locus_tag="OQQ48_000530"
     CDS             42033..43430
                     /gene="ccp"
                     /locus_tag="OQQ48_000530"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HCS1951409.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            43641..>43882
                     /locus_tag="OQQ48_000531"
     CDS             43641..>43882
                     /locus_tag="OQQ48_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCS1951410.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
BASE COUNT        10545 a        11003 c        11919 g        10415 t
ORIGIN      
        1 atcccgttag cactaccgga aaattacccg ctgccgggga ttatagcggt attgctacca
       61 tacatgtcga actggagtaa cagtcatgaa gcgaacaata cttgcgttat gcctgctgcc
      121 tctttccttt tcgtcattcg cggcaaccac ggatttaggg ccgaaaggaa cattgaaatt
      181 cacccttaaa atcagccagg gtgcttgcga actggtgaaa gacagcgttg aggttgacat
      241 gggcaccgcg gttctcaaaa agccggtaag aatcggaacg gaaattaatc ccactccttt
      301 ttccatcggc ctgaaaaact gtagcgaagt ggtcagagcc tacgtgacta tggacggtac
      361 acccaatgca gacgactcaa atctttttgc gctggatgcg ggtggcgcta caggcgttgg
      421 gctgaaaatc aaaaccgctg ctggagtaca acagttcccg gaaaatacaa cgccgatacc
      481 ggttgagttt gcaattaacg gtgatggcgc acatcaactg aactatatcg ccagctacgt
      541 gcctattcgc gccgatgcaa ctattggtcg cgcagatgca acggtcgact ttactgtaca
      601 gtacagtacg aataatttca catcttaagg gccagttaac tggccctttt tactgctttt
      661 tgtaacgcta aagtctcttt tcaaacttgc atttttgtaa atttgtgctt catgcacact
      721 ctttccccac actttttcgc tttgctgtgg tctacttatt cgcgcgtgta gattttactt
      781 atctgactac ctccgcactt ttcccctgcc gggcctgaaa agccgctaag cagggtgtta
      841 tcacctgttt gtccagggtt tgtttgcatg agatacatca aatcgattac acagcagaag
      901 ctgagctttt tgcttgcaat ctatattggc ctttttatga atggcgcggt tttttatcgc
      961 cgcttcggca gctatgcgca cgattttacc gtctggaaag gcgtttctgc tgttgttgaa
     1021 ctggccgcca ccgtactggt gaccttcttt ttactacgtc ttctatcgct gtttggccgc
     1081 cgcagctggc gtattctggc gtcgctggtg gtgctctttt ccgcaggtgc cagctattac
     1141 atgaccttcc ttaatgtggt cattggttat ggcatcatcg cttccgtcat gaccaccgat
     1201 atcgacctgt caaaagaagt tgttggtctg aactttattc tctggttaat cgccgttagt
     1261 gcattgcctc ttatccttat ctggaataac cgctgtcgct acaccttgct ccgacaactg
     1321 cgaaccccag ggcagcgtat tcgcagcctg gcggtcgtcg tactggcggg tattatggtt
     1381 tgggcaccga ttcgtttgct ggatatccag cagaagaaag tggagagggc gaccggcgtt
     1441 gatttgccga gttatggcgg tgtcgtagcg aactcttatc tgccatcaaa ctggctttct
     1501 gcgttggggc tgtatgcctg ggcgcgggtc gatgaatctt ccgataataa ttcattgctt
     1561 aatccggcga agaagtttac ataccaggct ccacaaaacg ttgatgatac ttatgtcgtg
     1621 tttatcatcg gtgaaaccac gcgttgggac catatgggta ttttcggcta tgagcgtaat
     1681 accacgccga aactggccca ggagaaaaat ctggcggcgt tccgtggtta ctcctgtgat
     1741 accgcaacca aactctcact gcgttgcatg tttgtacgtc aggggggcgc ggaagataat
     1801 ccgcagcgca cattaaaaga acagaacatt ttcgcggttc tgaagcagtt aggattcagt
     1861 tctgacctct acgctatgca gagcgaaatg tggttctaca gcaacacgat ggcggacaac
     1921 attgcttatc gtgagcagat tggtgcggag ccacgtaatc gtggcaagcc ggtagatgat
     1981 atgttgctgg tagacgaaat gcagcaatcg ctagggcgca acccggatgg taagcatctg
     2041 atcattctgc ataccaaagg ttcgcatttt aactacaccc agcgttatcc gcgtagcttc
     2101 gcgcagtgga agccggaatg tattggtgtt gatagcggct gtaccaaagc gcagatgatc
     2161 aactcctatg acaactcggt gacctatgtg gatcacttta tctccagcgt gattgatcag
     2221 gttcgcgata agaaagcgat tgtgttctac gcagctgacc acggtgagtc aattaatgaa
     2281 cgcgagcacc tgcacggcac gccgcgtgaa ttggcaccgc cggagcagtt ccgcgtaccg
     2341 atgatggtct ggatgtcaga taaatatctg gaaaatccgg ccaatgcgca ggcatttgcg
     2401 cagctgaaaa aagaagccga catgaaagtg ccacgccgtc acgtagagct gtacgatacc
     2461 atcatgggtt gtcttggcta tacttcaccg gatggtggaa ttaacgaaaa caacaactgg
     2521 tgtcacatcc cgcaggcaaa agaggcagcg gctaactaag cggcctgctg actttctcgc
     2581 cgatcaaaag gcattttgct attaagggat tgacgagggc gtatctgcgc agtaagatgc
     2641 gccccgcatt cggtgattgg cgcagcctgg tagcgcactt cgttcgggac gaaggggtcg
     2701 gaggttcgaa tcctctatca ccgaccaaat tcgaaaagcc tgctcagcga gcaggctttt
     2761 ttgcatttga agcccatgag gatgagaacc tccgggggct gggaggttcg actcgacgta
     2821 ggcctgataa gacgcgccag cgtcgcatca ggcaacggca ccgcacttat tgtcggatgc
     2881 ggcgcgaccg ccttattcga ccaacacacc acacattttc ctgccaactt tgcgccccat
     2941 atctcactat ccattaacgg atttgtgaca gaaaccattg tcttttttta tatttgctta
     3001 aatctttttt tgcgttactt atggctggcg ctggcatttt ccgctgtgcg ttttagcgtt
     3061 catggtatta atcgctcaga tatccacctt ttcctcagat ttttttcatt aaatgcataa
     3121 aacttaatca ccaaatgttg ctaaatatta agctgattgt tctggttatg ttggagtagc
     3181 acaaatttct ctgctgcaaa tagctgtttg aagtttttta atctttgttt gtgggttctc
     3241 accgttggtg taaatcccgg ttggctgtat tgacgttttc acattctgtt gacagattgt
     3301 aggtcacgag gggcatttta tggaggatcc gcactgttac actgatgtta attagtacgg
     3361 catccccacc tcataacgtt gacccgaccg ggcaaaaaac aaaaaaggtc aggtagcgac
     3421 aacccactgc aaagggttaa aacaacaaac atcacaattg gagcagaata atgcgtattt
     3481 ccttgaaaaa gtcagggatg ctgaagcttg gtctcagcct ggtggctatg accgtcgcag
     3541 caagtgttca ggctaaaact ctggtttatt gctcagaagg ctctccggaa gggtttaacc
     3601 cgcagctgtt tacctccggc accacctatg acgcctcttc cgtaccgctt tataaccgcc
     3661 tggttgaatt taaaatcggc accactgaag tgataccggg cctcgctgaa aagtgggaag
     3721 tcagctaaga cggtaaaatc tataccttcc atctgcgtaa aggtgtgaag tggcacgaca
     3781 ataaagaatt caaaccgacg cgtgaactga acgccgatga cgtggtgttc tcgttcgacc
     3841 gtcagaaaaa cgcgcaaaac ccgtaccata aagtctctgg cggcagctac gaatacttcg
     3901 aaggcatggg cttgccggag ctgatcagtg aagtgaaaaa ggtggacgac aacacagttc
     3961 agttcgtact gactcgcccg gaagcgccgt tcctcgctga tctggcaatg gacttcgcct
     4021 ctattctgtc aaaagaatat gctgatgcga tgatgaaagc cggtacaccg gaaaaactgg
     4081 acctcaaccc aatcggaacc ggtccgttcc agttacagca gtaccaaaaa gattcccgta
     4141 tccgctacaa agcgtttgat ggctactggg gcaccaaacc gcagatcgat acgctggtct
     4201 tctctattac ccctgatgct tccgtgcgtt acgcgaaatt gcagaagaat gaatgccagg
     4261 tgatgcagta cccgaacccg gcagatatcg ctcgcatgaa gcaggataaa tccatcaacc
     4321 tgatggaaat gccggggctg aacgtcggct acctctcgta taacgtgcag aaaaaaccgc
     4381 tggatgacgt gaaagttcgc caggcactga cctacgcggt gaacaaagac gcgatcatca
     4441 aagcggttta tcagggcgcg ggtgtatcag cgaaaaacct gatcccgcca accatgtggg
     4501 gctataacga cgacgttcag gattacacct acgatcctga aaaagcgaaa gccttgctga
     4561 aagaagcggg tctggaaaaa ggtttctcca tcgacctgtg ggcgatgccg gtacaacgtc
     4621 cgtataaccc gaacgctcgc tgcatggcgg agatgattca ggcagactgg gcgaaagtgg
     4681 gcgtgcaggc caaaatcgtc acctacgaat ggggtgagta cctcaagcgt gcgaaagatg
     4741 gcgaacatca gacggtaatg atgggctgga ctggcgataa cggggacccg gataacttct
     4801 tcgccaccct gttcagctgc gccgcctctg aacaaggttc taactactca aaatggtgct
     4861 acaaaccgtt tgaagatctg attcaaccgg cgcgtgctac cgacgaccat aacaaacgcg
     4921 ttgaactgta caagcaatcg caggtggtga tgcacgatca ggctccggca ctgatcatcg
     4981 ctcactccac cgtgtttgaa ccggtacgca aagaagtcaa aggctatgtg gttgatccat
     5041 taggcaaaca tcacttcgaa aatgtctcta tcgaataatt aaaagccata caagactgat
     5101 ggtaaaggca aaactgcctg atgtgctccg cttatcaggc ctacgaaaat tctgcaatgt
     5161 attgaattta cacgattttg taggccggat aaggcgttaa cgccgcatcc ggcataaaca
     5221 aagcgcactt tgtcaacaat ctgtataccc ggtggcgctg tgcctctgcg tgcagcgcca
     5281 tccggcagca atacttcatt ctctgtccga caagggcggg aattgatttg tgagcaatac
     5341 agacacgcag ttccaggctg cgggtcacta tagagaatcc gggttatgtt gcagtttatt
     5401 ctccgacgtt tgggactcgt catccccacg tttatcggta ttacccttct cacatttgcc
     5461 tttgtccaca tgatcccggg cgatccggtg atgatcatgg caggcgaacg tgggatctcc
     5521 cctgagcgtc acgcgcagct gctggctgaa ctcggcttag ataaaccgat gtggcagcag
     5581 tatctccatt acatttgggg cgtaatgcac ggcgatctag gcatttcaat gaaaagccga
     5641 attccagtat gggaagagtt cgtgccgcgc tttcaggcca cgctggaact tggcgtctgc
     5701 gcgatgattt ttgctacggc agtcggtatt ccggttggtg tgctggctgc ggttaaacgc
     5761 ggttccattt tcgatcacac tgcagttggc ctggcgctga ccggctactc gatgccgatc
     5821 ttctggtggg gcatgatgct gatcatgctg gtttcggtgc actggaacct gacgcccgcc
     5881 tccggtcgcg tgagcgatat ggtgttcctc gatgactcca atccgttaac cggttttatg
     5941 ctaatcgaca ccgccatctg gggtgaagac ggcaacttta tcgatgccgt cgcccatatg
     6001 atcttgcctg ccattgtgct gggtactatt ccgctggcgg tcattgtgcg tatgacacgc
     6061 tcctcgatgc tggaagtgct gggcgaggat tacatccgca ccgcgcgcgc caaagggcta
     6121 acccgcatgc gggtgattat cgtccatgcg ctgcgtaacg cgatgctgcc ggtggtgacc
     6181 gttatcggcc tgcaggtggg aacattgctg gcgggggcga ttctgaccga aaccatcttc
     6241 tcgtggcccg gtctgggacg ctggttgatt gacgcactgc aacgccgcga ctatccggta
     6301 gtgcagggcg gcgtattgct ggtggcgacg atgattatcc tcgtcaactt gctggtcgat
     6361 ctgctgtacg gcgtggtgaa cccgcgtatt cgtcataaga agtaagggga catcatgtca
     6421 caggttactg aaaataaagt gattagcgca ccggtgccga tgaccccgtt acaggagttc
     6481 tggcactatt ttaaacgcaa caaaggcgcg gtcgtcgggc tggtttacgt cgtcatcgtg
     6541 ctgttcatcg cgatctttgc caactggatt gcaccctata acccggcgga acagttccgc
     6601 gatgcactgc tcgccccgcc agcctggcag gaaggcggca gcatggcgca cttgctgggc
     6661 accgatgacg taggccgtga tatgctgtcg cgcctgatgt acggtgcgcg cctgtcgctg
     6721 ctggttggct gtctggtagt tgtgttatcg ctgattatgg gcgttattct cggcctgatc
     6781 gccggttact ttggcggcct agtcgataac atcattatgc gcgtggtcga tatcatgctg
     6841 gcgctgccaa gtctgctgct ggcgctggtg ctggtggcaa ttttcggccc gtcgattggt
     6901 aacgccgcgc tggcactgac cttcgttgcc ttgccgcact atgtgcgctt aacccgcgcc
     6961 gccgtgctgg tggaagttaa ccgcgattac gtcaccgcgt ctcgcgtggc gggtgccggg
     7021 gcgatgcgtc agatgtttat taacatcttc ccgaactgcc ttgcgccgct gattgttcag
     7081 gcgtcgctcg gtttctctaa cgccattctc gatatagctg ctcttggttt cctcggcatg
     7141 ggggcacagc cgccaacgcc ggagtggggc accatgctct ccgacgtgtt gcagttcgcg
     7201 caaagcgcct ggtgggtcgt gaccttcccg ggtctggcga tcctgctgac ggtgctggca
     7261 tttaacctga tgggtgacgg tctgcgtgac gcgctcgatc ccaaactgaa gcagtaagag
     7321 gttcgagatg gcgttattaa atgtagataa attatcggtg catttcggcg acgaaagcgc
     7381 accgttccgc gccgtagacc gcatcagcta cagcgtaaaa cagggcgaag tggttgggat
     7441 tgtgggtgag tccggctccg gtaagtcggt cagttccctg gcgattatgg ggctgattga
     7501 ttatccgggc cgcgtaatgg cggaaaaact ggtgtttaac ggtcaggatt tgcagcgtat
     7561 atcagaaaaa gagcgccgca acctggtggg tgccgaagtg gcgatgatct tccaggaccc
     7621 gatgaccagc cttaacccgt gctacaccgt gggtttccag atcatggaag cgattaaggt
     7681 gcatcagggc ggcaacaaaa gtacccgccg tcagcgggcg attgacctgc tgaatcaggt
     7741 cggtattccc gatccggctt cgcgtctgga tgtttacccg catcagcttt ccggcggcat
     7801 gagccagcgc gtgatgatcg ccatggcgat tgcctgtcga ccaaaactgc tgattgccga
     7861 tgagccgacc accgcgctgg acgtgaccat tcaggcgcaa atcatcgaac tactgctgga
     7921 gctacagcag aaagagaaca tggcgctggt gttgattacc catgacctgg cgctggtggc
     7981 ggaagctgcg cataaaatca ttgtgatgta tgcaggccag gtggtggaaa cgggcgatgc
     8041 gcacgccatc ttccatgcgc cgcgtcaccc gtatactcag gcattgctgc gtgcgctgcc
     8101 agaatttgct caggacaaag aacgtctggc gtcgttgcca ggtgtcgttc ccggcaagta
     8161 cgaccgcccg aacggctgcc tgcttaaccc gcgctgcccc tatgccactg acagatgtcg
     8221 cgctgaagaa ccggcgctga atatgctcgc tgacgggcgt cagtccaaat gccattaccc
     8281 acttgatgat gccgggaggc cgacactatg agtacgcaag aggccaccct gcaacaaccg
     8341 ctgttgcagg ctatcgacct gaaaaaacat tatccggtga agaaaggcat gttcgcgccg
     8401 gaacgtctgg ttaaagcgct ggatggcgtt tcgtttaacc ttgaacgtgg caaaacgctg
     8461 gcagtagtgg gcgaatctgg ctgcggtaaa tcgaccctcg gtcggttgct gacgatgatt
     8521 gaaacgccca ccggtggcga gctgtattac caggggcagg atctgcttaa gcacgatccg
     8581 caggcgcaga agctgcgtcg gcagaaaatc cagatcgtct tccagaaccc ttacggttcg
     8641 ctgaatccgc gtaaaaaagt cgggcaaatt cttgaagagc cgctgctgat caacaccagc
     8701 ttaagcaaag aacagcgtcg ggaaaaagcc ctgtcgatga tggcgaaagt cggcctgaaa
     8761 accgagcact atgaccgcta tccgcatatg ttctccggcg gtcagcgtca gcgtatcgcc
     8821 atcgcccgtg gtctgatgct cgacccggat gtggtgattg ccgatgaacc ggtttccgcg
     8881 ctggatgttt cagtgcgcgc gcaggtgctg aatctgatga tggatttgca gcaggagttg
     8941 gggctgtctt atgtctttat ctcccacgac ctgtcggtgg tggagcacat tgctgatgaa
     9001 gtgatggtga tgtacctggg ccgctgcgtg gagaagggaa cgaaagacca aatcttcaat
     9061 aacccgcgcc atccgtacac tcaggcgcta ctttccgcga cgccgcgcct gaacccggac
     9121 gatcgccgcg agcgcatcaa gctcagcggt gaactaccaa gcccactgaa tccaccgccg
     9181 ggttgcgcct tcaacgcccg ctgtcgtcgg cgcttcggcc cctgcaccca gttgcagccg
     9241 cagctaaaag actacggcgg tcaactggta gcttgttttg ctgttgatca ggatgaaaat
     9301 ccgcagcgtt aactcacaaa accggggcca tgcgctccgg tttactcaat cagcttcaac
     9361 agtggcgaca agcatagcaa aatgccgtaa agcagaatta gccaggcttt aaatccgcga
     9421 agtttttcca gttgtgcgac tttatagatc aggaaaaacg gaatgaggca cgacacaatg
     9481 ccatataacg ggcttcccaa ctgaaagaac accagcaccg atacacgaaa cgaaacccaa
     9541 atcgtcaacg taatgacgat aaaagcgcag atcgccagag tcagcatgcg tgagttaatt
     9601 ttcttggtat caataattcg gcttaacaga ttgagaataa tgcctttaat agcctcgtgg
     9661 aaaccgagat aaatgccaaa gaatgcggtc agtacggcaa agatattcag caccgtagag
     9721 gtgatatgaa tgatatgtcc agggatcacc tgcgcggcca gtgccagtgc cgagatattt
     9781 tgttcaaagg cagaaacggc ttcttcgtgg ctaattgaga aggtaaacga aaaggcaaaa
     9841 aacaggatca ccgcgatgag cgtgatataa ctaattcggt gggtacgcag cgcgagccgg
     9901 gttgccagta ctttatctgc ttcccgttta cgataggcaa tattcattgg gttaagtacc
     9961 tgaataaata ccgcagaaaa gaagcaaaat ggaattgtaa gcaggacatc gcggaaaaag
    10021 acggaggctt gcgggaaggc ggttatattg gcgaaattcc agtgcgggat catcgcaaaa
    10081 ccgaacacga caataatccc tactttgacc accaccattg gcccggaaat cttaaacagt
    10141 aatcgttcac caccagacgc aatcgctacc agtacggcga aaatagcgac tttataaagt
    10201 agagattgtg aaagatcggc atcggttaaa ccgaaggttt tcaggtacga ggcgctgtcg
    10261 aaaaccacgg agagagagta gataaaaatc ccgtggataa tcatcaaaaa gtagataacc
    10321 ccgaggaaaa ttccccagtt cttccccagg taatgactga taatatcggt gtagtcatta
    10381 caggaatcgc tttcagaaag ggtttttaaa taaatgtcct gcactaccca ggtggcagga
    10441 taagcaatga tcgctgcggt aataaatacc caaattccct tcaagccaat ttgtactggc
    10501 atcagcacgg ttccggcacc aatcgccatg cctatgcata ataaaaccca gccgaagtcg
    10561 tagcgtgtaa acggcaattt ctgattgtgt ttcgatagtg tgttgtgctg cataaagtcc
    10621 tgtccagaaa gaagtcggcc tcgcaaaaat gaataaaccc agtttctctt gcgcaacagg
    10681 tggagaagtc gaatacgcag tatgtaaaaa atgaggcaac agattcactg gggaggtcgc
    10741 gaggaggcgc aattctacaa aattgcgcat ttcactacca gtctggagtc agcccggata
    10801 tgaactgcaa ctattgcaaa taaagaaatt aagctggaag gcatcgcgca atgaggaaga
    10861 ttgccgggtg ctgacagtaa aaagcaatcg taaaatcagc ggcaagccga aagggttccg
    10921 gtgaggcgca atgttgcggg ggctttatcc ctggtggcat tggttgctgg agagagaaaa
    10981 cccccgcacg ttgcaggtat gcacctgaca acatcacggg ggctaatctt gaacccaaac
    11041 aactcaagaa tagccgcgaa acgttggcat tacaacacag gcggctatat gacgctcgca
    11101 gaactgggca tggccttctg gcatgattta gcggctccgg tcattgctgg cattcttgcc
    11161 agtatgatcg tgaactggct gaataagcgg aagtaatgtg tcatgcgggc gtcaggctgc
    11221 cgtaatggca atttgcgccc ggatcaggcc gcagaggtta aactctgcgg cctttttcgt
    11281 gcttactgcg ggtaaggcac ccagtcgccg ccgttcaggc gaacgtacgg tttatcctgg
    11341 tattgaataa ctactgcatt tgagttctcg gagaccggtg ctgtttgtgg caacccactg
    11401 gtgagttttt tccagtcaac attgtcttcg gtgaaaatct tgccatcaag aacgcgaacc
    11461 accagatcgg agatagccag gaagctgctc ggttggtcga tgacaatcgg tgccccctga
    11521 tgtggtgcct tcatgccgaa gaatttcacc ccaacgggga cgtctgtgat agacgggcta
    11581 gggatatcac gtaggccaga tacctgcatt ctgtcgccct tcagcgcgcc gccgtgttcc
    11641 ggcaccacga ccaccatcac tttacgaccc gatttctcca gttcagtaaa gaaggcgtcc
    11701 agttcatcaa agaatttctg cgcccgcgct ttgtaatccg ctgttttgct gacccccgga
    11761 taatggttgc cgtcatgcag tggaagcgtg ttgtagaacg tggcactacg gctgttttta
    11821 tctttttcgg taacgtccag ccagcggtta agcacggtgg tatcgtcata aaccggcgaa
    11881 ccatcaaagc ccagcaaaat aaccggcaga tttgtttgat ccatcaattc agtctgcatg
    11941 ccgccatttt cgcgaacttc tttcaaaaaa ccgccgaact ggccgttatg tcccatcatc
    12001 aggtgctggg taaagcccag tttcgaaagg ttatcaaaca gatagcagtc gttatttgcc
    12061 ggttgataca gattagtgtg cgaagtctgc ccgcagctgg cgcgcagtaa acggatcgcc
    12121 gccgggccac tgtaggaggt ggcggagtta aagttcttga actcaatatc gaaatgcgac
    12181 cacagtggat gcgacatcaa cccggcggct tctatatccg accaggaaag cgaacagatg
    12241 ttaatcacca gtagttcaaa tggctgagca tcagcgggca gcgaagacgg gaaggtcgat
    12301 ttacgtttcg cctccgcgtt atagaaatta ttcagccagg cgttaaggtt cgccgttgtt
    12361 ggcggtgtag tttgtgcggg catatcaccc actaccggtg cgccacccgt cgtctcaacg
    12421 gttgccgctg cgttaccacc cgtcgttgtt acagtggtcg tcggttgtcc ggctggccac
    12481 aaggagaaac ttggtcccgc cagggtaagt acgttcagcc atagcagtat ggcaaccaca
    12541 aaaacggtaa tgcgaatcca ttgtgacagg aataaccagg ccactaataa aacaaaaatg
    12601 gccccaatca tctgccagtt aataaagcgt gtgacaaggt cgattaaata atcggtactg
    12661 aaccccgcca cctgcgaacc ctggctcatt atgctttccg ggccaggcaa ccaggtgtca
    12721 tgccagaaca aagcaaagcc gatcggcagg gcaatccagt ggcgcaagcg atgcaggctg
    12781 tagcgcggaa tgggcatcag cagaaacgcg gcaaacacca aattgaggag cggatggaag
    12841 ttaagatatc ccgcccacaa caggccgaac ttaaccagaa aatagaagtt ccagccggaa
    12901 aggccgcgcc agtattgcca gagggaagaa ggcatggcgg tattttgcgt aaattgagtc
    12961 attttttggt tgccctggct ttttccgtgc ggcgtaatgt cccggccggt ttaacataac
    13021 gaggtttagc aaagaacaaa cgtaaggtgt cgcgaatgcg tcgcaaagag tgccgcgcca
    13081 gatagcccag cgggaaaaat atcagtgcgc aaacgacaat aatttcaatg atatcgctga
    13141 tggtcatcat gatgagcgct ccacagcatc atctaacagt cgcatgggtt ctggtattcg
    13201 tcgccagtgg cgaccatcgt gctcggcatt gatgaccggt ttagaacttt gcgttaaagg
    13261 cagcggcatg ccccattgtt ctggggcaag caggcgcatc tgcaccagct cggcactgat
    13321 ttgatcatct tcaaaccaga ccatacggtt tgagaaaatg tcgccagtag gcaatgggaa
    13381 aatatgattc aacgcggtat ccagatcgtt aatccgacag aatgagagaa acagcaccag
    13441 ccgattaccg ccaatggtca tgatatcgcc ggtacggtta gggcgacaca gcgtcagggc
    13501 ttgttcaacg cggatacccg gtaccggacg tagggcaacc agaacgcctt taccgtgggc
    13561 aggtaataga gggttattca tcatgttgtt gacggcatta cagaacacat cccacttctg
    13621 gaaaccacgc agtttgagcg gctgggtcat tgacagcaag gtagtgatat cttccggcac
    13681 atagcgacta aacttctgcc cttgcacgct ttcgatcatc gtcagacaac gggagagtgg
    13741 cgcattccac ggaataacca tatttgcacc gcaggccaat aacaaacgtt catcggtggc
    13801 gcgcaggcta gcggtatttt cccgcacgag gattttcatc gcactaccgc gctggcgacg
    13861 cagggtatga atgctgcggg ccagtggctc gatttgcgca ttttgctgta aagaaaagac
    13921 caccgtcgcc gcctgagcgg tacgggcttc attgaacagg acttcgttat tgttgaacag
    13981 ttgccagtgt tccgatagcg gcggcgcacc ttccagtaca gcaacattac tcagaatgcg
    14041 tttttcgtcg ctgcgtggtt ggatctccgc ctcttcgctt tgaactaatg tccagatacc
    14101 attttgttgc tgaacgctaa gctgctgacg ggcgctgacc cctttttcgt tgcaccagaa
    14161 ggcaatatcc agcaaatgtt ggtcaccctg aaaacgcaaa ctggcaagac caaaaagtga
    14221 acggtattcc tcaagcaaca atgaaaattg tttatcgtta ttatttccgg gattaattac
    14281 caaaagcgaa caatgggtta acctgctcca tttattcatt ttatccaacc atgagcgaag
    14341 ccgctcggca ggaatgtttt gccatgcgtt atttgcgcaa acaagaataa aaaggtaatt
    14401 atggggatca atagaacact gcaaatcacg ggtcaaatag tatagacctt tttcatgatt
    14461 tagcattgaa aataatttta ttttttccgg cccttgataa tcatctaatt gaaagatttt
    14521 cgccggatcg ctgtccatgc taatgaccgc gacgtgtgcg gtttcagcct gggatgcaat
    14581 tgtttgattc gccagactga tagcatcttc atggcgatcg acgttaaacc accagacgcc
    14641 gcctgctggc atatgtcgca gctcatccca taatgatgag ataccgatag agaatacagg
    14701 gcccacaatg tccctcatgt caccattttg tctgattatc agtttactag cgaaagctaa
    14761 gaaataaacc taccattgaa attaaaactt atcagattta gcatgtgaat caatttagaa
    14821 ggtcacaaga cgtgtgattt tagtcttttc gtttttaact ttttctgttg cgtaacggaa
    14881 agtcaaaaag tgagcaaatt cccgtctcgc cgtaaacaat aaaccggcga aatgctcacg
    14941 gttagaattg tctcatgaac ggtacggtta tttcataggg atcaagcaaa atgaataaca
    15001 atgaaccaga tactctgcct gatcccgcga taggctatat cttccagaat gatattgtgg
    15061 cgttaaagca ggcattttca ctgcctgata ttgattatgc cgatatttcc caacgcgaac
    15121 agttggccgc ggcattaaaa cgctggccgt tgctggcaga gtttgcgcaa caaaagtagg
    15181 ggattggtga atggccgtac tgggattgca gggggtgcgg ggaggcgtgg ggacaacaac
    15241 catcaccgcc gcattagcct ggtcattaca aatgttggga gaaaatgtcc tggtggtcga
    15301 tgcctgcccg gacaacttgt tgcgcctgtc atttaatgtt gattttaccc accgtcaggg
    15361 ctgggccaga gcgatgctgg atggccagga ctggcgtgac gctgggttgc gctacacctc
    15421 gcagctcgat ttgctgcctt ttggtcagtt atccattgaa gaacaagaaa atccacagca
    15481 caggcaaacc cggctgagcg atatttgctc cggcttacag caactaaaag ccagcgggcg
    15541 ttaccagtgg attttaatcg acttaccgcg tgatgcctcg cagataaccc accagctgct
    15601 gagtttgtgc gatcactcgc tggcaatcgt caatgtggat gccaactgcc atatccgact
    15661 gcatcagcaa gcgctgccgg atggcgcaca tattttgatt aatgacttcc gtattggcag
    15721 tcaggttcag gacgatattt accagctttg gttgcaaagc cagcgccgat tactgccgat
    15781 gctcattcat cgtgatgaag cgatagctga atgcctggcg gctaagcaac cagtaggtga
    15841 atatcgcagt gatgcgctgg cggctgaaga gatactgacg ctggcgaact ggtgcctgtt
    15901 gaactactcc gggctgaaaa cgccagtcgg gagtgcatca tgagtatcct gacccggtgg
    15961 ttgcttatcc cgccggtcaa cgcgcggctt atcgggcgtt atcgcgatta tcgtcgtcac
    16021 ggtgcgtcgg ctttcagcgc gacgctcggc tgtttctgga tgatcctggc ctggattttt
    16081 attccgctgg agcacccgcg ctggcagcgt attcgcgcag aacataaaaa cctgtatccg
    16141 catatcaacg cctcgcgtcc gctggacccg gtccgttatc tcattcaaac atgctggtta
    16201 ctgatcggta catcgcgcaa agaaacgccg aaaccgcgca ggcgggcatt ttcaggtctg
    16261 cagaatattc gtggacgtta ccatcaatgg atgaacgagc tgcctgagcg cgttagccat
    16321 aaaacacagc atcttgatga gaaaaaagag ctcggtcatt tgagtgccgg ggcgcggcgg
    16381 ttgatcctcg gtatcatcgt caccttctcg ctgattctgg cgttaatctg cgttactcaa
    16441 ccgtttaacc cgctggcgca gtttatcttc ctgatgctgc tgtggggtgt agcgctgatc
    16501 gtacggcgga tgccggggcg cttctcggcg ctaatgttga ttgtgctgtc gctgaccgtt
    16561 tcttgccgtt atatctggtg gcgttacacc tctacgctga actgggacga tccggtcagc
    16621 ctggtgtgcg ggcttattct gctcttcgct gaaacgtacg cgtggattgt gctggtgctc
    16681 ggctacttcc aggtcgtatg gccgctgaat cgtcagccgg tgccattgcc gaaagatatg
    16741 tcgctgtggc cgtcggtgga tatctttgtc ccgacttaca acgaagatct caacgtggtg
    16801 aaaaatacca tttacgcctc gctgggtatc gactggccga aagacaaact gaacatctgg
    16861 atcctcgatg acggcggcag ggaagagttt cgccagtttg cgcaaaacgt gggggtgaaa
    16921 tatatcgccc gcaccactca tgaacatgcg aaagcgggca acatcaacaa tgcgctgaaa
    16981 tatgccaaag gcgagttcgt atcgattttc gactgcgacc acgtaccaac gcgatcgttc
    17041 ctgcaaatga ccatgggctg gttcctgaaa gagaaacagc tggcgatgat gcagacgccg
    17101 caccacttct tctcgccgga cccgtttgaa cgcaacctgg ggcgttttcg taaaacgccg
    17161 aacgaaggca cgctgttcta tggtctggtg caggatggca acgatatgtg ggacgccact
    17221 ttcttctgcg gttcctgtgc agtaattcgt cgcaaaccat tggatgaaat tggcggtatt
    17281 gctgtcgaaa cggtaaccga agatgcgcac acgtctctgc gcctgcaccg tcgtggctac
    17341 acctctgcat atatgcgtat tccgcaggcg gcggggctgg cgaccgaaag tctgtcggcg
    17401 catatcggtc agcgtattcg ctgggcacgc gggatggtgc aaatcttccg tctcgataac
    17461 ccgcttaccg gtaaagggct gaaattcgcc cagcggctgt gctacgtcaa cgccatgttc
    17521 cacttcttgt cgggcattcc acgactgatc ttcctgactg cgccgctggc gttcctgctg
    17581 cttcatgcct acatcatcta tgcgccagcg ttgatgatcg ccctgttcgt gctgccgcat
    17641 atgatccatg ccagcctgac caactcgaag atccagggca aatatcgcca ctctttctgg
    17701 agtgaaatct acgaaacggt gctggcgtgg tatatcgcac caccgacgct ggtggcgctg
    17761 attaacccgc acaaaggcaa atttaacgtc accgccaaag gtggactggt ggaagaagag
    17821 tacgtcgact gggtgatctc gcggccctac atcttccttg ttctgctcaa cctggtgggt
    17881 gttgcggtcg gcatctggcg ctacttctat ggcccgccaa ccgagatgct caccgtggtc
    17941 gtcagtatgg tgtgggtgtt ctacaacctg attgttcttg gcggcgcagt tgcggtatcg
    18001 gtagaaagca aacaggtacg ccgatcgcac cgcgtggaga tgacgatgcc cgcggcaatt
    18061 gcccgcgaag atggtcacct cttttcgtgt accgttcagg atttctccga cggtggtttg
    18121 gggatcaaga tcaacggtca ggcgcagatt ctggaagggc agaaagtgaa tctgttgctt
    18181 aaacgcggtc agcaggaata cgtcttcccg acccaggtgg cgcgcgtgat gggtaatgaa
    18241 gttgggctga aattaatgcc gctcaccacc cagcaacata tcgattttgt gcagtgtacg
    18301 tttgcccgtg cggatacatg ggcgctctgg caggacagct acccggaaga taagccgctg
    18361 gaaagtctgc tggatattct gaagctcggc ttccgtggct accgccatct ggcggagttt
    18421 gcgccttctt cggtgaaggg catattccgt gtgctgactt ctctggtttc ctgggttgta
    18481 tcgtttattc tgcgccgccc ggagcggagc gaaacggcac aaccatcgga tcaggctttg
    18541 gctcaacaat gatgataacg cgatgaaaag aaaactattc tggatttgtg cagtggctat
    18601 ggggatgagt gcgttcccct ctttcatgac gcaggcgacg ccagcaacgc aaccactgat
    18661 caatgctgag ccagctgtag ccgcccagac ggaacaaaat ccgcaggtgg ggcaagtgat
    18721 gccgggcgtg cagggcgctg atgcgccagt cgtggcgcag aacggtcctt cgcgtgatgt
    18781 gaagctgacc tttgcgcaaa ttgcgcctcc gccgggcagc atggtgctac gtggcattaa
    18841 cccgaacggc agcattgagt ttggtatgcg cagcgatgaa gtggtgacga aggcgatgct
    18901 caacctcgaa tacatgccat cgccatcgtt actgcctgtc cagtcgcagt taaaggttta
    18961 tctcaatgat gagctgatgg gcgtactgcc agtgaccaaa gaacagttgg gtaaaaaaac
    19021 gctggcgcaa atgcccatta acccactgtt tattaccgac ttcaaccgtg tgcggctgga
    19081 gtttgtcggc cattatcagg acgtgtgcga aaacccggcc agcaccacgc tttggctgga
    19141 tgttgggcgg agcagtggac tggatctgac ctatcagacc ctgaatgtga agaatgacct
    19201 gtcacacttc ccggtgccat tctttgaccc gcgcgataac cgtactaaca ccttgccgat
    19261 ggtctttgcg ggtgcgccgg atgttgggct gcaacaagca tctgccattg tcgcctcgtg
    19321 gtttggttcg cgttctggct ggcgtgggca gaacttcccg gtgctctata accaactgcc
    19381 ggatcgtaat gccattgtct ttgcaaccaa cgacaaacgg ccggacttcc tgcgcgatca
    19441 tccggcggta aaagccccgg tgattgagat gatcaaccat ccgcagaatc cttacgtcaa
    19501 actgctggtg gtgtttggtc gtgacgacaa agacctgttg caggcagcga aaggtatcgc
    19561 tcagggtaac attctgttcc gtggtgaaag cgtggtagtg aatgaagtga aaccgctgct
    19621 accgcgtaag ccgtacgatg cgccgaactg ggtacgtacc gatcgtccgg tcacatttgg
    19681 cgaactgaaa acctatgaag aacagttaca atccagcggt cttgagccag cagcgattaa
    19741 cgtttcgcta aacctgccgc cggatctcta cctgatgcgc agtaccggca ttgatatgga
    19801 tattaattac cgctacacca tgccgccggt gaaagacagt tcgcggatgg atatcagcct
    19861 gaataaccag ttcctgcaat ccttcaacct gagcagcaaa caggaggcga accgcctgct
    19921 gctgcggatt ccggtattac aaggtttgct ggatggcaaa acagatgtct ctattccggc
    19981 gctgaaactg ggcgcgacca accagctgcg cttcgacttt gagtatatga acccgatgcc
    20041 gggcggttcg gtggataact gtattacctt ccagccggtg cagaatcatg tggtgattgg
    20101 tgacgactcc accatcgact tctcgaagta ttaccacttc atcccgatgc cggatctacg
    20161 cgcctttgct aacgcgggct tcccattcag ccggatggcg gatctgtcgc aaaccatcac
    20221 cgtgatgccg aaagcgccta acgaagcaca gatggaaacg ttgctgaata ctgttggttt
    20281 tatcggcgca cagacgggct tcccggcgat taatctgacg gtgaccgatg atggcagcac
    20341 cattcagggc aaagatgccg acatcatgat catcggtggt atcccggaca aactgaaaga
    20401 cgataagcag atcgacctat tggtgcaggc gaccgaaagc tgggtgaaaa caccgatgcg
    20461 ccagaccccg ttccccggca ttgtgccgga cgagagcgat cgcgcggcag aaacccggtc
    20521 aacgctgacc tcttccggtg cgatggcggc ggtgattggc ttccagtcgc cgtataacga
    20581 ccagcgcagc gtgattgcgc tgttggcaga tagcccacgc ggttatgaaa tgcttaacga
    20641 tgcggtgaac gatagcggca aacgcgccac catgttcggt tcggtcgcgg tgatccgcga
    20701 gtccggtatc aacagcctac gtgttggcga cgtttattac gtaggtcatc tgccgtggtt
    20761 cgagcgcgtg tggtatgcgc tggcaaacca tccgattctg ctggcggtgc tggcggctat
    20821 cagtgtgata ttgctggcat gggtactgtg gcgtctgctg cgaattatta gtcgtcgtcg
    20881 tcttaacccg gataacgagt aattgaagat gaatgtgttg cgtagtggaa tcgtgacgat
    20941 gctgttgttg gctgccttta gtgttcaggc agcctgtacc tggcctgcct gggagcagtt
    21001 taaaaaggat tacatcagtc aggaagggcg cgtcatcgac cccagcgacg cgcgcaaaat
    21061 caccacctcc gaagggcaaa gttacggtat gttctttgcc ctggcggcta acgaccgtgc
    21121 agctttcgat aatattctcg actggacgca gaacaatctc gctcagggtt ctttaaaaga
    21181 acgtttgccc gcctggctgt ggggcaagaa agagaacagt aagtgggaag tgctggacag
    21241 caattcggcc tccgatggtg atgtctggat ggcctggtcg ttgctggagg cggggcgttt
    21301 gtggaaagag cagcgttata ccgacatcgg cagcgcgttg ctaaaacgta tcgcgcggga
    21361 ggaagtggtg acggtgcctg ggctgggttc catgttgtta ccgggcaaag tgggttttgc
    21421 tgaggataac agctggcgtt ttaaccccag ctacctgccg ccgacgctgg cgcagtattt
    21481 cacccgcttt ggcgcgccgt ggactacgct gcgcgaaacc aatcaacgtt tattgctgga
    21541 aaccgccccg aaaggctttt cgccagactg ggtgcgttat gagaaagaca aaggctggca
    21601 gctaaaagcc gaaaaaacat tgatcagcag ctacgacgcc atccgcgttt acatgtgggt
    21661 aggcatgatg cctgacagcg atccgcagaa agcgcggatg ctcaaccggt ttaaaccgat
    21721 ggcgacattc actgagaaaa acggttatcc gccggaaaaa gtggatgtgg ctacggggaa
    21781 agcgcagggt aaaggaccag ttggtttttc tgccgccatg ctgccctttt tacaaaaccg
    21841 cgatgcgcag gccgttcagc gtcagcgcgt ggccgataac tttcccggca gcgatgccta
    21901 ttacaactat gtgctgaccc tgtttggaca aggctgggat caacaccgtt tccgcttctc
    21961 gacaaaaggt gagttattac ctgactgggg ccaggaatgc gcaaattcac actaaacata
    22021 ttcacgcttt ccctcggtct ggccgtcatg ccgatggtcg aggcagcacc aaccgctcag
    22081 caacagttgc tggagcaagt tcggttaggc gaagcgaccc atcgtgaaga tctggtgcaa
    22141 cagtcgttat atcggctgga acttattgat ccgaataacc cggacgtcgt tgccgcccgt
    22201 ttccgttctt tgttacgtta gggcgatatt gatggcgcgc aaaaacagct cgatcggctg
    22261 tcgcagttag cgccgagttc aaatgcgtat aaatcgtcgc ggactacgat gctactttcc
    22321 acgccggatg gtcgtcaggc actgcaacag gcacgattgc aggcgacgac cggtcatgca
    22381 gaagaagctg tggcgagtta caacaaactg ttcaacggtg cgccgccgga aggtgacatt
    22441 gctgtcgagt actggagtac ggtggcgaaa attccggctc gccgtggcga agcgattaat
    22501 cagttaaaac gcatcaatgc ggatgcaccg ggcaatacgg gcctgcaaaa caatctggcg
    22561 ctattgctgt ttagtagcga tcgccgtgac gaaggttttg ccgtcctgga acagatggca
    22621 aaatcgaacg ccgggcgcga aggggcctct aaaatctggt acgggcagat taaagacatg
    22681 cccgtcagtg atgccagtgt gtcggcgctg aaaaaatatc tctcgatctt tagtgatggc
    22741 gatagcgtgg cggctgcgca atcgcaactg gcagaacagc aaaaacagct ggccgatcct
    22801 gctttccgcg ctcgtgcgca aggtttagcg gcggtggact ctggtatggc gggtaaagcc
    22861 attcccgaac tacaacaggc ggtgcgggcg aacccgaaag acagtgaagc tctgggggcg
    22921 ctgggccagg cgtattctca gaaaggcgat cgcgccaatg cagtggcgaa tctggaaaaa
    22981 gccctcgcac tggacccgca cagcagcaac aacgacaaat ggaacagtct gctgaaagta
    23041 aaccgctact ggctggcgat ccagcagggc gatgctgcgc tgaaagccaa taatcctgac
    23101 cgggcagaac gcctgttcca gcaggcgcgt aatgtcgata acaccgacag ttatgcagtg
    23161 ctggggctgg gcgatgtggc gatggcgcga aaagattatc ccgccgccga acgttattat
    23221 cagcagacct tgcgtatgga cagcggcaac actaacgccg tgcgcgggct ggcaaatatt
    23281 taccgccagc aatcgccaga aaaagctgaa gcgtttatcg cctcgctctc tgccagtcag
    23341 cggcgtagca ttgatgatat cgaacgcagc ctgcaaaacg accgtctggc acagcaggca
    23401 gaggcactgg aaaaccaggg caaatgggcg ccggcggcag cacttcagcg gcaacgactg
    23461 gcgctggacc ccggcagcgt atggattact taccgacttt cgcaggatct ctggcaggcc
    23521 ggacaacgca gccaggccga tacgttaatg cgcaatctgg cgcagcagaa gtcgaacgac
    23581 ccggagcagg tttacgctta cgggctgtac ctctctggtc atgaccagga cagagcggcg
    23641 ctggcgcata tcaacagcct gccgcgcgcg cagtggaaca gcaatattca ggagctggtt
    23701 aatcgactgc aaagcgatca gatgctggaa accgctaatc gcctgcgaga aagcggcaaa
    23761 gaagctgaag cggaagcgat gctgcgtcag caaccacctt ccacgcgtat tgacctcacg
    23821 ctggctgact gggcgcagca gcgacgtgat tacaccgccg cccgcgctgc atatcagaat
    23881 gtcctgacgc tggagccaac taacgccgat gccattctgg ggctgacgga agtggatatt
    23941 gctgccggtg acacagcggc ggcacgtagc cagctggcga aactgcccgc taccgataac
    24001 gcctcgctga acacacagcg gcgcgtggcg ctggcgcagg cgcagcttgg agataccgca
    24061 gcggcgcagc agacgtttaa taagttgatc ccgcaggcaa aatctcagcc accgtcgatg
    24121 gaaagcgcga tggtgctgcg cgacggtgcg aagtttgaag cgcaggcggg cgatccaaag
    24181 caggcgctgg aaacctacaa agacgccatg gtcgcatccg gtgtgactac gacgcgtccg
    24241 caggataacg acacattcac ccgactgacc cgtaacgacg agaaagatga ctggctgaaa
    24301 cgcggcgtgc gcagcgatgc ggcgggcctc tatcgccagc aggatcttaa cgtcaccctc
    24361 gagcacgatt actggggttc gagcggcacc ggtggttact ccgatctgaa agcgcacact
    24421 accatgttgc aggtggatgc gccgtattct gacgggcgga tgttctttcg cagtgatttc
    24481 gtcaatatga acgtcggcag tttctccact aatgccgatg gcaaatggga tgacaactgg
    24541 ggcacctgta cattacagga ctgtagtggc aaccgcagcc agtcggactc cggtgccagc
    24601 gtggaggtcg gctggcgaaa tgacgtctgg agctgggata tcggtaccac gccgatgggc
    24661 ttcaacgtgg tggatgtggt cggcggcatc agttacagcg atgatatcgg gccgctgggt
    24721 tacaccgtta acgcccaccg tcggcccatc tccagttctt tgctggcctt tggtgggcaa
    24781 aaagactccc cgagcaatac cgggaaaaaa tggggtggcg tacgtgccga cggtgtgggg
    24841 ctaagtctga gctacgataa aggtgaagca aacggcgtct gggcatcgct tagtggcgac
    24901 cagttaaccg gtaaaaatgt cgaagataac tggcgcgtgc gctggatgac gggctattac
    24961 tataaggtca ttaaccagaa caatcgccgc gtcacaatcg gcctgaacaa catgatctgg
    25021 cattacgaca aagatctgag tggctactca ctcggtcagg gcggttacta cagtccgcag
    25081 gaatacctgt cgtttgctat accggtgatg tggcgggagc gcacggaaaa ctggtcgtgg
    25141 gagctggggg cgtctggctc gtggtcgcat tcacgcacca aaaccatgcc gcgttatccg
    25201 ctgatgaatc tgatcccgac cgactggcag gaagaagctg cgcggcaatc caacgatggc
    25261 ggcagcagtc agggcttcgg ctacacggcg cgggcattac ttgaacgacg tgttacttcc
    25321 aactggtttg ttggcacggc aattgatatc cagcaggcga aagattacgc acccagccat
    25381 ttcctgctct acgtacgtta ttccgccgcc ggatggcagg gtgacatgga tttaccgccg
    25441 cagccgctga taccttacgc cgactggtaa gttttcagat agcgcctctc ttaatgccgc
    25501 tgcgatcggg tatactcggg cggcaatctg ggatttccgg ggggagacaa tttgcgcgta
    25561 agtcgctcgt taacaatcaa gcagatggca atggtggcag ccgttgtcct ggtgttcgtt
    25621 tttatttttt gcaccgtttt gctgttccat ctggtccagc agaatcgcta taacacggct
    25681 acgcaactgg aaagcattgc tcgctctgta cgcgaaccct tatcttcagc tattttgaaa
    25741 ggcgatattc ccgaagcgga agctattctt gccagcatta aaccggcagg cgtggtcagc
    25801 cgtgccgatg tagtgctgcc taaccagttc caggcgctgc gtaaaagttt tattccagag
    25861 cgcccggtgc cggtaatggt tactcgcctg tttgagctac cggttcaaat ctcgctgggc
    25921 gtttactcgc tcgaacgtcc ggcaaacccg cagccaattg cctatctggt actacaggcg
    25981 gattccttcc gtatgtataa gttcgtgatg agcaccctct caacgttagt gaccatttac
    26041 ttacttttgt cgcttatcct gaccgtcgcc atcagctggt gcattaaccg cctgattttg
    26101 catccgttac gcaatattgc tcgcgaactt aacgccatcc cagcccagga gcttgttggt
    26161 caccaactgg cattaccgcg tctgcatcag gacgatgaaa tcggtatgtt ggtgcgcagt
    26221 tacaacctca accagcaatt gctgcagcgc cattatgaag aacagaacga aaatgcgatg
    26281 cgcttcccgg tgtcggattt gccgaacaaa gccttgctga tggagatgct ggagcaggtt
    26341 gtcgcgcgta aacaaaccac cgcgctgatg atcatcacct gtgaaaccct gcgtgatact
    26401 gcgggcgtgc tgaaagaggc gcaacgagaa attctgctgc tgacgctggt ggaaaaactc
    26461 aaatcggtac tgtcgccacg tatgatcctc gcgcagatta gcggttatga ctttgctgtc
    26521 attgccaacg gtgtacagga accgtggcac gcaatcacct taggtcagca agtgctcact
    26581 atcatgagcg agcgcctgcc gattgaacgt attcaactcc gtccgcactg tagcattggc
    26641 gtggcgatgt tctacggcga tctcaccgcc gaacagcttt acagtcgcgc tatttctgcg
    26701 gcatttaccg ctcgccataa aggcaagaat cagattcagt tctttgatcc gcagcagatg
    26761 gaagccgccc agaagcggtt gacggaagag agcgatatcc ttaatgcact ggaaaatcat
    26821 cagtttgcta tttggttaca gccacaggtc gagatgacca gcggtaaact ggtcagtgcg
    26881 gaagtgttac tgcgtatcca gcaaccggat ggcagttggg acctgccgga tggcttaatc
    26941 gatcgcattg agtgctgtgg gctgatggtt accgtcggtc actgggtgct ggaagagtcc
    27001 tgtcgattgc ttgcagcctg gcaagagcgc ggcattatgc tgcccttgtc ggtaaacctc
    27061 tctgcgctgc aactgatgca cccaaatatg gtggcggata tgctggaact gttaacccgc
    27121 tatcgcattc aaccgggaac actgattctg gaagtgacag aaagccgacg tattgacgac
    27181 cctcatgctg cggtggcaat cctccgtccg ctgcgcaatg ccggagttcg ggtggcgctg
    27241 gatgatttcg gcatgggcta cgcagggctg cgtcagctgc agcatatgaa atcgttgcca
    27301 atcgacgtac tgaaaatcga caaaatgttt gttgaaggct tgccggaaga tagcagcatg
    27361 attgctgcaa ttatcatgct ggcgcagagc ctgaacttac aaatgattgc cgaaggcgtg
    27421 gagactgaag cacaacgcga ctggctggca aaagcgggcg ttggtattgc ccagggcttc
    27481 ctttttgctc gcccactccc tattgaaatc ttcgaagaga gttacctgga agaaaagtag
    27541 ctaccccaaa ctgattacaa aactttaaaa agtgctggtt tgtgcgagcc agctcaaact
    27601 ttttaacctt tttgtttcaa ttatgatcca ggtacatttc tgtgatgttg tctgggtgtt
    27661 attttaaggc cgcaggtacc tcataacctt acaagacctg tagttttact aaaggacacc
    27721 ctatgaaaac ctctctgttt aaaagccttt actttcaggt cctgacagcg atagccattg
    27781 gtattctcct tggccatttc tatcctgaaa taggcgagca aatgaaaccg cttggcgacg
    27841 gcttcgttaa gctcattaag atgatcatcg ctcctgtcat cttttgtacc gtcgtaacgg
    27901 gcattgcggg catggaaagc atgaaggcgg tcggtcgtac cggcgcagtc gcactgcttt
    27961 actttgaaat tgtcagtacc atcgcgctga ttattggtct tatcatcgtt aacgtcgtgc
    28021 agcctggtgc cggaatgaac gtcgatccgg caacgcttga tgcgaaagcg gtagcggttt
    28081 acgccgatca ggcgaaagac cagggcattg tcgccttcat tatggatgtc atcccggcga
    28141 gcgtcattgg cgcatttgcc agcggtaaca ttctgcaggt gctgctgttt gccgtactgt
    28201 ttggttttgc gctccaccgt ctgggcagca aaggccaact gatttttaac gtgatcgaaa
    28261 gtttctcgca ggtcatcttc ggcatcatca atatgatcat gcgtctggca cctattggtg
    28321 cgttcggggc aatggcgttt accatcggta aatacggcgt cggcacactg gtgcaactgg
    28381 ggcagctgat tatctgtttc tatattacct gtatcctgtt tgtggtgctg gtattgggtt
    28441 caatcgctaa agcgactggt ttcagtatct tcaaatttat ccgctacatc cgtgaagaac
    28501 tgctgattgt actggggact tcatcttccg agtcggcgct gccgcgtatg ctcgacaaga
    28561 tggagaaact cggctgccgc aaatcggtgg tggggctggt catcccgaca ggctactcgt
    28621 ttaaccttga tggcacatcg atatacctga caatggcggc ggtgtttatc gcccaggcca
    28681 ctaacagtca gatggatatc gtccaccaaa tcacgctgtt aatcgtgttg ctgctttctt
    28741 ctaaaggggc ggcaggggta acgggtagtg gctttatcgt gctggcggcg acgctctctg
    28801 cggtgggcca tttgccggta gcgggtctgg cgctgatcct cggtatcgac cgctttatgt
    28861 cagaagctcg tgcgctgact aacctggtcg gtaacggcgt agcgaccatt gtcgttgcta
    28921 agtgggtgaa agaactggac cacaaaaaac tggacgatgt gctgaataat cgtgcgccgg
    28981 atggcaaaac gcacgaatta tcctcttaat ctcaccactt atgcccgcac tcccttcccc
    29041 gagtgcgggt gtttgcgcat aattgacccc tgtgcctgaa cttaccacct gggtgagttc
    29101 aggtttattt cacttcttcc gtgacatttt catgttcttg cggtctaaca cgaagtgttt
    29161 ttaacgtcat ttagactgac tgacaacggg cagtttttta ttaccaggat agttgatcag
    29221 gggttcacat gcagggcaca aaaattcgac ttttagcggg cggtttgctg atgatggcca
    29281 ctgctggcta tgtgcaggca gatgcgctcc agcctgatcc agcatggcaa caggggacgc
    29341 tttccaacgg tttacagtgg caagtgctga ccacccccca gcgtcccagc gatcgtgttg
    29401 aaattcgcct gctggttaat accggttcgc tcgccgaaag tacacaacag agcggttaca
    29461 gtcacgccat ccctcgtatt gcgctaacgc aaagcggtgg ccttgacgca gcacaggcgc
    29521 gttcattgtg gcagcagggg atcgacccta aacgcccgat gccgccggta attgtctctt
    29581 atgacaccac gctgtttaat ctgagtttgc ccaataaccg taacgatttg ctgaaagaag
    29641 cgctctctta tctggcaaat gccactggca aattgaccat cacaccagaa accatcaacc
    29701 acgcgctgca aagtcaggac atggtggcaa cctggcctgc cgatactaaa gagggctggt
    29761 ggcgctatcg tctgaaaggg tcaaccttgt taggtcacga tcctgccgat ccgctgaaac
    29821 aacccgttga agcggaaaaa attaaagatt tctatcagaa atggtacacc ccggatgcaa
    29881 tgacgctact ggtggtggga aacgtggatg cgcgctcggt tgtcgaccaa atcaacaaaa
    29941 cgtttggcga actgaaaggc aaacgtgaaa cgccagctcc ggtgccgacg ctttctccgc
    30001 tgcgtgcgga agcggtgagt attatgactg acgcggtgcg tcaggaccgg ttatctatca
    30061 tgtgggatac gccgtggcag ccgattcgtg aatcagccgc actgctgcgc tactggcgtg
    30121 cggacctggc ccgcgaggcg ctgttctggc atgttcagca agcgttaagc gccagtaaca
    30181 gcaaagacat cggtcttgga tttgactgcc gtgtgctgta tctgcgtgcg cagtgtgcca
    30241 tcaacatcga atcaccaaac gacaagctga acagcaacct taatctggtg gcgcgtgaac
    30301 tggcgaaggt tcgcgataaa ggtctgccgg aagaagagtt caatgcgtta gtggcgcaaa
    30361 agaaactgga gctgcagaaa ctgtttgccg cctatgcgcg ggctgatacc gatattctga
    30421 tgggtcagcg gatgcgttcg ttgcaaaatc aggtcgtcga tatcgcgccg gagcagtatc
    30481 agaaactgcg gcaggatttc cttaacagcc tgacggtgga gatgttaaat caggatctgc
    30541 gtcagcagtt gtcgaatgat atggcgttaa tactgctgca gccgaaaggc gagccggaat
    30601 ttaacatgaa agcgttgcag gcggcctggg atcaaatcat ggccccatcg actgctgctg
    30661 ccgccacctc tgtcgccacg gatgacgtac atcctgaagt gacggatatt ccacctgcac
    30721 agtaatgcct gatggtgatg agttagcaca accatcaggt atttatggat gagctggata
    30781 gaggggtaac actttatcca gccttttgca tatgctgcgt ttacgctggc atcgcctcac
    30841 gcgggataat cgcgccgcga tactgaataa cggtacttgc ggtcaggtgc ccgcgtttcg
    30901 ccgcgttttc cgcactgccg cccgtcaaac gtaccgccag ataaccggcg ctgaaagagt
    30961 cgccagctgc ggtggtatcg atcacttttt ctttcggcag tttcaccgcc ggaacatcca
    31021 ctaacccttc gccagcaatg gacaccaggc aagaatccgc cccgcgtttc accaccactt
    31081 ctttcacgcc cgcgttatgg gtgcgcgcaa tgacgtcttc caccggctgt tgaccccaca
    31141 gcgcgtcttc atcgtccagc gtcaggaagg cgatatccgt gcattccagc atttgttggt
    31201 acacctgctg tgtctcttct ttgctggccc acaggcgcgg acgatagtta ttgtcgaaaa
    31261 tcacttttcc gccgttggcg cggcattcgc gcagcaggga aagcagcttt tcgcggctgg
    31321 tcgggcttaa gatcgccagg ctaatcccgc tcaggtagag ataatcgaaa ttcgccagct
    31381 cttcgcaaat cgccgtagac tgctcactct ccagccagaa tttggcggcg gcttcgttcc
    31441 gccagtagta gaacgtacgc tcgccggtgc tgtcggtttc aatgtagtaa aggcccggca
    31501 gacggttttc catccgttgg gtcagggaaa tatcaacgtt ctcgccgtgc caggcgtcca
    31561 gcatctgctg gctaaaactg tccgttccca gcgccgttac gtaatgaacg gttaatgccg
    31621 caggatcgac ctgacgggcg atatagacgg aagtgttcag ggtatcgccg ccgaaaccgc
    31681 gcttaacgtc cgcgcctttc tcggaaagct caatcatgca ttcgccaatc acggcaatct
    31741 ttttggacat agtcgtgaac ctgatctgta aaaaattagc gttagtttgc gctgtggtgg
    31801 tttgctggtc aacaatatta aaacagtgtt ccattatttt tttgagccag aacgtattcc
    31861 tgaaagattc cgttgtggag tgaggaaatt taccccggcc aggcggtaaa gttctgccct
    31921 tacgcgccga taatctttgt cgagtccggg cagcatcact tttaaacaca ggacatcttt
    31981 gatgataagg caggttatcc agcgaataag caaccctgaa gcaagcatcg agagcttgca
    32041 ggaacggcgt ttttggttgc agtgtgagcg tgcttacacc tggcagccga tctatcaaac
    32101 atgcgggcgg ttaatggccg tggagctatt aacggtggtc acgcatccct tgaacccttc
    32161 gcaacgcctg ccgccggatc gctattttac tgaaatcacc gtcagccatc ggatggaggt
    32221 tgtgaaagag cagattgatt tgctggcgca aaaagccgac ttctttatag agcacggcct
    32281 gctggcatcg gtcaatattg atggccctac gctcatcgcc ctgcgtcagc aaccaaaaat
    32341 cctgcgccaa attgagcgtc ttccctggct gcgtttcgaa ctggtggagc atatccgtct
    32401 gccgaaagat tcaacctttg cctcgatgtg tgaatttggc ccgctgtggc tgggtgattt
    32461 tggtaccggg atggcaaatt tctctgcact aagtgaagtg cgttatgact acatcaaaat
    32521 cgcgcgagaa ctgtttgtga tgctgcgtca gtcgccggaa ggacgcacac tcttttctca
    32581 gcttttacat ctaatgaatc gctattgtcg cggggtgatt gttgagggcg tagaaacgcc
    32641 ggaagagtgg cgtgatgtac agaactcgcc cgcattcgcc gcacaaggct ggtttctttc
    32701 acgcccggca ccgatagaaa cgctgaatac ggcggttctg gcgctataag ctgcctcatt
    32761 ttccgcctgg ctggactatc tttaggactg gcacaggaag aatatgagga agcgaacgat
    32821 gagcaaggca ggcaaaataa ctgctgcgat ttcaggggct ttcttgttgt tgattgtcgt
    32881 ggcgatcatt ttgattgcaa cttttgactg gaatcgactc aaaccgacca tcaaccagaa
    32941 agtctctgcg gagttgaatc gtccgttcgc tatccgtggc gatctgggcg tggtgtggga
    33001 gcggcaaaaa caagaaactg gctggcgcag ctgggtgccg tggcctcatg tacatgcaga
    33061 agacatcatt cttggcaatc caccggatat tcccgaagtc acgatggtgc atttgccgcg
    33121 agtagaggca acgctggcac cgctggcgct gctgaccaaa acggtctggc tgccgtggat
    33181 caagctcgaa aagcccgacg cgcgcctgat tcgcctctcc gaaaagaaca ataactggac
    33241 gtttaatctc gccaacgatg ataacaaaga cgcgaatgca aagccgtcgg catggtcgtt
    33301 tcggctggat aatattcttt tcgatcaagg gcggatcgcc attgatgaca aagtaagcaa
    33361 agcggatctg gaaatttttg ttgatccgtt aggcaagccg ctgccgttca gcgaagttac
    33421 tggatcgaaa ggtaaagcgg ataaagaaaa ggtgggcgat tacgtttttg gcctgaaggc
    33481 gcagggacgt tataacggtg aaccgctcac gggtacggga aaaataggcg gtatgctggc
    33541 gctgcgtggc gaagggacgc cgtttccggt acaggctgat ttccgttcag gtaatacccg
    33601 tgttgctttt gatggcgtcg tgaatgaccc aatgaagatg ggcggtgtcg atttacggct
    33661 taaattttct ggcgattcac tgggtgatct ctatgaactg acgggcgttc tgctgcccga
    33721 taccccgccg tttgaaacgg atggtcggct ggtagcgaaa atcgacactg aaaaatcgtc
    33781 ggtctttgat tatcgcggtt ttaatgggcg aattggcgat agcgatatcc acggttctct
    33841 ggtctacacc accggaaagc cgcgaccaaa actggaaggt gatgtcgagt cgcggcaatt
    33901 gcggctggcg gacctgggac cgttgattgg cgttgattcg gggaaagggg cagaaaagtc
    33961 gaaacggtct gaacagaaga agggcgaaaa aagcgttcag cctgcgggca aagtgctgcc
    34021 ttatgaccgc ttcgaaaccg ataaatggga cgttatggat gccgatgttc gcttcaaagg
    34081 ccgacgtatc gagcatggca gtagcctgcc gattagcgat ctttctactc atatcatcct
    34141 caaaaatgct gacctgcgcc tgcaaccgct gaaatttggc atggcgggcg gcagcattgc
    34201 ggcgaatatc catctggaag gcgataaaaa gccgatgcag gggcgggcag atattcaggc
    34261 tcgtcgactg aaactgaaag aactgatgcc cgatgtggaa ctgatgcaga agacgctggg
    34321 ggaaatgaac ggtgacgcgg aactacgcgg tagcggtaac tcggtggcgg cgcttttagg
    34381 caacagtaac ggcaacctga aactgttgat gaatgacggg ctggtgagcc gcaacctgat
    34441 ggagattgtt gggctgaatg tcggcaacta cattgtcggt gcgatatttg gtgacgatga
    34501 ggtgcgggtg aactgcgcgg cggcgaatct gaatattgcc aacggcgtag cgcgcccgca
    34561 gatttttgct ttcgatactg agaacgcgtt gattaatgtt accggcacgg caagttttgc
    34621 ttcggaacag ctggatttga ctattgatcc ggagagtaaa gggattcgga ttatcacact
    34681 gcgttcgccg ctgtatgtgc gtgggacgtt taaaaatccg caggctgggg tgaaagccgg
    34741 accgctgatt gcccgtggtg ccgttgcggc ggcactggca acgctggtaa caccggcggc
    34801 ggcgttattg gcactgatct caccttccga aggggaggct aatcagtgtc ggacgatatt
    34861 gtcgcagatg aagaagtgat gaggttggat tgtgtttgct ggtgagttcg ggagcagcgt
    34921 gttcccctca ccctaatcct ctccccaaag gggcgaggag actgttttgt gcgtgttgaa
    34981 acaacgagta ccgcttttcc gttcactttc tgggggaggg ctgttatgtt cgcgatataa
    35041 catctcgctc cgcttttctc cctctccctt tcagggagag ggctggggtg agggtggctg
    35101 cccacacgat acttacaacg actgatgttg cgtctcatgg gtcagcagca gggcgattaa
    35161 cgtcaagcca gccatcgccg ccagatataa ccccaccgca cctaacccgt agttagtctg
    35221 caaccaggct gcgatatacg gcgcaacgga agccccgaga atcgacgata cgttgtaaga
    35281 gaacgatgct ccggtgtaac gcacttctgt cgggaacagc tctggtaaca gcgcgcccat
    35341 cggaccgaag gtcagcccca tcagacttaa ccccagcagc aggaaggcaa aaaccagaat
    35401 cgggttgcca gaaccgagca aagggttaaa ggcgaatagc gcgaacagga tgagcagcgt
    35461 ggtgatgatt accatgcttt tacggcgacc aaaggcatca gccagtaatc cagcgactgg
    35521 caccatcacg ccaaaaccaa taactgccat catcaacatc cacaacactt cgttacgcgg
    35581 caggccaagc ccaactggcg cggcggcggt actaaaggtc atggagtaga ccgtcatgat
    35641 gtaaaacagc gtataggttg ccagcataat gaacgtaccc agtacggtta cgcgaacatg
    35701 tttggtcagc agcgtaccca gcgggatctt cacctgtttt ttcgctttag cgactttctc
    35761 aaacaccggc gactcatgca gcgacacgcg aacatacagg ccgataatga ccagcaccgc
    35821 cgagaagata aatggcacac gccagcccca gctcataaac tgctcgtcgg tcagtagcca
    35881 ggaaagcagc aagaaagtgc cgttggcaaa gaagaagcca atcggtgcgc ccagctgcgg
    35941 gaaggagccg tacaatgcac gtttgcgcgg tggggcgttt tcagtcgcca gcagcgccgc
    36001 gccgccccat tcaccaccta agcccagacc ctgaccaaat cgagccagcg ccagcagcag
    36061 cggggcgaaa ataccaatcg tggcatagcc tggcagcaga ccaatcacca cggtcgaaat
    36121 ccccatcgtt agcaacgagg cgaccagcgt cgctttacgc ccaacgcgat cgccaaaatg
    36181 accaaaaacg gcagagccaa tagggcgcgc gacgaaggcg atggcgaagg tggcgagcga
    36241 ctgtagcgtt gctgccgtag gatcgccctg cgggaagaag atatgcggaa acacaataac
    36301 ggccgcagtg gcgtaaatgt aaaagtcgaa gaactcaatg gctgtgccaa tgagagaggc
    36361 gacaaggact ttattacgcg agttgatcgg cgtgtattct tgctcgtggt cgagtgttgt
    36421 ggctgttgct tgcataatct tttcttattt tttggctaac gaatagccat attacgcaca
    36481 gcaaaagcga tatttcaatg tgtaacaaat cgcggaaatg gtcaaaaacc aggcaaaaag
    36541 gggataatgt ttaggccagt gatttttctt ctatgaaatg cttcacataa ttttataaat
    36601 agtggataaa actggtttca ggttaaataa aagttaccaa ctggatttat atgctggggt
    36661 tatgagttag aatgaaattg tttgctccca aatagatggc tgaagaggct ctggggacga
    36721 gtttcaagag gttatgtgcg cattatagcc ggatgcgatg ctggcgcatc ttatccggcc
    36781 tacggggagc caggccgaat aaaatcgtat gccggataag gcgtttacgc cgcatccggc
    36841 ctattttaag gctgcgtttt ccctggcatt cgcgggtcgt ctttatattc ggcggtggca
    36901 atccacgccg cgcaaaacag cgtcaaacgg gcgaagaagt agaaaaatgc catcagcccc
    36961 agcacggaac caaatgctgc gccagagggg gatttcatca gcgatggcag ggtgtaggtc
    37021 atcacgattt taatcacttc aaaaccaata gcagccagaa atgttccgcg aatcagggct
    37081 tttttgcgcg gacggtggcg cggcaaccgc cagaagatcc agaagaaaag cagatagttg
    37141 gcgaagatgg aaatcgccag gccaatcaat cgccacgtcg gtttcagcca ctcaatgctg
    37201 ttcaggtgca gggcgctaat aatcatttgc tgcgccgaac cggcaaccga ggtgatcgaa
    37261 agcgtcacaa tcagcgcaat caacaaacca atcagtgaaa taaaatcacg cagatatttt
    37321 acccagaact tctcctgatc ttgcggcgag cgttcccaga catcacgcga ctgggcacga
    37381 atcgcttcac gcaggttacc catccagttg atgccggaat aaagtgccac cgccaggccg
    37441 acaagcccta cagtcgtacg ctgctgaacg gcggtgttga tggtgttttt caacgtggcg
    37501 gctagtgtcg gatcgctgat gttttgcaga attttgtcga agatatcctg tagcaacatc
    37561 ggatgggagg ccagcacaaa gccccctgcg gcaaacgaca ccatcaaaat cgggatcatc
    37621 gacaaaaatg agaaataggt gatagccgca ccaaactggt tacccagccg atcgttaaag
    37681 cgttctgtcg cgcgaatcag gtgcgcaata accggttggc gttggacttt ttcggcagtg
    37741 gtggtgacgg tttccagcgc ctggctaact ttgccgcctt tctcgctatt atccaactgc
    37801 ttaatcggct cgtgctccag atcctgggtg ggacgtttga tctcgttttc ctgcgtcatc
    37861 cgctcttttg tccttctgtc gtggtcattc ttaaaaagta tagtcggtca gtccaggtgc
    37921 tctttcatta cgccagccag ccactgcata aacaggttta cacgccggga aagctcccga
    37981 cgctgcgggt aaaccagcga aagggagagc ggctcggcac ggtagccagg taatacttca
    38041 ataagccgcc cggcacgcag ggcttcgcgc acggcaatgc gcgggatctg aataatcccc
    38101 agaccggcaa gaccggcggc gagatagttt tcgctactat ttaccgtcaa catgccgcca
    38161 gacttaaacc actggacgcc attaacgctg gcaacctcaa aacctaacgg atgtacaccc
    38221 aggtgcggtg tataacgcac tattgcgtgt gaagtcagat catcggggct ttgcggataa
    38281 ccaaagcgcg tcagatagtg cggactggca cagttgacca tggtcagttt gccgagggga
    38341 cgggcgataa cgccatcttc cggtaacgcg ccagtgcgta tcacgcaatc aaaaccatca
    38401 tgaagaatat ctaccggacg gtcatggcta ctcagttcca gctcaattcc cggatgcaga
    38461 tagagaaatt ccgacaggcg cggcagtaac aggtttttcg cgattcctgg cgggatgtcg
    38521 atgcgtaatt taccgctgat actggtggca tcctgttgaa acagaccgtc cagttcgttg
    38581 agattactca acacatcttt tgcccgttga taataggtca tgccttctgg cgtgagtttg
    38641 acccgtcgcg tggtgcgctg gagaagctgg gtgccaagtt gatgttccag tgcctgtatc
    38701 tggcgcgaaa cacttccctt tggcaaagca aagaaatccg ctgcgcggga aaaactttcc
    38761 agctccgcga ctttgatgaa caactgcatt gcgtgaattt tatccatttc tttgaccgat
    38821 tgttgttcac agcgaaacag taacgcgcat atcgcattgt ttattgtttt ttctaacatc
    38881 taatgccggg tttcacaatg ttgtcatgac gaatgtggcg atttctcagc aggaaaaggg
    38941 cggaatagct ttatgagaaa ggtgaatccc cttaaacaga gcgtattttt cagtgattcc
    39001 ctttaaagtg aatagaggac gtattggcta tttgtgctta tttttgtctc attgccattt
    39061 gttattttgg cttgtagtcg cggaatttca tttttaattg catgcgtact aaccaccaat
    39121 ataaggaaat cctcaaaatt gttttaaata gtgccctcga catgtaattt caggcgttta
    39181 gctgtaacta tttatatcgg taactatttt ctacgcgtca tctctgtaga atggccaccg
    39241 ccaaatggcg aattcttatt ttttctcgcg ggtaataccc ttccactact taagtctttt
    39301 taacatattc aggtggagat tcagttatgc aaatagtcat gtttgacagg cagtcaatat
    39361 ttattcatgg aatgaaaatc agtttacagc agcgtattcc aggggtgagt attcaggggg
    39421 ccagtcaggc agacgagtta tggcaaaagc tggaaagtta ccctgaagcc ttagttatgc
    39481 tcgatggcga tcaggatggt gagttttgct actggttgct gcaaaaaacc gtggtgcagt
    39541 ttcctgaggt taaggtgtta atcacggcga cggattgcaa taaacggtgg ttacaggaag
    39601 ttatccattt taatgtgctg gccattgtgc ctcgtgattc aaccgtcgag acatttgcgt
    39661 tggcggtaaa tagtgcggcg atggggatga tgtttctgcc tggagactgg cggacgacgc
    39721 cggaaaagga cattaaagat ctcaaatcgt taagcgcccg ccagcgggag attttaacca
    39781 tgttagcggc gggcgagtca aataaggaga tcggcagagc gctgaatatc agtaccggaa
    39841 cggtaaaagc gcatctggaa tccctgtatc gccgtctgga agtgaaaaat cgcacccagg
    39901 cggcaatgat gttaaatatc tcctcctgaa gaggataatt ttacagccag caggaagtta
    39961 gcggctggct gtaaaaatat tatggttcgc cgtacaaacc aattaaacgg cgtaccacac
    40021 cgttagtcca gccaaaccca tcctgcaacg gatactcgcc accgccgcct tcgcgtggaa
    40081 caccatcggc aatatggtat ttttcgatca gtttgtgctg ttccagatag aactgattca
    40141 ccgtcttcag ccagcttcgc gcgatttcat cacccagaag gtcatcgccg tacattttaa
    40201 atccctgaat cgccatccat tgtaacggtg cccagccgtt gggtttatcc cactgttcac
    40261 cggtttcgta ctcgcttgcc agaatcccgc caggtgtcag taaccgactg cgcacggcat
    40321 ttgccagacg atcggcctgt tcatggttcg ccataccgac atagagtggc acaatggcgg
    40381 cagcggaaaa cagcgccagt tgttcgcgtc gccagtcgta atcgcggtag atgccgtttt
    40441 catcatccca gaggtaacgc tttaccgcat cgcgacgggc actggccttc tggcggaaca
    40501 gtgcttctgt ctctttctcg cctttcagcg ccgagatgtt ggcgatggcg ctctccagtt
    40561 taaacaggaa ggcattcaga tcgatgggga tgaactgggt ggtacgaata ctcgccagac
    40621 gaccagtatc acgcagccaa cgggaagagt aatcccaacc ggaggccgcc cccgctcgta
    40681 aatcgcggta cacctcgttg ggcgggcgac cagaatgttt cgcggtttca acgtcctcaa
    40741 gccaggattc gtcacgcggc gtgtcgcgat cgtcccagta acggttgagc agcgatccgt
    40801 ccggcatccg cacaacatgg cgataggcct gattagggat caacgattct gcaccgtcca
    40861 tccagaaggc atattccatt ttcaggtggt cgagatagcg gcgcgcaccg cgtacaccat
    40921 cttcttcaaa caactccacc atcagcgcaa aaaccggtgg ttgcgagcgg ctcaaataat
    40981 aggtgcggtt gccgttgggg atgtgaccgt agttttcgat catccaggcg aagttatcgg
    41041 ccatgcattt cagcaaatct tcccgaccac tttccgccag ccccagcatg gtgaaatagg
    41101 aatcccaata gtaggtttcg ctaaaacggc cgcccgggac aatatatgac tgcggtagcg
    41161 ccagcagaga agaccacgga atgtgatcct gtggttcgcg ggttagcacc ggccacagct
    41221 ggtcgatatg ctctttcagg gaattttgcg ggtccgatac atactcgctg gagtagacct
    41281 ccggcagcca gaagtgattt tcaacaaact tgcgcaagtc aaaatcacga tgacggcgca
    41341 ctttacggta gcggattaaa atatccagcg ggtccatttt gggtgcgcag tcgggaaagg
    41401 ttttactgtc ggggaaaatt ttcgccgact gaacatgctc gaacagttcg agatagcgat
    41461 cggcaggcgt cagcgcatca gaggcgggca gcccttcaat catctcgggt tccggttctg
    41521 cctcgatcat ctcatccagt tttaattcat acgggtccag ttcatagcag agatcgactt
    41581 cgatcatcag ttcgtctgga ttagggtttt gaattttctg attgagcata aggatccaac
    41641 ctccgaaatt cgtcgctaat tatcggcttt tctttaaggg tagtcattcg ctccgcctgg
    41701 tgggcgacaa agcgtgcggt ggatcacgtt tattaacttg tcaaaaaata taacgcacta
    41761 atatcaatgg aatttataaa aatacggatt tggtgttgtt tttcattttc aaaacattca
    41821 atcctgctct cgccgcaatc atgatatgcc attcctgatc tgggtaatag gcacaggcta
    41881 tcttattgat agtttatatt catgtaattg attgattcct atcaaaaagc gtatctccct
    41941 gttttttatg atggctttgt ctcgcagaga cgtttgcagc attctggaaa attttctggg
    42001 tcacaataac tcaccaccgt gagggaagaa taatgaaaat ggtctcacgt attaccgcga
    42061 tcggcctggc tggcgtcgcg atttgctatt tagggttatc tggttatgtg tggtaccacg
    42121 ataataaacg cagtaaacag gccgatgttc aggcatctgc tgtcagtgaa aataataagg
    42181 ttttaggctt tctccgcgaa aaaggatgcg actattgcca cacgccttcg gcagaattac
    42241 ccgcctatta ttatattcct ggcgcgaaac agttgatgga ttacgacatt aagcttggat
    42301 ataaatcttt taacctcgag gccgtgcgtg cggcactgct ggctgataaa cccgtttcgc
    42361 aaagcgatct gaataagatt gaatgggtga tgcagtatga aactatgcca ccaacgcgtt
    42421 ataccgcgct acactgggcg ggtaaggtga gtgatgaaga gcgggcggaa atactggcct
    42481 ggattgcaaa acagcgcgcg gaatattacg ccagcaatga tactgctccg gagcatcgca
    42541 atgaaccggt gcagcccatc ccgcaaaaac tgcctaccga tgcgcaaaaa gtggcgttgg
    42601 gctttgcgct gtatcacgat ccccgtttat cggctgatag caccatttca tgcgctcatt
    42661 gccatgcgtt gaatgcgggg ggcgtcgatg gcagaaaaac atcgattggt gttggtggcg
    42721 cagttgggcc gattaacgcg ccgacggtat ttaactcagt atttaacgtt gagcagttct
    42781 gggatggtcg tgcggcaaca ttgcaggatc aggcaggtgg accgccgttg aacccgattg
    42841 aaatggcgtc gaaatcctgg gacgaaatta ttgctaagct ggaaaaagat ccgcagctta
    42901 aagcgcagtt cctcgaagtt tatccgcaag gtttcagtgg cgaaaatatt actgatgcca
    42961 ttgctgaatt tgagaaaaca ttaattacgc cggattcccc atttgataaa tggttgcgtg
    43021 gagatgaaaa tgcgctgacg gcgcaacaga aaaaaggcta tcaattattt aaagataata
    43081 aatgtgcaac ttgtcatggt ggtattattc tcggcggacg ttcctttgaa ccgttggggc
    43141 tgaaaaaaga ctttaacttt ggggaaatta cggcggcgga tattggtcgt atgaatgtga
    43201 ctaaagaaga gcgtgataaa ttgcgccaga aagtacccgg tttacgtaac gttgctttaa
    43261 cggcaccgta cttccatcgc ggtgacgtgc cgacgctgga cggggcggtg aaactgatgc
    43321 tgcgctatca ggtaggcaaa gagctgccgc aggaggatgt ggatgatatc gtagctttcc
    43381 tgcacagtct gaacggggtg tacacgccgt atatgcagga taaacaataa ctaatttgat
    43441 cgcccgaaca ggaatgtttg ggcgattttt attacgataa taaagtctgt ttttaatatt
    43501 atcatgttaa atgtttatat tataaaaagt cgtttttctg cttaggattt tgttatttaa
    43561 attaagcctg taatgccttg cttccattgc ggataaatcc tactttttta ttgccttcaa
    43621 ataaatttaa ggagttcgaa atggaccaga agctgttaac ggatttccgc tcagaactac
    43681 tcgattcacg ttttggcgca aaggccattt ctactatcgc ggagtcaaaa cgatttccgc
    43741 tgcacgaaat gcgcgatgat gtcgcatttc agattatcaa tgatgaatta tatcttgatg
    43801 gcaacgctcg tcagaacctg gccactttct gccagacctg ggacgacgaa aacgtccata
    43861 aattgatgga tttgtcgatc aa
//
LOCUS       DAJVUJ010000009        43224 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.009, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000009 DAJVUJ010000000
VERSION     DAJVUJ010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43224
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.009"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..255
                     /locus_tag="OQQ48_000532"
     CDS             <1..255
                     /locus_tag="OQQ48_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HCS1951411.1"
                     /translation="IRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQR
                     YFLALEEGVNLLPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(305..1561)
                     /gene="nupG"
                     /locus_tag="OQQ48_000533"
     CDS             complement(305..1561)
                     /gene="nupG"
                     /locus_tag="OQQ48_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HCS1951412.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(1763..2842)
                     /gene="mltC"
                     /locus_tag="OQQ48_000534"
     CDS             complement(1763..2842)
                     /gene="mltC"
                     /locus_tag="OQQ48_000534"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HCS1951413.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLQSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(2907..3182)
                     /locus_tag="OQQ48_000535"
     CDS             complement(2907..3182)
                     /locus_tag="OQQ48_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HCS1951414.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(3210..4262)
                     /gene="mutY"
                     /locus_tag="OQQ48_000536"
     CDS             complement(3210..4262)
                     /gene="mutY"
                     /locus_tag="OQQ48_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCS1951415.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            4423..5142
                     /gene="trmB"
                     /locus_tag="OQQ48_000537"
     CDS             4423..5142
                     /gene="trmB"
                     /locus_tag="OQQ48_000537"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCS1951416.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLNNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            5142..5468
                     /locus_tag="OQQ48_000538"
     CDS             5142..5468
                     /locus_tag="OQQ48_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HCS1951417.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            5652..6371
                     /locus_tag="OQQ48_000539"
     CDS             5652..6371
                     /locus_tag="OQQ48_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HCS1951418.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            6547..7593
                     /gene="ansB"
                     /locus_tag="OQQ48_000540"
     CDS             6547..7593
                     /gene="ansB"
                     /locus_tag="OQQ48_000540"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HCS1951419.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            7710..8717
                     /locus_tag="OQQ48_000541"
     CDS             7710..8717
                     /locus_tag="OQQ48_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HCS1951420.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQSAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(8872..10008)
                     /gene="hemW"
                     /locus_tag="OQQ48_000542"
     CDS             complement(8872..10008)
                     /gene="hemW"
                     /locus_tag="OQQ48_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HCS1951421.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRAEFSAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            complement(10001..10594)
                     /locus_tag="OQQ48_000543"
     CDS             complement(10001..10594)
                     /locus_tag="OQQ48_000543"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HCS1951422.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            complement(10602..10892)
                     /locus_tag="OQQ48_000544"
     CDS             complement(10602..10892)
                     /locus_tag="OQQ48_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HCS1951423.1"
                     /translation="MNAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            complement(10889..11455)
                     /gene="yggT"
                     /locus_tag="OQQ48_000545"
     CDS             complement(10889..11455)
                     /gene="yggT"
                     /locus_tag="OQQ48_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HCS1951424.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNILLPGMWMAL"
     gene            complement(11473..12177)
                     /gene="yggS"
                     /locus_tag="OQQ48_000546"
     CDS             complement(11473..12177)
                     /gene="yggS"
                     /locus_tag="OQQ48_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HCS1951425.1"
                     /translation="MNDIAHNLAQVRDKILAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            12195..13175
                     /locus_tag="OQQ48_000547"
     CDS             12195..13175
                     /locus_tag="OQQ48_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HCS1951426.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDALDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRHGISLALRLLPSHCPQ
                     LEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YIYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQRVGEGRL"
     gene            complement(13351..13767)
                     /gene="ruvX"
                     /locus_tag="OQQ48_000548"
     CDS             complement(13351..13767)
                     /gene="ruvX"
                     /locus_tag="OQQ48_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCS1951427.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(13767..14330)
                     /locus_tag="OQQ48_000549"
     CDS             complement(13767..14330)
                     /locus_tag="OQQ48_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HCS1951428.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTASADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            complement(14439..15389)
                     /gene="gshB"
                     /locus_tag="OQQ48_000550"
     CDS             complement(14439..15389)
                     /gene="gshB"
                     /locus_tag="OQQ48_000550"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HCS1951429.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVLLMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            complement(15402..16133)
                     /gene="rsmE"
                     /locus_tag="OQQ48_000551"
     CDS             complement(15402..16133)
                     /gene="rsmE"
                     /locus_tag="OQQ48_000551"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCS1951430.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGKIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            complement(16213..16920)
                     /gene="endA"
                     /locus_tag="OQQ48_000552"
     CDS             complement(16213..16920)
                     /gene="endA"
                     /locus_tag="OQQ48_000552"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HCS1951431.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            complement(17015..17512)
                     /locus_tag="OQQ48_000553"
     CDS             complement(17015..17512)
                     /locus_tag="OQQ48_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HCS1951432.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MENVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            complement(17589..18983)
                     /gene="galP"
                     /locus_tag="OQQ48_000554"
     CDS             complement(17589..18983)
                     /gene="galP"
                     /locus_tag="OQQ48_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HCS1951433.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            complement(19419..20573)
                     /gene="metK"
                     /locus_tag="OQQ48_000555"
     CDS             complement(19419..20573)
                     /gene="metK"
                     /locus_tag="OQQ48_000555"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCS1951434.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATTFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            complement(20877..21092)
                     /locus_tag="OQQ48_000556"
     CDS             complement(20877..21092)
                     /locus_tag="OQQ48_000556"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1951435.1"
                     /translation="MGITSAGMQSRDAECGERIFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            21228..21359
                     /gene="yqgB"
                     /locus_tag="OQQ48_000557"
     CDS             21228..21359
                     /gene="yqgB"
                     /locus_tag="OQQ48_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HCS1951436.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            21368..23344
                     /gene="speA"
                     /locus_tag="OQQ48_000558"
     CDS             21368..23344
                     /gene="speA"
                     /locus_tag="OQQ48_000558"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HCS1951437.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            23481..24401
                     /gene="speB"
                     /locus_tag="OQQ48_000559"
     CDS             23481..24401
                     /gene="speB"
                     /locus_tag="OQQ48_000559"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HCS1951438.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(24607..25365)
                     /gene="loiP"
                     /locus_tag="OQQ48_000560"
                     /gene_synonym="yggG"
     CDS             complement(24607..25365)
                     /gene="loiP"
                     /locus_tag="OQQ48_000560"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HCS1951439.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGIK"
     gene            25635..27626
                     /gene="tkt"
                     /locus_tag="OQQ48_000561"
     CDS             25635..27626
                     /gene="tkt"
                     /locus_tag="OQQ48_000561"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCS1951440.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIIGMTTFGESAPAELLFEEFGFTVNNVVAKAKELL"
     gene            27940..28383
                     /gene="cmtB"
                     /locus_tag="OQQ48_000562"
     CDS             27940..28383
                     /gene="cmtB"
                     /locus_tag="OQQ48_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HCS1951441.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISHR"
     gene            28411..29799
                     /gene="cmtA"
                     /locus_tag="OQQ48_000563"
     CDS             28411..29799
                     /gene="cmtA"
                     /locus_tag="OQQ48_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HCS1951442.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSLGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETESEDEFAQSTNAVKAMKQEGAFSLSRVKRIAFVCDTGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            29814..31091
                     /locus_tag="OQQ48_000564"
     CDS             29814..31091
                     /locus_tag="OQQ48_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCS1951443.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLVEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVDLAASKGIELVYVNTKGMSDPVQTLRALTGDVGFDDIFVYAAVPAVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPDTVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGIDIGEAAQ"
     gene            31088..32053
                     /gene="yggF"
                     /locus_tag="OQQ48_000565"
     CDS             31088..32053
                     /gene="yggF"
                     /locus_tag="OQQ48_000565"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCS1951444.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGVIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQESKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            32075..32584
                     /gene="fumE"
                     /locus_tag="OQQ48_000566"
     CDS             32075..32584
                     /gene="fumE"
                     /locus_tag="OQQ48_000566"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HCS1951445.1"
                     /translation="MAKLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQFWHYLNEQD
                     ETPGFADDMTWDFISNVNSITRNAMLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTILKELTP"
     gene            32581..33294
                     /locus_tag="OQQ48_000567"
     CDS             32581..33294
                     /locus_tag="OQQ48_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HCS1951446.1"
                     /translation="MKIELTVNGLKIQAQYQNEEIENVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(33266..33943)
                     /locus_tag="OQQ48_000568"
     CDS             complement(33266..33943)
                     /locus_tag="OQQ48_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HCS1951447.1"
                     /translation="MLTLNQISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(33937..34614)
                     /locus_tag="OQQ48_000569"
     CDS             complement(33937..34614)
                     /locus_tag="OQQ48_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCS1951448.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(34602..35309)
                     /locus_tag="OQQ48_000570"
     CDS             complement(34602..35309)
                     /locus_tag="OQQ48_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCS1951449.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLRILVIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            complement(35310..35888)
                     /locus_tag="OQQ48_000571"
     CDS             complement(35310..35888)
                     /locus_tag="OQQ48_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCS1951450.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(35911..36342)
                     /locus_tag="OQQ48_000572"
     CDS             complement(35911..36342)
                     /locus_tag="OQQ48_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCS1951451.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYARQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            36714..37733
                     /gene="epd"
                     /locus_tag="OQQ48_000573"
     CDS             36714..37733
                     /gene="epd"
                     /locus_tag="OQQ48_000573"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HCS1951452.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            37783..38946
                     /gene="pgk"
                     /locus_tag="OQQ48_000574"
     CDS             37783..38946
                     /gene="pgk"
                     /locus_tag="OQQ48_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCS1951453.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            39161..40240
                     /gene="fbaA"
                     /locus_tag="OQQ48_000575"
     CDS             39161..40240
                     /gene="fbaA"
                     /locus_tag="OQQ48_000575"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HCS1951454.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLSFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            40598..41458
                     /gene="mscS"
                     /locus_tag="OQQ48_000576"
     CDS             40598..41458
                     /gene="mscS"
                     /locus_tag="OQQ48_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HCS1951455.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            41597..42232
                     /gene="argO"
                     /locus_tag="OQQ48_000577"
     CDS             41597..42232
                     /gene="argO"
                     /locus_tag="OQQ48_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HCS1951456.1"
                     /translation="MFSYYFQGLDLGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            42325..43065
                     /locus_tag="OQQ48_000578"
     CDS             42325..43065
                     /locus_tag="OQQ48_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HCS1951457.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDVVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
BASE COUNT        10569 a        10783 c        11520 g        10352 t
ORIGIN      
        1 attcgcggtg aagtggagtt ggtgcggatt cgtgatgccg aagggcgaat tgcggcagaa
       61 ggggcgttgc cttatccacc tggcgtgctt tgcgtggtac ccggggaagt ctggggtggg
      121 gcggttcaac gttatttcct tgcactggaa gaaggggtaa atttgttgcc gggattttcg
      181 ccggagctgc aaggtgttta tagcgaaacc gatgcggatg gcatgaaacg gttgtacggt
      241 tatgtgttga agtaagaata aaaaaaacgg gtcaccttct ggcgacccgt ttttctttgc
      301 gtaattagtg gctaaccgtc tgtgtgcctg tcgggacacg aacgtgttta tatttgaaca
      361 tcgccatgaa cgcgaaggcc agaaccacgg agtaaccagc gaaaatcaac catacggtct
      421 gccagtcggt aatgccgttt tgggtgtaca tctcaacaac tttaccgctc acgatgccgc
      481 cgaggataca gccgaagccg ttagtcatca tcaggaacat cccttgtgca ctggcgcgaa
      541 ttgccgggct aacttctttt tcgacaaaca ccgaaccaga gatgttgaag aagtcgaatg
      601 cgcaaccgta aacgatcatc gacagtacca gcagtacagt accgaacgga gtcgggtcgc
      661 cgtaagcaaa cagcgcaaaa cgcaggatcc acgccacaat actgatcatc attacgttct
      721 taataccgta gcggcttaag aagaacggga tggtcagaat gaacagggtt tcagagatct
      781 gcgaaatcga catgatgatt gacgcatgct gcacaataaa gctgctggca aacatcggat
      841 ctttgtcgaa gctgtgcagg aaggtattac cgaacatgtt ggtaatctgc agttccgcgc
      901 ccagcagcat tgagaagata aagaagattg ccatacgctt gtttttaaac agcgcgaatg
      961 catcgaggcc cagcagggtt gtccagctct gattcgcttg ctgtttagca accggaatat
     1021 gcggcagagt cagggtaaac agaaccagaa tggcggaaag tgctgcgcca atatacagct
     1081 gcatgtggct taattcgaag ccagacaggc tcaccaccca cattgccatg ataaagccga
     1141 tggtgcccca gatacggatt ggcgggaagt cagtaacgat atccatcccg gcattttgca
     1201 ggcgatagta agagatggtg ttgattaacc caagcgttgg catataagca aacgagttaa
     1261 tcaatatcac aaggaacatc gcctccggtg tcgtgacctg tgccgccatg aacagcgtga
     1321 tagcgccaat ggtgtggcaa atggcatata cccatttcgc acttaaccat ttgtcggcca
     1381 caatccccag cagcgcaggc ataaagaccg ctgcgatacc cagtgagcta taaactgcgc
     1441 caatagaagc accgtcaaac ttcagggtaa caaacatata ggagccgagg gtcgtcagcc
     1501 aacttcccca cagacagaac tgcagaaaag agaggatttt cagctgcagc ttaagattca
     1561 tgttaatttc ctcacatcgt gatgcggatg agtgtttgaa aaagcacatt tgaccgtgcg
     1621 agatgatggg attctatcaa cttcgcggac tggttttttg ttacctgtgg caaataattg
     1681 caaacattcc caatttgcaa aacgaaattg tgatgtggat aacatttttg cccctgagca
     1741 tcgtcagggg cggttaatgg aattatcggc ggcggtagga tttttgcgcg gtattcactt
     1801 tataaagata acggcgagat tccgcagagg gatggcgggt cgtcagcgtc tgataaacat
     1861 cgcccggcgt catggtgtta ataatattgg cagcctgaat cttatcattc gaaaagactc
     1921 gcagcacgct gcctgcgccg ccgttatagg cggtgatgac ggcataacgc cgcgatgttg
     1981 ggttatcaat tccgccgaga taaacattgt tcagcatcgc cagatacgcg gtgccggtat
     2041 caatattgct ggcaggatca aacaagaaac tgcggctcgg cgtgccggat ttcccctgcg
     2101 agcggaacac atctttcccg gcagtatgtt gtaccacctg cattaatccc agcgcatcgg
     2161 aacggctgac cgcatacggg ttaaaggaag attcggtctg cataattgcc agaatcagcg
     2221 actcatcaac gccatatttc cgtgacgcct ggcggaccat gccgagatat ttgtgcgcac
     2281 gtttatcaag gtggttcggt accatgttaa tggtgacgct gtagatgata cgcagcccgt
     2341 tactgcggct ttgcagacgg tttttcagca gataatccgc gaagttgctt gcgcgacctt
     2401 cccagcgaat cggctgcccg gtgttgtcca ccacctgacc gtaaaggaaa ggttctttcg
     2461 aaatcgtaat atcatcaacg tcggaataga gatcgaccga actcggatcg tcacccatca
     2521 gcaacgtttt gataattgcc cggcgcaaat gcgcggcagg ttctgtcccg gcgatagttt
     2581 cgatagtaat cgtaccgtca tcgaagttga tgtggctgcg ggtctgatat tgatcggtgt
     2641 atttcacgta gtccttagga ccggcgatca ccacctcttt gaatccccag atgttctcaa
     2701 tattgtgggc aaattgcccc atcagaatat caaaaccgtt ggtatctttg acccaggctt
     2761 cgttataggt atcgcctttt ttggtcgtcg aacaggagat gagcaacggc gcaatcaaag
     2821 ccagcgcgag atattttttc atcattccgg gtgcgtgttg tgtttgtttt tatgcaagtt
     2881 gccggaggcg tgctccggca ctgtttttat tttttatctt ccggcgtata gccctcgata
     2941 tgcacctctt taccctcgaa caggaagttg accatctcct gctcaagcag cttgcggtgc
     3001 tcggcattca tcatgttgag tttcttttca ttaatcagca tggtttgctt gtgctgccac
     3061 tgcgcccagg cttctttgga gatctcgtta tagatgcgtt ttcccagctc gccggggtac
     3121 agctgaaaat cctgaccttc tgcttcacgt tgcaggaaag tacaaaaaat cgttctgctc
     3181 ataaatcatc ctctttatcg actcacgcgc taaaccggcg cgccagtgcg taactgctgt
     3241 aacaaacgct ccacgggagc cgccaggcca actgacggcg gttgcgctaa gttataccag
     3301 agcgcattgc cttcatccat gcagccggtg aatgacgaca cgggaagcca cataggcaca
     3361 atatctaagt ggaaatggct gaaggtatgc cgaaacgcgg tcagttgcgt caggttatcg
     3421 gcagcaatct gccgttgcgc cagccactgc cgcaaacttt cttcgtcggc aaactgcggg
     3481 aaacagtata aaccgcccca caatccgctc ggcggacgct gcgccagcaa tacttcatct
     3541 tcgtgctgta acagcaaaaa gtagccggtg cgctccggca gcgtctgttt cggttttttg
     3601 cccggataaa gcgaccagct attattggcg gcggcaatac atccgttttg tagcggacag
     3661 agcgaacatt tcggcttcga gcgcgtacaa atcatcgcgc ccaaatccat catcgcctga
     3721 ttaaaccgtt ccacgccgac cgcgggcgtc acctgctcgc tcaaactcca taatttattc
     3781 tcgacctctt ttttcccagg ccagccgctt acagcatagc agcgcgccag cacgcgtttg
     3841 acgttaccgt cgagaatcgg aaagtgctta cccagagaaa gcgagagaat cgcgcctgcg
     3901 gtggaacgcc cgacgcccgg taacgccgcg acttcttcaa aggtttccgg gaatttaccg
     3961 ccgtgtaagg tcgccacttg ttgtgccgct ttatgcagat tgcgcgcgcg ggcgtaatag
     4021 ccaagcccgg tccacaagtg gagaacttcg tcgagcggcg cattggcgag atcggtcacc
     4081 gtcgggaagc gcgccataaa gcgttcaaaa taggggataa cggtcgcaac ctgagtttgt
     4141 tgcaacatca cttctgagag ccatactttg tagggcgtct tgtcaatttg ccagggcagc
     4201 gtttttcgcc cgtatttatc gtaccagtcc agaacctggg ctgaaaattg cgacgcttgc
     4261 atggtcaccg aattcactgt tgttgggggc aagattgcag cacagcgacg gcagggtgta
     4321 aaccggaact ttccgcagcc acggccttat catgcttgca tccggcaact aactttggat
     4381 aatgcccgtt ttcagaacac tttcacaagc gactaaatct ttatgaaaaa cgacgtcatt
     4441 tcaccggaat ttgatgaaaa cggccgccca ctgcgccgta tccgtagttt tgtgcgccgc
     4501 caggggcgac tgaccaaagg ccaggaacat gcgctggaaa actactggcc ggtgatgggc
     4561 gttgagttca gcgaagatat gctggatttc cccgcgcttt ttggccgtga agcgccggtg
     4621 acgcttgaga ttggttttgg catgggggcg tcgctggtgg caatggctaa agatcgccct
     4681 gagcaggact tcctcggcat tgaagtgcat tcaccgggcg ttggtgcgtg cctggcttct
     4741 gcgcatgaag aaggtttaaa caacctgcga gtgatgtgtc acgatgcggt tgaagtgctg
     4801 cataaaatga ttcctgacaa ttcattgcgc atggtgcagc tctttttccc tgacccgtgg
     4861 cacaaagcgc gccataataa acgccgtatc gtacaggtgc cgtttgccga actggtaaaa
     4921 agcaaactgc agctgggggg cgtattccat atggcgaccg actgggaacc ttatgcggaa
     4981 catatgcttg aagtgatgtc ttctattgac ggttataaaa acctgtcaga gagcaatgat
     5041 tacgtaccgc gtccggcatc acgtccggtg acgaaatttg aacaacgtgg tcatcgtctt
     5101 ggtcacggag tatgggactt aatgttcgag agggtgaaat aatggcaaag aaccgtagcc
     5161 gtcgtctgcg taaaaaaatg cacatcgacg aattccagga attaggattt tcggtggcat
     5221 ggcgattccc ggaaggtaca tcggaagaac agattgataa aaccgttgat gattttatta
     5281 acgaggttat cgaaccgaac aaactggcct ttgacggcag cggttatctg gcctgggaag
     5341 gtctgatctg catgcaggaa atcggcaaat gcaccgaaga acatcaggcg attgtgcgta
     5401 agtggctgga agagcgcaaa ctggatgagg tacgcaccag cgaacttttc gacgtttggt
     5461 gggactaaga aagcatacgg gcgatgacaa atgcaaaact gcctgatgcg ctacgcttat
     5521 caggcctgga aagatgcacg atcgagtagg cgggataagg tgtttacgcc gcatccggca
     5581 tggaaaacgc gtactttgtt atcaatctgg ggccagcaaa tgctggcctg attttttctt
     5641 gagggaagac tatgatgcgc aaaatgctgc tggcggcagc actttcagtg acggcaatga
     5701 ccgctcacgc cgactaccag tgcagcgtca cgccgcgtga cgatgtgatt gtcagcccgc
     5761 aaaccgtgca ggtgaagggc gaaaacggca atctggtgat cacgccagac ggcaacgtga
     5821 tgtataacgg taagcaatat tccctgaatg ccgcccagcg cgagcaggcg aaggattatc
     5881 aggctgaact acgcagcacg ctgccgtgga ttgatgaagg cgcgaaaagc cgcgtcgaaa
     5941 aagcccgtat tgcgctggat aaaattatcg ttcaggagat gggcgaaagc agcaaaatgc
     6001 gcagccgtct gaccaaactt gatgcgcaac tgaaagagca gatgaaccgc attatcgaaa
     6061 cgcgcagcga tggcctgacg tttcactata aagccattga tcaggttcgc gccgaaggcc
     6121 agcaattagt gaatcaggca atgggtggaa ttttacagga cagcattaat gaaatgggcg
     6181 cgaaagcggt gctgaaaagc ggcggtaacc cattacagaa tgtgctggga agcctggggg
     6241 ggctgcaatc ctcaatccaa accgagtgga aaaagcagga aaaagatttc cagcagtttg
     6301 gcaaagatgt ttgtagccgc gttgtgactc tggaagatag ccgcaaagcc ctggtcggga
     6361 atttaaaata atcctctatt ttaagacggc ataatacttt tttatgccgt ttaattcttc
     6421 gttttgttac ctgcctctaa ctttgtagag ctccaaaata tattcacgtt gtaaattgtt
     6481 taacgtcaaa tttcccatac agagctaagg gataatgcgt agcgttcacg taactggagg
     6541 aatgaaatgg agtttttcaa aaagacggca cttgccgcac tggttatggg ttttagtggt
     6601 gcagcattgg cattacccaa tatcaccatt ttagcaaccg gcgggaccat tgccggtggt
     6661 ggtgactccg caaccaaatc taactacaca gcgggtaaag ttggcgtaga aaatctggtt
     6721 aatgcggtgc cgcaactaaa agacattgcg aacgttaaag gcgagcaggt agtgaatatc
     6781 ggctcccagg acatgaacga taatgtctgg ctgacactgg cgaaaaaaat taacaccgac
     6841 tgcgataaaa ccgacggctt cgtcattacc cacggtaccg acacgatgga agaaaccgct
     6901 tacttcctcg acctgacggt gaaatgcgac aaaccggtgg tgatggtcgg cgcaatgcgc
     6961 ccgtccacgt ccatgagcgc agacggtcca ttcaacctgt ataacgcggt agtgaccgca
     7021 gctgataaag catccgctaa tcgtggcgtg ctggtggtga tgaacgacac cgtactggac
     7081 ggtcgcgatg taaccaaaac caacaccacc gacgtagcga ccttcaagtc tgttaactac
     7141 ggtcctctgg gatacattca caacggtaag attgactacc aacgtacccc ggcacgtaag
     7201 cacaccagcg atacgccatt cgatgtctct aagctgaatg agctgccgaa agtcggcatc
     7261 gtttataact acgctaacgc atccgatctt ccggctaaag cactggtaga tgcgggctat
     7321 gatggcatcg ttagcgctgg tgtgggtaat ggtaacctgt ataaatccgt gttcgacacc
     7381 ctggcaaccg ccgcgaaaaa cggcactgca gtagtgcgtt cttcccgcgt accgacgggt
     7441 gctaccactc aggatgctga agtggatgat gcgaaatacg gcttcgtcgc ctctggcacg
     7501 ctgaacccgc aaaaagcgcg cgtcctgctg cagctggctc tgacgcaaac caaagatccg
     7561 cagcagatcc agcagatctt caatcagtac taatcacctc gctccggtat cgtgccgggg
     7621 ctttttcact tcagacttac gtccattgcc aattttcatt accctaatga taatcaccgg
     7681 aataaattat ttcgcgtgag gtttttcggg tgaaaaagca atggattgtt ggcacggcac
     7741 tgcttatgtt gatgactggt aatgcctggg cagatggcga accgccaact gaaaatatct
     7801 taaaagatca attcaaaaag cagtatcacg gcattctcaa gcttgatgct atcaccttaa
     7861 aaaatcttga tgctaagggt aatcaggcca cctggtcagc ggaaggcgat gtctcttcca
     7921 gtgacgatct ctatacctgg gtcggtcagt tggcagatta cgaactgctc gaacagacct
     7981 ggacgaaaga taaaccggta aaattctcgg cgatgttaac cagtaaagga acgcccgcgt
     8041 ctggctggtc ggtgaacttt tactcttttc agtcggcagc cagcgatcgt gggcgggtgg
     8101 tcgacgatat caaaacgaat aataaatatc tgatcgtgaa tagcgaagat ttcaattatc
     8161 gctttagtca gcttgaatct gcgttgaata cccagaagaa ctcgattcct gcgctggaaa
     8221 aggaagtgaa agcgcttgat aagcaaatgg tggcggcgca gaaagcagcg gatgcatatt
     8281 ggggaaaaga tgcaaacggt aagcaaatga cccgcgaaga agcctttaaa aaaattcacc
     8341 aacagcgtga tgagtttaat aaacagaacg atagcgaggc gttcgcggtt aaatatgaca
     8401 aagaggttta tcaaccggcg attgcggcat gtcataaaca gagtgaagag tgttatgaag
     8461 tgccgattca gcagaaacga gatttcgata tcaacgaaca acggcgacag acttttctgc
     8521 aatcacaaaa actcagccgt aaattgcagg atgactgggt aacgctcgaa aaagggcaat
     8581 atccgttaac gatgaaagtt tcagaaataa acagtaaaaa agtcgcgatt ttgatgaaaa
     8641 ttgacgatat caaccaagct aatgaacgct ggaaaaaaga taccgaacaa ctgcggcgca
     8701 atggggtgat taagtaagtt tgatatagtg cacgatttgt agcaggatgc ggcgttgacc
     8761 ccgcatccgg caatcaacgt ctgatgcgac gcttgcgcgt cttatcagac ctacaaccgt
     8821 tgccacatgt aggctggata aggcgttcac gccgcatccg gcaatacaag tttactcagc
     8881 cagaaaaagc tccagcagcg aatttaaaaa cagcttccca tgttccgtta tctgccagta
     8941 atccgcacat tctgtgagat aaccctgggc aatcgcctcg tctaactgtg ggcgaatcac
     9001 atcttcgcaa agcccggtat acgcactaaa ctctgcgcgt ggcgcggcct ccagcaagcg
     9061 aaagcggttc ataaagaact caaacggctt atctgcggct tcgacatcac gctggctttc
     9121 cagatacctt ccttgcataa aaccacgagg atgacgcgtt ttagtggtac gcagaatgcg
     9181 cccatccggg aaggtgactt tgccgtgcgc gccgcagcca ataccgatgt agtcaccaaa
     9241 tcgccagtag ttgagattgt gctggcactg ataaccgggt ttggcgtaag cggaagtttc
     9301 atattgctga taacccgctg cggttaataa ctgatgcccc tgttcgaaaa tatcccacag
     9361 cgcgtcatcg tccggcagca ccggtggtcg cgaaccaaac agcgtattgg gttcgatggt
     9421 cagttgatac caggaaagat gcggcggatt cagttcaatg gcctggcgta gatcgccaag
     9481 cgcctcttcc agtgattgat ccggtagtcc atgcatcaaa tcaaggttaa agctacgtaa
     9541 ccctaaaccg ctcgccagct ttgccgctcg tttcgcttct tgcgggccat gaatgcgccc
     9601 aagtcgtttc agcttttctt cgctaaaact ctgcacacca atagagatgc ggttcacacc
     9661 agcacgctga taatcgacaa agcgatcggc ttctaccgta ccagggttcg cttccatagt
     9721 aatttctgca tccgctgcca gcggcaaacg cgcacgcacg ccgtccagca gcgtttgcat
     9781 cgccgggccg gaaagcaggc tcggcgtacc accgccaata aagattgtct ttacttcacg
     9841 gccctgagcg taagccacat cattgtccag atcgcaaagc agatgctgaa cataatcgtc
     9901 gtgcggcact tctcctttca acgcgtgaga gttgaaatcg cagtacgggc atttctgcac
     9961 gcaccacggg atgtgaatgt agagactcag cggcggtaat ttaaccatta cgtaaagcgt
    10021 ccagcagcag tttcaacgcc tgaccacggt gggaaatggc gctcttttct tcgcgggtca
    10081 gttcggcggc ggttttccct tcggaaggta cgaagaagat tggatcataa ccaaagccac
    10141 cagtgcccgc tggttcgcga gtaatcacgc ccggccagct gccgtggcat accagcggag
    10201 tcggatcttc cgcgtgacgc agatatacca gcacgcagtg gaaacgtgcc tgacgttggt
    10261 cgtccggtac gtctttcagt gtttccaaca gtttttgcag attcttttga tcggtcgcgt
    10321 cttcaccgga ataacgcgcg gagtaaatac ccggcgcgcc acccagcgca tctaccgcca
    10381 gaccagagtc gtcggcaatt gccggtaaac cggtcacttt tgccgcatgg cgcgctttca
    10441 gaatcgcgtt ttcgataaag gttaggccgg tttcttcagc ggaatcaacg ccgaggtctg
    10501 tttgtgccac gatatcaaga ccaaagtcgc taagcagcga cgccagctca cgcactttac
    10561 cggcattgcc ggttgcgagg acaacttttt gcataggata cctaattaat taacgccgcg
    10621 atttctggcg ggatttgttg cggattaatg attttaattt gtttgtggcg gccaagttcg
    10681 cctttttcaa tcaccacctg gcttttggca acccggaatt gcttaccgag aaacttcacc
    10741 agatgactgt tggcctggcc gtcaactggc ggcgcggtaa tggcgacttt aacttcgtcg
    10801 ccatgtaaac cgacaataga atcacggctg gctttcggct gaatatagag ccgtaaaacc
    10861 agaccgtcat cattaactgt tacggcattc ataacgccat ccacatcccc ggcagcagga
    10921 tgtttccggt tgcctgtaat acttctgcga cacccatatt gatgacatac agcagcagaa
    10981 cgaggatcat cggcgagaaa tcaatcccac ccattgccgg tagcaagcgg cgaatcgggc
    11041 gcagcagcgg atcggccagc tgaatcagca cgtattcaat cgggctacga ccctggctta
    11101 cccagctcat aatcgccatc accagcagga cccagaaaat cagcaagccg atggttttca
    11161 gcagaatcag taaaccggca atccagatga ttggcaggaa ggtcaccact ttaaacagca
    11221 caatggcttt gataaaactg agaatatagg caaccagcag cgaggcgctg tcaatgggac
    11281 ccattgccgg aataacgcgg cgcagtggcc cgataattgg ctgcgttacc ttcactacaa
    11341 actgtgagaa ggggttgtaa aaatcacaat gagcccactg catccagatg cgtaataaca
    11401 gcaccatggt atacagctca atgaccgttg aaagcaggaa agtcaacgta ttcatggcgt
    11461 tcctttaatt ccttattttt tagagtaatc acgcgcacca aaaattgcag tgccgatacg
    11521 aaccatcgtg ctacctgccg caatggcggc ttccatatcg tccgacattc ccagagagag
    11581 cgtgtcgata tgcgggtagc gcgttttcag tccggcaaat gctacagcca tttggcgtgc
    11641 aacttcaaac tgccttacat attctgactc aggcgcaggg attgccatca gcccgcgcag
    11701 acgtaaacgc ggtagttcag cgaccgccgc tgccagctcg tccagttctg ccagttgaat
    11761 cccggactta ctgttttcat cactaatatt aatttgaatc agtacgttaa gagggggaag
    11821 ttctgccggg cgctgatcgt tgaggcgggt agcaatgcgc aaacggtcga tggtatgaca
    11881 ccagtcgaaa tgctctgcca ccaggcggct tttattagac tgcaacgggc caataaaatg
    11941 ccattctaat cctgttacgc ccagttcctg aaagtggcga attttatcta ccccttcctg
    12001 aacgtagttt tcaccaaatt gacgctgccc ggcatcaatg gcttctgcga tggcgctcgc
    12061 aggttttgtt ttactgactg caagcagcgt aatttcttct ggagaacggc cgcaacgcgt
    12121 tgcagccgct aagattttgt cccggacctg tgccaggtta tgcgcaatat cgttcatttt
    12181 ccgaggatct tagtatgaat atggaagaaa ttgtggccct tagtgtaaag cataacgtct
    12241 cggatctaca cctgtgcagc gcctggcccg cacgatggcg cattcgcggg ctaatggaag
    12301 ctgcgccgtt tgatgcgctg gacgtcgaag agctactgcg ggagtggctg gatgacgatc
    12361 agcgggcaat attgctggaa aatggccagc tggattttgc cgtgtcgctg gcggaaaacc
    12421 agcgattgcg tggcagcgca ttcgcgcaac ggcatggcat ttcgctggcg ttacggctgt
    12481 taccttcgca ctgcccgcag ctcgaacagc ttggcgcacc aacggtattg ccggaattac
    12541 tcaagagcga gaatggcctg attctggtga cgggggcgac ggggagcggc aaatctacca
    12601 ccctggcggc gatggttggc tatctcaatc aacatgccga tgcgcatatt ctgacgctgg
    12661 aagatcctgt tgaatatatc tatgccagcc agcgatgttt gatccagcag cgggaaattg
    12721 gtttgcactg tatgacgttc gcatcgggat tgcgggccgc attgcgggaa gatcctgatg
    12781 tgattttgct cggagagctg cgtgacagcg agacaatccg tctggcactg acggcggcag
    12841 aaaccgggca tttggtgcta gcaacattac atacgcgtgg tgccgcgcag gcagttgagc
    12901 gactggtgga ttcatttccg gcgcaggaaa aagatcccgt acgtaatcaa ctggcaggta
    12961 gtttacgggc cgtgttgtca caaaagctgg aagtggataa acaggaagga cgcgtggcgc
    13021 tgtttgaatt attgattaac actcccgcgg tggggaattt gattcgcgaa gggaaaaccc
    13081 accagttacc gcatgttatt caaaccgggc agcaggtggg gatgataacg tttcagcaga
    13141 gttatcagca gcgggtgggg gaagggcgtt tgtgacgttg ccatgcccga tgcgcaagtt
    13201 tatagggcct ataaaggcaa taaatgtctg atgcgacgct aacgcgtctt atcagaccta
    13261 cagccgctgc cagatgtgtg ctggataagg cgttcacgcc gcatccggca agatggcggt
    13321 aacgactatc cgccaggctt ttagatcgcc ttaatatccc tgctcgaaat agctttcgag
    13381 aataataacc gcagaggcag agtcgacttt gcctttgttg agcgcccgat agccgccctg
    13441 ttcaaacaga ccggaacggg cttccacggt gctaagacgt tcgtcatgga gctttacttc
    13501 aacaccgaaa cgaccatgaa tacggttggc aaatttacgc gctctggcag tcaatggttg
    13561 ctcggtgccg tccatattca gcggcaaacc gacgatgatt tcgtccggct gccactcttt
    13621 cagtaaacgt tcgatgaggt tccagtccgg cgtaccgtcc tgtgctttaa ttgcaggcaa
    13681 agggcgagca gtgccggtaa tgcgttggcc gaccgctacg ccaatgcttt tggtgccgaa
    13741 gtcgaaggcg agtaaggttc cactcatcag gcgtgccctg ccacaccagg catggtgaga
    13801 atatccacac caatcagttt tgccgcctcg cgccagcggt cggcaatcgg cgttttgaac
    13861 agaatattca gatccgccga ggccgttagc cacgcgttat cgagaatttc ttgttccagt
    13921 tgaccttttt cccaggaggc atagcccaga gccaccaata cgtcagacgg ttgtttatcg
    13981 gtgccgagcg tttccagcac atcgcgggaa gtggtcatca ccgtgttgtc tgaaatgcga
    14041 atgctggaag caaaattgga gggcggagta tgcaaaataa acccgcgatc ttcagccagt
    14101 gggccgccga gcataaccgg tttatccaga cggattgatt catcacgcgg ctccggcgtg
    14161 atcttcagct tttccagaat cccttcaatt ttgagatttt ccagcggctt gttgacgatg
    14221 atccccattg caccattggt attatgttcg caaatgtaga ccacggaacg acggaaaatc
    14281 ggatcctgga gagcaggcat ggcaataaga aagtgatgct gtaaattcat tgtcagaggt
    14341 tctgtttcct ggttcaaaaa agcaacagcg cccagtatgc gtggaaagaa gggcgctgtc
    14401 actcagagtc tcaacgagat ccttctcgct aaggtgggtt actgctgctg taaacgtgct
    14461 tcgatggcat ccattaacat tccggtgatc gacaccggaa actctgcttc aatctcacga
    14521 atacaggttg ggctggtgac gttaatttca gtcagacggt cgccgatgat atccagacca
    14581 acaaaaatca gccctttttc tttcagcgtc ggcccgatct gacgggcgat tttccagtca
    14641 ctttccgtca gcggccgagg ttcaccgcga ccaccggcag ccagattgcc acgggtttcg
    14701 cccccctgcg gaatacgcgc caggcagtac ggtaccggct cgccatccac caccagcacg
    14761 cgtttgtcgc catctttaat ggctggcagg taattttgcg ccatgcagta gcgagtgcca
    14821 tgctcagtca gggtttcggc aatcacgccg aggtttggat cgccttcttt cacgcggaaa
    14881 atcgacgcgc cgcccatacc gtccagcggc ttaagaatga tgtcgctgtg tttctcccag
    14941 aacgctttta gctgcgcttt attgcgcgta accagcgttt ctggcgttaa gtcagagaac
    15001 caggcggtaa acagtttctc gttacagtcg cgcaggctct gcggcttgtt aacgatcagc
    15061 gtccctttct cttcggcacg ttccagaata taggtcgcgt agataaactc ggtatcaaac
    15121 ggcgggtctt tacgcatcag gagcacatcg agatcggcca gcggcagatc ctgttcaccg
    15181 acgaacgaaa accactcttc gtagttctgc ttcacgttca gcgtgcgggt atgggcgcga
    15241 gcttcgccat tgatcagata cagatcgccc atctccatat agtgaagttc gtaaccacga
    15301 cgctgtgctt ccagcaacat agcgaaactg gaatctttct tgatgttgat gtttgcgatg
    15361 gggtccatca cgatgccgag cttgatcatt attcttctcc gttagcccaa atcgccaaat
    15421 cgtacttgta gcgcggtaat ggcggtgagc gcagttgtct ctgtacgcaa aacgcgaggt
    15481 cccaacagga tatcagtaaa ttgatagcgg gcagtcatgg caatttcatc tgccgataaa
    15541 ccgccttccg ggccaatcag caggcggacg cgttcaaccg gtaacggcaa cgtattgatg
    15601 ctgttactgg cgcgcgggtg aagattcagt ttcagtcctt catcctgctc tgcacaccag
    15661 gcttccagat ccatcgctgg acggatttcc ggcacccggt tacgaccaca ctgctcacag
    15721 gcagcaattg caatcttctg ccactgctgg agcttcttgt tcagacgttc actatccagt
    15781 ttaacgccgc agcgctcaga aaaaagtggc gtaatgaggc ttacaccgag ttcgatcgat
    15841 ttctggatcg taaattccat tttttcacca cgcgacatca cctgaccgag gtgaatatgc
    15901 agcggagatt cgcgatcgtc gatcttgcct tccagcacct tcacttccac gctttttttg
    15961 ctggcgctgg taatttcggc gtcaaagacc tggttgctac cgtcaaacaa ttgcaacgcc
    16021 tgccccggcc ccatgcgcag tacgcgcccg atatggttgg cggcatcttc gcaaagcgcg
    16081 atgtgagaat ggctggtcag tggttcagga tgataaatgc gggggatacg catagttaaa
    16141 aatccgcgtc gtctccccac gcggttgtac gcgtggggta ggggttaaca aaaagagtcc
    16201 cgctagtgta ggttagctct ttcgcgcctg gcaagcgcgt tgcacatacg ggttatgatt
    16261 gccttgcacc ttcgcgatgc gttcatcgcg ctcgcactcc cagtcggtaa ccggatacat
    16321 cttgttccat gcgttgaaca gctgcgtttg ctggcgagag agtgtcaggt tgtattggtc
    16381 gcgcatatag aagtaggtgc gcgcaatggc accgcgtgca cgcgctggtg gttcggcagc
    16441 tttttctttg aaatcgacct tcatggcgca ttgaccgtac tggccttcgc cgccattcca
    16501 ctggctgtac ataaagttgc cgcgatcgcc attcacctca ccgactgacg gctgcaggtt
    16561 atgcatatcg ctttccatct tgcgatagac cggatcttta gcgcagtttt tacgtccacc
    16621 gtcctgccag cactggcgct ggtgaccgaa ctgccaggcg ggaacgacgt gttcccactc
    16681 tacgcggctg gctcggtttt catttttgcg cacctgatag ccgcacgatt gcagatcaac
    16741 aacgcctttt ttgccctgcc agttaatttt acatccgcaa taaaacgtac cgggcgcgtc
    16801 agcgtggact tttaccgccg cggctttcgc ctgagaaaaa ctattgatac cttcggccaa
    16861 cgccgggccg gaaaatgctg cgctcagtac caccgcagca atagacaaat aacggtacat
    16921 cacttactcc gtgttaaaac gagccagcaa cgtagcgaaa gttgttttgg tatgcaatca
    16981 gccagatcag gaaagttcct gatagttcag atggttattt tgcgaccagt tgttcgccgc
    17041 agtgaacaca gcgatagacg gcttcgccac gaactacgcg attatggcgg cgtacggtaa
    17101 gctgatgctc ctggcacttg cagcggtagg ggaaggtgtt gcgacgcacg gattgcagtt
    17161 cgaactgatg cgtacgacgg gcgggaacac ccagcacgtt ttccatcatc cacttccact
    17221 ctttgccatg tggtgctacg cgcccgaaat gtttccatac cagcaaatgt gccagttcgt
    17281 gcggcaccac ttcttcaata aaggcttcac tgttttccaa cagcaaaacg ggattgaggc
    17341 ggatttcata gctttccagc caggctgttc cggcggaggt gccgcgctgg gtataagaga
    17401 gttttggctc cgggtagtta cgccccaatt tcaggttagc ctgggcgagt ttttcccgca
    17461 ggcgacgcat aacggcctgt tggatggcga tagggagacg ggatgttttc atagcggcag
    17521 aggatagagc gaagaaaaga tgactgcaag aggtggcttc ctccgcgatg ggaggaagct
    17581 tggggagatt aatcgtgagc gcctatttcg cgcagtttac gacctttcat cagattacgt
    17641 tcaatatgtt ccagcgaaac gtgtttggtt tccggtacca gccacaatgt cagcaggata
    17701 aacagtacgt tcagagccgc atacacccag aaggtgttgg cgttacccag tgtgttgagc
    17761 atggtcagga acgttgcgcc aacgatcatg ttggcaatcc agttggtggc ggtagagcag
    17821 gtgatgccaa aatcgcggcc tttcagcggc tgaatttcgg agcacagtac ccaaatcagc
    17881 ggaccggcac tcatggcaaa accgacaata aacatcagca gcatggcgat ggcgaaatac
    17941 tgcgctgacg gagagtgaat accgatatgc atcattgtac cgagtacgcc catgccagca
    18001 gccatcacca ggaagcccag cgttagcgtt ggtttacgtc cccagcggtc aacaaggccg
    18061 attgcgataa aggtggcaag tacgttggtc aggccgacaa tcactgtccc ccacatttgc
    18121 tcggtagtgt tggtataacc cgccagttcg aagattttcg gcgcgtaata catgatgacg
    18181 ttcatcccgg tgaattgctg cattacctgt aacagtacgc caaggaacac cgcgcggcgg
    18241 aagttgctat tctctttaaa cagcgcccag ccactctgtt taacctgcaa actttcacgg
    18301 atttcatcca gttcgcgttt cgcttccgcg ctggtgtcac gcaggcgtag cagcacgcgt
    18361 tcggcatcaa caaaacggcg tttggcggca aaccaacgtg ggctgtctgg caggaagaag
    18421 acaccaatca gcagcaaaat tgccgggata ataatcacac ccagcatcca gcgccatgca
    18481 ccggtgtagc tgaaggcggt atcagaaaga taagcaccga ggatcccgat agtgatcatc
    18541 aactgataca tcgagatcat actgccacga attttttccg gcgcaatttc agagaggtac
    18601 agcggtgcgg tataagaggc cacacccacc gccagcccca gtagaacgcg ggaaagaatc
    18661 agtacttcaa cgtttggcgc agccgcagag aacagcgaac cggcaacaaa caaaattgcg
    18721 ccgatcatca ggctcttttt gcgcccgagt ttaaaggaga gccagccgct gcccaccgca
    18781 ccgactgccg caccgaacat catggagctt acgacccatt cttgcgtgtg cgaagtaatc
    18841 tggaattcat ctgcaataaa cggcagtgcg ccagcaatta caccgatatc caggccaaag
    18901 agtaatcccg ccagagcggc aaggaagcag acgaaaaacg tcattgcctt gtttgaccgc
    18961 ccctgttttt tagcgtcagg catgatgccc tccaatatgg ttatttttta ttgtgaatta
    19021 agataggtga gtacgacgta aaaagatgtg aagcaaatca catcagtgta atcgcttaca
    19081 ctaacattta tcttagttat ggtttaagtg catgaaaata attgagttat atcatcatag
    19141 attaaacgct gttatctgca attaagactt tactgaaaag aaatgtaaca actgtgaaaa
    19201 ctgaataata atatgaaaat attacaaata tttggctggt gttattggtg taatcggttt
    19261 cagtagtgat gataatttgc gataaaacgt gttgaggaag aatgatgtta ttgtgcgggc
    19321 agctttttgc cggatgcggc gtgaatgcct atccaccgcg gtgaggtaaa agaaaaaggc
    19381 cagcgcgagg ctggcctttg aaagcaggtg aagaaagatt acttcagacc ggcagcatcg
    19441 cgcagcagct gcgctttgtc ggttttttcc cacgggaaat gttcacgacc aaagtgaccg
    19501 tatgctgcgg tttctttgta gatcgggtgc agcagatcca gcatctgaat cagaccgtat
    19561 gggcgcaggt cgaagaactc acgtaccagc agagtcagtt gttcagaagg cactttctca
    19621 gtaccgaaag tttctaccat gatggaggtc ggttcagcca cgccgattgc gtaggaaacc
    19681 tgaatttcac aacgatcggc caggccagca gcaacgatgt ttttcgcgac ataacgtgct
    19741 gcgtaggctg cggaacggtc cacttttgat ggatctttac cagagaatgc tccgccaccg
    19801 tgacgtgcca tgccgccgta ggtatcaacg ataattttac gaccagtcag accgcagtca
    19861 cccatcgggc caccgataac aaaacgaccg gtcgggttga tgaagaatgt ggtggcagaa
    19921 gtcagccatt cagcgggcag aattggcttg atgatctctt ccattaccgc ttcttgcagc
    19981 gatttctggt cgatctcttc agagtgctga gtggaaagca cgacagcatc aataccaacg
    20041 attttgccgt cgtcatactg gaaagtcacc tggcttttcg cgtccgggcg cagccacggc
    20101 agagtgccgt ttttacgcac ttcagcctga cgctgtacca gacggtgtgc ataggtgata
    20161 ggtgctggca tcagcacgtc ggtttcatta gttgcgtagc caaacatcag accctggtca
    20221 cccgcgccct gttccagcgg atcggcacgg tcaacgccct ggttgatgtc aggagactgt
    20281 ttgccgatag cgctcagaac cgcacaggag ttagcgtcaa agcccatgtc ggaatgcaca
    20341 tagccaattt cgcgaacggt gttacgagtg atctcttcga tgtctaccca ggcgctggtg
    20401 gtgatttcgc cgccaactaa aaccatgccg gtttttacgt aggtttcgca agcaacgcgt
    20461 gctttcggat cctgttcgag gatcgcgtct aaaacggcat cagagatttg gtcagcaatt
    20521 ttgtcaggat gcccttcaga gacggactcg gacgtaaaaa ggtgttttgc catatttaat
    20581 atcacctaaa gagaatttgg ttagctcaaa ctgttgtgtg gattttctgt ggtagcggat
    20641 cctaccacga ctctgcaggt taaaaacact ggcagtctga gtgttaatcg gtatggatgg
    20701 attaacatct agatggctat tttaggtcaa ttcttcaccc gatttccact tttttttgaa
    20761 tcgtgtctca ttctgttaaa aacgtggctg gaaatttttt ctgacaatgc cggcattctg
    20821 cgtatttata ttttgcaatt ttctgccatt gtggggtata aaacgcggcg cgcggcttaa
    20881 ataaaaagca cacgacgttt ctttcgtgtt gccacttcca gccgggttga aatcagagtt
    20941 ttggcttgtg ggttcgtctt aacaggcggc cgtggaggtg atacgaaata atgaaccgta
    21001 gtctgctgct taacctgtct caccgttctg gtgaagattc gttccccgca ctctgcatct
    21061 ctgctttgca tacctgccga tgttataccc atctcggcgc ttctcaggat tcaagagctg
    21121 gttacagtta ctgaggactg aacaagggcg ctcttgtaaa aacaagagtt ttctcgtggt
    21181 ttcgccgaac tgtcacactt acgttcggtt atgtgcttaa taatgttatg aaaaagaaac
    21241 cggttgcgca gttggagcgt cagcattcac tgctggaaaa tccatgtgct tatgggttgt
    21301 tatcgcagtt ccaggctgcg atagtcgtta actgttttac acttaataaa ataatttgag
    21361 gttcgctatg tctgacgaca tgtctatggg tttgccttcg tcagcgggcg aacacggtgt
    21421 actacgctcc atgcaggagg ttgcaatgag ctcccaggaa gccagcaaga tgctgcgtac
    21481 ttacaatatt gcctggtggg gcaataacta ctatgacgtt aacgagctgg gccacatcag
    21541 cgtgtgcccg gacccggacg tcccggaagc tcgcgtcgat ctcgcgcagt tagtgaaaac
    21601 tcgtgaagca cagggtcagc gtctgcctgc actgttctgt ttcccacaga tcctgcagca
    21661 ccgtttgcgt tccattaacg ccgcgttcaa acgtgcgagg gaatcctacg gctataacgg
    21721 cgattacttc cttgtttatc cgatcaaagt taaccagcac cgccgcgtga ttgagtccct
    21781 gattcattcg ggcgaaccgc tgggtctgga agccggttcc aaagccgagt tgatggcagt
    21841 gctggcacat gctggcatga cccgtagcgt catcgtctgc aacggttata aagaccgcga
    21901 atatatccgc ctggcattaa ttggcgagaa gatggggcac aaggtctatc tggtcattga
    21961 gaagatgtca gaaatcgcca ttgtgctgga tgaagcagaa cgtctgaatg tcgttcctcg
    22021 tctgggcgtg cgtgcacgtc tggcttcgca gggctcgggt aaatggcagt cttccggcgg
    22081 ggaaaaatcg aagttcggcc tggctgcgac tcaggtactg caactggttg aaacgctgcg
    22141 tgaagctggg cgtctcgaca gcctgcaact actgcacttc cacctcggtt cgcagatggc
    22201 gaatattcgc gatatcgcca cgggcgttcg tgaatccgcg cgtttctatg tggaactgca
    22261 caagctgggc gtcaatattc agtgcttcga cgtcggcggc ggtctgggcg tggactatga
    22321 aggcacccgt tcgcagtctg actgctccgt aaactacggc ctcaatgaat atgccaacaa
    22381 catcatctgg gcgattggtg atgcgtgtga agaaaacggt ctgccgcatc cgacggtaat
    22441 caccgaatcg ggtcgtgcgg tgactgcgca tcacaccgtg ctggtgtcta atatcatcgg
    22501 cgtggaacgt aacgaataca cggtgccgac cgcgcctgca gaagatgcgc cgcgcgcgct
    22561 gcaaagcatg tgggaaacct ggcaggagat gcacgaaccg ggaactcgcc gttctctgcg
    22621 tgaatggtta cacgacagtc agatggatct gcacgacatt catatcggct actcttccgg
    22681 cacctttagc ctgcaagaac gtgcatgggc agaacaactc tatctgagca tgtgccatga
    22741 agtgcagaag caactggatc cgcaaaaccg tgcgcatcgt ccgataatcg acgaactgca
    22801 ggaacgtatg gcggacaaaa tgtacgtcaa tttctcgctg ttccagtcaa tgccggacgc
    22861 atgggggatc gaccagttgt tcccggttct gccgctggaa gggctggatc aagtgccgga
    22921 acgtcgcgct gtgctgctgg atattacctg tgactctgac ggtgctatcg accactatat
    22981 tgatggtgat ggtattgcca cgacaatgcc aatgccggag tacgatccag agaatccgcc
    23041 gatgctcggt ttctttatgg tcggcgcata tcaggagatc ctcggcaaca tgcacaacct
    23101 gttcggtgat accgaagcgg ttgacgtgtt cgtcttccct gacggtagtg tagaagtgga
    23161 actgtctgac gaaggcgata ccgttgcgga tatgctgcaa tatgtgcagc tcgatccgaa
    23221 aacgctgtta actcagttcc gcgatcaagt gaagaaaacc gatcttgatg ctgaactgca
    23281 acaacagttc cttgaagagt tcgaggcagg tttgtacggt tatacttatc tggaagatga
    23341 gtaagtccgt attacttgaa tccgcttaat ttagcggtga taatccgcca caatttattg
    23401 tgacaaatcc aacccttcct cgtcgggcct aacgacgcgg aagggttttt ttatatcgac
    23461 tttgtaatag gagtccatcc atgagcacct taggtcatca atacgataac tcactggttt
    23521 ccaacgcctt tggtttttta cgcctgccga tgaacttcca gccgtatgac agcgatgctg
    23581 actgggtgat tactggtgtg ccgttcgata tggccacttc tggtcgtgcg gggggacgcc
    23641 acggtccggc agcgatccgt caggtttcga cgaatcttgc ctgggaacac aatcgcttcc
    23701 cgtggaattt cgacatgcgt gagcgcctga acgtcgtgga ctgcggcgat ctggtatatg
    23761 cctttggcga tgcccgtgag atgagcgaaa aactgcaggc acacgccgag aagctgctgg
    23821 ctgccggtaa gcgtatgctc tctttcggtg gtgatcactt tgttacgctg ccgctgctgc
    23881 gtgctcatgc gaagcatttc ggcaaaatgg cgctggtaca ctttgacgcc cacaccgata
    23941 cctacgcgaa cggttgtgaa tttgaccacg gcaccatgtt ctataccgcg ccgaaagaag
    24001 gtctgatcga cccgaatcat tccgtgcaga ttggtattcg taccgagttt gataaagaca
    24061 acggttttac cgtgctggac gcctgccagg tgaacgatcg cagcgtggat gacgttatcg
    24121 cccaagtgaa acagattgtg ggtgatatgc cggtttacct gacctttgat atcgactgcc
    24181 tagatcctgc tttcgcgccg ggaaccggta cgccagtgat tggcggcctg acctccgatc
    24241 gcgccattaa actggtacgc ggcctgaaag atctcaacat tgttgggatg gacgtagtgg
    24301 aagtggctcc ggcatacgat cagtcggaaa tcaccgctct ggctgcggcg acgctggcgc
    24361 tggagatgct gtatattcag gcggcgaaaa agggcgagta agcaccagat gcgatgcgca
    24421 cgggtaaaac gtgccattaa tgtcggatgc ggcgtgaacg ccttatccga cctacgttcg
    24481 gcacccgtag gccggataag atgcgccagc atcgcatccg gcaatgcgca caaggtaaca
    24541 aatgtgccat taatgtcaga tgcggcgtga acgccttatc tgacctacgt tcgacaccac
    24601 caggctttac ttaatcccat ccgcgctcat gcgatcgcga atatgctggg cgcgttcggc
    24661 ggatgccgga tggtcgtcaa acattgagct ttggcggcct tcttccagtt tcgccagttt
    24721 ttcaaagctg gtggcaagac ctgccgggct gatgccgcgt tggcgcagaa gatcgtaaga
    24781 ataatcatcg gcttctgctt cctggcgctg ggagaattgc gaattgacta atttctcgcc
    24841 cagatcacca agttgtgatt gagataaact gccgacaatc ccgcccgcag aggccgcagc
    24901 tactcgcacg gcatttgtac caagcgccac ctgcatccct ttcttcacat ggcctaacgc
    24961 cacgtgcccc atctcgtgac cgatcaccgc ttcgacttcg ttatccgtca tcatatccat
    25021 cagcccgcta tagacgcgga tacagccgtt agccattgca aaggcgttca catccttcgc
    25081 catatacact ttgtaattca ccggctgacc gttgatattg ttgcctagcg cattggcaat
    25141 agttgtcaga cgtttagcgt attcgctatt ggctggcgca atcgtcgcct tgctgtccat
    25201 ctcctgacat gcctgatcgc tcagggtttt cacctgcgca tcactcaaac tgtaagcctg
    25261 aaaagcttcc gctcctgatg agagcagtcc gttggagtcc atattctggc aaccagtcag
    25321 taccgttgcc acgctcattg ctaccaataa ggcgcgaatt ttcatgtttt ctcttccgca
    25381 caaggtcagg attattctaa aaatgcgccg tttgcaggtg aatcgacgct cagtctcagt
    25441 ataaggaaaa gcgcagcgga cgggcgagta gattgcgcaa catgcgagca tgatccagag
    25501 atttctgaag cggcaaaagg atgttccatg tacatgacgc gcggcttgcg gtaaattgtt
    25561 ggcaaatttt ccggcgtagc ccaaaacgcg ctgtcgttaa gggcgtgccc ttcatcatcc
    25621 gatctggagt caaaatgtcc tcacgtaaag agcttgccaa tgctattcgt gcgctgagca
    25681 tggacgcagt acagaaagcc aaatccggtc acccgggtgc ccctatgggt atggctgaca
    25741 ttgccgaagt cctgtggcgt gatttcctga aacacaaccc gcagaatccg tcctgggctg
    25801 accgtgaccg cttcgtgctg tccaacggcc acggctccat gctgatctac agcctgctgc
    25861 acctcaccgg ttacgatctg ccgatggaag aactgaaaaa cttccgtcag ctgcactcta
    25921 aaactccggg ccacccggaa gtgggttaca ccgctggtgt ggaaaccacc accggtccgc
    25981 tgggtcaggg tattgccaac gcagtcggta tggcgattgc agaaaaaacg ctggcggcgc
    26041 agtttaaccg tccgggccac gacattgtcg accactacac ctacgccttc atgggcgacg
    26101 gctgcatgat ggaaggcatc tcccacgaag tttgctctct ggcgggtacg ctgaagctgg
    26161 gtaaactgat tgcgttctac gatgacaacg gtatctctat tgatggtcac gttgaaggct
    26221 ggttcaccga cgacaccgca atgcgtttcg aagcttacgg ctggcacgtt attcgcgaca
    26281 tcgacggtca tgacgcggca tccatcaaac gcgcagtaga agaagcgcgc gcagtgactg
    26341 acaaaccgtc cctgctgatg tgcaaaacca tcatcggttt cggttccccg aacaaagccg
    26401 gtactcacga ctcccacggt gcgcctctgg gcgacgctga aattgccctg acccgcgaac
    26461 agctgggctg gaaatatgca ccgttcgaaa tcccgtctga aatctatgct cagtgggatg
    26521 cgaaagaagc aggccaggcg aaagaatccg catggaacga gaaattcgct gcttacgcga
    26581 aagcttatcc gcaggaagcc gctgaattta cccgccgtat gaaaggcgaa atgccgtctg
    26641 acttcgacgc gaaagcaaaa gagttcattg ctaaactgca ggctaacccg gcgaaaatcg
    26701 ccagccgtaa agcgtctcag aatgctatcg aagctttcgg tccgctgttg ccggaattcc
    26761 tcggcggttc tgctgacctg gcaccgtcta acctgaccct gtggtctggt tctaaagcaa
    26821 tcaacgaaga tgctgcgggt aactacatcc actacggtgt tcgcgagttc ggtatgaccg
    26881 cgattgctaa cggtatctcc ctgcacggtg gtttcctgcc gtacacctcc accttcctga
    26941 tgttcgtgga atacgcacgt aacgccgtac gtatggctgc gctgatgaaa cagcgtcagg
    27001 tgatggttta cacccacgac tccatcggtc tgggcgaaga tggcccgact caccagccgg
    27061 tagagcaggt cgcttctctg cgcgtaaccc cgaacatgtc tacatggcgt ccgtgtgacc
    27121 aggttgaatc cgcggtcgcg tggaaatacg gtgttgagcg tcaggacggc ccgaccgcac
    27181 tgatcctctc ccgtcagaac ctggcgcagc aggaacgaac tgaagagcaa ctggcaaaca
    27241 tcgcgcgcgg tggttatgtg ctgaaagact gcgccggtca gccggaactg attttcatcg
    27301 ctaccggttc agaagttgaa ctggctgttg ctgcctacga aaaactgact gccgaaggcg
    27361 tgaaagcgcg cgtggtgtcc atgccgtcta ccgacgcatt tgacaagcag gatgctgctt
    27421 accgtgaatc cgtactgccg aaagcggtta ctgcacgcgt tgcggtagaa gcgggtattg
    27481 ctgactactg gtacaagtat gttggcctga atggcgctat catcggtatg accaccttcg
    27541 gtgagtcagc tccggcagag ttgctgttcg aagagttcgg cttcaccgtc aacaacgtcg
    27601 ttgcgaaagc aaaagaactg ctgtaattag catttcgggt aaaaaggtcg cttcggcgac
    27661 cttttttatt accttgatat gtccgtttgc ggacaagcaa tagataaagc gtgttgcaga
    27721 tcacaaatat ttatatgcaa taaatatcaa ttatgtaata tgcatcacga tatgcgtatt
    27781 gacatttgtt gttatatcta taactcaatg ttatataaga aattaataat tcactgtttt
    27841 caaaacaccg gtttccctgc tcaattgctt tcattaaacc gcacgagtat tttattttta
    27901 ccgccggacg gtaccctaaa aataaaataa ggataattta tgcggcttag tgactatttt
    27961 ccagaatcat caatctcagt tatacattca gcaaaagatt ggcaggaagc tatcgatttc
    28021 tcgatggtat cattgctgga taaaaactat atcagcgaga attacattca ggcaattaaa
    28081 gattccacca ttaacaatgg cccttattat attctcgcac caggcgtggc aatgcctcat
    28141 gcgcgaccgg aatgtggggc gcttaaaacc gggatgtctt tgacattact tgaacaaggt
    28201 gtttattttc cggggaatga cgaaccaatt aaattactca tcggactctc tgctgccgat
    28261 gccgattcgc acattggcgc tattcaggcg ttaagtgaat tactgtgcga agaagaaata
    28321 ctcgaacaac tcttaacagc atcatcagaa aaacaattag cggacattat cagccacaga
    28381 taattactct ctcctctaca ggataatatt atggaaaaca agtctgctcg tgcaaaggtc
    28441 caggcttttg ggggcttttt gactgcaatg gtcatcccca atattggtgc ttttattgcc
    28501 tggggtttta ttactgcgtt atttattccc accggttggc tgcctaacga acatttcgcc
    28561 aaaattgtcg gcccgatgat tacctattta ttgcccgtga tgattggttc tacaggtggt
    28621 catctggtcg gcggtaaacg cggggcggtc atgggcggaa taggtactat tggtgtgatc
    28681 gttggcgcag agatcccgat gttccttggc tcaatgatca tggggccgct cggtgggttg
    28741 gtcataaaat atgtcgataa ggcactggaa aaacgcatac ctgccggttt tgagatggtt
    28801 atcaataact tctcattagg tatcgcgggg atgctccttt gtctgctggg ttttgaagtg
    28861 atcggcccgg cggtgttaat tgccaatact ttcgtcaaag agtgtatcga ggcgctggta
    28921 catgcgggtt atctgcctct gctgtcagtc atcaatgaac cggcgaaagt gcttttcctt
    28981 aataatgcga tcgatcaggg cgtctattat ccgctgggaa tgcaacaggc ttcggttaac
    29041 ggtaaatcca tcttctttat ggtggcctct aacccaggtc cgggcctggg gctgctgctg
    29101 gcgtttacct tgtttggtaa agggatgagt aaacgttctg cgcccggggc gatgattatt
    29161 cacttcctcg gtgggatcca cgaactgtat ttcccatatg tgctgatgaa gccgctgact
    29221 attattgcca tgattgcggg cggtatgtct ggtacctgga tgtttaactt actggacggt
    29281 ggtctggtgg ctggcccaag tctggggtct atctttgctt acctggcact gacgccgaaa
    29341 ggctcgttcc tggcgacaat tgccggtgtt acggtaggta ccctggtgtc ctttgctatt
    29401 acttcgctga tactgaagat ggaaaaaacg gtggaaacgg agagcgaaga tgagtttgct
    29461 cagtcaacca atgcggttaa ggcgatgaaa caagagggtg cattctcgtt aagcagggtt
    29521 aagcgtatcg cctttgtttg cgatacgggg atgggctcca gtgcgatggg cgcgaccacc
    29581 ttccgtaaac gcctggaaaa agcggggctg gcaatagaag taaaacatta cgcgatagaa
    29641 aacgtgcctg cggatgccga tatcgtcgtc actcacgcca gtctggaagg gcgcgtgaaa
    29701 cgtgtgacgg ataaaccgct gatattgatt aataactata ttggcgatcc aaaactcgac
    29761 actttattta atcaattaac cgccgaacat aaacactgat tacagaggta aaaatgaaaa
    29821 ccaaagttgc tgctatttat ggcaagcggg atgtccgtct gcgcgaattt gaactgccag
    29881 aaattaccga taatgaactg ttagtgagtg taatttctga cagcgtctgt ttatcgacct
    29941 ggaaagcggc gttactcggt agtgaacata aacgcgtacc cgacgattta gaaaatcatc
    30001 cggtcattac cgggcatgaa tgtgccgggg ttattgtcga agtgggtaaa aatctcactg
    30061 gcaaatataa aaaaggccag cgttttgtat tgcaaccggc gatggggtta ccaagcggat
    30121 attcagcagg ctacagctac gaatattttg gcggtaacgc cacctatatg attattcccg
    30181 aaatcgccat taatttgggc tgcgtattac cgtatcacgg ctcttatttt gctgcggcgt
    30241 cgctggtaga gcctatgtgc tgcattattg gtgcttatca tgccaattat cacaccacgc
    30301 aatatgttta tgagcatcgc atgggcgtca aacctggcgg caatattgca ctgctggcgt
    30361 gtgcgggtcc gatgggcatt ggcgctatcg attacgccat taacggtggc atacaaccgt
    30421 cacgggtggt ggtggtcgat atcgacgaca aacgcctggc gcaggtgcag aagctgctgc
    30481 cggtggatct ggcggccagt aaaggcattg agctggtgta tgtgaatacc aaagggatga
    30541 gcgatccggt ccagacgctg cgggcgctga caggagatgt cgggttcgat gacatttttg
    30601 tttatgcggc ggtgcctgct gtcgttgaga tggctgacga attactggcg gaagatggct
    30661 gtctgaactt ctttgccggg ccgacggata aaaacttcaa agtgccgttt aatttctaca
    30721 acgtccatta caacagcacg cacgtagtcg gtacatccgg cggttcaacg gacgacatga
    30781 aagaggcgat tgcgttaagc gccacggggc agttgcagcc gtcctttatg gtcacgcata
    30841 tcggtgggct ggatgcggtg ccagataccg tgctcaatct gccggatatc cctggcggta
    30901 aaaaactcat ttataacggc gtgaccatgc cgctcactgc cattgccgat tttgccgaaa
    30961 aaggcaaaac cgatccgcta tttaaagagt tggcgcggct ggttgaggaa acgcacggca
    31021 tctggaatga acaggccgag aaatatctgc tggcacaatt tggcattgat atcggggagg
    31081 ccgcgcaatg atgtccctgg cgtggccatt atttcgcgtt acggaacagg cagcgttggc
    31141 tgcctggccg caaaccggat gtggcgacaa aaataaaatt gatggcctgg cggtcaccgc
    31201 gatgcgccag gcattaaacg acgtcgcttt tcgtggacga gtggttatcg gcgaaggaga
    31261 aattgaccat gcgccgatgc tgtggattgg cgaagaggtg ggcaaaggcg atgggccaga
    31321 agtcgatatc gcggttgatc ccattgaagg cacgcggatg gtggcgatgg ggcagagcaa
    31381 tgcgctggcg gtcatggctt tcgcccctcg ggatagcctg ttacatgcgc ccgatatgta
    31441 tatgaaaaag ctggttgtta atcgactggc tgccggggtg attgatctgt ctttgcctct
    31501 ggcggacaac ttgcgtaacg tggcgcgggc gttaggtaaa ccgctggaca agctgcgcat
    31561 ggttacgctg gataaaccgc gcctgagtgc cgcgattgag gaagcaacac agctgggcgt
    31621 gaaggttttt gccctgccgg atggcgatgt cgccgccagc gtgctgacct gctggcagga
    31681 taatccttac gatgtgatgt acaccatcgg cggtgcgcca gaaggcgtga tttctgcctg
    31741 tgccgttaaa gcattaggcg gggatatgca ggccgagctg attgactttt gtcaggctaa
    31801 aggggattac acggaaaatc ggcagattgc agagcaggag agcaagcgtt gcaaggcaat
    31861 gggcgttgac gttaaccgtg tgtactcgct cgatgaactg gtgaggggga acgatatcct
    31921 ctttagcgct acgggcgtga cgggcggcga gttggtgaac ggtatccaac agacggcgaa
    31981 tggggtgcgg acgcagacat tactgatcgg cggcgcggac caaacgtgta atataataga
    32041 ctccctgcat tgatggtgat ctatcggaag accaatggcg aagctgacag aagatgatgt
    32101 gcttgagcaa ctggatgcac aggacaattt attttcattt atgaaaactg cgcattctat
    32161 tttgctccag gggatacgcc agtttctgcc gtcgctgttt gtcgataacg atgaagagat
    32221 cgtggaatat gcagtgaagc cgttactcgc ccaaagcggc ccgcttgacg atattgatgt
    32281 tgcgctgcgt ttgatttatg cgctggggaa aatggataaa tggctgtacg ccgatatcac
    32341 gcatttttcc cagttctggc attacctgaa cgaacaggat gaaacgcccg gatttgccga
    32401 tgacatgacc tgggatttta tcagcaacgt caatagcata acccgcaatg cgatgctcta
    32461 tgacgcgttg aaagcgatga agttcgccga tttcgccgtc tggtcagagg cgcgttttag
    32521 cgggatggtc aaaacggcgc tgacgctggc agtaacgaca atcttaaagg aattaacgcc
    32581 gtgaaaattg aattaacggt gaatgggctg aagatacagg cacagtacca gaacgaagag
    32641 attgaaaatg tacataaacc gttgctgcgt atgctggcgg ccttacagac ggtaaatccg
    32701 cagcggcgta cggtggtttt tctttgcgct ccgccgggga cgggtaaatc taccctaacc
    32761 accttctggg aatatctcgc gcaacaagac ccggagctgc ccgctattca gacgctcccg
    32821 atggatggtt ttcaccatta caatagctgg ctggacgcgc atcaattgcg ccccttcaaa
    32881 ggcgcaccag agacatttga cgttgcgaaa ctggcggaaa atctgcgcca ggtggtggaa
    32941 ggggattgta cgtggccgca gtacgatcga caaaagcatg atcctgttga agatgcgttg
    33001 cacgttaccg caccactcgt catcgtcgaa ggaaactggt tgttgctgga cgacgagaag
    33061 tggtgccagc ttgcgcaatt ttgtgatttc tcgatcttta tcaacgcgcc agctactgcg
    33121 ttgcgggagc gtctggtggg gcgcaagctg gcgggagggg tatcgctggc agatgcagaa
    33181 gccttttatg accgtaccga cgggccgaat gttcgccggg tgctggaaga gagtctgcct
    33241 gcaaatttga ccttaatgat gaccgctacg ggtgaatatc gtctggtgga ttaacgttcc
    33301 tgattacacc atcttccagt cgcaccacac gagagaaatg acgccgggta aatgcggcgt
    33361 catgactgat tgctaccacg ctcgtgcctc gctggcggca cttctccagc cagctttcaa
    33421 acacgctcaa ccaattttcg tcaaaatccc gactcggctc gtccagcagt aatagtggcg
    33481 gagagagtgc ttccaggcag gcgacggcga ccatccgtcg ctgcgcacta tgcagatcca
    33541 acgggtgcga gttggcagtg tctgctaact gacaacattg cagggcagca tgggtacgtt
    33601 gagtgatttc atccgccggg catttttgca gtttcaaacc aaatgcgatc tcgtcagcga
    33661 cggtgctgtg aaaaagttgg ttttccgctt cctgaaacag tacgccaacc tttgccgcgc
    33721 gctggcggtt tttcaggttt ttcatcgcct gttgttgcag cataaccgta ccggctgtgg
    33781 gcgtaagaag acctgccatt actcgtaaca gcgtcgattt ccctgcgcca ttgtcaccgg
    33841 tcagcgccaa ccattcacct tgtttgagtt gcagagaaat gtcgcaaaga caatccgttg
    33901 ccgcgccagg ccagcgataa gatatctgat ttaacgttaa catagtggct ggagcgctcc
    33961 gttttgcaat tgccacgctt gctggcagta gttcaggaaa ggtgtgcgat gacgttcaaa
    34021 aagaataatc agcgaaccac gttcaaacgc ccagtgttgt aagcgctgca acagcatttc
    34081 gctggcctgc ggcgtcaggc ggctaaaggc ttcatcaaga attaagagct ttggctgcat
    34141 cgcgatggcg caggcgatga ccacgcgctg tgtctctcca ccggagagtg ttgccgggtg
    34201 acgatggcgc aacggctggc actcggtcag agtcagggcg gcatcaatcc gcgccataat
    34261 ctctgcttca gcaagacaca gattttctgg cccaaaggca acttcttctt ccacgctaaa
    34321 ggtacagccg gagagctgca aatagggcga ttgctggacg agctggatgg tggcggattg
    34381 ttcattaagc gggagatgcc caatgggcgt accgaggagc gtccccgtgc ctgttatctc
    34441 tcctggcaga aagtccggat accagcccgc catcagctgt gccagcgtac ttttgccact
    34501 gccgttgtca ccaaagatgg cgaccatccc cggtttgaca tagtgaaagt cataacagaa
    34561 cggaggatgc gttgagtgtg tcgggcaata acggaactgt tctaacgtaa ccatatccac
    34621 gctccgaatt ctgtcagcat cagcaagatc atggtgtaac gcgctacgcg ttgtagggtg
    34681 ctatcggctg gtgcccacaa cgttgttcgt cggttgtgga tccgaaatgc ccgcatatcc
    34741 agcgccgcac cacggacagc cagatcgtta agcgcattgt gtgttagcgg aataattaac
    34801 gcgggcatcg cacgtaaacg ctgataccag ccctcatcca gcggcacgcc gcgggcacgc
    34861 tgcgcttcat gaataatcgc taattgccgt tttaactgct caacgaccaa taacggcccg
    34921 gcaaacagat aagcgacgcc tggcggcagg cgagaagcaa acagagcgcg aataaaacat
    34981 tgcacgggga cgtactgcat ccacaactgt gaggttgaaa caatcaccag aatccgcaac
    35041 cagagcgtaa tagcatgcga ccaccgttct ggcgagcgtg gcgtgccgct tagccattct
    35101 ggtagccagc caccgtgtac cagccacagc ccggctccca gcgagaacat cagccagacg
    35161 acatattttg cccgccgccg cgtggccttt agcgcaataa ggcagaaaaa ggtcgccgcg
    35221 ctatagatcg gcaaaatggt ctgcgcgggc aggataagcg tggtgcaggc cgccaatgcc
    35281 cacagtgtta atgaggtaaa tggatgcatt tagcgcacgg cagccattgc cggaaaatgg
    35341 cgcgtggtgc gaatcggcag ctggcgcagt aaaagccaga caatcaccgc tgtaagaatt
    35401 ttatcgacca ggttcgcgcc tatcaccgta atcgccaccg actccaccag gttttgcccc
    35461 atcgagtgca tccaggcaac gaacaaatcg gctccgctac cggtgacacc gccgaacagc
    35521 gcagtacgca gcggtacagc gacgaccgtt accgcaagcg taataatcac gccgctgaca
    35581 acgacttttg gcagggtgcg aaaccaaccc gctcgcgcca gccaaccggc gaccaggccg
    35641 atgaccatcg cgacgggtgc aaatgcggcg gcgatcgggt cggtcagcaa tccccagagt
    35701 aaattggtca gtaagccggt cagcattccg atcaccggac caagcagtac cgcgctaata
    35761 agtgtgccga tggagtcgag aaatataggt aatttgacca tgctggccag ttgcccgccg
    35821 atcatattga tggcgataga tataacgatc aacaccagtg cctgactgga aaagtggcta
    35881 cgcgccataa gaaaacctct ttgaaagtag ttatttaacg ggggaaatat gcaactcgtc
    35941 gtaaccagaa agcgcgcaag gttggcggct aaacgccagt tcctcaaggc taccgatcac
    36001 cagttcaagc gtggtccgca cggtacagcc gggcatcata tgaccgccaa gcatcgtgcc
    36061 gtgtggatcg gaaacgcaga gatgaagatg ctcaccactc tgttccagcg tgccgtttag
    36121 tgagatcact tcgaatgtac cgttcaggag cgtggtgcct tcttgcctgg catagcgcaa
    36181 ggcgacgtca gtcaggctac cggtacagcc tgcaatccag gcggcatgaa gttgatgctg
    36241 ctgtgcaaaa gcgtgtagct gggaaaaaac ttcctgcccg gggaggaggc gcagtgcgta
    36301 aaagcgtgcg gaggaagaat atgggtaggg caatgtcatc attggagtac tccgaaactc
    36361 ggacgtattg cggataatat cgacgctgca tcagtcatat tctgatataa gtcagggttt
    36421 gtgacggcac aacgaaaaga ttttgctacg cttattcgtg attctgcaaa gtggaataaa
    36481 ttctacaggg gcggggctga aaacgtgacg taagtcagat aattgctttc tttgactgga
    36541 caatcattcc ttttattcca cgtttcgctt atcctagctg aagcgtttca gtcgattaaa
    36601 tgttcgacaa ttaaccaatc agtcgcagtt tgcgacaggt aaggtttccc cggacgattt
    36661 gctggattac tctgtctggc aaatttgctc agggaaaacc ttgcaggaga tctatgaccg
    36721 tacgcgtagc gataaatggc ttcggtcgca tcgggcgtaa tgtggttcgt gctttgtatg
    36781 aatccggacg ccgggcggaa attaccgtgg tggcaatcaa cgaactggcg gatgctgcgg
    36841 gcatggcgca tttgttgaaa tatgacacca gccatggccg ttttgcatgg gaagtacgac
    36901 aggaacgcga tcaacttttt gttggtgatg acgccatccg cgtattgcat gaacgttcac
    36961 tgcaatcgct cccctggcgt gaacttggcg ttgatgtagt cctcgactgc accggcgtat
    37021 atggctcccg cgagcatggc gaagcgcata ttgccgccgg ggccaaaaaa gtgctctttt
    37081 cacatcctgg cagtaacgat ctcgacgcga ccgttgttta cggcgtcaat caggatcaac
    37141 ttcgtgcgga acaccgcatc gtttctaacg cttcctgtac cacgaattgc ataattcccg
    37201 tcatcaaatt gttagatgat gcgtacggta ttgagtccgg cactgtgacc acaattcact
    37261 ccgccatgca cgatcaacag gttattgatg cataccatcc tgacctgcgt cgcacccggg
    37321 cagccagcca gtcgatcatt ccggtcgata ctaaactggc cgccggtatc acacgatttt
    37381 ttccgcaatt taacgatcgc tttgaagcga ttgcggtacg tgtgccaacc ataaatgtga
    37441 cggcaatcga tttaagcgtg acggtgaaga aacctgtaaa agccaatgaa gtcaacctgt
    37501 tgctgcaaaa agcagcacaa ggtgcatttc atggtatagt tgactatacg gaattgccgt
    37561 tggtctctgt agattttaac cacgatccgc acagtgccat tgtcgatggc acccaaaccc
    37621 gggtcagtgg cgcacacctg atcaaaacgt tggtctggtg cgataacgaa tggggctttg
    37681 ctaaccgaat gctcgacacg acgttagcta tggctactgt tgctttcagg taagacgcaa
    37741 gcagcgtctg caaaactttt agaatcaacg agaggattca ccatgtctgt aattaagatg
    37801 accgatctgg atcttgctgg gaaacgtgta tttatccgtg cggatctgaa cgtaccagta
    37861 aaagacggga aagtaaccag cgacgcgcgt atccgtgctt ctctgccgac catcgaactg
    37921 gccctgaaac aaggcgcaaa agtgatggta acttcccacc tgggtcgtcc taccgaaggc
    37981 gagtacaacg aagaattctc tctgctgccg gttgttaact acctgaaaga caaactgtct
    38041 aacccggttc gtctggttaa agattacctc gacggcgttg acgttgctga aggtgaactg
    38101 gttgttctgg aaaacgttcg cttcaacaaa ggcgagaaga aagacgacga aaccctgtcc
    38161 aaaaaatacg ctgcactgtg tgacgtgttc gtaatggacg cattcggtac tgctcaccgc
    38221 gcgcaggctt ctactcacgg tatcggtaaa ttcgctgacg ttgcgtgcgc aggcccgctg
    38281 ctggcagctg aactggacgc gctgggtaaa gcactgaaag aacctgctcg cccgatggtg
    38341 gctatcgttg gtggttctaa agtatctacc aaactgaccg ttttggactc cctgtctaaa
    38401 atcgctgacc agctgattgt tggtggtggt atcgctaaca cctttatcgc ggcacaaggc
    38461 cacgatgtgg gtaaatccct gtacgaagct gacctggttg acgaagctaa acgtctgctg
    38521 accacctgca acatcccggt tccgtctgat gttcgcgtag caaccgagtt ctctgaaact
    38581 gcaccggcta ccctgaaatc tgttaacgat gtgaaagctg acgagcagat cctggatatc
    38641 ggtgatgctt ccgctcagga actggctgaa atcctgaaga atgcgaaaac cattctgtgg
    38701 aacggtccgg ttggcgtgtt cgaattcccg aacttccgca aaggtactga aatcgtggct
    38761 aacgctatcg cagacagcga agcgttctcc atcgctggcg gcggcgacac tctggcagca
    38821 atcgacctgt tcggcattgc tgacaaaatc tcctacatct ccactggcgg cggcgcattc
    38881 ctcgaattcg tggaaggtaa agtactgcct gcagtagcga tgctcgaaga gcgcgctaag
    38941 aagtaaaaaa tcacagggca gggaaacctg cccttgtttc agcgcgcttt tagagcacgc
    39001 attttttcat actctaaata attcgagttg caggaaggcg acaagagagt gaatccccag
    39061 gagcttacat aagtaagtga ctggggtgag cgaacgcaga cgcagcacat gcaacttgaa
    39121 gtatgatgag tataaggccc gacgatacag gacaagagac atgtctaaga tttttgattt
    39181 cgtaaaacct ggcgtaatca ctggtgatga cgtacagaaa gttttccagg tagcaaaaga
    39241 aaacaacttc gcactgccag cagtaaactg cgtcggtact gactccatca acgccgtact
    39301 ggaaactgct gctaaagtta aagcgccggt tatcgttcag ttctccaacg gtggtgcttc
    39361 ctttatcgct ggtaaaggcg tgaaatctga cgttccgcag ggtgctgcta tcctgggcgc
    39421 gatctctggt gcgcatcacg ttcaccagat ggctgaacat tatggtgttc cggttatcct
    39481 gcacactgac cactgcgcga agaaactgct gccgtggatc gacggtctgc tggacgcggg
    39541 tgaaaaacac ttcgcagcta ccggtaagcc gctgttctct tctcacatga tcgacctgtc
    39601 tgaagaatct ctgcaagaga acatcgaaat ctgctctaaa tacctggagc gtatgtccaa
    39661 aatcggcatg actctggaaa tcgaactggg ttgcaccggt ggtgaagaag acggcgtgga
    39721 caacagccac atggacgctt ctgcactgta cacccagccg gaagacgttg attacgcata
    39781 caccgagctg agcaaaatca gcccgcgttt caccatcgct gcgtccttcg gtaacgtaca
    39841 cggtgtttac aagccgggta acgtggttct gactccgacc atcctgcgtg attctcagga
    39901 atatgtttct aagaaacaca acctgccgca caacagcctg agcttcgtat tccacggtgg
    39961 ttccggttct actgctcagg aaatcaaaga ctccgtaagc tacggcgtag tgaaaatgaa
    40021 catcgatacc gatacccaat gggcaacctg ggaaggcgtt ctgaactact acaaagcgaa
    40081 cgaagcttat ctgcagggtc agctgggtaa cccgaaaggc gaagatcagc cgaacaagaa
    40141 atactacgat ccgcgcgtat ggctgcgtgc cggtcagact tcgatgatcg ctcgtctgga
    40201 aaaagcattc caggaactga acgcgatcga cgttctgtaa gatattcctt tctgcttatc
    40261 tcaaggcccg ctctgcgggt ctttttttcg ccaaaagcaa aattgcctga tgcgctacgc
    40321 ttatcaggcc tacacgtatc ctgcaatata ttgaatttgc aggtttttgt aggccggata
    40381 aggcgttcac gccgcatccg gcatgacaaa gcgcacattg atatctgtct gagccccgct
    40441 ctgcggggat tttttcgcca aaagcaaggt gattcagatg agaaatctgt gacctatttg
    40501 gcaaaattat gctttattgt ttacccttgt cagactgccc gtcataaggc ggcggagtgt
    40561 atttctccat tttgagtcag ttgaaaagga atattgaatg gaagatttga atgttgtcga
    40621 tagcataaac ggcgcgggaa gctggctggt agctaaccag gcgctgctgc taagttatgc
    40681 agtaaacatc gtggcggcac tcgcgatcat catcgttggt ttgattatcg cgcggatgat
    40741 ttccaacgcg gtgaatcgcc tgatgatctc ccgtaaaatc gatgccactg ttgctgattt
    40801 tctttctgca ttagtccgtt acggtattat cgcctttacg ctaatcgctg ccctgggacg
    40861 cgtgggtgta caaacagcgt cagtcattgc tgtactcggt gccgcaggct tagctgttgg
    40921 tctggctttg caggggtcac tttctaacct ggccgctggc gtgttacttg tcatgttccg
    40981 accgttccgt gccggagaat atgttgacct cggcggcgta gccggtactg tgctgagtgt
    41041 gcagattttc tccaccacca tgcgtactgc agacggtaaa attatcgtta ttccgaacgg
    41101 taaaattatt gccggaaata ttattaactt ctcccgcgag ccagttcgcc gtaacgaatt
    41161 tattattggc gtggcgtatg attccgatat cgatcaggtt aagcagatcc tgaccaatat
    41221 tatccagtct gaagatcgca ttttgaaaga tcgcgaaatg actgtgcgcc tgaacgaact
    41281 tggtgcatcg tcgattaatt tcgtggtccg cgtctggagc aacagcggcg atctgcaaaa
    41341 cgtgtactgg gatgtgctgg agcgtattaa acgtgaattt gatgccgccg gtatcagctt
    41401 cccgtacccg caaatggatg tgaactttaa gcgggtgaaa gaagacaaag ctgcgtaatc
    41461 aacgctgagg ccagataata ctctctggcc tctctcttat tagttattct gattgccaat
    41521 taatattatc aatttccgct aataacaatc ccgcgatata gtctctgcat cagatactta
    41581 attcggaata tccaacgtgt tttcttatta ctttcaaggt cttgatcttg gggcagctat
    41641 gattctgccg ctcggcccac aaaatgcttt tgtgatgaat cagggcattc gtcgtcagta
    41701 ccacattatg attgccttac tttgcgctat cagcgatttg gtcctgattt gcgcggggat
    41761 ttttggtggt agcgcgttat tgatgcagtc gccgtggttg ctggcgctgg tcacctgggg
    41821 cggcgtagtc ttcttgctct ggtatggttt tggcgctttt aaaacagcga tgagtagtaa
    41881 tattgagtta gctagtgccg aagtcctgaa gcaaggcaga tggaaaatta tcgccaccat
    41941 gttggcagtg acctggctga atccgcatgt ttacctggat acttttgttg tactgggcag
    42001 ccttggcggg cagcttgacg tcgaaccaaa acgttggttt gcgctcggga caattagcgc
    42061 ctctttcctg tggttctttg gtctggctat tctcgcagcc tggctggcac cgcgcctgcg
    42121 cacggcaaaa tcacagcgca ttatcaatct ggtagtggga tgtgtcatgt ggtttatagc
    42181 cttgcagctg gcgagagacg gtattgctca tgcacaagcc ttgttcagtt aggcgctatc
    42241 tgatggaaaa ataaaacaga ggcgctaagc ttgcctccag aggtcctgaa ttttgggcaa
    42301 gtcaattaac acggagagac taacgtgaag ttcaaagtta tcgccctagc ggcattaatg
    42361 ggtattagcg ggatggcagc gcaggctaac gaattgccgg atggaccgca tattgtcacc
    42421 tccggtacgg caagtgtgga tgtggtgcca gacattgcca ctcttgcgat tgaagttaac
    42481 gtggccgcga aggatgccgc tacagccaag aaacaggcag atgagcgcgt cgcacaatac
    42541 atttccttcc ttgaactcaa tcagatcgcg aaaaaagata tcagctcagc gaatttacgc
    42601 acccagccag attatgatta tcaggatggt aaaagtatcc tgaaaggcta ccgcgctgtg
    42661 agaacggtgg aagtcacgct ccgtcagtta gacaaactga attccttgct ggatggcgcg
    42721 ctgaaggcgg gtcttaacga aattcgttca gtgtcgctgg gcgtggcgca gccggatgcc
    42781 tataaagaca aagcgcgtaa ggcagcgatt gataacgcga ttcatcaggc gcaggaactg
    42841 gcgaacggct ttcatcgtaa actggggccg gtatatagcg tgcgctacca tgtttccaac
    42901 tatcagccca gcccaatggt gcggatgatg aaagccgatg ccgcgccggt gtctgcccag
    42961 gaaacttacg agcaggccgc tattcagttt gatgatcagg tcgatgtggt cttccagtta
    43021 gaacctgtgg atcaacaacc cgctaaaaca cctgcagcac aataatactt gtagcccgac
    43081 gtattcgtcg ggctaatgcc tttacccgat attgcctcct ttttctgtaa ttttctgtaa
    43141 aaccatgacg taatcaccta tctttgctcg cggtttcatc aaggataggc tgactgaaac
    43201 ggttaagcgg acaaggatgc gggg
//
LOCUS       DAJVUJ010000010        42389 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.010, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000010 DAJVUJ010000000
VERSION     DAJVUJ010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42389
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.010"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            123..662
                     /locus_tag="OQQ48_000579"
     CDS             123..662
                     /locus_tag="OQQ48_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HCS1951458.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQVAANKRAATAAANKKARQDKLDAEASADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(757..2334)
                     /gene="guaA"
                     /locus_tag="OQQ48_000580"
     CDS             complement(757..2334)
                     /gene="guaA"
                     /locus_tag="OQQ48_000580"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCS1951459.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMHMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYNKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2403..3869)
                     /gene="guaB"
                     /locus_tag="OQQ48_000581"
     CDS             complement(2403..3869)
                     /gene="guaB"
                     /locus_tag="OQQ48_000581"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCS1951460.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4031..5401
                     /gene="xseA"
                     /locus_tag="OQQ48_000582"
     CDS             4031..5401
                     /gene="xseA"
                     /locus_tag="OQQ48_000582"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCS1951461.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRTGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRVQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(5398..5613)
                     /locus_tag="OQQ48_000583"
     CDS             complement(5398..5613)
                     /locus_tag="OQQ48_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCS1951462.1"
                     /translation="MELHCPQCQHVLDQDNGHARCPSCGEIIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(5682..7154)
                     /gene="der"
                     /locus_tag="OQQ48_000584"
     CDS             complement(5682..7154)
                     /gene="der"
                     /locus_tag="OQQ48_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCS1951463.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEVEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(7272..8450)
                     /gene="bamB"
                     /locus_tag="OQQ48_000585"
     CDS             complement(7272..8450)
                     /gene="bamB"
                     /locus_tag="OQQ48_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HCS1951464.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYD
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(8461..9081)
                     /gene="yfgM"
                     /locus_tag="OQQ48_000586"
     CDS             complement(8461..9081)
                     /gene="yfgM"
                     /locus_tag="OQQ48_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HCS1951465.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(9099..10373)
                     /gene="hisS"
                     /locus_tag="OQQ48_000587"
     CDS             complement(9099..10373)
                     /gene="hisS"
                     /locus_tag="OQQ48_000587"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCS1951466.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDLNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPIVDIYLV
                     ASGADTQSAAMALAERLRDELQGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     TNGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(10484..11602)
                     /gene="ispG"
                     /locus_tag="OQQ48_000588"
                     /gene_synonym="gcpE"
     CDS             complement(10484..11602)
                     /gene="ispG"
                     /locus_tag="OQQ48_000588"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HCS1951467.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(11629..12642)
                     /gene="rodZ"
                     /locus_tag="OQQ48_000589"
     CDS             complement(11629..12642)
                     /gene="rodZ"
                     /locus_tag="OQQ48_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HCS1951468.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLLGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPAPTAATTPDGAAPLPTDQAGVTTPVADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(12927..14081)
                     /gene="trmG/rlmN"
                     /locus_tag="OQQ48_000590"
     CDS             complement(12927..14081)
                     /gene="trmG/rlmN"
                     /locus_tag="OQQ48_000590"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HCS1951469.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(14231..14662)
                     /gene="ndk"
                     /locus_tag="OQQ48_000591"
     CDS             complement(14231..14662)
                     /gene="ndk"
                     /locus_tag="OQQ48_000591"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCS1951470.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(14811..17123)
                     /gene="pbpC"
                     /locus_tag="OQQ48_000592"
     CDS             complement(14811..17123)
                     /gene="pbpC"
                     /locus_tag="OQQ48_000592"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HCS1951471.1"
                     /translation="MLRLLTKRGCWITLAAVPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKILQLWRALQLEWHLSKREILILY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAVQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLEWPLMSSGAAWIIRRIMADEAQPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDCTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTSCP
                     PYGHYAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(17124..22085)
                     /gene="yfhM"
                     /locus_tag="OQQ48_000593"
     CDS             complement(17124..22085)
                     /gene="yfhM"
                     /locus_tag="OQQ48_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HCS1951472.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSVPERQKLAQQSAGKVLTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPDQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPNDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPANALPGLRPQFALK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPSDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPLVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGIALKTMGDATRGEEAIVLALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQYTLLNTLSQQAFGERWLSTQESNALFLAAP
                     TIQDLPGKWQAQTSFSAEPLTGEKTLNSNLNSDQLATLQVRNSGDQPLWLRMDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGDEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            22285..23130
                     /gene="sseA"
                     /locus_tag="OQQ48_000594"
     CDS             22285..23130
                     /gene="sseA"
                     /locus_tag="OQQ48_000594"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HCS1951473.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPKTFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            complement(23623..24399)
                     /gene="sseB"
                     /locus_tag="OQQ48_000595"
     CDS             complement(23623..24399)
                     /gene="sseB"
                     /locus_tag="OQQ48_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HCS1951474.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLFIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIVPFYERRWGGFLRDFKQNRII"
     gene            complement(24541..25824)
                     /gene="pepB"
                     /locus_tag="OQQ48_000596"
     CDS             complement(24541..25824)
                     /gene="pepB"
                     /locus_tag="OQQ48_000596"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HCS1951475.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRASERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(26002..26202)
                     /gene="iscX"
                     /locus_tag="OQQ48_000597"
     CDS             complement(26002..26202)
                     /gene="iscX"
                     /locus_tag="OQQ48_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HCS1951476.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(26214..26549)
                     /gene="fdx"
                     /locus_tag="OQQ48_000598"
     CDS             complement(26214..26549)
                     /gene="fdx"
                     /locus_tag="OQQ48_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HCS1951477.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(26551..28401)
                     /gene="hscA"
                     /locus_tag="OQQ48_000599"
     CDS             complement(26551..28401)
                     /gene="hscA"
                     /locus_tag="OQQ48_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HCS1951478.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAALRMDQSVRRALKGHSVDEV"
     gene            complement(28418..28933)
                     /gene="hscB"
                     /locus_tag="OQQ48_000600"
     CDS             complement(28418..28933)
                     /gene="hscB"
                     /locus_tag="OQQ48_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HCS1951479.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNETWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(29029..29352)
                     /gene="iscA"
                     /locus_tag="OQQ48_000601"
     CDS             complement(29029..29352)
                     /gene="iscA"
                     /locus_tag="OQQ48_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HCS1951480.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(29369..29755)
                     /gene="iscU"
                     /locus_tag="OQQ48_000602"
     CDS             complement(29369..29755)
                     /gene="iscU"
                     /locus_tag="OQQ48_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HCS1951481.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLVEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(29783..30997)
                     /gene="iscS"
                     /locus_tag="OQQ48_000603"
     CDS             complement(29783..30997)
                     /gene="iscS"
                     /locus_tag="OQQ48_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCS1951482.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(31109..31597)
                     /gene="iscR"
                     /locus_tag="OQQ48_000604"
     CDS             complement(31109..31597)
                     /gene="iscR"
                     /locus_tag="OQQ48_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HCS1951483.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(31867..32607)
                     /gene="trmJ"
                     /locus_tag="OQQ48_000605"
     CDS             complement(31867..32607)
                     /gene="trmJ"
                     /locus_tag="OQQ48_000605"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HCS1951484.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            32726..33529
                     /gene="suhB"
                     /locus_tag="OQQ48_000606"
     CDS             32726..33529
                     /gene="suhB"
                     /locus_tag="OQQ48_000606"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HCS1951485.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            33674..34528
                     /locus_tag="OQQ48_000607"
     CDS             33674..34528
                     /locus_tag="OQQ48_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCS1951486.1"
                     /translation="MALPVNKRVLKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKDTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQDDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKETKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            34719..35999
                     /gene="csiE"
                     /locus_tag="OQQ48_000608"
     CDS             34719..35999
                     /gene="csiE"
                     /locus_tag="OQQ48_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HCS1951487.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(35991..37130)
                     /locus_tag="OQQ48_000609"
     CDS             complement(35991..37130)
                     /locus_tag="OQQ48_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HCS1951488.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLAMMTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYQVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVLQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGAFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(37290..38180)
                     /gene="hcaR"
                     /locus_tag="OQQ48_000610"
     CDS             complement(37290..38180)
                     /gene="hcaR"
                     /locus_tag="OQQ48_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HCS1951489.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKTVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKARFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            38316..39677
                     /locus_tag="OQQ48_000611"
     CDS             38316..39677
                     /locus_tag="OQQ48_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HCS1951490.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKSPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            39674..40192
                     /locus_tag="OQQ48_000612"
     CDS             39674..40192
                     /locus_tag="OQQ48_000612"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HCS1951491.1"
                     /translation="MSAQVSLELHLRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYQAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            40192..40512
                     /locus_tag="OQQ48_000613"
     CDS             40192..40512
                     /locus_tag="OQQ48_000613"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HCS1951492.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGSDIFIDLP
                     EAQP"
     gene            40509..41321
                     /gene="hcaB"
                     /locus_tag="OQQ48_000614"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             40509..41321
                     /gene="hcaB"
                     /locus_tag="OQQ48_000614"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HCS1951493.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACTPALIASEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            41331..42348
                     /locus_tag="OQQ48_000615"
                     /pseudo
     CDS             41331..42348
                     /locus_tag="OQQ48_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
BASE COUNT        10023 a        11716 c        10641 g        10009 t
ORIGIN      
        1 taagttggca gcattaccag tttacgacag gtttagccgt tgttattacc acaacgtata
       61 acgaaacgcg tattcagcca aatactaaat gtcctgaaaa gagctaataa tcaggaggag
      121 tcatgaagaa agtttttctt tgcgccatct tggcctcctt aagctatccg gctatcgcct
      181 catcattgca ggatcaactc tctgctgtag cagaagcgga acagcaaggt aaaaatgaag
      241 agcaaaggca gcatgacgaa tgggtcgcgg agcgcaacag ggaaatccag caagagaagc
      301 aacgtcgcgc aaatgcccag gtcgccgcta acaaaagagc ggcaacggca gcagcaaata
      361 agaaagctcg tcaggataaa ctggacgccg aagcctctgc ggacaaaaaa cgcgatcaaa
      421 gttatgaaga tgagctacgc agcttagaga ttcagaaaca aaaactggcg ctggcaaaag
      481 aagaagcccg cgttaagcga gaaaacgaat ttatcgatca ggaactgaag cacaaagctg
      541 cgcaaaccga tgtggtgcaa tctgaagctg acgccaacag aaatatgact gaaggcggtc
      601 gcgatctgat gaaaagcgtg ggcaaagcag aagagaacaa atcggacagc tggtttaatt
      661 aatcgatgtt agtaacttca agcctatgat tcttgaagat aaaaaaccct ctgtagtaac
      721 agagggtttt gttcattcat agtgcaggga ttaaaatcat tcccactcaa tggtagctgg
      781 cggcttgccg ctgatgtcat acaccacgcg ggaaataccg ttcacttcat tgataatgcg
      841 gttggaaacg cgaccgagga aatcgtacgg cagatgcgcc cagtgtgcgg tcataaagtc
      901 gatggtttcg acagcacgca gagagacaac ccagtcatac ttacgaccat cgcccattac
      961 gccaacggaa cgtaccggca ggaacacagt gaacgcctgg ctgactttgt tgtacaggtc
     1021 cgctttacgc agttcttcaa tgaagatggc gtcagcacgg cgcagcaggt cacagtactc
     1081 tttcttcact tcacccagca cacgaacgcc aaggcctggt cccgggaacg ggtgacggta
     1141 cagcatgtcg tacggcaggc ccagctccag accaatctta cgcacttcgt ctttgaacag
     1201 ctctttcagc ggttcaacca ggcccatctt catctctttc ggcaggccgc ccacgttgtg
     1261 gtgagatttg atgacgtgtg ctttaccggt tgcagaagcc gcagattcga taacgtcagg
     1321 gtagatggtg ccctgcgcca gccacttcac gtcttccagt ttcagcgctt cttcatcgaa
     1381 cacttcaacg aaaacgcgcc cgatgatttt acgttttgct tccggatcgt tttcgccagc
     1441 cagcgctgac aggaagcgat cttctgccgg tacgtgaaca atgttcagac caaagtgatc
     1501 gccaaacata tccagaacct gctctgcttc gttgagacgc agcaggccgt tgtcgacgaa
     1561 tacgcaagtc agatttttac cgatagcgcg gtgcagcagc attgcggtta cggaggaatc
     1621 cacaccacca gagaggccga ggatgacttt atcgtcgcct acctgctcgc ggatgcgagc
     1681 tacagcatcg tcgataattt tcgctggcgt ccacagggct tcacactggc agatatcacg
     1741 cacaaaacgc tccagcatgt gcataccctg gcgggtgtga gtcacttccg ggtggaactg
     1801 tacgccatag aagcgttttt cttcgttagc cataatggca aacgggcagc tttcggtgct
     1861 ggctacggtg acgaagtcgg acgggatagc ggtaacttta tcgccgtggc tcatccagac
     1921 atcgagcagc ggtttaccgt ctgcggtcag cgcatcttcg ataccgcgaa ccagtgcgct
     1981 gtcgtttacg acttcaacct gcgcgtagcc aaattcacgt tcgttagagg cttcaacgtg
     2041 accgcccaac tgcattgcca tggtctgcat gccatagcaa acgccgaata ccggtacgcc
     2101 tgcttcaaag acatactgcg gcgcacgcgg actgttttct tcagtagtac tttccgggcc
     2161 gccggaaaga ataatgccgc ttggattgaa gtcacgaatt tgtgcttctg tcacatccca
     2221 cgcccacagt tcgcagtaaa cacccagctc acgcacgcgg cgcgcaacca gttgagtgta
     2281 ctgagaaccg aagtccagaa tgaggatgcg atgcttatga atgttttccg tcattgacgc
     2341 ttattccgag gcaagtgaaa cagataatat aaatcgcccg acatgaagtc gggcgaagag
     2401 aatcaggagc ccagacggta gttcggggac tctttagtaa tggtcacgtc gtgaacgtgg
     2461 ctttcctgaa tgcccgcacc gctgatacgt acaaactccg ctttagtacg cagttcgtcg
     2521 atagtaccac agccggtcag gcccatacag gagcgcaggc cgcccatctg ctggtgaatg
     2581 atctctttca ggcgaccttt ataggctacg cgaccttcga taccttccgg cactagtttg
     2641 tcggcagcgt tgtcactctg gaagtaacgg tcagaagaac ctttggacat cgcacccaga
     2701 gagcccatac cacggtaaga tttgtaagaa cggccctggt agagttcaat ttcacccgga
     2761 gattcttcag tacccgccag catggaaccc accattaccg cgcttgcgcc agcggcgata
     2821 gctttggcga tgtcgccgga gaagcgaata ccaccatctg cgataaccgg aatacctgtg
     2881 ccttccagcg cttcaactgc gtcagcaacg gcagtgatct gcggaacacc tacgccagta
     2941 acgatacgag tcgtacagat agaaccaggg ccgataccga ctttaaccgc gctgcaacca
     3001 gcttctgcca aagcgcgtgc acctgcagct gttgccacgt tgccgccgat gatttgcaga
     3061 tccggatatt tagcacgggt ttcacggata cgctgcaaaa cgccttctga gtggccgtgg
     3121 gaggagtcga tcagcagaac gtcaacgcca gcggcaacca gcgcgtcaac acgctcttcg
     3181 ttacccgcac ctgcgccaac tgcagcacca acacgcagac ggccttgctc gtctttacag
     3241 gcgttcggtt tacgttccgc tttctggaag tctttcacgg tgatcatgcc gatcaggtgg
     3301 aattcgtcat caaccaccag cgctttttca acgcgttttt cgtgcatttt tgccagcacc
     3361 acttcacggg cttcaccttc acgcacggtg accagacgct ctttcggcgt catgtaaacg
     3421 ctaaccggct ggttcaggtc ggtaacaaaa cgcacgtcac gaccggtgat aatacccacc
     3481 agttcgtttt cttcggtaac aaccggatag cccgcaaaac cgttacgctc ggtcagttct
     3541 ttcacttcgc gcagcgtcgt ggttggcaga acagtctgcg gatcagtcac cacaccagat
     3601 tcgtgttttt tcacacggcg aacttcttct gcctggcgtt caatggacat gtttttgtgg
     3661 ataaagccga taccgccttc ctgagccaga gcaatagcca ggcgcgcttc cgttacggta
     3721 tccattgctg cggaaagcat agggatattc agacgaatag ttttcgtcag ctgggtgctg
     3781 aggtcagcag tattcggcag aacggtagaa tgagcaggaa cgaggagaac gtcgtcaaac
     3841 gtcagagctt ctttagcgat acgtagcatg ggcaatatct cgaccagagt ggttaataaa
     3901 tattgccgcg gcattataca gagcgtaacc gattgcatct accccttttt gcaaaaaatg
     3961 cttgctatcc ccgaagggcg ggttactatc gactgaataa cctgctgatt tagaatttga
     4021 tctcgctcac atgttacctt ctcaatcccc tgcaattttt accgttagtc gcctgaatca
     4081 aacggttcgt ctgctgcttg agcatgagat gggacaggtt tggatcagcg gcgaaatctc
     4141 taatttcaca caaccggctt ccggtcactg gtactttaca ctcaaagacg acaccgccca
     4201 ggtacgctgc gcgatgttcc gcaacagcaa ccgccgggtg accttccgtc cacagcatgg
     4261 acaacaagtt ttagttcgcg ccaatattac gctctacgag ccgcgcggtg actaccagat
     4321 aatcgttgag agtatgcagc cggccggtga agggctgctg caacagaagt acgaacagct
     4381 caaagcgaag ttgcaggctg aaggtttgtt cgatcagcaa tacaaaaaac cacttccctc
     4441 ccctgcgcat tgcgttggtg tgatcacctc aaaaaccggt gctgcgctac atgatatttt
     4501 gcatgtgtta aaacgtcgcg atccttctct accggtgatc atctacccca ccgccgttca
     4561 gggcgatgac gcaccggggc aaattgttcg cgccattgaa ctggcgaatc agcgcaatga
     4621 gtgcgacgtg ttgatcgttg ggcgcggcgg cggttcgctg gaagatttat ggagttttaa
     4681 cgacgaacgc gtagcgcggg cgatttttgc cagccgcatt ccggtcgtca gcgccgtcgg
     4741 gcatgagacg gatgtgacca ttgccgattt tgttgccgat ctgcgtgcgc caacgccgtc
     4801 tgccgccgct gaagtagtga gccgtaatca gcaagagtta ctgcgccagg tgcaatcgac
     4861 ccgtcaacgg ctggagatgg cgatggatta ttatctcgcc aaccgcacgc gtcgctttac
     4921 gcagatccat caccgattac agcaacagca tccgcagctc cggctggcac gccagcaaac
     4981 catgcttgag cgcctgcaaa agcggatgag ctttgcgctg gaaaatcaac ttaagcgtac
     5041 cgggcaacag cagcagcggt taacacagcg gctgaatcag caaaatccac agccgaagat
     5101 tcatcgcgcg caaacgcgca ttcagcaact ggaatatcgt ttagcagaaa ccctgcgcgt
     5161 acagcttagc gccacgcgtg aacgtttcgg taatgcagta acgcacctcg aagccgtaag
     5221 cccactgtca acgctggcgc gtggatacag cgttactact gctactgacg gcaatgtact
     5281 gaaaaaagtg aagcaagtta aagcgggtga aatgctaacc acacgtctgg aagacggctg
     5341 gatagaaagt gaagttaaaa acatccagcc agtaaaaaaa tcgcgtaaaa aggtgcatta
     5401 agccagcacg aattcaacgc gttttttcga tatcaagcca tgcccgtgct gacagaagta
     5461 atctactgca ccgcaggctt ttaacacctg caacggttga tggcagtctg gacaaagagc
     5521 tttcatttca ataatttccc cgcaggatgg gcaacgcgca tggccgttat cctgatcaag
     5581 cacatgctga cattgtggac aatgcagttc catatgaatt cctccggtag ccatccctct
     5641 acattcatag agggaatggc agataaaata cttacggata attatttatt tttcttgatg
     5701 tgcttcatca gacgcttacg tttacgcatc tgggttggcg tcagggtgtt acgcttgttc
     5761 gcatacgggt tttccccttc tttgaactga atacgaatcg gcgatcccat tacgtccagc
     5821 gatttgcgga agtagttcat caagtagcgc ttgtaggaat caggcaggtc tttcacctga
     5881 ttaccgtgaa tcaccacaat cggcgggtta taaccaccag cgtgggcata tttcagcttc
     5941 acacgacgac cgcgtaccag cggcggttgg tgatcttcaa cagccatcgt catgatgcgc
     6001 gtcagcatag aggtccccac acgacgggtg gagctgtcat acgcttcacg tactgattca
     6061 aacaagttac caacaccact gccgtgcaag gcagagataa agtgcacacg agcaaaatcg
     6121 ataaagccca gacggaagtc cagcgtttct ttcacctgct ctttcacttc ctggctcagg
     6181 ccatcccact tattcaccac aatgacaagt gagcgcccac tattgagaat aaagcccagc
     6241 agcgagagat cctgatcgga aataccttcg cgcgcatcaa tcactaacat caccacgttg
     6301 gcatcttcaa tggcctgcaa cgttttgatt acggagaatt tctctacagc atcggtgatt
     6361 ttgccgcgtt tacgtacgcc agcggtgtca atgagcacat actcacgtcc atcgcgttcc
     6421 attggaatgt agatactgtc acgcgtcgtg ccaggcatgt cataaacaac aacgcgctct
     6481 tcaccaagaa tacggttagt gagtgtagac ttacctacgt tcggacgacc cacaatcgcc
     6541 agtttgatcg gcagactttg cgggtcgaag tcgtcttcct cttcttcttc gccgttctct
     6601 tccacttcaa attgcgccca gtattcagcg tcttcgtcga cttcctcttg cggtgcgaga
     6661 tcttccatcc acggcagcag cacatgctcc agcagactta atacgccacg accgtgagac
     6721 gcggcgatcg ggtagatttc acctaagcca agcgagtaga aatcaaccac tgcctgatcg
     6781 ggatccagac cgtcagtttt gtttgccacc aggaaggtcg gtttttcacg ggagcgcaga
     6841 tgtttggcaa tcgcttcatc tgccggcatc aggcccgcgc gcgcatccac cataaacagt
     6901 acgacgtccg cttcttcaat cgccagcagc gactgttccg ccatgcgggt ttctacaccg
     6961 tcttctgtgc catcaatccc gccggtatca atacagataa actcacggcc ttcaatttcc
     7021 gcacgaccgt acttacggtc acgagtcaga cccgggaaat ccgcaaccag cgcatctcgg
     7081 gtgcgagtta gacggttaaa taacgtggat tttcctacgt tagggcgccc gacaagcgcg
     7141 accacaggta ccatgtttaa agcctcattt ttataaatca tcagacaacg cacgctatat
     7201 tcgcgtcgtt gttaaaaaca ggaaaacggc ccctgtccag gagccgtttt caaagtgaac
     7261 gacagagacg attaacgtgt aatagagtac acggttccgt cttttgcctg gatcagcagt
     7321 ttgccgtcag cggcaaccgg ttcagtctgg aaaccggaac tatcaacttt ttgctgggca
     7381 acgaaacgac catcttcgac attaatccag tgcagataac cttcactgtc accgaccacc
     7441 aggttgccat catacagcac cggagaagtc agcaggcgat gcaacagatc gctttgtgtc
     7501 cacagcgtaa cgccgccatc aatggtcaac gccatcaccc ggtcattttg atcgaccaga
     7561 tagatgcgat tgccgtcgac gatgaaatca ttcaccgaac ccagttcgcg tttccacata
     7621 atctgaccac tgcgcagatc aagcgccgtc aggttaccat tataggccag cgcgaaaaca
     7681 acgccgttaa caacgacggg agtcgtgtca acatcgctca gacggtcaat ttcggtagaa
     7741 ccggtcgcct gggaaatacg ctgctgccaa atcatctggc cctgttccat cagcactgcg
     7801 ctgacgcgac cattatcgcc ccccacgacg gccgcaccaa aagccgttgc cggcgcagac
     7861 tcgccacgca aagagagcga aggcatatcg aggttaactg tccatttgac agcgccgtca
     7921 gcttcgttca gcgcttgtaa ctgaccgtta ctggtgtgga ttaacaccag accgtcgctg
     7981 accaccgggc gcgaaagtgc ttcacccgcg actttagttt gccatgccac agtaccatcg
     8041 ctggtattca gcgcgtaaac ctgcgccttt tcgctgccaa tgtagacatg cccaccagac
     8101 acggtcacac cgccagaaag taatgcaggc tctttagaga accagccatc tttctcggcc
     8161 aggctgacag accagatttc tttgccatca tccgcattca gcgcttttac caaaccagcg
     8221 cggtccgctg catagacaac gttgtccgcc agtgccggat gaagattgga atagaagttg
     8281 ccaatgccgc taccaacgga agtactccac gccgtggtcg gcgtaaactg gttttcaacg
     8341 gttggcaatg gggacatctt taccacatct tcttcgctgt taaacagcga acagccgctt
     8401 aaaagggtaa cggaaagcag tcctggcagc agtaatttac gcaattgcat cgggtccctc
     8461 tcagatggac aaattattaa ttttcatctg catcatttcg ctcagtgccg gagtaacatc
     8521 gcttttcacg cctgcttccc atgcactacg cgcaccttgc ttatcacctt tgctcagcaa
     8581 tgcttcacca cgcaggtcgg caacaatggc agcccaccct tcacctttga tggtatcaag
     8641 ggttttcagc gcggcatcag cctgcttgag ctgtacctga acgcgagcaa gacgcagatt
     8701 tatcacggct ttgagatttt catcgctcgt gtctgccagc ccctgttgta actgggcggc
     8761 agctttctct agttcatttt tgtcaacaaa ttgctgcgca agttccaaag aagccagcgc
     8821 accataagta tttttatttt cagcagcaaa tttttccgcc gccgggatgc tatccggttt
     8881 gccttcgctc actgcggtaa ccgcattttg ataggcaaga gaagcggagc gtgcagaatc
     8941 aacctgatgg ctgttccagt agcgccagcc aatcagtgcg ccaacgccca aaatcacccc
     9001 aacagccagt gctttgccat tttcagcaaa aaagcgttta accgcttcta cctggtcgtt
     9061 ttcgttctcg taaatttcca cgctgtcctt ctccttcctt aacccagtaa cgtgcgcaaa
     9121 tgcgcggcta cgctatcctg cgcaactgcc gtttgctcac cagagcgcaa atccttcact
     9181 actgctgtgc cgttagtcac ttcagactca cccagcacca cagcaacgcg ggcaccccat
     9241 ttatcagcac gggcaaactg tttcttaaag ttgccgccgc cgtggttggt catcaatttc
     9301 acgccctgta attcatcacg cagacgctca gctaatgcca tagccgcaga ttgtgtatca
     9361 gcacctgaag ccaccaggta tatatcgaca ataggatcgg ctttaaattc cggattaacg
     9421 gcctgtacta acaatacaag acgttcgagg cccatcgcaa aaccgacagc cggtgttgca
     9481 cgaccgccca gttgttccac aagaccgtca taacgaccgc ctgcacacac ggtgccctgg
     9541 gagccgagac tgttagtcac ccactcgaaa acggtacggt tatagtaatc cagaccacgc
     9601 accagacgct ggtttacggt gtaagcgatc cccgcgctct caagcagttt gcacagaccg
     9661 gcaaaatgct cacgagattc ctcgtccaga taatcaccta atgccggagc gtcgttgaga
     9721 agcgcctgca cttccggatt ttttgaatcc agcacgcgca gcgggttagt gtacatgcgg
     9781 cgtttgcagt cttcgtccag cttttcttta tgctgctcaa ggaatgccac cagcgcatcg
     9841 cggtaattgg cgcgtgcttc cagcgaaccg atagaattca gctcaagagt tacgtgttcg
     9901 gaaataccca gcgcgcgcca ccagcgggcg gtgagcataa tcagttcagc gtcgatatcc
     9961 ggaccttgca gaccgaaaac ttcgcagccc aactgatgga actgacgata acgccctttc
    10021 tgcggacgct cgtgacggaa catcggcccg atataccaca gacgctgttc ctgattgtac
    10081 agaagaccat gctcgatgcc ggcgcgtaca cagcccgccg tcccttcagg gcgcagagtc
    10141 aggctgtcgc cattgagatc ctcaaaggtg tacatctctt tttcaaccac gtcggtgact
    10201 tcaccaatcg cacgtttgaa tagcggggtc tgctctacaa tcggcaagcg gatttcactg
    10261 taaccgtagc tgccgagcac gtttttcagt gtgccttcaa tgcgctgcca gatggccgtt
    10321 tcgccaggca ggtaatcgtt catgccgcga atggcttgaa tgttttttgc cacgtttatt
    10381 ctctttctga atataaaaat gaaccctcaa cgcttccctc aatgtttggg gagccatgcg
    10441 ggttcaatca tacacgggaa gcgaggcgct tcccatcacg ttattatttt tcaacctgct
    10501 gaacgtcaat tcgacgcgct tcatccagct gactggcttt cgcacgaatg cgtgcttcca
    10561 gctggtcgat catatcgttg ttgtccagac ggtctttgcg cacgccatct tcatagaggc
    10621 cgcttttctt gttgccgccg gtgacgccga gtgtagaaac cagcgcctca cctgggccat
    10681 tcaccacgca gccgataata gaaacgtcca tcggagtgat gatatcttcc aggcgttgct
    10741 ccagcgcgtt aaccgtaccg ataacatcaa attcctgacg cgaacaggtc gggcaggcga
    10801 tgaagttgat ccctcgcgaa cggatacgca gcgatttcaa aatatcgaaa ccgactttga
    10861 tctcttcgac cggatcggcc gccagcgata cgcgcagcgt gtcgccgatg ccttcagaca
    10921 gcagcagacc taaaccaatg gcggatttta ctgccccgct gcgtgcacca ccggcttcgg
    10981 tgatccccag atgcagcggc tgatcgatct gttttgccag caaacgataa gactcaacag
    11041 cgaggaagac gtcagacgct ttcacgctga ctttgaactg atcgaagttc aggcgatcga
    11101 gatgatcaac atgacgcatg gcggattcca gcaacgcctg cggcgtcggt tcgccatact
    11161 tttcttgcag atctttttcc agcgatccgg cgttaacgcc aatacggatc ggaatgtttt
    11221 tatcgcgcgc acagtcaacc accatgcgaa tacgctcttc attaccgata ttgccagggt
    11281 taatacgcag acaatcgacg ccgtattccg ctactttcag cgcaatgcga tagtcgaagt
    11341 ggatgtcagc caccagcggc acgttaaccc gctgtttgat gagtttgaac gcttctgccg
    11401 cgtccatcgt cggtacggag acacggacga tatcagcgcc aacgcgttcc agcgccttga
    11461 tttgattgac cgttgcttcg acatctgtcg tacgcgtatt ggtcatggac tgtacggcga
    11521 tgggagcacc atcgccaatc ggcacattcc caacgtaaat acgtgttgat tttctacgtt
    11581 gaattggagc ctggttatgc atgaaaaatc tcccgcgtta cccgtctgtt actgcgccgg
    11641 tgattgttcg gcattgaggg tcagacgcgc aacctggtta gttctgataa aacgactcag
    11701 atcgacaggt ttcccttgat actggatctg tactgcggct ggcgcaccaa ttttcagttt
    11761 gtacggtgcc tggccggtta agttcaaatt accgtcttta cgctgcatac cgctaaacaa
    11821 ttttttaccg gtagcatcag tgacctccag ccagcaatcg gcagtaaagt tcatcaccag
    11881 cgcattcgga tcagccaccg gcgtggtcac gccagcctga tcggttggca acggcgcagc
    11941 accatctggc gttgttgctg ccgttggtgc cggggtcgcg gcggtatcaa catttgcctg
    12001 cgaaggcgaa acaaccgcat tctgttgcgg atcaacagct ggtgctggcg cagttacggc
    12061 aggtgtttgc gtgttggttg cggtagtatc cacagacgct ggcggcgtgg acgttgtagc
    12121 cgggtctgta gttgtcgacg tatttaacgg aacactctgc ccctgctcgc tattactgct
    12181 cagttccgcc gaagattgat cggccatagt ggtgatctct tcctgctgag ctttgtggtc
    12241 ttgccaccac caggcaccgc tcaggccgat aaccacaaac aacaccagcc aggtgaaggt
    12301 catcagccag ccgtcacgtt ttttgcggcg tttaccgagg gaaaaactct gcatcggcgc
    12361 aactttcgca gcccgtagtg gagcctgctt ttccagccct agcagcagtt cttcttctgg
    12421 aatatgtacc agacgcgcat aagagcggat atatccgcgc aggaatgttg aagcaagatc
    12481 ggcgggtgcc ttatcttctt caatgtcgcg taccgtggaa accttcaggc aaagtcgctc
    12541 ggcaacggcc tgctgactaa gtcctagttg ttcgcgagca ttacgcaggc gagcgccggt
    12601 agtaagtgct tcattttggt cgtgcgtggc ttcagtattc attcgctgca ggtacgttta
    12661 aatgagaatt gagatgccgg tgaaccatca tgctcaccca caccgcgaaa catccgttaa
    12721 gttaaccgtt atcatacagt ataagaccgt ctgtgtgctc gtgacaaagc ccacattcac
    12781 taaggctaac ttactgttgc atcgttacat actgccttaa agtcagcaaa aacgcaccgt
    12841 taatattgac cagacaattg caacttaata cttcattcat aaaagattac gccacggtac
    12901 ataaagtaac cgtggcgtaa tggctatcag accgctttaa tgtcgatggc ttcaccctgc
    12961 atccgtttac gcagggtacg tttcgtacgg tcgataacat cgcccgccaa ctgaccacag
    13021 gcagcatcga tatcatcacc acgagtttta cgcacaatag tggtgaaacc gtagctcatc
    13081 agcacttttg agaaacggtc gatacggctg ttcgaactgc gtccatacgg cgcgcccggg
    13141 aacgggttcc acgggatcag gttgatctta cacggcgtat ctttcagcag ctccgccagt
    13201 tggtgcgcgt gttcagtgcc gtcgttaacg tggtcaagca tcacgtattc aatagtgact
    13261 cggccctgat tggcgttgga tttctccaga taacggcgca ccgcagcaag gaacgtttcg
    13321 atattgtact ttttgttgat cggcacaatt tcgtcacgaa tttcgtcgtt cggcgcgtgc
    13381 agggaaattg ccagtgcaac gtcgatcata tcgcccagtt tatccagcgc cggaactaca
    13441 ccggaagtag aaagcgtgac gcgacgttta gacaggccaa aaccgaaatc atccagcatg
    13501 atttccatcg ccggaacgac gttgttcagg ttgagcagcg gctcgcccat gcccatcatc
    13561 actacgttag tgattgggcg ctgaccggtg acttttgctg cgccgacgat tttcgccgca
    13621 cgccacacct ggccgataat ttccgacacc cgcaggttgc gattaaagcc ctgctgggcg
    13681 gtggaacaga atttacactc cagcgcacac cccacctgcg aagagacgca gagcgtggca
    13741 cggtcgtctt ccgggatata caccgtttcg acgcgctgat cgccaacggc gatcgcccat
    13801 ttaatggtgc cgtcggatga acgctgttct tcaaccactt ccggtgcgcg gatttccgcc
    13861 acctctttca gtttgccgcg caacactttg ttgatgtcgg tcatctcatc aaagttgtcg
    13921 cagcaatagt gatacatcca cttcatcacc tgatcggcgc ggaagggttt ttcacctaaa
    13981 tctttaaaaa actcccgcat ctgctgacgg ttgagatcca gcaggttgat ttttccatct
    14041 ttcgtggtga cgttttcagg tgtgactaat tgttcagaca tatgctattc cggcctcgtt
    14101 attacacgtt atggcccctg gagggttgaa aaaagaaacg ccccggtgag cggctgctcg
    14161 tccgggggcg ctgcattgta caaattctgg cgcacggatg ccacgtttgc acgcggcatt
    14221 tacgaaatta ttaacgggtg cgcgggcaca cttcgccttc gccaaagaaa taagcgattt
    14281 cgcgagcggc agattcgacg gaatcagaac cgtgggtacc gttttcggtc aggctgtcag
    14341 cgtaatcagc gcgcagagta ccagccagtg cgtttgccgg attggtcgcg cccagcagat
    14401 cgcggtgacg ctgaacggcg ttttcacctt ccagcacgga aaccacgatc gggccagagg
    14461 tcatgaattc aaccagacca tcaaagaacg gttttccatc gtgttcagca taaaagccac
    14521 gtgcctgttc aacggtcagg tgcagcattt tggtgccgac aattttgaac cctgcagctt
    14581 caaagcgcgc aaagatatta ccaatgacgt tttttgctac cgcgttcggt ttgatgatgg
    14641 aaaaagtacg ttcaatagcc atttttacct ctgtaaattg ttctgttgtt gtctgtacca
    14701 gcgtacgaga tggcgcggat tataatgagc aacagggccg ttgactattg atgaaggtaa
    14761 cattttttta aaataaaatg agttttagca acaatcaggg acaaaacaga ctattgcatg
    14821 acaaatttca ctgtcgcgat ttgccccacg tcatccatca ccagcaattg ataatcacct
    14881 ttatccgtca aatgcagggt cacgttgcgc ccgcgttcag ttaacggttc gccattcaga
    14941 aaccaccagc gttcacctgc ccctccactt gattgcaacg gcaaagtcgc ttccgctgcg
    15001 cccggtaaac gtttaataat cgcgccatcg cgaacgcctg tcagctgcag cgggagctgg
    15061 gcatagtgac cgtatggcgg gcaacttgtc gaggccggtg gtaagcgcac agcgcgacgt
    15121 tctgatgcgg gcagccaggg ttccagcggt agcggccaga catttatcat ttcttgtcgc
    15181 gcttgcgggc aatcagcggc aacacgtttg ccattttcat ccagccagat ggggaaacga
    15241 atgccattaa tgccttcctg ctccggcagt aatagagttg gcggctgact cccgtccagc
    15301 agccaggttg ccaggcggcg gcgacagtta ccgtcacctt ccggcaaaga ctgtccgccc
    15361 ggccagcaga taacgccacg agtgactgag ttcgggcgcg ggtcttccgg cagattcgca
    15421 ctgcgcgaca gtaagatatt attgacctga ttcaacaatg gtacggcact ggcaaagcca
    15481 aactgaccaa caacgggcgt gcagtccggt ctgccagtcc agatcccaat gacatagcga
    15541 gcgttaaccc caatcgccca ggcgtcacga taaccatagc tggtgcccgt tttccatgcc
    15601 agtggggcga cgcgcggcaa ggcactatcc ggcaagggtt gcgcttcatc agccataatc
    15661 cggcgaatga tccacgccgc ccccgacgac attaaaggcc attcaagcag cggatcgtca
    15721 ggctgtaagc gcaatttgcc tgccttgccg tggcgagcaa acgcggtata cgctgccgcc
    15781 atatcttcca gttttgcacc agcgccgccg agaatgagtg aaagattcgg cgcagcaccg
    15841 ttgggcaaat ataacggcaa tccaacattg cgtaactttg ccgcaaaccg tttcggtcca
    15901 taggcttcca gcacctgcac agcaggtaag ttcagcgagc gcaccagcgc ctcgctcatg
    15961 ctgatcgggc catgaaaacc gctatcaaag ttacctggtc gataatcacc ggtgcgccgg
    16021 gggacgtctt gcagcagtga tgccgggtgg atcaagcctt catccagcgc cagaccataa
    16081 acaaacggtt tgagcaccga tcctggcgat cggatcgcat tgaccatatc aacatgacca
    16141 aagcgtgaat catcgttgag atcaaccgat cccacccagc cgcgaacacg catatcggta
    16201 tgatcaacca cgatcatcgc cagtgagctg cgcggtggca accgcccttt ccagttttgc
    16261 gccagttctt ccagacgtcg ttgaagaccg gcatccagcg tagtaacgat tttgtcgctt
    16321 ttgcttttac cgagcatcat gcgcgaaaac agcggtgcca gttgcggcat ttgtcggggt
    16381 gccagccaga tgggttcttc ccttgactct tttacctgct cacgggacca cacaccttgc
    16441 acggccatcc gttcgagcac tttattacgc gcggcttcgg cacgctccgg ccaacgatcc
    16501 gggcgcagtc ggctgggcgc ttgcggtaac accgccagca ttgccgcctc ggaatagctt
    16561 aaattcgcag gcgattttcc gagataagcc caacttgccg caccgatccc ctgcaacgta
    16621 ccgccaaacg gagcgcggtt aagatacaag atcagaattt cacgcttaga caaatgccac
    16681 tccagttgca acgcgcgcca gagctggaga attttgccac caaatgtttt ggggtgagga
    16741 tcaagcagac gagcaacctg catagtgagc gtgctgccac cagaaataac ccgtcccgaa
    16801 gtgagatctt gccatgctgc gcgcgccacc gagaatggat tcacccccgg atgcttccag
    16861 aaccagcgat cttcataatt gatcagcgct tcaaggtaac gtggagacac atcttcgatt
    16921 gttaccggat aacgccagat gccatcagca tcggcgaagc gccagagcgg cgtaccatcc
    16981 tgcgccacga ccactcgtgc gggattgact tcatgcagcg gtagaggcca gagtttatct
    17041 gctccccacg ctgcaagaaa aaggagaaat ggcacagctg ccagcgttat ccagcagccg
    17101 cgtttggtta acaagcgaag catttacggt cggacaatca gcagatcttc agccgcgccg
    17161 gtcgcccgcc attggggaac atacattgat tccaccatcg gttgcggtac ctgatacgtt
    17221 cccggcgtca ccgcccgcgc cagatacacc agcgttaccg gttggtattc atcaacggca
    17281 accgccgcca caaagcgatc gtcacggaac tcaatgtgct taatgctcgc ctgctgcatc
    17341 tggttcagta agttttgcac ttcgtcacca ctttgctcca ggctggcgct accgttcgcc
    17401 agattctgat tttccagttc cagacccgca ggcagcagat ccacgactaa cgcatccggc
    17461 acgctgttac tggcttttac ctgcaaccag accagcacca gatcgccgct acgtaacgag
    17521 tccagcgatt tgctcttacc atcagtacca agaatatgac gctcgatttg cagcacattg
    17581 ttcgccggta aaggtgcgga ttgcggataa ccgctggcat ccatacgcaa ccataacggc
    17641 tgatcgccac tgtttctcac ctgcaaggtg gcaagttgat cgctattcag attgctgttt
    17701 agcgttttct cgcctgtcag cggctcagct gagaaagagg tttgcgcctg ccatttaccc
    17761 ggtaaatcct gaatcgtagg ggcagccagg aacaacgcgt tactttcctg cgtcgatagc
    17821 cagcgttcac caaacgcctg ctgcgaaagt gtgttcagca aagtgtactg ctcatcgggt
    17881 agcagtttat tttcttccag caaggagagc attaacgcgt tgtcgcgcag tggactaccg
    17941 taatcaccca gccatatccg ctcatcacta tttctcggcg ttttcagcgc cagcacaatc
    18001 gcttcttcac cacgcgttgc atcacccatg gttttcagcg caatgccaag ttgcagcagc
    18061 ggtaaaccag aagcggcatc tgcacgatgc tcccagattt cacgcagcgc acccagtggt
    18121 gctttttgct gacgggccag caccagcgca gcgtaagact gaacggcgaa tttactggct
    18181 ttgagattat ccgcgtacgg gatcgacatc atgcccggat cttgtaaata gcgcagcaga
    18241 cgctcattac cccggttaat ggcgtctgtc ggcacgctgt aaccctgttc gcctgcgcgg
    18301 accaggaaat ccatcacgta agccgtcagc cagtactctt cgtcaccgtt tttatcccac
    18361 agcgcaaagc cgccgttatc acgttgcatt tgcagcaaac gggaaatgcc gatatcgacc
    18421 gatgcacggc gtttctcatc actgtcgcct ttgatgccca acgcctgcag ttgggctgcg
    18481 ttggtataaa gtgacgggaa caggccgctg gcggtttgct caagacagcc gtacggatac
    18541 gcttttaact ctttgatata acgtgcaatg ttcagcggtg gtttgccgct caacaacaat
    18601 tgcccttcca gcgtaacagg cgagaagttt tgcaatccat ccgccggaat cgcccatgtc
    18661 tcaccaggct gtaacgccgt accgtaatta accgtttgtg ccgggaacgc cggacggacg
    18721 ccgattttcc actgcttatg ctgatcggca acggtttcac ccggtaacgc taacccgcta
    18781 atggtggcct gaatttctcc atcgccataa cccggcaatg ctcgcaccgg gataaacagc
    18841 gtagtacgca cacctggcgc taattcaacg gctgcgggtg aatcgctgac cagttcaagc
    18901 aaaccactgg cggtcagggc aacgttcagt ttttgcggtt tatcggtaag attagtgata
    18961 tccagcgtca gacgcgaggt atcgccactc gccataaagc gcggcatgtt cagttcagca
    19021 atcaccggtg cggcaactat cactttgctt tcgttgctac cgaaatcatc tgccgtccag
    19081 gcttgcgcca tgacgcgcaa ttcaccgtta aaatcgccaa tcggcagtgt aaccgagcct
    19141 tcgccctgtt cgttgagcgt taccggcagc gcctgctgcg cgacaatatt gacgtgattg
    19201 accagcggtt taccaccacg tttcagctca tcaccatcgc caccgaaacg cagagctgcc
    19261 agacgcccct gaccttcaat aacctgaccg taaatatcgt aaatgtctgc gccatagcgt
    19321 ttctgaccaa agaacgcctg ccacggatct ggcgtgacgt agtcagtaat attcagcaca
    19381 ccactatcaa cggcagacac cagcacattc acctgtttag gcttctcgcc atttttagtg
    19441 ctggctttaa ttttcacggt taatggttga ttgggacgca tttttgctgg tgtttccagc
    19501 gccagatcga ggcgacggtt ttcatcgcca agcggcagat gcaacacacc aaccgcgcgt
    19561 tttggcgtcg cggagcgaga tttatcgcta ggacgtacca ccagcgtact taaatacaga
    19621 tcatgacgat tccaggtttt atcgaccgga atcgtcagat ccagcccttg agccggaaca
    19681 tcaatctctt gccaccacag cggcccttca ctggactcga ccatcgcata acctttaccc
    19741 gccgttggcg cggcgatatg caacttaatg gtgtcgccag ggcgataact ggctttatcc
    19801 agtttcagcg tgacacggtc gggtcgcact gcgccgctac cgtcgctgtt gtcctgccag
    19861 ctatagccag cccagaaacg aacactactg accgcttcat tcggcgcttt gacttccaga
    19921 cgataagcgc cccactctac cggaaaactg accttgccgg tttcgtccgc tttcagatcc
    19981 agagtttgtt cattttcgat cagatctttt tgatcaaact gtgactgcca gccttcatct
    20041 tctgaccagt tccagtagta atcgcggcgt tcgcgaatca ggcgcacctg caagcccgac
    20101 acggcttttt tcacgccttg tgcatcgcta taaacgatgt caaaagcggc gttactgcct
    20161 tcatcaacaa tgggctgttt caccgtgctg tcagtgcgat aatcgtaaac cgatttcaag
    20221 gcgaactgcg gacggagccc cggcaatgca ttggcaggcc agatagcctg ctcagcgcgg
    20281 cgcgtcaccg ggcgaccgcc cgattccagc aaactgccct ggaaaataac ctgcaatggg
    20341 gaatgcgttt ccttccactg gctttctgta gaaacttcgc cgcgcccttt atcatccagc
    20401 gtcaactgaa cttcatccag cgtgcgggaa aggttttcgg cagctatatc gccgaattca
    20461 aaaccaggta aggctgacac agcctcacgc agtgggcgca ggaaaagttg cccttgcaaa
    20521 gtattaccat tagcaggcgc accgtacagg tagtatccca ccacggagaa tttcacttca
    20581 tcattcggcg taagcggggt tttctcaccg gtcagattca gcgccatacg ctctggcata
    20641 aaatcttcga cgtggaaatc ccacatccgg tactggttat cgcccgtgtt ggcgcgaata
    20701 tgccacatac cggttgccgc attgctatcg agtggccagg taaagtggta gaggccattc
    20761 tccggctgac taacgacgct cctgagtacc tgcccgtcag gtttaatcac gtctaacttg
    20821 atgggttgat cgggcaatgc tttaccgtct gcatcgcgca gtaaaccatt gaggattacc
    20881 gtttcacctg ggcgataaag atcgcgtggg ccaaacatga aaaactgttt gctatagcct
    20941 ggcgcgccag caatgttaaa ttctgctaag tccagcgccg gaagttttaa atcgagtagc
    21001 gttgtctgac cgtctttacg tgccagcagt aatgccgcat ttttatcatt ttccagctgc
    21061 acatgcccct gagcgtcact ggttgcctga gtcagagtct gccctttctc atttaataaa
    21121 gagacttcaa ttccttgctg ggccgcgccg ttttccagac tttgggtaaa gatatccaga
    21181 cgattgtgat aacggtgagc tgaaacgccg atatcactta acgtaaacag cgtcgcggga
    21241 ttactgtaat cgtaacgtcc agcctgattc atcacagcca gatacacgcc cgcctgctga
    21301 agcggtttga tatcgcccag cggcagcaat aatttttcac gggtgttacg cgcaggattg
    21361 agatcaaacc gtccggtgta gaccagatcc gccatctgca gcagtttgtc tgactgccag
    21421 ttcgccagcg aattgcggta ttcccattgg ctaatgaatg ctggcagaga ttctggctta
    21481 acgcggaaga agttaacatc gacattatta acgttgagcg ccattaccgg cagcccttca
    21541 acgactttgc caggcagcag cgaaccacgg ctggcaaaac cgacgctggg ttggatgtcg
    21601 cgggtagtta tagttttttc gtaatcttta ctgaaggttg cgttgttgag tgctttgacc
    21661 tccttgccaa tagtaacgat caaatcacgt ttcggttcga ggtggcgtaa acgtagctct
    21721 ttaagattat ctgacagctc ccaggcacca tccactttgc cgcttttttt atcgacgaca
    21781 tgaataacgc gtgagaaatc ctgatccggg tcgagaggga tggagaacgt cagcaccagc
    21841 gtggctgcac catcaagttg gacttcagag agatccagca atgtcagcac cttaccggca
    21901 ctctgttggg ccagtttttg tctctccggc acggagagtt ttgctgaact cgcgttttct
    21961 gaagaggccg ctttagtaac ttcagaaggc gcatcttttt tcaccgctgt tggcgcgtta
    22021 tcgttgttgt cgcaccctgc cagcgccagc attagcatgc aggcggctac gcgtaacttt
    22081 ttcatccctg ccacaatggc ccgttagcaa cgtcgaataa ttattatgcg tgagaattta
    22141 cgttatgtaa ttcagtatca cgcccataca atccgaattt tagtgagaat tgtggctcag
    22201 acagccaaat cagcctcttt cgcttgtcgc ttttcactaa acatccgaca atttagccta
    22261 accccggcaa aaatggagat gcctatgtcc acgacatggt ttgtaggagc cgactggctc
    22321 gccgaacata ttgatgaccc ggaaattcag attatcgatg cccgcatggc gtcgcctgga
    22381 caggaagatc gtaacgttgc tcaggagtat ctgaatggac atattcccgg cgcagtgttt
    22441 tttgatatcg aagcgctttc tgatcacact tccccgcttc cgcacatgct gccgcgcccg
    22501 aaaacgttcg ccgtggcgat gcgtgaatta ggcgttaacc aggataagca cctgattgtc
    22561 tatgacgaag gtaatctttt ctcagcccca cgagcatggt ggatgctgcg cacctttggt
    22621 gtagagaaag tgtcgattct ggggggtgga cttgcaggct ggcagcgcga tgatctgctg
    22681 ttagaagaag gtgcagtaga gctgccggaa ggagagttta acgccgcgtt taatcctgaa
    22741 gccgtggtga aagtaaccga tgtattattg gcaagccatg aaaatacggc gcaaattatt
    22801 gatgcccgcc cggctgcacg ttttaacgca gaagttgatg aacctcgccc aggtttacgt
    22861 cgcggacata ttcccggagc actgaatgtt ccgtggacgg aactggtgcg cgaaggcgaa
    22921 ctaaaaacga ccgatgaact ggatgcgata ttttttggtc gcggcgtcag ctacgacaaa
    22981 ccaattatcg tcagctgcgg ctctggtgta acggcagccg tggttttgtt agcactcgcg
    23041 acgctggatg tgccaaacgt gaaactgtac gacggcgcat ggagtgaatg gggcgcgcgg
    23101 gcagatttac cggttgagcc agtgaaataa gtattttaca ggcaataaaa aaccgccgaa
    23161 tttggcggtt ttttattgct ggtctggttc gcggcctttc cagcaggttg tattaccgta
    23221 gtaatgcaag cgcgtctcag cggagacaat actcgccagt aactctcttt ttgtcaagca
    23281 aaagagagtt attattgttc tgttagtata ttatccactg cggccctttc cgccgtctcg
    23341 caagcgggcg gtggcttaag gaaaggatgt tccgcgaccg ttagagcaag cgcgtctcag
    23401 cgcttgctat cgcggcaata tcgccagtgg tgctgtcgtg atgcggtctt cgcatggacc
    23461 gcacaatgaa gatacggtgc ttctgtatcg tacttattgt ttctggtgcg ctgttttccc
    23521 aagtaaataa taaccggagt ctctccggcg acaatttact ggtggttaac aaccttcaga
    23581 gcagcaagta agcccgaatg ccgcccttta gggcggcata ttttagatta tccggttttg
    23641 tttaaagtca cgcaaaaaac ctccccagcg acgttcatag aatggcacaa tatgttcggt
    23701 aataaagtgg ctaattcctt tttccccttt tttcacctga caaatatcga ttggttcatc
    23761 gccaggtaat gtatcggtcg ctacacttcc cgtcgcctga ataatttctt cgatatcacc
    23821 atcggcttca atgccaataa ataaattagg ctgtgcctct tcgttctctt taattgaaca
    23881 aataaaagca cgcttcaccg gcttaatggt tttaaataag gtggtgagtg aatcaatcat
    23941 ttgtgctggc ggctctgcaa cttccgataa tatcagcgat tcaccgcctt ccaggatttc
    24001 ctggctgctc agcggatttc cctcttcacc aatcaacaaa ctgatttcac gcggcataaa
    24061 ttctttaccg gtcggcagtt tggcattaag gaagagcgtt tcgccaaggg tcatctcaaa
    24121 caatgtgcga acgggcatta cgacaaatgc ctgttcgtct tcaaccgcct gttgaagcgc
    24181 ttctaacgag gtgaaaaaag gaatgacgct ggtgccgtct tctttttccc agtgctgtaa
    24241 atcaagcgca ctatcttcaa ccacagcctc gccctgcgcc gccgtaccag gcacccagac
    24301 ggtggattcc agtagagtac ggaaaaaggc cgggcggtgt gccggttcgg ttgctgcttt
    24361 ttccagcagg tcttcaagtt cgttttttgt ttcggacata agaaccacaa ttcattcaac
    24421 gttcggggcg caaatgcctg atgcgctacg cttatcaggc ctacaaggga ttcgcaattt
    24481 gttgaatttg cagaatttgt aggccggata aggcgtttac gccgcatccg gcatattagt
    24541 ttacgccgtt aacagattag ctatcgtgcg cacaccaagt cccgtagcgc ccgcagacca
    24601 ctgttcaacc ggcgctttac ggtaagtcgc cgagcagtcg atatgcagcc agccttgctg
    24661 atagttctca acaaagtgcg acaggaagcc cgccgccgtg ctcgcgcctg ccggatacgc
    24721 cgcgcttccg gtattgttca gttcggcaaa gttagacggc agctggctgc ggtggaattc
    24781 cgccagcggc agacgccaga atggttcgtt ctcttgtgag gcactcgcca gcaaacgacc
    24841 ggcaagcgca tcgtcaaaac tgaacagcgc gtgataatca ttacccagcg cagttttcgc
    24901 cgccccggtg agggtcgccg catcaatgat catttccggt ttctgcgcac tggcatcaat
    24961 cagaccatcg gcaagcacca gacgcccttc ggcatcagtg ttcatcactt caactttttt
    25021 accgttgcga taggtgatga tatcgcccag cttgaacgca ttgccgctaa tcaggttatc
    25081 cgcacagcag aggaacagct tcacgcgctt gttcagtccg cgcgtaatgg caaatgccag
    25141 cgccccggta accgttgccg cgccgcccat gtccgacttc atcgagtcca taaacgcagt
    25201 ctgtttgatg ctgtagccgc cggagtcaaa agtgatacct ttacctacca ggcacgcgta
    25261 cactggcgct tctttatcgc cagttgggtt gtaatccagc gccagcaata ccggagaacg
    25321 ttctgaagcg cgtccgacgg tgtgcagccc catataacct tgctcacgca gatcttcgcc
    25381 tttggtgatc cgataagtca cacgatcgcc cgcgacgttg ctgatcagat caacagcacg
    25441 ctgtgccagt tgcgatggtc ccaattcttc tgccggtgcg ttgatggtgt cacgcaccca
    25501 gtcgatgatc atcaggcggt tatccagttc ctggcgctgg gcatcgtcca gatccggcca
    25561 ctccacttta cgcgtgcctt tcggggcttt gtaaccttgc cagaatgccc agcagcgatc
    25621 cgcatcccag ccttcaccgc ttaactgaac atgcttgatg cccagaccgt caatcttgcg
    25681 cgccgcacgc tggatcagcc ccagatcgtc tgccccgttc aaatgcaggg taatgccgtc
    25741 attattaatg ctgtaagttg ctttttctcc ccagcgcgcg tcggcaggtt gggtagagag
    25801 ggtaatcttc atcgcttctg tcattttagt tatccttctt tgtaaaaggg ccgccagttg
    25861 gcagccccag gatttttccg ctaaatgatt cgcgttgcag gaaggcggca agtgagtgaa
    25921 gccccaggag catagataac tatgtgactg gggtgaacga gcgcagccaa cgcatctgcg
    25981 gcgtgaagca tgacgcggaa attattcggc ctcgtccagc cagactaaca aaatcgcttc
    26041 gaggattttc tcgttggatg cctgcgggtc gtcgtcgaaa tcttccagat cgcaaatcca
    26101 ctgatgcata tcggtgaatc gaaccgtttt cggatccaga tcaggatacg catcgtacag
    26161 tgcttcgcca atttcgcggc tatcggtcca cttaagtccc atactaacct ctgttaatgc
    26221 tcacgcgcat ggttgatagt gtaacgcggg atttcgacta ctaaatcttc gtcggtaacg
    26281 cgtgcctggc agcttaaacg gctttcgggc tccagtcccc aggctttgtc cagcatgtcg
    26341 tcttcctgct ctgagctttc tggcagcgag tcaaaacctt cacgaacgat gcagtggcag
    26401 gtggtgcaag cacaggattt ttcacaggcg tgttcaatct cgataccgtt acgcagcgct
    26461 gcgtcgagaa tggtttcacc gctattagct tccagaacag cgccatcagg gcagaggtcc
    26521 tgatgaggca aaataacaat ctttggcata ttaaacctcg tccacggaat ggcctttcag
    26581 cgcacgacga accgactggt ccatgcggag agcggcgaaa tcctgggttt gtttgtctac
    26641 gtttttaatc gcttgttcga tggcgtcaac atcatcgccc tgagccactt cactcaggtg
    26701 agcggcagca ttgtcaatga cctgacgctc tgcggcgctt aacagcgcgg catcagcagc
    26761 cagcgcgccg tgcagacttt ccagcacacg cgccgcttct actttttgtt ctgccagcat
    26821 acgggctttt acgtcctgct cggcatagct cattgaatct ttgatcatcg aagcgatttc
    26881 gctatcggtc agaccgtaag aaggtttgac ctgaatagac gcctcaacgc cggtggattt
    26941 ctccatcgcc gtcacgctca aaagaccgtc ggcatcgacc tggaacgtca cgcgaatatg
    27001 cgcaccgcca gccggtagcg ccggaatacc acgcagcgca aaacgcgcca gtgagcggca
    27061 gtcctgcacc agttcgcgct caccctgcat tacatggata gacatcgccg tctgaccatc
    27121 tttaaaggtg gtgaaatcct gagcgcgggc caccggaata gtggtattac gcggaatcac
    27181 tttctccacc aggccgccca tcgtttcgag gcccagcgac agtgggatca catcaagcag
    27241 cagcatttcg ctgtccggct tgttacccac cagaatatcc gcctgaatcg ccgcgccaat
    27301 agcgacgact ttatccgggt cgatggaagt cagcggtgga cgaccgaaaa attcgcctac
    27361 ccgttcacgc accagcggca cgcgagtaga accgccaacc atcaccactt ccagcacttc
    27421 atcagcttct acgcccgcgt ctttcagcgc acgacgacaa gccagtaagg ttcgttttac
    27481 cagtggcgcg atcagttcat tgaattgttc acggctgatt tcgccctgcc agcccgcaac
    27541 gttaacggtc acggagtccg catcgctcag cgcgattttg gctgcaatgg cggcatccag
    27601 cagttcacgc tgaacgcggt tatcgctacg atcaggaatg tccgcctgct cgcgaatgta
    27661 atccgccagc agatggtcga aatcatcgcc gccgagcgcg gaatcaccgc cggttgccag
    27721 cacttcaaac acgccgcgac ttaagcgcag aatggaaata tcaaacgtcc cgccaccgag
    27781 gtcataaacg gcgatcacgc cttcctgacc ggaatccagc ccgtaggcga tagccgcagc
    27841 ggtcggttcg ttaagtaagc gcagaacatg caaccccgcc agacgcgccg cgtctttggt
    27901 gccctgacgc tgggcatcgt caaagtacgc cggaacggtg ataacgacac catccagctc
    27961 gcctgccagg gcttcagttg cccgcgccgc cagtgctttg aggatgtccg cagaaacgcg
    28021 caccgggttc agcagccccg ccgccgtttc aatcattggc aggccgtttt cgctggcctg
    28081 gaattgataa ggcaaatgcg gatagcgttg ctggatatca gccagcgagc gtcccatcag
    28141 gcgtttaaca gaactaatgg tgttggcggt atcgagcgct gcgttagtac gcgcgtcata
    28201 acccaccgaa tgcccttgct gttgatagtg aacaacagat ggcagcaggt gacggccttc
    28261 atggtcggct aacgtttcgg cctgaccgct gcgcactgtc gctaccagcg agttggttgt
    28321 gcccaggtca ataccggccg ccagacgacg ctgatgcggc gcagcactca aaccaggttc
    28381 actaatttgt aataaggcca tgtttagctt ccagaaatta aaaatcgagc agtttttctt
    28441 cgagttgttc ggcactgctt cgcagtttat cgagaaaacg cagcttacgc acggtatccg
    28501 ccgccgcgtc ccacgtctcg ttgtctaact gttcaaccat caactgatgg cgggtatcaa
    28561 acatcttttt cacacgtttg ataaagcttt ccagccgcgc ttcatctttc gcctgttcga
    28621 tctcgtccag ttcttcgcgc agctccagct gttccatcag gaacgcggtg tcgcgcacag
    28681 tatgctgctc gctggcgaga tcaaaaccgt gcaaagaaag caaatattcc gcgcgcatta
    28741 acggatgacg cagcgtttgc caggcctggt taatggttgc agattgctgt acggcggcga
    28801 gttgttccgc ctggcttccg ctggcgaatt tatcaggatg atactgacgt tgtagatcct
    28861 gaaaacgcag gctcagtgcc tgggtatcga gttgataacg ggcaggcaag ccaaagaggg
    28921 tgaagtaatc cataacattc tcagggctgc gatccgccgc gataacggcg aatcaggtaa
    28981 aacaaacccc acgcgcaggc gaccacggtg gggttatcgg tatgcgcatc aaacgtggaa
    29041 gctttcgccg caaccacact catctttaac gttcgggttg gtgaatttaa acccttcatt
    29101 caggccttct tttacgaagt ccagctgcgt accgtccaga aattgcaggc ttttgccatc
    29161 gaccacgact ttcacgcctt tgtcttcaaa cacgatgtct tccggcgtcg gttcgtcaac
    29221 aaattccagt acataagcca tacctgaaca tccggaggtt ctcacgccca gacgcaggcc
    29281 aaaccctttg ccgcggttag ccagaaaggt atttactcgc gctgctgcac tgtcgctcag
    29341 tgtaatcgac ataaccaaac ctcaactctt attttgcttc acgtttgctt ttatagtccg
    29401 caatggcggc tttgatcgcg tcttctgcca gaatagaaca gtgaattttc accggcggca
    29461 gttcaagttc ttcagcaata tcggtgtttt tgatcgcctg cgcttcgacg agagacttcc
    29521 ctttcaccca ttcggtgacc agggagctgg aagcgatagc agaaccgcag ccgtaagttt
    29581 taaaacgcgc gtcttcaatg ataccttcat cgttgacttt aatctgcaac ttcatcacgt
    29641 cgccacaggc cggtgcaccc accatgccgc tgccgacgtt ctcgtcgttg ttgtcaaagg
    29701 aacccacgtt acgcggattc tcgtaatggt cgataacttt ttcgctgtaa gccattataa
    29761 attctcctga ttccgatacc gattaatgat gagcccattc gatgctgttc agatccacgc
    29821 cctgcttgta catttcccac agcggagaaa ggtcacgcag acgaccgatg gatttacgaa
    29881 ctaactcgat ggtgtagtcg atctcttctt cagtagtaaa acgacctaaa gagaaacgga
    29941 tagagctatg tgccagctcg tcgttcagcc ccagcgcgcg cagcacgtag gacggttcga
    30001 ggcttgctga cgtacaggcg gaacctgaag aaactgcgag gtctttcagc gccataatca
    30061 gcgactcacc ttcaacgtag ttgaagctga cgttgagaat gttcggcgca ccgtgttcca
    30121 ggtcaccgtt caggtaaact tcttcgatat ctttgatgcc gttccacaga cggttacgca
    30181 ggccgcgcag acgttccatc tcggtcgcca tctcttcttt tgcgatgcga taggcctcgc
    30241 ccattccgac gatctggtga acaggcagag tgccggaacg cataccgcgc tcgtgaccgc
    30301 cgccgtgcat ttgcgcttcg atgcgtacgc gcggtttacg acgtacatac agcgcaccga
    30361 tacctttcgg gccatagatt ttgtgaccgg agaaagacat caggtcaact ttcaactggc
    30421 tcaggtcgat aggcagtttg cccacgctct gggttgcatc aacgtgatag ataatgccac
    30481 gagcacggca catttcgccg atagccgcga tatcctgcac cacgccgatt tcgttattta
    30541 cgtgcatgat ggaaacaagg atggtgtcgt cacgcatcgc tgcttcaagt tctttcaggt
    30601 cgataatgcc gttacgctgc ggtgccaggt aggtgacttc aaaaccttcg cgctccagct
    30661 gacggcaggt atccagtacc gctttgtgtt cggttttgct agtgatgatg tgcttgcctt
    30721 ttttctgata aaagttggct gcacctttga tcgccaggtt gtcagattcg gttgcaccag
    30781 aggtaaagac gatttcacgc ggatcagcgc cgaccagatc ggctatctga ttacgggcga
    30841 tatctaccgc ttcttcagcc tgccagccga aacggtgaga acgagaggcc gggttaccaa
    30901 aggttccgtc catcgtcatg aactgcatca ttttctcggc aacacgcggg tccaccggcg
    30961 tggttgcgga gtagtcgaga taaatcggta atttcattgc tctataaact ccgtacatca
    31021 ctcaatgcaa ggaatcaggc taccggctgg atgtacgacc gtgtttacgg agtatttagc
    31081 actccggcct gattctgaat tctttttatt aagcgcgtaa cttaacgtcg atcgcgtctt
    31141 gtgtgcgggt gcgtggcgcg tcgtgagtat gctgacgacc agacacatcc agcacttcct
    31201 ggttattaac cagttcgcct aaagtaatgt tgttgagaaa accggtgaga cggtcgctca
    31261 aatcacgcca cagcgcgtgg gtcaggcatt tatcgccgcc ctggcagccg cctttaccct
    31321 gacaacgggt ggcatctaca gattcgtcaa cggcgctaat cacttcgcca acggcgatgc
    31381 tgctggcatc tttgcctaac agataaccac cgcctggtcc acgtacgctg gaaaccagac
    31441 catttttacg cagacgggaa aacagttgtt ccagataaga aagggaaatg ccctgacgtt
    31501 cggaaatatc agccaacggt accgggcccg cttcagagtt gagcgcaacg tcaagcattg
    31561 cggtcacggc atagcgccct ttagatgtca gtctcatgtc ttacttcacc tcaaactcgc
    31621 ccctgcccgg ggttttttat tgtaaaagtg ggggtattga atagcagggt caagtctgac
    31681 attcccgagt aaattggtca actatttact tgactgattt agtcgggtat ttaaccttca
    31741 gtgccatttt tttatcgtgg cgtttgtacc tgttgtcgga tgcggtgtga acaccttatc
    31801 cgacctacgg ttctgtacat tgtaggcctg ataagacgcg ttaagcgtcg catcaggcaa
    31861 ctactgttat tcggccttgt tacctttatt ctgctgctca atagaagcca gaatcccgcg
    31921 caggatattc aattcctggc tttccgggcg cgcacgggta aacagacggc gcaatttatt
    31981 catcacctgc cccggatggt tttcacggat aaaaccggtt gccagcagcg tttgttccag
    32041 atgaccataa aaacgctcca gatcatcgac cagcggatac ggcgtctctt catgctcgac
    32101 ctgctcgccg ttttcctgag ttgccagcca ggccatgcgc acttcatagg caataacctg
    32161 aaccgccatc gccaggttca gcgagctgta ttccgggtta gccgcaatcg cgacatgata
    32221 atggcatttc tgcaactctt cattggtcaa gccgacgcgc tcgcgaccaa acaccagcgc
    32281 caccggggta tttgccgctt cagcgacgct tttcaggccg cattcgcgcg ggtcgagcat
    32341 cggccatggc agcgtgcggg aacgtgcgct ggtgcccacc accaagctac aaccggctaa
    32401 cgcttcgtcg agcgtatcga cgatgtgagc attaccaatc acatcgctgg cccctgctgc
    32461 cagggcaatc gcctgggagt cgggtttcac cagtggatta accagccaca gattggttaa
    32521 tcccattgtt ttcatggcac gggcaacaga acccatattg ccggtgtgtg acgtctccac
    32581 cagcacaatt cgaatatttt gcagcattgt ctttcttcat ctaaagatta ttcacgcatc
    32641 ttatcataaa acgaagacag atgccgatct cgctgctata ctctgcgccg ttttcccgtt
    32701 ctttaacatc cagtgagaga gaccgatgca tccgatgctg aacatcgccg tgcgcgcagc
    32761 gcgcaaggcg ggtaatttaa ttgccaaaaa ctatgaaacc ccggacgctg tagaagcgag
    32821 ccagaaaggc agtaacgatt tcgtgaccaa cgtagataaa gctgccgaag cggtgattat
    32881 cgacacgatt cgtaaatctt acccacagca caccatcatc accgaagaaa gcggtgaact
    32941 tgaaggtact gatcaggatg ttcaatgggt tatcgatcca ctggatggta ctaccaactt
    33001 tatcaaacgt ctgccgcact tcgcggtatc tatcgccgtt cgtatcaaag gccgcaccga
    33061 agttgctgtg gtatacgatc ctatgcgtaa cgaactgttc accgccactc gcggtcaggg
    33121 cgcacagctg aacggctacc gtctgcgcgg tagcaccgct cgcgatctcg acggtaccat
    33181 tctggcgacc ggcttcccgt tcaaagcaaa acagtacgcc actacctaca tcaacatcgt
    33241 cggcaaactg ttcaacgaat gtgcagactt ccgtcgtacc ggttctgcgg cgctggatct
    33301 ggcttacgtc gctgcgggtc gtgttgacgg tttctttgaa atcggtctgc gtccgtggga
    33361 tttcgcggca ggcgagctgc tggttcgtga agcgggcggc atcgtcagcg acttcaccgg
    33421 tggtcataac tatatgctga ccggtaacat cgttgctggt aacccgcgtg ttgtgaaagc
    33481 catgctggcg aacatgcgtg atgagttaag cgacgctctg aagcgttaat gactcaggcg
    33541 ggtgatgtca ctcacccgcc ctcgcctttc aggcgctatt ccgaaatact tcctcaccgc
    33601 tttactttct ttcgtcactc tcccaccatc tttccccgat taatggatga taaagaagtc
    33661 acaaggaaag gttatggcgc tgccagtgaa caaacgcgtt ctcaaaattc tgtttattct
    33721 ctttgttgtt gccttctgcg tttatttagt gccgcgcgtt gccatcaact tcttctatta
    33781 tcctgacgat aaaatttacg gtcccgaccc ctggtcggcg gaatccgtcg aatttacggc
    33841 taaggacggt actcgtctgc aaggctggtt tattccttct tcgacgggcc ctgctgacaa
    33901 tgccatcgca accatcattc atgctcacgg caatgccgga aatatgtccg cccactggcc
    33961 gctggtcagt tggttacccg agcgtaattt caacgttttt atgtttgatt atcgcgggtt
    34021 tggtaaatca aaagacacgc cgtcccaggc cggattgctg gacgatacgc aaagtgccat
    34081 caatgtggtg cgccatcgca gtgatgtaaa cccacaacgg ctggtgctgt tcgggcagag
    34141 tattggcggg gcgaatattc ttgctgttat tggtcaggat gatcgtgaag gcatacgtgc
    34201 ggtgatcctc gactccacat ttgcctctta tgcaaccatc gccaatcaga tgatccccgg
    34261 cagtggctac ttacttgatg agagttacag cggcgaaaat tatatcgcca gcgtcagccc
    34321 gatcccgctt ttactcattc acggtaaagc tgatcacgtt atcccatggc agcacagcga
    34381 aaagttgtat agcctggcaa aagagacaaa acggttgatc ctaatcccgg atggcgaaca
    34441 cattgatgct ttttccgatc gtcacggcga tgtttatcgc gaacagatgg tggactttat
    34501 ccttagcgcg ttgaatccgc agaactaacc catgatcgct agcacgataa tcattcacaa
    34561 aaccacctta agacatgcta atccactggt cagaacagtt taagatgaga aaaattctgt
    34621 gacgcttgcc aacatttctg atgattagca ttcccttcgc catttacctg agcaaacttt
    34681 agctattctt atcaattatg cttatgggag atctacagat gatgcctacg cttgctccac
    34741 catctgtcct ttcggctccc cagcgccgct gccagatctt gctgacgctc tttcagccgg
    34801 ggttgaccgc caccatggca accttcagcg agcttaatgg tgtggatgat gatattgcca
    34861 gtcttgatat cagcgaaaca ggacgggaga tcctgcgcta tcatcaactc acactgacga
    34921 ctggttatga cggtagctac cgggttgaag gtacagtgct taaccaacgt ttgtgtttat
    34981 ttcactggct acgacgtggt ttccgtctgt gtccgtcatt tattaccagc caattcaccc
    35041 ccgccctgaa gagtgaactg aagcggcgcg gaattgcgcg taacttttac gacgatacca
    35101 atctacaagc gttagtgaat ctctgctccc gacggctgca aaaacgcttt gaaacgcgcg
    35161 atattcattt cctgtgtctg tatctgcaat attgtttgct gcaacaccac gctggaatta
    35221 cgcctcagtt taatccgctc caacgtcgct gggcggaatc ctgccttgaa tttcaggtag
    35281 cgcaggaaat tggacgccac tggcagcgtc gggcgctcca gcctgtaccg cctgatgagc
    35341 cactgtttat ggcattactt ttttccatgt tgcgggttcc cgatccattg cgtgatgcgc
    35401 atcagcgaga cagacaattg cgtcagtcta tcaaacgtct ggtaaaccat tttcgtgagc
    35461 tgggaaatgt tcgtttttat gatgaacagg ggttatgcga tcagctttat acccacctcg
    35521 cccaggcgtt aaatcgcagt ttgtttgcca tcggtattga taataccctg ccagaagagt
    35581 tcgccagact gtacccacgc ctggtgcgca ccacccgcgc ggcgctggcc ggatttgaaa
    35641 gtgaatacgg cgtccatctt tctgatgagg aaagtggtct ggtcgcggtg attttcggtg
    35701 cctggctaat gcaggaaaac gatctgcatg aaaaacagat tattcttctt accgggaatg
    35761 atagcgagcg agaagcgcag attgagcagc agttacgcga actaacgtta ctgccgctca
    35821 acattaaaca tatgtcggta aaggcatttt tgcagacagg cgctccgcgc ggcgcggcac
    35881 tgattattgc gccttatacc atgccgttac cgctcttttc accaccgctg atctatacgg
    35941 acctgacact gacaacacat caacaggagc agatccgcaa aatgctcgaa tcagcatgag
    36001 ggaacaactt tcgggcgcaa aaacattgcc ggaagcgcca ccagcgccat tacccagaac
    36061 acgccgtggc ccagatattg atacaggaaa ccggcgaaaa cggtcatgat agctatactg
    36121 ccgcccatcg cgacggcaga gtaaaccgcc tgtaaacgga tgacttcgct accctggcga
    36181 gcggcaatat agcgcatggc ggccaggtgg cagaccgtga aggcgccgca atgcagaatt
    36241 tgcaccacta tcaaccacgg caacgccgta gttgctccca taatgcccca gcgcactacg
    36301 ccgcaaatcg ccgagatcaa cagcatatcg cgtgcactac aacggcggaa aagtttatta
    36361 ctcagcgcaa agataatgac ttccgccacc acgcccagcg accacaaata ccccaccgcc
    36421 gaggccgagt agccagctgc ctgccagtaa atggcgctaa aaccgtaata ggccgcatgt
    36481 gccccctgca ataaacaaac gcaggccaga aagcgccagt tctgaagaac cagcgccaac
    36541 cacgctgacc aaccggtgct ctcctgctgg cggcttgccc cttgtggctg aatcgtcgga
    36601 cggatgagaa agccgagcag catagatgcc acgcccaatg ttaacagcgc gaggatcacc
    36661 tgataatcaa acatagtgac cagtttgccc gtcagcgccg agccaatgac aaacgccacc
    36721 gagccccaca gtcgcacttt gccgtaatca agcgggaact gcttttgcca cgtattcgcc
    36781 agtgcatcgg tcaacggtac cagcggtgag aaaaagaggt taaagccaat catcaccagc
    36841 atcagccacg ctacgtgcgc ccccgcccag aaggcgacag caaagagaag tgtcatcatt
    36901 gccagcacgc gcaaggcgga aatcaggcga gaaggatcgc tgacgcgggg cgcgatgagc
    36961 aaactcccga gaaaacgggc aaccagacct gcccccaata acaggccgat ggtttctggc
    37021 gttaaaccaa tccctttaag ccagacgctc cagaaaggta gaaaaatgcc gtaactaaaa
    37081 aagtatgtga aatagccgag cgccaaccag cgcgtggatt gcaaaaccat gagtccctcc
    37141 cgtcaggagg cgttagtctg gggttaatcg aatgttggtg caagttgaaa aataagaata
    37201 ttcgagcctg cttcccgatg tctgtaacgc cagttcacaa gcactaaaaa actgcgatcc
    37261 gcgacgcatg ttgcaaaaat aatgcgtaat tatgccgtta cgcttgccaa acgttcctgc
    37321 acaatggcga tgaaatcgcg taatgccggc ttcatctccc ctttcttcca cgccatcagt
    37381 aaagcaatgg aaggcacatt tccggcaatg ggacgaaaaa caacctgtcc ggtattaaaa
    37441 ttattcatat aaccgggtat caaagtgacg cccagcccca tgcccaccag attcatggtc
    37501 accagaatat tcgttgccac ctggacgata tttggctggc tgttttcttg cgcaaacctc
    37561 gctttaacga tcggcgcaag cgaaccggaa tacgccggat cggtactgac gaaattcacg
    37621 ccatccagtt gcgcggcggt gatctctttt tcatgtgcta aagggtgatc aaccggtaac
    37681 acaaccacta atggttcgtc aaaaagctcc agataatcaa tctccgggct ataaacagga
    37741 tggcgcatta agccgacgtc gagttcacca cggcgaattt tttcctcctg ttgcgtggtg
    37801 attaaactca ccagctcaat caaggtgtct ggctgtctga gacgaaacat cggtaatact
    37861 tttggcagta aattcacttc cgccgatggc acaaagccaa tggttaattg tctgtcttcc
    37921 tgaacagttt tccgcgcccg taatttggca ttttccgctt gttcgaggat tgccagcgcg
    37981 tcctggagaa aacattctcc cgccgccgtc aacgcgactt tgcgcttatc cctcaccagt
    38041 aacggaacac cgacacagtt ttcaagatcg cggatctggc tgcttaacga aggctgtgag
    38101 gtatgcagtt tttccgccgc acgggtaaag ttcagtgcct gcgccactgc gacgaaatag
    38161 cgtaaatgcc gtagttccat caccttcccc ttgttatcga aaaaacgtct cagctggtag
    38221 aaattaacta tttcacatat tagcaaccaa ccagcaacat ccttatggca caaaaataga
    38281 aggtcaatac atcttatctt tcaggattaa aaaatatgac cacaccctca gatttgaaca
    38341 tttaccaact gattgacacc caaaatggtc gggtcactcc gcgtatttat accgacccgg
    38401 atatttacca actggagctt gagcgtattt tcggtcgttg ctggctattt ctcgcccacg
    38461 aaagccagat cccaaaaccc ggtgatttct ttaacaccta catgggagaa gatgcggttg
    38521 tcgtagtgcg tcagaaagac ggcagcatca aggcgtttct caaccaatgc cgccaccggg
    38581 ccatgcgtgt gagttatgca gattgcggca acactcgcgc ctttacctgc ccgtatcacg
    38641 gctggtctta tggcattaac ggcgagttga tcgatgtacc gctggaacct cgcgcctacc
    38701 cacaagggtt gtgtaaatcc cactgggggc taaacgaagt tccttgtgtg gagagttata
    38761 aagggctgat ttttggcaac tgggatacca gcgcaccggg cctgcgtgat taccttggtg
    38821 acattgcctg gtatctggat ggcatgctgg atcgtcgcga aggcggcacc gaaattgtcg
    38881 gcggcgtaca aaagtgggtg atcaactgta actggaaatc cccggcagag cagttcgcca
    38941 gtgaccaata tcatgctctg ttcagccatg cttctgccgt tcaggtatta ggggcgaaag
    39001 atgatggcag cgataagcgt ctcggtgatg gacaaaccgc ccgcccggtg tgggaaaccg
    39061 ccaaagatgc gctgcaattt ggtcaggacg gtcacggtag cggtttcttc tttactgaaa
    39121 aaccggatgc taatgtctgg gtcgatggcg cggtttccag ctattaccgc gaaacctatg
    39181 ccgaagcaga acaacgttta ggtgaagttc gcgccctgcg cctggcgggt cataacaata
    39241 tattccccac gctttcatgg ctaaacggca ctgccacact ccgcgtctgg catccgcgcg
    39301 gccctgatca agttgaagtg tgggcgttct gtattactga caaagccgcc tccgatgaag
    39361 ttaaagccgc ttttgaaaac agcgccactc gtgcttttgg tcctgctggt tttctcgagc
    39421 aggatgactc ggagaactgg tgtgaaatcc agaaattgct taaaggccac cgcgcccgca
    39481 acagcaaact gtgtctggaa atggggcttg gtcaggaaaa gcgccgcgac gacggcattc
    39541 ctggcattac taactatatc ttttcagaaa ctgccgctcg tggaatgtac caacgctggg
    39601 ccgatcttct gagtagcgaa agctggcagg aagtgctcga taaaaccgcc gcttaccagc
    39661 aggaggtgat gaaatgagtg cgcaagtttc actagagtta catctccgca ttagccagtt
    39721 tctctttcac gaagccagct tactggacga ctggaaattt cgtgactggc tggcgcagct
    39781 cgacgaagag attcgttaca ccatgcgcac cacagttaac gcgcaaacac gcgaccgccg
    39841 caaaggcgtc cagccaccga caacctggat ttttaatgac accaaagacc agctggagcg
    39901 gcgaatcgcc cgtctggaaa cgggcatggc ctgggcagaa gagccgccgt cacgcacccg
    39961 tcacttaatc agcaactgcc agataagcga aaccgacatc ccaaacgtat ttgctgtgcg
    40021 ggtaaattat ctgctttatc aggcacaaaa agagcgcgat gaaacattct atgttggaac
    40081 gcgtttcgac aaagttcgcc gtctggaaga tgacaactgg cgcttgctgg aacgggatat
    40141 cgtcctggat caagcggtaa tcacttccca taacctgagt gtactgttct gatgaatcga
    40201 atttatgcgt gtcccgttgc ggatgtgccg gagggtgagg ctctccggat cgatacctcg
    40261 cccgtcatcg ccctgttcaa cgttggcggc gagttttatg ccattaacga tcgttgcagc
    40321 catggtaatg cgtcgatgtc agaagggtat ctggaagatg acgccacggt ggagtgcccg
    40381 ctacacgccg ccagtttttg cctgaaaacg gggaaagcgt tatgcctgcc cgccaccgat
    40441 ccgctcacca cttatccagt acacgttgaa ggtagtgaca ttttcatcga cttaccggag
    40501 gcgcagccat gagcgatctg cataacgagt ccatttttat taccggcggc ggatcgggat
    40561 tagggctggc gctggtcgag cgatttatcg aagaaggcgc gcaggttgcc acgctggaac
    40621 tgtcggcggc aaaagtcgcc agtctgcgtc agcgatttgg cgaacatatt ctggcggtgg
    40681 aaggtaacgt gacctgttat gccgattatc aacgcgcggt cgatcagatc ctgactcgtt
    40741 ccggcaagct ggattgtttt atcggcaatg caggcatctg ggatcacaat gcctcactgg
    40801 ttaatactcc cgcagagacg ctcgaaaccg gcttccacga gctgtttaac gtcaacgtac
    40861 tcggttacct gctgggcgca aaagcctgca ctccggcgtt aatcgccagt gaaggcagca
    40921 tgattttcac actgtcaaat gccgcctggt atcctggcgg cggtggcccg ctgtacaccg
    40981 ccagtaaaca tgccgcaacc ggacttattc gccaactggc ttatgaactg gcaccgaaag
    41041 tgcgggtgaa tggcgtcggc ccgtgtggta tggccagcga cctgcgcggc ccacaggcgc
    41101 tcgggcaaag tgaaacctcg ataatgcagt ctctgacgcc ggagaaaatt gccgccattt
    41161 taccgctgca atttttcccg caaccggcgg attttacggg gccgtatgtg atgttggcat
    41221 cgcggcgcaa taatcgcgca ttaagcggtg tgatgatcaa cgctgatgcg ggtttagcga
    41281 ttcgcggcat tcgccacgta gcggctgggc tggatcttta aggaagcacg atgaaagaaa
    41341 aaacgatcat tattgtcggt ggcgtgcaag cggcggcaat ggctgcggcc tcgctacgcc
    41401 agcaagggtt caccggtgag ctgcatctgt tttccgatga gcgacatctt ccttatgaac
    41461 gacctccact ctcgaaatcc atgttgctgg aagattcccc gcagttacag caggtgttac
    41521 ccgctaactg gtggcaggaa aacaatgttc atctgcattc cggtgtaacc atcaaaacgc
    41581 tgggtcgcga cacacgagag ttagtgttaa ccaacggcga aagctggcac tgggatcagc
    41641 tttttatagc aaccggcgcg gcagctcgac cgctgccgtt gcttgatgca ctgggagaac
    41701 gctgctttac cctacgccat gccggtgatg ccgccagact gcgagaagtt ctgcagcccg
    41761 aacggtcagt cgtgattgtc ggtgccggaa ctattggtct ggaactggct gccagcgcca
    41821 cgcagcgcag cgcagcgcag atgtaaggtg acagtgattg aactggcggc aaccgtcatg
    41881 ggccgtaatg caccaccgcc cgtgcaacgc tatcttttac agcgccacca gcaggctggt
    41941 gtgcgcattc tgctcaataa tgccattgaa catgtagtcg atggtgaaaa agtagaactg
    42001 acgctgcaaa gtggggaaac gcttcaggct gatgtggtga tttacggtat tggtatcagc
    42061 gccaacgagc aactggctcg cgaggccaac cttgatactg ccaatggcat tgtcattgat
    42121 gaggcttgcc gcacctgcga tcccgcgatc tttgccggtg gcgatgtggc aatcactcgt
    42181 cttgataatg gtgcactaca ccgctgtgaa agctgggaaa acgccaataa ccaggcgcaa
    42241 attgccgctg ccgcaatgtt agggctaccg ctaccgctac tgccgtggtt ctggagcgat
    42301 cagtacagtg ataacttaca gtttattggc gatatgcgtg gcgatgactg aggtagcctg
    42361 agtttaacgg acactccttc ctgaaatag
//
LOCUS       DAJVUJ010000011        40982 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.011, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000011 DAJVUJ010000000
VERSION     DAJVUJ010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40982
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.011"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(<1..334)
                     /locus_tag="OQQ48_000616"
     CDS             complement(<1..334)
                     /locus_tag="OQQ48_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCS1951494.1"
                     /translation="MNKEEQYLLFALSAPMEILNQGCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWHRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGE"
     gene            complement(415..1842)
                     /locus_tag="OQQ48_000617"
     CDS             complement(415..1842)
                     /locus_tag="OQQ48_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HCS1951495.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETEPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLDIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALILQWCRQHRVDYYPLVVMMIEARELVNDQGKQLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHLLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTEKQTVEPEPQPQPMPVDDGGPGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(1839..2546)
                     /locus_tag="OQQ48_000618"
                     /pseudo
     CDS             complement(1839..2546)
                     /locus_tag="OQQ48_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
     gene            2709..3686
                     /locus_tag="OQQ48_000619"
     CDS             2709..3686
                     /locus_tag="OQQ48_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCS1951496.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEHAIFWLKKTALQGHTFASN
                     ALGWILDRGEDPNYKEAVVWYQIAAESGMSYAQNNLGWMYRNGNGVAKDYALAFFWYK
                     QAALQGHSDAQNNLADLYEDGKGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEDLL"
     gene            complement(3756..4238)
                     /locus_tag="OQQ48_000620"
     CDS             complement(3756..4238)
                     /locus_tag="OQQ48_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HCS1951497.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            4473..7055
                     /gene="leuS"
                     /locus_tag="OQQ48_000621"
     CDS             4473..7055
                     /gene="leuS"
                     /locus_tag="OQQ48_000621"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCS1951498.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKS
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            7070..7651
                     /gene="lptE"
                     /locus_tag="OQQ48_000622"
     CDS             7070..7651
                     /gene="lptE"
                     /locus_tag="OQQ48_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HCS1951499.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            7651..8682
                     /gene="holA"
                     /locus_tag="OQQ48_000623"
     CDS             7651..8682
                     /gene="holA"
                     /locus_tag="OQQ48_000623"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCS1951500.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRTLHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQTQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            8684..9325
                     /gene="nadD"
                     /locus_tag="OQQ48_000624"
     CDS             8684..9325
                     /gene="nadD"
                     /locus_tag="OQQ48_000624"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HCS1951501.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIANKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNQED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            9349..9959
                     /locus_tag="OQQ48_000625"
                     /pseudo
     CDS             9349..9959
                     /locus_tag="OQQ48_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415171.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
     gene            10219..10536
                     /gene="rsfS"
                     /locus_tag="OQQ48_000626"
                     /gene_synonym="rsfA"
     CDS             10219..10536
                     /gene="rsfS"
                     /locus_tag="OQQ48_000626"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCS1951502.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            10540..11007
                     /gene="rlmH"
                     /locus_tag="OQQ48_000627"
     CDS             10540..11007
                     /gene="rlmH"
                     /locus_tag="OQQ48_000627"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCS1951503.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            11038..12939
                     /gene="mrdA"
                     /locus_tag="OQQ48_000628"
     CDS             11038..12939
                     /gene="mrdA"
                     /locus_tag="OQQ48_000628"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HCS1951504.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTTHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            12942..14054
                     /gene="mrdB"
                     /locus_tag="OQQ48_000629"
                     /gene_synonym="rodA"
     CDS             12942..14054
                     /gene="mrdB"
                     /locus_tag="OQQ48_000629"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HCS1951505.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            14065..15153
                     /gene="rlpA"
                     /locus_tag="OQQ48_000630"
     CDS             14065..15153
                     /gene="rlpA"
                     /locus_tag="OQQ48_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HCS1951506.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            15293..16504
                     /gene="dacA"
                     /locus_tag="OQQ48_000631"
     CDS             15293..16504
                     /gene="dacA"
                     /locus_tag="OQQ48_000631"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HCS1951507.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDMYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            16615..16878
                     /locus_tag="OQQ48_000632"
     CDS             16615..16878
                     /locus_tag="OQQ48_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HCS1951508.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVEILYEELGKIDIVRMVL"
     gene            16979..17620
                     /gene="lipB"
                     /locus_tag="OQQ48_000633"
     CDS             16979..17620
                     /gene="lipB"
                     /locus_tag="OQQ48_000633"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HCS1951509.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            17879..18832
                     /locus_tag="OQQ48_000634"
     CDS             17879..18832
                     /locus_tag="OQQ48_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCS1951510.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIALNLLTIFEAVYVHK
                     GIVNAAKVLNLPPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEEQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNKEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            19041..20006
                     /gene="lipA"
                     /locus_tag="OQQ48_000635"
     CDS             19041..20006
                     /gene="lipA"
                     /locus_tag="OQQ48_000635"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HCS1951511.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVAIEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(20107..20310)
                     /gene="tatE"
                     /locus_tag="OQQ48_000636"
     CDS             complement(20107..20310)
                     /gene="tatE"
                     /locus_tag="OQQ48_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HCS1951512.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(20439..21227)
                     /locus_tag="OQQ48_000637"
     CDS             complement(20439..21227)
                     /locus_tag="OQQ48_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HCS1951513.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            21320..21703
                     /gene="crcB"
                     /locus_tag="OQQ48_000638"
     CDS             21320..21703
                     /gene="crcB"
                     /locus_tag="OQQ48_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HCS1951514.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLTANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(21758..21967)
                     /gene="cspE"
                     /locus_tag="OQQ48_000639"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(21758..21967)
                     /gene="cspE"
                     /locus_tag="OQQ48_000639"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCS1951515.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(22142..22702)
                     /gene="pagP"
                     /locus_tag="OQQ48_000640"
     CDS             complement(22142..22702)
                     /gene="pagP"
                     /locus_tag="OQQ48_000640"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HCS1951516.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADEWMTTFRENIAPTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            23291..24676
                     /gene="dcuC"
                     /locus_tag="OQQ48_000641"
     CDS             23291..24676
                     /gene="dcuC"
                     /locus_tag="OQQ48_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCS1951517.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(24717..25397)
                     /gene="dpiA"
                     /locus_tag="OQQ48_000642"
                     /gene_synonym="citB"
     CDS             complement(24717..25397)
                     /gene="dpiA"
                     /locus_tag="OQQ48_000642"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HCS1951518.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTSARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(25366..27023)
                     /gene="dpiB"
                     /locus_tag="OQQ48_000643"
                     /pseudo
     CDS             complement(25366..27023)
                     /gene="dpiB"
                     /locus_tag="OQQ48_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
     gene            27372..28430
                     /gene="citC"
                     /locus_tag="OQQ48_000644"
     CDS             27372..28430
                     /gene="citC"
                     /locus_tag="OQQ48_000644"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HCS1951519.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLKTPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            28445..28741
                     /gene="citD"
                     /locus_tag="OQQ48_000645"
     CDS             28445..28741
                     /gene="citD"
                     /locus_tag="OQQ48_000645"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HCS1951520.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGTLDCILRARLEALLARASGIPTLPWEDCQ"
     gene            28738..29646
                     /gene="citE"
                     /locus_tag="OQQ48_000646"
     CDS             28738..29646
                     /gene="citE"
                     /locus_tag="OQQ48_000646"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HCS1951521.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            29657..31189
                     /gene="citF"
                     /locus_tag="OQQ48_000647"
     CDS             29657..31189
                     /gene="citF"
                     /locus_tag="OQQ48_000647"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HCS1951522.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIHRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQEQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            31193..31743
                     /gene="citX"
                     /locus_tag="OQQ48_000648"
                     /pseudo
     CDS             31193..31743
                     /gene="citX"
                     /locus_tag="OQQ48_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
     gene            31718..32595
                     /gene="citG"
                     /locus_tag="OQQ48_000649"
                     /pseudo
     CDS             31718..32595
                     /gene="citG"
                     /locus_tag="OQQ48_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
     gene            32646..34109
                     /gene="citT"
                     /locus_tag="OQQ48_000650"
     CDS             32646..34109
                     /gene="citT"
                     /locus_tag="OQQ48_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HCS1951523.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPNGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            34220..35026
                     /gene="rna"
                     /locus_tag="OQQ48_000651"
     CDS             34220..35026
                     /gene="rna"
                     /locus_tag="OQQ48_000651"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HCS1951524.1"
                     /translation="MKEFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEAHASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSRGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPSNCGKTFVIDKVGY"
     gene            35256..35666
                     /gene="rnk"
                     /locus_tag="OQQ48_000652"
     CDS             35256..35666
                     /gene="rnk"
                     /locus_tag="OQQ48_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HCS1951525.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(35897..37135)
                     /locus_tag="OQQ48_000653"
     CDS             complement(35897..37135)
                     /locus_tag="OQQ48_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
                     /protein_id="HCS1951526.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRMQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLSFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEEAQKAVSGLVNAMPGGTI"
     gene            37356..37784
                     /gene="uspG"
                     /locus_tag="OQQ48_000654"
     CDS             37356..37784
                     /gene="uspG"
                     /locus_tag="OQQ48_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HCS1951527.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLTQDDGVIHLLHVLPGSA
                     SLSLDRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(37905..39470)
                     /gene="ahpF"
                     /locus_tag="OQQ48_000655"
     CDS             complement(37905..39470)
                     /gene="ahpF"
                     /locus_tag="OQQ48_000655"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCS1951528.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNLGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(39641..40204)
                     /gene="ahpC"
                     /locus_tag="OQQ48_000656"
     CDS             complement(39641..40204)
                     /gene="ahpC"
                     /locus_tag="OQQ48_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HCS1951529.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            40576..>40982
                     /gene="dsbG"
                     /locus_tag="OQQ48_000657"
     CDS             40576..>40982
                     /gene="dsbG"
                     /locus_tag="OQQ48_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HCS1951530.1"
                     /translation="MLKKILLLALLPAIAFAEELPAPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQA"
BASE COUNT        10182 a        10481 c        10069 g        10250 t
ORIGIN      
        1 cttccccaca gcacatagcg gtttgggcaa cgaattgggt ataaatcaac cctctctccg
       61 agccaccagc aattaatgct ttccactctt caggggaaga gcgatgccat gtcagatata
      121 agccagctaa atcattggca tgaccatcat cagtcatctg ataaatagtc tgaattaaat
      181 catcacgatt atttattccc caggatgatg agagatcgaa ttcttttata ccggtataca
      241 tttttggcga gtcatgcgcg ggtttgcaac cctgattaag tatttccatg ggcgcagaaa
      301 gcgcaaaaag aagatattgt tcttccttat tcatttgtta ataatccctt aagtgcatta
      361 actgactact tttttatatt acttgccggg ctttccgcac attaacgtcg actgtcaccc
      421 gaacagatgg agtattttcc ctatcaaacc ggcaaagata aaaatgtaga aaataatttt
      481 gatgacgccc agacagcccg gcccaccatc gtcaacgggc attggctgcg gttgcggctc
      541 cggttcaaca gtttgcttct cggtaaaatt cgccgcctga attaataatc gacgaataat
      601 taatgcttcc agtggacaac gatcttctgc atcaagacgg cttacgatcg gcattaatga
      661 ttcttctgtc ggttggctgg cctgcactaa ttgtttggca actaaatata atggccataa
      721 gagatgttct gtatcacgcg tgagtgagat ccgcggctta cgtcctttgt gcaaaaccat
      781 ctcgaccagt gaacgcccca gcgcagagag tttttcatcg ctgagtattt cgtataaatg
      841 aaaacgcgta cgggcgctgt cgccggggac ataaagtagc tgtttaccct gatcgttaac
      901 cagttcacga gcttcaatca tcatcaccac taacgggtaa taatcgaccc gatgctgacg
      961 gcaccattgc aatattaaag catccgcctg ggtggaggga tactcgcgcc agtagtcaca
     1021 aagttctgcc agcaagcttt cgccttctcc acaatagacg cgttgcgcgt attcataata
     1081 acgcgcatct tcattatcag gttcagcttc ccgccacgac cgggcgtaat ccagaagttc
     1141 ggggatatcc aggcgacctg cgctatacca gcggagtaaa tttctgtgca gttttgcatc
     1201 atcggggatg atcactgcgc gatgttgcgc taaatacaat gtcagccatt ctggatgcga
     1261 tgaccaaatc cgagcgaaca tttggtaaaa atcgatggtg tcattttgca cggcaacggg
     1321 cagatgcaga atggtctgga agttaaaaac attccccttc gcaatggcat ataaaaagga
     1381 ttccagttct tcttcgtcta tttccccgac gttttcctcc tgcaaccacc gcaatctttc
     1441 tgctaataac accacgcact cgaaggataa atgggcagtg ttcatggcga ttgtgcacag
     1501 cgaccagcgt aattcatcaa tctcttccat cgagcaataa tttaattgct gaatgaatcg
     1561 ctgccaggcg gagggcagaa aacgttcgct atcagaggca agtaacgcgc gaaaggcgag
     1621 cagaatttca tgttctgcta cctcatattc ttctggttgc caaacagatt tagcaggcga
     1681 ctgcgcaatc cgtagcgcct cctcatacgc ctgacgaagt tgtttaaaac cctgcggttc
     1741 ggtttctgga tgaaaggacg gtaacagcgc cagataagcg cggcggataa tatcgacgtc
     1801 agtcgtttcc tctatatcga ggatcttcca gcaatttttc ataagtcgct catatcctta
     1861 agcgtttccg ggcatgtggg gtagtcagcc agtggttgcc aggacaaggt agggtaatac
     1921 gcatcatcac tggcaaataa ttgataaaac atatcattgc cggaatcgtc gtattcttta
     1981 cgcgctaaga attcgcgtaa taaaggcaga tgatcatctt cgggagactg ccaatattgc
     2041 cgaccaaagg tataaccggc aaagtattgc cgccagttac tataataacg tagagcccgt
     2101 agatgaatac gcgactggat ccatagactt tcttcttcac tcaaccagtt attaagtacg
     2161 cccattcgac ttaaaaaacc catccggaca taatcccatg ctttaattcc tccctcacca
     2221 cagcattcgg ctgtgctggc gacaaattgc gcataagcct gaccttgctc atttaattca
     2281 gcaagatagt cacgccattc acatggagaa taacgaaacc agcggtgata aaaccctgcc
     2341 aaccgagcgg catgaccgtt ctcgatcatt cggtgaattg tgcgcagcag gtcatcacgg
     2401 tcatcaattc cccattagga agaaaggtca aaaaagcatg tacctaaata cattttgggg
     2461 gatgaatgcg aaggtaaaca gcattcattg cgtatttcca tcggagtgga aagtgcaaac
     2521 aatatttttt gcgaccccat gtccatataa tttatcctta aacattcatt taatgaatgt
     2581 aaactacatt tcgagactca gttatttata cttttctgac gcgacataat acgcataaaa
     2641 taaggcttac gcaaaaatgg aacacataaa atataatatg ttttatctac aaccataagg
     2701 aaatgataat gatttttacg tcaagttgct gcgataattt atcaatagat gagattatcg
     2761 aacgtgctga aaaaggcgat tgcgaggctc aatatattgt tgggttttat tataatcgcg
     2821 atagcgcaat tgactctccg gacgacgaaa aggcctttta ctggctgaag ctggccgccg
     2881 agcaaggtca ttgtgaagca caatattcct taggacaaaa gtatactgag gataaaagcc
     2941 gtcataaaga taatgagcat gccatctttt ggctgaaaaa aactgcccta caaggccata
     3001 ctttcgcttc caacgccctt ggctggatac tggatcgtgg agaagacccc aattataaag
     3061 aagcggttgt ctggtatcag atagccgcgg agagcggaat gtcttatgcg caaaataatc
     3121 ttgggtggat gtacagaaat ggcaacggag tcgcaaaaga ctatgcgctg gcatttttct
     3181 ggtacaaaca agctgcatta caaggccata gtgacgcgca aaacaatctg gccgatcttt
     3241 atgaagacgg aaaaggcgtt gctcaaaaca agacactcgc cgcattctgg tatttgaaaa
     3301 gcgcacagca gggtaatcgg cacgcccagt ttcaaattgc atgggattat aacgctggcg
     3361 aaggggtgga ccaggactat aagcaagcga tgtactggta tctgaaggct gccgctcagg
     3421 aaagcgtcgg cgcttacgtc aacatcggtt atatgtataa acacggacaa ggcgttgaga
     3481 aagattatca ggctgccttt gaatggttta cgaaagccgc tgaatgcaat gacgccactg
     3541 cctggtataa cctggccatt atgtatcact acggagaagg aagacctgtc gatctccgac
     3601 aggctctcga cctgtatcgt aaagttcagt catccggaac cagagatgtc agtcaggaaa
     3661 ttcgtgagac tgaagattta ctgtagagat aaaaacaagg gccggggaat ttcattcggc
     3721 ccatattctc tttattgctg tgcgaaatca gactattacg gctcaaacgg gcgtctctgg
     3781 aactggtcat gaccacattt cgggcatagc gtcagcactt ccggtgtgta gatcgggaga
     3841 tggaagtgac atttctcgca gaccagattt cccagcccga ccacttctcc gctgtgataa
     3901 accccatgat gattgaggtc ctggaaaact tcgcgccatt caagctgcgt tttatcggtg
     3961 atatctgcca gctcctgcca taagctttct ttaatcaccc gcataaagac gctgtcagat
     4021 tcttctttca ggctctcttc atagctcatg gcgaactctt ccaggtcacg tcttacagct
     4081 cgcgtcagct catcgacctc ggttcgcgtt aactcccctg tttttattac gcgctcgcgc
     4141 gcctgttcca ccagtgcgtc gatatcacgt tcgccattgc gcaggcgttc gctcagtgac
     4201 gcgaccagtt cacggtaata ttgagcaacc ttgttcatcg tttcatctcc cgggtcgtta
     4261 actgactata ataatagacg ttctttgttc cttgctttgc taatacgccc acaaactctg
     4321 taaattaatg tatgaataat ccgggcatca tttcaacgtc gagtgcgcga aaggctgttt
     4381 tgacgcggac gtttgggcta tgctatgcgg atctgaaaaa ccacctcaac gctacatttg
     4441 tagccgtatt gaaaacagga ccactggctg ccatgcaaga gcaataccgc ccggaagaga
     4501 tagaatccaa agtacagctt cattgggatg agaagcgcac atttgaagta accgaagacg
     4561 agagcaaaga gaagtattac tgcctgtcta tgcttcccta tccttctggt cgactacaca
     4621 tgggccacgt acgtaactac accatcggtg acgtgatcgc ccgctaccag cgtatgctgg
     4681 gcaaaaacgt cctgcagccg atcggctggg acgcgtttgg tctgcctgcg gaaggcgcgg
     4741 cggtgaaaaa caacaccgct ccggcaccgt ggacgtacga caacatcgcg tatatgaaaa
     4801 accagctcaa aatgctgggc tttggttatg actggagccg cgagctggca acctgtacgc
     4861 cggaatacta ccgttgggaa cagaaattct tcaccgagct gtataaaaaa ggcctggtat
     4921 ataagaagac ttctgcggtc aactggtgcc cgaacgacca gaccgtactg gcgaacgaac
     4981 aagttatcga cggctgctgc tggcgctgcg ataccaaagt tgaacgtaaa gagatcccgc
     5041 agtggtttat caaaatcact gcttacgctg acgagctgct caacgatctg gataaactgg
     5101 atcactggcc agacaccgtt aaaagcatgc agcgtaactg gatcggtcgt tccgaaggcg
     5161 tggagatcac cttcaacgtt aacgactatg acaacacgct gaccgtttac actacccgcc
     5221 cggacacctt tatgggttgt acctacctgg cggtagctgc gggtcatccg ctggcgcaga
     5281 aagcggcgga aaataatcct gaactggcgg cctttattga cgaatgccgt aacaccaaag
     5341 ttgccgaagc tgaaatggcg acgatggaga aaaaaggcgt cgatactggc tttaaagctg
     5401 ttcacccatt aacgggcgaa gaaattcccg tttgggcagc aaacttcgta ttgatggagt
     5461 acggcacggg cgcagttatg gcggttccgg gtcacgacca gcgcgactac gagtttgcct
     5521 ctaaatacgg cctgaacatc aaaccagtta tcctggctgc tgacggctct gagccagatc
     5581 tttctcagca agccctgact gaaaaaggcg tgctgttcaa ctctggcgaa ttcaacggtc
     5641 ttgaccatga agcggccttc aacgccatcg ccgataaact gactgcgatg ggcgttggcg
     5701 agcgtaaagt gaactaccgc ctgcgcgact ggggtgtttc tcgtcagcgt tactggggcg
     5761 cgccgattcc gatggtgacg ctggaagacg gtaccgtaat gccgaccccg gacgaccagc
     5821 tgccggtgat cctgccggaa gatgtggtga tggacggcat taccagcccg attaaagcag
     5881 atccggagtg ggcaaaaact accgttaacg gtatgccagc gctgcgtgaa accgacactt
     5941 tcgacacctt tatggagtcc tcctggtact atgcgcgcta cacttgccca gagtacaaag
     6001 aaggtatgct ggattccgaa gcggctaact actggctgcc ggtggatatc tacattggtg
     6061 gtatcgaaca cgccattatg cacctgctct acttccgctt cttccacaaa ctgatgcgtg
     6121 atgcaggcat ggtgaactct gacgaaccag cgaaacagtt gctgtgtcag ggtatggtgc
     6181 tggcagatgc cttctactat gttggcgaaa acggcgaacg taactgggtt tccccggttg
     6241 atgctatcgt tgaacgtgac gagaaaggcc gtatcgtgaa agcgaaagat gcggcaggcc
     6301 atgaactggt ttataccggc atgagcaaaa tgtccaagtc gaagaacaac ggtatcgacc
     6361 cacaggtgat ggttgaacgt tacggcgcgg acaccgttcg tctgtttatg atgtttgctt
     6421 ctccggctga tatgactcta gaatggcagg aatccggtgt ggaaggggct aaccgcttcc
     6481 tgaaacgtgt ctggaaactg gtttacgagc acacagcaaa aggtgatgtt gcggcactga
     6541 acgttgatgc gctgactgaa gatcagaaag cgctgcgtcg cgatgtgcat aaaacgatcg
     6601 ctaaagtgac cgatgatatc ggccgtcgtc agaccttcaa caccgcaatt gcggcgatta
     6661 tggagctgat gaacaaactg gcgaaagcac caaccgatgg cgagcaggat cgcgctctga
     6721 tgcaggaagc gctgctggcc gttgtccgta tgcttaaccc gttcaccccg cacatctgct
     6781 tcacgctgtg gcaggaactg aaaggcgaag gcgatatcga caacgcgccg tggccggttg
     6841 ctgacgaaaa agcgatggtg gaagactcca cgctggtcgt ggtgcaggtt aacggtaaag
     6901 tccgtgccaa aatcaccgtt ccggtggacg caacggaaga acaggttcgc gaacgtgctg
     6961 gccaagaaca tctggtagca aaatatcttg atggcgttac cgtgcgtaaa gtgatttacg
     7021 taccaggtaa actcctcaat ctggtcgttg gctaagcgcg ggaggaagcg tgcgatatct
     7081 ggcaacattg ttgttatctc tggcggtgtt aatcaccgcc gggtgtggct ggcatctgcg
     7141 tgataccacg caggttcctt ccactatgaa ggtcatgatc ctggactcag gcgatccgaa
     7201 cgggccatta agccgtgcgg tgcgtaacca gttacgtctg aatggtgtcg agttgcttga
     7261 taaagaaacc acgcgtaagg acgttccatc cctgcgtttg ggtaaagtga gcatcgcgaa
     7321 agataccgca tcggtattcc gtaacggtca aacagcagag tatcagatga tcatgacggt
     7381 gaatgcgacc gtgttgatcc ccggccgtga tatctacccg attagcgcca aagtcttccg
     7441 ttcgttcttt gataacccgc aaatggcgtt agcgaaagat aacgaacaag acatgatcgt
     7501 aaaagagatg tacgaccgtg ctgccgaaca gctgattcgt aagctgccaa gcatccgtgc
     7561 tgcggatatt cgttccgacg aagaacagac gtcgaccaca acggatactc cggcaacgcc
     7621 tgcacgcgtc tccaccacgc tgggtaactg atgattcggt tgtacccgga acaactccgc
     7681 gcgcagctca atgaagggct gcgcgcggcg tatcttttac ttggtaacga tcctctgtta
     7741 ttgcaggaaa gccaggacgc tgttcgtcag gtagctgcgg cacaaggatt cgaagaacac
     7801 cacacttttt ccattgatcc caacactgac tggaatgcga tcttttcgtt atgccaggct
     7861 atgagtctgt ttgccagtcg acaaacgcta ttgctgttgt taccagaaaa cggaccgaat
     7921 gcggcgatca atgaacaact tctcacactc accggacttc tgcatgacga cctgctgttg
     7981 atcgtccgcg gtaataaatt aagcaaagcg caagaaaatg ccgcctggtt tactgcgctt
     8041 gcgaatcgca gcgtgcaggt gacctgtcag acaccggagc aggctcagct tccccgctgg
     8101 gttgctgcgc gcgcaaaaca gctcaactta gaactggatg acgcggcaaa tcaggtgctc
     8161 tgctactgtt atgaaggtaa cctgctggcg ctggctcagg cactggagcg tttatcgctg
     8221 ctctggccag acggcaaatt gacattaccg cgcgttgaac aggcggtgaa tgatgccgcg
     8281 catttcaccc cttttcattg ggttgatgct ttgttgatgg gaaaaagtaa gcgcacattg
     8341 catattcttc agcaactgcg tctggaaggc agcgaaccgg ttattttgtt gcgcacatta
     8401 caacgtgaac tgttgttact ggttaacctg aaacgccagt ctgcccatac gccactgcgt
     8461 gcgttgtttg ataagcatcg ggtatggcag aaccgccggg gcatgatggg cgaggcgtta
     8521 aatcgcttaa gtcagacgca gttacgtcag gccgtgcaac tcctgacacg aacggaactc
     8581 accctcaaac aagattacgg tcagtcagtg tgggcagagc tggaagggtt atctcttctg
     8641 ttgtgccata aacccctggc ggacgtattt atcgacggtt gatatgaaat ctttacaggc
     8701 tctgtttggc ggcacctttg atccggtgca ctatggtcat ctaaaacccg tggaaacgct
     8761 ggcgaatttg attggtctga cgcgggtcac aatcatccct aataatgttc ctccgcatcg
     8821 tccccagccg gaagcgaaca gcgtgcagcg taaacacatg cttgaactgg cgattgccaa
     8881 caagccatta tttactcttg atgaacgcga gctaaagcgc aatgccccct cttacactgc
     8941 gcaaacactg aaagagtggc ggcaggaaca aggaccggac gtgccgctgg cgtttattat
     9001 tggtcaggat tcactgctga cctttccgac ctggtacgaa tacgaaacga tactcgacaa
     9061 tgcacatttg atcgtctgtc ggcgtccagg ttacccactt gaaatggcgc aaccgcaata
     9121 ccagcaatgg ctggaagatc atttgacaca taaccaggaa gatcttcacc ttcagcctgc
     9181 cggtaaaatt tatctggctg aaacgccgtg gtttaacatc tcggcgacca tcatccgcga
     9241 acgtttgcaa aacggtgaat cgtgtgagga tttattgccg gaaccggtac tgacttacat
     9301 taaccaacaa ggcttgtatc gctgatacct gtcgttacgg agggcatgat gcgactgtgg
     9361 ttaattcgtc atggtgaaac gcaagcgaat atcgatggtc tttacagcgg tcatgcgccc
     9421 cccccctgac cgcgcgcggt attgagcaag cgcaaaatct gcatacgctg ctacatggtg
     9481 tttcctttga tctggtttta tgcagtgaac tggaacgggc acagcatacc gcgcgactgg
     9541 ttctcagtga ccgccagctc cccgtgcaaa tcatccctga actcaacgaa atgttttttg
     9601 gcgactggga gatgcgacat catcgcgacc tcatgcaaga agatgccgaa aactatagcg
     9661 cgtggtgcaa tgactggcag catgcaatcc ccacgaacgg tgaaggattt caggcatttt
     9721 cgcaacgtgt ggaacgcttt atcgcaaggc ttagtgaatt tcagcactat cagaatattt
     9781 tagtcgtcag ccatcagggt gtactgagtc tgttaatcgc ccgtttaatt ggcatgcctg
     9841 ccgaggctat gtggcatttt cgcgttgacc aggggtgttg gagcgccatt gatatcaacc
     9901 aaaaattcgc gacgctacgc gtcctcaata gccgtgccat cggggtcgat aatgcatgac
     9961 ttttctgttt ttttacgggt aagccgcaac gaccattgac agacccgggc aggctgatat
    10021 tctccgcagc cagacttttt tcgccagaca cgactttgta gaaattgttt tacaaaaatg
    10081 gcgatgcaat ctgcggcgcg gggtgggatg atagcccact ttcgaaagcc gattcggcga
    10141 caattgtccc gaaatcgcct ctggttcagg tatactgaca gaccattttt atctatttga
    10201 ttcacccagg gggaaaactt gcagggtaaa gcactccagg attttgttat cgacaaaatt
    10261 gatgacctca aaggtcagga catcatcgcc ttagacgttc aaggcaaatc cagcatcacc
    10321 gactgcatga tcatctgtac gggtacgtcc agccgtcatg ttatgtccat tgctgaccac
    10381 gttgtgcagg agtctcgcgc agcgggcctg ttaccgctcg gcgtagaagg tgaaaacagc
    10441 gccgactgga ttgtcgtgga tttgggcgat gtgattgtcc atgtcatgca ggaagagagc
    10501 cgtcgcctgt atgaactgga aaaactctgg agttaatgcg tgaagctgca acttgtcgcc
    10561 gtgggaacga aaatgccgga ctgggtacaa accggtttta ccgagtacct gcgtcgtttt
    10621 ccgaaagata tgcccttcga gctgattgaa attccggccg gaaaacgcgg caagaatgcg
    10681 gacatcaagc gcatactcga caaagagggt gagcagatgt tggcggccgc aggcaaaaac
    10741 cgcattgtca ccctcgatat tccaggcaag ccctgggata cgccgcagtt agccgctgag
    10801 ctggaacgct ggaagctgga tggtcgcgac gtcagtctac tgattggcgg gcctgaaggg
    10861 ttgtcgcctg cctgtaaagc ggcggcggag cagagctggt cgctgtcggc gcttaccctc
    10921 ccccatccgc tggttcgcgt gctggtcgca gagagtctgt accgggcgtg gagcatcacc
    10981 accaaccatc cttatcaccg tgagtgataa gggagctttg agtagaaaac gcagcggatg
    11041 aaactacaga actcttttcg cgactatacg gcagagtccg cgctgtttgt gcgccgggcg
    11101 ctggtcgcct ttttggggat tttgctgctg accggcgtgc ttatcgccaa cctgtataat
    11161 ctgcaaattg ttcgctttac cgactaccag acccgctcta atgaaaaccg cattaagctg
    11221 gtgcctatcg cgcccagccg cggcattatt tacgaccgta atggtatccc tctggccctc
    11281 aaccgcacta tctaccagat agaaatgatg ccagagaaag tcgataacgt gcagcaaacg
    11341 ctggacgctt tgcgcagcgt ggttgatctg accgatgacg atattgctgc attccgaaaa
    11401 gagcgcgcac gttcacaccg tttcacctct attcccgtga aaactaacct gaccgaagta
    11461 caagtagctc gctttgccgt caatcagtac cgttttccgg gggttgaagt taaaggctat
    11521 aaacgtcgtt actatcctta cggttcggcg ttgacccacg tcatcggcta tgtgtcgaaa
    11581 atcaacgata aagacgtcga acgcctgaat aatgacggca aactggccaa ctatgcggca
    11641 acgcatgata tcggtaagct gggcattgag cgttactatg aagacgtgct gcacggtcag
    11701 accggttatg aagaggttga agttaacaac cgtgggcgtg ttatccgcca gttaaaagaa
    11761 gtaccaccgc aagccggaca cgatatttac ctgacgctgg atctcaaact ccagcaatat
    11821 attgaaacgc tgctggcggg tagccgcgca gctgtggtag tcaccgatcc gcgtacaggt
    11881 ggggtgctgg cgctggtttc cacgcctagt tatgacccaa acttgtttgt tgacggtatc
    11941 tccagcaaag attattccgc cttgttgaac gacccgaata caccgctggt gaaccgcgcc
    12001 acacaggggg tttatcctcc cgcgtctaca gttaaaccct atgtggcggt ttcggcattg
    12061 agcgccgggg tgatcacgcg caatacgacg ctgtttgacc caggctggtg gcaactgcca
    12121 ggttcggaaa aacgttatcg tgactggaaa aaatggggcc acgggcgtct gaatgtcaca
    12181 agatcgctgg aagaatctgc ggataccttc ttctatcagg tggcctacga tatggggatc
    12241 gatcgcctct ccgaatggat gggtaaattc ggttatggtc attacaccgg tatcgacctg
    12301 gcggaagaac gttccggcaa catgcctacc cgcgaatgga aacagaaacg ctttaaaaaa
    12361 ccgtggtatc agggtgacac cattccggtt ggtatcggtc agggttactg gacagcgacc
    12421 ccaatccaga tgagtaaggc actgatgatc ctgattaatg acggtatcgt gaaggttccg
    12481 catttgctga tgagcaccgc cgaagacggc aaacaggtgc catgggtaca accgcatgaa
    12541 ccgcccgtcg gcgatattca ttccggttac tgggagctgg cgaaagacgg tatgtacggt
    12601 gttgctaacc gccctaacgg tacgacgcat aaatactttg ccagcgcacc gtacaaaatt
    12661 gcggcgaaat ctggtaccgc tcaggtcttc ggtctgaaag cgaacgaaac ctataatgcg
    12721 cacaaaattg ccgagcgttt acgtgaccac aaactgatga ccgcctttgc gccatacaac
    12781 aatccgcaag tggctgtcgc catgattctg gagaacggcg gcgcgggtcc ggcggttggt
    12841 acgctgatgc gtcagatcct cgaccacatt atgctgggtg ataacaacac cgatctgcct
    12901 gcggaaaatc cagcggttgc cgcagcggag gaccattaat catgacggat aatccgaata
    12961 aaaaaacatt ctgggataaa gtccatctcg atcccacaat gctgctgatc ttactggcat
    13021 tgctggttta cagcgcccta gttatctgga gcgccagcgg tcaggatatt ggcatgatgg
    13081 agcgtaaaat cggccaaatc gcgatgggtc tggtcatcat ggtggtgatg gcgcaaattc
    13141 ctccacgcgt ttatgaaggc tgggccccct atctctatat catctgtatt attttgctgg
    13201 tggcggtaga tgctttcggt gccatctcta aaggtgctca acgctggctg gacctcggta
    13261 ttgttcgttt tcagccgtcg gaaattgcca aaatagccgt accactgatg gttgcgcgct
    13321 ttatcaaccg cgacgtttgc ccaccgtcgt tgaagaacac aggcatcgcg ctggtgctga
    13381 tatttatgcc cacgctgctg gtggctgcac agcctgacct gggaacatca atcctcgttg
    13441 cgctttccgg tctgtttgta ctgttcctct ctggccttag ctggcgtctg attggcgtcg
    13501 cagtagtgct ggtggcggcg ttcattccta ttctgtggtt cttcctgatg catgattacc
    13561 agcgccagcg cgtaatgatg ctcctagacc cggaatcaga cccactcggc gcgggctatc
    13621 acattattca gtctaaaatt gctattggct ccggcggatt acgcggcaaa ggctggctgc
    13681 atggcactca gtcacaactt gaatttctac ccgaacgcca taccgacttt atcttcgcgg
    13741 tactggcgga agagctggga ttagtgggca ttttgattct gctcgctctc tacattctgc
    13801 tgatcatgcg cgggctgtgg atagccgcca gagcgcaaac cacctttggt cgcgtcatgg
    13861 ctggcggctt aatgctgata ttattcgttt atgtcttcgt aaatattggt atggtaagcg
    13921 gtattctgcc ggttgtaggg gttccgctcc cactggtcag ttatggagga tcagcgctaa
    13981 ttgtgctgat ggctgggttc gggattgtaa tgtcaatcca cacccacagg aaaatgttgt
    14041 cgaaaagcgt gtaagaggtg cgcaatgcgt aagcagtggc tcgggatctg catcgcggca
    14101 ggaatgctcg cggcatgtac aagcgatgat ggtcagcaac agacggtaag tgtaccgcag
    14161 cctgcggtat gtaacggccc catagttgaa attagcgggg cggacccgcg tttcgaacca
    14221 ctgaacgcga cggcaaatca ggattaccag cgcgacggta aaagctacaa aatcgtgcag
    14281 gatccgtctc gatttagcca ggcgggactg gcggcaatct atgatgccga accaggcagt
    14341 aacctgacgg cctctggcga agctttcgat ccgacacagc tgacggcggc ccatccaacg
    14401 cttccgatcc ccagctacgc cagaatcact aacctggcta acgggcgaat gatcgtggtg
    14461 cgcattaatg atcgcggccc ttacggcaac gaccgcgtta tttcgctttc tcgcgcggca
    14521 gctgaccgtc ttaacacgtc aaacaacacc aaagttcgta tcgatccgat tattgtcgcc
    14581 caggatggtt cgctttctgg tcctggtatg gcttgtacca cagtcgccaa acagacttac
    14641 gccctgcctg cacctcccga tttaagcggt ggcgcgggaa caagttcagt gtctggcccg
    14701 cagggtgaca ttcttccggt cagtaattcg acgctaaaaa gcgaagatcc gaccggcgcg
    14761 ccggtaacca gtagcggttt cctcggcgca ccaacgacct tagcgcctgg tgtactggaa
    14821 ggcagcgaac cgacgcctgc gccacagccc gttgttacag ctccgtcgac aacgcctgca
    14881 acctcgcctg caatggtgac accgcaagcc gcctcgcaaa gcgccagcgg caactttatg
    14941 gtgcaagtcg gggccgttag cgatcaggct cgtgcgcaac agtaccaaca gcaactggga
    15001 cagaagttcg gcgtccccgg tcgcgtaact caaaatggcg cggtctggcg gatccagctt
    15061 ggccccttcg ccagcaaagc cgaagccagt accttgcagc aacgtttgca aaccgaagcc
    15121 caattacagt catttattac caccgcgcag tagcgtaaag caggcatctg atgtgtcaat
    15181 ttctgtaaat gacgttaaca aagtcacgcg gaaagtcaga tgcctgccgg tagtgcattt
    15241 gctatagtag ggcacttttt ttaattccat cacggatgtc gtagttcaga ccatgaatac
    15301 cattttttcc gctcgtatca tgaagcgcct ggcgctcacc acggctcttt gcacagcctt
    15361 tatctctgct gcacatgccg atgacctgaa tatcaaaact atgatcccgg gtgtaccgca
    15421 gatcgatgcg gagtcctaca tcctgattga ctataactcc ggcaaagtgc tcgccgaaca
    15481 gaacgcagat gtccgccgcg atcctgccag cctgaccaaa atgatgacca gttacgttat
    15541 cggccaagca atgaaagccg gtaaatttaa agaaactgat ttagtcacta tcggcaacga
    15601 cgcatgggcc accggtaacc cggtgtttaa aggttcttcg ctgatgttcc tcaaaccggg
    15661 catgcaggtt ccggtttctc agctgatccg cggtattaac ctgcaatcgg gtaacgatgc
    15721 ttgtgtcgcc atggctgatt ttgccgctgg tagccaggac gcttttgttg gcttgatgaa
    15781 cagctacgtt aacgccctgg gcctgaaaaa cacccacttc cagacggtac atggtctgga
    15841 tgctgatggt cagtacagct ccgcgcgcga tatggcgctg atcggccagg cgttgatccg
    15901 tgacgtaccg aatgaatact cgatctataa agaaaaagaa tttacgttta acggtattcg
    15961 ccagctgaac cgtaacggcc tgttatggga taacagcctg aatgtcgacg gcatcaaaac
    16021 cggacacact gacaaagcag gttacaacct tgttgcttct gcgactgaag gccagatgcg
    16081 cttgatctct gcggtgatgg gcggacgtac ttttaaaggc cgtgaagccg aaagtaaaaa
    16141 actgctgacc tggggcttcc gtttcttcga aaccgttaac ccactgaaag taggtaaaga
    16201 gttcgcctct gaaccggttt ggtttggtga ttctgatcgc gcttcgttag gggttgataa
    16261 agacatgtac ctgaccattc cgcgtggtcg catgaaagat ctgaaagcca gctatgtgct
    16321 gaacagcagt gaattgcatg cgccgctgca aaagaatcag gtcgtcggaa ctatcaactt
    16381 ccagcttgat ggcaaaacga tcgagcaacg cccgctggtt gtgttgcaag aaatcccgga
    16441 aggtaacttc ttcggcaaaa tcattgatta cattaaatta atgttccatc actggtttgg
    16501 ttaaaaatta aacacttgaa agtgtaattt ccgtccccat atactaagca tcagtaaaaa
    16561 aactcccgcc ttctggcggg agttgctatt taattacgtt acgccggagc tgacatgaaa
    16621 accaaactta acgaactgct tgaattccct actcctttta cttacaaagt tatggggcag
    16681 gcgttacctg agctggttga tcaggtggtt gaagtggtac agcgccatgc gccaggtgac
    16741 tacaccccaa cggtaaaacc aagcagcaaa ggcaactacc actcggtatc tatcactatc
    16801 aacgccactc atatcgagca ggttgaaata ctgtatgaag aactgggcaa aatcgatatt
    16861 gtccgcatgg ttctgtaact cgcttctccg ttacccggct cctggtcggg taactcccct
    16921 ctcctcgctg tgatatactt ttcccccact tttactcatt ctccacggag atgccgtttt
    16981 gtatcaggat aaaattcttg tccgccagct cggtcttcag ccttacgagc caatctccca
    17041 ggctatgcat gaattcaccg atacccgcga tgatagtacc cttgatgaaa tctggctggt
    17101 cgagcactat ccggtattca cccaaggtca ggcaggaaaa gcggagcaca ttttaatgcc
    17161 gggtgatatt ccggtgatcc agagcgatcg cggtgggcag gtgacttatc acgggccggg
    17221 acaacaggtg atgtatgtgt tgcttaacct gaaacgccgt aaactcggtg tgcgtgaact
    17281 ggtgaccttg cttgagcaaa cagtggtgaa taccctggct gaactgggta tagaagcgca
    17341 tcctcgggct gacgcgcctg gtgtctatgt cggggaaaag aaaatttgct cactgggttt
    17401 acgaattcga cgcggttgtt cattccacgg tctggcatta aacgtcaata tggatctttc
    17461 accattttta cgtattaatc cttgtgggta tgccggaatg gaaatggcta aaatatcaca
    17521 atggaaaccc gaagcgacga ctaataatat tgctccacgt ttactggaaa atattttagc
    17581 gctactaaac aatccggact tcgaatatat taccgcttaa ttccatacat caatggccca
    17641 atttacactg ggtcattact cataatacct gctaatgctt tacatctcct tcgcgtttgt
    17701 atattttctt tatcacttgc agaaatgcgg tgccacgcct tgctccgtaa ctcaccccat
    17761 tgattaacct attgaaatag ttgattatgg tgagcaaaag cgttccgttc caattagaca
    17821 taagattatt gtaatttttt aaatattatt tttccatgaa taaaattgga gtaagtgcgt
    17881 ggatagtaat aatcaaattg aaccctgttt aagcagaaag tcgtcagaag gtaaaccaca
    17941 aatatttaca accttacgaa atattgctct taaccttctg actatttttg aagctgtata
    18001 tgtacataaa gggatcgtta atgcagcgaa agtgcttaat ctgcccccct cggcaatcag
    18061 tcagtctatt cagaaactgc gcgttatatt ccctgaccca ttgtttattc gcaaaggcca
    18121 aggtgtcact cctaccgcat ttgcgatgca tctacatgag tatatcagtc agggccttga
    18181 gtccattctt ggcgcgctgg atatcgaagg aagctatgat aagcaacgaa cgataactat
    18241 tgcgaccact ccctcggtcg gagccctggt ccttcctgtc atctatcggg cgattaaaac
    18301 tcactatccg cagcttttac tgcgcaaccc gcccatcagc gacgcggaaa accaactcag
    18361 tcagtttcaa accgatctca tcatcgataa catgttttgc accaatcgta cggtgcaaca
    18421 tcatgttctg ttcaccgaca atatggtgtt aatttgccgc gagggaaatc cactactctc
    18481 tttagaagat gacagagaga ctatcgacaa cgctgcgcat gtacttctgt taccggaaga
    18541 gcaaaatttc agcggtctgc ggcagagagt tcaagaaatg tttccggacc ggcaaatcaa
    18601 tttcaccagc tacaacattt tgacaatcgc tgcactggtt gccaacagtg acatgttagc
    18661 gattattccc agccgttttt ataacctgtt tagccgctgc tggccgctgg aaaaactgcc
    18721 ttttccgtcc ttaaataagg agcaaataga tttctctatc cactacaaca aattcagcct
    18781 gcgtgatccc atcctgcacg gagtaataga tgttatccgt aatgcatttt gaaaaagtat
    18841 tgttacagcc agatcacagc gcagaacgat aaaatggcac aatccaacaa caattttaca
    18901 ttttcgcgac cgctttggct gctttcaggt ccgtttcaat gatatactgc cagtcgttaa
    18961 ttcaaaaata gttgataatt acaacaatct attgaattga aacgctttcc ttcgtaattc
    19021 gcaactggaa cacgcacgct atgagtaaac ccattgtgat ggaacgcggt gttaaatacc
    19081 gcgatgccga taagatggcc cttatcccgg ttaaaaacgt ggcaatagag cgcgaagccc
    19141 tgctgcgcaa gccggaatgg atgaaaatca agcttccagc ggactctaca cgtatccagg
    19201 gcatcaaagc cgcaatgcgc aaaaatggcc tgcattctgt ctgcgaggaa gcctcctgcc
    19261 ctaacctggc ggaatgcttc aaccacggca cagcaacgtt tatgatcctc ggcgctattt
    19321 gtacccgccg ttgtccgttc tgtgatgttg cccacggtcg cccggtagct cctgatgcca
    19381 atgaaccagt gaaactggcg cagaccattg ccgatatggc gctgcgttat gtggttatca
    19441 cctccgttga ccgtgatgac ctgcgcgatg gcggtgccca gcactttgcg gattgcatta
    19501 ctgccattcg ggaaaaaagc ccgcaaatca aaattgaaac tctggtgccg gatttccgtg
    19561 gtcgtatgga tcgtgctctg gatattctga ctgcaacgcc accagatgtg ttcaaccata
    19621 acctggaaaa cgtaccgcgt atttaccgtc aggtacggcc tggggcagat tacaactggt
    19681 cgctgaagct gctggaacgc tttaaagaag cgcatccgga aatcccgacc aagtctggtc
    19741 taatggtggg actgggtgaa accaatgaag aaattattga ggtaatgcgc gacctgcgcc
    19801 gtcatggtgt gacgatgtta acgctggggc aatatttgca gccaagccgc catcacctgc
    19861 cggttcaacg ttacgttagc ccggatgagt tcgacgaaat gaaagccgaa gcgctggcga
    19921 tgggctttac ccatgctgca tgcggtccgt ttgtccgttc ttcttaccac gccgatttgc
    19981 aggcgaaagg gatggaagtt aagtaatcct gtaataatta cttacatctg tctgataaaa
    20041 aacccgcttt ttgaagcggt ttttttgtat cgaacaagat attgagggag cgtcctgctc
    20101 gccacgtcac tctttatgag agagcttttc agcctgaaga tcaacgtctg cgcctttttt
    20161 cgccgcagca tcatcatcat tcatcgcctt cttgaaccct ttaatggccg ctccaaggtc
    20221 tccgcccagc gtacgtaact tcttagtccc aaacagcaga acgaccagcg ccgcaactac
    20281 tagcagtttg gtaatactaa tctcacccat agataccttc ttgacatata accggcaaca
    20341 gaacgcgcct taaaaaccag agaatattaa cggtcattcg acgcgcgcac acaatagcaa
    20401 tctgtaacaa ggtgaatcaa gcgccgagta aaaaaacatc ataataattg cggcggcgca
    20461 aagcgacggt tgtttaagac gggcagttgc gcgcgcacct gacgcacacg ttcgggcgtc
    20521 acttccgcca taatgagtgc aggcatttct gacgctgccg caatggtgac gccaaaggga
    20581 tctataatcc ggctttgccc gatatttttg ttcccgcact cgcccgccgc caccatataa
    20641 caggtggtat ccagcgcacg agcggcaagc aacgttgacc agtgatgctc tttgagcggc
    20701 ccgcgaaccc aggcggcagg aagtaccagg atttcagctc cctgtaatgc ctgtgccagc
    20761 gccagctctg gaaagcgtaa gtcataacag gtcatcagac cgaccttcat cccttccacc
    20821 tccagtaacg gagcgatttc attaccagca tcaacacggc gtgattcctg aatggcaaat
    20881 gcatcataga gatgcagttt ggcataacgg gcgacgatgt ttcctgcctg aagtgccacc
    20941 agcatattcc atgcgcgccc cggcgttgaa ggaacatgaa tcgtcagaat tgtcgtcatc
    21001 atgttacgtt tactttctcg ccgtaaacgt ccgaggaatt cgccttccag cagctgtgct
    21061 gatttaaccg atagatctgc atcatgatca tcgcgtgcca gcaatgcttc cggcaggaca
    21121 aacagcgata cgtcgttttc cgccgcctgc gccatcaacg aggcacaaat ctcagcgttc
    21181 ttttcccaca cagatgtaac agcaaactgt cctgcagcaa ccagcattgc cacctcctgt
    21241 cagagtcgta aaattcgcca acgacgatta ttcggcgtta cacttatcac tcatacaaat
    21301 caaatagcag gattttgcag tgttacaact tcttttagca gtttttattg gcggtggtac
    21361 gggaagcgtg gcgagatggc tgttaagtat gcgatttaac ccactgcatc aggcgattcc
    21421 gttggggacg ctgacagcaa acctgattgg ggcattcatc ataggaatgg gattcgcatg
    21481 gttcagcagg atgacgaaca ttgatccagt gtggaaagta ttaatcacca ccggattttg
    21541 tggcggtcta acaaccttct caacattttc ggcagaagtg gtgtttttgt tacaagaggg
    21601 tcgctttggc tgggcattac tgaacgtttt cgtcaacctt ctggggtctt ttgccatgac
    21661 agcactggct ttctggctgt tttcggcctc aaccgcacac taaagaccca aaaaaaaccc
    21721 gctgattaag cgggttttga attcttgctg acgtatctta cagagcgatt acgtttgcag
    21781 cagaagggcc tttggcaccg ttagtgattt cgaactctac gcgctgacct tcagcaagag
    21841 ttttaaaacc attagtctgg attgcagaga agtgtacgaa cacgtctttg ctgccgtctt
    21901 ccggagtaat gaaaccgaat cctttggact cattaaacca cttaacgtta cctttaatct
    21961 tagacatcaa aattaccttt acatgaaaaa tagacacaaa tgctgcgtcg gttaccagta
    22021 caccaattgt ggtacgcttt gtccagtgca actttactaa aaagtgataa aagtcgcgtt
    22081 atttttgatg ttacttattt tagctattga ttttaaagaa gttactaaaa cttcatttgt
    22141 ctcaaaactg aaagcgcatc caggcaaagt acacattgcc attgttgtag gtacccggaa
    22201 tgtaggtcat ctgaaaagtc actgggccat aacccacgga ggccaatggc agtagaaccg
    22261 ggagagggat gtaattccag ttatcgcgtg ccgttacgcc agcggtgaat cccagaccta
    22321 aatgaaaatt ttcatccgcc agcggtcgcc aggtactttc ccatccgtat ccggcaatcg
    22381 gttcccattt gttccacgag tccttaaatg ccatggcata caggccatgc cagtttcctt
    22441 tttcatccca acgcgacagg ccaaaaccgc caccccacgg tcgctcgtta tagcgatcgg
    22501 ttttttcttt gtcgtaagcg aaacgtgcat gccaggtgat ggcaggaata tataaatcat
    22561 aatgttcagg ctgttgccag gttggtgcaa tattttctct aaacgttgtc atccactcat
    22621 ctgcgttagc aaaaacttta ccaacgctga ttaactgaat aaaaacaaag gaaaagatag
    22681 cgacatattt actcacgttc atttgtgacc ataaaacatt tatcaaaaat ctactactag
    22741 catagcaaag ctacaattaa cataacctta atagacccaa cataaagaat aatctgaata
    22801 gctgctctat ttatttccgc ttgttcaagt taaatatttc cgcgcatcga ttaaagatga
    22861 tgcgtcgttt tctgcgatgg gaatagtcaa aaaagaaaaa ccaagtcttt tttgatgaca
    22921 aaatgcaatc aaggaaaaat tattttattt tttaacgaga tacaacaatc accttaacaa
    22981 agtatataat atatatcgtc aacctatgct catattagat tattttttga gcgcacagcc
    23041 gcagctaaca ccattgcaat taacaaattt gcatcaatcc accatcaatt tgcacacatt
    23101 attatgtgat aattgccaac cgctaaatat gcgttttgtt atctatgtat aaaaacagta
    23161 acttcaatgt cttaatggca gtttttcttg attttaatca gcattcatcg ccaatttatt
    23221 gggcatattt tttccttaag ctttaggaat tttttattat ttactttggg gcctggagac
    23281 aggaaaaatt atgctgacat tcattgaact ccttattggg gttgtggtta ttgtgggtgt
    23341 agctcgctac atcattaaag ggtattccgc cactggtgtg ttatttgtcg gtggcctgtt
    23401 attgctgatt atcagtgcca ttatggggca caaagtgtta ccgtccagcc aggcttcaac
    23461 aggctacagc gccacggata tcgttgaata cgttaaaata ttactaatga gccgcggcgg
    23521 cgacctcggc atgatgatta tgatgctgtg tggatttgcc gcttacatga cccatatcgg
    23581 cgcgaatgat atggtggtca agctggcgtc aaaaccattg cagtatatta actcccctta
    23641 cctgctgatg attgccgcct attttgtcgc ctgtctgatg tctctggccg tctcttccgc
    23701 aaccggtctg ggtgttttgc tgatggcaac cctatttccg gtgatggtaa acgttggtat
    23761 cagtcgtggc gcagctgctg ccatttgtgc ctccccggcg gcgattattc tcgcaccgac
    23821 ttcaggggat gtggtgctgg cggcgcaagc ttccgaaatg tcgctgattg acttcgcctt
    23881 caaaacgacg ctgcctatct caattgctgc aattatcggc atggcgatcg cccacttctt
    23941 ctggcaacgt tatctggata aaaaagagca catctctcat gaaatgttag atgtcagtga
    24001 aatcaccacc actgctcctg cgttttatgc cattttgccg ttcacgccga tcatcggtgt
    24061 actgattttt gacggtaaat ggggtccgca attacacatc atcactattc tggtgatttg
    24121 tatgctgatt gcctccattc tggagttcct ccgcagcttt aatacccaga aagttttctc
    24181 tggtctggaa gtggcttatc gcgggatggc agatgcgttt gctaacgtgg tgatgctgct
    24241 ggttgccgct ggggtattcg ctcaggggct tagcaccatc ggctttattc aaagtctgat
    24301 ttctatcgct acctcgtttg gttcggcgag tatcatcctg atgctggtat tggtgattct
    24361 gacaatgctg gcggcagtca cgaccggttc aggcaatgcg ccgttttatg cgtttgttga
    24421 gatgatcccg aaactagcgc actcttccgg cattaacccg gcgtatttga ctatcccgat
    24481 gctgcaggcg tcaaaccttg gccgtaccct ttcgcccgtt tctggcgtag tcgttgcggt
    24541 tgccgggatg gcgaagatct cgccgtttga agtcgtaaaa cgcacctcgg taccggtgct
    24601 tgttggtttg gtgattgtta tcgttgctac agagctgatg gtgccaggaa cagcagcagc
    24661 ggtcacaggc aagtaaatag taatgccgga ggcaagtttc ctccggcttt atcatgtcac
    24721 ccactgtggt atatgcgttg tggtctgcca actttgccgt gaacaatttc agcaataatc
    24781 agatggcggc tggcgcaata ttcaagataa cgtctggcag acgtgcggct gatggtcagt
    24841 gcctgcgcca ccgtttctgc cgtatgttgc acaccaggct ctttaaacag ttttcgcacc
    24901 gcgtttagcg tcaagggatc aatgccggtc ggcagttcgt ctttaggttc accgcgagca
    24961 taggcattaa acatctcatc aatttgcttc tggctggcgc tatcaatact ttccagcata
    25021 tgtttacgct ggcggaaacg ggttagcgtt tgccccagcc gttcataggc aatgggctta
    25081 atgagataat caaatacacc acaacgtacg gcttcagaca ccgtttccat atcgctggct
    25141 gcagtggtaa acaccacgtc gccgggataa tgcgcctgca ccagttcatg caataaatta
    25201 atccctctgc cgtcaggaag atagttatcg agcaagatta gccccggctt aaaacgctcg
    25261 atcatcattc gggcctgcgc caggtttccc gccagtaata tctgactgaa tccgggaatg
    25321 tgacgaatat attccgcatg catctctgcc agcggcgttt cgtcctcaac gatcaatagg
    25381 gttaatggag ctgtcattag gtttcacttt cggaatatag attgaaaata aggtaccgca
    25441 gggatcatta tcttcgagag tgataacacc accgcagcgc gttacgtagc tggcaatcaa
    25501 gtacaaccca atgccatgtt caccgggctc gtcagcacgc gtactgaccc cctgctcaaa
    25561 tattttgtct cgtagagact ctggaacgcc gcagccctga tcggcgactt caatcaccac
    25621 atcatcgcct tcatcgctga ggaataattc aacgatcttg tttccttcat cgctacgcag
    25681 gctggcttcg aaggcgttat caagtaaatt gcccacaatg gctgcaaact cggtgctatc
    25741 cagtcctggc ggcagttgcg aaagctggct accggggaca atgatcattt ttagccccag
    25801 ttcccgggcg cgctgcactt taccaaaaag cagccccgcc acctagcgat cggcaaacgc
    25861 ctcgcgcagg ctgtcaataa gctgttgctg ggcctgagac tccccctgca ccatcgccag
    25921 cacgcgatca tactctttca tctgcaacag accattgagc gtcgacatcc aattgagatg
    25981 ctcgtgtcgc aatgtacgaa ggctctcaac gtattgtttt atttgcgtca gttgcgcatt
    26041 gagggtggaa atttcgtctt tactgcgaaa gctgataatg gcccccagca aatcatcacc
    26101 tgaacgaata gcttcccgat tggcaataac gctcagaccg ttaaagttag ccaccatatc
    26161 ctgacgtttt tcatcaatct gttcggtaaa gaaatcggcg ggcctgacca cttcagcaat
    26221 gggtttaccc aaccattgcc gtccggggga actcagcccc agcatctttc ttgcgttacg
    26281 attgatggcg gtaatgtaac cgtgcggatc caccgcaatc agcccttcat aaaccgaact
    26341 aaacagcgcc tcttgctgac ggaccacgcg tgcgatttgc tttggctcca tgcccatcat
    26401 ctgccgacgg atatgcgcgg ccaggaacca cgacagcaac atcagaatcc ctaacagcac
    26461 gacaaacaca cctgccatcg gtaataaaaa ctcagcccgc cagctatcga ttttactcac
    26521 caggtagcca atcgacacca cgccgatgac ttttccatcg tcatcaaaga ttggcgtttt
    26581 ggcgcgcatc gccatcccca ttgacccttt cccggtaatg aagtagctct cccctttctc
    26641 cagcgcgccc tgtttggtga actgcatagg ataaccaatt ttctccggat taggatggta
    26701 aaggcggatc gagtgccggt cgccatcacc acataatcaa aatcggtatc tctttgtaat
    26761 ttgttagcga tggtcgccag ccgtttgtag tcacgcgttt tcaccgcaga gatgacactg
    26821 tcattggagg caataatttt cgcctgattc attgccatgt cgcgtacatg aagcgttaaa
    26881 tagtcctcaa aactggccgt aaaatattgc gccaaggctg caatgacaaa tattgagaac
    26941 accagaatca gcaaaaagat acgcagcgga aatgccagtc tttggaaaaa tgcaaactgt
    27001 ttattctcgt taagctgcaa cattgttttc cttgcgggtt atcccgagcc tctttgacag
    27061 atgtaattaa aatgttgaat caaaatacat agatattaat tatgatcgtg gcaaatttta
    27121 ttcattaaat caattaaatc acttaaacct gcaggtcatt tcgtaattac attaattaaa
    27181 aaacttaaac cattaaataa ataaaaattt cacgccgaaa tactccttag gatgtatagc
    27241 gaaaggagaa aaagatatac ctcgatcacc cccctttctc ccaagtgaaa ataaaaggtt
    27301 atcagtttgc aacattgaac aacattcgtt gcaaatcgat aacaacatgc accttcagga
    27361 tactatttat tatgttcggc aatgatattt tcacccgcgt aaaacgttca gaaaataaaa
    27421 aaatggcgga aatcgcccaa ttcctgcatg aaaatgattt gagcgttgac accacagtcg
    27481 aagtatttat taccgtaacc cgcgatgaaa agcttatcgc gtgcggtgga attgccggaa
    27541 atattattaa atgcgttgct atcagtgaat ccgttcgcgg tgaaggactg gcgctgacat
    27601 tagccactga actgataaac ctcgcctatg agcggcacag cacgcatctg tttatttata
    27661 ccaaaaccga atacgaggcg ctgttccgcc agtgcggttt ttccacgctg accagcgtac
    27721 ccggcgtgat ggtgctgatg gaaaacagcg ccacgcgact gaaacgctat gccgaatcgc
    27781 tgaaaaaatt tcgtcatcca gggaacaaga ttggctgcat tgtgatgaac gccaatccct
    27841 ttacgaatgg tcaccgttat ctgattcaac aagctgcggc acagtgcgac tggttgcatc
    27901 tgtttttagt caaagaagat tcttcacgct tcccctatga agaccggctg gatctggtgt
    27961 taaaaggcac cgccgatatt ccacgcctga ctgtgcatcg cggctccgaa tacatcatct
    28021 cccgcgctac gttcccttgc tacttcatta aagaacagag cgtcattaac cattgttaca
    28081 ccgaaattga tctgaagatt ttccgtcagt acctcgctcc cgcactgggt gtaactcacc
    28141 gctttgtcgg tactgaaccc ttttgtcgcg ttaccgccca gtacaaccag gatatgcgct
    28201 actggctgaa aacgccgact atctccgcac cgcccatcga actggttgaa attgagcggc
    28261 tgcgttacca ggagatgccg atatccgctt cccgggtacg tcaactgctg gcgaaaaacg
    28321 atctcacggc tatcgcgccg ctggtccctg cagtcacgct gcattatttg cagaacctgc
    28381 ttgagcactc ccgccaggac gcggcagctc gtcaaaagac ccccgcatga gaaacaggtg
    28441 aaaaatgaaa ataaaccagc ccgccgttgc aggcaccctt gagtctgggg atgtgatgat
    28501 acgcatcgcc ccactcgata cgcaggatat cgacctgcaa atcaacagca gcgttgagaa
    28561 acagtttggc gatgcaattc gcaccaccat tctggacgtt ctcgcccgct acaacgtgcg
    28621 cggcgtacag ctgaatgtcg atgacaaagg cacactggac tgcattttac gtgcacgact
    28681 ggaagccctg ctggcacgcg ccagcggtat cccgactctg ccatgggagg attgccaatg
    28741 atttccgctt cgctgcaaca acgtaaaact cgcacccgcc gcagcatgtt gtttgtgcct
    28801 ggtgccaatg ccgcgatggt cagcaactcc ttcatctacc cggctgatgc cctgatgttt
    28861 gacctcgaag actccgtggc attacgtgaa aaagacaccg cccgccgcat ggtttatcac
    28921 gcgctgcaac atccgctgta tcgcgatatt gaaaccattg tgcgtgtcaa cgcgctggat
    28981 tccgaatggg gtgttaacga cctggaagcc gtcgttcgcg gtggtgcgga cgttgtgcgt
    29041 ctgccgaaaa ccgataccgc tcaggatgtt ctggatattg aaaaagagat cctgcgtatc
    29101 gaaaaagcct gtggtcgtga acccggcagc accggcctgc tggcggcgat tgaatctccg
    29161 ctgggcatta cccgcgcagt ggaaatcgct cacgcttccg agcgtttgat cggtatcgcc
    29221 ctcggtgcag aagactatgt gcgcaacctg cgtacagaac gctccccgga aggaactgaa
    29281 ctgctgttcg cacgctgttc cattttgcag gccgcgcgct ctgcgggtat tcaggcgttc
    29341 gataccgtct attccgacgc taacaacgaa gccggattcc tgcaagaagc cgcccacatc
    29401 aaacagctgg gctttgacgg caaatcgctg atcaacccgc gtcagattga tctgctgcac
    29461 aacctctacg caccgaccca gaaagaagtg gatcacgccc gccgcgtcgt agaagccgct
    29521 gaagccgccg ctcgcgaagg cctcggcgtg gtttccctga acggcaagat ggtggacggt
    29581 ccggttatcg atcgcgcccg tctggtgctc tcccgtgcag aactttccgg catccgcgaa
    29641 gaataaggca atcaaaatga cgcagaaaat tgaacaatct caacgacaag aacgggtagc
    29701 ggcctggaat cgtcgcgctg aatgcgatct tgccgctttc cagaactcac caaagcaaac
    29761 ctaccaggct gaaaaagcgc gcgatcgcaa actgtgtgcc aacctggaag aagcgattca
    29821 tcgctctggt ttacaggacg gcatgacggt ttccttccat cacgctttcc gtggcggtga
    29881 cctgaccgtc aatatggtga tggacgtcat cgcgaagatg ggctttaaaa acctgactct
    29941 ggcctccagc tccctgagtg attgccatgc gccgctggta gaacacattc gccagggcgt
    30001 ggttacccgc atttatacct ctggcctgcg cggtccactg gcggaagaaa tctcccgtgg
    30061 tctgctggcg gaaccggtgc aaatccactc tcacggcgga cgtgtgcatc tggtacagag
    30121 cggcgaactg aatatcgacg tggctttcct cggcgtccca tcctgtgatg aattcggtaa
    30181 tgccaacggt tacaccggta aagcctgctg tggctccctc ggctatgcaa tggttgatgc
    30241 cgacaacgca aaacaggtcg tgatgcttac cgaagaactg ctgccttatc cgcataatcc
    30301 ggcaagcatt gagcaagagc aggttgattt gatcgtcaaa gttgatcgcg ttggcgatgc
    30361 tgcaaaaatc ggcgctggtg cgacccgtat gaccactaac ccgcgcgaac tgcttattgc
    30421 ccgtagcgct gcggatgtga ttgtcaactc tggctacttc aaagaaggtt tctccatgca
    30481 aaccggcacc ggcggcgctt cgctggcggt aacccgtttc ctggaagaca aaatgcgtag
    30541 ccgcgatatt cgcgccgact tcgccctcgg cggtattact gcaacgatgg ttgatctgca
    30601 cgaaaaaggt ctgatccgca aactgctgga tgtgcagagc tttgacagcc atgctgcgca
    30661 atcgctggcc cgtaacccca atcacatcga aatcagcgcc aaccagtacg ctaactgggg
    30721 ttcgaaaggc gcatcggttg atcgtctcga cgtggtggta ctgagcgcgc tggaaattga
    30781 cacccagttc aacgttaacg tgctgaccgg ctctgacggc gtactgcgtg gtgcttccgg
    30841 tggtcactgc gataccgcga ttgcctctgc gctttccatc atcgtcgcgc cgctggtacg
    30901 cggtcgtatt ccgactctgg tggataacgt gctgacctgc atcaccccgg gctccagtgt
    30961 cgatattctg gtcacagacc acggtatcgc agttaacccg gcacgtccgg agctggcaga
    31021 acgtctgcag gaagcgggca ttaaagtggt ttccattgag tggctgcgtg aacgtgcgcg
    31081 tctgctgacc ggtgaaccac agccgattga attcaccgac cgcgtcgttg ccgttgtgcg
    31141 ttaccgcgat ggctcggtga tcgatgttgt acatcaggtg aaggaataag ccatgcacct
    31201 gcttcctgaa ctcgccagcc accatgcggt gtcaattccc gagctgctcg tcagccggga
    31261 tgaaaggcaa gcacggcaac acgtctggct caagcgccat cctgttccac tggtctcctt
    31321 taccgtggtt gcgcctaggc cgattaaaga cagtgaggtc acacgccgaa tttttaatca
    31381 tggcgtgaca gccttgcgtg ccttagccgc aaaacagggc tggcaaattc aggagcaggc
    31441 tgcactggtt tccgccagcg ggccggaggg tatgttgagc attgccgccc ctgctcgcga
    31501 cctcaagctc gccaccattg agcttgaaca tagtcatcct ctcgggcggt tatgggatat
    31561 cgatgtcctg acgcccgaag gcgaaattct ctcccgccgc gactattcac tgccgcctcg
    31621 ccgctgcctg ttgtgcgagc aaagcgcagc cgtctgcgcg cgtggaaaac ccatcaactg
    31681 accgatttac tcaaccgcat ggaggcactg ctgaacgatg tcgatgcctg caacgtcaac
    31741 taaaaccaca aagcttgcga cgtcattaat cgatgagtac gccctgctgg gctggcgcgc
    31801 catgctgact gaagtcaatc tgtcaccgaa accaggtctc gtggatcgca ttaactgcgg
    31861 tgcgcataaa gatatggcgc tggaagattt ccaccgcagc gcgctggcga ttcagggctg
    31921 gctaccccgt ttcattgaat ttggtgcctg tagtgcggaa atggcaccag aagcggcact
    31981 ccacggatta cgcccaattg gtatggcttg caaaggtgat atgttccgcg ccaccgcggg
    32041 cgtaaacacg cataagggca gcattttttc tttagggcta ctgtgtgcgg caattggccg
    32101 tttgcttcag ctcaaccagt cgctaacgcc aacaaccgtt tgttctacgg cggcaagttt
    32161 ctgccgtggc ctgaccgatc gcgaactgcg taccaataat tcacaactga cggcaggtca
    32221 acggttgtac cagcagcttg cccttaccgg cgcacgcggt gaagccgaag cgggttatcc
    32281 actggtgatc aaccacgcct tgccgcatta cctcactctg ctggatcagg ggttagatcc
    32341 tgaactggca ttgctcgata ccttgctcct actgatggcg atcaacggcg ataccaacgt
    32401 tgcttcgcgc ggtggcgagg gggcctgcgc tggctacagc gcgaggcgca aacattattg
    32461 caaaaagggg gcattcgaac ccccgccgat ctcgattatc tccggcagtt cgacagggag
    32521 tgtatcgaac gaaatctcag tccaggcggc agtgccgacc tgctgatcct tacctggttt
    32581 ttagcacaga tttaattatt taagcacttg ataaatttgg aaatattaat tttcggagaa
    32641 cccgtatgtc tttagcaaaa gataatatat ggaaactatt ggccccactg gtggtgatgg
    32701 gtgtcatgtt tcttatccct gtccccaacg gtatgccacc acaggcatgg cattacttcg
    32761 ctgtgtttgt ggcaatgatt gtcggcatga tcctcgagcc aattccggca acagcgatca
    32821 gttttattgc ggttactatt tgcgttattg gcagtaatta cctgctcttt gatgccaaag
    32881 aattagctga cccagcgttt aatgcgcaaa aacaggcgct gaaatggggt ctggctggtt
    32941 tttccagcac cacggtatgg ctggtatttg gcgcatttat ttttgcatta gggtatgaag
    33001 tttccgggtt aggtcgtcgc attgcccttt tcctggtgaa attcatgggc aaacgcacgc
    33061 tgacgttggg ttatgcgatt gtcattatcg acattctgct ggcgccgttt acaccgtcca
    33121 acaccgcgcg taccgggggt acggtttttc cggtcattaa aaacctgccg ccgttgttta
    33181 aatcattccc caacgatccg tccgcgcgtc gtattggcgg ctatttgatg tggatgatgg
    33241 tcattagtac cagtctgagt tcgtccatgt ttgtcaccgg tgcggcacca aacgtgctgg
    33301 gtctggagtt cgtcagcaaa attgccggta tccagattag ctggttgcag tggttcctct
    33361 gcttcctgcc ggttggggtt atcttgctta tcattgcgcc gtggctttcc tacgtgctgt
    33421 acaaaccgga aatcacacac agtgaagaag tggcaacctg ggcgggtgat gaactgaaaa
    33481 ccatgggtgc gctgacacgc agagagtgga cactgattgg ccttgtattg ctcagcttag
    33541 gtttgtgggt ttttggcagt gaagtcatta atgctactgc agttggtctg ctggcagttt
    33601 cgctaatgct ggccctgcac gttgtgcctt ggaaagatat tacccgctat aacagcgcat
    33661 ggaacacgct ggtcaacctg gcaactctgg ttgtgatggc taacggcctg actcgttctg
    33721 gctttattga ctggttcgcc ggtaccatga gtacgcacct ggaaggattc tcaccaaacg
    33781 caacggtgat tgtactggtt ctggtgttct actttgcaca ctacctgttt gccagcctgt
    33841 ctgcgcacac cgcaaccatg ctgccggtta ttctggccgt cggtaaaggt attccgggcg
    33901 taccaatgga acaactgtgt atcctgctgg tgctgtctat cggtatcatg ggctgtctga
    33961 cgccctatgc aaccggtcct ggggtgatta tttacggctg tggctatgtg aaatcaaaag
    34021 attactggcg tcttggcgca atcttcgggg tgatttatat ctctatgttg ctgttggttg
    34081 gctggccgat tctcgccatg tggaactaat acgttcgcca aacgctgaat aaatcatcac
    34141 gcccgccagg taagcccacc tggcgggcgt gatgatttaa tagatagtat tcagtgtatg
    34201 tatgagttcc acacccatta tgaaagaatt ctggcgtaac gccgcgttgc tcgcggtttc
    34261 tctgcttccc ttctcttctg ccaacgccgt agcgttgcag gcaaaacagt atggcgattt
    34321 tgatcgctat gtcctggccc tctcctggca aaccggattt tgccagagtc aacacgatcg
    34381 aaatcgtaac gaacgagatg aatgtcgcct gcaaaccgaa acgaccaaca aagctgattt
    34441 tctgaccgta catggtctgt ggccaggatt gcctaaatcg gttgctgccc gtggtgttga
    34501 tgaacgccgc tggatgcgct tcggttgcgc tactcgccca atcccgaatc taccagaagc
    34561 gcacgccagc cggatgtgtt catcgccgga aaccgggtta tcactggaaa cggccgctaa
    34621 actaagtgaa gtcatgccag gagctggcgg acgttcctgc ctggaacgct acgaatatgc
    34681 caaacacggt gcctgctttg gttttgatcc ggacgcatac ttcggtacga tggtacgcct
    34741 gaatcaagaa attaaagaga gcgaagccgg aaaattcctt gcggataatt acggtaaaac
    34801 agtgagccgc cgtgactttg acgccgcctt tgccaaaagc aggggaaaag agaacgtgaa
    34861 agcagttaag ctaacgtgcc agggtaaccc tgcgtatttg actgaaattc agatctcgat
    34921 caaagctgac gccatcaacg ctccgctttc tgcaaactca ttcttgccac aaccacaccc
    34981 aagcaactgt ggcaaaacct ttgtgattga taaagtgggt tattaatcat ctgatttaca
    35041 aaacacgcta attcgtcttc cataaaccgt gacgattaag aattatacat aatgcgctgc
    35101 attgaggaat tccctgaatg tgacgcaaat cacttcaaag agtgagcaac agtcagtatc
    35161 atcatgtgag ttaaaccctc gccgcctgac ggtgagggtt ttcttttggg tatgtttatc
    35221 tgcgacactc gcagtaccga cgacatggag taaaaatgtc cagaccaact atcatcatta
    35281 acgacctgga tgccgaacgc atcgatattc tgctggagca acccgcctat gctggtttgc
    35341 caatcgccga cgcgttaaac gcagagttgg atcgcgccca aatgtgttcg ccagaagaga
    35401 tgccacacga cgtggtgaca atgaacagcc gggttaaatt ccgcaatctt agcgatggcg
    35461 aagtgcgtgt gcgcacgctg gtgtatccgg caaaaatgac cgatagcaat actcagcttt
    35521 ccgttatggc tccggtaggt gccgcactgc tggggctgcg cgttggcgat tccattcact
    35581 gggaacttcc gggcggcgtt gcaacccacc ttgaagtgct ggaactcgaa taccagccag
    35641 aagctgctgg cgactacctg ctttaatccc atctgcacaa gcgataactc gctttgccca
    35701 gaggatgcac tcgcatcctc tttacgcaaa atcagcaatt tttcctggtt ctcactgttc
    35761 acttttgctg caaaattatt ccgtttcgtt tgtgggaata accatggcag aagcatttta
    35821 catcctgata ggatttctga ttatggcggc gattatcgtc atggctgtcc tttacctcga
    35881 aaaccactcc tgacgatcat attgttcccc ccggcatcgc attcaccaga cctgaaactg
    35941 ccttctgcgc ctcctctgcg ctttgtgcac cgggtaccag aatcacctta cggcactcct
    36001 cttcacgttt ttcgaaaatc tcatatcccc gggcggcctc ttcaaacggc atatagtggg
    36061 taacaatttc ttctggtttc agtaatcctt tctcaattaa cggtaataat tctcccagcc
    36121 atgcgtgaac gtgggtctgt cccattttaa acgtcaaccc tttatcaaag gcgtcaccaa
    36181 acaggaaacc gtgaataaat ccagcgtaga cgcccggtac gctaacaatg ccgccacgcc
    36241 tgaccgccgc aatacactgg cgcaacgctt taccgctgct gccctccagt ttcaggttag
    36301 tcagcaccgt ttccgtggtg ctgcctttcg cttcaaaacc gacggcgtct attactgcat
    36361 ccacgccccg gtgacctgcc gtttgttcaa taattgactg tgccggatcg ctatcttcat
    36421 cgaaattaat cgggatcgcg ccgtagcggt cagcggcgaa actcaagcgg tagggatgat
    36481 gatcaacaac aaaaatctgt tccgcaccga gcaaccgtgc acaggcgatt gtcaacaatc
    36541 ccactggacc ggcaccatag actgcaacgc ttgaaccttg ttggatctgc gcattttttg
    36601 ctgcctgcca tgccgttggc agaatatcag aaaggaaaag ggctttatca tctgaaagca
    36661 aaggcggtac tttaaacggc cccacattcc ctttagggac gcggacatat tccgcctgtc
    36721 caccaggaac gccaccatac aggtgactat aaccaaacaa tgccgcggga gctggtatct
    36781 gttttttatt gagcgcagcg cctttacccg cattggtatt ttcgcaggcg gcatattgct
    36841 gcattcgaca gaaaaaacag tcgccacaag caatgacaaa cggaattacc acccggtcgc
    36901 ctttttgcaa attttttacg tcctttccgg tttcaacgac ttcccccata aattcatgac
    36961 caaaaatatc gccatgttta acctggggta ttttgcctcg ataaagatgg agatcagagc
    37021 cacagatcgc cgttgccgta atacgcagaa taatatcatc tgcctgttca atgcccggat
    37081 cgggaacatt ttctacctga acgtgatgtg ggccgtgata agtcaatgct ttcatgcaac
    37141 ctccagagac agttcaaaaa agagtcagtc tttaagggta gctatcacag cgggaaaacg
    37201 ggcggtaccg caggttttgg gagaattcct ggtgttcagt gagaatatat taatcacaga
    37261 aaccgttcgt aaggaaaaag aaccagacaa atatcaggga taaatattat aatcatggcg
    37321 aatgatttgt tcatgattaa cagggagaaa gggttatgta taagacaatc attatgccag
    37381 ttgatgtatt tgaaatggaa ttgagcgaca aagctgttcg ccacgctgaa ttcctcaccc
    37441 aggatgacgg agttattcat ctacttcacg tactacccgg gtcagccagc ctgagcctgg
    37501 accgttttgc cgctgatgtg cgtcgttttg aagagcatct gcaacatgaa gcagaagaac
    37561 gtctgcaaac gatggtcagc cacttcacca tcgatccttc ccgcattaaa caacatgtcc
    37621 gttttggtag cgtgcgggat gaagtcaatg agttggcaga agaactgggg gctgatgttg
    37681 tagttatcgg ttctcgcaac ccatcgattt cgacccatct gttaggttct aacgcctcga
    37741 gcgtgatccg ccacgccaat ctgcctgtac tggtcgttcg ctaacagata aaagtattta
    37801 tgccgccctg cctggaacat cccggcaggg ctttttaatg ccataaaaaa gccgctaggt
    37861 ttgaccccgg cggctaagca attgcaggtg aatcttactt cttcttatgc agttttggtg
    37921 cgaatcaggt agtcaaaagc actcagagag gctttggcac cttcgccagt ggcgatgatg
    37981 atctgcttgt acggaaccgt cgtacagtca cccgctgcga atacgccttt gacgttggtt
    38041 tcgcatttcg catcaatgat aatctcaccc atgcggttac gttcgactgc gccttcgagc
    38101 cagttggtgt tcggcagcag accaatctgc acgaaaatac cggccagttc gatgttgtga
    38161 atatcgccgc tgacacgatc tcgatattcc agaccaacga ctttgctgcc gtcgcctttc
    38221 acttccgtgg tttgcgcatt cagaataatg tcgacgtttt tcaggctgcg cagtttgtcc
    38281 tgcagaacct ggtcggcttt catttctggc gcaaattcca gcagcgttac gtgctcaacg
    38341 atacccgcca ggtcaattgc cgcttccacg ccggagttac cgccgccgat aaccgctacg
    38401 cgtttacctt taaacagcgg gccgtcgcag tgcgggcagt aggtcacgcc tttggtgcga
    38461 tactgatctt cgcccggaac gttcatgttg cgccattttg caccggtcgc cacgataatg
    38521 ctgcgtgctt tcagtaccgc gccagaagct gtttcaatct gatgcagacc accttcaact
    38581 gctgctggga tcagtttgct ggcgctctgg ctgtcgatca catcaacgtc gtattcatca
    38641 acgtgaactt tcagtgcgcc tgccagcttc tgcccttcag tcttcggtac agaaatgtag
    38701 ttttcgatat caacggtatc gaggatctga ccaccaaaac gttcgcccat cagaccggta
    38761 cggatgcctt tacgtgcgga gtaaattgct gccgctgcac ccgccgggcc ggaaccgacg
    38821 attaatacgt cataagcatc acgcttgttc agctcttctg ccgcacgttt ttccgcgccc
    38881 gtatcaattt tggcaacgat ttcagtcaac gtcatgcggc cctgaccaaa ctctttcccg
    38941 tttacgaaca ctgccggaac gcccatcacg ttgcgatcgg tgatttcgtt ctggaaggtg
    39001 ccgccgtcaa ttgcagtgtg cttgatgcgc gggttcagta cgctcatcag gttcagcgcc
    39061 tgcaccacgt ccgggcagtt atggcaagag agcgagtaat aggtttcgaa ttcaaaatca
    39121 ccgtcaatat ggcgaatctg ctccagcaga gactgcgctt ctttcgacgg atgaccaccg
    39181 gtccacagca acgccagtac cagcgaggtg aactcgtggc ccagcgggga gcctgcaaaa
    39241 cgtggcccct ggttggaacc taggttggtg atcaggaaag acggcttacg caccggcaag
    39301 ctgttatctt ctttaaaggt gactttgtct gacagttctg cgatttcagc caacagttcc
    39361 ttgatttctg ccgatttagc gctgtcatcc agcgtggcaa ttaactcaac aggcttggtc
    39421 aatttctcaa ggtaagcctt gagttgagtt ttcatatttg tgtcgagcat gtttatctcc
    39481 tgggctttaa acatcatcat gcaagcggcc tctacggcta cctggatgca gcttgcatca
    39541 tagtgcgggg aagaaaatcg ggtgccgcag cacccgaaga attaggtgaa tttccgaagt
    39601 atttcgcgcc ggagttggta gcgcgaaaga cgaaggaaat ttagatttta ccaaccaggt
    39661 ccagagacgg agccagagtt gcttcacctt ctttccattt agccgggcaa acttcacctg
    39721 ggtgagaagc tacgtactgt gctgctttga ttttacgcag caggtcagac gcgtcacggc
    39781 caatgccttc agcggtaact tcgattgcct ggatgatacc ctgcgggtca acaacgaagg
    39841 tcgcacggtc agccagacct tcatcttcac gcatgttgtc gaagttacgg gtcagggcgc
    39901 cagtcgggtc gccgatcatc gcatatttga ttttagcgat ggtttcagag ctgctgtgcc
    39961 atgctttgtg ggtgaagtga gtatcggtag atactgcgta tacgtctacg cccagtttct
    40021 gcagttcttc gtagtggtca gcaacgtcac ccagttcggt cgggcatacg aaagtaaagt
    40081 cagccgggta gaagaagaag acgctccagc ggccttcggt atctttttcg gtgatttcga
    40141 tgaattcgcc gtttttgaat gcctggtttt taaaaggttt aattttggtg ttaatcaagg
    40201 acatctatac ttcctccgtg ttttcgatga gatgtaaggt aaccaatttt tgccgattcg
    40261 gctaatgcgt ttccattatc aaatcgataa gttttacctt acaaccttcg taagacaact
    40321 ttgtgaactt tgcaccgcca gcggcgataa aaagtaaaat ggcccccttt ccaggtgacc
    40381 gtgtaatctg aactaccctt tggtatcttc agcgcctgcc tgcaggctga ggcgttgccc
    40441 ttacaaatgc aacaacgaca tggattacaa caccctcata aacaaagggc aatcacctga
    40501 tctaagctct tacctatgac agtgataggt tatgcctttt actcgacttt tgcactgact
    40561 gaaaaggaca aattaatgtt aaaaaagata cttttactgg ctctgcttcc tgcaatcgcc
    40621 ttcgcagagg aacttcctgc tccagtaaaa gcgattgaaa aacagggcat tacaatcatc
    40681 aaaacgttcg atgcccccgg aggaatgaaa ggttatctcg gaaagtatca ggatatgggc
    40741 gtcaccatct acctgactcc agatggtaag cacgctatct ctggttacat gtacaacgag
    40801 aaaggtgaaa acctgagtaa cacacttatc gaaaaagaaa tttacgcacc agccggacgc
    40861 gaaatgtggc aacggatgga acaatcccac tggctcctcg acggtaaaaa agatgcgccg
    40921 gtcattgtct acgtcttcgc cgatccgttc tgcccatatt gtaaacagtt ctggcagcag
    40981 gc
//
LOCUS       DAJVUJ010000012        40465 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.012, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000012 DAJVUJ010000000
VERSION     DAJVUJ010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40465
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.012"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(392..598)
                     /locus_tag="OQQ48_000658"
     CDS             complement(392..598)
                     /locus_tag="OQQ48_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HCS1951531.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMEQAEKATGDFY"
     gene            911..1000
                     /gene="kdpF"
                     /locus_tag="OQQ48_000659"
     CDS             911..1000
                     /gene="kdpF"
                     /locus_tag="OQQ48_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HCS1951532.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            1000..2673
                     /gene="kdpA"
                     /locus_tag="OQQ48_000660"
     CDS             1000..2673
                     /gene="kdpA"
                     /locus_tag="OQQ48_000660"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HCS1951533.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2696..4744
                     /gene="kdpB"
                     /locus_tag="OQQ48_000661"
     CDS             2696..4744
                     /gene="kdpB"
                     /locus_tag="OQQ48_000661"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HCS1951534.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPADVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADIAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSITNDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4753..5296
                     /gene="kdpC"
                     /locus_tag="OQQ48_000662"
                     /pseudo
     CDS             4753..5296
                     /gene="kdpC"
                     /locus_tag="OQQ48_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
     gene            5289..7973
                     /gene="kdpD"
                     /locus_tag="OQQ48_000663"
     CDS             5289..7973
                     /gene="kdpD"
                     /locus_tag="OQQ48_000663"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HCS1951535.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTDAMLEGLAVLPPKRLAYRGRHICEFDLDAALARR
                     PALILMDELAHSNALGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATIEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDTIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEEFHEDM"
     gene            7970..8647
                     /gene="kdpE"
                     /locus_tag="OQQ48_000664"
     CDS             7970..8647
                     /gene="kdpE"
                     /locus_tag="OQQ48_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HCS1951536.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVYEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFHLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
     gene            complement(8863..9120)
                     /locus_tag="OQQ48_000665"
     CDS             complement(8863..9120)
                     /locus_tag="OQQ48_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1951537.1"
                     /translation="MQKPLPVRWLIIQGLTENEHCGIQAYACNTESQEDLYAEVPLVG
                     RLTGIEEVKVEAIAMSKLHNMSCPPYMGHSAAVIFHLIYLS"
     gene            9243..11441
                     /gene="speF"
                     /locus_tag="OQQ48_000666"
     CDS             9243..11441
                     /gene="speF"
                     /locus_tag="OQQ48_000666"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HCS1951538.1"
                     /translation="MSKLKIAVSDSCPDCFTTQRECIYINESRNIDVAAIVLSLNDVT
                     CGKLDEIDATGYGIPVFIATENQERVPAEYLPCISGVFENCESRREFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEALFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIAEVAPQR
                     TKEARPFRLAVIQLGTYDGTIYNARQVVDKISHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGFDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQPQKEMFRKEHFPRV
                     SMNPQEANYAYSRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            11438..12776
                     /gene="potE"
                     /locus_tag="OQQ48_000667"
                     /pseudo
     CDS             11438..12776
                     /gene="potE"
                     /locus_tag="OQQ48_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
     gene            12852..13001
                     /locus_tag="OQQ48_000668"
                     /pseudo
     CDS             12852..13001
                     /locus_tag="OQQ48_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
     gene            13125..13775
                     /locus_tag="OQQ48_000669"
     CDS             13125..13775
                     /locus_tag="OQQ48_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001208812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2625 domain-containing protein"
                     /protein_id="HCS1951539.1"
                     /translation="MREMNQLVNTEDSAWPIIQNWLKDATNHTELLPVNKNLAETALY
                     QLQVTTKSPMGALVYGSGGLLIDNGWLRIAGSGHPRLPRDPVSWTQRPEFAGVRALPI
                     ADDVAGGIFALNGGDLGEDTGCVYYFAPDTLNWESLEVGYSEFLQWALSGDLDTFYEN
                     VRWQQWREDVIKLSATEAFTFYPFLWVQSEEARTRKVISLTELWEMQYQMKETFTQ"
     gene            complement(13816..14310)
                     /locus_tag="OQQ48_000670"
     CDS             complement(13816..14310)
                     /locus_tag="OQQ48_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1951540.1"
                     /translation="MKTNRSLVVIVSLITATLLLTACAQPEQSSLAGDWLLTPKDKTR
                     RLTGSIAVNIAPSRCKTNCRGDNLPDNTRRWQLSGGNEKELTYLHNMSAQEKVGLNPG
                     WQCYTSFFMRVCQGKPGTRPIVNEDYVSESGFFGSMMHVGIIELRRCQSENCQQELKA
                     INTH"
     gene            complement(14525..16165)
                     /gene="pgm"
                     /locus_tag="OQQ48_000671"
     CDS             complement(14525..16165)
                     /gene="pgm"
                     /locus_tag="OQQ48_000671"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HCS1951541.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALLADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(16191..16736)
                     /gene="seqA"
                     /locus_tag="OQQ48_000672"
     CDS             complement(16191..16736)
                     /gene="seqA"
                     /locus_tag="OQQ48_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HCS1951542.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIAEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            16921..17685
                     /gene="ybfF"
                     /locus_tag="OQQ48_000673"
     CDS             16921..17685
                     /gene="ybfF"
                     /locus_tag="OQQ48_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCS1951543.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     RDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPSGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            17825..18118
                     /gene="ybfE"
                     /locus_tag="OQQ48_000674"
     CDS             17825..18118
                     /gene="ybfE"
                     /locus_tag="OQQ48_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HCS1951544.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            18258..18788
                     /gene="fldA"
                     /locus_tag="OQQ48_000675"
     CDS             18258..18788
                     /gene="fldA"
                     /locus_tag="OQQ48_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HCS1951545.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            18998..19084
                     /gene="uof"
                     /locus_tag="OQQ48_000676"
     CDS             18998..19084
                     /gene="uof"
                     /locus_tag="OQQ48_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HCS1951546.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            19077..19523
                     /gene="fur"
                     /locus_tag="OQQ48_000677"
     CDS             19077..19523
                     /gene="fur"
                     /locus_tag="OQQ48_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HCS1951547.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(19608..19934)
                     /locus_tag="OQQ48_000678"
     CDS             complement(19608..19934)
                     /locus_tag="OQQ48_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1951548.1"
                     /translation="MKKLILIAIMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQHFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(19984..21390)
                     /gene="chiP"
                     /locus_tag="OQQ48_000679"
     CDS             complement(19984..21390)
                     /gene="chiP"
                     /locus_tag="OQQ48_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HCS1951549.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAVFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSLGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADGQQGYFLQRMTPAYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLLGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(21837..23501)
                     /gene="glnS"
                     /locus_tag="OQQ48_000680"
     CDS             complement(21837..23501)
                     /gene="glnS"
                     /locus_tag="OQQ48_000680"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HCS1951550.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTRQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSSEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(23704..25650)
                     /gene="nagE"
                     /locus_tag="OQQ48_000681"
     CDS             complement(23704..25650)
                     /gene="nagE"
                     /locus_tag="OQQ48_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HCS1951551.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPSTAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            25983..26783
                     /gene="nagB"
                     /locus_tag="OQQ48_000682"
     CDS             25983..26783
                     /gene="nagB"
                     /locus_tag="OQQ48_000682"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCS1951552.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            26843..27991
                     /gene="nagA"
                     /locus_tag="OQQ48_000683"
     CDS             26843..27991
                     /gene="nagA"
                     /locus_tag="OQQ48_000683"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCS1951553.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLARHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            28000..29220
                     /gene="nagC"
                     /locus_tag="OQQ48_000684"
                     /gene_synonym="nagR"
     CDS             28000..29220
                     /gene="nagC"
                     /locus_tag="OQQ48_000684"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HCS1951554.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            29268..30020
                     /gene="nagD"
                     /locus_tag="OQQ48_000685"
     CDS             29268..30020
                     /gene="nagD"
                     /locus_tag="OQQ48_000685"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HCS1951555.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETSSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            30417..32081
                     /gene="asnB"
                     /locus_tag="OQQ48_000686"
     CDS             30417..32081
                     /gene="asnB"
                     /locus_tag="OQQ48_000686"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HCS1951556.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSNQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            32462..32538
                     /locus_tag="OQQ48_000687"
     tRNA            32462..32538
                     /locus_tag="OQQ48_000687"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32496..32498,aa:Met,seq:cat)
     gene            32547..32631
                     /locus_tag="OQQ48_000688"
     tRNA            32547..32631
                     /locus_tag="OQQ48_000688"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32581..32583,aa:Leu,seq:tag)
     gene            32655..32729
                     /locus_tag="OQQ48_000689"
     tRNA            32655..32729
                     /locus_tag="OQQ48_000689"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32687..32689,aa:Gln,seq:ttg)
     gene            32764..32838
                     /locus_tag="OQQ48_000690"
     tRNA            32764..32838
                     /locus_tag="OQQ48_000690"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32796..32798,aa:Gln,seq:ttg)
     gene            32854..32930
                     /locus_tag="OQQ48_000691"
     tRNA            32854..32930
                     /locus_tag="OQQ48_000691"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32888..32890,aa:Met,seq:cat)
     gene            32979..33053
                     /locus_tag="OQQ48_000692"
     tRNA            32979..33053
                     /locus_tag="OQQ48_000692"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:33011..33013,aa:Gln,seq:ctg)
     gene            33091..33165
                     /locus_tag="OQQ48_000693"
     tRNA            33091..33165
                     /locus_tag="OQQ48_000693"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:33123..33125,aa:Gln,seq:ctg)
     gene            complement(33319..34494)
                     /gene="ubiF"
                     /locus_tag="OQQ48_000694"
     CDS             complement(33319..34494)
                     /gene="ubiF"
                     /locus_tag="OQQ48_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HCS1951557.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            34640..36064
                     /gene="miaB"
                     /locus_tag="OQQ48_000695"
     CDS             34640..36064
                     /gene="miaB"
                     /locus_tag="OQQ48_000695"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HCS1951558.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGSTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            36217..37257
                     /locus_tag="OQQ48_000696"
     CDS             36217..37257
                     /locus_tag="OQQ48_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCS1951559.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLTIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            37254..37721
                     /gene="ybeY"
                     /locus_tag="OQQ48_000697"
     CDS             37254..37721
                     /gene="ybeY"
                     /locus_tag="OQQ48_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCS1951560.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            37811..38689
                     /gene="corC"
                     /locus_tag="OQQ48_000698"
     CDS             37811..38689
                     /gene="corC"
                     /locus_tag="OQQ48_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HCS1951561.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            38714..40252
                     /gene="lnt"
                     /locus_tag="OQQ48_000699"
     CDS             38714..40252
                     /gene="lnt"
                     /locus_tag="OQQ48_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HCS1951562.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
BASE COUNT         9631 a        10364 c        10530 g         9940 t
ORIGIN      
        1 ggattgaccg gatggccgga cgtcaccccg ccggggcaca ccgaacaggc cacgccggtg
       61 ggggcaccaa tgcgcacccc ggctgaaccc tgaacaatgc taccgccata ctgcgtcatg
      121 tcgccctgac gcgctgccgg ttttccgctc atttctcatc cttgttttat cgggttattt
      181 gtccgctgac aggcaaaacc ctacagattt ataagaaagc cttaagtgag atatccatca
      241 gatagaaaca attaggaaaa tatttatata tttttcctga atatatttca gctgggtaat
      301 accatatcta aatatttacc tgaacatctt cctaataaaa aaaccgccag catctccact
      361 agcggttttc aatgtattca aaactcggca atcagtaaaa atcaccggtt gccttttcgg
      421 cctgttccat ccaaaccggt ttatcgctgg ttttcgacca gacgcggtgc aggtagctgt
      481 aaaagcgggc gcggtctttc cagaaaagca tgaaaggcag cgccagaacg cccgctgcaa
      541 ccgcatatgt acggcgtaaa aagataagcc atgcaggata ttctctgtag agttccatac
      601 ttttctcccc cttttgtatc tacccggtga atagcaccgg aaaaatgaat ttgtttatct
      661 gatgaaaata gtaccgcctt ttgtgtaatt ttactactca tccgaccact tatttttgct
      721 tattgatggt ttatttacat tcactctgta attaagttgc acaaaagtta aattaatgct
      781 aaacattagt taaaccatgg cttttgccat ttttatactt tttttacacc ccgcccgcag
      841 atttttgcga aatctttgca gccagaattc tactcttccg gtatcacttt taggccactg
      901 gaggtgcact gtgagtgcag gcgtgatagc cggcgtgttg ctggtgtttt tattactggg
      961 ttatttggtt tatgccctga tcaatgcgga ggcgttctga tggctgcgca agggttctta
     1021 ctgattgcca cgtttttact ggtattaatg gtgctggcgc gtcctttagg cagcgggctg
     1081 gcgcggctga ttaatgacat tcctcttcca ggtacagcgg gcgttgaacg tatactcttt
     1141 cgtttacctg gcgtctctga ccatgagatg aactggaagc aatatctttg tgccattctc
     1201 ggcctgaaca tgctggggct ggcggtgctg ttttttatgt tgctcggtca gcactatctg
     1261 ccgcttaatc cacagcagtt gccggggctg tcgtgggatc tggcgctgaa taccgccgtc
     1321 agctttgtca cgaataccaa ctggcaatct tatagcggtg aaaccacgtt gagctatttc
     1381 agccagatgg caggcttaac ggtacaaaac tttctttctg ccgccagcgg gattgcggtg
     1441 atttttgcct tcatccgtgc gtttactcgc cagagcatga gcacactcgg gaatgcctgg
     1501 gtcgatctgc tacgcatcac gttatgggtg ctagtccctg tggcgttgtt gatcgcactg
     1561 ttttttattc aacaaggtgc gctgcaaaac tttctgccct atcaggcagt gaataccgtt
     1621 gaaggagcgc aacagctgtt acccatggga ccagtagctt ctcaggaagc gatcaagatg
     1681 ctcggtacta acggcggtgg cttctttaat gccaactcgt cgcatccgtt tgaaaaccca
     1741 accgcactga ccaacttcgt gcagatgctg gcgatcttct tgatcccaac agctctgtgc
     1801 tttgcctttg gtgaagtgac gggcgatcgc cgccaggggc gcatgttgct gtgggcgatg
     1861 tcagtgattt ttgtcatctg cgtaggcgtg gttatgtggg cagaagttca gggtaatcct
     1921 catctgctgg cgctcggcgc agacagcagc atcaatatgg aaggtaaaga gagccgtttc
     1981 ggcgtgctgg tcagtagcct gtttgcggtc gtgactacgg cggcgtcctg tggcgcggtg
     2041 attgcgatgc atgattcgtt taccgctctc ggcggcatgg tgccgatgtg gctaatgcaa
     2101 attggtgaag tggtgttcgg cggtgtcggt tctggtcttt acggcatgat gctgtttgtc
     2161 ctgctggcgg tgtttattgc cgggctgatg attggtcgta caccggaata tctgggtaaa
     2221 aaaatcgacg tacgcgagat gaaactgact gcactggcga ttctggttac tccaacactg
     2281 gtactgatgg gcgcggcgtt ggcgatgatg accgacgccg gacgtagcgc catgcttaac
     2341 cctggcccgc atggttttag cgaagtgctg tacgccgtgt cgtccgccgc taacaacaac
     2401 ggcagcgcct ttgccggatt aagcgccaac tctccgttct ggaactgttt actggcgttc
     2461 tgcatgtttg tcggtcgctt cggggtgatt atcccggtga tggcaattgc cggttcgctg
     2521 gtgagtaaaa agagccaacc cgccagctcc ggcacgctgc caacgcacgg cccgctgttt
     2581 gttggcctgt taatcggcac cgtgttgctg gttggcgcac tgacctttat ccctgccctg
     2641 gcgcttggtc cggtggcgga atatctctcc tgatgatatt gagtgagcac tgaatatgag
     2701 tcgtaaacaa ctggcgctat tcgaaccaac acttgtcgtt caggcgctga aagaagcggt
     2761 gaaaaaatta aacccgcagg cgcaatggcg caatccggtg atgtttatcg tctggatcgg
     2821 cagtctgctg accacctgta ttagcatcgc gatggcaagc ggtgcgatgc ctggcaatgc
     2881 gctgtttagc gcggcaatta gcggctggct gtgggtcacc gtactgttcg ctaatttcgc
     2941 cgaggcgctg gcagaaggcc gcagtaaagc gcaggccaac agtcttaaag gggtgaaaaa
     3001 aactgccttt gcccgcaagc tgcgtgagcc gaaatatggc gctgcggcgg acaaagttcc
     3061 tgccgatcaa ctgcgtaaag gtgacatcgt gctggtagaa gctggcgata ttatcccctg
     3121 cgatggtgaa gtcattgaag gcggcgcatc ggtcgatgaa agcgccatca ccggggaatc
     3181 ggcaccggtg atccgtgaat ccggcggcga ttttgcctcc gtcaccggcg gcacgcgtat
     3241 tctttctgac tggctggtga ttgagtgtag cgttaacccc ggtgagacat ttctggatcg
     3301 gatgatcgcg atggtggaag gcgcacagcg acgcaaaaca ccgaacgaga ttgccctgac
     3361 tatcctgctg attgctctga ccatcgtctt tttactggca accgccacgc tgtggccgtt
     3421 ttccgcgtgg ggcggtaatg ccgtgagtgt aacggtactg gtggcgctgc tggtctgtct
     3481 gatcccaacc actattggcg gcctgttgtc agcgatcggc gtcgccggga tgagtcggat
     3541 gctgggcgcg aatgtgattg ccaccagcgg acgtgcagtt gaagcggcag gtgacgttga
     3601 cgttctgcta ctggataaaa ccggcaccat cacactcggt aaccgtcagg cgtcggagtt
     3661 tatccctgcg cagggcgtgg atgaaaaaac gctggctgac gccgcacaac tggcttcgct
     3721 ggctgatgaa acgccggaag gccgcagtat tgtgatcctc gccaagcaac gttttaacct
     3781 gcgcgagcgc gatgtgcagt cgctccatgc cacctttgta ccgtttactg cgcaaagccg
     3841 gatgagcggg atcaacatcg acaaccgcat gatccgtaaa ggttctgtcg atgccattcg
     3901 ccgccatgtt gaggctaacg gcggtcactt ccctgccgat gttgatcaaa aagtcgatca
     3961 ggttgcgcgt cagggggcca cgccgctggt ggtggtggaa ggttcccgtg tgctgggcgt
     4021 tattgcgctg aaagatatcg tcaaaggcgg tattaaagag cgcttcgccc agctgcgcaa
     4081 aatgggcatt aaaacggtga tgattaccgg cgataaccgt ctgactgccg ccgccattgc
     4141 tgcggaagcg ggcgtcgatg attttctcgc cgaagcgaca ccggaggcca agctggcatt
     4201 gattcgtcag tatcaggcgg aaggccgttt ggtggcgatg accggcgacg gcaccaacga
     4261 tgctccggcg ctggcgcaag cagatatcgc ggtggcgatg aactccggca cccaggcggc
     4321 gaaagaggcg ggcaatatgg tcgatctcga ctctaacccg accaagttga tcgaggtggt
     4381 gcatattggc aaacagatgc tgatgacccg tggctcgctg accaccttca gcattaccaa
     4441 cgatgtggcg aaatacttcg ccattattcc ggcggcgttc gcggcaacgt atccgcagtt
     4501 aaatgcgctg aacatcatgc gcctgcattc gcccgactcc gccattctca gtgcagtcat
     4561 tttcaacgcc ttgattatcg tctttttgat tcctctggcg ttaaaaggcg tgagttataa
     4621 accgcttacc gcttctgcca tgttgcgccg taacttatgg atttacggtc tgggtgggct
     4681 gctggtgccg tttattggta tcaaagtcat tgatttactg ctgaccgttt gcggtctggt
     4741 gtgaggttta ccatgcgtgg cttacgtccg gcattatcga catttatctt tctgttattg
     4801 attactggcg gcgtttaccc gctgctgacc accgtgctgg ggcaatggtg gtttccctgg
     4861 caggccaacg gttcgttgat tcgtgaaggt gatacggtgc ggggttcggc attaatcggg
     4921 cagaatttta ccggcaacgg cagaaatgcc ctataatcca caggcttctg gcgggagcaa
     4981 tctggcggtc agtaaccctg agctggataa acaaatagcc gcacgcgttg ctgcattacg
     5041 ggccgctaac ccggatgcca gtacgaacgt tccggttgaa ctggtgacgg catcggcaag
     5101 cgggctggac aataatatca ccccgcaagc ggcggcctgg cagatcccac gcatcgcgaa
     5161 agcgcgtaat ctcagcgttg aacagctcac gcagctgatc gcaaaataca gccaacaacc
     5221 gctggtgaaa tatatcggcc agccggtcgt caacattgtc gaactcaatc tggcgctgga
     5281 taagcttgat gaataacgaa cccttacgtc ccgaccccga tcgtctgctg gaacaaactg
     5341 ccgcgccgca tcgggggaag ctgaaagttt tcttcggtgc ctgtgcaggc gtcgggaaga
     5401 cctgggcgat gctggcagaa gcccagcgac tgcgggcgca agggctggat attgtggttg
     5461 gcgtggtaga aacccacggg cgaaaagata ccgacgcaat gctggaaggg ctggctgttc
     5521 tgccgccaaa acgcctggcg taccgtgggc ggcacatctg cgagtttgac ctcgatgccg
     5581 ccctcgcccg ccgtccggcg ctgatcttaa tggacgaact ggcgcacagt aatgcgctag
     5641 gttctcgtca ccctaaacgc tggcaggaca tcgaagagct gctggaagca ggcattgatg
     5701 ttttcactac cgtcaacgtt cagcatctgg aaagtctgaa tgatgtggtc agcggcgtca
     5761 ccggaattca ggtacgggaa accgtgcccg atcctttttt cgatgccgcc gacgacgtgg
     5821 ttctggtgga cttgcccccg gacgatctgc gccagcggct gaaagaaggc aaagtctata
     5881 ttgccgggca ggcggagcgc gccattgaac attttttccg caaaggcaat ctgatcgccc
     5941 tgcgcgaact ggcactgcgc cgtactgccg atcgcgttga tgagcaaatg cgcgcctggc
     6001 gggggcatcc tggcgaagag aaagtctggc acacgcgcga cgcgatcctt ttatgcatcg
     6061 gccataacac cggtagtgag aagctggtcc gcgcggcggc gcggctggcg tcacggctgg
     6121 gtagcgtctg gcacgcggtg tatgttgaaa cccctgccct gcaccgctta ccggaaaaaa
     6181 aacgtcgggc aattctcagc gccttacgtc tggcgcagga gctgggcgcg gagacggcaa
     6241 cactttctga tccagcggaa gaaaaagcag tagtgcgtta tgcccgtgaa cataatctcg
     6301 gcaagattat tctcggtcgc ccggcctcgc gccgctggtg gcgtcgggaa acgtttgctg
     6361 accgactggc gcgcatcgcc cccgatctcg atcaggtgct ggtcgcgctt gatgaaccac
     6421 ccgcccgcac gattaacaac gcgccggata gccgctcttt taaagacaag tggcgggtac
     6481 agattcaggg atgcgtggtt gccgccgcgt tatgcgccgt tatcacttta attgccatgc
     6541 agtggctgat ggcgtttgat gccgccaacc tggtgatgct gtatctgctt ggcgtggtgg
     6601 tggtggcgct attttatgga cgctggcctt cggtggttgc taccgtcatt aatgtggtga
     6661 gtttcgatct cttttttatc gccccacgcg gcacgctcgc cgtctctgat gtgcaatatc
     6721 tgctgacctt cgcagtgatg ttaaccgtcg ggctggtgat cgggaacctt actgctggcg
     6781 tgcgttatca ggcgcgggta gcccgttacc gcgagcaacg cacacggcac ttatatgaaa
     6841 tgtcgaaagc tctggcggtg ggccgcagtc cgcaggacat cgccgccacc atcgaacaat
     6901 ttattgcctc cacgtttcat gcccgcagtc aggtgttgtt gcccgatgac aacggtaaat
     6961 tgcagccgtt aacacatccg caaggaatga cgccatggga cgataccatc gcgcagtgga
     7021 gttttgataa aggcctgcct gcgggcgcgg gcaccgacac gttacccggt gtaccgtacc
     7081 agattttgcc gctaaaaagc ggcgagaaaa cctacgggct ggtggtagtg gagccgggga
     7141 atctgcgcca gttgatgatc ccggaacagc agcgcctgct ggagacgttt acgctgttag
     7201 tcgccaatgc ccttgagcgg ctgacgctaa ccgccagcga agaacaggcg cggatggcaa
     7261 gcgaacgtga acagatccgc aacgccctac tggcagcgct ttcgcatgat ttacgcacgc
     7321 cgctcacggt gctgttcggt caggcagaaa tcttaacgct cgatctggca agcgaaggat
     7381 cgccccacgc ccgccaggcc agcgagattc gtcagcatgt gctgaacact acccgactgg
     7441 tgaataatct actggatatg gcgcgaattc agtccggcgg ctttaatttg aagaaagagt
     7501 ggttaacgct ggaagaagtg gtcggtagcg cgctgcaaat gctggaaccg ggtttatcgt
     7561 cgcccatcaa tctttctctg ccagaaccgc tgaccttaat ccacgttgac gggccactct
     7621 ttgaacgggt gctgattaat ctgctggaga acgcggtgaa atatgcgggt gcgcaggccg
     7681 aaattggtat cgatgcccac gttgagggcg aaaatctaca actggatgtc tgggataacg
     7741 gccccggtct tccgccaggc caggagcaga cgatatttga taagtttgct cgcgggaata
     7801 aagagtcggc agtaccgggg gtagggcttg gactggcaat ttgtcgggcg atagtggatg
     7861 tacacggggg cactattacc gcgttcaacc gaccggaagg tggtgcctgt tttcgtgtta
     7921 cacttcccca gcaaactgcc cctgaacttg aagaatttca tgaggatatg tgacaaacgt
     7981 tctgattgtt gaagatgaac aggctattcg tcgctttctg cgcacggcgc tggagggcga
     8041 cgggatgcgc gtctatgagg ccgaaacgct gcaacgcggc ttgctggaag cggctacccg
     8101 taagccagat ttgattattc tcgatctcgg cctgcccgat ggtgatggga ttgagtttat
     8161 ccgcgacctg cgccagtgga gcgcggtgcc ggtgattgtg ctttccgcac gcagcgaaga
     8221 gagcgacaaa atcgccgcgc tggatgccgg agcggatgat tatctgagta agccgtttgg
     8281 cattggcgaa ttgcaggccc gtctgcgcgt cgctttacgc cgccactccg ctaccgccgc
     8341 gcccgatccg ctggtgaaat tttccgatgt taccgtcgat ttagccgccc gcgtgattca
     8401 ccggggtgag gaagaggtgc atctcacacc aattgagttc cacctgctgg cggtgctgct
     8461 caacaatgcc ggaaaagtac tcacccagcg ccagctcctt aaccaggtgt gggggccaaa
     8521 cgcggtcgaa cacagtcact atttgcgtat ttatatggga catctgcgac aaaaactgga
     8581 acaggatccc gcccgcccac gccatttcat tactgaaacc ggtattggct atcggtttat
     8641 gctttgaata ttaattttaa tacagcctgc cttttattaa ttaaagccgt aataataaat
     8701 acggcttttt atcttaaaca acacacaaaa ataacaattc aatattttat attactgagt
     8761 aaaaagctca tcattaaata aattaagatt gatcattttt tattgatcac cttcacagtt
     8821 caaccgtatt tcctggatag aatatcttca ccttcattca catcaggaaa ggtaaattaa
     8881 atggaaaata acagccgcac tatgccccat ataaggcgga caactcatat tatgaagttt
     8941 gctcatcgca atagcttcga ctttcacttc ttcaatgccc gttagtctac cgactaaggg
     9001 cacttcagcg tacaggtctt cctgactctc tgtattacag gcatatgcct gtattccgca
     9061 gtgctcattc tctgttaacc cttgtattat cagccaccgg actggtaagg gtttctgcat
     9121 tctgaaaaaa gcaatctacc tgatttattt atcagcgggg taactttgct tttttccggc
     9181 acgatcgatt tctcattcga gaaattgagg acctgctatt acctaaaata aagagatgaa
     9241 aaatgtcaaa attaaaaatt gcggttagtg attcttgccc ggactgtttt accacgcagc
     9301 gagaatgtat ctacattaat gaaagtcgta atatcgatgt ggcggcaatc gttttatcgc
     9361 tcaacgatgt tacatgcgga aaactcgatg aaatcgatgc cacgggttat ggcatcccgg
     9421 tatttattgc tactgaaaat caagaacgtg tacccgcaga gtatttgccc tgtatttcgg
     9481 gtgtctttga gaattgcgaa tcgcgacgag aattttatgg tcgtcagtta gaaaccgctg
     9541 ccagccatta tgaaactcaa ctgcgcccac ctttcttccg cgcactggtc gattatgtca
     9601 atcaaggtaa cagcgcgttt gattgccctg gtcatcaggg cggcgaattt ttccgtcgcc
     9661 atccggcggg gaatcagttt gtggaatact ttggtgaggc gctgttccgt tccgacttgt
     9721 gcaacgcaga cgtagcaatg ggtgatctgc tgattcacga aggcgcgcca tgcattgcac
     9781 agcaacatgc ggcaaaagtg ttcaatgccg ataaaacata cttcgttcta aatggcactt
     9841 catcttctaa caaagtggtt ttaaacgccc tgctgacacc gggtgatctg gtgctgtttg
     9901 accgcaataa ccacaaatct aaccaccacg gagcgttgct acaggctggt gcaacaccgg
     9961 tttatctgga aaccgcacgt aacccgtatg gctttatcgg tggcattgat gcgcactgtt
    10021 ttgaagaaag ttacctgcgt gagctgatcg cggaagtcgc accgcagcgg acaaaagagg
    10081 ctcgtccttt ccgcctcgcc gttattcagc taggcaccta cgacggtacg atttataacg
    10141 cccgccaggt ggtggataaa attagtcatc tgtgtgacta cattctgttt gactccgcat
    10201 gggtcggcta tgagcaattt atcccgatga tggctgactg ttcaccgctg ttgctggatc
    10261 ttaatgagaa cgatccgggc attctggtta cgcaatctgt gcataaacag caggctggtt
    10321 tttctcaaac ttcacaaatc cataaaaaag acagccacat caaagggcaa cagcgttatg
    10381 taccgcacaa acgcatgaac aacgccttta tgatgcatgc ctccaccagc ccgttctatc
    10441 cgctgtttgc cgcactggat atcaacgcca aaatgcatga aggtgtcagc ggtcgtaata
    10501 tgtggatgga ttgtgtggta aatggcatta atgcccgcaa gctgatcctc gataactgtc
    10561 agcatattcg tccgttcgta cctgaactag tggatggtaa accctggcag tcgtatgaaa
    10621 cagcgcaaat tgcggttgat ctgcgcttct tccagtttgt accaggggaa cactggcact
    10681 cttttgaagg ctatgcagag aatcaatact ttgtcgatcc atgcaaactg ttgctgacaa
    10741 ccccaggttt tgatgcacgt aacggcgaat atgaagcgtt cggtgtaccc gcgacgattc
    10801 ttgctaactt cctgcgcgaa aatggcgtag tgccggaaaa atgcgatctt aactccatcc
    10861 tcttcctgct gactccggca gaagatatgg ccaaacttca gcaacttgtt gccctgctgg
    10921 tacgcttcga aaaactgctt gagtccgacg cgccattagc agaagtgctg ccttccatct
    10981 acaaacagca tgaagagcgc tacgccggtt atacccttcg tcagttatgc caggaaatgc
    11041 atgatttgta tgcccgtcac aacgtgaaac aaccgcaaaa agagatgttc cgtaaggaac
    11101 acttcccacg cgtaagcatg aatccacaag aagccaacta cgcctattca cgcggtgaag
    11161 tggagctggt tcgtctgccg gatgccgaag gccgtatcgc tgccgaaggt gcgcttcctt
    11221 atcctccggg tgtgctgtgt gttgttccgg gtgaaatctg gggtggtgct gttttgcgtt
    11281 acttcagcgc tctggaagaa gggatcaacc tgctgccagg ttttgcaccg gagctgcagg
    11341 gtgtctatat cgaagaacat gatggtcgta agcaagtttg gtgctatgtc atcaagcctc
    11401 gtgatgcgca aagcaccctg ttgaaagggg aaaaattatg agtcaggcta aatcgaacaa
    11461 aatgggcgtc gttcagttaa ccatactgac gatggtcaac atgatgggct ccggtatcat
    11521 catgctgccg acaaagcttg ccgaagttgg gacaatctca attatctcct ggctggtgac
    11581 agccgtaggc tcaatggcac tggcatgggc attcgcaaaa tgcggtatgt tcagccgtaa
    11641 atcaggcggt atgggcggtt atgcggagta tgctttcggt aagtccggca attttatggc
    11701 gaactacacc tacggtgtct cgctgctgat tgctaacgtc gcgattgcta tttcggcggt
    11761 tggttacggc accgaactgc tcggcgcgag tttgtcgcca gtgcagattg gtcttgcgac
    11821 catcggagtg ctgtggattt gtaccgtggc taactttggt ggtgcgcgca ttaccgggca
    11881 aatcagtagc attactgtgt ggggagtcat tattccggtc gtcggtctgt gcattattgg
    11941 ctggttctgg tttagtccga cgctgtacgc taactcatgg aacccgcacc atgtgccgtt
    12001 ctttagcgct gtaggttctt ccatcgccat gacgttgtgg gctttcctcg gtctggagtc
    12061 agcctgtgcc aacgccgaag tagtggaaaa cccggaacgt aacgtgccga ttgcagttct
    12121 tggcggtact ttaggtgcgg cggtgattta tatcgtctcc accaacgtga ttgccgggat
    12181 tgtgcctaat atggagctgg caaattcaac ggcaccattt ggcctggctt tcgcacagat
    12241 gttcacacca gaagtgggta aagtcattat ggcgctgatg gtgatgtcct gctgcggttc
    12301 actgcttggc tggcggttca ctgcttggct ggcagttcac cattgcccag gtgtttaaat
    12361 cttcatctga tgaaggctac ttccctaaaa ttttctcccg tgtaaccaaa gtggatgcac
    12421 cggtgcaggg aatgttgacc attgtgatta ttcagagtgg attggcactg atgaccatta
    12481 gcccgtcact gaacagccag ttcaacgtgc tggttaacct ggccgtggtg accaatatca
    12541 ttccgtatat tctgtcaatg gcggcattag tcattattca gaaggtcgct aatgtgcccc
    12601 cctcaaaagc gaaagtagca aactttgttg cctttgttgg tgcgatgtac agtttctatg
    12661 cgctgtactc atccggggaa gaagccatgc tgtacggttc tatcgtgacc ttccttggtt
    12721 ggacactgta tggtctggta tcaccatgct ttgaactgaa aaataaacac ggttaataaa
    12781 acaaagtaaa aaagggcgat tgaatgatcg ccctttctgt ttgttggcaa atagttcgtt
    12841 atttatgccg catccggcag atgtagggtc taaacgcgat cgaacacagc aactatttgc
    12901 gtaattatat ggggcatctg cgacaaaaac tggaacagga tccggcccgg cctcgccatt
    12961 ttattactga aaccggtatt gggtatcggt ttatgctttg aaaaaagtgg caatgtatgg
    13021 ataaagtcat tttttattca aatataagga acttaatatt taaaaaatgt tccatacaat
    13081 tcctcatcat aaatgacacc gtagcacaca aggagcaagt agcgatgcgt gaaatgaatc
    13141 agttagtaaa tactgaagat tcagcctggc caataatcca gaactggtta aaagacgcca
    13201 caaaccatac cgaactctta ccggttaata aaaatttagc cgaaaccgca ctttatcaat
    13261 tacaggtgac aacaaaatca ccgatgggtg cgcttgtcta tggctccgga ggtctgctga
    13321 ttgataatgg ttggttacgc atagcaggat ctggtcaccc ccggttgcca cgagatccag
    13381 taagttggac acaacgccct gaattcgcag gcgtgcgggc tttacccatc gctgacgatg
    13441 tcgcaggggg aatttttgct cttaatggtg gcgatcttgg ggaagatact ggctgcgttt
    13501 attattttgc gccagatacg ctgaattggg aaagtctgga agttggatat tctgagtttc
    13561 ttcagtgggc attatccggt gatttagata ccttctatga aaatgtacga tggcagcaat
    13621 ggcgagaaga cgtaataaaa ttatcggcaa cggaagcctt cacgttctac cctttcttat
    13681 gggtacaaag tgaagaagca agaacaagaa aagttatttc gcttactgaa ctctgggaaa
    13741 tgcagtatca aatgaaagaa acattcacgc aataagacat gttcaggtcc gggtttcatt
    13801 cccggactca ctattttaat gtgtatttat cgctttcaat tcttgctggc aattttctga
    13861 ctggcagcgt cgtaactcaa taatcccgac atgcatcatc gagccaaaaa atccgctttc
    13921 gctgacataa tcctcattga ctatcggtcg agtgccgggc ttgccctggc aaacccgcat
    13981 aaaaaatgag gtgtagcatt gccaaccggg atttaaaccg accttttctt gtgctgacat
    14041 attatgaaga taagtaagtt ctttttcgtt cccgcctgac aactgccagc ggcgggtatt
    14101 atcaggtaag ttatcaccac ggcaatttgt cttacaccgg gaaggtgcaa tatttaccgc
    14161 aatgctaccc gttaatcttc tggttttatc tttaggggtc agtaaccagt cgccagctaa
    14221 tgatgattgt tcaggttggg cgcatgcagt cagcagtaat gtggcggtaa ttagtgacac
    14281 tatgacgacc agactcctgt tggtcttcat tgggatttcc ttgagctact cagtttgttg
    14341 acaaagtgtt cgttgttttt gccggatgcg gcgtgaacgc cttatccggc ctacaaaatc
    14401 tcgtaaattc aatatattgc accatcctcg taggcctgat aagcgtagcg catcaggcaa
    14461 ttctgtgttt gtcatacgta aaaaagggcg atcttccgac cgcccttttt cttatttaat
    14521 gtgtttacgc gtttttcaga acttcgctaa caatctcaac cgcttctttc tcaatctgct
    14581 tgcgatgttc ttcaccgagg aagctttcgc agtagatctt atatgcgtct tccgtgcctg
    14641 acggacgcgc ggcgaaccag ccgttgtcag tcatcacttt cagaccgccg atagaagcac
    14701 cattgcccgg tgccgccgtc aggcgcgcgg tgatcgggtc gcctgccagg gtgctggcgc
    14761 tcaccatttc cggagacagc ttagacagcg ccgctttttg tgcggaagtc gcagctgcct
    14821 gcaaacggtt gtagctcggc gcaccaaagc gttttgccag ttcgttgtag tgttcctgcg
    14881 ggttcttacc ggtgacagcg gtgatttccg ccgccagcag acacatgatg atgccgtctt
    14941 tgtcggtgga ccacggcgtg ccgtcgaaac gcaggaagga agcccctgcg ctctcttcgc
    15001 cgccaaagcc gaagctgccg tcgaacagac catcaacaaa ccatttgaaa cctaccggga
    15061 cttctaccag cttgcggccc aagtcattga ccacacggtc gatcatcgca gaagaaacca
    15121 gcgttttacc gacagcaaca tctttgcccc actgcggacg atgctggaac aggtagttga
    15181 tcgccaccgc caggtagtgg ttcggattca tcaaacctgc cggagtgacg ataccgtgac
    15241 ggtcataatc cgggtcgtta gcaaacgcca gatcgaactt atcacgcagt gccagcaggc
    15301 ccgccatcgc acactcggag gagcagtcca tacggatcgc gccgtcttta tcaaggtgca
    15361 taaagcggaa ggtttgatcg acctgatcgt taacgatggt caggttgagg ttgtaatact
    15421 cgccaatacg cttccagtat tcgataccgg aaccgcccag cggatcaacg cccagcgtca
    15481 ggcccgcttt ctgaatcgcc gccatatcaa cgatatcggc cagaccttcc acaaacggct
    15541 gcaccagatc ctgctctttc acatgaccgg atgccatcgc ttcgtcgagg gagatacgct
    15601 tcacgccttt caggccatcg gccagcagtg cgttggccct gtcttccacc actttggtga
    15661 cgttggtatc agccgggcca ccatttggcg gattgtactt gataccacca tcttccggcg
    15721 ggttatggga cggtgtaatc acgataccgt ctgccagcgg accacctttt ttattgtgaa
    15781 ccaggatggc attggaaacg gcaggcgttg gagtgaagcc attgttttcc tgcacaatga
    15841 catcaacgcc gttcgctgcc agcacttcca gtacggaaat gaacgcaggc tcggacaggg
    15901 cgtgagtatc tttacccaca tagcaagggc cagtgatgcc gtttttcgca cgttcttcag
    15961 caattgcctg agcgatcgcc agaatgtgcg gctcgttaaa gctgtggcgc gctgcactgc
    16021 cacggtgacc ggaagtaccg aatttcaccg cgtgctccgc attccctgct tctggtttca
    16081 gtacataata ttgcgccgtc agttgggcga cgttaatcaa atcactctgt tgtgcaggtt
    16141 ggcctgcacg attgtggatt gccattgctt tgtcctttgt ctgcaacgtt ttagatagtt
    16201 ccgcaaacct tctcaatcaa ttccgccggg aattgcatcg actgcatgat gtgttcgatc
    16261 atgctgcatt tacggccggt gttggtgttg gtgatcaccc aatacggcgt gcctggcaca
    16321 tgtttcggct tggtctgatt accatttttc agcagcgttt gttcatctgc cgcaaagtaa
    16381 acgcgtgtac gaccgtgcaa cgattccgtt gcttcggcaa acgcctgggc gtcaagagaa
    16441 tatagtgtag acaacagcag cataaagcga ttgaccgctc gcttttgctc tgcgtattcg
    16501 tcagaaagca gaagttcacg cattgcgcga accttgtctt taatcgtttt gaccggcttc
    16561 gcttcggcga tagcaggtga cgcaacgcga acctctttcg tcaccggagc agcaggctgt
    16621 gatgcggcgg aaaatttcaa catacgccgt aaaatgtcgg atgcgctctc gccgatatgc
    16681 ttagtgtggc tggcaatata gctgtagagt tcatcatcaa cttcaatcgt tttcatctta
    16741 atccagtgca gtgtcttagc tgaatacaag tcgccaagag tatatgaata aatcccggca
    16801 gcggatagcg tcaagcctgg cttgcagaaa aactcggaat aaaccttatt tgttgcagcc
    16861 ctgtggcaca atggtcaaca taatacccta acccgacaac gcgaaaaaaa gaactttgcc
    16921 atgaaattga atatccgcgc gcaaactgca caaaaccagc acaataattc tcccatcgtt
    16981 cttgtccatg gtctgtttgg tagcctcgac aaccttggcg tactggctcg cgatctggta
    17041 aacgatcaca atatcatcca ggttgatatg cgtaaccacg gtctttcacc gagagatccg
    17101 gtaatgaatt acccggcgat ggcgcaggat cttgttgata ctctggatgc acagcagatc
    17161 gacaaagcaa catttatcgg tcactccatg ggcggtaaag cggtaatggc acttacggca
    17221 ctggcccgcg atcgcatcga taaactggtg gcgatcgata tcgcaccggt cgactatcac
    17281 gtacgccgtc atgatgagat tttcgcggct atcaacgcgg tcagtgaatc ggacgcacaa
    17341 actcgccagc aagcagcagc aataatgcgc cagcatctta atgaagaagg ggtgattcag
    17401 tttctgttga aatcttttgt tgacggggag tggcgcttta acgtgccggt attgtgggat
    17461 cagtatccgc atattgtggg ttgggagaaa atcccggcat gggatcaccc tgccctgttt
    17521 atccctagcg gcaattctcc gtatgttagc gagcagtacc gtgatgattt actggcacaa
    17581 tttccacagg cacgggcgca tgtgattgca ggcgcgggtc actgggtcca tgctgaaaaa
    17641 ccggatgcgg tattacgcgc tatccgtcgc tatctcaatg attaacgcaa ctgattaaaa
    17701 acccagcgtc cgcgtcgctt tccgcgcgtg ggcgttggcg tgactcatcg cgctgatgta
    17761 ttatggcgcg ctatccatcc gggcagaggc ctggctgatt gtttcccccg aagtcaccaa
    17821 gatcatggcc aaagaacaaa cggaccgtac gacattagat ctgttcgcgc acgagcgtcg
    17881 accgggacga ccgaaaacta atccgctttc gcgcgatgaa cagctgcgta ttaataaacg
    17941 caaccagcta aaacgcgaca aagtacgtgg ccttaagcgt gtcgaactga agctgaacgc
    18001 ggaagctgtc gaggcgctga acgagctggc ggagtcgcgc aatatgagcc gtagcgaact
    18061 gatcgaagag atgttaatgc agcaactggc ggcgctgcgt agtcagggca tcgtttaaat
    18121 ttccactttc atgtagcaca gtgtgcagtc ctgctcgttt gctgattccg aaagactgcc
    18181 tgttctgcta tgattgcctt tatccgtggg caattttcca cccccatttc aataagtttc
    18241 aagaggttat ttcactcatg gctatcactg gcatcttttt cggcagcgac accggtaata
    18301 ccgaaaatat cgcaaaaatg attcaaaaac agcttggtaa agacgttgcc gatgtccatg
    18361 acattgcaaa aagcagcaaa gaagatctgg aagcttatga cattctgctg ctgggcatcc
    18421 caacctggta ttatggcgaa gcgcagtgtg actgggatga cttcttcccg actctcgaag
    18481 agattgattt caacggcaaa ctggttgcgc tgtttggttg tggtgaccag gaagattacg
    18541 ccgaatattt ctgcgacgca ttgggcacca tccgcgacat cattgaaccg cgcggtgcaa
    18601 cgatcgttgg tcactggcca actgcgggct atcatttcga agcatcaaaa ggtctggctg
    18661 atgacgacca ctttgtcggt ctggctatcg acgaagaccg tcagccggaa ctgaccgctg
    18721 aacgtgtaga aaaatgggtt aaacagattt ctgaagagtt gcatctcgac gaaattctca
    18781 atgcctgatg tgatgcggcg tagactcatg tctacgccgt attaatagat aatgccaatc
    18841 aaaataattg ctacaaattt gtaacttttg ctgttgtacc tgtacaatgt cccggtgttc
    18901 aagtggcctt gccgttgtaa atgtaagctg tgccacgttt ttattaacaa tatttgccag
    18961 ggacttgtgg ttttcattta ggcgtggcaa ttctataatg atacgcatta tctcaagagc
    19021 aaattctgtc acttcttcta atgaagtgaa ccgcttagta acaggacaga ttccgcatga
    19081 ctgataacaa taccgcccta aagaaagctg gcctgaaagt aacgcttcct cgtttaaaaa
    19141 tcctggaagt tcttcaggag ccggacaacc atcacgtcag tgcggaagat ttatacaaac
    19201 gtctgatcga tatgggtgaa gaaattggtc tggctacggt atatcgcgta ctgaaccagt
    19261 ttgacgacgc tggtatcgtc acccgccaca attttgaagg cggtaaatcc gtatttgaac
    19321 tgacacagca acatcaccac gatcacctga tctgcctcga ctgcggcaag gttatcgaat
    19381 ttagtgatga ttccatcgaa gcgcgtcagc gtgaaattgc cgcaaaacat ggcattcgcc
    19441 tgactaacca cagtctctat ctttacggtc actgtgccga aggcgattgc cgcgaagatg
    19501 aacatgcgca cgaaggcaaa taagccagcc tgaatgagaa aaagccaacc tgcgggttgg
    19561 cttttttatg caagggaaat aagaaaagcg agagttacag ctctcactta tttgttatta
    19621 ctgcgaattt cctgccaaac tttatcacaa tcggctttca ccgcctgatc atttccggtt
    19681 tgcgttgcac gcagacactg ctgataatcc agtacgcgga cactttcctg gtcggcaaaa
    19741 tgctgatgtt gtttctcttt cttcagcacg tttaacacgc tctggcaggc ttcaattttt
    19801 tctggcgaac cttgtgcggt gttgatacag gcgctgtatg cctctttcag acgactgtct
    19861 tcctgtggcg cggttgattg cgcacaagct accagccccg atgccattat ggcgatgaga
    19921 atcagttttt tcatagcaaa ttcctcaaac gtgcggcagg cacaaaacct gccgcgtcgg
    19981 gcatcagaag atggtgaatg gtgcgattac cataaatttc acgtcacgct catcctggaa
    20041 gatgttgccg taaccaccgc cccagcttgg gatgtcggag tggttgtcgt attcggtgaa
    20101 gtgcagtttg aacatcgtgc ctttggcgcg accgtcctga atggtgtaga ccgcatccag
    20161 gctgtatgca gactcttcaa tagtccggtt tttgtcgtag tacgcatccg gattgctctg
    20221 ccaggtcgca ggtttagcat cccatgcgta aacgtaggaa gcgccgatgg cgaagcctag
    20281 aagattccag tttttcaggt catacatcgc accgaagaag accgcttttt cgccgttagc
    20341 gttgaagtcg gaacggttat cccaccagat atccaggcgg ccgttggagg aagcgtaggc
    20401 tggagtcata cgttgcagga agtatccctg ctgaccgtca gccttaaccc aggtgccttc
    20461 gaggcgcaaa tccactacgt cagccgcccg gtaaccaaag gtcaacgctt gcagccaggc
    20521 ggtgccgtca taaaggtcgt tgacgctgcg atcgtcaact ttatcgcgcg taccatagaa
    20581 ctggtagctg gtggttaacg ggctaccggc gatatcaaat ttgtagctgg ctttggcaaa
    20641 atattgatcg atataccctt ccgcctgacc aaatgccgct tccagtacga agttattttt
    20701 gaagtcgtat ttcgccccaa gggagtgcag ataatcaact ttggtggttt tatcgttctg
    20761 ataaaactca tccatttcca gatgccacgg cgctttgtat tcgttggtcc acatgtagga
    20821 gaaactcaac gcaccagcat cgccgtaatc aaaattcgcc ccggcttccg ccccctgata
    20881 agtacctggc ataaagctcc agtgcggcgc taacagcgtt tgaccagttg gctgaatgta
    20941 acctgccctc gcccaaaccg gaccgtattt aaatttggcc gcagctttat acaggcttat
    21001 accgcttttg tcgccggacc agtcttcgtc ataggcttta ttactttttg aaaacgcgat
    21061 ttcgttcggg tgggagctgt cgccgttttc cgccatttca atcgccgtaa acacagcaat
    21121 atcaaggccg aacatatcag cagcataacc ggactgaaaa tcgaggttgg cattccaggt
    21181 ggagtgagaa aggttggttt tgtatttgtc gccgtcggta acatctttac ggtcgcgttc
    21241 acgctgccag taatatatac cgccggttaa ggttgaatcg tcgatgaatc cttcggcgcg
    21301 agcctccggc attgccataa agcccgacat tgctgtaaca ccggcgatag ccagcgccag
    21361 cgtactacgt ttgccactaa acgtacgcat gggttaatcc tctttgacgt ataaattgct
    21421 gcaccaaagg tgaacagcga aaaaaataaa aattaaaaaa gtagcattga gaaaagtctc
    21481 gctgactaca ggaatagact atttttcgcg acgcgaatta tcaatctctt tctgtaacca
    21541 gaatgtaaga acatgagcaa ccgcacatat tttgatgatt tttgttacaa ggcttaagtt
    21601 tgcggttgaa aggcttgcag aagaataaaa gcgtaaattt ccagcaagat gaaagcggtt
    21661 acattttatt taattatggt ttgtgatttt aaatttattt tatggataaa ttgcgaagcg
    21721 gaattaattc gcgaagaaaa gcagaaaaaa cgccgcagag tgcggcgttt gggattgttt
    21781 gctatttctt gccggatgcg acgtaaatgc ctcacccggc ctggtagaac ttaaaattac
    21841 tcgcctactt tcgcccaggt atcacgcagc ccaacggtgc ggttaaatac cggtttttcc
    21901 gctgtggaat ggcggctgtc gaggcagaag taaccttcac gctcaaactg gaatgcttta
    21961 cccgcaaccg catctttcag cgacggttca gcaaagccct gtttgatcac cagcgattcc
    22021 gggttaatca ccgacaggaa atcatccgca gcacctgggt taggcacgct gaacagacgg
    22081 tcgtacagac ggatttcaac cggcagcgca tgtgccgcgc tcacccagtg aataacacct
    22141 ttgactttac gaccatctgc cggatctttg cttaaggtgt cggcgtcata agtacagaag
    22201 atggtggtga tattgccttc ggcatctttc tccacgcgtt ctgccttaat gacgtaagca
    22261 ttacgcagac gcacttcttt acccagcacc agacgtttgt actgcttgtt agcttcttcg
    22321 cggaaatcgg cgcgatcgat ccaaatctca ctgctaaacg gcacctggcg gctacccatt
    22381 tccggtttgt tcggatggtt cggcatggta accatttcgc cttcgccctg atagttttcg
    22441 ataaccagtt tcaccggatc gataaccgcc attgcgcgcg gggcgttttc gttgagatct
    22501 tcacggatgc aggattccag cgacgccatc tcaatggtgt tgtcctgcct ggtcacgccg
    22561 atgcgtttgc agaactcacg aatagaagca gcagtgtaac cacgacgacg cagaccggaa
    22621 atggtcggca tacgcgggtc atcccagcct tcaacgtgct tgtcggtcac cagcaggttc
    22681 agcttacgct tggacatcac agtgtattcc agattcaggc gcgagaattc gtactggcgc
    22741 gggtgaacag gaatggtgat gttgtccagc acccagtcgt acaggcgacg gttgtcctgg
    22801 aactcaagcg tacacagaga gtgcgtaata ccttccaggg catcgctgat gcagtgggtg
    22861 aagtcgtaca tcgggtagat gcaccacttg ttgccagtct ggtggtgttc agcaaattta
    22921 atgcggtaca gcaccggatc gcgcatcacg ataaacggtg aagccatgtc gattttcgca
    22981 cgcaggcagg ctttaccttc ttcaaaacca ccggcacgca ttttttcgaa cagcgccagg
    23041 ttctcttcaa cgctgcggtc gcggtacggg ctgtttttac ccggttgcgt cagggtgccg
    23101 cggtattcgc ggatctgttc cggcgtcagt tcatcaacgt acgccaggcc tttattgatc
    23161 agttcgatcg cataggcgtg gagctgatca aaataatcgg aggagtaacg gacgttacca
    23221 gaccagtgaa aacctaacca ctctacgtcg tttttgatcg actcaacata ctcgatatct
    23281 tcttttaccg ggttagtgtc gtcgaaacgc aggttgcact ggcctttata gtcctgggcg
    23341 atcccgaagt tcaggcagat agatttcgca tggccaatat gcagatagcc attcggctcc
    23401 ggcgggaaac gggtgtgtac tgtggtgtgc ttaccactgg ccagatcttc atcgatgatc
    23461 tgacggataa agttagtcgg gcgggcttct gcctcactca tcgtggattc ctcaaagcgt
    23521 aaacaacgta taacggcgta tgatcttata agcggaacag gctgacaact gttagttttt
    23581 taaaattgat aatctaacaa ggattatggg gatgattgat gaaaaaaaaa gcggcagaga
    23641 agatctccac cgcttgtcat tgttggatgc gacgctcaag cgtcgcatca ggcataaagc
    23701 agattacttt ttgatttcat acagcggtgt ttgacccgcc acaacatggc cctgagcttt
    23761 aatgatcaag ccactgaaat cgtcgatatt gctgcaaacc accgggctaa tcatcgagcg
    23821 ggcgttagcg ttcaggtaat ccagatccat ttccagaatc ggttgccctg cgcttacctg
    23881 cgcaccctct tccaccagac gtttaaagcc tttaccttcc agcgctacgg tgtcgatacc
    23941 catatggacg acgatctccg cgcctttttc ggtttccagg cagaacgcgt ggttggtgtt
    24001 gaagattttc acgattgtcc ctgcggctgg tgatacgacg attttatctg tcggtttcac
    24061 cgccacaccg tcacccaccg ctttgctggc gaatgcttcg tcaggaacct gatccagtgc
    24121 cacgacatca ccggtaatcg gcgataccag ctccgcgata gataccgcgt ttggtacagc
    24181 ctgcggtttt gctacaggcg cggcagttga cggagttgct tcagctgacg cagcggctac
    24241 cggaccacgg gcaacgactt tcttcatcgc atcgccgatg gattctgctt tcgcgccaac
    24301 aatcacctga atagtctgtt tgttcagttt cactacacca gaagcaccca gacgtttaca
    24361 catcgtatcg ttgacgcggg cagagtcagc cacggtcagg cgcagacggg tgatacaggc
    24421 gtcaattgct ttcaggttgt cagtgccgcc aaccgcagca atatagttgg tcgccagttg
    24481 attgaggcct tcttcagtgt tgctgttggc ttcttcagta acgatctcat cttctttatc
    24541 ttcacgaccc ggcgttttca ggttgaacat gcggataacc aaactgaaca ccacgaagta
    24601 gatagcgaag aagacaacac ccatcaccag cagcatccag acgttctggc tggcggccgg
    24661 caggttatac atcaacgcgt agtcgatagc ccccgcagag aaagagaagc cagcatggat
    24721 acccagcagt gttgccacaa acaggctgat accggtcagc agtgcgtgca ggaggtacag
    24781 cagcggtgca aggaacatga acaggaattc cagcggctca gtcacaccgg tcaggaacgc
    24841 agtaacagca acagaaagca gcatcccgcc aaccatcgga cgacgctctt tcggtgctgc
    24901 gaagtacatc gccagcgccg cacccggcag accgaacatc ataatcggga agaagccgga
    24961 catgaacatc cccgcggtgc cgtcaccggc atagaagcgg ttgatatcac cgtggaaaac
    25021 cgtacccgcc gcgttggtga attcaccaat ctggaaccag gcgatggtgt tcagcacctg
    25081 atgcagaccg gttgggatca gcagacggtt gatgaaacca aagataccgg aacccagcgc
    25141 gcccgcagaa acgatccact cgcctcctgc atggatagcg tgctgtaccg gcggccagac
    25201 gtaaccaaaa atggccgcca gcaccaggca gaagaagccg gtggcaatcg gcacaaagcg
    25261 tttgccgccg aagaagctca ggaagtccgg cagtttaata tcggaccaac ggttataggc
    25321 tgcgccacca accagaccgg taatgatacc cgccagtaca cccatgttta tttctgggtt
    25381 gatggtcacc atcgctttgg ttaacacaaa gtaacctacc gcacccgcca gcgccgccgc
    25441 acctgcgctg tctttcgacc agctggatgc cacaccgatg gcgaagatta atgcgaggtt
    25501 atcaaaaatc gcaccgcccg cctgggcaat aaacgcaacg ttaagtaaat ctggctgacc
    25561 gaatcgcagc aacagtgccg ccaccggcag caccgcgata gggagctgta acgccctacc
    25621 gagtcgctgg aaaaaaccta aaatattcat cttattcccc ctacgagaac cctatttggc
    25681 tcgtttaaag ccgtattttt attttgctgc aaattgtact gccgatgttc tgtaatcaga
    25741 ttgttagatc atctgctaca gagtgtgtga aaatttaatt cgtatcgcaa attaaacgcg
    25801 tgtcttttgt gagttttgtc accaaatatc gttatcatca ctccctttta ctggctaaac
    25861 cagaaaactt attttatcat tcaaaaaatc aggtcggatt gacgcctgtc tgcgcaaatc
    25921 caagttacgc ttaaagatgc ctaatccgcc aacggcttac attttactta ttgaggtgaa
    25981 taatgagact gatccccctg actaccgctg aacaggtcgg caaatgggct gctcgccata
    26041 tcgtcaatcg tatcaatgcg ttcaaaccga ctgccgatcg tccgtttgtt ctgggcctgc
    26101 caactggcgg cacgccgatg accacctata aagcgttagt cgaaatgcat aaagcaggcc
    26161 aggtcagctt taagcacgtt gtcaccttca acatggacga atatgtcggt ctgccgaaag
    26221 agcatccgga aagctactac agctttatgc accgtaattt cttcgatcac gttgatattc
    26281 cagcagaaaa catcaacctt ctcaacggca acgccccgga tatcgacgcc gaatgccgcc
    26341 agtatgaaga aaaaatccgt tcttacggaa aaattcatct gtttatgggc ggtgtaggta
    26401 acgacggcca tattgcattt aacgaaccgg cgtcttctct ggcttctcgt actcgtatca
    26461 aaaccctgac tcatgacact cgcgtcgcga actctcgttt ctttgataac gatgtcaatc
    26521 aggtgccaaa atatgccctg actgtcggtg ttggtacatt gctggatgcc gaagaagtga
    26581 tgattctggt gctgggtagc cagaaagcac tggcactgca ggctgcagtt gaaggttgcg
    26641 tgaaccacat gtggaccatc agctgtctgc aactgcatcc aaaagcgatc atggtgtgcg
    26701 atgaaccttc caccatggag ctgaaagtta agactttaag atatttcaat gaattagaag
    26761 cagaaaatat caaaggtctg taattgttat ccctgccctg gctccttgct cagggcaata
    26821 ttttttaaaa tcgggggtca gaatgtatgc attaacccag ggccggatct ttaccggcca
    26881 cgaatttctt gatgaccacg cggttgttat cgctgatggc ctgattaaaa gcgtctgtcc
    26941 ggtagcggaa ctgccgccag agatcgaaca acgttcactg aacggggcca ttctctcccc
    27001 cggttttatc gatgtgcagt taaacggctg cggcggcgta cagtttaacg acaccgctga
    27061 agcggtcagc gtggaaacgc tggaaatcat gcagaaagcc aatgagaaat caggctgtac
    27121 taactatctg ccgacgctta tcaccaccag cgatgagctg atgaaacagg gcgtgcgcgt
    27181 tatgcgcgag tacctggcaa gacatccgaa tcaggcgtta ggtctgcatc tggaaggtcc
    27241 gtggctgaat ctggtaaaaa aaggcaccca taatccgaat tttgtgcgta agcctgatgc
    27301 cgcgctggtc gatttcctgt gtgaaaacgc cgacgtcatt accaaagtga ccctggcacc
    27361 ggaaatggtt cctgcggaag tcatcagcaa actggcaaat gccgggattg tggtttctgc
    27421 cggtcactcc aacgcgacgt tgaaagaagc gaaagccggt ttccgcgcgg ggattacctt
    27481 tgccacccat ctgtacaacg cgatgccgta tattaccggt cgtgaaccgg gcctggcggg
    27541 cgcgatcctc gacgaagctg acatttattg cggtattatc gctgatggcc tgcatgttga
    27601 ttacgccaac attcgtaacg ctaaacgcct taaaggcgac aaactgtgtc tggttaccga
    27661 cgccaccgcg ccagcaggtg ccaacattga acagttcatt tttgcgggta aaacaatata
    27721 ctaccgtaac ggactttgtg tggatgagaa cggtacgtta agcggttcat ccttaaccat
    27781 gattgaaggc gtgcgtaatc tggtcgaaca ttgcggtatc gcactggatg aagtgctgcg
    27841 tatggcgacg ctctatccgg cgcgtgcgat tggcgttgag aaacgtctcg gcacactcgc
    27901 cgcaggtaaa gtagccaacc tgaccgcatt cacacctgat tttaaaatca ccaagaccat
    27961 cgttaacggt aacgaggtcg taactcaata agagaaagta tgacaccagg cggacaagct
    28021 cagataggta atgttgatct cgtaaaacag cttaacagcg cggcggttta tcgcctgatt
    28081 gaccagtacg ggccaatctc gcggattcag attgccgagc aaagccagct tgcccccgcc
    28141 agcgtaacca aaattacgcg tcagcttatc gaacgcgggc tgatcaaaga agttgatcag
    28201 caggcctcca ccgggggccg ccgcgctatc tccatcgtca ccgaaacccg caatttccac
    28261 gcaatcggcg tacggcttgg tcgtcacgac gccaccatca ccctgtttga tctcagcagc
    28321 aaagtgctgg cagaagaaca ttacccgctg ccggaacgta cccagcaaac gctggaacat
    28381 gccctgttga atgccattgc tcagtttatt gatagctacc agcgcaaact gcgcgagctg
    28441 atcgcgattt cggtgatcct gccagggctt gttgacccgg acagcggcaa aattcattac
    28501 atgccgcata ttcaggtaga aaactggggg ctggtagaag ctctggaaga acgttttaaa
    28561 gtgacctgtt tcgttggtca cgatatccgt agtctggcgc tggcggagca ctacttcggt
    28621 gcaagtcagg attgcgaaga ctccattctg gtgcgtgtcc atcgcggaac cggggccggg
    28681 attatctcta acgggcgcat ttttattggc cgcaacggta acgtcggtga aattggccat
    28741 attcaggtcg aaccgctggg tgaacgctgc cactgcggca actttggctg cctggaaact
    28801 atcgccgcca acgctgccat tgaacaacgg gtgttgaatc tgttaaagca gggctaccag
    28861 agccgcgtgc cgctggacga ctgcaccatc aaaactatct gcaaagccgc gaacaaaggc
    28921 gatagtctgg cgtcggaagt aattgagtat gtcggtcgtc atctgggtaa aaccatcgcc
    28981 attgctatca acttatttaa tccgcaaaaa attgttattg ccggtgaaat caccgaagcc
    29041 gataaagtgc tgctccctgc tattgaaagc tgcattaata cccaggcgct gaaggcgttt
    29101 cgcactaatc tgccggtggt acgttctgag ctggatcacc gctcggcaat cggcgctttt
    29161 gcgctggtaa aacgcgccat gctcaacggt attttgctcc agcatttgct ggaaaattaa
    29221 tgtgctttta tagtggcgct tattgttgtc aatattctgg gtagtccatg accattaaaa
    29281 atgtaatttg cgatatcgat ggcgtgctga tgcacgataa cgtcgccgta ccgggtgcag
    29341 cggaattttt gcacgggatt atggataaag gcctgccgct ggtgttgctg accaactatc
    29401 cttcgcagac tgggcaagat ctggcgaacc gctttgccac cgcaggtgtc gatgtacctg
    29461 acagcgtgtt ttatacctct gcgatggcga ctgccgattt cctgcgtcgc caggaaggca
    29521 agaaagcgta tgtggtgggc gaaggcgcac tgattcatga actgtacaaa gccggtttca
    29581 ctattaccga tgtgaaccct gatttcgtga ttgttggcga aacgagttcc tacaactggg
    29641 acatgatgca taaagcagcc tatttcgtcg ctaacggtgc acgttttatc gccaccaatc
    29701 cggacaccca cgggcgcggt ttttatcccg cgtgtggcgc gttgtgtgca ggcatcgaaa
    29761 aaatctccgg gcgtaaaccg ttctatgttg gtaaaccgag tccgtggatc atccgcgcgg
    29821 cattaaacaa aatgcaggcg cattcggaag aaacggtgat tgtcggcgat aacctgcgta
    29881 ccgatattct ggcgggcttc caggcaggtc tggaaacgat tctggtgctt tctggtgttt
    29941 catcgctcga cgatatcgac agtatgccgt tccgcccaag ctggatttac ccgtcggtcg
    30001 ctgaaatcga cgttatctga atagcaacca cgtctgcgga cgtggttggc tctgatgaca
    30061 aacgccaaac tgcctgatgc gctacgctta tcaggcctac gcagctcctg caatatattg
    30121 aattttcatg cttttgtagg ccggataagg cgttcacgcc gcatccggca tgaacaaatt
    30181 gcactttgtc agcaatttgg cccccgtaaa atacaaacat tcaataaaac ccgctgaaaa
    30241 ttatcgattc atcaataaat atatcctcac accagacctt ttttcaccaa tcgacaatca
    30301 ccattacgtt ttttattttt tcaccgccaa atcgcttgcg cacagtgctg ctttgcggca
    30361 tttttttaaa caagcaaaca caacaagcaa caaataccag attaacggag aaggttatgt
    30421 gttcaatttt tggcgtattc gatatcaaaa cagacgcagt tgagctgcgt aagaaagcac
    30481 tcgagctgtc acgcctgatg cgtcatcgtg gcccggactg gtccggtatt tatgccagcg
    30541 ataacgccat tctcgctcac gaacgtctgt caattgttga cgttaacgcg ggggcgcaac
    30601 ctctctacaa ccaacaaaaa acccacgtac tggcggtaaa cggtgaaatc tacaaccacc
    30661 aggcattgcg cgctgaatat ggcgatcgtt atcagttcca gacagggtct gactgtgaag
    30721 tgatcctcgc gctgtatcag gaaaaagggc cggaatttct cgacgacttg cagggcatgt
    30781 ttgcctttgc cctgtacgac agcgaaaaag atgcctacct gattggtcgc gaccatctgg
    30841 ggatcatccc actgtatatg ggctatgacg aacacggtca gctgtatgtg gcctcagaaa
    30901 tgaaatcgct ggtgccagtt tgccgcacga ttaaagagtt cccggcgggg agctatttgt
    30961 ggagccagga cggcgaaatc cgttcttatt atcatcgcga ctggttcgac tttgatgcag
    31021 tgaaagataa cgtgaccgac aaaaacgagc tgcgtcaggc tctggaagat tccgttaaaa
    31081 gccatctgat gtctgatgtg ccttacggtg tgctgctttc tggtggtctg gattcctcaa
    31141 ttatttccgc tatcaccaag aaatacgcag cccgtcgcgt ggaagatcag gaacgctctg
    31201 aagcctggtg gccgcagtta cactcctttg ctgtaggtct gccgggttca ccggatctta
    31261 aagcagccca ggaagtggca aaccatctgg gcacggtgca tcacgaaatt cacttcactg
    31321 tacaggaagg tctggatgcc atccgcgacg tgatttacca catcgaaact tatgatgtga
    31381 ccactattcg cgcttcaaca ccgatgtatt taatgtcgcg taagatcaag gcgatgggca
    31441 ttaaaatggt gctgtccggt gaaggttctg atgaagtgtt tggcggttat ctttacttcc
    31501 ataaagcgcc caacgctaaa gaactgcatg aagagacggt gcgtaaactg ctggccctgc
    31561 atatgtatga ctgcgcgcgc gccaacaaag cgatgtcagc ctggggcgtg gaagcacgcg
    31621 ttccgttcct cgacaaaaaa ttccttgatg tggcgatgcg cattaacccg caggataaaa
    31681 tgtgcggtaa cggcaaaatg gaaaaacaca tcctgcgtga atgttttgag tcatacctgc
    31741 ccgcaagcgt ggcctggcgg cagaaagagc agttctccga tggcgtcggt tacagttgga
    31801 tcgacaccct gaaagaagtg gcggcgcagc aggtttctaa tcagcaactg gaaactgccc
    31861 gcttccgctt cccgtacaac acgccaacct caaaagaagc gtatctgtac cgggagatct
    31921 ttgaagaact gttcccgctt ccgagcgccg ctgagtgcgt gcctggcggt ccttccgtcg
    31981 cgtgttcttc cgctaaagcg atcgaatggg atgaagcgtt caagaaaatg gacgatccat
    32041 ctggtcgtgc ggttggtgtt caccagtcgg catataagta agaaatgcga aacagccccg
    32101 gtgaaatccg gggctgtttc agttattgag gtgcttgttg attctgatat gcagtaagga
    32161 aatgttccgg gtttgccaca tcactgtttg acaactcatc actgtaggcc agtatttcat
    32221 agattaattt cccaccacta ttgttgaatt ccgaatttca tcctgtacga caaaagaatg
    32281 gctgtagggt ggggaaaaaa cacaaagtaa acaataattg acgaatatag cgccacgctg
    32341 ttcgcaacct aaccaaatag tcactttcga gcaattttcc ttgaaaaaga ggttgacgct
    32401 gcaaggctct atacgcataa tgcgccccgc aacgccgata aggtatcgcg aaaaaaaaga
    32461 tggctacgta gctcagttgg ttagagcaca tcactcataa tgatggggtc acaggttcga
    32521 atcccgtcgt agccaccatc tttttggcgg gagtggcgaa attggtagac gcaccagatt
    32581 taggttctgg cgccgcaagg tgtgcgagtt caagtctcgc ctcccgcacc attcaccaga
    32641 aagtgttgta cggatggggt atcgccaagc ggtaaggcac cggtttttga taccggcatt
    32701 ccctggttcg aatccaggta ccccagccat cttcttcgag taagcggttc accgcccggt
    32761 tattggggta tcgccaagcg gtaaggcacc ggtttttgat accggcattc cctggttcga
    32821 atccaggtac cccagccata gaagaaacaa tctggctacg tagctcagtt ggttagagca
    32881 catcactcat aatgatgggg tcacaggttc gaatcccgtc gtagccacca aattctgaat
    32941 gtgtcgaata cgttcggcaa attcaaaaac caatttgttg gggtatcgcc aagcggtaag
    33001 gcaccggatt ctgattccgg cattccgagg ttcgaaccct cgtaccccag ccaatttatt
    33061 caagacgctt acatcgtaag tgcacccagt tggggtatcg ccaagcggta aggcaccgga
    33121 ttctgattcc ggcattccga ggttcgaatc ctcgtacccc agccacatta aaaaagctcg
    33181 cttcggcgag ctttttgctt ttctgcgttc attcaatgtc gaatgcgatg ttgacacgtc
    33241 ttatccttca atgtcggatg cgacgctgcc gcgtcttatc cgacctacgg ttggcacgca
    33301 tccggcaatg ttgtaaggct acaaccctaa cgcatatttc agcgcctgac gtttcaacac
    33361 gccagcacgc tctgccgcca ttaacccgag attacgcaca aaacgcagcg gcggcagatt
    33421 attactgaat ccggcataaa acagatccat accactttgc ataatgaagt tatccgccat
    33481 gcgccgcatc tggtaacgct tgaggacagg ataactggcc cacgcttcac cgtaactgcg
    33541 ggcattcacc agaacatcaa tcagggcatc gacatcacga taaccaagat tcaccccctg
    33601 ccccgccagc ggatggatgg tatgcgcggc atcgcccacc agcgcaagcc ccggctgcac
    33661 atactgcaac gcatgtcggc gcgtcagcgg aaacgcacca gcggcaagcg gtgtaacgta
    33721 acccagacgc gacgggaaat gcttcgcgat ttccgcctgg agttgcgcca tattcatatt
    33781 ctgcaactgg cgaatacgcg ccggagagtc ataccacacc agcgatgccc agttatcaaa
    33841 caacggcaga aacgcacgcg gtccgtccgg ggtaaattgc tgccaggtgc tgtcgccggg
    33901 atcgttctca cactgtacgc taatcaacat gcacgactgc gcatactgcc aggcatgaac
    33961 gccaattccc gccatctgcc gcacctgaga atttgcgccg tcggcaccaa tcaccagttt
    34021 cgcgcggatc gtttcaccgc ccttcagttc cagctcctga agatcattat ctcgatgtaa
    34081 tgcaatcagc gagcctggca cacgtaacgt tactttcgga tgcgcttcca gcgcctgcca
    34141 cagcgcctgt tgcagtacgg tgttttccac catatagcca agcaacggta gcttaagttc
    34201 agcggcgtca aacaccacat gcgccgtttc ccactcccac gtttccagtc tgcggtaagg
    34261 atggcaacgc atagcctgta ctgcatccca gacccctaac cctttaagca atgataccga
    34321 tgcggcgctg atcgccgaga tccgcacgtc cggttggcta tcagcgacaa acggtgcagg
    34381 ttctgcgtgc tcgatcaccg ttaccgaaaa tccgtgctgt gccagcccca gcgccagcgc
    34441 gccgccgacc attcctccgc cgacaatggc aatttccgtt ggttgatttg tcatggtcaa
    34501 tcatcctgtt agcaatatgc tttaagttta caggattttt acccccaagg gctgataacg
    34561 ctcatactgg ccacaacggc agcaaagcat tacactatgc gccctgcatt cctggctact
    34621 atttcgcaag agcaagtcga tgaccaaaaa actccatatt aaaacctggg gctgtcagat
    34681 gaacgagtac gattcatcga agatggccga tctgctggat gccacccacg gctatcaact
    34741 gaccgacgtg gcggaagaag cggatgtgct gctgctgaac acctgctcaa tccgcgagaa
    34801 ggctcaggaa aaagtcttcc atcagttggg tcgctggaaa ctgttaaaag agaagaatcc
    34861 agacctgatt atcggcgtcg gtggctgcgt ggcatcgcaa gaaggcgagc acattcgcca
    34921 gcgcgcccac tatgtcgata ttatttttgg gccgcaaacg ctgcaccgtc tgccggagat
    34981 gatcaactcc gtgcgcggcg accgcagccc ggttgtagat atcagcttcc cggaaatcga
    35041 gaagtttgac cgtctaccgg aaccgcgcgc cgaagggccg accgcgtttg tctccatcat
    35101 ggaaggctgc aataaatatt gcacctactg cgtggtgcct tacacccgtg gtgaagaggt
    35161 aagccgtccg tctgacgata ttctgtttga gattgcccag cttgcggctc agggcgtgcg
    35221 tgaagtcaac ctgctcggtc agaacgtgaa cgcctggcgt ggtgaaaact acgacggctc
    35281 caccggatcg tttgccgatc tgctgcgtct ggttgctgcg atcgacggga tcgatcgtat
    35341 tcgctttacc accagccatc cgatcgaatt caccgacgat atcatcgaag tgtatcgcga
    35401 cacgccggag ctggtgagct tcctgcatct gccggtacag agcggttccg atcgcattct
    35461 gaacctgatg ggccgtactc atacggcact ggagtacaaa gcgatcatcc gtaaactgcg
    35521 tgcggcgcgt ccagatattc agatcagttc tgatttcatc gttggcttcc ctggcgaaac
    35581 caccgaagac ttcgagaaaa cgatgaagct gattgccgac gtcaatttcg acatgagcta
    35641 cagctttatc ttctctgcac gtccgggtac accagccgcc gatatggttg atgatgttcc
    35701 ggaagaagag aagaagcagc gtctgtatat tctgcaagaa cgtatcaatc agcaagcgat
    35761 ggcatggagc cgccggatgc tcggcaccac ccagcgtatt ctggtagaag ggacttcgcg
    35821 taagagcatc atggagcttt ccgggcgtac cgaaaataac cgcgtggtca acttcgaggg
    35881 cacaccggat atgatcggta aattcgtcga tgtagaaatt accgacgtct acccgaactc
    35941 tctgcgcggt aaagtggtgc gtactgaaga tgaaatgggc ctgcgcgtgg cagaaacacc
    36001 ggaatcggtg attgcccgta cccgcaaaga aaacgacctt ggcgtgggtt attatcagcc
    36061 gtaattctca ggcccgccgt tccggtgggc cttgtttttt ctctctttat ccccatcttt
    36121 atcgcaatgc ttgcgccttt ttcctgtggc gtgaataatt tccttatagg ccggtgaatg
    36181 ttcagcttcc ggcggcatac agataagagg aacggtttga acatagacac tcgcgaaatc
    36241 accctggagc cagcagacaa cgcgcgtctg ttgagcctgt gcggcccgtt tgatgacaac
    36301 atcaagcagc tcgaacgccg tctcggcatc gagatcaatc gccgcgacaa ccactttaaa
    36361 ctgaccggcc gtccgatttg cgtcaccgct gcggcagaca ttctgcgtag cctgtatgtc
    36421 gatacagccc cgatgcgcgg tcagattcag gatatcgaac cggaacagat ccaccttacg
    36481 attaaagaag cgcgagtact ggagcaaagt gcggagagcg tgccggagta cggcaaagcg
    36541 gtcaatatca aaaccaaacg cggcgtaatt aagccgcgca cgccaaacca ggcgcagtac
    36601 atcgccaata ttctcgacca tgacattacc ttcggcgttg gcccggcggg tacgggtaaa
    36661 acctacctgg cagtggctgc ggcagttgat gccctggagc gtcaggagat tcgccgtatt
    36721 ctgctgactc gtccggcggt cgaagccggt gagaaactgg gcttcctgcc tggcgattta
    36781 agccagaaag tagacccgta tctgcgccca ctgtacgacg cgctgtttga aatgctgggc
    36841 tttgagaaag ttgagaaact gattgagcgc aacgttattg aagtcgcgcc gctggcctat
    36901 atgcgtggtc gtacgctgaa cgacgcgttt atcattctcg atgagagcca gaacaccacc
    36961 atcgaacaga tgaagatgtt cctgacccgt atcggtttta actcaaaagc ggttatcacc
    37021 ggcgacgtca cacagatcga cctgccgcgt aatactaaat caggcttacg tcacgccatc
    37081 gaagtactgg ccgatgtcga agagatcagc tttaacttct tccacagcga agacgtggtt
    37141 cgtcaccctg tggtggcgcg tatcgttaac gcctatgaag cctgggaaga agccgaacaa
    37201 aaacgaaaag cggcgctggc ggcagaacgc aagcgcgaag aacaggaaca aaaatgagtc
    37261 aggtgatcct cgatttacaa ctggcatgtg aagataattc cgggttaccg gaagagagcc
    37321 agtttcaaac atggctgaat gcggtgatcc cgcagtttca ggaagaatcg gaagtgacta
    37381 ttcgcgtggt cgataccgcc gaaagccaca gtctgaatct gacctatcgc ggtaaggata
    37441 agccgaccaa cgtgctctcc ttcccgtttg aagtgccgcc tggcatggaa atgtcgctac
    37501 tgggcgatct ggttatctgc cgtcaggtgg ttgagaagga agctcaggag caaggcaaac
    37561 cactggaggc gcactgggcg catatggtgg tgcacggcag tctgcatttg ttaggttacg
    37621 atcacatcga agatgacgaa gcagaagaaa tggaagccct cgaaacagag attatgcttg
    37681 ctctgggcta tgaggatccg tacattgccg agaaagaata atttgccagg catttgactg
    37741 gcagacgtcc ccgccgttgg tgattaacga cggcgttttg attaactaac atgagaaccc
    37801 atacgacgcc atgagcgacg acaattcaca cagtagtgac acgataagca acaagaaggg
    37861 atttttctcc ctgttactca gccaactttt ccacggtgaa ccgaaaaacc gtgacgaact
    37921 gctggcgctg atccgtgatt ccgggcagaa cgaccttatc gacgaagata cgcgcgatat
    37981 gctcgaaggg gtgatggaca tcgcagacca acgcgttcgc gacatcatga tcccccgctc
    38041 ccagatgatt accctgaaac gcaaccagac gctggacgaa tgccttgatg tcatcatcga
    38101 gtccgcccac tcacgtttcc cggtgattag cgaagacaaa gatcacattg aagggattct
    38161 gatggcgaaa gacttgctgc cgtttatgcg cagcgatgct gaagccttca gcatggacaa
    38221 agtgttacgt caggcggttg tcgttcctga aagtaagcgc gtagaccgga tgctgaaaga
    38281 gtttcgctct cagcgttacc acatggcgat cgtcattgac gaattcggtg gggtttccgg
    38341 tctggtgacc attgaagaca tcctggaact gattgttggt gagattgaag acgaatatga
    38401 cgaagaagat gatatcgact tccgtcagct gagtcgtcat acctggaccg tgcgcgcact
    38461 ggcttccatt gaagacttca acgaagcgtt cggcacccac tttagcgatg aagaagtcga
    38521 cactatcggt ggtctggtga tgcaggcgtt tgggcatctt ccggcgcgtg gcgaaactat
    38581 cgacatcgac ggttaccagt tcaaagtggc gatggccgac agtcgacgta ttattcaggt
    38641 tcatgtcaaa atcccggatg actcacccca gccgaagctg gatgaataaa accgaaactg
    38701 gatagataac tacatggctt ttgcctcatt aattgaacgc cagcgcattc gcctgctgct
    38761 ggcgttatta ttcggtgcct gcggaacgct ggccttctct ccttacgacg tctggcctgc
    38821 ggcgattatt tcgttgatgg ggcttcaggc gttgaccttt aaccgccgtc cactccagtc
    38881 tgccgctatt ggcttttgct ggggatttgg cctctttggc agcggtatta actgggtcta
    38941 tgtcagcatc gcgacctttg gcggaatgcc tggcccggtt aacatcttcc tggtggtgct
    39001 gctggcggcg tatttgtcgc tgtataccgg actgtttgct ggcgtgctgt cgcgtctgtg
    39061 gccgaaaacc acctggctgc gcgtggcgat tgccgcccct gctctctggc aagtgaccga
    39121 gtttctgcgc ggttgggtac tgacaggctt cccgtggtta cagttcggct atagccagat
    39181 tgatggcccg ttaaaagggc tggcaccgat aatgggcgtg gaagccatta acttcctgct
    39241 gatgatggtt agtggcctgc tggcactggc gttggtcaaa cgcaactggc gtccgctggt
    39301 ggtggccgtc gtgctgtttg ccctaccctt cccgctgcgt tacatccaat ggtttacccc
    39361 gcaaccggag aaaaccattc aggtttcgat ggttcagggc gatattccgc aatcactgaa
    39421 atgggatgga gatcagcttc ttaatacgct gaagatttac tacaacgcaa cggctccgct
    39481 gatgggcaaa tcatcgttga ttatctggcc ggagtcggcg ataaccgatc tggaaattaa
    39541 tcagcaaccg ttcctcaaag cactggacgg tgagttgcgc gataaaggta gctcgctggt
    39601 aaccgggatt gtcgacgcgc gtctcaataa gcagaaccgc tacgatacct acaacaccat
    39661 catcacactg ggtaaaggtg caccgtacag ctacgaatca gccgatcgct ataacaaaaa
    39721 ccatctggtg ccgtttggcg agtttgtccc gctggagtcg attctgcgtc cgttagcacc
    39781 gttctttgat ctgccgatgt cgtcgttcag ccgtgggcca tatatccagc cgccgctgtc
    39841 agtaaatggt attgagctta ctgcggctat ttgctacgag atcattctcg gcgagcaagt
    39901 gcgcgataac ttccgcccgg ataccgacta tctgctgact atctccaacg atgcgtggtt
    39961 tggtaaatct attggtccat ggcaacactt ccagatggcg cgaatgcgtg cgctggagct
    40021 ggcgcgccca ctgttgcgca gcaccaacaa cggcattacg gcggtgattg gcccgcaggg
    40081 tgagattcag gcgatgatcc cgcagttcac ccgcgaggtg ttaaccacta atgtgacacc
    40141 gaccaccgga ctcacaccgt atgcacgtac cggcaactgg ccactgtggg tgctgacggc
    40201 actgtttggt tttgccgctg tgttgatgag tctgcgagca cgtaaacgtt gatgtgcctg
    40261 atgtatcctg catgagcaaa acgtactttc tcattgagct ttggggcatg gttatcatgc
    40321 ccctttttct tacggttcag ggatacaagc cacattgcca tattggggat gacgaataat
    40381 tgcctgcgga tcaagcgagc gcgtcctgtc catttggatc aacccgttct tcgccatgac
    40441 aaatattttt ctaccatccg ctgaa
//
LOCUS       DAJVUJ010000013        40426 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.013, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000013 DAJVUJ010000000
VERSION     DAJVUJ010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40426
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.013"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(116..2437)
                     /locus_tag="OQQ48_000700"
     CDS             complement(116..2437)
                     /locus_tag="OQQ48_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCS1951563.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFITRYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            complement(2534..3010)
                     /gene="greB"
                     /locus_tag="OQQ48_000701"
     CDS             complement(2534..3010)
                     /gene="greB"
                     /locus_tag="OQQ48_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HCS1951564.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            3238..3957
                     /gene="ompR"
                     /locus_tag="OQQ48_000702"
     CDS             3238..3957
                     /gene="ompR"
                     /locus_tag="OQQ48_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HCS1951565.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            3954..5306
                     /gene="envZ"
                     /locus_tag="OQQ48_000703"
     CDS             3954..5306
                     /gene="envZ"
                     /locus_tag="OQQ48_000703"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HCS1951566.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(5382..7004)
                     /gene="pckA"
                     /locus_tag="OQQ48_000704"
     CDS             complement(5382..7004)
                     /gene="pckA"
                     /locus_tag="OQQ48_000704"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCS1951567.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            7383..9107
                     /locus_tag="OQQ48_000705"
     CDS             7383..9107
                     /locus_tag="OQQ48_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HCS1951568.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FGCYLLLMAMLLLPWIQQSLRIRNCSSRYSYFYQSVWHNVLILLVIFLANGLTWLVLL
                     LWGELFKLVGIKFFNTLFFATDWFMYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGALAVLVLLVWSLGY
                     FVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     LAENVLIAPGSVKPDVTFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            complement(9242..10120)
                     /gene="hslO"
                     /locus_tag="OQQ48_000706"
     CDS             complement(9242..10120)
                     /gene="hslO"
                     /locus_tag="OQQ48_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCS1951569.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSREHCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(10145..10546)
                     /gene="hslR"
                     /locus_tag="OQQ48_000707"
     CDS             complement(10145..10546)
                     /gene="hslR"
                     /locus_tag="OQQ48_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HCS1951570.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            complement(10557..11225)
                     /gene="yrfG"
                     /locus_tag="OQQ48_000708"
     CDS             complement(10557..11225)
                     /gene="yrfG"
                     /locus_tag="OQQ48_000708"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HCS1951571.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKTSGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            complement(11290..13425)
                     /gene="igaA"
                     /locus_tag="OQQ48_000709"
     CDS             complement(11290..13425)
                     /gene="igaA"
                     /locus_tag="OQQ48_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HCS1951572.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRSQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLDQQTDIDIYLDCHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            13745..14305
                     /gene="nudE"
                     /locus_tag="OQQ48_000710"
     CDS             13745..14305
                     /gene="nudE"
                     /locus_tag="OQQ48_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HCS1951573.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            complement(14470..17022)
                     /gene="mrcA"
                     /locus_tag="OQQ48_000711"
     CDS             complement(14470..17022)
                     /gene="mrcA"
                     /locus_tag="OQQ48_000711"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HCS1951574.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            17142..17921
                     /gene="hofM"
                     /locus_tag="OQQ48_000712"
     CDS             17142..17921
                     /gene="hofM"
                     /locus_tag="OQQ48_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HCS1951575.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLENDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALFVDPDS
                     VAICGEGGFDPWEAVSVRQPLLPPPGGDFAIALGLALGKAY"
     gene            17921..18460
                     /gene="hofN"
                     /locus_tag="OQQ48_000713"
     CDS             17921..18460
                     /gene="hofN"
                     /locus_tag="OQQ48_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HCS1951576.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLRKQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            18444..18884
                     /gene="hofO"
                     /locus_tag="OQQ48_000714"
     CDS             18444..18884
                     /gene="hofO"
                     /locus_tag="OQQ48_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HCS1951577.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWATLYRLVDTTPFSEEKTLPFSPLDFQLPGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            18874..19278
                     /gene="hofP"
                     /locus_tag="OQQ48_000715"
     CDS             18874..19278
                     /gene="hofP"
                     /locus_tag="OQQ48_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HCS1951578.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            19190..20428
                     /gene="hofQ"
                     /locus_tag="OQQ48_000716"
     CDS             19190..20428
                     /gene="hofQ"
                     /locus_tag="OQQ48_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HCS1951579.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNNNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGIEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEYERRELVVFITPRLVSSE"
     gene            20829..21350
                     /gene="aroK"
                     /locus_tag="OQQ48_000717"
     CDS             20829..21350
                     /gene="aroK"
                     /locus_tag="OQQ48_000717"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HCS1951580.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            21407..22495
                     /gene="aroB"
                     /locus_tag="OQQ48_000718"
     CDS             21407..22495
                     /gene="aroB"
                     /locus_tag="OQQ48_000718"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCS1951581.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLKRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEIRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            22587..23873
                     /gene="damX"
                     /locus_tag="OQQ48_000719"
     CDS             22587..23873
                     /gene="damX"
                     /locus_tag="OQQ48_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HCS1951582.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGDKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            23980..24816
                     /gene="dam"
                     /locus_tag="OQQ48_000720"
     CDS             23980..24816
                     /gene="dam"
                     /locus_tag="OQQ48_000720"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCS1951583.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            24834..25511
                     /gene="rpe"
                     /locus_tag="OQQ48_000721"
     CDS             24834..25511
                     /gene="rpe"
                     /locus_tag="OQQ48_000721"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCS1951584.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            25504..26262
                     /gene="gph"
                     /locus_tag="OQQ48_000722"
     CDS             25504..26262
                     /gene="gph"
                     /locus_tag="OQQ48_000722"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HCS1951585.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALAWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     SEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            26255..27259
                     /gene="trpS"
                     /locus_tag="OQQ48_000723"
     CDS             26255..27259
                     /gene="trpS"
                     /locus_tag="OQQ48_000723"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCS1951586.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            27528..28433
                     /locus_tag="OQQ48_000724"
     CDS             27528..28433
                     /locus_tag="OQQ48_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1951587.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALE
                     LGPHTYVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            28450..28812
                     /locus_tag="OQQ48_000725"
     CDS             28450..28812
                     /locus_tag="OQQ48_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCS1951588.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            28896..30059
                     /locus_tag="OQQ48_000726"
     CDS             28896..30059
                     /locus_tag="OQQ48_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HCS1951589.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQVLENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLSVAHQ
                     GHLVQIPCHQVADAVEQGTDVITVFTLDKAREVSAAAVKAGRVQFVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVVAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLIQAR
                     DQLAKSGIALEQLNAPSATSCTSLPLLAQYGVTHAEPGHALTGTIPANQQGDQSERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     SLAGEFPVSSAVVLCFRTQIFVTRSDVVLVSGIHRGEPKIVGRYDSLGNSLGA"
     gene            30059..31285
                     /locus_tag="OQQ48_000727"
     CDS             30059..31285
                     /locus_tag="OQQ48_000727"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCS1951590.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINALGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFCDVIDRVEQALVSAGWQVERRGDELQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVREQVQVGRVITFGGLLTDSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDKKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICTNIQETDLAGHAEDVARYAERLQVVDRNLARLVDAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPLLVYQQGLAATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            31282..32160
                     /locus_tag="OQQ48_000728"
     CDS             31282..32160
                     /locus_tag="OQQ48_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HCS1951591.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLGVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGLIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRETPSQFFQ"
     gene            32171..32524
                     /locus_tag="OQQ48_000729"
     CDS             32171..32524
                     /locus_tag="OQQ48_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HCS1951592.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGSFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            32536..33840
                     /locus_tag="OQQ48_000730"
     CDS             32536..33840
                     /locus_tag="OQQ48_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HCS1951593.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            33852..34937
                     /locus_tag="OQQ48_000731"
     CDS             33852..34937
                     /locus_tag="OQQ48_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCS1951594.1"
                     /translation="MKTFPLQSLTLIEAQQKQFALVDTICRHFPGAEFLTSGDLGLTP
                     GLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITVDFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVINRIRA
                     TLYSGGSQIQGAQALEVLRCLVFAPVMHAVQAGVSERLLALLNGGEVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQSE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(35089..35820)
                     /gene="phoB"
                     /locus_tag="OQQ48_000732"
     CDS             complement(35089..35820)
                     /gene="phoB"
                     /locus_tag="OQQ48_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HCS1951595.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCKKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(35920..36705)
                     /gene="frlD"
                     /locus_tag="OQQ48_000733"
     CDS             complement(35920..36705)
                     /gene="frlD"
                     /locus_tag="OQQ48_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HCS1951596.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDETLRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            complement(36702..37532)
                     /gene="frlC"
                     /locus_tag="OQQ48_000734"
     CDS             complement(36702..37532)
                     /gene="frlC"
                     /locus_tag="OQQ48_000734"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HCS1951597.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAMDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELYEYAENIGMDLILEPLTPYESNIVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIERGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            complement(37582..38604)
                     /gene="frlB"
                     /locus_tag="OQQ48_000735"
     CDS             complement(37582..38604)
                     /gene="frlB"
                     /locus_tag="OQQ48_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HCS1951598.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQAAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            complement(38625..40013)
                     /gene="frlA"
                     /locus_tag="OQQ48_000736"
     CDS             complement(38625..40013)
                     /gene="frlA"
                     /locus_tag="OQQ48_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HCS1951599.1"
                     /translation="MTANSPLQRVGQEKGIAMGSQELQRKLGFWAVLAIAVGTTVGSG
                     IFVSVGEVAKAAGTPWLTVLTFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGS
                     RPLAFLSGWASFWANDAPSLSIMALAIVSNLGFLTPIDPLPGKFIAAGLIIAFMLLHL
                     RSVEGGAAFQTLITIAKIIPFTIVIGLGIFWFKAENFAAPTTTAIGATGSFMALLAGI
                     SATSWSYTGMASICYMTGEIKNPGKTMPRALIGSCLLVLVLYTLLALVISDLMPFDKL
                     ANSETPISDALTWIPALGSTAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFF
                     KCFGHVHPKYNTPDVSIILQGALGLFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWC
                     RKRDDYKPLWRTPAFGLMTTLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAF
                     WAKRSRQLNALS"
     gene            complement(40258..40425)
                     /locus_tag="OQQ48_000737"
     CDS             complement(40258..40425)
                     /locus_tag="OQQ48_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HCS1951600.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
BASE COUNT         9346 a        10255 c        11119 g         9706 t
ORIGIN      
        1 ttgtggaata aaaaggccgg agatattctc cggcactttt atgtcaggca aggcgttcgt
       61 tgccagatgc ggcgtgaacg ccttatcgcg caggtttagg ggccagtccg ggcgtttaac
      121 gttttttgcc cattgccgcc gccagcgcat ccatcatcgc gctattaccg gcaggctgcg
      181 cttcacgacc gcgtggtttg gctgccgggc ggttgttttg cgggcgttca ttaccgccgc
      241 cgcgacgggc gttggtttcg ccaggctgct cgtccagacg catagtcagg gcgatacgtt
      301 tacgctgaag atccacttcc agcactttca ccttcacaat gtcgcccgct ttcaccacgg
      361 tatgcggatc ttccacaaac ttgttcgaca atgaagagat gtgaaccagg ccgtcctgat
      421 gcacgccgat atcgacaaac gcgccaaagt tggtgacgtt ggtcaccgca ccttcgagga
      481 tcatacccgg ttgcaggtcg ttcattgtct cgacgccatc ggcaaactga gcggttttaa
      541 attccggacg cggatcgcga cccggttttt ccagctcttt gatgatgtca gttactgtcg
      601 gcacaccgaa tttttcatca gtaaagtcag acgctttcag gttacgcagt tcgctgctgt
      661 tacccatcag atctttcagt gcctgctgtg ttgctgccag aatgcgttcc accaccggat
      721 aggcttccgg gtggacggta gaagcatcca gcgggttatc accgtggtta atgcgcaaga
      781 agcccgcgca ctgctcgaag gctttcggcc ccagacggct gactttcagc agttgctgac
      841 ggttctggaa ctggccgttc tcatcgcgcc aggccacgat gttttgcgcc atcatgcgcg
      901 tcaggcccgc cacgcgggtt aacagcggaa cagaagcagt gttgagatcg acgccaacgg
      961 cgtttacgca gtcttctact actgcgtcca gtttgcgggc cagttgcgtc tggctgacgt
     1021 catgctgata ctgacctacg ccgatagatt tcggatcgat tttcaccagc tccgccagcg
     1081 gatcctgcaa acggcgggcg atagacaccg cgccacgcag cgaaacgtcg agatccggga
     1141 actcctgcgc tgccagctcg gaagctgagt aaaccgacgc gccagcttcg ctgacgatca
     1201 ctttctgtgc ggtcactttc gggaactgct tctgcacgtc gagatagaaa cgttcagttt
     1261 cgcgggaagc tgtaccgttg ccgatcgcca ccagttcaac gttatgtttt tcacacaagg
     1321 cagcaacggt catcgctgct tttgcggctt gtccggtgtg cgggtaaatg gtatcggtcg
     1381 ctaccagttt gccagtggca tcgaccaccg ccacttttac cccagtacgc agacccggat
     1441 cgaggcccat cgttgcacgc agtccggcag gggccgccat cagcagatcg tgcaggttac
     1501 gggcaaagac gttgattgct tcatcttccg cgcgttcgcg cacggtaccc atcagttcgg
     1561 tttccagatg catcagcacc ttgatgcgcc aggtccagct cactacgcct ttgcgccagc
     1621 tatccgccgg ggcattgttc aggcgcaggc caaggtgatc catgatgatt tgctcgcaat
     1681 agctctcttt gggcggctca tcgaactgcg gatcggcatt cagcgaaagc tggagtacgc
     1741 cttcgttacg cccacggaac atcgccagag cgcggtgaga aggcaccgtg gacaacggtt
     1801 cgtgatgatc gaaatagtcg cggaatttcg ccccttcctc ttctttaccg ctcaccaccg
     1861 tagaaaccaa atgcgcgttc ttccacagat aatcacgcac tttcgccagc agcgcggcat
     1921 cttcggcaaa ccgttccatc aggatatagc gcgcgccatc cagcgcggct ttggtatctg
     1981 ccacgccttt atcggcatca acatattgtg cagcggcgac ttctggcgtg tgtgacggat
     2041 cgctccacag caggtcagcc aacggctcaa gccctgcttc aatggcgatt tgcccgcggg
     2101 tgcggcgttt aggtttgtag ggcaggtaga ggtcttcgag ttcggttttg cttagggtgg
     2161 cgttgatggc cttcgccaga tcatcggtga gtttgccttg ctcggaaatg gacttgagga
     2221 tcgcctgacg tctctcttcc agctcgcgca gatagctcag acgcgtttcc agattacgca
     2281 gctgcgtgtc atccagaccg ccggtgattt ccttacgata acgtgtgata aacggcacgg
     2341 tattcccttc gtcaagcagg cgaacggcag cgtcaacctg ttccgggcgc gcctgaattt
     2401 cacccgcaat aatgcggcag aacgaatcat tcatcatggt ttgagttcat cttttcggat
     2461 caaaaatcag gggatagtta tacggactgg ctggcaaaaa tgccagccat cggcaggagg
     2521 ttaagactct tccttacggt ttcacgtact cgatagcatt aacataccag ctcgcttccc
     2581 cggcaggggt attcaccacc gccagatcgc cgacttcttt tttcagcaat gcgcgggcca
     2641 tcggggaatc gatagagatg taatctttac ggccaaaaat ttcatcgtag ccgacaatac
     2701 ggaaacggtg agtcacgcca tcgtcgtttt caatctccac ccacgcgcca aaaaagactt
     2761 tgccttcctg ctgaggggag taatcgacga ttttgagatt ttccatgcat ttagtgagat
     2821 agcgcacgcg acggtcgatt tcacgcagac gctttttatt atactgatag tcagcatttt
     2881 cgctgcggtc gcccagactt gcggcccagg tcaccttttt tgtgacctcc gggcgttctt
     2941 cacgccagag ataattaagc tcttgtttga gtttttcata cccttcccgg gtaaccaggg
     3001 gcgttttcat ctcgttgatt ccctttgtct gtttgataat gcgcacattg ggtataacgt
     3061 gatcatatca acagaatcaa taatgtttcg ccgaataaat tgtatactta agctgctgtt
     3121 taatatgctt tgtaacaatt taggctgaaa ttcataccag atttagctgg tgacgaacgt
     3181 gagctttttt aagaatacac gcttacaaat tgttgcgaac ctttgggagt acaaacaatg
     3241 caagagaact acaagattct ggtggtcgat gacgacatgc gcctgcgtgc gctgctggaa
     3301 cgttatctca ccgaacaagg cttccaggtt cgaagcgtcg ctaatgcaga acagatggat
     3361 cgcctgctga ctcgtgaatc tttccatctt atggtactgg atttaatgtt acctggtgaa
     3421 gatggcttgt cgatttgccg acgtcttcgt agtcagagca acccgatgcc gatcattatg
     3481 gtgacggcga aaggggaaga agtggaccgt atcgtaggcc tggagattgg cgctgacgac
     3541 tacattccaa aaccgtttaa cccgcgtgaa ctgctggccc gtattcgtgc ggtgctgcgt
     3601 cgtcaggcga acgaactgcc aggcgcaccg tcacaggaag aggcggtaat tgctttcggt
     3661 aagttcaaac ttaacctcgg tacgcgcgaa atgttccgcg aagatgagcc gatgccgctc
     3721 accagcggtg agtttgcggt actgaaggca ctggtcagcc atccgcgtga gccgctctcc
     3781 cgcgataagc tgatgaacct tgcccgtggt cgtgaatatt ccgcaatgga acgctccatc
     3841 gacgtgcaga tttcgcgtct gcgccgcatg gtagaagaag atccagcgca tccgcgttac
     3901 attcagaccg tttggggcct gggctacgtc tttgtaccgg acggctctaa agcatgaggc
     3961 gattgcgctt ctcgccacga agttcatttg cccgtacgtt attgctcatc gtcaccttgc
     4021 tgttcgccag cctggtgacg acttatctgg tggtgctgaa cttcgcgatt ttgccgagcc
     4081 tccagcagtt taataaagtc ctcgcgtacg aagtgcgtat gttgatgacc gacaaactgc
     4141 aactggagga cggcacgcag ttggttgtgc ctcccgcttt ccgtcgggag atctaccgtg
     4201 agctggggat ctctctctac tccaacgagg ctgccgaaga ggcaggtctg cgttgggcgc
     4261 aacactatga attcttaagc catcagatgg cacagcaact gggcggcccg acggaagtgc
     4321 gcgttgaggt caacaaaagt tcgcctgtcg tctggctgaa aacctggctg tcgcccaata
     4381 tctgggtacg cgtgccgctg accgaaattc atcagggcga tttctctccg ctgttccgct
     4441 atacgctggc gattatgcta ttggcgatag gcggggcgtg gctgtttatt cgtatccaga
     4501 accgaccgtt ggtcgatctc gaacatgctg ctttgcaggt cggtaaaggg attattccac
     4561 cgccgctgcg tgaatatggc gcgtccgagg tacgttcggt tacccgcgcc tttaaccata
     4621 tggcggctgg tgttaagcaa ctggcggatg accgcacgct gctgatggcg ggggtaagtc
     4681 atgacttacg cacgccgctg acgcgtattc gcctggcgac cgagatgatg agcgaacagg
     4741 atggctacct ggccgaatcg atcaataaag atatcgaaga gtgcaacgcc atcattgagc
     4801 agtttatcga ctacctgcgc accgggcagg agatgccgat ggaaatggcg gatctcaacg
     4861 cagtactcgg tgaggttatt gctgccgaaa gtggctatga gcgggaaatt gaaaccgcgc
     4921 tttaccccgg cagcattgaa gtgaaaatgc acccgctgtc gatcaaacgc gcggtggcga
     4981 atatggtggt caacgccgcc cgttacggca atggctggat caaagtcagc agcgggacgg
     5041 agccgaatcg cgcctggttc caggtggaag atgacggtcc gggaattgcg ccggaacaac
     5101 gtaagcacct gttccagccg tttgtccgcg gcgacagtgc acgcaccatt agcggcacgg
     5161 gattagggct ggcaattgtg cagcgtatcg tggataacca taacgggatg ctggagcttg
     5221 gcaccagcga gcggggcggg ctttccattc gcgcctggct gccagtgccg gtaacgcggg
     5281 cgcagggcac gacaaaagaa gggtaaataa acgggaggcg aaggtgcctc ccgttttgct
     5341 ttctataaga tactggatag atattctcca gcttcaaatc attacagttt cggaccagcc
     5401 gctaccagcg cggcacccgc aggggtgtcg gtgtatttat cgaagttgtc gataaacagt
     5461 ttcgccaggg tttcggcttt ttcctgccac tgttccggag aagcgtaggt gttacgcgga
     5521 tcgagaatct tcgtgtctac gcccggcagt tcggttggga tcgccaggtt aaacatcggc
     5581 agagtgaagg tttctgcatt atccagcgaa ccgttgagga tggcgtcgat aatggcgcgg
     5641 gtatctttaa tcgagatacg tttgcctgtg ccgttccagc cagtgttaac cagataagcc
     5701 tgcgcgcccg ccgcctgcat acgtttcacc agcacttctg cgtactgagt cgggtgcagc
     5761 gacaggaatg ccgcgccgaa gcaagcggag aaggttggcg tcggttcggt gatgccacgc
     5821 tcagtaccgg ccagtttggc ggtgaagcca gagaggaagt gatactgggt ttgatcggca
     5881 gtcaggcgag aaaccggcgg caacacgccg aaagcatcag cagtcaggaa gataacctta
     5941 gtcgcgtggc ccgctttgga aaccggctta acgatgttat cgatgtgata gattggataa
     6001 gaaacgcggg tgttctcggt ttttgaacca tcatcaaagt cgatagtgcc atcttcacgc
     6061 acggtgacgt tttccagcag cgcatcacga cggatagcgt tgtagatttc aggttccgct
     6121 tctttcgaca gcttgatagt ttttgcgtag cagccgcctt cgaaattaaa cacgccgtca
     6181 tcgtcccagc cgtgttcgtc atcgccaatc aggcggcgtt tcgggtcggt ggaaagggtg
     6241 gttttaccgg tgccggaaag gccgaagaac accgcaacat cgcctttctc gccaacgttg
     6301 gcggagcagt gcatagaagc gatacctttc agcggcagca ggtagttcat catcgagaac
     6361 atccctttct tcatttcgcc gccgtaccag gtgccgccaa tcagctgcat gcgctcggtc
     6421 aggttaaacg ccacgaagtt ttcggagttc agaccctgtt ctttccactg cgggttagtg
     6481 cacttcgcgc cgttcataac gataaagtct ggtttgaaac ctgccagctc ttcatcgctc
     6541 gggcgaataa acatgttttt gacaaaatgc gcctgccagg ccacttcggt gatgaaacgg
     6601 acggaaagac gagtatccgg gttcgcacca cagaaagcgt cgacaacgaa cagacgtttg
     6661 ccggaaagct gcctggtcac caggcctttc agatgctgcc aggtttccgg agagagaggt
     6721 ttgttgtcgt tcttaccttt gcctttgtct gcccaccaga aagtatcgcg agtggtatcg
     6781 tcacggacga tatacttatc ttttggtgaa cgaccggtga agatcccggt atcgacggca
     6841 acggcaccca gattagttaa caccccgcgc tcataacctg tcaggctcgg atcgagctct
     6901 tcctgataca gcaggtcgta gcttgggttg taaacgatat catgtacgtc actgatacca
     6961 taagcctcga gttcttgcgg ggtcaaacca ttgttaacgc gcatttcact gctccttagc
     7021 caatatgtat tgcctgaata gtaaagtctt tttgggggtg ttaaccgcga caaggctcat
     7081 agatttacgt atctggagaa attcatattt gacaaaaaac cccgtgattc ctgttacgaa
     7141 acggttgcta ttatcgcaga aatagcattt taggtgtgga aaatgttctc aaaaggttaa
     7201 atcttggttt atatggcggg aatattgtga gtggaatcgc attccttaat gaaattgatg
     7261 caaattcaat cggtaaagta tcgattcacc acgctaactt tttttgatta ttttctgaaa
     7321 aaagcaaaat actactgccc ggatgcgata attaacccac cttttgattc tggataacca
     7381 acatggataa cgttgaactt tcaccggcaa cacgctgggg gatgattgct accggattac
     7441 ttcaggggct ggtttgctac ctgttgatcg cctggcttgc cggaaaaaat cacagttgga
     7501 ttgtttatgg cgtgcctgcg accgttgcgt tttcatccgt tttactcttc tccgtgatct
     7561 cttttaaaca gaagcgcctt tggggatggc tggcgctggt gtttattgcc acgacaggga
     7621 tgagcggttg gctgaagtgg caaactgatg gcatgaaccc ctggagagct gaaaaggcga
     7681 tttgggattt tggctgctat ctgctgttga tggcaatgtt gttgctaccg tggatacaac
     7741 aaagccttcg tatccgcaat tgcagtagcc gctatagcta cttttaccag tcagtatggc
     7801 ataacgtact tatattactg gtgatttttc tcgccaatgg cctgacgtgg ctggtgcttt
     7861 tactgtgggg tgagttgttt aaacttgttg gcatcaagtt tttcaatacg ctcttttttg
     7921 caaccgactg gtttatgtat cttacattag gtctggttac cgcactggcg gtgattctcg
     7981 cgcgcacaca gtcacgttta atcgactcta ttcaaaagtt gttcacgtta atcgccacgg
     8041 ggttgctgcc gttagtatca ttgctaaccc tgatgtttat catcactctg ccgtttacgg
     8101 gcctgagcgc gatttctcgc cacatctccg ccgccgggtt gctgttaacg ctggcctttt
     8161 tgcaattgat cttaatggct attgtccgcg atccgcaaaa agcgtcactt ccctggacgg
     8221 ggccgttgcg ttgcctgatt aaaaccgctt tgctggttgc tccgctgtat gtgttcgtcg
     8281 ccgcctgggc gttatggctg cgggtcgctc agtacggctg gactgtcgac cgcttgcagg
     8341 gcgcgctggc ggtgctggtg ttactggtgt ggtcgctggg gtattttgtc agtatcgtct
     8401 ggcgtaatgg gcaaaatccc cttgttcttc agggcaaagt gaaccttgcg gtttcgttat
     8461 tggtgttggt gatactggtg cttcttaatt cgccggtgct ggacagtatg cgtattagcg
     8521 tgaacagtca tatggcgcgt tatcagagcg gcaaaaacac gccagaccag gtaactatct
     8581 acatgctcga acagagcggt cgctatggac gtgcggcgct tgagtcgctg aaaagcgatg
     8641 ccgggtttat gaaagacccg aaacgcgcgc gggatctgct gatggcgtta gatggagagc
     8701 aacatcttca ggaacaggta tcggaaaaag tattagccga aaatgtgtta attgcccctg
     8761 gttctgttaa accggatgtg acattctggt cggccttaat ccaggatcgc tataacgtga
     8821 tgacctgtat tgaaaaagac gcctgcgtcc tggtcgagca agatctgaat agtgatggtc
     8881 aggcggagcg gatcctgttt gcttttaatg atgacagagt cattgtctat ggctttgact
     8941 cagacagaaa agaatgggac gcgcttgata tgagtttact tccgaacgaa ataacgaaag
     9001 aaaaattact cacagctgcg aaggatggaa aactggggac gaggccgaaa gcgtggcgcg
     9061 atctgacggt agatggtgaa acgctggaaa taaacttaag taagtgacaa cgcttttatc
     9121 ggatacgacg ctgtatctga ccaaaataat ttgcgattta tttcataggc gctggtaagc
     9181 gcagcacatc aggccattaa gttgtaaaat ccggcagagc cctctctgcc ggacatactc
     9241 attaatgaac ttgcggatct gccggagacg cgttgttgcg gatttcagca atatccatcg
     9301 cattgaacag atagtggtta ccgcagtaat cacaatgcat gtcaatttcg ccatcttccg
     9361 ccaggatgct atcaacttct tcatcaggca gcgttttcag cgcatcggcg caatgttcac
     9421 gcgagcaggt gcatttgaac tccacatcct gcggatcgta aaccgtcacc tcttcttcgt
     9481 gatacaaacg ccacaacact tcgtttgccg gtaaggtcag cagttcttcg gttttgatgg
     9541 tttcggttag cgtcgccagg tggtcaaagt cgtcctgctg ggcattttgc gcaggcatta
     9601 cctgcaacaa cataccgcct gcagccggtt tgccgtctac gtcgccggtg cgaataaaca
     9661 ggcgcgtcgg cagctgttca gaacgcataa agtaatcttc caggcaggcc gccagggtat
     9721 caccttccag accaactacg ccctgatagc gttcgccttc gctcggggta atggtgatca
     9781 ccacgtaacc attgccgacc agcgttttca ggtcggcatt ttctggaatt tcgccctgca
     9841 cgcgcgccac accgcgcatt tgctggttat tgttaccgtt aataaccgcc agattcatcg
     9901 gaccgtcgcc ctgcagctgt acggtgatat caccatcaaa cttcagcgta gcggttaaca
     9961 ggctggtcgc aaccagcagt tctgccagca cgtttttaac gggctgcgga taatcgtggt
    10021 tctcaaggat ctgttgcagg gtttccgaaa cggttaccag ttcgccgcga acggcaaaat
    10081 tttcaaacag atagcgatgt aattggtcat gttgcggcat aatcatctct cttgcaggtg
    10141 acagttattc actgtcgccg tgtttaaatc gtaacaggtc gcggcgctct tttttgtccg
    10201 ggcgtcggtc cgggtgcggc atggttaagg cattaagttt acgtgccatc gccatttttt
    10261 cgcgtttctc tacactttcc gcagtctctt catacagcaa ggctgcctcg ctggcggggc
    10321 gacgctgttc agtaatcgcc tttacaatca ccgtgcgttc gtcatttccc tggcgcagag
    10381 tgagcgtggc attcagctcg acgattttgc tcggcttgct gcgctgcccg ttgtaatgca
    10441 ccttaccgcc ttcaatcatt tcacgggcca gcgcgcgggt tttataaaaa cgggcagccc
    10501 atagccattt atccagtcga acctcaacag caggtttctc tttcatggcg tctccttcac
    10561 attagcgagg ggatcaggcg gcggtagtca ttcagtgacg gatggcgttg atactgtttc
    10621 tcggcaatcc cggaatcagg attagtcacg ccgaggcagt aacgaatacc aaattgcgcg
    10681 gcagcatcga gaatcgcttc gctgtcatca ataaacagcg ttctttcagc tttcagaccc
    10741 gtagcttcgg ccaccgcatg ccataaccgc tgatcctctt tcggataacc aaatgtgtgg
    10801 gtggaaagta ataaatcaag gtgtgcgtcc agacccgtat gctcaagttt taccgccagg
    10861 ttgtgcggat gagcattggt gagcaaaatt cgctgcttac cgctggtttt cagtgcctca
    10921 agaaacggaa tggtatcttc acgcagtacg gcgcgcggtc ccatctcggt ggtcattgca
    10981 cagatatcca gacccagttg ctcactccag taatcaagac agtaccagtt tagcgtatgc
    11041 tgtacgtcgt gatattgctg gcgcatatat tccatcgctt cctgtggcgt aaccccgttt
    11101 ttcgcgcccc atgtttcagg caccagcttt tgccagaaat agttatcgaa ggcgaggtcg
    11161 agcaacgtgc cgtccatatc cagcagaacg gtatctacgt cctgccaggc aatgttgata
    11221 tgcatgaggg aaatctccag agtgaagcaa tttgcgcgac agggtagcat aacctgtcgc
    11281 gcaaacgtgt tattcgataa ggctttctga aggggtgatc agttgcgggt tcaggcagct
    11341 ttcataatag gcctgaatct ccatcatgcg agtgcgatga cgctggtaac gacgccaggc
    11401 ctgtacgcca ttgtaaatgg cgctacccag catcgtcagt agcagcaatg tggtgctgag
    11461 atagcgccac aggccggaac gatccgggat cggatgaagg ccaatatgct gcgtaccatt
    11521 ggcgtcagtg aagatttttg tgacgatacc ttcggcgtta aacggcgtat gcatcagcat
    11581 ttgtgccagt ttctggaaag cgttccactg ttcttgcggc gggtagtcgt aaagtgatgc
    11641 cgaaggccag ggctgatcaa caaaatcgct gccttcgtcg ctgacaatca ggaatccgcc
    11701 gggggccgga ctgtttagtg attgtgccgc tcgcgccgtt tcatgcgtga taaacggcgc
    11761 ggtggaggtt gccaccaggt tatccagcga ttccgcactc accgggcgta ataacacatt
    11821 cacgccatcg agcttcccgg cgttggcgcg ttttaccagc gcgtcccagt ctttactgtt
    11881 gccgagattg accagcgcat ttttcagtcg cacacagtca tctttggcag aacataaatc
    11941 cgctgtcttc agtacaatgt cgccaaaatc atcaagcaat accatgccgg atttttgaat
    12001 cgctgaacgt aatgaggcac tgacgcgaga ttcatcttcc ggtttagggt gcagctggcg
    12061 attaactgct tcagtcaatg ccgtcgcttt gttgaccagt tcagattctg gtaatggtaa
    12121 tgagcgggcg tcattccaga tgatctgcga gcagtcaaac ggtaaaaaag gtgaattggt
    12181 tttcgcgctc caggttccgg aagtgcgaat attacacatt cccgtaccgc taatacgcaa
    12241 tgtatcgcct acccgcacgc cagcgtcagc cagttgtttt acgctggtgg cttcaatggt
    12301 ctgcgcgcct ttcatccatg agagagtgaa tttcagcggc atatccagcg ggatccagaa
    12361 taacagcata aacagcacca gcagcgagcc cgcagcgata atcgtactgc gcagccagtg
    12421 ctgtaacgga aagtttttta cttcatcatg cagcgaaaga tatcgcccct gacgcactac
    12481 gtggcagtcg agatagatat caatatcggt ttgttgatcg agatcctgag caatgtatgg
    12541 ctgccagtgt gcgggataga ccaggtcgat aataccgagt gaaatattgt tgatctgttc
    12601 ctgatcgttt tcgccaaaca atccccaacg acggggtgta ccgcgcagac aatgaatttc
    12661 ccgcagggag gattttgccg ggggcgcgaa tagcccccac agacctgcgc ccagcagcag
    12721 taatgcgccg cctgccagcc acggaacaaa tacatccggg gtaatcaggc agaaaaagaa
    12781 catcaggaag gaggcgacga tcagcaacgc ttcacgcagc ccgcgcggac gactcagcgc
    12841 atattcttca tgcgtttctt tgcgaatatt gagtagctcg atttgctcac tctcttcccc
    12901 gcgaatcgac gcctgcgtgg acggaacagg ttgcaaggca tagccgcgcg tttcctgcat
    12961 gtactcctgt agcgtatgac cgttgagtga aataaccaac ggcagcgaat cggtatgaat
    13021 cagttcaacg gtattctcat cgttgatgta ctgttcccag aaggggggca ggtggacttc
    13081 taccgaatcg aggtagtaac gccatttatt cggatcgtcg gtagagatgc cgtaacgtgt
    13141 gatagcgtgt gtcagcatca tgacgttgtt gctttcggca ttcagcgcca gagagatcgg
    13201 tgccgcgcta gctcccgttg ggccaggcac ctgtaatacc tgcgtcaggc tctcaagata
    13261 attttcaacg gcgctacgtt cttcaggtgt gagtttacgc gtttgcgcat ccgcgaaggc
    13321 gttggtccag ggcagctgac tccgtctgga tcgcactttt atcagccatc ccgcaagtag
    13381 tgagcaggcc agcaaagcag ctaaaaaaat cacaatggtg ctcatgcttt ccccatctta
    13441 cttagtctgt caggcgtggt cagtcgcacc agacacaata catgattctg cggttggcta
    13501 tcggcaagcg tggagtttaa cttgagcatc attaagcaca tcacagtaac cgctgatcat
    13561 agcaaaccct ctgaaggcgg aatatcagca aattcctaga gttatttcaa cctattgact
    13621 tttaatttga tttgaggacg attatttatt aagttacata aatgtaaata acatgcaatt
    13681 cacattgccg aaagcgtgtc cgatatcgca caataaccgc tgatttcaca gcatagatcc
    13741 cacgatgagc aaatcattac aaaaacccac cattctgaat gttgaaactg tagcccgttc
    13801 ccgactgttt accgtcgaga gcgtggatct ggagttcagc aatggcgtgc ggcgtgttta
    13861 tgaacgaatg cgtccaacca accgggaagc agtgatgatt gtgccgattg tggacgatca
    13921 cctgatcctg atccgcgaat acgcagtggg aactgaatcc tacgaattag gtttttcgaa
    13981 aggattaatt gatccgggtg aaagcgtcta cgaagccgct aaccgcgagc taaaagaaga
    14041 ggttggattt ggtgcgaacg atctgacttt tttgaagaag ctcagcatgg caccgtctta
    14101 cttttccagc aaaatgaata tcgtggtcgc gcaagatctc tacccggaat cgctggaagg
    14161 cgatgagcca gagccgctac cacaggtgcg ctggccgctg gcgcatatga tggatttgct
    14221 ggaagaccct gacttcaacg aagcgcgtaa tgtcagcgcg ctgttcctcg tgcgcgaatg
    14281 gttgaaaggg caggggcgag tgtaaatgcg tgaagtttgc ctgatgcaca acgcttatca
    14341 ggcttatggg gttcctgcaa tatattgatt tttcacgatt ttgttagccg ggtaacgcgg
    14401 tcacccgcat ccagcataaa caaatgcact ttgtcagcca actgaaaagg cgccgaagcg
    14461 cctttttaat cagaacaatt cctgtgcctc accattatcg ataatggtcg ttcccacctc
    14521 gtgcaccgcc tgttgtgtcg gctgcgtacc ttcgatgaaa tactcttcgc ggctgttgcc
    14581 accattagct aactgcccgg tgctgcgatc gatattcacc gtcacaatac ccggtggcgg
    14641 cgtcagcggc tgctccggca caccttcaag aacggctttc atataagcgt cccatgcagg
    14701 ctgggcactc ttggctccgc cttcgtaacc tgagatctga tctttaatcg ctccggaagc
    14761 cgttgtatga ccgagattac gacggtgatc atcaaagcca atccagaccg aggtcacaac
    14821 gcccggaccg taacccgaga accacgcatc tttcgaactg ttagtggtac cggttttccc
    14881 gccgatatcg cgacgctgca aatcacgacc tgcacgccag ccagtaccct gccagcctgg
    14941 ctcaccaaag atattggtgt tcaaagcact cttaatcagg aatgccagcg gagtgttgat
    15001 gacgtgcggt gcgtactcct gcgcgccagt cttcgccact aacgcctgat ttgcctgctc
    15061 cagctgcggc attggtacag aaacattctg ctgctcgcgg gagatagcga catcttcaac
    15121 atcgttattt tccagcacgt tcgatttctg cgtatcaccg taaatcaccg gaatatcgca
    15181 ttccgggcag gctactttcg gtttcgcttc gaaaatcacg ccgccctgat cgttttcaat
    15241 tttgctgata aaccacgggt ccaccaggaa gccgccgttc gccatgaccg cgtagccgcg
    15301 cgccacctgc attggggtga aggacgctga acccagcgcc agcgattcgg tgtggacaat
    15361 gttttgcgcc gggaagccga agcgttgcag atattctgca gcgtagtcga cgcccatagc
    15421 ccgcattgca cgtaccatca ccacgttttt cgactgaccc agcccctgac gtaagcgaat
    15481 tggaccagca tattgtggcg gcgagttttt cggctgccag tcagaaccgg cacctgcgtc
    15541 ccagcgagaa attggcacat cgttcaacat acttgccagc gtcagacctt tatccatcgc
    15601 cgcggtgtag aggaacggtt tgatgttgga acccacctga cgcagtgcct gggtggcgcg
    15661 gttaaacttg ctctgattga aatcaaagcc accgaccagc gccataacgg caccgttttg
    15721 cggattgatc gacaccagcg ccgagttcac ttccggcact tgtgccagcc accatgcatc
    15781 gccaacctga cgaacccaga tttgctgacc cgtttgcaga acatcggtca ctttacgcgg
    15841 cgtcggtccc tgctgagtat ccgaacggta aggacgcgcc cagcgaacgc cttccatact
    15901 caatgcgacg gtcgacccgt ccgccagcat cgccgtcgct ccctgaggat tggcgctggt
    15961 gactgcggca ggcagcagcg gaccataggt cggcagcgcc ttcagcgtat cggtaatctt
    16021 gttgttatcc cacgccgact cgcccacttt ccacagcaca tttgccgggc cgcgatagcc
    16081 gtggcgcatg tcgtagtcca gcacgttatt acgtaccgcc tgctgtgcgg cctgctgcac
    16141 tttgcgggtg atggtggtgt aaatgcgata accgtcttca taggcacttt cgccataacg
    16201 gttatacatc tcctggcgca ccatttcgct caggtacggc gcagagaaag caatctccgg
    16261 cgcgtgatag ttagcgttaa tcgcctcagt gcgtgtctga tcgaactgtt gttgggtgat
    16321 atacccttca tccagcatcc gcgacagtac gacgttacgc cgcgcgacgg cacgatccat
    16381 tgagtagagc ggattaaagg tggaaggcgc tttcggcagc ccggctatca cagccatttc
    16441 gttcagcgtc agttggtcga ccgttttacc gaaatagact tgtgccgcag caccgacacc
    16501 ataggcgcgg taaccgaggt aaatcttatt cagataaagc tcgaggatct cgtctttcgt
    16561 cagcaactgt tcaatgcgaa tcgcgaggaa gacttcctta atcttacgca tcagcgtgcg
    16621 ttctggactg aggaagaagt ttctcgccag ctgctgggta atggtactcg ccccttgtga
    16681 cgcgtggccg gagaacagcg ccacacttgc cgcacggaag atccccaccg ggtcaacgcc
    16741 gtgatgctcg tagaagcggc tgtcttccgt cgcgataaag gctttcacca tctccggcgg
    16801 gatttgatcc aacgtaaccg gaatacgacg tttctcaccg tattgagcaa tcagctcgcc
    16861 atcggcgctg taaatctgca tcggaatttg caggcgaaca tcttttaatg tcgccacatc
    16921 cggcagttgt ggctcgatgt agcggtatag gccataaatc gagcctgctc ccagcagaat
    16981 gcaacagact gcaaggatca aaaaatactt tacgaacttc actggaaatt tcccatttag
    17041 tttcatttgg gcagtttata aacaaacgcg cggtagtata aaggcaagcc agacgcattg
    17101 atatacccgt cagagtgacg ggtgataagg agatcatcac aatggcattt aagatctggc
    17161 aaattggttt gcatttacaa cagcaagaag cggtagcggt tgcgatcgtg cggggcgcaa
    17221 aagaatgctt tttgcaacgc tggtggcggt tgccgctgga gaacgacatt atcaaagatg
    17281 ggcggattgt tgatgcgcag cagctggcta aaacgttgtt accttggagt cgcgaactgc
    17341 cgcagcgtca tcacattatg ttggcgtttc ccgccagtcg cacattacag cggtcatttc
    17401 cgcgcccgtc gatgtccctt ggtgagcggg agcaaacggc ctggctgtca gggacgatgg
    17461 cccgcgagct ggatatggat ccggactccc tgcgcttcga ttacagcgaa gactcactca
    17521 gtcccgccta taacgtgact gccgcgcaaa gcaaagagct ggcaacgctg cttactctgg
    17581 cagaaaggtt gcgtgttcat gttagtgcga tcaccccgga tgccagtgca ttacagcgat
    17641 tcctgccttt tttaccttct catcagcaat gtctggcctg gcgtgataac gaacagtggc
    17701 tgtgggcgac gcgctatagc tgggggcgca aactggcggt ggggatgact agcgcgaagg
    17761 agctggcggc agcgttattc gttgatcccg acagcgtcgc gatatgtggc gaaggcggat
    17821 ttgacccctg ggaggccgtt tctgttcgtc agccgctgct accgccgcct ggtggagact
    17881 ttgccatcgc gctggggctg gcgcttggga aggcgtactg atgaacccgc caattaattt
    17941 tttgccctgg cgacagcaac gccggaccgc ttttctgcgt ttctggttgc tgatgttcgt
    18001 tgcgcctctg ctgctggccg tcgggataac gctaatactg cgtctgacag gcagcgccga
    18061 agcgcgcata gacgcagttt tgcttcaggc ggaacaacaa ctcgcccgca gcttacagat
    18121 aacgaagcca cgtttgctgg agcaacagca gttacgcaaa cagcgttcgc aacggcagcg
    18181 ccagcgacaa tttacccgcg actggcaatc tgcgctggaa gcactggcgg cgcttttacc
    18241 cgagcacgcc tggctgacaa cgataagctg gcagcaggga acgctggaga tcaaggggct
    18301 gacaacaagc attaccgcgt taaacgcact agaaacgtca ctccgccagg atgcgtcttt
    18361 tcatctcaat cagcggggag ccacgcagca ggatgcgcag ggacgctggc aatttgagta
    18421 tcagttaaca aggaaggtta gcgatgaaca tgttctttga ctggtggttc gccacatcac
    18481 cccgcctccg ccagttttgc tgggcattct ggttgctgat gttagttacg ctcatttttc
    18541 tgtcatcgac acaccatgaa gagcgcgacg cattaattcg attacgggca agtcatcacc
    18601 agcagtgggc cacactgtat cgcctggttg acaccactcc cttcagcgaa gaaaaaacgc
    18661 tgcccttttc gccactggat tttcagttgc ccggcgcgca actggtttcc tggcatccat
    18721 ccgcgcaggg aggtgagttg gcgttgaaaa cgctgtggga agcagtgccg tcggcattca
    18781 cacggctggc agagcgcaac gtcagcgtga gccgtttttc gttaagcgtg gaaggtgatg
    18841 atcttttgtt cacgctacaa ctggagacgc cgcatgaggg ttaaacgctg gttgttggca
    18901 ggtattgcat tgtgcctttt aaccggtatg cgtgaccctt ttaaaccgcc ggaagatcta
    18961 tgccggatta gcgaacttag ccagtggcgc tatcagggga tggtagggcg aggcgagcgc
    19021 atcatcggtg taataaaaga cgggcaaaag aaatggcgac gggtgcagca aaacgatgtg
    19081 ctggaaaacg gctggacaat tttacagctg acgccagacg tactaacgct gggtaccggg
    19141 acaaactgcg aaccgccaca atggttgtgg caacggcaag gagatacaaa tgaagcaatg
    19201 gatagccgca ctactgttga tgctgatacc cggcgtacag gcggcaaagc cgcaaaaagt
    19261 gacgctgatg gtggatgacg ttccggtagc tcaggtgttg caggcgctgg ctgaacagga
    19321 gaagttgaac ctggtcgtgt cgccagacgt cagcggtacg gtgtcgttac atctaacaga
    19381 tgttccctgg aagcaggcac tacaaactgt agtgaaaagc gccggactga taacgcggca
    19441 ggaaggcaac attctctcag tgcattccat tgcctggcag aataacaata tcgcccgcca
    19501 ggaggcggag caggcgcggg cgcaggcaaa tctgccgctg gaaaatcgca gtataaccct
    19561 gcaatacgcc gacgcgggag aactggcgaa agcgggggag aagctactga gtgccaaagg
    19621 gagtatgacc gtcgataaac gcaccaatcg ccttttgcta cgagataaca aaacggcgtt
    19681 aagcgcgctt gaacagtggg tagcgcaaat ggatctgccg gtcgggcagg ttgagctgtc
    19741 ggcgcatatt gtcaccatta atgaaaaaag tttgcgtgag ttaggcgtga aatggacgct
    19801 ggccgatgcg caacacgctg gtggcgttgg gcaagtcacc acgcttggta gcgacctctc
    19861 cgtagcgacg gcgacaacgc atgtcggttt taacattggg cgcatcaacg gacgcttgct
    19921 ggatcttgag ctttccgcgc tcgaacaaaa acagcagctg gatattatcg ccagtccgcg
    19981 tctgctggcc tcacatcttc agcctgccag cattaaacag gggagcgaaa ttccatatca
    20041 ggtttccagc ggggaaagtg gcgcgacgtc ggtggaattt aaagaggccg tcctggggat
    20101 agaggtcacg cccacggtgt tacaaaaagg tcgcatccgg ctgaaattac acatcagcca
    20161 gaacgttccg gggcaggtgc tacagcaggc cgatggcgaa gtgctggcga ttgataagca
    20221 ggagatcgaa acgcaggtcg aggtcaaaag cggagaaacg ttggcgctgg gcggcatttt
    20281 tacccgtaaa aataaatcgg gtcaggatag cgtaccgttg cttggcgaca ttccctggtt
    20341 cgggcaatta tttcgtcatg acggaaaaga atatgaacga cgcgagttag tggtgtttat
    20401 cacgccacga ctggtttcca gtgagtaaac agccgtaaaa gcggtaatgt ttttatgctg
    20461 aacgtgtttc atctatttga cgcgcgcagg tatttagcat acaaggagta ccgatttgag
    20521 agttggcgct cttcgctgcc tgcgttccat gatgatgatt tatcattcag gcggcatttt
    20581 gctgtctttt ttacgctaat cttacccggt gatttatcgc cagagcggtg gtagcaaggc
    20641 agcgcgcttg cagcgaccag atatgcagag ggatgggtga tttattcagt tgccaaaccc
    20701 gctggagtat tgagataatt ttcagtctga ctctcgcaat atcttatgag gtttcagttc
    20761 atgtcctgcg gcgctctctg agcgaagcgg gtttatcatt aacgaatagt cttagtagta
    20821 ccgaaaaaat ggcagagaaa cgcaatatct ttctggttgg gcctatgggt gccggaaaaa
    20881 gcactattgg gcgccagtta gctcaacaac tcaatatgga attttacgat tccgatcaag
    20941 agattgagaa acgaaccgga gctgatgtgg gctgggtttt cgatttagaa ggcgaagaag
    21001 gcttccgcga tcgcgaagaa aaggtcatca atgagttgac cgagaaacag ggtattgtgc
    21061 tggctactgg cggcggctct gtgaaatccc gtgaaacgcg taaccgtctt tccgctcgtg
    21121 gcgttgtcgt ttatcttgaa acgaccatcg aaaagcaact tgcacgcacg cagcgtgata
    21181 aaaaacgccc gttgctgcac gttgaaacac cgccgcgtga agttctggaa gcgttggcca
    21241 atgaacgcaa tccgctgtat gaagagattg ccgacgtgac cattcgtact gatgatcaaa
    21301 gcgctaaagt ggttgcaaac cagattattc acatgctgga aagcaactaa ttctggcttt
    21361 atatacactc gtctgcgggt acagtaatta aggtggatgt cgcgttatgg agaggattgt
    21421 cgttactctc ggggaacgta gttacccaat taccatcgca tctggtttgt ttaatgaacc
    21481 agcttcattc ttaccgctga aatcgggcga gcaggtcatg ttggtcacca acgaaaccct
    21541 ggctcctctg tatctcgata aggtccgcgg cgtacttgaa caggcgggtg ttaacgtcga
    21601 tagcgttatc ctccctgacg gcgagcagta taaaagcctg gctgtactcg ataccgtctt
    21661 tacggcgttg ttacaaaagc cgcatggtcg cgatactacg ctggtggcgc ttggcggcgg
    21721 cgtagtgggc gatctgaccg gcttcgcggc ggcgagttat cagcgcggtg ttcgtttcat
    21781 tcaagtcccg acgacgttac tgtcgcaggt cgattcctcc gttggcggca aaactgcggt
    21841 caaccatccc ctcggtaaaa acatgattgg cgcgttctac cagcctgctt cagtggtggt
    21901 ggatctcgac tgtctgaaaa cgcttccccc gcgtgagtta gcgtcggggc tggcagaagt
    21961 catcaaatac ggcattattc ttgacggtgc gttttttaac tggctggaag agaatctgga
    22021 tgcgttgttg cgtctggacg gtccggcaat ggcgtactgt attcgccgtt gttgtgaact
    22081 gaaggcagaa gttgtcgccg ccgacgagcg cgaaaccggg ttacgtgctt tactgaatct
    22141 gggacacacc tttggtcatg ccattgaagc tgaaatgggg tatggcaatt ggttacatgg
    22201 tgaagcggtc gctgcgggta tggtgatggc ggcgcggacg tcggaacgtc tcgggcagtt
    22261 tagttctgcc gaaacgcagc gtattataac cctgctcaag cgggctgggt taccggtcaa
    22321 tgggccgcgc gaaatgtccg cgcaggcgta tttaccgcat atgctgcgtg acaagaaagt
    22381 ccttgcggga gagatacgct taattcttcc gttggcaatt ggtaagagtg aagttcgcag
    22441 cggcgtttcg cacgagcttg ttcttaacgc cattgccgat tgtcaatcag cgtaacaaca
    22501 agaaaggtca ggccgcttat caagcggtct attagcttca ggttaattgc aacgtggtaa
    22561 gcattaacct tttagtgggg tgttaaatgg atgaattcaa accagaagac gagctgaaac
    22621 ccgatcccag cgatcgtcgt actggtcgtt ctcgtcaatc ttctgaacgt tctgagcgta
    22681 ctgaacgtgg cgaaccgcag atcaattttg atgatattga acttgatgac actgacgatc
    22741 gccgtccgac tcgtgcgcaa aaagagcgca atgaggaacc ggaaatcgaa gaagaaattg
    22801 acgaatccga agatgaaacc gtggatgaag agcgcgtaga gcgtcgtccg cgtaagcgca
    22861 aaaaagcagc cagtaaaccc gcttctcgtc agtatatgat gatgggcgtc ggcattctgg
    22921 ttctactgct gttgatcatc ggtatcggtt ctgcgctaaa agccccctcg acctcttcca
    22981 gcgatcaaac cgcgtctggc gataagagta ttgatcttgc aggcaatgcg accgatcagg
    23041 cgaatggggt gcagccagcg ccgggaacca cgtctgcgga aaatactcag caggatgttt
    23101 ctctgccacc gatctcttct acgccgactc aagggcaaac cccggcggca acggatggtc
    23161 aacaacgtgt tgaagtgcag ggtgacctga acaatgcgct gactcagcca caaaatcagc
    23221 aacagttgaa caatgtggcg gtcaattcca cattgccgac cgaacccgcg actgtcgcgc
    23281 ctgttcgcaa tggcaatgca tcgcgtgaca cggcgaaaac gcaaaccgct gaacgtccgt
    23341 ccactacgcg cccagctcgt cagcaggcgg tgattgaacc gaaaaaaccg caagcaaccg
    23401 tgaaaacgga gccgaagccg gtagcacaga cgccgaagcg tactgaacca gctgcccctg
    23461 tggcgagcac gaaggcaccg gctgcgactt ctacgccagc accaaaagag acggcgacta
    23521 cggctccagt acagacggca tccccggcgc aaaccacggc aacaccagcc gctggaggga
    23581 agaccgcagg taatgttggt tcgttgaaat cggcaccgtc cagccattac actctgcagc
    23641 tgagcagttc ctctaactac gacaacctga acggttgggc gaagaaagag aatctgaaaa
    23701 actacgttgt ctatgaaacg acgcgtaatg gtcagccgtg gtatgtcctg gtttctggcg
    23761 tgtacgcttc gaaagaagag gcgaaaaaag cggtatctac attgccagca gatgttcagg
    23821 ccaaaaaccc gtgggcgaaa ccgctgcgtc aggtacaggc cgatctgaag taatcaaggt
    23881 tatctcccgc aatggtttat cgttgcggga gctgcctgaa gcgctggatg ctgtcggagc
    23941 tttctccaca gccggagaag gtgtaattag ttagtcagca tgaagaaaaa tcgcgctttt
    24001 ttgaagtggg cagggggcaa gtatcccctg cttgatgata ttaaacggca tttgcccaag
    24061 ggcgaatgtc tggttgagcc ttttgtaggt gccgggtcgg tgtttctcaa caccgacttt
    24121 tctcgttata tccttgccga tatcaatagc gacctgatca gtctctataa cattgtgaag
    24181 atgcgtactg atgagtacgt acaggccgca cgcgagctgt ttgttcccga aacaaattgc
    24241 gccgaggttt actatcagtt ccgcgaagag ttcaacaaaa gccaggatcc gttccgtcgg
    24301 gcggtactgt ttttatattt gaaccgctac ggttacaacg gcctgtgtcg ttacaatctg
    24361 cgcggtgagt ttaacgtgcc gtttggccgc tacaaaaaac cctatttccc ggaagcagag
    24421 ttgtatcact tcgctgaaaa agcgcagaat gcctttttct attgtgagtc ttacgccgat
    24481 agcatggcgc gcgcagatga tgcatccgtc gtctattgcg atccgcctta tgcaccgctg
    24541 tctgcgaccg ccaactttac ggcgtatcac acaaacagtt ttacgcttga acaacaagcg
    24601 catctggcgg agatcgccga aggtctggtt gagcgccata ttccagtgct gatctccaat
    24661 cacgatacga tgttaacgcg tgagtggtat cagcgcgcaa aattgcatgt cgtcaaagtt
    24721 cgacgcagta taagcagcaa cggcggcaca cgtaaaaagg tggacgaact gctggctttg
    24781 tacaaaccag gagtcgtttc acccgcgaaa aaataattct caaggagaag cggatgaaac
    24841 agtatttgat tgccccctca attctgtcgg ctgattttgc ccgcctgggt gaagataccg
    24901 caaaagccct ggcagctggc gctgatgtcg tgcattttga cgtcatggat aaccactatg
    24961 ttcccaatct gacgattggg ccaatggtgc tgaaatcctt gcgtaactat ggcattaccg
    25021 cccctatcga tgtacatctg atggtgaaac ccgtcgatcg cattgtgcct gatttcgctg
    25081 ccgctggtgc cagcatcatt acctttcatc cagaagcctc cgagcatgtt gaccgcacgc
    25141 tgcaactgat taaagaaaat ggctgtaaag cgggtctggt atttaacccg gcgacacctc
    25201 tgagctatct ggattacgtg atggataagc tggatgtgat cctgctgatg tctgttaacc
    25261 caggttttgg cggtcagtcg ttcattccac aaacactgga taaactgcgc gaagtacgtc
    25321 gtcgtatcga cgagtctggt tttgacattc gactggaagt ggacggtggc gtgaaggtga
    25381 acaacattgg cgaaatcgct gcggcgggcg cggatatgtt cgtcgccggt tcggcaatct
    25441 tcgaccagcc agactacaaa aaagtcattg atgaaatgcg cagtgaactg gcaaaggtaa
    25501 gtcatgaata agtttgaaga tattcgcggc gtcgcttttg atctcgacgg tacgctggtt
    25561 gacagtgctc ccggtcttgc tgctgcggta gatatggcgc tgtatgcgct ggagttaccc
    25621 gtcgcaggtg aagaacgcgt tattacctgg attggtaacg gcgcagatgt tctgatggag
    25681 cgtgcattgg cctgggcgcg tcaggaacgt gcgactctgc gtaaaacaat gggtaaaccg
    25741 cccgttgatg acgacattcc ggcagaagaa caggtacgta ttctgcgtaa actgttcgat
    25801 cgctactata gcgaggttgc cgaagagggg acgtttttgt tcccgcacgt tgccgatacg
    25861 ttgggcgcgt tgcaggctaa aggcctgccg ctaggcctgg tcaccaacaa accgacgccg
    25921 ttcgtcgcgc cactgctcga agccttagat attgccaaat actttagcgt ggttatcggc
    25981 ggcgatgatg tgcaaaacaa aaaaccgcat ccggacccgc tgttactggt ggctgagcgg
    26041 atgggaattg ccccacaaca gatgctgttt gtcggcgact cacgcaatga tattcaggcg
    26101 gcaaaagcgg caggttgccc atcagttggc ttaacctacg gatataacta cggcgaggct
    26161 atcgatctca gccagcctga tgtaatttat cagtctataa atgaccttct gcccgcatta
    26221 gggcttccgc atagcgaaaa tcaggaatcg aaaaatgact aagcccatcg tttttagtgg
    26281 cgcacagccc tcaggtgaat tgaccattgg taactacatg ggtgcgctgc gtcagtgggt
    26341 aaacatgcag gatgactacc attgcattta ctgtatcgtt gaccaacacg cgatcaccgt
    26401 gcgccaggat gcacagaagc tgcgtaaagc gacgctggat acgctggcct tgtatctggc
    26461 ttgtggtatc gatcctgaga aaagcaccat ttttgttcag tcccacgtac cagaacatgc
    26521 gcagttaggc tgggcactga actgctatac ctacttcggc gaactgagcc gcatgaccca
    26581 gtttaaagat aaatctgcgc gttatgccga gaacatcaac gctggtctgt ttgactatcc
    26641 ggtgctgatg gctgcggaca tcctgctgta tcaaactaat ctggtaccgg tgggtgaaga
    26701 ccagaaacag cacctggaac tgagtcgcga tatcgcccag cgtttcaacg cgctgtatgg
    26761 cgagatcttt aaggtgccgg agccgtttat tccgaaatct ggcgcgcgcg taatgtcgct
    26821 gctggagccg accaagaaga tgtccaagtc tgacgataac cgcaataacg ttatcggcct
    26881 gctggaagat ccgaaatcgg tagtgaagaa aatcaaacgc gcggtcactg actccgacga
    26941 gccgccggta gttcgctacg atgtgcagaa caaagcgggc gtttccaacc tgctggatat
    27001 cctttctgcg gtaacgggcc agagcatccc agaactggaa aaacagttcg aaggcaagat
    27061 gtatggccat ctgaaaggtg aagtggctga tgccgtttcc ggtatgctga ctgaattgca
    27121 ggaacgctat caccgtttcc gcaacgatga agccttcctg caacaggtga tgaaagacgg
    27181 cgcagaaaaa gccagtgtac acgcttcccg tacgctgaaa gcggtgtacg aagcgattgg
    27241 ttttgtggcg aagccgtaat atcaaaacgc tcggacggtc ccctcgcccc tttggggaga
    27301 gggttagggt gaggggagca ggcaagcgcc agtgggaatt accctttgct cgcattgtcg
    27361 aataaatcac ttcgtcatca ctctgaaacc ggggaaaccc ggttttttta tcctcatttg
    27421 caataatccg aaaaaatgtg aagcgcctcg ccgtttccac atctcacagt tgccacttat
    27481 cttcatttta atgaagatat aaacattcat tttattgaaa atatattatg cgtcgaacgt
    27541 ttatcaaaaa agaaggcgtc gtcatcacga cgctggcccg ttatttgttg ggtgaaaaat
    27601 gcggtaatcg actgaaaacc atagatgagc tggcaactga atgccgttca tcggttggcc
    27661 tgacgcaggc cgcgttgaaa acgttggaat caagtggagc gatacggatt gaacgccgtg
    27721 ggcgcaatgg cagttatctg gtcgaaatgg ataacaaagc gctgctgagt catgtggata
    27781 tcaataacgt tgtgtgtgcc atgcctttgc cctatacccg cttgtacgaa ggtttagcaa
    27841 gcgggctgaa agcccagttt gatggcattc ctttttatta tgcgcatatg cgtggcgcgg
    27901 atattcgtgt ggagtgtttg cttaacggtg tctatgacat ggcagtagtt tcccgcctgg
    27961 cggcggaaag ttatctcagc caaaacaatt tgtgcatcgc cctcgagctt ggcccgcata
    28021 cgtatgttgg tgaacatcaa ctgatatgcc gcaaaggtga gtctggaaat gttaagcgag
    28081 taggtctcga tagtcgttcg gcggatcaga aaatcatgac cgatgtcttt tttggcgata
    28141 gtgatgtgga acgtgtcgat ctctcttatc acgagagttt acaacgcatt gttaaaggcg
    28201 atgtcgatgc ggttatctgg aacgtggtgg cggaaaacga actgaccatg ctgggattag
    28261 aggcgacgcc gctcacagac gatccgcgat ttttacaggc caccgaagcc gtggtcctga
    28321 cgcgagtcga tgattaccca atgcaacaac tgctgcgtgc cgttgtagat aaacacgccc
    28381 tgctcgccca tcaacaacgg gtagtgagtg gggaacagga accgagttat taaacgaaag
    28441 gcatctgata tggaaaccag actcaacctg ctttgcgagg caggcgttat tgataaggac
    28501 atctgcaaag gcatgatgca ggtcgtcaac gtactggaaa cagagtgcca tctgccggtg
    28561 cgcagtgagc aaggaacgat ggcgatgaca catatggcga gtgcactgat gcgcagtcgc
    28621 cgtggtgaag aaatagagcc gctggataac gaattgctgg cagagctggc gcaatccagc
    28681 cactggcaag ccgttgtgca attgcatcag gtgttgttga aggaattcgc actggaagtt
    28741 aatccgtgtg aagaaggcta tttgctggcc aacctttatg gattatggat ggctgctaac
    28801 gaagaggttt gaagctcccg acaaatccac gtcgcacaac ctaaatattc aaaaaaatga
    28861 atatttaatt caaggaacag aaacgaggca acaagatgtt tgtagaagca ttgaaacgcc
    28921 agaacccggc gctgatttcc gccgcactaa gtttgtggca gcagggcaag atcgccccgg
    28981 acagctgggt gatcgacgtg gatcaggtac tggaaaacgg taagcgactg attgagacgg
    29041 cgcggcttta cggcattgaa ctgtatctga tgaccaagca gtttggccgt aacccatggc
    29101 tggcggaaaa attgctggca ttaggttaca gcggcattgt ggcggtggat tacaaagagg
    29161 cgcgagtcat gcgccgcgct ggtttgtcgg tggcgcatca ggggcatctg gtacaaatcc
    29221 cttgtcatca ggttgctgac gccgttgaac agggcaccga cgtcatcacc gtgtttactc
    29281 tcgacaaagc gcgggaagtt tctgcggcag cggtgaaggc cggacgtgtg cagtttgtgc
    29341 tgctcaaagt ttatagcgac gatgattttc tttatccggg gcaggagagc ggttttgtac
    29401 agcattcgct gcacgaggtg gttgctgaaa ttaagaaact gccgggtctg catttagccg
    29461 gacttaccca tttcccttgc ctgctttggg atgaggctgc cggaaaagtt ttgccgacac
    29521 cgaatcttca cacgctgata caggcacggg atcaactggc gaaatctggt attgcacttg
    29581 agcaactgaa cgcgccttca gcgaccagct gcacttcgct gccattactg gcgcaatacg
    29641 gtgtgactca tgccgaaccc ggtcatgcgc tgacgggcac tattccggca aaccagcagg
    29701 gcgatcagtc tgaacgtatc gcgatgctct ggttgagtga aatctcccat catttccgtg
    29761 gcgacagcta ctgctacggc ggcggttact accgtcgtgg tcatgcgcaa catgcgttgg
    29821 tgtttacgcc agaaaatcaa aagattactg aaaccaatct caaaactgtg gatgacagca
    29881 gtatcgacta caccctgtcg ctggcaggcg agtttccggt aagcagtgca gtggtgctct
    29941 gtttccgcac gcagattttt gtcacccgta gcgatgtggt gctggtgtcc ggtattcacc
    30001 gtggcgaacc gaaaatcgtt ggtcgttatg acagtcttgg aaactctctg ggggcgtaat
    30061 ggcgcgattt gtggtgttag tgattgatag ctttggcgta ggggcaatga aagatgtcac
    30121 gctggtgcgt ccgcaagatg cgggagcgaa tacatgtggt cacatcctga gccagttgcc
    30181 gcatttgcag ctaccaacgc tggagaagct ggggctaatc aacgcattgg gttatgcgcc
    30241 aggcgatatg cagccgtcag attccgcaac ctggggcgtg gcagagctgc aacatgaagg
    30301 tggcgatacc tttatggggc atcaggaaat tttaggcacg cgcccgttac cgccgctgcg
    30361 gatgcctttt tgcgatgtga ttgaccgtgt tgagcaggca ttagtatccg ccggttggca
    30421 ggtggagcgc cgtggcgatg aactgcaatt tctgtgggtc aatcaggcgg ttgcgattgg
    30481 cgataatctc gaggcggatt taggccaagt ctataacatt accgccaatc tctctgtgat
    30541 ctcttttgac gacgcaatca aaattggtcg tatcgtgcgt gagcaggtac aggtcggtcg
    30601 ggtcattaca tttggtggcc tgttaaccga cagtcaacgc attctcgatg ccgcagaaag
    30661 caaagaaggg cgctttattg gtatcaatgc gccgcgttct ggcgcttatg acaacggctt
    30721 ccaggtcgtg catatgggct acggtgtcga taaaaaagtg caggtgccgc aaaaactgta
    30781 tgaggcgggc gtgccaaccg tgctggtggg taaggtggca gatatcgtca gcaatcctta
    30841 tggcgtgagc tggcaaaacc tggtggatag ccagcggatt atggatatca ccctcgacga
    30901 atttaacacc catccgacgg cgtttatttg caccaatatc caggaaaccg acctcgctgg
    30961 tcatgcagaa gacgtcgcac gttatgccga acgtttgcag gtcgttgacc gtaaccttgc
    31021 ccggcttgtt gatgcgatgc agccagatga ttgcctggtc gtgatggcgg atcacggcaa
    31081 cgatccgacc attggtcaca gccaccatac ccgcgaagtg gtgccgctgc tggtttatca
    31141 gcaaggcctg gctgctacgc agctcggtgt gcgcaccacg ctttctgatg tgggagctac
    31201 cgtgtgtgaa tttttccgtg cgccaccgcc acaaaatggt cgctcttttc tttcctccct
    31261 ccggtttgca ggagacaccc tatgagtttt gatccgacgg gttacaccct ggcccatgag
    31321 catctgcata ttgatctctc cggctttaaa aacaacgtgg actgccgcct tgatcagtat
    31381 gcgttcattt gccaggagat gaacgacctg atgacccggg gtgtacgtaa tgtgattgag
    31441 atgaccaacc gttacatggg gcgcaatgcg caatttatgc tcggtgtaat gcgcgaaacc
    31501 gggatcaacg tggtggcctg tactggttat taccaggatg cgtttttccc ggaacatgtg
    31561 gtgacccgca gcgtgcagga actggcgcag gagatggtcg atgaaattga acagggtatc
    31621 gatggcacgg agctgaaagc cgggctcatc gcggagatcg gctccagcga aggaaagatt
    31681 acgccgctgg aagagaaggt atttattgcc gctgcgctgg cgcataacca gaccggacgc
    31741 ccgatctcca cgcatacgtc gttcagcacc atggggctgg aacaactggc tctgttgcaa
    31801 gcccacggag ttgatctttc gcgcgtcacc gttggtcact gcgatctgaa agacaacctc
    31861 gacaacattt tgaagatgat cgatctcggc gcgtacgtgc agttcgacac cattggtaag
    31921 aacagttact acccggacga aaagcgcatt gcgatgctcc acgcgctacg tgaccgtggg
    31981 ttgctgaacc gcgtcatgct gtcgatggat attacgcgcc gctcccattt aaaagccaat
    32041 ggtggttatg gctatgacta tttattgacc acctttattc cgcaattgcg ccagtcagga
    32101 ttcagtcagg ccgatgtgga tgtgatgtta cgtgaaactc cctctcaatt tttccaataa
    32161 ggacagactc atgaaaaaga ttggcgttgc aggcttacag cgtgagcaga ttaaaaaaac
    32221 cattgaagcg acggctcctg gcagttttga agttttcatt cacaacgaca tggaagcggc
    32281 aatgaaagtg aaatccgggc aactggatta ctacatcggt gcgtgtaata ccggtgcggg
    32341 cgcggcattg tcgattgcca tcgcggtgat tggctataac aaaagttgca ccattgccaa
    32401 accaggcatt aaagcgaaag acgagcatat cgccaaaatg atcgctgaag gaaaagtggc
    32461 gtttggcctt tccgttgagc acgtcgaaca cgcgattccg atgctgatta accatctgaa
    32521 ataaaaggca cgactatgga tctgtatatt cagattatcg tggtggcgtg cctgacgggt
    32581 atgacatcgc ttctggcgca tcgctcggcg gctgtttttc atgacggcat ccgcccgatc
    32641 ctgccgcaac tgattgaagg ctatatgaac cgtcgcgagg cggggagtat cgcttttggt
    32701 ctgagcattg gttttgtggc ctcggtgggg atctctttta ccctgaaaac cgggctgctc
    32761 aacgcatggt tactctttct tcctaccgat atcctcggcg tactggcgat aaacagcctg
    32821 atggcgtttg gtcttggcgc tatctggggc gtgttgatcc ttacttgcct gttgccagta
    32881 aaccagctgc tgaccgcgct gccggtggat gtattaggta gcctcgggga attaagctcg
    32941 ccggtagttt ctgcttttgc actgttcccg ctggtggcga ttttctacca gtttggctgg
    33001 aagcaaagtc tggtcgccgc cgtggttgtt ctgatgaccc gtgtggtagt cgtgcgctat
    33061 ttcccacatc ttaaccctga atccatcgaa atctttattg gcatggtgat gctgctgggg
    33121 atcgcgataa ctcacgacct gcgtcatcgt gatgaaaatg atattgatgc cagcgggctt
    33181 tcggtgtttg aagaacgcac gtcgcggatt atcaaaaact taccctatat cgccatcgtg
    33241 ggagcattga ttgccgccgt tgccagcatg aagattttcg ccggcagtga agtgtcgatc
    33301 ttcactctgg agaaagcgta ctccgcaggc gtaacgccgg aacaatcgca aacgctgatc
    33361 aaccaggcgg cactggcaga atttatgcgc gggctgggtt ttgtaccgtt gattgccacc
    33421 accgccttag caacgggtgt gtatgcagtt gcgggcttta cctttgttta tgcggtgggc
    33481 tatctctcgc cgaatccgat ggttgcagcg gtattaggcg cagtggttat ttcggcggaa
    33541 gtcttgctgc ttcgttctat cggcaaatgg ctgggacgct acccgtcggt gcgtaatgcg
    33601 tcggataaca tccgtaacgc catgaatatg ctgatggaag tggcgctgct ggtcggttcg
    33661 attttcgcag caattaagat ggcgggttat accggattct ctatcgcggt tgccatttac
    33721 ttcctcaacg aatccctggg ccgtccggta cagaaaatgg cggcaccggt cgtggcagta
    33781 atgatcaccg gtattctgct gaatgttctt tactggcttg gcctgttcgt tccggcttaa
    33841 ggaaggcacc tatgaagacg tttcctctgc aaagcctgac gcttattgag gcgcagcaaa
    33901 agcagtttgc gctggtggac acgatttgcc gtcactttcc cggcgcggag tttctaacca
    33961 gcggtgattt gggcttaacg ccggggctga atcaaccgcg tattacccaa cgggtggagc
    34021 aggtgctggc tgatgcattt cacgcacagg ctgcggcgct ggtgcagggc gcggggactg
    34081 gcgcgattcg cgccgggctg gcagctttgc tcaaaccggg gcagcgtctt ctggtgcatg
    34141 acgcgcctgt ttacccgacg acacgggtta ttattgagca gatggggctg acgcttatta
    34201 ctgttgattt caatgacctg tcggcactga agcaggtcgt cgacgagcaa caaccggatg
    34261 cggcgctggt gcagcatacg cgccagcagc cgcaggacag ctacgtgctg gcagatgtgc
    34321 tggcaacgtt gcgcgcggca ggtgttccag cgttaaccga tgacaactat gcggtgatga
    34381 aggtggcgcg aatcggctgt gaatgcggcg cgaatgtctc gacattttcc tgcttcaagc
    34441 tatttgggcc agagggtgtt ggtgcagtgg tcggcgatgc tgatgttatc aaccgtattc
    34501 gcgccacgct ttactccggc ggtagccaga tccagggcgc acaggcgctg gaagtattgc
    34561 gttgtctggt gtttgcgcca gtgatgcacg cggtgcaggc aggggtatct gaacggttgc
    34621 tggctttgct taacggtggt gaggtgccgg aagtgaaaag cgcggtgatt gctaatgcgc
    34681 agtcgaaggt gttgattgtc gagtttcatc agccgattgc cgccagagtg ctggaagagg
    34741 cgcaaaagcg cggtgccttg ccttacccgg tgggtgcaga gtcgaaatat gaaatcccgc
    34801 cgctctttta tcgcctttcc ggaacgtttc gccaggcgaa tccacaatca gaacattgtg
    34861 cgattcgcat taacccgaat cgcagcggtg aagagacggt gctgcggatt ttgcgtgaga
    34921 gtattgccag tatttaatgc tggatagcac cgtcaagcta aattccgtac tgaacggtcc
    34981 cctcgcccct ttggggagag ggttagggtg aggggaacgt acgggatgta aaattcaacg
    35041 cgccagcatc gccggatgcg atgctggcgc atcttatccg gcctacggtt aatgtctttt
    35101 attatcaata gttaaggtga tgcgattggc gcggcagaag agttcggaga cgtgcaccgg
    35161 tttgtcattc tgatcaaagg cgattttgct gatgcgaaac aacggttcgc ccagttcgca
    35221 tttcagccat tttgcctgcg ggcgggtggc ggcaaagata tcgatggtct ttttgtcgct
    35281 gaccactcgc gtgtcaaaac gctcctgaaa taactgatag gtggagcttc cttcgacgta
    35341 aatctcatca aagtcaggat aacgcgacag cgggatccat gaactatcaa taaacagcgg
    35401 ctctttatcg agatacatca cccggcagag atggaacact tcgctgtttc cggggatgtt
    35461 cagctttttg caaaacggcg cggcgctgac ccgttcctgt tcgatcactt tctctttcgt
    35521 cgccttgcct tgtgagacgc caaaatcggt aaaaccactg acagtaagca gggcgttttc
    35581 aactttctgg ctttgtacaa aggtgccttt tccctgccag cggatcagta cgccgtccgc
    35641 cactaagtcg ctgatggctt tgcgaatggt aatgcggctg acgttatatt gtgtacaaag
    35701 ctcgttttcg gtagggatct gttgcccggc ctggtaaacc ccctgcgcga tatcatccag
    35761 cagtcgctgg cggacggtag cgtagaggag ttgatgagag tagcggtccg tagctgacat
    35821 aatcgaccct gaaataaaga atgaattact cggattatag ggtcgttttc cggcaagtaa
    35881 cagcacgttg gcgtgaagat gctcacgcca acgttatacc taccaggcac cgtggtactg
    35941 aatggttttc gccgcgcacg ccgttccctg cgccatcgcc tgcggtaatg tcatccccgc
    36001 agaccagccg caaaggaatc cggcaatgaa cgaatctccg gcacccatgg tgtcgataac
    36061 cgtcaccggt tcaggagcct gacgccagaa ctgcgcgcca tcccaggcaa tgctgccgtt
    36121 ttcacccagc gtgacaatca ctgttcctgc gccacgggca acaatcgctt tcatcttcag
    36181 acgcagcgtt tcgtcttctt gcggtgcgga ggcaaaggca aaatcgagat gcggcaccag
    36241 tgtctgccag agcgggctgt cccacttgtc ggagaagtcg aaagcggtaa gtttgcccgc
    36301 agcgtgcagc tgtgggaatg cgtcttccgc atgtccccag attgccgcgt gcacaatgtc
    36361 atactgcgcc agccaggcgt aatcctcttc actcagggca aagtcggcca tcacgccttc
    36421 ggtgtagtcg ccaaaaacgc gatcattgtc gtgcagttcc acctgagttt gtgcggtaac
    36481 gccgtgtttc gtatggacat ggctgatatc gacgcccatg cgggcgagat cctgcttcag
    36541 ctttgtgccg tagtcatcgt cacccaccca ggtaatgcat cccggctgta tgccgtagcg
    36601 agtgcagtac accgccacat tgaccgcatt accgccagaa aacgctttat tcagttgcgg
    36661 gtagatatcg acgcagttat cgccgattgt cgccagggtt ttcatctctc atcctccggc
    36721 agcagcgcgc gaaagcgttc cagcgcctgg cgggcataga gtctgggctc gttcatatac
    36781 atcgtcacca gctccaccgt acagtaaccc tcatagcccc gctcaataat atcgcgcatc
    36841 agttcccgca gcggcatttt gccttctcca ggaatgtaat gcgtgtcgct ggccccgtcg
    36901 ctgtcgacaa tatgcagatg acgtaattta tcgcccagtt tgtcgaaata actcatcacc
    36961 ggttccgcct ggacatacgg cgcgcaaatg tcgaccatgc tgaacaggcg cggcgaaggc
    37021 accagcgcca gcgcatgaag cacatcatta gcattacaca cgatgttcga ttcatacggc
    37081 gttaacggtt cgaggattag atccatgccg atgttttccg cgtactcata cagttcgctt
    37141 aggttctctg ccagccgtcc ccagataaca ttaggtggcg tgagatagcc cgcgtgggcc
    37201 gcggaaatca gcgtataacc cgcgttcatc tcttttgcca tatccatcgc cagcttgatc
    37261 atgtcgaggc tttcgcgacg catatgttca tcgcccagca tcatgttata cggatagcca
    37321 ttggtttctg gcgtatagcc gataatcggc atctgatacg tctgcgccag cgccttgatt
    37381 tgtttgatgc cgcccgcttt taagtccggc gcgaacgcgt gcgggcgacc gccccaaatt
    37441 tcgatgccgt cgtaacccag ctcgcttgca tcacgaaatg cgtgttcaat aggcagccgc
    37501 tggtggccgc aggtaaacat acctgttttc atcgggtaac tccttgcgta aatccggccc
    37561 gttgccgggc cgggcgaggg attaatattc caccagacca ccgtaatagc ggcgttcatc
    37621 cgggttgtga tctttgtaaa tagacaggta gtagcagagc cactccattg gcacgaacat
    37681 caggaacggt gccagccacg ggtgcagccc ttgcgaaatt tcggcgtaat cgatgacgat
    37741 cacgttgtca gtacgctgtt taacaaagtt aatggcgcgt tcggtggtgt ggcgactttc
    37801 atcattgccg agcaggaaca ggaacggaac gcccggttcg acaatctcca gcgggccatg
    37861 gcggaactct ccgctctcaa tcacgcagcc gtgcgtccag gtaaattcca tcagcgttac
    37921 aatgccttct ttgtaaccca gcggacgcag cggacccgca gcaacggtat aaatcatcgg
    37981 ccactggctg gccagttcac caagctggcg gcctttttct tcccaggtgc gtaccagatg
    38041 accgagcgca ttcggcaact gcttgagatc gtttttgatc ttgccgattt ccgcgttcgg
    38101 cgcgaggcgg gtgatcatct ccagcaccac gctgtagcag agcagcaggt gaatttccca
    38161 gatacagtcg gcctgataat caatgctaaa ttccgccgcc gaggtaatcg ggctgtccgc
    38221 gcgtttggtg aacgccgcag tgagtgcgcc gcaggcccgg cccagctcca gcgctttgat
    38281 tacctcttcc gttttaccgt agtcagaaac gccaatcacc gcgcaacgat cgtcgaggcg
    38341 atacggggtg ttatcgcaga actcccagcc ggaaatggcg tagacctgaa gatcggaaaa
    38401 gcgatccgcc agatgtttcg ccgcttgcgc cgcgttgagt ggcgagccgc aggcaacgaa
    38461 ataaatacga tcaatgtcgc gcttcaccat ctcttccacg atggcgtgca ccagcggaac
    38521 gtcatggctg agaacttttt ccacttcctg aaccatattt tcggtgacca gaaagtccac
    38581 ggtgctttta tcaatatcca acattttatt ttctccagat aacattacga caaagcgttg
    38641 agctggcggc tacgcttcgc ccagaaggcg taagcaggca gaccagtagc aataacgatg
    38701 acggcgcaga taaggccggg aatcggtgcc cagacaaagg ttgaggcgac cagaatgagg
    38761 cttgacgcaa tggcgagggt ggtcatcagc ccgaaagcag gagtacgcca cagcggtttg
    38821 taatcgtcgc gtttacgaca ccagatgatg gagccgaagg tgagggtatt tttgaaacac
    38881 atcaccaggg tgaaataacc cagcaggctg gtgagatcgg aaacgaagat gaagaagagc
    38941 cccagcgccc cttgcaggat gatggagaca tccggcgtgt tgtatttcgg atgcacatgg
    39001 ccgaagcatt taaagaacag gttgtctttc gccatcgcgt attccagccg cggctggtac
    39061 atcacgcagc tggaaagcga accaagaatg acgatcatcg ccgtgatggc aacaaagatc
    39121 ccagcggtgc tgccgagtgc ggggatccag gtcagggcgt cggaaatcgg cgtttcagaa
    39181 ttggcgagtt tgtcgaaggg catcaggtcg gaaatcacca gcgccagcag ggtgtagagc
    39241 accagaacca gcagacagga accaatcagc gctcgtggca tggtttttcc ggggttttta
    39301 atttcgccgg tcatataaca gatagaggcc atgccggtat acgaccaact ggtggcagag
    39361 atccccgcca gcagcgccat aaagctgccc gttgcgccaa tcgcagtggt ggtaggggcg
    39421 gcaaaattct ccgctttaaa ccagaagatc ccaaggccaa tgacgatagt gaacgggata
    39481 attttggcga tggtaattag cgtctgaaac gctgcgccgc cttcaacgga gcgcaggtgt
    39541 agcaacataa aggcgataat taatccggcg gcgataaatt taccgggcaa cggatcgata
    39601 ggcgttaaaa agccaagatt gctgacaatc gccagcgcca taatcgacaa tgacggcgca
    39661 tcgttggccc agaagctggc ccagccggag aggaaagcca gcggtcggct tccggcattt
    39721 ttcagataaa cataatctgc gccattttcc ggataagcgg tggatagttc cgcatagacg
    39781 cacatttgcg ggatcacaat taacccgcca atgacaaacg tgagcaccgt aagccacggc
    39841 gtgcccgctg cttttgccac ttcacccaca gatacaaaaa taccggagcc gacggttgtc
    39901 ccgacggcga ttgcaagaac ggcccaaaat ccgagcttgc gttggagttc ctggcttccc
    39961 atagcgatac ctttttcttg tccaacacgt tgtaggggag agtttgcagt catttaaaag
    40021 gtaacatgac aatgcatgat gaatataata acatatgatg ttatgtaatg cggaggaaga
    40081 tcacaaaatt tcgcacagga tcgcgctgtg gctaatgaat gtagttatcc aattgaaatt
    40141 aaagtgaaaa tatttttacg ggtggggagc aagaaggata tagaaacaaa tacgccctcg
    40201 gaaaatccag agggcgtcgg gcaattaaac cggtgttagc cgatttctgt cagagactta
    40261 ctgtgcagta ggaccgcaac cgccaatgat tttagaaata gaaattgccg ggtgcagcag
    40321 gtagtcgtaa gagcagttct tatcacggtt ttcaatatga ccagtacatg cagttaacgt
    40381 agccagtact gcacctacca gtgcaacttt aataaatttg ttcatt
//
LOCUS       DAJVUJ010000014        39395 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.014, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000014 DAJVUJ010000000
VERSION     DAJVUJ010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39395
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.014"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(13..870)
                     /gene="murI"
                     /locus_tag="OQQ48_000738"
     CDS             complement(13..870)
                     /gene="murI"
                     /locus_tag="OQQ48_000738"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCS1951601.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
     gene            complement(815..2663)
                     /gene="btuB"
                     /locus_tag="OQQ48_000739"
                     /pseudo
     CDS             complement(815..2663)
                     /gene="btuB"
                     /locus_tag="OQQ48_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
     regulatory      complement(2717..2907)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            3021..4121
                     /gene="trmA"
                     /locus_tag="OQQ48_000740"
     CDS             3021..4121
                     /gene="trmA"
                     /locus_tag="OQQ48_000740"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HCS1951602.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPCILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     gene            complement(4161..4520)
                     /locus_tag="OQQ48_000741"
     CDS             complement(4161..4520)
                     /locus_tag="OQQ48_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HCS1951603.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4520..5170)
                     /gene="fabR"
                     /locus_tag="OQQ48_000742"
     CDS             complement(4520..5170)
                     /gene="fabR"
                     /locus_tag="OQQ48_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HCS1951604.1"
                     /translation="MMGIRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            5491..6891
                     /gene="sthA"
                     /locus_tag="OQQ48_000743"
     CDS             5491..6891
                     /gene="sthA"
                     /locus_tag="OQQ48_000743"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HCS1951605.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLVLDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            complement(6874..7791)
                     /gene="oxyR"
                     /locus_tag="OQQ48_000744"
     CDS             complement(6874..7791)
                     /gene="oxyR"
                     /locus_tag="OQQ48_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HCS1951606.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(8058..9431)
                     /gene="argH"
                     /locus_tag="OQQ48_000745"
     CDS             complement(8058..9431)
                     /gene="argH"
                     /locus_tag="OQQ48_000745"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HCS1951607.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDAFDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLDELQKFSHVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLG"
     gene            complement(9492..10268)
                     /gene="argB"
                     /locus_tag="OQQ48_000746"
     CDS             complement(9492..10268)
                     /gene="argB"
                     /locus_tag="OQQ48_000746"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HCS1951608.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            complement(10276..11280)
                     /gene="argC"
                     /locus_tag="OQQ48_000747"
     CDS             complement(10276..11280)
                     /gene="argC"
                     /locus_tag="OQQ48_000747"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HCS1951609.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPAINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQVLQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            11434..12585
                     /gene="argE"
                     /locus_tag="OQQ48_000748"
     CDS             11434..12585
                     /gene="argE"
                     /locus_tag="OQQ48_000748"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HCS1951610.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASIGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDIDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVIERWPGRLTVDELHPPIPGHECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            12937..15588
                     /gene="ppc"
                     /locus_tag="OQQ48_000749"
     CDS             12937..15588
                     /gene="ppc"
                     /locus_tag="OQQ48_000749"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HCS1951611.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEAPPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYKSWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIR
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDVYRGYVRENKDFVPYL
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            15771..17504
                     /locus_tag="OQQ48_000750"
     CDS             15771..17504
                     /locus_tag="OQQ48_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HCS1951612.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            17719..18528
                     /locus_tag="OQQ48_000751"
     CDS             17719..18528
                     /locus_tag="OQQ48_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS1951613.1"
                     /translation="MYHDISYLLSRLINGPVPDLAYQVDFPRLEIVLEGEFVDTGAGA
                     TLVPGDVLYVAAGGWNFPQWKTPTTTFSVLFGKQQLGFSVVQWDGKQYQNLAKQHVAR
                     RGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQIQTASRSQALFEAI
                     RDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYLNHTRLEHAKTLLKG
                     YDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLTEKPTTPE"
     gene            complement(18515..18856)
                     /locus_tag="OQQ48_000752"
     CDS             complement(18515..18856)
                     /locus_tag="OQQ48_000752"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCS1951614.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(18858..19736)
                     /locus_tag="OQQ48_000753"
     CDS             complement(18858..19736)
                     /locus_tag="OQQ48_000753"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418387.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HCS1951615.1"
                     /translation="MTSSAGQRISCNVVETRLNDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLNIRQIHLLPFHQYGEPKYRLLGKTWSMKDVAAPSSADVAT
                     MREMAERAGFQVTVGG"
     gene            complement(19702..21999)
                     /locus_tag="OQQ48_000754"
     CDS             complement(19702..21999)
                     /locus_tag="OQQ48_000754"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCS1951616.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAVLAETMAANCT
                     DAQRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDEQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLWEAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(22050..22370)
                     /locus_tag="OQQ48_000755"
     CDS             complement(22050..22370)
                     /locus_tag="OQQ48_000755"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCS1951617.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEARESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(22385..23464)
                     /locus_tag="OQQ48_000756"
     CDS             complement(22385..23464)
                     /locus_tag="OQQ48_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HCS1951618.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVVI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            23773..26274
                     /gene="ptsP"
                     /locus_tag="OQQ48_000757"
     CDS             23773..26274
                     /gene="ptsP"
                     /locus_tag="OQQ48_000757"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCS1951619.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLRQHLLTGVSEGLSCAEAIVAS
                     ANHFCEEFARSSSSYLQERALNVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMTQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            26286..26948
                     /gene="fsa"
                     /locus_tag="OQQ48_000758"
     CDS             26286..26948
                     /gene="fsa"
                     /locus_tag="OQQ48_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCS1951620.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKMLKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            26959..28062
                     /gene="gldA"
                     /locus_tag="OQQ48_000759"
     CDS             26959..28062
                     /gene="gldA"
                     /locus_tag="OQQ48_000759"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HCS1951621.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAAFSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYATLLVADQYGQRFLQEWE"
     gene            28336..28953
                     /locus_tag="OQQ48_000760"
     CDS             28336..28953
                     /locus_tag="OQQ48_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HCS1951622.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGNTLFYDPAYVRLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWKLKRPDSNIDHRRVPNLETWFSRHNKTRPISKNAGDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(28980..29885)
                     /gene="yijE"
                     /locus_tag="OQQ48_000761"
     CDS             complement(28980..29885)
                     /gene="yijE"
                     /locus_tag="OQQ48_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HCS1951623.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMHPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYTRHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(29979..32159)
                     /gene="katG"
                     /locus_tag="OQQ48_000762"
     CDS             complement(29979..32159)
                     /gene="katG"
                     /locus_tag="OQQ48_000762"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HCS1951624.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     SSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(32488..33378)
                     /gene="metF"
                     /locus_tag="OQQ48_000763"
     CDS             complement(32488..33378)
                     /gene="metF"
                     /locus_tag="OQQ48_000763"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HCS1951625.1"
                     /translation="MSFFHASQRDALNQRLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(33727..36159)
                     /gene="metL"
                     /locus_tag="OQQ48_000764"
     CDS             complement(33727..36159)
                     /gene="metL"
                     /locus_tag="OQQ48_000764"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HCS1951626.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(36162..37322)
                     /gene="metB"
                     /locus_tag="OQQ48_000765"
     CDS             complement(36162..37322)
                     /gene="metB"
                     /locus_tag="OQQ48_000765"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HCS1951627.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWTNNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            37599..37916
                     /gene="metJ"
                     /locus_tag="OQQ48_000766"
     CDS             37599..37916
                     /gene="metJ"
                     /locus_tag="OQQ48_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HCS1951628.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            38100..38708
                     /locus_tag="OQQ48_000767"
     CDS             38100..38708
                     /locus_tag="OQQ48_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HCS1951629.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTVKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            38853..39395
                     /locus_tag="OQQ48_000768"
                     /pseudo
     CDS             38853..39395
                     /locus_tag="OQQ48_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT         9656 a        10897 c         9904 g         8938 t
ORIGIN      
        1 cattcaacca aatcagccta aaactgccag tttttcgagc gtttcgaagc cgtaacgctg
       61 taaaacgggc aataattgtt cagcttctgg cgtcattgcc atacaaaagg caatattcgc
      121 atcggcagat tttgcatccg gggcttcatg ttctaacaac caggccgttc ggcgagcaat
      181 cgctgcgcca gaatccacta gccgggttcc ctctggcagc acttgtaaca gttcttcttg
      241 tagtagaggg aaatgggtgc aacccaatac aacggtatct ggcggctctt tcattcttaa
      301 ccacgggcgt aggatacgtt ttagtgcatc cagagaaaca tcttcgccat gtagcttcgc
      361 ttcagccaac tcaaccatct ctgccgagcc cagcatttct atctggcatt cattagcgaa
      421 acgcgcgatc agctcatgag tataagaacg tttaactgtt ccgcgggttg ccagtaatcc
      481 gacaatgcca tttgccgtca gacgtgcagc aggtttaatc gccggcacga caccaacaac
      541 cgggaagtcg aacttttcgc gtaatgcagg aagtgaaacg gtactggcag tgttgcaagc
      601 gaccacagcc agcgcaaggg gataacgttc ttgcaccgcg gtgacaattg ccaccactcg
      661 ctcaacaata aacgcttcgc ttttttcgcc atacgggaaa gcgacgttat cgaaagcata
      721 aatgtaatgg agatccggta agagatgccg gatctcgtca tagaccgaca acccaccgac
      781 gccggagtca aacaccagca cggtgggacg tggttcagaa ggtgtagctg ccagacaagg
      841 tgtattcccg tcctgcagtt tggtagccat agactgtctc ataatctttg tcgaacaggt
      901 tggctatttt accacgaact gtcaggtgag aggtgaccgg atacgcaacc gcaagatccc
      961 acaagctcac accgcccatt ttaacggttt gataaggata agatgagtaa tccttatcat
     1021 agcgagtgcc taaatactga taagtaatac cccagtcgaa gtcatacaac tgccagtcga
     1081 gctggtattt cacctgctgt ttagcacggc gtaacaacgg cgtgtcggta atcgcattgc
     1141 gcgcatcgac ataatcataa ctcacagtat gcgtcagtgg tccggtatca aaattggcgg
     1201 tcgcctcgac gcccttaatc cgcgctttcc cttcgttgta atatttcagg gtgtgatcat
     1261 cataatcgat caagtcactg acatcgttac gatatccgga aatacgccag ttcaccccag
     1321 cggttaagcc ttcaaacgcg ccttcccact gtttgctttt ctccgggtcc agattcggat
     1381 ttccgtagaa gccatacagt tgccccagat ttggtgcctt ataagatgtc ccgtaggaag
     1441 caatgaagcg ataaccttcg atgaattccc aaccggcgct ggtttgccag gttccatgac
     1501 gaccaaactg tgagttatcg tcactgcgtg ctgcgccttc aaaggtaaaa tcgccgactt
     1561 gttgcagccc ggtcagatag atgccggtat tacgttgatc atatccatcc tcaacataac
     1621 ctgtacccgg cgtcgtagtc tgtttctgcc agtcgacacc cgcaccaata ctaccgtgac
     1681 caacgatgac attgtttgcc cactggacgg tgtattgctt catctcatcg agcgtcgccg
     1741 acgaatcata acgaccataa tggggatcgt agttgtaatc tttgctatgg ctatagctgg
     1801 taatgagttg tgatttaatc agttcgccgt tatagcgcag cccggcgtcc caactttggc
     1861 tatagagttt acggatatcg agcaacggtg aaccgggaga ataatacgcg tcataattgg
     1921 tacggttatc atagccatag ccgcgcacaa agccgctcca ggcatcagta aagttatgct
     1981 ccagcgcgcc ataaagcgtt ttacttaaaa aaacatcgtt atctgtctgc gcttgcgttc
     2041 cggtattacc ataggcaaca acatcataac catgagtatg ggcataatcg cccaacagcg
     2101 ttacccgtgt cttatccccc agttgttgct gcgtagagac atcatagttc tgataactat
     2161 tgcttcccca ccctgctgaa atttccgttc cgggttcatc gcgcgtcgtg atgatattca
     2221 ccaccccgcc tattgcatcg gaaccataaa cagcggagcg cggcccacgg atatattcaa
     2281 cacgctggac aagcgcacgc aatagggaac tggctaaggt cggcagaacc actcaccccc
     2341 gccagattca ggcgtacgcc atcaattaac accaacacat gactggcatt tgtaccgcga
     2401 ataaaaatag atgagagctg acctgaaccg ccgttttggg tgatatcgac gcccggaaga
     2461 cggcgcagca catcattgac cgaggtcgac tgccagcggt cgatatcctg acgggtcaca
     2521 acggtggttg gtgcaagcac agtgctgcgc ggctgttcaa aacggttagc agtaacgacg
     2581 agagtatccg ggctggtatc ctgtgcccaa gcggaaaagg ctgtgacaga acacgccgtc
     2641 agcagcgaag cttttttaat cattgtaaag catccacaat agaagaagga tgccgcaggt
     2701 ttcatcaata ttacgcgatg atgagacgtt ggccggcagg tcttcgggct tggaggggta
     2761 tctaagatac taagagatga tgacttccca ccggatggca gtgtccgcat aacgcaatca
     2821 tcgcaccttt ccttaccgct gcgcgtcagc tccagattcg cactggattc cctattaact
     2881 cacaggaccg gcaagtggat gctacaggtt gtaacaagtt actgtccaga cgtagctcac
     2941 aaataggaat tcatcaagat ctggacatct gatgagcaat ccctacaatc gccgcgtact
     3001 ttaatttttc aggatacatc atgacccccg aacaccttcc aacagaacag tatgaagcgc
     3061 agttagccga aaaagtggta cgtttgcaaa gtatgatggc accgttttct gacctggttc
     3121 cggaagtgtt tcgctcgccg gtcagtcatt accggatgcg tgcggagttc cgcatctggc
     3181 acgatggcga tgacctgtat cacatcattt tcgatcaaca aaccaaaagc cgtatccgcg
     3241 tggatagctt ccccgccgcc agtgaactta tcaaccagtt gatgacggcg atgattgcgg
     3301 gtgtgcgtaa taatcccgtt ctgcgccaca agttgttcca gattgattac ctcaccacgc
     3361 tgagtaatca ggcggtggtt tccctgctat accataagaa gctggatgat gagtggcgtc
     3421 aggaagcgga ggccctgcgc gatgcactgc gcgcgcagaa tctgaatgtg catctgattg
     3481 gtcgggcaac gaaaaccaaa atcgagctgg atcaagatta catcgacgag cgtctgccgg
     3541 tcgcagggaa agagatgatc taccgtcagg tagaaaacag ctttacccag ccgaacgcgg
     3601 cgatgaatat tcagatgctg gaatgggcgc tggacgtaac caaaggctca aaaggcgatt
     3661 tactggagct gtactgcggc aacggtaact tttcattagc gctggcgcgc aattttgatc
     3721 gggtattagc caccgaaatc gctaagccgt cggttgctgc tgcgcaatac aacatcgcag
     3781 ctaaccatat tgataacgta caaattattc gtatggcggc agaagaattt actcaggcga
     3841 tgaatggtgt gcgcgagttt aaccgcctgc aagggatcga cttaaagagt tatcagtgcg
     3901 aaaccatttt tgtcgaccca ccgcgcagcg gtctggacag tgaaaccgag aaaatggtgc
     3961 aggcgtatcc gtgtattttg tacatctcct gtaacccgga aacgttatgc aagaatctgg
     4021 aaacattaag ccagacgcac aaggtcgaac gtctggctct gtttgatcag ttcccctaca
     4081 cgcaccatat ggagtgcggc gtattactga ccgcgaagta aaacccatgc cggatgcgcc
     4141 agcatccggc ataataccga ttactcagca acttcctgct tacggttacg catcttcgcg
     4201 ccaatccaga acaccataat gacggagagt actgccggga agaagttaga gccgatatcc
     4261 ggatattccg cacgaactac cgtgctgtac agcagtacgc cgagaataaa acaggcagct
     4321 gccagacccg gcaagcctac cggcatagtg cgattaagat aacgttgatg caggcagtaa
     4381 accgtcagca ccagggaaat aatcgggaat acagaaaatg gcacaatgga gctaaacagc
     4441 gctgcgaaag taccattaat cgataagcca gcaactaacg ccagcagcag cgtacctcta
     4501 tcttgatttg cttgtttcat tactcgtcct tcacatttcc cggaataatt gcggttttct
     4561 cttgttcacg gcgataccag taataagccc ctttcgaaat cattcgcagt tgcagtacca
     4621 gtcgctcttc taattgccga cgttgttcga cgccgacgtc caacgcctcg gcacccgcac
     4681 tgaagacaat tgtcaccatt gcttcggctt gcgcttcagt aaacgcacgc ggcatatggt
     4741 tttcgagttc cagatagtcc gcaagttccg caatgaagtg ctgaatttca cgcgcaacgg
     4801 cggcacgaaa cgcagcggag gtgccggagc gttcccgcaa taataaccgg aaggcgttag
     4861 gattattacc gatgaactcc ataaatgtgg agaccgaggt gcggatcaca ctcccgcctt
     4921 tggcgatacg ctgacgcgcc tggcgcatga gttggcgtag cattaaaccg ctctcatcaa
     4981 ccatggtcag acccagttcg tctacgtcgc ggaaatgccg ataaaaagag gtgggagcaa
     5041 tgcccgcttc acgcgccact tcacgcaaac tcaggctggc gaagctgcgt tcagcactta
     5101 attggctaaa tgcggcttcc accagcgaac ggcgggtttt ttctttttgt tgcgctctta
     5161 tgcccatcac gatgtctgaa tccttgccaa aagtactgct ggcactatac cagagaatga
     5221 acatactgga ttgctccagt attgtgaatg aacggtaacg caataaaaca tatttactgg
     5281 ccggttcttt tacgtacagc ggaaacctgc cgcttaaacg gagagtatcg tcgataaaaa
     5341 tccaataaaa cgtcagggca aaagtaagaa acagacaaag caaaggccgc tcaggatata
     5401 gccagataaa tgacggggat caattggctt acccgcgata aaatgttacc attctgttgc
     5461 ttttatgtat aagaacaggt aagccctacc atgccacatt cctacgatta cgatgccata
     5521 gtaataggtt ccggccccgg cggcgaaggc gctgcaatgg gcctggttaa gcaaggtgcg
     5581 cgcgtcgcag ttatcgagcg ttatcaaaat gttggcggcg gttgcaccca ctggggcacc
     5641 atcccgtcga aagctctccg tcacgccgtc agccgcatta tagaattcaa tcaaaaccca
     5701 ctatacagcg accattcccg actgctccgc tcttcttttg ccgatatcct taaccatgcc
     5761 gataacgtga ttaatcaaca aacgcgcatg cgtcagggat tttacgaacg taatcactgt
     5821 gaaatattgc aggggaacgc tcgctttgtt gacgagcata cgttggtgct ggattgcccg
     5881 gacggcagcg tcgaaacact aaccgctgaa aaatttgtta ttgcctgcgg ctctcgtcca
     5941 tatcatccaa cagatgttga tttcacccat ccacgcattt acgacagcga ctcaattctt
     6001 agcatgcacc acgaaccgcg ccatgtactt atctatggtg ctggagtgat cggctgtgaa
     6061 tatgcgtcga tcttccgcgg tatggatgta aaagtggatc tgatcaacac ccgcgatcgc
     6121 ctgctggcat ttctcgatca agagatgtca gattctctct cctatcactt ctggaacagt
     6181 ggcgtagtga ttcgtcacaa cgaagagtac gagaagatcg aaggctgtga cgatggtgtg
     6241 atcatgcatt tgaagtcggg taaaaaactg aaagctgact gcctgctcta tgccaacggt
     6301 cgcaccggta ataccgattc gctggcgtta cagaacattg ggctggaaac tgacagccgc
     6361 ggacagctga aggtcaacag catgtatcag accgcacagc cgcacgttta cgcggtgggc
     6421 gacgtgattg gttatccgag cctggcatca gcggcctatg accaggggcg cattgccgcg
     6481 caggcgctgg taaaaggcga agccaccgca catctgattg aagatatccc taccggcatt
     6541 tacaccatcc cggaaatcag ctctgtgggc aaaaccgaac agcagctgac cgcaatgaaa
     6601 gtgccatatg aagtgggccg cgcccagttt aaacatctgg cacgcgcaca aatcgtcggc
     6661 atgaacgtgg gcacgctgaa aattttgttc catcgggaaa caaaagagat tctgggtatt
     6721 cactgctttg gcgagcgcgc tgccgaaatt attcatatcg gtcaggcgat tatggaacag
     6781 aaaggtggcg gcaacactat tgagtacttc gtcaacacca cctttaacta cccgacgatg
     6841 gcagaagcct atcgggtagc tgcgctaaat ggcttaaacc gcctgtttta acactttatc
     6901 gaaatggcca tccattcttg cgcggatggc ctctgccagc tgctcatagc ggctgcgcag
     6961 cggtgagcca ggacgataaa ccaggccaat cgtgcggcga ggttccggct taatacacgg
     7021 caaataaaca accccatcgc gtttgcgctc cggcggcaca gccagcgcag gcagtaaagt
     7081 gatcccgcta cctgccgcca ccatgttacg cagtgtctcc aggctggtcg cgcggaagtg
     7141 tgtatcttca tccgccccgg cttcaaagca gaaacccatt gcctgatcgc gcaaacagtg
     7201 accatcttcc agcatcagca gtttttcccc tgccagatcg gccatcggta cgcattcgcg
     7261 gttcgcccac gggtgatctt catagatagc cagcaacatt ggctcatcaa acaacggcac
     7321 ttcaatgaat gcttcgctct ctttcaccag cgcgaggatc acgcaatcga gtttgccgct
     7381 gtccagttgc gccagtaact ggtgggtctg tgcttcatgc agatacattt ccagctttgg
     7441 aaatgtctgg tgcagcatcg ggataatatg cggcagcagg tacggtccaa ccgtaggaat
     7501 caaaccaata tgcagcggtc cggacatcgt ctcgccctgc tggcttgcca tctctttaag
     7561 gactttcacc tcacgcagca cggtacgcgc ctgatccacc agcagcattc ccgcctgggt
     7621 gaacaacact ttacggctgg tccgctccag caacatcacg cccagctcat cttccagctt
     7681 acgaatttgg ccgctaagcg tcggctggct aacgtggcag gaatctgccg cacgccgaaa
     7741 atggcggtgt tcagccaatg ccaccaggta ctcaagatca cgaatattca ttatccatcc
     7801 tccatcgcca cgatagttca tggcgatagg tagaatagca atgaacgatt atccctatca
     7861 agcattctga ctgataattg ctcacagaaa cggagcggca cctcttttaa cccttgaagt
     7921 cactgcccgt ttcgagagtt tctcaactcg aataactaaa gccaacgtga acttttgcgg
     7981 atctccagga tccgcttttt tttgccataa aaaagcccgg cgataagcca ggctcaaatt
     8041 tgtacatata aatgttctta ccccaaccgc gcctgcgcaa aagcaatcgc ttgggccacc
     8101 tgctgcggtg agacgccgcc ttttgccgca cgcttgtcga ggcacgattg tagcgacaga
     8161 atcggataga catcttcgcc aatcacgtgg ctgaatttct gcaactcgtc gagcggcaga
     8221 tcttccagcg gtttgccctg acgaatggct tccaccaccg cttcacccac gatatggtgc
     8281 gcctcgcgga aaggtacgcc tttcgccacc aggtaatccg ccagttcggt ggcgttggcg
     8341 taaccctgct gagccgcttc ctggcaacgt ggacgtttca cctgaatgcc gtccagcacc
     8401 agcgccgcca tatgcaggca gtccagccag gtatcgaacg cgtcgaacag accttctttg
     8461 tcttcctgca tatctttgtt gtaagccagc ggcaaacctt tcagcgtcat catcatgccg
     8521 gttaacgcac cctgcacccg accgcattta ccgcgaatca gctccagcgc atccgggttt
     8581 ttcttctgcg gcattaatga tgaaccggaa gtcacgcggt cagaaagctc cacaaacccc
     8641 gcttcgccgg tgttaaagaa aatcagatct tcagcaaaac gcgacagatg caccatgccg
     8701 atagcggcag ccgacagcag ttccaacaca tggtcacggt cagaaacgct gtccagactg
     8761 ttacgggtcg ccgaagcaaa gcccagccag cctgctaact gttcacggtc gatttcatag
     8821 gccgttcccg ccagcgcgcc acagcctaat gggctgacat ccagacgctt aagcgcatcc
     8881 tgcaaacggc tttcatcacg cgccagcatc tcaacatagg ccaggcacca gtgcgcgaac
     8941 gtcaccggct gggcgcgttg caggtgagtg taacctggca ttaccgcgtc ctgattgttt
     9001 tgtgcggttt ccaccagcgc cgattgcagc tgccggttag ccgtcagtaa ctcgctaacg
     9061 gtatctttgc accacagttt caggtcagtc gctacctgat cattacggct acgcccggta
     9121 tgcagctttt tgcctaactg gcccactttg tcgatcagtt tgccttccac ccagctatgg
     9181 atatcttcgg cgtcgctttc aaggatttgt tgtggcctgg cgcgaacatc ttccagcaac
     9241 acgttcagcg cctcttccag ttgcgcctgc tcttctgcgg ttaacacgcc gaccgttacc
     9301 agggctttgg accaggccac agagccaaca atatcctgct ccgccagacg gtaatcaaag
     9361 cgcagtgagt cgttgaattg tttgaaccgt tgatctgctg cctgggtaaa acgcccgccc
     9421 caaagtgcca taactctgtt tccttatttt gaaattcaat gccggaaagc tgcgcctccg
     9481 gctaacaaaa cttaagctaa aatccgcgta cccatcggca taccgttaaa cagtgccgga
     9541 agctgctccg catgacgcca ggaggcgata tctaccggac ggcccagcgt gcgggccgca
     9601 tccagcgccg cgttcacttt cactatcatg ccgtcagtaa taatgccctg ctcaatcagt
     9661 tgttctgctt tcgcggcggt catttcggca atgcgttgcc ctttgccgtc gagaatgccg
     9721 ctgacgtcgg agagcaaaat cagatccgcg cccagcgttg ccgccagcgc cgttgccgcc
     9781 tggtcggcat tgacgttcat cagttgccct tcgtctgtta cgccaatgga gctgaccacc
     9841 ggcagataac cgttctccag caaggagttg ataagcttag gcgaacctgg ctgcgccagt
     9901 ccaacatgac ctaactcttc atcaagctgg gtcactttga cgctgtcgcc atcaccgaga
     9961 aacaaaccta cagccgcaat ctgatgtttc ttcgcccatg ccaacagggt tttatttgcc
    10021 gttcccgcca gtgctccggt gataatgtct atctgatcag caggcgtcac ccgcaggccg
    10081 tttttctttt tcaccggcag attcagccct ttcatcagct catccaccac gcaaccgccg
    10141 ccgtgcacaa tcaccagcgg acgctgatgt gactcacgat aattcaccag tgcgctaaac
    10201 agacgttcca gcgcctcttc actatccagc agtacgccgc ccagtttgat aattaatgga
    10261 ttcatcattg cacccttaaa taagagactg cgtttccgca tagccgaaac gaatattggc
    10321 gcactgtacc gcttgtgccg ccgcgccttt cagtaagttg tcttcggtcg ccacaataat
    10381 cagatgctcg ccctgaacgg caaacccgat atcgcaaaat ggcagcccaa cgacattttt
    10441 cagcgccgga acgcctttgt catacagccg caccagcggt ttatgggcat acgcctgctg
    10501 taacacttgc gcgacttgcg cctgggtcac acccgatttc aggcggcagg taatggtttc
    10561 gagaatgccg cgcgggaaat tgcccagatg tggggtgaag ataacgtcag caccgaggtg
    10621 tgtggcgatc tctggttgat ggcgatgagt aaagacgcca tacggttgca ggctaacttc
    10681 acaaaaacta tttgaaatcg ctgccttacg tcctgcaccg ctcacgccgc tggtggcgtt
    10741 aatcgccggc cactgattga ggtcaagaag atcggcatca atcaacggtt tcagcgccag
    10801 ctgtgccgcc gtcggataac agcctggcac cgcaatcaaa ttcgcttctt ttaatttatt
    10861 accgcaccac tccgccagac cgtaggctgc ctgttccagc agttccgggt attgatgggt
    10921 aaagccgtaa tatttttcat agaaggtggc gtcgttaaca cgaaacgcgc cggaaaggtc
    10981 gaacaccacg cagcccgctt caagaaactg cggcgctaaa tcgtggctaa cttcatgggc
    11041 ggtggcgaga aacactacgt ccacccctgg gctaaactcg ctgatatccg acatcggctg
    11101 caacggcagc tcaacgatgc cttttagctg cggatgcaaa tcggagatta actttcccgc
    11161 atcattgctt tgcgctgaaa cagtcaaagc ggttatgttc atatgcggat ggcgatttac
    11221 ataggtcact agctctgcgc cagcgtagcc gctggcaccc acaatcagcg tattcaacat
    11281 cggggctatt caccttctta tgtctggttg ccaggttaaa cgtaaaacat tcaccttacg
    11341 gctggtgggt tttattacgc tcaacgttag tgtattttta ttcataaata ctgcatgaat
    11401 attgatacta tcatgaccag aggtgtgtca acaatgaaaa acaaattacc gccatttatc
    11461 gagatttacc gcgctctgat tgccacacct tcaataagcg ctacggaaga ggcactcgat
    11521 caaagcaatg cagatttaat cactctgctg gcggactggt ttaaagattt gggcttcaat
    11581 gtggaagtgc agcctgttcc aggaactcgc aacaaattca atatgctggc aagtatcgga
    11641 cagggggctg gcggcttgtt gctggcgggg cataccgata cggtgccatt tgatgacggt
    11701 cgctggacgc gcgatccgtt tacactgacg gagcatgacg gcaagcttta cggcttaggc
    11761 accgccgaca tgaaaggctt ttttgcgttt atccttgatg cgctacgcga tatcgacgtc
    11821 acgaaactga aaaaaccgct ctacattctg gcgactgctg atgaagaaac cagtatggcc
    11881 ggagcgcgtt attttgccga aactaccgcc ctgcgcccgg attgcgccat cattggcgaa
    11941 ccgacgtcac tacaaccggt acgcgcacat aaaggtcata tctctaacgc catccgtatt
    12001 cagggccagt cggggcactc cagcgatcca gcacgcggag ttaacgctat cgaactaatg
    12061 cacgacgcca tcgggcatat tttgcaattg cgcgataacc tgaaagaacg ttatcactac
    12121 gaagcattta ccgtgccata ccctacgctc aacctcgggc atattcacgg tggcgacgct
    12181 tctaaccgta tttgcgcttg ctgtgagttg catatggata ttcgtccgct gcctggcatg
    12241 acactcaatg aacttaatgg tttgctcaac gatgcattgg ctccggtgat cgaacgctgg
    12301 ccgggtcgtc tgacggtcga cgagctgcat ccgccgatcc ctggccatga atgcccaccg
    12361 aatcatcaac tggttgaagt ggttgagaaa ttgctcggag caaaaaccga agtggtgaat
    12421 tactgtaccg aagcgccgtt tattcaaacg ttatgcccga cgctggtgct cggtccgggt
    12481 tccatcaatc aagctcatca gcctgatgaa tatctggaga cgcggtttat taaacccacc
    12541 cgtgagttga ttatccaggt aattcaccat ttttgctggc attaaaacgt aggccggata
    12601 aggcgctcgc gccgcatccg gcaccgttgc caaactccgg tgccgcaata atgtcggatg
    12661 cgatgcttgc gcatcttatc cgacctacac ctttggtgtt atgtatggcg attttttaac
    12721 atttccataa gttacgctta tttaaagcgt cgtgaattta atggcgtaaa ttcctgctat
    12781 ttattcgttt gctgaagcga tttcgcagca tttgacgtca ccgcttttac gtggctttat
    12841 aaaagacgac gaaaagcaaa gcccgagcat attcgcgcca atgcgacgtg aaggatacag
    12901 ggctatcaaa cgataagatg gggtgtctgg ggtaatatga acgaacaata ttccgcattg
    12961 cgtagtaatg tcagtatgct cggcaaagtg ctgggagaaa ccatcaagga tgcgttggga
    13021 gaacacattc ttgaacgcgt agaaactatc cgtaagttgt ccaaatcttc acgcgctggc
    13081 aatgatgcta accgccagga gttactcacc accttacaaa atttgtcgaa cgacgagctg
    13141 ctgcccgttg cgcgtgcgtt tagtcagttc ctgaacctgg ccaacaccgc cgagcaatac
    13201 cacagcattt cgccgaaagg cgaagctgcc agcaacccgg aagtgatcgc ccgcaccctg
    13261 cgtaaactga aaaaccagcc ggaactgagc gaagacacca tcaaaaaagc agtggaatcg
    13321 ctgtcgctgg aactggtcct cacggctcac cctaccgaaa ttacccgtcg tacactgatc
    13381 cacaaaatgg tggaagtgaa cgcctgctta aaacagctcg ataacaaaga tatcgctgac
    13441 tacgaacaca accagctgat gcgtcgcctg cgccagttaa tcgcccagtc atggcatacc
    13501 gatgaaatcc gtaagctgcg tccaagcccg gtagatgaag ccaaatgggg ctttgccgta
    13561 gtggaaaaca gcctgtggca aggcgtacca aattacctgc gcgaactgaa cgaacaactg
    13621 gaagagaacc tcggctacaa actgcccgtc gaatttgttc cggtccgttt tacttcgtgg
    13681 atgggcggcg accgcgacgg caacccgaac gtcactgccg atatcacccg ccacgtcctg
    13741 ctactcagcc gctggaaagc caccgatttg ttcctgaaag atattcaggt gctggtttct
    13801 gaactgtcga tggttgaagc gccccctgaa ctgctggcgc tggttggcga agaaggtgcc
    13861 gcagaaccgt atcgctatct gatgaaaaac ctgcgttctc gcctgatggc gacacaggca
    13921 tggctggaag cgcgcctgaa aggcgaagaa ctgccaaaac cagaaggcct gctgacacaa
    13981 aacgaagaac tgtgggaacc gctctacgct tgctaccagt cacttcaggc gtgtggcatg
    14041 ggtattatcg ccaacggcga tctgctcgac accctgcgcc gcgtgaaatg tttcggcgta
    14101 ccgctggtcc gtattgatat ccgtcaggag agcacccgcc ataccgaagc gctgggcgag
    14161 ctgacccgct acctcggtat cggcgactac aaaagctggt cagaggccga caaacaggcg
    14221 ttcctgatcc gcgaactgaa ctccaaacgt ccgcttctgc cgcgcaactg gcaaccaagc
    14281 gccgaaacgc gcgaagtgct cgatacctgc caggtgattg ccgaagcgcc gcaaggctcc
    14341 attgccgcct acgtgatctc gatggcgaaa acgccgtccg acgtactggc tgtccacctg
    14401 ctgctgaaag aagcgggtat cgggtttgcg atgccggttg ctccgctgtt tgaaaccctc
    14461 gatgacctga acaacgccaa cgatgtcatg acccagctgc tcaatatcga ctggtatcgc
    14521 ggcctgattc agggcaaaca gatggtgatg attggctatt ccgactcagc aaaagatgcg
    14581 ggcgtgatgg cagcgtcctg ggcgcaatat caggcacagg atgcattaat cagaacctgc
    14641 gaaaaagcgg gtattgagct gacgttgttc cacggtcgcg gcggttccat tggtcgcggc
    14701 ggtgcacctg cccatgcggc actgctgtca caaccgccag gaagcctgaa aggcggcctg
    14761 cgcgtaaccg aacagggcga gatgatccgc tttaaatatg gtctgccaga aatcaccgtc
    14821 agcagcctgt cgctttatac cggggcgatt ctggaagcca acctgctgcc accgccggag
    14881 ccgaaagaga gctggcgtcg cattatggat gaactgtcag tcatctcctg cgatgtctac
    14941 cgcggctacg tacgtgaaaa caaagatttt gtgccttact tgcgctccgc tacgccggaa
    15001 caagaactgg gcaaactgcc gttgggttca cgtccggcga aacgtcgccc aaccggcggc
    15061 gtcgagtcac tacgcgccat tccgtggatc ttcgcctgga cgcaaaaccg tctgatgctc
    15121 cccgcctggc tgggtgcagg tacggcgctg caaaaagtgg tcgaagacgg caaacagagc
    15181 gagctggagg ctatgtgccg cgattggcca ttcttctcga cgcgtctcgg catgctggag
    15241 atggtcttcg ccaaagcaga cctgtggctg gcggaatact atgaccaacg cctggtagac
    15301 aaagcactgt ggccgttagg taaagagtta cgcaatctgc aagaagaaga catcaaagtg
    15361 gtgctggcga ttgccaacga ttcccatctg atggccgatc tgccgtggat tgcagagtct
    15421 attcagctac ggaatattta caccgacccg ctgaacgtat tgcaggccga gttgctgcac
    15481 cgctcccgcc aggcagaaaa agaaggccag gaaccggatc ctcgcgtcga acaagcgtta
    15541 atggtcacta ttgccgggat tgcggcaggt atgcgtaata ccggctaatc ttcctcttct
    15601 gcaaaccctc gtgcttttgc gcgagggttt tctgaaatac ttctgttcta acaccctcgt
    15661 tttcaatata tttctgtctg cattttattc aaattctgaa tataccttca gatatcctta
    15721 aggaattgtc gttacattcg gcgatatttt tttcaagaca ggttcttact atgcattcca
    15781 cagaagtcca ggctaaaccc ctttttagct ggaaagccct gggttgggca ctgctctact
    15841 tttggttttt ctctactctg ctacaggcca ttatttacat cagtggttat agtggcacca
    15901 acggcattcg cgactcgctg ttattcagct cgctgtggtt gatcccggta ttcctctttc
    15961 cgaagcggat taaaattatt gccgcagtaa tcggcgtggt gctatgggcg gcctctctgg
    16021 cggcgctatg ctactacgtc atctacgggc aggagttctc gcagagcgtt ctgtttgtga
    16081 tgttcgaaac caacaccaac gaagccagcg agtatttaag ccagtatttc agcctgaaaa
    16141 ttgtgcttat cgcgctggcc tatacggcgg tggcagttct gctgtggaca cgcctgcgcc
    16201 cggtctatat tccaaagccg tggcgttatg ttgtctcttt tgccctgctt tatggcttga
    16261 ttctgcatcc gatcgccatg aatacgttta tcaaaaacaa gccgtttgag aaaacgttgg
    16321 ataacctggc ctcgcgtatg gagcctgccg caccgtggca attcctgacc ggctattatc
    16381 agtatcgtca gcaactaaac tcgctaacaa agttactgaa tgaaaataat gccttgccgc
    16441 cactggctaa tttcaaagat gaatcgggta acgaaccgcg cactttagtg ctggtgattg
    16501 gcgagtcgac ccagcgcgga cgcatgagtc tgtacggtta tccgcgtgaa accacgccgg
    16561 agctggatgc gctgcataaa accgatccga atctgaccgt gtttaataac gtagttacgt
    16621 ctcgtccgta caccattgaa atcctgcaac aggcgctgac ctttgccaat gaaaagaacc
    16681 cggatctgta tctgacgcag ccgtcgctga tgaacatgat gaaacaggcg ggttataaaa
    16741 ccttctggat caccaaccag cagacgatga ccgcccgcaa taccatgctg acggtatttt
    16801 cgcgccagac cgacaagcag tactacatga accagcaacg tacgcagagt gcgcgtgaat
    16861 acgacaccaa cgtgctgaag ccgttccagg atgtgctgaa tgaccctgcg ccgaagaaac
    16921 tgatcatcgt tcatctgctg ggtacgcata tcaaatacaa ataccgctac ccggaaaatc
    16981 agggcaagtt tgatggcaat accgatcatg ttccgccagg attaagcgca gaagagctgg
    17041 aatcatataa cgattatgac aacgctaact tgtataacga tcatgtggtt gccagcctga
    17101 ttaaagactt taaagcggca gacccgaacg gattcctggt ttacttctct gaccacggtg
    17161 aagaggttta cgacacgccg ccgcacaaaa ctcaggggcg taacgaagac aacccgacgc
    17221 gccacatgta caccattccg ttcctgctgt ggacgtcgga aaaatggcaa gcgactcatc
    17281 cccgtgattt ctcacaggat gtcgatcgta aatacagcct ggcggaactg atccacacct
    17341 ggtcagattt ggcgggctta tcttacgacg gttacgatcc aacccgttca gtggtgaatc
    17401 cgcagttcaa agaaactacc cgctggattg gtaacccgta caagaaaaac gcgctgatcg
    17461 attacgacac tctgccgtat ggcgaccagg taggtaatca gtaattatca gccaggcgct
    17521 atttgccgga tgcgacgctt gacgcgtctt atccggccta cagatcgcgg cggtaggctg
    17581 gataaggcgt ttacgccaca tccggcaata caactgccgc aatgtttaca tcgaactccc
    17641 ctcaggccac acttttcagg tggccttttt taataatgcc agttactgtt ttcccgtcta
    17701 catacctaag ggaataacat gtatcacgac atcagctatc tgctctcccg cctgatcaac
    17761 ggcccggtgc ccgatcttgc gtatcaggtc gattttccac ggctggaaat tgtgctggaa
    17821 ggtgagtttg ttgataccgg cgctggagca acgttagttc ccggcgatgt gctgtacgtt
    17881 gccgctggtg gctggaattt cccacaatgg aaaacaccca ctaccacctt tagcgtgctg
    17941 tttggcaaac agcaactcgg cttcagcgtc gtgcaatggg atggcaaaca atatcaaaat
    18001 ctggcgaagc aacacgtcgc ccgacgcggc ccacgtatag gttcttttct gctacaaacg
    18061 ctcaatgaaa tgcaaatgca gccgcaggag cagcaaacgg caaggcttat cgtagccagc
    18121 ctgcttagcc actgccgcga tttgctcggc agccaaatcc agaccgcctc acgcagccag
    18181 gcactatttg aagctattcg cgattatatc gacgaacgct atgcctccgc gcttacccgc
    18241 gaatctgtcg cacaggcatt ctatatttca ccaaactatc tctcgcacct gtttcaaaaa
    18301 acgggggcca ttggttttaa cgaatacctg aatcacacgc gactggagca cgctaagacg
    18361 ttattgaaag gctatgacct gaaggtaaaa gaagtggcgc acgcctgcgg ctttgtcgac
    18421 agcaactact tctgccgtct gtttcgtaaa aacaccgaac gctcgccgtc ggaatatcgc
    18481 cgacagtacc acagccaact gacagaaaaa ccgactactc cagaataaga tgggtttgct
    18541 gcggcgcaga aagcactttg cgcaccgcgc tcattacccg ctgcggctca cgcaggaatg
    18601 cgtagatgct gcattgcaca tagcggcaat gttcaaatcg ctcggcaccg gcaagctcga
    18661 tatccgtaat caacagagca acatccgccc gacggatatc ctcgtccgct aaacgattct
    18721 ccgttcccag cgcgccctga gtttcaatgc tgacgcccca cttctctaac tggcacaact
    18781 tttccagccg ttccgccgcc atataagtat gcgccacgcc gcttacgcag gcggttactg
    18841 ccaccaggta tgccatttta acctcccacg gtaacctgaa atccggcccg ttctgccatt
    18901 tcgcgcatcg ttgccacatc ggctgatgac ggcgcagcaa catccttcat cgaccatgtt
    18961 ttccccagca ggcggtattt cggctcgccg tactgatgaa acggcaacag atggatctgc
    19021 ctgatattca gcgggatcag cacgtccagc gcctgctgca tattctcccg gctgagtgtg
    19081 aaaccgggga tcagcggcaa acgcgggatc acgttgacac cctcactcac cagcaaacgc
    19141 aggttctcca gtacgcgtgg caggttcatc ttcaccacat cccgcgcctg agtcgcgtcc
    19201 ataattttta aatcgaacaa cacttcatcg cacaatttcg ccagcggtaa cagcttgctg
    19261 gctggtgcgt ctccggcagt ttcaatggca catgagacac cccacagccg cagtcgctgt
    19321 aaaaaacggg tagcaaactc cgcctgcatt aacacttcgc cgccagaaag cgtgacgccg
    19381 ccgccggacg tgcgaaaaaa aatgtcatct ttcatcactt cccgttccag agcgtcaagg
    19441 ctgatatcgc gaccaatccg ttcaaacgcc ccggaggggc attcatccgc atcacgcaaa
    19501 catttcgcac agtgcagaca tttcgcctct ctgcgtaccg tctggatttt gccggagatc
    19561 gactccggat tagcacacca cgggcaaaga tgcggacagc ctttaaaaaa gaccaccgta
    19621 cgaatgccct cgccgtcatt cagtgaataa cgctgaatgt tgaaaatgcg cgccacatca
    19681 ttgaggcgcg tttccacaac gttacagctg atgcgctgtc cggcggatga tgtcatcctg
    19741 gatctccttc gacagttcga caaagaaggc gctgtatccg gcaacgcgca ccaccagccc
    19801 ggcataatct tgtgggcgct gttgcgcttc ccacaacgtg tcggcgttca ccacgttaaa
    19861 ctgaatatgc tgcaacttaa gctgggtaaa cgcccgtaag aagtcggcca gtttgcgtaa
    19921 tcccgcttca ccttccaggg tcgccggagt gaatttcacg ttcagcaacg taccgttaga
    19981 cagcagcgta ttatcgagct tactgactga cttcagtacc gctgttggcc cttgttcgtc
    20041 ctggcccagc ataggtgaca agccgccgtc tgccagctgt tctccggcaa aacgaccgtc
    20101 tggcgtcgcg ccaaccaccg atcccaacgg gacgtgagca gaaacggtat acgatcccgg
    20161 cgtgaagtaa ccaccgcgcg ggttctggta tttttccact tctttgcagt agtggcgcaa
    20221 cagttcggcg ctaatgttat ccacctcgtc gatatcgtta ccatatttct caaagcggtt
    20281 aattaagcga gcgcggactt tttcgccttc tggcgttgca aagttggctt ttaataccga
    20341 cagcaattcg tcaaaactta aacgctgttg ctcaaaaacc atccctttca acgcatggag
    20401 agaatcgctc aggttggcga taccgatccc ctgtacgccg gagaagttat aacgcgcgcc
    20461 gccatcggta atatcgcgac ctttttccag acaatcgctg ataaaagacg agagcagcgg
    20521 tacaggtgcc cagtcgcgat ggccgatatc acaaatattg ctgccctcaa ccatcagggt
    20581 gatgtagtgg ctgatcttgg cgcggatctg ctccagtaaa ccttcataag tcagcgcagc
    20641 attgccttca ttttcatgca ggcagatttc catcactttc agcagattaa acatcgcgat
    20701 gtcatgcaag ccgtaggttc tgccgggaat agataattcc acacagccca ctacggaata
    20761 gtcgcgcgca tcttccagcg aaacgccacg gttgaggaac gctggtacca ccacttcatc
    20821 gttaaagatt tgcggaatac cggtgccgag gcgaatggtt tcggcggttt tcatcaggaa
    20881 tggcgtgtcg atcaaggcgt tagtgcgcac gccgaggttc ggttgcggta attgcacgct
    20941 ttgataggcg tcaaggcaaa ggaacgaaag cacgttcacc gcgctacgtc cgttctcggt
    21001 taacccgccg agcagtgcgg tatagccggt cgggaaacct gcgaaataac gcgcgctgct
    21061 ggtggagcgc aacagcacga tgtcgttgca tttcacccat aaagattcga gcagttcttt
    21121 caggaacgcc ggatcttcgc cctgggttaa agatgcctga tagaacggca acatatactg
    21181 gtcgaagcgc cctaaagaca gcgaactggc gttggattcg tattgcagaa tgatgttcat
    21241 gtaccagaat aactggcacg cctgccagaa cgtctgcggc ttatgttcgg cgttatggcg
    21301 agagatctcc gcaatagtca gcagttcttc gcgacgctgg gcatctgtgc agtttgccgc
    21361 catcgtttcc gccagtacag cgtaacgcaa aatgtgtttc tgcgaggctt ccagcagtaa
    21421 cagcgctgcc tgataaaagt gattctccgg ctgttgctga caatgttgct gcatctgtgc
    21481 caccaactcc cccagcccgt gattcaacag gcgtgggtaa tcaataataa tgtgcccctg
    21541 gcctttatcc gtctggttaa tgctgaaaat ctgcgtgctg gtcgcggctt ttacttcatc
    21601 cgtcatctgc ccgttgatga aatctttcat cgaacgtttt tcccagtacg ggaacaactc
    21661 ttcacgatag atacgtttat cttcttcgct gatagcaaag cggtcctgcg gacgcgtcgg
    21721 gaattgatcc agctctttca gcagccagta agggtccatt tccggcgaca taatcccggc
    21781 gcgcggtttt acggtgcggt taccggcaat cagttcttca tcacgaatcg aaatttcaac
    21841 atgttcaagg atatacgctg ttgctttcgc ccggcgcaat atcaccggtt cgccttcggt
    21901 ttgccgatgg ctggcggtat aaagcagcgc ccgctccagc gagatttcac gggtattggc
    21961 aaacagtgca gttttgaggc gagagatacg attcgtcatg aggaactcct gtgggaaagc
    22021 gaacccgccc ttgcgagcgg gagaagaact taagcggttt gcgccaggtg agcttcaatt
    22081 ttgctcataa tggcgtcagc acgttttact gcatcgctga tgttgacgcg cacgatggtc
    22141 ttgccagcaa aacgctcttc aaacttgatg ccgatatctt tggtcagaat caccatatca
    22201 gcgctcgcca catcttccgc agtcagttca ttttccagac caattgaccc ctgggtttct
    22261 actttcactt cccagccttt cgctttcgcg gcactttccc gcgcctctgc tgccatatag
    22321 gtatgggcaa cacctgaagg acatgcggtt actgcaataa ttttcgtcat ggctcgttcc
    22381 tcaattaatt aatttcaaaa tccaaatcca ggtcgtcttc tttttcatca gtcgaagacc
    22441 catttttacg cgccagactt ttcagcacgt tcacacaaac agccgtcacc actgctccga
    22501 cagccactgc tgcgatataa cccagcttgc cttcaaccac cggcagcaca atcagaccac
    22561 cccagcctgc atagcactgc gcaccgacca gcgccgcagt tactgcacca caaactgaac
    22621 cgaccatgat ggaaggaatg acacgcagcg gatcggcagc ggcgaaagga atcgcccctt
    22681 cagtaacgcc aacacagccc atcaccagtg ccgctttgcc ggtttcgcgc tcttctgcgg
    22741 agaaattttt acgaccaatc agcgtcgcca gccccatccc cagcggcggg atacaaatac
    22801 ccacggcagc gatgaccacc acggtataaa caccctgagc aacgcaaatc agcatgaagg
    22861 cataggctac tttgttaacc ggaccgccca tatcgaacgc cagcatcaga cccatgatca
    22921 ccgccagcat aacaatgctg ccctgctgca tcccctgaag ccactgagtc aggctgttgg
    22981 tcaacgcccc tacaggctcg cccaagcccc acatcatgat gcctgcggta atcagtgtgc
    23041 cgacgatagg aataatgaag ataggcatca ccgagcgcag aactttatgc accggaattt
    23101 tcttcaggta atgcaccacg atgccgccga taatcccggc gatcagtgca ccaaagaagc
    23161 ccgcaccaaa gctgttacca acccaggcac cgatagcgca cggagccagc gcagaacgct
    23221 ctgcaatgga gtaaccaatg taagcggcga ggaaaggcac catcagcgtc aaacctgcaa
    23281 cgccgatatc aaacagtttt ttcaaattcg gatcggctac ggcatccggc actgcgcctt
    23341 tgccatacaa catgacggaa accgccagca aaataccgcc cgataccacg aagggaatca
    23401 tgtgtgaaac gcccgtcatt aaatgctgac gggtattttt caggatctgc accaactcat
    23461 tcataagctg ctcccgggct tgcgaaagcc catgtccata tttttgatgg aggaaacaat
    23521 aggcaatcgc aggcggagca aacagtggat aaaaaggcca tttactggac ttttgtaata
    23581 tcagtacaaa aatgcgatcc gcctcataac ttgcgataaa ccggaagcat gccgcaaaac
    23641 ggcgtcttgt gagacgcgtc gcaaattgac ggttacatta catttgtcca gcttttggca
    23701 gatttgtcac atggcaggcc gttgccgtgc gctcttatcc tgtaaacaga aaaccgtacc
    23761 gggagagagg caatggccct gattgtggaa tttatttgtg agctacctaa cggcgtacat
    23821 gcgcgtccgg caagccacgt tgaaacgctg tgtaatactt tttcatcaca aattgagtgg
    23881 cataacctgc gcactgaccg caagggcaac gccaaaagcg cccttgcgct gattggcacc
    23941 gatacgctgg cgggcgataa ctgccagtta ctgatttccg gggccgacga acaggaagcg
    24001 caccagcgtt taagccaatg gctgcgcgat gaattccccc actgcgatgc gccgctggcg
    24061 gaagttaaat ctgacgaact ggaaccactg ccggtttcac tgaccaatct gaatccgcaa
    24121 attatccgcg cccgcaccgt gtgcagcggt agtgcaggcg gcattctgac gccgatctct
    24181 tctttagatc tcaatgcgct gagtaatctt cccgcagcca aagacgttga cgccgagcaa
    24241 tccgcactgg aaaatggcct gacgctggta ctgaaaaaca ttgagtttcg tctgctggat
    24301 agcgacggtg ctaccagcgc gatactggaa gctcaccgat ccctggctgg cgatacatct
    24361 ttacgtcagc acttactgac aggcgtcagc gagggattaa gctgtgccga agcgattgtc
    24421 gccagtgcga atcacttttg cgaagagttc gcccgttcca gcagcagcta cctgcaagaa
    24481 cgtgccctga acgtacgcga cgtctgcttc cagttactcc agcaaatcta cggtgagcaa
    24541 cgcttcccgg caccgggcaa actgacgcag cccgccattt gtatggctga tgaactgact
    24601 cccagccagt tcctcgaact ggataaaaat cacctcaaag gtctgttact gaaaagtggc
    24661 ggcaccacct cacataccgt aattcttgcc cgttcgttca acattccgac actggttggt
    24721 gtagatattg atgcccttac tccgtggcag catcaaacga tttatatcga cggcaacgcc
    24781 ggggcgattg tggttgagcc aggggaagcc gtggcccgtt attatcagca agaagcccgc
    24841 gtacaggacg cccttcgtga gcaacagcgt gtctggctga cccaacaagc ccgtaccgct
    24901 gacggtatcc gcattgaaat tgccgctaac atcgctcact ccgtggaagc gcaggccgca
    24961 ttcggcaatg gtgcggaagg cgttggtttg ttccgcactg aaatgctcta tatggatcgc
    25021 accagtgcac cgggcgaaag cgagctgtac aacatttttt gtcaggcgct ggagtctgcc
    25081 aacggacgca gcattattgt gcgcactatg gacattggcg gcgacaaacc cgttgattat
    25141 ctgaacattc cggcagaagc taacccgttc ctcggttatc gcgctgtgcg tatttatgaa
    25201 gaatacgcct cgctgttcac cacacaacta cggtcgatcc tgcgcgcctc cgctcacggc
    25261 agcctgaaaa tcatgatccc gatgatctcc tcaatggaag agatcttatg ggtgaaagaa
    25321 aaactggcgg aagccaaaca gcaactgcgt aacgaacaca ttccgtttga tgagaagatc
    25381 cagcttggca ttatgctgga agtaccgtcg gtgatgttca tcatcgatca atgctgcgaa
    25441 gagattgatt tctttagtat tggtagtaat gacctgacgc aatatctgct ggcagtggat
    25501 cgcgataacg ctaaggttac tcgtcactac aacagcctga atccggcatt cttgcgggcg
    25561 ctcgattacg ccgtgcaggc ggtgcatcgc cagggcaaat ggattggtct gtgcggtgag
    25621 ctgggagcga aaggttccgt gctgccgttg ctggtcggct tagggctgga tgaactcagc
    25681 atgagcgcac catcaattcc ggcggcgaaa gcgcggatga cgcaacttga tagccgtgag
    25741 tgccgcaagt tgctcaacca ggcaatggcc tgccgtactt cgctggaagt ggaacacctg
    25801 ctggcgcaat tccgcatgac ccaacaggac gcaccgctgg tcaccgccga gtgcatcacg
    25861 ctggaaagtg actggcgcag caaagaagaa gtgctcaaag gcatgaccga taacctgctg
    25921 ctggcgggcc gctgccgcta tccgcgtaaa ctggaagccg acttatgggc gcgcgaggcc
    25981 gttttctcta ccggtctggg ctttagtttt gccattccac acagcaaatc agaacacatt
    26041 gagcaatcca ccatcagcgt ggcgcgtctg caagcgccgg tgcgctgggg cgatgatgaa
    26101 gcgcaattca tcattatgtt aaccctgaac aaacacgctg cgggcgatca gcacatgcgc
    26161 attttctctc gcctcgctcg ccgcatcatg cacgaagaat tccgtaacgc gctggttaac
    26221 gccgcctctg ccgacgctat cgccagcctg ctgcaacatg aactggaact gtaaaaggaa
    26281 acatcatgga actgtatctg gacaccgcta acgtcgcaga agtcgaacgt ctggcacgca
    26341 tattccccat tgccggggtg acaactaacc cgagcattat cgctgccagc aaggagtcca
    26401 tctgggaagt tctgccgcgt ctgcaaaaag cgattggtga tgagggcatt ctgtttgctc
    26461 agactatgag tcgtgacgcg cagggtatgg tggaagaagc gaagcgcctg cgcgacgcca
    26521 ttccgggcat tgtggtgaaa atcccggtga cttccgaagg tctggccgcc attaagatgc
    26581 tgaaaaaaga gggtattact acgcttggca ccgcagtgta cagtgctgca caagggttac
    26641 tggccgcgct ggcaggggca aaatacgttg ctccgtatgt taaccgcgta gatgcccagg
    26701 gcggggacgg cattcgtacg gttcaggagc tgcaagcgct gttagaaatg cacgcaccag
    26761 aaagcatggt gctggcggcc agctttaaaa cgccgcgtca ggcgctggac tgtttactgg
    26821 caggatgtga atccatcacc ctgcccttag atgtagcgca acaaatgctc aacacccctg
    26881 cggtagagtc agctatagag aagttcgaac acgactggaa tgccgcattt ggcactactc
    26941 atctctaaag gagcaattat ggaccgcatt attcaatcac cgggtaaata catccagggc
    27001 gctgatgtga ttaatcgtct gggcgaatac ctgaagccgc tggcagaacg ctggttagtg
    27061 gtgggtgaca aatttgtttt aggttttgct caatccactg tcgagaaaag ctttaaagat
    27121 gctggactgg tagtagaaat tgcgccgttt ggcggtgaat gttcgcaaaa tgagatcgat
    27181 cgtctgcgtg gcatcgcgga gactgcgcag tgtggcgcaa ttctcggtat cggtggcgga
    27241 aaaaccctcg atactgccaa agcactggca catttcatgg gtgttccggt agcgatcgca
    27301 ccgactatcg cctctaccga tgcaccgtgc agcgcattgt ctgttatcta caccgatgag
    27361 ggtgagtttg accgctatct actgttgcca aataacccga atatggtcat tgtcgacacc
    27421 aaaatcgtcg ctggcgctcc tgcacgtctg ttagcggcgg gtatcggcga tgcgctggca
    27481 acctggtttg aagcacgcgc ctgttcccgt agcggcgcta ccactatggc gggcggcaag
    27541 tgcacccagg cggcgctggc actggcagaa ctgtgctaca acactctgct ggaagaaggc
    27601 gaaaaagcga tgcttgccgc cgaacagcat gtagtaaccc ctgcgctgga acgcgtgatt
    27661 gaagcgaaca cctatttaag cggtgttggt tttgaaagcg gcggactggc ggcggctcat
    27721 gcggtgcata acggcctgac cgctatcccg gacgcgcatc actattatca cggtgaaaaa
    27781 gtggcattcg gtacgctgac gcagctggta ctggaaaacg cgccggttga ggaaatcgaa
    27841 accgtagctg catttagcca tgcggtaggt ttgccaataa ctctcgctca actggatatt
    27901 aaagaagatg tcccggcgaa aatgcgaatt gtggcagaag cggcatgtgc agaaggtgaa
    27961 accatccaca acatgcctgg cggcgcgacg ccagatcagg tttacgccac tctgctggta
    28021 gccgaccagt acggtcagcg tttcctgcaa gagtgggaat aacctactcc aaactcccgg
    28081 cttgtccggg agtttgaacg caaaattgcc tgatgcgcta cgcttatcag gcctacgcaa
    28141 tctctgcaat atattgaatt tgcgtgcttt ttaggccaga taaggcgttc acgccgcatc
    28201 cggcactctg tgccagtaat ctgaaactcc tacgttgttc aggagttttt tatccgcttc
    28261 tggacatttt ctctacagaa caatcgttag cccggaaggt cgaatctgtg actcttattt
    28321 tcacggacga accagatgaa agcttcactg gcgctgctca gcctgctaac ggcatttacc
    28381 agccactcct taaaatctcc cgccgttcca ccaaccgtgg tacaaatcca ggccaatacc
    28441 aaccttgcca tcgcagacgg tgccagacag caaatcggca acacgctatt ttacgatccg
    28501 gcgtatgtgc ggcttaccta tcctggcggt gatgtcccgc aagaacgcgg tgtatgttcc
    28561 gatgtggtga tccgtgcatt gcgcagccag aaggtcgatt tgcagaaact ggttcacgaa
    28621 gatatggcga agaattttgc cgagtacccg caaaaatgga agttaaagcg cccggacagc
    28681 aacatcgatc accgtcgggt gcctaatctg gaaacctggt ttagccgcca caacaaaaca
    28741 cgccccatca gtaaaaatgc cggtgactat caagcgggcg atattgtctc ctggcgatta
    28801 gataacgggc tggcgcatat tggggtggtg tcagatggct tcgcccgcga cggtacgcca
    28861 ctggtgatac ataacattgg cgcaggcgcg caggaagaag atgtgctgtt tagctggcgg
    28921 atggtggggc attatcgtta ttttgttaaa taagcgatcg ccccacatga agaaaaaatt
    28981 cagatgcttt ttacactgac agcttctttt ttcttacggc tcaccagcgc cagtgccagc
    29041 acaatcagca caataccgct accttcaacg gcccccggat tctcaccgag cagccaccag
    29101 gaaaagagta cgccgcaaac gggaacggcc agcgtgctta agctggcaat actggcaggc
    29161 aagtttttca atacaaacaa ccataagctc cacgccagcg ccgtcgccag aatcgcactg
    29221 taggccagcg cccagaacac ggtgggctgc cagtcaattt cacgttgcgg caccagtaaa
    29281 gcgaccacac tcatcaccag cgccgcatag agcatctgcc aggatgttaa cgataataaa
    29341 tccacgcgcg gatgacgggt gtataggcgt ttagcaacaa tcgcgctcgc cccccaacta
    29401 acaccggaga ggattgccag catggcactt ttcatcgaag agaaatcgag ttgccacggc
    29461 tgcaacacca aaaataagcc gaaagcggca atcagaatcg cgaaatattg cccacgtcgc
    29521 aggcgttcac cgagaaacaa cgcggcgaaa atcaccaccc agaacggcat ggtatagctc
    29581 aggatcgcca ccttccccgc acctccgctg accaacgccc actgcgccag accaaccatc
    29641 ccgcaggttt gtaacagggc aatggctaag gtgtatttaa acggcgtcgg gtgcattccg
    29701 cgaccacgta ataaaaggac gatgaataaa acgagagcgc cgaaaatgca gcgtaaggcg
    29761 gtaaagtcga aggcaccgat gtaactggtg acttgcttca tgaaaatcca gctataactc
    29821 cagataagtg tgagcacaac caggccactg attgccagtg ggttgctctt tcctgcggca
    29881 gacatgagag atccagtgtg tagttttcct gtggacacta tacgccggta aagaaacttc
    29941 agcgactgcc tgaaagacag tcactgaaga aggtcagatt acagcaggtc gaaacggtcg
    30001 aggttcatca ctttcaccca tgccgctacg aagtctttaa caaacttctc gtgggcatcg
    30061 ctgctggcgt aaacttccgc taccgcacgc aggacagagt tagaaccaaa taccagatcc
    30121 gcacggctgg ctgtgtattt cacttcgccg gtttcgcggt cacgtccttc gaacagctct
    30181 ttcgattcgt cagtcgcttt ccactcgtaa cgcatatcca gcaagttcac gaagaagtca
    30241 ttgctcaata cgccaacgcg gtcagtgaag acgccgtttt tgctgctatc gaagttggca
    30301 cccagtacac gcatcccgcc caccagcgca gtcatttccg gcgcggtcag cgtcagttgc
    30361 tgtgctttgt cgatcagcag tgactcggtg gtggaaacgt ccagacgagc gcgatagtta
    30421 cggaaaccgt cagcaattgg ctccagcagc tcaaacatct caatgtcagt ctgatcctga
    30481 cgcgcatcaa cgcgacccgg cgcaaacggt acatgaatgc tcaaacctgc ggcgcttgcg
    30541 gctttctcaa caccaaccac accagccagc acgatgatat ccgccagcga ggctttacca
    30601 gactctttct ggattttctc cagaacaggc agagcacgaa cggctgcggc gttcacatcc
    30661 cagtcgcgct gcggcattaa tgccagacgc gcaccgttgg caccaccgcg tttgtcgcca
    30721 ccacggaagg tagaagcaga tgcccaggcc accgatacca gctcactaac agacagacca
    30781 gaatccgcaa tcgcgaattt cagatcgata atgtcctgct cggtcgggtt gtagatcggc
    30841 tgcggcagcg gatcttgcca gatcagatct tctttcggca cttccggccc gatgtagcga
    30901 gatttcggcc ccatatccct gtgcgtcagt ttgaaccagg cacgggcaaa ggcttcgttg
    30961 aacgcctgcg gatcgttgag gaaacgacga gagatcttct cgaactcagg atcaaaacgc
    31021 agcgtcaggt cggtcaccag cattgtcggt ttacgtttct tcgacggatc gaacggatcc
    31081 gggataattt ccggtgcgtc taccgcttcg aactggattg ctccagccgg actgcgggtc
    31141 tgtacccact catatttgaa caggttctcg aagaaatagt tgctccactg ggtcggcgtc
    31201 tgggtccaga caacttccag accagaggta atggcatctg cgccaacgcc gctgccgtaa
    31261 gtgctcgccc agcctaaacc ttgttcttca atcggtgcag cttctggatc aggacctaca
    31321 tttgatgtcg gaccggcacc gtgagtttta cccagcgtat gaccacccgc aatcagcgcc
    31381 acggtttctt cgtcgttcat gcccatgttg ccgaaggtcg cacggatagc tgctgccgca
    31441 gaaagcggtt cgccgctgtg atccgggcct tccgggttaa cgtaaatcag acccatctcg
    31501 gttgcaccca gcggtgcttt cgccagcgct tccggatgac ggtgagtcag ccaggctttt
    31561 tcatcacccc agttaacatc cagatccggt tcccagacgt cttcacgacc ggcaccaaaa
    31621 ccgaaggtac ggaagccgga gttttccagc gccacgttac ctgcgaggat aaacaggtcg
    31681 gcccaggaga ttttctgacc atatttctgt ttgattggcc acaacaggcg acgcgcttta
    31741 tcaaggctta cgttatccgg ccaggagttc agcggtgcaa aacgttgctg accacgaccc
    31801 gcgccgccgc gtccatcgat tgaacggtaa gtacctgcgc cgtgccaggc catacgaata
    31861 aacagaccgg cgtagctgcc ccagtcggct ggccaccacg gctgagattc tgtcagcagg
    31921 gctttcagat cttttttcag gccgtagtaa tctaatttgc tgaattcttt gcggtagtca
    31981 aagtcctcac ccagcgggtt agaacgatta gaatgttggt ttaacaggtc aacacggagt
    32041 tgatttggcc accagtcgcg agtggttgtg cccgcccccg cgctctggtc gtgaccgccc
    32101 tgatggaacg ggcatttgcc agtggctgtc gtgttatgga tatcgtctga cgtgctcatc
    32161 aatgtgctcc cctctacagt gttaccgtta cgatacacag cgttagagag aagttataat
    32221 tgaattaatc cacggatgcg atagttgaga tcttacatat tgttggttaa agagatgtag
    32281 gtcaaattga tcgtaattaa gcttttttga ttatgtccga attcggacaa atgctttata
    32341 aaaaagggtt ttagcttcat atccttcaag taatgaggga gaaaacaagg ctgacaacct
    32401 tattttcccg ccctcatttc gaggcagcat tttgtgctct gtttaaaatt tgtgatcact
    32461 gtgtgatttt cacaaaagcc acactattta taaaccaggt cgaacaccca gcgtatggca
    32521 aatcgcgtaa ctcatttcag cgcggttaag cgtatagaag tggaaatctt tcactccttc
    32581 acggcttaaa atcttcacca tatccatggc aatattcgcg cccaccagtt tgcgggtttc
    32641 ggcatcatca tccaggccgt cgaacatttg cgccatccac gccggaatac gcacgttggt
    32701 catatcggca aatttcttcg cctgtttaaa gttagatacc ggcaaaattc ccggaataat
    32761 ttccacatca atgcccgccg atacacagcg gtcacgaaaa cgcaggtagc tttcgacatc
    32821 gaagaagaac tgagtaatcg cgcggttggc tccggcatcg actttgcgtt tcagattaag
    32881 caaatccgcc tgagcgcttt ttgcttccgg gtgaacttcc ggatacgccg ccacggagat
    32941 atcgaaatct gccacttctt ttaatagcgt caccaggtca gaagcataca tttctggctt
    33001 accacttccc ggcggcagat cgccacgcag cgccacgata tgacgaatac cgttattcca
    33061 gtagtcgcgt gcaatggtgc gcaactcgtc gggcgtcgca tcaatgcaag taagatgcgg
    33121 tgccgcttcc agaccagtgc gatctttaat gcctttaata atgctgtgcg tacggtcgcg
    33181 ctcgccggag ttcgcgccat aggtcaccga tacaaacttc ggtttcaggc tgctaaggcg
    33241 atcgatggag ttccacaggg tctgctccat ttcactggta cgcggcggga aaaactcgaa
    33301 cgaaacgtta atctgcccct ggacttctgc caggcgctga ttcagggcat cccgctggct
    33361 ggcgtgaaaa aagctcatac cttacctcat caatcgcttg tagttatatg ttgtgttgtg
    33421 cacatctatc cgtttagacg tccagatgta aagatgaagg aaaagccgaa gggcgtcaac
    33481 cgaaaaatga ccaatgatgg tgaggaaaaa tcagggaaga tgaaaaaact tcaggatggt
    33541 aagaaaaaga aaatccggta tgcaaagcaa accggactgc aaatataaac taaccatgta
    33601 cttattgctt cttgccggat tcgtcgtaaa cgcttaatcc ggcctaatcc tatgataatt
    33661 caatatattt cattagaaac cataaacccg aaaacatgag taccgggcat tattaaattt
    33721 ctgaaattac aacaactgtg ccagccggtt gatatccgac tgaatcgccc cggcggtgac
    33781 gtcgcgcccc gcgccaggcc cacggatcac cagcgggttg tcgcgatacc agcggctttc
    33841 gatggcaaag acgttatcgc acggcagcag tgatgccagc ggatggtctt cacgcaccgc
    33901 ctccacgccc acacgcgctt tgccgttggc atcgaaacgc gccacgtagc gcagcaccag
    33961 ccccatttcg cgggccgctt ccagccgttg caccatctgc tcgttaagtt catcgccatt
    34021 ttcaaagaaa tggtcgatgc tgccgccttc gcaatgagca ggcaccagcg attccacacg
    34081 tacctggtcc ggttcaatgt tgtaacccgc ttcacgcgcc agaattacca gcttgcgcat
    34141 tacgtctttg ccggagaggt cgtcacgcgg atcaggctcg gttaagccct gctgccacgc
    34201 ctgatccacc agctcggtaa acggcacgct accgtcgaat tgcaggaaca gccaggagag
    34261 cgtgccggag aagatcccgc tgatcgacaa aatagtatcg ccgctgtcga tcagatcgcg
    34321 cacggtgtgg ttgatcggca agcccgcacc gacggtggca ttgtacagcc agtgacgccc
    34381 ggttttttcg aaggcgtcgt ggatctggcg atatttattg ctgtcgctgg ctcccgccag
    34441 tttgttggcg ctgataacgt ggaaaccgtg gctggcgaaa tcaagatact gatcagcaag
    34501 ctgctggctg gcggtaacgt ccagcaccac taaatcatca tacggatggg cgcgcatcca
    34561 caggaacaac gactcttcat cctgctcaac cgcttcatcg ttgaagaagg ctaacgcgcg
    34621 gctggcgtcc agcccgtcat agctcaacag gctgcggcgg ctgtccacca cacctgccag
    34681 cacaaactca aagccggtac gtgccgaaag cgtgctctgc tcacgggcga acagttccag
    34741 ccaacgggaa ccgatattgc ccttaccgaa caataccagg ccgatgcgtt tttctgcgcg
    34801 gaagacggac tgatgcagcc cctgaatcag gctttcggtc gggccggtgc gcagtactgc
    34861 caccaggctg atgccgtcat cggactgcca ggtaaattcg accggctggc ctttcagttg
    34921 ctgccagaag cggtggcaat gcagcgggtt acgggtgacg cctgcaccga ccatcgccac
    34981 cagcgccagc ccctgacgca ggcgcagttc gccaggtaat cccgcttcgt cgaggatttt
    35041 cagcgcactg tcggccactt ctgaggtgta gcaaaattgc agcaactggc gatcgttatg
    35101 tacgccaacc gccagcgggc gtacctgcgc gcgtttcagg atttggtcga tctctttatg
    35161 cgccagtttg aaatcctgac tggcgggcac ctgaaactca atcaaacaga catcatcgtg
    35221 gctggtgaca atacgcgcac cagtaccgga ggccagcacg cgttcaatgc gcgtggaacc
    35281 ttgatccggc gtgtagctac agcgcagttg caggtcgatt tcgctgccag aaaccggctg
    35341 taaagtacgg gcgtgaagaa cgggagccgc caggcgcgcc agttcgctgg cctcatccag
    35401 acgcagcaac ggcagcaggc aggcatcttt cactttacgc gggtcggcac tgtatacccc
    35461 ggcgacgtcg ctccagatgg ttacgcgaga aacacccgcc agcgcaccga tttgtgtcgc
    35521 ggaatagtcg gaaccgttac gccccagcag caccgtttca ccggcgttgt tgcggctgat
    35581 aaatccggtc accaccagac gtttgcccgg atgttgcacc agcagctgtt gcagcaacgg
    35641 gtaagaaagc ccttcatcaa cctgcggttg tgcggcgcgt tcagcgcgta aaaactcgcg
    35701 ggcatcaagc caggccgctg gcagcccttg ttgattaagt accgcagaca tcaggcgtgc
    35761 cgaccatact tccccgtggc ccaccacttc cgcatacact gcgtcgttaa taccgctgtc
    35821 gagcagcgcc gccaggcgct caaggtcgct gacaaaagcg ctaatgaggc tatcagcttc
    35881 ttcagcgggt agcagaccgc taatcagatc gcactgatag cgacgcagcg tttgttgaac
    35941 ctgatgcgca gagagacgat cggtctggct tagtttcaac cagttaatca actggttagt
    36001 ggtgctaccg gcggcggaaa ccaccatcat atcgtcaggc tgagagtact ccgccataat
    36061 gcccgcaaca cgcaaataac acttcacatc agccagacta ctgccaccaa atttatgcag
    36121 ttgacgacct ttcgcccctg cctgcgcaat cacactcatt tttacccctt gtttgcagcc
    36181 cggaagccat tttccaggtc ggcaattaaa tcttcgccat cttcaatacc ggtggagata
    36241 cgcagcagcg tctcggagat cccggcggca gcacgcgctt ctggtgccat gcctgcatgt
    36301 gtcatggttg cggcgtgaga gattaaactt tccactcccc ctaatgattc cgccagcgta
    36361 aacaacgaca gcccgcccag gaaacgacgc agcgtctgct catcgccatc cagttcaaaa
    36421 ctcaacattg cgccaaagcc tttttgctgg cgcgcggcaa tttcatgccc ctgattttcc
    36481 ggcagcgacg ggtgatacag ttttttcacc aacggctggg tttgcagata tttcacaatc
    36541 gcctgcgcgt tgcgctgggc cagctccata cgcggcacca gcgttcgcaa cccgcgtagc
    36601 agcagatagc tgtcaaacgc gccgcccgtc acgccaatat tgtttgtcca ccaggcaagt
    36661 tcagtgacaa cgtccgggtc tttagcaatc accacgccag ccaccacgtc agagtgaccg
    36721 ttcagatatt tcgtacatga atgcaacacc agatcggcac ccaatgccag cggattttgt
    36781 aatgccgggc ttaagaaggt gttatccacc acgctcaccg ccccgacttc ccttgccaga
    36841 tggcagattt tcgcaatatc cacgacgcgt aacaatggat tacttgggct ttctaccagt
    36901 accagtttag gtttttctgc cagcgctgcc cgtaatgcct gttcatcgcc ttgatcaaca
    36961 aacaacacgc gatagcaacc gcgtttcgcc agactgtcga acaggcgata gctaccgccg
    37021 tagcagtcgt gcggcgcaac cagcagatcg ccaggtttca aaaagacggt cgttaccaag
    37081 tgaatcgcgg acatgccggt attagtaagt actgcgccag caccgccttc cagttctgcc
    37141 agcgcacgct gaaccacatc gcgcgttggg ttgccgcgac gcgagtaatc atgcgcgcgc
    37201 ggttcattaa atccggtaaa gttataggtg ctggaaagat ggatgggagg gacaacgcaa
    37261 ccatactgtt cgtcgtcatt taacccgcta cgcactgcga tggtggcctg tttacgcgtc
    37321 atgtgatgaa gtttcctggg ctttgtcggt gaaatgtcag gcaccagagt aaacattgtg
    37381 ttaatggacg tcaatacatc tggacatcta aacttcttta cgtatagatt gagcaaatcc
    37441 caaatagccg ttaaaattat atgcattatc acgccgacag gtgcattaca cgatgtcacg
    37501 gtaacgcctg tacggtaaac tatgcgggtt tacggtcagt acccacataa attgtgtggt
    37561 ctggtctcaa tttattgacg aagaggatta agtatctcat ggctgaatgg agcggcgaat
    37621 atatcagccc atacgctgag cacggcaaga agagtgaaca agtcaaaaag attacggttt
    37681 ccattcctct taaggtgtta aaaatcctca ccgatgaacg cacgcgtcgt caggtgaaca
    37741 acctgcgtca cgctaccaac agcgagctgc tgtgcgaagc gtttctgcat gcctttaccg
    37801 ggcaaccttt gccggatgat gccgatctgc gtaaagagcg cagcgacgaa atcccggaag
    37861 cggcaaaaga gatcatgcgt gagatgggga ttaacccgga gacgtgggaa tactaaagca
    37921 aaaaagagcg gcgcggagtg gaatcgcctg atgcgctacg cttatcaggc ctacgtcata
    37981 ttgcaattta ttgaatttgc acgaacttgt aggccggata aggcgttcac gccgcatccg
    38041 gcataaacaa cgagcacgtt gtctgcgacc caccgctttt tatacatgga cgtttaacta
    38101 tgaaaaacag gctgctgatc ctcagcctgc tggtttctgt acctgccttt gcctggcagc
    38161 cacaaaccgg cgacatcatc tttcagatct ctcgctcatc gcaaagtaaa gcgatccaac
    38221 tggcgaccca ttccgattat agccacaccg gtatgctggt gatacgcaac aaaaagccct
    38281 acgtttttga agcggtcggc ccggtgaaat acaccccgct caagcagtgg atcgcccatg
    38341 gtgaaaaggg caaatacgtt gttcgccgcg ttgaaggcgg actgagtgtt gaacaacagc
    38401 aaaaactggc gcaaacggta aaacgttatc tcggtaaacc gtatgatttc agcttctcat
    38461 ggagcgacga tcgccagtac tgttcggaag tggtgtggaa ggtttaccag aacgcgctgg
    38521 gaatgcgcgt gggcgagcag cagaagttga aagagttcga tctcagcaac ccgctggtcc
    38581 aggctaagct caaagaacgc tacggcaaaa atattccgct tgaggaaacg gtagtttcac
    38641 cacaagccgt tttcgacgcg ccacaactca ccacggttgc caaagagtgg ccgctgtttt
    38701 cgtggtaatg gatgatgaaa aaaataattc ccgccgtagt ctgtttgttg ctttgtagtt
    38761 gcacgacctt tccgcaaact gtcgctccgg taaacggtga tgcctccaat tagttgaatt
    38821 aaggatataa tactcgcttt taaggttgtt tcatggtcac agttaacctt cattgccccc
    38881 gttgccagtc cgttcaggtt taccgtcacg ggcagaaccc aaaaagacat gatcgattcc
    38941 gcggccgtga ctgtcaccgg gtatttcagc tcagttactg ttacgagccc cgtaaaccgg
    39001 gagttaaaga ccaaatcgct gaaatgactt tcaacggtgc cggggttcgc gatactgccc
    39061 gtacgctgaa aatcggcatt aatacagtca tccgaacttt aaaaaactct cgccacgaag
    39121 aataacgtca tccccggtcg ctcatgctga tgtggcactc atctgcgaac ttagtcagca
    39181 atggagcttt gtcggcagta aagcgcgaca gcactggctc tggtacgtgt acaacacgaa
    39241 aactggtggt gttctggcct acacttttgg cccccggact gacgaaacct gtcgtgaatt
    39301 gctggcgctg cttacccttt taccatcggc atgttgacca ctgacgaatg gggtagttac
    39361 accagagagt taccgaagga aaagcatctg actgg
//
LOCUS       DAJVUJ010000015        38881 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.015, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000015 DAJVUJ010000000
VERSION     DAJVUJ010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38881
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.015"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..721
                     /locus_tag="OQQ48_000769"
     CDS             <1..721
                     /locus_tag="OQQ48_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HCS1951630.1"
                     /translation="PPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTDRKSVVLKT
                     KSGQYFVSPDNGTLTLVAQTLGIDSVREIDEKANRLKGSEKSYTFHGRDVYAYTGARL
                     ASGAITFEQVGPELPAKVVELSYQKAKATKGEVKGNIPILDIQYGNVWSNISDELLNQ
                     AEIKLNDTLCVTISEGSQQKYAGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNMDNF
                     AQKHQVASGADWNIDVKKCAK"
     gene            complement(766..2928)
                     /gene="uvrD"
                     /locus_tag="OQQ48_000770"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(766..2928)
                     /gene="uvrD"
                     /locus_tag="OQQ48_000770"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HCS1951631.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCLPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(3012..3728)
                     /gene="yigB"
                     /locus_tag="OQQ48_000771"
     CDS             complement(3012..3728)
                     /gene="yigB"
                     /locus_tag="OQQ48_000771"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HCS1951632.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(3728..4624)
                     /gene="xerC"
                     /locus_tag="OQQ48_000772"
     CDS             complement(3728..4624)
                     /gene="xerC"
                     /locus_tag="OQQ48_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCS1951633.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(4621..5328)
                     /locus_tag="OQQ48_000773"
     CDS             complement(4621..5328)
                     /locus_tag="OQQ48_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HCS1951634.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(5325..6149)
                     /gene="dapF"
                     /locus_tag="OQQ48_000774"
     CDS             complement(5325..6149)
                     /gene="dapF"
                     /locus_tag="OQQ48_000774"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HCS1951635.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(6194..6388)
                     /locus_tag="OQQ48_000775"
     CDS             complement(6194..6388)
                     /locus_tag="OQQ48_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004987677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCS1951636.1"
                     /translation="MKNVFKVLTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGMVHPR"
     gene            6851..7171
                     /gene="cyaY"
                     /locus_tag="OQQ48_000776"
     CDS             6851..7171
                     /gene="cyaY"
                     /locus_tag="OQQ48_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HCS1951637.1"
                     /translation="MNDSEFHRLADQLWLTIEEHLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(7211..9757)
                     /gene="cyaA"
                     /locus_tag="OQQ48_000777"
     CDS             complement(7211..9757)
                     /gene="cyaA"
                     /locus_tag="OQQ48_000777"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HCS1951638.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            10144..11085
                     /gene="hemC"
                     /locus_tag="OQQ48_000778"
     CDS             10144..11085
                     /gene="hemC"
                     /locus_tag="OQQ48_000778"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HCS1951639.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDARTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            11082..11822
                     /gene="hemD"
                     /locus_tag="OQQ48_000779"
     CDS             11082..11822
                     /gene="hemD"
                     /locus_tag="OQQ48_000779"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HCS1951640.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPDYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLRCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            11844..13055
                     /gene="hemX"
                     /locus_tag="OQQ48_000780"
     CDS             11844..13055
                     /gene="hemX"
                     /locus_tag="OQQ48_000780"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCS1951641.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPQ
                     GDTPAAAPQGE"
     gene            13058..14254
                     /gene="hemY"
                     /locus_tag="OQQ48_000781"
     CDS             13058..14254
                     /gene="hemY"
                     /locus_tag="OQQ48_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HCS1951642.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            14933..15094
                     /locus_tag="OQQ48_000782"
     CDS             14933..15094
                     /locus_tag="OQQ48_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1951643.1"
                     /translation="MEFSFSPKLLVVAVAAALPLMASAADTPSTATARKGFAGYDHPN
                     QYPQNAAIY"
     gene            complement(15178..16413)
                     /locus_tag="OQQ48_000783"
     CDS             complement(15178..16413)
                     /locus_tag="OQQ48_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCS1951644.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLDFYRRAVKLQAKYGAGRKISN
                     SFQTNGVLLDDKWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAASDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLLVVNK"
     gene            complement(16560..16636)
                     /locus_tag="OQQ48_000784"
     tRNA            complement(16560..16636)
                     /locus_tag="OQQ48_000784"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16600..16602),aa:Pro,seq:tgg)
     gene            complement(16679..16765)
                     /locus_tag="OQQ48_000785"
     tRNA            complement(16679..16765)
                     /locus_tag="OQQ48_000785"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16729..16731),aa:Leu,seq:cag)
     gene            complement(16786..16861)
                     /locus_tag="OQQ48_000786"
     tRNA            complement(16786..16861)
                     /locus_tag="OQQ48_000786"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16826..16828),aa:His,seq:gtg)
     gene            complement(16920..16996)
                     /locus_tag="OQQ48_000787"
     tRNA            complement(16920..16996)
                     /locus_tag="OQQ48_000787"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16960..16962),aa:Arg,seq:ccg)
     gene            complement(17099..18484)
                     /locus_tag="OQQ48_000788"
     CDS             complement(17099..18484)
                     /locus_tag="OQQ48_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCS1951645.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAVGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNESGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(18675..19415)
                     /gene="rffM"
                     /locus_tag="OQQ48_000789"
     CDS             complement(18675..19415)
                     /gene="rffM"
                     /locus_tag="OQQ48_000789"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HCS1951646.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(19418..20770)
                     /gene="wzyE"
                     /locus_tag="OQQ48_000790"
     CDS             complement(19418..20770)
                     /gene="wzyE"
                     /locus_tag="OQQ48_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HCS1951647.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTREVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNREPNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(20767..21846)
                     /gene="rffT"
                     /locus_tag="OQQ48_000791"
                     /gene_synonym="wecF"
     CDS             complement(20767..21846)
                     /gene="rffT"
                     /locus_tag="OQQ48_000791"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HCS1951648.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNDHIAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRVLAIAAGEVA"
     gene            complement(21843..23093)
                     /gene="wzxE"
                     /locus_tag="OQQ48_000792"
     CDS             complement(21843..23093)
                     /gene="wzxE"
                     /locus_tag="OQQ48_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HCS1951649.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(23095..24225)
                     /gene="rffA"
                     /locus_tag="OQQ48_000793"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(23095..24225)
                     /gene="rffA"
                     /locus_tag="OQQ48_000793"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HCS1951650.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(24230..24904)
                     /gene="rffC"
                     /locus_tag="OQQ48_000794"
                     /gene_synonym="wecD"
     CDS             complement(24230..24904)
                     /gene="rffC"
                     /locus_tag="OQQ48_000794"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HCS1951651.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(24882..25763)
                     /gene="rfbA"
                     /locus_tag="OQQ48_000795"
     CDS             complement(24882..25763)
                     /gene="rfbA"
                     /locus_tag="OQQ48_000795"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCS1951652.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLVQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDQERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(25782..26849)
                     /gene="rffG"
                     /locus_tag="OQQ48_000796"
     CDS             complement(25782..26849)
                     /gene="rffG"
                     /locus_tag="OQQ48_000796"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCS1951653.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLIYAGNLMSL
                     APVAQSDRFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVVHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(26849..28111)
                     /gene="wecC"
                     /locus_tag="OQQ48_000797"
     CDS             complement(26849..28111)
                     /gene="wecC"
                     /locus_tag="OQQ48_000797"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HCS1951654.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSTRASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(28108..29238)
                     /gene="wecB"
                     /locus_tag="OQQ48_000798"
     CDS             complement(28108..29238)
                     /gene="wecB"
                     /locus_tag="OQQ48_000798"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HCS1951655.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKAPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELVANYPFIDPNKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(29294..30340)
                     /gene="wzzE"
                     /locus_tag="OQQ48_000799"
     CDS             complement(29294..30340)
                     /gene="wzzE"
                     /locus_tag="OQQ48_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HCS1951656.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(30352..31455)
                     /gene="wecA"
                     /locus_tag="OQQ48_000800"
     CDS             complement(30352..31455)
                     /gene="wecA"
                     /locus_tag="OQQ48_000800"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HCS1951657.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMALGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(31695..32954)
                     /gene="rho"
                     /locus_tag="OQQ48_000801"
     CDS             complement(31695..32954)
                     /gene="rho"
                     /locus_tag="OQQ48_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCS1951658.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(33039..33140)
                     /gene="rhoL"
                     /locus_tag="OQQ48_000802"
     CDS             complement(33039..33140)
                     /gene="rhoL"
                     /locus_tag="OQQ48_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HCS1951659.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(33281..33610)
                     /gene="trxA"
                     /locus_tag="OQQ48_000803"
     CDS             complement(33281..33610)
                     /gene="trxA"
                     /locus_tag="OQQ48_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HCS1951660.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            33741..35006
                     /gene="rhlB"
                     /locus_tag="OQQ48_000804"
     CDS             33741..35006
                     /gene="rhlB"
                     /locus_tag="OQQ48_000804"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HCS1951661.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEVLEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            35140..36624
                     /gene="gppA"
                     /locus_tag="OQQ48_000805"
     CDS             35140..36624
                     /gene="gppA"
                     /locus_tag="OQQ48_000805"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HCS1951662.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVEKEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(36671..38692)
                     /gene="rep"
                     /locus_tag="OQQ48_000806"
     CDS             complement(36671..38692)
                     /gene="rep"
                     /locus_tag="OQQ48_000806"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HCS1951663.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKYYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
BASE COUNT         9615 a        10629 c         9841 g         8796 t
ORIGIN      
        1 tccaccgtat aacatctggg aaggcgctta tcgcctgtat cagactgcca gttactggcc
       61 gcaaggttcg gtgtttgtta gcgtggtcga tccgggcgta ggcactgacc gtaaatcggt
      121 agtgctgaaa accaaaagcg gtcaatactt cgtttcacca gataacggta cgctcacttt
      181 ggtggcacaa actctgggta ttgatagcgt ccgggaaatc gacgaaaaag ctaaccgcct
      241 gaaaggttct gaaaagtcat ataccttcca cggtcgtgac gtgtatgcct acaccggtgc
      301 acgtctggct tccggggcga ttacgttcga acaggtgggg ccagagcttc cagcaaaagt
      361 tgtcgaactt tcttatcaga aagcaaaagc aacaaaaggt gaagtgaagg gcaatatccc
      421 gattctcgat attcagtacg gcaacgtctg gagcaatatc agcgacgagt tgctgaatca
      481 ggcagaaatc aaactcaacg acacgttgtg tgtgaccatt tccgaaggtt cccagcaaaa
      541 atacgcaggg aaaatgccgt atgtcgcaag cttcggcgat gtgccggaag gccagccgat
      601 ggtgtatctg aacagcttat tgaatgtttc cgtggcgctg aatatggata atttcgcgca
      661 gaaacatcag gttgcgtctg gtgctgactg gaatattgat gtgaagaagt gcgctaagta
      721 aggtgctgat gcggaatgcg gtgtggcgca ccgcatcccg caacgtcaca ccgtctccaa
      781 ccgggcataa gccgccacca gccatttaat accctggccc tggaatgcca cctgcaaacg
      841 gctatgttca ccgctgcctt ccatattgac gatggtgcct tcaccaaact tagcgtggcg
      901 tacacgctgg ccgagcttgt agccgctgtc gttctcgacc atcggcgtac ccatccgctg
      961 atggctgacc gggcggctta ccgtggcgcg caggcgcacc tcttccacac actcttccgg
     1021 cagctcgcca ataaagcgcg acgggcgatg gtaaacctct ttgccataca gacggcgagt
     1081 ttccgcgtag gtcagcgtca gtttctgcat cgcgcgggtt acgccaacgt aggccagacg
     1141 gcgttcttct tccagacgcc cgccttcatc cagcgacatc tggcttggga acatgccctc
     1201 ttccataccg acgataaaca cctgcgggaa ctccagacct ttcgccgagt gcatggtcat
     1261 cagttgtacc gcatcctgcc aggtatccgc ctgcccttcg cctgcttcca gcgccgcatg
     1321 ggataggaac gcctgcagcg gcattaaatc ttcgtcttct tcgttgtagc tgaactggcg
     1381 cgttgccgtc accagttcct ctaagttttc gatacgcgtc tgaccttttt cgcccttctc
     1441 ctgctcgtac atggtacgca ggccggagtc tttaattacc cggtcagtct gtacatgcag
     1501 cggcatatcg gcagtttcct gcgctaaggc gtcgatcagt tccataaacc gctgtaaggc
     1561 gctggcagca cgtccggcga gggctttttc ctgcaacagt tcacgacatg cctgccagag
     1621 tgttaactgg cgatcgcgcg atgtctgacg taccacgtcc agcgtccggt caccaatacc
     1681 ccgcgttggc gtattcacca cacgctcaaa ggccgcgtcg tcgttacggt tggcaatcag
     1741 gcgcagatac gagagcgcat ctttgatttc ctggcgttcg aagaagcgca tcccgccgta
     1801 aatacggtac ggcatactcg cctgcaacag tgcttcttcc agcacacgcg actgggcgtt
     1861 actgcggtag agaatggcgc actcggcaag cgccccgccg ttgtcctgcc aggttttgat
     1921 gcggttaacc acaaaacgcg cttcatcgag ttcgttaaaa gcgcaataga gggaaatagg
     1981 ctcaccgtcc gcgccatcgg tccacagttt tttacccaga cgcccgttat tgttttcaat
     2041 cagggcgtta gcggcgctca gaatattgct ggtagagcgg tagttttgct ccagacgaat
     2101 agtttcggca ccggggaaat cattaaggaa acgctgaata ttctccacct gcgccccgcg
     2161 ccagccgtag attgactggt cgtcatcacc gacgatcatc actttgccgg tgtcgcccgc
     2221 cagcaggcgg atccacgcgt actgaatgtt gttggtatcc tggaattcgt ccaccaggat
     2281 attggtaaaa cgttcgcggt agtgttgcag gatatgcggc ttgttaagcc acaactcgtg
     2341 agcgcgcagc agcagctcgg cgaagtccac caggcccgcg cggtcacacg cttcctgata
     2401 cgcctgatac accttctgcc aggtctgctc caccggatta ccgtagcttt gaatatgatg
     2461 tggacgcagg ccttcatctt tctggctgtt gatgtaccac attgcctggc gcggcggcca
     2521 ctgcttctcg tcgaggttca tcgccttgat taggcgtttg agcaggcgta gctggtcctc
     2581 gctgtcgagg atctgaaaat cctgcggcag attggcgtcc atatggtgcg cacgcagcag
     2641 acggtgcgcc agcccgtgga aggtgccgac ccacatgccg ccctggctgg tgcccattag
     2701 ctgcccgata cgatgacgca tctccgccgc cgctttgttg gtaaacgtca ccgccataat
     2761 cgagtatggc aagcagtttt ccacgctcat cagccaggcg atacgatgca ccagtacgcg
     2821 cgtcttacca ctgcccgcgc ccgccagcac cagaaggttg ctgcgtggcg cggccaccgc
     2881 ttcgcgctgt ttgtcattaa ggctgtcgag caggtaagaa acgtccattg gcaccgccgc
     2941 gtaaaaatag gttgggcgga gaagcgcaac gccctccgcc aaaaagctgg gtatatatac
     3001 agatttgctg attatatcag cgaggtcaga gatgccaacc gggaaatttc cagatgcggc
     3061 agtaaacggc tgtcccaggt ttgcatcaga tcgccatttt ccggtctgat ccaacaggcc
     3121 tgcattccgc tgcgaattgc cccacccacg tcagtggtga gatcgtcccc aacatgtaag
     3181 atctcgccga tcggcacgtt gagtttttcc gcagccagaa agtacatatc gctgaacggt
     3241 tttgagcgcc cgtgcgggcc agcgcgcagc acaaactcaa aataatcccc caaaccaaac
     3301 agttccggct gggcgttacc gttggtgatc gccaccagcg gccatttctt cgccagctgt
     3361 tttaaggtgt cgtgagtttg ctgcgggacg tcgattcggc tgcgccattt ggcaaagttg
     3421 atcattgctg cgtgtgcgcc tgcactggct tcttcggcac tcagcccggc gtcgagcatc
     3481 gcttgttcaa tcgaacgaaa acgccagcgc gtcacgtcgt gataaatctc gggttccgct
     3541 tcccgtaccg cctggcgcag gcgttgcaga tcttcattct ggaagctgcg cagcgccgga
     3601 tgataatttt gcacaaaggt aagcgcctct cgctcggtgc gcaaaatcac cggacggtta
     3661 tcgtaaaggg tatcatccag gtcaaaggtg agcgccgaga tgcgccccaa aggccggtaa
     3721 aaacgcatta tttcccccgt ttggcgcgtg gatgcgccgc atcgtacacc gaggcaaggt
     3781 gttgaaaatc aagatgagta tagatttgcg tggtggagag gttggcatga cccagtagct
     3841 cctgcacacc acgaagatcg ccgctcgact ccagcatatg cgtagcgaac gagtgacgta
     3901 atttatgtgg gtgaacgtga ttattcagcc cttgttttat gccccattcg gcaaagcgtt
     3961 tctgcacatt acgcgcggag atacgcttgc ccagtttcga cagaaaaagc gcgtcgtctt
     4021 cgctaccaaa caggtcgcgc aaatcaagcc agtgctcaat ccacgccaca gcgttgcgac
     4081 caatcggcag gcggcgctct ttgctgcctt tccccattac ccacacttca ccagactcca
     4141 ggtcgaggtg tttgatatcc agccccacca gctcggaaag acgcagacca gcgccgtaca
     4201 tcacttccag cattgcacgg tcgcgtacag cgaggggatc gttgatatca atatccagca
     4261 gccgattcat atcgtcgacg tcgatatttt tcggcagatg acgcggcgct ttcggtgccg
     4321 aaacaccttt cgccgggtta gctttgagtt cgttctggct gaccagccag tcaaaaaagc
     4381 tacgtagcgc agaaagccgt aacgccagac ttgctgctcc cagcccttta cggcgactgc
     4441 gtacagcaaa attgcgcacc atcgtcacat cacattgctg ccagctttgc aggccgtttt
     4501 cgctggcaaa attgatgatc gcctcaagct gacgctggta gttaagcagg gttatcgggc
     4561 taagctggcg ctccacgctc agataacgta ggtagcgttc tacatcggtg tgtaaatcgg
     4621 tcatacgcgt tcaatccaac gctccagaag ctccggcaac atcagcgcaa tttcatgaag
     4681 taactgcgtt ccttgccctt gttgatagtg actggcatcg cgactggtaa acagcacgac
     4741 acccaaatca gcatcgcttc ccagcatcga catcgccacc gatcccaccg ctttcgcttc
     4801 cggtagcacc accagcagct ctggtccgtt aagcggccca agatagtgct gttcctgccc
     4861 caaacgctga atacgcagcg gttcgaaaga ctgacggctt aatgccagat gagtgtggtt
     4921 cgacggcgca cctaagcgcc agcgatccgg aaacaggcgc agactcgcac ctgccaggcc
     4981 gagatcgcgc gcccagcggt gaaagcgcat cagcatatcg tcgagactgc tggcggcggt
     5041 gagactgcgc tgcaggtaga gtaggcgata aaacaggcct tcgttggcga tagcctgttc
     5101 catcaacagc gccatgttct cttccagaac atgaatatga ttacgtgcgc gggccatgtg
     5161 ccactcgacc aacgaaacgg tgccgcgtac cggatgtggc acacgtatcg cttctactgc
     5221 gcgcgcatta cggataaaaa actcaggatt tttaatcaga taatcgacaa ccgcccggtc
     5281 atcaagctcc gtgagtgttt cctgcagttc ttcccctggt tgcttcatag atgaataaat
     5341 ccgtcgtaga catgtaccgc cgggccagtc atatataacg ggtgacccgg acctttccag
     5401 gcgatatcaa gacgaccgcc ggggagttcc acgcgtactt cttcggccag caaaccttgc
     5461 tgaatcccta ctgcaaccgc cgcacacgcg ccgctgccgc aggcctgggt ttctcctgcc
     5521 ccacgctcat aaacgcgtaa acgaatatgc tcgcgcttaa ccacttgcat aaaaccgata
     5581 ttggcgcgct ccggaaaacg ctcgtggctt tccagaacag gaccaagcgt ttctaccgcc
     5641 gcggtatcga catcatcgac ctgaatcacg caatgcggat ttcccatcga caccacgccg
     5701 cataagattg tctgctcggc ggcgcgcata atataggtct tttccgcttt gttagcgcga
     5761 aacggcacag cggaaggttc gaagttgggt tcgcccatat ttacgcggac cagatcatca
     5821 tcggtgacgg tcagaaccat ccgcccgttg gcggtgctga cgcggatatc gcgcttattg
     5881 gtcagccctt tcaggcgcac aaaacgggca aagcagcgcg caccgttgcc gcactgcgcc
     5941 acttcactgc catcagcatt gaaaatgcga tagtgaaaat ccagttcagg atcatacggc
     6001 ggctcaacca ccagcagctg gtcaaaccct acccccaggt gccgatccgc cagccgacga
     6061 atcagctccg gtgaaaaaaa gacattctgc gttaccgcgt cgacgaccat aaaatcgttg
     6121 ccaaggccat gcattttcga gaactgcatc atttactcca atcacgcggg tacagaaact
     6181 gacttttagt aattcacctg ggatggacca tcccagtggc gcgatcgttt ttatccggca
     6241 ccgtggattg cgtttgcgtc tctaccggtt tggtcggcgg cggtgcattt ttatctgcag
     6301 gcgggaaata gagcggacct ttcagaccgc agcccgtcag gctgaagaga gtaagtaata
     6361 cagtgagtac cttaaacacg tttttcatta tggattgcct gtaaagttca tcgctttctg
     6421 ctttctatca tcgcaggaga aggcgtaaaa gcaagcgttt agcgactctt tatgaagtct
     6481 gaaaagtgag gaaatgctgc gagtataacg cttactcgtg aggatttatg ttctgctggc
     6541 agtttcgtga tcggcacctc gcgccaggat ggctttatgc actatcgcaa tgtggtttgt
     6601 ttgttggctt ttttagttcc gtcagcgtga ggatgctcgc tcggtaaacc tgatcataat
     6661 gtttaccaga aacagggaaa ggcgtggtgt aactgcgccc taatgaagag acaaatcttt
     6721 cgccacacag agacatcatt attccggtga cggatgaatc atgccggaac gcagcagcag
     6781 aggtgcaaac tttgaagccc tgaccccatg ccgctatact gccgccatca cttaaaaaca
     6841 ggatacaaca atgaacgaca gtgaatttca tcgcctggct gatcaactgt ggctgaccat
     6901 tgaagagcat ctggacgact gggatggcga cagcgatatc gactgcgaaa tcaacggcgg
     6961 cgtgctgacc attacctttg aaaacggcag caaaatcatt atcaaccgcc aggaaccgct
     7021 gcaccaggta tggctggcaa ccaaacaagg cggctaccat tttgatctga aaggcgatga
     7081 gtggatttgc gatcgcagcg gcgaaacctt ctgggatttg ctggaacagg cggcgacgca
     7141 gcaggcgggt gaaacagtca gtttccgcta agattgcatg ccggataagc ctcgctttcc
     7201 ggcacgttca tcacgaaaaa tattgctgta atagcggcgt atcgtgatcc tgattggcag
     7261 gcggcatgtt accgatagat tttgtgcgga acggaatcac ctgttcacga ccatcaacct
     7321 tcacaatctg atagaactgc ggcaggttga agttgatgaa gcttgagccg taggtgaagc
     7381 ggtcgtgcga tgacgagtag aagcgactga cgtcacgcac cagttcctct ttgctgcctt
     7441 cgcagtggtg atacacctca acccggttgc tttcgtcaag aatgtagata ttaaagccat
     7501 tctcgtcttg cgtttcttcg aagaagaact ggatgatccc ttcactagca aagccgtcca
     7561 ccaccgccgg taatttgacg tgattggttt caacctgcac tgacaggccg tgcagtttgt
     7621 tatgcgaaat agcgccataa aactcgatgg cgttctccag cttctgtacc gatacattca
     7681 ggcgttcgaa gaacaagccc caggtttgac cagaaacgcg cagcgccttg aaacgcccgg
     7741 tttcctggcg ggtgctggaa aggcgcagtt caatacactc agaaaccagt tgctgcacgc
     7801 gagtacggat taacccgcgc agatgctggc tataacagaa gacttccacg ctatctggcg
     7861 gtgcggcgtc ctgatgcatt ttgccgagaa tagttttcag ggcttcgatc atcgattgct
     7921 cgccgttgaa gtgcagcgta cgcacttcgt tccacgagtt gcggtacagc aggtcaacgc
     7981 tacctaccag gcaattttga ttctcgccaa agctgaagac atccagctta cggaaatcaa
     8041 aatgcaccac ctgattgcgg aacgccgctg tcgggtcata ttccaggtta acgataatcg
     8101 ccagatggcg gatctcacac gggctgtaga gcgccttcgg tgtcggtgca ggtaagcgca
     8161 gcgggaaatg gtgcgacacg tcggcgacca tctcctgcaa cttaggcaaa tcgacaatgc
     8221 cgttaccttt aatatacaaa cgggtgcgcg aggtcagcag gccgttaaac catgcccacg
     8281 ccaccagttt gttcaggtaa cggttatatt ccagcggctg atggctgatg atcgactcaa
     8341 tatttggcgc gcggttatac agataccaac ctgaacggtt agcacggccc ggcggcacat
     8401 aaataaaggt cagattcggt tccgagagat cgggtgaaat ctgcgggttt accagcgtca
     8461 ctttacctgg taatgcttca aacgcggcat acagcttacg cgtcagcacg ccgatatcct
     8521 gcggactggc ggagacgcta aggttattgc gacgcgcaaa gcggatcaga ttacggtagc
     8581 tctgcatcat cgcgtcgagc aactcgttgt gcgcctcacg cacctgatca atcttccagt
     8641 tagcgcggtt atcgagcatt gccagacgag cttcgtccca accccactcg ctcactaact
     8701 ggctcaacac tgcgcgacgc cagcctacgc aggcgcgttc acggctgagc ttttcgcaca
     8761 cttttaaata gaagcagcga cgtactaaat ccagacgggt aaaatcttca atcgccgtca
     8821 ggtattcagt aacacgctcc agcatcatgc agtatggatc gagaccaaac gatacaatct
     8881 caccgtcgtg caaacgctgt ttgatatctt tcgccagcag acgtgggttc gggtattccc
     8941 aggaataggc ttccagcagc agtgttttca gtaccgcttt gtatggggaa tcgatactct
     9001 tgtagagctg ccaaaggctg gcaccaaagt actcttcagc agaaagcgag cttaagccac
     9061 cgagatccag ccattcattt ggcgtcagca cgccctgcgc gtaaagcgtc atcacatagt
     9121 cgtcgtaatg ctcttcttcg tcgcacggca ccatattcca cagaatacgc ttaccggcga
     9181 gacgcacggc ggtacgataa aattcgtcaa gcagcagtat atgctgggtg gagccacaat
     9241 cttcgccccc caagctgccg ctttcattat gacggaagcg gttttcatca atcaggaaga
     9301 agctgacttc cacacccagc gaggcggccc agctttccag cagactacat ttacgttgta
     9361 gtaactggcg ctcttcgcta tcgagccagg attgatgaca gacccagata tccaggtcag
     9421 aggaacaact ttgccctacg gacgaggtgc tgcccatggt gtatacacca gtaattggaa
     9481 gctcaccttt cggtggatcc tgtactgaca ttccacgata cagttcaagc tcgttcaggt
     9541 agtggcgttg agtttcatca ggcgtgtaaa ggcaaatgcc tttgggaacg ttaccatcaa
     9601 ggtaacccgg cattagcgga tggtgatagt gcaacaatgt cggcagtaga ctgtagacct
     9661 gttggaatgc aggccccata gcagcaagcg cgcgatccac acgcaattga tttatggcat
     9721 ccagtctctg tttcagagtc tcaatataga ggtacaagac gtatcgcctg atttgctacc
     9781 cgtcatgact gtgattccgc caacatcaac ggtaacacgc ggcattcggg atatttcgta
     9841 tgtcaaaggt aaccgttacc acttttcgcg cctggttttt ttagtttcac gacgaaaaaa
     9901 tggtctaaaa cgtgatcaat ttaacacctt gctgattgac cgtaaagaaa gatgcgctac
     9961 atacaagtgt agcaccgttt attctctgta aattccttat tacgacggcg tgaaacgcct
    10021 gtcaggatcc actgccagac ctcattttac ggtttgcgca ggcgtctacg tttcaccaca
    10081 acactgacat cactctggca aggatgttag gatggaccac ggatgataat gacggtaaca
    10141 agcatgttag acaatgtttt aagaattgcc acacgccaaa gcccacttgc actctggcag
    10201 gcacactatg tcaaagacaa gttgatggcg agccatccgg gcctggtcgt tgaactggta
    10261 ccgatggtga cgcgcggcga tgtgattctt gatacgccgc tggcgaaagt aggcggaaaa
    10321 ggcttatttg taaaagagct ggaagtcgcg ctcctcgaaa atcgcgccga tatcgccgta
    10381 cattcaatga aagatgtgcc ggttgaattc ccgcaaggtc tgggactggt cactatttgt
    10441 gagcgtgaag atcctcgcga tgcctttgtg tccaataact atgacagtct ggatgcgtta
    10501 ccagcaggca gtatcgtcgg gacgtccagt ttacgtcgcc agtgccaact ggctgaacgc
    10561 cgcccggatc tgattatccg ctccctgcgc ggcaacgtcg gcactcgcct gagcaaacta
    10621 gataacggcg aatacgatgc catcattctt gccgtagccg gactaaaacg tttaggtctg
    10681 gagtcccgca ttcgcgccgc gttaccaccc gagatttctc ttccagcggt aggacaaggt
    10741 gcggtcggta ttgaatgccg ccttgatgat gcacgcactc gcgagctgct tgccgcgctg
    10801 aatcaccacg aaactgcact gcgcgttacc gcagaacgcg ccatgaatac ccgtctcgaa
    10861 ggcggatgtc aggtgccaat tggtagctac gccgagctta ttgatggtga aatctggctg
    10921 cgtgcgctgg tcggcgcgcc ggacggttcg cagattattc gcggtgaacg ccgcggtgcg
    10981 ccgcaagatg ccgaacaaat ggggatttcg ctggcagaag agctactgaa taacggcgcg
    11041 cgcgagatcc tcgctgaagt ctataacgga gacgccccgg catgagtatc ctggtcaccc
    11101 gcccgtctcc cgctggagaa gagttagtga gccgtctgcg cacactgggg caggtggcct
    11161 ggcattttcc gctgattgag ttttctccgg gtcgacaatt accgcaactt gctgatcaac
    11221 tggcggcgct gggggagagc gatctgttgt ttgccctctc gcaacacgcg gttgcttttg
    11281 cccaatcaca gctgcatcag caagatcgta aatggccccg actacctgat tatttcgcca
    11341 ttggacgcac caccgcactg gcgctacata ccgtaagcgg acagaagatt ctctacccgc
    11401 aggatcggga aatcagcgaa gtcttgctac aattacctga attacaaaat attgcgggca
    11461 aacgtgcgct gatattacgt ggcaatggcg gtcgtgagct aattggggat accctgacag
    11521 cgcgcggtgc tgaggtcact ttttgtgaat gttatcaacg atgcgcaatc cattacgatg
    11581 gtgcagaaga agcgatgcgc tggcaatccc gcgaggtgac gatggtcgtt gttaccagcg
    11641 gtgaaatgtt gcagcaactc tggtcgctga tcccacaatg gtatcgtgag cactggttac
    11701 tacgctgtcg actattggtc gtcagtgagc gtttggcgaa actcgcccgg gaactgggct
    11761 ggcaagacat taaggtcgcc gataacgctg acaacgatgc gcttttacgg gcattacaat
    11821 aactctcata acaggaagcc ataatgacgg aacaagaaaa aacctccgcc gtggttgaag
    11881 agaccaggga ggccgtggac accacgtcac aacctgtcgc aacagaaaaa aagagtaaga
    11941 acaataccgc attgattctc agcgcggtgg ctatcgctat tgctctggcg gcgggcgtcg
    12001 gtttgtatgg ctggggtaaa caacaggccg tcaatcagac cgccaccagc gatgccctgg
    12061 ctaaccaact gacggcattg caaaaagccc aggagagcca aaaagccgag ctggaaggca
    12121 ttattaagca acaagctgca cagcttaagc aggcgaatcg tcagcaagaa acgctggcaa
    12181 aacagttgga tgaagtccaa caaaaggtcg ccaccatttc cggcagcgat gctaaaacct
    12241 ggctgctggc tcaggccgat tttctggtga aactcgccgg acggaagctg tggagcgatc
    12301 aggacgtcac gaccgctgca gcgttgctga aaagtgcaga tgccagcctg gcggatatga
    12361 atgacccgag tctgattacc gttcgtcggg caattaccga tgatatcgcc agcctttctg
    12421 cagtttcgca ggtggattat gacggcatca tccttaagct taatcagctt tcaaatcagg
    12481 tagataacct gcgtctggcc gataatgaca gcgatggttc gccgatggat tccgatggtg
    12541 aagagctttc cagttccatc agcgaatggc gtatcaatct gcaaaaaagc tggcagaact
    12601 ttatggacaa cttcattacg attcgccgtc gtgatgacac cgccgtaccg ctgttagcgc
    12661 caaatcagga tatctatctg cgcgaaaata ttcgctctcg cctgctggtc gcagcacaag
    12721 ctgtaccgcg tcaccaggaa gagacttatc gccaggcgct ggagaacgtc tccacctggg
    12781 tacgtgctta ctacgatact gatgatgcca ccaccaaagc gttcctcgac gaggtggacc
    12841 agttaagcca gcaaaatatc tcgatggatc ttccggaaac cctgcaaagc caggcgatgc
    12901 tggaaaaact gatgcagact cgcgtgcgta acctgctggc acaaccggca gcagggacaa
    12961 cggaagctaa acctgcacct gcacctgcac ctgcacctgc acctgcacct gcaccgcaag
    13021 gtgatactcc ggcagccgcg ccgcaaggag aataaccatg ctaaaagtgt tattactctt
    13081 tgttttgctg attgcgggga tagtggttgg cccgatgatt gccggtcatc agggttatgt
    13141 gctgatccag accgacaact acaatatcga aaccagcgtc acgggcctgg cgatcatatt
    13201 gattctggcg atggtagtgc tgtttgccat tgagtggcta ctgcggcgga tcttccgcac
    13261 tggcgcgcac acccgtgggt ggtttgtcgg gcgtaagcgt cgccgtgcac gtaagcagac
    13321 cgaacaggcg ctgctgaaac tggcggaagg cgattatcag caagttgaaa agctgatggc
    13381 gaaaaatgcc gatcacgctg aacaaccggt ggtgaactat ctactggctg ccgaagccgc
    13441 gcaacaacgt ggtgatgaag cgcgcgccaa ccaacatctg gaacgcgcag cggagctggc
    13501 cggcaatgac accattccgg tagaaatcac ccgtgtacgt ctgcaactgg cccgtaatga
    13561 aaaccatgct gcacgccacg gcgtggataa gctgctggaa gttacgccac gccatccgga
    13621 agtattacgt ctggcggaac aggcgtatat ccgcacaggt gcatggagtt ctctgctgga
    13681 tatcatccca tcaatggcga aagcccatgt tggtgatgaa gaacatcgtg caatgctgga
    13741 acaacaggca tggattggcc tgatggatca ggcgcgtgcc gataacggta gtgaaggttt
    13801 gcgtaactgg tggaaaaacc aaagccggaa aacgcgtcat caagtagcgt tgcaggtggc
    13861 aatggcggaa catcttattg aatgtgacga tcatgatact gcccagcaaa ttatcatcga
    13921 tggcctgaaa cgccagtacg acgatcgcct actgctgccg attcctcgac tgaaaaccaa
    13981 caatccggaa cagctggaaa aagtgctgcg ccagcagatc aaaaacgtcg gcgatcgccc
    14041 gctgttgtgg agcacactgg gccagtcact gatgaagcac ggagaatggc aggaagcatc
    14101 gctcgccttc cgcgcagcgc tgaaacaacg tccggacgcc tacgattacg catggcttgc
    14161 cgacgcgctg gacagactgc acaagccgga agaagctgca gcgatgcgtc gcgacggttt
    14221 gatgttaacg ttgcagaaca acccaccaca gtagttcctt ctcacccgga ggcaagcacc
    14281 tccgggacct ttctgatgca taaaaaaacg cctgctctta ttacggagca ggcgttaaaa
    14341 caggtctgta tgacaacaag tgggtgcttc actcaacgtt gtgtccatgg tgtctgatga
    14401 ggcataagcg acatctgtca gtggacgata agcaccgtaa acggtgctgc gtcattccgg
    14461 agtttatgag gcactaaggc gaacataaga gatggaatga gcatctactc gtttattatg
    14521 ccacagagaa tcgggaaata acatccctta acacttgtta tgagataatt ctgtaatcct
    14581 ctttgcttcc tgagtaataa cttcctgagt gaatatttaa cctgagcttg atcctacaca
    14641 tacttattat gaatgataaa attcattcaa ttaataacac atatattaat tgccgttaaa
    14701 actaaaaaaa gcatcaataa tcaacgcgat ataataaacc tgccttacat atcaactgcg
    14761 ccagaggtag gattgaaaac gctctcctga ttttccaatt cattttctgg ataaataaat
    14821 aatttatttt tgtcactatt atttatgtaa tcatcctgtc agggagaggg atctcaatta
    14881 tcaatgctta attacgtcat cattttgatg acatgaaacc ggagcatttt gcatggaatt
    14941 ttcgttttca cccaaacttc ttgttgttgc tgtcgccgcc gctcttcctc tcatggccag
    15001 cgcagcagat accccgtcaa ctgccaccgc acgcaaaggc tttgccggat acgatcaccc
    15061 aaaccagtat ccacagaacg ctgcaattta ttgaatttgc acgatcatgt aggccggata
    15121 aggcgtttac gccgcatccg gcaatcaacc gcaggcggcc acaaaatggc cgctatttta
    15181 cttattcacc accagcaaat gtgcctgcat aatgtcactg gccgggcgac cgtgcgccag
    15241 caaatcagcc attgctttaa gatatggcgg tagatggcgg aaataacgct gatacccggc
    15301 acacaaataa ttcagccccg gtttgccgct ggcatcgagc atgaagcggt gtttcgggca
    15361 gcctccccag cacgctttta acacgttaca actgcgacac tgcgccggta attgcttaaa
    15421 tttatcttca ccaaacacct gctgttgcgg ggaatcgatc atttctgcaa ttgtttgctg
    15481 atgcatattc cccagccgat attgcggata aacatagtga tcgcaggcgt aaacgtcgcc
    15541 gttgtgctca acaatcaccg agcgcccaca ggttggctga tgatggcaaa ccgcacccgg
    15601 cgcaccgaca aaattggcaa acgcccattc gatattcatc acgaaaatct tgccgacgtc
    15661 gcgtttgatc cagtggtcaa aaatcgccac cagaaattca ccaaattcat cggggcgcac
    15721 agaccattcc gtcagttccc cctgaatatc accaggcgca tgtagtttca gtccatcgct
    15781 ggcagctgtt tcatcagcca ggcgctcgac caccggaata aactggatga attcgactcc
    15841 cgcatcgcac aaaaaatcat aaacctgcaa cggttgctgc gcgctggtgc ggttgacgca
    15901 gaccagcacg ttatagtcga catgatgttt ttgcagcagc gtcagggcac gcatcaccag
    15961 cttatgcgtt ggtctgccac ctttggtcac gcgatattga ttgtggatct cagccggacc
    16021 gtccagcgat aacccaacaa gaaaatgatt ttctgccaga aatgcacacc atttatcatc
    16081 gagcagcacg ccgttagtct ggaagctgtt acttatcttc ctgccagcac cgtatttcgc
    16141 ctgtaacttt acggcacggc ggtaaaaatc cagcccgagt aacgttggtt cgccgccctg
    16201 ccaggtaaaa gccacttcgt tttgcggttc gctggcagcg atatagtgac ggacatacgc
    16261 ttccagcgtg tcatcgtcca tatgcgtgac tggcttttcg cggtaaaggg attgtttttc
    16321 gagataaaaa cagtagtcac agttcagatt acaatcggaa ccactcggtt tcgccatcac
    16381 atgaaaagca cgcgttggaa cctgttgcag catggtcact ccttgcctga agtcatcctt
    16441 caatacgtta agggcgaaat cataccgtgg ggcaatgagt tattacgggc gtttattgcg
    16501 aagagagggt gacgaaatgc acagaaaaca aaaaaccccg ccgaagcggg gttcaaaatt
    16561 ggtcggcgag agaggattcg aacctccgac ccactggtcc caaaccagtt gcgctaccaa
    16621 gctgcgctac tcgccgaaat actgcttttt gaatttttag ttcaattatt taaagtcgtg
    16681 gtgcgagggg ggggacttga acccccacgt ccgtaaggac actaacacct gaagctagcg
    16741 cgtctaccaa ttccgccacc ttcgcattgt cacaacttct aataatgggg tggctaatgg
    16801 gattcgaacc cacgacaact ggaatcacaa tccagggctc taccaactga gctatagcca
    16861 ccactacaaa tcttgttacg cggtattact accaccgcag ctcaagcgcc gggactaaat
    16921 ggcgcgcccg acaggattcg aacctgagac ctctgcctcc ggagggcagc gctctatcca
    16981 gctgagctac gggcgcttag cgccgttgcg ggggtggata atacggactt cccacccctc
    17041 tgtctagtcc ctttttaaat aaattgcgcg tttggttacg gtttgtgcgt tttgctgctt
    17101 attcctccag tttatgcgct ttcccgtggc gattaaggcc aaaaacttta taaatcgccg
    17161 tcaccgccag cataaagatg atgccaacaa acagcgacat acgcgtatct tcattaaagt
    17221 acatgccgat caaaacgcaa atcaggaatg ccattgttac gtagttggcc cacgggaaca
    17281 ggattgagcg gaacggatgg ctggcaatcg ccgctttatg tgcacgccga aaacgcagct
    17341 ggcttatcaa tatcacaaac catggcacca tccccggaag cacgctggca ctgtagacgt
    17401 agacaaacac acgctgcgga ttgggaatga tgtagttcag gcatgagcca attagcagaa
    17461 tagcaataga tactgccaca cccgcaactg gtacgccgtg acgggaaact ttcgccatcg
    17521 ccgccggtaa ctgacggttt ttcgccagtg cgtagagcat acgtccgcaa ctgtacatgc
    17581 cgctgttaca gccagagagc gcagccgtca gcaccacaaa gttgataatg cccgccgctg
    17641 cggtaatacc gattttggca aaagtcagta cgaacgggct gccgttgctg ccactttcat
    17701 tccacgggaa gatggtgacg ataacgaaaa tcgcgcctac gtagaaaatc aggatccgcc
    17761 acagcacttt gcctacggca ctgcgcaacg tcacctgcgg attcttcgct tcaccggcag
    17821 taatgccaat cagctccacg ccctggtagg acgccaccac aatacacagc gcggtcagga
    17881 accctttcca gccacccgca aagaaaccgc catgctctgt gagattgcta aaaccaatcg
    17941 actgcccgcc attgccaaag ccaaagaaaa tcacgcccag gccaatgaca atcatcacga
    18001 taatcgtggt gactttgatc atcgcgaacc agaactcgat ttcgccgtac aaccgcaccg
    18061 ccgccagatt cgccaacgcc accagcgcca ctgcgatcaa tgcgggtatc cactgcgcca
    18121 tctccgggaa ccagaactgg acataaacgc caacggcggt gatttctgag atccccaccg
    18181 ccatccacat aaaccaataa gaccaggcgg tgagataacc aaagaacggg ctcatataac
    18241 gatgcgcata aacggcgaac gaaccggtaa ccggttcgag gaacaacatt tcgcccattg
    18301 aacgcatgat gaaaaagacg aatagcccgg cgatgatata ggccaacaat acggatggcc
    18361 cggcccattt caaggtactg gcggccccca taaacaggcc gacgccaatg gtgcccccca
    18421 gggcgatgag ttcgatatgt cgagcttcca gcccacgctg tagctctggt ttgttatctg
    18481 ccataaatcc tcgtgttgtg tttgcatgct ttccggtgtt accggttatc gttatgggta
    18541 catcgagtgt tgcgaatgtt ttcgtaattc aggagaaatg gcaaataaag cattaaaaat
    18601 ttgaatgctt tgtgtaataa aaaagcagac aggcgacgga gtgaccactc cgtcgcttta
    18661 caaagagagg aaaatcagag gttgccggtg tagtgccagc gtaaataacg cagcaaacga
    18721 agctgacgct taatgcggct cggctgcgaa agcaggcggt agagccactc cagtcccagc
    18781 gtttgccaga ttttcggtgc gcgttttacg tgaccggtga aaacatcgta agtcccgcca
    18841 acgcccatat acagcgcatc tggatgcacc agacggcagt cgcgcatgaa gatctcctgc
    18901 tttggcgatc ccatcgcaac ggtgacgatt tgcgctccgc tggcatgaat gcgttcaaac
    18961 agcgcctgac gctgctcggg tttaaaataa ccatcctgac tgccaacgat attcacattc
    19021 cactggttgc gcagtttagc ttcagtttgc gccagcactt caggtttacc gcccacaaga
    19081 aataccggcg tcccttcttt gcctgcgcgc gccatcagct cttcccagag atcggcaccg
    19141 gcaacgcggg aaacctgcgc ctgcgggtac tttttacgta ctgaacgtac aacgctgatg
    19201 ccatccgcat atttaaattc ggcagcgtta attaactccc tgacctcggc gttatcttca
    19261 atggtcagca ttttttcagc attaatggca accagcgttc cctgcttaag ctgcccgtca
    19321 gcaaacagat aatcgagggc gtgctgcata tcacgccaac caatcaactg taagccacgc
    19381 agcgtatagg tcggtgccgt ggtgttgtta ttcattgtta tccttcaacc tgcgtccgga
    19441 gcgatgattt tgtacgttta tgaatgagtc cggcgctttc aaaaagccag tacaacagtt
    19501 ttgcgatcat cagacatgcg ccgaagacca cgataaaaaa gactacgcgt gagacaaacg
    19561 aatccagccc ttcacgtgcc agcacgatca tattgaagat cgccccaaag cagaaactgt
    19621 gcaatatcgc agccttatag cgattaggct cgcggttgcc cagctcatac agccagtcga
    19681 accatttgat gatcagacca accacgatcg ccccgagcgg gatgaacaac gcgccgccca
    19741 tcaccaccag tgagcctata agcgtaggcg agatcgccag tccggagtgg ttattcagca
    19801 cttccctggt aaagtagttg gctgagttca gcaccatact cgggcgaccc ggccacagcc
    19861 aggaagggat aaagacatag aaatcgcgga caattggagc caggccctgg aagtcgatgt
    19921 tgtcgtagtt ctgcaacagc aacgccagat tctcccacgg cgagaaggtg tcgcgagtga
    19981 gatagagaaa cgtatagaac gcttcatcgc cgctcacatt cattccatag cgttttagcg
    20041 ccagccagaa catgcccaca atccccagca cgcccgccgc cgccagcatc cacaacgaaa
    20101 tccagccgcg aataatgcca ataaacagga agatagcgaa tgcgatgatg atattggcgc
    20161 gagtgccgcc gacaatcata taagtcagca agccaaaggc gaccgtgctg acgaggaaaa
    20221 acagccacgc tttgctatcc tggcgcagaa agtagaccac cagcatcgcc gggatgaaaa
    20281 agtaaaagaa gcgttttaac gccacgccgg agacttcact ggagaagatc tgactgtagg
    20341 agttaagccg gaacagcaaa aagccgttgt gcatgaagaa gatgccgacg cttaccagcg
    20401 cgatgcccat caggatcacc cacgtaagat tggtctccac gcggttcatg gtaaacagcg
    20461 gacggcgcgg tacatcagca acgcgtttgc gtaggcgggt tttgtaggtg acatagtaaa
    20521 ccgcgtagaa gcagcccgca gaaagcaacg cctgcaacaa gatttctggc ggcgcgacac
    20581 caacatcaaa gcgaaatacc agcacgctgg tcagcgggaa gccgaagaaa aaggtgagca
    20641 aaaacagcaa tgaaaagaag acattgaagt taaagcgcac acggcgaaac tcaaaccaag
    20701 tcagcgtggc aataaacagc gtgcagagca gccagaccac aaacaggcca ctgaattgca
    20761 gcagactcat gcgacctccc cggcggcaat cgccaacacc cgctgccagc cttgtagata
    20821 gttagggcta aagaaggcaa tggtgttttt atccaccgac gccaactggc gctgcgcttc
    20881 acgcacaata tcctcgttga gatcgtcggt agtaaacagc accggcaaat gctgttccgt
    20941 catatcctgc cagaacggat tttcccggtt aagcacacaa ggaatgcccg cctgaatcag
    21001 taagcaaagc gtaccaatgc cctgctggcg ggcaaaaata aagtaaccaa gatcgcactg
    21061 acgaagtagc gccagatagg cgtcaaattc cagtttttcg ctcaaaattt gcagattttc
    21121 ttcgctgaat aactccagcc ccgcctgacg aacttcctca atgtacgctt cgttattagg
    21181 cggatatccc atcggcacca ccacttttac cgtatcgcca aattgctgat gaacggcgcg
    21241 caaggcagca atatggtcat tgctgcggtc accggagttc cccaccagaa tggtcatttt
    21301 cccttcacgt tgccgatcgt tcgccatcgt attgagagaa gggtccatcc gcgtcgggaa
    21361 gtacagcagt tcgccccgca cctttggatg cgttttggca aaaaagctca aatcaccgcg
    21421 ggtggcaaat acacagccga ctcgcttttg cgccaggcga cgtagcgggt aaaaaagctt
    21481 atatctcaag ccactggaaa gctcatacag gtctgccccc cagatatgcc agaaaaactg
    21541 gctgggctta atgccaccac tcagcagagc cagccacagt gtgggattga actgaccgtg
    21601 gaaaaaaaat cgctgctgac ggttagcttt tgctttcgcg atgaccgctt ccgccagcga
    21661 ttttttccca gggaaaaatt gcacagaaag cgccggacag ctatcactta agccgtcgtc
    21721 cttgccaaca accataaact cgcgcgcgtg ctcgctcgtc gcggccagcg cgtcattgaa
    21781 aaaccgcaaa acggttcggt tatggtgagg gatatccgat cccagtacgt gaatcagtac
    21841 agtcatgccc gcctacgcca gagtaaaaac acgccacaac aaagagaaaa atagacgata
    21901 taagttgcca tatatgcctg cgccgcgccc agtgcaccat gcgcagggat tagccagtgg
    21961 gcaaatacca tcaataaagt gaactggctg acttccgcca gaatataaaa ccgcagtgac
    22021 gctttggcga tcaccagata accaaagaca taagcgccca cttttagcac atcacccact
    22081 aactgccagg caaagagatc gcgcatagcg gtaaatttat tcgacaacag caaccagata
    22141 gcaaaatcac gcagcaacca gacggtaaaa ctcgccgccg ccactgccgg taagacgaat
    22201 tttagcgatt taaccacttc ccgggtgata tcgcgctttt ccgttagccg cgacaacgtg
    22261 ggcagcaaat atacgctgaa cgatgccgta atgaattgca ggtaggcatc ggaaatactg
    22321 ctcacccctt gccagatccc cacttcatcc cagctatact gcgccgccag cagtttacgc
    22381 atcatgatgt aagcaacagg caaggtcacc gacgtaatca acgccatgag cgtaaatttg
    22441 ctcaactgcc ctgccagacc gttatcccag ctgggtttca gatagcttaa cgggatgaca
    22501 ccacgtttga tcaacatgat ggcggcagga attaccacca gcgcgggaat cagcgccaga
    22561 cccagcaacg ccccttcata accgcccaaa cggtaactga cgtagtacgc gagaacgcca
    22621 atcaagctgc cgacaatcag cgataacgca ttacctgcgg catcgcgaaa gcctttcatc
    22681 agcgccagta acaggttgcc ccaggcgatc cccatttgca ccagcgccac taaacgcacc
    22741 agcccctgat agtcggtatt accaaacaat ccctggctga ttggcgcagc tgccagcaca
    22801 aaaaccagcg ccatcagcgt agagaaacca agtaccatcg ctgatgaagt gccgaccacg
    22861 cggcgcagct gttgcggatt atcatggtac tgggcaacgt atttggttac accgttaaag
    22921 atgccagccc cggcaagcac gccgagcacg gtaatcaact ggcggaaatt tgccgccagc
    22981 ccaagccccg ccggaccaaa tgacaccgcc agcaacttac cgaccagtaa cccggcacca
    23041 atcttgacca gtgtactggc cgccgtccac aaggacgctt ttgccaacga catatcagga
    23101 gaagtagttc aacaaagtcg caattaccgt acgctgattg acgggcgaca ggttgtagaa
    23161 cagcggcagg cgcagcaggc gctcgctctc tttggtggtg tagcgatctt caccgtggaa
    23221 ctcaccaaag tgttcccccg cagggcaacc gtgcagcgga atgtaatgaa acaccgccat
    23281 gatttccgct tctttcagaa agttaatcaa cgcgctccgg tcatcaatat cccgcagttt
    23341 aatgtagaac atatgcgcgt tctgcacgca gccatcagga atcgacggca gctcgatacg
    23401 cccggctttc gccagaggcg ctaacgcatc gtagtaattt tgccacagcg ccagacgttg
    23461 ctggttgata cgatccgctg cttccagttg cgcccacagg tatgcagctt gcagatcgga
    23521 catcaaatag ctggagccaa tatcgcgcca ggtatattta tcgacctgac cacggaagaa
    23581 ctggctgcgg ttagtgccct tttcacggat gatctcggct cgttcgatta acgctttatc
    23641 gttaatcagc gtcgcgccgc cttcaccacc cgccgtgtag tttttggttt catggaagct
    23701 aaagcagcca atatgaccaa tggttcccag tgcacgccct ttgtaagtgg acatcacgcc
    23761 ctgagcggca tcttccacca caaacaaatt atgctttttc gccaacgcca taatggtgtc
    23821 catttcgcag gccacacccg cgtaatggac cggcacgata acgcgcgttt tgtcggtgat
    23881 cgctgcttca atcaacgttt cgtcgatgtt catggtgtcc gggcgaacat ccacaaagac
    23941 gattttcgcg ccacgcagca caaaggcatt ggcggtggag acaaaggtgt agctcggcat
    24001 gatcacttca tcgccaggct ggatatcgag cagcagcgcc gccatctcca gcgaagcggt
    24061 gcaggacggc gtcagtaaca ctttggcgct gccaaaacgt tgctccagcc actgctggca
    24121 gcgacgggta aaaccgccat cgccacacag tttgccgcta cccattgccg actgcatata
    24181 gtcgagttcg gttcccacca ccggcggtgc gttaaatgga atcatgtgat cacctgtata
    24241 accagtacgc ggtgctttct acattcgcac cactttgtat gtatcgttta agcgcggcgg
    24301 tgttgcccat ttgggtcgcc acccgcaaag ttgttttacc gcgagcatac gcccagttta
    24361 gcgccgtttg catcagctca gcacctgcac cgcgtccagc cagcaggcca attcgcgcat
    24421 ctgtcgcatt gagttcccgt aaagagacat agccgcgaat atcgccggac gccgcacgta
    24481 gaatcagaca ttgatgatca aaggtgccgc gcacggcatt ttcaatccac tgtgcataaa
    24541 agcgaccgct ggcgtcaggc gcataccacg gcgcacgaaa acggctttgc gcaaatgcgg
    24601 cgctagctaa ctgacgtaat gcgggaatat cggtctcttg tgccacgaca gcaccgctat
    24661 cactgacatt gttcacgggt agcgccaaat caacttcacc ttctaccagg gagaatccca
    24721 gctgttgcag ggcatccagt tcacccgtat ttgatgccgc aattttggcc tgcacccgtg
    24781 accacggcgc taacgcgtct ggcgtcagga gcggtgcttc agacgtaatg cgcacgatgg
    24841 cgctgttaac accaaagaag gcgttttccc aggttagtgg ctcaatactg gcgcggacgg
    24901 gcacgaagta actccagcag atattggccg tagccagttt tcgctaatga actggcagca
    24961 cgcttcacac cctcgtcatc gagccagccg ttacgccagg cgatctcttc caggcaggca
    25021 atcttaaagc cctggcgttt ttctactgtc tgcacaaagg tactggcttc aatcaggctg
    25081 tcgtgagtgc cagtgtccag ccaggcaaat ccgcgcccga gcagttcaac ggtcaggttg
    25141 cccgcctcga ggtacatctg gttgatggag gtaatctcca gttcaccacg ctccgacggc
    25201 ttcacctgct ttgcgtactc cacgacttta ctgtcgtaga aataaagccc ggtcaccgcc
    25261 cagtttgact tcggctgttt tggcttttct tccagcgaga tagcgcggaa attgtcgtca
    25321 aactccacca cgccaaagcg ttcctggtcc atcacctgat agccaaatac cgtcgcccct
    25381 tccgtgcgcg ccgcaacatg acgcagcttc ggactgaagc cctgaccgaa gaagatgtta
    25441 tcgcccagca ccagacaaga aggttcaccg ttgaggaagg tttcaccgat gataaaggcc
    25501 tgcaccagac cgtccgggct gggctgttcg gcgtattcca gctggatacc gaactcacta
    25561 ccatcgccca gcaggcgctg gaaataacct ttatcttccg gcgtagtgat gatgagaatt
    25621 tcgcggatac cggccaacat cagcaccgac agcggatagt aaatcattgg cttatcgtaa
    25681 atcggcaaca gttgcttcga tacgccgcgc gtaatcggat gcaatcgggt gccggaaccg
    25741 cccgccagga taataccttt catgtttgcc tccgctgaaa attagccttt cagacctaaa
    25801 cgctcgccct gatagctgcc gtcctgcacc tgcttccacc agctttcatt agccagatac
    25861 cactgcaccg ttttacgcat tccactttca aaggtttcct gtggcagcca gccaagttca
    25921 cgggcaattt tcgaagcatc aatggcataa cgcagatcat gccccggacg gtcagcgaca
    25981 aaggtgatca agtcacgata gtgcaccacg ccgtgcggct tgttcggagc cagttcttcc
    26041 agcagctcgc aaatggtttc cacaacatcg agattcttac gctcgttgtg accaccaata
    26101 ttataggttt caccgacttt cccggtggtc gccacgcaat acagcgcgcg ggcgtgatct
    26161 tccacataca gccagtcacg gatttgctgc ccgttgccat ataccggcag cggtttaccc
    26221 gccagcgcgt tgaggatcat cagcgggatc agtttttccg gaaagtggta aggaccgtag
    26281 ttattcgagc agttggtgat aagcgtcggc agaccgtagg tccgcagcca ggcgcgcacc
    26341 agatggtcgc tgctggcttt tgacgcggaa taagggctgc tcggcgcata cggcgtggtt
    26401 tcggtgaaga aatcatccgt cgagtgcagg tcaccatata cttcgtcggt ggagatatga
    26461 tgaaaacgga acgctgattt tttatcttcc gtcagcgtat tccagtaagc ccgcgccgct
    26521 tcaagcaatg tataagtccc gacaatgttg gtttcaataa acgctgccgg gccgtcaata
    26581 gaacggtcaa catggctttc ggctgccaga tgcatgacac agtctggctg atgctcagtg
    26641 aatacgcgtg ccagttctgc ccgatcgcag atatcaactt tctcaaaggc aaagcgatcg
    26701 ctttgcgcga ccggtgccag cgacatcagg tttccggcgt agatcagctt atcgaccact
    26761 accaccgcgt cgctcgtttc gttgatgata taacgcacca gcgccgagcc aataaacccg
    26821 gcaccacctg ttatcagaat ttttctcatc agcgccagac tcctttggca tcgacgacat
    26881 actgctgatg gacattgtcg ccattgataa ctttaaactg actatgatcg accagcatca
    26941 ccagcacgtc tgccgttgcc agcgcctcgt caagctgcgc cagagtacaa agcccggtca
    27001 gttttttcgg caactggtgg atgttaggct caacaaccag agtttcgccg ctatgccact
    27061 gggcgatcag ttcagcgatt tccatcgccg ggctttcgcg caggtcatca atattcggtt
    27121 taaacgccag accaaagcag gcgattttca gttcactggc gcgtttatcg gtagccgcca
    27181 ggcaatcagc caccgccgct ttcacctgat cgataaccca gaacggtttg tgatcgttca
    27241 cttcgcgcgc ggtacggata agccgcgcct gctgggggtt ctgtgccacg ataaaccacg
    27301 gatcaacagc aatgcagtga ccgcccacgc cagggccagg ctgaagaata ttaacgcgag
    27361 ggtgacgatt cgccaggcga atcagttccc agacgttaat cccctgatcg gcacaaatca
    27421 gcgacaattc attagcaaaa gcgatattca catcgcggaa gctgttttcg gtgagcttac
    27481 acatttccgc cgtccgcgag ttagtgacga cacactcacc ttcgaggaaa attttgtaca
    27541 gttcgctggc gcgggtcgaa caaaccggcg tcataccacc aatcacgcga tcgtttttaa
    27601 tcagctcgac cataacctgg cccggtaaca cgcgttccgg gcagtaagca atgttgacgt
    27661 ccgcctgctc gcccacctgc tgtgggaaag tgagatccgg acgcatctct gctaaccatt
    27721 ctgccatctt ctcggttgac cccaccggcg aggtggattc aaggatcacc agcgcgcctt
    27781 ttttcagcac tggcgcaatg gagcgagcag ccgattcaac gtaggtcata tctggctcat
    27841 gatcgccttt aaacggcgtg ggtacagcaa tcagccaggc atccgcttca actggcgtcg
    27901 tgctcgctcg taaaaaaccg ccttctacgg cagtttttac tacgctcgcc aaatcaggct
    27961 cgacgatatg gatttcgcca cgattgatgg tatcaaccgc gtgttggttg atatcgacac
    28021 caatcacctg tttttgccgt gaggcaaacg ctgctgctgt aggcagcccg atgtaaccca
    28081 gtccgataac agaaatggtc gcaaaactca tagtgatatc cgattatttt ttaacgcttc
    28141 cagaatgcga gagcatgcct gaccatcacc atacgggtta tgggcgcggc tcatagtttg
    28201 atattcgttt tcgtctttta aaagacgcgt cacttcctcg acaattcgct gcttatccgt
    28261 gcctaccaga cgcaccgtac ccgccgtcac cgcttccgga cgctcagtgg tatcgcgcat
    28321 caccagcacc ggtttcccca gcgaaggcgc ttcttcctga atgccgcctg agtcggtcaa
    28381 aatcagccag gcgtggttca tcaaccagac aaacggtaaa tactcctggg gatcgatcag
    28441 aatgacattt ttcacatgcc ccagaatgcg attgaccggc tctctgacgt tcgggttgag
    28501 atgcaccgga tagacaatct ggatgtcctg gtgcgtggtg gcaatgtctg ccaacgcgtg
    28561 gcagatttct tcaaaaccac gaccgaaact ctcacgcctg tgaccggtca ccagaatcat
    28621 ctttttattg gggtcgataa acgggtaatt tgccaccagt tctgaacgca gcgtgtcgct
    28681 gctcatcacc tgatcacgca cccataacag tgcatcaatg actgtattac cagtaatgaa
    28741 gattcggcta tccgcaacgt tttcacgcag caagttttgc cgggaagttt cggttggaga
    28801 gaagtgatac atcgccagat gcccggtcaa tgtacggtta gcctcttccg gccacggcga
    28861 atagagatcg cccgtgcgta aaccagcctc aacgtgacca acaggaatac gctgataaaa
    28921 cgccgccagg ctggttgcca gcgtcgttgt tgtgtcgccg tgaaccagca cgacgtctgg
    28981 tttgaactcg gcaagaatag gttttagccc ttccagaatc cgacaggtta tctctgtcag
    29041 gccctgtcct ggctgcatta tgttgagatc gtagtcaggt acaatggaaa agagtttcag
    29101 cacctgatcg agcatctccc gatgctgcgc agtgacgcaa actttagcct caaaaaaagg
    29161 agcttttgcc aacgcatgca ccaacggagc catcttgatg gcttccgggc gcgtaccaaa
    29221 tacagtcagt actttcacat cgattctctt cgaataagcg gcgagcgcct ttgcgctcgc
    29281 cgcagcagtg ttgctatttc gagcaacggc gggttaatgc gacaccagcc ccgatcagcc
    29341 ccccgacaat gccccacata atcatcagga aggcacgacg tgggctatcg cgttttaccg
    29401 gttcttccgg cgtacgcaaa tagcgatagg tctgaaaacg cggatccagg gttggaccaa
    29461 cattcagggt gtttaacatg gcccgattct gatcatagtc gagatcaaag gccggaccga
    29521 cggcctgtaa attttccagt cgagcctgga gcattggacg cccaagcagg aacatttctg
    29581 aatcaggtaa ttcctcggca ggcacatctg tcgcactgcg cgaaatatta tgctgctcag
    29641 caattttcag cgcctgctca atgctgttca tccggcggtc gtagatggct ttcgccacct
    29701 cttcctgacg cttcacctga gctttcatct ggatggtgcg tgccgcccat gcgcctttca
    29761 gctcatcatt cagatggctg gctgcacgct ggctggcaaa agcaacatac tgacgtaaca
    29821 ggttattagc gtcaggcgcg gtttcggcaa taagcttcac gctgtcattg accgcgcggg
    29881 taaagtctcc ggggataaac tggatgttgt taatcatttc atccagcaac gccgcatcgg
    29941 ctttgctgtt gcccaccatc cgctgtttgt aatagtcggt ttgcagccag aactctctgc
    30001 gggtatccca cgaggccagt tgcataacaa actctttata ggcttcgtcc atgaccgatg
    30061 gttgatcggc agaagccatg tttgaacgga catccaggtt acgcaaaaat tgctgctgcg
    30121 agtaatatcc ccccagcata ttcaccgttg gacgatcggt aatcgccgtc gagctccact
    30181 cctgacgagc aaaaaaagta tacgccagcg cgattaacgc aaacgccagc cccatgccaa
    30241 taatccatag cttcccagcc cacaaggtac gaaacaaccc acgaatatcc agttcatttt
    30301 cagcgtcttc ggccggtttc ccaggcattg gttgtgtcat cacatcctca tttatttggt
    30361 taaattgggg ctgccaccac gatttctacg cagtctgcgt tttacgcgct taataaagcg
    30421 agcaactttc caggcacgct taatgcaata tccatagagg aagaatgcta gcaaaaagag
    30481 caccagcatg acccactccg ggacaaaatg agaatattct gccagcacgc caatggaagc
    30541 gagcagtgct gcggcaaggg taatcagcac aaacgcctga cgggaagtaa acccggcacg
    30601 catgatcaaa tggtgaatat gctgacggtc aggagagaat gggctcatgc ctttacgcag
    30661 gcgacggtac ataatcgcca ccatatccat tagcggaatg gcgattatcc acaaagcggt
    30721 aaccgggctg atgggatggg ttttgccctg ggtcgtttcg agcaggatcc agataacggt
    30781 aaaaccaatc agcgtactgc ccgcatcacc cataaagact ttgtagcggc gacccaggat
    30841 accaaggtta agcatgatgt atggcaggat ggcggcgatc atcgcaaagc accagattgc
    30901 gaggctggtt tgcccgtcga accacaaaat cataccgatt gctgcaaacg agacgcagga
    30961 caacccgccc agcaagccat caatgccatc aaccatgttg aacgcattaa tggccgccca
    31021 gacggcaaat agcgtcagga agtaaccaaa cggtccgagc gccatctccc aggagccaaa
    31081 gatataaccc aggctactga gataaagttt gccgaacacc atcataacaa tgccaacagc
    31141 ggcctgtatg gtggcacgga tttttacgct gatatcaaaa cggtcatcca gcgcgccaat
    31201 gaaaacaagc acaccggcac aagcgagata gagagatgca tgcggaatat agtaatcgac
    31261 aattccgaac gtgaagcaaa tccctgcgta aaccgaaatc cccccaacga gaggtatcaa
    31321 tccctggtga cgtttgcgga agtttggttt atccactaaa ccgacttttt ttgccacctt
    31381 acgggcaaaa aacagaaaca gtgtcgtgaa taaaaaaata ctgatgagat cagtactcac
    31441 tgtcagtaaa ttcacaatgc atgctctcag agaaaattat tagcagaagt ataaccacga
    31501 agacctttat tcagaaggga aagtccgttc cgaacccacc agtcctgtgc aaaaattaac
    31561 catttgatat gcaaattgca taacatgcca gcaaattcca ggaattttcc taacatcgcc
    31621 agcgcggcat taagattaca gatataaaag caaaacgcca cgtaaacacg tggcgttttt
    31681 ggcataagac aaatttatga gcgtttcatc atttcgaaga aatcgtcatt ggtcttggtc
    31741 atggccagtt tattaatgag gaattccatt gcatcgattt cgcccatcgg gtgaatgatt
    31801 ttgcgcagga tccacatttt ctgcagttct tcctgagtcg tgagcagctc ttctttacgg
    31861 gtaccggaac ggttgtagtc gatagccggg aagacgcgtt tttcagcgat cttacgagag
    31921 aggtgcagtt ccatgttgcc tgtaccttta aactcttcgt aaataacttc gtccatttta
    31981 gaaccggtat cgataagcgc cgtcgcgata atggtcaggc tgccgccctc ttccacgtta
    32041 cgcgccgcac cgaagaagcg tttcggacga tgaagggcat tggcgtccac accaccggtc
    32101 aacactttac ctgacgccgg aacaacagtg ttgtaagcgc gcgccagacg agtgatggag
    32161 tcgagcagaa tgataacgtc tttcttgtgc tcaaccaggc gtttggcctt ctcgatcacc
    32221 atttccgcaa cctgaacgtg gcgagatgcg ggttcgtcaa aggtagaagc aacaacttca
    32281 ccttttacca gacgctgcat ctcggttact tcttccggac gttcgtcgat cagcagaacc
    32341 atcagcacac aatccgggtg gttgtaagca atgctctgag caatgttctg cagcagcata
    32401 gttttaccgg ctttcggcgg tgccacaatc agaccacgct gaccacgacc gataggtgat
    32461 gccagatcca gtacgcgagc ggttaaatct tccgtagaac cgttaccacg ttccatacgc
    32521 agacgagagt ttgcgtgcag cggggttaag ttctcaaaga ggattttgtt gcgggcgttt
    32581 tcaggtttgt cgaagttaac ttcgttaact ttcagcagcg caaaatagcg ttcaccttct
    32641 ttcggcgggc gaatcttacc agagatggta tcaccagtgc ggaggttgaa acggcggatt
    32701 tggctagggg aaacgtagat gtcatcagga ccggcgaggt aggagctgtc tgcggaacgg
    32761 aggaaaccaa atccatcctg caatatctcc agtacgccat caccaaagat atcttcgcca
    32821 ctctttgcgt gctgcttcag gatggcaaaa ataatgtcct gcttacgcat acgagccagg
    32881 ttttccagcc ccatattttc gccgagagtg atcagctcag aaaccggcgt attctttaat
    32941 tcggtaagat tcatagtggt gtgagttctt aaacttgggg taaatctcga acttaatgtt
    33001 gtgaatggta tggcaggatc atccatgcct gtttacggtc atcgactcat gtcttttcgc
    33061 tgcctggtca caggagagta cgcagaactg aaacgacaag acggattgag tgacgagcca
    33121 gaaatctgtt cacttcgcat ttaagataaa acgggaagcg ttggaaatta caagattcaa
    33181 acttaataag gtatgtttaa tacgaagtca acactaagtt agcatgactc acgccgggcg
    33241 tccagttttt aacgacgggg cacccgaaca tgaaattccc ttacgccagg ttagcgtcga
    33301 ggaactcttt caactgacct ttagacagtg cacccacttt ggttgccgcc acttcaccgt
    33361 ttttgaacag cagcagagtc gggataccac ggatgccata tttcggcgca gtgccagggt
    33421 tttgatcgat gttcagtttt gcaacggtca gtttgccctg atattcgtca gcgatttcat
    33481 ccagaatcgg ggcgatcatt ttgcacggac cgcaccactc tgcccagaaa tcgacgagga
    33541 tcgccccgtc cgctttgagt acatccgtgt caaaactgtc gtcagtcagg tgaataattt
    33601 tatcgctcat atataactcc acaggaataa gcctggcgtg ttggtttcgt tgttggtgta
    33661 acattaacca actaaaggtt gactttattt caccggatac gctttcgtaa agcaatagta
    33721 agctgatatt ctaccacact atgagcaaaa cacatttaac agaacagaag ttttccgact
    33781 tcgccctgca tccgaaggtt gtagaagtcc ttgaaaaaaa agggtttcat aactgtacgc
    33841 ccattcaggc actggccctt ccgctgacgc tggcgggtcg tgacgtagcc gggcaggcgc
    33901 aaaccggtac cgggaaaacg atggcgtttc ttacgtcaac gtttcattat cttctctctc
    33961 atcctgcgat tgccgatcgc aaggtgaatc aaccacgtgc cttaattatg gcaccgacgc
    34021 gtgaacttgc cgtgcagatc catgccgacg cagaaccgct ggcggaagct accggcctga
    34081 agctgggtct ggcttacggt ggtgatggct acgacaaaca gctgaaagtg ctggaaagcg
    34141 gcgttgacat tctgattggc actacggggc gtttaattga ctacgccaag cagaaccaca
    34201 ttaacctcgg tgccattcag gtggtggtac tggacgaagc cgatcgcatg tacgatctgg
    34261 gctttattaa agatatccgc tggctgttcc gccgtatgcc gcctgcaaac cagcgcctca
    34321 acatgctgtt ctccgccacg ctttcgtacc gggtacgtga actggcgttc gagcagatga
    34381 acaatgccga atatattgaa gtggaaccgg aacagaaaac gggccaccgt attaaagaag
    34441 agcttttcta cccttctaac gaagaaaaaa tgcgtttgct gcaaacgctg atcgaagaag
    34501 agtggccaga cagagcgatt attttcgcca ataccaaaca ccgttgtgaa gagatctggg
    34561 gccacctggc ggcagatggt catcgtgtcg gtttgttgac aggcgatgtc gcgcagaaaa
    34621 aacgtctgcg tattcttgat gaatttaccc gtggcgatct ggatattctg gttgccaccg
    34681 acgttgccgc gcgtggtttg catattccgg cagtgacgca cgtctttaac tacgatttac
    34741 ccgatgactg tgaagattac gttcaccgta ttggtcgtac aggtcgcgca ggcgcaagcg
    34801 gtcactctat cagcctggcg tgtgaagagt atgcattgaa tttgcctgct attgagacct
    34861 atattggtca ctcaattccg gtaagcaaat acaatccgga cgcattgatg accgatctgc
    34921 caaaaccgct gcgcctcacg cgcccacgta caggcaatgg tccgcgtcgt actggcgctc
    34981 cgcgtaatcg tcgtcgttca ggttaaataa aatgtcatac tcaaaatcat tcaaactgta
    35041 tcaagatggc aagagaatga atcccgatga gcatactgta gtaagtgatt caggtgaaag
    35101 aatgcagcca acacagagac agattgaagg atgaagagta tgggttccac ctcgtcgctg
    35161 tatgcagcca ttgatctcgg ttcgaatagt tttcatatgc tggttgtgcg cgaggtggct
    35221 ggaagcatcc agacgctgac gcgaataaaa cgcaaagtgc gtctggctgc tggcctgaat
    35281 agcgaaaatg ccctgtctaa tgaagcaatg gagcgcggtt ggcaatgtct gcgcctgttt
    35341 gctgaacgtc tgcaagatat ccctccctcg caaattcgcg ttgtcgctac ggcgacatta
    35401 cgtcttgccg tcaatgcggg tgattttatt gccaaagcgc aggaaatcct cggttgtccg
    35461 gtacaggtga tcagcggtga agaggaagca cgtctgattt atcagggcgt tgctcacaca
    35521 actggcggtg ccgatcagcg cctggtggtg gatataggcg gtgccagtac tgaactggta
    35581 accggcacgg gtgcacaaac cacctcgttg ttcagcctgt cgatgggctg cgtcacctgg
    35641 ctggaacgct attttgccga tcgtaatctg gggcaggaaa attttgatgc tgcagaaaaa
    35701 gcggcacgcg aagtgttacg tccggttgcc gatgaattac ggtatcacgg ctggaaagtg
    35761 tgcgttggtg cttccggcac cgtgcaggcg ttacaggaaa tcatgatggc acaggggatg
    35821 gatgaacgca ttaccctgga aaagttgcag caattgaaac agcgagccat tcattgcggt
    35881 cggctggagg aactggagat tgacgggctg acgctggaac gtgcgttagt gttcccgagt
    35941 ggtctggcga tcctgatcgc catttttacc gaactgaata ttcagtgtat gaccctggcg
    36001 ggcggtgcgc tgcgtgaagg cctggtctac ggcatgttgc atctggccgt cgagcaggat
    36061 attcgcagcc gtacgctgcg taatattcag cgccgcttta tgatcgatat tgatcaggcg
    36121 cagcgcgtag ccaaagttgc ggctaacttc ttcgatcagg tggaaaagga atggcatctt
    36181 gaagcaataa gccgcgattt gctcatcagc gcctgtcagc ttcatgaaat cggcctgagc
    36241 gttgacttta aacaagcgcc gcaacacgct gcttatctgg tgcgtaatct ggatcttccc
    36301 ggttttaccc ccgcacagaa aaaattgctg gcgacgctac tgctcaacca gactaatccg
    36361 gtcgatctct catcgctgca tcagcaaaat gccgtaccgc cgcgcgtcgc agaacaactc
    36421 tgccgtttac tccgcctggc catcattttt gccagccgtc gccgtgacga tctcgtgcca
    36481 gagatgacat tacaggctaa ccatgaactg ttgaccttga cgcttccgca aggttggcta
    36541 acccaacatc cgctgggtaa agagattatt gctcaggaaa gccagtggca gagctatgtc
    36601 cactggccgc tggaagtgca ttaatgagta agtgccggat gcgatgctga cgcatctttt
    36661 ccggccttga ttatttccct cgttttgccg ccatcatcgc tttcagattc gccagatggc
    36721 tttgcccttt ctgcatccgt tcttcggcgc tgaccacttt gcgctcctgt tcccaaatca
    36781 gatcatcctg cggcagctcc agcaaaaagc ggctcggctc cgggcgcacc agctcgccgt
    36841 actgacggcg ttctttacac agcgtaaagg tcaattcctt ctgggcgcgg gtaatgccga
    36901 cataggccag ccgccgctcc tcatcgatat tatcttcatc gatgctgctc tggtgcggca
    36961 aaaacccttc ttccataccg accatgtaga cataaggaaa ctccagccct tttgacgcgt
    37021 gcagagtcat cagttgcacc tgatccagct cttcttcact ctcaccacgc tccatcatgt
    37081 cgcgcaaagt aaagcgcgtc accacctggg tgagcgtcat cggctcatcc agttcgctgc
    37141 cttccagcat ctccgtcatc cagctaaaca gttggttgac gttcttcatg cgcatttcgg
    37201 cggctttcgg gctgggcgat gtttcgtaca gccaggattc ataatccatg ccgtggatca
    37261 gatcgcgcac cgcggcaatc ggctcccgct ccgccagacg ctggatttct gccaaccagt
    37321 gagtaaagcg ggtcaatgct tcataaccac gtccgctaag cgtctgactc aggcccatat
    37381 caaagctggc ggtaaacatg cttttattgc gcgtcatcgc ccactcaccc agctttttca
    37441 gcgtcgccgg gccaatctct ctcttcggcg tgttaacgat acgcagaaat gcgctgtcat
    37501 cgtccgggtt agtcagcacg cgcagataag ccagcaagtc cttgatttca ggacgagaga
    37561 aaaacgacgt accaccagaa attttgtacg ggatgcggtt ttgcatcagg aatttttcaa
    37621 acactcgcga ctgatggtta ccgcgataaa gaatagcgta atatttgtac tgcgttttat
    37681 tgacgaagtg atgcgcaatc agttcaccag tgacgcgctc ggcttcatgt tcttcgttat
    37741 tcgcgcttaa aactttaagc tctgtgccgt aacccagttc ggagaacaga cgcttctcaa
    37801 agacgtgcgg gttattggcg atcaggatgt tcgccgcttt caaaatacgt ccggaagagc
    37861 ggtagttctg ctcaagctta atcaccttca gcgccggaaa atcctgactc agcagcacca
    37921 ggttttgtgg acgtgcgccg cgccaggagt agatcgactg gtcgtcatcg cccaccacgg
    37981 taaagcgcgc gcggctgccc accagcagtt tcaccagttc atactggctg gtgttggtgt
    38041 cctgatactc atccaccagc agatagcgaa ttttgttctg ccagcgctca cggacttctt
    38101 cattgcgttg cagcagcaac gtcggcaata aaatcagatc atcgaagtcg agaacattgc
    38161 acgctttcag gtgtgcatca tacagcccat aacaatgggc aaaaatacgg tcccgctcac
    38221 caatcgcact ggctgccgcc tgggacggtg ttttgagatc gttcttccag ttagagatgg
    38281 tcgaaatcaa ctgttgcagg agaactttgt catcttcaat cagcccttcg gtcaactctt
    38341 tgagcaacgc cagctgatcg gtatcgtcaa acagcgaaaa gttcgccttc atccccagcg
    38401 ccgcatactc acgcttgatg atatccagcc ccagcgtgtg gaaggttgag atcatcagcc
    38461 cacgcgcctc tttgcgaccc agcgtctgtc cgacacgctc tttcatctcg cgcgccgcct
    38521 tattggtaaa ggtcaccgcc gcgatatgtc gcgcctgata accgcaaccg cggatcagat
    38581 gggcgatttt attggtgatc acacgagttt taccggaacc cgcgcccgcc agcaccaggc
    38641 aggggccggt aacgaattcg acagcttgtt gttggccggg gtttagacgc ataggtgtat
    38701 tgctcaatct tcgaacgggg ggaggattgt agcagaatcg catttgaatg tgaccgcgtg
    38761 taagccgcta accggttggc tgcggatgtt taaatcagag agggaagacc gaaaccggag
    38821 ttcatcgtga atccattaga cttagaaaat atcgggtcgt aacgttgtgt tattactttt
    38881 t
//
LOCUS       DAJVUJ010000016        37821 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.016, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000016 DAJVUJ010000000
VERSION     DAJVUJ010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37821
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.016"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..381
                     /locus_tag="OQQ48_000807"
     CDS             <1..381
                     /locus_tag="OQQ48_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1951664.1"
                     /translation="LICSNKHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNSHRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNESF"
     gene            528..2045
                     /locus_tag="OQQ48_000808"
     CDS             528..2045
                     /locus_tag="OQQ48_000808"
                     /inference="COORDINATES: protein motif:HMM:NF014273.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HCS1951665.1"
                     /translation="MSIYKIPLPLNILEAARERITWTLNTLPRVCVSFSGGKDSGLML
                     HLTAELARQMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFCFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQSLCNPLYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEDDRIKAINEIRMAIHQ
                     VSPFREEPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLQKSIEIDGFTQPIVVTHTDK
                     NAMEIVDGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQIT
                     AMSEIVRELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(2046..3195)
                     /gene="ybdL"
                     /locus_tag="OQQ48_000809"
                     /pseudo
     CDS             complement(2046..3195)
                     /gene="ybdL"
                     /locus_tag="OQQ48_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308666.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
     gene            3304..4392
                     /gene="hcxA"
                     /locus_tag="OQQ48_000810"
     CDS             3304..4392
                     /gene="hcxA"
                     /locus_tag="OQQ48_000810"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HCS1951666.1"
                     /translation="MPHNPIRVVVGPANYFSHPRSFNHLNDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(4402..4599)
                     /locus_tag="OQQ48_000811"
     CDS             complement(4402..4599)
                     /locus_tag="OQQ48_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCS1951667.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(4781..6886)
                     /gene="cstA"
                     /locus_tag="OQQ48_000812"
     CDS             complement(4781..6886)
                     /gene="cstA"
                     /locus_tag="OQQ48_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HCS1951668.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPKRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLALFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(7067..7480)
                     /gene="entH"
                     /locus_tag="OQQ48_000813"
     CDS             complement(7067..7480)
                     /gene="entH"
                     /locus_tag="OQQ48_000813"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HCS1951669.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(7483..8229)
                     /gene="entA"
                     /locus_tag="OQQ48_000814"
     CDS             complement(7483..8229)
                     /gene="entA"
                     /locus_tag="OQQ48_000814"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HCS1951670.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAGQVAQVCQRLLAETERLDVLINAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(8229..9086)
                     /gene="entB"
                     /locus_tag="OQQ48_000815"
     CDS             complement(8229..9086)
                     /gene="entB"
                     /locus_tag="OQQ48_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HCS1951671.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFELQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPVPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(9100..10710)
                     /gene="entE"
                     /locus_tag="OQQ48_000816"
     CDS             complement(9100..10710)
                     /gene="entE"
                     /locus_tag="OQQ48_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HCS1951672.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GELQLSYRELNQAADNLACTLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLHNDSGE
                     HNLQDAINHPADGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLSQWLASRASA"
     gene            complement(10720..11895)
                     /gene="entC"
                     /locus_tag="OQQ48_000817"
     CDS             complement(10720..11895)
                     /gene="entC"
                     /locus_tag="OQQ48_000817"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HCS1951673.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKVQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QASARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVLASSPLGEWRETGVKLSTMLNVFGLH"
     gene            12270..13226
                     /gene="fepB"
                     /locus_tag="OQQ48_000818"
     CDS             12270..13226
                     /gene="fepB"
                     /locus_tag="OQQ48_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HCS1951674.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPTGLNASQSQGKRHDIIQLSGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(13230..14480)
                     /gene="entS"
                     /locus_tag="OQQ48_000819"
     CDS             complement(13230..14480)
                     /gene="entS"
                     /locus_tag="OQQ48_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HCS1951675.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWLVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAEIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            14591..15595
                     /gene="fepD"
                     /locus_tag="OQQ48_000820"
     CDS             14591..15595
                     /gene="fepD"
                     /locus_tag="OQQ48_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HCS1951676.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            15592..16584
                     /gene="fepG"
                     /locus_tag="OQQ48_000821"
     CDS             15592..16584
                     /gene="fepG"
                     /locus_tag="OQQ48_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HCS1951677.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLETSQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            16581..17396
                     /gene="fepC"
                     /locus_tag="OQQ48_000822"
     CDS             16581..17396
                     /gene="fepC"
                     /locus_tag="OQQ48_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HCS1951678.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIKHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTHWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLERTAPSTANS"
     gene            complement(17393..18526)
                     /gene="fepE"
                     /locus_tag="OQQ48_000823"
     CDS             complement(17393..18526)
                     /gene="fepE"
                     /locus_tag="OQQ48_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HCS1951679.1"
                     /translation="MSSLNIKQGSDAHFSEYPLASPSSNEIDLLNLIEVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSSAVITPAEAIQWQDLEKTFTKLRVLDLDVNIDRGG
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSKEAQTVLSGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANVNDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
     gene            complement(18741..22622)
                     /gene="entF"
                     /locus_tag="OQQ48_000824"
     CDS             complement(18741..22622)
                     /gene="entF"
                     /locus_tag="OQQ48_000824"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HCS1951680.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVDGLAQADTLRMRFTEDNGEVWQWVDDALTFELPEIIDLRTNIDPHGTAQALMQ
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGR
                     SASADILRLKMEFTDGEFRQLATQLSGVQRTDLALALTALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYQFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     ADVPDLTNLCYNAPLKPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYLLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQSCATLKQVFCSGEALPADLCREWQQLTSV
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFVPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPLLPLSANGKLDRKALPLPELKAQAPGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQVARQVTPGQVM
                     VASTVAKLATIIDAEEDSTRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYI
                     DPQWSIIGIQSPRPNGPMHTAANLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFVKIGPIIRATLNR"
     gene            complement(22619..22837)
                     /gene="ybdZ"
                     /locus_tag="OQQ48_000825"
     CDS             complement(22619..22837)
                     /gene="ybdZ"
                     /locus_tag="OQQ48_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HCS1951681.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQESCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            complement(22840..24042)
                     /gene="fes"
                     /locus_tag="OQQ48_000826"
     CDS             complement(22840..24042)
                     /gene="fes"
                     /locus_tag="OQQ48_000826"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HCS1951682.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     PIKRVWVYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKNSRRVWIFTTGDATAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            24285..26525
                     /gene="fepA"
                     /locus_tag="OQQ48_000827"
     CDS             24285..26525
                     /gene="fepA"
                     /locus_tag="OQQ48_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HCS1951683.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGNTQNTNSDAYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGANTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATRDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            26700..27320
                     /gene="entD"
                     /locus_tag="OQQ48_000828"
     CDS             26700..27320
                     /gene="entD"
                     /locus_tag="OQQ48_000828"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HCS1951684.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKAEHLAGRIAAIYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(27442..27594)
                     /gene="hokE"
                     /locus_tag="OQQ48_000829"
     CDS             complement(27442..27594)
                     /gene="hokE"
                     /locus_tag="OQQ48_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HCS1951685.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            28047..29165
                     /locus_tag="OQQ48_000830"
     CDS             28047..29165
                     /locus_tag="OQQ48_000830"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCS1951686.1"
                     /translation="MPLPDFHVSEPFTLGIELEMLVVNPPGYDLSQDSSILIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQVTVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            29231..29479
                     /locus_tag="OQQ48_000831"
     CDS             29231..29479
                     /locus_tag="OQQ48_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HCS1951687.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            29544..29912
                     /locus_tag="OQQ48_000832"
     CDS             29544..29912
                     /locus_tag="OQQ48_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCS1951688.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            30006..30658
                     /gene="nfsB"
                     /locus_tag="OQQ48_000833"
                     /pseudo
     CDS             30006..30658
                     /gene="nfsB"
                     /locus_tag="OQQ48_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
     gene            30766..32013
                     /gene="ybdG"
                     /locus_tag="OQQ48_000834"
     CDS             30766..32013
                     /gene="ybdG"
                     /locus_tag="OQQ48_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HCS1951689.1"
                     /translation="MQDLISQVGDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGT
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESILNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPAGNDIRSLAGAFKQ"
     gene            complement(32081..33457)
                     /gene="pheP"
                     /locus_tag="OQQ48_000835"
     CDS             complement(32081..33457)
                     /gene="pheP"
                     /locus_tag="OQQ48_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HCS1951690.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYICSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNEVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSHRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(33559..36702)
                     /gene="cusA"
                     /locus_tag="OQQ48_000836"
     CDS             complement(33559..36702)
                     /gene="cusA"
                     /locus_tag="OQQ48_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HCS1951691.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQVGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMPFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKNDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIVEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGGAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(36714..>37821)
                     /gene="cusB"
                     /locus_tag="OQQ48_000837"
     CDS             complement(36714..>37821)
                     /gene="cusB"
                     /locus_tag="OQQ48_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HCS1951692.1"
                     /translation="RKILFWYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVR
                     IDPTQTQNLGVKTATVTRGPLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVG
                     DKVQKGTPLLDLTIPDWVEAQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLI
                     ATQKIQTRFTLKAPIDGVITAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWL
                     VKDASQFTLTVPARPDKTLTIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWL
                     QLNTASEPMLLIPSQGLIDTGSEQRVITIDADGRFVPKRVAVFQASQGVTALRSGLAE
                     GEKVVSSGLFLIDSEANISGALERMRSESATHAH"
BASE COUNT         9073 a        10549 c         9636 g         8563 t
ORIGIN      
        1 ctaatttgta gtaataagca tccgcgaatt actgacaata gcacttatga acaaattatg
       61 gcagaagagt tcacccaatt aatatccaaa tcagcaggag ttgatgatat tcaaatggaa
      121 atagacgaga agtttatgaa tcgcaaaatt tcatttcgtg gttcctcttt gctgacaata
      181 attaatagca ttgcagttac agatttactg ggaatagtcc cctatgaact ttataactcc
      241 caccgtgact ttcttaattt aaaagagata aaactagaac atccgttacc ttcaataaag
      301 ctttacattt cctataataa atcttcttta aataatttgg tgttctcccg atttattgac
      361 cgtctaaatg agagtttttg atatataact acagacagct actataactt tcatctattt
      421 attcacagcg cgaatgaaaa ataaaacact ctgctttaca gtgtttactt tataattaac
      481 agtcttatct cactgttcac cctgtaacgt tcagagagcg tcttcagatg tctatttata
      541 aaattcctct tccgctcaat attctggaag ctgcacgaga gcgtattacc tggacactca
      601 acaccctacc ccgcgtttgt gtctcatttt ctggcggcaa agactctggt ttaatgctgc
      661 atctgacagc cgaacttgcc cgacaaatgg gcaaaaaaat ctgcgttttg tttatcgact
      721 gggaggcgca attctcttgc actattaact atgttcagtc cctgcgcgag ttgtacaccg
      781 atgtcatcga agagttttac tgggttgcgc tcccgcttac gacgcaaaat tccctttcac
      841 aataccaacc cgaatggcag tgctgggaac ctgatgtcga atgggtgcgt cagcccccgc
      901 aagatgcgat aaccgatcct gactttttct gcttttacca gccaggcatg accttcgaac
      961 aatttgtacg tgagtttgcc gaatggtttt cacaaaaacg tccggcggcg atgatgatcg
     1021 gcatccgtgc ggatgagtcc tacaaccgtt ttgtcgccat cgccagttta aataaacaac
     1081 gttttgccga cgataaacca tggactacgg ccgcaccagg cggtcatagc tggtacattt
     1141 accccattta cgactggaaa gtggctgata tctggacctg gtatgcaaat catcaaagtc
     1201 tctgcaaccc actgtataac ctaatgtatc aggcaggcgt tcctctgcgt catatgcgaa
     1261 tttgcgaacc ttttggcccg gagcaacgac aaggattatg gctctatcac gttatcgaac
     1321 cggatcgctg ggctgctatg tgcgcacgag tcagcggggt aaaaagtggc ggcatttacg
     1381 ccggacatga caatcatttc tatgggcatc ggaaaatcct caagccagaa catttagact
     1441 ggcaagaata tgcattattg ctgctcaata gcatgccgga agatgatcgc atcaaagcga
     1501 ttaatgaaat ccgcatggcg atccatcagg ttagcccctt tcgtgaagag ccagtggatt
     1561 gcgttctgtg ggttaaaaac agccaactta tgcctaatga ttacaaccca aacaacgtgg
     1621 caccacccga aaaaaaactg ctgcaaaaat ccattgaaat agatggtttt acccaaccca
     1681 ttgtggtcac acatacggat aaaaatgcca tggaaatcgt ggatggtttt caccgccatg
     1741 aaattggtaa aggctcatca tcattaaaat tacgcttaaa aggttatttg ccggtgacct
     1801 gtctggaagg tacgcgtaat caacgcatcg ccgcgactat tcgtcataat cgcgctcgtg
     1861 gacgccatca aatcaccgcc atgtcggaaa tcgtccgtga acttagccag ttgggatggg
     1921 acgataataa aattggcaaa gagctgggaa tggacagcga cgaagtgctg cgcctgaagc
     1981 aaattaacgg cctgcaagag ttgtttgccg accgtcaata ttcccgcgcc tggacggtta
     2041 aatagctaaa gctggcgcag gcgttcagct gctgccagca acgtcgattc cttcttggca
     2101 aaacagagac gaatcagttt atgtgggaag ggatcggcgc aaaacaccga cagcggaatc
     2161 gccgccacgc cgtgctcccg cgtcagccac tggcaaaact caacatcatc cagggtagaa
     2221 acctcgctgt aatccaccag caaaaagtat gtaccttcac acggtaaaat ctccagccgg
     2281 ctttcattta aagcattcac cagaatatcg cgcttctggc gataaaagtc cggtaacgca
     2341 agataatgct caggttctgc acgtagcata tcagcaagcg ccagctgtgc cggggtattc
     2401 accgaaaagg tcagatactg atgtaccttg cgaatttcgg cgctgatggg cgctggcgca
     2461 acacaataac ccactttcca gccggtcata tgatagatct tgccaaatga agaaaccgcc
     2521 actgcccgct cacgcagctg cggatgcgcc agcacactgg catggccctg ttgtgaaaag
     2581 ttgatgtgct cgtagacttc atcgctaatg acaaaaatct cgtgcccggc gatcgcctgc
     2641 cacaaagcgg cgaaatcagc ctgctgccag acagttgcac tggggttatg cggagtgttg
     2701 aggatcacca gtctggtgcg ctcgcttaat aatgcggcaa attcctgcca gtcaaggtgg
     2761 ttgcagtgcc atacgcttca ctattccccc agaaagcgcg atggcggggg cgtaactgtc
     2821 atagctggga tcaaaacaaa tcacttcatc gccattgcgc accagtgcgg taatcgccgc
     2881 gtataacgct tccgtcgccc ctgccgttac ggtgatatcg ctatcggcat ctggttgata
     2941 gccatacaaa cgttccgttt tctgagcaat cgcctcgcgc aaggcctgca cgccggtcat
     3001 gggcgcgtat tggtttgccc cctgggcaac gtggtacgcc agccgctcct gtaaatagcg
     3061 cggaccatca aaatcaggaa agccttgcga caggttaatc gcctggtgtt gctgcgccag
     3121 cgcgctcatc tgggtgaaaa tagtggtgcc aagttgtgga agtttgcttt gtggaatcag
     3181 agggttattt gtcattttta ttgtacctga cgaagttgcg tgttgaaggc actataacac
     3241 gatgctagta tttggcaatc aagacgttta gatgtctaaa taaaacaata aggacaacac
     3301 aacatgcctc acaatcctat ccgcgtggtc gtcggcccgg ctaactactt ttcacatcca
     3361 agaagtttca atcacctgaa cgattttttc actgatgaac aactttctcg cgcggtgtgg
     3421 atctacggca aacgcgccat tgctgcggcg caaaccaaac ttccgccagc gtttggactt
     3481 ccaggggcaa agcatatttt gtttcgtggt cattgcagcg aaagcgatgt acagcaactg
     3541 gcggctgagt ccggtgacga tcgcagcgtg gtgattggcg tcggcggcgg cgcactgctc
     3601 gacaccgcga aagccctcgc ccgccgtctc ggtctgccat ttgttgccgt tccgacgatc
     3661 gccgctacct gcgccgcctg gacaccgctc tccgtctggt ataacgatgc cggacaggcg
     3721 ctgcattatg agattttcga cgacgccaat tttatggtgc tggtggaacc ggagattatc
     3781 ctcaatgcac cgcaacaata tctgctggcg gggatcggtg acacgctggc gaaatggtat
     3841 gaagcggtgg tgctggctcc gcaaccagaa acgttgccgc taaccgtgcg gctggggatc
     3901 aataatgcgc aggccattcg cgacgtcttg ttaaacagta gcgaacaggc acttgccgat
     3961 cagcaaaatc agcagttaac gcaatcattt tgcgatgtgg tggatgctat tattgctggt
     4021 ggtgggatgg tcggtggtct gggcgatcgt tttacgcgtg tggcggcggc tcatgccgtg
     4081 cataacggtc tgactgtcct gccgcaaacc gagaagtttc ttcacggcac caaagtcgct
     4141 tacggaattc tggtgcaaag cgccttgctg ggtcaggatg atgtgctggc gcaattaacc
     4201 ggggcgtatc agcgttttca tctgccgact acgctggcgg agctggaagt ggatatcaat
     4261 aatcaggcgg agatcgacaa agtgattgcc cacaccttgc gcccggtgga gtccattcat
     4321 tacctgccag tcacgctgac accagatacg ttgcgtgcag cgttcgaaaa agtggaatcg
     4381 tttaaagcct gacgtgcaaa tttagcagca gcgcgcgccg ccttttccac cgtagcgcgc
     4441 gtcctgccgc tcacggaaaa actcctcgta ggtcatcggc gtttgatcgg gatggttaac
     4501 ccgcatatgt tcgacatagt tgtcgtaatc aggcatacca atcatcagct tcgccgcctg
     4561 acctaaatat tttccggctt ttgccagtga atcaaacatc ttggcctccg ctaaccatgt
     4621 tgctgaaaga gtgccggatg cggcgtgaac gctttatccg gcctacaaat gctatcaacg
     4681 cactcggtca gtcccctcgc cccatcgggg agagggttag ggtgaggggg aaccctcggc
     4741 actatgccaa cattcgccaa catcccccct cactctgact ttagtgcgcg cctttcgcct
     4801 gcgctacgat ctcctcgaca ttttccggca tcggctcata cggcgtttct ttcgccgttg
     4861 gcttcggatc tttcaatgcc gcaagtgccg tcttaatcga gaacagtgcc agaaccacca
     4921 cgaccaccat aaagaagatg gttaacccgg catccagacg gttgttgaac accagttgcg
     4981 ccagctgtga ctcagtatac tgcgacggaa tattgccgct gtcgatcatt gcctggaact
     5041 tgttagcaat ggccaggaag ccgactttcg catccgggct aaacgctttc tgccagccag
     5101 cggtcagggt acaaatcagc agccaggccg ttggtaccag cgccacccag gcgtaacgtt
     5161 gacgtttcat cttgaacaac accacggcac agagcatcag cgccatccct gccagcatct
     5221 ggttggcaat accaaacagc ggccacagag tgttaatacc gcccagcgga tcgaccacgc
     5281 cctgatggag gaagtagccc cacgccagta cgcacagcgc cgttgccagc aggttagcag
     5341 gcagtgaatc ggtccgcttc aggccaggag acaccacgcc cagcagatcc tgcaacataa
     5401 agcgcgcagc acgcgtaccc gcatctaccg ccgtcagaat aaacagagct tcaaacagaa
     5461 tggcgaagtg ataccagaac gccacatcca tcataccacc cagcgcaccg tgcaggatgt
     5521 aagccatccc caccgccaac gtcggcgcac cgcccgcacg ggaaatgatc gactgttcac
     5581 ccacttcgct ggcaatctgg ttgagcgtat ctggtgtaat gctaaagccc cagctactca
     5641 ccacctgcgc ggcagaagcg accacatccg ccgtccctgc tggagccagc accgccattg
     5701 ggctgttcat ggcaaagtaa acgcccggat cgatgataca ggcggaaacc agcgccataa
     5761 tcgccacgaa ggattccatt aacattccac cgtagccgat aaaacaagcc tgcccttcgt
     5821 tcgccagcat cttcggcgtg gtgccggaag agatcagcgc atggaagcca gacaccgcgc
     5881 cacaggcgat ggtgataaac aggaacggga acaggttacc ggtccatacc gggccagtgc
     5941 catcgacaaa tttggtcagc gcaggcatgg tcagcgtcgg gcgcataatc aaaatgccta
     6001 ccgccagacc gacgatcgtc ccgattttca ggaaggtaga gaggtagtca cgcggggcca
     6061 gcagcaacca caccggcagc accgccgcca caaaaccgta acccaccagc atccaggtca
     6121 gctgcacgcc ggtaaagtca aagtacggtg cccaggtcgg actttctgcc acccagccgc
     6181 cagagataat ggcgaaaatc aggaatacca gaccgatgac cgacacttca ccaatacgcc
     6241 ccggacgcag atagcgcagg tagatcccca taaacagcgc cagcggaatg gtgaacgcaa
     6301 cggtatacgt tccccacggg ctatgagtca gggctttcac cacgatcatc gccagtactg
     6361 caaggataat gaccatgatc ataaagcagg ccaccagcgc tatcaccccg gcggttggcc
     6421 ccatctcttc tttgaccagc tcacccagcg atcgaccgtc acggcgcgta gaaacaaaca
     6481 gcaccatgaa atcctgcacc gcaccggcga gaaccacccc ggcgagtagc cagatcatcc
     6541 ccggcaggta gcccatttgc gctgccagca ccggcccaac caacggacct gctccggcga
     6601 tggccgcaaa atggtgaccg aacagcactt tcttgtccgt cggcacatag tccagcccgt
     6661 cgttatggcg caccgctggc gtcatacgct tcgggtcaac cgccagcaca tttttggcga
     6721 tatacaaccc ataaaaacgg taagcgatca gatagataca gaccgacgcc accacaatcc
     6781 acagcgcgtt gatctgttcc ccacgattta aagcaatgta tcccagagca aatgctccca
     6841 ttacagagag cactgtccag acgaggtatt tccctgattt gttcatagtt gttatccgtg
     6901 tgcgtgtcca tagagatgtt acattttgtt tctataacat ctttgtacga tttaacaaca
     6961 cggtaactat gtaaatcgga gttgaaacca atatttaact taacaatgtt aactcgatca
     7021 ctccgttaac cgagttttcc tgcaatctca tttaattctg tcgggctcat cccaaaactg
     7081 ccgtacccag ccgacaagtg cagcaacgcc gcccctgttc atcgaaaacg acgatttccc
     7141 agctctgatt ttgccgccca agatgcagcg gctggcagac gccgcgtacc ttgccttcag
     7201 acaccgggcg atgatgggtg gcgttaagtt ccgtgcccac cacacactgc ccgtcacggg
     7261 tcatcataaa tccggccatc gatcccagcg tttcagccag cgccgccgac gcgccgccat
     7321 gcagcaggcc aaacggctga tgagtacggg tatcaaccgg catttcggct tccagcacat
     7381 catctcccag acgggtatac acaattccca gatgcgccac cattgtgtta tcgctggtgg
     7441 cgttcagttc gtcgagcgtt aaatggcgtt tccagatcat gcttatgccc ccagcgttga
     7501 gccgccatcg accacaatat cctgtagggt gatatggctg gcgaggtcag aggcgaggaa
     7561 caaaatcgtg ttggcgatct cttgtggacg ggcgattttc cccagcggaa tgccgagttt
     7621 aaactgctcg ccaaagccgc gaatacgctg ttcttcggcg tcatcgctca cccacagcgt
     7681 gcgttgcata tcggtgtcgg tggagccagg cgaaaccaca ttacagcgca cgccgctacc
     7741 cgccagttcc agcccgacgc tcaacgccag gcttttcagc gccgctttcg atgcgccata
     7801 agcactcata ccaatacgcg gcgtgtgtgc ggcgtcggac gccacagtga caatcgcccc
     7861 gccccgctga cggcgaaact ggttcatggt ttgctggaac aggttaaacg caccgccgac
     7921 gttaaccgca aaagtctgct gccagtcctc tttactgagc tgatcggtcg cgcccatgcg
     7981 caaaattccc gccgcattga tcagcacgtc cagtcgctcc gtttcagcta acagtcgctg
     8041 acatacctgc gccacctgcc cggcgtcggc aacatccatt acttcggtcg caaagggata
     8101 ttgctcctga gtgaacgctt gatcaaaacc tgtaactttc gctcccgcct caacaaacgc
     8161 cagcgccgtg gcgtagccga tacctttacc tgcgccggtt acccagacat ttttaccgct
     8221 gaaatccatt atttaacctc gcgggagagt agcttccacc aggcgtcgat ggtcgggttt
     8281 ttcgccagca tgacaaagtc gatatcacca tgcactttgc gccagcgcgc cgccagcgcc
     8341 atcatgcgca ccgaatccag accgtagtcg atcaggttgt cgtcatcgaa cggttcatcg
     8401 gactcgtcca gcaacggcag gataacctca cgcagcgccg ctttgctggc ggggacaggt
     8461 gctggcagta attcttcagt catcaccacc cggccagacc gcccggccac atatttcagc
     8521 gacatcaaat gctcgtcacg gctgaaatcg gccagcgcat ccgccaccat aaacggttta
     8581 atatcgcgca taaatgcgtc ggttgcggtg gtcatacagc caatgtgggc atataccccg
     8641 gtaataatca gctggttacg tccactctct ttgagcattt gctccagcgg agaacgatga
     8701 aacgcgctgt agcgccactt caccagcacc gtgtcgtcgg catctggcgt caggcgatcc
     8761 accacctttt gctgttccgg cgaacgggtc aggcccggcc cccacatatc attcaacagc
     8821 gcccgatctt catcgctctg ctctttcggc tgggcggtgt aataaactgg gatattgtgc
     8881 tgtttgcagt agtcgcgcag cgcagcaata ttcgcgatca cctgctccat catcgggcag
     8941 ttctcgcccc agaagctgac aaaatagtcc tgcatatcat ggattaacaa cgcggcacgt
     9001 tgcagttcaa aggcccagtc aactttattc tgcggaatat cgtgagactc cggcagtgcg
     9061 taagcctgta attttggaat agccatcgtg ttctctcctt caggctgatg cgcgtgacgc
     9121 cagccactga cttaattgtt ttttatcgac tttcccgacc gccgtcagcg gaagtgaatc
     9181 tacacactcc acgcgatccg gtaatttaaa ttcggcaata ccctgttcac gcaggaaacg
     9241 acgcacctgc accgcgcgca gcggctcttt taccaccaga taagcacagc ttttttcgcc
     9301 catcagctca tcttccatgc tcaccagtgc ggcatagatc accgccggat ggcgcagcag
     9361 caggttttcg atctcttcgg cagcgatctt ctcgccgcca cggttgatct gatctttctc
     9421 gcgcccctgc acggtgatgt aaccctctgg atcaatagag atcagatcgc cggaacagta
     9481 aaaaccgttg gcatcaaagg cgctggcatt gtgctgcgga cttttgtaat aaccgcggaa
     9541 ggtgtacggc ccgcgcgtca tcaggcgtcc gacttcccct tgcggcagtg gatttccttc
     9601 ggcatcggca acccacactt catcgtccgg acacattggg taaccctggg tatggataat
     9661 tttctccgcg ctatcatcaa gacgggtgta gttcaccagc ccttccgcca tgccaaacac
     9721 ctgctgcaac tggcagccaa tctcagcggg aatacgcgcc gcaagggtgg cagaaagacg
     9781 tgcgccgccg acctgtaaca gtttcagcga ggcaagctgc gcccggcttt cgccttcagc
     9841 cagcgcctgc aaccacaggc tgactgccgg cggcaccagc gcggtgacgt tcacctgatg
     9901 tttttcaatc aatgggaagc aaagcgtagc gctgggatcg gccgccagaa caaccgttcc
     9961 tccggcaaga aagacgccca gcgatcctgg cgaactcatg gcgtagttat gagccgccgg
    10021 gatcgcgcac aggtagcgcg tctgttgtgt gaactgacaa atctcgacgc tacgacgcac
    10081 gctgtagtag tagtcgttat gagtgcgcgg gatcagcttc ggtgtaccgg tggtgccgcc
    10141 ggaaagctgg aaataggcca cttcatcagc aggtgatggc gtggcagtaa aaccgtctgc
    10201 cggatggtta atcgcatcct gcaagttatg ctcaccgctg tcgttgtgca gttgcaccac
    10261 gcgaatggag gaatgttctg tgacgaaagt attgaggaaa tcatccccgc taaacagcgc
    10321 atgttggcga tcggcaatca gcaatgcggg ttcaatctgg ctggcatagg cgttcagttc
    10381 actacgctga tggctgaaca acgccagcac cggcgcaacg cccagtttca gcagcgcgaa
    10441 aaaggtaata tacaattcag cgacgttacc cagttgtacc agcgcggttt caccaggttt
    10501 aatgccctga cggcgtaaag tacacgcgag gttatccgcc gcctgattca gctcccggta
    10561 actcaactgt agctcgccgt cgataaccgc gatgctgtca ctcgcagcgt ggcgagtcag
    10621 aatgtcggtc agcggcaaat cctgccagta gcctttttcc cgatagcgac gggcaaactc
    10681 ttccggccag cgggtgaatg gaatgctcat cctcgctcct taatgcaatc caaaaacatt
    10741 caacatggta gaaagtttga cgcctgtttc gcgccactca cccaacggtg acgacgcaag
    10801 cacaatcccc gctccggcaa acagacgcac ctgattttcc cgcagcttcg cgcagcggat
    10861 ggtcaccacc cattcgccgt taccttcgct gtcacaccaa cccacaatgc cgccaaacag
    10921 ttcgcggtcg aacggctcca gttcagcaat aacctgagtc gcggcctgat gcgggaaacc
    10981 gctcagcgcg ggggtcggat gcagcagaca ggccagagtc agtgcgtttt cttgcgaatt
    11041 cgctttacct tcaaagggag ttgcgagatg ccacagcgtc ggcgtggtaa tcaattgtgg
    11101 agaggaggga acgtgtaact cactactgcg ttcgcgcagt acctctttca tcgcctgagt
    11161 caccagttca tgttcatggc gatctttttc tgacgccagc agacgattac ccgcttcgcg
    11221 atcgagcact tcatccggct gacgacgcgc ggaaccggct aacggaatgg agctaaaacg
    11281 ctcgccgtct ttacgtagca gcagttccgg gctggccccc agcaggacgc caccatcagc
    11341 cagcgggaca tggaagttgt aactaaccgg gttttgcgca atcaaccgtt ccagcaatac
    11401 gccactatca atggcggcgt cagtggtgat atcaatcaac cgtgacaaca ccactttgtc
    11461 aacctgcggc gtggcggtaa gtgcggcagc gcgggcaacc atctgttcaa acgtggtttg
    11521 ttccggaatt gcctggcgtt ccaccacatt cagcgactgg ctgcgggtga aacggcgggc
    11581 ggaagcttgt ttttcctgac gggagaacga ctgccaggat tcaggaatat acagcgacga
    11641 aggctgacgt ggatcgaagg gaatcgcccc gaccatcacc ggatttttga tgccctgcac
    11701 tttggcatcg gcaaacagcg cggcgagttt ttgctggaag ggactgtcgg gcgaatcccc
    11761 gttcacagcc ggttcatcga agcgggcgaa acatcctgac gtcgtaaaac tgcggtacgg
    11821 cgacataaag aaaaagcgat tgggcgcaag tgttgccatg gtctgctgta cttcctcagc
    11881 cagtgacgta tccatatcat cctccacaaa atgataaagg ctttaataat gattatcatt
    11941 tatattttcg gtcgctaacc taaaagcaaa cgcgcactat gtcaactctt gaggtaacgc
    12001 aatctgcaac tcggggttgc atctggttgt ggttacaatg aaaatgagaa gcattattga
    12061 tggattcgca taagcgcaat gtgatgtcct gcgccgttct tccccctctc ccttccaggg
    12121 agagggctgg ggtgagggtc aatattcgca ccagtgctgg ctgctcccat caccctaacc
    12181 ctctccccaa aggggcgagg gtacggatag tgcgctttgt cgaatttgtc attacgccct
    12241 taaccttatt aataacagga agctgatttg tgagactcgc cccgctctac cgcaacgccc
    12301 ttctattaac aggacttttg ctttccggaa tagccgcagt tcaggccgcc gactggccgc
    12361 gtcagattac tgacagccgg ggcactcata ccctggaaag ccagccgcag cgtattgttt
    12421 ccaccagcgt caccctgacc ggctcactgc tggcgattga tgctccggtg attgccagcg
    12481 gcgcgaccac gccaaataac cgcgtcgcgg atgaccaggg ctttttacgc cagtggagca
    12541 aggtggcgaa agaacgcaaa ctacaacggc tctatatcgg cgaaccgagc gccgaagccg
    12601 ttgccgcgca aatgccggat ctgattttaa ttagcgcaac cggcggggat tcggcgctgg
    12661 cgctgtatga tcagctttcc accatcgccc cgacattaat catcaattac gacgacaaaa
    12721 gctggcagtc gctgttaacg cagcttggcg aaattaccgg gcatgagaaa caagcggcag
    12781 agcggattgc acagtttgat aagcaactgg cagcggcgaa agagcaaatc aaattaccgc
    12841 cgcagccggt cactgctatt gtctataccg ccgctgcaca cagtgccaat ctctggacgc
    12901 cggaatcagc acaagggcag atgctggaac aactcggctt tacgctggcg aaattgccca
    12961 cagggttaaa cgccagccaa agccagggca aacgccatga catcattcag cttagtggag
    13021 aaaatctggc tgcagggtta aatggcgagt cactattcct gttcgccggt gatcagaaag
    13081 acgccgatgc gatttatgcc aatccactgc tcgcgcacct gcccgcagta caaaacaagc
    13141 aggtttatgc gctgggaacc gagacgttcc gcctggatta ctacagcgcc atgcaagtgc
    13201 tggaacgact taaggcgctg ttttaagcat taactgtcgg acgctgtcac ctgcggcggc
    13261 gtctggcgaa aacgtcgcaa ctccaccagc accagcaata acaacacgcc gataatcaac
    13321 aaaccaaaac cgctcgcgct tgcggaagca accggtgtca tcatcgctcc cagaccgcct
    13381 aacagcgccg cgcctatggc atcgcccgtc acgttttgtg ccgtccacaa accgttgatt
    13441 cgccctaaca ttgcttccgg cgtttgcgtt tgcagcattg tatattgcag caacgaactg
    13501 accgcactca gccagccgaa cagcgccaga caaaccacgc ctaaaatcca catcggcatc
    13561 aggccaaata aaccaatggc gaggaacgat cccaacgtgg agagcaacat caacaatcct
    13621 ggtcgcgcgc tatgcgccag tttcccactg gttaatgcgc caatagccgc accgagcggg
    13681 atcgccgcat agagaaaacc aatctctgcc gccgacatct gccagttgtc agccagcgcc
    13741 ggatacagta cccgcaccgc gctcgccatc gtcaataaac cacccagcaa cgcaatcccg
    13801 cccaccagcg ggctggcgag cagaaaacga aatcctgcca gtaatgattt caacggatgc
    13861 tcgcgcggct gcggtggcgg tggcaacgcc ggaaggctta acaacggtag caaggtaata
    13921 aacgtgcccg ccgccgccag cccgtagttc caggctacgc caccggtcgc cagcaataaa
    13981 ccgccaatca tgggcgaaat caccgacccc agacgcacgg tcaacatggt gatcgccccg
    14041 gcctgcatta agttttcacg ccctaccagt gctggtgtcg ccgccaatag cgccgtaacg
    14101 ccaagcgatg cgaaaaaacc atcccataaa ccaagtaaat agattgccag caatgacggc
    14161 tccggcagca gtgcattaag gcacagtcca atgaagccaa tgccacaggt gccgcgcgcc
    14221 agcaaaatca cttttttgcg ctcatagcga tccgccagca caccgccaac catcaggcca
    14281 acaaacatcg cgccgccggt cagcgtcacc gaaagcccca ccagccaggt agaatgcgtc
    14341 atcatctgga tctgcaccgg caccgcgacg ccgagcaaac ccagagacac aattgagata
    14401 aaacgagcga ggaatactgc gcgaaacgcc gggtgcgttt tcaacaggct gaggttaagc
    14461 agccaggatt gtttattcat tacaatgcct tgccatcaaa tattttaaat acctattctt
    14521 aggctgcaca tgctaacata tccaaataag atcgataacg ataattaatt tcattatcat
    14581 ggaagttcgt atgtctggtt ctgttgccgt gacacgcgcc attgccgtgc ccggattgct
    14641 gttattactg attatcgcga cggcattaag cctgctcatt ggggcaaaat cactccccgc
    14701 ttccgtagtg ctggaggcct tctccggcac ctgccagagc gccgactgca ccatcgtgct
    14761 cgacgcgcga ctgccgcgca cccttgccgg tttactggca ggcggcgcgc ttggccttgc
    14821 cggggcgttg atgcaaaccc tcacccgaaa tccacttgcc gaccccggct tgcttggcgt
    14881 gaacgccgga gccagctttg ccattgtgct gggtgcggcg ctgtttggtt actcatccgc
    14941 gcaggaacaa ctggcgatgg ccttcgccgg ggcgctggtg gcctcattga ttgttgcctt
    15001 taccggcagt cagggcggcg ggcagttaag tccggtgcgt ttaaccctgg cgggcgtggc
    15061 gctggcggca gtgctggaag ggctgaccag cggcatcgcc ctgcttaatc ctgacgtcta
    15121 cgatcaactg cgtttctggc aagccggttc gctggatatt cgcaatctac ataccttaaa
    15181 agtggtgctg atcccggtgc tgatcgccgg agcaaccgcg ctattactga gccgcgcgct
    15241 gaacagttta agcctcggca gcgacaccgc gacggcgctg ggcagtcgcg tggcgcgcac
    15301 acagttaatt ggtctgctgg cgattaccgt gctttgtggt agtgcgacag cggtagttgg
    15361 cccgattgcc tttattggcc tgatgatgcc gcatatggcg cgttggctgg tgggtgccga
    15421 tcatcgctgg tcgctgcccg taacgctgct tgctacccct gccctgctgc tgtttgccga
    15481 tatcatcggg cgggtgattg tgcccggtga actgcgcgtt tctgtggtca gtgcctttat
    15541 tggtgcaccg gtgctgatct tcctcgtgcg acgtaaaacg cgaggtggtg catgatttac
    15601 gtctctcgcc gattactcat cacctgtttg ctgcttattt ccgcctgtgt ggttgcaggt
    15661 atctggggat tacgcagcgg tgccgtcacg ctggaaacct cgcaggtatt cgccgcgctg
    15721 atgggcgatg cgccgcgcag tatgacgatg gtggtcaccg aatggcgttt accacgcgtg
    15781 ctgatggcgc tgttgattgg cgcggcactg ggcgtcagcg gtgcgatttt tcagtcgctg
    15841 atgcgtaacc cgctcggcag ccctgacgta atgggcttta acaccggggc gtggagcggc
    15901 gtgctggtgg cgatggtgct gtttggtcag gacctgacgg ctatcgcgct ggcagcaatg
    15961 gtgggcggca ttgtcacttc tctgttggtc tggctgctcg cctggcgtaa cggtatcgac
    16021 acctttcggt tgattattat cggcatcggc gttcgcgcca tgctggtggc ctttaatact
    16081 tggctgttgc tgaaagcgtc tttagaaacg gcgctaacag cgggtttgtg gaatgccgga
    16141 tcgctcaatg gcctgacgtg ggcaaaaact tcgccttccg cacccatcat tatattgatg
    16201 ctcattgccg ccgccttgct ggtgcggcga atgcgcttgc tggagatggg cgatgatacc
    16261 gcctgcgcgc tgggcgtcag cgtcgagcgt tcgcgtctgt taatgatgct ggttgcagtg
    16321 gtgcttaccg ctgcggcaac ggcgcttgcc gggccgattt cctttattgc tttagtcgcg
    16381 ccgcacattg cccgacgcat tagcggcacc gctcgctggg ggctgaccca ggcggcgctg
    16441 tgcggagcgc tgttactgct ggcggccgat ctctgcgccc aacaactgtt tatgccgtat
    16501 caacttccgg ttggcgtcgt taccgtcagc ctcggcggta tttaccttat cgtcttgtta
    16561 attcaggagt ctcgcaaaaa atgaccgaat cagtagcccg tttgcgcggc gaacagttaa
    16621 ccctgggata tggcaaatat accgttgcgg aaaatctgac tgtagaaatt cctgatggtc
    16681 acttcacggc aattatcgga ccaaatggct gtggtaaatc cacgttactg cgtaccttaa
    16741 gccgcctgat gacgcctgct cacgggcatg tctggctgga tggcgagcac attaaacatt
    16801 acgccagtaa agaagttgca cgccggattg gtctgttggc gcaaaatgct accacgccgg
    16861 gcgatatcac cgtgcaggag ctggtggcgc gtggacgtta tccgcatcaa ccgctgttta
    16921 cccactggcg caaagaggat gaagaagcgg taacgaaagc gatgcaggcc acgggaataa
    16981 ctcatctggc agatcaaagc gtggataccc tttctggcgg acaacgccag cgagcgtgga
    17041 tcgcgatggt gctggcccag gaaacggcaa ttatgctgct cgacgaaccc acgacctggc
    17101 tggatatcag tcatcagatt gatctactgg aactgttaag cgaactgaac cgcgagaaag
    17161 gctataccct ggcggcggtg ctgcacgatc ttaatcaggc ctgtcgttac gccagccatt
    17221 tgattgcatt gcgggaaggg aaaattgttg ctcagggagc gccgaaggag attgtcactg
    17281 ctgaactgat tgagcgcatt tatggtctgc gatgcatgat cattgacgat ccggtagccg
    17341 gaacgccgct tgtggtgccg ctcgaacgaa cggcaccttc aaccgcaaat agttaaacta
    17401 agtggtctgc catcatggca tcctgttttc tggatgccat cgcatagcgc aataacacgc
    17461 taccacaagc caccatcccg ccgatcagcg cggaaaggat cacaataatc gccttacccg
    17521 gaccgtcttt tttcactggc aatgacgggc ttaactgata tttaaacggc gtaaaattca
    17581 catcgttgac atttgctttt gttaattgct cgacaagata ctgccgatta cgtaattcac
    17641 cgttcagttc cgcaacgtca gtaaccgctt tttcaatttc cagtttgcgt tcaataccgt
    17701 ctgcgccgag agaaatagag aaatcggggt catctttaac ggcctgacca ttactgtata
    17761 cgggcttttt aattcctgcc gcgttggcaa tgtcgagtga ataattgagg cgctgaatgt
    17821 ttgcatcaag ttgatttttc gttttaatgc gatcctgagc cagtttttct ttttcaaact
    17881 gggttttgat ctccagttta ttacggacgt tttctatcga ctctttcacc accaacgcag
    17941 agatataatc gatatacccg ctcaaaacgg tctgcgcctc tttactggtt ggcgcggtaa
    18001 aacttagcgt ccaggaggta tacagtgacg gttcatcttt tttcttactg gcattgtcat
    18061 caaccgcttt cattttttcg ctcaatgcaa caatagcgcg atgcaaatcc agttcatcga
    18121 ttttcgcctc ttttaattgg tccatcacat aaggtgatga acgcaggtac tcttccagca
    18181 agctaacaga ctgaaacttc ttgataaaca ggttaaatgc accgccacgg tcgatattaa
    18241 catcgagatc aagaacgcgg agtttggtaa aggttttctc caggtcctgc cactgaatgg
    18301 cttcggcggg agtaataacg gctgagctgg tccatttttg cggcaggatg aaagagatca
    18361 gtaaacctgc gcaggcaaat gcaaaaacga ccgccatgac cgtttttttg gcccgccata
    18421 aaacttcgat tagattaagt aaatcaattt cattactact gggcgacgcc agaggatact
    18481 cggaaaaatg agcgtcactt ccctgtttaa tattcagtga tgacatgggt ataacctgaa
    18541 aatgagtcca atagggggaa atctccacgg cgctcacttt accagttggt gagaattatc
    18601 cgaagctgaa gtttgtaaat tccttccact gaatgcaaca ttcactctat ttaagagaaa
    18661 tattcatcaa acctataaat caaacaaata aaaaacccat aattattttc tgtaattatg
    18721 ggtttataga taatattaat ttacctgttt agcgttgcgc gaataatcgg cccaattttt
    18781 acaaacgccc ctggagagat aatatccaca tgcgcacaat cctgacgata gatatccagc
    18841 tcggcaatcc agggcgacca agcgcgttcg gggctcatac cttcctgaag cgtgcgttca
    18901 gcaacaaaca gcgtcgcttt accatcaaac ggtacgctat gagcagtcgt cagcagacgc
    18961 acagcatcag cgtagttgcc ttcaatggtg gtaaacaact ccgttgaagt acttccctgc
    19021 tgtgccgcca ggaaggcctc gcgttcgcgg ttaatctccg ccagcacttc cgggtccaga
    19081 ccattagcct ctttttcctg ccagttttgc gtttctggcg gccaggtatc cagcaaacca
    19141 agaaatgcca cctgttcgcc acgggcacgc agtcgcgccg caatgccctg cgccagcgta
    19201 ccgccaaggg aataccccag caggtaataa gggccgtgcg gttgttgttc aagtaacgtt
    19261 gccagatgcg cttcacagac ttcatccaga tttgccgcgg tatgcatggg gccattgggg
    19321 cgcggcgact gaatgccgat aatcgaccat tgtggatcga tataacgcga gagcacgcta
    19381 aactgccagg caaaaccgga tgccggatgg aaacaaaaca gcgtcgggcc attaccttca
    19441 cgcaacggca gaatggtttc gaatcccata cgccgggtgc tgtcttcttc agcatcaata
    19501 atcgttgcca gtttagcgac ggttgacgcg accatcactt gccccggcgt cacctggcgg
    19561 gcaacctgcc gacttaactg cgctgccagt ttcattgcca gtagcgaatg accaccaagc
    19621 gcaaagaaat cagcatcggc atcctgcacg tcacaaccca gcaacgacgc gaatgccgcg
    19681 gcaataatcg tttcactgcc cgcttttggc gcacgccccg gcgcttgtgc cttcagttca
    19741 ggcaacggta aggctttgcg atccagcttg ccgttggcgc taagaggtaa cagtggaagt
    19801 tgcagcagaa ccacgggcac catatgtggt ggcaatgttt cgcgaagctg cgcctgtaat
    19861 gcgctggtat ccaacggcag acctgattgc gacaccagat agcccaccaa ctgacgcgca
    19921 tcaccaccgg tggctgccgc ctggttaatc acacaggcgt gggtaacggc ttgttcgaca
    19981 tccggcagcg cctgcatcac gcgatcgatt tcgcccagtt caatacgctg cccgcgaatt
    20041 tttagctgat cgtcactgcg cccgaggtac tccaccgcgc cgttatccag ccagcgggca
    20101 acgtctccgg tacggtacat ccgttcacca gggacaaaag gatcggcaat aaagcggctg
    20161 gcggtcagat cgggtcgtcc aagatacccc tgcgccagtt gaatgccggt gagatagaga
    20221 tcacccgcca cacccggcgg caccggatgc atcatcgcat caagaatacg cagacccgta
    20281 ttccataccg gataaccaat cggcacactg ctgccgcgca cctgtgccag ttcctcgcca
    20341 aaagccggat accagctgac atctaccgcc gcttccgtcg ggccatatag attatgcaac
    20401 ggcacgctgg ttaactgttg ccattcgcgg cataaatcag ccggtaaggc ctcaccacta
    20461 cagaaaacct gtttcaacgt cgcgcaactc tggcgagcgg tttgcggcgt cagcgatgca
    20521 acaaatgctg ccagcatcga cggcacaaag tgcgtggtcg ttacgccata ttcggcaaag
    20581 aattgctgca tagcgagcgg gtcgcggtgc gcttccggtt cagccatcac cagtttagcc
    20641 ccggcaataa acggccagaa aaactcccac accgagacat caaaactgca cggcgttttt
    20701 tgggcaacga catcttcgcc tgtaagcaga taatgatttt gcatccacaa caggcggtta
    20761 acgatagccg tctgcccgac cattacccct ttcggcctgc cggtggagcc agaggtaaag
    20821 atgatataag ccgtgtgatg cggttgtgaa agttgcagcg gcgcactgcc ctgcggttta
    20881 agcggggcgt tatagcaaag gttggttaaa tctggaacat cggcaaagcg cggcagttga
    20941 tcgtcggtgg tgattaacag cgacggacgc gcatcttcca gcatcatttt caggcgatcg
    21001 tccggatagc cggtatccag cggtagccag gccgcgccag cttcaactat cgcatgtagc
    21061 gccagggtca aaaagaccga gcgcggtagc gccaccgcca cgctgtcgcc cggtttaacg
    21121 ccacgctcac gcagcagatt cgccagcgcc accacctgct cgcgcatttc ccgatagctg
    21181 aactggtaac gcgcatctgc cagcgccgga gcatccggtg tttttgccgc ttgttctgcc
    21241 accagcgcgc tcagcgtggt ttctggaatc tcaacctgag tggcgttgat ctgcgccagc
    21301 tgcgcatact cacctggcag cataatatcg acatcgccgc acaacagcgc cggatccgcg
    21361 gcgaactggg caatcagcat tttcaggcgt tcagcatgct ggattaacgt tggctcatcg
    21421 taacgctgtt tattggcgag gatctcaata ctcaaatcac cgtgttcatc cgggaacagg
    21481 gccagttcaa ggtcattaac cggaccggtt gccaaggtat gggtttgcgc ctgaacacca
    21541 ggaatatcca gttggtaatc aaataccttg atattgagta ccggaccaaa cagcggttca
    21601 tcacctgccg ctcgcccgct gtcacggaca atttgttcgg catcgtaacg ttgatgacga
    21661 cgcatttttt tcagttgtgc tgccagtcgg gttgccagtt ccggcagcgt ttcttgcgcc
    21721 gcaatgtgaa tacccaacgg caaaacgttg agcacgggtc cggtagccgt cagcgccgcc
    21781 gagcccagtc gacgcataaa gataaatcca gcggcgtagt ccatgcgatt gcacaatcgc
    21841 cccagccaca aagccgtcag cgcaagggct aaatcggtac gctgcacacc tgaaagttgc
    21901 gtagccaact ggcggaattc cccgtcggta aattccattt tcagacgtaa aatatctgcc
    21961 gaagcgctgc gccccggtaa aggtgccgga gaaagtgacg cgggcggcgg cagttgacga
    22021 cgctgttctg cccagaatgc cgcatcacgc tgccaggctt cgctttcgcg gtattgctgg
    22081 tactcttcca ctacatcagc gaaaggcgta aacggcgaag caggcgttgg ttcaccacgc
    22141 agccatgtgc agtaaatatt ggcgatctgg cgggtaatgg ccgggaaact gaagccatcg
    22201 accagcaaat ggtgataacg ctgataccag taccagcggt tatccgccac ctgtatcagc
    22261 tgatgaaaga ccagtggttt accgctatcg acgcgcagat cttgttgcaa atccgcctgc
    22321 attaatgcct gcgcagtacc gtgcggatca atgttggttc gcaggtcgat aatttctggc
    22381 agttcgaacg tcagcgcatc atcgacccac tgccagactt cgccgttatc ttccgtaaaa
    22441 cgcatccgca gcgtatctgc ttgcgctagt ccgtcaacca ccgcgcgggc cagtaatggc
    22501 gcatcaacct ctccggttaa ctcaacgtaa tgcgctacgc tccaggcgga gggtaaatcc
    22561 gacagttttt ctgccatcca gatgccgggc tgtgcggcga ccaaaggtaa atgctggctc
    22621 attgcgcctc ctgcaactgg gtgaaattcg ccggtgtcag agtacgccag tgggcttcaa
    22681 gccactgctg gcaggactct tgtgactgcg gctgacacac aatgtcccag cctgccggta
    22741 aggcgcattg ttgcggccac agactgaatt gcccctgcgc attgcgcaaa atgtaaaacg
    22801 ctccctgtgg atcatcgaag ggattactga atgccatatt caactcctgt catggaaaag
    22861 tggttgccag aggtcgatta gcccctgcat caagccaccg cgccaacaaa gcgcatcatg
    22921 tccgccgtca acctggcgcc agaaaatgga ttcttttatg gggtgtaatt gcgcatacag
    22981 cgcctgattg gcccgcataa tcatcggctc gcgaataccc gcttccagca caatgcgcag
    23041 accttcggcg ctaacttcac cagctttaag cttttcaagt aacacgccct cttgctgccc
    23101 gccccgatgc ggccaccagt acgatcctga ctggcttaat acacaaccaa aacgttcagg
    23161 ccagtgcagt ccggcataca gcgcggaaag tccaccaaaa ctctgcccgg caaccacggt
    23221 gcgatcggcg cgatcgctaa aaggggcaat agctttcacc aggggtaata actcttgctg
    23281 tactgcgagc cagaaatccg cattacacgg cagttcgtga gcgcggtgcg tggtgtcgat
    23341 agcgtcgatc aacacataca cggcgggagg aagttgctga cgatgggtca gcgaagtcag
    23401 cactggccag acgggcatac tttgcgccca aaattcgcca tcgagcaaaa ctgccagcgg
    23461 gcgttcttct gctgttgcat cgccggtggt aaaaatccat acacgacgtg aatttttcaa
    23521 ccgttcactt ttccagataa tttctttggc tggtatttct ggcgcttgcg gacaatccca
    23581 tcccggttgc agaggcgctt gcggcatttc gagtgcagaa acagcgtgcc ctcgcccgcc
    23641 tttccagctt tgtggattca gcggatcggc tattgcctgg ggtaatagtt ttcgccagcc
    23701 ttcacgtaat tcgaggcgat cgggggatgg tgcagaaaaa atgtcatcgc gttcggtggg
    23761 aataaagcag tagctgccgc gccagttggc attgagttgt gtcgtccact gccagacatc
    23821 agtacctgca atgcgctgca tcgactgggg ctggctgttc tgatgatgat cggtcacacc
    23881 agtgatgtag acccatacgc gctttatcgg cgagtattct tcagaacctt ggggatcacg
    23941 ccaccagaaa gtgacctcaa acatttcgtc attcagacgc tgccattccg ggccatgttt
    24001 cgactgccac cagctctcac ttcctacttt taacgccgtc acaccataac cccatgttta
    24061 ctgtgcaatt tttcattgat tgcagaaata tattgataat attattgata actatttgca
    24121 tttgcaatag cgtaatggcg cgccgtggga agcgcggaca ttaattaacc aactgcactg
    24181 cgtgtctttc aggatcaaag gttttcgcgg tagcgggatg cgtcgtgttg aggacgacca
    24241 tgcccgacag ttgcaattcg tggcaaaaat gcaggaataa aacaatgaac aagaagattc
    24301 attccctggc cttgttggtc aatctgggga tttatggggt agcgcaggca caagagccga
    24361 ccgatactcc tgtttcacat gacgatacca ttgtcgttac cgccgccgag cagaacttgc
    24421 aggcacctgg cgtttcgacc atcaccgcag atgaaatccg caaaaacccg gttgcccgcg
    24481 atgtgtcgga gatcatccgt accatgccag gcgttaacct gaccggtaac tccaccagtg
    24541 gtcagcgtgg gaataaccga cagattgata ttcgcggtat gggtccggaa aacacgctga
    24601 ttttaattga cggcaaaccg gtcagcagcc gtaactcggt gcgtcagggc tggcgtggcg
    24661 agcgcgatac ccgtggtgat acttcctggg tgccgcctga aatgattgaa cgtattgaag
    24721 ttctgcgtgg tccggctgca gcgcgttatg gcaacggcgc ggcgggtggc gtagttaaca
    24781 tcattaccaa aaaaggcagc ggtgagtggc acggttcctg ggacgcttat ttcaatgcgc
    24841 cagaacataa agaggaaggt gccactaaac gcaccaactt cagtctgacc ggcccgctgg
    24901 gcgacgaatt cagcttccgc ttgtatggca acctcgacaa aacccaggct gacgcgtggg
    24961 atatcaacca gggccatcag tccgcgcgtg ccggaacgta tgccacgacg ttaccagccg
    25021 ggcgcgaagg ggtgatcaac aaagatatta atggcgtggt gcgctgggac ttcgcgcctc
    25081 tgcaatcact cgaactggaa gcgggttaca gccgccaggg taacctgtat gcgggcaata
    25141 cccagaacac caactctgac gcttacactc gctcgaaata tggcgatgaa accaaccgcc
    25201 tgtatcgcca gaactactcg ctgacctgga acggtggctg ggataacgga gtgaccacca
    25261 gcaactgggt gcagtatgaa cacacccgta actcgcgtat tccggaaggt ctggcgggcg
    25321 gtaccgaagg gaaatttaac gaaaaagcga cacaggattt cgtcgatatc gatcttgatg
    25381 acgtgatgct gcacagcgaa gttaacctgc cgattgattt cctcgttaac caaacgctga
    25441 cgctgggtac ggagtggaat cagcaacgga tgaaggactt gagttccaat acccaggcgc
    25501 tgaccggggc gaacaccggc ggcgctattg atggtgtgag tgccaccgac cgtagcccgt
    25561 attcaaaagc agaaattttc tcgctatttg ccgaaaacaa catggagctg actgacagca
    25621 ccatcgtaac gccggggctg cgtttcgatc atcacagtat tgtcggcaat aactggagcc
    25681 cggcgctgaa catatcgcaa ggtttaggcg atgacttcac gctgaaaatg ggcattgccc
    25741 gcgcctataa agcgccgagc ctgtaccaga ctaacccgaa ctacattctc tacagtaaag
    25801 gtcagggctg ctatgccagc gcgggcggct gctatctgca aggtaacgat gacctgaaag
    25861 cagaaaccag catcaacaaa gagattggtc tggagttcaa acgcgacggc tggctggcgg
    25921 gcgtcacctg gttccgtaac gattatcgca ataagattga agcgggctat gtggctgtag
    25981 ggcaaaacgc agtcggcacc gatctctatc agtgggataa cgtgccgaaa gcagtggttg
    26041 aaggtctgga aggatcgtta aacgtaccag ttagcgaaac ggtgatgtgg accaataaca
    26101 tcacttatat gctgaagagt gaaaacaaaa ccacgggcga ccgtttgtcg atcatcccgg
    26161 agtacacgtt gaactcaacg ctgagctggc aggcgcggga agatttgtcg atgcaaacga
    26221 ccttcacctg gtacggtaag cagcagccga aaaaatacaa ctacaaaggt cagccagcgg
    26281 ttggcccgga aaccaaagaa atcagtcctt acagcattgt gggtctgagc gcgaccaggg
    26341 atgtgacgaa gaatgtcagt ctgaccggcg gcgtggacaa cctgttcgac aaacgtttgt
    26401 ggcgtgcggg taatgcccag accacgggcg atctggcagg ggccaactat atcgcgggtg
    26461 ccggggcgta tacctataac gagccgggac gtacgtggta catgagcgta aacactcact
    26521 tctgatgcta acgtcagatt gttgataaag tgcgcgtcgt tcatgccgga tgcggcgtga
    26581 acgccttatc cggcctacaa aaatcttgcc aattcaatat attgcaggac catgtaggcc
    26641 tgataagcgt agcgcatcag gcagtttagc gtttgtcacc agtctctaat atggtcgaca
    26701 tgaaaactac gcatacctcc ctcccctttg ccggacatac gctgcatttt gttgagttcg
    26761 atccggcgaa tttttgtgag caggatttac tctggctgcc gcattacgcg caactacaac
    26821 acgctggacg gaagcgcaaa gccgaacatt tagccgggcg gatcgctgca atttatgcgc
    26881 tgcgggaata tggctataaa tgtgtgcccg caatcggcga gctacgccaa ccggtgtggc
    26941 ctgcggaggt atacggcagt attagccact gcggggctac ggcattggcc gtggtttccc
    27001 gtcaaccgat tggcgttgat atcgaagaaa ttttttctgc gcaaacagcg accgaattga
    27061 cagacaacat tattacacca gcagaacacg agcgactcgc agactgcggt ttagcctttt
    27121 ctctggcgct gacactggca ttttccgcca aagagagcgc atttaaggca agtgagatcc
    27181 aaactgatgc aggttttctg gactatcaga taattagctg gaataaacag caggtcatca
    27241 ttcatcgtga gaatgagatg tttgctgtgc actggcagat aaaagaaaaa atagtcataa
    27301 ctctgtgcca acacgattaa ttgacaacat ctggtacgat tcgcccgcag ccatcactga
    27361 ccgcgggcga aagtgtaaag caggtgcctt accatcctga cctgacaacc ggatatgcgg
    27421 ggattgctcc ccgcacaacg gctacttctt cggttcgtaa gcgagaacag ccttaaactc
    27481 aatattacgt tcctttacgg tgaactcaca cagcgaatcc ccgaccagaa gcgtaaatcc
    27541 cagcaccgtt aaacacagca ctatgactgc cgcaagggca tatttcgtca gcatgtttat
    27601 attgcctcca tgcgaagaag ggactaccat ccaaattgtt caggtttggg taggtagccc
    27661 cagggttgat agaaatatcg tcctggggct tcatctttcc agacctcaga gtaaacctga
    27721 aaccggaaag cttcaggcac ccgcagttat cttacccttg cttagcaaaa tagctgttta
    27781 tttatacagt aagtcctgtt tcggacttct cactccccat ttgatcaact gaagctgaac
    27841 ccgttttttt ctgcatgcct caatcgtcaa aattcttcgc cacagtcagc actgccataa
    27901 cttgtcacat tgcaaaagta gccaaattgc ttctgtcata atcagttcac catcaccaaa
    27961 aaaaagaaac attccagaac ttccgcatct cgcccagccg ctcccacttc tatactgaat
    28021 agaaaacgcc aacataagag aaacctatgc cattacccga ttttcatgtt tctgaacctt
    28081 ttacactcgg tattgaactg gaaatgctgg tggttaatcc gccgggctat gacttaagcc
    28141 aggactcttc aatactgatt gacgcggtta aaaataagat cacggccgga gaggtaaagc
    28201 acgatatcac cgaaagtatg ctggagctgg cgacggatgt ttgccgtgat atcaaccagg
    28261 ctgccgggca attttcagcg atgcagaaag tcgtattgca ggcagccgca gaccatcatc
    28321 tggaaatttg cggcggtggc acacacccgt ttcagaaatg gcagcgacag gaggtatgcg
    28381 ataacgagcg ctatcaacga acgctggaaa actttggtta tctcattcaa caggtgaccg
    28441 tttttggtca gcatgttcat gttggctgcg ccagtggcga tgacgccatt tatttgctgc
    28501 acggcctgtc acggtttgtg ccgcacttca tcgccctttc cgccgcgtcg ccatatatgc
    28561 aaggaacgga tacgcgtttt gcctcctcac gaccgaatat tttttccgcc tttcctgata
    28621 atggcccgat gccgtgggtc agtaactggc aacaatttga agccctgttt cgctgtctga
    28681 gttacaccac gatgatcgac agcattaaag atctgcactg ggatattcgc cccagtcctc
    28741 attttggcac ggtggaggtt cgggtgatgg ataccccgtt aacccttagc cacgcggtaa
    28801 atatggcggg attaattcag gctaccgccc actggttact gacagaacgc ccgttcaaac
    28861 atcaggagaa agattacctg ctgtataaat tcaaccgttt ccaggcctgt cgctatgggc
    28921 tggaaggcgt cattaccgat ccgcacactg gcgatcgtcg accgctaacg gaagacacct
    28981 tgcgattgct ggaaaaaatc gccccttccg cacataaaat tggtgcatcg agcgcgattg
    29041 aggccctgca tcgccaggtc gtcagcggtc tgaatgaagc gcagctgatg cgcgatttcg
    29101 tcgccgatgg tggctcgctg attgggctgg tgaaaaagca ttgtgagatc tgggccggtg
    29161 actaagtagg gaattgcctt acagaagaat atccctgaca atggtgtttt aacttacatt
    29221 taacaatgtt atgaaacacc ctttagaaac cttgactacc gcagcaggca ttttgctgat
    29281 ggctttcctc tcttgcctgc tgctgcccgc ccccgcactg gggctgacgc tggcacaaaa
    29341 actggtgacc atgttccatc tgatggatct tagccagctt tacactttat tgttttgtct
    29401 gtggttttta gtgctgggcg ctattgagta ttttgttctg cgctttatct ggcgacgctg
    29461 gttctcgctg gcggattaaa cgtcgattat cgccgtactt cgcggcatac tttgcttatt
    29521 ctccttcgcc taaaggaacg tttatggata agcaatcact gcacgaaacg gcgaaacgcc
    29581 tggcccttga gttacccttt gtcgagcttt gctggccttt tggcccggag ttcgatgttt
    29641 ttaaaattgg cggcaaaatt tttatgctgt cgtcggagct gcgcggcgtc ccctttatca
    29701 atctgaagtc cgatccacaa aaatccctgt taaatcagca gatatacccg agcattaagc
    29761 cagggtatca catgaataaa aagcactgga tttcggtgta tcccggcgag gaaatctccg
    29821 aagcgttact tcgcgatctg atcaacgatt cgtggaatct ggtggttgat ggtctggcta
    29881 aacgcgatca aaaaagagtg cgtccaggct aaagcggaaa tctatagcgc atttttctcg
    29941 cttaccattt ctcgttgaac cttgtaatct gctggcacgc aaaattactt tcacatggag
    30001 tctttatgga tatcatttct gtcgccttaa agcgtcattc cactaaggca tttgatgcca
    30061 gtaaaaaact taccccggaa caggccgagc agatcaaaac tctcctgcaa tacagcccat
    30121 ccagcaccaa ctcccagccg tggcatttta ttgttgccag cacggaagaa ggtaaagcgc
    30181 gtgttgccaa atccgctgcc ggtaattatg tgttcaacga acgtaaaata cttgatgcct
    30241 cgcacgtcgt ggtgttctgt gcaaaaaccg cgatggacga tgcctggctg aagctggttg
    30301 ttgaccagga agatgctgat ggccgctttg ccacgccgga agcgaaagcc gcgaacgata
    30361 aaggtcgcaa gttcttcgct gatatgcacc gtaaagatct gcatgatgat gcagagtgga
    30421 tggcaaaaca ggtttatctc aacgtcggta acttcctgct cggcgtggcg gctctgggtc
    30481 tggacgcggt acccatcgaa ggttttgacg ccgccatcct cgatgcagaa tttggtctga
    30541 aagagaaagg ctacaccagt ctggtggttg ttccggtagg tcatcacagc gttgaagatt
    30601 ttaacgctac gctgcgaaat ctcgtctgcc gcaaaacatc accttaaccg aagtgtaatt
    30661 ctctcttgcc gggcatctgc ccggctattt cccctcagat tctcctgatt tgcataaccc
    30721 tgtttcagcc gtcatcatag gctgctgttg tataaaggag acgttatgca ggatttaata
    30781 tcccaggttg gggatttagc gggtattgag atcgatcaca ccacctcgat ggtgatgatt
    30841 ttcggtatta tttttctgac cgccgtcgtg gtgcatatta ttttgcattg ggtggtactg
    30901 cggaccttcg aaaaacgtgc catcgccagt tcacggcttt ggttgcaaat cattacccag
    30961 aataaactct tccaccgttt agcttttacc ctgcagggga ttatcgtcaa tattcaggcg
    31021 gtattctggc tgcaaaaagg caccgaagcg gcagatattc tgactacctg cgcgcagttg
    31081 tggatcatga tgtatgcgct gctttcagtc ttctcgttgc tggatgttat tttgaatctg
    31141 gcgcagaaat tcccggcggc atctcagtta ccgctgaaag ggatatttca ggggattaaa
    31201 ctgatcggcg caatcctggt cggcattttg atgatctcgc tgctgattgg tcagtcgcca
    31261 gcgattctga tcagcggtct tggtgcaatg gctgccgtgc tgatgttggt atttaaagat
    31321 ccgattcttg gtctggtggc aggtattcag ctttccgcga acgatatgct gaaactgggc
    31381 gactggctgg agatgccgaa atacggcgcg gatggcacgg tgatcgatat tgggttaacc
    31441 accgtcaaag tgcgtaactg ggacaatacc attaccacta ttcccacctg gtctctggtt
    31501 tctgactcct ttaaaaactg gagcgggatg tcagcatctg gcgggcgacg tattaagcgc
    31561 agtatcagta ttgatgtcac cagtattcgt tttcttgatg aagacgaaat gcaacgtctg
    31621 aataaagcgc acttgttaaa accttattta accagccgcc atcaggaaat taatgagtgg
    31681 aatcgccagc aaggttctac ggagtcgata ttaaacctgc gccgaatgac caatattgga
    31741 acctttcgtg cctatctgaa cgaatatctg cgtaaccatc cgcggattcg taaagatatg
    31801 accttaatgg tacgccaact ggctccaggt gataacggtt taccgctcga gatctacgcg
    31861 tttaccaaca ccgtggtgtg gctggaatat gaaagcattc aggctgatat attcgatcac
    31921 atatttgcga ttgtcgaaga gtttggtctg cgacttcatc agtcgccagc cggcaatgat
    31981 attcgctctc tggcgggtgc atttaagcag taattaaaaa aaccgctctc atcgaatgga
    32041 tgagagcgga catcgttttg tcggatgtag cgtgaatgcc ttatttccga cgcagcgttt
    32101 taaatgccac aaacaggaat acaatccaca ccggcagcag gatcgctgac aagcgcatat
    32161 catccatcgt gcacatcagc agcaaaatca tgccgaggaa ggcaatgcaa agatagttgc
    32221 cgaacggata aagcagcgcc ttaaactgtg tttcacgtcc ctgacgtcgc atcgccgcgc
    32281 gaaaacgcag atgcgccagg cagatcataa tccagttcaa cagcagcgtt gctaccacca
    32341 gcgccatcag cagaccaaac gctttttgcg gcagcagata gttgattaac accaccagcg
    32401 aagtgatcgc tccggaaagc atcagcgagt taatcggcac gccgcgatgg ctgacgcgag
    32461 tcaaaaactt cggcgcatta ccctgcacag aaaggccaaa cagcatgcgg ctgttagagt
    32521 aaaccccgct gttatacact gacagcgatg ctaccagaat gacgaagttc agcgcagaag
    32581 ctacctcgtt gctgtcgaga ttatggaaaa tcatcacaaa cgggctactg ttggatttca
    32641 cttccaccca cggatagagc gccagtaaaa ccaccagtga acagatgtaa aacagcagga
    32701 tgcgatacac cacctgattt accgcttttg gaatgctttt ttccggatcg cgcgcttcag
    32761 cggcagtaat cccaatcagc tccagaccgc cgaaggagaa cataattacc gccagcgaca
    32821 aaatcagccc attccagccg gtggcgaaga aaccaccgta gcgccagagg ttgtcgatac
    32881 tggctttctc gccgccgtga ccagaaaaca gcagccacag gccaaagccg atcataccga
    32941 tgattgccag cactttaatc aacgcaaacc agaactcggt ttcgccatat aagcgcacgt
    33001 tcaccaggtt aacggcgttg atgataataa agaaggcggc agcccaaatc cacgttggaa
    33061 catccgggaa ccagtactgc atatagatgc ccgcagcggt cagctctgcc attcccacca
    33121 gcacgaacat tacccagtag ttccagccag agaggaagcc cgcaaacggt ccccagtatt
    33181 tataggcaaa gtgggcaaat gaaccggata ccggctcttc gaccaccatc tcgccgagct
    33241 ggcgcataat caggaaagcg atgatcccgg cgacgccgta gcccagcaat acagccggac
    33301 ccgccatctg aatcgccggg ccaatgccaa gaaacagacc agtaccaatt gcgccaccca
    33361 gcgcaatcag ttgaatatga cggttatgta atccgcgatg aagcgtcggc tcttgatttg
    33421 acgcagtatc ttccgatacg gttgacgcgt ttttcacgcc tttcccctgt gtgtcttttt
    33481 tgttgagggg caccttttaa catttagtgc ccatcgtcgc aagacacaat ccacacggtt
    33541 aaacggggta tcctgctttt atttccgtac ccgatgtcgg tgcagccaca tcagcttata
    33601 cgccgccggg ataataaaca gcgacagcaa aggcgcggtg atcatgccgc caatcatcgg
    33661 tgcggcaatc cggctcatca cctctgaacc agcccccgtt ccccacagaa tcggcagcag
    33721 accggcgata atcaccgcca ccgtcatcgc tttcgggcgc acgcgcagta ccgcgccgtg
    33781 atacaacgcc tcatccagct tctgctcact gaatgtttgc ggattattca acgacggctc
    33841 ggcctctatg gcgtgacgta aatacatcag catcaccacg ccaaattcgg cggcgccccc
    33901 ggcaagggca ataaaaccgg tgcccgttgc cacagaaaga tgaaagccca tccaccacag
    33961 caaccagatg ccgcccacca gcgcaaacgg tacgctgctg ataatcagca acgcttcgcc
    34021 cacccgacgg aatgccagat acaacagcac gaagatgatc atcagtgtca tcggcaccat
    34081 gagtttcagc ttatggttgg cgcgctccag tagctcgaac tgcccggaga atgccacgct
    34141 ggtgcccggt tttaactgca ctttttcaac tatcgctttt tgcaaatcgt gaaccaccga
    34201 caccatgtca cgatcgcggg catcgatata aatccagctc gtcgggcgcg cattctcggt
    34261 tttcagcatc gacggtccgg tagagacttt aacgtcggcc acgtctgcca gggtgatttg
    34321 ctgcttcatc ggcgtcagga tcggtagctg gcgcagtgcc tgcgggctat cgcgccagct
    34381 ttgcggataa cgcagattaa tgggataacg ggcaatccct tccaccgttt cgccaaccat
    34441 cgccccgccc accgcagaag tcacaaacaa ctgcacatcc gccaccgtca taccgtaacg
    34501 cgcggctttt tcacggttaa tctcaacgtt gatatagcgc ccaccttcca gacgctcggc
    34561 aagcgcagaa gccacgcctg gcaccgttcg cgctacttct tcaatttgct cagccatcgc
    34621 gtcgatatcc gccagcacag tgccggaaac tttaatgccg atggggcttt taatgccggt
    34681 tgagagcata tcgatacggt tacgaattgg cggcacccac agattcgcca gccccggcag
    34741 ccgcacggtg ttatccagtt cctcaatgat tttgtccatc gtcatgccag gccgccactg
    34801 atcctgcggc ttaagctgga tggtcgtttc aaccatctcg agcggagcag aatcggtggc
    34861 ggtttccgct ttcccggttt tgccaaatac ccgcgccact tcaggtacgc tcataattag
    34921 cttgtcgttt ttttgcagca tactcgccgc ctctgctgcg gaaatccccg gcagcgtcga
    34981 tggcatatac aacaagtcgc cttcattgat ctgcggtaaa aattccccgc caactttatt
    35041 gagcggccag agaaccgtca gcaccgaaag cgctgccacc aacagcgtgg ttttcggcca
    35101 gtgcagtact ttcagcaata gaggatgata aacacgaatc aaaaagcgat tgagcgggtt
    35161 actgctttcc ggcggaattt tgccacggat ccagtaaccc atcaggatcg ggatcactac
    35221 gatcgccagc agcgccgcac ccgccatcgc atacgttttg gtgaacgcca gcgggccaaa
    35281 cagacgccct tcttgccctt ccagggtgaa gattgggata aacgacagcg tgataatcag
    35341 cagactgata aacagcgccg gccccacttc aacagatgca tcggtaatca cctgccagcg
    35401 cgttttatta tccagcgtgg cgtcaggatg ctggtgctgc cactcttcca gccgtttatg
    35461 cgcattctcg atcatgacga tagcagcatc gaccatcgcc ccgacggcaa tcgcaatgcc
    35521 acccaacgac ataatattgg cattcagtcc ctggaagggc atgacaataa aagcaataca
    35581 caaccccagc ggcaacgaaa taatcgccac cagcgccgag cgcacatgcc agagaaacag
    35641 cgcacagact accgccacca caataaactc ttccagcaac ttgccgctga ggttgtcgat
    35701 ggcgcggtca atgagctggc tgcgatcgta tgttgtaact atctccacgc cttccggcag
    35761 actacttttc agcgtttcca gtttgtcctt cacggcggcg atcacttctc gggcgttttt
    35821 gccggagcgc aggatcacca ccccgcccgc cacttcgcct tcgccgttca gttcggcaat
    35881 gccccggcgc atctccgggc caacctggac cttcgcaaca tcgcgcagat aaacgggcac
    35941 gccattttca ctggctttta aaacgatgtg attaaagtcg tcgagcgttt gcagatagcc
    36001 gctggcgcgc accatatatt ccgcttccgc cagttcgatc gacgaaccgc ccgcttcctg
    36061 gtttgaagca tccagcgcgc tttttacttc ggcgagactg atgccatact gcgccaggcg
    36121 ctggggatcg ataaccacct gatactcttt caccacaccg cccaccgacg ccacttccgc
    36181 aacgtcaggg atggttttca gctcatattt gagaaaccag tcctgtaatg agcgtaaatc
    36241 ggccagatcg tgcttaccgc tgcgatccac cagtgcatat tcatagatcc agccaacacc
    36301 cgtggcatct ggccccagct cggcgctgac tcccgcaggc agcttaccct gtacctggtt
    36361 gaggtactcc agcacccgcg aacgcgccca gtacggatcg gtaccatctt cgaaaatgac
    36421 atacacataa gagtcgccaa actgcgagaa accacgcaca gtcttcgcgc caggcaccga
    36481 caacatggtg gtggttagcg gataagtcac ctgattttca acgatttgcg gtgcctgccc
    36541 gggatagctg gttttaataa tcacctgcac atcggagaga tccggcagcg catccactgg
    36601 cgtattaatg atggtccagg tgccccagat gctaagaaac aacgcgccca tcagcaccaa
    36661 aaaacggttc gccaccgagc gacgaataat ccattcaatc attggttatt ccctcaatgc
    36721 gcatgggtag cactttcaga gcgcatccgc tccagtgcgc cagaaatatt ggcttcggaa
    36781 tcaatcagga aaaggccgct ggaaaccacc ttttcacctt ccgccagacc agagcgtaat
    36841 gcggtgacac cttgcgatgc ctggaaaacg gcaacgcgtt tcggtacaaa gcgcccgtcg
    36901 gcatcaatgg taatcacccg ctgttcgcta ccggtatcaa tcagcccttg tgacggaatg
    36961 agcagcatcg gttcgctggc ggtattgagt tgcaaccagg cgttcatgcc cggttttagc
    37021 gcctcgtcgg cgttgtcgac ttccagacgc agctgcagcg tgcgggtcgc ggcatccacg
    37081 ccgggtagca gcgtccattt gcggatggtg agtgttttat ccggtcgtgc cggaacggtg
    37141 agcgtaaact gcgaggcatc tttcaccagc caagcgatag actccgggat cgcagcagtg
    37201 acccacaccg ggtccatacc ctgaattttc gctaccacgt tatctttggc gatattcatt
    37261 cccgcgcgca gatcaaacgc ggtgatcacg ccatcaatgg gcgctttgag cgtaaagcga
    37321 gtctggattt tttgcgtggc gatcaggcgg cgaatatccg cctccggcat tcccgccagt
    37381 cgcagccgct caagaatgcc ttcagtctgg gtcgccgtac cgccggtttc gcgcagcagt
    37441 aaatactcac tctgcgcttc cacccagtca ggaatggtca ggtcgagaag cggtgtgccc
    37501 ttttgtactt tatcgcccac ggtaagcgga tacaccttgt cgataaaccc ggcagcgcga
    37561 gcctgcacaa tggcatactg atactcgttg taactgacat tcgccgggaa actctgggca
    37621 aaagtcagcg gtccgcgcgt gacggtagcc gttttcaccc ccagattctg cgtctgagtc
    37681 gggtcaatgc gcacaccaga cgcagaactc tcttcatcgg catatttcgg caccagatcc
    37741 atatccataa acggcgattt acctggttta tcgaaccgcg tattggggta catcgggtcg
    37801 taccagaata agattttacg t
//
LOCUS       DAJVUJ010000017        37319 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.017, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000017 DAJVUJ010000000
VERSION     DAJVUJ010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37319
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.017"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(<1..808)
                     /locus_tag="OQQ48_000838"
     CDS             complement(<1..808)
                     /locus_tag="OQQ48_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001019361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HCS1951693.1"
                     /translation="MTIEKSRFSSGAAPQPNVNTGVINGNDFAAIVPVIAGRIGGREA
                     NIVSAKALHKALGVGRDFTNWIKGRIDQYGFIAGTDFIRVENLSTPKRASAKTRQQIE
                     HDYLLSLDMAKEVAMVERNEQGRAIRRYFIQCEEELQRSVPEIAARYRRQLKARLSAA
                     NNFKPMCDALNMARAEMGKTTQQRHYSNESNMIARIVLGGMTAKQWAQANGITGEPRD
                     SMNAAQLEHLAYLESTNITLIDMGMEYEQRKGELTRLSQRWLAKRLEVNHA"
     gene            complement(822..1253)
                     /locus_tag="OQQ48_000839"
     CDS             complement(822..1253)
                     /locus_tag="OQQ48_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001437021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1951694.1"
                     /translation="MEKKNRPLQAANSDIRVSDVTPLTKSLQAPKRTPKKHRARVYML
                     RTGIEGWTENDILRYCRLSSGRNYASELERRLDIQLERIDEKNPDGIGSHLRYRFTGR
                     GDVLKVIRLVNHNADAGGYQGLSQPEIADILNLYPDNLTAA"
     gene            complement(1253..1456)
                     /locus_tag="OQQ48_000840"
     CDS             complement(1253..1456)
                     /locus_tag="OQQ48_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HCS1951695.1"
                     /translation="MNIDTIPTKGYIRRFRLAELLGVSVSTIDRKVRNGSLPRPVKLG
                     EKITAFDAVEIHNWLAERRGKVA"
     gene            complement(1569..2417)
                     /locus_tag="OQQ48_000841"
     CDS             complement(1569..2417)
                     /locus_tag="OQQ48_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1951696.1"
                     /translation="MKKLPPAPFFRLNRAAELLEIDIDDLLIMAAAESIYLSAYLCGY
                     DGTLHSIASLYRNCSLFPDVKEDCLLLADIITEVEDEKSFKAGVVNHIDCHVLGLWHV
                     NASYILDIAGLDSIDTPARIFSPAHRKRELDVSANYYFFLDYTKHAFHEISVEDLYIT
                     RPWVEYIGECMDKNQPIYSPETRINLQALYEDVKNVEHGNRERHRQKREICLLALIYV
                     KRHYPEECKGKNGKETNEAWANATINHWALLGGGYDEPSIDYLKKLIADADRTPEERT
                     TAGKKR"
     gene            complement(2513..3772)
                     /locus_tag="OQQ48_000842"
     CDS             complement(2513..3772)
                     /locus_tag="OQQ48_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCS1951697.1"
                     /translation="MPKLTDMQIRAWIKSGERFEGRADGNGLYLRYREADKTPTWRFR
                     YKLAGKSRAMLIGSYSELSLSKARETAKELSARVALGYDVAGEKQKRKTEALAKMEAE
                     KNAMRVSELAAEYFERQILPRWKHPDILRRRIDKDINPCIGSMKVEDVKPRHIDDMLK
                     GIVDRGAPTIATDVLRWTRRIFDYGIKRHALEINPCSAFEVADAGGKEAARDRWLTRD
                     ELIQLFKAMRTAKGFSRQNEITFKLLLALCVRKMELCAARWEEFDLDGAVWHLPEERS
                     KNGDPIDIPLPSPAVEWLRELHTFSCNSAWVLPARKMQNRMIPHIQESTLPVALAKVR
                     AEMPDVPNFTIHDFRRTARTHLAALGVDPVVAERCLNHRIKGVEGIYNRHQYFDERKA
                     ALAQWADLLVALESGKDYNVTPLRRAN"
     gene            complement(3963..4826)
                     /locus_tag="OQQ48_000843"
     CDS             complement(3963..4826)
                     /locus_tag="OQQ48_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HCS1951698.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDISAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            4953..5669
                     /gene="rph"
                     /locus_tag="OQQ48_000844"
     CDS             4953..5669
                     /gene="rph"
                     /locus_tag="OQQ48_000844"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HCS1951699.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            5735..6376
                     /gene="pyrE"
                     /locus_tag="OQQ48_000845"
     CDS             5735..6376
                     /gene="pyrE"
                     /locus_tag="OQQ48_000845"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HCS1951700.1"
                     /translation="MKPYQRQFIEFALGKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGLEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(6413..7009)
                     /gene="slmA"
                     /locus_tag="OQQ48_000846"
     CDS             complement(6413..7009)
                     /gene="slmA"
                     /locus_tag="OQQ48_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HCS1951701.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(7116..7574)
                     /gene="dut"
                     /locus_tag="OQQ48_000847"
     CDS             complement(7116..7574)
                     /gene="dut"
                     /locus_tag="OQQ48_000847"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HCS1951702.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            complement(7552..8772)
                     /gene="coaBC"
                     /locus_tag="OQQ48_000848"
     CDS             complement(7552..8772)
                     /gene="coaBC"
                     /locus_tag="OQQ48_000848"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HCS1951703.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEGLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSENNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            8944..9612
                     /gene="radC"
                     /locus_tag="OQQ48_000849"
     CDS             8944..9612
                     /gene="radC"
                     /locus_tag="OQQ48_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HCS1951704.1"
                     /translation="MKNNSQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            9829..10065
                     /gene="rpmB"
                     /locus_tag="OQQ48_000850"
     CDS             9829..10065
                     /gene="rpmB"
                     /locus_tag="OQQ48_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCS1951705.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARSEKY"
     gene            10086..10253
                     /gene="rpmG"
                     /locus_tag="OQQ48_000851"
     CDS             10086..10253
                     /gene="rpmG"
                     /locus_tag="OQQ48_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCS1951706.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            10351..11160
                     /gene="mutM"
                     /locus_tag="OQQ48_000852"
     CDS             10351..11160
                     /gene="mutM"
                     /locus_tag="OQQ48_000852"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HCS1951707.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(11199..11678)
                     /gene="coaD"
                     /locus_tag="OQQ48_000853"
     CDS             complement(11199..11678)
                     /gene="coaD"
                     /locus_tag="OQQ48_000853"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCS1951708.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(11686..12963)
                     /gene="waaA"
                     /locus_tag="OQQ48_000854"
     CDS             complement(11686..12963)
                     /gene="waaA"
                     /locus_tag="OQQ48_000854"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HCS1951709.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQVLLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            13376..14434
                     /gene="rfaQ"
                     /locus_tag="OQQ48_000855"
     CDS             13376..14434
                     /gene="rfaQ"
                     /locus_tag="OQQ48_000855"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HCS1951710.1"
                     /translation="MDKLFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLRVDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            14431..15555
                     /locus_tag="OQQ48_000856"
     CDS             14431..15555
                     /locus_tag="OQQ48_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCS1951711.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     YPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYHLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            15548..16345
                     /gene="rfaP"
                     /locus_tag="OQQ48_000857"
     CDS             15548..16345
                     /gene="rfaP"
                     /locus_tag="OQQ48_000857"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HCS1951712.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWSAIHRLRDVGVDTMYGVAFGEKGI
                     NPLSRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            16361..17377
                     /gene="waaO"
                     /locus_tag="OQQ48_000858"
                     /gene_synonym="rfaI"
     CDS             16361..17377
                     /gene="waaO"
                     /locus_tag="OQQ48_000858"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HCS1951713.1"
                     /translation="MSAHYFNPQEMINKTIIFDERPAASVASSFHVAYGIDKNFLFGC
                     GVSITSVLLHNNDVSFVFHVFIDDIPEADIQRLAQLAKSYRTCIQIHLVNCERLKALP
                     TTKNWSIAMYFRFVIADYFIDQQDKILYLDADIACQGNLKPLITMDLANNVAAVVTER
                     DANWWSLRGQSLQCNELEKGYFNSGVLLINTLAWAQESVSAKAMSMLADKAIVSRFTY
                     MDQDILNLILLGKVKFIDAKYNTQFSLNYELKKSFVCPINDETVLIHYVGPTKPWHYW
                     AGYPSAQPFIKAKEASPWKNEPLMRPVNSNYARYCAKHNFKQNKPINGIMNYIYYFYL
                     KIIK"
     gene            17394..18389
                     /locus_tag="OQQ48_000859"
     CDS             17394..18389
                     /locus_tag="OQQ48_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide 1,2-glucosyltransferase"
                     /protein_id="HCS1951714.1"
                     /translation="MNEFIKERFSYLADNKKENAPELNVSYGIDKNFLYGAGVSISSV
                     LINNSDINFVFHVFTDYVDDDYLKSFNETAKQFNTSIIVYLIDPKYFADLPTSQFWSY
                     ATYFRVLSFEYLSESISTLLYLDADVVCKGSLKPLTEIIFKDEFAAVIPDNDSTQAAC
                     AKRLNIPEMNGRYFNAGVIYVNLKKWHEANLTPYLLKLLRGETKYGSLKYLDQDALNI
                     AFNMNNIYLAKDFDTIYTLKNELYDRSHRKYQQTITDKTVLIHYTGITKPWHSWAGYP
                     SASYFNIAREQSPWKKYPLKEARTVAEMQKQYKHLFAHGEYIKGITSLIKYKLKK"
     gene            18399..19091
                     /gene="rfaY"
                     /locus_tag="OQQ48_000860"
     CDS             18399..19091
                     /gene="rfaY"
                     /locus_tag="OQQ48_000860"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HCS1951715.1"
                     /translation="MITSIRYRGFSFYYKDNDNKYKEIFDEILAYNFKTVKVLCNIDD
                     TKVSLIDTKYGRYVFKVFAPKTKRNERFLKSFVKGDYYQNLIVETDRVRSAGLTFPND
                     FYFLAERKIFNYASVFIMLIEYVEGVELNDMPIIPENVKAEIKASMEKLHALNMLSGD
                     PHRGNFIVSKDGVRIIDLSGKSCTAERKARDRLAMERHLGIANEIKDYGYYSVIYRTK
                     LRKFIKKLKGKA"
     gene            19117..20142
                     /locus_tag="OQQ48_000861"
     CDS             19117..20142
                     /locus_tag="OQQ48_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactose--(galactosyl) LPS
                     alpha1,2-galactosyltransferase"
                     /protein_id="HCS1951716.1"
                     /translation="MDLLAESITEVAVSGEIANTDRVLNIAYGIDRNFLFGAAVSMQS
                     VVMHNPDLAVKFHLFTDYIDEDYLQRVNAFTSKNANVEVIIYKVSNAFIDIFPSLKQW
                     SYATFFRLVAFQYLSETIENLLYIDADVICKGSLAGLLDINFDEDKFAAVIKDVPFMQ
                     EKPAKRLAIEGLPGNYFNAGVVYLQLEAWAKNDFMNKAIAMLASDPQHTKYKCLDQDI
                     LNILFFGHCNFISGDYDCFYGIDYELKNKSDEDYKKTITDDTKLIHYVGVTKPWNDWT
                     NYPCQKYFNEAYQASCWNDVAFIPATNEKQYQVKYQHAKKNGDTFNAFIYFIKFKLNK
                     YKRKLFG"
     gene            20227..21210
                     /locus_tag="OQQ48_000862"
     CDS             20227..21210
                     /locus_tag="OQQ48_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCS1951717.1"
                     /translation="MSNDYPLVSIIIPTYNSSDYITETLTKLEKQTYPNFEIVIVNDG
                     SKDNTSNVLREYGLTHSRLIIVNKENGGVSSARNTGIRKAQGQFICFMDDDDEIDPNY
                     LLKMYTRQHETGGDAIYCGLYGHYIKDGVTYSPINTEFNEGSLLFDFFYKKVRFHIGC
                     LFIRKQLLEENNLFFDEDLRLGEDLDFIYRLLITCDMYAVPYYMYKHNYRENSLMNSC
                     RTITHYRHESFAHERIYSSVMQLYKGNRKEEIHTLLSQNRAYHKTRYLWNVLLNGDFK
                     LLNQLVESNEKELRYCNLPGKRDKRRAKILASKNYILWRMVRLVNRKKNKR"
     gene            21256..22509
                     /locus_tag="OQQ48_000863"
     CDS             21256..22509
                     /locus_tag="OQQ48_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCS1951718.1"
                     /translation="MSFCWNEINSGIKSLILILCIFSLMTLSLWDDVATKFLHAAGII
                     SALYFLATPKKTITNNPTLLIFISLCLLGIVNIIWYSHYKVSGSVYTNAYRGPMETGK
                     IALCSAFIFLVLFAKNEMRTKIKFGKLILFASLATQLLFFAHAMWQHFYLNVDRVALS
                     ASHATTAGYIILFPSLLASILILKSDFRHKTTLYTINFMLSLCAVIVTETRAAILVFP
                     FFALILIVMDSYINKRINYKLYCFIAIALLAGVFSFKDTLLMRMNDLNNDLVNYSHDN
                     TRTSVGARLAMYEVGLKTYSPIGQSLEKRAEKIHELEEKEPRLSGALPFVDSHLHNDL
                     IDTLSTRGIPGVVLTILAFSAILIYALRTAKEPYILILLFSLLVVGLSDVILFSKPVP
                     TAVFVTIILLCAYFKAQSDQYLLDK"
     gene            complement(22579..23559)
                     /gene="rfaC"
                     /locus_tag="OQQ48_000864"
     CDS             complement(22579..23559)
                     /gene="rfaC"
                     /locus_tag="OQQ48_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HCS1951719.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFDYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQMVCRAPGNELSQLTANAVKRFIEENAENAAMI"
     gene            complement(23563..24609)
                     /gene="rfaF"
                     /locus_tag="OQQ48_000865"
     CDS             complement(23563..24609)
                     /gene="rfaF"
                     /locus_tag="OQQ48_000865"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HCS1951720.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNTLLLQEEA"
     gene            complement(24619..25551)
                     /gene="rfaD"
                     /locus_tag="OQQ48_000866"
     CDS             complement(24619..25551)
                     /gene="rfaD"
                     /locus_tag="OQQ48_000866"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HCS1951721.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            25765..26961
                     /gene="kbl"
                     /locus_tag="OQQ48_000867"
     CDS             25765..26961
                     /gene="kbl"
                     /locus_tag="OQQ48_000867"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCS1951722.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            26971..27996
                     /gene="tdh"
                     /locus_tag="OQQ48_000868"
     CDS             26971..27996
                     /gene="tdh"
                     /locus_tag="OQQ48_000868"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HCS1951723.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            28235..29269
                     /gene="waaH"
                     /locus_tag="OQQ48_000869"
                     /pseudo
     CDS             28235..29269
                     /gene="waaH"
                     /locus_tag="OQQ48_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
     gene            complement(29256..30215)
                     /locus_tag="OQQ48_000870"
     CDS             complement(29256..30215)
                     /locus_tag="OQQ48_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HCS1951724.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPLTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(30219..31502)
                     /gene="envC"
                     /locus_tag="OQQ48_000871"
     CDS             complement(30219..31502)
                     /gene="envC"
                     /locus_tag="OQQ48_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HCS1951725.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQCGQRLQAYF
                     GYLNQARQETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            complement(31512..33056)
                     /gene="gpmM"
                     /locus_tag="OQQ48_000872"
                     /gene_synonym="pgmI"
     CDS             complement(31512..33056)
                     /gene="gpmM"
                     /locus_tag="OQQ48_000872"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HCS1951726.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            33301..33732
                     /locus_tag="OQQ48_000873"
     CDS             33301..33732
                     /locus_tag="OQQ48_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCS1951727.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            33874..34125
                     /gene="grxC"
                     /locus_tag="OQQ48_000874"
     CDS             33874..34125
                     /gene="grxC"
                     /locus_tag="OQQ48_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HCS1951728.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            34188..34655
                     /gene="secB"
                     /locus_tag="OQQ48_000875"
     CDS             34188..34655
                     /gene="secB"
                     /locus_tag="OQQ48_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HCS1951729.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            34655..35674
                     /gene="gpsA"
                     /locus_tag="OQQ48_000876"
     CDS             34655..35674
                     /gene="gpsA"
                     /locus_tag="OQQ48_000876"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCS1951730.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMCPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGAEPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            35754..36575
                     /gene="cysE"
                     /locus_tag="OQQ48_000877"
     CDS             35754..36575
                     /gene="cysE"
                     /locus_tag="OQQ48_000877"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCS1951731.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(36628..37101)
                     /gene="trmL"
                     /locus_tag="OQQ48_000878"
     CDS             complement(36628..37101)
                     /gene="trmL"
                     /locus_tag="OQQ48_000878"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCS1951732.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
BASE COUNT         9248 a         8882 c         9297 g         9892 t
ORIGIN      
        1 acgcatggtt tacctccaga cgtttagcga gccagcgttg cgacaggcgg gtgagttctc
       61 ctttgcgctg ctcatattcc atgcccatat caattaacgt gatattggtg ctttcgagat
      121 aggcgaggtg ttcaagctgt gctgcgttca tgctgtcccg tggttcgcca gtaatgccat
      181 ttgcctgcgc ccactgcttc gccgtcatgc cacccagcac gatacgggcg atcatgttgc
      241 tctcgttgct gtagtggcgc tgctgcgtcg ttttgcccat ctcggcacgg gccatattca
      301 gcgcatcgca cattggctta aagttgtttg cggcactgag acgggctttt agctggcgac
      361 gatagcgggc ggcgatttca ggcacgctac gctgtaattc ttcctcgcac tggatgaaat
      421 aacggcggat agcgcggccc tgctcgttgc gttcaaccat tgccacttct ttagccatat
      481 ccagcgatag caggtaatca tgctctattt gctggcgagt ttttgcgctc gcccgttttg
      541 gcgtgctcaa attttcaaca cggataaaat cggttccggc aataaaaccg tactggtcaa
      601 ttcggccttt aatccagtta gtaaagtcgc ggcccacccc cagcgcttta tgcaacgcct
      661 tagcgctcac aatattggct tcacgcccgc caatgcgtcc ggcaataacg ggaacaatag
      721 cggcaaagtc gttgccgtta attactcccg tgttaacgtt gggttgcggg gcggcccccg
      781 aactgaatcg gcttttttcg atagtcatat tttcggctcc gttatgcggc ggtgagattg
      841 tccgggtata gattgagaat gtcggcaatt tcaggctgag aaagtccctg atagccgcca
      901 gcgtcggcgt tatggttcac aagccgaata acttttagaa cgtcaccgcg cccggtaaag
      961 cgatagcgta aatgtgaccc tatcccgtcg gggttctttt cgtctatacg ctcaagctga
     1021 atatcaagcc tgcgctctaa ctcgctggcg tagttccttc cagacgaaag gcggcaataa
     1081 cgcagaatgt cattttctgt ccatccctct ataccagtac gcagcatata gactctggca
     1141 cgatgctttt tcggtgtgcg ctttggtgct tgaagggatt ttgtaagggg cgtaacatca
     1201 gatacgcgaa tatctgaatt agccgcctgt aatgggcggt ttttcttttc catcaggcca
     1261 ccttcccgcg gcgttctgcc agccagttgt gaatctcaac cgcatcaaac gcagttattt
     1321 tttctcccag ctttaccgga cgtggtagtg aaccgtttct tactttacgg tcgatggtgg
     1381 atacactaac gcccagcaat tcagccaggc ggaagcgtct gatatagcct tttgtaggga
     1441 tagtgtcgat gttcatgatt cccgtaacct cttagcgata gttgttgctt cgttacgggt
     1501 atttaatggc gtgcacgctc aaaaaggaat gatcgtattt tttactatcg tgatagtaaa
     1561 atttactatc accttttttt ccctgccgtg gtgcgttctt ccggtgttct gtctgcgtcg
     1621 gctatgagtt tcttcaggta atctatggat ggctcatcat agccgccccc caaaagcgcc
     1681 cagtggttta ttgtggcgtt cgcccatgct tcgttggttt ccttcccgtt cttcccttta
     1741 cactcttctg ggtagtgccg cttcacataa atcaacgcca agaggcaaat ttcgcgcttt
     1801 tgcctgtggc gctctctgtt tccgtgctca acgtttttaa catcctcata aagagcttgt
     1861 aagtttatgc gagtttcggg gctgtatatg ggctggtttt tgtccatgca ttcgccgata
     1921 tattcaaccc acggccttgt tatgtacaaa tcctctacgg atatttcgtg gaaggcatgt
     1981 tttgtataat cgagaaaaaa ataataattc gcacttacgt ctagttctct ttttctgtgt
     2041 gcggggctga atatgcgtgc gggagtgtca atagaatcaa gccctgcgat gtcgagtata
     2101 taggaggcgt taacatgcca tagcccaagc acatggcaat ctatatgatt tacaacgcct
     2161 gccttaaagc ttttttcgtc ctctacttca gtgattatgt cggcaagtaa taagcagtct
     2221 tctttgacgt ctgggaataa agagcaattg cggtaaagac tcgcgatgct atgtaatgtg
     2281 ccgtcgtagc cgcaaaggta cgcgctcaaa tatatagatt ctgccgccgc cataatcaaa
     2341 agatcatcaa tatctatttc taataattcg gctgctctgt taagcctgaa aaaaggggcg
     2401 ggtggtaatt ttttcattgt gtcacctttt acgctgcgcc ttattttgtg attgagccag
     2461 gcggtaaggc ttcccgcttt tcgccccgtc gagctaggct caatctattc agttagttcg
     2521 cccttctgag aggcgttacg ttgtagtctt ttccgctttc cagtgccacc agcagatcag
     2581 cccactgtgc cagtgctgct ttacgctcat caaaatactg atggcggtta taaatcccct
     2641 ctacgccctt aatgcgatga ttgaggcatc gttccgccac aacaggatca acacccaacg
     2701 ctgctaaatg agtacgtgcg gtgcgtcgaa agtcgtgaat cgtgaaatta ggcacatccg
     2761 gcatttcggc gcgaacctta gccagtgcta cgggtaaagt gctttcctga atatgtggga
     2821 tcattctgtt ttgcatcttc ctggccggaa gcacccatgc gctattacat gaaaaggtgt
     2881 gtagctctct caaccattca acggctgggg aaggtagagg tatatcaata gggtctccgt
     2941 ttttgctgcg ttcttccggc aaatgccata ccgcaccatc taaatcaaac tcttcccatc
     3001 gtgcggcgca taattccatt ttgcggacgc ataacgctaa cagtaatttg aacgtgattt
     3061 cgttctggcg actgaatccc ttagccgtgc gcatggcttt gaatagctgg attaactcat
     3121 cgcgggttaa ccagcggtca cgggcagctt ctttccctcc ggcgtctgcc acctcaaagg
     3181 ctgaacaggg gttaatctct agcgcgtgcc gtttgattcc gtagtcgaat atgcggcgcg
     3241 tccatctcag cacgtccgtt gctatggtcg gcgctccacg gtcaacaata cctttcagca
     3301 tgtcatcgat atggcgcggt ttcacgtcct ctaccttcat gctgccaatg caggggttta
     3361 tatctttgtc gatacggcgg cggagtatat cggggtgctt ccagcgcggg aggatctgac
     3421 gctcaaagta ttcagcggca agctctgaaa cgcgcatagc gttcttctct gcttccatct
     3481 tcgccagtgc ttcggttttg cgcttctgct tctctcctgc aacgtcatag cccagcgcaa
     3541 cgcgagccga tagctctttg gctgtctctc tggcctttga tagtgatagc tcgctatacg
     3601 aaccaattag catggcgcgg gacttccctg cgagtttata gcggaatctc catgtggggg
     3661 ttttgtcggc ttcacggtaa cgtaggtata aaccgttacc gtctgcccgc ccctcgaatc
     3721 gctctccgct cttaatccat gcgcggatct gcatgtctgt tagttttggc attttgcgaa
     3781 tatccaaact ttgaataggg ggtgtaccca aagcaatgta cccaaacctc attgggtaca
     3841 cctttgtgta caccataatc atgcgctttg ttgcgtcttt atgatagccc atgataacaa
     3901 aaaagcccgc tgggttgcgg gcttggtgtg atttatgcgt tgtcctaaat gttgctgata
     3961 gcttactcga tattctgaat ctgctcgcgc atctgctcaa tcaacacttt cagctcaatc
     4021 gcggagtttg tcacttcggc attgatagac ttcgacgcaa gagtgttcga ctcgcggttg
     4081 aattcctgca tcataaagtc cagacgacga ccaacggctt cttttttctt cagaatgttg
     4141 taggtctctt tgacatgcgc ttccaggcgg tccagttctt cggcaacgtc aattcgttgt
     4201 gccagcagaa ccagctcctg ctccagacgg ttgttttcca actgaacctg agcatcttcc
     4261 agcttcgcga ccagacgctc acgctgccat tgcaggattt ccggcatatg ggcgcggact
     4321 ttgaccactt cggcggtgac gccttccaga cgctgctcga tcaatgcttt cagtgcctga
     4381 ccttcggttt cgcgcgcgac aataaagtcg tccagcgtac catccagcgc cgcgagaatt
     4441 tcagcggcaa tggcgtcaag atcctgctcc tgggctgcca tcacgcccgg ccagcgtaga
     4501 atatcaaccg ggttgatttc cccttcgtca ctctgcattt ttacccagtt cgcggcagtt
     4561 accagctgtt tagccagttt ttcgttgagg atcagctcac cttgcgcgct aatatctggc
     4621 tcatagcgca gggtacattc cactttaccg cgcgtcaggc gagaacgaat acgctcgcga
     4681 acgacaggtt caaggctacg gaactgctcc ggcagacgaa agtaagtttc cagataacgc
     4741 tggtttaccg agcgcatttc ccaggttgcg ctcccccatt cacccttgat ttcacgccgg
     4801 gcgtaggcgg tcatactgcg gatcatagac gttcctgttt ataaaaggag aggtggaagg
     4861 attatagcca tcgatgcctt gtcaggatag gaataaccgc cggaagtccg tataatgcgc
     4921 agccacattt gtttcaagcc ggagatttca atatgcgtcc agcaggccgt agcaataatc
     4981 aggtgcgtcc cgttaccctg actcgtaact atacaaaaca tgcagaaggc tcggtgctgg
     5041 tcgaatttgg cgataccaaa gtgttgtgta ccgcctctat tgaagaaggc gtgccgcgct
     5101 tcctgaaagg tcagggccag ggctggatca ccgcagagta cggcatgctg ccacgttcta
     5161 cccacacccg taacgctcgt gaagcggcga aaggtaagca gggtggacgc acaatggaaa
     5221 tccagcgtct gatcgcccgt gctcttcgcg cggcagtaga tttgaaagcg ctgggtgagt
     5281 tcaccattac gctggactgc gacgtgcttc aggctgatgg tggcacgcgt accgcgtcga
     5341 ttacgggtgc ctgcgtggcg ctggcagatg cgctacagaa gctggtggaa aacggcaagc
     5401 tgaaaaccaa cccgatgaaa gggatggtag ccgcagtttc tgtcggaatt gtgaacggcg
     5461 aagcggtttg cgatctggaa tacgttgaag actctgccgc agagaccgac atgaacgtag
     5521 tgatgaccga agacgggcgc atcattgaag tgcaggggac ggcagaaggc gagccgttca
     5581 cccatgaaga gctactcacc ttgttggctc tggcccgagg gggaatcgaa tccattgtag
     5641 cgacgcagaa ggcggcgctg gcaaactgat ttttaaggcg actgatgagt cgcctttttt
     5701 ttgtctgtag aaaagtaaga tgaggagcga aggcatgaaa ccatatcagc gccagtttat
     5761 tgaatttgcg cttggcaagc aggtgttaaa gtttggcgag tttacgttga agtccgggcg
     5821 caaaagcccc tatttcttca acgccgggct gtttaatacc gggcgcgatc tggcactgtt
     5881 aggccgtttt tacgctgaag cgttggtgga ttccggcctt gagttcgatc tgctgtttgg
     5941 ccctgcttac aaagggatcc cgattgccac cactaccgcc gtggcgctgg cggagcatca
     6001 tgaccttgac ctgccgtact gctttaaccg caaagaggca aaagaccacg gtgaaggcgg
     6061 caatctggtt ggtagcgcgt tacaaggacg cgtaatgctg gtagatgatg tgatcaccgc
     6121 cggaacggcg attcgcgaat cgatggagat tattcaggct aatggcgcga cgcttgctgg
     6181 cgtgttgatt tcgcttgatc gtcaggaacg ggggcgcggc gagatttcgg cgattcagga
     6241 agttgagcgt gattacaact gcaaagtgat ctctatcatc accctgaaag acctgattgc
     6301 ttacctggaa gagaagccgg aaatggcgga acatctggcg gcggttaagg cctatcgcga
     6361 agagtttggc gtttaaagaa actcgccgga tgaaaagtca tccggcgtca tattactgca
     6421 actgtgccgc aattagcggc cagcgggcgt caaaatcatc cgtcgggcgg tatttaaatt
     6481 cgctgcggac aaaacgtgac agcatacctt cacagaaggc caggatctgg cttgccagca
     6541 gggtttcatc ggtggtgtaa ccttcaccct cacgcattct cttttcacgc aatacctgac
     6601 gcagctgcgc ttcaatacgc tcgaacagct ggttgatgcg cccttgcagg cgatcctgtt
     6661 caaacattag cgcatgacca gtgaggatgc gggtcaggcc aggattacgc tcaccaaaac
     6721 cgagaagcag caacacaatc agacgcaggc gcgctgtggt gtctttctca tctttcagaa
     6781 tcaggttgat gcgagtaatc aggctatctt cgataaactc aatcaggcta tcgaacatgc
     6841 gggtcttact ggggaagtgg cgatacagtg ccgcttcgga aacgccgaca gaggcggcca
     6901 gttttgccgt cgtgatacgt tggcttccat cgctggattc cagcatcagc gccagagact
     6961 gaagtatttc ctcgcgacgg ttccttttcg cagtttgttt ttctgccatg ttacaaaata
     7021 cccctgaaaa taagcacttg ccaggcggca cccacgctat gaccgcaaac gaaatgtttg
     7081 cggctatgtt atgacgttat tcggatgcgt atgtgttact gacgaccaga gtgaccaaag
     7141 ccgccttcac cgcggtcggt ggcgtcgaaa tcttccacca gattaaattc agcctgtact
     7201 accggaacaa aaatcatctg ggcgatgcgt tcgccaggtt gaatggtaaa gctgtcctga
     7261 ccacggttcc acacggaaat catcaactgg ccctgatagt cggaatcgat caatcctacc
     7321 aggttaccaa gcacgatacc gtgcttatgt cccaatccgg agcgcggcag catcattgcc
     7381 gccagtgaag gatcggcaat atgaatcgcc agcccggtcg gaaccagcgt agtgtcaccc
     7441 ggagccagtt ctacggcgtc gtcgagacag gcacgcaggt caagtccggc agagccagag
     7501 gtggcataag tcgggagcgg aaattccttc ccaacgcgcg ggtccagaat cttaacgtcg
     7561 atttttttca tcataacggg tcacgatctc gtcgagtaat agttggccaa ggagctcttt
     7621 gcgctcaagc ggtaagactt tatctccgtc ctgccagaaa aggtgtaatg cgttgttttc
     7681 gctgttaaat ccttgagttg gctgggaaac atcgttcgcg cagatcagat caaggttttt
     7741 acggatacgt ttttgccggg cgtattcttc cacattattt gtttcggcgg caaatccaac
     7801 gacgtagggt cgatggtctt ttagtgcggc aacgcctgcg acgatatcgg ggtttttaac
     7861 catttttatt gttaattcat caccctgcgt ggcctgcttt ttgatttttt ctggggccac
     7921 ggtagctgcg cgataatccg ccacggcggc gcagccgata aaaatatttt gctgctgtac
     7981 agaagcattc acggcggctt ccatttccag cgcggtcatc acatcaacac gtttaacaaa
     8041 cggtggcgtc ggtagtgaaa ccggacctga taccagcgtg acgttcgcgc cacgacgggc
     8101 agcggcggcg gcgatagcaa aacccatctt gccggagctg tgattagaga tataacgcac
     8161 gggatcgagc ggttcacgcg tcggaccggc ggtaatcata atgttcagat gtttcaggtc
     8221 gttgacgggc gaaaaatgcg ccaccgccat atccacaatg gttaacggat cgagcattcg
     8281 cccaggaccg atatcaccac aagcctgact gccactgtct ggcccccaga tgagcaaacc
     8341 acgggaagca agcccctcta aattatgctg cgtggcagcg gcacggtaca tctgctggtt
     8401 catggcgggg agcacggcta caggcgcagg tgtagccaga caaatcgtcg ataccaggtc
     8461 attcgccatt ccggcagcaa cacgggcaat caaatctgcc gtggcagggg cgagaatcac
     8521 taaatcagcc catttaccca gctcaatatg gcccatagcg gcttcggctg ccgggtccag
     8581 caaactgtcg gaaacgggat aaccagaaac cgcctgcaag ctaagtgggg tgataaaggc
     8641 ttttgccgct tcggtcatgg ctacgcggac gtcggccccg cgatcgcgca aacgacgcac
     8701 cagttcaggg gttttatagg cagcaatacc gccgctaacg ccgagaacga tttttttacc
     8761 ggccaggctc atgatgattt ttcctgttgg gtgacaccag aagttggcga ttttatcaca
     8821 atacttttgt tgtcgtgcct tcacctgaga atcactttgc gaggcgcttt ccaggattga
     8881 aaactggccg tcgatttaac ggaacggcta tgacaggatg cgagcaccac aaaggaggtg
     8941 aaggtgaaaa acaattcaca gctgttgatg ccgcgcgaaa aaatgctgaa gtttggtatt
     9001 agcgccttaa cggatgtcga gctgctggcg ctatttctgc gtaccggaac gcgcggtaaa
     9061 gatgtattaa ccctggcaaa agagatgctg gagaatttcg gctctcttta tggcttgtta
     9121 acctctgaat atgaacaatt tagtggcgtt catggaattg gcgtggcgaa atttgcccag
     9181 ttaaagggga ttgctgaact ggcgcggcgt tactacaacg tgcggatgcg tgaagaaagc
     9241 cctttactca gcccggagat gacgcgggaa tttttacaaa gccagcttac gggtgaggag
     9301 cgggagatct ttatggtgat ctttctcgac tcacaacacc gggttataac gcatagccgt
     9361 cttttttccg gcacgctaaa ccatgttgaa gtccatcctc gggaaattat ccgcgaagcg
     9421 ataaaaataa acgcctcggc gctgatcctt gcacataatc acccttcggg ttgtgctgaa
     9481 cccagtaaag cggataaact cattactgaa cggataataa agagttgtca gttcatggat
     9541 ttacgcgtgc tcgaccatat cgtgattggg cgtggagagt atgtttcttt tgccgaacgc
     9601 ggctggattt aacccgctat gcgcgatcct tcgggatctt tgtctgttcg ggacttgagc
     9661 acatcgctga gtcagcgtat actacgccac ctttgagaat ctcgggtttg gcatttgggc
     9721 ctggcaatcg agagttcaca tagaactgcg atgactgggc tgtaaagcct gacgaggcgc
     9781 caatacccca tacgaagctc gagctaattt gatttttgga gaatagacat gtcccgagtc
     9841 tgccaagtta ctggcaagcg tccggtgacc ggtaacaacc gttcccacgc actgaacgcg
     9901 actaaacgcc gtttcctgcc gaacctgcac tctcaccgtt tctgggttga gagcgagaag
     9961 cgttttgtca ccctgcgcgt atctgctaaa ggtatgcgtg taatcgataa aaaaggcatc
    10021 gatacagttc tggctgaact gcgtgcccgt agcgaaaagt actaagtact tagaggaaat
    10081 aaatcatggc taaaggtatt cgtgagaaaa tcaagctggt ttcttctgct ggtactggtc
    10141 acttctatac cactacgaag aacaaacgta ctaagccgga aaaactggaa ctgaaaaaat
    10201 tcgatccagt tgttcgccag cacgtgatct acaaagaagc gaaaatcaaa taattctcgc
    10261 tttgatgtaa caaaaaacct cgctccggcg gggttttttg ttatctgctt gcccccatat
    10321 tgactgcatc tgttcattcc tggagatgct atgcctgaat tacccgaagt tgaaaccagc
    10381 cgccgcggca tagaaccgca tctcgttggt gcaaccattc ttcatgcagt ggtgcgcaac
    10441 ggacgcttgc gctggccggt ttcagaagag atctaccgtt taagcgacca accagtgctt
    10501 agcgtgcagc ggcgggctaa atatctgctg ctggagctgc ctgagggctg gattatcatt
    10561 catttaggga tgtctggcag cctgcgcatc cttccagaag aacttccccc tgaaaagcat
    10621 gaccatgtgg atttggtgat gagcaacggc aaagtgctgc gctacaccga tccgcgccgc
    10681 tttggtgcct ggctgtggac caaagagctg gaagggcata atgtgctggc ccatcttgga
    10741 ccggagccgc ttagcgacga tttcaatggt gagtatctgc atcagaagtg cgcgaagaaa
    10801 aaaacggcga ttaaaccgtg gctgatggat aacaagctgg tggtaggggt agggaatatc
    10861 tatgccagcg aatcactgtt tgcggcgggg atccatccgg atcggctggc gtcatcactg
    10921 tcgctggcag agtgtgaatt gttagctcgg gtgattaaag cggtgttgct gcgttcgatt
    10981 gagcagggtg gtacaacgct gaaagatttt ctgcaaagtg atggtaaacc gggctatttc
    11041 gctcaggaat tgcaggttta cgggcgaaaa ggtgagccgt gtcgggtgtg cggtacgccg
    11101 attgtggcga ctaaacatgc gcagcgggca acgttttatt gtcggcagtg ccagaagtaa
    11161 ttcatgcgcg ccggatcgca taccatccgg cataaacgct acgctaactt cgccatcagc
    11221 gcctgatgga cattctccgg caggaaatgg gtgacatcgc cctgatggcg cgccacctct
    11281 ttcaccaacg atgaagagat aaacgaccac tctttcgacg gcatcagaaa cacactttcc
    11341 agttccggca ttaagtggcg attcatatgc gccagctgca tttcatattc aaaatccgcc
    11401 accgcacgca ggccacgaat cagcaccgta gcgtgttgat tacgggcgaa gttcgccatc
    11461 aaatcactaa acccaactac ttccacgttc cccagatgcg cggttgcctg ctgtgccagc
    11521 gccacacgct cttccagggt aaacatcggt tttttactgg ggctggcggc aatcgccaga
    11581 ataacgtgat cgaacatttg cgtggcgcgc gtcacgatat cgatatgacc attggtaatg
    11641 ggatcgaaag tacccggata aatcgcccgt ttttgcataa caacctcaat gcgttttcgg
    11701 tggcaggtaa ggttccagca gttgaagcag gcgctgtagc gcgccctggt tttgatacag
    11761 tacttcaacg gcatgacggc catagaaact acggtaatcg gcgtcggtga gtaaagagga
    11821 aacctctttt gcaagcgtag tggcatcggt aacggtaatc agcccgcttg cctgctccag
    11881 ccgcgcgcaa atgtctttaa aattaaaagt atgcggcccc atcaataccg gaatagcgtg
    11941 tgcggcagct tccagcggat tatgcccacc acgttcaacc agtgaaccgc caacaaaggc
    12001 gagatcggca atgccgtaca gtaacatcaa ctcgcccatc gtatcgccaa ccacaacctg
    12061 cgtgctggtg gaggggactt cccctgaaga gcgtgtgata tagcttagtc cagcctggcg
    12121 gacaaggtta atcgcatccg ggaagcgttc tggatgacgg ggtaccagga tgagcaataa
    12181 attcgggaat tgctgtaaca atacctgatg tgcggcgatc accacactct cttcaccttc
    12241 gtgagtgctg gtggcaatcc ataccgggcg gtgtggtgcc cactggcggc gcagcgtcac
    12301 ggctttagca gccaactgcg gcgttacaga aatatcgaat ttcaggctac cggtaacggt
    12361 tacctggtta ttttttgcgc ccagcgccac aaaacgtgca ccatcttctt cattttgggc
    12421 ggcaatcagc gtaatacgac gcagcaagcg acggacgaat ttacccagtt tggcataacc
    12481 tgcggccgag cgggcagaga gtcgcgcgtt cgcgatcacc agcggaattt tacgtttatg
    12541 tagcgccgca atcaggttag gccatagttc ggtttccata atcaacacca gtttagggtc
    12601 gactttattc aggaaacgat taagtgcatc gggcaggtca tacggcagat aaacgtgctg
    12661 aacatccttc ccgaaagccg attgtacgcg ctccgaaccg gttggcgtca tggttgtaac
    12721 ggtaatcggt aaatcaggat aacgatgacg cagcgcgcgc accaacggga tcgccgccag
    12781 agtttcaccg acggagacgg agtgcagcat aatgccgcct ggttttagcg gatggcggta
    12841 aaaaccgtaa cgttcacccc agcgttttcg ataggccgga gccttacgtc cgcgcaccca
    12901 gagccgtatc cagatcagcg gctgaataag gtagagaagg gcggtgtaaa gcaattcgag
    12961 catagtaaat agctgactta tggatgtgct ggggattcta tgtatttagc tgtggcttta
    13021 ccattacttt tcccgttttt gacttaaata gcaatttacc cgccaattct tgctgaacat
    13081 ctatactttt tttgtatttt tatgcgattc attgatttct gctgtacgtg caggaaggaa
    13141 tcgcagagca acagtgaaag aatatggttg ctaatgattt tatttgattg atttctttat
    13201 tgaaaatcaa caatgtgctt aaaaatcatt cgggtcggat tgccgcgaaa gaagtgatac
    13261 actagcgcgt caatatcagt gcggtatcag tattcaggta gctgttgagc ctggggcggt
    13321 agcgtgcttt ttctgcttca ccttaatcgg ataatctcaa caaaaagagt tacttgtgga
    13381 taagctattt cgaagaattt tgctcattaa gatgcgtttt catggggata tgttattaac
    13441 tactcccgtc attagttcgc tgaaaaaaaa ttaccctgac gcaaaaatcg atgtgctgct
    13501 ttatcaggac accatcccga tcctgtctga aaatccagag attaacgcgc tctacggcat
    13561 aaaaaataaa aaagcaaaag cctcagaaaa aattgccaac ttttttcatc tcatcaaggt
    13621 attacgtgcc aataagtatg accttatcgt caatctcacc gatcaatgga tggttgctat
    13681 actggttcgc ttattaaatg cccgtgtgaa aatttcccag gattatcatc atcggcagtc
    13741 tgctttttgg cgtaaaagtt tcacccattt ggtgccgttg cagggtggaa atgtggtgga
    13801 aagtaactta tccgtgctga ccccattgcg agttgattcg ttggtgaagc agacaaccat
    13861 gagttacccg cctgcaagct ggaaacgtat gcgtcgcgaa cttgatcatg ctggtgttgg
    13921 acaaaattat gtggttatcc aacctacggc gcggcaaatc ttcaaatgct gggacaacgc
    13981 caagttttcc gctgtgattg atgccttaca tgctcgtggt tatgaagttg ttctgacgtc
    14041 cggcccagat aaagacgatc tggcctgcgt caatgaaatt gcgcagggat gccagacgcc
    14101 accagtaacg gcgctggctg gaaaggtgac cttcccggaa cttggtgcgt taatcgatca
    14161 tgcgcagctg tttattggcg ttgattccgc accggcgcat attgccgctg cagttaatac
    14221 gccgctgata tcgctgtttg gtgcgacaga ccatattttc tggcgtccct ggtcaaataa
    14281 catgattcaa ttctgggcgg gagattaccg ggaaatgcca acgcgcgatc agcgtgaccg
    14341 aaatgagatg tatctttcgg ttattccggc ggcagatgtc attgctgctg tcgataaatt
    14401 actgccctcc tccacgacag gtacgtcgtt atgatcgttg ctttttgttt atataaatat
    14461 tttccctttg gcggtttgca gcgcgatttt atgcgtattg ctcagacagt cgccgcccga
    14521 ggtcatcatg ttcgggttta tacccagtcg tgggaaggcg aataccctga tgtatttgaa
    14581 ctgatcaaag tgccggttaa atcgcatacc aatcacgggc gcaatgcgga gtattttgcc
    14641 tgggtgcaaa aacatttacg cgaacatccc gtcgataaag tcgttggatt caacaaaatg
    14701 ccggggctgg acgtttatta tgccgctgat gtttgttatg ccgagaaagt cgcgcaggaa
    14761 aaaggctttt tctatcacct gacgtcacgt tatcgccatt atgccgcctt tgagcgggca
    14821 accttcgaac agggcaagcc gacacagctg ctgatgctga cagataagca aatcgccgat
    14881 ttccagaaac attatcagac tgaagcggag cgttttcata ttctgccacc ggggatttat
    14941 cctgatcgta aatatagcca gcagccagct aatagccgtg aaatcttccg taagaagaat
    15001 ggaataaccg aacaacaata tttattgttg caggtcggtt cagacttcac gcgtaaaggt
    15061 gtcgatcgtt ccattgaagc acttgcttcg ttaccggatt cgctgcgcca caacacattg
    15121 ctatatgttg ttggacagga taaaccgcga aaatttgagg cgctggcaga aaaacgcggc
    15181 gtgcgcagta atgttcactt cttctcgggg cgcaacgatg tctcggaatt aatggcggcg
    15241 gcggatttat tactgcatcc tgcctaccag gaagcagcgg gaattgtgct actggaagcg
    15301 attactgcag gattaccggt actaacaaca gccgtttgtg gctatgcgca ttatattgtc
    15361 gacgccaatt gcggcgaggc tattgctgag ccattccgcc aggaaacatt gaatgagatt
    15421 ttacgcaaag cgttaacgca atcttcattg cgccaggcct gggcggaaaa tgcgcgacat
    15481 tatgctgata cacaagattt atacagtctg ccagagaaag cggcggatat cataacgggt
    15541 ggtctggatg gttgaactta aagagccgtt tgccacgtta tggcgcggta aagatccttt
    15601 tgaggaagtt aaaaccttgc agggtgaggt ttttcgtgaa ctggaaactc gccgcactct
    15661 gcgatttgaa atggcgggca aaagctattt tctcaaatgg catcgcggca cgaccctgaa
    15721 agagataatc aaaaatttac tctcattgcg gatgccagta ttaggcgcag accgcgaatg
    15781 gagtgcgatt catcgactgc gggatgtcgg cgttgatact atgtatgggg tggcatttgg
    15841 cgaaaaaggt attaatccgc tgagcagaac ctcgtttatt attaccgaag atctgacccc
    15901 aaccatcagt ctggaagatt actgtgctga ctgggcgacc aacccaccag atgttcgcgt
    15961 aaagcgtatg cttattaagc gtgtcgcgac gatggtgcgc gatatgcatg ctgcgggcat
    16021 taaccaccgt gactgttata tctgtcattt cctgctgcac ttgccttttt ccggtaagga
    16081 agaggagtta aaaatttcgg taattgacct acaccgggcg cagcttcgca cgcgcgttcc
    16141 acgtcgttgg cgcgataaag atcttatagg gctttatttt tcttcaatga atattggcct
    16201 gactcagcgg gatatctggc ggtttatgaa agtgtatttt gccgccccgc ttaaagacat
    16261 tctcaagcag gaacaaggac tgctgtcgca agcagaagca aaagccacaa aaatcaggga
    16321 aagaacgatt cgaaaatcgt tgtaatttcc ggagagaaat atgagtgccc actattttaa
    16381 tccacaagag atgatcaata agacaatcat cttcgatgaa aggccagcgg cgtcagtggc
    16441 atcatcattc catgttgctt atggcattga taaaaacttt ctttttggtt gtggtgtttc
    16501 aatcacgtca gttttgttac ataacaacga cgtgagtttt gttttccacg tttttattga
    16561 tgatatccct gaagccgata tccagcgttt agcccaattg gcgaaaagct atcgtacctg
    16621 tatccagatc catctagtaa attgtgaacg gcttaaggca ttaccgacga ccaaaaattg
    16681 gtctattgcc atgtatttcc gttttgtaat tgcagattac tttattgatc aacaagataa
    16741 gatcctttac ctggatgctg atatcgcctg tcagggaaac ttaaagccgc tgataacaat
    16801 ggatcttgcc aataacgttg ctgctgttgt tactgaacgc gatgctaact ggtggtcgtt
    16861 acggggtcaa agtctgcagt gtaatgaact tgaaaagggc tactttaatt caggtgtcct
    16921 gttaattaat acactagcgt gggcgcagga gtccgtttct gctaaagcga tgtcgatgct
    16981 tgctgataaa gccatcgttt cccgtttcac ctatatggat caagatatcc ttaatcttat
    17041 cctgttaggg aaagttaaat tcattgatgc taaatacaat acgcaattta gtttaaatta
    17101 tgaattaaaa aaatcatttg tttgtccaat taatgatgaa accgtattaa ttcattatgt
    17161 cggcccgaca aaaccctggc attactgggc cggttatcca agtgcgcaac cttttatcaa
    17221 agccaaagaa gcatcgccct ggaaaaatga accgttaatg cggccagtta actcaaacta
    17281 tgctcgttat tgcgccaagc ataattttaa acaaaataaa ccaattaacg ggataatgaa
    17341 ttatatttat tatttttatt taaagataat aaaatgaaat tgagaaaagt taaatgaatg
    17401 aatttataaa agaacggttt tcgtatttag cagataataa aaaagaaaac gccccagagc
    17461 taaatgtttc ctacggtatc gataagaatt ttttgtatgg tgctggcgtt tcaatttctt
    17521 ccgttttgat taataattca gatattaatt ttgtctttca tgttttcact gattatgtgg
    17581 atgatgatta tttaaagtca tttaatgaaa cagcaaaaca atttaatacc tcaattattg
    17641 tatatttaat tgaccccaaa tactttgctg atctgccgac gtcacagttt tggtcatacg
    17701 cgacatactt cagggtattg tcttttgaat atctgagtga aagtatttcc acactgctgt
    17761 atctggatgc cgatgttgtt tgtaaaggaa gcctgaaacc tctcacagaa attatattta
    17821 aagatgagtt tgctgcggtc attcctgaca atgatagtac tcaggcggca tgtgcaaaac
    17881 gcctcaacat tcccgaaatg aatggacgtt atttcaatgc aggcgttatc tatgtcaatc
    17941 ttaaaaaatg gcatgaagca aatttgacac cgtatttact caaactttta cgaggggaaa
    18001 ctaaatatgg ctctcttaaa tatttagatc aggatgcgtt gaatatcgca tttaatatga
    18061 ataatatcta ccttgcgaag gattttgata ctatttatac cctgaaaaac gaactttatg
    18121 atcgtagtca tcgaaagtat cagcaaacca ttaccgataa aacagtgttg attcactata
    18181 cagggataac taaaccatgg catagctggg ctggatatcc gtctgcatca tactttaata
    18241 tcgcgcgtga acaatctccc tggaagaaat atcctcttaa agaggcgcgg actgttgcag
    18301 aaatgcagaa acaatataag catctgtttg cccatggtga gtatataaaa ggcataactt
    18361 cattaattaa gtacaagctt aagaaataaa ttactcttat gattacaagt atacgttatc
    18421 gcggcttctc attttattac aaagataatg ataataaata taaagaaatc tttgatgaaa
    18481 tactggccta taattttaaa acagtaaaag tattatgtaa tattgatgat accaaagtat
    18541 cgttgattga tacgaaatat ggtcgctatg tttttaaagt ctttgctccg aaaacgaaaa
    18601 ggaatgaacg ttttttaaaa tcttttgtaa aaggtgacta ctatcagaat ttgatcgttg
    18661 aaaccgatcg tgtcagaagt gcaggtctta cttttcctaa tgatttttat tttctggctg
    18721 agcgtaaaat tttcaactat gccagtgttt ttatcatgct tatcgagtat gtcgagggcg
    18781 tagagctcaa tgatatgccg attattccag aaaatgttaa agcagaaatt aaggcttcga
    18841 tggaaaaatt gcatgcctta aatatgctat cgggcgaccc acaccggggt aactttatcg
    18901 ttagtaaaga tggtgtcagg ataattgacc tatccggtaa aagctgtacg gcagagcgta
    18961 aagctcgtga tcgtctggcc atggaacgtc acctgggtat tgccaacgaa ataaaagatt
    19021 atggctacta ttcagtaatc tacagaacca aactacgcaa atttattaaa aaattaaaag
    19081 gcaaagcgta aaccacacag tcaaaacgga accaacatgg atttattagc agagagtatt
    19141 actgaagtcg ctgtctctgg ggaaattgct aacaccgatc gtgtgttaaa tatcgcttac
    19201 ggtattgacc gtaacttttt atttggtgcg gcagtatcta tgcaatcagt tgttatgcat
    19261 aacccggacc ttgcggttaa gtttcatctc tttaccgact acattgatga agattatcta
    19321 caacgtgtta atgcttttac cagcaaaaat gctaacgttg aagtaataat ttataaagtc
    19381 tccaatgcct ttattgatat cttccccagc ctgaaacagt ggtcttatgc aacattcttc
    19441 cgtttagttg cgttccagta tttgagtgaa actattgaaa atctgttata tatcgatgct
    19501 gatgtcatct gtaaaggctc attagctgga ttgcttgata ttaattttga tgaggataag
    19561 ttcgcagctg ttattaaaga tgtgcctttt atgcaggaaa aaccagcgaa gcgtctggct
    19621 atagagggac ttccagggaa ttatttcaac gccggtgtag tatatctgca gcttgaagca
    19681 tgggcgaaaa atgattttat gaataaagcc attgctatgc tggcaagtga cccgcagcac
    19741 acgaagtata aatgccttga tcaggatatt ttaaatattc tgttctttgg tcattgtaat
    19801 tttattagcg gcgattatga ttgcttttat ggcattgact atgagttaaa aaataaaagc
    19861 gatgaagatt ataaaaagac cattaccgat gatactaagc tgattcatta tgttggcgta
    19921 acgaagccct ggaacgactg gacgaattat ccctgccaga agtattttaa tgaggcttat
    19981 caggcttctt gctggaatga tgtggcgttt attccagcca cgaatgaaaa gcagtatcaa
    20041 gtgaaatatc aacatgcaaa gaaaaatggt gatacgttta acgcttttat ttacttcatt
    20101 aaatttaaat taaataagta taaaagaaaa ctatttggat aataataaaa atcatggatg
    20161 tctgagttac aataataatt aaggatgagt actatcatat tttattagtt caggcaagag
    20221 aacacgatga gcaatgatta ccctttagta tctataataa taccgacgta taattcatct
    20281 gattacatta ctgaaactct aacgaaatta gaaaaacaaa cttacccaaa ttttgaaatt
    20341 gttattgtta atgatggttc taaagataac acatcaaacg ttttgagaga gtatgggtta
    20401 acccactctc gattaattat tgtcaataaa gaaaatggcg gtgtttcgtc tgccaggaat
    20461 acaggtatcc gcaaggcgca aggacagttt atatgtttta tggatgatga tgatgagata
    20521 gatcctaact atctgctgaa gatgtatacc agacaacatg agacgggagg ggatgcgatt
    20581 tattgtgggc tttatggcca ttatataaaa gatggtgtta cttactcacc tataaataca
    20641 gagtttaatg aaggatcttt acttttcgac tttttttata aaaaggttag attccatata
    20701 gggtgcttgt ttataagaaa acaacttctg gaagagaata atcttttttt tgatgaagat
    20761 ttacgactag gagaagatct tgattttatc tatcgactgc taattacatg cgatatgtat
    20821 gcggttccat attatatgta taagcataac tatagagaaa attccttaat gaactcatgt
    20881 agaaccatca ctcattatcg acatgagtca tttgcgcacg aaagaatcta ctcttctgtg
    20941 atgcagttat acaaaggtaa ccggaaagaa gaaattcata cattattgag tcaaaataga
    21001 gcttatcata aaactcgtta tttatggaat gttctactta atggtgattt taaactattg
    21061 aatcaattag ttgaaagcaa tgaaaaagaa ttaagatatt gtaatcttcc tggcaagaga
    21121 gataagagac gagcaaaaat attagcatca aaaaattata ttctctggag gatggtaaga
    21181 ctggtaaata gaaaaaagaa taaacgttag atcttgcatt aaataaccca tcctcgtagc
    21241 ataggttgaa attatatgtc gttttgttgg aatgaaatta attctggtat aaagtcttta
    21301 attcttatat tatgtatttt ttctttaatg actttgtctt tatgggatga tgttgcaaca
    21361 aagtttcttc atgcagctgg aattatatct gcattgtatt ttcttgcgac accaaaaaaa
    21421 acaataacta ataatcctac tttgttaatt ttcatctcat tatgtctttt gggtatcgta
    21481 aatatcatct ggtattcaca ttataaagtt tcaggctctg tttataccaa tgcatatcgt
    21541 ggcccaatgg aaactggaaa aattgccttg tgtagcgctt ttattttctt agttcttttt
    21601 gctaaaaatg agatgagaac aaaaataaaa tttgggaaac taattctgtt cgcatccctg
    21661 gcaacgcagt tacttttttt tgcgcatgcc atgtggcaac atttctattt aaacgtcgac
    21721 cgtgttgcat tatcagcttc ccacgctaca acagcaggct acatcatcct ttttccttct
    21781 ttactggcat caattctcat tttaaaatcc gactttagac ataaaacaac attatataca
    21841 attaacttca tgcttagctt atgtgctgtc atagtaactg agacgcgtgc agccatatta
    21901 gtgtttccat tctttgcgtt aatattaatc gtaatggata gttatattaa taagcgaatt
    21961 aattataagt tatattgttt tattgcgatt gcattattag caggtgtatt ttcttttaaa
    22021 gatacattgc ttatgagaat gaatgactta aataacgatt tagttaatta ttcgcatgat
    22081 aacaccagaa cttcagtcgg tgcccgtctg gcaatgtatg aagttggctt aaaaacatat
    22141 tctccaatag gacaatcact ggaaaaacgt gcagaaaaaa tacatgagct agaagaaaaa
    22201 gagcctagat tgagtggcgc tttacctttt gtagattctc atttgcataa cgatctcata
    22261 gatacgttat caacgcgtgg tattcctgga gttgtattaa caattttagc attttcagca
    22321 atactcatat atgccttaag aactgctaaa gaaccttata ttttaatctt gcttttttca
    22381 ctactggtag taggcctaag tgatgtaata ctcttttcta aaccggttcc gactgctgtg
    22441 tttgtcacca taatattgct ttgtgcttat tttaaagcac aatcagacca atatttatta
    22501 gataagtaat gaatatatga aataaaatca gcaaggaggc tgattttatt tcaaaattat
    22561 ctattaaggg gttgtaattt aaatcatagc agcgttttca gcgttttctt caatgaatcg
    22621 cttaacagca tttgctgtca attgagacaa ctcattcccc ggagccctac aaaccatttg
    22681 attcttccca tacccaccaa ttaatcccgg atcggttggt ccataaaccg tgatattggg
    22741 tctatccagt gccgccgtta aatggcttaa ccccgtatcc accgacacta caaatttagc
    22801 cccggccagc acgcgggcaa cgccttccag actcatcttc ggcaatactt caacataatc
    22861 aaatccttcc gccagtcgtt tcgcccgttc ttcctcatgc ggcgcgcccc acggaagttt
    22921 aatccgtatt cctgaatcag ccagtaaacc aatcaattct cgccagtgtt cttccggcca
    22981 gtgtttatca tcacgggtcg tcgcatgaag aaatacggca tattcgccag catctgtagg
    23041 caggttcgtc agaaaatgct gtgcgatagc ataatcgccc tgggtttgcg gtttgctata
    23101 gcccaaactt ttggcaaaca gttcgcgggt gcgttctacg gcgtgctgct gttttgcaat
    23161 atgatgctta caattgtaaa acaggctggc taaaggttcg cgagcggttt gccagtccaa
    23221 tccatgcttt acgccatgcg ccagacgtgt caccagtgcc gcgcttttta ccagcccctg
    23281 agcgtcgata actgcgtcat agttctctgc ttgtagcgct tcacgaaacg ctttgcgttc
    23341 agcttttatg ggggccgaga accaggcttt acgccagcga cgtattgcca caggaataac
    23401 tcgctcaacg gcagcgtgcc aggaaggaat ctgtgcgaac ccttcttcca ccacccagtc
    23461 aaacttaatc cctgggattg cctgctgggc atcagtgagt gcgggcaacg tatggagaac
    23521 atcgcccatc gacgatgttt taacgatcaa aacccgcatc cgtcaggctt cctcttgtaa
    23581 caatagcgtg ttgagttctt ccagtacgcg ctggggagta atgtcgatca agctctggtg
    23641 ataaccctcc gcagcgtcac ctttacgcac tttgtgatag ccggtaatca gacggatcac
    23701 gcgcgcttta tgggatagcg gcggtgtgaa gtccgggcta cttggaccat acagggcaac
    23761 cagcggacga ttgagcgccg ccgcaacgtg catcaggcca gaatcgttag tgacaatggc
    23821 tttacaggct gcaatcagga taaccgcttg atcaagctgt gtttcccccg ccaggttccg
    23881 acaccatgcc tgctgctcgg tattcaaagc ggcaagaatc tcattgcccg cttcatgatc
    23941 tttcgcagag ccaaacagaa ccacctgata accttcatca atcagctgct ttgccagttc
    24001 cgcatagtgg tagtgtggcc agcgttttgc cggaccaaac tccgcacccg ggcaaaagcc
    24061 aatcatcgga cgttctgatg aaagcgaaaa ttgattacag gtatatgatt tttcaccttc
    24121 gctcacctgc aactgcggcc ataacaatgg ctgcggcaga tcttgtgcgg tacgcataat
    24181 gcctttgtca taggccagcg cgacatagcg ttccaccatt agcggccagg cttctttatc
    24241 gagcacgcgt acatcgttga gtaaaccgta gcgcatctcg ccgcgccagc cggtgcgatg
    24301 aggaataccc gcgaagaaag gcactaatgc agatttgaag gagttgggta agacgtaggc
    24361 gcggtcgtag cgcttttcac gcaggctatg acccagtttg cggcgttcgc cgatttccag
    24421 cgctccgtga ccgagaggca tagggatagc ttcgttaact tccggcatcc gcgataataa
    24481 tggacggcac catgccggtg ccatcacatc gattatcgcc tggggatagc gcgcctggag
    24541 cgtgcgatag agactttgcg acatcatcat gtcgccaacc caagacgggc cgatcaccag
    24601 tattttcatg cagagctctt atgcgtcgcg attcagccag gccatgtatt ccgttacacc
    24661 ttcagcaacg gttttgaacg gtttgtcgta acccgccgcg cgcagatttg tcagatctgc
    24721 ctgagtgaac gcctggtagc ggcctttcag cttatccggg aacggaatgt attcgatctg
    24781 gcctttcttg tgataagcca gagtagcatc tgctacggcc tggaaggatt ccgcacgacc
    24841 agtaccgagg ttgaagatac cggaaacgcc attttccagg aaccacaggt ttacatctgc
    24901 cacgtcgccc acatagacga agtcacgttt gaagttctcg ctaccttcaa acagcttcgg
    24961 tgattcaccg ttgttaagct gagtgttgag atggaaagcg acgctcgcca tgctgccttt
    25021 atggccttca cgcggtccat aaacgttgaa atagcggaag ccaacaatct gcgagttcgc
    25081 ttcaggcagg atttggcgaa catattcatc aaacaggaat tttgagtaac cgtagacgtt
    25141 caacggtttt tcgtactcgc gggattcaat aaagtcggag gtgcgtccgc cgtaggtggc
    25201 tgcggaagaa gcgtacagga acgggatttc gcgctccagg cagtagtgca gcagctcctt
    25261 ggagtattga tagttgttat ccatcatata cttgccgtcc cactcggtgg tggaagagca
    25321 tgcgccttcg tggaaaatcg cttcgacatc gccgaactct tcgccagcca taatctggat
    25381 caggaagtct tccttatcca tatagtccgc gatatccaga tccaccaggt tcacaaactt
    25441 ggtgccgtct ttcaggttgt ccaccaccag aatatcggtg atgcctttat cattcagggc
    25501 cttaacgatg ttgctgccga taaagcccgc gccgccggta acgatgatca taactgtaac
    25561 cttcgaatta tggagtcaga gacaatctca gacacgaata cttctatcat atcactatac
    25621 ggcgttggct tcagccattc gccaaaagtg agattgatgg acgttattta tcctgaattt
    25681 tgcagaagtg ttaacgcgtc atctcgtcgc gacgtataag tttgggtaat atgtgctgga
    25741 atttgccctg tctggagaat cgcaatgcgt ggagaatttt atcagcagtt aaccaacgat
    25801 ctggaaaccg cacgggcgga agggttgttt aaagaagagc gcattattac gtctgcgcag
    25861 caagcagata tcactgtggc tgatggaagc cacgtcatta acttttgtgc caacaactat
    25921 ctcgggctgg cgaatcatcc ggatctgatt gcggcagcaa aggcgggaat ggattctcac
    25981 ggtttcggca tggcttcggt gcgttttatt tgcggcactc aggacagcca taaagagctt
    26041 gaacaaaaac tggcggcatt cctggggatg gaagatgcga ttctctactc ttcctgcttt
    26101 gatgctaacg gtggactgtt tgaaacgctg ctgggtgcgg aagacgccat tatctccgac
    26161 gcactgaacc acgcgtctat tattgatggt gtgcgtctgt gcaaagctaa acgctatcgc
    26221 tatgccaaca acgatatgca ggagctggaa gcacgtctga aagaagcgcg tgaagccggt
    26281 gcgcgtcatg tgctgattgc caccgatggt gtgttctcaa tggacggcgt gattgccaac
    26341 ctgaaaggcg tttgcgatct ggcagataaa tacgatgcct tggtgatggt ggatgactcc
    26401 cacgcggtcg gttttgtcgg tgaaaatggt cgtggttccc atgaatactg cgatgtgatg
    26461 ggccgggtcg atattatcac cggtacgctt ggtaaagcgc tgggcggggc ttctggtggt
    26521 tataccgcgg cgcgcaaaga ggtggttgag tggctgcgcc agcgttctcg tccgtacctg
    26581 ttctccaact cgctggcacc ggccattgtt gccgcgtcca tcaaagtact ggagatggtc
    26641 gaagcgggca gcgaactgcg tgaccgtctg tgggcgaacg cgcgtcagtt ccgtgagcaa
    26701 atgtcggcgg cgggctttac cctggcggga gccgatcacg ccattattcc ggtcatgctt
    26761 ggtgatgcgg tagtggcgca gaaatttgcc cgagagctgc aaaaagaggg catttacgtc
    26821 accggtttct tctatccggt cgttccgaaa ggtcaggcgc gtattcgtac ccagatgtct
    26881 gcggcgcata cccctgagca aattacgcgt gcagtagaag cattcacgcg tattggtaaa
    26941 caactgggcg ttatcgcctg aggatgtgag atgaaagcgt tatccaaact gaaagcggaa
    27001 gagggcatct ggatgaccga cgtccctgta ccggaactcg ggcataacga tctgctgatt
    27061 aaaatccgta aaacagccat ctgcgggact gacgttcaca tctataactg ggatgagtgg
    27121 tcgcaaaaaa ccatcccggt gccgatggtc gtaggccatg aatatgtcgg tgaagtggta
    27181 ggtattggtc aggaagtgaa aggcttcaaa atcggcgatc gcgtttctgg cgaaggccat
    27241 atcacctgcg gtcactgtcg caactgtcgt ggcggtcgta cccatctgtg ccgcaacacg
    27301 atcggcgtcg gcgttaaccg tccgggctgc ttcgccgaat atctggtgat cccggcgttc
    27361 aacgccttca aaatcccaga caatatctcc gacgacctgg cttccatttt tgatcccttc
    27421 ggtaacgccg tgcataccgc gctgtcgttc gatctggttg gcgaggatgt gctggtctct
    27481 ggtgcaggtc cgataggtat tatggctgcg gcggtggcga aacacgttgg tgcacgcaat
    27541 gtggtgatca ctgatgttaa cgaataccgc cttgagctgg cgcgcaaaat gggtatcacc
    27601 cgtgcggtta acgtcgcgaa agaaaacctt aatgatgtga tggctgaact gggcatgacc
    27661 gaaggctttg atgtcggtct ggaaatgtcc ggtgcgccgc cagcgtttcg taccatgctt
    27721 gacaccatga accacggcgg ccgtattgcg atgctgggta ttccgccgtc tgatatgtct
    27781 atcgactgga ccaaagtgat ctttaaaggc ttgttcatta aaggtattta cggtcgtgag
    27841 atgtttgaaa cctggtacaa gatggcggcg ctgattcagt ctggcctcga tctctcgccg
    27901 atcattaccc atcgtttctc tatcgatgat ttccagaagg gctttgacgc tatgcgttcg
    27961 ggccagtccg ggaaagtaat tctgagctgg gattaacacg aacaagggct ggtattccag
    28021 cccttttatc tggggataat ctgttgaata tgtaaaatcc tgtcagtgta ataaagagtt
    28081 cgtaattgcg ctgatctctt atatagctgt tctcattatc tctctaccct gaagtgactc
    28141 tctcacctgt aaaaataata tctcacaggc ttaatagttt cttaatacaa agcctgtaaa
    28201 acgtcaggat aacttcagag gtcgtcggta atttatgatg aacagcacca ataaacttag
    28261 tgttattatt ccgttatata atgcgggcga tgatttccgc acttgtatgg aatctttaat
    28321 tacgcaaacc tagactgctc tggaaatcat tattattaac gatggttcaa cggataattc
    28381 tgttgaaata gcaaagcatt acgcagaaaa ctatccgcac gttcgtttgt tgcatcaggc
    28441 gaatgctggc gcatcggtag cgcgtaatcg tgggatcgag gtggcgacgg gcaaatatgt
    28501 cgcttttgtc gatgctgacg atgaggtcta tcccaccatg tacgaaacgc tgatgaccat
    28561 ggcgttagag gacgacctcg acgtggcgca gtgcaacgct gactggtctt ttcgtgaaac
    28621 gggagaaacc tggcaatcca tccccagcga tcgccttcgc tcaaccggcg tattaaccgg
    28681 cccggactgg ctgcggatgg ggctttcttc gcgccgttgg actcacgttg tctggatggg
    28741 ggtttatcgc cgtgatgtta ttgttaaaaa taacattaaa tttattgccg gattacatca
    28801 tcaggatatt gtctggacaa cagaattcat gtttaacgcg ctgcgtgcgc gatataccga
    28861 gcaatcatta tataaatatt atctgcataa tacgtcagtg agtcggttgc atagacaagg
    28921 gaataaaaac cttaattatc aacgtcacta tattaagatt acccgcctgc tggagaaatt
    28981 aaatcgaaat tatgccgaca aaattacgat ttatccggaa tttcatcagc aaataactta
    29041 cgaagcattg cgtgtttgcc atgcggtgcg caaagagccg gatattctta cccgccaacg
    29101 gatgattgcc gagatattta cttccggtat gtataagcgc ctgattacca atgtgcgcag
    29161 cgtgaaggtc ggttaccagg cgttactgtg gtctttccgc ttatggcaat ggcgcgacaa
    29221 aacgcggtcg caccatcgca ttacgcgtag cgcctttaat ttgcgctagc gttaaacttc
    29281 gccgcttcgg gctgtttgcc ccagccttgc cactgatgct ggaagtaaac gatcagcgtg
    29341 ctctggctga tgctttcgct taatacttca aagaagcgat tagcataaac aggttctatc
    29401 ggtttcttcg gtttgcacag cttcacgcca cggaacggat tacgcggtgc atcaggcaca
    29461 gcgtttttcg gcggtgtaga agtatcaacc tgcggttcat tcagcaagct gctggctttc
    29521 accagcgtaa tgtctggcgg caggttataa accatctgtt gtagcacgcg taccgttaaa
    29581 gggtgaggat gcccaatggc aatggttgaa ccgttgcgac gcgccagatc aattgcgcga
    29641 ttaaattgca cacggatgtc cgcttcgttc tgcgaatcgt cgaggaacac cttccgttta
    29701 ataaccttca cgccagtacc ttgcgcggcg cgcatcgcct gggtattacc gatggttacg
    29761 ctgtcgagga agtaaagatt gtaacgctcc agtgcctgca tcactttctg cataccaaac
    29821 aggttagagg tcatcttgct acccatgtgg ttgttgatcc ccacggcata gggcacgtta
    29881 ttgaccgcac tacgaataat gcgctcaatt tcgtcgctgc tcatctccgg gcgcagcgta
    29941 tttttctcca gcggctgttt actcagtggt gccatcggga gatgaatcaa cacttcgtgc
    30001 ccgctgttat gcgctttggt cgccatctct ctggcgtgcg gtgaatcggg taatacagcg
    30061 acggagatag cggagggcat cgccagcacc tggttttcgt tgtgcgggcg atacccaaaa
    30121 tcatcaatga cgatggcaag tttgccagca agtacggggg aggagagcgc caacagagcg
    30181 gcaaatgcaa gaacgttacg acgaaatgga aacaaaactt atcttcccaa ccacggctgt
    30241 ggattgaccg cctgaccctg gcggcgaatt tcgaaataga gtgaaggccg accctgaccg
    30301 ccactgctgc ccaccagtgc aattggctgg cccgcgcgaa cctgcgaacc aacgctcacc
    30361 agtgcgctct gattatagcc gtaaagactc atgtcgcctt taccgtgctc aaccaccacc
    30421 accagaccgt aaccttgcag ccagtcagcc agaatcactc gaccgtcggc aattgcttta
    30481 acttcagtac cttcagaggc accgataacc atccctttcc agcgtaattc accctgtagc
    30541 tgctcgccat agcgatgcag cgtcggcccg cgaaccggcc agaatgcctg accacgcggc
    30601 gcacccagac caccagttcg ggacatcagc gatttttcgc tttcggtcgg tttgtaggtg
    30661 gtgcctttgc gcgtcgcttc tttctggcgg tcgcgaaccg cctgggcctc acgcgcttct
    30721 cgttcagcac gcgctttcgc cgcggcttcc gcacgggcaa tgctgttacg cagacgggat
    30781 tcgttggcac gcagttcact tagctgctgc tgaccttgct ggatggaaga ttccagcccc
    30841 gccagcgtct ttttacgctc gttcagagcc tgagtcagtt tcgcctgttg ggcgcgctgt
    30901 tcatataaca gcgtttgttg ctcgctttgt ttctcttcca gttcagcacg ctgcatggcg
    30961 acttcttcac gcgtttgctt caactgggca atggtttctt gtcgcgcctg gttgagatag
    31021 ccgaaataag cctgtaaacg ctgtccacac tggctttctt caccgctgag aattagctga
    31081 ataccggtat gctcgccctg acggaatgcg gcatccagtt gtgcggcgag gctgcgctcc
    31141 tgggcggctt tttgctgctc cagtttggca atcgacgcgt tcatctcatc aatctgttta
    31201 ttgagttgat tgagcgtgtt ttgcgtttcg cgcagcttac gggtggcttc agagatcgct
    31261 tcttcctgct ttttcaattg tgcgagcagg cttgcgcgtt gttgttgctt ttggcgtacc
    31321 gcgcgctctt ttgcggcgat atcggcctga atagatttga gttggtcacg ctcatccgcg
    31381 tgggcggaaa aggcgcacaa caatacgcca gcgctaagaa cgctggcgta gatgatgggc
    31441 ctgattgcaa acctgcgcgg tttcacggcc cgtgtcatgg tattaatcgc ctttcccctc
    31501 atggggaggg attattccac gatgaacagc ggcttaccag tcatctcttg cgggatttcc
    31561 atacccatca gcgacaacat ggtcggcgcg atgtcagaaa gtttgccgcc tgcaaccgct
    31621 ttcacgttct tatcaccaac gtaaatcagc ggaactggca ggttggtgtg tgccgtgtgt
    31681 gcctgacccg ttgccggatc gcgcatctgt tcagcgttac cgtggtcagc ggtgatcagc
    31741 agttgtccac ccacggactc aaccgctttc gcgacttctt ccacgcagtg atccagtgct
    31801 tcaaccgctt taaccgccgc ttccatcacc ccggtgtgac ctaccatgtc gccgttcgga
    31861 tagttacaga taatggtgtc gtatttgccg ctcttgatgg ccgcaaccag tttttcggtc
    31921 agctctgcgg agctcatttc cggttgcaga tcgtaggtag ccactttcgg tgagttgatc
    31981 agaatgcgat cttcgccttt gaatgactct tctacgccac cgttgaagaa gaaagtaacg
    32041 tgggcatatt tttcggtttc ggaaatacgc aactgagttt tgtcgttttt cgccatccac
    32101 tcgccgaagg tgttaaccag ggaagcgggt gggtaagcaa ccgcagtttt gatgtcagcg
    32161 gcgtattcgg tcagcatcac gaaatcgacg ttaaccactt tcttacgcgc gaagccgtcg
    32221 aaatctgcgt ttacgaatgc acgagtgatt tcgcgcgcgc ggtcagcacg gaagttcatg
    32281 aaaatcagcg catcgccgtc ttccattgcc gcatctggct gaccttctgc acgaataacg
    32341 gtcgctttca cgaactcgtc gttttcgtcg cgagcataag ctgcctgcaa accagcaacg
    32401 gcagtatcgg cctggaactc accctgcgcc agagtcagca ggtcataggc tttttcaacg
    32461 cgatcccaac ggttatcgcg gtccatcgcg tagtaacgac caatgatgga cgctacgcgg
    32521 cctttaccca gcgcagcaaa tttctcttca aattttttca gcgaggattc agcactgcgc
    32581 ggcggggtgt cgcgaccgtc aaggaatgcg tgcaggtaga ttttttctgc gccgcgttca
    32641 gctgccagtt ctaccatcgc catgatgtga tcttcgtggc tgtgtacgcc gcctgcggag
    32701 agcagaccca taatgtgtac tgctttacct gcgtttttcg ctttatctac cgcgccagtc
    32761 agcactggat tagcaaagaa agcgcgatct ttgatttcaa cgtccagacg agtcaggtcc
    32821 tgatacacga tgcggccggc acccaggtta acgtggccta cttcggagtt acccatctga
    32881 cggtcaggca gaccgacttc cagaccggaa gcgtcgatca gggtatgcgg acgattggcc
    32941 cacagtgcat ccattaccgg ggttttagca ctaaaaatgg cgttatcctg ctgttcttca
    33001 cgatagccat agccatccag aatcaccagt accataggtt ttttagaaac cgacattgcg
    33061 acaacctcat actcaagagt caaaatttgc gtaattttac tacagctgaa tcgataaaat
    33121 agcctctgaa gatcaaaata tacgaccgct cgcggcaagc cgatgccaga tttgcgcttt
    33181 tttttcgtcg tgccccgcaa aattgcattc cagttaacgc gctggctgta tttgccgcac
    33241 cgcgcaggta tactcctttc ctggtttttt taatcactac gtcgggagtt gttacccccc
    33301 atgcaagaaa ttatgcaatt tgttggccgt catcccatac tgagtatcgc ttggatcgcg
    33361 ttactggtgg cggttcttgt gactacgttt aagagcctga cctcgaaagt gaaggtgatt
    33421 actcgtggtg aagctacgcg tctgatcaac aaagaagacg ctgtggttgt ggatttacgt
    33481 cagcgtgatg acttccgtaa aggccatatc gcaggttcta tcaatctgtt gccgagcgaa
    33541 atcaaagcca acaatgttgg tgagcttgag aagcacaaag acaaaccggt tatcgtggta
    33601 gacggttctg gcatgcagtg ccaggagcct gcaaacgcgc tgacgaaagc tggttttgcg
    33661 caagtattcg tgctgaaaga gggcgttgct ggctgggctg gcgaaaactt gcctctggtg
    33721 cgcggcaaat aatttacctg tcgtctttca tgatgcaggc cgttagctgc aattaggccc
    33781 ccagcgactt attgttataa gcttctgggg acactccatt ttgcagccgc cctgcaacat
    33841 gaaatttcag ggtaaaaaaa caggagctaa attatggcca atgttgaaat ctataccaaa
    33901 gaaacctgcc cgtattgcca tcgtgcaaaa gcactgctga gcagcaaggg cgtgagtttt
    33961 caggagttgc cgatcgatgg caatgccgcc aagcgtgaag agatgatcaa acgcagcggt
    34021 cgcaccacgg ttccccagat ttttattgac gcacagcaca ttggcggctg tgatgacttg
    34081 tatgcattgg atgcacgtgg tggactggat cccctgctga aataacgtgt gaacgttggc
    34141 attacattgc gcagtattta aggacaacac ttaagggttt tctacacatg tcagaacaaa
    34201 acaacactga aatgactttc cagatccaac gtatttatac caaggatatc tctttcgaag
    34261 cgccgaacgc gccgcacgtt ttccagaaag attggcaacc agaagttaaa cttgatctgg
    34321 atacggcatc ttcccaactg gcagatgacg tatacgaagt ggtactgcgt gttaccgtaa
    34381 cggcctccct gggcgaagaa accgcgttcc tgtgtgaagt tcagcagggc ggtattttct
    34441 ccatcgcggg tatcgaaggc acccagatgg cacattgcct gggcgcatac tgcccgaaca
    34501 ttctgttccc gtatgctcgt gagtgcatca ccagcatggt atcccgcggt acattcccgc
    34561 aactgaacct tgcgccggtt aacttcgatg cgctgttcat gaactatttg cagcagcagg
    34621 ctggcgaagg tactgaagaa catcaggatg cctgatgaac caacgtaatg cttcaatgac
    34681 tgtgatcggt gccggctcgt acggcaccgc tcttgccatt accctggcaa gaaatggcca
    34741 cgaggttgtc ctctggggcc atgaccctga acatatcgca acgcttgaac gcgaccgctg
    34801 taacgccgcg tttctccccg atgtgccttt tcccgatacg ctccatcttg aaagcgatct
    34861 cgccactgcg ctggcagcca gccgtaatat tctcgtcgtc gtacccagcc atgtctttgg
    34921 tgaagtgctg cgccagatta aaccgctgat gtgtcctgat gcgcgtctgg tgtgggcgac
    34981 caaagggctg gaagcggaaa ccggacgtct gttacaggac gtggcgcgtg aggccttagg
    35041 cgatcaaatt ccgctggcgg ttatctctgg cccaacgttt gcgaaagaac tggcggcagg
    35101 tttaccgacg gctatttcgt tggcctctac cgaccagacc tttgccgatg atctccaaca
    35161 attgctgcac tgtggcaaaa gtttccgcgt ttacagcaac ccggatttca tcggtgtgca
    35221 gcttggcggt gcggtgaaaa acgtcattgc cattggcgcg gggatgtccg acggtatcgg
    35281 ttttggtgcg aatgcgcgta cggcactgat cacccgtggg ctggctgaaa tgtcgcgtct
    35341 tggcgcggcg ctgggtgccg agcctgccac ctttatgggc atggcggggc tgggcgatct
    35401 tgtgcttacc tgtaccgaca accagtcgcg taaccgccgt tttggcatga tgctcggtca
    35461 gggcatggat gtacaaagcg cgcaggagaa gattggtcag gtggtggaag gctaccgcaa
    35521 tacgaaagaa gtccgcgaac tggcgcatcg cttcggcgta gaaatgccaa taaccgagga
    35581 aatttatcaa gtattatatt gcggaaaaaa cgcgcgcgag gcagcattga cgttattagg
    35641 tcgtgcacgc aaggacgagc gcagcagcca ctaaccccag ggaacctttg ttaccgctat
    35701 gacccggccc gcgcagaacg ggccggtcat tatctcatcg tgtggagtaa gtaatgtcgt
    35761 gtgaagaact ggaaattgtc tggaacaata ttaaagccga agccagaacg ctggcggact
    35821 gtgagccaat gctggccagt ttttaccacg cgacgctact caagcacgaa aaccttggta
    35881 gtgcactgag ctacatgctg gcgaacaagc tgtcatcgcc aattatgcct gctattgcta
    35941 tccgtgaagt ggtggaagaa gcctacgccg ctgacccgga aatgatcgcc tctgcggcct
    36001 gtgatattca ggcggtgcgt acccgcgacc cggcagtcga taaatactca accccgttgt
    36061 tatacctgaa gggttttcat gccttgcagg cctatcgcat cggtcactgg ttgtggaatc
    36121 aggggcgtcg cgcattggca atctttctgc aaaaccaggt ttctgtgacg ttccaggtcg
    36181 atattcaccc ggcagcaaaa attggccgtg gcattatgct cgaccacgcc acgggcatcg
    36241 ttgtgggtga aacggcggta attgaaaacg acgtatcgat tctgcaatct gtgacgcttg
    36301 gcggtacggg taaatctggt ggtgaccgtc acccgaaaat tcgtgaaggt gtgatgattg
    36361 gcgcgggcgc gaaaatcctc ggcaatattg aagttggtcg cggcgcgaag attggcgcag
    36421 gttccgtggt gctgcaaccg gtgccgccgc atacaaccgc cgctggcgtt ccggctcgta
    36481 ttgtcggtaa accagacagc gataagccat caatggatat ggaccagcat ttcaacggta
    36541 ttaaccatac atttgagtat ggggatggga tctaatgtcc tgtgatcgtg ccggatgcga
    36601 tgtaatcatc tatccggcat acagtaacta atctctcaac aacgctcccg gatatcccag
    36661 ctgccgccag gcttcataca ccactaccga caccgcattg gacagattca tgctgcggct
    36721 gtccggcacc atcggaatgc gaattttttg ttcagcgggc agggcatcaa gaatgctcgc
    36781 tggcaggccg cgtgtttccg ggccgaacat cagataatcg ccatcctgat agcttacggc
    36841 gctgtgagca ggcgtacctt tcgtggtgag ggcaaacagg cgctggggat tttctgcttc
    36901 gaggaacgcg cgatagtcat gatgacgcgt aacggcggta aactcgtgat agtccagccc
    36961 cgcgcggcgc aggcgcttat cgtcccaggc aaatcccatc ggttcgatga tatgcagacg
    37021 aaagccggta ttagcgcaaa gacggatgat gttgccagta tttggcggaa tttctggttc
    37081 gtaaagtacg atgtttagca tactgccccc ttagtgcggg ggcaggatag cagaaaattg
    37141 gtgaaccgtg cggccttttc ccctcacccc ggccctcccc ctgtctcttg atcaggagat
    37201 ctgatcaaga gacagctccc tgagggagag ggttaggggg gtgcccctat taagttgtca
    37261 agcatgttat gacccctgcg gggtataaaa agtcccgtcg cgcatcatgg cgaacagaa
//
LOCUS       DAJVUJ010000018        36827 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.018, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000018 DAJVUJ010000000
VERSION     DAJVUJ010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36827
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.018"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            584..670
                     /gene="leuL"
                     /locus_tag="OQQ48_000879"
     CDS             584..670
                     /gene="leuL"
                     /locus_tag="OQQ48_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HCS1951733.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            763..2334
                     /gene="leuA"
                     /locus_tag="OQQ48_000880"
     CDS             763..2334
                     /gene="leuA"
                     /locus_tag="OQQ48_000880"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCS1951734.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            2334..3425
                     /gene="leuB"
                     /locus_tag="OQQ48_000881"
     CDS             2334..3425
                     /gene="leuB"
                     /locus_tag="OQQ48_000881"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HCS1951735.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDITGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            3428..4828
                     /gene="leuC"
                     /locus_tag="OQQ48_000882"
     CDS             3428..4828
                     /gene="leuC"
                     /locus_tag="OQQ48_000882"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HCS1951736.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGQVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            4839..5444
                     /gene="leuD"
                     /locus_tag="OQQ48_000883"
     CDS             4839..5444
                     /gene="leuD"
                     /locus_tag="OQQ48_000883"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HCS1951737.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMN"
     gene            complement(5493..6575)
                     /locus_tag="OQQ48_000884"
     CDS             complement(5493..6575)
                     /locus_tag="OQQ48_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1951738.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNTIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNAPLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSIAAGIFTLSLVLIAFML
                     PSVARVELPSENALSMQGGWQDSNVRMLFVASTLMWTCNTMYIIDMPLWISSELGLPD
                     KLAGFLMGTAAGLEIPAMILAGYYVKRYGKRRMMVIAVAAGVLFYTGLIFFHSRMALM
                     TLQLFNAVFIGIVAGIGMLWFQDLMPGRAGAATTLFTNSISTGVILAGVIQGAIAQSW
                     GHFAVYWVIAVISVVALFLTAKVKDV"
     gene            complement(6677..6808)
                     /gene="sgrT"
                     /locus_tag="OQQ48_000885"
     CDS             complement(6677..6808)
                     /gene="sgrT"
                     /locus_tag="OQQ48_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HCS1951739.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            6897..8552
                     /gene="sgrR"
                     /locus_tag="OQQ48_000886"
     CDS             6897..8552
                     /gene="sgrR"
                     /locus_tag="OQQ48_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HCS1951740.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     TTNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVNYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      8586..8685
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8716..9699
                     /gene="thiB"
                     /locus_tag="OQQ48_000887"
     CDS             8716..9699
                     /gene="thiB"
                     /locus_tag="OQQ48_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HCS1951741.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISGWQRAVSR"
     gene            9675..11285
                     /gene="thiP"
                     /locus_tag="OQQ48_000888"
     CDS             9675..11285
                     /gene="thiP"
                     /locus_tag="OQQ48_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HCS1951742.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPDEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLQQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            11269..11967
                     /gene="thiQ"
                     /locus_tag="OQQ48_000889"
     CDS             11269..11967
                     /gene="thiQ"
                     /locus_tag="OQQ48_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HCS1951743.1"
                     /translation="MLKLTDITWLYQHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(12080..12844)
                     /locus_tag="OQQ48_000890"
     CDS             complement(12080..12844)
                     /locus_tag="OQQ48_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCS1951744.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(12930..13808)
                     /gene="araC"
                     /locus_tag="OQQ48_000891"
     CDS             complement(12930..13808)
                     /gene="araC"
                     /locus_tag="OQQ48_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HCS1951745.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            14147..15847
                     /gene="araB"
                     /locus_tag="OQQ48_000892"
     CDS             14147..15847
                     /gene="araB"
                     /locus_tag="OQQ48_000892"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HCS1951746.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLNWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQTQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVPTL
                     "
     gene            15858..17360
                     /gene="araA"
                     /locus_tag="OQQ48_000893"
     CDS             15858..17360
                     /gene="araA"
                     /locus_tag="OQQ48_000893"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HCS1951747.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            17560..18255
                     /gene="araD"
                     /locus_tag="OQQ48_000894"
     CDS             17560..18255
                     /gene="araD"
                     /locus_tag="OQQ48_000894"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCS1951748.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQSGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMEIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            18330..20681
                     /gene="polB"
                     /locus_tag="OQQ48_000895"
     CDS             18330..20681
                     /gene="polB"
                     /locus_tag="OQQ48_000895"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HCS1951749.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGSENGDASSLDFELEYVASRPQLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            20845..23751
                     /gene="rapA"
                     /locus_tag="OQQ48_000896"
     CDS             20845..23751
                     /gene="rapA"
                     /locus_tag="OQQ48_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HCS1951750.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHMPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLLDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            23763..24422
                     /gene="rluA"
                     /locus_tag="OQQ48_000897"
     CDS             23763..24422
                     /gene="rluA"
                     /locus_tag="OQQ48_000897"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HCS1951751.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(24539..25354)
                     /gene="djlA"
                     /locus_tag="OQQ48_000898"
     CDS             complement(24539..25354)
                     /gene="djlA"
                     /locus_tag="OQQ48_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HCS1951752.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            25609..27963
                     /gene="lptD"
                     /locus_tag="OQQ48_000899"
     CDS             25609..27963
                     /gene="lptD"
                     /locus_tag="OQQ48_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HCS1951753.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWQHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVEHFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            28016..29302
                     /gene="surA"
                     /locus_tag="OQQ48_000900"
     CDS             28016..29302
                     /gene="surA"
                     /locus_tag="OQQ48_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HCS1951754.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            29302..30291
                     /gene="pdxA"
                     /locus_tag="OQQ48_000901"
     CDS             29302..30291
                     /gene="pdxA"
                     /locus_tag="OQQ48_000901"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HCS1951755.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTAGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMILNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            30288..31109
                     /gene="rsmA"
                     /locus_tag="OQQ48_000902"
     CDS             30288..31109
                     /gene="rsmA"
                     /locus_tag="OQQ48_000902"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCS1951756.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            31112..31489
                     /gene="apaG"
                     /locus_tag="OQQ48_000903"
     CDS             31112..31489
                     /gene="apaG"
                     /locus_tag="OQQ48_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HCS1951757.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            31496..32338
                     /gene="apaH"
                     /locus_tag="OQQ48_000904"
     CDS             31496..32338
                     /gene="apaH"
                     /locus_tag="OQQ48_000904"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HCS1951758.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(32416..32895)
                     /gene="folA"
                     /locus_tag="OQQ48_000905"
     CDS             complement(32416..32895)
                     /gene="folA"
                     /locus_tag="OQQ48_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HCS1951759.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(33087..34949)
                     /gene="kefC"
                     /locus_tag="OQQ48_000906"
     CDS             complement(33087..34949)
                     /gene="kefC"
                     /locus_tag="OQQ48_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HCS1951760.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLATSSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(34942..35472)
                     /gene="kefF"
                     /locus_tag="OQQ48_000907"
     CDS             complement(34942..35472)
                     /gene="kefF"
                     /locus_tag="OQQ48_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HCS1951761.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGKALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(35580..36827)
                     /locus_tag="OQQ48_000908"
                     /pseudo
     CDS             complement(35580..36827)
                     /locus_tag="OQQ48_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         8959 a        10084 c         9691 g         8093 t
ORIGIN      
        1 catgcatata aaataagaaa aagcaaaatg agtaaaattc ggaaaacata aagatacggg
       61 ttctgataaa aacagaatcc attgccgtgg aatgagtcat ttaccacagc ataataatcc
      121 ataatgtttt tgttgcgaaa acaatctaat cataactacc gcgaatactc aatcatctac
      181 aaaatggatt aaatgtgctt atttaataat taatttatat atttaatgca ttaattatta
      241 acattaatgg atcaataata ttcaccatat caatatcaaa aaaaattcgc aaaacatata
      301 attcaataca aatcatcagg ataggttttg caacgcgtgc attttgtccc ctttttcctc
      361 gttgattaga tgcaaaaatt tattctgaaa tatgtcaacc tatgaaaagt gtcggtcgtt
      421 aaacagaaat taatatcgct tgctttaacc accgcagcac aattagctaa ttttacggat
      481 gcagaactcg cgctggcggg acgtttttat tgcgtcaggg ttgacatccg tttttgtatc
      541 cagtaactct aaaagcatat cgcattcatc tggagctgat ttaatgactc acatcgttcg
      601 ctttatcggt ctactactac taaacgcatc ttctttgcgc ggtagacgag tgagcggcat
      661 ccagcattaa gccagcacgc agtcaaacaa aaaacccgcg ccattgcgcg ggttttttta
      721 tgcccgaagc gaggcgctct aaaagagaca aggacccaaa ccatgagcca gcaagtcatt
      781 attttcgata ccacattgcg cgacggtgaa caggcgttac aggcaagctt gagtgtgaaa
      841 gaaaaactgc aaattgcgct ggcccttgag cgtatgggtg ttgacgtgat ggaagtcggt
      901 ttccccgtct cttcgccggg cgattttgaa tcagtgcaaa ccatcgcccg ccaggttaaa
      961 aacagccgcg tatgcgcgtt agctcgctgc gtggagaaag atatcgacgt ggcggccgaa
     1021 tccctgaaag tcgccgaagc cttccgtatt cataccttta ttgccacttc gccaatgcac
     1081 atcgccacca agctgcgcag cacgctggat gaagtgatcg aacgcgctat ctatatggtg
     1141 aaacgcgccc gtaattacac cgatgatgtt gaattttctt gcgaagatgc cgggcgtacc
     1201 cccattgccg atctggcgcg agtggtcgaa gcggcgatta atgccggtgc caccaccatc
     1261 aacattccgg acaccgtggg ctacaccatg ccgtttgagt tcgccggaat catcagcggt
     1321 ctgtatgaac gcgtacctaa catcgacaaa gccattattt ccgtacatac ccacgacgat
     1381 ttaggcctgg cggtcggcaa ctcactggcg gcggtacatg ccggtgcacg ccaggtggaa
     1441 ggtgcaatga acgggatcgg cgagcgtgcc ggtaactgtt cgctggaaga agtcatcatg
     1501 gcgattaaag ttcgtaagga tattctcaac gtccacaccg ccattaatca ccaggagata
     1561 tggcgcacca gccagttagt tagccagatt tgtaatatgc cgatcccggc aaacaaagcc
     1621 attgttggca gcggcgcatt cgcccactcc tccggtatcc accaggatgg cgtgctgaaa
     1681 aaccgcgaaa actacgaaat catgacacca gaatctattg gtctgaacca aatccagctg
     1741 aatctgacct ctcgttcggg gcgtgcggcg gtgaaacatc gcatggatga gatggggtat
     1801 aaagaaagtg aatataattt agacaatttg tacgatgctt tcctgaagct ggcggacaaa
     1861 aaaggtcagg tgtttgatta cgatctggag gcgctggcct tcatcggtaa gcagcaagaa
     1921 gagccggagc atttccgtct ggattacttc agcgtgcagt ctggctctaa cgatatcgcc
     1981 accgccgccg tcaaactggc ctgtggcgaa gaagtcaaag cagaagccgc caacggtaac
     2041 ggtccggtcg atgccgtcta tcaggcaatt aaccgcatca ctgactataa cgtcgaactg
     2101 gtgaaataca gcctgaccgc caaaggtcac ggtaaagatg cgctgggtca ggtggatatt
     2161 gtcgccaact acaacggtcg ccgcttccac ggcgtcggcc tggccaccga tattgtcgag
     2221 tcctccgcca aagccatggt gcacgtactg aacaatatct ggcgtgccgc agaagtcgaa
     2281 aaagagttgc aacgcaaagc tcaacacaac gaaaacaaca aggaaaccgt gtgatgtcga
     2341 agaattacca tattgccgta ttgccggggg acggtattgg tccggaagtg atgacccagg
     2401 cgctgaaagt gctggatgcc gtgcgcaacc gctttgcgat gcgcatcacc accagccatt
     2461 acgatgtagg cggcgcagcc attgataacc acgggcaacc actgccgcct gcgacggttg
     2521 aaggttgtga gcaagccgat gccgtgctgt ttggctcggt aggcggtccg aaatgggaac
     2581 atttaccacc agaccagcaa ccagaacgcg gcgcgctgtt gcctttgcgt aagcacttca
     2641 aattattcag caacctgcgt ccggcaaaac tgtatcaggg gctggaagca ttctgtccgc
     2701 tgcgtgctga cattgccgct aacggcttcg acatcctgtg cgtgcgcgaa ctgaccggcg
     2761 gcatctattt cggtcagcca aaaggccgcg aaggtagcgg acagtatgaa aaagcgtttg
     2821 ataccgaggt gtatcaccgt tttgagatcg aacgtatcgc ccgcatcgcg tttgaatctg
     2881 cccgcaagcg tcgccacaaa gtcacctcaa tcgacaaagc caacgtgctg caatcctcta
     2941 ttttatggcg ggagatcgtt aacgagatcg ccacggaata cccggatgtc gaactggcgc
     3001 atatgtacat cgacaacgcc accatgcagc tgattaaaga tccatcacag tttgacgtcc
     3061 tgctgtgctc caacctgttt ggcgacattc tgtctgacga gtgcgcaatg atcactggct
     3121 cgatggggat gttgccttcc gccagcctga acgagcaagg ttttggtctg tatgaaccgg
     3181 caggcggctc agcaccagat atcacaggca aaaacatcgc caacccgatt gcgcaaattc
     3241 tgtcgctggc actgctgctg cgctacagcc tggatgccga tgatgcggct tgcgccattg
     3301 aacgcgccat taaccgcgca ttagaagaag gcattcgcac cggggattta gcccgtggcg
     3361 ctgccgccgt tagtaccgat gaaatgggcg atatcattgc ccgctatgtg gcagaagggg
     3421 tgtaatcatg gctaaaacgt tatacgaaaa attattcgac gctcacgtag tgtacgaagc
     3481 cgaaaacgaa accccgctgt tatatatcga ccgccacctg gtgcatgaag tgacctcacc
     3541 gcaggcgttc gatggtctgc gcgcccacgg tcgcccggta cgtcagccgg gcaaaacctt
     3601 cgccactatg gatcacaacg tctctaccca gacgaaagac attaatgcct gtggtgaaat
     3661 ggcgcgcatc cagatgcagg agctgattaa aaactgcaaa gaatttggcg tcgaactgta
     3721 tgacctgaat cacccgtatc aggggatcgt ccacgtaatg gggccggaac agggcgtcac
     3781 cttgccgggg atgaccattg tctgcggcga ctcgcatacc gccacccacg gcgcgtttgg
     3841 cgcactggcc tttggtatcg gcacttccga agttgaacac gtactggcaa cgcaaaccct
     3901 gaaacagggc cgcgcaaaaa ccatgaaaat tgaagtccag ggcaaagccg cgccgggcat
     3961 taccgcaaaa gatatcgtgc tggcaattat cggtaaaacc ggtagcgcag gcggcaccgg
     4021 gcatgtggtg gagttttgcg gcgaagcaat ccgtgattta agcatggaag gtcgtatgac
     4081 cctgtgcaat atggcaatcg aaatgggcgc aaaagccggt ctggttgcac cggacgaaac
     4141 cacctttaac tatgtcaaag gccgtttgca tgcgccgaaa ggcaaagatt tcgacgacgc
     4201 cgttgcctac tggaaaaccc tgcaaaccga cgaaggcgca actttcgata ccgttgtcac
     4261 tctgcaagca gaagaaattt caccgcaggt cacctggggc accaatccag gccaggtgat
     4321 ttccgtgaac gacaatattc ccgatccggc ttcgtttgcc gatccggttg aacgcgcgtc
     4381 ggcagaaaaa gcgctggcct atatggggct gaaaccgggc attccgctga ccgaagtggc
     4441 tatcgacaaa gtgtttatcg gttcctgtac caactcgcgc attgaagatt tacgcgcggc
     4501 agcggagatc gccaaagggc gaaaagtcgc gccaggcgtg caggcactgg tggttcccgg
     4561 ctctggccag gtaaaagccc aggcggaagc ggaaggtctg gataaaatct ttattgaagc
     4621 cggttttgaa tggcgcttgc ctggctgctc aatgtgtctg gcgatgaaca acgaccgtct
     4681 gaatccgggc gaacgttgtg catccaccag caaccgtaac tttgaaggcc gccaggggcg
     4741 cggcgggcgc acgcatctgg tcagcccggc aatggctgcc gctgctgctg tgaccggaca
     4801 tttcgccgac attcgcaaca ttaaataagg agcagaccat ggcagagaaa tttatcaaac
     4861 acacaggcct ggtggttccg ctggatgccg ccaatgtcga taccgatgca atcatcccga
     4921 aacagttttt gcagaaagtg acccgtacgg gttttggcgc gcatctgttt aacgactggc
     4981 gttttctcga tgaaaaaggc caacagccaa acccggactt cgtgctgaac ttcccgcagt
     5041 atcagggcgc ttccattttg ctggcacgag aaaacttcgg ctgtggctcc tcgcgtgagc
     5101 acgcgccctg ggcattgacc gactacggtt ttaaagtggt gattgcgccg agttttgctg
     5161 acatcttcta cggcaatagc tttaacaacc agctgctgcc ggtgaaatta agcgatgcag
     5221 aagtggacga actgtttgcg ctggtgaaag ccaatccggg gatccatttc gacgtggatc
     5281 tggaagcgca agaggtgaaa gcgggagaga aaacctatcg ctttaccatc gatgccttcc
     5341 gccgccactg catgatgaac ggtctggaca gtattgggct taccttgcag cacgacgacg
     5401 ccattgccgc ttatgaagca aaacaacctg cgtttatgaa ttaatcccct tgcccggtca
     5461 aatgaccggg ctttccgcta tcgtccacgt catcaaacgt ctttaacctt tgcggttaaa
     5521 aataatgcga caacagaaat aaccgcaatt acccagtaga cagcaaagtg cccccaactt
     5581 tgtgcaattg ctccctgaat aacgccagcc agaattaccc cggtagaaat actgttagta
     5641 aataaggtgg tagctgcccc cgctcttcca ggcattaaat cctgaaacca tagcatccca
     5701 atacccgcaa caatgccgat aaatacagcg ttaaaaagtt gcagcgtcat caacgccata
     5761 cggctatgaa agaaaatcaa tccggtgtaa aacagtactc ctgccgccac tgctatgacc
     5821 atcattcgcc gcttaccata acgtttgaca tagtagccag ccagaatcat tgctggtatt
     5881 tccagtccag ctgccgtccc catcaggaaa cccgccagtt tgtctggcaa tcctaactcg
     5941 ctactgatcc acaacggcat atcaataatg tacatggtgt tgcaggtcca cattaacgtc
     6001 gaggcgacaa ataacatccg tacgttacta tcctgccagc caccttgcat tgataaagca
     6061 ttttccgacg gcagttctac ccgcgccaca gacggaagca taaatgcaat caataccaga
     6121 ctgagtgtga atatcccggc ggcaatcgaa aacatcacca cttctcgcgc cgagttatcc
     6181 gcatattccc gcgccagagc aaataactgt ggcattgccg tattggccag agatgccaga
     6241 agcacaccac aggtgataag cgtcagataa tgacgattaa atgcaaacaa tggcgcattg
     6301 ccgatagcca tcaaacagca aaatataatc agttttcgcc gatcgccctg actgtcagaa
     6361 cgttttgcca accagaggct tacgccgatc ccagcaatag tattcaccgt ataaaagagt
     6421 ccgatccaga aaggttgcgc gccaacctca cgactcagaa ataagctcaa tgtaggagcc
     6481 tgtagcgccc cggccacccc catcataaaa gcgaccagca taaatgccgc gtaaacaccg
     6541 ttcatacggc gagccatcgt cattatccag atcatacgtt ccctttttag cgcggcgaga
     6601 ataaaaaaaa ccagcaggta taatctgctg gcgggtgatt ttacaccaat actcagtcac
     6661 acatgatgca ggcaagtcaa ctttcagaat tgcggtcatc ccactgcatc agtccttcaa
     6721 gcatggttaa tcgttgtggg acgcttaacc aacgcaacca gcacaacttc gctgtcgcgg
     6781 taaaatagtg ctgataaaac tgacgcatgg ggcacctcct tgcttcatcg ttaggaatta
     6841 ttgacttaat atagggaaaa taaaattgct gtcttttgcg caggagttcc ccttttatgc
     6901 catctgctcg tctgcaacaa cagttcatcc gcctgtggca atgctgcgag ggtaaatcgc
     6961 aggacacaac gctcaacgaa ctggcagcgt tattgagctg ctcgcgtcgt catatgcgta
     7021 ccctgctcaa caccatgcag gatcgcggct ggctgacgtg ggaagcggaa gtcgggcgcg
     7081 gtaaacgctc gcgtctgaca ttcctctata ccgggctggc gcttcagcaa cagcgggcgg
     7141 aagacctgct ggagcaggat cgtatcgacc aactagtgca gttggttggc gacaaagcga
     7201 ctgtgcggca aatgctggtt tctcatctgg gccgcagctt ccgccagggg cggcacatcc
     7261 tgcgcgtgct ctactatcgt ccgttgcgta atctgctacc tggcagcgca ttgcgccgtt
     7321 ccgaaaccca tattgcccgg caaatcttca gttcgctaac gcgcataaat gaggaaaatg
     7381 gggaactgga agcagacatc gcccaccact ggcagcaaat atcaccgctt cactggcgtt
     7441 tctttttgcg tccaggagtc cattttcatc atggtcgtga actggaaatg gacgacgtga
     7501 tcgcctcttt aaaacgaatc aatacgctgc cgctctattc gcatattgcg gacattgtgt
     7561 cgccgacgcc ctggacgctg gatatccatc tcacgcaacc ggaccgctgg ttaccgttac
     7621 tgctggggca agttccggcg atgatcctgc cgcgcgaatg ggaaaccctc agtaactttg
     7681 ccagccatcc catcggcacc ggtccgtatg cggtgatccg caacaccacc aatcaactga
     7741 aaattcaggc attcgatgac ttcttcggtt accgggcatt aatcgatgaa gttaacgtct
     7801 gggttctgcc ggaaattgcc gacgagccag ctggaggtct gatgctaaaa ggtccacagg
     7861 gtgaggaaaa agagattgaa agccgcctgg aggaaggttg ctactattta ctattcgaca
     7921 gccgcaccca tcgcggagcg aatcagcaag tcagggactg ggtaaactat gtgctttctc
     7981 caactaatct ggtctatttc gctgaggaac agtaccagca actgtggttc ccggcttatg
     8041 gactgctccc ccgttggcac catgcccgca ccataaagag cgaaaaaccg gctggcctgg
     8101 aaagcctcac cctgaccttt tatcaggatc acagtgagca tcgggtgatt gccgggatca
     8161 tgcagcagat tctggcaagt caccaggtca cgctggaaat caaagagatc agctacgatc
     8221 agtggcatga aggagagatc gagagcgata tctggcttaa tagcgccaac tttacgctgc
     8281 cgctggattt ttcgctgttc gcgcacttgt gcgaggtgcc gctgctccaa cactgtattc
     8341 cgatcgactg gcaagccgac gccgcccgct ggcgcaatgg cgaaatgaac ctggcgaact
     8401 ggtgccagca actggtcgcc agtaaagcaa tggtgccgct tatccaccac tggctgatca
     8461 ttcagggaca acgcagtatg cgcggcctgc gtatgaatac cctcggctgg ttcgatttta
     8521 aatcagcgtg gtttgcgccg ccggatccat gattgctggt agataacaaa atcactacac
     8581 taacgccgtt ctcaacgggg tgccacgcaa acgcgtgcgc tgagaaaata cccgtcgaac
     8641 ctgatccgga taacgccggc gaagggattt gaggctcctt ctcaagtcct ttgccactct
     8701 tttttgaggt gcaaagtgtt aaaaaaatgt cttcccctgc tgttgctgtg cacagcgccc
     8761 gttttcgcta aacccgttct gactgtttat acctacgatt ccttcgccgc cgactggggg
     8821 cctggtccaa agatcaaaaa agcttttgaa gccgactgta actgcgaact gaaactggtg
     8881 gcgctggaag atggcgtttc gcttctcaac cgtctacgga tggaaggcaa aaatagtaaa
     8941 gccgatgtgg tgctggggct ggataacaat ctgttagacg ccgccagtaa aaccggacta
     9001 tttgccaaaa gcggtgtggc agcggatgcc gttaacgttc ccggcggctg gaataatgac
     9061 actttcgtcc cgtttgatta cggctatttc gccttcgtct atgacaagaa caaactgaaa
     9121 aacccgccgc aaagcctgaa agaattggtt gagagcgatc aaaactggcg ggtgatttat
     9181 caggatccgc gcaccagtac gccggggctg ggcctgctgt tgtggatgca aaaagtctat
     9241 ggcgatgacg ccccacaagc ctggcaaaaa ctggcgaaga aaaccgtcac ggtcaccaaa
     9301 ggctggagcg aagcctacgg cctgttttta aaaggtgaaa gcgatctggt actgagttac
     9361 accacctctc cggcttatca cattctcgaa gagaaaaaag ataactacgc cgccgcgaac
     9421 tttagcgaag gtcactatct gcaggtggaa gtcgccgccc gcaccgctgc cagcaagcag
     9481 ccggagctgg cgcaaaaatt cctccagttt atggtttctc cggcttttca gaatgcgatc
     9541 ccaaccggta actggatgta tccggtggca aatgtcacgc tgcctgccgg gtttgaacaa
     9601 ttgaccaaac cggccaccac gctggagttc acaccagctg aagtggcggc acaacgtcag
     9661 gcatggatta gcggatggca acgcgccgtc agccgttaat tcccggctgg ttaattccag
     9721 gtgtaagcgc cgccacgctg gtggtagcgg ttgcgctcgc ggcgtttctc gccctgtggt
     9781 ggaacgcgcc gcagggtaac tgggtggcag tctggcagga cagctacctg tggcatgtgg
     9841 tgcgcttctc cttctggcag gcgtttctct cggcgctact ctctgtcgta cccgcgatat
     9901 tcctcgcccg cgccctctat cgcaggcgct ttccgggtcg gctggcgctg ttgcgtctgt
     9961 gcgcaatgac cttgatcctc ccggtgctgg ttgctgtttt cggcattctt agcgtctatg
    10021 gtcgccaggg ctggctggca tcgctctgcc aatcgctcgg tctggagtgg accttttcgc
    10081 cctacggcct gcaaggtatt ttgctggcgc acgtattttt taatctgccg atggcgagcc
    10141 gcttattact ccaggcactg gaaaacattc ccgacgaaca acgtcagctt gccgcccagc
    10201 ttgggatgcg cggctggcat tttttccgct tcgtcgaatg gccgtggtta cggcgacaaa
    10261 tcccgccggt tgctgcactt atctttatgc tctgtttcgc cagcttcgcc accgtgctat
    10321 cactgggcgg cggtccgcag gcgaccacta tcgagctggc tatttatcag gcgctgagtt
    10381 acgactacga tcctgcccgc gcggcgatgc tggcgctgat ccagatggtg tgttgccttg
    10441 ggctggtact gttgagtcag cgattgagta aggccattgc gccaggcacc acgctgctgc
    10501 aaggctggcg cgacccggac gatcgtctgc atagccgcat ttgcgacacg gtgttaatag
    10561 tgctggcgtt gctgctgttg ttgccaccgt tgctggcggt gatcgtcgat ggggtaaatc
    10621 gccagttgcc ggaagtgctg gcacaaccgg tgctgtggca ggcgctgtgg acctcgttgc
    10681 gtattgcgct ggcggcaggt gtattgtgcg tagtgctgac catgatgctg ctatggagca
    10741 gtcgcgaact tcgggcacgg cagaaaatgc tggcgggtca ggcgctggag atgagcggca
    10801 tgttgattct cgccatgccg gggattgtgc tggctaccgg cttcttttta ctgctcaaca
    10861 acactatcgg cctgcaacaa tctgctgacg gcattgtgat tttcaccaat gcgttaatgg
    10921 cgatccctta tgcgctgaaa gtactggaaa acccgatgcg cgatatcacc gcccgctaca
    10981 gtatgttgtg tcagtcgctg ggcattgaag gctggtcgcg cttaaaagtg gtggagctgc
    11041 gcgccctgaa acgtccactg gcgcaggcgc tggcctttgc ctgcgtgctg tcgattggtg
    11101 attttggcgt ggtggcgttg ttcggtaacg atgatttccg taccctgccg ttttatctct
    11161 accagcaaat tggctcctat cgcagccagg acggcgcggt caccgcgtta attctgctgc
    11221 tactctgttt tctgctgttt accgtgattg aaaaactacc ggggcgaaat gttaaaactg
    11281 actgatatca cctggcttta ccaacatttg ccgatgcgtt ttagcttaac ggtggaacgc
    11341 ggcgagcagg tggcgatcct cgggccaagc ggcgcaggta aaagtaccct gctgaatttg
    11401 attgccggtt ttctgacgcc agccagcggt tcgctgacta tcgatggcgt tgatcacacc
    11461 acgacgccgc cgtcacgccg tccggtgtcg atgctgtttc aggagaacaa cctgttcagc
    11521 cacctgacgg tcgcgcagaa catcgggctg gggcttaatc caggattgaa gctgaacgcg
    11581 gcacagcagg agaaaatgca cgctatcgcc cgtcagatgg ggattgataa tttaatggcg
    11641 cggttaccgg gcgagctttc cggcggccag cgacagcgcg tggcgttagc gcgttgtctg
    11701 gtgcgcgaac agccgatttt attgctcgat gaaccgttct ctgcgctcga tccggcgtta
    11761 cgtcaggaga tgctgacgct ggtgagcacg agctgccagc agcaaaaaat gacgctattg
    11821 atggtgtcgc acagtgtgga agatgcggcg cggatcgcca cacgctcggt ggtggtcgcc
    11881 gacgggcgca tcgcctggca gggtaagact gaagaattgt tgagcggtaa ggcgagtgct
    11941 tcggcaatat tggggattac gggttagtat gcactgtcag atgcggcgtg aacaccttat
    12001 ccgacctacg gacagtgcgt tttgtaggcc tgataagacc ggtcagcgtc gcatcaggca
    12061 acccgcacaa gacaatcccc taaaccccaa ccactttacg caaaatatcg atatacaccg
    12121 gcatcaacgg gtggcgaatt aacaccacca gcgccaccac gccgatggca gaaatcaacg
    12181 gcgtcagcca caacaaacgg ccgcgcgaca aataatgact taaacgatcg gttgctttac
    12241 cgctgcgcca taaccgccag cacagccagc caccaaccca caaaaatacc gccgtcgcca
    12301 gcagcaacca tttaaactcg ccgctctgca ttccggctgg aatatcgatc gccgcgcccg
    12361 ccagaatccc aggcaggaag taaaacggtg gccacagcag gcagccgata atattcggcg
    12421 taatgaattt tgccaccggc agatccagca ttcccgccac cattggcacc agcggacgcg
    12481 tcgggccaac aaagcgaccg accagaatgg tgaacatgct gtgttgatgc aacgcatgtt
    12541 cagttttatc gagtaatgct ttgtttttct tcagaaatga ccagcgatgc aacggctttt
    12601 taaaacgcca gcccagccag aaggagatcc agtcgcccag cagacaaccg acaatccctg
    12661 ccagccaggc gtgccagaaa cttaactcgc cgctgccaat cagcgctccc agccccgcca
    12721 tcagcaccgt accgggtaga atcaaaccga ccagcgccag cgactccaga aaggccacca
    12781 acaccaccgc catcaatgaa tacacggtag attgggtaat aaagtgttcc agcaatgctt
    12841 gcataatgtg cctgtcaaat ggacgaagca gggattctgc aaaccctatg ctactccgtc
    12901 aagccgtcaa ttgtctgatt cgttaccaat tatgacaact tgacggctac atcattcact
    12961 ttttcttcac aaccggcacg gaactcgctc gggctggccc cggtgcattt tttaaatacc
    13021 cgcgagaaat agagttgatc gtcaaaacca acattgcgac cgacggtggc gataggcatc
    13081 cgggtggtgc tcaaaagcag cttcgcctgg ctgatacgtt ggtcctcgcg ccagcttaag
    13141 acgctaatcc ctaactgctg gcggaaaaga tgtgacagac gcgacggcga caagcaaaca
    13201 tgctgtgcga cgctggcgat atcaaaattg ctgtctgcca ggtgatcgct gatgtactga
    13261 caagcctcgc gtacccgatt atccatcggt ggatggagcg actcgttaat cgcttccatg
    13321 cgccgcagta acaattgctc aagcagattt atcgccagca gctccgaata gcgcccttcc
    13381 ccttgcccgg cgttaatgat ttgcccaaac aggtcgctga aatgcggctg gtgcgcttca
    13441 tccgggcgaa agaaacccgt attggcaaat attgacggcc agttaagcca ttcatgccag
    13501 taggcgcgcg gacgaaagta aacccactgg tgataccatt cgcgagcctc cggatgacga
    13561 ccgtagtgat gaatctctcc tggcgggaac agcaaaatat cacccggtcg gcaaacaaat
    13621 tctcgtccct gatttttcac caccccctga ccgcgaatgg tgagattgag aatataacct
    13681 ttcattccca gcggtcggtc gataaaaaaa tcgagataac cgttggcctc aatcggcgtt
    13741 aaacccgcca ccagatgggc attaaacgag tatcccggca gcaggggatc attttgcgct
    13801 tcagccatac ttttcatact cccgccattc agagaagaaa ccaattgtcc atattgcatc
    13861 agacattgcc gtcactgcgt cttttactgg ctcttctcgc taacccaacc ggtaaccccg
    13921 cttattaaaa gcattctgta acaaagcggg accaaagcca tgacaaaaac gcgtaacaaa
    13981 agtgtctata atcacggcag aaaagtccac attgattatt tgcacggcgt cacactttgc
    14041 tattccatag catttttatc cataagatta gcggatccta cctgacgctt tttatcgcaa
    14101 ctctctactg tttctccata cccgtttttt tggatggagt gaaacgatgg cgattgcaat
    14161 tggcctcgat tttggcagtg attctgtgcg agctttggcg gtggactgcg ccagcggtga
    14221 agagatcgcc accagcgtag agtggtatcc ccgttggcaa aaagggcaat tttgtgatgc
    14281 cccgaataac cagttccgtc atcatccgcg tgactacatt gagtcaatgg aagcggcact
    14341 gaaaaccgtg cttgcagagc ttagcgtcga acagcgcgca gctgtggtcg ggattggcgt
    14401 tgacagtacc ggctcgacgc ccgcaccgat tgatgccgac ggtaacgtgc tggcgctgcg
    14461 cccggagttt gccgaaaacc caaacgcgat gttcgtattg tggaaagacc acactgcggt
    14521 tgaagaagcg gaagagatta cccgtttgtg ccacgcgccg ggcaacgttg actactcccg
    14581 ctacattggt ggtatttatt ccagcgaatg gttctgggca aaaatcctgc atgtgactcg
    14641 ccaggacagc gccgtggcgc aatctgccgc atcgtggatt gagctgtgcg actgggtgcc
    14701 agctctgctt tccggtacca cccgcccgca ggatattcgt cgcggacgtt gcagcgccgg
    14761 gcataaatct ctgtggcacg aaagctgggg cggcttgccg ccagccagtt tctttgatga
    14821 gctggacccg atcctcaatc gccatttgcc ttccccgctg ttcactgaca cctggactgc
    14881 cgatattccg gtgggcacct tatgcccgga atgggcgcag cgtctcggcc tgcctgaaag
    14941 cgtggtgatt tccggcggcg cgtttgactg ccatatgggc gcagttggcg caggcgcaca
    15001 gcctaacgca ctggtaaaag ttatcggtac ttccacctgc gacattctga ttgccgacaa
    15061 acagagcgtt ggcgagcggg cagttaaagg tatttgcggt caggttgatg gcagcgtggt
    15121 acctggattt atcggtctgg aagcaggcca atcggcgttt ggtgatatct acgcctggtt
    15181 tggtcgcgta ctcggctggc cgctggaaca gcttgccgcc cagcatccgg aactgaaagc
    15241 gcaaatcaac gccagccaga aacaactgct tccggcgctg accgaagcat gggccaaaaa
    15301 tccgtctctg gatcacctgc cggtggtgct caactggttt aacggccgcc gcacaccgaa
    15361 cgctaaccaa cgcctgaaag gggtgattac cgatcttaac ctcgctaccg acgctccgct
    15421 gctgtttggc ggtttgattg ctgccaccgc ctttggcgca cgcgcaatca tggagtgctt
    15481 taccgatcag gggatcgccg tcaataacgt gatggcgctg ggcggcatcg cgcggaaaaa
    15541 ccaggtcatt atgcaggcct gctgcgacgt gctgaatcgc ccgctgcaaa ttgttgcctc
    15601 tgaccagtgc tgtgcgctcg gtgcggcgat ttttgctgcc gtcgccgcga aagtgcacgc
    15661 agacatccca tcagcccagc aaaaaatggc cagtgcggta gagaaaaccc tgcaaccgcg
    15721 cagcgaacag acacaacgct ttgaacagct ttatcgccgc tatcagcaat gggcgatgag
    15781 cgccgaacaa cactatcttc caacttccgc cccggcacag gctgcccagg ccgttccgac
    15841 tctataagga cacgataatg acgatttttg ataattatga agtgtggttt gtcattggca
    15901 gccagcatct gtatggcccg gaaaccctgc gtcaggtcac ccaacatgcc gagcacgttg
    15961 ttaatgcgct gaatacggaa gcgaaactgc cctgcaaact ggtgttgaaa ccgctgggca
    16021 ccacgccgga tgaaatcacc gctatttgcc gcgacgcgaa ttacgacgat cgttgcgctg
    16081 gtctggtggt gtggctgcac accttctccc cggccaaaat gtggatcaac ggcctgacca
    16141 tgctcaacaa accgttgctg caattccaca cccagtttaa cgcggcgctg ccgtgggaca
    16201 gtatcgatat ggactttatg aacctgaacc agactgcaca tggcggtcgc gagttcggct
    16261 tcattggcgc gcgtatgcgt cagcaacatg ccgtggttac cggtcactgg caggataaac
    16321 aagcccatga gcgtatcggc tcctggatgc gtcaggcggt ctctaaacag gatacccgtc
    16381 atctgaaagt ctgccgtttt ggcgataaca tgcgtgaagt agcggtcacc gatggtgata
    16441 aagttgccgc acagatcaag ttcggtttct ccgtcaatac ctgggcggtt ggcgatctgg
    16501 tgcaggtggt gaactccatc agcgacggcg atgttaacgc gctggtcgat gagtacgaaa
    16561 gctgctacac catgacgcct gccacacaaa tccacggcga aaaacgacag aacgtgctgg
    16621 aagcggcgcg tattgagctg gggatgaagc gtttcctgga acaaggtggc ttccacgcgt
    16681 tcaccaccac ctttgaagat ttgcacggtc tgaaacagct tccaggtctg gccgtacagc
    16741 gtctgatgca gcagggttac ggctttgcgg gcgaaggcga ctggaaaacc gccgccctgc
    16801 ttcgcatcat gaaggtgatg tcaaccggtc tgcagggcgg cacctccttt atggaggact
    16861 acacctatca cttcgagaaa ggtaatgacc tggtgctcgg ctcccatatg ctggaagtct
    16921 gcccgtcgat tgccgtagaa gagaaaccga tcctcgacgt tcagcatctc ggtattggtg
    16981 gtaaggacga tcctgcccga ctgatcttca atacccaaac cggtccagcg attgtcgcca
    17041 gcctgattga tctcggcgat cgttaccgtc tgctggttaa ctgtatcgac acggtgaaaa
    17101 caccgcactc cctgccgaaa ctgccggtgg cgaatgcgct gtggaaagcg caaccggatc
    17161 tgccaactgc ttccgaagcg tggatcctcg ctggtggcgc gcaccatacc gtcttcagcc
    17221 atgcgctgaa cctcaacgat atgcgtcagt tcgccgagat gcacgacatt gaaatcacgg
    17281 tgattgataa cgacacccgc ctgccagcgt ttaaagacgc actgcgctgg aacgaagtgt
    17341 attacggatt tcgtcgctaa gtagccacat caggtatgta acgcctgatg cgacgctgac
    17401 gcgtcttatc tggcctacac gctgcgattt tgtaggccgg ataagcaaag cgcatccggc
    17461 attcaacgcc tgatgcgacg ctggcgcgtc ttatcaggcc tacgcgctgc gattttgtag
    17521 accggataag cgaagcgcat ccggcacgaa ggagtcaaca tgttagaaga tctcaaacgc
    17581 caggtattag aagccaacct ggcgctgcca aaacacaacc tggtcacgct cacatggggc
    17641 aacgtcagcg ccgttgatcg cgagcgcggc gtctttgtga tcaaaccttc cggcgtcgat
    17701 tacagcgtca tgaccgctga cgatatggtc gtggttagca tcgaaacagg tgaagtggtt
    17761 gaaggtacga aaaagccctc ctccgacacg ccaactcacc ggctgctcta tcaggcattc
    17821 ccctccattg gcggcattgt gcatacgcac tcgcgccacg ccaccatctg ggcgcagtcg
    17881 ggtcagtcga ttccagcaac cggcaccacc cacgccgact atttctacgg caccattccc
    17941 tgcacccgca aaatgaccga cgcagaaatc aacggcgaat atgagtggga aaccggtaac
    18001 gtcatcgtag aaacctttga aaaacagggt atcgatgcag cgcaaatgcc cggcgttctg
    18061 gtccattccc acggcccgtt tgcatggggc aaaaatgccg aagatgcggt gcataacgcc
    18121 atcgtgctgg aagaggtcgc ttatatggag atattctgcc gtcagttagc gccgcagtta
    18181 ccggatatgc agcaaacgct gctggataaa cactatctgc gtaagcatgg cgcgaaggca
    18241 tattacgggc agtaatgact gtataaaacc acagccaatc aaacgaaacc aggctatact
    18301 caagcctggt tttttgatgg attttcagcg tggcgcaggc aggttttatc ttaacccgac
    18361 actggcggga caccccgcaa gggacagaag tctccttctg gctggcgacg gacaacgggc
    18421 cgttgcaggt tacgcttgca ccgcaagagt ccgtggcgtt tattcccgcc gatcaggttc
    18481 cccgcgctca gcatattttg cagggtgaac aaggctttcg cctgacaccg ctggcgttaa
    18541 aggattttca ccgccagccg gtgtatggcc tttactgtcg cgcccatcgc caattgatga
    18601 attacgaaaa gcgcctgcgt gaaggtggcg ttaccgtcta cgaggccgat gtgcgcccgc
    18661 cagaacgcta tctgatggag cggtttatca cctcaccggt gtgggtcgag ggtgatatgc
    18721 acaatggcac tatcgttaat gcccgtctga aaccgcatcc cgactatcgt ccgccgctca
    18781 agtgggtttc tatagatatt gaaaccaccc gccacggtga gctgtactgc atcggcctgg
    18841 aaggctgcgg gcagcgcatc gtttatatgc tggggtcgga gaatggcgac gcctcctcgc
    18901 ttgatttcga actggaatac gtcgccagcc gcccgcagtt gctggaaaaa ctcaacgcct
    18961 ggtttgccaa ctacgatcct gatgtgatca tcggttggaa cgtggtgcag ttcgatctgc
    19021 gaatgctgca aaaacatgcc gagcgttacc gtcttccgct gcgtcttggg cgcgataata
    19081 gcgagctgga gtggcgcgag cacggcttta aaaacggcgt cttttttgcc caggctaaag
    19141 gtcggctaat tatcgacggt atcgaggcgc tgaaatccgc gttctggaat ttctcttcat
    19201 tctcgctgga aactgtcgct caggagctat taggcgaagg aaaatctatc gataacccgt
    19261 gggatcgaat ggacgaaatt gaccgccgtt tcgccgaaga taaacctgcg ctggcaactt
    19321 ataacctgaa agattgcgag ctggtgacgc agatcttcca caaaactgaa atcatgccat
    19381 ttttactcga acgggcaacg gtgaacggcc tgccggtgga ccgacacggc ggttcggtgg
    19441 cggcatttgg tcatctctat tttccgcgaa tgcatcgcgc tggttatgtc gcgcctaatc
    19501 tcggcgaagt gccgccgcac gccagccctg gcggctacgt gatggattca cggccagggc
    19561 tttatgattc agtgctggtg ctggactata aaagcctgta cccgtcgatc atccgcacct
    19621 ttctgattga tcccgtcggg ctggtggaag gcatggcgca gcctgatcca gagcacagta
    19681 ccgaaggttt tctcgatgcc tggttctcgc gagaaaaaca ttgcctgccg gagattgtga
    19741 ctaacatctg gcacgggcgc gatgaagcca aacgccaggg taacaaaccg ctgtcgcagg
    19801 cgctgaaaat catcatgaat gccttttatg gcgtgctcgg caccaccgcc tgccgcttct
    19861 tcgatccgcg gctggcatcg tcgatcacca tgcgtggtca tcagatcatg cggcaaacca
    19921 aagcgttgat tgaagcacag ggctacgacg ttatctacgg cgataccgac tcaacgtttg
    19981 tctggctgaa aggcgcacat tcggaagaag aagcggcgaa aatcggtcgt gcactggtgc
    20041 agcacgttaa cgcctggtgg gcggaaacgc tgcaaaaaca acggctgacc agcgcattag
    20101 aactggagta tgaaacccat ttctgccgtt ttctgatgcc aaccattcgc ggagccgata
    20161 ccggcagtaa aaagcgctac gccgggctga ttcaggaggg cgataaacag cggatggtgt
    20221 ttaaagggct ggaaaccgtg cgcaccgact ggacgccgct ggcccagcag tttcagcagg
    20281 agctatacct gcgcatcttc cgcaacgagc catatcagga atatgtacgc gaaaccatcg
    20341 acaaactgat ggcgggtgaa ctggatgcgc gactggttta ccgtaaacgc cttcgccgtc
    20401 cgctgagcga gtatcagcgt aatgtgccgc ctcatgtacg cgccgctcgc cttgccgatg
    20461 aagaaaacca aaagcgtggt cgccccttgc aatatcagaa tcgcggcacc attaagtacg
    20521 tatggaccac caacggcccg gagccgctgg actaccaacg ttcaccactg gattacgaac
    20581 actatctgac ccgccagcta caacccgtgg cggagggaat actccctttt attgaggata
    20641 attttgctac acttatgacc gggcaacttg ggctattttg agcaaaaaaa gagttcgcca
    20701 gataccattt tgatgcgtga cgaatgcttt gccatccagt accatagcgc cctttccatt
    20761 cctggacctg aataacacca ctacctcata agcacggtag cgggtggtta ttgcctgcaa
    20821 ttaaagatat agagccgaac acatatgcct tttacacttg gtcaacgctg gatcagcgat
    20881 acagaaagcg aattgggact tggaaccgtt gtcgcggtgg atgcgcgaac tgtcacttta
    20941 cttttcccat ctactggtga aaaccgtctg tacgcacgca gtgattcccc cgtgacccgc
    21001 gtgatgttca accctggtga taccattacc agccatgacg gctggcagat gcaagtcgaa
    21061 gaagtaaaag aagaaaatgg cttgctgacc tatatcggta ctcgcctgga tactgaagag
    21121 tccggcgtag ccctgcgtga agttttcctt gatagcaaac tggtgttcag caaaccgcag
    21181 gaccgcctgt ttgccgggca gattgaccgt atggaccgct ttgcgctgcg ttatcgcgcg
    21241 cgtaagtatt ccagcgaaca gttccgtatg ccgtacagcg gcctgcgcgg tcagcgtacc
    21301 agcctgatcc cgcaccagct caacatcgct catgatgttg gccgccgcca cgcgccgcgc
    21361 gtcctgctgg ctgacgaagt gggtttaggg aaaaccattg aagccgggat gatcctgcat
    21421 cagcaactgc tctctggcgc tgctgaacgt gtgctgatta tcgtcccgga aaccttacag
    21481 catcagtggc tggtagaaat gctgcgccgt ttcaacctgc gctttgctct gtttgatgat
    21541 gagcgttatg ccgaagctca gcacgatgct tacaacccgt tcgacaccga acagctggtg
    21601 atttgctcgc tggattttgc ccgtcgtagc aaacagcgcc tggaacatct ctgtgaagcc
    21661 gaatgggacc tgctggtggt cgatgaagcg catcacctgg tgtggagcga agatgcgcca
    21721 agccgtgaat atcaggccat tgaacaactg gcagagcaca tgccgggcgt tctgctgctg
    21781 accgcgaccc cggaacagct ggggatggaa agccacttcg cccgtctgcg tctgctggac
    21841 ccgaaccgtt tccacgattt tgcgcagttc gttgaagagc agaaaaatta tcgtccggtt
    21901 gcggacgccg ttgccatgct gctggcaggt aacaaactga gcaatgacga actgaacatg
    21961 ctcggcgaga tgatcggcga gcaggatatc gagccgctgt tgcaggcagc aaacagcgac
    22021 agcgaagatg cccagagcgc ccgtcaggag ctggtttcga tgctgatgga tcgccacggc
    22081 accagccgcg tgctgttccg taacacgcgt aacggtgtga aaggattccc gaaacgcgag
    22141 ctgcacacca ttaagctgcc gctaccgacg cagtatcaga cggctattaa agtctccggc
    22201 attatgggcg cacgtaaaag tgcggaagat cgtgctcgcg atatgctcta cccggagcgt
    22261 atttatcagg aatttgaagg tgataacgcc acctggtgga acttcgatcc gcgcgttgag
    22321 tggctgatgg gctacctgac cagccatcgc tctcagaaag tgctggtgat ctgcgccaaa
    22381 gctgccactg cgctgcaact ggagcaggta ctgcgcgaac gtgaaggtat tcgcgctgcg
    22441 gtgttccacg aaggtatgtc gattatcgaa cgtgaccgcg ctgccgcctg gtttgccgaa
    22501 gaagacaccg gcgcacaggt actgctgtgc tcagaaatcg gttctgaagg acgtaacttc
    22561 cagttcgcca gccacatggt gatgtttgac ctgccattca acccggatct actggagcag
    22621 cgtattggtc gtctggatcg tatcggccag gcgcacgata ttcagatcca tgtgccttat
    22681 ctggagaaaa ccgctcagtc ggtgctggtg cgctggtatc acgaaggtct ggatgcattt
    22741 gagcacacct gcccgaccgg acgcactatt tacgatagcg tatacaacga tctgattaac
    22801 tatctggctt caccggatca aaccgaaggc tttgacgatc tgatcaaaaa ctgccgcgag
    22861 caacatgaag cgctgaaagc acagctggaa cagggtcgtg accgcctgct ggaaatccac
    22921 tccaacggtg gcgaaaaagc ccaggcactg gcagaaagca ttgaagagca ggatgacgat
    22981 accaacctga tcgccttcgc catgaacctg ctcgatatta tcggtatcaa tcaggacgat
    23041 cgcggcgaca acatgatcgt gctgacgccg tccgatcata tgctggtgcc ggacttccct
    23101 ggcctgtcgg aagatggcat caccatcacc tttgatcgtg aagtggcgct ggcgcgtgaa
    23161 gatgcacagt ttattacctg ggagcatccg ctgatccgca acggtctgga tctgatcctt
    23221 tctggcgata ccggtagcag cacgatttca ctgttaaaaa acaaagcgtt gccggtaggt
    23281 acgctgttgg tggaactgat ttatgtggtt gaagcccagg ctccgaagca gttgcagctc
    23341 aaccgcttcc tgccaccgac gccggtacgt atgctgctgg ataaaaacgg caacaacctg
    23401 gcggcgcagg tagagtttga aacctttaac cgccagctta acgcggttaa ccgtcacacc
    23461 ggcagcaaac tggttaacgc cgtgcagcag gatgttcacg ctatccttca actgggtgaa
    23521 gcgcagatcg agaaatctgc ccgtgcattg attgatgcag cgcgtaacga agccgacgaa
    23581 aaactgtctg ccgagctgtc tcgtctggaa gctctgcgtg cagtgaaccc gaacattcgt
    23641 gacgacgaac tgaccgccat tgagagcaac cgtcagcagg taatggaaag cctggatcag
    23701 gcaggttggc gtctggatgc cctgcgtttg atcgttgtaa cgcatcagta acggagccga
    23761 aaatggggat ggaaaactac aatccaccgc aggaaccctg gttggttatc ctgtatcagg
    23821 atgaccacat tatggtggtt aacaagccga gcggtttgtt gtctgtgccg gggcgtctgg
    23881 aagagcacaa agacagcgtg atgacgcgca ttcagcgtga ttatccgcag gcagaatcgg
    23941 tgcatcgtct ggatatggct accagcggcg tgattgtagt ggcgctgacc aaagccgcgg
    24001 agcgggagtt aaaacgccag ttccgcgagc gcgagccgaa aaagcagtat gtggcccgcg
    24061 tctgggggca tccatcccct gcggaaggtc tggtggatct gccgctgatt tgcgactggc
    24121 caaaccgccc gaaacagaaa gtctgttacg aaacgggtaa acctgcgcag acggaatatg
    24181 aagtggtgga gtatgcggcg gataacacgg caagagtggt gttaaaaccg attactgggc
    24241 gttcgcatca attgcgtgtg catatgctgg cgctggggca tccgattctc ggcgatcgtt
    24301 tttatgcatc accagaagcg agagcgatgg caccgcgttt gttgctacat gcagagatgt
    24361 tgacgattac ccatccggcg tatggcaata gtatgacgtt taaagcgcca gcggattttt
    24421 aatagtcacg ctggcgcagt tcggtaggcg gataaggcgt tcacgccgaa tccggcaatt
    24481 gtgcgccgat tgcctgatgc gacgcgggcc gcgtcttatc aggcctacag tttaaaactt
    24541 atttaaaccc tttctgctgc tttatcagct catatgcctg ctgaatttcc tgcgctttct
    24601 gcttcgccat ctccatcatc tcaggcggca aacctttcgc caccagctta tcgggatggt
    24661 gttcactcat cagcttacgg taggctcgtt taatggtggt cgcatcatcc gtcggcttca
    24721 cacccagcac attacaggca tcttccagcg tcgggccacg ctgcgcttgc tgccagttac
    24781 caccgccaga ttgctgctga taaccgccgc caaactgtgc accgccctgc atcatgcgca
    24841 aaaactggtc aaactgggcg cgggagatcc ctaattcttc tgcaatgaca tacagcaccg
    24901 cccgttcatt cgggtgcagt gaaccatcag caaacgccgc ctgaatctgg atctccagaa
    24961 acatacgaat taagtcaaaa cgaccaaagc agacactgcg aaactggcgc atcttttcgc
    25021 gcagcgggta attgtctgat tttcccaccc ggaacgcgtt ttgcgccgca gtacgggaag
    25081 cgccatgaag gttcattcgg tccatcagct ggctggcgat atgaatatca gcctccgtga
    25141 cgcgaccttt ggatttggtt aaatgcccca tcacttcaaa agtggtggca aaaaacagcg
    25201 cctgacgctc acgctggttg gcgaaccacg ccattttacg gctacgggct ttatcaaaca
    25261 tatggccaat taacaggcct aacactacgc cccaaaagcc gccgcccatc agtaaggcca
    25321 cggccacgcc aatgattttt ccccaatact gcatatattc cccaaatcga cacacggata
    25381 tcagggctat ctcccacaat ataaaggtgc ttttaccgtt ttccggctgc agtcaattgt
    25441 gggacatcgc ctataatttg cattatcata cctgtcattc aatgccgtgc ctaacaccac
    25501 tgacgctatt cggacaggat taacactagc gtagagatga cgagtacgtt agtctctgac
    25561 cgtttgtcac gcgcaacgtt accgatgatg gaacaataaa atcaacgtat gaaaaaacgt
    25621 atccccactc tcctggccac catgattgcc accgcccttt atagtcaaca gggactggca
    25681 gccgacctcg cctcacagtg catgttgggc gtgccaagct atgaccgtcc tctggtacag
    25741 ggcgatacca atgacttacc cgtgactatc aatgctgacc acgcgaaagg ggactacccg
    25801 gatgacgccg tgtttactgg cagcgtggat atcatgcagg gtaacagccg tctgcaggcc
    25861 gacgaagtgc agctccatca aaaagaggca ccaggacaac cggagccggt acgaaccgtt
    25921 gatgcgctcg gtaatgtcca ttacgacgat aaccaggtga tcctcaaagg gccgaaaggc
    25981 tgggcgaatc tgaacaccaa agataccaac gtctgggaag gtgattacca gatggtgggt
    26041 cgccagggcc gcggtaaagc ggacctgatg aaacaacgtg gtgaaaaccg ctataccatt
    26101 ctggataacg gtagctttac ctcctgtctg ccgggttctg acacctggag cgtggtaggt
    26161 agcgaaatta ttcatgaccg cgaagaacaa gttgcggaga tctggaacgc ccgctttaag
    26221 gtgggtccag taccgatctt ttatagcccc tatttgcagt tgccggtggg tgacaaacgt
    26281 cgctctggtt tcttgatccc gaacgccaag tacaccacca ccaactactt tgagttctac
    26341 ctgccatatt actggaacat cgcgccaaat atggatgcca ccatcacgcc gcattatatg
    26401 catcgtcgtg gcaacatcat gtgggagaac gaattccgct acctctccca ggcgggcgct
    26461 ggcttgatgg aactggacta tctgccttca gataaagtct atgaagatga acacccgaac
    26521 gatgacagtt cacgtcgttg gttgttctac tggcaacatt ccggggtcat ggatcaagtg
    26581 tggcgtttca acgtagacta caccaaggtc agcgatccta gctacttcaa tgatttcgat
    26641 aacaagtacg gttccagtac tgacggctac gcaacgcaaa aattcagcgt tggctatgcg
    26701 gtgcaaaact tcaatgccac cgtttcaacc aagcagttcc aggtctttag cgagcagaac
    26761 accagtagct actcggcaga gccgcagtta gacgttaact actaccagaa tgatgttggt
    26821 ccgtttgata cgcgtattta cggccaggca gtgcactttg ttaacaccag agacgacatg
    26881 cctgaagcaa cccgtgttca cctggaaccg accatcaatt tgccgctctc taataactgg
    26941 ggcagcatca ataccgaagc gaagttgctg gcaacccatt atcagcaaac caatcttgac
    27001 tggtataact ccagaaacac gaccaagctg gacgaatccg ttaaccgcgt tatgccgcaa
    27061 ttcaaagttg acggcaaaat ggtctttgaa cgcgatatgg aaatgctggc tccgggttat
    27121 acccaaacgc tggaaccgcg cgcgcagtat ttgtacgtgc cgtatcgcga tcagagcgac
    27181 atctataact acgactcgtc tctactgcaa tctgactact ctggcctgtt ccgggaccgg
    27241 acttacggcg gtcttgaccg tattgcctcc gctaaccagg tgacgaccgg tgtcacatct
    27301 cgcatatatg atgatgctgc cgttgaacat tttaatattt ccgttggtca aatctactat
    27361 ttcacggagt ctcgcactgg cgatgacaac ataacatggg agaatgacga caaaacgggc
    27421 tcactggtgt gggcaggcga tacttactgg cgtatctccg agcgttgggg attgcgtggc
    27481 gggattcagt acgatacacg tctggataac gtagcgacca gtaactccag cattgaatac
    27541 cgtcgggatg aagaccgtct ggtacagctg aattaccgtt acgccagccc ggaatatatt
    27601 caggctacgc tgcctaagta ctattccact gctgagcaat ataagaatgg tatttcgcag
    27661 gtaggtgctg tcgccagctg gccaattgcc gatcgttggt ccattgttgg ggcctactac
    27721 tacgacacca atgcgaacaa gcaagccgac tctatgttag gtgtgcaata cagctcctgc
    27781 tgctatgcaa ttcgcgtcgg ttacgagcgg aagctgaacg gttgggataa cgataaacaa
    27841 catgcggtat atgacaacgc aatcggcttt aacatcgaac ttcgcggcct gagctccaac
    27901 tacggtctgg gtacgcaaga gatgctgcgt tcgaacattc tgccgtatca aaacactttg
    27961 tgatttgttg atttaccacg taatccgcag tgcggttaat tgaaatggaa aaagtatgaa
    28021 gaactggaaa acgctgcttc tcggtatcgc catgatcgcg aataccagtt tcgctgcccc
    28081 ccaggtagtc gataaagtcg cagccgtcgt caataacggc gtcgtgctgg aaagcgacgt
    28141 tgatggatta atgcagtcgg taaaactgaa cgctgcgcag gcaaggcagc agcttcctga
    28201 tgacgcgacg ctgcgccacc aaatcatgga acgtttgatc atggatcaaa tcatcctgca
    28261 gatggggcag aaaatgggag tgaaaatctc cgatgagcag ctggatcagg cgattgctaa
    28321 catcgcgaaa cagaacaaca tgacgctgga tcagatgcgc agccgtctgg cttacgatgg
    28381 tctgaactac aacacctatc gtaaccagat ccgcaaagag atgattatct ctgaagtgcg
    28441 taacaacgag gtgcgtcgtc gcatcaccat cctgccgcag gaagtcgaat ccctggcgca
    28501 gcaggtgggt aaccaaaacg acgccagcac tgagctgaac ctgagccaca tcctgatccc
    28561 gctgccggaa aacccgactt ctgatcaggt gaacgaagcg gaaagccagg cgcgcgccat
    28621 tgtcgatcag gcgcgtaacg gcgctgattt cggtaagctg gcgattgctc attctgccga
    28681 ccagcaggcg ctgaacggcg gccagatggg ctggggccgt attcaggagt tgccgggtat
    28741 cttcgcccag gcattaagca ccgcgaagaa aggcgacatt gttggcccga ttcgttccgg
    28801 cgttggcttc catattctga aagttaacga cctgcgcggc gaaagcaaaa atatctcggt
    28861 gaccgaagtt catgctcgcc atattctgct gaaaccgtcg ccgatcatga ctgacgaaca
    28921 ggcccgtgtg aaactggaac agattgctgc cgatatcaag agtggtaaaa cgacttttgc
    28981 tgccgcagcg aaagagttct ctcaggatcc aggctctgct aaccagggcg gtgatctcgg
    29041 ctgggctaca ccagatattt tcgatccggc cttccgtgac gccctgaccc gcctgaacaa
    29101 aggtcaaatg agtgcaccgg ttcactcttc attcggctgg catttaatcg aactgctgga
    29161 tacccgtaat gtcgataaaa ccgacgctgc gcagaaggat cgtgcatacc gcatgctgat
    29221 gaaccgtaag ttctcggaag aagcagcaag ctggatgcag gaacaacgcg ccagcgccta
    29281 cgttaaaatc ctgagcaact aatggttaaa acccaacgtg ttgtgatcac tcccggcgag
    29341 cccgccggga ttggcccgga cttagttgtc cagcttgcac agcgtgagtg gccggtcgaa
    29401 ctggttgttt gtgccgatgc cactctcctt accgaccggg cagcgatgct cggtttgccg
    29461 ctcaccctcc gcccttattc ccccaactcc cctgcacaac cgcaaactgc gggcacatta
    29521 acgctacttc ctgtcgcgct acgcgaatct gtcactgcgg ggcagttagc gattgaaaat
    29581 gggcattacg tggtggagac gctggcgcga gcgtgtgatg gctgtctgaa cggtgaattt
    29641 gctgcgctga tcacaggccc cgtgcataaa ggcgtcatta acgacgcagg tattccgttt
    29701 actggtcata ccgagttttt cgaagagcgc tcgcaggcga aaaaagtggt gatgatgctg
    29761 gcgaccgaag aacttcgcgt ggcgctggca acgacgcatt taccgctgcg cgatatcgca
    29821 gatgctatca cccctgcact tttgcacgaa gtgattgcta ttttgcatca cgatttgcgt
    29881 acaaaatttg gtattgccga accgcgcatt ctggtctgcg ggctgaatcc gcacgcgggc
    29941 gaaggcggtc atatgggtac ggaagagata gacaccatta ttccggtgct cgacgagctg
    30001 cgggcgcagg ggatgatact caacgggccg ctgcctgccg ataccctgtt tcagccgaaa
    30061 tatctcgata acgccgacgc cgtgctggcg atgtaccacg atcagggtct tcccgtgcta
    30121 aaataccagg gcttcgggcg cggtgtgaac attacgctgg gcctgccctt tattcgcaca
    30181 tcagtggacc acggcaccgc gcttgaactg gcgggacgtg gcgaagccga tgtcggcagt
    30241 tttattacgg cgcttaatct cgccatcaaa atgattgtta acacccaatg aataatcgag
    30301 tccaccaggg ccacttagcc cgtaaacgct tcgggcaaaa ctttctcaac gatcagttcg
    30361 tgatcgacag cattgtttct gccattaacc cgcaaaaggg ccaggcaatg gtcgaaatcg
    30421 gccccggtct ggcggcattg accgagccgg tcggcgaacg tctggaccag ctgacggtca
    30481 tcgaacttga ccgcgatctg gcggcacgtc tgcaaacgca tccattctta ggcccgaaac
    30541 tgacgattta tcagcaggat gcgatgacct ttaactttgg cgaactggcc gagaaaatgg
    30601 gtcagccgct gcgtgttttc ggcaacctgc cttataacat ctccacgccg ttgatgttcc
    30661 atctgtttag ctatactgat gccattgccg acatgcactt tatgttgcaa aaagaggtgg
    30721 tgaatcgtct ggttgcagga ccgaacagca aagcgtatgg tcgattaagc gtcatggcgc
    30781 aatactattg caacgtgatc ccggtactgg aagttccgcc gtcagccttt acaccaccac
    30841 ccaaagtgga ttccgccgtc gtgcgcctgg ttcctcatgc aacgatgcct cacccggtta
    30901 aagatgttcg tgtgttgagc cgcatcacca ccgaagcctt taaccagcgt cgtaaaacca
    30961 ttcgtaacag cctcggcaac ctgtttagcg tcgaggtgtt aacgggaatg gggatcgacc
    31021 cggcgatgcg agcggaaaat atctctgtcg cgcaatattg ccagatggcg aactatctgg
    31081 cggagaacgc gcctttgcag gagagttaac gatgatcaat tcgccccgag tgtgtattca
    31141 ggttcaaagc gtctacattg aggcacaatc ttcacctgat aatgaacgtt acgtttttgc
    31201 ttataccgta accatacgca atctggggcg agcgccagtg cagttgttgg ggcgttactg
    31261 gctgatcacc aatggcaatg gccgtgaaac cgaagtccag ggcgaaggag tggttggcgt
    31321 ccagccactt atcgcgcctg gcgaagagta ccagtacacc agcggtgcaa tcattgaaac
    31381 cccgctgggc accatgcagg gtcactacga aatgatcgat gaaaatggcg tccctttcag
    31441 catcgacatt cccgtattcc gactcgccgt tcccacactc attcattaaa agaatatggc
    31501 gacatacctt attggcgacg ttcatggttg ttacgatgaa ctgatcgcat tgctgcataa
    31561 agtagaattt acccctggga aagataccct ctggctgacg ggcgatctgg tcgcgcgcgg
    31621 cccgggttcg ctggatgttc tgcgctatgt gaaatcctta ggcgacagcg tacgtctggt
    31681 gctgggcaat cacgatctgc atctgctggc ggtatttgcc gggatcagcc gcaataaacc
    31741 gaaagatcgc ctgacaccgc tgctggaagc gccggatgcc gacgagctgc ttaactggct
    31801 gcgtcgccag cctttgctgc aaatcgacga agagaaaaag ttggtgatgg cccacgcagg
    31861 gatcacgccg cagtgggatc tacagaccgc caaagagtgc gcgcgcgatg tagaagcggt
    31921 gctatcgagt gactcctatc ccttctttct cgacgctatg tacggcgata tgccaaataa
    31981 ctggtcaccg gaattgcggg ggctgggaag actgcgtttt atcaccaacg cctttacccg
    32041 tatgcgtttt tgcttcccga acggtcaact ggatatgtac agcaaagaat cgccggaaga
    32101 ggcccctgcc ccactgaaac cgtggtttgc gattcctggc cctgtcgctg aagaatacag
    32161 catcgccttt ggtcactggg catcgctgga gggcaaaggt acgccggaag gtatatacgc
    32221 gctggatacc ggctgctgct ggggtggtac attaacctgc ctgcgctggg aagataaaca
    32281 gtattttgtc cagccgtcga accggcataa ggatttgggc gaagcggcgg cgtcttaaac
    32341 acagcctgat ataggaaggc cggataagac gcgaccggcg tcgcatccgg cactagccgt
    32401 aaattctata caaaattacc gccgctccag aatctcaaag caatagctgt gagagttctg
    32461 cgcatcagca tcgtggaatt cgctgaatac cgattcccag tcatccggct cgtaatccgg
    32521 gaaatgggtg tcgccttcca cttctgcgtc gatatgcgtc agatacagtt tctgcgcttt
    32581 tggcaggaac tgctcataaa cgcgaccgcc gccaatcacc atgatttctg gtacgtcacc
    32641 acacgccgcg atggcttcat ccaccgactt cacccacgtt acgcgatcgt ccgtacccgg
    32701 ttgactgctg aggataatat ttttgcgtcc tggcaacgga cgaccgattg attcccaggt
    32761 atggcggccc ataatcacgg gtttatttaa ggtgttgcgt ttaaaccagg cgagatcggc
    32821 aggcaggttc cacggcatgg cgttttccat gccgataacg cgatctaccg ctaacgccgc
    32881 aatcagactg atcattgaga tttcccgata aaaaaaattg tcgccactat acgtaaagcg
    32941 taaaccgtcg tcgactggtg cgaggatgat gttgaggaaa attttatatt ctgctggcga
    33001 gtccacgctc tctccctgga ctcgccgcat tacaatgaaa caaaaacaaa cagttagctg
    33061 taaagtgtga tttatgtcac tctttattag gatgagggtt tcgtttccgg ttcatccgcc
    33121 atgttgccgg tatgtttacc ttcttcggtt ccctgccagc catgtcgttg aattaatgac
    33181 agatgttcgc ggtcctcggt aatgatctca cttaacatcg cgctggtgcg tttatagacc
    33241 gccgcgcggg ctttggtgtc gttctcaacc attgccatct cttccaccat ctgaatatta
    33301 aagcggcgga acacatcggc acgttctcgc gcttcatacg gccccagacc taaactttcc
    33361 agtgccagac gcccggtttt cagcgcacct tcgaaggttt cacgctccgg cttttcaacg
    33421 cctgcctgac gcaaacgaat gtagtggtcg acatcgcggg cgcgggcaat aatctgcaaa
    33481 tgcgggaagt gttctttcac catctctgtc agttgcaggt tggtttgcgg atcgtcgatg
    33541 gcgttaatca gcacttccgc tttcgccgct cccgcagatt ccagtaaatc catccgcgtg
    33601 gcatcgccat aaaacacttt cataccaaat ttacgcaagg tttcgatatg gtccggatcg
    33661 tgatcgagca ccaccatttt caccccgctg gagagcagta aacgtccggt gatctgccca
    33721 aaacgaccga atccggcaat gattacccgc ggctgttctt cgtcgatctc atcggcttca
    33781 cgcgcctctt cagtagaaga ttgctcaagg cgattgagga tcaccagcag aatcggcgtt
    33841 gccgccatcg acagcgccac cgccagggtc agcgatttcg cccactccgg ctccagcaca
    33901 ttcgccatct gcgccgcgcc aaataccaca aaggcaaact cactgccctg ccctaacaac
    33961 accgcaaacc aacgacgctg tttatttggc acttgcaacg gtcgggcaat cagccacagc
    34021 atggcgattt tgatgatgag gaaaccgagc agcaaaatga caatgcgcaa tgggttttca
    34081 agcagcgtgc caaagtctat cgacatgcca acaccgatga aaaacagccc caacagcaaa
    34141 cctttaaagg gttcgatatc gctctccagc gcatgacggt attccgagct tgccagcagt
    34201 acgcccgcca gaaacgcgcc catcgccatc gacaagccga cctcttccag cagcaaacca
    34261 aagccaaaca cgaggaataa cgccacggca ctaaacactt cccgcaagcc agagcgggct
    34321 acaaaacgca gcgccggacg cgtgacatag cgccccagca ataccaccag caccagcgca
    34381 cccgccactt ttaacgccga gagagcaaat gcgcccatcg tcgtcgaggc actgctcgtt
    34441 gccagtagcg gaatcatcgc caccagcggg atcgccgcga tatcctggaa cagcagcacc
    34501 gcaaaggcac tgcgacccat ttgcgtcacc atcagattgc gttcattcat cgcctgcatg
    34561 gcaatcgccg tagaggagag cgccagcgtc atgccgatca attccgcgac ctgccagcgc
    34621 agcccaagta acatgcagaa cagccccagc aggccgccgc aaatcaccat ctgcaatgcg
    34681 ccaccgccga acactgccgc acgcagcttc cacagccttt gtggatcgag ttcgaggccg
    34741 ataataaaca gcatcagcac caccccaatc tcggcaaagt gcagaataga ttcggcatcg
    34801 gtcaccagtc gcagccccca cgggccaata atgcagccgg cgatcaggta gccaagtacc
    34861 gatcccagac caagacgtac cgcaatgggt acaatcagcg ctgccgaacc gagataaatc
    34921 agcgcctgaa tcagcgtatg gctatccatg atgggcctcc tgccattcca gcagacgttg
    34981 cttatagtga cgcgcctgcc cttcgagggt ttcgtcgtca caaataaagg tgcagtgcat
    35041 ggcaaacggt ggcagccagt tcagcccgca gtagattgcc gtcgcctgta gcggctgcga
    35101 cagcacatca aagcccggat gcgcaccaat ttcaaaatgg ctttccccgc cgccggtcgt
    35161 caccgcccac agcaaatgtt tgccatgcag cgccttgccg ccgtgaccgt aagcccagcc
    35221 gtgcgacaaa actttatcga tccaaagttt aagaagcgga ggaatgctgt accactgcat
    35281 cggatgctgc cagacgatca gatcggcgcg agacagcgcc tcctgctcgg cggcaatatc
    35341 gatattgaag tcaggataga gttgataaag agagcgaatt tcgacgcctt ccagcgtcct
    35401 tgcctgttca agcatccgtt tattcgcatg ggaatgatgc ggatacggat gcgcataaat
    35461 tataagaatc ataggtagcc cgcgtgatta cgcctttgtt ttcccacaaa gtgtagtcat
    35521 atattcacgc ggcttatagt caataatatt gattcagaaa agagagaaat ctgaacaatt
    35581 taccccattc tctgcccgcg gatcgtcata ttgctggttt gcgccaatga catccctcga
    35641 tgtctccggg gcaaacgcta cggaaatcaa caagccaaac agcgagatac ccgcccccat
    35701 tagcatcgtg ttactgatac cgtaattatt gataaagatc ggcagtgccc aggtcgaaac
    35761 aatggtgcca atacggctta aggacataat cacgcccacg gcagaggcgc ggatatctgt
    35821 cgggaagagt tcattaggat agagccactg caaattaccc ggcccgccag agaaaaaggc
    35881 atacaccgca aaggccatca ctaccagcca gatccccata tccgggatca gccccaaaac
    35941 cgccagcgcc agcgtcatca tggcaaagct gccaatcaac aatggacgcc gtccggcagt
    36001 gtttaaccac agcatcggcg gaatacagcc gagcataaag aacaggctaa tcaccacgtt
    36061 ccccagcgcc gcgtttttgc caacacccaa tcccaacaaa ccaacgattt gcgggccaaa
    36121 ggtgtaaatg gcgaacattg ggatcacctg gcaggtccag atggcggcaa caaacagaac
    36181 aaaaggaaaa tggcggcgat taaacagatc gcgaaaacgg gtttgctgcg gctgctcttc
    36241 atcgaaagcc accggttcgc caaacagttt tatcatcatc tcttcgcact cttttactcg
    36301 ccctttgcgt aataaccagc ggggagattc aggcagttcg aatcgaccaa tcaaaatcaa
    36361 caaacagggg atcgccgcgc tacccagcat ccagcgccag ccgccttcca catcataaag
    36421 ccagtagccg accagatcgg cgcaggtcgc gccgacatac cacatggcgg cgataaagct
    36481 gatggaaaat gcccgctgac gggtactgga gaactcggtg atcattgagg tggcgatggg
    36541 ataatcggca ccgatgacaa tgccgataag tacccgcatc accaacagtt cgacggggga
    36601 tgaaacaaac atcgtcgcca ccgatatcac gccgatggcg atgatatcaa tgaggaacat
    36661 tttgcgccgt ccgactttat cggaaatata gccaaacagc gatgtgccaa cgaacagccc
    36721 ggcgagcgtt cccgcgccca acaagccaat ccagtcagcg tccagtttta gcgccggagt
    36781 cagttgctcc agcgccacgc caatcattac cagtacataa ccatcca
//
LOCUS       DAJVUJ010000019        36494 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.019, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000019 DAJVUJ010000000
VERSION     DAJVUJ010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36494
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.019"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            92..988
                     /locus_tag="OQQ48_000909"
     CDS             92..988
                     /locus_tag="OQQ48_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1951762.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATMLQHIAEDRDGTNH"
     gene            complement(985..2463)
                     /gene="scpC"
                     /locus_tag="OQQ48_000910"
     CDS             complement(985..2463)
                     /gene="scpC"
                     /locus_tag="OQQ48_000910"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HCS1951763.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETDKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(2487..3272)
                     /gene="scpB"
                     /locus_tag="OQQ48_000911"
     CDS             complement(2487..3272)
                     /gene="scpB"
                     /locus_tag="OQQ48_000911"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HCS1951764.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(3283..4278)
                     /gene="meaB"
                     /locus_tag="OQQ48_000912"
     CDS             complement(3283..4278)
                     /gene="meaB"
                     /locus_tag="OQQ48_000912"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HCS1951765.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPYCGNTLRLGVTGTPSAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKIRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLIVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIEFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(4271..6415)
                     /gene="scpA"
                     /locus_tag="OQQ48_000913"
                     /pseudo
     CDS             complement(4271..6415)
                     /gene="scpA"
                     /locus_tag="OQQ48_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
     gene            complement(6619..7512)
                     /gene="argP"
                     /locus_tag="OQQ48_000914"
     CDS             complement(6619..7512)
                     /gene="argP"
                     /locus_tag="OQQ48_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HCS1951766.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            7895..8554
                     /gene="rpiA"
                     /locus_tag="OQQ48_000915"
     CDS             7895..8554
                     /gene="rpiA"
                     /locus_tag="OQQ48_000915"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HCS1951767.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            8809..10041
                     /gene="serA"
                     /locus_tag="OQQ48_000916"
     CDS             8809..10041
                     /gene="serA"
                     /locus_tag="OQQ48_000916"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCS1951768.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            10270..10329
                     /gene="ibsC"
                     /locus_tag="OQQ48_000917"
     CDS             10270..10329
                     /gene="ibsC"
                     /locus_tag="OQQ48_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HCS1951769.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            10662..10721
                     /locus_tag="OQQ48_000918"
     CDS             10662..10721
                     /locus_tag="OQQ48_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCS1951770.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(10822..11424)
                     /locus_tag="OQQ48_000919"
     CDS             complement(10822..11424)
                     /locus_tag="OQQ48_000919"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCS1951771.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(11445..11628)
                     /gene="ssrS"
                     /locus_tag="OQQ48_000920"
     ncRNA           complement(11445..11628)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="OQQ48_000920"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(11670..11999)
                     /gene="zapA"
                     /locus_tag="OQQ48_000921"
     CDS             complement(11670..11999)
                     /gene="zapA"
                     /locus_tag="OQQ48_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCS1951772.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            12167..12745
                     /gene="ygfB"
                     /locus_tag="OQQ48_000922"
     CDS             12167..12745
                     /gene="ygfB"
                     /locus_tag="OQQ48_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HCS1951773.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            12771..14096
                     /gene="pepP"
                     /locus_tag="OQQ48_000923"
     CDS             12771..14096
                     /gene="pepP"
                     /locus_tag="OQQ48_000923"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HCS1951774.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIMNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     AEVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            14093..15271
                     /gene="ubiH"
                     /locus_tag="OQQ48_000924"
                     /gene_synonym="visB"
     CDS             14093..15271
                     /gene="ubiH"
                     /locus_tag="OQQ48_000924"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HCS1951775.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            15294..16496
                     /gene="ubiI"
                     /locus_tag="OQQ48_000925"
                     /gene_synonym="visC"
     CDS             15294..16496
                     /gene="ubiI"
                     /locus_tag="OQQ48_000925"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HCS1951776.1"
                     /translation="MQCVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADISLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLVLVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(16570..16653)
                     /locus_tag="OQQ48_000926"
     CDS             complement(16570..16653)
                     /locus_tag="OQQ48_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1951777.1"
                     /translation="MSKNTKSKNNDIRKYNAKTEVKLVYFK"
     gene            16943..18037
                     /gene="gcvT"
                     /locus_tag="OQQ48_000927"
     CDS             16943..18037
                     /gene="gcvT"
                     /locus_tag="OQQ48_000927"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HCS1951778.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            18061..18450
                     /gene="gcvH"
                     /locus_tag="OQQ48_000928"
     CDS             18061..18450
                     /gene="gcvH"
                     /locus_tag="OQQ48_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HCS1951779.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            18569..21442
                     /gene="gcvP"
                     /locus_tag="OQQ48_000929"
     CDS             18569..21442
                     /gene="gcvP"
                     /locus_tag="OQQ48_000929"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HCS1951780.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLARAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETSISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSHEVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            21708..22451
                     /locus_tag="OQQ48_000930"
                     /pseudo
     CDS             21708..22451
                     /locus_tag="OQQ48_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(22508..23941)
                     /gene="bglA"
                     /locus_tag="OQQ48_000931"
     CDS             complement(22508..23941)
                     /gene="bglA"
                     /locus_tag="OQQ48_000931"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HCS1951781.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEIPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMINAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWCWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            23986..24297
                     /locus_tag="OQQ48_000932"
     CDS             23986..24297
                     /locus_tag="OQQ48_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HCS1951782.1"
                     /translation="MQPNDITFSQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELIKVIADIYPGQTQFYVIEFKC
                     L"
     gene            24461..25120
                     /locus_tag="OQQ48_000933"
     CDS             24461..25120
                     /locus_tag="OQQ48_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCS1951783.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSMCHFLAIYLYIGQ
                     A"
     gene            complement(25197..26177)
                     /gene="ygfZ"
                     /locus_tag="OQQ48_000934"
     CDS             complement(25197..26177)
                     /gene="ygfZ"
                     /locus_tag="OQQ48_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HCS1951784.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMTENQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAADDERVLLGVAGFQARAALANLFSELPSREKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            26420..26686
                     /gene="sdhE"
                     /locus_tag="OQQ48_000935"
     CDS             26420..26686
                     /gene="sdhE"
                     /locus_tag="OQQ48_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HCS1951785.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            26667..27074
                     /locus_tag="OQQ48_000936"
     CDS             26667..27074
                     /locus_tag="OQQ48_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin CptA"
                     /protein_id="HCS1951786.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(27114..27635)
                     /gene="fldB"
                     /locus_tag="OQQ48_000937"
     CDS             complement(27114..27635)
                     /gene="fldB"
                     /locus_tag="OQQ48_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HCS1951787.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            27747..28643
                     /gene="xerD"
                     /locus_tag="OQQ48_000938"
     CDS             27747..28643
                     /gene="xerD"
                     /locus_tag="OQQ48_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCS1951788.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            28668..29378
                     /gene="dsbC"
                     /locus_tag="OQQ48_000939"
     CDS             28668..29378
                     /gene="dsbC"
                     /locus_tag="OQQ48_000939"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000715205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HCS1951789.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGAAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLESQAEQQMKAIW
                     CAKDKNKAFDDVMAGKAATPASCDIDIADHYALGVQLGVSGTPAIVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGN"
     gene            29384..31117
                     /gene="recJ"
                     /locus_tag="OQQ48_000940"
     CDS             29384..31117
                     /gene="recJ"
                     /locus_tag="OQQ48_000940"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCS1951790.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQIEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            31208..32306
                     /gene="prfB"
                     /locus_tag="OQQ48_000941"
     CDS             join(31208..31282,31284..32306)
                     /gene="prfB"
                     /locus_tag="OQQ48_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCS1951791.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            32316..33833
                     /gene="lysS"
                     /locus_tag="OQQ48_000942"
     CDS             32316..33833
                     /gene="lysS"
                     /locus_tag="OQQ48_000942"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCS1951792.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITK
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(33876..34424)
                     /gene="idi"
                     /locus_tag="OQQ48_000943"
     CDS             complement(33876..34424)
                     /gene="idi"
                     /locus_tag="OQQ48_000943"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCS1951793.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTNSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            34479..34550
                     /locus_tag="OQQ48_000944"
     CDS             34479..34550
                     /locus_tag="OQQ48_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1951794.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(34547..34672)
                     /locus_tag="OQQ48_000945"
     CDS             complement(34547..34672)
                     /locus_tag="OQQ48_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1951795.1"
                     /translation="MNLLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(34674..36122)
                     /gene="uacT"
                     /locus_tag="OQQ48_000946"
     CDS             complement(34674..36122)
                     /gene="uacT"
                     /locus_tag="OQQ48_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HCS1951796.1"
                     /translation="MSAIDSQLLSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSVGL
                     SIIQVGIDWAAGGKGNPQYGNPVYSGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDALWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
BASE COUNT         8793 a         9184 c         9762 g         8755 t
ORIGIN      
        1 cccccggatc atggacgact gggggcagta atcatgccgg gtctggctgg ctaacacccc
       61 attataagga gtacgaataa agtaacgggg gatggacatt tttatttcga aaaagatgcg
      121 caatttcatt ttattagcgc aaaccaataa tattgcccgg gcggcagaaa aaatccatat
      181 gacggcttcg ccatttggta aaagcattgc cgcgctggaa gaacaaattg gctatacgct
      241 atttacccgc aaagataata atatcagcct caataaagcc ggtcaggaac tgtatcaaaa
      301 actgttcccg gtttatcaac gactttctgc gattgataat gaaatccata actcagggcg
      361 tcgttcacga gaaattgtga ttggcataga taatacttat ccgacaatta ttttcgatca
      421 gttgattagt ctcggagata agtatgaagg cgtgaccgcc cagccagttg agttcagcga
      481 aaacggcgtt atcgataatc tttttgaccg tcagttagat tttattatct ccccacagca
      541 tgtatctgca cgagttcagg aactggaaaa cctgactatc agtgaattac caccgctgcg
      601 attgggcttt ctcgtttctc gccgttatga agagcgtcaa gagcaagagc tgcttcagga
      661 gctgccctgg ctacaaatgc gctttcaaaa ccgggctaac ttcgaagcaa tgattgatgc
      721 gaatatgcga ccttgtggga tcaatcccac cattatttat cgtccctaca gttttatggc
      781 caaaatcagc gccgtggagc gcggacattt tctgacggtg atcccgcatt ttgcctggcg
      841 actggtgaat ccggcaacgc tgaaatattt tgatgcgccg cacaggccga tgtatatgca
      901 ggaatatctt tattcaatca gaaatcatcg ttataccgcc acgatgcttc agcatattgc
      961 tgaagatcgt gacgggacaa accattaacc cagcatcgag ccggttgcaa ttaaattacg
     1021 gtgtaagtcg aagacgtggc taagatcgtg gtgaatatgt ccgccgggcg cattttccag
     1081 atagcgatgc agataatccc gatacatagg atgtgcacaa ttatcaatga tagtgcgggc
     1141 gcgttgaagc ggggaaagac cgcgcagatc ggcgatccct tgttcggtga tgatcacttt
     1201 gacgctgtgt tcgctgtgat caacatggct gcacattggc acgacggttg agatcttgcc
     1261 ttctttagca atcgacggag ccataaagat cgacagatac gcgttgcgtt caaaatcacc
     1321 gctgccgccg atgccgttca tcagatcgac cccggctacg tgcgttgaat ttgcatgccc
     1381 gtaaatatca aactccaggc cgacgttcag agcgatgacg cccagacgac ggatgatttc
     1441 cgggttattg gaaatctcct gcggacgcaa cacaatgcgg ctggcaaagt aatccatatt
     1501 gtcgtaaatc ttgcgcaggg aatcggccga gattgtcagg ctggaggcgc tggccccgct
     1561 gattttgtcg gtttccagta aatgcaccac cgattcctgt agcacttccg aatacatcat
     1621 gaacggagga atttccgggt tttcccccag acgcgccatt accgcattat tgatattgcc
     1681 cacgccactt tgcagcggca gaaattccgg cggaatacgc ccatgcgcca tttcctgcaa
     1741 taagaacgtg accacgttat cggcaatctg ctggcacatg ggattttgct tatccagcat
     1801 attaccggcg tcgggcaagt tggtttccac gacggcgaca atctttttcg gatcgatttg
     1861 cacatagcgg gtaccgacgc gatccattgc atggaagatc gacacgctat tgcgccgcgg
     1921 tggcgcgcca ggaatcacaa tatctgccag ttctgcaacg cgcggatcgt gatagtgatt
     1981 aagttcaatg atcactttct tcgctcgcag cagccaggtc ggcgcattac cgatcccgct
     2041 ggttaaccag actcgaccat ccggtgccag tgccgatgct tcaatgacgg caacatcaat
     2101 gtcgccgaag aaaccgtaat tgaccatttg cgccacttcg ctcaaatgca ggtcaacgaa
     2161 actcaccgcg ccctgattga tctttttacg taaaccggac gatgtttgat atggcgcacg
     2221 ccaggaaaca gcatcggcgt cagaaagtac atcgtcagcg gcggcgctga ttgacgcccc
     2281 cgtcagcagg cgaatttgat acggcttttt ggcctcatgc tgttcgttag ctctgcgggc
     2341 aatcgcggtg ggtagggctt tcggcgaacc cgccggggta aagccgctaa atgccaccat
     2401 gtcgttatgc tggataattt ctgccgcttc atcggcggtc atccttgtcc actgagtttc
     2461 catttttact ccttcgttcg cagggattaa tgaccaacga aattaggttt acgtttttcg
     2521 aggaaagcgt tcattccttc ctggtaatct tcgctgtcat aaaccgcgcg tcgcatcccc
     2581 tgaatacgtt caaattcatc ggagttcatg gtgtgcgctt cgcccagtac acgcagctct
     2641 tctttgataa cagcaatggc taacggcgct ttctcagaga tgtggtgcgc catttgtaag
     2701 gtgaaatctt ccagttcttc cacttccaca acatggttga ggatgccgac agccagcgcg
     2761 cgctgggcgg tgattggcga agcggtaaaa atcagctctt tgacaatgtg gaagcccgcg
     2821 tcgcgggtca ggttgtgaat gccgaccagg ttatacggga cgccgaggtt tacaggcgtc
     2881 attgagaagg ttgaggtact ggcggcgatg atcagatcgg aactcatgat catttcaaat
     2941 gcgccacccc aaacactacc ttccaccatc gaaatgatcg gtttcgggaa tttttggatc
     3001 atgcgggtga tttgacgcaa tggatcatca taggagagcg gatcgcgacc gccagacggc
     3061 agttcgtgaa tatcgtgacc tgcggagaag actttggatc cactcggcgc gcgcaaaatg
     3121 atacagcgaa tttccggccg gttgagatcg cttaacgcct gcataagatc atcaataaag
     3181 actttactta aggcattaag ttttcggcca tagttaaact caatgaccgc cactttgttg
     3241 atagtggcaa cgttcacata ctgataagac ataaaaattc ctttaatcaa aatattgcgt
     3301 ctggataaat tcactgagct gccgcaggcc ggtgcgcggt gagagcgtat tgtttttgac
     3361 cgctaaaagc gtctggcggt aatagcgatc gaaatcttca ttcgcgaaca ggtgattcag
     3421 tacttcttct tcggtctgct tacgcagcca ttccaccgat tgttgttgcc gcacttgttg
     3481 taaacgacca ctggcagtta gcgcggtttt gaactcgatg atggcgtgcc agatctcatc
     3541 gattccacgt ttttccagtg cgctacaagt cagaacccgt ggctgccatt cgtcgtattt
     3601 acgtcgcaga atatgcaggg cactctcgta catatgccgg gcaatggcga cattggtatg
     3661 gttatcgcca tcgtctttgt tgataacgat cagatcagcc acttccatca gccctttttt
     3721 aatgccctgc agatcatcgc cgccaccggc aatttgcaac gagataaaac agtccaccat
     3781 gcgggcgact tctgtttccg actgcccgac gccaaccgtt tcgacaatca ctacgtcata
     3841 acccgccgct tcgcataaca gcattaattc ccgcgctcgc tgactggcac cgcccagatg
     3901 accggaggat ggtaccgggc gaataaacgc cgcttcggca cgcgccaggt cattcatgcg
     3961 gattttatcc ccgagaatgc taccgccagt gaccgggctg ctgggatcga ccgcaataac
     4021 cgcgaccttt aatccctctc gaatcaacaa catgccaaag gcctcaagaa aggtactttt
     4081 ccccgcgctg ggggtgccgg taacgcccag tcgcagggtg ttaccgcagt acggcataat
     4141 ggcatcaagc agctgcgtac ttagtgcctg atgacgcggg tgacggcttt ccaccagcgt
     4201 catggcctgg gcgagtgtgg cacgctcacc ctgacgtaag cggcgaatac tttctgccag
     4261 cgtggcttca ttaatcatga tgctggctta tcagattcag tacgtcgcgc acactgtcga
     4321 gcataggtgt acctggacca taaatcgccg ccacgccgcg ctcttgcagg aaggcgtaat
     4381 cctgcggtgg aatgacgcca ccggcgacta cgcagatatc ttcgcgtccc cattttttca
     4441 gcgcctcgac cagttccggg atcagcgttt tatgaccggc agccagtgag gatgcgccca
     4501 ctacgtgaac gtcattttct actgccaggc gggcgatctc ttcaggtgta gagaacatcg
     4561 ggcttaaatc tacgtcgaaa ccgagatcgg aataggcgct ggcgatcact ttcgagccgc
     4621 gatcgtgccc atcctggccc attttggcga tcagaatgcg caggcgacga ccattgtcgg
     4681 caaggaactg ctccgtttgc gcaacaatgg catcgaactc ggaggccgat ttctcagatt
     4741 gatgatagct ttgcgcaatc acgccggtaa cacactggct tggcaccaga taacggtcga
     4801 aagcggcttc cagcgcatcg gaaatttcac ccagggtggc gcgaacgcga gcggcattaa
     4861 cagcggcagc cagcaggttt tcgttatgct gtgcggcgtg agtcagggcg ttcaacgcgg
     4921 cggttacggc ggcatcatca cgggtggcgc gaatgcgttc cagcgaagca atttgctcgt
     4981 tacgcaccat cacgttgtcg atctcaagta catcggtttc gtcttcgtga tccagtttgt
     5041 acttgttgac accaacgatg acacgtttgc cctggtcgat aagcgactgt tcgcgcgctg
     5101 aggcctattc gatcattcgt tttggcagac ctgcttcgat cgctttcgcc ataccaccgg
     5161 cttcgtcgat ctgttggata atagctctgg cttgtttgac gatttgatcg gtcagcgact
     5221 caatgtaata ggatccggcc agtggatcga cggtgcggca gagttctgat tcttcctgga
     5281 tgatgatctg ggtgttgcgg gcaatgcgtg ctgagaaatc ggtaggcaaa ccaagcgctt
     5341 cgtcaaaggc gttggtatgc agtgactgag taccgcccag cgtcgcagcc agtgcttcaa
     5401 tggtggtgcg gataacgttg ttatacggat cctgttcagt caggctccag cctgaggtct
     5461 ggcagtgggt acgcagcgcc agtgatttcg ggtcctgtgc gccaaatcca ctgaccgctt
     5521 cgctccatca ataacgtgcc gcacgcaaca tggcgacgtt cataaacaga tccatgccga
     5581 tgccgaagaa gaacgacagg cgaggagcga agtcatcaat tttcagtccg gcagagattg
     5641 ctgctttgat gtactcaatc ccatcagcga gcgtaaatgc tacctgctgc acgcagttgg
     5701 cacccgcttc acccatgtgg taaccgctga tactgatggt attaaatcgc ggcatgttgc
     5761 cggaacacca ggcgatgatg tcggcgataa tgcgcattga cggttttggc gggtaaatat
     5821 aggtgttgcg gcagaggtac tctttgagga tatcgttctg aatggtgccg gtcagtttat
     5881 caggtgtaac accttgctct tccgcggcga cgatataaaa cgccagtact ggtagcactg
     5941 cgccattcat ggtcatcgaa accgacattt tatccagcgg gatctggtcg aacaggactt
     6001 tcatatcttc cacggtgtcg atagcgacgc ccgctttgcc gacgtcgccc gccacgcgcg
     6061 ggttatcgga gtcgtagcct cggtgggtgg caaggtcaaa cgcaacggaa agaccttttt
     6121 gcccggcggc caggttacgg cgataaaaag cgttggactc ttttgctgtt gaaaaaccag
     6181 catactgacg gatggtccac ggttgggcgg tatacatagt ggcacgcggg ccacgaacgt
     6241 agggcggcaa accaggaagg gtacctgtca cctccagatt atcgagatcg gcttcggtat
     6301 acagcggctt gatggtgatc ccttccgcgg tttgctgaac cagcgagtcg acagttttct
     6361 cccgacggct caattccttg ttggcaagct gttgccactc ctgcacgtta gacattattt
     6421 gctccgtaaa acgcaatcaa aatgagggta tttgcgccta cggtgagctt tttacgtggg
     6481 tttgtcgcca gcaaaaaaaa tgcattcaga cgtcgttttt tgccgcctga gatttgtcta
     6541 ttttgtgatg tgattcaagg aatacaaaaa gaagtgagga caacgcctga tatgtgctat
     6601 caggcattat ttgatggatt aatcctgacg aaggactttg tgaccataat cgagcaacgc
     6661 atcagtgact ttacgcatca tgcggctttc aggagcaaag cggtgccagt agagcatccg
     6721 gcgttgaaat agccctggcg ttaagtcaat cagttcaccg ctggctagtt ctttctcgat
     6781 ttgcaggtgc gggatcatac agcaggtggt gccctggcga gcaagttgca cgaacgcttc
     6841 tgaagaatta acgatatggc agggcacgct gcctggaggc agatcgaagt tttgctgcaa
     6901 aaaggcctgg tgcatatcgt caagatggtc aaaggcgacc acaggtgctt tcaataatgc
     6961 cgaacgcgtt acgccgttag ggaaatattt ttcggcaaac ggttttgagc tgacgaacag
     7021 atagtcaagc gcaccgagct tatcgacaag gcaactcggc agcgcctgat gttgaatact
     7081 caccgcgccg accacttcgc cgcggcgcag acgttcctga gtacgggttt catcttctac
     7141 ctgcaagttg aggcggatag gcgaatcagc caatacagga gccagtgccg gaagcaacca
     7201 cgtcgccaga ctgtcggcgt tgaccgccag tgaaagcagc agcggagtcg aaccggtttg
     7261 ttcatcgccc agccactctt cttccagcaa ctccacctgg cgcagcagtg ccagcagttt
     7321 ttgcccttgt tccgtcgggc gcggcggtac ggtacgcacc aacagcggct gcccgaacat
     7381 attttccagt tgcttaatgc gctgtgagac ggctgattgt gtaatgcaca gcttttgtgc
     7441 cgcgcgctca aatcctcgtt cacgtatcac cgcatccagt gcctgtaatg ttctgtagtc
     7501 cgggcgtttc attgctgttg tctgctcctg gtaaatgctt tttcctgcac tatgacacaa
     7561 ttttgtgtca ggttgcatat accgcagccg tcggcgggta acatttgtat caatggttaa
     7621 acgcgcagct taaaagatca gggatttgcg ctattgcatt tcgcttagcg acttcgcgcc
     7681 ctggttatgt tgaatggcgt ggcgttattg cctctgttcg caccgtggag cggcataccc
     7741 agaccgttgt atgctttatt tgcggggcga aaggggatgc ccgccattgc gcgatatttt
     7801 atggatgagt taaccacccg gcttgccaac ggggtctgaa tcgctttttt tgtatataat
     7861 gcgtgtgaaa tttcatacca caggcgaaac gatcatgacg caggatgaat tgaaaaaagc
     7921 agtaggatgg gcggcacttc agtatgttca gcccggcacc attgttggtg taggtacagg
     7981 ttccaccgcc gcacacttta ttgacgcgct cggcacgatg aaaggccaga ttgaaggggc
     8041 cgtttccagt tcagatgctt ccactgaaaa actgaaaagc ctcggcattc acgtttttga
     8101 tctcaacgaa gtcgacagcc ttggcatcta cgttgatggc gcagatgaaa tcaacggcca
     8161 catgcaaatg atcaaaggcg gcggcgcggc gctgacccgt gaaaaaatca ttgcttcggt
     8221 tgcggaaaaa tttatctgta ttgccgacgc ctccaagcag gttgatattc tgggtaaatt
     8281 cccgctgcca gtagaagtta tcccgatggc acgtagtgca gtggcgcgcc agctggtgaa
     8341 actgggcggt cgtccggaat accgtcaggg cgtggtgacc gataatggca acgtgatcct
     8401 cgacgtccac ggcatggaaa tccttgaccc gatagcgatg gaaaacgcca taaatgcgat
     8461 tcctggcgtg gtgactgttg gcttgtttgc taaccgtggc gcggacgttg cgctgattgg
     8521 cacacctgac ggtgtcaaaa ccattgtgaa atgatctgac gggggaacct cccccgttaa
     8581 aaaaattctc ttcattaaat ttggtgacat gtgtcacgtt tttacctggc tattgtcgat
     8641 tgctctaaat aaatcctcta aaccagcata ttcaaccaag aaataccttt gcgtgatatt
     8701 tcctcaacat cgcgacgcaa acgttcatat tgccgcaata ttattttttg atatgttgaa
     8761 aggcggatgc aaatccgcac acaacatttc aaagacagga ttgggtaaat ggcaaaggta
     8821 tcgctggaga aagacaagat taagtttctg ctggtagaag gcgtgcacca aaaggcgctg
     8881 gaaagccttc gtgcagctgg ttacaccaac atcgaatttc acaaaggcgc gctggatgat
     8941 gaacaattaa aagaatccat ccgcgatgcc cacttcatcg gcctgcgatc ccgtacccat
     9001 ctgactgaag acgtgatcaa cgccgcagaa aagctggtcg ctattggctg tttctgtata
     9061 ggaacaaacc aggttgatct ggatgcggcg gcaaagcgcg ggatcccggt atttaacgca
     9121 ccgttctcaa atacgcgctc tgttgcggag ctggtgattg gcgaactgct gctgctattg
     9181 cgcggcgtgc cggaagccaa tgctaaagcg caccgtggcg tgtggaacaa actggcggcg
     9241 ggttcttttg aagcgcgcgg caaaaagctg ggtatcatcg gctacggtca tattggtacg
     9301 caattgggca ttctggctga atcgctggga atgtatgttt acttttatga tattgaaaat
     9361 aaactgccgc tgggcaacgc cactcaggta cagcatcttt ctgacctgct gaatatgagc
     9421 gatgtggtga gtctgcatgt accagagaat ccgtccacca aaaatatgat gggcgcgaaa
     9481 gaaatttcac taatgaagcc cggctcgctg ctgattaatg cttcgcgcgg tactgtggtg
     9541 gatattccgg cgctgtgtga tgcgctggcg agcaaacatc tggcgggggc ggcaatcgac
     9601 gtattcccga cggaaccggc gaccaatagc gatccattta cctctccact gtgtgaattc
     9661 gacaacgtac ttctgacgcc acacattggc ggttcgactc aggaagcgca ggagaatatc
     9721 ggcctggaag ttgcgggtaa attgatcaag tattctgaca atggctcaac gctctctgcg
     9781 gtgaacttcc cggaagtctc gctgccactg catggtggtc gtcgtctgat gcacatccac
     9841 gaaaaccgtc cgggcgtgct aactgcgctg aacaaaatct tcgccgagca gggcgtcaac
     9901 atcgccgcgc aatatctaca aacttccgcc cagatgggtt atgtagttat tgatattgaa
     9961 gctgacgaag acgttgccga aaaagcgctg caggcaatga aagctattcc gggtaccatt
    10021 cgcgcccgtc tgctgtacta attccccttc tctgaaaatt aacgggcagg tcactgactt
    10081 gcccgttttt ttatcccttc tccacaccgt tgccgcgttg cgcattctac tttgcgagtc
    10141 tgctccagaa aataaatcaa agtcagatca atgcgttgtg tttatttaat ggggatttac
    10201 gatggcggga cagtatgggg ctgtaacggt aaagccctca ccgaagcgag ggcttaaagg
    10261 agaagggtta tgatgcgact tgtcatcata ctgattgtac tgttactgat aagtttcgga
    10321 gcttattaac agtcagtctc tggggaggag tcatcctccc ttacccttac tcactaaatt
    10381 aggtcaaaga atcaacgatg tcaatcaggg cgatgcgggg tgcatcgccc tgaaccgaac
    10441 tttttgatag ccacttcatc gcccacgttt actgccgtaa tagatccttt ttcccaaaaa
    10501 ttatctctgt tttgcatggg gtttttcgaa cacgctcgaa gaaaagattt aaaggtagat
    10561 caatgcgttg tggttattta aagagagctt acgatagctg ggcagcatgg ggctgtaacg
    10621 gtaaagccct caccgaagcg agggcttgaa ggagaagggt tatgatgcga cttgccatca
    10681 tactgattgt actgttactc ataagtttcc cagcttatta acagtcagtc acaggggagg
    10741 agcaatcctc ccttaccctt actcactaaa ttaggttaaa gaatcaacga tgtcaatcag
    10801 ggcgatgcag gtgtatcgcc cttaccactc ccagactttc gacggtgtaa ccaccgcagg
    10861 aagagggata tcccactctt caacggggag tttttccacc aactgacaat catgcgcata
    10921 acccaccggt tgcgttttat agtgctgcca gttttgtaag gtccgatcat aaaaaccacc
    10981 gcccattccc aggcgctgac cgtactcatc aaaggcgacc agcggtgtga tcagcacgtc
    11041 taatcgggaa aggggtagca cgtcacgcac atccagtttt ggctcatgga ttttcaacct
    11101 gttcatcacc agttcgcttt gcggatggta attcaggaac agcaaattac cagcactaaa
    11161 gggatgcaaa actggaagat atacgcgctt accggcgcgc cagagttgtt ctattagcgg
    11221 ctgggtgtcg agttcaccat caaaagagag gaatacagcg accgtatgcg ccatcaccac
    11281 cgggggataa gtcatcatcc gggtagcggc ttgttgaccc atttcctgct gttgttccgg
    11341 cgttaacgca cgacgacgtt gccgaatcat tttgcggatt tcttgtcggg ataatgtcag
    11401 tgggagttct ggtagttgcg tcatggctgg tgccagataa gaagggaatc tccgagatgc
    11461 cgccgcaggc tgtaaccctt gaacccttgg ttcaaggtga atgtgtcgtc gcagttttaa
    11521 ggcttctcgg acggaccgag catgctcacc aaccgcggag cgccacattc ttgtggtatg
    11581 aaatatcggc tcaggggact ggcccgcttg cgaacatctc agagaaattt tgtcttcacg
    11641 gttactctac cacagtaaac cgaaaagtgt cattcaaagt tttggttagt tttttcggtg
    11701 atgcgacctt gttcaagtaa cgcttgttct atggtctgct gcagcatccg aatacgctgt
    11761 tccatacttg ccgcgtagtc acgagtcttc gctttttctt gcgctaactc atagctgata
    11821 ttcaatgcgg caatgaagac caactgttca gtatttgtga ctctagtgcg ttctttcaga
    11881 tcttgcaacc gttggttcag atcgtccgct gcctgattca acgcatccct ttggtcaggc
    11941 gggcagttca cacgcagtga acggccaaaa atttggatat cgacgggttg tgcagacatg
    12001 ccaccttcct gctgattgac tgcgctgcct tcgtctttag acccatggtc tgcgaagggg
    12061 cgacactata gctaccctga tgagaagaga caagcccttt tctggtccac cagggcccaa
    12121 agtggtagca tatcatgaat attcctccct ttgacgacga atgcttatgt ctatacagaa
    12181 cgaaatgcct ggttacaacg aaatgaacca gtatctgaac caacaaggga cgggtctgac
    12241 ccctgccgag atgcatggtt taatcagtgg gatgatatgt ggcggtaacg atgacagctc
    12301 atggctgccg ctacttcacg acctgacgaa cgaaggcatg gctttcggtc atgagctggc
    12361 acaggcactg cgtaaaatgc actctgccac cagcgatgcc ctgcaggatg acggcttcct
    12421 ttttcagctt tatctgcctg atggcgatga tgtcagcgtt ttcgatcggg ctgatgcatt
    12481 ggcaggttgg gtcaatcact tcctgcttgg tcttggcgtt acgcaaccga agctggataa
    12541 agtgaccggc gaaaccggtg aagctatcga cgatctgcgt aacattgcgc aactgggtta
    12601 cgacgaagac gaagatcagg aagagcttga aatgtcgctt gaagagatca tcgaatacgt
    12661 tcgtgttgcc gcgctgttat gccacgacac ctttactcat ccgcaaccga ccgcgccaga
    12721 agtacaaaaa ccgactctac actaaaaaca aaaaacgtaa ggagagtgtt atgagtgaga
    12781 tatcccggca agagtttcag cgtcgccgtc aggccctggt ggagcaaatg caacccggca
    12841 gcgccgcgct gatttttgct gcaccagaag taacacgtag cgccgacagc gaatacccct
    12901 atcgtcagaa cagtgacttc tggtacttca ccggctttaa cgaaccggaa gcggtgctgg
    12961 tgctgattaa aagcgatgac actcataacc acagcgttct gtttaaccgc gttcgcgacc
    13021 tgacggcgga gatctggttt ggccgtcgct taggccagga tgccgcgcca gagaaactgg
    13081 gcgttgaccg cgcactggca ttcagcgaaa tcaatcagca actttatcaa ctacttaacg
    13141 gcctggatgt ggtttaccat gcccagggcg aatatgcata tgctgatgaa atcatgaaca
    13201 gtgcgctgga aaaactgcgt aaaggttcgc ggcaaaatct caccgcaccg gcaacgatga
    13261 tcgactggcg tcctgttgtt catgaaatgc gcctgttcaa atcgccagaa gagattgccg
    13321 tactccgccg cgcgggagaa atcaccgcca tggcacatac acgggcgatg gaaaaatgcc
    13381 gtccgggaat gttcgagtac catctggaag gcgaaattca ccacgaattt aaccgccacg
    13441 gtgcgcgcta tccgtcctat aacaccattg tcggcagcgg tgaaaacggc tgcattctgc
    13501 actacaccga aaacgagagt gaactgcgcg acggtgacct ggtgttgatt gacgcgggtt
    13561 gtgaatacaa aggttacgcg ggcgatatta cccgcacctt cccggtcaac ggcaaattca
    13621 cccaggccca gcgtgaaatc tacgacattg tgctggagtc tctcgaaacc agcctgcgcc
    13681 tgtatcgtcc gggaacttcc attctggaag tcactggtga agtggtgcgc atcatggtta
    13741 gcggcctggt aaaactcggc attctgaaag gtgatgttga tgaactgatc gctcagaatg
    13801 cccatcgtcc tttctttatg catggcctta gccactggtt agggctagat gtccatgacg
    13861 ttggcgttta tggtcaggat cgctcgcgca ttctggaacc gggcatggta ctgaccgtag
    13921 agccagggct gtatattgcg ccggatgcag aagtgccaga acaatatcgc ggtatcggca
    13981 ttcgtattga agacgacatt gtgattaccg aaaccggtaa cgaaaacctc accgccagcg
    14041 tggtgaaaaa gccggaagaa atcgaagcgt tgatggctgc tgcgagaaag caatgagcgt
    14101 aatcatcgtc ggtggcggca tggcgggcgc gacgctggcg ctggctattt cccggttaag
    14161 tcacggggcg ctgccggtac atttgattga agcgactgcg ccagagtcac atgctcatcc
    14221 gggctttgat ggacgagcga tagcgctggc ggcgggtacc tgtcagcaac tggcgcgcat
    14281 cggcgtctgg caatctctgg cggattgcgc aactgccatc accaccgtgc atgtcagcga
    14341 tcgtggtcac gctggatttg tcaccctcgc cgcagaagat taccaactgg cggcgctggg
    14401 acaggttgtc gaattgcaca atgtcgggca acggctgttt gcattgctgc gtaaagcacc
    14461 tggcgtaacg ctgcattgcc ctgatcgcgt ggctaacgtt gcccgtactc agagtcacgt
    14521 tgaagtgacg ctggaaagtg gcgagacgct gacgggccgc gtgctggtag cagctgatgg
    14581 cacccattca gcgttagcca ccgcgtgcgg cgttgactgg cagcaggagc cttacgaaca
    14641 actggccgtg attgccaacg ttgcgacttc cgttgcgcac gaaggccgcg cttttgaacg
    14701 cttcacgcaa catggcccgc tggcgatgtt gccgatgtct gacggacgct gttcgctggt
    14761 ctggtgtcat ccactggaac ggcgcgaaga ggtgctgtcg tggagcgacg agaagttttg
    14821 ccgtgaactc cagtcggcct ttggctggcg acttggaaaa attacccacg ccggtaaacg
    14881 cagtgcttat ccgctggcgt taacccgcgc cgccagagct attacccatc gtaccgtgct
    14941 ggtgggcaat gcggcgcaaa ctctgcaccc gattgccggg caagggttta acctcggtat
    15001 gcgagatgtc atgagccttg cggaaaccct gactcaggcg catgagcgcg gagaagacat
    15061 cggtgattac ggcatattgt gccgctatca gcagcgtcga cagagcgatc gcgaagcaac
    15121 catcggcgtc acggacagcc tggtacatct ttttgccaac cgttgggcac cgctggttgt
    15181 cgggcgcaac atcggactga tgacgatgga attattcacc ccggcacgcg atgtgctggc
    15241 gcagcgcacc ctcggttggg tggcgcgttg aaggcggttt taaggaataa acaatgcaat
    15301 gtgttgatgt agccattgtt ggtggcggca tggtggggct ggcggttgcc tgcggcttac
    15361 aggggagcgg cttacgcgtt gccgtactgg agcagcgcgt accggaacct ctggcggcgg
    15421 atgcaccacc acaactgcgc gtttcggcta tcaatgccgc cagcgaaaaa ttactcaccc
    15481 gtcttggcgt ctggcaggac attctctctc gtagggccag ttgttatcac ggtatggaag
    15541 tgtgggacaa agacagtttt ggtcacattt cgtttgacga tcaaagcatg ggctatagcc
    15601 atcttgggca tatcgttgaa aactcagtga ttcactacgc gctgtggaac aaagcgcagc
    15661 agtcgtcaga tatcactctg ttggcccccg cagaattaca gcaggtcgcc tggggagaaa
    15721 atgaaacttt cctgacgctg aaagatggca gcatgttaac ggcgcgtctg gtgattggcg
    15781 cggacggtgc taattcctgg ttgcgcaaca aagcagatat ttcgctgact ttctgggatt
    15841 atcagcatca cgcgctggta gcgaccattc gcacggaaga accgcatgat gcggtggcgc
    15901 ggcaggtttt ccatggcgaa ggcattctgg cctttttacc gcttagcgat ccgcatcttt
    15961 gctcaattgt ctggtcactg tcgccagagg aagcgcagcg gatgcagcag gcaagtgaag
    16021 atgaatttaa tcgcgcgtta aatatcgctt ttgataatcg cctgggctta tgcaaggttg
    16081 agagcgcgcg tcaggtgttc ccactgacgg ggcgttatgc gcgccagttt gccgcgcacc
    16141 gtctggtgct agtgggcgac gccgcgcata ccattcaccc gctggcgggg cagggagtaa
    16201 atctcggctt tatggatgct gcagagctga ttgctgaact gaaacggttg catcgtcagg
    16261 ggaaagacat cgggcagtac atttacctgc gccggtatga gcgtagccgc aagcacagtg
    16321 cggcgctgat gctggctggt atgcagggat tccgcgatct gttttccggt accaatccgg
    16381 cgaaaaaact gctgcgtgat attggtttga aactggccga cacgcttcct ggcgttaaac
    16441 cacaacttat ccgccaggca atgggattaa acgatttgcc tgaatggctg cgttaaaaat
    16501 ttctcttctg ctgtttattt gatacccatc acactttcat ctcccggttt tttcgccggg
    16561 agattttcct catttgaaat aaactaattt cacctccgtt ttcgcattat attttctaat
    16621 gtcattattt tttgatttag tgttttttga cattttttta gctcttaata ttgccttatt
    16681 caaattagct ttctaatggc atcatcttta tatagaaact ggtgtgtttt ttggtttttt
    16741 attctgtcgc gattttggcg ttttttaacc ataagctaat gtgatgatca attttacctt
    16801 atggttaaca gtctgtttcg gtggtaagtt caggcaaaag agaacgattg cgttggggac
    16861 cgggagtggc tccgatgctg ggtttcgtgg tgataatttc accatgaaaa gttgtcagcc
    16921 ccgcttattc aatgaggaca agatggcaca acagactcct ttgtacgaac aacacacgct
    16981 ttgcggcgct cgcatggtgg atttccacgg ctggatgatg ccgctgcatt acggttcgca
    17041 aatcgacgaa catcatgcgg tacgtaccga tgccggaatg tttgatgtgt cacatatgac
    17101 catcgtcgat ctccgcggca gccgcacccg ggagtttctg cgttatctgc tggcgaacga
    17161 tgtggcgaag ctcaccaaaa gcggcaaagc cctttactcg gggatgttga atgcctctgg
    17221 cggtgtgata gatgacctca tcgtctacta ctttactgaa gatttcttcc gcctcgttgt
    17281 taactccgcc acccgcgaaa aagacctctc ctggattacc caacacgctg aacctttcgg
    17341 catcgaaatt accgttcgtg atgacctttc catgattgcc gtgcaagggc cgaatgcgca
    17401 ggcaaaagct gccacactgt ttaatgacgc ccagcgtcag gcggtggaag ggatgaaacc
    17461 gttctttggc gtgcaggcgg gcgatctgtt tattgccacc actggttata ccggtgaagc
    17521 gggctatgaa attgcgctgc ccaatgaaaa agcggccgat ttctggcgtg cgctggtgga
    17581 agcgggtgtt aagccatgtg gcttgggcgc gcgtgacacg ctgcgtctgg aagcgggcat
    17641 gaatctttat ggtcaggaga tggacgaaac tatttctcct ttagccgcca acatgggctg
    17701 gactatcgcc tgggaaccgg cagatcgtga ctttatcggt cgtgaagccc tggaagtgca
    17761 gcgtgagcat ggtacagaaa aactggttgg tctggtgatg accgaaaaag gcgtgctgcg
    17821 taatgaactg ccggtacgct ttaccgatgc gcagggcaac cagcatgaag gcattatcac
    17881 cagcggtact ttctccccga cgctgggtta cagcattgcg ctggcgcgcg tgccggaagg
    17941 tattggcgaa acggcgattg tgcaaattcg caaccgtgaa atgccggtta aagtgacaaa
    18001 acctgttttt gtgcgtaacg gcaaagccgt cgcgtgattt acttttttgg agattgattg
    18061 atgagcaacg taccagcaga actgaaatac agcaaagaac acgaatggct gcgtaaagaa
    18121 gccgacggca cttacaccgt tggtattacc gaacatgctc aggagctgtt aggcgatatg
    18181 gtgtttgttg acctgccgga agtgggcgca acggttagcg cgggcgatga ctgcgcggtt
    18241 gccgaatcag taaaagcggc gtcagacatt tatgcgccag taagcggtga aatcgtggcg
    18301 gtaaacgacg cactgagcga ttccccggaa ctggtgaaca gcgaaccgta tgcaggtggc
    18361 tggatcttta aaatcaaagc cagcgatgaa agcgaactgg aatcactgct ggatgcgacc
    18421 gcatacgaag cattgttaga agacgagtaa cggctttatt cctcttctgc gggagaggat
    18481 cagggtgagg aaaatttatg cctcaccctc actctcttcg taaggagaga ggttcacaat
    18541 tcactgcacg tttcaggaac catcgctcat gacacagacg ttaagccagc ttgaaaacag
    18601 cggcgctttt attgaacgcc atatcggacc ggacgccgcg caacagcaag aaatgctgaa
    18661 tgccgttggc gcacaatcgt taaacgcgct gaccggccag attgtaccga aagatattca
    18721 gcttgcgacc ccgccgcagg ttggcgcacc ggcgaccgaa tacgccgcac tggcagaact
    18781 caaggctatt gccagtcgca ataaacgctt cacgtcttac atcggcatgg gttacaccgc
    18841 cgtgcagcta ccgccggtta tcctgcgtaa catgctggaa aatccgggct ggtataccgc
    18901 gtacactccg tatcaacctg aagtctccca gggccgcctt gaagcactgc tcaacttcca
    18961 gcaggtaacg ctggatttga ctggactgga tatggcctct gcttctcttc tggacgaggc
    19021 caccgctgcc gccgaagcaa tggcgatggc gaaacgcgtc agcaaactga aaaatgccaa
    19081 ccgcttcttc gtggcttccg atgtgcatcc gcaaacgctg gatgtggtcc gtactcgtgc
    19141 cgaaaccttt ggttttgaag tgattgtcga tgacgcgcaa aaagtgctcg accatcagga
    19201 cgtcttcggc gtgctgttac agcaggtagg cactaccggt gaaattcacg actacactgc
    19261 gcttattagc gaactgaaat cacgcaaaat tgtggtcagc gttgccgccg atattatggc
    19321 gctggtgctg ttaactgcgc cgggtaaaca gggcgcggat attgtttttg gttcggcgca
    19381 acgcttcggc gtgccgatgg gctacggtgg cccacacgcg gcattctttg cggcgaaaga
    19441 tgaatacaaa cgctcaatgc cgggccgtat tatcggtgta tcgaaagatg cagctggcaa
    19501 taccgcgctg cgcatggcga tgcagactcg cgagcaacat atccgccgtg agaaagcgaa
    19561 ctccaacatt tgtacttccc aggtactgct ggcaaacatc gccagcctgt atgccgttta
    19621 tcacggcccg gttggcctga aacgtatcgc taaccgcatt caccgtttga ccgatatcct
    19681 ggcggcaggc ctgcaacaaa aaggtctgaa gctgcgccat gcgcactatt tcgacactct
    19741 gtgtgtggaa gtggccgaca aggcgggcgt actggcgcgt gccgaagcgg ctgaaatcaa
    19801 cctgcgtagc gatatcctga acgcggttgg gatcaccctt gatgaaacca ccacgcgtga
    19861 aaacgtaatg cagcttttca gcgtgctgct gggcgataac cacgggctgg agatcgacac
    19921 gctggacaaa gacgtggctc acgacagccg ctctattcag cctgcgatgc tgcgcgacga
    19981 cgaaatcctc actcatccgg tgtttaatcg ctaccacagc gaaaccgaaa tgatgcgcta
    20041 tatgcactcg ctggagcgta aagatctggc gctgaatcag gcgatgatcc cgctgggttc
    20101 ctgcaccatg aaactgaacg ccgccgccga gatgatccca atcacctggc cggaatttgc
    20161 cgaactgcac ccgttttgcc cgccggaaca ggctgaaggt tatcagcaga tgattgcgca
    20221 gctggctgac tggctggtga aactgaccgg ttacgacgcc gtttgtatgc agccgaactc
    20281 cggcgcacag ggcgaatacg cgggcctgct ggcgattcgt cattatcatg aaagccgcaa
    20341 cgaagggcat cgcgatatct gcctgatccc ggcttctgcg cacggaacta accccgcttc
    20401 tgcacatatg gcaggaatgc aggtggtggt tgtggcgtgt gataaaaacg gcaacatcga
    20461 tctgactgat ctgcgcgcga aagcggaaca ggcgggcgat aacctctcct gtatcatggt
    20521 gacttatccc tctacccacg gcgtgtatga agaaacgatc cgtgaagtgt gtgaagtcgt
    20581 gcatcagttc ggcggtcagg tttaccttga tggcgcgaac atgaacgccc aggttggcat
    20641 cacctcgccg ggctttattg gcgcggacgt ttcgcacctc aacctgcata aaactttctg
    20701 cattccgcac ggcggtggtg gtccgggtat gggaccgatc ggtgtgaaag cgcatctggc
    20761 accgtttgta ccgggtcata gcgtggtgca aatcgaaggc atgttaaccc gtcagggcgc
    20821 agtttctgcg gcaccgttcg gtagcgcctc tatcctgcca atcagctgga tgtacatccg
    20881 catgatgggc gcagaagggc tgaaaaaagc aagccaggtg gcaatcctca acgccaacta
    20941 tattgccagc cgcctgcagg atgccttccc ggtgctgtat accggtcgcg acggtcgcgt
    21001 ggcgcacgaa tgtattctcg atattcgccc gctgaaagaa gaaaccagca tcagcgagct
    21061 ggatattgcc aagcgcctga tcgactacgg tttccacgcg ccgacgatgt cgttcccggt
    21121 ggcgggtacg ctgatggttg aaccgactga atctgaaagc aaagtggaac tggatcgctt
    21181 tatcgacgcg atgctggcta tccgcgcaga aattgaccag gtgaaagccg gtgtctggcc
    21241 gctggaagat aacccgctgg tgaacgcgcc gcacattcag agcgaactgg tcgccgagtg
    21301 ggcgcatccg tacagccatg aagttgcggt attcccggca ggcgtggcag acaaatactg
    21361 gccgacggtg aaacgtctgg atgatgttta cggcgaccgt aacctgttct gctcctgcgt
    21421 accgattagc gaataccagt aattcactga ttcgactatt ttctaaaggc gcttcggcgc
    21481 ctttttagtc agatgacaaa gtacaaaagt gctcagacag tcccctcgcc cctttgggga
    21541 gagggttagg gtgaggggaa caggccggca ctggcgcgaa tatttaccct caccccggcc
    21601 ctctccctga aagggcgagg ggaaaagcgt gccaacattg aagattgagc cagtttgtta
    21661 gcaatctcaa agatacgtca acgaattaat ttttctcgga aaaacaaatg gctatagcac
    21721 ttgtgaccgg tggcagtcgc ggcatcgggc gggcaactgc attactgttg gcgaaagaag
    21781 agtatacggt ggcggttaat tatcaacaaa acctccatgc ggcgcaggaa gtggtgaact
    21841 taatcacgca agccggtggc aaggcattcg tgctccaggc ggatatcagc gacgaaaatc
    21901 aggtcatcgc aatgtttaca gcaatcgatc agcacgatga accgctagca gcgctggtca
    21961 ataacgccgg gatcttgttt acccagtgca ccgttgaaaa ccttaccgca gagcgaatca
    22021 accgagtact ttccaccaac gtgacgggat attttctctg ctgccgcgag gcggtaaaac
    22081 gcatggcgct taaaaatggt ggcagtggcg gcgctatcgt caatgtctct tcggtggcct
    22141 cacggttggg ttcgccaggg gaataggttg attacgcggc atcgaaaggg gcgattgata
    22201 cgttaaccac cggactatcg ctggaagtcg ccgcgcaggg gatccgcgtt aactgcgtgc
    22261 ggccagggtt tatttatacc gaaatgcacg ccagcggcgg cgagcttgga cgcgtcgatc
    22321 gcgttaagtc gaacatcccc atgcagcgtg gtggacaggc agaagaggtc gcgcaggcca
    22381 ttgtctggct actaagtgat aaagcctctt acgtcacggg aagttttatc gatttggcgg
    22441 gcgggaaata aaacagggaa gttgtctgac cggatgcaac aagtattgca tccggtactt
    22501 catcgactta aagcttctcg ccgttgctgg caatcacctc tttgtaccag ttaaagctct
    22561 tcttacgtga acgcgacata tcgccagtac cgtcgtcatg tttattcaca tagataaagc
    22621 cgtagcgttt gctgtactgc ccggtagtga atgacacgca gtcgatgcag ccccacggtg
    22681 tgtagcccat cagatccaca ccatcgtaag tcactgcttt tttcatctct tcaatatggg
    22741 cgcgcaggta gtcaatgcgg tagtcgtcgt tgatgctgcc atcttcttcc actttgtcgt
    22801 aagcgccaaa accgttttcg acaataaaca gcggcctctg ataacgctca tacagttcgc
    22861 aaagggcata gcgcagacct actggatcaa tctgccagca ccagtcagac gctttaacat
    22921 acgggttcgg tacgctgcct tcaaaaccag agatcgcatc gccggtgccg ccttcggcct
    22981 tcactgcgtt gatcatgtaa tagctgaaac caagataatc gcaggtgcct tcacgcagta
    23041 catccagatc gccatcttcc attttgatgt taaatccgcg acgctcccac tcgttcaaca
    23101 cataggacgg gtaatagccg cgtagctgca catcggtaaa gacgtagcgt tcgcgcatcg
    23161 actcctgagc gaacatcaca tcgtccgggt tacaggagta aggatagagc ggcaccatcg
    23221 ccagcataca gccgactttc atctccgggt taatgcgacg cgcggctttc actgctaggg
    23281 cgctggcgac aaactggtga tgcagcacct ggtacatcgt ctcttccggg ttgtcatgct
    23341 cggtatacac cacgccggag cagcagtaac caaacagcgg cgcacgccag ttacgctggt
    23401 tgtttatttc gttgaaggtc atccagtatt tgactttatg cttgtagcgt tcgaatacca
    23461 cttccgcgaa acggacaaag aaatcaacga ctttacggtt ggtccagcta ccgtattgct
    23521 gcaccagatg cagcggtatt tcaaagtggg agagggttat caccggttcg atgttgtatt
    23581 tcagcagttc atcgaacaga gagtcgtaaa acttcagccc ttcttcgttt ggctgagctt
    23641 catcaccttt tggaaaaata cgcgtccagg cgatggaagt ccgaaaacat ttgaagccca
    23701 tttcggcaaa tagcttgatg tcttccttgt agtgaccata aaaatcaacg gcttcatggt
    23761 ttggatagta ttttcctggc aagacttctt tggtgatttc gcgcggcacg ccgtgtgcgc
    23821 caccggtcag aacgtcacaa atgctcggtc ctttgccgcc tttgttccag ccgccttcga
    23881 cctgatgagc ggcaacggcg ccaccccata agaaatcttt cggtaaggtg agttttttca
    23941 ctatcatagg ctccagatga gtcaattctt acaggaaata gtaccatgca gccaaacgac
    24001 atcacttttt ctcaacgttt ccaggatgac attctggctg ggcgtaaaac catcaccatc
    24061 cgcgacgagt ctgaatcgca cttcaaaacg ggtgatgtgc ttcgtgtcgg acgttttgaa
    24121 gatgacggtt atttttgcac gattgaagtc accgcaacct caaccgtaac gctggatacg
    24181 ctgacggaaa aacatgcaga gcaggaaaat atgaccctga ctgaactgat aaaggtcatt
    24241 gccgacatct atcccggtca gacacagttt tatgtgattg aatttaaatg tctttaaatt
    24301 gaactcacgc ctgttagttg aaatttctca tttaatttca tgatttttag gagattagtc
    24361 tcaaatcaga atttatttta gctaacaggt gttcactgga actattctca gttacgctgg
    24421 agaggtatac atgtgcgtgt acgtttctcc ggagtaagtt atggttcaga agcccctcat
    24481 taagcaggga tattcgctgg cagaggaaat tgccaacagc gtcagtcacg gcattgggtt
    24541 ggtgtttggt atcgttgggc tggtgttgct actggttcag gcggtggatc tcaatgccag
    24601 cgccacagcg ataaccagct acagcctcta tggcggcagt atgatcctgc tgttcctcgc
    24661 ttcgacgctc tatcacgcca ttcctcatca acgggcaaaa atgtggctga agaaatttga
    24721 ccattgcgct atttacctgt tgattgccgg aacctacacg ccgtttttgc tggtggggct
    24781 ggattctccg ttagcgcgcg ggttgatgat tgttatctgg agcctggcat tgctggggat
    24841 tctgtttaaa ctgaccatcg cgcaccgatt caaaatttta tctctggtga cctatctggc
    24901 gatgggctgg ctgtcgctgg tggtaattta tgaaatggca gttaagctcg cggcgggcag
    24961 cgttacctta ctggcggtag gcggcgtggt ttattcgctc ggagtgattt tctatgtctg
    25021 caaacgcatt ccctacaatc atgccatctg gcacggcttc gtgctcggcg gcagtatgtg
    25081 ccactttctg gcgatctatt tgtatattgg gcaggcgtaa tgggtatgcc tttgtaggcc
    25141 tgataagacg cgccagcgtc gcatcaggcg ataaatgcct gatgcgatac agagatttac
    25201 tcttcgagtg aatacggcag cggctcgata tgcagtgtat tcgcatcgtc gcgtacgcgg
    25261 aagatgctgt ccggttccat atcgttattc atgacgacct gtaccacgac ctgaccatct
    25321 tccagtttta ccgcagccag caccgtaccg gtacgacgcc agttctcgcc cattttcagc
    25381 tctaagtctt caccagcttc cggcagtcgg ctggcgctac ctgccagcaa ccagagcgca
    25441 cgtttattgg caccacggaa ttttgctcgc gccaccatct cttgtccggt ataacagcct
    25501 ttcttaaaac tgataccgcc cagcgcctgg agattggtcg cctgtgggat aaactgcccg
    25561 ctgttcgcgg catcaatcac cgggaaaccc gcttcaatgt ttaatgccag ccactgttgg
    25621 ctattgttca gttccgcttc accgcgcagt ttatcggtca gcatattggc agtagcttca
    25681 tcggttacga tcaggaaacg ttctgccggg tgttcaaacc atagcaaagt ggtcgcgcct
    25741 tctttaacta cctgtttttc tctcgaaggt agttcactaa agagattggc gagcgcggcg
    25801 cgcgcctgaa aaccggcaac accaagcagc acacgctcgt cgtctgccgc gatggtcact
    25861 ttagagaaca ccgcatattt tttcagttca gtcagctgcg gttcacgcac gctgcgccgt
    25921 tcaatccacg caaagccatc gccgtcgcgg aacagacgta aattgctcca cattttacct
    25981 ttggcgtcgc aatgggcggc gagcaggtgc tgattttctg tcatctggct gacatctgct
    26041 gtcacctgac cctgcatata tttttcgctg tccgcgccag taatggtggc aagcgcccag
    26101 tcatcaagcg tcatcagcgt cagcggtaaa cgggcagaag ccgtaggctg acggggagga
    26161 aaaggtgtaa aagccataat agagtcctga gttgcttaac ggcgtattaa taactaatgg
    26221 taaaagagct atgaggcaat gcaagtgctt tcactacgcg atctgtgcct tctgcgaggc
    26281 gacttccaaa aaaatgaagc tcttgaattg ttgtgaatta atttcgcgat cgcgatggtg
    26341 tttactgata ttcccgtaaa agaagtcgaa aaacacgtta caatagccgg gtactggatt
    26401 ttcgtgagac aggaagaaga tggacattaa caacaaagcc cgcattcatt gggcatgccg
    26461 ccgtggtatg cgcgaactcg atatttcaat catgccgttt ttcgaacatg agtacgacag
    26521 cttaagcgat gacgaaaaac gcatctttat tcgtctgctg gaatgtgacg atccggacct
    26581 gtttaactgg ctgatgaatc acggtaaacc ggccgatgca gaactggaaa tgatggtccg
    26641 actcatccag acacggaacc gggaacgtgg tcctgtggca atctgatttg cgcgtctcct
    26701 ggcgcgcaca gtggctttcc ttgctgattc atgggctggt tgccgctgtt attttactca
    26761 tgccctggcc gctcagttac accccgttat ggatggtgtt actttcgctg gtggtatttg
    26821 attgcgttcg cagccagcgg cgtattaatg ctcgccaggg ggaaattcga ttgttgatgg
    26881 acgggcgttt gcgttggcaa gggcaggagt ggagcatcgt caaagcaccg tggatgatta
    26941 agagcggcat gatgctgcgt ttacgttctg atggcggtaa acggcaacat ttatggctgg
    27001 cagccgacag catggacgaa gctgaatggc gggatttacg gcggattttg ttgcaacaag
    27061 agacgcaaag ataaatatga cggcaaagca tatgcgctaa gccgtcagtg agatcaggcg
    27121 taatgctctg ccatttcgtt gaggatttgc tcgcaccagc tctgaatacg ctcgtcgcta
    27181 aggtcatact ggttagtttc atccagcgcc agacccacga acagttgccc gtcagcaatc
    27241 accggtttcg ggctggtaaa ttcatatcct tccgttggcc agtagccgac gaacttcacg
    27301 cctttggtcg agagtttgtc atgcagcata ccgagcgcat cgaggaacca ctcgccgtat
    27361 cccagttgat cgccaagccc atacagcgca acaattttac cttcaaggtt caggtcgtcg
    27421 agctgatccc agacggcttc ccagtcttcc tggatttcgc caaaatccca ggtcgggata
    27481 cccagaatga gcacatcgta ctgctccatt aatttcgggg agtcgtcctt gaggttatgt
    27541 aaggtcacca gttctgggcc gataatatcg cggatttttt ctgccgccat ttcggtgtaa
    27601 caggtgctgg aaccgtaaaa aagacccata ttcataacgt aagcttctcg attctgctgt
    27661 ggcttttgcg gctagtgtat cagaatcgct ttagtcacgg cacaataagg cataatggat
    27721 caaatgagtg accataaggg gcgcaagtga aacaggatct ggcacgcatc gagcagtttc
    27781 ttgatgctct gtggctggaa aaaaatctgg ctgaaaatac gttgaacgct taccgtcgcg
    27841 atctgtcaat gatggtggag tggttgcatc accgcgggtt gacgctggcg acggcgcaaa
    27901 gtgacgattt gcaggcatta ctggcagaac ggctagaggg cgggtataaa gccaccagct
    27961 cagcgcgttt gctgagtgca gtgcgccgat tgttccagta tctttatcgc gaaaagtttc
    28021 gtgaagacga tcccagtgcg catctcgctt caccgaaatt gccacagcgt ttgccaaaag
    28081 atttaagcga agcgcaggtc gaacgtttat tacaggcacc attaattgat cagccactgg
    28141 agctacgcga taaagccatg cttgaagtgt tgtatgctac cgggctgcgt gtctctgaac
    28201 tggtcgggct gacaatgagt gatatcagcc tacgtcaggg cgtagtgcgg gtcattggta
    28261 aaggcaacaa agagcgcctg gtgccgttag gtgaagaggc cgtttactgg ctggaaacct
    28321 atctggaaca tgggcgtccg tggctgttga atggtgtgtc aattgacgtg ttgtttccca
    28381 gccagcgtgc gcagcagatg acgcgacaga ccttctggca ccgcattaaa cattatgccg
    28441 tgctggcggg tatcgacagc gaaaagctgt caccgcatgt gttgcgtcac gcttttgcca
    28501 ctcatttatt aaatcatggt gcggatttac gcgtggtgca gatgctactg ggccacagcg
    28561 atctctccac cacgcaaatt tatacgcatg tcgctaccga gcgtctgcgg caacttcatc
    28621 aacagcatca cccgcgggcg tgatgtctga aaagaacggg aagatttatg aagaaaggtt
    28681 ttatgttgtt tactttgtta gcggcgtttt caggctttgc tcaggctgat gacgcggcaa
    28741 ttcaacaaac gttagccaaa atgggcatta aaagcagcga tattcagcca gcacccgtgg
    28801 ctggcatgaa gactgtgctg actaacagcg gtgtgttgta catcaccgat gacggtaaac
    28861 atatcattca ggggccaatg tatgacgtta gtggcgcggc tccggtcaat gtcaccaata
    28921 aaatgctgct aaagcagttg aatgcgcttg aaaaagagat gatcgtttat aaagcgccgc
    28981 aggaaaaaca cgtcatcacc gtgtttactg atattacctg tgggtactgc cacaaactgc
    29041 atgagcaaat ggcagactac aacgcgctgg ggatcaccgt gcgttatctt gctttcccgc
    29101 gccaggggct ggagagccag gctgagcaac aaatgaaagc catctggtgt gcgaaagata
    29161 aaaacaaagc gtttgatgat gtgatggctg gtaaagctgc cactccggca agttgcgata
    29221 tcgatattgc cgatcattac gcacttggcg tccagcttgg cgttagcggc actccggcaa
    29281 ttgtgctgag caatggcaca cttgttccgg gttaccagcc gccgaaagag atgaaagaat
    29341 tcctcgacga acaccaaaaa atgaccagcg gtaattaatt cgcgtgaaac aacagataca
    29401 acttcgtcgc cgtgaagtcg atgaaacggc agacttgccc gctgaattgc ctcccttgct
    29461 gcgccgttta tacgccagcc ggggagtacg cagtgcgcaa gaactggaac gcagtgttaa
    29521 aggtatgttg ccctggcagc aactaagcgg tgtcgaaaag gccgttgaga tcctttacaa
    29581 cgcttttcgc gaaggaacgc ggattattgt ggtcggtgat ttcgacgccg acggcgcgac
    29641 cagcacggct ctaagcgtgc tggcgatgcg ctcgcttggt tgcagcaata tcgactacct
    29701 ggtaccaaac cgtttcgaag acggttacgg cttaagcccg gaagtggtcg atcaggccca
    29761 tgcccgtggc gcgcagttaa ttgtcacggt ggataacggt atttcctccc atgcgggggt
    29821 tgaacacgct cgctcgttgg gcattccggt tattgttacc gatcaccatt tgccaggcga
    29881 aacattaccc gcggcggaag cgatcattaa ccctaacttg cgcgactgta atttcccgtc
    29941 gaaatcactg gcaggcgtgg gtgtggcgtt ttatctgatg ctggcgctgc gcaccttttt
    30001 gcgcgatcag ggctggtttg atgagcgcgg catcgcaatt cctaacctgg cagaactgct
    30061 ggatctggtc gcgctgggga cagtggcgga cgtcgtgccg ctggacgcta ataatcgcat
    30121 tctgacctgg caggggatga gtcgcatccg tgccggaaag tgccgtccag ggattaaagc
    30181 gctgctggaa gtggcaaacc gtgatgcaca aaaactcgcc gccagcgatt taggttttgc
    30241 gctggggcca cgtctcaatg ctgccgggcg actggacgat atgtccgtcg gtgtggcgct
    30301 gttgctgtgc gacaacatcg gcgaagcgcg cgtgctggca aatgaactcg atgcgctaaa
    30361 tcagacgcga aaagagatcg aacaaggaat gcagattgaa gccttgaccc tgtgcgagaa
    30421 actggagcga agtcgcgaca cgctacccgg cgggctggca atgtatcacc ccgaatggca
    30481 tcagggcgtt gtcggtattc tggcttcgcg catcaaagag cgttttcacc gtccggttat
    30541 cgcctttgcg ccagcaggtg atggtacgct gaaaggttcc ggtcgctcca ttcaggggct
    30601 gcatatgcgt gatgcgctgg agcgattaga cacactctac cccggtatga tgctgaagtt
    30661 tggcggtcat gcgatggcgg cgggtttgtc gctggaagag gataaattcg aactctttca
    30721 acaacggttt ggcgagctgg ttaccgagtg gctggaccct tcgctattgc aaggcgaagt
    30781 ggtgtcagac ggcccgttaa gcccggccga aatgaccatg gaagtggcgc agctgctgcg
    30841 cgatgctggc ccgtgggggc agatgttccc ggagccgctg tttgatggtc atttccgtct
    30901 gctgcaacag cggctggtgg gcgaacgtca tttgaaagtc atggtcgaac cggtcggcgg
    30961 cggtccgctg ctggatggta ttgcttttaa tgtcgatacc gccctctggc cggataacgg
    31021 cgtgcgcgaa gtgcaactgg cttacaagct cgatatcaac gagtttcgcg gcaaccgcag
    31081 cctgcaaatt atcatcgaca atatctggcc aatttagcgt catcttctct ataaaaaaga
    31141 gcgtggattg ggtacaatcc cgctcttatc accgcatttt gactagctca ataaaagaaa
    31201 tcagaccatg tttgaaatta atccggtaaa taatcgcatt caggacctca cggaacgctc
    31261 cgacgttctt agggggtatc tttgactacg acgccaagaa agagcgtctg gaagaagtaa
    31321 acgccgagct ggaacagccg gatgtctgga acgaacccga acgcgcacag gcgctgggta
    31381 aagagcgttc ctccctcgaa gccgttgtcg acaccctcga ccaaatgaaa caggggctgg
    31441 aagatgtttc tggtctgctg gaactggctg tagaagctga cgacgaagaa acctttaacg
    31501 aagccgttgc tgaactcgac gccctggaag aaaaactggc gcagcttgag ttccgccgta
    31561 tgttctctgg cgaatatgac agcgccgact gctacctcga tattcaggcg gggtctggcg
    31621 gtacggaagc acaggactgg gcgagcatgc ttgagcgtat gtatttgcgc tgggcagaat
    31681 cgcgtggttt caaaactgaa atcatcgaag agtcggaagg tgaagtggcg ggtattaaat
    31741 ccgtgacgat caaaatctcc ggcgattacg cttacggctg gctgcgtaca gaaactggcg
    31801 ttcaccgcct ggtgcgtaag agcccgtttg actccggcgg tcgtcgccac acgtcgttca
    31861 gctccgcgtt tgtttatccg gaagttgatg atgatattga tatcgaaatc aacccggcgg
    31921 atctgcgcat tgacgtttat cgcgcgtccg gcgcgggcgg tcagcacgtt aaccgtaccg
    31981 aatctgcggt gcgtattacc cacatcccga ccgggatcgt gacccagtgc cagaacgacc
    32041 gttcccagca caagaataaa gaccaggcca tgaagcagat gaaagcgaag ctttatgaac
    32101 tggagatgca gaagaaaaat gctgagaaac aggcgatgga agataacaaa tccgacatcg
    32161 gctggggcag ccagattcgt tcttatgtcc ttgatgactc ccgcattaaa gatctgcgca
    32221 ccggggttga aacccgcaat acgcaggccg tgctggacgg cagcctggat caatttatcg
    32281 aagcaagttt gaaagcaggg ttatgaggaa ccaacatgtc tgaacaacac gcacagggcg
    32341 ctgacgcggt agtcgatctt aacaatgaac tgaaaacgcg tcgtgagaag ctggcgaacc
    32401 tgcgtgagca gggaattgcc ttcccgaacg atttccgtcg cgatcatacc tctgaccaat
    32461 tgcacgcaga attcgacggt aaagagaacg aagaactgga agcgctgaac atcgaagtcg
    32521 ccgttgctgg ccgcatgatg acccgccgta ttatgggtaa agcctctttt gttaccctgc
    32581 aggacgttgg cggtcgtatt cagctgtacg ttgcccgtga cgatcttccg gaaggcgttt
    32641 acaacgagca gttcaaaaaa tgggatctcg gcgacatcct cggcgcgaaa ggtaaactgt
    32701 tcaaaaccaa aaccggcgaa ctgtctatcc actgcaccga gctgcgtctg ctgaccaaag
    32761 cactgcgtcc gctgccggac aaattccacg gcttgcagga tcaggaagcg cgctatcgtc
    32821 agcgttatct ggatctcatc tctaacgatg aatcccgcaa cacctttaaa gtgcgttcgc
    32881 agatcctctc tggcattcgc cagttcatgg taaatcgcgg ctttatggaa gttgaaacgc
    32941 cgatgatgca ggtgatccca ggcggtgccg ctgcgcgtcc gtttatcacc catcataacg
    33001 cgctggatct tgacatgtac ctgcgtatcg cgccggaact gtacctcaag cgtctggtgg
    33061 tcggtggctt cgagcgtgta ttcgaaatca accgtaactt ccgtaacgaa ggtatttccg
    33121 tacgtcataa cccagagttc accatgatgg aactctatat ggcttacgca gattacaaag
    33181 atctgatcga gctgaccgaa tcgctgttcc gtactctggc acaggatatt ctcggtaaga
    33241 cggaagtcac ctacggcgac gtgacgctgg acttcggtaa accgttcgaa aaactgacca
    33301 tgcgtgaagc gatcaagaaa tatcgcccgg aaaccgacat ggcggatctg gacaacttcg
    33361 actctgcaaa agcgattgct gaatctatcg gcatccacgt tgagaagagc tggggtctgg
    33421 gccgtatcgt taccgagatc ttcgaagaag tggcagaagc acatctgatt cagccgacct
    33481 tcattactaa atatccggca gaagtttctc cgctggcgcg tcgtaacgac attaacccgg
    33541 aaatcacaga ccgctttgag ttcttcatcg gtggtcgtga aatcggtaac ggctttagcg
    33601 agctgaatga cgcggaagat caggcgcagc gcttcctgga tcaggttgcc gcgaaagacg
    33661 caggtgacga cgaagcgatg ttctacgacg aagattacgt caccgcactg gaacacggct
    33721 taccgccgac agcaggtctg ggaattggta tcgaccgtat ggtaatgctg ttcaccaaca
    33781 gccataccat ccgcgacgtt attctgttcc cggcgatgcg tccagtaaaa taagcattac
    33841 gttatgctaa taaccccggc aaatgtcggg gttttttaat tgtgctgcgc gaaagcagac
    33901 aacaattttc ttgcttcact attggctgcc tgcattacca tccacggact gaacgcccac
    33961 ggcgtggcat caataccgtg taatacatct gctaaatcac accattgata atccatcact
    34021 tcatcatcgt tgatctgtaa cgcactgttg gtgcgtgcgg caaataccgg acacacttca
    34081 ttttccacaa tgccattcgg atcggtggcg cgatagcgaa agtcaggata gatagattca
    34141 ggaggcgtaa tttccacgcc aagctcataa cggcaacggc ggatcactgc gtcttcgttg
    34201 ctttctccca gttgtgggtg cccacaaacc gagttagtcc acacgccagg ccatgctttt
    34261 ttgctcagtg cgcggcgggt aactaataat tgccccttgg cattaaacag ccaactggag
    34321 aacgcgagat gtaagagggt gtctgccgtg tgtgcggcat acttttccag cgtacccgtg
    34381 ggaactccct gtgcattcaa taaaatgacg tgttccgttt gcataatttc tcacatgtaa
    34441 ttctgatcac tcatcattat agataattga agagtgaaat gattaaccaa gtgagcgttt
    34501 atcgacaacc gcccgttttg agcggatgcc gacaggtaaa aaccatttaa aaaagtagca
    34561 tcatatattt gaagcgactt tcaatggcca ttgcaacaca gtcagaaatg acaggaatag
    34621 agagagtaaa aagcaacagc agactgaata tagcgcgcat taaaagattt atattattct
    34681 ccatgctcat ttttcttcag cttgcgcatc agaagccaca tacgtactgt cctgactttt
    34741 aaatctttat cagagacgga ttccttcacc aggtcagcat gatgctgata gccattaaag
    34801 aagacgttca gcacaacggc gctaagggtt gcgagcataa tgccgctatg tagcagcggt
    34861 tgcagtacgg ccggtaactt agaaaagaaa tcgtgagaga gcgtcggagt catgccaacg
    34921 ccgagactga tcgccacaat atagaggttg taacggttgg tggtgtagtt acagcgcgac
    34981 agaattcgaa tccctgtcgc cagtaccatg ccgaacatca ctagaccagc gccgcccagc
    35041 acaaattgcg gaatggaggc taccagcacc gccatttttg gcaccatgcc aaacaggatt
    35101 aatataattc ccgaagaaat acacacccag cggctatgaa cgcgcgtcac gctaaccagg
    35161 ccaacgtttt gagaaaatga cgtgtggggg aagctgttaa acgtgccgcc tatcattgtc
    35221 cctacgccat cgacacgcag cccgcgaata atatcgtgtg aagagagttt acgaccgact
    35281 atttcaccca gtgccaggaa catccccatc gactcgataa acacgatgat taacacggca
    35341 gtcatggtca gaatggaaac gggatcgaaa atcggcatac caaacgacat cggggtgaca
    35401 atcgcaaacc acaaggcatc atgtagcccg gataaattga cttcattcat catccacgaa
    35461 agtaaaaagc caaatacaat ccccagtaat acggcgacgt tggacataaa tcctttcgca
    35521 tagcgagtaa tgagcaagat aaaaattaag acggcaaagg agatacctga ataaacggga
    35581 ttaccatatt gcggattccc tttacctccg gcggcccagt caatacccac ctgaatgatg
    35641 ctaagtccga cagaagtaat aaccacaccg gtaaccagtg gcgggaataa aggcatcaag
    35701 cgaccgataa gtggcgctaa taatgtggtg ataaaacccg cggcgatagt ggcaccaaat
    35761 atccccagca ggccaatatc cgggttcatc ccaatggcta tcattggtgt tacagcagca
    35821 aaggtcaccg acataatcac cggcaggcgg atccccataa agcggccgat accgatacat
    35881 tgcaataatg tgacgatccc gcagcaaaag agatccgagc taatgagcat cgcaatagct
    35941 tcttttgaga ggcccagtcg gtcaccaatc ataagaggaa cagcgactgc acctgcgtac
    36001 atgacaagga cgtgttgcag accgagtatg atcagctttc ctggtgataa tatgcggtca
    36061 acctcatcag ttgggcggtc ttgcccagaa gatgagagaa gttgggaatc tatggcgctc
    36121 atcagtgttt ctcctttcgc ccgccagagt aaaaagcaac cgtttacgtt aatgaaactg
    36181 tctaaacaca tgctcgtgga gtgccagttt ttgcgaggaa ggggagttag agacaggaat
    36241 tgcggaggca atcacataat cgtggttaaa ccacgaaaat gagaagagaa cctgacaatg
    36301 gaattatctg taattttctc atattgatat ttcattaatc attctggcat gtcgtactct
    36361 cgcatttaat cgtttttatc tggatagcac tcttttgatc ggcaatgttg aaataatgaa
    36421 agcggcatag attgaggggc aataatttga actcagtcgc aaaattataa ttaaaacggt
    36481 catgctacct aata
//
LOCUS       DAJVUJ010000020        36152 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.020, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000020 DAJVUJ010000000
VERSION     DAJVUJ010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36152
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.020"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(50..1699)
                     /gene="actP"
                     /locus_tag="OQQ48_000947"
     CDS             complement(50..1699)
                     /gene="actP"
                     /locus_tag="OQQ48_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HCS1951797.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGMLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGTMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(1696..2010)
                     /locus_tag="OQQ48_000948"
     CDS             complement(1696..2010)
                     /locus_tag="OQQ48_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HCS1951798.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(2142..4101)
                     /gene="acs"
                     /locus_tag="OQQ48_000949"
                     /pseudo
     CDS             complement(2142..4101)
                     /gene="acs"
                     /locus_tag="OQQ48_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
     gene            4494..5930
                     /gene="nrfA"
                     /locus_tag="OQQ48_000950"
     CDS             4494..5930
                     /gene="nrfA"
                     /locus_tag="OQQ48_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HCS1951799.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            5975..6541
                     /gene="nrfB"
                     /locus_tag="OQQ48_000951"
     CDS             5975..6541
                     /gene="nrfB"
                     /locus_tag="OQQ48_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HCS1951800.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6538..7209
                     /gene="nrfC"
                     /locus_tag="OQQ48_000952"
     CDS             6538..7209
                     /gene="nrfC"
                     /locus_tag="OQQ48_000952"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HCS1951801.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7206..8162
                     /gene="nrfD"
                     /locus_tag="OQQ48_000953"
     CDS             7206..8162
                     /gene="nrfD"
                     /locus_tag="OQQ48_000953"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HCS1951802.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLAQQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKSWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8182..9900
                     /locus_tag="OQQ48_000954"
     CDS             8182..9900
                     /locus_tag="OQQ48_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HCS1951803.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQCALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELMPERRFYEARRQ
                     QMMEPSIRWNGIHDWYVVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GRKRDE"
     gene            9893..10276
                     /gene="nrfF"
                     /locus_tag="OQQ48_000955"
     CDS             9893..10276
                     /gene="nrfF"
                     /locus_tag="OQQ48_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HCS1951804.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10273..10869
                     /gene="nrfG"
                     /locus_tag="OQQ48_000956"
     CDS             10273..10869
                     /gene="nrfG"
                     /locus_tag="OQQ48_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HCS1951805.1"
                     /translation="MKEPKIPVKMLTTLTILMVFLCVGSYLLSPKWQAVHAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11210..12523
                     /gene="gltP"
                     /locus_tag="OQQ48_000957"
     CDS             11210..12523
                     /gene="gltP"
                     /locus_tag="OQQ48_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HCS1951806.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(12714..13345)
                     /locus_tag="OQQ48_000958"
                     /pseudo
     CDS             complement(12714..13345)
                     /locus_tag="OQQ48_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
     gene            complement(13439..15586)
                     /gene="fdhF"
                     /locus_tag="OQQ48_000959"
     CDS             complement(13439..15586)
                     /gene="fdhF"
                     /locus_tag="OQQ48_000959"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(15167..15169),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HCS1951807.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIMANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGEQGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGSCNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(15784..17250)
                     /gene="mdtP"
                     /locus_tag="OQQ48_000960"
     CDS             complement(15784..17250)
                     /gene="mdtP"
                     /locus_tag="OQQ48_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HCS1951808.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPSLGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDSIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIHSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(17247..19298)
                     /gene="mdtO"
                     /locus_tag="OQQ48_000961"
     CDS             complement(17247..19298)
                     /gene="mdtO"
                     /locus_tag="OQQ48_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HCS1951809.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAISQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAVAEGQCWQSDWRISESEAVAARECNLENICQTLLQLGQMNPNTPPTPA
                     AKPPSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDLLHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGL
                     ATALSCSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(19298..20329)
                     /gene="mdtN"
                     /locus_tag="OQQ48_000962"
     CDS             complement(19298..20329)
                     /gene="mdtN"
                     /locus_tag="OQQ48_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HCS1951810.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDVLVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPNDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(20348..20623)
                     /locus_tag="OQQ48_000963"
     CDS             complement(20348..20623)
                     /locus_tag="OQQ48_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1951811.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILTLITRRIIQRANINLAFVGIIYTALFAVYAMLFWLAFF"
     gene            complement(20832..22817)
                     /gene="yjcS"
                     /locus_tag="OQQ48_000964"
     CDS             complement(20832..22817)
                     /gene="yjcS"
                     /locus_tag="OQQ48_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HCS1951812.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYYRGEDYKFDINAPAPETV
                     NPSLWRQSQLNGISGLFKVTDRMYQVRGQDISNITFIEGDTGIIVIDPLVTPPSAKAA
                     LDLYFQNRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSHRALYSYGLLLAHNPQGNIGNGLGVTLASGYPSIIAPNKTITKTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVRGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVISLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQPQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
     gene            23070..23387
                     /locus_tag="OQQ48_000965"
     CDS             23070..23387
                     /locus_tag="OQQ48_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HCS1951813.1"
                     /translation="MQPVFLFKKRTGAMGGHVAGTAGFCVAVPAFYSWSMSEVIDKDT
                     RSSERTDYPHYASEVALAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQ
                     RRN"
     gene            23456..23785
                     /gene="yjdP"
                     /locus_tag="OQQ48_000966"
     CDS             23456..23785
                     /gene="yjdP"
                     /locus_tag="OQQ48_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjdP"
                     /protein_id="HCS1951814.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(23932..24690)
                     /gene="phnP"
                     /locus_tag="OQQ48_000967"
     CDS             complement(23932..24690)
                     /gene="phnP"
                     /locus_tag="OQQ48_000967"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HCS1951815.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECVACARARRSPQYRRQPCSGVV
                     KFNDAITLIDAGLHDLADRWPPGSFQQFLLTHYHMDHVQGLFPLRWGVGDPIPVYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVIDCSHPPREDAPRSHCDLNTVLALNQVIRSPRV
                     ILTHISHQFDAWLMENALPSGFEAGFDGMEIGVA"
     gene            complement(24692..25141)
                     /gene="phnO"
                     /locus_tag="OQQ48_000968"
     CDS             complement(24692..25141)
                     /gene="phnO"
                     /locus_tag="OQQ48_000968"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HCS1951816.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PSMRYPSMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQACGLNVGSKL
                     LAWAEEEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            complement(25128..25685)
                     /gene="phnN"
                     /locus_tag="OQQ48_000969"
     CDS             complement(25128..25685)
                     /gene="phnN"
                     /locus_tag="OQQ48_000969"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HCS1951817.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEREFFTRAGQNLLALSWHANGLYYGVGIEIDLWLHAGFDVVVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLKNRGRENTSEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            complement(25685..26821)
                     /gene="phnM"
                     /locus_tag="OQQ48_000970"
     CDS             complement(25685..26821)
                     /gene="phnM"
                     /locus_tag="OQQ48_000970"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HCS1951818.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLNAVAIGDVR
                     DGGDQLENLEKMINTIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CRARQIALASHDDATHAHVAESHQLGSVIAEFTTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVRLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRQGNHIHIDHVWRQGKRVF"
     gene            complement(26818..27498)
                     /gene="phnL"
                     /locus_tag="OQQ48_000971"
     CDS             complement(26818..27498)
                     /gene="phnL"
                     /locus_tag="OQQ48_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HCS1951819.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLQRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIHEAKARGAAIVGIFHDEAVRNDVADR
                     LHPMGASS"
     gene            complement(27609..28367)
                     /gene="phnK"
                     /locus_tag="OQQ48_000972"
     CDS             complement(27609..28367)
                     /gene="phnK"
                     /locus_tag="OQQ48_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HCS1951820.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYESRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            complement(28364..29209)
                     /gene="phnJ"
                     /locus_tag="OQQ48_000973"
     CDS             complement(28364..29209)
                     /gene="phnJ"
                     /locus_tag="OQQ48_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HCS1951821.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKNQ"
     gene            complement(29202..30260)
                     /gene="phnI"
                     /locus_tag="OQQ48_000974"
     CDS             complement(29202..30260)
                     /gene="phnI"
                     /locus_tag="OQQ48_000974"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HCS1951822.1"
                     /translation="MYVAVKGGEKEIDAAHALQESRRRGNTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQSPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIMPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHYVD
                     FQAELELLKRLQQEQNHG"
     gene            complement(30260..30844)
                     /gene="phnH"
                     /locus_tag="OQQ48_000975"
     CDS             complement(30260..30844)
                     /gene="phnH"
                     /locus_tag="OQQ48_000975"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HCS1951823.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLSAPLSNDIVSQSLRFHTNAPLVNQSELATFAVTDEAIS
                     SEQLNALSSGTAVAPEAGATLILQVVSLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            complement(30841..31293)
                     /gene="phnG"
                     /locus_tag="OQQ48_000976"
     CDS             complement(30841..31293)
                     /gene="phnG"
                     /locus_tag="OQQ48_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HCS1951824.1"
                     /translation="MHADIATRQHWMSMLAHSQPAELAARLNALNITANYEVIRTAET
                     GLVQIQPRMGGTGERFFAGDATLTRAAVRLTDGPLGYSWVLGRDKQHAERCALIDALM
                     QQSRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            complement(31294..32019)
                     /gene="phnF"
                     /locus_tag="OQQ48_000977"
     CDS             complement(31294..32019)
                     /gene="phnF"
                     /locus_tag="OQQ48_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HCS1951825.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGIALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            complement(32040..32819)
                     /gene="phnE"
                     /locus_tag="OQQ48_000978"
     CDS             complement(32040..32819)
                     /gene="phnE"
                     /locus_tag="OQQ48_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HCS1951826.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFVADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            complement(32945..33961)
                     /gene="phnD"
                     /locus_tag="OQQ48_000979"
     CDS             complement(32945..33961)
                     /gene="phnD"
                     /locus_tag="OQQ48_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCS1951827.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQGVKSNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            complement(33986..34774)
                     /gene="phnC"
                     /locus_tag="OQQ48_000980"
     CDS             complement(33986..34774)
                     /gene="phnC"
                     /locus_tag="OQQ48_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HCS1951828.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHSEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRAHTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTREQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            complement(34907..35350)
                     /gene="yjdN"
                     /locus_tag="OQQ48_000981"
     CDS             complement(34907..35350)
                     /gene="yjdN"
                     /locus_tag="OQQ48_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HCS1951829.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            complement(35510..35845)
                     /locus_tag="OQQ48_000982"
     CDS             complement(35510..35845)
                     /locus_tag="OQQ48_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HCS1951830.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDTEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
BASE COUNT         7817 a         9693 c        10210 g         8432 t
ORIGIN      
        1 ctggagagga agagtattcc ccggtcaggc gaccggggaa ggggagagat taatgtgcgc
       61 ggccttgctc aacgccaaag ccggtctggg agcggataaa ctgcgcacgg aacagttcac
      121 gctcgcgcgc accttccgct gagttatcgg ttgccgagaa gaaccagatg ccgaggaatg
      181 ccacgctgat agagaacagc gccgggtatt catacgggaa gatggctttt tcgtgaccga
      241 ggatctgtac ccaaatagtc gggccgagga tcatcagcac cactgcggta atcaacccca
      301 gccagccgcc cagcatcgtg ccacgggtag tcagtttcga ccagtacatg gaaagcagaa
      361 tgatcgggaa gttacagctc gccgcgatgg caaacgcaag gcctaccata aaggcgatgt
      421 tctgattctc aaacagcatg ccgaggataa tcgcaatcac acccaaaatc aggacggtga
      481 ttttcgatac ccgcagttct tcacgttcgg tcgcaccttt tttgaagacg ttagcgtaca
      541 agtcatgcga aaccgcggat gcgcccgcca gcgtcagacc cgcaaccacc gcgaggatag
      601 tggcgaaagc aaccgctgaa ataaaaccga ggaacaggtt gccgcccact gcattcgcca
      661 ggtgaacggc cgccatgttg ttaccaccaa tcagatggcc cgccgcgtct ttatattccg
      721 gattcgcacc aaccagcatg atcgcgccga agccgataat aaaggtcaga atatagaagt
      781 agcccataaa cccggtggcg tagaacacgc tcttacgtgc ttcgcgggca tcgctgactg
      841 taaagaagcg catcagaatg tgcggcaagc ccgccgtacc aaacatcagt cccagaccca
      901 gagagagcgc ggagatcgga tctttcacca gcccgcccgg cttcatgatg tcgacacctt
      961 tcgggtgtac cgccatcgct tcgctgaaca gattattgaa gctaaagccg acgtgtttca
     1021 tcaccataaa ggccataaag ctggcaccga acagcaacag cacggcttta ataatttgca
     1081 cccaggtggt cgccagcatg ccgccgaaca ggacgtacat catcatcagc acgccgacca
     1141 gcaccaccgc aatgtgatag ttaaggccaa acagcagctc gatcagttta cctgcgccta
     1201 ccatctgggc gataaggtaa agcgccacca ccaccagaga accacaggcc gaaagaatac
     1261 gaatcggccc ttgtttcagg cggtaagagg ctacatcggc aaaggtgtag cgtcccaggt
     1321 tacgcagacg ttcggcaatc aggaacaaaa tgatcggcca gcccaccagg aagcccagcg
     1381 agtaaattaa gccgtcatag ccggaggtaa acaccagcgc ggagatcccc aaaaatgagg
     1441 cggcggacat atagtcaccg gcaatcgcca gcccgttctg gaagcctgtg atattgccgc
     1501 ctgccgtgta gtagtcgcta cgagaacgta cgcgttttga tgcccagtag gtaatgccga
     1561 gcgtaaacac gacgaaaatc aggaacataa taatcgcctg ccagttcgtt ggctggcgct
     1621 ctacggcccc gctaatagca tccgcggcgt tagctgcgaa agggagtgtg gcggcaagcg
     1681 ccgtcagaac tcttttcatg atgcttgtac ctcatgcagg acttcattat taagacggtc
     1741 gaattcgccg ttcgcccgcc agatgtagat accggtgaga acaaaggaga tcacaatcac
     1801 tccaacacca attggaatac cgcgtgtgac gctggtgttc ggattcagcg gcgtgcccag
     1861 ccagccgggc gcgaaggcga tcagtaaaat aaagccgata taaactgcca gcataataat
     1921 cgacaggatg gtggcaaacc gttgccgttt ttcgactaac tccctgaaat gcgcattgtc
     1981 ttctatccgc tgataaatag tgccattcat cacagattct ccagaggtaa tgtagggatt
     2041 gttttaattc ccgctccctt atgggagaag gttaacgctc gggtaaccct tgccgaatgt
     2101 aggccggata aggcgtttac gccgcatccg gcaattgtgg gttacgatgg catcgcgata
     2161 gcctgcttct cttcaagcag cttctcgact acgccaggat cggcaagcgt cgaggtatcg
     2221 cccaggttgc tggtatcgcc cgccgcaatt ttgcgcagaa tacggcgcat aattttgccg
     2281 gagcgggttt taggcaggga gtcggtccag tgcagcacgt ctggcgtcgc cagcgggcca
     2341 atctctttac gcacccagtt gcggacttct gcgtacagtt ctggtgacgg ttcctccccg
     2401 tgattaagcg tgacgtaggc gtagatcgcc tgacctttaa tattgtgcgg aatacctact
     2461 acggcggctt cggcaatctt cggatgcgcc accagcgccg actcaatctc tgccgtcccc
     2521 agacggtgac cggagacgtt cagcacgtcg tccacacgcc cggtgatcca gtaatagcca
     2581 tcttcatcgc gacgcgcgcc gtcgccgctg aaatacatat ttttgaaggt ggagaagtag
     2641 gtctgttcaa aacgttcgtg atcgccaaac agcgtacgcg cctgacccgg ccaggagtcg
     2701 gtgatcacca ggctgccttc ggtagcgcct tccagcgggt taccttcgtt atcgaccagc
     2761 gccggttgca cgccgaagaa cggacgtgtt gccgaaccgg ctttcagccc ggtagcgcca
     2821 ggcagtggcg tgatcatgaa accgccagtt tcggtctgcc accaggtatc gaccaccgga
     2881 catttctcgt tgccgatttt tttccagtac cactcccacg cttccgggtt aattggctcg
     2941 cccacggaac cgagaatgcg cagcgacgaa cggtcggtac cctcgatcgc tttatcgcct
     3001 tccgccatca gcgcgcggat cgccgtgggt gcggtataga gaatattgac ctgatgcttg
     3061 tccaccacct gcgccatacg ggcaggcgtc ggccagttgg gtacgccttc aaacatcagc
     3121 gtggtcgcac cgcaggccag cgggccgtac agcaagtaac tgtgtccggt cacccagccc
     3181 acatcggcgg tgcaccagta gatatcaccc ggatgataat caaagacata tttaaaggtc
     3241 agcgccgcgt acaccagata accgccggta gtatgcagca caccttttgg cttaccggta
     3301 gaaccggagg tgtagagaat aaacagcgga tcttcggcgt tcatctcttc cgcttggtgc
     3361 tgatcgctcg cttgctcaac caggtcgtgc caccacaggt cgcgcccttc ctgccagtca
     3421 attttcccgc cagtacgctt cagtaccacc acatgctcta cgctggtgac gttcgggttt
     3481 ttcagcgcgt catcaacgtt tttcttcagc ggaatactgc cgcccggcac gcacaccttc
     3541 gtcggaagtg atcaccagtc gtgagttgga atcaataatg cgcccggcaa cggcttccgg
     3601 cgagaagccg ccgaaaatca ccgaatgcac cgcgccaatg cgggcgcagg ccagcatcgc
     3661 aaccgcggct tccggcacca tcggcatata aatcgccacc acatcacctt ttttaatgcc
     3721 cagctcgagc agggtattgg cgaagcggca gacgtcgcgg tgcagctctt tatagctgat
     3781 atgtttgctc tggctgacgt cgtcgccttc ccagatgatg gcggtacgat cgccgttttc
     3841 ttgcagatgg cggtcaaggc agtttgccgc cagattcagc gtgccgtcct cgtaccattt
     3901 aatggacaca ttaccggggg caaaggaggt gtttttcacc ttctggtaag gtttgatcca
     3961 gtcaagaatt tttccctgtt cgccccagaa ggtatcaggt acgttaatag attgttgata
     4021 cattgcctcg tactgctgag ggtttatcag gcaacggtct gcgatgttgg caggaatggt
     4081 gtgtttgtga atttggctca tgcttttgtt ctccttgtag aatgttaata atatgtgaca
     4141 taagcgttaa atgtaggggt attggcagtt ttgtttagta tttgggcgac agatcacgca
     4201 aaagtagaat tgtgcaaata aacggcaggg taatttttga aggtcagcaa caaaagttga
     4261 ttaattcttt gaggaacatg cagttatgca tgctgttgaa aaagaggaag atactgacta
     4321 actctaaagt ggtattttac atgcacttac aattgattaa agacaacatt cacagtgtgg
     4381 ttatttgtta cacatagggg cgagcaatgt catgacagtg taggtgcggt tactgtcgtg
     4441 aaaaacaata aaaaccgcca ttgcaacaat ggcgcaattc ggatgaagcc cctatgacaa
     4501 ggataaaaat aaacgcacgc cgtatcttca gcttattgat tccttttttc tttttcactt
     4561 ctgttcacgc tgaacaaacg gcagctcccg caaaacctgt aactgtggaa gcgaagaatg
     4621 aaacctttgc cccgcagcat cccgatcaat atctctcctg gaaagccacc tcggaacagt
     4681 cagagcgtgt tgacgccctg gcggaagatc cacggctggt gatcctgtgg gcggggtatc
     4741 ccttctcgcg cgattacaac aagccgcgtg gacatgcctt tgctgtgacc gatgtgcgtg
     4801 aaaccctgcg taccggtgcg ccgaaaaatg ctgaagatgg tccgctaccg atggcgtgct
     4861 ggagttgtaa aagcccggat gtggcgcgtc tgatccagaa agacggcgaa gatggctact
     4921 tccacggtaa gtgggcgcgc ggcggcccgg aaatcgtcaa caacttaggt tgtgccgact
     4981 gccataacac cgcctcacca gagttcgcca aaggcaaacc ggagttaacc ctttcccgtc
     5041 cgtatgcggc tcgcgcgatg gaagccattg gtaaaccttt tgagaaagcc ggacgtttcg
     5101 accagcaatc gatggtttgc ggtcagtgcc atgtggagta ttacttcgac ggcaaaaaca
     5161 aagcggttaa attcccgtgg gatgacggca tgaaagtcga aaatatggag cagtattacg
     5221 acaaaattgc cttctctgac tggactaact ccctgtcgaa aacgccaatg ctgaaagcgc
     5281 agcacccgga atatgaaacc tggacagcgg gcattcacgg taaaaacaac gtgacctgta
     5341 tcgactgcca tatgccaaaa gtgcagaacg ccgaaggcaa actctacacc gaccataaaa
     5401 ttggtaatcc gtttgataac ttcgcccaga cttgtgcgaa ctgccatacc caggacaaag
     5461 ctgccttgca aaaagtggtc gcggaacgta agcagtcgat taacgacctg aaaatcaagg
     5521 ttgaagatca actggttcac gctcacttcg aagcgaaagc agcgctggat gcaggcgcga
     5581 cggaagctga aatgaagcca attcaggacg atatccgtca tgcccagtgg cgctgggatc
     5641 tggcgatcgc ttcccacggc attcatatgc acgcaccgga agaaggttta cggatgctcg
     5701 gtacggcgat ggataaagcg gcggatgcac gcaccaaact ggcgcgcctg ctggcgacca
     5761 aaggcatcac ccatgaaatc cagatcccgg atatctcaac caaagagaaa gcccagcagg
     5821 ccattggcct gaacatggaa caaatcaagg ccgagaagca ggacttcatc aaaacggtga
     5881 tcccgcagtg ggaagaacag gcacgtaaaa acggtctgtt aagccaataa ccccgttccg
     5941 cctcgcaagg ggcggaaaac acaatggagt gaatatgagc gtattacgtt cgttattaac
     6001 tgctggggtg ctggcgtcgg gcctgttgtg gagcctgaac ggaattaccg ctacccctgc
     6061 ggcgcaggca tctggtgatc gctacgaagt tacccagcag cgtaacccgg atgccgcctg
     6121 tctggactgt cataaaccag ataccgaagg tatgcatggc aaacatgctt ccgttatcaa
     6181 cccgaataac aaattgccgg tcacctgcac caactgtcac ggccagccat caccgcaaca
     6241 ccgcgaaggg gtgaaagatg tgatgcgctt taacgagccg atgtacaagg tcggagagca
     6301 gaacagcgtc tgtatgtcct gtcatctgcc ggaacagttg caaaaagcgt tctggccgca
     6361 cgatgtccac gtaaccaaag tggcgtgcgc cagctgccat tccctgcatc cacagcaaga
     6421 tacgatgcag acgttaagtg acaaaggacg gattaagatt tgcgtcgatt gccacagcga
     6481 tcagcgcacc aatccgaact ttaacccggc gtctgttccg ttgcttaagg agcagccatg
     6541 acctggtctc gtcgccagtt tctcaccggc gtcggcgtgc tggcagccgt cagcggcacc
     6601 gcagggcgcg tggtggcgaa aacgttgaat atcaatgggg tgcgttacgg catggtgcat
     6661 gacgagtcgt tatgcatcgg ctgtaccgcc tgtatggatg cttgtcgaga agtgaacaag
     6721 gtgccggaag gcgtctcgcg cctgacgatt attcgtagcg agccgcaggg cgaatttcct
     6781 gatgtgaaat atcgcttctt ccgtaagtct tgccagcact gcgatcatgc gccgtgcgtt
     6841 gacgtctgcc cgaccggtgc gtcttttcgt gatgccgcta gcgggattgt cgatgttaat
     6901 cctgatctct gcgtcggttg tcagtactgc atcgccgcct gtccgtaccg cgtgcgcttt
     6961 atccatccgg tcacgaagac ggcggacaaa tgcgatttct gccgtaaaac caatttgcag
     7021 gccggtaagc tgcccgcgtg cgttgaggct tgcccgacga aggcgctgac gtttggcaat
     7081 ctggacgatc ctaacagtga gatttcgcaa ctgctgcgcc agaagcctac ttaccgctac
     7141 aagctggcgc tgggaaccaa accgaagctg taccgcgtac cgtttaaata cggggaggtg
     7201 agccaatgac gcagacttcc gcatttcatt ttgaatcgct ggtgtgggac tggccgattg
     7261 ccatctacct gtttttgatt ggtatttctg ccggtctggt gacgctggcc gtgctgttac
     7321 gtcgcttcta cccgcaggcg ggcggtgcag acagtacgtt gctgcgcacc acgctgattg
     7381 tcgggccggg cgcggtgatc ctcggtctgt tgatcctcgt cttccacctg acaagaccgt
     7441 ggaccttctg gaagctgatg ttccactaca gttttacctc ggtgatgtcg atgggggtga
     7501 tgctgtttca gctttacatg gtggtgctgg tgctgtggct ggcgaaaatc tttgaacatg
     7561 atttgcttgc ccagcaacaa cgctggttgc cgaagctggg gatcgtgcaa aaggttctga
     7621 gcctgctgac gcccgttcat cgcggactgg aaacgctgat gctggtgttg gcggtgctgc
     7681 tgggggctta taccggcttt ctgctgtcgg cgctgaaatc gtatccgttc ctcaataacc
     7741 cgatcctgcc ggtgctgttc ctcttctccg gcatctcgtc cggtgcggcg gtggcgctga
     7801 tcgccatggc gatacgccaa cgcagtaacc cgcattccac ggaagcgcac tttgttcacc
     7861 ggatggaaat ccccgtggta tggggtgaaa tcttcctgct ggtggcgttt tttgtcggtc
     7921 tggcgctggg cgatgacggt aaagttcgtg cgctggtggc ggcattaggt ggcggtttct
     7981 ggacgtggtg gttctggctt ggtgtcgccg ggctggggct gattgtgcca atgttgctca
     8041 aatcgtgggt caatcgcagt tccggcattc ctgccgtgct ggcggcgtgt ggggccagtc
     8101 tggtcggcgt gttgatgctg cgctttttca ttctctacgc cggacagttg acggtggcgt
     8161 aagccagaaa agaggtggtt tctggacgta ttccttcctg aagtcggttt tctggcgttg
     8221 ttgttaagtc tcggggtcaa cgtgttgacc ccgttgacgg cctttgcggg agtgcggttg
     8281 cgctggcctg ccatgatgcg actcacttgc atcggcattc tggcgcagtg cgcgctcctg
     8341 ctgctcgcct ttggcgtact gacgtattgt tttctcatca gcgatttctc ggtcatttat
     8401 gtcgcccaac atagctacag cctgctgtcg tgggaactca aactggcggc ggtgtggggc
     8461 ggtcatgaag gttcgctgct gctttgggtg ctgctgcttt ccgcctggag cgcgctgttt
     8521 gcctggcatt atcggcagca aaccgatccg ctatttccgc tgacgctagc cgttttatct
     8581 ctcatgctcg ccgcactgct actgtttgtg gtgctgtggt ccgatccctt cgtgcggata
     8641 tttccaccag caatcgaagg ccgcgatctc aatccgatgc tgcaacatcc cggtcttatc
     8701 tttcatccac cgctgcttta ccttggctat ggcggtttga tggtagcggc gagcgtggcg
     8761 ctggcgagtc tactgcgcgg cgagtttgat gcggccagcg cccgaatttg ctggcgctgg
     8821 gcgttacctg gctggagtgc attaacggcg gggatcatcc tcggttcctg gtgggcctac
     8881 tgcgaactcg gctggggcgg ctggtggttc tgggatccgg tggaaaatgc ctctttatta
     8941 ccctggcttt ctgccactgc gctgctgcac agtttatccc tgacacgcca gcgggggatt
     9001 tttcgccact ggtcgctgtt actggcgata gttactctga tgctgtcgct gctgggcacc
     9061 ttaattgtcc gttctggcat tctggtttcg gttcatgcgt tcgcgctgga taacgtccgc
     9121 gccgtgccgt tgttcagcct gtttgcactg attagccttg cgtctctggc tctgtatggc
     9181 tggcgagcgc gggacggtgg cccggcggtg cgtttttcgg ggttatcgcg ggaaatgtta
     9241 atcctcgcta cgctgttgct gttttgcgca gtgctactga tcgtgctggt gggaacgctt
     9301 tatccgatga tttacggtct gctgggctgg ggacgcctct ccgttggcgc gccgtatttt
     9361 aaccgcgcga cgttaccgtt tggtctgttg atgctggtgg tgattgtgct ggcgacgttt
     9421 gtctctggca aacgcgtgca gcttccggcg ctggtagctc atgcgggcgt gctgttattt
     9481 gccgctggga tcgtggtttc cagcgtcagc cgtcaggaga tcagcctgaa tttacagccg
     9541 ggtcagcagg tgacgctggc aggatacacc ttccgttttg agcgcctcga tctgcaagcc
     9601 aaaggcaatt acaccagcga aaaagcgata gtggcactgt ttgaccatca gcaacgcatt
     9661 ggtgaactga tgccggagcg gcgtttttac gaagcacgtc gtcagcaaat gatggaaccg
     9721 tcaattcgct ggaacggcat ccatgactgg tatgtggtca tgggtgaaaa aaccggagcg
     9781 gatcgttacg cttttcgctt gtatgtacaa agcggtgtgc gctggatctg ggggggagga
     9841 ttgttgatga ttgcgggcgc attgttaagc ggatggcggg ggaggaagcg cgatgaataa
     9901 aggacttctc acgctactgc tgttatttac ctgttttgcg cgcgctcagg tcgtagacac
     9961 ctggcaattc gccaatccgc aacaacagca acaggcgtta aatattgcca gccagttacg
    10021 ttgtccgcag tgccagaatc aaaacttact ggaatccaac gcgccggtgg cagtcagtat
    10081 gcgccatcag gtttacagca tggtggcgga ggggaagaac gaagtcgaaa tcattggctg
    10141 gatgaccgaa cgctacggag attttgttcg ctataaccca ccgttaacgg gtcagacgct
    10201 ggtgttgtgg gcgctgccag tggtgttgtt actgctgatg gcactgatcc tctggcgagt
    10261 gagggcgaag cgatgaaaga gcccaaaata ccggtgaaaa tgctgacaac actcacgatt
    10321 ttgatggtat ttctctgtgt cggcagttat ctgttaagtc caaaatggca ggcggtacat
    10381 gcggaatatc agcgtcagcg cgatccgcta catcagtttg ccagccagca aaccccggaa
    10441 gcgcagcttc aggcattgca ggataaaatc cgcgctaatc cacaaaacag cgaacaatgg
    10501 gcgttactgg gcgagtacta tctgtggcaa aacgattaca gcaattcgct gctggcgtac
    10561 cgtcaggcgt tgcaactacg tggtgagaac gctgaactgt atgcggcgct ggcgacggtg
    10621 ctttattacc aggccagcca gcatatgacc gcccagactc gcgcaatgat cgacaaagcc
    10681 ctcgcgctgg actctaacga aatcaccgct ctgatgctgc tggcttccga tgcgtttatg
    10741 caggcgaatt acgcgcaagc catcgaacta tggcaaaaag tgatggatct caactcaccc
    10801 cggatcaacc ggacacagct ggtcgagtcg attaatatgg cgaaattgtt gcagcggaga
    10861 tcggattaat catctggtga tgaagattat gttattgagc tgcataaaaa ataatcgaat
    10921 gaacatatgc caaaaataat cactaatcag tattattgca gattaaacaa ataaaaatct
    10981 ttccataaca aatggttatt cattaatcct gacataaact cgcgttatta tgcattaatg
    11041 cagcgaaaag ctctgttgtt aaagggttgc gcaacatacc gcgcaaatga tactgatcat
    11101 aagcgttaaa aaaatctaca aaccaacgca acacaattca tgcctggcag tatgtcacgt
    11161 tctcgcgttt ctgaacgggg aacggcgctc cattgaggaa gttattctga tgaaaaatat
    11221 aaaattcagc ctggcctggc agattctgtt tgctatggtg ctgggcattc tcctgggaag
    11281 ctacctgcac taccatagcg acagccgcga ctggctggtc gtcaatttgc tctctccggc
    11341 gggtgatatc ttcatccatc tgattaaaat gattgttgtg ccgattgtga tctccacgct
    11401 ggtggtgggt atcgcgggtg tgggtgatgc caaacagctc gggcgtattg gcgcgaaaac
    11461 cattatttac ttcgaggtga tcaccaccgt cgccatcatt ttggggatca ctctggcgaa
    11521 cgtcttccag cccggtgccg gggtggatat gtcgcagctg gcgaccgtcg atatctcgaa
    11581 atatcagagc actacggaag cggtacaaag cagttcccac ggcattatgg gcacgatttt
    11641 gtcgctggtg ccgacgaaca ttgtggcgtc gatggcgaaa ggcgaaatgc tgccgatcat
    11701 cttcttctcg gtgctgtttg gtctggggct ttcctccctg cccgcgacgc atcgtgaacc
    11761 gctggtgacc gtgttccgct ccatctctga aaccatgttt aaagtgactc acatggtgat
    11821 gcgttatgca ccggtgggtg tgtttgcgct gattgcggtg acggtggcta actttggttt
    11881 ctcttctctg tggccactgg cgaaactggt gctgctggtg catttcgcca ttctgttctt
    11941 cgcgctggta gtgctgggaa ttgtggcgcg cctgtgcggg ttaagcgtct ggatcctgat
    12001 tcgtattctg aaagatgagc tgattctggc gtactccact gccagctctg aaagcgtact
    12061 gccgcgaatt attgagaaga tggaagccta cggagcaccg gcgtcgatca ccagcttcgt
    12121 ggtgccgacc ggttactctt ttaaccttga tggttcgacg ctgtatcaaa gtattgccgc
    12181 tattttcatc gctcagctgt atggcattga cctgtccatc tggcaggaaa tcattctggt
    12241 gctgacgctg atggtgacct cgaaggggat tgctggcgtg ccgggcgtgt cgtttgtggt
    12301 gttgctggca acgctgggta gcgtaggtat tccgctggaa ggtctggcgt ttattgctgg
    12361 tgttgaccgt atcctcgaca tggcgcgtac tgcgctgaac gtggtgggta atgcgctggc
    12421 ggtgctggtg attgccaagt gggaacacaa atttgaccgc aaaaaggcgc tggcttatga
    12481 gcgtgaagtg ctgggcaaat ttgataaaac tgcggatcaa taattgaaga ttgccggggt
    12541 tatccacccg gcaatgtgtg gatgcctgat gcgacgctgg tgcgtcttat caggcctacg
    12601 ccagacagcg caatagcctg atttagcgtg attttgtagg tcggataagg cgtttacgcc
    12661 gcatccgaca tcaacgcctg atgcgacgct taacgcgtct tatcaggcct accttaaccg
    12721 ttggttaatt tttcaaactc ttcacacccg gtatcaaacc cttccataca gctcaggttc
    12781 aaccagtgca gcgccttttg tttgttcttc tcgataaaac cttcttcacc gtttaagaac
    12841 atcattcccg cccagtactc ggaataaccg gttcgggaaa ttgcagagct gcgtttgaaa
    12901 tactaggttg ccttgtcatc gacggcagag tcgttctcca gatcttccga ggcttttcta
    12961 acagcgaaat cgcctttgga taatccggtt tacccgcttg agtatttacc agaatatgcg
    13021 ccagcgttac ttcaccttct ttactgcccg cttgcgccgc tttctctgca agaacttttg
    13081 cttgtggata gtccagactg cccggattgg tgattttgat ctgcgccaac agcgcgcagg
    13141 catcagcatc accgctgtcg gcggctttct gtgcccaata ttcggctttg ctcaaatcgc
    13201 cggagctaaa ccagctgtcg gcaagaaaat attgtgcgcg tcggtccccg gcctctgctg
    13261 cctttaaata ctggctgcca ggctcggagt cgttggcgtg ggcaaagaat gtcaaaaaca
    13321 acatcaatgc gataattttt ttcattttga taatacgatt gggtagggag taaccagtat
    13381 aaagagttcg aatggataaa aaaacagcct ccgaaaggag gctgtagaaa ggactgtatt
    13441 acgccagtgc cgcttcgcgc aggcgagttt tcaacttgtt gtactcgtca atcacgtact
    13501 gctcggcggc gcgctgatcg gcgatcggct cgacgcgcac ggcgcagtat ttgtattccg
    13561 gcgttttcgt aatcgggctt aagttttcgg taaccagctc gttacaggaa ccaatccacc
    13621 actggtaggt catgtaaatc gcccctttgt tcggacgatc gctgacctgc gcacgggtga
    13681 taattttgcc tttacgcgag tgcacccaaa ccaatgcctc atcttcaata cccagacgtt
    13741 tggcgtcttc ggtattgatt tgtgcgtagc caggttcatc agccagcgcc gccagtgccg
    13801 cacagttacc ggtcatcgaa cggcaagagt agtgaccaac ttcacgcacc gttgacagta
    13861 ccatcgggta ctcgtcggtg agtttgtcga ttggcgctac ccagtcgcag gtgaagaact
    13921 gcgccagacc gttcggggta tcaaacttct ctttaaacag ataagaagtc ccctgatcgg
    13981 catctgaagt atcgcggcaa ggccactgaa tgaagccctg ttcgcccatt ttctcgtaag
    14041 tcgcaccgta gaaatccggg cacagatgac gcaactcatc ccagatctcc tgggtgttgt
    14101 tgtagtgcat cggataaccc atacgggtgg cgatttcact gatgatttgc cagtccgttt
    14161 tcagatccca tttcggttca actgctttga agaaacgctg gaagccacgg tcagccgcag
    14221 taaacacgcc ttcatgctcg ccccacgacg ttgacggcaa aatgacatcc gccgccgacg
    14281 cggttttggt cataaagatg tcctgaacga tgaccagttc cagatcttca aaggctttac
    14341 gtactgccga cagctccgca tcagtttgca gcggatcttc gcccataatg tacgcggcac
    14401 gcacttcgcc atgcgctgcg cggtgcggca gctcgctgat gcgatagccg gtatgagcag
    14461 gcaggctttc cacgccccag gctttggcga atttctcgcg gttagccgga tctttcacgt
    14521 actggtatcc cggatacgta tccggcagcg cgcccatatc gcaggcaccc tgcacgttgt
    14581 tctgaccacg aaccgggttt acacccgcat gcggcttacc gaggttaccg gtcagcatcg
    14641 cgaggctggt cagagaacgc acggtttcca cgccctggta gaactgggtt acacccatgc
    14701 cccacaggat ggcggcgctt ttcgcctggg catacatccg tgccgcctga cgaatctcac
    14761 tggcgctgac gccggtgata tcttcaaccg actccggcgt gtagccttca acgattttac
    14821 gatactcttc aaagccttct gtacgggaag cgacgaacgc tttgtcgtac agattttctt
    14881 caataatgac atggcccatc gcattcaaca gcgcgatgtt cgagccgttt ttcagtgcaa
    14941 tgtgcatgtc agcaatgcgc gcggtttcaa ttttgcgcgg atcgcagacg ataattttcg
    15001 ccccgttacg tttagcgtta attacgtgat tcgccatgat tgggtgggaa tccgccgggt
    15061 tgtacccgaa aacgaacact aaatcggtat tatcaatttc gttaatagca ttgctcattg
    15121 cgccattacc gaccgattgg tgcagacctg caaccgatgg gccgtgtcag acacgagcgc
    15181 agcagtcaac gttattggta ccaataacgg cgcgcgcaaa tttttgcatt acatagttgg
    15241 tttcgttacc cgtaccacgc gaggagccgg tcgtctggat ggcatccgga ccgtacttct
    15301 ctttgatggc gctcaggcgc tcggcaacgt aattcagtgc ctcatcccag gaaacaggtt
    15361 cgagtttgcc gccacgctgg cgacggatca tgggggtttt caggcgcggg gtcaggatct
    15421 gggtatcgtt aatgaagtcc cagccataat aacccttcag acacagggta ccctggttgg
    15481 ttttcccctg cgctgcctcc gcccggacga ttttgccgtt atcgacgacc aggttgattt
    15541 tgcaacctga tgcgcaatag gggcaaaccg tgacgacttt tttcatcggt ctcgctccag
    15601 ttaatcaaat cacgcatacg cgctctcgac tacagtatgc atcttttatg ccacatttta
    15661 tttggggtca ttccctgata ttacgggcac tatttattca aaactctgac gaaaaacagg
    15721 ctgtcgtcag ttttgacgtg acgaaacgaa ataccgcgtg acagccatca cgcggcagac
    15781 attttatttt ttctcgacga cgggagccgc ctgatacccg ccgcccagcg atttcatcaa
    15841 ctgaatgctg tggatcaccc ggcggctgtc cagcatcagt aatgacatct cttcggcaag
    15901 cactggcaac cgggcttcgg tggcctgtaa gcggctggtt aagccgcgct gataggcggc
    15961 ctcggcagcg cgctgggtga agcgcgttgc ttccacgcgt tcagcctgca tttctcgttc
    16021 gtcgttgagc gtttgcagac gcgtgccgtt gacggcaacg tcacgcaccg cgttcagtac
    16081 tgactggttg taacgttcaa tcatcatgtt gctggcggcg cgagtgcctt cgagattggc
    16141 attcaaccgt ccaccgtcaa acagcggcaa tttcagaccc gggatgaagt tgaactggcg
    16201 actggttttt ttgaataagg tatccaggtg gatggagtcc agaccgaaaa acgctttgat
    16261 atcaaagctc gggtagaaca gtgcccgcgc ggaatccacc tgatctaatg acgcctgaac
    16321 ataccagcgc atggcttgca gatccgggcg tctggcgagc aactcataag agagtgtcgc
    16381 cggaatgccg gtctggactc gtggtaatgc caccggtttg atctccggca tatcgctggc
    16441 ccccgcgcca atcaatgcac gcagagattc tcgcgtttcg gtgatttgcc ctttgacggc
    16501 agcaatttgt ttatcgaccg ccagaatctg tgcccgcgcg ccgtggaaag gcacttgtgc
    16561 ttccagaccg tgcgccactt tactctggtg cgctttcacc gcgtaatcaa tcacatcgcg
    16621 agtttgttct aacagatcga gcatctgata gctggcctgc atactgtaat aaagctgcgc
    16681 tacgcccgtg gtcagcgata gctctactgc tgcggtttct gccagcgcgg cattatgcgc
    16741 gccaatggcg gcggcaaccg ctgagcgatg cacaccccac aaatcaagat ccagtccggc
    16801 aaacaaacct actgtggctt ccgtatagta cggcccgtcc atacccagtg agggcgcatc
    16861 catcgcataa gggcttaaaa agccgttcgc cgagacgcgt tggcggttga gcatccctaa
    16921 cgctgcgacc tgtaattgtg aaccggcatc taacaaatcg gcctgggact gcgctttttc
    16981 ttcccgcagt ttcgcttcgg cgagggtgtg tgaaccactt agcgtccgtt ggatcagcgc
    17041 atccagctgc gggtcattga gttgtttcca ccactgcgcc tgcggccagc cggagctggc
    17101 aagatgaata tcgtcggcca gtttgatttg ttccggtttg agctgttgat gaggtgcaga
    17161 atccttacgt accagggcac agccggaaat cagcgtcgtg ctgccgagaa tgctgcacaa
    17221 cagcagacgt gaaagttgac gattgatcat tgcgtggctc cttgtgcctg ttccgtggcg
    17281 ggcgttaatg ccggggctgt ccagtctggc aagcgggcga aaagctggca gacgtgttgc
    17341 tcctgtttta ataaggtggg caggatggcc tgcgaggcgg gtgtctcttc aagcgttatt
    17401 tgaggagaac agctgagtgc ggttgccaga ccggcagcgt atttttccag ggcgtcggca
    17461 aactgacctg ctctgtccgg ctgtagtgcg ttatccagcg cctgcgccga gttccaggtg
    17521 gcatcccagg cgtgaagaag atcgcggccc tgacgaataa ccgtttgcga acgttcaatc
    17581 agtaaggcgc gttcttcgct gtccagttga cgctccagcg ccacgcgttg gcacatctct
    17641 tcacaggcat taaacgccgc atgcagacct atacgaattt gcagataagt gcgcagagcc
    17701 gtgacttcct gctggcgtgg gatccgcatt actttactta gcatacccag cgcaccggct
    17761 agtttttgcg gcagagtgcg cgcttcactt tcaggccaga caaaggtgta aatcaccgcg
    17821 gacaccacgg taccaatgat gatacccagg gcgcgatcgc gaatttccac caggtcgtac
    17881 actgggccaa aaacgttttc gagcgtggcg agcgcgaagg tgacgaccat ctgtgtgccg
    17941 atataagaag agcgttcaga gctggtggca atccatgcgc ccaacaggaa aatcggtgcc
    18001 agcacaaaca gcaattcgac aatattgtcc agccagggca tgaccagtag cgtgaataac
    18061 agcgccagaa tcgcgccgca aaaggccccg ccaaaacgca gcaccatctt ctggtacgac
    18121 gaaccgacat ttggattagc gacgatcacg caggtcagca tacaggtgtg aatgccttcc
    18181 cagtccacgc cgctgtaaaa ggtgtaacag atcaaacagg cgagcaatgt ttttaccgcg
    18241 tagcgtatat agtctggatt ggtaaaagca tcggcgacca ttgatggcgg tttggcggcg
    18301 ggcgttggcg gcgtattcgg gttcatctgg cccagttgta acaacgtctg gcagatattc
    18361 tccaggttac attcccgtgc cgccaccgct tcactttcac tgatccgcca gtcgctttgc
    18421 cagcactgac cttcagcaac ggcatgctgc agcttgttga tttctgaggc taatttttgc
    18481 cgaaattcaa taattgcctg agaatcagca aaagaggtgg gatcgtagcg attcagcgtc
    18541 gagtaaatgt aggttaccgt tgccacgcag ctttgccacc atgcgctttg agttcgccag
    18601 ttggcatcgt ccgcgagaca aaagacattc agtttttgca gcgccagcgc ctctctttca
    18661 atccgcgttt cgggtagcgg tgcgaggctg tctgtcaggt gactaatggc atcatcaagc
    18721 cgatcattaa gcgcctgatg catttgcgaa atggcacgac tgggaaacca cagcacgccg
    18781 attaacgtca tcagcaaggt tggatagagg ccaacaacga tacaccacag cgttaagcgc
    18841 acgaccactt ccggatagtc gagcatggcg gggaaggttt gcccgtaaat ggcgacaatg
    18901 gcgacagcga aaaagaccag tcccagccga tgggtgcgca tcaaaaacat gcagcccatc
    18961 aggatcggcc cggcgatgat caatcggatc aacggttcgc cgtatgacca tttgtagatc
    19021 aaaaacaggc tgccgatctc cagcaccgtg gcaaccacaa acaagatcgc cacaaatttg
    19081 gtgtaaaacg cgttcgactg aataccgtaa aacagcactg ccagcgataa cgccacaaaa
    19141 gggatctcaa aagtcatcga gatcagaatc accagcaggc agcctaccca gagttgcacg
    19201 atctgcggca cgcggccggg tcgccgctcg cttaactctt catgaaagaa cgccagcagc
    19261 ctgaccaccg gtaatggcag ggagttgagc gcgctcatta ttgcggctca agattagcga
    19321 ctgccgaagc gccgatgcgg aacatttccg ggtcaggttt atcgaccatg attttgaccg
    19381 gaaaacgctg ggcaacgcgg acccagttaa tagagcgaga aactttcggc aggccgccca
    19441 gcaccaggcc gccgtcattc ggtagcacac cgtagccaat cgaatccact ttaccctcga
    19501 aggttttgcc gctgtcactc atcaggcgaa tcgttgcggg tgtacctgag cgaatatttt
    19561 tcagatcggt ttcgcggaag ttggcgatca cgtaccagtg acgagtgtcg attagggtaa
    19621 aaatagggcg catggcagaa gcaaattgcc cgacggaggt tttgagggaa atgacccggc
    19681 catcaaacgg cgcgcgaacg gtcgccattt ccagatgcag tttggtcagg gcaatatccg
    19741 cttcgaccgc cgcacgctgg gcaactaata catccacgcc gctgacggcg ctggcggctg
    19801 actgcgcctg taacaatacg gcattaagat ccgcttctgc ggcgcgctgc gccgttcttg
    19861 cacggtcaac atcttccgct gagacaaaac cttctttcag taatggctcg gtgcggcgta
    19921 atgtatctgt ggcctgtttc gcggcggcac gtgctttttc taccgtggca ttaaccgagt
    19981 cggcaccaaa ctgttgggcg tcaacgctac gctgggtgag cataatttgc ttatccagcg
    20041 ccgcgaggga ggcttcagct ttcgccagat tggcttcgta cgggcgcggg tcaatgcgga
    20101 acagcaaatc gccctgtttg actgcctggt tgtcggtgac cgccagttct acaatgcggc
    20161 cgctgacttc cggcaccaca tcaatggtat ctgctgacgc gtaagcgtca ttagttgatg
    20221 gcgcactgtc tacgcgccag ataacgaaaa caagggcaac cagcgccaac gcaaccacta
    20281 acagagcagg gaatttactg cgaggagctt ttttcggcgt actttccatg agcgcgtctc
    20341 aaatagatta gaaaaatgcc agccagaaca gcatggcgta gacagcaaaa agggcggtat
    20401 aaataattcc gacaaatgcc agattgatat ttgcccgctg aataattcgc ctcgtgatta
    20461 gcgtcaaaat aaggctggca atggcgcaga aaaaccagct gggataataa gcgccgatca
    20521 ccgggattgc cggagataat gagcatcccg aaaccatgaa gacgggaatt atccaggctg
    20581 tttttttgag ttgcatcgcc atgcgagaga gaactgttgg catattaaat acatcctgtt
    20641 tgctgcgaac aatatctgta gtgaatatca atatccattg aaggcgtcag tatggctatt
    20701 ttcttgatga ataaaaatag ctttattcag ccaccgcatt cataacttat atattttatg
    20761 tgttaattga ttgtatgtat ttttataata aatgcttttg ggggcttaag cgcccccaaa
    20821 agtgagtggc attaatttgg ggttacgata ttcacccaca aatcgaaatt atccagacag
    20881 gcaataattt cttccagttt cgcgccattg ccaataattt tggctttctt cgcttttacc
    20941 agatccgcca ttttggcttg tcctgtcagc acggcgtgca gatcttcacg gctgatgtag
    21001 aaagaggcgt cggcttgcgg ttgcagtgtt ttacggtagt taagcacgct atcgttcagc
    21061 gtcaggttga ggttatcgcc gttgctcata ttgaagttca ggctgatatt tttacccgca
    21121 gctttcgcgc tatcgaggcg aacggacata aagtcgaaca gcatttcgac cgacatcccg
    21181 cgaatggtgt ccggggaacc ggtggtgccg tggctgaact tatgcacccc ttcgcgcagc
    21241 tctttcgcgc cggtcaggta gaaaccgcgc caggtggcgg actcggcctg atagcccagc
    21301 tgttcaaagt tattcgcttg cagattcttc gcgacctggt cacccgggtt ggcggcaatc
    21361 acctgtttca gcagttctgc cgaccagcgg taatcacctt gcttgttcgc ttcttgcgcc
    21421 aggctgatga cacgggcaga accgcccagc gcctgcacgt aacgtttacc catctccacc
    21481 tgaccatacg gatgcaggtt agccgggtta ccgtcgtaat agccaagata gaagttatac
    21541 accgcgcggg cgttgtggct gacagaaccg taatagccac ggctggccca gttattggca
    21601 agtgcaggcg gcagtttaat catgtcgccg atttcattca tggtgtagcc ctggttcgcc
    21661 aggtgcaggg tctggtcgtg aatgtacttg atagtatcgc gatatttacc aatataatca
    21721 ttgatatgct tattgccccg gaccggccag gtgtgcggca taaacaggac ttccgcgtcg
    21781 ttaccccaca tatccagcgt ttcgttcaga tactcggtcc acttgctggt gtcgcgggtt
    21841 ttcgcgccgc gcagagtgta gaagttgtgc agggtatgcg tggcgttctc ggcggtacac
    21901 agcgctttca gggccggaat atagaagtgc atttcggctg gtgcttcgct acctggggtc
    21961 atcaggaagt caaactccag gccgtcgata atcatcttct caccggtttt ggtgatggtt
    22021 ttgttcggtg cgatgatgct cgggtagccc gatgccagcg ttacgccaag accattgccg
    22081 atgttaccct gagggttatg cgccagcagc aggccgtagg agtaaagtgc acggtggctc
    22141 ataatattac ccgccagtac gttttcgctg atggcttcgt ccataaagcc agcaggggcg
    22201 ataacctgca ctttaccgga tttcacatca gcttcagaga taatgccttt cacgccgcca
    22261 tagtggtcgg tatggctgtg ggtataaata accgcaacaa tcggtttttg cgggcgattt
    22321 tggaagtaaa ggtcaagggc ggcttttgcg ctcggtggcg tcaccagcgg gtcgatgaca
    22381 ataatcccgg tatcaccttc gataaaggtg atgttggaga tatcctgacc gcgaacctgg
    22441 tacattctgt cggtgacttt aaacaggccg gaaataccgt ttaactgcga ctgacgccac
    22501 aggctagggt taacggtttc gggcgcgggc gcattgatat caaatttata atcttccccg
    22561 cgataatatg gtttgccatt agcatcgcgc agaatacctt catccagcag cggagcgata
    22621 aatccacgct gagcgtcgtc aaaatcctga cgatcgctaa acggtaatga tttggcgtaa
    22681 ttctgattaa cctgttgggt atattgagtg gcggctttcg cttcctcttt cgcgttagcg
    22741 gtatttacca tcatgccgct ggcggcagtt aacgcaataa caattctgct caaacggaat
    22801 aaccgagagt tattcatata aatctccatt tatcatttat gtcgtaaata tgtaatccat
    22861 acaaaatacg tcatttaaag ctggggttat cataattggg aatttgcgac tttaataagt
    22921 ggaagtgtga gctgaacgat ccattttatt aggcatttgt gctgaagaga gaaaattacg
    22981 cgaatttatt atctctgtaa ataatatgag gtgttttata tatttattat atatattgta
    23041 cttcggcaaa tactgagcac aatttatcaa tgcaaccggt gtttcttttt aaaaaacgta
    23101 ccggagcaat gggcggtcat gtcgctggca ccgcaggatt ctgcgttgct gtgccagcct
    23161 tttattcatg gtcaatgagt gaggtgattg ataaagacac caggtcgtca gagcgcactg
    23221 attatccgca ttatgccagt gaagttgcac tggcttttgg ttcacgagtg gttggcctcg
    23281 aactggcaaa aatgattgtg gatgcgtggc tgggcgcaca gtacgaaggc ggtcgtcatc
    23341 aacaacgcgt ggaggcgatt acggcaatag agcagcggag aaattgagat tcatccacta
    23401 cttgcatgga tgagtaatga ttaatgtgga tagagtttct ttttgaggtt ggctaatgaa
    23461 acgctttccg ctatttcttt tattcaccct gctcacgctg tccaccgttc cggcccaggc
    23521 cgatattatc gacgacacca ttggcaatat tcagcaggcg attaacgacg cttataaccc
    23581 tgaccgtggg cgagactatg aagattcgcg cgacgacggc tggcagcgtg aagtgagcga
    23641 cgatcggcgc agacaatatg acgaccgccg ccgccagttt gaagatcgcc gcaggcagct
    23701 ggacgatcgc cagcgccagc ttgatcagga gcgtcggcag ctagaagatg aagagcggag
    23761 aatggaagat gagtatgggc gatgaggttg ggtatggagc aggcatcgcc cctcacccca
    23821 atggggagag ggagaaaacc agcgcaatat tcaatatcga gcacaatcgg tcccctcgcc
    23881 cctctgggga gagggttagg gtgagggggc cagcgcgcac attccctctc atcacgccac
    23941 cccaatctcc atcccatcaa atcccgcctc aaaccccgac ggcagtgcgt tttccatcag
    24001 ccacgcatca aactggtggc tgatatgggt cagaatcacc cgtggcgagc ggataacctg
    24061 attcagcgcc agcacggtgt ttaaatcaca gtgactacgt ggcgcatcct cgcgcggcgg
    24121 gtggctgcaa tcaatcacca ttacctgcgg ctgattattg cgtaaaaatt tcagcgtttt
    24181 ttccggcaag cctgcggtgt cagacagcca cgccacccgg ctgtgtgccg tttccagcag
    24241 ataaccgaag gtcagttttg agtggttgag cggcaggggc gtgacctgta acccctgcaa
    24301 atcaaacacc acaaacggtt ccaccgtgtg gctgaaatca agcaggcccg gatgtttaaa
    24361 cagatcgtcg cagccctgtt catccggcgg gccgtaaacc gggatcggat cgcccacgcc
    24421 ccagcgcagc gggaatagcc cctgcacgtg atccatatgg tagtgcgtca gcaaaaactg
    24481 ctggaacgat ccgggcggcc agcgatcggc gagatcgtgc agcccggcgt cgatcagggt
    24541 gattgcgtcg ttaaacttca ctacgccgct gcatggctgg cggcgatact gcggtgagcg
    24601 ccgcgctctg gcgcaggcta cgcactcgca gccccatgcc ggaacgccct gtgcgccgcc
    24661 ggtgccggtg agcgtgaggg tcaggctcat gttacagcgc cttggtgaag cggaagtggc
    24721 tctgctcgta gccttcacgc aggtaaaaac ggtgcgcgtc gtggcgcttc acgttggtgg
    24781 aaagttcggt catttcggcc ccggcctggc gggcttcttc ttctgcccac gccagtaact
    24841 tactgccgac gttcagaccg cacgcctgcg gcatcaccac cagttcctga atttcgccga
    24901 tccagttgac atgatgcaga tgaaactgca aatgcaggcc gatcatgccg acaacttcgc
    24961 catccagcag cgccagatgg tagcgcatgc tcgggtagcg catgctcggg tcgcgcaggt
    25021 tggcgttaaa gcccacgcga aacgcgtggt ggtcaaactc cgcctgtttt agctcacaaa
    25081 tcagcgcgta aaccgcgtcg gtatcgtact gcgtggccgg gcgaagctca caagcaggca
    25141 tggtgtttct ccttctgatg aatcagcgtc agcagtgtgt cgaccgactg gcgcaggctg
    25201 ccgtcattgt tgagcgtatg gcaatcctgt ggagtatagc gggcggcgcg cgccagccgg
    25261 gcgttaattt cactggtgtt ttcacggcca cggtttttca ggcgctggcg caggatctcc
    25321 ggcgaaacct gtaaacagac gggcagcagc gccgattgat agcgcgcccg tgcctgcggc
    25381 aaatgcgcgc gtgaaccgtt gacgactacg tcgaatccag cgtgcagcca gaggtcaatc
    25441 tcgatgccga cgccataata cagaccgtta gcgtgccagc ttaaggccag caggttttgc
    25501 cccgcgcggg taaaaaactc ccgctcactc agggcgatat ggttttcact tccggcgctg
    25561 gcgtcgcgcg tgatgtagcg atgcgccacc agtaactgcg tttgttcccg caggcggagt
    25621 tccgccagca ggctgtcttt cccggagccg gacggcccca ttaaccaaat cagttttccc
    25681 atcatcagaa caccctttta ccctgacgcc agacgtggtc gatatgaatg tgattgccct
    25741 ggcgatgcgc cagcaccagg tcagcgcgtt taccctcgcc aatcaccccg cgatcctgaa
    25801 gattcagcgc ctgcgccgga tttttggtca ccagcctcac cgcctgcggt agcgtaaagc
    25861 ggttgctctc gtcatcggcg acgcggaacg ccgcatccag caggctggcg gggtagtagt
    25921 cggaagagag gatatccagc agaccaagct gcgccagttc actggccgcc acattgccgg
    25981 agtgcgagcc gccacgcacg atatttggcg cacccatcag cacgttcatg ccatgcttac
    26041 gcgaggcttc cgccgcttcg aacgtggtgg taaattcggc gatcacgctg ccaagttggt
    26101 gggattcggc aacgtgggcg tgcgtggcgt catcgtggct ggcgagcgca atttgtcgcg
    26161 cgcggcacag ggcggcgatg gattcgcgat tcggctgcga ccagcgtgcg gcgagcgcca
    26221 attgctcttc ttcgtactgc tgcatctgcg catcggtgag ggagtatttg ccctgataat
    26281 attcgcgata cttctcgcgg ttagcgaact ggcgctggcc cggtgagtgg tccattagcg
    26341 acaccagcgt caccggttcg cgctgaacca gtttttcaaa cagcggcagt gtggtgtgat
    26401 gcggcagctc gcagcgcagg tgcagacggt gctcggcgcg gttgacgccg cgtttctgcg
    26461 tctcttcgat ggtgttgatc atcttctcca gattctccag ctgatcgccg ccgtcgcgca
    26521 cgtcgccaat cgccaccgcg ttcagcacgg tggtgatgcc gcttgccacc atcagcgcgt
    26581 cgtggctgct catcgccgaa tgggcgggcc agtcgacttt cgggcgcggg gtgaagaatt
    26641 tatcgaggtt gtcggtatgc agctcaatca ggccgggtag tagccagcca ccttcgccgt
    26701 ccatcgcttc tggcaggcgg ctctggcttt cggcaaaggc gcggatttcg ccatcctgca
    26761 cctccagcga accgtgcacc acctcgtttt ccagcaccag cttaacgtta ttgataatca
    26821 tgaagaggct cccattgggt gcaggcggtc ggcgacgtca ttacgtacag cttcgtcatg
    26881 gaagatgccg acgatggctg cgccacgggc tttggcttcg tgaatcagtt ccaccaccgc
    26941 ggcgctgttt ttggcgtcca gcgaggcggt aggttcgtcg agcagcagaa tggggtagtc
    27001 gacgataaag ccgcgggcga tgttgacgcg ctgctgttcg ccgccggaga aggtcgatgg
    27061 tgccaggtgc cacaggcgtt ccggcacgtt caggcgggtg agaagacgcg cggctttagc
    27121 ggcgcaggct tcacgcggaa cgccggtatc gagcagcggc tgcatcacca cttccagtgc
    27181 tgagatacgc gggatgacgc gcagaaactg gctcacccag ccgactgtgg ttttgcggat
    27241 ttccaccact ttgcgcgctg gcgcggtgac caggtctacc cactcgtcac cgtgtttgat
    27301 ctggatttga ccttcgtcgg gtagatagtt ggcgtacagc gagcgcagca gggttgattt
    27361 gccgctgccg gagtgaccgt ggagcaccac gcattcgcct gcgttgacgg tgagcgaggc
    27421 acgttgtaag acgggcaggc gtacgccgtt ttgctgatgc aggatgaagg ttttactgac
    27481 gttttgtacg ttaatcattt ttcgcctcgt ggttcgggtg tcggatgcga cgctgacgcg
    27541 tcttatccga cctacgggga gcgcatttgt aggccggata aggcgtttac gccgcatccg
    27601 gcaccggctc aattctgcaa aaccgatgac accagcagtt gtgtatacgg atggtgcggg
    27661 tcgtcgagca cgcggtcggt taacccactc tccaccactt gcccctgctt catcaccagc
    27721 aaacggtccg ccagcaggcg ggcgacgcct aaatcgtggg tgacaatcac taccgcgagg
    27781 ttcagctcca ccaccaggcc gcgcagcagg tcgagcaggc gtgcctgtac cgacacatcc
    27841 agtccgccgg tcggctcgtc cataaacacc agcttcggat gcgtcaccag gttgcgggcg
    27901 atctgcaaac gttgctgcat gccgccggaa aaggtggtcg gcaggtcgtc gatccggttg
    27961 gcgggaattt ccacctcttc cagccacttc tgcgcggtgg cacgaatatc gccgtaatga
    28021 cgtgccccgg tcgccatcag ccgctcgccg atattgccgc ctgccgacac ctggcggcgc
    28081 aggccgtcga gcggatgctg atgcactacg ccccattcgg tgcgcagcag gcgacggcgg
    28141 tcggcctcgc tcattgcgta cagcgaacgg ctctcatagt gaatctcccc ctgctgcggc
    28201 gtcaggcgcg cggagattga tttcagcagc gtggtctttc cggagccgga ctccccgaca
    28261 atgcccagca cttcccccgg ccataaatca aaagagacat cgctaaagcc tttgcccggc
    28321 gcgtaaaggt gggtcaggtt attgaccgaa agtaacggtt gattcattgg ttttttgcct
    28381 cgctctgttg gcggcaataa tcggtatcgg agcagacaaa catgcggttt ccggcgtcat
    28441 ccagcaccac ttcatcaaga tagctgtggg tcgatccgca gatggcgcat ggctcatccc
    28501 actgctgaac ggtgaacggg tgatcgtcga aatcgagact ttccacgcgg gtaaacggcg
    28561 gcaccgcata gatgcgcttc tcgcgccccg cgccgaacag ttgcagggcg ggcatcatgt
    28621 ccatttttgg gttatcgaat ttcgggatcg gcgacgggtc catcacgtag cgcccgttca
    28681 ccttcaccgg ataggcgtag gtagtggcga tatgaccgaa gcgggcgata tcttcataca
    28741 gtttcacctg catcacgccg tactcttcca gcgcgtgcat ggtgcgggtt tccgtttcgc
    28801 gcggctcgat aaagcgcagc ggttcgggga tcggcacctg gaagataatg atctgatctt
    28861 cggtcagcgg cgtttcgggg atgcgatggc gcgtctggat aagcgtcgca tcgtccgtac
    28921 gttcagtggt gtttaccccg gtcacgcgct taaagaagtt gcgaatcgac acggcgttgg
    28981 tggtgtcgtc tgcgccctgg tcaatcacct tcagcacgtc gctttcgcca atcacgctgg
    29041 cggtgagctg aatgccgccg gttccccagc cgtagggcat cggcatctcg cgcccgccaa
    29101 acggcacctg ataaccgggg atcgccaccg cttttaagat ggcgcggcgg atcatgcgtt
    29161 tggtctgctc gtcgaggtag gcaaagttgt agccgctcag attagccatg gttctgctcc
    29221 tgttgcagac gtttgagtag ctccagttcg gcctggaaat cgacgtagtg ggggagtttg
    29281 aggtgcgaga caaagcctgc ggcttcgacg ttgtcggcat gtgccagcac gaactcttca
    29341 tcctgcgccg ggcctgtcgc gtgctcgccg tactccggag cctgcaacgc acggtcaacc
    29401 agcgccatcg ctttgcgctc gctcatgccg aacaccagcc cgtagccgcg cgtgaagtgc
    29461 ggcggctcat ccggcgggtc aataaaaccg ttgaccattt cacactcggt catcagcagt
    29521 tcgccgacgt tcaccgcaaa tcccagctct tccggcataa tcgacacgtc gatgtaacca
    29581 ctgcggattt cgcccgcgaa cgggtgattg cgtccgtaac cgcgctgggt agagtaggcc
    29641 agcgccagca aatagccttc gtcgccgcgc atcaactgct gcaaacggga agaacgtgaa
    29701 caggggtaaa ctggcggagt gcgggtgatg tcatccggct gtgcgccgct atcctcttca
    29761 aacttcgcca gcccctgacg cgccagcagg ctgaaaacgt gcggcgacgg ggattgttcg
    29821 ctgtctgcgg tggtcagcgt cggcgcttcg ccgtttgcca gcagggtgaa atcgagcagg
    29881 cgatgggtgt agtcgtaggt tgggccaagc aactgaccgc cgggaatatc tttataaacc
    29941 gctgaaatac gccgttcgag acgcatctcc gtggtgtcga gcggctcgct taccgccagc
    30001 ttcgccagcg tggtgcggta ggcgcgtagc aggaaaatcg cttcgacgtt atcgccgctg
    30061 gcctgtttca gcgccagcgc cgccagttcg cggtcggcaa tgccgccttc ggtcatcacg
    30121 cgatctactg caagattaag ctgctgttca atctgggcga cgctcagttc aggcaaatcg
    30181 gtattgcccc ggcgtcggct ctcttgcagg gcgtgggcgg cgtcgatctc cttctcgccc
    30241 cctttcacgg caacgtacat cagcacacct ccacatgagt ggttcgcggg atagccagca
    30301 gccgctcgcc gcaggtcagg atcaggtcga tgccgagcgg gaacgggtgc gggcgctcgg
    30361 tgagttcgtg cagaatgcac tccggcagct gcggagcgat cattcgttct tcggcaatac
    30421 ccgcgccagt aaggcgcagc atgcgcccgc cgctcaggct gacgacctgt aaaatcagcg
    30481 tcgcgcccgc ttccggcgca acagcggtgc cgctggaaag agcgttgagc tgttcgctgg
    30541 aaatcgcctc atccgttacc gcgaaggtcg ccagttccga ctgattgacc agcggcgcgt
    30601 tggtatgaaa acgcaggctc tggctgacga tatcgttact taatggcgcg gaaagccaca
    30661 ccggcgtgtc gttatcagcc agcgtcagca gcacgctggt ggtggcgata ttcagcggct
    30721 gccagccgcg tttgagctga tgcagggcga caatcacgcc cggctcgctc atggccttta
    30781 acaggcgacg aaaactgtgc tgggcatcct gcacaggaag cataaaagcg gtttccaggg
    30841 tcatgcgttg tctccgcgaa ccatcgtaaa gaagtcgacc cggctggcgt tcacttcggc
    30901 ctggcgtgcg gcaatgcgtg ccatacggtc agcgtccagt ggggcaataa gggtttctga
    30961 taagttttga aaatggcgag attgctgcat caacgcgtca atcagcgcgc agcgttcggc
    31021 gtgctgtttg tcgcgcccca gcacccagct gtagccgagg gggccgtcag tcaggcgtac
    31081 ggcggcgcgg gtcagcgtgg cgtcgccggc aaaaaagcgt tcgccggtac cgcccattcg
    31141 cggctgaatc tgtaccaggc cggtttcggc ggtgcggatc acttcatagt tggcggtgat
    31201 gttcagcgcg ttcaggcggg ctgccagttc agccggttgg ctgtgcgcca gcatggacat
    31261 ccagtgctgg cgggtcgcga tatctgcgtg cattcagtgc tccatagtga attcaatcat
    31321 gtcggcgcgc gtcaggctga cggagtactc cgccgggctg ctttcaccgt cacggtggtt
    31381 aagggtgcgc acgcacagca gcggcgacat attcgggatt tcaagacgct ggcactcttt
    31441 ggcctgggcg cggcgggcgc tgatccgcgt ctggctgcgg cgcagcgcaa tcccggtttg
    31501 ctcgcgcaga aaatcgtgca gcgagccgct gtcgaagcgt tgcagcgtcg gccaaagggt
    31561 gaggtccgca aagtagtggt cgattaaaca gagcgcgatg ccgttgaccc ggcgcagggt
    31621 gcgcaggtgg atgacgttct ctccctcggt tatccccagc gcgtcagcga cgtgaccaga
    31681 tgccgggcgc agcaccgaca gcagtttttc gctggtggga tggctgccct gatccagcag
    31741 gttctggcta aaacgcgcct gggcgttgag cgggtaatcg aacgggcgca tcagcaccag
    31801 cacgccgacg ccctgacggc gctgtaccca gcctttttcc accagttggt cgatggcgcg
    31861 gcgcagggtg tggcgattta cctcaaagcg cgccgccagc tgctgctcgg cgggaagata
    31921 gtcgccgcag cgatagtgtt gacgaagctc ctgctcaagt tttgcggcta tctcctgata
    31981 gcgtgttggg tagctggtcg gatgtgtaga caagtgcata gatatcaatg cctcgcttat
    32041 cagataaagt gcttacgcaa ccgttgagag aggaaatcca gcaggctgac cgtgacgatg
    32101 ataagcacca tcagggcgca ggtttgttgg aactgaaaac cgcgaatcgc ttcccacagg
    32161 gtgacgccga tcccgcctgc accgaccatg ccgacgacgg tcgccgagcg gacgttggat
    32221 tcgaagcgat agagggagta ggagatcagc agcggcatta cctgtggcag cacgccgtaa
    32281 aggatctctt ccagcttgtt ggcgccggtg gcgcgaatgc cttctaccgg gccgggttcg
    32341 atcgcttcca ccgcttcgga aagcagcttg gagagcacgc cggtggtgtg gataaatagc
    32401 gccagcacgc cagcgaacgg gccgagaccg acggcgacca cgaacagcat ggcgaagacc
    32461 atttcgttaa tggcgcggca ggcatccatc aggcggcgaa cgggctggta aacccaccac
    32521 ggcaccaggt tttcggcgct catcagaccg aaggggatgg agagaaccac tgccagcgcg
    32581 gtgccccaga cggcgatttg cagcgtgacg gccatttcgg tgaggtaatc ctgccactgg
    32641 ctgaaatcgg gcgggaagaa gtcggcaacg aaggttgcca tgttgccgcc gtctttaatc
    32701 agcgtaagcg gggccatttc cgcgccctgc cacgagacga ccagcacggc gagaacaacg
    32761 gcccagctca gaagcgagaa ccagctacgc ttgggtgggg cgatggtgat ggtttgcatg
    32821 tttggctccg tttaatgttt tctccctctc cctgtgggag agggccggga tgagggcatc
    32881 agcgtgcgca tttccccctc accctaaccc tctccctcaa gggagagggg accgatcgag
    32941 cacattactg caccgcttta ctcaccgaac tcatcgcgct taacgcgttg ttcaggcggt
    33001 ccaagtcatc cagctgcgcc tgaatctcgg tggttttcgc cagcttgtcc tgctcattca
    33061 gtcctttatt gctttttacg ccctgcatct ctttaaacag tgcgagctgg cgaatcggca
    33121 ccagttgcag gtcgctggag gcgcggaacg gggcccagcc caggcgttcc agcaccgctt
    33181 tctcttccgg cgtttttccg tagttcataa agaagtcgta gatcttgtct ttggtggttt
    33241 cggagagatt cttgcgccag acgatcggat cgcctgggat cagcggcgac ttccagatca
    33301 ccttcagttc tttcagcttc tctggcgcgg aggttttcag cttgtcgagg ttttcggtgt
    33361 tgttggtggc aacatccacc tgcttgttgg cgacggccag cgcgttggtt tcatgcccag
    33421 cgttgacggt gcgcttaaag tcgctggcgg agatattgtt tttggcaaag acgtagtaac
    33481 cggggacgag gaagccagag gtggagttag gatcgccatt gccgaaggtg agatctttcc
    33541 gcttcgccag cagatcgttc aggttgttga tcggactatc tttgttgacg atcaacacgc
    33601 tccagtaacc tggcgatcca tccgccgcga ccgtctgggc gaagacctgg ccgttggcgc
    33661 gatccaccgc ttccattgcc gacaggttgc cgtaccaggc gatatccact ttattgaagc
    33721 gcattccctg gataatgcct gcgtagtctg gggcaaagaa ggcgttcacc ttcacgccca
    33781 gcttcttctc catatcctgt aagaatggcg tccattgcgg tttcaggttt tgctgtgatt
    33841 ccgttgaaat aatgccgaaa ttcagcgcct tttcctgctc ttcggcgtgt gccgggctta
    33901 acagggtgct gaggctgaac atgctggtga aggccagcga ggcaattatc ttagcgttca
    33961 ttcgttttcc tcaatgatgg ggatgtcagg cagctttcgc gttctcttcg atgcgattaa
    34021 tgctgcggta gagatggtca aaacgttcgt tatcaaactg ttggctgctg ccgtcgtaga
    34081 aaacgtgccc ctggcgcagg gcgacgatgc gttcgcagta gcgcagggcg taatccacct
    34141 gatgcagcgt gacgactacg gtgatgccgt cgttctgatt gatgtcgcgc agggtgtcca
    34201 tcacgatgcg ggcggattcc gggtccagcg aggcgatggg ttcatcggcc agaatcacct
    34261 tcgcctgctg catcagcgcg cgggcaatcg ccacacgctg ctgctgtccg ccggagaggg
    34321 tggaaacgcg ttgatgggca aaatgcacca tgccaacgcg ggtcagcgcc tgtaacgcgc
    34381 gttgtttctg ctcgcgggtg aaccagctaa aacaggtgcg ccagaacggc gtgctgccga
    34441 gcgcgccaat cagcacgttc tccagtacgc tcaggcgatt caccaggttg aattgttgga
    34501 agatgtagcc ggtatgggcg cggcttttgc ggatatcgcg cgccagacgg ccttcgcgct
    34561 ggactgtgcg gcccagcagc tcgatatggc tgccggcgga tttatcgccg gtaatcaaac
    34621 cgcttaagtg acgtaaaagt gtggatttgc cggaacccga cggcccaagc agagccacca
    34681 tttcactgtg atgaatgttc agatcaaccg catgcagcgc ttgatgctga ttgaaggttt
    34741 tggcgagctt ctcgacacgg ataatcgttt gcatgatgca gcctccctaa aaaagtggct
    34801 ccatcgtggc ggattattgt gacgatttgg ttaattaaaa gtgactaaca gatgaagagt
    34861 taacgggaat tcgatgacag tgcgggaggg cgggcctccc ggcgggttat tgcgttggtt
    34921 gttgtttgac gacattaatc atccacggta cgccaaattt atcggtgact ttgccgaagc
    34981 catgcgccca gaaagtttcc tgccaggcca tttcaatttt tccgttagcg gcaaggttat
    35041 caaaccagcg ttttccttct tcgacctgtt gcgaatcgag caccagcgta aagccggagt
    35101 agctggcttt tcctgacggt atagcatcgc tcatcatgat gtcgcttccg gcaatgcgca
    35161 cgttggcgtg ggcgatggcg gtatcgggaa attgcattcc ggaagggcag ttctcggcgc
    35221 tgtcctgcgc tgattttggc atttcgccga agctgatttt atagagcagt tccgcgccca
    35281 acgtacgttg ataataggca atcgcgtcgg aacagttacc ggcaaaagag aggtagggac
    35341 ttaacggcat gatggtgacc tcagttaaga gaagccagtt aagtgtagtt cgaaatttat
    35401 acagatgaga gggaagcctg ataaggcgcg gtcagcgtcg catcaggcag tcggcatcgt
    35461 tgccggatgt ggcgcgagcg ccttatccgg cttaccgatc acaatacaat cagttctttt
    35521 tcacaaactc agatttcagt ttcatcggac caaaaccgtc gattttgcaa tcgatgttat
    35581 ggtcgccttc aaccaggcgg atgtttttca ctttggtgcc aattttcagc atcgaagagc
    35641 tacctttcac cttcagatct ttaatgatgg taacgctgtc gccgtcagcc agcagattgc
    35701 cgttagcatc tttaacgatc agctcgtcgc tttcctgtgc aggttctgtg tcgttccatt
    35761 cgtaggcaca ttccgggcag atgtacatgc cgttatcttc gtaagtgtat tcggagttgc
    35821 attttgggca gtgtggtaat gacatgtgga tttcctcaaa agtcagcagg gcaaaagcgc
    35881 caaaaaacgg cagattgccg aaaaaggccg caattataca caaaatccct gagtttgtcg
    35941 ggggtactgc tgattttaga cgacgatcgt tttcatttga aaggaaacgc aaaaagaaaa
    36001 taatcttttt gcctctattt attatttaag ataatgttaa tgctctacct gttctttttt
    36061 tattcacatg atatttttat ctttctgacg ttttaaacgc atcagaagac gttatttatt
    36121 ctgataaaaa acaacacact aaatataaaa tt
//
LOCUS       DAJVUJ010000021        35301 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.021, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000021 DAJVUJ010000000
VERSION     DAJVUJ010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35301
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.021"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..226
                     /locus_tag="OQQ48_000983"
     CDS             <1..226
                     /locus_tag="OQQ48_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCS1951831.1"
                     /translation="ARTASQAWSQRENDVASSLQSCLSRIKDISITLPQFPSLDKLLR
                     DLENQVCDAVVDRVNDHLPGNIDPWKDYNL"
     gene            238..933
                     /locus_tag="OQQ48_000984"
     CDS             238..933
                     /locus_tag="OQQ48_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotI/IcmL family type IV secretion protein"
                     /protein_id="HCS1951832.1"
                     /translation="MSEAQPTSPVSPEADNSGDIYNPVLRTIAQSEQRAALVPGLVRA
                     LLCSGAALCISLVANGIQYWHATGVTREYFATDNGRLVRLAPTSQPAWSQSDVMNFGS
                     MTLSEAFNLDFVHYRKQITTQAPRFSEAGFAGYVNALQASNILDTIKKEKFNLTTTIG
                     AGTLVNQGQMENGVWFWKFQYPVRMRLVGQTSTRPEQAFTFEITVRRVDPRIKPGGME
                     ISQMVSRNAAAGI"
     gene            944..1903
                     /locus_tag="OQQ48_000985"
     CDS             944..1903
                     /locus_tag="OQQ48_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraN"
                     /protein_id="HCS1951833.1"
                     /translation="MHIHHLIGALLIVCGTVTAADNPGWKNARSPQTGGKTAQATGNT
                     PPAAQLPGNLPPPGAALVQEGASLDSPLNAGEIRELRGIMADNERAISAPLTSVVPRI
                     SSLTVNLSPGASLPLVRTALHNVSVVTFTDVNGSPWPQSDPPYNAAPGHFDVQFNGNM
                     VSVTPLRAWAAGNVTVYLKGLSVPVILNITSGETDTTTLSQEIDSRLDLRIPRQGPAS
                     PVVSIPSEKIALHDATLQAFLDGVPPDDPAIRRLKFSGNVPDTTIWQHGDDLLVRSRA
                     MLRDEFEQTLASADGTRLWKLPVTPLLTFSVNGQSVHVTPELE"
     gene            1907..3238
                     /gene="traO"
                     /locus_tag="OQQ48_000986"
     CDS             1907..3238
                     /gene="traO"
                     /locus_tag="OQQ48_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraO"
                     /protein_id="HCS1951834.1"
                     /translation="MSEEQDTAAKSGKKLSALLVMGGVIIIGGGYLLLTWLSPENAAS
                     LSSVNINSTTSGGGRNVMETQHYRDLLRADNERGAAEALRNNETFIASLPRGLDTDTG
                     NDQKRDNPPDDQSGTSGQNASPPQEDQTAREKRREALQKLLARINTQHQDARPPVVAA
                     AMWSTTGTPGTAPHASPSGTVTSQHPRLQETSLATPATRQGIQLIPALTRVPAYIDTA
                     VDSDNAVSRVVASVPAGPWAGAVLYSQDARLAGQGMDIHFDRMMWRGMFLKVNAYALN
                     ERTGMSTVASDVNHRWFKHIVLPSVLGGVGNVGSLYKDANTQILQGNYGSITGRVGMP
                     DGEALAGVIAGGMAERGSQLLTRQAESEPYRQVTVSRGEVVSILFVEPVMSNDISTPS
                     SGTAPSESPSSGRPLTQAEAEHQASDRIRAAVLRKQNEMQQRYGIQQESYP"
     gene            3235..3948
                     /gene="traP"
                     /locus_tag="OQQ48_000987"
     CDS             3235..3948
                     /gene="traP"
                     /locus_tag="OQQ48_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraP"
                     /protein_id="HCS1951835.1"
                     /translation="MKNEHEDPGSFGETTAPASGKSFLKRQYMGYSLPVWAGCALIIL
                     VAIWYKFLRTPSEPPADAGFAVTPPAAESWAEPMTMTESGSGNNMPAPPVTPQADMQQ
                     LARDLKAELDRRDEEIRRTLTMLKDSITTLSNAIQKDEAWAQETRQLLEETRKQVHTL
                     QMQGALPAPTGNNRQPAKTLPSPLQGMKIMSVEKGMAWIRWQGSAWAVREGDSLGNIT
                     ITRIDPSSRAVITSAGTLR"
     gene            3948..4475
                     /gene="traQ"
                     /locus_tag="OQQ48_000988"
     CDS             3948..4475
                     /gene="traQ"
                     /locus_tag="OQQ48_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraQ"
                     /protein_id="HCS1951836.1"
                     /translation="MSIDALTAIENFASGIFSAGMEFLFTWGELLGIIGLIGHLMRAR
                     AEGRRSMGPGKFIAGIFICGMLVSLPSMINAGGTQMGFRADSFAPIAYVQPGSFGAAA
                     GAVNAILSLAKLAGVGFVMNGISIWRKSLLDGHTALSASESISKGNVKFVAGVLLVFN
                     DRVIDATLASLGIAF"
     gene            4522..4920
                     /locus_tag="OQQ48_000989"
     CDS             4522..4920
                     /locus_tag="OQQ48_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001605438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraR"
                     /protein_id="HCS1951837.1"
                     /translation="MSRLRAMAARLRAASVYVQLCLMACRDRLVRLLLLLPVMLLPHT
                     AMANTLFDMIKTVGDGARGSSSSFVAIGQVIGLVLFIGGLLGLKQIGKKPGAGLACLL
                     SILVGVCLAVVPELMGSGQKTLGLSPVSIS"
     gene            4977..5228
                     /locus_tag="OQQ48_000990"
     CDS             4977..5228
                     /locus_tag="OQQ48_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003665769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraS"
                     /protein_id="HCS1951838.1"
                     /translation="MSCELPPFTLLRTEHGNVIARDNRDNQEFVFAECYSTAIAALIL
                     AAVTLYTERKAYEKDPATHRPGPGTMAFPAPATGHGIQR"
     gene            5143..5961
                     /locus_tag="OQQ48_000991"
     CDS             5143..5961
                     /locus_tag="OQQ48_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraT"
                     /protein_id="HCS1951839.1"
                     /translation="MKKTPPLIAPVRERWLFPLPQPDTVFSGEALLPPPVLSTEGRCP
                     CCRRTVTHRFILEDSWPLQQMADTCRDTVVLLEKNLTRVMRLKKRPVPENADEKKKHT
                     RTLQDAERSLAQARLSARRLALRHVEKSQIVTTDALSENESDLLQPEGPPFHLCAFCH
                     AWHCLNGYAAAQGVMVWLPDLHPASVVALNARALQEIFSDNRQRVRQGRAVLNALVQN
                     RLAVEEKFRTWRPADFADALRRWPPAQRKTLREKMDGVALILLPDSFPDKKYVM"
     gene            6016..6375
                     /locus_tag="OQQ48_000992"
     CDS             6016..6375
                     /locus_tag="OQQ48_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1951840.1"
                     /translation="MDTLTAGYQFSADAFRRFIIRTLTDDIPPDGDEKEIARFILRHP
                     AFNGGWLLFLAAEIHERSRTHNSITVTVSVDNYLSWMDIAVSGLPELPDMPFCDFFIR
                     RQQFIYPGVPPTKSGEE"
     gene            6375..6755
                     /locus_tag="OQQ48_000993"
     CDS             6375..6755
                     /locus_tag="OQQ48_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1951841.1"
                     /translation="MKIHRISPETLITLIHAHLAGKADSTAKEEHRLLRRFLRDDDGR
                     LAGVLLNIAGILQFNRELSARHNYPATPLTEFSLRKRGKQLHLCLCSLRFFYIPPVFI
                     QNKRRKSIVVHLNKITYKQTHSIR"
     gene            complement(6728..7273)
                     /locus_tag="OQQ48_000994"
     CDS             complement(6728..7273)
                     /locus_tag="OQQ48_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001390888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1951842.1"
                     /translation="MKAENKILNLFHATTMSRGLQIIKDGKIRTDAPSVICWSLETTP
                     GWIYLSDQLDNAVHWGNKIACFSKDELFFYIFRVQVQEENCYPDTDDLQYVSWLSPEE
                     AENTDLYTCLSLCHSCRTDKELYTGCEISDYAVLPATTLYTDNSGHPQYDIVNTLKKH
                     IGKDEYNQYESLRQRIEWVCL"
     gene            7498..10542
                     /locus_tag="OQQ48_000995"
     CDS             7498..10542
                     /locus_tag="OQQ48_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCS1951843.1"
                     /translation="MNINRIIFAIEDCISTLSRYSVSAFFPDYCDLHTVIGLDQQDRE
                     RRPWLKAPYIATTKQGYYLSVFEISGALKEMDENPDQTAPGTLESLIAALAERMNTAW
                     KNSGHKISFVFERDPERGRDEIEAMLLPQRKSIARTGILLQDVLDEKVTTLTPWLVRE
                     RCWLTVWSSEELLSRTDRKDHQTRVRKLAEHAPPARFAQDPWRWTLSALKIRHDALLD
                     TLEQALTHDSDGLLIRLMDIHEVGREIRRQLERNSTPAVWQPHLPEDARPAGWRQGGD
                     TSVFHAPSLNLQLFTTQPETHGSLIQAGELWHGMVAITLPPQNLRTFNQLVRDVPRAI
                     PWRIRMDLMPDGMKALGVKKTLLTYSSFIPPLRPMYDSVMMLNDINKHDPVCIMTIVA
                     TTWGNSREVCTRNQALLKSALEGWGVCGTTTTFGDPRRAWVNTVLAASHSSGPIPLYP
                     PLSHALSLFPLNRAGSVWRGQGNLMMHTEDGSAWEVALASSQQNKHTELTPGAPGLGK
                     SVLINALSEIQIASAQKNLPFIAYIDKGFSAQGLVQLIRDSLPEQRKDEAVGIILSND
                     PDHTRNLFDVMYGARKPVTPEKNFMVSVLCALCVDTGTGQPCNPGDTRQIISSLVDLA
                     FREYGENNPRLYRAGTEPLVDLALEESGIAEQHDAGWWNAATWFEIRDMLHIAGNIPA
                     AQRAHYQAMPLLAEMSALLGQPSIRDVFGTVQRDNSEERLLDYIRRALDQGHSDYPMM
                     SGCTRFMLSPDTRVVAVDLNNVAGDKTPAGRLRTGIMYLLAGQIAGGDFVLPQYQEEI
                     RKNLDPRYHEVIFRRIEQLDQEVKTKVYDELHNAKGINFIWEALDTQELEQRKFGIRT
                     VLSTQLLQHYPPAVLKSANTLWLLRYRPDEIPFLRDNFGVPEVTLRRFLKMPEGAAPD
                     GSGVPVLAVFRVKNGTLARILKFTLGPLELWALNSSPKDSALRRALTQEVGSLRARQI
                     LAEHFPRGSATSLIEHRARTHDSENVIHELAAELIRKQGYNL"
     gene            10542..11162
                     /locus_tag="OQQ48_000996"
     CDS             10542..11162
                     /locus_tag="OQQ48_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraV"
                     /protein_id="HCS1951844.1"
                     /translation="MYSIHITPRCRRPARGQVFLKTLRVDGDSFSFTLPSACFTLRPS
                     PNPRVIFASDRRADGEMSGIRLALPGETGTLITTATWQVNVPERPPFDIQHVIHWLLP
                     APLTALMSDNTWLWPDVPGTPALTAGDWPVMESDTPTLKRVKRRTFTLIDNRNRQGWI
                     TRRFQCFPLAPLPESLSHRVPDILYPPKTEKKECKDNLQPHCLHSL"
     gene            11120..12325
                     /gene="traW"
                     /locus_tag="OQQ48_000997"
     CDS             11120..12325
                     /gene="traW"
                     /locus_tag="OQQ48_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraW"
                     /protein_id="HCS1951845.1"
                     /translation="MQRQSATTLLALLVGCAVIPATHAYSVNVVSSQPVTRQIMPELA
                     TIQGIMEEMVRMQTATGTAITQNSEKLATVIAQDGQATRQQMIFSNETHRLEEARKSF
                     SVPDSICSESASGIAAESRRAAASAAARLSQGGGVSSKPIRERLSRAADSPVREAYDS
                     AGIHAGYCTEAEYARFGGTDVCPAVGDLPGGDSQVRSLYQGAGTADTPAALTWDQKQI
                     DAATAYMKNTARPSAGRAPGKGEVGTQTGRTYVGLQNEYNGIIDAASHPQLSLIADST
                     PNEATRGALTEALQSPSAAAYFDRTASSEARTRGHMSQREFEAFEAGRRYANTDWQQD
                     LQGMEGDNLLRELLRTTALLNWQMNDLKEQIRQGNVIAGQQLALAARQYYGQRLGELS
                     QAMGQGSVR"
     gene            12322..12891
                     /gene="traX"
                     /locus_tag="OQQ48_000998"
     CDS             12322..12891
                     /gene="traX"
                     /locus_tag="OQQ48_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000650756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraX"
                     /protein_id="HCS1951846.1"
                     /translation="MKDDNSIDEKKPAPRLRRIGDIGINLFAPLAETRWMLRSCRHML
                     DQHKTRLKRLMPDRQPESPVCMTWEEAVRASGLTVDELDRRLRRRRMFWRTGFWLMMI
                     PVLLLCGMALAATALPVITLIRILVCLLLMSTIAALCASKALIASYRLWQLNERRVSE
                     DEQGTFPDYLQARHGWKQATWLAITHRQY"
     gene            12966..15131
                     /locus_tag="OQQ48_000999"
     CDS             12966..15131
                     /locus_tag="OQQ48_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotA/TraY family protein"
                     /protein_id="HCS1951847.1"
                     /translation="MKIIPRALSAGLALAAVPAQAFVTYQDIVSAATNPDDLSRQALV
                     TIFGDVVTNPLTASSNTIIGNLFMVFNAIIATMGIVWFLFIGIRHAVRTGHQGSVFNN
                     GRDVVGILSVVSGFLMIVPTASGWSLAQLVMLWGASIMGIGSANIMVQTAAENIAEGY
                     SMTVQPVHVSTRTAARGILEMELCKHAMNTGLDDFNRTARSSTPPMSESSRTENGRYT
                     VTVSNGSATCGSVSLSVKGNGSNKQSALAHFFNPFIETEYQGVLTAQRSAMDTMLRDL
                     DSAAQEFVSAYRTRRDTGNGNLPDIESRIQKAANRYEETVQKALPADNAETERREQLK
                     SYLTTYGWVALGAWYQTFATANQRLEDLANRAPTVSGVSSLGEAGDTEFLKEVKGAYQ
                     AQLQNSAYTPALGTATSASEYHASKTQDPQSAILKLIGFNLTRMTNWTATYLSGTGTT
                     TNQLNPLIKMKNIGDYTMVTTETIWAGYTTVRVLSTAGSNSVFGRLVNAVSGLSDAAK
                     AFLEASAPPLYFLLFLLFCAGFSLSIYLPFIPFIFWMTGIGNWIVSVLIGCAAGPLWA
                     ATHLGTSQDRGSRAAYGYIYLIDSMIRPLLMVFGFFFASVAIVAGGTILNALYAAALM
                     NVQINSVTGVFSMAGFLMIYARACTTLVAAVFALQAYLPDHVINFLGGRDGVNMLGNM
                     VSSVKDIIAGGNTNMRRTPGVREEKLKNINRDDKDGIKQ"
     gene            15219..15872
                     /gene="excA"
                     /locus_tag="OQQ48_001000"
     CDS             15219..15872
                     /gene="excA"
                     /locus_tag="OQQ48_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid IncI1-type surface exclusion protein
                     ExcA"
                     /protein_id="HCS1951848.1"
                     /translation="MNTTKSRYPSWWAYYYLIRTAYFIFGVPFLLLCSLGFTGMMLTE
                     PYIETDIQAYLYTYGSWLLLILPSLWLYMKTRAKKKKVQQVVQQIKESSPFAPTSDYE
                     QLSFSKSCYFGIDIKNGTMLYVRIYPNNVMDVIGLDIHNFTRTVAEDGKLEIHTTYVS
                     LPMIPLEISGISSRTLANTMHTMAARGYEYKERFPQMIRYRVKEWEKVAGVPVAEVF"
     gene            16146..17732
                     /locus_tag="OQQ48_001001"
     CDS             16146..17732
                     /locus_tag="OQQ48_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001506542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnsA endonuclease N-terminal domain-containing
                     protein"
                     /protein_id="HCS1951849.1"
                     /translation="MSESRSNFPARHGTPWNTEELQRLLVSIAEGVSLKKLTQTHQRS
                     SGGIRGAIKRLLPSEFLEFNDINTVNDLSNYFNKIPRQEHNSIINNLISLSTAKTITH
                     DKVEIQKNSFKQNTPLPLCQPNTDFKDYIADEHDVIMLIHSAIESLSNKRHQYILLMR
                     LGTDDSPHSLADIAVKLGISRERVRQLQERALGLLAAKSRYEGTPGACLKALINSIGN
                     SPYDIAVWIYRIVHRDFVTSSELAAKFIIFAAGIPKARRDEIKNSLSLISQLQKQKLR
                     ERRAELAHAHAQERARIKKERVEFIFSKWLDNTHWPKYIAPPPPVEQLCSQRAVNDTD
                     ISGFFYSQKLGRTVQYESGLEFKIMNLLECCDQVLFYQEQPFSIPYCFKNKSKKYYPD
                     LLIATVDGRCLLMEVKPTDRMTLSLTRIKSNAGRKWAHTRGWGWLVISDRFSLRQLEE
                     HAISTNTWKLIEAELKTSRILAWREMMELRTRYEIHRLDLIAYIIQSGAEVDNFYRIT
                     PKISNNTSNQRQPNESDCHN"
     gene            complement(17870..18022)
                     /locus_tag="OQQ48_001002"
     CDS             complement(17870..18022)
                     /locus_tag="OQQ48_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HCS1951850.1"
                     /translation="MPQRTFLMMLIVVCVTILCFVWMVRDSLCGFRIEQGNTVFVATL
                     AYEVKR"
     gene            18352..18594
                     /locus_tag="OQQ48_001003"
     CDS             18352..18594
                     /locus_tag="OQQ48_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1951851.1"
                     /translation="MKDEIHSTSINPDVVEQFLALMNTHCKSSEEALTMLSTLCYNLM
                     MTCGIHSMDSQMLNGHGISMQLQGIDIAEKPGKNIH"
     gene            18885..19253
                     /locus_tag="OQQ48_001004"
     CDS             18885..19253
                     /locus_tag="OQQ48_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001456887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1951852.1"
                     /translation="MKDNNTTATPETWKMRFLANGTSGTWFYTDDIAVTDAWLRAHPD
                     DVEVIPLCRIQPRQHSHEQHIEELRAALARMLHMHHMMLETTHYRENITDARCIREMH
                     EAPLQALNILSRYPHKKDEV"
     gene            19257..19472
                     /locus_tag="OQQ48_001005"
     CDS             19257..19472
                     /locus_tag="OQQ48_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1951853.1"
                     /translation="MWLLIFWLSSPFNVSSGDARQPTLAVHSQAFHTERACRDALRAI
                     KKENVGEFSLRGVCVSDGNTKEEAESS"
     gene            complement(19670..20293)
                     /locus_tag="OQQ48_001006"
     CDS             complement(19670..20293)
                     /locus_tag="OQQ48_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001466373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCS1951854.1"
                     /translation="MQEHIDYQIEKYQFRARNESPRLMRQWEDVMRECHDTGAGARER
                     LRIALLNVDYVTSFELPFRLLLTRTPQMIDSVRREWQLSQKKVVFNERQRGCVYSLQN
                     DLSGVPDSFSYSLATRIRRADSYGMTTRCFTDIAGQVKAPTARLKLALESGLIVTALD
                     GLFWLGIQRIAADVQRLRQSGMTIITRDVEVSDSLTGTTRVVSGYSL"
     gene            20451..20918
                     /locus_tag="OQQ48_001007"
     CDS             20451..20918
                     /locus_tag="OQQ48_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCS1951855.1"
                     /translation="MIKYIFSLTIIFSASLCAAQIQGHVIRVLDGDTLEILQDKKPVR
                     VRLANIDAPEKKQPFGRWSATQLKDLVAGKPVTVQYAQTDRYGRILGRVYTADGTEAN
                     RWQVRNGAAWVYPDYNNDYTLPPLQRDARALKRGLWADKNPVPPWTWRKQNKK"
     gene            21068..21244
                     /locus_tag="OQQ48_001008"
     CDS             21068..21244
                     /locus_tag="OQQ48_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1951856.1"
                     /translation="MNLVDAFVTKVISGPYEEYGKWWVDVEYISWGVPGKSRLMFDSR
                     EQAQEVQEGFRFLT"
     gene            complement(21304..21558)
                     /locus_tag="OQQ48_001009"
     CDS             complement(21304..21558)
                     /locus_tag="OQQ48_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1951857.1"
                     /translation="MNRLQVLAKTSGGAGFGALAGEELSITDTIENVATGNVRANHGV
                     LSPLMEKRMINLLIIVLRAVVAVANAVIALLELIRELVDL"
     gene            22199..23497
                     /gene="trbA"
                     /locus_tag="OQQ48_001010"
     CDS             22199..23497
                     /gene="trbA"
                     /locus_tag="OQQ48_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TrbA"
                     /protein_id="HCS1951858.1"
                     /translation="MSYQHSPTTEDPMNIWYIVGTICLLFALLIWLFLPDIVFATCLI
                     LHTMWGMTDWGPFHHFAAPRYNLLANTANNAATITFSQWLDVMSQTVGILWLILLPMT
                     IGFLWMWFQHPAQPRFTRRPLNIHTLPHLFSSLSPAIAPVLADGDSNRLFHGQKRPER
                     RVALTPEAFVEQNNLIRNMQLDVAATRQCFMAQLGQPLTTWKDMAPHEKALFAVFGLQ
                     FFLGDRKSAVSLMNNLNLSCRLKSKRDQGRFSTPVYSLARNAFIRVIKTEDAQQWLRQ
                     HRYVRSGLVWLYAHDLRLTPPNWLWLKGVDRTLFYALHRANTTKGFIEGAGVVAVARA
                     ENEASRLGLPCPEPCVEEAIEGLRRDMLGLGLIWDEPQPDRDRKRQIRTRWSLTDDVI
                     PRRHDNDEDTDTGETTETTETTETTETTEKQHPADKEKEQ"
     gene            23494..24618
                     /locus_tag="OQQ48_001011"
     CDS             23494..24618
                     /locus_tag="OQQ48_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HCS1951859.1"
                     /translation="MTIHYTATLNNKHLSLTALYPDGNREVIAILPVTELGALINRNN
                     RLMHLSFSSPFREITETVLVDTVDAERPGHHIGRMLSAIDACLEAEYAGKGKHPSRKN
                     GFWRTLMCGFIAGIALPATVAGYHWMKAEPPSTLAYPTSQEWRAEPVTDTETPRPHTE
                     HHVNSIPARPAPTQQEPSSAPAPTAVTEEQHVQAAEARRNLATVLKRNADRGMFTINL
                     STGHERTLYAFLDPACINCRLLEPALKQLAREFSVVIYPVSVIGGESSTKRTAPLLCE
                     PDSQKRATGWHQLYSADSGMAPTGDAPAAQDGQCLTAARAAIDVNNQAFRQFGFGGTP
                     WVLSDTGWHLPSGILKDATSVRLFLKATDSEEYHNEPAPR"
     gene            24599..26902
                     /locus_tag="OQQ48_001012"
     CDS             24599..26902
                     /locus_tag="OQQ48_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HCS1951860.1"
                     /translation="MSQHHVNPDLIHRTAWGNPLWNALHNLNITGLCLAGSIITALIW
                     PLALPVCLLFTLVTSVIFMLQRWRCPLRMPMTLSLDDPSQDRKVRRSLFSFWPTLFQY
                     EADETFPARGIFYVGYRRINDIGRELWLSMDDLTRHVMFFATTGGGKTETTFAWLLNP
                     LCWGRGFTFVDGKAQNDTTRTIWYLSRRFGREDDIEVINFMNGGKSRSEIIQSGEKSR
                     PQSNTWNPFAFSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVRERK
                     TMSLQMLREHMTLEGMAKLYCRGLDDQWPEEAITPLRNYLQDVPGFDMSLVRTPSAWT
                     EEPRKQHAYLSGQFSETFTTFAETFGDVFAADAGDIDIRDSIHSDRILIVMIPALDTS
                     AHTTSALGRMFITQQSMILARDLGYRLEGTDAQTLEVKKYKGRFPYICILDEVGAYYT
                     DRIAVEATQVRSLEFSLIMTAQDQERIEGQTSATNTATLMQNAGTKFAGRIVSDDKTA
                     RTVKNAAGEEARARMGSLQRHDGVMGESWVDGNQITIQMESKINVQDLIRLNAGEFFT
                     VFQGDVVPSASVYIPDSEKSCDSDPVVINRYISVEAPRLERLRRLVPRTVQRRLPTPE
                     HVSSIIGVLTEKTSRKRRKKHTEPYRIIDTFQDQLKRTQTSLDLLPQYDTDIESRANE
                     LWKKAVHTINNTTREERRVCYITLNRPEEEHSGPEDIPSVKAILNTLLPLEMLLPVPN
                     LTASPPHKKNVAQTPSGGKQESRKKRF"
     gene            27369..27599
                     /locus_tag="OQQ48_001013"
     CDS             27369..27599
                     /locus_tag="OQQ48_001013"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1951861.1"
                     /translation="MKTNMGSTKNGARVPHVRNRFTSMDIGMNSQILPDMLINFALIN
                     ITDRKNEGTNTIDGNWQADERRRYRDNVRIYF"
     gene            complement(27697..28128)
                     /locus_tag="OQQ48_001014"
     CDS             complement(27697..28128)
                     /locus_tag="OQQ48_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tolA family protein"
                     /protein_id="HCS1951862.1"
                     /translation="MVLRSIWIEEQYMGVKKIVMLAILVSFVAGCAPLHPSNCHKTTA
                     LGSCSSGRWDDQDEWGEQARGIRDAINAKLDDPQKWKRKKCRLHMEFSRDGTALKIST
                     SNGDKAYCEAIKSAAHKAKFPAFNNPEVYRDFQKSGFDMRG"
     gene            complement(28196..30907)
                     /locus_tag="OQQ48_001015"
     CDS             complement(28196..30907)
                     /locus_tag="OQQ48_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HCS1951863.1"
                     /translation="MNAIIPHKRRDGRSSFEDLIAYTSVRDDVQENELTEDSEIKSDV
                     PHRNRFRRLVDYATRLRNEKFVSLIDVMKDGSQWVNFYGVTCFHNCNSLETAAEEMQY
                     KADKAVFSRNDTDPVFHYILSWPAHESPRPEQLFDCVRHTLKSLELSKHQYVAAVHTD
                     TDNLHVHVAVNRVHPETGYINWLSWSQEKLSRACRELELKHGFAPDNGCFVHAPGNRI
                     VRKTALVRERRNAWRRGKKQTFREYIAQMSIAGLREEPAQDWLSLHKRLASDGLYITM
                     QEGELVVKDGWDRAREGVALSSFGPSWTAEKLGRKLGEYQPVPTDIFSQVGTPGRYDP
                     EAINVDIRPEKVAETESLKQYACRHFAERLPEMARNGELESCLDVHRTLAKAGLWMGI
                     QHGHLVLHDGFDKQQTPVRADSVWPLMTLDYMQDLDGGWQPVPKDIFTQVIPGERFRG
                     RNLGTQAVSDYEWYRIRMGTGPQGAIKRELFSDKESLWGYTTVQCESLIEDMIAGGNF
                     SWQACHEMFARKGLMLQKQHHGLVIVDAFNHELTPVKASSIHPDLTLSRAEPQAGPFE
                     IAGADIFERVKPECRYNPELAASDEVELGFRRDPVLRRERREARAAAREDLRARYLAW
                     KEHWRKPDLRYGERLREIHAACRRRKAYIRVQFRDPQLRKLHYHIAEVQRMQALIRLK
                     ESVKEERLSLIAEGKWYPLSYRQWVEQQAVQGDRAALSQLRGWDYRDRRKDKRRTTNA
                     DRCVILCEPGGTPLYEDTGVLEARLQKDGSVRFRDRRNGELVCVDYGDRVVFYHHQDR
                     NELVDKLNLIAPVLFDREPRMGFEPEGSYQQFNDVFAEMVAWHNAAGITGSGHFVISR
                     PDVDLHRQRSEQYYHEYIRQQKSISGGHGASYAPVQDNEWTPPSPGM"
     gene            complement(30919..31251)
                     /locus_tag="OQQ48_001016"
     CDS             complement(30919..31251)
                     /locus_tag="OQQ48_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nikA protein"
                     /protein_id="HCS1951864.1"
                     /translation="MSEKKTRTGSENRKRIVKFTARFTEDEAEIVREKAESSGQTVST
                     FIRSSSLDKVVNCRTDDRMIDEIMRLGRLQKHLFVEGKRTGDKEYAEVLVAITEYVNA
                     LRRDLMGR"
     gene            31484..31819
                     /locus_tag="OQQ48_001017"
     CDS             31484..31819
                     /locus_tag="OQQ48_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HCS1951865.1"
                     /translation="MASKKFYSDDDIQLAKAALSELPDLTAQRKTLRDFLDAIRDDII
                     VLVRTKGYTLADVRNTLKNAGYEVGEKALRDIIREAESKKPSRRSSSKTAPKKTDSGR
                     KDGIDMNNN"
     gene            complement(31905..32753)
                     /locus_tag="OQQ48_001018"
     CDS             complement(31905..32753)
                     /locus_tag="OQQ48_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HCS1951866.1"
                     /translation="MNQALPTADLNTAGTTDVIPSVAIDRIIAQRNEGIALFMQAMEC
                     LATARRILLDASGDIFLYGFEDCVTDSVRCMDKPEEAKRNIIRLADRKIWDRLMTDTG
                     MYTFMSSCQRDEWNSQLMSDTCPEITLDNVLATFRHLNASKMQTFEQGLIDVYRKLSW
                     DYRTNNPCRLGKKIIIENLLYRWSNGRVTLDCSGREALDDLVRPFYLLEGRNVPDFRN
                     SIGAQYGEFLGNGDNVGKLLEGEYFTVRGYQKGTVHIVFKRSDLVEKLNDIIARHYPG
                     VLPPRV"
     gene            33332..33583
                     /locus_tag="OQQ48_001019"
     CDS             33332..33583
                     /locus_tag="OQQ48_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003980153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HCS1951867.1"
                     /translation="MTGWELRIWRKSMLWSREKASREFGITQRTWHAWENAEQVDVTV
                     WRTTQALSVRDLLPHMQGMRKADIIRRLENELGETAEDV"
     gene            complement(33614..33883)
                     /locus_tag="OQQ48_001020"
     CDS             complement(33614..33883)
                     /locus_tag="OQQ48_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014358130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1951868.1"
                     /translation="MRTTKPGKIPGFFVSRSRANSMTIAERLIQKGFDEGFKEGFKQG
                     FKQGVLEVAREIACRLRDIGWPPERIQEVTGLSGEELKKLFPDEQ"
     gene            complement(33883..34821)
                     /locus_tag="OQQ48_001021"
     CDS             complement(33883..34821)
                     /locus_tag="OQQ48_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HCS1951869.1"
                     /translation="MSKKKNTTTPTPHDAAFRSFLANPDVARDFLELHLPAEYRQLCD
                     LSTLKLEPATFVEPDLHQYASDILWSVKTTGGEDGYVYTLIEHQSTENLYMPFRMLRY
                     SVAAMQRHLEQHKTLPLVIPVLFYHGERSPYPYSMNWLDCFENPALAAKIYTKPFPLV
                     DITVVDDNEIMNHRRMAALTLLMKHIRHRDMMELLDKLPQVMVEISDEQVRVLIHYIV
                     NAGDSVSPEFMRALAERLPQHEDKLMTIAERLEQKGRQEGRMEGRMEGRMEGRMEGAL
                     EKALAIACQLQKMGMTPEQIKQATGLSDDELKKITH"
     gene            complement(34818..35207)
                     /locus_tag="OQQ48_001022"
     CDS             complement(34818..35207)
                     /locus_tag="OQQ48_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CbiX"
                     /protein_id="HCS1951870.1"
                     /translation="MKSCFDTDKAFHMSFDEVTFNADRIRSKLELLSEELMPRCGLVY
                     ELYQRRLSAAIDNFVATLPTEQHLLVLELAREEFDYLSAEEIAEEIRRDSERGYCCHG
                     FERNCCPLGCGDLGDDCCDAADLMEEP"
BASE COUNT         8845 a         9845 c         8609 g         8002 t
ORIGIN      
        1 ggcgcgcaca gccagccagg catggtcaca gcgggagaat gacgtcgcct cctccctgca
       61 gtcctgcctg tcccgcatca aggacatcag tatcactctt ccgcagttcc ccagcctaga
      121 caagctcctc cgcgacctgg aaaaccaggt ctgtgatgcc gtcgtggatc gggtgaatga
      181 ccatcttccg gggaatatcg acccctggaa agactacaac ctctgaacag gagcaccatg
      241 agcgaagcac aacccacctc ccctgtctca ccggaagcgg ataacagcgg agatatttat
      301 aaccccgtgc tcagaaccat cgcacagtca gaacaacggg ccgcgctggt acccggtctg
      361 gtcagggcgt tgctgtgcag cggcgcagca ctctgcatca gcctggtggc taatggcata
      421 cagtactggc atgccaccgg cgtgacgcgc gaatactttg ccactgacaa tggccgactg
      481 gtccgtctgg caccaaccag ccagccggcc tggagtcaga gcgacgtcat gaactttggc
      541 agtatgacgc tgagcgaagc cttcaatctc gattttgtcc actaccgaaa acaaatcacg
      601 acacaggccc cccgcttttc cgaagccggc tttgccggct atgtgaatgc tcttcaggcc
      661 tcgaacattc tcgacactat taaaaaggag aaatttaacc tcacaaccac tatcggggca
      721 ggtaccctgg tcaaccaggg acagatggag aacggcgtct ggttctggaa attccagtat
      781 ccggtcagga tgcgcctggt cggtcagacc agcacccgcc ccgaacaggc cttcaccttt
      841 gaaatcactg tccggcgcgt tgacccccgc atcaaacccg gcggcatgga aatcagtcag
      901 atggtttccc gtaacgccgc agcaggaatc tgagactcac atcatgcaca tacatcatct
      961 tattggcgca ctgcttattg tgtgcggcac tgtcacagct gcagataacc cgggctggaa
     1021 aaatgcacga agcccacaga ccggcggaaa gaccgcacag gcaaccggga acaccccgcc
     1081 agccgcacaa ctcccgggaa acctgccgcc tcccggcgct gctcttgtac aggaaggcgc
     1141 atcgctggat tctcccctga atgccggtga aattcgtgaa ctccgtggga taatggccga
     1201 taacgaacgg gccatcagtg ccccgctcac cagtgtggtg ccccgtatca gttccctcac
     1261 ggtgaatctg tctccggggg ccagcctgcc ccttgtccgt accgctctgc ataacgtcag
     1321 cgtggtgacc ttcacggacg tcaacggcag tccgtggcca cagtcggatc cgccctataa
     1381 cgctgcaccc ggtcactttg acgtgcagtt caacggcaat atggtgtccg tcacccccct
     1441 gcgcgcctgg gctgccggga atgtgacggt ctatctcaaa ggattgtctg taccggtcat
     1501 cctgaatatc accagtggtg aaacggacac cactaccctg agtcaggaaa tcgacagtcg
     1561 tctggatctg cgcattcccc ggcagggacc tgccagcccg gtggtgtcca taccgtcaga
     1621 aaaaattgcc ctgcatgacg ccactctgca ggcctttctg gacggtgtgc caccggatga
     1681 tcccgccatc cgccgcctga aattctccgg taacgtcccg gacaccacca tctggcaaca
     1741 cggcgatgat cttctcgtgc gctcacgggc catgctgcgg gatgaatttg aacagacgct
     1801 ggcatcggct gatggtacgc ggctgtggaa actgccggtg acccccttac tcaccttctc
     1861 cgtgaatggt cagtccgttc acgtcacccc ggaactggag taaatcatga gcgaagaaca
     1921 ggacacagcc gcaaaatccg gcaaaaagct gtcagccctg ctggttatgg ggggcgtgat
     1981 tattatcggg ggcggatacc tgctgctcac ctggctgtcc cccgaaaacg ctgcatcgct
     2041 ctcgtcagtc aatatcaaca gcacaacgtc cggcggcgga cggaacgtga tggagacaca
     2101 gcactaccgg gatttactgc gggctgacaa tgaacgtggt gctgcagaag cgttacgtaa
     2161 taacgagacg tttatcgcca gtctgccccg gggactggat accgataccg gaaacgatca
     2221 gaaaagggat aatccccccg atgatcagtc aggcacttcc gggcaaaacg catcccctcc
     2281 tcaggaagat cagacggccc gggaaaaacg gcgtgaggca ttacagaaac tgcttgcccg
     2341 tatcaacaca cagcatcagg atgcccggcc accggtagtg gcagcagcca tgtggagcac
     2401 caccggcact cccgggacag cccctcatgc gtcaccgtcc ggtaccgtta catcacagca
     2461 tccccgtctg caggagacga gtctggcaac accggctaca cgtcagggga tccagctgat
     2521 accggcactg acccgcgtac cggcttacat cgacaccgca gtggactctg acaatgcggt
     2581 ctcccgcgtg gtggcctccg tacctgccgg gccatgggcc ggagccgttc tctacagcca
     2641 ggacgccaga ctcgcaggtc agggaatgga tatccacttt gaccggatga tgtggcgggg
     2701 catgtttctg aaggtcaatg catatgccct gaatgaacga acaggaatgt caaccgtggc
     2761 cagcgacgtg aaccaccgct ggtttaagca catcgttctg ccgtccgtac ttggcggggt
     2821 gggcaatgtc ggaagtcttt acaaagacgc caacacacag atccttcagg gaaactacgg
     2881 cagtattacc ggcagggtgg gaatgcctga tggagaggcg ctggccggcg tcattgcagg
     2941 tggcatggcc gaacggggca gccagctgct gacccgccag gcagagagcg aaccttaccg
     3001 tcaggtgaca gtatcacggg gcgaggtcgt ctccattctg tttgttgagc cggtcatgag
     3061 caacgatatc agtacgccgt cctccggcac tgccccttct gagtccccct cctccggacg
     3121 tccgctcacc caggcggagg cagagcacca ggcctctgac aggatccggg cggcggtcct
     3181 ccgtaaacag aatgaaatgc agcaacgcta cggcatacaa caggaaagtt atccatgaaa
     3241 aacgaacatg aagatcccgg cagctttggc gaaacgacgg caccggcatc cggcaaatcc
     3301 ttcctgaaac gccagtatat gggatacagc ctgccggtct gggccggctg tgccctcatc
     3361 atcctggtcg ccatctggta caagtttctg cggacaccct cagagccccc tgcagatgca
     3421 ggctttgctg tcacaccgcc cgctgctgag agctgggcag agcccatgac catgacggag
     3481 tccgggagcg gaaacaacat gcccgcaccg ccggttaccc cacaggcaga catgcagcaa
     3541 cttgcccgtg atctgaaagc agagctggac agacgggatg aggaaatccg gcgcaccctg
     3601 accatgctga aggacagtat caccaccctc agtaacgcca ttcagaagga tgaggcctgg
     3661 gcacaggaaa cccgtcagtt gcttgaggag acccgtaagc aggtgcatac cctacagatg
     3721 cagggagcac tgccagcccc gaccgggaat aacagacagc ctgcgaaaac cctgccttca
     3781 cctctgcagg gcatgaaaat catgtcggtg gagaaaggca tggcctggat ccgctggcag
     3841 ggcagtgcct gggcagtacg ggaaggcgac agtctgggaa acatcaccat cacccggatt
     3901 gacccgtcca gccgggcagt catcacctct gccggtacat tacgatgatg agcattgatg
     3961 ccctgacagc catagagaac tttgccagcg gtattttcag tgcaggcatg gagtttctct
     4021 ttacctgggg agagctgctg gggattatcg gccttatcgg tcatctgatg cgcgcccggg
     4081 cagaaggacg tcgcagtatg ggaccgggaa aatttattgc cggtatcttc atctgcggca
     4141 tgctggtcag tctgccctca atgatcaatg ccggtggtac ccagatgggc ttcagggcag
     4201 attctttcgc ccccatcgca tacgtgcagc cgggctcctt tggtgccgct gccggtgccg
     4261 tcaatgccat tctctcactg gcaaaactgg ccggcgtcgg ttttgtcatg aacggcatat
     4321 ccatctggcg caaatcactg ctggacgggc acaccgccct gagcgccagt gaatccatca
     4381 gtaaagggaa cgtcaagttt gtggccggcg ttctgctggt tttcaacgac cgggttatcg
     4441 atgccactct ggcaagcctc ggtatcgcct tctgacccct gtattttttc agtaaatcaa
     4501 tctgttaata aaggagacac tatgtcacga cttcgtgcca tggcggcgcg tctgcgcgct
     4561 gcatctgttt atgttcagtt atgcctgatg gcctgccgtg accggctggt acgcctgtta
     4621 ctgctgcttc ccgtgatgct gctgccccac accgcaatgg caaataccct cttcgatatg
     4681 attaaaaccg ttggcgatgg tgccagaggc tccagcagtt cgtttgtggc tatcggtcag
     4741 gttattggcc tggtgctctt tatcggcggc ctgcttggcc tcaaacaaat cggtaaaaaa
     4801 cccggtgcag ggctggcctg cctgctgtca attctggtcg gcgtctgcct tgccgtcgta
     4861 ccggaactga tgggcagcgg acagaaaacg ctcggtcttt ctccggtttc aatcagttaa
     4921 tccgcaaagg caccgggaaa ccggtgccgt cttcacacaa tgaggaggac cagaccatgt
     4981 cctgtgagct accccctttc accctgctca gaacagagca cggcaacgtg attgcccgcg
     5041 acaaccggga taatcaggag tttgtgtttg cagagtgtta ctccaccgct atagcagcac
     5101 tgattctggc tgccgtcacc ctgtatacag agagaaaagc gtatgaaaaa gaccccgcca
     5161 ctcatcgccc cggtccggga acgatggctt ttcccgctcc cgcaaccgga cacggtattc
     5221 agcggtgaag ccctgttacc accgccagtg ctctcaacag agggacgctg cccctgctgt
     5281 cgcaggaccg tcacccaccg cttcattctg gaagattcat ggccattaca gcagatggct
     5341 gacacctgcc gggatacggt cgtcctgctg gaaaaaaacc tcacccgggt gatgcgcctg
     5401 aaaaaacgtc cggtaccgga aaacgctgac gaaaaaaaga aacacacccg aaccctgcag
     5461 gatgcggagc ggtccctggc acaggcccgc ctgtcggcca ggcgactggc cctgcgtcat
     5521 gtggaaaagt cgcaaatcgt cacaacggat gcactcagcg aaaatgaaag tgacctgctg
     5581 cagccggaag gcccgccctt tcatctctgt gccttctgcc atgcctggca ctgtctgaac
     5641 ggctatgctg ccgcacaggg agtgatggtc tggctccccg atttgcatcc ggcctcggtg
     5701 gttgccctca atgcgcgggc actgcaggag attttcagcg ataaccgtca gcgggtcagg
     5761 cagggacggg cagtcctgaa tgccctggta cagaaccgtc tggccgttga ggagaaattc
     5821 cgcacctggc gtccggcgga ttttgctgat gccctgcgcc gctggccgcc ggcacagcgt
     5881 aaaacgttga gggagaaaat ggacggggtg gcgctcattc tgctgccgga ttcgtttcct
     5941 gacaaaaagt acgtcatgtg acacacaccg gtgccggtga cgttctgccg gccccaactg
     6001 acagaggaac aaaaaatgga taccctcact gccggatatc agttttccgc agatgcattc
     6061 cggcgcttta ttatccggac gctgactgac gatattccgc ctgacggaga tgaaaaagag
     6121 attgcccggt tcattctccg ccacccggca tttaacggcg gctggctgct ctttctggct
     6181 gcagaaattc atgaaagatc ccgtacccat aacagcatca ccgtcacagt gagcgtcgat
     6241 aactatctct catggatgga tattgccgtg tccggtctcc cggaattacc ggatatgccc
     6301 ttctgtgatt ttttcattcg ccgccagcag tttatttatc cgggtgtacc cccgacaaaa
     6361 tcaggagaag aatgatgaaa attcaccgca tatccccgga gacgcttata acactcatcc
     6421 acgcacacct ggccggaaaa gctgacagta ccgccaaaga agagcaccgg ctactccgtc
     6481 gtttcctgcg ggatgatgac ggcaggctgg ccggcgtact gctgaatatt gccggaatac
     6541 ttcagttcaa cagggaatta agtgcgcggc ataattatcc ggccacaccc ctgactgaat
     6601 tttcattaag gaaacgggga aaacaacttc atctctgtct ctgcagtctc cgtttctttt
     6661 atattcctcc ggtatttata caaaacaaaa gaagaaaatc tattgttgta caccttaata
     6721 agatcactta taaacaaacc cattcaatac gctgacgaag tgattcatac tgattatact
     6781 catctttgcc tatatgtttt ttaagcgtat taacaatatc atattgagga tgtcctgaat
     6841 tgtcagtata cagtgttgtt gcaggaagga ctgcataatc tgatatttca caacctgtat
     6901 acagttcttt gtcagttcgg caggaatggc aaagagaaag acatgtatac aggtctgtgt
     6961 tttctgcctc ctcaggagat aaccacgaca catattgcaa atcatctgta tcaggataac
     7021 aattttcttc ctgaacctga actctgaaaa tatagaaaaa taattcatct tttgaaaagc
     7081 atgctatttt attcccccaa tgcaccgcat tatcaagttg atccgataaa taaatccatc
     7141 ctggtgtcgt ctctaacgac cagcatataa cagaaggagc atcagttctt attttcccat
     7201 cttttattat ttgcaggcct ctgctcattg ttgtcgcatg aaaaagattt aatattttat
     7261 tttcagcctt cactctattt cctcatttcc caagaaacat attaaaaacg gaaaacatta
     7321 cacataacct ggaggacaaa tcatgacttt aaatttccct gccggggaaa ctacactatt
     7381 ctcagcaaaa aacatcctga cagcaatact cactcagcac aacttacacg catagcgttc
     7441 ccatttgaat acagttgcag cccgcgattt attttgtctt ccggagccac ctctgccatg
     7501 aatatcaacc gcattatttt tgccattgaa gactgtatca gtaccctgtc acggtattcc
     7561 gtttccgcat tcttccccga ttactgtgat ctgcacaccg tcatcggtct ggaccagcag
     7621 gaccgcgaac gccgcccctg gctgaaagcc ccctacattg ccaccacaaa acaggggtat
     7681 tacctttccg tctttgaaat cagcggtgcc ctgaaagaaa tggatgaaaa cccggaccag
     7741 acggcccccg ggacactgga gtcactgatt gcagcactgg ctgagcgaat gaacacggcc
     7801 tggaaaaatt ccggccacaa aatcagtttt gttttcgaac gcgatccgga gcgtggaagg
     7861 gatgaaatcg aagccatgct cttaccccag cgaaaatcca tcgcacgcac ggggatcctg
     7921 cttcaggacg tcctggatga aaaagtcacc acactcaccc cctggcttgt ccgggagcgc
     7981 tgctggctga ccgtctggag ctctgaagaa ctgctgagcc ggaccgatcg caaggaccat
     8041 cagacccgtg tcaggaaact agctgaacac gctccgccag cccgttttgc gcaggatccc
     8101 tggcgctgga cactttccgc cctcaaaata cggcatgacg ccttactgga cacgctggag
     8161 caggccctga cacatgattc tgatggcctg ctgattcgcc tgatggatat ccatgaagtg
     8221 ggacgggaaa tccgccgcca gctggagcgc aacagtacac cggcagtatg gcagcctcat
     8281 ctgcctgaag atgcccgtcc tgccggctgg cgacagggcg gggatacctc tgtattccac
     8341 gcaccgtcgc tgaatctgca gttattcacc acacagcctg aaacacacgg cagcctgatt
     8401 caggccggtg agctctggca cggtatggtc gccattaccc tgccccctca gaatctgcgg
     8461 acattcaacc agctggtcag ggatgtgccc cgtgcaatcc cctggcgtat ccggatggat
     8521 ctgatgcccg acggcatgaa ggcgctgggg gtcaagaaaa ccctgctgac ctacagcagc
     8581 tttattcctc ctctccggcc catgtatgac tcggtaatga tgctgaatga catcaacaag
     8641 catgatccgg tctgtatcat gaccattgtt gccaccacat ggggaaacag ccgggaagtc
     8701 tgtacccgta accaggctct cctgaaatct gcgctggaag gctggggggt atgcggcacc
     8761 accacaacct tcggtgaccc gagacgtgcc tgggtgaaca cggttctggc agcctcccac
     8821 agctccggcc cgatccccct ttacccgccc ctttcacacg ccctttccct gtttcccctg
     8881 aacagggcag gctctgtctg gcggggacag ggaaatctga tgatgcacac cgaagacggc
     8941 tctgcatggg aggtggcgct ggcgtcatca caacagaaca agcacacgga gctgacaccc
     9001 ggcgcacccg gtctgggcaa atcggtactc atcaacgcac tgtcggagat ccagatagcc
     9061 tcagcccaga agaatctccc tttcattgcc tatatcgaca agggattcag tgcacaggga
     9121 ctggtacagc ttatccgtga cagcctgcct gagcagagaa aggacgaagc cgtggggatc
     9181 attctgagta atgacccgga ccacacccgc aacctgtttg atgtgatgta cggggccaga
     9241 aaacccgtca caccggaaaa gaacttcatg gtctccgtcc tctgtgcatt gtgtgtggat
     9301 accggcaccg gtcagccctg taatcccggc gacacccggc aaatcatcag cagcctggtg
     9361 gacctggcct tccgggaata tggcgagaat aaccccagac tttaccgcgc aggtacggaa
     9421 cccctggtgg atctcgccct ggaagagagc ggcatcgctg aacaacatga tgccggatgg
     9481 tggaatgccg ccacctggtt tgaaatcagg gacatgctgc atattgcagg gaatatcccc
     9541 gccgcccagc gggcacacta ccaggccatg cccctgctgg ctgaaatgtc cgccctgctg
     9601 ggacagccgt ccatccggga tgtgttcggc accgttcagc gtgataactc ggaagagcgg
     9661 ttgctggact acatccgccg tgccctcgat cagggacaca gtgactaccc gatgatgtcc
     9721 ggctgtaccc ggtttatgct cagccccgac acccgcgtgg tcgccgttga tctgaacaat
     9781 gtggccgggg acaagacacc tgccggacgt ctgcgcaccg gtatcatgta tctgctggcc
     9841 gggcagatcg ccggcgggga ttttgtcctg ccgcagtatc aggaggaaat acggaaaaat
     9901 ctcgacccac gctaccatga ggtgattttc cgccgcattg aacagcttga tcaggaggtc
     9961 aagaccaaag tctatgacga actgcacaat gcaaaaggga tcaacttcat ctgggaagcc
    10021 ctggacaccc aggagctgga gcagcgtaag ttcgggatac gcacggtgct gagcacccag
    10081 ctgctgcagc attatcctcc ggccgtgctc aaatccgcca acaccctgtg gctgctgcgt
    10141 taccgccctg acgaaattcc gttcctgcgg gataactttg gcgtcccgga agtcacactg
    10201 cggcgcttcc tgaaaatgcc ggaaggggcc gcaccggacg gcagcggtgt cccggtactg
    10261 gctgtgttcc gtgtaaaaaa cggcacgctg gcccggatcc tgaaattcac cctcggtcca
    10321 ctggagctgt gggcactgaa ctcctctcct aaagacagcg ccctgcgacg tgcgctgacg
    10381 caggaggtgg ggtcactgcg ggcacgccag atcctggccg aacactttcc ccggggttca
    10441 gccacctcac tgatagaaca ccgcgcccgc acacatgaca gtgaaaacgt catccatgag
    10501 ctggcagcag aactcatccg taagcaggga tataacctct gatgtacagc attcatatca
    10561 ccccccgctg ccggcgtcct gcccggggac aggtcttcct gaaaacactg cgcgtggacg
    10621 gtgacagttt cagcttcacc ctgccgtccg cctgcttcac actgcgaccg tcccccaatc
    10681 cccgggtcat ttttgccagt gacagacggg ctgacgggga gatgagtggt attcgcctgg
    10741 ccctgcccgg agagacaggc acgctgataa caacggccac ctggcaggtt aacgtgccgg
    10801 agcgaccgcc ttttgatata cagcatgtca ttcactggct tctgcccgca ccactcactg
    10861 cgctgatgtc tgacaacacc tggctgtggc cggatgtccc cggcacaccg gcactcacag
    10921 ccggagactg gccggtcatg gaaagcgaca ccccgacact gaaacgcgtg aagcgtcgta
    10981 ccttcacgct catcgataac cgtaaccgtc agggatggat cacgcgccgc ttccagtgtt
    11041 tcccgctggc tccgctgccg gaatcactca gtcatcgcgt accagacatc ctgtatcccc
    11101 cgaaaacgga gaaaaaagaa tgcaaagaca atctgcaacc acactgcttg cactccttgt
    11161 aggctgtgcg gttatccctg ccacccatgc ctactctgtg aatgttgtgt ccagtcagcc
    11221 ggtcacccga cagatcatgc cggagctggc caccatacag ggcatcatgg aggaaatggt
    11281 ccgtatgcag acggccaccg gcacagccat cacccagaac tcagaaaaac tggccaccgt
    11341 cattgcccag gacgggcagg ccacccgtca gcagatgata ttcagtaatg aaacacaccg
    11401 cctcgaggag gccaggaaaa gcttcagcgt gccggactcc atctgcagtg aatccgcgtc
    11461 cggcattgca gcagaaagcc gccgggcagc agcgtctgca gcggcacgtc tgagtcaggg
    11521 gggaggggtc agcagtaaac ccatccggga acgtctctcc cgggctgcgg actccccggt
    11581 ccgcgaggcg tatgatagtg ccggcatcca cgccggttac tgcacggaag cggagtatgc
    11641 ccgctttggc ggaactgatg tctgccctgc cgtgggcgat ctgccgggcg gagacagcca
    11701 ggtacgatcg ctctatcagg gcgcaggcac agcagatacc ccggccgctc tcacctggga
    11761 tcagaaacag attgatgccg cgacggctta tatgaaaaac accgccagac cgtcagcggg
    11821 tcgtgccccg gggaaaggag aagtgggcac acagaccggg cgcacgtatg tgggtctgca
    11881 gaatgaatac aacggcatca tcgatgccgc ctctcatccg cagctgtcac tgattgctga
    11941 cagtacccct aatgaagcca cccgtggggc actaactgaa gccctgcagt caccgtcagc
    12001 tgcggcatat tttgaccgga ccgcatcatc agaagcccgc acccgtggcc acatgagcca
    12061 gcgggagttt gaggcctttg aagccggcag acggtatgcc aacaccgact ggcagcagga
    12121 tctgcagggc atggaaggcg ataaccttct ccgtgagctg ctgcgcacca ccgccctgct
    12181 gaactggcag atgaacgatc tgaaagagca gattcgtcag ggtaacgtta tcgccgggca
    12241 gcaacttgcg ctggccgcac ggcagtatta cggacagcgc cttggcgaac tgtcacaggc
    12301 catgggccag gggagcgtaa gatgaaagac gacaacagca tcgacgaaaa aaaaccggcc
    12361 ccccgtctcc gccgcatcgg tgatataggg ataaatctgt tcgcaccact ggccgaaacc
    12421 cgctggatgc tgcgcagctg ccgccatatg ctggaccagc ataaaacgcg cctgaagcgc
    12481 ctcatgcccg accggcaacc ggagagtccg gtctgtatga cctgggagga agcggtcagg
    12541 gccagcggac tgacggtcga cgaactggac cggcgtctgc gacgtcgcag gatgttctgg
    12601 cggacaggat tctggctgat gatgataccg gtacttctgc tgtgtggtat ggcccttgcg
    12661 gccaccgcac tgccggtcat caccctgata cgtatcctgg tctgcctgtt actgatgagt
    12721 accattgcag cactgtgtgc ctcaaaagcc ctgattgcgt cataccgcct ctggcagctg
    12781 aatgaacgtc gggtcagcga ggacgaacag ggcacattcc ccgattacct gcaggcccgg
    12841 cacggctgga aacaggccac atggctggcc attacccacc ggcagtactg actgtccggt
    12901 ctgcaatttc ttttcctcat tccgggggct gatgcccggg catccccttc ggttccggag
    12961 ttaccgtgaa aatcataccc agagcactga gtgcaggtct ggcgctggct gccgtacctg
    13021 cacaggcttt cgtcacctat caggacattg tgagtgccgc caccaatccg gatgacctgt
    13081 cgcgccaggc actggtcacc atttttggtg atgtggtcac aaatcccctg acagccagca
    13141 gcaacaccat cattggcaac ctgttcatgg tgtttaacgc aattatcgcc acgatgggga
    13201 tagtctggtt tctgtttatt ggtatccgtc atgctgtccg cacgggacac cagggcagtg
    13261 tctttaacaa cggacgggac gtcgtcggca tactcagtgt cgtgagtgga tttttgatga
    13321 ttgtgcccac cgcttccggc tggagtctgg cgcaactggt catgctgtgg ggcgcatcca
    13381 tcatgggcat cggttccgcc aatatcatgg ttcagacagc ggcagaaaac attgctgaag
    13441 gctattcaat gaccgtacag ccagtacatg tctccacccg cacggcagca cggggaattc
    13501 tggagatgga actgtgcaaa cacgccatga atacgggcct cgatgacttt aaccgtaccg
    13561 cacgttcatc aacgccgccc atgagcgaat cctcacggac agagaatggc agatataccg
    13621 tcaccgtctc aaatggcagc gccacctgcg ggagtgtgag tctctccgtg aaaggaaacg
    13681 gcagtaacaa gcagtccgcg ctggcccatt ttttcaatcc cttcattgaa acggaatatc
    13741 agggggttct gacggcacaa cgctccgcca tggataccat gctccgtgac ctggacagcg
    13801 cggcgcagga atttgtgtcg gcataccgga cccggcgcga cacgggtaac ggcaacctgc
    13861 cggatattga aagccgtatt cagaaggcag ccaaccggta tgaagaaacg gtgcagaagg
    13921 cgttgccggc agataacgca gaaactgagc gcagggagca gctgaaatcc tacctgacca
    13981 cctacggctg ggttgccctg ggtgcctggt accagacctt tgccaccgcc aaccagcgac
    14041 tggaagacct ggccaaccgc gctcctactg tgagtggcgt ctcgtcactg ggcgaagccg
    14101 gggatacgga attcctgaag gaagtcaagg gggcttacca ggcacagcta cagaactcag
    14161 cgtatactcc tgcactggga acagccacct ctgccagtga ataccacgca tctaaaactc
    14221 aagaccctca atcagcaatc ttaaaattga ttgggtttaa cctaacaaga atgactaact
    14281 ggacagccac ttatctgagc ggtacaggta ccacaactaa tcaactaaat ccgttgataa
    14341 aaatgaaaaa cattggcgat tacaccatgg ttacgaccga aaccatctgg gcgggttata
    14401 ccaccgtcag ggtactttcc acggccggca gcaactccgt ttttggtcga ctggtcaatg
    14461 ccgtcagcgg attatctgac gccgcaaagg cttttctgga agcatccgca ccaccactct
    14521 acttcctgct gtttttgctg ttttgtgccg gtttttcgtt atccatttac ctgccgttta
    14581 tcccgtttat cttctggatg accggcatcg gcaactggat agtcagcgta ctgattggat
    14641 gtgctgctgg ccccctgtgg gccgccaccc atctgggaac aagtcaggac cggggaagcc
    14701 gggccgctta cggttacatc tacctgatag acagcatgat cagaccgctg ctgatggtgt
    14761 tcgggttctt ttttgcatca gtcgccatcg tggccggcgg cacaatcctg aatgcgctgt
    14821 atgctgctgc actgatgaat gtccagataa actctgtgac gggcgtgttc agcatggcgg
    14881 gatttttgat gatttatgcc cgcgcctgta ccacactggt tgcagccgtt tttgcgcttc
    14941 aggcttatct gcctgaccat gtaattaatt ttctgggcgg acgtgacggt gttaatatgc
    15001 tggggaatat ggtttcttca gtcaaagata ttattgccgg aggtaatacc aatatgcgca
    15061 gaacacctgg cgttcgtgaa gaaaaactga aaaatattaa tagagatgat aaagacggaa
    15121 taaaacaata aatgaaaata tcgaaaaacc actaaaacac cattgccata ttcacctgaa
    15181 gagattaaaa tacattcagg tgaatatgga ggtttattat gaacacaaca aaaagcagat
    15241 atccttcctg gtgggcatat tattacctta tccgcactgc ttattttata tttggtgttc
    15301 cttttttatt actatgttct ctaggattta ccggtatgat gcttacagaa ccatatatag
    15361 aaacagatat tcaagcatat ctatatactt atggcagttg gctcctatta atactcccat
    15421 cactctggct atatatgaaa accagagcga agaaaaagaa agtccagcag gttgttcagc
    15481 agataaaaga aagcagtccc tttgcgccga ccagcgatta tgagcaactg tcattctcga
    15541 agagttgtta tttcgggatc gacattaaaa acggcaccat gctgtatgtg cgtatttatc
    15601 caaacaatgt gatggatgta attggtctgg atattcacaa ctttacccgc acggttgctg
    15661 aggacggcaa gctggaaatc cacaccacct atgtgagtct gccgatgatt cctcttgaga
    15721 tctccggcat ctcatcaagg acgctggcca acaccatgca caccatggct gcacgtggat
    15781 atgagtacaa ggaacgtttc ccacagatga tccggtatcg ggttaaagag tgggaaaaag
    15841 ttgccggtgt tcctgttgca gaagtatttt gaggtagcta tcaactgtta gacgataatc
    15901 cgtcagccgg ttttctctga accggctttt tcttttcagg cagaatgcct gtctgccgtc
    15961 tatctcagga cgccgcatgg agtctctatg ttttccccac aaattcttag cactgtcttg
    16021 cccactgtag acaaaatcaa tacaattctg catatcagtt atctataaaa gataacaata
    16081 tcattataaa taattatcat atccacacac tcaaggcagt acctagaaaa cataaagata
    16141 aaatcatgtc tgaatcccgc agcaattttc cagcacgaca tggtactcca tggaatactg
    16201 aagagttaca gcgtcttctc gtatccatag cggagggcgt ctcgctaaaa aaacttactc
    16261 aaacacatca acgatcgtcc gggggaatac gtggagcaat caaacgcctg cttccttctg
    16321 agtttcttga gttcaacgat ataaacacgg tgaatgatct cagcaattac ttcaacaaga
    16381 ttcctcgcca agaacacaat agtatcatca ataatttaat atcattgagc actgcaaaaa
    16441 caattactca tgataaagtt gaaatacaaa agaatagttt taaacaaaat accccactcc
    16501 ccctctgcca acccaataca gattttaaag attatatagc cgatgaacat gatgtcatta
    16561 tgctaattca ttctgctatc gaaagtttat ctaataaacg acatcaatat attcttctaa
    16621 tgcgactcgg cacagatgat tcacctcatt ctctggcaga tattgccgtc aaattaggaa
    16681 tttcccgtga aagggtaaga caacttcagg aacgagccct tggcttacta gctgctaaat
    16741 ctcgctatga agggacacct ggagcttgcc ttaaagccct tattaactca ataggtaatt
    16801 caccatatga tatagcagta tggatttatc gtattgttca tcgggatttc gttacatcct
    16861 ctgagttagc cgccaaattc atcatttttg ccgcaggcat tcctaaagca agacgagatg
    16921 aaataaaaaa ttcgctctca ttaatatcac aattacaaaa gcagaagtta cgtgaacgaa
    16981 gagcagaatt agcacacgct cacgctcagg aaagagcccg aatcaagaaa gaaagagttg
    17041 agttcatatt cagtaaatgg ctagacaata cacattggcc aaaatatatc gcacctcctc
    17101 cccccgtaga acagctatgt tcacagagag cggttaatga tactgacata tctggattct
    17161 tttactctca aaaattaggt cgtacagtcc agtacgaatc agggcttgag tttaaaataa
    17221 tgaatctatt agaatgttgc gatcaggttc ttttctatca agaacaacca ttttctattc
    17281 catattgttt taaaaacaaa tcaaaaaaat actatccaga tttactcatt gcgacagttg
    17341 atggtcggtg cctacttatg gaagtgaagc caacagaccg aatgaccttg agtctcacga
    17401 gaataaaaag taatgcaggt cgcaaatggg cacatactcg aggatgggga tggttggtca
    17461 ttagtgatag gttctcgtta cgtcagctgg aagaacatgc catctcaaca aacacatgga
    17521 aacttataga ggctgaactg aaaacatcaa ggattttagc atggcgtgaa atgatggagt
    17581 tacgtacacg atacgagatt catcgtttag atcttattgc atatatcatt cagtctggtg
    17641 ctgaagtgga taacttttat cgaattaccc cgaaaatatc taataataca tctaaccaga
    17701 gacaaccaaa tgagagcgac tgtcataatt gatgacgcac aggacggaaa aaataatagc
    17761 tgccggacag cagcaaccag cagtcgtccg gtaaaaaaga caaacaaggc gcattgaggt
    17821 cagccttcgc aacaatccgg cggcagatgt cctgccgccg tgttgcccgt taacgtttaa
    17881 cttcgtaggc taacgttgcc acaaacaccg tatttccctg ctcgatacga aatccgcaaa
    17941 gcgaatccct caccatccag acaaaacaca gtatcgtcac acagacgacg attaacatca
    18001 ttaaaaacgt tcgttgtggc atcgaagccc ctccattgcc tttcggccgt catcgggcta
    18061 ctataatgtt gtctaggcat tacagtgggc cctgggttga ttgataaaaa aatcgcctgg
    18121 ggctttctgc tacatgccgt ccacaaaacg ctttcggcat acagcctcaa gcgcccgccg
    18181 ctattctaat cgttttcctg tccgccacaa tccttttcat gccaccacgt attttgtgtc
    18241 agggacagaa ataaagccgg aaagtgacta cgtgtgcaca catctgtttt ccgggctgca
    18301 ggcatcgtgt tattttctga aatgcaaaat aataaaaaac aaggacacat catgaaggat
    18361 gaaatacaca gtaccagcat taacccggat gttgttgagc agtttctggc ccttatgaat
    18421 acacattgta aatcctcaga agaggcgctc accatgctga gtacgctttg ttacaacctg
    18481 atgatgacat gcggtatcca ttccatggac agccagatgc tcaatgggca tgggatatcc
    18541 atgcaacttc aggggattga tattgccgaa aagccaggca agaatattca ctaacctgaa
    18601 cagggcctga aaacgctgtc ttttcaggcc tctgcaggca aaaactgatg acaacaaaat
    18661 ttctgcgttt gtaccccacc gatgaaaaag acaaaaactc acaccgggct tctcatcatt
    18721 aaagataaaa cccgacgtgt cagcctctac gaaacgccaa cggcatggtg tatcaggggg
    18781 caggagtgct acagcaaaag cactggacgc cgctgtggca gccacgacag tctgagcaga
    18841 ctgcgcctgg acagcattaa gcctgttgaa tgagtggtgg caccatgaaa gataacaaca
    18901 caaccgccac cccagaaacc tggaaaatgc gttttctggc caacgggaca tccggcacat
    18961 ggttttacac tgacgacatt gcagtcactg acgcgtggct tcgcgcgcac ccggatgatg
    19021 tggaggttat ccctctctgc cggatacagc cccgccagca ttcccatgaa caacatattg
    19081 aagaactccg cgcagccctg gcccggatgc tccatatgca tcatatgatg ctggaaacca
    19141 cacattaccg ggaaaatatc accgatgccc gttgtatccg tgaaatgcat gaagcgccat
    19201 tacaggcgct caatattctg tccagatacc cgcataaaaa ggacgaggtg tgacgcatgt
    19261 ggctactgat tttctggctt tcatcgccct ttaatgtctc ttccggtgat gcccgtcagc
    19321 ccacactggc ggtgcacagc caggcctttc ataccgaacg ggcctgccgc gatgcattgc
    19381 gcgccattaa aaaggagaat gttggcgaat tttctctgcg gggcgtttgt gtttctgacg
    19441 gaaatacgaa ggaggaggct gagagctcat gacggatatt actgacagac atacacttcg
    19501 ccgtatgtcc tggtcggaac tcatcacggc aggacaggaa gcagaatttc agcatgatta
    19561 cgagcgtgcc agaatagtgt ggtcattcgc cttacatgtt gcaacaacca cgattaacaa
    19621 aaatctggcc atagctcata ttcgacgctg cgatactttg ttgcataaat caaaggctgt
    19681 acccggaaac aacccgtgtg gttccggtca gcgagtctga gacctcaaca tcccgggtga
    19741 tgattgtcat tccggactga cgcagccgct gcacatctgc ggcaatacgt tgaataccaa
    19801 gccagaacag accatccagc gcggtgacaa ttaacccgct ttccagcgcc agtttcagcc
    19861 gtgctgtcgg ggctttaacc tgcccggcta tatcagtgaa acaacgcgtc gtcatgccat
    19921 agctgtccgc ccgccgtatt cgcgtcgcaa gactgtaact gaagctatcc ggcacgccgg
    19981 ataagtcatt ctgcagactg tatacacatc cccgctgccg ctcattgaat acgacttttt
    20041 tctggctgag ctgccattcc cgccgcacgc tgtcaatcat ctgcggtgtc cgggttaaca
    20101 gcagacgaaa cggcagttca aaactggtca cataatcaac attaagtaag gcaatccgca
    20161 gccgctccct ggctcctgct ccggtatcat ggcattcacg catgacatct tcccactggc
    20221 gcatcagtcg cggtgactca ttcctggccc tgaactgata tttctcaatc tgataatcaa
    20281 tatgttcctg cataccgtca ccggatatct gttgccgtat catcacggat atcagcgtac
    20341 tggaattcat gccctgcgtg aagtatcaca ctgccaaaaa aaacacctga acgttattat
    20401 ttcgcaatat taattgcacc tgttttcata aataaaaagg agacgatgaa atgataaaat
    20461 atatcttttc actcaccatt attttttccg cttctctctg tgccgcacag attcaggggc
    20521 acgtcatccg cgttcttgat ggtgatacgc tggaaatatt acaggacaaa aagcctgtca
    20581 gggtccgtct ggctaatatt gatgcaccgg aaaagaaaca gccctttgga cgctggtcag
    20641 ccactcagct gaaggacctg gtggccggaa agcccgtaac cgtgcaatac gcgcagacag
    20701 acaggtatgg ccgtattctc ggccgggtct ataccgctga cggcacagaa gcaaatcgct
    20761 ggcaggtccg taacggcgct gcctgggtgt acccggacta caataatgac tatactctcc
    20821 cgccgctcca gcgtgatgcc cgcgccctga agcggggatt atgggcagat aaaaatccgg
    20881 taccaccatg gacctggaga aaacagaata agaaataatt atttgctgat atgaagaaaa
    20941 aaaccatttc aaagaaaata aatggcaaaa atacagcatg gaaaaaattg ccatttttac
    21001 cgccgcgtta tttttaatat ttgctgtctg ctggtcgtaa taagcagaat aacagaaagg
    21061 tgtaaaaatg aacctggttg atgcatttgt cactaaagtg atatccggac cgtatgaaga
    21121 atacggaaaa tggtgggtgg atgtagaata tatttcctgg ggagttccgg gtaaaagccg
    21181 gctcatgttt gactcacggg aacaggcaca ggaagtgcag gaaggttttc gatttttaac
    21241 ctgagcatat tttgttaaag ggcagaaggc ggtgcccctc tgcccttaag tacgtttccg
    21301 ggattataag tcaaccagtt cccggatcag ttccaggaga gctatcactg cgttagccac
    21361 agcaacaacc gccctgagaa cgatgattaa caggttaatc atccgcttct ccatcaatgg
    21421 agaaagaaca ccgtggtttg cccttacatt gcctgtcgcc acgttttcta tagtgtcagt
    21481 tatagacagt tcttctccgg ccagagcacc aaaaccagca ccacctgatg ttttggccag
    21541 gacttgcaac ctgttcatcc cggtgaacga tatgccggaa agaacgtgcc tcaaagctga
    21601 cacaccgagt atatacttat actgtgcttg cagaaatatt atttcagccg tattatttca
    21661 tagtcagtta taggcagtta tcttccggtc agggctacca acttcgatcc ggaatcgcaa
    21721 aaaaaccggc ctcaccagtc ggtttttttg cgtctgtacc tcctccccgg acagcagttc
    21781 tctcactccc gttgaatatt tcctgttgaa ttttacgaaa atacggcaaa tggattgatg
    21841 cgaaggtaag tctccagaca aggctaactt gctgattata cttgtttcag gcgatatcgc
    21901 tactcccgga acaatagact gtaacgaaca gattgcgatc aggtgaatat aacgatattc
    21961 tggtaacgtt agttgaaaag agacttcctc cgggccagag ccacccactc tccctgggat
    22021 cgcaaaaaag ccggcctcac cagtcggctt ttttgtgcct gtctctcacc accttctgct
    22081 gttcctgtga aaatgccctg ctgaattcag ccctgcccgg tcattaccgg cgattgttcg
    22141 cattaattcc actcagcctc atcccgaaat ttatctattt atcgatggag gaatcctcgt
    22201 gtcttaccaa cactccccca caacagaaga cccgatgaac atatggtata tcgttggcac
    22261 catatgtctg ctctttgcgc ttctcatctg gctcttttta cccgacatcg tctttgccac
    22321 ctgcctgatc ctgcacacaa tgtgggggat gactgactgg gggccttttc accattttgc
    22381 cgccccccgt tataacctgc tggccaacac cgccaacaac gctgcaacga tcactttcag
    22441 ccagtggctg gatgtcatgt cacagaccgt ggggatcctc tggctgattc tgcttcccat
    22501 gacgatcggc tttctgtgga tgtggtttca gcatccggcc cagccccgct tcacccgcag
    22561 accactgaat atccacacgc tgccacatct gttttcatca ctgtcaccgg cgatcgcccc
    22621 cgttctggct gacggagaca gcaacagact cttccacgga caaaaacgtc ccgagcgccg
    22681 tgtcgctcta acccccgaag cctttgtgga gcagaacaac cttatccgca acatgcagct
    22741 cgatgttgcg gccacgcgcc agtgctttat ggcgcaactg ggccagcctc tgaccacctg
    22801 gaaagacatg gctccacatg aaaaagcgct ctttgccgtc tttggcctgc agttcttcct
    22861 tggggaccgg aaatcggctg tttccctgat gaataacctc aacctctcct gcagactcaa
    22921 aagcaagcgg gaccaggggc gtttcagcac gccagtgtat tcactggcca gaaacgcctt
    22981 tatccgggtc ataaaaacag aagacgcaca acagtggctc aggcagcacc gttacgttcg
    23041 cagcggtctt gtctggcttt acgcacatga tttgcgcctg acgcccccca actggctgtg
    23101 gctcaagggc gttgaccgga cgctcttcta tgcccttcac cgggcaaaca ccacaaaagg
    23161 cttcatcgaa ggggccggcg tggtggccgt tgcccgggca gaaaatgaag ccagccgtct
    23221 cggcctcccc tgcccggaac cctgtgtgga agaggccatt gaagggcttc gacgggacat
    23281 gctggggctg ggcctgatat gggacgaacc tcagccagat cgcgatcgta aacgtcagat
    23341 caggacccgc tggtcactca cggatgacgt gataccccgc cgccatgata acgacgaaga
    23401 cactgacact ggtgaaacca ccgaaaccac cgaaaccacc gaaaccaccg aaaccaccga
    23461 aaaacagcat cccgccgaca aggaaaaaga acaatgacca tccactacac tgcaacactg
    23521 aataataaac acctgtccct gaccgccctg taccctgacg gtaatcggga agtgattgcc
    23581 atcctgccag tcacagaact gggcgcactg attaaccgta ataacaggct gatgcacctg
    23641 tccttcagtt ctccttttcg cgaaataacc gaaacggtgc ttgttgatac cgttgatgca
    23701 gaacgtcccg gacatcacat cggcagaatg ctctcagcga ttgatgcctg cctggaggcc
    23761 gaatatgccg gaaaaggaaa acatccctcc cggaaaaatg gcttctggcg gacactgatg
    23821 tgtggcttta tcgccggaat tgctctgccg gcgacagttg caggttacca ctggatgaag
    23881 gcagagcccc cttccacgct ggcttaccct acatctcagg aatggagagc tgaaccagta
    23941 accgacacag agacaccacg cccacatacg gaacatcatg ttaacagcat cccggcccgc
    24001 ccggctccca cacagcagga accttcttct gcccccgccc cgacggcagt gacggaagaa
    24061 caacacgttc aggccgctga ggcccgccgt aatctggcca cagtacttaa acgcaacgca
    24121 gaccggggga tgttcaccat taacctgtcc actggtcatg aacgcacgct gtacgccttc
    24181 cttgaccccg catgcatcaa ctgccgtctg ctggaacccg cgcttaagca actggcccgg
    24241 gaattcagcg tggtgattta tccggtctcc gtgattggcg gggagtccag tacaaagcgg
    24301 actgctccgc tgctgtgtga accggactca caaaaacggg cgaccggatg gcatcagctg
    24361 tattccgctg acagtggtat ggcccccacc ggcgatgcac cggccgcaca ggatggacag
    24421 tgtctgaccg ccgcacgtgc tgccatcgat gtcaataacc aggctttccg ccagttcggt
    24481 tttggcggaa ccccctgggt gttatcagat accggctggc acctgccgtc cggcatcctg
    24541 aaagacgcca cttccgttcg cctcttcctg aaagcaacag atagcgagga gtatcacaat
    24601 gagccagcac cacgttaatc ctgacctcat acaccgcacg gcctggggaa acccgttgtg
    24661 gaatgcactg cataacctga acatcactgg tctgtgtctg gccggcagta tcattacggc
    24721 gctgatatgg ccactggccc ttcctgtctg cctgctgttc accctggtca cctccgtgat
    24781 attcatgctg caacgctggc gatgccccct gcgtatgccc atgacactca gccttgatga
    24841 cccctcccag gacagaaagg tcaggagaag cctcttcagc ttctggccca ccctgtttca
    24901 gtatgaagcg gatgaaacat tccccgcccg ggggattttt tatgtcggtt accgccgtat
    24961 caacgatatc ggtcgtgagc tctggctgag catggatgac ctcacccggc atgtcatgtt
    25021 ttttgccacc accggtggcg gtaaaacaga aaccaccttt gcctggctgc tcaacccgct
    25081 ctgctgggga agaggcttta cctttgttga cggaaaggca cagaacgata ccacgaggac
    25141 aatctggtat ctctcccgac gttttggcag ggaggatgat atcgaagtca tcaacttcat
    25201 gaacggcgga aaatcccgca gtgaaatcat tcagagcggc gaaaaatccc gcccacagtc
    25261 aaacacctgg aacccgttcg cattcagcac cgaagccttc accgccgaaa ccatgcagtc
    25321 catgctgccc cagaacgttc aggggggaga atggcagtca cgggcgatag ccatgaacaa
    25381 agccctggtc tttggcacaa aattctggtg cgtccgggaa agaaaaacca tgtccctgca
    25441 gatgttacgg gaacatatga cgctggaagg gatggcaaaa ctctattgcc gtggccttga
    25501 tgaccagtgg ccggaagaag ctatcacacc actgcgaaac tacctgcagg atgtcccggg
    25561 attcgatatg tcactggtga gaacgccgtc agcctggact gaggagcccc gtaaacagca
    25621 tgcttatctc tccggtcagt tctcggaaac attcacaacc tttgccgaaa ccttcgggga
    25681 cgtctttgcg gcagatgccg gcgacatcga tattcgcgac agtatccaca gcgaccgaat
    25741 tctgattgtg atgatccccg cactggacac atcagcccat accacgtcgg cactcggtcg
    25801 tatgttcatc acccagcaga gcatgatcct cgcccgtgac ctgggctacc gcctggaagg
    25861 cacagacgca cagaccctgg aagtcaaaaa atacaaaggc cgcttcccct atatctgtat
    25921 ccttgacgaa gtcggtgcct actacacgga ccgcattgca gtggaggcca cccaggtacg
    25981 ttcactggag ttctccctga taatgacggc gcaggatcag gagcgaatcg aagggcagac
    26041 ttctgccacc aacaccgcca cgctgatgca gaacgccggc accaaattcg ccggacgcat
    26101 tgtcagtgac gacaaaacag caaggacggt gaaaaatgcc gccggtgaag aggcccgcgc
    26161 cagaatgggc agcctgcagc gccacgatgg tgtgatgggt gagtcctggg tggacggtaa
    26221 ccagatcaca atccagatgg aaagcaaaat caacgtgcag gatttgatca gactgaatgc
    26281 cggtgagttc tttaccgttt tccaggggga tgtggtgccg tcagcctctg tgtatattcc
    26341 ggacagcgag aaaagttgcg acagtgatcc agtggtcatc aaccgttata tctccgttga
    26401 agccccccga ctggagcgtc tccgccgtct tgtccccagg acagtgcagc gcaggctccc
    26461 cacaccggag catgtcagca gcattatcgg tgtgctgaca gaaaaaacct cccggaaacg
    26521 tcgtaaaaaa cacacagagc cttaccgaat catcgatacc ttccaggacc agcttaaaag
    26581 aacacaaaca tcgctggatc tgcttccgca atatgataca gatatcgaat cccgggcaaa
    26641 cgaactgtgg aaaaaggctg tgcacactat caataacaca accagggaag aacgccgggt
    26701 atgttatatc acactgaatc gtccggaaga ggaacactct ggtcctgaag atataccttc
    26761 agtgaaggcg attctgaata ctctgctccc cctggagatg ctgttacctg taccgaatct
    26821 aactgcatcc ccgccccata aaaaaaatgt cgctcagaca ccatcaggtg gaaaacaaga
    26881 gagtcgtaaa aaaagattct gataacacct gtatggctga ttttaataac aacctttacg
    26941 cgattattaa tctcctccgc tcaccatagt aagcggagga ttcaggattt caatccatca
    27001 ccagaggtac aataaatgtg tattagtagc ccggaaacaa actcatggtc tgttatttat
    27061 cgtaaaaatt ccggagaaga tatcaatatc acttccctga cgtttaaaaa tagtcttctg
    27121 gcagcccgga cactgatggt gcccgaaaac tatatgatat gcatactcag aaatggggag
    27181 agggtcagac gatgggacag agaaatactt gcaggctcaa acagatggta caagtgttct
    27241 cccgataatt tcgaaatact gggtaagctc cccataataa ataaagtcac aacactgata
    27301 aaatcataat ctacggattt taatcagtta agctaaatca ggatgaacgc tttgattatg
    27361 aattatacat gaaaacaaat atgggcagca caaaaaatgg tgccagggtt ccccacgttc
    27421 gtaatagatt cacttctatg gatattggta tgaattccca gatcctgcca gacatgctga
    27481 ttaattttgc ccttattaac atcacagaca gaaagaacga gggtaccaat accattgatg
    27541 gtaactggca ggccgatgaa agacgccgtt accgggataa tgtaagaatc tatttttgag
    27601 tatgtcaaca taacctgagg caaagtacct caggttccag tgtagtaatc agccaacctg
    27661 acagtcttct gttagcccta cagatatgtc gtggagctaa ccgcgcatgt caaagccgga
    27721 tttttgaaaa tctctataaa cttccggatt gttgaaggcc gggaatttag ctttatgggc
    27781 tgctgacttt atcgcttcgc aataggcttt atcgccatta ctggttgata tttttaacgc
    27841 cgtgccatcc cgggagaatt ccatatgcaa cctgcatttt ttccttttcc atttttgcgg
    27901 atcatcaagt ttagcattaa ttgcatccct gattccccgt gcctgctccc cccattcatc
    27961 ctgatcatcc caacgtcctg aactgcaact acccagggct gtagttttat ggcaatttga
    28021 aggatgtaat ggcgcacatc ccgccacaaa gctgactaaa atagccagca taacgatttt
    28081 ctttaccccc atatattgct cctcaatcca tatagatctc agtaccatag ctctgtcaga
    28141 gcaaagaaaa tcttaactaa tacaagttaa gatttttaat atccactcct catccctaca
    28201 ttccaggtga tggtggcgtc cactcattat cctgaactgg agcataagat gcgccatgcc
    28261 ccccggatat gcttttctgt tgcctgatat attcgtggta gtactgttca ctacgctgac
    28321 gatgtaaatc cacatccggg cggctgatta caaagtgtcc acttccggtt attccagctg
    28381 cattatgcca ggccaccatt tccgcaaata catcattaaa ctgttgatat gagccttccg
    28441 gttcaaaacc cattcttggc tcacgatcaa acaatacagg agcaatcaga ttcagcttat
    28501 ccaccagttc atttctgtcc tgatgatggt agaacaccac acggtcccca taatccacac
    28561 agactaactc gccattcctc cgatcacgaa aacgcacact gccatctttc tgcagacggg
    28621 cttcgagaac gccagtgtct tcataaagtg gagttcctcc cggttcacag agaatcacgc
    28681 agcggtcggc attcgttgtt cgtcgcttgt ctttgcgacg atcacgataa tcccagccac
    28741 gtaactgtga taatgcagct ctgtctccct gtaccgcctg ttgctccacc cactgccggt
    28801 aggacaaagg ataccactta ccctccgcaa tcagtgacaa ccgctcttcc ttcacactct
    28861 ccttcagcct gatcagcgcc tgcatacgct gcacttctgc aatatggtaa tgcagcttac
    28921 gcaattgtgg atcccggaac tgcacacgga tatacgcctt gcgccgacgg caggcagcat
    28981 gaatttcccg taaccgttct ccgtaacgta agtcgggttt acgccagtgc tccttccatg
    29041 caagataacg cgcgcgcaaa tcttcccgcg cagctgcacg ggcttcacgg cgttcacgac
    29101 gcagtacagg atcgcgccgg aagccgagct ccacttcatc gctggcagca agttccggat
    29161 tgtagcgaca ttcaggcttc acccgttcaa atatatctgc acctgcaatc tcaaatggcc
    29221 ctgcctgcgg ctcagccctg gataacgtca aatccgggtg gatactgctg gccttcactg
    29281 gtgtgagctc atgattaaac gcatccacaa tcacaagacc atgatgctgt ttctgcaaca
    29341 taagcccttt acgcgcaaac atctcatggc acgcctgcca gctgaaattc ccgcccgcga
    29401 tcatatcttc aatcaacgac tcacactgaa cagtcgtgta cccccacaga ctttccttat
    29461 cggaaaacag ttcgcgcttt attgctccct gtggacctgt ccccatccga atacgatacc
    29521 attcgtaatc actgacagcc tgagtaccaa gattacgtcc ccggaatcgt tctccgggta
    29581 taacctgagt aaaaatatcc ttcggtacag gctgccatcc accatccaga tcctgcatat
    29641 aatccagcgt cattaacggc cacacactgt ctgcacgtac tggtgtctgt tgtttatcaa
    29701 atccatcatg cagtaccaga tgcccgtgct ggatccccat ccataatcca gcctttgcca
    29761 gagttctgtg cacatccagg caactttcca gctcaccatt tctcgccatt tccggtaagc
    29821 gttcggcaaa atgacggcag gcgtactgtt tcagactctc cgtttctgcc actttttccg
    29881 gccggatatc aacgtttatg gcttccggat cataacgacc gggtgtcccc acctgactga
    29941 aaatatctgt cggtacaggc tgatattccc ccagtttccg ccccagtttt tcagctgtcc
    30001 atgacggtcc gaacgaactg agtgcaacac cttcccgcgc tctgtcccag ccatccttca
    30061 ccaccagttc cccttcctgc atcgtgatat acagaccatc actggccagc cgtttatgca
    30121 aagacaacca gtcttgtgcg ggctcttcac gcagcccggc aatcgacatc tgcgcaatat
    30181 attccctgaa cgtctgcttt ttcccgcgtc gccaggcatt acggcgctca cgtaccagcg
    30241 ctgttttgcg cacaatacgg ttgccgggtg catgcacaaa gcagccatta tcaggcgcga
    30301 atccgtgctt aagttcaagt tcacgacatg cacggctgag tttttcctgg ctccaggaaa
    30361 gccagttaat ataaccagtt tcaggatgta cacgattcac ggcaacatgc acatgcagat
    30421 tatccgtgtc cgtatgaacg gcagccacgt actgatgttt tgagagttca agagatttca
    30481 gtgtgtgacg cacacagtca aaaagctgct caggacgcgg gctttcatgc gcaggccagg
    30541 aaagaatgta gtggaaaacg gggtctgtat cattacggga aaaaacagct ttgtccgcct
    30601 tgtactgcat ctcttcagca gctgtttcca gtgaattgca gttatggaaa caggttacgc
    30661 catagaaatt tacccactga ctgccgtcct tcatcacatc aatcagggag acaaattttt
    30721 cgttacgcag gcgtgttgca taatcaacaa gccgtctgaa acggttgcgg tgagggacat
    30781 cactcttgat ttcgctgtct tctgtgagct cattttcctg cacatcatcc cgcacagacg
    30841 tgtacgcaat caggtcttca aaggaagacc tgccgtcacg tcttttgtgc ggaataatgg
    30901 cattcatcag cagttatctc agcgtcccat taagtctctt cgcagagcat tgacatattc
    30961 agtaatggca acaagaacct ctgcatactc cttatcacca gtgcgttttc cctccacaaa
    31021 cagatgcttc tgaaggcggc caagacgcat aatctcatca atcatacggt cgtcagtacg
    31081 gcagttaacc accttatcca gtgaagaact tcgtataaat gttgaaaccg tctgtccact
    31141 tgactccgct ttttctctta cgatttcagc ctcatcctcc gtgaatctcg ccgtgaattt
    31201 tacaattctc ttcctgttct cactgcccgt tcgggttttc ttctcactca tggtctgctc
    31261 cttaccgggg tgtcggggca aagccctgac caggtgggga atgtctgagt gcgcgtgcgc
    31321 ggtccgacat tcccacatcc tgtcccgttt ttcaggccat tatagcctgt tatttttgtg
    31381 catcatagtc ttatgcagac ggcagaggca gggcgcagcc ctgccaggag tcagtttttg
    31441 gtgataaaat caggcttcaa tattaaacgg agagaaagtg ccaatggcgt ctaaaaaatt
    31501 ttactcagat gatgacatcc agctggcaaa agctgcttta tcggaattgc cagatctcac
    31561 cgctcaacgg aaaacattgc gtgattttct tgatgccatt cgtgatgaca ttattgtcct
    31621 ggtaaggaca aagggttata cactggcaga tgttcgtaac acgctcaaaa atgcagggta
    31681 tgaagtagga gagaaagcac ttcgtgacat tattcgggaa gcagaatcga aaaaaccatc
    31741 ccgccgttct tcctctaaaa cagcacctaa aaagacagat tcaggcagga aagatggcat
    31801 cgatatgaac aacaattgat gtgacaatgt cacatcgtgt tctgagatta tgatgattac
    31861 agaaaaaaaa gccccggcat aaataccagg gcttttctgt ttaatcagac tcgtggcggc
    31921 aatacacctg gataatgccg tgcaataata tcattcagtt tttcaacaag gtcagaacgc
    31981 ttaaagacaa tatgtacggt ccctttctgg tagccacgca ccgtaaaata ttccccttct
    32041 aacagcttac cgacattatc gccgttcccg agaaattcac catactgcgc cccgatgctg
    32101 ttcctgaagt caggaacgtt gcgcccctcc agcagataaa acggacgtac caggtcatcc
    32161 agtgcctccc gtccgctgca gtccagcgtc acacgcccgt tactccagcg gtacagcagg
    32221 ttttcaataa tgattttctt gcccagacgg cagggattat tggttctgta atcccatgac
    32281 aatttccggt agacatcaat cagtccctgt tcaaacgtct gcatcttgct ggcattcaga
    32341 tggcggaatg ttgccagtac attgtccagg gtgatttcag gacaggtgtc gctcatcagc
    32401 tggctgttcc actcatcacg ctggcatgaa ctcatgaagg tgtacatgcc cgtatctgtc
    32461 atcaggcggt cccagatttt ccggtcggca agacggatga tattcctttt cgcttcttcc
    32521 ggtttatcca tgcatcgaac ggagtcagtc acacagtctt caaacccgta gagaaaaatg
    32581 tcaccggaag catcgagcag aatcctgcgc gctgtcgcca ggcattccat cgcctgcatg
    32641 aacagtgcaa taccttcgtt acgctgcgcg atgatgcggt cgatagccac agacggaata
    32701 acatctgtcg tgccggcagt attcaggtca gcagtgggta aagcctgatt catcacattg
    32761 tctcctcatc gtcacgactg ccgggtcagc caggtcattt cccggaggag cgaagctcct
    32821 tctgccgtcc gggtcttagg gtatttcccc gcagtggacc tttccgacgg gggacgctca
    32881 ccacgtcacg gggcagttgt ccgggggcag atgcaaaagt ttttgacgcc agcggcaaaa
    32941 agttttgtgg ctggtacatc tctcccctgg acgactgaaa cgggatgtgg tcgtgtatgt
    33001 acccccggaa agtccgtctg cggagaacag gccgcagtgc ggcggcagac aggcagagac
    33061 agctgcgcca tcaggcgcag tggccttaca caccgtcagg tgtgcaggga gcgccgcata
    33121 cggcgcgact gaagaaaaac gacggcacaa caatggcatg tggtggtcac tgatgcacca
    33181 ctcccgcaca ggtgaaaaca gagaaaacag cacgacattt gcaactagaa aagtgaaatt
    33241 tcttcacttt ttcactctta tttttcgcgc tgaattggtg attataatga taaaataaaa
    33301 tgaagttttt tcacttttta aaaggtactt tatgaccggc tgggaactgc gaatctggcg
    33361 aaaaagcatg ctctggtcac gcgaaaaagc gtccagagaa tttggcataa cacagagaac
    33421 atggcacgcc tgggaaaatg cggaacaggt ggacgtcacc gtctggagga ccacccaggc
    33481 actttcagtc cgtgacctgc tgcctcacat gcaggggatg cgcaaggcag atatcatcag
    33541 gcgtctggaa aatgaactgg gggaaacggc agaggatgtg tgagcgtgat tcacagcctg
    33601 gctgaatgcc aggctactgc tcatcaggaa acagcttttt cagttcttca ccggaaagtc
    33661 ccgtgacctc ctgaatccgt tccggcggcc agcctatatc ccgcagccgg caggctattt
    33721 cccgcgccac ttcaagaacc ccttgtttaa aaccttgttt aaaaccttct ttaaaaccct
    33781 cgtcgaaacc tttctgtata agacgttctg caatcgtcat gctgtttgct ctgctcctgc
    33841 tgacaaaaaa gcccgggatt ttgccgggct ttgtggttct catcagtgag tgattttctt
    33901 cagttcgtca tcggaaagtc cggtagcctg cttaatctgt tccggcgtca tccccatttt
    33961 ctgaagctgg cacgcaatag ccagggcttt ttcaagcgct ccttccatgc gtccttccat
    34021 gcgtccttcc atgcgtcctt ccatcctgcc ttcctggcga cctttttgct caagacgttc
    34081 tgcgatagtc atcagtttat cctcatgctg cggcagacgc tcagccagcg cccgcataaa
    34141 ttccggtgat acagagtccc ctgcgttaac gatgtaatga atcagaacac gcacctgctc
    34201 gtctgaaatt tccaccatga cctgcgggag tttgtccagc agctccatca tgtcacgatg
    34261 gcggatatgc ttcatcagca gcgtcagtgc ggccatccgg cgatggttca tgatttcatt
    34321 gtcatcaacg acagtgatat caaccagcgg aaacggcttt gtgtatattt tagccgcaag
    34381 tgccggattc tcaaaacagt ccagccagtt catgctgtac gggtacgggc tgcgctcacc
    34441 gtgatagaac agcaccggaa tcaccagtgg caacgttttg tgctgctcca gatgtctctg
    34501 catcgccgcc acactgtagc gtaacatgcg aaaaggcatg tacagatttt cggtgctctg
    34561 gtgctcgatg agcgtataaa catagccgtc ttcacccccg gtagttttca cgctccagag
    34621 gatatcgctg gcgtactgat gcaggtccgg ctctacaaag gtggcgggtt ccagcttcag
    34681 cgtggacagg tcgcacaact gccggtactc cgccggaaga tgcagttcca gaaaatctct
    34741 ggcgacgtcg ggattcgcca ggaacgaccg gaacgcggca tcatgcggcg taggcgtcgt
    34801 ggtgtttttc ttcttactca cggttcctcc atcaaatcag cagcatcgca gcaatcatcg
    34861 cccaaatccc cacaaccgag aggacagcaa ttgcgttcaa agccatgaca acaatagccg
    34921 cgttctgagt cacggcgtat ttcttcagca atctcttctg cggaaaggta atcaaactcc
    34981 tcacgggcca gttccaggac cagtagatgt tgttctgtcg gcaatgtggc cacaaaatta
    35041 tcaatagcag cactcaaacg tcgctgatat agctcataaa caagaccaca acggggcatt
    35101 agttcttcgg ataatagttc cagttttgag cgaattctgt cagcgttgaa agtgacttca
    35161 tcaaaagaca tatgaaaagc tttgtcagta tcaaaacaag acttcattgt gaaccgcccc
    35221 ggggatcctg gagactaaac tccctgagaa agaggtaaac aggatgacta aaaatactcg
    35281 tttttccccc gaagtccgtc a
//
LOCUS       DAJVUJ010000022        35013 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.022, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000022 DAJVUJ010000000
VERSION     DAJVUJ010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35013
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.022"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            32..1270
                     /gene="dsdA"
                     /locus_tag="OQQ48_001023"
                     /pseudo
     CDS             32..1270
                     /gene="dsdA"
                     /locus_tag="OQQ48_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
     gene            complement(1378..2916)
                     /gene="emrY"
                     /locus_tag="OQQ48_001024"
     CDS             complement(1378..2916)
                     /gene="emrY"
                     /locus_tag="OQQ48_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HCS1951871.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(2916..4079)
                     /gene="emrK"
                     /locus_tag="OQQ48_001025"
     CDS             complement(2916..4079)
                     /gene="emrK"
                     /locus_tag="OQQ48_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HCS1951872.1"
                     /translation="MEQINSNKKHSNRRKYFSLLAVVLFIAFSGAYVYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIKSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKNEDIAEMPELASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            4495..5109
                     /gene="evgA"
                     /locus_tag="OQQ48_001026"
     CDS             4495..5109
                     /gene="evgA"
                     /locus_tag="OQQ48_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HCS1951873.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            5114..8707
                     /gene="evgS"
                     /locus_tag="OQQ48_001027"
     CDS             5114..8707
                     /gene="evgS"
                     /locus_tag="OQQ48_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HCS1951874.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALEEGEVDIVLSHLVASPPLNDDIAATKPLIITFPALVTTLHDSMRPLT
                     SSKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVVLNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDIIPGAIYSEDRENNVSFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIKVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDHAVYI
                     CGWQDITETRDLIHALEVERNKAINATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLV
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEHPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILITFQHETHKDLPAAFQALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(8763..9908)
                     /gene="yfdE"
                     /locus_tag="OQQ48_001028"
     CDS             complement(8763..9908)
                     /gene="yfdE"
                     /locus_tag="OQQ48_001028"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HCS1951875.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDYDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWETLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFNTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLARIHEVGVPVAPLLSVAEAIKLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(9982..10926)
                     /gene="yfdV"
                     /locus_tag="OQQ48_001029"
     CDS             complement(9982..10926)
                     /gene="yfdV"
                     /locus_tag="OQQ48_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HCS1951876.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIVYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(10996..12690)
                     /gene="oxc"
                     /locus_tag="OQQ48_001030"
     CDS             complement(10996..12690)
                     /gene="oxc"
                     /locus_tag="OQQ48_001030"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HCS1951877.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVIGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSSVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(12744..13994)
                     /gene="frc"
                     /locus_tag="OQQ48_001031"
     CDS             complement(12744..13994)
                     /gene="frc"
                     /locus_tag="OQQ48_001031"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HCS1951878.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(14507..15139)
                     /locus_tag="OQQ48_001032"
     CDS             complement(14507..15139)
                     /locus_tag="OQQ48_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HCS1951879.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVQQTQKTAA
                     AERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKT
                     NLNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVME
                     NQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            15435..15710
                     /locus_tag="OQQ48_001033"
     CDS             15435..15710
                     /locus_tag="OQQ48_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1951880.1"
                     /translation="MKVNLILFSLFLLVYIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDAPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(15787..16029)
                     /locus_tag="OQQ48_001034"
     CDS             complement(15787..16029)
                     /locus_tag="OQQ48_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HCS1951881.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTEIFMFLAPACGLMIRFMVGYGRR"
     gene            16382..17302
                     /gene="lpxP"
                     /locus_tag="OQQ48_001035"
     CDS             16382..17302
                     /gene="lpxP"
                     /locus_tag="OQQ48_001035"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HCS1951882.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHCRFKTRPVGESSLYI"
     gene            17658..17729
                     /gene="ypdK"
                     /locus_tag="OQQ48_001036"
     CDS             17658..17729
                     /gene="ypdK"
                     /locus_tag="OQQ48_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HCS1951883.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(17794..19032)
                     /gene="alaC"
                     /locus_tag="OQQ48_001037"
     CDS             complement(17794..19032)
                     /gene="alaC"
                     /locus_tag="OQQ48_001037"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HCS1951884.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRQDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWIVEMPKASMYVWAKI
                     PEPYAAMGSLVFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            19409..21106
                     /gene="ypdA"
                     /locus_tag="OQQ48_001038"
     CDS             19409..21106
                     /gene="ypdA"
                     /locus_tag="OQQ48_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HCS1951885.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            21121..21855
                     /gene="ypdB"
                     /locus_tag="OQQ48_001039"
     CDS             21121..21855
                     /gene="ypdB"
                     /locus_tag="OQQ48_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HCS1951886.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            21868..22725
                     /locus_tag="OQQ48_001040"
     CDS             21868..22725
                     /locus_tag="OQQ48_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS1951887.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNSQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYTQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(22728..25223)
                     /gene="ptsP"
                     /locus_tag="OQQ48_001041"
     CDS             complement(22728..25223)
                     /gene="ptsP"
                     /locus_tag="OQQ48_001041"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCS1951888.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQAQRVLEEYIQVRFIDSDSIQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASSVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRESDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPIDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(25248..26285)
                     /gene="ypdE"
                     /locus_tag="OQQ48_001042"
     CDS             complement(25248..26285)
                     /gene="ypdE"
                     /locus_tag="OQQ48_001042"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCS1951889.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGTRSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHSAELPAEVWLVASSSEEVGVRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQICNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMQQLLSALIKRLT
                     RETVVQLTDFR"
     gene            complement(26285..27370)
                     /gene="ypdF"
                     /locus_tag="OQQ48_001043"
     CDS             complement(26285..27370)
                     /gene="ypdF"
                     /locus_tag="OQQ48_001043"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCS1951890.1"
                     /translation="MTLLASLRDWLKEQQLDAVLLSSRQNKQPHLGISTGSGYVVISR
                     ESAHILVDSSYFVEVEARAQGYQLHLLDATNTLTTIVNQIIADEQLQTLGFEGQQVSW
                     ETAHRWKSELNAKLVSATPDVLRQIKTPEEVEIIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKASFDTIVASGWRGVLPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFDVYQIVLQAQLAAISAIRPGVRCQQIDDAARRV
                     ITEAGFGDYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            complement(27385..28632)
                     /locus_tag="OQQ48_001044"
     CDS             complement(27385..28632)
                     /locus_tag="OQQ48_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCS1951891.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGVIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(28654..28980)
                     /locus_tag="OQQ48_001045"
     CDS             complement(28654..28980)
                     /locus_tag="OQQ48_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HCS1951892.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(29199..30164)
                     /gene="glk"
                     /locus_tag="OQQ48_001046"
     CDS             complement(29199..30164)
                     /gene="glk"
                     /locus_tag="OQQ48_001046"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HCS1951893.1"
                     /translation="MIKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            30368..31624
                     /locus_tag="OQQ48_001047"
     CDS             30368..31624
                     /locus_tag="OQQ48_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HCS1951894.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTINIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            31739..32065
                     /locus_tag="OQQ48_001048"
     CDS             31739..32065
                     /locus_tag="OQQ48_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCS1951895.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(32206..33444)
                     /locus_tag="OQQ48_001049"
     CDS             complement(32206..33444)
                     /locus_tag="OQQ48_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCS1951896.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            33780..34982
                     /gene="nupC"
                     /locus_tag="OQQ48_001050"
     CDS             33780..34982
                     /gene="nupC"
                     /locus_tag="OQQ48_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HCS1951897.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
BASE COUNT         9234 a         8692 c         8083 g         9004 t
ORIGIN      
        1 gtcacggcga actgcctttc aaggttgaag tggcacgacc tcattggctg aaggtttacc
       61 ttatgttggc ctgaccgaac aggatgttca ggacgcccat gcgcgcttat cccgttttgc
      121 accctatctg gcaaaagcat ttgctgaaac tgctgccact ggggggatta ttgaatcaga
      181 actggttgcc attccagcta tgcaaaaacg gctggaaaaa gaatatcagc aaccgatcag
      241 cgggcaactg ttactgaaaa aagatagcca tttgcccatt tccagctcca taaaagcacg
      301 cggcgggatt tatgaagtcc tggcacacgc agaaaaactg gctctggaag cggggttgct
      361 gacgcttgat gatgactaca gcaaactgct ttctccggag tttaaacagt tctttagcca
      421 atacagcatt gctgtgggct caaccggaaa tctggggtta tcaatcggca ttatgagcgc
      481 ccgcattggc tttaaggtga cagttcatat gtctgctgat gcccgggcat ggaaaaaagc
      541 gaaactgcgc agccatggcg ttacggtcgt ggaatatgag caagattatg gtgttgccgt
      601 cgaggaagga cgtaaagcag cgcagtctga cccgaactgt ttctttattg atgacgaaaa
      661 ttcccgcacg ttgttccttg ggtattccgt cgctggccag cgtcttaaag cgcaatttgc
      721 ccagcaaggc cgtatcgtcg atgctgataa ccctctgttt gtctatctgc cgtgtggtgt
      781 tggcggtggt cctggtggcg tcgcattcgg gcttaaactg gcgtttggcg atcatgttca
      841 ctgctttttt gccgaaccaa cgcactcccc ttgtatgttg ttaggcgtcc atacaggatt
      901 acacgatcag atttctgttc aggatattgg tatcgacaac cttaccgcag cggatggcct
      961 tgcagttggt cgcgcatcag gctttgtcgg gcgggcaatg gtgcgtctgc tggatggctt
     1021 ctataccctt agcgatcaaa ccatgtatga catgcttggc tggctggcgc aggaagaagg
     1081 tattcgtctt gaaccttcgg cactggcggg tatggccgga cctcagcgcg tgtgtgcatc
     1141 agtaagttac caacagatgc acggtttcag cgcagaacaa ctgcgtaata ccactcatct
     1201 ggtgtgggcg acgggaggtg gaatggtgcc ggaagaagag atgaatcaat atctggcaaa
     1261 aggccgttaa taacgtttca acgcagcatc gcaatccttt ccctgggtga gcgatgctgc
     1321 cgatggcgca gacttaagat ccccggtctt acccgctata accccctcct tttatcatca
     1381 cccaacgcct ttcgctgtaa acggcggttt cgcaaaccaa accaaaaccg taagtaagat
     1441 aaaagcaata gccgccatac ggaaaatctc atttgcagaa atagaaagtg actgctgggt
     1501 tatttcatta ttaatttcat taagaactcc tgaaagcgaa ccataatatt tatccataat
     1561 ttgtgacgat gaattaaaca cggggttaaa ttgatcgatg gttgctgtca actgactatg
     1621 gtgtaacgat tcgcgtcgtc cccacagcgt cattgtcaac gacgtaccaa ctgatcctga
     1681 caaggtacga aaaaaattac tcatactcga ggcattggca aatttattat ctggcaagcc
     1741 tgaaaacgaa atcgttgtta agggtaaaaa gaaacaggca acggcgaatc cctgaaaaaa
     1801 ctgcggcaaa atgatgcctg taaaatcaat cgttggcata aatgtcacag aacgccagta
     1861 atagcaaacc gcatacatca aaaaactaaa tgtcactaac aaccgcatgt ctattttgtt
     1921 gccataacgt cctatcaaag gtgaaattaa tagtggcatg atgccgatgg gcgcataagc
     1981 aagtccggcc catatcgcat tataccccat cgtttcctgg agtaactgcg gcataaggac
     2041 gatcgctcca gagtaaaata aatacgcgca tgtgatactc acaataccaa tggtgaagtt
     2101 acgggactta aacaaactga gatcaagaat cgggttctct gaggtcgact cccaaatgac
     2161 taaagagatc agagaaataa ctgatactac tgttaatatt attattgtac tcgagttgaa
     2221 ccaatccaga tcgcgccctt tatcaagcat aatttgcaag ccaccaacac cgagcactaa
     2281 cagggtcagt cctggtagat tcattttgac cggtgaagtc tcagtttctc ttcctttaag
     2341 taaggttaag cataatgtca ggacgataat ccccataggg acattgatta aaaatatcca
     2401 accccagcta aagttatcac aaatataacc gcccaatatc ggcccacata tcggagcgat
     2461 aatcacggtc attgaccata atgccagagc aaatgttctt ttttctggcg gataattcct
     2521 taataataaa ctctgtgaca gtggaattaa cggccccgcc attaacccct gaacgactct
     2581 aaaaaatatc agcacatcaa gattggtcga taggctacac attaatgaag acagcgaaaa
     2641 aaaggttact gaaagtaaaa ataatcttaa ttcgcctatt ctttgtgcca acctgccagt
     2701 aacagggatc gcaatggcat ttgctacacc aaacgaggtg ataacccagg tgccttcgtc
     2761 tgttgatgct cccagaaagc cagatattgt cggtattgcg acgttagaaa tagtggaatc
     2821 caacatttgc ataaatgtcg ctaatgacaa tgcaatagtg acgcaccata acgtcccacc
     2881 ggttaatggt gccggagttg atttagtgat tgccattaaa gttgtccatt atgcgaaata
     2941 atgttgctaa tttctttttc tatcggactg gtatcgataa ctaaagcctt actggtataa
     3001 gccggcatgg aggtcacggt tgaagccagc tcaggcatct cggcaatgtc ttcgttcttc
     3061 gtatcaatag ttgctgtcat cgataaacca atacgcaagg gatgttccat gagttctttt
     3121 ggatcaagag aaacttcaac cggtacacgc tgaacgattt tgatccagtt ccctgtcgca
     3181 ttttgtgcag gtaataagga gaacgcattg ccggttccca tattgatccc tgtcacccga
     3241 ccatgaaaca caacattttc accataaaga tcgctgataa tattgaccga ttgaccaatc
     3301 cgcacatccg tgagttgtgt ttctttaaag ttggcattaa cccacatttg acgtgccggt
     3361 acgacagcca ttaacgattg tccggggctc actgtttcgc cgacctgaac acttctctgg
     3421 gcaatatagc cggtaaccgg actcttaata tccgtacgtt taagcgccaa ccaggcttct
     3481 ttagttgcat ccgccgcttc aacgacttgt ggctgacggt ttaatggtgt gttcattact
     3541 aaagctttat tcgctttata agcctggata gcggcattca atgccgcttt gctacttatt
     3601 aacgtatctt tggtatgctc cagcgtttct tttgaaataa ccccctgctt cgctaacggc
     3661 actcgacggt tataatcttc taaagattgt tgatactgaa tacgtgctga agcgacttcg
     3721 gcactgtatt gtttatcctg taagtatagt ttattcgttt gccgaacaat atttgccaga
     3781 ttatttttag ctttattgag tgcgatagtg gcatcagttt tatccagtga aactaaaatg
     3841 tcaccttgtc gaacgtagtt cgtatcttta tgattaacga cagtgacact acctgagact
     3901 tgtgcagaaa ttggatctgc attccccgtg acataggcgt catctgtact aatcatgtct
     3961 tctaattcca ttgaccaata gacataggca cctgaaaacg caataaataa aactaccgcc
     4021 aataaagaaa agtattttct tctgttagaa tgttttttat ttgaattaat ctgttccact
     4081 attatctctc atttctcata gatgaaattt atgaattgaa tgaaaagagc attcattctt
     4141 catatcaaac acagtgcatg tgttgcacat tatcccagga acgttggagg cctaatttaa
     4201 atgaggtgca aaaaaaacaa cagaggacta aaccgtggct tttgcaatac aattcttacg
     4261 cctgtaggat tagtaagaag acttatagtg ctaacttgaa actataaatc atcggtacaa
     4321 cccctgattt tattgttgac atttcattta tgccgactat ttatatggta tacttgtcga
     4381 attatcttaa aggaagctca gattttctta tttttattga gaaaatgaga tgacgcctta
     4441 tgtctgtatt actacaggga gaagggagat gcttcattgc aaagggaata atctatgaac
     4501 gcaataatta ttgatgacca tcctcttgct atcgcagcaa ttcgtaattt attgatcaaa
     4561 aacgatattg aaatcttagc ggagttgact gaagggggaa gcgccgttca gcgggtggaa
     4621 acacttaagc ctgatatcgt catcattgat gtcgatatcc ccggagttaa cggtatccag
     4681 gtgttagaaa cgctgaggaa gcgccaatat agcggaatta ttattattgt ctccgctaaa
     4741 aatgaccatt tttacgggaa acattgtgct gatgctggcg ctaatggatt cgtgagtaaa
     4801 aaagaaggca tgaacaatat cattgcggct attgaagctg caaaaaatgg ctactgctat
     4861 ttccccttct ctctcaaccg gtttgttgga agtttaacgt ccgaccagca aaaactcgac
     4921 tccttatcga aacaagaaat tagtgtcatg cggtatattc ttgatggcaa ggataataat
     4981 gacattgctg aaaaaatgtt catcagcaac aaaactgtca gcacttataa aagtcgcctg
     5041 atggaaaaat tagaatgtaa atcactgatg gatctttaca cattcgcgca acgtaacaaa
     5101 atcggctaat cacatgaagt ttttacccta tttttttctt ctctgttgtg gtctttggtc
     5161 gaccataagt ttcgcagacg aagattacat cgaatatcgt ggcatcagta gtaacaaccg
     5221 tgtcacactt gatccagtac gtctgagcaa caaggaatta cgttggttag cgagcaaaaa
     5281 aaatcttgtg attgcagtac acaagtccca aacggctacc ttgttgcata ccgattcgca
     5341 gcaacgggtt cgtggtatta atgctgatta tttaaatctt ttaaaaagag cgttaaatat
     5401 caaattaaca ctccgggaat acgccgatca tcaaaaagca atggacgcgc tggaagaagg
     5461 tgaagtcgat atagtgttat cacatttagt tgcttcgccg cctcttaatg atgacattgc
     5521 tgcaaccaaa ccactgataa ttacctttcc ggcgctcgtc accacccttc acgattcaat
     5581 gcgaccgctt acctcatcaa aaccagtaaa tattgctcga gtagcaaatt atccccctga
     5641 cgaggtaatt catcaatcat ttccaaaagc aacaattatc tcttttacaa atttatatca
     5701 ggcattagca tccgtctcag ccggacagaa tgattacttt attggtagta acatcattac
     5761 cagcagtatg atttcccgct atttcactca ctccttaaat gtagtgaaat attataactc
     5821 accgcgtcaa tataattttt tcttgaccag aaaagaatct gtcgttctta atgaagtact
     5881 caatcgattt gttgatgctt taacaaatga agttcgctat gaagtttcac aaaattggct
     5941 tgatacagga aacctggcct ttctgaacaa accattagaa ctcactgaac atgaaaaaca
     6001 gtggattaag caacatcccg atttaaaggt gctggaaaat ccttactctc ctccctattc
     6061 tatgacggat gaaaatggct cggttcgggg cgttatgggg gatattctta atattattac
     6121 cttgcaaaca ggtttaaatt tttctccgat caccgtttca cacaatatcc atgccggaac
     6181 acagcttaac cccggtggat gggatataat acctggcgct atttatagtg aagatcgaga
     6241 aaataatgtt tcatttgcag aagctttcat aacaacgcca tacgtttttg tcatgcaaaa
     6301 agcacctgac agtgaacaaa cattaaaaaa aggaatgaaa gttgccattc catattatta
     6361 tgagctgcat tcgcaattaa aagagatgta tccggaggtt gagtggataa aagtcgataa
     6421 cgccagcgct gcatttcaca aggtcaagga aggtgaactt gatgctctgg tcgcgacaca
     6481 gctaaattcg cgttacatga tcgatcatta ctatcctaat gaactgtatc attttcttat
     6541 tcccggcgtt ccgaatgcat cgctttcgtt cgcttttcct cgcggagaac cggaacttaa
     6601 ggatattatt aataaagcac tgaatgcaat tcccccaagc gaagttctgc gcctgacgga
     6661 aaaatggatt aaaatgccca atgtgaccat tgacacatgg gacctctata gcgagcaatt
     6721 ttatattgtt acgacattat ccgttttatt agttggcagt agccttttat ggggattcta
     6781 cctgttacgc tcagttcgtc gtcgtaaagt cattcagggt gatttagaaa accaaatatc
     6841 attccgaaaa gcactctcgg attccttacc aaatccaact tatgttgtaa actggcaagg
     6901 taatgtcatt agtcataata gtgcttttga acattatttc actgcggatt actacaaaaa
     6961 tgcaatgtta ccattagaaa acagtgactc accctttaaa gatgtttttt ctaatgcgca
     7021 tgaagtcaca gcagaaacga aagaaaatcg aacaatatac acacaggtat ttgaaattga
     7081 taatggcatc gagaaaagat gcattaatca ctggcataca ttatgcaatc tgccagccag
     7141 cgatcatgct gtttatattt gtggttggca agatattacc gagacgcgtg atttaattca
     7201 tgcactcgaa gtagagagaa ataaagcgat caatgcaact gtcgcaaaaa gtcagtttct
     7261 ggcaacaatg agtcacgaaa taagaacacc aataagctcc attatgggct tcctggaact
     7321 tctgtcgggt tctggtctta gcaaggagca acgggtggag gcgatttcac ttgcctacgc
     7381 caccggacaa tcactcctcg gcttaattgg tgaaatcctt gatgtcgaca aaattgaatc
     7441 gggtaactat caacttcaac cacaatgggt cgatatccct actttagtcc agaacacttg
     7501 tcactctttc ggtgcgattg ctgcaagcaa atcgatcgca ttaagttgca gcagtacatt
     7561 tcctgatcat tacctggtta agatcgaccc tcaggcgttt aagcaggtct tatcaaattt
     7621 actgagcaat gctctcaaat ttaccaccga gggggcagta aaaattacga cctccctggt
     7681 tcacattgat gacaaccacg ctgttatcaa aatgacgatt atggattctg gaagtggatt
     7741 atcgcaggaa gaacaacaac aactctttaa acgctacagc caaacaagtg caggtcgcca
     7801 gcaaacagga tctggcttag gcttaatgat ctgcaaagaa ttaattaaaa atatgcaggg
     7861 cgatttgtca ttagaaagtc atccaggcat aggaacaaca tttacgatca caatcccggt
     7921 agaaattagc cagcaagtgg cgactgtcga ggcaaaagca gaacacccca tcacactacc
     7981 tgaaaagttg agcatattaa tcgcggatga tcatccgacc aacaggctat tactcaaacg
     8041 ccagctaaat ctattaggat atgatgttga tgaagccact gatggtgtgc aagcgctaca
     8101 caaagtcagt atgcaacatt atgatctgct tattactgac gttaatatgc cgaatatgga
     8161 tggttttgag ttgactcgca aactccgtga gcaaaattct tccttaccca tctgggggct
     8221 tacagccaac gcacaggcta acgaacgtga aaaagggtta agttgcggca tgaacttatg
     8281 tttgttcaaa ccgttgaccc tggatgtact gaaaacacat ttaagtcagt tacaccaggt
     8341 tgcgcatatt gcacctcagt atcgccacct tgatatcgaa gccctgaaaa ataatacggc
     8401 gaacgatcta caactgatgc aggagattct catcactttc cagcatgaaa cgcataaaga
     8461 tctacccgct gcgtttcaag cactagaagc tggcgataac agaactttcc atcagtgtat
     8521 tcatcgcatc cacggtgcgg ctaacatcct gaatttgcaa aagttgatta atattagcca
     8581 tcagttagaa ataacacctg tttcagatga cagtaagcct gaaattcttc agttgctgaa
     8641 ctctgtaaaa gaacacattg cagagctgga ccaggagatt gctgttttct gtcagaaaaa
     8701 tgactaaata gcggctccca caatgttcaa atgtgggagc tatttaccag cacatcttac
     8761 atttatgatg agaactcctg gcgaatttgt tccccatgct ggtcgagcgt tgccgctccc
     8821 ggcataacat gcgggtccgc gcagccgctg atttttatcg gattacccgg catcattatt
     8881 cccccggctt caatcaacat atttctcgcc tgagtttgtg gcaatttaat ggcctcagcc
     8941 acacttaata acggcgcgac gggtacacca acttcatgta ttctggctaa ccaaacttca
     9001 gctgcctgcg tttttaacgt ccgctcaata tattgtttaa gaatagcctg gttttgtacg
     9061 cgtaaaatat tgctgctaaa tcggggatca ttaaccagtt ccgtaagctc cagtgcctgg
     9121 cataacgcag aaaaaagctt gtcattacca caacaaatcg taatcggctt atcctgagta
     9181 ttgaaaacat caaaaggtgc catgtaggga tggcgatttc ccagacgttg tggtgacttc
     9241 cctgtcgcga tatatgccat cagaccatgc tccagaaaac tcagcgtggc atcaaacatc
     9301 gctatatcga catgcgcccc tctctggctc ttttcgcggc cataaagtgc actcactatt
     9361 ccgctgaata aatagacacc gccgcataga tccgcaagag atgtaccaac gcgcactggc
     9421 ggagcatcag gatatcctgt ttccatcatt atcccgctca ttgcctgaat gatggtatcg
     9481 taggcaggag catcttttag cggaccggta tgtccgaaac ctgacgatga agcatatatg
     9541 aggcgcgggt tgatttcttg aagcgtttcc catgaaaacc ccagtttttc cattgtacct
     9601 gggcgaaaat tctcagctaa tacatcagct tgtttgagca tatttataaa tatactttta
     9661 tcgtaatcat tctttaaatc aagaaccaca ctctctttgc catgattaat aaaactgtaa
     9721 tagagtgact gtccatccac atagggacca aatgtgcggg tatcatcacc atgacccggc
     9781 ggctcaactt taattaccct tgcgcccata ttacaaagaa gttgagttcc gaaaggtcca
     9841 ttaaggacat gtgtcatatc gataactaat aagccttcaa acggcccttt gctttcatta
     9901 tttgtcattt gcttatgcct tgaaaaaagc cgaaggtgaa aagacacttc ggcatttatg
     9961 taaaatcaat aacatgatta attatgaaac cagtcgactg acataaatcc acaagggagc
    10021 cgtgacgaca aagcccagta cgctcacagc caatgacgct gtaccagtac gggtgtagac
    10081 attaaaccgg ctggcaataa taattccgga gaatgccggc ggtaatgcgc ctgccagtac
    10141 catcatctgc agatgttcgc tgttcaaatg acatgccata ccaacgagaa gcagtgccag
    10201 tggcatcaga atcagcttca ggaaggtgtt ataaacaatt tcggcactga actcgaattt
    10261 atgtgcagcc agagtcaacc ccgcagcgaa taccgctacc cctgagttag ccttcgcaat
    10321 cagattaaag gttgggtccc atgctgccgg aatttttacc ccaaccaaca ccaggatcgt
    10381 tgccagaaca ggtgcccata ctactggctc ctttgccgca gaaattaatg cactcagatt
    10441 actattcttc ttaccatccg ctcctgaaga aggattcagc aaatacagac caataggaat
    10501 agtaattgcg ttaacaataa tagaaataat tgctaccact aaacctgttg atacggaatc
    10561 accataaata ggatcgagaa ctgcaaaccc caggaatcca atggtaggtg aacctgcaat
    10621 taatgcacat acagctgctt ctgcatgggt acgtttaaaa aatttgtagc aaccgaacca
    10681 ggagaaaaag aaacatccga caataaccac aagtgataca agggtcagac gagtgtccgc
    10741 aaaaatcatt tccctgtttg cccgagtaat agatacaaat agagccgcag gaagcgcgta
    10801 gtttaatacc agtttattaa atgcgcgggc ttgatcttct gaaaaagttt ctcgtctgcc
    10861 gctaaaataa cccaacagca tgattacgat aataggcaat aaatcgccaa taaaaaatgt
    10921 tagcataata gtatttccga taagggtccc ctcaccatcg cataatgaat tggcaagagg
    10981 agacgatgtc agtatttaat taccagcgac ttgttttggg ttaagtttgg taatatggcc
    11041 actttcagtt cctgctgcag ggtcgatgac cacattaata atggtcggtt tgcgcgactg
    11101 aataccggtg gttaaagcat gacgaagttc atctgtcgtg gtgacgttat agccaacgcc
    11161 acgaaacgca tccattaatt tgtcatacct tgcatggtgc aacagatccg ttggagatgg
    11221 tgcaccagcg ccactgagat caacaccgtc tcctctgtag atgccgccat tattaaaaat
    11281 aacgatcgtc accggcaggt tatatcgaca aatcgtttca atttccatcc cactgaaacc
    11341 aaaagcacta tcaccttcaa tggcgacaac cggagaacca gaagtcacgc tagcaccgat
    11401 ggcatagccc ataccgatgc ccatgacacc ccaggtacca caatccagac gacgacgtgg
    11461 tttatacata tcaataatat ttcgtgcatt atccagggta tttgcacctt cattaactaa
    11521 ataaatatcc tggttctcgc gcaatacgtc gcgcacagaa cttaatgcat taaagtaatt
    11581 taatggttga gtatctgtac ttaatttttc atgcattttt tgtgcatttt gctgcttgtg
    11641 gatatttaaa atatcgcgcc ataccagtgg agtcgtaaat gtgttttgtt tcagttctgc
    11701 cagcatacct tgcatactgg atgcaatatc accaatgact ggcacagcaa tggggcggtt
    11761 gctgtcaatt tcctgcggtt caatatccag ttgaataaac tgtgtatctg ccgcccatcc
    11821 ttttttaccg tgtgccaata accaattcag tcgtgcacca acaagcatga caacgtcagc
    11881 atttgccagg gcaaacgaac gcgcagctgc cgcagaaagt ggatgcgtat cttcaaggat
    11941 ccctttcgcc atagacattg gcaggaatgg aatctgagca ctttcaataa attcacgaag
    12001 ctgttcatca gcttgtgaat acgccgcgcc tttgccaagg ataattaatg gccgttcagc
    12061 ttttgctaaa agcgaaattg cgctagtgac tgacttcggg catggcaata atgctggcga
    12121 cggattttca actttaacaa tcgtggttaa cgcttcgtct ttttccatcg tcgcggccag
    12181 gacatttgct ggcaaatcaa gataaactcc gccagggcga cccgatacag agacccggat
    12241 agcgcgtgcc aatgcaatgc caagatcctg cggctgatta acgcgaaatg ctgctttggc
    12301 atacggtttt gccgcattca tttggtccag ctcttcataa tcaccttgct gtaggtcgac
    12361 gatcgcgcgg tcgctggagc cgctaatcat gatcatcgga aaaccattta ccgttgcgtt
    12421 ggccaatgcc gtcaaaccat tgaggaatcc tggcgcagaa actgtcaggc agatccccgg
    12481 tttttgggta agaaaaccgc ttgccgcagc ggcatagcct gccgactgct catgacgaaa
    12541 accaatataa cgaatgcctt ccgcctgggc atggcgtgcc atatccgtca caggaatacc
    12601 tacaacacca taaatagtgt caatattatt ctgttttaat gcttcaacga tgatatgcat
    12661 accatctgtc atttgaagtt gatctgacat gattaataac ctcgaaattt ttataaattg
    12721 ggagccagaa gcccccgtta atatcagatg gcgtggtttt gcttcattgc agcaatttca
    12781 tcgtcgctat aacctagctc ctgcaataca gcagcggtat gttcacctaa tagcggcgca
    12841 gctttaatat ccggcgtaaa agcagagaat ttcattggac aaccaacggt cagatatttt
    12901 ccacgcaacg gttgttccac ttcaacaaca ctgccacttt ggcgcaaaga gggatcaagt
    12961 gaaatttctt tcatacttaa aaccggtgca caaggaatat caaactgagt caaataggcc
    13021 accgcttcat gtttatcaat agtgacagtg tatttttcga tttcagcaaa aatatcgaaa
    13081 atatgtggct gtcgggcatg ggctgtactg tatgccggat cggtaatcca ttctggtttg
    13141 ccgatggctt tacaggtgtt ttcccagttt tgctcctgaa tagtgaaata aatataggcg
    13201 ttcggatcgg tttcccagcc tttacatttc aggatccagc caggctgacc gccaccacct
    13261 gcattaccac cgcggggaac tgcatcacca aatgtaccat tcggatactg cgggtattct
    13321 tccagataac ccaatttatc gagacgctgc tggtcacgta atttcacgcg gcaaaggttc
    13381 aatacggcat cctgcattga catggtgact cgttgcccac gccccgtttt ttcgcgatgc
    13441 agcaaagcag caagcaaacc gatcagcaaa tgcattccgg tgttgctgtc acccaacgct
    13501 gcagcgctta ccagcggcgg accatcccaa aaacccgtag tggatgccgc gccacccgct
    13561 gcctgagcaa cgttttcata ggcttttaca ttcacataag gcgaacactc atcaaaccct
    13621 ttgatcgaac caaaaatcag acgtggattg atttcttgaa tatgctccca ggtgaagccc
    13681 atgtgatcaa tggcccctgg atgaaagttc tcgactaaga tatcagcttc gcggatcagc
    13741 ttttccatta cctctttgcc ttccgctgtt ttggtattta actcaataga acgtttgtta
    13801 ctgttaagca tggtgaagta aagcgcatcg atatcaggaa tatctcgcag ctggtgacgc
    13861 gttacgtcac caacgccggg acgttcaatt ttaataacgt cagcgccaaa ccaggccagc
    13921 atttgagtac aagatgggcc agattgcaca ccggtgaaat cgagaacttt aattccttga
    13981 agtggagttg acatagcaat acctattagt taattaaata tgtgttaatt tagcgtttat
    14041 ttaaggcgta atatcttcta taaataaccc gcaaaatatc gtcgtagctg cactcgatat
    14101 ttttaaaatc ctgtatcaag tgctgaaaat catttcatcg ccacttccaa ttcctttctg
    14161 catggtaagt agtgaggacg aaaagaagaa tatgataaat attgattcgt ggtaatcaga
    14221 gagagtcgcg acatttgtga tgttattctt acaactgtaa gaagcgccgg tgatttacaa
    14281 gtaagggtat gattacgctt tttaaagcgt accataaaac ataacataat aaatgatttg
    14341 tgatgacacg ggattattgg ggtttacttt gaggaagata aaatgcgtgt ttatagaacc
    14401 gcgagcgcca aataatttta tgaccacaac ctgtcgtatt gtggtcataa aaaattgtcg
    14461 gctggaggga gttaaatcct ttacctcaat atactgcctg tcagcattag ttcacaaaca
    14521 gtgcttcgct gtcgacgatg attccatctt ccgcaccttt cagcgccagg tttgcctcgt
    14581 agtattgctt tttatcaagc agtttctgcg catcagcaag tgcattacga gtctgtttaa
    14641 gcggcatcag atattggttt tccattacgc caacaccggc caggcgaagt tcttccattg
    14701 cgcccttttt atcccctttc gccatttttt cattggcaat ttttatcgcg gcttcttttt
    14761 cgggagttgc tacataactc tcagaaattc cgacagatgc attaatgaca atatattggt
    14821 catcattgag attggtcttt ttacctggct tagcgaactt tgcccattcg gtactgtcat
    14881 ctgacagcaa agcggaagct tcattggtca gttcttttgc cttttctgga tcgccatgaa
    14941 acagagccag gcgcgctacc tgcacgtcac gcatcgcata taaaccttgt tcagagatac
    15001 gctctgctgc tgccgttttc tgggtttgct gaacttgctg agtttgctgt tgtgccgcta
    15061 ccggtgcatt atcagcagcc catacagttg aagatgccag aattgctgtg accatcgtag
    15121 ccataattaa acgtttcata cttacctcat tctttaacag ttttagaaaa cgccgccatc
    15181 atattgggtg agcatgaaat gagaaatcag agagcaacgg tcattttgaa agggttatct
    15241 tacagttgta gttattatcg caagaagtat tttagtctta caattgtagg aatctgcttc
    15301 ctgtcgcaga gaactattct tatgtaccac aaagagatgt catttattat atcgatgtaa
    15361 atattgatta attatatgta aatacagcca taacatcgta ggttctcagt gaaccatttt
    15421 caggataact ttctatgaaa gtaaacttaa tacttttcag cttattttta ttggtctata
    15481 ttatggcatg caatgttttt gcattttcca tttctggtgg tgtaagtgag aggagctata
    15541 aagagactga aaaaacatca gcgatgacga ccacacactc tacaaaactt cagccatcac
    15601 aggcgatttt gtttaagatg agagaagatg cgccaccatt aaacctcaca gaagaaatca
    15661 cgcctactta tccgacaaag gcgaattatc ttattcatcc tgtgcgatag cgtctgtcac
    15721 atggcaggtg aacaatcatg tctgatgctg atgtgaacac ctgcccaaat gagccatcct
    15781 gacccactat cgcctgccat accccaccat aaagcgaatc atcaagccac aagccggggc
    15841 aagaaacata aatatctccg tcactagttc attaccgcca gttaaccaca tggtgaagta
    15901 ataaatgagt gcagcgagga tcaccatgcc gaagaaagaa gagaccgccg ataccacgtt
    15961 cagtacctga ccgatgtagc cgcttacgca gacaatacac agggcgagaa acatccataa
    16021 ataaatcata gctcaacctc ctgccgcgct aaaaaacctg taacttcagt cactttaata
    16081 gtaagacaat ccttaacctc tggcaatttt tgctaagcat caatgaaaac agcatgttaa
    16141 atgcaagact gttgtgtacg gaaaaatatt tactttgcac gattaataat cagtagctga
    16201 aagcagtcag cgagtgataa agtacagata ccgcattcat gctcctcgtg gttatgtcct
    16261 gactagtctt tacacacttt acaggaacca ttgtcgtaca tgatggccca accaattgag
    16321 tgtaccgctg tcgacatgga atcttctgcg atacaacaat tcgtatctac agaaggtaac
    16381 tatgtttcca caatgcaaat tttcccgcga gtttctacat cctcgctact ggctcacatg
    16441 gtttgggctt ggtgtactct ggctttgggt acagcttcct tatcctgttc tctgctttct
    16501 cggcacgcgt attggcgcaa tggcgcgacc attcctgaaa cgtcgtgaat ctatcgcccg
    16561 taaaaacctg gaactttgtt tcccgcagca ttctgcggaa gaacgcgaga agatgattgc
    16621 cgaaaacttt cgttcactcg gcatggcgct ggtagaaacc ggcatggcat ggttctggcc
    16681 cgacagtcgc gtacgtaaat ggtttgatgt tgaagggttg gataacctta aacgcgcaca
    16741 aatgcaaaat cgcggcgtaa tggttgtcgg cgtccatttt atgtcgctgg aactgggcgg
    16801 ccgcgtgatg ggactgtgcc aaccaatgat ggctacctat cgtccacata ataatcagct
    16861 gatggaatgg gtgcagaccc gtgggcgcat gcgctctaac aaagcgatga tcggcagaaa
    16921 taatctgcgc ggcattgtcg gtgcactgaa gaaaggtgaa gcggtatggt ttgctcccga
    16981 tcaggattat ggtcgtaaag gcagctcctt cgcgccgttc tttgcggtgg aaaatgtcgc
    17041 cacaaccaat ggcacctatg ttctctcccg tctctctggc gcagccatgt tgaccgtaac
    17101 gatggtaaga aaagcggatt acagcggata tcgtttgttc atcaccccag agatggaagg
    17161 ctacccgaca gatgaaaatc aagccgctgc ctatatgaac aagattatcg agaaagagat
    17221 catgcgcgca ccggagcagt acctctggat ccactgtcgc tttaaaacgc gcccggtggg
    17281 agaatcgtcg ttgtacattt aacaaactgc tcttactctt tccctcctca aatcggccat
    17341 aataacctca tggtttttat ggcttttttc ttaaggtttt ttccttcgct gaatatctaa
    17401 ttattaccgt cagaaattaa actgtcgccg atatgagaca cttgtaagtt actaaaagta
    17461 aataagaaaa tgcctcttgt ctcgtttcag ttttaggcgt acattagcgc cgtctcgagc
    17521 agtttagctc agaattctgg ggtgatgccc acggtaaaat acagggcttt taaggtcgct
    17581 ttgcccagtg tcaactcact gtatcaggtg taatgaagtc attcaggcgt aacagcaatt
    17641 acgcggagag atgtaaagtg aaatatttct ttatgggcat ttcttttatg gtcatcgttt
    17701 gggccggtac ttttgccctg atgatctaaa agcaaactgt gcaatgaaaa acaggagcca
    17761 tcaggctcct gtttgcattt ctgacgacgg tgtttattcc gcgttttcgt gaatatgttt
    17821 gctgctggcg ggtagtaaac cgtcggcacg gaacatcgct ttaatccctc tgatggcctg
    17881 acgaatacgg tcgcggtttt caatcagtgc aaagcgaaca tgcgtatcac catagtcgcc
    17941 aaagccaatc cccggcgaga cacagacctt cgcttcgtta agcaatttct tggcaaatac
    18001 cagcgagccc atggcagcat acggttccgg tattttcgcc cagacataca tcgaagcctt
    18061 cggcatttca actatccagc ccgcttcatg cagcccttta accaatacat cacggcggcg
    18121 tttgtactgt tcggcaatgt cgcgcacgca ctgttgatcg ccctccagcg ccgcaatcgc
    18181 cgccacctgc aacggcgtaa aggtgccgta atcgtgatag cttttaatac gagccagggc
    18241 gctgaccagc gttttgttgc caaccataaa gccgatacgc cagcccgcca tgttgtagct
    18301 tttcgacagc gtaaagaact cgactgccac atcgcgtgca cccggtacct gcatgattga
    18361 cggcgctttc cagccatcgt agacgatatc ggcataggcc aggtcgtgga ccaccagcac
    18421 atcgtagcgt ttcgccagcg ccacaacctt ttcgaagaac tccagctcca cgcattgcgc
    18481 ggttggattc gacgggaagc cgaggatcat catcttcggt ttcggataac tttcacgaat
    18541 ggcgcgctcc agttcgttga agaaatcgac accttccacc agcggcactg agcgtacctg
    18601 cgccccggca atcaccgcgc cataaatatg gatcgggtaa cttggattcg gcaccagcac
    18661 cgtgtcacca tgatccagcg tcgccagcat cagatgcgcc aggccctctt tcgaaccaat
    18721 agtgacgatg gcttctgatt ccgggtcgat ttcaacgtcg tagcgatcct gataccagcg
    18781 ggaaatggcg cgacgtaacc gcggaatgcc gcgtgaagtg gagtaaccat gcgtgtcctg
    18841 gcgctgggcc acagtacata atttttcgac gatatgcggc ggagtcgcac cgtccgggtt
    18901 acccatgctg aaatcgataa tatcttcgcc gcgccgacgc gcagccattt tcagttcagc
    18961 ggtgatgtta aaaacgtagg gcgggagacg atcaatgcgc gtaaagcgac gttcagggcg
    19021 agtgtcagcc ataatttcct cagattaacg ttagcgcccg gaccgtccga gcgacgctgc
    19081 cacaatggtg gctgttttga aaatagcctg attaatttct gtctgtccag cgttttagaa
    19141 catttttatc aatggcttta aatgaggaac tacacttcaa aaacaggaaa tttttctacg
    19201 ctaacaagaa aattaccacc aattttatta acattataat ttcaataaat ttaccagcca
    19261 gaagtgcatt atcaagagat acattgctaa tacaccattt atctttcaat ccccttttaa
    19321 atttatggtt tttcctcaaa aacggcctgc tggtcatcag ccttcaggtt acctatcata
    19381 gaggtttaat ccttattcag agtcacccgt gcacgaaata ttcaacatgc tgctggcggt
    19441 cttcgatcgg gcagcgttaa tgcttatctg cctgttcttt ctcatccgta tccgcctgtt
    19501 tcgcgagctg ttgcacaagt cggcacactc cccaaaagag ttgcttgctg ttaccgccat
    19561 tttctcgctg ttcgccctgt tcagcacctg gtccggcgtt cccgtagaag gctcgctggt
    19621 gaacgtacgt attatcgcgg tgatgtccgg cgggattctg tttggcccgt gggtaggcat
    19681 cattaccggc gtgattgcgg gtattcaccg gtatttaatt gatatcggcg gcgtgacggc
    19741 gatcccctgc tttatcacca gcattctggc gggttgtata tcaggctgga taaacctgaa
    19801 aatccccaaa gcacagcgct ggcgcgtcgg tattctcggc ggcatgttgt gtgagacgtt
    19861 gaccatgatt ctggtaattg tctgggcacc aactaccgcg ttggggatcg atatcgtctc
    19921 taaaatcggc attccaatga tcctcggtag cgtctgtatc ggctttattg tgcttctggt
    19981 gcaaagcgtt gagggcgaaa aagaggccag cgccgcgcgg caggccaagc tggcgctgga
    20041 tatcgccaac aaaacgctac cgctgtttcg ccatgtcaat agcgagtcat tacgcaaggt
    20101 ctgcgaaatt atccgcgatg acattcacgc cgatgcggtg gcgattacta ataccgatca
    20161 tgtgctggcc tatgttggcg tgggtgaaca taactatcag aatggcgatg acttcattag
    20221 cccgactacc cgtcaggcga tgaattacgg aaaaatcatc attaaaaaca atgatgaagc
    20281 ccaccgcaca ccagagattc attccatgct ggtgatccca ttgtgggaga aaggcgttgt
    20341 gaccggaacg ctgaaaattt actactgcca cgcgcatcag ataacctcgt cattacagga
    20401 aatggcggtt ggtctgtcgc aaatcatctc cacgcaactg gaggtttcac gcgccgagca
    20461 gctacgtgaa atggcaaata aggcagagct tcgcgccctg caaagcaaaa ttaatcccca
    20521 ttttctgttt aacgctctga acgctatttc atcgtcaatc cgtctgaatc cggataccgc
    20581 tcgccagttg atctttaatc tgtcgcgtta tctgcgctat aacattgaat taaaagacga
    20641 tgagcaaatc gatatcaaaa aagagctgta tcaaattaaa gactatattg ccattgagca
    20701 ggcccgcttt ggtgacaagc tgacggttat ctatgatatt gatgaagagg tgaattgctg
    20761 cattcccagc ctgctgatcc agccgttggt ggagaacgcc attgtccacg gtattcagcc
    20821 ttgcaaaggt aaaggcgttg tcaccatcag cgttgcagag tgcggaaatc gggtacgcat
    20881 tgcggtgcga gataccgggc acggcatcga tccaaaggtg attgagcggg tcgaagcgaa
    20941 tgaaatgccg ggcaataaaa ttggcctgct gaatgtccat catcgcgtga agttattgta
    21001 tggcgagggg ctgcatatcc gccgcctgga gccggggacg gaaattgcgt tttacattcc
    21061 taaccaacgc accccagtcg cctcacaggc tacgttattg ctttgagccg gagtgatatt
    21121 gtgaaagtca tcattgttga agacgaattc ctggcacaac aggaactgag ctggctaatt
    21181 aaagagcaca gccagatgga gattgtcggc acctttgacg acggtctgga cgtgttgaag
    21241 tttttgcagc ataaccgcgt cgacgccatt tttctggata tcaatattcc gtcgctggat
    21301 ggcgtgttgc tggcgcaaaa catcagccag ttcgcccata aaccgtttat tgtgttcatc
    21361 accgcgtgga aagaacatgc ggtagaagcg tttgaactgg aggcgtttga ctacattctc
    21421 aaaccgtatc aggagtcacg tattaccggg atgctgcaaa aactggaagc ggcctggcaa
    21481 caacagcaga ccagcagtac gcctgccgcg acggtaacgc gtgagaatga caccattaat
    21541 ctggtgaaag atgagcgaat aatcgtcacg ccaattaacg atatctatta cgccgaagcg
    21601 cacgagaaaa tgacctttgt ctatacgcgg cgtgaatcct acgtaatgcc gatgaacatt
    21661 accgaatttt gcagcaaact gccgccgtcg cattttttcc gctgccatcg ctcattttgt
    21721 gtcaatctga acaaaatacg cgaaatcgaa ccgtggttta ataacaccta cattctgcga
    21781 ctgaaagatc tggattttga agtgccggtc agccgcagca aagtgaaaga atttcgccag
    21841 ttaatgcatc tttaatcggg aactttcatg aaagcaccag gcttgcccgc cgatcaacaa
    21901 tttttcgccg atctgttcag cggcctggtg cttaactcgc aactactggg gcgcgtctgg
    21961 tttgccagcc agcctgcctc gttaccggtg ggcagtttat gtattgattt tccccgtctg
    22021 gatatcgtgc tgcgcggcga atacggcaac ctgctggaag caaagcagca gcgaatggtg
    22081 gaaggagaaa tgctgtttat tccggcacgc gcggctaatt taccgatcaa caacaaaccg
    22141 gtgatgctgt taagcctggt gttcgcaccg acctggcttg ggttatcgtt ttacgatagc
    22201 cgcaccacgt cgttgttgca tcctgctcgc cagatccagc ttcccagcct gcaacgcggt
    22261 gaaggtgaag cgatgcttac cgccctcact catcttagcc gttcgccgct ggagcagaat
    22321 atcattcagc cactggtgtt aagtttgctg catctttgcc gcaacgtggt gaatatgccg
    22381 ccgggtaatt cgcaaccgcg cggcgatttt ctctatcaca gcatttgtaa ctgggttcag
    22441 gataattata cccagccgct cacccgcgag agcgtggcgc agttttttaa tatcacgccc
    22501 aatcatctgt caaaactgtt tgctcagcat ggaacgatgc gttttatcga gtatgtgcgt
    22561 tgggtgcgaa tggcgaaggc gaggatgatt ttgcagaaat atcatctgtc aattcatgaa
    22621 gtggcacagc gttgcggttt tccggatagc gactattttt gtcgcgtttt ccggcgtcag
    22681 tttggtctga cgccgggaga gtacagcgcc cgttttcagg gctaacgtca gaaggttaat
    22741 tctgtttcca gcagcgtcag gatactttgt gcatcttgcg cggcaaacaa cgactggcgg
    22801 aagtttttat tcaccagttt acgcgccagc tgcgagaaga ctttcacatg attcatccct
    22861 tcgttagcac ccagcgtcag catgatcacc agttcgactt cgcccatttc tgactgccag
    22921 tcaatcggtt tcgccagccg ggcaatgctg atactggaat gacggatcca ctgagatttg
    22981 gtgtgcggga tcgctacgcc aaaaccaacg ccggtggtaa caatctcttc ccgctgccag
    23041 acatcttctt ccagctcaaa cggatgttca gtgcgcccgt taacgccgag gttgccgcac
    23101 aggaactgga tcgcctgctc tttattgcta aaatcctgat caacaaagat attctccagc
    23161 gccagcagtg ggcgaacgtc ttcttccggc gtaaaggcgg tgagtaacgc ttcaatttcc
    23221 tgcgcactgc ggcattcaca tgcctgacgc gccagttccc gacacgcctc gctatccagt
    23281 tgacgaagct ggcttttcac cgccggaata cgcgggctac tcatactcag ctcatccagg
    23341 cccagcccaa gcagtagcgg cagataacgg ctttcaccgc ccagttcacc gcaaatgcct
    23401 acccatttgc cccgctgatg cgcagtggta actatttgct gcaacatgcg caggaacgat
    23461 ggcgtaatcg ggttatatag cggcgatacg cgcgggttat tacgatcgac cgcatacaga
    23521 tactgggtca tatcgttgga gccgatactg aagaaatcga cctcatcgca gaagtggtcg
    23581 atgatgtagc acaccgacgg cacttccacc atgatcccaa gcgtaatcgt ctctgcatga
    23641 cgcaggccat cgcgcttaag ctcaacgatc gctttttgga tctcgccctt cacccataag
    23701 atctgatcga gactgtggac catcgggatc atcaactggg cgttgccaaa actggcggcg
    23761 cgcaaaatcg cccgtagttg agtgcggaac aggccagcaa attccgggta aatacgtacc
    23821 gcgcgatagc cgaggaacgg gttctcttcc tggggaatat tcagataagg aatgctttta
    23881 tcgccaccga tgtccattgt gcggaagata atcggcttgt cgcccgctgc cagtagcacc
    23941 tgctggtagg cttcaaattg ctcctgctcg tccggcgcgc tgtcgcgatc catatagagc
    24001 atttcagtac ggaacagtcc aacgccttcc gcgccgttgg caaacgcgcc tggcgcttcc
    24061 agagcggtgc cgatattcgc cgcaatatca atacgcttgt tatcacggga ataggccagc
    24121 tgcgcggcgg cctgcgcctg ttgtttttgg cgtttatccg ccagcgtctg tgcgacctga
    24181 taataaccgc tcaccgcgtc attcgggtta atcgccagca cgccgcactg ggcgtcaagc
    24241 accgccggtt gcccggcata acgggcaatc gcatccagcg gcaggccgct cagtaccggg
    24301 atcgccgagg cgcgggccag aatcagggta tgcgaggtgc gcccagtttt ctccagaatc
    24361 atgcctgcaa gattttttaa atcgaggctc aaaaactggc ttggggttaa atcctcggct
    24421 accagaatag tcggtttttc cagcaccaga ttgttgcgcg gcttcagttc cggccaggtg
    24481 atatgcagca actgttcgct aatatcgcga atgtcgctaa cacgttcgcg cagataatca
    24541 ctggcagaag cagaaagttt ggcgcaaacc tgctccatat tgctgatgat cgccgccccc
    24601 agcccttgat gctgttctgt catcagacga cggatattgc ccgcgaattc atcatcctga
    24661 atcagcgaca aatgggcgct aaggatagtt ttgctttcgc cgtcactctc acgcaattgc
    24721 tggttcagtt gctcggcaag cgttgcaagg ctgtgctcca gccgggtgga atcttgcgca
    24781 ctggcgggga ttgcccgata actgtcgagg ctgtcgcttt gtaacagggt cagcgtaccc
    24841 acgccgacgc tgcttgccag cacattgccg tacagtaaat ccgggttcag gcggcttaat
    24901 gaacgcggca gcggatgcgc cgtcagttct gcctgcgttg gctggatgct gtcgctgtcg
    24961 ataaagcgca cctggatgta ctcttccaac acgcgctgcg cctgctcttc atcgctgccg
    25021 ctaatgttca ggctgcaact gtcattaaat aaagtaccgg tgccaatcag cgccagcgag
    25081 cttttcgcat cggcctttgc gttctggcga tggttgataa aggtgatttc gctttgccac
    25141 tggctgcact gttctttaag ttcccacgcc ggacgagcat gaagaccgtt tggcaaagga
    25201 cagagaaatt gaatcgttaa cataaccgct ccgggttaat caggagatca tctgaaatcc
    25261 gtcagttgaa caaccgtctc acgcgtaaga cgtttaataa gggcagataa aagctgctgc
    25321 atctgcaaaa tgtcacggca atcggcaatc gatgcggcgc aatgtccgtg gcgggttgct
    25381 ggccccatca ccactgtagg cacgccggtg ccggttaagt gcaccgctcc gccgtccgtg
    25441 ccgccgttgc tgaacatatc tgcctgtaac ggcacgccaa tttctgccgc cacggtttcg
    25501 atccacgcgg taagttttgg cggcgcaatc agcgacttgt cgcttaacac cagcatcggc
    25561 ccgttacaaa tctgacgatg gttagccgcg ccataatcaa agtttttcgc ccagcaggcg
    25621 gtatcaagca caatggcgac atccggcgac accgcgcggg tggcagtttg cccgccgcgt
    25681 acccccacct cttcgctgga actggccacc agccacactt ccgcaggcag ttcagcgctg
    25741 tgtagttcgc gcagtaacgt caccagcagg tagcaaccga ggcggtcatc aaaggctttc
    25801 cccatcaccc gctggtgagg gagaacctga aaagttgtat caaaggtgac gcgatcgcct
    25861 ggacgaattc ccgcctgcat cacttcgtca tacgagcgcg taccaatgtc cacgcgcatg
    25921 gcgctgacgt cattcccctg ccggtcgccg tcaagcaggc ctggaatttt gcactcttca
    25981 cgggtggtga tgcgcaccgg ctgcagctgg cgggcagcca tgcgcacgtt gccaaccggc
    26041 agcacatcaa ttgccccttc gcgggagatg ctgcgcacca taaatcccac ttcgtccata
    26101 tgggcgcaga tcatcacctt cggccctgtc gattcattga ggcggatcag caccgatccc
    26161 agaccatcaa atcgcacttc tttttgcagg cgatccgctt cttccagcag gatctgccgc
    26221 acttcctgtt ccgaggaggc gatcgcatct gcctcgctca acgcttttaa tagcgataaa
    26281 tccattatgc ctctcccgtg agcaacactg ttttcggcat ggcgtagagc acttctgcgc
    26341 cttgcggggt aaccagcaca acatcttcga tgcgcacgcc cccttgccct ggcaaataaa
    26401 tccccggctc cacggtcagc aacatgcctg gctgtagcgt cgtggtgtcc cgcggtgaaa
    26461 aacgcggatc ttcatggact tcaatgccga tagcgtgacc agtgttatga ccgaaatagt
    26521 cgccaaatcc agcctcggta atgacccggc gcgcggcgtc gtcaatctgc tggcagcgca
    26581 cgccggggcg aattgcagag attgccgcga gctgtgcctg caggacaatc tgatagacgt
    26641 caaacagcgg gtgagattcg gcgctcaccc cttcgccatt caccagcaag gtgcgcgtca
    26701 tatcagagca gtagccctga tacagcgcac caaaatcgag agtgacaaac tcgcccgctg
    26761 caacaatctt gtcgctggct ttgccgtgcg gcagcacccc gcgccagccg ctggcgacaa
    26821 tggtgtcaaa agaggctttt tctgcgccct gctggcgcat aaaccactcc agttcagcgg
    26881 ctatctcgcg ctcgctcatc cccgcctgaa taaagcggcg aatatgctcc gctccgcgat
    26941 cggcaatccc acaggcgagg cggattatct ccacctcctc tggcgttttg atttgccgca
    27001 gcacatccgg cgtggcgcta accagtttcg cattgagttc agacttccag cggtgcgcgg
    27061 tttcccaact cacctgctgg ccttcaaaac cgagcgtttg caactgctca tcggcaatga
    27121 tttgattgac gatagtggta agcgtgttcg tcgcgtcaag caaatgcagc tggtagcctt
    27181 gcgcacgggc ttccacctca acgaaatagc tcgaatctac cagaatgtgc gcactttcac
    27241 ggctaataac cacatagcct gatccggtgg agatccccag atgcggctgt ttgttctgcc
    27301 gtgaggagag aagcactgca tccagttgtt gctccttaag ccagtcgcgc agcgaagcga
    27361 gtaatgtcat ctggattttc cttcttacag gctatcgatt aacaatttgc ctttacggaa
    27421 catcatcaga cgcaggaaca ccaccatcag cgcagttatg actgccccca gcgcgatccc
    27481 cgccatatag acgccaaggt tagtgaccag cggccatgcc cagatagcgg attccgggaa
    27541 ccattgcact gcgcccagcc agacagcggc ggttgagccg acaatcgcgc cgaccatata
    27601 cgacggaatg gcggtgatgg ggctttccag cgcaaacgga atcgcaccct cactaatccc
    27661 cataaaggca aggaacatcg cggtcttacc ctgcggataa agttgagcgt tgaacaggcg
    27721 tttgccggtt aaacgacggt caataatggt cgccagcccc agaccaatcg gaggaataac
    27781 gatagcgata gaacgagcgg tgaccggtaa tacgtggtca gtggtaaagc tgaaggcaac
    27841 gaaacctgcg gctttgttga tcgggccacc aaggtcgatt gctgtcgccg cggcgatccc
    27901 cattgcgtac atcagcgcgc ccttctcacc tgcggcagtc agcacggtac ggataacgcc
    27961 gttgatccag ccgccaaagg gggtaatgac gtagtacatc gccagcatga caaaaatggc
    28021 ggaaagaatc ggtagcaaaa atgtggtttt gaacgctaac aggaaatccg gtaactggat
    28081 tttctggttc atccacttca caaggtaacc cgcgacgata gaaataatca gcgcaccaat
    28141 gaaggtggac ggcaccggcg aagaggtcgc ccattgcata gtgctgggat cgaagttcag
    28201 cagttgcgtc ggctgggtag acatcaagcc gccgataaaa ccagccggga acgccagttt
    28261 gccaccgata gagttcgcca caaaagcagc aaacatcgga atggcaaaac caaacaacac
    28321 gccgccgaag gactgcgaca gccaggcaaa tttcagtaag gagaggtcga aaccggagaa
    28381 tttcccgcta ttaagagcat ccatgatgcc gatctcagca ggaattttaa gccagctata
    28441 agcaatcagc tggctgaaag cgaggatcac accgcccata attaaggtcg gcaccatgcg
    28501 tgaaatccct gacatcacat gctgcgggag ttcaccccag aagctgcttt tagaggcctg
    28561 cggattctta actgccgctg ccgcaccgga tgcgccaggc acaacggttg cactgcgttt
    28621 tttaatggcc ataacaatta tccctgtcga atattactgt tgttcagagg caatcatctc
    28681 ttcgatttct ttgatgatgc ccgcagcgtt tttaattgcg tcctgcaaag tgatttcata
    28741 aacgtcgcgt gattcgaaac gctcattatc ttccggggta actgccacgg agtggatgat
    28801 gatggttgct tcggcgatat cctgcgccgt caggcgattc tggataccgt ccgcgccctg
    28861 ggtttcaatt ttcacttcat aaccggcttc taccgccgct tcttccaacg cctgagcggc
    28921 cataaaggtg tgagccaggc ccattgggca ggcacataag gcaatcagtt tcttactcat
    28981 cgttaattcc tcattcagtg atagttgaca agtgaaatta tgaacgtgca aaacaaatcg
    29041 ccgttaccag acaaataatg catttactgg agaattaatc ccgctgtacg gaagtgtgac
    29101 gcaggtcggc cttgtgaggg gacacaaaga aaaattgaat taactgtatg atttaaacga
    29161 ttatcgggag agttacctcc cgatataaca ggaaggattt acagaatgtg ccctaaggtc
    29221 tggcgtaaat gtgcaccgga accgagaaga cccggattgt catggacgat gagatacacc
    29281 ggaatatcat ggacatattc tttaaagcgc cctttatctt caaatgcagc acggaaacca
    29341 gaggctttga agaactcaag gaagcgcggc acgataccgc ccgcaataaa cacgccgcca
    29401 aatgtcccga gattgagcgc cagattgccg ccaaaacggc ccataatgac gcaaaacagc
    29461 gacaacgcgc ggcggcaatc ggtacagctg tcagccagcg cgcgttcggt aatatctttt
    29521 ggcttgagat tttctggcag gcggttgtca gctttcacaa ttgcgcgata caaattcacc
    29581 agcccagggc cagaaagcac gcgctccgcc gaaacatgac caatttccgc acgcaatatt
    29641 tcgaggataa tggcctcttc ttcactattc ggcgcaaaat caacgtgacc gccttcgccc
    29701 ggcaagctta cccaacgctt atcgacatgg accagatgcg caaccccaag ccctgttccg
    29761 gcaccgtaaa ccgcaatagg tttaccttct accggttctg cgccaccaaa ctgaatcaga
    29821 tgctcttttt tcagcatcgg gatcgccatc gatacagcgg taaaatcgtt aataatttcc
    29881 agatggctaa aaccgagatt ctttttcatt tcggcaattg agaacgccca ggtatggttg
    29941 gtcattgcca cccagtcacc ggtaattggg caagcgatgg caatacagcc gtctttcacc
    30001 tcgaccttat gttcttcaag ataaacgcga atgaccgctt cgaggctggg gtaatcaagc
    30061 cctgaatagg tcttagcctg cgagatttca ccactggcaa tatcacacag agcaagacgt
    30121 gcgttggtgc cgcccacatc accgactaat gcatacttta tcattcttca actgctccgc
    30181 taaagtcaaa ataattcttt ctcacactgt aaatacctgg gggcataaca acaacgccgg
    30241 aaaggcaggc tccctgtaaa tatcgatctg ggtcacacat ttactttatc gtttcagcac
    30301 caattgcagc gatgcctttt tgcaagctgg gcaaactaag tatctgaccc cgcataagga
    30361 atagaacatg ctccatccgc gagccagaac catgttgtta ttatcgctcc ccgccgtggc
    30421 aattgggatt gcgtccagtc ttattctgat tgtggtgatg aaaatcgcct cggtattaca
    30481 gaatttgctc tggcaacgac tgccgggaac tctggggata gcccaggatt cacctctctg
    30541 gatcatcggt gtattaacgc taacgggtat tgcggtgggg ttggttatcc gtttcagcca
    30601 gggtcatgcc ggaccagacc ccgcctgtga accgctgatc ggcgcaccag ttccgccctc
    30661 tgcgctacca ggacttatcg tagcattaat tctcggtctt gctggcggcg tcagcctggg
    30721 gccggaacat ccgatcatga ccatcaatat cgcccttgcc gtcgccattg gcgctcgtct
    30781 gttaccgcgc gtcaaccgaa tggagtggac tattttagcc tctgccggaa ccatcggcgc
    30841 gctgtttggc acacctgttg cagcggcgtt gatattttcg caaaccttaa atggcagtag
    30901 tgaagttccg ctatgggatc gtctctttgc gccgttaatg gcggcagcag ctggtgcact
    30961 aactaccgga ttatttttcc atccccattt ttcactgccc attgcgcatt acggacagat
    31021 ggaaatgacc gatattctca gcggtgcaat tgtcgcggcg attgccatcg cagcagggat
    31081 ggtcgccgta tggtgcttac cacggttgca cgcgatgatg aatcaaatga aaaatccggt
    31141 gctcgtgctg ggtattggcg gatttattct cggtattctg ggggttattg gtggaccagt
    31201 ttcgctgttt aaagggctgg atgagatgca gcagatggtg gcaaatcagg ctttcagcac
    31261 cagcgattac tttttgctgg cggtaattaa acttgccgcc ctggtcgttg ctgccgccag
    31321 tggctttcgc ggtgggcgaa tcttcccggc agtgtttgtc ggcgtggcat tagggttgat
    31381 gctgcatgag cacgtacccg ccgtaccagc ggcaataacc gtttcttgcg ctattctcgg
    31441 catcgtgctg gtggtaacac gcgatggctg gttaagtctt tttatggcgg cggtcgttgt
    31501 acccaatacc acattgctac cgctgctctg tatcgtcatg cttccggcat ggctgttatt
    31561 agcaggtaag ccgatgatga tggtcaatcg tccgaagcaa cagccacccc acgataacgt
    31621 ttagcaaaaa atgctctcct ttatgattta agagttatgg cgatgatacg gggctttacc
    31681 tccccgtaat attgcgttaa caggccgctg acggcttcga tcgtgaagga gaataacaat
    31741 gttcaggtca ctgtttctgg cggccgccct gatggcattt accccgcttg cagcaaacgc
    31801 aggtgaaatc accctactgc catcaatcaa attacaaatt ggcgatcgcg atcattacgg
    31861 taattactgg gacggtggtc actggcgcga ccgtgactac tggcatcgca attatgagtg
    31921 gcgcaaaaac cgttggtggc gtcatgataa tggctatcat cgtggctggg ataagcgtaa
    31981 agcgtatgag cgtggctatc gtgaaggctg gcgcgatcgt gacgatcatc gcggaaaagg
    32041 ccgcggacat ggacaccgcc attaaacaca ttcgccaatg gagcacaatg cctgatgcga
    32101 cgctagcgcg tcttatcagg cctacaaacc ggatctttaa cgcgtagggc ggataaggcg
    32161 tttacgccgc atccgccaat agtgccagat gcgacgctca ttcaactaca atcccagcgc
    32221 cgtccccacc aacaaccaga tattcagcgc cacgaccagc actacaatca cccagcctgt
    32281 ctgttttacg cgtttgctgt tcaccagatc gcccatcaac ttgctgtcac tggtgaaaat
    32341 cagcagtgga accagcgcca gggcgatacc aaaacttaac agcacctgac tcataaccag
    32401 aatccgtgtc ggatctaatc ccatcagaat gacaataaat gacggcagca tggtgactgt
    32461 acgacgcacc cacagcggga tatggaagcg aatgaacccc tgcatcacca cctgccctgc
    32521 cagtgtcccc actaccgttg aggacagtcc ggcagcaacc agacttaacc caaagaccgt
    32581 tgccgcagca tggcttaaca gcggttgcag cgtcagataa gcctcatcaa gatcggcaac
    32641 accagtatga ccagaaaagt ggaacgccgc cgcagctgta gccatcatcg ccagattgac
    32701 aaaaccggca atagtcatgg cgatagccac atcccatttg gtggcggaat aacgttgttg
    32761 acgcgaaccg ccatgtaaat gctgagtgag cgaggagtgc aaataaatca catgcggcat
    32821 aatcgtcgcc cctaacacgc ctgctgccag gaagaccgct tccgaagtag gtaaactcgg
    32881 gatcaccatt cctttaccca gctgcgccag gttaggctgg gagaaaatca actcgacaat
    32941 gtaagccgcg gcaacaaaca acagtaaccc gccaatcact ttctccagcg gtttttgccc
    33001 gcgacgttgc agcattaaaa tcaggaaagt cgcgatcccc gtcagcaccg cgccctgcaa
    33061 caacgaaaca ccaagaatga gtttaaaacc gatcgccgca ccaataaatt ccgccaggtc
    33121 ggttgccatc gcaataattt ctgcctgaac ccaatagaac cacactacgg gacgcggata
    33181 gtgatcgcga atctgctccg ccagattttt accggtggca atccctagtt tggcagagag
    33241 gatctgaatc agcatcgcca tcaggttggc ccaaacgaca acccacagta gctgatagcc
    33301 gaagctggca cccgcctgaa tattggtcgc aaagttaccg ggatcgatat aaccaatcgc
    33361 cgcaatgaac gcaggtccca ttaatgcgag cctcatcttg cgcgccgccc gtccgctgct
    33421 actctcaacg cgatagttcg tcatcttgtg cctctaaaac atagcctttg ctatgtttca
    33481 tgctatgata aacgagaatg attatcaaat tcatttaaat ggattgtggt gatttctgtg
    33541 atagaccagg attatgacta cgaaaagatt gatgacttga atgttatggt tatgtttaat
    33601 gttagcacat ttacataact ttcagcttcc atacacaaca tagcagaaat gtatgacaga
    33661 tcactatttt tgaagcctgt cacaggacgt cattatagtg tgtgtcagat ctcgttttcc
    33721 ttaaccatgt tacatagaat gtgcacggaa atttaacctg cctcatattt ggagcaaata
    33781 tggaccgcgt ccttcatttt gtactggcac ttgccgttgt tgcgattctc gcactgctgg
    33841 taagcagcga ccgcaaaaaa attcgtatcc gttatgttat tcaactgctt gttatcgaag
    33901 tgttactggc gtggttcttc ctgaactccg acgttggttt aggcttcgtg aaaggcttct
    33961 ccgaaatgtt cgaaaaactg ctcggatttg ccaacgaagg gactaacttc gtctttggta
    34021 gcatgaatga tcaaggcctg gcattcttct tcctgaaagt gctgtgccca atcgtcttta
    34081 tctctgcact gatcggtatt ctccagcaca ttcgcgtgtt gccggtgatc atccgcgcaa
    34141 ttggtttcct gctctccaaa gtcaacggca tgggcaaact ggaatccttt aacgccgtca
    34201 gctccctgat tctgggtcag tctgaaaact ttattgccta taaagatatc ctcggcaaaa
    34261 tctcccgtaa tcgtatgtac accatggctg ccacggcaat gtccaccgtg tcgatgtcca
    34321 tcgttggtgc atacatgacc atgctggaac cgaaatacgt cgttgctgcg ctggtattga
    34381 acatgttcag cacctttatc gtgctgtcgc tgatcaaccc ttaccgtgtt gatgccagtg
    34441 aagaaaacat tcagatgtcc aacctgcacg aaggtcagag cttcttcgaa atgctgggtg
    34501 aatacattct ggcaggtttc aaagttgcca ttatcgttgc cgcgatgctg atcggcttta
    34561 tcgccctgat cgctgcgctg aacgcactgt ttgccaccgt gactggctgg tttggctaca
    34621 gcatctcctt ccagggcatt ctgggctaca tcttctatcc gattgcatgg gtgatgggtg
    34681 ttccttccag tgaagcactg caagtgggca gtatcatggc gaccaaactg gtttccaacg
    34741 agttcgttgc gatgatggat ctgcagaaaa ttgcttccac gctctctccg cgtgctgaag
    34801 gcatcatctc tgtgttcctg gtttccttcg ctaacttctc ttcaatcggg attatcgcag
    34861 gtgcagttaa aggcctgaat gaagagcaag gtaacgtggt ttctcgcttc ggtctgaaac
    34921 tggtttacgg ctctaccctg gtgagtgtgc tgtctgcgtc aatcgcagca ctggtgctgt
    34981 aagaccatac ataaaaaagc cggggataat tcc
//
LOCUS       DAJVUJ010000023        34755 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.023, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000023 DAJVUJ010000000
VERSION     DAJVUJ010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34755
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.023"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(72..1100)
                     /gene="malI"
                     /locus_tag="OQQ48_001051"
     CDS             complement(72..1100)
                     /gene="malI"
                     /locus_tag="OQQ48_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HCS1951898.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            1275..2867
                     /gene="malX"
                     /locus_tag="OQQ48_001052"
     CDS             1275..2867
                     /gene="malX"
                     /locus_tag="OQQ48_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HCS1951899.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQTQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPAAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYAIFRFAITRFNLKTPGRDSEVASSIEKAVAAAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            2877..4049
                     /gene="malY"
                     /locus_tag="OQQ48_001053"
     CDS             2877..4049
                     /gene="malY"
                     /locus_tag="OQQ48_001053"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HCS1951900.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDSQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCNMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRIYLKDNLTYIADKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            4152..5153
                     /gene="add"
                     /locus_tag="OQQ48_001054"
     CDS             4152..5153
                     /gene="add"
                     /locus_tag="OQQ48_001054"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HCS1951901.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLHYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNLARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRAGIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(5188..6228)
                     /locus_tag="OQQ48_001055"
     CDS             complement(5188..6228)
                     /locus_tag="OQQ48_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCS1951902.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            6441..6566
                     /gene="blr"
                     /locus_tag="OQQ48_001056"
     CDS             6441..6566
                     /gene="blr"
                     /locus_tag="OQQ48_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HCS1951903.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            6839..7054
                     /gene="ydgT"
                     /locus_tag="OQQ48_001057"
     CDS             6839..7054
                     /gene="ydgT"
                     /locus_tag="OQQ48_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HCS1951904.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            7140..7580
                     /locus_tag="OQQ48_001058"
     CDS             7140..7580
                     /locus_tag="OQQ48_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HCS1951905.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVSYFKRSSRVKATFVNP"
     gene            7657..8238
                     /gene="rsxA"
                     /locus_tag="OQQ48_001059"
     CDS             7657..8238
                     /gene="rsxA"
                     /locus_tag="OQQ48_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HCS1951906.1"
                     /translation="MTDYLLLFVGTILVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFTAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            8238..8816
                     /gene="rsxB"
                     /locus_tag="OQQ48_001060"
     CDS             8238..8816
                     /gene="rsxB"
                     /locus_tag="OQQ48_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HCS1951907.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            8809..11031
                     /gene="rsxC"
                     /locus_tag="OQQ48_001061"
     CDS             8809..11031
                     /gene="rsxC"
                     /locus_tag="OQQ48_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HCS1951908.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRTRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDARFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDPRKAAIE
                     AAIARAKARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAVPEEQVDP
                     RKAAVEAAIARAKARKLEQQQTNAEPEEQVDPRKAAVAAAIARAQAKKAAQQKVVNED
                     "
     gene            11032..12090
                     /gene="rsxD"
                     /locus_tag="OQQ48_001062"
     CDS             11032..12090
                     /gene="rsxD"
                     /locus_tag="OQQ48_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HCS1951909.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSTLLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGADMNTLHLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGLWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            12094..12714
                     /gene="rsxG"
                     /locus_tag="OQQ48_001063"
     CDS             12094..12714
                     /gene="rsxG"
                     /locus_tag="OQQ48_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HCS1951910.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            12718..13413
                     /gene="rsxE"
                     /locus_tag="OQQ48_001064"
     CDS             12718..13413
                     /gene="rsxE"
                     /locus_tag="OQQ48_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HCS1951911.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            13413..14048
                     /gene="nth"
                     /locus_tag="OQQ48_001065"
     CDS             13413..14048
                     /gene="nth"
                     /locus_tag="OQQ48_001065"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCS1951912.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            14660..16162
                     /gene="dtpA"
                     /locus_tag="OQQ48_001066"
     CDS             14660..16162
                     /gene="dtpA"
                     /locus_tag="OQQ48_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HCS1951913.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAIIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            16266..16871
                     /gene="gstA"
                     /locus_tag="OQQ48_001067"
     CDS             16266..16871
                     /gene="gstA"
                     /locus_tag="OQQ48_001067"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HCS1951914.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNETLKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(16915..17778)
                     /gene="pdxY"
                     /locus_tag="OQQ48_001068"
     CDS             complement(16915..17778)
                     /gene="pdxY"
                     /locus_tag="OQQ48_001068"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HCS1951915.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(17837..19111)
                     /gene="tyrS"
                     /locus_tag="OQQ48_001069"
     CDS             complement(17837..19111)
                     /gene="tyrS"
                     /locus_tag="OQQ48_001069"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCS1951916.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            complement(19240..19896)
                     /gene="pdxH"
                     /locus_tag="OQQ48_001070"
     CDS             complement(19240..19896)
                     /gene="pdxH"
                     /locus_tag="OQQ48_001070"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HCS1951917.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHAQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            complement(19955..20284)
                     /gene="mliC"
                     /locus_tag="OQQ48_001071"
     CDS             complement(19955..20284)
                     /gene="mliC"
                     /locus_tag="OQQ48_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HCS1951918.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVEHMQTATLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            complement(20382..21491)
                     /gene="anmK"
                     /locus_tag="OQQ48_001072"
     CDS             complement(20382..21491)
                     /gene="anmK"
                     /locus_tag="OQQ48_001072"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HCS1951919.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            21765..22232
                     /gene="slyB"
                     /locus_tag="OQQ48_001073"
     CDS             21765..22232
                     /gene="slyB"
                     /locus_tag="OQQ48_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HCS1951920.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(22279..22713)
                     /gene="slyA"
                     /locus_tag="OQQ48_001074"
     CDS             complement(22279..22713)
                     /gene="slyA"
                     /locus_tag="OQQ48_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HCS1951921.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            22914..23087
                     /locus_tag="OQQ48_001075"
     CDS             22914..23087
                     /locus_tag="OQQ48_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HCS1951922.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPL"
     gene            23153..24010
                     /locus_tag="OQQ48_001076"
     CDS             23153..24010
                     /locus_tag="OQQ48_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCS1951923.1"
                     /translation="MSIKTIKYFSIIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEK
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LRGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            24010..24477
                     /locus_tag="OQQ48_001077"
     CDS             24010..24477
                     /locus_tag="OQQ48_001077"
                     /inference="COORDINATES: protein motif:HMM:NF016513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCS1951924.1"
                     /translation="MNASSWSLRNLPWFKATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSA
                     WLGFCTWACAHFTNNVAYAFQLAATRLPSSPFRWLILLRPASCRISLRRAFAR"
     gene            24474..25154
                     /locus_tag="OQQ48_001078"
     CDS             24474..25154
                     /locus_tag="OQQ48_001078"
                     /inference="COORDINATES: protein motif:HMM:NF016513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCS1951925.1"
                     /translation="MIVGILCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWQ
                     PETNDAIRAAHEGGIGQILTMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISS
                     LRRMLLNWPSPPGATREILEQLLTALASSQTDAYTVARIIAPLRPTNVADYRHVAFWQ
                     RLRYFCRLYLQSSQELHRLQSGVDDHARLARTSALARHTDNAEAMWSGLRTFCTLMMI
                     GAWSIASQ"
     gene            25179..26021
                     /locus_tag="OQQ48_001079"
     CDS             25179..26021
                     /locus_tag="OQQ48_001079"
                     /inference="COORDINATES: protein motif:HMM:NF016513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCS1951926.1"
                     /translation="MAAISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQI
                     SDLWQFLLFLFPLLATMQLLKLQMPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLND
                     NLAKIVGVALAWLAFAILRPGSDARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTY
                     HHVSQLSNSQDALARRWLLRWGVVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRG
                     VMSERGVQQKSLAATLEELQRICDSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQG
                     TQAS"
     gene            complement(26022..26543)
                     /gene="sodC"
                     /locus_tag="OQQ48_001080"
     CDS             complement(26022..26543)
                     /gene="sodC"
                     /locus_tag="OQQ48_001080"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HCS1951927.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVITPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(26624..27520)
                     /locus_tag="OQQ48_001081"
     CDS             complement(26624..27520)
                     /locus_tag="OQQ48_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HCS1951928.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(27569..27808)
                     /locus_tag="OQQ48_001082"
     CDS             complement(27569..27808)
                     /locus_tag="OQQ48_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HCS1951929.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            27911..28510
                     /gene="nemR"
                     /locus_tag="OQQ48_001083"
     CDS             27911..28510
                     /gene="nemR"
                     /locus_tag="OQQ48_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416166.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HCS1951930.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYTAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            28547..29644
                     /gene="nemA"
                     /locus_tag="OQQ48_001084"
                     /pseudo
     CDS             28547..29644
                     /gene="nemA"
                     /locus_tag="OQQ48_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
     gene            29725..30132
                     /gene="gloA"
                     /locus_tag="OQQ48_001085"
     CDS             29725..30132
                     /gene="gloA"
                     /locus_tag="OQQ48_001085"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HCS1951931.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFMEDPDGYKIELIEEKDAGRGLGN"
     gene            30235..30882
                     /gene="rnt"
                     /locus_tag="OQQ48_001086"
     CDS             30235..30882
                     /gene="rnt"
                     /locus_tag="OQQ48_001086"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HCS1951932.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMIAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            30975..>34755
                     /locus_tag="OQQ48_001087"
     CDS             30975..>34755
                     /locus_tag="OQQ48_001087"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HCS1951933.1"
                     /translation="MADNPDPSSLLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARS
                     EHALVIAPTGSGKTLAAFLYALDRLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQLPLKGIADERRRRGETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGL
                     SATVRSASDVAAFLGGDRPVTVVNPPAMRHPQIRIVVPVANMDDVSSVASSTGEDSHA
                     GREGSIWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAVD
                     AAHFESTSGATSNRVQSSDVFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLEL
                     GIDMGAVDLVIQVATPLSVASGLQRIGRAGHQVGGVSKGLFFPRTRRDLVDSAVIVEC
                     MFAGRLENLTPPHNPLDVLAQQTVAAAAMEALQVDEWYSRVRRAAPWKDLPRRVFDAT
                     LDMLSGRYPSGDFSAFRPKLVWNRETGILTVRPGAQLLAVTSGGTIPDRGMYSVLLPE
                     GEEKAGSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSARLPF
                     WRGEGNGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTNANIQGLIDEQRNATG
                     IVPGSRHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASD
                     DGIVARIPDTDGKLPDAAIFLFEPEKLLQIVREAVGSSALFAARFRECAARALLMPGR
                     TPGHRTPLWQQRLRASQLLEIAQGYPDFPVILEILRECLQDVYDLPALERLMRRLNGG
                     EIQISDVTTTTPSPFATSLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVD
                     PGELLDPQVIRQVEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEV
                     ASYLENLLAVKRIFPAMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLR
                     DLFLRYLRAHALVTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRR
                     LRLRSLQAAREATRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMR
                     VIEQLAGVGLPASLWESQILPARVRDYSSEMLDELLATGAVIWSGQKKLGEDDGLVAL
                     HLQAYAAESFTPAEADQANRSALQQAIVAVLADGGAWFAQQISQRIRDKIGESVDLSA
                     LQEALWALVWQGVITSDIWAPLRALTRSSSNARTSTRRSHRARRGRPVYAQPISPL"
BASE COUNT         8073 a         9048 c         8917 g         8717 t
ORIGIN      
        1 cctgtgtgcc acatttttta tcattaacct tgaggaaagg acactaccag gtatagcaga
       61 taaagagagg attatttcgc tgcaatgagc cgggcgggaa taataagatt gcgtgaatgc
      121 gtctcttcat gggtgatttt ttgcatcatg cgatccgcaa gtgtggtacc aagttcccgc
      181 gcaggcgtgc tggcccaggt aacgggtata tcatcaagtg tggttggtgt cgcatcggta
      241 aatgccgcca gcgaaacttg ttgctcaaag taacgatcga ctccgctttc cccgctttgc
      301 cgccctgctt tcagcaaacc aaaccatgcc cccatcgcaa tagtttcgtt atagcaaacc
      361 acagcactga tggtcgggtt atgacgtaaa agcgccgtga tagcttccgc agcttgcttc
      421 tggctggatg tgcactccag cacccaatcg ctgtgaaacg gcagaccaaa ttttagtaga
      481 gttgcacaat agcctcccac ccgttctgcc cgggttaatg aggaactttg ccctcccagc
      541 caggcgatcc gctgatgccc attgcgaatg agatgctccg tcaacaactg tgcagcctgc
      601 atgttgtccg ggcgaaccgt atcaacatca tcaagataac tggcacggga agcgaaaatc
      661 accgggatag ctttttcttc tgccatccgt cgcaggtcat cgctgcttcc tgcagccccg
      721 gcaattacca caccatcgac cccctgattc agtaacagtg aaaaccgctg tgccagctgc
      781 tcgccgtctt taccgccgtg aagcaaaaaa accatccgtc cctgcgcttc cagagcttcc
      841 gtcaatccgg ccgtcaattc ggcgtaaaac ggcgcagata aatcacggac gatcaaacca
      901 atgacgccgc tttgcccgcc gcgcagcgcc gacgcctggc gattgcgcac aaatcccagc
      961 tcttcaatgg cggcgttaac gcgttctcct gtggcggtag agattcgccc tttgccacta
     1021 agcaccagcg aaacggtact taccgacacg cccgcagcca gcgcaacatc atgaatggtt
     1081 atttttttgg cggtagccat gcagatgacc tactccctga ttatgtgaca gataaaacgt
     1141 tttacctttt atttatctta tacccgctat tatcgttgcg taatgtgatt tatgcctcac
     1201 taaaatttga taaaacgttt tatcttctcg cgcaatttac tgaatccaga ttgttctcta
     1261 cgaggagtcg ttttatgacg gcgaaaacag caccgaaagt cacgctgtgg gagttcttcc
     1321 agcagttagg caaaaccttc atgttacccg tggcattatt gtcgttctgc ggcattatgc
     1381 tcggcattgg tagttctctt agcagccatg atgtcataac cctgatcccg gtcctgggca
     1441 accccgtgtt gcaggctatc tttacctgga tgagtaagat tggctcgttt gcttttagtt
     1501 tcctgcctgt catgttctgt atcgccatcc cgctgggcct ggcacgcgaa aataaaggcg
     1561 tagcggcatt cgctggcttc gtcggttatg cggtaatgaa cctcgcggta aacttctggt
     1621 tgaccaataa aggcattctg ccaaccacgg atgccgcggt tctgaaagcc aataacatcc
     1681 agagcattct tgggatccag tcgatcgata ccgggatcct cggtgcggtg atcgccggta
     1741 ttatcgtctg gatgctgcat gagcgtttcc ataatatccg cctgccggat gcgctggcat
     1801 tcttcggcgg tacgcgcttc gtaccaatta tctcctcgct ggtgatgggc cttgtcggcc
     1861 tggtgattcc attagtctgg ccgattttcg ccatgggtat tagcggcttg ggccatatga
     1921 taaacagcgc gggtgatttc ggaccgatgc tgtttggtac cggtgaacgt ctgctgttgc
     1981 cgtttggtct gcatcacatt ctggtggcat taattcgctt taccgacgca ggcggcacgc
     2041 aggaagtctg cggtcaaacc gtcagcggcg cactgaccat cttccagacg caattgagtt
     2101 gcccgaccac tcacggtttt tctgaaagcg ccacgcgttt cctttcgcaa ggtaaaatgc
     2161 ctgcgtttct cggcggtctg ccagctgcag cgttagctat gtatcactgc gcgcgcccgg
     2221 aaaatcgcca taaaattaaa ggtctgctga tttctggcct gatcgcctgc gtcgttggcg
     2281 gcactaccga accgctggaa ttcctgttcc tgttcgtagc gccagttctg tatgtcatcc
     2341 acgcgctgtt aaccggcctc ggcttcaccg tcatgtctgt gctcggcgtc accatcggta
     2401 ataccgacgg caatatcatc gacttcgtgg tgttcggtat tttgcatggt ctgtcaacca
     2461 agtggtacat ggtgccagtg gtggcggcaa tctggtttgt cgtttactac gccatcttcc
     2521 gtttcgctat cacccgcttc aatctcaaaa ccccggggcg cgatagcgaa gttgccagct
     2581 caatcgaaaa agccgttgcc gctgcgccgg gtaaatcagg ttacaacgtt cctgcaatcc
     2641 tcgaagcatt aggcggtgcc gacaatattg tcagcctcga taactgcatt acccgtctgc
     2701 gtttgtctgt gaaagatatg tcgcttgtta atgtgcaggc actgaaggac aatcgggcaa
     2761 ttggcgtagt acaacttaat caacataacc tgcaggttgt tatcgggcca caagttcagt
     2821 cagtaaaaga tgaaatggcc ggtctgatgc atactgtcca ggcataagga taagatatgt
     2881 tcgatttttc aaaggtcgtg gatcgtcatg gcacatggtg tacacagtgg gattatgtcg
     2941 ctgaccgttt cggcactgct gacctgttac cgttcacgat ttcagacatg gattttgcca
     3001 ctgccccctg cattatagag gcgctgaatc agcgcctgat gcacggcgta tttggctaca
     3061 gccgctggaa aaacgatgag tttctcgctg ctattgccca ctggttttcc acccagcatt
     3121 acaccgccat cgattctcag acggtggtgt atggcccttc tgtcatctat atggtttcag
     3181 aactgattcg tcagtggtct gaaacaggtg aaggcgtggt gatccacaca cccgcctatg
     3241 acgcatttta caaggccatt gaaggtaacc agcgcacagt aatgcccgtt gctttagaga
     3301 agcaggctga tggttggttt tgcaatatgg gcaagttgga agccgtgttg gcgaaaccag
     3361 aatgtaaaat tatgctcctg tgtagcccac agaatcctac cgggaaagtg tggacgtgcg
     3421 atgagctgga gatcatggct gacctgtgcg agcgtcatgg tgtgcgggtt atttccgatg
     3481 aaatccatat ggatatggtt tggggcgagc agccgcatat tccctggagt aatgtggctc
     3541 gcggagactg ggcgttgcta acgtcgggct cgaaaagttt caatattccc gccctgaccg
     3601 gtgcttacgg gattatagaa aatagcagta gccgcgatgc ctatttatcg gcactgaaag
     3661 gccgtgatgg gctttcttcc ccttcggtac tggcgttaac tgcccatatc gccgcctatc
     3721 agcaaggcgc gccgtggctg gatgccttac gcatctatct gaaagataac ctgacgtata
     3781 tcgcagataa aatgaacgcc gcgtttcctg aactcaactg gcagatccca caatccactt
     3841 atctggcatg gcttgattta cgtccgttga atattgacga caacgcgttg caaaaagcac
     3901 ttatcgaaca agaaaaagtc gcgatcatgc cggggtatac ctacggtgaa gaaggtcgtg
     3961 gttttgtccg tctcaatgcc ggctgcccac gttcgaaact ggaaaaaggt gtggctggat
     4021 taattaacgc catccgcgct gttcgttaac cccaattgcg caacgtaaaa atcgttgcgc
     4081 aatcgtggat ttttaccctg ctttgttttt ataatggtgc gcacttttat atccagaaaa
     4141 agagtgcgac catgattgat accaccctgc cattaactga tatccatcgc caccttgatg
     4201 gcaacattcg tccccagacc attcttgaac ttggccgcca gtataatatc tcgcttcctg
     4261 cacaatccct ggaaacactg attccccacg ttcaggtcat tgccaacgaa cccgatctgg
     4321 tgagctttct gaccaaactt gactggggcg ttaaagttct cgcctctctt gatgcctgcc
     4381 gccgcgtggc atttgaaaac attgaagatg cagcccgtaa cggcctgcac tatgtcgagc
     4441 tgcgtttttc accaggctac atggcaatgg cacataagct gcctgtagcg ggtgttgtcg
     4501 aagcggtgat cgatggcgta cgtgaaggtt gccgcacttt tggtgtgcag gcgaagctta
     4561 tcggcattat gagccgaacc ttcggcgaag ccgcctgtca gcaagagctg gaggcctttt
     4621 tagcccaccg tgaccagatt accgcccttg atttagccgg tgatgaactt ggtttcccgg
     4681 gaagtctgtt cctttctcac ttcaaccttg cgcgtgatgc gggctggcat attaccgtcc
     4741 atgcaggcga agctgccggg ccggaaagca tctggcaggc gattcgtgaa ctgggtgcgg
     4801 agcgtattgg acatggcgta aaagccattg aagatcgggc gctgatggat tttctcgccg
     4861 agcaacaaat tggtattgaa tcctgtctga cctccaatat tcagaccagc accgtagcag
     4921 agctggctgc acatccgctg aaaacgttcc ttgagcatgg cattcgtgcc ggcattaaca
     4981 ctgacgatcc cggcgtacag ggagtggata tcattcacga atataccgtt gccgcgccag
     5041 ctgctgggtt atcccgcgag caaatccgcc aggcacagat taatggtctg gaaatggctt
     5101 tcctcagcgc tgaggaaaaa cgcgcactgc gagaaaaagt cgccgcgaag taacaaaatg
     5161 gatggtgcaa attgcaccat ccatttttca tgcaaggcac aaagtcgcgc gatgtttggc
     5221 ggattcgatg cccagctcaa tcaactccat tacctggatt gcctggcttg ccggaaccgg
     5281 attttcacca tcgccattta acgcatcacg aatagccgca tagtaagccg gataattccc
     5341 aggtaccgtc aacagcgttt cttcgacacg ttcctcacct tccactcggg taagtacgcc
     5401 atcacgcata tcgtagcccc agtcttcctg cggcagacgc tcgccatttt tcagacgttc
     5461 ttcctgtgga tcgaggccat atttcacata actgcctcgg gatccatgca cgatataccg
     5521 tgctgactca gcggctgcca gcatggtacc gtgtaaaatg actcgccgct gtggatagga
     5581 caagatggcg tggaaataat cggtcgactg cgctccgggc cgtaactgtg ccaaatctac
     5641 cgtcatgctg accggtaaac caaatagcgt aatggcctga tcaagaagat gcggtgctaa
     5701 atcgtaccag ataccgctgc ctgggccgcc ctgttcacgc caacgatcgc gcacctgcgg
     5761 acggaagcgg tcaaaatgag actcaaagta agcaacttca cccagcacgc cttccgcgag
     5821 caaacctttt agcgtcaaga aatcgctatc ccaacgacgg ttatggaata cagacagcac
     5881 acgccccagg ctttttgcca gcgcatccag ctctcgcgct tgtgacagtg tcacggtaaa
     5941 gggtttatca acgaccacat gtttacccgc ctcaagcgcc gctttggcta acgggaaatg
     6001 ggtatcgttg ggtgtaggaa tgacaatcag gtctatgttg ggatcgttaa acagatgctt
     6061 cggctcagag acaaccgtaa ccgttggcca gtcggctttt acttttgttt catcactgct
     6121 ggagattacc gccagttcca gcccgggcgt gcccgcaatc aggggcgcat ggaaggtttt
     6181 gctcgcataa ccatacccaa tcaacccaac acggatgttg tcgctcatgt cattgcctct
     6241 cattgaagta tgattgctat ttgacactat cctttaccca cgctcaacag tttaatatta
     6301 agtcaaaaaa atagcgaatt ttccaacgac aaaagctaaa tatcgcaaaa acctcagtaa
     6361 aaatcttgct ggagctatta ttgctaagta acatttaccc cctgaagtta atggatcaat
     6421 caagagagat gtgggctgta atgaatcgtc ttattgaatt aacaggttgg atcgttcttg
     6481 tcgtttcagt cattcttctt ggcgtggcga gtcacattga caactatcag ccacctgaac
     6541 agagtgcttc ggtacaacac aagtaagctc tgcgcttgta gagcgccatg ctgcccgtcc
     6601 ggttgcatgt tttcacttgt cggatattaa accaggaatt tattatcttg ttcgatgttg
     6661 ttggtgattg tcagggatag taaagcgcgt tactcttttc agagcagttt ttcatccctg
     6721 aatatttgcc atcagcgcat tcttattatt gtaataacac aagcgttcac ttttggtgaa
     6781 attttatcat cactaataat acctacccct ttggtattaa ttattttatg gaccttttat
     6841 gactgttcag gactacttat taaaatttcg caaaatcagt tcactcgaaa gtctggaaaa
     6901 actctacgac catcttaatt acaccctgac ggacgatcag gaactgatca atatgtatcg
     6961 tgctgccgat caccgtcgcg cagagctggt ttctggcggg cgtttgtttg acctcggcca
     7021 ggtaccgaag tccgtctggc actatgtcca ataaagaaag tagcgatcgg ttataaaagc
     7081 cttataataa cgcccctaaa attagatttg cctgtttgcg tcgctattct ggagaaccta
     7141 tgaccaccac aacgccacaa cgtattggag gctggttgct tggcccgttg gcctggcttt
     7201 tagtcgctct gttgagtacg acgctggcgt tgttgctgta taccgctgcg ttatcttctc
     7261 cgcaaacatt tcaaacgctc ggcggacaag cactgacgac acaaatctta tggggcgttt
     7321 cttttattac cgctatcgcc atgtggtatt acacactgtg gttgaccatc gctttcttta
     7381 aacgtcggcg ttgtgttcct aaacactata ttatctggct gcttatttcc gtattgctgg
     7441 cagtaaaagc attcgctttt tcacccgtgg aagatggcat agccgttcgc cagttgctgt
     7501 ttactttgtt ggcaaccgca ttgatcgtct cctatttcaa acgctcgtcg cgggtaaaag
     7561 cgacgtttgt gaatccgtaa taaccttaca gttaacctgt tgtcgcctgc tctggattaa
     7621 cggataatag gcggcttttt tatttcaggc cgaaaaatga ctgactacct gttactgttt
     7681 gtcggaacta tactggtcaa taactttgtg ctggtcaagt ttctcggtct ctgtccgttt
     7741 atgggggttt ccaaaaaact ggaaaccgcg atgggcatgg ggctggcaac aacgtttgtg
     7801 atgacgctgg cgtctatttg cgcctggctt atcgatacgt ggattttgat cccacttaat
     7861 ctgatttacc tgcgcaccct ggcatttatt ctggtgattg ctgtggtcgt gcagttcacc
     7921 gagatggtgg tgcgcaaaac cagcccggtg ctttaccgtc tgctggggat ttttttgccg
     7981 cttatcacca ccaactgtgc agtgctcggc gtggcgttgc tgaatatcaa tctcgggcac
     8041 aatttcttgc agtcggcgct gtacggtttt tccgccgctg tcggtttctc gctggtgatg
     8101 gtgctcttca ccgccatccg cgaacgcctt gctgtggctg atgtcccggc accttttcgc
     8161 ggtaatgcca ttgcgttaat taccgcaggt cttatgtctc tggcctttat gggctttagt
     8221 ggtttggtga agttgtaatg aatgctatct ggattgccgt tgccgccgtg agcctgctgg
     8281 gcctggcgtt tggcgccatt ctgggttatg cctcccgccg ttttgcggtg gaagacgatc
     8341 cggtcgttga gaaaattgac gaaatcttac cgcagagcca gtgtggtcag tgcggttatc
     8401 ccggctgtcg cccctacgcg gaagccatca gctgtaacgg tgaaaaaatc aaccgttgcg
     8461 ccccaggtgg cgaagctgtg atgctaaaaa ttgccgagtt gcttaatgtc gagccgcagc
     8521 cgctggatgg cgaagcgcaa gagctaacgc ctgcgcggat ggtggcggtt attgatgaaa
     8581 ataactgtat tggctgcact aaatgtattc aggcttgtcc ggtagacgcc atcgttggcg
     8641 ctacccgtgc catgcatacg gtaatgagtg atctctgtac gggctgcaat ttatgtgttg
     8701 atccgtgccc gacgcactgc atctcgttgc aaccggtcgc agaaacacct gactcctgga
     8761 aatgggatct gaacaccatt cccgtgcgta tcattcccgt ggaacaccat gcttaagtta
     8821 ttctctgcat tcagaaaaaa taaaatctgg gatttcaacg gcggcatcca tccaccggag
     8881 atgaaaaccc agtccaacgg tacccccctg cgccaggtac cactggcgca gcgttttgtt
     8941 attccactga aacagcatat tggcgctgaa ggtgagttgt gcgttagcgt cggcgataaa
     9001 gtattgcgcg gccagccgct tacccgtggt cgcggcaaaa tgctgcctgt tcacgcgccc
     9061 acctcgggta ccgttacggc tattgcgccc cactctacgg ctcatccttc agctttagct
     9121 gaattaagcg tgattattga tgccgatggt gaagactgct ggatcccgcg cgacggctgg
     9181 gccgattatc gcactcgtag tcgcgaagag ttaatcgagc gcatacatca gtttggtgtt
     9241 gccgggctgg gcggtgcagg attcccgaca ggcgttaaat tgcagggtgg cggagataag
     9301 attgaaacgt tgattatcaa cgcggctgag tgcgagccgt acattaccgc cgatgaccgt
     9361 ttgatgcagg attgcgcggc tcaggtcgta gagggtattc gcattcttgc gcatattctg
     9421 cagccacgcg aaattcttat cggcattgaa gataacaaac cgcaggcgat ttccatgctg
     9481 cgcgcggtgc tggcggactc taacgatatt tctctgcggg tgattccaac caaatatcct
     9541 tctggcggtg ctaaacaatt aacctacatt ctgaccggga agcaggttcc acatggcggg
     9601 cgttcatccg atatcggcgt attaatgcaa aacgtcggca ctgcttatgc agtgaaacgt
     9661 gccgttattg atggcgagcc gattaccgag cgtgttgtaa ccctgactgg cgaagcaatc
     9721 gctcgcccgg gcaacgtctg ggcacggctg gggacgccag tgcgtcattt attgaatgat
     9781 gcccgattct gcccctctgc cgatcaaatg gtgattatgg gtggcccgct aatgggcttt
     9841 accttgccat ggctggatgt cccggtcgta aagattacca actgtctgtt ggctccctct
     9901 gccaatgaac ttggcgaacc acaggaagaa caaagctgca tccggtgtag cgcctgtgct
     9961 gacgcctgcc ctgcggatct tttgccgcaa cagttgtact ggttcagcaa aggtcagcaa
    10021 cacgataaag ctaccacgca taacattgct gattgcattg aatgtggggc ttgcgcgtgg
    10081 gtttgcccga gcaatattcc cctggtgcaa tatttccgtc aggaaaaagc tgaaattgcg
    10141 gctattcgtc aggaagaaaa gcgcgccgca gaagccaaag cgcgtttcga agcgcgccag
    10201 gctcgtctgg agcgcgaaaa agcggctcgc cttgaacgac ataagagcgc agccgttcaa
    10261 cctgcagcca aagataaaga tgcgattgct gccgctctgg cgcgggtgaa agagaaacag
    10321 gcccaggcta cacagcctat tgtgattaaa gcgggcgaac gcccggataa cagtgcaatt
    10381 attgcagcac gggaagcccg taaagcgcaa gccagagcga aacaggcaga actgcagcaa
    10441 actaacgacg cagcaaccgt tgctgatcca cgtaaaactg ccgttgaagc agctatcgcc
    10501 cgcgccaaag cgcgcaagct ggaacagcaa caggctaatg cggaaccaga agaacaggtc
    10561 gatccgcgca aagccgccgt cgaagccgct attgcccgtg ccaaagcgcg caagctggaa
    10621 cagcaacagg ctaatgcgga accagaagaa caggttgatc cgcgcaaagc cgccatcgaa
    10681 gccgctattg cccgagccaa agcgcgcaaa ctggaacagc aacaggctaa tgcggagcca
    10741 gaagaacagg ttgatccgcg caaagctgcc gtcgaagcgg ctattgcacg cgccaaagca
    10801 cgcaagctgg aacagcaaca ggctaatgcg gtacctgaag aacaggttga tccgcgcaaa
    10861 gccgccgtcg aagccgctat tgcccgtgcc aaagcgcgca agctggaaca gcaacagact
    10921 aacgcagaac cagaagaaca ggttgatccg cgcaaagcgg cagttgccgc ggctattgcc
    10981 cgcgctcagg ccaaaaaagc cgcccagcaa aaggttgtaa acgaggacta aatggtattc
    11041 agaatagcta gctcccctta tacccataac cagcgccaga catcgcgcat tatgctgttg
    11101 gtgttgctcg cagccgtgcc aggaatcgca gcgcaactgt ggttttttgg ttggggtact
    11161 ctcgttcaga tcctgttggc atcggttagt actctgttag ccgaagctct cgtactcaaa
    11221 ctacgcaagc agtcggtagc cgcaacgttg aaagataact cagcattgct gacaggctta
    11281 ttgctggcgg taagtattcc ccccctcgcg ccatggtgga tggtcgtgct gggtacggtg
    11341 tttgcggtga tcatcgctaa acagttgtat ggcggtctgg ggcaaaaccc gtttaatccg
    11401 gcaatgattg gttatgtggt cttactgatc tccttcccgg tgcagatgac cagctggtta
    11461 ccgccacatg aaattgcggt caacatccct ggttttatcg acgccataca ggttattttc
    11521 agcgggcata ccgccagtgg tgctgatatg aacacactac acttaggtat tgatggcatt
    11581 agtcaggcga caccgctgga tacatttaaa acctctgtcc gtgccggtca ttcggttgaa
    11641 cagattatgc aatatccgat ctacagcggt attctggcgg gcgctggttg gcaatgggta
    11701 aatctcgcct ggctggctgg cggcctgtgg ttgctatggc agaaagcgat tcgctggcat
    11761 attcccctca gcttcttagt aacgctggcg ttatgcgcaa cgttgggctg gttgttctca
    11821 ccagaaacac tggcagcacc gcaaattcat ctgctgtctg gtgcgaccat gctcggcgca
    11881 ttctttattt tgactgaccc ggttaccgct tctacgacca atcgtggtcg tcttattttc
    11941 ggcgcgcttg cgggcttatt agtctggttg atccgcagtt tcggcggcta tcctgacggc
    12001 gtggcttttg ccgtcctgct ggcgaacatc acggttcctc tgatcgatta ctacacgcgt
    12061 ccgcgcgtct acggccatcg caaagggtaa accatgctga aaactatccg aaaacacggc
    12121 attacgttag cgctatttgc agcgggttca acagggttaa ctgcggccat caaccagatg
    12181 accaaaacga cgattgctga acaggcaagt ctgcaacaaa aggcattatt tgatcaggtg
    12241 ctgccagccg aacgctataa caatgcgctg gcacagagtt gctatctggt aactgcgcca
    12301 gagttaggta aaggtgagca tcgggtttac atcgccaaac aggatgacaa accggtagcc
    12361 gccgttctgg aagcaaccgc gccagatggc tattccggtg cgattcagct gctggtggga
    12421 gccgatttta acggcacggt acttggcacg cgcgtgacag agcaccacga aacgccaggg
    12481 cttggcgata aaatcgaact gcgcctttct gactggatca cccattttgc gggtaaaaaa
    12541 atcagtggtg cggatgatgc gcactgggcg gtgaagaaag atggtggtga tttcgaccag
    12601 ttcaccggcg cgacgattac tccccgcgcg gtggttaatg cggtaaaacg cgccggattg
    12661 tacgctcaga cgttaccggc acaactttct caacttcctg cctgtggaga ataaagcgtg
    12721 agcgaaatta aagacgttat tgttcagggg ttgtggaaaa acaactctgc gctggtccag
    12781 ttgctcggcc tttgtcctct gttggcggtc acgtccactg ccactaatgc tctgggttta
    12841 ggacttgcga ctacgctggt actgacgctg accaacctga ccatttcgac gctgcgtcac
    12901 tggacgccag ccgagatccg cattcccatt tacgtgatga tcatcgcctc ggtggtcagc
    12961 gctgtacaga tgctgatcaa cgcctacgcc tttggcctgt atcaatcatt agggattttt
    13021 attccgctga ttgtcactaa ctgtatcgtt gtgggccgcg ctgaagcctt cgccgccaaa
    13081 aaaggtccgg cgctttcggc actggacggc ttttcaattg gtatgggcgc aacctgcgcc
    13141 atgttcgtgc tgggttcact acgcgaaatt atcggcaatg gcacattgtt tgacggtgca
    13201 gatgcgctgt taggtagctg ggcaaaagta ttacgcgtgg agattttcca caccgactcc
    13261 cctttcctgc tggcgatgct gccaccaggt gcatttattg gcctgggact gatgctggca
    13321 ggaaaatacc tgattgatga aagaatgaaa aagcgccgta ctgaagcagc tgccgaacgt
    13381 gcattgccaa acggtgaaac agggaatgtc tgatgaacaa agcaaaacgc ctggagatcc
    13441 tcactcgcct gcgtgagaac aatcctcatc ccaccaccga gcttaatttc agttcgcctt
    13501 ttgaattgct gattgccgta ctgctttccg ctcaggcgac cgatgtcagt gttaataagg
    13561 cgacggcgaa actctacccg gtggcgaata cgcctgcagc gatgcttgaa ctgggcgttg
    13621 aaggggttaa aacctatatc aaaacgattg ggctttataa cagcaaagca gaaaatatca
    13681 tcaaaacctg ccgtatctta ctggagcagc ataatggcga ggttccggaa gatcgtgctg
    13741 cgcttgaagc cctgcccggc gtaggtcgta aaacagccaa cgtcgtatta aacactgcat
    13801 tcggctggcc gactattgct gtcgacacgc acattttccg cgtttgtaat cgtactcaat
    13861 ttgcgccggg gaaaaacgtt gaacaggtag aagaaaagct actgaaagtg gttccggcag
    13921 agtttaaagt cgactgccac cattggttga tcctgcacgg gcgttatacc tgcattgccc
    13981 gcaagccccg ctgtggctct tgtattattg aagatctttg tgaatacaaa gagaaagttg
    14041 acatctgaag aaaaggggta acaccgatta ccccattgat aacctttctt tatcctcttt
    14101 taaaacatct ttttaacgtg aatgatgcca ttgcttagcg ttatcatcag gtaatccgtt
    14161 tgcggataac caaaaatgca ggttaattgt ttttttaata gcgaaatttt ctattcattc
    14221 gtgatcaaga tcacgatgct gtcgcaatgt aaaaacattg caagaatcag gttaaaatca
    14281 tttaaattta cacacgcaac aaatattgac ctacaaaaca ttacactggc tatttttcag
    14341 aaactggact atctcactag taaaaacgct aaatagcaga acatatcgcc gttaagccat
    14401 cacccccctc tcagtgcagt gaaaaaatct gccgttacgt tttttgaaaa atttaacgct
    14461 ggataacatt tcccggaatg gttgaattcc ccgcctcagt tatatgtaac agattattac
    14521 aaaggacttg tctgaaagtg caagatagtg aacattacct gccgtttccc ctcccactat
    14581 aacaattgcg cgtatgtttt ttagacataa cgcgagaaag cacccccgtt aatatgggat
    14641 gtaaaataaa gaggtaaaag tgtccactgc aaaccaaaaa ccaactgaaa gcgtcagttt
    14701 gaacgctttc aaacaaccga aggcgttcta tctcatcttc tcgattgagt tatgggaacg
    14761 ttttggttat tacggcctac aaggaattat ggctgtttac ctggttaaac aactgggtat
    14821 gtctgaagcg gattcaatca cccttttctc ttcctttagt gccctggttt atggtctggt
    14881 cgctatcggc ggctggttag gtgacaaggt actgggtact aaacgcgtaa ttatgctcgg
    14941 cgctattgtg ctggcgattg gttatgctct ggttgcctgg tctggtcacg acgccggtat
    15001 cgtttatatg ggtatggcgg ctattgcggt cggtaacggc ctgtttaaag ctaacccgtc
    15061 ttctctgctt tctacatgct atgagaaaaa cgacccgcgt ctggacggtg cattcaccat
    15121 gtactacatg tccgtcaaca tcggctcttt cttctctatg attgctacgc cgtggctggc
    15181 cgcgaaatac ggctggagtg ttgcgtttgc gttgagcgtt gtaggcctgc tgatcactat
    15241 cgttaacttc gccttctgcc aacgctgggt taaacagtac ggttcaaaac cagacttcga
    15301 gcctatcaac taccgtaacc tgctactgac cattattggt gttgtggcac tgatcgctat
    15361 cgccacctgg ctgctgcaca atcaggaagt tgcgcgtatg gcgctgggcg ttgttgcctt
    15421 cggtatcgtg gttatcttcg gtaaagaagc cttcgcgatg aaaggtgctg cgcgtcgtaa
    15481 aatgatcgtt gccttcatcc tgatgctcga agccattatc ttcttcgtgc tgtacagcca
    15541 gatgccaacg tcactgaact tctttgcgat tcgtaacgtt gagcactcca ttctgggtct
    15601 ggccgtagaa cctgagcagt atcaggcact gaacccgttc tggatcatca tcggtagtcc
    15661 gattctggcc gctatctata acaagatggg cgataccctg ccgatgccaa ccaagtttgc
    15721 aatcggcatg gtgatgtgtt ctggtgcgtt cctgattctg ccgctgggtg cgaaattcgc
    15781 gtctgacgct ggtatcgtgt ctgtaagctg gctggtcgca agctatggcc tgcagagcat
    15841 cggggaactg atgatctctg gtctgggtct ggcaatggtt gcacaactcg ttccgcagcg
    15901 tctgatgggc ttcattatgg gtagctggtt cctgaccact gccggtgcaa acctgattgg
    15961 tggttatgtt gcgggtatga tggctgtgcc ggataacgtt accgacccgc tgatgtcact
    16021 ggaagtttat ggtcgcgtat tcttgcagat tggtgtcgct actgccatta ttgcagtact
    16081 gatgctgctg accgcgccta aactgcaccg tatgacgcag gatgacgctg cagacaaagc
    16141 ggcgaaagct gctgtagcgt aaaatttagg gaaactcttt tacaagccgc taacttttcg
    16201 ttagcggctt tttttgttca gcaacgcact accatacttt aaaccacaga caaaaggagt
    16261 taccgatgaa attgttctac aaaccgggtg cctgctctct cgcttcccat atcaccctgc
    16321 gtgagagcgg aaaggatttt accctcgtca gtgtggattt aatgaaaaaa cgtctcgaaa
    16381 acggtgacga ttactttgcc gttaacccta aggggcaggt gcctgcattg ctgctggatg
    16441 acggtacttt gctgacggaa ggcgtagcga ttatgcagta tcttgccgac agcgtccccg
    16501 accgccagtt gctggcaccg gtaaacagta tttcccgcta taaaaccatc gaatggctga
    16561 attacatcgc caccgagctg cataaaggtt tcacacctct gtttcgccct gatacaccgg
    16621 aagagtacaa accgacagtt cgcgcgcagc tggagaagaa gctgcaatat gtgaacgaga
    16681 cactgaagga tgagcactgg atctgcgggc aaagatttac aattgctgat gcctatctgt
    16741 ttacggttct gcgctgggca tacgcggtga aactgaatct ggaagggtta gagcacattg
    16801 cagcatttat gcaacgtatg gctgaacgtc cggaagtaca agacgcactg tcagcggaag
    16861 gcttaaagta atagccatcg gcccacaggt cgttgtgggc cgaaatgaga tatttcagag
    16921 ctttgttgcg ctgaagtaat gttctggttt ggcaatacga tcctgagcag ccaccacttg
    16981 cagctcatat tcctgcattg ctttggtggt caccatgatt tcgtagactg cagcggtcac
    17041 atgttccagc gcctcctgca gcgttgcccc ctgaagcagt ttcaccagca gtaaaccgct
    17101 cgtcacatca ccaacaccta ccggctggcg cataccaaaa tccaccagcg gacggctgat
    17161 atgccaggct tcatcggcgg tgaccagcag catttcaaaa cggtcacggc tgtagccagc
    17221 tcgcgccagg tgtttaacca acacaatttg tggcccttgc gcaatgagtt cgcgcgctgc
    17281 cagaaccgct tcttcgacgt tatttaccgc atgctcacag agtatttcca gctcaaccag
    17341 attcggcgca atgatatcgc tggcaggcaa accgtgccgc acatgaaact ctgcgacacc
    17401 cggtgcaacg atacagcctt tttccggatg acccattacc ggatcgcaaa aatatttcgc
    17461 ctgcggattc gcggctttca cctgacggac gataccgagg atatgttcac cctgctccgc
    17521 cgatcccaga tagccactta acacggcatc acaggtgtgt aatttatcaa tggcggcaat
    17581 gccttgcaca atttcggtta aatggctggg cggcatcacg cagccagtcc atttgccgta
    17641 ctgggtgtga ttagaaaatt gaacggtgtt cagcggccag acgttcgcgc ccaggcggcg
    17701 cataggaaac tctgccgcac tgttacccgc atgaccataa acaacgtgag actggatagc
    17761 gagaatattt ttcatcatgt tccctgtata aaaaccaggg ggagtgattt ctcactcccc
    17821 ctttccactt aatgcattat ttccagcaaa tcagacagta attctttttg ccgcgacgca
    17881 gtaaggtaaa acgaccaaac agacgatctt cttctttaaa gaagtattca ggatcggact
    17941 gtttttcacc gttaatggtg atggcattgg aggcgatagt tttacgcgcc tgaccacggg
    18001 aaggttgcag ttcagaatcg accagcgcct gcatcaggtc tgcgcccttt tccatctcaa
    18061 ccatcggtac gccgtcctgc gccagctgtt cgaagtccgc ttcactcagc gcactcaaag
    18121 aaccgctgaa caggcattcg gtaatacgtt ttgccgcctg taaaccttct tcaccgtgaa
    18181 ccagacgagt cacctgctcc gccagtacat actgggcgcg cggtgcttta ccgctgtttt
    18241 tatcttcttc ttccagggcg ttgatctctt caatgctcat aaaggtgaag aacttcagga
    18301 agcggtaaac gtcggcatcc gcagtgttga tccagaactg gtagaatttg tacgggctgg
    18361 ttttcttcgg atccaaccag actgcgccgc cttcagtttt accaaatttg gtgccatctg
    18421 ctttagtgat cagcggaacg gtcaggccaa acacctgatt ctgatgcaga cgacgggtca
    18481 ggtcgatacc agaagtgatg ttaccccact ggtcagaacc accaatttgc agcaccacac
    18541 cgtactgttt gttcagacag gcgaagtcat aaccctgcaa caggttgtag gaaaactcag
    18601 tgaacgaaat cccctgatct tcacggttga gacgctgctt aaccgcttct ttgttgatca
    18661 tctggttaac ggagaagtgt ttgccaatat cgcgcaggaa ggtcagcaca ttcatattgc
    18721 cgaaccagtc atagttgttc gccgcgatag cagagttttc tccacagtcg aaatcgagga
    18781 acggggcaac ctgcttacgg attttgtcca cccactcctg aacagtttct tcggtgttca
    18841 gcttacgctc ggcagctttg aagctcgggt cgccaatcag acccgtcgcg ccgcctacca
    18901 gcgcaaccgg cttgtggccc gcctgttgga agcgtttcag gcataacaat ggaacaagat
    18961 gccccaaatg caagctgtca gcggtaggat cgaagccgca atagagcgcg atcgggcctt
    19021 gcgccagtcg ctctgctaac gcttcctcgt ccgtcacctg ggctaccagc ccccgctctt
    19081 gcaattgttt aatcaagtta ctgcttgcca tcaaaatctc catgtatata acgactgcac
    19141 ctttgccggt acacgacttt tcgccagatg cgaaagagac atagaataaa gtgccagaat
    19201 caggagtacc agcgattaaa gcaagatttt tgcatctttt cagggtgcaa gacgatcaat
    19261 cttccacgca tcattttctc gctggtacaa aaagcggtca tgcaggcgat gctcaccgcc
    19321 ctgccagaac tcaatctgtt caaggctgac gcgaaaaccg ccccaaaagc tcggcaatgg
    19381 cacttcgccc tgttgaaact tctgcttcag ctccaggaat ttactttcaa ggataccgcg
    19441 ggcagaaatg cgactggact gcttcgaaac ccatgcacca atctggctat cacgcgggcg
    19501 gctatgaaaa tatttcatca cttcgagagt cgaaagtcgt tctgctttac cgatcaccat
    19561 cacctggcgt tcaagggtat gccacgggaa cagcaggcta acgcgcggat tattttcaat
    19621 ttgatgtgct ttacggctac cgaggttggt gtaaaacacc atgccttttt cgtcgtaatg
    19681 tttgagtaaa acgatgcgtt gataaggctg agcatgttca tccacggtag cgaccaccat
    19741 cgcggtaggg tctgccagtt tggcttcaca cgcctgagag agccaacgtt caaaaagggt
    19801 taatggatcg gcgggaagat cgcgacggcg taacccgcct ttggtgtatt cacggcgcag
    19861 atgcgcgatt tgctgcaatt cgtcgttatc agacatggtt ttctttacgg attgtcagtg
    19921 ggtgacgcta ttgtgcgccg cccctggaaa aatctcaacg ctgtggattt tgtaactgac
    19981 agttattcaa tacgatgcgg tcgcgtttat agacagtcgc ttcatcgcct ttcgaccaga
    20041 aaacatagat tccgtcagtg taacgtgcac cagacgctga aatgccctgt ttgagatgca
    20101 gtagttgatt atcgtaaaca aaactgactt cctggcgcgg attattcagt ttgaccgtca
    20161 acggtttttc atcacactgg tattccagcg tagcggtttg catgtgctca accagttgat
    20221 tgaacgcgct acagccggtg agaagcaccg gcaaacagat cagtaacagt tttttcatag
    20281 acatttcccg aagactttcc tggtctggag ggcaatacgc cctccctaac gttccaagtg
    20341 taacggcaga cgcggtaaga aaaattcagt taactctgat atcacgggtt agcagggaaa
    20401 atagccccca gtaccgtctc ctggcttgcg ccagtgacgg aaggcagatt tcctggtaat
    20461 cccgccagcg tccgccaggc aagccaggcg aaagccaatg cttccatgtc atcgccacta
    20521 atgccaacgg catcggtggt ggtgacttct gtgcctggca gtaatgccgc cagacgcgcc
    20581 atgagtagcg gattacgact acctccacca cataccatca atcgttcgca gccaccgctc
    20641 aacaaaactt gttcagaaat ggtcacggcg gtgagttctg ccagtgtggc ctgaacatca
    20701 cggggatcaa cacccggaaa atggcgtaaa tggcgctcca gccagccgta gttaaagtat
    20761 tcgcgtccgg tgcttttcgg tgcaggttgc gagaaatatg ggtcgctgag catattttgc
    20821 agcagtggga gaataacttt acccgcccgt gcccactcgg catctttatc gtaaggttta
    20881 ccggcctgac gccagatcca ggcatccatc agcatgttac caggaccggt atcgtagccg
    20941 ccaaccggct gcccaggaat gagcagtgac agattggcga tgccgccaat attgagcacc
    21001 attcgtcgct cggttgggtg agccagcaag gcatgatgga acgcaggtac cagcggtgcg
    21061 ccttgtcctc ccaaggcaat atcgcgacgg cgaaaatcac cgacaaccgt aattccggtg
    21121 cgtgccacaa tttgattgtt atcgccaatc tgtaaagtgt gtggtgccac gcccgtcggt
    21181 tcatgccaga cggtttgacc gtgacagccg atcgcaacta tatctctcgc ctgcaggttt
    21241 tgttccttaa gcaaggcatt gaccgcatca gcaaaaagtc gcccgagttg agtatcaagc
    21301 tgtccaaact gcgaaagtgt aagctgctgg ccctggcaaa tatccagtac agcctgtttc
    21361 agagacaccg ggatcggcca gctcaagctt gccagctgtg cgacccggtg ttcatctatt
    21421 gtcgccaaca caacatcaac accatcaagg ctggtgcctg acataacgcc aataaagcgg
    21481 cccgatttca tagttcatcc tttttcaatc tgacgtttgc gccccactca aacataaact
    21541 tttcgtgaat accatgcgga atgaccgatt tttaccgttg gtagtaaaac attatcttca
    21601 aatcaataat catcatgaat gttttgttta taattggttg atcctacttt cattatgatt
    21661 tgctcatatt tggtagaaca tgtaaccatg gattcacata tgccatatac tttgaccatg
    21721 agggatgctt gcgtggcgtt tcatggtgaa caggagattt ttcaatgatt aaacgcgtat
    21781 tggttgtttc aatggtaggt ctgtctcttg tcggttgtgt taataacgac accctgtcag
    21841 gggatgttta taccgcttct gaagcgaaac aagtacagaa tgtcagctat ggcaccatcg
    21901 ttaacgtacg tccggtacag attcagggcg gtgatgattc caacgttatc ggtgcaattg
    21961 gcggtgctgt tcttggtggt ttcctgggga atactgttgg tggcggaacc gggcgttctc
    22021 tggctactgc agcaggcgct gttgcaggtg gcgtagctgg tcagggcgta cagagtgcaa
    22081 tgaacaaaac gcagggtgtc gagctggaaa ttcgtaaaga cgatggtaat accatcatgg
    22141 tggtacagaa acaaggcaac actcgtttct ctccgggcca acgtgtcgta ctggccagca
    22201 atggcagtca ggtgaccgtt tctccgcgct aaataagttt gcgtgtggtc aggttactga
    22261 ccacacgccc ccttcatttc accctttggc ctgtaactca atgatattat gctcaagttt
    22321 tgcgatgagc gtaatcagtt gctccagttc ctctgcggag atgccatgta atatttccgc
    22381 gcgggttttg ttaataacag cttccatttc gctgatcagt ggctctgcct tttccgtcag
    22441 tttaatacgt ttagcccgac gatcgctggc acaagtttga cgcgaaatta accctttttc
    22501 ttccagttgg tccagagtac ggaccagtga tggctgctcg atgccaatcg cttttgccag
    22561 ttgaatttgc gactggtctg gaggtaactg atggatattg tgtaacgtaa cccaatgggt
    22621 ttgtgttaac tccagcggtt tcaggcgatg gtctatcaga gcacgccata tgcgcaccaa
    22681 ccgtgccaga tcagaaccta gtggcgattc caatttcatc tccttataat tagcttgcta
    22741 agatattatg cggcttttag aatagtgtgc agcaatttta ttgctaaaac aaatgtattg
    22801 ctgcatttgg ttaccgtcag acatattttt cagaaattgc gcgtaaattt ttcgcactta
    22861 aagaatattt attaatctaa cgcaatatat tcggtcgtaa aggaatctac tttgtgaagt
    22921 ttatgctcaa tgcaacagga ttgcccttac aagatctggt gttcggtgcg tccgtctatt
    22981 ttcctccgtt tttcaaagcc ttcgcgtttg gattcgtcat ctggcttgtc gtacaccgcc
    23041 tgcttcgtgg ctggatctac gccggtgaca tctggcatcc cttgtaaatg gatttatcgc
    23101 tgtttgcgat ttgcgtttgc cttgctctgg caatactgat tgcgtggtaa ctatgtcaat
    23161 taaaacaatt aagtatttct caataattat tgtagcgata gttgcggttc ttgccggatg
    23221 gtggctgtgg aattattaca tgcaatctcc gtggacacgc gacgggaaaa tacgcgcaga
    23281 aaaggtttct atcaccccac aggtgtcggg acgtattgtt gagctgaata taaaagacaa
    23341 ccaactggtt aacgcggggg atcttctgct cacgatcgat aaaacgccct ttcagatcgc
    23401 cgagctgaac gcacaggctc agctggcgaa agcgcaatct gacctcgcaa aagccaataa
    23461 cgaagcgaat cgtcgtcgtc atctctcaca aaattttatt tctgccgaag aactcgatac
    23521 cgctaacctc aatgttaaag cgatgcaggc cagtgttaat gccgcacagg ccacgctgaa
    23581 acagacacaa tggcaactgg cgcaaacgga aattcgcgcc ccggtgagtg gttgggtgac
    23641 taacctcact acccgcatcg gcgattacgc tgacaccggg aaaccgctgt ttgcccttgt
    23701 cgacagccac tcgttttacg tcattggtta ttttgaagaa accaagttgc gccatatccg
    23761 cgaaggtgca ccagcacaaa ttaccctcta tagtgataac aaaacgttac ggggtcacgt
    23821 ttccagtatc ggtcgggcga tttatgatca aagcgttgaa agtgactcaa gccttattcc
    23881 ggatgtgaaa cctaacgtcc cctgggttcg tctcgcccaa cgcgttcccg tccgctttgc
    23941 gctggataaa gttccaggcg atgtcacgct ggtgtcgggc accacctgta gcattgccgt
    24001 aggtcaataa tgaacgcatc gtcatggtcc ttgcgcaatt tgccctggtt caaggccacg
    24061 ctggcgcaat ggcgttatgc gttacgcaat accattgcca tgtgtctggc gctgacggtt
    24121 gcctattatt tgaatctgga tgaaccctat tgggcgatga cctcggctgc agtggttagc
    24181 tttcccaccg ttggcggtgt catcagcaaa agcctcggac gcatcgctgg cagtttactg
    24241 ggagccattg cggcactgct tcttgccggg catacgctca atgagccgtg gttttttcta
    24301 ttgagcatgt cggcgtggct tggcttttgt acctgggcct gtgcgcactt cacgaataac
    24361 gtcgcgtatg catttcaact ggcggctaca cggctgccat catcgccttt ccgatggtta
    24421 atattactga ggccagccag ctgtaggata tcgctcaggc gcgcgtttgc gaggtgatag
    24481 tcggtatttt gtgcggcggc atgatgatga tgatcctgcc tagcagttcc gatgctacag
    24541 cccttttaac cgcattgaaa aacatgcacg cccgattact ggaacatgcc agtttactct
    24601 ggcagcctga aacaaacgat gccattcgtg cagcacatga gggggggatt gggcagatac
    24661 tgaccatgaa tttgctgcgt atccaggctt tctggagcca ctatcgtttt cgccagcaaa
    24721 acgcgcgcct taatgcgcta ctccaccagc aattacgtat gaccagtgtc atctccagcc
    24781 tgcgacgtat gttgctcaac tggccctcac cgccaggtgc cacgcgagaa attctcgaac
    24841 aattgctgac ggcgctcgcc agttcgcaaa cagatgctta caccgtcgca cgtattatcg
    24901 ccccgctacg cccgaccaac gtcgccgact accggcacgt cgccttctgg cagcgactac
    24961 gttatttttg ccgcctttat ctgcaaagta gtcaggaatt acatcgtctg caaagcggtg
    25021 tagatgatca tgccagactc gcacggacat ccgctctggc tcgtcatacc gacaacgccg
    25081 aagctatgtg gagcgggctg cgtacatttt gtacgttgat gatgattggc gcatggagta
    25141 ttgcatcgca ataggatgcc ggtgccaatg cattaacgct ggcagcaatt agctgcgtac
    25201 tctactccgc cgtcgcagca ccgtttaagt cgttgtcact gctgatgcgc acgctggtgt
    25261 tactttcgct attcagcttt gtggtcaaat ttggcctgat ggtccagatt agcgatctgt
    25321 ggcaattttt actgtttctc tttccactgc tggcgacaat gcagcttctt aaattgcaga
    25381 tgcccaaatt tgccgcactg tgggggcaac tgattgtttt tatgggttcg tttatcgctg
    25441 tcactaatcc cccggtgtat gattttgctg actttcttaa cgataatctg gcaaaaatcg
    25501 ttggcgttgc gctggcttgg ctagcgttcg ccattctgcg tccaggatcg gatgctcgta
    25561 aaagccgccg ccatattcgc gcgctgcgcc gggattttgt cgatcagcta agccgccatc
    25621 caacactgag tgaaagcgaa tttgaatcgc tcacttatca tcacgtcagt cagttgagta
    25681 acagccagga tgcgctggct cgccgttggt tattacgctg gggtgtagtg ctgctgaact
    25741 gttctcatgt tgtctggcaa ttgcgcgact gggaatcgcg ttccgatccg ttatcgcgag
    25801 tacgggataa ctgtatttca ctgttgcggg gagtgatgag tgagcgtggc gttcagcaaa
    25861 aatcactggc ggccacactt gaagaattac agcggatttg cgacagcctt gcccgtcatc
    25921 atcaacctgc cgcccgtgag ctggcggcaa ttgtctggcg gctgtactgc tcgctttcgc
    25981 aacttgagca agcaccaccg caaggtacgc aggcctctta attacttaat tacaccacag
    26041 gcatagcgtt caccgccacc gcccagcggt ttaggttgat cggacatatt atcgccgcca
    26101 acgtggacca tcagcgcttt gtctttgatt tcatccagtg atttcagacg aggcgtgatg
    26161 acggcatcgg tagctttgcc gtcattattg acgaccagtg caggcagatc gcctaaatgc
    26221 ccggcacctt ctggcccttc atgtttaccg gtattttgtg gatcaagatg cccgcctgcg
    26281 gattccgcgg cgctggcttt gccatctttg gtggctggct ggcagcttcc tttggcatga
    26341 atatggaagc catgttcacc ggggggtaat gctttcagat cgggcgaaaa ctccagacct
    26401 ttatcggttt cagtaatggt gacgctacca attgactgcc ctaccccttg cgacgtgacg
    26461 aggttcatct cgactttttc actggcagct tgtgcgccgg ttgcaacaac cagcgccaga
    26521 atagccagac taaaacgttt cataggacct ccgttcattg tgcattcctg aataagtgta
    26581 aaccagtgac acatttttga acggcggcta ttcctaaaag tgcttacggt acgtcgtacc
    26641 ccagtgccgc tttacgaata cgaaaccatt gttgacgggt cattttcagt gtttctgctt
    26701 cgacagctgc ccgtacgcgc tcaattttac ctgaaccgat aattggcagc ggctgcgatg
    26761 gtaaacgtaa tacccaggcg taaaccacct gttcaatcga gcccgcgttt aactcctctg
    26821 ccaccacagc cagttcatca cgcagcggct ggaaataatc atcattaaac agacgaccac
    26881 caccaaggca ggaccacgcc atcggacgaa cacgcagttg ttgtagttgg tcgagcgtgc
    26941 catccagcag taacggctga tgcaccgggg atatttccac ctgattagtg gcaagggtaa
    27001 acggcagacg tgattgcaac agggcaaatt gcgcaggcgt aaagttcgat acgccaaaat
    27061 gacgcacttt gccgctctga tgcagatgtt tgaacgcgtc cgccacttca tcggcatcca
    27121 ttaacgggtc tggtcggtgg attaacagca gatccagatg atcggtcgcg agattaatta
    27181 gcgactgttc ggcgctctta atgatgtgat cgcggtcagt gatgtaatga ccaatgacgt
    27241 tttcttcacg cgcggtcgtc gcgataccgc atttactgac gatttccatc cgttcacgca
    27301 ggtgaggtgc cagtttcagt gcctcgccaa acgccgcttc gcactgatag ccaccataaa
    27361 tatcagcatg gtccacggtg gtcacgccga gatccagatg ctcttcaata aaactgacca
    27421 gctggcgggc ggacatattc cagtccatca atcgccagta gcccatcaca aaacgggaaa
    27481 actccgggcc ttgcggcgca atagtaatac gctgaaccat aatcgcttcc tcttatcaga
    27541 tatgagagga gtatacgcaa gattaggttc aaaagagtga tggttgctcc ggttcgtctg
    27601 atgacgctgg cttatttgcg cgtaatttgc gcattaatcg ctgccgacaa aggcgcagca
    27661 cctcttgttt ttcgccatcg ctcattttat tccagttaaa acgctcatcc cgactacgaa
    27721 aacagccgcg acaaaacccg cgttcgtcag actggcaaat tccccggcac gggctctgga
    27781 cgggaaagaa ctctaattgc tccgccactt cgccctcctc agataagatt attaccatta
    27841 ttgaagctgt taatgtccaa agtagcaact ttgcttgcac tagaccgact ggtctactac
    27901 actccaacgc atgaacaaac acaccgaaca tgatactcgc gaacatctcc tggcgacggg
    27961 cgagcaactt tgcctgcaac gtggattcac cgggatgggg ctaagcgaat tactaaaaac
    28021 cgctgaagtg ccgaaagggt ccttctatca ctactttcgc tctaaagaag cgtttggcgt
    28081 tgccatgctt gagcgtcatt acaccgcata tcaccagcga ctgactgagt tgctgcaatc
    28141 cggcgaaggt aactaccgcg accgcatact ggcttattac cagcaaacac tgaaccagtt
    28201 ttgccaacat ggaaccatca gtggttgcct gacagtaaaa ctctctgccg aagtgtgcga
    28261 tctgtcagaa gatatgcgca gcgcgatgga taaaggtgct cgcggcgtga tcgccctgct
    28321 ctctcaggcg ctggaaaatg gccgtgagaa ccattgttta accttttgtg gcgaaccgct
    28381 acaacaggca caagtgcttt acgcactatg gctgggtgcg aatctgcagg ccaaaatttc
    28441 gcgcagtttc gagccactgg aaaacgcgct ggcccatgta aaaaacatta ttgcgacgcc
    28501 tgccgtttag caggcatttt ttatcaccag acgaccggga gcctttatgt catctgaaaa
    28561 actgtattcc ccactgaaag tgggcgcgat cacggcggca aaccgtattt ttatggcacc
    28621 gctgacgcgt ctgcgcagta ttgaaccggg tgacattcct accccgttga tggcggaata
    28681 ctatcgccaa cgtgccagtg ccggtttgat tattagtgaa gccacgcaaa tttctgccca
    28741 ggtaaaagga tatgcaggtg cgcctggcat ccatagtccg gagcaaattg ccgcatggaa
    28801 aaaaatcacc gctggcgttc atgctgaaaa tggtcatatg gccgtgtagc tgtggcacac
    28861 cggacgcatt tctcacgcca gcctgcaacc tggcggtcag gcaccggtag cgccttcagc
    28921 acttagcgcg ggaacacgta cttctctgcg cgatgaaaat ggtcaggcga tccgtgttga
    28981 aacatccatg ccgcgtgcgc ttgaactaga agagattcca ggtatcgtca atgatttccg
    29041 tcaggccatt gctaacgcgc gtgaagccgg ttttgatctg gtagagctcc tctctgctca
    29101 cggttatttg ctgcatcagt tcctttctcc ttcttcaaac catcgtaccg atcagtacgg
    29161 cggcagcgtg gaaaatcgcg cacgtctggt actggaagtg gtcgatgccg ggattgaaga
    29221 atggggtgcc gatcgcattg gcattcgcat ttcgccaatc ggtactttcc agaacacgga
    29281 taacggcccg aatgaagaag ccgatgcact gtatctgatt gaacaactgg gtaaacgcgg
    29341 cattgcttat ctgcatatgt cagaaccaga ttgggcgggg ggtgaaccgt atactgatgc
    29401 gttccgcgaa aaagtacgcg cccgtttcca cggtccgatt atcggcgcag gtgcatacac
    29461 agtagaaaaa gctgaaacgc tgatcggcaa agggttaatt gatgcggtgg catttggtcg
    29521 tgactggatt gcgaacccgg atctggtcgc ccgcttgcag cgcaaagctg agcttaaccc
    29581 acagcgtgcc gaaagtttct acggtggcgg cgcggaaggc tataccgatt acccgacgtt
    29641 gtaatccaac attgcgagcg gcgtaaagcc gccgctatac taaaacaaca ttttgaatct
    29701 gttagccatt ttgaggataa aaagatgcgt cttcttcata ccatgctgcg cgttggcgat
    29761 ttgcaacgct ccatcgattt ttataccaaa gtgctgggca tgaaactgct gcgtaccagc
    29821 gaaaacccgg aatacaaata ctcactggcg tttgttggct acggcccgga aaccgaagaa
    29881 gcggtgattg aactgaccta caactggggc gtggataaat acgaactcgg cactgcttat
    29941 ggtcacatcg cgcttagcgt agataacgcc gctgaagcgt gcgaaaaaat ccgtcaaaac
    30001 gggggtaacg tgacccgtga agcgggtccg gtaaaaggcg gtactacggt tatcgcgttt
    30061 atggaagatc cggacggtta caaaattgag ttaatcgaag agaaagacgc cggtcgcggt
    30121 ctgggcaact aatctcctgc cgggcgtgaa ctcatcgcgc ccgcatcttt actgcatcga
    30181 caagtaatat ttgtcataat gcgcgctgca atttatccgt attaagagaa tcagatgtcc
    30241 gataacgctc aacttaccgg tctgtgcgac cgttttcgtg gtttttatcc tgttgtgatc
    30301 gatgttgaaa cagccggatt taacgccaaa accgatgcgc tgcttgagat tgccgccatc
    30361 accctgaaaa tggatgaaca aggctggctg atgccggaca ccacattaca tttccacgtc
    30421 gaaccatttg tcggcgcaaa tttgcaacca gaagccctcg ccttcaacgg cattgacccg
    30481 aacgatcccg atcgtggcgc ggtcagcgaa tacgaggcgc tgcacgaaat ttttaaagtt
    30541 gtacgtaaag gtattaaagc gagcggctgt aaccgcgcca ttatggtggc gcacaatgcc
    30601 aattttgatc acagctttat gatagccgcc gcagaacgcg cctcactgaa acgtaacccg
    30661 ttccaccctt tcgccacttt tgacactgct gcactggccg ggctggcact cggacaaacc
    30721 gtattgtcaa aggcttgcca gaccgctggc atggacttcg acagcaccca ggcgcactcc
    30781 gcgctgtacg acaccgaacg cactgctgtg ctgttttgtg aaatcgtcaa ccgctggaaa
    30841 cgtctgggag gctggccgct acctgccgcc gaagaggtgt aatcgagtcg atgcctgatg
    30901 acatgcaatg attcaggcat ctatagtgag gctattccac gcatcctgca tgatattcac
    30961 ggggaatagc gttaatggca gataatccag acccttcatc gctcctgccg gacgtgtttt
    31021 caccggcgac ccgcgactgg tttcttcgcg cctttaaaca gccgaccgct gtccagtcgc
    31081 aaacctggca tgtggcggcg cgaagcgaac atgcgctggt gattgcaccg accggctccg
    31141 ggaaaacgct ggcagcattt ctctacgccc tcgatcggct cttccgcgaa ggcggcgaag
    31201 atacccgcga ggcgcataag cgtaaaacct cacgcatcct ctatatttca ccgataaaag
    31261 ctctgggcac cgacgttcag cgcaacttgc agctcccgtt gaagggtatt gccgatgaac
    31321 ggcggcggcg cggcgaaacg gtaattattg atgaagtcca cgcggtagcg ggcagtaaac
    31381 gtggtgcgca tctggcgtta agtctggagc ggctcgatgc gctgctccac acctcagcac
    31441 agcgaattgg cctttctgcc actgtgcgct cagccagcga tgtggcagcc tttcttggtg
    31501 gcgatcgccc ggttactgta gtcaacccgc ccgcaatgcg ccatccgcag atacgaattg
    31561 tcgtaccggt cgccaatatg gatgatgtct catcggtcgc cagcagcacc ggcgaagaca
    31621 gccatgccgg ccgggaaggc tccatctggc catatattga aacgggtatc cttgatgaag
    31681 tgttgcgcca tcgctcgacc attgtcttta ctaattcacg tgggctggcg gaaaaactga
    31741 cggcacgatt aaatgagctt tacgccgcac gcttacagcg ttccccgtct atcgccgttg
    31801 atgcggccca tttcgagtcg acctccggcg caacctctaa ccgtgtacaa agtagcgacg
    31861 tttttattgc ccgctcacac cacggctccg tctctaaaga acaacgagca atcaccgagc
    31921 aggcgctgaa atcgggtgag ttacgctgcg tggtcgcaac ctccagtctc gaactgggga
    31981 ttgatatggg cgcagtggat ctggtgattc aggtggcaac gccgctttct gttgccagtg
    32041 ggttacaacg cattggtcgc gccggacatc aggttggcgg tgtatctaaa gggctgtttt
    32101 tccctcgtac ccggcgtgac ttagtcgatt ccgcagtcat tgtagagtgt atgttcgcag
    32161 gcaggctgga aaacctgaca ccgccgcata atccactcga cgtccttgcg cagcaaaccg
    32221 ttgccgccgc ggcgatggag gcattacagg tagacgaatg gtactcccgc gtacgccgtg
    32281 ccgcaccgtg gaaagatctg ccaagacgtg tttttgacgc cacgctggat atgctttccg
    32341 ggcgctatcc ctctggcgat ttttctgctt ttcgccccaa actggtctgg aacagggaga
    32401 ccgggatatt gaccgtccga cctggcgctc aattgttggc ggttaccagc ggcggcacca
    32461 ttccggatcg tggcatgtat agcgtgttat tacccgaagg tgaagaaaag gccggttcgc
    32521 ggcgggtggg tgaactggat gaggagatgg tatatgagtc gcgggtgaac gacattatca
    32581 ctctcggcgc tacctcatgg cggatccagc aaatcacccg cgatcaggtg attgtgactc
    32641 ctgctccggg tcgttctgcc cggctcccct tctggcgtgg tgaaggtaac ggacgtccgg
    32701 ctgaattagg cgagatgatc ggcgattttc ttcatttact ggcggatggc gcgttctttt
    32761 ccgggactat tcccccgtgg ctggcagaag aaaatacgaa cgccaatatt caggggttga
    32821 ttgacgagca acgcaacgcg acgggcatcg ttccggggag tcgccatctg gtcctcgaac
    32881 ggtgccgtga tgaaattggc gactggcgta ttattttgca ctctccctat ggaagacggg
    32941 tgcatgaacc ctgggcgctg gcgattgccg ggcgaataca tgccctatgg ggcgctgacg
    33001 cgtctgtggt cgccagtgat gacggcattg ttgcgcgcat tcctgacact gatggtaaat
    33061 tgcccgacgc cgcgattttt ttgtttgaac cagaaaagtt gctgcaaatt gtccgcgagg
    33121 cggtaggcag ctcggcactt ttcgccgccc gttttcgcga atgcgccgcg cgggcattat
    33181 taatgccggg gcgcactccg ggccatcgca ccccgctttg gcaacaacgg ctgcgcgcca
    33241 gtcagttgct ggaaatcgct cagggatatc cagattttcc ggtcattctc gaaatcctac
    33301 gcgaatgtct gcaagatgtt tatgatcttc ccgcactgga acgtttgatg cgtcgcctga
    33361 acggtggcga aattcaaata tccgatgtaa cgaccactac gccctcgcct ttcgccacaa
    33421 gtttattgtt cggctatgtc gcggaattta tgtaccagag cgacgccccg ctggcagagc
    33481 gccgggcatc cgtactgtcg ctggacagcg agttactgcg caatctactc ggacaggtcg
    33541 atccggggga attactcgac ccgcaggtca ttcgccaggt ggaagaagag ttgcaacgac
    33601 tggctcctgg cagaagagcg aaaggtgaag aaggattgtt cgacctgctg cgcgaactgg
    33661 ggccaatgac cgttgaagac ctggcgcaac ggcatacagg cagcagtgaa gaggttgcgt
    33721 cgtatctgga aaatcttctt gcagtaaaac gaatcttccc agcgatgatt agcggacagg
    33781 agcgtcttgc ctgtatggat gatgccgcca ggctgcgtga tgccctcggc gtacgactac
    33841 cagagtcatt gccagagatt tatttacata gagtcagtta cccgcttcgc gacctctttc
    33901 tgcgctatct ccgggctcat gctctggtca cggctgaaca actggctcat gagtttagtc
    33961 tcggtattgc cattgtcgaa gagcagcttc agcaactgcg tgaacagggt ctggtgatga
    34021 atctgcaaca agacatctgg gtgagcgatg aagtatttcg tcgtctgcgt ttgcgctcgc
    34081 tgcaagccgc cagagaagcg acgcgtcccg ttgcagccac gacctatgcg cgattgctgc
    34141 tggaacgtca gggcgtatta cccgccaccg atggtagccc ggcgctcttt gcctcaacat
    34201 cgccaggcgt ttatgagggc gtagatggcg tgatgcgggt gatcgaacag cttgccggag
    34261 tcggtttacc cgcctcactc tgggaaagcc agatcctgcc tgcccgcgta cgcgactatt
    34321 cgtcagaaat gctcgatgaa ttactggcaa ccggtgcggt tatctggtcg gggcaaaaaa
    34381 aactgggtga agatgacggc ctggtggcac tgcatctaca ggcatatgct gcagaatcgt
    34441 tcactcccgc cgaagcggat caggcgaatc gttcggcgct gcaacaagcg atagtcgctg
    34501 ttctggctga cggaggagcc tggtttgcac aacaaatcag ccaacggata cgcgacaaaa
    34561 tcggcgaatc ggttgatctc tctgccctgc aagaggcgtt atgggcgctg gtctggcaag
    34621 gcgtcatcac cagcgacatt tgggcaccgt tacgcgccct cacccgcagc agttccaacg
    34681 cacgcacctc aactcgccgc agtcaccggg ctcgtcgtgg acgtcctgtc tatgcgcaac
    34741 ccatctcgcc gctgg
//
LOCUS       DAJVUJ010000024        33949 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.024, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000024 DAJVUJ010000000
VERSION     DAJVUJ010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33949
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.024"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(<1..318)
                     /locus_tag="OQQ48_001088"
     CDS             complement(<1..318)
                     /locus_tag="OQQ48_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1951934.1"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELGLSRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAREIRDQGYRGGMTILRAFIR
                     SLSV"
     gene            1672..2451
                     /gene="ipaJ"
                     /locus_tag="OQQ48_001089"
     CDS             1672..2451
                     /gene="ipaJ"
                     /locus_tag="OQQ48_001089"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     protease IpaJ"
                     /protein_id="HCS1951935.1"
                     /translation="MSEQRKPCKRGCIHTGVMLYGVLLQGAIPREYMISHQTDVRVNE
                     NRVNEQGCFLARKQMYDNSCGAASLLCAAKELGVDKIPQYKGSMSEMTRKSSLDLDNR
                     CERDLYLITSGNYNPRIHKDNIADAGYSMPDKIVMATRLLGLNAYVVEESNIFSQVIS
                     FIYPDARDLLIGMGCNIVHQRDVLSSNQRVLEAVAVSFIGVPVGLHWVLCRPDGSYMD
                     PAVGENYSCFSTMELGARRSNSNFIGYTKIGISIVITNEAL"
     gene            complement(3396..4325)
                     /gene="virB"
                     /locus_tag="OQQ48_001090"
     CDS             complement(3396..4325)
                     /gene="virB"
                     /locus_tag="OQQ48_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence regulon transcriptional activator
                     VirB"
                     /protein_id="HCS1951936.1"
                     /translation="MVDLCNDLLSIKEGQKKEFTLHSGNKVSFIKAKIPHKRIQDLTF
                     VNQKTNVRDQESLTEESLADIIKTIKLQQFFPVIGREIDGRIEILDGTRRRASAIYAG
                     ADLEVLYSKEYISTLDARKLANDIQTAKEHSIRELGIGLNFLKVSGMSYKDIAKKENL
                     SRAKVTRAFQAASVPQEIISLFPIASELNFNDYKILFNYYKGLEKANESLSSTLPILK
                     EEIKDLDTNLPPDIYKKEILNIIKKSKNRKQNPSLKVDSLFISKDKRTYIKRKENKTN
                     RTLIFTLSKINKTVQREIDEAIRDIISRHLSSS"
     gene            complement(4542..4778)
                     /locus_tag="OQQ48_001091"
     CDS             complement(4542..4778)
                     /locus_tag="OQQ48_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCS1951937.1"
                     /translation="MIKEKILSIVAFCYGIAYSKLSEETKFIEDLSADSLSLIEMLDM
                     ISFEFNLRIDESALEHIITIGDLISVVKNSTKSI"
     gene            complement(4782..6683)
                     /gene="ipaA"
                     /locus_tag="OQQ48_001092"
     CDS             complement(4782..6683)
                     /gene="ipaA"
                     /locus_tag="OQQ48_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector invasin IpaA"
                     /protein_id="HCS1951938.1"
                     /translation="MHNVNNTQAPTFLYKATSPSSTEYSELKSKISDIHSSQTSLKTP
                     ASVSEKENFATSFNQKCLDFLFSSSGKEDVLRSIYSNSMNAYAKSEILEFSNVLYSLV
                     HQNDLNFENEKGLQKIVAQYSELIIKDKLSQDSAFGPWSAKNKKLHQLRQNIEHRLAL
                     LAQQHTSGEALSLGQKLLNTEVSSFIKNNILAELKLSNETVSSLKLDDLVDAQAKLAF
                     DSLRNQRKNTIDSKGFGIGKLSRDLNTVAVFPELLRKVLNDILEDIKDSHPIQDGLPT
                     PPEDMPDGGPTPGANEKTSQPVIHYHINNDNRTYDNRVFDNRVYDNSYHENPENDAQS
                     PTSQTNDLLSRNGNSLLNPQRALVQKVTSVLPHSISDTVQTFANNSALEKVFNHTPDN
                     SDGIGSDLLTTSSQERSTNNSLSRGHRPLNIQNSSTTPPLHPEGVTSSNDNSSDTTKS
                     SASLSHRVASQINKFNSNTDSKVLQTDFLSRNGDTYLTRETIFEASKKVTNSLSNLIS
                     LIGTKSGTQERELQEKSKDITKSTTEHRINNKLKVTDANTINYVTETNADTIDKNHAI
                     YEKAKEVSSALSKVLSKIDDTSAELLTDDISDLKNNNDITAENNNIYKAAKDVTTSLS
                     KVLKNINKD"
     gene            complement(6692..7690)
                     /gene="ipaD"
                     /locus_tag="OQQ48_001093"
     CDS             complement(6692..7690)
                     /gene="ipaD"
                     /locus_tag="OQQ48_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle tip complex
                     protein IpaD"
                     /protein_id="HCS1951939.1"
                     /translation="MNITTLTNSISTSSFSPNNTNGSSTETVNSDIKTTTSSHPVSSL
                     TMLNDTLHNIRTTNQALKKDLSQKTLTKTSLEEIALHSSQISMDVNKSAQLLDILSKK
                     EYPINKDARELLHSAPKEAELDGYEMISHRELWDKIAKSINNINEQYLKVYEHAVSSY
                     TQMYQDFSAVLSSLAGWISPGGNDGNSVKLQVKSLKDELTKLKEKYKDKPLYPANNTV
                     SKEQANKWLTELGGTIGKVSEKNGGYVVNINMTPIDNMLKSLDNLGGNGEVVLDNAKY
                     QAWNAGFSAEDETMKNNLQTLVQKYSNANSIFDNLVKVLSSTISSCTDTDKLFLHF"
     gene            complement(7741..8832)
                     /gene="ipaC"
                     /locus_tag="OQQ48_001094"
     CDS             complement(7741..8832)
                     /gene="ipaC"
                     /locus_tag="OQQ48_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS translocon subunit IpaC"
                     /protein_id="HCS1951940.1"
                     /translation="MEIQNTKSAPILYTDISTKQTQSSSETQKSQNYQQLAAHIPLNV
                     GKNPVLTTTLNDDQLLKLSEQVQHDSEIIARLTDKKMKDLSEMSHTITPENTLDISSL
                     SSNAVSLIISVAVLLSALRTAETRLGSQLSLIAFDATKSAAENIVRQGLAALSSSITG
                     AVTQVGITGIGAKKTHSGISEQKGALRKNLATAQSLEKELAGSKLGLNKQIDTNITSP
                     QTNSSTKILGKNKLAPDNISLSTEHKTSLSSPDISLQDKIDTQRRAYELNTLSAQQKQ
                     NIGRATMETSAVAGNISTSGGRYTSALEEEEQLISQASSKQAEEASQVSKEASQATNQ
                     LIQKLLNIIDNINQSRNSTASQIAGNIRA"
     gene            complement(8852..10594)
                     /gene="ipaB"
                     /locus_tag="OQQ48_001095"
     CDS             complement(8852..10594)
                     /gene="ipaB"
                     /locus_tag="OQQ48_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     IpaB"
                     /protein_id="HCS1951941.1"
                     /translation="MHNVSTTTTGLPLAKILASTELGDNTIQAANDAANKLFSLTIAD
                     LAANKNINTTNAHSTSNILIPELKAPKSLNASSQLTLLIGNLIQILGEKSLTALTNKI
                     TAWKSQQQARQQKNLEFSDKINTLLSETEGLTRDYEKQINKLKNADSKIKDLENKINQ
                     IQTRLSELAPDSPENKKLRREEIQLTIKKDAAVKDRTLIEQKTLSIHSKLTDKSMQLE
                     KEIDSFSAFSNTASAEQLSTQQKSLTGLASVTQLMATFIQLVGKNNEESLKNDLALFQ
                     SLQESRKTEMERKSDEYAAEVRKAEELNRVMGCVGKILGALLTIVSVVAAAFSGGASL
                     ALAAVGLALMVTDAIVQAATGNSFMEQALNPIMKAVIEPLIKLLSDAFTKMLEGLGVD
                     SKKAKMIGSILGAIAGALVLVAAVVLVATVGKQAAAKLAENIGKIIGKTLTDLIPKFL
                     KNFSSQLDDLITNAVARLNKFLGAAGDEVISKQIISTHLNQAVLLGESVNSATQAGGN
                     VASAVFQNNASKNLADLTLSKYQVEQLSKYIGDAIEKFGQLQEVIAELLASMSNSQAN
                     RTDVAKAILQQTTA"
     gene            complement(10600..11067)
                     /gene="ipgC"
                     /locus_tag="OQQ48_001096"
     CDS             complement(10600..11067)
                     /gene="ipgC"
                     /locus_tag="OQQ48_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone IpgC"
                     /protein_id="HCS1951942.1"
                     /translation="MSLNITENESISTAVIDAINSGATLKDINAIPDDMMDDIYSYAY
                     DFYNKGRIEEAEVFFRFLCIYDFYNVDYIMGLAAIYQIKEQFQQAADLYAVAFALGKN
                     DYTPVFHTGQCQLRLKAPLKAKECFELVIQHSNDEKLKIKAQSYLDAIQDIKE"
     gene            complement(11125..11751)
                     /gene="ipgB1"
                     /locus_tag="OQQ48_001097"
     CDS             complement(11125..11751)
                     /gene="ipgB1"
                     /locus_tag="OQQ48_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB1"
                     /protein_id="HCS1951943.1"
                     /translation="MQILNKILPQVEFAIPRPSFDSLSHNKLVKKILSVFNLKQRFPQ
                     KNFGCPVNINKIRDSVIDKIKDSNSGNQLFCWMSQERTSYVSSMINRSIDEMAIHNGV
                     VLTSDNKKNIFAAIEKKFPDIKLDEKSAQTSISHTALNEIASSGLRAKILKRYSSDMD
                     LFNTQMKDLTNLVSSSVYDKIFNESTKVLQIEISAEVLKAVYRQSNTN"
     gene            complement(11767..12156)
                     /gene="ipgA"
                     /locus_tag="OQQ48_001098"
     CDS             complement(11767..12156)
                     /gene="ipgA"
                     /locus_tag="OQQ48_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgA"
                     /protein_id="HCS1951944.1"
                     /translation="MCRKLYDKLYEITGAKLDFNDKNQAFILLEEQIPVCITDNDEYI
                     FLTGLLNEHELFTENIINPEHILILNYSLSRDYGSSICLLPDTHQCVLTKKHYKKYLS
                     PDELIESLYEFLFCIKLTIANITSEVN"
     gene            complement(12169..13653)
                     /gene="icsB"
                     /locus_tag="OQQ48_001099"
     CDS             complement(12169..13653)
                     /gene="icsB"
                     /locus_tag="OQQ48_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector IcsB"
                     /protein_id="HCS1951945.1"
                     /translation="MILKISNFIDASNTKGPIRVEDTEHGPILVAQKFNLKDLFFRTL
                     STINAKINSQILNEQLKNYRLANQKSLLLFLKTLASEKSAESAFAAYEAVKNSIQHSF
                     TGKDIKLMLNTAESFHGIGTAKNLERHLVFRCWGNRGITHLGHTSISIKNNLLQEPTH
                     TYLSWYPGGNVTKDTEINYLFEKRSGYSVDTYKQDKLNMISDQTAERLDAGQEVRNLL
                     NSKQDQNNNKKIFFPRANQKKDPYGYWGVSADKVYIPLSGDNKTKDGKISYNLFGLDE
                     TNMSKFICQKKADAFRQLANYKLISKSENCAGMALNVLKAGNSEIYFPLPDVKLVATP
                     NNVYAYANKVRQRIESLNQSYNEIMKYIESDFDLSRLTQLRRSYLKSFNKINLIHTPK
                     TFKPLSISLYKHPTENVSSEDFDAVINACHSYLVKSAPSNMSRVLNELKTGATDKKEV
                     IIEKSIKTIDYYNSLKSPDLGTKLYIHDLLQVNKLLLNNSHSNI"
     gene            13967..15583
                     /locus_tag="OQQ48_001100"
     CDS             13967..15583
                     /locus_tag="OQQ48_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector inositol
                     phosphate phosphatase"
                     /protein_id="HCS1951946.1"
                     /translation="MHITNLGLHQVSFQSGDSYKGAEETGNQKGVSVISYQRVKNGER
                     NKGVEALNRLYLQNQTSLTGKGLLFARDRAAVFYEAIKLAGGDTSKIKAMMEQLDTYK
                     LGEVDKRNINELNKVISEEIRAQLGIKNKKELQSEIKQIFTNYLNNKNWEPINKNINY
                     NGKNYGFQLTPASHMKIGNKNIFVKEYNGKGICCASTRESKHIANMWLSKVVDDEGKE
                     IFSGIRHGVISAYGLKKNSSERAVAARNKAEELASAALYSRQELLSQALSGKTVDLKI
                     VSTSLLTPTSLTGGEESMLKDQVKALKELNSKRGEPTKLLIRNSDGLLQEVKVNLKVV
                     TFNFGVNELALKMGLGWRNVDKLNGESICSLLGDNFLKNGVIGGWAAEAIEKNPPCKN
                     DVIYLANQIKEILNKKLQKNDNGEPYKLSQRMALLAYTIGAVPCWNCKSGKDRTGMQD
                     AEIKREIIRKHETGQFSQLNSKLSSEEKRLFSTILMNSGNMEIQEMNTGVPGNKVMKK
                     LPLSSLELSYAERIGDPKIWNMVKGYSSFV"
     gene            15593..15955
                     /gene="ipgE"
                     /locus_tag="OQQ48_001101"
     CDS             15593..15955
                     /gene="ipgE"
                     /locus_tag="OQQ48_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgE"
                     /protein_id="HCS1951947.1"
                     /translation="MEDLADVICRALGIPSIDTDDQAIMLDDDVLIYIEKEGDSINLL
                     CPFCALPENINDLIYALSLNYSEKICLATDDEGGSLIARLDLTGINEFEDIYVNTEYY
                     ISRVRWLKDEFARRMKGY"
     gene            15955..16413
                     /gene="ipgF"
                     /locus_tag="OQQ48_001102"
     CDS             15955..16413
                     /gene="ipgF"
                     /locus_tag="OQQ48_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system lytic transglycosylase
                     IpgF"
                     /protein_id="HCS1951948.1"
                     /translation="MSRFVFIFLCFIPYLGRADCWDKAGERYNIPSSLLKAIAEKESG
                     FNKFAVNVNNNGSKDYGIMQINDFHSKRLREMGYTEEMLISHPCLSVHYAAKLLNEFM
                     LMYGRGWEAVGAYNAGTSPKKKKERLKYAEDIYKRYLRIAAESKQNNRRI"
     gene            16422..17537
                     /gene="mxiG"
                     /locus_tag="OQQ48_001103"
     CDS             16422..17537
                     /gene="mxiG"
                     /locus_tag="OQQ48_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiG"
                     /protein_id="HCS1951949.1"
                     /translation="MSEAKNSNLAPFRLLVKLTNGVGDEFPLYYGNNLIVLGRTIETL
                     EFGNDNFPENIIPVTDSKSDGIIYLAISKDNICQFSDEKGEQIDINSQFNSFEYDGVS
                     FQLKNMMEDKSRVHILNGMYNKPSVFFFFAVIVVLIIIFSLSLKKDEVKEIAEIIDDK
                     RYGIVNTGQCNYILAETQNDAVWASVALNKSGFTKCRYILVSNKEINRIQQYINQRFP
                     YINLYVLNLVSDKAELLVFLSKERNSSKDTELEKLKNALIVEFPYIKNIKFNYLSDHN
                     AREDAKGIFTKVNVQYKEICETNKVTYSVREELTDEKLELINRLISDHKNVYGDQYIE
                     FSVLLIDDDFKGKSYLNSKDSYVMLNDKHWFFLDKNK"
     gene            17545..17796
                     /locus_tag="OQQ48_001104"
     CDS             17545..17796
                     /locus_tag="OQQ48_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscF/YscF/HrpA family type III secretion system
                     needle major subunit"
                     /protein_id="HCS1951950.1"
                     /translation="MSVTVPDKDWTLSSLSETFDDGTQTLQGQLTSALNALAENPSNP
                     QLLAEYQSKLSEYTLYRNAQSNTVKVIKDVDAAIIQNFR"
     gene            17809..18102
                     /gene="mxiI"
                     /locus_tag="OQQ48_001105"
     CDS             17809..18102
                     /gene="mxiI"
                     /locus_tag="OQQ48_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod protein
                     MxiI"
                     /protein_id="HCS1951951.1"
                     /translation="MNYIYPVNQVDIIKASDFQSQEISSLEDVVSAKYSDIKMDTDIQ
                     VSQIMEMVSHPESLNPESLAKLQTTLSNYSIGVSLAGTLARKTVSAVETLLKS"
     gene            18108..18833
                     /gene="mxiJ"
                     /locus_tag="OQQ48_001106"
     CDS             18108..18833
                     /gene="mxiJ"
                     /locus_tag="OQQ48_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctJ family type III secretion inner membrane
                     ring lipoprotein Mxi"
                     /protein_id="HCS1951952.1"
                     /translation="MIRYKCFILFLLLMLIGCEQREELISNLSQRQANEIISVLERHN
                     ITARKVDGGKQGISVQVEKGTFASAVDLMRMYDLPNPERVDISQMFPTDSLVSSPRAE
                     KARLYSAIEQRLEQSLVSIGGVISAKIHVSYDLEEKNISSKPMHISVIAIYDSPKESE
                     LLVSNIKRFLKNTFSDVKYENISVILTPKEEYVYTNVQPVKEIKSEFLTNEVIYVFLG
                     MAVLVVILLVWAFKTGGFKRNKI"
     gene            18830..19357
                     /locus_tag="OQQ48_001107"
     CDS             18830..19357
                     /locus_tag="OQQ48_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HCS1951953.1"
                     /translation="MIRMDGIYKKYLSIIFDPAFYINRNRLNLPSELLENGVIRSEIN
                     NLIINKYDLNCDIEPLSGVTAMFVANWNLLPAVAYFIGSQESRLINHSEMVISYYGGK
                     ISKQGEAAIRSGFWHLIEWKENISVGIYERINLLFNPIALEGNYTPVERNLSRLNEGM
                     QYAKRHFTGIQTSCL"
     gene            19299..19994
                     /gene="mxiN"
                     /locus_tag="OQQ48_001108"
     CDS             19299..19994
                     /gene="mxiN"
                     /locus_tag="OQQ48_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mxi-Spa type III secretion system linker protein
                     MxiN"
                     /protein_id="HCS1951954.1"
                     /translation="MKVCNMQKGTLPVSRHHAYDGVVIKRIEKELCKTIKDRDTESKK
                     KAICVIKDATKKAESLRIDAVCDGYQIGIQTAFEHIIDYICEWKLKQNENRRNIEDYI
                     TSLLSENLHDERIISTLLEQWLSSLRNTVTELKVVLPKCNLALRKKLELDLHKYRSDV
                     KIILKYSEGNNYIFCSGNQVVEFSPQDVISGVKIELAEKLTKNDKKYFKELAHKKLRQ
                     IAEDLLKENPVND"
     gene            19987..20394
                     /gene="mxiM"
                     /locus_tag="OQQ48_001109"
     CDS             19987..20394
                     /gene="mxiM"
                     /locus_tag="OQQ48_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiM"
                     /protein_id="HCS1951955.1"
                     /translation="MINQINASNALQQRLNSEEFVNLNERLSSSQSFDENIIYEIMQY
                     FSQSELNSIDNDELHNKIEQLFNSRFPYLTAAQKSSLLNKLIDANQYVDLHEGFYASL
                     SIYNNIDFYIKTTTFDSLISVFEAGREADDSTW"
     gene            20378..20806
                     /gene="mxiM"
                     /locus_tag="OQQ48_001110"
     CDS             20378..20806
                     /gene="mxiM"
                     /locus_tag="OQQ48_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system pilotin MxiM"
                     /protein_id="HCS1951956.1"
                     /translation="MIRHGSNKLKIFILSILLLTLSGCALKSSSNSEKEWHIVPVSKD
                     YFSIPNDLLWSFNTTNKSINVYSKCISGKAVYSFNAGKFMGNFNVKEVDGCFMDAQKI
                     AIDKLFSMLKDGVVLKGNKINDTILIEKDGEVKLKLIRGI"
     gene            20936..21568
                     /gene="mxiE"
                     /locus_tag="OQQ48_001111"
     CDS             20936..21568
                     /gene="mxiE"
                     /locus_tag="OQQ48_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system transcriptional
                     activator MxiE"
                     /protein_id="HCS1951957.1"
                     /translation="MEGFFFVRNQNIKFSDNVNYHYRFNINSCAKFLAFWDYFSGALV
                     EHSHAEKCIHFYHENDLRDSCNTESMLDKLMLRFIFSSDQNVSNALAMIRMTESYHLV
                     LYLLRTIEKEKEVRIKSLTEHYGVSEAYFRSLCRKALGAKVKEQLNTWRLVNGLLDVF
                     LHNQTITSAAMNNGYASTSHFSNEIKTRLGFSARELSNITFLVKKINEKI"
     gene            21555..23255
                     /gene="mxiD"
                     /locus_tag="OQQ48_001112"
     CDS             21555..23255
                     /gene="mxiD"
                     /locus_tag="OQQ48_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system secretin MxiD"
                     /protein_id="HCS1951958.1"
                     /translation="MKKFNIKSLTLLIVLLPLIVNANNIDSHLLEQNDIAKYVAQSDT
                     VGSFFERFSALLNYPIVVSKQAAKKRISGEFDLSNPEEMLEKMTLLVGLIWYKDGNAL
                     YIYDSGELISKVILLENISLNYLIQYLKDANLYDHRYPIRGNISDKTFYISGPPALVE
                     LVANTATLLDKQVSSIGTDKVNFGVIKLKNTFVSDRTYNMRGEDIVIPGVATVVERLL
                     NNGKALSNRQAKNDPMPPFNITQKVSEDSNDFSFSSVTNSSILEDVSLIAYPETNSIL
                     VKGNDQQIQIIRDIITQLDVAKRHIELSLWIIDIDKSELNNLGVNWQGTASFGDSFGA
                     SFNMSSSASISTLDGNKFIASVMALNQKKKANVVSRPVILTQENIPAIFDNNRTFYVS
                     LVGERNSSLEHVTYGTLINVIPRFSSQGQIEMSLTIEDGTGNSQSNYNYNNENTSVLP
                     EVGRTKISTIARVPQGKSLLIGGYTHETNSNEIVSIPFLSSIPVIGNVFKYKTRNISN
                     IVRVFLIQPREIKESSYYNTAEYKSLISEREIQKTTQIIPSETTLLEDEKSLVSYLNY
                     "
     gene            23274..24341
                     /gene="mxiC"
                     /locus_tag="OQQ48_001113"
     CDS             23274..24341
                     /gene="mxiC"
                     /locus_tag="OQQ48_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HCS1951959.1"
                     /translation="MLDVKNTGVFSSAFIDKLNAMTNSDDGDETADAELDSGLANSKY
                     IDSSDEMASALSSFINRRDLEKLKGTNSDSQERILDGEEDEINHKIFDLKRTLKDNLP
                     LDQDFIDRLKRYFKDPSDQVLALRELLNEKDLTAEQVESLTKIINEIISGSEKSVNAG
                     INSAIQAKLFGNKMKLEPQLLRACYRGFIMGNISTTDQYIEWLGNFGFNHRHTIVNFV
                     EQSLIVDMDSEKPSCNAYEFGFVLSKLIAIKMIRTSDVIFMKKLESSSLLKDGSLSAE
                     QLLLTLLYIFQYPSESEQILTSVIDVSRASHEDSVVYQTYLSSVNESPHDIFKSESER
                     EIAINILRELVTSAYKKELSR"
     gene            24354..26414
                     /gene="mxiA"
                     /locus_tag="OQQ48_001114"
                     /gene_synonym="virH"
     CDS             24354..26414
                     /gene="mxiA"
                     /locus_tag="OQQ48_001114"
                     /gene_synonym="virH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein MxiA"
                     /protein_id="HCS1951960.1"
                     /translation="MIQSFLKQVSTKPELIILVLMVMIIAMLIIPLPTYLVDFLIGLN
                     IVLAILVFMGSFYIERILSFSTFPSVLLITTLFRLALSISTSRLILVDADAGKIITTF
                     GQFVIGDSLAVGFVIFSIVTVVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLK
                     AGIIDAAGAKERRSILERESQLYGSFDGAMKFIKGDAIAGIIIIFVNLIGGISVGMSQ
                     HGMSLSGALSTYTILTIGDGLVSQIPALLISISAGFIVTRVNGDSDNMGRNIMSQIFG
                     NPFVLIVTSALALAIGMLPGFPFFVFFLIAVTLTALFYYKKVVEKEKSLSESDSSGYT
                     GTFDIDNSHDSSLAMIENLDAISSETVPLILLFAENKISANDMEGLIERIRSQFFIDY
                     GVRLPTILYRTSNELKVDDIVLLINEVRADSFNIYFDKVCITDENGDIDALGIPVVST
                     SYNERVISWVDVSYTENLTNIDAKIKSAQDEFYHQLSQALLNNINEIFGIQETKNMLD
                     QFENRYPDLLKEVFRHVTIQRISEVLQRLLGENISVRNLKLIMESLALWAPREKDVIT
                     LVEHVRASLSRYICSKIAVSGEIKVVMLSGYIEDAIRKGIRQTSGGSFLNMDIEVSDE
                     VMETLAHALRELRNAKKNFVLLVSVDIRRFVKRLIDNRFKSILVISYAEIDEAYTINV
                     LKTI"
     gene            26427..26828
                     /gene="spa15"
                     /locus_tag="OQQ48_001115"
                     /gene_synonym="spaK"
     CDS             26427..26828
                     /gene="spa15"
                     /locus_tag="OQQ48_001115"
                     /gene_synonym="spaK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone Spa15/SpaK"
                     /protein_id="HCS1951961.1"
                     /translation="MSNINLVQLVRDSLFTIGCPPSIITDLDSHSAITISLDSMPAIN
                     IALVNEQVMLWANFDAPSDVKLQSSAYNILNLMLMNFSYSINELVELHRSDEYLQLRV
                     VIKDDYVHDGIVFAEILHEFYQRMEILNEVL"
     gene            26832..28124
                     /gene="spaL"
                     /locus_tag="OQQ48_001116"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
     CDS             26832..28124
                     /gene="spaL"
                     /locus_tag="OQQ48_001116"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SpaL"
                     /protein_id="HCS1951962.1"
                     /translation="MSYIKLLTQLSFPNRISGPILETSLSDVSIGEICNIQAGIESNE
                     IIARAQVVGFHDEKTILSLIGNSRGLSRQTLIKPTAQFLHTQVGRGLLGAVVNPLGEV
                     TDKFAVTDNSEILYRPVDNAPPLYSERAAIEKPFLTGVKVIDSLLTCGEGQRMGIFAS
                     AGCGKTFLMNMLIEHSGADIYVIGLIGERGREVTETVDYLKNSEKKSRCVLVYATSDY
                     SSVDRCNAAYIATAIAEFFRTEGHKVALFIDSLTRYARALRDVALAAGESPARRGYPV
                     SVFDSLPRLLERPGKLKAGGSITAFYTVLLEDDDFADPLAEEVRSILDGHIYLSRNLA
                     QKGQFPAIDSLKSISRVFTQVVDEKHRIMATAFRELLSEIEELRTIIDFGEYKPGENA
                     SQDKIYNKISVVESFLKQDYRLGFTYEQTMELLGETIR"
     gene            28217..28555
                     /gene="spa13"
                     /locus_tag="OQQ48_001117"
                     /gene_synonym="spaM"
     CDS             28217..28555
                     /gene="spa13"
                     /locus_tag="OQQ48_001117"
                     /gene_synonym="spaM"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858283.1"
                     /note="Not similar to SpaM from Salmonella T3SS; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein Spa13"
                     /protein_id="HCS1951963.1"
                     /translation="MEALDKRIIYFLQLENDLEPVGAQSVSQLFNTRRKIAIVKKHII
                     QYQSERILLKGRIEEIQKDIDEANASKRKLLHKESKICKRIGLIKRNNFAKQLILDEL
                     SQEDMKYGIR"
     gene            28542..29420
                     /gene="spa32"
                     /locus_tag="OQQ48_001118"
     CDS             28542..29420
                     /gene="spa32"
                     /locus_tag="OQQ48_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     regulator Spa32"
                     /protein_id="HCS1951964.1"
                     /translation="MALDNINLNFSSDKQIEKCEKLSSIDNIDSLVLKKKRKVEIPEY
                     SLIASNYFTIDKHFEHKHDKGEIYSGIKNAFELRNERATYSDIPESMAIKENILIPDQ
                     DIKAREKINIGDMRGIFSYNKSGNADKNFERSHTSSVNPDNLLESDNRNGQIGLKNHS
                     LSIDKNIADIISLLNGSVAKSFELPVMNKNTADITPSMSLQEKSIVENDKNVFQKNSE
                     MTYHFKQWGAGHSVSISVESGSFVLKPSDQFVGNKLDLILKQDAEGNYRFDSSQHNKG
                     NKNNSTGYNEQSEEEC"
     gene            29414..30295
                     /gene="spa33"
                     /locus_tag="OQQ48_001119"
                     /gene_synonym="spaO"
     CDS             29414..30295
                     /gene="spa33"
                     /locus_tag="OQQ48_001119"
                     /gene_synonym="spaO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctQ family type III secretion system ring
                     protein Spa33"
                     /protein_id="HCS1951965.1"
                     /translation="MLRIKHFDANEKLQILYAKQLCERFSIQTFKNKFTGSESLVTLT
                     SVCGDWVIRIDTLSFLKKKYEVFSGFSTQESLLHLSKCVFIESSSVFSIPELSDKITF
                     RITNEIQYATTGSHLCCFSSSLGIIYFDKMPVLRNQVSLDLLHHLLEFCLGSSNVRLA
                     TLKRIRTGDIIIVQKLYNLLLCNQVIIGDYIVNDNNEAKINLSESNGESDHTEVSLAL
                     FNYDDINVKVDFILLEKKMTINELKMYVENELFKFPDDIVKHVNIKVNGSLVGHGELV
                     SIEDGYGIEISSWMVKE"
     gene            30300..30950
                     /gene="spa24"
                     /locus_tag="OQQ48_001120"
                     /gene_synonym="spaP"
     CDS             30300..30950
                     /gene="spa24"
                     /locus_tag="OQQ48_001120"
                     /gene_synonym="spaP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa24/SpaP"
                     /protein_id="HCS1951966.1"
                     /translation="MLSDMSLIATLSFFTLLPFLVAAGTCYIKFSIVFVMVRNALGLQ
                     QVPSNMTLNGIALIMALFVMKPIIEAGYENYLNGPQKFDTISDIVRFSDSGLIEYKQY
                     LKKHTDLELARFFQRSEEENADLKSAENNDYSLFSLLPAYALSEIKDAFKIGFYLYLP
                     FIVVDLVISSILLALGMMMMSPITISVPIKLVLFVALDGWGILSKALIEQYINVPA"
     gene            30965..31225
                     /gene="spaQ"
                     /locus_tag="OQQ48_001121"
     CDS             30965..31225
                     /gene="spaQ"
                     /locus_tag="OQQ48_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaQ"
                     /protein_id="HCS1951967.1"
                     /translation="MSDIVYMGNKALYLILIFSLWPVGIATVIGLTIGLLQTVTQLQE
                     QTLPFGIKLIGVSISLLLLSGWYGEVLLSFCHEIMFLIKSGV"
     gene            31225..31995
                     /gene="spa29"
                     /locus_tag="OQQ48_001122"
                     /gene_synonym="spaR"
     CDS             31225..31995
                     /gene="spa29"
                     /locus_tag="OQQ48_001122"
                     /gene_synonym="spaR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa29/SpaR"
                     /protein_id="HCS1951968.1"
                     /translation="MDISSWFESIHVFLILLNGVFFRLAPLFFFLPFLNNGIISPSIR
                     IPVIFLVASGLITSGKVDIGSSVFEHVYFLMFKEIIVGLLLSFCLSLPFWIFHAVGSI
                     IDNQRGATLSSSIDPANGVDTSELAKFFNLFSAVVFLYSGGMVFILESIQLSYNICPL
                     FSQCSFRVSNILTFLTLLASQAVILASPVMIVLLLSEVLLGVLSRFAPQMNAFSVSLT
                     IKSLLAIFIIFICSSTIYFSKVQFFLGEHKFFTNLFVR"
     gene            32002..33030
                     /gene="spa40"
                     /locus_tag="OQQ48_001123"
                     /gene_synonym="spaS"
     CDS             32002..33030
                     /gene="spa40"
                     /locus_tag="OQQ48_001123"
                     /gene_synonym="spaS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     switch protein Spa40/SpaS"
                     /protein_id="HCS1951969.1"
                     /translation="MANKTEKPTPKKLKDAAKKGQSFKFKDLTTVVIILVGTFTIISF
                     FSLSDVMLLYRYVIINDFEINEGKYFFAVVIVFFKIIGFPLFFCVLSAVLPTLVQTKF
                     VLATKAIKIDFSVLNPVKGLKKIFSIKTIKEFFKSILLLIILALTTYFFWINDRKIIF
                     SQVFSSVDGLYLIWGGLFKDIILFFLAFSIFVIILDFVIEFILYMKDMMMDKQEIKRE
                     YIEQEGHFETKSRRRELHIEILSEQTKSDIRNSKLVVMNPTHIAIGIYFNPEIAPAPF
                     ISLIETNQCALAVRKYANEVGIPTVRDVKLARKLYKTHTKYSFVDFEHLDEVLRLIVW
                     LEQVENTH"
     gene            33043..33285
                     /locus_tag="OQQ48_001124"
     CDS             33043..33285
                     /locus_tag="OQQ48_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1951970.1"
                     /translation="MCYMGVNFCNKIGIDQSEFEIESSIINSIANEVLNPISFLSNKD
                     IINVLLRKISSECDLVRKDIYRCALELVVEKTPDDL"
BASE COUNT        10585 a         4963 c         6802 g        11599 t
ORIGIN      
        1 aaccgagaga gaacgaatga atgccctgag aatggtcatt ccgccacgat atccctggtc
       61 tctgatctcg cgagcgatta ccgttgccgg gattttgtaa ggatgagcat cggcgatgcg
      121 ttgacgaata taatcccggt attcatccag gagtgaagca acagcaggtc gcggcgtata
      181 ttttggcggc tcagattttg cctgcaaata acgtttaacg gtattgcggg agagccccag
      241 ttctctggca atcgcccggc tactcattcc ctgcttgtgc aggattttaa tttccataac
      301 tgtctcaaaa gtgaccataa gctctcctga atcaggagag cagattaccc cctggatctg
      361 atttcaggcg ttgggtgtgg atcactattg caccgttcgt gacacttgtt gactgcctgt
      421 gcggcatctg gtatcaaggt ggcgtgaagc cggttcaggt tctcatgcgg aacgttggaa
      481 cctatatccc cccgatacca aaggcgaaat gtaagtgaca aaatgcaatg aacacgttct
      541 ccgttaactg aggagcatgg tgataaataa aggtaaatgc agaaggtgta gagtctcgca
      601 gatttctgac atcatagagc ctaacattag atgcataacg ctacagattg tagaatctct
      661 acaggtgaaa tatacactgg ttggcgttta tgtcgcaaaa catgtcattc aggttcatct
      721 caccaataag gatatgagtg aagtggagga taagtgagtc tgctgtcaga gttttctggt
      781 gtatgtcagt aactgcagaa ccatgtctgc ttgatatggg agcttatgga ggctctctgt
      841 acaaggttag ggagctaaca aagcagggat aaaaagtccg cctgatgcag gcacggttcg
      901 ttaaatcttt tgccattggt aacaaaatgg tgcgatgaat gttcaggcta tctttatctt
      961 gatggtggtt cagctgtttg gtaaagaaat cgctgtaaca atagaagaac tgcaggcagt
     1021 actggtccca cattgtttgc tcagtcaact ggtgaagttc cgtagcgcgt aaattaatgc
     1081 tttgcatgaa acgttactgg agtggatgaa agaatacctg catatatcat tactgcgttg
     1141 gattaccagt acaacagatt gaatgaactg gacatgttga tagacggcat tgaaaagcag
     1201 cttataagca tagtgcggca gagttaattg gagagcactt cgccttctga gtattaagta
     1261 atcatggtgg tacctgcgga tactatctat tcactgtgct cggtcggcag ccttactgac
     1321 caaggagttg ggggagttga taacaaagtt gaaaaaacag ctctcaacca aggtggttat
     1381 tgtaacaata gcaaataagc tggtgtgacc tgtatcacaa taaccgatta tggacgtaaa
     1441 tacgacaaaa atatgtcagt atcaggcggt attaattgct gatgctgatg gacattaact
     1501 cttaataaaa gggtggatgc tgaaaggttg ttatggtgac ggacgtgatg acgacggaaa
     1561 agagtatgag gttttggatt taaagttcgt tcggggaata aggacccggt cagctaattc
     1621 tcatagtagt tcgcattgct ggatgcaata ctgtgctgca attactcgtc gatgtcggaa
     1681 caacggaagc cttgcaaacg gggatgtatt catacaggtg ttatgttata tggggtttta
     1741 ttgcaggggg ctataccaag agaatatatg atatctcatc agactgatgt gagggtaaat
     1801 gagaatagag taaacgaaca aggttgtttt ttggcgagga agcagatgta tgacaattct
     1861 tgtggagctg ccagtttact ctgtgctgca aaggaattag gggttgataa aataccacaa
     1921 tacaaagggt ctatgtccga aatgacaagg aagagttctc ttgatttgga taatcgttgt
     1981 gagcgggatt tatacttaat aactagtggt aattataatc cgagaataca caaagataat
     2041 atcgcggatg ccggttattc tatgcctgat aagatagtca tggctacgag gcttcttgga
     2101 ttgaatgctt atgtcgttga agaaagtaac attttttcgc aagtaattag ttttatttat
     2161 cccgatgcaa gggacttatt aattggcatg ggatgtaata tagtacatca acgagatgtg
     2221 ttatcttcta accaaagagt tttggaagct gttgctgtat cctttattgg agttcctgtc
     2281 ggcctccact gggtgttgtg tagacctgat ggaagctata tggatccagc tgtaggagaa
     2341 aattattctt gtttttcaac tatggaatta ggtgcaagaa gaagtaattc taactttata
     2401 ggatatacta aaataggtat ttccatagtt ataactaatg aggctttgta aattgtcgtt
     2461 ttttatctgt gaaggagctt gaatattttg gggcgggggg cataacaaat gatattactg
     2521 agtacaaatt tagctatatt gggcggatta gttgtgtggg aagacgaaat gtttctcttg
     2581 aaataagtga actccctcaa ggaatgattg tgtgcttaaa tatttaagtg ctggtaagtt
     2641 tgagaaggct gacatatttt gcagagcaaa cagtgattga cttggatgat atttttattt
     2701 tagtcactcg ttgtaattgg agtatgcttt attttatttg aaaatagatg tattttatac
     2761 ccgcaattta aattgtaagc cctatgaggg gtatggatat tataagttac tctttgacta
     2821 agcataactt ttggggttag aaaaataaat ctcttgttaa taattaatat gattttaaaa
     2881 tcaaacttgt ttataattat aaaataatgt aagtcggtca ttttatggga agattaggag
     2941 aataaaacag taattatatt tgagaaatct gctaatatat tcttaaagct tttttgatac
     3001 tgtgaacgaa gtctgttaat ggtggatttt ttgtttatca ccatgtaaaa aaattgagta
     3061 gtaaaagcgt tgactgctaa tcctcgctgt ttttattgaa acgattaaaa atacgctgaa
     3121 tattttcggc cagtcactca tcttcacata aaattttgac catcatgccg catcccttag
     3181 ttgcacaatt cattttcaat gatatgaata ttactaattt caccagataa tgttctcgtg
     3241 gtcagcaggg tatgagtttt caccgtaatt ttaaggaaac acagtgcgtg gggattgaat
     3301 aacagatagc cgacggaatt accccaccgg ctgaaagtca agttcaacga agatttttat
     3361 tatctgaatt gggcagttta catcagtgtt cgagtttatg aagacgatag atggcgagaa
     3421 attatatctc gaatagcttc atctatttct ctctgaactg ttttgtttat tttagataat
     3481 gtaaatatta gagttctgtt tgttttattc tcttttcttt ttatgtaagt ccgtttgtct
     3541 ttagaaataa ataacgagtc aacttttagc gaagggtttt gctttctgtt tttgcttttc
     3601 tttatgatgt ttaaaatttc ttttttatat atgtccgggg gcaaatttgt atcaaggtct
     3661 tttatttctt cctttaatat tggtagtgta gaactaagag attcattagc cttttcaagt
     3721 cctttataat aattgaataa tatcttgtag tcattaaagt taagttctga cgcgattgga
     3781 aatagagata ttatttcctg tggaacgctt gctgcctgaa aggcacgagt gactttcgcg
     3841 cgagacagat tctctttttt ggctatgtct ttataggaca tccctgatac tttcagaaaa
     3901 ttaagaccaa taccaagttc tcggatgcta tgctcttttg ctgtttgtat atcgtttgct
     3961 agttttctgg catcaagagt agatatatat tcttttgaat atagaacttc aagatctgct
     4021 cctgcatata ttgcagatgc tcttctacga gtgccatcca gaatttcaat tctaccatca
     4081 atctcccttc ctattacagg gaagaattgt tgtagcttta tagtttttat gatatcggct
     4141 aatgattctt ctgttaggga ttcttgatcg cgtacattcg ttttttggtt gacgaaggtt
     4201 aaatcttgga tccttttatg aggaatcttg gctttgataa aggaaacttt attaccagaa
     4261 tggagtgtaa actctttctt ttggccttcc tttatactta acaagtcgtt gcacaaatcc
     4321 accatatcac accctgttta ttcatattga tttatattga caaaagttaa atgcagtgtg
     4381 agcaagattg tatgtaactg ttgatgatag tcaacggtag tcttaaaaat tactatttta
     4441 atagctggat tgatgcaatt tatctttatt ggataaaaga ataatattct tgtggagctc
     4501 tgatgtgaga gctccataga tgaaaaaatg cttatcaatt actatattga ttttgtacta
     4561 tttttgacta cagaaataag atctccaata gtaataatgt gttctaatgc agattcatct
     4621 atacgtagat tgaactcaaa actaatcata tccagcattt caatcagaga aagagaatcg
     4681 gcagaaagat cttcaataaa tttagtttct tcagatagtt tgctgtacgc tataccatag
     4741 caaaaggcca ctattgataa tattttttct tttatcatat tttaatcctt attgatattc
     4801 tttaatactt ttgataggga agtggttaca tcttttgctg ctttatatat attattgttt
     4861 tcagctgtaa tatcattgtt attttttaaa tcagatatat catctgtaag taattctgca
     4921 gaggtatcgt caatttttga caataccttg ctgagggcgc tagatacttc ttttgccttt
     4981 tcatagatcg catgattttt atcaattgta tctgcgttgg tttctgttac gtagtttatt
     5041 gtatttgcat ctgtaacttt taatttgttg tttattctgt gttctgttgt ggattttgta
     5101 atgtcctttg atttttcctg taactctcgt tcttgtgttc ctgatttagt tccaatgaga
     5161 gatataagat tacttaggga gtttgttact ttttttgaag cttcaaatat cgtttcccgt
     5221 gttaaatatg tgtctccatt tcttgataaa aaatcagtct gaagtacttt tgaatcagtg
     5281 tttgagttga atttattgat ttgcgaagct actctatgag aaagagaagc gctactttta
     5341 gttgtatctg atgagttatc attactgctt gtcactcctt ccgggtggag agggggggtg
     5401 gttgaagagt tctgtatgtt cagaggcctg tgtccccgag aaagagagtt atttgtagat
     5461 ctttcttgac tactcgtagt taacaggtct gaacctattc catccgaatt atctggagta
     5521 tggttgaaaa ccttttctaa agctgaatta tttgcaaatg tctggacagt atctgatata
     5581 gagtgtggta gaacggaagt tactttttga actagtgctc tttgtggatt tagtaatgag
     5641 tttccgttac gggataatag atcgtttgtc tgagaagtag gagactgtgc atcattttct
     5701 gggttctcgt gatagctatt gtcatatact ctgttgtcaa aaactctatt atcgtaagtt
     5761 ctattatcat tatttatatg atagtgaatt acaggttggg atgttttctc attggctccg
     5821 ggggttggtc cgccatctgg catatcttcg ggaggtgtag ggaggccatc ttggatagga
     5881 tgcgaatctt ttatatcttc taaaatatca ttaaggactt ttctcaacag ctcaggaaac
     5941 acggctactg tatttaagtc tcttgacagt ttacctatac caaatccttt actatcaata
     6001 gtatttttac gttgattgcg caaactatca aaggcaagtt ttgcctgtgc gtcaactaaa
     6061 tcatctagtt tgagagatga aacagtttca ttacttaact ttaattcagc aagaatatta
     6121 ttcttgataa atgatgatac ttcagtattg aggagttttt gtcccaatga taaagcttca
     6181 ccagatgtgt gttgttgtgc taatagtgca agtctgtgct caatgttttg tcgtaattga
     6241 tggagtttct tattctttgc cgaccatggt ccaaaggcag aatcttggga taatttatct
     6301 tttataatta gttccgaata ctgtgcgaca attttttgaa gtcccttttc gttttcaaaa
     6361 ttaagatcat tttgatgtac taaggagtac aaaacatttg aaaattcgag aatctcgctt
     6421 ttggcatacg cattcattga gttggaataa atgcttctta acacatcttc tttccctgag
     6481 gaagaaaata aaaaatcaag acatttctga ttaaaagacg ttgcaaagtt ttctttttca
     6541 gacactgatg ctggtgtttt tagagaagtt tgcgaactat ggatatcgga tattttgctt
     6601 tttaactcgc tgtattctgt tgatgatggt gaagttgcct tatataagaa tgttggcgct
     6661 tgagtattat ttacattatg catggcgcac ctcagaaatg gagaaaaagt ttatctgtat
     6721 ctgtacatga gcttattgta ctactcaaaa cctttactaa attatcaaaa atactattgg
     6781 cattactgta tttttgaact aaagtttgaa gattattttt cattgtttca tcttcggcag
     6841 agaatccggc attccatgcc tgatattttg cattatctag cacaacctcg ccatttccac
     6901 ctagattatc taagcttttt aacatattgt ctattggggt catgtttata ttgacaacat
     6961 atcccccgtt tttttcagat accttgccga ttgttccacc taattctgta agccatttat
     7021 ttgcttgttc cttactaaca gtattatttg ctggatatag cggtttatct ttatattttt
     7081 ccttgagctt tgtcaattcg tctttaagcg atttgacttg taatttcacg gagtttccgt
     7141 cgttacctcc gggagagatc cagccggcaa gactggaaag aacagcgcta aaatcttgat
     7201 acatttgagt atatgaacta acggcatgtt catatacttt cagatactgt tcattaatat
     7261 tattgattga ctttgcaatt ttatcccaca gttctctgtg agatatcatt tcatatccat
     7321 caagctcggc ttctttcggg gctgaatgta ataattctct tgcgtcttta ttaattggat
     7381 attctttctt ggaaagaata tccaatagtt gagcggattt gtttacatcc atgctaatct
     7441 gagatgaatg taatgctatt tcttctagcg atgttttagt caacgttttt tgtgagagat
     7501 ctttctttaa tgcctgattt gttgttctga tattatgaag ggtgtcgttg agcatagtaa
     7561 gggaacttac aggatgagaa ctggtcgttg tttttatatc agaattaact gtttcggttg
     7621 atgaaccgtt ggtattgttt gggctgaatg atgaggtgga aatactatta gtcagagttg
     7681 ttatattcat ggttatttcc ttataggaag agccatatat tattatggct cttggatata
     7741 ttaagctcga atgttaccag caatctgact ggctgtagaa ttccttgatt ggttgatgtt
     7801 gtcaattata ttcaataatt tttgtattaa ttgatttgtc gcttgggatg cttctttaga
     7861 tacttgggat gcttcctctg cttgtttact gctggcctga ctgattagtt gttcttcttc
     7921 ttcaagagca gatgtataac gccctcctga tgtggatata ttaccagcaa cggctgatgt
     7981 ctccattgtt gcacggccaa tgttttgttt ttgctgagca gaaagggtat tgagctcgta
     8041 agctcttctc tgggtgtcaa ttttatcctg caaagaaata tcgggagaac taagagaagt
     8101 tttatgttca gttgacaggg atatattatc tggtgccagt ttatttttac ctaaaatttt
     8161 tgtgctagag ttagtttgtg gtgaggtgat atttgtatct atttgtttat ttaaccctaa
     8221 tttagaacct gcaagctctt tttcaagaga ttgagcagtg gcaaggttct ttcttaaggc
     8281 tcctttttgt tcgctaatcc ctgaatgcgt ttttttggca ccgatacccg ttatacctac
     8341 ttgtgtgacc gctccagtaa tgcttgatga tagggctgct aggccttgcc gaacaatgtt
     8401 ctctgcagct gattttgtag catcgaacgc aatcaatgac aattgagagc ccaatctagt
     8461 ttctgcagtg cggagagcag aaagtagaac ggctacacta ataattaaag aaacagcatt
     8521 agaagaaaga ctggaaatat ccagggtgtt ctctggggta attgtgtgac tcatttctga
     8581 aagatctttc atctttttgt cagtaaggcg agcaatgatt tctgaatcat gctgaacctg
     8641 ctctgataac tttaaaagtt gatcatcatt taatgtagtt gttaatacgg gatttttacc
     8701 gacattaagt ggaatatgtg ctgctaactg ctgataattt tgtgattttt gtgtttcgga
     8761 agaactttga gtttgttttg tagatatatc tgtatataaa atcggggctg attttgtgtt
     8821 ttgaatttcc ataacattct ccttatttgt atcaagcagt agtttgttgc aaaattgctt
     8881 ttgcaacatc agttctatta gcctgagagt tggacattga ggctaacagc tctgcaatta
     8941 cttcctgcaa ttggccgaat ttttctattg cgtcaccgat gtattttgac agttgttcaa
     9001 cttgatattt cgataatgtc aggtctgcta gatttttcga cgcgttgttt tggaaaacag
     9061 cagaagcgac atttcctccc gcttgtgtgg cagagttaac actttctcct aataaaactg
     9121 cttggtttaa atgggtggaa ataatttgtt tggatattac ttcatcaccc gctgcaccaa
     9181 gaaatttatt taatctggca acagcattag tgattaaatc gtccagttga gaagaaaaat
     9241 tcttgagaaa ctttggtata aggtctgtga gggttttacc tattattttg ccaatattct
     9301 ctgcaagttt tgctgctgcc tgtttaccaa cagtggctac gagaacgact gctgcaacca
     9361 agacaagagc gcctgcgatt gcccccagaa tagagccaat cattttggct tttttcgagt
     9421 cgacgcccaa gccttcgagc atttttgtaa atgcatctga aaggagtttg attaagggtt
     9481 caatgactgc tttcatgatc ggattcaggg cttgttccat gaaggaattg ccggtcgctg
     9541 cttgtactat agcatccgta accataagag ctaaaccaac agctgccagt gctagagatg
     9601 ctcctccaga aaaagctgct gcaacaacac taacgatagt taaaagtgcc ccaagtattt
     9661 tcccaacaca acccattact ctgttgagtt cttctgcttt acgtacttca gcagcatact
     9721 catcagattt tctctccatt tcagtttttc ttgattcttg gagagactgg aatagagcca
     9781 gatcattttt taaagattct tcattatttt ttccaactag ttgaataaag gttgccatca
     9841 attgagtaac actggcaagt ccggttaatg atttctgctg ggttgatagc tgttcagcag
     9901 atgctgtgtt tgaaaatgca gaaaaagagt ctatttcttt ttcgagttgc attgatttat
     9961 ctgtaagttt gctatgaatt gacagagttt tctgctcaat caatgtcctg tctttaactg
    10021 ctgcgtcttt tttgatagtg agttgtattt cttcccgtct taatttttta ttttctggtg
    10081 aatctggggc gagttcggat aatcttgttt gaatttggtt aattttattt tctaggtctt
    10141 ttattttaga atctgcgttt tttagtttat taatttgttt ttcatagtct ctggttagtc
    10201 cttcagtttc agatagaaga gtattaattt tatcggagaa ttctaggttt ttttgctgtc
    10261 ttgcctgttg ttgggacttc caggcagtaa ttttatttgt taatgcagtt aaagattttt
    10321 caccaagtat ttgaataagg tttccaatta aaagcgttag ttgggaactt gcatttaatg
    10381 actttggtgc tttaagttca gggattaata tattcgaagt tgagtgtgca ttagttgtat
    10441 taatattttt gttagcagca agatcagcaa ttgtaagaga aaataatttg ttagctgcat
    10501 catttgcagc ctggatagta ttgtctccaa gctcagtgga agccaatatt ttggcaagag
    10561 gcaaaccagt ggttgtggtg cttacattat gcataataat tactccttga tatcctgaat
    10621 tgcgtctaag tatgattgtg cttttatttt taatttttca tcattgctgt gttgaattac
    10681 gagttcgaag cactctttgg ctttcaaggg ggctttcaac cgaagctggc attgtccagt
    10741 atggaatacc ggtgtatagt cattttttcc taatgcaaaa gcgacagcat aaaggtctgc
    10801 tgcttgttgg aactgttctt ttatctgata aatagctgcg agtcccataa tgtagtctac
    10861 attgtaaaag tcgtatatac ataaaaatct gaagaaaact tcagcttcct ctattcttcc
    10921 tttgttgtaa aagtcataag catatgaata aatgtcatcc atcatatcat caggaattgc
    10981 attaatatct ttcagtgtag cgccagagtt aattgcatca attactgcag tagagatgct
    11041 ttcattttca gtgatattta aagacataag gtctcctttt tgtctgcatt actttaataa
    11101 gtataagatt taatataaac agatttaatt tgtattgctt tgacggtata cagcctttag
    11161 aacctctgcg cttatttcta tttgtaaaac ttttgttgat tcattaaata ttttatcgta
    11221 gacggaactg gatactagat ttgtaagatc cttcatttgt gtgttaaaca aatccatgtc
    11281 actgctataa cgttttaaaa tctttgctct gaggccagat gaggcaatct cgtttagtgc
    11341 tgtatgactg atagatgttt gtgctgattt ttcatcaagt tttatatccg ggaatttttt
    11401 ttcaatagct gcaaatatgt tttttttgtt atctgaggtc aaaacaactc cattgtgtat
    11461 ggccatttca tctatacttc tgtttatcat tgaagagaca taagaggttc gctcttggct
    11521 catccaacaa aatagttgat tccccgaatt actatctttt attttgtcta tgacgctgtc
    11581 cctgatttta tttatattca ccgggcatcc aaaattcttc tgaggaaagc gttgttttaa
    11641 gttaaaaaca gataatattt tctttacaag cttattgtga cttaacgaat caaatgatgg
    11701 gcgaggaatc gcgaattcaa cctgtggaag tattttgttt agaatttgca tgataccccc
    11761 tatatgttag ttcacttctg aagtgatgtt tgcaatggtt agtttaatac aaaataaaaa
    11821 ttcgtacaaa gattcaatga gttcatcagg tgaaagatat tttttgtagt gtttcttggt
    11881 caaaacacat tgatgagtat cagggagaag acagatagaa gaaccataat ctctactcaa
    11941 agagtagtta aggatgagta tgtgttctgg attaataata ttttctgtaa aaagttcatg
    12001 ttcattaaga agtccggtca aaaagatgta ttcatcatta tctgttatac aaacaggtat
    12061 ttgttcttcg agcaatataa aggcttgatt tttatcgtta aaatcaagct ttgcacctgt
    12121 tatttcataa agtttatcat atagtttgcg acacatttct atggccttct atatattaga
    12181 atgagagtta ttcaataaaa gcttattgac ctgtaataag tcgtgaatgt aaagtttggt
    12241 tccaagatct ggcgatttaa gagaattgta ataatcaata gtttttattg acttttcgat
    12301 aattacttct tttttatctg ttgctccggt ttttaattcg tttaatacac gagacatatt
    12361 gctgggtgct gattttacta agtatgagtg gcaagcgttg atgaccgcat caaagtcttc
    12421 tgaagataca ttttcagtag gatgtttgta aagtgaaatt gataaaggct tgaatgtttt
    12481 tggggtgtgg ataaggttaa ttttattgaa acttttaaga tagctacgtc gtaattgagt
    12541 caatctggaa agatcaaaat cagattcaat atacttcatt atctcgttat aagactgatt
    12601 gagactttca atgcgttgcc taactttatt tgcatacgcg tatacattgt taggtgtagc
    12661 aaccaattta acgtcaggga gtggaaagta tatttcagaa tttcctgctt taagaacatt
    12721 aagtgccata ccagcacagt tttcagattt actgattaat ttataatttg ccaattgtct
    12781 gaaagcatct gctttctttt ggcatataaa ttttgacatg tttgtttcat caaggccaaa
    12841 aaggttatag ctgattttac catcctttgt tttattatca cctgataatg gaatgtatac
    12901 tttatcagca ctgacccccc aataaccata gggatctttt ttttgattag ctctgggaaa
    12961 aaatattttt ttgttgttat tttgatcttg ttttgaattt aataagtttc taacctcttg
    13021 gcctgcatca agtctttcgg ctgtttggtc tgaaatcata tttagtttgt cttgtttata
    13081 ggtatcgaca ctgtaacctg agcgtttttc aaaaaggtag tttatttctg tatcttttgt
    13141 aacattcccg ccgggatacc agctgagata ggtatgagtt ggttcctgta gtaaattgtt
    13201 ttttatactg atactggtat gccctaaatg ggttatgcct ctatttcccc agcaacgaaa
    13261 cactaaatgt ctttcaagat ttttggctgt accgatgcca tgaaaactct ctgcggtatt
    13321 caacattaat ttgatgtctt tacctgtaaa agaatgttga attgaatttt tgactgcttc
    13381 ataggcggca aacgcacttt ctgcagattt ttcgctagct aatgtcttga ggaaaagcaa
    13441 taaggatttt tgattcgcta gtcgataatt ttttagttgt tcatttaata tctgtgaatt
    13501 tatctttgca ttaatagtgc ttaatgttct gaaaaagaga tcctttaggt tgaatttctg
    13561 tgctaccaat ataggtccat gctctgtatc ttcaacgcgt ataggccctt ttgtattgct
    13621 tgcgtcaatg aaattgctaa ttttgagaat catactttat taactctcca ttacgtggtg
    13681 attgtgggga tgtaaaatgt atttgatata taatatattc aattaataaa tttagaaact
    13741 tgagcctgtt aacataatca aattttcttt tgctgtacat aatatgtacc tcgtgagcat
    13801 atgtagtgct ggtttcatca tgaaatccca caagttaaag tgtctgatat atcaggctcg
    13861 gagtgttata gaaaaagaga gaaccctgtt gaataagtat cttttttgtt gtaaaacgag
    13921 gtacaaaact gcaatcaatg ccccatttat gactgaggat aattaaatgc acataactaa
    13981 tttgggattg catcaggttt catttcaaag cggagattcc tataaaggcg cagaagaaac
    14041 cggaaaccaa aaaggtgtaa gcgtgatttc atatcaaaga gttaaaaatg gagagagaaa
    14101 taagggggtc gaagcgctta atcgattata tttacagaac caaacaagct taacagggaa
    14161 aggccttttg tttgcgcgtg acagagctgc agttttttac gaggcaataa agttggcagg
    14221 tggtgatacg tcaaaaatta aagccatgat ggaacaatta gatacctata aacttggtga
    14281 agttgataaa agaaatatta atgagcttaa taaagtaata agtgaagaga taagagcaca
    14341 gctaggcatt aaaaataaaa aggaattgca atcggaaata aaacaaatat ttactaatta
    14401 tttaaataat aagaattggg aaccgatcaa caaaaatatt aattataatg ggaaaaatta
    14461 tggttttcaa ttaacacccg cctctcatat gaagataggg aataaaaata tatttgtcaa
    14521 agagtataat ggaaaaggaa tttgttgtgc ttctacgaga gagagtaagc atattgcgaa
    14581 tatgtggctt tcaaaagtgg tggatgacga ggggaaggaa attttttcgg gtatcagaca
    14641 tggagttata tcagcctatg gattgaaaaa aaactcatct gaaagagctg ttgcagctcg
    14701 taataaagct gaggagttag caagcgcagc attatatagc agacaagaat tattatcaca
    14761 ggctttgtct ggtaaaacag tagatttaaa gattgtttcg acttctcttc tgacgccgac
    14821 cagtttaact gggggagagg agagtatgtt aaaagatcag gtaaaggcat taaaagagtt
    14881 aaactctaaa aggggggaac caacgaaatt attaattcgg aatagtgatg gtcttctgca
    14941 agaagtaaag gttaatctaa aagtggtgac atttaacttt ggtgtaaatg aattagcgct
    15001 aaaaatgggc ttaggctgga ggaatgttga caagcttaat ggtgaatcaa tatgttcttt
    15061 gttgggggat aatttcctca aaaatggtgt gattggaggt tgggctgcag aagctataga
    15121 aaaaaatcca ccatgtaaga atgatgtgat atatctggct aaccagataa aagagattct
    15181 aaacaagaag ttacaaaaga atgataatgg agagccgtat aaattgtcac aaagaatggc
    15241 tctattggct tatactatag gtgctgtacc ttgctggaat tgtaagagtg ggaaggacag
    15301 aacaggtatg caggatgctg aaattaaaag agaaataata agaaaacatg aaacagggca
    15361 gttttctcaa ttaaatagta aattatcctc agaagagaaa agattatttt ctactattct
    15421 aatgaatagt ggtaatatgg aaatccaaga gatgaatact ggtgtgcctg gaaataaagt
    15481 tatgaaaaaa ttaccgctat cttcgttaga gctgtcttat gctgaaagaa taggggaccc
    15541 caaaatatgg aatatggtga aagggtattc gtcatttgta taagaggaat atatggaaga
    15601 tttagcagat gttatttgcc gagccttggg tatcccttca attgataccg atgatcaagc
    15661 aatcatgctt gatgatgatg tgttaatata tattgaaaaa gaaggagatt caataaattt
    15721 attatgccct ttttgtgcat tgcctgaaaa tataaatgat cttatatatg cgttgagcct
    15781 aaattactca gaaaagatat gtttagctac tgatgatgaa ggtggaagtc taattgcgcg
    15841 attagatctt accgggataa atgaattcga ggatatatat gttaatacag aatattatat
    15901 ttcgcgtgtg cgttggctga aggatgaatt tgcgcgaaga atgaaggggt attaatgtcc
    15961 cgtttcgtat ttatttttct gtgttttatc ccatatttag ggagagctga ttgttgggat
    16021 aaggctggtg aaaggtacaa cattccatcg agtttgttaa aagcgatagc ggaaaaagaa
    16081 tccggattta ataaatttgc agtcaatgtt aataacaatg gaagtaaaga ttatggtata
    16141 atgcaaataa atgactttca ttctaaaaga cttagagaaa tgggatatac tgaggaaatg
    16201 ctaattagtc atccatgtct ttctgtacat tatgcagcaa agttattaaa tgagtttatg
    16261 ctgatgtatg gaagagggtg ggaggctgtt ggtgcctata atgcaggaac atctccgaaa
    16321 aagaaaaaag aaagattaaa gtacgcagag gatatctata aacgctattt gcggatagca
    16381 gcagagagca agcagaataa tcgaaggata taagaggatt tatgtctgag gcaaagaact
    16441 caaatcttgc accattcagg ttattagtga aactcacaaa cggtgtcggg gatgagtttc
    16501 cgttatatta tgggaataac ttaattgttc tcggacggac gatagaaaca cttgagtttg
    16561 gaaacgataa tttcccagaa aatattattc cagtaacaga tagtaaatcg gatggtataa
    16621 tttatttggc cataagtaaa gataatatct gtcaatttag tgatgaaaaa ggtgaacaaa
    16681 ttgatatcaa ttcacaattt aactcatttg aatatgacgg agtttctttt caacttaaaa
    16741 atatgatgga ggataaatcg agagttcata tactaaacgg aatgtataat aaaccctcgg
    16801 tgtttttttt ctttgctgtt attgttgtgt taattataat tttttccctc tctttaaaga
    16861 aagatgaagt aaaagaaatt gctgaaatta tagatgataa gagatatgga atagtcaaca
    16921 caggacagtg caattatatt cttgccgaaa cacaaaatga tgcggtatgg gcatctgtag
    16981 ccttaaataa aagcggattt accaaatgca gatatattct ggtgtccaac aaagaaataa
    17041 atagaattca acaatatatc aatcaacgat tcccatatat caatttgtat gtgttgaatt
    17101 tagtgagtga taaagcggaa ttactggtat ttttaagtaa agaaagaaat agttcaaaag
    17161 ataccgaact tgagaaattg aaaaatgctc tgatagttga atttccgtat atcaaaaata
    17221 ttaaattcaa ctatcttagc gatcataatg caagggagga tgctaaaggt attttcacaa
    17281 aggtaaatgt ccagtataaa gagatttgtg aaactaataa agttacatat tctgtcagag
    17341 aagaactgac agatgaaaag cttgagctca ttaatagact gatttctgat cataaaaatg
    17401 tatatggaga tcagtatatt gagttttctg tacttttgat agatgatgat tttaaaggta
    17461 aatcatatct taacagcaaa gacagttatg taatgttgaa tgataaacac tggttttttt
    17521 tagataaaaa taagtgagga taaaatgagt gttacagtac cggataaaga ttggactctg
    17581 agttcattat ctgaaacttt tgatgatgga actcaaacat tacaagggca actaacatcg
    17641 gcactaaatg cattagctga aaatccttcg aatccacagt tgctggctga ataccaaagt
    17701 aaattatctg aatatacatt atataggaac gcgcaatcta atactgtgaa agtgattaag
    17761 gatgttgatg ctgcaattat tcaaaacttc agataatagg gagcattcat gaattacatt
    17821 tatccagtca atcaggttga tattatcaaa gccagtgatt ttcaatctca agagatatca
    17881 agcctggaag acgtcgtgtc ggctaaatat agtgatatta agatggatac agatattcaa
    17941 gtatcacaaa taatggagat ggtaagccat ccagaatcat taaacccaga atctttggcc
    18001 aagttacaga cgacgctctc aaattattca ataggagtat cattagctgg cacgttagca
    18061 agaaaaacag tttcggctgt tgaaacttta ttaaagtctt aatttatatg attaggtata
    18121 aatgttttat tttattcttg ttgctgatgt tgattggatg tgagcaacgt gaagagttaa
    18181 tttctaattt atctcaaaga caggcaaatg aaataatatc tgtgctagaa cgccataata
    18241 ttactgctag aaaagttgat ggaggtaaac aggggatctc ggtacaagtc gaaaagggga
    18301 catttgcatc ggcagttgat ttgatgcgca tgtacgattt gccaaatccg gagagagttg
    18361 atatctcaca aatgtttcct acagattcat tagtgtcttc tccaagagct gaaaaggccc
    18421 gtttatatag tgctattgag caacggctgg aacagtcttt agtttctatt ggtggtgtta
    18481 tttcggcaaa aatacatgtt agctatgatc ttgaagaaaa aaatatatct tcaaaaccga
    18541 tgcatatatc agtaatcgct atatatgact caccgaaaga gtctgaacta ttagttagta
    18601 atattaagcg atttttgaaa aacacctttt ctgatgttaa gtatgaaaat atatctgtca
    18661 tattaactcc gaaagaagaa tatgtttata caaatgtaca acctgttaag gaaattaaat
    18721 cggaattttt aacaaatgaa gtaatatatg tatttctcgg gatggctgta ctagttgtca
    18781 ttcttttggt atgggcattc aaaacagggg ggttcaagag aaacaaaata tgataagaat
    18841 ggatggaatt tataaaaaat atctttcaat aatttttgat ccagcgttct atataaatag
    18901 aaatcggttg aatttgcctt ctgaactgtt agaaaatggc gtaatcagaa gtgagattaa
    18961 taatctcata attaacaaat atgatctaaa ttgcgatatt gaacctttaa gcggggtaac
    19021 cgctatgttt gttgccaact ggaatttact tccagctgtt gcttatttta tcggtagtca
    19081 agagtctcgg ttaattaatc atagtgagat ggttattagt tattatgggg gcaaaatcag
    19141 taagcaaggg gaggccgcta ttcgaagtgg tttttggcat cttattgagt ggaaggaaaa
    19201 tatatctgtt ggtatatatg aaagaataaa cttattattt aatccaattg ctttggaggg
    19261 aaattatacg cctgtagaaa gaaatttgtc ccgattaaat gaaggtatgc aatatgcaaa
    19321 aaggcacttt accggtatcc agacatcatg cctatgatgg tgttgttata aagagaattg
    19381 aaaaggaatt atgtaaaacc ataaaagata gagacactga gagtaagaag aaagcaattt
    19441 gtgttataaa ggatgctacg aaaaaagctg aatcactgcg tatagatgct gtatgcgatg
    19501 gttatcaaat tggtatacag actgcttttg agcatattat tgactatatt tgtgaatgga
    19561 agttaaagca aaatgaaaac agaagaaata ttgaggacta tataactagt ttgctttcag
    19621 aaaatttaca tgatgagagg atcattagca ctttattgga gcagtggctt agttcgttgc
    19681 gtaatacagt aacagaatta aaagtggttt tgcccaagtg taatttagca ctgaggaaaa
    19741 agttagaact tgatctccat aaatatcgct cagatgtcaa gattatttta aaatattctg
    19801 aagggaataa ttatatattc tgtagtggta atcaagtagt ggagtttagt cctcaggatg
    19861 taatatcagg tgttaaaata gaacttgcgg aaaaattaac aaaaaatgat aaaaaatatt
    19921 tcaaagagtt agcacataag aaactgagac aaattgcaga agacttgtta aaagagaatc
    19981 ctgttaatga ttaatcaaat aaatgcaagc aatgctctgc agcaaagatt aaatagtgaa
    20041 gaatttgtta atttaaatga gagattatca tcttcgcaat catttgatga gaatataatc
    20101 tatgaaataa tgcaatattt ctcacagagt gaattaaatt ccatagataa tgatgaactg
    20161 cacaataaga ttgaacaatt attcaattca cgttttcctt atcttacagc tgcacaaaaa
    20221 agttcacttc ttaataaact catagatgca aatcaatatg tcgatttgca tgagggattt
    20281 tatgcatcat tgtccatata taacaatatt gatttctata ttaagactac aaccttcgat
    20341 tcattaatta gtgtctttga agcagggaga gaggcagatg attcgacatg gtagtaataa
    20401 gttgaaaata tttattttaa gtatattgct attaacactg agtgggtgtg ctttaaagtc
    20461 atcatctaat tctgaaaaag aatggcatat tgttcctgta agtaaggatt atttttctat
    20521 tccaaatgat ttattatggt cgtttaatac aaccaataaa agtataaatg tttactctaa
    20581 atgtattagt ggtaaggcgg tttatagttt taatgcaggt aaattcatgg gcaactttaa
    20641 tgttaaggaa gtagatgggt gcttcatgga tgcacaaaag atagctatag ataaactatt
    20701 ttctatgctg aaagacgggg ttgttttaaa aggtaataag ataaatgata ccatccttat
    20761 agagaaggat ggggaagtta aattaaaatt aattcgaggg atataattgt attgtgagta
    20821 aatataaagg tctaaataca agtaatatgt tttacattta ctctagtgga cataaaccag
    20881 ttaacgttga gcttgtaaaa gataaagaac gtaacataat tgagctggct ccagcatgga
    20941 agggtttttt tttgtccgaa atcaaaatat aaaattcagt gataatgtga actatcatta
    21001 tcgctttaac attaatagtt gtgcaaaatt tcttgctttt tgggactatt tcagtggtgc
    21061 tctagtagag catagccatg ctgaaaaatg tattcatttc tatcatgaga atgatcttcg
    21121 ggatagttgt aataccgaat cgatgcttga taagttaatg ttaagattca tcttttcatc
    21181 tgaccagaat gtaagtaatg cactggctat gatacgaatg actgagtcat atcatttggt
    21241 tctttatctt ctgagaacta ttgagaaaga aaaggaggta agaataaaga gtctgacaga
    21301 gcattatggt gtgtcggagg cttattttag atctttatgt agaaaagctt taggggcaaa
    21361 agtcaaagaa caactgaaca cttggcggtt agtaaatggt ttattagatg tatttttgca
    21421 caatcaaaca atcacttctg ctgccatgaa taatgggtat gcatcaacat cccatttttc
    21481 aaatgagata aaaacacgac taggcttttc tgcaagagaa ttatcgaata ttactttttt
    21541 agtgaaaaaa ataaatgaaa aaatttaata ttaaatcttt gactctcttg attgtattgt
    21601 taccattgat agttaatgct aataatattg actctcattt attagaacaa aatgacatag
    21661 cgaaatatgt tgctcaaagt gatacggtag gatctttttt tgaacgtttt tctgcgttat
    21721 taaattatcc tattgttgtt agcaaacaag ctgcaaaaaa gagaatatct ggggagtttg
    21781 atttatctaa tccagaagaa atgcttgaaa aaatgacatt acttgtggga ctcatatggt
    21841 ataaagatgg aaatgcatta tacatatatg actctggaga attaataagc aaagttattt
    21901 tgctggagaa tatatcactt aattatctta ttcagtatct gaaggatgct aacctttatg
    21961 atcacagata tcctataaga ggaaacatta gtgataaaac attctatata tctggtcctc
    22021 cggcattggt tgaactggta gctaatacag caactttgct tgataagcag gtttcttcta
    22081 ttgggacaga taaagttaat tttggggtta taaaattaaa aaatacattt gttagtgaca
    22141 gaacttataa tatgagaggg gaggatattg tcatcccagg ggtggctact gttgtagaac
    22201 gtttattaaa taatggtaag gctctgagta acagacaagc caaaaatgat ccgatgccac
    22261 cctttaatat tacacagaaa gtatctgagg atagtaatga cttttcattt agttcagtta
    22321 ccaatagttc tatattagag gatgtatcac ttattgcata tccagaaacc aattctatat
    22381 tagtcaaagg taatgatcag caaatacaga ttattcgcga tattatcact cagttagatg
    22441 tagccaaacg acatatagaa ttatcattat ggataatcga tattgataaa tctgaattaa
    22501 ataaccttgg cgttaattgg caaggaacgg cttcatttgg tgattcgttt ggtgcatcat
    22561 ttaatatgag ctcatcagca tcgataagca ctttggacgg caataagttt attgcttcag
    22621 ttatggcatt aaatcagaaa aagaaagcaa atgtagtttc aaggccggtg attttgacac
    22681 aagagaatat tccggctata tttgataata acaggacttt ttatgtgtca ttagttggtg
    22741 agcgcaattc ttcgttggag catgttacgt atggaacatt gataaatgtt atacctcgct
    22801 tttcatctca gggacaaatt gagatgtctt taacgataga ggatggaaca ggaaatagtc
    22861 agtcaaatta caattataat aatgaaaata catctgtcct tccagaagtt ggaaggacca
    22921 aaatcagtac aatagcgaga gttccacaag gcaaaagttt gttgataggt ggttatacgc
    22981 atgaaacgaa ctctaacgaa atagtatcta ttccttttct ctcctctatt cctgtaatcg
    23041 gtaatgtgtt caagtataaa acaaggaata tcagtaatat tgtaagggtt tttctgattc
    23101 aaccccgtga gataaaggag tcaagttatt ataatactgc agaatataag agcctcatat
    23161 ctgaaagaga aatacaaaag actacacaaa taatcccatc tgaaactact ttgctggaag
    23221 acgaaaaatc attggtttca tacttaaatt actaactata aagtaggtga tgtatgcttg
    23281 atgttaaaaa tacaggagtt tttagctctg cattcattga taagttgaat gcaatgacaa
    23341 attcagatga tggagatgag actgctgatg cagagcttga ttctggcttg gctaatagca
    23401 agtatattga ctcatctgat gagatggctt ccgctctttc gtcatttata aacagaagag
    23461 accttgagaa actgaaagga acaaatagtg atagtcagga acgtatttta gatggggaag
    23521 aagatgaaat taatcacaag atttttgatt taaagagaac tttaaaagat aaccttcctc
    23581 tagatcagga ttttatagac agactaaaga gatattttaa agatccaagt gatcaagtct
    23641 tagcattaag ggaacttttg aatgaaaaag atcttactgc tgaacaagtc gaatcattaa
    23701 ctaaaattat taatgagata atatcaggta gtgaaaaaag tgttaatgct ggaataaatt
    23761 cagctataca ggctaaatta tttggcaaca aaatgaaact tgaaccgcag cttttgcgtg
    23821 catgttatcg tggttttatc atggggaaca tatcaacaac agatcagtat atagaatggc
    23881 ttggtaattt tggttttaat cacagacata caattgtgaa ttttgtagag cagtcactga
    23941 ttgtagacat ggattctgag aaaccgagct gtaatgctta tgagtttggt tttgtgttat
    24001 ctaaattaat tgcaattaag atgattagaa cttcagatgt aatttttatg aagaaactgg
    24061 aatcctcaag cttgctaaaa gatggcagtt taagtgcaga gcagctattg ctaactttat
    24121 tatatatttt tcaatatcca agtgaaagtg agcaaattct tacttctgtt atagacgtat
    24181 cacgagccag tcatgaggat tctgtagtgt atcaaacata tctatcttct gttaatgaaa
    24241 gtcctcatga tatatttaaa agtgaaagtg aaagagaaat tgcgatcaat attctacgag
    24301 agcttgtcac aagtgcatac aagaaagagc tttctagata acaggagatg aaagtgatcc
    24361 agtcttttct taagcaagta agtactaagc ctgaattaat tatattagtg cttatggtta
    24421 tgatcattgc tatgttgatc ataccattac ctacatacct tgttgatttt ttaatagggc
    24481 ttaatattgt acttgctatt ctcgttttta tgggatcatt ttatattgaa agaatattga
    24541 gtttttctac attcccttcg gttttgttga taacgacatt atttcgtctc gctttatcaa
    24601 taagtactag tcgactaatt ttagttgatg cggatgcggg taaaattatt actacatttg
    24661 gccagtttgt cattggtgat agtctggctg taggttttgt tattttttca atagtaactg
    24721 ttgtgcagtt cattgttata acaaaaggat ctgaaagggt tgcggaggta gctgctcgct
    24781 tttctcttga cggtatgccg gggaaacaaa tgagcattga tgcggattta aaggctggaa
    24841 ttattgatgc cgcaggagct aaagaaagac gcagtattct ggaacgtgag agtcaattgt
    24901 atggttcatt tgatggagca atgaagttca ttaaaggtga tgcaattgct ggcatcatta
    24961 ttatttttgt caatttaata ggaggtattt ctgttggtat gagtcaacat ggaatgtctc
    25021 tatctggtgc attatctact tataccatac taactattgg tgacggattg gtctctcaga
    25081 ttcctgctct gttaatttct ataagtgcag gatttatcgt tacaagagtt aatggtgata
    25141 gcgataatat gggacgtaac ataatgtccc agatatttgg gaatcctttt gttctcattg
    25201 ttacatcagc tcttgccttg gcgataggga tgttgccagg ttttccgttt tttgtttttt
    25261 tcctgatagc agttactttg acggctttat tctattataa aaaggtcgta gaaaaagaaa
    25321 aaagtctgtc tgagtctgat tctagtggct atactggtac atttgatatt gataatagcc
    25381 atgactcatc tttggcaatg atagaaaatc tggatgctat tagttcagaa accgttcctt
    25441 taattttatt atttgccgaa aataagataa gtgcaaatga tatggaaggt cttattgaaa
    25501 ggataagaag tcagttcttt attgattatg gtgtcaggct tccaactatt ttatatagaa
    25561 caagcaatga gcttaaggtc gatgatattg ttttgttaat aaatgaggtg cgtgccgata
    25621 gttttaatat atactttgac aaagtgtgta ttacagatga aaatggagat atagatgctc
    25681 taggtattcc tgttgtttca acttcatata atgaacgtgt tatttcttgg gtagacgttt
    25741 catatacaga aaatctaact aatattgatg ctaaaattaa aagtgctcaa gatgaatttt
    25801 atcaccagtt gtcacaagct ttattaaaca acataaatga gatttttggt atacaagaaa
    25861 caaaaaatat gttagatcag tttgaaaatc ggtatcctga tctattaaag gaagtcttcc
    25921 gacatgtgac tatacagaga atttctgagg tattacaaag attgcttgga gaaaatattt
    25981 ctgttcgcaa tttaaaactt attatggagt ctttggcgct ttgggctccc agagaaaaag
    26041 atgtcataac attagttgaa catgtccgtg catcactttc taggtatatt tgtagtaaaa
    26101 tagctgtttc tggtgagatt aaagttgtga tgctttccgg atatattgag gatgcaataa
    26161 gaaaagggat aaggcaaacc tctggtggct ctttcttgaa tatggatata gaggtttcgg
    26221 atgaggtaat ggaaacttta gcacatgctt tgagagaatt gagaaatgca aaaaaaaatt
    26281 tcgttctttt ggtatcagta gatatacgta ggtttgttaa aagacttata gataacagat
    26341 ttaagagtat actcgttata tcgtatgctg agattgatga agcatatacc attaatgtat
    26401 taaagactat ttagtgaggt ttaaatatga gtaacattaa tttagttcaa ttagttagag
    26461 atagtctttt cacgattggt tgtcctccat caattattac agatttagac agccattcag
    26521 cgattaccat ttctttggat agtatgcctg ctattaatat agctcttgtc aatgagcagg
    26581 ttatgttatg ggctaatttc gatgcaccaa gtgatgtaaa acttcagtca tctgcttata
    26641 acatattaaa tttaatgtta atgaacttta gttatagcat taatgagctg gtagagcttc
    26701 atcgttctga tgagtactta cagttaagag ttgttattaa agatgactac gttcacgatg
    26761 ggattgtttt tgcagagatt ctgcatgagt tttatcagag gatggaaatc ttaaatgagg
    26821 tcttataatc aatgagctat ataaaattgc tcactcaatt atcttttcct aatagaatct
    26881 cggggccaat cttggaaaca agtcttagcg atgtttcgat tggtgagatt tgtaacattc
    26941 aggctggaat tgaaagtaat gaaattattg caagagctca ggttgtagga tttcatgatg
    27001 aaaaaacaat attaagcttg attggaaatt ctcgtggact ttcacggcaa acgttgatta
    27061 agcccactgc ccagtttctt catacgcaag tcggccgtgg attattggga gcagtagtca
    27121 atcctttagg ggaggttact gataagtttg ctgttacgga taacagtgaa attctttatc
    27181 gacctgtaga taatgctcct ccgctatata gtgaaagggc tgcaattgag aagccttttt
    27241 taacaggtgt taaggttatt gattctttac tcacgtgtgg tgaaggacag cgaatgggga
    27301 tttttgcgtc agctggttgt ggcaaaactt ttctcatgaa tatgctcatt gaacatagtg
    27361 gtgctgatat atatgttatt gggttaattg gtgagcgagg tcgagaggtt acagaaacgg
    27421 ttgattattt gaaaaactct gagaaaaaaa gcaggtgtgt tttagtatat gcaacttcgg
    27481 attactcttc ggttgatcgt tgtaatgctg catatatagc cactgctata gccgaatttt
    27541 ttaggactga aggacataaa gtagcgcttt ttattgattc attaacaagg tatgccagag
    27601 cattacgtga tgtggcctta gccgctggag aatcacctgc cagaagaggc tatccggttt
    27661 cggtttttga tagcttaccc agacttcttg aaaggccagg aaagttaaag gcaggtggct
    27721 ctattactgc attttacact gttcttttgg aggatgatga ttttgctgat ccattagctg
    27781 aagaggtaag atccatttta gatggacata tatatttgag cagaaatcta gcccaaaaag
    27841 gacaatttcc tgcaattgat tccttaaaaa gtataagcag ggtatttaca caggttgttg
    27901 atgaaaaaca tcgtattatg gccactgcat ttcgggagct actttctgaa atagaagagc
    27961 taaggacaat tattgacttt ggtgaataca aaccggggga gaatgcctct caggataaaa
    28021 tctacaacaa aatatctgtt gttgagagtt ttctgaagca agattatcga ctcggtttta
    28081 cttatgagca gacaatggag cttcttggtg aaacaattag ataaggtttt aaaaatcaaa
    28141 gataaatatc aacggtctgt taaattaatt gaagcacaca tattaacact aaaaaaaaaa
    28201 ctctttgtat cgagatgtgg aggcattaga taaaaggatt atatacttcc tgcaactgga
    28261 gaatgacttg gaacctgtag gcgctcagag tgttagtcag cttttcaata caagacgtaa
    28321 gatcgctata gtaaaaaagc atattatcca atatcagtct gaacgaattc ttctaaaggg
    28381 aagaattgaa gaaattcaaa aagatattga tgaggcaaat gcttctaaaa gaaaactgct
    28441 tcataaagag agtaaaatat gtaaacggat tggcttgatt aaaagaaata actttgccaa
    28501 acagttgata cttgatgagt tatcacaaga ggatatgaag tatggcatta gataatataa
    28561 acctaaattt tagtagtgac aaacagatag aaaaatgtga gaaactatct tcaatagata
    28621 atattgatag tctcgttttg aagaagaaga ggaaggttga aattcctgag tactctttaa
    28681 ttgcatctaa ctattttact attgataagc actttgaaca taagcatgat aaaggagaaa
    28741 tttatagtgg cattaaaaat gcgttcgaac ttagaaacga acgagcgaca tattctgata
    28801 ttccagaatc aatggccatt aaagaaaata ttttgatacc agatcaagat atcaaagcaa
    28861 gggaaaaaat aaatatcggc gatatgaggg ggatcttttc atataataag agtggaaatg
    28921 cagacaagaa cttcgaaaga agtcatactt cttctgtaaa ccctgataat ctgctagaat
    28981 ctgataatag aaatggtcaa attggtttaa aaaatcatag cttgtctatt gataagaata
    29041 ttgctgacat catttcttta ctaaatggaa gtgttgctaa atcatttgag ctgcctgtaa
    29101 tgaataaaaa tactgcagac ataaccccat ccatgtcatt gcaagaaaaa tcaatagttg
    29161 aaaatgataa aaatgttttt caaaaaaata gtgaaatgac ttaccacttt aaacagtggg
    29221 gggctggaca ttctgttagt atttcagtgg agtctggttc ttttgttcta aaaccgtcag
    29281 atcaatttgt aggaaataaa cttgacttaa ttttgaaaca agatgctgag ggtaattaca
    29341 gatttgatag cagtcaacat aataagggga ataaaaataa tagtacagga tataatgaac
    29401 agagtgaaga agaatgctaa gaattaaaca ttttgacgct aacgaaaaac tacagatttt
    29461 atatgcaaag caactctgcg agcgtttttc gattcagaca ttcaaaaata aatttacagg
    29521 cagtgaaagt ttagtcactc ttacttccgt gtgtggggat tgggtaattc gtattgatac
    29581 attatctttt ttgaaaaaaa aatacgaggt attttcagga ttttctacac aagaatcttt
    29641 actgcattta tcaaaatgtg tctttataga gtcgtcatct gtattttcga ttccagaact
    29701 gtctgataag attactttcc ggatcacgaa tgaaatccag tatgcaacta ctgggagtca
    29761 tttatgctgt ttttcctctt ctttaggtat tatttatttt gacaagatgc cggtattacg
    29821 taatcaagtt tctcttgact tattgcatca tcttttagag ttttgcttag gttcatctaa
    29881 tgtaaggctg gctactttaa aaagaattcg tactggtgat ataatcatag ttcagaaact
    29941 ttataattta ttattgtgta atcaagttat tattggggat tatattgtga atgataataa
    30001 tgaggcaaaa attaatctgt cagaaagtaa tggtgagtca gatcacacag aagtttcttt
    30061 ggcattattc aattatgatg atatcaatgt aaaagtggac tttatccttt tagaaaaaaa
    30121 gatgacaatc aatgaactaa aaatgtatgt agaaaacgaa ttattcaagt ttcccgatga
    30181 catagttaaa catgtaaata ttaaagtaaa tggttctttg gttgggcatg gggaacttgt
    30241 ttctattgag gatggttatg gtatcgagat tagttcttgg atggtaaagg agtaatctca
    30301 tgctgagtga tatgtccctc atcgctacac tttctttttt tacgctatta ccatttttag
    30361 ttgctgctgg tacttgttat ataaagtttt ctattgtttt tgtaatggta cgaaatgcac
    30421 ttggtttgca gcaggttcca tcaaatatga ctctcaatgg tattgcattg attatggctt
    30481 tgttcgtgat gaaacccatt attgaagctg gttatgaaaa ttatctaaat ggcccgcaaa
    30541 aatttgatac aatatcagat attgtccgtt tttctgattc tggcttgatt gaatacaagc
    30601 aatacttgaa aaagcataca gatcttgagt tggcacgttt tttccaaagg tctgaagaag
    30661 aaaatgctga tttaaagagt gcagagaata atgattattc attgttttcc ctcttgcctg
    30721 catatgcgct aagtgaaatt aaagatgcgt tcaaaatagg tttttattta tatttgccat
    30781 ttattgttgt tgatcttgtt atttcaagca ttttacttgc acttggtatg atgatgatga
    30841 gtccaatcac aatatcagta ccaataaaac ttgtattatt tgttgcgctt gatgggtggg
    30901 ggatcctgtc taaggccttg attgaacaat atatcaatgt tcctgcttag atgatggagg
    30961 taatgtgtct gatatagttt atatgggtaa taaggctctt tatttaatcc ttatcttttc
    31021 cttatggcca gtaggtatag ctacggttat tggattaact attggtttat tacagacagt
    31081 gactcaactt caagagcaga cacttccttt tggtataaag cttataggtg tctcaatatc
    31141 tttgctactt ctttctggat ggtatggtga ggttttattg tctttttgtc atgaaataat
    31201 gtttttaatt aagagtgggg tttgatggac atttcaagct ggttcgaaag tattcatgtg
    31261 tttttaatac tcctgaacgg cgtttttttt agattggctc cattattttt ctttcttcca
    31321 tttttaaata acggtataat ttctccatct attagaatac ctgtgatttt tcttgttgca
    31381 tcaggattaa ttacttctgg taaggtagac ataggttctt ctgtttttga acatgtttat
    31441 ttccttatgt tcaaggaaat aattgttggc ctccttctct ctttttgctt gtctcttccc
    31501 ttttggatat ttcatgctgt tggtagtatt attgacaacc agcgtggggc aacgcttagt
    31561 agttcaattg atcctgccaa tggtgttgat acgtctgaat tggcaaaatt tttcaatctt
    31621 ttttctgcag ttgtatttct atacagtggt ggaatggtct ttattttaga atccatacaa
    31681 ttgtcttata atatatgccc gttattttct caatgttctt tccgtgtctc aaatatctta
    31741 acatttctga ctttattggc aagtcaggct gttattttag ccagtcctgt tatgatagta
    31801 ttgttactat cagaagtatt acttggtgta ttatcgagat ttgctccgca gatgaatgct
    31861 ttttccgtat cattaactat taaaagttta cttgcaatat ttattatttt catctgttct
    31921 tctactattt acttttctaa agttcaattt ttcctcggtg aacataaatt tttcacaaat
    31981 ctatttgtta gataaaatat tatggcaaat aaaacagaaa agccgacacc taaaaaacta
    32041 aaggatgccg caaaaaaagg acagtcattt aaatttaagg atttaacgac agttgttatt
    32101 attctggtag ggacatttac tataatatca ttcttttcct taagtgatgt aatgctctta
    32161 tacagatatg taataattaa tgacttcgaa attaatgagg gtaaatactt ttttgcagtg
    32221 gttattgtct tttttaagat aattggcttc ccactttttt tctgtgttct ttcggctgtg
    32281 ttgccaacat tggttcaaac aaagtttgtt cttgcgacta aagctatcaa gattgatttt
    32341 tcagtattaa accctgttaa agggttaaaa aaaatattta gtataaagac aataaaagaa
    32401 tttttcaaaa gcattctgct tcttattatt ctagcattaa caacctattt cttttggatt
    32461 aatgaccgaa aaataatttt ttctcaggtg ttttctagtg ttgatggctt atatcttatt
    32521 tggggggggc tatttaagga tataatatta tttttcttag cattttctat ttttgttatt
    32581 attcttgact ttgtgattga gttcatttta tacatgaaag atatgatgat ggataaacag
    32641 gagataaaaa gagaatatat agagcaagag ggacactttg agacaaagtc gagaaggcgt
    32701 gagttgcata tcgagattct ttcagagcag actaaatctg atatacgtaa ttcaaaatta
    32761 gtggtaatga acccgactca tattgcaatt ggtatttatt ttaatccaga aatagcgcct
    32821 gcacctttta tttctctcat tgaaactaac cagtgtgcct tggctgtcag aaaatatgca
    32881 aatgaagttg gtataccaac tgtgcgtgat gtgaaattag ctagaaaact atataaaaca
    32941 catacaaaat atagttttgt tgattttgaa cacttggatg aagtcctacg tcttattgtt
    33001 tggcttgagc aggttgaaaa cactcattaa taagtaaagg agatgtgtta tatgggagtt
    33061 aatttctgta ataaaatagg tattgatcag tctgaatttg aaatagaatc ttccatcatt
    33121 aactccattg ctaatgaggt attgaaccca atatctttcc ttagcaacaa ggatataata
    33181 aatgttttac tcagaaagat ttcatctgaa tgtgacctgg ttagaaaaga catttatcgt
    33241 tgtgctcttg agttggtcgt tgaaaaaaca cctgatgatt tataatcatt cataataact
    33301 cttttgttga tataaatatt tttgttgaag atatttatgt agtcacagga tcaattgtag
    33361 aaaattgtta tgtttttgtt aggaactgaa ctaaataata tatgataaga cagcaaaaac
    33421 gactgaccat aatccttctt ttattaggag ttgataagag agactattca tcctgtaatg
    33481 ttaaaactct gttatatagt attcgcgatt atgcaaagtc tgtaaatgat catgagatac
    33541 taaccgagag taatcgttta ttatctcatt gcatttcaga tagtaacgga gctttcttta
    33601 aaagtagtaa atacgttccc cttaagtact tacgtaaaag gagaatagca cgaaagatac
    33661 caaatgatta gcactttgct agtaagcttg ctggtaaact atattgaccg ttgggtttgt
    33721 tttgagactg taatttaaat tgtgtaattg cctgtttttg atatgtttac tccaacaacg
    33781 gagacaggta aattatggac gaaaagaagc ttaaagccct tgcggctgaa ctggctaaag
    33841 gcctcaaaac tgaggccgac ctcaatcagt tttcccgtat gctgacgaaa tatcccggct
    33901 cttaaaacga tagtggctca ggctttcatt tttcagcgtt ccccagaag
//
LOCUS       DAJVUJ010000025        33946 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.025, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000025 DAJVUJ010000000
VERSION     DAJVUJ010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33946
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.025"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(270..482)
                     /gene="rpmE"
                     /locus_tag="OQQ48_001125"
     CDS             complement(270..482)
                     /gene="rpmE"
                     /locus_tag="OQQ48_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HCS1951971.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            685..2883
                     /gene="priA"
                     /locus_tag="OQQ48_001126"
     CDS             685..2883
                     /gene="priA"
                     /locus_tag="OQQ48_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCS1951972.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNACPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPMFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWILGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            3039..4064
                     /gene="cytR"
                     /locus_tag="OQQ48_001127"
     CDS             3039..4064
                     /gene="cytR"
                     /locus_tag="OQQ48_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HCS1951973.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4156..5115
                     /gene="ftsN"
                     /locus_tag="OQQ48_001128"
     CDS             4156..5115
                     /gene="ftsN"
                     /locus_tag="OQQ48_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HCS1951974.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5208..5738
                     /gene="hslV"
                     /locus_tag="OQQ48_001129"
     CDS             5208..5738
                     /gene="hslV"
                     /locus_tag="OQQ48_001129"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HCS1951975.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            5748..7079
                     /gene="hslU"
                     /locus_tag="OQQ48_001130"
     CDS             5748..7079
                     /gene="hslU"
                     /locus_tag="OQQ48_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HCS1951976.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            7146..8072
                     /gene="menA"
                     /locus_tag="OQQ48_001131"
     CDS             7146..8072
                     /gene="menA"
                     /locus_tag="OQQ48_001131"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCS1951977.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDRPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFILGIFLSQWAA"
     gene            8165..8650
                     /gene="rraA"
                     /locus_tag="OQQ48_001132"
     CDS             8165..8650
                     /gene="rraA"
                     /locus_tag="OQQ48_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HCS1951978.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(8735..8980)
                     /gene="zapB"
                     /locus_tag="OQQ48_001133"
     CDS             complement(8735..8980)
                     /gene="zapB"
                     /locus_tag="OQQ48_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HCS1951979.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            9406..10251
                     /gene="glpF"
                     /locus_tag="OQQ48_001134"
     CDS             9406..10251
                     /gene="glpF"
                     /locus_tag="OQQ48_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HCS1951980.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVKEKETTTPSE
                     QKASL"
     gene            10274..11782
                     /gene="glpK"
                     /locus_tag="OQQ48_001135"
     CDS             10274..11782
                     /gene="glpK"
                     /locus_tag="OQQ48_001135"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCS1951981.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            11917..12927
                     /gene="glpX"
                     /locus_tag="OQQ48_001136"
     CDS             11917..12927
                     /gene="glpX"
                     /locus_tag="OQQ48_001136"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCS1951982.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAVLGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            13024..13770
                     /gene="fpr"
                     /locus_tag="OQQ48_001137"
     CDS             13024..13770
                     /gene="fpr"
                     /locus_tag="OQQ48_001137"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HCS1951983.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(13775..14203)
                     /gene="uspD"
                     /locus_tag="OQQ48_001138"
     CDS             complement(13775..14203)
                     /gene="uspD"
                     /locus_tag="OQQ48_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HCS1951984.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(14230..14529)
                     /locus_tag="OQQ48_001139"
     CDS             complement(14230..14529)
                     /locus_tag="OQQ48_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HCS1951985.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(14741..15181)
                     /locus_tag="OQQ48_001140"
     CDS             complement(14741..15181)
                     /locus_tag="OQQ48_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCS1951986.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     CFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            15282..15881
                     /locus_tag="OQQ48_001141"
     CDS             15282..15881
                     /locus_tag="OQQ48_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HCS1951987.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            15989..16756
                     /gene="tpiA"
                     /locus_tag="OQQ48_001142"
     CDS             15989..16756
                     /gene="tpiA"
                     /locus_tag="OQQ48_001142"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCS1951988.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(16811..17566)
                     /gene="cdh"
                     /locus_tag="OQQ48_001143"
     CDS             complement(16811..17566)
                     /gene="cdh"
                     /locus_tag="OQQ48_001143"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HCS1951989.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(17673..18662)
                     /gene="sbp"
                     /locus_tag="OQQ48_001144"
     CDS             complement(17673..18662)
                     /gene="sbp"
                     /locus_tag="OQQ48_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HCS1951990.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVMRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(18982..19944)
                     /gene="pfkA"
                     /locus_tag="OQQ48_001145"
     CDS             complement(18982..19944)
                     /gene="pfkA"
                     /locus_tag="OQQ48_001145"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCS1951991.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGSMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(20125..21027)
                     /gene="fieF"
                     /locus_tag="OQQ48_001146"
     CDS             complement(20125..21027)
                     /gene="fieF"
                     /locus_tag="OQQ48_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HCS1951992.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTPMIDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(21175..21699)
                     /gene="cpxP"
                     /locus_tag="OQQ48_001147"
     CDS             complement(21175..21699)
                     /gene="cpxP"
                     /locus_tag="OQQ48_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HCS1951993.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQRS
                     TQRSTQSHMFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENA
                     VRAQAEKMANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKS
                     SSLKLLSSSNSRSQ"
     gene            21849..22547
                     /gene="cpxR"
                     /locus_tag="OQQ48_001148"
     CDS             21849..22547
                     /gene="cpxR"
                     /locus_tag="OQQ48_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HCS1951994.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            22544..23917
                     /gene="cpxA"
                     /locus_tag="OQQ48_001149"
     CDS             22544..23917
                     /gene="cpxA"
                     /locus_tag="OQQ48_001149"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HCS1951995.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(24023..24697)
                     /gene="yiiM"
                     /locus_tag="OQQ48_001150"
     CDS             complement(24023..24697)
                     /gene="yiiM"
                     /locus_tag="OQQ48_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HCS1951996.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNVYMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(24846..25811)
                     /gene="kdgT"
                     /locus_tag="OQQ48_001151"
     CDS             complement(24846..25811)
                     /gene="kdgT"
                     /locus_tag="OQQ48_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HCS1951997.1"
                     /translation="MRKSRGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMITGTVSI
                     LAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVEVGFFAG
                     LSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGTAGIASF
                     EPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTVIAQTGL
                     LGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPMLIAEMVPAFKP
                     MAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(26090..26710)
                     /gene="sodA"
                     /locus_tag="OQQ48_001152"
     CDS             complement(26090..26710)
                     /gene="sodA"
                     /locus_tag="OQQ48_001152"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HCS1951998.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            26994..28028
                     /gene="rhaT"
                     /locus_tag="OQQ48_001153"
     CDS             26994..28028
                     /gene="rhaT"
                     /locus_tag="OQQ48_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HCS1951999.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPNFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLLNTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     IIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNAGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(28025..28891)
                     /gene="rhaR"
                     /locus_tag="OQQ48_001154"
     CDS             complement(28025..28891)
                     /gene="rhaR"
                     /locus_tag="OQQ48_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HCS1952000.1"
                     /translation="MAEVATVAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDF
                     CELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKL
                     NLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVSFANEMAELLFGQ
                     LVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQ
                     FRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMT
                     PSQWRHLNSQKD"
     gene            complement(28947..29783)
                     /gene="rhaS"
                     /locus_tag="OQQ48_001155"
     CDS             complement(28947..29783)
                     /gene="rhaS"
                     /locus_tag="OQQ48_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HCS1952001.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            30071..31483
                     /gene="rhaB"
                     /locus_tag="OQQ48_001156"
     CDS             30071..31483
                     /gene="rhaB"
                     /locus_tag="OQQ48_001156"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HCS1952002.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWNVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRSNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPEAMCSEIQAACRETAQPIPESDAELAQLRGEDFSQLHIVGGGCQNTLLNQ
                     LCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDFRQVVSTTANLTTFTPNPDS
                     EIAHYVAQLHSTRQTKELCA"
     gene            31480..32739
                     /gene="rhaA"
                     /locus_tag="OQQ48_001157"
     CDS             31480..32739
                     /gene="rhaA"
                     /locus_tag="OQQ48_001157"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HCS1952003.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNFHAIYLESD
                     TPVSRDQIKPEHFKNWGEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISKKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDCDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKEILSQRG"
     gene            32822..33646
                     /gene="rhaD"
                     /locus_tag="OQQ48_001158"
     CDS             32822..33646
                     /gene="rhaD"
                     /locus_tag="OQQ48_001158"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HCS1952004.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YKDNVHAQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     STLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            33656..>33946
                     /gene="rhaM"
                     /locus_tag="OQQ48_001159"
     CDS             33656..>33946
                     /gene="rhaM"
                     /locus_tag="OQQ48_001159"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HCS1952005.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSEL"
BASE COUNT         8326 a         8852 c         8798 g         7970 t
ORIGIN      
        1 gaacaggcca cgccggtggg agcaccaatg cgcaccccgg ctgaaccctg aacaatgcta
       61 ccgccatact gcgtcatgtc gccctgacgc gccgccggtt ttccgctcat cgtaaaatcc
      121 ttttgaaata aataagaacc agaagcctag cgccagtcaa caacaggaaa tgataatgag
      181 ttaacacccc ggaatttttc agaatttata aaaggcaaaa ttcaggcaca aaaaaagcgc
      241 cgtgcggcgc ttttttcgga aatccggtct tatttgctgc ccgggatgtt gaaacgcttg
      301 ttgaagcggt caacacggcc accggtagca acatcacgct gtttgccagt gaagaacggg
      361 tggcacttgc tgcacacgtc gaggttcagg tcatgaccaa cagtggagcg gattttcatt
      421 acgttaccgc aagagcagct agcagtaatt tcttcgtatt tcgggtgaat atcttttttc
      481 atgggaaaac ctcggtttaa ggccgcgtcg ctcttccagc cctaacgcca gacaccacgc
      541 gatgttaaaa gtatagcttc aatacgatca tttcgtacga aggcgcgaaa tcatacagaa
      601 attaaccagc gtatgcaaac tgatccgcac tcttctacgg caatgtgtat actaacccac
      661 cgaatttcaa gtcaggatga tgctatgccc gttgcccacg ttgccttgcc cgttccgctt
      721 cctcgtacct ttgactatct gctgccagaa ggcatgacgg ttaaagccgg gtgtcgcgtg
      781 cgcgtgccgt ttggcaaaca gcaggagcgc atcggggttg tggtgtcagt tagcgatgtc
      841 agtgagctgc cgcttaacga gctgaaagcg gtagtggaag tgctggacgt tgagccggta
      901 tttactcact ccgtctggcg gttactgctc tgggcggcag attactatca tcatccgatt
      961 ggcgatgtgc tgtttcatgc cttgccgatt ttactgcgcc aggggcggcc tgcggcgaac
     1021 gcgccgatgt ggtactggtt tgccactgaa caaggccagg cggtggatct gaacagcctg
     1081 aaacgctccc ccaagcaaca acaggcgctg gcggcgttac ggcaaggcaa aatctggcgc
     1141 gaccaggtcg ccacgctcga atttaatgat gccgcgttgc aggcgctacg caaaaaaggt
     1201 ctgtgtgatt tagcaagtga aacaccagag tttagcgact ggcgaacgaa ctatgccgtt
     1261 tctggtgagc ggttgcgatt gaataccgaa caggccaccg ccgttggcgc aattcatagc
     1321 gcggcagata ctttttctgc ctggctgctg gcgggcgtta ccggttccgg taaaacggag
     1381 gtttatctca gtgtactgga aaacgtgctc gctcagggca aacaggcgct ggtgatggtg
     1441 ccggaaatcg gcctgacacc gcaaactatc gcccgttttc gtgaacgttt taatgccccc
     1501 gtggaagttc tgcattccgg cctgaacgac agcgagcggc tttctgcgtg gttgaaagcg
     1561 aaaaatggcg aggcggcgat tgtgatcggc acccgctccg cgctgtttac gccgtttaaa
     1621 aatctcggcg tgattgtcat tgatgaagag cacgacagct cctacaagca gcaggaaggc
     1681 tggcgctatc atgcccgcga cctggcggtg tatcgtgcgc acagcgagca aatcccgatt
     1741 attcttggct ccgcaacgcc cgcgctggaa acgttatgca acgtccagca gaaaaaatac
     1801 cgcctgctgc gcctgacccg tcgggcaggg aatgcttgtc cggcaattca acatgtgctg
     1861 gatttaaaag gtcagaaggt gcaggcaggt ctggctccgg cgttaatcac ccgtatgcgc
     1921 cagcatttac aggccaacaa tcaggttatc ctctttctta atcgtcgggg ttttgcgcct
     1981 gcgctgctgt gccacgactg cggctggata gccgaatgcc cacgttgcga tcactactac
     2041 acgctgcatc aggcgcaaca gcatttgcgc tgtcaccact gtgacagtca gcgtccggtg
     2101 ccgcgccagt gcccttcctg cggttccacg cacctggtcc ccgtggggct gggcaccgaa
     2161 cagcttgaac agacgcttgc gccgatgttc cccggcgtgc ccatttcacg tatcgaccgc
     2221 gataccacca gccgcaaagg ggcactggaa cagcaactgg cggaagtgca tcgcggcggc
     2281 gcgaggattt tgatcggcac acaaatgctg gcgaaaggtc accactttcc ggatgtgacg
     2341 ctggtggcgt tactggatgt ggacggcgcg ctgttttctg ccgattttcg ctcggcagaa
     2401 cgtttcgccc agctttacac ccaggtcgcc ggtcgtgccg ggcgtgcggg taaacagggc
     2461 gaagtggtgc tgcaaacgca ccatccggaa catcctctgt tgcaaacgtt gctctataaa
     2521 ggctacgacg cctttgccga acaggcgctg gctgagcgac gcatgatgca gctgccgccg
     2581 tggaccagcc atgtgattgt gcgtgcggaa gatcataaca atcagcacgc gccgctgttc
     2641 ctgcaacaac tgcgtaatct gatcctttcc agcccgctgg cagacgacaa actgtggatt
     2701 ctcggcccgg ttccggctct ggcacctaag cgtggtggcc gctggcgctg gcagatcttg
     2761 ttgcagcacc cttcccgcgt gcgcttgcaa cacatcatta gtggtacgct ggcgcttatt
     2821 aatacaatac cggattcccg caaggtgaaa tgggtgttgg atgtcgatcc catcgaaggc
     2881 taatgcgcaa acgttgcgag gcggatcgaa aaattcaata ttcatcacac ttttcatgaa
     2941 aattctgtaa ccgttttcac gcgctatctg ctaaaaatgt tgccgatgtg aagtaaacat
     3001 ggatgtagta cgcctgacgt accaggcgag gagtgagtgt gaaagcgaag aagcaggaaa
     3061 ctgccgcgac catgaaagac gttgccctca aggcaaaagt ctctacagcg actgtctccc
     3121 gagcattaat gaatcccgat aaagtctccc aggccacccg taatcgggtt gaaaaagcgg
     3181 cccgggaagt gggttattta ccgcagccta tggggcgcaa cgtcaagcgt aatgaatccc
     3241 gcaccattct ggtgattgtc ccggatatct gcgatccctt ctttagcgaa attattcgcg
     3301 gtatcgaagt tacggcggca aatcacggat atctggtgct gattggcgac tgtgcgcatc
     3361 aaaatcagca ggaaaaaacc tttatcgatt tgatcatcac caagcaaatt gatggcatgt
     3421 tgctgctggg ttcaaggctg ccgtttgatg ccagcattga ggaacagcgt aatctgccgc
     3481 cgatggtgat ggcgaacgaa tttgcaccgg aactggagct gcctaccgtt catatcgaca
     3541 atctgaccgc cgcatttgat gcagtaaatt atttatatga gcaagggcat aaacggattg
     3601 gctgtatagc cggtcccgaa gagatgccac tgtgtcacta ccgcctgcaa ggctatgttc
     3661 aggcgctgcg tcgctgcgga attatggttg atccacaata catcgcccgt ggcgacttca
     3721 ccttcgaagc cggaagcaaa gcaatgcaac agctgcttga tcttccacaa ccgcctactg
     3781 cagtcttctg ccatagcgat gtgatggcgc tcggcgcact ttctcaggca aaacgccagg
     3841 ggctgaaagt cccggaagac ctttccataa tcggttttga taacatcgac ctgacgcaat
     3901 tttgtgatcc gccgctgaca accatcgcgc agccgcgtta cgaaatcggt cgggaagcta
     3961 tgctgttatt gcttgatcaa atgcaggggc aacacgttgg cagtggctct cgtttaatgg
     4021 actgcgaact tatcatccgg ggatcaacac gcgcgttacc ttaaagtaaa cctttaagac
     4081 tgccgtgact ggtcaaaggc ccgccgctta agtaacatgg cgggctgacg aacgaataaa
     4141 tacagcgaaa cgatagtggc acaacgagat tatgtacgcc gcagccaacc ggcaccttcg
     4201 cggcgaaaaa agagcacctc acggaaaaag caacgaaatc tgcctgcggt ttctcccgct
     4261 atggtcgcta ttgctgccgc cgttcttgtg acctttatcg gtggtctgta cttcattacg
     4321 catcacaaga aagaagagtc cgagacgctg caaagccaga aagtgaccgg aaacggacta
     4381 ccaccaaaac cagaagaacg ctggcgctac attaaagagc tggaaagtcg ccagccgggt
     4441 gtgcgtgcgc ccacagaacc ttctgccggt ggtgaagtga aaacgccgga gcaactgaca
     4501 ccagaacaac gtcagcttct tgaacaaatg caggctgata tgcgccagca gccaacgcag
     4561 ctggttgagg ttccgtggaa tgaacagacg ccggaacagc gtcagcaaac gctacaacgc
     4621 cagcgtcagg cgcagcaatt agcggagcag caacgcctgg ctcagcagtc gcgtacgact
     4681 gaacaaagct ggcagcagca gacgcgtacg tcgcaagccg cgccggtaca ggcgcaaccg
     4741 cgccagtcta aaccagcttc tactcagcag ccgtaccagg atctgctgca aacccctgcg
     4801 cacacgaccg cgcagtcgaa gccgcagcaa gctgcaccgg ttgctcgtgc cgctgacgcg
     4861 ccaaaaccga cggcggagaa aaaagacgaa cgccgctgga tggtgcagtg cggttcgttc
     4921 agaggcgcgg aacaggcaga gacggtacgt gctcagctgg cgttcgaagg atttgactcg
     4981 aaaatcacca ccaacaatgg ctggaatcgt gtggtcattg gcccggtgaa aggcaaagag
     5041 aacgcagaca gcaccctcaa tcggttgaag atggcgggtc atacaaactg cattcggctc
     5101 gccgccgggg gttgaaaccc tcaaaatccc ccccatctat aattgcatta tgcctcgtac
     5161 ttttgtacgg ggtttgtact ctgtattcgt aaccaagggg tcagctcgtg acaactatag
     5221 taagcgtacg ccgtaacggc catgtggtca tcgctggtga tggtcaggcc acgttgggca
     5281 ataccgtaat gaaaggcaac gtgaaaaagg tccgccgtct gtacaacgac aaagtcatcg
     5341 cgggctttgc gggcggtact gcggatgctt ttacgctgtt cgaactgttt gaacgtaaac
     5401 tggaaatgca tcagggccat ctggttaaag ccgccgttga gctggcaaaa gactggcgta
     5461 ccgatcgcat gctgcgcaaa cttgaagcac tgctggcagt cgcggatgaa actgcatcgc
     5521 ttatcatcac cggtaacggt gacgtggtgc agccagaaaa cgatcttatt gctatcggct
     5581 ccggcggccc ttacgcccag gctgcggcgc gcgcgctgtt agaaaacact gaacttagcg
     5641 cccgtgaaat tgctgaaaag gcgttggata ttgcaggcga catttgcatc tataccaacc
     5701 atttccacac catcgaagaa ttaagctaca aagcgtaagg atctcccatg tctgaaatga
     5761 ccccacgcga aatcgtcagc gaactggata agcacatcat cggccaggac aacgccaagc
     5821 gttctgtggc gattgctctg cgtaaccgct ggcgtcgcat gcagctcaac gaagagctgc
     5881 gccatgaagt gaccccgaaa aatatcctga tgatcggccc gaccggtgtc ggtaaaactg
     5941 aaatcgcccg tcgtctggct aagctggcga atgcgccgtt catcaaagtt gaagcgacca
     6001 aattcaccga agtgggctat gtcggtaagg aagtggattc tattattcgc gatctgaccg
     6061 atgccgccgt gaaaatggta cgcgtccagg ctatcgagaa aaaccgttat cgcgctgaag
     6121 aactggcaga agaacgtatt ctcgacgtgc tgatcccacc tgctaaaaac aactggggac
     6181 agaccgaaca gcagcaggaa ccgtccgctg ctcgtcaggc attccgcaaa aaactgcgtg
     6241 aaggccagct tgatgacaaa gaaatcgaga tcgatcttgc cgcagcaccg atgggcgttg
     6301 aaattatggc tcctccaggc atggaagaga tgaccagcca gctgcagtcc atgttccaga
     6361 acctgggcgg ccagaagcaa aaagcgcgta agctgaaaat caaagacgcc atgaagctgc
     6421 tgattgaaga agaagcggcg aaactggtga acccggaaga gctgaagcaa gacgctatcg
     6481 acgctgttga gcagcacggg atcgtgttta tcgacgaaat cgacaaaatc tgtaagcgcg
     6541 gcgagtcttc cggtccggat gtttctcgtg aaggcgttca gcgtgacctg ctgccgctgg
     6601 tagaaggttg caccgtttcc accaaacacg ggatggtcaa aactgaccac attctgttta
     6661 tcgcttctgg cgcgttccag attgcgaaac cgtctgacct aatcccggaa ctgcaaggtc
     6721 gtctgccaat ccgcgttgaa ctgcaggcac tgaccaccag cgacttcgag cgtattctga
     6781 ccgagccgaa tgcctctatc accgtgcagt acaaagcact gatggcgact gaaggcgtaa
     6841 atatcgagtt taccgactcc ggtattaaac gcatcgcgga agcggcatgg caggtgaacg
     6901 aatctaccga aaacatcggt gctcgtcgtt tacacactgt tctggagcgt ttaatggaag
     6961 agatttccta cgacgccagc gatttaagcg gtcaaaatat cactattgac gcagattatg
     7021 tgagcaaaca tctggatgcg ttggtggcag atgaagatct gagccgtttt atcctataat
     7081 cgcgttcaat cattttcatc attgtttgat ggggctgaaa ggccccattt ttattggcgc
     7141 gtattatgac tgaacaacaa attagccgaa ctcaggcgtg gctggaaagt ttacgaccta
     7201 aaaccctccc cctcgccttt gctgcaatta tcgtcgggac ggcgctggca tggtggcaag
     7261 gtcattttga tccgctggtc gccctgctgg cactgattac cgccgggcta ttacagatcc
     7321 tttctaacct cgccaatgat tacggcgatg cggtaaaagg cagcgataga cctgaccgca
     7381 ttgggccgct acgcggcatg caaaaagggg tcattaccca gcaagagatg aaacgggcgc
     7441 tcattattac cgtcgtgctc atctgtctct ccgggctggc actggttgca gtggcgtgcc
     7501 atacgctggc tgattttgtc ggtttcctga ttcttggcgg gttgtcgatc attgccgcta
     7561 tcacctacac cgtgggcaat cgtccttatg gttatatcgg tctgggggat atttccgtac
     7621 tggttttctt tggctggtta agcgtcatgg ggagctggta tttacaggct catacattga
     7681 ttccggcact gatccttccg gcgaccgcat gcggcctgct ggcaacggca gtactgaaca
     7741 ttaataacct gcgtgatatc aatagcgacc gagaaaacgg caaaaacacg ctggtggtgc
     7801 gcttaggtga agtgaacgcg cgtcgttatc atgcctgcct gctgatgggc tctctggtat
     7861 gtctggcgct gtttaatctc ttttcgctgc atagcctgtg gggctggctg ttcctgctgg
     7921 cggcaccgtt gctggtgaag caagcccgtt atgtgatgcg ggaaatggac ccggtggcga
     7981 tgcgaccgat gctggaacgc actgtcaagg gggcgttact gactaacctg ctgtttattt
     8041 tagggatatt cctaagccag tgggcagcat aactgacaaa tatcaattaa caattgatga
     8101 ttttgccaac agcccacata gcgcgatata ctgaaaattc tcgcagcaac tgaatgttaa
     8161 gcctatgaaa tacgatactt ccgagctttg tgacatctat caagaagatg ttaacgtcgt
     8221 ggaaccgctg ttctccaact ttggcggacg ggcgtcgttt ggcggacaaa taatcacggt
     8281 aaaatgtttc gaggacaacg ggttgctgta cgatctgctc gaacagaatg gccgtggtcg
     8341 tgttcttgtc gtcgatggcg gtggttctgt tcgtcgcgca ctggtcgatg ctgaactggc
     8401 gcgtctggca gtacaaaatg aatgggaagg tctggttatt tacggcgcgg tgcgtcaggt
     8461 agatgacctg gaagagttgg atatcggcat ccaggcgatg gcggcaattc cggttggtgc
     8521 cgctggcgaa ggcattggcg aaagcgatgt ccgcgtcaat tttggcggtg taaccttctt
     8581 ctccggcgac catctttatg ccgacaatac cgggattatt ctttcagaag atccgctgga
     8641 tattgaatga taagaaaggc accgcaaggt gccttttttc tgcgttacct gttggcctac
     8701 acagtaaaga aattacgcga aagatgaagc gtaatcagac ctcttccatg cgacccagca
     8761 gggcctgcag acgttcctgc cagccgttct gctgttcttt cagatggttg ttctcacgct
     8821 ccagctcttc gcgctgatgc tgggcatttt gaacttcctg cgacagtgag ttgttttttt
     8881 ctttcagctc ttcgatttcc atctgcaaca gagtgatggt atcaatcgcc tgctgtactt
     8941 ttgcttccag tttctcaaac acttctaatg acattgtcat acctctcctg aattgcaagg
     9001 cgttgatgga taaaaaatcc tcgtcccgat taccggtgac gccttaataa atacgagcgc
     9061 actttagtta gctccgattg tatgaagccg cgccatcgct gtccagcggc acgccttgca
     9121 gattacggtt tgccacactt ttcatccttc tcctggtgac ataatccaca tcaatcgaaa
     9181 atgttaataa atttgttgcg cgaatgatct aacaaacatg catcatgtac aatcagatgg
     9241 aataaatggc gcgataacgc tcattttatg acgaagcaca cacattttaa gttcgatatt
     9301 tctcgttttt gctcgttaac gataagttaa cagcatgcct acaagcatcg tggaggtccg
     9361 tgactttcac gcatacaaca aacattaact cttcaggatc cgattatgag tcaaacatca
     9421 accttgaaag gccagtgcat tgctgaattc ctcggtaccg ggttgttgat ttttttcggt
     9481 gtgggttgcg ttgcagcact aaaagtcgct ggtgcgtctt ttggtcagtg ggaaatcagt
     9541 gtcatttggg gactgggggt ggcaatggct atctacctga ccgcaggggt ttccggcgcg
     9601 catcttaatc ccgctgttac cattgcattg tggctgtttg cctgtttcga caagcgcaaa
     9661 gttattcctt ttatcgtttc acaagttgcc ggcgctttct gtgctgcggc tttagtttac
     9721 gggctttact acaatttatt tttcgacttc gagcagactc atcacattgt tcgcggcagc
     9781 gttgaaagtg ttgatctggc aggcactttc tctacttacc ctaatcctca tatcaatttt
     9841 gtgcaggctt tcgcagttga gatggtgatt accgctattc tgatggggct gatcctggcg
     9901 ttaacggacg atggcaacgg tgtaccacgc ggccctttgg ctcccttgct gattggtcta
     9961 ctgattgcgg tcattggcgc atctatgggc ccattgacgg gttttgccat gaacccagcg
    10021 cgtgacttcg gtccgaaagt ctttgcctgg ctggcgggct ggggcaatgt cgcctttacc
    10081 ggcggcagag acattcctta cttcctggtg ccgctttttg gccctatcgt tggcgcgatt
    10141 gtaggtgcat ttgcctaccg caaactgatt ggtcgccatt tgccttgcga tatctgtgtt
    10201 gtgaaagaaa aggaaaccac aactccttca gaacaaaaag cttcgctgta atatgactac
    10261 gggacaatta aacatgactg aaaaaaaata tatcgttgcg ctcgaccagg gcaccaccag
    10321 ctcccgcgcg gtcgtaatgg atcacgatgc caatatcatt agcgtgtcgc agcgcgaatt
    10381 tgagcaaatc tacccaaaac caggttgggt agaacacgac ccaatggaaa tctgggccac
    10441 ccaaagctcc acgctggtag aagtgctggc gaaagccgat atcagttccg atcaaattgc
    10501 agctatcggt attacgaacc agcgtgaaac cactattgtc tgggaaaaag aaaccggcaa
    10561 gcctatctat aacgccattg tctggcagtg ccgtcgtacc gcagaaatct gcgagcattt
    10621 aaaacgtgac ggtttagaag attatatccg cagcaatacc ggtctggtga ttgacccgta
    10681 cttttctggc accaaagtga agtggatcct cgaccatgtg gaaggctctc gcgagcgtgc
    10741 acgtcgtggt gaattgctgt ttggtacggt tgatacgtgg cttatctgga aaatgactca
    10801 gggccgtgtc catgtgaccg attacaccaa cgcctctcgt accatgttgt tcaacatcca
    10861 taccctggac tgggacgaca aaatgctgga agtgctggat attccgcgcg agatgctgcc
    10921 agaagtgcgt cgttcttccg aagtatacgg tcagactaac attggcggca aaggcggcac
    10981 gcgtattcca atctccggga tcgccggtga ccagcaggcc gcgctgtttg gtcagttgtg
    11041 cgtgaaagaa gggatggcga agaacaccta tggcactggc tgctttatgc tgatgaacac
    11101 tggcgagaaa gcggtgaaat cagaaaacgg cctgctgacc accatcgcct gcggcccgac
    11161 tggcgaagtg aactatgcgt tggaaggtgc ggtgtttatg gcaggcgcat ccattcagtg
    11221 gctgcgcgat gagatgaagt tgattaacga cgcctacgat tccgaatatt tcgccaccaa
    11281 agtgcaaaac accaatggtg tgtatgtggt tccggcattt accgggctgg gtgcgccgta
    11341 ctgggacccg tatgcgcgcg gggcgatttt cggtctaact cgtggggtga acgctaacca
    11401 cattatacgc gcgacgctgg agtctattgc ttatcagacg cgtgacgtgc tggaagcgat
    11461 gcaggccgac tctggtatcc gtctgcacgc cctgcgcgtg gatggtggcg cagtagcaaa
    11521 caatttcctg atgcagttcc agtccgatat tctcggcacc cgcgttgagc gcccggaagt
    11581 gcgcgaagtc accgcattgg gtgcggccta tctcgcaggc ctggcggttg gcttctggca
    11641 gaacctcgac gagctgcaag agaaagcggt gattgagcgc gagttccgtc caggcatcga
    11701 aaccactgag cgtaattacc gttacgcagg ctggaaaaaa gcggtcaaac gcgcgatggc
    11761 gtgggaagaa cacgacgagt aatgtaaatg ccggatgaag cgtttatgcc gcatccggta
    11821 gtcctgaaac gtgcgggggc aaccccgcac acatcaataa tcccaccctt cccctgtgct
    11881 acacttcgcg ccattcctta ctgcttagag tttgctatga gacgagaact tgccatcgaa
    11941 ttttcccgcg tcaccgaatc tgcggcgctg gctggctaca aatggttagg acgcggcgat
    12001 aaaaacaccg cggacggcgc ggcggtaaac gccatgcgta ttatgctcaa ccaggtcaac
    12061 attgacggca ccatcgtcat tggtgaaggt gaaatcgacg aagcaccgat gctctacatt
    12121 ggtgaaaaag tcggtactgg tcgcggcgac gcggtagata ttgctgttga tccgattgaa
    12181 ggcacgcgca tgacggcgat gggccaggct aacgcgctgg cggtgctggc agtaggcgat
    12241 aaaggctgct tcctcaatgc gccggatatg tatatggaga agctgattgt cgggccggga
    12301 gccaaaggca ccattgatct gaacctgccg ctggcggata acctgcgcaa tgtagcggcg
    12361 gtgctcggca aaccgttgag cgaactgacg gtaacgattc tagctaaacc acgccacgat
    12421 gccgttatcg ctgaaatgca gcaactcggc gtacgcgtat ttgctattcc ggatggcgac
    12481 gttgcggcct caattctcac ctgtatgcca gacagcgaag ttgacgtgct gtacggtatt
    12541 ggtggcgcgc cggaaggcgt agtttctgcg gcggtgatcc gcgcattaga tggcgacatg
    12601 aacggtcgtc tgctggcgcg tcatgacgtc aaaggcgaca acgaagagaa tcgtcgcatt
    12661 ggcgagcagg agctggcacg ctgcaaagcg atgggcatcg aagccggtaa agtattgcgc
    12721 ctgggcgata tggcgcgcag cgataacgtc atcttctctg ccaccggtat taccaaaggc
    12781 gatctgctgg aaggcattag ccgcaaaggc aatatcgcga ctaccgaaac gctgctgatc
    12841 cgcggcaagt cacgcaccat tcgccgcatt cagtccatcc actatctgga tcgcaaagac
    12901 ccggaaatgc aggtgcacat cctctgattg atttgatcga ttgagccttc cagtccttcg
    12961 ggactggaat ttttttgttc ggagaacgaa gataaggcaa gtcaatcaaa acaggagaaa
    13021 aacatggctg attgggtaac aggcaaagtc actaaagtgc agaactggac cgacgccctg
    13081 tttagtctca ccgttcacgc ccccgtgctt ccgtttaccg ccgggcaatt caccaagctt
    13141 ggccttgaaa tcgacggcga acgcgtccag cgcgcctact cctatgttaa ctcgcccgat
    13201 aatcccgatc tggagtttta cctggtcacc gtccccgatg gcaaattaag cccacgattg
    13261 gcggcactga aaccaggcga tgaagtgcag gtggttagcg aagcagctgg cttctttgtt
    13321 ctggatgaag taccagattg cgaaacgcta tggatgctgg caaccggtac agcgattggc
    13381 ccttatttat cgattctgca actaggcaaa gatttagatc gcttcaaaaa tctggtcctg
    13441 gttcacgccg cacgttatgc cgccgactta agctatttgc cactgatgca agaactggaa
    13501 aaacgctacg aaggcaaact gcgcattcag acggtggtca gtcgggaaac ggcagcgggg
    13561 tcgctcaccg gacggatacc ggcattaatt gaaagtgggg aactggaaag cgcgattggc
    13621 ctgccgatga ataaagaaac cagccatgtg atgctgtgcg gcaatccaca gatggtgcgc
    13681 gatacacaac agttgctgaa agagacccgg cagatgacga aacatttacg tcgccgaccg
    13741 ggccatatga cagcggagca ttactggtaa gcggttactt atcgataaac ggcacgatga
    13801 gcaaatccgc actcatctta ttgatcatcc cgcgatatgc tggcatcaaa cggttgataa
    13861 atgagtgatg atgaccacag acaaggaggt cgcactgctc tttttgcata atttccagca
    13921 gtgtttccgg catttctccg cgttcaatac gcagttttgt cttcggccat tgaatatttt
    13981 tcgtcagttt atacagcttg ttatccgact tattcttcaa caattgaaga atatcttctg
    14041 ttgcagggaa gtagataccc ggatacaact cgcttaagcc atcatcaata tgaattaacg
    14101 tcaggtgagc gtcattatgt ctggcgagct ccagagcttt attcaccagt aaggcatctt
    14161 cttcattccc ggaaattgcc acgccaatgt gtttataagc catgtttaac tccttctaaa
    14221 gcaactccat caagctaacg aacgcagtga taactcaaaa ataatcatct cggcctggca
    14281 tgagaaagtg aaggccgcat caagctcaac ttgcccctcc agatccttaa tcttgtaggc
    14341 cacgctggcc ccttcatctg cagaggatgc agcggcggca agctctttca gtttggtcat
    14401 aaaagactct gcttcttcgc gggtggcaaa aaagcgcgaa aacttactgg tacagttgtc
    14461 gttatcaatc accgtaccga tatcaatcgc acatccttta gtactgcatt tatctacgac
    14521 atctttcata gggcacctct gtatattcgc ctctctgtcg attacgactt aagaataatg
    14581 cgccgttaag ggaagaacaa acaagtaatg atcttatgga ttaccatttt ttttgatatt
    14641 gcttaaggtg aagagttcag aaggattttc cgggtattgt catttgcatc gtggaaataa
    14701 caatcccgga aaaaaatagc agtaagagat atattaaagg ttaattactt gatttattgt
    14761 cggctttata cttcacatcc tgagtatctt taccatattt attttcgcct tgtgtgccaa
    14821 caaacgcacc aagatcgata agcatcatca ccagaatcaa cgtcgggaca aaacacccca
    14881 ccgcccattg ccagacaccc ggtaaaatcg cccagttacc cgccagcagc atccacgcca
    14941 caatcatcag aaatgcccat gcgccggaac gcccgcgatc atgcaagcgc ttaacagtta
    15001 ctgccgctgt tggccaaagc aagcacacaa ggcaaaacgc cgcggtctga atatcgagta
    15061 aatttttacc cgccagtgag aaaagcacca gcatgcctgc gaaccacagg cctatccaaa
    15121 tccagaaatc acggcgtcca atacgccctt taaatgagaa taaccattgc tgtatggtca
    15181 tgtaagttcc ttgatggttg tcttttccag gattctaccc ttttgacaag ggcgacagat
    15241 atcgttttaa tcggagccag tcataacaaa aggtactgtc aatgaagcca gggtgtacgc
    15301 tgttttttct cttatgttct gcattaaccg ttacaacaac ggcgcatgca caaacaccag
    15361 atacggcaac gaccgcgcct tatctgctgg ctggagcccc tactttcgat ctctccatca
    15421 gccagtttcg agaagacttt aacagccaga atcctagcct gccactgaac gaatttcgtg
    15481 ccatcgacag cagtcccgac aaagccaatc tcactcgtgc tgcaagtaaa attaacgaga
    15541 acttgtatgc ttctacagcg ctggagcgcg gtaccttaaa aatcaaaagc attcaaatga
    15601 cctggctacc catccagggg ccagagcaaa aagcagcgaa agcgaaagct caggaataca
    15661 tggcagcagt gatccgcaca ctcaccccat taatgaccaa aacacaaagc cagaaaaaac
    15721 tgcagtcgct gctaacggcg gggaaaaaca aacgttatta caccgagaca gaaggtgcac
    15781 tgcgttatgt tgtcgcggac aacggcgaaa aggggctgac cttcgctgtt gaaccgatta
    15841 agctggcgct atctgaatcg cttgaaggtt tgaataaatg acaaaaagca aagcctttgt
    15901 gccgatgaat ctctatactg tttcacagac ctgctgccct gcggggcggc catcttcctt
    15961 tattcgctta taagcgtgga gaattaaaat gcgacatcct ttagtgatgg gtaactggaa
    16021 actgaacggc agccgccaca tggttcacga gctggtttct aacctgcgta aagagctggc
    16081 aggtgttgct ggctgtgcgg ttgcaatcgc accaccggaa atgtacatcg atatggcgaa
    16141 gcgcgaagct gaaggcagcc acatcatgct gggtgcgcaa aacgtggacc tgaacctgtc
    16201 cggcgcattc accggtgaaa cctctgctgc tatgctgaaa gacatcggcg cacagtacat
    16261 catcatcggc cactctgaac gtcgtactta ccacaaagag tctgacgaac tgatcgcgaa
    16321 aaaattcgcg gtgctgaaag agcagggtct gactccggtt ctgtgcatcg gtgaaaccga
    16381 agctgaaaac gaagcgggca aaactgaaga agtttgcgca cgtcagatcg acgcggtact
    16441 gaaaactcag ggtgctgcgg cattcgaagg tgcggttatc gcttacgaac ctgtatgggc
    16501 aatcggtact ggcaaatctg caactccggc tcaggcacag gctgttcaca aattcatccg
    16561 tgaccacatc gctaaagttg acgctaacat cgctgaacaa gtgatcattc agtacggcgg
    16621 ctctgtaaac gcgtctaacg ctgcagaact gtttgctcag ccagacatcg acggcgcgct
    16681 ggttggcggt gcgtctctga aagctgacgc tttcgcagta atcgttaaag ctgcagaagc
    16741 ggctaaacag gcttaagtct gacaggtgcc ggatttcata tccggcactt actttcctta
    16801 actcttcgcc ttaacgcaaa atctcacact gatgatcctg aatttcctcg gctgaagcac
    16861 ggttaagcgt cagtagattt cgttgtgtcg ccagcaatac aaatgagtta tcactctgcc
    16921 gtaccatcgc cagcccgtag cttcccatat gttcccgcgc ctcaggtact tcttctgcca
    16981 gcatcataaa tgggctgcgt tgtaccagtt cgctttccgt tacccgacgc gccaggtatt
    17041 catgcccgcg caaaccacct ggcagtggca accagcggct gctgatgttc gccagattgt
    17101 tatccagttg ttcgcgcaca tcaggacgaa tacaagagat atgaatatga aaatggtttt
    17161 gcgtacgccc ggtgcgggag ttgatcgcca aagaaaccgc gcgatcggga accggctggc
    17221 cgtacttttt gctcataaaa tcacgcgcct gccaggccaa ccaaaagaag ttcggcgttg
    17281 aaggatcggt caacaaagga ctttcagtac cgttaatacg atacgttggc atcaacagat
    17341 attgcagtgg gccattaaga tcttttaaaa ccacgtatcc ggcattgggt ttgacttccg
    17401 cacatggcga aggattttga ttttgctgct gattgggcaa acattcctca aggacaatct
    17461 tacgtaatgt atccgactct tcaccggtta atttccagta accaataccg gcagccacaa
    17521 cggcgataac tatcatcacc aaaaaaagaa gacccgcttt tttcatcttt ttttccctgt
    17581 acctcaaaga gatgcaaggg taacgcaaaa tcgtgacaaa taaaaaaccc ggtcgcgaga
    17641 aaccaccggg tttgataatt atcctgggga aatcagcgtt tgctgatctg atcgaacgta
    17701 ccgccgttag caaaatgctc tttttgcgct ttcgtccagc cgccgaactc ttcatcaatg
    17761 gtgaataact tcagctttgg aaacgcattt tcgtactttt tcgctacttc agcgtcgcgc
    17821 ggacggtagt agtttttcgc ggcaatttcc tgaccttctg gcgagtagag atatttcagg
    17881 taggcttccg ccacctcttt ggtgcctttt ttctcgacca ctttatcgac caccgacacg
    17941 gttggctctg cgaggataga ctcactcggc gtgacgattt cgaatttatc tttccccagt
    18001 tcattcgctg ccagcagagc ttcgttttcc caggcgatca gtacatcgcc aatcccgcgc
    18061 tcgacaaagg tgttagtgga gccgcgcgcg ccagaatcca gaacttcgac gtttttatac
    18121 agtgcccgaa caaaatcctg tgcttttgcc tgatcgttgt tgttgtgatg cagcgcgtag
    18181 ccccaggcag ccaagtagtt ccagcgtgcg ccaccagagc ttttcggatt aggcgtgatc
    18241 accgaaacac ccggtttaat cagatcgttc cagtcatgga tctgcttcgg attgccctta
    18301 cgcaccagga aaacaatggt ggaagtgtac ggtgcggagt tatccggcag acgtttgatc
    18361 cactctttat caatccgccc gcgttccgca attgcgtcca cgtcataggc cagagccagc
    18421 gtgacaacat cagcttcaat accgttgatt accgacgtcg cttgtttacc tgaaccaccg
    18481 tgtgactgac gcatcaccac gttatcacca gtttgctgtt tccagtgggc gctgaatgcc
    18541 ttgttgtact gttcgtacaa ttcgcgcgtt ggatcatatg aaacgttaag aagctgaata
    18601 tcctttgcca taacgctggt tgccgccagc aaaaatgtta accctacgcc ccacttgttc
    18661 atcgcccgac tctcttatgt tgtgttgtga tgagcaaagc gtgccagaag gttaaccaaa
    18721 cattaaagaa taaaaaaaga ttggctataa cttgcgggta tatgttgagg gattaaaaag
    18781 gcggaagaaa tccgcctcat attgctgaca aagtgcgctt tgtccatgcc ggatgcggcg
    18841 cgaacgcctt atccggccta caaaagtttg caaattcaat aaattgcaga attcatgtag
    18901 gcctgataag cgaagcgcat caggcatttt tgcttctgtc atcggtttca ggctaaagga
    18961 atctgccttt ttccgaaatc attaatacag ttttttcgcg cagtccagcc agtcaccttt
    19021 gaacggacgc ttcatgtttt cgatagcgtc gatgatgtcg tggtgaacca gctgttcgtt
    19081 ctggatacct acacaacgac cgccgtaacc tgccagcagc agatcgatag cgtaagcgcc
    19141 catacgggaa gccagaatac ggtcgtaagg caccggagaa ccaccgcgct ggatgtggcc
    19201 cagcacagtt gcgcgggttt cacgacctgt ttctttctcg atgaaatgcg ccagttcgtc
    19261 aacatcacac atatgttcgg taatcgccac gatcgcgtgt tttttacctt tcgcgatacc
    19321 cgctttgatt tcgtttacca ggtcttcacg gctgaattca acttccggaa ccacaacgaa
    19381 ttcacagcca ccggcaatgg ccgcagccaa cgtcagatcg ccacaataac ggcccatcac
    19441 ttccaccacg gaaatacgct ggtgagaaga agaggtgtca cgcagacggt cgatcgcttc
    19501 tacaacggtg ctcagcgcag tgaagaaacc gatagtgtag tcagtgcctt tgatgtcgtt
    19561 gtcgatagtg cccggcagac cgatgcacgg gaagcccatt tcggtcagac gcattgaacc
    19621 catgtaggaa ccgtcaccgc cgataaccac cagcgcgtcg ataccacgtt ttttcaggtt
    19681 ttcgatagcc acggcgcgga tgttctcgtc gcggaattcc gggaaacgcg cagaaccgag
    19741 gaacgtaccg ccacggttga tcatgtcaga cacgctgtaa cggtctagct gtaccatacg
    19801 gtcttcatac agacccagat agccgtcata aatacccatt acttccagac cttctgtcag
    19861 cgcagaacga acaaccccgc gaattgcggc gttcatgcct ggcgcatcac cgccgcttgt
    19921 caacacaccg attttcttaa tcatgactac ctctgaactt tggaatgcaa aatgaaatct
    19981 gttgccggaa gtcttcttgc acatcgaagt gatccaacga atgtgcaaat agtataacaa
    20041 tcacttcctg ctgaattgat tcaggtcagg ccaaatggcg gtattttata cacaaaatgc
    20101 gggtctggct ctcttttata ctgattatga aagcatagac cgtttaccct ccctgggtac
    20161 gacggaacag gggtcctgat ggataattac atccgatccc ggaaaacgcc gtaaaatagc
    20221 ctgctctacc tgatccgcca ccatatgtgc ctgaaccaaa ggcagagagt cttccatttc
    20281 caaatgaatc tgaataaagc gggtcggccc tgactgccgc gtgcgaagat cgtgagcgcc
    20341 gctaacaccc ggccaggaag tcacgatatc aataatttct tgccgttcct catcaggcaa
    20401 tgcgcgatcc agtaatgact gtaccgcctc atatcccatg cgtaacgcgc tatataaaat
    20461 atagatgccg attcccaatg caaacagagc atcggcgcga tgccagccgt accaggacaa
    20521 ccccagcgcc agcagaattg cgccgttcat cataacatca gactggtaat gtagcatatc
    20581 agcccgcacc gcctggcttt gcgtccggcg caccacccaa cgctgaaacg agacaaggat
    20641 aatcgtacaa attagcgcca caattgtcac gataaccccg acgcctggat ctatcatcgg
    20701 tgttggagat atcagatgtt gaatacccgt caaaaacagg aatagtgccg aaccggagat
    20761 aaacatactt tgcgccagcg ccgcgaggga ctctgcttta ccgtgaccaa acgagtgatt
    20821 atcgtcggca ggttgcaggg aatatcgcac caccagtaaa ttcgtcaacg acgcgccgat
    20881 atccaccagc gaatccacca gcgcggcgag aatactcacc gacccggtat accaccatgc
    20941 aaaaatttta atcagcaata gcaacgaagc catcgccgtc gcagcaatcg ccgcccgact
    21001 gaccagccgt ccataagatt gattcataaa tactcccgct atcaactgac gctagtataa
    21061 cggaagcaaa tcatctgcaa tgcattaagc agcaggcaaa ttgaggataa aaaaaacccc
    21121 acatcatgtg ggggaagaca gggatggtgt ctatggcaag gaaaacaggg tttactactg
    21181 ggaacgtgag ttgctactac tcaatagctt caacgatgaa cttttttgcc attgcgtcac
    21241 gtcacgcaac tgctccattc gttgttgatg tttctcgttt aaaaccgctt gctgctccgg
    21301 cgttaacagg cgatacattt ggttgcggac ttttgccatc tcaacctgac gagcaatttg
    21361 ctcattcgcc attttttctg cctgtgcgcg cacagcgttt tcatcaaaat tttctgcggt
    21421 gacaaggcga tgcattgtct ccagttcgct aacattaaca ggaggctgtt cgtgccgggc
    21481 ctgttgcata agatctcgca tctgctgacg ctgatgttcg gttaaactta tgccgtcgaa
    21541 catatggctc tgcgtactgc gctgcgtact gcgctgcgta ctgcgctgcg taagttcttc
    21601 acccggatgc cagttatcgc ctgaaccgac ttcagcagcg tggcttaatg aactgactgc
    21661 cagcgttgaa gccatgacgg cagcggtaac tatgcgcatc atttgctccc aaaatctttc
    21721 tgtcgcgatt caacgataga gagtttacga ttcaggctgc aaacatgcgt cagggggtgt
    21781 aaaacaacgt aaagtcatgg attagcgacg tctgatgacg taatttctgc ctcggaggta
    21841 tttaaacaat gaataaaatc ctgttagttg atgatgaccg agagctgact tccctattaa
    21901 aggagctgct cgagatggaa ggcttcaacg tgattgttgc ccacgatggg gaacaggcgc
    21961 ttgatcttct ggacgacagc attgatttac ttttgcttga cgtaatgatg ccgaagaaaa
    22021 atggtatcga cacattaaaa gcacttcgcc agacacacca gacgcctgtc attatgttga
    22081 cggcgcgcgg cagtgaactt gatcgcgttc tcggccttga gctgggcgca gatgactatc
    22141 tcccgaaacc gtttaatgat cgtgagctgg tggcacgtat tcgcgcgatc ctgcgccgtt
    22201 cgcactggag cgagcaacag caaaacaacg acaacggttc accgacactg gaagttgatg
    22261 ccttagtgct gaatccaggc cgtcaggaag ccagcttcga cgggcaaacg ctggagttaa
    22321 ccggtactga gtttaccctg ctctatttgc tggcacagca tctgggtcag gtggtttccc
    22381 gtgaacattt aagccaggaa gtgttgggca aacgcctgac gcctttcgac cgcgctattg
    22441 atatgcacat ttccaacctg cgtcgtaaac tgccggatcg taaagatggt cacccgtggt
    22501 ttaaaacctt gcgtggtcgc ggctatctga tggtttctgc ttcatgatag gcagcttaac
    22561 cgcgcgcatc ttcgccatct tctggctgac gctggcgctg gtgttgatgt tggttttgat
    22621 gttacccaag ctcgattcac gccagatgac cgagcttctg gatagcgaac agcgtcaggg
    22681 gctgatgatt gagcagcatg tcgaagcgga gctggcgaac gatccgccca acgatttaat
    22741 gtggtggcgg cgtctgttcc gggcgattga taagtgggca ccgccaggac agcgtttgtt
    22801 attggtgacc accgaaggcc gcgtgatcgg cgctgaacgc agcgaaatgc agatcattcg
    22861 taactttatt ggtcaggccg ataacgccga tcatccgcag aagaaaaagt atggccgcgt
    22921 ggaactggtc ggtccgttct ccgtgcgtga tggcgaagat aattaccaac tttatctgat
    22981 tcgtccggcc agcagttctc aatccgattt cattaactta ctgtttgacc gcccgctatt
    23041 actgctgatt gtcaccatgt tggtcagtac gccgctgctg ttgtggttgg cctggagtct
    23101 ggcaaaaccg gcgcgtaagc tgaaaaacgc tgccgatgaa gttgcccagg gaaacttacg
    23161 ccagcacccg gaactggaag cggggccaca ggaattcctt gccgcaggtg ccagttttaa
    23221 ccagatggtc accgcgctgg agcgcatgat gacctctcag cagcgtctgc tttctgatat
    23281 ctctcacgag ctgcgcaccc cgctgacgcg tctgcaactg ggtacggcat tactgcgccg
    23341 tcgtagcggt gaaagcaagg aactggagcg tattgaaacc gaagcgcaac gtctggacag
    23401 catgatcaac gatctgttgg tgatgtcacg taatcagcaa aaaaacgcgc tggttagcga
    23461 aaccatcaaa gccaaccagt tgtggagtga agtgctggat aacgcggcgt tcgaagccga
    23521 gcaaatgggc aagtcgttga cagttaactt cccgcctggg ccgtggccgc tgtacggcaa
    23581 tccgaacgcc ctggaaagtg cgctggaaaa cattgttcgt aatgctctgc gttattccca
    23641 tacgaagatt gaagtgggct ttgcggtaga taaagacggt atcaccatta cggtggacga
    23701 cgatggtcct ggcgttagcc cggaagatcg cgaacagatt ttccgtccgt tctatcggac
    23761 cgatgaagca cgcgatcgtg aatctggcgg tacaggattg gggctggcga ttgttgaaac
    23821 cgccattcag cagcatcgtg gctgggtgaa ggcagaagac agcccgctgg gcggtttacg
    23881 gctggtgatt tggttgccgc tgtataagcg gagttaaact ccgcatttgt aggcaggata
    23941 aggcgtttac gccgcatccg gcatttgagc aggatgcctg atgcgacgct gatagcgtct
    24001 tatcaggcct acactccggg ggttattttc cccacaaccg gcgggaaaaa tcttcgatct
    24061 tgccgctcag tcggcgcttt tgcatcgtcc gcgtccagct tttcgataac ccggcggcgg
    24121 agagtaaacg gtgatactga tcgtcatcaa acggcatatg ccatgcgatg gcggcagcct
    24181 cctgcacggt cacatcgctg acacgggaaa ccaactccag cggcgcgtcc gcagacactt
    24241 tccctggcgc tatcacgcta tacaaccacc ccaccttacc tgtgttttgc atcaactgcg
    24301 ctatatcgct gatatcaaaa tggtaattga gtttatagca aggcgagcgc ggctggctga
    24361 cctgaattaa tgcctctccc cagcggaaaa tatcgcccat ataaacatta ctttccgtca
    24421 ggccgtcggt tgagaggttt tcaccaaacg caggcgcaac aaacaactcc gcctgttccg
    24481 gaaattcccg cgcccagtag agataatgct cacgaggata atgacacagc gctctgtctg
    24541 gcccaccatg aacctttttc tccgcctgct cgtcaccttc cagccccagc tccgtcagca
    24601 tcaactcacc atcaacctgg attttggcaa ttgcgctggg tttgccttcg ggataagcct
    24661 gaatcttgcc tgtgtataca tcaaccggat atcgcatctg ttccctctcc acgagaaatt
    24721 tccccattag accatattta ttttatgtca ggcgtgctgt tttcgcggta agtatccagg
    24781 caggaactca atagcaaacc ggacgaacgt tagttcatcc ggctgtgggg attaaggaag
    24841 caggtttatt tcaccgtacc taaaatttcg attttcgctg ctctggcttt gactttacgt
    24901 gaccagatag aggtaagaat tggcaccaga atcgaagtca caatgaccgc cgtcgctacc
    24961 agcgaagttg ctgccggagc catcggttta aacgcaggca ccatttctgc aatcagcata
    25021 ggtgtcgcta ccgcggcccc tgcggaactg gaagcggcaa ttccggcagt gccatcgcca
    25081 ccgccaatca atttatcggc gataatcaac ggaataccgg tcacgataat tactgccaca
    25141 cccaacagga tccccagcaa accagtctgg gcaatcacag tcaaatcaat ggtattgccc
    25201 agcgcgaagg caaagaatgg aatcagcgtt tgcaccgctt tgctgaaaaa ttctcgtaat
    25261 tcagggtcaa ggttccccag ggcaaagccc accaggaacg gtaatactgc gccgacgaaa
    25321 acatgcggtt caaacgaggc aatccctgca gtgcccagaa taatcatcgt catgagcggc
    25381 ccggactcca acgacatcaa cacaaatgcc ccagcttctt cttttgtgcc gtactgctgc
    25441 atgatggaag cgtaaagtcc gccattggtc atatccatcg ccgccaccag cgccagcgtt
    25501 gaaagtccgg caaagaatcc aacttcaaca ccatgttccg gaataatgcg cgaggcaatt
    25561 gccgcaacca cccacgcgac ggcaattttt gttaccacca gcgtaccgga tttacgcaat
    25621 accgttcccg tcgcgcttaa ttttattgac gcccccatgc aaaaaaacca caccgccaga
    25681 atggacaccg taccggtaat catcccgttg gtgaatgatc caaaatattt ccccgccccc
    25741 ggcgagaagg tgtggcacag tgcgccaagg aatagcggga cgagcatcat ccccccccgg
    25801 gattttctca atcgagcgtt ttatctgcat ttccatcacc tgtcggcata tttttaaggg
    25861 ctgatgcgta attcctcaat ggagcatcag tgttgatgga aacgaagata gacattcatt
    25921 taatcaataa aagtgatcaa aaccacatca attgaaacgc tgttttattt ttatcggatc
    25981 attgttttta ttttgaaatt gatcacaaaa aaaccaccgc cgttggcgat ggttcatttt
    26041 ttaagctgat atgcggcgta aacgcctcat tgcagcaggc ggcaaatgat tattttttcg
    26101 ccgcgaaacg tgccgctgct tcatcccagt tcaccacgtt ccagaactct ttgatgtagt
    26161 ccggacggcg gttctggaat ttcaggtagt aagcatgttc ccacacatcc aggcccagaa
    26221 tcgggaagcc ggaagcgcca gaaatagctt cacccatcag cggggaatcc tggttagcag
    26281 tagaaaccac cgccagttta tcgcctttca gcaccagcca tgcccagccg gaaccaaagc
    26341 gggaagctgc cgctttctca aattctgctt tgaagttatc aacggagccg aagtcacgtt
    26401 cgatagccgc tttcaggtca ccctgtaagg tggtgccttt tttcagacct ttccagaaca
    26461 ggctgtggtt agcgtgaccg ccagcgttgt tgcgcagtac ggttttcttg tctgctggca
    26521 gctggtccag tttggtgatc agctcttcaa ccggcaggtt ggcaaattct ggcaggcttt
    26581 ccagcgccgc gttggcgttg ttgacgtagg tctgatggtg tttggtgtgg tggatttcca
    26641 tggtctgctt atcgaagtgc ggttccaggg catcgtaagc atacggcagg gatggcaggg
    26701 tatagctcat attcatctcc agtattgtcg ggcggccgat tgttaatgcg cgtaagcagt
    26761 tggttcatta tagttaatta aatgatattg aaaatgatta tcaatgccgt acttttcgta
    26821 agggtatggt tttgcaggaa aattcccgag atgtgaagca aatcacccac ttaatgccgt
    26881 gattgccagt aaatcgacaa cggcggcaac aggcgaaagg ttaatcgaca gcacgatttt
    26941 tacactcatc tcgtcggaga tgtgacgcga cgaaaaatga tgaggataag aagatgagta
    27001 acgcgattac gatggggata ttttggcatt tgatcggcgc ggccagtgca gcctgttttt
    27061 acgctccgtt caaaaaagta aaaaaatggt catgggaaac catgtggtca gttggtggga
    27121 ttgtttcgtg gattattctg ccgtgggcca tcagcgccct gttactgccg aatttctggg
    27181 cgtattacag ctcgtttagt ctctctacgc tgctgcctgt ttttctgttc ggcgctatgt
    27241 gggggatcgg taatatcaac tacggcctga ccatgcgtta tctcggcatg tcgatgggaa
    27301 ttggcatcgc cattggcatt acgttgattg tcggtacgct gatgacgcca attatcaacg
    27361 gcaatttcga tgttctgctt aataccgaag gcggacgcat gacgttgctc ggcgttctgg
    27421 tggcgctgat tggtgtaggg attgtgactc gcgccgggca gttgaaagag cgcaaaatgg
    27481 gcattaaagc cgaagagttc aacctgaaaa aagggctggt gctggcggtg atgtgcggca
    27541 ttttctctgc cggtatgtct tttgcgatga acgccgcaaa accgatgcat gaagccgctg
    27601 ccgcactggg cgtcgatcca ctgtatgtcg ctctgccaag ctatgtcatc atcatgggcg
    27661 gcggcgcgat catcaacctc ggtttctgct tcattcgtct ggcaaaagtg aaggatttgt
    27721 cgctaaaagc cgacttctcg ctggcaaaac cgctgatcat tcacaatgtg ttactctcag
    27781 cactgggcgg gttgatgtgg tatctgcaat tctttttcta tgcctggggc cacgcccgca
    27841 ttccggcgca gtatgactac atcagttgga tgctgcatat gagtttctat gtattgtgcg
    27901 gcggtatcgt cgggctggtg ctgaaagagt ggaacaatgc aggtcgccgt ccggtaacgg
    27961 tgttgagcct cggttgtgtg gtgattattg tcgccgccaa catcgtcggc atcggcatgg
    28021 cgaattaatc tttctgcgaa ttgagatgac gccactggct gggcgtcatc ccggtttccc
    28081 gggtaaacac caccgaaaaa tagttactat cttcaaagcc acattcggtc gaaatatcac
    28141 tgattaacag gcggctatgc tggagaagat attgcgcatg acacactctg acctgccgca
    28201 gatattgatt gatggtcatt ccagtctgct ggcgaaattg ctgacgcaaa acgcgctcac
    28261 tgcacgatgc ctcatcacaa aatttatcca gcgcaaaggg acttttcagg ctagccgcca
    28321 gccgggtaat cagcttatcc agcaacgttt cgctggatgt tggcggcaac gaatcactgg
    28381 tgtaacgatg gcgattcagc aacatcacca actgcccgaa cagcaactca gccatttcgt
    28441 tagcaaacga cacatgctga ctactttcat gctcaagctg accgataacc tgccgcgcct
    28501 gcgccatccc cacgctacct aagcgccagt gtggttgccc tgcgctggcg ctaaatcccg
    28561 gaatcgctcc ctgccagtca agattcagct tcagccgctc cgggcaataa ataatattct
    28621 gcaaaaccag atcgttaacg gaagcgtagg agtgtttatc gtcagcatga atgtaaaaga
    28681 gatcgccacg ggtaatgcga taagggcgat cgttgagtac atgcagacca ttaccgcgcc
    28741 agacaatcac cagctcacaa aaatcatgtg tatgttcggc aaaaacatct tgcggataac
    28801 ggtcagccac agcgactgcc tgctggtcgc tggcaaaaaa atcatctttg agaagtttta
    28861 actgatgcgc caccgtggct acctcggcca gagaacgaag ttgattattc gcaatatggc
    28921 gtacaaatac gttgagaaga ttcgcgttat tgcagaaagc catcccgtcc ctggcgaata
    28981 tcacgcggtg accagttaaa ctctcggcga aaaagcgtcg aaaagtggtt actgtcgctg
    29041 aatccgcagc gataggcgat gtcagtaacg ctggcctcgc tgtgacgtag cagatgtcgg
    29101 gccttcatca gtcgcaggcg gttcaggtaa cgctgaggcg tcagtcccgt ttgctgctta
    29161 agctgccgat gtagcgtacg cagtgaaaga gaaaattgat ccgccacggc atcccaattc
    29221 acctcatcgg caaaatggtc ctccagccag gccagaagca agttgagacg tgatgcgctg
    29281 ttttccaggt tctcctgcaa actgctttta cgcagcaaga gcagtaattg cataaacaag
    29341 atctcgcgac tggcggtcga gggtaaatca ttttcccctt cctgctgttc catctgtgca
    29401 accagctgtc gcacctgctg caatacgctg tggttaacgc gccagtgaga cggatactgc
    29461 ccatccagtt cttgtggcag caactgattc agcccggcga gaaactgaaa tcgatccggc
    29521 gagcgataca gcacattggt cagacacaga ttatcggtat gttcatacag atgccgatca
    29581 tgatcgcgta cgaaacagac cgtgccaccg gtgatggtat agggctgccc attaaacaca
    29641 tgaatacccg tgccatgttc gacaatcaca atttcatgaa aatcatgatg atgttcagga
    29701 aaatccgcct gcgggagccg gggttctatc gccacggacg cgttaccaga cggaaaaaaa
    29761 tccacactat gtaatacggt catactggcc tcctgatgtc gtcaacacgg cgaaatagta
    29821 atcacgaggt caggttctta ccttaaattt tcgacggaaa accacgtaaa aaacgtcgat
    29881 ttttcaagat acagcgtgaa ttttcaggaa atgcggtgag catcacatca ccacaatgaa
    29941 gcaaattgtg aacatcatca cgttcatctt tccctggttg ccaatggccc attttcctgt
    30001 cagtaacgag aaggtcacga attcaggcgc tttttagact ggtcgtaatg aaattcagca
    30061 ggatcacatt atgacctttc gcaattgtgt cgccgtcgat ctcggcgcat ccagtgggcg
    30121 cgtgatgctg gcgcgttacg agcgtgaatg ccgcagcctg acgctgcgcg aaatccatcg
    30181 ttttaacaat ggcctgcata gccagaacgg ttatgtcacc tggaatgtgg atagcctgga
    30241 aagtgccatt cgccttggat taaacaaggt gtgcgaggaa gggattcgta tcgatagcat
    30301 tgggattgat acctggggcg tggactttgt gctgctcgac caacagggtc agcgtgtggg
    30361 cctgcccgtt gcttatcgcg atagccgctc caatggccta atggcgcagg cacagcaaca
    30421 actcggcaaa cgcgatattt atcaacgtag cggcatccag tttctgccct tcaatacgct
    30481 ttatcaactg cgtgcgctga cggagcaaca acctgaactt attccacaca ttgctcacgc
    30541 tctgctgatg ccggattact tcagctatcg cctgaccggc aagatgaact gggaatatac
    30601 caatgccacc accacacaac tggtcaatat caatagcgac gactgggacg agtcgctact
    30661 ggcgtggagc ggggccaaca aagcctggtt tggtcgcccg acgcatccgg gtaatgtcat
    30721 tgggcactgg atttgcccgc agggtaatga gattccggtg gtcgccgttg ccagccatga
    30781 taccgccagc gcggttatcg cctcgccgtt aaacggttca cgcgctgctt atctctcttc
    30841 tggcacctgg tcattgatgg gcttcgaaag ccagacgcca tttaccaatg acacggcgct
    30901 ggcagccaac ataaccaatg aaggcggggc ggaaggtcgc tatcgggtgc tgaaaaatat
    30961 tatgggctta tggctgcttc agcgagtgct tcaggagcgg caaatcaacg atctcccggc
    31021 gcttatcgcc gcgacacagg cacttccggc ctgccgcttc atcatcaatc ccaatgacga
    31081 tcgctttatt aatcctgagg cgatgtgcag cgaaattcag gctgcgtgtc gggaaacggc
    31141 gcaaccgatc ccggaaagtg atgctgaact ggcgcagcta cgcggtgaag atttctcgca
    31201 actgcatatt gtcggcggcg gctgccagaa cacgctgctc aaccagctat gtgccgatgc
    31261 ctgcggtatt cgggtgatcg ccgggcctgt tgaagcctcg acgctcggca atatcggcat
    31321 ccagttaatg acgctggatg aactcaacaa tgtggatgat ttccgtcagg tcgtcagcac
    31381 caccgcgaat ctgaccacct ttacccctaa tcctgacagt gaaattgccc actatgtggc
    31441 gcagcttcac tctacacgac agacaaagga gctttgcgca tgaccactca actggaacag
    31501 gcctgggagc tggcgaaaca gcgtttcgcg gcggtgggga ttgatgtcga ggaggcgctg
    31561 cgccaacttg atcgtttacc cgtttcaatg cactgctggc agggcgatga tgtttccggt
    31621 tttgaaaacc cggaaggttc gctgaccggg gggattcagg ccacaggcaa ttatccgggc
    31681 aaagcgcgta atgccagtga gctacgtgcc gatctggaac aggctatgcg gctgattccg
    31741 gggccgaaac ggcttaattt ccatgccatc tatctggaat cagatacgcc agtctcgcgc
    31801 gaccagatca aaccagagca cttcaaaaac tggggtgaat gggcgaaagc caatcagctc
    31861 ggtctggatt ttaacccctc ctgcttttcg catccgctaa gcgccgatgg ctttacgctc
    31921 tcgcatcccg acgacagcat tcgccagttc tggattgatc actgcaaagc cagccgtcgc
    31981 gtttcggcct attttggcga gcaactcggc acaccatcgg tgatgaacat ctggatcccg
    32041 gatggtatga aagatatcac cgttgaccgt ctcgccccgc gtcagcgtct gctggcagca
    32101 ctggatgagg tgatcagcaa gaagctaaac cctgcgcacc atatcgacgc cgttgagagc
    32161 aaattgtttg gcattggcgc agagagctac acggttggct ccaatgagtt ttacatgggg
    32221 tatgccacca gccgccagac tgcgctgtgc ctagacgccg ggcacttcca cccgactgaa
    32281 gtgatttccg acaagatttc cgccgccatg ctgtatgtgc cgcagttgct gctgcacgtc
    32341 agccgtccgg ttcgctggga ctgcgatcac gtagtgctgc tggatgatga aacccaggca
    32401 attgccagtg agattgtgcg tcacgatctg tttgaccggg tgcatatcgg ccttgacttc
    32461 ttcgatgcct ctatcaaccg cattgccgcg tgggtcattg gtacacgcaa tatgaaaaaa
    32521 gccctgctgc gtgcgttgct ggaacctacc gctgagctgc gcaagctgga agcggcgggc
    32581 gattacactg cgcgtctggc actgctggaa gagcagaaat cgttgccgtg gcaggcggtc
    32641 tgggaaatgt attgccaacg tcacgatacg ccagcaggta gcgaatggct ggagagcgtg
    32701 cgggcttatg aaaaagaaat tttgagccag cgtgggtaaa cactgccgga tgcggcgcga
    32761 gcgccttatc cggcctacgg gtcgtgcatc tgacaacacc gaatttacag gaacacagaa
    32821 catgcaaaac attacccagt cctggtttgt ccagggaatg atcaaagcca ccaccgatgc
    32881 ctggctgaaa ggctgggacg aacgcaacgg cggcaacctg acgctgcgcc tggatgacgc
    32941 cgatatcgcg ccttataaag acaacgtcca tgcacaaccg cgctatatcc cgctcagcca
    33001 gcccatgcct ttactggcaa atacaccgtt tattgtcacc ggctccggca aattcttccg
    33061 taacgtccag ctcgatcctg cagctaactt aggcgtcgta aaagtcgaca gcgatggcgc
    33121 gggctaccat attctctggg ggttaaccaa cgaagccgtc ccgacatccg aactcccggc
    33181 gcactttctc tcccactgcg agcgcattaa agccaccaac ggtaaagatc gggtgatcat
    33241 gcactgccac gccaccaacc tgatcgccct cacctatgta ctggaaaacg acaccgcggt
    33301 ctttactcgc caactgtggg aaggcagcac cgagtgtctg gtggtattcc cggatggcgt
    33361 tggcattctg ccgtggatgg tgcccggcac ggacgaaatc ggccaggcga ccgcacaaga
    33421 gatgcaaaaa cattcgctgg tgttgtggcc cttccacggc gtcttcggca gcgggtcgac
    33481 gttggatgaa acattcggtt taatcgacac cgcagaaaaa tcagcacaag tattagtgaa
    33541 ggtttattcg atgggcggca tgaaacagac catcagccgt gaagagttga tagcgctcgg
    33601 caagcgcttc ggcgttacac cactagccag tgcgctggcg ctgtaaggag caaacatgat
    33661 ccgcaaagcc tttgtcatgc aggttaaccc cgacgcccac gaagagtatc agcgtcggca
    33721 taaccccatc tggccggaac tggaagcagt gttgaaatct cacggtgcgc ataactacgc
    33781 catttacctc gacaaagcgc gtaatctgct gtttgccacg gtagagattg aatctgaaga
    33841 acggtggaat gcggtagcca gtactgatgt ttgccaacgt tggtggaaat atatgaccga
    33901 tgttatgccc gctaacccgg ataacagccc ggtgagtagc gagctg
//
LOCUS       DAJVUJ010000026        33031 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.026, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000026 DAJVUJ010000000
VERSION     DAJVUJ010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33031
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.026"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..258
                     /locus_tag="OQQ48_001160"
     CDS             <1..258
                     /locus_tag="OQQ48_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HCS1952006.1"
                     /translation="GLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNGLSV
                     VMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            268..1642
                     /gene="gspE"
                     /locus_tag="OQQ48_001161"
                     /pseudo
     CDS             268..1642
                     /gene="gspE"
                     /locus_tag="OQQ48_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414649.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
     gene            1632..2834
                     /gene="hofC"
                     /locus_tag="OQQ48_001162"
     CDS             1632..2834
                     /gene="hofC"
                     /locus_tag="OQQ48_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HCS1952007.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRALLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAGQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWSW
                     LLVLFGFLLAIANKLLMRRPTWLIVRQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNTGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(2869..3912)
                     /gene="guaC"
                     /locus_tag="OQQ48_001163"
     CDS             complement(2869..3912)
                     /gene="guaC"
                     /locus_tag="OQQ48_001163"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCS1952008.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSDQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(4068..4112)
                     /locus_tag="OQQ48_001164"
     CDS             complement(4068..4112)
                     /locus_tag="OQQ48_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1952009.1"
                     /translation="MIIKTLKMSARKRN"
     gene            4137..4757
                     /gene="coaE"
                     /locus_tag="OQQ48_001165"
     CDS             4137..4757
                     /gene="coaE"
                     /locus_tag="OQQ48_001165"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCS1952010.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            4757..5500
                     /gene="zapD"
                     /locus_tag="OQQ48_001166"
     CDS             4757..5500
                     /gene="zapD"
                     /locus_tag="OQQ48_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HCS1952011.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDSENGQVPERLDFELACC"
     gene            5510..5707
                     /gene="yacG"
                     /locus_tag="OQQ48_001167"
     CDS             5510..5707
                     /gene="yacG"
                     /locus_tag="OQQ48_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HCS1952012.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(5814..6203)
                     /gene="mutT"
                     /locus_tag="OQQ48_001168"
     CDS             complement(5814..6203)
                     /gene="mutT"
                     /locus_tag="OQQ48_001168"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HCS1952013.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLAYEFPDRHITLWFWLVESWEGVPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(6263..8968)
                     /gene="secA"
                     /locus_tag="OQQ48_001169"
     CDS             complement(6263..8968)
                     /gene="secA"
                     /locus_tag="OQQ48_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCS1952014.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(9030..9542)
                     /gene="secM"
                     /locus_tag="OQQ48_001170"
     CDS             complement(9030..9542)
                     /gene="secM"
                     /locus_tag="OQQ48_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HCS1952015.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNVPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQYAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(9773..10690)
                     /gene="lpxC"
                     /locus_tag="OQQ48_001171"
     CDS             complement(9773..10690)
                     /gene="lpxC"
                     /locus_tag="OQQ48_001171"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HCS1952016.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFIAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLTFKAPSAVLA"
     gene            complement(10791..11942)
                     /gene="ftsZ"
                     /locus_tag="OQQ48_001172"
     CDS             complement(10791..11942)
                     /gene="ftsZ"
                     /locus_tag="OQQ48_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCS1952017.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(12003..13265)
                     /gene="ftsA"
                     /locus_tag="OQQ48_001173"
     CDS             complement(12003..13265)
                     /gene="ftsA"
                     /locus_tag="OQQ48_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCS1952018.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(13262..14092)
                     /gene="ftsQ"
                     /locus_tag="OQQ48_001174"
     CDS             complement(13262..14092)
                     /gene="ftsQ"
                     /locus_tag="OQQ48_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HCS1952019.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKETAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQLAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(14094..15014)
                     /gene="ddlB"
                     /locus_tag="OQQ48_001175"
     CDS             complement(14094..15014)
                     /gene="ddlB"
                     /locus_tag="OQQ48_001175"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCS1952020.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLDASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(15007..16482)
                     /gene="murC"
                     /locus_tag="OQQ48_001176"
     CDS             complement(15007..16482)
                     /gene="murC"
                     /locus_tag="OQQ48_001176"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCS1952021.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFLGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(16536..17603)
                     /gene="murG"
                     /locus_tag="OQQ48_001177"
     CDS             complement(16536..17603)
                     /gene="murG"
                     /locus_tag="OQQ48_001177"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCS1952022.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(17600..18844)
                     /gene="ftsW"
                     /locus_tag="OQQ48_001178"
     CDS             complement(17600..18844)
                     /gene="ftsW"
                     /locus_tag="OQQ48_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HCS1952023.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVVFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(18844..20160)
                     /gene="murD"
                     /locus_tag="OQQ48_001179"
     CDS             complement(18844..20160)
                     /gene="murD"
                     /locus_tag="OQQ48_001179"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCS1952024.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDAECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     QVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(20163..21245)
                     /gene="mraY"
                     /locus_tag="OQQ48_001180"
     CDS             complement(20163..21245)
                     /gene="mraY"
                     /locus_tag="OQQ48_001180"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCS1952025.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(21239..22597)
                     /gene="murF"
                     /locus_tag="OQQ48_001181"
     CDS             complement(21239..22597)
                     /gene="murF"
                     /locus_tag="OQQ48_001181"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCS1952026.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(22594..24081)
                     /gene="murE"
                     /locus_tag="OQQ48_001182"
     CDS             complement(22594..24081)
                     /gene="murE"
                     /locus_tag="OQQ48_001182"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HCS1952027.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVEQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKV
                     IDVNYHDSGVTIRFSSSWGDGEIESHLIGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTVPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(24068..25834)
                     /gene="ftsI"
                     /locus_tag="OQQ48_001183"
     CDS             complement(24068..25834)
                     /gene="ftsI"
                     /locus_tag="OQQ48_001183"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HCS1952028.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(25850..26215)
                     /gene="ftsL"
                     /locus_tag="OQQ48_001184"
     CDS             complement(25850..26215)
                     /gene="ftsL"
                     /locus_tag="OQQ48_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCS1952029.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(26212..27153)
                     /gene="rsmH"
                     /locus_tag="OQQ48_001185"
     CDS             complement(26212..27153)
                     /gene="rsmH"
                     /locus_tag="OQQ48_001185"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCS1952030.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(27155..27613)
                     /gene="mraZ"
                     /locus_tag="OQQ48_001186"
     CDS             complement(27155..27613)
                     /gene="mraZ"
                     /locus_tag="OQQ48_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCS1952031.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(28215..29219)
                     /gene="cra"
                     /locus_tag="OQQ48_001187"
     CDS             complement(28215..29219)
                     /gene="cra"
                     /locus_tag="OQQ48_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HCS1952032.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANAPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHLMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(29301..29387)
                     /locus_tag="OQQ48_001188"
     CDS             complement(29301..29387)
                     /locus_tag="OQQ48_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HCS1952033.1"
                     /translation="MCNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(29399..29890)
                     /gene="ilvN"
                     /locus_tag="OQQ48_001189"
     CDS             complement(29399..29890)
                     /gene="ilvN"
                     /locus_tag="OQQ48_001189"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCS1952034.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIREVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(29893..31617)
                     /gene="ilvI"
                     /locus_tag="OQQ48_001190"
     CDS             complement(29893..31617)
                     /gene="ilvI"
                     /locus_tag="OQQ48_001190"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HCS1952035.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVVAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            31736..31780
                     /locus_tag="OQQ48_001191"
     CDS             31736..31780
                     /locus_tag="OQQ48_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1952036.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(31935..32879)
                     /gene="leuO"
                     /locus_tag="OQQ48_001192"
     CDS             complement(31935..32879)
                     /gene="leuO"
                     /locus_tag="OQQ48_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HCS1952037.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT         7930 a         9271 c         8034 g         7796 t
ORIGIN      
        1 ggattagata cctgcgacgg tggcagcaat ggcattccct cgcctaccac cacccgctat
       61 gtttcagcca tgagtgtggc aaaaggcgtg gtgtcgctga ccgggcaaga aagtctcaat
      121 ggactaagcg tcgtcatgac gccaggttgg gataacgcaa acggcgtcac cggctgggcg
      181 cgcaactgca atattcaaag tgacagcgca ttgcagcaag cctgcgaaga tgtcttccgc
      241 tttgatgacg ccaactaagg agcggcaatg aatattccac agctcaccgc cctgtgtctg
      301 cgttatcagg gagtcttgct ggatgccagc gaagaggtgg ttcatgttgc ggtagtcgat
      361 gcaccttcgc atgagttgct ggacgcattg catttcgcta ccaccaaacg tattgagatc
      421 acctgctgga cgcgccaaca aatggaaggc cacgccagtc gcacacaaca gacattgccc
      481 gtagctgttc aggagaagca tcagtcgaaa gcagagttgc tgactcgaac gttacaatct
      541 gcgctggaac aacgtgcgtc tgatattcat atcgaaccag cggacaatgc ctaccgcatc
      601 cgcttgcgta tcgacggcgt attgcatcct ttaccggatg tttcaccgga tgccggagtc
      661 gcattaaccg ccagattaaa agtgctggga aacctggata ttgcggaaca tcgcctgccg
      721 caggacgggc aattcactgt cgaactggca ggaaacgccg tctcatttcg tattgcgacc
      781 ttaccatgtc ggggtggtga aaaggtggta ttaaggttgt tacagcaggt gaaccaggca
      841 ctggatgtta acacgctggg aatgcagccg ttacaactgg cggactttgc tcatgccttg
      901 caacaaccac agggactggt gctggtaact ggccctacag gcagcggcaa aacggtcacg
      961 ctttatagtg ccctgcaaaa gctgaatacc gctgacatta atatttgtag cgtcgaagat
     1021 ccggttgaga tccccatagc cggactaaac cagacgcaaa tccatccgcg tgccgggctc
     1081 acctttcagg gcgttttgcg tgcgttattg cgccaggatc ctgacgtcat catgatccga
     1141 tggcgaaacg gcagaaattg ccattaaagc cgcgcaaacc ggtcacctgg tgttgtctac
     1201 cctacacact aattccacct gcgaaacgct ggtacgttta cagcaaatgg gagtcgcccg
     1261 ctggatgctc tcatcagcgc ttacgctggt aatagcccag cgtctggtac gtaaattttg
     1321 cccacattgt cgccagcagc aaggggagcc catccatatt ccagacaatg tatggccgtc
     1381 gccgctgccc cactggcagg cacccggttg tgtacattgc taccacggtt tttatggtcg
     1441 taccgccttg tttgaagtcc tgcccattac accagccata cgccagctta tttccgctaa
     1501 taccgacgtt gaatcgctgg aaacgcacgc acgacaggcg ggtatgcgta cgctttttga
     1561 aaacggctgc ctagccgtgg agcaaggctt aaccaccttt gaagagttaa tccgcgtact
     1621 ggggatgccg catggcgagt aagcaactct ggcgctggca tggcataacc ggcgacggca
     1681 atgcgcaaga tgggatgcta tgggcagaga gccgtgcttt gctgctcatg gcactacagc
     1741 aacagatggt taccccactt agcctgaagc gaatcgccat caattctgcg cagtggcgag
     1801 gagataaaag cgcggaagtc attcatcaac tggcgacgct actcaaagcc gggttaacgc
     1861 tttctgaagg gctggcactg ctggcgggac agcatcccag taagcaatgg caagcgttgc
     1921 tgcaatcgct ggcgcacgat ctcgaacagg gcattgcttt ttccaatgcc ttattaccct
     1981 ggtcagaggt atttccgcca ctctatcagg cgatgatccg cacgggtgaa ctgaccggta
     2041 agctggatga atgctgcttt gaactggcgc gtcagcaaaa agcccagcgt cagttgaccg
     2101 acaaagtgaa atcagcgtta cgttatccca tcatcatttt agcgatggca atcatggtgg
     2161 ttgtggcaat gctgcatttt gttctgccgg agtttgccgc tatctataag accttcaaca
     2221 ccccactacc ggcactaacg caggggatca tgacgctggc agactttagt ggcgaatgga
     2281 gctggctgct ggtgttgttc ggctttctgc tggcgatagc caataagttg ctgatgcgcc
     2341 gaccgacctg gcttatagtg cggcagaaat tgctgttacg catcccgatt atgggttcac
     2401 tgatgcgggg acaaaaactc acgcagatct ttacgattct ggcgctgaca caaagtgcag
     2461 gcattacttt tttgcagggc gtagagagcg tcagagaaac aatgcgctgc ccgtactggg
     2521 tgcaacttct gacacaaatc cagcacgata tcagtaacgg tcatcccatc tggctggcgc
     2581 taaaaaatac cggggagttt agcccgctct gtttgcaatt agtgagaaca ggagaggcat
     2641 ccggctcgct ggacctcatg ttagacaacc tcgcccatca tcatcgggat aacacaatgg
     2701 cgctggcgga taacctcgca gccttactgg aaccggcgtt gctgatcata acgggaggaa
     2761 ttatcggtac gctggtggtg gcgatgtatc tgccaatttt ccatttaggc gatgcgatga
     2821 gtgggatggg ataacgctgg cgtgttgctc cacaccagcg ttggaagatt acaggttgtt
     2881 gaagatgcgg ttttcttgtt cctgcacacg gataaatgtg gtgcgcttgg tcagttcttt
     2941 caggcgtgaa gccccaacgt atgtacaagc tgaacgcagg ccgcccaaaa tatcacgcgc
     3001 ggtattttca accgggcctc gcagcggcag cttaacggtt ttaccttctg ctgcgcgata
     3061 ttccgcaacg ccgccaacgt gacgtttcat cgcagactcg gagctcatgc cgtagaacag
     3121 cataaatttc tcgccgttct cctcaacgat gcgaccgccg ctctcttcgt ggcccgccag
     3181 catgccgcca agcatgacga aatcggcacc gccgccaaag gcttttgcga catcgcccgg
     3241 cgtggtgcag ccaccgtcgc tgacgatcat tccgcccaga ccgtgcgccg catcggcaca
     3301 ttcgattacc gcagaaagct gcggataacc gacgcctgtt ttgacgcgag ttgtacaaac
     3361 agaacctggg ccaatgccaa ctttaacgat atcagcaccg gagaggataa gctcctcaca
     3421 catttcacca gtcactacgt tgccagcaca aatggttttg gtcggccacg cttcacgcgc
     3481 tttcgcaacg aactgcacga agtgttcgga ataaccattc gccacatcaa tacaaacaaa
     3541 gtttaatgcc gggttcaggt cgagaatctg tttagttttt tcgaaatccg catcagacgt
     3601 accggtagaa accatcacat gtttcagcac atcagcggaa gaattgttga taaacgcttg
     3661 ccactcttcg acagaatagt gtttatgcac agcagtcaaa atatcaaaag aagccagcgc
     3721 agaggccata gaaaatgtac ctacggtgtc catatttgcg gcgataatcg gcacgccgga
     3781 ccagctctga tctgaatgtt tgaaggtgaa ttgacgttcc agttcaacat cggaacggct
     3841 tttaagagtg gagcgtttag ggcggatgag aacgtcttta aaacctaact tcagatcttc
     3901 ttcaatacgc atgtgcggat tcctggggtt aatggctata agggtaagag cgcaactcca
     3961 gtgacgctat catacgcact aatcaatgcg gcgcaagact gcgaaattgt ctttttttac
     4021 gctacaatcc cataaattta cctggtaaat agtgagcgaa cgtttcatca atttcgcttt
     4081 cgtgcactca tttttaacgt tttgataatc atgatgaatt cccgggaata taaattatga
     4141 ggtatatagt tgccttaacg ggaggcattg gcagtggcaa gagtaccgtt gccaatgcgt
     4201 ttgctgatct cggaattaac gtcattgatg ccgatattat tgcgcgtcag gtggttgaac
     4261 caggtgcacc tgcgctacat gccattgctg atcactttgg cgctaacatg attgctgctg
     4321 atggaacatt gcagcgccgg gccttgcgcg agcggatctt cgccaacccg gaagagaaaa
     4381 actggcttaa cgccctgctg catccgctga ttcagcaaga gacgcaacac cagatccagc
     4441 aagctacttc cccctatgta ctgtgggttg tgccattgct ggtagaaaac tcactgtata
     4501 aaaaagcgaa tcgagtgctg gtggtggatg tcagcccaga aacgcaactt aagcgcacca
     4561 tgcagcgcga tgatgtaact cgcgagcatg tcgaacaaat ccttgctgct caggcaacgc
     4621 gcgaagcccg ccttgccgtg gcagatgacg tcattgataa taacggcgca ccggatgcta
     4681 tcgcatcgga tgttgcccgc ctgcacgcac actatttgca gcttgcgtcg cagtttgtct
     4741 cacaggaaaa accgtaatgc agacccaggt cctttttgaa catccactaa atgaaaaaat
     4801 gcgtacatgg ctgcgcattg agtttttgat tcagcaactc accgttaatt tacccatcgt
     4861 tgaccacgct ggcgcgctgc atttcttccg taatgtcagt gaattactgg atgttttcga
     4921 gcgcggcgaa gtccgcactg agctgttgaa agaacttgac cggcagcaac gtaaactcca
     4981 gacctggatt ggcgtgcctg gcgtggacca gagccgtatt gaagcattaa ttcagcagtt
     5041 aaaagcggcg gggagcgtat taatttccgc gccgcgtatc gggcaatttc tgcgtgaaga
     5101 tcgtttgatt gctctggtgc gtcagcgact gagcatccca ggcggctgtt gcagctttga
     5161 tttacctaca ttgcacattt ggctgcatct accccaggcg cagcgcgaca gccaggtaga
     5221 aacctggatt gccagcctga acccgctcac ccaggcactt accatggtgc tggatttaat
     5281 tcgccagtcg gcccccttcc gtaaacaaac cagcctgaat ggtttttatc aggataacgg
     5341 tggcgatgcc gacttgctgc gcctgaatct gtcgctcgat tcacagcttt atccgcaaat
     5401 ttccggacat aagagccgtt ttgccattcg ttttatgccg ctggacagtg aaaacggaca
     5461 ggtaccggaa cgtctagatt tcgaactggc ctgttgctaa ggagtgaaaa tgtcagaaac
     5521 tattacggtg aattgcccaa cctgcgggaa aacggtggta tggggtgaaa tcagcccgtt
     5581 tcggccattt tgctccaaac gttgtcagct gatcgacctc ggagaatggg ctgctgaaga
     5641 aaaacgaatc cccagcagtg gcgatctttc cgaaagcgat gactggagcg aagagccaaa
     5701 gcagtgacat tggtctgatg ccgcacatag gcctgataag acgcggacag cgtcgcatca
     5761 ggcatcttgt gcaaatgtcg gatgcggcgt gaacgcctta tccgacctac agactacaga
     5821 cgcttaagct tcgcaattac cggttcattg gctggcggaa aatcatcggc attaagaccg
     5881 accagcgaca tccactcacc gggttgccct tctttacccc acggcacccc ttcccaactt
     5941 tcgaccagcc agaaccacag agttatatgc ctgtccggga attcatatgc cagtttttca
     6001 aatagcgaaa aatgttgtgg ggtaatcccg acctcttcct gaagttcacg caccaccgcc
     6061 tgttccggcg tttcgcccat ttcaatttta ccgccgggaa actccagttt attcgccatg
     6121 tgcgcatctg ctgcgcgacg cgttataaag atttcattgt tctcgttgcg aataatacct
     6181 accgcaattt gcagcttttt cattgtttta aacctataaa aaaggcgcag aatcctgcgc
     6241 cttttgcttc aacagttagc ttttattgca ggcggccatg acactgcttg tattttttac
     6301 cagaaccgca cgggcaagga tcgttacgtc ctactttgcg ctcaccggtt tgcgccgcca
     6361 gtgcagctgc ggctgcagag tcgtcatcct gatggctaag ctgctgcatt tgcgctaaac
     6421 gctcggcttc catacgacgc tgttgttcca gctcctcaac ctcttcaggc atacgtacct
     6481 gaactttgct cagcgtactg ataacttcat atttcaacga ctccagcatc gctgcaaaca
     6541 tggagaacga ttcacgtttg tattcctgct tcggatcttt ctgtgcatag ccacgcaggt
     6601 ggataccctg acgcagatag tccatcgctg ccaggtgctc tttccacagg gagtcaagcg
     6661 tttgcagcat gacgcctttc tcgaagtgac gcatcatctc agcaccaacc acttcttctt
     6721 tacgctgata cacttcgatg gactgcgcca gaatgcgctc acgcagcgtc tcttcatgca
     6781 gttctggttc tttatccagc cactcggcaa ttggtaaatc gaggtcgaaa tcgttcttca
     6841 gacgttcctg cagccccgga atatcccaca tttcttccag cgactgtggc ggaatgtagg
     6901 catcaatggt ggctttgaac acatcttcac gaatgctgtt gatggtttcg ctcacatcgc
     6961 tgacatccag cagttcgtta cgctgggagt aaatggcgcg acgctgatcg ttagccacgt
     7021 catcatattc cagcagttgc ttacgaatgt cgaagttacg actttcaact ttacgctggg
     7081 cgttggcaat cgctttagtc acccacgggt gttcaatggc ttcgcctggc ttcataccca
     7141 gtttacgcat catgccggat actcggtcgg aagcaaaaat acgcatcagc gcatcttcca
     7201 tcgacaggta gaaacgggaa gaaccagcat ccccctgacg accagaacga ccgcgcaact
     7261 ggttatcgat acgacgggat tcgtgacgct cggtaccgat gatatgcagg ccacctgctt
     7321 ccagtaccgc atcgtgacgt acctgccagt cggctttaat tttttcaatt tgctctgcgg
     7381 tcggattttc cagcgcggca acttctgcct gccagctacc accgagcaca atatctgtac
     7441 cacgacccgc catattggtc gcgatagtca ccgcagccgg ataacctgcc tgagcaacaa
     7501 tcgccgcttc gttggcgtgg aatttggcgt tcaggacgtt gtgcttaata ccggctttgg
     7561 tcagttcgtt tgacaccagc tccgattttt cgatggagat agtacccacc agcaccggct
     7621 ggcctttcgc agtacgttct ttgatatctt caatgatcgc ctgaattttt tccgcttcag
     7681 tcatgtagac caggtccggc agatctttac gaatcattgg acggttggtc ggaacaacga
     7741 cggtatccag cttgtagatg gagctaaatt cgaaagcttc ggtatcagca gtaccggtca
     7801 tccccgccag tttttcatac agacggaagt agttctggaa ggtgatcgaa gccagcgttt
     7861 ggttttcgtt ctggatctgc acaccttctt tcgcttccac agcctggtgc agaccatcgg
     7921 accagcgacg gccctgcatg gtacgaccgg tgtgttcgtc aacgatgata acttcaccat
     7981 ctttaacgat gtagtcgacg tcgcgggtaa acagcgcatg agcgcgcagc gccgccgtta
     8041 cgtggtgcat cagcatgatg ttggccggag agtacagaga ctccccttca tccatgatgc
     8101 cctctttcac cagcagttct tcaatcagca ccagaccacg ttcggtcagg ttcacctggc
     8161 gagatttttc gtccaccgag aagtggcctt cgccctggaa ggtttcggag tcttcttttt
     8221 cctgacggat caggtgcgga ataattttat tcacgcgttt atacatttcc gagctgtctt
     8281 ctgccgggcc ggaaatgatc agcggtgtac gcgcttcatc gatcaggatg gagtccactt
     8341 cgtccaccag cgcatagtgc agtttacgct gtacacgttc ttcagggctg aacgccatat
     8401 tgtcgcgcag gtagtcaaag ccgtattcgt tgttcgtacc gtaagtgatg tcagctgcgt
     8461 aagcttcgcg ctttgccggt gctggcatgc ccggcaggtt gataccgaca gtcaggccaa
     8521 ggaattcaaa cagcggacgg ttgttttcgg cgtcacgttg cgccaggtag tcgttgacgg
     8581 taactacgtg cacgccttta ccggttagtg cgttcaggta agcaggcagc gttgcggtca
     8641 gggttttacc ttcaccggta cgcatttcgg cgatgcagcg ttcgttaaga accataccgc
     8701 cgagtaactg aacgtcgaag tgacgcatac caaagacgcg cttacttgcc tcacgtacca
     8761 cggcgaaagc ttccgggatc agattttcca gcacttcgcc tttttccaga cgtgcacgaa
     8821 actctgcggt tttccctttc agttcttcgt cggagagttt ttccatctcc ggttccatgg
     8881 cattgatgat gttgaccact ttgcgcatcc ggcgcagggt gcgatcgtta cgactaccga
     8941 aaactttagt taacaatttg attagcataa taaaatctca aacgccccgc gttgcggagt
     9001 taataaaatg aagtaaaggt ttattgttgt taggtgaggc gttgagggcc agcacggatg
     9061 ccttgcgcct ggcttatcca gacgggcgtg ctgaattttg cttgtggggt aaaatgcgca
     9121 taatcaatgc gataaccctt ttcagacggc gtgccttcct gggtcagcag cgcgctgagc
     9181 gtatccagta atgcaagatg ttgcgcctga agaggcaaag attcttcagc aacgggcagt
     9241 gtttgcggtg ccattgcgaa agaaagatga cggattaccg tgcgaatggc atattgatgc
     9301 cagtaatcaa cggaatagtt cgaattcggg cggcgtgtgt tcgcttccag caaggccaat
     9361 tgaccaaagt taactttggc tgaaggctcg tggttgcggg ttgtcgcttt tgcgggcacg
     9421 tttggttcgg cggcgttgct gagcgcaggc aaacctaaac tcgccgcgac catccctaat
     9481 aagagatgcg gccagaagta gcgtttacca aactgtcgcc agcgcgtcag tattccactc
     9541 acgttattgc catcccgatc gaattcaaac gtatttagcg cacaaatctt atcattaaag
     9601 cctgaagtcc acagcatgaa tgaagataag ttgtgcgtaa aagtgcttaa gaaaacagcg
     9661 ttcgcaccgt tttttatagc tcttataaaa ttcaaaaaaa aacgactggt tcacctggcc
     9721 ggagagagtg ccagatttgc cagtcgaatt ttatacgaca gtataaatgt cgttatgcca
     9781 gtacagctga aggcgctttg aaggtcaacg gcagttctgc gtcgtcctgg aaggtcacat
     9841 attcccaagc ttcctgtttc gccaggacag cctgcagcag tttgttattc agtgcatgac
     9901 cggatttata agcgataaat gcaccaataa tattgtgacc acacatgaac aagtcaccga
     9961 tcgcatcgag cattttgtga cgcacaaatt cgtcttcaaa acgcaggccg tcttcgttca
    10021 gtacgcgata atcgtcaaca acgatggcac aatcgaagct gccgcccagg cacaaaccac
    10081 gggactgcag atattcgata tcacgcatga aaccgaacgt acgcgcacgg ctgatctggc
    10141 gcataaacgc atcagcggag aagttcatcg catagcgctg gttgctggaa tcaatagccg
    10201 gatggttaaa atcgatggtg aaatccagcg aaaaaccatt gtacggctta aattcagccc
    10261 acttatcgcc atcttcgaca cgaacagtct ctttgatgcg aacaaatttt ttggcgcagt
    10321 tcaactcgtc gataccggcg tcaagcagca ggtatacaaa cggagcggcg ctgccgtcca
    10381 tgatcgggat ttccggcgcg ttaacttcga taacaatgtt atcgatgccc aggcccgcga
    10441 gagcagcatt gaggtgctct acggttgaaa tccgtacatc atgctcgttg accagacacg
    10501 tacagagcat ggtatcacgc acagatttgg catcggccgg gaaatctacc ggtggattca
    10561 agtcggtgcg acgatagatg accccggtgt tggccggcgc agggcgtaac gtcagagtga
    10621 ctttcttgcc ggtatgtaaa ccgacacccg tcgcctgaac gatacgttta agtgtccttt
    10681 gtttgatcat cgtattatct cgccaaatta cctatccaac cgaagtgtac tatacattcg
    10741 gcgggccagt ttagcacaaa gagcctcgaa accaaaattc cagtcaattc ttaatcagct
    10801 tgcttacgca ggaatgctgg gatatccaga taatccggct ctttcgcagt ttgcggcgca
    10861 ttgtcattca cgactttagc aaccggcttc tgctcctggg tcagcggagc catcccatgc
    10921 tgctggtagc gatccatcac tggctgctga acctgcttat tggtcaccag agtgatttca
    10981 ggacgtttgt ccatgccgat acctgtcgca acaacggtta cgcgcagctc gtcattcata
    11041 tccgggtcaa gagaagtacc gataaccaca gtcgcgttgt cggaagcaaa tgcacggatg
    11101 gtgttaccta ccgtttcgaa ctcatccaga cgcaggtcga agcccgccgt gatgttaacc
    11161 agcacgccgc gcgcgccaga caggtcgata tcttccagca gcggagaaga gatagccatt
    11221 tcagcagctt cttccgcacg gtcttcaccg ctcgccacgc cagaacccat cattgcgtag
    11281 cccatctcag acattacggt gcgtacgtct gcaaagtcca cgttcatcaa acccggacga
    11341 gtaatcagtt cagcgatacc ttgcacagcg cctttcagta catcgttcgc tgcgccaaac
    11401 gcatccagca gggagatacc gcggcccaga actttcagca gtttgtcgtt cgggatagtg
    11461 atcagagagt ccacatgctt ggacagttca gtgatcccct gctccgcgaa tgccatacgc
    11521 ttcttgcctt caaagttgaa aggcttagtg acgacagcaa cggtcaggat acccaaatct
    11581 tttgccactt cagcgacgac tggtgctgca cctgtaccgg taccaccacc catacccgca
    11641 gcaataaaga ccatgtctgc accttccagc gccgcacgca atgcatcgcg atcctcatca
    11701 gccgcattgc ggccaacttc tggattagcg ccagcgccca gtcctttggt gataccgcta
    11761 ccgatttgaa tcgtctgtcc aaccgctgtt ttacgcagcg cttgtgcatc ggtatttacc
    11821 gcgaagaatt caacaccttc aatgcgctcg cgcaccatgt gttcaacagc attaccgccg
    11881 ccgccgccga cgccgatgac tttaatcacc gcgtcattgg taagttccat tggttcaaac
    11941 atagtttctc tccgatttgt gcctgtcgcc tgaggccgta atcatcgtcg gcctcataaa
    12001 aattaaaact cttttcgcag ccaactattg agtcgcttga tccacgagcc aactgatgct
    12061 gtaacacgtt tttctacttc agcttcaccg ttaagatgtg actctttccc atagtgaagc
    12121 aatcccaccg ccgtcgaata atacggctcc tgagcataat ccgttaaacc ggtaatgttc
    12181 agcggcgcgc cgatacgcac ttgcgtatga aacacgcgct gagcacaggc tgcaagacct
    12241 tcgatctgcg ctgcgccacc ggttaataca atgcctgccg ccaggtgatg tttaacccct
    12301 tgttggcgaa gcttttcctg caactgcaat atctcttcgt tgaccaggtt gagcagctcg
    12361 gtatagcgcg gctcgatcac ctctgccagg gtctgacgtt gcagactccg tggcggacga
    12421 ccgcctacgc tcggcacttc cacgctctca tcttttccaa cgatggaacc cagcgcacaa
    12481 ccgtggcgaa ctttaatcgc ttcggcgtcg cttggcggcg tgccaaaggc gtaagcgata
    12541 tcactggtca cgacattgcc agcataagga attaccttag tgtggcgcaa tgccccaccg
    12601 gtataaacgg cgatatccat tgtaccacct ccgatatcga cgacgcagac acccagttca
    12661 cgttcatctt ccgtcaatac cgaataactt gatgccagtc cggcaaatat cagttggtca
    12721 actttcagcc cacaacgttc aaccgctttg acgatgtttt tcgccatatc gttgtgacat
    12781 gtgatcaggt gcacttttgc ctgcatccgc acgcccgaaa gtcctaccgg attcttgatc
    12841 ccttcctgat agtcaatcgc atactcttgc gggatcacat gcagcacacg atgttcatcg
    12901 cgcacacgca ccgatttcgc ggtatggacg acgttttcca catcttcttg cgtcacttct
    12961 tcttcagaaa taggcaccat accaatttca ttctggcagc tgatgtgctt accagaaagc
    13021 gccagatata ccgaagagat ctgacaatct gccatcaatt ctgcctggtc aatggcgcgt
    13081 tgtacgcact tgaccacgga ttcgaggtcg ttcaccccgc ctttatccat accacgtgac
    13141 gggcagctgc ccacgccaat gatattgacc ataccgtcgg gcagaacttc ccctactaaa
    13201 gcggcaacct tcgcggtacc aatctccagt cctactacca gttttctgtc cgtcgccttg
    13261 atcattgttg ttctgcctgt gcctgatttt gttgctgagt agattcctct ggcggcaagg
    13321 gcgcccagcc tactgccgct ccagagtcat aacgcaaatc aacgtagcta atccgtttgc
    13381 catcggtttg cgccagctgc tgtaaaaccg gataaagttc tacaaagcga gccaaacgtt
    13441 tcatcgtatc gccccggcca agattgagct taatatcgtt attcagcgtc aactgccagg
    13501 aacgccgcgc ggtcatcgcc gtttccttca gagtaaatct gtcctttgcc agcatctgcc
    13561 ccatttcgcg atagccctgc aacacttcat tggcgctgcc ttccgggcca tacagcattg
    13621 gaagcacctg cttgctggtg cgatctggcg gcacgctgaa ggtgtttcct tccgcgtcta
    13681 ccatatgttg atcattccac cgcgcaatcg gcacatattc aaccagatga atcttcaatt
    13741 catcaggcca ctgctttctg acgctcacct gcttaatcca cggcaggcgt tgttctattt
    13801 gcgtctggat gatgttgaca tcctgggtca taaaggtacc cggctcaccc aatgccagga
    13861 tcgactgccg gatatcgtca ttacgcgtgt aatggcgttc accggtcaac accagctttg
    13921 agagcggcag gcgttgcgca tcttccatcc agcccaacac gacccagccg ctcaccaaca
    13981 ctgtcgttaa aacggtcagc aggaaaagga tccccgccag acgcgttcca ttattgcggc
    14041 gagaagaaac ctcttcttcg ctgtttcgcg tgttcagagc agcctgcgac atattagtcc
    14101 gccagttcca gaattcgtac taccaactgc gagaagctca tacctgcctg acgtgccgcc
    14161 atcggcacca ggctgtggct ggtcataccc ggtgaggtat tggcttccag cagataaaac
    14221 tgtccatcgc tgtccagcat aacgtcaata cgtccccatc ctttgcaacc taacgtcgtc
    14281 catgctttca gcactaatgc ctgcaaattg gcctcttgtg acgcatccag acctgcgggg
    14341 cagaaatact gtgtctcatc agagagatac ttcgcctcat aatcatagaa ggttccggac
    14401 ggttgaatac gtattgacgg taaaatttct tcaccgagta tcgcaaccgt gaactccggc
    14461 ccacttagcc atttttcaat caatacttct tcatcgtgct gaaatgccaa tcttaatgca
    14521 tcttgtagag cattttctgc tactactttt gacattccca cactggaacc ttcgcggctc
    14581 ggcttaacga taaccggcaa acccagagca gaaatttctg ctaactgctt atcgctcagg
    14641 cctttttcaa actctgcgcg ggttaacgct acccacggcg cgaccggtaa accggcacct
    14701 tgccacagca gtttgctgcg tagtttatcc attgaaagcg cagatgccat cactccgctg
    14761 ccggtataag gcaagcccat cagctcgagc atgccctgca gcgtaccatc ttcaccgccg
    14821 cgaccgtgta gcgcgataaa cactttctga aaacccatcg acttcagttg cgtcacgtcg
    14881 acttctttcg ggtcgacagg atacgcatca ataccacctt cacgcagccc ggctaacacc
    14941 gctgcgccag aattcagaga aacttcccgc tcagcggaag tcccgcccaa caggaccgcg
    15001 attttatcag tcatgttgtt cttcctccgg agtttgcggc ttcagtttga tttcagctaa
    15061 agaacgggca atttttccga tattaccagc cccctgaacg agaatcaggt cgttaccggt
    15121 taataccggt gccagcatct cggctacctg cgcagggtcc ggcaccagaa tgggatcaat
    15181 tttcccacgt ccacgaattg tgcgacacag cgaacggctg tccgctcccg gaattggcgc
    15241 ttcgccagcc ggatacactt ccagcatcaa cagggtatca acctgcgtca gcacattggc
    15301 gaaatcatca tacaagtcgc gcgtacgggt aaaacggtgc ggctgaaaca gcattaccag
    15361 gtttttatcc ggccagcctg cgcgcgccgc tttaatggtg gcgtcaactt ccgtcgggtg
    15421 gtggccgtag tcatcgacca gcattgccgt accgctttta ccattcactg gctccagcgg
    15481 gaattcaccg aggaaatcaa aacggcgacc agtccccagg aagctctcca gcgcacgcaa
    15541 aatagcctcg tcgtcaattc cctcttccgt agcaaccgca accgcagctg cggcgttcag
    15601 cgcgttatga cggcctggcg cattcagggt gacgcgcatc ggctctttgt cctggcgcag
    15661 cagcgtaaag tgcccctgcg ggccaatctg ctgataatct tctacacgca cgtcggcatc
    15721 ttcgctgaag ccgtaagtcg tggtctgacg tcccacacgc ggtaacaatt cgcggatcac
    15781 cggatcatca acacacatca ccgcacgacc gtaaaacggc aggttgtgca gaaaattaat
    15841 aaaagtctgt tttaaattct caaagtcgcc ctggtaggta tccatgtggt cggcttcgat
    15901 attggtgaca atcgccacca tcggttgcag atgcaggaac gatgcatcac tctcatctgc
    15961 ttcggcaatc aggtaccgac catgccccaa acgcgcatga acccccgccg cttttaccag
    16021 cccgccgtta acgaaggttg ggtcgagccc cgcttctgcg tagatgctgg aaaccatcgc
    16081 ggtggttgtc gttttgccgt gcgttcctgc aatggcgatg ccatgacgaa aacgcattaa
    16141 ctcagccagc atttcggcac gacggatcac cggaatacgc gcttcatgag ctgcgacaat
    16201 ttccgggtta tcggcagaaa tggcgctgga aacaacgacc acgctggcat cacgtacgtt
    16261 ttccgggcga tggttgaaat aaatcgtcgc tcccagattc attaactgct gcgtgaccgg
    16321 atttggcgct aaatcggaac cactgatctg ataaccttca ttggccagaa cttcggcaat
    16381 accgcccata ccggcaccac cgatgccgac aaaatgtatg tgccgaacgc gacgcatttc
    16441 gggcacgatg gaacgcagtt ttgccaattg ttgtgtattc attctttacg ccattaactt
    16501 cttaaattca tacgatgcaa aaggcatcgc tacaattacg cccgggcagc ccggctcact
    16561 tcatttgcca ctcgctcggt ggcatccgga atggatgcag cgcgggcgcg ttctgccatg
    16621 gttaataagg tttctcgcga ccacccggcc agggtgttgg cgacagcatc cacgctaagc
    16681 tgtggctgct cgataatttt ggctgcgccc gctttttcca gcggtagcgc attccagtat
    16741 tgctggcggt ctttatgttg aaacggcaca aacaacgccg gtagtcctgc cgcggcgatt
    16801 tcactcaccg ttaaagcacc ggagcggcaa acgacgacat ccgcccacgc atacgccgcc
    16861 gccatatcat caataaattc cgtcacttta tgctgcggtt gccccgcttc ggcatacgcc
    16921 tgttcaacgg attgttgcga acctttaccg ctctgatgcc agatagtgac tgaatcaccc
    16981 agtttcgcag caacctgcgg cattgtctgg ttaagaatgc gtgcgccctg ggaaccaccc
    17041 actaccagca cacgaaccgg accttcacgt ccagccaaac gttgctgcgg caacggcagc
    17101 gccagcacat cggtacgcac cgggttaccc accacttccg cattagggaa agcgcctgga
    17161 aacgcctgca tcactttggt ggcaatcttc gccagccatt tattggttaa gcccgcaata
    17221 ccgttttgtt catgaagtac aaccggaatg cctaacgacc acgcggccag accacctgga
    17281 cctgatacat agccgcccat accgagtacc acgtcaggtt tgtacgcttt cataatcgcc
    17341 cgcgcctgac gccaggcgtt gaagatacgc agcggcgcag ctatcagtgc ttttatacct
    17401 tttccacgca gaccagagat acgaatgaaa tcaatttcga tgccatgttt tggcactaag
    17461 tccgcttcca tacggtcggc agtccccagc cagcgaactt gccaaccctg agccattaga
    17521 tggtgcgcaa ccgccagtcc cgggaataca tgtccaccgg ttccgcctgc catcaccatt
    17581 aatcgctttc cttgaccact catcgtgaac ctcgtacaaa cgcctgcgct ttctccagac
    17641 gcgtttcata atcaatacgc aacagcatca tgatggctgt cgacataatc agtaagctcg
    17701 aaccaccgta actgatcagc ggcaatgtca gacctttggt cggtaacatc cccgccgccg
    17761 cgcctacgtt aaccagcgcc tggaagctaa accagatgcc aatagaacag gcgagaaaac
    17821 cggaaaaacg gtggtcaatt tctaacgctt tgcggccaat cgacatcgcg cgaaaagcga
    17881 cgaagaatac cattaaaagt gccagcacca caccgacata ccccagttct tcgccgataa
    17941 tggcgaaaat aaagtcagtg tgcgcttccg gcagatactc cagtttttgt accgagttac
    18001 ctaaaccttg tccccaaagt tcgccgcgac caaacgccat cagcgattgc gttaactgat
    18061 agccgctgcc aaagggatct tcccacgggt tccagaatgc ggtaacacgg cggatacggt
    18121 acggttcggc gagtatcagc aacacaaccg ctgaaatgcc cataccgata atggcaatga
    18181 actgccacaa tttcgctccc gccaggaaca acatcgccag cgtagtcaca aacaccacca
    18241 ccaccgtacc aaggtctggc tgtgccagca gtaacactgc caacaccaga atcacgccca
    18301 tcggtttcag gaagccgcgc aggttattac gtacttcgtc gcctttacgc accagatagt
    18361 tggcgatata acaaaacagc gacagttttg tcagctccgc aggctggata cgcagcaaac
    18421 cgagatcgat ccaacgcgat gcccctttaa ccgagctccc cactaccagc acgatcatca
    18481 gcaggatgat agatccgagc agcatcgtgg cactgtagcg ttgccagaac tccatcggca
    18541 gacgcagcgt aatgatcgcc agaatgaatg ccaggatcag atagacgcca tcacgcttcg
    18601 cgaagaagaa cggatcgttg gttaagcgtt gccctatggg cattgacgcc gaggtcacca
    18661 tgataaagcc aatcgccgca aggccgaagg tcagccacag taaggtgcga tcgtacatga
    18721 tcaggctgtc ggtatctttt tcccgcgagc ccatcaccca gccttttagc gccgtggaga
    18781 tccagaccag gatactgaat cctggcaggc gcggcatttt caggcgaggg agagataaac
    18841 gcatcaacct aactccttcg ccagacgggc aaactcattg cctcgttgtt caaagttctt
    18901 gaactgatca aggctggcac aggccgggga gagcagaacc atatcgcccg gctgaacacg
    18961 cggagccagc aagcgcatcg cctgttccat agtttcggtt tgttctgcca cttccgggcg
    19021 tagcgccgcc agctgcgcac cgtcacgacc aaaacagtac aaacgcacgt tgtcgccatt
    19081 cagatagcgc gccagtggac tgaagtccgc cgatttacca tcgccgccca gcaacaaatg
    19141 cagcgtgccg tctacctgca agccattcaa cgccgcttcc gtactgccga cgttagtggc
    19201 tttcgaatcg ttaatccagc gtacgccatt gtgctccagc accacttcaa agcgatgcgg
    19261 cagaccggtg aaggtggtta acgctttcag gctgctggcg cgcggcaaac cagctgcgtc
    19321 tgccagtgcc agtgctgcca gcgcattggt gtagttatgc tgcccggaaa gtttcatctc
    19381 tttcacgttc agcactttct cgcctttcac tcgcagccag gtttctccct gctgatgatt
    19441 caggtgatag tcacccatgt tgacgccaaa gctgacgcag cgttcatcag caccgcgaat
    19501 cggcattgtt aaggcatcat cagcattgac gacacaaact ttcgcatttt cgtaaatacg
    19561 caattttgcc gcacggtatt gctgcaaacc aaacggatag cggtccatat gatcttcagt
    19621 cacgttcaga atggtcgccg ccaccgcctg taagctggaa gtggtttcca gctggaagct
    19681 cgacagttcc agcacgtaca gttcgcactc agcatcaagc agcatcaacg ctggcaggcc
    19741 aatattgcca cccacaccaa cgttaacccc cgccgctttc gccatttcac ccactagcgt
    19801 ggtgaccgtg cttttgccgt tagaaccggt aatcgccaca atcggtgctt gtgcttcgcg
    19861 gcagaatagc tcgatatcgc caacgatttc gattccggca tcagcggcag cgcttaagga
    19921 tggatgcgcc agtgcaatac cgggactggc gacaatcaga tctgccgcca tcagccattc
    19981 atcattcaga ctgcccgtgt ggcgttctac ggcttcgggt aatttatcca ggccaggcgg
    20041 tgtcatacgc gtatccataa cgcgcggcgt cacaccgcga gcgaggaaaa agtccacgca
    20101 ggaaagcccg gtgaggccca ggccgataat gacgacattt ttaccctgat aatcagccat
    20161 gattaacgta ccttcagcgt tgccagacca atcagaacca gcatcagcga aataatccag
    20221 aaacgcacaa tgacgcgcgg ttccggccag cctttcagtt catagtggtg atgaatcggt
    20281 gccatgcgga aaatacgttg tccgcgcagt ttaaaggagc cgacctgcag gatgacagaa
    20341 agcgtttcta ccacgaacac gccccccata atcaccagca ggaattcctg acgtagcagt
    20401 acggcgataa tgcctaacgc accacctaac gccagcgaac ctacatcgcc cataaagacc
    20461 tgcgccggat aggtgttaaa ccacaggaag cccagtcctg ccccgactat cgcggtacag
    20521 acaataacca gttccccggc gtgtcgcaga tacggtatgt gtaaatagct ggcaaagttc
    20581 atgttgccgg tcgcccacgc caccagcgca aaaccaccgg cgacaaatac ggtcggcata
    20641 attgccaggc cgtcgagacc atcggtcagg tttaccgcgt tgccagtacc cacaatgacg
    20701 aagtaagcca gcagaatgta gaacagcccc aactgcggca tcacatcttt aaagaatggg
    20761 accaccagct gcgttgcggg cgtgtctttg ccggcaaggt acagggcgaa ggcgacaccc
    20821 agcgcaatga ccgacatcca gaaatacttc caacgagcga tcaacccttt ggtgtcttta
    20881 cgcaccactt tgcgataatc atcaacaaag ccaataacac cgtaacctac cagcaccacc
    20941 aacacgcacc agacgtacgg attggacggg taagcccaca gcagtacgga gatcacaatc
    21001 gccgtcagga tcataatccc gcccatggtc ggcgtaccgc gcttgctgaa gtgtgattca
    21061 ggaccgtcgt tacgcaccac ctgaccaaag gaaagttttt gcaaatgagc aatcatacgc
    21121 gggcccatcc acaatgagat gaacagcgcg gtcagcaggc tgacgatggc gcgaaacgtc
    21181 agataggaaa agacgttaaa gccggaataa tatttgacca aatgttcggc cagccaaact
    21241 aacatgtccc attctcctgt aaagcgcgta ctacctcttc catggcggca ctacgtgaac
    21301 ccttaactaa aatcgtaatt acctgttgct cagcaatcag tgatttaaga cgcgtaatta
    21361 acgcagtttt atcagcaaaa tgttcgccaa cgccgctggc ggtgctgata gcatggcttt
    21421 gtttacccac gcttaacacg cggtcaatac cagcagcttt tgccgcctcg cccacctgta
    21481 catggcaggc ttcgctttca gcgcccagtt ccgccatatc gcccaccacc agcacgcggt
    21541 agcccggcat ttcagccagt acctgaacag cggcggtcat tgaaccgaca ttggcgttgt
    21601 aggagtcgtc gagcagcaac tggttttctg ccagttggat ggggaacaga cggcctggaa
    21661 cagctttcag atttgccagc cccgctttga tagcatcaag cgttgcgccc acggacatgg
    21721 agagcgcagc ggctgccagc gcattcgcaa tattgtgacg ccccggcaac ggcagcagaa
    21781 catcgacgct accggttggg gtttgtaggg taaattccgt accgtgcgag gtcacatgga
    21841 tattggtggc ggtgaaatcg ctgttggcgg cattgggtga gaaacgccac actttgcgtg
    21901 agccaattac gctctgccag ttcagccagt cgttgttgtc ggcgttcata atggcgatac
    21961 cgttttccgg caggccgcta aagatttcac ctttcgcttt cgcgacaccc gcaagcgagc
    22021 caaaaccttc cagatgcgcc gctgccaggt tgttgaccag cgcagcttcc gggcgagtca
    22081 gactcacagt ccaggctatt tcgccctgat ggttcgcgcc aagttcaata actgcgtaat
    22141 cgtattccgg cgttaagcgc aacaacgtca tcggtacacc gatgtcgttg ttgagattgc
    22201 ctgccgtata aagcgtgttg ccgcactggc ttaaaatcgc cgccgtcatc tctttaacgg
    22261 aggttttgcc ggaggaacct gtcagagcaa ccacgcgcgc cggaacttgc tggcgaaccc
    22321 atgcagccag ttcaccaaac gccagacgcg tatccttgac gattaactgc ggcaggtcga
    22381 tatccagcgg acggctgacc agtagtgcgc ccgcgccgcc agctttcgcc tggtcggcaa
    22441 aatcgtgggc atcaaaacgt tcgcctttca gggcaacaaa caggcagccc ggcgtcagtt
    22501 ttcgggtatc agtggttaca gcatcaaggg tgatatctgc accttgcagt tcaccattga
    22561 gaatgtcggt aagttggcta agggttacgc taatcatgca atcaccccca gcagacgcgc
    22621 caccgtgacg cgatcggagt agtccagacg ctgattgcca acaatctggt aatcttcatg
    22681 gcctttgccc gcgaccagca ccacatcgtt ctctttagcc tgcataacgg cgcaagtcac
    22741 cgcttcagca cggccttcca tcactttggc atgtccggca tctaacattc ccgccagaat
    22801 atcgttgatg atggcacgcg gttcttcggt acgcgggtta tcgtccgtca ccaccgccac
    22861 gtcagcaaac tcttcggcaa ttgcgcccat cagtggacgc ttacctttat cgcgatcgcc
    22921 accacagcca aagacacacc acagcttgcc cgcacagtgc agacgcgccg cctgtaaggc
    22981 tttttccagt gcatccggcg tatgcgcgta atccaccacc accgtcggtt tgcctggcac
    23041 agtgaacact tccatacgtc cgcaaaccgg ttgcagacgc gcggcggttt tcaacagatc
    23101 agccaatgga tagccgagtg ccaacagtgt cgccagcgcg agcagcaggt tgctgacgtt
    23161 aaaagcgcct atcagatggc tttcaatttc gccatcgccc caacttgagc taaagcgaat
    23221 cgtcacaccg ctgtcgtgat agttcacgtc gatcactttt aaccagcgtc cgtgacagtt
    23281 cggattaata tgatcttcca ttgataccgc aaccgcgtcc ggcagctttg ccagccaacg
    23341 gcggcccact tcatcgtcgg cgttaataat cgcctgaccg caatgatgtt cagaataaag
    23401 cagccatttc gcagcttcgt agtgttccat atcaccatga taatcaaggt gatcgcggct
    23461 taagttggta aagaccgacg ccgcaaattt caatgccgcc acacggtgct gtaccagccc
    23521 gtgggaggaa acttccattg cgcaaaacgt cgcgccctgc tccaccagcc ccgccagctc
    23581 atgctgaaca tcgactgccg aaccggtggt attttctgtc gggatcactt tccccagcag
    23641 accgttacca acggtgccca ttaccgcgct ggtttcgcca agcagttggc tccactgcgc
    23701 cagcagctgg gtagtcgtgg ttttgccgtt ggtgcccgtt acgcctacga gacgtaaatt
    23761 gtcagagggt tcatggtaaa agcggcccgc cagtgcagat aaacgctcgt tgagctggct
    23821 gagatagatg accggtacgc cgtgcatttc acggatttca ccgtcggtcg cctcatcttt
    23881 cgcctctgca ataatggcag ccacaccttg cgctatcgcc tgcgggatat atcgacgccc
    23941 gtccgcctga tgacctacta cagctacaaa gagatcgccc gccgcagcca cacggctgtc
    24001 gagtgtcatc tctcgcagtg ctcgcgaagg tgcgtctggc acccacggag caagaaggtc
    24061 gcgcaaatta cgatctgcca cctgtcccct cgccttgatt aatcacaaat tcatttttat
    24121 cgcccgttgt cagcgcatcc ggctcgatgt tcatggtacg caatacgccg cccatgatgg
    24181 caccaaagac cggcgcggaa acggcgccgc cgtagtattt acccgcctgc ggatcgttga
    24241 taacaacaac cagcgcgaag cgcggctgac tcgcaggcgc aacgcctgcg gtataagcaa
    24301 tatatttatt gatgtagcga ccgtccggcc cgaccttttt cgcggtaccg gttttaatgg
    24361 cgatacgata gcctttaatc gccgccttca cgccgccgcc gcccggtagc gccacgcttt
    24421 ccatcatatg caccacggtg cggacaatgg attccgggaa gacacgttca ccgggaaccg
    24481 gggggtcaac tttggtaatc gacagtgggc gataaatgcc gtagctgccg atagttgcgt
    24541 agactcgcgc taactgtaac ggtgttacca ttagcccgta gccgaaagag aaggtggccc
    24601 tctctatgtc agaccaccgt tgtttttgag gatataagcc actgcgttct ccgaccaacc
    24661 ccaaattggt cgcttttccc agtccaaaac gtgagtaagt atctactaac gctgaggacg
    24721 gcatcgctaa cgccagcttg gaaacaccga cgttactcga cttctgtaat accccggtca
    24781 gggttaattc gctgtagcgt gccacgtctt tgatttcgtg gccgttaatt cgataaggaa
    24841 tggtattgag taccgagttt tcccgcacca cgccacgttg caacgcggtc atcaccacca
    24901 tcggtttaac cgttgagccc ggttcaaaca cgtcggtgat ggtacggtta cgcatcgcct
    24961 ctttcggcgt gccgctcaga ttgttagggt tgtatgacgg gctgttagcc atcgccagca
    25021 cttcacctgt gttgacatcc accagcacgg cgctaccaga ttcagccttg ttaaaggcca
    25081 ccgcgttgtt cagttcgcga taaaccagcg cctgcaggcg ttcatcaata ctcagcgcca
    25141 ggttgtgcgc tgcctggctg tcagtagaag aaatatcttc aattacgcga ccatagcggt
    25201 ctttacgcac aatgcgctca cccggctgcc cggtaagcca tttatcgaaa ctcttctcaa
    25261 cgccctcaat cccttgacta tcgacgttag taaagccaat gaggtgagca gtcacttcgc
    25321 cggatggata ataacggcga gattcttcac gcagatgaat acccggcagt ttcagttttt
    25381 tgatgtagtc cgccatgtca gggtttacct gacgcgccag ataaataaag cgccctttcg
    25441 ggttggcgtt aatgcgggca gaaagctgat ccagcggaat attgagcgcg ttagccagcg
    25501 ccttccagcg gtcaccgacg ctgataccgc cagcgtcatg cacttctttc gggtcagccc
    25561 aaatcgcttt taccggcacg ctcaccgcta acgggcgacc agaacggtca gtaatcatgc
    25621 cgcgggaggt ggaaacttgc tgaacgcgaa gagaacgcat gtcgccctct ttcaccagca
    25681 tatccgggga gataacttgt aaccacgcta cgcgtccgag cagaaaagcc agcgccagga
    25741 gaatacagcc gcataacaac gcaaaacgcc aactaataaa gttggcatgt tcttcctgac
    25801 gttttggttt ctgcgttttc gccgctgctt tcatgcgtcg cgtttatcct tatttttgca
    25861 ctacgatatt ttcttgtgac ggatcaacat gctgcatttg cagcttttcc gtggcgatcc
    25921 tttccacccg gctatggtcg ccgagcgcat tctcttcaag gatcaggttg cgccattcaa
    25981 tgtctaaagc atctcgctcc agcaccagtt gttcgcgctg agcggtcagt aaacgggtat
    26041 ggtgcgccgt ggttaccacg gtcaccgccg tcaaaataat gcaaatgaac aggcagagtg
    26101 gcagcttccc aaatcgcaaa agatcgtcac cgataacacc aggcaatgca tggcgctcgt
    26161 ggcttcccat cgatccttta actttgctta gagcttctgt cactctgctg atcatgcgtt
    26221 cgtcctctct gcgatacgca gaactgaact acgggcacga ggattctcag ccacctcttc
    26281 ttcgcccggc attaacttgc ctaatgctcg cagctgacgg ccacccagtt ttttgagctg
    26341 ctcttcagtc atcggtaacc ctgccggaac ttgcggaccg cggctgtttt cacgcataaa
    26401 acgtttcaca atacggtctt ccagcgagtg gaagctgatg atcgaaagcc gcccacccgg
    26461 ggccagcacg ttgagcgagc tttttagcgc ctgctctatc tcctccagtt cactgtttac
    26521 ccaaatgcgc accgcctgga aggtacgggt cgcgggatgt ttaaacttat ctttcaccgg
    26581 cgttgcagca gccacgactt ccgccagttc tttggtgcgg gtcatcggct gttcgcggtt
    26641 acgctcgaca atggcgcggg caatgcgttt ggcaaaacgc tcttcaccat aggttttcaa
    26701 tacccaggcg atatcggctt cttctgcggt ttgtagccat tcagcggctg actgcccacg
    26761 ggttgggtcc atacgcatgt ccagcggacc atcgcgcata aaggaaaagc cacgttcagc
    26821 atcatcaagt tgcggtgaag agacgccaag atcgaggaga atgccgtcga tcttgccgat
    26881 aagatcgcgc tcggcaacgt attcgcccag cgcggagaaa ggtccgtgga tgatggagaa
    26941 gcgcggatca tcaatagtct tcgcaacggc gatagcctgc gggtcgcgat cgatcgccag
    27001 caaacgcccc tcttcgccaa gctgcgagag gatcagacgt gagtgaccac cgcgaccaaa
    27061 agtcccatca atgtagatgc catcaggacg gatattgagg ccattaacgg cttcatccag
    27121 cagcaccgta gtatgtttat agttttccat cattttatag agacaagtcc tgcagtcgct
    27181 ccgataagtc tccagtagcc aactgctctg cgtcgatatc ttccttgacc tgttgatgcc
    27241 aagttgtttc atcccacagc tcaaacttgt tgaactgtcc aaccagcatc acttcttttg
    27301 tcagcccggc atgttgccgc agtactggcg cgattaacaa tcgacctgcg ccatccatct
    27361 gacattcgct ggcatgacct aacagtaggc gctgcacacg gcgctcaact gggttcatgc
    27421 tcgacagacg cgataatttt tgctcgataa tttcccattc aggcaggggg taaagcagca
    27481 ggcacgggtg atgaatgtca atggtgcaaa ccatttgacc ggcagcgttc tcaagcagct
    27541 gttcccgata acgggtaggc actgataagc gccctttgct gtcgagattg actaacgttg
    27601 ctccccggaa catgccagcc tcacccctta ttcccacttt gccccacaat ttcccactga
    27661 aaggagttta cggagcggag gaaaagcttg tcaagccagt cacaaggcat accgaggtaa
    27721 aaaacccgat gtttacggct aatatcagcg ttgtttattt tacgtccaac gcgcgaagca
    27781 aattaacgtt atgaatagct gtaagaaaaa atccctcatc gtcatcactg ttttaaaatg
    27841 actcactatt ttccacagca aatataaagt gtcagtttgc gacgcgagcg gcattttagg
    27901 acatatcttc cccggttaac agtccttgtt acgtctgtgt ggcgcttgat gccagcgggt
    27961 ttgcgcaaaa gcaaagaaaa gtgtttgtta aatcggcaat tcatcgccgc atgtaacaaa
    28021 ataatgcaaa aacgcgctgt cgatatcact taattattgt gaatttcagc tggtgagtta
    28081 attaacgggc gggtttaaaa ccatttatca ataatagtca ggaatagtct tatttacttt
    28141 aagcatattg atgtccagtc ccgtactcta cgcgccagag ggaaattcac ctggcgcgta
    28201 tttttgttcg cggcttagct acggctgagc acaccgcggc gatagagatt acgtttaatg
    28261 cgcgttaaac caggttttgg cttacgcggt tcgtccaggc ttgccaggac aatctccagc
    28321 acacgctctg cgacatcgcg gtgacgttga gccactgcca gcaccgggca ctgtaagaag
    28381 tcgagcagtt cgttatcgcc aaaggtggca attgccaggt cagaaggcag tttaccatcg
    28441 cgacgtagcg tgacatccat caccccttgc aacaacgcaa acgacgttgt gaacagcgcc
    28501 tgcggcatca gatgcgtttc cagccatttt tcgaataact gggcagccgc ctcccgctca
    28561 tagctgttgg catacaggaa atgcacttcg cgcggatcat ctttccaggc agtacggaaa
    28621 ccttgttcac gcaggaagct gacagaaagc tccggcagcg cgccaagata aagcaccgtc
    28681 tcggcgggaa acttacgtaa ctcttccgcc agcatttcgg catcatcctg atcggcacca
    28741 accacgctgg tgaaatgttc acgatcgagg gcgcggtcca gcgcgacaat cgggaacggg
    28801 gcgttagccc agcgttgata aaaaggatgc tcaggtggca acgacgtcga aacaataatg
    28861 gcatcaacct gacgctgtaa aaggtgctca atgcagcgca tttcgttgtc tggctgatct
    28921 tctgagcagg caatcagcag ttgataaccc cgttgccgcg cctggcgctc aagatagtta
    28981 gcgatgcggg tatagctggt gttctccaga tcggggatca caagaccaat agaacgtgtg
    29041 cgtccagcac gaagcccagc tgccacggcg ttcgggtggt aattgtgctc acgcaccaca
    29101 gccatgactt tttcaacggt tttgtcgctc acacggtatt gcttcgcttt gccgttaata
    29161 acatagcttg cagtggtccg cgacactccc gccagccgag cgatttcatc cagtttcaca
    29221 attgcccctt gcgtaaaatg taaaaaccat agccattgta ctggtatggg ttaaattcat
    29281 taacatctaa gcgcagaaaa ttaactgcgg caactgcttt tattccaggt tcccactgat
    29341 ttcgcaaaaa aagcccagcg tgacatgttg ggctgcaaat tgcacactga gatcattctc
    29401 aacgcattat tttatcgccg cgcgaaagtc cgaccacacc agagcgagca acttccacaa
    29461 ttttcgccac ctcgcgaatc gatgctaaaa atgcatcaag cttatcgctg gtgcctgcta
    29521 attgaacggt ataaagcgag ggggtgacat cgataatttg cccacggaat atttccgtat
    29581 tacgtttcac ttcgtcacgc ccgtaaccgc tggcctgaat tttcaccagc ataatttccc
    29641 gctcaacatg cgcgccctgc cccaactcac tcacgcgcaa gacatcgacc agcttgtgta
    29701 attgcttttc gatctgctca agtacttttt catcgcccac ggtctggatg gtcatacgcg
    29761 ataatgtcgg atcgtcggtt ggcgcaacgg tcaggctttc aatgttgtag cctcgctggg
    29821 aaaaaaggcc aatcacgcgg gataacgcgc ctgattcatt ttcgagtaag actgataata
    29881 tccggcgcat aatcaggttc tctccgtttt gcttaaccac atttcatcca ttccgccccc
    29941 gcgaatctgc atcgggtaga cgtgctcact gccatcgacg gtaacatcaa caaacaccag
    30001 gcgattattg cgcacctgtt ccagcgcctc gctaagtttg ctttccagct catgcggatg
    30061 agagatctgg atcccgacgt gaccataggc ttccgccaga cggacgaaat cgggtagtga
    30121 ttgcatataa gattgtgaat gacggccgga atagatcatg tcctgccact gtttcaccat
    30181 ccccagatag cggttattga gattcaccac cagtacgggc aactcgtatt gcaacgcggt
    30241 agacagttcc tgaatgttca tctgaatact gccgtcgcca gtgacgcaaa ccacggtttc
    30301 ttctggcaac gccattttga cgcccagtgc cgcaggtaaa ccaaaaccca tcgtgccgag
    30361 gccaccggaa ttgatccagc gacgcggttt gtcgaatgga taataaagtg cagcaaacat
    30421 ctggtgctgc ccgacatcgg acgtcacgta agcgtctccc ttcgtcaacc gccaaagagt
    30481 ctcgatcacc gcctgcggtt taatcttttc actgtgagtg tcatatttca ggcactgacg
    30541 agcgcgccac tgttcaattt gctgccacca gtcgcggatc tcatccagtg gttgatgggc
    30601 ggattcttgc gacaagagtt caagcatttg ttcgaggacc tggcgagcat cccccacaat
    30661 cgggatatcc gcagtcacgg ttttagaaat ggaagtagga tcaatatcga tatgcagaac
    30721 agtggcattt gggcagtact ttgccagatt gttcgtcgtt cggtcatcaa atcgtacccc
    30781 gacggcgaaa atcacatccg cgttatgcat cgtcatattg gcttcgtagg taccgtgcat
    30841 tcccagcatg cccagtgcct gacgatgcgt tgccggaaac gcccccagcc ccatcaatga
    30901 ggaaacaacg ggcagattca acgcctccac cgtttctttc aactgctgat ggcagcccgc
    30961 catgattgcc ccaccgccta cgtagacaac cggttttttt gccactacca gcgtttgcag
    31021 agcacgctta atttgccctt tatgtccggt agtagtggga ttgtaagaac gcatactgac
    31081 cgactccggc cagacatagg gtaatttgtt cgccggatta agaatatctt tcggtaaatc
    31141 aacgactact ggtccaggac gaccacttgc cgccagccag aaagcctttt tcagcacctg
    31201 cggaatgtct tccgtttgct taaccagaaa actgtgttta accactggtc gcgaaatccc
    31261 caccatgtcg cactcctgaa aggcatcgta acctatcaac gaggtcgcta cctgcccgga
    31321 aaggacaact aatggaatgg aatccatata agcggtggcg atgccggtaa tcgcattggt
    31381 cgcccctgga cccgacgtta ccagcacgac gccgacttcc ccggtcgcgc gcgccagacc
    31441 atcggccata tgcactgccg cctgctcatg acgaactaaa acatgatcga taccacccac
    31501 ggtatgcaat gcatcgtaaa tatcaaggac tgcgcctcca ggataaccga atacttgttt
    31561 aacgccctga tcgataagcg atcggacgac catctcggct ccagacaaca tctccatggc
    31621 ctgcctcact gtttgatgga aaaaatgtgt aaaacacatt aaccgtccga tcttgcttaa
    31681 gcaattggca gccagagaca taaacgcagg gtaataagaa taaacaggag gaaagatgaa
    31741 aagaaacgat aaaatcctcc attacgaagg attaaattga taataaatcg gctgaatccc
    31801 acaacttaca gcaatttctc tattttttta attaatccta aaatagagag cccacacatt
    31861 cagaataaaa ttctacattc aataaaaaat gcagaataaa ccagacattc atgtctgacc
    31921 tattctgcat tcagttagcg tttgcaaatt gagactaatt gttcttccat ccactgatgg
    31981 cctttatcgc gcccggcagc ttcatgccat gagagataac aggttctgct gttttgtttt
    32041 aacggcagcg gtaatacctg taattctaag gattcagcga actcttcagc cagccaacgc
    32101 ggcgcaatag cgaccaaatg cgtttgcgac accacgctaa gtacgctcat cattgccatg
    32161 ccctgatacg cgatactggc ttgcttatct accgtgtcat accaaggttg actaaatgac
    32221 gcgaaacgat cgagcgaaac cgccgcatgt tgttcgttat aaacatcatg tttcagtaac
    32281 gggcctttaa ttgttggatg atttttgctg gctaccagca ccatttcatc tttaaataat
    32341 ggcacgctgg taaattcagg acgatggaag tcttcataac taatcacaaa ctccgtttcc
    32401 tgataacgca gctgatgttc agtgttctga tttaatgaag acttgaacat aacatgtata
    32461 tttggcgcaa tctgctcaat gtgattataa atctgcgagg tcagaatgct gtctaacggg
    32521 ctgcaaacac aaagatgaaa tacacgttca ctactcgcgg gttcaaaacc tgaaccaggc
    32581 aattcatttt gtactagttg caatgcctgg cgaactgaac caaaaagttg aaatgcgcga
    32641 gcagtcggtt gaataccacg gccataacga acaaaaagct cgtcattaaa catcaccttc
    32701 aggcgtgcaa cagcgttact gaccgcaggt tgcgacatac ccagaacatg agcggcacgg
    32761 gtaatgtttt gctcctgcat cacggcatcg aaaacggtta ataagttgag atcgaccatg
    32821 cgtagctgtg gcttgcttaa ctccgccgtc tctgaatgat ctgtttgtac ctctggcata
    32881 cttaactcca ctgtcacact taactccctt tcccttattg gaatgcagag aaataatcct
    32941 gaagaatatg atttatcatg caggtaatga ctccaactta ctgatagtgt tttatgttca
    33001 gataatgccc gatgaccttg tcatgcagct c
//
LOCUS       DAJVUJ010000027        32490 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.027, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000027 DAJVUJ010000000
VERSION     DAJVUJ010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32490
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.027"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            198..992
                     /gene="panB"
                     /locus_tag="OQQ48_001193"
     CDS             198..992
                     /gene="panB"
                     /locus_tag="OQQ48_001193"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HCS1952038.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTASVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1004..1855
                     /gene="panC"
                     /locus_tag="OQQ48_001194"
     CDS             1004..1855
                     /gene="panC"
                     /locus_tag="OQQ48_001194"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HCS1952039.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIIAIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKIVELA"
     gene            complement(2147..3043)
                     /gene="rpnC"
                     /locus_tag="OQQ48_001195"
     CDS             complement(2147..3043)
                     /gene="rpnC"
                     /locus_tag="OQQ48_001195"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HCS1952040.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQGKPDKKMAFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDIFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGIEKGIQQGRQEERQEFAQRFLSKG
                     MSREDVAEMTNLSLAEIDRLIN"
     gene            3317..3697
                     /gene="panD"
                     /locus_tag="OQQ48_001196"
     CDS             3317..3697
                     /gene="panD"
                     /locus_tag="OQQ48_001196"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HCS1952041.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(3701..4901)
                     /locus_tag="OQQ48_001197"
                     /pseudo
     CDS             complement(3701..4901)
                     /locus_tag="OQQ48_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001727225.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
     gene            complement(4996..5436)
                     /locus_tag="OQQ48_001198"
     CDS             complement(4996..5436)
                     /locus_tag="OQQ48_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCS1952042.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRYEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGAPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5541..6311)
                     /locus_tag="OQQ48_001199"
     CDS             complement(5541..6311)
                     /locus_tag="OQQ48_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1952043.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLATTFGVLVVFIVVFYLICWSLIQRGRGLRS"
     gene            complement(6308..7234)
                     /locus_tag="OQQ48_001200"
     CDS             complement(6308..7234)
                     /locus_tag="OQQ48_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS1952044.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKLETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7343..8005
                     /gene="can"
                     /locus_tag="OQQ48_001201"
     CDS             7343..8005
                     /gene="can"
                     /locus_tag="OQQ48_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HCS1952045.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPHFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8046..8582)
                     /gene="hpt"
                     /locus_tag="OQQ48_001202"
     CDS             complement(8046..8582)
                     /gene="hpt"
                     /locus_tag="OQQ48_001202"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCS1952046.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            8788..11178
                     /gene="gcd"
                     /locus_tag="OQQ48_001203"
     CDS             8788..11178
                     /gene="gcd"
                     /locus_tag="OQQ48_001203"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HCS1952047.1"
                     /translation="MAINNTGSRRLLVTLTVLFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGSILTWAGFNDPQEINGTLSADATPTEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPKLKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPKKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11225..12775)
                     /gene="cueO"
                     /locus_tag="OQQ48_001204"
     CDS             complement(11225..12775)
                     /gene="cueO"
                     /locus_tag="OQQ48_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HCS1952048.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHDAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            12941..13288
                     /locus_tag="OQQ48_001205"
     CDS             12941..13288
                     /locus_tag="OQQ48_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HCS1952049.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13393..14259
                     /gene="speE"
                     /locus_tag="OQQ48_001206"
     CDS             13393..14259
                     /gene="speE"
                     /locus_tag="OQQ48_001206"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HCS1952050.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENTAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPSGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14275..15069
                     /gene="speD"
                     /locus_tag="OQQ48_001207"
     CDS             14275..15069
                     /gene="speD"
                     /locus_tag="OQQ48_001207"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HCS1952051.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15106..15468)
                     /locus_tag="OQQ48_001208"
     CDS             complement(15106..15468)
                     /locus_tag="OQQ48_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HCS1952052.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(15643..18240)
                     /gene="acnB"
                     /locus_tag="OQQ48_001209"
     CDS             complement(15643..18240)
                     /gene="acnB"
                     /locus_tag="OQQ48_001209"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HCS1952053.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18595..20448
                     /locus_tag="OQQ48_001210"
     CDS             18595..20448
                     /locus_tag="OQQ48_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HCS1952054.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAAVETQSTQ
                     LAVSDAAAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDALLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSNEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTASPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FIDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVRERMQTNPQIQQRREAARERIQPASPEQRQ
                     AVREKMQTNPQIQQRRDAARERIQSASPEQRQVFKEKVQQRPLNQQQRDNARQRVQSA
                     SPEQRQVFRERVQESRPQRLNDSNRTARLNNDQRSAVRERLSERGARRLER"
     gene            complement(20519..21943)
                     /gene="lpdA"
                     /locus_tag="OQQ48_001211"
     CDS             complement(20519..21943)
                     /gene="lpdA"
                     /locus_tag="OQQ48_001211"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCS1952055.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22268..24160)
                     /gene="aceF"
                     /locus_tag="OQQ48_001212"
     CDS             complement(22268..24160)
                     /gene="aceF"
                     /locus_tag="OQQ48_001212"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HCS1952056.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(24175..26838)
                     /gene="aceE"
                     /locus_tag="OQQ48_001213"
     CDS             complement(24175..26838)
                     /gene="aceE"
                     /locus_tag="OQQ48_001213"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HCS1952057.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHSI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(26999..27763)
                     /gene="pdhR"
                     /locus_tag="OQQ48_001214"
     CDS             complement(26999..27763)
                     /gene="pdhR"
                     /locus_tag="OQQ48_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HCS1952058.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28304..29677
                     /gene="aroP"
                     /locus_tag="OQQ48_001215"
     CDS             28304..29677
                     /gene="aroP"
                     /locus_tag="OQQ48_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HCS1952059.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNSGPQATVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(29720..30574)
                     /gene="ampE"
                     /locus_tag="OQQ48_001216"
     CDS             complement(29720..30574)
                     /gene="ampE"
                     /locus_tag="OQQ48_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HCS1952060.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARVTMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGIWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30571..31122)
                     /gene="ampD"
                     /locus_tag="OQQ48_001217"
     CDS             complement(30571..31122)
                     /gene="ampD"
                     /locus_tag="OQQ48_001217"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HCS1952061.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31210..32103
                     /gene="nadC"
                     /locus_tag="OQQ48_001218"
     CDS             31210..32103
                     /gene="nadC"
                     /locus_tag="OQQ48_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HCS1952062.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32306..>32490
                     /locus_tag="OQQ48_001219"
     CDS             32306..>32490
                     /locus_tag="OQQ48_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCS1952063.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGG"
BASE COUNT         7833 a         8833 c         8006 g         7818 t
ORIGIN      
        1 ggccacgcta caacaggatg gaactatacc aatagaagct ggtgagttta aagccaccag
       61 tactttccag gtaacctacc cttaataagt ccaccgcacc gccatcatct ggcggtgcgg
      121 taattgataa atcattgccc ccaccaatgt gactgataaa atgccaggca acacgacatc
      181 atttatcagg acacgttatg aaaccgacca ccattgcttc acttcagaaa tgtaagcagg
      241 ataaaaagcg tttcgcgacc atcaccgctt acgactacag cttcgcgaaa ctatttgctg
      301 atgaaggact taacgtcatg ctggtaggcg actcgctggg catgacggtt cagggtcacg
      361 actccaccct gccggtgacc gttgccgata tcgcctacca cactgcctcc gtacgtcgtg
      421 gtgcgccaaa ctgcctgctg ttggctgacc tgccgtttat ggcgtatgcc acgccggaac
      481 aagcctttga aaacgccgca acggttatgc gtgccggtgc caatatggtc aaaattgagg
      541 gcggtgagtg gctggtcgaa accgtaaaaa tgctgaccga acgtgccgtt cctgtatgtg
      601 gtcacttagg tttaacccca cagtcagtga atattttcgg tggctacaaa gttcaggggc
      661 gcggcgatga agcgggcgat caactgctca gcgatgcatt agccttagaa gccgctgggg
      721 cacagctgct ggtgctggaa tgcgtgccgg ttgaactggc aaaacgtatt accgaagcac
      781 tggcgatccc ggttattggc attggcgcag gcaacgtcac tgacgggcag atcctcgtga
      841 tgcacgacgc cttcggcatt accggcggtc acattcctaa attcgctaaa aatttcctcg
      901 ccgaaacggg cgacatccgc gcggctgtgc ggcagtatat ggctgaagtg gagtccggcg
      961 tttatccggg cgaagaacac agtttccatt aaggagtcac gttgtgttaa ttatcgaaac
     1021 cctgccgctg ctgcgtcagc aaattcgccg cctgcgtatg gaaggcaagc gcgtggcgct
     1081 ggtgcctact atgggtaatc tgcacgatgg ccatatgaag ctggtcgacg aagccaaagc
     1141 ccgcgccgat gtggtcgtcg tcagtatttt cgttaacccg atgcagttcg accgcccgga
     1201 agatctggca cgttatccac ggaccttgca ggaggactgc gagaagctaa acaaacgtaa
     1261 agtggattta gttttcgccc cttcggtaaa agagatctac ccgaacggta ctgaaaccca
     1321 tacttacgtt gacgttcccg ggctttcgac catgctggaa ggcgcgagcc gtccggggca
     1381 ttttcgcggc gtttcgacta tcgtcagcaa gctgtttaac ctggtccagc cggacatcgc
     1441 ctgcttcggt gaaaaggact ttcagcaact ggcgctaatc cgcaaaatgg ttgccgatat
     1501 gggcttcgat attgaaattg tcggcgtccc gattatgcgc gccaaagatg gccttgcgct
     1561 cagttcccgt aatggttatc tgacggcgga acaacgcaaa attgcgcccg gtctgtacaa
     1621 agttttaagt tcaattgccg acaaattgca ggctggcgag cgggatcttg atgaaattat
     1681 tgccattgca gggcaagaac tgaatgaaaa aggcttccgc gccgatgata ttcagattcg
     1741 cgatgccgac acgttgctgg aagtctctga aaccagcaaa cgagctgtaa ttctggtagc
     1801 cgcctggctt ggcgatgccc gcctgatcga caacaaaatc gtcgaactgg cgtaatgctt
     1861 ttactctaaa taattcaagt tgcaggaagg cggcaagcga gtgacaaatt cgtcaggaac
     1921 gaatttgccc agccgcaggc tggccttcgg tgagagacat ggatgtctct cattaatccc
     1981 caggagctta cataagtaag tgactggggt tagcgaacgc agacacagca catgcaattt
     2041 aaagtatgac gagtataact ggcgctacgg ctgatggcgc caattattaa tttgccccac
     2101 gtcatggcga tggcgtctgt aagcaattat tcataacgca gacaacttag ttaatcagcc
     2161 tatcaatctc agcaagagat aaatttgtca tctctgcaac gtcttcccga gacatccctt
     2221 tactcagaaa acgctgggcg aattcttgcc tttcctcctg tcttccctgc tgaatcccct
     2281 tctcaatccc tttctcttca aaccactgcg ccagcgtcat catagactcc cctcccgttt
     2341 ccctgtctct cagcacacca taaaacaaat ccgcttgttc agtatgaccg cgttgcagca
     2401 tatagttttg catggcaact aactgacttc cgctagtgta cccttcgtca atcagcgtga
     2461 ccagttgctc cagcaataac attaagtcgc gctggcgaat atgtttttgc agtagttcga
     2521 gaatcgcaat ccgccgatgt tgcatgattt cgtcatccgg tgtgatggtg atatccacca
     2581 gcgggaaagg actgttatag acgcgtcgtg ccagctccgg cgagtaaaat atatcaaacc
     2641 agcacattga gagcggataa ggtgtggcct cgccctgata aaacagtatc ggcaccacca
     2701 gcggcagctt atcgtgatcc gcctccagat gccggtgcat ggcagcaata gaataacgca
     2761 tcatgcgaaa ggccattttc ttatccggct tgccttggtg ttcaatcaca acatgcagat
     2821 aaccgggatt gccctgcatt tgcacggaat agagcacgtc cgtgctgtgt cctttcaggc
     2881 tctcttcaat gaaactcccc gactctaaat gaagcgtgtt gaggtcacaa agttctcgta
     2941 attccactgg caaatgtatc tccagaaagt cgcgagccgt ctccgcgtgc attaaaaatt
     3001 gtttaaacac tgcatcatgc ggtgtggtac tcggtgcatc catcttccct gccttccgtg
     3061 ttgtcttgcg tgaaggcagt atccagtcaa ggcgtgccat cgtcagccac ctctttagtg
     3121 tttaccgagc agcgttcaga gaaatttcaa ttggttacag ccgttacatg gcacgcttcg
     3181 gcacggtacg caaactgctg atgtaaatgc cgtcagatga gtagacacta aacaaaaatc
     3241 gggcaatact gcgtgagaat ttcctcaaag caggcatcgc ctgcttcgtt aacgacaggg
     3301 tagaaaggta gaagttatga ttcgcacgat gctgcagggc aaactccacc gcgtgaaagt
     3361 gactcatgcg gacctgcact atgaaggttc ttgcgccatt gaccaggatt ttcttgacgc
     3421 agccggtatt ctcgaaaacg aagccattga tatctggaat gtcaccaacg gcaagcgttt
     3481 ctccacttat gccatcgcgg cagaacgcgg ttcgagaatt atttctgtta acggtgcggc
     3541 ggcccactgc gccagtgtcg gcgatattgt catcatcgcc agcttcgtta ccatgccaga
     3601 tgaagaagct cgcacctggc gacccaacgt cgcctatttt gaaggcgaca atgaaatgaa
     3661 acgtaccgcg aaagcgattc cggtacaggt tgcttgattg ttatccctgc ggctggttac
     3721 tcaccagccg cgacatcgtc tccagcgaat ccgttcttaa gatataaagt cgttttagta
     3781 acaacggatt atcccccggt tttactttgc ctttcatggt tgtcaccgcc aggtgaaatc
     3841 cggcatcgtt tgctgccttc acggctttgt cattaaatcc gccaaacgga tacgaaagat
     3901 accagacatg cggattaaat tgcgccagag cgcggcgtga acgtgcaaaa tcaaacagaa
     3961 tattgtgttc actacggctc ggtaatatgg ggcggcgata accatccacc cgatgcaaaa
     4021 aatgggtatg tgactggaaa tcaaaaacat cgcgaatttc gttaagttca gaaacgctca
     4081 taaattgcag cgattttggg tcccacttct gcgggtgacg tttgatgcgc gaggtaacaa
     4141 taaacgccgt cgccttcatg ccatattgtt tcaacacggg atacgcatag cggctcaccg
     4201 atttgaggcc atcatcaaag gtaatcacca ccgctcgcgc agggagattg atcttattct
     4261 tcacgtaacc ttccagctgc accatgctca gtgtcgcgta tcccctgtcg cgcagccagg
     4321 ccatctggtt attgaaagcg cgtactgagg tggtcgtcga agtatggcga aaacgggtgt
     4381 tttcttcatc gcgcagaata tggtgatagg ttagcaccgg caggccatta tcgggttggg
     4441 catccagcgc gctgatatag gccagtcgat cgccaatacg gatctgatac caggtttgat
     4501 ttaacctgtc tttcagttta tgcaaaatcg ggtagcgcaa attgtccgcc agtaccccat
     4561 aatggcgcac tgcccgcact cggcgcgtta tagaccggcg tatctttcca ggtaattaag
     4621 ttctgattac tcagcggctt gttgaggtca cccaaaccgt cttcaacttt ttgtcgcccc
     4681 tgaactggct cgagatgccc tttatcgata aaccctttac caaagccaaa attaaatgcg
     4741 taataacttg cggcagtggg ctccacggca atgatttgcc cggcccgaat attccccacg
     4801 gtcaccatct tgtcgccaat ttgcgcccag atcgcagcat tttcgatggt ttgcatataa
     4861 cgggcaggta acgcggcact aacgcttgcg gtaaacagca tcagcaggat gaataacagc
     4921 ttgtttatac atgaaaaaat aaccgagggt gaaacaacgc ttgtattgta gcaaaagcgc
     4981 catcagaatc gcgggttaat actcatacaa attatgtttg aggacaaaag gcggattttt
     5041 ttgttgttgg tgccaaagac tactcacctc cggcgcaccc agttcgacaa tacgctcgcg
     5101 aaactctgaa ctggtcatgg tttcacggca agccatcatc tgttcaatta acggtaacgt
     5161 gacaccagaa atcacttcat aacgggagtg tttatggctt aataatgaag ccacgcggta
     5221 cggtggcgct ccggctatat cggttaagaa gatgacaccc tcaccagagt ccgcttcatg
     5281 cagagcatcg cacatcatgc ggctgagcat attagagctt aatccgcgcc agaaattcac
     5341 ggcccggcac tgaagaagtg ccccatgttt tttctccagc gcatccagta tctcttgcgc
     5401 ccggtcatcg tgacaggtaa ttacccaacc taacatcgcc ttcctcctta gccgaaaatc
     5461 agtctatcct gaccattgtt gataaagggt gatagccgtc aaaactcctc tcccccgaat
     5521 ccagaggaga ggaaatagcc ttagctacgc aaaccacgtc cacgttggat cagcgaccaa
     5581 cagatcaaat aaaacaccac aataaagacc accagtacgc caaaggtagt ggccagcggc
     5641 acatcattta taccgaggaa gccgtagcgg aaaccactga tcatataaac gattgggttc
     5701 aggtgcgaca gcccttgcca gaacggcggc aacaaagtca gggagtaaaa caccccgccc
     5761 aaatacgtga gtggcgttaa cacaaaggtt ggcaccaggc tgatgtcatc gaacgttttg
     5821 gcaaacacgc cgttcagcaa acccgcaagg gagaatagca ccgccgtgag caccagggtt
     5881 aaggcaacga atacccacga atgcacctga aacggcacaa aaaacagtga aattgccgtc
     5941 accagaatgc caacaaacag accacgcgcc acgccaccgc cgacatatcc ggcaataatg
     6001 acgtgagtcg gaaccggcgc taccagcagc tcttcaatat tacgctggaa cttggcacca
     6061 aaaaatgatg acgcgacgtt agcgtaggca ttggtgatca ccgacatcat gatcagcccc
     6121 ggtacgatga actgcatata gctgaagcca tgcatatcgc caatacgcga acctatcagg
     6181 ttaccgaaga taataaagta aagggtcatg gtgatgactg gcggcaccag cgtctgcacc
     6241 cagatacgca taaagcgatg gatctctttc gcccagatgc tttttagcgc cacccagtaa
     6301 agatgcatca tgcgcgatct ccttgttttt cattaaccag tgaaacaaac agctcttcca
     6361 gacggttagc tttgttacgc atacttaata cctgaatgcc ctgctcactt aactgcgtaa
     6421 atacgctgtt gatcccctgc tcacgcagca cttcaacttc cagcgtcgcg gtatcgacca
     6481 gtcgatactg atagccatcg agcttcggta acgggctttt cggtgcgaga tcgagaataa
     6541 aggtttccaa tttcagcttc gccagcagcg ccttcatcga ggtattttcc accagctcac
     6601 cgtgttgaat aatgccgata ttgcggcaca gcatttctgc ttcttccagg tagtgtgtag
     6661 tgagaatgat ggtggtgcct ttgtcgttta aatccttcaa aaagccccac attgagcggc
     6721 gaagttcaat atccacgcct gcggtcggtt cgtcgagaat cagtagttta ggttcatgca
     6781 ttaacgcacg ggcaatcatt aaacggcgct tcatcccgcc agataacata cgcgcacgtt
     6841 cgttgcgttt tccccataaa tcgagttgtt tgagatattt ttcgctgcgg atgtacgctt
     6901 ctttgcgctc cacgccgtag taccctgcct gattcaccac aatttgctgc acggtttcaa
     6961 acgggttgaa gttaaattcc tgcggcacca gtcccaactg acgtttagcg ttcacgacat
     7021 ccttctcgag atcgtaacca aatacgctga cccgcccgga ggttttattt accagagagc
     7081 tgataatacc gatagtggtc gatttcccgg ccccgttcgg cccgagaagc gcataaaaat
     7141 cacccgcttc gacctgcaaa tctattccac gaagcgcctg aacgccgcct ggataggttt
     7201 ttttaagctg ttgaagttcc agtgcaatgg tcataaattt ttacttacct tacgttctta
     7261 cactttatct gtggtttaaa tcgtcccgga gttaccctat attagccaaa cgtaattatt
     7321 tggttacagg tcgttaacct ccatgaaaga catagataca ctcatcagca acaatgcact
     7381 atggtcaaaa atgctggtgg aagaggatcc cgggtttttt gagaaactgg cacaagcgca
     7441 aaaaccgcac tttctatgga ttggatgttc cgacagtcgc gttcctgcag aacgtttaac
     7501 cggtcttgag ccgggcgaac tctttgttca ccgtaatgtt gctaacctgg tcattcacac
     7561 cgacctgaac tgcctttccg tggttcagta tgcagtggat gtactagaag ttgaacacat
     7621 tattatctgt ggccactacg gttgcggcgg cgtacaagcc gcagttgaaa acccggaact
     7681 ggggcttatc aacaactggc tgctgcatat ccgcgatatc tggttcaaac atagctcatt
     7741 gctcggcgaa atgccgcaag agcgccgtct ggataccttg tgtgaactga acgtcatgga
     7801 acaggtgtat aacctaggcc actccaccat tatgcaatca gcgtggaaac gcgggcagaa
     7861 agttaccatt cacggctggg cctatggcat tcacgacggc ttgctgcgtg atctggatgt
     7921 tactgccacc aaccgcgaaa cccttgagca acgttaccgt cacgggattt ccaacctcaa
     7981 gctgaaacac gccaaccaca aataaaaatg ccatgccgga tgcaacacat ccggcaactt
     8041 cacacttact cgtccagcag aatcactttg ccgatatacg gcagatgacg gtaacgctgt
     8101 gcgtaatcaa tgccgtaacc caccacaaac tcatccggga tcgagaaacc gataaattct
     8161 accgggacgt tcacttcacg acgggacggt ttatccagca gcgtacaaat cgccagcgac
     8221 ttcggttcgc gcaggcttaa gatctcacgc actttcgaca gtgtattccc cgagtcgatg
     8281 atatcttcaa caatcagcac gtccttgcca cggatatctt catccagatc tttgaggatt
     8341 ttcacatcac gggtggtgga catgccgcta ccgtagctgg aggcggtcat aaagtcgact
     8401 tcatgagata cctgaacttc acggcacagg tccgccataa acataaatga gccacgcagc
     8461 agacccacca gcaccatatc gctgccgctg tctttgtaac gctcagtaat ctgacgaccc
     8521 agttcggcga tacgcgcttt aatctccgct tcggggatca ttacttctac agtatgtttc
     8581 atatctctaa ccatatgatt taaaaacaaa tcacttcgca cgggcaggtt ttggcaccgc
     8641 aacaaagcgc aagacacgca gtataccagt aaaactattc attcgcggaa tctgttaata
     8701 aagctcgaat tgtgatgacg atcacacatg ttaaaaccta taattaattg ctactaaaat
     8761 attaatgaat tgaaatggtg tctctttatg gcaattaaca atacaggctc gcgacgatta
     8821 ctcgtcacgc taacagtcct ttttgcagcg ctttgcgggc tgtatctact cattggcgga
     8881 ggctggctgg tcgcgattgg cggctcctgg tactacccta tcgctggcct tgtgatgctc
     8941 ggcgtcgcct ggatgctgtg gcgcagtaaa cgtgccgcgc tttggctata cgccgccctg
     9001 ctgctcggca ccatgatttg gggcgtctgg gaagttggtt tcgacttctg ggcgctgact
     9061 ccgcgcagcg atattctggt cttcttcggc atctggctga tcctgccgtt tgtctggcgt
     9121 cgcctggtca ttcctgccag cggcgcagtt gccgcactgg tggtggcact gctgattagc
     9181 ggtagtatcc tgacctgggc cggatttaac gatccgcagg aaatcaacgg caccttaagc
     9241 gccgatgcca cacctacgga agctatctca cccgttgccg atcaggactg gcctgcctat
     9301 ggtcgcaatc aggaaggtca acgcttttca cctctgaaac aaattaacgc cgataacgtc
     9361 cataatctga aagaagcctg ggtgttccgt actggcgatg tgaagcagcc gaacgatccg
     9421 ggcgaaatca ccaatgaagt gacgccgatt aaagtgggcg acacccttta cctgtgtacc
     9481 gctcaccagc gcctgtttgc gctcgatgcc gccagcggca aagagaaatg gcattacgat
     9541 cctaagctga aaactaacga gtctttccag cacgtaacct gccgtggcgt ctcttatcat
     9601 gaagccaaag cagaaacggc ttcgccggaa gtgatggcgg attgcccgcg tcgtatcatt
     9661 cttccggtca atgatggtcg actgattgcg attaacgctg aaaacggcaa actgtgcgaa
     9721 accttcgcca ataaaggcgt gctcaatctg caaagcaata tgccagacac caaaccgggt
     9781 ctgtatgaac cgacttcgcc accgattatc accgataaaa ccatcgtgat ggcaggttca
     9841 gttaccgata acttctcaac ccgcgaaacg tctggcgtga tccgtggttt tgatgtcaac
     9901 accggggagc tgctgtgggc ttttgatccc ggcgcgaaag atccgaacgc aatcccgtct
     9961 gacgaacaca cctttacctt taactcgcca aactcctggg caccagcggc ctatgacgcg
    10021 aagctggatc tggtctatct gccgatgggc gtgaccacgc cggatatctg gggcggtaac
    10081 cgcacaccgg aacaggaacg ttatgccagc tcgattctgg cgctgaatgc cactaccggg
    10141 aaactggcgt ggagctacca gaccgttcac cacgacctgt gggacatgga tcttccggca
    10201 cagccgacgc tggcggacat caccgttaat ggtcagaaag tgccagttat ttacgctccg
    10261 gcgaaaaccg gcaacatttt tgtgctcgat cgtcgcaatg gcgaactggt ggttccggcc
    10321 ccggaaaaac cggtgccgca aggtgctgcg aaaggcgatt acgttactcc gacccagccg
    10381 ttctctgaac tgagcttccg tccgaagaaa gatttgagcg gtgcggatat gtggggagcc
    10441 accatgtttg accaactggt gtgccgcgtg atgttccacc agatgcgcta tgaaggcatt
    10501 ttcacgccgc catctgaaca gggcacgctg gtcttcccgg gtaacctggg aatgtttgaa
    10561 tggggcggga tttccgttga tccaaatcgt gaagtggcaa ttgccaaccc aatggcactg
    10621 ccgtttgttt cgaaactgat cccacgcggt ccgggtaacc cgatggagca gccgaaagat
    10681 gccaaaggca cgggtacgga atccggcatt cagccacagt acggtgtacc gtatggtgtc
    10741 acgctcaacc cgttcctctc accgtttggt ctgccgtgta aacagccagc atggggttat
    10801 atctcggcgc tggatctgaa aaccaatgaa gtggtgtgga agaaacgtat tggtacgccg
    10861 caggacagta tgccgttccc gatgcctgta ccggtgccgt tcaatatggg tatgccgatg
    10921 ctgggcgggc caatctccac ggcgggtaac gtgctgttta tcgccgctac ggcagataac
    10981 tacctgcgcg cttacaacat gagcaacggt gaaaaactgt ggcagggtcg tttaccagcg
    11041 ggtggtcagg cgacgccaat gacctatgaa gtgaatggta agcagtatgt ggtgatctcc
    11101 gcaggcggtc acggttcatt tggtacgaag atgggcgact atattgtggc ttatgccctg
    11161 ccggatgatg tgaaataacc ataatgcccg gagagatccg ggcatatttc cgaatacggt
    11221 ctttttatac cgtaaaccct aacatcatcc ccgtatcttc atgctccagc agatggcagt
    11281 gcgccatata agcatgttct ttcggtgcgt cgtgattaaa cttcaccagc acttcactga
    11341 cattgccttc taccttaacg gtatctttcc agcccgcgcg atgagccgct ggcggtttgc
    11401 cattttctga caagatacgg aactgcgtgc cgtgaatatg gaacggatgc agcatcatgt
    11461 cgcccacgcc agagataacc caacgttcgt actgcccttt cgccgccgca aacatcggct
    11521 tattcatatc aaacgcctga ccgttgattt tattggcatg gtggaaatcg aacttcccgc
    11581 cgtggttcat atgattcata ttaccgtgcc ccatatggcc catcatctgg ctgtgatcca
    11641 ttccggccat cgcctgatcg ccatatttct ccattagcat ctgcataccc atcatatcga
    11701 gcatcgggtc catagaaagt tgcagcttgc gtaccgtcag cccttccagc gaaggtaacg
    11761 caggcaggct acttaatgtg tctggcaaag caccggaggc actaatagca atcggctgaa
    11821 tccgcattac cggatgcggc ttgtcaaacg gcgcaatcgc catccccatc tggctgaccg
    11881 gtagcgtcac caggtcaaag ggtttgttgt cgttaacctc caccagcact tcaaaacgct
    11941 cgcccatcag caccggcagc tcgctcacct tcaccggttc aggtagcaga ccaccgtcgc
    12001 tggcaatcac atacagcggg cgattgtcgc tggtggcgaa attgagcgag cgggcattac
    12061 agccattgag caaacgcagg cgcagccaac cacgcggggc agcgtgttgt gggtagattg
    12121 caccgttggt cagcagcgta tcgccaaacc agcccacggc ggcggtcatc acatccagtt
    12181 gataatcaat ctgcccatca gcgttaaatt ttttatcctg aacaatcacc ggaacatcat
    12241 cgatacccca ctgttttggc agcattaatt tcaggatctc gtcatcttca attaccacca
    12301 gcccagccaa ccccatcgcc acctgtcgcc cagtcttgcc atgttgatgt ggatggaacc
    12361 agcaagtagc ggcaggttga tcaacgttca acgtcaccga gcgcttgcca cctggcggaa
    12421 taattccctg cgggccgccg tcgacttcac ccggtacttc cagcccgtgc cagtgcaacg
    12481 tcgtctcttc cgtcagttgg ttatagatat caaccgttac cgctttgccg cgctgtaatt
    12541 tcaccgccgg ccccagcaga ttgccgttat agccccaggt agttgcagtt ttctcgccaa
    12601 aggtagactg acctgcgcca atagttaact gaatgcgatt acgggcatcg gtcgtgagca
    12661 aatcagggat cggtaacgtt gggcgttccg ccgcaaagac tgcgcggctc cacagcggca
    12721 aggctgaagc cacacccagc gcgacggaat attttaagaa atcacgacgt tgcatagtta
    12781 tttccttatt cttaagcagg cgaaacaaaa tcaaacgttg agcatagtcc ttccccttac
    12841 gggaaggtca agccgcagac acaatttaat cgacaataat ggtcgtcgcc tcgctcgcag
    12901 tgtgctaacg tttatcttct ttaagcccct ggtagaagca atgaagacgt ttttcagaac
    12961 agtgttattc ggcagcctga tggccgtttg cgcaaacagt tacgcgctca gcgagtctga
    13021 agccgaagat atggccgatt taacggcagt ttttgtcttt ctgaagaacg attgtggtta
    13081 ccagaactta cctaacgggc aaattcgtcg cgcactggtc tttttcgccc agcaaaacca
    13141 gtgggatctc agtaattacg acaccttcga catgaaagcc ctcggtgaag acagctaccg
    13201 cgatctcagc ggcattggca ttcccgtcgc taaaaagtgc aaagccttgg cccgcgattc
    13261 cttaagcctg cttgcctacg tcaaataatc cctgatacct ttttgtagaa atattgaccg
    13321 tgcatccgcg gtcaatgtta gctattatgt tgcgcccttt tttacgggtg ttaacaaagg
    13381 aggtatcaac ccatggccga aaaaaaacag tggcatgaaa cgctacacga ccagtttggg
    13441 cagtactttg cggtagataa cgttctgtat catgaaaaga ccgatcacca agatctgatc
    13501 atttttgaga acactgcatt tggtcgcgta atggcgctgg atggcgtagt acaaaccacc
    13561 gagcgcgacg agtttatcta tcatgagatg atgacccatg ttccgctact ggcccacggt
    13621 cacgcgaaac atgtgctgat tatcggcggc ggcgacggtg ccatgctgcg tgaagtaacc
    13681 cgacataaaa acgttgagtc aatcacgatg gtagaaatcg atgcgggtgt cgtgtcgttc
    13741 tgccgtcagt atctgcccaa ccataacgcc ggtagctacg acgatccgcg ctttaagctg
    13801 gtgatcgacg atggcgtcaa tttcgttaat caaaccagcc agacctttga tgtcattatc
    13861 tccgactgca ccgatcctat cggtcccggc gaaagtctgt tcacctcagc attttatgaa
    13921 ggctgcaaac gttgcctgaa tcctagcggt atcttcgtcg cacaaaacgg cgtctgcttt
    13981 ttacagcagg aagaagccat cgacagccat cgcaaactca gccattactt cagcgacgtt
    14041 ggcttttatc aggcggcgat cccgacctat tacggcggta tcatgacttt tgcatgggcg
    14101 acagataacg acgccttacg ccatctctca accgaaatta ttcaggcgcg ttttctcgct
    14161 tctggcctga aatgccgtta ttacaatccg gcaatccata cggcagcttt tgccttacct
    14221 cagtatctgc aagatgcact ggcttcacag ccgtcctaag gagaagataa gaaattgaaa
    14281 aaactgaaac tgcatggctt taataatctg accaaaagtc tgagtttttg tatttacgat
    14341 atctgctacg ccaaaactgc cgaagagcgc gacggttata ttgcttatat cgatgaactc
    14401 tataatgcca accgtctgac cgaaatcctg tcagaaacct gttccattat cggggccaat
    14461 attcttaaca tcgcccgcca ggattacgaa ccacagggtg ccagcgtcac tattctggtg
    14521 agtgaagaac cggttgaccc gaaactcatc gacaaaacag aacaccccgg cccactgcca
    14581 gaaacggtcg ttgcccatct cgataaaagt catatttgcg tacataccta cccggaaagc
    14641 catcctgaag gtggtttatg taccttccgc gccgatattg aagtctctac ctgcggcgtg
    14701 atttctccgc tgaaggcgct gaattacctg atccaccagc ttgagtccga tatcgtaacc
    14761 attgattatc gcgtgcgcgg ttttacccgc gacattaacg gtatgaagca ctttatcgac
    14821 catgagatta attcgattca gaactttatg tctgacgata tgaaggcgct gtatgacatg
    14881 gtggatgtaa acgtctatca ggaaaatatc ttccatacca agatgttgct taaagagttc
    14941 gaccttaagc actacatgtt ccacaccaaa ccggaagatt taaccgacag cgagcgccag
    15001 gaaattaccg ctgcgctgtg gaaagaaatg cgcgagattt attacgggcg caatatgcca
    15061 gctgtttaac ggctctggcg gagctccagg ctccgccaga tttatttact tctgctgcac
    15121 gaaattgcgg taagccgcca cgacctgcag aaaatcctca acgccgcata gcgacaggct
    15181 ttcttcgtcg tagtagttca tcccctcttc catttcatcg ccagcgaatt ccagttgatt
    15241 ggcgcgaacc atcacctctt caccgtccat ccacagggtg tattcatgcc ctgcccgttg
    15301 ccaggaccgt tcgctacctt tcagtgcgtg cgctgcttgt tccacttcat caagcaaggc
    15361 caggttttct ttcacctctt cattaaacca gtgcccgacc acttcatgcc ccatggacat
    15421 acgcaccttt accactccgg taatatcgcg cagaaattcg taatccatag tgtgttcctc
    15481 tgctcccggc aatgcgccga aatcgcattg cgccattgat gtaattatcg cagccttacc
    15541 ggagaaaaac agcggaacgg cgatggttta gaaataaaaa atgcccggga atctcattca
    15601 ccgggcattg tgtcgtttat gcgcagcgcg tgcgcttact tattacaccg cagtctggaa
    15661 aatcacccca tcggcttttt cggtgtactg agaaagttgg ttgaagttca gataacgata
    15721 agtatcaacg gcagttttat ctacttgcgc aacgtaggtc tggtactctt ccggcgtcgg
    15781 cagtttgcca atcagcgccg caacagccgc cagttctgca gaagccagga agacattcgc
    15841 gccagtcccc agacggttcg ggaagttacg ggtagaggtg gaaaccaccg tcgcaccgtc
    15901 tgctacacgc gcctggttac ccatacacag agaacagccc gggatctcga tacgagcccc
    15961 gctcttaccg aagacgctgt agtagccttc ttcagtcaac tgtgcggcgt ccatacgggt
    16021 tggcggtgcc acccacaggc gggtcggcaa ctggccttta tgcgcatcca gcagtttacc
    16081 cgcagcacgg aagtgaccga tgttagtcat gcaagaaccg ataaatactt cgtcgatctt
    16141 ctcgccctgt accgcagaca gcggacgcgc gtcatccggg tcgttcggag cacacaggat
    16201 tggctcttta atatccgcca gatcgatgtc aatcactgcc gcgtattccg catctgcatc
    16261 tgcttccagc agctcaggat tcgccagcca tttttccatg ccctgaatac gacgttccag
    16321 ggtacgacga tcgccgtaac cttccgcgat catccacttc agcaggacga tattagagtt
    16381 aaggtattcg atgattggtt ctttgttcag cttgatagta caaccagcgg cagaacgctc
    16441 agcggacgca tcggtaagct caaaggcctg ctcaactttc agatccggca gaccttcaat
    16501 ttccagaatg cggccagaga agatgttttt cttgcctttc ttctcaacgg tcagcagacc
    16561 ttgtttgatg gcatacagcg gaatggcgtg taccagatcg cgcagggtga tgcccggctg
    16621 cattttgcct ttgaagcgca ccagaacgga ttccggcata tccagcggca ttacgcctgt
    16681 tgcggctgca aatgccacca gaccagaacc cgccgggaaa gagataccga tcgggaaacg
    16741 ggtatgggag tcacctccgg taccgacggt atccggcagc agcatacggt tcagccagga
    16801 gtggattacg ccgtcacccg gacgcagcga cacaccgcca cggttcataa tgaagtccgg
    16861 cagcgtgtgg tgcgtgttca cgtcaactgg cttcggatac gccgcggtgt ggcagaaaga
    16921 ctgcatcacc aggtcagccg agaagcccag gcacgccagg tctttcagtt catcacgggt
    16981 catcgggccg gtagtgtcct gtgaacctac agaagtcatt ttcggttcgc agtacgcgcc
    17041 cggacgaatg cctttcacgc cacaggcacg gcctaccatt ttctgcgcca gcgagaagcc
    17101 gcgatcgctc tcagcgacat ctttcgcctg acggaacaca tcactgtgcg gcagaccaag
    17161 tgcttcacgc gctttggtgg tcaggccacg cccgataatc agcgggatac ggccgccagc
    17221 acgcacttca tcaatcagca cgtcggtttt cagttcgaag gtcgccagca gttcgcctgt
    17281 ttcgtggtta cgcacttcac ctttgtacgg gtaaacgtca atcacgtcgc ccatgttcag
    17341 gttagagacg tcgacttcga ttggcagtgc acccgcgtct tccatcgtgt taaagaagat
    17401 gggtgcaatt ttaccgccga ggcacaaacc accgccgcgt ttgttcggca catgtggaat
    17461 atcatcgccc ataaaccaca gaacggagtt cgtggcggat ttacgcgatg aacccgtacc
    17521 cacaacgtca ccgacgtacg ccagcgggaa acctttctgt tgcagagctt caatctgttt
    17581 gatcgggcca acaacacctg gctggtctgg ctcaatacct tcacgggcgt ttttcagcat
    17641 cgccagcgcg tgcagtggga tatccgggcg cgaccacgca tccggtgccg gagaaaggtc
    17701 atcggtgttg gtttcgccag tgactttgaa gacggtaacg gtcagttttt cagccagcgc
    17761 cgggcgattc agaaaccatt cggcatccgc ccaggactgc attacctgct tcgcatattc
    17821 gttgcctgct ttcgctttct cttctacgtc atagaagtta tcgaacatca gcagtgtgtg
    17881 agaaagtgct ttggcagcga tcggtgccag tttggcatca tccagcgcgt cgatcagcgg
    17941 atgaatgttg taaccaccct gcatggtgcc cagcagttcg atggcttttt ccggagtcag
    18001 cagaggggat ttggcttcgc ctttcgcgat agcagccagg aagcctgctt tgacataggc
    18061 ggcttcatcg acgccagggg gaacacggtt ggttaacaga tctaacagga attcttcttc
    18121 gcccgcgggc gggtttttca gcagctctac aagtgcggcc atttggtttg catccagggg
    18181 tttgggcgca atcccctcag cggcacgctc agctacgtgc ttacggtatt cttctagcac
    18241 gacggttctc ctcgctctca ttgtcatagt gcggcaggcg attctcttca cgctcctgtg
    18301 agacagcagt ttgtagggta aatgcccagt accgcaacgg gcagaatagc aggattttga
    18361 cgacgtgtta atctgtttac aaaaaagcaa cataaaaaag tggctgaatc gttaaggatg
    18421 gtctagagat tgtttccctc cgtaaaaact tcgttaattc gcatggtgat aatcaccttt
    18481 ctcaacgaat cccaagatta gtcaaaattt aaactaccgc ctctttatac tcggattaac
    18541 agcacctgcg ggtggcagtt cgccgaccat tgcgatttcc ttgagatccg aattatgaaa
    18601 atgactttgc cgtttaaacc ccatgtgctg gcactaattt gcagtgccgg gctttgtgcc
    18661 gcctctgccg ggctatatat aaaaagccgc acagtggaag cggctgtgga aacgcaatcg
    18721 acacaactgg ctgtgtctga cgctgccgcg gttacgcttc ctgcaacggt ttccgcgcct
    18781 cccgtaacac ccgccgtcgt taaatccgca ttcagcactg cacaaataga tcaatgggtc
    18841 gcgcccgtcg cgctgtatcc cgacgcccta ctttcgcagg tgctgatggc atcaacctat
    18901 ccggcaaacg ttgctcaagc agtgcaatgg tcgcacgata atccacttaa acaaggcgat
    18961 gctgctattc aggcggtatc tgaccagccg tgggacgcca gcgttaaatc actggtggcc
    19021 tttccacaat tgatggcatt gatgggcgaa aacccgcaat gggtgcaaaa cctgggcgat
    19081 gcttttctgg cgcagccgca ggacgtgatg gactcggtac agcgattgcg gcaactggcg
    19141 caacaaaccg gttcgctgaa gtcatcaaac gaacaaaaag ttattaccac aacgaagaaa
    19201 gctgtaccgg taaaacagac agtcacggca cccgtcatac catccaatac cgttttaact
    19261 gccagccccg tcattacaga gcctgcaaca accgtcattt ccattgagcc cgccaatcct
    19321 gatgtggtct atattcccaa ctacaaccca accgtggttt acgggaactg ggccaatact
    19381 gcgtatccgc cggtttatct gccaccacca gccggagaac cgtttattga cagctttgtg
    19441 cgcggattcg gctatagcat gggtgttgct accacgtacg cactattcag cagcatcgac
    19501 tgggatgacg acgatcatga ccatcatcat catgacgatg atgattatca tcaccacgat
    19561 ggcggtcatc gtgacggtaa tggctggcaa cacaacggcg acaacatcaa tatcgacgtc
    19621 aacaatttca accgtatcac cggtgagcat cttactgata agaatatggc atggcggcac
    19681 aatccaaact accgtaatgg tgtgccctat catgatcagg atatggcaaa gcggtttcat
    19741 caaaccgatg tcaacggcgg aatgagtgcc acgcagctac ctgctccaac acgcgacagc
    19801 cagcgtcagg cggcagcaag tcagtttcag caacgaacac acgccgcccc cgtcattaca
    19861 cgagataccc aacgtcaggc agcggcacag cggtttaatg aagctgaaca ctatgggagc
    19921 tatgacgact tccgcgactt cagccgtcgc caacccctga cccagcaaca aaaggacgcc
    19981 gctcgtcagc gttatcagtc agcttctcct gagcagcgcc aggcagttcg cgagagaatg
    20041 cagactaacc cgcagatcca gcagcgaaga gaggcagcgc gtgagcgcat tcagcccgcc
    20101 tcgcctgagc agcgccaggc agtccgcgag aaaatgcaga ctaacccaca gatccagcag
    20161 cgaagagacg cagcgcgtga gcgtattcag tcagcctcgc ctgagcagcg ccaggtgttt
    20221 aaggaaaaag tacagcagcg cccactgaac caacagcaac gtgataacgc ccgccagcgt
    20281 gttcaatcag catcacctga acaacgtcag gtttttcggg agagagttca ggagagccgc
    20341 ccacaacgtc taaacgacag taaccgtact gccagattga ataacgatca acggtcagca
    20401 gtacgcgaac gtctctctga gcgcggagca aggcgactgg aaaggtaaat tacagacgta
    20461 aaaaaagcgg cgtggttagc cgctttttta attgccggat gttccggcaa acgaaaaatt
    20521 acttcttctt cgctttcggg ttcggcaggt cggtaatgct accttcgaac acttctgccg
    20581 ccaggcccac agactcgtgc agagtcgggt gcgcatggat ggtcagcgcg atgtcttcag
    20641 cgtcacaacc catttcgatt gccaggccga tttcacccag cagctcgccg ccgttggtac
    20701 cgacaatcgc accaccgatc acacggtgag attctttgtc gaaaatcagc ttggtcatac
    20761 cgtctgcgca gtcggaagcg atagcacgac cagaagcagc ccacgggaag gtggcggttt
    20821 catagctgat gcctttctct ttcgcttctt tctcagtcag acctacccat gcaacttctg
    20881 gttcggtata ggcgatagac gggataactt tcggatcgaa gtagtgtttt ttaccggcga
    20941 taacttcagc ggcaacgtga ccttcgtgaa cacctttgtg tgccagcatc ggctgaccaa
    21001 cgatatcgcc gatagcaaag atgtgcggta cgttggtacg cagctgtttg tcaacgcgga
    21061 tgaaaccacg gtcgtccact tccacgccag ctttgcctgc gtcgaggttt ttaccgttcg
    21121 gcacacgacc aatcgctacc agcacggcgt cgtaacgctg cggttcagcg ggtgcttttt
    21181 tgccttccat cgtcacataa ataccgtctt ctttcgcttc aacggcggta actttggttt
    21241 ccagcatcag gttgaatttc ttgctgatac gcttggtgaa gactttaacg atgtctttgt
    21301 cagctgccgg gataacctgg tcgaacattt caaccacgtc aatctgtgaa cccagcgcgt
    21361 ggtatacggt gcccatttcc agaccgatga taccgccacc cattaccagc aggcgttctg
    21421 gtacttcttt cagttccagc gcgtcagtgg agtcccagat acgcggatct tcatgcggaa
    21481 taaacggcag ttggatcggg cgagaacccg ctgcaatgat cgcgttgtcg aagttgatca
    21541 cggttttacc gttctcacct tcaacttcca gggtgttagc cccggtaaat ttacccagac
    21601 cgttgaccac tttgactttg cggcctttcg ccataccagc cagaccaccg gtcagctgat
    21661 tgatcacttt ctctttccag gtacgaatct tgtcaatatc ggttttcggt tcgccgaaga
    21721 cgataccgtg ttcagccagc gctttggctt cttcgataac ttttgctacg tgcagcagtg
    21781 ctttagaagg gatacagccg acgttcaggc aaacaccgcc aagggtgttg taacgttcta
    21841 cgattacggt ttccagacct aaatcagcgc aacggaaggc agcggagtaa cctgcggggc
    21901 ctgccccaag taccacgacc tgagttttga tttcagtact catcatgacc tctatatatt
    21961 tatctccggc ggtcataccc gtcgtctttc aggccgcagc ggcgttagct tcacgccctc
    22021 accccagtca cttacttatg taagcccctg gggattctgg cgcttgctac cttgctgcac
    22081 ccagaaatcc atagggtatg tgaagtaata ccctaaccac caccgggtcg ttctatccgt
    22141 cggtatttta caaaattgtt aacaattttt aaacaacaaa cggcaaccga tttgtctatt
    22201 cgctaataac ctcaatacat tcatgagatt accagaaaaa agccggccgt tgggccggct
    22261 cttttactta catcaccaga cggcgaatgt cagacagcgt gttgttaatg atggtaatga
    22321 aacgggcacc atcagcaccg tcgatcacgc ggtggtcgaa ggagagagaa atcggcagca
    22381 tcagacgcgg cacgaactct ttaccattcc acaccggctc catcgcggac ttggaaacgc
    22441 cgaggatagc cacttccggc gcgttcacaa tcggcgcgaa gtgggtagta cccaggccgc
    22501 cgatgctgga gatggtgaag caaccgccct gcatttcgcc cgcagtcagc ttaccgtcac
    22561 gcgctttctt agaaatagtc atcagctcgc gagacagctc gatgatgcct ttcttgttga
    22621 cgtctttgaa taccggaaca accagaccgt tcggggtatc caccgccaca ccgatgttga
    22681 tgtatttctt cagggtcaga cgctgaccgt cttccgacag cgaactattg aagcgaggca
    22741 tctgctcaag agctgcagca acggctttca tgatgaagac aaccggggtg atcttcacat
    22801 ccagcttacg tttcgccgct tcttcgttct gctgtttacg gaacgcttcc agctcggtga
    22861 tatcggtttt gtcgaagtgg gtaacatgcg ggatcatcac ccagttacgg ctcaggttag
    22921 caccagaaat tttctggata cggcccagtt ccacttcttc gatttcacca aacttgctga
    22981 agtccacctt cggccacggc agcatgcctg ggataccgcc gccagtcgcc gccggagctg
    23041 cttctgcacg tttgatagct tctttcacgt aagcctgaac gtcttcgcgc aggatacgac
    23101 ctttacggcc agtgcccttc actttcgcca ggttaacacc aaactcgcgt gccagacggc
    23161 ggatcagcgg agtcgcgtga acataagcgt cgttttcagc aaattcagat ttgccttccg
    23221 ctttcgcagc tggtgctgct gccggggctt cagcttttgc tgccggtgcc ggcgctgccg
    23281 cttcctgttt cgcaggagct gccgcaggcg ctgcgccttc aacttcgaag atcataatca
    23341 gcgagccagt tttcacttta tcgccaacgt tgactttcag ttccttcacg acgcctgcaa
    23401 acggcgccgg aacttccata gaagctttgt cgccttctac ggtgatcagt gactgttcag
    23461 cggcaacttt gtcgcccact ttcaccatca cttcagtcac ttcaacttcg tcaccgccga
    23521 tatccggaac gttaacttct ttcacgccag ctgctggtgc aggggccgct gccggagccg
    23581 cttcctgttt agccgccgga gctgccgcgc ctgcttcacc cgcgacttcg aagaccataa
    23641 tcagcgagcc ggtagacact ttgtcaccca cgttcacttt gatctctttc acggtgccag
    23701 caaacggagc cggaacttcc atagaagctt tatcgccttc tacggtgatc agcgactgtt
    23761 cagcttcaac tttatcgcca actttcacca ggatttcggt cacttcaact tcgtcgctgc
    23821 cgatatccgg aacgttaacg tcttttgccg ccgcagccgc gggtgctgct gccggagctg
    23881 cttctttctt ctcttctgcc tgagcaggtg cagcgtctgc tgcaccgtcg gcggaatcga
    23941 aaatcataat cagtgcgccg gtctgggttt tatcgccaac agagactttg atctctttaa
    24001 cgatacccgc ctgcggagac ggaacttcca tagaggcttt gtcgccttct acggtgatca
    24061 gcgactgttc ggcttcaact ttgtcgccca ctttgaccag gatctcggtg atttcaactt
    24121 catcagcccc gatgtccggt actttgattt cgatagccat tattctttta cctcttacgc
    24181 cagacgcggg ttaactttat ctgcatcgat gttgaatttg gcgattgcgt cagcaaccac
    24241 tttcttatcg atttcgccac gtttagccag ttcgcccagc gccgcaacca cgacataaga
    24301 agcatcaact tcgaagtggt gacgcaggtt ctcacggctg tcggaacgac cgaagccatc
    24361 agtacccagt acgcggtagt cgtcagccgg tacgtaagta cggacctgct cagcgaacag
    24421 tttcatatag tcggtagatg ccactgccgg agcgtcgttc atcacctgag cgatatacgg
    24481 aacgcgcgga gtttccagcg gatgaagcat gttccagcgt tcacaatcct gaccatcacg
    24541 cgccagctcg gtgaaggagg tcacgctata aacgtcagaa cctacgccgt aatctttcgc
    24601 caggatctct gccgcttcac ggacgtgacg caggatagaa ccggagccca gcagctgaac
    24661 tttacctttg ctaccttcaa tggtttcgag tttgtagata cctttacgga taccttcctc
    24721 agcaccttcc ggcattgccg gcatgtggta gttttcgttc agcgtggtga tgtagtagta
    24781 aacgttctct tgtttttcac cgtacatacg ctccagaccg tcatgcataa tgacagcaac
    24841 ttcgtaagcg taagccgggt cgtaagagat acagttcgga atagtcagcg actgaatgtg
    24901 gctgtgacca tcttcatgct gcagaccttc tccgttcagg gtggtacgac cggaagtacc
    24961 gccgatcagg aagccacgcg cttgctggtc gccagccgcc cagcacagat cgccaatacg
    25021 ctggaagccg aacatcgagt aatagatgta gaacgggatc atcggcagat tgttggtgct
    25081 gtaagaggtc gccgctgcca gccaggaaca acctgcgccc agctcgttga tcccttcctg
    25141 cagaatctga cctttctcat cttctttata gtaagcaacc tgctcgcggt cctgcggggt
    25201 gtactgctga ccgttcgggc tgtaaatacc aatctgacgg aacagacctt ccataccgaa
    25261 agtacgcgct tcgtcggcga tgatcggtac cagacgatct ttgatcgact tgttcttcag
    25321 cattacgttc agggcacgaa cgaaagcgat agtggtagag atctctttgc tctgctcttc
    25381 cagcagcgcg ccgaagtctt gcaggctcgg cagctcaagc ttctcggtga agttcggctg
    25441 acggcttggc agataaccgt gcagtttctg acgctgtgcg tgcagatagg tatgctcttc
    25501 agaaccttcc gggaaggtga tgtacggcag tttttcgata tctgcatcag acaccggcac
    25561 attgaaacgg tcgcggatgt gacgcacgcc gtccatgttc attttcttaa cctggtgcgc
    25621 gatgttttta ccttcagccg cgtcgcccat gccgtaacct ttaatggaat gagcaaggat
    25681 tactgtcgct ttgcctttgg tttcctgcgc tttcttgaat gcagcgtaga ttttcttcgg
    25741 atcgtgacca ccacggttca gtgcccagat ctgctcgtca gtccagtctg caaccagtgc
    25801 tgcggtttca ggatatttac cgaagaagtg ttcacgaacg tacgcaccat ctttcgattt
    25861 gaaggtctgg tagtcgccgt caacggtttc gttcatcagc tggatcagtt taccgctggt
    25921 atctttacgc agcagttcat cccaacggct accccacatc actttgatca cgttccagcc
    25981 agcaccttcg aagatgcctt ccagttcgtt gatgatcttg ccgttaccgg tgaccgggcc
    26041 gtcaagacgc tgcaggttac agttgataac gaagaccagg ttatccagtt tttcacgggt
    26101 agcgatggtg atcgcacctt tggattccgg ttcgtccatt tcaccgtcgc cgaggaacgc
    26161 gtaaacggtc tgtttagagg tatctttcag gccacggtgt tccagatatt tcaggaattt
    26221 agcctggtaa atagcaccaa tcggccccag ccccatagat acggtcggga actgccagaa
    26281 ttccggcatc agtttcgggt gcggatagga agagaggcca ttgccgtgaa cttcctgacg
    26341 gaagttatcc agctgctcct gagtcagacg accttccagg aaagcacgag cgtacacgcc
    26401 cggggagatg tggccctgga agtaaaccag gtcgccgcca tcctgctcgt tgcgtgcacg
    26461 gaagaagtgg ttaaagcaca catcataaat ggttgcggaa gactggaagg acgccatatg
    26521 gccgcccagt tcgaggtctt ttttcgacgc acgcagcacc gtcatgatgg cgttccagcg
    26581 gatagctgaa cgaatacggc gttccagttc cagattaccc ggatactccg gttgttcttc
    26641 aacggggatg gtgttgatgt agttgctgat acctgtgcct gcggctacgt ttacaccgcc
    26701 tttgcgggct tcagcaagca gttggtcgat cagatactga gcacgctcaa caccttcttc
    26761 acggatgacc gattcgatcg cctggagcca gtcgcgagtt tcgatcggat ccacgtcatt
    26821 tgggaaacgt tctgacatgg gttattcctt atctatctaa taacgttgag ttttctggaa
    26881 cctgtcccat tgaactctcg ccggaaagct caataagaca ggttctacgt ttagttgccg
    26941 cgcttttata tgcgcttgat ttacaacatc ttctggataa tttttaccag taaaatcact
    27001 aattctttcg ttgctccaga cgacgcagag aacgctcacg gcggctctct tcacgacttc
    27061 tgtcgagcaa aatttcttcg ataaaggcca gatggcgatg cgatgcttcg cgcgcttctt
    27121 ccggcttacc ggccataatc gcttcaaata tgcgggtgcg gtgactactc accagcggca
    27181 gcatctcgcg acgcgaatag agcaattcga agttctggcg gacattctgg gccaacatcg
    27241 gctccataca ccttagcaga tgaagcagaa ccacattgtg ggccgcttca gtgacagcaa
    27301 tctgatactg gagtacggcg tttgattccg cgtccagatc gccagactgc tgcgccagtt
    27361 ctatggcgtg atggagttca cggatgcgtt ccttgtcttc atcggtacta cgcagcgcgg
    27421 cgtaataagc ggcgatacct tccagggcgt gtcgtgtttc gagcaagtca tactgtgact
    27481 caggatggtc ggagagcagc tccaccagcg gatcgctgaa gctttgccat aggctgctct
    27541 ggacaaaagt gccgccaccc tggcgacgaa gcaacaagcc cttcgcttcg agacgttgaa
    27601 tcgcctcacg caaggaggga cgggagacgt caaactgttt tgccagttcg cgttccggtg
    27661 ggagtttttc gcccgggcgg agagtgcctt cgaggatcaa aaactccagt tgctgctcaa
    27721 tcacatcgga gagttttggt tggcggattt tgctgtaggc catgagttcc tgtcttaagc
    27781 cacttgccga agtcaattgg tcttaccaat ttcatgtctg tgacgctaaa gtaacaaagt
    27841 attcacctta tgtccataca ggttttgatt gaaatcatga aactgtgcac attttaacaa
    27901 cttgacatat ataacgtttc aaagttgtaa ctatgcacaa atgtagactt tacgtaggaa
    27961 agggattttt taacgattat taaactataa aaatccgaat tgaaccgatt cacttaccaa
    28021 ttttgtgatt tttaattcaa ttaaaacgaa tttaaattca ttctacatat tgagaggggt
    28081 tgaggctgag ctttacaaac ggtttctttt taagcaactc atcttcaacc atgcataaag
    28141 cgggtgcatt cgctgccgca taccattatt cttgatctga cggaagtctt tttgtaacaa
    28201 ttcaaacttc tttgatgtaa acaaattaat acaacaaacg gaattgcaaa cttacacacg
    28261 catcactgcg tagatcaaaa aaacaaccac cgcacgaggt ttcatgatgg aaggtcaaca
    28321 gcacggcgag cagctaaaac gcggccttaa aaaccgccat attcagctta tcgcgctggg
    28381 tggcgcgata gggaccgggt tattcctggg tagcgcctcc gtaatacagt ccgcagggcc
    28441 agggattatc ctgggttacg ccattgctgg ttttatcgcc tttctgatca tgcgtcagct
    28501 gggtgaaatg gtggtcgaag aacctgtcgc aggctccttt agccactttg cttataaata
    28561 ctggggcagt tttgccggtt tcgcctctgg ctggaactac tgggtactgt acgttttagt
    28621 tgccatggct gagctgactg ccgtgggtaa atacattcag ttctggtatc cggaaatccc
    28681 cacctgggtt tctgccgccg tattctttgt ggtgattaac gccatcaacc tgaccaacgt
    28741 taaagtgttt ggcgagatgg agttctggtt tgccattatc aaagttatcg ctgtggtagc
    28801 gatgatcatc ttcggcggct ggctgctatt cagtggcaac agcggcccgc aggcgaccgt
    28861 tagcaacctg tgggatcagg gtggtttcct gccgcacggc ttcaccgggc tggtgatgat
    28921 gatggcgatt atcatgttct cgttcggtgg tctggaactg gtggggatca ccgcagcaga
    28981 agctgataac ccggagcaaa gtataccgaa agcaactaac caggttatct accgcatcct
    29041 gattttctat attggttcgt tagccgttct gctctcactg atgccgtgga cccgcgttac
    29101 cgccgatacc agtccgtttg tgctgatctt ccacgagtta ggcgatacct ttgtggcgaa
    29161 tgcgctgaac atcgtggtac tgactgcggc gctctccgtg tacaacagct gcgtatattg
    29221 caacagccgt atgctgtttg gtctggcaca acagggtaat gcgccaaaag cgctggcgtc
    29281 tgtcgataaa cgtggtgtac cagtaaatac cattctggtg tctgcactgg taacggcgtt
    29341 gtgcgtactg attaactacc ttgccccaga gtccgctttc ggactgttaa tggcgctggt
    29401 ggtatctgca ctggtaatca actgggcgat gattagcctg gcgcatatga aattccgtcg
    29461 cgccaagcag gaacaaggcg tggtaactcg cttccctgct ctgctttatc cgctgggtaa
    29521 ctggatctgc ctgctgttta tggcggcggt actggtgatt atgctgatga ccccaggaat
    29581 ggcgatttcg gtatacctga tcccggtatg gctgatcgtg ttaggtatcg gctatctgtt
    29641 taaagagaaa accgccaaag ccgtaaaagc gcattaatct ctctacgccc tcacccgtac
    29701 agggtgaggg caataatctt tacaccaacg ccccgtaaat cgtcagtagt gcaatcacca
    29761 ccacgaccac gaacgaggtt ttcttcgcca ttgaaaccgc tgccttcggc gtctccacct
    29821 tatcgacatg cggttcacgc gccagagaga actgcgccag acgcgttaac acctgatact
    29881 gcgaagtatg gaagtcaccc agcgaagcaa accaggccgg taacgctttc tccccatgac
    29941 cgatcaaggc atataccaca cccgcaagac gaaccggcac ccaatccagt acatgaagca
    30001 cggcatcaat gccggactgt aaacgatgat gcggcgtctg gtatcgcgcc agccagtatt
    30061 gccatgcacg caaaaacgca taccccatca gcgtaacggg tccccagatt ccccccacaa
    30121 tcaaccagaa cagcggagca agataaaaac gaaagttaat ccacagcaat gcattttgca
    30181 gctcacgcaa atactcacgt tcatcgcagc ctgccgggac gccgtgaatc atggtgagtt
    30241 cgccagccat cgtgacacgg gcatggctat cattacgtga agcagctgtc agataagcat
    30301 gataatgaag acgaacttta cctgcgccaa tacacagcaa accaatcagc agccacacca
    30361 gcagcgtggg aacgttgaac aatactccct gcaatgcgcg taacagtaaa aaagtcacgc
    30421 ccatcgcaat aatggtcatg cctaacgtgc gcccgagaga aaaatgtttc acccgccgaa
    30481 agaacgcttc aagacgatga tcaagctgcc agtgctcgcc caacttaaac aggcgctcga
    30541 aaattaacac cagtaaggtt gtaaatagcg tcatgttgtc tccttgctga ccagcgcacg
    30601 aaaccgcgcc caatcaaatg cagggccggg atcggttttc cgatccggcg caatatcaca
    30661 atggcccgtc atgttattag cgatatctgg atagcgatca atcagtgccc gcgtaaccgc
    30721 cgcaagctgt tgatactgcg catcggtata cgccagcgta tcggtgcctt caagctcaat
    30781 cccaatagaa aaatcattgc agcgttcgcg cccctgatac tgagagactc ccgcatgcca
    30841 tgcacgttta tcgaaaggaa catactggac tatttcacca tcacggcgaa tcaaacagtg
    30901 agcggagacg cgcaaatggg cgatctcagc aaagaaagga tgtgcctgcg gatcaatagt
    30961 tccagtgaat aatgcgtcga tccacggacc gccaaactcg cctggcggca ggctaatatt
    31021 gtgcaccacc agcagggtgg gtgtttcgtc atccgggcgg caatcgtaat gtggtgaggg
    31081 aacgcggcgc gcgccaacca gccacccctg ttctaacaac atgcaggatc tccttatatg
    31141 tggtgctaat acccggttca gagtagcatg tttctacctt atgattcgtt agctatctgg
    31201 agttttaaca tgccgcctcg ccgctataac cctgacaccc gacgtgacga gctgctggaa
    31261 cgcattaatc tcgatatccc cggcgcggtg gcccaggcgc tgcgggaaga tttaggcgga
    31321 acagtcgatg ccaacaatga cattacggca aaacttttac cggaaaactc tcgctctcat
    31381 gccacggtga tcacccgcga gaatggcgta ttttgcggca aacgctgggt tgaagaggtg
    31441 tttattcaac tggcaggcga cgatgtcacc ataatctggc atgtggatga cggcgatgtc
    31501 atcaacgcca atcaaccctt gttcgaactt gaaggcccat cccgcgtgct gttaacgggc
    31561 gaacgcactg cgcttaattt tgtgcaaacc ctttcaggag ttgccagtaa ggtacgccac
    31621 tatgtcgaat tactggaagg caccaacacg cagttgttgg atacgcgcaa aaccttacct
    31681 ggcctgcgtt cagctctgaa atacgcggta ctttgcggcg gcggagcgaa tcaccgtctg
    31741 gggctttctg atgccttcct gatcaaagaa aatcatatta ttgcctccgg ctcagtgcgc
    31801 caggccgtcg aaaaagcgtc ctggctgcac ccggatgcgc cagtagaagt cgaagtagag
    31861 aatctggaag aacttgatga agccctgaaa gcaggagccg atatcatcat gctggataac
    31921 ttcgaaacag aacagatgcg cgaagccgtc aaacgcacca acggcaaggc gctactggaa
    31981 gtgtctggca acgtcactga caaaacactg cgcgaatttg ccgaaacggg cgtggacttt
    32041 atctccgtcg gtgcgctaac taaacacgta caagcactcg acctttcaat gcgttttcgc
    32101 taattgctga cagatttatc cgctccatcg cgggcggata atctcccctt ccccaacttt
    32161 cttcgtaacg cctcgcaaat ttcttgtctt tcgctgcaac gaaataaaaa acctcggaag
    32221 ctgctttccc gtttcgtttt ttgcccctcg cttgtgctga tggcgtttgc caaagtagcg
    32281 ccaaccaaat caaggagcga aacagatgga caagcaacgc ggttttacac ttatcgaact
    32341 gatggtggtt attggcatca ttgccatttt aagcgccatt ggtattcccg cttatcaaaa
    32401 ctacctgcgc aaagctgcac tcaccgacat gctacaaacc tttgtgcctt accgtaccgc
    32461 cgtagagttg tgcgcgctgg aacatggtgg
//
LOCUS       DAJVUJ010000028        31263 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.028, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000028 DAJVUJ010000000
VERSION     DAJVUJ010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31263
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.028"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(<1..3188)
                     /locus_tag="OQQ48_001220"
     CDS             complement(<1..3188)
                     /locus_tag="OQQ48_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HCS1952064.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISCYKNVPVTTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIKLQRSKRGIEWGLYAISPLNGYRLAIREIGKCN
                     ALLDDAVALMPATAIQGVLHGINPERLTIELSDADGNIVLSYQEHQPQELPLLDVAKA
                     PLAAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARV
                     QREKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTL
                     AAEMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYNKAIAFWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLASAGLGEVAQYES"
     gene            3306..4499
                     /locus_tag="OQQ48_001221"
     CDS             3306..4499
                     /locus_tag="OQQ48_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCS1952065.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(4563..5816)
                     /gene="glyA"
                     /locus_tag="OQQ48_001222"
     CDS             complement(4563..5816)
                     /gene="glyA"
                     /locus_tag="OQQ48_001222"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCS1952066.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            6144..7334
                     /gene="hmpA"
                     /locus_tag="OQQ48_001223"
     CDS             6144..7334
                     /gene="hmpA"
                     /locus_tag="OQQ48_001223"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HCS1952067.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISSGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIYYECFGP
                     HKVL"
     gene            complement(7379..7717)
                     /gene="glnB"
                     /locus_tag="OQQ48_001224"
     CDS             complement(7379..7717)
                     /gene="glnB"
                     /locus_tag="OQQ48_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HCS1952068.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(7778..9112)
                     /gene="glrR"
                     /locus_tag="OQQ48_001225"
     CDS             complement(7778..9112)
                     /gene="glrR"
                     /locus_tag="OQQ48_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HCS1952069.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(9102..9815)
                     /gene="qseG"
                     /locus_tag="OQQ48_001226"
     CDS             complement(9102..9815)
                     /gene="qseG"
                     /locus_tag="OQQ48_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HCS1952070.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGCWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(9980..11407)
                     /gene="qseE"
                     /locus_tag="OQQ48_001227"
     CDS             complement(9980..11407)
                     /gene="qseE"
                     /locus_tag="OQQ48_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HCS1952071.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSS
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGVVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(11965..15852)
                     /gene="purL"
                     /locus_tag="OQQ48_001228"
                     /gene_synonym="purI"
     CDS             complement(11965..15852)
                     /gene="purL"
                     /locus_tag="OQQ48_001228"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HCS1952072.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRILGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRILHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGINADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            16112..17668
                     /gene="mltF"
                     /locus_tag="OQQ48_001229"
     CDS             16112..17668
                     /gene="mltF"
                     /locus_tag="OQQ48_001229"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HCS1952073.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLCNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(17665..18201)
                     /gene="tadA"
                     /locus_tag="OQQ48_001230"
     CDS             complement(17665..18201)
                     /gene="tadA"
                     /locus_tag="OQQ48_001230"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HCS1952074.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(18226..18861)
                     /gene="yfhb"
                     /locus_tag="OQQ48_001231"
     CDS             complement(18226..18861)
                     /gene="yfhb"
                     /locus_tag="OQQ48_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HCS1952075.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDKPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            19070..19918
                     /locus_tag="OQQ48_001232"
     CDS             19070..19918
                     /locus_tag="OQQ48_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCS1952076.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            19974..20234
                     /gene="yfhL"
                     /locus_tag="OQQ48_001233"
     CDS             19974..20234
                     /gene="yfhL"
                     /locus_tag="OQQ48_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HCS1952077.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            complement(20428..20508)
                     /gene="shoB"
                     /locus_tag="OQQ48_001234"
     CDS             complement(20428..20508)
                     /gene="shoB"
                     /locus_tag="OQQ48_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HCS1952078.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(20928..21308)
                     /locus_tag="OQQ48_001235"
     CDS             complement(20928..21308)
                     /locus_tag="OQQ48_001235"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCS1952079.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            complement(21308..22039)
                     /gene="pdxJ"
                     /locus_tag="OQQ48_001236"
     CDS             complement(21308..22039)
                     /gene="pdxJ"
                     /locus_tag="OQQ48_001236"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HCS1952080.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKMRDACKRLTDAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            complement(22051..22779)
                     /gene="recO"
                     /locus_tag="OQQ48_001237"
     CDS             complement(22051..22779)
                     /gene="recO"
                     /locus_tag="OQQ48_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCS1952081.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTYCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            complement(22791..23696)
                     /gene="era"
                     /locus_tag="OQQ48_001238"
     CDS             complement(22791..23696)
                     /gene="era"
                     /locus_tag="OQQ48_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCS1952082.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            complement(23693..24373)
                     /gene="rnc"
                     /locus_tag="OQQ48_001239"
     CDS             complement(23693..24373)
                     /gene="rnc"
                     /locus_tag="OQQ48_001239"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCS1952083.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            complement(24646..25620)
                     /gene="lepB"
                     /locus_tag="OQQ48_001240"
     CDS             complement(24646..25620)
                     /gene="lepB"
                     /locus_tag="OQQ48_001240"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCS1952084.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            complement(25636..27435)
                     /gene="lepA"
                     /locus_tag="OQQ48_001241"
     CDS             complement(25636..27435)
                     /gene="lepA"
                     /locus_tag="OQQ48_001241"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCS1952085.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            complement(27633..28112)
                     /gene="rseC"
                     /locus_tag="OQQ48_001242"
     CDS             complement(27633..28112)
                     /gene="rseC"
                     /locus_tag="OQQ48_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HCS1952086.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            complement(28109..29065)
                     /gene="rseB"
                     /locus_tag="OQQ48_001243"
     CDS             complement(28109..29065)
                     /gene="rseB"
                     /locus_tag="OQQ48_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HCS1952087.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGDEISYFEPGLEPFTL
                     NGDYIVDSLPSLVYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            complement(29065..29715)
                     /gene="rseA"
                     /locus_tag="OQQ48_001244"
     CDS             complement(29065..29715)
                     /gene="rseA"
                     /locus_tag="OQQ48_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HCS1952088.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            complement(29748..30323)
                     /gene="rpoE"
                     /locus_tag="OQQ48_001245"
     CDS             complement(29748..30323)
                     /gene="rpoE"
                     /locus_tag="OQQ48_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HCS1952089.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            complement(30320..30475)
                     /gene="rseD"
                     /locus_tag="OQQ48_001246"
     CDS             complement(30320..30475)
                     /gene="rseD"
                     /locus_tag="OQQ48_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HCS1952090.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            30731..>31263
                     /locus_tag="OQQ48_001247"
     CDS             30731..>31263
                     /locus_tag="OQQ48_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCS1952091.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRR"
BASE COUNT         7467 a         8384 c         7891 g         7521 t
ORIGIN      
        1 gactcatatt gcgcaacctc cccgagtccc gcggacgcca gcatcgccac catcaggcat
       61 ttttctttat gctgctgttg taaatcgcca tacagcgata acaggtcagg ttgtgaaaca
      121 gcaaagaaat ccgcctcaat actggttttc gccatctctt gcgcccactg tttcatttca
      181 ctaaacagtt gctgtgcggt ttgctgttca cccagcagtc gcagcgccat tccttgccag
      241 aagagataat caaccggctg atcgttgtaa taactgtgga tgttaatggt gcgatcgccg
      301 gtcgccgcca gacgtaaaca acgcatcgct tcagtttcat cgccctgcgc gttggcgcat
      361 atcgcctgcc agaaccagat gtcgttatca gtttgccccg gtaaacggcc ttcgcttaaa
      421 ttctccggat aatgcagcgc ggcatgaagc agttcgctgg cctgctgcgg ctctctggca
      481 tcaagatgct gccaggcgcg taataactgg ttgaggataa actgactggt gaccttccct
      541 tccccgcctt cccacgggtg gaatttacgc gtggcgagaa tgtccgccgc tttgtctgcc
      601 tgacccgtga gatgccacaa attgagcagt tctgcggtca tgtcgtcgcg tttcagcgca
      661 atttccagat tattttccag acgcgccagt cgtttctccg gtgtggctcc acttaactta
      721 tcaagcaaat cccgttcgaa aagcagacgt gcatcctgcg gcgcaagctg ataagcatta
      781 tcaagataac gcgcggccag ctcataatcg tgttgcttat tccacgcatg gatcgctaac
      841 ccgcgccagc cgtcggcaaa ctccggcgac atctctacgc aacgttgcca aaaggcaatg
      901 gctttgttgt agctacgttt gttgtagtag aagcaggcca gtaaatggcg agcaaaccag
      961 cactcttcaa tactctccag cgccgccact tcttccagcg tattcgggaa acggacaaac
     1021 tgcgggaaga catcaatggc ttttgcgacc agttcgccac gttcggcttt cggcagcaag
     1081 ctggcttgca ggtaaagcgg cagcgtgcgc tggcagtcca gtgcgttcag catctctgcc
     1141 gccagggtgg gcattcccca gttaatcagt tgcccggcgg tcatcagggc gttaacgtcg
     1201 cgtccctgac acagaccgcg ccactgcgcg agagctgatt cgctacgacc atcgaaccag
     1261 ttcagccacc acagagtggc gttcagcgga taatcgcgca gcagtttctc gcgctgcaca
     1321 cgcgcgttgt cctgacgacc acttaacacc agcagcagat tatgcaggca aagcacttcc
     1381 tgattggttg ggcaggcgcg aagactttgt tggcaaaaat ccagaccagc gtcgaagtta
     1441 ccattacgcg ccgccagtcg tgccaggcca taatagccac cggctttgct gttgccgctc
     1501 cagaccgcac gccagaaatc ctcttcggct tgttgatatt gtccctgacg ttcgtaagca
     1561 ctggcgcgaa tcaaactcgc ctgtccgcac tgcggatttt tgttcagcgc atgtgcgcgt
     1621 ttcagagcct gagtggcata cgccaccgct tgcgggaaat cggcgcggtt atattccagc
     1681 atcgccagcg ccaggttaca gcggtaatcc agcggatcca gcgccacgcc gcgcaggtag
     1741 taatcgaacg gtgaacggct ggcgtgatga tattgctcca gatgctgacc gataaaccag
     1801 gcttcatctg tactggtaat gtcttgtgct gccagtggcg ctttggcgac gtccagcaac
     1861 ggcaactctt gcggctgatg ttcctgataa ctcagtacaa tattgccgtc ggcatcggag
     1921 agctcaatgg tcagcctttc aggattgata ccgtgcaaca cgccctggat ggcggtcgca
     1981 ggcatcagtg ccacggcatc atcaagtaac gcgttgcatt tgccgatttc gcggatcgcc
     2041 aggcggtatc cgttcaacgg agagatggca tacagccccc actcaatccc ccgcttacta
     2101 cgctggagtt ttatcaccgc atcgcgggag gcattttgca ccatgcccaa agaatgataa
     2161 ggcaggaaat actgctcaaa acgcttctct tcgtaagcat caagccaggt aaaatcgggc
     2221 tggttatcgg caaaaatacc ggtcatcagt tcgatatacg ggccgttatt gtcggtcaga
     2281 ctcttatccc acgcctggcc aaattcactg tgtccccaac tccactgttt tttacctggc
     2341 gcaatatggt ggttggcaac gtgcagcaaa ccgccatctt catcgtgaca ccacgcgccg
     2401 acaaaatcgt actgcgattt ttcggccata tatgaggttg taacaggcac atttttatag
     2461 caagaaatgt ccactccggc ggagtagtcc actttgtagt aagtgccggt ggcgatgggg
     2521 aaagcggaga cggcccgttt gccgtgatca aacaccgcag tcacatccgg tgggaaaacg
     2581 ctctgatgcc cttccccccc tttcactgcc gggttggccc accacaagaa atgacgcggc
     2641 gtggcgttac cgttatagac gcggctggcg atttccagcg ccgcccggtc agggcgcagg
     2701 gtgaaacctg tcatcacctg taaaccatgc atcggctccg tttcgcctac ccacaccgtc
     2761 tgtgcaccgt cttcatgggc ttcgagggtg aaatcaacgg gcataaaggt ggtcgggcga
     2821 tgatgttgcg gccagttaaa ctcaatccca ccggaaatcc acggccccag cagccccacc
     2881 agcgcaggtt taatgacttc attgtgataa acaaaatcgc gctgtttcac tttatcccac
     2941 gcgcgatgca cccgaccgcc cagttccggc aggatcatca ctttgatgta gtcgttttcc
     3001 agccacaccg cctgccagga tttcagggtt ttctgctcgc tcagcgtatc ggtcacgccg
     3061 tagggataaa ccgcgccgga cgatccctga taaacgcgat tttccaggaa catgggatgt
     3121 atatcctgcg gcccggtttc ataggtcggg atctcaacgc gctcttgcca cacttttact
     3181 ggagtcatgg tgccctcaat attaacaaga catactgaat taaaagattt gtggcagtgt
     3241 attgaacaat ctggcaatgt tttcgcggaa taatcacgca attaactaaa caaggtttag
     3301 tgaagatgag agcctgcatt aacaatcaac agattcgcca ccataacaaa tgcgtgattc
     3361 tggaactgct gtaccggcaa aagcgcgcca ataaatcaac gctggcccgg ctggcgcaaa
     3421 tttcgattcc ggcagtcagt aatattttgc aggaactgga aagcgaaaaa cgggtggtga
     3481 atattgacga tgaaagccag acgcgcgggc atagtagcgg tacatggctg attgcgccgg
     3541 aaggtgactg gacgctgtgc ctgaacgtga cgcccaccag tattgagtgt caggttgcta
     3601 atgcttgttt aagtccgaaa ggtgaatttg agtatttaca gattgatgca ccgacgccgc
     3661 aggcgctgct gtccgaaatc gaaaaatgct ggcatcgcca ccgtaaattg tggccggacc
     3721 gcaccatcaa tctggcgctg gcaatccacg gtcaggttga tccggtgacc ggcgtgtcgc
     3781 aaaccatgcc gcaagcgccg tggacaacgc cggttgaggt gaagtatctg ctggaagaga
     3841 agctgggcat tcgggtgatg gtcgataatg actgcgtgat gctggcgctg gcggagaaat
     3901 ggcaaaataa ttcgcaggaa cgggatttct gcgtgatcaa cgttgattac ggcattggct
     3961 cgtcgttcgt gattaacgag caaatttatc gcggcagttt gtatggtagc ggacaaattg
     4021 gtcacaccat cgttaatccg gatggcgtcg tctgcgactg tggacgttac ggctgcctgg
     4081 aaaccgtcgc ctcgttaagt gcattaaaaa aacaggcgcg ggtatggcta aaatcacaac
     4141 cggttaatac tcagcttgat cctgaaaaac tgacaaccgc gcagttaatc gctgcctggc
     4201 agagtggaga accgtggatc accagctggg ttgatcgctc tgccaatgcc attggtttga
     4261 gtctgtataa cttcctcaac atcctcaata ttaatcagat ttggttgtac ggtcgcagtt
     4321 gtgcctttgg tgagaactgg cttaatacta ttattcgcca gacaggattt aacccgttcg
     4381 accgcgacga aggaccgagc gtgaaagcga cgcaaattgg cttcgggcaa ttaagccgcg
     4441 cacaacaggt gctgggaatt ggctatttgt atgttgaggc gcagttacga cagatttgat
     4501 ggcgcgataa cgtagaaagg cttcccgaag gaagcctttt ttcaacagat taaccgtttc
     4561 gcttatgcgt aaaccgggta acgtgcgcag atgtcgagaa ctttaccttt gatgcgctcg
     4621 ataacggctt catcattgat gctgtccagc acgtcacaca tccagccagc cagttctttc
     4681 gcttctgctt ctttgaagcc gcgacgcgta attgccggag tacccacgcg aataccggag
     4741 gtcacaaacg ggctcttcgg atcgttcggt acgctgtttt tgttgacggt gatgttagca
     4801 cggcccagag cggcgtctgc ttctttaccg gtcaggtttt tatcaaccag atcaaccagg
     4861 aacaggtggt tatcagtgcc gccggaaacc actttgtagc cgcgctcgag gaacacttct
     4921 accatcgctt tagcgttttt cgcgacctgc tgctggtaag ttttgaactc aggctccatc
     4981 gcttctttca gagcaaccgc tttaccggcg attacgtgca tcaacggacc gccctgacca
     5041 ccagggaaaa cggcagagtt cagttttttg tacagctctt cgctaccacc tttcgccagg
     5101 atcaggccgc cgcgcggacc cgccagggtt ttgtgagtgg tggtagtaac aacgtgagca
     5161 tgaggaaccg ggttcgggta gacgccagca gcaaccaggc ccgcaacgtg cgccatatca
     5221 acgaacaggt aagcaccgat gctgtcagcg atttcacgca ttttcgccca gtccaccacg
     5281 ccggaatatg cagagaaacc accgataatc attttcggct tgtgttcttt ggcttgtttt
     5341 tccagatcgg cgtagtcgat atgaccggta gcatcgatac cgtaaggaac gatgttgtac
     5401 agtttaccgg agaagttaac cggagaaccg tgagtcaggt gaccgccatg cgccaggttc
     5461 atacccagaa cggtatcacc tggttccagc agcgcggtgt agaccgcaaa gttagcctgg
     5521 gagccggagt gcggctggac gttagcgtag tcagcgccga acagttcttt cgcacgatcg
     5581 atcgccagtt gttcaacgat atcaacatac tcgcaaccgc cgtagtagcg tttgcccgga
     5641 taaccttcag catatttgtt ggtcagctga gaaccctgcg cctgcattac gcgcgggctg
     5701 gtgtagtttt cggaggcgat cagttcgatg tgctcttcct gacgtacttt ttcctgctcc
     5761 atagcctgcc acagttcggc atcataatcg gcaatgttca tttcacgctt taacatccgc
     5821 atctcctgac tcagctaaca ataaaatttt tggcctttat aggcggtcct gttggacaac
     5881 ggcgaacagt ataaccgaat cattgtgcga taacaggtct tgacaaagga atttacgcaa
     5941 acgattacct tcaggctacg caaggctttg gagaataaag agcttgcaac cggaaacgga
     6001 tttcttttca ggtttgtgat gcaaattttt cacttcatca cattctttct gaaaaacacc
     6061 aaagaaccat ttacattgca gggctatttt ttataagatg catttgaaat acatcaatta
     6121 agatgcaaaa aaaggaagac catatgcttg acgctcaaac catcgctaca gtaaaagcca
     6181 ccatcccttt actggtggaa acggggccaa agttaaccgc ccatttctac gaccgtatgt
     6241 ttactcataa cccagaactc aaagaaattt ttaacatgag taaccagcgt aatggcgatc
     6301 aacgtgaagc cctgtttaac gctattgccg cctacgccag taatattgaa aacctgcctg
     6361 cgctgctgcc agcggtagaa aaaatcgcgc agaagcacac cagcttccag atcaaaccgg
     6421 aacagtacaa catcgtcggt gaacacctgt tggcaacgct ggacgaaatg ttcagcccgg
     6481 ggcaggaagt gctggacgcg tggggtaaag cctatggtgt actggctaat gtatttatca
     6541 atcgcgaggc ggaaatctat aacgaaaacg ccagcaaagc cggtggttgg gaaggtactc
     6601 gcgatttccg cattgtggct aaaacaccgc gcagcgcgct tatcaccagc ttcgaactgg
     6661 agccggtcga cggtggcgca gtggcagaat accgtccggg gcaatatctc ggcgtctggc
     6721 tgaagccgga aggtttcccg catcaggaaa ttcgtcagta ctctttgact cgcaaaccgg
     6781 atggcaaagg ctatcgtatt gcggtgaaac gcgaagaggg tgggcaggta tccaactggt
     6841 tgcacaatca cgccaatgtt ggcgatgtcg tgaaactggt cgctccggca ggtgatttct
     6901 ttatggctgt cgcagatgac acaccagtga cgttaatctc ttccggtgtt ggtcaaacgc
     6961 caatgctggc aatgctcgac acgctggcaa aagcaggcca cacagcacaa gtgaactggt
     7021 tccatgcggc agaaaatggc gatgttcacg cctttgccga tgaagttaag gaactggggc
     7081 agtcactgcc gcgctttacc gcgcacacct ggtatcgtca gccgagcgaa gccgatcgcg
     7141 ctaaaggtca gtttgatagc gaaggtctga tggatttgag caaactggaa ggtgcgttca
     7201 gcgatccgac aatgcagttc tatctctgcg gcccggttgg cttcatgcag tttaccgcga
     7261 aacagttagt ggatctgggc gtgaagcagg aaaacattta ttacgaatgc tttggcccgc
     7321 ataaggtgct gtaatttgat gttgccggat ggaaacatcc ggcaaccctt gacgcggttt
     7381 aaattgccgc gtcgtcctct tcaccggtac ggatgcgaat gacccgtgcc acgtcaaaga
     7441 cgaagatttt accgtcaccg attttgccgg tttgcgccgt gcgaataatg gtatcgacac
     7501 aggtatcgac aatgtcgtcc ggtacgacaa tctcaatttt cactttcggc agaaaatcca
     7561 ccatatactc cgcgccgcgg tacagctcgg tatggccttt ctggcgacca aagcctttca
     7621 cttcggtcac cgtcatgccg gtaataccga cttcggccag tgcttcgcgg acatcgtcca
     7681 gcttgaaggg ttttataatc gcatcaatct ttttcatgct attccttgaa aaggtcgcct
     7741 gtcttttgat ctgctaaacg taacacataa cgccaattca ttccttgaaa tcgtttgcat
     7801 ccagctcgtg tcgggaaagc agtttataaa attctgtccg gttgcgcccc gccattctcg
     7861 ccgcgtgggt gacgttgcct ttggtaattt gcagcagctt acgcaaatag ttgagttcaa
     7921 actgattacg tgcctcaaca aaggttggca gcgccgtgtt ttcaccctcc agcgcctgct
     7981 ctactaatgc atcgctaata accggagatg aggtcagcgc cacgcactgt tcaatcacgt
     8041 tgaccaactg gcgcacatta cccggccagc tagcggtcat caggcgtttc atcgcatcag
     8101 tggagaacgc gcggacaaac ggtttatgtc gctctgccgc ctggcgcaac aggtgatttg
     8161 ccagaagcgg aatgtcttct gtgcgctctg ccagagccgg aattttcagg ctgacaacgt
     8221 tgaggcggta atagagatct tcgcggaatt ccccgcgcgc catcgctttt ggcagatcac
     8281 ggtgagtggc agaaataatc cgcacattga tatcaatatc gcggttactg cccagcgggc
     8341 gcactttacg ctcctgcaac acgcgcagca gtttgacctg taacggcgca ggcatatcgc
     8401 caatttcatc gagaaatagc gttccgcctt ccgccgcctg gaataaacct tcgcgattgc
     8461 tgacagcgcc agtaaacgcg ccacgcgcat gaccaaacag ctccgactcc agcaattgct
     8521 cgggcaatgc gccacagtta atagcaataa atggtttgct gttgcgcggg ctggcgttgt
     8581 ggatagcctg ggcgaaaatc tctttcccgg tgccgctctg accgttaatc aaaacgctga
     8641 cgtctgattg cgccaccagc cgcgcctgtt ccagcaaacg cagcatcagc gggctgcggg
     8701 tgacaattgc ctcgcgccag cgttcatcgg tggctggcgc ggattgctcc agcgcatcgt
     8761 caattgcctg atatagcgcg tctttgtcga caggcttggt gaggaaacta aaaacgccct
     8821 gctgtgttgc agcaacggca tcggggatag aaccatgcgc agtaagaata attactggca
     8881 ttcccggctg cactttctgg atttcagcaa atagctgcat accgtccatt tcatccatcc
     8941 gcaggtcgct gataactaaa tctacttttt cgcgattcag tacccgtaat ccttcagcgc
     9001 cactttccgc cgtgaccaca ctgtagcctt cgctggtcag gcgcaggcca agcagtttca
     9061 gcaatcccgg atcgtcatcg accaataata aatgcgcagg tttatggctc atcaggagtg
     9121 acctcatggg tggatggcgc gggcttttca ctctcgtgcg gcgtatccgg cgagaaattt
     9181 ccagccggtt tgcgggtcga gagctggcgt tcaatatcgg tcaggttttc cagcttgcgg
     9241 gtggtgagtt ccagttgctg ctgtagaacg tgatgttgct ggcgcaatgt atccagctcg
     9301 ctgtcgctgg actgctggag tttactgtaa cgttggcgct cttccgccag ttgcagttgc
     9361 agcgcctgac cgtcgcgcca gagttgatac agtgggcgaa cctgtgccgg gatctcggta
     9421 cttaacgctt caatacgcgc gaccagctgg cggcgctcat acggcgtaat tttggcgtcg
     9481 gcgagcaaaa tcccttgttt aaaggtattt tgccagcagc cgtcgtcata ttggcgagct
     9541 tgctgacgcg actgcgcagg cattaaacga tcagcacaat ccatcgcccg cagccagtaa
     9601 agcggattgg tttcggttga tttaccttgc agcgcccaga tgtcgctaca ttcagtagaa
     9661 agatagtcag ccagttgata aaccggaatt ttttcttctg ctggcgtatc aatggctggc
     9721 ttattgtgat tctgcacgca acccagcaat gccagacatg gcagccctgc cagccacagc
     9781 cgtcggggca ataatcgttg aaaaatgtgt cgcatattca ccagacttaa agcctatccc
     9841 agtgggcgta attgttgcag acagtctgga catggacagc gcggagaaac cggagcgtac
     9901 atatcgtacg tgaagatttc gagcactgcc cggagccgaa ctgacaaata aaatagcctg
     9961 ataggatagg ctgttagcat tatttcgtgt ttttcgacga cggtaattca atgcggaaac
    10021 aaacgtcttg cccgctctcg tcgaccagat acagttcccc ttgcatacgg cgaatacaat
    10081 ccctggcaat gcttaatccc agaccgctgc ccttcaccac cccttttcgc tggtggcttc
    10141 cctgaaaaaa gggttcgaag atcatggcgc gttcctcttg cggaatgggc gtgcctgtat
    10201 tgatgacatc aatataaacc cgcgcaccat gtgaactgct gcgaaggcaa atgttaccgg
    10261 attcagcccc gtagtgcacc gcattggagt aaagattatc cagtacgctc atcagcagca
    10321 ttggctccgc caggcaagct gttgctttga gatcgacgtc ggtatgcatc attttagccc
    10381 gtgcgggcag gctatgagca gaaaccactg tctccaccag cggtgctaat tcaacattct
    10441 ccagttccac cgcactgtcc gcctgtttac ggttgtaatc aagcagttgt tcgatcagtt
    10501 tttgcaaatt gcggctgctg ctatcaagaa tgctcaccac ctctttttgc tctggcgtaa
    10561 gcggcccgac aacctggtca gccagtaatt cagtgccctc gcgcatactc gccagtggcg
    10621 tttttaattc atgagataaa tgtcttaaaa attgatggcg ttgggattcc agccatgaca
    10681 ggcgctcact taaccaaaga atacgttgcc caaccgagcg taactcgctc ggtccactga
    10741 acgaaacgct attgcccaga gaacgccctt cccccagacg gttgatcatg cgctcgatat
    10801 ttttcaccgg cccgataatc atccgcgtga aaagtagcac catcaccaga ctcaccagaa
    10861 atagcaccag cgattgccaa ccaaaatatt gcccacgttc ggcgatttca cgctgaagtt
    10921 gctgcccacg agagaacacc actgtgcgcg tggcctgcac catttcggta ttggcactgg
    10981 caaaggcttc aagacgcgcg gcggcggcag catcgggacc actgttgtta cactgaagtt
    11041 gggccagatt gttcaaatcc tgacgcaatg cctggtaaag tttatcgtct ggcagtacgc
    11101 ctgcgtgggc atcgagcatt tcgctgtaac gcttgcgctg gctttgataa accttcgcaa
    11161 gcgtagggtc gtccagcacg caatactgac ggtagctacg ctccatctcc agcgccgcgt
    11221 tggtcatcgc ttcactacgc cgggcatcaa taagcgtagt gcggttaacc agcgccgcct
    11281 gatcgctaag cgcattcagg ctttgccagg cttgccatgc cagcaccaac aggggtagta
    11341 gaattagcaa aaatgccagc attaccagtt gtcgtaatga gcggggaaaa acgggccagc
    11401 gtttcaaggt gttactctcg tcagacgcga atagcctgat gctaaccgag gggaagttca
    11461 gatacaacaa agccgggaat tacccggctt tgttatggaa taaggcggtg cctaactcga
    11521 cgtttcgccc gatggttgat atagctacgc tgatatcaga agttggacgg caggcacctt
    11581 gttgtgcgtc attcgaattt tatgtagcac gtcccgaagg ggctgacata agtcggtgaa
    11641 tgagccactg cttactatta tgcagtaact gtgccaataa agaaaatagt ttggtaacgt
    11701 actgattata cgttgctttg agggatttat ggcttcttcg ctatttgaat gtaaatcagc
    11761 cactgtgtcg ccaaaaagag acagcttaag ataaacttat tagattacaa taaaatcaat
    11821 cacttaagtg tcgccagaaa gcgacacggt aaaccaccca ttgtcgtgat ttacagacac
    11881 aaaaaagctc ccggagttgg gagcttatga tagtggttgg tgcttaatgc cgcatccggg
    11941 ctgcaaaacc aatgaactga tgaattaccc caactgctta cgcgcattgc ggaaaatgcg
    12001 catccatggg ccatcctcgc cccagttttc cggatgccag gagttgctga cagtacggaa
    12061 aacacgttcc gggtgcggca tcataatggt gactcgacca ctttcagtcg tgactgccgt
    12121 aataccgttc ggtgaaccgt tcgggttagc cgggtaggtt tcagtgactt tgccgaagtt
    12181 atcgacatag cgcagtgcca ccagcccttt gctttccagt gccgccagat gcgccgcatc
    12241 acgcacttcc acgcgccctt caccatgaga gacagcaatc ggcatttgcg agcccaccat
    12301 cccctgcaac aacagagacg ggctttgggt tacttcaacc aggctgaaac gcgcttcaaa
    12361 gcgatcggag gtattgcgca caaaacgtgg ccacaactca ctacctggga tcagttcgcg
    12421 caggttagac atcatctggc aaccgttaca tacccccagc gccagcgttt gcggacggtg
    12481 gaagaaggtt gcaaactcat cgcgtacacg gtcattgaac aggattgact tcgcccaacc
    12541 ttcaccggca cccagcacat caccgtagga gaaaccaccg cacgcgacca gggcgtggaa
    12601 atcttccagg cccgtgcgtc cggtcagcag gtcactcata tgcacgtcga tagcatcaaa
    12661 gcctgcacgg tggaaagctg ccgccatttc aacatgcgag ttcacgccct gctcacgcag
    12721 tacggcaact ttcggacgtg cgccagtggc aatatacggt gctgccacat cttcgttgat
    12781 atcgaacgac agttttacat tcaggcccgg atcggcgtcg ttagatttcg cctgatgctc
    12841 ctgatcggca cactccgggt tgtcacgcag gcgctgcatc tgccaggtag tttctgccca
    12901 ccagacacgc aacgtggtgc ggctttcgct gaatacagtc tgcccgttgg cggtaatcac
    12961 aaaacggtca ccggaaaccg cctgccctac ataatggaca caatcagcaa gcccatgctg
    13021 tgccagtacg gactcgaccg cttcacggtc agcggcacga acctgaatca ccgcacccag
    13081 ttcttcgtta aacaacgccg ccaggcgatc gtcgcccaga gaggcaatat ccgcgttaat
    13141 gccgcaatga ccagcaaagg ccatttccgc cagcgttacc agcaggccgc catcagagcg
    13201 gtcgtggtat gcgagcagct tacgctgtgc aaccagcgcc tgaatcgcgt catagaagcc
    13261 tttcagttgc gcgacgtcgc gtacatctgc cggtttgtcg ccaagctgac gataaacctg
    13321 cgccagcgcc gttgcgccca gcgcgttatt gcctttgccc aaatcaatca gcaatagtgc
    13381 gttatcttcg gtagaaagct gtggcgtgat ggtgtgacgc acatcttcca cgcgggcaaa
    13441 tgcagaaatc accagcgaca gcggcgacgt catttcgcgc tcttcgttac cttcctgcca
    13501 gcgggttttc atcgacatag agtctttacc caccgggatc gtcaggccca gcgccggaca
    13561 aagctcttcg cccacggctt taacggcttc atacaggccc gcatcttcac cagggtggcc
    13621 tgccgccgcc atccagttgg cggaaagttt aatgcgtttg atatcgccaa tctgtgttgc
    13681 ggcaatattg gttaacgctt caccgaccgc cagacgggca gaggcggcga aatccagcag
    13741 cgcaaccggc gcacgctcgc caatcgccat cgcttcaccg tagtagctgt cgaggctggc
    13801 ggtagtgacc gcgcagttag cgaccggcac ctgccacgga cccaccatct gatcgcgcgc
    13861 taccatgcca gttacgctgc ggtcaccgat ggtcaccagg aaagttttct ccgccacggt
    13921 cggcagatgc agtatgcgtt tcaccgcgtc tgcaatggtg atcccttcac ggaccagcgc
    13981 atcgcccttc gctttcagcg tttgtacatc gcgggtcatc ttcggcgttt taccaagcag
    14041 gacgtccagc ggcagatcga tcggctgatt atcaaaatga cgatcgtgta gagaaagatg
    14101 cagttcttcg gtcgcttcac caatcaccgc gtagggtgcg cgctcacgct tacacagttc
    14161 gtcaaacagc ggtaattgat cggcagcaac cgccagcacg tagcgttcct gggattcgtt
    14221 acaccagatt tccagcgggc tcatgcctgg ctcgtcgctc agaatgtcgc gcagttcaaa
    14281 tttaccgcca cgcccgccgt cgctcaccag ttccggcata gcgttagaaa gaccgcctgc
    14341 gccaacgtcg tggataaaca gtattgggtt ggcatcacca agctgccagc aacggtcaat
    14401 cacttcctgg cagcgacgct ccatctccgg gttgtcgcgc tgtacggaag caaagtcgag
    14461 gtcggcatca gactgaccag acgccatgga agacgccgcg ccgccgccaa gaccgatgtt
    14521 cattgccggg ccgccgagaa cgaccagctt cgcgccaacg ttgatctcgc ctttttgtac
    14581 gtgatcggcg cgaatgttgc cgatcccgcc cgccagcatg atcggtttgt gataaccgcg
    14641 cagctcttcg ccgttgtggc tgttcacttt ttcttcataa gtacggaagt agccgttcag
    14701 tgccggacga ccaaattcgt tgttaaacgc cgcaccgccc agcgggcctt cggtcatgat
    14761 gtccagcgcg gtgacaatgc gctcaggctt accgaaatct tcttcccacg gctgttcgaa
    14821 gccaagaatt cgcaggttgg aaacggagaa accaaccaaa cccgctttcg gcttcgcgcc
    14881 gcgcccggtg gcaccttcat cgcggatttc accgccggaa ccggtcgccg cgcccggcca
    14941 cggagaaatc gccgtcgggt ggttgtgagt ttcgactttc atcagaatat gcgccggttc
    15001 ctgatggaaa tcatagcggc ccgtttcgtg gtcagcaaag tagcggccca cttcagaacc
    15061 ttccattacg gcggcgttat ctttataagc agagagaacg tgatctggcg tggtttcgaa
    15121 agtatttttg atcatcctga acagcgattt cggctgttgt tcaccgtcga taatccagtc
    15181 ggcgttaaaa attttgtggc ggcagtgctc agagttcgcc tgagcgaaca tatacagctc
    15241 gatgtcgttc gggttacgac caagctttgt gaaagcatcc tgcagatagt caatttcatc
    15301 ttccgccaga gccaggccaa gacgcaggtt agcgtcgatc agcgcctgac ggccctgccc
    15361 cagcaaatca acgcttgtga ccggagtcgg ttgatggtgg gcaaacaact gctctgcatc
    15421 atctaaatca aaaaagacag tttccatcat gcggtcgtgc agttcagcgg taacctgctg
    15481 ccattgttca ttggtcagcg taccggcttc tatatagtaa gcaacgccgc gctcaaggcg
    15541 gtttacctgt tgtagcccgc agttatgggc aatatcggtc gctttcgaag accagggaga
    15601 gatggtgcca ggacgcgggg tcaccagcag gagtttgcct tgcggggcgt ggctggcgag
    15661 tgccggacca tatttcagca ggcgttcaag ttgtgcgtgc tcatcatcgt ttaacggcgc
    15721 attgaggtca gcaaaatgga catactcggc gtaaatattg tgaaccggga gcctggcagc
    15781 ctgaaaacgt gccagcagtt tgttgattcg gaatgccgac agtgcaggcg aaccacgcag
    15841 aatttccatc ataagtctct cgtcttctaa gctttcggtg tacccaaggg ggggggaaac
    15901 gggcgtcatt ataaagaatc tgatgcgctg acgaaaccgt ttgcgtggaa ataaaatcac
    15961 catcgtgaat tagcaacgcg tgccgccaat ggctgtaata agttgccatc tggcgcaggt
    16021 ttacgcaaaa tgccgctcat ttatgagtaa acctttcact attattacgt tttttcaagc
    16081 tgggacgcgc acgacacaga gaattaacta attgaaaaaa ttaaagatta attatctgtt
    16141 catcggcatt ctggcactgc tgctcgcggt cgctctctgg ccatccattc cctggtttgg
    16201 taaagccgac aaccgtatcg ccgccattca agcgcgggga gagttgcgtg tgagcaccat
    16261 tcatactccc ctgacttata acgaaatcaa cgggaaacct tttggcctgg attacgaact
    16321 ggcgaaacag tttgccgatt acctcggcgt aaaactgaaa gtgaccgtgc ggcagaatat
    16381 cagccagctg tttgacgacc ttgataatgg taacgccgac ctgctggcgg caggactggt
    16441 ctataacagt gagcgggtaa aaaattatca gcctggccct acctattatt ccgtgtcaca
    16501 acaactggtt tataaagtgg gtcagtatcg cccacgtacg ctgtgcaacc tgacggcgga
    16561 gcaactcacc gttgcaccgg gtcatgtggt ggttaacgat ctccagaccc tgaaagaaac
    16621 aaaattcccg gaattaagct ggaaggtaga cgacaaaaaa ggctctgcgg aattaatgga
    16681 agatgtcatc gaaggaaaac tcgattacac cattgctgat tctgtcgcca tcagcctgtt
    16741 tcagcgcgtt cacccggagc tcgccgtagc gctcgatatc accgatgaac aaccggtgac
    16801 ttggtttagc ccgttagatg gcgataatac cctttccgcc gccctgctcg acttcttcaa
    16861 cgaaatgaat gaagacggta cgctggcacg cattgaagag aaatacctgg ggcatggcga
    16921 tgattttgat tacgtcgata cgcgcacatt tttacgcgcc gtcgatgcgg tactgccgca
    16981 gttaaagccc ctgtttgaga aatacgccga agaaattgac tggcgtttgc tggccgctat
    17041 tgcttatcag gaatcgcact gggatgcaca ggccacttca ccgacgggtg tgcgcggcat
    17101 gatgatgtta accaaaaata ccgcgcaaag cctcggcatt acggatcgta ccgatgccga
    17161 acagagcatc agcggtggcg tgcgttattt gcaggatatg atgagtaaag tgccggaaag
    17221 tgtgccggag aacgagcgga tctggtttgc cctcgctgcg tacaatatgg gctatgcgca
    17281 tatgctggat gcccgcgccc tgacggcaaa aaccaaaggg aatcctgaca gttgggctga
    17341 cgtaaaacag cgtctgcctt tacttagcca gaaaccctat tacagcaagc tgacttacgg
    17401 ctacgctcgt ggacatgaag cctacgctta tgtcgaaaat attcgtaagt atcaaattag
    17461 cctggtgggt tatctgcaag agaaagagaa gcaggctaca gaagcggcga tgcaactggc
    17521 gcaggattat ccggcggtat cgcctacgga gttgggcaaa gagaaatttc cttttctctc
    17581 gtttctttcc cagtcgtcat caaactattt gacccattct ccctctctgc tgttttccag
    17641 gaaagggagt gaagagaaac aaaattaatc cgtcgaggat tgcgcttttt tctgcgcttt
    17701 aatttcctgg cggcgcatgc gaaagaagtc actgagcaac gccgcgcact catccgccag
    17761 tattccttcc gtaatttcca ctcggtgatt catacccgga tgatgcagca catccattaa
    17821 agatcccgca gcgccagttt tcgcgtcacg cgcaccaaag accacgcgac caatgcgact
    17881 gtggatcatc gctccggcac acattacaca tggttcaagc gtgacataca acgtggcgtc
    17941 gatcagacga taattttgca tcaccagacc accctgccgc agggccatga tttctgcatg
    18001 tgcggtggga tcatggcgac caatcgggcg gttccaacct tcgccgatta cccgattgtt
    18061 atgcactaat accgcgccga ccggcacttc ccgctcatcc caggcacgtt tcgccagcgt
    18121 cagcgcgtga cgcatccagt attcgtggct aaattcgact tcagacaaaa agaaaactcc
    18181 ggttataaaa gcgcggcgca ttatacacgg acgctatgct ttactctatt ccagttgctg
    18241 gagttcaccg cgcggggtta ctcgccaacg atgctgacag aaataaagca gcggattgtc
    18301 ctgattactg tcgctatagc cactgtacag ccgcagcgga gtgccgattt tgcgctccag
    18361 ttgtgcgacc ttttcatgtc ccagacaacg catcgtcaat acccaaccac catagccacg
    18421 ctgaatttgg ctggcgataa gattaacccg cggcagccag ggcttatcga aataaaccgc
    18481 ttcaaccagc ggctgcggag agccggtaat caaccagata tcagcatcgg aacttaacag
    18541 gtaggtggtt aatcgctcct gaaccagcgg aaaggcggta acattgtcgc gaaaccagcg
    18601 cacgaaatcg gcctgcaacg tctgtaaacg tgcttcgctg tgaccaaaag tgcaccccca
    18661 cagaagcaga ctcatcggcc agcgtgccgc acgacctttt atcaataacg caatggctat
    18721 aatcggtaac aacggcagga caagtaacgc attcagcggt tggcgacgta gtaaatagcg
    18781 cagaaaactg ccgaacatat cctgctgatg caatgttcca tctaagtcaa aaaacaccac
    18841 acgacgctcg tgagttgcca aaccatactc ctctggactg aaacatcctg attaattttc
    18901 gctactacat agcctaacag atagatcatc acttttccgg caacaatcga ccgcatcatg
    18961 gctaactgga atttttaatt catggcaatt agcggcaatg gaatataaaa ttcactcgcg
    19021 tgtgtctcat attgcctgtt gtcgccacta tttcatcgcg aggaaagata tgaacggctt
    19081 acttcgtatc cgtcagcgtt accaggggct tgcccagagt gataaaaaac tggcggatta
    19141 tctgctgcta caacctgata cggcgcgcca tttaagctct cagcaactgg ccaacgaagc
    19201 cggagtcagt cagtccagcg tcgtgaagtt cgcgcaaaaa ctcggctata aaggttttcc
    19261 ggcgcttaag ttggcgttga gtgaagcgct ggcaagccag ccggaatcgc cctccgtgcc
    19321 cattcataac caaatccgcg gtgatgatcc gttacggctg gtcggcgaaa aactgattaa
    19381 agaaaatact gccgctatgt acgcaacgct aaacgttaat agtgaagaga aactgcatga
    19441 atgcgtaaca atgttgcgct ctgcgcggcg gataattctg accggtattg gcgcttcggg
    19501 tctggtggcg caaaactttg cctggaagct gatgaagatt ggcttcaatg ctgccgcagt
    19561 gcgcgatatg catgcgctac tcgcaacagt acaggcgtcg tcccctgacg atctgttatt
    19621 agccatttcc tacaccggtg tacgacgcga gttaaacctg gcggcagatg agatgctgcg
    19681 agtgggcgga aaagtgctgg cgattaccgg ctttactccg aatgccctgc aacagcgtgc
    19741 ttctcattgc ctgtatacca ttgccgaaga acaagcgaca aacagtgctt caatctctgc
    19801 ttgtcacgct cagggaatgt taacggattt gctgttcatt gcgctgattc agcaggatct
    19861 ggaactggca ccagaacgta ttcgtcatag tgaagcgctg gtgaaaaaac tggtctgagt
    19921 aaagaatgcg cgtataatgc ccgcccggtt tgtgttgttt tgagagtttc cttatggcgt
    19981 tgttaatcac taaaaaatgc atcaattgtg atatgtgtga acccgaatgc ccgaatgagg
    20041 cgatttcaat gggagatcat atctacgaga ttaacagcga taagtgtacc gaatgcgtag
    20101 ggcactacga gacaccaacc tgccagaagg tgtgcccgat ccccaatact attgtgaaag
    20161 atccggcgca tgtcgagaca gaagaacagt tgtgggataa atttgtgctg atgcaccacg
    20221 cggataaaat ttaatattct actctggaag tagagtatta attatattac tgggaagcct
    20281 taacgccatt atatttattt aattgatgac attagcataa tcattcacta agttaattta
    20341 tatagtatct gccaagacac ttatttatag ttattaaagg cgcgtccgat tggttcaccg
    20401 gacgcacctt aagtacgttt ccttgtgtta taagaacaga aggatcagct gtaaaacagc
    20461 aatgatgatt ttgatgaccc gtttaatcag gtatcggcaa tcagtcattc gtttttcctt
    20521 aaacaaggaa tgcagccgtt cagattagcc cttacatctc cccaaaactg aacgtgcgag
    20581 ttattgaggg tgcatgctgc actccacacc agagctttga cgacaccact cgtttcaatg
    20641 ggggaattct gtggcatggt gtaaagcaca gcaaaatctt caataacgaa gccaatttta
    20701 atgtacttac gaattggcgg tcacgttaat ctttccatca atattgcttt cttcgtaaag
    20761 gctcgagttt ttatgctaaa gattgcaagt tgcttgtaaa agataagtac actgatccat
    20821 aatcgctgtt gttgagggtg catgctgcac aaaattaaag ttaaaaagta aaacccccgt
    20881 tccttaccag ttcgggggtt ttacttttta aagagaacgg tattatttta actttcaata
    20941 attaccgtgg cacaagcata gtggcgttca tctgccagcg ttacatgcat atttacaacg
    21001 ccgagctttt ccgccagttt taatgcctca ccccatagcc gtagccgtgg tttgccgagc
    21061 tcatcattga atacttcaaa ttgattaaac gccagaccat tgcggatccc ggtgccaaac
    21121 gcttttgctg cggcttcttt cacagcaaaa cgcttcgcca gaaaacgcac cggctggtgg
    21181 tgcgttttcc agattgccca ttcgttatcg cttaatacgc ggcgtgccag gcgctcaccg
    21241 gatcgggcga tcactgcttc tatgcgagcg atctctacga tatccgtgcc taaacctaat
    21301 attgccatta gccacgcgct tccagcatca gacgcttcat ttctgccacc gcatctttca
    21361 gtccggtcat cactgcacgg ccaataatgg catgaccgat attcagttca tgcatctcag
    21421 ggatggcggc aatggctttc acgttgtgat aggtcagacc gtgtccggcg ttaactttca
    21481 gaccgagact agcggcaaag gtcgcggctt tggcgatacg caccagctct tgcgcctgtt
    21541 cggcgtcagt tttggcatca gcatagcaac cggtgtggat ctcgataaac ggtgcgccaa
    21601 cctctgccgc agctttgatc tgctcttcat cggcgtcgat aaagagagaa acctgaatcc
    21661 cggcatctgt cagacgtttg caggcatcgc gcattttttc acgctgccct gcaacgtcca
    21721 ggccgccttc ggttgttact tcctgacgct tttccggtac caggcagcaa aaatgtggct
    21781 tcgtctcaac ggcgatcgcc agcatctctt cggtcactgc catctccaga ttcattcggg
    21841 tatccagcgt ctgccgcagg atgcgcacat cgcggtcagt aatgtggcgg cgatcttcac
    21901 gtaaatgcac ggtaatgccg tccgctcccg cctgctcggc aataaacgcc gcctgcaccg
    21961 gatccgggta agcagtaccg cgtgcgttac gcagcgttgc aatatggtca atgttgacgc
    22021 ctaacagtaa ttcagccatg acaatcctca tcattcataa tgtgttttca ccgttcgctt
    22081 aggcataaac tgccggaaca gttccctgct ctttaatggt ttaccgccaa gatacggctt
    22141 aagcgccatg cgggtaaagc gtttcgcggc gcgtagtgtg tctgcgtcag gaaattcccg
    22201 cgcgtttaac gcttttaact gccttccggt gaacgtttta ttgtcgataa cgacgcttgc
    22261 gataaaccct ttttcttcgc gataacgata cgtcatggtg tcatctaccg gctcgccgct
    22321 acccgcgcaa taggtaaaat tgacgccata gcccagatgc ccgagcagtg ccagttcaaa
    22381 gcggcgcagc gcgggttctg gcgtaccagt ggcacctgca agagactgaa tgcagtgcaa
    22441 ataatcgaaa aagagttcag agaagcgcgt ctcgtattcc agtacgcggg agagaagttc
    22501 gttgatgtac agaccgctgt aaagcgtgat accgcttaat ggcagcgcca gcgagacagc
    22561 ttcagcactg cgcagcgttt tgacttcgcc acgcccgccg aaacgtagca agagaggggt
    22621 gaaaggctgt aatgcacctt tcagagtaga gcgtttagag cgtgcgcctt tggcaaccag
    22681 acgcacgcga cccgattcct ccgtgaagac gtccagcatc aggctggttt cgctccacgg
    22741 gcgactatgc aggacaaatg cgcgctgcca gccttccatc ggagtgactc ttaaagatcg
    22801 tcaacgtaac cgagactgcg cagtgcgcgt tcgtcgtcgg cccaaccgga tttcactttt
    22861 acccacagtt caaggtggac aggcgcttcg aacatttcct gcatgtcttt acgcgcttca
    22921 atcccgatgg ttttgatctt ggcccctttg ttgccaataa ccatcttctt ctgcccttca
    22981 cgctcaacga gaatcaaacc attgatgtca taaccaccgc gttcgttaga gacgaaacgt
    23041 tcgatctcca cggtcacgga gtacggcagt tcagcgccga ggaaacgcat cagtttttcg
    23101 cggatgattt cagacgccat aaaacgctgt gagcgatcgg tgatgtaatc ttccgggaag
    23161 tgatgggtcg cttcaggcag atgcttacgc acgattgctg cgatagtgtc gacattcagc
    23221 ccggtttcag cagagatcgg cacgatatcg aggaagttca tctgacttgc caggaactgt
    23281 aagtgcggca gcagatcggc tttttcctgc acgttatcca ctttgttcac cgcgaggatc
    23341 accggcgctt tgccatcgcg cagtttgttg agcaccattt cgtcgtccgg cgtccagcgg
    23401 gtgccttcaa cgacaaaaat caccagctca acgtcgccaa tagagctgct cgccgctttg
    23461 ttcatcaggc ggttaatagc gcgtttttct tccatatgca ggccaggggt atcaacgtag
    23521 atcgcctgat acgcgccttc agtatggatc cccacaatgc ggtgacgagt tgtttgcgct
    23581 ttgcgggagg tgatggagat cttctgcccc agcagtttgt tcaacaatgt ggatttgcca
    23641 acgttcggac gtccgacgat ggcaataaat ccgcagtaac ttttatcgat gctcattcca
    23701 gctccagttt tttcaacgcc tgttcggcgg cagcctgctc agccttacga cggcttgaac
    23761 ctgtgccaac caccggttca ctcaggccgc tgacctggca gtggatagta aattcctgat
    23821 cgtgcgcttc gccacgtacc tggactacca gataagtcgg cagcggcaga tggcgaccct
    23881 gcaaatattc ttgcaagcgc gttttcggat ctttttgttt atcgcctggg ctaatttcgt
    23941 ccaaacgagt ttgataccag ttgaggatta atttctcgac ggtttgaata tcactgtcga
    24001 ggaatacgcc accaattaat gcttcgacgg tatcggcgag aattgactca cgacgaaatc
    24061 caccgctttt aagttcacca ggccctaaac gtaagcactc gcctaactca aattcgcgcg
    24121 ccagttccgc cagcgtattg ccacggacca gcgtggcgcg catccggctc atatcgcctt
    24181 catccacacg agggaaacgg tgataaagcg cattggcgat aacgtagctc agaatagagt
    24241 cgcctaaaaa ttctaaacgc tcgttatgtt tgctgctggc actacgatga gttaatgcct
    24301 gctgcaacag ttcctgatga ttaaaagtgt agcccagctt ccgttgaagc cgattaatta
    24361 cgatggggtt catgcgatac caataaataa atgcgtcaac aattcagcac acgaaacaga
    24421 cctgatatac atggctctgc taactgcttc gctgcagttt cagtttaccg gtatatgggg
    24481 accaacgctg tttcgtgtgc cgtggcaacc tggaggtgcc aaccttaaac ttcgggggaa
    24541 tattctatac acaacgacgg gggatgtcgt tagccacggg agatttatct cataaataat
    24601 tcacgttgtc gccataacgg cgacaacgtg aacgaagatg ggctattaat ggatgccgcc
    24661 aatgcgactt aagcgcacac ctgtcggcca ttcgccttct tgcttatcga aactcatcca
    24721 gatagccgtt gcccgaccga ccagattcgc ttcaggcaca aaaccccagt aacggctgtc
    24781 cgcgctgttg tcgcggttgt cgcccatcat gaagtattgt cccggcggaa caatccaggt
    24841 tgccagttgt tgccctggct gttggtaata catccccacc tggtcctgcg caatcggcac
    24901 tgtcagaatt cggtgcgtca catcacccag tgtctcttta cgctcggaaa gacgaattcc
    24961 attttctttg gtttcgtttt tcggcacttc aaagaatccg ctggtcgctt ccccaccatt
    25021 acggcgtgag aaggtctgaa cgaaatcgct cggttccacg ttggagtagg tgaccggtag
    25081 cgcgttttca cacgcctggc cggaactgca tcccggttga atcgtcagct cttttgagac
    25141 cggatcgtaa gtgactttat cgcccggtaa acccaccgcg cgcttgatgt aatcaagctt
    25201 tggatcttcc ggatatttaa agaccacgat atcgccgcgt ttcggatgac cggtttcgat
    25261 cagcgttttc tggtagatag gatctttaat gccataagca aacttctcta ccagaataaa
    25321 atcaccgatt aacagagtcg gcatcatcga acctgacggg atctggaacg gttcataaat
    25381 aaacgaacgt acaatcaata cgatagccag caccggaaaa acagaagcac cggtttccag
    25441 ccagccaggc ttcggcgcaa cctttttcag ggttgcttta tccagtgagt caccggcagc
    25501 cgcctgcgcc gctgcctgac gttcccgccg tttaggtgcg aaaaagaatt tatccacgca
    25561 ccataaaatg cccgtcacca gtgtggcaat caccagaatc agggcaaaca tattcgccat
    25621 gccaactcct aagggttatt tgttgtcttt gccgacgtgc agaatggcga ggaacgcttc
    25681 ctgcggcagc tcgacgttac cgatctgctt catgcgtttc ttaccttctt tctgcttctg
    25741 cagcagcttt ttcttacggc tgatatcgcc gccataacat ttagccagta cgtttttacg
    25801 cagctgtttc acggtggatc gcgcaataat gtgcgtacca atcgctgcct gaatggcgat
    25861 atcaaactgc tggcgtggga tcagatcttt catcttctcc accaactcgc gaccgcggtt
    25921 ttgcgaatta tcacggtggg tgatcagcgc caacgcatca acacgttcac cgttgattaa
    25981 tacgtctaca cgtaccatgt cggacgcctg gaagcgtttg aagttgtaat ccagagacgc
    26041 ataaccacgc gaggtagatt tcaggcgatc gaagaagtcg agcaccactt ccgccatcgg
    26101 gatctcgtac gtcagcgcca cctgattacc gtggtaaacc atattggtct gcacgccgcg
    26161 tttttctacg cacagcgtaa taacgttgcc gagatatgcc tgcggcagca gcatgtgaca
    26221 ctctgcaatc ggctcgcgca gttcgtaaat gttatttacc gcaggcagct tggatgggct
    26281 gtcgacgtag ataacttccc ttgacgtggt ttcaacttca tacactacgg tcggcgcagt
    26341 ggtgatcaga tccagatcgt attcacgttc cagacgttcc tggatgatct ccatgtgcag
    26401 caggccaagg aagccgcagc ggaaaccaaa gcccagcgcg ctggagcttt ccggctcata
    26461 gaacagtgag gcatcattca ggctgagttt acccagcgcg tcacggaagg cttcatagtc
    26521 gtcggaactt accgggaaca gaccggcgta tacctgcggt ttgactttct taaagccagg
    26581 cagcgccttt tctgccggat tacgcgccag cgttaaggta tcgccgactg gagcgccgtg
    26641 gatatcttta atcgcacata ccagccagcc tacttcgcca catttcagtt cagtgcggtc
    26701 aacctgtttc ggcgtgaaga tgcccagacg gtcggcgtta taggtctgcc cggtactcat
    26761 gactttcact ttgtcgccct tacgcagggt gccgttttta atacggataa gtgaaacaac
    26821 gcccaggtag ttgtcgaacc atgagtcgat aattagtgcc tgcaacgggc cttccggatc
    26881 gccttccggc ggcggaatgt cgcgcaccag acgttcgaga acgtcctgca cgccaacgcc
    26941 ggttttcgct gaacagcgca ccgcgtcagt ggcgtcgatg ccgacgatat cttcaatttc
    27001 ttccgccacg cgttcaggat cggctgccgg caggtcaatc ttgttcagta ctggcaccac
    27061 ttcgagatcc atttccatgg cggtgtagca gtttgccagg gtttgcgctt ccacgccctg
    27121 cccggcgtcg accaccagca atgcaccttc acaggcagcc agcgaacggg aaacttcata
    27181 ggagaagtct acgtggcccg gggtgtcgat aaagttaagc tgataggttt cgccgtcaga
    27241 cgctttgtag tccagcgtca cgctttgcgc tttgatggta atgccacgct cacgctcaag
    27301 atccatggaa tcgagaacct gcgcctccat ttcacggtca gacaggccac cgcagatctg
    27361 gataatacgg tcagacagcg tcgatttacc gtggtcaatg tgagctatga tcgaaaagtt
    27421 acgtatattc ttcataaagt atgattattg tgccttaatg cccggttaac caggctttta
    27481 gaagtcgctg ttctgagctt aacgtctgta ttaatagaaa cgccgcattc tacactacaa
    27541 cattgaggcg aggaaatgtt cataccgtat ggattgtggt atctggaaac gtcctcgcat
    27601 ttgttatgca aaatgcaaca aagccagtga aatcactggc tcgcgtcttc cgaagatgtt
    27661 tcaaatcgca caaggccagg cggcaatgcc acgcttaaga tgatcggttg ccattctgcc
    27721 cgggcagcaa acttacgcga gtagccgcgc gcaatcagga acccgccaat accgccgagg
    27781 accgcaccac ataatgctgc gacgtcagaa gcaaagagta gctgaaatag cgaagcgatg
    27841 aggaataatc ccaccagcgg cgacatataa accagtaatg cggagctaag caggctgcct
    27901 tcggcgatcc ccaattccac tttttgcccc ggcactaacg gttcatcaca gggtacgaca
    27961 atggtatgcg tggtttgcgg gccaagttta ttcaacacgc ggctaccgca accggcgcgt
    28021 gaagcgcagc tgctgcatga agctttaaca tcacaactga ccagcgcctg cccgttttgc
    28081 caggagacga cggtagccca ctctttgatc attgcgctgc cccgaactta atattctcgg
    28141 caatgcgttt cgccgtttgc ggcggcagtt caccgacaat ggtgatttcg gcgttatcac
    28201 gtacgcttgt actgacggtt ctgcgtccgg tgcgcaacat ctgatcggtg ctcgatggcg
    28261 tagcgcggtt aacgtttacc gagaagctga ataatccgtc agaatagaga cgtgattcga
    28321 taggcatgtt gtccatcgtc ggtagcggac gtcgactact ggaaacttcg ctaaaaccct
    28381 gtggcaacca ggttggcgtc cagctgaatt tagctttttc acctacagga acagaaagca
    28441 acggcggcaa atttgccttc gccagcgtct gcatactgct gctgatatcc tgattgacgt
    28501 taaaagcaat cacgcgaaat tgttccagcg tttcaccatc gcgatcaagg agatcaaccc
    28561 gcatcggtaa tttcgattcg gtgtccatcc acacgatgta gctgtagcgt gtaccatctc
    28621 gggcaaccac gcgaatgact tcgcaaagac gatcagcaat acgcgtgcgc ccgacggaga
    28681 taaagtcgta gtaaggagaa aggcgtttga aatcggtata gacgagcgat ggcagagaat
    28741 caacaatgta atcgccatta agcgtgaacg gttcaagtcc cggttcaaaa tagctgattt
    28801 catcgccgcg ctgtaccact tcccggcgcg ggccatccat ttgcaacaat tgtgcaagag
    28861 gacggttatc gaggcgtgca tgtcgataac gcagagactc aacaccctgt ttattgatgc
    28921 tgatgaatga cagctcgtaa ttcagtgact gactggccag gttcatctgc tgtaataacg
    28981 ccccggacgc gggagtggcc gaggcgttag cagagaataa caggctacct gtcactaatg
    29041 acatggcaaa ccaaagttgc ttcattactg cgattgcgtt cctaaagttt gaattcctgg
    29101 cacctgtaca gcggcttgct gggtttgcgc ctgctcaaac tgaagctgtt cagagtggag
    29161 tcggcgttgc agttcgtaat cctgcaacat tgcattaatg cgacgacgct gctcctgtac
    29221 ctgctgctgt tgaccattgt ttgcggtcgc ttcagaaggt actcccaggc ttaccgggct
    29281 ggctttaccc atcatcggca gtgtattaaa taccggcgtt tcgggctgct gggacgtttc
    29341 agattgtcca ttatagtgct ggacgccaac gataactgca agcgatacgc atgctgctac
    29401 gcccatttgg gtaagctgtg ccgcccacgg acgtactttc tgccagaatg gcattttctg
    29461 ccattgatgc ggcgcaggct gggcttccgg gatcaatgtc gccggttgac gtactggctc
    29521 ttcttcaatg gcggccatca cgcgtgaaga gatatcgaaa tggagcacct cgggagtatc
    29581 accccgcatt gagtcacgga ttaagtgata gctttcccag gttttctgca tttctgggtt
    29641 atgagccagt tcgttaagca gctcactatc cagcgtttcg ccatccatta aagcggaaag
    29701 ttgttctttc tgcatgccta atacccttat ccagtatccc gctatcgtca acgcctgata
    29761 agcggttgaa ctttgttatc aatagcttcc ctcgctcgga agatacgtga acgcaccgta
    29821 cctaccggac aatccatgat agcggctatc tcttcatagc tcaggccatc cagctcccgc
    29881 aaggttattg ccatgcgcaa atcttccggg agggactcaa tagttcggaa aactatctgt
    29941 ctcagttctt ctgacaacat taagttctca gggttcgaaa tttctttcaa cgcgccgcca
    30001 ctttcgaagt tttcagcttc aatggcatcc acatcactgg aaggtggacg acgcccctga
    30061 gcaaccaggt aatttttcgc tgtatttaca gcaatccgat acagccatgt ataaaaagcg
    30121 ctatctcccc ggaacgaatc cagcgcacga taggctttaa taaaagcttc ttgtaccaca
    30181 tcgggaacat cacccgacgg cacatagcgg gaaaccagac tcgccacttt atgctgatag
    30241 cgcactacca gtaagttaaa ggctttctga tctcccttct ggacccgttc aaccaggacc
    30301 tggtccgtta actgctcgct catccgaggt aaagtctccc caaaccaaat ttccacgcgc
    30361 tatcgaaacg ccactccatt agctgcaatt tgagcaagca aagggttaga gtgtctcgtt
    30421 tttgtaaagt tccgtaacgc atctgttttt gtttgtcatg ctgtagacgg atcattatct
    30481 atcattataa gtctacagaa tctgaacatc gcattatctg tgtagaaatg cccatttaac
    30541 tgcctgaaga gtaacccaac ggccttttta tttcaccacc taatcctcca ccagccagta
    30601 acttctcttt ttctcgccgc cctgcatcag cgtgtttagc aactgtaaca aatattaaaa
    30661 tagcaggtgt ttattcgcac aacatgatgc tatgctgacc aaaccgtgtt tagtaaatta
    30721 aacaaagaaa atgaatactc tccctgaaca ttcatgtgac gtgttgatta ttggtagcgg
    30781 cgcagccgga ctttcactgg cgctacgcct ggctgaccag catcaggtca tcgttctaag
    30841 taaaggcccg gtaacggaag gttcaacatt ttatgcccag ggcggtattg ccgccgtgtt
    30901 tgatgaaact gacagcattg actcgcatgt ggaagacaca ttgattgccg gggctggtat
    30961 ttgcgatcgc catgcagttg aatttgtcgc cagcaatgct cgttcctgtg tgcaatggtt
    31021 aattgaccag ggggttttgt ttgataccca cgttcaaccg aatggcgaag aaagctacca
    31081 tctgacccgt gaaggaggac atagtcaccg tcgtattctt catgccgccg acgccaccgg
    31141 tagagaagta caatccacgc tggtgagcaa ggcgcagaac catccgaata ttcgtgtgct
    31201 ggagcgcagc aacgcagttg atctgattgt ctctgacaaa attggcctgc cgggcacgcg
    31261 acg
//
LOCUS       DAJVUJ010000029        30870 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.029, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000029 DAJVUJ010000000
VERSION     DAJVUJ010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30870
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.029"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            248..631
                     /gene="secE"
                     /locus_tag="OQQ48_001248"
     CDS             248..631
                     /gene="secE"
                     /locus_tag="OQQ48_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCS1952092.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            633..1178
                     /gene="nusG"
                     /locus_tag="OQQ48_001249"
     CDS             633..1178
                     /gene="nusG"
                     /locus_tag="OQQ48_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCS1952093.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1338..1766
                     /gene="rplK"
                     /locus_tag="OQQ48_001250"
     CDS             1338..1766
                     /gene="rplK"
                     /locus_tag="OQQ48_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCS1952094.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGAGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1770..2474
                     /gene="rplA"
                     /locus_tag="OQQ48_001251"
     CDS             1770..2474
                     /gene="rplA"
                     /locus_tag="OQQ48_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCS1952095.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            2887..3384
                     /gene="rplJ"
                     /locus_tag="OQQ48_001252"
     CDS             2887..3384
                     /gene="rplJ"
                     /locus_tag="OQQ48_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCS1952096.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3451..3816
                     /gene="rplL"
                     /locus_tag="OQQ48_001253"
     CDS             3451..3816
                     /gene="rplL"
                     /locus_tag="OQQ48_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCS1952097.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4136..8164
                     /gene="rpoB"
                     /locus_tag="OQQ48_001254"
     CDS             4136..8164
                     /gene="rpoB"
                     /locus_tag="OQQ48_001254"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCS1952098.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8241..12464
                     /gene="rpoC"
                     /locus_tag="OQQ48_001255"
     CDS             8241..12464
                     /gene="rpoC"
                     /locus_tag="OQQ48_001255"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCS1952099.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQML
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            12677..13216
                     /locus_tag="OQQ48_001256"
     CDS             12677..13216
                     /locus_tag="OQQ48_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-shock protein C"
                     /protein_id="HCS1952100.1"
                     /translation="MKQEVEKWRPFGHPDGDIRDLSFLDAHQAVYVQHHEGKEPLEYR
                     FWVTYSLHCFTKDYEHQTNEEKQSLMYHAPKESRPFCQHRYNLARTHLKRTILALPES
                     NVIHAGYGSYAVIEVDLDGGDKAFYFVAFRAFREKKKLRLHVTSAYPISEKQKGKSVK
                     FFTIAYNLLRNKQLPQPSK"
     gene            complement(13626..14759)
                     /gene="thiH"
                     /locus_tag="OQQ48_001257"
     CDS             complement(13626..14759)
                     /gene="thiH"
                     /locus_tag="OQQ48_001257"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HCS1952101.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAVDVERALNASQFTRDDMMA
                     LLSPAASGYLEQLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAKTEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14756..15526)
                     /gene="thiG"
                     /locus_tag="OQQ48_001258"
     CDS             complement(14756..15526)
                     /gene="thiG"
                     /locus_tag="OQQ48_001258"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HCS1952102.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAEMLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(15528..15728)
                     /gene="thiS"
                     /locus_tag="OQQ48_001259"
     CDS             complement(15528..15728)
                     /gene="thiS"
                     /locus_tag="OQQ48_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HCS1952103.1"
                     /translation="MQILFNDQPMQCATGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHILQDGDQILLFQVIAGG"
     gene            complement(15712..16467)
                     /gene="thiF"
                     /locus_tag="OQQ48_001260"
                     /pseudo
     CDS             complement(15712..16467)
                     /gene="thiF"
                     /locus_tag="OQQ48_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
     gene            complement(16460..17095)
                     /gene="thiE"
                     /locus_tag="OQQ48_001261"
     CDS             complement(16460..17095)
                     /gene="thiE"
                     /locus_tag="OQQ48_001261"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HCS1952104.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVISAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(17095..18990)
                     /gene="thiC"
                     /locus_tag="OQQ48_001262"
     CDS             complement(17095..18990)
                     /gene="thiC"
                     /locus_tag="OQQ48_001262"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HCS1952105.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYLTGTHSGVRV
                     PMREIQLSPTLIGGSKEQPQFEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAATQTIEMGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(19071..19171)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(19224..19700)
                     /gene="rsd"
                     /locus_tag="OQQ48_001263"
     CDS             complement(19224..19700)
                     /gene="rsd"
                     /locus_tag="OQQ48_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HCS1952106.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQKVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19795..20568
                     /gene="nudC"
                     /locus_tag="OQQ48_001264"
     CDS             19795..20568
                     /gene="nudC"
                     /locus_tag="OQQ48_001264"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HCS1952107.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVVLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20608..21672
                     /gene="hemE"
                     /locus_tag="OQQ48_001265"
     CDS             20608..21672
                     /gene="hemE"
                     /locus_tag="OQQ48_001265"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HCS1952108.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKVTRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21682..22353
                     /gene="nfi"
                     /locus_tag="OQQ48_001266"
     CDS             21682..22353
                     /gene="nfi"
                     /locus_tag="OQQ48_001266"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HCS1952109.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLMVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22396..22986
                     /locus_tag="OQQ48_001267"
     CDS             22396..22986
                     /locus_tag="OQQ48_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HCS1952110.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVVLSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQVGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            23173..23445
                     /gene="hupA"
                     /locus_tag="OQQ48_001268"
     CDS             23173..23445
                     /gene="hupA"
                     /locus_tag="OQQ48_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HCS1952111.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23458..24153
                     /locus_tag="OQQ48_001269"
     CDS             23458..24153
                     /locus_tag="OQQ48_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HCS1952112.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALLLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(24155..24580)
                     /gene="zraP"
                     /locus_tag="OQQ48_001270"
     CDS             complement(24155..24580)
                     /gene="zraP"
                     /locus_tag="OQQ48_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HCS1952113.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24818..26194
                     /gene="zraS"
                     /locus_tag="OQQ48_001271"
     CDS             24818..26194
                     /gene="zraS"
                     /locus_tag="OQQ48_001271"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HCS1952114.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIVQHGVISVTVSESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            26191..27515
                     /gene="zraR"
                     /locus_tag="OQQ48_001272"
                     /pseudo
     CDS             26191..27515
                     /gene="zraR"
                     /locus_tag="OQQ48_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
     gene            complement(27512..28801)
                     /gene="purD"
                     /locus_tag="OQQ48_001273"
     CDS             complement(27512..28801)
                     /gene="purD"
                     /locus_tag="OQQ48_001273"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCS1952115.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDDVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACESKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLLLEEVAGGKVFHAGTKLADDEQVVTNGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28813..30402)
                     /gene="purH"
                     /locus_tag="OQQ48_001274"
     CDS             complement(28813..30402)
                     /gene="purH"
                     /locus_tag="OQQ48_001274"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCS1952116.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7315 a         7971 c         8420 g         7164 t
ORIGIN      
        1 gcaaaagttc tgagctaatt gccgataaca tttgactcaa tgcgcactaa aagggcatca
       61 tttgatgccc tttttgcacg ctttcgtacc agaacctggc tcatcagtga ttttctttgt
      121 cataatcatt gctgagacag gctctgttga gggcgtataa tccgaaaagc taatacgcgt
      181 ttcgatttgg tttgcctcgc gatcgcgggg tgaaaatgtt tgtagaaaac ttctgacagg
      241 ttggtttatg agtgcgaata ccgaagctca aggaagcggg cgcggcctgg aagcgatgaa
      301 gtgggtcgtt gtggtggcat tgctcttggt ggcgattgtc ggcaactacc tttatcgcga
      361 cattatgctg ccgctgcgtg cgctggccgt agtaattctg attgctgcag cgggtggtgt
      421 cgcgctgtta acgacaaaag gtaaagctac cgttgctttt gcccgtgaag cgcgtaccga
      481 agtccgtaag gtcatttggc cgactcgcca ggaaacattg cacaccacgc tgattgtggc
      541 tgcggttacc gcagtaatgt cactgatcct gtggggactg gatggtattc tggttcgcct
      601 ggtatccttt atcactggcc tgaggttctg agatgtctga agctcctaaa aagcgctggt
      661 acgtcgttca ggcgttttcc ggttttgaag gccgcgtagc aacgtcgctg cgtgagcata
      721 tcaaattaca caacatggaa gatttgtttg gtgaagtcat ggtaccaacc gaagaagtgg
      781 ttgaaatccg tggcggtcag cgtcgcaaaa gcgaacgtaa attcttccct ggctacgtcc
      841 tcgttcagat ggtgatgaac gacgcgagct ggcacctggt gcgcagcgta ccgcgagtga
      901 tgggcttcat cggcggtact tccgatcgtc ctgcgccaat cagcgataaa gaagtcgatg
      961 cgattatgaa ccgcctgcag caggttggtg ataagccgcg tccgaaaacg ctgtttgaac
     1021 cgggtgaaat ggtccgtgtt aatgatggtc cgttcgctga cttcaacggt gttgttgaag
     1081 aagtggatta cgagaaatct cgtctgaaag tgtctgtttc tatcttcggt cgtgcgaccc
     1141 cggtagagct ggacttcagc caggttgaaa aagcctaacc cagcgatcaa aaaagcggcg
     1201 atttaatcgt tgcacaaggc gcgagattgg aatacaattt cgcgcctttt atttttatgg
     1261 gccttgcccg taaaaacgat tttttatatc acggggagcc tctcagaggc gttattaccc
     1321 aacttgagga atttataatg gctaagaaag tacaagccta tgtcaagctg caggttgcag
     1381 ctggtatggc taacccgagt ccgccagtag gtccggctct gggtcagcag ggcgtaaaca
     1441 tcatggaatt ctgcaaagcg ttcaacgcaa aaactgattc catcgaaaaa ggtctgccga
     1501 ttccggtagt aatcaccgtt tacgctgacc gttctttcac tttcgttacc aagaccccgc
     1561 cggcagcagt tctgctgaaa aaagcggctg gtatcaagtc tggtgccggt aagccgaaca
     1621 aagacaaagt gggtaaaatt tcccgcgctc agctgcagga aatcgcgcag accaaagctg
     1681 ccgacatgac tggtgccgac attgaagcga tgactcgctc catcgaaggt actgcacgtt
     1741 ccatgggcct ggtagtggag gactaagaaa tggctaaact gaccaagcgc atgcgtgtta
     1801 tccgcgagaa agttgatgca accaaacagt acgacatcaa cgaagctatc gcactgctga
     1861 aagagctggc gactgctaaa ttcgtagaaa gcgtggacgt agctgttaac ctcggcatcg
     1921 acgctcgtaa atctgaccag aacgtacgtg gtgcaactgt actgccgcac ggtactggcc
     1981 gttccgttcg cgtagccgta tttacccaag gtgcaaacgc tgaagctgct aaagctgcag
     2041 gcgcagaact ggtaggtatg gaagatctgg ctgaccagat caagaaaggc gaaatgaact
     2101 ttgacgttgt tattgcttct ccggatgcaa tgcgcgttgt tggccagctg ggccaggttc
     2161 tgggtccgcg cggcctgatg ccaaacccga aagtgggtac tgtaacaccg aacgttgctg
     2221 aagcggttaa aaacgctaaa gctggccagg ttcgttaccg taacgacaaa aacggcatca
     2281 tccacaccac catcggtaaa gtggactttg acgctgacaa actgaaagaa aacctggaag
     2341 ctctgctggt tgcgctgaaa aaagcaaaac cgactcaggc gaaaggcgtg tacatcaaga
     2401 aagttagcat ctccaccacc atgggtgcag gtgttgcagt tgaccaggct ggcctgagcg
     2461 cttctgtaaa ctaatgcctt tacgtgggcg gtgattttgt ctacaatctt acccccacgt
     2521 ataatgctta atgcagacgt atatccgaga tattcgggtt gtggcaaggc ggcaactgag
     2581 tgagtcgcca agagcatagc taactatgtg actggtgcga atgaaggaag ccaacgccgc
     2641 cacaagttga atagcgacgg atagaaaaga tttgttcgtt ggagcttggc ctatccaggc
     2701 ctccgtcgaa gaccgcagga gtttcgcaag aaacttaatc ccctgcgtag acggtgacag
     2761 aacgctaaga ttattctttt atattctggc ttgtttctgc tcaccgtaat taagacgctc
     2821 tctccgtttg gaggagtgaa gtgagttcca gagattttct ctggcaaaca tccaggagca
     2881 aagctaatgg ctttaaatct tcaagacaaa caagcgattg ttgctgaagt cagcgaagta
     2941 gccaaaggcg cgctgtctgc agtagttgcg gattcccgtg gcgtaactgt agataaaatg
     3001 actgaactgc gtaaagcagg tcgcgaagct ggcgtataca tgcgtgttgt tcgtaacacc
     3061 ctgctgcgcc gtgctgttga aggtactccg ttcgagtgcc tgaaagacgc gtttgttggt
     3121 ccgaccctga ttgcatactc tatggaacac ccgggcgctg ctgctcgtct gttcaaagag
     3181 ttcgcgaaag cgaatgcaaa atttgaggtc aaagccgctg cctttgaagg tgagctgatc
     3241 ccggcgtctc agatcgaccg cctggcaact ctgccgacct acgaagaagc aattgcacgc
     3301 ctgatggcaa ccatgaaaga agcttcggct ggcaaactgg ttcgtactct ggctgctgta
     3361 cgcgatgcga aagaagctgc ttaatcgcag ttatcttttt aacgcattcg cttacgtata
     3421 aacttattct gatattcagg aacaatttaa atgtctatca ctaaagatca aatcattgaa
     3481 gcagttgcag ctatgtctgt aatggacgtt gtagaactga tctctgcaat ggaagaaaaa
     3541 ttcggtgttt ccgctgctgc tgctgtagct gtagctgctg gcccggttga agctgctgaa
     3601 gaaaaaactg aattcgacgt aattctgaaa gctgctggcg ctaacaaagt tgctgttatc
     3661 aaagcagtac gtggcgcaac tggcctgggt ctgaaagaag ctaaagacct ggtagaatct
     3721 gcaccggctg ctctgaaaga aggcgtgagc aaagacgacg cagaagcact gaaaaaagct
     3781 ctggaagaag ctggcgctga agttgaagtt aaataagcca acccttccgg ttgcagcctg
     3841 agaaatcagg ctgatggctg gtgacttttt agtcaccagc ctttttgcgc tgtaaggcgc
     3901 cagtagcgtt tcacactgtt tgactactgc tgtgcctttc aatgcttgtt tctatcgacg
     3961 acttaatata ctgcgacagg acgtccgttc tgtgtaaatc gcaatgaaat ggtttaagcg
     4021 tgatagcaac aggcattgcg gaaagtgttc cattttccgg tcaacaaaat agtgttgcac
     4081 aaactgtccg ctcaatggac agatgggtcg acttgtcagc gagctgagga accctatggt
     4141 ttactcctat accgagaaaa aacgtattcg taaggatttt ggtaaacgtc cacaagttct
     4201 ggatgtacct tatctccttt ctatccagct tgactcgttt cagaaattta tcgagcaaga
     4261 tcctgaaggg cagtatggtc tggaagcagc tttccgttcc gtattcccga ttcagagcta
     4321 cagcggtaat tccgagctgc aatacgtcag ctaccgcctt ggcgaaccgg tgtttgacgt
     4381 ccaggaatgt caaatccgtg gcgtgaccta ttccgcaccg ctgcgcgtta aactgcgtct
     4441 ggtgatctat gagcgcgaag cgccggaagg caccgtaaaa gacattaaag aacaagaagt
     4501 ctacatgggc gaaattccgc tcatgacaga caacggtacc tttgttatca acggtactga
     4561 gcgtgttatc gtttcccagc tgcaccgtag tccgggcgtc ttctttgact ccgacaaagg
     4621 taaaacccac tcttcgggta aagtgctgta taacgcgcgc atcatccctt accgtggttc
     4681 ctggctggac ttcgaattcg atccgaagga caacctgttc gtacgtatcg accgtcgccg
     4741 taaactgcct gcgaccatca ttctgcgcgc cctgaactac accacagagc agatcctcga
     4801 cctgttcttt gaaaaagtta tctttgaaat ccgtgataac aagctgcaga tggaactggt
     4861 gccggaacgc ctgcgtggtg aaaccgcatc ttttgacatc gaagctaacg gtaaagtgta
     4921 cgtagaaaaa ggccgccgta tcactgcgcg ccacattcgc cagctggaaa aagacgacgt
     4981 caaacttatc gaagtcccgg ttgagtacat cgcaggtaaa gtggttgcta aagactatat
     5041 tgatgagtct accggcgagc tgatctgcgc agcgaacatg gagctgagcc tggatctgct
     5101 ggctaagctg agccagtctg gtcacaagcg tatcgaaacg ctgttcacca acgatctgga
     5161 tcacggccca tatatctctg aaaccttacg tgtcgaccca actaacgacc gtctgagcgc
     5221 actggtagaa atctaccgca tgatgcgccc tggcgagccg ccgactcgtg aagcagctga
     5281 aagcctgttc gagaacctgt tcttctccga agaccgttat gacttgtctg cggttggtcg
     5341 tatgaagttc aaccgttctc tgctgcgcga agaaatcgaa ggttccggta tcctgagcaa
     5401 agacgacatc attgatgtta tgaaaaagct catcgatatc cgtaacggta aaggcgaagt
     5461 cgatgatatc gaccacctcg gcaaccgtcg tatccgttcc gttggcgaaa tggcggaaaa
     5521 ccagttccgc gttggcttgg tacgtgtaga gcgtgcggtg aaagagcgtc tgtctctggg
     5581 cgatctggat accctgatgc ctcaggatat gatcaacgcc aagccgattt ccgcagcagt
     5641 gaaagagttc ttcggttcca gccagctgtc tcagtttatg gaccagaaca acccgctgtc
     5701 tgagattacg cacaaacgtc gtatctccgc actcggccca ggcggtctga cccgtgaacg
     5761 tgcaggcttc gaagttcgag acgtacaccc gactcactac ggtcgcgtat gtccaatcga
     5821 aacccctgaa ggtccgaaca tcggtctgat caactctctg tccgtgtacg cacagactaa
     5881 cgaatacggc ttccttgaga ctccgtatcg taaagtgacc gacggtgttg taactgacga
     5941 aattcactac ctgtctgcta tcgaagaagg caactacgtt atcgcccagg cgaactccaa
     6001 cttggatgaa gaaggccact tcgtagaaga cctggtaact tgccgtagca aaggcgaatc
     6061 cagcttgttc agccgcgacc aggttgacta catggacgta tccacccagc aggtggtatc
     6121 cgtcggtgcg tccctgatcc cgttcctgga acacgatgac gccaaccgtg cattgatggg
     6181 tgcgaacatg caacgtcagg ccgttccgac tctgcgcgct gataagccgc tggttggtac
     6241 tggtatggaa cgtgctgttg ccgttgactc cggtgtaact gcggtagcta aacgtggtgg
     6301 tgtcgttcag tacgtggatg cttcccgtat cgttatcaaa gttaacgaag acgagatgta
     6361 tccgggtgaa gcaggtatcg acatctacaa cctgaccaaa tacacccgtt ctaaccagaa
     6421 cacctgtatc aaccagatgc cgtgtgtgtc tctgggtgaa ccggttgaac gtggcgacgt
     6481 gctggcagac ggtccgtcca ccgacctcgg tgaactggcg cttggtcaga acatgcgcgt
     6541 agcgttcatg ccgtggaatg gttacaactt cgaagactcc atcctcgtat ccgagcgtgt
     6601 tgttcaggaa gaccgtttca ccaccatcca cattcaggaa ctggcgtgtg tgtcccgtga
     6661 caccaagctg gggccggaag agatcaccgc tgacatcccg aacgtgggtg aagctgcgct
     6721 ctccaaacta gatgaatccg gtatcgttta cattggtgcg gaagtgaccg gtggcgacat
     6781 tctggttggt aaggtaacgc cgaaaggtga aactcagctg accccagaag aaaaactgct
     6841 gcgtgcgatc ttcggtgaga aagcctctga cgttaaagac tcttctctgc gcgtaccaaa
     6901 cggtgtatcc ggtacggtta tcgacgttca ggtctttact cgcgatggcg tagaaaaaga
     6961 caaacgtgcg ctggaaatcg aagaaatgca gctcaaacag gcgaagaaag acctgtctga
     7021 agaactgcag atcctcgaag cgggtctgtt cagccgtatc cgtgctgtgc tggtagccgg
     7081 tggcgttgaa gctgagaagc tcgacaaact gccgcgcgat cgctggctgg agctgggcct
     7141 gacagacgaa gagaaacaaa atcagctgga acagctggct gagcagtatg acgaactgaa
     7201 acacgagttc gagaagaaac tcgaagcgaa acgccgcaaa atcacccagg gcgacgatct
     7261 ggcaccgggc gtgctgaaga ttgttaaggt atatctggcg gttaaacgcc gtatccagcc
     7321 tggtgacaag atggcaggtc gtcacggtaa caagggtgta atttctaaga tcaacccgat
     7381 cgaagatatg ccttacgatg aaaacggtac gccggtagac atcgtactga acccgctggg
     7441 cgtaccgtct cgtatgaaca tcggtcagat cctcgaaacc cacctgggta tggctgcgaa
     7501 aggtatcggc gacaagatca acgccatgct gaaacagcag caagaagtcg cgaaactgcg
     7561 cgaattcatc cagcgtgcgt acgatctggg cgctgacgtt cgtcagaaag ttgacctgag
     7621 taccttcagc gatgaagaag ttatgcgtct ggctgaaaac ctgcgcaaag gtatgccaat
     7681 cgcaacgccg gtgttcgacg gtgcgaaaga agcagaaatt aaagagctgc tgaaacttgg
     7741 cgacctgccg acttccggtc agatccgcct gtacgatggt cgcactggtg aacagttcga
     7801 gcgtccggta accgttggtt acatgtacat gctgaaactg aaccacctgg tcgacgacaa
     7861 gatgcacgcg cgttccaccg gttcttacag cctggttact cagcagccgc tgggtggtaa
     7921 ggcacagttc ggtggtcagc gtttcgggga gatggaagtg tgggcgctgg aagcatacgg
     7981 cgcagcatac accctgcagg aaatgctcac cgttaagtct gatgacgtga acggtcgtac
     8041 caagatgtat aaaaacatcg tggacggcaa ccatcagatg gagccgggca tgccagaatc
     8101 cttcaacgta ttgttgaaag agattcgttc gctgggtatc aacatcgaac tggaagacga
     8161 gtaattctcg ctcaaacagg tcactgctgt cgggttaaaa cccggcagcg gattgtgcta
     8221 actccgacgg gagcaaatcc gtgaaagatt tattaaagtt tctgaaagcg cagactaaaa
     8281 ccgaagagtt tgatgcgatc aaaattgctc tggcttcgcc agacatgatc cgttcatggt
     8341 ctttcggtga agttaaaaag ccggaaacca tcaactaccg tacgttcaaa ccagaacgtg
     8401 acggcctttt ctgcgcccgt atctttgggc cggtaaaaga ttacgagtgc ctgtgcggta
     8461 agtacaagcg cctgaaacac cgtggcgtca tctgtgagaa gtgcggcgtt gaagtgaccc
     8521 agactaaagt acgccgtgag cgtatgggcc acatcgaact ggcttccccg actgcgcaca
     8581 tctggttcct gaaatcgctg ccgtcccgta tcggtctgct gctcgatatg ccgctgcgcg
     8641 atatcgaacg cgtactgtac tttgaatcct atgtggttat cgaaggcggt atgaccaacc
     8701 tggaacgtca gcagatgctg actgaagagc agtatctgga cgcgctggaa gagttcggtg
     8761 acgaattcga cgcgaagatg ggtgcggaag caatccaggc tctgctgaag agcatggatc
     8821 tggagcaaga gtgcgaacag ctgcgcgaag agctgaacga aaccaactcc gaaaccaagc
     8881 gtaaaaagct gaccaagcgt atcaaactgc tggaagcgtt cgttcagtct ggtaacaaac
     8941 cagagtggat gatcctgacc gttctgccgg tactgccgcc agatctgcgt ccgctggttc
     9001 cgctggatgg tggtcgtttc gcgacttctg acctgaacga tctgtatcgt cgcgtcatta
     9061 accgtaacaa ccgtctgaaa cgtctgctgg atctggctgc gccggacatc atcgtacgta
     9121 acgaaaaacg tatgctgcag gaagcggtag acgccctgct ggataacggt cgtcgcggtc
     9181 gtgcgatcac cggttctaac aagcgtcctc tgaaatcttt ggccgacatg atcaaaggta
     9241 aacagggtcg tttccgtcag aacctgctcg gtaagcgtgt tgactactcc ggtcgttctg
     9301 taatcaccgt aggtccatac ctgcgtctgc atcagtgcgg tctgccgaag aaaatggcgc
     9361 tggagctgtt caaaccgttc atctacggca agctggaact gcgtggtctt gctaccacca
     9421 ttaaagctgc gaagaaaatg gttgagcgcg aagaagctgt cgtttgggat atcctggacg
     9481 aagttatccg cgaacacccg gtactgctga accgtgcacc gactctgcac cgtctgggta
     9541 tccaggcatt tgaaccggta ctgatcgaag gtaaagctat ccagctgcac ccgctggttt
     9601 gtgcggcata taacgccgac ttcgatggtg accagatggc tgttcacgta ccgctgacgc
     9661 tggaagccca gctggaagcg cgtgcgctga tgatgtctac caacaacatc ctgtccccgg
     9721 cgaacggcga accaatcatc gttccgtctc aggacgttgt tctgggtctg tactatatga
     9781 cccgtgactg tgttaacgcc aaaggcgaag gcatggtgct gactggcccg aaagaagcag
     9841 aacgtctgta tcgctctggt ctggcttctc tgcatgcgcg cgttaaagtg cgtatcaccg
     9901 agtatgaaaa agatgcaaac ggtgaattag tagcgaaaac cagcctgaaa gacacgactg
     9961 ttggccgtgc cattctgtgg atgattgtac cgaaaggtct gccttactcc atcgtcaacc
    10021 aggcgctggg taaaaaagca atctccaaaa tgctgaacac ctgctaccgc attctcggtc
    10081 tgaaaccgac cgttattttt gcggaccaga tcatgtacac cggcttcgcc tatgcagcgc
    10141 gttctggtgc atctgttggc atcgatgaca tggtcatccc ggagaagaaa cacgaaatca
    10201 tctccgaggc agaagcagaa gttgctgaaa ttcaggagca gttccagtct ggtctggtaa
    10261 ctgcgggcga acgctacaac aaagttatcg atatctgggc tgcggcgaac gatcgtgtat
    10321 ccaaagcgat gatggataac ctgcaaactg aaaccgtgat taaccgtgac ggtcaggaag
    10381 agaagcaggt ttccttcaac agcatctaca tgatggccga ctccggtgcg cgtggttctg
    10441 cggcacagat tcgtcagctt gctggtatgc gtggtctgat ggcgaagccg gatggctcca
    10501 tcatcgaaac gccaatcacc gcgaacttcc gtgaaggtct gaacgtactc cagtacttca
    10561 tctccaccca cggtgctcgt aaaggtctgg cggataccgc actgaaaact gcaaactccg
    10621 gttacctgac tcgtcgtctg gttgacgtgg cgcaggacct ggtggttacc gaagacgatt
    10681 gtggtaccca tgaaggtatc atgatgactc cggttatcga aggtggtgat gttaaagagc
    10741 cgctgcgcga tcgcgtactg ggtcgtgtaa ctgctgaaga cgttctgaag ccgggtactg
    10801 ctgatatcct cgttccgcgc aacacgctgc tgcacgaaca gtggtgtgac ctgctggaag
    10861 agaactctgt cgacgcggtt aaagtacgtt ctgttgtatc ttgtgacacc gactttggtg
    10921 tatgtgcgca ctgctacggt cgtgacctgg cgcgtggcca catcatcaac aagggtgaag
    10981 caatcggtgt tatcgcggca cagtccatcg gtgaaccggg tacacagctg accatgcgta
    11041 cgttccacat cggtggtgcg gcatctcgtg cggctgctga atccagcatc caggtgaaaa
    11101 acaaaggtag catcaagctc agcaacgtga agtcggttgt gaactccagc ggtaaactgg
    11161 ttatcacttc ccgtaatact gaactgaaac tgatcgacga attcggtcgt accaaagaaa
    11221 gctacaaagt accttacggt gcggtactgg cgaaaggcga tggcgaacag gttgctggcg
    11281 gcgaaaccgt tgcaaactgg gacccgcaca ccatgccggt tatcaccgaa gtaagcggtt
    11341 ttgtacgctt tactgacatg atcgacggtc agaccattac tcgtcagaca gacgaattga
    11401 ccggtctgtc ttcgctggtg gttctggatt ccgcagaacg taccgcaggt ggtaaagatc
    11461 tgcgtccggc actgaaaatc gttgatgctc agggtaacga cgttctgatt ccgggtaccg
    11521 atatgcctgc gcagtacttc ctgccgggta aagcgattgt tcagctggaa gatggcgtac
    11581 agatcagctc tggtgacacc ctggcgcgta ttccgcagga atccggcggt accaaggaca
    11641 tcaccggtgg tctgccgcgc gttgcggacc tgttcgaagc acgtcgtccg aaagagccgg
    11701 caatcctggc tgaaatcagc ggtatcgttt ccttcggtaa agaaaccaaa ggtaaacgtc
    11761 gtcttgttat caccccggta gacggtagcg atccgtacga agagatgatt ccgaaatggc
    11821 gtcagctcaa cgtgttcgaa ggtgaacgtg tagaacgtgg tgacgtaatt tccgacggtc
    11881 cggaagcgcc gcacgacatt ctgcgtctgc gtggtgttca tgctgttact cgttacatcg
    11941 ttaacgaagt acaggacgta taccgtctgc agggcgttaa gattaacgat aaacacatcg
    12001 aagttatcgt tcgtcagatg ctgcgtaaag ctaccatcgt taacgcgggc agctccgact
    12061 tcctggaagg cgaacaggtt gaatactctc gcgtcaagat cgcaaaccgc gaactggaag
    12121 cgaacggcaa agtgggtgca acttactccc gcgatctgct gggtatcacc aaagcgtctc
    12181 tggcaaccga gtccttcatc tccgcggcat cgttccagga gaccactcgc gtgctgaccg
    12241 aagcagccgt tgcgggcaaa cgcgacgaac tgcgcggcct gaaagagaac gttatcgtgg
    12301 gtcgtctgat cccggcaggt accggttacg cgtaccacca ggatcgtatg cgtcgccgtg
    12361 ctgcgggtga agctccggct gcaccgcagg tgactgcaga agacgcatct gccagcctgg
    12421 cagaactgct gaacgcaggt ctgggcggtt ctgataacga gtaatcgtta atccgcaaat
    12481 aacgtaaaaa cccgcttcgg cgggtttttt tatgggggga gtttagggaa agagcatttg
    12541 tcagaatatt taaggaattt ctgaatactc ataatcaatg tagagatgac taatatcctg
    12601 aaactgactg aactaattga gtcaaactcg gcaaggattc gatactattc ctgtgtaact
    12661 ttcttaagga acgagaatga aacaggaagt ggaaaagtgg cgaccttttg gacatccgga
    12721 tggtgatatt cgtgatttat catttcttga tgctcatcag gctgtctacg ttcagcatca
    12781 tgagggcaaa gagcctttag agtatcgctt ttgggttacc tactctcttc actgcttcac
    12841 aaaagattat gaacatcaga cgaacgaaga aaaacaatcg ttaatgtacc acgcgcctaa
    12901 agaatctcgt cccttctgcc agcaccgtta taacttagcg cgcacacact taaaaagaac
    12961 tattttggcg ctgccagaaa gcaacgttat tcatgccggg tatggtagct atgccgtgat
    13021 tgaggtggac ttagacggag gagataaggc attttacttt gttgcgttca gggctttcag
    13081 ggaaaagaaa aaactccgtt tgcatgtaac tagcgcttat cccatttctg aaaaacagaa
    13141 aggtaaatca gtgaaatttt tcaccattgc ctacaactta ttgagaaata agcagcttcc
    13201 tcagccctca aaataacaaa acccacctta aggtgggttt cgccagagaa ttatctctgg
    13261 tattcagaac gccattaccg gactttgcct tgaccttgcg ataatcgcag gttgcgggat
    13321 gtctgaattt cttcagtctg ctgcatcctg gaagatgaga acatgtgttc ttattttcgt
    13381 ctctatcata gttgagtatt tactctctta caatcagatc tctttcattg ctcaacaggc
    13441 gatggcttca gactttgcat tacggaattt ttaagaaagg cagggcgaaa cgaggaagaa
    13501 gcttttcgcc ctggacaaca ttcctgctga cggcactacc ataaaaaaac acaacgcttt
    13561 caacaacctt gcgtttttca tcttccgtag cggctcggaa taaatttacg ttcttacatt
    13621 ccgtctcata gtctttgcga ggcccgtccc agatagctgt cccagtcttt ccataccggc
    13681 tgcaaaccct gagcggttaa cgcggcagca accgcttccg gtctgcgatc gtcgtgcggt
    13741 gagaactgtt ccaactcggg gtgattatcg gcatagccac ctggctgcgt tttcgagaag
    13801 gcgctgacgt tattgatcgc cagcggaata acgcgatcgc gaaaccacgg tgattcccgc
    13861 gtggagagtg acagttcaat ctccggtgca agcagtcgga aggcgcagat ggtttgcact
    13921 aactggcgtt catccataat cgacgcaggc tcaatgccgc cagtacacgg gcgcaggcgc
    13981 ggaaaggaga cagagtaacg gctttgccag taatgctgtt gcagccatag caaatgttcg
    14041 gcaaccatat agcagtcaac gcgccagttg tcggaaaggc caattagcgc gccgaggcct
    14101 atcttatcaa tccccgcacg ccccagccga tccggcgttt ccagccgcca gaagaagtcc
    14161 tgttttttgc ctttcagatg atggcgggca taagtcgcct cgtgatatgt ctcctgataa
    14221 accatcacgc catccagacc aagctgcttt aactcggcgt attccgtctt cgccagcggt
    14281 tgcacttcca tttgtagtga agagaactgt tcacgcaggg cggggagatg acgacgaaag
    14341 taatccatcc ccactttcgc ctgatgttca ccagtgacta atagcagatg ttcaaagccc
    14401 atctcccgta tagcggcgct ttccctggca atatccgctt catccagcgt tttgcgcttg
    14461 atgcgattac tcatggaaaa tccgcagtac gtgcagtcgt tagcgcaaag attggaaaga
    14521 taaagcggga cgtagaaact aactgtgttg ccaaatcgct gacgggtcag acgctgcgcc
    14581 cgttgggcca gttgttccag atagccactg gcggctggcg ataacagcgc catcatatcg
    14641 tcgcgggtga attgcgaggc atttagcgcc cgctctacgt caacagccgt tttgccgttg
    14701 atacgcaggc ggatgtcatc ccagtccagt tgtcgccagc gatcgctgaa ggttttcatg
    14761 ccgatgcctc cagaaatcca gtcagcgggc tggtggcatg agcaaaatga ctgcggctgc
    14821 ccggtccgga ctgacgtgcc agcaggcctg cttctaccgc cagacgaaat gccttcgcca
    14881 tgttgacggg atcgtccgct acggcaatcg ccgtattcac taacaccgcg tcggccccca
    14941 tttccagggc ctgcgctgca tggctgggaa cgccgatgcc agcatcgaca accaccggaa
    15001 ctgtggcctg ctggataata atctccagca tggcacgggt ttccagtccc tgattcgagc
    15061 caatcggcgc gccgagcggc atcaccgctg cacagccgac ttcttccaga cgtttacaca
    15121 ataccggatc ggccccgcag taaggcagca cgacaaatcc ctgttgtacc agcatttcgg
    15181 cggctttcag agtttcgatg ggatcgggca acagccagcg ggcgtcaggg tgaatctcta
    15241 attttaacca gtttgtgcct aacgcttcac gtgccagatg ggcggcgaaa atggcttctt
    15301 ccgctgtttt cgccccggaa gtatttggca gcagggtcac acccgccgcg ataagcggtt
    15361 cgaggatagc gtcgttgtgc tggcgcaagt cgacacgttt catcgccagt gtcaccagct
    15421 ggctgccgga agcgcggatc gcctccacca tcagttgtga agaagcgaat ttccctgtgc
    15481 cggtaaacag atgtgaatca aacgttttgt ccgcaatacg taacatctca accccctgca
    15541 ataacctgaa aaagcaggat ctggtcgcca tcctgcagga tatgttgcgt ccactgctca
    15601 cgcgggacga tttgctgatt aatcgccaga gccgcgcccg cttgtcgttg gtcgagttgc
    15661 tccagtagtt cgtgaacagt ttgcccggtg gcgcactgca tcggttgatc gttaaacagg
    15721 atctgcattg cttcctccgc ataccgggca accactggcg cggcgcaacg ccaggctgcg
    15781 ccactggctc gatttaccgt cgaacagtcg gagttctccc gcaggtgtct ctataccgct
    15841 taataactta atggcttcca gtgcctgcaa agtgcccata accccgacca ccgggccaac
    15901 cacgcccgcc gtgcggcagt tgcgttctgg ctcctggtta tctggccaca ggcagcggta
    15961 acacccctgc tcccagggcg gcgtcagtac catcaactga ccgccaaatc cgaccgcgct
    16021 ggcggtgata agcggcgtgt tgagtaccac gcaggcggca ttaatctcct ggcgagtcgc
    16081 catattgtcg gtacagtcga gcaccacatc ggcccgtgca accgcatctt ttaacgcctc
    16141 acccgttaac cgttgttgta atgctgtcag ttgaatgtcg ggattcaact gtgtcagtcg
    16201 ctgttggctg acctgcgatt tcgggctgtt gatatcttca gtggtaaaga gaatttgtcg
    16261 ttacagattg cttaaatgca catcgtcgtc atctgccagt accagcgtcc cgacgccagc
    16321 gcccgccagg tatagcgcag caggtgtacc cagcccgcca agaccgataa tcagcacctg
    16381 gctgtcgagc agtttttgct gcccgtcaag agcgatatca tcgagcagga tttggcggct
    16441 ataacgcata aaatcacggt cattcatcgc caactcctgc aatttccagc aactgtgccg
    16501 ttgccaaacg ccagtctgcg gcttgagtaa tggcgctgat gacggcgata ctgccgacac
    16561 ccgttgctat caccgcaggc gcgcgtgcca gactgatacc gccaatcgcc acggtgggat
    16621 aatccgccag tcgctcaaca tgccgtgcca gctgttccag cccctgcggt gcagaaggca
    16681 tctgtttggt ttgcgtcggg aacacatgtc ccagcgcgat ataagagggg cgtgctgcca
    16741 gcgcgacgtc gatttccata tcgtcatgtg tcgaaacacc cagccgcagg cctgccgcgc
    16801 ggatggcatt gagatcggtg gcttgcaaat cttcctgccc caaatggacg ccatacgcct
    16861 gatgcttgat cgcgagccgc cagtaatcgt tgataaacaa tcgcgcgtta tagcggcggc
    16921 ccagcgcaat tgccgccacg acatcggctt ccacctcttc atcgcgccga tctttgatgc
    16981 gtagctggag agtacgtacg cctgcatcca acagacgttc gatccactgt acgctgtcca
    17041 ccactgggta cagtcctaaa cgaaaaggta caggaggaaa atcaggctga tacatcacgc
    17101 ttcctcctta cgcaggtaga tttctccgcc tctggcacgg aagttctccg acatatccgc
    17161 cattcccatt tcaatagttt gcgtggcggc gtaatcacgc acttcctggc tgattttcat
    17221 cgagcagaat ttcggcccac acatggagca aaaatgggcg actttacctg actcttgcgg
    17281 cagggtttca tcgtgataag cgcgggcggt aaacgggtcg agggccagat taaactggtc
    17341 ttcccagcga aattcgaagc gggctttcga catggcgtta tcgcgaattt gcgcgcccgg
    17401 atgccctttc gccaggtcag cggcgtgggc ggcaatctta taggtgataa gcccctgctt
    17461 aacatcttct ttattaggca gacccagatg ctcttttggt gttacgtaac agagcatcgc
    17521 gcagccaaac cagccaatca tcgccgcacc aatccccgac gtgaagtggt catagcccgg
    17581 cgcaatatcg gtagttagcg gccccagagt gtaaaacggc gcttcgtggc agtgctctaa
    17641 ctcctcggtc atattgcggc ggatcatctg catcggcacg tggcctgggc cttcaatcat
    17701 cacctgcacg tcatattccc aggcaatttt ggtcagttcg cccagcgtat gcagctcggc
    17761 aaactgcgct tcatcgttgg cgtcctgaat agaaccgggg cgcagaccgt cgcccagcga
    17821 cagcgaaacg tcataagcgg cacagatttc acaaatttcg cggaagtgtt gatagaggaa
    17881 attttcctga tgatgggaga ggcaccattt cgccataatc gaaccgccgc gagagacgat
    17941 accggtcagg cgtttcgcgg tcatcggcac atagcgcaac agtacgcccg catggatagt
    18001 gaagtaatcc acaccttgct cggcctgttc cagcagcgtg tcgcggaacg cttcccaggt
    18061 aagatcttcg gcgatcccgt taaccttctc cagcgcctgg tagatcggca ctgtaccgat
    18121 cggcaccgga ctgttacgca aaatccactc gcgggtttcg tgaatatagc gaccggtgga
    18181 gagatccatc accgtatccg ctccccagcg cgtggaccat accagctttt ccacttcttc
    18241 ttcgatggaa gaggtgaccg ccgagttgcc gatattggcg ttaactttta ccaggaagtt
    18301 acggccaata atcatcggct ccgattccgg atgattaatg ttggccggga taatcgcacg
    18361 tccggcagca acttcatcac ggacaaattc cgcagtgata ttttccggca gacgtgcgcc
    18421 aaagctcatt cccggatgct ggtggcgtaa aacttcgcta cgtatgcgct cgcggcccat
    18481 attctcgcgg atggcgatga attccatttc cggagtgatg atgccctggc gggcgtagtg
    18541 cagttgggtg acacggcgtc ctgctttggc gcgttttggt gttagtacgc cgctaaaacg
    18601 cagttcgtcg aggccatcat ctgccagccg cgctttagtg taatcggaac tgcgcacggt
    18661 aagttcttcg gtatcgccgc gcgcatcgat ccacggctgg cgtagttttg ccagcccttg
    18721 ctgcacgtta atggcgatct gcggatcgcc atatgggccg gaggtgtcgt agaccggaat
    18781 cgcttcgttt tcttcaaact gcggctgttc tttgctgccg ccgattagcg tcgggctaag
    18841 ctggatctca cgcatcggca cgcgaacgcc agagtgtgtg ccagtgaggt aaatgcgttt
    18901 tgagttggga aaggcggtgc cttccagggt gtcgataaaa tgttgggccc gggcgcgttg
    18961 ttcgcggcgg gtcagttttg ttgcagacat agctcattcc aaaaagttaa ggacgtggct
    19021 tgtcagacga cggatgaagc aagagacgat cgccgcaaag gcgatgcgat agcagattaa
    19081 ctcttgttcc cttcgcaggt attagcctga tcaggttccg cggatcccga ataaacggtc
    19141 tcagccagtt aaggcactcc gacaagaaat tagcccccac aaacggggcg ttgaatgtaa
    19201 attacgcgtt aacagcgcag aactcaagca ggatgtttga cgcgggcggc gtcaagcacc
    19261 agcagaatca gcttatcttc cagcacaaag cgcgcttcca gcgcctcgcc gatgtcagat
    19321 aaaacctttt gaaactcaag gtaattatca tgatcgatgg cggtttccag gctggaatcg
    19381 tagtaatcca taatctgttg ggtattggct tcgagttgcg gccaaatctt cgcggcgcgc
    19441 gcgagttgcc cgttgccttc cagcttatga agaatgcgct cataaatact gaaatgtccg
    19501 gcagacaagt aatcgaccag gctctgacaa aaatcatcaa gggctttttc gtttagcctc
    19561 atgtacgatt ctttgccagg cttaatgcca accagattgt agtaagccac gagcagatgc
    19621 ttacgtacat gtagccagcg atcaaccagt ttgttacttc ctctgacgcg ctccgtcagg
    19681 ttatcgagct ggttaagcat gattgactcc gcaagtttgt attcaaaaac tgctcagtga
    19741 gaaatgtaaa aaccatgtta aacatgccag tgatgcaaag gtagtgcaag agctatggat
    19801 cgtataattg aaaaattaga tcacggctgg tgggtcgtca gccatgaaca aaaattatgg
    19861 ttaccgaagg gagaattgcc atatggcgaa gcggcaaatt tcgatcttgt gggtcagcgc
    19921 gcactacaaa tcggcgaatg gcagggggaa cctgtttggt tagtacaaca gcagcggcgt
    19981 cacgatatgg ggtcggtacg tcaggtcatt gatctcgatg ttgtgctgtt tcaactggcc
    20041 ggacgaggcg tacaactggc ggagttttac cgatcgcata aatactgtgg ttactgcggg
    20101 catgaaatgt atccgagcaa aaccgaatgg gcgatgctgt gcagccattg ccgtgagcgt
    20161 tactacccgc aaatcgcccc ctgcattatt gttgccatcc gtcgcgatga ttcgatcctc
    20221 ctcgcccagc atacccgcca tcgtaacggt gtccatacag tacttgccgg attcgtcgaa
    20281 gtgggcgaaa ccctcgagca ggcagtcgcg cgggaagtga tggaagagag cggaattaaa
    20341 gttaaaaact tgcgttacgt gacttctcag ccgtggccgt ttcctcagtc tttaatgacc
    20401 gcgtttatgg cggaatatga cagcggcgac atcgtgatcg acccgaaaga attgctcgag
    20461 gcgaactggt atcgctatga cgatttgccg ttactcccgc cgcccggcac cgtagcgcgc
    20521 cgtctgatag aagatactgt ggcgatgtgt cgggcagagt atgagtgatg atacactgac
    20581 cgcctgacgc actaaggaac agccaaaatg accgaactta aaaacgatcg ttatctgcgg
    20641 gcgctgctgc gccagcccgt tgatgtcact ccagtatgga tgatgcgcca ggcgggtcgc
    20701 tatctaccgg aatataaagt cacgcgcgcc caggcgggcg attttatgtc gctgtgcaaa
    20761 aacgccgagc tggcgtgcga agtgactttg caaccgctgc gtcgctaccc gctggatgcg
    20821 gcgatcctct tttccgatat cctcaccgtg ccggacgcga tggggttagg gctctatttt
    20881 gaagccggag aaggtccgcg ttttacctcg ccagtcacct gcaaagccga cgtcgataag
    20941 ctgccaattc cggacccgga agatgagctg ggttacgtga tgaacgcggt gcgtaccatt
    21001 cgtcgcgaac tgaaaggcga agtgccgctg attggttttt ccggcagccc gtggacgctg
    21061 gcaacctaca tggtggaagg cggcagcagc aaagccttca ccgtgatcaa aaaaatgatg
    21121 tatgccgatc cgcaggcgct gcacgctcta ctcgataaac tggcgaaaag cgtcactttg
    21181 tatctgaatg cgcagattaa agccggtgct caggcagtga tgattttcga cacctggggc
    21241 ggtgtgctta ccgggcgcga ttatcaacag ttctcgctct attacatgca taaaattgtt
    21301 gatggtttac tgcgtgaaaa cgacggtcgc cgcgtaccgg tcacgctgtt taccaaaggc
    21361 ggcggacagt ggctggaagc gatggcagaa accggttgcg atgcgttggg cctcgactgg
    21421 acaacggata tcgccgatgc gcgccgccgt gtgggcaata aagtcgcgtt gcagggtaat
    21481 atggatccgt cgatgctgta cgcgccgcct gcccgcattg aagaagaagt agcgactata
    21541 cttgcaggtt tcggtcacgg cgaaggtcat gtctttaacc ttggtcacgg cattcatcag
    21601 gatgtgccgc cagaacatgc tggcgtgttc gtggaggcag tgcatcgact gtctgaacaa
    21661 tatcaccgct aaggagtgat tatggatctc gcgtcattac gcgctcaaca aatcgaactg
    21721 gcttcttctg tgatccgcga ggatcgactc gataaagatc caccggatct gatcgccgga
    21781 gccgatgtcg ggtttgagca gggcggagaa gtgacgcgag cggcgatggt gctgctgaaa
    21841 tatccctcgc ttgagctggt ggagtataaa gttgcccgca tcgccaccac catgccttac
    21901 attccaggtt ttctttcctt ccgcgaatat cctgcgctgc tggcagcgtg ggagatgctg
    21961 tcgcaaaagc cggatttagt gtttgtcgat ggtcatggga tctcgcatcc tcgccgtctt
    22021 ggcgtcgcca gccattttgg tttgatggtg gatgtgccaa cgatcggcgt ggcgaaaaaa
    22081 cggctctgcg gtaaattcga accgctctcc agcgaaccgg gcgcgctggc cccactgatg
    22141 gataaaggcg agcagctggc ctgggtctgg cgcagcaaag cgcgctgtaa cccgttgttt
    22201 atcgctaccg gccatcgggt cagcgtggac agcgcgctgg cgtgggtaca acgctgcatg
    22261 aaaggctatc gtctgccgga gccaacgcgc tgggcggacg cggtggcctc ggaacgtccg
    22321 gcgttcgtgc gctatacagc aaatcagccc taattcaggt aaactgcgga taatttccgt
    22381 atttgagaac ttaacatgtt acaaaaccca attcatctgc gtctggagcg cctggaaagc
    22441 tggcagcatg tcactttcat ggcttgctta tgcgaacgca tgtaccccaa ttacgccatg
    22501 ttctgccagc aaaccggttt tggtgatggg caaatttacc gtcgtattct cgatctcatc
    22561 tgggaaacgc tgaccgttaa agacgcaaaa gtaaatttcg acagccaact ggagaaattt
    22621 gaagaagcga ttccttcagc cgacgatttc gatctgtacg gcgtttatcc ggcaatcgat
    22681 gcctgcgtgg tgttaagtga actggtccat tcgcgtttga gtggtgaaac gctcgaacac
    22741 gcggtggaag tgagtaagac ctccatcacg accgttgcga tgctggaaat gactcaggtt
    22801 ggtcgcgaaa tgagcgatga agagcttaaa gaaaacccag ctgtagagca agaatgggac
    22861 attcagtggg aaatattccg acttttagct gagtgcgaag aacgcgatat cgagctgata
    22921 aaaggcctta gggcagacct gcgtgaggcg ggtgagagca atattggtat aatttttcag
    22981 caataagacc agaaaacgtg atttaacgcc tgatttgtcg tacttggagt cttccctttc
    23041 gccccccgtc tggtctacat ttggggggcg aaaaaaagtg gctatcggtg cgtgtatgca
    23101 ggagagtgct attctggcat ttccgtcgca ctcgatgctt agcaagcgat aaacacattg
    23161 taaggataac ttatgaacaa gactcaactg attgatgtaa ttgcagagaa agcagaactg
    23221 tccaaaaccc aggctaaagc tgctctggag tccactctgg ctgcaattac tgagtctctg
    23281 aaagaaggcg atgctgtaca actggttggt ttcggtacct tcaaagtgaa ccaccgcgct
    23341 gagcgtactg gccgcaaccc gcagaccggt aaagaaatca aaattgccgc agctaacgta
    23401 ccggcatttg tttctggcaa ggcactgaaa gacgcagtta agtaagattg cgtggcagtg
    23461 aacagtttta acgaaggggt ggtttcaccc cttttgtctt tctggcgtcg atcattgatg
    23521 ctggctggcg ctctgcttct cactgcctgt agtcataact cttcacttcc tccctttacc
    23581 gccagtggat ttgctgaaga ccagggcgcg gtacgcatct ggcgaaaaga cagcggcgat
    23641 aatgtgcatc tgcttgccgt gtttagcccg tggcgcagtg gcgataccac gacgcgagag
    23701 tatcgctggc agggcgataa cctcacgctc atcaatatca atgtttacag caaaccgccg
    23761 gtgaatattc gcgcgcgttt tgacgatcgc ggtgatctga gctttatgca acgtgaatcc
    23821 gatggggaaa agcagcagct ttctaacgac caaatcgatt tataccgtta tcgtgctgat
    23881 cagatccgcc agattagcga tgccttacgt caggggagag tcgtgctgcg ccaggggcgc
    23941 tggcatgcga tggaacagac cgtgaccacc tgcgaagggc aaaccattaa acctgattta
    24001 gattcgcagg cgatagcgca tatcgagcgc cgccagagcc gctcttctgt tgatgtcagc
    24061 gtggcatggc tggaagcgcc cgaaggttcg caattactgt tagtggcaaa ctctgatttc
    24121 tgtcgctggc aacccaacga gaaaacgttc tgatttacca gtggcccata cccatatgac
    24181 cgccaccacc gcagccgccg tagcccatgc ccattccggc tccgcgcgga atacccgctt
    24241 cagccatcgc gatatctcgt ttcacccgta actcatctaa cgactgacgc aaattctcca
    24301 tctctttggc gaccgcgtta attttgctgc tatccggtgg gttcgcggct aacagggcat
    24361 tgtattcata acgcttcgtc accagttgct gttgcagtgc gctgctttga gcgtaaaagt
    24421 cattatggat tttctgccac gctgtctgtt gttcgctggt caaaggcgcg gcattttgct
    24481 gccacatacc gtgtccgccg tgagcaaatg cagatgtcga tcccatcgcc attgctgaaa
    24541 gcgccatcat taccagggca attttcgtgt tccgtttcat ggttaatcct ccagtggttg
    24601 tcttacttcg ggtattgcat cttccgtgcc aacgatgaaa cgctgatatg acgggtaatc
    24661 tggcatgata aacgagtaaa aatgactcgc ctgctgcggg tagcgagtca tttttactca
    24721 ttgaaactta agcctttgtg ttacagcgca gggtaagcgc tgataaaaga tggcatgatt
    24781 tctgctgtca gaaagggatg agcaggcaaa gaagaagatg cgttttatgc aacgttctaa
    24841 agactcctta gctaaatggt taagcgcgat cctccccgtg gtcattgttg ggctggtagg
    24901 gttgtttgcg gtgacggtga ttcgcgatta tgggcgcgag actgccgccg ccagacaaac
    24961 gctgctggaa aaaggcagtg tacttattcg cgctcttgaa tccggctcgc gcgtcggcat
    25021 ggggatgcgc atgcatcatg cgcagcagca ggccttactg gaagaaatgg ccgggcagcc
    25081 tggagtacgt tggtttgcgg tcacggatga acaaggaaca atcgtgatgc atagcaactc
    25141 cggcatggtg ggaaaacagc tttattcccc gcaggaaatg cagcagttac atccgggaga
    25201 tgaagaagcg tggcggcgga tcgatagcgc agacggtgag cctgttctgg aaatttatcg
    25261 ccagtttcaa ccgatgtttg ctgctggaat gcaccggatg cgccatatgc agcagtatgc
    25321 cgcgacacca caagcaattt tcatcgcttt cgacgccagt aatattgtga gtgccgaaga
    25381 tcgtgagcag agaaacaccc tgattatcct cttcgccctg gcgacggtct tgctggcaag
    25441 cgtattgtca ttcttctggt atcgccgcta tctgcgctcg cgccagcttc tacaagatga
    25501 aatgaagcgc aaagagaagc tggtggcact ggggcatctg gcggcaggcg ttgcccacga
    25561 aatccgtaat ccactttcct caattaaagg gctggcgaaa tactttgccg aacgcgcgcc
    25621 agcaggggga gaagcgcatc aactggcgca ggtgatggcg aaagaagccg accgtttaaa
    25681 ccgcgtggta agcgagttgc tggaactggt taagccaacg catctggctt tgcaggcggt
    25741 ggatctcaac acgctgatta accactcatt acagctggta agccaggatg caaacagccg
    25801 ggagatccag ttacgcttta ccgccaacga cacattaccg gaaattcagg ccgatccgga
    25861 caggctgact caggtcctgt tgaatctcta tctcaatgct attcaggcga ttgttcagca
    25921 tggcgtgatt agcgtgacgg tcagcgaaag cggcgcgggc gtgaaaatca gcgttaccga
    25981 cagcggtaag ggaattgcgg cagatcagct tgaagccatc ttcactccgt acttcaccac
    26041 caaagccgaa ggcaccggat tggggctggc ggtcgtgcat aatattgttg aacaacacgg
    26101 tggtacaatt caggtcgcaa gccaggaggg aaaaggctca acgttcaccc tctggcttcc
    26161 ggtcaatatt acgcgtaagg acccacaagg atgacgcacg ataatatcga tattctggtg
    26221 gtggatgatg acattagcca ctgcactatt ttgcaggctt tactgcgcgg ctggggctat
    26281 aacgtcgcgc tggcgaacag cgggcgacag gcgctggagc aggtgcggga acaggttttt
    26341 gatcttgtgc tttgcgatgt gcgaatggcg gagatggacg gcatcgccac gctgaaagag
    26401 atcaaagcgt taaacccggc aattccggtg ctgattatga ctgcgtactc cagcgtcgag
    26461 acggcggtag aggcactgaa aactggggcg ctggattatc tcatcaagcc gctggatttc
    26521 gataacctac aggcgacgct ggaaaaggcg ctcgcgcata cgcacagtat tgatgctgaa
    26581 acacctgcgg tgactgccag ccagttcggt atggtcggta aaagcccggc gatgcaacac
    26641 ctgctcagtg aaatcgccct cgtcgcgcca tcggaagcca cagtgctgat ccacggcgat
    26701 tccggcaccg gtaaagagct ggtcgccagg gcgattcacg ccagtagcgc acgtagcgaa
    26761 aaaccgctgg taacgctcaa ctgtgcggca ctcaacgaat ccttgctgga atctgaattg
    26821 ttcggtcacg aaaaaggggc gtttactgga gccgataaac gccgggaagg gcgctttgtt
    26881 gaggcggacg gcggcacgct gtttctcgat gaaattggcg atatctcgcc gatgatgcag
    26941 gtgcgtctgc tgcgtgcgat tcaggagcgc gaagttcagc gtgtcggtag caaccagact
    27001 atctcggttg atgtccggct gattgcggcg acccatcgcg atcttgccgc agaggtgaat
    27061 gccgggcgtt ttcgccagga tctctactat cgcctgaatg tggtggcgat tgaagtacca
    27121 tcgctgcggc aacggcggga agatatccct ctgctggctg gccattttct gcagcgcttt
    27181 gccgagcgta atcgcaaggc ggtaaaaggt tttacgcccc aggcgatgga tctgttgatt
    27241 cattacgact ggccgggaaa tattcgtgag ctggaaaacg cggtggaacg ggcagtggtg
    27301 ctgctgccgg ggaatatatt tccgaacgcg agctgccgct ggcgattgcc agtacgccga
    27361 tcccgctggg gcaaagtcag gatattcagc cgctggtgga agtggaaaaa gaggtgattc
    27421 tggcggcact ggagaaaacg ggcggcaaca aaactgaagc cgcccgtcag ttagggatca
    27481 cgcgtaaaac gttattggca aaactgtcgc gttagttctg ctcgcgttcg atagcacgcc
    27541 agccgatatc tttccggcag aagcagtcgt cccagtggat atcggtcatt aaggcatagg
    27601 cgcgtttctg cgcttctgcc acggtatgac ccagcgcggt gacgcacagt acgcgcccgc
    27661 cgttggtcac tacctgctcg tcatccgcca gttttgtgcc cgcgtggaac actttgccgc
    27721 ctgccacttc ttccagcagc aggccgtgga tcacgtcacc ggtgcggtaa tcacccggat
    27781 atccacccgc agccatcacc acgccgagag aagcgcgttc atcccactcg gacgttttct
    27841 cgtccagttt gctttcacag gccgccaggc agagctcaac cagatcggac ttcatgcgca
    27901 gcataatcgg ctgggtttcc ggatcgccaa agcggcagtt aaattcaata accttcggat
    27961 tgccctgttt gtcgatcatc aggcccgcgt agagaaaacc ggtgtaggtg ttgccttccg
    28021 ccgccatgcc tttcacggtt ggccagataa tacgttccat ggtgcgctga tgaacgtcat
    28081 cggttactac cggcgcgggg gagtaagcgc ccatcccgcc ggtgtttggt ccggtatctt
    28141 tatcgcctac gcgtttgtga tcctggctgg tagccatcgg cagcacatgc tcgccgtcca
    28201 ccatcacgat aaagctcgct tcttcgccat cgaggaactc ttcgataacg atgcgatgac
    28261 ccgcgtcgcc aaaagcgttg cccgccagca tatcgtgaac agccgcttcc gcttcttcca
    28321 gcgtcatcgc cacgataacg cctttcccgg cagccagacc gtccgcttta atgacgattg
    28381 gcgcgccttt ctcacgcaga tacgccagcg caggttctac ctcggtgaag ttctggtatt
    28441 ccgccgtagg gatcttatgg cgggccagga aatctttggt aaacgctttt gagccttcca
    28501 gttgggccgc acctgcggtt gggccgaaga ttttcagccc ggcggcgcgg aaggtatcga
    28561 ccacgccttt caccagcggc gcttccgggc cgacgatggt cagatcaatc ttttcgtttt
    28621 gtgcgaaatc caacagcgcc gggatatcgg tcacgccaat agcaacgttt tgcagcgcgg
    28681 gttccagcgc agtgcctgca ttacccggag caacaaaaac agtctcaacc agcggcgact
    28741 gggccgcttt ccaggccagc gcgtgctcgc gcccgccgtt accaatcact aatactttca
    28801 tctattgctc cattaatggc ggaagtggcg catgtcggtg aagagcatcg caataccgtg
    28861 ctcgtcggcg gcggcaatca cttcgtcatc acggatggaa ccgccaggct ggattacgca
    28921 ggtcacgccc gcagcggcgg cggcatcaat accgtcgcgg aacgggaaga acgcgtcaga
    28981 agccatcgag gaacctttca cttccaggcc ttcatcggca gctttaatac cggcgatttt
    29041 cgcggagtac acgcggctca tctggcccgc gccaataccg atggtcatat tgtttttggc
    29101 atagacgata gcattggatt tcacgaactt cgccactttc cagcagaaca gcgcatcgcg
    29161 cagttcctgt tcggtcggct gacgttgggt gacgacgcgc agttcttccg caccgaccat
    29221 ccccaggtca cgatcctgaa ccagcagacc gccgttcacg cgtttgaaat caagacccgg
    29281 aacacgctcg ccccactgac cgcaggtcag aacgcgtacg ttctgcttgg cggcggtgat
    29341 tttcagggct tcttcgctgg cggaaggcgc aataatcact tcgacaaact gacgagaaat
    29401 gatggcctgt gcggtttccg catctagctc gcggttaaag gcaatgatgc cgccgaatgc
    29461 ggaggttggg tcggttttgt acgcgcgatc gtaagcatca agaatggaat tgccgatagc
    29521 tacaccgcaa gggttggcgt gcttcacaat cacacatgcc ggctcggcga actctttcac
    29581 gcactccagc gccgcatcgg tatcggcgat gttgttataa gagagggctt taccctgaac
    29641 ctgggttgcg gtagcaacgg aggcttcttt cacattctct tctatataga aggcagcctg
    29701 ctggtggctg ttctcgccgt aacgcatatc ctgcttctta atgaagttca ggttcagcgt
    29761 gcgtgggaag cgaccggcgg cttctttgct ttcaccgtgg tacgccggaa ccatgctgcc
    29821 gaagtagttg gcaatcatgc tgtcgtaggc ggcagtgtgt tcgaaggctt tgatggcgag
    29881 gtcgaaacgg gttgcaagcg tcagcgatcc ttcgttgtca tccatctctt taataatggc
    29941 gtcgtagtcg ctgctcttca ccacgattgc gacatcttta tggttcttag cggcggagcg
    30001 caccatggtc gggccgccga tatcgatgtt ctcaaccgca tcttccagcg agcagccttc
    30061 acgagcaaca gtctgggcga acggatacag gttaacaacc accatatcga taggctggat
    30121 ctgatgttct tccataatgg catcgtcctg gccgcgacgg cccagaatgc caccatgtac
    30181 tttcggatgc agggtcttca cgcgtccatc catcatctcc gggaaaccgg tgtaatcgga
    30241 aacttcggtt accggcagac ctttttctgc taacagacgg gcagtgcccc ctgtagacag
    30301 cagctccaca ccgcgtgcgg aaagtgcctg ggcgaattcg acgataccgg ctttgtcaga
    30361 aacactgagc agagcgcggc ggactggacg acgttgttgc atggtaaatc ccctggattt
    30421 gactattaca gagagcgtta gctgaatttt tcgcgaaaaa ctcagctaac gcccctaacg
    30481 gggcatcctt atttttcgcc cgcattgtaa cgaaaacgtt tgcgcaacgc tcgcgaattt
    30541 ttctctttca atggtgatca caattttgac agtggctacc gtgggaaaaa tacagaaatt
    30601 acattgatga ttgtggataa ctctgtgcgt aaaaaggtat aaagcggggt tttgctgggg
    30661 aatgcagcag tcagtcattt ttctgcaatt tttctattgc ggcctgcgga gaactcccta
    30721 taatgcacct ccatcgacac ggcggatgtg aatcacttca cacaaacagc cggttcggtt
    30781 gaagagaaaa atcctgaaat tcagggttga ctctgaaaga ggaaagcgta atatacgcca
    30841 cactcacgac agtgcgctaa agctcgtcgc
//
LOCUS       DAJVUJ010000030        29989 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.030, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000030 DAJVUJ010000000
VERSION     DAJVUJ010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29989
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.030"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(1..858)
                     /locus_tag="OQQ48_001275"
                     /pseudo
     CDS             complement(1..858)
                     /locus_tag="OQQ48_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310631.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
     gene            complement(833..1633)
                     /gene="otsB"
                     /locus_tag="OQQ48_001276"
                     /pseudo
     CDS             complement(833..1633)
                     /gene="otsB"
                     /locus_tag="OQQ48_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
     gene            complement(1800..2786)
                     /gene="araH"
                     /locus_tag="OQQ48_001277"
     CDS             complement(1800..2786)
                     /gene="araH"
                     /locus_tag="OQQ48_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HCS1952117.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(2801..4315)
                     /gene="araG"
                     /locus_tag="OQQ48_001278"
     CDS             complement(2801..4315)
                     /gene="araG"
                     /locus_tag="OQQ48_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
                     /protein_id="HCS1952118.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(4385..5373)
                     /gene="araF"
                     /locus_tag="OQQ48_001279"
                     /pseudo
     CDS             complement(4385..5373)
                     /gene="araF"
                     /locus_tag="OQQ48_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
     gene            6087..6590
                     /locus_tag="OQQ48_001280"
     CDS             6087..6590
                     /locus_tag="OQQ48_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HCS1952119.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(6669..6920)
                     /locus_tag="OQQ48_001281"
     CDS             complement(6669..6920)
                     /locus_tag="OQQ48_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HCS1952120.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFSNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(7035..7121)
                     /gene="azuC"
                     /locus_tag="OQQ48_001282"
     CDS             complement(7035..7121)
                     /gene="azuC"
                     /locus_tag="OQQ48_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HCS1952121.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            7384..7707
                     /locus_tag="OQQ48_001283"
     CDS             7384..7707
                     /locus_tag="OQQ48_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HCS1952122.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            7878..8375
                     /gene="ftnA"
                     /locus_tag="OQQ48_001284"
     CDS             7878..8375
                     /gene="ftnA"
                     /locus_tag="OQQ48_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HCS1952123.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQHLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(8413..8652)
                     /locus_tag="OQQ48_001285"
     CDS             complement(8413..8652)
                     /locus_tag="OQQ48_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HCS1952124.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSKDGFSTDQSKICRH"
     gene            8843..10054
                     /gene="tyrP"
                     /locus_tag="OQQ48_001286"
     CDS             8843..10054
                     /gene="tyrP"
                     /locus_tag="OQQ48_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HCS1952125.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNVRKLRRVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVR"
     gene            complement(10116..10781)
                     /gene="yecA"
                     /locus_tag="OQQ48_001287"
     CDS             complement(10116..10781)
                     /gene="yecA"
                     /locus_tag="OQQ48_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HCS1952126.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTNHAILDVAELDDLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(10977..11063)
                     /locus_tag="OQQ48_001288"
     tRNA            complement(10977..11063)
                     /locus_tag="OQQ48_001288"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11027..11029),aa:Leu,seq:taa)
     gene            complement(11076..11149)
                     /locus_tag="OQQ48_001289"
     tRNA            complement(11076..11149)
                     /locus_tag="OQQ48_001289"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11115..11117),aa:Cys,seq:gca)
     gene            complement(11204..11279)
                     /locus_tag="OQQ48_001290"
     tRNA            complement(11204..11279)
                     /locus_tag="OQQ48_001290"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11244..11246),aa:Gly,seq:gcc)
     gene            complement(11431..11979)
                     /gene="pgsA"
                     /locus_tag="OQQ48_001291"
     CDS             complement(11431..11979)
                     /gene="pgsA"
                     /locus_tag="OQQ48_001291"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCS1952127.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(12036..13868)
                     /gene="uvrC"
                     /locus_tag="OQQ48_001292"
     CDS             complement(12036..13868)
                     /gene="uvrC"
                     /locus_tag="OQQ48_001292"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCS1952128.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEDEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(13865..14521)
                     /gene="uvrY"
                     /locus_tag="OQQ48_001293"
     CDS             complement(13865..14521)
                     /gene="uvrY"
                     /locus_tag="OQQ48_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HCS1952129.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            14816..14992
                     /locus_tag="OQQ48_001294"
     CDS             14816..14992
                     /locus_tag="OQQ48_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1952130.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            14979..15203
                     /locus_tag="OQQ48_001295"
     CDS             14979..15203
                     /locus_tag="OQQ48_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HCS1952131.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(15270..16018)
                     /gene="sdiA"
                     /locus_tag="OQQ48_001296"
                     /pseudo
     CDS             complement(15270..16018)
                     /gene="sdiA"
                     /locus_tag="OQQ48_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
     gene            complement(16248..17000)
                     /gene="yecC"
                     /locus_tag="OQQ48_001297"
     CDS             complement(16248..17000)
                     /gene="yecC"
                     /locus_tag="OQQ48_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HCS1952132.1"
                     /translation="MSAIEVKNLVKKFQGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     LGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(16997..17665)
                     /gene="tcyL"
                     /locus_tag="OQQ48_001298"
     CDS             complement(16997..17665)
                     /gene="tcyL"
                     /locus_tag="OQQ48_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HCS1952133.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPILSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(17680..18666)
                     /gene="dcyD"
                     /locus_tag="OQQ48_001299"
     CDS             complement(17680..18666)
                     /gene="dcyD"
                     /locus_tag="OQQ48_001299"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HCS1952134.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLNLFNTQIEMCDTLTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(18771..19571)
                     /gene="tcyJ"
                     /locus_tag="OQQ48_001300"
     CDS             complement(18771..19571)
                     /gene="tcyJ"
                     /locus_tag="OQQ48_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCS1952135.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(19659..20210)
                     /gene="fliZ"
                     /locus_tag="OQQ48_001301"
     CDS             complement(19659..20210)
                     /gene="fliZ"
                     /locus_tag="OQQ48_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HCS1952136.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQAHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDENGWQVEQQSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(20256..20975)
                     /gene="fliA"
                     /locus_tag="OQQ48_001302"
     CDS             complement(20256..20975)
                     /gene="fliA"
                     /locus_tag="OQQ48_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HCS1952137.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLSTKLGKL"
     gene            complement(21319..23001)
                     /locus_tag="OQQ48_001303"
     CDS             complement(21319..23001)
                     /locus_tag="OQQ48_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HCS1952138.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRILELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGKGTIANKAATVSDLTAAGATGTGPYAVTTNNTALSASDALS
                     RLKTGDTVTTTGSSAAIYTYDAAKGNFTTQATVADGDVVNFANTLKPAAGTTASGVYT
                     RSTGDVKFDVDANGDVTIGGKAAYLDATGNLSTNNAGIASSAKLSDLFASGSTLATTG
                     SIQLSGTTYNFGAAATSGVTYTKTVSADTVLSTVQSAATANTAVTGATIKYNTGIQSA
                     TASFGGANTNGAGNSNDTYTDADKELTTTASYTINYNVDKDTGTVTVASNGAGATGKF
                     AATVGAQAYVNSTGKLTTETTSAGTATKDPLAALDEAISSIDKFRSSLGAIQNRLDSA
                     VTNLNNTTTNLSEAQSRIQDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQ"
     gene            23267..24673
                     /gene="fliD"
                     /locus_tag="OQQ48_001304"
     CDS             23267..24673
                     /gene="fliD"
                     /locus_tag="OQQ48_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HCS1952139.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGGDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAKNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKKQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            24698..25108
                     /gene="fliS"
                     /locus_tag="OQQ48_001305"
     CDS             24698..25108
                     /gene="fliS"
                     /locus_tag="OQQ48_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HCS1952140.1"
                     /translation="MYAAKGTQAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            25108..25474
                     /gene="fliT"
                     /locus_tag="OQQ48_001306"
                     /pseudo
     CDS             25108..25474
                     /gene="fliT"
                     /locus_tag="OQQ48_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015018.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
     gene            25552..27039
                     /gene="amyA"
                     /locus_tag="OQQ48_001307"
     CDS             25552..27039
                     /gene="amyA"
                     /locus_tag="OQQ48_001307"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCS1952141.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYNLFDLGEFDQKGSIPTKYGDKVQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVITDEHGEATFFCNGGSVSVWVIEEVI"
     gene            complement(27073..27486)
                     /locus_tag="OQQ48_001308"
     CDS             complement(27073..27486)
                     /locus_tag="OQQ48_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCS1952142.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLALMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            27673..28878
                     /gene="yedE"
                     /locus_tag="OQQ48_001309"
     CDS             27673..28878
                     /gene="yedE"
                     /locus_tag="OQQ48_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HCS1952143.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFTALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            28875..29108
                     /gene="yedF"
                     /locus_tag="OQQ48_001310"
     CDS             28875..29108
                     /gene="yedF"
                     /locus_tag="OQQ48_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HCS1952144.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVRDIQQDGPTIRYLIQK"
     gene            29215..29886
                     /locus_tag="OQQ48_001311"
     CDS             29215..29886
                     /locus_tag="OQQ48_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HCS1952145.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPFFIYRADGQPIFMAAIGSTPFEHGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAQEWMRQEIGGKEASEIATNGCVPANQFTWHPVSRAVGNVKNQGAEL
                     IQPVC"
BASE COUNT         7455 a         7704 c         7186 g         7644 t
ORIGIN      
        1 taccatgatg ctgcggatat ttttccagca acgcttcata ggcgagaaaa cgctctggca
       61 aacctttgga ataatccagc cgttcgacag aaaagatatt ttgtacgttt ttcagttccg
      121 ctttaagttg cgccagtttt ggcggcagtg gcccggcagc ctgtttggct atttctttcg
      181 gttcaatgcc gatcgggtag acttctgttc gaaatgcttt gccccaggct gtatggcttt
      241 ttgcgctacg tgtcgtgacg cgggtcaggt tagaaagaca atccaggaac gccagacgat
      301 cgttttctgt ctggaaaccc agcaaatcat aatcacaaag ctgttcaagc aaggtgtcat
      361 atgtcggcag cgcgttgaag atttccggtg tcgggaaagg aatatgcaga aagaaaccaa
      421 tgcgattatt caccccacgt ttgcgtaatt catgcgcaaa gggcaacagg tgataatcgt
      481 ggatccagat aatgtcatcg tcttgcaaca gcggcagtaa tttatctgcc agcaacgcat
      541 ttacgcgtag atagccgtcc caggcaggac gctgaaattg catcagatcg agccgataat
      601 gaaaagcggg ccagagaacg gcattggaga attggttgta gtattcgtca aggtcctgtt
      661 cgctgaggtt aaaagaggcc cacgtaatgt tacctttttt agcggctgat cctcattccc
      721 tgtttcacca ctccagccaa accacagtcc gcctgtggct ttcagtgccc ccagtatgcc
      781 aacggcaagg ccaccggcac tggcggcgtg ctcgtctggt ggtgcaatcc ggttagatac
      841 tacgactaaa cgactcatag tcatcactcc tgttattttc tcttttttgt tgtaatgcgg
      901 tggttatcat ttcaagcctg ctccagacat ccggcacacc cgccagtcgc catgatgcct
      961 gagttgcacc tgtgccaatt tttactgaca ttccgcccag tcggttaacg actgcgaagc
     1021 cagattcatc ggttaaatca tcgcccagaa atacgggcgt tcgcccgata aagggagctt
     1081 cctgcataaa agctgcaatt gcctcacctt tactggtacc tctcggtttg atctcgacaa
     1141 cacactttcc ctgctgtaac gccatttgtg gccagatctg agtaatacgt tacgctaatg
     1201 tcattaatgc gtcttcatgc tgcggagcct gacgataatg cagcgcaaaa gccatccctt
     1261 tcgcctccag ctccgcgccg ggatactgag cgataactgt atgcagttgc acgctaatat
     1321 cacgcgcaat cgcatccggc agatgaacga tatgcgtttt accattgatg tcacggcgct
     1381 ccgccccatg cacgcccgct aacgggaagc gataaggttt tgccagtgcg tcaagctcca
     1441 ccattgagcg ccctgatatc aatgccaatg caccatcact tgcggttgcc agtagctgta
     1501 gtccttgcag aatattgtca ggcacgacga cctgatcggg atgcggtttg atttccgcca
     1561 gcgttccatc gagatcaaaa aaccaggcat atttcgcgga tagttcaggg gtttcggtta
     1621 acggttctgt cacccggttc tcctacttct cttttcttat tgttttgcgc agaacgcgct
     1681 ttacagacat gtaagtatag acagtgtgac gggggtcgcc atttgcaacg aagaacagcc
     1741 aaccgttatg gcggttggct gtggtgggaa aaaacgctaa attgttgcag aaaaaagcat
     1801 cagacagtgc gtttcgcttt ttgcttgtaa cggtcgaaga tcaccgctgc cagcaggatt
     1861 aagccgcgaa ccacgtactg cgcgaaagga gaaatattaa gcaggttcat ggcgttttcc
     1921 acggtgccta aaattaagat acccgccacc acatatgaga tttttccgat gcctcctttc
     1981 agagaaacgc cacctaaaac gcaggcggag ataacaatca gctcgtaacc aatcgacgtc
     2041 attggctgcc cactggtcat acgtgaagcc agaataattc cggctatcgc agataccagg
     2101 ccagagagaa caaagataat aattttggtg cgaacaaccg gtacacccgc cagacgcgca
     2161 gcctcttcgt tcccgccaat cgccagggtg ttacgaccaa aggtggtttt attcagcaac
     2221 aaaccaaaga taatcagaca cgcgacggtg agccagattg gcgcaggcag accgaaccag
     2281 ttagcgtaac caagggcaaa gaagctttca tcttcgatac cgaccgcttt accgtctgaa
     2341 atgatgtacg ccagacctcg aacaatctgc atcgttgcca gtgttgtgat cagagcattt
     2401 attttcagtt tggcgataac aaagccattg accaggccac agagaatgcc aagcagcaac
     2461 cccgctgcca cgccaatcca caggctttca gtcaggttga taaccaccgc cgtggtgaca
     2521 cccgcacagg caattacgga ggcgacagaa aggtcaaagt caccggaagc aaggcagaac
     2581 aacatgccac acgccaccat ccccgacatg gaaattgcca ggcccaaccc tttcatatta
     2641 atgaaggtgg caaaatttgg gacaaaaatg gcacaggcga taaagagcac cgcaaacacc
     2701 accagcatgc cgtactgatc ccagatacgc ccgaagctga atgacgactt aggtgcgcca
     2761 gaacccgatg tagaaacaga agacatcata ctctccttac tcaggcaact gcctggctga
     2821 ctttaggcat cgcaaggctc agtgcctgac gctcatctgc ctgctcgtgt aacaattcac
     2881 cggcgatttc accttcccgc atcaccacaa tccggtcggc aacgccgagg acttcaggta
     2941 agtcgctgga ggcaaacagc accgccacac cctgcgccgc cagcgcataa attacgttat
     3001 atatttcgtg cttagcacca acatcaatgc cgcgcgtagg ttcatccagc aaaatgacct
     3061 tcatctcttc cgataaccag cggcccagaa tggctttttg ctgatttccg cctgagagat
     3121 tcatgatcag ttgctcagcg cccggcgttt tgatgttgag cgaacgaatg tgctgatcgg
     3181 cattgttttc ttcccaaccg ttgttgatta cacaaccgcc gagcacatgt ttgcgtctgg
     3241 cactgatgtt gatattgtcg cgaacggagt gcacgggaat aatgccttcc gctttgcgat
     3301 cttccgggca gagcatcatg cctgcggcaa tggcgtggct cggtttacga atatcgatcg
     3361 gctgttggtc gatataaacc tgaccggcgg tgatttgcgt cccgccaaac aagcctttca
     3421 ttaattcgct acgccccgcc cctaccagac caaacagccc aacaatttca ccactgcgaa
     3481 ccgccagact tattggcgta cgcacgcctg gtgctttcac agcatcaaga cgcaggcgct
     3541 cctcgccata actacgaggt tgccagccgt agatatcgcc aatgtcgcgc ccgaccatcg
     3601 cctgcaccag cgcgtcgtgg tcaacctgct gcatatcggt aaaggttttg acataacgtc
     3661 catctttaaa gacagtgatg gcatcgctga gggcaaatat ttcttccata cggtgagaaa
     3721 cgtataagat cacccgcccc tcttttcgca gttcacgaat aacgcggaaa agattgtcga
     3781 tttcgcgggc agaaagggag ctggttggct catcaaaggc gataatttta gcgttacgcg
     3841 ccagcgcctt ggcgatttca accatctgcc actgaccaat ggagagatat ttcagcggcg
     3901 tgtccgggtc aatatccata ccaagatgtt taagttgtaa acccgcctca taattcagca
     3961 atgagcgatt cacaatgccg cctttatgcg gcagctggcc gagatagatg ttttccgcga
     4021 cggtcatttc cggcacgaga tgcagttcct ggtaaataat cgccacaccc gcgttaagtg
     4081 ctgcggtcgt gtcggaaaag gacatttcct gcccattaat cactacagaa cccgtggttg
     4141 gcgcatagtt gccgctgagg atttttaaga gagttgattt tcctgcgcca ttttcaccca
     4201 tcaacgcatg aacctgaccg gcatagcagt caaaactaat atccgtcagc gccttaacgc
     4261 cgggaaacgt tttaccgatg ccgcgaaatg agagatacgg ggtagactgt tgcataacgt
     4321 ctccgtgaat cactggtcgt tcacaccccc tctgcatcat gcagagggga atttttccgg
     4381 caaattactt accgcctaaa ccttttttct ccagttcttc tttaaagttg tcacgcgtga
     4441 tcagcaccac gtcggtaact tcggtaaatt ttggcggttc aacgtctttt gctacccagt
     4501 tgtaaagcat ttcgctggat ttatagccat gtacgtccgg gcttggcagc agggaaccgt
     4561 agaagccggt tgcctgtact ttagacagtt cgctcaccgc atccacaccg ttaatgccaa
     4621 tgccgataat atcggcagct ttaaagccct gaccttccgt cgcgcgtacg ccgcccagca
     4681 cggtgctgtc gttcataccg acgatcagcc aatgtttaac ttcaggatgt tgaaccagca
     4741 ttgagttggc agcgtcaaat gcccccggga tgtcgttaga tttggtaggt acctgataaa
     4801 tttgtttttc cgggaatccg gccgctttca gcgcatccat agatcccgta gtacggcggc
     4861 gggcagtatc cagttcgttg gcggtaatca ccatcaccgc gctttctttg acatcccagc
     4921 cacgtttctg catctcttta tacagttcct gaccctgacg ttcgccaatt ttagtcgcgc
     4981 catcatcacc agcggaacgg tatccattgg cttacctttg gcgttaacaa actgatcatc
     5041 cacggcaatg actttcatat cgtagccacg cgctttcgcg acgatggcag agccgagttt
     5101 ggggtccgga gtacaaataa cgaaaccttt tgcgccactg gcagccaggc tgtcgatcgc
     5161 gttcaatgtt ttttcgccat ccggcacggc aatcttaata acctcaaacc ctaaatcctt
     5221 cccggcttta tcggcaaact tccattcggt ctggaagcac ggctcttccg gttgcttcac
     5281 cagaaaaccg agcttcaggt tctccgccat agcggattgt gacataacgg ctgccagacc
     5341 aatggctgcc agggctttag taaatttgtg catggttctc tccagcttta gtgtcgtttt
     5401 gtgtagggca aaaacgaatg acattcgtta aattaatcgg aaaacaaagc attacctttt
     5461 aactaaaaga taagtgaccg tgttgacata gttttagcga gaaattaatt ctccatagga
     5521 gagcaatatc acatcgcaga attacagtga gaacgtgcat aaatttagcg ggaaaagaca
     5581 taagggaaag ccaatttgtc agacaaattg tcgaatgcac agcagattaa tccataagat
     5641 tagcttggaa atccttgttg tctttggtac ccatgcggga tgtcttcttt ttaaccagtc
     5701 aataggccgc attacctggc gttgagtttt tgaaatggtg taataaccgc aactcaaaga
     5761 tgtggaaaat gcacgtcatt catttcgcca ttaattatca ctgtgctcat taattaacag
     5821 aacacgtata atgagagcca tctcgcaaaa atgaaaaaac gttttataaa atcatcattt
     5881 catcaatgaa atgtaaaaat atataaactt gatgatttaa gcattttctt atacccgttc
     5941 agacgttatt cttatttcag atcatcgtca gaattgactc cacgatcaca tttcggaccg
     6001 gcagaaagga attattctgc aaacagtaat tatggtgttt tgatttatct tgcacctctc
     6061 cacttctgga tataaggata ttaggtatgg caaccgctgg aatgcttctc aaactcaact
     6121 ctcaaatgaa ccgcgagttt tacgcatcca atctctacct tcacctgagt aactggtgtt
     6181 ctgaacagag tctgaacggc accgccactt tccttcgcgc ccaggcacag agtaatgtga
     6241 cccaaatgat gcgcatgttt aactttatga agagtgtcgg cgctaccccc atcgttaaag
     6301 ccattgatgt tcccggtgaa aaactgaact ctctggaaga actgttccaa aaaacgatgg
     6361 aagaatacga gcaacgttct agtacgttgg cacagttagc cgatgaagcg aaagaactga
     6421 atgatgattc aaccgtcaat ttcctgcgcg atctggaaaa agaacagcag catgatggtc
     6481 tgttgctgca aaccattctt gatgaagtgc gcagtgcgaa acttgctggt atgtgcccag
     6541 tgcagaccga ccaacatgtt ctgaatgtcg tgtcgcatca gctgcattga tcatacatcg
     6601 gcgctaatgc attgcgccga tgaagttttt gagaaaccgc tgcctcatct ttttgaagca
     6661 gcggtttttt aatgggattc gccttgtggg gtaaacttga gttcaatcag cgcgatggct
     6721 ttttggattg cccgcatggt gaccgggtct gcggcggcgg gatggttact aaagtcgata
     6781 ttcttcagct gactggacat tttttcacga acttcaacgg gcgcgattac atcgagaaca
     6841 tccagaattt gtttgataac caactggcaa gcaaccacat cagaaaccag ttcctgatcg
     6901 gcattcagcg gctgggacat cgtaaactcc tgatagcatt ttgaaagccg ttatagtagc
     6961 ggcttcacat cttcagcgat agtcacatcc accgtcatca ggacacaaaa aaacctgccg
     7021 gagcaggttt tttgttatcg gaacatattg cctggcggta cgtctttgaa cgtcttgcaa
     7081 tagttattga acatactttt caggattttg cgcagtttca tcgcggcact ccgaccattt
     7141 gttatacatg tgttattttc tttgtgctta taatatgaca accatcacaa aaatcaatct
     7201 ttatgtgatg caaatcacac aaataccccc ttaatgttat aaaaatgata atcaaaaaac
     7261 agccccccca tttctgacac ctacagatgg caagaaatag cgcctgccag gcgtcttttc
     7321 cggtcattgt cgcagcgctg taacgcgtaa aatagtgctt tcttttactc ttctggctgg
     7381 accatgagac ttctgattct gactctttca ttaataacgc tggcggggtg tacggttact
     7441 cgccaggccc atgtgagcga agttgacgcg gcaacaggta ttgtacggtt ggtttatgac
     7501 caggcttttt tgcagcatgc ccataccgat cgttatgtca gtcgcgggat cgccgatcgc
     7561 gcatgccagc aggaaggcta tacccacgcg atcccctttg gtcagccagt aggcaactgt
     7621 agcctttttg cgggttcctt atgtttgaat acggaattca ctttgtcata tcagtgccat
     7681 cattccgcct tccctgtctt tctctaaata ttttaatcag cgaggggatc ttcgctgatt
     7741 aaaaaaatag cggtaatgcg ttttattccc attcgtattt ttaataattg aagtttatat
     7801 tttacctttt gcaaataata aaataacaaa ttatagtgac gccacagcaa caaatataac
     7861 ctttgtggag cactatcatg ctgaaaccag aaatgattga aaaacttaat gagcagatga
     7921 acctggaact gtactcttca ctgctttatc agcaaatgag cgcctggtgc agctatcata
     7981 ccttcgaagg tgctgccgcg ttcctgcgcc gtcacgccca agaagagatg acgcatatgc
     8041 agcatctgtt tgattacctg actgataccg gcaatttacc gcgtattaat accgttgaat
     8101 ctccgtttgc tgaatattcc tcacttgatg aattattcca ggaaacctat aaacacgaac
     8161 aattaatcac ccagaaaatt aacgaactgg ctcatgctgc aatgaccaat caggactacc
     8221 caacatttaa tttcctgcaa tggtatgttt ctgagcagca tgaagaagag aaactgttca
     8281 aatcgattat tgataaatta agcctggcag gcaaaagcgg cgaaggtctg tattttatcg
     8341 acaaagaact ctctaccctc gacacacaaa actaatactc tccggcggca ggtttactgc
     8401 cgccgtttca aatcagtgac ggcaaatctt actctgatcg gtcgaaaaac cgtctttcga
     8461 aggtataaat ttgccttttg ctgccagaaa cgctaccagc tctcccgctg tcatcccttc
     8521 tgccgagcag gtgtgaaaac gtgcctgttc accaaaacgc gctttaattg ctgcttccag
     8581 actggcatgc gtatattgct cgcctgattc aatcatcata tttaacactt catgaccgtg
     8641 aatagagtcc atcgttcctc ctcaaaaaaa gcctagcgta gcgattgccg cttatgaaga
     8701 ctttgcgcca gagcagaact gaatgctttt tattgtacat ttatatttac accatatgta
     8761 acgtcggttt gacgaagcag ccgttatgcc ttaacctgcg ccgcagatat cactcataaa
     8821 gatcgtcagg acagaagaaa gcgtgaaaaa cagaactctg ggaagtgttt ttatcgtggc
     8881 gggaaccaca attggcgcag gcatgctggc aatgccgctg gctgcggcag gtgttggttt
     8941 tagcgtcacg ttaatcttgt tgattgggct ttgggcgttg atgtgctaca cggcgctatt
     9001 actgctggag gtgtaccagc atgttccggc agataccggt ctgggcacgc tggcaaaacg
     9061 ctatctggga cgctacggtc aatggctgac gggcttcagt atgatgttct taatgtatgc
     9121 tctgactgcg gcatacatca gcggtgccgg tgaattgttg gcctccagca tcagcgactg
     9181 gacaggtatt tcaatgtcgg caaccgctgg cgtgctgttg ttcacttttg ttgccggtgg
     9241 cgtggtttgt gttggaacat cgctggtcga tttatttaac cgttttctgt tcagcgccaa
     9301 aattattttt ctggtggtga tgctggtatt gctgctaccg catattcaca aagtgaatct
     9361 tttaaccctg ccgttgcaac aggggctggc tctgtcagca atcccggtga tttttacctc
     9421 atttggtttt cacggtagcg tgccgagtat tgtcagctat atggatggca acgttcgtaa
     9481 gctacgcagg gtgtttataa ccggtagtgc gatccccctg gtggcatata ttttctggca
     9541 ggtggcaacg cttggcagca ttgattcaac aacctttatg ggattgctgg ctaatcatgc
     9601 tggattaaac gggctgttac aggcgttacg cgaaatggtg gcctctccgc atgttgagct
     9661 ggcagtgcat ttatttgctg atttagccct cgccacgtca tttctcggcg ttgcgttagg
     9721 cttatttgat tatctggctg atttatttca gcgttcaaat accgttggtg gacggttgca
     9781 aactggtgca attacctttc tgccgccgtt ggcgtttgca ctgttttatc cacgaggatt
     9841 tgtgatggcg ctgggttacg ccggtgtggc gctggcggta ctggcattga ttatcccttc
     9901 gctgttgacc tggcaaagca gaaagcacaa tcctcaggcg ggttaccggg tcaaaggtgg
     9961 tcgtccggcg ctggtggtgg tgtttctctg tggtattgct gtgattggcg tgcaattttt
    10021 gattgcggca gggttgttac cagaagtgag gtgatcagat agcctcaaat tccttattgg
    10081 gtgccagaat taacgctgac acccaatttg gcctcttaat gcaggcagca ctgcttaaat
    10141 ttcttaccac taccgcacgg gcaaggatcg ttacgccccg gtttctcttc tgctttgatc
    10201 ggttgctgaa cagctttttc ctgcggatgc gccatccagt acgcatgtag atcaagcgcc
    10261 gccagtcgaa tggcatctac gctctcttca aacgcttctg gcgacatctt ttctacccgc
    10321 tcgaagtttt cctcagtacc gtgcagcgca atcgcctcca gcgctggttt taacgaatcg
    10381 ggcaacgttg accagtcaga aagtgccacg ccccgcatat agccaaagca ccactcctca
    10441 acaatcgtca gctcgctgcc atcaacttct cgcaagccga ataacggctc aaactgctcc
    10501 gggaattcgt tcagacgctc tgcggtatcg gccatatgtt gaaaagccaa gttcataaag
    10561 cgcgtcatct ctttctctga cgcccagcgc ggcacatagt cagccccacc ccacacggca
    10621 accagccact gttccggttc aatctcttgc ggagaactca acaccgccgt caataaatcg
    10681 tccagctccg ccacatcaag gatggcgtgg ttagtgttgt atttggtcag aatatcgtcc
    10741 agccattcca actcactttc gtttaacggt cccgttttca tacgcttttc cttgtggatc
    10801 tcaactcgcc agcacctatc ttacatgccg gtccgtatca gagatacttt ttgagtggct
    10861 ttgccggtga ttaaaaatta aggagggtgt aacgacaagt tgcaggcaca aaaaaaccac
    10921 ccgaaggtgg tttcacgaca ctgcttattg ctttgatttt attcttatct ttcccatggt
    10981 acccggagcg ggacttgaac ccgcacagcg cgaacgccga gggattttaa atcccttgtg
    11041 tctaccgatt ccaccatccg ggctcgggaa gaaagtggag gcgcgttccg gagtcgaacc
    11101 ggactagacg gatttgcaat ccgctacata accgctttgt taacgcgcca aattcttcag
    11161 gcctttcagc cagacatccg cttgacgccg atgtctttta aactggagcg ggaaacgaga
    11221 ctcgaactcg cgaccccgac cttggcaagg tcgtgctcta ccaactgagc tattcccgca
    11281 ttcattaagc aatcagttaa tcacttgatt ttattatcgt ctggcaatca gtgccgccgt
    11341 tcgatgcgtt gcattctact tacctggcgc gatgagtcaa cgatattttt caccactttt
    11401 gatcgtttgc tgaaaattac gccgaaacga tcactgatca agcaaatctg cacgcgcagc
    11461 gctcaaatat tgcaacattg accacagagt cagcaccgca gccacaaaga aaagtgcaat
    11521 accggcgtac tcaacccaaa tgttcggacg ccacagcagc caggccaacg ccaccatctg
    11581 ggcagtggtt ttcactttcc caatccagga gacagccacg ctactgcgtt tacccaactc
    11641 cgccatccat tcgcgtagcg cagaaataat aatttcacgg gcgatcatcg ttgccgccgg
    11701 tagggtcacc caccagctgt gataatgctc ggttaccagc accatggcga tagccacgag
    11761 aactttatct gctacagggt caaggaaagc accaaaccgg gtactctggt tccagcggcg
    11821 tgccagaaaa ccatcgaacc agtcagtcac cgccgcgacg cagaaaatga gcgcggcggc
    11881 aaacggcgac caggtgacag gcagataaaa gaccaataca aagaatggga taaggatgac
    11941 acggaacagt gtaagcaacg tagggatatt aaattgcata atgacgggta actatctgtt
    12001 gtcagtaaga ttacccctat gttgctacag agacatcaat gtttcaacga ccagaagatc
    12061 ttttctgcca gaccttgcga aatacccggc acttttgcaa tttcctcgac gctggcgtta
    12121 cgtaaacctt gcaaaccgcc catatatttc aacaacattt gccgacgttt tggcccgacg
    12181 ccttcaatgg tttccaggga actggtattt ttgaccttcg cccgtttttt acggtgcccg
    12241 ccaatcgcgt gatcatgtga ttcatcgcga atatgctgga taacatgcag cgcgggtgaa
    12301 tctggcggca aactaaatcc ctcatcttcc ggctcaaaga acagcgtttc cagtccagcc
    12361 ttacgatctg ctcctttggc aacgccaagt agcagcggat gatttttatc ccatgagaca
    12421 tccagttcgg cgaagacatt tttcgcctgc gcaagctggc ctttgccgcc gtcgataagg
    12481 atcacatccg ggatcttact gtcgtcaatg gctttaccat aacgccgacg cagcacctga
    12541 ttcatcgccg cataatcatc gcccggcgtg atgcctgtaa tgttatagcg ccgatactcc
    12601 gcacgcagcg ggccgttagc atcaaacacc acacaggaag cgacggtttg ttcgcccatg
    12661 gtatggctga tgtcaaagca ctccatccgc ttcacttccg gcaatttcaa cacgctggca
    12721 agcgcggtca gtcgctggtg aacggtagat tgctgcgaaa gtttgctggt taaggccgtc
    12781 gccgcattgg tgcgcgcgag tttcagataa cgcgccctat cgccgcgagg tttggtttga
    12841 acattaatct tgcgtcccgc cagttctgaa agggaatcgg cgagcagcgt tttatcgcta
    12901 agattaaaat cgagcaggat ctcacccggt aaggtgcgca tctggctgcc ttgtaaatag
    12961 aactgaccta cgaaggtttc taccacctcg ctcagttccg taccgccagg cactttcggg
    13021 aaatagctgc ggctgccgag cactttgccc tgacgaatga acaatacgtg gacacaagcc
    13081 atgcccgcat cgaacgccac accaataacg tcaaggtcgt cgccggtatt ggaaacaaac
    13141 tgtttttcgg tgacgcgtcg caccgcctga atttggtcgc gaatacgtgc cgcttcttca
    13201 aactccagat tctgactggc agtttccata cggctaatga gttgcgtaag cacctgatca
    13261 tctttgccag acaaaaacag gcgcacatac tcgacctgct gagcgtattc ttcttcactc
    13321 accagtcctt caacgcacgg tcccagacag cgccctatct gatattgcag acacggacgc
    13381 gagcgattgc gataaacact attttcgcac tggcgaatgg ggaaaatctt ttgcagtagc
    13441 gccagagttt cacgtacggc atagccattc gggaacgggc cgaaatattc acctttggca
    13501 tgcttcgcac cacgatgcat cgccagacgc gggtgggtat cgccactcag gaagataaaa
    13561 ggataagatt tatcatcgcg tagcaaaacg ttgtaacgcg gctgatagag tttgatgtag
    13621 ttgtgttcca gcaacagcgc ttcggtttct gtatgagtaa ccgttacatc aatttgctga
    13681 atctgggcga ccagcgcttc ggttttgcgc gaagcgaggt tgctacggaa atagctggaa
    13741 agccgttttt tcaggtcttt cgctttgccg acatagataa ccgtaccacc agcatcgtac
    13801 atgcgataaa cgcctggctg gctggttacg gtttttaaaa acgcttttgc gtcaaactga
    13861 tcactcactg acttgataat gtctccgcat tacacagacc atggcgaatt gccaggtgag
    13921 tcagctcaac atcgccatga atgtttagtt tactgaacat acgatagcgg tagctgttca
    13981 ccgttttcgg actgagattg agctgttctg agatctcatt gaccttctgg cccttggtga
    14041 tcatcagcat aatctgcaat tcacgttcag acaaactggc aaatgggctt tctgtttttt
    14101 ctggttcgat ctggcttaac gccatttgtt gagcgatgtc agaagcaatg taacgctgcc
    14161 ctgaatagac agaacgaatc gcactcacga cttcctgcgg agccgcgcct ttgctgaggt
    14221 agcccgcagc accggcctgc atgactttcg ctggtaaagg gttttctgta tgaacggtaa
    14281 gcatgatgat tttgacatca gctgtggaac gcgcgatttt acgcgtcgcc tcaagaccgc
    14341 caatgcccgg catactcatg tccattagca ccacgtcaac ggcatttgtc cggcaccact
    14401 taacggcgtc ttcaccgcac gatgcctcac cgacgacttt tatacccttt atatcttcca
    14461 gaatgcgtcg tatccctgcg cgcaccagtt cgtggtcatc aacaagtaga acgttgatca
    14521 aagaaatatc tccagaaata gggataacgc tactgatagt tagtcattcg tatattagcg
    14581 gtttttattg caactttgaa acgttaaaaa tggtgggtat tcgatttttc tctcgttttt
    14641 gtcaattcga ttgtccacag ttatggtcac gcccgtcacc aacggcttac cggatcgttt
    14701 cacagtaagc cctacgatga aatgtgacaa aaattgactt tattcagcaa aaatgaaaat
    14761 cagcccgctg aatatgtaac attaattaac ccgaacaata cgggcaaaaa cattcatgat
    14821 aaaaatattt attggtcatt atattaacgt attttatagc actgccgata tcacgctcaa
    14881 aaaacaacca ctgctatttt tagcaaagct tatggtatac tccgccgcct taacattttt
    14941 caccgcaaat tttcattgca acatgacgag gaaaataaat gagtacgcct gatttttcta
    15001 ctgccgagaa taatcaagaa ctggcaaatg aagtctcctg cctgaaagcg atgctgacgc
    15061 tgatgctgca ggcgatggga caagctgacg cgggccgcgt gatgttaaag atggaaaaac
    15121 agcttgcgct gatcgaagac gaaacccagg ctgcagtatt ttccaaaacg gttaagcaaa
    15181 ttaaacaagc ctaccgtcag taatataaaa ccggctgaga gcgtgccttt cagccggttt
    15241 ttgcatctgg cacgcagaaa gaaaagcgat caaattaagc cagtagcggc cgcgtaacag
    15301 gccacctggg tcttatttgg tgcattgatt tttttctgca tgtttttctg atggaaattg
    15361 accgtattct cagagattga caaaatcatc gctatctctg ctgatgtctt cccttccgcc
    15421 gtccatttca gaatttcttt ttcgcgcttg ctgaaattca tctctggcgt catcactatt
    15481 tcatcattta aacgcatcag agccatcaga ctttcgcgca ccagtaactg catttttaat
    15541 tgcagttcat cactaagaat gggtatttcg cgcgtgctgc aacgggaaaa ggacaaaaag
    15601 cccagcgccc gatttggcag cattaaatac tgagtgacac cgcggcgtaa accatgtgcg
    15661 tgcgcggctt cccataatgg ctgtgcttcg ctgaataagt catcattcca cattaaatgg
    15721 ccctgactaa agttttcagg gttcagcaca ggatcaattg cgagaaaatt agcacaggat
    15781 caattgcgag aaaatttttt gcctgataat aactaaccca cgactcaggg taattggtgt
    15841 agaaagccac tttaggtcgc gtgaatggta ccgggtggcg aacacataac gaatagtaat
    15901 cgtactccag ctgctgagcc tgaagctcta tttcatggta gacctcttct gcgacctcca
    15961 tcctctgaaa acgcaacagc atcgtgcgac gccaactgaa aaaatccgta tcctgcatag
    16021 taaaccgcaa cgcccctgag agtgagtatc atttataatg ataattcaaa ctatcacata
    16081 aataagattt atatataatt tatattattc aggcaatgaa ttacttttgc aagccatcgc
    16141 attctcttat gttattaatg agttatgctg atttgttaag cagttttatc acgcttgaaa
    16201 tggcgtcccg ccccgacagg tgaatcgtcg gggctgattt tttcttatta ttgcagcaga
    16261 aacttctcga ggaactgtcg ggtgcgaggc tgctggggat cggcaaataa cgcttttgct
    16321 gccccctgct cgactatccg ccccaggtcc ataaaaatcg cccggtccgc aacatcccgg
    16381 gcaaagctca tttcgtgcgt cacaatcacc atcgtgcgct tttcctgcgc cagctggcgg
    16441 attgtgttca ggacttcgcc caccagctct ggatccagcg ctgacgttgg ctcgtcaaac
    16501 aaaatcacct caggacgcat tgccagcgca cgcgcaatcg ctacacgctg ctgttgaccg
    16561 ccagacaaac gacgtggata gctggtttct ttaccggcca gcccaacttt cgccagcagc
    16621 tcgcgagcgc gcgccgtggc ctcttctttc ggttcgcctt tgacgatcac cggcccttca
    16681 ataatgtttt ccagcaccgt acgatgcgga aacaaattaa agttctggaa gacaaacccg
    16741 acgtgctgac gcaactggcg aatcagcgat ttttgctgac ttaatgaacg tgcagtatca
    16801 atagtgatat cgccaacggt gatcgttccc gcttccggtt gttccagcag atttatgctg
    16861 cgtagcaacg tggttttgcc ggaaccactc ggaccgataa ttgccaccac ttcgccaggc
    16921 tttacctcga ggtcgatacc gtgcagcacc gtctgacctt ggaatttttt caccaggttc
    16981 ttaacttcaa tggcactcat ttgggttctc tctcctggcg attaagttga ttctcaaaat
    17041 ggttctgcaa cgtcgataac acagtcgcca tgatccagta gatgagcgaa gccgccaggt
    17101 acatggtgaa cacctccagc gtgcgcgagg taatcaactg cgcctgacgg aacagctccg
    17161 gcacctggat tgtcgcggcc agcgacgtat cttttaccag actgataaag ctgttcgaca
    17221 gcggcggcaa cgccacccgc gccgcctgtg gcaaaatcgc gcgacgcatg gtctgccacg
    17281 gcgtcatacc aatactggca gcggcttccc actgaccttt atcaatagaa gaaatggcgg
    17341 cgcgcagcgt ttcggcggca taggcggcag tgttcagcga cagaccaatc attgctgaca
    17401 gaatgggatc taattcgata ccaaactgcg gcaggccgta atagatcata aacagttgtg
    17461 cgatgagcgg cgtaccacga aaaatagaga tataaaaacg cgccagccag cgcacgggcc
    17521 agattggcga caggcgcatc agcgcgagaa taaacccgag tagtaagcca aaaaacatgc
    17581 cgccgatact gagttgcagg gtatacccag cccctttcaa caggaacggc aaggaatcaa
    17641 taaccagttg tatactttct tgcatgagcg ttcccggagc taaacgtggg gatgataggc
    17701 gaacagcgca ggcgcgccgc cggtatgaat aaacagaatc ggcccttcat ctttgaagcg
    17761 tttctgactg ataccgtcaa tcagccccgc catcgctttt ccggtataca caggatcaag
    17821 cagaatgcct tccagacgcg ccagcaattt cactgcttcc atgccttcgt cgttcggcac
    17881 gccgtagcca ggtgcaaaat agtcatccca gagtaaaatt tccgctgatg cggtcagctc
    17941 cagttctttc gcaatcgcct gttgtaggtt aaccactttc ggcaattgat cggcaacgga
    18001 acgcgacacg gtcacgccaa tcagttcgct ttcaggcatc aggtgttcca gcccaacagc
    18061 caatccggcg tgagttccgg cactgcccga tgcgaccacc accgacgaaa tattaaccgc
    18121 cccttcacac tgttgcgcga tttccagcgc actctccaca taacccagcg cgcccagagc
    18181 attagaaccg ccaaccggaa tgacatacgg gcgaaagcct tgtgcttcga ctcgcgtcgc
    18241 cagctcttcc agttgggcat tgggatcggt cagtgtgtcg cacatttcaa tctgggtatt
    18301 gaacagattc agcaacaaac gattgccgtt ggttaaatag ttttctgcgg ttgtgccaat
    18361 aggattttcc agcagcgcca cgcagtgcag gccgagtttc gccgcaactg cggcagtctg
    18421 ccgcacatgg ttagactgga tcgcgccggc agtaatcagc gtatcggcac cttcgcgcag
    18481 ggcatctgcc gcgagaaatt ccagcttacg taatttattg ccgcccattg ccatgggtgt
    18541 gacgtcatcc cgtttgatga aaatttcccg tccaagataa tcagaaaagc gcggcagata
    18601 ttcgagcggc gttggcgcgc cgataaactc cagccgtgga aaacgggtta aattatgcag
    18661 tggcataata gcctccgatg tgtgttgttg tgattttctt attatgcacg ctgaaaacgc
    18721 gtaaataaaa aaggcgctag tgaaagcgcc cttttttgtc attatgctga ttatttggtc
    18781 acatcagcac caaaccattt ttcggaaagg gcttgcagag tgccatcttt ttgcatttcc
    18841 gcaattgcat cgttcactgc tttcagcagg tcttcatttc ctttacgcag cgccacgcca
    18901 gactcctgac gggagaatgc ttcaccggtt actgccagcg tatcgttggt tttcttcacc
    18961 agatccagcg ccgccagacg gtcaacgagg atcgcatcga tacggcctac gcgcagatcc
    19021 tgatatttgg tcgggtcatc atcataggta cgcacatcga cgccctgaac attctgccgc
    19081 agccactctt catagttggt gcccagaccg acacccactt ttttgccttt cagatcagca
    19141 gccgttttaa tggtgccttc gttacctttt ttcaccagcg cctgaatacc agaaatggtg
    19201 tacggggtag agaaatcgta ttttttcttg cgctcatcag aaatggtgac ctgattaatc
    19261 accacatcaa tacgtttaga gtccagcgac gctagcatac cgtcccattt ggtcggtttt
    19321 agtgacgcct caacgccaag atgttttgcc agctgttggg caaattccac ttcaaaaccg
    19381 gttaatttgc cgtcatcccc ctgaaaactg aacggcggat aagttccttc cagccctacc
    19441 agtagcgtac cgcgctcttt gactttatta agcagacctt catctgcaaa acttttaacg
    19501 ctcatgcccg caaccagcgc cacggccatc acacccatca atgcctgacg tcccagatgt
    19561 gctaatttca tattcacccc gaatgttgtt atgtctgttt gcagtgtaga gccattgttt
    19621 gtaaacacaa aaacaactcc gctacatctt attcttattt aatatatatc agaagcaggc
    19681 aggctggagg atttctgcac aagtccggta cacgtttgac gctgatagtg ttgatacttc
    19741 cgtaatgcga tgcggtaatt gttggtgctg gtggtgggca gccacggggc gaggatttca
    19801 tcaaggaaac cgtcctgcag ctgatcgagg gaaatatttt gctcatgtag atgattcccc
    19861 aaacggcgca aacgaacgac atattcacgc accgtacctg ggctcatttc agtttgctca
    19921 aaaagaaatt gcttaaagcc gataatatcg aaaaaatcac tctgcgtttt gcaatgtaaa
    19981 tcaccgcaaa atcggcacaa tgccgcccat gactgttgtt ccacttgcca gccattttca
    20041 tcaagcagcg tgtccaggcg ggaaatctcg attttattca ctattttgcc gtcgcgaact
    20101 aaggtaatgc gatcgagtaa tttacggcaa tgcgcgcaat gggcctggct gtgtttaaag
    20161 tctttaagat agcggcttaa tggccgtctt ttcaggtgct gcaccatcat taagaactcc
    20221 tggtagtcaa agttaaagtg cggcatttac tgacgttata acttacccag tttagtgctt
    20281 aaccgtttaa tggcctggct gtgtaactga ctgacccgcg attccccgac ctccagcacc
    20341 gcgccaatct ctttgagatt cagctcttcc tggtaataga gggttaatac cagtttttcg
    20401 cgctccggca acgtttcgat agcttccatc acccgctggc gcagattact gtccagcagt
    20461 tgttgtagcg ggttttctcg ctgatgatca tcagtaacca gttcgatgct atcgccgtgc
    20521 tcttcgcgcc actcatcgta ggagaaaagc tggctgttat tggtgtcgag caacatttgg
    20581 cgataatcgg caatatcgat ccctaaacgt tccgctacct cagtttccgt ggcgttgcgg
    20641 ccaagttcct gctccagttg ccctattgcc tgtgccactt cacgcgcgtt gcgtcgcacg
    20701 ctgcgcggca cccagtcacg gctgcgaagt tcatccagca tagcgccacg gatacgctgc
    20761 actgcgtaag ttgtaaatgc cgttccttgt agggcgtcat agcgttcgac ggcattaagt
    20821 aacccaatgc cgcccgcctg tagcagatcg tcaagttcca cgctcgcggg cagtcgaacc
    20881 tgcaggcgca atgcttcgtg acgcaccagc gggacataac gctgccacag cgagtgttta
    20941 tccattacac cttcagcggt atagagtgaa ttcacgataa acagccctgc gttatatgag
    21001 ttatcggcat gattatccgt ttctgcaggg tttttaatcg gacgattagt gggtgaaatg
    21061 aggggttatt tgggggttac cggtaaattc catgcagaaa aaaccccgcc ggtggcgggg
    21121 aagcacgttg ctgacaaatt gcgctttatg ttgccggatg cggcgtaaac gccttatccg
    21181 gcctacaaaa atgtgcaaat tcaataaatt gcaattcacc ttgtaggcct gataagtgca
    21241 gcgcatcagg caatttggcg ttgccgtcag tctcagttaa tcaggttacg acgattaacc
    21301 ctgcagcaga gacagaacct actgcggtac ctggttggct tttgccagca cggagttacc
    21361 ggcctgctgg atgatctgcg ctttcgacat gttggacact tcggtcgcat agtcggcgtc
    21421 ctgaatacgg gactgcgctt cagacaggtt ggtagtggtg ttgttcaggt tggtgactgc
    21481 agaatccaga cggttctgaa tagcacccag ggatgaacgg aatttgtcga tggagctgat
    21541 agcttcatcc agggcagcca gaggatcttt ggttgcagtg cctgcactgg tggtttcagt
    21601 ggtcagtttg cctgtagagt taacataagc ctgtgcccca acagtagctg caaatttacc
    21661 agttgcacct gcgccatttg aagctacagt tactgtaccg gtatccttat cgacgttgta
    21721 gttgatagtg taagatgcgg ttgtggtgag ctctttgtct gcatcagtat aggtgtcatt
    21781 cgaattacca gcaccattag tattcgcacc accgaaggac gccgttgcag actgaatacc
    21841 tgtattatac ttaattgtcg caccagtaac tgctgtgtta gccgttgcag cactctgcac
    21901 tgtgctcagt acagtatcag cgcttacagt tttggtgtag gttacgccag aagttgccgc
    21961 tgcaccaaag ttataagttg tgccagacaa ctggatagaa ccagttgtcg ctaaggtact
    22021 accgctagca aacagatcgg acaatttcgc tgaagatgca atgccggcgt tgtttgtaga
    22081 taggttacca gtggcgtcca ggtacgcggc tttaccaccg atggtcacat cgccattagc
    22141 atctacatca aacttcacat caccagtact acgagtataa acacctgatg cagtagtgcc
    22201 agccgctggt ttcagagtat tcgcaaagtt aacaacatcg ccatctgcaa ctgttgcttg
    22261 agtggtgaag ttccctttag ccgcatcata agtatagatc gcagcactcg agccagtagt
    22321 agtaactgta tctccggttt tcaggcgaga cagtgcatcg ctagcgctga gtgctgtatt
    22381 gtttgtggtc acagcataag gacctgttcc cgttgcacca gcagcggtca gatcgctgac
    22441 tgtagcagct ttgttcgcaa tagtgccttt gccattcacg ttaaaaccat tcagccccag
    22501 cgtatctgag tcaattttct tcagatcaat cgtgatagtc tggccgtcat tcgcaccaac
    22561 ctgaattttc attgaaccgt ctttcgccag tacgttcacg ccgttgaact gggtctggcc
    22621 agatacgcgg tcaatttcgt ccagacggga tttgatttcg tcctgaatgg agtccagatc
    22681 cgaatcggag ttagtcccgg tagaagcctg aaccgtcagt tcaagaatac gctgtaagtt
    22741 gttgttgatt tcggacagcg cgccttcagt ggtctgcgca acggagatac cgtcgttggc
    22801 gttacgggcc gcctgagtca ggcctttaat attagaagta aaacggttag caatcgcctg
    22861 acctgcggcg tcatccttcg cgctgttaat acgcaagcca gaagacagac gctcgataga
    22921 actcgacagc gcagactggt tcttgttgat attattttga gtgatcagcg agaggctgtt
    22981 ggtattaatg acttgtgcca tgattcgtta tcctatattg caagtcgttg attacgtatt
    23041 gggtttccac ccgtcggctc aatcgccgtc aaccctgtta tcgtctgtcg taaaacaacc
    23101 tttagaattt ttttcacaaa cagccatttt ttgttagtcg ccgaaatact cttttctctg
    23161 ccccttattc ccgctattaa aaaaaacaat taaacgtaaa ctttgcgcaa ttcaggccaa
    23221 taaccccggt attcgtttta cgtgtcgaaa gataaaagga aatcgcatgg caagtatttc
    23281 atcgctggga gtcgggtcag gtctggattt aagttcaatc cttgatagcc tcaccgccgc
    23341 gcaaaaagcg acgctaaccc ctatttcaaa tcagcaatcg tcgtttaccg ctaaacttag
    23401 cgcctacggt acgctgaaaa gcgcgctgac gactttccag accgccaata ctgcattgtc
    23461 taaagccgat cttttttccg ccaccagcac taccagcagc accaccgcgt tcagtgccac
    23521 cactgcgggt aacgccatcg ccgggaaata caccatcagc gtcacccatc tggcgcaggc
    23581 gcaaaccctg accacgcgca ccaccagaga tgatacgaaa acggcgatcg ccaccagcga
    23641 cagcaaactc accattcaac aaggcggcga caaagatccg attaccattg atatcagcgc
    23701 ggctaactca tcgttaagcg ggatccgtga tgctatcaac aacgcaaaag cgggcgtaag
    23761 tgcgagcatc attaacgtgg gtaacggtga atatcgtctg tcagtcacat caaatgacac
    23821 cggccttgat aatgcgatga cactctcggt cagcggtgat gatgcgctac aaagttttat
    23881 gggctatgac gccagtgcca gcagcaacgg tatggaggtc tcggtcgccg ccaagaatgc
    23941 gcagctgacg gtcaacaacg tcgccattga gaacagcagc aacaccatca gcgacgcgct
    24001 ggaaaacatc accctgaacc tgaacgatgt caccacgggc aaccagacgc taaccatcac
    24061 tcaggacacc tccaaagcgc aaacggcgat taaagactgg gtgaatgcct ataactcgtt
    24121 aatagatacc ttcagtagcc tgaccaaata caccgccgta gatgcgggag ctgatagcca
    24181 gagttctagc aatggcgcac tgctcggcga ctccacgctg cggacgattc agacgcagtt
    24241 gaagtcgatg ctgagtaata ccgtcagttc ttccagctat aaaacgctgg cgcagattgg
    24301 tatcacgacc gatcccagcg atggcaaact ggaactggat gccgacaaac tcaccgctgc
    24361 actgaaaaaa gatgccagcg gcgtaggtgc attgattgtt ggcgatggta aaaaaaccgg
    24421 catcacgacc accatcggca gcaacctgac cagttggctt tcgacaacgg gcattattaa
    24481 agccgctacc gatggcgtta gtaagaccct gaataagtta actaaagact acaacgccgc
    24541 cagcgatcgc attgatgcgc aggtcgcgcg ctacaaaaaa caatttaccc aactggacgt
    24601 tttaatgacc tcgttaaaca gcaccagcag ctacttaacg cagcagttcg aaaacaacag
    24661 taattccaag taatcattat tcatcgggag acaggtcatg tacgcggcaa aaggcaccca
    24721 ggcctatgca caaattggcg tcgaaagcgc cgtaatgagc gccagccagc agcagctggt
    24781 caccatgcta tttgatggag tgctgagcgc actggttaga gcgcgcctgt ttatgcagga
    24841 caacaatcag caaggcaaag gcgtctcttt gtcaaaagcg atcaacatca ttgagaacgg
    24901 actgcgggtg agtcttgatg aagagagcaa agacgaacta acccaaaact tgattgctct
    24961 ttatagctat atggtcaggc gcttgctgca agccaattta cgcaacgatg tctccgcagt
    25021 cgaagaagtg gaagcattaa tgcgcaatat tgccgatgcc tggaaagagt cgttactctc
    25081 cccttctttg attcaggacc cagtctgatg aaccatgcac cgcatttgta tttcgcctgg
    25141 caacaactcg tcgaaaaaag ccagctcatg ttacgcctgg caacggaaga acaatgggac
    25201 gaactcatcg ccagcgaaat ggcgtatgtg aatgcggtgc aggagtatag cacacctgac
    25261 tgaagaggtt gccccgtcca ccacgatgca ggagcagctc cgcccgatgc tgcgcctgat
    25321 tctcgacaac gaaagcaagg taaagcagtt attacagatt cgtatggatg aactggcgaa
    25381 actggtcggt cagtcatcgg tgcaaaaatc ggtgttaagt gcctatggcg atcagggcgg
    25441 ctttgtgctg gctccgcaag ataacttctc gtgaatctta acgagctaat ggttcgcgaa
    25501 taatccgatt acggctacgc ttctaatgtt ccccttgaat ggagtcgaag aatgcgtaat
    25561 cccacgctgt tacaatgttt tcactggtat tacccggaag gcggtaagct ctggcctgaa
    25621 ctggccgagc gcgccgacgg ttttaatgat attggtatca atatggtctg gttgccgccc
    25681 gcctataaag gcgcatcggg cgggtattcg gtcggctacg actcctataa tttatttgat
    25741 ttaggcgagt ttgatcagaa aggcagcatc cctactaaat atggcgataa agtacaattg
    25801 ctggccgcca ttgacgccct gaaacgtaat gacattgcgg tgctgttgga cgtggtagtc
    25861 aaccacaaaa tgggcgcgga tgaaaaagaa gctattcgcg tgcagcgtgt aaatgctgac
    25921 gaccgtacgc aaattgatga agaaatcatt gagtgcgaag gctggacgcg ttacaccttc
    25981 cccgcccgtg ccgggcaata ctcacagttt atctgggatt tcaaatgttt tagcggtatc
    26041 gaccatatcg aaaaccctga tgaagatggc atatttaaaa ttgttaacga ctacaccggc
    26101 gaaggctgga acgatcaggt tgatgatgaa ttaggtaatt tcgattatct gatgggcgag
    26161 aatatcgatt ttcgcaatca tgccgtgacg gaagagatta aatactgggc gcgctgggtg
    26221 atggaacaaa cgcaatgcga cggttttcgt cttgatgcgg tcaaacatat tccggcgtgg
    26281 ttttataaag agtggatcga acacgtacag gaagttgcgc caaagccgct gtttattgtg
    26341 gcggagtact ggtcgcatga agtcgataaa ctgcaaacgt atattgatca ggtggaaggc
    26401 aaaaccatgc tgttcgatgc gccgctgcag atgaaattcc atgaagcatc gcgcatgggg
    26461 cgcgactacg acatgacgca gattttcacg ggtacattag tggaagccga tcctttccac
    26521 gccgtgacgc tcgttgccaa tcacgacacc caaccgttgc aagccctcga agcgccggtc
    26581 gaaccgtggt ttaaaccgct ggcgtatgcc ttaattttgt tgcgggaaaa tggcgttcct
    26641 tcggtattct atccggacct ctacggtgcg cattacgaag atgtcggtgg tgacgggcaa
    26701 acctatccga tagatatgcc aataatcgaa cagcttgatg agttaattct cgcccgtcag
    26761 cgtttcgccc acggtgtaca gacgttattt ttcgaccatc cgaactgcat tgcctttagc
    26821 cgcagtggca ccgacgaata tcccggctgc gtggtggtca tgtcgaacgg ggatgatggc
    26881 gaaaaaacca ttcatctggg agagaattac ggcaataaaa cctggcgtga ttttctgggg
    26941 aaccggcaag agagcgtaat aaccgacgaa cacggcgaag caaccttctt ttgcaacggc
    27001 ggcagcgtca gcgtgtgggt tatcgaagag gtgatttaaa ttcatccccg gcggcaagcc
    27061 ggggagattt cattacggca gtggcgtcgg taacggcgct ttatccagcg cggcggcaca
    27121 ctctgccgtc gggcgatcca cacgctccat cgtcatacca tcgtactcaa tggtgttacc
    27181 gtcacgctca acctcgtaca gctcacgttt tacggtaacg ttagtgagat catcagacat
    27241 caatgccaac ttacccggca cagcaatcac ccgctgccac tgacggcaat ccagagtatc
    27301 accctcttta gtgacaatca ggctgcctat cgcttccgga ctgaccagcg cacgttgcgg
    27361 ccctttggtc tgccagtagc ccgccagcca gtccggcgcg ggggttttca cgacattgtt
    27421 atagttttcg acttcggcac agcccgccag caacatgagc gcgcctgcaa ttgcgagttt
    27481 tttcatcatt cgtcctgcat gagaagaaaa gaggattgtg gcattaaagc cctgatgtcg
    27541 ccagcctttc caggttcaga gagaaatttg atctaactat tatttttgtg aattattacc
    27601 ccgaaaataa atttgcatga tgtatgattc gcgactcttg tttcaaacct tctgagttca
    27661 gaggctacat tcatgtcatg gcagcaattc aaacacgcct ggttgattaa attctgggca
    27721 cccatccctg cggtcatcgc ggcgggtatt ctctccactt actattttgg cattactggc
    27781 accttttggg ctgtcacggg tgaatttacc cgttggggcg gccagctcct gcagctgttt
    27841 ggcgtccatg ctgaagagtg gggttatttt aaaattatcc atctggaagg atcgccatta
    27901 acccgcatcg acgggatgat gatcctcggc atgtttggcg gctgctttgc cgcagcgctg
    27961 tgggccaaca atgtcaaact gcgcatgcca cgcagccgta tccgcattat gcaggccatt
    28021 attggcggta ttatcgctgg ttttggcgcg cgtctggcaa tgggctgtaa cctggcggcg
    28081 ttctttaccg gtattcctca gttctcgctg catgcctggt tctttgccat cgccactgcc
    28141 attggttcat ggtttggcgc acgctttacc ctgctgccca tcttccgtat tcccgtgaaa
    28201 atgcagaaag tttctgccgc ctcaccgctg acgcaaaaac cggatcaggc gcggcgtcgt
    28261 tttcgtctcg ggatgctggt ctttttcggt atgctgggct gggcgctgct cacggcgatg
    28321 aaccaaccaa aactggggct ggcaatgctg tttggcgtcg gctttggttt actgattgaa
    28381 cgtgcgcaaa tctgctttac ttcggcgttc cgcgatatgt ggatcaccgg acgtacccat
    28441 atggcgaaag caatcattat cggtatggcg gtgagtgcca tcgggatctt cagttacgta
    28501 cagttaggcg ttgaacccaa aatcatgtgg gcgggaccaa acgcggtaat tggtggttta
    28561 ctgtttggtt ttggcatcgt gctggcaggc ggctgcgaaa ccggctggat gtaccgcgcg
    28621 gtagaaggcc aggtgcacta ctggtgggtc ggtctgggca atgtgatcgg ctcgacgatt
    28681 ctggcgtatt actgggatga tttcgctccg gcgctggcca ccgactggga caaaatcaac
    28741 ctgctgaaaa cctttggccc gatgggtggc ctgctggtga catatttgct gttgtttact
    28801 gcgctgatgt tgattatcgg ctgggaaaaa cgcttcttcc gccgtgcggc accgcagact
    28861 gctaaggaga tcgcatgaaa aatatcgttc ctgattaccg ccttgatatg gtgggtgaac
    28921 cctgccctta tccggcagtc gcaacccttg aggcgatgcc gcagttaaaa aaaggggaaa
    28981 tcctggaagt ggtgagcgac tgtccacagt cgatcaataa tattccactg gatgcgcgta
    29041 atcacggcta tacggtgcgg gatattcagc aagacgggcc gaccattcgt tatttaattc
    29101 agaagtaatc cctcattccc gctgtggccc acagcgggaa catcgttgcc tctgacacat
    29161 agattttacc cgccccctct cctattcttt tcagtatgat ctcaggaggt ttctatgtgt
    29221 ggacgctttg cccaatccca aacgcgtgaa gattaccttg cgcttctcgc ggaagatatt
    29281 gaacgcgata ttccctacga tcccgaaccc attggcagat acaacgtcgc gccgggaacc
    29341 aaagttcttc tgctcagtga acgtgatgaa caccttcatc tggatccggt tttctgggga
    29401 tatgctcccg gatggtggga taaaccgccg ctgattaacg cccgcgtaga aactgccgca
    29461 accagtcgca tgtttaaacc gctctggcaa catggtcggg caatctgttt tgccgatggc
    29521 tggtttgagt ggaaaaaaga aggcgacaaa aaacagcctt tttttatcta tcgtgctgat
    29581 ggacaaccta tttttatggc cgcgataggt agcacaccat ttgagcatgg tgacgaagcc
    29641 gaaggatttt taattgtcac tgctgcagca gaccagggtc tggtagacat tcatgaccgc
    29701 cgcccgctgg ttctgtcgcc agaagctgca caggaatgga tgcggcaaga gattggcggt
    29761 aaagaagcat cagaaattgc taccaacggc tgtgtgccag caaaccagtt cacctggcac
    29821 ccggtatcgc gcgcggtagg taatgtcaaa aaccagggcg cggagttaat tcaacctgtt
    29881 tgttgacatc ctccacgccc tgaaggacga ggctccctgc tacgttcagg ctgtcgcctg
    29941 aatcatttcg gtgggttcct gcttcaacgg gcggcctgac tgcaccatc
//
LOCUS       DAJVUJ010000031        29254 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.031, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000031 DAJVUJ010000000
VERSION     DAJVUJ010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29254
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.031"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..155
                     /locus_tag="OQQ48_001312"
     CDS             <1..155
                     /locus_tag="OQQ48_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2135 domain-containing protein"
                     /protein_id="HCS1952146.1"
                     /translation="NYYGGRSETELTTAQLTLITDEGSVNEKQETFIVPMRNAGELTL
                     VKSFDW"
     gene            430..3279
                     /gene="rcsC"
                     /locus_tag="OQQ48_001313"
     CDS             430..3279
                     /gene="rcsC"
                     /locus_tag="OQQ48_001313"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HCS1952147.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGEPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(3479..4129)
                     /gene="rcsB"
                     /locus_tag="OQQ48_001314"
     CDS             complement(3479..4129)
                     /gene="rcsB"
                     /locus_tag="OQQ48_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HCS1952148.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(4146..6818)
                     /gene="rcsD"
                     /locus_tag="OQQ48_001315"
     CDS             complement(4146..6818)
                     /gene="rcsD"
                     /locus_tag="OQQ48_001315"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HCS1952149.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVNTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTISDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGDNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQPQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            7557..8687
                     /gene="ompC"
                     /locus_tag="OQQ48_001316"
     CDS             7557..8687
                     /gene="ompC"
                     /locus_tag="OQQ48_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCS1952150.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKSEDGDQTYVRLGFKGETQVTDQLTGYGQWEYQIQGNTSEDNKENSWTRVAFAGL
                     KFQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFG
                     LVDGLNFAVQYQGKNGSVSGEGMTNNGRGALRQNGDGVGGSITYDYEGFGIGAAVSSS
                     KRTDDQNGSYISNGVVRNYIGTGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSL
                     GWANKAQNFEAVAQYQFDFGLRPSVAYLQSKGKNLGVINSRNYDDEDILKYVDVGATY
                     YFNKNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            8799..9854
                     /gene="apbE"
                     /locus_tag="OQQ48_001317"
     CDS             8799..9854
                     /gene="apbE"
                     /locus_tag="OQQ48_001317"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HCS1952151.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MAEIVTTSLRIGAKTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDEMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GINGEGLPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            9928..10980
                     /gene="ada"
                     /locus_tag="OQQ48_001318"
     CDS             9928..10980
                     /gene="ada"
                     /locus_tag="OQQ48_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HCS1952152.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANAQQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCALGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVLANAIGKPKAVRAVASACAANKLAIVIPCHRVVGGDGTLSGYRWGV
                     SRKAQLLRREAENEER"
     gene            10980..11630
                     /gene="alkB"
                     /locus_tag="OQQ48_001319"
     CDS             10980..11630
                     /gene="alkB"
                     /locus_tag="OQQ48_001319"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HCS1952153.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTIHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFYPLTTDCRYNLTFRQAGKKE"
     gene            11706..13349
                     /gene="yojI"
                     /locus_tag="OQQ48_001320"
     CDS             11706..13349
                     /gene="yojI"
                     /locus_tag="OQQ48_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HCS1952154.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIEMA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLMAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFSYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGKPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            13567..15213
                     /gene="mqo"
                     /locus_tag="OQQ48_001321"
     CDS             13567..15213
                     /gene="mqo"
                     /locus_tag="OQQ48_001321"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HCS1952155.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMLERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDADKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
     gene            complement(15363..15851)
                     /gene="eco"
                     /locus_tag="OQQ48_001322"
     CDS             complement(15363..15851)
                     /gene="eco"
                     /locus_tag="OQQ48_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HCS1952156.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAEKGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAE
                     VR"
     gene            complement(15986..16150)
                     /locus_tag="OQQ48_001323"
     CDS             complement(15986..16150)
                     /locus_tag="OQQ48_001323"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1952157.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCSLIRI"
     gene            16259..16753
                     /gene="napF"
                     /locus_tag="OQQ48_001324"
     CDS             16259..16753
                     /gene="napF"
                     /locus_tag="OQQ48_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HCS1952158.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            16743..17006
                     /gene="napD"
                     /locus_tag="OQQ48_001325"
     CDS             16743..17006
                     /gene="napD"
                     /locus_tag="OQQ48_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HCS1952159.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            17003..19489
                     /gene="napA"
                     /locus_tag="OQQ48_001326"
     CDS             17003..19489
                     /gene="napA"
                     /locus_tag="OQQ48_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HCS1952160.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTLITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEDLLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTQDATDPLSKETDFKKCAVKLEKV"
     gene            19496..20191
                     /gene="napG"
                     /locus_tag="OQQ48_001327"
     CDS             19496..20191
                     /gene="napG"
                     /locus_tag="OQQ48_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HCS1952161.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHRDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            20178..21041
                     /gene="napH"
                     /locus_tag="OQQ48_001328"
     CDS             20178..21041
                     /gene="napH"
                     /locus_tag="OQQ48_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HCS1952162.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWI
                     NPVSLMGRSLVVGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AATDRQKCNRCMDCFHVCPEPRVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            21038..21487
                     /gene="napB"
                     /locus_tag="OQQ48_001329"
     CDS             21038..21487
                     /gene="napB"
                     /locus_tag="OQQ48_001329"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HCS1952163.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQERMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            21497..22099
                     /gene="napC"
                     /locus_tag="OQQ48_001330"
     CDS             21497..22099
                     /gene="napC"
                     /locus_tag="OQQ48_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HCS1952164.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            22118..22735
                     /gene="ccmA"
                     /locus_tag="OQQ48_001331"
     CDS             22118..22735
                     /gene="ccmA"
                     /locus_tag="OQQ48_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HCS1952165.1"
                     /translation="MLEARELLCEQDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHRDG
                     DTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            22732..23394
                     /gene="ccmB"
                     /locus_tag="OQQ48_001332"
     CDS             22732..23394
                     /gene="ccmB"
                     /locus_tag="OQQ48_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HCS1952166.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            23436..24173
                     /gene="ccmC"
                     /locus_tag="OQQ48_001333"
     CDS             23436..24173
                     /gene="ccmC"
                     /locus_tag="OQQ48_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HCS1952167.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            24170..24379
                     /gene="ccmD"
                     /locus_tag="OQQ48_001334"
     CDS             24170..24379
                     /gene="ccmD"
                     /locus_tag="OQQ48_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HCS1952168.1"
                     /translation="MTLAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILCGVEQQRAREARLRAAQQQEAA"
     gene            24376..24855
                     /gene="ccmE"
                     /locus_tag="OQQ48_001335"
     CDS             24376..24855
                     /gene="ccmE"
                     /locus_tag="OQQ48_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HCS1952169.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            24852..26795
                     /gene="ccmF"
                     /locus_tag="OQQ48_001336"
     CDS             24852..26795
                     /gene="ccmF"
                     /locus_tag="OQQ48_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HCS1952170.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            26792..27349
                     /gene="dsbE"
                     /locus_tag="OQQ48_001337"
     CDS             26792..27349
                     /gene="dsbE"
                     /locus_tag="OQQ48_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HCS1952171.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            27346..28398
                     /gene="ccmH"
                     /locus_tag="OQQ48_001338"
     CDS             27346..28398
                     /gene="ccmH"
                     /locus_tag="OQQ48_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HCS1952172.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPDAFPEQSVPEGKRAGYIVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLSANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(28433..29080)
                     /gene="narP"
                     /locus_tag="OQQ48_001339"
     CDS             complement(28433..29080)
                     /gene="narP"
                     /locus_tag="OQQ48_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HCS1952173.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPSFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
BASE COUNT         6680 a         7221 c         8103 g         7250 t
ORIGIN      
        1 tcaactacta tggtgggcgc agcgaaacgg aattaaccac cgcccaactg acgctgatca
       61 ccgatgaagg atcggtcaat gagaaacagg aaacatttat tgtaccgatg cgtaatgctg
      121 gcgagctgac gctggtgaaa agttttgact ggtgatccat cccttcacac aacctggctc
      181 tctctttttt cagataagac tgctgggcga tctgccgacg ccaggatata accattaaca
      241 agcgtcggca gattggtcat ttcttatttt tcgtactgag aactgtccat ctactccggc
      301 tttcctggtt aaggcaatgg tttctaagcg atacgcatta ttgcaataat gacagaacat
      361 tgcggtaaca cgcttttacc gctaccttaa ccacactcca tcggtcacct gaggcggagc
      421 ttcgcccctt tgaaatacct tgcttctttt cgtacaaccc tgaaagcctc gcgctacatg
      481 ttcagagcat tggcgttagt gctctggctg ttgattgctt tttcatccgt tttttacatc
      541 gttaatgcgt tacatcagcg agaatcggaa attcgtcagg aatttaatct gagttccgat
      601 caggctcagc gctttattca acgcacctct gatgtgatga aagagctgaa gtacatcgcc
      661 gaaaatcgct tatcggcaga aaacggtgtg ctttccccgc gtggacgaga aacgcaggcg
      721 gatgtgcctg cgtttgaacc gctgtttgcc gactccgatt gttccgcaat gagtaacacc
      781 tggcgaggtt ctctggagtc attggcgtgg tttatgcgct actggcgcga taatttttct
      841 gcggcttacg atctaaaccg ggtattttta atcggcagcg ataacctctg catggccaat
      901 ttcggtctgc gtgatatgcc agtggaacgc gataccgcgt tgaaagcttt gcatgaacgc
      961 atcaataaat atcgaaatgc accacaagat gatagcggca gtaacctcta ctggatcagc
     1021 gaaggtccgc gccctggcgt cgggtatttt tacgcgttga cgccagttta tctggcgaac
     1081 cggttgcagg cgcttttggg ggtcgagcag accatccgga tggagaactt tttcttaccg
     1141 ggtacgttgc cgatgggggt taccattctt gatgaaaatg gtcataccct gatttcgctt
     1201 accggaccag aaagtaaaat taagggcgag cctcgctgga tgcaggaacg ctcctggttt
     1261 ggctatacgg aagggttccg ggagctggtg ctgaagaaaa atctgccacc ctcatcgcta
     1321 agcatcgtgt attcggtgcc ggttgataag gtgctggaac gcattcgcat gttgatcctt
     1381 aacgcaattt tgctgaatgt gcttgccgga gctgcattgt ttactctcgc acggatgtac
     1441 gagcgacgta ttttcattcc ggcggaaagc gacgccctgc gactggaaga acatgagcag
     1501 ttcaatcgca agattgtcgc ctccgcgcca gtgggtatct gcattttgcg taccgctgat
     1561 ggcgtcaata ttttaagtaa cgaactggcg catacctatc tcaatatgct tacgcatgag
     1621 gaccgccaac gactgacgca aattatctgt gggcagcagg tcaattttgt tgatgtcctg
     1681 accagcaaca ataccaatct gcaaatcagc ttcgtccatt cgcgctatcg taatgaaaac
     1741 gtggccattt gtgtgctggt ggatgtttct tcgcgcgtga agatggaaga gtcgttgcag
     1801 gagatggcac aagcagcgga acaggcgagc cagtcaaaat cgatgttcct tgccaccgtc
     1861 agtcatgagc tgcgaacgcc gctgtatggc attatcggta acctggatct gttgcaaacc
     1921 aaagagttac cgaaaggcgt cgatcggctg gtgacggcaa tgaacaactc ttccagcctg
     1981 ttgttgaaaa ttatcagcga tattctcgat ttctcgaaga ttgaatcgga acagttgaag
     2041 atcgaaccgc gtgagttttc accgcgtgaa gtgatgaacc acatcaccgc caactattta
     2101 ccgctggtgg tacgcaagca gttaggcttg tactgcttta ttgaaccgga tgtgccagtg
     2161 gccttaaatg gcgacccgat gcgtttacag caggtcatct ccaacctgtt gagtaacgcc
     2221 ataaaattca ccgataccgg ctgtatagtt ttgcatgtcc gcgctgatgg cgattatctc
     2281 tctatccgtg ttcgcgatac cggcgtgggg attccggcga aagaagtggt gcgcttgttt
     2341 gatcccttct tccaggtcgg aacgggcgta cagcgaaact tccaggggac cggtctgggt
     2401 ctggcgattt gtgaaaaact gatcagcatg atggacggcg atatctcggt agattcagaa
     2461 ccgggaatgg gcagccagtt taccgtgcgt attccgttgt acggcgctca gtacccgcag
     2521 aaaaaaggcg tggaagggtt gagtggtaaa cgctgctggc tggcggtccg caatgcgtcg
     2581 ctctgtcagt tcctggaaac tagtttgcag cgcagcggca tcgtcgttac aacatacgaa
     2641 gggcaggaac cgactcccga agatgtgtta attactgacg aggtagtgag taaaaaatgg
     2701 cagggtagag cggtagtgac cttctgtcgt cgccatattg gtattccgct ggagaaagcg
     2761 ccaggggagt gggtacacag tgtggctgcc ccgcatgagc taccggcatt gttggcgcgt
     2821 atttatttga tcgagatgga gagcgacgat cctgctaacg ctctgccgtc gacggacaaa
     2881 gcggtcagcg ataatgacga tatgatgatt ctggtcgtgg atgatcatcc gattaaccga
     2941 cgtctgctgg cagatcagtt gggatcgttg ggctatcaat gtaaaaccgc gaatgatggt
     3001 gtcgatgcgc ttaatgtact tagcaagaat catattgata tcgtgcttag cgacgtcaac
     3061 atgccaaata tggatggtta ccgcttgacg caacgcattc gtcagttggg actgacgttg
     3121 ccggtaatcg gagtaactgc taatgcgttg gctgaagaga agcagcggtg tctggagtcc
     3181 ggtatggaca gctgcctgtc gaagccggta acgctggatg tgataaaaca gacgctgacg
     3241 gtatatgccg agagggtcag gaaatcgcgg gaatcgtagg acggataagg tgtttatgtc
     3301 gcatctggca ttcagtgcaa atgccagatg cgacgctgac gcgtcttatc tggcctactt
     3361 tgatgtaatc tgttgaaata atgggaatcg taggacgcat aaggcgttta cgccgcatcc
     3421 ggcaatcggt gcaaatgcca gataagacac taacgcgtct tatctggcct acaggtgatt
     3481 agtctttatc tgccggactt aacgtcactg aagagagata attcagcagg gcgatatcgt
     3541 tctcgacacc cagcttcatc atcgcagatt tcttctggct actgatggtt ttaatactgc
     3601 ggttcagctt tttagcgatc tcggtcacca agaagccttc cgcaaacagg cgcagaactt
     3661 cactctcttt tggcgagaga cgcttgtcac cgtaaccacc agcactgatt ttttccaaca
     3721 ggcgagaaac gctttccggg gtaaatttct tccctttctg cagcgcggcg agagctttcg
     3781 gcagatcggt cggtgcacct tgtttcagca cgatcccttc gatatccaga tccaataccg
     3841 cactaagaat cgccgggttg ttgttcatag tcagaacaat gatcgacagg cttgggaaat
     3901 ggcgcttgat gtacttgatt aaggtaatgc catcgccgta cttatcgcca ggcatggaga
     3961 gatcggtaat caacacatgc gcatccagtt tcggcaggtt gttgatcagt gctgtagagt
     4021 cttcaaattc gccgacaaca ttcacccact caatttgctc aagtgatttg cgaataccga
     4081 acaagactat cggatggtca tcggcaataa ttacgttcat attgttcatg taataggcta
     4141 ccttgctaca gcaagctctt gacataactg tcaatgtcgc tgatgtattt ttctattcct
     4201 ggaacatcct tctcacgaat cagatgttcc agcgtttcac ataactgctt gccgggtacc
     4261 agatttagca tggcaaatac gcctttaaga cgatgagccg tttgggctaa cgcagcaaag
     4321 tcactggttg ctgcttcagt atacagcctc ttaacatcat ccggtactgt gtctacaaag
     4381 agcgcataat agccgctggc atggagttgc gcattttcat ctccgcccag aggcgattct
     4441 gtcacctctt cctgcgccag ttgcacttca attaattgta agaccgcttc ctgcatagcg
     4501 ttgctcatat tgaagttgac gcacaattga ccaggcccaa tttcccgtac gccagactca
     4561 tcatcgctta aaagcaagcc agaggcagta agattagacg gattatccgt taaaaagata
     4621 tcataatctt gactaattaa tctttcatcg ggtgtgatac aggttgcacc ccaattttct
     4681 aactggcgag tgacaatatt ccgaatttct gccgaagtaa catccaccat tacgcagaca
     4741 tcatccagta aacgctcttc ttcctcttca acttccgggt cagcggcgag cattttgata
     4801 tgcacagagt agcgtgtacc aagcccatcc cgcgttttga tgtttaaatg accgcccagt
     4861 ttacgcgcca gttgatcgct cagccagaat gccagcgggt ccgccttgcc atagcgatcg
     4921 ttttggggct ggttgataaa cgggaagtgc aaattatcca tttcatgaat acttacgcct
     4981 tctcccgtgt ccagaatgcg gaatgtcagg cggtcttcag aggactcatc ctgatcaacc
     5041 tcaagggtga tttttcccaa ttgcgttgag gtcacggcat attgcatcag tagcagcaaa
     5101 atacgtcgta aggcatcgcg atcgccgcgg cgcatatcgt gtgctttcag atgattgtta
     5161 atcagcagtt gcagaccttt acgcttgatg gcaggcaaca ctgaaggcac aacttcatca
     5221 attaaatcct gcacggagaa cagcaccgtc tcacttttcc agctatcgtc cgcaagcatg
     5281 ttcgctaact gtatttcatc gaccagccgc accagcacat ctgcctgatt cgccagttgt
     5341 ttgctttccg gggcgttgag tttagccgcg ctctccgcca gggactgtgc gggttctttc
     5401 agcgcatcgc caatgttttt cataaaggtc atccgcccct gctggttttt ctcatacaga
     5461 cgctgcgcct gcttgagttt cttgtttacc agcacttcgc gatcctgatc gcgaataatg
     5521 aaaatttgtg tgcgcggcgc gacctggctg cggaacatgc ggatctcata cagctcgtta
     5581 ttgatcgtcg cctgaataat cccctgatgc tgttccgcca tggtggtgat gttttgcaga
     5641 ttcaaatgcg gcagcaaatg atcggcaatt ttgttactta tgacagtgcg gttcgattcc
     5701 tgatcgtgaa ccagcaggcc gagcggcagc agtgagacta tctcttcatt aattgcccgt
     5761 aaaatgcgca attcgttatt gaccgccgtg ctgggtagac tttctgtact gcggctggag
     5821 aaatggcgga atgtggtata gccaaataac gccagcgcca gcaaaccgat gttcagcagc
     5881 agtggcagca gaatgttttg caacgtatcc agcaataagg tgccataagg aacctgccag
     5941 accaggcgca tatcggtaga gttgagtgcc gaggagattt caatcttcgt actgttaaag
     6001 tggatactga cgctatccgt cccttctttc tcattatcat tgtctcccgt tgccgtcgcg
     6061 tctggctcaa ggcggaaact gtccagcggc atacccggtg ggatcaaatc attaatcggt
     6121 agatcaaaag ctacgaccgt tgccagatgt cccggctggt tgaacgtggt acgcaaggta
     6181 aagtaatgac cgttctgcca ggccaggcgg cgcatgttag aaaaactttc gcgttcatcg
     6241 agggcgttgg cctgttgcaa catctctgca cgacgtgaat caacaatgtc gctgatggtc
     6301 gattctttaa atccggaggt gagatctttg aggggcaggg ttgagatcag caccagactg
     6361 ttatcctgac cattcaggta atacatcgac cacggtacat tttctgcgcc ccacaatgtg
     6421 tccagatagg tggacatccg ctgagtcatc tcaagcgttg agttgtcgtg agagccaaag
     6481 attaacgctt ccgttttgcg gcgcggtttc tccagatagt agacatcctg tttcaggcgc
     6541 gtctcttgta aaccttcgcc ggaggagggg gaggttgtcg cggcaatgtt gtcgtagatc
     6601 tgccaggtca cgtaacgcca ggtattgacg cgcttttgga tagcgtgggt aatgtcgaca
     6661 atctggtaac ttttatcttt cagccatgcg ttaacggcgc tttgtaccat tacacccatc
     6721 gtcaccagta acacaatgat caacagtaaa aagaagcggg taatgctccc cggtaggagt
     6781 gaaaagcggg tcgtggccgt tgtctctttc tgacgcattc gtgtttatga cctgttaaaa
     6841 cttcgcgaag tgatagggca gtataaaggg tacagtgtga aattccagac tcttacctga
     6901 ttgatgattc tctattctcc cgtgatattt atcatcctgc attgggggaa atagatgtac
     6961 aagcgccatt ttgctgtaca aataacctac aaaaagccca acgaaattaa ttgcaaataa
     7021 acgatgattg ctggaaatta tgctgatgtt aattatttgt gaaatagtta acaagcgtta
     7081 tagtttttct gtggtagcac agaataatga aaagtgtgta aagaagggta aaaaaaaccg
     7141 gatgcgaggc atccggttga aataggggta aacagacatt cagaaatgaa tgacggtaat
     7201 aaataaagtt aatgatgata gcgggagtta ttctagttgc gagtgaaggt tttgttttga
     7261 cattcagtgc tgtcaaatac ttaagaataa gttattgatt ttaaccttga attattattg
     7321 cttgatgtta ggtgcttatt tcgccattcc acaataatct taaaaagttc ccttgtattt
     7381 acattttgaa acatctatag cgataaatga aacatcttaa aagttttagt atcatattcg
     7441 tgttggatta ttctgcattt ttggggagaa tggacttgcc gactgattaa tgagggttaa
     7501 tcagtatgca gtggcataaa aaagcaaata aaggcatata acagagggtt aataacatga
     7561 aagttaaagt actgtccctc ctggtcccag ctctgctggt agcaggcgca gcaaacgctg
     7621 ctgaagttta caacaaagac ggcaacaaat tagatctgta cggtaaagta gacggcttgc
     7681 actatttctc tgacaacaag tcagaagacg gcgaccagac ctatgtacgt cttggtttca
     7741 aaggcgaaac tcaggttact gaccagctga ccggttacgg ccagtgggaa tatcagatcc
     7801 agggcaatac ctctgaagac aacaaagaaa actcctggac ccgtgtggca ttcgcaggtc
     7861 tgaaattcca ggatgtaggt tctttcgact acggtcgtaa ctacggcgtt gtttacgacg
     7921 taacttcctg gaccgacgta ctgccagaat tcggtggcga cacctacggt tctgacaact
     7981 tcatgcagca gcgtggtaac ggcttcgcga cctaccgtaa caccgacttc ttcggtctgg
     8041 ttgacggtct gaactttgct gttcagtacc agggcaaaaa cggtagcgta agcggcgaag
     8101 gcatgaccaa caacggtcgt ggcgctctgc gtcagaacgg cgacggtgtc ggcggttcta
     8161 tcacttatga ttacgaaggc ttcggtatcg gtgctgcagt ttccagctcc aaacgtactg
     8221 atgatcaaaa tggtagctac atcagcaatg gtgtagttcg taactacatc ggtactggcg
     8281 accgtgctga aacctacact ggtggtctga aatacgacgc taacaacatc tacctggctg
     8341 ctcagtacac ccagacctac aacgcaactc gcgtaggttc cctgggttgg gcgaacaaag
     8401 cacagaactt cgaagctgtt gctcagtacc agttcgactt cggtctgcgt ccgtctgtag
     8461 catacctgca gtctaaaggt aaaaacctgg gtgtcatcaa tagtcgtaac tacgacgacg
     8521 aagatatcct gaaatatgtt gatgttggcg cgacctacta cttcaacaaa aacatgtcca
     8581 cctatgttga ctacaaaatc aacctgctgg acgacaacca gttcactcgt gacgctggca
     8641 tcaacactga taacatcgta gctctgggtc tggtttacca gttctaatct cgattgatat
     8701 cgaacaaagg gcctgcgggc ccttttttca ttgttttcag cgtacaaact cagttttttg
     8761 gtgtactctt gcgaccgttc gcatgaggat aatcacgtat ggaaataagc tttacccgcg
     8821 tggcactgct ggctgccgcg ctcttctttg ttggttgcga tcaaaaacca caacccgcca
     8881 aaacccacgc tactgaagtt accgttcttg aaggcaaaac catgggtacc ttctggcgtg
     8941 ccagcatccc gggcattgat gccaaacgca gtgccgaact taaagaaaag attcagaccc
     9001 agctggacgc cgacgatcag ctgctttcga cctataaaaa agattccgcg ctgatgcgct
     9061 ttaacgactc gcaaagtttg tcgccgtggc cagtaagtga agcgatggcc gaaatcgtca
     9121 ctacctcgct gcgcattggc gcgaagaccg atggtgcgat ggatattacc gtcgggccgc
     9181 tggtaaatct gtggggcttt ggcccggaac aacagccggt tcaaattccg agccaggaac
     9241 agatcgacga gatgaaagcc aaaactggtt tacagcacct tacggtcatt aatcagtcgc
     9301 atcagcaata tctgcaaaaa gacctgccgg atttatatgt cgatctctct accgtcggcg
     9361 aaggttatgc ggcggatcac ttggcacgct tgatggagca ggaagggatt tcccgctatc
     9421 tggtgtcggt gggcggcgcg ctgaacagcc gtggtatcaa cggtgaaggc ctgccgtggc
     9481 gggtagcgat tcaaaaacca accgataaag aaaacgcagt tcaggctgtg gtggatatca
     9541 acggtcatgg gattagcacc tctggcagct accgtaacta ttacgaactg gacggcaaac
     9601 gtctttccca tgttatcgat ccgcaaaccg ggcgtcccat cgaacacaat ctggtatccg
     9661 tgacggtgat tgctccgacg gcgctggaag ccgatgcctg ggatactggc ttgatggtac
     9721 tcgggccgga gaaagccaaa gaggttgttc gccgggaagg gctggcggtt tatatgatca
     9781 ccaaagaagg cgatagcttt aaaacctgga tgtcaccaca gtttaaaagc ttccttgtca
     9841 gcgaaaaaaa ttaaagcgca agattgttgg tttttgcgtg atggtgaccg ggcagactaa
     9901 aggctatcct taaccaggga gctgattatg aaaaacgcca catgcttaac tgacgatcaa
     9961 cgctggcaat ctgtcttagc ccgcgacccg aatgccgacg gcgaattcgt tttcgccgtg
    10021 cgcaccacag gcatcttttg ccgtccgtct tgccgcgcca gacatgcctt gcgggaaaat
    10081 gtctccttct acgcaaatgc cagcgaggca ctcgccgccg gatttcgccc ctgcaaacgc
    10141 tgccaacctg ataaagccaa tgcccagcaa catcggttgg ataaaatcac ccacgcgtgt
    10201 cgactgctgg aacaggaaac gcctgtaacg ttggaagcct tagccgacca ggtggcgatg
    10261 agtccatttc atctacatcg gttgtttaaa gcgactaccg gaatgacgcc taaagcctgg
    10321 caacaggcct ggcgcgctcg ccgtttgcgc gaatcgctgg cgaaagggga gagcgtgacg
    10381 acgtctattc ttaacgccgg attccccgac agcagcagtt actaccgcaa agccgatgaa
    10441 acgctgggca tgacggctaa acaattccgt cacggtggcg aaaatctggc ggtgcgttac
    10501 gcgctggctg attgtgcgct gggtcgttgc ctggtggcag aaagcgagcg ggggatttgc
    10561 gcgatattgc tgggcgatga tgacgcgaca ttaatcagcg agttgcagca gatgtttccc
    10621 gctgccgaca acgcgcctgc cgatctgacg tttcagcaac atgtgcgtga agtgatcgcc
    10681 agcctcaatc aacgcgatac gccgctgacg ttaccgctgg atattcgcgg cactgctttt
    10741 cagcaacaag tctggcaggc actgcgcacg ataccttgcg gtgaaaccgt actggctaac
    10801 gccatcggca aaccgaaagc ggtacgggcc gttgccagcg cctgtgccgc caacaagctg
    10861 gctatcgtaa taccctgtca tcgagtggtc ggtggtgatg gcacactttc cggttaccgc
    10921 tggggcgtgt ctcgtaaagc gcaactgctg cgccgcgaag ctgaaaatga ggagaggtaa
    10981 tgttggatct gtttgccgat gctgaaccgt ggcaagagcc actggcggct ggtgcggtaa
    11041 ttttacggcg ttttgctttt aacgctgcgg agcaactgat ccgcgatatt aatgacgttg
    11101 ccagccagtc gccgtttcgc cagatggtca cccccggggg atataccatg tcggtggcga
    11161 tgaccaactg tgggcatctg ggctggacga tccatcggca aggttatctc tattcgccca
    11221 ttgatccgca aacaaataaa ccgtggcccg ccatgccaca aagctttcat gatttatgtc
    11281 aacgtgcggc tacggcggcg ggctatccag atttccagcc agatgcttgt cttatcaacc
    11341 gctacgctcc tggcgcgaaa ctgtcgctgc atcaggataa agacgaaccg gatctgcgcg
    11401 cgccaattgt ttctgtttct ctgggcttac ccgcgatttt tcaatttggt ggcctgaaac
    11461 gtaatgatcc gctcaaacgt ttgttgttgg aacatggcga tgtggtggta tggggcggtg
    11521 aatcgcggct gttttatcac ggtattcaac cgttgaaagc ggggttttat ccactcacca
    11581 ccgactgccg ctacaacctg acattccgtc aggcaggtaa aaaagaataa aaataagaat
    11641 tattattgct gtgcgcgcga agatgtttaa actgcgggct gtaattcatt gtccgggttt
    11701 tctgcatgga acttcttgta cttgtctggc ggcagtatcg ctggccattt atcagtgtga
    11761 tggcgctaag cctcgccagt gcggcattag gcattggctt aattgctttt atcaatcagc
    11821 gccttatcga aatggcggat accagtctgc tggtattgcc ggagtttctg ggattattgc
    11881 tgctgttgat ggcagtcact ctcggatcgc aactggcgct caccactttg gggcatcact
    11941 tcgtttaccg actgcgtagt gaatttatca agcggattct ggatacccac gtcgagcgca
    12001 ttgaacaact cggtagcgcc tcgttgttgg cgggattaac cagcgatgtg cgcaatatca
    12061 ccattgcttt tgtgcgtctt ccggaactgg tgcaggggat cattctcact atcggttccg
    12121 cggcgtatct gtggatgctg tcgggcaaaa tgttgctggt aacggctatc tggatggcga
    12181 tcaccatctg gggtggtttt gtactggtgg cgcgggtgta caaacatatg gcgaccctac
    12241 gtgaaaccga agacaagctg tacacggatt ttcaaactgt acttgaaggg cgcaaagagc
    12301 tgactctgaa ccgggaacgc gccgagtatg tgtttaacaa cctctacatc cccgatgcgc
    12361 aagagtatcg ccatcatatt attcgcgcag acaccttcca tcttagtgcc gtgaactggt
    12421 caaacatcat gatgctgggc gctatcggcc tggtgttctg gatggcgaac agtctcggtt
    12481 gggctgatac caacgttgct gcgacctatt cgttgacgct tttattcctg cgtacgccgc
    12541 tgctttcggc ggttggcgca ttgccgacgc tgctgatggc gcaggtggcg tttaacaagc
    12601 tgaacaaatt cgcgctcgcg cctttcaaag cagagtttcc gcgcccgcag gcgtttccca
    12661 actggcaaac gctggagctg cgtaatgtga cgtttagcta tcaggataac gcgttttccg
    12721 ttggtccgat taatctcacc atcaaacgtg gcgagctact gtttctgatt ggtggtaacg
    12781 gtagtggaaa atcgacgctg gcgatgttgt tgacgggctt gtatcagcca caaagcggcg
    12841 aaatcttgct ggatggcaaa cctgtcagcg gcgaacaacc ggaagattat cgcaaactgt
    12901 tttcggcagt gtttaccgat gtctggcttt ttgatcaact gctggggccg gagggtaaac
    12961 ccgctaaccc gcaactggtt gagaagtggc tggcgcagct gaaaatggct cataagcttg
    13021 agttaagcaa cgggcgtatt gttaacctga agttatcaaa agggcagaaa aaacgcgtgg
    13081 cgctgttgct ggcgctggca gaagaacgcg atattatcct gctggatgaa tgggcggcgg
    13141 atcaggatcc acacttccgt cgtgagtttt atcaggtgtt gctgccgctg atgcaggaga
    13201 tgggtaaaac tattttcgct atcagtcatg atgatcatta ctttatccac gccgaccgcc
    13261 tgctggaaat gcgcaatggg caacttagcg agctgacggg cgaagagcgc gatgccgctt
    13321 cgcgtgatgc cgttgcccgg acggcataat attacgacgc tgacctggcg gactgctgcc
    13381 gtcaggtcaa tatgctaaaa atcccttcat ttctaataac cctataatta atcaacgaaa
    13441 ttataatgtt tctaaaatta gaatataatt tataaacatt atttaaatgt tgttacttaa
    13501 gtgttacctt tgatgccgcg caaatctcac ctgcaataaa gcgactaaaa gtaaggcatt
    13561 aacaagatga aaaaagtgac tgccatgctc ttctcgatgg ccgtggggct taatgccgtt
    13621 tcgatggcgg caaaagcgaa agcgtccgag gagcaggaaa ctgatgtact gttgattggc
    13681 ggcggcatta tgagcgccac gttggggacc tatttacgcg agctggagcc tgaatggtcg
    13741 atgaccatgc tggagcgcct ggagggtgtc gcgcaggaga gttcgaacgg ctggaataac
    13801 gccggaaccg ggcattctgc actgatggaa ctgaactaca ccccgcaaaa cgccgatggc
    13861 agtatcagta ttgaaaaagc agtcgccatt aacgaagcat ttcagatttc ccgccagttc
    13921 tgggcgcacc aggtcgagcg cggcgtgctg cgtactccgc gttcatttat caataccgtt
    13981 ccgcatatga gctttgtctg gggcgaggat aacgttaatt tcctgcgcgc ccgttacgcc
    14041 gcgttgcaac aaagctcgct gtttcgcggt atgcgttact ctgaagatca cgcgcagatc
    14101 aaagagtggg caccgttagt gatggaaggg cgcgatccgc aacagaaagt ggcagccacg
    14161 cgtacggaaa ttggtaccga tgtgaactac ggcgagatca cccgccagtt aattgcttcc
    14221 ttgcagaaga aatctaactt ctcgctgcaa ctcagcagcg aagtccgcgc cctaaagcgt
    14281 aatgacgata acacctggac cgttaccgtt gccgatctga aaaatggcac tgcacagaac
    14341 attcgtgcca aatttgtctt tatcggcgcg ggcggtgcgg cgctgaagct gttacaggaa
    14401 tcggggattc cggaagcgaa agactacgcc ggtttcccgg tgggcggaca gttccttgtt
    14461 tcggaaaacc cggacgtggt taatcaccat ctggcgaagg tttacggtaa agcatccgtt
    14521 ggcgcaccac cgatgtcggt tccgcatatc gatacccgcg ttctggacgg taaacgcgta
    14581 gtgctgtttg ggccatttgc caccttctca accaaattcc tcaaaaacgg ttcattgtgg
    14641 gatctgatga gttccaccac cacctctaac gtgatgccga tgatgcacgt cgggctggat
    14701 aatttcgatc tggtgaaata tctggtgagt caggtgatgt tgagtgaaga ggatcgtttt
    14761 gaagcgttga aagagtacta tccgcaggcg aaaaaagagg actggcgttt gtggcaagcg
    14821 gggcagcgtg tgcagattat caagcgtgat gccgataaag gtggtgtact gcgtctgggt
    14881 actgaagtgg tcagtgacca gcaagggacc attgccgcgc tcctgggagc atcgccaggg
    14941 gcgtcaaccg ccgcgccgat tatgttggat ctgctggaaa aagtatttgg cgatcgcgtt
    15001 tccagcccgc aatggcaggc tacgttgaaa gcgatcgttc cgtcttatgg acgcaagctg
    15061 aacggtgatg tagcggcaac agaacgcgag ttgcagtaca ccagcgaagt gctggggctg
    15121 aaatacgaca agccgcaggc agcagatagt acgccaaaac cgcagttgaa accgcaaccc
    15181 gttcaaaaag aagtggcgga tattgcgttg taatgatgtg ccacatccgg catggtatgc
    15241 cggatgtggc gtatactgat aagacgcgcc agcgtcgcat caggcaaccg gctcgggcgt
    15301 tagatgttgg atgcggcgta accgccttat ccaaccaacg gggtgccgta cttcacaaca
    15361 gtttagcgaa cttctgcgtt gtcaattttc tcttccgcct tccagacgcg gtatttcaca
    15421 tctacattgt ctggcgtata caccacgatc ggcagcttgc tgttgtaacg cagcattcca
    15481 gcatcgccca gatacgcggt gacaaatttc ttctctttct tgccatccgg gcaggccatc
    15541 atcgtggaaa ccggcgagct aactttatca aagacataat aatcatagcc ccagccttcc
    15601 agcgttttgc tttccagctt cccgccgaga cgatgcaaat tgcaatcgac ttccagcgtc
    15661 tgaccgatta acagttctac tttcagggta gattcatctt cttgcggggt taactgaatc
    15721 acctgacgct tcatcccttt ttcagcttgt ggataaggcg cgattttttc cagtggctgg
    15781 acgctttctg ccgcccaggc ggaagtggta gcgaaagcgg caaacaatac tgcaggtaga
    15841 atggtcttca tttttttgct ttccttcttc atttataggt tttcaccgat gatgtcgctg
    15901 aaggttaaca tattcataag atttattaat ttgctttatg aataacttaa agaatattca
    15961 gacaatttta cctgctcttt tagtgttaaa ttctgatgag agaacaaacc agcagaaata
    16021 tctctggtga attaatcgct atataaatat gtttataacc atttgaaatg tgagcaaaag
    16081 cccgtttttg ccacgcttcc cgccactctt ttgatcctgc tacaggtttt accccgatcg
    16141 gggtatgcat ctttgacaca tcctttaata tcttagcggt tataaaaatg gcttattaat
    16201 tatgcggttt atttggtcgc tctcaatttt cagagcgcgt taatgatgga aggtcaatgt
    16261 gaagattgat gcatcccgtc ggggcatact cactggtcgc tggcgcaaag ccagtaacgg
    16321 tatccgtccg ccctggtccg gtgatgaatc tcattttctg acccattgca cccgttgcga
    16381 cgcctgtatt aatgcttgtg aaaacaacat tctgcaacgc ggcgcgggcg gctatccgag
    16441 cgttaatttc aaaaataacg agtgcagctt ctgttacgct tgcgcgcagg cttgccccga
    16501 atcgctattt tctccgcgcc acaccagggc ctgggatttg cagttcacca ttggggacgc
    16561 gtgcctggcg tatcagtcag ttgaatgccg ccgctgtcag gatagctgtg agccaatggc
    16621 aattatcttt cgcccgacgc tctccgggat ctaccagccg caacttaata gccaactttg
    16681 caacggatgt ggagcatgtg cagccagctg tccggtatca gccataactg cggagtatct
    16741 ccatgcacac taactggcaa gtttgcagcc tggtcgtgca ggccaaaagc gaacgaattt
    16801 cagacatcag cacccaactg aacgcctttc ccggctgtga agttgctgtc agcgacgcgc
    16861 cgagcggtca gttgattgtg gtggtggaag cagaagacag cgaaacgctg atccaaacca
    16921 ttgagtcagt acgcaacgta gagggcgtgc tggcggtgtc gctggtttat caccagcagg
    16981 aagagcaagg tgaggaaaca ccatgaaact cagtcgtcgt agctttatga aagctaacgc
    17041 cgttgcggcc gctgcggcgg ctgccggtct cagcgtgccg ggcgttgccc gcgccgttgt
    17101 tggtcagcag gaagccatca aatgggataa agcgccgtgc cgtttctgcg gtactggttg
    17161 cggcgttctg gtcggaacgc agcagggacg tgtggtggcc tgtcagggcg acccagacgc
    17221 accggttaac cgtggcctga actgcattaa gggctatttc ctgcccaaga tcatgtacgg
    17281 taaagaccgt ttgacgcagc cgctgctgcg tatgaaaaac ggtaaatatg acaaagaagg
    17341 cgaatttacc ctaatcacct gggatcaggc cttcgatgtg atggaagaga aattcaaaac
    17401 cgccctgaaa gaaaaagggc cggaatcgat cggtatgttc ggttctggtc agtggactat
    17461 ctgggaaggt tatgccgcgt ccaagctgtt taaagcgggc ttccgttcga acaacatcga
    17521 cccgaacgcg cgtcactgta tggcgtcggc agtagttggc tttatgcgta cctttggtat
    17581 ggatgagccg atgggctgct atgacgacat cgagcaggct gacgcgtttg tgctgtgggg
    17641 cgcaaacatg gcggagatgc acccgatcct ctggtcgcgc atcactaacc gtcgtctctc
    17701 taatcagaac gtcaccgtgg cggtgctttc tacctaccag catcgtagct tcgagctggc
    17761 ggataacggc atcatcttta cgccacagtc tgacctggtg atcctgaact acatcgccaa
    17821 ctatatcatt caaaacaatg cgataaatca ggacttcttc agcaaacacg ttaacctgcg
    17881 caaaggggcg acggacattg gctacggttt acgtccgacc catccgctgg aaaaagcagc
    17941 gaagaatcca ggttctgacg cctccgaacc gatgagcttt gaagattaca aagccttcgt
    18001 tgccgagtat acgctggaaa aaactgccga aatgaccggc gtgccgaaag accagttaga
    18061 acaactggcg cagctgtatg ccgatccgaa caagaaagtc atctcctact ggacgatggg
    18121 cttcaaccag catactcgtg gcgtgtgggc taacaacctg gtctacaacc tgcacctgct
    18181 gaccggcaaa atttcccagc cgggttgcgg tccgttctcc ctgaccgggc aaccttctgc
    18241 gtgtggtact gcgcgtgaag tgggcacctt tgctcaccgt ctgcctgcgg acatggtggt
    18301 gaccaacgag aaacaccgtg atatctgcga gaagaagtgg aatatcccga gcggcaccat
    18361 tccggcgaaa atcggtctgc acgcggtggc acaagaccgt gcgctgaaag acggcaaact
    18421 gaatgtttac tggaccatgt gtaccaacaa catgcaggcc gggccgaaca tcaatgaaga
    18481 gcgtatgccg ggctggcgcg atccgcgcaa cttcatcatc gtctccgatc cgtatccgac
    18541 agtcagtgcg ctggccgccg acttgatcct gccgaccgca atgtgggtag agaaagaggg
    18601 cgcttacggt aacgccgaac gccgtactca gttctggcgt caacaggtac aggcaccggg
    18661 cgaagcgaaa tcggatctct ggcagttagt ccagttctcc cgccgcttca aaactgaaga
    18721 agtatggccg gaagatctgc tggcgaagaa accggaactg cgtggcaaaa cgctgtacga
    18781 agtgctgtat gccacaccgg aagtgagcaa attcccggta tccgaactgg cggaagatca
    18841 gctgaacgat gaatcccgcg agctgggctt ctatctgcaa aaagggctgt tcgaagagta
    18901 cgcatggttt ggtcgcggtc acggtcacga tctggcaccg ttcgatgact accacaaagc
    18961 gcgcggtctg cgctggccgg tggtgaacgg taaagaaacg cagtggcgtt acagcgaagg
    19021 taacgacccg tacgtgaaag cgggcgaagg ctataagttc tacggcaaac cggatggcaa
    19081 agcggtgatc ttcgcactgc cattcgaacc ggcggcggaa gcaccggatg aagagtacga
    19141 cctgtggctc tctaccggac gcgttctgga gcactggcac accggcagta tgactcgccg
    19201 tgtaccagaa ctgcaccgcg ccttcccgga agcggtcctg tttattcacc cgctggatgc
    19261 gaaagcgcgc gatctgcgtc gtggcgacaa agtgaaagtg gtttctcgcc gtggcgaagt
    19321 gatctcgatt gtcgaaacgc gcggtcgtaa ccgtccgccg cagggcctgg tgtacatgcc
    19381 gttcttcgac gccgcacagc tggttaacaa actaacgcag gacgcgaccg atccgctctc
    19441 gaaagagacg gacttcaaga agtgcgcagt caaactggag aaggtgtaag ccattatgtc
    19501 ccggtcagcg aaacctcaaa atggtcgccg ccgctttctg cgcgatgttg ttcgcacagc
    19561 aggcgggctg gctgccgtgg gtgtggcgct ggggttacaa cagcaaaccg cacgcgcatc
    19621 tggcgtgcgg ttgcgcccgc ccggagccat aaacgagaac gcctttgcca gtgcctgtgt
    19681 gcgttgcggt cagtgtgttc aggcttgccc ttacgacacc ttaaaactgg cgacgctggc
    19741 ctctggtctg tcggcgggca cgccatattt tgtcgcacgg gatattcctt gcgaaatgtg
    19801 tgaggacatt ccgtgcgcca aagtgtgccc gagcggtgcg ctggatcgtg agatcgaatc
    19861 gatcgacgac gcgcggatgg ggctggcggt actggtggac caggaaaact gtctcaactt
    19921 tcaggggctg cgctgcgatg tttgttatcg cgaatgcccg aaaattgatg aggccatcac
    19981 cctggagctg gagcgcaaca cgcgtaccgg taagcacgcc cgctttctgc cgacggttca
    20041 cagagacgcc tgtaccggtt gcggtaagtg cgaaaaagtg tgcgtgctgg aacaaccggc
    20101 aatcaaggtg ttaccgctgt cactggctaa aggggagtta ggtcaccatt accgcttcgg
    20161 ctggctggag gggaacaatg gcaaatcgta aacgtgacgc cgggcgcgag gcgctggaga
    20221 aaaaaggctg gtggcgcagt caccgttggc tggtgttacg tcgcctttgt cagttcttcg
    20281 tgctggggat gtttttgagc ggtccgtggt ttggtgtgtg gatcttacac ggtaactaca
    20341 gcagtagcct gttattcgac accgttccgc tgaccgatcc gctgatgacg ctgcaaagtc
    20401 ttgccagcgg tcatctgcct gccacggtgg cgttgaccgg tgcggtgatt atcaccgtgc
    20461 tctacgccct ggcaggaaag cgattatttt gcagctgggt ctgcccgctg aatccgatta
    20521 ccgacttagc gaactggtta cgcaggcgct ttgacctcaa tcagtctgcg acgatccctc
    20581 gccacattcg ctacgtgctg ttggtggtca ttctgctggg atcggcctta actggcacgc
    20641 tcatctggga atggatcaac cccgtatctc tgatggggcg cagcctggta gtgggcttcg
    20701 gcagcggcgc gctgcttatt ctcgcgctgt ttttatttga tctactggtc gttgaacacg
    20761 gctggtgcgg gcacatttgt ccggttggcg cgctgtatgg cgtgctgggt agcaaaggtg
    20821 tgattaccgt tgcggccacc gatcggcaga aatgtaaccg ctgtatggat tgttttcatg
    20881 tttgcccgga accgcgtgtg ctacgtgccc cggtgctgga tgaacaaagc ccggtgcagg
    20941 tcaccagccg cgattgcatg acgtgcggtc gctgcgtgga tgtctgttct gaggatgtat
    21001 ttacaataac tacacgatgg agttcgggag cgaaatcatg aaaagccatg acctgaagaa
    21061 agcgctgtgt caatggacgg cgatgctggc cctggtggta agcggcgcgg tttgggcggc
    21121 taatggcgtc gattttagcc agtcgccgga agtatccggg acacaggaag gggccattcg
    21181 gatgccaaaa gagcaggaac ggatgccgct gaactatgtg aatcagccgc caatgatccc
    21241 gcatagcgtt gaaggttatc aggtaacgac caataccaac cgctgcttgc agtgccacgg
    21301 tgtcgaaagt tatcgcacca ctggcgcgcc gcgcattagt cctactcact ttatggacag
    21361 cgacggcaaa gtgggcgcgg aagtggctcc gcgtcgttat ttctgtctgc aatgtcacgt
    21421 accgcaggcc gataccgcgc caatcgtggg gaataccttt accccatcaa aaggttacgg
    21481 gaaataagag gttattatgg gaaattctga ccgtaagcct ggtctgatta agcgcctgtg
    21541 gaaatggtgg cgtaccccca gccgtctggc gctggggacg ctgctgttga tcggttttgt
    21601 tggcggcatc gtcttctggg gtggctttaa caccgggatg gaaaaagcca ataccgaaga
    21661 gttctgcatt agctgccacg aaatgcgcaa cacggtatat caggaataca tggattccgt
    21721 gcactacaac aaccgtagcg gcgtccgtgc gacctgtccg gattgtcacg ttccgcacga
    21781 gtttgtgccg aagatgatac gcaagctcaa agcaagtaaa gagctgtatg gtaaaatttt
    21841 tggcgttatt gacacgccgc agaaatttga agctcatcgt ctgacgatgg cacagaatga
    21901 gtggcggcgc atgaaggaca ataactcgca ggagtgccgt aactgtcaca acttcgagta
    21961 tatggataca accgcccaga aatcggttgc cgcgaagatg catgaccagg cggtgaaaga
    22021 tgggcaaacc tgtattgatt gccataaagg gatagcgcac aagctgcccg atatgcgtga
    22081 agtcgagcca ggtttttaac agggttattg cgtgggtatg cttgaagcca gagagttact
    22141 ttgtgagcag gatgaacgaa ccttatttag tggcttgtca tttacgctga acgcaggaga
    22201 gtgggtacaa atcaccggta gcaacggcgc ggggaagaca acgcttctcc gtttgctgac
    22261 ggggttgtct cgccctgacg caggcgaggt tctctggcaa gggcagccct tgcatcaggt
    22321 acgcgacagc taccatcaaa acctgttatg gataggccat cagccgggga tcaaaacccg
    22381 gctgacggcg ttagaaaatc tgcactttta tcatcgcgat ggcgataccg cacaatgtct
    22441 ggaagccctg gcgcaggccg ggcttgccgg attcgaagat attcctgtaa atcagctctc
    22501 ggccgggcaa caacgccgcg tcgctttagc gcgtctgtgg ctgacccgtg ccacgttatg
    22561 gatcctcgac gagcctttta ccgcgattga cgttaacggc gtcgatcgtc tgacccagcg
    22621 tatggcgcag catacggagc agggggggat tgtgattctg actacccacc agccgctcaa
    22681 cgttgctgaa agtaaaattc gccgcatttc actgacgcaa acgagggccg catgatgttc
    22741 tggcgcattt tccgtcttga gctgcgtgta gcgtttcgcc atagcgccga aatcgccaac
    22801 ccgctgtggt tcttcctgat tgtaattacc ctttttccgc tcagtatcgg tccggagccg
    22861 caactgctgg cgcgtattgc accgggcatt atctgggttg ctgcgctgct ttcatccttg
    22921 ctggcgctgg aacgactgtt ccgtgacgat ttgcaggacg gcagtcttga acaattgatg
    22981 ttgttgccgt tacccttgcc cgccgttgtg ctggcgaagg tgatggcgca ctggatggta
    23041 accggtctgc cgttactcat cctttcgcca ctggtagcaa tgctactggg aatggatgtt
    23101 tatggctggc aagtgatggc gctgacgctg ctgctgggaa cgcctacgct tggctttctc
    23161 ggtgcaccgg gtgtggcgtt gacagtggga cttaagcgcg gtggtgtgct gctcagcata
    23221 ctggtgttac cgctgactat cccattactc atctttgcca ccgccgcgat ggacgcggct
    23281 tccatgcatt tgcccgttga cgggtatctg gcaattttag gcgcgttgct ggcaggcacc
    23341 gcgacattaa gtccttttgc gacggcggca gcgttacgaa tcagcattca ataacgctgg
    23401 attactgatt tttgttcgag tctggtatcg aaactatgtg gaaaacactg catcaactgg
    23461 cgatcccacc acggctgtat caaatctgtg gctggtttat accgtggctg gcaattgcca
    23521 gtgtggtcgt gcttaccgtc ggctggatct ggggattcgg ctttgctccg gctgattatc
    23581 agcagggaaa tagctaccgc attatctacc tgcatgtgcc tgcggcgatc tggtcgatgg
    23641 gtatttatgc atcaatggcg gtggcggcgt ttattggcct tgtctggcag atgaaaatgg
    23701 ccaacctggc ggtggcggcg atggccccca ttggtgccgt gtttaccttt attgccctgg
    23761 ttaccggctc tgcatgggga aaaccgatgt ggggcacctg gtgggtatgg gatgcacgtc
    23821 tgacttctga actggtgctg ctgtttttgt atgtgggtgt gattgccctg tggcacgcct
    23881 tcgacgaccg ccgtctggcg ggccgtgcgg caggtatcct ggtgctgatt ggcgtggtga
    23941 atctgccgat tattcattac tccgtggagt ggtggaacac cctgcatcag ggatcaacgc
    24001 ggatgcagca aagtatcgat ccggcgatgc gttcgccgct gcgctggtcg atttttggct
    24061 tcctgctcct gtctgccacg ctgacgctga tgcggatgcg taatttgatt ttgctgatgg
    24121 aaaaacgccg tccgtgggtg agtgaactga tactgaaaag aggccgtaaa tgacccttgc
    24181 atttgcttcc tggaatgaat ttttcgcaat gggcggttac gccttttttg tctggctggc
    24241 ggtggtgatg accgttattc cgctggtggt tttggtcgtg cactcggtga tgcagcatcg
    24301 cgcaattctg tgtggtgtgg agcaacagcg ggcgcgtgag gcgcgtttac gtgctgcgca
    24361 acagcaggag gctgcatgaa tattcgccgt aaaaaccgct tgtggattgc ctgtgctgtg
    24421 ttggcaggtt tggcgctgac catcggtctg gtgctgtatg cgctgcgctc gaatatcgat
    24481 ctcttttata cgccggggga aattctctac ggcaagcgtg aaactcagca aatgccggaa
    24541 gtcggtcagc gtctgcgcgt tggcgggatg gtgatgccgg gtagtgtgca gcgcgatccc
    24601 aattcgctga aagtgacctt caccatttac gacgctgaag gctcagtgga tgtctcttac
    24661 gaaggcattt tgccggatct gttccgtgaa gggcagggcg ttgtggtgca gggcgagctg
    24721 gaaaaaggca atcatatcct cgcgaaagaa gtgctggcga aacacgacga aaactacacg
    24781 ccgccagaag ttgagaaagc gatggaagct aaccaccgtc gcccggcgag tgtttataag
    24841 gacccagcat catgatgccg gaaattggaa acgggctgct gtgcctggcg ctgggaattg
    24901 cgctgctgct gtccgtgtat ccgctatggg gcgtggcgcg cggagatgcg cgcatgatgg
    24961 cgtcttcccg cttgtttgcc tggctgctgt ttatgtctgt ggctggcgca tttctggtgc
    25021 tggtcaatgc tttcgtggtc aacgacttta ccgtcaccta tgttgccagc aactccaata
    25081 cccagcttcc ggtgtggtat cgcgtggcgg ctacctgggg cgcgcatgaa ggttcgcttc
    25141 tgctgtgggt gctgctgatg agcggctgga cctttgcggt ggcgattttt agtcagcgta
    25201 ttccgctgga tattgtggcc cgtgtactgg cgataatggg gatggtcagc gtcggctttt
    25261 tgctgttcat tctctttacc tctaacccgt tctcacgcac gctgccgaac ttcccaattg
    25321 aagggcgcga tcttaacccg ctgttacagg atccggggct gatcttccat ccgcctctgc
    25381 tttatatggg gtacgtgggt ttctcggtgg cgtttgcttt tgccattgct tctttgctga
    25441 gcgggcgtct ggacagcact tatgcgcgtt ttactcgccc gtggacgctg gcggcgtgga
    25501 ttttcctgac gctcggcatt gtgctcggtt ccgcatgggc ctattacgaa ctcggctggg
    25561 ggggctggtg gttctgggat ccggtagaaa acgcctcgtt tatgccgtgg ctggtgggga
    25621 ctgcgctgat gcactcactg gcggtcactg aacaacgcgc cagcttcaaa gcgtggacat
    25681 tactgctggc aatcagtgcc ttctcgttgt gtctgctggg gactttcctg gtgcgttccg
    25741 gcgtgctggt atcggtacac gcgtttgcct ctgatccggc acgcggtatg tttatcctcg
    25801 cctttatggt actggtgatt ggtggttcgc tgctgctgtt tgccgcgcgt ggacacaaag
    25861 ttcgctcacg cgtaaacaat gcgctgtggt cgcgggaatc tctgctgtta gcgaacaacg
    25921 ttttgttggt cgccgcgatg ctggtggtat tactggggac gctgctgccg ctggtgcaca
    25981 agcaactggg actgggcagt atttcgattg gcgaaccgtt cttcaacacc atgtttacct
    26041 ggctgatggt gccgtttgcg ctgctgcttg gtgtcggtcc tctggtgcgc tgggggcgcg
    26101 atcgcccacg taaaatccgc aatttattga ttatcgcctt catctcaacg ctggtgctgt
    26161 cgctgctgct gccgtggctg ttcgaaagca aagttgtggc gatgacggtg ctcggcctgg
    26221 caatggcctg ctggattgct gtgctggcaa ttgcggaagc tgcgctgcgt atttcacgtg
    26281 gcacgaaaac caccttcagt tattggggaa tggtggcggc tcacctgggg ctggcagtga
    26341 caattgttgg tattgccttt agccagaact atagcgttga gcgtgatgtg cgcatgaagt
    26401 ccggcgatag cgtcgatatt catgaatatc gcttcacctt ccgtgatgtc aaagaggtga
    26461 ctggcccgaa ctggcgcggc ggtgtggcga ctattggcgt aacgcgagag ggtaaaccgg
    26521 aaacggtgct gtatgcggaa aaacgttatt acaacactgc cgggtcgatg atgaccgaag
    26581 cggcgattga cggcggcatc acgcgtgacc tgtacgccgc gctcggtgaa gagctggaaa
    26641 acggcgcgtg ggccgtgcgt ctttactaca aaccgtttgt tcgctggatt tgggcgggcg
    26701 ggctgatgat ggcgttgggc ggactgctgt gtctgtttga tcctcgctat cgtaagcgcg
    26761 tgagtccgca aaaaactgcg ccggaggctg tatgaagcgc aaagtattgt taattccgtt
    26821 gattatcttc ctggcgattg ccgcggcgct gctgtggcag ctggcgcgta atgccgaagg
    26881 ggatgatccg accaatctgg aatcggcgct cattggcaag ccagtgccga agtttcgcct
    26941 cgaatcactg gacaatccgg ggcagtttta tcaggcggat gtgctgactc agggcaaacc
    27001 tgtgctgctt aacgtttggg ctacctggtg tccgacctgc cgtgcggaac atcaatatct
    27061 gaatcagctt tctgcgcagg gcattcgcgt ggtcggcatg aactataaag acgatcgcca
    27121 gaaggcaata agctggctga aagagctggg caatccttac gcgctaagcc tgtttgatgg
    27181 cgatggcatg ttagggctgg atctcggtgt ctatggcgcg ccagaaacgt tcctcatcga
    27241 cggcaacggc atcattcgct atcgccatgc gggcgatctc aatcctcgcg tctgggaaga
    27301 agagatcaag ccgctgtggg agaaatacag taaggaggcc gcacaatgag gtttttattg
    27361 ggcgtgctga tgctgatgat ctccggctca gcgctggcga ccatcgacgt gttgcagttt
    27421 aaggatgaag cgcaggagca gcagttccgc caactcactg aagaactacg ctgcccgaaa
    27481 tgccagaaca acagcattgc cgattccaac tcgatgattg ccaccgacct gcgccagaaa
    27541 gtgtatgaac tgatgcagga aggtaaaagt aaaaaagaga ttgtcgatta tatggtggcg
    27601 cgttacggca acttcgtcac ttacgatccg ccgttaacgc cgctgaccgt gctgctgtgg
    27661 gtgcttccgg tagtggctat tggcattggc ggttgggtca tttacgcccg ttcgcggcgt
    27721 cgggtacgcg tggtgccgga cgcgtttcct gaacaaagcg tgccggaagg taagcgtgcc
    27781 ggatatattg tttatctgcc gggtattgtg gtggcgttaa ttgtggctgg cgtcagctac
    27841 taccagactg gcaattatca gcaggtgaaa atctggcagc aggccacggc acaggctccg
    27901 gcgttgctgg acagggcgct ggatccgaaa gccgatccgc tcaacgaaga agagatgtcg
    27961 cgtcttgcgc tggggatgcg tactcaactg caaaaaaatc cgggagatat agaaggctgg
    28021 attatgttgg gccgcgttgg catggcgctg ggtaacgcca gtatcgccac cgatgcatac
    28081 gctactgcgt atcgcctcga tccgaaaaac agtgatgccg cactgggtta tgctgaagcg
    28141 ttgacacgtt catctgatcc caacgacaac cgcctcggtg gtgaactgct gcgccagttg
    28201 gtgagaacgg accatagcaa tatccgtgtg ttaagcatgt atgcgtttaa tgcctttgag
    28261 cagcagcgat ttggcgaagc cgttgccgcg tgggagatga tgttgaaact cttatctgcc
    28321 aacgatactc gccgtgcggt gattgaacgt agtatcgcgc aggcgatgca acatttgtcg
    28381 ccgcaggaga gtaaataaaa agaggcccga tggtgatgcc atcgggctat ttttattgtg
    28441 ccccgcgttg ttgcaggaac agaatggtgg ctgccacgcg tgagcggaca ttgagtttgc
    28501 gcagcagatt gcgaatatgt acttttactg tctgctctga aatattcaac accgaggcaa
    28561 tctgtttatt tgacagcccc tgtgccagct cgtgcagaac atccagctcg cgctccgtca
    28621 gcacgctgaa gggatcttct tccgcgccaa acatttcacg ttcacgtaag tactgattga
    28681 cgcgttcgct aaagactttg ctgcctttcg ctccagcacg aattgcttcc agcaatactt
    28741 ccgggtcgct gtctttcaac agataaccgt ctgcgcctgc gtctatcagc gcaaagacat
    28801 cgctggaggc atctgatacg gtcaggataa taatttgcgc ggtaacgcca tccctgcgca
    28861 aggcattgag ggtatccagg ccactcatac ctttcatatt gagatccagc aagatcacgt
    28921 cgatatccag tctatttgcc agatcgatcg cgctcgcgcc gtcgcccgct tcggcgacca
    28981 cttcaaagct aggatcaagc tccagtaact gacgaacacc gcgtcgcata agtggatgat
    29041 catccacaat catcacctga aaaggcgttg cttcaggcat agtagtctcc tggggtttta
    29101 ttagaatcat tatgtttgag cgtagcgttg gtaacgctac caggcgcgtg aatgttaccc
    29161 cattttatgt atggacatgt agtgaaaact acaaccttgt cgttcgcttt ggcatcattt
    29221 tcattataaa gacggaattt tgcatcagct tctc
//
LOCUS       DAJVUJ010000032        29058 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.032, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000032 DAJVUJ010000000
VERSION     DAJVUJ010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29058
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.032"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            162..605
                     /locus_tag="OQQ48_001340"
     CDS             162..605
                     /locus_tag="OQQ48_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004997737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCS1952174.1"
                     /translation="MDLALVTGQREGDLAAMRWEDIRDGRLYVEQQKTGAKIRISLPT
                     TISRLNLTLTDVLDNLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFH
                     EIRSLSGRLHSAEKGSDFTQALLGHRSSSMTDKYRDGRGREWKDI"
     gene            841..1659
                     /gene="ybhA"
                     /locus_tag="OQQ48_001341"
     CDS             841..1659
                     /gene="ybhA"
                     /locus_tag="OQQ48_001341"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HCS1952175.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDMPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(1660..2718)
                     /gene="modC"
                     /locus_tag="OQQ48_001342"
     CDS             complement(1660..2718)
                     /gene="modC"
                     /locus_tag="OQQ48_001342"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HCS1952176.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(2721..3410)
                     /gene="modB"
                     /locus_tag="OQQ48_001343"
     CDS             complement(2721..3410)
                     /gene="modB"
                     /locus_tag="OQQ48_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HCS1952177.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(3410..4183)
                     /gene="modA"
                     /locus_tag="OQQ48_001344"
     CDS             complement(3410..4183)
                     /gene="modA"
                     /locus_tag="OQQ48_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCS1952178.1"
                     /translation="MARKWLNLFAGAALSFAVAGNTLADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFIIDSTTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVVSKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(4349..4498)
                     /gene="acrZ"
                     /locus_tag="OQQ48_001345"
     CDS             complement(4349..4498)
                     /gene="acrZ"
                     /locus_tag="OQQ48_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HCS1952179.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            4627..5415
                     /gene="modE"
                     /locus_tag="OQQ48_001346"
     CDS             4627..5415
                     /gene="modE"
                     /locus_tag="OQQ48_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HCS1952180.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNANNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            5483..6955
                     /gene="modF"
                     /locus_tag="OQQ48_001347"
     CDS             5483..6955
                     /gene="modF"
                     /locus_tag="OQQ48_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HCS1952181.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKILLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLVTGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQHLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPNGGLYRYVLTKIY"
     gene            7216..8232
                     /gene="galE"
                     /locus_tag="OQQ48_001348"
     CDS             7216..8232
                     /gene="galE"
                     /locus_tag="OQQ48_001348"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HCS1952182.1"
                     /translation="MRVLLTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGIGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            8242..9288
                     /gene="galT"
                     /locus_tag="OQQ48_001349"
     CDS             8242..9288
                     /gene="galT"
                     /locus_tag="OQQ48_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HCS1952183.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNHDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            9292..10440
                     /gene="galK"
                     /locus_tag="OQQ48_001350"
     CDS             9292..10440
                     /gene="galK"
                     /locus_tag="OQQ48_001350"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HCS1952184.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLAEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            10434..11474
                     /gene="galM"
                     /locus_tag="OQQ48_001351"
     CDS             10434..11474
                     /gene="galM"
                     /locus_tag="OQQ48_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HCS1952185.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            11676..12428
                     /gene="gpmA"
                     /locus_tag="OQQ48_001352"
     CDS             11676..12428
                     /gene="gpmA"
                     /locus_tag="OQQ48_001352"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCS1952186.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(12586..13638)
                     /gene="aroG"
                     /locus_tag="OQQ48_001353"
     CDS             complement(12586..13638)
                     /gene="aroG"
                     /locus_tag="OQQ48_001353"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HCS1952187.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            13954..14334
                     /locus_tag="OQQ48_001354"
     CDS             13954..14334
                     /locus_tag="OQQ48_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HCS1952188.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAATDAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            14448..15389
                     /gene="zitB"
                     /locus_tag="OQQ48_001355"
     CDS             14448..15389
                     /gene="zitB"
                     /locus_tag="OQQ48_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HCS1952189.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSIVVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHAHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(15386..16105)
                     /gene="pnuC"
                     /locus_tag="OQQ48_001356"
     CDS             complement(15386..16105)
                     /gene="pnuC"
                     /locus_tag="OQQ48_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HCS1952190.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMLLMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSCALSH"
     gene            complement(16143..17186)
                     /gene="nadA"
                     /locus_tag="OQQ48_001357"
     CDS             complement(16143..17186)
                     /gene="nadA"
                     /locus_tag="OQQ48_001357"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HCS1952191.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(17435..17510)
                     /locus_tag="OQQ48_001358"
     tRNA            complement(17435..17510)
                     /locus_tag="OQQ48_001358"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17475..17477),aa:Lys,seq:ttt)
     gene            complement(17657..17732)
                     /locus_tag="OQQ48_001359"
     tRNA            complement(17657..17732)
                     /locus_tag="OQQ48_001359"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17697..17699),aa:Lys,seq:ttt)
     gene            complement(17736..17811)
                     /locus_tag="OQQ48_001360"
     tRNA            complement(17736..17811)
                     /locus_tag="OQQ48_001360"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17776..17778),aa:Val,seq:tac)
     gene            complement(17863..17938)
                     /locus_tag="OQQ48_001361"
     tRNA            complement(17863..17938)
                     /locus_tag="OQQ48_001361"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17903..17905),aa:Lys,seq:ttt)
     gene            complement(17941..18016)
                     /locus_tag="OQQ48_001362"
     tRNA            complement(17941..18016)
                     /locus_tag="OQQ48_001362"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17981..17983),aa:Val,seq:tac)
     gene            complement(18052..18127)
                     /locus_tag="OQQ48_001363"
     tRNA            complement(18052..18127)
                     /locus_tag="OQQ48_001363"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18092..18094),aa:Lys,seq:ttt)
     gene            complement(18292..19083)
                     /gene="cpoB"
                     /locus_tag="OQQ48_001364"
     CDS             complement(18292..19083)
                     /gene="cpoB"
                     /locus_tag="OQQ48_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HCS1952192.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(19093..19614)
                     /gene="pal"
                     /locus_tag="OQQ48_001365"
     CDS             complement(19093..19614)
                     /gene="pal"
                     /locus_tag="OQQ48_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HCS1952193.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(19649..20941)
                     /gene="tolB"
                     /locus_tag="OQQ48_001366"
     CDS             complement(19649..20941)
                     /gene="tolB"
                     /locus_tag="OQQ48_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HCS1952194.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPSAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(21074..22255)
                     /gene="tolA"
                     /locus_tag="OQQ48_001367"
     CDS             complement(21074..22255)
                     /gene="tolA"
                     /locus_tag="OQQ48_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HCS1952195.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAQKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEKAK
                     AEAEKKAAAEKAAADKKAAADKKAAEKAAAEKAAADKKAAAEKAAAEADDIFGELSSG
                     KNAPKTGGGAKGNNASPAGSGNSKNNGASGADINNYAGQIKSAIESKFYDASSYAGKT
                     CTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKIPKPPSQAVYEVFKNAPLDFK
                     P"
     gene            complement(22320..22748)
                     /gene="tolR"
                     /locus_tag="OQQ48_001368"
     CDS             complement(22320..22748)
                     /gene="tolR"
                     /locus_tag="OQQ48_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HCS1952196.1"
                     /translation="MARARGRGRRELKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(22752..23444)
                     /gene="tolQ"
                     /locus_tag="OQQ48_001369"
     CDS             complement(22752..23444)
                     /gene="tolQ"
                     /locus_tag="OQQ48_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HCS1952197.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(23441..23845)
                     /gene="ybgC"
                     /locus_tag="OQQ48_001370"
     CDS             complement(23441..23845)
                     /gene="ybgC"
                     /locus_tag="OQQ48_001370"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCS1952198.1"
                     /translation="MNTTLFRWPARVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(23995..24288)
                     /gene="ybgE"
                     /locus_tag="OQQ48_001371"
     CDS             complement(23995..24288)
                     /gene="ybgE"
                     /locus_tag="OQQ48_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HCS1952199.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIIGLIFFFF"
     gene            complement(24288..24401)
                     /gene="cydX"
                     /locus_tag="OQQ48_001372"
     CDS             complement(24288..24401)
                     /gene="cydX"
                     /locus_tag="OQQ48_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HCS1952200.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(24416..25555)
                     /gene="cydB"
                     /locus_tag="OQQ48_001373"
     CDS             complement(24416..25555)
                     /gene="cydB"
                     /locus_tag="OQQ48_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCS1952201.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(25571..27139)
                     /gene="cydA"
                     /locus_tag="OQQ48_001374"
     CDS             complement(25571..27139)
                     /gene="cydA"
                     /locus_tag="OQQ48_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HCS1952202.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            complement(27987..>29058)
                     /locus_tag="OQQ48_001375"
     CDS             complement(27987..>29058)
                     /locus_tag="OQQ48_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HCS1952203.1"
                     /translation="IEESSDDGDEYDYSPAKEEWVITAANAKPQCDIIHEAWQSRAVI
                     RYDMAVPLNLSERSARQSTGRVGVVLVVTLSHNSRRIDVDINLDNQADDHRLRVLVPT
                     PFNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYVALQEGRNGMAV
                     FSEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPGRPSGIKMPVPDSQLRGLLS
                     CRLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMP
                     PVGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEEN
                     QGSNLSGPFLPGQSRTFSYRLA"
BASE COUNT         7342 a         7638 c         7085 g         6993 t
ORIGIN      
        1 acatttttaa aacagctata gcttctggtc ttgccgaaga agatccagct tctgcaacca
       61 ggccattcag aactgaagtg aagcgcagtc gactgttaat tgacgagtat ctgttaatca
      121 ggaaaatagc agacgtccag aacgaatggt tcggcctttg catggatctt gcactggtaa
      181 caggacagcg cgaaggagat ctggcggcta tgagatggga ggatataaga gatggcagac
      241 tatacgtgga acaacaaaaa acaggggcaa aaattcgcat ttctctcccc accacgattt
      301 ccagattgaa tttaacactt accgatgtac tggataactt gaaaaaaata aacggtaaaa
      361 acgaaaagtt actcggaggg aagacagcca ggacaatcgc agcacagttc aggattgccc
      421 gagacacatc cggattgaaa tgggaaggtg acccaccgcc atttcatgaa atccgaagtt
      481 tatccggtcg cctgcactca gcagaaaaag gcagtgattt cactcaggcg ctccttggac
      541 atcgttcatc cagcatgacg gataaatacc gtgacggacg agggagggaa tggaaagaca
      601 tttagaatta atttcgaatg ttttcgaaac accacttgta attaattgat tataaaggat
      661 taattcctaa gaggcatttg cttcatgaat gctcctttgc atttagcaac tgactgtaac
      721 agctaaaatt agtcgctttt ggcggtgaat gctgaattaa aacgcagaat tttgtccgat
      781 agtggtatcg gtgtaccatt cgcgcagacg aaaattctta accttaatct ggaatacgcc
      841 atgaccacac gcgtgattgc tctcgactta gacggcacct tattgacccc gaaaaagacc
      901 ctgcttcctt catcgataga agccctggcc cgcgctcgcg aagcaggcta tcaattaatc
      961 atcgtcacag gtcgccatca cgtcgctatt catccttttt atcaggcgct ggcgctggat
     1021 acacctgcta tttgctgtaa tggcacctat ttgtatgatt atcatgcaaa aaccgtgctg
     1081 gaagcggacc caatgcccgt taataaagcc ctgcaactca ttgagatgct gaatgaacac
     1141 cacattcacg gtctgatgta tgtggatgat gcaatggtct atgagcaccc gaccgggcat
     1201 gtcattcgca cgtctaactg ggcgcaaacc ctgccgccgg aacagcgtcc gactttcaca
     1261 caagtcgctt ctctggctga aacggcgcaa caagttaacg ccgtatggaa gttcgccctg
     1321 acgcacgatg acatgccgca attgcagcat tttggtaagc atgtcgaaca tgaactggga
     1381 ctggagtgtg aatggtcctg gcacgatcag gttgatattg cacgcggcgg caacagcaaa
     1441 ggtaaacgtt tgacgaaatg ggttgaggcg caaggctggt cgatggaaaa cgtcgtggcg
     1501 ttcggcgata actttaatga tatcagtatg ctggaggccg ctggtacagg cgtggcgatg
     1561 ggcaacgccg atgacgcggt aaaagcgcgc gccaacattg tgattggtga taacaccacc
     1621 gacagcattg cccagttcat ttatagccac ctgatttaat caggcggtta tcgacacact
     1681 tttaatttgc gcgtacagcc acaggcctgg tttgatcgcc agttcatccc tggcccacgg
     1741 gctgatacgc gcccacagcg ttttaccgcc gacttccagc tccacttcca cctgaccgtt
     1801 gtcgtcataa ctattaacaa cttttgcccg caatacgtta cgaatgctgg tttgctgcgg
     1861 cggttgtaaa accagagaaa catcggaagc ctgaatgcgg atacgtagcg cagcttgcag
     1921 cggttcgtcc agcttattga cccacaaatg ctgatcgccc agcgccagcg cggtcatcgc
     1981 gtaatgcgga tgatgctcaa gcaccgtcac tttcagaatg ctactttgtt gctctttcgg
     2041 cagccacgga ttcatcacgc tactacccca gacttcttcc agtgcgccaa aggctttcac
     2101 ctgaccgttt tccagtacca tcactctgtc tgccagatgg aggatctcat ccagcgaatg
     2161 gctgacatac aacatcggga tgttgatttc ccgcgtcaga cgttgcagat aaggcaacag
     2221 ttcgcgttta cgcggaatat ccagtgacgc cagcggttca tccagcaaca gcaattctgg
     2281 tgccgtcagc aaagcccgcc caatcgctac gcgctgtttt tcgcctccgg acaggctgcc
     2341 tggtaaacgg tcaagcaacg gttcaatgcc taaaagcgcc accagcttat cgaactgatc
     2401 gaccatgctt ttcgccatgc cgtagcgcag attgccacgc actttgtaat gcggaaacag
     2461 ccgcgcatcc tgaaaaacat agccaacgcg acgcttttct ggcgtcaggc agataccttt
     2521 ttcggcatca tttagtaccc gaccattgag gacaatcctc cctttttgcg ggcgcgtcag
     2581 tccactgatg gcgttaatca gcgaagtttt tccggcaccg gagacgccaa agatagcagt
     2641 gatgccattg gcgggcagcg tttcattaat agtcaggcaa tggttgccca acgtctggga
     2701 aaaattcagt tccagcatga ttagcgcccc gcccgttcac ggctgattct ggccagccat
     2761 tctgaaatca acagggagat catcgccagc gcaatagaaa taatgcacag tctcgccgcg
     2821 ccactttccc cgccgggggt ctggatcagg gtatacatgg cagagggaat ggttcgcgtt
     2881 tcgccaggaa tgttcgacac aaaggtgatg gttgcaccaa actcaccgag agaacgagca
     2941 aaagccagta ccgtaccaac aataattccc ggtaaggtca gcggtaacgt gatagtaaag
     3001 aaaacgcgcc agcgcccggc acctagtgtt cttgcggcct gttcgagttt gacatcaacc
     3061 ccttccagcg ccagacgaat tgcccgcacc atcagcggaa acgacatgac ggcggcagcg
     3121 agaaccgcgc cgcgccagct aaaggcgaag gtaataccaa accagtcata cagacgttca
     3181 ccgataaatc cgcgccgtcc catcgaaact aataataagt aaccgacgac cacgggcggt
     3241 aacaccagcg gtagatgcag tacgctgtcg agcagagctt tgcccggaaa cgtgcaacgc
     3301 accagtaacc aggcaaaaaa gatcccaaac ggcaggctaa acagcacagc cagggaagaa
     3361 actttcaggc ttaataaaac tgcctgccat tctggatcgg tcagtatcat tacttggttg
     3421 taaatccgta acgtttaaag atttccgctg cctgcggtcc cttcagataa tcataaaatg
     3481 ctttcactgt cgcattgtta tgcccttcca caacagcaac cggatattcc acttttttat
     3541 gtgaatcttc cgggaaggtg gcaaccactt ttaccccttt gctgacaact gcgtcagaac
     3601 cgtaaacaat gcccaacggc gcttcattgc gttcgaccaa cgccagcgcg ccacgaacat
     3661 cttccgccgg agccaatttc ggggagagcg tatcccatgc gcccagtttt tgcagtgctt
     3721 cttttgcata aatgccagca gggacatgtt ccggatcgcc aaccgcgagg cgaccgccat
     3781 tcagcagcga agtccagtta gttgtgctgt cgatgatgaa atctttctgc tcgctggctt
     3841 tcggtgctac aacgaccagg ctattgccga gcagtgtctg gcgcgaagcc gtatcgatcg
     3901 cttttttatc aaccgcataa tccatccatt tctgatcggc agagataaac agatccgcag
     3961 gcgctcccgc ttcaatctga cgggccagag tagatgacga agcgaaagaa gaaaccacat
     4021 ccacgccttt ttctttttta tactgcgtag caatgtcctg cattgcgtta gtcagtgatg
     4081 cggcggcgaa caccgtgatt ttcccttcat ctgccagtgt attgccagca acagcgaaag
     4141 agagtgccgc cccggcaaac aagttcaacc atttacgagc cattggtaac cccttaatgt
     4201 aacgttatat aggtgacaat ataacggcta actcagaatt ttcccagcag ttattgctaa
     4261 cctttgtgag gtagataaga aaaaatatcg gctggaaaag caggaagttg agaaaaagaa
     4321 atgcccgact aagtgggcat tcagggaatc aatgattttg tccgggctgg tcttttttac
     4381 caacaccaga aaagatgttg aatacttcac caagaccgta aatcagaccc aggatgatgg
     4441 ccatcacgac aggtaccatg attacggcga ataccagact ttttaataac tctaacatgg
     4501 tcaactccag atatagtcat gagactattc taaccgctaa gcacagaaaa gcactcccct
     4561 tttgtgcggt cagctttgcg tggcgttcgt tttccgtcac aataagactt ttgccaggac
     4621 attgttatgc aggccgaaat ccttctcacc cttaagctcc aacaaaaatt attcgccgac
     4681 ccgcgccgga tttcgctact aaaacacatt gcgctttccg gttccattag ccagggagcg
     4741 aaagatgccg gtatcagcta taaaagcgcc tgggatgcca ttaacgagat gaatcagtta
     4801 agcgagcata ttctggttga gcgcgcaaca ggcggtaaag gtggcggcgg cgcagtactg
     4861 acccgctatg gtcagcgact gattcagctc tatgacttac tggcgcaaat ccagcaaaaa
     4921 gcctttgatg tgttaagtga cgatgacgcc ctgccgctga acagcctgct ggccgcgatc
     4981 tcacgttttt cactgcaaac cagcgcccgt aaccagtggt tcggtaccat caccgcccgc
     5041 gatcatgatg acgttcaaca gcatgttgat gtcttactgg ctgacggaaa aacacgcctg
     5101 aaagtcgcaa ttaccgcaca aagcggcgca cgtctggggc tggatgaagg caaagaagtg
     5161 ttgatattgc taaaagcgcc gtgggtaggc attactcagg acgaagcggt cgcacaaaac
     5221 gccaacaacc aattaccggg tattattagt catattgagc gcggcgcaga gcagtgcgaa
     5281 gtattaatgg cgctacccga cgggcaaaca ctgtgcgcca cagtgccggt aaatgaagcg
     5341 acttctcttc agcaagggca gaatgtcacg gcctacttta atgccgacag cgtgattatc
     5401 gccacgctgt gctaagcgtg ttgacaattt gttatgaaac acgtatccct gtcagtaatc
     5461 gctgcacaaa atgggatata aaatgtcatc gttgcaaatt ttgcaaggca cgtttcgtct
     5521 tagcgacaca aaaacgctgc aattgcctca gctaacgtta aacgcgggtg atagttgggc
     5581 gtttgtcggt tcgaatggaa gcgggaaatc ggccctggcc cgcgcgctgg cgggggaact
     5641 tccgcttttg aaaggtgaac ggcaaagcca gttttcccac atcactcgtc tctccttcga
     5701 gcaattgcaa aaactcgtca gcgacgaatg gcagcggaat aacaccgata tgctcggccc
     5761 tggcgaagat gacaccggac gcactacggc tgagatcatt caggatgaag taaaggatgc
     5821 accgcgttgc atgcaactgg cgcagcagtt cggtattacc gccctcctcg accgacgctt
     5881 taaatacctt tccactggcg agacgcgaaa aatcctgctg tgtcaggcgc tgatgtcgga
     5941 gcctgacttg ttgattcttg atgagccgtt cgatggcctg gatgttgcct cacgtcagca
     6001 gctggctgag cgactcgcct cgttacatca gtccggtatt actctggtac tggtgctcaa
     6061 tcgcttcgat gagatcccgg agtttgtcca gtttgctggc gtgctggcgg attgcacgtt
     6121 agcggaaact ggcgctaaag aggaactgct ccaacaagca ctcgtcgcgc aactggcaca
     6181 tagtgaacag cttgaaggtg tgcaactgcc ggagccggat gaaccttcag cacgtcacgc
     6241 cttacccgcc aacgaaccgc gcattgtgct gaacaatggc gtggtttctt ataacgatcg
     6301 ccccattctt aataacctta gctggcaggt gaatccaggt gaacactggc aaattgtcgg
     6361 gccaaatggt gcaggaaaat cgacgttatt aagcctggtt actggcgatc atccgcaagg
     6421 ttacagcaac gatttgacgc ttttcggacg acgtcgcggc agcggcgaaa ccatctggga
     6481 tatcaaaaag catatcggtt acgtcagcag tagtttgcat ctggattacc gggtcagcac
     6541 taccgtgcgt aatgtgattc tttctggcta ttttgattcg attggcattt atcaggccgt
     6601 ttcggatcgc cagcaaaaac tggtgcagca gtggctggat attctcggca ttgataaacg
     6661 tacggctgac gctccgttcc atagtctttc ctggggacag cagcatctgg cgctgatcgt
     6721 ccgcgcactg gtgaaacatc cgacgttgct tattctcgat gaaccactac aggggcttga
     6781 tccgctcaat cgccagctta tccgccgttt tgttgatgtg ctgattagcg aaggtgaaac
     6841 gcaattgttg tttgtttcgc accacgctga agatgcgcct gcctgtatta cccatcgcct
     6901 tgagttcgtg ccgaacggtg gactctatcg ctatgtgctg acaaaaatat attgagtcgg
     6961 tagtgctgac cttgccggag gcggcctaag cgccctctcc ggccaacggt tcgacgcatg
     7021 caggcatgaa accgcttctt ttttcagata aaaagcgcaa tcattcataa accctctgtt
     7081 ttataatcac ttaatcgcgc ataaaaaacg gctaaattct tgtgtaaacg attccactaa
     7141 tttattccat gtcacacttt tcgcatcttt gttatgctat ggttatttca taccataagc
     7201 ctaatggagc gaattatgag agttctgctt accggtggta gcggttacat tggaagtcat
     7261 acctgcgtgc aattactgca aaacggtcat gatgtcatca ttcttgataa cctctgtaac
     7321 agtaagcgca gcgtactgcc tgttatcgag cgtttaggcg gcaaacatcc aacgtttgtt
     7381 gaaggcgata ttcgtaacga agcgttgatg accgagatcc tgcacgatca cgctatcgac
     7441 accgtgatcc acttcgccgg gctgaaagcc gttggcgaat cggtacaaaa accgctggaa
     7501 tattacgaca acaatgtcaa cggtactctg cgcctgatta gcgccatgcg cgccgctaac
     7561 gtcaaaaact ttatttttag ctcctccgcc accgtttatg gcgatcagcc caaaattcca
     7621 tacgttgaaa gcttcccgac cggcacaccg caaagccctt acggcaaaag caagctgatg
     7681 gtggaacaga tcctcaccga tctgcaaaaa gcccagccgg actggagcat tgccctgctg
     7741 cgctacttca acccggttgg cgcgcatccg tcgggcgata tgggcgaaga tccgcaaggc
     7801 attccgaata acctgatgcc atacatcgcc caggttgctg taggccgtcg cgactcgctg
     7861 gcgatttttg gtaacgatta tccgaccgaa gatggtactg gcgtacgcga ttacatccac
     7921 gtaatggatc tggcggacgg tcacgtcgtg gcgatggaaa aactggcgaa caagccaggc
     7981 gtacacatct acaacctcgg cgctggcata ggcagcagcg tgctggacgt ggttaatgcc
     8041 ttcagcaaag cctgcggcaa accggttaac tatcattttg caccgcgtcg cgagggcgac
     8101 cttccggcct actgggcgga cgccagcaaa gccgaccgtg aactgaactg gcgcgtaacg
     8161 cgcacactcg atgaaatggc gcaggacacc tggcactggc agtcacgcca tccacaggga
     8221 tatcccgatt aaggaacgac catgacgcaa tttaatcccg ttgatcatcc acatcgccgc
     8281 tacaacccgc tcaccgggca atggattctg gtttcaccgc accgcgctaa gcgcccctgg
     8341 cagggggcgc aggaaacgcc agccaaacag gtgttacctg cgcacgatcc agattgcttc
     8401 ctctgcgcag gtaatgtgcg ggtgacaggc gataaaaacc acgattacac cgggacttac
     8461 gttttcacta atgactttgc ggccttgatg tctgacacgc cagatgcgcc agaaagcaac
     8521 gatcctttga tgcgttgcca gagcgcgcgc ggtaccagcc gggtgatctg cttttcaccg
     8581 gatcacagta aaacgttgcc agaactgagc gttgcggcat tgacggaaat cgtcaaaacc
     8641 tggcaggagc aaaccgcaga gctgggaaaa acgtacccgt gggtgcaggt ctttgaaaac
     8701 aaaggtgcgg cgatgggctg ctctaacccg catccgcacg gacagatttg ggcaaatagc
     8761 ttcctgccta acgaagctga gcgcgaagac cgcctgcaaa aagaatattt tgccgaacag
     8821 aaatcaccaa tgctggtgga ttatgttcag cgcgagctgg cagacggtag ccgtaccgtt
     8881 gtcgaaaccg aacactggtt agccgttgtg ccttactggg ctgcctggcc gttcgaaacg
     8941 ctactgctgc ccaaagccca cgttttgcgg atcaccgatt tgaccgacgc ccagcgcagc
     9001 gatttggcgc tggcgttgaa aaagctgacc agtcgttatg acaacctctt ccagtgctcc
     9061 ttcccctact ctatgggctg gcacggcgca ccatttaatg gcgaagagaa tcaacactgg
     9121 cagctgcacg cgcactttta tccgcctctg ctgcgctccg ccaccgtacg taaatttatg
     9181 gttggttatg aaatgctggc agagacccag cgagacctga ccgcagaaca ggcagcagag
     9241 cgtttgcgcg cagtcagcga tatccatttt cgcgaatccg gagtgtaaga aatgagtctg
     9301 aaagaaaaaa cacaatctct gtttgccaac gcatttggct accctgccac tcacaccatt
     9361 caggcgcctg gccgcgtgaa tttgattggt gaacacacgg actacaacga cggtttcgtt
     9421 ctgccctgcg cgattgatta tcaaaccgtg atcagctgtg caccacgcga tgaccgtaaa
     9481 gttcgcgtga tggcagccga ttatgaaaat cagctcgacg agttttccct caatgcgccc
     9541 attgtcgcgc atgaaaacta tcaatgggcg aactacgttc gtggcgtggt gaaacatctg
     9601 caactgcgta acaacagctt cggcggtgtg gacatggtga tcagcggcaa tgtgccgcag
     9661 ggtgccgggt taagttcttc cgcttcactg gaagtcgcgg tcggaaccgt attgcagcag
     9721 ctttatcatc tgccgctgga cggcgcacaa atcgcgctta acggtcagga agcagaaaac
     9781 cagtttgttg gctgtaactg cgggatcatg gatcagctaa tttccgcact cggcaagaaa
     9841 gatcatgcct tgctgattga ctgtcgttca ctggggacca aagcagtttc catgccgaaa
     9901 ggtgtggctg tcgtcatcat caacagtaac ttcaaacgta ccctggttgg cagcgaatac
     9961 aacacccgtc gtgaacagtg cgaaaccggt gcgcgtttct tccagcagcc agccctgcgc
    10021 gatgtcacca ttgaagagtt caacgctgtt gcacatgagc tggacccaat cgtggcgaaa
    10081 cgcgtgcggc atatcctgac tgaaaacgcc cgcaccgttg aagctgccag cgcgctggag
    10141 cagggcgacc tgaaacgtat gggcgagttg atggcggagt ctcatgcctc tatgcgcgat
    10201 gatttcgaaa tcaccgtgcc gcaaattgac actctggcag aaatcgtcaa agctgtgatt
    10261 ggcgacaaag gtggcgtacg catgaccggc ggcggatttg gcggctgtat cgtcgcgttg
    10321 atcccggaag agctggtgcc tgccgtacag caagctgtcg ctgaacaata tgaagcaaaa
    10381 acaggtatta aagagacttt ttacgtttgt aaaccatcac aaggagcagg acagtgctga
    10441 acgaaactcc cgcactggca cccgatggtc agccgtaccg actgttaact ttgcgtaaca
    10501 acgcagggat ggtagtcacg ctgatggact ggggtgcgac tttactttca gcccgtattc
    10561 cgctttccga tggcagcgtc cgcgaggcgc tgctcggctg tgccagcccg gaatgctatc
    10621 aggatcaggc cgcgtttctg ggggcctcta ttggtcgtta tgccaaccgc atcgccaata
    10681 gccgttatac ctttgacggt gaaaccgtga ccctttcgcc aagtcagggc gttaaccagc
    10741 tgcacggcgg cccggaaggg ttcgacaaac gtcgctggca gattgtgaac cagaacgatc
    10801 gtcaggtgct gtttgccctg agttcagatg atggcgatca gggcttcccg ggtaatctcg
    10861 gcgcaacggt gcaatatcgt ctgaccgacg ataaccgtat ctccattact tatcgcgcca
    10921 cagttgataa accttgcccg gtgaatatga ctaatcacgt ctatttcaat cttgacggcg
    10981 agcagtctga cgtgcgcaat cacaagttgc agattctggc ggacgaatat ctgccggttg
    11041 atgaaggcgg cattccgcac gacggcctga aatctgtcgc cggaacgtct tttgatttcc
    11101 gcagcgccaa aatcatcgcc agtgagtttc ttgccgacga cgatcagcgc aaagtgaaag
    11161 gttacgatca cgcattcttg ttacaggcca aaggcgatgg caagaaagtg gcggcgcatg
    11221 tctggtcagc agatgaaaaa ttgcagctga aggtctacac caccgctccg gctctgcaat
    11281 tctactccgg caacttcctc ggcggcacac cgtcgcgggg aacagaacct tacgccgact
    11341 ggcaagggct ggcgttggaa agcgagtttc tgccggacag cccgaaccac cctgaatggc
    11401 cgcaaccgga ctgcttcctg cgtcctggcg aagagtattc cagcctgacg gaatatcagt
    11461 ttattgctga gtaatgttaa gccctcgtca tgagggcttt atctcatatt gctcaaatca
    11521 ccagcaaaca ccgacatatt tgcaactcaa tattcacaac aaccttacac tgcgccacta
    11581 ttttcgctat ggttatgcgt aagcattgct gttgcttcgt cgcggcaata taatgagaat
    11641 tattatcatt aaaagatgat ttgaggagta agtatatggc tgtaactaag ctggttctgg
    11701 ttcgtcatgg cgaaagtcag tggaacaaag aaaaccgttt caccggttgg tacgacgtgg
    11761 atctgtctga gaaaggcgta agcgaagcaa aagcagcagg taagctgctg aaagaggaag
    11821 gttacagctt tgactttgct tacacttctg tgctgaaacg cgctatccat accctgtgga
    11881 atgtgctgga cgaactggat caggcatggc tgcccgttga gaaatcctgg aaactgaacg
    11941 aacgtcacta cggtgcgttg cagggtctga acaaagcgga aactgctgaa aagtatggcg
    12001 acgagcaggt gaaacagtgg cgtcgtggtt ttgcagtgac tccgccggaa ctgactaaag
    12061 atgatgagcg ttatccgggt cacgatccgc gttacgcgaa actgagcgag aaagaactgc
    12121 cgctgacgga aagcctggcg ctgaccattg accgcgtgat cccttactgg aatgaaacta
    12181 ttctaccgcg tatgaagagc ggtgagcgcg taatcatcgc cgcacacggt aactccctgc
    12241 gtgcgctggt gaaatatctt gataacatga gcgaagaaga gattcttgag ctgaatatcc
    12301 cgactggcgt gccgctggtg tatgagttcg acgagaattt caaaccgctg aaacgctatt
    12361 atctgggtaa tgctgacgag atcgcagcga aagcagcggc ggttgcaaac cagggtaaag
    12421 cgaagtaaac gtcattcgtt taaaatgaga aagccgactg caagtgagtc ggcttttttg
    12481 ttgctaacaa tggagcacat tgcctgatgc gacgcttcgc gacttatcag gcctgtggtg
    12541 attcatcgga tacgccactc tgacggcgca tccgacaatt aaaccttacc cgcgacgcgc
    12601 ttttactgca ttcgccagtt gacgtaacag agcatcggta tcttcccagc cgatgcaggc
    12661 atcggtgatg ctcttaccgt aggccagcgg ctccccgctc tcgaggctct gattgccttc
    12721 caccagatgg ctttccacca tcacgccaat aatggccttt tcgccaccgg caatctgctg
    12781 gcaaacgtca gcacaaacat ccatctgctt tttgaattgt ttggacgagt tagcatggct
    12841 gaaatcgatc atcacctgtg ctggcaggcc tgctttgttc agcccttctt tcacttcagc
    12901 aacgtgcttc gcgctgtagt taggctcttt accgccgcgc agaatgatat ggcaatcgcc
    12961 gttaccgctg gtattcacaa tcgccgaatg cccccatttc gttacggaca ggaagcagtg
    13021 cggcgcaccg gcggcattaa tggcatcgat agccacttta atcgtaccgt cggtgccatt
    13081 tttgaagccg actggacaag aaagccctga tgccagttcg cggtgcacct gcgattcggt
    13141 ggtacgtgcg ccaattgcgc cccagctcat caggtcagcg agatattgtg gggtgatcat
    13201 atcgagaaac tcacccgccg ctggcagacc gctgtcgtta atatcaagca gcaatttacg
    13261 ggctatacga agaccgtcgt tgatctggaa gctgttatcc atatgcggat cgttaatcag
    13321 ccctttccag cccaccgtgg tacgcggctt ttcaaaatag acgcgcatta cgatttccag
    13381 ctcatctttc agctcttcac gcagcgccag caagcgagtg gcatactctt tagccgcgac
    13441 aggatcatga attgagcacg ggccaatcac caccaacagg cgatcatcat tacctttcag
    13501 gatcttatgg atcgcttttc gggcatgggc aaccgtattc gcggcatttt cagtagcggg
    13561 gaatttttcc agcaatgcga caggaggaag taactctttg atttctttga tgcgtaaatc
    13621 gtcgttctga taattcatgt ctgttccagt gttgccatac ttatcttagt gaatgcaata
    13681 cttccaatct atatcttccg tcagaatgtg taaacggggt tttacattat gaacggatta
    13741 atcctggaat tagcaagaaa aacgccagat tgtcgcgaaa aacgagatct ctcctacaat
    13801 ttctaactgt aactcctttg tttgttaatt atttcaagat tctctgctgc gtttcataaa
    13861 ctggctgaaa tcttaaacca atgccttata ttcacctgca aatgcactgt tggaagaggt
    13921 tatccgacat aacgaccata acaggagcat cctatgaaaa tgacaaaact ggccacatta
    13981 tttctgactg ccactctaag ccttgccagc ggtgccgcac tggctgccga tagcggagcg
    14041 caaactaata acggccaggc aaacgccgca actgatgcgg gccaggtagc ccctgacgcc
    14101 cgtgaaaatg tcgcgccaaa taacgtcgac aataacgggg taaataccgg ttctggcggc
    14161 acaatgctgc attcggatgg ttcttcaatg aacaatgacg gaatgaccaa agatgaagag
    14221 cacaagaaca ccatgtgcaa agatggtcga tgtccggata tcaataaaaa ggtacaaacc
    14281 ggtgatggca tcaataatga tgtcgatacc aaaaccgacg gtaccacgca gtaaccctgc
    14341 aaaatagcca ataatgggag agcggagtct ctccctttta atttttcact tctaaaagca
    14401 gtatgatgtc ataaagttaa gtgagaataa ttaagggatg actcgtcatg gcgcactcac
    14461 actcacacac gtcctcacac ctgccagaag ataataatgc tcgtcgcttg ttgtatgctt
    14521 tcggcgtgac tgccgggttt atgctggtag aagtcgttgg tggttttctt tctggttctc
    14581 tggcattgct ggccgatgcg ggtcatatgt tgaccgatac tgccgccctg cttttcgccc
    14641 tactcgccgt gcaattttca cgtcgtcctc ccaccattcg ccacactttc ggttggctaa
    14701 gactgaccac gctcgcggcg tttgtgaatg ccatcgccct cgtggtaatt accattttga
    14761 ttgtctggga ggcgatagaa cgcttccgca cgccgcgtcc ggtcgagggc ggcatgatga
    14821 tggcaattgc cgtggctggg ttgctggcaa atatactttc tttctggtta cttcatcacg
    14881 gcagcgaaga gaaaaacctc aacgtacgag cagcggcatt gcatgtgctc ggggacctgt
    14941 tgggttcggt tggggctatt attgccgcgt tgattattat ctggaccggc tggacacctg
    15001 ctgaccccat tctctcgata gtggtgtcgc ttctggttct gcgtagcgcg tggcgactgt
    15061 tgaaagatag cgtgaatgaa ttacttgaag gtgcaccggt atcgctggat atcgctgaac
    15121 tgaagcgtcg tatgtgccgg gaaatcccgg aagtccgcaa tgtgcaccat gtacatgtat
    15181 ggatggtagg tgagaagccg gtgatgacgc tgcatgtgca ggtgatcccg ccacacgctc
    15241 acgatgcctt gttggatcag atccaacact acctgatgga tcactatcag attgagcacg
    15301 ccaccattca gatggaatat caaccttgtc atgggccaga ctgccacctg aatgaagggg
    15361 tgtctggcca ttcacatcat caccattaat gggacagcgc gcatgagcct ctttcacgtg
    15421 cgctgttgat ccacatccga ctgccgttga gcgcaataaa ggtcaggatg atgtactcca
    15481 gagacatggc gtaaacgccc tgaagtgcaa aaataacgac gctaatcacg ttaataatca
    15541 cccacaacag ccagttttcc acatacttac gcgtcatcag aagcattgcc acgatagata
    15601 acaccatcat gcatgaatcc cagaacggga aagcgtccgg ttgcagttca ggcatcacaa
    15661 cctgtaatcc taatgcttgc atgatcatga ctgccacgcg ggtcaaaaat gcaaacaccg
    15721 gattgataaa taccgtcatc agaccaatcg aaacaacgca aaccgccaac cagctgagcg
    15781 ccttcggcaa tggtaaccag cgaattttca actccgcctc gttctgactg gtttgtcgcg
    15841 accacgcata ccaaccgtaa atattcgcgg caaagaaaaa cacctgtaat agcaggctgg
    15901 catacagctg aatctgaaag aaaataatgc caaacaaggt gacgttgatc aggccaaaga
    15961 agtagttgct gatcttctcc agactggcaa ggccaataca cagcaacccg gcgatcgtgc
    16021 ctaccgcttc gatccatgag agatcataac cgcctgctcc tattggtata tgtaccagga
    16081 tattctgcac actaaaaaaa tccatctttc ccccaaagcg taacgttacg ccttattatt
    16141 cgttatccac gtagtgtagc cgcaaaatcc agcatacgat tgagcggcac cagcgccctc
    16201 tctcgcagcc tttcatcaac atgaacctcg tgattgcttc cttcctgttc taatgcctct
    16261 gcgatggcct gaaggccatt catggccatc cacggacaat gcgcgcagct gcggcaggtt
    16321 gcaccctcac ctgcggttgg tgcttccagt aactctttat ctggcaccgc ctgctgcatt
    16381 ttgtagaaaa taccccgatc ggttgccaca ataagcctct gatgtggcaa tgttttcgca
    16441 gcagcgatca gttgactggt ggaaccgacc gcatccgcca tatcgacaat agcttgtggt
    16501 gattctggat gcaccagtat ggcagcatcc gggtattctt cttgcaagcg ggttaacgcc
    16561 tgagtcttaa attcatcatg cacaatacag gcaccctgcc agcatagaat gtctccaccc
    16621 gtctgttttt gcacgtaacg ccccagatgt ttgtcgggtg cccagatgat tttttcaccc
    16681 aaactatcaa gatgatcaat aagttcgacg gcaatgcttg aagttaccac ccaatctgcg
    16741 cgcgctttta ccgcagcaga agtgttggcg tagacgacga cagtacgatc gggatgggca
    16801 tcgcaaaatg cgttaaattc ttcaacaggg cagccgagat ccagtgaaca ttcagcctga
    16861 agtgtcggca tcagaattgt tttttccgga ctgagaattt tggcggtttc tcccataaat
    16921 ctcaccccag cgactaacaa agtagaagcg ggatgctttg caccgaagcg cgccatttcc
    16981 agagaatcag aaatacagcc accggtttct tctgccagtt gttgaatttc gggatcggta
    17041 tagtagtggg caaccatcac cgcattacgt tcttttagca gacgttttat cttctcgcgg
    17101 taatacgctt tttcatcaat gcttaacggc gtcggcttcg gggggaaagg ataaatcgcc
    17161 gtgtctggat caaacattac gctcatctta ccatctcgtt ttacaggctt aacgttaaaa
    17221 ccgacattag cgtaatattc gctgttatcg gtgtaatttg ttttatatgc taaacaagat
    17281 agtcgaatgg tggggaaaag tcactcgaat tacgcctaat tccaccgacg tagagttgct
    17341 tgcacagtaa gctctgacga tgtcatcagg tttacaaagt gaaattgagg atgcctgaat
    17401 agcaaaaaag cgcctttagg gcgctttttt acattggtgg gtcgtgcagg attcgaacct
    17461 gcgaccaatt gattaaaagt caactgctct accaactgag ctaacgaccc atacgggtgt
    17521 tacctgaaag atttctactc ggggccaatc tttaagattg gtgtgtcgtg caggatgact
    17581 cgcttcgctt gccctacggg ccgttgctgg cgcaacgtta tccttcacgc ttaacatctg
    17641 agtttgatgt taaaattggt gggtcgtgca ggattcgaac ctgcgaccaa ttgattaaaa
    17701 gtcaactgct ctaccaactg agctaacgac ccgagtggtg ggtgatgacg ggatcgaacc
    17761 gccgaccccc tccttgtaag ggaggtgctc tcccagctga gctaatcacc cgataatgcg
    17821 ctggatacta caaactactt ccaccgaacc catcgtaatt agtggtgggt cgtgcaggat
    17881 tcgaacctgc gaccaattga ttaaaagtca actgctctac cgactgagct aacgacccgg
    17941 tggtgggtga tgacgggatc gaaccgccga ccccctcctt gtaagggagg tgctctccca
    18001 gctgagccaa tcacccaata ctacgctgga tactacaact aactatgtta gtggtgggtc
    18061 gtgcaggatt cgaacctgcg accaattgat taaaagtcaa ctgctctacc aactgagcta
    18121 acgacccact ttttcgttgc tttcggtttg tttgatatcc cgtggcaacg gcggcatata
    18181 ttactgattt cagacttgag cgcaacaaaa atttcgatgc agatcactca actgcttatg
    18241 attcgcacga cacgaccaga aataatgcaa cttctggtcg tgtgttatgc attacatcgc
    18301 gttcagacgt ttttgtgcct gtttagcgcc atcagtacca gggtatttac tgataacctg
    18361 ctggtatacg gctttcgctt ttgcggtgtc acctttgtcc tgcatgatga cgccgacttt
    18421 aaacatcgca tcagcagcct ttggtgactt cggatagttt ttcactaccg aagcaaaata
    18481 gtacgccgca tcatcttttt tacccttgtt gtagtttaac tgacccagcc aataattggc
    18541 gtttggcagg taagttgaat cagggtaatt tttgatgaaa ttctgaaatg ccaccattgc
    18601 gtcatcctgg cgggatttat cctgcaccag cgcaatagct gcattgtaat ccgtgtttgc
    18661 atcgccgctt ttcaccggcg cgccagcatt cgcagtacca gcatcagctg tcggcgtcgt
    18721 tgatgccgcc gcaccgcttt gatcgctgct ggttgattgc gccgctgcac caccgctgct
    18781 gaggctgtcg atctgcaaca ggatctgctt ttgccgctcc acgacctgat tcagttgata
    18841 ctgattttcc tgaatctgac cacgcaggga atcaatatcg gattgattat cagaaagttg
    18901 ttgctggagt tgggttaaaa gctggctgtg agcattagaa atacgctcaa gttgagtgac
    18961 gcggtcttcg accgagcctg agccgacact actgattggt gcctgagcaa aagcggccca
    19021 gggggccgct ataccaacca gtaacgacag actcaatagt tgatgtctga agttactgct
    19081 catgcaattc tcttagtaaa ccagtaccgc acgacggttt ttggagtatg ccgcttcgtc
    19141 atgacccagt actgcaggtt tttctttacc gtaagaaacg atggagatct ggtctgcaga
    19201 aacgccttta ccctgcaggt acatcttaac ggcgttcgca cgacgttcac ccagggagat
    19261 gttgtattcc ggagtaccac gttcgtccgc gtgaccttcc acggtgactt tgtaagacgg
    19321 gttgctacgc aggaagtttg catgtgcatc cagcatttga gcgaagtcag aacggatatc
    19381 gtacttgtcc agatcgaagt aaacgatgtt gttctgctgc agctgttgca tttgcagacg
    19441 agcctgctct tcggaagaca tgttgccgtt gccgccgttc gcatccatac cagtgccggc
    19501 acccagcatg ccttcgctgc cgtcattgct ggcgttcttg ttggaagaac atgccgcaat
    19561 tgccataaca ggcagagcaa tcatcagccc tttcagcact ttgttcagtt gcatttcaat
    19621 gattccttta ctattcaatt aattattatc acagatacgg cgaccaggca gggaatttga
    19681 cctgtccatc agttgccgga agacgcgctt tgaaacgccc atctgtagaa accaaattca
    19741 gcacggatcc catcccctga gaagagctgt agattaccat agtgccatta ggtgccagac
    19801 ttggcgtttc atccaggaac gtggacgaca gaacttgtac gcctcccgtt gccagatctt
    19861 gtttggcaat gtgctgctgc ccaccattgg agctgaccat taccataaat ttaccgtcgc
    19921 tgctgacatc cgcatcctgg ttctgcgaac cttcccaggt aatacgttgt ggcgcaccgc
    19981 cgttgatatt cactttataa acctgcggac gaccggcctg gtcagaagta aatgccaggt
    20041 tctggctatc cgggaaccag gtcggttcgg tattgttact gcgaccatca gtcacctggc
    20101 ggatctgacc agaagccaaa tccattacgt acaggttcag actaccggtt ttcgacaagg
    20161 cgaatgccag tttgctgccg tctggcgaga atgcaggtgc accgttgtga cgcgggaatg
    20221 aagccacctg acgtacagcg ccatttgcca gcgtctgaat aaccagcgcg gaacgaccgc
    20281 tttcgaaggt cacataagcc agtttagaac cgtctggtga ccacgccgga gacatcagcg
    20341 gctgcggtga acggtgaacg acaaactggt tgtaaccgtc atagtcagat acgcgcagtt
    20401 catacgggaa ctgaccgccg ttggtctgaa caacgtaggc aatacgggta cggaacgcac
    20461 ctttaatgcc ggtcagtttt tcaaacactt catcactggc ggtatgacca gcataacgca
    20521 gccactgctt gttcactttg tacgagttct gagcaagtac agtacccggt gcgccgccag
    20581 tgtcaacaag ttgataagca acattgtaag aaccatccgg attcggagtg acctgaccga
    20641 caactacagc gtcaatgccc agtgcggacc atgcagatgg ttgtacttcc tgcgcactac
    20701 ccggctgctg tggcagacga gcgcgatcta acggattaaa tttaccgctg ttacgcaagt
    20761 cagcagcaac gatgccgcca atatcttcag gtgccgcacc aggccccgcc cactggaaag
    20821 gaacaacacc aataggacga ccggaatcta caccgctgtc gatcacaatg cggacttcag
    20881 catgcagaac tgatgcccac agtatgagaa aaccaaatgc tactcgtaat gcctgcttca
    20941 tcatatctcc cttatctgga ccgatggccc acgataattt agcagaatgt taacaaacta
    21001 aaatacacaa aactaccaga accccgtggc aaccggtgcc tgatgttgac cgtccgaaca
    21061 gtcaacatcg cgattacggt ttgaagtcca atggcgcgtt tttgaacact tcatatactg
    21121 cctggcttgg tggtttcggg atcttcgcaa gtttagctgc tgccaacgca gcctgacaaa
    21181 gtgcgggatc gccaccttca ggtttgatat ccagtaacat accatcgggt gccagtttta
    21241 tgcgcagcgt acaggttttg cctgcatagg acgatgcgtc atagaactta ctttcgatag
    21301 cagatttaat ctgcccggca tagttattga tatcggcccc tgatgcgcca ttgtttttag
    21361 aattaccact cccggcaggc gaagcattgt tccctttcgc ccctcccccc gttttcggtg
    21421 cattcttacc agagcttagc tcaccgaaaa tatcatctgc ctctgcggcg gctttttctg
    21481 ccgctgcttt cttatcagct gctgcctttt cagcagccgc tttttctgct gcttttttgt
    21541 cggctgcagc tttcttatca gctgcagcct tttcagcagc cgctttcttc tcagcttctg
    21601 ctttggcttt ttcagcagct tcagttgccg ctttctttct tgcttcagct gcagctgctt
    21661 ctgccgcttc cgctttcttc ttcagtgccg cagcggctgc ctcggctttt ttctgcgctg
    21721 cggctttggc ggcttctgct tctgcttttt tctttgcgtc tgcagccgct ttcttcgctg
    21781 cttcttccgc agctttagca tctgcttcgg ccttcgcttt agcatctgcc gccgctttcg
    21841 ctgccgcctc ttcagcttgc ttctgcttta actcggcctg ttttgcggct tcttcagcct
    21901 gctttttctg ctcctgagcc gctaaccgct ctttctcaag ttgcttcagg cgttcctgtt
    21961 cagccgcttg tttctcacgc agttcttcag cagcctgctg ttccttcatc ttgcgctgtt
    22021 catcagaacg cttcgcgctt gattcctggc tttgcatgcg tttgtactgc tcaactaccg
    22081 cacctgaatc aaccatgaca gcgtcgatgg acgaaccacc gccgcctccg gctgaagctt
    22141 ctatattctc atcgaacgaa ctccagatca gcgccgcaaa taagatgaca tgcagcactg
    22201 ctgaaataat tatcgcccgc ttgagcttgt cgttttgttc ggttgccttt gacactctcg
    22261 gtttccaaaa actgttcgcc tgttacccgc tctctttcaa gcaagggaaa cgcagatgtt
    22321 tagataggct gcgtcattaa accaaccgat ttcacacccg cactatgtaa caagttcagt
    22381 gctttaatta tttcatcgta aggcacatct tttgcgccac cgatcagaaa gaccgttttc
    22441 gggttggcct tgaagcggct ggacacttcc gccaccacct gctctggtgg taaacgctcc
    22501 aggcgatctt tctcaaccac cacggtgtac tgaccaatac cagacacttc aacaatcact
    22561 ggcggattat cgttactgct caccgcctgt gattcagtag cgtctggcag atcgacctcc
    22621 acgctctggg tgatgatggg cgctgtcgcc atgaagatca gcaacagcac cagcagtacg
    22681 tccagcaacg gtacaatgtt gatttcggac ttaagttcgc gacgacctcg tccacgcgct
    22741 ctggccatga cttacccctt attgctctcg ctaacggtaa acgcctggcg gtgcagaatc
    22801 gcggtaaact cttccataaa gttgtcgtaa ttcagttcca gtttgtttac gcgctggttg
    22861 aggcggttgt aggccataac tgccgggata gcggcaaaca gaccaattgc agtcgcaatc
    22921 aacgcttctg cgataccggg cgcaaccatt tgcagtgttg cttgttttac cgccccgagg
    22981 gcgataaagg catgcatgat cccccagacc gtaccaaaca gaccaatata cgggctgatg
    23041 gagccaaccg taccgaggaa cggaatgtgc gtttccagat tttcaagttc acggttcatg
    23101 gagatacgca tagcacgcga cgccccttcc actacggctt ccggcgcatg gctattggca
    23161 cgatgcaggc gcacaaactc tttgaacccg ctgtaaaata tttgttccga accagtcaga
    23221 ttatcccgtt tcccctgact ctcttgatag aggcgagaga gttcgattcc agaccagaat
    23281 ttatcttcaa acgcttcggc ttcgcgcgcc gctgcgttaa gaatacgggt ccgctggata
    23341 ataatggccc aggatgcgat tgaaaaacca atcaaaatca acatgataag tttaaccaga
    23401 aggctagcct tcaggaacaa atcaaggata ttcatgtcag tcactgctta aactccgcga
    23461 caatagactt gggaagcgca cgaggcttca ttttgagtgg gtcaacgcaa acaaccagaa
    23521 cctctgcttc attcagcaaa gtattctcgg cgttgacaat acgttgcgtg aaaaccaaag
    23581 aggtgccacg cattgatgtt atttcagtct gtatttcgag catatcgtcg agccgcgcag
    23641 gtgcgtaata ttccaccgtc attttacgta ccacaaaggc aacgcgttca gccatcagcg
    23701 cctgctgact gaagtgatga tgacgcagca tctctgtgcg tgctctttca taaaaagcga
    23761 cgtaactggc gtggtacacc acaccaccgg catcggtatc ttcatagtag acgcgagccg
    23821 gccatcgaaa cagcgttgta ttcactttac atcccggtaa tgcaacaaaa gttagagctt
    23881 ttaaacgctg ctactatacg cgcgcgattg aggtttggga atgagggcaa gttaagggag
    23941 cgtaaaaatt tatgggcctg gagcggccca taatttttta aagagaattc tgatttaaaa
    24001 gaagaagaaa atcagcccga taatgagaac aatatcggca agcaatgggc aaaaaatccc
    24061 ttgccaaaga accttctgcg gacgaaagcc cacgccgtga atcacaccag cacagacggc
    24121 ccacatcagc aataaaccat gccagatttc cagttcactg gtcttcgcgg caaaacgaga
    24181 tgggtcccaa aacatacatc ctgctaacag aagcgccatc acgaaggaaa gcgcccgcag
    24241 ggggcgcttg tccattaccg catacaaggt cgcgataatc ttactcatca gatgtcttct
    24301 tgaccggctt tgcctgattc gacgtgttca agcgccagcg cggtgattac cccaaacgaa
    24361 caggcaagaa gcgttcccag aatccatgcg aaataccaca tttttagctc cttacttagt
    24421 acagagagtg ggtgttacgt tcaatatctt ctttggtgat acgaccgaac attttccagt
    24481 aacaccaggc ggtgtagagc agaatgatcg gtaccagaac caccgcaacc caggtcatga
    24541 cgttaagcgt cagctggctg gaagttgcat cccacattgt cagacttgcg ttcatcatgg
    24601 tgctggacgg catcacaaac gggaacattg cgataccggc tgtcaggatg atgcaggcca
    24661 gcgtcaggga ggagaacaca aacgcccacg cggctttatc catacgtgca gtcaggatgg
    24721 tcagcagcgg cagaaccaca cccagtgccg gaatagccca cagaattggc gtgttgttga
    24781 agttaaccag ccatgcgcca gcttcacgaa ccacttcttt attcagtggg ttagaggctg
    24841 cgtaatggtc cattgtcgat ttcacgacat aaccatcgat accgtacatc acccatacgc
    24901 cagccagtgc gaaacagacc agtgtcacca gcgcagccac ctgagccgtt gcacgggtac
    24961 gcaggtgcag ttcgcccacg gtacgcattt gcagataggt tgcgccctga gtaatgatca
    25021 tccctacgct caccacgcct gccagcaggc cgaacgggtt aagcaactgg aagaagttac
    25081 cggtgtagta cagacgcaga tattcatcaa cgttgaacgg tacgccctgc aacaggttac
    25141 cgaacgcaac accaattacc agcggcggaa cgaagctacc aatgaagatg ccccagtccc
    25201 acatgttacg ccagcgggtt tcttcaatct tggagcggta gtcaaaaccg accggacgga
    25261 agaacaaaga cgccagcacg aggatcatcg ccacatagaa gccggagaac gcagcggcat
    25321 agaccatcgg ccaggcagca aagagtgcgc cgcccgcggt gatcagccaa acctggttac
    25381 cgtcccagtg tggtgcaatg gagttaatca taattcgacg ctcggtgtcg ttacgaccga
    25441 ggaaacgggt gagcatgccc acccccatgt cgaaaccgtc agtgactgca aaaccaatca
    25501 gcagaacgcc aaccagcagc caccagataa aacgcaatac ttcataatcg atcatttgac
    25561 gactcctgtc ttagcgtgcc ggctgagtag tcgtggaaga ctgctcaaag tgatagcgac
    25621 cggttttcag gctgcttggg ccgaggcgtg caaatttgaa cattaagaac aattctgcca
    25681 ccaggaacag ggtatacagg ccgcaaatca gcaccattga gaagatgaga tcgcctgcgg
    25741 tcagtgacga attcgccaca gctgtcggca gcacttcacc gatagcccac ggttggcggc
    25801 catattcagc cacgaaccag cccgcttcta cagcaatcca cggcagcgga ataccgtaca
    25861 gcgcggcgcg cagcagccat tttttctcgc caatgcggtt gcggatgaca ctccagaaag
    25921 agagcgcgat gattgccagc agcaggaagc cacacgccac cataatacgg aacgcgaagt
    25981 acagcggcgc tacacgcgga atggagtctt tagttgcctg ttgaatctgc gcttcagtcg
    26041 catcagccac gtttggcgta tagcgtttca gcagcagacc gtaaccgagg tctttcttca
    26101 tgctattgaa ctggtcacga accgcctggt cggtagaacc agaacgcagt tgttcgagca
    26161 gagagtacgc cttcatcccg ttacgaatgc gttcttcatg ctgcaccatc agctctttca
    26221 ggccgataac cggggtatcc acggaacgcg ttgcaatgat gcccagtgcg taagggatct
    26281 gaatcgcaaa tttgttcgtc tcctcttcct gatcaggaat gccgaacaga gtaaaggcag
    26341 caggcgcagg ttgcgtttcc cactcggctt caatagcagc cagtttggtt ttctgcacgt
    26401 cgcccatttc gtagccggat tcatcaccca gaacaataac agacagaaca gcagccatac
    26461 cgaagctggc agcgatagca aaggagcgtt tagcgaaggc gaagtcacga cctttcagca
    26521 tataccatgc gctgataccg aggatgaaca tcgcgccagt cacataacca gacgctacag
    26581 tgtgaacgaa tttcacctga gcaaccgggt taagcaccag ctcggagaag ctcaccatct
    26641 ccatacgcat agtttcaaag ttgaaatcgg acgcgattgg gttttgcatc cagccgttcg
    26701 caaccagaat ccacagtgcg gacaggtttg aaccgagcgc caccagccag gtgacacaca
    26761 tatgctgaac tttacccaga cgatcccaac cgaagaagaa cagacctaca aaggtggatt
    26821 cgaggaagaa ggccatcaga ccttcgattg ccagcggcgc accgaagata tcccctacat
    26881 agtgggaata gtaagaccag ttagtcccga actggaactc catggtcaga ccggtagcca
    26941 cacccagagc gaagttgata ccaaacaact tgccccagaa cttggtcata tctttataaa
    27001 tctgtttgcc ggagaggacg tagaccgttt ccataatggc cagcaggaac gccataccga
    27061 gcgtcagtgg cacaaaaagg aagtggtaca tcgcggtcaa ggcaaactgt aagcgcgaca
    27121 gttcgactat atctaacatc atgactcctt gctcatcgca tgaagactcc gagagtgaac
    27181 cccgttagaa agggtcacac gcatgcccca atagaaattt gtttgaacac tttcatcata
    27241 ccatgtgaat gcacacaaaa acggcgtaaa gaaaggttgc aaacatgtta ataaaaactc
    27301 aaattgatcc cacgtatata ttacgccgca aaatccttac aataaacagg tttttgttga
    27361 agcaaatttg catttttcga cggtgatcaa tttatagcta aattaccgcc tttcagccaa
    27421 tttgatcgag aacaatttat ctctttttga tgcccatttc caagacttat acattgataa
    27481 atatcaattc ctacaaatta tgttaatcat gtttcaataa ggtaacttta gtgacattta
    27541 tgtttaaaat gtgtgagtta taaggctgaa attagtttcg gttggcaatt aaagataaaa
    27601 agagacgaag caaaaacagc acggttgacc ttaattcttt ttttggtcac tggctaatac
    27661 gacgcagagt ttcaggatta ctaataaagg cataaatcca cccccgccga tattaacatt
    27721 taattaactt ataattatat tataattaac tcacccaggg ttattatttt gttaaaaata
    27781 aagccacata tacgtggctt gagagtgaag ataaacgcaa aattacctga tgcgctacgc
    27841 ttgccagtgt ctacatcatc tctgcaatat attgaatttg cctgcttttg tagaccgaat
    27901 aaagcgttca tgccgtatcc ggcatgaaca aagcgccctt tgtcaacaat ctggccgcgc
    27961 atatgtggct tcggtttgat tgctattcag gcaagccggt aactgaacgt ccgtgactgg
    28021 ccgggtaaaa aaggccccga tagatttgaa ccttgatttt cctcggtggt aatgtgttca
    28081 tccatcatcg tttctgagca agaaatcacc tcgcgactga aagcaacagt cgcatcacag
    28141 gttgctgatt cagccggatt aaacagccgt aaaatcactt cttgtcggtc ttcagctttc
    28201 ttaagtgcgc ttatcaggca tcccactggg ggcattttca acaaactata actttccggc
    28261 acgttgaatc cggctttgtt gagcttcatc gcatcccatg ggattttgtt gtaacactgt
    28321 actggagtca gccatgctcg cgcctgctga gctacaccag cggcggttgg cgtaccggta
    28381 taactcaata aacttaggcg acaagaaagc agaccacgta gttgtgagtc cgggactggc
    28441 attttaattc ccgaaggccg cccaggcctt aaaagcagat cttctttgcc cagtaagccc
    28501 acgccacgca gcaacgtaat ggcaaaggtt ttcttctctt caccgatgac ttcaaattca
    28561 cgtaaccctt cgctaaagac agccatgccg ttacgccctt cctgtaaggc aacatagttg
    28621 agcatattcc ataccggaac cggcgcttct ttccagcctt cttgctgcca gttgttcatt
    28681 gcactgtcgt tcacggggcg cgttagcgaa ccaaactgcg tatctgccag aacactgtcg
    28741 gtgttaaaag gtgtagggac caggacacga aggcgatgat cgtcagcctg gttatcaagg
    28801 ttgatatcca catcaatacg cctgctgtta tgactaagag tgacaaccaa caccacccct
    28861 actctgccag tggattgccg ggcgctgcgt tctgacaaat tgagcggcac tgccatgtca
    28921 tagcggataa cagccctgct ctgccaggct tcatgaataa tatcgcattg cggtttcgcg
    28981 ttcgctgcgg taattaccca ctcttctttt gcgggtgaat agtcatattc atcaccatca
    29041 tcagagcttt cttcaatt
//
LOCUS       DAJVUJ010000033        28862 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.033, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000033 DAJVUJ010000000
VERSION     DAJVUJ010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28862
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.033"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(<1..1684)
                     /gene="fruA"
                     /locus_tag="OQQ48_001376"
     CDS             complement(<1..1684)
                     /gene="fruA"
                     /locus_tag="OQQ48_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HCS1952204.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAVARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAK"
     gene            complement(1701..2639)
                     /gene="fruK"
                     /locus_tag="OQQ48_001377"
     CDS             complement(1701..2639)
                     /gene="fruK"
                     /locus_tag="OQQ48_001377"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCS1952205.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(2639..3769)
                     /gene="fruB"
                     /locus_tag="OQQ48_001378"
     CDS             complement(2639..3769)
                     /gene="fruB"
                     /locus_tag="OQQ48_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HCS1952206.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFAVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            4137..5318
                     /gene="setB"
                     /locus_tag="OQQ48_001379"
     CDS             4137..5318
                     /gene="setB"
                     /locus_tag="OQQ48_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HCS1952207.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALTREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRTKD
                     V"
     gene            complement(5315..5569)
                     /locus_tag="OQQ48_001380"
     CDS             complement(5315..5569)
                     /locus_tag="OQQ48_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HCS1952208.1"
                     /translation="MECRPGCGACCTAPSIFSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAS"
     gene            5724..6296
                     /gene="yeiP"
                     /locus_tag="OQQ48_001381"
     CDS             5724..6296
                     /gene="yeiP"
                     /locus_tag="OQQ48_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HCS1952209.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            6519..7985
                     /locus_tag="OQQ48_001382"
     CDS             6519..7985
                     /locus_tag="OQQ48_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCS1952210.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARTLMLNE
                     QAPTLQIKDVDLTQYADKLIARFANPALKHKTWQIAMNGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            8103..9089
                     /gene="yeiR"
                     /locus_tag="OQQ48_001383"
     CDS             8103..9089
                     /gene="yeiR"
                     /locus_tag="OQQ48_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HCS1952211.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWTVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            9128..9841
                     /gene="lpxT"
                     /locus_tag="OQQ48_001384"
     CDS             9128..9841
                     /gene="lpxT"
                     /locus_tag="OQQ48_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HCS1952212.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGLWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIIGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTNINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            10253..10819
                     /gene="mepS"
                     /locus_tag="OQQ48_001385"
     CDS             10253..10819
                     /gene="mepS"
                     /locus_tag="OQQ48_001385"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HCS1952213.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            11000..12556
                     /locus_tag="OQQ48_001386"
     CDS             11000..12556
                     /locus_tag="OQQ48_001386"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HCS1952214.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGGETPEQARWLSEPGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            12638..14452
                     /locus_tag="OQQ48_001387"
     CDS             12638..14452
                     /locus_tag="OQQ48_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCS1952215.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADTLLNEAGWVLKGQQRVNATTGQQLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSHPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            14453..15547
                     /locus_tag="OQQ48_001388"
     CDS             14453..15547
                     /locus_tag="OQQ48_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HCS1952216.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     HNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTILTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            15547..16572
                     /locus_tag="OQQ48_001389"
     CDS             15547..16572
                     /locus_tag="OQQ48_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1952217.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            16574..18163
                     /gene="yejF"
                     /locus_tag="OQQ48_001390"
     CDS             16574..18163
                     /gene="yejF"
                     /locus_tag="OQQ48_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HCS1952218.1"
                     /translation="MTQTLLAIEDLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFRGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNYAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIIFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKLQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFAAPQQEYTRQLLALS"
     gene            complement(18167..18511)
                     /locus_tag="OQQ48_001391"
     CDS             complement(18167..18511)
                     /locus_tag="OQQ48_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1952219.1"
                     /translation="MTSLQLSIVHRLPQNFRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(18844..20027)
                     /gene="bcr"
                     /locus_tag="OQQ48_001392"
                     /pseudo
     CDS             complement(18844..20027)
                     /gene="bcr"
                     /locus_tag="OQQ48_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
     gene            complement(20055..20750)
                     /gene="rsuA"
                     /locus_tag="OQQ48_001393"
     CDS             complement(20055..20750)
                     /gene="rsuA"
                     /locus_tag="OQQ48_001393"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HCS1952220.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            20899..22659
                     /locus_tag="OQQ48_001394"
     CDS             20899..22659
                     /locus_tag="OQQ48_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCS1952221.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            22784..23068
                     /gene="rplY"
                     /locus_tag="OQQ48_001395"
     CDS             22784..23068
                     /gene="rplY"
                     /locus_tag="OQQ48_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HCS1952222.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLAIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(23207..24214)
                     /gene="yejK"
                     /locus_tag="OQQ48_001396"
     CDS             complement(23207..24214)
                     /gene="yejK"
                     /locus_tag="OQQ48_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HCS1952223.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTKFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            24396..24623
                     /locus_tag="OQQ48_001397"
     CDS             24396..24623
                     /locus_tag="OQQ48_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HCS1952224.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            24643..26403
                     /gene="pbgA"
                     /locus_tag="OQQ48_001398"
     CDS             24643..26403
                     /gene="pbgA"
                     /locus_tag="OQQ48_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HCS1952225.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRPQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALLDSGKLDNTVVIITAG
                     RGIPLSEDEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            26478..26554
                     /locus_tag="OQQ48_001399"
     tRNA            26478..26554
                     /locus_tag="OQQ48_001399"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26512..26514,aa:Pro,seq:ggg)
     gene            26773..28014
                     /locus_tag="OQQ48_001400"
     CDS             26773..28014
                     /locus_tag="OQQ48_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCS1952226.1"
                     /translation="MARKTPPLTTVQIKAARPAEKEYTLQDGGGLFLLVKPSGSKLWR
                     FSYYRPSDKKRILLSFGSLDDVSLADARKRRSEYRALISAGTDPQGHEKKKREAEARR
                     QGNTFENVAAAWYQVKISQNLAPNTIKDIWRSLDKYVFPFIGNTPIDTLTARRFVEVL
                     TPIKERGNLETLKRVLQRVNEVMDYAANSGLIDANPAMNVRKAFPSPVKKHMPTIRPE
                     QLPELMQALSVSATERQTRLLIEWQLLTVTRPAEASSTRWDEINLDAKQWTIPAGRMK
                     MRRDHVIPLSGQAMAVLEAMKPISHHRNYVFPSLKDPQQPMNSQTANAALRRMGFAGV
                     LVSHGLRAIFSTAANEEGFEPDVIEAALAHVDTNEVRRAYNRSNYIEKRIVLMRWWGE
                     FVEAAATGVTLASGKRGIRAV"
     gene            28510..28716
                     /locus_tag="OQQ48_001401"
     CDS             28510..28716
                     /locus_tag="OQQ48_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HCS1952227.1"
                     /translation="MEAIRKIIFRQEVKKIIHIKADSTLQSMINAGEFPQGFRVGLRR
                     RGWYEDDVLAWQKEREQEARGTAA"
BASE COUNT         7028 a         7507 c         7218 g         7109 t
ORIGIN      
        1 ctttcgctac tgcgtccact tccggacgtt tcaggaaggc ataggccaaa cccgccacca
       61 gcgtaccggc aataattgct accaggtaac ccagtaccgg cgtaatagcg ccagggatca
      121 gcagaacaaa cagaccaccg tgcggtgcca tcagtttcgc accaatcgcc attgagattg
      181 cgccagtcag cgccccaccc acgatacagc acggcagcac acgcatcgga tcacgagcag
      241 caaacggaat tgcaccttcc gaaatgaagc acagtcccaa taccagagcg gctttgccac
      301 cttcctgctg cgctttgtcg aatttgcgac gcgccaccat tgttgccaga cccattgcca
      361 gcggtggcac catacctgcc gccataatcg ccgccatcgg gccataggtt tgagtactca
      421 gcagacccac accgaatgcg tacgctgctt tgtttaccgg accgcccatg tcagtacaca
      481 tcatgccacc gaggatcgcc cccagcagaa ccgcattcgc agtccccatg gtctgcagcc
      541 agtgagtcag cccttcgaga atgccagcaa ctggtttacc gatcaggtag atcatcgcca
      601 gaccgaccac cagactggaa attagcggaa tgatcaggat cggtttcagc gcctccatac
      661 tctgtggcag tttcagttgc gtactgatta acttcgcaat gtaaccagcc aggaagccag
      721 caattatacc gccaataaag ccagaaccgg tgctgaccgc cagcataccg ccaatcagac
      781 ccggcgtgag gcccggacga tcggcaatgg agaaggcaat ataacctgcc agtaccggca
      841 ccatcagcgc aaaggctgaa ccaccgccaa tctgcatcag cgccgctgcc aacgtacccg
      901 gctctttaaa cgcttcgata ccaaaagcaa aagaaagcgc gatacacaga ccacctgcga
      961 cgaccatcgg cagcatataa gagacgcctg tcagcaagtg acggtaagcg cctgcactct
     1021 ctttcttacc ttcagtggtc gccgtttgag ctttgcccgc cggttcatac ggcgttgctt
     1081 cagcaaccgc tttatccagt tcctgcgcgg ttttcttcag cgccagaccg gtagaggtac
     1141 gatacatcgg tttaccagca aatttcgcca gatccacttc gatatctgcc gccacaatca
     1201 ccagatccgc tgctgcgact tcttcagggg tgattgcatt gcccgcgcca acagaaccac
     1261 gggtttcaac tttcacccac cagccacgtt ttttcgcttc ggtttcaatg gcttcagccg
     1321 ccataaaggt gtgtgctacg ccagtcgggc aagcagtcac cgcaactacg cgtttcggac
     1381 cgctggcagc aaccggtgct gtcgcagcga ccggcgcagt gtaaggtttc gcatggcctt
     1441 tggcttcact caggaacagc tcagggtgcg caactgcccg ggaaatatca cccagccaga
     1501 catttttacc gttcagcgcg ctgtcattcg ggatggaatc accgagaaca atcgccattt
     1561 ctgcgtcgtt cggattgtcg atgatttcca gttttgcttt tcgcgccacc gcgcccagca
     1621 gggtcttcgc catataggcg cgtgcctgac cgagattagc gtcaataatc agcagcgttt
     1681 tcattatgcc tctcctgctg tcagttaaaa ggttgtaagt cgacgcgcgc catcattgcg
     1741 gccaactgcg gacgatcggt aatacccaca ttgctttgac ttaccgccag ggctgcaaca
     1801 gctgtcgcca gacgcagtgt gtgttcactg gattcacgca tcagcaagcc ataaatcagg
     1861 ccaccaacca tagaatcccc tgcgccaacg gtgcttacga catcgactga cggtggtttg
     1921 gcgatccatt cgccggaggc attaacccaa agcgcgcctt cggcacccag tgaaataaca
     1981 acatgcgcaa taccttgttc acgtagcgca tgtgcagctt caatcacatc tttcatttca
     2041 ggcagtttac ggcctgccca aatttccaac tcgcggcggt taggtttcac cagccacggt
     2101 gccgctttca aacctgctac taacgcttca cggctactat caaagataat gcaaggacac
     2161 tgactacgca ggcgagtcat ccagtcggtg aacgcttccg ggctgacgcc tgacggtaag
     2221 cttccgctga cacagaccat atcgaactga ccgagccagc tcagagaatc agtcacaaag
     2281 cgttcccagt cggcgggggt gacttcaaaa cccgagaagt tgaagtcggt cacttcgccg
     2341 tctttttccg tcagcttaac gttaattcgg gtgcgcccct gtacaacctg gaaacggttg
     2401 gcaatgccca gctcgctgaa cagttgctga aaaccatcct gattgtcttt acccaggaag
     2461 ccgccaacgg tgacatcaat tcccaggtct tttaatactt tggccacgtt gatgccttta
     2521 cccgccgcat gcagaccggt ggttttcacc aggttcactt cgccgcgttc aatttccggg
     2581 cagaaaccaa caaggtcata agccggatta agggtgatcg tagcaacacg tctgctcatt
     2641 atgcgccctc cccaagacca gcagcgatag cgtcgccaat ggctttcagc gcctgttcag
     2701 catctgcacc ctgggcggta aagcgtaggc gatgaccttt cttaacgcca agtgccacaa
     2761 ctttcatcag actacgtccg tttgccggtt tgccggtgcc atcaaggttt gtcacggtaa
     2821 tatcactgtt aaattgttta atggtattga ccagcatggt acctggacga gcatgcaggc
     2881 cgtgttcatt gcgcaccaca aactccgcgc ttaacacgtc gtcggtcggc gcatcatcgc
     2941 tggtcagcag cgccagcaac gttgccgcat ctgctttcag caagcggtca gctttattgt
     3001 cgagcaataa atcagcgaga cgcttaagaa ccgcgatggg ctgatcgtca ttcatcgcca
     3061 cactcaccag catggctgcc gtttcgccgt ccacagcaaa agcatttgcc gcacggctta
     3121 ccgcaatcgc gctacgcaga ttgccttcgg cgctatcgct cagccagata ccctgtccga
     3181 gattcagcgg ttgttcattg atggctttgg tgacgaaagt ggcgtcaact gcccccgcct
     3241 ctttcagacg cgcagcgttc agcgcctgaa gagtcagcag atcgctggcg acgatatcca
     3301 gcgtcagcat ttcgttgtcg agcttcagct gctcactctg cttttcgccc atcagtaatg
     3361 cgcgaagttc ttctgctgtt gttgctgact tcagttgttc agcaacggaa tcatcgctca
     3421 gtacgtgggt cagctggcgt agcaggccca gatgttcatc cgagctggca gcaataccga
     3481 ttgccacgta cgctacctga ccgtcacccc aggtgacgcc ttccgggaac tgaaatacct
     3541 gaacgccggt tttcagcacc tgatcgcggg tgtcggtagt gccgtgtgga atagcaatac
     3601 cattgccgag gaacgttgag gtttgctgtt cgcgcgccag catgccattg acgtagcctt
     3661 ctgctacatt accggcctgc accagcgccg cagcgacctg gcgaatcgcc tcttctttgt
     3721 ctccggcctt ttcgcccgga tggatgtcct gtacggataa ctggaacata gttctcctct
     3781 cttgctgaat tgaaacgatt cagcctctat gagaaaaaaa gcgccaacct ggcttagggt
     3841 taaagacaag atcgcgctga aacgtttcaa gaaagcataa tacttctgtt tcagcacgca
     3901 aggaaagccg taaagttatg agcaaaagtt tgatgtgctg cacatttttt tgtcattttt
     3961 cctaattgct gatgggaaaa tccgttatca gcaatttcat ttcagcagcg tcagcagcca
     4021 atcagcaaca gtgaaatggc attttgatta ttcgtggcgc ttttgatttc gctgtctgtt
     4081 tattttctga caagcagcgt aaactccgcg tcttcctctt ccagtgatag acctgcatgc
     4141 ataactcccc cgcagtctcc agcgcgaaat cgtttgacct gacctcgacg gcgtttttaa
     4201 tcgttgcctt tctcaccggt attgcgggtg ctctgcaaac cccgacactc agtattttcc
     4261 ttaccgatga agtacatgcc cgtccggcga tggtgggatt cttctttacc ggcagcgctg
     4321 tcattgggat tctggtaagt cagtttctcg ctgggcgctc tgataagcgc ggcgatcgca
     4381 aatcgctgat tgtcttttgc tgcctgttag gcgtgctggc ctgcaccctt tttgcctgga
     4441 atcgcaacta ctttgttttg ctattcgttg gcgtctttct tagcagcttt ggctcgaccg
     4501 ctaacccgca aatgtttgcc cttacccgtg aacatgccga caaaaccgga cgtgaggcgg
     4561 tgatgttcag ctctttttta cgcgctcagg tttcactggc atgggtcatt ggcccaccgc
     4621 tggcttatgc gttagcgatg ggtttcagct ttacggtaat gtatctgagc gcagcggtag
     4681 cgtttattgt ttgcggcgtg atggtgtggc tgtttttacc gtcgatgcaa aaagagcttc
     4741 cgctggcgac cggcacggtt gaagcgccgc gccgtaaccg tcgcgatacg ctactgctgt
     4801 ttgtcatttg tacattgatg tggggctcga acagcctgta catcatcaac atgccgctat
     4861 ttattatcaa cgaactgcat cttcccgaga aactggccgg tgtgatgatg gggaccgccg
     4921 ccgggctgga aatcccgacg atgttgattg ccggatattt cgccaaacgt ctgggtaagc
     4981 gtttcttaat gcgcgttgct gccgtgggtg gcgtctgttt ttacgcagga atgctgatgg
     5041 cgcattcacc tgccattctg ttgggcttgc agctgctaaa tgctattttt attggcattc
     5101 tgggcggcat cgggatgctc tattttcagg atttgatgcc cggtcaggca ggttcagcca
     5161 ccacgctcta taccaacacg tcgcgcgtgg gctggatcat cgcaggatca gtggcgggca
     5221 tcgtcgccga gatctggaat tatcacgctg tgttctggtt tgcgatggtg atgattatcg
     5281 ccactctgtt ttgcttactg cggactaaag atgtttaaga cgcggtcagc atttccagat
     5341 cgataagcca ggtcatggcc tgctgacgcg agttaccgca catttcagcg ctggcttgta
     5401 agcctgcaca gactttcggt cgcagtggcg aggtgaaaat tttacagcgt tgctgttcat
     5461 caagctgaat gcagggcgta ttggcgggct tgccatctgg cataccggga atagggctga
     5521 aaattgaagg ggcagtgcaa cacgccccgc aacccggacg gcattccata cgttgttctc
     5581 ttaaggttaa ccaccggatt gatgagcgcg cacagtacca ccttttttgc accagcaaaa
     5641 gtgcgaatac cacttgcccg aaaggcccgt cgcgagtact ttgtcgcgat atttttgaca
     5701 ttttcgacta caggaatttt tcgatgccaa gagcgaacga aattaaaaaa ggtatggtac
     5761 tgaattacaa cggcaaactg ctgctggtta aggatattga tattcagtcg cccactgccc
     5821 gcggtgccgc tacgctgtac aaaatgcgtt tttctgatgt ccgtaccggg ctgaaagtag
     5881 aagagcgttt caaaggtgat gatatcgttg acaccgtgac gctgacccgc cgttacgttg
     5941 acttctccta tgtcgatggc aacgaatatg tctttatgga taaagaagac tataccccgt
     6001 ataccttcac caaagatcag attgaagaag agttgctgtt tatgccagag ggcggcatgc
     6061 cggacatgca ggtgctgacc tgggacggtc aactgctggc gcttgagctg ccgcagaccg
     6121 ttgatctgga aatcgttgaa accgcaccag gcatcaaagg ggcatctgcc agcgcccgta
     6181 acaaaccggc gacattgagc actggtctgg tgattcaggt accagaatac ttaagcccgg
     6241 gcgaaaaaat tcgtatccat atcgaagaac gccgttatat ggggcgtgct gactaacttc
     6301 agccgcatgc agaaaaggga tagctcaggc tgcccctttt ttaatttatt ataccaatct
     6361 tctattttgc gcttcctgaa caccttacgc cctcaatttt cactcgttga ttgatcgccc
     6421 tcacacttca tcgcattaac aatccagacc aatttcaatt gctgtcatat aactttacac
     6481 tgtcattgtt aatttatcgt tactaagacg tgactcctat gaagacaatt gcctccgtta
     6541 cgctcccgca tcatgtacac gctccacgct acgatcgcca gcagttgcaa tcacgtatcg
     6601 ttcattttgg ctttggcgcc tttcaccgcg ctcatcaggc gttactgacc gatcgtgtgc
     6661 tgaatgccca gggcggcgac tgggggatct gtgaaatcag cttgttcagc ggtgatcaac
     6721 tgatgagcca gctccgcgca cagaaccatt tatataccgt gctggagaaa ggtgcggacg
     6781 gcaatcaggt gataattgtc ggtgccgttc acgaatgcct taatgcaaaa ctggattcct
     6841 tagcggcaat tattgagaaa ttttgcgagc cacaggtggc aattgtttcc ctgacgatta
     6901 ccgaaaaagg ctattgtatt gacccggcca ccggtgcact cgacaccagt aatccgcgga
     6961 ttattcacga tctacaaacc cctgaagaac ctcactccgc accgggtatt ctcgtcgaag
     7021 cactgaaacg ccgccgtgag cgcggcctta caccgtttac cgtgctctcc tgcgacaata
     7081 ttcccgacaa tggccatgtg gtgaaaaacg cggtgctggg aatggcagaa aaacgttcgc
     7141 cagaactcgc cgggtggata aaagaacacg tcagttttcc gggaaccatg gtcgaccgca
     7201 ttgttccggc tgcaaccgac gaatcactgg cagaaatcag ccagcatctg ggggtaaatg
     7261 atccctgcgc gattagctgc gaaccgttta tccagtgggt ggtggaagat aacttcgtcg
     7321 ctgggcgtcc tgcctgggaa gtcgcaggtg tacaaatggt gaatgatgtc ctgccatggg
     7381 aagagatgaa actgcgtatg cttaatggca gccactcttt tctcgcttat ctgggttacc
     7441 tttcaggatt cgcccatatc agtgattgca tgcaggatcg cgcatttcgc catgccgcca
     7501 gaacattaat gctgaatgag caagcgccga cactacaaat taaagatgtc gatttaacac
     7561 aatatgcgga caagttaatt gctcgttttg ctaatccggc gctgaaacat aagacctggc
     7621 aaatcgcaat gaatggtagc cagaaattac cgcaacgtat gctggcaggt attcgcatac
     7681 atctggggcg cgaaacggac tggtcgttgc tggcattagg cgttgctggc tggatgcgtt
     7741 acgtcagcgg cgttgatgat gccggaaatg ccattgatgt tcgcgatccg cttagcgata
     7801 aaattcgcga acttgttgcg ggcagcagca gtgaacaacg cgtaaccgcc ctgctttccc
     7861 tgcgtgaagt tttcggtgat gatctgccag ataacccgca ttttgtgcag gccatcgaac
     7921 aagcctggca acaaatcgta caattcggcg cacatcaggc gctattaaac accctcaaaa
     7981 tttaacgatt tctgcggtta aagcggatga agctcacctt cgtccgctct ccccttctct
     8041 tttctgcctt ttttagccag gattaacgct cagttaactt accagagtat tgcggagccg
     8101 ttgtgaccag gaccaacctc atcaccggtt ttctcggcag cgggaaaacc acgtcgattc
     8161 ttcatctgtt agcccataaa gatcccaacg aaaaatggac ggtactggtt aatgaatttg
     8221 gggaagtcgg aattgatggt gctttgctcg ccgatagcgg cgcattgctg aaagagatcc
     8281 ccggcggctg catgtgctgc gttaatggtt tacccatgca ggtagggttg aataccttac
     8341 tgcgtcaggg aaaaccagac cgcttgttga tagagccgac cgggctgggc catccgaaac
     8401 agatcctcga tctgttaacc gcaccagtct atgaaccgtg gatagatctg cgcgccacct
     8461 tgtgcattct cgatccacgc ttgctgctgg acgaaaaaag cgccagcaat gaaaacttcc
     8521 gtgaccagct ggctgccgca gacatcattg tcgccaataa atccgaccgt gcgacgcccg
     8581 aaagtgagca agcgctacag cgttggtggc agcaaaatgg tggcgatcga caattaattc
     8641 acagtgagca tgggaaagtt gacggtcatc ttctggattt gccgcgtcgc aatttagccg
     8701 agttgcccgc cagcgccgcg cattctcatc agcatgtcgt gaaaaaaggg ttagcagcgt
     8761 taagcctgcc agagcatcaa cgctggcgtc gcagtctgaa cagcgggcaa ggatatcagg
     8821 cctgcggctg gatattcgac gctgatacgg tattcgacac cattggcatt ctggaatggg
     8881 cgcgacttgc accggtggaa cgcgtcaaag gcgtgctgcg tattcccgaa gggctggtac
     8941 gaatcaaccg tcagggcgat gacctgcaca ttgaaacgca aaacgttgcg ccaccggaca
     9001 gccgtattga gctgatttcc agcagcgaag ctgactggaa tgccttgcag agcgcgctgt
     9061 tgaagcttcg tttagcgact accgcgtaag gttgcctgcg tttttcagta agataattag
     9121 agaaaatatg attaaaaatt tgccgcaaat agtgttgttg aatattgtcg gcctcgcgct
     9181 gtttctttcc tggtatattc ccgttaatca tggattatgg ttgccgattg atgcggatat
     9241 tttttatttc tttaatcaga aactggtcga aagtaaggcc tttttatggc tggttgcatt
     9301 gaccaacaat cgcgctttcg acggttgttc tctgctggcg atgggaatgt tgatgctgag
     9361 tttctggctg aaagaaaacg ccccaggcag acgacgtatc gtgattattg gtctggtaat
     9421 gctattaact gcagtggtat taaaccagct gggtcaggca ttaattcctg taaaacgggc
     9481 cagcccaaca ttgacgttta ctaatattaa ccgcgtcagc gaactgctct ctgtccccac
     9541 gaaagatgcc tcacgagata gctttcccgg cgatcacggc atgatgctgc ttattttttc
     9601 ggcattcatg tggcgttatt tcggtaaagt tgcaggtctt atcgccctta ttatttttgt
     9661 ggtttttgcg tttcccagag taatgattgg cgcacactgg tttactgaca tcattgtcgg
     9721 ttcgatgacc gtgatattga tcggtttgcc ctgggtgttg ctgacgccat taagtgatcg
     9781 attaatcacc ttttttgaca aatcactacc gggaaaaaac aaacatttcc aaaacaaata
     9841 actcacagta attaacatca tcaggtttat ttttatagtg aggataatcc tgatgatgcg
     9901 caccgtgctt tcatctatcg aacgcaaaaa tcattctcta agtaaatgaa tggattgcat
     9961 gcgtttcact caatcgtact ttaattgacc aatcccgctt attaactttc tgtatcactt
    10021 tttcttataa aaaatcatgt aaaaccgctc gccaagaccg caccaatcgg gtaatctcga
    10081 actcgttttg cctcggcggt agattatcct cacagcatat aattttgtgc gttagtccac
    10141 agatttggcc ttaaggaatt gtttcaacat gcccaggtaa ttagtctcgt gtcgcttggc
    10201 atttttttat aacgatattt gtcgttaagg acttcaaggg aaaacaaaca acatggtcaa
    10261 atctcaaccg attttgagat atatcttgcg cgggattccc gcgattgcag tagcggttct
    10321 gctttctgca tgtagtgcaa ataacaccgc aaagaatatg catcctgaga cacgtgcagt
    10381 gggtagtgaa acatcatcac tgcaagcttc tcaggatgaa tttgaaaacc tggttcgtaa
    10441 tgtcgacgta aaatcgcgaa ttatggatca gtatgctgac tggaaaggcg tacgttatcg
    10501 tctgggcggc agcactaaaa aaggtatcga ttgttctggt ttcgtacagc gtacattccg
    10561 tgagcaattt ggcttagaac ttccgcgttc gacttacgaa cagcaggaaa tgggtaaatc
    10621 tgtttcccgc agtaatttgc gtacgggtga tttagttctg ttccgtgccg gttcaacggg
    10681 acgccatgtc ggtatttata tcggcaacaa tcagtttgtc catgcttcca ccagcagtgg
    10741 tgttattatt tccagcatga atgaaccgta ctggaagaag cgttacaacg aagcacgccg
    10801 agttctcagc cgcagctaat aaaccgtttg gatgcaatcc cttggctatc ctgacgagtt
    10861 aactgaaagc actgcttagg tagtgctttt ttgttttcat tcatcagaga aaatgatgtt
    10921 tccgcgtctt gatccaggct atagtccggt cattgttatc ttttaaatgt tgtcgtaatt
    10981 tcaggaaatt aacggaatca tgttcatacg cgctcccaat tctggacgta agctcctgct
    11041 tacctgcatt gtagcaggcg tgatgattgc gatactggtt agctgccttc agtttttagt
    11101 ggcctggcat aagcacgaag tcaaatacga cacactgatt atcgacgtac aaaagtatct
    11161 cgatacctat tttgccgacc tgaaatccac tactgaccgg cttcagccgc tgaccttaga
    11221 tacctgccag caggctaacc ccgaactgac cgctcgcgcg gcgtttagca tgaatgtccg
    11281 tacgtttgtg ctggtgaaag ataaaaaaac attctgttca tctgcgactg gtgagatgga
    11341 cattccactc aatgaattga ttccggcgct cgacattaat aaaaacgtcg atatggcgat
    11401 cttacccggc acgccgatgg tgccgaacaa acccgcaatc gtcatctggt atcgcaaccc
    11461 tttgctgaaa aatagcggtg tgtttgccgc tctgaatctc aacctgacgc cttctctctt
    11521 ttatagttca cggcaggaag attacgatgg cgtcgccctc attattggca atactgcgct
    11581 atctaccttt tcttcacgtt tgatgaacgt taatgaatta accgacatgc ctgtccgtga
    11641 aactaaaatt gcgggcattc ctctgaccgt tcggctttat gcggatgact ggacatggaa
    11701 cgatgtgtgg tacgcatttt tactgggcgg catgagtgga actgtcgttg gcctgctctg
    11761 ctattacctg atgagcgtac gtatgcgccc cggcagagaa atcatgaccg ccatcaagcg
    11821 cgaacaattt tacgtggcgt atcaaccggt ggtggataca caagctttgc gagtaacggg
    11881 cctggaagta ctgctacgct ggcggcatcc tgtcgcggga gaaattcccc cggatgcctt
    11941 cattaacttt gccgaatcgc aaaagatgat tgtgccgctg actcagcacc tgtttgagtt
    12001 aattgcccgc gatgccgcag aattagaaaa agtgctgcca gtaggcgtca aatttggtat
    12061 taacattgcg ccggaccatc tgcacagcga aagctttaaa gcagatatcc agaaactgct
    12121 cacttccctg cccgcacacc atttccagat tgtgctggaa attaccgagc gcgatatgct
    12181 gaaagagcaa gaagccacac aactcttcgc ctggctgcac tcggtcggcg tagaaattgc
    12241 tattgatgac ttcggcaccg ggcacagcgc gcttatctat cttgagcgtt ttacgctcga
    12301 ttatctgaaa attgaccgtg gatttatcaa cgccatcggt acggaaacga tcacttcacc
    12361 cgtacttgac gcggtgctga cgctggcgaa acgcctcaat atgctgacgg ttgctgaagg
    12421 gggcgaaacg ccggaacagg cgcgatggct aagcgaaccc ggcgttaatt tcatgcaagg
    12481 ctactggatt agtcgcccgt taccgctgga cgattttgtt cgctggctga agaaaccgta
    12541 tacgccgcag tggtaaggtg tgcttacgtc ccttattatt catagtgaaa gcatgccgga
    12601 ttgcggctaa tgatgagtaa aaggaaatcc gttgcagatg attgtgcgca tactgctgct
    12661 gtttatcgcc ctgttcacct tcggtgcgca ggcgcaggct atcaaggaaa gctatgcctt
    12721 tgccgtgctg ggcgaacccc ggtacgcatt taatttcaac cattttgatt atgtgaaccc
    12781 cgccgcgcca aaaggtgggc agataacgtt gtcagccctc ggtaccttcg ataatttcaa
    12841 ccgctatgca ctgcgcggca acccgggcgc acgcaccgag cagttgtacg acacgctatt
    12901 tacgacttcc gatgacgaac caggcagtta ttacccgctg attgctgaaa gtgcacgcta
    12961 tgctgacgat tattcctggg tggaggtcgc tattaatccg cgcgcccgtt ttcatgatgg
    13021 ttcgcccatt actgcccgcg atgtagagtt tacttttcaa aaatttatga ccgaaggcgt
    13081 gccgcaattt cgtctggtct acaaaggcac caccgtcaaa gccattgcac cgttaaccgt
    13141 gcgcattgag ttagctaaac ccggcaaaga agatatgctg agtctgtttt cgctgccggt
    13201 atttccagaa aagtactgga aggatcacaa acttagcgac ccgctcgcca cgcctccgct
    13261 tgccagtggt ccgtaccgcg ttacgtcctg gaaaatgggg caaaatattg tctattcccg
    13321 tgtgaaagat tactgggcag caaacttacc ggtaaaccgt ggacgctgga atttcgacac
    13381 cattcgctac gattattacc tcgatgataa tgtcgccttt gaagcgttta aagcaggtgc
    13441 ctttgatttg cgtatggaaa acgacgctaa aaactgggcc acgcgttata ccggtaaaaa
    13501 tttcgataaa aaatacatca tcaaagatga gcaaaagaac gaatcagccc aggatacgcg
    13561 ctggctggcg tttaatatcc aacgtccggt attcagcgat cgccgggtcc gggaagcaat
    13621 cactctcgcc tttgactttg aatggatgaa caaggcgttg ttttacaacg cctggagtcg
    13681 cacgaacagt tattttcaga ataccgaata cgcggccaga aattaccccg acgccgcgga
    13741 gctggtgctt ctggcaccaa tgaaaaaaga tctaccgcca gaagtcttca cacaaatcta
    13801 ccagccgccg gtatctaaag gcgatggcta cgatcgtgac aacctgttaa aagccgacac
    13861 acttctcaac gaagcgggct gggtgctgaa gggtcagcaa cgcgttaatg ccacaacggg
    13921 tcagcaactc agctttgaat tattgcttcc cgcaagcagc aatagtcagt gggtattgcc
    13981 gttccagcac agcctgcaac ggctgggtat caacatggac attcgcaagg tggataactc
    14041 gcaaatcacc aaccgcatgc gcagtcgcga ctatgacatg atgccgcgcc tatggcgggc
    14101 gatgccgtgg cccagttccg atttacagat ttcctggtca tcggaatata tcaattccac
    14161 ttataatgcc cccggcgtgc aaagcccggt tatcgactca ctgatcaacc aaattattgc
    14221 cgcgcaggga aataaagaaa aattattgcc gttggggcga gcactggatc gcgtattaac
    14281 gtggaattat tacatgctgc caatgtggta catggcggaa gaccgtctcg cctggtggga
    14341 taaattctcc caccccgctg tacgccctgt ttacagcctg ggtatcgata cctggtggta
    14401 tgacgttaat aaagcggcca aactgccgtc agccaggcaa cagggagagt agatgggcgc
    14461 ttacctgatt cgccgtctgt tgctggtgat cccaacatta tgggcgatta tcaccatcaa
    14521 ctttttcatc gtgcaaattg cgcctggcgg tccggtcgac caggccatcg ccgccattga
    14581 gtttggtaat gccggagtat tacccggcgc aggcggtgaa ggtgttcgtg ccagccatgc
    14641 gcaaacgggt gtcggcaata tcagcgacag taattaccgt ggcggacgcg gattagatcc
    14701 agaagtgatc gctgagatca ctcatcgcta cggttttgat aagccgatcc acgaacgtta
    14761 cttcaaaatg ctctgggact acatccgctt tgattttggt gatagcctgt ttcgcagcgc
    14821 ctcggtgctg acgctgatta aagacagtct gccggtttcc atcaccctcg gattgtggag
    14881 cacgctgatt atctatctgg tgtcgattcc gttaggcatt cgcaaagctg ttcataatgg
    14941 gagccgcttt gacgtctgga gtagcgcatt tatcattatc ggctacgcca ttccggcctt
    15001 tttgtttgcc atcctgctga ttgtcttctt cgcgggcggc agctatttcg acctgttccc
    15061 tctacgcggc ctggtttccg ctaactttga ttcgctgccg tggtatcaga aaatcaccga
    15121 ttatctgtgg catatcacgc tgccggtgct ggcgacggtg attggtggct ttgcggcgct
    15181 gaccatactg acaaaaaact cattccttga tgaagtgcgc aagcaatacg tggtgaccgc
    15241 gcgtgcgaaa ggggtaagtg aaaaaaatat tctctggaaa catgtgttcc gcaacgccat
    15301 gctgctggtg attgccggtt ttccggcgac gtttatcagc atgtttttta ccggctcgct
    15361 gctgattgag gtgatgtttt cactcaatgg tctgggctta ctgggctacg aagcgaccgt
    15421 ctcgcgcgat tatcctgtaa tgtttggtac cttgtatatt ttcaccctga ttggcctgct
    15481 gctgaatatt gtcagtgata tcagctatac gctggttgat ccgcgtattg attttgaggg
    15541 acgttaatgt cgcgactcag ccccgtcaat caggcccgtt gggcgcgttt tcgtcataac
    15601 cgtcgcggct actggtcgtt atggattttc ctcgtcttgt ttggtttgag tttgtgttca
    15661 gagcttatcg ccaacgataa accattgctg gtacgttatg acggcagttg gtatttcccg
    15721 ttgttgaaaa actacagcga aagcgatttt ggcggcccgc tggcaagtca ggctgattat
    15781 caggacccgt ggctgaaaca acggctggaa aataacggct gggtactgtg ggcaccgatt
    15841 cgctttggtg ctaccagtat caactttgct accgataagc ccttcccttc tccaccctcc
    15901 cggcaaaact ggctgggaac ggatgccaac ggcggcgatg tgctggcacg cattctctat
    15961 ggcacgcgga tctcggttct gtttggcctg atgctgactc tctgttccag cgtgatgggc
    16021 gtgctggcgg gggcgctaca aggctattac ggcggtaaag tcgatctctg gggacaacgc
    16081 tttattgaag tatggtcggg gatgccgacg ctgtttttga ttattttact ttccagcgtc
    16141 gtacagccta acttctggtg gctgctggca attactgtct tgtttggctg gatgagtctg
    16201 gtcggcgtgg tgcgggcgga gtttttacgt actcgtaatt tcgactacat tcgtgcggcg
    16261 caggcgcttg gcgtcagcga tcgcagtatc atcctgcgtc atatgttgcc taatgccatg
    16321 gtcgcgaccc tcaccttttt accgtttatt ttatgtagtt cgatcaccac cctgacctca
    16381 ctcgatttcc tcggcttcgg tctgccgctc ggttcaccgt cactcggtga actgctgtta
    16441 caagggaaaa ataaccttca ggctccgtgg cttgggatca ccgccttctt gtcggtggcg
    16501 atattattgt ctttgctgat ctttattggt gaagccgtcc gcgacgcatt tgatcctaat
    16561 aaggcggtgt agcatgacgc aaactctgtt agcgattgaa gatttgtcgg tgggttttcg
    16621 ccatcagcaa accgtacgta cagtagtcaa tgatgtttca ctacagattg aggctggcga
    16681 aacgctggcg ctggtgggtg agtcaggttc aggcaaaagc gttaccgcgc tgtcaatttt
    16741 acgcctgctc ccttccccgc cggttgaata tctctccggc gatattcgtt ttcgtggcga
    16801 atcgctgctt catgccagcg atcaaacgtt gcgcggtgta cgcggtaata agatcgccat
    16861 gatttttcaa gagccgatgg tgtcgttaaa tccattgcat accctggaaa aacagcttta
    16921 tgaagtgctt tcactccacc gcgggatgcg tcgggaagcg gctcgaggcg aaattcttaa
    16981 ctgccttgat cgcgttggta tccgccaggc ggcaaaacgg ctgacagatt atccgcatca
    17041 gctctccggc ggcgaacgcc agcgggtgat gattgcgatg gcgctgttaa cgcgaccgga
    17101 attattaatt gccgatgaac cgaccaccgc actggacgtc tctgtccagg cgcagatttt
    17161 acagctgttg cgcgaactgc aaggcgagct gaatatgggc atgctgttta ttactcataa
    17221 cctcagcatt gtcagaaaac tggcccaccg cgtggcggta atgcaaaacg gtcgctgtgt
    17281 cgagcaaaat tacgccgcta cgctatttgc ctcacccact catccttaca cacaaaagct
    17341 actcaacagt gaaccgtcag gcgatccagt gccgttgcca gaacctgcct caacgttgct
    17401 ggatgttgaa cagcttcagg ttgccttccc cattcgcaaa gggattttga agcgcattgt
    17461 ggatcataat gtggtggtga aaaacatcag ttttacgcta cgagcgggtg aaacactggg
    17521 tttagtgggc gagtccggtt ccgggaaaag tacgacggga ctggcgctgc tgcgactgat
    17581 taattctcag ggcagcatca tctttgacgg tcagccactg caaaatttaa atcgccgcca
    17641 gctgttacct attcgtcatc gcattcaggt ggtatttcag gatccaaact cctcgctcaa
    17701 cccacgactc aacgttttgc agattattga ggaaggctta cgggttcacc agccgacgct
    17761 ttctgccgca caacgcgaac aacaagtgat agccgtgatg catgaagtgg gattagatcc
    17821 tgaaacacgc caccgttatc cggcggagtt ctctggtggt cagcgacaac gtattgcaat
    17881 tgccagggca ttaattctta agccctcgct gatcatactt gatgaaccga catcatcact
    17941 cgacaaaacg gtacaggcgc aaatattgac gctattgaaa tcattgcaac aaaagctgca
    18001 actggcctat ttgtttatca gccacgattt gcacgttgtc cgcgcgttat gtcatcaggt
    18061 tatcgtactg cgacaagggg aagtagtgga acaaggaccg tgcgcgcgcg tgtttgccgc
    18121 accgcagcag gagtatacgc gtcagctact ggcgttgagc tgacgcttaa aaaggattgt
    18181 agtttgaaaa gggttcggca attgccacac caaaattttt caatcggcat gttgcagcaa
    18241 actcgtcctg gcgatttaca aacaggcacg gctcaccttc acactccagc accgaacacg
    18301 gaacttcgat gtcgcttaac gcctggctta gtttgtacat caccgaccat gcattatcac
    18361 catccgggct aagcaattta agcgccacca ggctgttgtc accgcacggt ccattttgcg
    18421 gaatcggttc aaccttcgaa cctgcgaaac cagcagacca gcgaaagttc tgcggtagac
    18481 gatggacgat tgagagttgt aatgaagtca cttattttcc ccggaagcac atttacttca
    18541 caatttgttt acatcaattt taacacatca tcaacaatcc gtctttatac agatcgtaaa
    18601 gattgatgcc tcgcctgcgt ggcctctatg gctctgattt aagtaatatc tgcgggcgtg
    18661 gtcacgtttg cggggctata tgtacccgtt tctgcgatct aactcaacct ttttaactac
    18721 aatgatgtga ctttttacat aaattgattt tacataaaat aaacatatat cgggggaatg
    18781 atagatttgt ggttgacatg catcaacaaa ggaagccttt tagcttcctc gttgtgcaat
    18841 agatcaccgt tttttcggtc gactggcgta cagacagaag agaatggagc tgcttgcgca
    18901 gaatgcaatt gaccaaatca tcggccatgc agagttaaag gtcgcaagag aaagcaatgc
    18961 gccaacaatt gcccctatgc caaaacggaa ggttcctgcc agcgaagatg ccgttcccgc
    19021 catatgggga aactcatcaa gaatgaccgc catcgcattg gatgacacca tcgacacaca
    19081 gcccacaaac gccgcaacgc caaccaccag aatcccagcc ccagcagcgc actgatgacc
    19141 atccacgctg ccataataaa ttgtatccac aaccccgagc ggaacatatt taacgcgcca
    19201 atgcggcgaa cgaagcggct gttaaagatg gtcatcacga acaggaaaac aatgtttagt
    19261 gcaaagtaat aaccaaagtt ttccggcgcg acgtggttaa tttcaatgta gacaaacggt
    19321 ccggcgctca ggaacgagaa cattccggca aagctgaaac cgctggccag catataactc
    19381 aggacacgct tatgacggaa cagcgccgca aagttaccaa tagtagtacg aatgtgaaaa
    19441 ggctggcgac gctccggtgg taaggtttct ttgatcagaa agaagatcat tgccgaagcc
    19501 agaatcgccg ccacggcgag gatccagaag atgtaatgcc agctcagcca caccagcacc
    19561 cagccgccaa ctatcggtgc catcagcggt gcaatggtcg tcaccagcat gacaaacgac
    19621 atcatccgcg agaactcttc tttcgggtag atatcacgca tcaaagcgtt gataaccatg
    19681 ctggccgcag ccgcagccag cccgtggaaa aaacgcatca caatcagctg atcgatggtt
    19741 tgtgccaacg cacacgccac cgcggcggcg gcaaacacca gcgtaccgcc gagcaccacc
    19801 ggcttacgcc cgaagctgtc tgccatcggc ccgtagatta actgccccaa cgcaaagccc
    19861 agaatataag tactgagggt catctgcgta ctgcccgccg gtacgccaaa ctgcgctgaa
    19921 attaccggta gcgcgggcag atacatatca atcgacagcg gcatcaacat ggccagcagg
    19981 ccaaggataa aaacaatagc aaacgacgaa tgctgtcggg tggtcacaac gggctcctga
    20041 aagtcattga aaagttagac gacgctggca atttcttctt cagttaacgg acgatattca
    20101 ccgggggcta aatcagcatc cagcgtaata ccgccaatac gttcacgatg cagctcaacc
    20161 acgtggttac ccacggcggc gaacatgcgt ttcacctgat gataacgccc ttcgctgatg
    20221 gtcagacgaa cctgcgttgg ggtaatcact tccagcaccg caggcttagt gagatctttt
    20281 tcgttatgca gctgcacgcc tttagcaaat tgctctgccg tatcgtcagc tacaggtgat
    20341 tccagtgtca ccagataggt cttctcgcaa tgatggcgcg gagaagtaat gcggtgcgac
    20401 cactgaccat catcagtcat cagcaccaga ccggtggtat cgatatccag acgtcctgcg
    20461 gcgtgcagtt tccaggctac cggttcatcc agaaaataga gcaccgttgg gtgatcaggg
    20521 tcgtccgtgg agcaaacata gccctgaggc ttattgagca tgaagtaacg tggaccatgt
    20581 tgctgcgcca gcgggttgcc atcgtaagcg acatcatgtt caggaagcag tttgaacgct
    20641 gcattacgaa caatttcgcc atcgacggtg acacgattgc cgcggatttc acgcccggca
    20701 atagcacggc taacgccgag ttgctgtgcg ataaatttat caagtcgcat gtgtgtgatt
    20761 ttgcctgtaa aaacggaggt cgggcattga cccgaaaatc tgaactgttg tctgcccagt
    20821 atagcggtct aataacatcc ctcaagggaa aaagatccat ggcatactat tagcagaata
    20881 atctacctac gcgagaccat gatttttaca ctccgcccat atcagcaaga agccgtggat
    20941 gccacgctca accattttcg tcgtcataaa acccctgccg ttatcgtgct gcccaccggc
    21001 gcaggtaaaa gcctggtgat tgccgaactg gcacggctgg cgcgtggtcg tgttctggtg
    21061 ctggcacacg tcaaagaact ggtggcgcaa aaccatgcaa agtatcaggc gctggggctg
    21121 gaagccgata tttttgccgc tgggttaaag cgcaaagaga gccacggtaa agtggtattt
    21181 ggcagcgtgc agtcggtcgc tcgtaatctt gatgcctttc agggtgaatt ttcgctgttg
    21241 attgtcgatg aatgccaccg tattggtgac gatgaagaga gccagtatca gcaaatcctc
    21301 actcacctga ctaaagtgaa tccccactta cgcctgctgg ggctgactgc cacgcctttt
    21361 cgattgggca aaggctggat ctaccagttc cattatcacg gcatggtacg cggcgatgag
    21421 aaagcccttt tccgtgactg catttatgag ctgccgctgc gttatatgat taaacacggc
    21481 tatctgacgc cgccagaacg actggatatg ccagtagtgc aatacgattt cagccgcttg
    21541 caggcacaga gtaacgggct gttcagcgaa gctgatctca accgtgagct gaaaaaacag
    21601 caacgcatta cgccacatat catcagccag atcatggagt tcgccgaaaa gcgcaaaggg
    21661 gtaatgattt tcgccgccac cgttgaacac gcaaaagaga ttgtgggatt actgcctgcc
    21721 gaagatgcag cactgattac tggcgacacc cccggcgctg agcgcgatgt gttaattgaa
    21781 gattttaaag cccagcgttt tcgctatctg gtcaacgtcg cggtactgac caccggattt
    21841 gacgccccgc acgtcgatct tatcgccatt ctgcgtccta ccgaatcagt gagtctttac
    21901 caacaaattg tcgggcgcgg tctgcgtctc gctcctggca agactgattg cttaattctt
    21961 gattatgcgg gtaatcctca cgacctctac gcgccggaag ttggtacacc gaaaggcaaa
    22021 agtgacaacg tcccggtaca ggttttctgc cctgcctgcg gttttgccaa caccttttgg
    22081 gggaaaacga ccgccgacgg gacattgatt gaacactttg gtcgtcgctg tcagggatgg
    22141 tttgaagatg acgacggcca tcgcgaacag tgtgacttcc gtttccgttt taaaaattgc
    22201 ccgcaatgta acgcggaaaa cgatattgcc gcccgccgct gccgcgaatg tgacacggta
    22261 ctggttgatc cggatgatat gttaaaagcg gcgctacgac tgaaagacgc gctggtatta
    22321 cgctgtagcg gcatgtcttt acagcatggg cacgacgaga aaggcgaatg gttgaaaatc
    22381 acctattacg atgaagacgg cgcggatgtg agtgagcgtt tccgtctgca aacgcccgcc
    22441 cagcgaactg ccttcgagca gctttttatc cgcccgcata cgcgcacacc gggcatccct
    22501 ctgcgctgga tcaccgccgc cgatatcctc gcccagcaag ccttactgcg acatccggat
    22561 tttgtcgtcg cccgcatgaa aggtcagtac tggcaggtgc gtgaaaaagt gttcgattac
    22621 gaaggtcgtt ttcgtcgggc gcacgaatta cgcggttaat ggtagttttg attgatgtat
    22681 aaggcgattg agtatagaat ctcgcccgct tttgcatacg caaagcagat cacttacctg
    22741 ttgctgggtc gcctgtagca ggattaattt aagagagaaa gaaatgttta ctatcaacgc
    22801 agaagtacgt aaagagcagg gtaagggtgc gagccgccgc ctgcgtgccg ctaacaagtt
    22861 cccggcaatc atctacggtg gcaaagaagc accgctggct atcgagctgg atcacgacaa
    22921 agtcatgaac atgcaagcta aagctgaatt ctacagcgaa gttctggcca tcgttgttga
    22981 cggtaaagaa atcaaagtta aagctcagga cgtacagcgt cacccgtaca aaccgaagct
    23041 gcagcacatc gacttcgttc gcgcttaatt gctgaataag ttgtaaaaaa ccccgctccg
    23101 gcggggtttt ttgtatctgc agattatgcc tgattacggt attgctattt tttgcaggcc
    23161 agataaggcg ttcacgccgc atctggcatt cagaatacaa aaccgattaa ttgccgccag
    23221 acgtccggcg ctgcaattgg tcgcgcaaat tcggcggcgt gcctttaatg gtcaaggtat
    23281 cggttgctgg atcccagaag atgcgttcac ctaacagcat cgcatcaaag ttaatagtca
    23341 agccaccgcc gctaccggca aacttcgtca actgacgcag tgtgctacgg tctgccggga
    23401 agctctcttc cagttcgtag cctttttcag ccgcaaactt cgtgaagctc acttcgctta
    23461 cgcctgccag ctctttcgac agcgattcca gttcgatctc ttcgcctgcc tgcagctgct
    23521 cattacagta gctataaacc tgctggcgca cgttctgtcg ctctgcttta tccagttgtg
    23581 cttcggcagt gaaatcatca accgcttgca acagcccacg gttttgcgct ttcgcgttca
    23641 atccttcgct ggccccgagg aaatccataa agaaatccgc cactttgcgc cctactcgcc
    23701 ctttcaggaa agtgagatag cgggtggact ctggattggt ttcccattcg gttaaatcga
    23761 tacgcgcaac aatatccgca tgattgatat caagataatg ggttgggttg atgtcgagat
    23821 tttcgttaac gcgcatactg ctcaggttgc tcagaactgc caccagcaga tactccaccg
    23881 ccagataacg gtagtggcaa aacagcacga atccaccgtc agcaaaagga tatttcgcta
    23941 attcgtcacg caggcgcccg gttgccgcgc ggctaaatgc cagaaagtcc tcttcgccct
    24001 gacgctgtaa gcgcagcgtc tgcgccagtt cactctcttc gctaaacaag ccgtaagctt
    24061 tatttttggc gctatagacc cgatgcagtt ctgccaccat ctcaacgacg gtttctgtcg
    24121 gttccagcaa tgaatcgcgc aacaccagct caaggttctg ctcatcacgc ttgataagct
    24181 ggtgcagagc aatctggttg atatccagac tcatgataaa ctctccttta agaccgggcg
    24241 gtattcaacc accgccggtt aagcgacgca ataaaatatg gcggtcattt tacagaaggc
    24301 agcgtaacga gaatgattta acgcataaaa aagcagaaaa aaaaccgttg ctacggtaat
    24361 atgttgccct ttcatcaact aactgatttc gatttatgcc acaaatttcc cgctacagtg
    24421 atgaacaggt tgaacaattg ctcgcagagc tgctcaacgt actggaaaaa cataaggctc
    24481 cgaccgacct ttccctgatg gtgttaggca acatggtcac taaccttatc aacaccagca
    24541 ttgccccggc ccaacgccag gcaattgcca actcttttgc ccgcgcctta cagtcctcta
    24601 tcaacgaaga caaagcgcac taagggaaac agataacagg ttatggtaac tcatcgtcag
    24661 cgctaccgtg aaaaagtctc ccagatggtc agttgggggc actggtttgc actgttcaat
    24721 attctgcttt cgctcgtcat tggcagccgt tacctgttta tcgccgactg gccgacaacg
    24781 cttgctggtc gcatttattc ctacgtaagc attatcggcc atttcagctt cctggtgttc
    24841 gccacctact tgctgatcct cttcccgctg acctttatcg tcggctccca gaggctgatg
    24901 aggtttttgt ccgtcattct ggcaacggcg ggaatgacgc tattactgat cgatagcgaa
    24961 gtctttactc gtttccatct ccatcttaat cccatcgtct ggcagttggt tatcaaccca
    25021 gacgaaaatg agatggcgcg cgactggcag ctgatgttca tcagcgtgcc ggttatttta
    25081 ttgcttgaac tggtgtttgc gacgtggagc tggcaaaagc tgcgcagcct gacgcgtcgt
    25141 cgacgcttcg cgcgcccgct ggccgcattc ttatttatcg cctttatcgc ctcgcatgtg
    25201 gtgtatatct gggccgatgc caacttctat cgcccgatca ccatgcagcg cgctaacctg
    25261 ccgctttcgt acccgatgac ggcgcgacgt ttccttgaga agcatggcct gcttgatgcg
    25321 caggagtatc aacgccgtct tattgagcaa ggtaatccag acgccgtttc cgtacagtat
    25381 ccgttaagcg aactgcgcta tcgcgatatg ggcaccgggc agaatgtgct gttgattact
    25441 gtcgatggcc tgaactactc acgcttcgag aagcagatgc ctgcgctggc aggttttgct
    25501 gagcaaaata tttcgttcac gcgccatatg agctccggca acactacaga caacggcatc
    25561 tttggcctgt tctatggcat ctcgccgagc tatatggacg gcattctgtc gacccgtacc
    25621 cctgcggcgt taattactgc gcttaatcag caaggctatc agctgggatt attctcgtca
    25681 gatggcttta ccagtccgct gtatcgccag gcattgttgt cagatttctc gatgccgagc
    25741 gtacgccccc aatccgacga gcagaccgcc acgcagtgga tcaactggct gggccgctac
    25801 gcacaagaag ataaccgctg gttctcgtgg gtctctttca atggcactaa cattgacgac
    25861 agcaatcagc aggcatttgc acggaaatat agccgggcgg caggcaatgt cgatgaccag
    25921 atcaaccgcg tgctcaatgc actgcttgat tctggcaaac tggacaatac ggttgtgatt
    25981 atcactgccg gtcggggtat tccgctgagc gaagacgaag aaacctttga ctggtcccac
    26041 ggtcatctgc aggtgccatt agtgattcac tggccaggca cgccggcgca gcgtattaat
    26101 gcgctgactg atcataccga tctgatgacg acgctgatgc aacgcctgct acatgtcagc
    26161 acacctgcca gcgaatattc gcaaggtcag gatttgttca accctcaacg ccgtcattac
    26221 tgggtcactg cagcggataa cgatacgctg gcaattacca ccccgaaaaa gacgctggtg
    26281 ctgaacaata acggtaaata ccgcacttac aacttacgtg gtgaaagagt gaaagatgaa
    26341 aaaccacagt taagtttgtt attgcaagtg ctgacagacg agaagcgttt tatcgctaac
    26401 tgattaatta taaatcagtt agcgaaatat cttacttgca atcggtgtgg aaaacggtag
    26461 tattagcagc cacgagtcgg cacgtagcgc agcctggtag cgcaccgtca tggggtgtcg
    26521 ggggtcggag gttcaaatcc tctcgtgccg accaaatcgc ccaccggata ccaccggaat
    26581 taacagtttt tagacacgaa aagcccgcta atccttgatt agcgggtttt tttatggttt
    26641 ttagtcaccg gacatcaccg gatttataag ctatcagccg gattttttag ttatacgctt
    26701 agttatacgg gcttgcgtat aactaaagcc gtataactaa aaccctgttt tttcgtgtgg
    26761 aatcatatac atatggcgag aaaaacaccc ccattaacca cggtgcagat caaagcagcc
    26821 agaccagcgg aaaaggaata caccctacag gacggcggag ggctttttct cctggtcaaa
    26881 ccgtccggat caaaactctg gcgattttcc tactaccggc catcggacaa aaaaagaata
    26941 ttgctgagtt ttggatcgct tgatgatgtt tccctggctg atgccagaaa acgccgtagc
    27001 gagtacagag cgttaatcag tgccggaact gacccgcagg gccatgagaa gaaaaaacga
    27061 gaggcagagg cccgaagaca agggaacacg ttcgaaaatg tggcggcggc atggtaccag
    27121 gtgaaaatca gccagaatct ggcccccaac acgattaaag acatctggcg ttcgctggat
    27181 aaatatgtat tcccgttcat cggcaacacg ccaatagata ccctcaccgc ccgaaggttc
    27241 gttgaagtgc ttacacccat caaggagcgc ggcaacctgg aaacactcaa gcgggtttta
    27301 cagcgcgtta atgaggtaat ggattacgcc gccaacagtg ggctgattga tgccaatccg
    27361 gctatgaatg tgcgtaaggc gtttccctca cctgtaaaaa aacatatgcc aacaatccgc
    27421 cccgaacagc tgccggagct tatgcaggct ttatcagtat cggcaacaga acggcagacc
    27481 agattactga ttgaatggca gttactgacc gtaacccgtc ccgccgaagc gtcatcaacg
    27541 cggtgggatg aaatcaacct ggacgcgaag caatggacga tacctgccgg acgcatgaag
    27601 atgcgcaggg atcacgttat cccgctttcc ggtcaggcta tggcggtgct ggaggccatg
    27661 aaaccaatca gccaccaccg caattacgtt ttcccaagtc tgaaagaccc acagcagccg
    27721 atgaacagcc agacagctaa cgcagcattg cggcgtatgg gattcgctgg cgtgctggtg
    27781 tctcatggat tacgcgccat attcagcaca gcagcgaacg aggaaggatt cgagccggac
    27841 gtaatagagg cggcacttgc ccacgtcgac accaacgaag ttagacgggc atacaaccgg
    27901 agcaactaca tagaaaaacg catcgtgctg atgcgctggt ggggcgaatt tgtcgaggct
    27961 gcggcgacgg gcgtaaccct cgccagtggt aaaaggggta tccgagccgt gtagctgtac
    28021 agaaaaccag taaaaactac gaaaaccatg taaaaccgtc gtataattgc atcaaattta
    28081 acgacaaggc cgtgaaacat gaaaccgtta agatgcaaaa aaatatcaga tgcaattgcg
    28141 acgggctgca actggccctg atggttcagc atgaattttg gtcaacctac gatccggagg
    28201 acagaacgac ggccccatca aaaaaacagg tagtagattt tctggtatcc cgtggcgctt
    28261 ccagaaatct ggcggtaagt attgataagg tcgtacgtcc ggcatctatg aagatcggag
    28321 gcaggcccaa aaaatggcgg taacaatcct ggaagcggca gaaatgccgc tttttttata
    28381 attccatttc aaatcatcaa tataaaaacg gtgtataccg tttaaaaacg gtgggaactg
    28441 tttttaccca tatccgatga tttaccgtat ttgtcaccgg aatacaccgg attcacaagg
    28501 taaatcacga tggaagcaat cagaaaaatt atctttcgcc aggaagtaaa aaaaattatc
    28561 cacatcaagg cagacagcac gctgcaaagc atgatcaacg ccggagaatt tccgcagggt
    28621 tttcgcgttg gtttacgccg tcgcggatgg tatgaggatg atgtgttggc ctggcagaaa
    28681 gaacgcgaac aggaagcacg cggaacggct gcttaacggg tatcacagat atgacaaaca
    28741 cgaaaaaagc ggccccgata tggagccgcc tttctgaaca attaacccgc tgcgccttat
    28801 gtgtatatga tcccaaacat aagcacgggg atgatagccg ctatcaggct ggtgggcaat
    28861 gc
//
LOCUS       DAJVUJ010000034        28686 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.034, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000034 DAJVUJ010000000
VERSION     DAJVUJ010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28686
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.034"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            44..811
                     /gene="hdhA"
                     /locus_tag="OQQ48_001402"
     CDS             44..811
                     /gene="hdhA"
                     /locus_tag="OQQ48_001402"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HCS1952228.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAISKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            1040..1618
                     /gene="uidR"
                     /locus_tag="OQQ48_001403"
     CDS             1040..1618
                     /gene="uidR"
                     /locus_tag="OQQ48_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HCS1952229.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERAREPIEGIHFVDYMVESIVSLTHEAFGQRALVV
                     EIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTSMTSRLLLDLTY
                     GVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            2006..3806
                     /gene="uidA"
                     /locus_tag="OQQ48_001404"
                     /pseudo
     CDS             2006..3806
                     /gene="uidA"
                     /locus_tag="OQQ48_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
     gene            3803..5176
                     /gene="uidB"
                     /locus_tag="OQQ48_001405"
     CDS             3803..5176
                     /gene="uidB"
                     /locus_tag="OQQ48_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HCS1952230.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVACVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYERYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARSGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            5215..6480
                     /gene="uidC"
                     /locus_tag="OQQ48_001406"
     CDS             5215..6480
                     /gene="uidC"
                     /locus_tag="OQQ48_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HCS1952231.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQRGLFDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPCENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAPIVTRGYESSKRDVRIEGTGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(6706..8214)
                     /locus_tag="OQQ48_001407"
     CDS             complement(6706..8214)
                     /locus_tag="OQQ48_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCS1952232.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITVAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(8315..9490)
                     /gene="manA"
                     /locus_tag="OQQ48_001408"
     CDS             complement(8315..9490)
                     /gene="manA"
                     /locus_tag="OQQ48_001408"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HCS1952233.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            9689..11335
                     /gene="fumB"
                     /locus_tag="OQQ48_001409"
     CDS             9689..11335
                     /gene="fumB"
                     /locus_tag="OQQ48_001409"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCS1952234.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGCDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            11478..12881
                     /gene="fumC"
                     /locus_tag="OQQ48_001410"
     CDS             11478..12881
                     /gene="fumC"
                     /locus_tag="OQQ48_001410"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HCS1952235.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(12878..13807)
                     /gene="tus"
                     /locus_tag="OQQ48_001411"
     CDS             complement(12878..13807)
                     /gene="tus"
                     /locus_tag="OQQ48_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HCS1952236.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRSLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(13883..15184)
                     /gene="rstB"
                     /locus_tag="OQQ48_001412"
     CDS             complement(13883..15184)
                     /gene="rstB"
                     /locus_tag="OQQ48_001412"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HCS1952237.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLYERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLVQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            complement(15188..15907)
                     /gene="rstA"
                     /locus_tag="OQQ48_001413"
     CDS             complement(15188..15907)
                     /gene="rstA"
                     /locus_tag="OQQ48_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HCS1952238.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGITICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     MPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRNVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            16033..16356
                     /locus_tag="OQQ48_001414"
     CDS             16033..16356
                     /locus_tag="OQQ48_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HCS1952239.1"
                     /translation="MKAALGVLVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHYIVAS
                     ERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWVLIIC
                     WIKLH"
     gene            complement(16353..17075)
                     /gene="folM"
                     /locus_tag="OQQ48_001415"
     CDS             complement(16353..17075)
                     /gene="folM"
                     /locus_tag="OQQ48_001415"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HCS1952240.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKLGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(17112..18494)
                     /locus_tag="OQQ48_001416"
     CDS             complement(17112..18494)
                     /locus_tag="OQQ48_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCS1952241.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLVLGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAASLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            complement(18680..19624)
                     /locus_tag="OQQ48_001417"
     CDS             complement(18680..19624)
                     /locus_tag="OQQ48_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCS1952242.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            20147..21679
                     /gene="pntA"
                     /locus_tag="OQQ48_001418"
     CDS             20147..21679
                     /gene="pntA"
                     /locus_tag="OQQ48_001418"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HCS1952243.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     TGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRRVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            21690..23078
                     /gene="pntB"
                     /locus_tag="OQQ48_001419"
     CDS             21690..23078
                     /gene="pntB"
                     /locus_tag="OQQ48_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HCS1952244.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQDVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(23103..24008)
                     /gene="tqsA"
                     /locus_tag="OQQ48_001420"
                     /pseudo
     CDS             complement(23103..24008)
                     /gene="tqsA"
                     /locus_tag="OQQ48_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
     gene            24185..25414
                     /locus_tag="OQQ48_001421"
     CDS             24185..25414
                     /locus_tag="OQQ48_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCS1952245.1"
                     /translation="MSRAMNKLSDTLLRKLHGKPAEKNTFYSDGGNLSVKYLTSGKLT
                     WYFIYRAGTGRETRPERIKLGSYPDLSLKSAREKAAQCRAWLAEGKNPRHELNYTVQE
                     ALKPVTVGDALTYWLEYYVKENRVDYIALKRRLNNHVIQQIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFVLQACKQALKFCRRRRYAISNVLDDLNVADVGKKPDISERVLSNK
                     ELGELLQALDKKIFSPYYVALIRLLIVFGARTVELRLSEIGEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTQLVEQNRHTGLLLGEVKQEASVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNALDMWTERLGI
                     LAGTHENVTTLPVARRK"
     gene            25781..25969
                     /locus_tag="OQQ48_001422"
     CDS             25781..25969
                     /locus_tag="OQQ48_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCS1952246.1"
                     /translation="MLSTDRFIREKECEKLTGLSRTCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            26027..27049
                     /locus_tag="OQQ48_001423"
     CDS             26027..27049
                     /locus_tag="OQQ48_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001429961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HCS1952247.1"
                     /translation="MKIEYTPERGRGFVRPGETEKQQNWGFSGIKKAAPKWSRLSEQI
                     TRCAVCVCDPKHKHGDDSRYQAGGQCNQSGSVRCHTCNERFSLYSLRNCSRAKAHGAN
                     LSDSCSIFLRRLFRAGDNVLVNSLSVIVLSCIAMRRNTLHHGADGDYSGSLALRRWRY
                     STSRFNAAVTNCPVLSPGIFTASTLFITSCGTLAATVCDFAFTAFVAMWCTPHKKQMQ
                     YAGKKISVQHLTCLTPGFKLVFNTLLAQGAETTKPGSALTLTGLLTTNDSESIEVAMR
                     NHTIHPQGRDSHNLNKYIWRFIALSTAQPRVITIEATSEQEARQQSPAGCVMVFAARI
                     RQGVGL"
     gene            27046..27276
                     /locus_tag="OQQ48_001424"
     CDS             27046..27276
                     /locus_tag="OQQ48_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1952248.1"
                     /translation="MSQEITLQQAAERAHQIEVICALAEDYPGMMTDSESGAIIGLLK
                     RLSGEVCVFLSDEQERRTLISNEKKCGGVHVQ"
     gene            27266..27490
                     /locus_tag="OQQ48_001425"
     CDS             27266..27490
                     /locus_tag="OQQ48_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1952249.1"
                     /translation="MCNKTTPDAAAAALTTLMHALIDISVIADRAHKHAARESECIFH
                     YLTFVQLKADQALDKAGKIIMADVQEVHHA"
     gene            27483..28061
                     /locus_tag="OQQ48_001426"
     CDS             27483..28061
                     /locus_tag="OQQ48_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000551752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1952250.1"
                     /translation="MHNLSISDLNSIQFDEKFTGQLLVNVENGRIVRNYHLPDGAIAG
                     SVEALLELAERARLIKPSTSHHDDDLHFTGCMVSHYENGVEVSCERLRDDCCFGTLPE
                     FIELLTSCGYQVIQGVNMRDDRFNSLKQEFSGVPDDAADALSSMPELIRAAFFLLSTR
                     EYKSTGLDVLNIVADYADFVTEVILRKTTDGD"
     gene            28063..28296
                     /locus_tag="OQQ48_001427"
     CDS             28063..28296
                     /locus_tag="OQQ48_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1952251.1"
                     /translation="MRDIYLETIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDILHDRYKEQHTGE"
     gene            28302..28601
                     /locus_tag="OQQ48_001428"
     CDS             28302..28601
                     /locus_tag="OQQ48_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCS1952252.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGTVTIKGVEERRITY
                     RREGYEYDCVMPVYQFRRDFSLVQAAPRSKPTSREKARANIQEIKKMLNVFRGKK"
BASE COUNT         7365 a         6814 c         7408 g         7099 t
ORIGIN      
        1 aggcggtgcc ttttattttt actgaagttc acaggaggtt tatgtgttta attctgacaa
       61 cctgagactc gacggaaaat gcgccatcat cacaggtgcg ggtgcaggta ttggtaaaga
      121 aatcgccatt acattcgcga cagctggcgc atctgtggtg gtcagtgata ttaacgccga
      181 cgcagctaac catgttgtag acgaaattca acaactgggg gggcaggcat ttgcctgccg
      241 ttgtgatatt acttccgaac aggaactctc tgcactggca gactttgcta tcagtaagct
      301 gggtaaagtt gatattctgg ttaacaacgc cggtggcggt ggacctaaac cgtttgatat
      361 gccaatggcg gattttcgcc gtgcttatga actgaatgtg ttttcttttt tccatctgtc
      421 acaacttgtt gcgccagaaa tggaaaaaaa tggcggtggc gttattctga ccatcacttc
      481 tatggcggca gaaaataaaa atataaacat gacttcctat gcatcatcta aagctgcggc
      541 cagtcatctg gtcagaaata tggcatttga cctgggtgaa aaaaatattc gggtgaatgg
      601 cattgcgccg ggggcaatat taaccgatgc cctgaaatcc gttattacac cagaaattga
      661 acaaaaaatg ttacagcaca cgccgatcag acgtctgggt caaccgcaag atattgctaa
      721 cgcggcgctg ttcctttgct cgcctgcagc cagctgggta agcggacaaa ttctcaccgt
      781 ctccggtggt ggggtacagg agctcaatta atacactaac gaaccggtaa acagccgtat
      841 gtgttgttta ccgggatagc gtgtgcaatt catcaacgtc tctgctaaac aactggcagc
      901 caaatcacgg ctattggtta accaatagct ggatgaaaag catacgaata gagtgtgcct
      961 tcgcactatt caacagcaat gataggcgct cacctgacaa cgcggtaaac tagttattca
     1021 cgctaactat aatggtttaa tgatggataa catgcagact gaagcacaac cgacacggac
     1081 ccggatcctc aatgctgcca gagagatttt ttcagaaaat ggatttcaca gtgcctcgat
     1141 gaaagccatc tgtaaatcct gcgccattag tcccgggacg ctttatcacc atttcatctc
     1201 caaagaagcc ttgattcagg cgattatctt acaggaccag gagagggcgc gggaaccgat
     1261 tgaagggatt cattttgttg actatatggt cgagtctatt gtctctctca cccatgaagc
     1321 ctttgggcaa cgggcgctgg tggtcgaaat tatggcggaa gggatgcgta acccacaggt
     1381 cgccgccatg cttaaaaata agcatatgac gatcactgaa tttgttgccc agcggatgcg
     1441 tgatgcccag caaaaaggcg agataagccc agacatcaac acgtcaatga cttcacgttt
     1501 actgctggat ctgacctacg gtgtactggc cgatatcgaa gcggaagatc tggcgcgtga
     1561 agcgtcgttt gctcagggat tacgcgcgat gattggcggt atcttaaccg catcctgatc
     1621 ctctcatttc gcgggctggt gataactgtg cccgcgtttc atactttaat ttccctgtac
     1681 aaaatacaat ctagccggga tcggagaatt cccccccaaa atattcaccg tagccatatg
     1741 tcatgagagt ttatcgttct caagatgctc gaacgagcgt tcgattgctt attttatggc
     1801 ttctgtcaac gctgttttaa agattaatgc gatctatatc acgctgtgcg tattgcagat
     1861 tttggttttt tgatcgcggt gtcagttctt tttatttcca tttctcttcc atgggtttct
     1921 cacagataac tgtgtgcaac acagaattgg ttaactaatc agattaaagg ttgaccagta
     1981 tttttatttg aatgaggagt cccttatgtt acgtcctgta gaaaccccaa cccgtgaaat
     2041 caaaaaactc gacggcctgt gggcattcag tctggatcgc gaaaactgtg gaattgatca
     2101 gcgttggtgg gaaagcgcgt tacaagaaag ccgggcaatt gctgtgccag gcagttttaa
     2161 cgatcagttc gccgatgcag atattcgtaa ttatgtgggc aacgtctggt atcagcgcga
     2221 agtctttata ccgaaaggtt gggcgggcca gcgtatcgtg ctgcgtttcg atgcggtcac
     2281 tcattacggc aaagtgtggg tcaataatca ggaagtgatg gagcatcagg gcggctatac
     2341 gccatttgaa gccgatgtca cgccgtatgt tattgccggg aaaagtgtac gtatcaccgt
     2401 ttgtgtgaac aacgaactga actggcagac tatcccgccg ggaatggtga ttaccgatga
     2461 aaacggcaag aaaaagcagt cttacttcca tgatttcttt aactatgccg ggatccatcg
     2521 cagcgtaatg gtctacacca cgccgaacac ctgggtggac gatatcaccg tggtgacgca
     2581 tgttgcgcaa gactgtaacc acgcgtctgt tgactggcag gtggtagcaa atggtgatgt
     2641 cagcgttgaa ctgcgtgatg cggatcagca ggtggttgca actggacaag gtaccagcgg
     2701 gactttgcaa gtggtgaatc cccacctctg gcaaccgggt gaaggttatc tctatgaact
     2761 gtgcgtcaca gccaaaagcc agacagagtg tgatatctac ccgctgcgcg tcggcatccg
     2821 gtcagtggca gtgaaggacg aacaaccaca aaccgttcta ctttactggc tttggtcgtc
     2881 atgaagatgc ggatttgcga ggcaaaggat tcgataacgt gctgatggtg cacgatcacg
     2941 cattaatgga ctggattggg gccaactcct accgaacctc gcattaccct tacgctgaag
     3001 agatgctcga gtgggcagat gaacatggca tcgtggtgat tgatgaaact gcagctgtcg
     3061 gctttaacct ctctttaggc attggtttcg aagcgggcaa caagccgaaa gaactgtaca
     3121 gcgaagaggc tgtcaacggg gaaactcagc aggcgcattt acaggcgatt aaagagctga
     3181 tagcgcgtga caaaaaccac ccaagcgtgg tgatgtggag tattgccaac gaaccggata
     3241 cccgtccgca aggtgcacga gaatatttcg cgccactggc ggaagcaacg cgtaaactcg
     3301 acccgacgcg tccgatcacc tgcgtcaatg taatgttctg cgacgctcac accgatacca
     3361 tcagcgatct ctttgatgtg ctgtgcctga accgttatta cggatggtat gtccaaagcg
     3421 gcgatttgga aacggcagag aaggtactgg aaaaagaact tctagcctgg caggagaaac
     3481 tgcatcagcc gattatcatc accgaatacg gcgtggatac gttagccggg ctgcactcaa
     3541 tgtacaccga catgtggagt gaagagtatc agtgtgcatg gctggatatg tatcaccgcg
     3601 tctttgatcg cgtcagcgcc ggcgtcggtg aacaggtatg gaatttcgcc gattttgcga
     3661 cctcgcaagg catattgcgc gttggcggta acaagaaggg gagcttcacc cgcgaccgca
     3721 aaccgaagtc ggcggctttt ctgctgcaaa aacgctggac tggcatgaac ttcggtgaaa
     3781 aaccgcagca gggaggcaaa caatgaatca acaactctcc tggcgcacca tcgtcggcta
     3841 cagcctcggt gacgtcgcca ataacttcgc cttcgcaatg ggggcgctct tcctgttgag
     3901 ttactacacc gacgtcgctt gcgtcggtgc cgctgcggcg ggcaccatgc tgttattggt
     3961 gcgggtattc gatgccttcg ccgacgtctt tgccggacga gtggtggaca gtgtgaatac
     4021 ccgctgggga aaattccgcc cgtttttact cttcggtact gcgccgttaa tgatcttcag
     4081 cgtgctggta ttctgggtac cgaccgactg gagccatagc agcaaagtag tgtatgcata
     4141 tttgacctac atgggcctcg ggctttgcta tagcctggtg aatattcctt atggttcact
     4201 tgctaccgcg atgacccaac aaccacaatc ccgcgcccgt ctgggcgcgg ctcgtgggat
     4261 tgccgcttca ttgacctttg tctgcctggc atttctgata gggccgagca ttaagaactc
     4321 cagcccggaa gagatggtgt cggtatacca tttctggacg attgtgctgg cgattgccgg
     4381 aatggtgctt tacttcatct gcttcaaatc gacgcgtgag aatgtggtac gtatcgttgc
     4441 gcagccgtca ttgaagatca gtctgcaaac cctgaaacgg aatcgcccgc tgtttatgtt
     4501 gtgcatcggt gcgctgtgtg tgctgatttc gacctttgcg gtcagcgcct cgtcgttgtt
     4561 ctacgagcgc tatgtgttaa atgataccgg gctgttcact gtgctggtac tggtgcaaaa
     4621 cttggttggt actgtggcat cggcaccgct ggtgccgggg atggtcgcga ggagcggtaa
     4681 aaagaatacc ttcctgattg gcgctttgct gggaacctgc ggttatctgc tattcttctg
     4741 ggtttccgtc tggtcactgc cggtggcgtt ggttgcgttg gccatcgctt caattggtca
     4801 gggcgttact atgactgtga tgtgggcact ggaagctgat accgtagaat acggtgaata
     4861 cctgaccggc gtgcgaattg aagggctcac ctattcacta ttctcattta cccgtaaatg
     4921 cggtcaggca atcggaggtt caattcctgc ctttattttg ggattaagcg gatatatcgc
     4981 caatcaggtg caaacgccgg aagttattat gggcatccgc acatcaattg ccttagtacc
     5041 ttgcggattt atgctactgg cattcgttat tatctggttt tatccgctca cggataaaaa
     5101 attcaaagaa atcgtggttg aaattgataa tcgtaaaaaa gtgcagcagc aattaatcag
     5161 cgatatcact aattaatatt caataaaaat aatcagaaca tcaaaggtgc aactatgaga
     5221 aaaatagtgg ccatggccgt tatttgcctg acggctgcct ctggccttac ctctgcttat
     5281 gcggcgcaac tggctgacga tgaagcggga ctacgcatca gactgaaaaa cgaattgcgc
     5341 agggcggata agcccagtgc tggcgcggga agagatattt acgcatgggt acagagagga
     5401 ttgttcgatt tcaatagtgg ttattattcc aatattattg gcgttgaagg cggggcatat
     5461 tatgtttata aattaggtgc tcgtgctgat atgagtaccc ggtggtatct tgatggtgat
     5521 aaaagttttg gctttgccct gggggcagta aaaataaaac cctgtgaaaa tagcctgctt
     5581 aaattaggtc gcttcgggac ggattatagt tatggtagct taccttatcg tattccgtta
     5641 atggctggca gttcgcaacg tacattaccg acagtttctg aaggagcatt aggttattgg
     5701 gctttaacac caaatattga tctgtgggga atgtggcgtt cacgagtatt tttatggact
     5761 gattcaacaa ccggtattcg tgatgaaggg gtgtataaca gccagacggg aaaatacgat
     5821 aaacatcgcg cacgttcttt tttagccgcc agttggcatg atgataccag tcgctattct
     5881 ctgggggcat cggtacagaa agatgtttcc aatcagatac aaagtattct cgagaaaagc
     5941 ataccgctcg acccgaatta tacgttgaaa ggggagttgc tcggctttta cgcgcagctc
     6001 gaaggtttaa gtcgtaatac cagccagccc aatgaaacgg cgttggttag tggacaattg
     6061 acctggaatg cgccgtgggg aagcgtattt ggcagtggtg gttatttgcg ccatgcaatg
     6121 aatggtgccg tggtggatac cgacattggc tatccctttt cattaagtct tgatcgtaac
     6181 cgtgaaggaa tgcagtcctg gcaattgggc gtcaactatc gtttaacgcc gcaatttaca
     6241 ctgacatttg caccgattgt gactcgcggc tatgaatcca gtaaacgaga tgtgcggatt
     6301 gaaggcacgg gtatcttagg tggtatgaac tatcgggtca gcgaagggcc gttacaaggg
     6361 atgaatttct ttcttgctgc cgataaaggg cgggaaaagc gcgatggcag tacgctgggc
     6421 gatcgcctga attactggga tgtgaaaatg agtattcagt atgactttat gctgaagtaa
     6481 gaaataacgc cggagagaaa aacctccggc gtttcagatt gttgacaaag tgcgcgtttt
     6541 ttatgccgga tgcggcgtaa acgccttatc cagcctacaa aaactcataa attcaaagta
     6601 ttgcaggaaa aggtaggcct gataagcgta gcgcatcagg caatctctgg tttgttttca
     6661 gatgaaaacg ccgaagtgaa aattctccgg cgttttggcc gtgaattact gctgcggaat
     6721 tgccggtaca gccggaacgt taagagctgg catcgcaaac atgccaacaa aatcttctaa
     6781 cgacattttc tgcccattta acgttatctg accgttagta tattgcaggc tggtggtgat
     6841 ggtattgtcc tgcaaggtgg tcagacggaa catctgcccc attgctgatg caccttcaac
     6901 ttgctgtttc gccagttttt tcgcttgatc ttcctgataa ccttccagct tcgctacctg
     6961 agtcataaac tcagttgcca tatccaccgg aatggtcagt ttcgcatcca gagatttaac
     7021 cgaacgatct acttcctgcg ccagcgtttg cggcgcttct ttagtcgttg ccggatcttt
     7081 caggaacagc gacagattca gcgcactttc accctgactg tttttccagc ttagcggcgc
     7141 gacagtaatc accggatcgc ctttcaacat cagcggcagg gcgctaaaga aggcttccgt
     7201 cactttctcc tgataaagtt cgggattgtt ggcaatttct ggctgtgcca gcagcgcctg
     7261 agtttgcgcg ttatattgct ggctgaactg atgccaggct tcaccatcaa tctggccgac
     7321 ttttaaagtc agcttgccgc tgcccagatc ctgattctgt accttcaggc tgtttagcga
     7381 gtaatccagt tggctattga tcgttttacc gtcattgacc agatccgatt taccgctgat
     7441 ctccatgcct tccagcagtg ccagttcttt gccttccact gaaatggtca ttttttccag
     7501 tgacagtttt tggtttccta cgcgctcacc aaaacttgcc agcgtgctgg aaccgtcggt
     7561 tttcagatta ttaaaggtca actgcacttt ctggttgtat tcgttaactg cgtctatccg
     7621 accactttgc gcctccccgg aaagggagat ggctttgccg tctctgtcag catttaactg
     7681 gaactcgccg ccgctaaagg cgactttttc atccttttgc tcgtaattca gtggcttgag
     7741 cgaaatatcg gaactggaat caccgctgta accaatgcgc gagttaatct caaaaggcgt
     7801 ttcacctttt gccatatcaa acagtggttt gcttacttcg ttattaacca gcgtggtttg
     7861 aattgatgcc atcgacggga tcaggttcag ttttttaagc tgggcaagcg ggaagggacc
     7921 atgatcaacc gattcgttga agatgacgct ctgaccgctt ttaatccacg gattttcttt
     7981 cccggcaatg ggtttcacca acagttgcaa ctgactgctg aatacgccgc gatgatagtt
     8041 ttgataactc acttccaggt tggattcagg agctgtcagt tttagttgcg cgttcgcctg
     8101 cgcgaccatg tcttcgagat gggtttcaat cttcttgcct gtataccatg cgccgcctgt
     8161 ccagactacg cctagcgcaa caatgacgcc taccgctacc agcgatttat tcataatgat
     8221 tatccataaa atgaaatcag gcggactggc cgcctgaagg tgttataagc ctttaataag
     8281 cttagcaaga gatgttaatt ttttcagtaa gctcttacag cttgttgtaa acacgcgcta
     8341 aacggccgtg gcctttgaca gtcaccggtg attcgttggc ggcaataaac gctgattcac
     8401 ccggtttaag ctgtaactgc tgagaacctt tccacaacgt tgcatcgcct tcgacgcaga
     8461 acaaaatggc ggcactctgc tggctaatgg tggtttcttt atcactaagg tcatgcagcg
     8521 agaaggcaaa atcatccact ggaatcggga agtccagttc tgcaccttgt ttcaccggct
     8581 gggtcaacaa ctggttagcc ggtttggctt cgaatttcac attggcaacc agttccggaa
     8641 tatcaatgta cttaggcgtc agacccgcac gcagcacgtt atcggagttc gccatcactt
     8701 ccagcgccac gccttgcagg taagcgtgcg gtgtttcagc gaacaggaac atcgcttcgc
     8761 caggattcaa tttcaccaca ttcagtaaca gcggggagaa caggccgctg tcttccgggt
     8821 aaaattcaga aattaaacga atcgtttgcc acggttcacc ctgctgactg tcgagcgccg
     8881 attttaaaat cgccagcgcg cgggattttt cttcaccctg catattcaac aggctggcga
     8941 acagttcgct taaacgttcg gcattaggct gttgtaaaaa gtgagcaatt gccggatgtg
     9001 cacctgcgac tggctggagt agggagacaa tctcggaaaa ctcacgaaac gcgttcatcg
     9061 caaggaaagg cgtcagcgca aaaaccagct ccggcttgtg gttaggatct ttatagttac
     9121 gctcggcggc atccatcggg atacctgcgg cattttcttt ggcaaaaccg atttcagaat
     9181 tgcgtttgtt tggatgaacc tgaatggaga gcggctgtgc tgcgcataat actttgaaca
     9241 ggaaaggcag ttcgccaaag cgtttggcaa cggcctctcc gagcagagtc gatttatcac
     9301 tctcaatcac atcacgcagt gaaacgatat ctccggcggc attctgcact cgtgaactgc
     9361 ttttcggatg tgcgcccatc cacagctcgg ccatcggctg gctggacgga ttttccatac
     9421 cataaagttc agtcaacgcc gttttgctgc cccaggcata gttttgcact gagttaatga
     9481 gtttttgcat gatcaatccc tgttttaatg tggaaattaa tcccactatt aaagcaagaa
     9541 tcctacggga agtaacctgg agccgcaaaa agtcgtacta gtctcagttt ttgttaaaaa
     9601 agtgtgtagg atattgttac tcgcttttaa cagggcaacg gaacacccgc ccagagcata
     9661 accaaaccag gcagtaagtg agagaacaat gtcaaacaaa ccctttcatt atcaggctcc
     9721 ttttccactc aaaaaagatg atactgagta ttacctgcta accagcgaac acgttagcgt
     9781 atctgaattt gaagggcagg agattttgaa agtcgcaccc gaagcgttaa ctctgttggc
     9841 gcgtcaggcg tttcatgatg cgtcatttat gctgcgtccg gctcaccaac aacaggtggc
     9901 cgacattctg cgtgacccgg aagccagcga aaatgataaa tatgtggcgc tgcaattcct
     9961 gcgcaactcc gacatcgcgg cgaaaggtgt tctgccaacc tgtcaggata ccggcactgc
    10021 gattattgtt ggtaaaaaag ggcagcgtgt atggaccggt ggctgtgatg aagcggcgct
    10081 ggcgcgcggt gtctataaca cttatatcga agataatttg cgctactcgc aaaacgcgcc
    10141 gctggatatg tataaagagg tgaataccgg caccaatctg ccagcgcaga tcgatcttta
    10201 tgccgttgat ggcgacgagt acaaattcct ctgtatcgcc aaaggtggtg gttcggcaaa
    10261 caagacgtat ctctatcagg aaaccaaagc gttactaacg ccggggaaac tgaaaaatta
    10321 cctggttgag aagatgcgca cgctgggtac ggcggcctgt cctccgtatc atattgcgtt
    10381 cgtaattggt ggaacttctg cagaaactaa ccttaaaaca gtgaaactgg cttccgctaa
    10441 atactatgac gaactgccaa cggaagggaa tgagcacggt caggcattcc gcgatgtgga
    10501 actggaaaaa gaattgctga tcgaagcgca aaatctcggt ctgggtgcgc agtttggtgg
    10561 taaatacttc gctcacgaca tacgcgtgat tcgcctgcca cgtcacggcg catcctgtcc
    10621 ggttggtatg ggtgtttcct gctctgctga ccgtaatatc aaagcgaaga tcaaccgtca
    10681 ggggatctgg atcgaaaaac tggaacataa tccaggtaaa tatatccctg aagagctgcg
    10741 taaagcggga gaaggcgaag cggtgcgcgt tgaccttaac cgtccgatga aagagatcct
    10801 cgcacagttg tcgcagtatc ccgtttctac acgcttatcg cttaacggca cgattatcgt
    10861 cggtcgtgat attgctcacg ccaaactgaa agagcggatg gataacggtg aagggctgcc
    10921 gcagtacatt aaagatcatc cgatttacta cgcgggtccg gccaaaacgc cggaaggtta
    10981 tgcctccggt tctcttggcc caacgaccgc cggacggatg gattcttatg tcgatcaact
    11041 gcaagcgcag ggcggaagta tgatcatgct ggcgaaaggc aaccgcagcc agcaggtgac
    11101 ggatgcctgt aaaaaacacg gcggcttcta ccttggcagt atcggtggtc cggccgctgt
    11161 attggcgcag ggaagtatta agagcctgga atgtgttgaa tatccggaac tgggaatgga
    11221 agccatctgg aaaattgaag tggaagattt cccggcgttt atccttgtgg atgataaagg
    11281 aaatgacttc ttccagcaga tacaactcac acagtgcact cgctgtgtga aataaacaga
    11341 gccgcccttc ggggcggttt ttttacatgg cacgaaagac caaacacttg ttatcaaata
    11401 gtaaataata agtgagctaa aagttgctta gcgaaagcaa aacagaaaga aaaaattaaa
    11461 caggtgagga gcaggccatg aatacagtac gcagcgaaaa agattcgatg ggggcgattg
    11521 atgtcccggc agataagctg tggggcgcac aaactcaacg ctcgctggag catttccgca
    11581 tttcgacgga gaaaatgccc acctcactga ttcatgcgct ggcgctaacc aagcgcgcag
    11641 cggcaaaagt taatgaagat ttaggcttgt tgtctgaaga gaaagcgagc gccattcggc
    11701 aggcggcgga tgaagtactg gcaggacagc atgacgacga attcccgctg gctatctggc
    11761 agaccggctc cggcacgcaa agtaacatga acatgaacga agtgctggct aaccgggcca
    11821 gtgaattact cggcggcgtg cgcgggatgg aacgtaaagt tcaccctaac gacgacgtga
    11881 acaaaagcca aagttccaac gatgtctttc cgacggcgat gcacgttgcg gcgctgctgg
    11941 cgctgcgcaa gcaactcatt ccgcagctta aaaccctgac gcagacactg agtgaaaaat
    12001 cgcgtgcatt tgccgatatc gtcaaaatcg gtcgaaccca cttgcaggac gccacgccgc
    12061 taacactagg gcaggagatt tccggctggg tagcgatgct cgagcataat ctcaaacata
    12121 tcgaatacag cctgcctcac gtagcggaac tggctcttgg cggtacagcg gtgggtactg
    12181 gactaaatac ccatccggag tatgcgcgtc gcgtagcaga tgaactggca gtcattacct
    12241 gtgcaccgtt tgttaccgcg ccgaacaaat ttgaagcgct tgcgacctgt gatgccctgg
    12301 ttcaggcgca cggcgcgttg aaagggttgg ctgcgtcact gatgaaaatc gccaatgatg
    12361 tccgctggct ggcctctggc ccgcgctgcg gaattggtga aatctcaatc ccggaaaatg
    12421 agccgggcag ctcaatcatg ccggggaaag tgaacccaac ccagtgtgaa gcattaacca
    12481 tgctctgctg tcaggtaatg gggaacgacg tggcgatcaa catgggtggc gcttccggta
    12541 actttgaact gaacgtcttc cgtccgatgg tgatccataa tttcctgcaa tcggtgcgct
    12601 tgctggcaga tggcatggaa agttttaaca aacactgcgc agtgggtatt gaaccgaatc
    12661 gtgagcgaat caatcaatta ctcaatgaat cgctgatgct ggtgactgcg cttaacaccc
    12721 acattggtta tgacaaagcc gcggagatcg ccaaaaaagc gcataaagaa gggctgacct
    12781 taaaagctgc ggcccttgcg ctggggtatc ttagcgaagc cgagtttgac agctgggtac
    12841 ggccagaaca gatggtcggc agtatgaaag ccgggcgtta atctgcaaca tacaggtgca
    12901 gccgtggaat gatcaaacga agcggctgcg cctgaggttt ataacggtgc tgcacattgt
    12961 cggcatcgta atttaacaac tcaccaacgt ccggcacgcc cgcaccatta tcccgattaa
    13021 tcagtgcaat cagcggtgta gggcaggcgt gttgcacttg tttttgatct cctttgtacc
    13081 agacgcgggc aatcggctgc accttcaccg gacgtttgat ttttaacttc gcgttctgtg
    13141 gcagggcagc gatatcctga tactctcgct ccagttttct ttgccactcc tcgcgcgtcc
    13201 acggtgcgac actgcgtggt gatttcaggc ttttttccag ctgtgccagg acttcatcac
    13261 gatgcaaatt tttaatgata tgtttattag cccagccaaa acgtaaagtg gcggggtcgt
    13321 gcagaacggt gagcgagcgg taagcattaa gggtgatcag ccccggcaaa tgacgatgca
    13381 cccattcaaa acgtgccgcg gtggggagtt ctgactcaac cgtgacgata tgctcgaacg
    13441 tggtcttgag tttattgatg tgctgaatat gactgaccaa cgctgcttgc gaaaggttat
    13501 cgacctggta acacaacacg ccaggcagac ggacagcggc tttgctgctg cgattttcgg
    13561 actgttgttg aataaataaa tggcggaaat gacgcaacgc cagcgactgc gcgtcgttgc
    13621 cgagatgttg tttcacctca atacgattaa gcggattatg ctcatcctct ttttttacct
    13681 ccggcaaaga gaacacgcgg gcaaccaata gcttgtgttg ctcaagatga gcggcaaatg
    13741 cagccagctc ttgttccatc tggcgaaagg tagtgttgag tcggtctacg agatcgtaac
    13801 gcgccataat attaaccact ttagttacaa catccttaat ttatagcaca atggtggtga
    13861 cttgaccatc gtgcgcgtag tgtcaggcag aggtaaattg cgggatgtta tgccataacg
    13921 gccagctaaa cgagaagcga gcaccaccca gttcgctggt gtcacaatta accgtaccgc
    13981 ccattgccag tgctatagag tggacaattg ccagccccag cccgcagccg ccggttgagc
    14041 gatcccggct gggatcgagg cgaacaaaag gttcaaagat atgttcgcgg ttttctgggg
    14101 caatccctgg gccatcatcc tcaacaatta atgtcgctct attccccgac agtagcaggc
    14161 tggtttcaac cgttgaatgg cagtaccgca gggcgttatt gagcaaatta tccagcacgc
    14221 gctccattaa gcgcatatcc aacgccgcat aatggccttg cacgagcgtt tttatccgta
    14281 ccgttttatc gggcgttact gcctgaatat ctgccagatg cgttgacagc cacaacggca
    14341 ggtctggttc gctaagatga agctcgtttt gtgggcgatc gagtcgggca taagtcagca
    14401 gctcttcaat taaagcttca agttgactga tatcacgatt caacgcctgg gactcggcgg
    14461 cgctcaggtt atcgctcatc tccagtcgat aacgcaggcg cactaacggt gttcgcagtt
    14521 cgtgagcgat accgtcaata agctgttttt tgctggcaat caaggcgttg atattgtccg
    14581 ccatctggtt aaatgcgata ccaagtcgtt caaagctcga accctcatca aagtggatac
    14641 gctcatagag atgcccatcg ccaaatcgtt gcgccgctgc ttccagtttt aacatatcct
    14701 gccagtgcgg acgcatccag ataaacaccg gaaaggcgag ggaaatagca ataaaagcga
    14761 tcagggcgat atccagcaat cgcatctgat ggagataata aagataagga acaggaccaa
    14821 ctgccagcac gtagtggctg cgcgggatac gctgcaaaaa cgtgtactga tcgtccaggg
    14881 cgacaatttc gccgccacgc agtcggtgca tggaaatatc atcaagatgg tatttactca
    14941 gtggctcgac acgcagatcg aaagagagat ttaaatccat ctctttcagc gtcttacccc
    15001 agtcgtgtgg ggggatctca cgcaattcgc tgcgcatcag atacagcgaa ctgttcatca
    15061 aatcatccag cgactgtttg cccgcgcgtt cggcggtaaa tttgtacacc aacccaacca
    15121 gcagagacat cacaaggaag cagacaaaca ataacaggta aaactggata aacagttttt
    15181 tcatcgctta ttcccatgca tgaggcgcaa aaagatagcc tttgttacgc acagttttaa
    15241 tgcgataagg ttctgcggcg ttatcgagca gtttttttct taaccgcgaa atagccacgt
    15301 ccacgttacg atccagtccg tcataactga cgccgcgtaa atttttcagc aatgcatcgc
    15361 ggtccatgat ttgcccggca tgggtagcta attcccacaa taattcgaaa tcagctgtcg
    15421 agagcgagat ttcagtgtta gccagggtga ctacgcggtt gatgggatcg atggtcaacg
    15481 tgccgaaatg cagggctttg tagggagtca gagacgtttc ctgaagacct ttggtcagtg
    15541 tggcttgctc attctgacgc aaatgcaaac gtaaacgcgc tagcaaaaca gcagggggca
    15601 tcgttttgag aatatagtcg caggcaccca tttccagtgc caggatgtgg ttcatatcgc
    15661 tatcgagaga ggttagaaga acaatcggtc cagaccactt tgcgcgtaaa tcacgacaaa
    15721 tggtaatgcc gtccttgcct ggtagcatga tgtcgagtaa caccaaatcc ggattttctc
    15781 gcaaaatggt ttcttcggcc tggtcgccgc gcggctctac ggtaacctgc atatcatgtt
    15841 ttgccaggta cgcggcaatc agtgaaccga cttccgcatc atcttccaca aatacgatag
    15901 tgttcataac attcacggta gatataaaaa cgtcacaata caccgcgctg tttttactta
    15961 ctatcaatcg tttctaaaca agttttcatc aggtattcta ctgatgactt gttatggaag
    16021 tgttaaggta aaatcaaagc cgcgctcggt gtgctggtag tgctgttgat tggtgtttta
    16081 gcaaaaacga aaaattatta tatcgccggg ctgattccac ttttcccgac ctttgcgctt
    16141 atcgcgcatt atattgttgc cagcgaacgc ggcattgaag ccttacgcgc aaccatcatt
    16201 tttagtatgt ggtcgattat tccctatttt gtttacctgg tgtcgctgtg gtatttcacc
    16261 ggaatgatgc gcttgcccgc cgcttttgtt ggttctgttg cgtgttgggg gataagcgca
    16321 tgggtgttga ttatatgctg gataaagctg cattaacgca gatgacgacc gccatcaagt
    16381 gggaaactgc gtccggtgac aaagcaactg gtaagtaagt aatcgaccag gtcgatcact
    16441 tctttctcgc caggcgcggt tttcatcagt gatttattca gcgcctgttg tcgatattcg
    16501 gcatcatcat gttcattaaa caggatcagc gatggagcaa tagaattcac tttcacttcc
    16561 ggtgccagct tgcgggcaaa cgagcgggtc atattatcca gtgccgcttt gcttgcagca
    16621 tacgcaatat gcttgtcgct accgcgctcc accacataat cggtaaagtg aatgatatcg
    16681 ctggcggcgt gtccgtgccc acgcagtaat ctttccagcg catggttgag caggtatggg
    16741 gtattaacgt ggatctgcat catgcaagcc agtacgtcgg ccagtggcgc acccagtttt
    16801 tccgccatcc acgcactggc gttatgcaaa atagcacgca gaccatgggt gctttttagt
    16861 acttcatcgg caaacgccat cacaccgtcg ttggtcgaaa aatcagcctg aatacactgc
    16921 gcacctgcct taatcagtcc atcaatggct ggatagtgcg tccgatagct gacaatcacc
    16981 ggttgctttt gattaatgaa atgccaggcg agggcgaggc cgatgcgacg acctccgcca
    17041 gtaattaata ttggcaaggg ctgggcttta cccatcgtta tctcctttgc tatccaacga
    17101 tgggatgaac gttatcccac cagcatccag gtcgccggaa ctgaggcaat taacagcatg
    17161 ccaatcagca ccatttcctg acggttcagt acattatcat gtgtatgcgt tttgcgcgca
    17221 tacaggaaaa ccagcagccc cggcgcatac agaacaacgg ataacagcaa gtgcattgga
    17281 ccagaggcgt acaataacca taagccataa atgcaggcac cgacacctac cgctttatgc
    17341 agtggacggg ttgcgatttt cagcaagaac gcgccgacca ggaaataggg caccagaatc
    17401 atttctgagg cgatggtcag caacgtgttg taatcggaac cggtcagcca gatgagtacc
    17461 agacaaattt gcacgcagat gttggtcaac cacagtgagg cggatggcgc agcttgtgcg
    17521 ttctggcgcg caaaaatgcg tgggaatgct ttatgagtgg cagccaggaa cggaacttct
    17581 gccgccatga tggtccagct taagtacgcg ccgcaaacgg aaacgatcaa actggcagcg
    17641 atgatgattt cgccccatgg tcccatcatt tccaccatca gaccggccat tgacgggtta
    17701 cgaatttcag ccagttcagg acgtgccacc acacccagcg aaagcagcgt taccagcaag
    17761 taaacgccca gagcggagag aaccgccagc agtgtcgctt tgccaacatc acgtttatta
    17821 cgcgcacgcg cagaaacaac cacggcacct tccacaccaa tgaaaaccca cagggtgatc
    17881 agcatggtgt ttttcacctg ttcccaaacg ggtacgccaa gtacaagtcc ggtgaagtcg
    17941 agcttgaagg tatccagttt gaacatcatc atcgccagca caacaaacag acccagcggc
    18001 aacaattttg ccagtgtcgc caccaggtta atgctggcag cggtttgcac cccgcgcagg
    18061 atcaaaaagt gaacaatcca caataaagcc gatgcaccga cgatcgattg ccaggtatta
    18121 ccatcgccga acaggcgcaa ttccggcgtg tccgtaaaaa agcttaacgc ggaaaaaacg
    18181 atcaccagat aggagacgtt ggcgatgact gcgcacagcc agtatcccca tgcggaacaa
    18241 aagccgatta gctcgccaaa cccttcgcgg gcataggtaa agataccgcc gtcaagttcg
    18301 ggacgaatgc gcgtgaggat cagcatggca aaggccagca ataaaatgcc agcgccagta
    18361 ataccccagc cgatgagcag tgctgccggg ctggcaactg ccgccatatt ttgcggcaga
    18421 ctgaaaacac ccgcgcccag cattgagctt aataccagcg cggtgagtgc gctcagtccc
    18481 agtttctttt ccatcgatat ccagtgatta aaacgtgata agaagaataa atattagggc
    18541 aaaacgcata aaaatggagt ttgccactga agcgcgggat tttacgaaga gatgtgtctg
    18601 catgcaatgg tcgcggggaa atttgtgact aaaaatgcga aaggcggcat caagccgcct
    18661 tataggagta actgaatagt tatttataca gatctgcgct gacggtcagg ttattaccac
    18721 gttcctgcca ctggcgggtg atgtggtaat acttagcacc tttcttcgcg gcacgtttcg
    18781 caacctgata ggagacttcg gtcatgttgc cgtagttgcc agagaatttg atgctatcga
    18841 acgggaccat catcgctgcg gtcgctttgt tcagttcttc gactttagtg ccatccggga
    18901 gcgtgacggt gtaacgcccg ccttttgatg actgggtttc aaagaaacga ccgacttcag
    18961 aacttggtga tgcggtagtc gcaacacctg ggatctcaac tttcttcgcg gcttcgccac
    19021 cggcagccag agctgcacgt cctgcttcgg aatctgccgg gatcacatcc gggctctgga
    19081 cgatacgttt cttagcatct tttttataga tgaatgcagt aatacgctgg ttgccgccct
    19141 ggttggcatc gatttgacga acgatgtaga aagagtaggc acctttcgct tttgccgctt
    19201 tggtgatggc atcattgact tctggctggc tacgatagaa gccctggacg gtgaccgtgt
    19261 caaacggttc aatcagaaca gcctgatctt tcggcagttc gacaacaccg ttaattacgc
    19321 gattacttgt ttcttctgct ttttcagcat cggctttata gaggtcagcg accacacgcc
    19381 agttaccgct gttaccaaaa tcagaagtgt cgacaacata aaaagaggcg gcaccttctt
    19441 tatctgcgcg acgagaaacg gctttcaccg cttcgccaat agcattaaaa cgaccggtaa
    19501 ccactacacg gtcaaaaggt ttaaccgctg ccgcttgctc cggtgtcagt tctgttgctg
    19561 cgttaacgga gaatgccgta gcagaaagca gtgccgacgc caggagggtg ttcttaagct
    19621 tcataaaata atccttcgcc ttgcgcaaac caggtactgg tattgttatt aacgagaaac
    19681 gtggctgatt attgcattta aacggtgtaa ctgtctgcgt catttttcat atcacattcc
    19741 ttaagccaat tttaatcctg ctcaaatgac cgtctatgct taaaaaacag ccgtatcagc
    19801 atcattacta ctgaagcaac tgaattgtat aagttaattt aatgttaagt agtgaatcgt
    19861 gccggggcga tgtctcgttt tacccgaccg tcgaagacaa ttatcagtct ttatccggcg
    19921 ttctaaggtg tttatcccac tatcacggct gaatcgttaa tattttgcga gttcacgccg
    19981 aaatactgat ttttggcgct agatcacagg tataattttc agtacgttat agggcgtttg
    20041 ttactaattt attttaacgg agtaacattt agctcgtaca tgagcggctt gtgtggctcc
    20101 tgacacaggc aaaccatcat caataaaacc gatggaaggg aatatcatgc gaattggcat
    20161 accaagagaa cggttaacca atgaaacccg tgttgcagca acgccaaaaa cagtggaaca
    20221 gctgctgaaa ctgggtttta ccgtcgcggt agagagcggc gcgggtcaac tggcaagttt
    20281 tgacgataaa gcgtttgtgc aagcgggcgc tgaaattgta gaagggaata gcgtctggca
    20341 gtcagagatc attctgaagg tcaatgcgcc gttagatgat gaaattgcgt tactgaatcc
    20401 tgggacaacg ctggtgagtt ttatctggcc tgcgcagaat ccggaattaa tgcaaaaact
    20461 tgcggaacgt aacgtgaccg tgatggcgat ggattctgtg ccgcgtatct cacgcgcaca
    20521 atcgctggac gcactaagct cgatggcgaa catcgccggt tatcgcgcca ttgttgaagc
    20581 ggcacatgaa tttgggcgct tctttaccgg gcaaattact gcggccggga aagtgccacc
    20641 ggcaaaagtg atggtgattg gtgcgggtgt tgcaggtctg gccgccattg gcgcagcaaa
    20701 cagtctcggc gcgattgtgc gtgcattcga cacccgtccg gaagtgaaag aacaagttca
    20761 aagtatgggc gcggaattcc tcgagctgga ttttaaagag gaagcgggca gcggcgatgg
    20821 ctatgccaaa gtgatgtcgg acgcgttcat taaagcagaa atggaactct ttgccgccca
    20881 ggcaaaagag gtcgatatca ttgtcaccac cgcgcttatt ccaggcaaac cagcgccgaa
    20941 gctaattacc cgtgaaatgg ttgactccat gaagacgggc agtgtgattg tcgacctggc
    21001 agcccaaaac ggtggcaact gtgaatacac cgtgccgggt gaaatcttca ctacggaaaa
    21061 tggtgtcaaa gtgattggtt ataccgatct tccgggccgt ctgccgacgc aatcctcgca
    21121 gctttacggt actaacctcg ttaatctgct gaaactgttg tgcaaagaga aagacggcaa
    21181 tatcactgtt gattttgatg atgtggtgat tcgccgcgtg accgtgatcc gtgcgggcga
    21241 aattacctgg ccggcaccgc cgattcaggt atcagctcag ccgcaggcgg cacaaaaagc
    21301 ggcaccggaa gtgaaaactg aggaaaaatg tgcctgctca ccgtggcgta aatacgcgtt
    21361 gatggcgctg gcaatcatcc ttttcggttg gctggcaagc gttgcgccga aagagtttct
    21421 tgggcacttc accgttttcg cgctggcctg cgttgtcggt tattacgtgg tgtggaatgt
    21481 atcgcacgcg ctgcatacac cgttgatgtc ggtcaccaac gcgatttcag ggattattgt
    21541 tgtcggagca ctgttgcaga ttggccaggg cggctgggtt agcttcctta gttttatcgc
    21601 ggtgcttata gccagcatta atattttcgg tggcttcacc gtgactcagc gcatgctgaa
    21661 aatgttccgc aaaaattaag gggtaacata tgtctggagg attagttaca gctgcataca
    21721 ttgttgccgc gatcctgttt atcttcagtc tggccggtct ttcgaaacat gaaacgtctc
    21781 gccagggtaa caacttcggt atcgcaggga tggcgattgc gttaatcgca accatttttg
    21841 gaccggatac gggtaatgtt ggctggatct tgctggcgat ggtcattggt ggggcaattg
    21901 gtatccgtct ggcgaagaaa gttgaaatga ccgaaatgcc agaactggtg gcgatcctgc
    21961 acagcttcgt gggtctggcg gcagtgctgg ttggctttaa cagctatctg catcatgacg
    22021 cgggaatggc accgattctg gtcaatattc acctgacgga agtgttcctc ggtatcttca
    22081 tcggggcggt aacgttcacg ggttcggtgg tggcgttcgg caaactgtgt ggcaagattt
    22141 cgtctaaacc gttgatgctg ccaaaccgtc acaaaatgaa cctggcggct ctggtcgttt
    22201 ccttcctgct gctgattgta tttgttcgca cggacagcgt cggcctgcaa gtgctggcat
    22261 tgctaataat gaccgccatt gcgctggtat tcggctggca tttagtcgcc tccatcggtg
    22321 gtgcagatat gccagtggtg gtgtcgatgc tgaactcgta ctccggctgg gcggctgcgg
    22381 ctgcgggctt tatgctcagc aacgacctgc tgattgtgac cggtgcgctg gtcggttctt
    22441 cgggggctat cctttcttac attatgtgta aggcgatgaa ccgttccttt atcagcgtta
    22501 ttgcgggtgg tttcggcacc gacggctctt ctactggcga tgatcaggaa gtgggcgagc
    22561 accgcgaaat caccgcagaa gagacagcgg aactgctgaa aaactcccat tcagtgatca
    22621 ttactccggg gtacggcatg gcagtcgcgc aggcgcaata tcctgtcgct gaaattaccg
    22681 agaaactgcg cgctcgtggt atcaacgtgc gtttcggtat ccacccggtt gcggggcgtt
    22741 tgcctggaca tatgaacgta ttgctggctg aagcaaaagt accgtatgac atcgtgctgg
    22801 aaatggacga gatcaacgat gactttgctg ataccgatac cgtactggtg attggtgcta
    22861 acgatacggt taacccggcg gcgcaggatg atccgaagag tcctattgct ggtatgcctg
    22921 tgctggaagt gtggaaagcg caggacgtga ttgtctttaa acgttcgatg aacactggct
    22981 atgctggtgt gcaaaacccg ctgttcttca aggaaaacac ccacatgctg tttggtgacg
    23041 ccaaagccag cgtggatgca atcctgaaag ctctgtaacc ctgacggcct ctgcggaggc
    23101 cgtcactctt tattgagatc gcttaacaga acggcgatgc tttgacctcc cgctgtttgt
    23161 tcaagcgcaa ttttgacaat aattgttaac ggcacggaaa gcagcatacc caccggtcct
    23221 aacaaccatc cccaaaaaat caacgacaaa aataccacca atgtggaaag ccccagccca
    23281 cgtcccatga tgcgcggctc cagaatattg ccgaagacca gattaatcag cagatatccc
    23341 gccagcacca gcaacgcttc gtagaagcca ttaaacacca gtacctgagc gatagggggg
    23401 attgccgcga gaactgaacc aatattaggg atgtaattaa gcgcaaaggc cagcaatccc
    23461 cagacaaaag cgaagcgaac atcgagtgcg gcgagcatcg cccaggcgac caggccggtg
    23521 atgatgctga tggctgtttt cagcaccaga taatgagaaa cactgtcaat tgcacgttga
    23581 atcgccgcca tcccttcaac cggacgcgcc atcatttgct gaaattttcc gggcaattgt
    23641 ggcacttcga gcagcataaa cagcaccgtc agcagcaata aaaatattga taacatggca
    23701 ttagataact gcgtcaataa gttggtgagc aacgtcatcg ctgcgttcgg atcaatgtaa
    23761 tgcgccagct ggtcaactga gacgtcaatc cctacgcgtt gcaacaacgg ctcaagagct
    23821 tgcagcggcg tcataataga gttgcgatat tgcggtaacg tccgcgtcaa ctcgttgagc
    23881 gcggaaccca gataagctaa tagcaacacc atcgccatca cgatgatggt catcaaaatc
    23941 gacaccgcca gtacacgcgg cactcgccag cggaccatgt gttgcaccag cgggttaaga
    24001 ataacagcat tttatcattt agtgtttttt gctgttttat aatgtttcag gctgtgcatt
    24061 atgtttttga ttatcaacag atttgtgatt ctccttgttc cagcttgttt cacgttgtag
    24121 ccctttgtgc aacgatcggt gtagtcactg gtgtagtcac tttttcgtaa agagggggta
    24181 aaacgtgagc agagcaatga acaaactgag cgataccttg ctaaggaaac ttcatggtaa
    24241 gccagcggag aaaaacacgt tttatagtga tggcggaaac ctgagcgtga aatatttaac
    24301 atcagggaaa ttgacctggt atttcatata cagggccgga acgggaaggg agacacgacc
    24361 cgaacgcatt aagctgggaa gttatcctga tctgagcctg aaatcagcca gggaaaaagc
    24421 cgcccagtgt cgcgcatggc tggcagaggg gaaaaatcca cgtcatgagc ttaattacac
    24481 cgtacaggaa gcgttaaagc cggtaacggt tggcgatgcg ctcacctact ggcttgagta
    24541 ttacgtaaag gaaaaccgcg tggattatat cgccctgaaa aggcgactta ataatcacgt
    24601 aatacagcag attggtgcta tgccgctgga taaatgcgag ctacggcact ggctggcctg
    24661 ttttgaccag gtggcaaagc gaacgcctgt tactgccgga ttcgtgctac aggcgtgcaa
    24721 acaggcgctt aagttctgcc gtaggcggcg ctatgcaatc agcaacgttc tggacgatct
    24781 gaacgtggcg gacgttggga aaaaaccgga tataagcgaa cgtgtcttaa gtaacaaaga
    24841 acttggcgaa ttattgcagg cactggacaa aaaaatattc tccccttact acgtcgcgtt
    24901 aatccgcctc ctgattgtct tcggagccag aacggtagaa ctgaggttat cggagatcgg
    24961 ggagtgggat tttacggaaa tgctatggac cgttccgaag gagcacagca aaacgaaggt
    25021 cgcaatattc cggcccatac cggaagcgat cctgccgttc gtcacgcagc tggtggagca
    25081 gaacaggcac acgggcttat tgctggggga agtgaaacag gaggccagcg tatcgcagta
    25141 tggaagatta gcgcacagga ggcttaatca tcctcactgg tcactgcatg acatccggcg
    25201 cacctttaca accatgctga acgatttagg cgttgatccg catgtcgtgg agcagcttac
    25261 aggccaccag atgccaggaa tgcagcgagt ttataatcat tcccgttatc tggatgctaa
    25321 acgcaatgcg ctggatatgt ggacggagcg gttagggata ctggcgggaa cacatgaaaa
    25381 cgtaaccacg ctaccagtag ccagaagaaa ataatttttt tcgtgttttt tcagtatgcg
    25441 catactgtat atgcgaacag atacaacgtg caacaatgag caacaatgtg gaacaaccac
    25501 gaacaagagg cgaaaaagca tacagattta taagtggctg atttttaatg tgtcactggt
    25561 tttttataca ctagcgaatc actctttaaa gagcgagaaa aaaggcatga aacaatattt
    25621 tatgcctttg tattaacatg aatttaaaag attttattct tactgtttca ttttctgtaa
    25681 atcatacatc ctcaatattt gccattcatc actctagcgg gaacaatacg acacaataag
    25741 acacctgatg actctttaag aaacgaaagg gggggcaata gtgttaagca ctgatcggtt
    25801 tatacgtgaa aaagaatgcg aaaaactaac cggccttagc cgtacgtgtc gctaccgcct
    25861 ggaaaaggcc ggacaattcc catcacgtcg taaacttggc ggtcgttccg ttggctggtc
    25921 tttatccgag gttctggcct ggaaggatag ctgcaaggca gttcattaat cacgctggcg
    25981 gcacgcagcc gccacacatc aatcatctga acacagagct ataaccatga agattgaata
    26041 tacgccagaa cgtgggcggg gattcgttcg ccctggtgaa actgaaaaac aacaaaattg
    26101 gggtttttca ggtataaaaa aagcggcccc gaaatggagc cgcctttctg aacagataac
    26161 ccgctgcgcc gtttgtgtgt gtgatcccaa acataagcac ggggatgata gccgctatca
    26221 ggctggtggg caatgcaatc agtctggttc agttcgttgc catacctgca atgagcgctt
    26281 ttccctgtac tctttaagga attgctcaag ggcaaaagca catggcgcga atctttctga
    26341 ttcatgctct atctttctgc gccgtctttt ccgtgccggt gataatgttt tggtcaattc
    26401 tttatcggtc attgtgttgt cctgcatagc aatgcgccgt aataccttac accacggcgc
    26461 tgatggtgat tactctggtt ctttggcctt gcgccgctgg cgatattcaa cttctcgttt
    26521 taatgccgca gtaacaaatt gccccgtact ttcgccaggc atttttaccg cctcaacatt
    26581 gttcataact tcatgcggaa cccttgccgc aacggtttgt gattttgcgt ttactgcttt
    26641 tgtcgccatg tggtgtaccc ctcataaaaa acaaatgcag tatgcaggaa aaaaaataag
    26701 tgttcaacac ttgacgtgtt taacacctgg gtttaaattg gtgttcaaca ccttgttggc
    26761 gcaaggtgca gaaacgacga aacccggcag tgcgctaaca ctaaccgggc ttctaaccac
    26821 caacgatagc gaaagtatcg aggtagctat gagaaatcat accatacacc cgcaagggcg
    26881 ggactcgcac aacctgaata aatacatctg gcgttttatc gccctgagca cggcacaacc
    26941 gcgcgtgatt accattgagg ccaccagcga acaggaagca cgccagcaat ctccggctgg
    27001 ctgcgtgatg gtatttgctg cccgtattcg tcagggggta ggcttatgag ccaggaaatc
    27061 acactacaac aggcagcaga acgcgcccac caaatcgaag ttatttgcgc actggcagag
    27121 gattaccctg gcatgatgac cgacagcgaa tcaggggcaa tcatcggctt acttaaacgc
    27181 cttagcggtg aggtctgcgt attcctgagc gatgaacagg aaagaagaac gcttatttct
    27241 aacgaaaaaa aatgcggagg ggtacatgtg caataaaacc acaccggacg cagccgccgc
    27301 cgcgctcact acgctgatgc acgcgcttat tgatatttct gttattgctg acagggcgca
    27361 taaacacgca gccagagaat cagaatgcat cttccattat ttaacatttg tgcagctaaa
    27421 agccgatcag gcactggata aggccggaaa aattatcatg gctgatgtgc aggaggtgca
    27481 ccatgcataa cctgtcaatt tctgacctta acagcattca gtttgacgag aaatttaccg
    27541 ggcagttgct ggtcaatgtg gagaacgggc gcatagtgcg taattatcac ctgccggatg
    27601 gtgcaattgc cggaagcgtt gaagcattgc tggaactggc ggaacgtgcg cgactgatta
    27661 agccgtcaac gagccatcac gatgatgatc tgcattttac cggatgtatg gtgagtcact
    27721 acgaaaacgg cgtcgaggta tcctgcgaac ggctgcgtga tgattgctgt ttcggcacac
    27781 tgccggaatt tatcgagttg ttgaccagtt gcggttatca ggtcattcag ggggtaaaca
    27841 tgcgtgatga tcgttttaat tccctgaaac aggaattttc cggcgttcct gatgatgcgg
    27901 ctgatgcgct ttcgtcaatg ccagagctta ttagagcggc ttttttctta ctttccacga
    27961 gagaatataa atcaacgggg cttgatgtac tgaatatcgt cgccgattat gctgatttcg
    28021 tgacagaggt tattttaaga aaaacgacgg acggggatta atatgcgtga tatttacctt
    28081 gaaacaatag accgcgcatt tcttgcactt tctcacagtg aaaacatgct ggaaatatta
    28141 cgcatatggc ttgaaacact tggcgacaat gaacgcgaca aacaaaaatc aagaattgcc
    28201 acggcattaa taacgcttct tgagcctgta ataatggaac tgcaagaaat agatatattg
    28261 cacgacagat ataaagaaca gcacaccgga gaataaaaat aatgaaactt aaatattctg
    28321 gcttaactgc cagtggcaac actcacccta aatttacgcg cggcgatatt taccgcgacc
    28381 agtacggcgg caccgtaacg attaaaggtg tagaagagcg acgcattacc taccgccgtg
    28441 aaggctacga atatgactgc gtgatgcctg tttatcagtt ccggcgtgat ttttctctgg
    28501 tacaggcagc gccccgcagt aaaccaacca gcagggagaa agcacgcgcc aatattcagg
    28561 aaataaaaaa gatgcttaac gtattcaggg gcaagaaatg aaaagcgcac cgaacttaaa
    28621 aaaacagcct tacgacaaga tgaccgaagt catcattttt gcgggtagtg atgcgtgggc
    28681 acatgc
//
LOCUS       DAJVUJ010000035        28511 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.035, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000035 DAJVUJ010000000
VERSION     DAJVUJ010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28511
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.035"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(<1..84)
                     /locus_tag="OQQ48_001429"
     CDS             complement(<1..84)
                     /locus_tag="OQQ48_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCS1952253.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITAAIV"
     gene            complement(489..1589)
                     /gene="chaA"
                     /locus_tag="OQQ48_001430"
     CDS             complement(489..1589)
                     /gene="chaA"
                     /locus_tag="OQQ48_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HCS1952254.1"
                     /translation="MSNAQEAVKTRHKEISLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            1859..2089
                     /gene="chaB"
                     /locus_tag="OQQ48_001431"
     CDS             1859..2089
                     /gene="chaB"
                     /locus_tag="OQQ48_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HCS1952255.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKDDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            2247..2942
                     /gene="chaC"
                     /locus_tag="OQQ48_001432"
     CDS             2247..2942
                     /gene="chaC"
                     /locus_tag="OQQ48_001432"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HCS1952256.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGCTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(2986..3339)
                     /gene="ychN"
                     /locus_tag="OQQ48_001433"
     CDS             complement(2986..3339)
                     /gene="ychN"
                     /locus_tag="OQQ48_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HCS1952257.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            3523..4917
                     /gene="ychO"
                     /locus_tag="OQQ48_001434"
     CDS             3523..4917
                     /gene="ychO"
                     /locus_tag="OQQ48_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HCS1952258.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYFTWSQLGLTQQDNGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLLANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSLEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEYNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            complement(4918..5568)
                     /gene="narL"
                     /locus_tag="OQQ48_001435"
     CDS             complement(4918..5568)
                     /gene="narL"
                     /locus_tag="OQQ48_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HCS1952259.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(5561..7357)
                     /gene="narX"
                     /locus_tag="OQQ48_001436"
     CDS             complement(5561..7357)
                     /gene="narX"
                     /locus_tag="OQQ48_001436"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HCS1952260.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDEQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPASSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            7696..9087
                     /gene="narK"
                     /locus_tag="OQQ48_001437"
     CDS             7696..9087
                     /gene="narK"
                     /locus_tag="OQQ48_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HCS1952261.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            9603..13325
                     /locus_tag="OQQ48_001438"
     CDS             9603..13325
                     /locus_tag="OQQ48_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HCS1952262.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERAYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNQLMLTLGRGGPVVWLSEADAKELGIADNDWIEVYNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITGQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGND"
     gene            13343..14881
                     /gene="narH"
                     /locus_tag="OQQ48_001439"
     CDS             13343..14881
                     /gene="narH"
                     /locus_tag="OQQ48_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCS1952263.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            14878..15588
                     /gene="narJ"
                     /locus_tag="OQQ48_001440"
     CDS             14878..15588
                     /gene="narJ"
                     /locus_tag="OQQ48_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCS1952264.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEVIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            15588..16265
                     /gene="narI"
                     /locus_tag="OQQ48_001441"
     CDS             15588..16265
                     /gene="narI"
                     /locus_tag="OQQ48_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCS1952265.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFAGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(16448..16579)
                     /locus_tag="OQQ48_001442"
     ncRNA           complement(16448..16579)
                     /ncRNA_class="other"
                     /locus_tag="OQQ48_001442"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(16627..16711)
                     /locus_tag="OQQ48_001443"
     tRNA            complement(16627..16711)
                     /locus_tag="OQQ48_001443"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16675..16677),aa:Tyr,seq:gta)
     gene            complement(16746..16830)
                     /locus_tag="OQQ48_001444"
     tRNA            complement(16746..16830)
                     /locus_tag="OQQ48_001444"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16794..16796),aa:Tyr,seq:gta)
     gene            complement(16990..17832)
                     /gene="purU"
                     /locus_tag="OQQ48_001445"
     CDS             complement(16990..17832)
                     /gene="purU"
                     /locus_tag="OQQ48_001445"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HCS1952266.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVNHEGLSRNEHDQKMADAIDAYK
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            complement(17882..18340)
                     /locus_tag="OQQ48_001446"
     CDS             complement(17882..18340)
                     /locus_tag="OQQ48_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HCS1952267.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            18453..19358
                     /gene="rssA"
                     /locus_tag="OQQ48_001447"
     CDS             18453..19358
                     /gene="rssA"
                     /locus_tag="OQQ48_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HCS1952268.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQCREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGESLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            19450..20463
                     /gene="rssB"
                     /locus_tag="OQQ48_001448"
     CDS             19450..20463
                     /gene="rssB"
                     /locus_tag="OQQ48_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HCS1952269.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDATVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            20665..21573
                     /gene="galU"
                     /locus_tag="OQQ48_001449"
     CDS             20665..21573
                     /gene="galU"
                     /locus_tag="OQQ48_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCS1952270.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEQVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(21717..22130)
                     /gene="hns"
                     /locus_tag="OQQ48_001450"
     CDS             complement(21717..22130)
                     /gene="hns"
                     /locus_tag="OQQ48_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HCS1952271.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKARRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            22735..23352
                     /gene="tdk"
                     /locus_tag="OQQ48_001451"
     CDS             22735..23352
                     /gene="tdk"
                     /locus_tag="OQQ48_001451"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCS1952272.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            complement(23634..24530)
                     /locus_tag="OQQ48_001452"
                     /pseudo
     CDS             complement(23634..24530)
                     /locus_tag="OQQ48_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(24654..27329)
                     /gene="adhE"
                     /locus_tag="OQQ48_001453"
                     /gene_synonym="adhC"
     CDS             complement(24654..27329)
                     /gene="adhE"
                     /locus_tag="OQQ48_001453"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCS1952273.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGILSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            27807..28454
                     /gene="ychE"
                     /locus_tag="OQQ48_001454"
     CDS             27807..28454
                     /gene="ychE"
                     /locus_tag="OQQ48_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HCS1952274.1"
                     /translation="MIQTFFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
BASE COUNT         6992 a         6861 c         7433 g         7225 t
ORIGIN      
        1 gacaatcgct gcggtaataa tccccgccag gatcggtgct gccaggtcgt gccagaaagt
       61 catggcaaac tgcgcgagcg tcatatagcc gccttgttgt aatgacaaca ttttgcggct
      121 attcttgaag tgtctggttt caagattagc ccccgttttg ttgtcaggtt ttacctctca
      181 acgtgcgggg gttttctctt tccagcaacc aatgccacca gggataaagc ccccgcaaca
      241 ttgcgcctca ccggataatt ccggcttggt gtggatacta cgtctcaatt catcttcact
      301 tcatccctga aatgtttgca ataaagagta cattccggct tttcaacagc tgttgcagtg
      361 gtttcgtgca ggttgtggta caggctcgca attctgatta acgagctgtc caggtagtat
      421 gaagaaatac aataagcagg aatttatctt atctcccccg atttccgtca tcggaggaga
      481 tataactgtc aggcaaatat cgtcatcaaa taggcggcaa acagtgccag atgcgctgcg
      541 ccattgagca cgttagtacg tccggtggag aaggagatat ggcacagcac taaagaggcc
      601 accatcacca ccatttctgg cgcaccaagt gcaaactgta attcgttacc cgtcataaag
      661 gcaattagcg tgacgacagg tacggtaagt gaaatggttg ctaacactga accaaagaac
      721 agattcatcg cgcgctggac ctggttgttc aacactgctt ttaacgcacc taaaccttcc
      781 ggcgacagaa tcaacagagc caccaggaag ccagtaaagg cgacaggggc attcatgctg
      841 tcgagcaatg tctccagcgg gctggcattc attttggtga ctgcaataac ggcaatcaga
      901 tggataatca accagatagc atgccacacg ctgctatggg cagacggttt accgtgatgc
      961 gggtcgtcgt catcactgtc atcttcgtgc tcgtagacaa acaaactttg atgcgttttg
     1021 gtctggatca gcaaaaatac gccatacatt gccgcagaaa ttaatgctac cagtaacgcc
     1081 tgaccggttg aaaaattcgc cgcaggcaga gccattggaa ataccagtac gattatcgcc
     1141 agggggaaca gcgcaattaa atactgcttg ataccaaaca gattcatata ttgggtggca
     1201 aacttacgac cgcccaacaa taatgaaaag ccaaccagcc caccggtaac aatcataatg
     1261 attgaataga gcgtatcacg cattagcgtt ggcgcggcgt cgccggttgc cattaaagct
     1321 gaaatcaaac tgacttcaag aataaccact gaaaggctaa gaataagcga accgtaaggt
     1381 tctcccaagc gatgggctaa tacgtccgca tgacggacaa cactaaaggc gctacttaaa
     1441 ataccaataa gcgcaagaag attgatggca atgaccactg gtagtgtctg gctgcttccc
     1501 cacaggaaca gcactaccag cgccagaacc gggaaaataa gcgaaatctc cttgtggcgg
     1561 gtttttaccg cctcttgagc atttgacatt atggttatcc ctttgcagat gaatttatcg
     1621 aaaatgtaaa aaataggtag gaaaaataac agaaagtgtc tggatatcgg taacatttta
     1681 cgaattttta ccccactgtc gattttttac tcattgggac ataaaaataa gtattacgtt
     1741 tagacaatgt ttgttttagc gtttcttaag aacagtctga catgaaaatt cttatgtttt
     1801 ggcaagagta gatattgttg accacactta atgttcaact ttgtaaaagg agtcaacgat
     1861 gccgtataaa acgaaaagcg atctgccgga aagcgtaaag cacgttctac cgtctcatgc
     1921 ccaggatatc tataaagaag cgttcaacag cgcatgggat caatataaag ataaagacga
     1981 tcggcgtgat gacgccagtc gcgaagaaac cgcgcataaa gtggcctggg ctgctgtgaa
     2041 gcatgaatat gccaaagggg atgatgataa atggcataaa aaatcgtaaa accggtagct
     2101 tagttaaagc tattcgtgcg gtgttgcctt gcaagtggtc cgtggattgc atattgtccc
     2161 gttagtggtt tcaaaatgag cagtaaaaat gtccggaaga caccaaaaag ttgtcgcagg
     2221 gaagtatgca gtggcggagg tgtaaggtga taacgcgtga tttcttgatg aatgccgatt
     2281 gtaaaacggc atttggtgcc attgaagaat cactcttatg gtcagcagag caacgagcgg
     2341 cttcgctggc ggcgacgctg gcttgtcgac ctgatgaggg accggtgtgg atcttcggtt
     2401 atggatcgtt gatgtggaat ccggcactgg agtttaccga gtcgtgcacc ggtacactgg
     2461 ttggatggca tcgcgcgttt tgcctgcgcc tgaccgccgg gcgcgggact gcgcaccagc
     2521 cgggacggat gcttgcactg aaagagggcg gacgcactac aggcgtcgcc tatcgactgc
     2581 cagaagagac gctggagcag gaactaaccc tgttgtggaa gcgcgagatg attaccggct
     2641 gttatctgcc aacctggtgt cagcttgatc ttgatgatgg atgcacagta aacgccattg
     2701 tgtttattat ggacccgcga catccagaat atgaatctga cactcgcgct caggtcatcg
     2761 cgccgttgat tgcggcggcg agcggtccgc tgggaaccaa cgcacaatac ctgttttcac
     2821 tggaacagga gcttatcaaa ctgggaatgc aggacgatgg gctgaatgat ttgctggtat
     2881 cggtaaaaaa actgctggcg gagaattatc cggatggtgt gttacggccg ggattcgcct
     2941 gagtaaactt cccgcatagt ggggcgtcag acgccccctc aaacattaaa atgtgagcac
     3001 tttatcggct gacagcgtcc attgcgccag ttccacaaga gtaccgattt ccaccccatc
     3061 aatcagagga agtgtactaa tcccgcgccc gtcggtacag gttttgcaca atttcaccgg
     3121 tacattctga gcggtaagga tctccagcat ttgctgaatg ttgtagcctt cccctggttt
     3181 ttgcccgcgc aacccggctg tgaccgcatc agacatgagg aacagacgca gatccagatt
     3241 gctctcctgc tctcgtaacg caatggccag ccgcaagctg ttaaacaagg attcgctccc
     3301 gtaaggtgcg ccattggcaa cgatcacgat tttttgcatt atttactcct gtattcaggg
     3361 aattagacac tcatcttcta tcttactgct tctgcagcgt ctgaccaatc ggtcacattt
     3421 ttaaggattt tcctgaaagc gcgagaaaat acgacaaaag ttgccagtaa tcgttattct
     3481 ttaaggctat ggttttcatt attaccggaa gttaccgacg ttttgagccg tttcgttcct
     3541 cgcattatcc cgttttattt actcttgctt gtagcaggcg gtacagctaa cgcacaatct
     3601 accttcgagc aaaaagcggc aaatcccttt gataataaca atgatggtct gccggattta
     3661 ggcatggctc ccgaaaatca tgatggggaa aaacactttg ctgaaattgt gaaagatttc
     3721 ggcgaaacca gtatgaatga taacgggctg gatactggcg agcaggcaaa agctttcgca
     3781 ttgggaaaag tccgcgacgc gcttagtcaa caggttaatc agcacgtaga gtcctggcta
     3841 tcaccgtggg gaaatgccag tgttgacgtc aaagtggata acgaaggaca tttcaccggc
     3901 agtcgtggaa gctggtttgt gccgttacaa gataatgatc gttatttcac ctggagccag
     3961 cttggtctta ctcagcagga taatgggttg gtgagcaatg tgggcgttgg gcaacgctgg
     4021 gcgcgcggca actggctggt gggttataac actttttatg acaacttgct ggacgaaaat
     4081 cttcagcgag cgggctttgg tgccgaagcg tggggcgaat atttgcgatt attggcaaac
     4141 ttttatcagc cgtttgctgc atggcatgaa cagacagcca cgcaggaaca acggatggcg
     4201 cgcgggtacg acctgacagc tcggatgcgc atgccgttct atcaacacct caataccagt
     4261 gtcagcctag aacagtattt tggtgatcgt gttgatttgt ttaactctgg tacgggttat
     4321 cacaatcccg tcgcgttgag tctgggatta aattacaccc ctgtgccatt agtcactgtg
     4381 acggcccagc ataaacaggg tgaaagtggc gagaatcaaa ataacctcgg gctgaatctt
     4441 aattaccgct ttggtgtacc gctcaaaaaa caactttctg cgggcgaggt tgccgaaagt
     4501 cagtcgttac gtggtagtcg ctatgacaat ccgcagcgaa ataatctacc gactcttgag
     4561 taccgacagc gaaaaacgtt aacggtgttt ctggcgacac cgccgtggga tctaaaacct
     4621 ggcgaaacag tgccgctgaa attacaaatc cgcagtcgtt acggtattcg gcaactgatt
     4681 tggcagggcg atacgcagat attaagtttg acgccgggcg cacaagccaa cagcgcggag
     4741 ggctggacgc tgatcatgcc tgactggcag aacggggaag gggcaagcaa tcactggcga
     4801 ttgtcggtgg tggtggaata taaccagggg cagcgtgtct cctccaatga gatcacgcta
     4861 acgcttgtcg aaccgttcga tgcattgtca aacgacgaac tgcgctggga accgtaatca
     4921 gaaaatgcgc tcctgatgca cccataccgc tgcttccacg cgagacttga gcttcatttt
     4981 cttcagcatg tgcttgacgt gcacttttac tgtgctttcg gtgatatcca ggcggcgggc
     5041 aatcatcttg ttcggcaaac cctgggcaat cagcttgaga atatcgcgct cgcgtggggt
     5101 taactggtta acatcgcgct cagtagtggc acggttagcg cgcaagctgg cggccagaac
     5161 aggcgttaat gcttcgctta ataccatttc gccagcagct gcctgatgca atgctttcag
     5221 cagatcttcc ggttccatat cttttaacag ataaccatcc gcgccgcgtt tcagtgcggt
     5281 gaccacatct tcttcatggt tagagacgct gaataccaca atgcgccctg agagggactt
     5341 ttcgcgcagt ttatccagcg tttccagacc gttcatgccg ggcatattga gatctaacag
     5401 gatcagatcg ggatcaagag actccgccag ttcaataccc tgttcgccat tactcgcttc
     5461 gccaaccacg gtgatatctg gtgccatact gataagctgt tttacgccag ttcgcagcat
     5521 cgggtgatcg tcgatcagca ggatagtagc cggttcctga ttactcatgg gtatctcctt
     5581 ggacgtctgt gaaagttttt tcgggaataa aggtgaccac cacttcggtg ccacctgatt
     5641 cacgacggcg gacgcggcaa tcgcctcgta aactttgcgc acggtcgcgc attattatca
     5701 tgccgtagtg attgctgcgg atggcatttt caggcacgcc gcagccgtta tcttggacgg
     5761 tcagtttgac ctgattatcg ttttgcgcca ccgtcaccac gacctcactc gcttgcgaat
     5821 gtttgagggc gttacttaat gcctcacggg caatttgcaa caagtggatt gcctgatgtg
     5881 aaggcaccag acgaggcggc aattgataat ccagcttcac cgggaagcca aatttggcgc
     5941 tgtactcttc gcaactcgcc tccagcgccg gacgtaatcc aggctcggtg agctgcaagc
     6001 ggaatgtggt gagcaattca cgcaactgcg cccaggatgc attcagttcg ttacggatct
     6061 gacttaacag ttcgcggctg cttgctggca gcgcatcgcc ctgcatctgt aaacaactca
     6121 cctgcatctt catgcaagag agagattggg caatagaatc atgcagttcg cgcgcaatgg
     6181 tggcacgctc ttccatcacg atcaactgtt gctgacgttc ctgatggcga tccagcgcca
     6241 gcgtggcggt gagttgttca accagggtat ccaccagttg ttgttgatca tggctaagat
     6301 gacgcccctg cggcagggtc gccagcaaaa taccgtactg cgtatgagag tcagccagcc
     6361 gccacttcag ggttgtgccg cgatcaccaa cgggtaatac gccgcgcggg cagagctggc
     6421 agcctttatc atcacaagtc atatctggct ggcaggtaaa ctcctgatga ttctcttcat
     6481 catcagtgtc atacacccgc agttcgatat cacgtagcag ggttaaattc tgtaagccgt
     6541 tgagtacagg tgacaggcgt tcacacagcg gggcgcggga atgcaaacgg cggttagcct
     6601 gccataaaaa agagaggatc tgatttttat gctccagccc ggcggttttc tcctgaaccc
     6661 gctgctcaag tacggcataa ctttcggcca gttctgcaga catattgttc aatgcagttc
     6721 caagcatcgc catttcgttg cgcccgctga tgttcgcgcg ttgggtaaaa tcgcgatgac
     6781 tgacggcact cgccattgcc agcagttgcc gccacggttg tagcagtcgc gcccgcaacc
     6841 agataatagt gaacaccagt aaaagtgcca taaataccgc cattacccga tggaccagta
     6901 ccactgtctc aatgcgcatt tccgtggtgc ggtcaaaacc agataccagc tgatcaagcc
     6961 cggcaacaaa ctggctgaca tccgctgaca ccgtttcgcg gttttgtgca cgcatcagtg
     7021 cagggatcag ttcattacgc cagtaatctt gtaaaccctg taattgcgcc agttgttcgt
     7081 ctcgttctgc tgctcgagtt aactcggcgc taaatgccgt ttgttccatc tctttaatta
     7141 agggcttgtc tttctcactt aatggcactg ccgccaacag acggtaactt tgcatgcgca
     7201 gcgatcccgc tttgttgatc gcatgggcgc tgccctgaac gccttgcacc agccagccag
     7261 aaaccgccat ccctgccagt ccaatagcag tagaaagcaa cacaataagc gcaacctgat
     7321 taaccagggt gagcggagag agacaacgtt taagcatgta aacctcttcc ttcaggcttt
     7381 aaatgagcaa taaccttaat gaatgtgacg atacattctg gaatggcagt attctcggct
     7441 attggctgaa gtatacccat acccggaaag agttactcct tatttgccgt gtggttagtc
     7501 gctttacatc ggtaagggta gggattttac agcaccgtga aaaatctcat aatttttatg
     7561 aaatcactgt actcactatg ggtattgata aatatcaatg atagataaag ttatcttatc
     7621 gtttgattta catcaaattg cctttagcta cagacactaa ggtggcagac atcgaaacga
     7681 gtatcagagg tgtctatgag tcactcatcc gcccccgaaa gggctactgg agctgtcatt
     7741 acagattggc gaccggaaga tcctgcgttc tggcaacaac gcggtcaacg tattgccagc
     7801 cgtaacctgt ggatttccgt tccctgtctg ctgctggcgt tttgcgtatg gatgttgttc
     7861 agcgctgttg cggtgaactt accgaaagtc ggttttaatt ttacgaccga tcagctattt
     7921 atgttgactg cgctgccttc ggtttctggc gcgttattac gtgttccata ctcctttatg
     7981 gttcctatct tcggtggtcg tcgctggacg gcgttcagca ccggtattct gattattcct
     8041 tgcgtctggc tgggttttgc cgtgcaggat acctccacgc cttatagcgt cttcatcatc
     8101 atctctctgc tatgcggctt tgctggcgcg aacttcgcat ccagtatggc aaacatcagc
     8161 ttcttctttc cgaaacagaa gcagggtggc gcgctgggtc tgaatggtgg tctgggaaac
     8221 atgggcgtca gcgtcatgca gttggttgct ccgctggtgg tatcactgtc gattttcgca
     8281 gtatttggta gccagggcgt caaacagccg gatgggactg agctgtatct ggcgaatgcg
     8341 tcctggatat gggtgccgtt ccttgccatc ttcaccattg cggcgtggtt tggcatgaac
     8401 gatcttgcta cctcgaaagc ctccatcaag gagcagttgc cggtactcaa acggggtcat
     8461 ctgtggatta tgagcctgct gtatctggca accttcggct ccttcatcgg cttctccgcg
     8521 ggctttgcaa tgctgtcaaa aacgcagttc ccggatgttc agattctgca atacgctttc
     8581 ttcgggccgt ttattggtgc gctggcgcgt tctgcaggtg gtgcattatc tgaccgtctg
     8641 ggcggaactc gtgtcacgct ggtgaacttt attctgatgg cgattttcag cggcctgctg
     8701 ttcctgacct taccgactga cgggcagggc ggaagcttca tggcgttctt cgcggtcttc
     8761 ctggcgctgt tcctgacagc tgggctgggt agtggttcca ctttccagat gatttcagtg
     8821 atcttccgta aactgacaat ggatcgcgtg aaagcagaag ggggttctga cgaacgtgcg
     8881 atgcgtgaag cggcaaccga cacggcggcg gcgctgggtt tcatctctgc gattggcgcg
     8941 attggtggct tctttatccc gaaagcgttt ggtagctcgc tggcattaac gggttcgcca
     9001 gtcggcgcaa tgaaggtatt tttgattttc tatatcgcct gcgtagtgat tacctgggcg
     9061 gtatatggtc ggcattctaa aaaataaacc gttactcgtc atactccggg ttacatgtgc
     9121 tgcggctgcg ttcattcacc ccagtcactt actttagtaa gctcctggga ttcattcact
     9181 tgccgccttc ctgtaaaccg aattatatag agtaaaatat ttgattatcc tttgcgcggc
     9241 atgatgtcgc gcttttttta agcgttattt agttacaaca tactaatgtt atatggttta
     9301 tttcgccgga tttcattaag agccattaat atgttcccca tggggaatac tccttaatac
     9361 ccatctgcat aaaaatctta atagtttaaa taactacagg tataaaacgt cttaatttac
     9421 agtctgttat gtggtggctg ttgattatcc taaaggggta tcttaggaat ttactttatt
     9481 tttcatcccc atcactcttg atcgttatca attcccacgc tgtttcagag cgttaccttg
     9541 cccttaaaca ttagcaatgt cgatttatca gagggccgac aggctcccac aggagaaaac
     9601 cgatgagtaa attcctggac cggtttcgct acttcaaaca gaagggtgaa acctttgccg
     9661 atgggcatgg ccagcttctc aataccaacc gtgactggga ggatggatat cgccagcgtt
     9721 ggcagcatga caaaatcgtc cgctctaccc acggggtaaa ctgcaccggc tcctgcagct
     9781 ggaaaatcta cgtcaaaaac ggtctggtca cctgggaaac ccagcagact gactatccgc
     9841 gtacccgtcc ggatctgcca aaccatgaac ctcgcggctg cccgcgcggt gccagctact
     9901 cctggtatct ttacagtgcc aaccgcctga aatacccgat gatgcgcaaa cgcctgatga
     9961 aaatgtggcg tgaagcgaag gcgctgcata gcgatccggt tgaggcatgg gcttctatca
    10021 ttgaagacgc cgataaagcg aaaagcttta agcaggcgcg tggacgcggt ggatttgttc
    10081 gttcttcctg gcaggaggtg aacgaactga tcgccgcatc taacgtttac accatcaaaa
    10141 actacggccc ggaccgtgtt gctggtttct cgccaattcc ggcaatgtcg atggtttctt
    10201 acgcatcggg tgcacgctat ctctcgctga ttggcggtac ttgcttaagc ttctacgact
    10261 ggtactgcga cctgcctcct gcgtctccgc aaacctgggg cgagcaaact gacgtaccgg
    10321 aatctgctga ctggtataac tccagctaca tcatcgcctg gggttcaaac gtgccgcaga
    10381 cgcgtacccc ggatgctcac ttctttactg aagtgcgtta caaaggcacc aaaactgttg
    10441 ccgtcacacc agactacgct gaaatcgcca aactgtgcga tctgtggctg gcaccgaaac
    10501 agggcaccga tgcggcaatg gcgctggcga tgggccacgt aatgctgcgt gaattccacc
    10561 tcgacaaccc aagccagtat ttcaccgact atgtgcgtcg ctacaccgac atgccgatgc
    10621 tggtgatgct ggaagaacgc gacggttact acgctgcagg tcgtatgctg cgcgctgctg
    10681 atctggttga tgcgctgggc caggaaaaca atccggaatg gaaaactgtc gcctttaata
    10741 ccaatggcga aatggttgcg ccgaacggtt ctattggctt ccgctggggc gagaagggca
    10801 aatggaatct tgagcagcgc gacggcaaaa ctggcgaaga aaccgagctg caactgagcc
    10861 tgctgggtag ccaagatgag atcgctgagg taggcttccc gtactttggt ggcgacggca
    10921 cggaacactt caacaaagtg gaactggaaa acgtgctgct gcacaaactg ccggtgaaac
    10981 gcctgcaact ggctgatggc agcaccgccc tggtgaccac cgtttatgat ctgacgctgg
    11041 caaactacgg tctggaacgt ggcctgaacg acgttaactg tgcaaccagc tatgacgatg
    11101 tgaaagctta taccccggcc tgggccgagc agattaccgg cgtttctcgc agccagatta
    11161 ttcgcatcgc ccgtgaattt gccgataacg ctgataaaac gcacggtcgt tcgatgatta
    11221 tcgtcggtgc ggggctgaac cactggtatc acctcgatat gaactatcgt ggtctgatca
    11281 acatgctgat tttctgcggc tgtgtcggtc agagcggggg cggctgggcg cactatgtag
    11341 gtcaggaaaa actgcgtccg caaaccggct ggcagccgct ggcgtttgcc cttgactggc
    11401 agcgtccggc gcgtcacatg aacagcactt cttatttcta taaccactcc agccagtggc
    11461 gttatgaaac cgtcacggcg gaagagttgc tgtcaccgat ggcggacaaa tcccgctata
    11521 ccggacactt gatcgacttt aacgtccgtg cggaacgcat gggctggctg ccgtctgcac
    11581 cgcagttagg cactaacccg ctgactatcg ctggcgaagc ggaaaaagcc gggatgaatc
    11641 cggtggacta tacggtgaaa tccctgaaag agggttccat ccgttttgcg gcagaacaac
    11701 cagaaaacgg taaaaaccac ccgcgcaacc tgttcatctg gcgttctaac ctgctcggtt
    11761 cttccggtaa aggtcatgag tttatgctca agtacctgct ggggacggag cacggtatcc
    11821 agggtaaaga tctggggcaa cagggcggcg tgaagccgga agaagtggac tggcaggaca
    11881 atggtctgga aggcaagctg gatctggtgg ttacgctgga cttccgtctg tcgagcacct
    11941 gtctctattc cgacatcatt ttgccgacgg cgacctggta cgaaaaagac gacatgaata
    12001 cttcggatat gcatccgttt attcacccgc tgtctgcggc ggtcgatccg gcctgggaag
    12061 cgaaaagcga ctgggaaatc tacaaagcca tcgcgaagaa attctccgaa gtgtgcgtcg
    12121 gccatctggg taaagaaacc gacatcgtca cgctgcctat ccagcatgac tctgccgctg
    12181 aactggcgca gccgctggat gtgaaagact ggaaaaaagg cgagtgcgac ctgatcccag
    12241 gtaaaaccgc gccacacatt atggtcgtag agcgcgctta tccggcgact tacgaacgct
    12301 ttacctctat cggcccgctg atggagaaaa tcggtaatgg cggtaaaggg attgcctgga
    12361 acacccagag cgagatggat ctgctgcgta agctcaacta caccaaagcg gaaggtccgg
    12421 cgaaaggcca gccgatgctg aacaccgcaa ttgatgcggc agagatgatc ctgacactgg
    12481 caccggaaac caacggtcag gtagccgtga aagcctgggc tgccctgagc gaatttaccg
    12541 gtcgtgacca tacgcatctg gcgctgaata aagaagacga gaagatccgc ttccgcgata
    12601 ttcaggcaca gccgcgcaaa attatctcca gcccgacctg gtctggtctg gaagatgaac
    12661 acgtttctta caacgccggt tacaccaacg ttcacgagct gatcccatgg cgtacgctct
    12721 ctggtcgtca gcaactgtat caggatcacc agtggatgcg tgatttcggt gaaagcctgc
    12781 tggtttatcg tccgccgatc gacacccgtt cggtgaaaga agtgataggc cagaaatcca
    12841 acggcaaccc ggaaaaagcg ctcaacttcc tgacgccgca ccagaagtgg gggatccact
    12901 ccacctacag cgacaaccag ttgatgctga ccttaggtcg cggtggcccg gtggtctggc
    12961 tgagcgaagc cgatgccaaa gagctgggca ttgccgataa cgactggatt gaagtttaca
    13021 acagcaacgg tgcgctgact gcccgtgcgg ttgtcagcca gcgtgttccg gcagggatga
    13081 ccatgatgta ccacgcgcag gaacgtatcg ttaacctgcc gggttcggaa attactggcc
    13141 agcgtggggg gatccacaac tcggtcaccc gtatcacgcc gaaaccgacg catatgattg
    13201 gtggctatgc ccatctggca tacggcttta actactacgg caccgtaggt tctaaccgcg
    13261 atgagtttgt tgtagtgcgt aagatgaaga acattgactg gttagatggc gaaggcaatg
    13321 actaggtaca ggagagcgta aaatgaaaat tcgttcacaa gtcggcatgg tgctgaatct
    13381 cgataagtgc atcggctgcc acacctgttc agttacctgt aaaaacgtct ggaccagccg
    13441 tgaaggcgtg gagtacgcgt ggttcaacaa cgtggaaacc aagccgggtc agggcttccc
    13501 gactgactgg gaaaaccagg aaaaatacaa aggcggctgg atccgtaaaa tcaacggcaa
    13561 actgcagccg cgcatgggta accgtgccat gttgctgggt aaaatcttcg ctaacccgca
    13621 tctgccgggg atcgacgatt attacgagcc gttcgatttt gactatcaga acctgcatac
    13681 cgcgccggaa ggcagcaaat cgcagccgat tgcccgtccc cgttcgctga ttaccgggga
    13741 acggatggcg aaaatcgaaa aagggccgaa ctgggaagat gacctgggtg gtgagtttga
    13801 caaactggcg aaagacaaga acttcgacaa catccagaag gcgatgtata gccagttcga
    13861 aaacaccttt atgatgtatt tgccgcgcct gtgcgaacac tgcctgaacc cggcatgtgt
    13921 ggcgacctgc ccgagcggtg cgatttacaa gcgtgaagaa gatggcatcg tcctgatcga
    13981 ccaggataaa tgccgtggct ggcgtatgtg catcaccgga tgcccgtaca aaaaaatcta
    14041 cttcaactgg aagagcggta agtctgagaa gtgcatcttc tgctatccgc gtattgaagc
    14101 gggtcagccg accgtgtgct cagaaacctg tgtcggtcgt atccgttatc ttggcgtgct
    14161 gctgtacgat gccgacgcga ttgaacgtgc agccagcacc gagaacgaga aagatcttta
    14221 ccagcgtcag ctggaggtgt tcctcgatcc gaacgatccg aaagtcatcg agcaggcgat
    14281 taaagacggt attccgctga gcgttattga agccgcacag cagtcgccgg tttataaaat
    14341 ggcaatggaa tggaaactgg cgctgccgct gcatccagaa tatcgcacac tgccgatggt
    14401 ctggtacgtg ccgcctctgt ctccgattca gtctgcagca gacgcgggtg agctgggtag
    14461 caacggcatt ctgccagacg tcgaaagctt gcgtattccg gtacagtatc tggcgaatct
    14521 gctgaccgcc ggtgatacca aaccggtact gcgcgcactg aaacgtatgc tggcgatgcg
    14581 tcattacaaa cgtgctgaaa ccgttgacgg taaagttgat acccgtgcgc tggaagaggt
    14641 cggtctgacc gaagcccagg cacaggagat gtaccgttat ctggctattg ctaactacga
    14701 agatcgcttt gtggtgccga gtagtcatcg tgaactggca cgggaagcct tcccggagaa
    14761 aaatggctgc ggctttacct ttggtgatgg ctgccacggc tcagatacca aattcaatct
    14821 gttcaacagc cgtcgtatcg atgccatcga tgtgaccagc aaaacggagc cgcatccatg
    14881 atcgaactcg tgattgtatc gcgtctcctt gaatatccgg atgctgcctt atggcagcat
    14941 caacaagaga tgtttgaggt gattgctgcg tcgaaaaatc tgtcgaaaga agatgcccat
    15001 gcgctgggca ttttcctgcg cgatttaacg gcgatggatc cgctcgatgc ccaggcccag
    15061 tacagcgaac tgttcgaccg tggccgcgcc acgtcactgt tgctatttga acatgttcac
    15121 ggtgaatccc gtgaccgcgg ccaggcgatg gtggacctgc tggcgcagta cgagcagcac
    15181 ggcttgcagt taaacagccg cgaattaccg gaccatctgc cgctgtatct ggagtacctg
    15241 tcgcagctgc cgcaaagcga agccgtggaa ggtttgaaag atatcgcgcc gattctggca
    15301 ttgctgagcg cgcgtctgca acagcgtgaa agccgttatg ccgtgatgtt tgatctgctg
    15361 ctgaaattgg ccaataccgc tatcgacagc gacaaagtgg cggaaaaaat tgccgacgaa
    15421 gcgcgcgatg atacgccgca ggcgctggat gctgtctggg aagaagagca ggttaaattc
    15481 tttgctgaca aaggctgcgg tgattcagca atcactgctc atcagcgtcg ctttgccggt
    15541 gccgtcgcgc cgcaatatct gaatatcacc accggaggac agcactaatg caattcctga
    15601 atatgttctt ctttgatatc tacccgtaca tagccggggc ggtcttcctg attggtagct
    15661 ggctgcgtta tgactacggg cagtacacct ggcgcgcggc gtccagccag atgctggatc
    15721 gcaaagggat gaacctggcg tcgaacctgt tccatatcgg tattctgggg atttttgctg
    15781 gtcacttctt cggtatgctg acgccgcact ggatgtatga agcctggctg ccgattgaag
    15841 tgaaacagaa aatggcgatg tttgctggtg gtgccagcgg cgtgctgtgt ctgattggcg
    15901 gcgtgctgtt gctgaagcgt cgtctgttca gcccacgcgt gcgtgcaacc actaccggag
    15961 cggatatcct gatcctgtcg ctgctcgtta tccagtgcgc gctgggcctg ttgaccattc
    16021 cgttctccgc tcagcatatg gacggtagcg agatgatgaa actggttggc tgggcgcagt
    16081 cggtggtgac cttccacggt ggcgcttctc aacacctcga tggtgtggcg tttatcttcc
    16141 gtctgcacct ggtgctgggg atgacgttat tcctgctgtt cccgttctcg cgtctggtac
    16201 acatctggag cgtaccggtg gagtatctga cacgtaagta ccagctggtg cgcgctcgtc
    16261 actaaacgaa ttttagttca catagaccct gcttcggcgg ggttttttat gggcacggtg
    16321 cggggtgagt tgtcggatgc gcttcgctta tccgacctac aggggaggat attgtaggcc
    16381 cggtaaacgt ggtgccatcg ggcaaaatta gctcggagta acaggttttg attattcaag
    16441 gaagcgatgg tggtggggga aggattactc agcgctgcgc gcttcgccct tcgggtcgtt
    16501 gcctgcggca acgctctctc gctggcgctc aagtcgaacc ttggtcgaag cttctcatcc
    16561 ttccccgctt gggcagaata tttaattgcg gattcgtttg agagtttacg gacttttgaa
    16621 aagtgatggt ggtgggggaa ggattcgaac cttcgaagtc gatgacggca gatttacagt
    16681 ctgctccctt tggccgctcg ggaaccccac caggggtaat tcaaattttg aggtaatgct
    16741 tgagatggtg gtgggggaag gattcgaacc ttcgaagtcg atgacggcag atttacagtc
    16801 tgctcccttt ggccgctcgg gaaccccacc acggggtaat gctttttact ggcctgctcc
    16861 cttatcggga agcggggcgc atcatatcaa atgacgcgcc gctgtaaagt gttacgttga
    16921 gaaaaatgaa ctggttgcgt aattttcatc cgtaacggat taaaggtaac cagttatttt
    16981 tgctggcgat taaagaataa tcgtccgatt accgtaaaca aagacgcgct gtgccagtac
    17041 tttgtatagc gcacgactta aaacgttttt ctcgacgtca cgacctgcgc gcatcatatc
    17101 ttcagctgtg taggtatgat cgacatgaat aacgtcctgc atgatgattg ggccttcgtc
    17161 cagattgtca ttcacatagt gagcggttgc gccaataatc ttcacaccac gttcataggc
    17221 ctggtgataa ggacgtgcgc cgataaacgc aggcaggaat gaatggtgaa tattgatgat
    17281 tttattcggg aagcgtgcca caaattccgg cgttaatacc cgcatatact tcgccagcac
    17341 cacatagtca ggtttataag catcaatggc gtctgccatc ttttgatcgt gctcgttgcg
    17401 gcttaaccct tcatggttta ccagctcaaa cggaatatca aaacgctcaa ccagagaacg
    17461 taaagtatcg tggttaccaa taactgccgc gatttcgaca tccaggccgc cgtaattggc
    17521 tttcatcaac aaatcgccaa ggcaatgcgc ttctttagtg accagaatca ctatccggcg
    17581 acgaccggca ggattcagct cacgcacgga gccttctggc aatgcgctat cgagatccgc
    17641 cagcagggtg gaatcattaa aaatcccttc cagttccgtg cgcataaaaa agcgcccggt
    17701 acggtgatca acaaattcat tgttctgtac gatatttaac tcgtgcttgt agcaaatatt
    17761 ggtaatacgt gcgatcagac ctttttggtc cggacaaata gtacgcagaa ctttacgttg
    17821 gagtgaatgc attgctggaa aaaccttgtt gagagtgttt gctaaaccgt accgtcaacc
    17881 attattggcc gcagcacttt ttaaattttt tacctgaacc acaagggcag ggatcgttgc
    17941 gaccaaactg cggacgtgta ccgtcaatat agtaccattg accgttttct ttcaaaaatc
    18001 gcgaacgttc aattattgca cctgttttgc caccttcggt aaatcgagca acaaaactga
    18061 caaatccgat attgtcggcg tcctgccaac aatgttcaaa tacggtcaat cccaaccatt
    18121 cagtatgcgc aaatccggcc atcaattcgg cacgtaacgc cgctgctcca caagagggat
    18181 gccaggtctt tattaaataa tctgcgtctt gcatcacaaa agcgcagtaa cgcgaacgca
    18241 tgagatgttc tggatcaggt gcaacctttt caccagacac ataagggtgg caacataggc
    18301 tatactcgac agcactacca cagggacaaa gctgagacac aaataatctc cctggaaaca
    18361 ataacggcgt attaaccgcc tgagtagcac tatgttaacc gagcagtagc gatgtggcta
    18421 cgattgcatt ccaggggaat cttgcgggaa taatgagaaa gataaaaata gggctggcgc
    18481 tgggatctgg cgcggcgaga ggttggtcgc atattggcgt tattaatgcg ctaaaaaaag
    18541 tgggtattga aattgatatc gttgcaggat gttcaattgg ttcgctggtg ggcgctgcct
    18601 atgcatgcga tcgattatct gcgctggaag attgggtgac ctctttcagt tattgggatg
    18661 ttttacgcct gatggatctc tcctggcagc gcggtgggtt actgcgcggc gagcgtgtct
    18721 tcaatcaatg ccgcgaaata atgccggaaa cagagatcga aaattgttcc cgtcgctttg
    18781 cggctgttgc caccaattta agtacgggac gtgaattatg gtttactgaa ggcgatctcc
    18841 atcttgctat tcgcgcatca tgcagtattc caggactcat ggcacctgtt gcacataacg
    18901 gctactggct ggttgatgga gcagtagtta acccaattcc tatttccctc acgcgtgcat
    18961 tgggggctga tattgtgata gcggtcgacc tgcagcacga tgctcatttg atgcaacaag
    19021 atttgctctc ctttaatgtc agtgaagaaa atagcgagaa tggtgaatct ttgccgtggc
    19081 atgcgcgtct gaaagaaagg ttaggcagta taacaacacg tcgggcggtg acagcgccaa
    19141 cggcaacaga gattatgacc acttctatcc aggtgctgga gaaccgcctt aaaaggaacc
    19201 gcatggcagg tgatccgccc gatattctga ttcaacctgt ttgcccgcaa atatctacgc
    19261 ttgatttcca tcgcgcgcac gctgccattg cggccggaca gctggcagtg gaaaggaaaa
    19321 tggacgaact tttgccgttg gtacgcacca acatttgacc agaattttta tctacactta
    19381 agttaattct gacaggcgca ggtggcaata gcatgccact attgagtaaa gccagtcagg
    19441 ggagagaaca tgacgcagcc attggtcgga aaacagattc tcattgttga agatgagcag
    19501 gtatttcgct cgcttctgga ttcatggttt tcctcattgg gagcgacaac ggtactggcg
    19561 gctgatgggg tggatgccct tgagttgctg ggaggtttca ctccagacct gatgatatgt
    19621 gatatcgcga tgccacgaat gaacgggctt aaactgctgg agcatatacg taacagaggc
    19681 gaccagaccc cagttctggt gatatctgcc actgaaaata tggcagatat tgccaaagcg
    19741 ttacgtctgg gcgttgaaga tgttttgctg aaaccagtta aagatctgaa tcgcttgcgc
    19801 gagatggttt ttgcctgtct ctatcccagc atgtttaatt cgcgcgttga ggaagaggaa
    19861 aggctttttc gcgattggga tgcaacggtt gataaccctg ccgcagcggc gaaattatta
    19921 caggaactac aaccgccggt tcagcaggtg atttcccatt gccgggttaa ttatcgtcaa
    19981 ttggttgccg cggacaaacc cggcctggtg cttgatattg ccgcactttc ggaaaacgat
    20041 ctggcatttt attgccttga tgtcacccga gctggacata atggcgtact tgctgccttg
    20101 ttattacgcg cattgtttaa cggattatta caggaacagc ttgcacacca aaatcaacgg
    20161 ttgccagagc tgggcgcgtt attgaagcag gtaaaccatt tactccgtca ggccaatctg
    20221 ccggggcagt ttccgctatt agttggctat tatcatcgcg aactgaaaaa tctcattctg
    20281 gtttctgcgg gtctgaatgc gacgttaaat accggcgaac accaggtgca aatcagtaat
    20341 ggtgttccgt taggcacttt aggtaacgct tatttgaatc aattgagcca gcgatgcgat
    20401 gcctggcaat gccaaatatg gggaaccggt ggtcgactgc gcttgatgtt gtctgcagaa
    20461 tgagcaaacg ataacgcgga ctaaatttgc attacctgct aatgtcggct ggtggtacta
    20521 tcgtcgccat tcgtataagt aattgtctta attatgctaa ctcgcctcct tttcagaact
    20581 tagccccttc ggggtgctga tatactggga tgcgatacag aaatatgaac acgttcaaaa
    20641 cacgaacagt ccaggagaat ttaaatggct gccattaata cgaaagtcaa aaaagccgtt
    20701 atccccgttg cgggattagg aaccaggatg ttgccggcga cgaaagccat cccgaaagag
    20761 atgctgccac ttgtcgataa gccattaatt caatacgtcg tgaatgaatg tattgcggct
    20821 ggcattactg aaattgtgct ggttacgcac tcatctaaaa actcgattga aaaccacttt
    20881 gataccagtt ttgaactgga agcaatgctg gaaaaacgtg taaaacgtca actgcttgat
    20941 gaagtgcagt ctatttgtcc gccgcacgtg actattatgc aagttcgtca ggggctggcg
    21001 aaaggcctgg gacacgcggt attgtgtgct cacccggtag tgggtgatga accggtagct
    21061 gttattttgc ctgatgttat tctggatgaa tatgaatccg atttgtcaca ggataacctg
    21121 gcagagatga tccgccgctt tgatgaaacg ggtcatagcc agatcatggt tgaacaggtt
    21181 gctgatgtga ccgcatatgg cgttgtggat tgcaaaggcg ttgaattagc gccgggtgaa
    21241 agtgtaccga tggttggtgt ggtagaaaaa ccgaaagcgg atgttgcgcc gtctaatctc
    21301 gctattgtgg gtcgttacgt acttagcgcg gatatttggc cgttgctggc aaaaacccct
    21361 ccgggagctg gtgatgaaat tcagctcacc gacgcaattg atatgctgat cgaaaaagaa
    21421 acggtggaag cctatcatat gaaagggaag agccatgact gcggtaataa attaggttac
    21481 atgcaggcct tcgttgaata cggtattcgt cataacaccc ttggcacgga atttaaagcc
    21541 tggcttgaag aagagatggg cattaagaag taacatccat atcggtgtta tccacgaaac
    21601 ggcgttgaac aatcgacgcc gtttttttat agcttattct tattaaattg tcttaaaccg
    21661 gacaataaaa aatcccgccg atggcgggat ttttagcaat tgcaatctac aaaagattat
    21721 tgcttgatca ggaaatcgtc gagggattta ccttgctcat ccattgcttt tttgattaca
    21781 gctggagtac ggccctggcc agtccaggtt ttagtttcgc cgttttcgtc aacatagcta
    21841 tattttgccg gacgctgagc acgtctagct ttggtgccgg atttaacggc agcaaggcta
    21901 ttcagcagtt cgttcgggtc aataccgtca gcgatcagca tttcgcgata ttgctgcagt
    21961 ttacgagtgc gctcttcaac ttcagcagca gccgcgcttt cttcttcgcg acgttcgtta
    22021 acaacaactt ctaatttttc cagcatttct tccagcgttt caagtgtaca ttctcttgcc
    22081 tgcgcacgaa gagtacggat gttgttcaga attttaagtg cttcgctcat tgtagtaatc
    22141 tcaaacttat attggggtgg tttgttgagg taataataga gccttaaatt cagttgtgca
    22201 atagccagga atgtaaggaa ttcaaaattg ttctttattt tgtgccgcca ataaatatct
    22261 tttcataaaa ttagccagaa aagacgcggc atatagccct atttacaccg atgatttcgc
    22321 agcacgtgag gttaaaactt cctgattcat gtcacatttt atggggagat tatcgtaggc
    22381 tgacggcctt tcagtcttct gtattagttg tgtttacgag aattccctat taagcgaatg
    22441 atgaaaagta gaacagtcgc aataagagca tggacttagt attgcactat ctcctggagg
    22501 tcaacagagg gctattactt gcgcaacagg ttaaagattg tgaatagtta ccagcagtca
    22561 tttacccgct tataacaagc gaggcagttg taatgatagc tcagaaggat tatgcaaggc
    22621 ttcgtaaggg agaacgcata tacccacttc tgtgcatact gttgagctga aaaactgacg
    22681 aattatgata aactccagcc aactttattt catatcattg agggcctgtg gctgatggca
    22741 cagctatatt tctactattc cgcaatgaat gcgggtaagt ctacagcatt gttgcaatct
    22801 tcatacaatt accaagaacg cggcatgcgc actgtcgtat atacggcaga aattgatgat
    22861 cgctttggtg ccgggaaagt cagttcgcgt ataggtttgt catcgcctgc aaaattattt
    22921 aaccaaaatt catcattatt tgatgagatt cgtgcggaac atgaacagca ggcaattcat
    22981 tgcgtactgg ttgatgaatg ccagttttta accagacaac aagtatatga attatcggag
    23041 gttgtcgatc aactcgatat acccgtactt tgttatggtt tacgtaccga ttttcgaggt
    23101 gaattattta ttggcagcca atacttactg gcatggtccg acaaactggt tgaattaaaa
    23161 accatctgtt tttgtggccg taaagcaagc atggtgctgc gtcttgatca agcaggcaga
    23221 ccttataacg aaggtgagca ggtggtaatt ggtggtaatg aacgatacgt ttctgtatgc
    23281 cgtaaacact ataaagaggc gttacaagtc ggctcattaa cggctattca ggaaaggcat
    23341 cgccacgatt aataagaatt tctttactga cagggtgagc agggcacttt tatcctgtca
    23401 gttcgtttta cgcacttctt ccgggctata tacccttctc ggcagttttt taacgccgct
    23461 atacgcctca cagggctctt aagcaccgac gttgacttgt gacctgtaaa gtacaatatc
    23521 cctgtgttta ggcgttatac atcgtcgcaa atatgatgaa ggctaatgct gtcggtttat
    23581 ggaaaagttg ctttgggtaa acaaaaaata cggccccaga agggcaatgc cgttcactta
    23641 agaggagcgg cactatgttt cacaggataa cgggtttttg atatcttaac cgacctcggc
    23701 cttgatggtc gggggcgttt tgttatgaac accacttcca gagcaccccg aagatgctcc
    23761 agtcgtttcg ggatggtccc tggtgacgcc gtgtttccca gctctatcat ttctgatgcg
    23821 atattcttcc acgcaggcag tagccagtgg cggttacaac ccttctggtt cagcgttagc
    23881 agcaggtctt cgctgtaaaa aagcttatca aacaacgtaa tagagttatc cgggatggtg
    23941 gcgagcatgg agtgggccag cacagtttcg ctctgccggt aaggtgcggt cacggcattc
    24001 agcagaatgt gacttcccag gttcattaag gccaccagac gcataaccgg gtaggcgttc
    24061 tgccgcttag tggatgtgtt ggcagaccca taatattcac gcagctcggg tttatcaggt
    24121 gtcctgaact gtgcgccatc aatggcaaaa agttgcaggc cgtgccagtc atccttcagg
    24181 taacgttccg cgcccctgtc tgtgcggtct ggcggaagag ccattccact ggggcggccc
    24241 ccacgcgctg acgcgcttgg gtgacagcgc tgcgggccag caggttcatc cccgcttcgc
    24301 catccgcgct caggttcaga cggcgaacaa catcggtaat tggctcattg caccaccatc
    24361 cagataacca tgtcccccgg taaacgacgg cggcgaacgg tcgcatgagc agaaagcgtc
    24421 aggcagtgtt gtatccactc ggtgggaagg tgttctgcaa atagttgtgc agagggcgga
    24481 ggcataagcg gatggtcact gaaatcgagc agatcattga gaagtggcat aagaaaacgg
    24541 ctccctgttg tggaagccgt tatagtgcct cagtttaagg atcggtcaac taatccttaa
    24601 ctgatcggca ttgcccagaa ggggccgttt atgttgccag acagcgctac tgattaagcg
    24661 gattttttcg cttttttctc agctttagcc ggagcagctt ctttcttcgc tgcagtttca
    24721 ccttctacat aatcacgacc gtagtaggta tccagcagaa tctgtttcag ctcggagatc
    24781 agcgggtaac gcgggttagc gccggtgcac tggtcatcga atgcatcttc agacagttta
    24841 tccacgttcg ccaggaagtc tgcttcctga acgccagctt cacggataga tttcggaata
    24901 cccagttcag ctttcagcgt ttccagccat gccagcagtt tctcgatctt agcagcagta
    24961 cggtcgcccg gtgcgctcag acccaggtgg tcagcaattt cagcataacg acggcgagcc
    25021 tgcggacggt catactggct gaatgcagtc tgcttggtcg ggttgtcgtt cgcgttgtag
    25081 cgaataacgt tacaaatcag cagggcgttt gccagaccgt gcggaatatg gaactgggaa
    25141 cccagtttgt gcgccattga gtgacataca cccaggaagg cgttcgcaaa cgcgataccc
    25201 gcgatagtcg ctgcactgtg aacacgttca cgcgctaccg gatttttaga cccttcgtgg
    25261 taggacgctg gcagatattc tttcagcagt ttcagtgcct gcagagcctg accatcagag
    25321 aactcagatg ccagtacaga aacataagct tccatggcgt gagttactgc gtccagacca
    25381 ccgaaagcac acagggactt cggcatgtcc ataaccaggt tggcgtcgac aatcgccata
    25441 tccggagtca gcgcatagtc tgccagcgga tatttctgac cagtagcgtc gtcagttaca
    25501 accgcaaacg gagtgacttc agaacctgta ccagaagtgg tggtgacagc gatcattttc
    25561 gctttcacgc ccattttcgg gaacttgtag atacgtttac ggatatccat aaagcgcagc
    25621 gccagctctt cgaagtgagt ttccggatgt tcgtacataa cccacatgat cttcgcggcg
    25681 tccatcgggg aaccaccacc cagcgcgata atcacgtctg gtttgaagga gtttgccagt
    25741 tctgcacctt tacgaacgat gctcagggtc gggtccgctt ctacttcgaa gaagacttca
    25801 gtttcaacgc ctgctgcttt cagtacggaa gtgatctgat cagcataacc attgttgaac
    25861 aggaagcggt cagtcacgat gagcgcacgt ttgtggccat cagtaatcac ttcatccagc
    25921 gcgattggca gggagccacg gcggaagtag atagatttcg gaagtttgtg ccacaacatg
    25981 ttttcagctc gcttagcaac ggttttcttg ttgatcaggt gtttcggacc aacgttttca
    26041 gagatggagt taccacccca agaaccacaa cccagagtca gggaaggtgc gagtttgaag
    26101 ttatacaggt caccgatacc accctgagac gctggggtgt taatcaggat acgagccgtt
    26161 ttcattttct gaccgaagta agaaacgcga gccggttggt tatcctggtc agtgtacagg
    26221 caagaggtat gaccgatacc gcccatagca accagtttct ctgctttttc taccgcgtct
    26281 tcgaaatctt tagcgcggta cattgccaga gtcggggaca gtttttcatg tgcgaacggt
    26341 tcgctttcat caacaacggt cacttcaccg atcagaatct tggtgttttc tggtacagag
    26401 aagcctgcca gttcagcaat tttataggct ggctgaccaa cgatagccgc gttcagcgca
    26461 ccgtttttca ggataacatc ctgaacagct ttcagctctt taccctgcaa cagatagccg
    26521 ccgtgggttg caaaacgttc acgtacagcg tcataaacag agtcaacaac aacaacagac
    26581 tgttcagaag cacagattac gccgttgtcg aaggttttgg acatcagtac agatgcaact
    26641 gcacgtttga tatcagcagt ttcatcgata acaactggag tgttgcccgc gcctacaccg
    26701 atagctggtt taccggagct gtatgcggct ttaaccatgc ccggaccacc agtcgcgagg
    26761 atcaggttga tgtctgggtg gtgcatcagt gcgttagaca gttcaacaga aggttgatcg
    26821 atccagccga tcagatcttt cggagcaccg gcagcgatag cagcctgcag aacgatatca
    26881 gccgctttgt tggtggcatc ttttgcacgc gggtgcgggg agaagataat ggcgttacgg
    26941 gtcttcagac tgatcagcga tttgaagata gcagttgaag tcgggttagt ggtcggaacg
    27001 ataccgcaaa taataccgat tggttcagcg atagtgatgg taccaaaagt gtcgtcttca
    27061 gacagaatac cacaggtttt ttcatcttta taggcgttgt agatatattc agaagcaaag
    27121 tggtttttga tcactttatc ttcgacgata cccatgccgg attcggcaac ggccattttc
    27181 gcgagtggga ttcgagcatc tgcagcagcc agagcggcgg cgcggaagat tttgtctact
    27241 tgctcttgag tgaaactggc atattcacgc tgggctttct ttacacgctc tacgagtgcg
    27301 ttaagttcag cgacattagt aacagccata atgctctcct gataatgtta aactttttta
    27361 gtaaatcatc tgctcgaata cgagagtata gtcagtgcgg tgatgatttg cttaacctat
    27421 gaaaatcaaa agcttactcg cgctcacact cactgtgatt tactaaaaga gtttaaacat
    27481 tagagttatt atctctaatg cgtcacttcc aggtggcgta agcaagatta ctcacttctg
    27541 ggtactgatt acgtgatcca aatcaaattt tttgcaaagc tgacaccttt cagcatcgct
    27601 tttcgccatt atagctaaca gttaataaat tgtagtatga tttggtggct acattagcat
    27661 gttttgcaca actagataac aataacgaat gatagcaatt ttaagtagtt aggaggtgaa
    27721 aaatgctgtc aaaaggcgta ttgtcagcgc gtcttttcaa ccttatttat ggctaacatt
    27781 atccggcttt tgcttcggag ctaaccgtga ttcagacctt ttttgatttt cccgtttact
    27841 tcaaattttt catcgggtta tttgcgctgg tcaacccggt agggattatt cccgtcttta
    27901 tcagcatgac cagttatcag acagcggcag cgcgaaacaa aactaacctt acagccaacc
    27961 tgtctgtggc cattatcttg tggatctcgc tttttctcgg cgacacgatt ctgcaacttt
    28021 ttggtatatc aattgattcg ttccgtatcg ccgggggcat cctggtggtg acgatagcga
    28081 tgtcgatgat cagcggcaag cttggcgagg ataaacagaa caagcaagaa aaatcagaaa
    28141 ccgcggtacg tgaaagcatt ggtgtggtgc cattggcgtt gccgttgatg gcggggccag
    28201 gggcgatcag ttctaccatc gtctggggta cgcgttatca cagcattagc tatctgtttg
    28261 gtttctttgt ggctattgca ttgttcgctt tatgttgctg gggattgttc cgcatggcac
    28321 cgtggctggt acgggtttta cgccagaccg gcatcaacgt gattacgcgt attatggggc
    28381 tattgctgat ggcattgggg attgaattta tcgttactgg tattaagggg attttccccg
    28441 gcctgcttaa ttaattcctt tcaaatgaaa cggagctgcc atgctccgtt tacttcgtca
    28501 ttatttttac t
//
LOCUS       DAJVUJ010000036        28214 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.036, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000036 DAJVUJ010000000
VERSION     DAJVUJ010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28214
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.036"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(<1..846)
                     /locus_tag="OQQ48_001455"
     CDS             complement(<1..846)
                     /locus_tag="OQQ48_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HCS1952275.1"
                     /translation="MVKKPQQGTIFAAAQRSDSYFVKCINMLSLYEKIKIRLIILFLL
                     AALSFIGLFFIINYQLVSERAVKRADSRFELIQKNVGYFFKDIERSALTLKDSLYLLK
                     NTEEIQRAVILKMEMMPFLDSVGLVLDDNKYYLFSRRANDKIVVYHQEQVNGPLVDES
                     GRVIFADFNPSKRPWSVASDDSNNSWNPAYNCFDRPGKKCISFTLRINGKDHDLLAVD
                     KIHVDLNWRYLNEYLDQISANDEVLFLKQGHEIIAKNQLAREKLIIYNSEGNYNIIDS
                     VDTEYI"
     gene            complement(946..1857)
                     /gene="gsiD"
                     /locus_tag="OQQ48_001456"
     CDS             complement(946..1857)
                     /gene="gsiD"
                     /locus_tag="OQQ48_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HCS1952276.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1860..2780)
                     /gene="gsiC"
                     /locus_tag="OQQ48_001457"
     CDS             complement(1860..2780)
                     /gene="gsiC"
                     /locus_tag="OQQ48_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HCS1952277.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILSSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2798..4336)
                     /gene="gsiB"
                     /locus_tag="OQQ48_001458"
     CDS             complement(2798..4336)
                     /gene="gsiB"
                     /locus_tag="OQQ48_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HCS1952278.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILVH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQAALLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(4356..6227)
                     /gene="gsiA"
                     /locus_tag="OQQ48_001459"
     CDS             complement(4356..6227)
                     /gene="gsiA"
                     /locus_tag="OQQ48_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HCS1952279.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLCVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(6214..7179)
                     /gene="iaaA"
                     /locus_tag="OQQ48_001460"
     CDS             complement(6214..7179)
                     /gene="iaaA"
                     /locus_tag="OQQ48_001460"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCS1952280.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFARGMERVSPAIFSTPLRYEQLLAARKEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIGHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            7383..8618
                     /gene="moeA"
                     /locus_tag="OQQ48_001461"
     CDS             7383..8618
                     /gene="moeA"
                     /locus_tag="OQQ48_001461"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HCS1952281.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            8618..9367
                     /gene="moeB"
                     /locus_tag="OQQ48_001462"
     CDS             8618..9367
                     /gene="moeB"
                     /locus_tag="OQQ48_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HCS1952282.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLIVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHNDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEYDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(9443..10105)
                     /gene="fsa"
                     /locus_tag="OQQ48_001463"
     CDS             complement(9443..10105)
                     /gene="fsa"
                     /locus_tag="OQQ48_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCS1952283.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKNPRQALDCLLAGCESITLPLDVAQQMISYPAVDAAVAKFEQDWLGAFGRT
                     SI"
     gene            10236..11135
                     /locus_tag="OQQ48_001464"
     CDS             10236..11135
                     /locus_tag="OQQ48_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HCS1952284.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEIATALLQACHEAGIHTAVETC
                     LHVPWKYITPSLPYIDLFLADLKHIADAPFKQWTDGNAAKVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APDLLDFAQQYACQKGLTATLRG"
     gene            11141..13573
                     /locus_tag="OQQ48_001465"
     CDS             11141..13573
                     /locus_tag="OQQ48_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HCS1952285.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLAIAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQTLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGNVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            13719..14534
                     /gene="ybiV"
                     /locus_tag="OQQ48_001466"
     CDS             13719..14534
                     /gene="ybiV"
                     /locus_tag="OQQ48_001466"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HCS1952286.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIEFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKAVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIAFDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            14686..15951
                     /locus_tag="OQQ48_001467"
     CDS             14686..15951
                     /locus_tag="OQQ48_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HCS1952287.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSTSRPEIYPIYWSQTQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     HEDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(16192..17784)
                     /locus_tag="OQQ48_001468"
     CDS             complement(16192..17784)
                     /locus_tag="OQQ48_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HCS1952288.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLMKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            18003..18923
                     /gene="ldtB"
                     /locus_tag="OQQ48_001469"
     CDS             18003..18923
                     /gene="ldtB"
                     /locus_tag="OQQ48_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCS1952289.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(18982..20100)
                     /locus_tag="OQQ48_001470"
     CDS             complement(18982..20100)
                     /locus_tag="OQQ48_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HCS1952290.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKVLQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLASLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVILPAN"
     gene            complement(20097..20564)
                     /gene="mntR"
                     /locus_tag="OQQ48_001471"
     CDS             complement(20097..20564)
                     /gene="mntR"
                     /locus_tag="OQQ48_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HCS1952291.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGTK"
     gene            20750..20878
                     /gene="mntS"
                     /locus_tag="OQQ48_001472"
     CDS             20750..20878
                     /gene="mntS"
                     /locus_tag="OQQ48_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HCS1952292.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLILLSMLCDMVNNKPQQDKPSDK"
     gene            21150..22733
                     /gene="opgE"
                     /locus_tag="OQQ48_001473"
     CDS             21150..22733
                     /gene="opgE"
                     /locus_tag="OQQ48_001473"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCS1952293.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(22782..23297)
                     /gene="ompX"
                     /locus_tag="OQQ48_001474"
     CDS             complement(22782..23297)
                     /gene="ompX"
                     /locus_tag="OQQ48_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HCS1952294.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(23350..23415)
                     /locus_tag="OQQ48_001475"
     CDS             complement(23350..23415)
                     /locus_tag="OQQ48_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1952295.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            23650..24537
                     /gene="rhtA"
                     /locus_tag="OQQ48_001476"
     CDS             23650..24537
                     /gene="rhtA"
                     /locus_tag="OQQ48_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HCS1952296.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            24836..25339
                     /gene="dps"
                     /locus_tag="OQQ48_001477"
                     /gene_synonym="pexB"
     CDS             24836..25339
                     /gene="dps"
                     /locus_tag="OQQ48_001477"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HCS1952297.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            25743..26489
                     /gene="glnH"
                     /locus_tag="OQQ48_001478"
     CDS             25743..26489
                     /gene="glnH"
                     /locus_tag="OQQ48_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HCS1952298.1"
                     /translation="MKSVLKVSLAALTLVFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            26628..27287
                     /gene="glnP"
                     /locus_tag="OQQ48_001479"
     CDS             26628..27287
                     /gene="glnP"
                     /locus_tag="OQQ48_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HCS1952299.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            27284..28006
                     /gene="glnQ"
                     /locus_tag="OQQ48_001480"
     CDS             27284..28006
                     /gene="glnQ"
                     /locus_tag="OQQ48_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HCS1952300.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
BASE COUNT         6978 a         7538 c         6970 g         6728 t
ORIGIN      
        1 aatatactcc gtatcgacag aatcaataat attataatta ccttcgctat tataaataat
       61 cagtttttca cgagcgagtt gattcttggc aatgatctca tgaccttgtt tcaaaaatag
      121 aacttcatca ttagcgctga tttgatcaag atactcgttc aaataccgcc agtttaaatc
      181 gacatgaatt ttatctaccg ctaacaaatc gtgatctttg ccgttgatac gtagcgtaaa
      241 agagatacat tttttacccg gacgatcaaa gcaattgtat gccggattcc agctgttgtt
      301 agagtcatct gaagccaccg accacggtcg tttcgatggg ttaaaatcgg caaaaataac
      361 ccgccctgac tcgtcgacaa gcggtccatt tacttgttcc tgatgataaa caacgatttt
      421 atcattcgcc ctccgtgaaa agagataata tttattatca tcaagtacca gtcccaccga
      481 gtctaaaaat ggcatcattt ccattttcag aatcacggcg cgttgaatct cctctgtatt
      541 ttttaataaa tacagtgagt cctttaatgt cagggccgaa cgttcaatat ctttaaagaa
      601 atagccaacg tttttctgaa taagttcaaa gcggctatcg gcacgtttta ccgcgcgttc
      661 cgataccagt tgatagttaa tgatgaaaaa aagaccaata aatgacagtg ctgccaataa
      721 aaataaaatt atcagcctta tctttatctt ttcgtataaa ctcagcatgt taatacattt
      781 aacaaaataa ctatctgaac gctgtgccgc tgcaaaaata gtcccttgct gcggcttttt
      841 aaccaaatta cccgattcta caaaatcagc agaaagttac aacacttgct tagggcaata
      901 atttcctgtg aggtgattac cctttcaagc aatattcaaa cgtaactatc ctttaatttt
      961 cggatccagc gcatcgcgta aaccatcgcc caacaaattg aacgccagta cggtcagaaa
     1021 aatagccagg gccggaaaaa cagcgacatg cggcgcgata accatatccg ctcgtgcctc
     1081 attgagcatt gctccccact ctggtgtcgg cggctgcgca ccgaggccga gaaatgacag
     1141 gctggcggca gagataatcg aggtgccaat gcgcatggtg aaaaacacca cgatagacga
     1201 gacggtccca ggcaagatat gacgcaataa aatggtcata tcgctggcac caatactgcg
     1261 tgctgactca ataaaggttt gctgtttcaa caccagcgtg ttgccgcgca ccagtcgggc
     1321 aaacgcgggg atggaaaaaa tggcgactgc gataatcacg ttagcaatgc cgcttcccaa
     1381 caccgcaaca acagcgatcg ccagtaaaat acccgggaag gcaaacagca catcgcaaat
     1441 gcgcatgatc agccgatccc accagccttc ataatatcca gccaacaagc ccagcaacgt
     1501 cccgatcgcc gcaccgataa acacggcaaa cacgcccgcc gccagcgaga tttgcgcacc
     1561 aaccaggaca cggctgaaaa tgtcgcgccc cagtgaatcg acgccaaacc agtgctgcaa
     1621 agaaggtccg ttattcagat tgtcataatc aaaataattt tcggcgtcat agggagcgat
     1681 ccagcgtgca aaaatggcca ccacaatcaa taaaataacg aataatgcgg cggtcatcgc
     1741 catatgctga cggcgaaatc gtcgccagaa ttcatgccac ggtgtacgca cctggtcagg
     1801 tttgaccagt ggcatggcgt ttaacaccgc ctgacgtcgc cagttaaata gtcgcatcct
     1861 tacttgtacc tgatagccgg gttaatggcg gcgtaaagca catccaccac taagttgata
     1921 agaataaatt ccagcgagaa aagcagaatt tccgcctgaa tcaccgggta atcgcgcatt
     1981 tctacggagt caacgagtaa gcgtccaagt ccgggccagt tgaaaacttt ctccaccacg
     2041 atagaaccac cgagcaaaaa gccaaactgt aagcccatca tggtcactac cgggatcatc
     2101 gcgttacgta gcccgtgttt gaggacaacc caggtttcgc tcaccccttt cgccctcgcg
     2161 gtacgcatat aatcttcgct taaaacatcg acaaacgacg cgcgggtaaa gcgcgccatc
     2221 acggctgcca ctgccgcgcc gagcgtcagg gaggataaaa tgtagtgctg ccaactgtct
     2281 gctcccacgg taggcagcca gcccagttca acggagaata cctgaattaa aagcatcccc
     2341 agcgcaaatg ccggaaacga gatccccgac accgcaatgg tcatactcaa tcgatccggc
     2401 caacgattac gccagacggc ggcgataatt cccgccgcca taccaaatat caccgcccag
     2461 accatactgg ttatggtcag ccacagcgtt ggcataaagc ggctggcaat ctcatcggca
     2521 accggacgac gcgacaccat cgacaggcca aaatccccct gcacagcatt gctgatatag
     2581 tgccagaact ggtgatacag cggctgatcc aaccccagct gctgacgcac cagttctata
     2641 acctgcgcat cagcttcggg cccggcaatc aatcgcgccg gatcgccggg cagcatatgc
     2701 acaaataaaa acaccagcac cgagacgata aacagcgtcg gaatcaaccc cagtaagcgt
     2761 ttgataacgt aattaagcat tccactccct gcgttgctta ttgcaaatcc gcgtcttcaa
     2821 agctgaagcc ggtgtctggc atgatccaaa aaccggtcag gtttttactg tgtgccgaca
     2881 ccagtttttc taccaccagc gggatccacg gcgactcttg ccagatgata tcctgcgccg
     2941 ccttatacaa gcgggtcttt tccgccggat cattagtttt cagtgcctga gccaggaagt
     3001 cgtccacctg tttattgctg taaaatgcgg tattaaacag cgtcggtggc cagttttgcg
     3061 aggcgaacag tggcgatagc gcccagtccg cttcgccggt tgaagccgac cagccagtgt
     3121 agaacatccg cacgccgctc tctttttgcc ctttaccttc aacttctgcc gcccgctgtc
     3181 cggcatccat cgcggtcacc tgggctttaa tcccgacctg cgctaactgc tgctgggtaa
     3241 attgcagcac tttctgcgcg gtgctgtggt tatgtgacga ccacagcgtg gtactgaaac
     3301 cgttgggata tcccgcctct ttcagtaatt cgcgcgcttt cactggatcg taaggccacg
     3361 gtttataact ttgcgcgtag gcgatacttg gcggtaccac accagtagct ggcgttgcat
     3421 agcccgcaaa ggcaactttc accagcgccg gacggttaat ggcgtaattc agcgcctcac
     3481 ggaccttcgg gttatcgaac ggcttttgcg tcacgttcat actgatataa cgctgcataa
     3541 ttgacggact ggccatcaac tcgatatttt tgtttttctc cagcagtgcg gcctgctcgt
     3601 agggaatggg gaaagcaaac tgcgcttcac cggtttgcag cattgccgcg cgggtgttgt
     3661 tatccgccac cggacgccag gttatgctgt ccagtttggg caatcctggc tgccagtaac
     3721 ccgcgaattt tttcaccttc acaaaatcgg tctgattcca ggtatccagt tcatacggtc
     3781 cggttcccac cggatgaaaa ccaatctcct tgccatattt ttccagcgct gccggtgaaa
     3841 tcatcgcggt cgccggatgg acaagaatat taataaacgc tgagaacggc tgtttgaggg
     3901 taatctttac cgttgtcgga tcgatcgcct ccgttttggc aatattctta tacaggttat
     3961 agcgtttaag atgattcgcc ggatcgctgg cccggtccag attcgctttc accgccgcgg
     4021 cgttgaaatc ggtgccatcc tggaatttaa ttccttcccg caatttcacg gtgtaagtaa
     4081 tgccgtcatc ggaaacggta taactctccg ccagcacgtt tttcagtttc atctctttat
     4141 ccagaccgaa cagcccctgg taaaacgatt tcgctacggc ctgagataac gtgtcattcg
     4201 cgtcatacgg atcgagcgtg gtgaaattcg atcctaccgc caccaccaca tctttggcag
     4261 cgaatgcaca agatgccatc aacgctgtcg caatgcccag cgccactaac ccactacggt
     4321 gtacagctct tgccatttta ttctccgcct gaatgttatc tacgcatgaa tgcgtattct
     4381 gattgtggtt gtgcgacgta atgccccggc ccgacgcatt gcaacgagac ggctgccacc
     4441 tcttcgccac gcagatgaat attgctggga agatcgtccg acagcagtac acgctgcggt
     4501 cgttgtcggg acggttcagc gaccggaact gccgccagta atttacgcgt ataaggatgc
     4561 tgcgggtttt cgaagaccgc gcgccgtgga ccaatttcaa caatttgccc gagatacatc
     4621 accgccacac gatgactaat ccgctctacc acggccatat cgtgggagat aaacagatac
     4681 gcaatgccga aatcacgctg gagatcgagc aacaagttga taatctgccc gcgaatagaa
     4741 acatccagcg ccgaaacggc ttcgtcggca atgatcactt ttggattcaa tgccaacgcg
     4801 cgagcaatgc agatgcgctg gcgctgaccg ccggaaaact catgcgggta acgccaggca
     4861 tgttcaggta acaggcccac gcgctccagc aaccacgcaa cgcgtgcagc cgcgtcttta
     4921 cctggcaata aaccgtgtac acacagcggt tcgataatcg aatcaccgat ggtctgacgt
     4981 gggtccagcg aagcgtaagg gtcctgaaaa ataaactgaa tatcccggcg caatgcctga
     5041 agtttgccag gtgacaaggt atcgattcgc tgaccgttaa agataatttc gccgccctgc
     5101 gattcgacca ggcgcagcaa cgcccgcccg gtagtggatt taccgctgcc agactcgccc
     5161 accagcgata gcgtttcgcc aggccagaga tcaaaactga ctttctcaac ggcatgcact
     5221 tcccgcgtta cgcgattcaa caaaccgctg cgcaaaggga aacgggtgac aagattacgc
     5281 actcgtaaaa caggttcgcc atccaccacc gttttctgct cgatgggggg ttcctgtttc
     5341 gctggatgtt caagcgatat caatgggaaa cgtcggggat aatctaaccc tttcatcgca
     5401 ccaagttgcg gaacagcagc taacagcgca cgggtgtaag gatgttgcgg tgcatgaaaa
     5461 atctgttcga cggtacccgt ttccaccgcc tcgccctgat acatcaccag tacccgatcg
     5521 gctatctctg ccaccacgcc catatcgtgc gtgataaaga taacacccat cgacatctct
     5581 ttttgcaata ctttgattaa ttgcaggatc tgcgcctgaa tagtgacatc cagcgcggtg
     5641 gttggctcat cggcaatcag caccgccggg cggcatgaca gcgccatcgc aatcatcact
     5701 cgctggcgca tcccgccaga gagttgatgc ggataacgtg aaagaatggt ttgtgcctca
     5761 ggaatgcgta cctgatccag catccgcttc gcctcgacca tcgcttcttc acgactggcg
     5821 ttctgatgca gacgaattga ttcggcaatc tgttcaccca cagtaaatac cgggttcagc
     5881 gatgtcatcg gctcctgaaa tatcatcgcc atatccgcac cgcgaacatg gcgcatttgt
     5941 gcagcgctct gctcgctaag ttcaatcact tcgcgactgc gccgctgcaa cagcatttta
     6001 tcgcactgta ctaaaccgcc cgcctgttcc aacaggcgca tcaatgccaa cgcagtcact
     6061 gacttaccgg agccggattc gccaacaatt gccagcgtct caccgcgttg cagactaaaa
     6121 gagagattgc ggaccgcagc tattttctgc tggtcctgca taaaggcaat attcagattt
     6181 tcaaccgcca gcacattacc ggcatcaagt tcatcactgt gtggcaacgg tgtccccttt
     6241 ttcacggtag ataccggtgg ttggcgtatc gcctgcgtag ccccaggcgc gatacattcc
     6301 ttcggtgtta aacggtagcg cgacattccc ttcatggccg atagcgatta agccaccgct
     6361 accgccaagc gcagggagtt tttccattac tacccgctcg caggcttccg cgagacttaa
     6421 tccgccgtaa tccattaacg cggcgatgtc atatgccgcc agcgcgcgga tgaagacttc
     6481 gcccgtgccg gtacaagaaa ccgccacact ggcgttattg gcgtagcatc cggcacccac
     6541 taagggacta tcgccaactc gtccgggtaa tttattggtc attccgcctg tggacgtggc
     6601 tgccgccaaa ttgccgtcta aatccaacgc cacggccccc acggtgccca ttttctgttt
     6661 ttcatccagt ggcgcaccgc tatggtcgag gacggttgcc ccttccttgc gcgctgccag
     6721 tagttgttca taacgcaaag gcgtggagaa aatcgccggc gagacgcgct ccatgccacg
     6781 agcaaacgca aaattttctg ccccttcgcc aatcatcatc acatgcgggc tttgctccat
     6841 caccagccgg gcggcaagaa ccggattacg cagatgacta acgcccgcca ccgcaccggc
     6901 tttcagggtg ttaccatcca tcacacaggc gtccagttca tgggtttcat cacgcgtaaa
     6961 gacagcgcca attccggcgt taaacagtgg acactcttcc agcagacgca ccgcttccgt
     7021 caccacatcc agcgcacttt cgcccgcttc cagcattttc tgcccggttt caacaatggc
     7081 agacaacgcc tcaatgtagc gtaattcctg ttgcagactc atctgcgcgc ggctaattgc
     7141 acctgcgcca ccatgaattg caatgactgc tttgcccata gtattcgtcc attaagggtg
     7201 gctcgccact tttgctataa atatcagaat ttttatgaat tactcattca atttttgaat
     7261 atagaaagat ggatatggca tgtaaaggca ggagccaacg ctacgcccga ggtggcatac
     7321 agtctgtggc gtttttctga cataataggc aaattcgatt ttgcctccgc aggagtgttt
     7381 tcatggaatt taccaccgga ttgatgtcgc tcgacaccgc gcttaatgag atgctttctc
     7441 gcgtcacccc actgaccgcc caggaaacgc tgccactggt acagtgtttt ggtcgtattc
     7501 tggcgagcga tgtcgtttcg ccgcttgatg ttccggggtt tgataactcc gcaatggacg
     7561 gctacgcggt gcgtttagcc gatattgcct ccgggcaacc gctgcccgtt gccggtaaat
     7621 cctttgccgg tcagccgtat catggtgaat ggcctgcggg cacctgcatt cgtattatga
     7681 ccggtgcgcc ggtgccggaa ggctgcgaag cggtggtgat gcaggagcag actgaacaaa
     7741 cggacaatgg cgtgcgtttt actgctgaag tgcgtagcgg gcaaaatatt cgccgtcgcg
     7801 gtgaagatat ctctgcaggt gcggttgttt tcccggcggg aactcgcctg actaccgcag
     7861 agctgccagt aattgcttca ctggggattg ccgaagttcc ggtgattcgt aaagtgcgtg
     7921 tagcgctttt ttctaccggt gatgaactcc agttgcccgg tcagccgctg ggtgacggcc
     7981 aaatctacga caccaaccgt ctcgccgtac acctgatgtt agaacagctg ggttgcgagg
     8041 taatcaactt agggattatc cgcgacgatc cccatgccct gcgcgccgca tttattgaag
     8101 ccgacagcca ggcggatgta gtgatcagtt ccggcggtgt ttcagtgggt gaagcggatt
     8161 acaccaaaac gattcttgaa gagctggggg agatcgcctt ctggaagctg gcgattaaac
     8221 caggtaaacc gttcgcgttc ggtaaactca gcaatagctg gttctgcggc ctgccgggca
     8281 acccggtttc agcgacgctg accttctatc aactggtaca gcctttgctg gcaaaactaa
     8341 gcggcaacac cgccagcggc ctgcccgcgc gccagcgcgt acgcacagcg tcccgcctga
     8401 aaaaaacgcc aggacgtctt gatttccagc gcggcgtgct gcaacgcaac gccgacggtg
     8461 aacttgaagt gacgaccacc ggacatcagg gttcacatat atttagctcc tttagtctcg
     8521 gcaactgctt tatcgtgctg gaacgcgatc gcggcaatgt ggacgtgggc gaatgggtgg
     8581 aagtagaacc gtttaacgcg ttgttcggag gcctgtaatg gcggaactca gcgatcagga
     8641 gatgctgcgc tacaaccggc aaatcattct gcgcggtttt gattttgacg gtcaggaggc
     8701 gctaaaagat tctcgtgtgc tgatcgtcgg cctcggtgga ctcggctgcg cagcttcgca
     8761 gtatctggca agcgccggtg tcggcaacct gacgctgctc gacttcgaca cggtttcgct
     8821 ctcgaatctg caacgccaga cactgcacaa tgatgccacg gtcgggcaac cgaaggtgga
     8881 atccgcccgt gacgccctga cgcggatcaa cccacatatc gcgattacgc cagtcaatgc
     8941 actgctagat gacgcagaac ttgcagcatt gattgctgaa tacgatctgg tgctcgactg
     9001 tacggataac gttgcggtac gtaatcaact gaacgcaggc tgttttgccg cgaaggtgcc
     9061 gctggtttcc ggcgcggcaa ttcgtatgga agggcaaatc acggtcttca cttatcagga
     9121 cggcgaaccg tgctatcgct gccttagccg tttgtttggc gagaacgcgt taacctgcgt
     9181 agaagcaggc gtgatggcac cgttgatcgg cgtaattggt tcgttgcagg caatggaagc
     9241 gatcaaactg ctggcaggtt atggcaaacc ggccagcggg aaaatcgtca tgtacgatgc
     9301 gatgacctgt cagtttcgcg aaatgaaact gatgcgtaat ccggggtgtg aggtgtgcgg
     9361 gcagtaattg ccacctccag gccagataag acgcggcact ccgccacgtc atcagcaatt
     9421 tatgaggatg cagaacgtcc ggttaaatcg acgttctgcc aaacgctccc agccagtcct
     9481 gttcaaactt cgccacagcg gcatcaaccg ccggatagct aatcatctgt tgtgccacat
     9541 ccagtggcag cgtaattgat tcacatcctg ccagtaagca gtccagcgcc tgacgcgggt
     9601 ttttgaaact cgctgccagc actttcgcct gcggcgcatg cattttcaat aactggtgta
     9661 agtcggtcac agtctgaatg ccgctaccgc cctgagcatc aatacgatta acgtaaggcg
     9721 caacatattc cgcacctgcc agcgccgaca gcagcccttg tgctgcgcca tataccgcgg
     9781 ttcccagcgt tggaatccct tccgctttta acatcttaat agctgccagc ccctcggcgg
     9841 tcaccggaac tttcaccacg atatccgcaa taatagaacg caatttacgt gcgtcattca
     9901 ccatcccttc agcagtggta gccattacct gggcaaacag acgcccctga ccgcccatcg
     9961 cttcatgaag ttgcggaagc acaacatcca gcggtttttt acccgcggcg ataatgcttg
    10021 ggttagtggt cacacccgcc agcggaaaaa tacgtgacag cgccttcact gcaacaacgt
    10081 ctgaagtatc cagatacagt tccataacca tcctcaaaat gtactttttg tctgaatgta
    10141 gactatcacg ggaaaactct ctgaacagtt gacgcacatc aatccaactt tcattcgaaa
    10201 gtattttaat gtttgaacga aacagagagg caactatgat tttcaatatt cagcgctact
    10261 cgacccatga tggccccggt atccgcacgg tcgtatttct taaaggctgt tcgctgggct
    10321 gccgctggtg tcagaacccg gaaagccgcg cccgcacgca ggatctgctg tatgacgcac
    10381 gactgtgtct ggaaggctgc gagctgtgcg ctaaggcagc gccggaagtg attgagcgcg
    10441 cattgaatgg tttgcttatt catcgggaaa agttaacccc ggagcatctg acggcgctaa
    10501 ccgactgctg cccgacacag gcgttaaccg tgtgtgggga agtgaaaagc gttgaggaga
    10561 tcatggcgac cgttctgcgc gataaaccgt tttacgatcg cagcggcggc ggcttaacgc
    10621 tttcgggtgg tgaacccttt atgcagccgg aaatagcgac ggcgctactg caagcctgcc
    10681 acgaggcagg cattcatact gcggtagaaa cctgtctgca tgtgccgtgg aaatatatca
    10741 ccccttctct gccctatatc gatctgtttc ttgccgattt aaaacacatt gccgacgcgc
    10801 cgtttaaaca gtggaccgac ggtaacgccg ccaaggtgct ggataacctg aaaaaactcg
    10861 ccgcagcggg caaaaaaatc attatccgcg tgccgctgat tcagggcttt aatgccgacg
    10921 aaacctctgt aaaagccatt accgattttg ccgccgacga gctgcacgtt ggcgaaattc
    10981 attttctgcc ctaccacacg ctgggcatca acaaatatca cttacttaat ctgccctatg
    11041 acgccccgga aaaaccgctt gatgcgccag acctgctcga ctttgcccag cagtatgcct
    11101 gccagaaagg gttaaccgcg accttacgag gataacaacc atgaccacac tgaaactgga
    11161 cacgctcagc gaccgcatta aagcgcacaa aaatgcgctg gtgcatattg tgaaaccgcc
    11221 ggtctgtacc gagcgcgcgc agcactatac cgagatgtat caacaacatc tcgataagcc
    11281 gatcccggta cgtcgcgcgc tggctctggc gcatcacctg gcgaatcgca ccatctggat
    11341 caagcacgat gagttgatca tcggcaacca ggcaagcgag gttcgcgctg cgccgatctt
    11401 cccggaatat accgtctcgt ggatcgaaaa agagattgat gatctggcag atcgtcccgg
    11461 tgctggtttt gcggtgagcg aagagaacaa acgcgttctg catgaagtgt gcccgtggtg
    11521 gcgcggtcag acggtacagg atcgctgcta cggcatgttt accgatgagc aaaaaggtct
    11581 gctggcgacc ggaatcatta aagcggaagg caatatgacc tccggcgatg cgcacctggc
    11641 ggtgaatttc ccgctgctgc tggaaaaagg tcttgatggt ctgcgcgaga aagtagcgga
    11701 acgtcgctcg cgcatcaatc tgacggtact ggaagatttg cacggcgaac agttcctgaa
    11761 agcgattgat atcgtgctgg tggcagtcag cgaacacatt gaacgttttg cagccctggc
    11821 gcgtgaaatg gccgcgaccg aaacccgcga aagccgtcgc gatgaactgc tggcgatagc
    11881 agaaaactgc gatcttatcg cccaccagcc gccgcagact ttctggcagg cgctgcaact
    11941 gtgttacttc atccagttga tattgcagat cgaatctaac ggtcactcgg tatcgtttgg
    12001 tcgtatggac cagtatctct acccgtacta tcgccgagac gttgaactca accagacact
    12061 ggatcgcgaa cacgccatcg aattgctgca tagctgctgg ctgaaactgc tggaagtgaa
    12121 caagatccgc tccggctcac actcaaaagc ctctgcggga agtcctctgt atcagaacgt
    12181 caccattggc ggacaaactc tggttgatgg tcaaccaatg gacgcggtga atccactctc
    12241 ttacgcgatc ctcgaatcct gcggtcgcct gcgttccact cagcctaacc tcagcgtgcg
    12301 ttaccatgca ggaatgagta atgatttcct cgacgcctgc gtacaggtga tccgttgcgg
    12361 cttcgggatg ccggcgttca acaacgacga aatcgtgatc ccggaattta ttaaactcgg
    12421 tattgaaccg caggacgctt acgactacgc agcgattggt tgtatcgaaa ccgccgtcgg
    12481 tggcaaatgg ggctatcgct gtaccggcat gagctttatc aacttcgccc gcgtgatgct
    12541 ggcggcgctg gaaggcgggc gtgatgccac aagcggcaac gtgttcctgc cacaagaaaa
    12601 agcgttgtcg gcaggcaact ttaacaattt cgatgaagta atggacgcgt gggatacgca
    12661 aatccgttat tacacgcgta aatcaatcga aatcgaatat gtcgtcgaca ccatgctgga
    12721 agagaacgtg cacgatattc tctgctcggc gctggtggat gactgtattg agcgagcgaa
    12781 aagtatcaag caaggcggcg cgaaatatga ctgggtttct ggcctgcagg ttggcattgc
    12841 caacctcggc aacagcctgg cggcagtgaa gaaactggta tttgaacagg gcgcgattgg
    12901 tcagcaacag ctggctgccg cactggcgga tgacttcgat ggcctgactc atgagcaact
    12961 gcgtcagcgg ctgattaacg gtgcgccgaa gtacggcaac gacgatgata ctgtcgatac
    13021 gctgctggct cgcgcttatc agacctatat cgacgaactg aaacagtacc ataatccgcg
    13081 ctacggtcgt ggtccggttg gcggcaacta ttacgcgggt acgtcatcta tctccgctaa
    13141 cgtaccgttt ggtgcgcaaa ctatggcaac gccggacggg cgtaaagccc acaccccgct
    13201 ggcagaaggc gctagtccgg cctctggtac tgaccatctc ggccctactg cggtcattgg
    13261 ctcggtgggt aaactgccta cggcagcgat tctcggcggc gtgttgctca accagaaact
    13321 gaatccggca acgctggaga acgaatctga caagcagaag ctgatgatcc tgctgcgcac
    13381 cttcttcgag gtgcataaag gctggcatat tcagtacaac atcgtttccc gcgaaacgct
    13441 gctggaggcg aaaaaacatc cggatcagta ccgcgatctg gtagtgcgtg tcgcgggtta
    13501 ttccgccttc ttcaccgcgc tctctccaga cgctcaggac gatatcatcg ctcgtactga
    13561 acatatgctg taatctcttc ggcccggctg cggcgtcgcc gggctaaatc attcacatca
    13621 tcaatttcat ccttactttc attcgaaata taatttgtgc tctgcgtcac attgttatta
    13681 cactgttacg gtttcatttt cgagccagga gcacacctat gagcgtaaaa gttatcgtca
    13741 cagacatgga cggtactttt cttaacgacg ccaaaacgta caaccaacca cgttttatgg
    13801 cgcaatatca ggaactgaaa aagcgcggca ttgagttcgt tgtcgccagc ggtaatcagt
    13861 attaccagct tatttcgttc tttcctgagc taaaggatga gatctctttt gtcgcggaaa
    13921 acggcgcact ggtttacgaa catggcaagc aactgttcca cggcgaactg acccgacatg
    13981 aatcgcggat tgttattggc gagttgctaa aagataagca actcaatttt gtcgcctgcg
    14041 gtctgcaaag tgcatatgtc agcgaaaatg cccccgaagc atttgtcgca ctgatggcaa
    14101 aacactacca tcgcctgaaa gctgtaaaag attatcagga gattgacgac gtactgttca
    14161 agttttcgct caacctgcca gatgaacaaa tcccgttagt gatcgacaaa ctgcacatag
    14221 cgttcgatgg cattatgaaa cccgtcacca gtggttttgg ctttatcgac ctgattattc
    14281 ccggtctaca taaagcaaac ggtatttcgc ggttactgaa acgctgggat ctgtcaccgc
    14341 aaaatgtggt agcgattggc gacagcggta acgatgcgga gatgctgaaa atggcgcgtt
    14401 attcctttgc gatgggcaat gctgcggaaa acattaaaca aatcgcccgt tacgctaccg
    14461 atgataataa tcatgaaggc gcgctgaatg tgattcaggc ggtgctggat aacacatccc
    14521 cttttaacag ctgacctctc taccggagcc gtgcgctccg gttttccctt ctttttacat
    14581 cctgtacatc acatcaaatc attaaacttg cattaactta tttataactt atattgtcac
    14641 atgcaaatca taatggtttc gaatgaaagt taatgagagg tggttatggc ttctactttt
    14701 accagcgaca cattgccagc cgatcacaaa gcagctatcc gtcagatgaa gcacgcgctg
    14761 cgggcgcagc ttggcgacgt ccagcagatc tttaatcagc taagcgatga cattgccacg
    14821 cgagtggctg aaatcaacgc actcaaagca cagggcgatg ccgtctggcc ggtgctgtct
    14881 tatgccgata tcaaagcagg tcatgttact gcagagcagc gcgaacagat taaacgtcgc
    14941 ggttgtgcgg tgataaaagg ccatttcccc cgcgaacaag cgctaggctg ggatcagtcg
    15001 atgctggact atctggaccg caaccgcttt gacgaggtct acaaaggccc cggcgataat
    15061 ttcttcggga cgctcagcac ttcacgtccc gagatttacc ccatctactg gtcgcagacg
    15121 caaatgcagg cccgccagag tgaagaaatg gcgaatgcgc agtcgtttct caatcgtctg
    15181 tggacatttg aaagtgatgg aaagcaatgg tttaacccgg atgtgagcgt catctaccct
    15241 gaccgtatcc gccgccgtcc gcccggaacg acctccaaag gtcttggagc gcataccgac
    15301 tccggggcgc tggaacgctg gctgcttcca gcgtatcagc gcgttttcgc caacgtcttt
    15361 aatggcaatc tggcgcaata tgatccctgg catgcggcac atcgtacgga agttgaagag
    15421 tacacggtgg acaacaccac caaatgttcc gtgtttcgga cattccaggg ctggacagcg
    15481 ctctctgata tgctgcctgg tcaggggttg ctgcacgttg tgcccattcc tgaagccatg
    15541 gcgtacgtac tgttacgtcc gctgcttgat gatgtgccgg aggatgaact gtgcggcgta
    15601 gcgcccggaa gagtgttgcc ggtatcagag caatggcatc cactgttaat tgaggcgtta
    15661 accagcattc caaaactcga ggccggagac tccgtctggt ggcactgcga cgtcatccat
    15721 tccgttgccc ccgttgaaaa tcaacagggt tggggcaacg tgatgtacat tcctgcggca
    15781 ccgatgtgcg agaaaaatct tgcctacgcg cacaaggtga aggccgcact ggaaaaaggc
    15841 gcatcgccgg gcgacttccc gcacgaggac tatgaaacaa actgggaagg acgctttacg
    15901 cttgccgacc tcaacattca cggtaagcga gcgttgggca tggatgtttg agagttaatg
    15961 gaagtgaatt gatgtgggat taagaaaatc gattcgacgt tactcagatt gctgacaagt
    16021 gcgctttctt aatgcctgat gcggcgttaa cgccttatct ggcctacaaa gtcctgcaga
    16081 ttcaatatat tacaggtact gcgtaggcct gataagcgta gcgcatcagg ctattttgca
    16141 tttgtcatca gccgccgtca ccccgacagt atcggggtga cggataatca cttactcgat
    16201 ccctttacta cgcaggtaat cttcgtaatt accgctaaag tcgatcacgc gttccggggt
    16261 gatttccaga atgcgggtcg ccagggagct tacgaactca cggtcgtgtg aaacaaagat
    16321 cagcgtgccc tgatacagtt ccagcgccat gttcagtgac tcgatggatt ccatatccag
    16381 gtggttggtc ggttcgtcca tgatcagaat gttcggcttc tgcatcatta acttaccaaa
    16441 cagcatccgc cctttttccc caccggaaag cactttagct ggctttttga tgtcgtcctg
    16501 gctgaacagc aaacgaccga gaatgctgcg taccgcctgc tcgtcatcgc cttcctgctt
    16561 ccactgactc atccattcga acacggtcag atcattttca aactcatatt cgtggtcctg
    16621 ggcgtagtaa ccaatgcgcg cgttttcaga ccattttacg gtgccgctgt ccggttgcag
    16681 atcgcccacc agcgttttca tcagcgttga tttaccgacg ccgttggtac ccagtaccgc
    16741 aagtttttca ccgacctcca gcagcaggtt gagattttta aacagtggac cgttatcaaa
    16801 ccctttggtc agaccttcca cttccagcgc gttacggaac agtttcttat cctgttcaaa
    16861 acggatgaac gggttctgac ggctggaggc tttcacctct tccagtttga ttttatcaat
    16921 ctggcgcgcg cgcgaagttg cctggcgaga tttcgaggcg ttggcgctaa agcggctaac
    16981 gaaagattgc aactcagcaa tctgcgcttt cttcttggcg ttatcggcca gcagacgttc
    17041 acgcgcctgg gtcgccgccg tcatgtactc atcgtagtta cccggataaa cgcgcagctc
    17101 gccgtaatcc agatccgcca tgtgggtaca gaccatgtta aggaagtgac ggtcgtgcga
    17161 gatgatgatc atggtgctgt cacgctcgtt cagcacctgt tccagccagc gaatggtgtc
    17221 gatgtcgagg ttgttggtcg gttcgtcgag caggagaata tccggatcag caaacagcgc
    17281 ctgcgccaga agcacacgca gcttccagcc aggagcaact tcactcatcg ggccgtagtg
    17341 ctgttccact ggaattccca cgccaagcaa caattcaccg gcgcgagctt ccgcagagta
    17401 accgtccatt tcgccgtatt taacttccag atcggccact ttatagccgt cttcctcact
    17461 catttccggc aaagcataga tgcggtcacg ctcctgcttc acttcccaca actctttatg
    17521 ccccatgatc accgtatcca gcacagtgaa ctcttcaaag gcaaactgat cctgacgcag
    17581 cttaccaatg cgctcgttgg gatcgaggga aacgttaccc aaagtcggct ctaagtcgcc
    17641 gccgaggatc ttcataaagg tggatttacc actaccgttc gcgccaatca ggccgtaacg
    17701 gttgccgccg ccaaatttga cggaaatgtt ttcaaacaac ggcttactgc cgaactgcat
    17761 ggtgacgtta ctggaaacta acacaggcgt atcctgaaaa gagatatgac aaaccgcgca
    17821 ttatgccata agtctgaaag aagatcctgc tcctcgctta aggtctacac tcaagccaaa
    17881 ggcaaaaaat gtgatttcgt acacatctga tttcactgtg agctggaatg aacttataat
    17941 gcgcttccaa tactctaata ttctcaaccc aatggcctgc caggcacaaa atctcgctta
    18001 acatgaatat gaaattgaaa acattattcg cagcggcctt cgctgttgtc ggcttttgca
    18061 gtaccgcctc tgcggtaact tatcctctgc caaccgacgg gagtcgcctg gttggtcaga
    18121 atcaggtgat caccattcct gaaggtaaca ctcagccgct ggagtatttt gccgcggagt
    18181 accagatggg gctttccaat atgatggaag cgaacccggg tgtggatacc ttcctgccga
    18241 aaggcggtac tgtactgaac attccgcagc agctgatcct gccggatacc gttcatgaag
    18301 gcatcgtcat taacagtgca gagatgcgtc tgtattacta tccgaaaggg accaacaccg
    18361 ttatcgtgct gccgatcggc attggtcagt taggcaaaga tacgcctatc aactggacca
    18421 ccaaagttga gcgtaagaaa gcaggcccga cctggacgcc gaccgcaaaa atgcacgcag
    18481 agtaccgcgc tgcgggcgaa ccgcttccgg ctgtcgttcc ggcaggtccg gataacccga
    18541 tgggtctgta tgcactctac atcggtcgcc tgtatgctat ccatggcacc aacgccaact
    18601 tcggtatcgg cctgcgtgta agtcatggtt gtgtgcgtct gcgtaacgaa gacatcaaat
    18661 tcctgttcga gaaagtaccg gtcggtaccc gcgtacagtt tattgatgag ccggtaaaag
    18721 cgaccaccga gccagacggc agccgttata ttgaagtcca taacccgctg tctaccaccg
    18781 aagcccagtt tgaaggtcag gaaattgtgc caattaccct gacgaagagc gtgcagacag
    18841 tgaccggtca gccagatgtt gaccaggttg ttcttgatga agcgattaaa aaccgctccg
    18901 ggatgccggt tcgtctgaat taatcttcaa aacttaaagc aaaaggcgga ctcataatcc
    18961 gcctttttta tttgccagac cctagttggc cgggagtata actaacaaaa catatcccac
    19021 caacgccgcc cacaacagca tcggtattga atagagatgg aaacgccacc agatgcggcg
    19081 atcgttcgcc atacgtagcg caatcaaact tgccagcgat ccgggtaata acccaaagcc
    19141 acctacgttt accgcccatg ccagtaataa agacggcggc acatagttca gcaacaatat
    19201 ggtactcggc acgttactga tcacctgcga taaaccgatt gccgttaacc ataacccggg
    19261 ttcagataga tgactcacgt tacccaacac gccttgcaac gctggaagct gggtcagtaa
    19321 atggacgtcg ataaacatcg ccataaacac cagcagcagc gtccagtcca cactgagcac
    19381 cacgcgacgt gccagcagcg caaagcctgc cgccacaatc accagtcccc acagctcttg
    19441 tttgaactcc agcgccgtta gaaagacgat atacagcccc agacaactcc acaccagccg
    19501 cggtttccac tccggtgttt gcacccccgt atggtattgc agcacctttc cagggaaaca
    19561 acaccagcac aggagcagga gcgtcagcat cattgcgcca gccagcggtg ccatttgggc
    19621 aataaatccg gcaaacgaaa gaccagaacg tccccagata agaatatttt gcgggttgcc
    19681 aattggcgtc agtagcgaac cagcattgac tgccagcgcc tcaaaaataa tcagacgatt
    19741 aaccgggatc tcacacagtc tttttagcgt gatagtcagc ggaacaacaa taaacagcgc
    19801 gacatcgttg gtcagaaagg tagaaagcag cgccgccgcc agcaccataa acatcgccag
    19861 ccgacgctcc gtagcaaagc ggcgcaccat tttgcgcccc agcacatcaa aataaccgct
    19921 taactccaca cctttggtca gcagcatcag gccgcttaag gtgatgatgg tgtgccagtc
    19981 gatagcagca ggccaggatt tcggtgcaaa gggcacgaaa aagcttaatc cgataccaac
    20041 aagaattaat aactgaaaaa aacgatcgcc ttgcagcgtg cgtaaaaaag gcaggctcat
    20101 ttggtaccgt gtttctgggt aaacaaacga aaagcgtcga gcgtctcttc actaacatgg
    20161 tgctccatgc cttccgcgtc gcgacgggcg atttccggac tgacgcccaa caccaacaag
    20221 aaattttcga ctatctgatg tcgctcgcgg ctttcctgcg ccagcttctc tccttctgcc
    20281 gttaaaaaca cgccacgcca ggggatcatt tcaatcagcc ccatggttgc cagccgctta
    20341 agcattttag ccaccgtcgg ttgcgaaact cccagacgag cagccatgtc cacctgacga
    20401 gcttccccca cttccctgat caagtcagaa atcagctcaa cgtaatcatc aataagctcg
    20461 cgccgatgcg cctctctgac ctggcggaac ccttcaacgt gctcttccac gttcactaac
    20521 tgcgtcactt tttttgctgt tggcgtacct gcgcgacgac tcattgtgct tcctcattat
    20581 ggtgacgcat acatagcgtt ctcatttcgt gcgcacattg taaaccagag ttgcgaaggt
    20641 acaaaaaatt aacgttttag caatagctat ataatatagc ctgtgctata tctgtatgta
    20701 atgcaatcat ccctcaagga tcgacgggat tagcaagtca ggaggtctta tgaatgagtt
    20761 caagaggtgt atgcgcgtgt ttagtcattc tccctttaaa gtacggttaa tactgctctc
    20821 tatgttgtgc gatatggtca acaacaaacc gcagcaagat aaaccttccg ataaatagcg
    20881 gcgtcgcggt acgccgcttc actcctgctt tcatgcaggc ataacgcgtt ttggcctgaa
    20941 aaaccccact ttttgtcgga tttgcaatcc ccttcgcaaa agatttgttc gtcagtagtt
    21001 gacctgaacg gcggctcgct ctatcttctt gcagccctgc gtatattgcg gctcgcggat
    21061 gcggacccct ttccactctt cacgcactct tgcaggtatt gacccttgac gccagggtaa
    21121 gcacatggcg tttgttacga tagtggcata tgaatttaac cctcaaagaa tcgcttgtta
    21181 cccgtagccg ggtatttagc ccgtggactg cgttctactt tttacagtcg ctattaatta
    21241 acctcggctt aggttacccc ttcagtttgc tctacaccgc tgcgtttacg gctattttgc
    21301 ttttgctatg gcgaacattg cctcgcgtac aaaaagttct ggtcggtgtc agttcgctgg
    21361 tggcggcttg ttatttccct tttgctcagg cctacggcgc gcctaatttc aatacattgc
    21421 tggcattgca ctccaccaat atggaagagt cgaccgaaat cctgacgatt tttccgtggt
    21481 acagctacct ggtcggctta tttatttttg cgctcggcgt aatagcaatc aggcgaaaaa
    21541 aagagaatga aaaagcgcgc tggaatacct tcgacagcct gtgtctggta ttcagtgtgg
    21601 cgacattttt tgttgctccc gtgcaaaacc tggcctgggg tggcgtattt aaactgaaag
    21661 atactggcta tccggtattt cgttttgcta aggatgtcat cgtcaataat aacgaggtga
    21721 ttgaagagca agaacggatg gcaaaacttt ccggaatgaa agatacctgg acggtcactg
    21781 ccgttaagcc gaagtatcag acctatgtgg tggtgatcgg tgaaagcgcg cgtcgcgatg
    21841 ccctcggtgc ctttggcggt cactgggaca ataccccgtt tgccagcagc gttaacggtt
    21901 tgatatttgc tgactacatt gccgccagtg gctccacgca gaaatcgctt ggcttaacgc
    21961 tcaatcgcgt agtcgatggc aaaccacagt ttcaggataa ctttgtcacc ctggcaaatc
    22021 gcgcgggctt ccagacctgg tggttttcca accagggcca aatcggcgaa tacgatactg
    22081 ctatcgccag catcgccaaa cgtgcagatg aagtgtactt cctgaaagaa ggtaattttg
    22141 aagcagataa aaacacgaaa gacgaagcgt tactggatat gaccgctcaa gtgctggcgc
    22201 aagagcactc gcaaccgcag ctgattgttc tgcatctgat gggctcgcat ccgcaggcct
    22261 gcgacaggac acaaggcaaa tacgaaacct ttgtgcaatc gaaagaaacg tcgtgctatc
    22321 tctataccat gacgcaaacg gacgatttac tgcgcaagct gtacgatcag ttacgcaaca
    22381 gcggcagcag cttctctctg gtttactttt ctgaccacgg tctggccttt aaagagcgcg
    22441 gcaaagacgt gcaatacctt gcccatgatg ataaatatca gcaaaatttc caggtgcctt
    22501 ttatggtcat ttccagcgac gataaagcgc atcgggtgat taaagcccgc cgctcagcca
    22561 atgacttctt aggctttttc tcgcagtgga cgggaattaa agcgaaggaa ataaatatca
    22621 aatacccgtt tatatctgag aagaaagccg ggccgatata catcaccaac ttccagttac
    22681 agaaggtgga ttacaaccat ctcggaaccg atattttcga cccgaaacct taaaaacaaa
    22741 aatccgcccc gagaggcgga ttttttatat caccaaagtg attagaagcg gtaaccaaca
    22801 ccggcaatcc aggtgcctac gtcaacgcta cgaatacggc tctgctcgta agagaagtcc
    22861 agagcaacgt tttccatcgg gttgaactgc agaccagcac cgtaggagaa accgtagtcg
    22921 ctggtgtcgt gtttgtaggt cgggtattca gtggtctgga atttaccata acccacaccc
    22981 actacaccgt agatgcttgc ccagtcgtta atgcggtaag ccggaccagc agtgatgccg
    23041 tagtactggt ttttgttgta gtcaccagag cttgcagtac gacttttctc ggtgtaagtg
    23101 aaagaaccga tcacacccag cgggctgttg tcttcttcat agcggtattt caggttgaaa
    23161 ccgcccattt tgttcatttg gccctgagcg tcgctctgtg cgtaaccgcc agttacggta
    23221 gaagtcgcag ctacggaagt acctgcggtg aaagccagaa ctgcggccag tgctgaaaga
    23281 catgcaattt ttttcataac cacctcaaat gtgattcaaa taagtcctaa gttttaaata
    23341 tatcaaaaat taatgggaaa ctcttcgcga tttgtgatgt ctaacgggcc atttcatgta
    23401 acagaacgtt tccatacacc gctatccatc taaatttaaa tcactttttc agagaactgc
    23461 gtaagtatta cgcatgtttt ccctgtcatt catccagatt attcctaatc accagactaa
    23521 tgattccatc aatcctggcg cattttagtc aaaacggggg aaaatttttt caacaaatgc
    23581 tcaaccagca ttgggtatat ccagtacact ccacgcttta cttaagtcta gatatttgtg
    23641 ggagaaagga tgcctggttc attacgtaaa atgccggtct ggttaccaat agtcatattg
    23701 ctcgttgcca tggcgtctat tcagggtgga gcctcgttag ctaagtcact ttttcctctg
    23761 gtgggcgcac cgggtgtcac tgcgctgcgt ctggcattag gaacgctgat cctcatcgcg
    23821 ttctttaagc catggcgact gcgctttgcc aaagagcaac ggttaccgct gttgttttac
    23881 ggcgtttcgc tgggtgggat gaattatctt ttttatcttt ctattcagac agtaccgctg
    23941 ggtattgcgg tggcgctgga gttcaccgga ccactggcgg tggcgctgtt ctcttctcgt
    24001 cgcccggtag atttcgtctg ggttgtgctg gcggttcttg gtctgtggtt cctgctaccg
    24061 ctggggcaag acgtttccca tgtcgattta accggctgtg cgctggcact gggggccggg
    24121 gcttgttggg ctatttacat tttaagtggg caacgcgcag gagcggaaca tggccctgcg
    24181 acggtggcaa ttggttcgtt gattgcagcg ttaattttcg tgccaattgg agcgcttcag
    24241 gctggtgaag cactctggca ctggtcggtt attccattgg gtctggctgt cgctattctc
    24301 tcgaccgctc tgccttattc gctggaaatg attgccctca cccgtttgcc aacacggaca
    24361 tttggtacgc tgatgagcat ggaaccggcg ctggctgccg tttccgggat gattttcctc
    24421 ggagaaacac tgacacccat acagctgctg gcgctcggcg ctatcatcgc cgcttcaatg
    24481 gggtctacgc tgacagtacg caaagagagc aaaataaaag aattagacat taattaaatt
    24541 tacatttctg catggttatg cataaccatg cagaatttct cgctactttt cctctacacc
    24601 gtctttatat atcgaattat gcaaaagcat atttattccg aaaattcctg gcgagcagat
    24661 aaataagaat tgttcttatc aatatatcta actcattgaa tctttattag ttttgttttt
    24721 cacgcttgtt accactatta gtgtgatagg aacagccaga atagcggaac acatagccgg
    24781 tgctatactt agtctcgtta attactggga cataacatca agaggatatg aaattatgag
    24841 taccgctaaa ttagttaaat caaaagcgac caatctgctt tatacccgca acgatgtctc
    24901 cgacagcgag aaaaaagcaa cagtagagtt gctgaatcgc caggttatcc agtttattga
    24961 tctttctttg attaccaaac aagcgcactg gaacatgcgc ggcgctaact tcattgccgt
    25021 acatgaaatg ctggatggct tccgcaccgc actgatcgat catctggata ccatggcaga
    25081 acgtgcagtg cagctgggcg gtgtagctct ggggaccact caagttatca acagcaaaac
    25141 cccgctgaaa agttacccgc tggacatcca caacgttcag gatcacctga aagaactggc
    25201 tgaccgttac gcaatcgtcg ctaatgacgt acgcaaagcg attggcgaag cgaaagatga
    25261 cgacaccgca gatatcctga ccgccgcgtc tcgcgacctg gataaattcc tgtggtttat
    25321 cgagtctaac atcgaataaa tccatcgctg atggtgcaga actttagtac ccgataaaag
    25381 cggcttcctg acaggaggcc gttttgtttt gcagcccacc tcaacgcact tatttagtgc
    25441 atccatctgc tatctccagc tgattaagta aattttttgt atccacatca tcacacaatc
    25501 gttacataaa gattgttttt tcatcaggtt ttacgctaaa taatcactgt gttgagtgca
    25561 caattttagc gcaccagatt ggtgccccag aatggtgcat cttcagggta ttgccctata
    25621 aatcgtgcat cacgtttttg ccgcatctct aaaaatcaat gagttgcaaa actggcacga
    25681 ttttttcata tatgtgaatg tcacgcaggg gatcgtcccg tggatagaaa aaaggaaatg
    25741 ctatgaagtc tgtattaaaa gtttcactgg ctgcactgac cctggttttt gcggtttctt
    25801 ctcatgccgc ggataaaaaa ttagttgtcg cgacggatac cgccttcgtt ccgtttgaat
    25861 ttaaacaggg cgataaatat gtgggctttg acgttgatct gtgggctgcc atcgctaaag
    25921 agctgaagct ggattacgaa ctgaagccga tggatttcag tgggatcatt ccggcactgc
    25981 aaaccaaaaa cgtcgatctg gcgctggcgg gcattaccat caccgacgag cgtaaaaaag
    26041 cgatcgattt ctctgacggc tactacaaaa gcggcctgtt agtgatggtg aaagctaaca
    26101 ataacgatgt gaaaagcgtg aaagatctcg acgggaaagt ggttgctgtg aagagcggta
    26161 ctggctccgt tgattacgcg aaagcaaaca tcaaaactaa agatctgcgt cagttcccga
    26221 acatcgataa cgcctatatg gaactgggca ccaaccgcgc agacgccgtt ctgcacgata
    26281 cgccaaacat tctgtacttc atcaaaaccg ccggtaacgg tcagttcaaa gcggtaggtg
    26341 actctctgga agcgcagcaa tacggtattg cgttcccgaa aggtagcgac gagctgcgtg
    26401 acaaagtcaa cggcgcgttg aaaaccctgc gcgagaacgg aacttacaac gaaatctaca
    26461 aaaaatggtt cggtactgaa ccgaaataat aacgctacac ctgtaaagcg cactggcagt
    26521 tccctctccc ctctggggag aggattaggg tgaggggcgc aaacccgctc cggggccatt
    26581 aattaccctg aatttgatta tttacaccac ggtaacagga acaacatatg cagtttgact
    26641 ggagtgccat ctggcctgcc attccgcttc tgattgaagg tgccaaaatg accctgtgga
    26701 tttcggtcct cggtctggct ggcggtctgg taatcggatt gctggcaggt tttgcacgca
    26761 ccttcggtgg ttggatagcc aaccacgtcg cgctggtctt tattgaagtg atccgcggca
    26821 cacctatcgt cgtccaggtg atgtttatct atttcgccct gccgatggcg tttaacgact
    26881 tacgcatcga cccatttact gcggcggtgg tcaccatcat gatcaactcc ggcgcgtaca
    26941 ttgcggaaat cacgcgtggt gcggtgctgt ctattcacaa aggttttcgt gaagcaggac
    27001 tggcgctcgg tctttcacgt tgggaaacca ttcgctacgt cattttaccg ctggcactgc
    27061 gtcgtatgct gccgccgctg ggtaaccagt ggatcatcag cattaaagac acctcgctgt
    27121 ttattgttat cggcgtggcg gaactgaccc gtcaggggca agaaattatt gccggtaact
    27181 tccgcgccct tgagatctgg agcgccgtgg cggtgttcta tctgattatt accctggtgc
    27241 tgagctttat tctgcgtcgt ctggaaagaa ggatgaaaat cctgtgattg aatttaaaaa
    27301 cgtctccaag cactttggcc caacccaggt gctgcacaat atcgatttga acattgccca
    27361 gggcgaagtc gtggtgatta tcgggccgtc cggttccggt aaatcgaccc tgctgcgctg
    27421 catcaacaaa ctggaagaaa tcacctccgg cgatctgatt gtcgatggcc tgaaggttaa
    27481 cgatccgaaa gttgacgagc gcctgattcg ccaggaagca ggtatggtgt tccagcagtt
    27541 ttacctcttc ccgcatctga cggcgctgga aaacgtcatg tttggtccgc tacgcgtgcg
    27601 tggcgcgaac aaagaagagg cggaaaaact ggcgcgtgag ctgctggcga aagtcggtct
    27661 ggcagaacgt gcacatcact acccttccga actttctggt ggtcaacagc agcgtgtggc
    27721 gattgcccgc gcgctggcgg tgaagccgaa aatgatgctg tttgatgaac cgacttccgc
    27781 tcttgacccg gaactgcgcc atgaagtgct gaaggttatg caggatctgg ctgaagaagg
    27841 gatgacgatg gtgatcgtga cccacgaaat cggttttgcc gagaaagtag cttcgcggct
    27901 gatctttatc gacaaaggcc ggattgcgga agatggcaat ccgcaggtgt tgatcaagaa
    27961 cccgccgagc cagcgcttgc aggaattttt gcagcacgtc tcttaataag acacattgcc
    28021 tgatgcgcta cgcttatcag gcctacagga tatctggcaa cttattaaaa ttgcatgaac
    28081 ttgtaggacg gataaggcgt tcacgccgca tccggcaaaa aagcccgcac gttgtcagca
    28141 atctgcttaa tatcccttcc tccctttcac ccgaaaggga ggcacaccag attcctctca
    28201 tttaaaatcg cccc
//
LOCUS       DAJVUJ010000037        27842 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.037, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000037 DAJVUJ010000000
VERSION     DAJVUJ010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27842
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.037"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..527
                     /locus_tag="OQQ48_001481"
     CDS             <1..527
                     /locus_tag="OQQ48_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCS1952301.1"
                     /translation="KYKIMANHHGIIFDEQRKRYIIGSITVSIDEMTNALDPVELAEI
                     INKKHHAVINGKKFKNSRAISCRSFNRYFSVSTDYRPKLEALLACSQMLGINQVVAHN
                     GNGGRECIGETGTVLGLNARDSKHAGRMFSMHNCQINPGAGPEITTPWKSYQHEKNRN
                     GLMPLIRRRTMLQL"
     gene            893..1960
                     /locus_tag="OQQ48_001482"
     CDS             893..1960
                     /locus_tag="OQQ48_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCS1952302.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHMRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            1957..4692
                     /gene="rbbA"
                     /locus_tag="OQQ48_001483"
     CDS             1957..4692
                     /gene="rbbA"
                     /locus_tag="OQQ48_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HCS1952303.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMSAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSTLSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDMWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            4692..5816
                     /locus_tag="OQQ48_001484"
     CDS             4692..5816
                     /locus_tag="OQQ48_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1952304.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            5889..6164
                     /locus_tag="OQQ48_001485"
     CDS             5889..6164
                     /locus_tag="OQQ48_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HCS1952305.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP"
     gene            6161..6520
                     /locus_tag="OQQ48_001486"
     CDS             6161..6520
                     /locus_tag="OQQ48_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HCS1952306.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLLEYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(6640..7041)
                     /gene="nikR"
                     /locus_tag="OQQ48_001487"
     CDS             complement(6640..7041)
                     /gene="nikR"
                     /locus_tag="OQQ48_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HCS1952307.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(7047..7853)
                     /gene="nikE"
                     /locus_tag="OQQ48_001488"
     CDS             complement(7047..7853)
                     /gene="nikE"
                     /locus_tag="OQQ48_001488"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HCS1952308.1"
                     /translation="MTLLNVSGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDETVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRATEKV"
     gene            complement(7850..8614)
                     /gene="nikD"
                     /locus_tag="OQQ48_001489"
     CDS             complement(7850..8614)
                     /gene="nikD"
                     /locus_tag="OQQ48_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HCS1952309.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKAVYPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            complement(8614..9447)
                     /gene="nikC"
                     /locus_tag="OQQ48_001490"
     CDS             complement(8614..9447)
                     /gene="nikC"
                     /locus_tag="OQQ48_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HCS1952310.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSH
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVSGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(9444..10388)
                     /gene="nikB"
                     /locus_tag="OQQ48_001491"
     CDS             complement(9444..10388)
                     /gene="nikB"
                     /locus_tag="OQQ48_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HCS1952311.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMAVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(10388..11962)
                     /gene="nikA"
                     /locus_tag="OQQ48_001492"
     CDS             complement(10388..11962)
                     /gene="nikA"
                     /locus_tag="OQQ48_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HCS1952312.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKLSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKTPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(12073..12660)
                     /gene="acpT"
                     /locus_tag="OQQ48_001493"
     CDS             complement(12073..12660)
                     /gene="acpT"
                     /locus_tag="OQQ48_001493"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HCS1952313.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(12715..13751)
                     /locus_tag="OQQ48_001494"
                     /pseudo
     CDS             complement(12715..13751)
                     /locus_tag="OQQ48_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
     gene            13883..15100
                     /locus_tag="OQQ48_001495"
     CDS             13883..15100
                     /locus_tag="OQQ48_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1952314.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKSWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(15104..15661)
                     /gene="dcrB"
                     /locus_tag="OQQ48_001496"
     CDS             complement(15104..15661)
                     /gene="dcrB"
                     /locus_tag="OQQ48_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HCS1952315.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAENNTTGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(15734..16399)
                     /locus_tag="OQQ48_001497"
     CDS             complement(15734..16399)
                     /locus_tag="OQQ48_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HCS1952316.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSILGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            16620..16865
                     /gene="tusA"
                     /locus_tag="OQQ48_001498"
     CDS             16620..16865
                     /gene="tusA"
                     /locus_tag="OQQ48_001498"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HCS1952317.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(16967..19165)
                     /gene="zntA"
                     /locus_tag="OQQ48_001499"
     CDS             complement(16967..19165)
                     /gene="zntA"
                     /locus_tag="OQQ48_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HCS1952318.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIAHQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQSATHPLAQAIVREAQVAALAIPAAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEYKVKAVTELNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(19239..19865)
                     /locus_tag="OQQ48_001500"
     CDS             complement(19239..19865)
                     /locus_tag="OQQ48_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000964717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HCS1952319.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYF"
     gene            20006..20365
                     /locus_tag="OQQ48_001501"
     CDS             20006..20365
                     /locus_tag="OQQ48_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HCS1952320.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(20368..20637)
                     /locus_tag="OQQ48_001502"
     CDS             complement(20368..20637)
                     /locus_tag="OQQ48_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HCS1952321.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(20627..21223)
                     /gene="rsmD"
                     /locus_tag="OQQ48_001503"
     CDS             complement(20627..21223)
                     /gene="rsmD"
                     /locus_tag="OQQ48_001503"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HCS1952322.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            21373..22866
                     /gene="ftsY"
                     /locus_tag="OQQ48_001504"
     CDS             21373..22866
                     /gene="ftsY"
                     /locus_tag="OQQ48_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCS1952323.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPVEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLF
                     RGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMG
                     EILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFELQGKSVMLAAGDTFRA
                     AAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSH
                     LMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDG
                     TAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            22869..23537
                     /gene="ftsE"
                     /locus_tag="OQQ48_001505"
     CDS             22869..23537
                     /gene="ftsE"
                     /locus_tag="OQQ48_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HCS1952324.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            23530..24588
                     /gene="ftsX"
                     /locus_tag="OQQ48_001506"
     CDS             23530..24588
                     /gene="ftsX"
                     /locus_tag="OQQ48_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HCS1952325.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTNFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            24833..25687
                     /gene="rpoH"
                     /locus_tag="OQQ48_001507"
     CDS             24833..25687
                     /gene="rpoH"
                     /locus_tag="OQQ48_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HCS1952326.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            25958..27061
                     /gene="livJ"
                     /locus_tag="OQQ48_001508"
     CDS             25958..27061
                     /gene="livJ"
                     /locus_tag="OQQ48_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HCS1952327.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(27260..27643)
                     /gene="panM"
                     /locus_tag="OQQ48_001509"
     CDS             complement(27260..27643)
                     /gene="panM"
                     /locus_tag="OQQ48_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HCS1952328.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
BASE COUNT         6590 a         7224 c         7740 g         6288 t
ORIGIN      
        1 ccaaatacaa aataatggca aatcatcatg ggataatttt tgatgagcag cgaaaacgtt
       61 acattatagg atcgataaca gtctctattg atgaaatgac caatgctctc gatccggtcg
      121 aattagcgga aattattaat aaaaagcacc atgctgtaat taatggcaaa aagtttaaaa
      181 atagccgggc tatctcttgt cgatccttta atcgttattt ttctgtttcg acagattaca
      241 gacctaaact tgaagcacta ttagcatgct cacaaatgtt aggtataaat caagttgttg
      301 cacataacgg taatggtgga agagaatgta taggtgaaac ggggacagtt ttaggtctta
      361 acgcccgtga cagtaaacat gctggaagaa tgttcagcat gcataattgc caaattaacc
      421 ctggtgcagg gcctgagata accactccct ggaagtctta ccagcatgaa aaaaacagaa
      481 atggacttat gcctttgatt agaaggcgca caatgttaca gctctgaacc atagacaaca
      541 tgaatattaa tgttactatc tggttgagac tgttgataaa acgtaaaaag gaatgctctg
      601 ttcctgcaac tgaacaggcg tgaaacgcga tccaacagga tggataccgt tttgtcagcc
      661 agttttatag atcacactta tgagtatgcg tgtttcattt acggccagga atgctgatga
      721 tgacaacatt actacccgtt ttcactaagc cttccccgtt ggcgctcaat gctctgcgcg
      781 ctggtcgtat ttgtcgtttc cttcttatcc cggatggaag aatccgctga acgtcgttcg
      841 gcggcagttg taacacgcgt cgtttgctta cagggattat ggcgggccga ttatggataa
      901 gagtaagcgc catctggcgt ggtgggttgt cggggcactg gcggtggctg ccgtcgtggc
      961 gtggtggctg ttgcgcccgg cgggtgtgcc ggaaggcttt gctgtcagca atgggcgcat
     1021 tgaagcgacg gaagtggata tcgccagcaa aattgccggg cgtatcgaca ccattctggt
     1081 gaaagaaggc cagtttgttc gcgaaggtga agtgctggcg aagatggata ctcgtgtgtt
     1141 gcaggaacag cgactggaag ccatcgcgca aatcaaagag gcacaaagcg ccgttgccgc
     1201 cgcgcaggct ttgctggagc aacgacaaag cgaaactcgc gccgcgcagt cgctggttaa
     1261 tcaacgccag gcagaactgg actccgtagc aaaacgtcat atgcgttccc gttcactggc
     1321 ccaacgaggg gctatttctg cgcaacagct ggatgacgac cgcgccgccg ctgagagcgc
     1381 ccgagctgcg ctggaatcgg caaaagctca ggtttctgct tctaaagccg ctatagaggc
     1441 ggcacgtacc aatatcattc aggcgcaaac ccgcgtcgaa gcggcacaag ccactgaacg
     1501 gcgcattgcc gcagatatcg atgacagcga actgaaagcc ccgcgtgacg gacgcgtgca
     1561 gtatcgggtt gccgagccag gggaagtgct ggcggcaggc ggtcgggtgc tgaatatggt
     1621 cgatctcagc gacgtctata tgactttctt cctgccaacc gaacaggcgg gcacgctaaa
     1681 actgggcggt gaagcacggc tgatcctcga tgccgcgcca gatctgcgta ttccggcgac
     1741 catcagtttt gtcgccagcg tcgcccagtt cacgccaaaa accgtcgaaa ccagcgatga
     1801 acggctgaaa ctgatgttcc gcgtcaaagc gcgtatccca ccggaattac tccagcagca
     1861 tctggaatat gtcaaaacgg gcttgccggg cgtagcgtgg gtgcgggtga atgaagaact
     1921 tccgtggcct gacgacctgg tggtgaggtt gccgcaatga cgcatctgga actggttccc
     1981 gtcccgcctg tcgcgcaact ggcgggcgtg agccagcatt atggaaaaac cgttgcgctg
     2041 aacaatatca ctctcgatat tccggcccgc tgtatggtcg ggctgattgg cccggacggc
     2101 gtcgggaagt cgagtttgtt gtcgttgatt tccggtgctc gtgtcattga gcagggcaat
     2161 gtgatggtgc tgggcggcga tatgcgcgac ccgaagcatc gccgcgacgt ctgcccgcgc
     2221 atcgcctgga tgccgcaggg gctgggcaaa aacctctacc acaccttgtc ggtgtatgaa
     2281 aacgtcgatt ttttcgcccg cctgttcggt cacgacaaag cggagcggga agtgcgaatt
     2341 aatgagctgc tgaccagcac ggggttagca ccgtttcgcg atcgtccggc agggaaactc
     2401 tccggcggga tgaagcaaaa acttgggctg tgctgcgcgt taatccacga cccggaactg
     2461 ttgatccttg atgagccaac aacgggggtt gacccgctct cccgcgccca gttctgggat
     2521 ctgatcgaca gtattcgcca gcggcagagc aatatgagcg tgctggtcgc caccgcctat
     2581 atggaagagg ccgaacgctt cgactggctg gtagcgatga atgccggaga agtgctggca
     2641 accggcagcg ccgaagagct gcggcagcaa acgcaaagcg ccacgctgga agaagcattt
     2701 attaatctgt taccgcaagc gcaacgccag gcgcatcagg cggtagtgat cccaccgtat
     2761 caacctgaaa acgcagagat tgccatcgaa gcgcgcgatc tgaccatgcg ttttggttcc
     2821 ttcgttgccg ttgatcacgt caatttccgc attccacgcg gggagatttt tggattcctt
     2881 ggttcgaacg gctgcggtaa atccaccacc atgaaaatgc ttaccggact gctgcccgcc
     2941 agcgaaggtg aggcgtggct atttgggcaa ccggttgatc caaaagatat cgatacccgc
     3001 cgtcgtgtgg gctatatgtc gcaggcgttt tcgctctata acgaactcac cgtgcggcaa
     3061 aacctggagt tacatgcccg tttgtttcac atcccggaag cggaaattcc cgcgcgagtg
     3121 gctgaaatga gcgagcgttt taagctcaac gacgttgaag atgttctgcc ggagtcattg
     3181 ccgctcggca ttcgccagcg gctttcgctg gcggtggcgg tgattcatcg cccggagatg
     3241 ttaatcctcg atgagcctac ttctggtgtc gatccggtgg cgagggatat gttctggcag
     3301 ttgatggtcg atctctcgcg ccaggacaaa gtgactatct tcatctccac ccactttatg
     3361 aacgaagcgg aacgttgcga ccgcatctca ctgatgcacg ccggaaaagt gcttgccagc
     3421 ggtacaccgc aggaactggt tgagaaacgc ggagccgcca gtctggaaga ggcatttatc
     3481 gcctatttgc aggaagcggc agggcagagc aacgaagccg aagcgccgcc cgtggtacac
     3541 gacaccaccc acgcgccgcg tcagggattt agcctgcgcc gtctgtttag ctacagccgc
     3601 cgcgaagcgc tggaactgcg acgcgatcca gtacgttcga cgctggcgct gatgggaacg
     3661 gtgatcctga tgctgataat gggttacggc atcagtatgg atgtggaaaa cctgcgcttt
     3721 gcggtgctcg accgcgatca gaccgtcagt agccaggcgt ggacactcaa cctctccggt
     3781 tcccgttact ttatcgaaca gccgccgctc accagttatg acgagcttga tcgtcggatg
     3841 agtgcgggcg atatcacggt ggcgattgag atcccgccca atttcgggcg cgatatcgcg
     3901 cgtggtacgc ctgtggaact cggcgtctgg atcgacggag cgatgcccag tcgtgccgaa
     3961 acggtaaaag gttacgtgca ggcaatgcac cagagttggc ttcaggatgt ggcgagccga
     4021 caatcgacac ccgccagcca aagcgggctg atgaacattg agacgcgtta tcgctataac
     4081 ccggacgtaa aaagcctgcc agcaattgtt ccggcggtga tcccgcttct gctgatgatg
     4141 atcccgtcaa tgctaagcac ccttagcgtg gtgcgggaaa aagagcttgg gtcgattatc
     4201 aacctttacg tgacccccac cacgcgtagt gaatttttgc ttggtaaaca gttgccatac
     4261 atcgcgctgg ggatgctgaa ctttttcctg ctctgcggcc tgtcggtgtt tgtgtttggc
     4321 gtaccgcata aaggcagttt cctgacgctc accctggcgg cgctgctgta tatcatcatt
     4381 gccaccggaa tggggctgct gatctccacc tttatgaaaa gccagattgc cgccattttc
     4441 ggaacggcga ttatcacgtt gatcccggcg acacagtttt ccgggatgat cgatccggta
     4501 gcttcgctgg aagggcctgg acgttggatc ggcgaggttt acccgaccag tcattttctg
     4561 actatcgccc gcgggacgtt ctcgaaagcg ctggatctga ctgatatgtg gcaacttttt
     4621 atcccgttgc tgatagccat cccgctggtg atgggcttaa gtatcctgct gctgaaaaaa
     4681 caggagggat gatgcgccat ttacgcaata tttttaatct gggtatcaaa gagttgcgca
     4741 gtctgctcgg tgataaagcg atgctgacgc tgattgtctt ctcgtttacg gtgtcggtgt
     4801 attcgtcagc gaccgttacg ccaggatcgt tgaacctcgc gccgatcgcc attgccgata
     4861 tggatcaatc gcagttatcg aaccggatcg ttaacagctt ctatcgtccg tggtttttgc
     4921 caccggagat gatcaccgcc gatgagatgg atgccggact ggacgccgga cgctatacct
     4981 tcgcgataaa tattccgcct aattttcagc gtgatgtcct cgccggacgc cagccggata
     5041 ttcaggtgaa cgtcgatgcc acgcgcatga gccaggcatt taccggcaat gggtatatcc
     5101 agaatattat caacggtgaa gtgaacagct ttgtcgcgcg ctaccgtgat aacagcgaac
     5161 cgttggtatc gctggaaacc cggatgcgct ttaacccgaa ccttgatccc gcgtggtttg
     5221 gcggggtgat ggcgatcatc aacaacatta ccatgctggc gattgtattg accggatcgg
     5281 cgctgatccg cgagcgtgaa cacggcacgg tggaacactt actggtgatg ccgataacgc
     5341 cgtttgagat catgatggcg aagatctggt cgatggggct ggtggtgctg gtggtatcgg
     5401 gattatcgct ggtgctgatg gtgaaaggtg tactgggcgt accgattgaa ggctcgatcc
     5461 cgctgtttat gctgggcgtg gcgctcagtc tgtttgccac cacgtcaatc ggcattttta
     5521 tggggacgat agcgcgttca atgccgcaac tggggctgct ggtgattctg gtgctgctgc
     5581 cgctgcaaat gctttccggt ggatccacgc cgcgcgaaag tatgccgcag atggtgcagg
     5641 acattatgct gaccatgccg acgacacact ttgttagcct cgcgcaggcc attctctacc
     5701 ggggtgccgg attcgaaatc gtctggccgc agttcctgac gctgatggca attggcggcg
     5761 cgtttttcac cattgcgctg ctgcgattca ggaagacgat tgggacaatg gcgtaaggaa
     5821 gggaaacttg acttcatact gtgatgtcat actattgatg tcataaagtg aagtcataag
     5881 gacatgccat gagaaccaag gtacaggctt tacggaagaa acaaaaaaat acattggatc
     5941 aaatatttaa aactcctgtt cctcaaggga tcaagtggtc tgatatagag tcactggtta
     6001 aagcattagg cggagaaatt aaggaaggaa gaggttcgcg ttgtaagttc atactaaata
     6061 tgagcgttgc gtgtttccat cggcctcatc cgtcgccaga taccgataaa ggcgctgtag
     6121 aaagcgtgcg tgactggtta ctaagtatag gggtaaaacc atgatcaaac tgaagacgcc
     6181 aaactctatg gagattgccg ggcaaccagc tgttattacc tacgtgccag aactgaatgc
     6241 atttcgtggc aaattcctgg gcttgtcagg ttattgtgat tttgtctctg acagcattca
     6301 ggggctgcaa aaagaagggg aactttccct gctagagtat cttgaggatt gtaaagctgc
     6361 gggtattgag ccgtatgcgc gtacagaaaa gattaagacc tttacgttac gctatccaga
     6421 atcgctgagt gaacgcctta ataatgcggc tgcgcagcaa caagtctctg ttaataccta
     6481 tattattgaa acactgaatg agcgtttgaa tcatttataa attttagcgg cgtacattac
     6541 gcagaatatc tgcctttaca ggatctatct atgcaactga acttaatttt ataaattaag
     6601 ttcagttaat atcttttctt tcctcgtttc agaaataact caatcttcct tcggcaagca
     6661 ctgcaaatgc ccatgccgca cgccgcgctg ggcgataacg tcatcggcaa aatgctgcac
     6721 gtcgcccata tcacctttca gcacggcgat ttccagacag tcgtcgtgat tgatatgcac
     6781 atgcagcgtg gcgacggaga gatcgtggtg atggtgctgg gtggagacaa tgcggctggc
     6841 taagtcgcgt ttttcgtgtt catacacata ggagagcacc gcgaaacctt gcgtgccgtg
     6901 ctgctgagtg gcctcctggg ccagggcgct acgcagaata tcgcggatag cttcggaacg
     6961 gttgttataa ccacgacgct ggctcaggct gtccagcgtc tccagtaaat cgtcatcaag
     7021 cgtgatggtg actcgttgca tttgcgttaa accttttctg tggcgcgacg gcgcacgggg
     7081 aatgcgggta ataccgcgtt ttgtagcaca cgtccggcgt cagaggaaaa ggttaattta
     7141 tctcccaccg cctgggtttc gacgatttgt ccgttgtcca tcaccatcac ccgctggcaa
     7201 aagcgttcta caagacgtaa gtcgtgggtg atgaacaggc aggcggtgcc aaactgttgt
     7261 tgcagctttt tcagcaggcg aatgacgccc gcctgtaaca cgagatcaag gttagaaacg
     7321 gtttcatcca gaatcagcaa tttcggttcg accgccagcg cgcgtgccag gcagacgcgc
     7381 tggagctggc cgccgcttaa ctgcggtggg cgtttgtcga gaacgctgtc atcgagatcg
     7441 accgccttca gcatttcgct ggcgcgcgcc agttgttcgg attttttcag tgatagcagg
     7501 tggcgcatcg gttcacgcag gatctcgcgc acggttttgc gcggattcac ggcactgatg
     7561 gagtcctgaa acaccatctg aatatcacgg cggaaggctt tacgttgggc gcgattaagt
     7621 ttcgccagcg attcgccgcg ccagctaata ttcccttgtg agggagactc taagcctacc
     7681 agcagccgcg cgagggtgct ttttccgcag ccgctgcgtc ccagcagggc gacggtttcg
     7741 ccgcttttca gggtcaggga aacgttattc agcaccgcct gatgttgatg ttttccgcta
     7801 aatccaccgt gggcatagtg atgggaaagg ccgctgacgt taagtaaggt catgatgcca
     7861 gctccatacc gtagagggcg agatgagcgg aaaccaggct gcgcgtcacc gtatgtttgg
     7921 gggcgttaaa cagcgtttct acatcgccct gttcgacaat tttaccttgt gacatcaccg
     7981 ccacgtcatc cgccagacgc gccacaacgc ccatatcatg ggtgaccagc agcattcccg
     8041 gcgcttgttt ttgcataatg ctttccagca gatcgaggat gcgcgcctgt gctaccacgt
     8101 cgaggtcggt ggtcggttca tcggcgatga taaacggtga ttcacacagc accgccatcg
     8161 caatcatcat gcgctgcaac atgccgccgc tcatctcgaa cgggtacagc ttcagcacgc
     8221 gcgcggcgtt ttccagcccc accgcttcta tggcagcggt aagcgtagcg tcatcggcgg
     8281 gtttccctaa cgccaggcag gtttcccgcg cgtgggtgtg catggtgtgt agcggattaa
     8341 aggcgctgcg cgggttctgc atgatggtgg caattttgat gccgcgcagg gcgcagggat
     8401 aaaccgcttt gccatcggct aaaatttccc ccgccgtctg gcgaacgcca gcgggcaaaa
     8461 tgcccagcgt cgcggcgcag gttaatgatt tcccgctgcc gctaccgccg actaacgcca
     8521 gcacgcgccc gcgttgcagg gttaacgata caccgtgtac cagcggctgc gcggcctgta
     8581 gcgcgatatt acgtagttca atctgttgcg gcattagtgt gcgtgctccg tcaccagatg
     8641 agggtccaga tgatcgcgca gtgcgtcacc caccaggtta aaggccatca cgctgataaa
     8701 cagcgccagc cccggccaga acatttgcag cggctgggtc cagatatact ggcgcgcgtc
     8761 gttaatcatc acgccccatt cggcggtcgg cgcggtcaca ccgaggccaa ggaaagacat
     8821 ccccgagacg tgcagcatca tatggccgat atccagcgtt gccagcacca gcagcgaagg
     8881 gatcaccgcg cctgccagat gatcgacaaa cactcgcaca tggcccgcac cggaaagccg
     8941 tgacgccagc acaaactcgc gttggcgtag tgaaatcacc aggctgcgca ccatgcgtgc
     9001 ataccacgcc cagtgcgaca gggcgatggc gataattacg ttggtcagcc cggtgccgag
     9061 cacgccaacc ataaagaacg acagaatcga ggtcgggaag gtcataaaca tatcggcgac
     9121 gcgcatggtg gcctgatcaa cgcgcccgcc aatcaacccg gcgctgccgc caataaccag
     9181 ccctaatgtc agcaccagca gcaggcaggc cattaccgaa ccgagcgaca cgcgggtcgc
     9241 tgccatcagc cgcgagaaaa tatcgcgacc taagtgatcg gtgcccagcc agtgctgcgc
     9301 atccggcgaa agcaggtgcg acggcaaatc aatcgcctgg gggtcatacg gcagccacca
     9361 ctggctggtg agcgcaatca gcgccagcag ggcgataatg atcagcgcca ggcgtaccga
     9421 ccagcgggaa gagaggaaaa agttcacgcg tgcgctcctt catgacgacg aatgcgcggg
     9481 tccagcgcgg cgttgagcaa atcgacaatc aaattacaga ccacaaaaac caccgccatc
     9541 atcagcgtaa agcactgaat caccggatag tcacggttaa aaatcgccga caccgcatag
     9601 cgcccgacgc caggccaggc aaagatgttt tcgataatca tcgtcccgcc aatcaattcg
     9661 ccaatgtgca tccccacggc ggtgatcatc ggcagcgagg cattgcgcag aatatggcgg
     9721 cgttcggtct gtttgtcatt caggccgcgt agccgcgccc aggtgacgtg acgctgaccg
     9781 gcgacgtcca gcatactggc gcgcagcagt cgcgcgttaa tcgccagcga cataaaggcg
     9841 atggaaaccg caggcaaaat gatgtgctgc cagccgccgt agcccatcgc gggtagccat
     9901 tgcagataca ccgaaaacgc catcactagc aaaaatgcca gccagaagtt gggcatcgac
     9961 acgccaagaa acgcgataaa gcgcacggcg aaatccggca aacgatcgcg gtggcgcgcc
    10021 gcccagatac cgagcggtac ggaagtgagc agaattaata ccagcgccgc acctgcaagt
    10081 tccagcgtgg cgggcaggaa gttcagcata tcgtccagca ccgggcgttg gctggcgaat
    10141 gagataccaa agtcaagatg cagcgccttc cacaaccagg tgccgtactg gacgtacagc
    10201 ggctgatcca gtccgagcat ggtgcgggta gaggccagca tctccggcgt cggcggcagg
    10261 ttagacagac gcaaataatc gagcgccggg tcgccggtac cgaggcgcag catcagaaaa
    10321 atgatcaccg aggcggcgag caccatcggg atcagcagca gaaagcggcg taatacgtaa
    10381 cgcaacatta aggtttcacc ggtttaatct gttcgaacgg aatttcggtg gcgatcggcg
    10441 cgtaggggat gttacccagc tccggttttg ataccaccat cattgagatg taactgatag
    10501 gcagataaac cgcctcgtca tgcagacggg tcagaatgtc gcgatacagt gcctgacgtt
    10561 gcgtttcgtc atgggtcgcc agcacttcgc cgatctcttt atcaatcagc ggtttgtcgg
    10621 ctaatccttg ctgtgcctgg aagtcagcgt gcgacggtac gcgcattgaa ctcaggaagg
    10681 cgtgtggatc gtatggcgcg ccccaggtgc ggtggaaaat catgccaaaa cgaccgtcgc
    10741 gctgacgagc atagatactg ctctcttctt cgccaatcag cgagacatct gcgccaatct
    10801 ggcgcatatc agcctgaatg atttccgcca tcgatttgct taacgcatcg gtgccgatga
    10861 acgacagttc aatgcgcagc ggctgaccat ttttctcgcg gatgtctttg cccgcaggca
    10921 gcgtccaacc ggctttttcc agcaacgctt tcgctttctg cggatcgtac tggcgcggtt
    10981 tcaggccgag gttggcgtag ggcacagaag gggcaaacag ggtgtcggcg acctgctggg
    11041 tgccatacaa cgcgttatca atcagcgatt ttttgtttac cgcgtaatta agagcttcac
    11101 gtactgccag ctcattggtg ggggttttgg cggtattaag cgccagcatc acggtttcga
    11161 tcggctgtga cagttgggta tggtaagccg ggttctggct aaagcgggcg aaggtatcga
    11221 gcggtaataa cccttcgttt ccgtacagca ggtcgatatc gccagtttca aacgccaccg
    11281 cgcgggtagt cgggtccggg atgacgttaa aggtgatctt tttaatcgct ggcttttcgc
    11341 cccagtagtt ttcgttacgg acgaagacat cgtactgatt cagtttcgat tcctgcaaaa
    11401 tccacggtcc ggtgccaatc ggcgctttaa ttccgttcat ggtttcatgg tttttaaact
    11461 gcgagggagc gataaaacgg aaaggacggg gcagggccag ttcttgcagg aaaggatagt
    11521 aggcgctttt cagggtaatt tgcagctctg ttttactgag tgctttaaca tcaacaatct
    11581 ggtttgccag ctccagccag gcgtgacgtt gacggttatc gagcactgcg cggaagtttt
    11641 ctgccgccgc ctcggcatcg aacggttcac cgttggataa tttcacgtca tcacgcaggg
    11701 tgaaggtcca ggttttacca tcttctgaat gagtccagct ttttgccagc cacgggatca
    11761 ccgaaccgtc tgcctgatat ttcaccaatg gttcataaac catgctctgg gcgaacatct
    11821 ggttaggcgt gtaaaggtgc gggtttagtg gcccgacatt caccggccag gcggtagtga
    11881 tttcatctgg tgcagcggca tggacgataa aagacgcaca agccagcagc gcaaatagag
    11941 tgcggcggag tgtggagagc atgataaccc caatggatta aaatagatgg cggaaataag
    12001 tatgacgatt ttaagtattc gtcatactga ttacctgttc tggatcaata gatgggcagt
    12061 cgggtgggcg gatcagttaa ctgaatcgat ccattgcaca ctgtcggcgg tgagcgtaaa
    12121 gggggtgggg gtgcagatcg caaggcttaa attttctagc tgacaatggc tgactgacag
    12181 cgaggagtga taggtgctat ctacgctgac gatttgccag gcgctgccgc cacgctgttt
    12241 aacgatggct tctttgcgcg tccagatgcg ccagaacatt tccagttgct gatcaggatg
    12301 cacggcgtcc atttcagcgt gttccccgag gctgaacacg gcgttcgcca gccagcgcca
    12361 gttggcgcgc gggcgaatca cttcgatatc gcagccgact tcgccttcat cgctcaacag
    12421 cagagcgata tcgtcaccgc tatggcttaa gttgaaccat agcggcgttt ccggcgcgaa
    12481 tgcaggtttg ccttgttcgc catagatgat ctccggtagc ggggaaagcg tgtgcgaaag
    12541 caatgcacgc cccgccagcc agcgttcgcg tcgtggacct tgcggtgctt gctcgcgtaa
    12601 acccggtggc agtggagctg cgcttaaggt cgaaactttc cccagaacta tccgatacat
    12661 atcagggcca acgtttaatg gaaaatgaaa gtgcgtatcg tatcacttgt cgcctcatcc
    12721 cggtaaccga cttttcggtc tgcctagccc cagtaaaatc gccagtttgc taccgccttt
    12781 ggtggtttcc atccagattt tacacacgct ggttaatggc accgaaagta gcatccctac
    12841 cgggccgagc agccatcccc aaattaacaa tgaaagaaat accaccatgg tggacatccc
    12901 caggcgatgg cccatcatcc gtggtcgatg accatatgga ccactaaaaa caatgcgccg
    12961 accagaatac atgcgtaaac accattaaac agcagcacct gaatcattgg cggtacggcg
    13021 gaaattaccg cgccgatatt gggcacgtag ttgagcaaaa acgccagtac tgcccacatc
    13081 agcgcaaact gtacgcccat tagcgccagc cccagccaga cgatgacacc tgtccataaa
    13141 ctgagtagcg tctttaacgc cagataatgc gaaacgcctt taagtgcgcg gtgtaatcct
    13201 gcgatgtgaa tctgtgggtt attcagcgca aaacgcattt tgtaagggac gtggcgcact
    13261 tcaaacagca taaaaactac ggtcatcacc agcaaaagca cgctcgccat tgccccggaa
    13321 agcccgatca ttagcgctgt ggtgaaggta accacttttt ccgagtccat ccgctgcagc
    13381 attcgctccg gcgacatatg caaattaaga aaaggcaaca tctcctgcaa tttaaaaagt
    13441 ttgcgcgtca gctccttatt aaacttcggc agcatagaga taaattcgtt aaacgatgcc
    13501 gccagtacgc cgactagcgc ggttagtgcg atcagcatca ccaccactac aatcgtaatg
    13561 gtaacggggc gttgtactcc ccgacgaata aaccaggtaa cgagcgggtt gaggacgatg
    13621 gcaaaaaaca gcgccagtaa cagctgcaca atgatatctg ccgctgcgtg aatgcccgcg
    13681 aggatcacta ccagcgaggc caacttgagc agaatgtgca tgcccgtttt atcgggttga
    13741 ggggtttcca ttggggcttc cttgtgactt tttgtgttaa gtgtagcggg agtcacgtca
    13801 gcaatattct gattcttact gctgattttc acgtcagcac gtggtaaaaa tgaaacactg
    13861 ttgtaaaaat gtggtgatcc tcatgcccga acccgtagcc gaacccgcgc taaacggatt
    13921 gcgcctgaat ttgcgcattg tctccattgt catgtttaac ttcgccagct acctcaccat
    13981 cgggttgccg ctcgctgtat taccgggcta tgtccatgat gtgatgggct ttagcgcttt
    14041 ctgggcagga ttggttatca gcctgcaata tttcgccacc ttgctgagcc gtcctcatgc
    14101 cggacgttac gccgatttgc tgggccccaa aaagattgtc gtcttcggtt tatgcggctg
    14161 ctttttgagc ggtctgggat atctgacggc aggattaacc gccagtctgc ccgtcatcag
    14221 cctgttatta ctttgcctgg gacgcgtgat ccttgggatt gggcaaagtt ttgccggaac
    14281 gggatcgacc ctgtggggcg ttggcgtggt tggctcgctg catatcgggc gagtgatttc
    14341 gtggaacggc attgtcactt acggggcgat ggcgatgggt gcgccgttag gcgtcgtgtt
    14401 ttatcactgg ggcggcttgc aggcgttagc gttaatcatt atgggcgtgg cgctggtggc
    14461 cattttgttg gcgatcccgc gtccgacggt aaaagccagt aaaggcaaac cgctgccgtt
    14521 tcgcgcggtg cttgggcgcg tctggctgta cggtatggca ctggcactgg cttctgccgg
    14581 atttggcgtc atcgccacct ttatcacgct gttttatgac gctaaaagtt gggacggtgc
    14641 ggctttcgcg ctgacgctgt ttagctgtgc gtttgtcggt acgcgtttgt tattccctaa
    14701 cggcattaac cgtatcggcg gcttaaacgt agcgatgatt tgctttagcg ttgagataat
    14761 cggcctgcta ctggttggcg tggcgactat gccgtggatg gcgaaaatcg gcgtcttact
    14821 ggcgggggca gggttttcgc tggtgttccc ggcattaggt gtagtggcgg taaaagcggt
    14881 tccgcagcaa aatcaggggg cggcgctggc aacttacacc gtatttatgg atttatcgct
    14941 tggcgtgacc ggaccactgg ctgggctggt gatgagctgg gcgggcgtac cggtgattta
    15001 tctggcggcg gcgggactgg tcgcaatcgc gttattactg acgtggcgat taaaaaaacg
    15061 gcctccggaa cacgtccctg aggccgcctc atcatcttaa aacttactga ataaccagcg
    15121 tattaatgat gttttctgcg gtggtctgcg ctttttgctg atcgtcagcg ggcagcgtaa
    15181 tttgcatggt cagcagttga ttacccacgt tacccagaat aacggaagag tacgccgtct
    15241 ggcctttcgc ggagataata ctgtctaact gctgcatttt gtgacctttc agctcaatgg
    15301 ctttattggt taccacttgc agctgcggat cgcggctacg ttgctgatct tccagacgct
    15361 tcgccagcac cgccagatct tctttcggat catcgcccat gatgacgatg actgctttct
    15421 gcccggtggc gtcggaccag acatgcatgt tattggcctg cgttcccagc ttaccgctct
    15481 ggtcggtcat atccgctggc agcgagaaac ttaacttgcc atcaagcagg ttgacgggat
    15541 tcccggtagt gttattttcc gcgaccgaac cctgcgccgt agcgttagtg tctttatcat
    15601 cacaggccgc aagccccata accagcaggc caattccgac atatttaacc agattgcgca
    15661 ttgacttctt cctttcgata aacggccata acggctcatt catccatctt atcacaactc
    15721 tgataacgaa cctttaactc gcctgcaaag cgttgatttc ggatttatct gccagtcttt
    15781 tcaacagcat attgagtaat acgccataca ttggcaggaa gaaaacgata ctgattaaca
    15841 ctttgaaaca gtaatcgacc agcgcgattt ccatccagtg ttcagccata aaggcatccg
    15901 ggctacgcca gaaggcaatg aagaaaaagg ccagcgtgtc gctgacgtta ccgaacagtg
    15961 tggacgctgt cggtgccagc caccagcggc gactctgacg caggcggtta aaaacgtgca
    16021 cgtcgaggat ctgtcccagc gcgtaggcca tgaaactggc ggtggcgata cgggcgacaa
    16081 acaggttgaa gtgggcgagt gcgccgaatc cctgccagga acccatatag aatagcgacg
    16141 agatgacgta ggagatcagc aacgccggga tcatcaccgc aaagatgata cgacgggcca
    16201 gcggtgcgcc aaaaatacgc acggtcaggt cggtagcaag gaaaataaac gggaagctaa
    16261 acgcgcccca ggtggtatgg aaacccaaaa tggagacggg aagctgaacc agatagttac
    16321 tggaggtgat caccagcaga tgaaataacg ataaccagaa caacgcctta tagcgttgag
    16381 tttgcgagaa aacgttcata ttgtaccttt ttgattaacc attggggtga gggaacccaa
    16441 tacgtacgac acgtctgtta ccttaacata ataacgtgct tacccttttt tcgagccgcc
    16501 gcatgatact gctttacgtt gacaatgcaa tggttaattt tcacgcaatc gttaacctgg
    16561 tttgcttacg gactcacagg gcgtaaacta gcgccgtttt tttatgtgat gagaagaaaa
    16621 tgaccgatct cttttccagc cctgaccaca cactcgacgc acttggcctg cgctgcccgg
    16681 aacccgtaat gatggtgcgc aaaactgtgc gcaatatgca gcccggcgaa acgttgctga
    16741 ttatcgccga cgatccggcc actacccgcg atattcctgg gttttgtacc tttatggaac
    16801 acgaactggt tgcgaaagag acggatggac tgccttatcg ttatttgatt cgtaaaggcg
    16861 gttgatgggg gctgattggc ttcgatgccg ccttttcccc tcaccctaac cctctcccca
    16921 gaggggcgag gggaccgatc gcgctcaatg ttgcgatcgg tttgccttat ctcctgcgca
    16981 acaatcttaa cgcattcgct gtcaccagca ccgtcgcacc cgtatctgcc agcaccgcca
    17041 gccatagtcc ggtcatccct aaaagcgtgg tgacgaggaa gatccctttc agtcccagcg
    17101 caatggtgat gttctggcgg atattggcgt gagtggcgcg tgccagttca atcatttgca
    17161 ccaggccgcg caggtggtta tgggttaatg ctgcatcagc ggtttccagc gccacgtctg
    17221 tgccactgcc cattgcaatc ccaatggcgg cagctttcat cgctggcgcg tcgttaatac
    17281 cgtcaccgac catcgccagc ggcgcatgtt gattcagctc ggtcaccgct ttgactttat
    17341 attccggcaa caggcccgct ttaaactcca gccccagctc cccggcaatt gccgccgctg
    17401 cgcgtggatt atcgccggtg aggatcaccc ctttgacgcc cagcgcgttc agttcactga
    17461 tggcagttgc agcatcggcg cgcagggtat cctgcaatgc aatgataccc agcacgtcat
    17521 cattacgtac taccagcaca accgtttgcc cggcgctttc cagttcatta atcaaaccag
    17581 caaatgcatc agcgggatgt ttcccggcag cgcatatcaa cacgcgctca ccgttaacct
    17641 gcgcttcaat gccagacccg accagcgccc gctgtgattc ggcggcggga atggcgagtg
    17701 cagcaacctg tgcttcgcgc acgatggcct gcgccagtgg atgcgtcgcg ctttgctcga
    17761 ccgccgccgc cagtgtcagc agttcagatt cactaatacc cgttgccgga tgaatcgcgg
    17821 taacgcgcgg tttaccgacg gtcagcgtac cggttttatc aaacgccacc tgagtaacac
    17881 gacctagttg ttccagcgcc gctccgcctt taatcaacgc cccacgacgc gctgctgccg
    17941 ccagcccgga ggtaatcgcc gcgggcgtgg agataactaa cgcacacggg cagccaatca
    18001 gcagcagcgt caggccttta taaatccact cctgccagct ggcggcaaac agcagcggtg
    18061 gcaccagcgt caccagcagg gcgacggcca taatcgctgg cgtataaata cggctgaaac
    18121 ggtcgataaa ccgctcaatg ggggcgcggc gctcttcggc ttcttcgatc agtttcagaa
    18181 tccggtcaat ggcgctggca cccggttctg acagcacttc cagcgttacc agacggtcta
    18241 cgctggtggc tcctgcagga actttatcgc ccgtcgcgcg ctccaccgga atggattctc
    18301 cggtcagggc gctttcatca aaactggcaa acggtgagag cagtttaccg tcggcaggca
    18361 aacgcccacc tgcggcgact tcaatcacat cgccaggccg caggctgtta atcgccacct
    18421 cttcccgctc accgttacgc aggcgcgtgg cggtttctgg tttcagcgcc attaacgcgc
    18481 taacgccctg acgcgcgcgg ctggcggccc agccttccag tcgttcacca atcaaaaaca
    18541 gcagcaacac catcgcagct tcagccgttg cgccaataaa cagtgcacca atagcggcta
    18601 cgctcattaa ggtttcaatg gcgaagtagc tgccggattt gatcagccgt aatgcctgat
    18661 gagcaatcgg gtacagccca accagcgtgg tcgcgataaa cgccagttgc ccgaacggat
    18721 gattgaactg ctccagaccc cagctgattg ccatcatcac gattagtgta atcagcggca
    18781 gattctcttt caggcgcgat gcttgtggct cgtcggcggc ctgctcatcg cgcagggaat
    18841 agcccgcttt ttgcaccgca gattcaactt gtgcacgaat gtcattgtcg gcatcaacca
    18901 ccagtttttc ggtggcgaac aacacctgca cctgattcac acctgcaagc tggcgcacgg
    18961 cattttctac cttgcgcgca caggcggcgc agtccatgcc actgactttc cagctatagc
    19021 gggtgccgga gacgttttca gagagcgttg gcgagctgga gcatgcgccg tcgcagcaac
    19081 agtcgttggc gttctgtacc gtggttagcg gtttgaacgc agcaaattga ggggctttct
    19141 tgccgtgatt gtcaggtgtc gacatggcat actccggtta agttttttct cattaaccga
    19201 aggatacact ctggagtcga ctccagagtc aagttttatc agaaatacag tgagcggacg
    19261 atgaggaagt gaccggcaaa gtagcaggcc gcagcaatcg cgttatccgc acggaagcgt
    19321 cggcgatagt ggctccccag ccagacaaag ttactgataa acagcaacga agcgccaaca
    19381 aacgcagaga gcgccggagc ggtcggacgg aagaaccaca gttcacctgc cagccacacc
    19441 atcaccagcg tcatgccgat aaacgtgcag ataggccaac gatactcttc cagccgcgtc
    19501 cagataatcg ccagtaacag cgcacccaga accagcaaca ccagtggcag aggccagaag
    19561 aaagagagcg tcatctgact ggcgaaatag atggtgtaca gcaggtgcga gaggaaaaac
    19621 gcgccgatgg cgtacatcag acgttgacgt ggcaacaggg ttagcgcatc gcccagcagt
    19681 gaggcgcaca gccctgccag caccagatag ctaatggcgt cgaacatcgg cgcttgccag
    19741 gccagtaaca gcaggagaag aagggttaac ggtttaaaca cccagcgttg ccaggttggc
    19801 ccacgatacg atgcatccac agatagccat gcggaaagac agacagcgat aaacgaccaa
    19861 agcatcttag ttccttgaat tcgttatttt ttcgtaagcg gaatgcctac ggtcttgctt
    19921 ccagtttagt ggcgagggtg gacggatgac aacaccataa ctagcagggt atgcttattt
    19981 ccgcattttc cgatgaggga taaagatgag caaaccacct cttttcttta ttgttatcat
    20041 tggcttaatt gtcgtcgccg catcgtttcg ttttatgcaa cagcgacggg aaaaagctga
    20101 taatgatatg gctccgctcc agcaaaagct ggtggtggtg agcaacaagc gggaaaaacc
    20161 gattaacgat cgccgttcgc gccagcagga agtgactccg gcaggcacca gtatgcgcta
    20221 tgaggcaagc ttcaaaccgc aaagcggagg aatggagcag acgtttcgcc tcgacgccca
    20281 gcagtaccat gccctgacag tgggcgataa aggtacgctg agctataaag gaacgcgctt
    20341 tgttagcttt gttggcgaac aataaggtta ttttttaact ttaaatttct tctgccagac
    20401 cagcagttca aacacgccga aaaggaaaat ccgcaccttt tcggcggtgg tcatctgcgg
    20461 gccatctttc ggtaaagtgg atttcaatag cgccagctgc ataccatgca tcagcacggt
    20521 aaaaatcagc gccacgttaa cgaaaatatt cagcgggcgc gggaagggat gcaccagatt
    20581 gaggattaaa aatccccaaa cgcagagcat taacaaacga ccaatattaa tcagcatcac
    20641 tttctccttg tgcttcgcgt tgatacagcc gataagccac ctgacccgcc actttttccc
    20701 gatgtaatga ccagtttgcc ggaacagtgg gcagaccgtt ttcgacttcg ctttcgacat
    20761 aaatcagggc ttcgtcagcc agccagccgt tatcttccag taaatttatc gtctcttcta
    20821 acaagccacg gcggaacggt ggatcgacaa acacgatatt atgcggtgta cctttttgcg
    20881 ccaggaatga catcgcgttg ctgttcacca cgcgtgcatt gcctgctttt agtgtcgcca
    20941 gattcttaat taactgctga gaaaccgcgc gatccatctc aatcaacgtg gcccccgcag
    21001 cgtagcgcga taacgcttcc agtcccagcg cgccgctccc ggcgaagcaa tccagacatt
    21061 gggcgtcaac aatgaccgga gccagccagt taaacaatgt ttcgcgtacg cggtcggtgg
    21121 tggggcgcag acctgggcta tcaggaaccg ggagtttacg gcctcgccac tgcccgccaa
    21181 taatgcggat ttggccgctg ccggaatgat tcggtttttt catgataaat tgctcaatcc
    21241 gccagataac acatacttgc aggcggtgat gtgaattaag ttaagtgata gactatttca
    21301 tcattttttt agctgctatg tacatagcgt taacgctgtg ccatgaagca acagcgagga
    21361 gtgtagtcgc aaatggcgaa agaaaaaaaa cgtggctttt tttcctggct gggctttggt
    21421 caaaaagagc agaccccgga aaaagagaca gaagttcaga atgaacaacc ggttgtagaa
    21481 gaaatcgttc aggcgcaaga gcctgcgaag gcttctgaac aagccgtcga agagcaaccg
    21541 caggcgcata ctgaagccga ggcggaaact tttgctgccg acgttgtgga agtcactgaa
    21601 caggttgctg aaagtgaaaa agcgcagcct gaagcggaag tcgttgcaca gccggaaccg
    21661 gtcgtagaag aaacgccgga gccagtggct atcgaacgtg aagagctgcc gttgccggaa
    21721 gacgtcaacg ccgaagaggt ttcgccagaa gagtggcagg ctgaagcgga aaccgtagag
    21781 attgtcgaag cggcggaaga agaagcggct aaagaagaaa tcaccgacga agagctggaa
    21841 gcacaggcgc tggctgccga agcggcagaa gaggcggtga tggtggttcc tccggtagaa
    21901 gagcagccgg tggaagaaat cgctcaggag caggaaaaac cgaccaaaga aggtttcttc
    21961 gcgcgcctga aacgcagcct gttaaaaacc aaagaaaatc tcggttccgg atttatcagc
    22021 ctgttccgcg gtaaaaaaat cgacgatgat ctgtttgagg agctggaaga acagctgttg
    22081 atcgccgatg tgggcgtgga aaccacacgt aaaattatca ccaatctgac ggaaggcgca
    22141 tcccgcaagc agcttcgtga tgccgaggcg ctctatggcc tgctgaaaga agagatgggc
    22201 gagattctgg cgaaagtcga tgagccgctg aatgttgaag gtaaaacgcc gttcgtgatc
    22261 ctgatggtgg gcgtcaacgg tgtgggtaaa actacgacga ttggtaagct ggcacgtcag
    22321 tttgagctgc agggtaaatc ggtgatgctg gcggcgggcg atactttccg tgcagcagcg
    22381 gttgaacagc ttcaggtctg gggtcagcgc aacaatattc cggtgattgc ccagcatacc
    22441 ggtgcggatt ccgcctctgt tatcttcgac gccattcagg cagctaaagc gcgtaatatc
    22501 gacgtcctga ttgccgatac cgccggacgc ctgcagaaca aatcgcacct gatggaagag
    22561 ttgaagaaaa tcgtccgcgt gatgaagaaa ctcgacgttg aagcgccgca tgaagttatg
    22621 ctgactattg atgccagtac cgggcagaat gcggtaagcc aggccaaact gttccacgaa
    22681 gccgttggct taaccggcat cacgctaacg aaactggacg gcacggcgaa aggcggggta
    22741 attttctcgg tggctgacca gtttggtatc cctatccgct acattggtgt cggcgaacgt
    22801 attgaggatt tgcgtccgtt taaggcggac gactttatag aggcactttt tgcccgagag
    22861 gattaacaat gattcgcttt gaacatgtca gcaaggctta tctcggtggg agacaggcgc
    22921 tgcagggcgt tacgttccat atgcagccgg gtgagatggc gtttctgacc ggtcattccg
    22981 gcgcagggaa aagtaccctc ctgaagctga tctgtgggat tgagcggccc agcgccggga
    23041 aaatctggtt tagcggccat gacatcacgc gtctgaaaaa ccgtgaagtt ccgtttctgc
    23101 gccgccagat tggcatgatt ttccaggatc accatctact gatggaccgt actgtctacg
    23161 ataacgtggc gatcccgctg attatcgctg gtgccagcgg tgacgatatt cgtcgccggg
    23221 tgtcggcggc actggataaa gtcgggctac tggacaaagc gaagaacttc cctattcagc
    23281 tttccggcgg tgaacaacag cgtgttggca ttgcccgcgc ggtggtgaat aagcccgcgg
    23341 tactgctggc ggacgaaccg accggtaacc tggacgacgc gttgtcggaa gggattctac
    23401 gtctgtttga agagtttaac cgcgttgggg taaccgtatt gatggcaacg cacgacatca
    23461 acctgatttc acggcgttcc tatcgcatgc tcaccctgag cgatggtcac ttgcatggag
    23521 gcgtgggcca tgaataagcg cgatgcaatc aatcatattc ggcagtttgg tgggcgtctg
    23581 gatcgcttcc gtaaatcggt cggcggctca ggcgacggcg gtcgtaacgc accgaaacgc
    23641 gcgaaatcct cgccaaaacc ggtaaatcgc aaaaccaacg ttttcaacga acaggtgcgc
    23701 tatgccttcc acggcgcatt gcaggatctg aaaagcaaac cgttcgccac gtttttaacg
    23761 gtgatggtta tcgccatctc cctgacgctg cccagcgtct gttatatggt gtacaaaaac
    23821 gttaatcagg cggcgacgca gtattatccg tcaccgcaaa tcactgttta cttgcaaaaa
    23881 acgctggacg atgacgccgc tgcgggcgtg gtggcacagt tgcaggccga gcaaggcgtg
    23941 gagaaagtga actatctttc tcgtgaagac gcactgggcg agttccgtaa ctggtctggt
    24001 tttggtggtg cgctggatat gctggaagaa aacccgcttc cggcagtggc ggtggtgatc
    24061 ccgaaactcg attttcaggg gacggaatca ctcaatacgc tgcgtgatcg tatcacgcag
    24121 attaacggca ttgacgaagt gcggatggat gacagctggt ttgcccgtct ggcggcgttg
    24181 accgggctgg tcgggcgcgt ttcggcgatg atcggcgtgt tgatggtggc ggcagtgttc
    24241 ctcgtcatcg gcaacagcgt acgtctgagc atctttgctc gccgtgactc catcaacgtg
    24301 cagaaactga ttggtgcgac ggatggtttc atccttcgcc cgttcctgta tggtggcgca
    24361 ctgctgggat tttctggcgc attgttgtca ttaattttgt cagaaattct ggtgctgcga
    24421 ttgtcatcgg cggttgcgga agtggcacag gttttcggaa cgaattttga tatcaatggt
    24481 ttatcgttcg atgaatgcct gctattgctg ctggtatgct cgatgattgg ctgggtggca
    24541 gcgtggcttg ccacggtaca acatttacgc cactttacgc ctgaataata aaagcgtgtt
    24601 atactctttc cctgcaatgg gttccgtagc agggaaagag atcccgttgt ctcttcccgg
    24661 tatttcatct ctatgtcaca ttttgtgcgt aatttattca caagcttgca ttgaacttgt
    24721 ggataaaatc acggtctgat aaaacagtga atgataacct cgttgctctt aagctctggc
    24781 acagttgttg ctaccattga agcgccagaa gatatcgatt gagaggattt gaatgactga
    24841 caaaatgcaa agtttagctt tagccccagt tggcaacctg gattcctaca tccgggcagc
    24901 taacgcgtgg ccgatgttgt cggctgacga ggagcgggcg ctggctgaaa agctgcatta
    24961 ccatggcgat ctggaagcag ctaaaacgct gatcctgtct cacctgcggt ttgttgttca
    25021 tattgctcgt aattatgcgg gctatggcct gccacaggcg gatttgattc aggaaggtaa
    25081 catcggcctg atgaaagcag tgcgccgttt caacccggaa gtgggtgtgc gcctggtctc
    25141 cttcgccgtt cactggatca aagcagagat ccacgaatac gttctgcgta actggcgtat
    25201 cgtcaaagtt gcgaccacca aagcgcagcg caaactgttc ttcaacctgc gtaaaactaa
    25261 gcagcgtcta ggctggttta accaggatga agtcgaaatg gtggcccgtg aactgggcgt
    25321 aaccagcaaa gacgttcgtg agatggaatc acgtatggcg gcacaggaca tgacctttga
    25381 tctgtcttcc gacgacgatt ccgacagcca gccgatggca ccggtgctct atctgcagga
    25441 taaatcatct aactttgccg acggcatcga agatgataac tgggaagagc aggcggcaaa
    25501 ccgtctgacc gacgcgatgc aaggtctgga cgaacgcagc caggacatca tccgcgcgcg
    25561 ctggctggac gaagacaaca agtccacgtt gcaggaactg gctgaccgtt acggtgtttc
    25621 cgctgaacgt gtacgccagc tggaaaagaa cgcgatgaaa aaactgcgcg ctgctataga
    25681 agcgtaattt ccgctattaa gcagagaacc ctggatgaga gtccggggtt tttgtttttt
    25741 gggcctctac aataatcaat tccccctccg gcaaaacgtc aatccccacg cagattgtta
    25801 ataaactgtc aaaatagcta ttccaatatc ataaaaatgg ggtatgtttt agcagagtat
    25861 gctgctaaag cacgggtagt catgcataaa acgaaataaa gtgctaaatc acaacaccac
    25921 aacacacgta ataaccagaa gaatggggat tctcaggatg aatataaagg gtaaagcgtt
    25981 actggcagga tgtatcgcgc tggcattcag caatatggct ctggcagaag atattaaagt
    26041 cgcggtcgtg ggcgcaatgt ccggtccggt tgcgcagtac ggtgaccagg agtttaccgg
    26101 cgcagagcag gcggttgcgg atatcaacgc taaaggcggc attaaaggca acaaactgca
    26161 aatcgtaaaa tatgacgatg cctgtgaccc gaaacaggcg gttgcggtgg cgaacaaagt
    26221 cgttaacgac ggcattaaat atgtgattgg tcacctctgt tcttcatcaa cgcagcctgc
    26281 gtctgatatc tacgaagacg aaggcatttt aatgatcacc ccagcggcaa ccgcgccgga
    26341 gctgaccgcc cgtggctatc agctgatcct gcgcaccacc ggcctggact ccgaccaggg
    26401 gccgacggcg gcgaaatata ttcttgagaa agtgaaaccg cagcgtattg ctatcgttca
    26461 cgacaaacag caatacggcg aaggtctggc gcgagcggtg caggacggcc tgaagaaagg
    26521 caatgctaac gtggtgttct ttgatggtat caccgccggg gaaaaagatt tctcaacgct
    26581 ggtggcgcgt ctgaaaaaag agaatatcga cttcgtttac tacggcggtt atcacccgga
    26641 aatggggcaa atcctgcgtc aggcacgcgc ggcagggctg aaaactcagt ttatggggcc
    26701 ggaaggtgtg gctaacgttt cgctgtctaa cattgcgggc gaatcagcgg aagggctgct
    26761 ggtgaccaag ccgaagaact acgatcaggt tccggcgaac aaacccattg ttgacgccat
    26821 caaagcgaaa aaacaggacc caagcggcgc attcgtttgg accacctacg ccgcgctgca
    26881 atctttgcag gcgggcctga atcagtctga cgatccggct gagatcgcca aatacctgaa
    26941 agcgaactcc gtggataccg tgatgggtcc gctgacgtgg gatgagaaag gtgatctgaa
    27001 aggctttgag ttcggcgtat ttgactggca cgccaacggc acggcgaccg atgcgaagta
    27061 atcattaatc ggcaactttg ggttgccgcc aaattgctaa tatcgagtac gttgcttcat
    27121 gccggatgcg gcgtaaacgc cttatccggc ctacaagatc caaagaaatc agtaaattgc
    27181 aacacacatt gtaggcctga taagcgtagc gcatcaggca atttctcgtt ggtcatcact
    27241 tttgaaatca acttgacgat taacgcttct cccagccgcc ctgttgcgcc gtaaatccca
    27301 gtgcctgcat aaacgccgtc atcacaccgc gatcttccac gcctgcatcc gccatccacc
    27361 agcatgaaac gccaggattg ttacgcaaaa cctcttccag cagatattgc cccacaccgc
    27421 gacgtcgggt gacttcccgc acacgcaggg aatccagtgc tccctcggtg ccgcttaagg
    27481 ttacccgtac ggcagcgagc aggcgctcgt taaaacgcgc ggcgtagata cggtggttat
    27541 cgtcaacctg taaagaggaa ggggaatact ccggccagat cttttgcagg tcaatccggt
    27601 cctggtcgct aaagttttct aatcgaatga tggtcagctt catgggtaac ccgtgtaaat
    27661 cacaaaagtg gaaccagtgt agcgaaataa tttaatcgga ggctttctct tttttatttc
    27721 ttttggcagg tgattaattt tttaacagca ataattacaa agttaaaaca ttagagaatg
    27781 aaaaataccc agcataatcc cctgaatggt agtgaattat tccgcccttt gtgccgttat
    27841 tt
//
LOCUS       DAJVUJ010000038        27675 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.038, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000038 DAJVUJ010000000
VERSION     DAJVUJ010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27675
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.038"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(211..420)
                     /gene="fumD"
                     /locus_tag="OQQ48_001510"
     CDS             complement(211..420)
                     /gene="fumD"
                     /locus_tag="OQQ48_001510"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HCS1952329.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELDKQAMETVINALVK"
     gene            977..2389
                     /gene="pykF"
                     /locus_tag="OQQ48_001511"
     CDS             977..2389
                     /gene="pykF"
                     /locus_tag="OQQ48_001511"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HCS1952330.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            2700..2936
                     /gene="lpp"
                     /locus_tag="OQQ48_001512"
     CDS             2700..2936
                     /gene="lpp"
                     /locus_tag="OQQ48_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HCS1952331.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(3000..4004)
                     /gene="ldtE"
                     /locus_tag="OQQ48_001513"
     CDS             complement(3000..4004)
                     /gene="ldtE"
                     /locus_tag="OQQ48_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HCS1952332.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLLPVVPAGPNNPLGRYALRFAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRARQ
                     "
     gene            complement(4153..4569)
                     /gene="sufE"
                     /locus_tag="OQQ48_001514"
     CDS             complement(4153..4569)
                     /gene="sufE"
                     /locus_tag="OQQ48_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HCS1952333.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDRSP
                     QNSIQGCQNQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            complement(4582..5802)
                     /gene="sufS"
                     /locus_tag="OQQ48_001515"
     CDS             complement(4582..5802)
                     /gene="sufS"
                     /locus_tag="OQQ48_001515"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HCS1952334.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLHTL
                     FDEKTRLLAITHVSNVLGTENPLAGMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSVLGLNNIAEYELNLMHYALSQLESVPNLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTCLQRIHRLLG"
     gene            complement(5799..7070)
                     /gene="sufD"
                     /locus_tag="OQQ48_001516"
     CDS             complement(5799..7070)
                     /gene="sufD"
                     /locus_tag="OQQ48_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCS1952335.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTSLEGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMYITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLAEDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            complement(7045..7791)
                     /gene="sufC"
                     /locus_tag="OQQ48_001517"
     CDS             complement(7045..7791)
                     /gene="sufC"
                     /locus_tag="OQQ48_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCS1952336.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLAVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            complement(7801..9288)
                     /gene="sufB"
                     /locus_tag="OQQ48_001518"
     CDS             complement(7801..9288)
                     /gene="sufB"
                     /locus_tag="OQQ48_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCS1952337.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            complement(9297..9665)
                     /gene="sufA"
                     /locus_tag="OQQ48_001519"
     CDS             complement(9297..9665)
                     /gene="sufA"
                     /locus_tag="OQQ48_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HCS1952338.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            complement(10213..10401)
                     /locus_tag="OQQ48_001520"
     CDS             complement(10213..10401)
                     /locus_tag="OQQ48_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HCS1952339.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            complement(10501..10911)
                     /gene="menI"
                     /locus_tag="OQQ48_001521"
     CDS             complement(10501..10911)
                     /gene="menI"
                     /locus_tag="OQQ48_001521"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HCS1952340.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            complement(10908..13964)
                     /locus_tag="OQQ48_001522"
     CDS             complement(10908..13964)
                     /locus_tag="OQQ48_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCS1952341.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDVLDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDQEMLGLNIVEFAGDDEALIDERVNALCARLDELIASHQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPELDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTL
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLESLNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            14353..15465
                     /gene="ydiK"
                     /locus_tag="OQQ48_001523"
     CDS             14353..15465
                     /gene="ydiK"
                     /locus_tag="OQQ48_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HCS1952342.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            15651..15758
                     /gene="rprA"
                     /locus_tag="OQQ48_001524"
     ncRNA           15651..15758
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="OQQ48_001524"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            15894..16273
                     /locus_tag="OQQ48_001525"
                     /pseudo
     CDS             15894..16273
                     /locus_tag="OQQ48_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001014786.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
     gene            16218..16940
                     /locus_tag="OQQ48_001526"
                     /pseudo
     CDS             16218..16940
                     /locus_tag="OQQ48_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            16952..17818
                     /gene="ydiB"
                     /locus_tag="OQQ48_001527"
                     /pseudo
     CDS             16952..17818
                     /gene="ydiB"
                     /locus_tag="OQQ48_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
     gene            17850..18608
                     /gene="aroD"
                     /locus_tag="OQQ48_001528"
     CDS             17850..18608
                     /gene="aroD"
                     /locus_tag="OQQ48_001528"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCS1952343.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVACAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            18754..20349
                     /locus_tag="OQQ48_001529"
     CDS             18754..20349
                     /locus_tag="OQQ48_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HCS1952344.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SELAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQSQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFNFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIIFCGTLTAGSLKTEIADGKLNIVQEGRVKKFIQELPEI
                     TFSGKIALERELDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
     gene            20363..21514
                     /locus_tag="OQQ48_001530"
     CDS             20363..21514
                     /locus_tag="OQQ48_001530"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HCS1952345.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFAEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(21557..22468)
                     /locus_tag="OQQ48_001531"
     CDS             complement(21557..22468)
                     /locus_tag="OQQ48_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCS1952346.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKNAYRSEQGYIKKDVLIKDILFYLSNNYREKITLEQLSKKFRASVSYICHEFT
                     KEYRISPINYVIQRRMTEAKWSLTNTELSQAEISWRVGYENVDHFAKLFLRHVGCSPS
                     DYRRQFKNCFAEQEILSEFPQPVSLVG"
     gene            22782..23553
                     /locus_tag="OQQ48_001532"
                     /pseudo
     CDS             22782..23553
                     /locus_tag="OQQ48_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
     gene            23573..24511
                     /locus_tag="OQQ48_001533"
     CDS             23573..24511
                     /locus_tag="OQQ48_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HCS1952347.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAAPLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            24567..25856
                     /locus_tag="OQQ48_001534"
     CDS             24567..25856
                     /locus_tag="OQQ48_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCS1952348.1"
                     /translation="MSDDKFDAIVVGADVASSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILQANVVVLAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQMVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KEREDFSASSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYSRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            25853..26146
                     /locus_tag="OQQ48_001535"
     CDS             25853..26146
                     /locus_tag="OQQ48_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCS1952349.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            26203..>27675
                     /gene="fadK"
                     /locus_tag="OQQ48_001536"
     CDS             26203..>27675
                     /gene="fadK"
                     /locus_tag="OQQ48_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HCS1952350.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGATYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVSVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSPIIADNTSLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNVLEKQPADLSALRFFLCGGTTIPKKVARECQQRGIKLLSVY
                     GSTESSPHAVVNLDDPLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYCSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSL"
BASE COUNT         6906 a         7001 c         6802 g         6966 t
ORIGIN      
        1 aactgatatt tattatcatt tgaaataaat ttaacttaag cgacaattat ttgtgacgtt
       61 cattgcagaa aataaaaata atattatcgt tataaattaa aatacatacc gcaattcgta
      121 taatttcagc agttgttatt tagaatgatt aactggaccg caactgaagc aagaatatca
      181 ggatgaagct taccgcctca tcctgaatgg tcattttacc agtgcgttaa tgacggtttc
      241 catcgcctgc ttatccagct cactgcgctg aatgcgttta ttggctaatg ctgtacgtaa
      301 tacgcctgcg ataacaatat gctttggttc ctgccccaga tcgcgcattt ccagcacaac
      361 tttaccgaca actctgcaca tttctcggta cagttcatcc tcttttgtcc gatttcccat
      421 tctttatacc tatttatcat gccaactatc agcatatatc aatctaacca attaaacaaa
      481 aatcaaacaa aatcagacaa ataacgcgat aaattatttt aactgtagca attgagcgat
      541 gatatattta tacaccggat gaactttcac ttatcctcac actgacaact ttggcaccag
      601 acgttgcgca aacagcgaag tttttgcgta accttttccc tggaacgtta aatctttgat
      661 aacaatttat tgtctaacaa gttgtatatt ttttgaaacg ctgtttttgt tttccttttg
      721 gattaatttc agcgtataat gcgcgccaat tgactcttga atggtttcag cactttggac
      781 tgtagaactc aacgactcaa aaacaggcac tcacgttggg ctgagacaca agcacacatt
      841 cctctgcacg ctttttcgat gtcacttatc cttagagcga ggcaccacca ctttcgtaat
      901 accggattcg ctttccggca gtgcgcccag aaagcaagtt tctcccatcc ttctcaactt
      961 aaagactaag actgtcatga aaaagaccaa aattgtttgc accatcggac cgaaaaccga
     1021 atctgaagag atgttagcta aaatgctgga cgctggcatg aacgttatgc gtctgaactt
     1081 ctctcatggt gactatgcag aacacggtca gcgcattcag aatctgcgca acgtgatgag
     1141 caaaactggt aaaaccgccg ctatcctgct tgataccaaa ggtccggaaa tccgcaccat
     1201 gaaactggaa ggcggtaacg acgtttctct gaaagctggt cagaccttta ctttcaccac
     1261 tgataaatct gttatcggca acagcgaaat ggttgcggta acgtatgaag gtttcactac
     1321 tgacctgtca gttggcaaca ccgtactggt tgacgatggt ctgatcggta tggaagttac
     1381 cgccattgaa ggtaacaaag ttatctgtaa agtgctgaac aacggtgacc tgggcgaaaa
     1441 caaaggtgtg aacctgcctg gcgtttccat tgctctgcca gcactggctg aaaaagacaa
     1501 acaggacctg atctttggtt gcgaacaagg cgtagacttt gttgctgctt cctttattcg
     1561 taagcgttct gacgttatcg aaatccgtga gcacctgaaa gcgcacggcg gcgaaaacat
     1621 ccacatcatc tccaaaatcg aaaaccagga aggcctcaac aacttcgacg aaatcctcga
     1681 agcctctgac ggcatcatgg ttgcgcgtgg cgacctgggt gtagaaatcc cggtagaaga
     1741 agttatcttc gcccagaaga tgatgatcga aaaatgtatc cgtgcacgta aagtcgttat
     1801 cactgcgacc cagatgctgg attccatgat caaaaaccca cgcccgactc gcgcagaagc
     1861 cggtgacgtt gcaaacgcca tcctcgacgg tactgatgca gtgatgctgt ctggtgaatc
     1921 cgcaaaaggt aaatacccgc tggaagcggt ttctatcatg gcgaccatct gcgaacgtac
     1981 cgaccgcgtg atgaacagcc gtctcgagtt caacaatgac aaccgtaaac tgcgcattac
     2041 cgaagcggta tgccgtggtg ccgttgaaac tgctgaaaaa ctggatgctc cgctgatcgt
     2101 ggttgctact cagggcggta aatctgctcg cgcagtacgt aaatacttcc cggatgccac
     2161 catcctggca ctgaccacca acgaaaaaac ggctcatcag ttggtactga gcaaaggcgt
     2221 tgtgccgcag ctggttaaag agatcacttc tactgatgat ttctaccgtc tgggtaaaga
     2281 actggctctg cagagcggtc tggcacacaa aggtgacgtt gtagttatgg tttctggtgc
     2341 actggtaccg agcggcacta ctaacaccgc atctgttcac gtcctgtaat attgcttttg
     2401 tgaattaatt tgtatatcga agcgccctga tgggcgcttt ttttatttaa tcgataacca
     2461 gaagcaataa aaaatcaaat cggatttcac tatataatct cactttatct aagatgaatc
     2521 cgatggaagc atcctgtttt ctctcaattt ttttatctaa aacccagcgt tcgatgcttc
     2581 tttgagcgaa cgatcaaaaa taagtgcctt cccatcaaaa aaatattctc aacataaaaa
     2641 actttgtgta atacttgtaa cgctacatgg agattaactc aatctagagg gtattaataa
     2701 tgaaagctac taaactggta ctgggcgcgg taatcctggg ttctactctg ctggcaggtt
     2761 gctccagcaa cgctaaaatc gatcagctgt cttctgacgt tcagactctg aacgctaaag
     2821 ttgaccagct gagcaacgac gtgaacgcaa tgcgttccga cgttcaggct gctaaagatg
     2881 acgcagctcg tgctaaccag cgtctggaca acatggctac taaataccgc aagtaatagt
     2941 acctgtgaag tgaaaaatgg cgcacattgt gcgccatttt ttttgtctgc tatttaccac
     3001 tactgcctcg cgcgtaacat attcccttgc tctggttcac cattctgcgc tgactctact
     3061 gaaggcgcat tgctggctgc gggagttgct ccactgctca ccgaaaccgg ataccctgcc
     3121 cgacgataca acgctttatc gactaacttc tgatctacag ccttattgtc tttaaattgc
     3181 gtaaagcctg ctggcagtgt gtatggcatt gtctgaacgt tctgctgttc ttctgccgat
     3241 agtggtcgat gtacttcaac ataacgcatc ccgttaggct ccacggaata tttcaccggt
     3301 tcgttgatca ctttcaccgg cgttcccgtc cgcacgctgg agaataaggc tttaatatcc
     3361 ggtgcattca tgcgaataca gcccgaactg acgcgtaaac cgacgctgtc cggcgcactg
     3421 gtaccatgaa tgaggtattc gccattacca tgcgcgaagc gcagtgcgta acgtcctagc
     3481 gggttatttg gtccggcagg aacgactggc agtaatttaa tgccacgctc cagcgaacgt
     3541 tgacgaatgc ctgccgtagg cgtccaggtt ggattaggga ttttctgccc cacacgcgtt
     3601 tccatcaccg gcgtttccag cccctgcaag ccaataccta tcggataaac ctgcacgata
     3661 ttttctcccg gcggataata ataaaggcgc agctctgcaa ggttaacgat aatcccctga
     3721 cgcggtgcat caggtaataa cagttgtgaa ggaatagtta tcgtcgtacc aggttttggc
     3781 accggggcga tagtgttatt ggcttcaagg atcaacattg ccgcagtatc aaaacgtcgg
     3841 gcaatagcct gaaggttttt atccccttct tgcaccgtat acgtttgatt ttgcccaacc
     3901 agtcggcttc cggttggtgg tagcggataa tcaaccgccc aggcagcctg gatggcgcta
     3961 aaagcgccga taagcgtgag tgtaagcaaa gacgcgcgtt tcattgtaaa cctcctgtat
     4021 ttgccggaga ctcacgctga aacgtcggat ggcgcttatg ttcacctgaa accaaaacac
     4081 tcctgtgcag gtcagtgtaa acattgacca tccggcaatg tgagccaacc ggatgaaagc
     4141 tgtcccttta gtttagctaa gtgcagcggc tttggcgcga attgcgcgaa tcatcgcttc
     4201 cagaccttgt gaacgagatg gggtgagatg ttgggtgagc gccatttttt caaaccacgg
     4261 acgcacatcg aaattgacaa tatcctgcgg cgtcatctga tcgtagagaa taaatacgac
     4321 cgcaataagc cctttcacaa tcgccgcatc gctgtcgccc tgcaattcaa taattccctg
     4381 cgcattctgg cgcatgacaa tccacacctg attctggcag ccctgaatgc tattttgtgg
     4441 gcttcggtct tcgtcgcgta attctggcag acgctggccc agctcaataa tgtagagata
     4501 tttctcttcc cagttggcgc agcgtaaaaa attacgcagc aatttttctt tatccggcaa
     4561 taaagccata gtgcctccct gttatcccag cagacggtga atacgttgca ggcaggtcac
     4621 cagacgatcc acttcttcat gagtgttata catggccagc gacgcccgac acatcgcagg
     4681 gacgttgtaa taggccatta atggcatagc gcagtgatgt ccggtacgca cagcaatgcc
     4741 gtaattatcg agaaaactgc caacatcata ggcgtggtgt ttaccgagat taaaagcaat
     4801 aacgccaagc ctgttttgtg ggccatagag agtgagattc ggtacagatt ccagctgtga
     4861 tagcgcgtaa tgcatcagat tcagttcata ctcggctatg ttattaagcc ccagcaccga
     4921 aacatattcc agcgccgcgc caagaccaat gatgcccccg gtattgggtg taccggcttc
     4981 aaaccgccat ggtgctttgg tccaggtagt gccttcactc aggctgacgg tggcgatcat
     5041 agaaccgccc ccttcccacg gcggcatctc ctgcaacaag gcttctttga cataaagaat
     5101 gccaatcccg gtggggccat acagtttatg cccggaaaac acgtaaaaat cgcaatccag
     5161 cgcctgaaca tccaccggat gatgcatcac cgcctgagcg ccatccacca gcacttttgc
     5221 gccatgctgg tgcgcaagcg tgatcattcc cgccagtgga ttttctgtgc caagcacgtt
     5281 ggagacatga gtaattgcca gcaggcgagt tttctcatca aacagcgtat gcagcgtctc
     5341 cagttgcagc gtaccgtcgg ggttgagagg gatcacacgc agctctgcgc caacgcgtgc
     5401 gcaaagcatc tgccagggga caatgttagc gtggtgctcc atctgactga tgatgatgtt
     5461 atcgcccgcc cgcacattgc tgttgcccca gctattggcg accagattga tcccttccgt
     5521 cgtgccgcgg acgaacacca gctcttccgc cgaacgggca ttaataaaca gcgatgcccg
     5581 cttgcgcacg ttctccattt tctcggtcgc ctgggcgctt aaggtatgaa taccgcgatg
     5641 caccgccgcg tagccatgac gataaaactc ggcctcggcg tcaatcacct ggctcggttt
     5701 ctgtgcactg gcggcgctgt cgagataagc cagcggcaaa ccgtttacct cacgcgaaag
     5761 caccggaaag tcggcccgca ctttgtcgac ggaaaaagtc atcttgcacc tcctggcagc
     5821 cgttgaccga ttcgggccag cacctgctgt ttaagcccct catcacgcag tgcttccgtc
     5881 agttcggcag caaaggcgta aatgatcatc tgctgggcat cctgctgatt gatcccgcgc
     5941 gagcgcagat agaacatctg ttcatcatca atacgcccca ccgtcgcgcc gtggctgcat
     6001 ttcacatcat ctgcatagat ttccagctgc ggtttcgtat ccacttccgc cagtttgccc
     6061 atcagcagat tattgttggt catctgacca tccgttttga tggcgtgctg cgcgacgttg
     6121 atcaaaccgt taaataccgc gcggcctttg tcgctgacga tagttttgtg caactgtcgg
     6181 ctgttacaaa aacctttatt gtgttccagc caggtacggg tatcacacac ctcgtttttc
     6241 accggcatcg ccaggctatt gatccgcagc gtgctgtttt cgccattgag ttgcgtactg
     6301 gtgttgtgtc gtaacactgc gccacccagc aggaaactat ggctaaatgc ggtggcatcc
     6361 tcagccagca acaaatcgtt atgagcaaag tggtgactga gcgggttttc aaacgccagc
     6421 ttgatatgct gcaagtgggc gttcgctgcg acattgatag tgaaccgtgc cccggtaaaa
     6481 tgacgagcat cattaaggct gacaaaatgt tcgatcaccg ttgcttcggc accttccgcc
     6541 agatccagat gatgccggta atgggcagtg ttcacctctt cacctgccac gccctgggtg
     6601 atatacatta acagcaatgg ctttgccggc cgttgaccgc gcttcacggc gatatgcgtc
     6661 acgctttgtg ccaggctttc cgtcaaatgc agaaacactt ccgcctgaat agcgtcgggt
     6721 aacccctgac ggtcgtcgtt aatgctcact tcatatccgc tgccttcaat tgcatcgctc
     6781 agcgcgggca cgtaacgtcc atcgacaaac accagccgca cggcgtctaa cgttaacgct
     6841 aaggcatcac gctgctgtgg ggatatctcc cccgcaatgc tgacaaactg gctattggtc
     6901 agcccttcca gcgacgtata tttccagttt tcatgtttac gtgtcggcag tccggtacgc
     6961 agcaattgtt gtaaatgctg ctgtgcttgc ggggagcgtt ttgtcccttc agcttcaaac
     7021 aagtgatgcc actgttgcag cgcgttactg ctgttcggta agccagccat aaccctgctc
     7081 ctccagttgt ttgaccaacg tgaaatcgcc ggatttcaca attcgtccct gatatagcac
     7141 atggacgtaa tcaggcttga tgtagtcgag aatgcgttgg tagtgcgtaa caatgatgaa
     7201 tgagcgcttg ccatcacgca gggagttcac gccatcggcg accactttta atgcgtcaat
     7261 atccagcccg gagtccgact catcaagaat gcataactcc ggttccagca ccgccatttg
     7321 caaaatatcg ttgcgctttt tctcgccgcc ggaaaaacca acgtttaccg aacgggttaa
     7381 taaatcttcc ggcatcttca ggagagcgat tttctcttcc atcaaatcct gaaaatcaaa
     7441 gcggtcgagc gtttcctggc cgcgatagct gcgcactgca ttaagtgccg tttgcaggaa
     7501 aaactggtta ctgacgcctg gaatctccac cggatactgg aaggccataa agatgccttc
     7561 gcctgcacga tcttccggcg acagtgcaag caaatctttg cctttgaact caaccgtgcc
     7621 gcccgtcact tcataatctt cgcgcccggc aagcgttgcc gataaggtac ttttgcccga
     7681 accgtttggc cccataatgg cgtgaacttc gccgggacga acggcgaggc ttaatccacg
     7741 caggatagct ttatcttcca cgctgacgtg taaatcttta atacttaaca tgtttattcc
     7801 ttatccgacg ctgtgttcaa gactgatcgc gaggagtttt tgtgcttcaa cggcaaattc
     7861 cagcggcagt tctgagaaca cgtctttgca gaaaccgtta acaatcatcg agatggcgtc
     7921 ttcttcgctg atcccgcgtt gcaggcagta aaacagttga tcttcaccaa tacgtgatgt
     7981 cgttgcctcg tgctccagtt gtgcgctgtt attgcgacat tcgacatacg ggaaggtatg
     8041 cgccccacaa ttagcgccaa tcagcattga gtcgcactga gtgaaattgc gcgcattggt
     8101 tgccgtcggc atgattttca ctaagccgcg ataactgttc tgactatgtc cggcagagat
     8161 ccctttcgag ataatggtcg atttggtgtt tttaccgatg tggatcatct tggtgccggt
     8221 atccgcttgc tgatgaccgc tggtcagcgc cactgagtaa aactcaccaa tggagttatc
     8281 gccgcgcaaa atgcagctgg gatatttcca cgtaatcgcg gacccggttt ctgattgcgt
     8341 ccatgacatt ttgctgtttt cgccttcgca caaagcacgc ttggtgacga agttgagaat
     8401 accgccggtg ttgttatcgc cagggaacca gttttgtacc gtggaatatt tcacctcggc
     8461 gtttttatgg atgatgactt ccaccactgc cgcgtgtaac tgatagctgt cacgcaccgg
     8521 agcggaacag ccttcaatgt agctgacgta gctgtcttcg tcggccacca gaatggtgcg
     8581 ctcaaactgc ccggtttttt ccgcgttaat gcgaaaatag gtggaaagtt ccatcgggca
     8641 gcgcacgcct ttaggcacat aaataaacgt accgtcagag gctaccgccg cattaagcgc
     8701 ggcaaagaag ttgtcattcc ccggcaccac ggtgccgaga tatttacgca ccagttccgg
     8761 gtgatcgtgg atcgcctcac caaaggaaca gaaaataatt ccctgctccg ccagtttttc
     8821 gcgataagta gtggcaaccg aaacagagtc gaaaatggca tccaccgcca cctctttgcc
     8881 ttcccgcacg ggaacgccca actgctcaaa cgccgcctcc acctctttac ttaaaaaggc
     8941 gttcgcgcca gtttgctgca ccgcgccagg ttcagacgcg caagtgtcgt cacaattacc
     9001 gcacgatggt gctgagtagt agctgtaatc ctgataattc agcttgtcgt agtgcgcttt
     9061 caaccagtgc ggttcttcca tctccagcca tgcgcgatag gcgtttagac gaaactccag
     9121 catccactcc ggctcattac gcttcgccga aattgcgcgc accacctctt cgtttatccc
     9181 ctttgccagc tcatcggtgg ctaactgggt gaagaatcct tctttataat tcagcgggcc
     9241 gccggtccag gttttgacat cgtcagttgc ttcagtatta cgagacatag taccgcctat
     9301 accccaaagc tttcgccaca gccacattca ttctgggctt tagggttgtg aaatttgaat
     9361 atctgattaa gtccttcacg aacgaaatcg acttccgtgc catcaataaa cggcatcgct
     9421 tgcagcggga caaacagctt cgcgccgtcg tgttcaaaca gcagatcgtc tttgtccggc
     9481 tcgctaacac tgtcgagcac atagccaaag cccgcgcagc ccgtttgctt cacgcctaaa
     9541 cgcacgccga ccatacccgg ctgctttgcc accagctcac ggatgtgtat cgccgctgcg
     9601 ggtgtcagcg ttaagccttg ccaggcgaaa tcttgtgggt taaaggttcc tgaatgcatg
     9661 tccatcgatt tacctcactt catcgctttc agcgtataac agcatgttgg tgataatgat
     9721 tatcagttca acccagcaaa cgcaggggct ttaccgtaaa acatgctttt tgcctgcttt
     9781 taataagcat agaccctgat gtgtgggtta acaggcacgc taagaattag cgtatctcat
     9841 tgttagataa tggttattat ctaaggagca ttaaggctgt gatgaaaaag aaaaagttgt
     9901 attaaaatga ctatttaaga gataggtaaa aaagtgcagc gttcagaaat aagaaaaccc
     9961 ttaagtctgt gcgacacagg cttaagggtt tctaccccat ccggcgctta tctccggcac
    10021 tctcagtggc ttagctcttg aaggggcgat aagaataatc tcataaagct aacccgccgt
    10081 tttaacacaa actgcgatta gtattatttt tgaacaatat caggcggtag ataagcagta
    10141 ttaagaaggt catcgaacct ggacggaggt taatccaggt cgatttggcg aacttgcggc
    10201 attaagtcag gatcaatgca cgcccagacg ccaggcaaag tagatttctt cttttaattc
    10261 agcagaagag agagtaagca ggtcagcaaa ttcaagttct agttgtttca gacgtttgag
    10321 atattgggca ggtgaaagat tgctctggtc acggcgtaaa aattcaatgg ccagctgggt
    10381 gggatcaagt tgagtagaca tagcatcctc gcttttagac aagacgtgta cagtatacca
    10441 ccgtttactg tgcagataat gaccaaaagc aatatgcgtc acacttttct ggtgacaacg
    10501 tcacaaaatg gcggtcgtca atcgtgacga acagcacaaa cgccctttct catcgaagat
    10561 ttcaatctgc cagacctggt gacgcgaacc gagatgcaac ggtttacata tgccgcgcac
    10621 ccgcccttct cgtgccgagc ggacgtgatt agcattgatt tccagaccaa ccactttttg
    10681 ctcaccttcg gtacataaat aaccggcaac ggaaccgata ctttcggcca gtaccacgga
    10741 tgctcctcca tgcagcaacc cgaaaggctg ctttgtccgc gagtctactg gcattgtcgc
    10801 ttcaagggtg tcatcaccaa tatgttcaaa gcgaatatcc aggaacccca ccatgtttcc
    10861 ttcacccata gcattcagtg cttccagggt gattttccgt ttccatatca tttaataatc
    10921 tccagtaaag cctgcacagg atggcgtacc cccgtgcctt caacccgttt tacctggcta
    10981 cggcaggaat atccggtcgc cagacagcgg ttacgcggca gtcgctgcat agcctgatgc
    11041 caggataact catagatccc gagcgaattt tcatggtttt tcgcttcatg tccgtaagtc
    11101 cctgccatgc cgcagcaacc cacgctgaca ttttccagtt tcgcgccaaa acgggcaaat
    11161 atcgcggccc attgtgctgg cgcacccggc aaggcggtaa cttcggtaca gtgaccaaag
    11221 aaataccatg attcaccgct gactgtagcc acaggctgtg actcaagtgc gcttgccagc
    11281 cattcattcg ccagtaagac gttaaactcg ccacgctcct cgcccagcgc cagtttatat
    11341 tcatcgcgat aacaaagtac cagcgccgga tcgacgccca ccattggcat acccagcttc
    11401 gccatacggt tgaggaaatc cgccgtcttt ttcgccgtct tcgcaaaacg attaagaaaa
    11461 cctttaatat gctgggcttt gccatttggc gaaaatggca gtaacacagg ctggaaacct
    11521 aatttttcga ccagacggac aaaatccgcc accacttgcg catcgtaata gctggtaaag
    11581 gggtcctgca ccaccaacac tgtgcgcgct ttctgctctg cattgagaga ttcaagctgt
    11641 tccagcgtca tgtttgccga gcgatgcccc accatttgtt gttgtagcga ggggaccgac
    11701 agcagcggca aatcaaccat gccgatatgt ttttccgaga gtttgcgcac cagcggctgg
    11761 ttaatgaaga agttaaaggt tttcggcgcg cgtgccatca gcggcgcgta gctctcgacc
    11821 gtagcgacga ggtggtcgcg cagcgggcgt aaataacggg tgtgatagag ctgcagaaaa
    11881 cgagagcgaa actccggcac atcaattttg atggggcaca gggtcgaaca cgctttacag
    11941 gccagacagc ccgacatcgc ctctttgact tcgtgtgaga agtcatattc gcctttattc
    12001 gcatgccagc tattgcgcgt gcgggcaatt aacgtccgca aactgacgcc ggattcaggc
    12061 agttcttgtt ccagtttgag tggatcaacg ccgcgatccg ccaacaaacg cagccattca
    12121 cgcaccagcg ttgcgcgccc tttcggtgaa tgaatccggt tctgggtgat cttcatcgac
    12181 ggacacatcg gactacgggc atcaaagttg aagcataaac cgttgccgtt acactccatc
    12241 gcaccgcgcc actgctggcg taccgcaatg gggatctgcc gatcgaatgt accgcgcttc
    12301 accgcgtcca ctttcatcat cggcgcatcg agaccttctg gcgggcaaat cttccctggg
    12361 ttgagtcggt tatgcgggtc aaatgccgct ttcactttgc gcagttctgc aaaaagttcc
    12421 tcaccgaaaa acgccgggct gtattcagcg cgaaaacctt tgccgtgctc gccccacaac
    12481 aaaccaccgt atttcgcagt cagcgccacc acgtcatcag agatttgctt catcaaaatc
    12541 tcttgttgag gatcgcacat atccagctct ggacggacgt gcaagacacc tgcgtcgacg
    12601 tgaccgaaca taccgtagct taagccgtgg ctgtcgagca gcgcgcgaaa ttcagcaata
    12661 taatccgcca ggtgttccgg cggtacgcag gtatcctcag caaacggaat tggcttagcg
    12721 gcacctttgg cattgccaag cagaccaacg gcttttttgc gcattgcata gatacgttca
    12781 acccccgcca gctcacggca cacctgccag ccgatgacac ctgcttgatg actggcgatc
    12841 agctcatcca accgcgcaca gagtgcattt acccgctcat caatcagcgc ctcatcatca
    12901 ccagcaaatt ccacaatgtt cagcccgagc atctcttggt caggcacatc ggtaatcaac
    12961 tcgctgacgg aatgccagac aatatcttcc cgcgccagat tcagcacttt tgagtccacc
    13021 gtctctaccg aaagcgcccg cgcctcaacc ataaacggcg cgttacgcag cgcagagtca
    13081 aaagagtcat atttgacgtt caccagacgg cgcactttag gcaagcgcgt aatatccagc
    13141 cgcgcttcgg taataaaggc cagcgtccct tctgaacccg tcagaatgcg cgtcaggtcg
    13201 aactcggtca tctcatcgtt aaagacatga cgcagatcgt aaccggtaag aaagcggtta
    13261 agtttgggga agttgtcgat aattaactgg cgttgctgac ggcaacgttg ataaaccgtg
    13321 ttataaattc gcccgattgt ggtattggat ttacccagcg tttccgccaa ttcgacgggt
    13381 aaaggttgcg tatcgagaac atcgcccccc aacaacaccg cgcgtacgcc aagtacgtga
    13441 tctgacgttt tgccatagac cagcgatccc tgaccggatg catcggtatt gatcatccca
    13501 ccgagcgttg cccggttgct ggtcgaaagt tccggcgcaa aaaagtagcc gaacggtttc
    13561 aggtactgat tgagttgatc ttttatcacc ccggcctcaa cgcgcaccca gccctcttca
    13621 gggttaattt cgatgatgcg gttcatatgg cgggacatat caacaataat cccctggttg
    13681 agcgcctgac cgttagtgcc ggtgccgccg ccgcgggggg taaagatcag cgatgaatag
    13741 cgttcctgcg cggcaagacg ggcgatcagc gccacatctg cggttgaacg cggaaatacc
    13801 accgcatcgg ggagaagttg gtaaatactg ttgtcggtcg acattgtcag acgatcggca
    13861 taacttgtcg ccgtatcgcc ggtaaaacct tgttgctcca gctcttgcaa aaaattaagc
    13921 accagttgaa cgacgccggg tgcctgagaa atctgtggaa tcattatatt gcccctttcc
    13981 tgcggtctgt gatgtaggtc gatacactat tctttcaggc ggctgcaata gcgcactgaa
    14041 aggtgatgtt tatttactct atggatttcg agttgcagga aggcggcaag cgagtgaact
    14101 ccaggagctt acaatagtaa gtgactgggg tgaacgaacg tagccgcagc acatgcaact
    14161 tgaaatacga cgagtaaatc gtttgcgtgt tgcctgagtt gttgtaccac atttttttct
    14221 aacacgccca tcagaattaa gggcagaatc ggcctgttaa aaaccgctga aattgctcat
    14281 cattatgcag gtgagtttcg cgtgttcacg tcgcgtcgac gatttgacgc acaaaaaagg
    14341 tgaaaagtag ttatggtaaa tgttcgtcag cccagggatg tcgcacaaat tctgctttcg
    14401 gtgctgtttt tagccatcat gattgtggca tgtctgtgga ttgttcaacc ctttattctc
    14461 ggctttgcat gggccggtac ggtggttatc gccacctggc cggtattgtt acgtttgcaa
    14521 aagatcatgt ttggccgccg ctctctcgcc gttctggtga tgacgctgtt attagtgatg
    14581 gtgtttatca tccctattgc tttgctggtt aacagtatcg tcgacggcag cggcccgcta
    14641 attaaagcca tttccagcgg cgacatgacg ttacccgatc tggcgtggct taataccatt
    14701 ccggtgattg gcgcgaagct gtatgcaggc tggcacaact tgctggatat gggggggacg
    14761 gcgatcatgg cgaaagtccg cccttatatt ggcaccacca ccacctggtt cgttgggcag
    14821 gcggcgcata tcgggcgctt tatggtgcat tgtgcgctga tgcttctctt cagtgccctg
    14881 ctgtactggc gcggtgaaca ggtggcacaa ggcattcgcc attttgcaac ccgtctggca
    14941 ggcgttcgcg gagatgccgc cgtactgctg gcggcacagg ctatccgcgc ggtggcgctg
    15001 ggtgtggtgg tgacggcgtt agtacaggca gtgcttggcg gtatcggcct cgccgtatcc
    15061 ggcgtacctt atgcaacgtt gctaacggtg ttaatgatcc tctcctgcct tgtccagctt
    15121 ggcccgttgc cggtactgat tccggcgatt atctggctct actggactgg cgataccacc
    15181 tggggaacgg tattgttagt gtggagcggt gtggttggca cgctggataa cgtcatccgc
    15241 ccaatgttaa ttcgcatggg tgccgattta ccgctgatcc tgatcctctc tggtgttatt
    15301 ggtggtttga ttgctttcgg gatgatcggt ctgtttattg gtccggttct gttagccgtt
    15361 tcctggcgtc tgtttgccgc gtgggtggaa gaagtcccgc cgccgacgga ccaaccggaa
    15421 gaaattctcg aagaacttgg cgaaatcgag aaaccgaata agtaatttct catcaggcgg
    15481 ctctgccgcc tgattgttaa cccccgctaa ttgatatttg cttgctcttc cccatatttt
    15541 cctgcttacc gccattcagc tggtagtacc tgtcgcaaat tctttacagt ttttaaacta
    15601 atgagacgaa tctgatcgac gcaaaaagtc cgtatgccta ctattagctc acggttataa
    15661 atcaacatat tgatttataa gcatggaaat cccctgagtg aaacaacgaa ttgctgtgtg
    15721 tagtctttgc ccatctccca cgatgggctt ttttttaaca tttttccact tcgctacctc
    15781 gcccctcact ctttccaata atgttgcaca agatactgtg actctcttca agccgtcagc
    15841 aggctgataa tgttatttat attttattgt ttatttcatg cagccattac acgatgaacg
    15901 cttatgaact ccaggcactc cgacatattt ttgccatgac tattgatgaa tgcgcacctg
    15961 gattgcccag accggtgaca gtgaaagctg gcgtcagtgg gaaaatggca agtgtgccat
    16021 tcctgatcgt gtagtcgagc aactgttggc tatgcgtcag caaagaaaaa aacatcttca
    16081 tgccatcata gagaaaataa ataaccgcat cggcaacaac accatgcgtt ttttccccga
    16141 cttaaccgca tttcaacgag tctaccctga tggcaatttt attgactgga agatctatca
    16201 atcggtagcc gctgaaggta atgctgttga aaagcaaatt ccccagccag ttggtggacg
    16261 ccagcgtagc taatgaatta ccgcaaatga acagcaaacc gttagtctgg ctggaaggtg
    16321 tttcatcggt actgttcggt gtagccgcat tctcgacctt ttatgtgatt gtggtgtgga
    16381 tgcccaaata tgcgatggct tttgctggta tgtcagaagc tgaggcatta aaaaccatct
    16441 cttattacag tatgggctcg ttggtctgtg tctttatttt tgccgcacta ctgaaaaaaa
    16501 tggtccggcc catctgggct aatgtattta actctgcact ggcaacaata acagcagcca
    16561 ttatctatct gtacccttct ccactggtgt gtaatgccgg cgcctttgtt atcggtttct
    16621 cagcagctgg cggcatttta cagctcggtg tttcggtcat gtcagagttt ttcccaaaaa
    16681 gcaaagccaa agtcaccagt atttatatga tgatgggtgg actggctaac tttgttattc
    16741 cgctgattac cggttatctg tcgaacatcg gccttcaata tatcattgtt ctcgatttta
    16801 ctttcgcgct gctggccctg attaccgcaa ttattgtttt tatccgctat taccgcgttt
    16861 ttattattcc tgagaatgat gtgcggtttg gcgagcgtaa atttagcacc cggttaaaca
    16921 caattaagca tagaggttaa aggagttaat tatggatgtt accgcaaaat acgaattgat
    16981 tgggctgatg gcctatccta tccgccacag tttatcgcct gaaatgcaga ataaagcctt
    17041 agaaaaagcg ggattgccat ttacctatat ggcctttgaa gtggataacg atagttttcc
    17101 cggagcaatt gaaggattaa aagctctaaa aatgcgcgga actggtgtat caatgccgaa
    17161 caaacaactg gcttgttaat atgttgatga attaacgcca gcagccaaac tggtgggtgc
    17221 catcaacacc atcgttaatg atgatggcta cctgcgcggc tataacaccg acggcacggg
    17281 ccatattcgc gccattaaag agagcggttt tgatatcaaa ggcaaaacaa tggtgctgtt
    17341 aggggccgga ggtgcctcaa cggctattgg cgcgcagggg gcaattgaag gtttaaaaga
    17401 aattaaactc tttaaccgtc gggatgagtt cttcgataaa gccctcgcct tcgcgcagcg
    17461 ggttaacgaa aacaccgatt gtgtcgtcac ggttaccgat ctcgccgatc agcaagcctt
    17521 tgctgaagcc ctggcttccg ccgacatttt aaccaatggc acaaaagtgg gtatgaaacc
    17581 ccttgagaat gaatcattag ttaatgatat cagtctgtta catccgggac ttctggtcac
    17641 tgaatgcgtg tataacccgc atatgacgaa gttattgcag caggcgcaac aagctggttg
    17701 caaaacgatt gatggatacg gcatgttgtt gtggcaaggg gctgaacagt tcacgttatg
    17761 gactggcaaa gatttccctc tggaatatgt taaacaggtc atggggttcg gtgcctgaca
    17821 ggctgaccgc gtgcagaaag ggtaaaaaaa tgaaaaccgt aactgtaaaa gatctcgtca
    17881 ttggtacggg cgcacctaaa atcatcgtct cgctgatggc gaaagatatc gcccgcgtga
    17941 aatccgaagc tctcgcctat cgtgaagcgg actttgatat tctggaatgg cgtgtggacc
    18001 actttgccga cctctccaat gtggagtctg tcatggcggc ggcaaaaatt ctccgcgaaa
    18061 ccatgccaga aaaaccgctg ctgtttacct tccgcagtgc caaagaaggc ggcgagcagg
    18121 cgatttccac cgaggcttat attgctctca atcgtgcagc catcgacagc ggcctggttg
    18181 atatgataga tctggagtta tttaccggcg atgatcaggt taaagaaacc gtcgcctgcg
    18241 cccacgcgca tgatgtgaaa gttgtcatgt ccaaccatga cttccataaa acgccggaag
    18301 ccgaagaaat cattgcccgt ctgcgcaaaa tgcagtcctt cgacgccgat attcctaaga
    18361 ttgcgctgat gccgcaaagt accagcgatg tgctgacgtt gcttgccgcg accctggaga
    18421 tgcaggagca gtatgccgat cgtccaatca tcacaatgtc aatggcaaaa actggcgtaa
    18481 tttctcgtct ggctggtgaa gtatttggct cggcggcaac ttttggtgcg gtaaaaaaag
    18541 cctctgcgcc agggcaaatc tcggtaaatg atttacgcac agtattaact attttacatc
    18601 aggcataagc aataatattt cggcgggaat atcctccccc gccgaactaa aaaaaatata
    18661 ttcaaccgta tttaataaaa atatttcgtg agtctctgtg cgctaaatat ccatctggcg
    18721 tagggaaaat cacacctgaa tcagaaatta acaatgaaac ctgtaaaacc acctcgtatt
    18781 aatggacgag tgccggtcct gtcggcacag gaagcggtga attatattcc cgacgaagca
    18841 acactttgtg tgttaggcgc tggcggcggt attctggaag ccacgacgtt aattactgct
    18901 cttgctgata aatataaaca gactcaaacg ccacgtaatt tatcgattat tagtccaaca
    18961 gggcttggcg atcgcgccga ccgtggtatt agtccgctgg cgcaagaagg tctggtgaaa
    19021 tgggcattat gtggtcactg gggacaatcg ccgcgtattt ctgaactcgc agaacaaaat
    19081 aaaattattg cttataacta tccacaaggt gtacttacac aaaccttacg cgccgccgca
    19141 gcccaccagc ctggtattat tagtgatatt ggcatcggga catttgtcga tccacgccag
    19201 caaggcggca aactgaatga agtcacgaaa gaagacctga ttaaactggt cgagtttgat
    19261 aacaaagaat atctctatta caaagcgatt gcgccagata tcgccttcat tcgcgctacc
    19321 acctgcgaca gtgaaggcta cgccactttt gaagatgagg tgatgtatct cgacgcattg
    19381 gttattgccc aggcggtgca caataacggc ggtattgtga tgatgcaggt gcagaaaatg
    19441 gttaagaaag ccacgctgca tcctaaatct gtccgtattc cgggttatct ggtggatatt
    19501 gtggtggtcg atccggatca aagccaactg tatggcggtg cgccggttaa ccgctttatt
    19561 tctggtgact tcacccttga tgacagcacc aaacttagcc tgcccctaaa ccaacgtaaa
    19621 ttagttgcga ggcgtgcatt attcgaaatg cgtaaaggcg cggtggggaa tgtcggcgtc
    19681 ggtattgctg acggtattgg cctggtcgcc cgggaagaag gttgtgctga tgactttatt
    19741 ctgacggtag aaacaggtcc gattggcgga attacttcac aggggatcgc ctttggcgcg
    19801 aacgtgaata cccgtgccat tctggatatg acgtcccagt ttaattttta tcacggtggt
    19861 ggtctggatg tttgttattt gagttttgct gaagtcgacc agcacggtaa cgtcggcgtg
    19921 cataaattca atggcaaaat catgggtacc ggtggattta ttgatatcag tgccacctcg
    19981 aagaaaatta ttttctgcgg cacgttaacc gcgggcagtt taaaaacaga aattgccgat
    20041 ggcaaattaa atatcgtcca ggaaggacgg gtgaagaaat ttattcagga actaccggaa
    20101 attactttta gcggaaaaat cgcgctcgaa cgggaactgg atgttcgtta catcaccgaa
    20161 cgtgctgtat tcacgctgaa agaagacggc ctgcatttaa tcgaaatcgc ccctggcgtc
    20221 gatttacaaa aagatattct cgacaaaatg gatttcaccc cagtgatttc gccagaactc
    20281 aaactgatgg acgaaagatt atttatcgat gcggcgatgg gttttgtcct gcctgaagcg
    20341 gctcattaac aggagtatca cgatggattt ttctttaact gaagaacaag aactgctgct
    20401 ggccagtatt cgcgaactga ttacgactaa ctttgcggaa gagtatttcc gcacctgcga
    20461 tcaaaacggg acatatccgc gtgagtttat gcgggcgctg gcggataacg gcatttctat
    20521 gcttggcgtg ccggaagagt tcggtggcat ccctgcggat tacgtcaccc agatgctggc
    20581 gctgatggaa gtgtcaaaat gcggtgctcc ggcatttttg attaccaacg gtcaatgtat
    20641 tcacagtatg cgccgtttcg gttctgcaga gcagctacgt aaaacggcag agagcaccct
    20701 ggaaacgggt gatcccgcct atgccctggc gttgacggag ccaggcgcag gctcagacaa
    20761 caacagtgcc actaccactt acacgcgtaa aaacggcaag gtttacatca acggacagaa
    20821 aacctttatt accggtgcga aagagtaccc gtatatgctg gtgttggcgc gcgatccgca
    20881 accgaaagat cccaaaaaag ccttcaccct gtggtgggtc gactccagta agcccggcat
    20941 taagattaac ccgctgcata aaatcggctg gcatatgctc agcacctgcg aagtctatct
    21001 cgacaacgtg gaggttgaag agagcgacat ggtgggcgaa gaaggaatgg gtttcctcaa
    21061 tgtgatgtac aactttgaga tggagcgcct gatcaacgcc gcgcgcagca ccggctttgc
    21121 cgaatgcgcc tttgaagatg ccgcccgcta tgccaaccaa cgtatcgctt ttggtaagcc
    21181 cattggtcat aaccagatga tccaggaaaa actggcgctg atggcgatta agatcgacaa
    21241 catgcgcaac atggtgctga aagtggcatg gcaagccgat cagcatcagt cactgcgcac
    21301 cagcgcggcg ctggcaaaac tgtattgcgc acgtaccgca atggaagtca ttgatgatgc
    21361 gattcaaatc atgggcggtc tgggctatac cgatgaagcg cgcgtctccc gcttctggcg
    21421 tgatgtccgt tgtgaacgta tcggcggcgg tacagacgaa attatgattt acgtagcagg
    21481 tcggcagatc ctgaaagact atcagaacaa ataatctgca ggcggcgcag cttcttaaca
    21541 aactgcgccg ccagatttat ccaacaagac ttaccggttg aggaaattca gataggattt
    21601 cttgttccgc aaaacagttt ttaaattgtc tacggtaatc gctgggcgaa caaccgacat
    21661 ggcgcaaaaa cagtttggca aagtgatcga cattttcata acccacacgc caggagatct
    21721 ctgcctgtga taattcagta ttagtgagtg accattttgc ttccgtcata cgccgttgaa
    21781 taacatagtt aataggggaa atacgatact ctttggtaaa ttcatggcaa atataactga
    21841 cgctggcacg aaattttttc gataactgtt ctaaagtgat tttttcgcga taattattac
    21901 tcagatagaa aagaatatct tttatcagaa catctttttt aatatacccc tgctccgaac
    21961 gataagcatt tttaaaattt tcgtagtaaa gaatggctaa cgtataggca aatgcatccc
    22021 ataacgaaga tgtttggcta tttttacttt gcggcaaaat cacacttagc tcattaaata
    22081 aggtttttat gacttctttt ccctgccccg cggcaattac cggacaagaa tgcggttgca
    22141 gtagctgatt ttcctcagcc ccctgaaact gaaagccgta cagcgcacag gtacacgtcg
    22201 ttgccggatc gttaacgtca gaggccaccg catgcagcct gccgcgttct atcaccacaa
    22261 tgtcatccgc atgtgcgaca tataacgaag aatcgatggt aaaccttgca acccctttct
    22321 tgacgtaaat caactcggtt tcattgtcat gaacgtgatg tccggactcc cattttggat
    22381 catcgctaaa tgcaaaacgt gaaagccgtg gcgttttacc ggcgacaaac agcgtttcac
    22441 tggcgttatc aaaacagcgt tgatacatga caacctccct attccatgag caagcaaaaa
    22501 caatatatgc cggataaaat acggcgtgta ttgcggttat acaaccgcgt ttagcgagag
    22561 ctaaacaaga ttatttacac tgaatgcaag attgtacggt catggaataa ctatattgcc
    22621 gcaaattgca cttttgtttt agcgatcgca tttttttgca agattgttgg caaggaaaac
    22681 agcttgctcc gtcgaaaacc cccaccgcta tcgcacacta ttttcaggcc atttttacct
    22741 tccatcggag atggttccgt atgcgactca caggagaaat catgaaaata ataacctgct
    22801 ttaagctggt gcctgaagaa caggacattg ttgtcactcc agaatacacc ctgaatttcg
    22861 acaatgccga cgccaaaatc agccagttcg atctcaatgc cattgaagct gcaagccagc
    22921 tcgcaacaga tgacgatgag atagccgcgc tgaccgttgg cggctcattg ttgcagaact
    22981 cgaaagtgcg cagtgcgcaa agatgtgcta tcccgcgggc cgcacagcct gtatttggtg
    23041 caggatgcgc aacttgaaca tgcactgcct ctcgataccg caaaagcgct ggcggcagca
    23101 attgaaaaga tcggcttcga tttactgatc tttggtgaag gttccggcga cctttatgcc
    23161 cagcaggttg gcttgctggt cggagaaatt ctgcaacttc cggtgattaa tgcagtgagt
    23221 gctattcagc gtcagggtaa tacactggtg attgaacgca cgcttgaaga tgatgttgaa
    23281 gttattgaac tctctgttcc agccgtgctc tgcgtcacct ccgatattaa cgtgccacgc
    23341 attccttcga tgaaagccat tctcggcgcg ggtaaaaaac cggtaaatca gtggcaggca
    23401 agtgatattg actggagcca gagcgcgcca cttgctgaac tggtcggcat tcgcgtaccg
    23461 ccgcaaacag aacgtaagca catcattatc gataacgatt cgccggaggc cattgccgag
    23521 ctggcggaac atctgaagaa agccctgaac tgaagcctac ggagaagaaa cgatgagtca
    23581 attaaacagc gtctgggtct ttagcgataa tcctgaacgt tatgctgaac tgtttggcgg
    23641 cgctcagcaa tggggccaac aggtgtatgc cattgtacaa aataccgacc aggcgcaggc
    23701 agttatgcct tatggtccaa aatgtattta tgttcttgag caaaacgacg cgctgcaacg
    23761 cactgaaaat tacgccgaaa gcattgctgc cccgctgaaa gataaacacc ccgctatgtt
    23821 gctgttggcc gcgacgaaac gtggtaaagc tctggcagca cggttaagtg tgcaactgaa
    23881 tgcggcgctg gtgaacgatg ccacggcggt ggatattgtc gatggtcaca tttgcgccga
    23941 acaccggatg tatggcgggt tagcgttcgc tcaggaaaag atcaacagcc cgctggcgat
    24001 cattaccctt gcacccggtg ttcaggaacc gtgcaccagt gatacctctc atcagtgccc
    24061 gacagaaacg gtaccttatg ttgctccgcg tcatgaaatt ctctgtcgcg aacgccgtgc
    24121 gaaagccgca agcagcgtgg acctgagcaa agcaaaacgt gtggttggcg tcggtcgtgg
    24181 tctggcggcg caggatgacc tgaaaatggt ccacgaactg gcggcggtgc tgaatgctga
    24241 agtcggctgt tcacgtccaa ttgccgaagg cgagaactgg atggagcgtg aacgttatat
    24301 cggtgtctcc ggcgtgttgc tgaaatccga tctctacctg acgctgggga tctccgggca
    24361 gatccagcat atggttggcg gcaacggcgc aaaagtgatt gtcgccatca ataaagataa
    24421 aaatgcgcca atcttcaatt atgccgacta cggtctggtg ggcgatatct acaaagtcgt
    24481 ccctgccctg attagccagt tgagccgcta attccccttt caaccacatc tcccgctgta
    24541 aacgcagcgg gaagggagca taacgcatgt cggatgacaa atttgatgcc attgtggtcg
    24601 gtgcggacgt tgctagtagc gttgccgcac tggtcatggc gcgagccgga ctggatgtcc
    24661 tggtgataga acgcggcgac agtgccggat gtaaaaacat gaccggcggg cgtctttatg
    24721 cccacacact tgaagcaatc attccaggct ttgcagtatc agcgccggta gaacgcaagg
    24781 tcacacgcga gaaaatctcc ttcttaaccg aagaaagcgc cgttaccctc gattttcacc
    24841 gcgagcaacc agatgttccg caacacgcat cttataccgt attgcgtaat cgtctggacc
    24901 cgtggttgat ggaacaagcc gagcaggcgg gcgcacagtt tatcccgggc gttcgcgtcg
    24961 atgcgttggt tcgtgaagga aacaaggtca ctggcgtgca ggccggggat gatattctcc
    25021 aagcgaatgt ggtggttcta gctgatggcg taaactcgat gcttggccgc tcgctgggaa
    25081 tggttcccgc ttccgatccg catcattacg ctgttggtgt taaagaggtt attggcctca
    25141 caccagaaca gatcaacgat cgctttaata ttacgggcga ggaaggtgcc gcctggctgt
    25201 ttgccggctc cccttctgac ggcctgatgg gcgggggatt tctctatacc aacaaggatt
    25261 ccatatcctt ggggctggtt tgtggattgg gtgatatcgc ccatgcgcaa aaaagcgtgc
    25321 cgcaaatgct ggaagatttt aaacaacacc ccgccattcg cccactaatt agcggcggca
    25381 aactgcttga atattccgcg catatggtgc cagaaggcgg tctggcaatg gtgccgcaga
    25441 tggttaacga tggcgtgatg atcgttggtg acgccgcagg cttctgcctg aatttgggtt
    25501 ttacagttcg tggcatggat ttagccattg catcggctca ggctgccgcc acaacggtga
    25561 tcgccgccaa agaacgcgag gatttctccg ccagcagtct ggcgcaatac aaacgtgagc
    25621 tggaacaaag ctgcgtcatg cgcgatatgc agcatttccg caagatcccg gcgctgatgg
    25681 aaaacccgcg cctgtttagc caatactcac gaatggtcgc cgacatcatg aacgagatgt
    25741 tcaccattga cggcaaacca aaccagccgg tacgcaaaat gatcatggga cacgcgaaga
    25801 aaattgggct gatcaacttg ctgaaagatg gcattaaggg agcaaccgcg ctatgagcca
    25861 gaacgctacg gttaacgtcg acatcaaatt aggcgtcaat aaattccatg ttgatgaggg
    25921 ccacccgcat atcattctgg cggcaaatcc cgatatcaat gaattccgta aattaatgaa
    25981 agcttgccct gccggacttt ataagcagga tgacgccggc aacattcatt tcgattccgc
    26041 cggttgtctg gagtgcggca cctgtcgggt gctgtgcggc aacactattc tcgaacagtg
    26101 gcaatatccc gcaggcacct tcggtattga ttttcgctac ggctaatcat gcatcccaca
    26161 ggcccgcatc tcgggcctga tgttctgttt cgagagtcca aaatgaaagt gacattaacg
    26221 ttcaacgaac aacgtcgtgc ggcgtatcgt cagcaagggt tatggggcga tgcttcactg
    26281 gccgattact ggcagcagac cgctcgtgcg atgccagaca aaattgccgt ggtcgacaat
    26341 catggtgcaa cgtacaccta tagcgcgctc gatcacgccg cgagctgtct ggcaaactgg
    26401 atgttagcga agggtattga atcaggcgat cgcatcgcat ttcaactgcc tggctggtgt
    26461 gaatttaccg ttatctatct tgcctgcctg aaaatcggtg cggtttccgt gccgctgttg
    26521 ccttcctggc gggaagcaga actggtgtcg gtgctcaata agtgtcaggc aaaaatgttc
    26581 tttgcaccga cgttgtttaa acaaacacgt ccggtagatt taatcctgcc gctgcaaaat
    26641 cagcttccac aactacaaca aattgtcggc gtggacaaac tggctcccgc cacctcttcc
    26701 ctctcattaa gtccgattat cgccgacaat acctcactga ccacggcgat aacgacccac
    26761 ggcgatgaat tagctgcggt gctgtttacc tccggaaccg agggtctgcc aaagggcgtg
    26821 atgctaacgc ataacaatat tctcgccagt gagcgggctt attgcgcgcg actgaatctg
    26881 acctggcagg atgtctttat gatgcctgcg ccacttggtc acgcaacggg ctttctgcat
    26941 ggcgtaacgg caccattctt aattggcgct cgcagcgtgt tgttagatat tttcactcct
    27001 gatgcgtgtc tcgcgctgct tgagcagcag cgttgcacct gtatgctagg cgcaacgccg
    27061 tttgtctatg atcttttgaa tgtactagag aaacaacccg cggacctttc agcgctgcgt
    27121 ttctttcttt gcggcggtac cacaatcccc aaaaaagtgg cacgtgaatg ccagcagcgc
    27181 ggcattaaat tattaagtgt ttatggttcc acagaaagtt cgccgcatgc ggtggtgaat
    27241 ctcgatgatc ctttgtcgcg ctttatgcac accgatggtt acgctgccgc aggtgtagag
    27301 attaaagtgg tcgatgacgc acgcaagacc ttaccgccag gttgcgaagg tgaagaagcc
    27361 tcgcgtggcc ccaatgtgtt tatggggtat tttgatgaac ctgaattaac cgcccgtgcg
    27421 ctggacgaag aaggctggta ttgcagcggc gatctctgcc gtatggatga ggctggctat
    27481 ataaaaatta ccggacgcaa aaaagatatt attgtccgcg gcggcgaaaa tattagcagc
    27541 cgtgaagtgg aagatatttt attgcagcat cctaaaattc acgatgcctg tgtggttgca
    27601 atgcccgatg aacgtttagg tgaacgatca tgcgcttatg tcgtgctgaa agcgccgcat
    27661 cattcattat cgctg
//
LOCUS       DAJVUJ010000039        27621 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.039, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000039 DAJVUJ010000000
VERSION     DAJVUJ010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27621
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.039"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(175..705)
                     /gene="ppa"
                     /locus_tag="OQQ48_001537"
     CDS             complement(175..705)
                     /gene="ppa"
                     /locus_tag="OQQ48_001537"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HCS1952351.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            1015..1971
                     /gene="ytfQ"
                     /locus_tag="OQQ48_001538"
     CDS             1015..1971
                     /gene="ytfQ"
                     /locus_tag="OQQ48_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HCS1952352.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENRIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            2111..3613
                     /locus_tag="OQQ48_001539"
                     /pseudo
     CDS             2111..3613
                     /locus_tag="OQQ48_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
     gene            3627..4649
                     /locus_tag="OQQ48_001540"
     CDS             3627..4649
                     /locus_tag="OQQ48_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1952353.1"
                     /translation="MPQSLPDTTTPKRRFRWPTGMPQLVALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     EVSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITSGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSRDKT"
     gene            4636..5631
                     /gene="yjfF"
                     /locus_tag="OQQ48_001541"
     CDS             4636..5631
                     /gene="yjfF"
                     /locus_tag="OQQ48_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HCS1952354.1"
                     /translation="MIKRNLPLMITIGVFVLGYFYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVVGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(5664..6662)
                     /gene="fbp"
                     /locus_tag="OQQ48_001542"
     CDS             complement(5664..6662)
                     /gene="fbp"
                     /locus_tag="OQQ48_001542"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HCS1952355.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            6838..8211
                     /gene="mpl"
                     /locus_tag="OQQ48_001543"
     CDS             6838..8211
                     /gene="mpl"
                     /locus_tag="OQQ48_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HCS1952356.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADTANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(8294..8845)
                     /locus_tag="OQQ48_001544"
     CDS             complement(8294..8845)
                     /locus_tag="OQQ48_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HCS1952357.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEE"
     gene            8939..10291
                     /gene="pmbA"
                     /locus_tag="OQQ48_001545"
     CDS             8939..10291
                     /gene="pmbA"
                     /locus_tag="OQQ48_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HCS1952358.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGVDCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            10475..10861
                     /gene="cybC"
                     /locus_tag="OQQ48_001546"
     CDS             10475..10861
                     /gene="cybC"
                     /locus_tag="OQQ48_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HCS1952359.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDTLKVVEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEGKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(10906..11370)
                     /gene="nrdG"
                     /locus_tag="OQQ48_001547"
     CDS             complement(10906..11370)
                     /gene="nrdG"
                     /locus_tag="OQQ48_001547"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HCS1952360.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVQRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(11560..13698)
                     /gene="nrdD"
                     /locus_tag="OQQ48_001548"
     CDS             complement(11560..13698)
                     /gene="nrdD"
                     /locus_tag="OQQ48_001548"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCS1952361.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYSNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(14092..15747)
                     /gene="treC"
                     /locus_tag="OQQ48_001549"
     CDS             complement(14092..15747)
                     /gene="treC"
                     /locus_tag="OQQ48_001549"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCS1952362.1"
                     /translation="MTNPPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAVLKKVCEFWADRGVDGLRLDVVNLISKDPRFPDDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(15797..17218)
                     /gene="treB"
                     /locus_tag="OQQ48_001550"
     CDS             complement(15797..17218)
                     /gene="treB"
                     /locus_tag="OQQ48_001550"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HCS1952363.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNARQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAETFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPNYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(17319..18284)
                     /gene="treR"
                     /locus_tag="OQQ48_001551"
     CDS             complement(17319..18284)
                     /gene="treR"
                     /locus_tag="OQQ48_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HCS1952364.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPEFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLSRSFPER"
     gene            18469..18522
                     /gene="mgtL"
                     /locus_tag="OQQ48_001552"
     CDS             18469..18522
                     /gene="mgtL"
                     /locus_tag="OQQ48_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HCS1952365.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            18663..21359
                     /gene="mgtA"
                     /locus_tag="OQQ48_001553"
     CDS             18663..21359
                     /gene="mgtA"
                     /locus_tag="OQQ48_001553"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HCS1952366.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTVVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQLSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(21565..21951)
                     /gene="ridA"
                     /locus_tag="OQQ48_001554"
     CDS             complement(21565..21951)
                     /gene="ridA"
                     /locus_tag="OQQ48_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HCS1952367.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAIRR"
     gene            complement(22024..22485)
                     /gene="pyrI"
                     /locus_tag="OQQ48_001555"
     CDS             complement(22024..22485)
                     /gene="pyrI"
                     /locus_tag="OQQ48_001555"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HCS1952368.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(22498..23433)
                     /gene="pyrB"
                     /locus_tag="OQQ48_001556"
     CDS             complement(22498..23433)
                     /gene="pyrB"
                     /locus_tag="OQQ48_001556"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HCS1952369.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQSELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(23437..23571)
                     /gene="pyrL"
                     /locus_tag="OQQ48_001557"
     CDS             complement(23437..23571)
                     /gene="pyrL"
                     /locus_tag="OQQ48_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HCS1952370.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            complement(23851..24246)
                     /locus_tag="OQQ48_001558"
     CDS             complement(23851..24246)
                     /locus_tag="OQQ48_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCS1952371.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     SYPTWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(24377..25090)
                     /gene="bdcA"
                     /locus_tag="OQQ48_001559"
     CDS             complement(24377..25090)
                     /gene="bdcA"
                     /locus_tag="OQQ48_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCS1952372.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADKDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMSHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            25161..25754
                     /locus_tag="OQQ48_001560"
     CDS             25161..25754
                     /locus_tag="OQQ48_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCS1952373.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGNKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLAEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSYDPQARDIAVQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTILKE"
     gene            25899..26351
                     /gene="tabA"
                     /locus_tag="OQQ48_001561"
     CDS             25899..26351
                     /gene="tabA"
                     /locus_tag="OQQ48_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HCS1952374.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPHEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPARVRKAVVKMLMA"
     gene            26474..27621
                     /locus_tag="OQQ48_001562"
                     /pseudo
     CDS             26474..27621
                     /locus_tag="OQQ48_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234052.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
BASE COUNT         6624 a         6841 c         7304 g         6852 t
ORIGIN      
        1 ttccagcaag tggctatgaa cagcaaaatg gtggtcgggt ttgcttgttc ttaccgttca
       61 agccttgcaa ccgctgagaa taatgatggt ggaatagaga gtttatttat cgcgggcata
      121 aaaaaaccct tactaaccga agcccggcgt tcagggttat tgcgctagaa gaacttattt
      181 attctttgcg cgctcgaagg aagcaacgat ttcagcttta gcggcttctg cgttttccca
      241 accttcaact ttcacccact tgcctttttc gaggtctttg tagtgctcga agaagtgagc
      301 gatttgcgct ttcagcagtt caggcagatc gtttacgtct ttaatgtgat cgtattcttt
      361 gctcagcttg ctgtgcggaa ccgcaaccag tttcgcatct tcaccggctt cgtcggtcat
      421 tttcagaacg ccaaccggac ggcaacggat cacagaacca ggctgcagcg ggtacggagt
      481 cgggaccagt acgtcaaccg ggtcaccgtc cagagacagg gtgtggttga tgtaaccgta
      541 gttgcacgga tagaacatcg cggtggacat gaagcggtca acgaacagtg cgccgctctc
      601 tttgtcgatt tcgtatttga tcggatctgc gttagccggg atctcaataa caacgtagat
      661 gtcttccggc agatctttac ccgcagggac gttgagtaag ctcatgtctg tttcctttaa
      721 aaatatgtgg caaacaagtg ccgagtatta tagccaactc gcgccgaatg tctttgcttg
      781 ttttcgtctt cattttcctt tttcgccagt tttaagacgg tatacagagc aggaaaatcc
      841 ataaccgtag ccgcattttt catagtgaga tgaaagcgat tacaaacttg tgattaacgt
      901 tttatttact tttttgaagt gtgatgtaac gcaatccgtt acataacgaa ttgtctatag
      961 ttttttcgcg aacatctttt caccaataat aattaccccg acgaggacaa ccctatgtgg
     1021 aaacgcttac ttgtagtctc tgcagtctcg gcagccatgt cgtctatggc gttggccgct
     1081 ccattaaccg taggattttc gcaggtcgga tcggaatccg gctggcgcgc cgcagaaacc
     1141 aatgtggcga aaagtgaggc cgaaaaacgc ggaattacgt tgaaaattgc cgatggtcag
     1201 caaaagcagg aaaaccggat taaagcggta cgttccttcg ttgcacaagg ggtggatgcg
     1261 atctttatcg ctccagtggt tgcgacgggt tgggaaccgg tattaaaaga ggcgaaagat
     1321 gccgaaatcc cggtcttctt gctcgaccgt tccatcgatg tgaaagacaa atctctctat
     1381 atgaccaccg ttactgccga caacatcctc gaaggtaagt tgatcggtga ctggctggta
     1441 aaagaagtga atggcaaacc atgcaacgtg gtggagctgc agggcaccgt tggggccagc
     1501 gtcgccattg accgtaagaa aggctttgcc gaagccatta agaatgcgcc aaatatcaaa
     1561 attatccgct cgcagtcagg tgacttcacc cgcagtaaag gcaaagaagt catggagagc
     1621 tttatcaaag cggaaaacaa cggcaaaaac atctgcatgg tttacgccca taacgacgac
     1681 atggtgattg gtgcaattca ggcaattaaa gaagcgggcc tgaaaccggg caaagatatt
     1741 ctgacaggtt ctatcgatgg cgtaccggat atctacaaag cgatgattga tggcgaagcg
     1801 aacgccagtg ttgaactgac gccgaatatg gcgggacccg cttttgacgc gctggagaaa
     1861 tacaaaaaag acggcaccat gcctgaaaag ctgacgttaa ccaaatccac cctttacctg
     1921 cctgataccg caaaagaaga gttagagaag aagaaaaata tggggtattg agggttgcta
     1981 tgcctgatgc cgattcgtag gccggataag gcgctcgcgc cgcatccggc gatggtgcac
     2041 tgaagcctga tgcgacgctt accgcgtctt atcatgccta ctgggagcac gctttacacc
     2101 gggggaaacc atgacgaccg accaacacca ggaaatcctc cgcaccgaag gattaagtaa
     2161 atttttcccc ggcgtcaaag cgttagacaa cgttgatttc agcctgcgcc gtggcgaaat
     2221 catggcgctg ctcggtgaaa acggggcggg aaaatcaacg ctaatcaaag cattaactgg
     2281 tgtataccac gccgatcgcg gcaccatctg gctggaaggc caggttatct caccgaaaaa
     2341 taccgcccac gcgcaacaac tcggcattgg caccgtctat caggaagtca acctgctacc
     2401 caatatgtcg gtcgctgata atctatttat aggccgcgaa cccaaacgct tcggccttct
     2461 acgccgcaaa gagatggaaa agcgcgccac cgaactgatg gcatcttacg gtttctccct
     2521 cgacgtgcgc gaaccgctca accgcttttc agtcgcgatg cagcaaatcg tcgccatttg
     2581 ccgggctatc gatctctccg ccaaagtgct gatcctcgat gaacccaccg ccagcctcga
     2641 cacctaggaa gtggagctat tatttggcct gatgcgtcag ttgcgcgatc gcggcgtcag
     2701 cctgatcttt gtcactcact ttctcgatca gagctatcag gtcagcgatc ggatcaccgt
     2761 tttacgcaac ggcagtttcg taggctgtcg ggaaacgcgc gagctaccgc agatcgaact
     2821 ggtaaaaatg atgctggggc gcgagctgga tacccacgcg ctacagcgtg ccgggcgaac
     2881 attgttgagc gacaaacccg ttgccgcgtt caaaaattac ggcaaaaaag gaacgatagc
     2941 accgtttgat ctcgaagtgc gccccggcga gatcgtcggc ctggcaggct tgctgggatc
     3001 cggacgtacc gaaaccgccg aagtgatctt cggtatcaaa cctgctgaca gcggcacggc
     3061 gttgatcaaa ggcaaaccgc aaaacctgcg atcgccacat caggcttcgg tactgggcat
     3121 cggcttctgc ccggaagaca ggaaaaccga tggcatcatc gctgccgcct cggtgcggga
     3181 aaatatcatc ctcgctctcc aggcccagcg cggctggcta cgacctattt cccgcaaaga
     3241 gcagcaagag attgccgaac gctttatccg ccagcttggc attcgcacac cttcaactga
     3301 acaaccgatt gaatttctct ccggcggcaa tcagcaaaaa gtgttgcttt cacgttggct
     3361 actgacccga ccgcaatttc tgatcctcga tgagccaacc cgtggcattg atgttggtgc
     3421 ccacgccgag atcatccgcc tgattgaaac gctatgcgcc gatggtctgg cgctgctggt
     3481 gatctcctcc gaactggaag aactggtggg ctatgccgac cgggtgatca tcatgcgcga
     3541 tcgcaaacag gtggcggaga tccggctggc agagctttcc gttccggcta tcatgaacgc
     3601 cattgcggcg taaggagaac agtgtgatgc ctcaatctct tccggacacc actacgccaa
     3661 aaaggcgctt tcgctggccg acggggatgc cgcagctggt agcactattg ctggtgctgc
     3721 tggtcgacag cctggtagcc ccgcatttct ggcaggtagt gcttcaggat gggcgtttgt
     3781 tcggtagccc catagacatt cttaaccgtg cagcccccgt tgcgttactg gcgattggta
     3841 tgacgctggt gatcgccacc ggtgggattg atctctccgt aggggcggtg atggctatcg
     3901 ccggagccac aacggctgcg atgacggtcg cggaagtcag cctgccgatt gttttgttaa
     3961 gcgccctagg caccggcatc ctggcgggat tgtggaacgg catactggta gcgatcctca
     4021 aaattcagcc atttgtcgcc accctgatcc tgatggtcgc cgggcgcggc gtggcgcaac
     4081 tgatcacctc cggacagatc gtcacgttta actcgccgga tctctcatgg tttggcagtg
     4141 gatcgctgtt gttcctgcca acgccagtca ttattgcggt actgacgctt ctcctgttct
     4201 ggctgttgac ccgcaaaacg gcactgggga tgtttatcga agccgttggt atcaacattc
     4261 gggcggcaaa aaatgccggg gtaaacacgc ggatcatcgt catgctcact tatgtgttga
     4321 gcgggctgtg tgcggcgatt gcgggcatta tcgtggcggc ggatattcgc ggtgccgatg
     4381 ccaacaacgc cggattatgg ctggagctgg acgccattct cgcggtggtg attggcggcg
     4441 gatcgctgat gggcgggcgc tttaacctgc tgctctcggt agtgggggcg ctgattattc
     4501 aggggatgaa caccggaatt ttgctttcgg gctttccacc ggagatgaac caggtggtga
     4561 aagcggtggt ggtgctttgc gtgctgattg tccagtcgca acgctttatc agtctgatta
     4621 aaggagtacg tagccgtgat aaaacgtaat ttgccactga tgatcaccat cggcgtcttt
     4681 gtgctgggct atttctactg cctgacgcag tttcccggtt ttgcttccac aagagtgatc
     4741 tgcaatatcc tgaccgataa cgcttttctg gggattattg ccgttggtat gacctttgtg
     4801 atcctctctg gcggtatcga tctctccgtc ggttcggtga tcgcctttac tggcgtgttt
     4861 ctggcaaaag tggttggcga tttcggcctc tcgccgctgc tggcgtttcc gctagtgctg
     4921 gtgatgggct gtgccttcgg cgcatttatg gggcttctga tcgacgccct gaagatcccg
     4981 gcatttatca ttacgcttgc ggggatgttc tttttgcgcg gcgtcagcta tctcgtttcg
     5041 gaagagtcga ttccgataaa ccatcccatt tatgacacgc tctcaagcct tgcgtggaaa
     5101 atccccggcg gcggtcgcct tagtgcgatg gggctgttga tgctggcggt ggtggttatc
     5161 ggtattttcc ttgcgcatcg tacccgtttt ggcaatcagg tgtacgctat tggcggcaac
     5221 gcgacgtcgg cgaacctgat ggggatttct actcgcagca ctactattcg catttatatg
     5281 ctctccaccg gactggcaac actggcgggg attgtcttct cgatttatac ccaggccgga
     5341 tatgcactgg cgggcgtagg tgtggaactg gacgccatcg cgtcggtggt gattggcggt
     5401 acgttgttaa gtggtggcgt cgggacggtg cttgggacgc tttttggcgt ggcaattcag
     5461 ggactgatcc agacttacat caactttgac ggcacgctga gttcctggtg gacgaaaatc
     5521 gccatcggta ttttgttgtt tatttttata gcattacagc gtgggttaac ggtgctgtgg
     5581 gagaatcgtc agagttcgcc agtgacaaga gtcaacattg cgcagcgata aaaatgcctc
     5641 tccgtgtgga gaggcgcagg agattacgcg tccgggaact cacggataaa gcgttcgaca
     5701 tcttcaacca tatggtcgtt gccgacaaag aacgaacggc gctggtgcag agtttccggg
     5761 atgatatcca gaatacgctc tttgccatcg ctcgctttac cgcccgcttg ttccgccagg
     5821 aatgccatcg ggttgcactc ataaagcaaa cgcagtttgc cgtccgggtg gctggcggtg
     5881 cttgggtaga gataaatacc gcctttcagc aggttacggt ggaaatccgc aaccagtgaa
     5941 ccgatataac gtgaggtata cgggcggttg gtggatttat cttcttcctg gcagaattta
     6001 atgtacttct tcaccccgtt cggaaactta atgtagtttc cttcgttgat ggagtaggtt
     6061 ttgcctttct ccgggaagcg catccgttcc tggcacaggc agaaaacgcc cagcgaagga
     6121 tcgtaagtaa aggcgtgaac accgcatccg gtggtgtaaa ccagcatggt agaggagccg
     6181 tataccacgt aacctgccgc aacctgtttg ttaccaggct ggaggaaatc ttcttccgtt
     6241 accggcgtgc cgacgggcgt aactcggcgg tagatggaga aaatggtacc gacagagacg
     6301 ttaacatcga tgttggacga gccatccagt gggtccatca gcaccacgta ttttgcatgt
     6361 tcacagcctt caaagacaac aatctcatct tcttcttcag aggcaatgcc tgcaacgata
     6421 tcgcgtgctt tcagtgcggc tttcagtttt tcattagcga acaagtcgag tttttgctga
     6481 acctcgccct gcacgttctc agcaccgctg gcacccagga tatcaaccag tcctgctttg
     6541 ttgatatcgc gatggataat cttggcgccc agttttattg ccgacagcaa agcagtgagc
     6601 tcaccggtag catgagaaaa ctcgtgctgc ttttcgacaa taaattcacc taacgttttc
     6661 ataaaacttt ccctgcaatg tttatggagt aaagcgaccg caacaatctt aacaaataat
     6721 ctcaatgttg cgctcaggtg aatcgcgcca gcaaattacg gattatcctg aaatgcgttt
     6781 ctcacttgcc cgacatatgc gtaaaatgag cggcagatta aaaaaggata gtgacgtatg
     6841 cgcattcata ttttaggaat ttgtggcacg tttatgggcg gtctggcgat gctggcgcgc
     6901 cagttaggcc atgaagtaac gggttcggac gccaatgtgt atccgccgat gagcacctta
     6961 cttgagaagc aaggcattga actgattcag ggttacgatg ccagccagct cgatccgcag
     7021 ccggatctgg tgattattgg caacgccatg acccgtggaa atccgtgtgt ggaagcggta
     7081 ctggaaaaaa acatccctta tatgtcaggt ccacagtggc tgcacgattt tgtgctgcgc
     7141 gaccgctggg tgctggccgt tgccggtaca cacggcaaaa ccaccaccgc gggaatggcg
     7201 acctggattc tggaacagtg tggttacaaa ccgggatttg tgatcggcgg tgtgccgggg
     7261 aactttgagg tttcggcgcg tctgggcgaa agcgacttct ttgttatcga agcggatgag
     7321 tacgactgcg ccttcttcga caaacgctct aaatttgtcc attactgccc gcgtacgctg
     7381 atcctcaaca accttgagtt cgatcacgcc gatatctttg acgacctgaa agcgatccag
     7441 aaacagttcc accatctggt gcgtatcgtt ccggggcagg gccgtattat ctggccggaa
     7501 aatgacatca acctgaaaca gaccatggcg atgggctgct ggagcgagca ggagctggtg
     7561 ggtgagcagg ggcactggca ggcgaaaaag ctgaccactg atgcttccga atgggaagtc
     7621 ttgctggatg gcgaaaaagt gggcgaagtg aaatggtcgc tggtaggcga acataatatg
     7681 cataatggcc tgatggcgat tgcggcggcc cgccatgttg gcgtagcgcc ggcagatacc
     7741 gctaacgcgc tgggttcgtt tattaatgct cgtcgccgtc tggagttgcg tggtgaagcg
     7801 aatggcgtca cggtatatga cgattttgcc catcacccga cggcgattct ggcaacgctg
     7861 gcggcgctgc gtggcaaagt tggtggtaca gcgcgcatta ttgctgtgct ggaaccgcgc
     7921 tcgaatacca tgaaaatggg gatctgcaaa gacgatttgg caccttcatt aggtcgtgcc
     7981 gatgaagtct tcctgctgca accggcgcat attccgtggc aggtggcaga agtggcagaa
     8041 gcctgcgttc agcctgcaca ctggagtggc gatgtggata cgctggcaga tatggtggtg
     8101 aaaaccgctc agcctggcga ccatattctg gtgatgagca acggcggttt tggtgggatc
     8161 catcagaaac tgctggatgg tctggcgaag aaggcggaag ctgcgcagta attcggcctc
     8221 agcctgagat agcattgccg gataaggcgt ttacgccgca tccggcaaac catactcaca
     8281 tcaacaacga aaattactct tcattctccg ccagttcgcg cagatactgg aaaatctggc
     8341 gtgcggattt cggcggctta ttcccttctt tctctttctt cgcgttgcgg atcagagtac
     8401 gcagttgctg acgatcggca tcgggccaca gatttaatac ctcagcgatg gcgtcatcac
     8461 cttgatcgat cagacgatcg cgcaggtttt ccagtttatg gaacagcacc acctgctggt
     8521 tgtgacggtt tttcagctta tccagcgcct gacggatcgg ttctacatcg cgctggcgca
     8581 gcatcttacc gatgagctgc aactggcggc ggcgaccttc catcttaata cgctgggcga
     8641 gctcaatagc cgcgcgcaga tccgcatcta acgggatctt atccagcgcg tttttcccca
     8701 gatcaacaat ttccgcgcca aggcgtttta gctcctcggc atcacgttta atttcacttt
     8761 tactgaccca gataatttca tcgtcttcgt cttcgatgtc atcaccggga acgtcgtcga
     8821 gccagtcttc gggctgctta gtcatctcag gctccttaaa aaaagaggct aatgttacca
     8881 gttaagatgc gcactgaaaa acggttctct gttagacttc agagaaactc tctacattat
     8941 ggcacttgca atgaaagtaa tctctcaagt tgaagcgcag cgcaagattc tggaagaagc
     9001 agtttccact gcgctggagt tggcctcagg caaatcggac ggtgcggaag ttgccgtcag
     9061 caagaccacc ggcattagcg taagcacgcg ttatggtgaa gtggagaatg ttgaattcaa
     9121 tagcgatggc gcgctgggga tcactgttta tcaccagaac cgcaaaggta gcgcatcatc
     9181 caccgattta agcccgcagg ccattgcccg tactgtacag gcggcgctgg atattgcccg
     9241 ttatacctcg ccagatccct gtgccggcgt ggcagacaaa gagctgctgg cctttgacgc
     9301 accagatctc gacttgttcc accctgcgga agtttccccg gatgaagcca ttgaactggc
     9361 ggcccgcgca gaacaggcgg cattgcaggc ggacaaacgc atcaccaata ccgaaggtgg
     9421 cagctttaac agccactacg gtgtcaaagt ttttggcaac agccacggca tgttgcaggg
     9481 ttactgctca acgcgtcatt cgctctccag ctgtgtaatt gccgaagaaa atggcgatat
     9541 ggagcgtgat tacgcctaca ccattggtcg tgcgatgagc gatctgcaaa cgccagagtg
     9601 ggttggggtc gactgtgctc gccgtacttt atcgcgcctg tcaccgcgta aactctccac
     9661 catgaaagcg ccggtcattt ttgccaatga agtggcaacc gggctttttg gccatctggt
     9721 gggggcgata gcgggtggat cggtttaccg taaatctacc ttcctgctgg attcgctggg
     9781 taaacaaatt ctgccggact ggctgaccat tgaagagcat ccgcatctgc tgaaagggct
     9841 ggcgtcgacg ccattcgaca gcgaaggtgt gcgcaccgag cgtcgcgata ttattaaaga
     9901 tggcatcctg actcagtggc tgctgaccag ctattcggcg cgaaaactgg ggctgaaaag
     9961 caccggacat gcgggcggta ttcacaactg gcggattgcc ggacaaggtc taagcttcga
    10021 gcagatgctc aaagagatgg gcaccgggct ggtggtgacg gaattgatgg gccagggcgt
    10081 gagtgcaatt accggtgatt attcccgtgg tgcggcgggc ttctgggtag aaaacggcga
    10141 aattcagtat ccggttagcg aaatcaccat cgcaggtaat ttaaaagata tgtggcgcaa
    10201 tattgtcacc gtcggtaacg atattgaaac acgcagtaat atacagtgtg gttctgtgct
    10261 gttgccggag atgaaaatcg ccggacagta aaaaaactgg cgcgacctgt cgcgctataa
    10321 actaaaaaat tacacaaaat cattcgcact gcatcgagac ggaaagtgaa tgagggccgg
    10381 ggagcgtact cgcagtacgt gacccggtcg aatgagcgta gtcgtcgatg aggcagtgtg
    10441 aatgatgaag tgtaaataaa aaaggaagtg agcaatgcga aaaagcctgt tagctattct
    10501 tgcagtctcc tcgttggtat tcagttcggc gtcgtttgcc gctgatcttg aagacaatat
    10561 ggaaaccctc aacgacactt taaaagtggt cgaaaaagcc gataacgcgg cgcaagtcaa
    10621 agacgcgtta acgaagatgc gcgccgcagc gctggatgcg caaaaagcaa cgccgccgaa
    10681 gctcgaaggc aaatcaccgg acagcccgga gatgaaagac ttccgtcacg gtttcgatat
    10741 tctggtcggt cagattgacg acgcgctgaa gctggcaaat gaaggtaaag taaaagaagc
    10801 gcaggctgcc gcagagcaac tgaaaacgac ccgcaacgcc tatcaccaga agtatcgtta
    10861 attcctcatt tccctgttgc ctgcactcag gtaacaggga acttatcatc gcaaatgatg
    10921 caccacctga ttgctgctgc cgcgccagat cagggaaggg tcttttaaat cctgcacaaa
    10981 tttgccgtcg accagcacgt taatcaaatc aacaacctgc atttgcgctg cgttgagttc
    11041 gtcgagttta tagcctgtcc acacccagat gtctttaccc ggacactcgg cgcggatgcg
    11101 ttgtaccagt ttcagaatat ccggcacgtt ttgcggatgc agcggatcgc cgccggagag
    11161 ggaaataccc tggcgtttga tacgagtgtc attcagatcg ttaatgatct ggtcttccat
    11221 tgctttagta aatggctgac cggaatttac ccgccatgtg cttttgttat agcaaccggg
    11281 gcattcatga acacacccgg agacaaacag agtgcaacga gtgccggggc cgttgacgat
    11341 gtcgacagga tagtactgat gataattcat tatttcgcct ggcttatcag tccgcgtgta
    11401 gactgcatcc ggtaatcaat gcctgatgcg acgctgtcgc gtcttatcag gcctacagcc
    11461 gttgccaaat gtaggccgga taaggcgttt acgccgcatc cggcaatcaa cgcgggatgc
    11521 gacgctttca catcacatcc ggcatttgct tagtgcagat caacctatct gcccatttcc
    11581 caaatgttta acgcggcgct taacttcttc ctgcttgccg gcgttaaacg gacgtgcatc
    11641 cgggctacct aaatatccgc acacgcggcg agttaccgac acacgggagg cgtcatggtt
    11701 accacatttc gggcaggtga agcctttgct ggtgcactcg aactcaccgg taaagccaca
    11761 ctcgtagcac tcatcaatcg gtgtattggt gccgtaatac ggtacatgct gatagctgta
    11821 atcccagaca tcttccagcg ccttcaggtt gtgctgaatg tttgaatact cgccgtagca
    11881 aatgaaacca ccgttcgcca gcggcgggta aggcgcttca aagtcgatct tgtcgtacgg
    11941 gttcaccttc ttctccacat cgaggtggaa actgttggtg tagtaacctt tatcggtcac
    12001 gcccggcacc acgccaaact cagcagtatc gagacggcag aagcggtcgc acaggttttc
    12061 actcggcgtg ctgtagagac tgaaaccata acccgtttct tccttccact gatccactgc
    12121 ctgacgcaga cgttcaacaa tggcgatacc tttcgcgcga agctgctcgt tgtcgtaaac
    12181 atgctcgccg ccgaacagcg cgttaatggt ttcgtggatg ccgatgtaac ccagcgaaat
    12241 agacgcacga ccgtttttga agatttcaga aacatcatcg tcagcattga gacgcacgcc
    12301 acaagcacct tccatataga ggatcggggc cacgcgcgct ttcacgcctt cgagacgagc
    12361 gatacgggtc atcagcgcct tacgtgccag caccagacgt tcatccagca gcttccagaa
    12421 ggtggcttca tcgccttttg cttccagagc aatacgcggc aggttcaggc tgatcacgcc
    12481 gaggttgtta cgaccatcgt ggatctgctc gccgttttca ttttcccaca cgccgaggaa
    12541 gctgcggcag cccatcggag ttttaaacga accggtgact ttcactacct gatcgtagtt
    12601 caggatatcc ggatacatgc gcttgcttgc gcactccagc gccagctgtt tgatgtcgta
    12661 gttcggatcg ccttttttat ggttcaggcc atcgcgaatc gcaaacacca gtttcgggaa
    12721 caccgcagtt ttacggtttt taccgaggcc tgcgatacgg ttacgcagga tagattcctg
    12781 aatcaggcgc gactcccagc tggtgcccag accaaaacca aaggttacaa acggcgtctg
    12841 accgttggcg gtgtgcaggg tgtttacttc gtactccagc gactggaagg catcgtagca
    12901 ctctttaatg gtacgagagt tagcgtagcc ttcggcgtcc gggatgttcc actcttctgc
    12961 ggtcttgcga tgtttgttgt agctggcggt gacaaacggt gccagcactt catcgatacg
    13021 gttaatggtg gtgccgccat aaatatggct ggcgacctga gcaataatct gcgcagttac
    13081 agcggttgcc gtagagatcg acttcggcgg ttcaatctcg gcgttcccca tcttaaagcc
    13141 ctgggtcagc atgcctttca ggtcgatcaa catgcagttg aacatcggga agaacggcga
    13201 gtaatcgaga tcgtgatagt gaatatcgcc acgctcatgt gcctgcacca cgtcacgcgg
    13261 cagcaggtgc tgacgtgcat agtgtttagc cacgatcccg gccagcaggt cgcgctgggt
    13321 tggaatcacc ttgctgtctt tgttggcgtt ttcgttgagt aacgaggcgt tggtctgctc
    13381 gaccagacca cggatctcct gattcaggcg accgcgtttt tcacgttcaa tgtcgcgatc
    13441 gtgacggtac tcgatgtaag cacgagccag ttgtttgtat ggacctgaca tcagctggtt
    13501 ttcaacagcg gtctggatct cattgatatc cacctggttg cggccctgca tctgctcgct
    13561 gacaactgca gcaacagtgg cgcaataatc ggcatcatcg acttccgctg ctttagctgc
    13621 acgcagaatc gcttctttga tgcgctctga tttaaacggc actttgcagc cgtctcgttt
    13681 catcacatgc ggtgtcatga tcgctccata tttttaagaa caggttatcc acagaaattg
    13741 ggaaaggcgt tttccggttt tttcgtttcg ttttccggtg ctttccgcaa tcgccatccg
    13801 cttttccaca acgccgcacc tttttcgtgg ggagctgtag tagcaattat agtcgattaa
    13861 tacaacatat tgggttggga cgcattttaa agtctatata tagtgctttg catcaaggat
    13921 gtttgaactt tttttgatgt agctcaaagt aaaaagcaga gcgtacggat gacgggcgct
    13981 acagcgttat gtaaattttt taatgaattt gctggttgaa aaataaacaa aaacaacatg
    14041 ctgacagaca aaaccccgga atgaccggca ttaccggggc ttagggaaga tttacttctg
    14101 taaccaccag acagcctcaa aaggccgtaa attcatggca cagggttgtg gtgaggcttc
    14161 ttcgtagtta tgcatcacaa gctgccagtt gccgcgcatt tgccctggct gccagggttg
    14221 gatctcacgg ctaaggttgg caatgaccag caaggtttgc cccttccatt cacggcgata
    14281 gcaccacaat acagggctgt ttggcagcag atcctggtaa ttgccccatg tcaggatggc
    14341 ttcctgctta cgcagtgcga ttaacttttg gtaggtgtaa aacaccgagg aatcatcggc
    14401 cagcgcggct tctacgttga tttgttgata gttatcaccc aggccaatcc acggttcgcc
    14461 agccgtaaat ccggcattat cgccgttgct ccattgcatg ggcgtgcggc tgttgtcacg
    14521 ggatttactg gcgaggattg ccaataactc gtcggcatca cgaccatcgt tgcgcagctc
    14581 ggcaaacata ttgaggctct ctacgtcgcg atagtcagta atgcgcgtga aatgcgggtt
    14641 ggtcatgcca atctcttcgc cctggtagat atacggcgtt ccctgcatgc catgcagcac
    14701 catcgccagc atttttgccg caggcacgcg gtattcacct tcatcaccaa agcgagaaac
    14761 aatgcgcggc tgatcgtggt tacaccagaa caaggcattc catgctacgt tgtgcattcc
    14821 ttgttgccag tggcggaaca atgttttcaa cgccacaaag tcaggtttag ccagcgtcca
    14881 tttttcacca ccgggataat cgaccttcag gtgatgaaaa ttaaaggtca tcgacaattc
    14941 actgcctgtc agtgccgcgt atcgctggca atgctcaagg ctggtggagg acatttcacc
    15001 tacggtcatt aacccgcgtg gcgtaaacac atcgcggttc atctcgtgca aaaactcgtg
    15061 tgctcgtggc ccgtcggtgt agaagcgacg cccgtcgcca tccaggtcat cagggaaacg
    15121 cgggtctttg gagatcagat tcaccacatc caggcgcaac ccgtcgaccc cacgatcggc
    15181 ccagaactca cagacttttt tcagcactgc gcgtaccgct ggattctccc agttgagatc
    15241 cgcctgttct ggtgcaaaga gatgcaaata gtactgttcg ctttccgcat gccagcgcca
    15301 cgcactaccg ccaaattttg aacgccagtt gttcggtggc gtttctggtt ctccatcgcg
    15361 ccagatataa aactggcggt aagggctttc tttgttcagc gcctcgcgaa accaggcatg
    15421 ttgggtagag gtatggttaa acaccatatc gagaatgata cgaatcccgc gcgattttgc
    15481 ctgcgtcacc agttcgtcaa aatcgtccag cgtgccgtag gtgggatcaa tcgccgtata
    15541 gttcgctacg tcgtaaccgt tatcgacctg gggagagaca taaaaggggg ttagccagat
    15601 ggcatcaacg cccagtttat gcagatagtc caggcgttgg ataacgccac gtaaatcgcc
    15661 ggtaccgcta cccgtggtgt cctgaaaact ctttggataa atctggtaga taacgccgtt
    15721 ttgccaccag tggggaggat tagtcattac gttattcctg caaatgcgag ggggcgcaat
    15781 tgcgccccga agaaaattaa acaatgtcca gcgtgcccag gcggtatttc cgctgataga
    15841 taaacgaggt gagtacaatc gggatgatga tggcgatagc cattgccagc gcaaacacct
    15901 gccagtagtt cggttgaatc gagagaattc ccggcaggcc gcctacgccg atgccattcg
    15961 ccataacgcc gttcaggccg catagcaatc ctgccagacc agaaccaatc atcgcgcaca
    16021 gcatcgggaa gcgatatttc aggttgatgc cgtacattgc aggctcagtg accccaagcc
    16081 aggcggagat cgccgcaggc acggagatct cgcgttcatt gtgcttgcgg ctggaaatga
    16141 taatgccgat cacggcggag ccctgagcga tattcgacag cgcaatcagt ggccacactg
    16201 gcgtaccacc catgctttga atcatctgca aatcaatagc aagcgtggtc tggtgtaccc
    16261 cggtgatcac cagcggggcg tacaggaagc caaacaatgc cgcgccaatc ggagcaaagc
    16321 tgccggtcat caggtgacgt accgcaaagg caacgccatc gccaatcatg cgaccaaacg
    16381 gaccaatcag cgcatgggcg aggaacaccg cgaggatcag cgaacagacg ggcaccacca
    16441 ccagatagag gtaatctggc acgatgcgtt taaggcgagt ttcaataacg cccagcgcca
    16501 gcccggctaa cagtgccggg atcacctgcg cctgatagcc cactttggcg atgctgaaca
    16561 tgccaaagtc ccacacttcc ggcagctgct gcccgagcag ataagcgttc atcagttggg
    16621 gggaaaccag tgtcacgcca agcacgatac caaggatcgg tgtgccgccc atttttttca
    16681 ccgctgacca gcaaatcccg accggcaggt agaagaagat cgcttcaccg atcaaccaca
    16741 gaaaatcgta gatcgtttgc agggaagggt acatttgcgc cagcgtttga ccgttgctca
    16801 tgggcaaatc gccgatcaca ttgcgaaaac cgaggatcaa accgccgcta atcaacgcgg
    16861 gcagcaacgg gaagaaggtc tctgcgaaat gagagatcaa ctgctcatgc catttcatat
    16921 tctgccgggc ggcttttttt acctgctctt tatcaacctg cgcctgtccg gttgacgcta
    16981 tcagtgcttg atagtagtca cccacgttgg tgccaatcac cacctgaaat tgcctggcat
    17041 tggtgaaaca gcctttcacc atggggagtt gctcaatttc tttcggtctg gcattggccg
    17101 gttggttgag gacaaagcgt aggcgagtaa tacagtggct caccgtcgca atattgccgc
    17161 gcccgccgac cagttcaatc aaccgatcga tatccgtttg gtttattttg ctcatcataa
    17221 agccccatgg cagatgacat ttttggttgg ctgcagaata tattgcgcgg aaaaatttaa
    17281 aaacgggaac gttcccgaaa cgcagcgaag atcacaattt atcgttcagg aaacgatctg
    17341 gacagggtgg cggggatgat gatttgttgc ggttcgctgc gcccggttac ctgtgcgatc
    17401 aactggcagg ccgcctggcg tccagcttcg gcgtaaccgg gatctacggt gacgatctcc
    17461 ggatggagga atttcattaa cggcgtatta ccgacgctcg ccagttgcaa ggtgtcgatg
    17521 cgttgctctt gcaggtattt acttgcgcca agtgccagcg tgtcggttgc gcacagtaag
    17581 gcggtagttt caggcgtaat cacttttgct acgttctcat agccttgctt catagcaagg
    17641 cctggcagag cggcaacggg atgcagttta tgcgctttgc agaacgccag gtaggcttcg
    17701 tgacgtcgct taccggtcgt cacgtcactg tgcggcacgc cgagataact gatattacga
    17761 tgcccctggt catacagccg ttgcatcagg atcttgattg ccccttcgtc gtcataacag
    17821 accgaagcaa agccttttgc gtcacgcgcc agcagaacca gcgatgactg ccagtgggct
    17881 aacatttctt ctgttatgcc ggtaaaaccg aacagcacta cgccgtcgat attacgccgt
    17941 ttcagcaccc ccaaatgttc ggcaactaat tgcggggaaa actgactttc catcatgatt
    18001 gggtcgtaac cttgttcata gaactctggc agcatggttt gaacggcgag attttctgac
    18061 aacgaatcca ggcgggtaac aatgatggcg accactttat cgctttgccc acgcatagcg
    18121 cgcgcagagc gggaagggga aaatccatgc tgattcatca ctgcttcaac acgctcgcgg
    18181 gtgcgctggc tcacgccgct ttcgttattc agcacccggg aaactgtaga tttccccacg
    18241 ccgcttaagc gcgcgatgtc tttgatggtc agccgatttt gcatcctgtt gtcctgtaac
    18301 gtgttgttta attatttgag cctaacttta cccgtgcatt cagcaatggg taaagtctgg
    18361 tttatcgttg gtttagttgc cagcaggtat tatatcgcca tagatgctac gaatattatt
    18421 ggattctcct tattatttgc ggcgcttttt tcacttaccg gaggttatat ggaacctgat
    18481 cccacgcctc tccctcgacg gagattaaaa cttttccggt aagcccgtct tttcacggcg
    18541 ttaccggatg cgtaaggccg tgacgtttta acgtccctgc tcagctttat taccttcagg
    18601 taaggcttcg ccacgcctga agacatttct gtactgtttc agacagtgcg gagggactcc
    18661 ttatgtttaa agaaattttt acccggctca ttcgccattt accttcccgt ctggttcatc
    18721 gtgatccatt gcctggcgcg cagcagacag tgaatacggt ggtcccgccg tccttaagtg
    18781 cgcattgcct gaaaatggcg gtgatgcccg aagaagaatt gtggaaaacg ttcgacaccc
    18841 atccggaagg gttaaatcag gcggaagtgg aatctgcccg cgaacaacat ggtgaaaata
    18901 aattacccgc acaacaactg tcgccgtggt gggtacattt atgggtctgc tatcgcaacc
    18961 cctttaatat tttactcacc attctcggcg ctatttctta cgccacggaa gatttatttg
    19021 ctgcgggcgt tatcgcgcta atggtcgcta tttctacgtt gctgaacttt attcaggaag
    19081 cacgttccac taaagcggca gatgccctga aagcgatggt cagcaatacc gcgacggtgc
    19141 tgcgcgtaat taacgacaaa ggcgaaaatg gctggctgga gatcccgatc gaccagctgg
    19201 tgcccggcga tattataaaa ctggcggcgg gggatatgat cccggcagat ttacgtatct
    19261 tgcaagcgcg ggatctgttc gtcgctcagg cgtcgttaac cggtgagtct ctgcccgtgg
    19321 aaaaagccgc taccactcgc cagccggagc acagcaatcc gctggagtgc gacacgctgt
    19381 gttttatggg caccaccgtg gtgagcggca cggcacaagc aatggtgatt gctacaggtg
    19441 ccaatacctg gtttggtcaa ctggcggggc gtgttagtga gcaggaaagc gagccgaatg
    19501 cctttcagca agggatcagc cgcgtcagta tgctgctgat tcgctttatg ctggtgatgg
    19561 caccggtggt gctgttaatc aatggctaca ccaaaggcga ctggtgggaa gcggcgctgt
    19621 ttgcgctttc ggtcgcggta ggcctaacgc cggaaatgtt gccgatgatt gttacctcga
    19681 cgctggcgcg cggcgcagta aagctgtcga aacagaaagt gatcgtcaaa catctggatg
    19741 ctattcagaa ctttggcgca atggatattc tgtgcactga taaaaccggc accctgacgc
    19801 aggataaaat tgtgctggag aatcataccg atatctccgg taaaaccagc gaacgcgtgc
    19861 tgcatagcgc gtggttgaac agtcattatc agaccggact taaaaacctg ctcgatacag
    19921 cggtgctcga aggtacggat gaagagtcag cgcgctcgct ggccagtcgt tggcaaaaaa
    19981 tcgatgagat tccgtttgat ttcgagcgtc gccggatgtc ggtggtagtg gcagaaaata
    20041 ccgagcacca tcagctggtt tgcaaaggtg cattgcagga aatcctcaat gtgtgttcgc
    20101 aggtgcgtca caatggcgag attgtgccgc tcgatgacat catgctgcgt aagattaagc
    20161 gggttactga tacgctgaat cgtcaggggc tgcgcgtggt tgcggtggcg acgaaatacc
    20221 tgccagcgcg tgaaggagat taccagcggg cggatgaatc cgacctgatc ctcgaaggat
    20281 atattgcttt tcttgatccg ccaaaagaga caaccgctcc ggcactgaag gcattaaaag
    20341 cgagtggaat taccgtaaaa atccttactg gcgacagtga gttagttgca gcgaaagtgt
    20401 gccatgaagt ggggctggac gcgggagagg tggtcattgg tagtgatatt gaaacgctat
    20461 ctgacgacga actggcaaat cttgcacagc gcaccacgct gtttgcccgc ctgacgccga
    20521 tgcataaaga acgcattgtg accttactga agcgcgaagg gcatgtggtt ggctttatgg
    20581 gcgatggtat taatgatgcg cccgccttac gcgcggcgga tatcggcatt tctgtggacg
    20641 gcgcggtaga tattgcccgt gaagcggctg atatcatcct gctggaaaaa agcctgatgg
    20701 tgctggaaga gggggttatt gagggacgtc gcactttcgc caacatgctg aaatacatca
    20761 aaatgacggc gagttctaac tttggtaatg tgttcagcgt gctggtggcg agtgctttct
    20821 tgcccttcct gccgatgttg ccgttacact tgcttattca gaacctgctg tacgatgtgt
    20881 cacaggtggc gatcccgttt gataacgtcg acgacgagca aattcaaaag ccgcagcgtt
    20941 ggaatccggc ggatctgggg cgctttatga tcttcttcgg accgatcagc tcgatcttcg
    21001 atattctgac gttttgcctg atgtggtggg tattccatgc caacacgccg gaaacgcaaa
    21061 cgctgttcca gtcgggatgg tttgtggtgg gcttactgtc gcaaacgctg attgtgcata
    21121 tgatccgcac ccgccgtgtg ccgtttattc agagctgtgc atcgtggccg ttaatgatca
    21181 tgaccgtgat cgtgatgatt gtcgggatcg cattgccgtt ttcaccgctg gccagttatc
    21241 tgcaattaca ggcgttgccg ttaagctatt tcccgtggct ggttgcgatt ctggcagggt
    21301 atatgacgtt aacccagttg gtgaaaggat tttatagccg tcgttacggc tggcagtaaa
    21361 gaataaacta ggcatggtgg cccggattca gattgcagac aaagtgagtt ttgttcatgc
    21421 cggatgcggt gtaaacgcct aatccggcct acaaattcaa tcaattgcag gaatcacgta
    21481 ggcctgataa gcgtagcgca tcaggcgatt ttgcgtttgt catcagactt aatccgggca
    21541 tgatagcccg gatttccatc aagattagcg acgaatagcg atcgcttcga tctcaatctt
    21601 tacgtctttc ggcagacggg caacttcaac gcaagaacgt gccgggaagg tggcgttgtg
    21661 ttcggtgaag aaggcttcgt aagtggcgtt tacggttgcg aagtcgttca gatcttttac
    21721 aaacacggta gttttaacga tgtcgcccac tttcagacca gcggcttcga cgatcgcttt
    21781 tacgttatcc agcgactgac gtgcctgtgc agcgacgtct gccggtactt cgcccgtttt
    21841 cggatttacc gggatctgac cggaggtgat gatcatattt cccagatcaa cgccctgtac
    21901 gtaaggaccg atagctgctg gtgcattttc cgtcgcgata gttttgctca tgatttctcc
    21961 tttattacag cggtaaaaag tctggctcat tatagggagc cagactttta ttaccaaccg
    22021 caattaattg gccagcacca cattatggga aaactctttt tcacagtatt tgcatttgag
    22081 cgcgatgtca ttggcgcgtt ttcgcacggc aaagctggat gaaaccggtt cggcatggct
    22141 gatacagttg ctgttcgggc agaccaacac attgtcgatg cgctctggca gactggggcg
    22201 cgatttaccc accacttcat agttgtcgat acggttaacc gtggcttgcg gcgcatacaa
    22261 tgccagttga tctacctgat cttcactcaa aaaggtattt tcgattttga tcagatcttt
    22321 gcggcccatc tcgccagaag gcaggttcag accaatggtg atgcgctgat ccgtttcggt
    22381 cagcttgaac agactcaaca gcttaaaacc gatctgggcg gggatatggt caattaccgt
    22441 gccgcgttta atagcttcaa cctgcaattt attatcgtgt gtcatctcta tttcccctta
    22501 cagtaccaga tcgcgattca gaaccagtgc cagtaacgcc tggcgagcga aaatcccgtt
    22561 gcctgcctgc tggaagtacc aggcgtgtgg cgttttatca acatccgtcg caatttcatc
    22621 aacacgcggc agcggatgca gtactttcat attggctttg gcgttgtgga gatcgctggc
    22681 gcgaagaaca aactgcgctt tcacgttggc gtattcggat gggtccaggc gctctttttg
    22741 cacgcgggtc atatacagga tgtctacttc cgccatcact tcttcaatag agctgtgcag
    22801 actccatgcg atcccttttt catcgagcat atccagaatg tattgcggca ttgccagcgc
    22861 gtccggcgcg atgaagtaaa aacggttgcc gtcgaacttc gctaacgcct gagtcaggga
    22921 gtgaacggtg cggccatatt tcaggtcacc aaccattgcg acgtggagat tgtccagacg
    22981 cccctgggtt tcctgaatag tgaataagtc cagcaaggtt tgcgtcggat gttggttgga
    23041 gccatcaccg gcattcagta ccggtacatt gccggaaaac tcggtggcca ggcgcgccgc
    23101 accttcctgc ggatgacgca tcactatcgc atcgacgtaa gtgctgataa ccgaaatggt
    23161 atcggccagc gtttcgccct ttttacccag tgatgtattg gcgctgtcgg agaagcccac
    23221 cacgctggca cccaggcggt gcatggaagt ttcgaaagag aggcgggtac gggtagaggc
    23281 ttcgaagaag cagctggcaa tgactttgtg cttcaacagc tctgattgcg ggtttgcttt
    23341 cagttttgcc gctgttgcca gcaccagatt aaggtcatcg cgactaaggt cgtttatgga
    23401 aatgatatgt ttctgatata gcggattagc catcttttat ctcctgacgc ctgggcaaaa
    23461 aaaagcccct cgattgaggg gctgggaatg ggtgatcaac gggaagaaaa acggcaggcc
    23521 agcgtctttt ttcagacgcg gtaagacaaa atgtcgaaca cactgaacca tacatcctcc
    23581 cggcaaattg tccggcatta tactcatcgt cagaagcggt gcaagcaatt gatgcaatat
    23641 tttgtcagcg caaacggttt atttgaattt aaagttaaaa tatatgcatt tttatttact
    23701 gtgattctgg ggggaactgt cgttcggtat caataattgc agacatttcc tgcttttttc
    23761 tttttcactt tcacgcaatc agatatgcat tttattccat tcatccgact tatagggcgg
    23821 agtgtgaaaa cgaacggcta gcactattgc ttagagctca gggatgcgcg ctatcacttt
    23881 aatttcaaaa tcaaagcctg ccagccatgt aacgcccacc gccgtccagg ttggataaga
    23941 tggcgcgcta aatatttcat ttttcaccgt catgatgtct tcaaattgtt tttctggatc
    24001 ggtatgaaag ctcgtaacat caatgatatc gtcaaaagtg catcccgcag ctgccagggt
    24061 cgcatgcaaa ttatcaaagg ccagtctgac ttgttgctga aaatcgggtt ctggtgttcc
    24121 gtcctctcga cttcctactt gcccggaaac aaacagcaaa tcgccggaac gaatagccgc
    24181 agaataacga tgctcagcat acagtgaatg tcgtccagca gggaaaacag cggttctttc
    24241 taccatttgg ttatcctcaa gatttacgac atgaacagaa gatttatatt taccaggagc
    24301 cgcttttagc ggacgacgtg agtaaacaaa acccagacat catgggaaat ggctgggctt
    24361 aattgagcgt agtcggttat gcgccaaacg cgccatcaat ggtatgcatc gcgccggtaa
    24421 caaaactggc ttctggccct gctaaccatg cgaccatacc agcgacctct tccggttgcc
    24481 catgtctttt gatggccata aaactatgcg acatatcgcg cattggcccg ttggcgggat
    24541 tagcgtcggt atcaattggc cctggctgga cgacgttgat ggtgatccca cgcggtccga
    24601 aatcacgggc cagcccgcgc gccatgcctt gcagggcaga tttactggcg gcataagcag
    24661 ccatgcctgc aacaggcata cgatcgccat tcacggagcc gatgattaag atgcgtccgc
    24721 cttcgggcat ctgccgggcg gcttcaacag aggcatggta aggagcatga atattgattt
    24781 tgaaaaggcg atcaatatcg tcggcattta attccagggc atcgccaaag acgccaatac
    24841 ctgcatttac caccaggata tccaatgcgc cgctcttacg aacgacatca ataacagcgt
    24901 ctttgtcagc actatctgtg aatactgctg tcgctccagt ctcttgtgcc aggcgttcag
    24961 cggcatcttt cgaccccgca taggtgaatc gtacattggc cccatcggtg acgaaacgac
    25021 gtacgatagc ggcaccgatt ccacggctac caccgaggat gagaactgtc ttacctgtaa
    25081 aagcgcccat aaggactcct tgatttatta tgtaacatgc attacaaaac tgttttaact
    25141 ttctgtcaac aagttttgta atggtcacta aaaaacaatc tcgcgttcca ggtcgtccca
    25201 gacgtttcgc tcctgagcag gcagtctctg cggcaaaagt gctttttcac caaaaaggtt
    25261 tcgatgctgt cagtgttgct gaagttactg attatcttgg tattaacccc ccgagcctct
    25321 acgcggcttt tggcaataag gctgggttat ttagccgtgt actcaatgaa tatgtcggta
    25381 cggaagctat tccgcttgcc gatattcttc gtgatgatcg tccggtaggg gagtgcctgg
    25441 ctgaggtatt aaaagaagcg gcgcgccgat atagccaaaa cggcggctgc gctggctgta
    25501 tggttcttga aggcattcat agttatgatc cacaagcgcg tgatattgcc gttcaatatt
    25561 atcacgccgc agaaacgacc atttatgact atatcgccag gcggcatcca caaagcgcac
    25621 aatgtgtgac tgattttatg agtaccgtga tgtcagggct ttctgcgaag gcacgggagg
    25681 ggcactccat cgaacaactc tgtgcaacag ctgcactggc gggggaagcg ataaaaacta
    25741 ttctcaagga gtgatgcggg ccttgacccg aaaggcggga acgcgcctgc cgataagttg
    25801 tgataagaca ataattcacg cgttaaggct agcggaattg atcatctttt cgtataacga
    25861 tggaaatgaa acgttgtttt aattaaggag tggaaaagat gatcatcgga aatattcata
    25921 atcttcagcc gtggctaccc caggagttac gccaggcgat tgagcatatc aaagcacacg
    25981 ttacggcaga aacgccaaag ggcaagcacg atatcgaagg caatcgtctg ttttatctta
    26041 tctcggaaga tatgaccgag ccgcacgaag ctcgccgtgc ggagtaccat gcccgctatc
    26101 tcgacattca gattgtgtta aaaggtcagg aaggcatgac cttcagcacg caacctgcag
    26161 gcaccccgga taccgactgg ttagctgata aagacatcgc atttttgccg gaaggcgttg
    26221 atgagaaaac agttatcctt aatgaaggtg attttgttgt gttttatccg ggggaagtgc
    26281 ataaaccgct gtgcgcagtg ggcgcgccag cccgggttcg caaagcagta gtgaagatgc
    26341 tgatggcgtg atgacttttc gccgtaaata actccaggtt tacggcgagt ttgtgaaaag
    26401 agcgtttttt gatatttttt tgtgagtaaa atttgtaatg cttagacgtt cttattaact
    26461 caaggagttc gtcatgagca aaatatcaga tttgaattat tctcaacaca ttacattagc
    26521 cgacaatttt aaacaaaaaa gtgaagtttt aaatacctgg cgtgttggaa tgaataattt
    26581 tgcccgtaat gccgaggggc aggataacac aagaaatatc cttgatccta agacattttt
    26641 ggaatttttg gtaaaaatat ttactctagg ttatgtggat tttagcaaac gctccaacga
    26701 agcgggaaga aatatgatgg ctcatattga gtcctcatct tatatcaaaa ataatgatgg
    26761 cagtgagata ataaagtttg ttatgaataa tcctgaaggg gaacgagcgg atttatctaa
    26821 ggtggaaata gaaatcacac tttccacttt cactactatg gggactcgtc aaggacatac
    26881 ggctattata tttcaacaac ctgatggttc gactaatcgt tatgaaggaa agtcctttga
    26941 aagaaaagat gagagttcat tacacctgat tactaacaag attctggcat gttaccaacg
    27001 cgaagctaac aaggaaatag ctcgtctatt aaatatttca caggagttaa ataattctca
    27061 ggacttgaat aactcgcagg tgagttgtaa agattcagtt gattctacga ttacggattt
    27121 attagaaaaa ccattgaata atgcattatt agcaataagg aaagaacatc tgctattaat
    27181 gccttatgta tgtaatgaat cgatttcata cttgcttggt gaaaaaggga tactgaaaga
    27241 aatagatgac cttaacgcag taaataatta tcttcttaat aataagaaag ctactgacaa
    27301 tgaaattaat gacatcaaag ttaacctgtc tcatattctt attgattcct tagatgatgc
    27361 aaaagttaac cttacaccgg tcatcgattc tggatgactt ttttaaaatc cccatatatt
    27421 aatgatgtaa gaatactgga ttggtgtttt aataaaagaa tgcaatattt tggtgattct
    27481 gaaaagataa agtatgcatg ctccgtgata aatcatattg attttagccg cgatcaatct
    27541 aaagatttta gctgcgatca atctaaaatt aaaatagctg agaccttatt tttcaatctc
    27601 gataaagaac cctataaaaa t
//
LOCUS       DAJVUJ010000040        27463 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.040, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000040 DAJVUJ010000000
VERSION     DAJVUJ010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27463
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.040"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            454..3639
                     /gene="rne"
                     /locus_tag="OQQ48_001563"
     CDS             454..3639
                     /gene="rne"
                     /locus_tag="OQQ48_001563"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HCS1952375.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAAPATPAAPA
                     QPGLLSRFFGALKALFSGSEETKPTEQPAPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPHHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(3836..4789)
                     /gene="flgL"
                     /locus_tag="OQQ48_001564"
     CDS             complement(3836..4789)
                     /gene="flgL"
                     /locus_tag="OQQ48_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HCS1952376.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFAIQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEEKGKYVGGAESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKEIAAAALDKTNRGLKNSLNNVLAVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(4801..6444)
                     /gene="flgK"
                     /locus_tag="OQQ48_001565"
     CDS             complement(4801..6444)
                     /gene="flgK"
                     /locus_tag="OQQ48_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HCS1952377.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNTQHKAGFDANGDAGEDFFAIGKPA
                     VLQNTKNKGDVAIGATVTDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEKDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSATQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(6510..7451)
                     /gene="flgJ"
                     /locus_tag="OQQ48_001566"
                     /pseudo
     CDS             complement(6510..7451)
                     /gene="flgJ"
                     /locus_tag="OQQ48_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
     gene            complement(7451..8548)
                     /gene="flgI"
                     /locus_tag="OQQ48_001567"
     CDS             complement(7451..8548)
                     /gene="flgI"
                     /locus_tag="OQQ48_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HCS1952378.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSARLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(8560..9258)
                     /gene="flgH"
                     /locus_tag="OQQ48_001568"
     CDS             complement(8560..9258)
                     /gene="flgH"
                     /locus_tag="OQQ48_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HCS1952379.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVVDARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(9311..10093)
                     /gene="flgG"
                     /locus_tag="OQQ48_001569"
     CDS             complement(9311..10093)
                     /gene="flgG"
                     /locus_tag="OQQ48_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HCS1952380.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMQQKLTQL"
     gene            complement(10231..10986)
                     /gene="flgF"
                     /locus_tag="OQQ48_001570"
     CDS             complement(10231..10986)
                     /gene="flgF"
                     /locus_tag="OQQ48_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HCS1952381.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKIDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHQVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAETQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(11006..12214)
                     /gene="flgE"
                     /locus_tag="OQQ48_001571"
     CDS             complement(11006..12214)
                     /gene="flgE"
                     /locus_tag="OQQ48_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HCS1952382.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLEFNANGTLVDGAMANNIATGAINGAEPATFSLSFLNSMQQNTGANNIV
                     ATPQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWS
                     ATQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQD
                     QILNTLVNLR"
     gene            complement(12239..12934)
                     /gene="flgD"
                     /locus_tag="OQQ48_001572"
     CDS             complement(12239..12934)
                     /gene="flgD"
                     /locus_tag="OQQ48_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HCS1952383.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTMLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAITASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(12946..13350)
                     /gene="flgC"
                     /locus_tag="OQQ48_001573"
     CDS             complement(12946..13350)
                     /gene="flgC"
                     /locus_tag="OQQ48_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HCS1952384.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(13354..13683)
                     /gene="flgB"
                     /locus_tag="OQQ48_001574"
     CDS             complement(13354..13683)
                     /gene="flgB"
                     /locus_tag="OQQ48_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HCS1952385.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTLGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYQMSLSALSGQIKGMM
                     NVLQSGN"
     gene            13839..14498
                     /gene="flgA"
                     /locus_tag="OQQ48_001575"
     CDS             13839..14498
                     /gene="flgA"
                     /locus_tag="OQQ48_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HCS1952386.1"
                     /translation="MLAIKRSVAIIAILFSPLSAASNLTSQLHTFFSAQLVGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAISLRDLSPDQPIQLTHFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARMRMVSGLVVSGVVDADGNILIN
                     L"
     gene            14574..14903
                     /gene="flgM"
                     /locus_tag="OQQ48_001576"
     CDS             14574..14903
                     /gene="flgM"
                     /locus_tag="OQQ48_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HCS1952387.1"
                     /translation="MSIDRTSPLNPVSTVQPRETTDAPVTNTRAAKTTDSTSTSTSTS
                     TSTSTSTSVTLSDAQAKLMQPGSSDINLERVEALKLAIRNGELKMDIGKIADALINEA
                     QQDLQSN"
     gene            14908..15324
                     /gene="flgN"
                     /locus_tag="OQQ48_001577"
     CDS             14908..15324
                     /gene="flgN"
                     /locus_tag="OQQ48_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HCS1952388.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGGKKISI"
     gene            complement(15364..16899)
                     /gene="murJ"
                     /locus_tag="OQQ48_001578"
     CDS             complement(15364..16899)
                     /gene="murJ"
                     /locus_tag="OQQ48_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HCS1952389.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(17066..17932)
                     /locus_tag="OQQ48_001579"
     CDS             complement(17066..17932)
                     /locus_tag="OQQ48_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCS1952390.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWMLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGE"
     gene            complement(17934..18581)
                     /locus_tag="OQQ48_001580"
     CDS             complement(17934..18581)
                     /locus_tag="OQQ48_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HCS1952391.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAELESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(18592..19176)
                     /gene="rimJ"
                     /locus_tag="OQQ48_001581"
     CDS             complement(18592..19176)
                     /gene="rimJ"
                     /locus_tag="OQQ48_001581"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HCS1952392.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            19412..20614
                     /gene="mdtH"
                     /locus_tag="OQQ48_001582"
     CDS             19412..20614
                     /gene="mdtH"
                     /locus_tag="OQQ48_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HCS1952393.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHRLM
                     AGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSYMG
                     FSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKRAA
                     RRLLERDA"
     gene            20678..21325
                     /gene="grxB"
                     /locus_tag="OQQ48_001583"
     CDS             20678..21325
                     /gene="grxB"
                     /locus_tag="OQQ48_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HCS1952394.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            21459..22019
                     /locus_tag="OQQ48_001584"
     CDS             21459..22019
                     /locus_tag="OQQ48_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCS1952395.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            22125..23171
                     /gene="pyrC"
                     /locus_tag="OQQ48_001585"
     CDS             22125..23171
                     /gene="pyrC"
                     /locus_tag="OQQ48_001585"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCS1952396.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVMPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHNFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREAHFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            23245..23490
                     /gene="dinI"
                     /locus_tag="OQQ48_001586"
     CDS             23245..23490
                     /gene="dinI"
                     /locus_tag="OQQ48_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HCS1952397.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGVTKEDKQRISEILQETWESADDWFVSE"
     gene            23780..24034
                     /gene="bssS"
                     /locus_tag="OQQ48_001587"
     CDS             23780..24034
                     /gene="bssS"
                     /locus_tag="OQQ48_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HCS1952398.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            24149..25267
                     /gene="solA"
                     /locus_tag="OQQ48_001588"
     CDS             24149..25267
                     /gene="solA"
                     /locus_tag="OQQ48_001588"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HCS1952399.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            25315..25428
                     /locus_tag="OQQ48_001589"
     CDS             25315..25428
                     /locus_tag="OQQ48_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HCS1952400.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            25689..26255
                     /locus_tag="OQQ48_001590"
     CDS             25689..26255
                     /locus_tag="OQQ48_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HCS1952401.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFTLGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            26259..26834
                     /locus_tag="OQQ48_001591"
     CDS             26259..26834
                     /locus_tag="OQQ48_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HCS1952402.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFEDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(26876..>27463)
                     /locus_tag="OQQ48_001592"
     CDS             complement(26876..>27463)
                     /locus_tag="OQQ48_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCS1952403.1"
                     /translation="RNHYEYEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMY
                     CTGGIRCEKASAWMKHNGFNKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGE
                     RISDEIIAHCHQCGAPCDSHTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPP
                     EEQRRRRAGRENGNKIFNKSRGRLNTTLGIPDPTE"
BASE COUNT         6588 a         7313 c         6919 g         6643 t
ORIGIN      
        1 gggatgcgtt catataaaaa ctgtgagtaa gcgggtgata aatggtaaaa gtcatcttgc
       61 tataacaagg cttgcagtgg aataatgaag ccgtttccgt gtccatcctt gttaaaacaa
      121 gaaattttac ggaataaccc attttgcccg accgatcatc cacgcagcaa tggcgtaaga
      181 cgtattgatc tttcaggcag ttagcgggct gcgggttgca gtccttaccg gtagatggaa
      241 atatttctgg agagtaatac ccagtctgtt tctttgataa ttgcgctgtt tttccgcatg
      301 aaaaacgggc aaccgacact ctgcgcctct ttgagctgac gataaccgtg aggttggcga
      361 cgcgactaga cacgaggcca tcggttcaca cccggaaagg cgttactttg cccgcagctt
      421 agtcgtcaat gtaagaataa tgagtaagtt acgatgaaaa gaatgttaat caacgcaact
      481 cagcaggaag agttgcgcgt tgcccttgta gatgggcagc gtctgtatga cctggatatc
      541 gaaagtccag ggcacgagca gaaaaaggca aacatctaca aaggtaaaat cacccgcatt
      601 gaaccgagtc tggaagctgc ttttgttgat tacggcgctg aacgtcacgg tttcctccca
      661 ctaaaagaaa ttgcccgcga atatttccct gctaactaca gtgctcatgg tcgtcccaac
      721 attaaagatg tgttgcgtga aggtcaggaa gtcattgttc agatcgataa agaagagcgc
      781 ggcaacaaag gcgcggcatt aaccaccttt atcagtctgg cgggtagcta tctggttctg
      841 atgccgaaca acccgcgcgc gggtggcatt tctcgccgta tcgaaggcga cgaccgtacc
      901 gaattaaaag aagcactggc aagccttgaa ctgccggaag gcatggggct tatcgtgcgc
      961 accgctggcg tcggcaaatc tgctgaggcg ctgcaatggg atttaagctt ccgtctgaaa
     1021 cactgggaag ccatcaaaaa agccgctgaa agccgcccgg ccccgttcct gattcatcag
     1081 gagagcaacg taatcgttcg cgctttccgc gattacttac gccaggacat cggcgaaatc
     1141 cttatcgata acccgaaagt gctcgaactg gcacgtcagc atatcgctgc attaggtcgc
     1201 ccggatttca gcagcaaaat caaactgtac accggcgaga tcccgctgtt cagccactac
     1261 cagatcgagt cgcagatcga gtccgccttc cagcgtgaag tccgtctgcc gtccggtggt
     1321 tcgattgtta tcgacagtac cgaagcgtta actgccatcg acatcaactc cgcacgcgcg
     1381 acccgcggcg gcgatatcga agaaaccgcg tttaacacca acctcgaagc tgccgatgag
     1441 attgctcgtc agctgcgcct gcgtgacctc ggcggcctga ttgttatcga cttcatcgac
     1501 atgacgcctg tacgccacca gcgtgcggta gaaaaccgtc tgcgtgaagc ggtgcgtcag
     1561 gaccgtgcgc gtattcaaat cagccatatt tctcgctttg gcctgctgga aatgtcccgt
     1621 cagcgcctga gcccatcact gggtgaatcc agtcatcacg tttgtccgcg ttgttctggt
     1681 actggcaccg tgcgtgacaa cgaatcgctg tcgctctcta ttctgcgtct gatcgaagaa
     1741 gaagcgctga aagagaacac ccaggaagtt cacgccattg ttcctgtacc aatcgcttct
     1801 tacctgctga atgaaaaacg ttctgcggta aatgccattg aaactcgtca ggacggtgtg
     1861 cgctgcgtga ttgtgccaaa cgatcagatg gaaaccccgc actaccacgt gctgcgcgtg
     1921 cgtaaagggg aagaaacccc aaccttaagc tacatgctgc cgaagctgca tgaagaagcg
     1981 atggcgctgc cgtctgaaga agagttcgct gaacgtaagc gtccggaaca acctgcgctg
     2041 gcaacctttg ccatgccgga tgtgccgcca gcgccaacgc cagctgaacc tgccgcgcct
     2101 gttgtagctc cagcaccgaa agctgcaccg gcaacaccag cagctcctgc acaacctggg
     2161 ctgttgagcc gcttcttcgg cgcactgaaa gcgctgttca gcggtagtga agaaaccaaa
     2221 ccgaccgagc aaccagcacc gaaagcagaa gcgaaaccgg aacgtcaaca ggatcgtcgc
     2281 aagcctcgtc agaacaaccg ccgtgaccgt aatgagcgcc gcgacacccg tagtgaacgt
     2341 actgaaggca gcgataatcg cgaagaaaac cgtcgtaatc gtcgccaggc acagcagcag
     2401 actgccgaga cgcgtgagag ccgtcagcaa gctgaggtaa cggaaaaagc acgtaccacc
     2461 gacgagcagc aagcgccgcg tcgtgaacgt agccgccgcc gtaacgatga taaacgtcag
     2521 gcgcaacaag aagcgaaggc gctgaatgtt gaagagcaat ctgttcagga aaccgaacag
     2581 gaagaacgtg tacgtccggt tcagccgcgt cgtaaacagc gtcagctcaa tcagaaagtg
     2641 cgttacgagc aaagcgtagc cgaagaagcg gtagtcgcac cggtggttga agaaactgcc
     2701 gctgccgaac caattgttca ggaagctcca gctccacgca cagaactggt gaaagtcccg
     2761 ctgccagtcg tagcgcaaac tgcaccagaa cagcaagaag agaacaatgc tgataaccgt
     2821 gacaacggtg gcatgccgcg tcgttctcgc cgctcgcctc gtcacctgcg cgtgagtggt
     2881 cagcgtcgtc gtcgctatcg tgacgagcgt tatccaaccc agtcgccaat gccgttgacc
     2941 gtagcgtgcg cgtctccgga actggcctct ggcaaagtct ggatccgcta tccaattgta
     3001 cgtccgcaag atgtacaagt tgaagagcag cgcgaacagg aagaagtaca agtgcagccg
     3061 atggtgactg aggtccctgt cgccgccgct gtcgaaccgg ttgttagcgc gccagttgtt
     3121 gaagaaatgg ctgaagtcgt agaagccccc gtaccggttg ccgaaccgca accggaagtg
     3181 gttgaaacga cgcatcctga agtgattgcc gccgcggtaa ctgaacagcc gcaggtgatt
     3241 accgagtctg atgttgccgt agcccaggaa gttgcagaac acgcagaacc ggtggttgaa
     3301 ccgcaggaag agacggcaga tattgaagaa gttgccgaaa ctgctgaggt tgtggttgca
     3361 gaacctgaag ttgttgctca acctgccgcg ccagtcgtcg ctgaagtcgc agcagaagtt
     3421 gaaacggtag ccgcggttga accagagatc accgttgagc ataaccacgc taccgcgcca
     3481 atgacgcgcg ctccggcacc ggaatatgtt ccggaggcac cgcatcacag tgactggcag
     3541 cgccctactt ttgccttcga aggtaaaggt gccgcaggtg gtcatacggc aacgcatcat
     3601 gcctctgccg ctcctgcgcg tccgcaacct gttgagtaat aattagctca aagtaatcaa
     3661 gccctggtaa ctgccagggc ttttttattt catgtttgaa tcaacatatt aaaattcatc
     3721 aaatggcatc cttgctaacc aacaatgcaa aataggcaaa aaaaatgccc gccccggcga
     3781 tccctgagcg ggcagacaaa acatacccag tttcatgata tgttttaaag cgagcttatt
     3841 tgttgagctg gaagagcgac aatccctgca tatcggtaaa tgctttatac gatgcctgca
     3901 atgccgtttg ctgcatgatg taagatgaaa tagttgcatt ccagtccaca tcaaccagat
     3961 cgctcatctg ctgcgtttgc cctaaagcgc gatcgctacc taaggaatcc agcgactcca
     4021 gttcgttcag ctgcgtgcct aattccgcgc gaacggcaag gacattattc agcgagtttt
     4081 tcagtccacg gttggtttta tccaatgccg cagcggcgat ttctttatcc gcttcgctat
     4141 ccgcgaccgg cgttttcagg gctgcgatgg cgctatccag catggcaaaa agattggttt
     4201 cagaagcgct accgtctggc tccgcaactg cgttgctggt aatactgtcg aaaattttgt
     4261 cacccgtgtg ccctatcacc atcgaacgcg aagcatcgac ctgttgctta atactttctg
     4321 ctccaccgac gtatttccct ttctcttcgc taaacggcgc agtctctgtt ttataaccgg
     4381 caaaaatgta gcgcccgtta ccgtcagtgg tgttcgccag attcagcaac tggtcacgaa
     4441 gcccctgaat atccgtagcc agcgaggccc ggtcatcgtc actcaaggtg ccattgctgg
     4501 cgtagacaat tttttcctga gcattctgga tagcagtggt gacctggcta agtacactct
     4561 cttccagtga cactttttga atggcgaaag tacgcgccag cgtgtactgg ctgttttgcg
     4621 cctgtgcctg ggagagaact acggcttgtg atgctgcaat gggatcgtca gaagggttaa
     4681 cgactcgctt acctgtcgac atctgctcgc cgtacttcat ccattccgcc tgagaattgg
     4741 tgatgccacg catgttttgc tggtacatca tctgggtact gaaacgcatc ttatctcccc
     4801 ttagcgaatg ttaatcagcg catcaaaaat cgcgtttgcc gtctgcagaa cctgcgcatt
     4861 cgccaggtaa tactgctgaa aacgttgcag atttccgtac tcttcgtcga gattgacgcc
     4921 agagatagac tgctgctgat tggaaagctg cgtcaccaca ttaccttgcg tggcgctact
     4981 ggttttcaac gtcgcggttt tattaccgat atcactcact aacgaggcat aagcgtcgtt
     5041 aaaggatttc gcaccgccca ccgttttact gttgctttgc agatccagca gggcctgacc
     5101 gttgcggtta tcgctatcac ccgcatcttt ttcgctcgcc atcgctattt tcgcttcgtc
     5161 ggtgattaat acatccatgt tgacgatggc gtcacttact ggtttcagcg tgaagctgtc
     5221 gttaacggca ggcgttcctg taaacgtcaa ctccagacca tcaaatgcca ctttaccgtt
     5281 ggcatccggc gtcaccgtaa aagtggtatt gctggcaagg cgggtgacct gccactgatt
     5341 attatcgaac gagattttgt aatctgtcgc cagtaccgcg gaggcatcag ttaccgtggc
     5401 accgatcgca acgtcacctt tgtttttcgt gttttgcaaa accgcgggct taccgatagc
     5461 aaagaaatct tcaccagcat cgccgttggc atcaaatccg gctttgtgtt gggtgttgaa
     5521 agcctcagca aatgccagcg ccagttgtcc aagcgtatta cgcgtctggt ccagatcctg
     5581 agaacggaat gtcagaatgc cgcccagcga cccggtattc agtaatttct ccgggatctc
     5641 aatattgcct gccgtcccat caacgtaagc gacagtcgta cgagaagggt cggcgctgga
     5701 aggaactgcc gccagttgcc gcgccgtact tccctgaacc agtgagtaac cattggccat
     5761 cgtgatgtta taagtgccgc catcctgaac gctgacttct acaccaacaa tctggtttaa
     5821 ttcgctcacc agttgatcgc gttgatccag cagattgtta ggtgacgccc ctgcccccac
     5881 gcctgtcagg cgggagattt ggtcgttcag gctggcgatt tgtttagcgt aattgttgat
     5941 ctgatcaacg ctggcaccta tcgcgatatt gacctgtttg tcctggtcgc gaagatactg
     6001 atcggtggtt ttaaactgat tcaccaaccc ttctgatttc ccaatcagcg cctggcgcgc
     6061 tgccgggtct tccgcgttac tcaccagcgt ttgcaggctg gtgaagaaat cctgcatctg
     6121 tgttgccagc gaagaggtac tggtggagag catattgtcg attttcgaca tctgctcata
     6181 gcgggcagtc aggccgctac tttgcgtctg cgccgcacgt aactggttgg tgataaacgc
     6241 atcatactca cgctgcacac cagaaacgta gacaccattg ccaacccagc cgccagcgcc
     6301 caacgtgcta ttggcctgcg ccataatagt ggtttggcgg gtatatccgg caacgttata
     6361 gctggagata ttattacttg ccgtatttaa cgccgcctgg gccgcgttca gtccgctcat
     6421 ggcgttatta atcaagctgg acatggaggt tccttataag ccttcaatta cagagtatta
     6481 tcggcagcga cccgccggac ttgagttatt caaaacagat tatcaatgtt catactgtag
     6541 gttttgctca ccttgtcgct tatcgatttc atctgctgaa tcatgttggt gagtttgcgg
     6601 gcatagtgag gatcggtggc atagcccgcg tcctgtaggg cctgcgcccc ctgctccgca
     6661 ctcgtggcgg tggtcacggc ggcgtagcgc gggttacgcg ttaacagccc aacgtaatcc
     6721 gactatgctt ccagatacga gctgtagacc cgaaacttcg cttttacttt cttcgcttcg
     6781 ccgttttcat attcagtcgt ggtgatttca gtaactggcc ctttccagtt gccagaggct
     6841 ttgacaccaa acaggttata gctcggctcg ccgttttcgc ggcggatttg ccgttgcccc
     6901 caaccagatt ccagcgccgc ctgagcgagg atcaaatgat gtggcacacc gctttgctgg
     6961 cttgccagtt gggcgggcag cgagagttgc gcgaggaatg ctttactgtc acccggcagc
     7021 gaatcatcgt agttacgtgg cacggccttt tgcaccagct gcgaaagcgc ctgattttga
     7081 taacgcacca cagtttcgag cgggaatttc atcggtgctg ctggcgtgga ctcctctggc
     7141 aatggttgtt ctggcgtcat ctgtttaacc atcatctctg caagccccag acctttgccc
     7201 gccgtcatct gttgggcaat ctgctggtca tacatactgg tatacaggcg agtgtgctcg
     7261 ctgctgaaca ggccatcttt tggtaaagcg tcgcgcatgc ttttcaacat catctgcacg
     7321 aacatccctt ccacctgacg ggccaccgga cggatatttg ccgccggatc ttcgcccgct
     7381 ttcgccttta gttcgttgag tgattgcgca tcccaggccg cacttgccag tagtttgcta
     7441 tcgctgatca tcagatgatt tccagttttg cccgcagaca tcccgcactt tgcattgatt
     7501 gcagtatgga catcagatcc atcggcgtag cgcccagcgc attgagcgcg cgcaccacgt
     7561 tattgagcct ggcgctggaa cgtacgcttt gcagcgaacc gccgctctgg cgtaaatcga
     7621 tctgcgtttg tggagtaacc acagtctgtc caccaccaaa cggtgtatct ggctggctga
     7681 cattggcctg acgattaact gttactgaga gattcccctg cgctaccgcg cagctgtcga
     7741 gggtcacttc gcgattcatc accaccgaac cggtgcgcga gttaatcact actttagcgt
     7801 cctgcggggt gacattaacc tgcatattct ggatatcggc aaggaagcgg acctgggaac
     7861 tgttgccact cggtacgcgc acctgaatag tccgcgcatc taacgcggtg gcgctgccat
     7921 atccacgcac gcggttgatg gtgtcagcga tttgctgcgc catgctgaaa tcttcgtcgt
     7981 taagttgcaa attaagggta ttcccgacgc caaactggct gggcaattca cgttcaataa
     8041 ccgcaccatt ggtgatccgt ccaccgttca gttggttaac ctgaacactg ctaccgccag
     8101 cggaggctcc tgcgccgcca accagaatat tgccctgcgc cagcgcatac acctgactgt
     8161 caacgccctt aagcggtgtc atcaacaacg tacctccacg caagcttttg gcattaccca
     8221 tggaagaaac caccacatcg atggtttgcc cctgacgtcc aaacggagga agtgacgctg
     8281 tcaccattac cgcagcgacg ttttttagct gcatattggt gcccgtcgga acggtaattc
     8341 ccagctgtga gagcatgtta ttaagcgttt gtgtggtaaa cggcgtctgg gttgtctggt
     8401 caccggtgcc atccagcccc accaccagac catagccaat cagtgagttt tgccttaccc
     8461 cctgaacact ggtgagatcg cgaatacgct cagcctgagc cgccgtcgtg accagtagaa
     8521 gaattaatgc agagagaaat ttaatcacca cagcctcact tacattggcg acaggttaag
     8581 gaagaaacgc tgcaaccagc ccatattttg cgcttcgtta atgtagccat tgcctacgta
     8641 ttcaatgcgc gcatccacca cctgagtaga cggtacggta ttgctgccgc tgatagtgcg
     8701 tggattaacc acgccagaga agcgaataaa ttcggtaccc tgattaatgg caatctgttt
     8761 ttcacccacc acatgcaggt tgccgttgac cagtacctgg tcaaccgtca ccgtcaacgt
     8821 gccgctaaag gtattgctgg cattggcccc gccctttccg ttgaacgtgt taccaccgga
     8881 ggcttcgaca tcggcacgag cgttaccaaa cagcccctgc aaatagcgcg gcacagtatc
     8941 aaagccaaaa ttagttttac cgtcacggct ggcattcgca gaggagcttt tgctggcgct
     9001 gacgttctcc tgcaacacga tggtcagcgt atcgccaata ttgcgtggtc gacgatcttc
     9061 aaacagcggt tgatagccat agttaatcgg ctgagcagac tggaaaatag aaccgttggc
     9121 gacgggcgtc ggaccgggaa ccggttgtgc actggtcgcc ccctgcacca gcggcgtgga
     9181 gggtatccag gcgcagccgg ttagtgaaag caccaacaag ctggaaatgg cataagtatg
     9241 cgcagcgttt ttttgcatgg ctatcatctt caaaaatcag taaaccggtg aacctgccac
     9301 cggttaagcc ttagagttgc gtcagttttt gctgcatctg atcggtggtg gacaccgctt
     9361 tactgttgat ttcgtaagcg cgttgcacct gaatcatatt gaccagttct tccgccacgt
     9421 tgacgttaga cgtttcaaca tacccttgat acagcaatcc cgcgccgttc aggcccggcg
     9481 tgctttcgtt cggtgcacca gaggattgcg tttcggtgta gaggttttcg ccaatgctct
     9541 ccagcccggt gtcattcata aaggtggtga gattgagctg cccaacctga accggagctg
     9601 cctggccttg ttgggttacg ctgaccacgc catcacgacc gatggtgata cttaacgcat
     9661 tcgccggaat ggtgatcgct ggctgcacct gaaaaccacc agccgtcacc agctgcccgt
     9721 tctgatccac ctggaaagag ccgtcacggg tataggctga tgaaccatct ggcaacatca
     9781 cctggaaaaa gccctgccct ttaatcgcga catctttgct gttgttggtc tgcgacagat
     9841 ttccctggct gtgtaagcgt tcagtggcga ccgggcgtac ccccgtgccg atttgtaatc
     9901 cggagggtaa ggtggtttgt tcggaagact gtgcccccgg ctggcgaatg gtttgataaa
     9961 gcagatcttc aaacaccgcg cgctgacgct taaaaccgtt agtactgacg tttgccaggt
    10021 tgttggcaat gacgtccata ttggtttgct gggcgtcaag gcccgttttg gcgatccata
    10081 atgaactgat cataaaatgt cctgtagtgg attagtgaca agcagagcag aagcacgcga
    10141 cagtgtgaat tccccctcac tctaaccctc tccccaaagg ggcgagggaa tagtccgcgc
    10201 gcttttattg caatgtcatc aatcgttcaa ttaactcatc gacagcagtt ggttggcacg
    10261 gcctgcgtta tcatcgacgc tgctgatcac cttcatctgc atttcaaaac gccgcgcgct
    10321 ggcaatcatg tcgctcattg ccgcaacggc attgacgtta ctgccttcca gaacccccga
    10381 catcacacgc aaggttggat ctgcctgcag taccggccct cgcgtggcct gggtttctgc
    10441 gcttaaacga aaaatgccgt cgtcaccgcg ctgcacttcg ctgcccgtgg ctttcaccag
    10501 tttcagacgc cctactggcg caactgtatt tgccggatcg cccggattga gcgccgagat
    10561 tgtgccatcg gcagcaatag tgatttccgc tccttccggc acagcaattg gccccgcctc
    10621 gcctatcacc tgatgcccct gaattgtcag ttgcccggtg ggatcaacct gaatgctgcc
    10681 attacgcgta tacccttcgc tgccgtcagc ggtctgcacg gccagccagc catcctgctg
    10741 caacgcaacg tccagcgggc gcgaggtgta atctatcttg ccgggcgtca tgtctgcacc
    10801 cggtgttgac gccgttacca gcgtgcgcgt gggcagtgaa agcccttcca ccggcactgc
    10861 gcgcaaggcg tttaactgcg cgcgaaaacc gggcgtagag gcattggcca gattactggc
    10921 ggttaccgcc tgttgattca gcgtctggct ggctgctccc atcgcagtat aaattgcgtg
    10981 atccattgag ctatcccgtc agcgattagc gtaagttaac cagcgtgttg aggatctggt
    11041 cctgggtttt gatggtctgg gcgttagact gatagttacg ctgggcaacg atcatattga
    11101 ccagttcttt actgagatcg acgttggacg cttccagcgc accgttggtc agggtgccaa
    11161 agtttcccgt cccggctgtc cccaacagcg ccacgccaga agattgcgtc gcagaccaga
    11221 cgttgtcgcc ttcggatgcc agaccttcgt tgttggcaaa gttcgccagt acaatctgcc
    11281 ccagcagttg agtttgttcg ttggaatagt tgccgacaac cgtaccgtca tcattgattt
    11341 gataactcac cagatcgccc ggtttgtagc cgttctgggg ggttgccaca atattgttag
    11401 cgccggtatt ttgctgcatg gagttgagga agctcagact aaacgtggcg ggttctgcac
    11461 cgttaattgc gccggttgcg atattattcg ccatcgcacc atccactaat gtgccattag
    11521 cattaaattc cagtgttgtc gctgtcttcg caatgctgtt tggatcactg ctatcctggg
    11581 tgtagacctg ccagttatta tccccggtct tcacaaagta gacgctcatg tcatgagcat
    11641 taccctgact gtcgaaaaca gtcaccgaac cttttttgtt atagctatcc gcattgctgg
    11701 cgctgaatgg cgtaacagta ggaagcggat cactggaatt caggttgatc tgcatcgacg
    11761 ccgtggtggt agttttcgct gccatcaggg tattcgggat cgaaatattg gtcggattcg
    11821 ccccttgctg aatagtcggc ggcgtaccgg ttgccgggta acccgtcagc tgtaaacctt
    11881 gcatattcac caggttacgg ttttcatcca gcttaaattg tccgttacgg ctgtagaaca
    11941 ccgaaccgtt gctgtctacc agacggaaaa aaccgttctg gctgatagca acgtccagac
    12001 ctcgcccggt gttggtggtc gtgccatctg taaagtcctg agtgataccg gcaactttta
    12061 cccccagtcc cactttcgaa ccggcaaaca tatcggcaaa agaggccgtg cctgatttaa
    12121 agccgtaggt ggcggagttg gcaatattgt tgccaataac atcgaggtta gtggcggcag
    12181 cgtttaatcc gctaaccgct tgagaaaagg ccatgactga ctcctgaaat gtgaaggctt
    12241 aaattatctg ccgtacttcg tcgagggtgg tggtgccgta agtgccgaga tccagcgtat
    12301 taccgctgtt gccgcggatc acaccctgca ccagagcaaa ctgcagcggc tgggcaacca
    12361 gttgtgtacc accgttactg gcggtaatcg ctacattgta agaaccgttc ggcgcagtgc
    12421 tgccatcagt caacgtaccg tcccaggtga aactgtgaac tccggcggtc agttcaccaa
    12481 tatcaatggt gcgcacaacc gcgccatttt tatcggtgat ggtggccgta actttgtctg
    12541 ccgcctgttg cagctcaaca ccaaacggcg tggtcgtggt cacagcccct tcttcactgc
    12601 cggttcccgc aagaacagtt gtgccgggga tcatcacgcc gtgaccgatc aggttactgg
    12661 cctgtaacga ctggctgtta tcaatctgtc cggaaataga tccgagcatg gtattgagtt
    12721 tttcaatccc gctgaccgtg ctgatttgtg ccaattgcga cgtcagctcg ttgttttcca
    12781 ttggattggt cgggtcctgg tttttcagct gcgccaccag caaagtcaga aaactgcttt
    12841 gtaaatctgc ggcgttgctg cccgtgagcg aactactgct ggtggtactg acgccggtat
    12901 ttgtcggatc ggtggtggtt accgcaatgg acatagcttt ctcctttatt gaccgagcgt
    12961 aagggttttc agcatcatgc ttttcaccgt gttgagcact tcaacattgg cctgatagct
    13021 gcgtgacgcc gacatggtgt taaccatctc tccgacaaca tcaacgttcg gcatttttac
    13081 gtagcccttt gcatccgcca gcggattacc cggttcataa accagtttgt ccggggcctg
    13141 actttctata acatcggcaa cctttacgcc gcctgtcgca gcacctggtg cagcgttaac
    13201 ctggaatacc acctgttttg cccgatatgg ctgtccatcg ggaccggtca cgctatcagc
    13261 attcgccaga ttactggccg ccacgttcag gcgctgggac tgggcagtta acgccgaccc
    13321 ggcgatatca aaaatattca gcagtgccat ccgttaattt ccgctctgta aaacgttcat
    13381 catgcctttg atttgcccgc tcaacgcact aaggctcatc tggtattgca gttctgcggt
    13441 aggaggcgtc agcgcctgcg ccggaatgtg ttgcgttgag gtcatcgtca gagcaaccac
    13501 actggttgca tcccgtccac gttgcatgac ttttttaagt tcactggcaa aatcgatatc
    13561 gcgcgcctga taaccaaggg tatcggcatt ggcgatgttt gctgccagca cttcctgacg
    13621 ctgggcgcgc agattgagcg cctcttgttg aaaacgtaag gcggcgtcga gcttatcgag
    13681 catatctcct ccgcaggtat caaaattctg ccatcagctt aaatgccttt acgcgcgcgt
    13741 tatcggcgga ataaacgcaa aatgggtcgc tatttatgcc gttgatgacc attggggacg
    13801 ggtacaattc acgttgtaga aatagctggg gggcgaaaat gctggcaata aaacgtagcg
    13861 tggcgatcat cgcgatactg ttcagtccgt taagtgcggc gagcaatctc acatcgcaat
    13921 tgcacacctt ttttagcgcc cagctcgtgg gggtaagtga tgaggttcgt gtttctattc
    13981 gtacagcacc caatctacta ccgccgtgcg agcagccatt gctttcgatg agcaataatt
    14041 cccgcctgtg gggcaatgtg aatgtgttgg cacgctgcgg taacgacaaa cgatatttac
    14101 aggttaatgt gcaggccaca ggaaattatg tggttgccgc gatgcccatt gtgcggggag
    14161 gaaagctgga agctggcaat gtcaaactaa aacgcggacg actggatacc ctgccaccgc
    14221 gtacggtgct ggatatcaat caacttgttg atgccattag cctacgcgat ctatcacccg
    14281 atcaaccaat ccaattaacc cattttcgcc aggcatggcg ggtaaaagcg ggacaacgcg
    14341 tcaacgtgat cgccagcggt gacgggttta gcgccaacgc agaaggtcag gcgctgaaca
    14401 atgcagccgt cgcacagaat gcgcggatgc gcatggtatc gggactggtg gtcagcggcg
    14461 ttgttgatgc agatgggaat attcttataa acctgtaagc tgttaaagat tacccgtccc
    14521 ttgccgataa ataagcaaca catgataaaa gcgccctcaa tgaggaataa accatgagta
    14581 ttgatcgcac ttcgcctctg aatcccgtaa gcaccgttca accgcgcgaa accactgacg
    14641 cgccggtaac gaacacccgg gcggcaaaaa caaccgactc caccagcacc agcaccagca
    14701 ccagcaccag caccagcacc agcaccagtg tgacgttaag cgacgcgcaa gcaaaactga
    14761 tgcaacccgg cagcagtgat atcaatcttg aacgtgtcga agcgttaaaa ctggcgattc
    14821 gtaacggtga actaaaaatg gacatcggca aaattgccga tgcgctgatc aacgaagctc
    14881 agcaagactt gcagagtaac tgatcgtatg acacgtcttg cagagatcct tgaccagatg
    14941 tccgctgtgc ttaacgatct caaaacggta atggatcaag agcagcaaca tctctctatg
    15001 gggcagatca acggcagcca gttgcaatgg attacagaac aaaaaagctc actgctggcg
    15061 acgctggatt acctcgaaca gttacgcaag aaagaaccca atacagcaaa tagcgttgat
    15121 attagtcaac gctggcagga aattactgtg aaaacgcagc aactacgcca aatgaatcaa
    15181 cataacggtt ggttactgga aggacagatt gagcgcaatc aacaggcgct ggaaatgttg
    15241 aaaccgcatc aggaaccgac gctatatggg gcgaacggtc agacctcaac aacccatcgc
    15301 ggcggtaaaa agatttcgat ctgaagatta tctccggcct gcactgcagg ccggaatgca
    15361 ttgttacacc gtccggcggg caaattcttt aactttgaag cccagtaccg ccagtgcagc
    15421 gaagtacgcg gcaatccccg ccagcacgac cgccattaaa cgcagtaagc gccagggcat
    15481 ggtacccaat gaccactccg gcatgatatg taacatacct aaaagcacgc cagacatcac
    15541 cagtaccgcc accaccagac gcaacagaaa cgccatccag ccgggttgcg gagtaaagat
    15601 tttctgctta cgcaactgcc agtaaagcag cgaagcattc agacacgccg ccagaccaat
    15661 agaaagtgac agcccggcat gtttcaacgg accaataaac gccaggttca tcaattgcgt
    15721 taaaatcagc gtaacgatgg caattttcac tggcgtttta atgtcctggc gggaataaaa
    15781 gccaggagcc aacactttca ctacaatcag gccaatcaaa cccaccgagt aggcaattaa
    15841 cgcccgctgg gtcatcagcg catcaaacgc ggtaaattta ccgtactgga acagcgaaac
    15901 ggttaacgga cctgaaagaa tgcccaacgc aaccgcactc ggcagcgcca acaggaaaca
    15961 aagacgcaac ccccagtcca tcaaacggtt gtattcatca tgattgccac tggcaaaact
    16021 tttcgacagc gacggcagca aaatggtgcc aagcgccacg cccagcacac cggacggaaa
    16081 ctccattaag cggtcggcgt aatacatcca agacaccgaa ccggaagcaa gaaacgaggc
    16141 aaaaatggtg ttgatgatta aggagatctg gctcacagag acgccaagga tcgccggtcc
    16201 catctgtttc accacgcgca ttgctccggc atcgtggaag ttaatgcgcg gcaggaccag
    16261 catgccgatc ttcttcaggt gcggtagctg atacaccagc tgcaggatgc cgccgaccgt
    16321 aacagcccac gccagcgcca gcaccggtgg gttaaagtac ggtgcggcaa acagcgcgaa
    16381 accaatcatg ctgatgttaa gcagtgttgg cgcaaacgcc ggaatcgaga agcggttcca
    16441 cgtattcaga atcgctccca ccagcgacgc cagggagatc agcaagatat agggaaaggt
    16501 aatctttagt agctggctgg tcagggcaaa tttgtcagct gtatcagcga agcctggcgc
    16561 ggtcaccatg atcacccacg gtgcggcgag catgccagcg accgtcacaa ccgccagcgc
    16621 aagtgtcagc aggccagaaa cataagagac aaagacccgc gtggcgtctt caccctgctt
    16681 acttttatat tccgccagaa tcggtacaaa tgcctgggaa aatgcccctt cggcaaagat
    16741 acggcgtaac aagttaggaa gtttaaaagc gacgaaaaag gcgtcggttg ccatccctgc
    16801 gccaaagatt ctggcgacaa ttgcgtctcg tgcgaagcca agcacacgcg aaaacatggt
    16861 catcgagctg acggcggcca gcgattttaa taaattcatc ggtgttctaa tccagaccaa
    16921 cgggcaaaac gcctgcaaga caggcgctga aagaggcgaa tagtctacct ggattatggt
    16981 gaattgctac cgccagatgt tacagggttt attcactcat cgcatcgcgc cagatcttgt
    17041 caacgatacg ttgcgccagc acggcctatt cgccggcggt ttgcggaact gtctgatttt
    17101 gcacacattc aatgaagtgc cgcgcacagc cgacaaatcc acgttgttca agagtgctct
    17161 gccaaccagg aatcggttta tgcactacgc cctgcccgcg ctcctcacgc cattcgcgca
    17221 tatccgtaat gtcgatgagc gcaccgtcag tcacggcctg cacggtttca cgctgacttc
    17281 cggcacggcg atgcatacag gtggtgatct gcaaaggacc ggccgaaaaa tggtgctcgg
    17341 caaacagcat ttcgccagcg tcgttagtca gtagcgtacc gccatccaga gaggctttgc
    17401 cgcccgacaa ccacagcgcg gtatccacca catgcagata atcatccagc aacgtgaaat
    17461 aaagatcgtg tggcccgaca ctattgctac gatgtttatc cattcttagc gaagctgcgg
    17521 tagcgagttg cgtttttaac tcaccgtaga gtggtgcgaa acgacggtta aaaccgacca
    17581 tcaacgtcag ttttttccgc gccgccagtt ccaccagccg ttcagcatcg cgcagatttt
    17641 ctgccagcgg tttatcgaca cagacatgta cacccgcatt gagtaacgta ctgaccacgt
    17701 caaagtggct ggcggtgctg gaatgcacaa aaaccgcatc gcaactggcg gcaaggctgg
    17761 ataacgaatc ggcataagga atgcgccagc tttcacaaat tggcagggct ttcgcgcgcg
    17821 taggcgacca ggctccttgt aacatccagt cagacgctgc cgccagcacc ggtaaccacg
    17881 ctttttgcgc aatgccgcct aatcccacaa cgccgatacg taattttttc actttaatct
    17941 cccagatggg ccagcaacga atcaagacgc tgtttcagtt ctgccacttc gatttccagg
    18001 gcttcgacgc gggcctgtaa atcaccatca accgcgttcg acatatccgt taccgccggc
    18061 tgatcctcta cctcaccact gaaaagatgc atgtagcggc tttcgcgttt acccggttcg
    18121 cgggccagac gcaccacaaa aggaccatct tcgcgatttg ccagttgttc cagcgtcgac
    18181 tccaattccg ccatatcgct gaattcatac attcgcgcgg cgcggctgcg cagttcaccc
    18241 ggcgtctggg caccacgcaa taacaacgtg gtgattaacg ccacttccgc tgcgctcagt
    18301 ttcagatcgc caaattctga attacaaaaa cgttgttcat atttggtgac ccgattacca
    18361 aagccactca ccgtgcgcag ataatggcgt ttgaccagat tatccagttg ctcctgcact
    18421 tcagattcac tcagattcat gaccggttca cggttcgttt tctgattaca ggccgtgact
    18481 acaccattga ctgagagcgg gtattgctcc ggcgtggtca cctgcttttc cagcaagcaa
    18541 ccaatcacgc gagcttcaag ggcagtaagt tgatatttca tgagattctc cttagcggcc
    18601 gggcgtccag tctggggtag ttaatgccgt cagtacgtga tcgcgccatt gtccatcaat
    18661 caacagatag tctttcgcat agccttcttt ttcaaaaccc agtcgcgcca gtaaatcacc
    18721 gctgcgttta ttgtgcggca tataattagc cataatgcga tgaatatgtt gggtgcgctg
    18781 catataacga atggctgcgg tcagggcttc aaacatgagt cctttgccct gccatttttg
    18841 cccaatcgaa taaccgagat agcaggcatg aaaagagcca cgaacaacat tggaaaaatt
    18901 ggcaacgcca ataatctctt tttcgtccgg gtcgaataag ccaaagtaga aagctgaacc
    18961 ttgtttatga aattcgttaa tcatccccag cctggcctgc cagcctgatg gataacagtg
    19021 gctttcgtcg cgcactggct cccagggctt gaggaaatgg cgattctctg cgtaataatc
    19081 cgcaagacgc caggcatcac gatcatgcac cagacgcacg accagtcggt ctgtggttaa
    19141 gcgcactttt ggcacgttac tgcgatagcc aaacattcta tacctactcc ttcccgtaac
    19201 gtctttaata cgctgtaagt attactatac ctgcgcaacc agaccctgtg aaaagtccaa
    19261 aatgccattt tctgcattat tcacatattt gataaaaact ctctatcaaa gcgacgtttt
    19321 gataaaaaaa tattgtcccc gaagtgatgg gaaaagtctt agcggcaatg cagaatagaa
    19381 agtgctcatc ttttatttcc cgggagggga aatgtcccgc gtatcgcagg cgaggaacct
    19441 gggtaaatat ttcctgctca tcgataatat gctggtcgtg ctggggttct ttgttgtctt
    19501 cccgctgatc tctatccgct tcgttgatca aatgggctgg gccgccgtca tggtcggtat
    19561 tgctctcggt ctacgccagt ttattcagca aggtctgggt attttcggcg gcgcaattgc
    19621 cgaccgcttt ggtgccaaac cgatgattgt taccggtatg ctgatgcgcg ccgccggatt
    19681 cgccacaatg ggtatcgccc acgaaccgtg gctattgtgg ttttcatgcc tgctctcggg
    19741 actcggcggc acgttgtttg atccgccgcg ttcggcgctg gtggtgaaac taatccgtcc
    19801 acaacaacgt ggtcgttttt tctcgctgtt gatgatgcag gacagtgccg gtgcggtcat
    19861 tggcgcattg ttggggagct ggctgttgca atacgacttt cgcctggtct gcgccacagg
    19921 ggccgttttg tttgtgctgt gtgcggcgtt caatgcgtgg ttgttaccgg catggaaact
    19981 ctccaccgta cgcacgcccg ttcgcgaagg catgacccgc gtgatgcgtg acaagcgttt
    20041 tgtcacctat gtcctgacgc tggcgggtta ctacatgctg gctgtacaag tgatgctgcc
    20101 aattatggtc aacgacgtgg ctggcgcgcc ctctgccgtt aaatggatgt atgccattga
    20161 agcgtgtctg tcgttaacgt tgctctaccc tatcgcccgc tggagtgaaa agcattttcg
    20221 tctggaacac cggttgatgg ctgggctgtt gataatgtca ttaagcatga tgccggtggg
    20281 catggtcagc ggcctgcaac aacttttcac cctgatttgt ctgttttata tcgggtcgat
    20341 cattgccgag cctgcgcgtg aaaccttaag tgcttcgctg gcggacgcaa gagctcgcgg
    20401 cagctatatg gggtttagcc gtctgggtct ggcgattggc ggcgctattg gttatatcgg
    20461 tggtggctgg ctgtttgacc tgggcaaatc ggcgcaccag ccagagcttc cgtggatgat
    20521 gctgggaatt attggcatct tcactttcct tgcgctgggt tggcagttta gtcagaaacg
    20581 cgccgcgcgt cgtttgctgg aacgcgacgc ctgatttgat actcccccct gactcctcca
    20641 tactgaaagt gaactgacag ccaaatggag gagtcacgtg aagctataca tttacgatca
    20701 ctgcccttac tgcctcaaag cccgcatgat tttcggcctg aaaaatatcc ccgtcgaatt
    20761 acatgttctg ctcaacgacg acgcagaaac acccacccgg atggtcggtc aaaaacaggt
    20821 tcccattctg caaaaagatg acagccgcta tatgccagaa agcatggata tcgttcacta
    20881 tgtcgataaa ctcgacggca aaccgttact gaccggcaaa cgttcccctg ccattgaaga
    20941 gtggctgcgc aaggtcaatg gctacgccaa caaactgctg ttgccgcgtt ttgccaaatc
    21001 ggcatttgat gagttttcta ctcccgccgc gcgcaaatat ttcgtcgaca agaaagaggc
    21061 cagcgcgggt aattttgccg acctgctggc ccactctgac ggtctgatta agaatatcag
    21121 cgatgatttg cgtgcgctgg acaaactgat cgtcaaaccg aacgccgtga atggagaact
    21181 ttcggaagat gatattcagc tatttccgct actgcgtaat ctgacgctgg tagccggaat
    21241 caactggcca agccgcgttg ctgattaccg cgataatatg gcgaaacaga cacaaatcaa
    21301 tttgttatca tcaatggcga tttaatcctg acccgccgcg gcagcctgta ttcgcgggtt
    21361 gccgccgttt tcagtttttt ctgctggcga gtttatcgct ggtgacacat gctgcgttgt
    21421 gtcaggatat tgacgcagat ttcatgtcga ggactaccat gaacaagttt ttatttgccg
    21481 ctgcattgat cgtcagtggc ctgctcgttg gctgtaatca actcacccaa tacaccatca
    21541 ccgaacaaga aattaaccag tcgcttgcga aacataataa tttctcaaaa gatatcggtt
    21601 tacccggcgt ggctgacgcc catattgttc tgacaaactt gaccagccaa attggtcgcg
    21661 aagagccgaa taaggttacc ctaaccggag acgccaatct ggacatgaac tccctgttcg
    21721 gtagtcagaa agcgaccatg aaactgaagc tgaaagcgct gcccgtgttt gataaagaaa
    21781 aaggtgcgat cttcctgaaa gagatggaag tggtcgatgc gacggtacaa ccggaaaaaa
    21841 tgcaaacggt gatgcaaacg ttgcttccct atttgaacca ggcattacgc aattacttta
    21901 accagcaacc tgcttacgtc ctgcgcgaag atggcagtca gggcgaagca atggcgaaaa
    21961 aactggcgaa aggcattgaa gtgaagccag gcgaaattgt cattccgttt acggattaat
    22021 gacgagggcg cattcgcgcc ctttattttt cgtgcaaagg aaaacgtttc cgcttatcct
    22081 ttgtgtccgg caaaaacatc ccttcagccg gagcatagag attaatgact gcaccatccc
    22141 aggtattaaa gatccgccgc ccagacgact ggcaccttca cctccgcgat ggcgacatgt
    22201 taaaaactgt catgccgtat accagcgaaa tttatggacg ggctattgta atgcccaatc
    22261 tggctccgcc cgtgaccact gttgaggctg ccgtggcgta tcgccagcgt attcttgatg
    22321 ccgtacctgc cgggcacaat ttcaccccat tgatgacctg ttatttaaca gattcgctgg
    22381 atcctaatga gctggagcgc ggatttaacg aaggcgtgtt caccgctgca aaactttatc
    22441 cggcaaacgc aaccactaac tccagccacg gcgtgacgtc aattgacgca atcatgccgg
    22501 tacttgagcg catggaaaaa atcggtatgc cgctactggt gcatggtgaa gtgacacatg
    22561 cagatatcga catttttgat cgtgaagcgc actttataga aagcgtgatg gaacctctgc
    22621 gccagcgcct gactgcgctg aaagtcgttt ttgagcacat caccaccaaa gatgctgccg
    22681 actatgtccg tgacggaaat gaacggctgg ctgccaccat cactccgcag catctgatgt
    22741 ttaaccgcaa ccatatgctg gttggtggcg tgcgtccgca cctgtattgt ttacccatcc
    22801 tcaaacgcaa tattcaccaa caggcattgc gtgaactggt cgccagcggt tttaatcgag
    22861 tattcctcgg tacggattct gcgccacatg cacgtcatcg caaagagagc agttgcggct
    22921 gcgcgggctg cttcaacgcc ccaaccgcgc tgggcagtta cgctaccgtc tttgaagaga
    22981 tgaatgctct gcagcacttt gaagcattct gttctgtaaa cggcccgcag ttctatggct
    23041 tgccggtcaa cgacacattc atcgaactgg tacgtgaaga gcaacaggtt gctgaaagca
    23101 tcgcactgac tgatgacacc ctggtgccat tcctcgccgg ggaaacggta cgctggtccg
    23161 ttaaacaata aaaatcgtag cttcctgttg tcattaggtt attttacctg tataaataac
    23221 cagtatattc aacagggggc tattatgcga attgaagtca ccatagcgaa aacttctcca
    23281 ttgccagctg gggctattga cgccctggct ggcgaacttt cccgccgtat tcagtatgcg
    23341 tttcctgata atgaaggcca cgtatcggta cgttatgccg cagcgaataa tttatcggtt
    23401 attggcgtaa caaaagaaga taaacagcgc attagcgaaa ttctccagga aacgtgggaa
    23461 agcgccgatg actggtttgt cagcgaataa tatgcagtga tttttgtttt tgccgggtcg
    23521 ccccggcttt tttattttac ttacaaattt tgagtacaaa atttctacac ttcctcaaaa
    23581 taattgcaca atatgctaaa aaaatatgaa tgacatgctg ttttattgtt catataaatc
    23641 ttaaaagcag aaatttatct gttcccccct ttaaaaattc ggtgaatacc cttacttatt
    23701 ggtatactga agttgcttca gataaaaacg cattgaacct cgaataacgt tgtctagtaa
    23761 cacgaattag ggggccatga tggaaaaaaa taatgaagtc attcagactc atccgctcgt
    23821 agggtgggac atcagcaccg ttgatagcta tgatgcgctg atgttgcgtt tgcactacca
    23881 gaccccaaat aagtccgagc aggaagggac tgaagttggt cagacgctct ggttaaccac
    23941 tgatgttgcc agacaattta tttcgatatt agaagcagga atcgccaaaa ttgaatccgg
    24001 tgatttcccg gtaaacgagt atcggcgtca ttaacatgac atgaccatcc acccaaaagg
    24061 cacctcaccg gtgcctttac catttctggg ttgtatatct gcattcagtt aattactctg
    24121 cttaaaccat gagagcagag agaaaaaaat gaaatacgat ctcatcatta ttggcagcgg
    24181 ttccgtaggc gctgccgccg ggtattatgc aacccgcgcc ggtttaaacg tgctaatgac
    24241 cgacgcccat atgccaccgc atcaacacgg cagccaccac ggcgatacgc gattaattcg
    24301 ccatgcttat ggtgaaggcg aaaagtatgt cccgttggtc ctccgcgcgc aaacgctgtg
    24361 ggatgaactt tcccgccaca acgaagatga tcccattttt gtacgctctg gtgtcattaa
    24421 cctcggcccg gctgactccg catttctcgc caacgtcgcc cacagcgccg aacagtggca
    24481 actcaacgtt gaaaagctcg atgcgcaagg gattatggcc cgctggccag aaatacgcgt
    24541 cccggacaac tacatcggct tatttgagac tgattccggt tttttgcgca gcgaactggc
    24601 gattaaaacc tggatccaac tggcgaagga agcgggctgt gcgcaactgt tcaactgccc
    24661 ggtcaccgca attcgtcatg acgatgatgg cgtaactatt gaaaccgttg acggtgagta
    24721 tcaggcgaaa aaagcgattg tctgcgcggg aacatgggta aaagacctgc tcccggagct
    24781 gcctgtccag cctgtacgta aagtatttgc ctggtatcag gccgatggcc gctatagcgt
    24841 gaagaataaa ttcccggcgt ttaccggtga actgcccaat ggcgatcaat attatggttt
    24901 tccggcagaa aacgacgcgt tgaagattgg caaacataac ggaggccagg ttatccattc
    24961 agcggatgaa cgtgttccgt ttgcggaagt ggtcagcgat ggttcggaag ccttcccgtt
    25021 cttgcgcaat gtattgccgg gtatcggttg ctgcctgtac ggcgctgcct gcacctatga
    25081 taattcgcct gacgaagatt ttattatcga taccctaccc ggccacgata atacactgct
    25141 cattaccggc ctgagtgggc acggttttaa atttgcgtca gttttagggg aaatagctgc
    25201 cgattttgcg caagacaaaa aaagcgattt tgatttgacg ccattcaggc tttcccgctt
    25261 ccaataatca tcattacggc ctccagaatg aggccgtttt tacaggagta tttaatgcgt
    25321 cggctgttgc actatctcat caataatatt cgcgagcatc tgatgcttta tctttttctc
    25381 tggggattgt tggcaattat ggatctgatc tatgtgtttt atttttagtt ttccgcactg
    25441 acataaactg acaaatattt aatctccctt tcatttagcg tgaaaaattg aatgagatta
    25501 ttcctgcttc tacagcactc accagacatc tctgatgctg aacccactaa acttatgaag
    25561 attctttcat tattaattca aaattattga aataaaaata aatatcattc aatttgttat
    25621 ttttgtgttg caattcattc tccgacacgc cattttcttt ctctcgcaaa ttcgtattgg
    25681 atgcacttat gtcattcaca aatacccctg aacgctatgg tgttatatct gcggccttcc
    25741 attggctaag cgcaattatt gtctacggca tgtttacttt aggtctgtgg atggtcacgc
    25801 tcagttatta cgatggctgg tatcacaaag cacccgaact gcataaaagc atcggtattt
    25861 tgttaatgat ggggctggtt attcgcgttc tgtggcgtgt catatctcca ccgccgggtc
    25921 cgctgccgag ttattcgccc atgactcgtc ttgccgcaaa ggctggacat ctcgcattgt
    25981 acctattgct gttcgccatc ggcatcagcg gctatctgat ctcaactgcc gatggtaaac
    26041 cgatcagcgt ttttggctgg tttgacgtcc ccgcgaccct tgccgacgct ggcgcacagg
    26101 cagactttgc cggtgccctg catttttggc tcgcgtggag cgtcgtagta ctgtccgtta
    26161 tgcacggatt tatggccctg aagcatcatt tcatcgataa agacgacact ctgaagcgca
    26221 tgctgggaaa gtcgtcatct gactatggag tataaaaaat gaaaaaaagc ctgcttggtt
    26281 taaccttcgc gtccctgatg ttctctgccg gttcagcggt tgccgccgat tacaaaattg
    26341 acaaagaagg tcagcacgcc tttgttaatt tccgcatcca gcaccttggc tatagctggt
    26401 tatacggcac ctttgaagat ttcgacggta cttttacctt tgacgaaaaa aatccggctg
    26461 ccgataaagt gaatgtgaca attaacacca ccagcgtcga tactaatcac gccgaacgcg
    26521 ataaacatct tcgcagtgca gatttcctca ataccgcaaa atatccacag gcaacattca
    26581 cctccaccag cgtgaagaaa gacggtgacg aactggatat taccggcgat ctgacgctga
    26641 atggcgtaac caaacccgtc acgctggaag cgaaattaat tggtcagggc gacgacccat
    26701 ggggtggtaa acgtgcaggc ttcgaggccg aaggcaaaat taagctcaag gacttcaata
    26761 tcaagacaga tttaggtcca gcttctcagg aagtggatct gattatttca gtggaaggcg
    26821 tacagcagaa gtaagcgtga aatgttgccg gatggcacgc atccggcaat gatatttatt
    26881 ctgttggatc aggaatgccc agtgttgtat tcagacgtcc acgagactta ttaaagatct
    26941 tattgccatt ttcacgtcct gcccgacggc gtcgctgttc ttctggcggt aacgcgcttt
    27001 cttcgcagca aatctcacta caacaacctt tgtatttttc cgcgcatact ggacactgaa
    27061 taaacagcag gtggcagcca tcatttttac agttggtatg gctgtcgcac ggcgcgccgc
    27121 actggtggca atgcgcgata atctcatccg atatacgttc gcccatccgc tcgtcaaaaa
    27181 caaaattttt gccaataaaa cgcaccggca agccctgctc gcgcgcctta cgggcgtatt
    27241 caataattcc gccctcgata tgccagactt tgttgaatcc gttatgtttc atccaagcac
    27301 tggccttttc acaacgaatg ccgccggtgc agtacatgac gattttttta tctttatgtg
    27361 cctgcatcat ctcgactgct tttggcagct gctcacggaa ggtatctgcc ggaatttcga
    27421 gcgcgttttc aaagtgcccc acttcatact catagtggtt acg
//
LOCUS       DAJVUJ010000041        27206 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.041, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000041 DAJVUJ010000000
VERSION     DAJVUJ010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27206
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.041"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(40..1338)
                     /gene="eno"
                     /locus_tag="OQQ48_001593"
     CDS             complement(40..1338)
                     /gene="eno"
                     /locus_tag="OQQ48_001593"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCS1952404.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(1426..3063)
                     /gene="pyrG"
                     /locus_tag="OQQ48_001594"
     CDS             complement(1426..3063)
                     /gene="pyrG"
                     /locus_tag="OQQ48_001594"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HCS1952405.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(3291..4082)
                     /gene="mazG"
                     /locus_tag="OQQ48_001595"
     CDS             complement(3291..4082)
                     /gene="mazG"
                     /locus_tag="OQQ48_001595"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HCS1952406.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4153..4488)
                     /gene="mazF"
                     /locus_tag="OQQ48_001596"
     CDS             complement(4153..4488)
                     /gene="mazF"
                     /locus_tag="OQQ48_001596"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HCS1952407.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKRIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(4488..4736)
                     /gene="mazE"
                     /locus_tag="OQQ48_001597"
     CDS             complement(4488..4736)
                     /gene="mazE"
                     /locus_tag="OQQ48_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HCS1952408.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(4814..7048)
                     /locus_tag="OQQ48_001598"
     CDS             complement(4814..7048)
                     /locus_tag="OQQ48_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HCS1952409.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7096..8397)
                     /gene="rlmD"
                     /locus_tag="OQQ48_001599"
     CDS             complement(7096..8397)
                     /gene="rlmD"
                     /locus_tag="OQQ48_001599"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCS1952410.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     SALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8454..11210
                     /gene="barA"
                     /locus_tag="OQQ48_001600"
     CDS             8454..11210
                     /gene="barA"
                     /locus_tag="OQQ48_001600"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HCS1952411.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11442..12782)
                     /gene="gudD"
                     /locus_tag="OQQ48_001601"
     CDS             complement(11442..12782)
                     /gene="gudD"
                     /locus_tag="OQQ48_001601"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCS1952412.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIMIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGDRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWLLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLCARDNAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12803..14143)
                     /locus_tag="OQQ48_001602"
     CDS             complement(12803..14143)
                     /locus_tag="OQQ48_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCS1952413.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELHVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14145..15515)
                     /gene="gudP"
                     /locus_tag="OQQ48_001603"
     CDS             complement(14145..15515)
                     /gene="gudP"
                     /locus_tag="OQQ48_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HCS1952414.1"
                     /translation="MFRSATTSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRAT
                     LSIAGSEMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWS
                     MFTLLQGFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSI
                     FNSAQYFATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQ
                     KELEYIAAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYF
                     FITWFPVYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKT
                     PIVMGMLLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSG
                     GLFNMFGNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIEL
                     KPVAGQ"
     gene            complement(15932..16381)
                     /locus_tag="OQQ48_001604"
     CDS             complement(15932..16381)
                     /locus_tag="OQQ48_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HCS1952415.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16399..17181)
                     /gene="truC"
                     /locus_tag="OQQ48_001605"
     CDS             complement(16399..17181)
                     /gene="truC"
                     /locus_tag="OQQ48_001605"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HCS1952416.1"
                     /translation="MLEILYQDEWLVSVNKPSGWLVHRSWLDRDEKVVVMQTVRNQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17181..17510)
                     /locus_tag="OQQ48_001606"
     CDS             complement(17181..17510)
                     /locus_tag="OQQ48_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HCS1952417.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQHALILAELEKLDA
                     LFADDAS"
     gene            complement(18132..18677)
                     /gene="syd"
                     /locus_tag="OQQ48_001607"
     CDS             complement(18132..18677)
                     /gene="syd"
                     /locus_tag="OQQ48_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HCS1952418.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            18745..19593
                     /gene="queF"
                     /locus_tag="OQQ48_001608"
     CDS             18745..19593
                     /gene="queF"
                     /locus_tag="OQQ48_001608"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HCS1952419.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATCGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19705..21069
                     /gene="ppnN"
                     /locus_tag="OQQ48_001609"
                     /gene_synonym="ygdH"
     CDS             19705..21069
                     /gene="ppnN"
                     /locus_tag="OQQ48_001609"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HCS1952420.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            21626..22915
                     /gene="sdaC"
                     /locus_tag="OQQ48_001610"
     CDS             21626..22915
                     /gene="sdaC"
                     /locus_tag="OQQ48_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HCS1952421.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            22973..24340
                     /gene="sdaB"
                     /locus_tag="OQQ48_001611"
     CDS             22973..24340
                     /gene="sdaB"
                     /locus_tag="OQQ48_001611"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HCS1952422.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            24452..25207
                     /gene="xni"
                     /locus_tag="OQQ48_001612"
     CDS             24452..25207
                     /gene="xni"
                     /locus_tag="OQQ48_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HCS1952423.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(25312..26449)
                     /gene="fucO"
                     /locus_tag="OQQ48_001613"
                     /pseudo
     CDS             complement(25312..26449)
                     /gene="fucO"
                     /locus_tag="OQQ48_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
     gene            complement(26477..27124)
                     /gene="fucA"
                     /locus_tag="OQQ48_001614"
     CDS             complement(26477..27124)
                     /gene="fucA"
                     /locus_tag="OQQ48_001614"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HCS1952424.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIAFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
BASE COUNT         6581 a         7297 c         6553 g         6775 t
ORIGIN      
        1 tcccggaggc tggcattttt aaatcagata aagtcagtct tatgcctggc ctttgatctc
       61 tttacgaccg ttgtacggtg ctttttcgcc cagagcttct tcgatacgaa tcagctggtt
      121 gtatttagca acacggtcag aacggctcat agaaccagtt ttgatctggc ctgcagcagt
      181 accaacagcc aggtcagcga tggtagcgtc ttcagtttcg ccagaacggt gagagataac
      241 tgcagtgtag ccagcatctt tcgccatctt gattgcagcc agagtttcgg tcagagaacc
      301 gatctggttg aatttgatca ggatggagtt agcgatacct ttttcgatac cttctttcag
      361 gatcttggtg ttggttacga acaggtcgtc accaaccagc tggattttgt cgcccagaac
      421 tttggtctgg tatgcgaaac cgtcccagtc agattcgtcc agaccgtctt cgatagaaac
      481 gatcgggtac tgtttggtca gttcttccag gaagtgagtg aattcttcag aggtgaacgc
      541 tttgttgcct tcgccagcca gaacgtattt accatctttg tagaattcag aagctgcgca
      601 gtccatcgcc agagtgatgt ctttgcccag ttcataacca gcagctttaa cagcttcagc
      661 gataacagcc agagcttcag cgttggaacc caggttcggc gcatagccac cttcgtcacc
      721 aacagcagta ttcatgcctt tcgctttcag aacttttgcc aggtgatgga aaacttcaga
      781 acccatgcgg atggcttctt tcacagtttt cgcgccaacc ggctgaatca tgaattcctg
      841 gatatcaacg ttgttgtcag cgtgctcacc accgttgatg atgttcatca tcggaaccgg
      901 catagagtat ttgcccggag taccgttcag ttcagcgatg tgctcgtaca gcggcatacc
      961 tttagcagct gcagcagctt tggcgttagc cagagataca gccaggattg cgttcgcgcc
     1021 gaatttggat ttgttttcgg tgccgtccag gtcgatcatg atcttgtcaa tgccagcctg
     1081 atctttagca tctttgccaa tcagcgcctg agcgatcggg ccgtttaccg cagcaacagc
     1141 tttggttacg cctttaccca ggaaacggga tttgtcgcca tcgcgcagtt ccagagcttc
     1201 acgggaacca gtagaagcac ctgacggagc agctgccata ccgacgaaac caccctccag
     1261 atgtacttcg gcttcaacag tcgggttacc acgggagtcg atgatttcac gaccgatgat
     1321 ttttacgatt ttggacatta ggttttcctc aagtcactag ttaaactgaa actccagaca
     1381 aacaacacgt acccagggta cgcgttgccg ttctaacttt tttacttact tcgcctgacg
     1441 tttctggaac tcgctggcgg ctttcacaaa gcctgcaaac agcgggtgac catcacgtgg
     1501 agtagaagta aactccggat ggaactggca agccacgaac cacgggtgat tcggaacttc
     1561 gatgatctcg accaactgat catccccgga acggcccgca acgcgcagac ctgcatcttc
     1621 aatctgtttc aacagcatgt tgttgacttc gtaacggtga cgatgacgct caacaattgt
     1681 cggcgcattg tacagctggc gaaccaggct atcgtcaacc aactggcact gctgtgcgcc
     1741 gagacgcatg gtaccgccga gatcgctctt ctcgctacga acttcaacgt tgccgttttc
     1801 atcgcgccac tcggtaatca gcgccacaac cgggtactta cagtctggca caaattccgt
     1861 agagttggcg ttctccatgt tggcaacatg gcgagcgtaa tcaattaacg ccacctgcat
     1921 acccaggcaa atgcccagat aaggaatatt gttctcacgc gcaaaacgcg cggtcgtaat
     1981 catgccttct acgccacgat agccgaaacc gccaggtacg aggattgcgt ccagaccttt
     2041 aaggatttca acgccgcgcg tttcaacatc ttgtgaatcg atcagtttga tgttgacgct
     2101 gacacgattc ttcagcccac cgtgtttcag tgcttcgatc actgatttat aagcatccgg
     2161 cagttcaatg tacttgccga ccataccgat ggtgacttca cttaccgggt tcgcttcttc
     2221 gaagataacc tgttcccatt cggacagatt cgcttccggg cagtttaagc tgaatcgttt
     2281 acaaatataa tcgtccagcc cctgagattt caacaggccc ggaattttat agatggaatc
     2341 gacgtctttc agagaaataa ccgctttttc cggaacatta cagaacaatg caatcttcgc
     2401 acgttcgttc gccggaacag cgcgatctga acgacaaatc aggatgtcag gctggatacc
     2461 gatggagagc agctctttta cagagtgctg agtcggtttg gttttgactt caccagacgc
     2521 tgccatgtac ggcaccagcg tcaggtgcat aaacagagtg tgctcacggc caatttcaac
     2581 agccatctgg cgaatcgctt cgaggaacgg caaggattcg atatcaccta ctgtaccgcc
     2641 gatttctacc agtactacgt catgaccttc gccaccttcc agcacgcgct ctttgattgc
     2701 gttagtgatg tgcggaataa cctgcacggt tgcgccgagg tagtcaccgc ggcgttcttt
     2761 acgcagaacg tcagagtaga tacgacccgt ggtgaagttg ttgcggcggc tcattttggt
     2821 acgaatgaaa cgctcgtagt gccccaggtc caggtcggtt tcagcgccgt cttcagtaac
     2881 gaacacttcc ccgtgttgga ttgggctcat agtacctgga tcgacgttga tgtacggatc
     2941 cagtttcatg atggtcacat tgaggccacg ggcttcaaga atggctgcga gggaggctgc
     3001 ggcaatgcct ttacccagag aggatacgac cccgccggtc acaaaaatat agttcgttgt
     3061 catgctgaac ctgagaagtt aggttgaaaa gacgatggaa taaccaggac gggaaagcag
     3121 tatacccgaa catgacctgt gccacaaact ttcattatcc ctcctcttcg ccagcgcact
     3181 attgaaatca ggagtgagaa aatagcccct ttggggtaaa tgtttttgac gcaaatcaag
     3241 cgcttgtcat ttaaaaaatg acacaaatgg cacttgaccg cgtaattccc ttagagatca
     3301 atttcctgcc gttttacctg ttgccagact tcttccattg tttcgaggtc aacacctgtc
     3361 atttccagtc cacgcgcggc aacaatacgc tccacttcgc gaaaacgacg ctcgaatttt
     3421 tcgttcgctt tttgcaatgc gatttctgct ttcgtcccta aatggcgagc cagattaacc
     3481 gtggcaaaca gcaggtcccc catttcctcc tccagtttag cctggtcgac aacagcctgc
     3541 cgcgcttcgt acatcacctc gtcgatctct tcgtagactt tatcaaccac cgggccaagc
     3601 gtcgtccagt cgaagccaac gttggcgcaa cgtttctgga ttttttgcgc ccgcattaaa
     3661 gccggtaaac tacgaggaat atcgtccagc gccgaatgct gcgctttctg cgcgcgctct
     3721 tcggttttga tttgctccca acgggcaagc acttcactac tgttttcggc agaactatca
     3781 gcaaaaacat gcggatggcg acgctctaat ttatcgctaa tagcagcgca aatatcatta
     3841 aagtcaaagc gcccttcttc ctgagccatt tgcgcgtaaa acaccacctg gaatagcaga
     3901 tcgcccagtt caccgcgaag atcgtcaaaa tcttcacggg cgatggcgtc cagcacttcg
     3961 taggtttctt caagggtgta aggcgcaatg gtggcaaatg tctgctcttt atcccacggg
     4021 cagccgtttt ccggatcgcg caggcgctgc ataatagtga gcaaacgatc gatttgattc
     4081 attgaattgt cctgaaaatt gcgggtctgt caggtggaaa cctgtgacca gaatagaagt
     4141 gagttagtaa cactacccaa tcagtacgtt aattttggct ttaatgagtt gtaattcctc
     4201 tggggcaact gttcctttct tcgttgctcc tcttgcccgc caggcgattc tttttacctg
     4261 atcagctaac gctacgccat cacgttcctg accggataaa acaacttcga acggatatcc
     4321 ttttgattgc gttgtacaag gaacacacag acacatacct gttttgttgt tgtacatgaa
     4381 aggactcagg acaacagctg gacgatgtcc agcttgctcg ctaccttttg tcgggtcaaa
     4441 atcaacccaa atcagatcgc ccatatcggg tacgtatcgg cttaccatta ccagacttcc
     4501 ttatctttcg gctctcccca gtcgatattc tcgtggaggt tttccggcgt gatgtcgttg
     4561 accagttcag caagcgtaaa tacgggctct ttacgcactg gctcaataat taatttgcca
     4621 tccaccaggt caatcttcac ttcatcatca atattcagat tgagcgcctg cattaacgta
     4681 gccgggatcc gcaccgccgg tgaatttccc caacgcttta cgctactgtg gatcatcacc
     4741 ctttcctcaa accgctatca tatgtagata cagtatatat caatctacat tgtagatacg
     4801 agcaaatttc tgcctaactc ccgtgcaacc gacgcgcgtc gataacatcc ggcacctggt
     4861 tgagtttacc cagcacgcgg cccagcacct gcaggttgta aatctcaatg gtcatgtcga
     4921 tggtcgccag ttgctgtttg gtgtcgctac ggctggcaac gccaagtacg ttcaccttct
     4981 cgttggcgag aatggtcgtg atatcacgta acaacccact acgatcatta gccaccacgc
     5041 ggaccaccag cgaatatccg gcggagtagc tctcacccca taccgcgtca acaatgcgtt
     5101 ctggcgcatg agagcgcagt tccgccagtt gttcgcaatc ggcgcggtgt actgaaatac
     5161 cgcgcccctg ggtaatgaag ccgacaatct catctccagg aatcggctgg cagcagcgcg
     5221 cgatgtggtg catcaggttg ccaacacctt cgactaccac gcgaccgtta tctttactgc
     5281 ggttttgcgg cgtgtagctt ttttgctgaa gttgcttcag cgcggcggcg tcctgctctt
     5341 cggcactcgg cttattaaat tgcgattgca ggaagttcac catctgattg agacggatat
     5401 ccccgccacc aatcgccgcc agcaactcgt cgacatcatt aaagttgtaa cgcggcagca
     5461 gatgtttttc tgcttctttc aggctgatcc ccagatgttc cagctcgtcg tcaaggattt
     5521 gccgcccagc cagaatgttt ttgtcacggt cctgtttacg gaaccaggcg tgaattttcg
     5581 aacgcccacg gctggttgtg acgtaaccga ggtttgggtt taaccagtca cggctggggt
     5641 tcggctgttt ctgggtgata atttcaatct ggtcgcccat ctgcagctgg taggtgaacg
     5701 gcacaatgcg cccgccaatt tttgccccga tgcagcggtg tccgacatca ctgtggatgt
     5761 ggtaagcgaa gtccagcggc gttgatcccg caggcaaatc aacgacatca cctttcggcg
     5821 taaagacata cacccggtcg tcaaagacct ggctgcgtac ttcgtcgagc atttcgccgg
     5881 aatcagccat ctcttcctgc cacgcaatca gtttacgcag ccaggcaatc cggtcttcat
     5941 gtcccgaacg tgcgccgcca gctgccgcgc cctctttata tttccagtgc gcagcaacac
     6001 ccaactctgc atcttcatgc atctgtttgg tgcggatttg gatctcaacg gttttgccac
     6061 ctggccccag aaccacggta tgaatagact gataaccgtt tggtttcggg ttagcgacat
     6121 aatcgtcaaa ctcatccggc aggtggcgat agtgagtgtg cactatcccc agtgcggcat
     6181 aacaatcctg taaacgctcg gcgacaatac gtaccgcacg cacatcaaac agctcatcga
     6241 aggcgaggtt ctttttctgc attttgcgcc agatgctgta gatgtgtttc ggacggccat
     6301 acacttcagc tttaacgcct tcagctttca tctcagcgcg cagatgaccg acaaactctt
     6361 cgatatagtg ttcgcggtcg agacggcgtt catgcagcag ttttgcgatg cgtttgtatt
     6421 cggtcgggtg cagataacgg aagcagtaat cttccagctc ccatttcagt tgcccaatcc
     6481 caagacggtt cgccaacggc gcgtagatat tggtgcactc ttttgccgcc agtacgcgtt
     6541 catcttccgg cgcatctttt acttcgcgca gatgagcaat acgctccgcc agtttgatga
     6601 ccacgcagcg aaaatcatcg accatcgcca ataacatccg gcgaacgtta tcgacctgtt
     6661 cggaggaaac agaatcagtg tgcgtcgctt tcagctggcg gatcgccgcc atatcacgca
     6721 cgccgtgaat aaggttaacg acagacttac cgacgctctc acgcagcaca tcttcgctga
     6781 ctacgttggc atcggccaga gggaacagca gcgccgcccg cagcgtgtca atgtccatac
     6841 ttaatgtcga gaggatctcc accatctcaa caccacgcca caataacaga ctggcatccg
     6901 gatgcccctg cgtctgctgc agacaatacg cccaggtttc ggctaagcac tcacacgact
     6961 tctggctggt aatacccaga cttgcgatcc atttttccgg atcaaattca ccagccttat
     7021 tgatatgtgc acttcttacc gcaaccattg tcctctcctt tagggaccag acctgccgaa
     7081 atcggcaaat cgtaactatt taacgcgcga gaaaagtacc atcgattcca gatgtcccgt
     7141 gtgtgggaac atatccagca tcgccagtcg cgcaatggta tatcctgctt ttaataacgc
     7201 ttcgctatcc cgagccagcg ttgcagggtt acaggataca taaactatac gaataggttc
     7261 cagttttata atttgctgca taacacctgc ggcacctgct cgcgccgggt ccagcaacac
     7321 tttatcgaag ccgtttttcg cccacggctg ctttgtgaca tcttcttcaa gattttcgtg
     7381 ataaaacgtc acattctgta agccattaag acgcgcattc tgctggcctt tttccaccag
     7441 cgccggaaca ccttctacac cgaccacact ggcagcttgt gtcgccaatg gcagtgtaaa
     7501 gttgcccata ccgcagaaca gatccagtac gcgatcttca ggttgcacat ccagccattc
     7561 caacgcactc gctaccattt tttggttcac acccgcattg acctgaataa aatcgcgcgg
     7621 gctaaaagtt aagcgcaacc cgttagagtc ataccagggc atctcaccag agacggtttc
     7681 gagtatctca ctatcggggg cgagatacag atccaggcct tcagaatgcg aaaagcgttc
     7741 cagtttttcg cgatctgccg aacttagcgg tgcggtatgg cgcaaaatca tcagcgtgcc
     7801 gctggttgcc tgtaccagtt caacatgacc aagatggcgc attgcttgta agctgcccag
     7861 gcatgccctg actttgggca gcaatgcttc aagttggggc actaaaatgg gacattgttt
     7921 aacgtcgaca atgtcactgg agcccgcttt gcgaaacccc atctgaagtt gctgtgtttt
     7981 cggtaagtag ttcagactta aacgcgcgcg acggcgatag ccccagggaa catcggcgat
     8041 cacttcagag acttcgtgtt tcattaatcg ggcgagtgcc gcacttttgc ttcgctgctg
     8101 taaatccacg ctggcgtgtt gttgctgaca gccgccgcat acgccaaaat gaggacagcg
     8161 tggcgtttcg cgttccgggc tatcgcttaa ccggcgtacg actttagcgc gggcatactg
     8221 ttttttatct tcagtaacag taacttccgc gttttcctgc ggcaataatc cggggataaa
     8281 tagcgttttg ccgttatgtc gcgccacgcc ctgaccaaaa gagtcgaggt cgttgactga
     8341 aacggttatg atctgacgcg tcgtcgtgcg tcgttttgca gagtagaatt gcgccattgg
     8401 cgagactttc tcaatttaac agtgtgacct taattgtcac ataacggaac tccatgacca
     8461 actacagcct gcgcgcacgc atgatgattc tgatcctggc accgaccgtc cttattggtt
     8521 tattgctgag tatatttttc gtcgtgcatc gctataacga cttgcagcgt caactggaag
     8581 atgcaggtgc cagcattatt gagccgcttg cagtttctac tgaatatggc atgagcctgc
     8641 aaaatcgcga atctatcggt cagttaataa gcgtactgca tcgtcgccat tccgatattg
     8701 ttcgcgcgat ttcggtttat gatgaaaata accgactctt tgtcacctcc aattttcatc
     8761 ttgatccctc atcaatgcag ctcggcagca acgtgccgtt tcctcgccag ctcactgtca
     8821 ctcgtgacgg cgatattatg atcctccgca cgccgattat ttctgagagt tactcccccg
     8881 acgaatcgcc cagtagcgat gccaaaaata gtcaaaatat gttgggatat attgcgcttg
     8941 agctggatct taaatcggtt cgcttgcagc aatataaaga gatctttatt tccagcgtga
     9001 tgatgctgtt ttgtatcggt attgcgctta tttttggctg gcgcttaatg cgcgatgtaa
     9061 ccggtccgat tcgcaacatg gtgaataccg tcgaccgcat ccgtcgcggg caactcgaca
     9121 gccgggtgga aggatttatg ctcggcgagc tggatatgct gaaaaacggt atcaactcga
     9181 tggcaatgtc gctggctgct tatcacgaag agatgcagca caatatcgac caggcgacgt
     9241 ccgatctgcg tgaaacgctg gagcagatgg aaattcagaa cgttgagttg gatctggcga
     9301 aaaagcgcgc ccaggaagcg gcgcgtatta aatccgagtt tctggcaaat atgtcacacg
     9361 agctgcgtac accactgaat ggtgttattg gctttacccg cctgacgctg aaaacagaat
     9421 taacaccaac gcagcgcgat cacctgaata cgattgaacg ttcggcaaat aatttgctgg
     9481 caattattaa tgatgttctc gacttctcga aactggaagc aggtaagctg attctggaaa
     9541 gtattccatt cccactacgc agcacgctgg atgaagtcgt tactctgctg gcacattctt
     9601 ctcacgataa agggttggaa ctgacgctca atattaaaag cgacgtgcct gataacgtga
     9661 tcggcgaccc actgcgatta cagcaaatca tcactaacct ggtggggaat gcaattaaat
     9721 tcaccgagaa tggcaacatt gatattctgg tagaaaaacg tgcgctgagt aataccaaag
     9781 tgcagattga agtgcagatt cgggataccg gcattggtat tcctgaacgc gatcaatcgc
     9841 gcttattcca ggccttccga caggctgatg ccagtatttc ccgccgtcat ggtggcaccg
     9901 ggctggggct ggtgattaca caaaaactgg ttaatgaaat gggcggcgat atttcgttcc
     9961 atagccagcc gaatcgcggt tcaactttct ggttccacat taatctcgat ctgaacccga
    10021 acattattat cgaagggcca tccacccagt gcctcgcagg taagcgcctg gcctatgtcg
    10081 aaccaaactc cgcagcagcg caatgcacgc tggatatttt aagtgaaacg ccgctggaag
    10141 tggtttatag cccaacgttc tccgcgctgc ctcccgcgca ttacgacatg atgttgttag
    10201 gcatcgcggt gaccttccgc gagccgctaa caatgcaaca tgagcgatta gcgaaagcgg
    10261 tatcgatgac cgatttcctg atgctggcac ttccttgcca tgcacaagtc aatgctgaaa
    10321 aactcaagca agatggtatc ggcgcgtgtc tgctgaaacc attaacacct acacgcctgt
    10381 tgcctgccct gacggaattt tgtcatcaca aacaaaacac gcttttgcct gtaaccgatg
    10441 aaagtaagct ggcaatgaca gtcatggcgg ttgatgacaa ccccgctaac ctgaaactta
    10501 tcggcgcatt gctggaagat atggtgcaac atgtggaact ttgcgatagc gggcatcagg
    10561 cggttgaacg ggcgaaacag atgccgttcg atttgatctt aatggatatt caaatgccgg
    10621 acatggatgg cattcgggcc tgcgaactca tccaccagct cccccatcag caacaaacgc
    10681 cggttatcgc ggtaacggcg catgcaatgg ccgggcaaaa agagaagctg cttggcgcag
    10741 ggatgagcga ttatctggcg aaaccgattg aagaagagcg attgcataat ttgttgttgc
    10801 gctacaagcc tggcagcggt atttcctctc gcgtcgtgac gcccgaagtc aacgaaattg
    10861 tggtgaaccc gaatgcgacc ctcgactggc aactggcact acgccaggca gcaggaaaaa
    10921 ccgatttagc gcgcgatatg ctgcaaatgt tactcgattt cctgcctgaa gttcgcaaca
    10981 aagttgagga acagctggtt ggagaaaacc cggaaggcct ggttgatttg attcataaac
    11041 tgcatggcag ttgcggctat agcggtgtgc cgcgtatgaa gaatctctgc caactgatcg
    11101 aacaacagct acgtagcggt actaaagaag aagatttgga gccggagctg ctggaactgt
    11161 tggacgagat ggataatgtc gcgcgcgaag ccagcaaaat tctcgggtaa cggacgtctt
    11221 gtcgagtagc aatcggcttt ttatgacgct ggtttcagat tggtgacaaa gtgcctgtgt
    11281 ttatgccgga tgcggcgtta acgccttatc cggcctacaa aaccaattaa attcaatgaa
    11341 ttaaaaaatg atgtagacct gatacatagc acaacaggct attttgcgtt tagcatcagt
    11401 ctcaagccgg ctccagatag agccggtttt ggttttctgt cttaacgcac catgcacggg
    11461 cgcttgttat cgaacgtcca gccaggaatc agatactgca ttcccatcgc attgtcacgc
    11521 gcgcaaagcc cgtgtttctg atacagctca tgggctttca tcacttgatc catatcgatt
    11581 tctacaccca gccccggttt ttccggcacc tgaaccagac cgcctttgat ctcaaacggt
    11641 tctttggtca agcgctgatt gccttcctgc caaatccagt gcgtatcaat cgcggtgatt
    11701 ttacccggtg cagcggcggc aacatgggta aacatcgcca gggaaatatc gaagtggttg
    11761 tttgagtgtg aaccccaggt caggccaaat tcatggcaca tttgcgccac acgtaccgaa
    11821 ccttgcatcg tccagaaatg cggatccgcc agcgggatat caacggattg cagggagagc
    11881 gtatggccca tctgccgcca gtcggtggcg atcatattgg ttgcggtcgg caggccagtt
    11941 gcgcgacgga actctgccat cacttcacgc ccggagaaac cttgctccgc accacacgga
    12001 tcttctgcat aagccagcga ccctttcagg tatttaccaa ttttaatcgc ttcgttcagc
    12061 aaccaggcac cgttaggatc gagcgtaata cgcgcctgcg ggaagcgttg cgccagtgcc
    12121 acaatagact cggcctcttc ttccccggcc agtacaccgc ctttcagttt gaaatcgttg
    12181 aagccatatt tttcatacgc cgcttccgcc aggcgcacca ccgcatccgg cgtcatcgct
    12241 tcttcatgac gcaggcgata ccagtcgcat gagtcatccg gctggctttg atacggcagc
    12301 ggtgtggctt tgcgatcacc gacgaagaac agataaccga gcatttcgac ttcgctacgc
    12361 tgttgaccat cgcccagcag ggatgccacg tttaccccca gatgctgccc cagcagatcc
    12421 agcattgccg cttctatccc ggtaactaca tgaatagtgg tacgcaaatc aaatgtctgc
    12481 aaaccgcgcc caccagcatc acgatcggca aaagtattac gcaccagcgt cagaacgttt
    12541 ttgtattcac ccagcgtttt acctaccacc agcggaatcg catcttccag cgttttacgg
    12601 attttctcgc cgccgggaat ttcccctacg ccagtgtgac cagaattatc tttgataatc
    12661 ataatattac gcgtaaagaa cggtgcgtgt gcaccactca gattcatcag catactgtca
    12721 tgacccgcca ccgggatgac ctgcatttca gtaacaacag gcgtcgtaaa ttgagaactc
    12781 atatttatgt cctttttcag aatcaatgac ggccgaaaac gggacgttta cggtcaaagg
    12841 tccagccggg gatcaggtac tgcatcggac ctgcgtcgtt acgcgcaccg ccaggcagac
    12901 gtttataggc ctcatgtgcc ttttgtacct gttcccagtc cagttccacg cccagaccgg
    12961 gcgcatcagg aacggcaatt tttccgtttt taatctccag cggatttttg gtcaggcgac
    13021 aatcgccctc ctgccaaatc cagtgggtat cgatagcggt aggattaccc ggtgccgccg
    13081 cgcccacatg ggtaaacatc gccagagaga tatcgaaatg gttattagaa tggcagcccc
    13141 aggtcagccc ccagtcgtcg caaagctgcg ccacacggac tgcaccggaa agcgtccaga
    13201 agtgcggatc ggcaagtgga atatctaccg cattgagcat caccgcatga cccatttcgc
    13261 gccagttggt ggcgatcatg ttagtcgcga cgggcaagcc ggtcgcccgc cgaaactccg
    13321 ccataacttc acgaccggag aaaccctgtt ctgcgccgca tggatcttcg gcataggtaa
    13381 gaacatcatt cagccctttg catagagaaa tggcttcatc aagtagccag gcaccgttgg
    13441 gatcaacggt aatccgcgca tcagggaagc gtttcttcaa tgcacgaaca gtgtcgattt
    13501 cttgctcgcc aggtaacacc ccgcccttaa gtttgaaatc tttaaagcca tagcgatcct
    13561 gtgaggcttc cgccagacgc acaacggctt cgctgttcat cgctttctga tggcgcaact
    13621 gataccactc atggttgccc ggcgtatttt ccagataagg aagatcggtt ttggtccgat
    13681 caccgatata aaacagataa ccgaggacgg taatagtctc gcgttgcttg cctggcccca
    13741 acagttcgca gaccggaaca ttcagcgcct tacctagcag gtcaagcaag gcggcttcca
    13801 gcgccgccac ggcattaacg tgcaattcaa aagtccaggc acctttgccg aaggtatcaa
    13861 aatcggctgc ctgattacct ttatgcacct gctgaaccac tttattcagg cgcgcaactt
    13921 cctggcccag aaccatcgga atagcatcga ccagtgtttg ataaatcacc tctccgcccg
    13981 gcgcttcacc aatgccggta tgcccggcgt tatcggtgag taccacaata ttgcgggtga
    14041 aatatgcgtt atgtgcccca ccaatattaa gcaacatgct atcgtgcccg gcgaccggaa
    14101 tgactttcat atcagtaata acaggactgg attgtgtcgt catcttattg ccccgcgaca
    14161 ggtttcagct cgatacgctt gatatcgccc accagcacca ggtagctcag taccgcgatt
    14221 aaggcatgaa caccaacata aatcagcgcc ccattaaagg agccagtcgt gccaacgata
    14281 taaccaattg cgattggcgt gacgatgcca gaaatgttac cgaacatgtt gaacaggcca
    14341 ccggaaagac cgctgatctc ttttggcgcg gtatctgcca ttactgccca acccagcgcc
    14401 ccgatgccct taccgaagaa ggccagcgcc ataaagccga tgatcatcca ctcaacgttg
    14461 acgtagttgc agaacaccat caccatcgac agcaacatgc ccattacgat cggtgtttta
    14521 cgcgcaatgt tcagcgatcc cgtgcggcgc atcagccagt cggaaataat cccacccagc
    14581 acaccgccga taaaaccgca aaccgccgga acggaagcca caaagcccgc tttcagaatt
    14641 gacatcccac gcgcctgcac cagataaacc ggaaaccagg taatgaaaaa gtaagtcagg
    14701 gcgttgatac agtactgacc gatataaacg ccgatcatca tccgtgaccc gagcagttgt
    14761 ttgatctgcc cccacttcac gctgaacggc actttaactt tggtgttttg ctgatccata
    14821 ttgatcagcg cgccacccgc ggcgatgtac tccagctctt tctgatttac ccccggatgt
    14881 tgatttggct cgtggatgac tttcaaccag ataaagctga tgacaatccc cagaccgccc
    14941 ataaagaaga agacgtgtga ccagcccact tcatgcgtca gccagcccat aatcggcgcg
    15001 aagatcaccg ttgcgaagta ttgagcggag ttaaaaatcg acaccgccgt tcccctttcc
    15061 tgcgccggaa accaagccgc aacaatgcga ctgttgccgg ggaaagatgg cgcttcagca
    15121 agcccgacca ggaagcgcag agtaaacagg gcaacgataa tgccgaatcc actaaagata
    15181 tcgacgaagc cttgcagcaa ggtaaacatc gaccagataa agatcgacca gaagtagacg
    15241 cgttttgaac caaaacgatc cagcaaccaa ccaccaggga tctgcccgat aacataagcc
    15301 catgagaagg cagagaacac atagcccatt cccacgggat caaggccgat atctttggcc
    15361 atttccgaac cggcaataga aagcgtagcg cggtcgccgt agttgaagga tgtgacgata
    15421 aacaacatca ccactatcca gtaacgagca ttcgtgcgtt tttccacact gctcgcagcc
    15481 tgacttaaag aactcgttgt tgcactcctg aaaattcgcg ttagccacgc taactctgga
    15541 ctgcgacata gccaggaaat cagaggtgac gtagggtgtt ttttgccgtt tttataggtc
    15601 gttcgccgaa tacggcgcgt gtttatatct ggcaatagca gtataaaaag cgcgccatag
    15661 cggctcaccg tgcaacaaca caacattaat gcgttcaatg aggcccgatt ttggcattag
    15721 ccctggacgg tggaatccac ttcacggaaa tgaaaacaag aacaagaaag gaagggttaa
    15781 aacgaagaaa taaagaagag tatgaaatgg atcgcttgac tccaggcaaa cgccagtaaa
    15841 aatccgcgct atgaagcagt ttttactggc atttgcctga aaagattcga ttcagcaccg
    15901 ctaaaacgac atttaccgct cgctgaacat atcaggacaa cagcgtgccc cactgttcga
    15961 cccacggatt tgattccgtt tccggttccg ggttctcact ggcgtcaatc aacagcattt
    16021 cgccaactcg ctgagcgctc tgttcctgca acaaggcatc gaactgtttg ccgccattgc
    16081 agaaatttac ataactgctg tcgccgagcg caatcacgcc ataacgcaga ttcggctgga
    16141 agcccagact atctttgatt ccctgaaaga gcggcacaat gctatcagga aggtcgccct
    16201 gcccggtcgt ggacgtaacc accagaacat acttatcctg ataaggcagc cagtcgctta
    16261 attcaggatc ttcaaatacc gttgctttgt ggccctgtgc ggtcagaatt gcttctgctt
    16321 cttccgccac taacagcgaa tttccgtaca tggtgccgac aaaaatacca atttccgcca
    16381 taccgttttc tccctggatt aggaacttat ctctccatcc tgacccgatg gcgcactgaa
    16441 ctcaaccctt tcattttcag gaagcagacc gcgccagcca aattgtgata acgcctgcat
    16501 ccaggtgtcg tccaggcccg cgtgaatagt cagcggctcg ccagtaaaag gatgcgtcaa
    16561 tgacaactga ctggcatgta gcattaaccg ctggaggcca aaatgctcag caccgctgcg
    16621 attctggcgt aaatcgccat gtttgctatc gccaataatc ggatgacgca aatgggcaag
    16681 atgtcggcga agctgatgtt tgcgtccggt tttcggctcc agttccacca ggccgtagcg
    16741 cgtggtcggg taacgtccgg tcgctaccgg catttctacg gtcgccagac cgcgataatg
    16801 cgtcactgct ggctgcgggc ctttatcttc gcgggcaaat ttatcagcga ttttgtccag
    16861 ttcttccacc agtggataat ccagcaccgc ttcttccatc aaccagccgc gcacaatcgc
    16921 atggtaacgt ttctggattt ggtgctgttc aaactgttgt gccagcagcc gtccggcctc
    16981 gctggataat cccatcaaca acacaccaga agtgggtcgg tccagacgat gagcagtaaa
    17041 aacatgctgt cctatctggt tacgcacggt ttgcatgacc actactttct cgtcgcgatc
    17101 cagccagcta cggtgaacca gccagccgga gggtttattt accgaaacca gccattcatc
    17161 ctgatagagt atttccagca ttagctcgca tcatccgcaa aaagagcatc cagtttttcc
    17221 aactcagcca gaataagcgc gtgttgcgga tggtccgtcg ccagcgccat ttcataatag
    17281 ggtgcaacag caaaagcgcc cggtaacggc tggttgttat ttaacaaatc gtgcattcgc
    17341 gggatcagca cccactgcaa ccactccagt ggttccatgg tgtccataaa gaacggttgg
    17401 gtactattaa attgatgcgg ctggggttca tcgtttcgcc agtgctgatg ttcacgcagt
    17461 aacgcttcaa gcgcctgcaa ctggagacga acgcggtcat gagtggtcat aaagcaacct
    17521 caataagaaa aactgccgcg aaggatagca ggaataaaaa aagggagcac tgtattcaca
    17581 gcgctcccgg ttcgtttcgc agcattccag ctacttttgt tgctccctgc tcatccttga
    17641 caacttttcc tctggccttg cggccaatcg ttcatcctga actattgctt cctgctcaca
    17701 ccaccccgat gtgatacttc atcctgaagt gtccttggcc ttcctgacca gccgaatcat
    17761 cctgaccggt tctcattctc catcctggag gtgtccttta acgcgtcctg cgtcatcctc
    17821 ttcgcttcat ccagaagcct ttccctgaaa caccatcctg gtgtgtcctg cagaagtgtc
    17881 atcatcctga tgttcacttc gttgtctgac tccctgtcga cgaagataga atcgtctttt
    17941 tcgcgaagtc ttacaaggcg cttacagaca atgccttaag gaaaatttca tatgaaagtt
    18001 aagaattaac aatcaacatt ttgattttaa atgaaaataa cgattcttat ctttttaagt
    18061 attcgcttat ttcctggcga tctcctacag agtctgtaag agatctctca caaccagatg
    18121 gggtatttgg attacagaag aggcttaagt tgattaagga attccgcaag atttgaggct
    18181 aaatgcgtcc gtttacgtgt tcccagcgtc tctttgcaca cttcaccgga gagattacac
    18241 accgagatga cctctaactc ttcttccagc gtcgcgataa atagcgtggg cggcagtttt
    18301 agccttttct gagtcaccag atgaccaatc agattttcct gcacccgacg gaagtcgtct
    18361 tcactccagg tttgcagcaa tgtcagtttg atatcgccaa actgcgcgtg catatccccg
    18421 gcaaactgag tcgtataaaa ggtatgaatt gtgggttgta tcacaatatc aaaagcgcgt
    18481 tcaaccgcgt ttacattttg ttccccggta aacggctggg gttgccagta tacggcatct
    18541 tcggtagttg aaataatgca cggtgaagga acaccgtaca gttcctcact taacggccag
    18601 cttttatgct cttcatgcca cgcatcgcag tagcgtgcag taaaatcttt cagggcttgt
    18661 gcggtcaaat cgtccactaa tttctctctt cacttaaacc agatacactt gtcgtttagt
    18721 ttatctggtt tatgacggtg aaacatgtct tcttatgcaa accatcaggc acttgcgggc
    18781 ctgactcttg gaaaatcaac cgattaccgg gatacctatg acgccagcct actgcaaggc
    18841 gttccacgca gcctgaatcg cgacccgctg ggtctgaaag cggataacct gccttttcac
    18901 ggtacggata tctggacgct gtatgaactt tcctggctga atgcgaaagg tttgccgcag
    18961 gtcgctgtcg gtcatgttga acttgattac accagcgtaa atctgattga gtcgaagagt
    19021 tttaagctct atctcaacag ttttaaccag acgcgtttta ataactggga tgaggtgcgc
    19081 cagacgctgg agcgcgactt aagcacttgc gctcagggta agattagcgt ggcgttatat
    19141 cgtcttgatg aactggaagg ccagccgata ggtcatttta atggcacttg cattgatgac
    19201 caggatatca ctatcgataa ctatgaattc actactgact atctggagaa tgccacctgt
    19261 ggtgaaaaag tagtggaaga gacgcttgtc agccacctgc tgaaatcaaa ctgcctgatc
    19321 acccatcaac cagattgggg ttcgatccaa attcagtatc gtggacgcca aattgacaga
    19381 gaaaaactgc tgcgttacct ggtctcattc cgtcatcaca acgagttcca cgaacagtgc
    19441 gtggaacgca tctttaatga cctgttacgc ttctgccagc cagaaaaatt gagcgtttac
    19501 gcacgttata cccgtcgtgg cggtctggac attaacccgt ggcgcagtaa tagcgatttt
    19561 gtcccatcga ccacaagact ggttcggcaa taaatttttt ctcaattttg cgtgctggat
    19621 tcacgcagaa ggttgtgaaa ggtcatcagg cagggctatt gtaatcaaag ggaatgacga
    19681 tattcgtccc ataaggagtt tttcttgatt acacatatta gcccgcttgg ctccatggat
    19741 atgttgtcgc agctggaagt ggatatgctt aaacgcaccg ccagcagcga cctctatcaa
    19801 ctgtttcgca actgttcact tgccgtactg aactccggta gtttgaccga taacagcaaa
    19861 gaattgctgt ctcgttttga aaatttcgat attaacgtct tgcgccgtga acgcggcgta
    19921 aagctggaac tgattaatcc cccggaagag gcttttgtcg atgggcgaat tattcgcgct
    19981 ttgcaggcca acttgttcgc ggtcctgcgt gacattctct tcgtttacgg gcaaatccat
    20041 aacaccgttc gttttcccaa cctgaatctc gacaactccg tccacatcac taacctggtc
    20101 ttttccatct tgcgtaacgc tcgcgcgctg catgtgggtg aagcgccaaa tatggtggtc
    20161 tgctggggcg gtcactcaat taacgaaaac gagtatttgt atgcccgtcg cgtcggaaac
    20221 cagctgggcc tgcgtgagct gaatatctgc accggctgtg gtccgggagc gatggaagcg
    20281 ccgatgaaag gtgctgcggt cggacacgcg cagcagcgtt acaaagacag tcgttttatt
    20341 ggtatgacag agccgtcgat tatcgccgct gaaccgccta acccgctggt caacgaattg
    20401 atcatcatgc cagatatcga aaaacgtctg gaagcgtttg tccgtatcgc tcacggtatc
    20461 attatcttcc ctggcggtgt gggtacggca gaagagttgc tgtatttgct gggaatttta
    20521 atgaacccgg ccaacaaaga tcaggtttta ccattgatcc tcaccggccc gaaagagagc
    20581 gccgactact tccgcgtact ggacgagttt gtcgtgcata cgctgggtga aaacgcgcgc
    20641 cgccattacc gcatcatcat tgatgacgcc gctgaagtcg ctcgtcagat gaaaaaatcg
    20701 atgccgctgg tgaaagaaaa tcgccgcgac acaggcgatg cctacagctt taactggtca
    20761 atgcgcattg cgccagattt gcaaatgccg tttgagccgt ctcacgagaa tatggctaat
    20821 ctgaagcttt acccggatca acccgttgaa gtgctagctg ccgacctgcg ccgtgcgttc
    20881 tccggtattg tggcgggtaa cgtaaaagaa gtcggtattc gcgccattga agagtttggt
    20941 ccttacaaaa tcaacggcga taaagagatt atgcgtcgta tggacgacct gctacagggt
    21001 tttgttgccc agcatcgtat gaagttgcca ggctcagcct acatcccttg ctacgaaatc
    21061 tgcacgtaat ctcccctccc ccggtgacgt ttgttgccgg gttttctcgt ttttggtcac
    21121 ttactcatca actcatttca tttgttatat gaatgtttct taccaccctc acgcgacaaa
    21181 tatcatcaca gttaatatgt catacaattt atgttgcaac gcaaacgttt ccctattttc
    21241 ataaaccgta ttttatcgct atgaaaaaga aattatcgcc atgattaact aaaagtattg
    21301 attttttcag ttcaacctac atatattgcg cgccctggaa gaagtcagat gtcgtttaat
    21361 gggcaaatat tgcccttaaa ttctctttta cttttgattt acagagtaaa gcgttgggat
    21421 aatctatctt ccaagtagat tattgtattt gagatcaaga tcactgatag atacataact
    21481 tgtgtgtatc tttccgccct caaattatta cggcggtaaa tgattaagcc atcgccgata
    21541 gacagatttc atttttacgg tcaggcacct tcccgggctg aactggctaa aagctgaatt
    21601 atttgcattc ctccaggaga aatagatgga aacgactcaa accagcacga ttgcgtcgaa
    21661 agactctcgt agtgcctggc gcaagacaga caccatgtgg atgctgggcc tttacggcac
    21721 ggcaatcggc gcgggcgtgc tgttcctgcc aatcaacgcc ggtgttggcg gtatgatccc
    21781 gctgatcatc atggctatcc ttgcgttccc gatgacgttt tttgctcacc gcggcctgac
    21841 tcgcttcgta ctgtctggta aaaacccggg cgaagacatc accgaggttg tagaagaaca
    21901 ctttggtatt ggcgcaggta aactgattac cctgctctac ttcttcgcta tctacccgat
    21961 cctgctggtt tatagcgtgg caatcaccaa tacagttgaa agcttcatgt ctcaccagct
    22021 gggtatgacg ccaccgccgc gtgcgattct gtcgctgatc ctgatcgtgg gtatgatgac
    22081 catcgttcgc ttcggtgagc agatgatcgt taaagcgatg agtattctgg tgttcccgtt
    22141 tgttggcgta ttgatgctgc tggctctgta cctgatcccg cagtggaacg gcgcagcact
    22201 ggaaacgctg tctctggaca ctgcatctgc aaccggaaac ggtctgtgga tgaccctgtg
    22261 gctggcaatt ccggtaatgg tgttctcgtt caaccactct ccgatcatct cttctttcgc
    22321 cgttgcgaag cgtgaagagt acggcgatat ggcagaacag aaatgctcga agatcctggc
    22381 attcgcacac atcatgatgg tgctgaccgt aatgttcttc gtcttcagct gtgtactgag
    22441 cctgactccg gcagacctgg ctgcggctaa agagcagaac atctcgattc tgtcttacct
    22501 ggctaaccac tttaacgcac cggttatcgc gtggatggct ccgattatcg cgattatcgc
    22561 tatcaccaaa tccttcctcg gccactacct gggcgcacgt gaaggcttca acggtatggt
    22621 gattaaatct ctgcgtggta aaggtaagtc tatcgaaatc aacaagctga accgtatcac
    22681 tgcgctgttc atgctggtaa cgacctggat tgttgccacc ctgaacccga gcatcctggg
    22741 tatgattgaa accctgggcg gtccaatcat cgcgatgatc ctgttcctga tgccgatgta
    22801 cgcaattcag aaagtaccgg caatgcgtaa gtacagcggt cacatcagca acgtattcgt
    22861 tgtcgtgatg ggtctgattg caatctccgc aatcttctac tctctgttca gctaagtcct
    22921 ttcgcgccgc tttcgggcgg cgcttcctcc gttttaacgc gatgtatttc ctatgattag
    22981 cgtattcgat attttcaaaa tcggcattgg cccttccagt tctcataccg ttggaccaat
    23041 gaaagcgggt aaacaattta ccgacgatct gattgcccgt aacctgctta aagacgtgac
    23101 ccgcgtggtg gttgacgtgt acggctcgct ctctctgacc ggtaaaggcc accacactga
    23161 tatcgccatt attatgggcc tggcgggtaa cctgccggat accgtggata tcgattccat
    23221 ccctggtttt attcaggatg tgaatactca tggtcgcctg atgctggcaa acggtcagca
    23281 tgaagtggag ttcccggttg atcagtgcat gaacttccac gccgacaacc tttctctgca
    23341 tgaaaacggt atgcgcatta ccgcgctggc gggcgataaa gtcgtttaca gccagactta
    23401 ctactctatt ggcggtggct ttatcgttga tgaagagcat tttggccagc aggatagcgc
    23461 accggttgaa gttccttatc cgtacagttc agcagccgat ctgcaaaaac attgtcagga
    23521 aaccgggctg tcactctctg gcctgatgat gaaaaacgag ctagcgctgc acagcaaaga
    23581 agagctggaa cagcacctgg cgaacgtatg ggaagtcatg cgtggcggta ttgagcgcgg
    23641 tatttccacc gaaggcgtgt tgcctggcaa actgcgcgtt ccacgccgtg ctgcggcact
    23701 acgccggatg ctggtcagcc aggataaaac caccactgac ccgatggcgg ttgttgactg
    23761 gatcaacatg tttgcactgg cagtgaacga agagaacgct gctggcggac gcgtggtgac
    23821 tgcgccgact aacggtgcgt gcgggattat cccggcagtg ctggcgtact acgacaagtt
    23881 tatccgcgaa gtgaacgcta actcactggc tcgttacctg ctggtagcca gtgccattgg
    23941 ttctctttat aagatgaacg cgtcaatttc tggtgctgaa gtcggctgcc agggtgaagt
    24001 tggtgtggcg tgctcaatgg cggcggctgg tctggcagaa ctgttaggtg caagcccggc
    24061 gcaggtgtgc atcgcggcgg aaatcgccat ggagcacaac ctcggtctga cgtgtgaccc
    24121 ggtcgccgga caggtccagg tgccatgcat cgagcgtaac gccattgcgg cagtaaaagc
    24181 ggtgaacgcc gcacgtatgg cgctacgccg taccagcgag ccgcgcgtct gcctcgataa
    24241 agttatcgaa accatgtacg aaacaggtaa agatatgaac gccaagtacc gcgaaacctc
    24301 tcgcggcggc ctggcaatga agatcgttgc ctgcgattaa tcgctctcca gaggcctcgt
    24361 tttgcgaggc ctcttcccga tttctcatcc agccgtagcc tgttccggca tcgaatgtta
    24421 cccttatcgc ctgatcttta agggggttat cgtggctgtt catttgctta ttgtcgatgc
    24481 actgaatctt attcgtcgca ttcatgccgt tcagggatcg ccctgtgtcg aaacctgcca
    24541 gcatgcgctc gatcagctca ttatgcacag ccagccaacc cacgcggtcg ccgtttttga
    24601 tgatgaaaac cgcagtagcg gctggcgtca tcagcgttta ccagattaca aagcgggtcg
    24661 accgccaatg ccggaagagt tgcacgacga gatgcctgca ttacgcgccg cctttgagca
    24721 acgcggcgtc ccgtgctggt caaccagcgg caacgaagcc gatgacttag ccgccacgct
    24781 ggcggtcaaa gtgacacagg ccgggcatca ggcaacgatt gtttcgacag ataaaggcta
    24841 ctgtcagtta ctttcaccga cattacgtat tcgtgattac ttccagaaac gttggctgga
    24901 tgcgccattt atcgataaag aatttggcgt tcaaccgcag cagttgcccg attactgggg
    24961 acttgcgggg atcagcagtt caaaggtacc gggtgttgcg ggaatcggac caaaaagcgc
    25021 cacgcagctg ctggtcgagt ttcagagtct ggaagggata tatgagaatc tggatgcggt
    25081 tgccgaaaag tggcgcaaaa aattagaaac ccataaagag atggcgtttc tgtgccgcga
    25141 tattgcccgc ttacaaaccg atttgcatat cgacggcaat ttacagcaat tgcggttggt
    25201 acggtaacgg cgagccggat gcaaacgatt atcaacacat tgaatttaat gcacttatat
    25261 accggataag acgcggctag cgtcgtatcc ggcattatca catcagcgca tttaccaggc
    25321 ggtatggtaa agctctacaa tatcctcaag cgttgcttca cgcgggttgc caccggtaca
    25381 aacatcatcc agtgccgcct gcgccagtgc cggaatgtct tccttgcgta caccaacatc
    25441 acgcaaatgt ggcggaatac cgacatcacg gttgagagca aacaccgctt caacagcggc
    25501 attacgcgcc tcttccagcc actttcacgc ccataacgcg cgcgatatcg cggtacttct
    25561 caccggtaaa gtcagcgtta tagcgcatga catgcggtaa caggatggcg ttcgcaacac
    25621 cgtgtggagt gttgtaaaac gcgcccagtg gatgcgccat accatgcacc aaccctaacc
    25681 caacattcga gaagcccata cccgcaacat actgcccgag cgccatttct tctccggcat
    25741 ccttatcacc agcaaccgat cctcgcagcg ccccagcaat gatttcaatc gctttaatgt
    25801 gcagtgcatc ggttagcgcc cacgcgccac gggtaatata cccctcaata gcatgagtga
    25861 gcgcatcgac acccgtcgca gctttcagcg ctggaggcat accatccatc atgtcagcgt
    25921 caataaacgc cacctgcggg atatcatgcg gatcaacgca aacaaacttg cgccgtttct
    25981 cttcgtcagt gatcacgtag ttaatggtca cttctgccgc agtacctgct gtggtaggaa
    26041 ttgccagaat cggtacactg ggtttattgg tcggggaaag cccttccagg ctacgcacat
    26101 cggcaaactc cgggttgttg ctgataatgc caatcgcttt acaagtatcc tgtggagaac
    26161 caccaccaat agcgatcagg taatccgcgc cgctattctg gaatacaccg agcccttctt
    26221 tgacgacagt aattgttggg ttgggcacta cgccgtcgta aatcgcccat gccagccctg
    26281 cagcatccat cttatcggtc actttcgcca ccacgccgca ttgcaccagc gttttatcgg
    26341 tgacgatcag cgccttctga taaccacggc gtttcacctc atcggttaaa gccccaacag
    26401 caccccgacc aaaccatgcc gtttcgttca gaatcattct gttagccatc atccttctcc
    26461 ttgttgcttt acgaaattac tcttcaatcc gtaacccata ggttttgaat ttctccagca
    26521 ctacggcaat ctcttcatcg ctcagcactg gcaccgggtc cgtaatcgcc agggtcgtca
    26581 ggtaaagttg cgccagcact tcaacttcat gcgccagcca taacgctttt tccagattca
    26641 cctcacaagc gataagccca tgatgttgta acaaagttgc cttacgattt ttgagagcca
    26701 gcgcaacatg ttcagaaagt tcgcgtgttc caaaggtcgc ataaggcgcg caaggaatag
    26761 aattaccacc tgccgccgca atcatgtagt gaatagcggg gatcggtcgg ttaagaatgg
    26821 aaactgctgt gcaatgaacg gcatgattgt gaacaaccgc gttggcatcc ggtctgcttt
    26881 gataggccgc catatggaaa cgccattcgc ttgaggggag ctttccttcc tcatgtttac
    26941 cgttaccatc aataaaggca atatgcgact ccgtcagttt ttcatatgga atgcctgtag
    27001 gcgtaatcag catcccatcc tgataacgta cactgacgtt ccccgctgtc ccctggttca
    27061 gtcccaggcg ggtcatttcc aggcaagtgt caataatctg acgagcaagt ttatttcgtt
    27121 ccattagcta cctctctctg attcaaaaca gggcaataat gttgttcctt tcacactatt
    27181 gaattagccg tttaattacc caccat
//
LOCUS       DAJVUJ010000042        26579 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.042, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000042 DAJVUJ010000000
VERSION     DAJVUJ010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26579
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.042"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(<1..316)
                     /gene="rpnB"
                     /locus_tag="OQQ48_001615"
     CDS             complement(<1..316)
                     /gene="rpnB"
                     /locus_tag="OQQ48_001615"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HCS1952425.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQN"
     gene            complement(513..1286)
                     /gene="hisP"
                     /locus_tag="OQQ48_001616"
     CDS             complement(513..1286)
                     /gene="hisP"
                     /locus_tag="OQQ48_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HCS1952426.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEAHERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(1294..2010)
                     /locus_tag="OQQ48_001617"
     CDS             complement(1294..2010)
                     /locus_tag="OQQ48_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1952427.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGIYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVLHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(2007..2693)
                     /gene="hisQ"
                     /locus_tag="OQQ48_001618"
     CDS             complement(2007..2693)
                     /gene="hisQ"
                     /locus_tag="OQQ48_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HCS1952428.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFFGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(2783..3565)
                     /gene="hisJ"
                     /locus_tag="OQQ48_001619"
     CDS             complement(2783..3565)
                     /gene="hisJ"
                     /locus_tag="OQQ48_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HCS1952429.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(3786..4568)
                     /gene="argT"
                     /locus_tag="OQQ48_001620"
     CDS             complement(3786..4568)
                     /gene="argT"
                     /locus_tag="OQQ48_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HCS1952430.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(4834..5403)
                     /gene="ubiX"
                     /locus_tag="OQQ48_001621"
     CDS             complement(4834..5403)
                     /gene="ubiX"
                     /locus_tag="OQQ48_001621"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HCS1952431.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(5498..7015)
                     /gene="purF"
                     /locus_tag="OQQ48_001622"
     CDS             complement(5498..7015)
                     /gene="purF"
                     /locus_tag="OQQ48_001622"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCS1952432.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQDYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(7052..7540)
                     /gene="cvpA"
                     /locus_tag="OQQ48_001623"
     CDS             complement(7052..7540)
                     /gene="cvpA"
                     /locus_tag="OQQ48_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HCS1952433.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(7799..8461)
                     /gene="dedD"
                     /locus_tag="OQQ48_001624"
     CDS             complement(7799..8461)
                     /gene="dedD"
                     /locus_tag="OQQ48_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HCS1952434.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(8451..9719)
                     /gene="folC"
                     /locus_tag="OQQ48_001625"
     CDS             complement(8451..9719)
                     /gene="folC"
                     /locus_tag="OQQ48_001625"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCS1952435.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSAKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(9789..10703)
                     /gene="accD"
                     /locus_tag="OQQ48_001626"
     CDS             complement(9789..10703)
                     /gene="accD"
                     /locus_tag="OQQ48_001626"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HCS1952436.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVEMGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPLGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(10859..11518)
                     /locus_tag="OQQ48_001627"
     CDS             complement(10859..11518)
                     /locus_tag="OQQ48_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCS1952437.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRCSYLDKTHQFYERHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLIFTYAGYFFGMLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            complement(11601..12413)
                     /gene="truA"
                     /locus_tag="OQQ48_001628"
     CDS             complement(11601..12413)
                     /gene="truA"
                     /locus_tag="OQQ48_001628"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCS1952438.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAPRKDAAWTLGVNANLPDDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(12413..13426)
                     /locus_tag="OQQ48_001629"
     CDS             complement(12413..13426)
                     /locus_tag="OQQ48_001629"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCS1952439.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(13492..14628)
                     /gene="pdxB"
                     /locus_tag="OQQ48_001630"
     CDS             complement(13492..14628)
                     /gene="pdxB"
                     /locus_tag="OQQ48_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HCS1952440.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIAVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            14727..15722
                     /gene="flk"
                     /locus_tag="OQQ48_001631"
     CDS             14727..15722
                     /gene="flk"
                     /locus_tag="OQQ48_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HCS1952441.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(15719..16897)
                     /locus_tag="OQQ48_001632"
     CDS             complement(15719..16897)
                     /locus_tag="OQQ48_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1952442.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPLKFALLVVGRLILGFGESQLLTGALTWGLGIVGAKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(17226..18446)
                     /gene="fabB"
                     /locus_tag="OQQ48_001633"
     CDS             complement(17226..18446)
                     /gene="fabB"
                     /locus_tag="OQQ48_001633"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HCS1952443.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMKMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            18605..20611
                     /gene="mnmC"
                     /locus_tag="OQQ48_001634"
     CDS             18605..20611
                     /gene="mnmC"
                     /locus_tag="OQQ48_001634"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HCS1952444.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQQDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQDAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSAPWFNRTGSSKREAAIIGGGIAC
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(20732..21010)
                     /locus_tag="OQQ48_001635"
     CDS             complement(20732..21010)
                     /locus_tag="OQQ48_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HCS1952445.1"
                     /translation="MIAEFESRILALIDGMIDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(21044..21592)
                     /gene="epmC"
                     /locus_tag="OQQ48_001636"
     CDS             complement(21044..21592)
                     /gene="epmC"
                     /locus_tag="OQQ48_001636"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HCS1952446.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(21592..22401)
                     /locus_tag="OQQ48_001637"
     CDS             complement(21592..22401)
                     /locus_tag="OQQ48_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCS1952447.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFAGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(22401..23225)
                     /gene="mepA"
                     /locus_tag="OQQ48_001638"
     CDS             complement(22401..23225)
                     /gene="mepA"
                     /locus_tag="OQQ48_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HCS1952448.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFAPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(23229..24314)
                     /gene="aroC"
                     /locus_tag="OQQ48_001639"
     CDS             complement(23229..24314)
                     /gene="aroC"
                     /locus_tag="OQQ48_001639"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCS1952449.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGILLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(24349..25281)
                     /gene="prmB"
                     /locus_tag="OQQ48_001640"
     CDS             complement(24349..25281)
                     /gene="prmB"
                     /locus_tag="OQQ48_001640"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCS1952450.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPDAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREYFAIYKD"
     gene            25447..25998
                     /gene="smrB"
                     /locus_tag="OQQ48_001641"
     CDS             25447..25998
                     /gene="smrB"
                     /locus_tag="OQQ48_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HCS1952451.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(26071..26574)
                     /locus_tag="OQQ48_001642"
     CDS             complement(26071..26574)
                     /locus_tag="OQQ48_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HCS1952452.1"
                     /translation="MFVYYDGNLHGCPWIVSSFVKSRDPFAKTYDDDFPDYIGPTKVS
                     SSCPAVPLAPYDVSWNENYVVHNKVVRLQSTGGVIEQTLPTFLMENGKLCNGNNFDER
                     GVYCRFIAQQMTFSTSGCDNAKVTVTPEPQPITSRQLHDMKLRVDTTSRQPIDSTCRF
                     TYILNMY"
BASE COUNT         6540 a         7283 c         6715 g         6041 t
ORIGIN      
        1 ggttttgcat tgccgcaatg gaataacgca tcatgcgaaa agccattaat tcttccggct
       61 tactttggtg ctctatcact acataaatat aacccactcc ctcctgcgtt ttcacagacc
      121 acaagaggtc ggaataatat tgccgcaggt cttcatcaat aaaactgttt ggttccagtt
      181 taagcgtcgt taaatcacac agtttgcgca gcggcgcggg aagatgaata tcaataaaat
      241 cccgcgcggt gtctggatgg cgtaaaaaag atttaaatac cgcatcatgc ggcgtggaag
      301 ttgtcgatat tgtcatggcg ttccgtcacc cctcaaattt aagatgacgc gacaataacc
      361 acgattaaca cccaatgcag caggcagttt ttatctttgg acagcgaatt cagaagtatt
      421 tttagcggac tgcaactttt acatttcagc ggaagctgac tcgcaaattc cccatgaaaa
      481 tactccggca gcgttagcca ccggagagga aattatttca gcgatccctt aaggaactgt
      541 tgcagacgag ggctttgcgg gttgccaaat aactgctccg gcgcgccctc ttcttctatt
      601 ttcccctgat ggaggaaaat gacatgagta gaaacatggc gagcaaagcc catttcgtga
      661 gtcactacca ccatggtttt cccctcttct gccagttgct gcataatacg caacacttcg
      721 cctaccagtt caggatcgag cgccgaggta ggttcatcaa acagtaaaac ttccggttcc
      781 atcgccagcg cccgcgcgat agaaacacgc tgttgctgac cgccggaaag atgcaccgga
      841 tatttcccct gcgcacgttc gtctatcccg acttttgcca gatacttgac cgcccgctcg
      901 tgcgcttcct gcttgctcag gcccaacacc tgaatcggcg cttccatgac gttttccagc
      961 accgtcatat ggctccagag attgaagtgc tggaatacca tcgtcaggcg tgtgcgcagt
     1021 aagcgcagtt gatttttatc ggcgactttg agttgaccgt ctttgtcgcg caccagattg
     1081 atcgtctggc cattgaccac gatcgaccct tcactcggtt tttcgaggaa gttaatgcag
     1141 cgcagaaagg tacttttccc cgatcccgac gatccgatga tgcttattac atctccggca
     1201 ttcgcttgca gtgagacccc tttcagcact tcatgttcgc cgtagcgttt gtgcaaatcg
     1261 ataacgttta atttattctc ggacatcgta ttctcagtgc gttgaagaag gtttcacatg
     1321 ctgcaaccag cgtttttccg ctctgcgaaa gaggctgatc aggacataag agatgattaa
     1381 atagagcacc gcggcaatgc cgaaggcggt aaaaggttga tacgtggcgg cgttaatatc
     1441 gcgggctatt ttcagcagat ccggcacagt ggccgtaaac gccagcgcag tagagtgcag
     1501 catcaggatc acttcgttgc tgtatgccgg taacgcaata cgcagcgcag aaggcaaaat
     1561 aatgcaacga tacattttaa aagtcgagaa gccataggct ctggcggctt caatttcccc
     1621 atgcagaacc gaacggattg ccccagcaaa aatctcggta gtgtaagcgc aggtgttaag
     1681 cgtcagcgcc agcacggtac agttcaggcc actgcggaag aaagcgttaa ggaattcggt
     1741 tcccttaaca atctcaagcg tgtatatgcc ggaatagaac accagcaact gaacatacag
     1801 cggcgtaccg cgaaaaatat aggtaaataa ccagattgga aactggatgt atttattact
     1861 ggagacacga ccaatcgcca gaaacagcgc caggactccg cctatcacta ccgacaaaat
     1921 aagcagccac agagtgatcg ccacaccagt aaagcgataa ccgtcggtcc acagcagcgg
     1981 tttccagtat tcatgtaaga tttcgatcac aggtcagccc tcttcacacc cacggagtag
     2041 cggcgctcaa ggaacagcag cacaccattg gaaacagtgg tgaaaaccag gtaaatcacg
     2101 ccacagacga tggcgaaata gaacggttcc caggtacttt tgcctgccag ttgggtggct
     2161 ttgaccacat cttccaggcc gagtaacgaa accagtgcgg tagatttgag gatcacctgc
     2221 cagttgttgc caatgcctgg cagggcgtaa cgcatcatcg ccggaaacat gatccgccga
     2281 aacacttgcc cacgagtaaa accgaacgcc gtcgccgcct ctatatgtcc tttcggcact
     2341 gccataaaag caccacggaa cgtttcggta aaataagcac cgtagataaa accgagagtg
     2401 ataataccag cgaccatcgg atcgatatca atctgcccga cgcccatcgc ctccgtcacc
     2461 gtgtttagcg caatctgcaa accgaagaaa atcagcagca tcaacactaa gtccggcacg
     2521 ccacgaatca gcgtggtgta cccttcgaaa atcagccccg aaagccgatt ttgcgagagc
     2581 ttaccgccag cgccaattaa accgatgatt acagcgagca ctacagagct gatagccagc
     2641 tccagcgtga cgagcgcccc ctgtaaaata acacctgaaa acccatataa catgctgcct
     2701 gtcctgtcgt gtgtggtgaa ttaccgcctc ttgtctccct ccggttaccc gaagggagcg
     2761 ggctttcttt caccgatggc gattagccac cataaacatc aaaatcgaag tactttttcg
     2821 ctaatttctc gtaagtaccg tcagcgcgca tttcggcaaa ggctttgttc agtgcttcgc
     2881 gcagttcgtt atcttcttta cgcaggccca tgccggtccc tacgccaaac agtttttcat
     2941 ctttaacaga cgggccaccg aatttgtaat ctttaccgac aggttgtttg aggaaacctt
     3001 cgctggcagc gacctcatcc tggaacgcgg catcaatacg tccggcagtc aggtcagaat
     3061 aaatgttgtc ctgcccctga tacgagacga tttcaatgcc ttttggtgcc caatgttcat
     3121 taccgaacgt ctcctgggtg gtgccctgca atacgcctac ccgtttgcct ttcagcgact
     3181 cgactgtcgg ctgaatgtca gaatttttcg ccaccaccaa acgagaatcg gcagcgtaca
     3241 gtttgtcggt gaaggctatt tcttgctgac gtttttccgt aatggaaagc gatgacatga
     3301 tggcgtcaat cttcttcgct tttaaggacg ggattaacgc atccagcgga ttttcgacaa
     3361 acgtacattg cgtattgatg cgtttgcata attcctttgc cagatcgata tcgaagccaa
     3421 ccagttcgcc ttgtgaattt tttgattcaa atggcgcata ggtcgggtcg gtaccgatgc
     3481 ggatgttttg cggaatcgca gcaaatgccg cagttgcgct ggagaaggcc agaaccagag
     3541 agagcgatag caccagtttt ttcataatgt cctcaactga cagtcttttt atagggaata
     3601 tttacaggtt tgttacactt atcgtgccat aaaattgacc atcaaggcaa aatatcccgc
     3661 cccgttagat gtattatttt tcattaaaaa tgcttaagta tgcatttcaa tgcaccacga
     3721 tagtgcaaca aacctagcgt gcaccatgat gatcattcca tcaggtacag cttcccagcg
     3781 acgtatcagt caccgtagac attaaagtcg aaatactttt tcgccatctt gtcgtaggtg
     3841 ccgtcctgac gcagctcgcc aagcgcctta ttgaaggcag ccgtcagttc agcatcatct
     3901 ttacgtagcc ctacaccggt gccatcaccg aagtattttt tgtcttttac tgatgagcca
     3961 gcaaaggcga aatctttacc agcaggttgc ttgaggaatc cttcgctggc agcaacttca
     4021 tcttgtaacg cagcatccag acgtcctgca gccagatcgg aatagaccaa atcctggttg
     4081 gcataggcca ccacatccac gcctttgcta cgccaggtct cgttagcgta agcttcctgg
     4141 gttgatccct gcagcacacc aacatgttta cctttcagtg aatccagcgt tggctgaatc
     4201 ggtgaacctt tggcagcaat caaacgagaa tctgcggcgt acagcttgtc ggagaaggca
     4261 atctcctgct gacgtttatc ggtaatggaa agcgacgaaa taatggcgtc gatttttttc
     4321 gctttcagtg aggggatcag cgcgtcaaag tcactggcaa cccaggtaca tttcacctgc
     4381 atccgtttgc acatctcgtt accgagatcg atatcaaagc caacaaaatc acctttagca
     4441 tctttcgacg agaacggcgc ataggtggta tcggttccga tacgtaccgt ctccggtagc
     4501 gccgcatagc tggaagccgc tgcggagaga ccgactaaca aagacagagc gagaatcgac
     4561 ttcttcatac ataaccctca agtgaaaatg gcttttttat gttgtgtatt gtgttgtgtg
     4621 tttgcaggct ctttcatgca ggtcttatgc catcttgacg acaacagtaa cattcaacgt
     4681 taaatatgtt aataagacgt tgcattattg tcctgaagtt gaagatagca ggtatagcgg
     4741 ttgaatcgca gcgtttcgat tgaatggcag aaacaaaatg tcgaggattt gatcgcggtt
     4801 gcaaaattgc cctgaaacag ggcaacagcg gagttatgcg ccctgccagc gggcaaagag
     4861 atcttcagga agggttattg caaactggtc aagaacacga ttaaccgtct gatttatcac
     4921 atcatcaagg gattgcgggc gatgataaaa cgccggaaca ggaggcataa tcaccgcacc
     4981 gatttctgcc gcctgagtca ttaaacgcag atggcctaag tgcaatggtg tttcacgcac
     5041 gcagagcacc aacgggcgac gctctttcag caccacatct gccgcacggg tcagtaagcc
     5101 atcagtatag ctatggacaa tgccggaaag ggttttgatt gaacagggta aaatcaccat
     5161 ccccagcgtc tggaaagaac cggaagagat gctggcggaa atatcgcgcg catcgtgcgt
     5221 gacatcagct aatgcctgca cttcgcgcag ggaaaaatcc gtttcgaggg ataaggtctg
     5281 gcgcgccgcc tggctcatca ccagatgcgt ttcgatatct gtgacatcgc gcagaacctg
     5341 taataagcgc acgccataaa tcgcgccgct ggcaccgctg atgcctacaa tgagtcgttt
     5401 cataaatagt tgccctgtca gacttgcagg cagactttgc agtatttcgg cgggagttgc
     5461 aagtcagggt gccagaccgg caccctcagc gaaggcatca tccttcgtta tgcatttcga
     5521 gattttccac ttcgttctga cgttgcactg ctttggcgtc atcattacgt aacgtatcga
     5581 ggaaatcgag gtagtcctga tcaacatctt tggtgacgta gacgccgttg aacaccgagc
     5641 attcaaactg ctggatatcc ggattttcag cgcgaacggc gtcgatcaga tcgttcagat
     5701 cctggaaaat caacccgtca gcaccgatga tctggcgaat ttcatcaact tcgcgaccgt
     5761 gagcgatcag ttccgtggcg ctcggcatat caataccata aacgttcggg aagcgaattt
     5821 ccggggccgc agaagcgagg tacactttct tcgctccggc ttcgcgtgcc atctcgataa
     5881 tctgctcaga agtggtgcca cggacgatgg agtcgtcgac cagcaggacg tttttatcgc
     5941 ggaactcggc gcggttggcg ttcagtttac ggcgcacgga cttacgacgc agctgctggc
     6001 ccggcatgat gaaggtgcgg ccaacatagc ggtttttaac gaagccctgg cggtacggct
     6061 tgcccagaat acgagcaatt tccagcgcga tatcacacga ggtttccggg atcgggatca
     6121 ccacgtcgat atccagatct tcccattcgc gggcaatttt ctcacccagt ttcgtgccca
     6181 tattcacacg cgcgctgtaa acggaaattt tgtcgataaa cgagtccggg cgggcaaagt
     6241 atacatactc aaacaggcac ggattgctga ccggattgtc agcacattga cgggtaaaca
     6301 actgcccttc ttcagtgatg taaatcgctt cgcccggcgc gacgtcacgc aggaaatcaa
     6361 agcccagcgt atcgagcgct acgctttcgg aagcgaccat atattctgta cggttctcgt
     6421 caatatcacg ttttcccagt accagcggac gaatcccgtt tggatcgcgg aaagcaacca
     6481 taccgtggcc gataatcatc gccacacagg catatgcgcc gcggattaag cggtttgtgg
     6541 cagcaatggc agcgaaaata ttgtcggctt ccagcgggta gtggcggaag ttgtccagct
     6601 cgctagcgaa gatattaagc agaatttccg agtcggaagt ggtgttgatg tggcggcgtt
     6661 tttcttcaaa cagtttttta cgcaactcgt gagcgttggt cagattgccg ttgtgggcaa
     6721 gcgtaatgcc atacggggag ttaacgtaaa acggctgcgc ttcagaggcg ctggagctgc
     6781 cagccgtagg gtaacgcaca tgaccaatgc ccatattgcc ctgcaaacgc tgcatatggc
     6841 gagcttcaaa tacatcgctc accagcccgt tcgctttacg caaacggaag cagttattgg
     6901 catctatggt gatgatgccg gcggcatcct gaccgcgatg ctgaagcacc gttaaggcat
     6961 cataaatcga ctggttaacc ggcataacac cggcgatacc gacaataccg cacatacgtc
     7021 tttttcctcg ttaagccaca tctcagagca cttacgctct gggcaagaaa cttgacgagc
     7081 tttgcagata atcaaaaaac catctgatga taaaactgaa ctgcgggatc agctgtgatt
     7141 tgctccagtc ttcgcttttc gacaccccgg taaaggagtc gagaaagaag agaatggcgg
     7201 caacaatcaa cacaccgcgc aacgcaccga aacagacgcc cagcacccga tcggtgcctg
     7261 acaaccccgt tttctccacc aactggccta tcacgaagtt cacgatagca ccaacgatca
     7321 gggtagcgat aaacagtacc gcgatggcaa ttccatttcg aaccagttcg tcttcaaagc
     7381 ccgtaaacca gactgacagg taagtgtagt aatgactggc aacaaagaaa gcacaacccc
     7441 atgtcaccag cgataacgct tcacgaacaa agccgcggat caggctaacc agagaggaaa
     7501 aagcaatcac cgcgattatg gcgtaatcaa tccagaccat atgtgtccca cgattttacg
     7561 ccctgtcatc ctgttcgggg cgcattctaa cagaaaaaga aaacgtttgc gtagggattt
     7621 ccttcccacg catcaataaa aatggcgctg aaaaaatatt caacgccatc gactttttat
     7681 gcctttgcgg catcgtgcaa tgcgtgtcgg atgcggcgta aacgccttat ccgacctacg
     7741 gttctaacac tgcccaagcc tgataagacg cgccagcgtc gcatcaggca agactgtatt
     7801 aattcggcgt atagcccatt accacgccac ttaagccaga aagttgcttc aactcaccca
     7861 gcgaaccttt cagcttatct ttcgaggcat ccggcccaac cagaatacgg gtaattttac
     7921 cctgcactgg cgtggatggc gacgtataaa cccgataacc ggcaccgcgc agcttaccga
     7981 caatctcatt cactttatcg gcatttttca gcgcacccag ttgcacaaca taggctttac
     8041 ccgttggcgc tgctttttct tccacgaccg gcttcggttc tggcttcggc gcaggtggtg
     8101 cttcaacctt cggtttaggc ggctccaccg gtttcggctt cggtggggcg accggtgcag
     8161 gttccggttc aaactcggtg ttattggctg caatagtggc cggatcgagc gacggtgctg
     8221 cggcatcacc tgcccgcacc tcttccgctg cgccttccgg cggctgcgtc ggcaacgcct
     8281 gggtggcggc tggcatcata tcaggctcat cgcgatcgcc cgcttttgga accagcggga
     8341 tagccgcaaa ctcatcctga taatgttttt tctgcccgtc cagcagccct ggaagtacaa
     8401 tcacccccag cgccaccagc acgatcgtgc ccactaaccg attctgaaac ttacttgcca
     8461 ccgcttctcc tcgcgtcaat cacttccatg acatgtgcga ccgtgtggaa agaaccacac
     8521 accagcacgg tgtcttccgc tttagcgtcc gccattgcgg catcccatgc ctgcgcaacg
     8581 ctatcaaatg atttgccgtt acccaaatgc tcaagcagtt gttctgccgt ggcaccgcgc
     8641 ggcccttcca gtggcgcaca ataccagtca tcaaccacgc ttttcaacca ggccagagtt
     8701 ccggcaatat ctttatcatg tagcataccg ataaccgcca gcacgcgccc gtttttcggt
     8761 agcgctttca tacgcccggt gagatattcc gccgcatgtg gattatgcgc gacatcaaaa
     8821 ataacgcgtg gcgactcgct cacaatctgg aaacgtcccg gcaaaattgc gctggcaatc
     8881 ccgtcgcgaa tggcattttc actgacttcc agcccgctgg cacgcagtgc cgccagcgct
     8941 gttgcggcat tcggttgcgg gacaagcggc aacggcagat tttccagcgt gccgtgagca
     9001 tcgctaaacg cccagtcatg atcggtgacg gaatagttcc actcaacgcc ccgacgttgt
     9061 aacagtgcac ctttttcctg cgccacatca gcaatggtag aaggcatttc cggctcaccg
     9121 acaattgccg gttttgcgct gcgaaagatg cctgctttct cgcgaccaat actttcgcga
     9181 tctggcccca gccagtcggt atgatccagc gcaatactgg ttactaccgc gacatcggca
     9241 tcgacaatat tggttgcgtc cagacgaccg cccagcccta cttccagaat caccacgtca
     9301 agttgtgcct gtttgaacag ccacaacgcc gacagcgtac cgtactcgaa ataggtcagg
     9361 gaaatatcac cgcgtgccga ttcaatctcc gcaaaagagg cggtgtgggc cgattccggc
     9421 aattcctgcc cctgcacacg tacgcgctcg gtataacgca ccagatgcgg cgaactgtag
     9481 acgcccactt tgtaccctgc cgccatcaga atcgactcca gcgtacggca ggtggtgcct
     9541 ttgccattcg tacccgcaac ggtaaacaca aatggcgctg gtttcagaac gccaagacga
     9601 gccgcgacct ggctcacgcg ctcaaggccg agatcgatag ttttactgtg caggttttcc
     9661 agataagaaa gccacgaagc cagaggcgac gcggcttgag gagtgcgttt gataatcatg
     9721 gtatccgctg attcgttacg gtgagaatag caaaagggca gagccagtgg ccctgccctt
     9781 atcagttatc aggcctcagg ttcctgatcc ggtaccgggg gtaccactac gccttcacgc
     9841 ggcgcttcag gattcggcgc tggcagattc atcaacttcg ccagaatgct cgccagtttc
     9901 aggcgcattt ccggacgacg gacgatcatg tcgatcgcgc ctttctcgat caggaattca
     9961 ctgcgctgga atcctagcgg cagtttttcg cgaacggtct gttcgataac acgcggaccg
    10021 gcaaagccaa ttaacgcttt cggttcagcg atgttgagat cgcccagcat ggcgaaactt
    10081 gcagaaacac cgcccatcgt cgggtcggtc agcacggaga tgtacggcaa gccgcgctcc
    10141 tgcatttttg ccagtgccgc agaggttttc gccatctgca tcagcgacat cagtgcttcc
    10201 tgcatacgtg cgccaccaga ggcggagaag cagatcagcg ggcagttatc ttccagcgcc
    10261 tgctcaacgg cacgcacgaa acgtgcaccc acaacagacc ccattgaacc gcccataaag
    10321 gcgaactcga atgccgcagc gacaaccggc attccataca gagtgccttt catcaccacc
    10381 agcgcatctt tttcgccggt ttctttctgc gcagaagcca gacggtcttt atacttcttg
    10441 gagtcacgaa acttcagcac atctttcggc tcaagctcgc tacccatctc cacaaggctt
    10501 ccttcatcta acaggctatg caggcgatta cgcgctgtca tacgcatgtg atggtcacac
    10561 ttcggacaga cctcaagatt acgttccagc tcagcgcggt ataaaacctg accgcagcta
    10621 tcacacttag tccacacccc ttcaggaatg ctcgccttgc gggtgggagt aatgttgctt
    10681 ttaattcgtt caatccagct cattagggac ctttctgtct gaacctggtt cgatgccagt
    10741 tttatctttg gggacgcata atgccatttt tgcccccaac agaccatgaa tgttgcacat
    10801 taaaacataa cagcccgaaa ctttggataa aaaagtggtc gaaccgcgga gttacttttt
    10861 attttgcggc gcgtgccgca gcgcgtttgt gacgaataaa ttcgaaaacc ccaggcaata
    10921 ttgaaacaaa aataatgcca acaatcatca gcttaaggtt gctctgaatg aaggggagca
    10981 tgccgaagaa ataacccgcg taggtaaaaa tcagtaccca caacagtgcg ccgatcacgt
    11041 tataagcggc gaaatgacgg tacgacatgt ggcccattcc cgcaacaaac ggcgcgaacg
    11101 ttctgacgat cggtacaaaa cgggcgagaa taatcgtttt gccgccatgt ctctcgtaaa
    11161 actgatgagt cttatcaaga taactgcaac gaaagatttt cgaatccggg ttactgaaca
    11221 gtttgtcacc aaataaccgt ccaatggtat agttcactgc atcaccgaca atagcggcaa
    11281 tcaacataag caccaccatc atatggacat tgagatcgtt agtttccagc gacgccaatg
    11341 caccagcaac aaacagcagc gaatcaccag gcaggaatgg cgttaccacc agccccgttt
    11401 cgcagaacag aatcaaaaac agaatggcat aaacccagac gccgtactcc gcgaccagct
    11461 ctgccagatg cacgtcaatg tgcaggataa aatcgatgag gaaataaatc aggtccatat
    11521 ttgcctatgc cttgtactcg tcatctttca ggctgtaact acgttggcta cagcctgaaa
    11581 tgttccgagc attattctcg ttagtccgcc agaaatagcg ggcccattgg cggttttgga
    11641 agatcatacc ggtcagggta atccaccgcg accagataca gcccttccgc ttttgccgtt
    11701 gctgccgcca gcgttctgtc ctttgccgcc agcaactctg ctatccagct ctccggctgg
    11761 ttgtgggcac cgacttccat caggctgccg acaatattcc tgaccatatg atgtacaaag
    11821 gcattcgctt tgatatctac caccacataa ggaccgtgac gcgtgacatt aatatgcata
    11881 acgttgcgcc acggggttcg ggactggcac tgcaccgcac ggaacgaggt gaaatcattc
    11941 tcgcccagca agcattgcgc agcccgatgc atccgttcag cgtccagcgg ttcgtaaaaa
    12001 tgggttaccc ctttactcag taccgccggg cgcagccgat gattgtagat gatgtagcga
    12061 taacggcgag ccgtggcgct aaatcgggca tgaaaatcat caggtacagc tttaacccaa
    12121 cgcacggcga tgtcatcagg taaattcgca tttacgccca aggtccacgc cgcgtctttg
    12181 cgcggcgcgg ttgtttcgaa atgcaccacc tgcccggtac cgtgtacccc tgcgtcagta
    12241 cgcccggcgc agaagacggt gatgggttcg ttcgccacct gggagagcgc cttttccagc
    12301 ttctcctgca cactgcggac ttcattctgc cgttgccagc cgtaatactt actgccgtcg
    12361 tactcaatgc ccagcgcaat tttataaact ggcagttgtt gctggtcgga cattagtaca
    12421 gatactcctg caccagtttc tcggcgattt ttactgccat cagcgcgccg ccaaagcgaa
    12481 cgttatcggc caccgaccag aactggactt gctccggcat accgtagtca ttacgcacgc
    12541 agccaacaga aagatgcgga gtacccgaag catcccctac ctgagtcggg aattcgttct
    12601 cttcagagag cacaatatct tcgccctgag caaacgcatc acgcgcttct tctgccgcca
    12661 gcggacgcag tgcttcaaag ttgaccatct gggcatgacc gtagaatacc ggtgcctgga
    12721 cgacgcttgc cgaaatcatc agcccttcgt cctgcaggat tttgcgtact tcgtcaacga
    12781 tacgacgttc ttcacgcacg ctaccttcgc tatccggcaa caacggcagc atattgaacg
    12841 ccagttgacg accgaagaaa tcttcttcgt caatcggaat gccgttgagc aatttcgcac
    12901 tctgccccgc taacgcatcg accgcttttt tgccctgggc ggaggctgaa atcaggctgg
    12961 taacactgat acgcgacaaa ccgccctgat cgattaacgg tttcagtgcc gccagcagct
    13021 ggctggtcag actgtctggt acggcgatga cattccggtt gcggtaatcg gtcagtacaa
    13081 acgggtttac ttccggcacc accagcggta cgtcgggttc gagagcaaac agtccgctgc
    13141 tgtcgatcac caggcaacct gagttggtcg cttcttcaac ccaggcagcg gtagcttctt
    13201 tgcctgcgac aaaaaatgcc agctgcgcct gcgtccagtc gaattcagcg gcatcctgca
    13261 cggtgattgt tttaccacca aagcgcagtt gttcgcctgc gctttcgtta cgtgccagtg
    13321 cataaatttc cccaaccggg aactgacgtt cagccagcgt ttcaagcagg gcttcgccca
    13381 cagcgccagt tgcgcccagg acggcaatgt tccagccttc agacatggtg gtttactcca
    13441 gaaatagcaa agctccctgc caatgttaca gcagagagca tgaagaagag attaacgtgc
    13501 cggatgatga acggcgttaa aacccagttt acacagcaat gatgccgcac tggcgtcatc
    13561 acaaattaca tacagagatg accattcacg gcgctcaaga tagtttttgc gcagtttatc
    13621 gaactcaccc ggtatcccgg cgactttacg cagcggtgcg tcatcgcggc gcacatcata
    13681 caccaaatgc accagccttt tcagcgtcgg ttgatcgagc gggccatgca gcgtaatgcg
    13741 accaaactct ggcgcgggca gtaatgtatc cagcgcaacg tgctgttcat gcccaataaa
    13801 cttgctataa gcttcaaaca cttgcgtagt accgcgtgct ttaccttcca gggtataacc
    13861 tgcgatatgc ggcgtgccga tatccacttt tgtcagcaac tccacgttaa gttccggttc
    13921 gccttcccag acatccagca ctacgcttaa cttctggcct tcattcaggc aggtcagcaa
    13981 cgcagtatta tcgacgactg cgccacggca ggcgttaatc agaatcgctc ccggcttcag
    14041 gctacggatc agtttttcat ccgccagatg cagcgttttg tacggcccat ctttgaagag
    14101 tggcgtatgg aaagtcagaa tatccgcatg ctgaactaat tcatccagcg agcggaaatc
    14161 accctcatcc ccacggtcag cgcgaggcgg atcgcaaagc aatgttttaa tccctaacgc
    14221 ttccagtcgc gcctgcaatc gacgtccaac gttaccaacg cccacgatcc ccaccgtacg
    14281 gtcgtgcagt gaaaatccat cgcgttcggc aagcatcagt agtgaggaga aaacatattc
    14341 caccaccgca atcgcattac agccaggtgc agcggaaaaa ccaattcccg cctgcttcaa
    14401 ccatgcttca tcgacatggt cggtccccgc agtggcagtg ccaacaaatt taatgggttt
    14461 tcctgccagc aaagattcat tcactttcgt gaccgaacgc accatcagcg catccgcgtc
    14521 tgccagttga gcgacggcga ttggacgccc gggaaccgcg gtcacctcac ccaaacggct
    14581 aaataagtcg cgggcataag gcatattttc atcaacaagg attttcacgt ttgtgttacc
    14641 tgtatgagac gagagttaac cggacaagtg tgccataatc tcgcggccag gcatacttgc
    14701 gaagatttca ggtataagga tacgtaatga tacaacctat ttccggccct cctcctgggc
    14761 aaccaccagg tcagggagat aacctgccgt ctggcgcggg caatcagcct ttatccagtc
    14821 agcaacgtac ttcgctggaa agcttaatga cgaaagtgac ctcactgacg caacagcaaa
    14881 gagcagaact gtgggcgggt atcaggcacg atattggtct gtcgggagat tcaccgctgc
    14941 tttcgcgtca cttccctgcc gctgagcata atctggcgca acgtctgctg gccgcgcaaa
    15001 aaagccattc tgcccgccag cttttagcgc aattagggga gtatttacgt ctggggaata
    15061 atcgtcaggc ggtcacggat tatatccgtc ataactttgg tcagacgccg ctgaatcagc
    15121 tctcaccgga gcaattaaaa accattctca ccctgttgca ggaagggaag atggttattc
    15181 cgcaaccgca gcagcgcgag gcgaccgacc gtcctttatt accggcggag cacaatgcgc
    15241 taaaacagct ggtgaccaaa cttgcggcgg caacggggga acccagcaaa cagatctggc
    15301 aatcgatgct ggaactttcc ggggtgaaag atggcgagtt aattccagcg aaactgttta
    15361 accatctggt gacctggcta caggcgcgtc agacgctaag ccagcaaaat acgccgacgc
    15421 tggaatcact acagatggcg ctaaaacaac ctttagatgc cagtgaactg gcggcgttat
    15481 cggcatatat ccagcaaaaa tatggccttt ctgcgcaatc atcgctttct tctgcccagg
    15541 ccgaggatat tcttaatcag ctttatcaac ggcgggttaa agggattgat ccgcgtgata
    15601 tgcaaccgct gcttaatcct tttccaccga tgatggacac gttgcaaaat atggcaacgc
    15661 gtcccgcgct gtggatactg ttagtcgcga ttatcctgat gctggtctgg ctggttcgtt
    15721 aaccccgacg aaatgacagt atcgtgacaa taatacccag caccgcagag atcgccccgg
    15781 caagaaatac cgaagagtaa ccaaacgtgg tcgccagcat tcccgccagc ggcccggaga
    15841 cgccgagggc gatatcctga aacgcggcgt aaccgcccag tgcggtgccg cgaacttgtg
    15901 aggggacgcg tttaaccacc tccacgccca gcgcaggaaa gataagcgaa catccggctc
    15961 cggttaacgc cgcgcccgct aatgcgaccc atgcacctgg ggcttgccag agcagcaaca
    16021 agcccaccgt ttctacaagc agagagacaa tcgccacttt cacgccgcca aaacggtccg
    16081 gcatccagcc aaacatgacg cgcatcacga caaatgcgcc gccaaacgcg gtaagagtaa
    16141 agcccgccat cgcccatcct ttgctggcaa agtagagcga aacgaaagtc ccgataaccg
    16201 caaaaccaac gccttgtagt gccagcccta accctggttt ccagataagc ccgacaacgc
    16261 tccacagcga tggacgttct cccgccaggg ccggtacttt gcgcactgtg ccgttacagg
    16321 cccacgccag taagggtaat accattgtgg tgatcgccag tgcggcaaaa ccgtaatggc
    16381 tatgaatcaa caggccaagc ggcgcaccaa cagcgagggc accgtaaatc gccattccgt
    16441 tccatgacat cactttgcca gagtgttttg cccctacgat gcctaatccc caggtcagag
    16501 cgcctgtcag taactggctt tcaccaaagc caagaatcaa acgcccgacg accaacaggg
    16561 caaatttcaa cggtgcggag acaggcaaaa tcgccgccag cagcaacgcg ccgccagcca
    16621 gaccacaagc taacatcccc tgaagcgccg aacgttttgc accatattga tcggccagtc
    16681 gcccggcata gccacgcgtc agcaccgtag ccagaaactg aatcccgacg gcaatcccga
    16741 ccatggtatt gccatagccc agatcatgat gaacaaacag cgggataacc ggcaacggca
    16801 gccctacggt catgtaggtg agaaaaaccg caaaagcgat gcggaagagc gaaaaattgg
    16861 cagaagatcg tgtttcggtt tggcttacag cagtcatgca ttactccaga atgcagcgca
    16921 aggcgaggag tatccccgtc tcatctctct ggtttcaggg ttacagtgcg ttggcaggat
    16981 ttaacgcgta cgtcttttca gaaggaaatc gacaaagcgg gaagtttgcc tggaactggc
    17041 ggcgattgtc aatgatgtga aaaagggaac catcaggttc ccttttgcgt tagtgccgga
    17101 ggccgcattg gcgcgtaacg tcggatgcga cgctggcgcg tcttatccga cctactccga
    17161 ctactccgac tactccgact actccgacta ctccgactac tccgactact ccgacctact
    17221 gcgaattaat ctttcagctt gcgcattacc agcgtggcgt tggtgccgcc gaagccgaag
    17281 ctgttagaca taacggtggt cagttcgcga tcggtcgttt cggtcacgat gttcagacct
    17341 gccgcctgct cgtccagctc ttcaatgttg atgctcgggg cgataaagcc gtgttccagc
    17401 atcagcagag agtagatagc ttcctgtacg ccagcagcgc ccagagagtg accggtcatg
    17461 gctttggttg cagaaatcgc cgggctctta tcgccgaaca cttcacggat agctgccagt
    17521 tctttcacgt cgccaaccgg agtcgaagta ccatgagagt tcaggtaatc gattggggta
    17581 tcaacgccgt gcatcgccat cttcatgcag cgtactgcgc cttcgccaga cggagcaacc
    17641 atgtctgcac catcagaggt tgcgccgtag ccaacgattt cagcatagat gtgagcacca
    17701 cgcgccagcg cgtgttccag ctcttcaacc actaccatac cgccgccgcc agcgataacg
    17761 aaaccgtcac ggtgagcgtc gtaagtacgg gaggcttttt ccggggtgtc gttgtattta
    17821 gtagacagcg cacccatcgc gtcgaattcg caggccattt cccagcacag ctcttcgccg
    17881 ccgccagcaa acacgatgtc ctgtttgccc agttggatct gctctactgc gttaccgata
    17941 cagtgtgcgg aagtcgcaca cgcggagctg atggagtagt taacgccatg aattttaaac
    18001 ggcgtggcga ggcaggcaga aacgccggat gccatcgctt tggtgaccac atacgggcca
    18061 accgctttca ggccgcgcgg gccgcgcatt gcgtcagcgc cgaatacctg gaaacgcggg
    18121 gaaccgccgc cagaacctgc aatcaggcca acgcgcgggt tattctggta agcttccgga
    18181 gaaaggcccg catctgcgat tgcctgctcc atagaaagga atgcataaat ggatgcgtcg
    18241 ctcataaagc gcacaacttt gcggtcaatg aggccagtgg tatccagttt tacgttgccc
    18301 cagacgtggc tacgcatgcc ggaatcctta agctcctgag agaaagtgat ccctgaacgt
    18361 ccttcacgca gagatgccag gacttcctgc tggttattac cgatgctgga aacaatgccc
    18421 aggccagtaa tcactgcacg tttcattcaa tacctctgta agtcgcacat agagtaagtt
    18481 tcgaatgcac aatagcgtac acttgtacgc cgaacaagtc cgatcagcca tttaatagag
    18541 aaatttgcgc agccttacac acatcgctaa gatcgagcca ccgcctgtaa gacgagtaac
    18601 ttacgtgaaa cactactcca tacaacctgc caacctcgaa tttaatgctg agggtacacc
    18661 tgtttcccga gattttgacg atgtctattt ttccaacgat aacgggctgg aagagacgcg
    18721 ttatgttttt ctgggaggca accaattaga ggtacgcttt cctgagcatc cacatcctct
    18781 gtttgtggta gcagagagcg gcttcggcac cggattaaac ttcctgacgc tatggcaggc
    18841 atttgatcag tttcgcgaag cgcatccgca agcgcaatta caacgcttac atttcattag
    18901 ttttgagaaa tttcccctca cccgtgcgga tttagcctta gcgcatcaac actggccgga
    18961 actggctccg tgggcagaac aacttcaggc gcagtggcca atgcccttgc ccggttgcca
    19021 tcgtttattg ctcgatgaag gccgcgtgac gctggattta tggtttggcg atattaacga
    19081 actgaccagc caacaggacg attcgctaaa tcaaaaagta gatgcctggt ttctggacgg
    19141 ctttgcgcca gcgaaaaacc cggatatgtg gacgcaaaat ctgtttaacg ccatggcaag
    19201 gttggcgcgt ccgggcggca cgctggcgac atttacgtct gccggttttg tccgccgcgg
    19261 tttgcaggac gccggattca cgatgcaaaa acgtaagggc tttgggcgca aacgggaaat
    19321 gctttgcggg gtgatggaac agacattacc gctcccctgc tccgcgccgt ggtttaaccg
    19381 cacgggcagc agcaaacggg aagcggcgat tatcggcggt ggtattgcct gcgcgttgtt
    19441 gtcgctggcg ctattacggc gcggctggca ggtaacgctt tattgcgcgg atgaggcccc
    19501 cgcactgggt gcttccggca atcgccaggg ggcgctgtat ccgttattaa gcaaacacga
    19561 tgaggcgcta aaccgctttt tctctaatgc gtttactttt gctcgtcggt tttacgacca
    19621 attacccgtt aaatttgatc atgactggtg cggcgtcacg cagttaggct gggatgagaa
    19681 aagccagcat aaaatcgcac agatgttgtc aatggattta cccgcagaac tggctgtagc
    19741 cgttgaggca aatgcggttg aacaaattac gggcgttgcg acaaattgca gcggcattac
    19801 ttatccgcaa ggtggttggc tgtgcccagc agaactgacc cgtaatgtgc tggaactggc
    19861 gcaacagcag ggtttgcaga tttattatca atatcagtta cagaatttat cccgtaagga
    19921 tgactgttgg ttgttgaatt ttgcaggaga tcagcaagca acacacagcg tagtggtact
    19981 ggcgaacggg catcaaatca gccgattcag ccaaacgtcg actctcccgg tgtattcggt
    20041 tgccgggcag gtcagccata ttccgacaac gccggaattg gcagagctga agcaggtgct
    20101 gtgctatgac ggttatctca cgccacaaaa tccggcgaat caacatcatt gtattggtgc
    20161 cagttatcat cgcggcagcg aagatacggc gtacagtgag gacgatcagc agcagaatcg
    20221 ccagcggttg attgattgtt tcccgcaggc acagtgggca aaagaggttg atgtcagtga
    20281 taaagaggcg cgctgcggtg tgcgttgtgc cacccgcgat catctgccaa tggtaggcaa
    20341 tgttcccgat tatgaggcaa cactcgtgga atatgcgtcg ttggcggagc agaaagatga
    20401 ggcggtaagc gcgccggttt ttgacgatct ctttatgttt gcggctttag gttctcgcgg
    20461 tttgtgttct gccccgctgt gtgccgagat tctggcggcg cagatgagcg acgaaccgat
    20521 tccgatggat gccagtacgc tggcggcgtt aaacccgaat cggttatggg tgcggaaatt
    20581 gttgaagggt aaagcggtta aggcggggta atctgctctc gcagtgtttg ccggatgcgg
    20641 cgtgaacgcc ttatccgacc tacgtgtgac gtgtaggcct gataagacgc gataagcgtc
    20701 gcatcaggca tggtgctcca gacgccgcaa cttactgctg tgacgcctgc tgaaacaggt
    20761 tttcccacat atcggtcacc agcgcctggt cacgcggcga caattcaccg gcaccaatgg
    20821 ctttttccag actctggcta acggtcgtat gcaccgcctg agcggagtgg tcatcaccac
    20881 tttccagttc tgcgatggct aatgtcaggt ggccacgcaa atacccactg gcaaacaact
    20941 catcatcact ggcatggtca atcatgccgt cgattaatgc cagaatgcgt gattcaaact
    21001 ccgcgatcat cttctttcct cattgtaaaa cgtggcaccg gcttcagttg agcgcttccg
    21061 gccacgggaa ctgttccgcc gtaagttccg gcgtgtgata ataattttgt agtgctttaa
    21121 tgaagcgtgc cggacgctcg ggaatgccat ttgtcagata atccatcacc tgcgcatgta
    21181 cccgccgttg aaacaccaca cgatccggtt cgaagtcgcc ttccagattg tcgcaactga
    21241 cgttaaacgg atatcccgcc gccacacaga acaaccagtc gagtgcctgc ggcttcactt
    21301 caacatcttc aaactggctt tgcgtttggg catcacgccc atccgggcaa taccagtagc
    21361 cgaaatcaac cagctcacgg cgcgcttttc cggcaataca ccagtgcgaa atctcatgaa
    21421 ttgcgctggc ataaaagccg tgggcaaaga caattcggtt atacggtact tccgcatcag
    21481 caggaagata gatcggttcg tcgtcgcctt taatcagacg ggtattaaat tcatcggcaa
    21541 agcagctatt aaaaatttca atcaactgct catagtggtg tgtactgttc attagttcat
    21601 ccccaaccag tggaggatct cctgtccgtg gctatcgtaa agtaatttgg cactcatcac
    21661 cgccgagacg ataacgatca tcgggcggat cagtttttgt cctttgctca gcaccagtct
    21721 tgaacccatg cgcgcgccga ggaactgccc caccagcatg acaaagcccg tcgcccaaat
    21781 cactttgccg ccgagaataa acagcagcag accgccaatg tttgacgttg cgttgagtaa
    21841 tttggcgtga gccgtggctt tggcgaggtt gaatccgcac agcgtaacga aggccagcgc
    21901 gtaaaacgat ccggcagccg ggccaaagaa tccgtcataa aaaccgacgc agccaccggc
    21961 aattaacgcg aagggtaaac cgtacatccg gcgctggcga tcttcttcac ccagctttgg
    22021 catcagcaag aaatagaggc cgatacaaat taccagaatg ggcaaaatct gccgtaagac
    22081 atcagcctga acgtattgca ccagcagtgc gccgctcatt gagccggcaa aggtcatggc
    22141 gatattgagt ttctgatcgc ttaaactaac cactttgcgg cgaataaagt agatagtggc
    22201 ggaaatagag ccgccgcagg cttgcagttt attggttgcc agcgcgttag cgggagacat
    22261 tcccgcagcc atcaatgccg gaatggtgag taacccaccg ccaccggcaa tcgagtcgat
    22321 aaatcccgcc agcatggcga caaaaaagag aactcccagc aacagcgggg aaaccataaa
    22381 caggctatta aacgtttcca ttagatcacg tgctcatcca gtagcgcctg gcaggaaggc
    22441 ggcaacggag gcggtgtctt cttctcaggc tttgttgttc ctggttttgg aggtgcaaac
    22501 cagctttgca gttctgcccc gcaaccatcg cctggtggcg gtaaaggttg atcttcacac
    22561 tccagactat cggcagggca acgtaatcgt acatgcatat gcgcgcgatg ctggaaccag
    22621 ggtcgcactt tgcgcaacca gtcgcgatcg gtgcccgcat caaggcaaag ttgttgttta
    22681 atcgccggat taacaaaaat gcgcgtgacg tctttgtcct gggcggcgag tttgatcaag
    22741 ctgaaaattt ctggcttcca cagcgtggag acaacgtgtt taccgtcgcg ggaaactaag
    22801 tccagtgctt gcgggcgcaa gagctgcgcg gaggtccagc gagttttcgg cagttgcaga
    22861 aagatatcga catccagtcc ggtctggtgg ctggcatgac cgccgttgaa acgcccacca
    22921 gcgggcatcc ccatatcgcc aatcagcacc gtacccatgc ccagattgct cacctggcta
    22981 ctcagacgct ggataaacat caccagatcc gggtgaccga aatagcgacg ctgatcggta
    23041 cgcatgacct gataatgttc ggactgtatc ggcaacgtgt cagcgccgac aatacagcca
    23101 ttagaaaaac tgcctatcga ttgtgcgcta cccggcacag gttgggttat tttttgccac
    23161 ggcgtcgctg ccaagctagc gctactggca agcagagcca gcagcgcaat cgcggttttg
    23221 ttcatttttt accagcgtgg aatatcagtc ttcacatcgg cattttgcgc ccgctgccgt
    23281 aacaggtgat ccattaaaac gatcgccagc atcgcttcag cgatcggcac tgcacggatc
    23341 ccgacacagg gatcgtgacg acctttggtg atcatctcaa cttcttcgcc aaagcggtta
    23401 atagtgcgcc ccggcacggt aatgctggag gtcggtttca gcgccatatg agcaatgatt
    23461 tgctgcccgc tgctgatacc gccgagaatg ccgcccgcat ggttgctctg gaaaccgtct
    23521 ttggtgattt cgtcgcggtt ctggctgccg cgcagcgcca ctacgtcaaa gccgtcgcca
    23581 atttccacgc ctttcaccgc gttgatgctc atcagcgcat gggcgatgtc ggcatccagg
    23641 cggtcaaata ccggctcgcc aagtccggcg gggacgccac tggcaacaac ggtgactttc
    23701 gcgccgatgg agtcgccctc ttttttcagc gcgcgcatca gttcatctaa cgcgtcgatt
    23761 ttgtccggat ccgggcagaa gaacggattt tgttcaacca gcgaccagtc tttgatttcc
    23821 agcggaatgt cgcccatctg ggtcaagcag ccgcggattt caataccaaa tttctcggcg
    23881 agatattttt tggcaatcgc ccctgccgcc acgcgcatgg cggtttcgcg ggcggaagaa
    23941 cgtccaccgc cgcgataatc gcgcagaccg tatttttgtt cgtaggtgta atcagcatgg
    24001 cctgggcgga aaacgtcttt aattgcgctg taatcctgag agcgctggtc agtgttttcg
    24061 atcaacaagc caatgctggt gccagtagta acgccttcaa aaacaccaga gagaattttg
    24121 acctgatccg gctcgcggcg ctgggtggta tagcgcgatg tcccagggcg acgacggtcg
    24181 aggtcatgtt gcaggtccgc ttccgtcagc agaatgcctg gcggaacacc atcgacgatg
    24241 cagccgagcg ccagcccatg cgattcgccg aaggtggtta cgcgaaagag ttgtccaatt
    24301 gtgtttccag ccatcacggc tccgttattg ttgtgtttgc gtgtttactt aatctttata
    24361 aatcgcgaaa tattcacggg ccgccagcag ctgctctttg gtgagcataa acacaccatc
    24421 gccgccgtta tcaaactcca gccaggtgaa cggaacatcc ggatattgtt ccataagatg
    24481 taccatgctg ttgccgactt cacaaatcaa cacgccatca tcagcaaggt aatctgccgc
    24541 gtttccaaga atgcgacgcg tcagtttaag gccgtcggtg ccagatgcca ggcccagttc
    24601 cggctcgtga cggtattcgt ttggcaggtc ggacatatct tccgcgtcga catacggcgg
    24661 gttagtgaca atcagatcgt actgcacttt cggcaagtcg cggaacagat cggaacgaat
    24721 cggaatgacg ttgtggatca gaccgtgttc ttcgatgttc tgttcagcaa ccgccagcgc
    24781 gtcgggagag atatccaccg cgtcgacttc tgcatccggg aaggcataag cacaggcaat
    24841 ggcgatgcag ccgctaccgg tacacatatc taaaatatgc tgcggttgtt tgctgatgag
    24901 tccggcaaat ttattgttga tcagttcacc aatcggcgag cgcggcacca gcacgcgttc
    24961 atcgacgtaa aattcatgac cgcagaacca ggctttgttg gtcaggtaag ccaccggaat
    25021 gcgctcgttg acgcggcgga tcacgcgttc aacaatacga tgtttttcgc tggaggtcag
    25081 acgcgcggtg cgcatatctt ccgggatatc cagcggcagg tagagcgatg gtaacaccag
    25141 ctgtacggct tcatcccacg ggttatcggt gccgtgaccg taccagatat ttgccgcgct
    25201 gaagcggctc actgaccagc gcaacatgtc ctgaatggtt tgcagctcat ttactgcttc
    25261 atcaacgaaa attttatcca cgtattcctc cagggcatga tcgcaataat ttcggcggct
    25321 agtttgccat gaagatgacg ataaatcagc attcacgcgc ggtgagtgag gaaaaatacg
    25381 tttaaaacga tcgattgtgc tacgagtcgg gtaaactgta ggaaaattag aaatagagac
    25441 agataaatga aaaagaaaac aacacttagc gaggaggacc aggctctgtt tcgccagttg
    25501 atggcgggga ctcgcaagat taagcaggac acgattgtcc accgaccgca gcgtaaaaaa
    25561 atcagcgaag tgccggtgaa acgcttgatc caggagcagg ctgatgccag ccattatttc
    25621 tccgatgagt ttcagccgtt attaaatacc gaaggtccgg tgaaatatgt tcgcccggat
    25681 gtcagccatt tcgaggcgaa gaaactgcgc cgcggcgatt attcaccgga attgtttttg
    25741 gatttacacg gtctgacgca gctgcaggcc aagcaggaac tgggcgcgtt gattgccgcc
    25801 tgtcgccgcg aacatgtgtt ttgtgcctgc gtgatgcatg ggcacgggaa gcatattttg
    25861 aagcaacaaa caccgctgtg gctggcacaa catccgcatg tgatggcatt tcaccaggca
    25921 ccgaaagagt acggcggtga tgcggctttg ttggtgttga ttgaagtgga agagtggttg
    25981 ccgccggagt tgccctgagg agttgagcgt gttgtgccag gagcgcggaa tggcaggggc
    26041 gaatgaccct gccaggtgag aaaaggtgcg ctaatacata ttcagtatgt aagtgaatcg
    26101 gcaggtagaa tctatgggct gtcgagatgt cgtgtccacg cgtagcttca tatcatgcag
    26161 ttgcctggac gtaatcggtt gcggctcagg cgtcaccgtc actttggcat tatcacagcc
    26221 ggaagtcgag aaggtcatct gctgtgcgat aaaacggcag tagacaccac gctcatcaaa
    26281 attattcccg ttacacagtt tgccattttc catcaggaaa gtgggcagtg tttgctcaat
    26341 aacaccacct gttgactgca acctgacgac tttgttatgt acaacataat tttcattcca
    26401 ggaaacatca tacggtgcca ggggcaccgc cggacatgaa gaactcacct tcgttgggcc
    26461 aatatagtca ggaaaatcat catcatacgt tttagcgaag ggatctctac tcttaacaaa
    26521 cgacgacaca atccacggac aaccatgtaa attcccatcg taataaacaa acatcttta
//
LOCUS       DAJVUJ010000043        25510 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.043, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000043 DAJVUJ010000000
VERSION     DAJVUJ010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25510
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.043"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..184
                     /locus_tag="OQQ48_001643"
     CDS             <1..184
                     /locus_tag="OQQ48_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HCS1952453.1"
                     /translation="ACMAITNLTAILLLSPVVHTIASDYLRQRKLGVRPVFDPLRYPD
                     IGRQLSPDAWDDVSQE"
     gene            254..1030
                     /gene="yaaA"
                     /locus_tag="OQQ48_001644"
     CDS             254..1030
                     /gene="yaaA"
                     /locus_tag="OQQ48_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HCS1952454.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(1183..1479)
                     /locus_tag="OQQ48_001645"
     CDS             complement(1183..1479)
                     /locus_tag="OQQ48_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCS1952455.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGNRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(1693..2979)
                     /gene="thrC"
                     /locus_tag="OQQ48_001646"
     CDS             complement(1693..2979)
                     /gene="thrC"
                     /locus_tag="OQQ48_001646"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HCS1952456.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(2980..3912)
                     /gene="thrB"
                     /locus_tag="OQQ48_001647"
     CDS             complement(2980..3912)
                     /gene="thrB"
                     /locus_tag="OQQ48_001647"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HCS1952457.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(3914..6376)
                     /gene="thrA"
                     /locus_tag="OQQ48_001648"
     CDS             complement(3914..6376)
                     /gene="thrA"
                     /locus_tag="OQQ48_001648"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HCS1952458.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTSGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGACRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(6401..6520)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(6456..6521)
                     /gene="thrL"
                     /locus_tag="OQQ48_001649"
     CDS             complement(6456..6521)
                     /gene="thrL"
                     /locus_tag="OQQ48_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HCS1952459.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(6735..7421)
                     /locus_tag="OQQ48_001650"
     CDS             complement(6735..7421)
                     /locus_tag="OQQ48_001650"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCS1952460.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLVESLHDVDFTVATTARSRAKYHYYATPVELVLLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPTKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(7821..7961)
                     /locus_tag="OQQ48_001651"
     CDS             complement(7821..7961)
                     /locus_tag="OQQ48_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1952461.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            8057..8773
                     /gene="arcA"
                     /locus_tag="OQQ48_001652"
     CDS             8057..8773
                     /gene="arcA"
                     /locus_tag="OQQ48_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HCS1952462.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(8833..10185)
                     /gene="creD"
                     /locus_tag="OQQ48_001653"
     CDS             complement(8833..10185)
                     /gene="creD"
                     /locus_tag="OQQ48_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HCS1952463.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMPLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDWATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTMDDQLRIWK
                     "
     gene            complement(10243..11667)
                     /gene="creC"
                     /locus_tag="OQQ48_001654"
     CDS             complement(10243..11667)
                     /gene="creC"
                     /locus_tag="OQQ48_001654"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HCS1952464.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDSQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPELGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTVVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLEQALGNLLDNAIDFTPESGRITLS
                     AEVDQEHVALKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(11667..12356)
                     /gene="creB"
                     /locus_tag="OQQ48_001655"
     CDS             complement(11667..12356)
                     /gene="creB"
                     /locus_tag="OQQ48_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HCS1952465.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QLVPDVMILDVGLPDISGFELCSQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLHAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(12369..12842)
                     /gene="creA"
                     /locus_tag="OQQ48_001656"
     CDS             complement(12369..12842)
                     /gene="creA"
                     /locus_tag="OQQ48_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HCS1952466.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            13053..13922
                     /gene="robA"
                     /locus_tag="OQQ48_001657"
     CDS             13053..13922
                     /gene="robA"
                     /locus_tag="OQQ48_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HCS1952467.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(13919..14566)
                     /gene="gpmB"
                     /locus_tag="OQQ48_001658"
     CDS             complement(13919..14566)
                     /gene="gpmB"
                     /locus_tag="OQQ48_001658"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HCS1952468.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAARRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            14618..15139
                     /gene="yjjX"
                     /locus_tag="OQQ48_001659"
     CDS             14618..15139
                     /gene="yjjX"
                     /locus_tag="OQQ48_001659"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HCS1952469.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENTSQRGEARSA
                     TLPLPAVILQKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            complement(15224..15550)
                     /gene="trpR"
                     /locus_tag="OQQ48_001660"
     CDS             complement(15224..15550)
                     /gene="trpR"
                     /locus_tag="OQQ48_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HCS1952470.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(15640..17577)
                     /gene="sltY"
                     /locus_tag="OQQ48_001661"
     CDS             complement(15640..17577)
                     /gene="sltY"
                     /locus_tag="OQQ48_001661"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HCS1952471.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            17788..19455
                     /gene="ettA"
                     /locus_tag="OQQ48_001662"
     CDS             17788..19455
                     /gene="ettA"
                     /locus_tag="OQQ48_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HCS1952472.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(19762..20994)
                     /gene="nadR"
                     /locus_tag="OQQ48_001663"
     CDS             complement(19762..20994)
                     /gene="nadR"
                     /locus_tag="OQQ48_001663"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HCS1952473.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIDTVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(21015..22397)
                     /gene="radA"
                     /locus_tag="OQQ48_001664"
     CDS             complement(21015..22397)
                     /gene="radA"
                     /locus_tag="OQQ48_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCS1952474.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(22446..23414)
                     /gene="serB"
                     /locus_tag="OQQ48_001665"
     CDS             complement(22446..23414)
                     /gene="serB"
                     /locus_tag="OQQ48_001665"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HCS1952475.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            23520..24164
                     /locus_tag="OQQ48_001666"
     CDS             23520..24164
                     /locus_tag="OQQ48_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HCS1952476.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            24192..25208
                     /gene="lplA"
                     /locus_tag="OQQ48_001667"
     CDS             24192..25208
                     /gene="lplA"
                     /locus_tag="OQQ48_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HCS1952477.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITKAFFAHYGERVEA
                     EIISPDKTPDLPNFAEIFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
     gene            25240..25389
                     /locus_tag="OQQ48_001668"
     CDS             25240..25389
                     /locus_tag="OQQ48_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1952478.1"
                     /translation="MIDLENQEREIINLMLSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLK"
BASE COUNT         6131 a         7029 c         6336 g         6014 t
ORIGIN      
        1 ggcctgcatg gctattacca atttgaccgc cattttactg ctctcgcctg tggttcatac
       61 cattgccagt gattatctac gccagcgtaa actcggcgtg cgcccggtgt ttgatccgtt
      121 gcgttatccg gatatcggtc gccagctttc tccggacgcg tgggatgatg tttcgcagga
      181 gtaatcacaa ctatcgatca actcattctc tttttttgct aaagtcggca taaatttcct
      241 gcaaggactg gatatgctga ttcttatttc acctgcgaaa acgcttgatt accaaagccc
      301 gttgaccacc acgcgctata cgctgccgga gctgttagac aattcccagc agttgatcca
      361 tgaagcgcgg aaactgacgc ctccgcagat tagcacgctg atgcgcatca gcgacaaact
      421 ggcgggtatc aacgccgctc gctttcatga ctggcagcca gatttcacgc cggcgaatgc
      481 ccgccaggcg attctggcgt ttaaaggtga tgtctacacc ggcttgcagg ccgaaacctt
      541 cagcgaagac gatttcgatt ttgcccaaca gcatttgcga atgctttccg gcttgtatgg
      601 cgtactgcgc ccgctcgatt taatgcagcc ttatcgtctg gaaatgggga tccgtcttga
      661 gaatgcccga gggaaagatc tgtatcaatt ctggggagat atcatcacca acaagctgaa
      721 cgaggcgctc gcagcacaag gcgataatgt ggtgattaat ctggcgtcag atgagtattt
      781 taaatcggtg aagccgaaga aattgaatgc cgagattatc aagccggtgt tcctcgatga
      841 gaagaacggc aaatttaaga tcatcagctt ctacgctaag aaagcacgcg gtctgatgag
      901 tcgtttcatt attgaaaatc ggctgaccaa accagagcaa ctgactggtt ttaatagcga
      961 aggttacttc tttgatgaag cttcctccag caatggcgaa ctggtgttta aacgctacga
     1021 gcagcgttaa gtttgcagtc aatgccggat gcggcgtgaa cgccttatcc ggcctacaaa
     1081 agcatgcaga ttcaatatat tgcagaattc acgtaggcct gataagacgc gccagcgtcg
     1141 catcaggcgc tgaatgccgg attgttaccc ggcatttgtc atttagcggt gatgtttgcc
     1201 tggaccatga ccgccgtgat gatcatgagg agctttctta tggtggcgcg gcggtggcgg
     1261 cggtccgtgt gggtgccagc gattgcctcg ccattcataa tgttgtttcc accagccgtg
     1321 gtcgcgccag tgacctccat cccagtaata gccacgatta tcacgattgc ctatctgtaa
     1381 ttttactgac gggactaacg taatttccgc agcctgtgct gccatgggag cgaccagaac
     1441 cagggaaagt gcgagtacga tagattgcat ctttttcaca ggttactcct tttatcattg
     1501 cccgttatgg gtcgatatcc ggagattacg cgatgagaac gacggttgtt attctccgca
     1561 atcctaatct ctaagttacc gcatttattg agaatatctc ctttttccct gtcgtttttc
     1621 tccataattt cttcataaaa aagccgggct gcataaaagc aaacccggcc tgattgagat
     1681 aatgaataga ttttactgat gattcatcat caatttacgc aacgcagcaa aatcggcggg
     1741 cagattatgt gaaagcaagg gtaaatcagc acgttctgcc agctcttttg gcagatccaa
     1801 cgtttcaccg agaatcgctt ccacgctctc tttaaatttc gccggatgcg cggtgccgag
     1861 gaacaagcca tattcgcctg gattcaactg gtcacgcagc gcacgatacg ctacggcagc
     1921 gtgcggctcc gaggtgtagc ccagttcttt taactcacgc attgtctgtt gcgtggtttc
     1981 atcatccacg gctgcataac ccagctcttt cagttgccag attttgcggc ggaacaactc
     2041 ttccacacgc ggccagttgt tcggctgact cacgtccatc gcgttggata acgtcgcctg
     2101 agtcgctttg ggtgaccact gaccgtcgtg caggaaacgt ggcacggtat cgttcacgtt
     2161 ggtcgcagca ataaaacgtt tcaccggcag accgagtgac ttcgccagca gacccgccgt
     2221 caaatcgccg aagtttccgc ttggcaccga gacaaccagc tggttgcgcg cttcctgcgg
     2281 cagctgcgca actgcttcaa agtagtagca aatctgcgcc agcaaacggc tgatgttaat
     2341 cgagttagct gagtttaacc ccagcgccac tttcagctct tcatcatcaa acgcctgctt
     2401 caccagcgcc tgacaggcat cgaaatcgcc gtcgatggca acagtttcga tattgccgcc
     2461 caatgtacag aacagttttt cttgcagtgg actgattttg cctcgtggat agaggataac
     2521 cactttcaca ttcggtaaac cgtagaaagc atgagccact gccgctccgg tatcaccgga
     2581 ggtcgcggtc agaatggtca ctggcttatc gcccgcaata tgggtcagca tttgtgccat
     2641 aaagcgaccg ccgaaatctt taaatgccag cgttggcccg tggaacaatt ccagacaacc
     2701 gacatcgctt tcaacattgg cgaccggagc cgggaaggca aacgccgcgc gcacgcgctc
     2761 ttccaggatt tcctgcggga tttcatcacc aataaacgcc gagaggatct tcgcactgcg
     2821 ggtgacaaaa tccagcttca gcatctcatc aatttcagtc aggctgaatt ccggcaggtc
     2881 gtgcggaaaa aacagcccct gatttttgcc caacccctgg gttacggctt gcgcaaagct
     2941 gacctgctca ttgtgatctt tcagattgta gagtttcatt tagttttcca gtactcgtgc
     3001 gcccgccgta tccagccggc aaatatgaac aaaaccttcc tgattttgca ggtagttctt
     3061 acccaaccag tcggcaacgc gctgggcggt atccggcttg tcacacagag cgaacaaggt
     3121 cgggccggag ccggagatac cgctcgctac cgcgccgatt tccgcaaccg cctgccgcgc
     3181 ctgccggaag ccaggcagta accgttcacg gtagggttca gcgataacat ctttcatcag
     3241 cttcgcggca agctcaggct gacgggaata gcaggcgtga atgaagccag ccagatgtcg
     3301 cccgtgcgca atgcaatcct ggcggcgata ctgcgccggt aaaatagccc tggcttctgc
     3361 cgttgagact ttaatccccg gatacgccag cacccacagc cactcatcaa accctggcac
     3421 ttgctggctg atgatgtcgt tttcttcgat catcaactgc ataccaccaa gaaaacacgg
     3481 tgccacgttg tcgtaatgaa tgctgccgga gattcgtcct tccagctcgc ccatcaaagc
     3541 cagcaaacga gtgtcattaa gcggcttgcc acagtgttca ttcatcgcca tcagcgccgc
     3601 gaccaccgaa caggcgctgg agcctaagcc cgaaccgatt ggcatattct tttccagagt
     3661 catcgccact ggaatttgct tgccaagctc ctggcaaaaa cgctcccagc actgataaac
     3721 gatattttcc cgtggttctg acggcagctt atcggcaaag cgtccgaggt tgttgagact
     3781 gaatgtctct gccgcctcaa ccgtgactac atctccgagc aatgcaccat caacaggtgt
     3841 caccgccgcc ccgagcacat caaacccgac gctcatattg gcactggaag ccggggcata
     3901 aactttaacc atgtcagact cctaacttcc atgagagggt acgtagcaga tcggcaaaga
     3961 caccggcagc tgtaacgtca ttgcccgcac catatccgcg cagcaccaac ggcagcggct
     4021 gataatagtg gctataaaag gccagggcgt tttcgccatt tttcactttg aacagcggat
     4081 cattaccatc cacttcggca atcttcacgc ggcaggcacc atcttcatca atattgccaa
     4141 catagcgcaa aacttttcct tcatcacggg ccttcgccac gcgcgcggca aagagatcgt
     4201 cgagctgtga cagattcgcc ataaaagcgg caacatcacc ctcagcgtta aactctgcgg
     4261 gcagcacagg ttcaatttca atatccgcca gctccagttc acgtcccgtt tcacgagcga
     4321 gaatcaatag cttacgcgct acatccatac cagaaagatc atctcgcgga tccggttcgg
     4381 tataacccat ttcccgcgcc agagtagtcg cctcggagaa actcatgcct tcgtctaact
     4441 tgccgaagat ataagaaagc gaacctgaaa gaatgccgga gaacttcatc aattcatcac
     4501 cagcattgag cagattttgc aggttctcaa taaccggtaa tccagcccca acgttggtgt
     4561 catagaggaa tttacgccgc gatttttccg ccgcatgacg caacagatgg tagtaatcca
     4621 tcgacgaggt gttggccttt ttgttcggcg tgacaacgtg gaaaccttcg cgcaagaagt
     4681 cggcatattg atccgccact gcctggctgg aggtgcagtc aacaatgacc gggttcagca
     4741 gatgatattc tttcacgagg cgaattaagc gcccgagatt aaacggctct ttggcttgcg
     4801 ccagttcttc ctgccagttt tccagattta ggccatgcac attggtgagc agtgccttcg
     4861 agttggcaac accgcagaca cgtaagtcga tatgtttatt cttcagccag ctttgttgac
     4921 gcttcagttg ctccagcagc gcaccgccaa cgccaccgac gccaatcaca aacacttcga
     4981 taacctgatc ggtattgaac agcatctgat gagtaacgcg cacgccagtg gtcgcatcat
     5041 cgttatttac cacgacagag attgagcgtt cagaagatcc ctgagcaatg gcgacaatgt
     5101 tgatattggc gcgggccagc gcggcaaaga atttcgccga gatcccacgc aaggtgcgca
     5161 taccatcacc taccaccgag ataatggcca gccgttccgt caccgccagc ggctccagta
     5221 agccttcttt cagttccagg tagaactctt cctgcattgc ccgttcagct cgcacacagt
     5281 cgctttgtgg aacgcagaaa ctgatactgt attcggaaga tgattgcgta atcagcacca
     5341 cggaaatacg ggcgcgtgac attgcagcaa agacgcgcgc cgccatgccg accattcctt
     5401 tcatccccgg accagaaacg ctgaacattg ccatattatt cagattggaa atgcctttga
     5461 ccggtaattc gtcttcatca cggctggcac caatgagcgt acctggtgct tgaggatttc
     5521 cggtattttt aatcaggcaa gggatctgga actgggcgat gggggtaatg gtgcgggggt
     5581 gaagaacttt agcgccgaag taggaaagct ccatcgcttc ctggtaggac atcgacttca
     5641 acaacctcgc atcgggcacc tgacgcgggt cgcaggtata gaccccgtca acgtccgtcc
     5701 aaatctcgca acaatcggcg cgtaaacagg cagccagcac cgccgcggag tagtcggaac
     5761 cgttacgtcc aagtaccacc agttcgcctt tttcattacc ggaggtgaaa cctgccatca
     5821 acaccatgtg atcagccgga atgcggcttg ccgcaatacg gcgggtggac tcagcaatat
     5881 cgacggtaga ttcgaggtaa tgccccactg ccagcagttt ttcgaccgga tcgataacgg
     5941 taacgttgtg accacgagct tctaacacgc cggccataat ggcgatcgac attttctcgc
     6001 cacggcaaat cagcgcagcg ttgatgctat ccgggcactg ccccaacaaa ctaatgccat
     6061 gcaggacatg ttttatttgg gcaaattcct gatcgacgaa agttttcaat tgcgccagcg
     6121 ggaaccccgg ctgggcggcg gcgagtcccg tcaaaagttc ggcaaaaata cgttcggcat
     6181 cgctgatatt gggtaaagca tcctggccgc taatggtttt ttcaatcatc gccaccaggt
     6241 ggttggtgat tttggcgggg gcagagagga cggtggccac ctgcccctgc ctggcattgc
     6301 tttccagaat atcggcaaca cgcagaaaac gttctgcatt tgccactgat gtaccgccga
     6361 acttcaacac tcgcatggtt gttacctcgt tacctttggt cgaaaaaaag cccgcactgt
     6421 caggtgcggg cttttttctg tgtttcctgt acgcgtcagc ccgcaccgtt acctgtggta
     6481 atggtgatgg tggtggtaat ggtggtgcta atgcgtttca tggatgttgt gtactctgta
     6541 atttttatct gtctgtgcgc tatgcctata ttggttaaag tatttagtga cctaagtcaa
     6601 taaaatttta atttactcac ggcaggtaac cagttcagaa actgctatca gacactcttt
     6661 ttttaatcca cacagagaca tattgcccgt tgcagtcaga atgaaaagct gaaaaatact
     6721 tactaaggcg ttttttattt ggtgatattt ttttcaatat catgcagcaa acggtgcaac
     6781 attgccgtgt ctcgttgctc taaaagcccc aggcgttgtt gtaaccagtc gaccagtttt
     6841 atgtcatctg ccactgccag agtcgtcagc aatgccatga ctcgttcgcg taaagcttgc
     6901 agttgatgtt ggtctgccgt tgtgtcactt ttcgtcggtt gttgtattaa tgttgctaat
     6961 tgatagcaat agaccatcac cgcctgcccc agattgagcg aaggataatc cgccaccatc
     7021 ggcacaccag taagaacgtc agccaacgct aactcttcgt tagtcagccc ggaatcttcg
     7081 cgaccaaaca ccagcgcggc atggctcatc catgaagatt tttcctctaa cagcagcacc
     7141 agttctactg gcgtggcgta gtaatgatat ttcgcccgac tgcgcgcagt ggtggcgaca
     7201 gtgaaatcga catcgtgtaa cgattcaacc agtgtcggga aaactttaat attatcaata
     7261 atatcaccag atccatgtgc gacccagcga gtggctggct ccagatgtgc ctgactatcg
     7321 acaatccgca gttcgctaaa acccatcgtt ttcattgccc gcgccgctgc cccaatattt
     7381 tctgctctgg cgggtgcgac cagaataatc gttatacgca tattgccact cttcttgacc
     7441 aaatgaccgc gaacagggtg atcactgtca acttattaca cggtgcgaat ttacaaatcc
     7501 ttaacgtaag tcgcagaaaa agccctttac ttatcttaag taaggctaaa ctatttcctg
     7561 actgtactaa cggttgaatt gttaaaaaat gctacatatc cttctgttta cttaggataa
     7621 ttttataaaa aataaatctc gacaattgga ttcaccacgt ttattagttg tatgatgcaa
     7681 ctagttggat tattaaaata atgtgacgaa agctagcatt tagatacgat gatttcatca
     7741 aactgttaac gtgctacaat tgaacttgat atatgtcaac gaagcgtagt tttattgggt
     7801 gtccggcccc tcttagcctg ttatgttgct gttaaaatgg ttaggatgac agccgttttt
     7861 gacactgtcg ggtcctgagg gaaagtaccc acgaccaagc taatgatgtt gttgacgttg
     7921 atggaaagtg catcaagaac gcaattacgt actttagtca tgttacgccg atcatgttaa
     7981 tttgcagcat gcatcaggca ggtcagggac ttttgtactt cctgtttcga tttagttggc
     8041 aatttaggta gcaaacatgc agaccccgca cattcttatc gttgaagacg agttggtaac
     8101 acgcaacacg ttgaaaagta ttttcgaagc ggaaggctat gatgttttcg aagcgacaga
     8161 tggcgcggaa atgcatcaga tcctctctga atatgacatc aacctggtga tcatggatat
     8221 caatctgccg ggtaagaacg gtcttctgtt agcgcgtgaa ctgcgcgagc aggcgaatgt
     8281 tgcgttgatg ttcctgactg gccgtgacaa cgaagtcgat aaaattctcg gcctcgaaat
     8341 cggtgcagat gactacatca ccaaaccgtt caacccgcgt gaactgacga ttcgtgcacg
     8401 caacctgctg tcccgtacca tgaatctggg tactgtcagc gaagaacgtc gtagcgttga
     8461 aagctacaag ttcaatggtt gggaactgga catcaacagc cgttcgttga tcggccctga
     8521 tggcgagcag tacaagctgc cgcgcagcga gttccgcgcc atgcttcact tctgtgaaaa
     8581 cccaggcaaa attcagtctc gtgctgaact gctgaagaaa atgaccggcc gtgagctgaa
     8641 accacacgac cgtactgtag acgtgacgat ccgccgtatt cgtaaacatt tcgaatctac
     8701 gccggatacg ccggaaatca tcgccaccat ccacggtgaa ggttatcgct tctgtggtga
     8761 tctggaagat taatcggctt taccaccgtc aaaaaaaacg gcgcttttta gcgccgtttt
     8821 tatttttcaa ccttatttcc agatacgtaa ttgatcgtcc attgtaactt ctttactggc
     8881 tttcattttc ggcagtgaaa acgcatacca gtcgatatta cgagtcacaa acatcatgcc
     8941 ggccagcgcc accaccagca cactggttcc caacaacagc gcgctatcgg cagagttgag
     9001 cagtccccac atcacaccat ccagcaacaa cagcgcgagg gtaaacaaca tgctgttgcg
     9061 ccagcctttc aataccgcct gcaaataaat accgttcatt aacgccccaa tcagactggc
     9121 gattatccat gccacggtaa aaccgatatg ttcagaaagc gccagcaaga gcaaataaaa
     9181 catcaccaat gaaagcccca ccagcaaata ttgcattggg tgtaaacgtt gcgcggtgag
     9241 cgtttcaaaa acaaagaacg ccataaaagt cagtgcaatc agcagaatgg cgtacttagt
     9301 cgcccagtcc gttaattggt attgatcggc tggcgtcgtc actgcgacgc taaacgcggg
     9361 gaagttttcc cagccggtat cattgcctga agcaaaacgc tcaccgagat tattagcaaa
     9421 ccagctgctt tgccattgcg cctgaaaacc tgactcgcta acttcccgtt tggctggtag
     9481 aaaatcacct aaaaaactgg gatgcggcca gttgctggtt aagggcattt cgctattacg
     9541 cccggcaggc accacagaaa gatcgccggt accgcttaaa ttcagtgcca tattcagctg
     9601 caggttctgc ttccgccagt ccccttcggg taaagggata tgcacgccct gcccgccttg
     9661 ctctaacccg gtaccgggtt caatggtcag tgccgttccg ttaacttcag gcgctttcac
     9721 cacaccaata ccacgcgcat ccccgacgct aatcacaata aagggcttgc ctaagatgat
     9781 atttggcgcg ttgagctcgc taagacgcga aacatcgaaa tcggctttta gcgttaaatc
     9841 actgtgccag acctgaccgg tataaatccc tatcttgcgt tcttccacgt tctgattgcc
     9901 atcaaccatc aatgactcag gtaaccaaaa atggataaaa cttcgtttcc gctccacggt
     9961 tttatcctct tcctgcaccg tataaagctc ggtcacagga atagcgataa gcggcccaac
    10021 gagtttttgc ggcccgctgg tactttggcg aatcgcatct tccacatcgc tacggtaatc
    10081 ggcacgttcg acaatcactt gccgaatcag cattatcgga atcaacaaca gcaatactgc
    10141 accaaacaag gtggtcattt tccagaacag gggggatttc aacatagtct tctcctttgc
    10201 aatggcagca ggatacgaag gctatgtggg aagaatttga agctatgtga agtgacggtg
    10261 aagtcgaagc gaggccagca cgccaccttc ctgcacgttg cgcagcgtga cttcgccgtt
    10321 aaacaaacgg gcgacctcac tgacgaacgc caaccccaga ccgctgcttt tttgcccatt
    10381 tgcacgcggc aaagagtaaa agcgttcaaa aatacgtgac agcgcgtagt caggaatacc
    10441 actaccggta tccagcacct taagggcgac gtgttcctga tccacttcgg cgcttagcgt
    10501 tatgcgaccg ctctcggggg taaaatcgat ggcgttatcc agtaaattcc ccagcgcctg
    10561 ctccagtaac gccggttcag cagcaacatt aacctcagtg ggcataacat gtagagtgat
    10621 gtttttttct gccaactgca cggtgcgcgc ttcgctgacg cggcgaaata atgccgccac
    10681 atcaacaaca gtcagaacga cttcctgacg attctccagt cttgcctggc gtagtaacgt
    10741 ttctaccaat gcctgcatac gcgcattttg cgtcagaatg ttgtcggtaa aacgagccac
    10801 cacttccggt ggcggacctt cgcgtaaaat ttccgccgcg ccacgaatcg ccgccagtgg
    10861 gctttttagc tcatgggtta acgcataaac atactgctca atatagtttt tcccttccag
    10921 cttcacgcgc atactttcca gcgcctgcgc aagcttacgc aactcgctac tgccgagttc
    10981 aggaagagga acgggcttat tgtcagtgac ggaatcggca tagcgagtga gccgggcaat
    11041 ggagcggttg atccaccaaa ccatgcctgc gccaatcacc agtgcaatcc ccagcaaaat
    11101 ggcgctggcc cataaaattc gccgctcgct acgcttaatg accggagcca tcgccgcgtt
    11161 cggtttgcct acgctcaaaa cgccaataag ccgcgagccg tccataatcg gcgcggcaac
    11221 atacatcacc gaactttcgg gatcggcagg attttgcaac gtgctgcgcg caccatactg
    11281 accacgcaac gttagccaga cgtcattcca gcgcgaataa tcctgcccaa cggctttatt
    11341 tgccgaatcg aacaatactt taccctgcga atcggtcata tagacatgat attcattgcg
    11401 cactttgtta atgccaccga tattggcgcg aaacggacga tgttgtagct gattaaacgc
    11461 ctgcgccagt tgcccatgcg ttgggtcccc agagagcaaa tcgggacgcg ccagctccgc
    11521 cagcaacgtt gcggtgtcga tcaacgtccc ctccgttgct cttcgcacgc ccggtttaac
    11581 ttctttgaca aaaatagcca gtacgaacca ggccgccacc gccaccagta aaaaatagcc
    11641 cagcagcaac cgcatgccga tacgcattac aggcccctca ggctatatcc catgccgcga
    11701 tgagtattaa tcggtgaaag atcggggttg atggcacgca gcttggcatg caacgtttta
    11761 atgtgggtat caacggtgcg atcgtaggtg tcctgcgcat cttcccacac gctatccatc
    11821 agttgctggc gggaccagac gcggcctggc gacttgagta acgtcttcag caataaaaac
    11881 tcataccgcg tcagcgttaa tggcgtgtca aaccagctga tctgcgccgc gggttcattc
    11941 aattcaaaat gaccaatacg gatgacggga gacggcgacg agaacttctt cacccgacgc
    12001 agtaaggtgc gcaccctggc gcacacttcg cggggtgaaa acggtttagc cacatagtcg
    12061 tcagcgccaa tttccagccc aagcaggcga tcgacctctt cacttcgggc cgtcaggaac
    12121 agtacaggta acgccggatg gagcgccagt aactggctgc acaattcaaa gccgctgata
    12181 tcaggcaaac caacgtcgag aatcatgacg tcgggaacca gctggcgagc tttatccagc
    12241 accggcaagc ctcgctcaaa gacctcgacg gcaaaacctt cctgctgcaa catgtagacc
    12301 agcgtgtcgg ctatcccttg ctcatcttcc actaaccaga ccgtttcccg ttgtataaat
    12361 cgcctctgtt attgccgcca cggcatgaca ggaaccgcgc taatcgcgtt tttcggcgaa
    12421 ccttctacaa ctttgtcgga gtaagccaga taagcgagcg cgttgcgttt ggcgtcataa
    12481 aagcgcacga cctgtaacga cttaaagacc agggacgtgc gttttttgaa tactacctcg
    12541 ccctgagctt tgccgttttt aatacgatcc gacagttcaa tcggcccgac ttgctgacaa
    12601 gaaatagccg catcggaggt atcttccgcc agacccaatc cccctttaat accacctgtt
    12661 ttcgcccggc tcacataaca ggtgacattt ttcacatcgg gatcgtcaaa ggcttccaca
    12721 acaattttgt gatccgggcc gatcatttta aatacggtgt cgaccgaacc aatctcttct
    12781 gcatgagcca atggccccag cattatcagg cttaaagaca ggatcaaatg cttgtatttc
    12841 atattgttac catttttatc tattacacga gcaattagtt cgtcacggta aataacatat
    12901 tgattaagaa caaaatctca attcttttat ttccgtcaag ccctaaaaca tactctacta
    12961 agaaaaaaac actgaatgct aaaacagcaa aaaatgctat tatccaatta cctgatgtca
    13021 ggtgctcgtt gttgaaagga tgaggatatt ttatggatca ggccggcatt attcgcgacc
    13081 ttttaatctg gctggaaggt catctggatc agcccctgtc gctcgacaat gtagcggcga
    13141 aagcaggtta ttccaagtgg cacttacaga gaatgtttaa agatgtcact ggccatgcta
    13201 ttggcgcgta tattcgtgct cgtcgtttgt cgaaatcggc ggtcgcacta cgcctgactg
    13261 cgcgtccgat tctggacatc gcgctgcaat accgcttcga ctctcaacag acatttaccc
    13321 gcgcattcaa gaagcagttt gcccagactc ctgcacttta ccgccgttct cctgaatgga
    13381 gcgcctttgg tattcgcccg ccgctacgtc tgggtgaatt cacaatgcca gagcacaaat
    13441 ttgtcaccct ggaagatacg ccgctgattg gtgttaccca gagctactcc tgttcgctgg
    13501 agcaaatctc tgatttccgc catgaaatgc gttatcagtt ctggcacgat tttctcggca
    13561 acgcgccgac cattccgcca gtgctctacg gcctgaatga aacgcgtccg agtcaggata
    13621 aagacgacga acaagaggta ttctatacca ccgcgttagc ccaggatcag gcagatggct
    13681 atgtactgac ggggcatccg gtgatgctgc agggcggcga atatgtgatg tttacctatg
    13741 aaggtctggg aaccggcgtg caggagttta tcctgacggt atacggaacg tgcatgccaa
    13801 tgctcaacct gacgcgccgt aaaggtcagg atattgagcg atactacccg gcagaagacg
    13861 ccaaagcggg agatcgccca attaatctgc gctgtgaact gctgattcct atccgccgtt
    13921 aacgctgcag ctcatctaat gcaggggcgt ccagatgcga gatgtcccct gcggtttcaa
    13981 cgacccagcc agacgccagc cacaggcttt cctgataatc cacgcgcgaa atagaacagt
    14041 tacgcagacg taagcggcgc gctgcccatg ctggtaatcc gagaatcgta ctcaccagac
    14101 atcccagtgc aataccatgg ctcaccagca gcggtcggct tccctgcggt aagtcccggc
    14161 aagattccag tgccgcattg acgcgatcgc tgagctcctg cattgactcg ccttcaggaa
    14221 tacgcccgtc aacggtgcca ttgaccagct gccgacgcca gttctcttct tcttcggtca
    14281 gagaatcgat atgtcttttt tccagcacac ccatgtttaa ttcacgcagg cgagaatcaa
    14341 agatgatgtc acagccgcag gcctgggcga tgatttccgc cgtacgccgg gtgcgtccta
    14401 aatcgctact gataatatgc gtaatgccaa gctctttggc acgggttgcc acctgcatcg
    14461 cctgttgctc acctttggcg gtcagcgggc tgtcagactg gccctgaata cgtcgctcgg
    14521 cgttccactg cgtttcaccg tggcggacta ggtatacctg taacatgctt tttttccgta
    14581 tactgctgta acgttaattt tctgagtaat gctgattatg caccaagttg tctgtgcgac
    14641 caccaatccc gctaaaattc aggccattct gcaggcattt cacgagatct tcggcgaagg
    14701 atcctgccat attgcatccg ttgccgtcga gagcggtgta ccggaacagc cctttggcag
    14761 tgaggaaacg cgcgctggcg cacgaaatcg ggtagcaaat gcccgccgtt tacttccaga
    14821 ggctgatttt tgggtggcga ttgaagctgg catcgatggt gacagcactt tcagttgggt
    14881 ggtgattgaa aacaccagcc agcgcggcga ggcgcgttcg gctactttgc cgttacctgc
    14941 ggtgattctg cagaaagtgc gtgaaggtga ggcgcttggt cccgtgatgt cgcgttatac
    15001 cggtattgat gagattggtc gtaaggaagg ggcgatcggc gtgtttaccg ccgggaaact
    15061 cactcgcgcc agcgtctatc accaggcggt gatcctggcg ctgagtccgt ttcacaatgc
    15121 cgtgtatcag gcattgtagg acggataagg cgttcacgcc gcatccggca ttcggtgcac
    15181 gatgcctgat gcgacgctgc cgcgtcttat caggcctaca aaatcaatcg cttttcagca
    15241 acacctcttc cagccactgg cgcagctcca cgggcgcggc tttcaggctg ttagatccac
    15301 gcgtaatcgt cgcgatgccc gcgccgagtt cattttttaa ctcacgctga ctcatttcgc
    15361 cacgcaacag ctcttcgaca atacgcacgc gagtccccaa cgcttcgcgc tcatctggcg
    15421 tcagcatcag gtttaacaac ggtaaatgga gatcgttttg gtaggcattc ttaagcaggt
    15481 cgacaaaacg taaccactcc tggtgacgct gttctgccat cgctgctgaa tagggtgatt
    15541 gttgggccat aatatgtcgc cattgttagc gggggaagca aaatgcctcc cccggttgta
    15601 ctcgctaaag agtacgatag catatcataa acgtgcggat cagtaacgac gtccccattc
    15661 cgtggcgctc atcaacgtcg gtttatcccc catgaaataa cggtagtaag cgtcataagc
    15721 cagcacgttc ttcacataac cgcgcgtctc ggagaacgga atactctcga caaatgccac
    15781 agcgtcgata cgcccggcgc tgttgccaag ccaggttcgc acccgtccag gtccggcgtt
    15841 ataagctgct gaggagaaaa tacggttatt gccaaactgc tgataaacat attgcaggta
    15901 actggtgcca atgttgatat tcgtttccgg atccagcaat tgcccagggc tgctataacc
    15961 aggaatagag aacatcttca ccgtatgggt cgctgtacca ggcataatct gcatcaggcc
    16021 gctggcccct accggtgatt tcactttcgg attccaggcg ctctcctgac gggcaatcgc
    16081 catcgcatag ctttgcggga tctctttccc actggtgtaa cgtttgaaaa gatcgttgta
    16141 agccagcggg aatcgctctt ccagatgatc ccaaagcttc ccggcgatcg ttgcctgaac
    16201 gctaagatcc caccattggt tattgaaagc ataccgagcc agttgagcct gctctgtttt
    16261 tgacttgctc ttcaccagat tggcccactc gctacgcgcg gtgttatcga gattccagta
    16321 catcaactcg cgcacgcgcg ccatctccgg cccctgagtc agggcgctgt caacattctg
    16381 cggcgcttta tcaatcttca gctcatactc ttctccgatg cgttgtgctg caaccatcgg
    16441 gtagaaacca cgctgttgca tgagttgatg caaaatctct ttcgcttcag cttcacgtcc
    16501 gcgttccagc aataaatccg cctgccagta acgccattca tctttctctt ttgcttccat
    16561 cggcagacgc gccagccagg tattcaggcc gcgacgatcg ccggtgccaa gtgccattcg
    16621 cacacggcgt tcaatcaacg acgtggattg cgagcgcata atggcgtcat cgcgccattt
    16681 cgcctgctcg tcggtgacat cgttgcccat caaacgccag gcgacgatat cgcgcagctc
    16741 ctgaatctga tcttcattaa gctgctgcgc ctgggcaagc gacgggatca tcagccgtgc
    16801 attttcagca tcctgccgcg ccacactggc aaacgccacc gctgccattt gacgggtaaa
    16861 atcggtcgct ccggttgtac gcgcgaaggt cagtaccgta ttagggttgt tcgccagtga
    16921 aatgattgcc gaggcgatag tctggtaatc ggcaggcatc tgccctgcca gcactgttac
    16981 caggccagtg ttacccgctt tcatcgccag acggatacgc tctaaatacg ccagcggatc
    17041 ttgtttacct gacgcacgcc agacgctaaa taacttgtca caggcgttag gctggctctt
    17101 gccggttagc cacagctctt tcgccccttg ccaggcttct tcactctgcc cggtgttcca
    17161 tttcgcatag tagtaattac attgcgcttc ggtagttccg ggcttttccg ggctaaaggc
    17221 taacaagcca cgccagtctt cacgccgcgc cagttcattg acgaaacgag attgcagcgt
    17281 gcgagcggga ggaagcgtgg ggttagcgcg aacaaagtta gtgaccgtca ccgccggttg
    17341 attcatcaga tcatcggtga tctggcggta ttccaggtag ggataaagcg gataatcctt
    17401 cagtccaggc atcatttgtt cgaccacatc catttgtcga ttatcccagg cctgcttgat
    17461 ttgcgcgtaa cggctacgct gctcatccag tgagtcggct cgcgccacgc tgctgaccgt
    17521 cagcagacag acaccggcag ccaacagccg ccaggtaact tgtttggctt tttccacaag
    17581 cgcatcctct aagtgtaaat acatcaatgc agcgtcatgc cgcgtaatag atttatgcta
    17641 accagtcatt gccgtttacg ccacgttacg gacacttttt tacttttact gcgaaggtga
    17701 tcgaccaact gggattaggc ggggaaaaag gctacacttc gcctttgaaa actcatcatc
    17761 acgataaaca tagaggcgaa gtccaacgtg gctcaattcg tttataccat gcatcgtgtc
    17821 ggcaaagttg ttccgccgaa acgtcatatt ttgaaaaaca tctctctgag tttcttccct
    17881 ggggcaaaaa ttggtgtcct gggtctgaac ggcgcgggta agtctaccct gctgcgcatt
    17941 atggcaggca ttgataaaga catcgaaggt gaagcgcgtc cgcagccaga catcaagatt
    18001 ggttacctgc cgcaggaacc gcagctgaac ccggaacaca ccgtgcgtga gtccattgaa
    18061 gaagcggttt ctgaagtggt taacgccctg aaacgcctgg atgaagtgta tgcgctgtac
    18121 gccgatccgg atgccgattt tgacaagctg gccgctgaac aaggccgtct ggaagagatc
    18181 attcaggctc acgacggtca taacctgaac gtacagctgg agcgtgcggc ggatgcgcta
    18241 cgtctgccgg actgggacgc gaaaatcgct aacctctccg gtggtgagcg tcgtcgcgta
    18301 gcgttgtgcc gcctgctgct ggaaaaacca gacatgctgc tgctcgacga accgaccaac
    18361 cacctggatg ccgaatccgt ggcctggctg gaacgcttcc tgcacgactt cgagggcacc
    18421 gtggtggcga ttacccacga ccgttacttc ctcgataacg ttgcaggctg gatcctcgaa
    18481 cttgaccgcg gtgaaggtat tccgtgggaa ggcaactact cctcctggct agagcagaaa
    18541 gatcagcgcc tggcgcagga agcttcacaa gaagcggcgc gtcgtaagtc gatcgagaaa
    18601 gagctggagt gggtacgtca gggaactaaa ggccgccagt cgaaaggtaa agcacgtctg
    18661 gcacgctttg aagagctgaa cagcaccgaa tatcagaaac gtaacgaaac caacgaactg
    18721 tttattccac ctggaccgcg tctgggcgat aaagtgctgg aagtcagcaa cctgcgtaaa
    18781 tcctacggtg atcgcctgct gattgatgac ctgagcttct cgatcccgaa aggggcaatc
    18841 gtcgggatca tcggtccgaa cggcgcgggt aaatcgaccc tgttccgtat gatctctggt
    18901 caggaacagc cggacagcgg caccatcact ttaggtgaaa cggtgaaact ggcatcggtt
    18961 gatcagttcc gtgactcaat ggataacagc aaaaccgttt gggaagaagt ttccggcggg
    19021 ctggatatta tgaagatcgg caacaccgag atgccaagcc gcgcctacgt tggccgcttt
    19081 aactttaaag gggttgatca gggtaaacgc gttggtgaac tttccggtgg tgagcgcggt
    19141 cgtctgcatc tggcgaagct gctgcaggtt ggcggcaaca tgctgctgct cgacgaacca
    19201 accaacgacc tggatatcga aaccctgcgc gcgctggaaa acgccctgct ggagttcccg
    19261 ggctgtgcga tggttatctc gcacgaccgt tggttcctcg accgtatcgc cacgcacatc
    19321 ctggactacc aggatgaagg taaagttgag ttcttcgaag gtaactttac tgagtacgaa
    19381 gagtacaaga aacgcacgct gggcgcagac gcactggagc cgaagcgtat caagtacaag
    19441 cgtattgcga agtaatgcgt aaaatgccgg atgcggcgtg aacgccttat ccggcctaca
    19501 aaccaagaaa tttcaatacg ttgtgaatct ccaggcctga taagacgcgg taagcgtcgc
    19561 atcaggcatt gagcaccgaa tgccggatac ggcgtgaacg ccttttccgg cctacaatag
    19621 ccttagtatc cgcaatttcg tagcgggtca cccgtgctga ttcttttaaa ccttgtttaa
    19681 tctcgacagc ctcttccaga gaggttttca actcatcaaa aaacttattt tatacctcgc
    19741 ctttgagccg tttcatcgcg gttatctctg ctcccccatc atctcccgca ccagctccac
    19801 gcagcgcagg aaacggctgt cataatcgtc ctcttcaacc cgcacgaatt cgatattgtt
    19861 ctcttccagc atctccacca gcaagttctg gaactctttg cgatccaccg aactgccgag
    19921 gctgcgtaaa ccatccgcca cccacggcgt gttgttctcc agcaggatca ccagatcgaa
    19981 acggtattca tcaatcaacg cctgtacgaa cggatgctca cgcccttcgt actttttgca
    20041 gaacgcctgg gtagtgacaa aatcagtgtc aataaacgcc actttattgg catatttcac
    20101 tgcaaaatca atgtattgtg cgtggcccag cgcgatttta tcgtaatcgg aatactgcaa
    20161 tgcgatctca tcaccgccga ggtgtgaaaa gacataatcg cgaccatatt cccacgcact
    20221 ggtggtattg aagatattgg caagtttgtt taccagggtg gatttaccgc tcgactcgcc
    20281 accaaggatc gccacggtac gtacaaagaa cggcttcact tccgtaggaa tatattccca
    20341 gtagcggaac gggttttcgc ggatctgcgc accgctgata ctcataaagg tacgtttcgg
    20401 atcgaccagc accgtatcga tccccagatg ttccatatac tgtggcgcat cggcttcttc
    20461 cgaggtgtag atcaggtccg gctgaatccc cttttcagcc ataaactttt tgatgccgtt
    20521 gctccacaca tcccagccgt gcggatacgg ctccatgccc tcctcgttga aagcatgaat
    20581 gcgaatattt ttctgatatt taaaagtttg taataaccag cgcagacgat ccggcaccgt
    20641 gggctgctgc gacatggcgc tgtcttcaaa caacgcgcgg tcgcgggtat cgtcaaaacc
    20701 cataatgata tgcagctcgt caacctggct acaggcgcgc tggataaggt agatatgtcc
    20761 ggtatgcagc gggtagaact taccgaatac gacaccaatg gttttcttct gtcggggaaa
    20821 ttcaagcccc aaaaaacggt gcaacgcctc cagcttttgc gcgctggggc ttttaatttt
    20881 ggcattcagt aactggctta aatacccttt ggtcataccg ctggcatcag ctacctgctg
    20941 tagcgtgcag ccctgttgct tgatggcagt tttcaggtaa tcaaatgacg acatatctcc
    21001 ctccgtatct ctcattataa gtcgtcgaac acgctaagcg cgtcggagag ttttttaacg
    21061 ccaaaaatct gcatcccttc cggcgctttt ttcggtacgt tagccgccgg aacaatcgcc
    21121 cggcgaaaac cgtgtttcgc cgcttctgaa attcgttcct gaccgctggg caccgggcgg
    21181 atctcccctg ccagcccgac ttcaccaaac accaccagat cctgtggcag cggtctgtca
    21241 cgcaggctgg aaaccatcgc cagcagtaac gctaaatcgg cactggtttc ggttaccttc
    21301 acgccgccga ccacgttcac aaatacatcc tgatcggcca tttgcagacc accgtgacgg
    21361 tgcaagacag ccagcaggat tgccagacgg ttttgttcca gccccactgc cacgcggcgc
    21421 ggattcgcca tcatcgagtg atcgaccagc gcctgaatct ccaccagcaa cggacgcgtt
    21481 ccttcccaca ccaccatcac ggagctgccg gaggtcactt catctccgcg acttaagaaa
    21541 attgccgaag ggttgctgac ttcacgcagc ccctgttcgg tcatcgcgaa gacgcccagc
    21601 tcattcaccg cgccgaagcg gtttttatgg ctgcgcaagg tgcgaaaacg ggagtcggca
    21661 tcaccatcca gaagcaccga acagtcgata cagtgttcca gcactttagg gccagccagc
    21721 gagccatctt tggttacgtg cccaaccatg acaatcgcca caccgcgcgt tttggcgaag
    21781 cgcgtcagat aagccgccgt ttcacgcacc tgcgccacgc taccaggcga tgattgtaca
    21841 tccgccatat gcatcacctg gatcgagtca attaccatca gtttcggttg ctcttcttcg
    21901 gcaatcaggc agatctgttc gatgctggtt tccgacaaca tattgagatt gtcagtcggc
    21961 aggccaaggc gatgagcgcg cattgccacc tgttgcagcg actcttcgcc agtgacatac
    22021 agcgttttca tctgctgggc cagtttgcac agcgtttgca gtagcagcgt ggatttcccc
    22081 gcacctgggt taccgccaat cagaatggcg cttcccggca ccacgccgcc gcctagtacg
    22141 cggtcgaact ctttaaatcc ggtggaaaaa cgcggcagct cttcaaggct gatatcggag
    22201 agtttctgga cttttgccac cccggcgcta ccggcatagc cgctgagacg ctcgttacgc
    22261 gccaccgttg gcgacgcagc aagacgcacc tcggtgatgg tgttccaggc atgacaggca
    22321 ctgcactgcc cctgccagcg cggataatcg gccccgcatt cattacaaac aaaggcgcgt
    22381 tttggagctt ttgccacggt gtacctcgtt aatgctgtgc cgcccgcagg atggcgggcg
    22441 agcaattact tctgattcag gctgccagag aggatgcaga atacccccat caggtcagcg
    22501 tgacggatgg tgacttccgc cttttcattc acttttggct tggcatggta ggcaatcccc
    22561 agccctgctg ctttgatcat cggcaggtca ttagctccat cgccaatcgc cacggtctgc
    22621 gccagcggga tttcatactc ctgcgcgagg cgagtcaaag ttttcgcttt gtactgcgcg
    22681 tctacgatgt cgccgatcac attgccggta aatttaccgt ccatgatctc cagttcattg
    22741 gctaccacgg cggtcaggcg cagcttgtcg cgcagatatt cagcaaagaa agtaaagccg
    22801 ccggaggcaa tcgccacttt ccagcccagc gtttccagct tgagcaccag ttgcgttaag
    22861 cctggcatca gcggcagatt ttcacgcacc tgttgcagaa tattggcgtc agcgcctttc
    22921 agcgtcgcca cacggctgcg caggctggcg gtaaaatcga gttcgccgcg catcgcccgt
    22981 tcggttactt ccgccaccat ctcgcccgtt ccggccagtt tggcaatttc atcaatacat
    23041 tcaatctgga tggcggtgga atccatgtcc atcaccagca aacccggcgt gcgcaggtgc
    23101 gggattttcc ccagcggggc gacatccagt tgcgcttcat gggccaggcg cgtagcccgt
    23161 gcggtgagtg aacctgccag acgaatcacc tgataatctt ccacgcacca ggcggcaaca
    23221 atcaccatcg ccgcacccag tttgctctgg tattgggtca gacgctgttt atccagccca
    23281 cgaccataca gcagccagcc gctacgacct gcgtggtaat ccagtggcat cacttcatca
    23341 ccacttaatg aaagaggcag accaggccat aaagagacat cttcaggcag gtcgcaccag
    23401 gtaatgttag gcattaagga tcctgtaaaa tcgttcgaag cagggaaaat aacgcatgag
    23461 gctaccttgt atccattgct tctggcaaca ttaagtctca aattttcaaa gggtggaaga
    23521 tggctcgcac aaaactgaaa ttccggctgc atcgggcagt gattgtcctg ttctgtcttg
    23581 ccttgttagt ggcgctgatg cagggagcgt catggtttag tcaaaaccac cagcgacaac
    23641 gtaatccaca gctggaagaa ctggcccgca ccctggcgcg tcaggtaacg ctgaacgttg
    23701 caccgctgat gcgtaccgac tcaccggatg aaaaacgcat tcaggcgatc ctcgatcagt
    23761 taacggatga aagccgtatc ctcgacgcgg gtgtgtatga cgaacaaggc gatcttatcg
    23821 cacgttctgg cgaaagcgtc gaagtgcgcg accggctggc gctcgacggt aaaaaagcgg
    23881 gcggctattt taaccagcag attgtcgaac caattgccgg gaaaaacgga ccgctcggct
    23941 atctgcgcct gacgctcgac acccatacgc tcgccaccga agcccaacag gtggataaca
    24001 ccactaacat tttacgcctg atgttgctgc tttcactggc aatcggcgta gtgctgaccc
    24061 gcacgctgct acagggtaaa cgtacccgct ggcagcaatc gcccttcctg ttaaccgcca
    24121 gcaaaccggt gccggaagag gaagaaagcg agaaaaaaga gtgacccatt actacaagaa
    24181 aggaaatcgt tatgtccaca ttacgcctgc tcatctctga ctcttacgac ccgtggttta
    24241 acctggcggt ggaggagtgt atttttcgcc agatgcccgc cacgcagcgc gttctgtttc
    24301 tctggcgcaa tgccgacacg gtagtgattg gtcgcgcgca gaacccgtgg aaagagtgta
    24361 atacccggcg gatggaagaa gataacgtcc gcctggcacg gcgcagtagc ggtggcggcg
    24421 cggtgttcca cgatctcggc aatacctgct ttacctttat ggctggcaag ccggagtacg
    24481 ataaaaccat ctccacgtcg attgtgctca atgcgctgaa cgcgcttggc gtcagcgccg
    24541 aagcgtccgg gcgtaacgat ctggtggtga aaaccgccga aggcgaccgc aaagtctcag
    24601 gatcggccta tcgcgaaacc aaagatcgtg gcttccacca cggcaccttg ctgctcaatg
    24661 ccgaccttag ccgcctggca aactatctca atccggataa aaagaaactg gcggcgaaag
    24721 gcattacctc agtgcgttcc cgcgtgacca acctcaccga gctgctgccg gggatcaccc
    24781 atgagcaggt ttgcgaggcc ataaccaagg cctttttcgc ccattatggc gagcgtgtag
    24841 aagcggaaat catctccccg gacaaaacgc cagacttgcc aaacttcgcc gaaatctttg
    24901 cccgtcagag tagctgggaa tggaacttcg gtcaggctcc ggcattctcg catctgctgg
    24961 atgaacgctt tagctggggc ggcgtggaac tgcatttcga cgttgaaaaa ggccatatca
    25021 cccgcgccca ggtgtttacc gacagcctca accccgcgcc gctggaagcc ctcgccgggc
    25081 gactgcaagg ctgcctgtac cgcgcggata tgctgcaaca agagtgcgaa gcgctgttgg
    25141 ttgacttccc ggaccaggaa aaagagctac gggagttgtc gacgtggata gcgggggcgg
    25201 taaggtaact cgctgattaa aataccaaag gaagggaata tgatcgatct ggaaaaccag
    25261 gagcgggaga ttatcaattt aatgttaagc cagcgtattt cctggctagc cgccgtgcgt
    25321 atcaggcata aattatcgct tgctgaagta agtaaaatgt taggtatctc gattaattct
    25381 ctaaaataga tcgaaaagac ggaacggttt agctcaaaca tcaaaagcaa aatggcagaa
    25441 atttatggtt gtccaccaga gttgctgata tgcccttcct ggatgacggc tgaacataaa
    25501 tgaatagatt
//
LOCUS       DAJVUJ010000044        25232 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.044, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000044 DAJVUJ010000000
VERSION     DAJVUJ010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25232
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.044"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(<1..393)
                     /locus_tag="OQQ48_001669"
     CDS             complement(<1..393)
                     /locus_tag="OQQ48_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCS1952479.1"
                     /translation="MDTTLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFY
                     QVCHPDGLADALWGTIVGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHSWAFL
                     PRLVFLAASFACGAVVIGLLMRGKESLKN"
     gene            772..1083
                     /gene="rpsJ"
                     /locus_tag="OQQ48_001670"
     CDS             772..1083
                     /gene="rpsJ"
                     /locus_tag="OQQ48_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCS1952480.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            1116..1745
                     /gene="rplC"
                     /locus_tag="OQQ48_001671"
     CDS             1116..1745
                     /gene="rplC"
                     /locus_tag="OQQ48_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCS1952481.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            1756..2361
                     /gene="rplD"
                     /locus_tag="OQQ48_001672"
     CDS             1756..2361
                     /gene="rplD"
                     /locus_tag="OQQ48_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCS1952482.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            2358..2660
                     /gene="rplW"
                     /locus_tag="OQQ48_001673"
     CDS             2358..2660
                     /gene="rplW"
                     /locus_tag="OQQ48_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCS1952483.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            2678..3499
                     /gene="rplB"
                     /locus_tag="OQQ48_001674"
     CDS             2678..3499
                     /gene="rplB"
                     /locus_tag="OQQ48_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCS1952484.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            3516..3794
                     /gene="rpsS"
                     /locus_tag="OQQ48_001675"
     CDS             3516..3794
                     /gene="rpsS"
                     /locus_tag="OQQ48_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCS1952485.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            3809..4141
                     /gene="rplV"
                     /locus_tag="OQQ48_001676"
     CDS             3809..4141
                     /gene="rplV"
                     /locus_tag="OQQ48_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCS1952486.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            4159..4860
                     /gene="rpsC"
                     /locus_tag="OQQ48_001677"
     CDS             4159..4860
                     /gene="rpsC"
                     /locus_tag="OQQ48_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCS1952487.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            4873..5283
                     /gene="rplP"
                     /locus_tag="OQQ48_001678"
     CDS             4873..5283
                     /gene="rplP"
                     /locus_tag="OQQ48_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCS1952488.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            5283..5474
                     /gene="rpmC"
                     /locus_tag="OQQ48_001679"
     CDS             5283..5474
                     /gene="rpmC"
                     /locus_tag="OQQ48_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCS1952489.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            5474..5728
                     /gene="rpsQ"
                     /locus_tag="OQQ48_001680"
     CDS             5474..5728
                     /gene="rpsQ"
                     /locus_tag="OQQ48_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCS1952490.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            5893..6264
                     /gene="rplN"
                     /locus_tag="OQQ48_001681"
     CDS             5893..6264
                     /gene="rplN"
                     /locus_tag="OQQ48_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCS1952491.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            6275..6589
                     /gene="rplX"
                     /locus_tag="OQQ48_001682"
     CDS             6275..6589
                     /gene="rplX"
                     /locus_tag="OQQ48_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCS1952492.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            6604..7143
                     /gene="rplE"
                     /locus_tag="OQQ48_001683"
     CDS             6604..7143
                     /gene="rplE"
                     /locus_tag="OQQ48_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCS1952493.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            7158..7463
                     /gene="rpsN"
                     /locus_tag="OQQ48_001684"
     CDS             7158..7463
                     /gene="rpsN"
                     /locus_tag="OQQ48_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCS1952494.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            7497..7889
                     /gene="rpsH"
                     /locus_tag="OQQ48_001685"
     CDS             7497..7889
                     /gene="rpsH"
                     /locus_tag="OQQ48_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCS1952495.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            7902..8435
                     /gene="rplF"
                     /locus_tag="OQQ48_001686"
     CDS             7902..8435
                     /gene="rplF"
                     /locus_tag="OQQ48_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCS1952496.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRIKEAKKK"
     gene            8445..8798
                     /gene="rplR"
                     /locus_tag="OQQ48_001687"
     CDS             8445..8798
                     /gene="rplR"
                     /locus_tag="OQQ48_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCS1952497.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            8813..9316
                     /gene="rpsE"
                     /locus_tag="OQQ48_001688"
     CDS             8813..9316
                     /gene="rpsE"
                     /locus_tag="OQQ48_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCS1952498.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            9320..9499
                     /gene="rpmD"
                     /locus_tag="OQQ48_001689"
     CDS             9320..9499
                     /gene="rpmD"
                     /locus_tag="OQQ48_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCS1952499.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            9503..9937
                     /gene="rplO"
                     /locus_tag="OQQ48_001690"
     CDS             9503..9937
                     /gene="rplO"
                     /locus_tag="OQQ48_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCS1952500.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            9945..11276
                     /gene="secY"
                     /locus_tag="OQQ48_001691"
     CDS             9945..11276
                     /gene="secY"
                     /locus_tag="OQQ48_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCS1952501.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            11308..11424
                     /gene="rpmJ"
                     /locus_tag="OQQ48_001692"
     CDS             11308..11424
                     /gene="rpmJ"
                     /locus_tag="OQQ48_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCS1952502.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            11571..11927
                     /gene="rpsM"
                     /locus_tag="OQQ48_001693"
     CDS             11571..11927
                     /gene="rpsM"
                     /locus_tag="OQQ48_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCS1952503.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            11944..12333
                     /gene="rpsK"
                     /locus_tag="OQQ48_001694"
     CDS             11944..12333
                     /gene="rpsK"
                     /locus_tag="OQQ48_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCS1952504.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            12367..12987
                     /gene="rpsD"
                     /locus_tag="OQQ48_001695"
     CDS             12367..12987
                     /gene="rpsD"
                     /locus_tag="OQQ48_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCS1952505.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            13013..14002
                     /gene="rpoA"
                     /locus_tag="OQQ48_001696"
     CDS             13013..14002
                     /gene="rpoA"
                     /locus_tag="OQQ48_001696"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCS1952506.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            14043..14426
                     /gene="rplQ"
                     /locus_tag="OQQ48_001697"
     CDS             14043..14426
                     /gene="rplQ"
                     /locus_tag="OQQ48_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCS1952507.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            14533..14901
                     /locus_tag="OQQ48_001698"
     CDS             14533..14901
                     /locus_tag="OQQ48_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HCS1952508.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILRGIRHDDPQYQEVSRRLSLLELKLRQAGF
                     STDFLRGDYADKLLDKINDN"
     gene            14912..15337
                     /gene="zntR"
                     /locus_tag="OQQ48_001699"
     CDS             14912..15337
                     /gene="zntR"
                     /locus_tag="OQQ48_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HCS1952509.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            15393..15611
                     /gene="arfA"
                     /locus_tag="OQQ48_001700"
     CDS             15393..15611
                     /gene="arfA"
                     /locus_tag="OQQ48_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HCS1952510.1"
                     /translation="MSRYQHTKGQIRDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(15608..16018)
                     /gene="mscL"
                     /locus_tag="OQQ48_001701"
     CDS             complement(15608..16018)
                     /gene="mscL"
                     /locus_tag="OQQ48_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HCS1952511.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLAEIRDLLKEQNNRS"
     gene            complement(16148..17524)
                     /gene="trkA"
                     /locus_tag="OQQ48_001702"
     CDS             complement(16148..17524)
                     /gene="trkA"
                     /locus_tag="OQQ48_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HCS1952512.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(17546..18835)
                     /gene="rsmB"
                     /locus_tag="OQQ48_001703"
     CDS             complement(17546..18835)
                     /gene="rsmB"
                     /locus_tag="OQQ48_001703"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCS1952513.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAKFNASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(18881..19828)
                     /gene="fmt"
                     /locus_tag="OQQ48_001704"
     CDS             complement(18881..19828)
                     /gene="fmt"
                     /locus_tag="OQQ48_001704"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCS1952514.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERYIRAFNPWPMSWLEIERQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLA"
     gene            complement(19843..20352)
                     /gene="def"
                     /locus_tag="OQQ48_001705"
     CDS             complement(19843..20352)
                     /gene="def"
                     /locus_tag="OQQ48_001705"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCS1952515.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            20482..21606
                     /gene="dprA"
                     /locus_tag="OQQ48_001706"
     CDS             20482..21606
                     /gene="dprA"
                     /locus_tag="OQQ48_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCS1952516.1"
                     /translation="MVDTDIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLATTDYPGALFVEGEL
                     YALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLATSLLEHGGALVSEFPLDVSPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            21578..22051
                     /locus_tag="OQQ48_001707"
     CDS             21578..22051
                     /locus_tag="OQQ48_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HCS1952517.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLTEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLEI
                     REMVIERVLALDTAEFDLEDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            22080..22622
                     /locus_tag="OQQ48_001708"
     CDS             22080..22622
                     /locus_tag="OQQ48_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HCS1952518.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            22627..23199
                     /gene="tsaC"
                     /locus_tag="OQQ48_001709"
     CDS             22627..23199
                     /gene="tsaC"
                     /locus_tag="OQQ48_001709"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HCS1952519.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVLGETGGRLNPSEIRDALTGELFRQG"
     gene            23204..24022
                     /gene="aroE"
                     /locus_tag="OQQ48_001710"
     CDS             23204..24022
                     /gene="aroE"
                     /locus_tag="OQQ48_001710"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCS1952520.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFSAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLQGDNTD
                     GIGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHLGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            24019..24276
                     /locus_tag="OQQ48_001711"
     CDS             24019..24276
                     /locus_tag="OQQ48_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HCS1952521.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(24252..24806)
                     /locus_tag="OQQ48_001712"
     CDS             complement(24252..24806)
                     /locus_tag="OQQ48_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HCS1952522.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGGVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         6454 a         5994 c         6564 g         6220 t
ORIGIN      
        1 gttttttaat gattcttttc ctctcatgag cagaccaata actacggctc cgcaagcgaa
       61 tgaggcagca aggaaaacca gtcgtggtaa gaaggcccat gaatgccagg cgccaagggc
      121 ggcgagaaac ttcacatcac catagcctaa tccttcttta tgtcgcagta tgcgataccc
      181 ccagtaaatg acagcgaatg tgccgtaacc aacaattgtc ccccataacg catcggctaa
      241 accatcagga tgacaaactt gatagaacaa aagacctgac cagagtaacg ggcacgtaaa
      301 tctgtcaggt aacaagccat gctttgcatc ccagaaaaaa agtagggctg ataaacaagc
      361 gtacaaaatc agaaagggaa gggtagtgtc cataaaacat cctctgtaat tggagtatca
      421 ccatcgaata actccggatg ctcagcaaca gagagagcgc tgatttgtta acctgattcc
      481 ggtgaaaaac gctatcttca caaccttaca aatttcgtgg aatgcagctc gcaatctgcg
      541 cgttgcgatt aatttctacc cgatgcttgc gtcaggctca gatttgcgta taatgcgcgg
      601 gcttgtcgta gttgacagca ggttcaatct gaacctcagt tagccgaatt tggctaccta
      661 acaatgctcc caatcgggga gctacgtaag aacggttaca ctctcccatc aatcgtaatg
      721 ggtctgagga gtaatcattt tcgtttataa aataattgga gctctggtct catgcagaac
      781 caaagaatcc gtatccgcct gaaagcgttt gatcatcgtc tgatcgatca agcaaccgcg
      841 gaaatcgtcg agactgccaa gcgcactggt gcgcaggtcc gtggtccgat cccgctgccg
      901 acacgcaaag agcgcttcac tgttctgatc tccccgcacg tcaacaaaga cgcgcgcgat
      961 cagtacgaaa tccgtactca cttgcgtctg gttgacatcg ttgagccaac cgagaaaacc
     1021 gttgatgctc tgatgcgtct ggatctggct gccggtgtag acgtgcagat cagcctgggt
     1081 taatcaggtc attgagcgat tgagaggttg aaacaatgat tggtttagtc ggtaaaaaag
     1141 tgggtatgac ccgtatcttc acagaagacg gcgtttctat cccagtaacc gtaatcgaag
     1201 ttgaagcaaa ccgcgttact caggttaaag acctggctaa cgatggctac cgtgctattc
     1261 aggtgaccac cggtgctaaa aaagctaacc gtgtgaccaa gcctgaagct ggccacttcg
     1321 ctaaagctgg cgtagaagct ggccgtggtc tgtgggaatt ccgcctggct gaaggcgaag
     1381 agttcactgt aggtcagagc attagcgttg aactgtttgc tgacgttaaa aaagttgacg
     1441 taactggcac ctctaaaggt aaaggtttcg caggtaccgt taagcgctgg aacttccgta
     1501 cccaggacgc tactcacggt aactccttgt ctcaccgcgt tccgggttct atcggtcaga
     1561 accagactcc gggcaaagtg ttcaaaggca agaaaatggc aggtcagatg ggtaacgaac
     1621 gtgtaaccgt tcagagcctt gacgtagtac gcgttgacgc tgagcgcaac ctgctgctgg
     1681 ttaaaggtgc tgtcccgggt gcaaccggta gcgacctgat cgttaaacca gctgtgaagg
     1741 cgtaaggaga tagcaatgga attagtattg aaagacgcgc agagcgcgct gactgtttcc
     1801 gaaactacct tcggtcgtga tttcaacgaa gcgctggttc accaggttgt tgttgcttat
     1861 gcagctggtg ctcgtcaggg tactcgtgct cagaagactc gtgctgaagt aactggttcc
     1921 ggtaaaaaac cgtggcgcca gaaaggcacc ggccgtgcgc gttctggttc tatcaagagc
     1981 ccgatctggc gttctggtgg cgtgaccttt gctgctcgtc cgcaggacca cagtcaaaaa
     2041 gttaacaaga agatgtaccg cggcgcgctg aaaagcatcc tgtccgaact ggtacgtcag
     2101 gatcgtctga tcgttgtcga gaagttctct gtagaagcgc cgaaaactaa gctgctggca
     2161 cagaaactga aagacatggc tctggaagat gtgctgatca tcaccggtga gctggacgaa
     2221 aacctgttcc tggctgcgcg caacctgcac aaggttgacg tacgcgatgc aactggtatc
     2281 gacccggtta gcctgatcgc cttcgacaaa gtcgtaatga ctgctgatgc tgttaagcaa
     2341 gttgaggaga tgctggcatg attcgtgaag aacgtctgct gaaggtgctg cgtgcaccgc
     2401 acgtttctga aaaagcgtct actgcgatgg aaaaatccaa caccatcgta ctcaaagttg
     2461 ctaaagacgc gaccaaagca gaaatcaaag ctgctgtgca gaaactgttt gaagtcgaag
     2521 tcgaagtcgt taacaccctg gtagttaaag ggaaagttaa acgtcacgga cagcgtatcg
     2581 gtcgtcgtag cgactggaaa aaagcttacg tcaccctgaa agaaggccag aatctggact
     2641 tcgttggcgg cgctgagtaa gtcggaggag taatacaatg gcagttgtta aatgtaaacc
     2701 gacatctccg ggtcgtcgcc acgtagttaa agtggttaac cctgagctgc acaagggcaa
     2761 accttttgct ccgttgctgg aaaaaaacag caaatccggt ggtcgtaaca acaatggccg
     2821 tatcaccact cgtcatatcg gtggtggcca caagcaggct taccgtattg ttgacttcaa
     2881 acgcaacaaa gacggtatcc cggcagttgt tgaacgtctt gagtacgatc cgaaccgttc
     2941 cgcgaacatc gcgctggttc tgtacaaaga cggtgaacgc cgttacatcc tggcccctaa
     3001 aggcctgaaa gctggcgacc agattcagtc tggcgttgat gctgcaatca aaccaggtaa
     3061 caccctgccg atgcgcaaca tcccggttgg ttctactgtt cataacgtag aaatgaaacc
     3121 aggtaaaggc ggtcagctgg cacgttccgc tggtacttac gttcagatcg ttgctcgtga
     3181 tggtgcttat gtcaccctgc gtctgcgttc tggtgaaatg cgtaaagtag aagcagactg
     3241 ccgtgcaact ctgggcgaag ttggcaatgc tgagcatatg ctgcgcgttc tgggtaaagc
     3301 aggtgctgca cgctggcgtg gtgttcgtcc gaccgttcgc ggtaccgcga tgaacccggt
     3361 agaccaccca catggtggtg gtgaaggtcg taactttggt aagcacccgg taactccgtg
     3421 gggcgttcag accaaaggta agaagacccg cagcaacaag cgtactgata aattcatcgt
     3481 acgtcgccgt agcaaataat tttagaggat aagccatgcc acgttctctc aagaaaggtc
     3541 cttttattga cctgcacttg ctgaagaagg tagagaaagc ggtggaaagc ggagacaaga
     3601 agcccctgcg cacttggtcc cgtcgttcaa cgatctttcc taacatgatc ggtttgacca
     3661 tcgctgtcca taatggtcgt cagcacgttc cggtatttgt aaccgacgaa atggtcggtc
     3721 acaaactggg tgaattcgca ccgactcgta cttatcgcgg ccacgctgct gataaaaaag
     3781 cgaagaagaa ataaggtagg aggaagagat ggaaactatc gctaaacatc gccatgctcg
     3841 ttcttctgct cagaaggttc gccttgttgc tgacctgatt cgcggtaaga aagtgtcgca
     3901 ggctctggat attttgacct acaccaacaa gaaagcggct gtactggtca agaaagttct
     3961 ggaatctgcc attgctaacg ctgaacacaa cgatggcgct gacattgacg atctgaaagt
     4021 tacgaaaatt ttcgtagacg aaggcccgag catgaagcgc attatgccgc gtgcaaaagg
     4081 tcgtgcagat cgcatcctga agcgcaccag ccacatcact gtggttgtgt ccgatcgctg
     4141 agactctgga gactagcaat gggtcagaaa gtacatccta atggtattcg cctgggtatt
     4201 gtaaaaccat ggaactctac ctggtttgcg aacaccaaag aattcgctga caacctggac
     4261 agcgatttta aagtacgtca gtacctgact aaggaactgg ctaaagcgtc cgtatctcgt
     4321 atcgttatcg agcgtccggc taagagcatc cgtgtaacca ttcacactgc tcgcccgggt
     4381 atcgttatcg gtaaaaaagg tgaagacgta gaaaaactgc gtaaggtcgt agcggacatc
     4441 gctggcgttc ctgcacagat caacatcgcc gaagttcgta agcctgaact ggacgcaaaa
     4501 ctggttgctg acagcatcac ttctcagctg gaacgtcgcg ttatgttccg tcgtgctatg
     4561 aagcgtgctg tacagaacgc aatgcgtctg ggcgctaaag gtattaaagt tgaagttagc
     4621 ggccgtctgg gcggcgcgga aatcgcacgt accgaatggt accgcgaagg tcgcgtaccg
     4681 ctgcacactc tgcgtgctga catcgactac aacacctctg aagcgcacac cacttacggt
     4741 gtaatcggcg ttaaagtgtg gatcttcaaa ggcgagatcc tgggtggtat ggctgctgtt
     4801 gaacaaccgg aaaaaccggc tgctcagcct aaaaagcagc agcgtaaagg ccgtaaataa
     4861 ggagcgtcgc tgatgttaca accaaagcgt acaaaattcc gtaaaatgca caaaggccgt
     4921 aaccgcggtc tggcgcaggg tacggatgtt agcttcggca gcttcggtct gaaagctgtt
     4981 ggccgtggtc gtctgactgc ccgtcagatc gaagcagcac gtcgtgctat gacccgtgca
     5041 gttaagcgtc aaggtaagat ctggatccgt gtgttcccgg acaaaccgat cactgaaaag
     5101 ccgctggcag tgcgtatggg taaaggtaaa ggtaacgtgg agtattgggt tgccttgatt
     5161 cagccgggta aagtcctgta tgaaatggac ggtgttccgg aagagctggc ccgtgaagca
     5221 ttcaagctgg cagcagcgaa actgccgatt aaaaccacct ttgtaactaa gacggtgatg
     5281 taatgaaagc aaaagagctg cgtgagaaga gcgttgaaga gctgaacacc gagctgctga
     5341 acctgctgcg tgagcagttc aacctgcgta tgcaggctgc aagtggccag ctgcaacagt
     5401 ctcacctgtt gaagcaagtg cgtcgcgatg tcgcacgcgt taagacttta ctgaacgaga
     5461 aggctggtgc gtaatgaccg ataaaatccg tactctgcaa ggtcgcgttg ttagcgacaa
     5521 aatggagaaa tccattgttg ttgctatcga acgttttgtg aaacacccga tctacggtaa
     5581 attcatcaag cgtacgacca aactgcacgt acatgacgag aacaacgaat gcggtatcgg
     5641 tgacgtggtt gaaatccgcg aatgccgtcc gctgtccaag actaaatcct ggacgctggt
     5701 tcgcgttgta gagaaagcgg ttctgtaata cagtacactc tctcgatacg aataaacggc
     5761 tcagaaatga gccgtttatt ttttctaccc atatccttga agcggtgtta taatgccgcg
     5821 ccctcgatat ggggattttt aacgacctga ttttcgggtc tcagtagtag ttgacattag
     5881 cggagcacta aaatgatcca agaacagact atgctgaacg tcgccgacaa ctccggtgca
     5941 cgtcgcgtaa tgtgtatcaa ggttctgggt ggctcgcacc gtcgctacgc aggcgtaggc
     6001 gacatcatca agatcaccat caaagaagca attccgcgtg gtaaggtcaa aaaaggtgat
     6061 gtgctgaagg cggtagtggt gcgcaccaag aagggtgttc gtcgcccgga cggttctgtc
     6121 attcgcttcg atggtaatgc ttgtgttctt ctgaacaaca acagcgagca gcctatcggt
     6181 acgcgtattt ttgggccggt aactcgtgag cttcgtagtg agaagttcat gaaaattatc
     6241 tctctggcac cagaagtact ctaaggagcg aatcatggca gcgaaaatcc gtcgtgatga
     6301 cgaagttatc gtgttaaccg gtaaagataa aggtaaacgc ggtaaagtta agaatgtcct
     6361 gtcttccggc aaggtcattg ttgaaggtat caacctggtt aagaaacatc agaagccggt
     6421 tccggccctg aaccaaccgg gtggcatcgt tgaaaaagaa gccgctattc aggtttccaa
     6481 cgtagcaatc ttcaatgcgg caaccggcaa ggctgaccgt gtaggcttta gattcgaaga
     6541 cggcaaaaaa gtccgtttct tcaagtctaa cagcgaaact atcaagtaat ttggagtagt
     6601 acgatggcga aactgcatga ttactacaaa gacgaagtag ttaaaaaact catgactgag
     6661 tttaactaca attctgtcat gcaagtccct cgggtcgaga agatcaccct gaacatgggt
     6721 gttggtgaag cgatcgctga caaaaaactg ctggataacg cagcagcaga cctggcagca
     6781 atctccggtc aaaaaccgct gatcaccaaa gcacgcaaat ctgttgcagg cttcaaaatc
     6841 cgtcagggct atccgatcgg ctgtaaagta actctgcgtg gcgaacgcat gtgggagttc
     6901 tttgagcgcc tgatcactat tgctgtacct cgtatccgtg acttccgtgg cctgtccgct
     6961 aagtctttcg acggtcgtgg taactacagc atgggtgtcc gtgagcagat catcttccca
     7021 gaaatcgact acgataaagt cgaccgcgtt cgtggtttgg atattaccat taccactact
     7081 gcgaaatctg acgaagaagg ccgcgctctg ctggctgcct ttgacttccc gttccgcaag
     7141 taaggtaggg ttactaaatg gctaagcaat caatgaaagc acgcgaagta aaacgcgtag
     7201 ctttagctga taaatacttc gcgaaacgcg ctgaactgaa agcgatcatc tctgatgtga
     7261 acgcttccga cgaagatcgt tggaacgctg ttctcaagct gcagactctg ccgcgtgatt
     7321 ccagcccgtc tcgtcagcgt aaccgctgcc gtcaaacagg tcgtccgcat ggtttcctgc
     7381 ggaagttcgg gttgagccgt attaaggtcc gtgaagccgc tatgcgcggt gaaatcccgg
     7441 gtctgaaaaa ggctagctgg taattgtcac caattgaatc acgggagtaa agacagatga
     7501 gcatgcaaga tccgatcgcg gatatgctga cccgtatccg taacggtcag gccgcgaaca
     7561 aagctgcggt caccatgcct tcctccaagc tgaaagtggc aatcgccaac gtgctgaagg
     7621 aagaaggttt tattgaagat tttaaagttg aaggcgacac caagcctgaa ctggaactta
     7681 ctctgaagta tttccagggc aaagctgttg tagaaagcat tcagcgtgtc agccgcccag
     7741 gtctgcgcat ctataaacgt aaagatgagc tgccgaaagt tatggcgggt ctgggtatcg
     7801 cagttgtttc tacctctaaa ggtgttatga ctgatcgtgc agcgcgccag gctggtcttg
     7861 gtggcgaaat tatctgctac gtagcctaat cggaggaaaa aatgtctcgt gttgctaaag
     7921 caccggtcgt tgttcctgcc ggcgttgacg taaaaatcaa cggtcaggtt attacgatca
     7981 aaggtaaaaa cggcgagctg actcgtactc tcaacgatgc tgttgaagtt aaacatgcag
     8041 ataataccct gaccttcggt ccgcgtgatg gttacgcaga cggttgggca caggctggta
     8101 ccgcgcgtgc cctgctgaac tcaatggtta tcggtgttac cgaaggcttc actaagaagc
     8161 tgcagctggt tggtgtaggt taccgtgcag cggttaaagg taatgtgatt aacctgtctc
     8221 tgggtttctc tcatcctgtt gaccatcagc tgcctgcggg tatcactgct gaatgtccga
     8281 ctcagactga aatcgtgctg aaaggcgctg ataagcaggt gatcggccag gttgcagcgg
     8341 atctgcgcgc ctaccgtcgt cctgagcctt ataaaggcaa gggtgttcgt tacgccgacg
     8401 aagtcgtgcg tatcaaagag gctaagaaga agtaaggtaa cactatggat aagaaatctg
     8461 ctcgtatccg tcgtgcgacc cgcgcacgcc gcaagctcca ggagctgggc gcaactcgcc
     8521 tggtggtaca tcgtaccccg cgtcacattt acgcacaggt aattgcaccg aacggttctg
     8581 aagttctggt agctgcttct actgtagaaa aagctatcgc tgaacaactg aagtacaccg
     8641 gtaacaaaga cgcggctgca gctgtgggta aagctgtcgc tgaacgcgct ctggaaaaag
     8701 gcatcaaaga tgtatccttt gaccgttccg ggttccaata tcatggtcgt gtccaggcac
     8761 tggcagatgc tgcccgtgaa gctggccttc agttctaagg tagaggtgta agatggctca
     8821 catcgaaaaa caagctggcg aactgcagga aaagctgatc gcggtaaacc gcgtatctaa
     8881 aaccgttaaa ggtggtcgta ttttctcctt cacagctctg actgtagttg gcgatggtaa
     8941 cggtcgcgtt ggttttggtt acggtaaagc gcgtgaagtt ccagcagcga tccagaaagc
     9001 gatggaaaaa gcccgtcgca atatgattaa cgtcgcgctg aataacggca ctctgcaaca
     9061 ccctgttaaa ggtgttcaca cgggttctcg cgtattcatg cagccggctt ccgaaggtac
     9121 cggtatcatc gccggtggtg caatgcgcgc cgttctggaa gtcgctgggg ttcataacgt
     9181 tctggctaaa gcatatggtt ccaccaaccc gatcaacgtg gttcgtgcaa ctattgatgg
     9241 cctggaaaat atgaattctc cagaaatggt cgctgccaag cgtggtaaat ccgttgaaga
     9301 aattctgggg aaataaacca tggcaaagac tattaaaatt actcaaaccc gcagtgcaat
     9361 cggtcgtctg ccgaaacaca aggcaacgct gcttggcctg ggtctgcgtc gtattggtca
     9421 caccgtagag cgcgaggata ctcctgctat tcgcggtatg atcaacgcgg tttccttcat
     9481 ggttaaagtt gaggagtaag agatgcgttt aaatactctg tctccggccg aaggctccaa
     9541 aaaggcgggt aaacgcttgg gtcgtggtat cggttctggc ctcggtaaaa ccggtggtcg
     9601 tggtcacaaa ggtcagaagt ctcgttctgg cggtggcgta cgtcgcggtt tcgagggtgg
     9661 tcagatgcct ctgtaccgtc gtctgccgaa attcggcttc acttctcgta aagcagcgat
     9721 tacagccgaa gttcgtctgt ctgacctggc taaagtagaa ggcggtgttg tagacctgaa
     9781 cacgctgaaa gcggctaaca ttatcggtat ccagatcgag ttcgcgaaag tgatcctggc
     9841 tggcgaagta acgactccgg taactgttcg tggcctgcgt gttactaaag gcgctcgtgc
     9901 tgctatcgaa gctgctggcg gtaaaatcga ggaataagta gcagatggct aaacaaccgg
     9961 gattagattt tcaaagtgcc aaaggtggct taggcgagct gaaacgcaga ctgctgtttg
    10021 ttatcggtgc gctgattgtg ttccgtattg gctcttttat tccgatccct ggtattgatg
    10081 ccgctgtact tgccaaactg cttgagcaac agcgaggcac catcattgag atgtttaaca
    10141 tgttctctgg tggtgctctc agccgtgctt ctatctttgc tctggggatc atgccgtata
    10201 tttcggcgtc gatcattatc cagctgctga cggtggttca cccaacgttg gcggaaatta
    10261 agaaagaagg ggagtctggt cgtcgtaaga tcagccagta cacccgctac ggtactctgg
    10321 tgctggcaat attccagtcg atcggtattg ctaccggtct gccgaatatg cctggtatgc
    10381 aaggcctggt gattaacccg ggctttgcat tctacttcac cgctgttgta agtctggtca
    10441 caggaacgat gttcctgatg tggttgggcg aacagatcac tgaacgaggt atcggcaacg
    10501 gtatttcaat cattatcttc gccggtattg tcgcgggact cccgccagcc attgcccata
    10561 ctatcgagca agcgcgtcaa ggcgacctgc acttcctcgt gttgctgttg gttgcagtat
    10621 tagtatttgc agtgacgttc tttgttgtat ttgttgagcg tggtcaacgc cgcattgtgg
    10681 taaactacgc gaaacgtcag caaggtcgtc gtgtctatgc tgcacagagc acacatttac
    10741 cgctgaaagt gaatatggcg ggggtaatcc cggcaatctt cgcttccagt attattctgt
    10801 tcccggcgac catcgcgtca tggttcgggg gcggtactgg ttggaactgg ctgacaacaa
    10861 tttcgctgta tttgcagcct gggcaaccgc tttatgtgtt actctatgcg tctgcaatca
    10921 tcttcttctg tttcttctac acggcgttgg ttttcaaccc gcgtgaaaca gcagataacc
    10981 tgaagaagtc cggtgcattt gtaccaggaa ttcgtccggg agagcaaacg gcgaagtata
    11041 tcgataaagt aatgacccgc ctgaccctgg ttggtgcgct gtatattacc tttatctgcc
    11101 tgatcccgga gttcatgcgt gatgcaatga aagtaccgtt ctacttcggt gggacctcac
    11161 tgcttatcgt tgttgtcgtg attatggact ttatggctca agtgcaaact ctgatgatgt
    11221 ccagtcagta tgagtctgca ttgaagaagg cgaacctgaa aggctacggc cgataattgg
    11281 tcgcccgaga agttacggag agtaaaaatg aaagttcgtg cttccgtcaa gaaattatgc
    11341 cgtaactgca aaatcgttaa gcgtgatggt gtcatccgtg tgatttgcag tgccgagccg
    11401 aagcataaac agcgccaagg ctgatttttt cgcatatttt tcttgcaaag ttgggttgag
    11461 ctggctagat tagccagcca atcttttgta tgtctgtgcg tttccatttg agtatcctga
    11521 aaacgggctt ttcagcatgg aacgtacata ttaaatagta ggagtgcata gtggcccgta
    11581 tagcaggcat taacattcct gatcataagc atgccgtaat cgcattaact tcgatttatg
    11641 gcgtcggcaa gacccgttct aaagccatcc tggctgcagc gggtatcgct gaagatgtta
    11701 agatcagtga gctgtctgaa ggacaaatcg atacgctgcg tgacgaagtt gccaaatttg
    11761 tcgttgaagg tgatctgcgc cgtgaaatca gcatgagcat caagcgcctg atggatcttg
    11821 gttgctatcg cggcttgcgt catcgtcgtg gtctcccggt tcgcggtcag cgtaccaaga
    11881 ccaacgcacg tacccgtaag ggtccgcgca aaccgatcaa gaaataatcg gggtgattga
    11941 ataatggcaa aggcaccaat tcgtgcacgt aaacgtgtaa gaaaacaagt ctctgacggc
    12001 gtggctcata tccatgcttc tttcaacaac accatcgtga ctatcactga tcgtcagggt
    12061 aacgcgttgg gttgggcaac agccggtggt tccggtttcc gtggttctcg caaatccact
    12121 ccgtttgcag ctcaggttgc agcagagcgt tgcgctgacg ccgtgaaaga atacggcatc
    12181 aagaatctgg aagttatggt taaaggtccg ggtccaggcc gcgaatctac tattcgtgct
    12241 ctgaacgccg caggtttccg catcactaac attactgatg tgactccgat ccctcataac
    12301 ggttgtcgtc cgccgaaaaa acgtcgcgta taacgcctcg ttttccaggt ttgttggaga
    12361 aagaaaatgg caagatattt gggtcctaag ctcaagctga gccgtcgtga gggcaccgac
    12421 ttattcctta agtctggcgt tcgcgcgatc gataccaagt gtaaaattga acaagctcct
    12481 ggccagcacg gtgcgcgtaa accgcgtctg tctgactatg gtgtgcagtt gcgtgaaaag
    12541 caaaaagttc gccgtatcta tggtgtgctg gagcgtcagt tccgtaacta ctacaaagaa
    12601 gcagcacgtc tgaaaggcaa caccggtgaa aacctgttgg ctctgctgga aggtcgtctg
    12661 gacaacgttg tataccgtat gggcttcggt gccactcgtg cagaagcacg tcagctggtt
    12721 agccataaag caattatggt aaacggtcgt gttgttaaca tcgcttctta tcaggttagt
    12781 ccgaatgacg ttgtaagcat tcgtgagaaa gcgaagaagc agtctcgcgt gaaagccgct
    12841 ctggagctgg ctgagcagcg tgaaaagcca acttggctgg aagttgatgc tggcaagatg
    12901 gaaggtacgt ttaagcgtaa gccggagcgt tctgatctgt ctgcggacat taacgaacac
    12961 ctgatcgtcg agctttactc caagtaaagc ttagtaccaa agagaggaca caatgcaggg
    13021 ttctgtgaca gagtttctaa aaccgcgcct ggttgatatc gagcaagtga gttcgacgca
    13081 cgccaaggtg acccttgagc ctttagagcg tggctttggc catactctgg gtaacgcact
    13141 gcgccgtatt ctgctctcat cgatgccggg ttgcgcggtg accgaggttg agattgatgg
    13201 tgtactacat gagtacagca ccaaagaagg cgttcaggaa gatatcctgg aaatcctgct
    13261 caacctgaaa gggctggcgg tgagagttca gggcaaagat gaagttattc ttaccttgaa
    13321 taaatctggc attggccctg tgactgcagc cgatatcacc cacgacggtg atgtcgaaat
    13381 cgtcaagccg cagcacgtga tctgccacct gaccgatgag aacgcgtcta ttagcatgcg
    13441 tatcaaagtt cagcgcggtc gtggttatgt gccggcttct acccgaattc attcggaaga
    13501 agatgagcgc ccaatcggcc gtctgctggt cgacgcatgc tacagccctg tggagcgtat
    13561 tgcctacaat gttgaagcag cgcgtgtaga acagcgtacc gacctggaca agctggtcat
    13621 cgaaatggaa accaacggca caatcgatcc tgaagaggcg attcgtcgtg cggcaaccat
    13681 tctggctgaa caactggaag ctttcgttga cttacgtgat gtacgtcagc ctgaagtgaa
    13741 agaagagaaa ccagagttcg atccgatcct gctgcgccct gttgacgatc tggaattgac
    13801 tgtccgctct gctaactgcc ttaaagcaga agctatccac tatatcggtg atctggtaca
    13861 acgtaccgag gttgagctcc ttaaaacgcc taaccttggt aaaaaatctc ttactgagat
    13921 taaagacgtg ctggcttccc gtggactgtc tctgggcatg cgcctggaaa actggccacc
    13981 ggcaagcatc gctgacgagt aaccggatca caggttaagg ttttactgag aaggataagg
    14041 tcatgcgcca tcgtaagagt ggtcgtcaac tgaaccgcaa cagcagccat cgccaggcta
    14101 tgttccgcaa tatggcaggt tcactggttc gtcatgaaat catcaagacg actctgccta
    14161 aagcgaaaga gctgcgccgc gtagttgagc cgctgattac tcttgccaag actgatagcg
    14221 ttgctaatcg tcgtctggca ttcgcccgta ctcgtgataa cgagatcgtg gcaaaactgt
    14281 ttaacgaact gggcccgcgt ttcgcgagcc gtgccggtgg ttacactcgt attctgaagt
    14341 gtggcttccg tgcaggcgac aacgcgccga tggcttacat cgagctggtt gatcgttcag
    14401 agaaagcaga agctgctgca gagtaatctg aagcaacgta aaaaaacccg ccccggcggg
    14461 tttttttata cccgtagcat ccccacttat ctacaatagc tgtactcttt ttgttcatcc
    14521 cctggagtat ttatgtggtt acttgaccag tgggcagagc gtcatatagc agaagcgcaa
    14581 gcgaaaggtg agtttgataa tctggcaggt agcggcgaac cattgatact ggatgatgat
    14641 tctcatgtgc caccggaatt acgtgcgggg tatcgcttgc tgaagaatgc tggttgctta
    14701 ccgccagaac ttgagcagcg gagggaagca attcagcttc tggatatcct gagaggtatc
    14761 cgtcacgatg atccgcaata tcaagaggtt agccgtcgat tgtcattact ggaattgaag
    14821 ctgcgacaag ctggatttag taccgatttt ttacgcggcg attatgctga caagttgttg
    14881 gacaaaatca acgataacta gtggagtatg tatgtatcgc attggtgagc tggcaaaaat
    14941 ggcggaagta acacctgaca cgattcgtta ttacgaaaaa cagcagatga tggagcatga
    15001 agtgcgtact gaaggcgggt ttcgcctgta taccgaaagc gatctccagc gattgaaatt
    15061 tatccgccat gccagacaac taggtttcag tctggagtcg atccgcgagt tgctgtcgat
    15121 ccgcatcgat cctgaacacc atacctgtca ggagtcaaaa ggcattgtgc aggaaagatt
    15181 gcaggaagtc gaagcacgga tagccgagtt gcagagtatg cagcgttcct tgcaacgcct
    15241 taacgatgcc tgttgtggga ccgcccatag cagtgtttat tgttcgattc ttgaagctct
    15301 tgaacaaggg gcgagtggcg ttaagagtgg ctgttgattt tttgcactgg caggattaca
    15361 ctcgcgccgt aaaataacca attggagttt ttatgagccg atatcagcat actaaagggc
    15421 agataaggga taatgcgata gaagcattac tacatgatcc cttattccga cagcgcgtag
    15481 agaaaaataa gaaggggaaa ggcagttaca tgcgaaaagg aaaacatggc aatcggggga
    15541 actgggaggc cagtggcaag aaagtaaatc acttttttac cactggtctt ctgctttcag
    15601 gcgcttgtta agagcggtta ttctgctctt tcagcaaatc acgaatttct gccagtaata
    15661 cttcttcttt agttggtgca ggtgcggcag ctggttcttc ttttttccga ttcagtttgt
    15721 tgattagctt aatcgccata aagatggcaa aggccacaat cagaaaatca aagacgtttt
    15781 gaatgaagac accgtaatgc atcacaacag cagggatatc cccctgcgca tcgcgtagcg
    15841 tgacagcaaa ctgtttaaag tcgatcccgc caattaataa gcccagaggt ggcatgatga
    15901 tatcggcaac cagtgaagat acaatcttcc cgaatgccgc accgataatg acacccaccg
    15961 ccaaatccac cacgttcccg cgcatcgcaa attcgcgaaa ttctttaata atgctcatgt
    16021 tattctccct atgaagccga caaccataag tctaacaaat gttaagccat tttcctgcca
    16081 ggaatgaatt taattaatcc ttgataatca aagggaaata ataaggcgtc attagacgcc
    16141 ttattaatta caagaagaaa gggcttggct ggaagagtct ttcgacgtcg gtaataaact
    16201 ttttatctgt gaggaacata attacgtgat cgccttgctc aatgcgcaga ttgtcattgg
    16261 caatcatcac gtcgtttcca cgtaccaccg ctccaataat cgttcctggc ggtagcttga
    16321 tttcgtcaat gactctgccg acaacgcgtg aggtgctttc atcaccgtga gcaacggctt
    16381 caatagcttc tgctacgccg cggcgcaatg aggaaacacc aacaatatct gcttttcgca
    16441 catggctaag caacgcagaa atagttgctt gttgtggtga aattgcaata tcgatcacgc
    16501 tcccctgaac cagatccaca taagcgcgac gctggatcaa taccatcacc tttttcgcac
    16561 ccatacgttt ggcaagcatg gcggacatga tattggcctc gtcatcgttg gtgacggcaa
    16621 taaacagatc aacttgatcg atatgttctt cggccagcag ttcttgatcc gacgcatcac
    16681 caaaaaagac gatcgtattc tgcaactttt ccgccagttc ggcagcgcgc tgctgattac
    16741 gttcgatgag tttaacgctg taatcttttt ccagacgacg cgccagccct gcaccgatat
    16801 taccgccacc aaccagcatg atccgcttat acggtttttc cagtcgctgt aattcactca
    16861 tcaccgcgcg gatatgctgt gaagcggcaa taaagaacac ttcatcacca gcttcaacaa
    16921 tggtcgaacc ttgtggacga atggggcgat cgtggcggaa aattgctgcc acacgagtat
    16981 cgatatgtgg catatgttcg cgcatggtcg aaagtgcatt accaatcagc gggccgccat
    17041 aataggcttt aactacagcg aggctgactt taccctcagc gaagttcacc acctgcaatg
    17101 cgcctggata ctcaatcagt cggtaaatat tatcgataac caactgctct ggtgcgatca
    17161 gatgatcaat cggtacagca tctgaatgaa atagcttatc ggcatcgcgt acgtagtctg
    17221 gtgagcggat gcgagcgatg cgattagggg tgttgaaaag tgagtaggct acctggcagg
    17281 caaccatatt ggtttcatct gaactggtta cagcaaccag catatcggcg tcgtcggcac
    17341 ctgcctcccg caatacgcgt ggatgagagc catgcccctg cacgactcgc aggtcaaatt
    17401 tatcctgcaa ggtccgcaga cgctcaccgt tggtatcgac aacagtaata tcgttgttct
    17461 cgccaaccag gttttccgcc agtgtgccgc caacctggcc ggcacccaga atgataattt
    17521 tcatcagtct cgacccgtta tctcatcact ttttgattag cttagcgtaa aagaagccgt
    17581 cgccctcttc ggcaccaggc agattttgtt tacccggttg ctctggtgtt cctgtttcgc
    17641 aaagttcggc atcagcggta cgttgcaaaa aagctttaat ctgcaggcta ttctcttccg
    17701 gtaacaccga acaggtggca tagaccaggg ttccaccgga ttttaaatgc ggccaaatgg
    17761 cgtcgagaat ttcagactgc aattgcgcga gttccgggat atcgcgatcg cgacgtaacc
    17821 atttaatatc cggatggcga cgaatcaccc ccgttgctga acaaggcgca tctaataaaa
    17881 tgcgatcaaa ctgttgctcg ccacaccatt gggaagggta acggccatca ccttgtttca
    17941 cggttgcctt cataccaagg cgttttaaat tgtcgtaaac gcgagagagg cgctgttcgt
    18001 caatatcaac cgcaacaacc tgcgcttctg gtgccacctc aaggatatgc gttgttttac
    18061 cgccgggggc ggcacaaaga tccagaatgt gttcaccgtt ttgtggcgca agccaggtca
    18121 tgcaaccttg tgctgatgca tcctgaacgg taacccatcc gtcttcaaaa ccaggtagcg
    18181 catgaacagg tgcaggtgtt tccagacgta cagcatcagg gtaatccgca tgcgggaaac
    18241 ctttcattcc tgcttcatcc agcaatgcaa gccagctgtc gcgggaatga tgcgtacgat
    18301 taacgcgcag ccacattggc ggacgctggt tattggcttc gacgatggat tgccactgct
    18361 ctggatacgc tttttgcaga cgcttcagca accaggaagg atgcagataa cgtgcatcac
    18421 tggcattaaa cttggctaat aactcttctt gctgacgctg gaactggcgt aatacaccgt
    18481 taatcaaccc tttaagttgc ggacgcttaa ttgcgatagc gccttcaacc gtttcagcca
    18541 gcgcagcatg aggtggaatg cgggtataaa gcagttgata caaaccaacc ataatcaggt
    18601 aatgcacagt gcgctgtttg ccggtcatcg gacgggccat taacttatta atcagccagt
    18661 ctaactgcga aagcgtacgc agtacgccaa agcacaactc ttgaagaagt gctttgtctt
    18721 tatcagaaac tttttgctgg agcggtggca gaatgttgct taatgattgc ccttgctcga
    18781 cgacttgttc aacggcctgg gccgccatgc tacgtaaatt acgttgtttt ttcataagta
    18841 taaaaatgcc cggtaagacc gggcttagaa gagtggacta tcaggccaga cggttgcccg
    18901 gaacaaacca ttcccgacga gaattcagga ggtcttgcgc gctcatcgct ttcttacccg
    18961 caggttgtaa cgagagcaga ttcaggattc catcaccagt tgcaacctga atgccttgtt
    19021 tgttggcttc aaggatcgtt cctggtgcag cgttggttgc cgtatcaatg accgacgctt
    19081 tccagacttt aaccggctgt ctttcaattt ccagccagct cattggccat ggattgaaag
    19141 cgcgaatgta gcgttcaagc tgtgctgccg aaagtgacca gtcaatacgc gcttcttctt
    19201 tactcaactt ttcggcgtaa gtgacaagag tttcgtcctg aacttctggt ttcgccgtgc
    19261 cgtctgccag ttgtttcaac gtggtgataa gcccttgtgg gccaagctct gccagcttgt
    19321 cgtacagcgt accactggta tcttctgcag taatcgggca ggagagctta tagagcatgt
    19381 caccggtgtc taaaccgaca tccatttgca taatggtcac accagtttct gcatcacccg
    19441 cccatagtga gcgttggatt ggtgcagcac cgcgccagcg tggcagcagt gaaccatgaa
    19501 cgttgataca gccaagacgc ggcatctcca gcactgcttt aggcagaatt aagccatagg
    19561 cgacgacgac cataacatca gcctgcagat cggcgaccag ttgctggttt tcttgtggac
    19621 gcagggaaac aggttgaaaa acgggcagac ctttttcctc agccagaact ttaaccgggc
    19681 tgggcatcag ttttttaccg cgtcctgccg gtcggtctgg ctgggtgaac acgccaacga
    19741 cgttatgacc agaagacaac agcgcgtcga gatgacgcgc tgcaaagtca ggtgtacccg
    19801 caaaaataat acgtagtgat tctgacacgt tagttcttat ccttaagccc gggctttcag
    19861 acgatccagt ttttcaactt tctgacgaat acgttgttgt ttcaacggtg acagataatc
    19921 cataaacagt ttgccgacca ggtgatccat ctcatgctga atacagatgg ctaacagacc
    19981 gtctgcttcc aattcaaatg gtttaccgtc gcggtccagg gcgcgaattt taactttctc
    20041 tgcgcgtggc actaaagcac gttgttcagg gatcgacagg caaccttctt caatgcctgt
    20101 ttcgccgctt ttttctaaaa gctccggatt gattaacacc agccgttcgt cacggttttc
    20161 cgaaacatca ataacaatga tacgttgatg gatatcaacc tgggttgccg ccaggccaat
    20221 accttcttct gcgtacatcg tctcgaacat atcatcgacg atacgctgaa tttctgcatt
    20281 cacttcttct accggtttag caactttgcg aagccgctcg tccggaatat gtaacacttg
    20341 caaaactgac ataaatctcc agagatgtgt tcaggagtta gaaagattat ttcttctatt
    20401 ctagacaaat ccccctctga ttgacagcat cactgaccaa tcgcaaagat tgctaaggct
    20461 gcttatggca gggagataag gatggtcgat acagatattt ggctgcgttt aatgagcatc
    20521 agcagcttgt acggcgatga tatggtccgt atagctcact ggctggcaaa acagtcgcat
    20581 attgatgcgg ttgtattgca gcaaacaggg cttacattgc ggcaggcaca acgctttctt
    20641 tcatttccgc gaaagagtat cgaaagctca ctttgttggt tggagcaacc caaccatcat
    20701 ttaatccctg cggacagcga attttatcct cctcaacttc tggcgacgac agattacccc
    20761 ggcgcactgt ttgttgaagg agaactgtac gcgctgcatt catttcagct tgccgtagtg
    20821 gggagtcggg cgcattcatg gtatggcgag cgatggggac gattattttg cgaaactctg
    20881 gccacgcgtg gagtgacaat tacgagtgga ctggcgcgtg gaatcgatgg tgtggcgcat
    20941 aaagcggcct tacaggtaaa tggcgtcagc attgctgtat tggggaacgg acttaatacc
    21001 attcatccgc gccgccatgc ccgactggct accagtttgc ttgaacatgg tggggcactt
    21061 gtctcggaat ttcccctcga tgtttcaccc cttgcttaca atttcccacg aagaaatcgc
    21121 attatcagtg gtctaagtaa aggtgtactg gtggtggaag cggctttgcg cagtggttcg
    21181 ttggtgacag cacgttgtgc gcttgagcag gggcgtgaag tttttgcctt gccaggacca
    21241 atagggaatc cgggaagcga agggcctcac tggttaataa aacaaggtgc gattcttgtg
    21301 acggaaccgg aagaaattct ggaaaacttg caatttggat tgcactggtt gccagacgcc
    21361 cctgaaaatt cattttattc accagatcag caagacgtgg cattgccatt tcctgagctc
    21421 ctggctaacg taggagatga ggtaacacct gttgacgtcg tcgctgaacg tgccggccaa
    21481 cctgtgccag aggtagttac tcaactactc gaactggagt tagcaggatg gatcgcagct
    21541 gtacccggcg gctatgtccg attgaggagg gcatgccatg ttcgacgtac taatgtattt
    21601 gtttgaaacc tatattcaca cagaagctga gttgcgtgtg gatcaagaca aacttgaaca
    21661 ggatcttacc gacgcaggat ttgatcgaga agatatctac aatgccctgc tttggctgga
    21721 aaagcttgcg gattatcagg aaggactgac agaaccgatg caactggcct ctgatccact
    21781 ctccatgcgt atttataccc ctgaggagtg tgaaagactg gatgccagct gtcgtggttt
    21841 tctgcttttc cttgagcaaa ttcaggtgct taaccttgaa atccgtgaaa tggtgataga
    21901 gcgagtgctc gcgctggata ccgcagagtt cgacctggaa gacctgaaat gggtaatcct
    21961 gatggtgttg ttcaatattc cgggctgcga aaatgcgtac cagcaaatgg aagaattact
    22021 ctttgaagtg aatgaaggta tgctgcatta atcatttctt taattcagca taagttgtta
    22081 tggcgaaatc agcactgttc acggtgcgta ataatgagtc ctgcccaaag tgcggggctg
    22141 aactggttat tcgatccggg aaacacggtc cgtttcttgg atgctcacag tatccggcgt
    22201 gtgactacgt ccgtcctctg aaatcttcag cggatggaca tattgtcaaa gttctggagg
    22261 ggcaggtttg ccctgcatgt ggcgcaaatc tggtattacg ccagggacgc tttggtatgt
    22321 ttattggttg cagtaactac cctgaatgcg aacataccga acttatcgat aaaccggacg
    22381 aaacagcaat tacatgcccc caatgtcgga cgggccatct ggtccagcgc cgctcccgtt
    22441 atggcaaaac atttcactcc tgtgatcgct acccggagtg tcaatttgcc attaacttca
    22501 aacccatagc tggagaatgc cctgagtgtc attatccact actcatcgaa aagaaaaccg
    22561 cgcagggtgt aaaacacttt tgtgccagta aacaatgtgg aaagccggtt tcggcggaat
    22621 aataacgtga ataataacct gcaaggagac gctatcgcag ctgcgataga tgtcctcaat
    22681 gaagaacgtg tcatcgccta tccaacggaa gccgttttcg gtgttgggtg cgatcctgat
    22741 agcgaaacag cagtgatgag actgttggag ttaaaacagc gtccggttga taaggggctg
    22801 attttaatcg cggcaaatta cgagcagctt aaaccctata ttgatgacac catgctgact
    22861 gatgcgcagc gtgaaaccat tttttcccgc tggccaggtc ctgtcacctt tgtctttccc
    22921 gcgcctgcga caacaccgcg ctggttgacg ggccgctttg attcgcttgc tgtacgagtc
    22981 accgaccatc cgttggtggt tgctttgtgc caggcttatg gtaaaccgct ggtttctacc
    23041 agtgccaact tgagtggatt gccaccttgt cgaacagtag acgaagttcg cgcacaattt
    23101 ggcgcggcgt tcccggttgt gcttggtgaa acgggggggc gtttaaatcc ttcagaaatc
    23161 cgcgatgccc tgacgggtga actgtttcga caggggtaac ataatggaaa cctatgctgt
    23221 ttttggtaat ccgatagccc acagcaaatc gccattcatt catcagcaat ttgctcagca
    23281 actgaatatt gaacatccct atgggcgcgt gttggcaccc atcaatgatt tcatcaacac
    23341 actgaacgct ttctttagtg ctggtggtaa aggtgcgaat gtgacggtac cttttaaaga
    23401 agaggctttt gccagagcgg atgagcttac tgaacgggca gcgttggctg gtgctgttaa
    23461 taccctcaag cggttagaag atggacgctt gcagggtgac aataccgatg gtataggttt
    23521 gttaagcgat ctggaacgtc tgtcttttat ccgtccgggt ttacgtattc tgcttatcgg
    23581 cgctggtgga gcatctcgcg gcgtactact accactcctt tccctggact gtgcggtgac
    23641 aatcactaat cggacggtat cccgtgcgga agagttggct aaattgtttg cgcacactgg
    23701 cagtattcag gcgttgggta tggacgaact ggaaggtcat gagtttgatc tcattattaa
    23761 tgcaacatcc agtggcatca gtggtgatat tccggcgatc ccgtcatcgc tcattcatct
    23821 aggcatttat tgctatgaca tgttctatca gaaaggaaaa actccttttc tggcatggtg
    23881 tgagcagcga ggctcaaagc gtaatgctga tggtttagga atgctggtgg cacaggcggc
    23941 tcatgccttt cttctctggc acggtgttct gcctgacgta gaaccagtta taaagcaatt
    24001 gcaggaggaa ttgtccgcgt gaatcaggcc atccagtttc cggacaggga agagtgggac
    24061 gagaataaaa aatgtgtatg ttttcccgct ctcgtgaatg gtatgcaact gacatgcgcg
    24121 atctctggcg agagtctggc gtatcgcttt actggagata cgccagaaca gtggttagcg
    24181 agttttcgtc agcatcgctg ggacctggaa gaagaagcgg aaaacttaat tcaggaacaa
    24241 agtgaagatg atcaaggctg ggtctggtta ccctgatcca gatattcgtc cttccatttc
    24301 acgtaattat tcgcggaata gcgtaaccca gccttctctt catcacttaa cgggcggatc
    24361 tgtttgacgg ggctaccgag atacagatat ccgctctcca gccgtttatt ttgtgggacc
    24421 agactacccg caccaatcat cacatcatct tctactattg cgccatcaag taaaattgag
    24481 cccatcccaa ccaaaactcg attgccaatg gtgcagccgt ggagcatcac cttgtgacca
    24541 acagtgacat cttcgccaat ggttaatggg ttgccatctg ggttgtacga ggatttatga
    24601 gtgacatgca acatactgcc atcctggata ttggtgcgtg ctccgatctg tacataatgt
    24661 acatctccac gaatcacaac gagcggccag atccccacat catcagccag acgaacgcca
    24721 ccaatcacga cactgctatc gtcgatcatt acgcgctgac cgatttgtgg aaaaagatcg
    24781 cggtatgggc gtaaaacatc agacatactt acctcagcaa taaatgattt actaatgact
    24841 ttgggggcat tattggcctt gtgcaagtct tttagtatgc aaaaaagcac cgttttgtgt
    24901 gcgattgcag caaaaagggt gaaaaaacaa caaacagaaa aaaagatcaa aaaaatactt
    24961 gtgcaaaata ttgggatccc tataatgcgc ctccgttaag acgacaacgt gaaacacttc
    25021 acaggatggt cggaacaacg aagagaaaaa atcctgaaat tcagggttga ctctgaaaga
    25081 ggaaagcgta atatacgcca cctcgcgaca gtgagctgaa agccgcgtcg caactgctct
    25141 ttaacaattt atcagacaat ctgtgtgggc actcgaagat acggattctt aacgtcgcaa
    25201 gacgaaaaat gaataccaag tctcaagagt ga
//
LOCUS       DAJVUJ010000045        25118 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.045, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000045 DAJVUJ010000000
VERSION     DAJVUJ010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25118
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.045"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..393
                     /gene="hyfA"
                     /locus_tag="OQQ48_001713"
     CDS             <1..393
                     /gene="hyfA"
                     /locus_tag="OQQ48_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HCS1952523.1"
                     /translation="QLNESLCIGCKLCAVVCPFGAISASGSRPVNAPAQYVFQADGSL
                     KDGEENAPTQHALLRWEPGVQTVAVKCDLCNFLPEGPACVRACPNQALRLITDDSLQR
                     QMKEKQRLAASWFANGGEDPLSLTQEQH"
     gene            393..2410
                     /gene="hyfB"
                     /locus_tag="OQQ48_001714"
                     /pseudo
     CDS             393..2410
                     /gene="hyfB"
                     /locus_tag="OQQ48_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            2421..3368
                     /gene="hyfC"
                     /locus_tag="OQQ48_001715"
     CDS             2421..3368
                     /gene="hyfC"
                     /locus_tag="OQQ48_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HCS1952524.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLLLTPLFTGISRQIRARMHSRRG
                     PGIWQDYREIHKLFKRQEVAPISSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            3385..3495
                     /locus_tag="OQQ48_001716"
     CDS             3385..3495
                     /locus_tag="OQQ48_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoin racemase"
                     /protein_id="HCS1952525.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGFCSPH"
     gene            3507..4614
                     /locus_tag="OQQ48_001717"
                     /pseudo
     CDS             3507..4614
                     /locus_tag="OQQ48_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001442177.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit D"
     gene            4626..5276
                     /gene="hyfE"
                     /locus_tag="OQQ48_001718"
     CDS             4626..5276
                     /gene="hyfE"
                     /locus_tag="OQQ48_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HCS1952526.1"
                     /translation="MIGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            5281..6861
                     /gene="hyfF"
                     /locus_tag="OQQ48_001719"
     CDS             5281..6861
                     /gene="hyfF"
                     /locus_tag="OQQ48_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HCS1952527.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVDVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPK
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            6851..8566
                     /gene="hyfG"
                     /locus_tag="OQQ48_001720"
     CDS             6851..8566
                     /gene="hyfG"
                     /locus_tag="OQQ48_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HCS1952528.1"
                     /translation="MNVNSSSNSGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLHKDAMDYRL
                     RPEPTTDSETYAFINEGNSDARVIPVGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANW
                     PVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRN
                     RSPLK"
     gene            8576..9115
                     /gene="hyfH"
                     /locus_tag="OQQ48_001721"
     CDS             8576..9115
                     /gene="hyfH"
                     /locus_tag="OQQ48_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HCS1952529.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTNKA
                     DLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLRAQASVCPECKQ
                     RATLINDDTDVPLVAKEQL"
     gene            9112..9870
                     /gene="hyfI"
                     /locus_tag="OQQ48_001722"
     CDS             9112..9870
                     /gene="hyfI"
                     /locus_tag="OQQ48_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HCS1952530.1"
                     /translation="MSPVLTQHVSQPITLDEQTQKMKRHLLQDIRRSAYVYRVDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            9863..10276
                     /gene="hyfJ"
                     /locus_tag="OQQ48_001723"
     CDS             9863..10276
                     /gene="hyfJ"
                     /locus_tag="OQQ48_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HCS1952531.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHRSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTENEDWLAQVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFALLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            10306..12318
                     /gene="hyfR"
                     /locus_tag="OQQ48_001724"
                     /pseudo
     CDS             10306..12318
                     /gene="hyfR"
                     /locus_tag="OQQ48_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416986.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
     gene            12340..13184
                     /locus_tag="OQQ48_001725"
                     /pseudo
     CDS             12340..13184
                     /locus_tag="OQQ48_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
     gene            complement(13222..14283)
                     /locus_tag="OQQ48_001726"
     CDS             complement(13222..14283)
                     /locus_tag="OQQ48_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCS1952532.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            14496..15959
                     /gene="bepA"
                     /locus_tag="OQQ48_001727"
     CDS             14496..15959
                     /gene="bepA"
                     /locus_tag="OQQ48_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HCS1952533.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADNADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHLTQRHLARAMEDQQRSTPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            15980..16339
                     /gene="arsC"
                     /locus_tag="OQQ48_001728"
     CDS             15980..16339
                     /gene="arsC"
                     /locus_tag="OQQ48_001728"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HCS1952534.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(16477..17223)
                     /gene="hda"
                     /locus_tag="OQQ48_001729"
     CDS             complement(16477..17223)
                     /gene="hda"
                     /locus_tag="OQQ48_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HCS1952535.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(17273..18562)
                     /gene="uraA"
                     /locus_tag="OQQ48_001730"
     CDS             complement(17273..18562)
                     /gene="uraA"
                     /locus_tag="OQQ48_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCS1952536.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEEADITDK"
     gene            complement(18648..19274)
                     /gene="upp"
                     /locus_tag="OQQ48_001731"
     CDS             complement(18648..19274)
                     /gene="upp"
                     /locus_tag="OQQ48_001731"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCS1952537.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            19598..20635
                     /gene="purM"
                     /locus_tag="OQQ48_001732"
     CDS             19598..20635
                     /gene="purM"
                     /locus_tag="OQQ48_001732"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCS1952538.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            20635..21273
                     /gene="purN"
                     /locus_tag="OQQ48_001733"
     CDS             20635..21273
                     /gene="purN"
                     /locus_tag="OQQ48_001733"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCS1952539.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDVTARVQTQEHAIYPLVISWFADGRLKIHENAAWLDGQRLPPQGYAADE"
     gene            21445..23511
                     /gene="ppk1"
                     /locus_tag="OQQ48_001734"
     CDS             21445..23511
                     /gene="ppk1"
                     /locus_tag="OQQ48_001734"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HCS1952540.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNPEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPHLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            23516..25057
                     /gene="ppx"
                     /locus_tag="OQQ48_001735"
     CDS             23516..25057
                     /gene="ppx"
                     /locus_tag="OQQ48_001735"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HCS1952541.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRVEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
BASE COUNT         5718 a         6576 c         6684 g         6140 t
ORIGIN      
        1 caactcaacg aaagcctctg tattggctgc aagctttgcg ccgtggtctg cccatttggc
       61 gcaatcagcg cttcaggaag ccgtccggtg aatgcccctg cgcaatatgt ttttcaggct
      121 gacggctcac tcaaagatgg cgaagaaaac gcgccgaccc aacatgcttt gctgcgctgg
      181 gaaccgggtg tccagaccgt cgcggtgaaa tgcgacctgt gtaatttctt gccagaaggt
      241 ccggcctgcg ttcgcgcttg cccgaatcag gcgttacggc tgattaccga cgatagcctg
      301 caacgtcaga tgaaagaaaa acagcgcctt gccgcaagct ggtttgccaa tggcggggag
      361 gatccccttt ccctcactca ggagcaacac taatggatgc cctgcaatta ttaacctggt
      421 cgctgatcct ctttctgttt gccagtctgg cttcgctgtt tttactcggt ctggacagac
      481 tggctattaa gctttccggc atcacatcgc tggtgggcgg cgtgattggc atcatcagcg
      541 gaattacgca attacatgct ggtgtaactt tagtcgcccg ttttgccacc ccttttgact
      601 ttgccgattt aaccctgcga atggatagcc tctcggtatt tatggtgctg gttatctcct
      661 tgctggtggt ggtttgttcg ctctattcat tgacttatat gcgcgaatac gagggcaaag
      721 gcgcggcggc gatgggcttc tttatgaatc ttttcatcgc atcgatggtt gccctgctgg
      781 tgatggacaa cgctttttgg ttcatcgtgc tgtttgaaat gatgtcgctg tcttcctggt
      841 ttctggtcat tgccaggcag gataaaacgt cgatcaacgc tggcatgctc tactttttta
      901 tcgcccacgc cggatcggtg ctgattatga tcgccttctt gctgatgggg cgcgaaagcg
      961 gcagcctcga ttttgccagt ttcgcacgct ttcactttct ccggggctgg cgtcggcgat
     1021 gtttctgctg gcctttttcg gttttggcgc gaaagccggg atgatgccgt tgcacagctg
     1081 gttgccgcgc gctcaccctg ccgcaccatc gcacgcttcg gcgttgatgt ctggcgtaat
     1141 ggtcaaaatc ggtattttcg gcatcctgaa agtagcgatg gatctgctgg cgcaaacggg
     1201 tttgcctctg tggtggggca ttctggtgat ggcgatcggc gcaatctccg cgctcctggg
     1261 cgtgctatat gcgctggcgg aacaggatat caaacggctg ctggcctgga gtaccgtcga
     1321 aaacgtcggc attattttgc tggcagtcgg tgtggcgatg gacggtctgt cactgcacga
     1381 cccgctgctc accgtggttg gactgctcgg cgcactgttt catctgctca accatgcgct
     1441 gttcaaaggg ctgctatttc tcggcgcggg cgcgattatt tcgcgtttgc atacccacga
     1501 catggaaaaa atgggggcac tggcgaaacg gatgccgtgg acagccgcag catgcctgat
     1561 tggttgcctc gcgatatcag ccattcctcc gctgaatggt tttatcagcg aatggtacac
     1621 ctggcagtcg ctgttctcac taagtcgtgt ggaagccgta gcgctacaac ttgcgggtcc
     1681 tattgctatg gtaatgctgg cagtcactgg tgggctggca gtaatgtgct tcgtcaaaat
     1741 gtacggtatt actttctgtg gtgcgccgcg cagtacacac gctgaagagg cacaggaagt
     1801 gccaaatacg atgatcgtcg ccatgctact gctcgcggca ctctgcgtat taattgcgct
     1861 tagtgccagt tggctggcac cgaagataat gcatattgcc catgcgttta ccaatacccc
     1921 tcccgtcact gtcgccagcg gaatagcact tgtacccggc acgtttcata cacgggtcac
     1981 tccttcatta ctgttgctgt tactactggc gatgcctttg ctgcctggcc tttactggct
     2041 gtggtgtcgt tcgcgccgcg cagcgtttcg tcgcacagga gatgcctggg catgcggcta
     2101 cggctgggaa aacgcgatgg ctccgtcagg caatggcgtg atgcagccgc tgcgtgtggt
     2161 cttctctgcg ttatttcgtc tacgacaaca gctcgaccct acgctgaggc taaataaaga
     2221 tcttgcgcac gtcaccgcca gggctcagag cacagaaccc ttctgggatg agcgggtgat
     2281 ccgccccatc gtgagcgcca cccaacggct ggccaaagaa atacagcatc tgcaaagcgg
     2341 cgactttcgt ctctattgcc tgtatctagt tgccgcactg gttgtgctgc taatcgctat
     2401 tgccgtctaa ggaaatcacc atgagacaaa ctctttgcga cggatatctg gtcatttttg
     2461 cgttagcaca ggccgtgatt ctgctgctgc taaccccgct ttttacgggt atttcccggc
     2521 agatacgcgc gcgtatgcac tcccgccgcg ggccggggat ctggcaggat tatcgcgaga
     2581 tccacaaact gtttaaacgc caggaagttg cgcctatatc ttcaggtctg atgttccgcc
     2641 tgatgccgtg ggtattaatc agcagcatgc tggtgctggc gatggcccta ccacttttta
     2701 ttaccgtttc cccttttgcg ggcggcggcg atctgatcac ccttatctat cttcttgccc
     2761 tgtttcgttt tttctttgct ctttccggac tggataccgg aagcccgttt gcgggagtcg
     2821 gtgccagtcg cgagttgacg ctcggcattc tggtcgaacc catgctcatt ctctcactgc
     2881 tggtattggc gctgatagca ggttccacgc atatcgagat gatcagcaat acgctggcaa
     2941 cgggctggaa ctcgccgcta accaccgtac tggcgttact ggcctgtggt tttgcctgct
     3001 tcattgagat gggaaaaatt ccctttgatg ttgctgaagc agaacaggaa ttacaggaag
     3061 gcccgctgac cgaatattcc ggtgccgggc tggcgctagc gaaatggggg ctggggctga
     3121 aacaggtcgt gatggcagca ctgtttgtgg ccctgtttct gccctttggg cgcgcgcaag
     3181 aactttctct cgcctgcctg ctgacttcac ttgtcgttac gctgctcaag gttttgctga
     3241 tttttgtact ggcctcaatc gcagaaaaca cgctggcacg cgggcgtttt ttactcattc
     3301 accatgtgac ctggcttggc ttcagccttg ctgcgcttgc atgggtcttc tggttaaccg
     3361 gtctgtaagg agcactgacg gaatatggaa aatcttgctc tgacgacgtt aatgctgcct
     3421 tttatcggcg cactggtcgt ttcgttttcg ccacaacgtc gggccgccga atgggggttt
     3481 tgttcgccgc actgaccacg ctgtgcatgt tgtcgctgat ctccgcgttt tatcaggccg
     3541 ataaagttgc cgtcacgttg acgttggtca acgtggggga tgtggcattg tttggcctgg
     3601 tcattgatcg cgtgagtacg ctgattctgt ttgtggtggt gtttctcggt ttgctggtca
     3661 cgatctactc cacgggttat ctgacggata aaaatcgcga acacccgcat aacggcacga
     3721 atcgttatta cgcatttttg ctggtgttta tcggcgcgat ggcgggactg gtactctcct
     3781 cgacgctgct cggtcagttg ttgttttttg aaattagggc ggctgctcct gggcgttgat
     3841 cagttattac cagagcgata aagcgcagcg ttcaacacta aaagcgttgc ttatcaccca
     3901 tatcggttcg ttggggttgt atcttgccgc cgccacgctg tttttgcaga ccggaacgtt
     3961 tgtgcttagc gcgatgagcg agttacatgg cgacgcacgt tatctggttt atggcggcat
     4021 cctgtttgcc gcgtggggga aatcggccca gctaccgatg caagcgtggc taccggacgc
     4081 aatggaagcg ccaacaccga tcagagccta tctccacgcc gcatcgatgg tgaaagtggg
     4141 cggctggcac tggagggcag catcgcctac atcgtcaacc acgcgttcgc taaaagcctg
     4201 tttttccttg tagcaggtgc gctgagttac agctgcggca cgcgcttgtt gccgcgtctg
     4261 cgtggcgtat tgcactccct gccgttgcca ggcgtgggtt tctgcgtggc agcgctggcg
     4321 attaccggtg tgccgccgtt caacggcttc ttcagtaaat tcccgctgtt tgccgccggt
     4381 tttgcgttgt cagtggagta ctggatcctg ctgcccgcca tgattctgct gatgattgaa
     4441 tcggtcgcca gtttcgcctg gtttattcgc tggtttggtc gcgttgtgcc tggcaaaccg
     4501 agcgaggccg tcgccgatgc cgcaccgctg ccagggtcaa tgcgcctggt gttgattgta
     4561 ctgattgtga tgtcgctgat ttccagcgta atcgccgcga cctggttgca gtaaggagat
     4621 gatgaatgat cggttctatg atcgtaaata atctggcggg actgatgatg ctgacatcgc
     4681 tgtttgtgat tagcgtcaaa agctatcgcc tgtcatgcgg attttacgcc tgccagtcgc
     4741 tggtgctggt gtctattttc gccactctct cgtgcctgtt cgccgcagag caactgctga
     4801 tctggtccgc cagcgccttt atcaccaaag tgctgctggt accgttaatc atgacttacg
     4861 ctgcacgaaa tattccccag aacatcccgg aaaaagcgtt attcggtccg gcaatgatgg
     4921 cactgctcgc ggcgttaatt gtcctgcttt gcgcatttgt cgttcagccc gtgaagctac
     4981 cgatggctac cgggctgaaa ccggcgctgg cggtagcgtt aggtcatttt ctgcttggcc
     5041 tgctgtgcat tgtcagccag cgcaatatcc tgcggcaaat ttttggttac tgcctgatgg
     5101 aaaacggctc ccatctggtg ctggcgcttc ttgcctggcg agcaccggaa ctggtggaaa
     5161 taggtatcgc taccgacgcc atcttcgccg tcattgtgat ggtgttactg gcaagaaaaa
     5221 tatggcgtac ccacggcacg ctggacgtga acaacttgac cgcgctgaag ggataatgag
     5281 atgagttatt ctgtgatgtt cgctttactc ctgctcacgc cgctgctttt ttcgctgctc
     5341 tgttttgcct gccggaaacg ggggctctct gcgacttgta cggtgactgt attacatagc
     5401 ttagggatca cactgctgct gattctggca ctctgggtgg tccaaactgc cgctgatgca
     5461 ggagaaatat tcgctgcggg actgtggctt catattgatg gtctgggcgg tttgttcctc
     5521 gccattcttg gtgtgattgg ctttctcacc ggtgtttact cgattggcta catgcgtcat
     5581 gaagtggcac acggcgagct ttcacccgtt acactgtgcg attactacgg tttcttccat
     5641 ctgtttttgt tcaccatgct gctggttgtt accagcaata acctgattgt gatgtgggcg
     5701 gcgatcgaag ccaccacctt aagctcggcg tttctggtag gcatttacgg tcagcgttca
     5761 tcgctggaat cagcatggaa gtacatcatt atttgtactg ttgatgtcgc ttttggtctg
     5821 ttcggtaccg tgctggtata cgccaacgcc gccagcgtta tgccgcaggc agaaatggcg
     5881 atattctgga gcgaggttct taagcaatcg tccttgcttg acccaacatt aatgctgttg
     5941 gcctttgtgt ttgtgctaat tggctttggt accaaaaccg ggctatttcc catgcacgcc
     6001 tggctgccgg atgctcacag tgaagcgccg agtccggtca gcgccctgct ctccgccgta
     6061 ttgctgaact gcgcgctgtt ggtgctgatt cgctattaca tcattatttg ccaagccatc
     6121 ggcagcgatt tccccaaccg gttgttgctc atcttcggca tgttgtcggt tgccgtggcg
     6181 gcatttttca ttctggtaca gcgggacatt aagcgtctgc tggcgtactc cagcgtggag
     6241 aacatggggc tggtcgcggt ggcgctaggc attggcgggc cgctgggaat ttttgccgcg
     6301 ctgctgcaca ccttaaacca cagtctggca aaaacgctgc tgttctgcgg ttccggcaat
     6361 gtactgctca agtacggcac gcgcgatctc aacgtcgtct gtgggatgct caaaatcatg
     6421 ccatttaccg ccgtgctgtt tggcggcggt gcgctggcgc tggcagggat gccgcccttc
     6481 aacatttttc ttagcgaatt tatgaccgtt accgcaggac tggcacgtaa tcacctgctg
     6541 attatcgtcc tgctgttatt gctgttaacg ctggtgctgg cgggcctggt acggatggct
     6601 gcgcgggtgt taatggcgaa accgccgaag gccgttaacc ggggtgatct cggctggttg
     6661 accacctcgc caatggtgat tctgctggtc atgatgctgg cgatgggaac gcatattcca
     6721 caacctgtca tcaggatcct ggcgggcgct tccactatag tcctctcagg gacgcacgac
     6781 ctgcctgcac aacgtagcac ctggcatgat tttttgcctt caggcaccgc atctgtttcg
     6841 gagaaacaca gtgaacgtta attcatcgtc aaatagtggc gaagcgattc tcgccgccct
     6901 gaaaacgcag ttccccggcg cggtactgga tgaagagcga caaacgcctg aacaggtcac
     6961 cattacggtg aaaatcaatc tgctgcctga cgttgtacat tatctttatt atcaacatga
     7021 tggctggctt ccagtcctgt ttggcaacga cgagcggaca cttaacggtc attacgcggt
     7081 ttattatgcc ctttcaatgg aaggggccga aaaatgctgg atcgtggtga aggcactggt
     7141 cgatgccgac agtcgggagt ttccgtcagt cacaccgcgc gtccctgccg cggtctgggg
     7201 cgagcgagaa attcgcgata tgtacgggct gattccggtt ggcctgccgg atcagcgtcg
     7261 cctggtgttg cccgatgact ggccggaaga tatgcatccg ctgcacaaag atgcgatgga
     7321 ttatcgactg cgccctgaac cgacgacaga ttccgaaacg tatgcgttta tcaatgaggg
     7381 caacagcgat gcgcgggtga tccctgtcgg cccgctgcat atcacctccg atgaaccggg
     7441 tcacttccgc ttgtttgtgg atggcgagca aattgtcgat gctgattacc gcctgtttta
     7501 tgtccatcga ggcatggaga aactggcaga aacgcggatg ggctacaacg aagtgacctt
     7561 cttatcggac cgcgtgtgtg ggatttgcgg ttttgcccac agtgtggcct ataccaactc
     7621 ggttgaaaat gcactgggga ttgaggtgcc gcaacgagcg cataccattc gctcgattct
     7681 gctggaagtc gaacggctac acagtcattt gctcaacctt ggcctctcct gccattttgt
     7741 tggttttgat accggcttta tgcaattttt ccgcgtgcgg gaaaagtcga tgacgatggc
     7801 ggaattgctg accgggtcgc gtaaaaccta cggtctgaat ctgattggtg gtgttcgccg
     7861 cgatattctc aaagagcaac gtctgcaaac gctgaaactg gtgcgcgaga tgcgcgccga
     7921 cgtgtcggag ctggtagaga tgctgcttgc tacgccgaat atggaacaac gcactcaggg
     7981 cattggcatt ctcgaccgac aaatcgcccg tgattatagc cctgtagggc cgctgatccg
     8041 cggcagtggt tttgcccgtg atttgcgctt tgatcacccc tacgccgact acggcaatat
     8101 tccaaaaaca ctgtttacct tcaccggcgg cgatgttttc tcccgcgtga tggtccgtgt
     8161 caaagagacg tttgattcgc tggcaatgct ggaatttgcc ctcgacaaca tgccggatac
     8221 cccactgctg accgaaggct ttagctataa acctcacgca ttcgcgctgg gctttgttga
     8281 agcgccacgc ggtgaagacg tgcactggag catgctcggt gataaccaaa aattgttccg
     8341 ctggcgctgc cgtgccgcca cctacgccaa ctggccggtg ttgcgttaca tgctgcgcgg
     8401 caataccgtt tctgacgcac cgctgattat cggtagcctt gatccctgct actcctgtac
     8461 cgaccgtgtg acgctggtag atgtgcgcaa gcgccagtca aaaaccgtgc cgtataaaga
     8521 gatcgaacgc tacggcattg atcgtaaccg ttcgccgctg aagtaaggac agaagatgct
     8581 gaagttactg aaaactatta tgcgcgccgg aaccgcgacg gtgaaatatc ccttcgcgcc
     8641 actggaggtc agcccaggct ttcgcgggaa accggacctg atgcccagcc aatgtattgc
     8701 ctgcggcgcc tgtgcttgtc cggcaaatgc gctgactatc cagaccgacg accagcaaaa
     8761 ttcgcgcacc tggcagctct atctggggcg ttgtatttac tgcggacgtt gtgaagaagt
     8821 gtgcccgacc agagccatcc agcttaccaa taactttgaa ctgaccgtca ccaataaagc
     8881 cgatctctat acccgcgcga cgttccatct acaacgttgc agccgttgcg aacgcccgtt
     8941 tgccccgcaa aaaaccgtcg cactggctgc tgaattgttg gcacagcaac aaaatgcgcc
     9001 acaaaaccgc gaaatgttgc gggcgcaagc gagcgtctgc ccggagtgca aacaacgcgc
     9061 gacgctgatc aacgacgata cagatgtacc gctggtggct aaggagcagc tatgagtcca
     9121 gtgcttacac aacatgtcag ccagcccatc acgctggatg agcaaacgca aaagatgaag
     9181 cggcatttgc tacaggatat ccgtcgctca gcctacgttt atcgcgtcga ttgcggcggc
     9241 tgcaacgcct gtgaaatcga aatttttgct gccatcaccc ccgtattcga cgcagaacgt
     9301 tttggcatta aggttgtttc atcaccgcgt cacgccgata ttttgttatt tactggcgca
     9361 gtcacccggg cgatgcgtat gcctgcactt cgggcgtatg agtctgcccc cgatcataaa
     9421 atctgtgttt cctacggcgc gtgcggtgtc ggcggcggta ttttccacga tctctacagc
     9481 gtctggggcg gcagcgacac cattgtcccc attgatgttt ggatccccgg ctgcccgcca
     9541 acaccggccg ccaccattca cggtttcgcc gtggcgctcg gtttgctgca acagaagatt
     9601 cacgctgtgg attatcgcga tcccaccggg gtgactatgc aaccgttgtg gccacagatc
     9661 ccgccatcac agcgtatcgc cattgagcga gaagcgcggc ggctggcggg ctatcgtcag
     9721 gggcgagaaa tttgcgatcg gctcctgcgc catttaagcg acgatcctac aggaaatcgg
     9781 gttaacacct ggttgcgcga tgccgacgat ccacgtctca atagtatcgt tcagcaactc
     9841 tttcgcgtac tccgggggtt acatgactga agagtgcggg gaaattgttt tctggacgct
     9901 gcgaaaaaag tttgtcgcca gtagcgacga gatgccggaa caccgctctc aggtaatgta
     9961 ttactcgctg gctatcggcc atcacgttgg cgtgattgat tgtctgaatg tcgccttccg
    10021 ctgcccactg acggaaaacg aagattggct tgcacaggtc gaagaggagc aagcccgacg
    10081 taagatgctg ggggtgatga cctttggtga gattgttatt gacgccagcc acaccgccct
    10141 gttgacccgg gcattcgcgc tactggcaga tgacgcgacg tctgtgtggc aggcgcgtag
    10201 cattcaattc attcatctgt tggatgaaat tgtgcaggaa ccggccatct atctgatggc
    10261 cagaaaaatt gcgtgagaag gatttctcat taataaggac tgttgatggc tatgtcagac
    10321 gaggcgatgt ttgccccgcc acaaggaata acaattgaag cggtaaacgg aatgctcgcg
    10381 gagcggttag cacagaaaca cggtaaggcg tctttattac gcgccttcat cccgatgccg
    10441 ccgccgttca gcccggtaca acttattgaa ctgcatgtta tcaaaagcaa cttctattac
    10501 cgctaccatg atgatggcag cgatgtgacg gcaacaacag agtatcaggg cgagatggtc
    10561 gattattcgc gtcacgccgt ccttctcggc agtagtggaa tggcggagct acgctttatt
    10621 cgtacccacg gcagccgttt tactccctag gattgcacac tgtttaactg gctggcacgc
    10681 attatcactc cggttctgca atcatggctc aatgatgaag aacaacaggt ggcgctgcgt
    10741 ttgctggaga aagatcgcga tcatcatcgg gtactggttg atattactaa tgcagtgctg
    10801 tcacatcttg atctcgacga tctgatcgct gacgtcgctc gtgagatcca tcattttttc
    10861 ggtctggctt cagtcagtat ggtactgggc gatcatcgaa agaacgagaa gtttagcctg
    10921 tggtgcagcg atctttctgc ctcacattgt gcgtgtctgc cacgcaatat gcctggcgac
    10981 agtgtattgc tgacacaaac gctacaaacc cgacaaccga ccttgacgca ccgtgcagac
    11041 gatctgtttc tctggcaacg cgacccgtta ttactcttac ttgcatctaa cggctgcgaa
    11101 tctgcgctcc ttataccgct tacctttggc aaccatacac cgggtgcatt gttgctggcg
    11161 catacctctt ccactctctt tagtgaggaa aactgccagc tactacaaca catagccgat
    11221 cgcatcgcta ttgccgttgg caatgccgat gcctggcgta gcatgaccga tttgcaggaa
    11281 agtttgcagc aagaaaacta ccagcttagc gagcagctcc tttcgaatct gggcatcggt
    11341 gacattatct atcaaagcca ggcaatggaa gacctgctcc agcaggtaga tattgtagcg
    11401 aagagcgaca gtacggtgtt gatttgtggt gaaaccggaa ccggcaaaga ggtgatcgcc
    11461 agagcgctcc atcaacttag cacgcgacgc gacaaaccgc tggtcaaaat caactgcgct
    11521 gccatccccg ccagtcttct ggaaagtgag ttattcggtc atgacaaagg ggcgtttact
    11581 ggtgcgatta atacacatcg tggtcgtttt gaaattgccg atggcggcac gttgtttctc
    11641 gatgaaattg gcgatctgcc gttagaactt cagcctaaac tgctgcgcgt attgcaggaa
    11701 cgggagattg agcgtctcgg cgggagtaga acgatcccgg tgaatgtcag agtcattgcc
    11761 gccaccaacc gtgatttgtg gcaaatggtt gaagatcgcc agtttcgcag caatctcttt
    11821 tatcgcctga atgtcttccc attggaattg ccgccgctac gcgaccgtcc ggaagatatc
    11881 cctcttttag caaaacattt cacgcaaaaa atggcgcgcc atatgaatcg cgcaattgac
    11941 gccatcccga ccgaggcgct acgccagttg atgtcgtggg actggccggg caacgtgcac
    12001 gagctggaaa acgtgattga gcgagcggta ctgctgactc gcggtaacag tctgaattta
    12061 catcttaatg tccgacaaag ccgtttactg ccgacgctaa atgaagattc agcgcttcgc
    12121 agttcaatgg cgcagttgct gcacccgacg acgccagaga atgacgaaga agaacgtcag
    12181 cgcattgttc aggtattgcg agaaaccaat ggcattgttg ccgggccccg tggcgcggcg
    12241 acacgattag ggatgaagcg caccacgctg ctgtcacgaa tgcagcgtct ggggatctcg
    12301 gttcgcgagg tgttgtaatc tgcttttgca ggagtatgca tgagaaacaa actctctttc
    12361 gacttgcagt tgagcgccag aaaagcggca atcgctgaac ggattgccgt ccataaaatt
    12421 gcccgcagta aagtgtcggt ctttttaatg gcgatgtccg ctggcgtgtt tatggcgatc
    12481 ggatttactt tttacctttc cgttatcgcc gatgccccgt cttcacaggc attaacccat
    12541 ctggtgggcg gcctttgctt tacactcggc tttattttgc tggcggtttg cggcacagcc
    12601 tgttcacctc gtcggtaatg acggtgatag caaaaagtcg gggcgttatt agttggcgaa
    12661 cttggctgat taacgcactt ctggtggcct gtggtaatct ggcaggtatt gcctgtttca
    12721 gtttgttaat ctggttttcc gggctggtga tgagtgaaaa cgcgatgtgg ggagtcgcgg
    12781 ttttacactg cgccgagggc aaaatgcatc atacatttac tgaatctgtc agcctcggca
    12841 ttatgtgcaa tctgatggtt tgcctggcgc tgtggatgag ttattgcggg cgttcgttat
    12901 gcgacaaaat cgtcgccatg attttgccca tcaccctgtt tgtcgccagt ggctttgagc
    12961 actgtatcgc caatttgttt gtgattccgt tcgccattgc cattcgccat ttcgcccccc
    13021 ccttctggca actggcgcac agtagcgcag acaattttcc ggcactgacg gtcagccatt
    13081 ttattaccgc caatctgctc ccggtgatgc tgggtaatat tatcggcggt gcagtgctgg
    13141 tgagtatgtg ttatcgggct atttatttac gtcaggaatc ctgaaaaatc agcccggcga
    13201 aacagttcgt cgggctgaag attattcttg cgcgatttgc ccatcgggcc aggcgtggat
    13261 cacggctttg atcagcgtcg ccaatggaat ggcgaagaat acgccccaga atccccacaa
    13321 accaccgaag atcaccaccg ataaaataat caccagcgga tgcaggttaa ccgcttcgga
    13381 gaacaacacc ggtactagca ggttgccgtc cagcgcctga ataatcagat acaccgcgaa
    13441 gcagctccag aattccgtgc ctgcgccaaa ctggaatagc gccacgccaa ccaccggaat
    13501 ggtcaccaca aatgcgccaa tgtacggaat aagaaccgag aagccgacca gcaccgccag
    13561 cagcagcgaa tagttcagcc caaagagcaa gaaccccagc caggtggcga tccccaccac
    13621 gatcatctcc agcactttgc cgcggatata gttggtgatt tgttgattca tctccttcca
    13681 cacctgtcct gccagtccac ggttgcgcgg cagcacccga cgaacggcgt tcagcatctg
    13741 ctctttgtct ttcagcagga agaagaccat caatggcacc agcaccagat agacggcaat
    13801 ggtcagcaaa ccgaccagcg aggcgaggga aattttcacc accgaatcgc ccatggtcag
    13861 catccgactg cgcatatttt cggccattgc atcaataatg cccgcgtcca ttaacgccgg
    13921 atagcggcgc ggcaacgtgg cggcaaagtc agaaagctta ttgagcatcc ccggcatatc
    13981 gcggattaag tagatgccct gttgccaggc gataggcagt accacgaacg ccatcagtag
    14041 caatataccg acaaaaacca ccaatacaat cgacgtcgcc cagcggcggg agcagccaat
    14101 agattgcagg cgcacggttg gccattccag caaataggcc agcacaatag ccaccagcaa
    14161 cggagcaagc aggccactaa agaaaaagat aatgccaaat ccggcaacta aaataaccag
    14221 caaggcaatc gcttccgggt cgctaaaacg gcggcgatac cattgcatca acatttcgag
    14281 cataaaaacc ttccctgaac ctcaagagcg ggattgcgat ccgcaattgt atcgaaatgt
    14341 cacaaaaaag acttcgcttt ttatgacgga ttcaggaaac tgaaaagtca tttgagtggg
    14401 ctaatcttcg ccgttacact caaaggcggc gcggtgggaa cgatatttca cagtatcggt
    14461 caaatgacta tcttcagaaa tacaggatag aggttatgtt caggcagttg aaaaaaaacc
    14521 tggttgcaac cctcattgct gctatgacca ttggtcaggt agccccggcg tttgccgaca
    14581 acgcagacac cttgccggat atgggaacct ccgcaggaag cacgctttcc attggtcagg
    14641 aaatgcagat gggcgactat tatgtccgcc agctacgcgg cagcgcgccg ttaattaatg
    14701 acccgctgtt aacgcaatat attaattcgc tggggatgcg tctggtttcg catgccaatt
    14761 cggttaagac accgtttcat ttttttctaa tcaacaacga cgaaattaac gcctttgctt
    14821 tctttggcgg caacgtggtg ctgcactctg ccctgttccg ttattccgat aacgaaagtc
    14881 aactggcttc agttatggcg cacgaaatct cccacctcac ccaacgtcac ctggcgcgag
    14941 cgatggaaga tcagcagcgc agcacgccgc tgacctgggt cggcgcgtta ggttctattt
    15001 tactggcgat ggccagtccg caggcgggga tggcggcgct gaccggtaca ctggcgggaa
    15061 cgcgtcaggg gatgatcagt ttcacccagc aaaatgaaca ggaagcggac cgcattggta
    15121 ttcaggtgct gcaacgctcg ggattcgatc cgcaggcgat gccaaccttc ctcgaaaaat
    15181 tactcgatca ggcgcgttac tcctcgcgcc cgccggaaat tttattgact cacccgttgc
    15241 cggaaagtcg tctggcagat gcccgcaacc gtgctaatca gatgcgaccg atggtggtgc
    15301 agtcgtcgga agatttctat ctggcgaaag cgcgcacact ggggatgtat aattccggac
    15361 gtaaccagct caccagtgat ttgctggatg aatgggcgaa aggaaacgtt cgtcagcaac
    15421 gagcggcgca atatggtcgt gctttacagg cgatggaagc caataaatac gacgaggcgc
    15481 gtaaaacgct gcaaccgtta ctggcggcag aacctggcaa cgcatggtat ctcgatctgg
    15541 ctaccgatat cgatcttggg caaaacaaag ccaatgaggc aatcaatcgc ctgaaaaatg
    15601 cccgtgattt gcgcaccaat ccggtgttgc agctcaacct ggcaaacgct tatctgcaag
    15661 gcggtcaacc acaagaagcg gccaatattc tcaatcgcta cacctttaat aataaagatg
    15721 acagcaacgg ctgggattta ctggcacagg cggaagccgc gctaaataac cgcgatcagg
    15781 agctggctgc gcgagcagaa ggttatgcgc tcgccggacg actcgatcag gccatttcgc
    15841 tgttgagtag cgccagttcg caggtgaaat taggcagcct gcaacaagcg cgttacgatg
    15901 cgcgcatcga ccagttgcgc cagctgcagg aacgctttaa gccttatacc aagatgtaat
    15961 tcgcattaaa ggaagaacaa tgaccaaaca ggtaaaaatc taccataacc cacgctgttc
    16021 aaagagccgg gaaacgctga acctgctgaa agaaaacggc gtggagccag aagtggtact
    16081 ctaccttgag acacccgccg atgcggcaac gctgcgcgat ttgctgaaaa tgctggggat
    16141 gaatagcgcc cgtgaattga tgcgccagaa agaggatctt tataaagaac tgaacctggc
    16201 tgacagttcg cttagcgaag aggcgctgat tcaggcaatg gtggataatc cgaagctgat
    16261 ggagcgcccg attgtggtcg cgaatggcaa agcgcggatt ggtcgtccac cggagcaggt
    16321 actggaaatc gtgggttaag atgtgctcca tcgctagttg aagcacattg ccggatgcga
    16381 cgcttgccat gtcttatccg gcctacgaat actatggatt aacttcgcgt agttcggata
    16441 aggcgttcgc gccgcatccg acaataaaca ccttatctac aacttcagaa tttctttcac
    16501 aaacggaatg gtcagcttac gttgcgcggt aatcgacgca cgatccaact gatccaacgt
    16561 cataaatagc gtgcgcattt ctctgtcgag ccgcttcagc aagaaacgcc ccacatcttc
    16621 cggcagttca aaaccacgca aacgcgcgcg taactgtagc gcctgcaact tatcttcatc
    16681 agaaagtggc tgcaatttgt agatctgccc ccagtcgagt cgcgacgcga gatccggtaa
    16741 tcccagattc aactgccgcg gtggacgatc gccggtgatc aacaaccgtg ttttgcccga
    16801 ttccagaatt cgattgtaga gatcgaaaat cgccatctcc cacaactcat cgcctgcaat
    16861 acactcaatg ttgtcgatac aaaccagcga caaatgctcc ataccgtcga gcacttccgg
    16921 aacaaaccaa gtgcgtttat ccagcgggac atagcccacc gcatcgccac gctgcgacaa
    16981 ttccgcgcaa gccgcgtgca gcagatggct gcgccccgcg ccttcgcgtg cccagagata
    17041 gatgtaaccg ctatgttcct gacgcagcac gttttgcagc gcggccagta aagaggagtt
    17101 atcccccggc cagaaacttg caaaggtttc gtcgtcagga agataaagtg gcaaagagag
    17161 ctgtgccggt gtgttcagag atacctcaac caggatttca caaaatcgcg agaagtttac
    17221 cacagaatcc catgatgttt gaaccgggca gcaacactgc ccggtcggta cattatttgt
    17281 ctgttatatc cgcctcttct gcgtccagca ccacttcttc agggcgcagc acactgatca
    17341 gtttgaaaat caaacttaag ccgataccga cgatggtcgc cagcgccata ccttttaact
    17401 ccgccgcgcc gatgtttacc ttcgcgccac tgacgccgat gatcaaaatc acggaagtca
    17461 ggatcaggtt ctgtgctttg ttgtaatcca ctttcgattc gatcaaaaca cgaataccgg
    17521 aagcaccgat gacaccataa agcagcagcg aaacgccgcc catcaccggc aatgggatca
    17581 tctggatagc ggcagccagt ttaccgacgc aggaaagcag gatagcgaaa atcgccgccc
    17641 cgccgataac ccaggtactg taaacacggg tgatcgccat cacgccaatg ttttctccgt
    17701 aagtggtatt tggcgtggag ccaaagaagc cggagatcac ggtcgacagg ccgttagcaa
    17761 acatcgaacg gtgcagacct ggatcgcgca gcagatcttt tttgacgata ttagccgtta
    17821 ctaccaggtg ccctacgtgt tcggcaataa ccactaacgc cgctggcaga atagtcagaa
    17881 tggcaaacca ctcgaagcgc ggcgtataga gggttggcag cgcaaaccag tgagcattaa
    17941 taatcggcgt ggtatcgaca attcccattg cgaaagagag cgcgtagccc accagcacgc
    18001 caattaaaat cgggataatt gccaggaaac cacgaaacag cacggaacct aaaaccgtga
    18061 ccgccagggt ggtaatagag atgatgatgg ttttggagtc tggcgtttgc ccttcagccg
    18121 ggagtaaacc cgccataccg gcagctacgc ccgccagctc cagaccgatg acggcaacga
    18181 ttgcgcccat tgccgcaggt ggaaacagca cgtccagcca gccggtcccc gctttcttca
    18241 cgataaaaga aaccaggcag aacagcacgc cgcacataat aaagccgccc agcgcgactt
    18301 cataccctaa cggcaacagt aacaataccg gtgaaataaa ggcaaagctg gaaccaagat
    18361 aagccggaat tttcccttta cagatgaaga gatacagcag cgttccaata ccgttaaata
    18421 acagtacagt cgccgggtta atatgaaata agacgggcac caggacggtt gcaccaaaca
    18481 tggcgaacaa atgttgcaaa ctaagcggga ttgtctgtaa aagtggcggt ctttcactca
    18541 ccccgatagc acggcgcgtc atagtattat cctctgtatt atgtgttata ggcgctttac
    18601 tcaaaaaaaa gccgactctt aaagtcggct ttaattattt ttattcttta tttcgtacca
    18661 aagattttgt caccggcatc gccgaggccc ggaataatgt atccgtgctc gttcagtccc
    18721 tgatcaatcg atgcggtata cagttcgacg tccgggtgcg ctttttccag cgcagcgata
    18781 ccttctggcg cagcgaccag caccagaact ttgatgctgc tgcagcccgc ttttttcagc
    18841 aggtcgatgg tcgcgataac ggaaccaccg gttgccagca ttgggtcaac gatcagcgcc
    18901 atacgctcat cgatgttaga aaccagtttc tggaagtacg gtaccggctc cagcgtttct
    18961 tcattacggt acataccgac aacgctgatg cgcgcgctcg gaacattttc cagcacaccg
    19021 tccatcatac caagacccgc acgcagaatt ggcacaacgg taattttctt acctttgatc
    19081 tggtcgattt ctaccgggcc gttccagcct tcgatagtta ctttttccgt ttcgaggtcg
    19141 gcggtcgctt cgtaagtcag caggctaccc acttcggaag cgagttcgcg aaagcgcttg
    19201 gtgctgatat cttgctcacg catcagtccc agcttgtgtt tgacgagtgg gtgtttgact
    19261 tccacgatct tcatactctt tctcctttga ggggcagcca caaaaaaatc gacggattat
    19321 acctcctttc ttcaaggcgg caatattctt ttcgttgact ttagtcaaaa tgataacgat
    19381 ttgagataaa gttattttat attcaaatgg ttatgaaaga agatcattcc atccgaaaac
    19441 taacctttgc cctggcacaa gtcttctttc gccgcgcgtc tggggaaaag acgtgcaaaa
    19501 aggttgtgca aagcagcctc gcaaacgttt gctttccctg ttagaattgc gccgaatttt
    19561 atttttctac cgcaagtaac gcgtggggac ccaagcagtg accgataaaa cctctcttag
    19621 ctacaaagat gccggtgttg atattgacgc gggtaatgct ctggttggaa gaatcaaagg
    19681 cgtagtgaag aaaacgcgtc gtccggaagt gatgggcggt ctgggcggct tcggtgcgct
    19741 gtgtgcattg ccgcaaaaat atcgtgaacc cgtgctggtt tccggcactg acggcgtagg
    19801 taccaagctg cgtctggcaa tggacttaaa acgtcacgac accattggta ttgatctggt
    19861 cgccatgtgc gttaatgacc tggtggtgca aggtgcagaa ccgctgtttt tcctcgacta
    19921 ttacgcaacc ggaaaactgg atgttgatac cgcttcagcg gtgatcagcg gcatcgcgga
    19981 aggttgtctg caatcgggct gttcactggt gggtggcgaa acggcagaaa tgccggggat
    20041 gtatcacggt gaagattacg atgtcgcggg tttctgcgtg ggcgtggtag aaaaatcaga
    20101 aatcatcgac ggctctaaag tcagcgacgg cgatgttctg attgcactcg gttccagcgg
    20161 tccgcactcg aacggctatt cgctggtgcg caaaattctt gaagtcagcg gttgtgatcc
    20221 gcaaaccacc gaacttgatg gtaagccatt agccgatcat ctgctggcac cgacccgcat
    20281 ttacgtgaag tcagtgctgg agttgattga aaaggtcgat gtgcatgcca ttgcgcacct
    20341 gaccggcggc ggcttctggg aaaacattcc acgcgtattg ccagataata ctcaggcagt
    20401 gattgatgaa tcttcctggc agtggccgga agtgttcaac tggctgcaaa cggcaggtaa
    20461 cgttgagcgc catgaaatgt atcgcacctt caactgcggc gtcgggatga ttattgccct
    20521 gcctgctccg gaagtggaca aagccctcgc cctgctcaat gccaacggtg aaaacgcgtg
    20581 gaaaatcggt atcatcaaag cctctgattc cgaacaacgc gtggttatcg aataatgaat
    20641 attgtggtgc ttatttccgg taacggaagt aatttacagg caattattga cgcctgtaaa
    20701 accaacaaaa ttaaaggcac cgtacgggca gttttcagca ataaggccga cgcgttcggc
    20761 cttgaacgcg cccgccaggc gggtattgca acgcatacgc tcatcgccag cgcgtttgac
    20821 agtcgtgaag cctatgaccg ggagttgatt catgaaatcg acatgtacgc acccgatgtg
    20881 gtcgtgctgg ctggttttat gcgcattctc agcccggcgt ttgtctccca ctatgccggg
    20941 cgtttgctga acattcaccc ttctctgctg ccgaaatatc ccggattaca cacccatcgt
    21001 caggcgctgg aaaatggcga tgaagagcac ggtacatcgg tgcatttcgt caccgatgaa
    21061 ctggacggtg gcccggttat tttacaggcg aaagtcccgg tatttgctgg tgatacggaa
    21121 gatgacgtca ccgcccgcgt gcaaacccag gaacacgcta tttatccact ggtgattagc
    21181 tggtttgccg atggtcgtct gaaaatacac gaaaacgccg cgtggctgga tggtcaacgt
    21241 ctgccgccgc agggctacgc tgccgacgag taatgccccc gtagttaaag cgccagctct
    21301 gccgctggcg tttttcaatt cacctgtaaa tcgcaagctc cagcagtttt tttccccctt
    21361 ttctggcata gttggacatc tgccaatatt gctcgccata atatccaggc agtgtcccgt
    21421 gaataaaacg gagtaaaagt ggtaatgggt caggaaaagc tatacatcga aaaagagctc
    21481 agttggttat cgttcaatga acgcgtgctt caggaagcgg cggacaaatc taacccgctg
    21541 attgaaagga tgcgtttcct ggggatctat tccaataacc ttgatgagtt ctataaagtc
    21601 cgcttcgctg aactgaagcg acgcatcatt attagcgaag aacaaggctc caactctcat
    21661 tcccgccatt tactgggcaa aattcagtcc cgggtgctga aagccgatca ggaattcgac
    21721 ggcctctaca acgagctatt gctggagatg gcgcgcaacc agatcttcct gattaatgaa
    21781 cgccagctct ccgtcaatca acaaaactgg cttcgtcatt attttaagca gtatctgcgt
    21841 cagcacatta cgccgatttt aatcaatcct gacactgact tagtgcagtt cctgaaggat
    21901 gattacacct atctggcggt ggaaattatc cgtggagaca ccatccgtta cgcgctgctg
    21961 gagatcccat cagataaagt gccgcgcttt gtgaatttac cgccagaagc gccgcgtcga
    22021 cgcaagccga tgattcttct ggataacatt ctgcgttact gccttgatga tattttcaaa
    22081 ggcttctttg attatgacgc gctgaatgcc tattcaatga agatgacccg cgatgccgaa
    22141 tacgatttag tgcatgagat ggaagccagc ctgatggagt tgatgtcttc cagtctcaag
    22201 cagcgtttaa ctgctgagcc ggtgcgtttt gtttatcagc gcgatatgcc caatgcgctg
    22261 gttgaagtgt tacgcgaaaa actgactatt tcccgctacg actccatcgt ccccggcggt
    22321 cgttatcata attttaaaga ctttattaat ttccccaatg tcggcaaagc caatctggtg
    22381 aacaaaccac tgccgcgttt acgccacatt tggtttgata aagcccagtt ccgcaatggt
    22441 tttgatgcca ttcgcgaacg cgatgtgttg ctctattatc cttatcacac ctttgagcat
    22501 gtgctggaac tgctgcgtca ggcttcgttc gacccgagcg tactggcgat taaaattaac
    22561 atttaccgcg tggcgaaaga ttcacgcatc atcgactcga tgatccacgc cgcacataac
    22621 ggtaagaaag tcactgtggt ggttgagtta caggcgcgtt tcgacgaaga agccaacatt
    22681 cactgggcga agcgcctgac cgaagcaggc gtgcacgtta tcttctctgc gccggggctg
    22741 aaaattcacg ccaaactgtt cctgatttca cgtaaagaaa acggtgaagt ggtccgttac
    22801 gcacacatcg ggaccgggaa ctttaacgaa aaaaccgcgc gtctttatac tgactattcg
    22861 ttgctgaccg cagatgcgcg tatcaccaac gaagtacggc gggtatttaa ctttattgaa
    22921 aacccatacc gcccagtgac atttgattat ttaatggtgt caccgcaaaa ctctcgccgt
    22981 ctgttatatg aaatggtaga ccgcgaaatc gccaacgcgc agcaagggct gcccagtggt
    23041 atcaccctga agctaaataa ccttgtcgat aaaggcctgg ttgatcgtct gtatgcggcc
    23101 tccagctccg gcgtaccggt taatctgctg gttcgcggaa tgtgttcgct gatccccaat
    23161 ccggaaggca ttagcgacaa cattcgtgcc atcagtattg ttgaccgtta ccttgaacat
    23221 gaccgggttt atatttttga aaatggcggc gataaaaagg tctacctttc ttccgccgac
    23281 tggatgacgc gcaatattga ttatcgtatt gaagtggcga caccgctgct cgatccgcac
    23341 ctgaagcagc gggtgctgga catcatcgac atattgttca gcgatacggt caaagcacgt
    23401 tatatcgata aagaactcag taatcgctac gttccccgcg gcaatcgccg caaagtacgg
    23461 gcgcagttgg cgatttacga ctacatcaaa tcactcgaac aacctgaata accctatgcc
    23521 aatacacgat aaatcccctc gtccgcagga gtttgctgcg gtcgatcttg gttcaaacag
    23581 ttttcacatg gtcatagccc gtgtggtaga tggtgccatg cagattattg gccgcctgaa
    23641 acagcgagtg catctggcgg acggcctggg gccagataat atgttgagtg aagaggcaat
    23701 gacgcgcggt ttgaactgtc tgtcgctgtt tgccgaacgg ctacaagggt tttctcctgc
    23761 cagcgtctgt atagtcggca cccatacgct gcgtcaggcg ctgaacgcca ctgactttct
    23821 gaaacgcgtg gaaaaggtca ttccctaccc gattgaaatt atttccggta atgaagaagc
    23881 ccgtctgatt tttatgggcg tggaacatac ccaaccggag aaaggtcgca aactggttat
    23941 tgatattggc ggcggatcta cggaactggt gattggtgaa aatttcgaac cgattctcgt
    24001 tgaaagccgc cggatgggtt gtgtcagctt tgcccagctt tacttccctg gcggggtcat
    24061 caataaagag aattttcagc gcgctcgtat ggcggcagca caaaaactgg aaactttaac
    24121 ctggcaattc cgtattcagg gctggaacgt ggcaatgggc gcttccggta ccataaaagc
    24181 cgcccatgaa gtgttaatgg aaatgggcga gaaagacggg ataattaccc cggaacgtct
    24241 ggaaaaactg gtaaaagaag ttttacgtca ccgtaatttc gcatcgctga gtttaccggg
    24301 tctttccgaa gagcggaaaa cagtcttcgt tcctggactg gcgattttat gcggtgtgtt
    24361 tgatgcttta gccatccgtg aactgcgcct ttctgacggg gcgttacgcg aaggcgtact
    24421 gtacgaaatg gaaggacgtt tccgtcatca ggatgtgcgt agtcgcaccg ccagcagcct
    24481 cgccaaccag tatcacatcg acagcgaaca ggcccgacgg gtgctggata ccactatgca
    24541 aatgtacgaa cagtggcggg aacagcaacc gaagctggcg catccgcaac tggaggcgct
    24601 actgcgatgg gccgccatgc tgcatgaggt cgggttgaat atcaaccaca gcggtttgca
    24661 tcgccactcc gcttatattc tgcaaaacag tgacttgccg ggttttaatc aggaacagca
    24721 gctgatgatg gcgacactgg tgcgctatca ccgcaaagcg attaaactcg acgatctgcc
    24781 gcgctttacc ttgtttaaga agaaacagtt cctgccactg attcagctat tgcgccttgg
    24841 cgtattactc aacaatcaac gtcaggcaac caccacaccg ccaacattga cactgattac
    24901 tgatgacagt cactggacac tgcgtttccc gcatgactgg tttagtcaga atgcgttggt
    24961 actgcttgat ctggaaaagg agcaagaata ctgggaaggc gtggctggct ggcggttgaa
    25021 aattgaagaa gaaagtacac ctgaaatcgc cgcttaatgc tttgcgggcc gacgagaaat
    25081 gtcggcccgc attattcagg cactttcgcg aatgggtt
//
LOCUS       DAJVUJ010000046        24904 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.046, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000046 DAJVUJ010000000
VERSION     DAJVUJ010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24904
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.046"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(38..376)
                     /locus_tag="OQQ48_001736"
                     /pseudo
     CDS             complement(38..376)
                     /locus_tag="OQQ48_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(492..1043)
                     /gene="maa"
                     /locus_tag="OQQ48_001737"
     CDS             complement(492..1043)
                     /gene="maa"
                     /locus_tag="OQQ48_001737"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HCS1952542.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKGVPDNVVVGGNPARIIKKL"
     gene            complement(1215..1433)
                     /gene="hha"
                     /locus_tag="OQQ48_001738"
     CDS             complement(1215..1433)
                     /gene="hha"
                     /locus_tag="OQQ48_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCS1952543.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(1459..1833)
                     /gene="tomB"
                     /locus_tag="OQQ48_001739"
     CDS             complement(1459..1833)
                     /gene="tomB"
                     /locus_tag="OQQ48_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HCS1952544.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNYGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(2379..5528)
                     /locus_tag="OQQ48_001740"
     CDS             complement(2379..5528)
                     /locus_tag="OQQ48_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HCS1952545.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(5551..6744)
                     /gene="acrA"
                     /locus_tag="OQQ48_001741"
     CDS             complement(5551..6744)
                     /gene="acrA"
                     /locus_tag="OQQ48_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HCS1952546.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDNKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTAPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            6886..7533
                     /gene="acrR"
                     /locus_tag="OQQ48_001742"
     CDS             6886..7533
                     /gene="acrR"
                     /locus_tag="OQQ48_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HCS1952547.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            7661..11023
                     /gene="mscK"
                     /locus_tag="OQQ48_001743"
     CDS             7661..11023
                     /gene="mscK"
                     /locus_tag="OQQ48_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HCS1952548.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLELQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIVGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYIDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(11235..11396)
                     /gene="rsmS"
                     /locus_tag="OQQ48_001744"
     CDS             complement(11235..11396)
                     /gene="rsmS"
                     /locus_tag="OQQ48_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HCS1952549.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(11410..11937)
                     /gene="priC"
                     /locus_tag="OQQ48_001745"
     CDS             complement(11410..11937)
                     /gene="priC"
                     /locus_tag="OQQ48_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HCS1952550.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRTT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            12007..12384
                     /locus_tag="OQQ48_001746"
     CDS             12007..12384
                     /locus_tag="OQQ48_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HCS1952551.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            12537..13088
                     /gene="apt"
                     /locus_tag="OQQ48_001747"
     CDS             12537..13088
                     /gene="apt"
                     /locus_tag="OQQ48_001747"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCS1952552.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            13217..15088
                     /gene="dnaX"
                     /locus_tag="OQQ48_001748"
     CDS             13217..15088
                     /gene="dnaX"
                     /locus_tag="OQQ48_001748"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCS1952553.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVPSALEKAP
                     AKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELAAKLAAEAIERDPWAARVS
                     QLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGAQQKLAEALSTLKGSTVEL
                     TIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQTLRRFFDAELDEESIRPI"
     misc_feature    14481..14545
                     /gene="dnaX"
                     /locus_tag="OQQ48_001748"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            15141..15470
                     /locus_tag="OQQ48_001749"
     CDS             15141..15470
                     /locus_tag="OQQ48_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCS1952554.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            15470..16075
                     /gene="recR"
                     /locus_tag="OQQ48_001750"
     CDS             15470..16075
                     /gene="recR"
                     /locus_tag="OQQ48_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCS1952555.1"
                     /translation="MQTSPLLTQLMEALRCLSGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            16185..18059
                     /gene="htpG"
                     /locus_tag="OQQ48_001751"
     CDS             16185..18059
                     /gene="htpG"
                     /locus_tag="OQQ48_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HCS1952556.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            18240..18884
                     /gene="adk"
                     /locus_tag="OQQ48_001752"
     CDS             18240..18884
                     /gene="adk"
                     /locus_tag="OQQ48_001752"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCS1952557.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            19016..19978
                     /gene="hemH"
                     /locus_tag="OQQ48_001753"
     CDS             19016..19978
                     /gene="hemH"
                     /locus_tag="OQQ48_001753"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HCS1952558.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTCEL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEYIEMMANLVAAYR"
     gene            complement(19975..20934)
                     /gene="aes"
                     /locus_tag="OQQ48_001754"
     CDS             complement(19975..20934)
                     /gene="aes"
                     /locus_tag="OQQ48_001754"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HCS1952559.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYPLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIVGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            21086..22390
                     /gene="gsk"
                     /locus_tag="OQQ48_001755"
     CDS             21086..22390
                     /gene="gsk"
                     /locus_tag="OQQ48_001755"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HCS1952560.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(22523..24199)
                     /locus_tag="OQQ48_001756"
     CDS             complement(22523..24199)
                     /locus_tag="OQQ48_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HCS1952561.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVAYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(24437..>24904)
                     /gene="fsr"
                     /locus_tag="OQQ48_001757"
     CDS             complement(24437..>24904)
                     /gene="fsr"
                     /locus_tag="OQQ48_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HCS1952562.1"
                     /translation="FYLMQKFGLSIQNAQLHLFAFLFAVAAGTVIGGPVGDKIGRKYV
                     IWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFSAILVYAQELLPGRIGMVSG
                     LFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLLGMLTIFLPDNRHKD"
BASE COUNT         6224 a         6490 c         6392 g         5798 t
ORIGIN      
        1 aacaaatggt agtgtccgct attgccagta cacctcacag cgcggcaggt acaaagcgcg
       61 tgttgtacca ttcgccgtta tagcaaacgc ggaaatccag cacgtcgata tcttcatagc
      121 ggtagcgtgg gtagacatcg aaaaagaaga aaccattaag caggataaat aatacaacca
      181 gtagccagac agagcctgac agcgtttcgg actgcaacat caccgccagg gtggcaaaaa
      241 aagcaacgta cataccgata aacagccccg gatgtttacg gataaaactg atactaaagc
      301 ggggtttgtt gtcgcgtttt tcgcgggtat tcagagactc aatcgtttcg gtcagcaggc
      361 gttgtatttc ggtcatcgtt tgccttcgtg gtatctgcaa ctttccaggg aacaccctat
      421 tttaacgtgg gaatagtaga ggggaagtaa cagatctacc acaattttgc ataacagttg
      481 cgaaaaaccg attacaattt tttaattatt ctggctggat taccgcccac gacaacgttg
      541 tccgggacac cttttgtgac aactgcacct gaagctacca cgacgttatc accaatggtc
      601 acaccagggt taatgaccgc gcgtccgcca atccagacgt tattaccgat ggtgacgggt
      661 ttccccagtt cagcaccgct attacgtgct acagggtcga tgggatgtgt tgccgtgtag
      721 atatgaacgc ctggtgccaa catacagtta tcaccgatgc gaatagggca gacatcaagc
      781 atcacgcaat cgaagttggc gaaaaaatta ttgccgagaa aaatgttata gccataatca
      841 cagcgaaacg ttggctcaat ataagcctct gtcacctgac cgaataaatc agcgagaatt
      901 tgctggcgta atgtgtgctc ttccgccagg gaatgattgt atcggtgaat aagctgacga
      961 gcgcgcaggc gatcgcgaga taacgtctca tctgccgagc gatacaactc accagcaatc
     1021 atcttttctt tttctgtgct cattactgac ctccggttgc tgaaaatgcg gcaacacagt
     1081 aagacgaaaa gtcagtgaac agaacgttcc cattacatct gtgtgataaa gatcacatag
     1141 ggtatgtctt catggtgaaa aagtataaaa ttcttaataa acagccggtt atagctccga
     1201 aagcgaatta tttattagcg aataaatttc catactgagg aagggatctt gtcgtatagt
     1261 ttattcatgg tcaattcggc gaggcggtga tctgcggctg agtaaaatac cgccagttca
     1321 ttatctgata attcgtattt atttttctcg ataacacgct ccagcgtgtc aattgtctgg
     1381 caacgacgta aacgcattaa ataatcggtt ttcgttaaag gtttttcgga catacttcta
     1441 cccatggttg taataattct aacaagataa actcgcagga ttctctttcg tcgcattgac
     1501 aaaacaacgg aatagtcgat tacctgactt ccgccatttc tgaagatcct gcatattaat
     1561 accataacta ctgaacaaca taaaggtgtc atccagatat tcgtcgatct gctcaatgag
     1621 cttattgtct tcattatact taattttgta attaagtgcg aaggtcgcaa tatgctcaat
     1681 cagttcattc aactggaggt tgatcgccga ggttgggtcg ttaacccagc cataattgct
     1741 ttcttcaagg tttgcaaggc agtcatgata cagggtttca cagagaaact taagctgtgc
     1801 gatatcatgt cttttgggtg agtattcatc cataacgcgt ccccttctta gcggttgaac
     1861 taacggacac ctttcgggat ggaaaaaact tactgacctg gacttgccct ctcgtttgtt
     1921 agcttaacta taagccactc tttgcaggta ttcattgcat tattacgaaa aattacaatt
     1981 aggaaggaat cgggacagga ctcacttttt catactagca ctaacccgca gcaggtaaaa
     2041 gcagtttgct cgaactgttc ataacatttc aggattatcg ccagatgaaa ttggtgacac
     2101 taataccagg attgctctga atatgacgta ataaccgagg aatgaataaa gaattaccgc
     2161 aaataattaa gaatagcctc taaatgatta tggattatta aatctattag cgctacttaa
     2221 tataaattaa ctaacaatca gtatttttta tgaattttat ccgtggttaa tactggtttt
     2281 cgtatgagat cctgagttgg tggttcaatt actccttaat gttcgtaggt tatgcataaa
     2341 aaaggccgct tgcgcggcct tagtgattac acgttgtatc aatgatgatc gacagtatgg
     2401 ctgtgctcga tatcttcatt cttgcggcta aagcggcggc gaaccaccac aaagaatacc
     2461 ggaacgaaga agattgccag taccgttgcg gtcaccatcc cgcccattac accggtacct
     2521 actgcgttct gcgcgccgga accagcacca gtactgataa ccagcggcat aacgccgagg
     2581 ataaacgcca gcgaggtcat caggatcgga cgtaaacgca tccgcactgc atcaagcgtc
     2641 gcttcaatca gacctttacc ttctttatcc atcaagtctt tggcgaattc gacgataagt
     2701 atcgcgttct tcgccgacaa cccaatggtt gtgagcaggc ctacttggaa gtaaacgtca
     2761 ttggtcaggc cacggaaggt ggcagccagc aacgcaccga taacccccag cggaacgacc
     2821 agcataacgg agaacggaat cgaccagctc tcgtacagcg ccgctagaca caggaatacg
     2881 acaatcaacg aaatcgcgta cagtgaaggt gcctggttgc cggagaggcg ttcctgatag
     2941 gacatccccg tccagtcata gccaacaccg gtaggcagtt tgctcgccag ttgttccatt
     3001 agctccattg cttcaccggt acttttaccc ggtgccgcct ggcctaagat ttccatggat
     3061 ggcaggccat tgtaacgttc cagacgcggc gaaccgtact cccaacgaga agaggagaat
     3121 gccgagaacg gcaccatctg accatcagca gcacgaacat accagtcgcc gatatcatcc
     3181 ggcagcatac ggtatttcgc ttctgacatg acgtaaactt tcttcacacg accgcggtcg
     3241 ataaagtcgt tcacatagct accgccccat gcagcgccca gagtggtgtt aatgtcgttg
     3301 atagaaacac ccagcgcctg cgctttttcc tggtcgatat caatcttaaa ctgcggggta
     3361 tcttccagac cgtttggacg tacgctggtc aacatatcag gatgctttgc tgcttctgca
     3421 agcagctggt tacgcgcctg agtcagtttt tcgtgaccaa ggccagcctg gtcaatcagc
     3481 tcaaagtcaa agccggttgc agtacccagt tccacgattg cgggtaggtt aaaggcgaaa
     3541 accatcgcat ctttgatttg cgagaaagcg cgtgttgcac gcatggtaat cgcttcaact
     3601 ttgttttctt cgcccggacg atcggcccag tccttcaagg aaacgaacgc aataccggta
     3661 ttctgaccac gtcccgcaaa gccgaagccg ttaacggcga acaccgactc aacgttgttc
     3721 ttttctttgg tcagatagta atgcgttacc tcattgagca ctttctgcgt acgttcctgc
     3781 gttgcacctg ctggcagctg aaccatggtc ataaatacgc cctggtcctc atctggcaag
     3841 aaggagcttg gcagacgcac gaacagatag gccatgccga ccacgatgat cagatacagc
     3901 accaggtaac gccccgtact gcgcagaata ccgcctacgc tgtcggtgta gtggtgcgtg
     3961 ctcttctcga acatgcggtt aaaccagccg aagaagcctt ttttaccttc cccgtgatcg
     4021 cctttggcaa tcggtttcag catggtggca caaagagccg gagtcaggat caacgctacc
     4081 agtaccgaca gcgccattgc tgaaacaatg gtaatagaga actgacgata gatagcacca
     4141 gtagaaccgc caaagaaggc catcggtacg aataccgctg acagtaccat cgcgataccg
     4201 accagagcgc cctgaatctg ccccatcgac ttacgggtag cttcttttgg cggcaaacct
     4261 tcttccgcca taacacgctc cacgttttct accacaacga tagcgtcatc caccaacaag
     4321 ccgatggcga gcaccatccc gaacattgtt agcgtgttta tcgagaagcc aaaggcggca
     4381 aggacggcaa aggtcccgag caataccacc ggtacggcaa tggtcggaat caacgtcgcg
     4441 cggaagttct gcaggaacag atacataacc aggaacacga ggatgatcgc ttcgaccagc
     4501 gttttaacca cttcgtgaat agagattttc acgaacggcg tggtgtcgta cgggtaaaca
     4561 attttcagac ccgacgggaa gaacggttcc atcttcgcca gttcagcacg gattgccgca
     4621 gcggtatcca gcgcatttgc accggtcgcc agcttgatcc ccagaccgga agccggttgg
     4681 ccgttaaact ctgcgatgat gtcgtagttc tcaccaccca gctcaatctt cgccacatca
     4741 cgcagcagga cgcgggaacc atcctgattc actttcagca ggattttgcc gaactcttca
     4801 gtagaggtca gacgcgtctg agcaataata gaggcgttaa gctgttggcc tttcaccggc
     4861 ggcgtaccac cgagctgacc cgccgcaacc tgggcgttct gcgctttgat ggcggtaatg
     4921 acatcaaccg gcgttagctg gaatttgttc agctcattcg ggttcatcca gatacgcatc
     4981 gcgtactgtg aaccgaacaa ctgaacatca cccacgcccg acgtacggct gatggcatct
     5041 ttcatattcg ccgctacgta gtcagagata tcctcctgcg tcatggtgcc atcggtgttg
     5101 ataacgccga caaccatcag gaagctgctg gatgatttct caacgctcac cccttgctgc
     5161 tgaacttctt gcggcagcaa cggcatcgcc agctgcagtt tgttctgcac ctgaacctgc
     5221 gcgatatccg catcagtacc agactcaaag gtcagggtga tctgcacggt ccccgtggag
     5281 tcactgttag aggacatgta catcaggtta tcgataccgt tcatattctg ttcgataacc
     5341 tgtgtcaccg tgtcctgcac tgttttcgca tcagcgccgg ggtaggaggc ggagatcgtt
     5401 actgccggcg gtgcaatcgt aggatattgc gccaccggca gtttgaggat cgccagcccc
     5461 cctgccaaca tgatgataat ggcgatcacc cacgcaaaaa tcgggcgatc gataaagaaa
     5521 ttaggcatgt cttaacggct cctgtttaag ttaagacttg gactgttcag gctgagcacc
     5581 gcttgcggct tgctggttat tatcagcggt aacttcttgt gcttttacct ggacaccagg
     5641 acgcactttc tgcagcccac ttattactac gcgatcgcct gctttcagac cttctgtcac
     5701 cagccactta tcgccaatag cctggcttgc aacgatcgga cgggtttcca ctttgtcatc
     5761 cgcgccaact accagtacgg tggcatcacc acgcggcgta cgggttacgc cctgttgcgg
     5821 gactaaaata gcgtttggat taagcccttc ttccagacgt gcgcgcacga acatacccgg
     5881 cagcagagtg tgatccgggt tcgggaagat agcgcgtagg gtgatagacc cagtggtctg
     5941 atcaacggta acgtcagaga attccagcgt accgtcctgc gggaacttaa tgccgtcact
     6001 ggtgatcagc gacactttgg ctttgccgtt ctcttgtttc agcgtgccat tcgccagttc
     6061 ctgtttcagg cgcaggaagt cgttgctgga ctgggtcaca tcaacgtaga tcggatcaag
     6121 ttgctgcacg gttgccagcg cagttgcctg accgttctgt accaatgcgc cttccgtcac
     6181 gttcgactta ccaatgcgac cgctaatcgg agcggtgact ttggtgtaag ccagattgat
     6241 ccgcgcagtt tcaacggcag ctttcgccgc agttaccgca gcattcgcct gttgcgcatc
     6301 agccagagcc tgatcgtact cttgcttact gatgtactga gtaccgagca gtttctgata
     6361 acgattcacc gtcaattgcg cgatattggc tgcagcctgg gctttcgcca gatcaccttt
     6421 cgcactgtcg tatgtcgcct gataggtcgc aggatcaatc tgatagagag agacaccagc
     6481 ttcgatgtcg ctaccttctt tgaaattacg cttcaggata atcccgctaa cttgaggacg
     6541 aacttctgcg atccggtagg cactggtgcg acccggaagc tcggttgtga tctgcagagg
     6601 ttcagttttg actgttacta cgccaacggc gggcatctgc tggccacctt gttgggcctg
     6661 tttgttgtca catcctgtta gggctaagct gcctgagagc atcagaacga ccgccagagg
     6721 cgtaaaccct ctgtttttgt tcatatgtaa acctcgagtg tccgatttca aattggtcaa
     6781 tggtcaaaag ttaataaacc cattgctgcg tttatattat cgtcgtgcta tggtacatac
     6841 attcacaaat gtatgtaaat ctaacgcctg taaattcacg aacatatggc acgaaaaacc
     6901 aaacaagaag cgcaagaaac gcgccaacac atcctcgatg tggctctacg tcttttctca
     6961 cagcaggggg tatcatccac ctcgctgggt gagattgcaa aagcagctgg cgttacgcgc
     7021 ggtgcaatct actggcattt taaagacaag tcggatttgt tcagtgagat ctgggaactg
     7081 tcagaatcca atattggtga actagagctt gagtatcagg caaaattccc tggcgatcca
     7141 ctctcagtat taagagagat attaattcat gttcttgaat ccacggtgac agaagaacga
     7201 cgtcgattat tgatggagat tatattccac aaatgcgaat ttgtcggaga aatggctgtt
     7261 gtgcaacagg cacaacgtaa tctctgtctg gaaagttatg accgtataga acaaacgtta
     7321 aaacattgta ttgaagcgaa aatgttgcct gcggatttaa tgacgcgtcg ggcagcaatt
     7381 attatgcgcg gctatatttc cggcctgatg gaaaactggc tctttgcccc gcaatctttt
     7441 gatcttaaaa aagaagcccg cgattacgtc gccatcttac tggagatgta tctcctgtgc
     7501 cccacgcttc gtaatcctgc cactaacgaa taaccctgaa tctgactcca ggatttttcc
     7561 tggacatttt cgtcgttgct attctggttc actgcgtcgt gatattcttg cggtttgact
     7621 ttttcaggtc gttcttcagg ttcagaaacc ttcattcatc atgactatgt tccagtatta
     7681 caaacgatca cggcattttg ttttttcagc atttattgct tttgtttttg tcttgttatg
     7741 ccagaacacg gcgtttgcgc gggcgtcatc gaatggtgat ctgccgacaa aagcggacct
     7801 gcaggcgcaa cttgactcac taaataaaca aaaagatctt tctgctcagg acaaactgga
     7861 gctgcaggat ctgacagata cattagccac cctcgataaa atcgatcgca taaaagaaga
     7921 gacagttcag ctacggcaaa aagtcgctga agcgccggaa aaaatgcgcc aggcgaccgc
     7981 ggcgttaaca gcacttagcg atgtcgataa cgacgaagaa acgcgcaaaa ttctgagtac
     8041 gctttcgttg cgccagctgg aaactcgcgt tgcccaggcg ctggacgatt tgcaaaacgc
     8101 acaaaacgat ctggcgtctt ataacagcca gctggtttcg ttacagacgc agcccgaacg
     8161 cgtgcaaaat gcgatgtata acgcttcgca gcagctgcaa caaattcgca gtcgtctgga
     8221 tgggactgat gtcggcgaga ctgccttacg tcccagccag aaagtgttaa tgcaggctca
     8281 gcaggcgttg ctgaatgcgg agattgacca gcagcgtaaa agcctggaag ggaacaccgt
     8341 cttgcaggat accttgcaaa agcaacgtga ttacgtgacg gcgaacagcg cccgtctgga
     8401 gcaccagttg cagctgttgc aagaagcggt aaacagcaag cgcctgactt taactgaaaa
     8461 aacggcgcag gaagccgtct ccccggatga agccgcgcgt attcaggcta atccgctggt
     8521 gaaacaggaa ctggaaatta accagcagtt aagtcagcgt ctgattaccg cgactgaaaa
     8581 cggtaatcag ttgatgcagc aaaacattaa agtcaaaaac tggctggagc gggcgctgca
     8641 atcggaacgc aatattaaag agcagattgc cgtcctgaag ggcagcctgc tgttgtctcg
     8701 tatcctttac cagcaacaac aaacgctgcc ctcggcggat gaactggaaa acatgaccaa
     8761 ccgcatcgcg gatttgcgtc tcgaacagtt tgaagttaac cagcagcgtg atgcactctt
     8821 ccagagcgat gcgttcgtca acaaactgga agaaggtcac accaacgaag tcaacagcga
     8881 agttcacgat gcgttattgc aagtggttga tatgcgtcgc gaattgctgg atcaactcaa
     8941 caaacagttg ggtaaccagc tgatgatggc cattaacctg caaatcaacc agcagcagtt
     9001 aatgagcgta tcgaaaaacc tgaaatccat cctgactcag caaatctttt gggtgaacag
     9061 taaccgtcca atggactggg actggatcaa agcgttcccg caaagcctga aagatgaatt
     9121 taagtcgatg aaaatcacgg tgaactggga aaaagcctgg cctgccgttt ttatcgcttt
     9181 cctcgccggt ttgccgctgc tgttgattgt cgggctgatc cactggcgtc tgggctggct
     9241 gaaagcgtat caacaaaaac tggcttccgc tgtgggttcc ctgcgtaacg atagccagct
     9301 caacacacca aaagcgatcc ttatcgacct gatccgcgcg ctgccggtgt gcctgattat
     9361 tctcgcggtt ggcttgattc tgttgaccat gcagctcaac atcagcgaac tgctatggtc
     9421 gttcagcaaa aaactggcga ttttctggct ggtgtttggc ctgtgctgga aggtactgga
     9481 gaagaacggc gttgccgtgc gtcacttcgg catgccggaa cagcagacca gccactggcg
     9541 tcggcaaatt gtccgcatca gtctcgcatt gctgcctatc catttctggt ctgtggtggc
     9601 agaactttcc ccgctgcatc tgatggatga tgtgctgggg caagcgatga ttttcttcaa
     9661 cctgctgctg attgccttcc tggtatggcc aatgtgccgc gaaagctggc gtgataaaga
     9721 gtcgcacacc atgcgactgg tcaccattac cgtgctgtcg attatcccga ttgcattgat
     9781 ggtgctgact gccacgggtt atttctacac cacgctgcgc ctctccgggc gttggattga
     9841 aaccgtttat ctggtgatca tctggaactt gctctaccag acggtattgc gcggcttaag
     9901 cgtagcggcg cggcgtatcg cctggcgtcg tgcgctggcg cgtcggcaga atctggtgaa
     9961 agagggcgca gaaggtgccg aaccgccgga agaacccacc attgcactgg aacaggttaa
    10021 ccagcagacg ctgcgtatta ccatgttgct gatgtttgcg ctgttcggtg tcatgttctg
    10081 ggcaatttgg tccgatttga tcaccgtgtt cagctatatc gacagcatta cgctctggca
    10141 ttacaacggt actgaagctg gcgctgcggt ggtgaaaaac gtcaccatgg gcagtctgtt
    10201 gtttgcgatt atcgcctcaa tggtggcctg ggcgttgatt cgcaacctgc ctggtttgct
    10261 ggaagtgctg gtgctctcgc gactgaatat gcgccagggc gcgtcgtatg ccattactac
    10321 catccttaac tacatcatta ttgccgttgg tgcgatgacg gtgttcggat cgctgggcgt
    10381 ctcttgggat aaactccagt ggctggcggc agcattatcc gtaggtcttg gttttggttt
    10441 acaagaaatt ttcggtaact tcgtctccgg tttgatcatt ctgttcgaac gtccggtgcg
    10501 tattggcgat acggtaacca ttggtagctt ctcggggacg gtaagtaaga tccgtattcg
    10561 tgcgacaacg attaccgatt tcgatcgcaa agaagtgatc atcccgaaca aagcgtttgt
    10621 taccgagcgt ctgatcaact ggtcgttgac tgacactact acgcgtctgg tgatccgtct
    10681 cggcgtggcc tatggctccg atctggaaaa agtgcgtaaa gtgttactga aggcggcgac
    10741 tgagcaccca agggtgatgc acgaaccaat gccggaagtc ttctttacgg cgtttggtgc
    10801 cagcacgttg gatcatgagc tgcgtctgta tgtgcgtgaa ctgcgtgacc gtagtcgtac
    10861 tgtcgatgag ctgaaccgta ctatcgatca gctgtgccgt gaaaacgaca tcaacattgc
    10921 ctttaaccag cttgaagtgc atctgcacaa cgagaagggc gatgaggtga cggaagtaaa
    10981 acgcgactac aaaggcgatg acccgacgcc agcggtaggg taataaccaa agggggcgac
    11041 atttagttgc cccgagatta ctgacaaagt gcgcgttgtt catgccggat gcggcgtgac
    11101 cgccttatcc ggcctacgaa accgcaagaa ttcaatgtat tgcaggagcg gtgtaggcct
    11161 gataagcgta gcgcatcagg cagttttgcg tttgcccaca atcttagggg acatttagcg
    11221 gccccattta tttctcactt ttccgcctca tcatcgcgcg ttaatttctt ttcataatcg
    11281 cgttttacaa tatccagcgc gcgcagcacg gtgcgggctg ggatctgatt ttcttccagc
    11341 agcacaatca aatcgacagc cagtttgaca tcgtcagggg cattttccag tgacatactc
    11401 tctccattgc tagcgggtta agcgcgctag cctgttttcg attttttcca gcgcatggcg
    11461 gcagcgtgcc aggcgcgctt cataggcttc cacttcacga tgcagcgttt gctgttccac
    11521 gagatcggtc acccgcgcca ggcgggctct gcgttcggca accatctcac gtagccgccg
    11581 ctcaaaatcc tgatgctgaa tacgtttacg ctgccagcgg gcaattttcg gtggcgcact
    11641 gtcccactcg cgcaatgacc aggcggaggc ttcacgcgcg atggcttcca gttgtgccgc
    11701 cagatgttcc gccagccagg ccacttgcgg cagctgttgc tgctcaactg catgacgaag
    11761 cgcagccaga ttatcgccag cctcatcgag acaagcctgc agtgttgtcg tacgggtttg
    11821 aaaaaggtgt ctgtcaaaac gggcacttag cgtggcaaat tgcgctaccg gggcacaacg
    11881 ctgacgcagc gtagcgagct gtccttccag tttttccagc agcagggcgg ttttcacgat
    11941 aagacctcaa tgaaaatgat aattgttatg ctaaagtagc cactctgtaa gctgacaaca
    12001 ctcaatatgc aacgaatcat tttaatcatc attggctggc tggcggtagt gctgggtacg
    12061 ctgggcgtgg tattaccggt attaccgacg acgccgttta tcctgctggc ggcctggtgc
    12121 tttgcccgtt cttccccgcg ctttcacgcc tggttgctgt accgctcatg gtttggcagc
    12181 tatctacgtt tctggcagaa acatcatgcg atgccgcgcg gcgtcaaacc gcgggcgatt
    12241 ttgcttattt tgctcacgtt tgccatttct ctgtggttcg tccagatgcc atgggtgcgc
    12301 atcatgttgc tggtaattct cgcctgtttg cttttctata tgtggcgaat tccggtgatt
    12361 gatgaaaagc aagaaaagca ctgaagcaca acaatcgcag ttgcaattat tgcgtacagc
    12421 cagtacattc tggcgttttc gagcacaggc gcaggcggtc aaaggttaaa caactgttac
    12481 ttttgatacg tttaaaacgc gccgtgagta ccaccgtaac aagcaggcat acacttatga
    12541 ccgcgactgc acagcagctt gagtatctca aaaatagcat caaaagcatt caggactacc
    12601 caaaacccgg cattcttttc cgcgatgtca ccagcttact ggaagacccg aaagcttacg
    12661 ctctcagcat cgacttgctg gttgagcgtt acaaaaatgc gggcattacc aaagttgtcg
    12721 gcaccgaagc gcgtggcttc ttgtttggcg ctccggtagc tctgggtctg ggcgttggct
    12781 ttgtaccggt ccgtaaaccg ggcaaactgc cgcgtgaaac catcagtgaa acttacgacc
    12841 tggaatacgg caccgatcag ctggagatcc acgttgatgc catcaaaccg ggcgacaaag
    12901 ttctggtggt ggacgatctg ctggcaaccg gcggcactat cgaagcgacc gttaaactga
    12961 tccgtcgtct gggtggtgaa gtggctgacg ctgcgttcat tatcaacctg ttcgatctcg
    13021 gcggcgaaca gcgtctcgaa aaacagggca ttaccagcta cagccttgtc ccgttcccgg
    13081 gccattaatt atcgccagtc ttgtgctgcc cacgctacgg acagcacaag atgtgcattc
    13141 agcctcgccg ttctgacggg gctgtgttag cattacccct tcgtgaatcc accttccagc
    13201 gtttcagagc ctgccaatga gttatcaggt cttagcccga aaatggcgcc cacaaacctt
    13261 tgctgacgtc gtcggccagg aacatgtgct gaccgcactg gcgaacggct tgtcgttagg
    13321 gcgtattcat catgcttatc ttttttccgg tacccgtggc gtcggaaaaa cctctatcgc
    13381 ccgactgctg gcgaaggggc taaactgcga aaccggcatt accgcgacgc cgtgcggcgt
    13441 gtgcgataac tgtcgtgaaa tcgagcaggg gcgctttgtc gatctgattg aaatcgacgc
    13501 cgcctcgcgc accaaagttg aagatacccg cgacctgctg gataacgtcc agtacgctcc
    13561 ggcgcgtggt cgtttcaaag tttatctgat cgacgaagtg catatgctgt cgcgccacag
    13621 ctttaacgca ctgttaaaaa cccttgaaga gccgccggag cacgttaagt ttctgctggc
    13681 gacgaccgat ccacagaaat tgccggtgac gattttgtcg cgctgtttgc aatttcatct
    13741 caaggcgctg gatgtcgagc aaattcgcca tcaacttgag cacatcctca acgaagaaca
    13801 tatcgctcac gagccgcggg cgttgcaatt gctggcgcgc gccgctgaag gcagcctgcg
    13861 agatgcctta agtctgaccg accaggcgat tgccagcggt gacggccagg tttcaaccca
    13921 ggcagtcagt gcgatgctgg gtacgcttga tgacgatcag gcgctgtcgc tggttgaagc
    13981 gatggtcgag gccaacggcg agcgcgtaat ggcgctgatt aatgaagccg ctgcccgtgg
    14041 tatcgagtgg gaagcgttgc tggtggaaat gctcggcctg ttgcatcgta ttgcgatggt
    14101 acaactttcg cctgctgcac ttggcaacga catggccgcc attgagctgc ggatgcgtga
    14161 actggcgcgc accataccgc cgacggatat tcagctttac tatcagacgc tgttgattgg
    14221 tcgcaaagaa ttaccgtatg cgccggaccg ccgcatgggc gttgagatga cgctgctgcg
    14281 cgcgctggcg ttccatccac gtatgcctct gcctgagcca gaagtgccgc gccagtcctt
    14341 tgcacccgtc gcgccaacgg cagtaatgac gccaacccag gtgccgccgc aaccgcaatc
    14401 agcgccgcag caggcaccga ctgtaccgct cccggaaacc accagccagg tgctggcggc
    14461 gcgccagcag ttgcagcgcg tgcagggagc aaccaaagca aaaaagagtg aaccggcagc
    14521 cgctacccgc gcgcgtccgg tgccatcggc gctggaaaaa gcgccagcca aaaaagaagc
    14581 gtatcgctgg aaggcgacca ctccggtgat gcagcaaaaa gaagtggtcg ccacgccgaa
    14641 ggcgctgaaa aaagcgctgg aacatgaaaa aacgccggaa ctggcggcga agctagcggc
    14701 agaagccatt gagcgcgacc cgtgggcggc acgggtgagt caactttcgc taccaaaact
    14761 ggtcgaacag gtggccttaa atgcctggaa agaggagagc gacaacgcag tatgtctgca
    14821 tttgcgctcc tctcagcggc atttgaacaa ccgcggtgca cagcaaaaac tggctgaagc
    14881 gttgagcacg ttaaaaggtt caacggttga actgactatc gttgaagatg ataatcccgc
    14941 ggtgcgtacg ccgctggaat ggcgtcaggc gatatacgaa gaaaaacttg cgcaggcgcg
    15001 cgagtccatt attgcggata ataatattca gaccctgcgt cggttcttcg atgcggagct
    15061 ggatgaagaa agtatccgcc ccatttgatc gtaagcacag cttacgttcg tcatccttaa
    15121 cgtgattgag agagaaacct atgtttggta aaggcggtct gggtaacctg atgaagcaag
    15181 cccagcagat gcaagaaaaa atgcagaaaa tgcaggaaga gatcgcgcag ctggaagtca
    15241 ccggcgaatc tggcgcaggt ctggtaaaag tgaccatcaa cggtgcacac aactgccgtc
    15301 gcgtagagat cgacccgagc ctgctggaag acgacaaaga gatgctggaa gacctggtgg
    15361 ctgcagcatt caacgacgca gcacgtcgta ttgaagaaac gcagaaagaa aaaatggcct
    15421 ctgtatcctc cggaatgcag ctgccgcctg gctttaagat gccgttctga tgcaaaccag
    15481 cccgctgtta acacagctta tggaagcact gcgctgtctg tcgggcgttg gcccgaagtc
    15541 ggcgcagcgt atggcgttca cgctgcttca gcgcgatcgt agcggcggga tgcgtctggc
    15601 gcaggcgctc acccgggcga tgtcggaaat cggccactgc gccgattgcc gcactttcac
    15661 cgaacaggaa gtctgtaaca tctgttcgaa tccgcgtcgt caggaaaacg gtcaaatctg
    15721 tgtggtggag agtccggcgg acatctacgc cattgagcag acggggcagt tttctggtcg
    15781 ttattttgtg ttgatggggc atctgtcacc gctggacggc atcggtccgg atgatatcgg
    15841 gcttgatcgt ctggaacagc gtctggcaga ggaaaaaatc actgaagtga tcctcgccac
    15901 caatcccacg gttgaaggtg aagctaccgc taactacatt gccgagcttt gcgcgcaata
    15961 tgacgtggaa gctagccgaa tcgctcatgg cgtaccggtt ggcggcgaac tggaaatggt
    16021 cgatggcacc acgctgtcac actcccttgc cgggcgtcat aagattcgtt tttaagcaaa
    16081 cgagagcagg atcacctgct ctcgcttgaa attattctcc cttgtcccca tctctccctc
    16141 atcctgtttt taaccttaaa atggcattat tgaggtagac ctacatgaaa ggacaagaaa
    16201 ctcgtggttt tcagtcagaa gtgaaacagc ttctgcacct gatgatccat tctctctatt
    16261 ccaataaaga aatcttcctg cgtgagctta tctctaacgc ctccgatgcg gcggacaagc
    16321 tgcgtttccg tgcgctatct aacccggacc tgtacgaagg tgatggcgaa ctgcgcgttc
    16381 gtgtctcttt cgataaagac aagcgcacgc tgaccatctc cgataacggc gtggggatga
    16441 cccgcgacga agtgattgac catctgggga ctatcgctaa atccggtacc aaatcattcc
    16501 tcgaatccct gggttctgac caggcgaaag acagccagct gatcggtcag tttggtgttg
    16561 gtttctactc tgcgtttatc gtggccgaca aagtgactgt gcgtactcgc gcggcaggcg
    16621 aaaaaccaga aaacggcgtc ttctgggaat cggctggcga aggtgaatat accgttgccg
    16681 acatcaccaa agaagatcgt ggtactgaaa tcaccctgca tctgcgtgaa ggcgaagacg
    16741 agttcctcga tgactggcgc gtgcgttcca tcatcagcaa atactccgac catatcgcgc
    16801 tgccggtaga gatcgaaaaa cgcgaagaga aagacggcga aaccgttatc tcctgggaga
    16861 aaatcaacaa agcgcaggcg ctgtggactc gtaacaagtc ggaaatcacc gatgaagagt
    16921 acaaagagtt ctacaaacac atcgcccacg actttaatga tccgctgacc tggagccaca
    16981 accgcgttga aggtaagcag gagtacacca gcctgctgta catcccgtcc caggctccgt
    17041 gggatatgtg gaaccgtgat cataaacacg gcctgaaact gtacgtccag cgtgtgttca
    17101 tcatggacga cgcagaacag ttcatgccga actatctgcg cttcgtgcgt ggtctgattg
    17161 actccagcga tctgccgctg aacgtttccc gtgaaatcct ccaggacagc acggtaacgc
    17221 gtaacctgcg caatgcgctg accaagcgtg tgctgcaaat gctggaaaaa ctggcgaaag
    17281 acgacgcgga aaaataccag accttctggc aacagtttgg cctggtactg aaagaaggtc
    17341 cggcggaaga tttcgctaac caggaagcga tcgccaaact gctgcgtttt gcttctaccc
    17401 ataccgattc ttctgcgcag accgtatctc tggaagacta cgtttcccgc atgaaagaag
    17461 ggcaggagaa aatctactac atcaccgcag acagctatgc ggcagcgaag agcagcccgc
    17521 acctggaact gctgcgtaag aaaggcatcg aagttctgct gctttccgac cgcatcgatg
    17581 agtggatgat gaactatctg actgagttcg acggtaaacc gttccagtcg gtgtctaaag
    17641 ttgacgagtc gctggaaaaa ctggctgacg aagttgatga gagcgcgaaa gaagcggaga
    17701 aagcactgac tccgttcatc gaccgtgtga aagccttgct cggcgagcgc gtgaaagatg
    17761 tccgtctgac tcaccgtctg accgatacgc cagcgatcgt ttcgaccgac gcggacgaaa
    17821 tgagcactca gatggcgaaa ctgttcgctg cggcgggcca gaaagtgcca gaagtgaaat
    17881 acatcttcga actgaacccg gatcacgtac tggtgaaacg tgcggcagat actgaagatg
    17941 aagcgaagtt cagcgagtgg gtagaactgc tgctggatca ggcgctgctg gcagaacgcg
    18001 gcacgctgga agatccgaac ctgtttattc gtcgtatgaa ccagctgctg gtttcctgat
    18061 gtcatgccgg atgacctttg tgtcatccgg catttttctt ttcatcatct gcactttccg
    18121 caaattatct cgccattaac cgtttcagcc tcaggtgcct ttcttgaggc aatcgcctgt
    18181 tggtggtatc gtttatcgct ttttcaaaaa attcgacaca ttttaagggg attttcgcaa
    18241 tgcgtatcat tctgcttggc gctccgggcg cggggaaagg gactcaggct cagttcatca
    18301 tggagaaata tggtattccg caaatctcca ctggcgatat gctgcgtgct gcggtcaaat
    18361 ctggctccga gctgggtaaa caagcaaaag acattatgga tgctggcaaa ctggtcaccg
    18421 acgaactggt gatcgcgctg gttaaagagc gcattgctca ggaagactgc cgtaatggtt
    18481 tcctgttgga cggcttcccg cgtaccattc cgcaggcaga cgcgatgaaa gaagcgggca
    18541 tcaatgttga ttacgttctg gaattcgacg taccggacga actgattgtt gatcgtatcg
    18601 taggccgccg cgttcatgcg ccgtctggtc gtgtttatca cgttaaattc aatccgccga
    18661 aagtagaagg caaagacgac gttaccggtg aagaactgac tacccgtaaa gacgatcagg
    18721 aagaaaccgt acgtaaacgt ctggttgaat accatcagat gactgcaccg ctgatcggct
    18781 actactccaa agaagcggaa gcgggtaaca ccaaatacgc gaaagttgac ggcaccaagc
    18841 cggttgctga agttcgcgct gatctggaaa aaatcctcgg ctaatcaagc cgcagcagat
    18901 ctgctttcgg gcaggtctgc tcatccacct caggcaatac ctctcacagc aattagttct
    18961 tcttcctcac ttttccgcta caattatcaa caagttgaat cgataagagg cggtaatgcg
    19021 tcagactaaa accggtatcc tgctggcaaa cctgggtacg cccgatgccc ccacacctga
    19081 agcggtaaaa cgctatctga aacaattttt aagcgacaga cgcgtggttg atacctcacg
    19141 gttgttatgg tggccattgc tgcgcggcgt tattttgccg ctgcgctcgc cgcgtgtggc
    19201 gaagctgtat gcctctgtct ggatggaagg tggctcgccg ctgatggttt acagccgcca
    19261 gcaacagcag gcgctggcac aacgtttacc ggagacgcct gtagcgctgg gaatgagcta
    19321 cggctcgcca tcactggaaa gcgccgtcga tgaactcctg gcagagcatg tagatcatat
    19381 tgtggtgctg ccgctttatc cgcaattctc ctgttctacg gtcggtgcgg tatgggatga
    19441 actggcacgc attctggcgc gcaaacgtag cattccgggg atatcgttta ttcgtgatta
    19501 cgccgataac cacgattaca ttaatgcact ggcgaacagc gtacgcgctt cttttgccaa
    19561 acatggcgaa ccggatctgc tgctgctctc ttatcatggc attccccagc gttatgcaga
    19621 tgaaggcgat gattacccgc aacgttgccg cacaacgact tgcgaactgg cttccgcact
    19681 ggggatggca ccggaaaaag tgatgatgac ctttcagtcg cgctttggcc gggaaccctg
    19741 gctgatgcct tataccgacg aaacgctgaa aatgctcgga gaaaaaggcg taggtcatat
    19801 acaggtgatg tgcccgggct ttgctgcgga ttgtctggag acgctggaag agattgccga
    19861 gcaaaaccgt gaggtcttcc tcggtgccgg cgggaaaaaa tatgaatata ttccagcgct
    19921 taatgccacg ccagaatata tcgaaatgat ggctaatctt gttgccgcgt atcgctaaag
    19981 ctgagcggta aagaactgag cgccgtcgcg aagagcctcg tcggcggttt tcatcattcg
    20041 tgaataatgc aaaaaggcat gcagcgtacc tgggtagagt ttgaactcac agggctgctg
    20101 atgcgccgct aacgtctggt aaagcagacg gctgtcatcc agcagcggat cgaactccgc
    20161 cccggcaata aaacagggcg gaacttcgcg agtgagatca ttattaaaca gacagtaata
    20221 cggtgactcg cggtccgcgt cgttgcttaa atacgcctct tcatacatct gcaaatcctg
    20281 ttgcgttaag ccatcccaga caccgcccaa cagacgacga gtcacggaat cccgtaatcc
    20341 gtaaagccca taccacagca aaacacccac aattttaccg caatcgatct gtttatcacg
    20401 caaccacaac gcactggcga gcgccagcat ggcacctgcg gaatcaccgg caaagccaat
    20461 gcgggacata ttgatttgat aatcctccgc ctgctggtgg aaataacaac aagcagccac
    20521 aatttcctct atcgcttgcg gaaaacgcgc ttcaggtgaa agggggtaat caataccaat
    20581 caccgtacat tggctgtagc ttgccagcag gcgcatgatg cgatcgtggg tatcgagatt
    20641 gccgagaata aaaccgcctc catgcaaata aaatagcgtc gccgggctat ctggctgcgg
    20701 acaaaagaga cgtgtttcca cctgcccata ttttgttgga accatgtaag ctctggttgc
    20761 catttctgga gcgcccgcat tccagaatcg gcgctcaagc gtgtaatact gtcgttgctc
    20821 agcaatcgtt cccgttgcgg gccaggacgg taaatccggc tgaagagtat tcacaacggt
    20881 cttcatttca gcagaaataa ggtccagaac aggtagtttg ttttccggct tcatacaaaa
    20941 actcctttca aattacgtca ttgtaaggaa ccactgccat gaaaatgcga tcccgcctgc
    21001 tgatattgaa actggctgcg tctcgcgcgc tcccgtcaga ttgtgttaac attcgccgct
    21061 cagttaacca cccgtaaaaa caaccatgaa atttcccggt aaacgtaaat ccaaacatta
    21121 cttccctgta aatgcacgcg atccgctgct tcagcaattc cagccagaaa acgaaaccag
    21181 cgccgcctgg gtcgtaggta ttgaccagac gctggtcgat attgaagcga aagtggatga
    21241 tgaattcatt gagcgttatg gattaagcgc cgggcattca ctggtgattg aggatgacgt
    21301 agccgaagcg ctttatcagg agctaaaaca gaaaaacctg attacccatc agtttgcggg
    21361 tggcaccatt ggtaacacca tgcacaacta ctcggtgctc gcggacgacc gttcggtgct
    21421 gctgggcgtc atgtgcagca atattgaaat tggcagttat gcctatcgtt acctgtgtaa
    21481 cacttccagc cgtaccgatc ttaactatct acaaggcgtg gatggcccga ttggtcgttg
    21541 ctttacgctg attggcgagt ccggggaacg tacctttgct atcagtccag gccacatgaa
    21601 ccagctgcgg gctgaaagca ttccggaaga tgtgattgcc ggagcctcgg cactggttct
    21661 cacctcttat ctggtgcgtt gcaaaccggg tgaacccatg ccggaagcaa ccatgaaagc
    21721 cattgagtac gcgaagaaat ataacgtacc ggtggtgctg acgctgggca ccaagtttgt
    21781 cattgccgag aatccgcagt ggtggcagca attcctcaaa gatcacgtct ctatccttgc
    21841 gatgaacgaa gatgaagccg aagcgttgac cggagaaagc gatccgttgt tggcatctga
    21901 caaggcgcta gactgggtag atctggtgct gtgcaccgcc gggccaatcg gcttgtatat
    21961 ggcgggcttt accgaagacg aagcgaaacg taaaacccag catccgctgc tgccgggcgc
    22021 tatagcggaa ttcaaccagt atgagtttag ccgcgccatg cgccacaagg attgccagaa
    22081 tccgctgcgt gtatattcgc acattgcgcc gtacatgggc gggccggaaa aaatcatgaa
    22141 cactaatgga gcgggggatg gcgcattggc agcgttgctg catgacatta ccgccaacag
    22201 ctaccatcgt agcaacgtac caaactccag caaacataaa ttcacctggt taacttattc
    22261 atcgttagcg caggtgtgta aatatgctaa ccgtgtgagc tatcaggtac tgaaccagca
    22321 ttcacctcgt ttaacgcgcg gcttgccgga gcgtgaagac agcctggaag agtcttactg
    22381 ggatcgttaa gttatcgtcg gttcgtaggc cagataaggc gttcacgccg catctggcat
    22441 ttggctctcg atgcctgatg cgacgctggc gcgtcttatc atgcctacat atttttcata
    22501 ttttacatcc ggcaaccacc gtttaccccg tcaccacctc acccgccggt ggcgtttcca
    22561 gcagttccag catggtacgg gcgatttcac gctcgcccat cactacctga ttcgcaccac
    22621 gttcggtgat atacgccact tcatcgtcat aatgggcgcg ggcaataatc tcaatatccg
    22681 gatttttcgc gcgggcagat gccacaatct cacccgcttc ataaccgttg ggaatcgtca
    22741 ggatcagcca ttttgcacat tccagatgcg ccagttgcat aatttcttcg ttcgccgcat
    22801 tgcccaatac tgcgcggacc ccgcgctctc gcagctcatc aacacgggtt cgtgacgtct
    22861 caatcaccac cagcggaata tcagaggcga gcaatttctc ccccagcagg ctgcctacac
    22921 gaccgtaacc caccagtagc gcatggttgc aaatatccac tgggatctgc ttctcttctt
    22981 cgattgcctc ttccagcgtc tgctcttcca gcgtttcggt cttcgccaga tatttctcca
    23041 gtagtgcgaa cagtaccggg ttgagcataa tcgacaggat cgcccctgcc agtaccaggt
    23101 tttgtccggc ctgcggcagt aaattcaatg ccattcccag tcccgccagg ataaacgcga
    23161 actcaccaat ctgcgccagg ctggcggcga tggttaatgc cgtacgttgg gagtgaccaa
    23221 acagtcgcac caggaaaaat gcggctaacg acttaccaaa cagaataatc gccagcgtcg
    23281 ccagcactgc cagcggttgc tgaatcagaa ttaacggatc aaacaacatc ccgacggaga
    23341 caaaaaacag caccgcaaac gcgtcgcgca atggcagcgt atcgtgggcg gcacggtgac
    23401 tcagttcaga ctcgttcagt accatcccgg caaagaacgc accgagtgca aaggagacat
    23461 caaacagctc taccgcacca aaggcaatcc ctaacgccag cgccagcacc gacagggtaa
    23521 acagctcgcg agaaccggtt gccgcgctgc gtgccataat ccacggcacc agacggcgac
    23581 ctaccagcat cataatggcg ataaatgcga tcactttgcc gatggtgatc cccatatcga
    23641 ctgcaagagt ggcaaagccc acatcgccct gttccatcat tcctgccact gcgggcagca
    23701 acaccagcgt cagaaccatt accaggtctt ccacaatcaa ccaaccgatg gcgatttgcc
    23761 cacgctgact gtcaattaat tgccgttctt caagtgcgcg cagtaacacc acggtactgg
    23821 cggtggaaag acataaaccg aacacgatac cggtcattaa cgaccagccc agcacggcag
    23881 agagcgccat acccagcagc gtcgccacgg ctatctgggc gatcgcaccg ggaatggcga
    23941 tggcctttac cgccatcaaa tccttcagcg aaaagtgcaa accgacgcca aacatcaaca
    24001 gaatgacgcc cagttcagcc agttccggcg caagcttggt atcggcaaca aagcccggag
    24061 tgaatggtcc tgccagcaca cccgctaaca gatatcccac cagaggagaa atacgtagtt
    24121 tattggccag catgccgagg ataaaggcga gcacaaggcc gccaacaatg gtggtgataa
    24181 gcggggtggc gtgatgcatt ccgtctcctt ttcctggtgg ttattgtcca tttttggccg
    24241 ggaaaaccaa aattacaggt aatagtttat gacaatttca ttgatgatgt tcatgaataa
    24301 ttattgaatt ttgcagaaaa atggaattag ctgcaaaaaa agcacggatc ggaaaacaga
    24361 aggcggttta acagaggaaa ggcttatggc gactgtaaaa cgatgcagcc aaagtttgcc
    24421 tttggctgca atgaaatcag tctttatgcc ggttatcagg caggaatatg gtcaacatcc
    24481 ccaatagtgg caggaaagca cagattttat agactaactc gatgctggtg tgatcggcga
    24541 taagccccag aactgccgct cccagacctc ccatgccaaa agcaaaaccg aaaaagagtc
    24601 cagaaaccat accgatacgt cccggaagta gctcctgagc gtagaccaga atggcagaga
    24661 atgccgaagc gaggataaat ccaataatca ccgttaaaac ccccgtccag tgcaggctgg
    24721 cgtagggtaa aatcagcgta aacggcgcaa cgccgaggat agagccccaa atcacatatt
    24781 tccgtccaat tttatcccct acaggcccgc cgatcaccgt acctgccgca acggcaaaca
    24841 ggaaggcaaa cagatgaagc tgggcattct ggatagataa tccgaatttt tgcatcagat
    24901 aaaa
//
LOCUS       DAJVUJ010000047        24756 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.047, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000047 DAJVUJ010000000
VERSION     DAJVUJ010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24756
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.047"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(<1..224)
                     /locus_tag="OQQ48_001758"
     CDS             complement(<1..224)
                     /locus_tag="OQQ48_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HCS1952563.1"
                     /translation="MTWNLLALATALQTVPEQNIDVTNSENALIIKMNDYGDLQINIL
                     FTSRQMIIETFICPVSSISNPDEFNTFLLR"
     gene            complement(351..1082)
                     /gene="rlmB"
                     /locus_tag="OQQ48_001759"
     CDS             complement(351..1082)
                     /gene="rlmB"
                     /locus_tag="OQQ48_001759"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCS1952564.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     AQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLVRT
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(1263..3704)
                     /gene="rnr"
                     /locus_tag="OQQ48_001760"
     CDS             complement(1263..3704)
                     /gene="rnr"
                     /locus_tag="OQQ48_001760"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCS1952565.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAECRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRLVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3743..4168)
                     /gene="nsrR"
                     /locus_tag="OQQ48_001761"
     CDS             complement(3743..4168)
                     /gene="nsrR"
                     /locus_tag="OQQ48_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HCS1952566.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4373..5671)
                     /gene="purA"
                     /locus_tag="OQQ48_001762"
     CDS             complement(4373..5671)
                     /gene="purA"
                     /locus_tag="OQQ48_001762"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCS1952567.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5775..5972)
                     /locus_tag="OQQ48_001763"
     CDS             complement(5775..5972)
                     /locus_tag="OQQ48_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HCS1952568.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(6054..7058)
                     /gene="hflC"
                     /locus_tag="OQQ48_001764"
     CDS             complement(6054..7058)
                     /gene="hflC"
                     /locus_tag="OQQ48_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HCS1952569.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(7061..8320)
                     /gene="hflK"
                     /locus_tag="OQQ48_001765"
     CDS             complement(7061..8320)
                     /gene="hflK"
                     /locus_tag="OQQ48_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HCS1952570.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTYPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8406..9686)
                     /gene="hflX"
                     /locus_tag="OQQ48_001766"
     CDS             complement(8406..9686)
                     /gene="hflX"
                     /locus_tag="OQQ48_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCS1952571.1"
                     /translation="MFDRYDAGEQAVLVHIYFTKDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9763..10071)
                     /gene="hfq"
                     /locus_tag="OQQ48_001767"
     CDS             complement(9763..10071)
                     /gene="hfq"
                     /locus_tag="OQQ48_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HCS1952572.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(10157..11107)
                     /gene="miaA"
                     /locus_tag="OQQ48_001768"
     CDS             complement(10157..11107)
                     /gene="miaA"
                     /locus_tag="OQQ48_001768"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCS1952573.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(11100..12947)
                     /gene="mutL"
                     /locus_tag="OQQ48_001769"
     CDS             complement(11100..12947)
                     /gene="mutL"
                     /locus_tag="OQQ48_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCS1952574.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRSIPENRVAAGRNHFAEPAAREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKLKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQVQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNVM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12957..14294)
                     /gene="amiB"
                     /locus_tag="OQQ48_001770"
     CDS             complement(12957..14294)
                     /gene="amiB"
                     /locus_tag="OQQ48_001770"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HCS1952575.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKHSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14313..14774)
                     /gene="tsaE"
                     /locus_tag="OQQ48_001771"
     CDS             complement(14313..14774)
                     /gene="tsaE"
                     /locus_tag="OQQ48_001771"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCS1952576.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14746..16293)
                     /gene="nnr"
                     /locus_tag="OQQ48_001772"
     CDS             complement(14746..16293)
                     /gene="nnr"
                     /locus_tag="OQQ48_001772"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HCS1952577.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLFAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLRQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            16292..17431
                     /gene="queG"
                     /locus_tag="OQQ48_001773"
     CDS             16292..17431
                     /gene="queG"
                     /locus_tag="OQQ48_001773"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCS1952578.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQPICPWNRYSQLTTEEDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17414..17467)
                     /locus_tag="OQQ48_001774"
     CDS             complement(17414..17467)
                     /locus_tag="OQQ48_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1952579.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(17696..17771)
                     /locus_tag="OQQ48_001775"
     tRNA            complement(17696..17771)
                     /locus_tag="OQQ48_001775"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17736..17738),aa:Gly,seq:gcc)
     gene            complement(17808..17883)
                     /locus_tag="OQQ48_001776"
     tRNA            complement(17808..17883)
                     /locus_tag="OQQ48_001776"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17848..17850),aa:Gly,seq:gcc)
     gene            complement(17920..17995)
                     /locus_tag="OQQ48_001777"
     tRNA            complement(17920..17995)
                     /locus_tag="OQQ48_001777"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17960..17962),aa:Gly,seq:gcc)
     gene            complement(18206..18751)
                     /gene="orn"
                     /locus_tag="OQQ48_001778"
     CDS             complement(18206..18751)
                     /gene="orn"
                     /locus_tag="OQQ48_001778"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HCS1952580.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDSFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            18846..19898
                     /gene="rsgA"
                     /locus_tag="OQQ48_001779"
     CDS             18846..19898
                     /gene="rsgA"
                     /locus_tag="OQQ48_001779"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HCS1952581.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNELLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            19995..20963
                     /gene="psd"
                     /locus_tag="OQQ48_001780"
     CDS             19995..20963
                     /gene="psd"
                     /locus_tag="OQQ48_001780"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HCS1952582.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            20985..24308
                     /gene="mscM"
                     /locus_tag="OQQ48_001781"
     CDS             20985..24308
                     /gene="mscM"
                     /locus_tag="OQQ48_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HCS1952583.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERTREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(24459..>24756)
                     /locus_tag="OQQ48_001782"
     CDS             complement(24459..>24756)
                     /locus_tag="OQQ48_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002459969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCS1952584.1"
                     /translation="LPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVT
                     FANVFTIIPPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
BASE COUNT         5698 a         6913 c         6290 g         5855 t
ORIGIN      
        1 tttcttaata agaaggtatt aaattcatct ggattgctga tactacttac cggacaaata
       61 aaggtttcga taatcatttg gcgggaagta aaaagaatat tgatttgcaa atcgccataa
      121 tcattcattt taataattaa tgcgttctcg ctatttgtta catcaatatt ttgttcaggt
      181 acagtttgca gcgccgtcgc cagcgccaac agattccatg tcatatatta ttccatatag
      241 atttagttta aatattaata aaatgaatat ttgcagtacg taattatctt accagctata
      301 gacaaaaaaa aaccatccaa atctggatgg cttttcataa ttcagaggaa ctagctgcgc
      361 tgacgcaccg cttcaaataa gcaaattccg gttgcaaccg aaacgttcag agaagaaaca
      421 cttcctgcca ttgggatgct gatcaactca tcgcaatgtt cacgggtcag gcgacgcata
      481 ccttcacctt ccgcgcccat caccagcgcc aggcgtccgg tcattttgct ctgatagagt
      541 gtatgatccg cctcacctgc cgtaccgacg atccagatat tctcttcctg caacatacgc
      601 atggtacgca ccaggttagt cacccgaatc agcggaacgc tttctgccgc gccacaggcc
      661 acttttttcg ccgtggcgtt gagctgggcg gagcgatctt tcggcacaat caccgcatga
      721 acaccagcgg catctgcgct acgcaggcac gcgccgaggt tatgcggatc ggttacaccg
      781 tcaaggatca gcaggaacgg ctgatcgagc gaagcgatca gatccggcaa atcattttcc
      841 tggtactgac gtcctggctt cacgcgggcg ataatgccct gatgcacggc accgtcgctt
      901 ttctcgtcga gatattggcg gtttgccagc tggataacca cgccctgggc ttcgagggcg
      961 tgaatcagcg gtaacagacg tttatcttca cggcctttta aaataaagac ttcctgaaaa
     1021 cgttcagggg cgcgctccag cagggcctgc actgcgtgga tgccgtaaat catttcgctc
     1081 attaatgtac tcgttgttta cgttttgcct gatgcactac gtttatccgg cctacatgat
     1141 ctctgcaata tattgaattt gcgtgattat gtaggccgga taaggcgttc acgccgcatc
     1201 cggcatgaac aaagcgcatt ttgtcagcaa tctaaccctc ttctgtttga agagggtaat
     1261 gatcactctg ccactttttt cttcgccgca cgcttcgctt tggtcgctgc agctattttc
     1321 tgcgttttcg ccgatggctt tttcgctttt ctcgcgtctt tcttcgctgc tttcggcttc
     1381 gtttttttct caccgcggaa ggcgctgtct ggctcaaagt ttaccttttt accgaccaga
     1441 cgacgcttgc cgcctttttt acctgcatcg ccttttttcg ctttctcgcg cgccgtttta
     1501 ccgacgttgc gcggtgcgcg ttcgctggag atcaggctaa agtcgatttt gcgctcgtcc
     1561 atattaaccg cttcgacgcg aacttccacg cgatcgccca gacgataagt ctggccgctg
     1621 gattcaccca tcagccgttg ccctacctgg tcaaagcgat agtagtcatt gtccagcgaa
     1681 gagacatgga ccagaccatc aatgaataag tcgtccagac ggacaaagaa tccaaagcca
     1741 gtgacgctgg aaattacgcc tttaaagacg ttacctacct ggtcgagcat gaagtcacac
     1801 ttcagccagt cagccacatc gcgcgttgct tcgtcggcac gacgttccgc catcgaacag
     1861 tgctgaccca gttgcagcat ttcttccatc gaataatggt agccgccggt ttcagtggtg
     1921 ttgccctgat gcccctgctc tttcgccagc agatatttaa tggcgcggtg cagcgtcagg
     1981 tctggataac gacgaatcgg cgaagtaaag tgcgcatagg actgcaatgc caggccaaag
     2041 tgaccacggt tttctggatc gtaaatcgcc tgtttcatcg agcgcagcag catggtttgc
     2101 agcatttctg catcaggacg atcggcaacc gactccagca gctccgcgta gtcacgcggt
     2161 tccggcttgt taccgcccgg cagttccagt cccagctccg ccagtactga acggaaagag
     2221 gtaatcgctt cggtgctcgg cttgtcgtga atacggaaca atgctggttc tttggctttc
     2281 tcaacgaaac gcgccgccga gatattcgcc agaatcatgc actcttcaat taacttgtgt
     2341 gcgtcgttac gctgagtctg ttcgatacgc tcaatacggc attcagcgtt gaaaataaac
     2401 ttcgcttctt cgctctcaaa tgagatcccg ccacgttctt cacgggcttt atccagcact
     2461 ttatagaggt tatgcaactc ttcgagatgc ttaaccagcg gagcgtactg ctcgcgcaga
     2521 tcctgatcgc cctgcagaat atgccagact ttggtgtagg tcagacgcgc gtgagagctc
     2581 atcaccgctt cgtagaattt gtagcccgtc aggcggcctt tcgacgaaac cgtcatctcg
     2641 cacaccatac acaggcggtc tacctgcgga ttgagcgagc acagaccgtt agagagcact
     2701 tccggcagca tagggataac ctgcgaaggg aagtacaccg acgtaccacg attacgcgct
     2761 tctctatcca gcggcgttgg cggacgcaca tagtagctga cgtcggcaat cgcgacccat
     2821 aaacgccagc cgccgccgcg ttttttctcg cagtaaaccg catcgtcaaa gtcacgggcg
     2881 tcttcgccat caatggtgac cagcggtaaa tcgcgcaaat cgacacggcc cgcttttgct
     2941 tcttccggca cttcttcttt cagcccggca acctgttgct caacagcctg cggccagatg
     3001 tacggaattt catgggtacg caaagcgata tcaaccgcca tgccggtgcc catattgtcg
     3061 cccagcactt cgacgatttt acccaccgct ttggtacggc gagtcgggcg ttgggtcagt
     3121 tcaacaacca ccacaaagcc catccgcgcg cccatgatct gatcgggcgg gattaagata
     3181 tcgaagctca gacggctatc gtcaggaacc acaaagccga cgcccgcttc ggtaaagtag
     3241 cgaccaacaa tctggctggt ttttggcacc agtacgcgga caatacgcgc ttcacgacga
     3301 cctttacggt cagcacccag cggctgagcc agcacctgat cgccatgaat gcaggttttc
     3361 atctgctcgc tggagagata caaatcatct ttacgccctt caacccgcag aaagccgtag
     3421 ccatcacggt ggccaataac ggtacctttc accaggtcga ggcgttccgg cagcgcatag
     3481 cactgacgac gagtgaagac cagttgacca tcgcgctcca tcgcgcgcag gcggcgacgc
     3541 aggccttcaa gctgctcttc gccttcaatg tgcagttcta ccgccagctc atcacggctg
     3601 gccggttttt cacgtttggt taaatgttcg aggataaatt cccgactagg gatgggattc
     3661 gcgtattttt cagcttcgcg ttcctggaaa ggatcttgtg acatctcggt tcctccgttg
     3721 tcatctctga tgaagatttt cgtcactcca ccagcaataa tttataaagc ggttgattct
     3781 cttcaaccaa atcggcaagc gtgtagttat ccagttccgt aagaaaactt tgcacggcct
     3841 tagaaagcgc ctgtttcaac cgacaggcag gtgtaatgtg gcaaaactca ctgctgcaat
     3901 tcaccagcga taagggctcc agctcgcgca ccacatcacc aatacgtatc gcactcgccg
     3961 gtttacccag gcgaatgccg ccattttttc cacgaacagc agtcacgtag ccggcacgac
     4021 taagttgatt gattattttg accatatgat tacgggagac gccgtagacg tcagtcactt
     4081 cagaaatact ggtcatccgc ccttctggca atgacgccat gtagatcagc gcacgtaatc
     4141 cgtaatcagt gaaactcgtt aactgcacat caacctcaaa agggaaatcg ggaaaaaata
     4201 catttatatt gatgataaac cagccacaag ctgtgtcgct aatttatttc agttcgggga
     4261 aggaaaaaag cgaggattta acactgtgtc ggatagcgta atggcttaac cgacacagca
     4321 atgtatcaca ctgttagccc ggcaggcaaa atatctgcca ggcgtaccag aattacgcgt
     4381 cgaacgggtc gcgcagaatc atagtttcag tacgatccgg accggtagag atgatatcga
     4441 tcggcacacc ggtcagctct tcaatacgct tgatgtagtt cagcgccgcc tgcggcagcc
     4501 cgctacgatc tttcacgccg aaagtggatt cagaccagcc cggcatggtt tcgtaaatcg
     4561 gctctacacc tttccagtcg tcagctgcca gcggagtggt agtcacttcg cgaccatccg
     4621 gcatacggta agccacgcag agtttaacct ctttcaggcc atccagaacg tccagtttag
     4681 tcaggcagaa gccagacagg gagttcagct gtaccgcacg acgaacggca acggtgtcca
     4741 gccagccggt acgacgacga cgacccgtcg ttgcgccgaa ttcgttaccc tgcttgcaga
     4801 ggaactcgcc agtttcatca aacagttcgg tcgggaaagg acctgcaccc acacgagtgg
     4861 agtaagcttt gaggataccc agaacgtaat caacataacg cgggcccagg ccggaaccgg
     4921 tcgccacgcc accagcagtg gtgttggaag aagttacgta cggataagta ccgtggtcga
     4981 tatccagcag cgtaccctgc gcaccttcaa acatgacgaa atcgccacgc tgacgcgcct
     5041 ggtcgagcag gtcagaaacg tcaaccacca tagaagtcag gatgtcggca acagccatcg
     5101 tatcatccag aactttctgg taatcaaccg cttcagcttt gtagtagtta accaactgga
     5161 agttgtgata ttccatcact tctttcagtt tttcagcgaa ggtttctttg tcgaaaaggt
     5221 cgccaacacg cagaccgcga cgtgccactt tatcttcata agcaggcccg ataccacgac
     5281 cggtggtgcc gatcgctttc gcgccacgcg ctttctcacg cgcgttatcc agcgcaacgt
     5341 gataatcaag gatcagcgga catgcttcag acagcagcag acgctcacga acggggatgc
     5401 cacggtcttc cagttctttc atctctttca tcagcgcggc cggagacagc acaacaccgt
     5461 taccgatgat gctggttaca ttctcgcgga gaatacctga tggaataaga tggagaacgg
     5521 ttttttcacc gttgattacg agagtatggc ctgcgttgtg accgccctgg tagcgtacaa
     5581 catatttagc tcgttcagtc agaagatcga cgatcttacc tttaccttcg tcaccccatt
     5641 gggtgcccag tacgacgacg ttgttaccca tttttcaaaa tcaccgtttg cttaaaaatg
     5701 gattctacca tcgctttttc agagttacag cactttttgc actcaaaaat gacctcgatc
     5761 agtctgtttt ttgttcagcc aatcgttttc ctcaacatgt agtagaccac aacgcccgca
     5821 accacaagtc caccgccaaa acgacgtaaa atattatcgg gcaaattggt catcgcagag
     5881 atcatcttct tccatgcctt cgggtaaagc atcggcccta aaccttccag taccaaaacc
     5941 agggcaagcg ccagccagat tgtcgaattc attcattatc cttatagaaa aagaaaacca
     6001 ccgacatccc tgaggatgcg gtggctttat tgacctgtac cgcagtcgtt atattaacgc
     6061 gttgcggaag tcggcgtctt catgtagcgg aagaaatcgc tatccgggct catgaccatc
     6121 acgtcctgat tgccagagaa gctgttctca taagcacgca ggctacggat gaatgcgtag
     6181 aagtccggat ctttactgaa tgcatcagca aacagtttgg ctgcttcggc atcgccttca
     6241 ccacgcatga tgcggccctg acgctcagct tctgccagcg ttctggtcac ttcatagtcg
     6301 gcagtcgcgc gcagtttttc cgcttcttcc tgaccttgtg aacggtgacg acgcgctacc
     6361 gcttcacgct cggcgcgcat acggttgtag atcgcttcag acacttcggt cggcaggttg
     6421 atctgcttga tacgcacatc gacaacttca atacccagcg ccgccatact gttcgggttg
     6481 atgaccggaa ctttgccctt cgtctcagcc gttacgcgct ctgccgcttc ggcaatggcg
     6541 ttatctgctg ccggggtagt aacttcatct tctgtacccg cagaaccgga gttcagcgcg
     6601 tcacgtactt cgagggtcag acgaccacgg gaatcagtga cgatatcttt cacgtccagg
     6661 cgaccaattt cagaacgcag acggtcagag aacttacgtt tcaacagcac ttccgcttgc
     6721 gaaatgtcgc caccacccgt tgccaggtag taacggctga aatcgctgat gcgccatttg
     6781 atgtaagagt cgacgatcag gtctttcttc tctttggtca caaagcggtc ggcctggttg
     6841 tccatggtct gaatacgtgc gtcgagcatt ttcaccgttt caatgaacgg tatcttgaaa
     6901 tgcagacccg gctcataaac cagaggtttg ttgtcatcgt cacgcagtac cttaccaaaa
     6961 cgcagcgtaa taccgcgctc accttctttg acgacaaaga cagacatgta aagcactacc
     7021 agcacgatga tgataatcgc gataactgac ttacgcatcg ttattccccc tgacgctggt
     7081 agtcgttacg ctgcgcgttg gcgcggcgtt ggtccataat atcgccctga ctggtggacg
     7141 acgtgttgct tgctccactg gttgtggagg aagaggctgg cggcaaacgc agcagattgc
     7201 tggcaccgtt atcgctcttc gccgcagggg cgttaccacc tttcagcatc tggtctaacg
     7261 gcagaaccat caggttgcca cctttatcgt taaccagcac tttgcgggtg ttacccaaca
     7321 ctttttccat cgtctcgata tacagacgct cgcgagtaat ttccggcgcg gctttatatt
     7381 ccggcagaag tttagcaaag cgcgccactt caccctgagc ttccaggatg gtctgggcct
     7441 tgtacgcacg cgcctcttcg aggatacgtt gcgcctgacc gttcgcacgc ggctgaactt
     7501 cgttggtata cgcttctgct tcacgaatgt attgctgttc gttttcacgc gcggcaatcg
     7561 catcgtcaaa cgccgctttt acttcttccg gcggacgagc agcctggaag ttgacgtcca
     7621 gcagagtgat gcccatgtca tacggacgaa tcgtctcttc cagttcgcgc tgggtatcgc
     7681 tacgaatcac ggtacgacct tccgtcagaa tgcggtccat ggtgtattta ccgataaccc
     7741 cgcgcagggc gctgtcggta gcctgacgca ggctgtcatc cgggctggtc acgctataca
     7801 gatatttttc cggataggta acgcggtact gcacgttcat ctcaacgcgc actacgttct
     7861 cgtccgacgt cagcatcaca ccggaagcgg ccagttcacg cacggcttcc acgtttaccg
     7921 gtttgacttc gtcgataaac gtcggtttcc agttcagacc cggctcaacc agatggctga
     7981 atttaccaaa gcgtgttacc acgccgcgtt cggcttcttt aatggtatag aaaccactgg
     8041 ccgcccagat aatgacaatc gctgccgctg cgatggtaac gacacgaccg ccaagctgcg
     8101 ggcgcgggcc ttgcgatgaa ctgccaccgc cagatccggt gcctttaccg ccgcccagac
     8161 caccgagctt tttgctcagt ttgcggaaga tatcatctaa atcaggtggc ccttgatcgc
     8221 gaccgccttt gtttccattt ccctcagagt tgccgccagg tttgctgctt ccccacgggt
     8281 cgcggtcttg tccgttatta ccgggctgat tccacgccat gtttgtgctc catatttgtt
     8341 atgcggtgat ccctgttgtc tttcgacgcc agaggatatg actccacgct tcagacgcta
     8401 cgccgttaga tcaggtaatc gatcaacgcc ggttcttgtt tacagaggcg acgccagtca
     8461 acgatcggca tacgaacttg cagacttacg ctgccgtcct cctccatcca ctctttttct
     8521 attgcctgaa gctgataaaa acgacttctc agacgccctt cctgcggtgg cagacgcaat
     8581 gtatgctgcg ccacctcgcc ggaaagccgc tccgtcaaag cctgaaaaag ctgtggtatc
     8641 cccgctccgg tctgtgcgga aagccagaca cggatcggtt tgttctcttc gtcccgatca
     8701 atacgcggtt cgaaatcttc cagcatatcg atcttgttca tcaccagcag ggttgggatc
     8761 tcgtgagcgt cgatctcttc aagaaccgta ttcaccgctt cgatgttttc ttgtacacgc
     8821 acatccgccg catcaatgac gtgcagcagt aatgtggctt gccgcgtctc ttgtaacgtg
     8881 gctttaaatg ccgccaccag atcgtgcggc agatggcgaa taaaccctac ggtatctgca
     8941 agtacggttt caccgacatc cgcgacgtca atacgccgca acgtcgggtc gagggtggca
     9001 aacaactggt ctgccgcgta gacccgcgct tcggtgatgc gattgaaaag ggtagattta
     9061 ccggcgttgg tatatcccac cagcgaaaca gtaggaacgt cggctttgat acgcgattgc
     9121 cgcccctgct cacgctgctt ttcaactctt tccaggcgcg actgtatctg cacgatgcga
     9181 ttacgcaaca aacgacggtc ggtttcgagc tgggtttcac ccggaccacg caaacctatc
     9241 ccgcctttct gtctttcaag gtgggtccag ccacgcacca ggcgcgtagc cagatggcgc
     9301 agctgcgcca gctcaacctg caacttaccc tcatgggtac gcgcacgttg ggcgaaaata
     9361 tctaaaataa ggccggtgcg gtcgatgaca cgacactcgc acaaacgctc caggttacgc
     9421 tcttgcgccg ggctcagggc atggtcaaaa agaacgaccg aagcacccgt cgctttgaca
     9481 gcttccgcaa tttcaactgc tttaccttca cctacaaaat actttgggtg cggcgcttta
     9541 cggctaccgg taatcacctg caatgcttcg acaccggcgg aagaaaccag agattcaaac
     9601 tcctggaggt cttccatatc tttgtctttc gtaaaataga tgtgtaccag taccgcctgc
     9661 tcaccagcat cataacggtc aaacaagcgt ataaccctct aaatagatca gcggggaacg
     9721 caggatcgct ggctccccgt gtaaaaaaaa cagcccgaaa ccttattcgg tttcttcgct
     9781 gtcctgttgc gcggaagtat tctgcgcgct gctaccatga tggtagttac tgctggtacc
     9841 gccaccggcg ttgttactgt gatgagaaac cggacgagac gggacaacag tagaaatcgc
     9901 gtgcttgtaa accatctggc tgaccgtgtt tttcaacagg atcacgaact gatcaaaaga
     9961 ctcgatttgc ccttgcagct taataccatt caccaaataa atagaaactg gaacacgttc
    10021 ccgacgcagt gcgttcagga acggatcttg taaagattgc cccttagcca ttctctcttt
    10081 tccttatatg cttatttgta ctttgaacct ttcgattctg aaaaaattgc gcacgatacg
    10141 tctcaattgt acacattcag cctgcgatag caccaacaac ctgtaatact tcgtcacgcg
    10201 cctgttctgg tttttcactg tcaagccagt gaaccccttc ccaaccacgc agccaggtta
    10261 tctgccgctt cgccaactgt ctcgtggcgc aaacacctcg ataaaccatt tcatcgtatg
    10321 agatttcgcc ttcaaggtaa gaccacatct ggcgataacc cacgcaacga atggaaggca
    10381 agtccgtatg caaatctcct cgggcaaaaa gcgcccggac ttctgcttca aaacctgaag
    10441 ccaacatctg atgaaaacgc tgctcaatgc gttgatggag cagttcacgg ctcgccgggg
    10501 cgatggcgaa ctgatgcacc tgatacggta gagcgtctcc tgacgtttgc gtcagttccg
    10561 ttaaagtttt acccgaaatg aaaaaaactt ccagtgcccg ggaaagcctt tgtggatcat
    10621 ttggatgaat ccttgctgcc gcaaccggat ctacctcctg aagttgacga tgcaatgact
    10681 cccaaccttg ctctgccgcc tgttgctcaa ttctggcccg cacttccggg tctgccgacg
    10741 gtagcggcga caacccttcc agcaatgcct tgaaatacaa catcgtaccg cccactaaca
    10801 gtgggatccg ccccgccgcg gtgatatcgg ccatttccgc cagcgcatcg cggcgaaaat
    10861 cagcagccga gtaagcctgc gacggatcgc gaatatccag caatcggtgc ggcgcggcga
    10921 gtaactcttc agcgttcggc ttcgccgtcc cgatatccat ccctttgtaa ataagggcag
    10981 aatcaacgct tatcaactct actggtaaaa ttttacgcag ctcaatggct aacgccgttt
    11041 taccggaggc cgtcggcccc atcaaaaaaa tcgccttagg caggctcgcc ttactgatat
    11101 cactcatctt tcagggcttt tatcgccgga tgtaaatcaa cagattgtaa cagaccaccc
    11161 ggcggcgttt tcacaagttg cggacataac cgttccacgt ccgccagcag ggttatggcc
    11221 tgtgccattg accactgcgc atgttcgctc atcacatttc gtgcaatcca ctgcgcaata
    11281 ttgccaggtt cgaatacgga ctgcttcgcc aggtagccta tcagttcagg aatcaagatt
    11341 tgtaaatttt gttggcgtaa gggtaaaggc acggccctga tggtcacatg ctgtgcatct
    11401 gactggaaat caatacccaa ttccgccagg gcagactgcg ctttttctaa tgccgatttt
    11461 tcttcgccag aaacttttag ccgcaacgga ataagcagcg gctgagcgca aactggcgct
    11521 tcacccgggg tcagttgtac ctgacgcagc caacgttctg ccactggcaa ggctaaaagt
    11581 gaaatgttgc cgtcgcgctc cagcaacgca cagtcggaat ggacgatagt cagtacccga
    11641 ccaaaactct gactgttcgc cgcaagtgca ggttcctgcg gttccggcgc ttttaatttt
    11701 tgcatcggcg cgggcgtttg caaaagctgg cgatacactt caccttgctg tttctggtag
    11761 cctggctgcg cattcggcca aggggcagcc ggacgactgc ctgatgctgg cgcaggagtg
    11821 tagcgcggag ctaccggctc acgagctgcc ggttctgcaa agtgattgcg ccccgccgcc
    11881 acgcggtttt ccggaatgga acgcggtgca ggttggggtt catcgtccag cggtagcggc
    11941 gtttccagtt gctgttgtag cacgctcagc acgccctgat agataaaatc atgcaccaga
    12001 cgcgactgat ggaaacgcac ttcgtgtttg gcggggtgca cgttgacgtc cacctgatgc
    12061 ggatcgatct ccagatacag cacaaatgcc ggttgctgat cggcccccag tttgtcttcg
    12121 caggcctggc ggatcgcgtg attgatcagg cgatcgcgca tcatgcgacc gttcacgtag
    12181 caatactgaa tttctgccag tgcgggcgtg gtgtgatttg gatcggccac ccagccgcgt
    12241 agcgtgagat cgccgtgttg ccattcaatc gccagcgctt gttcaagaaa agcggtgccg
    12301 caaatcgcgc ctaagcgccg ttctttttgc ccgccttccg gcactgcgcg gtactgacgc
    12361 acaattttac cgttatgcga caggttgatc gtgacgtcga aacgcgccag cgcaatgcgg
    12421 cggatgatct catcaatgtg gttaaattcg gttttctcgg tgcgcaggaa tttgcgccgc
    12481 gccggggtgt tgtagaacag atccagcacc tccagcgtcg tccccacagg atgcgccgcc
    12541 ggttttaccg tcacgttcat atcgcgccct tcggcatagg cctgccaggc ttcctgctgt
    12601 tctgcggtgc gtgaagtgag cgtcaggcgg gaaaccgaac tgatactcgc cagcgcctca
    12661 ccgcgaaagc ccaggctgat aatggcttcg agatcgtcca gagaggcgat tttactggtg
    12721 gcatgacgag ccagcgccag cgccagctca tcttttttga taccgcagcc gttatcacga
    12781 atgcggataa gtttcgcccc accgcgttcg atatcaatat cgatacgcgt cgcacctgca
    12841 tcgaggctgt tttccaccag ttctttgact accgacgcag gtcgctcgac cacctcacct
    12901 gcggcaatct ggttcgccag ttgtggcggt aagacctgaa ttggcatcaa tcgtccttag
    12961 tttggcagcg tgcgatctgg cgtcgtcacc gtactggccg tttgtgccgt tgcaccctgc
    13021 ggcgcagatt gcatcggatg cgcaaggaaa taattgcgca ggcctttgta aatggcttct
    13081 gccagctgtt gttggtaatc gtcgctcgcc agcaaacgtt cttcgctgtt gttgctgata
    13141 aaaccggttt cgaccagtac tgacgggata tccggcgaac gcagaacgcc gagactcgcg
    13201 tgttctggtc ggcgtttatg tatttcgcca atgcgttgca actgactgat catactggtc
    13261 gctacatcat accctacccg ctgggaatga ccgaactgta aatccagcac cgcctggctt
    13321 aaatagggat cagactgact gttcgccagc acatcgcccg ccccacccag cagctccgaa
    13381 tgtttctcgt gctgctccag ccagctggcc atttcgctgt tggcgcgacg gttagagagc
    13441 acccatacgg aagcgccagt cgcactgcgg ttcggtgcgg catcagcgtg aatcgacacg
    13501 aggaaattag cgttttgctt acgtgccaca tcgctgcgcc ccatcaccga aataaagtaa
    13561 tccccgtcac gggttaaaac gcctttaaac atcggatcgt cattgagcaa agtacgcaat
    13621 ttacgcgcga tggcgatggt gacatttttc tcccgcgtac caccggggcc gatagcgcca
    13681 gggtcctgac cgccgtgtcc ggcatcaata gcgatgataa ttttatcgcc agtgttagcc
    13741 gtcgcgcgcg ctgccggacg cgttaccgta ttactgctga taacacccgt agtgcggtta
    13801 ctttccgttt taaacggatt gcgcgccggt tcgctgacgc gcggtgcgac aaccgcaggc
    13861 gtttcaacgc gtttcgcaac cacgggcggc ggaggaggcg gtggcggcac atcggcgtta
    13921 atcgtaaaga cgacagtgta attgctgcca ttctgccgct tcaccgcttc ggttttacca
    13981 ttttcggtaa gatcgaccac cagccgtagc gtttgtgcat ctttaggcgt tccagagcga
    14041 atcgccttca ccagattatt gccgctgaac aacaacggca gtccctgaat cacgcccgtt
    14101 tgtttgatat cgagcgccac ggtgcgtttg ctttgatggc taaacgcata atcaggatcg
    14161 ccaataaaac tcaacgttat ccgcgcctgt tgattaccgt tagaaacctg aatatcagag
    14221 agcgtcgcgg cacccaccgg cgtgcacagc agcagcagcg tcgctaccaa ccaatttctg
    14281 atgcgataca tcatcccgcc acctttcaaa ggttaaccgg ctaaacgcgc cagcaacaat
    14341 tcacccgcag aggaaaccgc actcacgcgc gcctcacggc cttgtgcctg ataatcaatg
    14401 tgtatttcga catccgggtc aggaagaaca cctgtacctt gttgtggcca ctccaccagg
    14461 cagatggcat cgttggcaaa ataatcgcgg atccccataa actccagctc ctcgggatcg
    14521 gcaaggcggt acaaatcaaa gtgataaacc attaaattgt cgagcgtata gggttcgacc
    14581 agcgtataag tggggctttt gacattaccc tgatgaccca gagcttgtaa aaagccccgg
    14641 ctaaaggtgg tcttacctgc gcctaaatcg ccatacagat agattacggt tgcgccatcg
    14701 caggctttcg ctacccgctc gcccaggtct aatgttgcct gctcatcagg gagcggaatt
    14761 actcgattca tcatggtttt tatcagtcac ttccgggtta acaatacgct gtagcgtgga
    14821 aaagagatcg gttgccagca tcccgcgcgt tccaaaacgc gccgccagta cgtcagctgc
    14881 cgcaccgtgc gcgacacagc ccgcacaggc ggcatcatac ggcgacagtt tttgcccaag
    14941 caatgcgcca ataataccag agagcacatc gcccatgccg ccgctcgcca tgcctgcatt
    15001 tccggcatca ataatgccta aagcgtcagg atgggcggcg accacggttc cggcaccttt
    15061 cagcaccgct acgccgccat aacgttgtac cagacgtttg gcgcaatgta agcggtcact
    15121 ttcaatttca gcgacggaac agcctaacaa ccgtgcggcc tcgccaggat gcggcgtgat
    15181 cacgcgattg tgacgcttat cgggattgat tgccagcagg ttcaatgcat cggcatccca
    15241 caacatcggt ttgcgaaaat tctcaacttt ttgcagtgct tttttccccc actcttgctg
    15301 gcccagaccg ggaccaatca ccaccacatc ggcccattcc aggctttcgg taagagagtc
    15361 catcgtcagt tcatgcacca tcaattccgg tcgtgcagtc agcagcggcg caatgttttc
    15421 actgcgggtc agtactcgga ctaaaccagc accagcacgc agcgccgctt cccccgtcat
    15481 acgaatagcc cccgccgtgc cgtgatcgcc accgataatt accagccgcc cgtgatcgcc
    15541 tttatgcgaa gtcgggcgac gcggttttag ccagtgagaa agttgttctg ctgaaaaccg
    15601 ctgaattttc gtctcctgac ctgccagcca actatccagc cccagtgagt caaaatgcag
    15661 ttgtccggta acatcccgcg cttttccagt gagcaagcct ggtttcagcg caataaaagt
    15721 gatggtgtga tcggcgttga tcaccgcgcc tggcgtagcg ccagtttcag ccagcaggcc
    15781 ggaagggata tcaaccgcca caatcggcgc aggatgggaa ttagcgtggt cgattaactg
    15841 gctaatggat tcgcggggcg cttgccgcaa accggtaccg agcagcgcat caacaatcag
    15901 atctaccgat tcgggccaga caatattcga agcatggatc tcgccacccg cgtttaacca
    15961 tgcttcgcgt gccagcgcgg cctcttccgg caacggtttg tcgctctcct gggcaaacaa
    16021 cgtgacctca atgccgaccg ctttggccag tcgcgcgacc acgtagccat cgccgccgtt
    16081 attaccatga ccgcacagca ccagccagtg gcgggcgtca ggatacgccg aacgacacac
    16141 ctggaatgcg gcctcgccag cgcgaagcat cagctcatag agtgtgagcc ccagcacatc
    16201 tgccgcctcg cgttctccgc ggcggatatc gtcggcgtac cagacggtgt gtggtatact
    16261 tacggggttt ttcttcattg tatggtccgt catgtcagag cccctcgatc tcaatcagtt
    16321 agcgcaaaaa attaaacagt gggggctgga actgggcttt cagcaggtag gtattaccga
    16381 taccgatctc agcgagtccg agcccaaact gcaagcatgg ctggacaaac aataccacgg
    16441 cgaaatggac tggatggcac gtcacggtat gctgcgcgct cgccctcatg agttattgcc
    16501 cggtacgctg cgcgtgatca gcgtgcggat gaattacctt cctgctaacg ccgcatttgc
    16561 cagcacgctg aaaaacccca aactcggcta tgttagccgt tatgcgctgg gccgtgacta
    16621 tcacaaactt ctgcgcaacc gactcaaaaa gctgggcgag atgattcagc aacattgtgt
    16681 ttcgctgaat tttagaccgt ttgtcgattc tgcgcctatt ctcgagcgcc cgttagctga
    16741 aaaagctggg ctcggctgga caggtaagca ctcacttatc ctcaatcgcg aggccggttc
    16801 gttcttcttt ttaggcgaat tgctggtcga tattccgctg cccgtggatc aaccagtcga
    16861 ggaaggatgc ggtaaatgcg tggcctgtat gacgatttgc ccgaccggtg ccatcgtcga
    16921 gccatatacc gtcgatgctc gccgctgtat ctcttatctc accatcgaac ttgaaggggc
    16981 gatcccggaa gagttgcgac cgttaatggg aaaccgtatt tacggttgcg atgactgcca
    17041 gcctatctgc ccgtggaatc gctattcaca actcaccaca gaagaggatt tcagcccgcg
    17101 taagccgcta cacgcaccgg aactcattga gttattcgcc tggagcgaag agaagttttt
    17161 aaaagtcacg gaaggatcgg cgattcgtcg aattggtcac ctgcgttggc tgcgtaatat
    17221 cgccgtagca ttaggcaatg ccccttggga tgaaacgatt ttgacagcgc tggaaagtcg
    17281 taaaggtgag cacccacttc tcgatgagca catagcgtgg gcgattgcgc agcaaataga
    17341 gagacgaaat gcgtgcatag tcgaagtgca attgccgaaa aaacagcgtc tggttcgggt
    17401 gattgaaaaa gggttaccgc gtgacgcctg attcatccac agcttgtgaa taaaaataaa
    17461 aacgcattgc aattcaagac gaataaaatc gtcaagtgat cgactcgaca aaacggaatc
    17521 ttaattttta acttatctat caataggtta aaaaaatact attcaccgtg cgaagtttct
    17581 ttgaaattaa gattacagcc tgaggctgtg gataactctg tttacaagag tttgtcagag
    17641 acaacgaaaa ccagcaataa cgcatcgctg tggatatttt attgagagaa gaatttggag
    17701 cgggaaacga gactcgaact cgcgaccccg accttggcaa ggtcgtgctc taccaactga
    17761 gctattcccg cttgggtggt ctgtgcctta cagcactttt caaattttgg agcgggaaac
    17821 gagactcgaa ctcgcgaccc cgaccttggc aaggtcgtgc tctaccaact gagctattcc
    17881 cgcttgggtg gtctgtgctt tgcagcactt ttcaaatttt ggagcgggaa acgagactcg
    17941 aactcgcgac cccgaccttg gcaaggtcgt gctctaccaa ctgagctatt cccgcgtact
    18001 acttaatttt gctttcgtaa ttttcgcatt tctgcgtcgt tacgggaggc gtattatacg
    18061 agaaatcttt ccctctgcaa ccccccaaaa aagttttttt tgcgttttta tctcaagtgc
    18121 tgaaattatc agcaagacgt tcaatttagc gtcaaaatca cgccgccgca agtggattcc
    18181 ggcgcttacg tgaccaggaa aaattttaca gcttgataaa atgctcgcgg tagtaagcca
    18241 gctccgccac cgattcacgg atatcatcca tcgcctgatg cgtcccctgc ttggtaaaac
    18301 tatccagaat ttccggcttc cagcggcgcg ccagctcttt cagggtgctg acatcgagat
    18361 aacggtagtg gaagtaggct tccagctccg gcatgtattt aaacaggaaa cgacggtcct
    18421 gtccgatgct gttaccgcaa atcggcgatt ttcccgcagg cacccactgt tttaagaatt
    18481 cgagcgttgc cagttcagct tcccgatcgc ccatcgtgct cgctttcacg cgctctacca
    18541 gcccgctggc ggtatgggtg cgcacgttcc agtcatccat cagcgccagc tgttcatcgg
    18601 actgatgtac tgcaatggtc ggcccttctg ccagaatatt caggttggca tcggtcacca
    18661 gcgtggcaat ctcaataatg cgatcgcgct cgggatccag accagtcatc tcaagatcga
    18721 tccaaatcag gttgttttca ttggcactca tgctattttc cacccattca cagtgactat
    18781 attgatccac aattgcgtgt atcatagatg ttttgcccat caggggcgac caggagtctg
    18841 tacgattgag taaaaataaa ctctccaaag gccagcagcg ccgcgtgaac gccaatcacc
    18901 agcgtcgtct taaaacgtct aaggagaagc ccgactacga cgacaatctg tttggcgagc
    18961 ctgatgaagg tatcgtcatc agccgctttg gtatgcacgc tgatgtggaa tccgccgatg
    19021 gcgacgttca ccgctgcaat attcgccgta ccatccgttc gctggtgacc ggcgaccgtg
    19081 tagtctggcg tccgggtaaa ccggcggcgg aaggcgtaaa tgtcaaaggg atcgtggaag
    19141 cggtgcatga gcgtacctcg gtgttgacgc gtccggattt ctacgacggc gtaaaaccta
    19201 ttgccgccaa catcgaccag attgtcattg tctccgccat tttgccggag ctgtcactca
    19261 atattatcga ccgttacctg gtggcctgcg aaaccttgca gattgagccg attattgtgc
    19321 tcaacaagat agacctgctg gacgacgaag gcatggcgtt cgtcaacgag cagatggata
    19381 tctaccgcaa tatcggttat cgcgtattga tggtttccag ccatactcag gatgggctaa
    19441 aaccgctgga agaggcgttg accgggcgca tcagcatttt tgccgggcag tctggcgtcg
    19501 gcaaatccag cctgctgaat gagttgctgg ggctgcaaaa agagatcctg accaacgatg
    19561 tctctgacaa ctcggggctc ggtcagcaca ccacgaccgc cgctcggctg tatcacttcc
    19621 cgcacggcgg tgatgtgatt gattcccctg gtgtgcgtga gttcggcctc tggcacctgg
    19681 agccggaaca aatcactcag ggctttgtcg aattccatga ctatttaggt ctgtgtaaat
    19741 atcgcgattg caaacacgat accgatccgg gctgcgctat ccgggaagcg gttgaggaag
    19801 ggaaaatcgc ggaaacccgt ttcgaaaact atcaccgtat tctggaaagc atggcgcagg
    19861 taaaaacgcg taaaaacttt tctgatacgg atgactgaca attaagctaa ccgtcgttag
    19921 aatcgtcccc tttttttcag gatccggcat gttggccgga tcaggaacga caaaacaatg
    19981 gcctggaggc taccttgtta aattcattta aactttcgct acagtacatt ctgccgaaac
    20041 tatggcttac tcgcctggcg ggttggggcg caagcaagcg ggcaggatgg ctgacaaaac
    20101 tggttatcga tctgttcgtt aaatactaca aggtcgacat gaaagaggcg caaaagccgg
    20161 acaccgccag ctaccgcacc tttaacgaat tctttgtccg tccgctgcgt gacgaagtac
    20221 gcccaatcga taccgatccg aatgtactgg tcatgcctgc cgatggcgtt atcagccagt
    20281 tgggtaaaat cgaagaagat aaaatcctgc aagccaaagg ccacaactac agcctcgaag
    20341 ccctgctggc aggcaactat ctgatggcgg acctgttccg caacggtacg tttgtgacca
    20401 cttacctctc cccgcgtgac taccatcgcg tacacatgcc gtgcaacggt attctgcgtg
    20461 agatgatcta cgtgccgggc gatctcttct ccgttaacca tctcaccgct cagaacgtgc
    20521 cgaatctgtt tgcccgtaac gaacgcgtga tttgcctttt cgataccgaa tttggcccaa
    20581 tggcgcagat tctggtcgga gcgacgattg ttggcagcat tgagacggtc tgggcgggca
    20641 ccattacgcc gccgcgcgaa ggtatcatca agcgctggac ctggcctgcc ggggaaaacg
    20701 acggttctgt ggcactgctg aaaggccagg aaatgggtcg ctttaaactc ggttccaccg
    20761 ttatcaacct gtttgcaccg ggtaaagtga atctggttga gcaactggaa agcctgtctg
    20821 tcacgaaaat tggccagccg ctggcagtat ctaccgaaac ctttgttacg ccagacgctg
    20881 aacccgcccc acttcctgct gaagagatcg aagcagaaca cgacgccagc ccattggttg
    20941 acgataaaaa agaccaggtc taatccatca aaggaaacgc tgacgtgcgc ctgattatca
    21001 cttttctgat ggcctggtgc ctcagttggg gggcgtacgc cgcgacggcc cccgatagca
    21061 aacaaatcac tcaggaactg gagcaggcaa aagcggcgaa acccgcacag ccggaagtcg
    21121 tagaggcgct ccagtctgcc ttaaatgcgc ttgaggaacg aaaaggttcc cttgagcgca
    21181 tcaaacaata tcagcaagtt atcgataatt atccgaaact ctccgctact ctgcgcgcac
    21241 aattaaacaa catgcgtgac gagccgcgca gcgtgtcgcc gggaatgtct accgacgcgc
    21301 tgaatcagga aattctccag gtcagcagcc agttgctgga taaaagccgt caggcccagc
    21361 aagagcagga gcgcacccgc gagattgccg attcgctgaa tcaactgccg caacagcaaa
    21421 ccgacgcccg ccgtcagtta aatgagatcg agcgccgcct gggaacgctt accggcaata
    21481 ctccgctcaa tcaggcacaa aatttcgcgt tgcagtctga ctctgcacgt cttaaggcgc
    21541 tcgttgatga actggagctg gcgcagctgt ctgccaataa ccgccaggaa ttagcgcgct
    21601 tacgctcaga gctggcggaa aaagagagcc agcaactgga tgcgtatttg caggccttgc
    21661 gtaatcaatt aaacagccaa cgtcagcttg aggcggagcg ggcgctggaa agtaccgaat
    21721 tgctggcaga aaacagcgcc gatttgccga aagatatcgt cgcgcaattc aaaattaacc
    21781 gcgaactatc ggcggctttg aatcaacagg cgcagcggat ggatctcgtt gcctcgcaac
    21841 agcgtcaggc tgccagccag acgttacagg tccggcaggc gttgaatacg ctgcgtgaac
    21901 agtcgcaatg gctgggatcg tccaatctgc tcggcgaagc gctgcgggcg caggtggcac
    21961 ggctgccaga aatgccaaaa ccacaacagc ttgataccga aatggcgcag ttgcgtgtgc
    22021 aacggttacg ttatgaggat ctgcttaata aacagccgct gctacggcaa atccatcagg
    22081 ccgacggtca gccgctgacc gccgagcaaa accgtattct ggaagcacaa ctgcgcactc
    22141 agcgtgagtt gctgaactca ttgttgcagg gtggcgacac gctactattg gaactgacca
    22201 agctgaaagt ctccaacggg caactggagg atgcgctgaa agaggtgaac gaagcaacgc
    22261 accgctatct gttctggacc tctgacgtgc gcccgatgac catcgcctgg ccgctggaaa
    22321 tcgcccagga tctgcgtcgt ctcatttcgc tggacacctt cagtcagttg ggcaaagcca
    22381 gtgtgatgat gctgaccagc aaagagacaa ttttgccgct gtttggcgcg ttgattctgg
    22441 tcggttgcag tatttactcg cgccgctatt tcacccgttt tcttgaacgt tcggcggcga
    22501 aagttggcaa agtgactcag gatcacttct ggctgacgtt gcgcactctt ttctggtcga
    22561 ttctcgtcgc gtcaccgtta ccggtgctgt ggatgacgct gggttacggc ttgcgcgagg
    22621 cgtggcctta tccgctggcg gtcgcgattg gcgatggtgt aacggctacc gtgccgctgc
    22681 tgtgggtagt gatgatttgc gccacctttg cccgcccgaa cggcttgttt atcgctcatt
    22741 ttggctggcc gcgcgaacgt gtttcccgtg ggatgcgcta ctacctgatg agcatcgggc
    22801 ttattgtgcc gctgattatg gcgctgatga tgttcgataa cctcgacgac cgtgaattct
    22861 ccggttcgct gggacggctt tgctttatcc tcatttgcgg tgcgctggcg gtggtcaccc
    22921 tcagcctgaa aaaggccggg atcccactgt atctcaacaa agagggcagc ggcgacaaca
    22981 ttaccaacca tatgctgtgg aacatgatga ttggcgcgcc gttggttgcc attctggcgt
    23041 cggctgtggg ttatctggca acggcacagg cgctgttagc gaggcttgaa acctcggttg
    23101 ccatctggtt cctgctactg gtggtttatc acgttatccg ccgctggatg ctgatccagc
    23161 gccgcaggct ggcgtttgac cgggcgaagc atcgccgggc agagatgtta gcgcaacgtg
    23221 cgcgtggcga agaggaagcg catcatcaca gtagcccgga aggagcaatt gaagtcgatg
    23281 aaagcgaagt cgatctcgat gccatcagtg cgcaatcctt gcggctggtg cgctcaattt
    23341 tgatgttgat cgccctgctt tctgtcattg tgctgtggtc agaaatccat tccgctttcg
    23401 gcttcctcga aaatatttcg ctgtgggatg tcacctccac ggtacaaggc gtagaaagtc
    23461 tggagccaat taccctcggt gcggtgctga ttgccattct ggtgtttatc atcaccacgc
    23521 agctggtgcg caacctgccc gcgctgctgg aactggcgat tttgcagcac ctggatttaa
    23581 cgccgggtac aggctacgcc atcaccacca tcaccaaata tctgctgatg ctgattggcg
    23641 ggctggtcgg cttctcgatg attggtattg aatggtcgaa attgcagtgg ctggtcgccg
    23701 cgctcggtgt tggtctcggt tttggtttgc aggaaatttt cgccaacttt atctctggtc
    23761 tgattatcct gttcgaaaaa ccgattcgca ttggcgatac ggtgacaatt cgcgatctca
    23821 ccggtagcgt gacgaaaatt aacacccgcg ccaccaccat cagcgactgg gaccgtaaag
    23881 agataatcgt gccgaacaag gcgtttatta ccgagcagtt tatcaactgg tcgctctctg
    23941 actcggttac gcgcgtagtg ttgacgattc cggcccctgc tgatgccaac agtgaagaag
    24001 tgacggaaat cctgctcacc gcagcgcgtc gctgctcgct ggtgatcgac aacccggcac
    24061 cggaagtctt cctggtggat ctgcaacaag ggattcagat tttcgagctg cgtatttatg
    24121 ccgctgagat gggtcaccgt atgccgctac gccatgagat ccaccagctg attctggctg
    24181 gcttccatgc ccacggtatc gatatgccat tcccgccctt ccagatgcgt ctggaaagcc
    24241 tcaacggtaa acaaacgggg agaacgctga cgtctgcggg caaaggtcgt caggcgggaa
    24301 gtttgtaatg catgaagctg ccaaatgatg tcaacgtggc tcatttagca gcttaacatc
    24361 atcttcagtt atcaccggcc ctggagagta tcatccatac gcattacttc ctgacaactc
    24421 atcgccggag agacgatgag ttgcaggatt tcatatgatc agtcgagagc taactcaggt
    24481 ttattcgcca tgcgactgca atagttctgg taaatcgcca tcgctaacag cgagaagaag
    24541 acagggccgc caatcatcca caatgtgctg tcccagtctc cggcttcaac cacaggcgga
    24601 ataatggtga agacgttcgc aaatgtcacc accagtacga caaccaccgt cgcaatcatt
    24661 gccgacatac gcgttttgaa aatcacaaat ggtctgtcga gatcctgacg tgctttaaag
    24721 aatgggaaag ccagcgcgag gaacaggtaa ggaagc
//
LOCUS       DAJVUJ010000048        24740 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.048, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000048 DAJVUJ010000000
VERSION     DAJVUJ010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24740
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.048"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(48..338)
                     /locus_tag="OQQ48_001783"
     CDS             complement(48..338)
                     /locus_tag="OQQ48_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCS1952585.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGKNL"
     gene            complement(597..1478)
                     /gene="yddG"
                     /locus_tag="OQQ48_001784"
     CDS             complement(597..1478)
                     /gene="yddG"
                     /locus_tag="OQQ48_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HCS1952586.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEAGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTQQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            1710..4757
                     /gene="fdnG"
                     /locus_tag="OQQ48_001785"
     CDS             1710..4757
                     /gene="fdnG"
                     /locus_tag="OQQ48_001785"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:2295..2297,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCS1952587.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIAVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLAGQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGERVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            4770..5654
                     /gene="fdnH"
                     /locus_tag="OQQ48_001786"
     CDS             4770..5654
                     /gene="fdnH"
                     /locus_tag="OQQ48_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HCS1952588.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            5647..6300
                     /gene="fdnI"
                     /locus_tag="OQQ48_001787"
     CDS             5647..6300
                     /gene="fdnI"
                     /locus_tag="OQQ48_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HCS1952589.1"
                     /translation="MSKSKMIVRTKFIDRVCHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(6351..6635)
                     /locus_tag="OQQ48_001788"
     CDS             complement(6351..6635)
                     /locus_tag="OQQ48_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCS1952590.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(6781..7791)
                     /gene="adhP"
                     /locus_tag="OQQ48_001789"
                     /pseudo
     CDS             complement(6781..7791)
                     /gene="adhP"
                     /locus_tag="OQQ48_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415995.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
     gene            complement(7925..9622)
                     /gene="maeA"
                     /locus_tag="OQQ48_001790"
     CDS             complement(7925..9622)
                     /gene="maeA"
                     /locus_tag="OQQ48_001790"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCS1952591.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     IDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            complement(9779..9916)
                     /gene="sra"
                     /locus_tag="OQQ48_001791"
     CDS             complement(9779..9916)
                     /gene="sra"
                     /locus_tag="OQQ48_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HCS1952592.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            complement(10018..10233)
                     /gene="bdm"
                     /locus_tag="OQQ48_001792"
     CDS             complement(10018..10233)
                     /gene="bdm"
                     /locus_tag="OQQ48_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HCS1952593.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            10579..11010
                     /gene="osmC"
                     /locus_tag="OQQ48_001793"
     CDS             10579..11010
                     /gene="osmC"
                     /locus_tag="OQQ48_001793"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HCS1952594.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSFMLGEAGFTPTSIDTIADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            complement(11066..11992)
                     /locus_tag="OQQ48_001794"
     CDS             complement(11066..11992)
                     /locus_tag="OQQ48_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS1952595.1"
                     /translation="MSDTLLTLRGVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLPVWRI
                     ITEPLWIAKRSSEQQRRALVEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGDAQQVLTAPAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFF
                     YERCPLATSGCEVRQSLAIREDGRELRCWRAL"
     gene            complement(11985..12971)
                     /locus_tag="OQQ48_001795"
     CDS             complement(11985..12971)
                     /locus_tag="OQQ48_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS1952596.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIVLLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVRADVIHHPRHPYTIGLLQCAPEHGVPRQLLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISV"
     gene            complement(12968..13864)
                     /locus_tag="OQQ48_001796"
     CDS             complement(12968..13864)
                     /locus_tag="OQQ48_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HCS1952597.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLIWMLKGSPLTVTGAVIIVLML
                     LMMFFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLISWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            complement(13861..14883)
                     /locus_tag="OQQ48_001797"
     CDS             complement(13861..14883)
                     /locus_tag="OQQ48_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1952598.1"
                     /translation="MTFWSILRQRCWGLVLVMAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYVSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            complement(14885..16435)
                     /locus_tag="OQQ48_001798"
     CDS             complement(14885..16435)
                     /locus_tag="OQQ48_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCS1952599.1"
                     /translation="MKRSIPFRPTLLALVLATTFPVAHAAVPKDMLVIGKAADPQTLA
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDAPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDKDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            complement(16449..17030)
                     /gene="ddpX"
                     /locus_tag="OQQ48_001799"
     CDS             complement(16449..17030)
                     /gene="ddpX"
                     /locus_tag="OQQ48_001799"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HCS1952600.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFTCFISPGTQHVS"
     gene            complement(17288..19687)
                     /gene="dosP"
                     /locus_tag="OQQ48_001800"
                     /gene_synonym="pdeO"
     CDS             complement(17288..19687)
                     /gene="dosP"
                     /locus_tag="OQQ48_001800"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase"
                     /protein_id="HCS1952601.1"
                     /translation="MKLTDAENAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFRGKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNHDYL
                     LSTAHNAIDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMHAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            complement(19712..21093)
                     /gene="dosC"
                     /locus_tag="OQQ48_001801"
                     /pseudo
     CDS             complement(19712..21093)
                     /gene="dosC"
                     /locus_tag="OQQ48_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
     gene            complement(21465..22784)
                     /locus_tag="OQQ48_001802"
     CDS             complement(21465..22784)
                     /locus_tag="OQQ48_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HCS1952602.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIT
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            complement(22915..24450)
                     /gene="gadC"
                     /locus_tag="OQQ48_001803"
     CDS             complement(22915..24450)
                     /gene="gadC"
                     /locus_tag="OQQ48_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HCS1952603.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
BASE COUNT         6179 a         6230 c         6226 g         6105 t
ORIGIN      
        1 gtgtaatata aggtcagtga tcatattgtg atgggcactt aactcaatca gagattcttg
       61 ccgcgcgagc cgttggcttc atcgttatag cctttaaaat catattccca gtgaccaaaa
      121 ccaatcattt gatcattgat ttgcgtttct cctttaaagc cgagacgggc ataagtttta
      181 tcaccatcgt cacgcttatc atcagtaaaa taacgtagag cggtaacctt gccataaagg
      241 tcgagtttat taccatcctt gttatagact tcggcagcgt gtgctacgtt cgcagctaac
      301 aaaccagtta caaccaccgc aactattttt aatttcattt aacagaatcc ttttaattat
      361 cgtcaaacgt attttctaaa cgaattttaa acggcgtcat ttataaatga catactgttt
      421 ttaaaataga atggtgatta aaaacaatga gaaaaataga tgagataata ttatttatcg
      481 atatgtgatc gaagtcgaaa tgagatataa ggtgaattac tggtatttga aatttatttt
      541 ttaatattgt cggaatttat ctgattaact gccgggccgt agacccggca attattttaa
      601 ccacgacgtg tcgccagcca gcagagcagg gaaccgccgc agaccattag cgcgccttgc
      661 cagaatgaga acgacagcgg ggcgctgagc agcacggctg caagcgctga ggaaagtacg
      721 ggcgtaaaat acgagcctac cgccattatg gtgacattgc catgcaatat accgacattc
      781 catgcagcat aagcaaatcc taaggtaaat gccgcagaga tgagtttaat catgacgggc
      841 gtgctaaata ccatttctgg ttgttgcgta agaaaatagt aaacccacag acttgctccc
      901 gttagcagga caaaaacggt aattccgtta aatccgcgtg cgtatttatt cgttactgtg
      961 caataggctg cccagataaa cgcaccaata aacgccagga aataactcaa tgggctggtg
     1021 gtgatattat tgatgatttc atcataatgt aacccattct caccgcctaa cacccagcag
     1081 acgccgacca gggctaataa taatcctggt acaatcagcc agttggtttt ctgaccatta
     1141 aacagaatgg caaagagaat tgtcaggctg ggccacagat agttcaccat acccgcttca
     1201 atcgcctgat gacgggtcgc cgcataccct aaggaaagcg ccagacagat ttcatagctg
     1261 acgaataaca gactcccggc gagtaaatag cctttcggaa tctgccgaat acgcggaaat
     1321 ccaaccgtga agattaacag cagcccgctt aatgaataga tagcagctgc gccgccgacc
     1381 gggccgagcc cctcgctgac accgcgaatc aatcctacca tcgtgctcca caggacgatc
     1441 gctattagcc ctatgagcgt tgctttttgt cgtgtcatgc tcgctgtttt gtctctcttg
     1501 ccgttaaaaa ttaagctgaa ttttatagca tttttttaac tggcctgtca ggcagtggtg
     1561 cgtttttcta ccgctattga ggtaggtcaa tttgcgcagg cggattattt tgtggcaaac
     1621 acgtgttctt tttgatttcg cgcaaaaaga ttcagaattt tactgttagt ttcctcgcgc
     1681 agtaatatcc ctgaaaaaag aggaaagcaa tggacgtcag tcgcagacaa ttttttaaaa
     1741 tctgcgcggg cggtatggct ggaacaacgg tagcggcatt gggctttgcc cctaagcaag
     1801 cactggctca ggcgcgaaac tacaaattat tacgcgctaa agagatccgt aacacctgca
     1861 catactgttc cgtaggttgc gggctattga tgtatagcct gggtgatggc gcgaaaaacg
     1921 ccagagaagc gatttatcac attgaaggtg acccggatca tccggtaagc cgtggtgcgc
     1981 tgtgcccaaa aggggccggt ttgctggatt acgtcaacag cgaaaaccgt ctgcgctacc
     2041 cggaatatcg tgcgccaggt tctgacaaat ggcagcgcat tagctgggaa gaagcattct
     2101 cccgtattgc aaagctgatg aaagctgacc gtgacgctaa ctttattgaa aagaacgagc
     2161 agggcgtaac ggtaaaccgt tggctttcta ccggtatgct gtgtgcctcc ggtgccagca
     2221 acgaaaccgg gatgctgaca cagaaatttg cccgctccct cgggatgctg gcggtagaca
     2281 accaggcgcg cgtctgacac ggaccaacgg tagcaagtct tgctccaaca tttggtcgcg
     2341 gtgcgatgac caaccactgg gtggatatca aaaacgctaa cgtcgtaatg gtaatgggcg
     2401 gtaacgctgc tgaagcgcat cccgtcggtt tccgctgggc gatggaagcg aaaaacaaca
     2461 acgatgcaac cttgatcgct gtcgatcctc gttttacgcg taccgcttct gtggcggata
     2521 tttacgcacc tattcgttcc ggtacggaca ttacgttcct gtctggcgtt ttgcgctacc
     2581 tgatcgaaaa caacaaaatc aacgccgaat acgttaaaca ttacaccaac gccagcctgc
     2641 tggtgcgtga tgattttgct ttcgaagacg gcctgttcag cggctacgac gctgaaaaac
     2701 gccagtatga taagtcgtcc tggaactatc agttcgatga aaacggctat gcaaaacgcg
     2761 atgaaacact gactcatccg cgctgtgtgt ggaacctgct gaaagcgcac gtttcccgct
     2821 acacgccgga cgtcgttgaa aacatctgcg gtacgccaaa agccgacttc ctgaaagtgt
     2881 gtgaagtgct ggcctccacc agcgcaccgg atcgcacaac caccttcctg tacgcgctgg
     2941 gctggacgca gcacaccgtg ggtgcgcaga acatccgtac tatggcgatg atccagttgc
     3001 tgctcggtaa catgggtatg gccggtggcg gcgtgaacgc attgcgtggt cactccaaca
     3061 ttcaggggct gactgactta ggtctgctct ctaccagcct gccaggttat ctgacgcttc
     3121 cgtcagaaaa acaggttgat ttgcagtcgt atctggaagc gaacacgccg aaagcgacgc
     3181 tggctggtca ggtgaactac tggagcaact atccgaagtt cttcgttagc ctgatgaaat
     3241 ctttctatgg cgatgccgcg cagaaagaga acaactgggg ctatgactgg ctgccgaagt
     3301 gggaccagac ctacgacgtc atcaagtatt tcaacatgat ggacgaaggc aaagtcaccg
     3361 gttatttctg ccagggcttt aacccggttg cgtccttccc ggacaaaaac aaagtggtga
     3421 gctgcctgag caagctgaag tacatggtgg ttatcgatcc gctggtgact gaaacctcta
     3481 ccttctggca gaaccacggc gagtcgaacg atgtcgatcc ggcgtctatt cagactgaag
     3541 tattccgtct gccttcgacc tgctttgccg aagaagatgg ttctatcgct aactccggtc
     3601 gctggttgca gtggcactgg aaaggtcagg acgcgccggg cgaagcgcgt aacgacggcg
     3661 aaattctggc gggtatctac catcatctgc gcgagctgta ccaggccgaa ggtggtaaag
     3721 gcgtagaacc gctgatgaag atgagctgga actacaagca gccgcacgaa ccgcaatctg
     3781 acgaagtggc taaagagaac aacggctacg cgctggaaga tctctatgac gctaatggcg
     3841 tgcttattgc gaagaaaggt cagttgctga gtagctttgc gcatctgcgt gatgacggta
     3901 caaccgcatc ttcttgctgg atctacaccg gtagctggac agagcagggc aaccagatgg
     3961 ctaaccgcga taactccgac ccgtccggtc tggggaatac gctgggatgg gcctgggcgt
     4021 ggccgctcaa ccgtcgcgtg ctgtacaacc gtgcttcggc ggatatcaac ggtaaaccgt
     4081 gggatccgaa acggatgctg atccagtgga acggcagcaa gtggacgggt aacgatattc
     4141 ctgacttcgg caatgccgca ccgggtacgc caaccgggcc gtttatcatg cagccggaag
     4201 ggatgggacg cctgtttgcc atcaacaaaa tggcggaagg tccgttcccg gaacactacg
     4261 agccgattga aacgccgctg ggcactaacc cgctgcatcc gaacgtggtg tctaacccgg
     4321 ttgttcgtct gtatgaacaa gacgcgctgc ggatgggtaa aaaagagcag ttcccgtatg
     4381 tgggtacgac ctatcgtctg accgagcact tccacacctg gaccaagcac gcattgctca
     4441 acgcaattgc tcagccggaa cagtttgtgg aaatcagcga aacgctggcg gcggcgaaag
     4501 gcattaataa tggcgagcgt gtcactgtct ccagcaagcg tggctttatc cgcgcggtgg
     4561 ctgtggtaac gcgtcgtctg aaaccgctga atgtaaatgg tcagcaggtt gaaacggtgg
     4621 gtattccaat ccactggggc tttgagggtg tcgcgcgtaa aggttatatc gctaacactc
     4681 tgacgccgaa tgtcggtgat gcaaactcgc aaacgccgga atataaagcg ttcttagtca
     4741 acatcgagaa ggcgtaaggg ggcgaacaga tggctatgga aacgcaggac attatcaaaa
     4801 ggtccgcaac taactccatc acgccgcctt ctcaggtgcg tgattacaaa gcagaagtcg
     4861 caaaacttat cgacgtttcc acctgtatcg gctgtaaagc ctgtcaggtg gcgtgttcgg
     4921 agtggaacga catccgtgat gaagtggggc actgcgtcgg ggtttacgat aaccccgccg
     4981 atctgagcgc caagtcctgg acggtgatgc gctttagcga aaccgaacag aacggcaagc
     5041 tggagtggct gatccgtaaa gacggctgta tgcactgtga agatcccggc tgcctgaagg
     5101 cgtgcccgtc tgctggtgca atcattcagt acgctaacgg gattgtcgat ttccagtcgg
     5161 aaaactgcat cggctgtggt tactgcattg ccgggtgtcc gtttaatatt ccgcgcctca
     5221 acaaagagga taaccgggta tataaatgca cgctctgcgt cgatcgcgtc agcgtcggcc
     5281 aggaaccggc ttgtgtgaaa acctgtccga ccggggctat ccacttcggc accaagaagg
     5341 agatgctgga gctggcggaa cagcgcgtgg cgaaactgaa agcgcgtggt tacgaacatg
     5401 ctggcgtcta caacccggaa ggggtcggtg gtacgcacgt tatgtacgtg ctgcatcacg
     5461 ccgatcagcc ggagctgtat cacggtctgc cgaaagatcc gaagatcgac acctcggtaa
     5521 gcctgtggaa aggcgcgttg aaaccgctgg cagcggctgg ctttattgcc acttttgccg
     5581 ggttgatttt ccactacatc ggtattggcc cgaataagga agtggacgat gacgaggagg
     5641 atcatcatga gtaagtcgaa aatgattgtg cgcaccaaat ttattgatcg cgtctgtcac
     5701 tggaccgtgg tgatttgctt cttcctggtg gcgctgtccg ggatttcgtt cttcttcccg
     5761 acgctgcaat ggctgacgca aaccttcggt acgccgcaga tgggacgcat tttgcacccg
     5821 ttcttcggca ttgcgatttt cgtcgcgctg atgtttatgt ttgtgcgttt tgtgcatcac
     5881 aacatcccgg ataagaaaga tattccgtgg ctattgaaca ttgtcgaagt attaaaaggc
     5941 aatgagcaca aagtggcgga tgttggtaag tacaacgccg ggcaaaagat gatgttctgg
     6001 tcgatcatga gcatgatttt cgtgctgttg gtgaccgggg tgattatctg gcgtccgtac
     6061 tttgcgcagt acttcccgat gcaggttgtt cgctacagcc tgctgatcca cgcggctgcc
     6121 ggtatcatcc tgatccacgc catcctgatc catatgtata tggcattctg ggtgaaagga
     6181 tcgattaaag ggatgatcga agggaaggta agccgtcgct gggcgaagaa acaccatccg
     6241 cgctggtatc gtgaaatcga gaaggcagaa gcgaaaaaag agagtgaaga agggatataa
     6301 tctcttttga actttaagct gaaaatggcg ctttaagaag cgccattttt ttattgcgta
     6361 accagacggc gcagtcgcga cacatccacc gttttttcag cctctgccag actccaggcg
     6421 ttttgcagat tcagccacat ttgcggcgaa ctgccgatca ccacggaaag tttaattgcc
     6481 atttctggcg tcaaagctgc ttttccggtc agcaatcggc ttgccgttga gggcgcaatt
     6541 tccattgctc tggcaaactc gcgcaggctg acattaagtt cgtccagtga ttcctgaata
     6601 atgtcccccg ggcggggatg attggccatt ttcattagtg tctttactct cagtagcgtt
     6661 ccttctctgg gaacactatc attattgttc cttcctacgc aacaataatc catttagcca
     6721 tttcatgttg gagagtgtgc gatgctgcga cccgaacatg ggagtcgcag caaagacctc
     6781 ttagtgacgg aaatcaatca ccatacggcc acggattttg ccttcttcca tctcggtaaa
     6841 gatggtgttg atgtccgcta acggacgcag ggcgactttc ggcaccactt tgccttcggc
     6901 ggcaaactgg aaggcttcgg ttagatcctg gcgcgtgccg accagcgaac cgaccacttc
     6961 aatgccatcc agcacaagac gtgggatatc caggctcata gactccggcg gcagaccgac
     7021 agccacaaca cgaccgcctg cacggacagc atcaactgcc gagttaaacg cagctttagc
     7081 tactgctgtt accaccgcag cgtgagcgcc accggttttc tcctgcacaa tttaggcggc
     7141 gtcttcggtg cgtgagttaa tcgctaaatc tgcacccatt tcggttgcca gttttaactg
     7201 ctcatcattg acatcaatgg cgatcacttt ggcgttaaag acattcttcg cgtattgcag
     7261 ggcgaggtta cccagaccgc caagaccgta gatagcaatc cactgaccgg gacgaatttt
     7321 tgacagttta acggctttgt aggtggtgcc ccccgcacag gtaatgctgc tggccgccgc
     7381 cgagtccaga ccatctggca cttttaccgc gtaatcggcg accacgatgc actcttccgc
     7441 catcccgcca tcaacgctgt atccggcatt tttaactgaa cggcagagcg tttcgttacc
     7501 actgttacag tattcgcaat gaccgcatcc ttcgtaaaac cacgccacgc tggcacgatc
     7561 gcctggtttt aatgaggtga cacctgggcc aacttctgcc accacaccga tgccttcatg
     7621 gcccagaatt accccagttt tgtcaccaaa atcgccattc ttaacatgaa gatcggtatg
     7681 acatacacca caacactcca ttttcagcag ggcttcgcca tgtttcagtg agcgcagtgt
     7741 tttatccgta acgtcaacat gatgatcctt cgtaacaact gcagccttca tagttcctcc
     7801 ttttcggatg atgttctgca tagcaggtga ggcaaatgag atttattcgc cactacccag
     7861 tatggatgag atctgaaaaa gggagaggga aatagcccgg tagccttcac taccgggcgc
     7921 aggcttagat ggaggtacgg cggtagtcgc ggtattcggc ttgccagaaa ttatcgtcaa
     7981 tggcttgttg caaagcttca gcagacgttt tcaccgccac gccttgctgc tgcgccattt
     8041 tgccaaccgc aaacgcaatt gcgcgggaga ctttctgaat atctttcagt tccggtagta
     8101 ccagaccttc gccgttcagc accagcggcg aatactgagc aagcgtttca cttgctgaca
     8161 tcagcatctc atcggtgata cgtgacgcgc cggaagcaat aacacccagc ccgatgccag
     8221 ggaaaataaa ggcgttgtta cactgggcga tagggtagat tttatctttc cataccactg
     8281 gattaaacgg gctgccagtg gcgaccagcg cgttaccttc ggtccaggcg ataatgtcct
     8341 gcggtgtggc ttccacgcgt gacgtcgggt tagacagcgg catcacgatc ggacgcggac
     8401 agtgtttatg catctcacgg atgatctctt ccgtaaacag cccggtctgt cctgagacgc
     8461 caatcagaat atctggtttt acattgcgca ccacatccag cagtgacagc acatcgctgt
     8521 cggtatccca gtcactgagg ttttcgcgtt tctgcaccag tttggtctgg aaaggcagca
     8581 ggttcggcat cttgtccgtc agcaggccaa agcgatcgac cataaagact ttctgccgcg
     8641 ccgcttcctc gcttaatcct tcgcgctggg tctgggcgat gatcatttcg gcaatgccgc
     8701 atcccgctga acctgcacca aggaagacga tttttttctc gcttaactga ccacctgccg
     8761 ctcggcttgc tgcgatcagt gtgccgactg ttaccgccgc agtgccctga atgtcatcgt
     8821 taaaagaaca aatttcattg cgatagcggt taagtaacgg catcgcattt ttttgagcaa
     8881 agtcttcaaa ctgcaacagc acgtctggcc agcgttgttt cacagcctgg ataaattcat
     8941 caacgaattc atagtattcg tcgtcaatga tacgcggatt acgccagccc atatacagcg
     9001 gatcgttaag cagctgttgg ttgttcgttc cgacatccag caccaccgga agggtatacg
     9061 ccgggctgat gccgccacag gcggtataga gcgacagttt accgatcgga atgcccatcc
     9121 cgccgatgcc ctggtcacca agccccagaa tacgttcacc gtcagtcacc acaatcactt
     9181 tgatattatg gttcggtacg ttttgcagaa tatcgtccat attgtggcgg ttctggtaag
     9241 agataaacac gccgcgtgaa cggcggtaga tctcagaaaa acgctcacag gctgcgccga
     9301 cggttggggt ataaataaca ggcatcatct catcaagatg attgtttacc agacggtaga
     9361 agagggtttc gttggtgtcc tggatgttac gcaggtagat gtgtttgtcg atttcggttt
     9421 tgaatccctg atactggatc catgctcgtt ccgcttgttc ttcgatggtt tcgaccactt
     9481 ccggcagtaa ccccagcagg ttgaagttac ggcgttcttc catgctgaag gcactgcctt
     9541 tattcaacaa cggaaattcc agcagtacag ggccagcgta agggatataa agcgaacgct
     9601 gtttttttgt ttttggttcc atgtcactca ctcttttttg aatatccatc cctggcacgc
     9661 ttttatcgtc tttgctttac cgccagggcg tcggccccaa gtataaagca gataaaaaca
     9721 aaaacaccat tgcgcaggca atggtgttta atcgtcattg aggactgatg gttatgaatt
     9781 acttttcagc ggggcgtttt ctgccggttg ggttatttac cacgctggat ttgtcacctt
     9841 cggtaactat tttgcgctgg ttagaaattt tatggtccag tccaagaata tgacgtgcct
     9901 gacggttcga tttcatttta actcctcaat cctgttgcta gttttaagga caacatcgcc
     9961 gtagcgaaga aacacgtgct aaacccctaa attaggttgc cgatcaagca tagcacctta
    10021 aagcgtaggg tgctggccac tgaccacata attgatcgtt tgctggtaga tatcactgag
    10081 gatgtcgtta tcagaagctt ccacccattt ggtcagctcc atgagaatgt catcttcagt
    10141 gacaacaccg tgctctgccc gaaggccttg ctcaatggca ttaaccagag cgggttctgc
    10201 tgttgaattt tctgcctgat aataagtaaa catagtgatt ctccgtgtct gtgtatttat
    10261 ggtgtttgct acggatcgca gatttataaa gcacattcag catggcaaat atttgccgct
    10321 ttgttgttaa gattagtcct tgttgatgat ttttatattt taacctcatg atattgatag
    10381 gtattattat tgtggtgagt tgattaatat taatgcaata tctcttgggt tatatattcc
    10441 cggttatcta ttgtggaaat ttaatttaag tgcagaagta atatttctgc cggattttta
    10501 ttcggaatat cctgcttatc ctcgtgctgt ttctcacgta gtctataatt tcctttttaa
    10561 gcccacatga gagcaacaat gacaatccat aagaaaggtc aggcacactg ggaaggcgat
    10621 atcaaacgcg ggaagggaac agtatccacc gagagtggcg tgctgaacca acagccgtat
    10681 ggatttaaca cgcgttttga aggcgaaaaa ggaactaacc ctgaagaact gattggcgca
    10741 gcgcatgccg catgtttctc aatggcgctt tcattcatgc tgggggaagc gggatttacg
    10801 ccaacatcga ttgataccat cgccgatgtg tcgctggata aagtggatgc cggttttgcg
    10861 attacgaaaa tcgcactgaa gagtgaagtt gcggtgccgg gtattgatgc ctctaccttt
    10921 gacggcatca tccagaaagc aaaagcagga tgcccggtct ctcaggtact gaaagcggaa
    10981 attacgctgg attaccagtt gaaatcgtaa agcattgccg gatgacgtgt caggcgcgtg
    11041 aatgcctgat gcgttgttag catctctaca gcgcccgcca gcaccggagc tcacgtccgt
    11101 cctcccttat tgctaatgat tgccggactt cacatccgct ggttgcaagg gggcaacgtt
    11161 cgtagaaaaa acagccttgc ggcaacgtgc ggtttcccgg cagatccgtt ttacgtaatg
    11221 cccattcttc ctccagcggt ttatcaatgg cggggaggga atccagcaat aatcgggtgt
    11281 atggatgtgc cggagccgtt agcacctgtt gcgcgtcgcc cagttctaca atctgcccga
    11341 gatacatcac cgccacccga tcgctcatat gacgtatcac cgagacattg tgtgaaatca
    11401 gcacataggt cagcccgtga ttttcctgta gcgttaccag taaattgagg atctgcgcct
    11461 gcacggagat atccagcgca gaggttggct catcaagcac aatcacgtca ggctgcgaag
    11521 agagtgctct ggcaatggcg atacgttgcc gctgcccgcc ggagaacgca tgaggcaggc
    11581 ggtcgagata ctccggacga atacccacct gcacagccag ctcctctacc aacgctcgcc
    11641 gctgttgttc actactacgc ttagctatcc agagcggttc tgtgatgatg cgccacaccg
    11701 gtaagcgcgg attgagcgaa gagagcgggt cctgaaacac catctgcata atgcgttgtg
    11761 agccagaacg gatgtactgc ccgtggctcg gttgcagcat acccattaaa agctgtgcga
    11821 gggtgctttt gccgcagcct gactcgccga cgatccctaa ggtttcacca cggcggatct
    11881 gtaaatcaat accattaatc gcatgaacat gttccgtagt tttaccaagc cagtttttac
    11941 gggccgggaa attgatatgg acgccgcgta acgttaataa cgtgtcagac acttatgacc
    12001 tcctgctgcg gataccaaca ggcgcagcgc tggttgttgt caccacacgc tgtcagcgcc
    12061 gggacgtttt cacactgtgc acctgccgca tagcaacgat cgcgaaaagc gcagccgtca
    12121 ggcaaatggg tgaggtttgg taccgtccct ggaatggcgg gtaatagctg gcgtggtact
    12181 ccatgttctg gtgcacattg gagcaaacca atggtatacg gatgccgggg atgatggata
    12241 acgtctgccc tcacgccgct ttcaattacg cttccggcat acatcacgta aacgctatcg
    12301 cacagttgcg acaccacggc catatcatgg ctgatgaaca gaaccgcagt tccactggcg
    12361 cgggctttat gtttaagcaa acgcagtacc tgcaactgca ccgtgacgtc cagcgccgta
    12421 gtcggttcgt cggcaataat caattgcggc tcgcaggaga atgccagcgc aatcattacc
    12481 cgctggcgca taccacctga aagctcaaac ggatagcgcg acataacttc cacggcatcc
    12541 gggatttgca tctcttccag caggacaatc gctttagctc tggcttcccg acgacttatt
    12601 ggttgatgat ggcggatcac gtccatcatc tgaagaccta ttcgacgtgt cggattgagg
    12661 gcggtcatcg gttcctgaaa gatcatcgcc actcgtgcgc cgcgccactg acgaagctgt
    12721 ttttcccgag cgttaagaac atcgtctccc agcagtgaaa tctgcccccg atgtacgcaa
    12781 taactgcccg tcggtagcag acgcataatc agcattgcgg tgactgattt acctgagccg
    12841 gattctccca ccagaccgac aatttcaccg cggttaatct gcaaggacac attgttgagc
    12901 gcgtgaacat caccgttaaa accggggaaa ctcaaatgca gctgttgaat gtccagaacg
    12961 ggctgggtca tgactgcttt cctcctgctt tcgggtcaag cagatcgcga ataccatcac
    13021 caaagagatt aaacccaacg gcggtaagca aaatcgctgc ccctggaaat gcgcaatacc
    13081 accactgatc gagtacatag ttgcgaccat tcgctaccat cgcgccccat tcagcactcg
    13141 gttgttgagc acccaggcca ataaatccca acgtggcggc cattaaaatc gcgctaccga
    13201 tatccagcga tgcctgcacg atcagcggcg gcagggagtt acgtaaaata tgccagctga
    13261 tcagatgcca acgcgacgca ccaaaggttt tcgccgcctg aacataggta tactggcgta
    13321 caactaatgc ttgcccccgc gccaggcgca cataaaaggg aattcgcaca atagcaatcg
    13381 ccagcatggc gttaaataaa ctcggcccga gagcggcagc cagtgccatt gtcagtacca
    13441 acgaaggaat cgacagcata atgtccatga tgcgcatgat gatggcgtct gcgcgtccgc
    13501 caagcacacc ggatagacat ccgagtagcg aaccaatcat acccgcaatg gcaaccacga
    13561 ctaatccggc gagaattgat tgctgactgc cgaccagtac gcggctgaac agatcgcgtc
    13621 ccacttcatc ggtgccaaac cagtgcgccg cagaaggcgg caaaaggcgg gcggttaaat
    13681 caatggcgtt gggatcatgc gtcgcaagcc acggtgaaaa aaacatcatc aatagcatta
    13741 atacaatgat gactgcgccg gtcacggtga gcggactgcc tttcagcatc catatcagtt
    13801 ttgcaccgtt aaatcgcgtt tgtttttgtg ggcgtacggc ggacgtttcc tcgcttagca
    13861 tcattcacca cctccacgtc cgatacgcgg atcaatccac aaatagagca aatccaccac
    13921 caggttgacc agcacataag caaatgaaac cacgacggca aagcccatca ctgccgggaa
    13981 gtcgagcgcc tgtattgatg ttactaccca tgcacccatt ccgggccagg caaaaacggt
    14041 ttcggtgagc actgcgccat acaacaaatc gcccagcgcc agacccaata cggtaatcga
    14101 tgggatcaac gcattgggta gcgcataaca taaaacgata taccagccgg gcaagccgct
    14161 ggcccgggcg gtacgaatgt agtcttcact caattgttcc agcatcgctg agcggatctg
    14221 gcgagcgaca attcccaggt gaacgaacgc cagcgttaat gccggtaaga tgagatgttg
    14281 caacgcattg aagaagactt caccgttgcc ttcaagcagc gcatcgagca gataaaagcc
    14341 ggtaacgtgc gttggtggat ccagccagtc atcaagcctt ccgccgccgg gaagaatttg
    14401 cagatgacca taaaacagca caatgacgcc cagtccaagc caaaacgcag gtgtggagat
    14461 tccggtaatg gccattattc gcaccagatg atccagccag cgatttcgcc agactgcaga
    14521 gagtatgccc agcgggatgc caatcaggag tgccagcagc agggcacaaa aagccagttc
    14581 cagcgtcgcc gggaaaaata tacgcaactc ttccaacacc ggacgcccgg tacgaatgga
    14641 tgttcccagg tcaccatgaa acaggtcgct gacgtagcgg taaaactgta catacagtgg
    14701 ctggtccagt cccagttgct ggcgaatatt ttccacgata gcatcgctgg cgcggtcacc
    14761 cgccagtaac cgcgccggat cgccagggat cagatgcgag ataataaacg taatcacgca
    14821 gacgcccgcc atcacgagca ccagccccca gcagcgttgg cgtaaaatac tccagaaggt
    14881 cacgttattt actcatggta ttgatattga agacctgttc cagcatggga ttgaacacaa
    14941 agcctttcac ctctttgttc atcgccagtt ggtagttttt ctggaacagg tacacataag
    15001 cagcgtcatc aatgacgatt ttctgtgcct gctggtagtc ccgggtacgc tgcgtctggt
    15061 cggtggtcgc aagcgcattg cgcagtaact tatcgacctc actgttttca tagaacgagc
    15121 ggttacccgg cagacctttt ttgtctgact caaaccagta attcataaac atatacgggt
    15181 cggcaaaatc cggactccag ttgccaatcg caatgtcgta atcaccttta cccactctgt
    15241 cgcgcatggt ggcgttcgcc agcttttcca gcttcacatt gatgcccagc ttgttgagac
    15301 tggattgtgt cgccagagca ataggctccc agttcggatc cttatcggag tagagaaacg
    15361 tcaggctggt gggtttgctc gtcactttat cccattcggc tttggctttc gtttcgtcat
    15421 ggttgtattg cattgccgtc gcatcgtagc cccacatgcc ttccggaatc gggccgcgca
    15481 tctgttttcc gttaccactc agaatgccgt taaccatgcc ctgataatcg gtagaccagg
    15541 aaatggcccg acgcagatcc gcctgattaa gaggcgcttt gctgttattc agatacaggt
    15601 aggtgacgcg cagtgacgga tactctgcca cattgacttt gttttcctgc ttcagggcgt
    15661 tgagttgatc caccggcagc gcatcggcaa tgtcaatatc accacgggag agctgcaggc
    15721 gacgggaggc actttcacca ataattttta ccgatactcg cttaaagttc ggtttattgc
    15781 cagggtaatg cggatttggc accagaacta attgctgacc tttttgccag cttttcagca
    15841 taaatggtcc ggaaccggcg gtattttgcg cgaggaagcc gcgggcatca tccgctgcat
    15901 gttcctttaa caccgccgga ttgataatgg atgcaccgtc attcgccagc gtgtagagga
    15961 acggtgcgaa tggctggcta agggtaaact tcaccgtatg ttcgtcggga gcatcaatct
    16021 ttaaatcttt gggaaatgct tctgctggcc cctggccgat ttttagtagc cgctcaaaag
    16081 agagttttac tgcttcggca gtgaccggtg tgccatcggc aaatttagca ttgtccttca
    16141 gggtgaacgt ccactctttt tgatcgtcag acgctttcca gctacttgcc agatcgcctt
    16201 caacgtcggt tgagccttta tcaccgtccg ttttgtactg aaccagccgc tgataagacg
    16261 ggtaggtcac cgtccagtcg ttattatcta ttgttaccgc cggggcgagg gtttgtggat
    16321 cggcggcctt accaatcacc agcatatctt tcggtacggc ggcgtgcgca accgggaaag
    16381 ttgtggcaag gacgagcgcg agcaatgtag gacgaaacgg tatcgatctc ttcatgacag
    16441 tttcctgatt aactgacgtg ctgtgtgcca ggggatataa aacaggtgaa ttgatcagcg
    16501 agcagtgggt aactcgctgc ctggggtaat tcgaagtgcc accattcgct ggagataccg
    16561 acaaaaccgc caccagtcat aatcgcattc agcaacagcc gattgcgctg agcggcgggc
    16621 gggacggaag ggtgataggc atgggagcgc tcgtgcattt catcgaaccc tgcgcccata
    16681 tcgaggatgt tcccgtgctc atcacgaagc gtcaggtcga tcgccgtgcc acggctgtga
    16741 ttagaaccga ccgtcacatc aacaacatat tgcgggtctg ggcaggcttg ccacaacatc
    16801 gcctgtgctt gttgtgggcg atacgcatcg taaatcacca gttgtaagcc tgacagctgg
    16861 gcgatactga tacttttcgc cagcgcggta atcgcatcct tgtgtagcag acaacgcgct
    16921 tgctgataaa tagctttacc tgtgatgtta tcagcgcagg cgtatttcaa ttcgatctcc
    16981 agatcaggga agattacggc taaatcaacc agttcggtgg tatccgacat aaacggttcc
    17041 tgtataagac aaaaattgct gcgctttccg cttatgcaga tctcatgcca tgccgggata
    17101 agcgccagaa tgctggctta aagttatatt ttttaacttt tgatcaacat ttgtgcagcg
    17161 tagtgcagtt ttggtgcaag aggggaagtt aaggaaggaa tctcccggaa tcgtagatga
    17221 aatcacagta tttaagtgac ggtgtcacgt taaatgaaaa cccgcgagtg cgggcgagag
    17281 gaatttgtca gattttcagc ggtaacacgc tgctcatcca gcctggaatt tcttcggcgg
    17341 gtagggggcg ggaaaagaaa tatccctgaa taacgcgaca gtggatcttg cgtagcatct
    17401 caaattgctc tttggtttcg acgccttccg ccacgacggt taaattgagg ctttgcccaa
    17461 tgctggtaat ggcttcaagt aaggcaagga tgcgtttttc ggtcagacaa cgatcgacaa
    17521 aacttttgtc aattttgatt tccgttaccg gaagactgac taagcgggat aatccggaaa
    17581 agcccgtacc aaaatcatct accgataagc ccacgcccat atcacgcagg atctgaatgc
    17641 gcttaaagat ttcggtatcg tgttccatca tcatgctttc cgtgatttct accgtcagct
    17701 ggtggccgtc aataccccag gcgtgcattg catcagacac ctgattaggc agttgattac
    17761 tgcgaaagtg cagtgccgac aagttaacgg aaagcgccgg gatatgaata ttctggctac
    17821 gccattctgc taactgacgg caagcttccg cgatgaccca gcgcccaata ttttcgattt
    17881 caccaatctc ttctgcgaga ggaataaacc gtgaaggggg cacatgacca tgctggggat
    17941 cgtgccagcg cgcaagggct tcgatgccgt acagttcacc cgtttctgcg aagatttgcg
    18001 gctggtaaac aagtttcagt tggttattgc taatcgcttc tttcagcgct gcgcctaaaa
    18061 ccaaacgctc ttttaccatt tcgttcatcg ccgggctgaa gaactgccag ccgttaccgc
    18121 cattcttgcg aatataatct attgcattgt gagcagtgga gagtaggtaa tcgtggtttt
    18181 tacccacgtc gtagctgatg ccaatactca aggtaagcgg gaatggttta tcgtcaatca
    18241 ttatcggctt gctgaccaca ttccgtagct catcggcgat ctgggtaatg ttactgacgt
    18301 cgttttccag gctcacgagg acaaactgcg taccttcgat acgacagaga tactgatccg
    18361 gtttgagttt tccacgaaag cgattgacca cttccagcaa tgcctgatcg gcccacgcat
    18421 agccaaggct atcaatcaca tcctgaatat ggtcaacacc gatgagatac accaccggag
    18481 agacggcttt gtcgaccagg tcatcgaggt aattgtgcag gttattgcga tttggcagac
    18541 cggtcatcgg atcaaattgg atgagttgtt caatatgctg gcggcttttt tcctgttcca
    18601 gcgccagcgc ggccatatgc tgactgatat ctgccacgcg ttcgataaag gcgctggttt
    18661 ctgctcctga cgaggtttta atttgcagga tccccgcagg cgcgccatca cgctgacgaa
    18721 tggtcgctga ccagctttgc gcattttgaa cttctgcacc gtgcgaagat gacgcccagt
    18781 gtatcggcat cccgttgcgc agtgcgaaca gcgaaacatg cgattcgttg agaacagatt
    18841 cgatgttacg acaaatgatt tcccccattt catgaaatgg cgggctgctg cacatggcgg
    18901 cgagaatatt gccttcaagc tggcggattt gccgttcttc ggtgatatcc gagaaagtca
    18961 ttaccaggtt ctgcagatgc gcgagcacgt cataaaccgg gctgatagag gctttaatcc
    19021 agattttttc accggtgcgc gtcaacagca gaaattcgtc ctgatcgcgg gcggttttcc
    19081 atagcaactg ttgtaaacga atgcggttat cggcagggaa ttcaggaatg ttcaggagtg
    19141 tatcgggctg cataccgctg gcttcgctaa tgcagtaacc aaacatttcg gtaaatgcgc
    19201 gattgcactg cacaatatgg cgttccggat cgaggacaat caccggtcgg tcgagatggt
    19261 caacggcaat aatcaattgt cgggtctgtt ctttttgcgc catttctacg ctggcatccc
    19321 gtaccagcgc caggtaataa actttcccct cggcgctcac tttcgatagc gcaaaacggg
    19381 tccagatttt actgccgtct tttttctcca gctgcagctc ccgactcatc ccctcgacgc
    19441 gcgctttacc accttcacgg ttatgacgaa tgtattcagg atgcgcagga cgcaaatccc
    19501 gcggaatcag catatcaatg ttattgccaa tgacttcttc acgtttgtac ccccagagct
    19561 tctctgcggc ggggttgaaa aacatcactt catcattttc gttaattaac accgcgccca
    19621 tcatattttg ttcaagggcg gggaaaaaaa tgccatcggc ggcattttcc gcatcggtta
    19681 gcttcatgat tacctctgca tcctggcgca tctaaagact ggctttccag agttcaacac
    19741 ggtttctacc tcgtcttttg gcgatataca gagcttcatc ggctatttga atgaggcgct
    19801 catagtcagg atgaccatta aacatggcgg caccgatgga aagtgagagg gcaatatcct
    19861 cgccgtttgc ggctttcagt ttggttttct ccacccgact gcgaatacgt tctgcggtac
    19921 gtaacgtttc gttttcagaa gcttcagtca aaacaatgat aaattcatcg cccccgtagc
    19981 gaaaaacata atcactactg cggacgttgt cataaaaggc ctgagagact ttacgcagaa
    20041 tttcatcgcc agtgttatgg ccccacgtat cgttgatctc tttgaattta tcaacgtcaa
    20101 taatcagcac tgacagcggt gtaccggtcc ggttggcatg ggcaatttcg cgtttgaaga
    20161 tagtcggcag gaagcggcgg ttaagtaatt tcgtcagtac atccataccg acttcgtggc
    20221 gcgatacttc ttcaaacaat tcacgcagca aggtaataat ttgcgataca gtatttctta
    20281 tctgtaataa aaatttcacc cgcagacttc tgttattcaa attcctggtg ttacgcatgg
    20341 tttgattgaa tataccgtcg aaatcctgaa tcagacggga gatatgccta cttcggcaat
    20401 gccactaaaa taatgtcgac ctttatggtt aaaccacagg ccaaaatcag cctggcttaa
    20461 aggcaaacta ctgcctaaat cagaatccag caggatttta tagataatat ctatttccca
    20521 tgaaagtatt gaggctattt gccgttcttt ttcttcttcg gcgttttcta gtaacgagaa
    20581 gatacgatag ttttcatctt cctttgaggc actactgtca ctaaaggtaa acgcgcgggt
    20641 catcacttcc atagcgatat caatactgtt aatcgagaaa tggtagacct gaagtttttc
    20701 tgcggcagaa taatccgaag agaagattac cggatagagg atttttttca gcacccgaaa
    20761 ccccatctcg acaatttcta ccggaattcc tatgcgggca tgcacttccg cgacggtatg
    20821 ctggatttgt attagccttt cgacatcgtc aacctgggca gaaagcacgt taataatcca
    20881 gcgttccatc gcacttttca actgccgctc aacttgttca ttactcaaga attcttcggc
    20941 atgcgggtcg atgcggacaa ttcgataaaa ctcgatactc agataatgag catgcgcaac
    21001 ggcaatttcc gcggctttag cacgaatgag tggatctgcc tgttcgacaa gtccggtcca
    21061 ctcatctttc attcttttaa aatacatctc cataattcac acccttataa ggctgggaaa
    21121 tcagacggaa tcaaaacgca acgtgcgaga tcgactaact gcaccatatt ctcctgaaat
    21181 atgaagatat actgaaaaga aataagcgac ttaggacagt ttcaatctac gctactgttc
    21241 ttcagaagag tatagcccat cgtaattatt tttcggtgac agcgaatatc gtatggtttt
    21301 tcatattcat acatttttat tagggattta tagctgttta actaagtgtg gttaattgcg
    21361 tggttaattt gacttatgta agcatgatta ctagtgggat atttcacaag gtgtaacaag
    21421 ccggtgggta aagcaccggc ttgttacaaa gtaagcatgg gagtttaact gccccaacga
    21481 ctttgcagat agctgaccgc ttgttgagtc tgcggtttat tcagatagtc ctcacggaac
    21541 aagatggtgc cgctaatttc tggtaccgca tcgttaagat cgagctgctt tttcagttcc
    21601 ggtacgccgc cgttaatcat ccagtctggc tctatctttg aaggttcacc cactttatag
    21661 aaggcgatac cgatatacag gcgggtcctg gtcggtttaa cgacatccgc ccaccatttt
    21721 gccaacacgt cataacgcgc ggcactccgt gagaacggcc agtaaatttg gggagcaatg
    21781 taatccagca atccttgttc cacccatcga cgggtgtcag cgtaggattc gtcataggct
    21841 gccgcgcctc gggtatcgga accgagcgga tcgtgtgatc ggttacgcca cacgcctgcc
    21901 gggctaacgc caaattcgac tcccggctta atgcttttaa tggtgtgcga tacttttgta
    21961 attaactgct gagtattgtt gcgccgccag tctgcttttg acgcaaatgc gcctccgtat
    22021 ttacggtacg tttcgttatc atttagccgt gaacccggtg actccgtata gaaatagtcg
    22081 tcaaactgca cgccatctac cggatagcgg gaaaccactt cggcgactat tgatgtgatc
    22141 cagtcctgaa cctcagggat gcccgggtcg aggacaaagc gatcgccaga cgttctgatc
    22201 cagtcacggt gttgcacata gacgctcgcc ggttgttgag acagagtgct attcagttcc
    22261 ctgatagtac cgggcttcgt attaaccgat acgcgatagg ggttaaacca ggcgtgtact
    22321 ttcatcccac gcttgtgggc ttcgtcgagc ataaattgca gcggatcgta acccggattt
    22381 tcaccaatct taccggtcat aagatcggac cacggcaaaa tttttgatgg ccacagggcg
    22441 gtaccgtccg gcttgacctg gaaaaagacc gtgtttatac cgagacgttg cagatgatcc
    22501 agtttgtcga tcatcgcctg ttgttgtaca cgggcccggc tggtggggtt actaatgtta
    22561 accgaggaaa ccggtggcca gtcgaggcga gacaccgtgg ccagccagat gccacgcatc
    22621 ggttgtgacg attgttgcgt cgtggctggt ggctttgaac ctgctggtgg tgtcaccatg
    22681 gactctggag gcgtgctttt acaactacac agtaaaagtg ccagtgcaac cagtatcgct
    22741 ggtcttctaa tcgttaattt cttgtttcgg gagcagatat ccatatgtgc tggtttccgg
    22801 taaacagatg tgcgctcatt ctcgtactta tcccggttaa gtcaatacga cagcaagcac
    22861 gaaaaaggga gcgatgaatt atcgctccct tgtcttataa ccattcagac atggttagtg
    22921 tttcttgtca ttcatcacaa tatagtgtgg tgaacgtgca cgcgggtgca ggaagaagtg
    22981 accttttggt gcgttctgac tgttgattgg ctccagagtg acgccggtat tcgctttgcc
    23041 tttacgatca tgaacagcat agagaataaa gggcagggca agtaccacca ggaaactaac
    23101 gaccagtaat tcaacataca tatcggtaga gtcaccctgg atgttatccg gcggcaggaa
    23161 ggaaacaata aacgccataa ttgaagtcag cagaccgaca attgccacga ccagtttcac
    23221 ccctttacca ccagggatat taaatgtgcg ttttaagtca ggatgtttaa gaaccaacac
    23281 aatgtagcca ataaacagca tgaaataagc acacagataa atcaccaccg tcagcgccag
    23341 tgcgatcagg aaggacatgt tgttaccgcc accggtattg gtgaggatga tcaacgcgat
    23401 agaagtaatc accagctgcg aaatgaccag cgttaccggt acgccatttt tgttcatttt
    23461 agcgaatgcc gctggcagca ggtttttctg cgctgtgaca tacatcccgc gagaaggacc
    23521 aacaatccag gaggcgattt ccgccagaac acccagcaac agcagtgcgg agatcacgcg
    23581 aaccgtccac tcaatttccg gtgccacatg ggacatcaga acggtaaagg tttgcattac
    23641 ccctgcggag aggttgattt cattacccgg aatgaccatc gcaatagata aaccgccaac
    23701 agagcttaag cagattgccg ccaccatcag cagtaacata gccagtggat agtcgcgccc
    23761 ggggttgctc atttcattga cgtgagttgc ggaagcttct acgcccatat aactcaaaat
    23821 gaaggcaaca aatacaacca gtgtgcccac tttagagaag tcagggaaga aggtcttcga
    23881 atccatttcg atagcaacgg gggcaccgga gtgcagataa atagccgcta atgcgatcaa
    23941 aataaatgca ggtaacagga taccggcgaa gaagccaact ttagcaattc gcgccgtgta
    24001 tttcgtgcca ccaaactgcg ttaatgccag cgcccaaaga atgatgagtg ctgcaatagt
    24061 tttggtaatg gggtcttcat tcagcgctgg ccatttcagg atgtaggaga gtgcccctaa
    24121 cacgaaatag agcatcggaa taaaaccaat ggcgatttgc agatagccaa atgagatcgc
    24181 tgcaaatccc catctcggcc ccagagtatt tgatacccag gcgaagacac caccttcttc
    24241 ccagccgtcg acggtggcca tttccgcagc acaaagtccc acgggaataa accataaaat
    24301 cccgcctaat agcaggaaga agactaatga aaagcccgat gttgcgaagg tagggtattc
    24361 ataaacagcc attaccatcg atgccgttat ggcaaagaat ccgagtaatg tgagctgctt
    24421 agctttacct gtctgtactg atgtagccat attatccccc taaaacggta ttcctgtcgg
    24481 aaccgcacct gttttgttct gaagcgtatt cagaacaata ttttccgttg ctaatgccag
    24541 tgaacagact ttggaaattg tcccgaaacg ggttcgtttc gggacaccgt taccgttaaa
    24601 cgttatcagg tatgtttaaa gctgttctgc tgggcaatac cctgcagttt cgggtgatcg
    24661 ctgagatatt tcagggaggc tttgtagtct tccagcaaca gttcagcaaa gtccatttcg
    24721 aagccgcgac gacacataat
//
LOCUS       DAJVUJ010000049        24210 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.049, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000049 DAJVUJ010000000
VERSION     DAJVUJ010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24210
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.049"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(<1..2152)
                     /locus_tag="OQQ48_001804"
     CDS             complement(<1..2152)
                     /locus_tag="OQQ48_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HCS1952604.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSSEEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVLHPAYGNVRIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKA"
     gene            complement(2263..3675)
                     /gene="ftsP"
                     /locus_tag="OQQ48_001805"
     CDS             complement(2263..3675)
                     /gene="ftsP"
                     /locus_tag="OQQ48_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HCS1952605.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDSRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(3750..4487)
                     /gene="plsC"
                     /locus_tag="OQQ48_001806"
                     /gene_synonym="parF"
     CDS             complement(3750..4487)
                     /gene="plsC"
                     /locus_tag="OQQ48_001806"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HCS1952606.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(4720..6978)
                     /gene="parC"
                     /locus_tag="OQQ48_001807"
     CDS             complement(4720..6978)
                     /gene="parC"
                     /locus_tag="OQQ48_001807"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCS1952607.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LVSERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(7524..8006)
                     /locus_tag="OQQ48_001808"
     CDS             complement(7524..8006)
                     /locus_tag="OQQ48_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HCS1952608.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPKAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTVWGIIRHWLPASGEKMRKAPILFHYTNLAEGMTEQRLETDVYVPLA
                     "
     gene            complement(8059..8451)
                     /gene="ygiW"
                     /locus_tag="OQQ48_001809"
     CDS             complement(8059..8451)
                     /gene="ygiW"
                     /locus_tag="OQQ48_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HCS1952609.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSRRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            8603..9262
                     /gene="qseB"
                     /locus_tag="OQQ48_001810"
     CDS             8603..9262
                     /gene="qseB"
                     /locus_tag="OQQ48_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HCS1952610.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLASEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            9259..10608
                     /gene="qseC"
                     /locus_tag="OQQ48_001811"
     CDS             9259..10608
                     /gene="qseC"
                     /locus_tag="OQQ48_001811"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HCS1952611.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDS
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNVQGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPSGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            10718..11299
                     /gene="mdaB"
                     /locus_tag="OQQ48_001812"
     CDS             10718..11299
                     /gene="mdaB"
                     /locus_tag="OQQ48_001812"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HCS1952612.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            11330..11644
                     /locus_tag="OQQ48_001813"
     CDS             11330..11644
                     /locus_tag="OQQ48_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCS1952613.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(11692..13584)
                     /gene="parE"
                     /locus_tag="OQQ48_001814"
     CDS             complement(11692..13584)
                     /gene="parE"
                     /locus_tag="OQQ48_001814"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCS1952614.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQIYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(13613..14194)
                     /gene="yqiA"
                     /locus_tag="OQQ48_001815"
     CDS             complement(13613..14194)
                     /gene="yqiA"
                     /locus_tag="OQQ48_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HCS1952615.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(14194..15021)
                     /gene="cpdA"
                     /locus_tag="OQQ48_001816"
     CDS             complement(14194..15021)
                     /gene="cpdA"
                     /locus_tag="OQQ48_001816"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HCS1952616.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(15046..15468)
                     /locus_tag="OQQ48_001817"
     CDS             complement(15046..15468)
                     /locus_tag="OQQ48_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HCS1952617.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTTVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(15469..16098)
                     /gene="nudF"
                     /locus_tag="OQQ48_001818"
     CDS             complement(15469..16098)
                     /gene="nudF"
                     /locus_tag="OQQ48_001818"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HCS1952618.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHSLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            16303..17784
                     /gene="tolC"
                     /locus_tag="OQQ48_001819"
     CDS             16303..17784
                     /gene="tolC"
                     /locus_tag="OQQ48_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HCS1952619.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSIVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            17932..18603
                     /locus_tag="OQQ48_001820"
     CDS             17932..18603
                     /locus_tag="OQQ48_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HCS1952620.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLASCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            18609..19769
                     /locus_tag="OQQ48_001821"
     CDS             18609..19769
                     /locus_tag="OQQ48_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCS1952621.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLPLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(19807..20595)
                     /gene="ygiD"
                     /locus_tag="OQQ48_001822"
     CDS             complement(19807..20595)
                     /gene="ygiD"
                     /locus_tag="OQQ48_001822"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HCS1952622.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPVLAQHLVELLAPVPVT
                     LDKEARGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGAVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            20738..21499
                     /gene="zupT"
                     /locus_tag="OQQ48_001823"
     CDS             20738..21499
                     /gene="zupT"
                     /locus_tag="OQQ48_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HCS1952623.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGISPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAVAGIMVALSVDELMP
                     LAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(21557..21727)
                     /locus_tag="OQQ48_001824"
     CDS             complement(21557..21727)
                     /locus_tag="OQQ48_001824"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1952624.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(21987..22640)
                     /gene="ribB"
                     /locus_tag="OQQ48_001825"
     CDS             complement(21987..22640)
                     /gene="ribB"
                     /locus_tag="OQQ48_001825"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HCS1952625.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(22744..22892)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            23014..23304
                     /gene="ubiK"
                     /locus_tag="OQQ48_001826"
     CDS             23014..23304
                     /gene="ubiK"
                     /locus_tag="OQQ48_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HCS1952626.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRMSELENRSTEIKKQPDPETLPPTL"
     gene            23588..24139
                     /locus_tag="OQQ48_001827"
     CDS             23588..24139
                     /locus_tag="OQQ48_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HCS1952627.1"
                     /translation="MSAFKKSLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIK
                     PGDEDLTVNLGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTM
                     TGQTDLLKNTKETEIGGATGVGVRILNSQSGEVTLGTPVVITFNNTNSYQELNFKVRM
                     ESPGKDATPGNVYAQADYKIAYE"
BASE COUNT         5982 a         6457 c         6058 g         5713 t
ORIGIN      
        1 acgctttttt tgccgtagca ggcgtctgac gctgggtcgc catcggcgta tgtttcgtca
       61 acgccggacg ggtattgcgg ttctggcgac gagcttcacg catctcttca atggttggcg
      121 caggcactaa gcaatcgcga cggctaccaa tcagatgctt tttacccatc gcttccagcg
      181 cctggcggat taacggccag tttgccggat cgtggtaacg caacaacgct ttatgcaaac
      241 gacgctgctt gtcgcccttc ggtacgaaga cgtcttcact cttataacca atcttcgcca
      301 gcggtttttt cccggtgtaa tacatggtgg ttgagttcgc cagcggcgat gggtagaagt
      361 tctgtacctg gtcgagacgg aaacgatgct ttttcagcca cagcgccaga ttcaccatat
      421 cttcatcacg cgtaccgggg tgcgcggaga tgaaataggg gatcagatac tgctctttac
      481 ctgcctgttt tgagtaagta tcgaacagct ctttaaagcg gtcatagctg cccatgcccg
      541 gcttcatcat cttcgataac ggcccttctt cggtatgttc cggggcaatc ttcagataac
      601 cgccgacgtg atgggtcgcc agttctttga tatagcgcgg atcttccacg gcgatgtcat
      661 aacgcacacc agaggcaatc aggatctttt taatgccttt cagatcacgc gcacggcgat
      721 agaggttgat cgttggttcg tggttcgtgt ccatgtgcgg acaaatatcc ggataaacgc
      781 acgacaaacg gcggcaggtt tgctcagcgc gtggcgattt gcagcgcaac atatacatgt
      841 tggcagttgg cccaccgaga tcggaaatta cgcccgtaaa acctggaacg gtgtcgcgga
      901 tcgcttcgat ctcattaatg atcgaatctt cggaacggct ctgaataatg cgcccttcgt
      961 gctcggtgat agaacagaaa gagcagccgc caaagcagcc acgcataatg ttgaccgaaa
     1021 aacggatcat ttcgtaagcc ggaatacgga cattgccata ggccggatgt agcacgcgct
     1081 tgtatggcag cgcaaaaacg ctgtccatct cttcggtaga aagcggaata gcaggcgggt
     1141 tgatccacac atagcggtcg ccgtgttttt gcatcaatgc gcgggcacag cctgggttgg
     1201 tttcgtggtg cagaatacgc gaagcatggg cgtacagcac tttatcgccc ttcactttct
     1261 cgaaagaagg cagcaacacg taggtttttt cccacggttt cgggcgcggt ggctgcacgg
     1321 ttacggcttt ggcttcctgc tttttcggtg ccaccggttt gttatccgcg cacggcaaat
     1381 cttcaccata cggatgcggg attgggtcga tttttccagg ggtatcaaga cgggtggaat
     1441 ccacgccgct ccagccaggc agggcctctt tcacgataat cgcggtatta cgcacatcgc
     1501 ggatttcact aatcggctcg cccatcgcca gacggtgcgc cacctccacc agcggacgct
     1561 caccgttacc aaacatcagc atgtcggctt tcgaatccac cagcacggaa cggcgcacgg
     1621 tatcggacca gtaatcataa tgtgcggtac gacgcagact agcctcaata ccgccgagga
     1681 tcaccggtac atctttccac gcctctttac aacgctgggt ataaaccagt gtggcgcgat
     1741 ccgggcgctt acccgcgacg ttatccggcg tgtaggcatc gtcatgacgt aaacggcgat
     1801 cggcggtata acggttgatc atcgaatcca tgttgccagc agtaacaccg aaaaacagat
     1861 tcggtttacc cagacgcata aagtcgtctt tgctgctcca gtccggctgg gcgatgatcc
     1921 cgacgcgaaa gccctgcgct tccagcatac gaccgcaaat cgccatcccg aagcttgggt
     1981 gatcgacata cgcgtcgcca gtaaccaaaa tgatgtcgca gctatcccag ccaagttgat
     2041 ccatctcttc actagacatc ggcaaaaacg gtgccggtcc aaaacaggcg gcccagtact
     2101 gcggccagga gaacaggtcg cgatccggtt ggatcaggga gatagagctc attttgcttc
     2161 cagaaatgat aaaaaaataa tcaaaggccg gggattataa gccggaacga aagagaaatc
     2221 gaaaagtatt ccatactcgc cctcctcggg cgagtatgaa gattacggta ccggattgac
     2281 taacagttgc ccaatcgagc cacggtccgc catttccagc gtctgactgt tgaagtagaa
     2341 cgggaagtgc gcccaggaag gctgaccgaa ataaacaagc agctccactt gtccgtcaac
     2401 ccaaacggta tctttccagc ctctgtcttc cgggaacggc atcgcaccat tcacgttacg
     2461 gatctggaac atcacgcctt caatatggaa cgcttgcggc tcgtccgcgc gtaccgtcca
     2521 gcgttcccac gttccttgct gcgcggtgac atcaatacgg ttgacgtccc acagttgtcc
     2581 attaataccc gggtcatcac ccagactgat atcgcgactg cgaatcggcg aaccggccat
     2641 gatttcagtt ggcagcaagc gcatcggaag actgtctgtg accagcggca gaaggccggt
     2701 tgggcgcagc gttagcacca gggtagaaac cagaatgctg gatggctcaa agaagccacg
     2761 aatacgatca acaatgctcg ccgcttcgcc acaggtgatc gacacttcat caccgttgct
     2821 catatccacc agaatctcgc ggcgctcgcc cggtgccaac gaaagttgct tcaccgatac
     2881 aggagcaggc aggaatccct gatcgccaga aatcacatgt aacgggcgac tatcgctcat
     2941 ctgtagttga tagcgacgag agttcgacgc gttcagcagt cgcaagcgca cccagccacg
     3001 cgagacttca acgtacgggc tttgtacacc gttaaccagc agcgtatcac caacaaagcc
     3061 gccgcttccc ggttcgttgt attctggcgt accaaagtta tccagccgtt tatcctggat
     3121 aatgaccgga aaatcatcca caccataatg gttggggata ggcagcgact tgctgacttc
     3181 atcttccacc agccacattc cggcaaggcc gttatagacc tgctgagccg tgcggttggg
     3241 agtattggcg tgataccaca gagtagctgc gttctggcga atgggcagta cgggtgccca
     3301 gtcagcgttt ggcgacatca tccgtgccgg accgcccatc agcgggcctg gtacctgtag
     3361 cccggccacc gtcattgaga cattttctgt caggcggttg ctgtaaataa gcttaacatc
     3421 gtcgcccttc cagacgcgga tagtcggccc caggtaacga ccattgattc cccagaccga
     3481 cgcgcgtgtc cctggcgtaa atgaccagtg cgcacgttgt acagtcataa acagcggttg
     3541 cccacggcga gattcaagta gcggcggaac gggtagcggt tgctgttgcc cggctgcgct
     3601 ggccttcagg ggaacagcgc cggcacaaag tgcaatcccc gatgcctgaa tgaactgacg
     3661 ccgactgagt gacatatttg ctccatgtaa aactggctaa agtaataccg tgcaggaaag
     3721 tgttccccgc aaaaatacag attgccgact taaacttttc cggcggcttc gcgttctgcg
     3781 acttctttat cgagctcggc gattttttgt tccattatcg aacgacaatg ggcagccagc
     3841 tcacgaacct gatctttgcc atactgactg acgtcaattg gcggcagcat ttcgacaatc
     3901 accagaccgt tgtgcagtcg attaagatta atcttattcg aagttgtaga gacgcacacg
     3961 ggaataatcg ggacgcccgc cgcaattgcc gcgtgaaatg ctccagtctt gaacggtagc
     4021 aggccgcgac cacggctgcg ggttccttcc gggaacatcc agatggaaat acggcgtttt
     4081 ttgaaatgat tcacgacttc cgcaatggtg ccgtgagctt tagtgcgatt gtttctgtcg
     4141 atcaataagt tgccggttaa ccagtacaac tgcccgaaga aggggatcca cagcaagctc
     4201 tttttaccta ccgtcaccgt cggcggttgc acaatgttcg atgctgtcac catgtcatag
     4261 ttgttctggt ggttagcgat atagatagca ttgccgtagc tttcagcatc tgcaggtttg
     4321 cggcactcaa ctttcaggcc gaacagcggc gcaagacggc caaacatatg cccaaaggtg
     4381 gccacatgtt tcgggttacg cgggctgaaa aggcagtaaa tggagccgaa tacacagact
     4441 aagatgctgt aaatcacggt aataataaga cgaaagatat atagcataac aacctctgaa
     4501 ggtctgagag actggcattg tacgtcacct gaaatcaggt tagggcattg ttaaaagccg
     4561 ctctctggaa aagcgtattg ttaatcgcaa cgcatgaata aacaacggtt tcaagggaat
     4621 ttttatcgtt tccgggggtt gaatacgctg ccggatgacg acttaacgtt tcatccggcg
     4681 ttccttgcaa gcgggaggaa acaggccctc cccggcatat tactcttcgc tatcaccgct
     4741 gctggcacgg cgaggagagt cgatctcaac acgatcgata cgctgcaaac cgcgcatcaa
     4801 cgtaccgcgg cgtccacgtt cgccagtgac tttctgtaac tcttccgggc gcagtttaat
     4861 tttgcgtttc ccaacatgaa tggtcagcgt gctttgcggc ggcagaacgt acaattgcgc
     4921 cagaccatct tctccacgcg cggcttctgc cgatggaatg ttgataatct tgttgccttt
     4981 gcccttcgac agctgcggca gatcgcttac cgggaacatc aacatacggc ctgcctgagt
     5041 gattgccagc agcatatcgg aagcatcttc aatcaccacc ggcggcataa catgggcatt
     5101 ttccggtaag gtgatcaaag ccttacctgc acggttacgc gccaccagat cgttaaaggt
     5161 gcagacgaaa ccgtaacccg catcggaagc catcagcagt ttctgatcgt cgctttccat
     5221 cagcatatgg tcaacggtcg ccccaggcgg caacgttaat ttgccggtga gtggttcgcc
     5281 ctgaccacgc gccgacggca gcgtaatcgg gtcgatggca tagctacgac cggtggaatc
     5341 aacaaacact accggttggt tgctcttacc tttcaccgcc gctttgaagc tatcacccgc
     5401 tttataattc aggcccggcg cgtcgatatc atggccttta gcgctgcgta cccagcccat
     5461 ctgcgacagc acaatggtga caggttcaga cggcagcatg tcgtgctcgc tcatcgcttt
     5521 cgcttcttcg cgttcctgca acggcgaacg acgatcgtca ccgtaggctt gcgcgtctgc
     5581 ctgcagttct ttcttcagca ggttattcat tttacgctcg gaaaccaaaa tgccctgcaa
     5641 ctggtcgcgc tctttttcca gctcactctg ctcaccgcga atcttcatct cttccagttt
     5701 ggcaagatga cgcagtttca gttcgaggat cgcttccgcc tgggtttccg taaggccaaa
     5761 ccgcgacatc agcgccggtt tcggttcatc ttcgttacga atgatctcaa tcacttcgtc
     5821 gatgttgaga aacgccacca gcaaaccttc caggatatgc aggcgcttga ggactttctc
     5881 cagacgatag ttcagtcggc ggcgcacggt atcgcgacgg aacaccagcc attcggagag
     5941 gatttccagc aggtttttca ccgccggacg accatccaga ccgatcatat tgaggttaat
     6001 gcgatagctc ttttccagat cggtggtagc gaagaggtgg ttcatcacct gatccatatc
     6061 cacgcggttg gaacgcggca caatcaccag acgggtcggg ttctcgtggt cagattcatc
     6121 gcgcagatcg tcaaccatcg gcagcttttt gttgcgcatt tgcgcagcaa tttgctccag
     6181 tacgcgcgca cctgaaacct gatgcggcaa tgcgctgata accaccgcgc catcttcttt
     6241 cttccacacc gcgcgcatac gcactgaacc acgtccgttc tcgtagattt tacggatctc
     6301 ggcgcgcgaa gtgataattt ccgcttcagt cggataatcc ggcccctgca cgatatccag
     6361 cagctgatcg agcgtggttt tcggctggtc gattaatgcg attgccgcct gagctacttc
     6421 acgcaggtta tgcggtggaa tatcggtcgc catgccgacg gcaataccgg tggtgccgtt
     6481 aagcaaaatg tttggcagac gggcaggcag cattttcggc tcctgcaaag tgccgtcgaa
     6541 gtttggcacc cagtcagccg tcccctgccc cagctcgctc aatagcagct cggaatattt
     6601 cgacaaccgg gattcggtgt aacgcattgc cgcgaacgat ttcggatcgt ccggcgcgcc
     6661 ccagttcccc tgaccatcaa ccagcggata acggtaagag aacggctgcg ccatcaggac
     6721 catcgcttca taacaggcgc tatcgccgtg cggatggtat ttacccagta cgtcaccgac
     6781 ggtacgggcc gattttttaa atttggcgct ggcattcagg cccagttcag acatcgcata
     6841 cacaatgcgg cgctgaacag gtttaagacc atcgccaata aacggcaacg cacggtccat
     6901 gatcacgtac atggagtagt ttaagtaggc gttttccgta aattcatgta gcgcaaggcg
     6961 ctctgccata tcgctcatta attctgattc ctcaacttat tcgccagcct tcaaaggggc
     7021 aatattgccg gagatactac attatctgcc gcgtcgagtc acaggagatc agcgtgaata
     7081 gcgccgccgg acgtgctccg acggcgataa aggcatcaat gtgccttgat atacaactct
     7141 ttgctgtaga cttgatcggc atagagggca gcacaaggtt gcgcccatgc cacaccactc
     7201 ggcatggggg tccgctccgg gcgattcgag aagttgaggt tatgcgagtc cagggaagcc
     7261 taaagcatca aagtagtcga ctgtaagtat acggttcagc ccagtttagc tgtgtctcca
     7321 ccagtgcccc gcgcctcctg ccataagtgc ggcttgctca tggatacccc cgagacggct
     7381 tacggcgcac cggataataa atttcgcgta tacatggtat tccttaccgg agaaattata
     7441 agcccaacaa caatacgcct gtggcattgt tcgctcaaac ttatcgcgag tgatttggct
     7501 cacactccgg taaagattcc ccatcacgct aacggcacat aaacatccgt ttccagtcgc
     7561 tgttctgtca taccttcggc aagattggtg tagtgaaaca gaatcggtgc tttacgcatt
     7621 ttctcgccgc ttgcaggcag ccagtggcga atgatgcccc ataccgtgtg tgaaatatcg
     7681 tccagctcgc caacgtggcg ggccacggca taacgtccac cggtaagttc accattgctc
     7741 acaccataac gattatcggg aatcggttcg ctaacgctgc cgcagatatc aaagcgaaac
     7801 gctttcggtg cggtggtggc agggtcgtcc caggcgacgc caaaagtctg gctttggtta
     7861 acaggagaaa gccccgtctc tttacgccac atgataaatt tcgccacact gtagttgagc
     7921 aactcagggc tacagcggtg cggcaacatc gccacaggga ttgatgggaa atcgataatg
     7981 tttacatcca gtgtcaggtt tgtcattttg cctccctgcg aaattattcc cgggaacgcg
     8041 tcccgggagc ggtaacaatt acggatttac tttgcggatc tgtttgacgt caatttcaac
     8101 agaattccag tctttatcga cttccccctg aatctcaacc gtatctttcg gcgtcaccgt
     8161 cacaccgttc cagcgtttgt ggtcgatatc aacattgata gtaccgctgg catctttaaa
     8221 cacgtagaga tcgtcagaga tgcgttcaac gatattgccg cgcagggtta cccaggtgtc
     8281 gtcacgccgg gattttgcgc tttctacagt cgttacgctg ccgttcggcc cctggaatcc
     8341 tccggcctga ctttgcgttg ccgatgggcc agaaaaaccg ccctgctctg ctgccatcac
     8401 cggtgcactg cacagggcca ttactgcgat tactgctgcg aattttttca tgtttattac
     8461 tccctttaat gtctgtttcc gagcatttaa caagatagtc cttaacaact tcttaaggga
     8521 aaaaaataaa atttaatgct gtacagagcg cgttacaaca cggtttactg gcagcaaata
     8581 cggttatcgc agggatgaaa aaatgcgaat tttactgata gaagatgaca tgctgattgg
     8641 cgacggcatc aaaactggcc ttagtaaaat gggttttagt gtcgactggt ttacacaagg
     8701 tcgtcaggga aaagaggcgc tttatagcgc accttatgat gcggtgatcc tggatttaac
     8761 cttaccaggc atggatggtc gcgatatttt gcgcgaatgg cgagagaaag gtcagcgtga
     8821 gccggtactg atcctgaccg cgcgtgatgc gttagcggaa cgtgtagaag ggctgcgtct
     8881 gggagctgac gattatctgt gtaaaccttt tgcgttgata gaagtcgccg ccaggctgga
     8941 ggctctgatg cgccgaacca acggccaggc cagcaacgag ctgcgccacg gtaacgtcat
     9001 gctcgacccc ggcaaacgta tcgccacgct ggctagcgaa cccttaacac tgaaaccaaa
     9061 agaatttgcc ctgctggaat tactgatgcg taacgctggt cgggtactgc cgcgcaaact
     9121 gattgaagag aaactgtata cctgggacga agaggtcacc agtaatgccg ttgaagtgca
     9181 tgtgcatcat ctgcgacgca aactcggtag tgattttatt cgtaccgtgc atggtattgg
     9241 ctacacatta ggtgagaaat gaaatttacc caacgtctta gtctgcgcgt caggctgacg
     9301 ctaatctttt taattctggc ctcggtgacc tggctgcttt ccagctttgt cgcctggaaa
     9361 caaacaacgg ataacgtcga tgaattgttc gacacccaac tgatgctgtt tgccaagcgg
     9421 ttaagtacgc tcgatctcaa cgaaatcaac gcggcggatc gcatggcaca gacgccaaat
     9481 aaattaaaac acggtcatgt tgatgacgat gcgctgacct ttgccatctt tacccacgac
     9541 ggcagaatgg tccttaatga tagcgataac ggagaagata ttccctatag ctatcaacgg
     9601 gaaggttttg ctgacgggca actggtcggt gaagacgatc cttggcgttt tgtctggatg
     9661 acctcacctg atggcaaata tcgcatcgtt gttggccagg aatgggaata ccgtgaagac
     9721 atggcgctgg cgattgttgc cgggcaattg atcccgtggc tggtcgcact gccgattatg
     9781 ttaatcatca tgatggtact actgggtcgt gaactcgcgc cgctgaacaa actggcgctg
     9841 gcactacgta tgcgtgaccc tgactcggaa aaaccactaa acgcgactgg cgtacccagc
     9901 gaagtgcgac cactggttga gtcgctaaat caactgttcg cccgcacaca tgcgatgatg
     9961 gttcgagaac gacgctttac ctccgatgca gctcacgaac tacgtagccc gttaacagcg
    10021 ctgaaagtgc aaaccgaagt tgcgcagctc tctgacgatg atccgcaggc acggaaaaaa
    10081 gcactgctcc aattacattc cgggatcgat cgcgctactc gtctggttga tcaactgctc
    10141 acgctatcgc ggctggactc actggataac cttcaggacg tcgcggagat cccgcttgaa
    10201 gatctcctgc aatcgtcggt gatggatatt taccacacgg cgcagcaggc gaaaattgac
    10261 gtgcgactga cactcaatgt ccagggcatc aaacgcaccg gacaaccgct attgctaagt
    10321 ttgttggtgc gaaatttgct ggataacgcc gtgcgttaca gtccacaggg cagcgtggtg
    10381 gacgtcacgc tgaatgccga taatttcatc gtgagggata acggccccgg tgtgacacca
    10441 gaggcactgg cgcgaattgg cgaacgcttc tatcgcccct ccggacaaac cgctaccggc
    10501 agtggacttg ggctatcgat tgtccagcga atcgccaagc tgcatggcat gaatgttgag
    10561 tttgggaatg cggaacaagg tggatttgag gcgaaagtaa gctggtaagg gtaagacttt
    10621 tgctaaattc gcaaaagact ttgcacattt tgctaatttc accgtaccgc tctgtgacgt
    10681 actatagtcg gcaaacgtct caccttgagg ttaaaaaatg agcaacattc tgattatcaa
    10741 cggcgcgaaa aaattcgccc actccaatgg tcaactgaat gacaccctga ccgaagtcgc
    10801 ggatggcaca ctgcgcgacc tcgggcatga tgttcgcatc gttcgcgccg acagcgacta
    10861 cgatgtcaaa gcggaagtac aaaactttct ctgggctgat gtggtgatct ggcagatgcc
    10921 aggctggtgg atgggcgcgc cgtggacagt gaaaaaatac attgatgatg tattcaccga
    10981 aggtcacggg acgctgtatg ccagcgatgg ccgtacccgc aaagatccgt caaaaaaata
    11041 cggttccggc ggcctggtac agggcaaaaa atatatgctt tctctgacct ggaacgcacc
    11101 aatggaagcc ttcaccgaaa aagatcaatt cttccacggc gttggcgtcg acggtgtgta
    11161 tctgccgttc cataaagcaa accaattcct tggcatggaa ccgctgccga catttatcgc
    11221 taatgacgtg ataaaaatgc ctgatgttcc ccgctatact gaagaatatc gcaagcatct
    11281 tgtggaaatt tttggttaac tagagctcag gctttagaag gagttaacca tgcttaccgt
    11341 aatcgcagaa atccgtactc gtcctggtca acatcaccgt caggcggtat tggatcagtt
    11401 tgctaaaatc gttccaaccg tactgaaaga agaaggttgc cacggctatg cgccaatggt
    11461 ggattgcgct gctggcgtga gtttccagtc tatggcaccg gattctatcg tgatgattga
    11521 gcagtgggaa agcatcgcgc accttgaagc gcatctgcaa accccgcaca tgaaggcgta
    11581 cagcgaagcc gtaaaaggtg acgtgctgga gatgaatatt cgtattctgc agccagggat
    11641 ttaatcctgc cttgtttgcc cggccatcct gaccgggcaa tgttctttcc tttaaacctc
    11701 aatctccgcc atgtcgcctt tctcttgcaa ccagttgcgg cgatcttccg agcgtttctt
    11761 cgccagtagc atgtccatca tcgcgtcagt acgctgatcg tcttcatcat cgatagtcaa
    11821 ctgcaccaga cggcgagtgt tcggatcaag cgtggtttcg cgcaattgca tcgggttcat
    11881 ttcccccaga cctttaaaac gctggacgtt cggcttgcct ttcttgcgtt ttaattgctc
    11941 aagtacgccc tctttctctt cttccgtcag cgcgtaataa acctctttcc cgagatcaat
    12001 acggtagagc ggtggcagtg cgacgtaaac gtgaccgtgt ttcaccaacg cgcggaagtg
    12061 ttttacaaac aaagcgcaga gcagcgtggc aatgtgcaga ccatcggagt cagcatccgc
    12121 caggatacag atcttgccgt aacgaagctg gctcaaatcg tcgctgtcag gatcgatacc
    12181 gatcgctacc gaaatatcgt gcacttcctg cgaagccagc acttcgtcgg aagagacttc
    12241 ccaggtgtta aggatcttac ctttcagtgg catgatcgcc tgatattcgc gatcgcgcgc
    12301 ctgcttggca gatccgcctg cggagtcacc ttccacaaga aacagttcgg tgcggttaag
    12361 gtcctgcgcg gtacagtcag ccagtttgcc aggcagcgcc ggaccgctgg tcagcttttt
    12421 acgcaccact tttttagccg cacgcatacg acgctgggcg ctggaaatcg ccatctctgc
    12481 cagcaactcc gccgcctgaa cgttctggtt cagccacagg ataaaggcat ctttcaccac
    12541 gccagaaacg aatgccgcgc actgacgcga agagagacgc tctttggtct gcccggcaaa
    12601 ctgcggatcc tgcattttta ctgacagcac ataggcgcag cgatcccaga tatcttccgc
    12661 cgacagcttt acaccgcgcg gcagaatgtt gcggtattca cagaactcac gcatcgcgtc
    12721 cagcaggccc tgacgcagac cattaacatg ggtaccgccc tgcatcgttg ggatcaggtt
    12781 gacgtagctt tcggtcagca gttcaccgcc ttccggcagc cacagtagcg cccagtccac
    12841 cgcttcagta tcgccagcga aattaccgat aaacggtttt tctggcagcg tcggtaaacc
    12901 gtttaccgct tccgccaggt aatcattcag accgtcctga tagcaccagc gctgttcggt
    12961 gttgttgatc tcatctttaa aggtaatctc aacgccgggg cacagtactg ctttggcttt
    13021 cagcacatgc gtcaggcgtg aaacagaaaa acgcgggcta tcgaagaagg tttcatccgg
    13081 ccagaaatgg acgctggttc cggtattgcg tttaccgcaa gtgccgacaa cctgtaaatc
    13141 ctgcaccttt tcgccatttt caaaggcgat gttataaatc tgaccatcgc ggcgcacgtt
    13201 aacttctacg cgcttcgaca gggcgttaac caccgaaatc cccacgccat gcaggccgcc
    13261 agagaactgg taatttttgt tagagaattt accgcctgca tgcagacggc aaagaatcag
    13321 ttcaaccgcc ggtacaccct cttccgggtg aatatccacc ggcatcccgc gcccatcgtc
    13381 aataacttct aacgactggt cagcatgtaa aataacgtcc acgcgttttg cgtgacccgc
    13441 cagtgcttca tccacactgt tatcaatgac ttcttgcccc aaatggttag ggcgagtggt
    13501 atcggtatac atccccggac ggcggcgaac cggctcaagc ccggtgagta cctcaatggc
    13561 atcagcgtta taagtttgcg tcatggttta agttagtaat tcgagttgat cgtcagagat
    13621 ggtgcagacc aagaaaatcg acgatcgggt tgaaataatc ttcgaagccc gtgaatgcgt
    13681 ggttgccgcc ttctatgaca gtctggcggc aggaagcgta gtacgccacc gcctggcggt
    13741 aatccagcac ttcatctccc gtctgttgca gcagccagat caaatccggc gcttccagcg
    13801 ggtcaatctg catgacttta agatcgtaaa tatggcgtga ctctagcaca tattgctgcc
    13861 cggtgtaggg gttctcgttc tgaccgagat agtccgtcag cagttcaaac gggcgcaccg
    13921 ccgggtttac caccactgcg ggcagcataa aacattgtga caaccaggtg gcgtaatatc
    13981 cccccagtga cgaaccgaca atacccagcg aatcgccgcc atgttccagg acaatggatt
    14041 ccagcagctc tgccgcgtcg gaaggatacg gcggcaactg cggaatgatc atctcaacgt
    14101 cagggtgatg ttccgccagc cagtttttta acaagctcgc ttttgcagag cgcggcgagc
    14161 tgttgaaacc gtgtaaataa agaagcgtag acatcagtag ccttctgaag cggtatcagg
    14221 ttggaaacgt gtgtccgcca ggcgatgcac ctcggtggtc agcgtgccat cagcatgtaa
    14281 ctcgagagta cgccagccgg gcgcgatggt atccagcgta aagttggaac agtgcggctt
    14341 aaactgcaca caggtcgacg gcgttgccag caggcggcga ccgttccagt cgagatccag
    14401 ctcctgatga atatgaccgc acagcaagta tttgacgtgc ggaaacttcg ccagcacggt
    14461 atccagttcg cccgcgttac gcagactgtg ttgatcgagc caactacaac ccgcaggtag
    14521 cggatgatga tgcagcagca gcaacgtatg gcgttctggc gcatcggcca gtttacgttc
    14581 cagccactca agctgaaact cgctcagctc accgtgcggc acgccaaaca cctggctatc
    14641 cagcaacagg atttgccatt gctcaccaat aaacacgcgc ttcgccgggg agatacccgc
    14701 atcctgtaac gcgctgtaca tcgcgggctg gaaatcgtgg ttgcccggca gccagacgca
    14761 gggcgcacga aaacttgcga tgccttcagc gaaatgctga taggccgcag aggattgatc
    14821 ctgcgctaaa tcacctgtcg cgacaatcag gtcgaattcg tgctgatgtg gacgaatcgc
    14881 ctccagcacc gcctggtagc tttcccaggt gtttacccct aacagggctt cgtgcttttg
    14941 tgcaaacagg tgagtatcgg taatttgtaa aatcctgact ctggcctcac cagccagagg
    15001 aagggttaac aggctttcca aatggtgtcc ttaggtttca cgacgctagt aaaccggaat
    15061 cgccatcgct ccatgtgcta aacagtatcg caaccagtcc gctaaaaact ggttaatttg
    15121 atgcttttcg tcgcgttgat gcaacttttt attaggataa tcataccgcg ctttgaagcg
    15181 aaaaatctgc tggcttgaac acacttcagc caccatcgcg tcatgataca gacgcaccgt
    15241 cattgacgga aggctccagt aactgatcgc gggcgcagtc tgttctattg tcaccaaggt
    15301 agtgtatcgg gtcgattcca cagtcgtcag ccgatattgt gcgtttgcca cctgatagct
    15361 tacagtttcg ccgggtgcgt cattgcgcgg taacaaacgg cgcaattgtg aaaagttcat
    15421 ctcgcacagg cgcatcattt caggaaagtc aggtgtgtaa cgcttcattt atgcccactc
    15481 attttttaac gcttggtggt gcagctgcag ccattgcaaa gcgatgaccg acgctgcgtt
    15541 gtcgattttc ccctcttcta cccactggta tgcctgttcc cggcttacca catgaacgcg
    15601 aatatcttcg ttttcatcag ccagaccatg aataccgctt gcggtcgtgg cgtccacttc
    15661 gcccaccata attgacgaac gctcactggt gccccccggg cttgccagga aacttaacac
    15721 cggtttggtc cgtttgacta tcagccccgc ctcttcaatc gcttcgcgac gggcaacatc
    15781 ttccacactt tcaccctctt caatcatccc ggcaaccatc tccagtagcc agggggtttc
    15841 gctagtgtcg tacgcggcaa ttcgaatctg ctcaatcagc acaacttcat cacgcactgg
    15901 gtcaaagggt agcaagactg cggcgtgacc gcgctcaaaa atttcccgcc gcacctcatg
    15961 actcatttgc ccgttgaata gactatgacg aaatctataa agatctaatg aaaaaaagcc
    16021 gcgataaagt gtttctcgtg caataatttc tacatcgttt ttgccaaatg taacgggcag
    16081 gttgtctggc ttaagcattg ttaatgtcct ggcactaata gtgaattaaa tgtgaatttc
    16141 agcgacgttt gactgccgtt tgagcagtca tgtgttaaat tgaggcacat taacgcccta
    16201 tggcacgtaa cgccaacctt ttgcggtagc ggcttctgct agaatccgca ataattttac
    16261 agtttgatcg cgctaaatac tgcttcacca caaggaatgc aaatgaagaa attgctcccc
    16321 attcttatcg gcctgagcct ttctgggttc agttcgttga gccaggccga gaacctgatg
    16381 caagtttatc agcaagcacg ccttagtaac ccggaattgc gtaagtctgc cgccgatcgt
    16441 gatgctgcct ttgaaaaaat taatgaagcg cgcagtccat tactgccaca gctaggttta
    16501 ggtgcagatt acacctatag caacggctac cgcgacgcga acggcatcaa ctcgaacgcg
    16561 accagtgcgt ccctgcagtt aactcaatcc atttttgata tgtcgaaatg gcgtgcgtta
    16621 acgctgcagg aaaaagcagc agggattcag gacgtcacat atcagaccga tcagcaaacc
    16681 ttgatcctca acaccgcgac cgcttatttc aacgtgttga atgctattga cgttctttcc
    16741 tatacacagg cacaaaaaga agcgatctac cgtcaattag atcaaaccac ccaacgtttt
    16801 aacgtgggcc tggtagcgat caccgacgtg cagaacgccc gcgcgcagta cgataccgtg
    16861 ctggcgaacg aagtgaccgc acgtaataac cttgataacg cggtagagca gctgcgccag
    16921 atcaccggta actactatcc ggaactggcg gcgctgaatg tcgaaaactt taaaaccgac
    16981 aaaccacagc cggttaacgc gctgctgaaa gaagccgaaa aacgcaacct gtcgctgtta
    17041 caggcacgct tgagccagga cctggcgcgc gagcaaattc gccaggcgca ggatggtcac
    17101 ttaccgacgc tggatttaac ggcttctacc gggatttctg acacctctta tagcggttcg
    17161 aaaactcgtg gtgccgctgg tacccagtat gacgacagca atatgggcca gaacaaagtg
    17221 ggcctgagct tctcgctgcc gatttatcag ggcggaatgg ttaactcgca ggtgaaacag
    17281 gcccagtaca actttgttgg tgccagcgag caactggaaa gcgcgcatcg tagcatcgtg
    17341 caaaccgtac gttcctcctt caacaacatt aatgcatcta tcagtagcat taacgcctac
    17401 aaacaagccg tagtttccgc tcaaagctca ttagacgcga tggaagcggg ctactcggtc
    17461 ggtacgcgta ccattgttga tgtgttggat gcaaccacca cgctgtacaa cgctaagcaa
    17521 gagctggcaa atgcgcgtta taactacctg attaatcagc tgaatattaa gtcagccctg
    17581 ggtacgttga acgagcagga tctgctggca ctgaacaatg cgctgagcaa accggtttcc
    17641 actaatccgg aaaacgttgc cccgcaaacg ccggaacaga atgctattgc tgatggttat
    17701 gcgcctgata gcccggcacc cgtcgttcag caaacatccg cacgcactac caccagtaac
    17761 ggtcataacc ctttccgtaa ctgatgacga cgacggggct tcggccccgt ctgaacgtaa
    17821 gacaacgtaa agatacgggt tatctgccgc attcttcccc cttctcgctt caatttcgac
    17881 cagccatcct ctattctgat gggtatttac cactggtccc ggaagacaaa aatgaaacgg
    17941 acaaaatcca tacgccacgc atcgttccgc aaaaactgga gcgcacgcca tctgacacca
    18001 gtcgctctcg cggttgccac tgtttttatg ctggctagct gtgaaaagag tgatgaaaca
    18061 gtgtctctct atcaaaatgc tgacgactgt tcagctgcaa acccaggcaa aagcgccgaa
    18121 tgtaccaccg cgtacaacaa tgcgctgaaa gaagccgaac gtactgcgcc gaaatacgcc
    18181 acccgtgaag actgtgttgc tgaatttggt gaaggtcagt gccagcaggc accagcccag
    18241 gctggcatgg caccagaaaa ccaggcgcag gcccagcaat ccagcgggag tttctggatg
    18301 ccgctgatgg ccggttacat gatggggcgt ctgatgggcg gcggcgcggg atttgcacag
    18361 cagccgctgt tctcctcgaa aaacccagcc agcccggctt acggtaaata taccgacgcg
    18421 acgggtaaaa actatggcgc agcccagcca ggccgcacca tgaccgtacc gaagacagcc
    18481 atggcaccaa aaccggcgac cactactacc gttacccgtg gcggttttgg tgaatctgtt
    18541 gccaaacaaa gcactatgca gcgtagcgca accggcacct cttctcgttc aatgggtggc
    18601 tgataccgat ggaaagagtc agtattaccg agcgcccgga ctggcgtgaa aaagcccacg
    18661 aatacggttt caattttcac accatgtacg gcgagccgta ctggtgtgaa gatgcttact
    18721 acaagttgcc cctcgcccag gttgaaaagc tggaagaagt caccgccgaa ctgcaccaga
    18781 tgtgcctgaa agtggtggaa aaagtgatcg ccagcgatga gctgatgacc aaattccgca
    18841 ttccaaaaca cacctggagt tttgtgcgtc agtcatggct gactcaccag ccatcgcttt
    18901 attcgcgtct tgatctggca tgggatggca ctggcgaacc taaactgctg gaaaataacg
    18961 ccgatacacc aacgtcacta tacgaggcgg cgttctttca gtggatctgg ctggaagatc
    19021 agcttaacgc cggtaacttg ccggagggca gcgaccagtt taacagtctg caagaaaagc
    19081 taatcgatcg ctttgtcgag ttgcgtgaac agtatggctt ccagttgctg catctcacct
    19141 gctgtcgcga cacggtggaa gatcgcggaa ccattcagta tttgcaggac tgcgcgacgg
    19201 aagctgaaat cgctaccgag ttcctctaca ttgacgatat cgggttaggt gaaaaaggtc
    19261 agttcacgga tttacaggat caggtaattt ccaacctgtt caagctgtat ccatgggaat
    19321 ttatgttgcg tgagatgttc tccaccaagc tggaggatgc aggcgtacgc tggctggaac
    19381 cggcgtggaa gagcattatc tccaacaaag cgcttctacc gctactgtgg gagatgttcc
    19441 cgaatcaccc gaacctgctg cccgcttatt ttgcggaaga tgatcatccg caaatggaaa
    19501 aatatgtggt taaaccgatc ttctcccgtg aaggcgcaaa cgtgtcgatc attgagaacg
    19561 gcaaaaccat tgaagcagcg gaaggtccgt atggcgaaga agggatgatt gttcagcaat
    19621 tccatccgtt accgaaattc ggcgacagct atatgctgat tggtagctgg ctggtgaacg
    19681 atcaacccgc cggaattggc attcgtgaag accgtgcatt gatcacccag gatatgtctc
    19741 ggttttatcc acatattttt gttgagtaat caatgatgcc ggatggcatt cgccatccgg
    19801 taattgttaa cctatctgca ccgacagcat actcaggctg cccatttcta taccatcgac
    19861 cggaatggta attggctcct gcccatccca cgcacctaag acatacaaca acggcagata
    19921 gtgctccggc gttgggttcg ataacgcgcc accttcatgg tcgaggtaat tcaccagagg
    19981 atgctgctct accgcccctt gccacgtcag attcgctttc acatactcat taaacgacat
    20041 cgcccaagga tatggtgaac tatcaccgtg ccacttcact gtacgcaggt tatgcaccac
    20101 gttgccactg gcgaccaaca ttattccttc atctcgcagc gctgccagtt tgcgccccat
    20161 ttcgaaatgc caggcggcag gtttgctact gtcgatactc aactgcacca tcgggatatc
    20221 ggcgtcagga tacatcttaa tcagcacgcc ccaggaaccg tggtcaaagc ccctggcttc
    20281 tttatccagc gtcaccggga ccggcgctaa cagctcaacc agatgctgtg ccagcacagg
    20341 cgaacccgga gcaggataat gcgtatcgta cagcgcctgc gggaagccac caaagtcatg
    20401 aatcgtgggc ggcgtctcca tcgcggtcac tcctgttcca cgggtaaacc agtgagccga
    20461 aaccaccaca atcgcttgcg ggcgtggcaa tgtcatcccc aacttctgcc agctgcgggt
    20521 atacaaatta tcttccagca cgttcatcgg actaccgtga cctaaaaaca atgctggcat
    20581 acgtgttgaa gacatgatga tatccttaac taagggtgtc attttgatat cctcacaata
    20641 cgcttgttcg gcggagtaag aacccggata acaatgatga tgatcatcag ttattttgac
    20701 gatctgcctg aaggtgaaga tttataagga gttgtcgatg tcagtacctc tcattctgac
    20761 catactggcg ggggcagcca cgtttattgg cgcgtttctc ggcgttctcg gacaaaaacc
    20821 ctcgaaccgc ttactggcgt tttcgctagg ttttgcggcg gggatcatgt tgctcatctc
    20881 attaatggaa atgcttcctg ccgcactagc agctgaagga atatcgcctg tgttgggtta
    20941 tggaatgttt atcttcggtc tgcttggcta ctttggcctg gaccgcatgt tgccgcatgc
    21001 gcatccgcag gatttaatgc aaaaatcggt gcagccgttg ccaaaatcga tcaagcgcac
    21061 agccattctg ctcactctcg gcatcagtct gcataacttc ccggaaggga ttgccacctt
    21121 tgtcacggcg agcagcaacc tggagctggg atttggcatc gcactggccg tcgcgttgca
    21181 caatatccct gaaggtctgg cagtggcagg cccggtttat gcggcaacgg ggtctaaacg
    21241 caccgcgatt ctgtgggcgg ggatttctgg actagcagaa attcttggtg gtgtgctggc
    21301 gtggttaatc ctcggtagca tgatttcccc ggtggtcatg gcggcggttg caggaattat
    21361 ggtggcgctc tcggttgatg aattaatgcc gctcgccaaa gagattgacc ctaataataa
    21421 ccccagctat ggcgtactat gtggaatgtc agtgatggga ttcagtttag tgctgctaca
    21481 aacggcggga attggttaaa aacaacatca gaagttgtcc agcacatgga caacttctgc
    21541 cagcttcacg aaagccttat tctacctttt cggctgtctc ttctctcgta ctgtttaagt
    21601 atttgttccg cgcttcaagc agtgcgtctc tgtcctggcg aaacgcccga caataacgtt
    21661 tcaaatgcag gaaataaccc accacaacca gagcaatcaa tacaatccag taccaggcga
    21721 taaacatcct ttacccttaa tatttaacaa tactaaacat ttagcgcata aatttcacat
    21781 atcctttttc ggatatattc atatggtcgc ataataacgg aacaattata tgattttgat
    21841 cataatgaaa aagaatgcct gatattaatg aaattttctc atagaaagag aagcggaatt
    21901 aacatcttgc atagcagggc aaaataaaaa ccgaagccag ctacaacttc ggtttcttga
    21961 ttaaggcagt aattaagcag cggttttcag ctggctttac gctcatgtgc ctgacggtat
    22021 gccaccaggt cttcaatagt cacgagcgcc atattgtgtt tattggcaaa ctcaatgcac
    22081 tctggtgcac gcgccatcgt gccatcgtca ttagtcagct cacacagcac accagccggt
    22141 ttaaagcctg ccagcgtcat caaatcaata gtcgcttcag tatgaccgcc acgcgtcagt
    22201 acaccacctg cctgcgcgcg aagtgggaaa acgtggcccg ggcgattcag atcagaaggt
    22261 tttgcgccat cggcaatcgc tgcgcgaacg gtcgtaatac gatcagcggc agaaacaccg
    22321 gtagtcacac cttcagccgc ttcaatggtc acggtaaaac cggtgccata ggcgctggtg
    22381 ttattttcta ccatcattgg cagatcaagt tgtttacggc gatcttcagt gatgcacagg
    22441 caaacaatac cgctaccgtg gcgaatggtc agcgccatct gctcaacggt catggtttct
    22501 gccgggaaga tcatatcgcc ttcgttttca cggtcttcat catcaagcac cattacaccg
    22561 cgtccttcac gcagcgcagc cagtgcattt tcaacacgtt cgaaaggcgt accaaaagag
    22621 gaaagtagcg tctgattcat ggtaaaaaaa actcactaaa attatggtta ccagaatcag
    22681 ggcagtctta ggagtggcgg catatagcca aaataacgtg agcgggtcca tgcccgacag
    22741 aatcgttact ctctcccatc cggactctaa ccgtcggccc cggaattaca ccggatctgc
    22801 tgtcctttga gttcgcaccc aaagcgctcg cgggctttca actgagttga tttaccgccg
    22861 gtggggaatt tcgccccgcc ctgagaataa gcggattcac tataacgcta atgattagcg
    22921 gcagcaacgc atagcttcac ataattctgg tttatgactt acccttatcg cactacaatg
    22981 gtactcaaca cctatcagta cagggaaact acaatgattg acccgaaaaa aattgagcaa
    23041 atcgctcgcc aagttcacga atcaatgcct aaaggaatca gggagttcgg ggaagatgtg
    23101 gagaaaaaaa tccgccaaac cctacaagct cagctgacgc gcctcgacct tgtaagccgt
    23161 gaagagttcg acgtccaaac gcaagtcctg ttacggactc gtgagaaact ggcgctgctt
    23221 gaacaaagaa tgagcgaact ggaaaatcgc agtactgaga ttaaaaagca gccagatcca
    23281 gagactctcc ccccaacgct gtaaacattt gtctttaatg cactgtaaat tatgaattaa
    23341 tcttctaatt tacagtgctt tctattacct tatcgcttaa ttccattcag aatatcacca
    23401 caaaaatgaa tgagtgcaat tgaaaatatt attgttatgt atttttttaa gttttactta
    23461 atccatattt agataaacta atttcataaa acccgaaaag taaaatataa tatttgaatg
    23521 attagcaatc cttaaataac agttactaat cattcatttt ctacttatat ttatataaag
    23581 gaatattatg tctgctttta aaaagtcact tcttgttgct ggcgtagcaa tgattctttc
    23641 caataacgta tttgctgacg aaggtcacgg cattgttaaa tttaaaggtg aagttatctc
    23701 tgctccatgt tcaattaaac caggtgacga agacttaact gtaaatctgg gtgaagtagc
    23761 agataccgtt ctgaaaagcg accagaaatc tttggctgag cctttcacta ttcacctgca
    23821 agactgcatg ttgagccagg gtggaaccac ttattcaaaa gcgaaggtta ctttcaccac
    23881 agcgaatact atgacgggtc agactgacct gctgaaaaac accaaagaaa ctgaaatcgg
    23941 tggtgccaca ggcgttggcg tacgtattct gaatagccag agcggcgaag tgactttagg
    24001 tactccggta gtaattacat tcaacaatac taacagctat caggaactga acttcaaagt
    24061 tcgtatggaa tccccgggca aagatgctac cccgggcaac gtttacgctc aggctgacta
    24121 taagattgct tacgagtaat cacattaggc cgggttcgcc cggccacatc attaacggat
    24181 taatgataag tggaacagat gtataaaaaa
//
LOCUS       DAJVUJ010000050        24060 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.050, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000050 DAJVUJ010000000
VERSION     DAJVUJ010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24060
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.050"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            322..549
                     /gene="feoA"
                     /locus_tag="OQQ48_001828"
     CDS             322..549
                     /gene="feoA"
                     /locus_tag="OQQ48_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HCS1952628.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            566..2887
                     /gene="feoB"
                     /locus_tag="OQQ48_001829"
     CDS             566..2887
                     /gene="feoB"
                     /locus_tag="OQQ48_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HCS1952629.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAIDETWQSL
                     KDTFSLSVLMNPIEASKGNGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            2887..3123
                     /gene="feoC"
                     /locus_tag="OQQ48_001830"
     CDS             2887..3123
                     /gene="feoC"
                     /locus_tag="OQQ48_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HCS1952630.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            3326..4204
                     /gene="rpnA"
                     /locus_tag="OQQ48_001831"
     CDS             3326..4204
                     /gene="rpnA"
                     /locus_tag="OQQ48_001831"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HCS1952631.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPDTARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMGCLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLTDI
                     MRFTGVSEEELAAASQ"
     gene            complement(4233..5003)
                     /gene="bioH"
                     /locus_tag="OQQ48_001832"
     CDS             complement(4233..5003)
                     /gene="bioH"
                     /locus_tag="OQQ48_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HCS1952632.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSQGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDDWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWPRSKSYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            5041..5724
                     /gene="gntX"
                     /locus_tag="OQQ48_001833"
     CDS             5041..5724
                     /gene="gntX"
                     /locus_tag="OQQ48_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HCS1952633.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRAARTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCRWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            5783..6358
                     /gene="nfuA"
                     /locus_tag="OQQ48_001834"
     CDS             5783..6358
                     /gene="nfuA"
                     /locus_tag="OQQ48_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HCS1952634.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            6719..8035
                     /gene="gntT"
                     /locus_tag="OQQ48_001835"
     CDS             6719..8035
                     /gene="gntT"
                     /locus_tag="OQQ48_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCS1952635.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(8080..10164)
                     /gene="malQ"
                     /locus_tag="OQQ48_001836"
     CDS             complement(8080..10164)
                     /gene="malQ"
                     /locus_tag="OQQ48_001836"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HCS1952636.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMTAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLETMFADDGVNKLLKD
                     LDRRRRAAAKKQ"
     gene            complement(10174..12567)
                     /gene="malP"
                     /locus_tag="OQQ48_001837"
     CDS             complement(10174..12567)
                     /gene="malP"
                     /locus_tag="OQQ48_001837"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HCS1952637.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQEVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATIGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKLLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLQDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            13179..15884
                     /gene="malT"
                     /locus_tag="OQQ48_001838"
     CDS             13179..15884
                     /gene="malT"
                     /locus_tag="OQQ48_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HCS1952638.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIATVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(15927..16943)
                     /gene="rtcA"
                     /locus_tag="OQQ48_001839"
     CDS             complement(15927..16943)
                     /gene="rtcA"
                     /locus_tag="OQQ48_001839"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HCS1952639.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITSIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGAVRGGEYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIEIDGVTR
                     VSIE"
     gene            complement(16947..18173)
                     /gene="rtcB"
                     /locus_tag="OQQ48_001840"
     CDS             complement(16947..18173)
                     /gene="rtcB"
                     /locus_tag="OQQ48_001840"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HCS1952640.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKDSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAEDLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIKICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQETLETLPSRD
                     LAYFMEGTEYFDDYLKAVAWAQLFASLNRDVMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMTAQSD
                     LVEVIYTLRQVVCVKG"
     gene            18362..19960
                     /gene="rtcR"
                     /locus_tag="OQQ48_001841"
     CDS             18362..19960
                     /gene="rtcR"
                     /locus_tag="OQQ48_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HCS1952641.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWHPTLCLCQQESLVIDRL
                     ELLHDARSCSLFETLKRDIASVSPETEVVSIEIELHNPWDFEEVYACLHDFARGYKFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQTSPPRKKEQPRGAGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFFDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSATGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(19942..20700)
                     /locus_tag="OQQ48_001842"
     CDS             complement(19942..20700)
                     /locus_tag="OQQ48_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HCS1952642.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(20717..21547)
                     /gene="glpG"
                     /locus_tag="OQQ48_001843"
     CDS             complement(20717..21547)
                     /gene="glpG"
                     /locus_tag="OQQ48_001843"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HCS1952643.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVVMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIIAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(21592..21918)
                     /gene="glpE"
                     /locus_tag="OQQ48_001844"
     CDS             complement(21592..21918)
                     /gene="glpE"
                     /locus_tag="OQQ48_001844"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HCS1952644.1"
                     /translation="MDLFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            22108..23613
                     /gene="glpD"
                     /locus_tag="OQQ48_001845"
     CDS             22108..23613
                     /gene="glpD"
                     /locus_tag="OQQ48_001845"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HCS1952645.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAISDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(23665..>24060)
                     /locus_tag="OQQ48_001846"
     CDS             complement(23665..>24060)
                     /locus_tag="OQQ48_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1952646.1"
                     /translation="SVMAGIGWQFSRRTPPELKNRLHCWLVFSPVYFWLILSNFILYI
                     SPEKSALLENIRNFFLTFVWPPLNFSPFWPQPWTDFVGPISAQLGFALGYYCHWCRKN
                     RNQRKKWGDWVTCLSLAILAQGPLFNYLQ"
BASE COUNT         5655 a         6552 c         6080 g         5773 t
ORIGIN      
        1 caaactcata atatattccg gcaatattta tcacttcatt aacaactgaa accttaatta
       61 aacattagcc agtccgggta attcactatt cgaattatat tttcgctgcg atataacctt
      121 gagccacatc aacattgagt cagattatta ttcaaaccaa cattcgcaca cattttaagt
      181 attgctgata gaaaccattc tcattatcat tgtgttattg attatttaat ctctcctttg
      241 ttggcaaatc atctggtctc atgtcgctgt caaacgcccc atgaggtagt tatccagtta
      301 atgagaaaca agtaggcacc tatgcaatac actccagata ctgcgtggaa aatcactggc
      361 ttttcccgtg aaatcagccc ggcatatcgc caaaaactgc tttctcttgg catgttacct
      421 ggctcctctt ttaatgtggt gcgcgtcgct ccactcggcg accccattca tatcgaaacc
      481 cgtcgtgtga gcctggtatt acgcaaaaaa gatctggcct tattagaagt ggaagcggtt
      541 tcctgttaat acggtgataa caacaatgaa aaaattaacc ataggcttaa ttggtaatcc
      601 aaattctggc aagacaacct tatttaacca gctcactggc gcacgtcagc gtgtaggtaa
      661 ctgggctggc gttaccgtcg aacgtaaaga agggcaattc tccaccaccg atcatcaggt
      721 cacgctggtg gacctacccg gcacctattc tctgaccacc atctcatcgc agacctcgct
      781 cgatgagcaa atcgcctgtc actacatttt gagtggcgac gccgacctgc tgattaacgt
      841 ggtggatgcg tctaaccttg agcgtaacct gtacctgacg ctacaactgc tggaactcgg
      901 cattccctgc attgtggcac tgaacatgct cgacattgcc gagaagcaaa atattcgtat
      961 tgaaattgat gctctgtcgg cgcgtctggg ctgtccggtg atcccgctgg tttcaacccg
     1021 tggtcgcggt attgaagcgc tcaagctggc gattgatcgc tataaagcta acgagaatgt
     1081 ggaactggtg cattacgcac agccgctgct caacgaagca gattcactgg caaaagtgat
     1141 gccttccgac atcccgctga aacaacgtcg ctggctgggc ctgcaaatgc tggaaggcga
     1201 tatctacagc cgcgcctacg ccggtgaagc gtcgcagcat ctggatgccg ccctcgcccg
     1261 tctgcgtaat gagatggacg atccggcgct gcacattgcc gatgcgcgtt accagtgcat
     1321 tgctgccatc tgtgatgtgg taagcaacac cctgacggca gaacccagcc gtttcaccac
     1381 tgcggtagat aaaatcgtgc tcaaccgttt cctcggtctg ccgattttcc tctttgtgat
     1441 gtacctgatg ttcctgctgg ctatcaacat cggcggggcg ttacagccgc tgtttgacgt
     1501 cggctccgtg gcgctatttg tgcatggtat tcaatggatt ggctacacgc tccacttccc
     1561 ggactggctg actatcttcc tcgcccaggg cctgggtggc ggcattaaca ccgtgctgcc
     1621 actggtgccg cagattggca tgatgtacct gttcctctcc ttccttgagg actccgggta
     1681 tatggcgcgt gcggcgtttg tgatggaccg tctgatgcag gcgctgggct tgccggggaa
     1741 atcctttgtg ccgctgatcg tcggtttcgg ttgtaacgta ccgtcggtaa tgggtgcacg
     1801 tacgcttgat gcaccgcgtg aacgtctgat gaccatcatg atggcaccgt ttatgtcctg
     1861 cggcgcgcgt ctggctatct tcgcagtatt cgcggctgcc ttcttcgggc agaacggtgc
     1921 gctggcggtc ttctcgctgt atatgctggg tattgtgatg gcggtgctga ctggcctgat
     1981 gctcaagtac accatcatgc gcggtgaagc gacgccgttt gtcatggagt tgccggtcta
     2041 tcatgtacca cacgttaaaa gcctgattat ccagacctgg cagcgtctga aaggcttcgt
     2101 tctgcgtgct ggtaaagtga tcatcatcgt cagcattttc ctgagcgctt tcaacagctt
     2161 ctcgctgagc gggaaaatcg tcgataacat caacgactcg gcgctggcgt ccgtcagccg
     2221 ggtgatcacc ccggtcttca agccaattgg cgtgcatgaa gataactggc aggcaacggt
     2281 tggcctgttt acaggtgcca tggcgaaaga agtggtagtg ggtacgctca acaccctcta
     2341 caccgcagaa aatattcagg acgaagagtt caatccggca gagtttaacc tcggtgaaga
     2401 gctgttcagt gcgatagatg aaacctggca gagcctgaaa gacaccttca gccttagcgt
     2461 actgatgaac cccattgaag ccagcaaagg caacggcgaa atgggtaccg gggcgatggg
     2521 cgtgatggat cagaaattcg gtagcgcagc agcagcttac agctacctga ttttcgtcct
     2581 gctgtatgta ccatgtatct cggtgatggg agccatcgcc cgtgaatcaa gccgtggctg
     2641 gatgggcttc tccatcctgt gggggctgaa tatcgcttac tcgctggcaa cattgttcta
     2701 tcaggtcgcc agctacagtc agcacccaac ttacagcctg gtgtgcattc tggcggttat
     2761 cctgtttaac atcgtggtta tcggtctgct gcgccgcgcg cgtagccggg tggatatcga
     2821 actgctggca acccgcaagt cggtaagcag ttgctgcgca gccagcacca ccggtgattg
     2881 ccattaatgg cttcacttat tcaggtgcgc gatttgctgg cgttacgggg ccgtatggaa
     2941 gcggcccaga taagccagac attgaacact ccacagccaa tgattaacgc catgctgcaa
     3001 caactggaaa gtatgggcaa agccgtgcgg attcaggaag aacctgacgg ctgcctctct
     3061 ggcagttgta aaagctgccc ggaaggaaaa gcctgtctgc gcgagtggtg ggcgcttcgt
     3121 taaccttact ccatcgcctg ttttggaaag cccggtatgc gtctgcatcc gggctttttt
     3181 gcgtgcggct ttccataaaa atgcaactct tgcagcacgg cgtaagttcc tttgaaagca
     3241 tctcgcaggg atgaaaactc gctaatacac aggtgtggag tggcgcgtag agtcgcggca
     3301 ttcaaacaac aggtgaagga acgccatgag caaaaagcag agttccaccc cacacgatgc
     3361 gctgttcaaa ctctttttac gccaaccgga cacggctcgt gattttcttg cgtttcattt
     3421 accggcaccc attcacgcgc tttgtgatat gaaaaccctc aagctggagt cgagcagctt
     3481 tattgatgac gatctgcgtg aaagctattc cgatgtgctg tggtcggtga aaacggaaca
     3541 aggaccagga tacatctatt gtctgattga acatcaaagc acctcaaaca aactgatcgc
     3601 atttcgcatg atgcgttacg ctattgccgc aatgcaaaat caccttgatg ctggatacaa
     3661 aacgttgccg atggtggtgc cattgttgtt ttaccacggt attgaaagcc cctatcccta
     3721 ttcgctgtgt tggctggatt gtttcgccga tcccaaactg gcaaggcagc tttatgcctc
     3781 cgcatttccg ctgattgatg tcaccgtcat gcctgatgat gaaatcatgc agcaccgacg
     3841 tatggcgctg ctggagttaa ttcaaaaaca tattcgtcaa cgcgacctga tggggctagt
     3901 agagcaaatg ggctgcttat taagtagtgg atacgctaat gacagacaaa tcaaagggct
     3961 gtttaattac atactgcaaa ccggcgacgc ggtacgtttt aacgatttta tcgacggcgt
     4021 tgccgaacgt tcaccgaaac acaaggagag tttaatgaca attgcggaaa gattgcggca
     4081 ggagggggaa caatccaaag ccctgcatat agccaaaata atgcttgaat ccggagtccc
     4141 tcttacagac atcatgcgct ttaccggggt gtcagaagaa gagttggctg cggcgagcca
     4201 gtaaatttct gtctcgccat ttcaaaagtt gcctacaccc tctgcttcaa cgccaccagc
     4261 aggtgacaaa actcgaccgg atgcgaaata aacggcgcat gggccgcttt ggcgaagata
     4321 tatgatttgc tgcgaggcca gagtttatcc agcatcggca ccactttgcg cggcaccaga
     4381 ccatcgagat agccatacaa tcgcaaaaac ggcatgggca cgttttgcag cggctgacgg
     4441 agatcaaccg ttttcaggat ttccagcccg ccattaagca cgtcaacctc cggcatcggt
     4501 aacgccagaa cggttttctt caacgcccgc gcatcctggc gcgccgtttc agtccccatg
     4561 gtttgtaacg ccaggaaccg ctccactgta cgctgaaaat catcactgag ttgctgctga
     4621 aatcccgcca gcacgtccgg ttttatcccc ggccaatcgt cacgagcact aaaacaaggt
     4681 gacgacgcca cggtgaccag cgcctgaaca cgctcgggat gggttaacgc aatctggctt
     4741 gccaccagcc cgcccagact ccagcctaac caaatggctt tatcaggtgc ctgttgcagc
     4801 acggcttcgg ccatatcagc aagtgacagc gcaccaaatc cctggctgcg cccgaagccg
     4861 ggcagatcaa caaggtgcag cgtaaaatgc gagctaagtt cctcgtcaat gcaacgccac
     4921 acttcggcat tcagtcccca tccgtgcagc agcacaagat gaacattccc ctgacctttg
     4981 gtctgccacc agatgttatt catccgctat tgttctcttt tgacttacaa ggatgaacat
     5041 atgctaacag taccgggatt atgctggcta tgccgaatgc cgctggcgtt aggtcattgg
     5101 gggatttgtt cggtctgctc acgcgccgcc cgtacagata aaacgttatg cccacaatgt
     5161 ggattacccg ccacacactc acatcttccc tgcggtcgct gcctgcaaaa accaccgccc
     5221 tggcaaagac tggtcacggt tgccgactat gcgccgccgt taagtccgct tatccaccag
     5281 cttaaatttt cccggcgcag tgaaatcgcc agcgccctgt cacgtctgtt actattggaa
     5341 gtcttacacg ctcgtcgcac caccgggttg caattgccgg atcgcatcat cagcgttccg
     5401 ttatggcagc ggcgtcactg gcgtcgggga tttaatcaga gcgatttgct gtgtcagccg
     5461 ttatcacgct ggttgcactg ccgatgggat agcgaagccg tcacacgaac acgggccact
     5521 gcgacccagc attttctcag tgcccggcta cgcaagcgca acctgaaaaa tgcctttcgt
     5581 cttgaattgc ccgtgcaagg tcgccatatg gtgattgtgg atgatgtcgt taccaccgga
     5641 agtaccgtcg cagagattgc gcagttgctt ttacgcaatg gtgcggcgac tgtccaggtc
     5701 tggtgccttt gtcgaacctt gtagagcctc gatgataggc gtattataac caactaaaat
     5761 agtcaactat taggccatta ctatgatccg tatttccgat gctgcacaag cgcactttgc
     5821 caaactgctg gcaaaccagg aagaagggac acaaatccgc gtatttgtga ttaaccctgg
     5881 cacgcctaac gctgaatgtg gtgtttctta ttgtccgccg gatgccgtgg aagccaccga
     5941 cactgccctg aaatttgacc tgctgaccgc gtatgttgat gagttaagcg ctccttacct
     6001 ggaagatgca gagatcgatt ttgttaccga ccagttgggt tctcagctaa cactgaaagc
     6061 gcctaacgcc aaaatgcgta aagtggcaga cgatgcacca ctgatggagc gcgtggagta
     6121 tatgctgcag tcgcagatca acccacagct tgctggtcac ggtggccgcg tttcactgat
     6181 ggaaatcacc gaagacggtt acgccattct gcaatttggc ggcggctgta acggttgttc
     6241 catggtcgat gtgacgctga aagaagggat cgagaagcag ctgctaaacg aattcccgga
     6301 gctgaaaggt gtgcgcgatc tcaccgaaca ccagcgcggc gaacactcct actactaagt
     6361 tatcccctct tttgtagatt gcccgatgcg acgctaaagc gtcatttcgg gcctacgttg
     6421 aacatgtgcc agacgttggc gcagcattgc tttggcaaat ccctcccttc gtttctccgc
     6481 gataatatga ccaacctctc ataatttaaa tttactccgc tctggtgatt ctcaaacgcc
     6541 agatgttacc ggtatcattc acatgggtac caaacatact cctgacatct gactacaata
     6601 attagtttta gtgggtatca gtcgtggtgc cgcaatatct ctgttcccga ttgggataat
     6661 tagagtttgt cgtcagaaaa ttgacgttac ccataacaaa tgaaaggcca ggtaaatcat
     6721 gccattagtc attgttgcta tcggtgtaat cttgttgttg ctcctgatga tccgtttcaa
     6781 aatgaacggc ttcatcgctc tcgtcctcgt ggcgcttgct gttggattaa tgcaagggat
     6841 gccgctggat aaagttattg gctccatcaa agccggtgtc ggcgggacgc tcggtagcct
     6901 tgccctgatc atgggttttg gcgcgatgct gggcaaaatg ctggcagact gcggtggcgc
     6961 acaacgtatc gccaccaccc tgattgccaa atttggtaaa aaacacatcc agtgggcggt
     7021 ggtactgacc ggttttaccg ttggttttgc cctgttctat gaagtgggct ttgtgctgat
     7081 gctgccgctg gtgtttacca tcgcggcttc tgcgaatatt ccactgctgt atgttggtgt
     7141 accaatggca gctgcactgt ctgtgaccca cggcttcctg ccaccgcatc cgggtccgac
     7201 tgcgattgcc accattttca atgccgacat gggtaaaacc ctgctgtacg gtactattct
     7261 ggcaatcccg accgtgattc tcgccggtcc ggtttacgct cgcgtgttga aaggtatcga
     7321 taagccgatc cctgaaggtc tgtacagcgc gaaaaccttc agcgaagaag agatgccgag
     7381 ctttggcgtc agcgtctgga cctctctggt gccggtagtg ctgatggcga tgcgtgcgat
     7441 tgccgaaatg atcctgccga aaggtcacgc tttcctgccg gtagcggagt tcctcggtga
     7501 cccggtaatg gctacgctga ttgccgtgct gattgcgatg ttcacctttg gtctgaaccg
     7561 tggtcgttca atggatcaga ttaacgacac gctggtttct tccatcaaaa tcattgcgat
     7621 gatgctgttg atcatcggtg gtggcggtgc gttcaagcag gtgctggtag acagcggcgt
     7681 ggacaaatac attgcttcca tgatgcacga aaccaacatt tctccgctgc tgatggcctg
     7741 gtcgattgct gccgtactgc gtatcgcgct gggttccgca accgttgcgg caatcactgc
     7801 gggtggtatc gcggcaccgc tgattgcaac gacgggtgtt agcccggagc tgatggttat
     7861 tgcggtgggt tccggtagtg tgattttctc tcatgtgaac gatccgggct tctggctgtt
     7921 caaagagtac tttaacctga ctatcggcga gaccatcaaa tcctggtcga tgctggaaac
     7981 cattatctcg gtgtgcggtc tggtaggctg tctgctgctg aatatggtga tttgataaca
     8041 ctgactgccg gatgcatcgc atccggcatc aaccgcactc tactgcttct tcgctgcagc
     8101 tctgcgccgt ctgtccaaat ccttcagcaa cttattcacg ccatcatcgg caaacatcgt
     8161 ctcaagcgtt gcggaaagct tgcgtcgcca gtttttatac tggtaactgg tgccaggaat
     8221 attcaccggt tcggccatat ccagccagtc ttccggctgt agtcctaaca gagcactgtt
     8281 actgtcggca atgtagcgct gcaaaccacg attaagcgtc ggcgtcatcg acatcaacga
     8341 tgccttatgc ccggcacgtt ttggcagaca accatattta tgcagtgcat ccagcagccc
     8401 ttgcttcgcc agttcgcgat cctgatacag accgcgcagt accacttcat ccggatacag
     8461 ccccagggtt ttgcccaacg ttagatcccc gctctcccag taaccgcgca gcgtaggcag
     8521 gtcatgtgtc gccgcaaccg ccatcgactg ctccggatac gctttcggtg cacggaacgt
     8581 cttctcgtgg tcgttttcga aatagagcac tttgtaggag tacacaccgc tgctgcgcag
     8641 cttaccgaca atctctaccg gtacggtacc gagatcttca ccaatcacca tacagcgatg
     8701 acgtttactt tcgagtgcca gaatcgagag cagatcatcc accggatagt gaacatacgc
     8761 gccctgatct gccgtctcgc catacggtat ccaccacaaa cgcagcatcg acatcacatg
     8821 gtcaattcgt aatgcgccgc agttttgcat attggcacgc aacagctcga taaacggttc
     8881 ataggcacgc gcggtgatga tatgcgggtc cattggcggt aatccccagt tctgccccaa
     8941 cgggccgagg atatccggcg gcgcgccaac cgacgctttc aggcaatata gttcacggtc
     9001 acaccaggtt tccgccccac cttccgctac gccaaccgcc agatcacgat acaagccaat
     9061 cggcatttca tagccctggc ttatctccca gcaggcggca aactggctgt aagccagcca
     9121 ctgcaaccag agataaaaat cgacgtcatc acgatgttct tcgcagaact gacgcacttc
     9181 tggtgaatcc acgttctgat acatctctgg ccatgcaggc cagccccagc gcatttcgtc
     9241 ctctttcact tgctgggcat gtagcgcatc aaaggctgcc tgccagaaca ggctgtcgcc
     9301 ctgctctgca acaaactggc gaaacgcggt catctgctca tcatcacgtt gcgcgaaacc
     9361 tttccacgcc attcgtaatg ctgtcatttt tagggcggta accgtggagt aatcgaccca
     9421 gtcggcatcg cgcgcctgtt gcagcgtctg ttgcgtggtc ggcaactgcc accaggcctg
     9481 agcctcttcg ctaagatgga aatcttcaac ggcgttaacg tcgatataaa tcacattcag
     9541 ccaacggcga gaagacggac tgtatgggct ggcactctcc ggatttgccg gatagagcgc
     9601 atgaatcggg ttcaggccga tgaacgaccc gccacgtttt gccacatcca ccagcatcgc
     9661 tttgagatcg ccaaaatccc caatacccca gtttttttcc gatcgcagcg tataaagctg
     9721 aacgcaggca ccccacagct tttgtttatt cagcaacgcc tgcggttcgt aacagcgttt
     9781 cggggcgaca atcacccggc aatgcgcgcg ctggtcgtcc tgggtgagtg tcagcgtgtg
     9841 ataaccttcc ggcagcttcg tcggtagatt gaacgctttg ccccccgtta catggccttt
     9901 gtactgtgtt ccttcttcgg tggtcagcag ccagctatat tcgccgctgc cctccaccac
     9961 catcggcatt tttttgccgc tggtataaac catgacattc gggactggcg ttaccgccac
    10021 tttcgtggcg gtacgttgat gcatcgcgtc aagcaaacgc cgtttggttt cggcgctaat
    10081 cgactgcggt ttaccgtggg cattgatgta attggggcta atccccgccg ccagcgcggc
    10141 attatccaga cgtttgcttt ccatcgagct tccttagcgt tttgcctgcc agatacgagc
    10201 ctgataatcg cgaatagagc gatccgagct aaacataccg cagcgggcgg tattgaggat
    10261 cgccgcgcga gtccaggcct cttggtcgcg gtacagcaca tccacctgct tttgtgcctc
    10321 aacataggct gcgaaatccg ccatcaccag atacggatcg ccgccttgtt tgccgatact
    10381 gtgcagcatc tggtcgaagg catgcttatc gccgtcgctg tatttaccgc tttccagctc
    10441 tttcaatact gcgtccagca ccttatcttt cttccgccat ttcaccgggt cgtagccttt
    10501 ggccagaatt gccttcactt gttccacggt atgaccaaaa ataaagatat tttcttcacc
    10561 gactttctcg gcgatttcaa cgttcgcacc atccagcgta ccgacagtaa gcgcaccatt
    10621 gagcgccagt ttcatattgc cggtaccgga agcttcttta cctgcagtcg aaatttgttc
    10681 ggagatatcc gccgccggga tcagtttttc cgccgccgaa acgcaataat cctgcaggaa
    10741 caccaccttc aacttatcgc caaccagtgg atcgttgttg atcacgtcag ccactttgtt
    10801 gatcgcaaag ataatattct tcgccaggta gtagcccggt gccgctttcg cgccgaagag
    10861 gaagacgcgc ggtacgcgat cagcctgcgg gttttcacga atttctttgt acaacgccag
    10921 aatatgcagc agattcaggt gctggcgttt gtactcatgc agacgtttaa tctgaatatc
    10981 gaaaatcgcc tgtggattga tctcaatacc ggtacgaact ttcacaaact ccgccagacg
    11041 gactttattc gcctgcttga tctcgcgata ttgctgacgg aatttcgcat catcagcgaa
    11101 tttttccaga ttgatcagct gatcgagatc gttagcccac tctttttgca gtaatttatc
    11161 caacagagcc gccagtgcag ggttgcactg tttgatccag cgacgtgggg taataccgtt
    11221 ggtgacgtta tggaatttgt tcggccatag ctggtgatat tccgggaaca gatctttcac
    11281 caccagatcc gagtgcagcg ccgcaacacc gttcaccgcg aaaccgccaa ccacacacag
    11341 gttcgccata tgcacttgtt tatcgtgcac caccgccagt ttggcccaca ctttttcatc
    11401 gcccggccag gttttctcca ccagcgtttt aaagcgagta ttaatttcgt taataatctg
    11461 catatggcgc ggcagtaagc ctttcaccag tttcacatcc cagcgttcca gcgcttctgg
    11521 catcagggta tggttggtgt aagcgaaagt tttgctggta atggcccagg cgtcatccca
    11581 gctcatctgg tgctcatcga tcagcacgcg cagcagttct ggaatcgcga tagttgggtg
    11641 ggtatcgttc agctgaataa cttcataatc cgccagttcg tgcagtttac gccccgccag
    11701 atgatggcga cgcaaaatat ccgctaccga acaggcacac tggaagtact gctgcatcaa
    11761 gcgcagcttt ttaccggcag tatggttgtc gtttggatag agaactttgg tcagtttttc
    11821 cgcattgatg ccctgctgtt cggcacgcag gaaatcaccg tcgttaaatt tagtcagatc
    11881 aaacggatgc gcgtgcgtcg cctgccacag acgcagcggc tgcgccacgc cattacgata
    11941 gccgacaacg gggagatccc acgcttgacc ggtaatggta aactccggct cccagcgtcc
    12001 gtctttcgtc actttaccgc caatccctac ctgcacatcc agtgcttcgt tgtggcggaa
    12061 ccacgggtag ttactgcgat gccagtcatc cggcgcttca acctgtttgc catcgacaaa
    12121 agactggcgg aacaaaccat attgatagtt cagaccgtaa cccgtcgcag actgaccgat
    12181 agttgccatt gagtcgagga agcacgccgc cagacgtccc agaccaccgt tacccagcgc
    12241 cgggtcgatc tcttcttcca gcaggtccgt cagattgatg tcataagcct tcaacgaatc
    12301 ctgtacttcc tgataccagc cgagattcaa caggttgttg cccgtcaggc gaccaatcaa
    12361 aaactccatt gagatgtagt taacatgtcg ctgattcgcc accggcttgg cgaatggctg
    12421 agcacgcagc atttcggcca gtgcttcact cactgctagc caccactggc gaggagtcat
    12481 ttcagccgca gaatttaagc cataacgctg ccactgacgt gaaagcgctt cctgaaattg
    12541 cttatcgtta aaaataggtt gtgacatagg agttccactt ttcttagatt ttcaacacaa
    12601 cgttatcgct agtttgccag gctcgatgtt gaccttcctc atcctgcggg ggattaggca
    12661 gggaggagtt gcggggatga gcaaggaaat gtgatctcaa ccacttaaag ctagtgcaaa
    12721 ccacaggatt agcagcaaat caatgcaata cagcgcagaa aatctgtatc taagtgcaaa
    12781 aaatggccgt tgcgtatttt caaaaagcgg aaggtaactc tataaattaa gtaaaggagt
    12841 gaaacagttt cacaagtaaa atatccagtg tgctccatct cattcttaat agatttatta
    12901 agatcatctt tttagatggc actttcatca ggaatgaaga agaaaccctt gcttaaatga
    12961 atctgatgaa cataagggaa accagtattc acgctggatc agcgtcgttt taggtgagtt
    13021 gttaataaag atttggaatt gtgacacagt gcaaattcag acacataaaa aaacgtcatc
    13081 gcttgcatta gaaaggtttc tggccgacct tataaccatt aattacgaag cgcaaaaaaa
    13141 ataatatttc ctcattttcc acagtgaagt gattaactat gctgattccg tcaaaactaa
    13201 gtcgtccggt tcgactcgac cataccgtgg ttcgtgagcg cctgctggct aaactttccg
    13261 gcgcgaacaa cttccggctg gcgctgatca cgagtcctgc gggctacgga aagaccaccc
    13321 tcatttccca gtgggcggca ggcaaaaacg atatcggctg gtactcgctg gatgaaggtg
    13381 ataaccagca agagcgtttc gccagctatc tcattgccac cgtgcagcag gcaaccaacg
    13441 gtcactgtgc gatatgtgag acgatggcgc aaaaacggca atatgccagc ctgacgtcac
    13501 tcttcgccca gcttttcatt gagctggcgg aatggcatag cccactttat ctggtcatcg
    13561 atgactatca tctgatcact aatccagtga tccacgagtc aatgcgcttc tttattcgcc
    13621 atcaaccaga aaatctcacc ctggtggtgt tgtcacgcaa ccttccgcaa ctgggcattg
    13681 ccaatctgcg tgttcgtgac caactgctgg aaattggcag tcagcaactg gcatttaccc
    13741 atcaggaagc gaagcagttt tttgattgcc gtctgtcatc gccgattgaa gccgcagaaa
    13801 gcagtcggat ttgcgatgac gtttccggtt gggcgacggc actacagcta atcgccctct
    13861 ccgcccggca gaatacccac tcagcccata agtcggcacg ccgcctggcg ggaatcaatg
    13921 ccagccatct ttcggattat ctggtcgatg aggttttgga taacgtcgat ctcgcaacgc
    13981 gccattttct gttgaaaagc gccattttgc gctcaatgaa cgatgcgctc atcacccgtg
    14041 tgaccggcga agaaaacggg caaatgcgtc tcgaagagat tgagcgtcag gggctgtttt
    14101 tacagcggat ggatgatacc ggcgagtggt tctgctatca cccgctgttt ggcaacttcc
    14161 tgcgccagcg ctgccagtgg gaactggcgg cggagctgcc ggaaatccac cgtgccgccg
    14221 cagaaagctg gatggcccag ggatttccca gcgaagcgat tcatcatgcg ctggcggcag
    14281 gcgatgcgct gatgctgcgc gatattctgc ttaatcacgc ctggagtttg ttcaaccata
    14341 gcgaactgtc gctgctggaa gagtcgctta aggccctgcc gtgggacagt ttgctggaaa
    14401 atccgcagtt agtgttattg caggcgtggc taatgcaaag ccaacatcgc tatggcgaag
    14461 ttaacaccct gctagcccgt gctgaacatg aaatcaagga catcagagaa ggcaccatgc
    14521 acgcagaatt taacgctctg cgcgcccagg tggcgattaa cgatggtaat ccggatgaag
    14581 cggaacggct ggcaaaactg gcactggaag agctgccacc gggctggttc tatagccgca
    14641 ttgtggcaac ctcggtgctg ggtgaagtgc tgcactgcaa aggcgaattg acccgctcac
    14701 tggcgctaat gcagcaaacc gaacagatgg cacgccagca cgacgtctgg cactacgcct
    14761 tatggagttt aatccagcaa agtgaaattc tgtttgccca agggttcctg caaaccgcgt
    14821 gggaaacaca ggaaaaagcg ttccagctga tcaacgagca gcatctggaa cagctgccga
    14881 tgcatgagtt tctggtgcgc atccgagcac aactgttatg ggcctgggca cggctggatg
    14941 aagccgaagc gtcggcgcgt agcgggattg aagtcttgtc gtcttatcag ccacagcaac
    15001 agcttcagtg cctggcaatg ttgattcaat gctcgctggc ccgtggtgat ttagataacg
    15061 cccgtagcca gctgaaccgt ctggaaaacc tgctggggaa tggcaaatat cacagcgact
    15121 ggatctctaa cgccaacaaa gtccgggtga tttactggca aatgaccggc gataaagccg
    15181 ccgctgccaa ctggttgcgt catacggcta aaccggagtt tgcgaacaac cacttcctgc
    15241 aaggtcaatg gcgcaacatt gcccgcgcac aaatcttgct gggcgagttt gaacctgcgg
    15301 aaattgttct cgaagaactc aatgaaaatg cccgcagtct gcggttgatg agcgatctca
    15361 accgtaacct gttgctgctt aatcaactgt actggcaggc cggacgtaaa agtgacgccc
    15421 agcgcgtgtt gctggacgca ttaaaactgg cgaatcgcac cggatttatc agtcattttg
    15481 tcatcgaagg cgaagcgatg gcgcaacaac tgcgccagct gattcaactc aatacgctgc
    15541 cggaactgga acagcatcgc gcgcagcgta ttctgcgaga aatcaatcaa catcatcggc
    15601 ataaattcgc ccatttcgat gagaatttcg ttgaacgtct gctaaatcat ccggaagtgc
    15661 cagaactgat ccgcaccagc ccgttgaccc aacgtgaatg gcaggtcctg gggctgatct
    15721 actccggtta cagcaatgag caaattgcgg gcgagctgga agtggcggca accaccatca
    15781 agacgcatat ccgcaatctg taccaaaaac tcggcgtcgc ccaccgtcag gccgcggtac
    15841 aacacgcgca gaaactgctg aagatgatgg gatatggggt gtgagtttag ccggataatg
    15901 cattggatcc ggtttacatc tttacatcat tcaatactca cccgcgttac gccatctatt
    15961 tctatcaaac caaaccgcac cggcaagaaa cgctccacca cggcgatatt ggtcagcaaa
    16021 tggcatgaag gatgggcgac cgtaaattct cccgcgcccg ccagcgccat cggtagcacc
    16081 agctggtcgg cgagatattc ccccactgcc gccgggcttg ccaggtagcg tttcacctct
    16141 ttcactaact gcgcggctac cacctcggca ctgacgcgct tttcaccgac gacgaaaaag
    16201 cgttcagtga tattttcact ttcgacttcc agcgagacgg tattacccgg cccctggtcg
    16261 cgcggcaggt tatgaatatt ctgttcatgc agggaaaaac tccccgccag tgtagcgatt
    16321 tcacgctcag caacatggcg cggcacacca gctaatagaa cttctccacg catctgcaca
    16381 atgttcccgc gctcgccaag ttgcaaggtg ttaaacgatg ccacgggcga gacttccgtt
    16441 gccaccacgc cgcctccggc aggataaaaa ccgtgacgta acagcgtggt ttgctgatga
    16501 attcctattt tcgccagcag cggctccagc acccggcgga taaaatccgc aggcggcgcc
    16561 gacgggttat cggtgcctcc gctcacttca acacgcgaag gtccatcggc aaaccacagc
    16621 gcgggcagca ccgtttgcag caccagcgta cagcttccgg cactaccgat agcaaagcgg
    16681 tactcgccgc cgcgcacggc gccaggccgg aagaccagac gctgcgaccc cagctccgcg
    16741 ccttccaccg ttgccctaca aatttccgca gccgctttta ccgcggtcag atgctggcgc
    16801 aacagccccg gtttcgcccg cccggcacga atgctggtga tggtaaatgg ctggccggtt
    16861 atcatcgaca ggctcagcgc cgagcgcaga atctgcccgc caccttcgcc ctgtgcgcca
    16921 tccagcgcaa tcatcctttt catcatttat ccttttacgc acaccacctg acgcagggta
    16981 tagataactt ccaccagatc gctttgtgcc gtcatcaccg catcaatatc tttatacgcc
    17041 atcgggattt cgtcgatcac ttcggcatct ttacggcatt ccacatgcgc ggtggcacga
    17101 atttgatctt ccacgctgaa cagttttttc gctttagttc ggctcattac ccgcccggca
    17161 ccgtggctgc acgaacagaa cgactcttca tttcccagcc cacggacgat aaagcttttt
    17221 gctcccatcg aaccgggaat aattccatat tgaccagcac gcgcagacac cgcgccttta
    17281 cgcgtcacgt agatctcttc accaaagtgc tgttcttttt gcacatagtt gtggtgacag
    17341 ttgatctctt ccatcgccag cgtttgtggc tgtctgaccg ttttctgcgt aatgctctgc
    17401 aatgccgtta ccacgttttc catcatcaca tcgcggttaa ggctggcaaa aagctgcgcc
    17461 caggccacgg ctttcaggta atcatcaaag tattccgtac cttccataaa gtacgccaga
    17521 tcacgcgacg gcaacgtctc aagcgtttcc tgcatctctt tttgtgccag atcgataaag
    17581 taagtcccga tggcgttacc aattccgcgt gaaccggagt gcagcataat ccacacctgg
    17641 tccgactcat caaggcagat tttaataaag tggttaccgg ttcccagcgt tcccaggtgt
    17701 ttatagttat tggtattcag gaaacgggga tatttttgcg ttaaccactg ataaccggct
    17761 tcaagctcag cccatttcgc atcgacgttg acaggcgggt tttcccatgc gcctttatca
    17821 cgtttacaac gcccggtagt acgcccgtgc ggcacggccg tttcaatcgc ctgacgcagc
    17881 tctgccaggt tttcaggcag gtcttccgcc gttaacgcgg tacgcagcgc gttcattcca
    17941 cagccaatat ccacgcccac cgccgccgga ataatcgccc ctttggtcgg gatcacgcta
    18001 ccaatggtgg aatctttacc caggtgtaca tcaggcatta ccgcaatatg tttgaaaata
    18061 aacggcatct tcgccgtatt aataagttgc tgacgcgcat cggcttctac cggcacgcct
    18121 ttggtccaca tttttaccgg ggcattttca gtggtcagta attcgtaatt cattttgttt
    18181 ttctcttttc gttgtttgct gtcctgataa ttgcaaccgt cgtgccagaa aattaaataa
    18241 gtaaattaat tttttaattc attgttttta aaaagattat atctttatgt ccgtaaccgg
    18301 agatttcccg caaagccgat ttaccgataa tgaaatatcg tcttttataa ggatatctaa
    18361 gatgcgtaaa acagtagctt ttggctttgt cggaacggtg ctggattatg ccgggcgcgg
    18421 cagtcagcgc tggtcaaaat ggcatccgac actctgttta tgccagcaag aatcgttggt
    18481 catcgatcga ctggaattat tgcatgacgc ccgctcgtgc tcgctatttg aaacgcttaa
    18541 acgcgatatc gccagcgttt cgccagaaac agaagtggtg agcattgaga ttgaactgca
    18601 taacccgtgg gatttcgaag aggtttacgc ctgcctgcat gatttcgccc gtggttacaa
    18661 gtttcagcca gaaaaagaag actatttaat tcacatcacc accggcaccc acgtcgcgca
    18721 gatttgctgg tttctgctgg cggaagcgcg ttatctaccc gcccggttga tacaaacttc
    18781 accaccgcgc aaaaaagaac aaccgcgcgg tgccggtgaa gtgacgatta tcgatctcga
    18841 tctaagccgt tataacgcca tcgccagccg ctttgccgag gaacgccagc aaacgcttga
    18901 ttttcttaag tccggcattg ccacgcgtaa cccccacttc aaccgcatga ttgagcagat
    18961 tgaaaaagtg gcgattaaat cccgcgcacc gatcctgctt aacggcccaa ccggtgcggg
    19021 aaagtcattt ctggcgcgac gtatatttga attaaaacag gcgcggcatc agtttagcgg
    19081 cgcgtttgtg gaagtgaact gcgccaccct gcgcggcgat accgccatgt cgacgctgtt
    19141 tggtcatgtg aaaggcgcgt ttaccggggc gcgggaatct cgtgaaggtt tattacgcag
    19201 cgccaacggc ggaatgttgt tttttgatga gattggcgaa ctgggcgcag acgaacaggc
    19261 aatgctgcta aaagccattg aagagaaaac cttttacccg tttggcagcg atcgccaggt
    19321 gagcagtgat tttcagctta tcgccggaac ggtgcgcgat ttgcgccagc tggttgccga
    19381 aggcaaattt cgcgaagatc tgtacgcgcg gatcaatctc tggaccttca ccctgccggg
    19441 tctacgccag cgccaggaag atattgaacc gaaccttgat tatgaagtgg agcgccacgc
    19501 cacactcact ggcgacagcg tgcgttttaa caccgaagcg cggcgcgcct ggttggcttt
    19561 tgcgacctct ccccaggcaa catggcgcgg taactttcgc gaactttctg ccagcgtcac
    19621 gcggatggcc acctttgcca ctagcggacg catcactctg gacgtggttg aagatgagat
    19681 aaaccgtctg cgctataact ggcaggagag ccgcccctcc gcgcttacgg cgttgctggg
    19741 cgcagaggca gaaaacatcg atctcttcga ccgtatgcaa ctggaacacg ttatcgctat
    19801 ctgccgccag gcaaagtcgc tttctgcaac cggacgacag ctttttgatg tttcgcgcca
    19861 gggaaaagcc agcgtcaatg acgcggatcg gctacgcaaa tacctggcgc gttttggtct
    19921 gacgtgggaa gccgtgcagg atcagcacag ctccagttga atatgatggt ccgtcagcac
    19981 ctgcattacg cttactggcg gcggggcgtc ggtgtagacg gcatctacca tgctgatgct
    20041 gcccatattg accatcgcgt tacggccaaa tttcgagtga tcgacaacca gcataacgtg
    20101 gcgcgagttc tcaataatgg cgcgcttggt gcgaacttcg tggtaatcga actccagcag
    20161 cgagccgtcg ctatcgatgc cgcttatccc cagaatgccg aaatcaaggc ggaactggga
    20221 gataaaatcg agcgtcgctt cgccaatgat cccgccatcg cggctgcgta attcgccacc
    20281 agcgagaata atgcggaaat cttcttttac catcaacgtg ttagcaacat tgagattgtt
    20341 ggtgacaata cgcaaattgc tgtgattaag cagcgcgtgc gccaccgctt ccggcgtggt
    20401 gccgatatcg ataaacagtg tcgagccgtt ggggatttgc tccgccactt tgcgggcaat
    20461 cctctctttt tcttcggtct gggtggcttt gcgatcgtgc cacggcgtgt taaccgaact
    20521 ggaaggcagc gccgcaccgc catggtgacg caggatcagg ttttgctccg ccagctcatt
    20581 gaggtcgcgg cgaatagtct gcgggctgac ggagaaatgc tctaccagct cttcagtact
    20641 gacataaccc tgctgtttaa ccagttcgat aataccgttg tgacgttgtg tttgtttcat
    20701 ttataaatcc ctggaattat tttcgttttc gcgcattgag cgaatcaaca aaagccatcg
    20761 ctaaacccac ggctaacccg gcgatgtgtg ctccgttcgc catcgacatc ccaaacaaat
    20821 caaaccatcc ggcgataatc cagatcagcg caaagataat taacccacgt tgcaggtaaa
    20881 tgccactttg cggatcgcgc tcaccacgta gccagacgta gcccatcagc gcatacacca
    20941 cgccagaaag cccgccaaac cacggcccgc tgaatttttg ctgcacatag ccgcttaaca
    21001 gggcactaat gagagtaata acaattagct taccgctacc gaggcgtttt tccaccgcac
    21061 cgccgagata ccaccaccag agcaggttaa agaggatatg catcagcgag aagtgcatta
    21121 acgcgtgggt gaagtaacgc cagaactcaa atttcagtgt tggatcgaat ggccaggcca
    21181 gccataacat cacttcctga tcgccgagaa tttgcatggc gataaacacc accacgcagg
    21241 cgatcatcac cacccaggtt accggacctg cgcgttcacg caaggcagca aagaaaggat
    21301 aacggcgata atgcagacca ctgccggtat ggcccgcctg ccagctggcc gccagataac
    21361 gcggatctgc cgggttttcg agaaaacgcg ccagctccgc ccgcacgcgc tcggcctggg
    21421 actcatccgc cagccagaca tcgctttggt tatgttgttg aatcgtaagg ataacaccct
    21481 gcgtcgccat gtaatcaaca aacgcctgcg ccacgcgggg gttagcaaaa gaggtaatca
    21541 tcaacatcgt tgctgtcgct tattccacac aaaaggggac agtataaagc gttacgcgcc
    21601 gtacgccacc tctgcgggaa actgacgctg ccaggcttca aaaccgccgt caatgctata
    21661 gaccacatcg tagccctgtt gcagcagata ctgcgccgcg cctttgctgc tattgccgtg
    21721 ataacacatc accatcaccg gagtgtcaaa gtcgttatca cgcataaacg cgcccagcgt
    21781 gtcgttggtt aaatggaaag cctgcaccgc atgtcccatt gcgaaactct gtggatcgcg
    21841 aatatcgacc agcaccgcct ctttttcctg caacttctgg tgcgcgtcgg caacgttaat
    21901 acattcgaac agatccatgc gtctctcttt ctttacaaac aagtgggcaa atttaccgca
    21961 cagtttacgt cgaagcggca gataaacgcc ataatgttat acatatcact ctaaaatgtt
    22021 ttttcaatgt tacctaaagc gcgattcttt gctaatatgt tcgataacga acatttatga
    22081 gctttaacga aagtgaatga gggcagcatg gaaaccaaag atctgattgt gataggtggc
    22141 ggcatcaatg gtgctggtat cgcggcagac gccgctggac gcggtttatc cgtgctgatg
    22201 ctggaggcgc aggatctcgc ttgcgcgacc tcttccgcca gttcaaaact cattcacggt
    22261 ggcctgcgct accttgagca ctatgaattc cgcctggtca gcgaggcgct ggctgaacgt
    22321 gaagtgctgc tgaaaatggc tccgcatatc gccttcccaa tgcgttttcg cctgccacat
    22381 cgtccgcatc tgcgcccggc gtggatgatt cggattggtc tgtttatgta cgatcatctg
    22441 ggtaaacgca ccagcctgcc gggatcaacc ggtttgcgtt ttggcgcaaa ctcagtgtta
    22501 aaaccggaaa ttaagcgcgg attcgaatat tctgactgtt gggtagacga cgcccgtctg
    22561 gtactcgcca acgcccagat ggtggtgcgt aaaggcggcg aagtgcttac tcggactcgc
    22621 gccacctctg ctcgccgcga aaacggcctg tggattgtgg aagcggaaga tatcgatacc
    22681 ggcaaaaaat atagctggca agcgcgcggc ctggttaacg ccaccggccc gtgggtgaaa
    22741 cagttcttcg acgaggggat gcatctgcct tcgccttatg gcattcgcct gatcaaaggc
    22801 agccatattg tggtgccgcg tgtgcatacc cagaagcagg cctacattct gcaaaacgaa
    22861 gataaacgta ttgtgttcgt gatcccgtgg atggacgaat tttccatcat cggcaccacc
    22921 gacgtagagt ataaaggcga tccgaaagcg gtgaagattg aagagagtga aatcaactat
    22981 ctgctgaaag tgtataacgc gcactttaaa aaacagctta gccgtgacga tatcgtctgg
    23041 acctactccg gtgtgcgtcc gctgtgtgat gatgagtccg actcgccgca ggctattacc
    23101 cgtgattaca cccttgatat ccatgatgaa aacggcaaag caccgctgct gtcggtattc
    23161 ggcggtaagc tgaccaccta ccgaaaactg gcagaacatg cgctggaaaa actgacgccg
    23221 tattatcagg gtattggccc ggcgtggacg aaagagagcg tgctgccggg tggtgccatt
    23281 gaaggcgacc gcgacgatta cgcagctcgt ctgcgccgtc gctatccatt cctgactgaa
    23341 tcgctggcgc gtcattacgc acgcacttac ggcagcaaca gcgagctgct gctcggcaat
    23401 gcgggggcga taagcgacct cggggaggat ttcggtcatg agttctacga agcggaactg
    23461 aaatacctgg tggatcacga atgggtccgc cgcaccgacg atgccctttg gcgtcgcaca
    23521 aaacaaggca tgtggctgaa tgcggatcaa caatctcgtg tgagtcagtg gctggtggaa
    23581 tatacgcagc agaagttgtc gctggcgtcg taaggatgat gcccggttga agtgcaaccg
    23641 ggctatttgg attccttaga ctattcattg taaataattg aataacggcc cctgagctaa
    23701 aatcgccaaa cttaagcacg ttacccaatc gccccacttc ttcctctggt ttctattttt
    23761 tctgcaccaa tggcaataat atcccaatgc aaaaccaagc tgtgcactaa tcgggccgac
    23821 aaaatcagtc caaggctgcg gccaaaaagg ggaaaaattc aggggaggcc agacaaacgt
    23881 cagaaagaaa tttcggatat tttccagcaa cgctgatttc tctggagaaa tataaagaat
    23941 gaaattcgag agaattaacc agaaatagac cggcgaaaag accagccagc aatgcagtct
    24001 gttttttaat tcaggaggcg ttctgcggga gaattgccaa ccgatgccag ccattacact
//
LOCUS       DAJVUJ010000051        23486 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.051, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000051 DAJVUJ010000000
VERSION     DAJVUJ010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23486
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.051"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(<1..104)
                     /locus_tag="OQQ48_001847"
     CDS             complement(<1..104)
                     /locus_tag="OQQ48_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HCS1952647.1"
                     /translation="MGSLPLKGNMKQKITDYLDEIYGGTFTATHLQKLV"
     gene            complement(289..603)
                     /gene="pspE"
                     /locus_tag="OQQ48_001848"
     CDS             complement(289..603)
                     /gene="pspE"
                     /locus_tag="OQQ48_001848"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HCS1952648.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(678..899)
                     /gene="pspD"
                     /locus_tag="OQQ48_001849"
     CDS             complement(678..899)
                     /gene="pspD"
                     /locus_tag="OQQ48_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HCS1952649.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(908..1267)
                     /gene="pspC"
                     /locus_tag="OQQ48_001850"
     CDS             complement(908..1267)
                     /gene="pspC"
                     /locus_tag="OQQ48_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HCS1952650.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(1267..1491)
                     /gene="pspB"
                     /locus_tag="OQQ48_001851"
     CDS             complement(1267..1491)
                     /gene="pspB"
                     /locus_tag="OQQ48_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HCS1952651.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(1545..2213)
                     /gene="pspA"
                     /locus_tag="OQQ48_001852"
     CDS             complement(1545..2213)
                     /gene="pspA"
                     /locus_tag="OQQ48_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HCS1952652.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKESGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            2380..3357
                     /gene="pspF"
                     /locus_tag="OQQ48_001853"
     CDS             2380..3357
                     /gene="pspF"
                     /locus_tag="OQQ48_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HCS1952653.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPATVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(3477..4742)
                     /gene="puuE"
                     /locus_tag="OQQ48_001854"
     CDS             complement(3477..4742)
                     /gene="puuE"
                     /locus_tag="OQQ48_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HCS1952654.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(4780..6049)
                     /gene="puuB"
                     /locus_tag="OQQ48_001855"
                     /pseudo
     CDS             complement(4780..6049)
                     /gene="puuB"
                     /locus_tag="OQQ48_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
     gene            complement(6051..7538)
                     /gene="puuC"
                     /locus_tag="OQQ48_001856"
     CDS             complement(6051..7538)
                     /gene="puuC"
                     /locus_tag="OQQ48_001856"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HCS1952655.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLVGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FSDCSDLQKAASSTAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDCAHADSVHSFIREGEGKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEEQALQLANDSQYGLGAAVWMRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(7813..8370)
                     /gene="puuR"
                     /locus_tag="OQQ48_001857"
     CDS             complement(7813..8370)
                     /gene="puuR"
                     /locus_tag="OQQ48_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HCS1952656.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(8397..9160)
                     /gene="puuD"
                     /locus_tag="OQQ48_001858"
                     /pseudo
     CDS             complement(8397..9160)
                     /gene="puuD"
                     /locus_tag="OQQ48_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
     gene            9372..10790
                     /gene="puuA"
                     /locus_tag="OQQ48_001859"
     CDS             9372..10790
                     /gene="puuA"
                     /locus_tag="OQQ48_001859"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HCS1952657.1"
                     /translation="METNIVEVENFVQQPEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDNRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKLLVRLMAEKHKMHSTFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLALNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            10939..11104
                     /locus_tag="OQQ48_001860"
                     /pseudo
     CDS             10939..11104
                     /locus_tag="OQQ48_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11094..12479
                     /gene="puuP"
                     /locus_tag="OQQ48_001861"
     CDS             11094..12479
                     /gene="puuP"
                     /locus_tag="OQQ48_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HCS1952658.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSINLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YDGDRTPVSET"
     gene            12613..12858
                     /locus_tag="OQQ48_001862"
     CDS             12613..12858
                     /locus_tag="OQQ48_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HCS1952659.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVADAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            13171..14814
                     /gene="sapA"
                     /locus_tag="OQQ48_001863"
     CDS             13171..14814
                     /gene="sapA"
                     /locus_tag="OQQ48_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HCS1952660.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            14811..15776
                     /gene="sapB"
                     /locus_tag="OQQ48_001864"
     CDS             14811..15776
                     /gene="sapB"
                     /locus_tag="OQQ48_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HCS1952661.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            15763..16653
                     /gene="sapC"
                     /locus_tag="OQQ48_001865"
     CDS             15763..16653
                     /gene="sapC"
                     /locus_tag="OQQ48_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HCS1952662.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            16653..17645
                     /gene="sapD"
                     /locus_tag="OQQ48_001866"
     CDS             16653..17645
                     /gene="sapD"
                     /locus_tag="OQQ48_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HCS1952663.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVIADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            17647..18453
                     /gene="sapF"
                     /locus_tag="OQQ48_001867"
     CDS             17647..18453
                     /gene="sapF"
                     /locus_tag="OQQ48_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HCS1952664.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            18521..18874
                     /locus_tag="OQQ48_001868"
     CDS             18521..18874
                     /locus_tag="OQQ48_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HCS1952665.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            19242..20030
                     /gene="fabI"
                     /locus_tag="OQQ48_001869"
     CDS             19242..20030
                     /gene="fabI"
                     /locus_tag="OQQ48_001869"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCS1952666.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            20175..21302
                     /locus_tag="OQQ48_001870"
     CDS             20175..21302
                     /locus_tag="OQQ48_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HCS1952667.1"
                     /translation="MEQRHITGKSHWYHETQSSTAEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTARDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESIRWRSSYEV
                     EKLPEEYTKSSTAELCQLAETLSLHPISLSLLERLLNSTRVNTQPDNKLAALLCARIN
                     GSPACFAACMDSSNEYKKISPLLRKGENEINQWADRHSVEHATVQAIQWLTRAPDRFS
                     TAQFSPLLEHEQSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            21370..23304
                     /gene="rnb"
                     /locus_tag="OQQ48_001871"
     CDS             21370..23304
                     /gene="rnb"
                     /locus_tag="OQQ48_001871"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HCS1952668.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGAIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAMRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(23382..23485)
                     /locus_tag="OQQ48_001872"
                     /pseudo
     CDS             complement(23382..23485)
                     /locus_tag="OQQ48_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         5305 a         6205 c         6116 g         5860 t
ORIGIN      
        1 acaagtttct gtaaatgagt tgcggtaaat gttccaccgt agatttcgtc caggtaatca
       61 gtaattttct gtttcatgtt gcctttcagt ggtagcgatc ccatgaaacg agagactact
      121 ccgcagaaaa taaactgtca attgagcaca agggttttag tcacgcagga aatcattttt
      181 taccagggaa aaagcgtatt tgtgagctga cgcaaataat ctatggggat gtaaataagg
      241 tgtgacagaa aaaaaacggc gcataagcgc cgctcatggt gaattctttt aacctttgac
      301 cttcggcatt gcgatgtctt tcaagccacc ggcgttctca acgtgggtat atcccatctc
      361 gctaaggatc tcttttgctt gccctgactg gcgtccggca ttgcaataca ctttcacggt
      421 gtcgttttta tccggaacgg cggtggcaat gcgctctttc acttccttca ggggaatatt
      481 gatggccccc tgaacgtgct cttgctgata ctgctctgga acacgaacat cgatccagtg
      541 ttcagcggcg aaaacgggca gtgaaaacac cagtgccaga gcaagtaagc cttttttaaa
      601 catgtaagcg tccttctatg gatgaaaatt gccatccatt atacgcctga gatttaacaa
      661 tttttgcgaa actcccctca ccttttataa cgctgtgcca gtttattagc agcccgactt
      721 aacagcggtt ccagcgccac agccagcaac attttcagcg gtcggcgagc aactgatttt
      781 atcgcccagc ccgctacacc cgccgggcca tagcgcagtg cggtaagaag taccagcttg
      841 cctgctaatt tgaaaccagg ctttaccttt tgcccggcct gttgccagcg agtattcata
      901 actttcctca cagttgacgg aaacggctac gtaacgtgaa agtatcggaa gtgacataac
      961 gttccatctc gcgtaaacgc gtttcacttg ccgccagttc acggtcgact tcatccagca
     1021 attcgctgct ggagggtagc tgctcaccaa aggccatgtt gtccggcatt ggatcaagcg
     1081 caaatgacaa aatgatgtaa gcaaccaggg taaacagcgc cagaccgaag aaaatcgaca
     1141 gcaccaccag gatacgcacc agttttaccg gtacatcaaa atagttggca atcccggcgc
     1201 agacgccgcg gaccatgccc tgctgtggaa tacgccataa ttttttattg agattaatgc
     1261 ccgccattag cgatccctcc agttcggatg ttctgcatca agaatagatt ccagcgcctg
     1321 aatacgttcg cgcatccgtt ttgcttcatc agccagttgc gctaatcgct gctgctcact
     1381 ttgcgacaat tcactgcgac cagaacgatt gctgtaatgc agccataacc agatcggtaa
     1441 aacaaacagc acaaaaatgg ttaacggaat agccagaaat agcgcgctca taagtactcc
     1501 ttagacgatg agcggcggcg cgttcggacg ccgcctagat gttattattg attgtcttgc
     1561 ttcattttgg cttttaattg tgccagttgt tcgctgattg catcatcggc tttcagttcg
     1621 gcaaactgat cgtccagcga tttttgttta ccgaagctgt ggctttctgc ttccgcttcc
     1681 atctggtcaa tacgacgttc gaaagattcg aaacgagcca ttgcttcatc cagtttgcca
     1741 ctgtccagct gacgacgcac atcgcgcgac gagtttgccg cctgatggcg taacatcaat
     1801 gcctgctggc gagcgcgtgt ttcgctcaat ttgttttcca gctcaccgct ctctttcttc
     1861 atgcgtgcca gcgtatcgtc caccagcgtc acttcatgtt ccagactctt aatcagatcg
     1921 gtcagtttct gtttttcaat taacgccgca cgtgccaaat cctctctctc tttcagcagc
     1981 gccagttcgg ctttttcctg ccattcaact tcacgcgccg acgcttgttc aatacggcga
     2041 gtcagctgtt tcttttctgc caacgcacgc gccgaagtag aacgtacttc aaccagtgta
     2101 tcttccatct cctggatcat cagacgcacc agtttctgtg gatcttccgc tttctctaac
     2161 agagcgttga tgttggcatt cacgatgtcg gcaaagcgag aaaaaatacc cataatgttg
     2221 tcctcttgat ttctgcgttc aggattgtcc tgctgaactg ttaatacaat ttgcgtgcca
     2281 attttttatc tttttgattt ataaagatct gattgaagaa tcatcagcaa catgccagga
     2341 tgaattagcg aattacacta acaagtggcg aatttcatca tggcagaata caaagataat
     2401 ttacttggtg aggcgaacag ctttctcgaa gtgctggaac aggtttcgca tctcgcaccg
     2461 ctggacaaac cggtgctcat catcggcgaa cgcggcaccg gtaaagagct gattgccagc
     2521 cgcctgcatt atctctcctc ccgttggcaa gggccgttta tttcccttaa ctgcgcggcg
     2581 ttaaatgaaa atctgctgga ttccgaactg tttggtcacg aagcgggggc gtttaccggt
     2641 gcgcaaaaac gtcatccagg gagatttgaa cgtgccgacg gcggtacgct atttcttgat
     2701 gaactcgcta cggcaccgat gatggtgcag gagaaattat tgcgcgtgat tgagtacggt
     2761 gaactggagc gcgttggcgg cagccagcca ttgcaggtga atgtgcggtt ggtatgcgcg
     2821 acgaatgccg atctcccggc gacggtcaat gaaggcactt ttcgcgctga cctgctcgac
     2881 cgactggctt ttgatgttgt acaactgcca ccactgcgcg agcgcgaaag cgacataatg
     2941 ttgatggcag aacactttgc catccagatg tgtcgggaaa tcaagctgcc tctgttcccg
     3001 gggtttacgg agcgcgccag agaaacattg ctgaattatc gttggccggg aaatattcgt
     3061 gaattgaaaa acgtggtgga acgttcagtg tatcgccacg gcaccagcga ttatccgctt
     3121 gatgacatca ttattgatcc ctttaaacgg cgtccgcctg aagacgctat cgccgtttca
     3181 gaaaccacct cgcttccaac actgccgctg gatttacgtg agtttcagat gcagcaggaa
     3241 aaagagttgc tgcaactcag tttgcaacaa gggaaatata accagaagcg cgcggctgaa
     3301 ttactggggt taacttatca tcagttccgc gcgttgttga aaaagcacca gatttagcga
     3361 agttgagcaa tacaacttgc cggatgcggc gtgaatgctt tattcggcat acataatcaa
     3421 taagtcgttt gcaggcctga taagcgcagc gcatcagaca ttattgcgtt gggctattaa
     3481 tcgctcagcg catcctgcaa aattttcatt gccgcatcga attgcgcatc cggaatggtc
     3541 agcggataca ggaagcgaat cacgttgccg tatgcgccac aggtcagcag gagcagcccc
     3601 tgcgccagcg cgcgttgctg gattttctgt gcaatcgccg ctgacggctc gcccgtttgc
     3661 ggatcgttaa actctgccgc aatcatcgac cccaggccgc gtaccgcagc aatggccgga
     3721 acgctttctt tggcatcaat caacgtgttt ttgagacgct ggcccagttg attcgcgcgt
     3781 tcgcagagtg attctttgtc gataatattg agcaccgcgt gcgcggcagc caccgccagc
     3841 gggttcccgg cgtaggtgcc acccaacccg cccggcgcgg gcgcgtccat aatattcgcg
     3901 ttaccgacca cgcccgaaag cggcatcccg cccgcgaggc ttttcgccat cgtcattaag
     3961 tccggcttat cggcgtaatg atccatggca aacagtttac cggtacgcgc aaagccgctt
     4021 tgcacttcat cggcaatcat cacaataccg tgctcgtcgc acaggcggcg aatggcggca
     4081 accagctctt ttggcgcaac gttgaaaccg ccctcgccct gcaccggttc gaaaataatg
     4141 gccgccacct gcttcgcttc aatgtccgat ttaaacaagc gttcgatggc gtcgagggag
     4201 tcctgtgttg aaatgccgtg taaatctgac ggataaggta cgtgatacac cgaaccaggg
     4261 aacgggccga agccgatttt gtacggcgca acttttccgg tcagcgccat ggtcatatat
     4321 gtacgaccgt ggaagccgcc gctaaacgca atcacgccag gtcgtccggt atgggcgcga
     4381 gcaattttca ccgcgttttc caccgcttcc gcgccggtag tgaagaatgc ggttttagcc
     4441 tgcccgctca ctggggcaag ggcgttaatt ttctccgcca gcgtgacgta gctttcgtac
     4501 ggcacaatct gatacgccgt gtgggtaaac tgttgcaatt gctgctccac cgccgcgacc
     4561 agatcagggt ggcgatgccc ggtattcagc accgcaatgc ctgcggcgaa atcgatgtat
     4621 tcgttgccct ctacgtcctt cagcgtagcg ttttctgccg actgggcgaa gaagttacac
     4681 atcacgccaa ccccgcgtgg ggtagcagaa agacgacgct gatggaattc attgttgctc
     4741 atgtttcaca ccttttgact cttcactgtt aatcggaaat taaacgccca gacgatcgcg
     4801 caggctgtaa taagccgcgc ccatcgcggt aaatggcacg cgcagcgtgc gcccgccggg
     4861 gaacgggtaa tgcggcagat tggcgaaggc atcgaaacgc tcggcgtcac cgcgcagcag
     4921 ttcagcaatc aaacgcccgg cgaggtgggt acaggtgacg ccgtggccgc tgtagccctg
     4981 catgtaatag atgttggtat cgaggcgacc gaactgcggc atccgtgaaa gggtcagcag
     5041 gaagttgccc gtccagcgat agtcgatttt gacgcccttc agctgcggga aggttttcag
     5101 cagtttcggc acaacaagac gctcaacgtc atccgggtcg cgcgcgccgt agaccacgcc
     5161 gccgccgtac agcaaacggt tgtcggcggt aagacggtag taatcaagca gataattaca
     5221 gtcttccaca cagtagtttt tcgggatcag cgaacgggct aaatcttccg acagccgttc
     5281 ggtggtgatc acctgcgtgc cgcacggcat gctgcgtttc gccagttccg gctctacttt
     5341 atcgcccaga tacgcattcc cggcgacaat cacatacttc gccgtcacct gacctttggc
     5401 agtgcgcaca acggctggcg tggtgtgctg gatctgcgtc acggcagaaa gttcatacac
     5461 gcgcccgccg ttgaggcgga tggcgtccgc ttcgccaatg gcgaggttta gtgggtgaat
     5521 atgcccaccg ctgtgatcca gcagcgcacc ggtatagcga tcgctggcta cttcacggcg
     5581 aatggcgttg gcgtccagca attccagctg tttattgccg tagcgttccc agttctcttt
     5641 ctgctcttca agtgtggcga gctgtttatc attcatcgcc acaaacaggc tgccggggcg
     5701 gtagtcgcag tcaatctgat aacgtttgat acgttcgcgg atgatctcac caccttcgaa
     5761 catcatgctg ccgagcatag gggcggtgtc catgccgtag cttttttcga tcacgtcgat
     5821 gtcgcggttg taggagttca caagctgccc gccattgcgc ccgcttgcgc caaagccgat
     5881 gcgtgaggct tcgagaacca ctacgtcaaa gcccgcttcc gccagatgca gcgcggagga
     5941 gagcccggta tagccgccgc caaccacgca aacgtcgcag gtgattgact cattcagggt
     6001 gtcgaatggc acgtatttat gcgtaataac tgctggtatg ttcggtcatt tcaggcctcc
     6061 aggcttatcc agatggtttt cagttcggtg aatttttcaa gggcatgcag ggatttgtcg
     6121 cgaccgttgc cgctctgctt atagccgcca aacggcacgg tcatatcgcc gtcgttgtag
     6181 ttattgacga agacggaacc ggctttcagg cggcggctca tacggtgcgc gcgggagagg
     6241 tcgcgcatcc ataccgccgc gccaaggccg tactggctgt cgttggcaag ctgtagcgcc
     6301 tgttcttctg atgtgaaacg cgtgaccacc agcaccggac cgaaaatctc ttcgcggctt
     6361 aaggacgcat tcgggtctac atccacaaag atggtcgggc cgatggcggc agccagcccg
     6421 gcgttacggc catccagcag cagttgccct ttgccttcgc cctcacgaat aaagctatgg
     6481 accgagtcgg cgtgggcgca gtcgattaaa gtgcccatgg tggttgcggg gtcaagcgga
     6541 tggcccggct gccagttttg cgcctgctgt tttaacaggg ctaagaattc atcggcgatg
     6601 ctctcttcca gcaacaggcg tgttccggcg atgcacacct gaccctggtt gtagaagatg
     6661 cctgctgcgg tggagctcgc cgctttttgc agatccgaac aatcggagaa aacgatgttg
     6721 gcgcttttgc cgcccgcttc cagccagacg cgtttcatgt tgctgtcgcc cgcatctttc
     6781 agcagctgtt tcccggtacg ggttgaaccg gtaaaggcaa tggcgtcgat atcgttatga
     6841 cgcgacagcg cctgccccgc ttcatgacca aaacccgtca ccacgttcaa cacaccatcc
     6901 ggcaagcctg cttctttcgc cagccccacg agacgaatcg cactgagcgg tgatttttca
     6961 gacggtttta gaatcacgct gtttcccgcc gccagcgccg ggccgagttt ccagcaagtc
     7021 agcaacagcg ggaagttcca cggcacgatg gcggcaatca cgccgaccgg ttcacgcacg
     7081 atcatcgcca gctcatggct actggtggtc gccacttcgc catacacttt gtcgatcgct
     7141 tcggcgtacc agcgaatggc gcgcgccgcg ccgggaatat catcacgcag actgtgacga
     7201 atcggtttgc cggtgtcgag agtttccagc agtgccagct cttcggcgtg ggcttccatt
     7261 aaatcggcga gtttattcag taccgcttta cgtttagccg gagaagagag tgaccagtcg
     7321 ccgcgttcaa atacgccgcg tgctgcgctc accgcacggt cgatatcgac gctcttgccg
     7381 cgggcaattt tcgccagcgg tgcctgggtg actggatcaa cggtttcaaa ggtttcattt
     7441 tccgccgcag cagtatattc accgttaata aataagcggt tttcaatggc gagacttaac
     7501 gctttatcct gccagtaagc cagatgatga aaattcatta tgactcctgt ttcacgtcta
     7561 tcagatatat gcagtttgtg aacgtcaggg cgcagagatc cccgggccgt atgcccgttt
     7621 atttctggcg acattcttta tatacacttc atccttcaag ccgcctctgc cttggctgcg
     7681 ttcgctcccc cgaatcactt acctgagtaa gctcatcggg attcgctcac ttgccgcctt
     7741 gatgcaactt gaatgatttt gtgtatagat tgccgggcgc attgcgctta cccggcttac
     7801 agaacaaaaa gattaaaacg tggtgggcgt atgggcgctg ataattcggc aaatacctgc
     7861 cgacgtatta ctgaaactgt gcgggatgcc ggtattaatg gcataacttt gacccgcgac
     7921 gaggtggtaa tcctgaccat taatcgtcag aacaatttca ccttccagta ctgtgcctat
     7981 ttcctcaccc tgatgcttaa ttctttcccc agtggttgtg cccggctggt acgtttcaaa
     8041 gatcatcgcc agcgtgcgat tcgggttacc gttatgaacc agcttcattg acacaccctg
     8101 actgcccatc tcaattaagt cgtcctgatt aatgacgacc tgcggctcat caggtttttc
     8161 cggctcggaa aagaattccg agagtgacag accatacacc ttcagcagct tttgcagcgt
     8221 actgatggca gggctgactt tatcttgttc tatcgtactg atagcactgt gagtcagccc
     8281 ggagagttcg gcggcacgac gttgtgaaag cccctgctgc tggcggattt ccgacaagcg
     8341 ttttcctggc gccagtccct catcactcat atttgcattt ccttaaactg tagtggtcag
     8401 agtcgctgtt tttcagcgat atggtgctga caagcggtga taaagccctc gaacaatata
     8461 cgcgaaagag cgtactcgct actgttccat tccgggtgcc actgtacgcc cagcgcaaaa
     8521 ggatgattga tgacgctaac cgcctcaact aagccatccg gcgagcgagc ttcaacacgc
     8581 aaccgtgggc taacgacctt cgccccttgt ccatgtagag agtttaccca aaagttgcta
     8641 cattcaggta acaacgcaga cagtaatccc ccctcttcaa cctgaacttc atgcgacggt
     8701 gcatattgct gttccaccgg cagttcggat cttcccgatg ttccagcaat tcaggctgtt
     8761 cgcacagctt gcgatgcaac gaaccaccgg ttgccaccac cagttcttgt aaaccccggc
     8821 agatggcgaa aatggggatg cgcctttcga gtgcggcatt aattatcgcc atgctcagaa
     8881 gatcacgccc gggatcggcg tcaggctcat cgccgttttc accatatagg tgcggctgca
     8941 cattgctggg actaccagga agataaatgc catcgagttt cggcaaaagt tgttcaagta
     9001 acgacggttc cgccagcgca tgtggtagcg caattggcaa gccgcctgcg tggatgatgg
     9061 cattcaggta cttttcttgc agagtctggg tcgcatgacc cttaagcctg ttcctgcaca
     9121 ttacgacacc gataaccgga ttgttcatta tatttttcat gctcaatctc acaaagtgga
     9181 ctaaattatc gccattactg cgctttaaat gacctttatg ttcaatattt tttcaatcta
     9241 gcagtgggtt ttcatttttg caaactaaat ttaacatttg gcaaacattt agtttgcata
     9301 cagattcgaa tggtggtcat tatattttac gctttgataa cgagcggaaa acaaaccaaa
     9361 ggcgaagaat catggaaacc aatatcgttg aagtagagaa ctttgttcag cagccagaag
     9421 agaggcgggg tagcgccttt acgcaggaag tgaaacgcta cctggagcgc tacccgaata
     9481 cgcaatatgt tgatgtttta cttactgatt tgaatggttg cttccgtgga aaacgtatcc
     9541 ctgtttcaag cctgaagaag ttggagaaag ggtgttattt cccggcctcg gtgtttgcaa
     9601 tggatattct gggcaacgta gtagaagaag cgggtctggg tcaggaaatg ggcgagccgg
     9661 atcgtacttg tgttcctgtt ctcggttcct taaccccttc tgccgccgat ccagagttta
     9721 tcggtcagat gctcctgacc atggtcgatg aagatggcgc tccctttgac gttgagccgc
     9781 ggaacgttct taaccgcctg tggcagcagc tacgccagcg cggattgttc ccggttgtag
     9841 cggtagagct ggagttctat ttactggatc gtcagcgcga cgctgaaggg tatctgcaac
     9901 cgccctgcgc gcccggcacc gataaccgca atacacaaag ccaggtttac tccgttgata
     9961 acctcaacca cttcgccgac gtgctcaatg atattgatga actggcgcag ttacagttaa
    10021 ttcccgccga cggtgcggtc gccgaagcct cgccgggtca gtttgaaatc aacctttacc
    10081 ataccgataa cgtgctggaa gcctgcgatg atgcgctggc gctaaaactc ctggtgcgtc
    10141 tgatggcaga aaagcataag atgcactcca cttttatggc gaagccgtat gaagagcacg
    10201 cgggcagcgg gatgcatatc catatcagta tgcaaaataa tcgtggcgag aatgtgcttt
    10261 ctgacacaga aggcgaagat tcgccgctgc tgaaaaagat gctcgccggg atgattgacc
    10321 tgatgccgtc gtcgatggcg ttgctggcac taaacgtgaa ctcgtatcgc cgcttccagc
    10381 cgggaatgta tgtgccgacg caggcgtcgt ggggccataa caaccgcacc gtcgccctgc
    10441 gtattccgtg cggcgaccgt cataatcacc gcgtggaata tcgcgtggcg ggtgccgatg
    10501 ccaacccata tctggtgatg gcagcgattt ttgccggtat tttgcatggc cttgataacg
    10561 agctgccgtt gcaggaagaa gtcgaaggca acgggctgga acaggaaggc ttacccttcc
    10621 cgattcgcca gagcgatgcc ctgggtgagt ttatcgagaa tgatcacctg cgccgctatt
    10681 taggcgaacg gttttgccat gtgtatcacg cctgtaaaaa tgatgagctg ttgcagtttg
    10741 agcgcctcat cacagaaaca gaaattgagt ggatgttgaa aaacgcatag taccccttgc
    10801 ggggttgttt catcgacctg agcggagcca ggcctgacat ttgctggtaa tccagacatt
    10861 tcccggatgt cgcgttatgg agcgcaggcg gcaacgggct tccgttaacg attaagactg
    10921 tgacgaggaa atgagatgat gccgatgatt aaatccccgc atggcgaggg aggctgcgta
    10981 tgcgcccctc ctgctactga ctggactccg tcccctttgc ttcccttgct aaacaggttt
    11041 gatttcaggt cgacacgacc gcaaacctta ttacgcaggg ggaggcagca attatggcta
    11101 ttaattcacc actgaatatt gctgcgcaac ccggcaaaac ccgtctgcga aaatcactga
    11161 aattgtggca ggtggtgatg atgggtctgg cctatctcac gccgatgacc gtatttgata
    11221 cttttggtat tgtctccggc attagcgacg gtcacgttcc ggcgtcctat ttgctggcgc
    11281 tggcgggcgt gctgtttacc gctatcagct acggcaaact ggttcgccag tttccggagg
    11341 ccggttcggc ctatacctac gcgcaaaagt cgattaaccc gcacgtcgga tttatggtcg
    11401 gctggtcatc gctgctggat tatctctttt tgccgatgat caacgtcttg ttggcgaaaa
    11461 tctatctctc cgccctcttc ccggaagtgc cgccgtgggt gtgggtagta accttcgtcg
    11521 ccattttaac cgccgcgaat ctgaagagca tcaacctggt cgctaacttc aataccctgt
    11581 ttgtactggt gcaaatctcc atcatggtgg tgtttatctt cctggtagtt cagggactgc
    11641 ataaaggaga aggcgttggc accgtctggt cacttcagcc gtttatcagc gagaacgcgc
    11701 acctgatccc gattattacc ggggcgacga ttgtctgttt ctccttcctc ggtttcgatg
    11761 cggtgaccac gctttcggaa gagacgccgg acgccgcacg cgtgatcccg aaagccatct
    11821 tcctgacggc ggtctacggt ggcgttatct ttatcgcggc gtcgttcttt atgcagctgt
    11881 tctttcccga tatcagccgc tttaaagatc cggatgccgc actgcctgaa attgcgctgt
    11941 acgtcggcgg caagctcttc cagtcgattt tcctctgcac cacgtttgtg aacacgttag
    12001 cctctggcct ggcttcacat gccagcgtgt cgcgtctgct gtatgtgatg gggcgtgaca
    12061 atgtgtttcc ggagcgcgtg tttggctatg tgcacccaaa atggcggact ccggcactca
    12121 acgtcattat ggtcgggatt gtcgcgttgt cggcgctgtt cttcgattta gttaccgcca
    12181 cagcgttaat taacttcggt gctctggtgg cgtttacctt cgtgaatctg tcggtgttta
    12241 atcatttctg gcggcgtaaa ggaatgaata aaagctggaa ggatcacttc cactatttgc
    12301 tgatgccgct ggttggcgcg ctgacggtgg gtgtgctgtg ggttaacctc gagtcaacgt
    12361 cactgacact cggtctggta tgggcttcgt taggcggcgc atatttatgg tatctgatcc
    12421 gccgctatcg caaagtgccg ttgtatgatg gtgacagaac gccggtgagt gaaacgtaat
    12481 agtcggatgc gcggcgcatt atcctgccgg agggtaatgc accgcgccat taactataaa
    12541 aagatgtatg acccggatca cgagtgctat catccgccct tatgcttagc gcattaaccc
    12601 caggggcaaa taatgaacca cgatatcccg ctaaaatatt ttgatattgc cgatgagtac
    12661 gcgaccgaat gcgcagagcc tgtcgcagac gctgaacgca caccgctggc ccactacttc
    12721 cagttactgc tcacccgttt gatgaacaat gaagagatca gcgaagaagc ccaacatgaa
    12781 atggctgctg aagcggggat taatcccgta cgcattgatg agatcgcaga gtttttgaat
    12841 caatggggta atgagtaatt atttaattgc agacgatcgg tcagtatcgt tgtccggata
    12901 aagcgttcac gccgcatccg gctaacaatg acacgcactg cagcgtgctt taatgggttt
    12961 ctcacgatcg taccgctctg ctcctcgcta agttgttctg tgagttatag ttatagagga
    13021 agcacgggta cgaatgttga acagatggcg acgccacctg ttcaacgacc aaccacacgg
    13081 ccctcattac gacccctgaa cacaaacggt aatggcaaat gtcattaaat attagtaaga
    13141 tagcctcttt acataaaatc ccttaatatt atgcgccagg tattatcgtc tcttttggtg
    13201 attgctggac ttgtgagtgg tcaggcaatc gccgcgcctg aatccccccc gcatgctgat
    13261 atccgcgaca gcggttttgt ctattgcgtc agcgggcaag tcaacacctt taacccatcc
    13321 aaagcgagca gtgggttaat tgtcgatacc cttgccgccc agttttatga tcgactgctg
    13381 gatgtcgatc cctataccta tcgcctgatg ccggaacttg ccgaaagctg ggaagtactc
    13441 gacaacggcg cgacctatcg cttccacctg cgtcgcgatg ttccgtttca aaaaaccgac
    13501 tggtttactc ccactcgtaa aatgaatgcc gacgatgtgg tgtttacctt ccagcgaatt
    13561 tttgaccgca acaacccgtg gcataacgtc aacggcagca acttccccta cttcgacagc
    13621 ctgcaatttg ccgataacgt gaaaagcgtc cgcaaactgg ataatcatac cgttgagttc
    13681 cgactcgccc agccggatgc ttcttttttg tggcacctcg caacccatta tgcttcggtc
    13741 atgtcggcag aatatgcccg gaagttagag aaagaagatc gccaggagca actcgaccgt
    13801 caaccggtcg gcaccggtcc gtatcagttg tcggaatacc gcgccgggca atttattcgc
    13861 ctacaacgtc atgatgactt ctggcgcggt aaaccgttaa tgccgcaggt agtggtggat
    13921 ttaggctccg gcggcaccgg acgtctgtcg aaactcctga ccggggaatg cgacgttctg
    13981 gcctggcctg ctgccagcca gctatccatt ttgcgtgacg acccgcgctt gcgtttaacg
    14041 ctgcgtcctg ggatgaacgt cgcctatctg gcatttaaca ccgccaaacc gccgctaaat
    14101 aatcccgctg tccgccatgc actggcactg gcgattaata accagcgcct gatgcaatcc
    14161 atctattatg gtacggctga aacggcggcc tctattttac cgcgcgcctc gtgggcctat
    14221 gacaacgagg ctaaaattac tgaatacaat ccggcgaaat cgcgcgaaca gttgaaggcg
    14281 ttggggctgg aaaacttaac gctgaaactg tgggtgccca cacgttcgca ggcgtggaac
    14341 cccagtccac tgaaaactgc cgaactgatt caggcggata tggcgcaggt tggcgtaaaa
    14401 gtggtgattg tgccggtaga aggtcgcttt caggaggcgc ggttgatgga tatgagccat
    14461 gatctgacgt tatccggttg ggcgacggac agtaacgacc cggacagttt cttccgtcct
    14521 ttactgagct gcgcggcaat tcattcacag accaacctcg cccactggtg cgatccgaaa
    14581 ttcgacagcg tgttgcgtaa ggcgctctcc tcgcagcagc tggcggcgcg tattgaagcc
    14641 tatgacgaag cgcagagtat tctggcgcag gaattgccca ttttgccgct ggcgtcgtca
    14701 ttgcgtttgc aggcctatcg gtacgatatc aaaggtctgg tacttagccc gtttggtaac
    14761 gcctcctttg ctggggtgta tcgcgagaaa caggatgagg tgaaaaaacc atgattatct
    14821 tcaccttacg ccgcattttg ttattgattg tcaccttgtt cctgctgacc ttcgttggct
    14881 tcagcttgag ctatttcacc cctcatgcgc cgttacaagg cgcgtcactg tggaatgcct
    14941 gggtgttctg gtttaacggc ctgatccact gggattttgg tgtttccagc attaacggtc
    15001 aaccgattgc cgaacagtta aaagaggtct tcccggcgac gatggaactg tgcatcctcg
    15061 ccttcggttt tgcgctgatt gttggcatcc ccgtggggat gattgccggg attacgcgcc
    15121 ataagtggca ggataacttg atcaacgcca tcgccttact gggcttttca atcccggtgt
    15181 tctggctggc gcttctgttg acgctgtttt gttcactcac gctaggttgg ttgcccgttt
    15241 cagggcgttt cgatctgctc tacgaagtga aaccgattac cggttttgcg ttgattgatg
    15301 cctggctttc ggactcacca tggcgggatg aaatgatcat gagcgcaatc cgccatatga
    15361 tattgcccgt gatcaccctg tcggttgcgc caacaactga agtgatccgc ctgatgcgta
    15421 tcagcactat cgaagtgtat gaccagaact atgtaaaagc ggcggcgacc cgcggtttgt
    15481 cgcgctttac tattttgcgt cgccacgttc tgcataacgc gttaccgccg gttattcctc
    15541 gcctgggctt acagttttcc accatgctga cgctggcgat gatcaccgaa atggttttta
    15601 gctggccggg tttagggcgc tggttaatta acgccatccg ccagcaggat tacgcagcca
    15661 tttccgccgg agtgatggtg tgtggctcac tggtcattat tgttaacgtg atttctgata
    15721 ttttgggtgc catggctaac cctctgaaac ataaggaatg gtatgcctta cgatagcgta
    15781 tacagcgaaa agcgcccgcc gggcacgctg cgtaccgcct ggcgcaaatt ttatagtgac
    15841 gcctctgcga tggtcggcct gtacggctgc gcggggctgg ctgtactgtg tatttttggc
    15901 ggctggtttg cgccttacgg catcgaccag caatttctcg gttatcaatt attgccgcca
    15961 tcatggtcgc gctatggcga agtttctttc ttcctgggga ctgacgacct cggacgcgat
    16021 gttttaagcc gtttactgag cggagctgcg ccaaccgtgg gtggcgcatt tgtggtgacg
    16081 cttgccgcga cgatctgtgg cctagtactg ggcaccttcg ccggggcgac acacggcctc
    16141 cgctcagcgg tgcttaacca tattctggat accttactgg cgatcccttc gctgctgttg
    16201 gcaattatcg ttgtagcgtt tgccggaccg agtttgtccc acgctatgtt tgctgtctgg
    16261 ctggcattgc tgccgcgtat ggtacgttcg atttacagca tggtgcatga cgaactggaa
    16321 aaagagtacg ttatcgccgc ccgtctggat ggcgcatcaa cgctgaatat tctctggttt
    16381 gctgtgatgc caaacatcac cgccgggctg gtcaccgaga tcacccgcgc actgtcgatg
    16441 gcaattctcg atatcgccgc gctgggcttt ctcgatctcg gcgcacaact cccctcgcct
    16501 gaatggggag cgatgctcgg tgatgcgctg gaactgattt atgtcgcccc gtggactgtc
    16561 atgctgccag gtgcggcaat tatgattagc gtgttgttgg ttaacctcct tggcgatggc
    16621 gttcgtcgtg cgattattgc gggggtggaa taatgccatt actcgatatt cgtaacctga
    16681 ccattgaatt taaaaccggt gatgagtggg ttaaagccgt cgaccgggta agtatgacct
    16741 taaccgaagg tgaaatccgc ggtcttgttg gtgaatccgg ttccggcaag agtttgattg
    16801 cgaaagcgat ttgtggggtg aataaagata actggcgtgt tattgctgac cgtatgcgtt
    16861 ttgatgatat cgatttgctg cgtctctccg cacgcgaacg gcgcaaactg gttggccata
    16921 acgtgtcgat gattttccag gaaccgcagt cgtgtcttga cccttcagaa cgtgtgggtc
    16981 gccagttgat gcaaaacatc ccagcctgga cctataaagg ccgttggtgg cagcgttttg
    17041 gctggcgcaa acgccgtgcc attgaactgc tgcaccgcgt ggggattaaa gatcacaaag
    17101 atgcgatgcg cagttttccc tatgagttga ccgaaggtga atgtcagaaa gtgatgatag
    17161 ccattgcact ggcgaatcaa ccgcgtctgc tgattgctga cgaaccgacc aactcaatgg
    17221 agccaacaac ccaggcgcaa atctttcgcc tgctgacgcg tctcaaccaa aacagcaata
    17281 ccactatttt gcttatcagc catgacttac aaatgcttag ccaatgggcg gataaaatta
    17341 acgtgcttta ctgcggtcaa acagtggaaa ccgcgccgag taaggagttg gtgacgatgc
    17401 cacatcatcc ttatacccag gcgctgatcc gcgcgatacc agacttcggc agcgcgatgc
    17461 cgcataaaag tcgcctcaat acgctgcccg gcgctatccc actgctggaa cagttaccga
    17521 ttggttgtcg tctggggcca cgttgcccgt atgcacaacg agaatgcatt gtgacgccac
    17581 gtttgacggg ggcgaaaaat catctctatg cctgtcattt cccgctgaac atggagaaag
    17641 agtgagatga tcgaaacgct gcttgaagtg cgtaatttaa gtaaaacgtt ccgctaccgg
    17701 accggctggt ttcgccgtca gaccgtagaa gcggtaaaac ccttgagctt tacgctacgt
    17761 gaaggccaga cactggcgat tattggcgag aatggttcgg gtaaatccac gctggcaaaa
    17821 atgctggcgg gaatgataga gcctaccagc ggcgagttat tgattgacga tcatccactg
    17881 cattttggcg attattcctt ccgcagtcag cgtattcgca tgatttttca ggatccttcg
    17941 acctcgttga atccccgtca acgtatctcg caaattctgg attttccact gcgcctgaac
    18001 accgacctgg aaccggaaca gcgtcgcaaa cagattattg aaacgatgcg tatggttggg
    18061 ctgctaccgg atcacgtcag ttactatccg catatgttgg cacccggaca aaaacagcgt
    18121 ctgggtctgg cgcgcgcgct gatattgcgc ccaaaagtca ttattgccga tgaagcactc
    18181 gcttcactgg atatgtcgat gcgttcgcag ttgattaatc tgatgctgga gttacaggaa
    18241 aaacagggca tttcgtatat ttatgttacc cagcatatcg gaatgatgaa gcacattagc
    18301 gaccaggtgc tggtgatgca tcagggcgag gttgtcgagc gaggcagcac cgcggatgtg
    18361 ctggcatcgc cgctgcatga actcaccaaa cggctgattg ccgggcactt tggcgaagca
    18421 ttgacagcgg atgcgtggcg taaagatcgc taattttctg aatcctcctt ccagattatc
    18481 cacctcattc tgacaagaga ttaaatcttg tcagaatgag gtgatggata aaataaaatc
    18541 aaatgcgcgt gatttaagac gcaatctcac tttgcaggaa cgaaagctct ggcgatatct
    18601 tcgcagccga cgttttggtg atttcaaatt tcgccgtcaa catccggtgg ggagctacat
    18661 tctcgatttt gcttgctgct cggcgcgcgt agtcgttgag ctggatggtg ggcagcatga
    18721 tttggcagtt gcctatgata ccaggcgcac tagctggctt gagtcgcagg gctggaccgt
    18781 gctgcgtttc tggaataacg agattgattg taatgaggag gcggtgctgg agattattct
    18841 gcaggaactg aaccgccgat caccctcacc ctgaaagggc gagggggccg accgagccga
    18901 atagctgttg tggtgaaaac atggagactg tgctggagaa tattcggcaa ggtctgaacc
    18961 atcccagcca tcgccataaa agggttaggg gctgtatgag cctgtttgtt gctggggtaa
    19021 caatatttgc acaatatggt cccctcgccc ctctggggag agggttagtg taaggggaaa
    19081 agcgcccacc ctgccgcagc ctgctccggt cggacctagc aactatagct actcacagcc
    19141 aggttgatta taataaccat ttatctgttc gtactgttta ctaaaacgac gaatcgcctg
    19201 attttcaggc acaacaagca tcaacaataa ggattaaagc tatgggtttt ctttccggta
    19261 agcgcattct ggtaaccggt gttgccagca aactatccat cgcctacggt atcgctcagg
    19321 cgatgcaccg cgaaggagct gaactggcat tcacctacca gaacgacaaa ctgaaaggcc
    19381 gcgtagaaga atttgccgct caattgggtt ctgacatcgt tctgcagtgc gatgttgcag
    19441 aagatgccag catcgacacc atgttcgctg aactggggaa agtttggccg aaatttgacg
    19501 gtttcgtaca ctctattggt tttgcacctg gcgatcagct ggatggtgac tatgttaacg
    19561 ccgttacccg tgaaggcttc aaaattgccc acgacatcag ctcctacagc ttcgttgcaa
    19621 tggcaaaagc ttgccgctcc atgctgaatc cgggttctgc cctgctgacc ctttcctacc
    19681 ttggcgctga gcgcgctatc ccgaactaca acgttatggg tctggcaaaa gcgtctctgg
    19741 aagcgaacgt gcgctatatg gcgaacgcga tgggtccgga aggtgtgcgt gttaacgcca
    19801 tctctgctgg tccgatccgt actctggcgg cttccggtat caaagacttc cgcaaaatgc
    19861 tggctcattg cgaagccgtt accccgattc gccgtaccgt tactattgaa gatgtgggta
    19921 actccgcggc attcctgtgc tccgatctct ctgccggtat ctccggtgaa gtggtccacg
    19981 ttgacggcgg tttcagcatc gctgcaatga acgaactcga attgaaataa tcattctgtt
    20041 ggtaaatatt gggcggcgtt ctgccgcccg ttatctctgt tatacctttc tgatatttgt
    20101 tatcgccgat ccgtctttct ccccttcccg ccttgcgtca ggataacgat ttcctttacg
    20161 accaaggagc gcccatggaa caacgccaca tcaccggcaa aagccactgg tatcatgaaa
    20221 cgcaatccag tactgcggag tatgacgttc tgcctctggt cccggaagcc gcaaaggtca
    20281 gcgatccctt tctgctcgac gtgatccttg atgaagaaac gctagctccc ttcctttcat
    20341 ggctggtccc tgcgcgcgtt cttgcagtgg aattgttccc tgaccagctt accgtgaccc
    20401 gttcacagac cttcaccgct tatgaacgct tgtctacggc cctgacggtt gctcaggttt
    20461 gcggcgtcca gcggttatgt aactactatt cggcgcgact tacgccgctc cccgggcctg
    20521 attcctctag ggaaagcaat cataggttgg cacaaatcac gcaatatgcc cgccaactgg
    20581 ctagctcgcc ttctattatc gacaaccgat cgcgccagca tctgaatgac gtcggtctta
    20641 ctgccaggga ctgtgtaatc attaaccaaa tcattggttt tattggcttt caggcgcgga
    20701 ccattgcgac atttcaggct tatctcgggc acccggtacg ctggttaccc ggtctggaaa
    20761 tacaaaacta cgccgacgcg tcactgtttg ctgatgaatc aatacgctgg cgaagcagct
    20821 atgaggtgga aaaactacct gaagagtaca caaaaagttc aacagcagaa ctttgccaac
    20881 tggccgaaac actctctctc caccctattt cactttccct tctcgaaagg ttgttaaaca
    20941 gtacacgggt taatacacag ccggataata agcttgcggc gttgttatgc gcgcggataa
    21001 atggcagtcc tgcttgtttt gccgcctgta tggattcatc aaatgaatat aaaaaaatca
    21061 gcccccttct gcgcaagggc gaaaatgaaa ttaaccaatg ggctgaccgt cattctgttg
    21121 agcacgctac cgttcaggcg atacaatggc tgacccgagc acccgatcgc tttagcaccg
    21181 cccagttcag ccctttactc gaacacgaac aatcatcaac gcagattatt aatctgctgg
    21241 tatggagcgg gctgtgtggc tggataaatc gcttaaaaat cgcgttgggt gagacatatt
    21301 aaccttgccg cgtcagacag attcgcgtaa aactgtcagc cgctctaatg gccaccaaaa
    21361 tagacaatta tgtttcagga caacccgctg ctagcgcagc ttaaacagca actgcattcc
    21421 cagacgccac gcgctgaagg ggtggtaaaa gccacagaaa aaggctttgg cttcctggaa
    21481 gtcgacgcgc aaaaaagtta tttcattccg ccgccgcaga tgaaaaaagt catgcatggc
    21541 gaccgaatta tcgcggtgat ccacagtgaa aaagaacgtg aatccgcaga gccagaagaa
    21601 ctggttgaac cgttcctgac tcgtttcgtg ggtaaagttc agggcaaaaa tgaccgtctg
    21661 gccatcgttc ctgatcatcc actcttaaaa gacgccattc cttgccgcgc agcccgtggc
    21721 ctgaaccacg agtttaaaga aggcgactgg gcggttgccg aaatgcgccg tcatccgctg
    21781 aaaggcgatc gttctttcta tgctgaattg acacaataca tcacttttgg tgacgaccac
    21841 tttgtaccgt ggtgggttac acttgcgcgc cataatctgg aaaaagaagc accagacggc
    21901 gtcgctaccg aaatgctcga tgaaggtctg gttcgtgaag atctgaccgc gctggatttt
    21961 gtcaccatcg acagtgccag cacagaagat atggatgacg cccttttcgc taaggcgttg
    22021 ccggatgaca aacttcagct gattgtggcg attgccgatc caaccgcgtg gattgctgaa
    22081 ggcagcaagc tggacaaagc cgcgaaaatt cgcgcattca ccaactatct gcctggcttc
    22141 aacatcccta tgctgcctcg cgagctttct gacgatctct gctcactgcg cgccaatgaa
    22201 gtccgcccgg tactggcatg ccgcatgacg ctctccgctg atggcgccat tgaagataat
    22261 atcgaattct ttgccgccac catcgaatcc aaagcaaagc tggtgtatga ccaggtttct
    22321 gactggctgg aaaataccgg tgactggcag cctgaaagtg aagcaattgc cgaacaagtc
    22381 cgtttgctag cgcaaatttg ccaacgccgc ggcgagtggc gtcataacca cgcactggtg
    22441 tttaaagatc gcccggatta ccgctttatt ctcggtgaaa aaggtgaagt gctggatatc
    22501 gtcgccgagc ctcgtcgcat tgccaaccgt atcgtcgaag aagcgatgat tgccgctaac
    22561 atttgtgcgg cccgcgtact gcgcgataag ctcggttttg gcatctataa cgtgcatatg
    22621 ggctttgatc cggcgaatgc cgacgcgctg gcagcgttgc tgaaaacgca cggtctgcat
    22681 gtcgatgccg aagaagtgct cacgctggac ggtttctgca aactgcgtcg tgaactggac
    22741 gcgcaaccaa ctggtttcct cgacagccgc attcgtcgct tccagtcatt tgctgaaatt
    22801 agcactgaac ccggtcctca ctttggcctc ggtctggaag catacgccac ctggacttcg
    22861 ccgatccgta aatatggcga catgatcaac caccgtctgc tgaaagcggt tatcaaaggc
    22921 gaaactgcga cgcgcccaca ggatgaaatc actgtccaaa tggccgagcg tcgccgtctc
    22981 aaccggatgg cagaacgtga tgttggtgac tggttatacg cacgcttcct gaaagacaaa
    23041 gccgggaccg acacccgttt cgcagcggaa attgtcgata tcagccgtgg cggcatgcgt
    23101 gtgcgtttgg ttgataacgg cgctatcgcc tttattccgg cacctttctt acacgctatg
    23161 cgcgatgaac tggtttgcag ccaggaaaac ggcactgtac aaattaaagg tgaaacggtt
    23221 tacaaagtaa ctgacgttat tgacgtcacc attgccgaag tccgcatgga aacccgcagc
    23281 attattgcgc gcccggtcgc gtaatctcct ttcacggcct attcctcatg gatgggccgt
    23341 ttatttcccc gctctacctt cgttatatcc aggcagattt tttaactgac tttcgtttga
    23401 aaactggcgt ttttccaaaa ctggattaca aataaatata taaaaaaaca atgcgtttag
    23461 ccgccgttaa tttcatgtaa gtcatg
//
LOCUS       DAJVUJ010000052        23029 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.052, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000052 DAJVUJ010000000
VERSION     DAJVUJ010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23029
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.052"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(<1..561)
                     /locus_tag="OQQ48_001873"
     CDS             complement(<1..561)
                     /locus_tag="OQQ48_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCS1952669.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGG"
     gene            complement(780..1757)
                     /gene="epmA"
                     /locus_tag="OQQ48_001874"
     CDS             complement(780..1757)
                     /gene="epmA"
                     /locus_tag="OQQ48_001874"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HCS1952670.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLNLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            2082..3890
                     /gene="frdA"
                     /locus_tag="OQQ48_001875"
     CDS             2082..3890
                     /gene="frdA"
                     /locus_tag="OQQ48_001875"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HCS1952671.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRMAGEQATERAATAGNGNEEAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            3883..4617
                     /gene="frdB"
                     /locus_tag="OQQ48_001876"
     CDS             3883..4617
                     /gene="frdB"
                     /locus_tag="OQQ48_001876"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCS1952672.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4628..5023
                     /gene="frdC"
                     /locus_tag="OQQ48_001877"
     CDS             4628..5023
                     /gene="frdC"
                     /locus_tag="OQQ48_001877"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCS1952673.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPDAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            5034..5393
                     /gene="frdD"
                     /locus_tag="OQQ48_001878"
     CDS             5034..5393
                     /gene="frdD"
                     /locus_tag="OQQ48_001878"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCS1952674.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            5456..6589
                     /locus_tag="OQQ48_001879"
     CDS             5456..6589
                     /locus_tag="OQQ48_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCS1952675.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQMPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6678..7211
                     /gene="blc"
                     /locus_tag="OQQ48_001880"
     CDS             6678..7211
                     /gene="blc"
                     /locus_tag="OQQ48_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCS1952676.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDGGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGLFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(7208..7525)
                     /gene="sugE"
                     /locus_tag="OQQ48_001881"
     CDS             complement(7208..7525)
                     /gene="sugE"
                     /locus_tag="OQQ48_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCS1952677.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7707..7853)
                     /gene="ecnB"
                     /locus_tag="OQQ48_001882"
     CDS             complement(7707..7853)
                     /gene="ecnB"
                     /locus_tag="OQQ48_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCS1952678.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7964..8089)
                     /gene="ecnA"
                     /locus_tag="OQQ48_001883"
     CDS             complement(7964..8089)
                     /gene="ecnA"
                     /locus_tag="OQQ48_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCS1952679.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(8141..8707)
                     /gene="efp"
                     /locus_tag="OQQ48_001884"
     CDS             complement(8141..8707)
                     /gene="efp"
                     /locus_tag="OQQ48_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCS1952680.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8749..9798
                     /gene="epmB"
                     /locus_tag="OQQ48_001885"
     CDS             8749..9798
                     /gene="epmB"
                     /locus_tag="OQQ48_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HCS1952681.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEIDETFRQAMAKLRRVGVTLLNQSVLLRDVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQLLIKQGS"
     gene            10172..11041
                     /locus_tag="OQQ48_001886"
     CDS             10172..11041
                     /locus_tag="OQQ48_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HCS1952682.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQENGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEVEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(11244..11597)
                     /locus_tag="OQQ48_001887"
     CDS             complement(11244..11597)
                     /locus_tag="OQQ48_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HCS1952683.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(11735..13381)
                     /gene="groL"
                     /locus_tag="OQQ48_001888"
     CDS             complement(11735..13381)
                     /gene="groL"
                     /locus_tag="OQQ48_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCS1952684.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(13425..13718)
                     /locus_tag="OQQ48_001889"
     CDS             complement(13425..13718)
                     /locus_tag="OQQ48_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCS1952685.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            13994..15250
                     /gene="yjeH"
                     /locus_tag="OQQ48_001890"
     CDS             13994..15250
                     /gene="yjeH"
                     /locus_tag="OQQ48_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HCS1952686.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAAQVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPCAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRSASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEKMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIVLAGLWLLLPKRKTPENGIIT"
     gene            complement(15266..15742)
                     /gene="fxsA"
                     /locus_tag="OQQ48_001891"
     CDS             complement(15266..15742)
                     /gene="fxsA"
                     /locus_tag="OQQ48_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HCS1952687.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDERDRLDHKDDRQD"
     gene            16079..17515
                     /gene="aspA"
                     /locus_tag="OQQ48_001892"
     CDS             16079..17515
                     /gene="aspA"
                     /locus_tag="OQQ48_001892"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HCS1952688.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            17633..18934
                     /gene="dcuA"
                     /locus_tag="OQQ48_001893"
     CDS             17633..18934
                     /gene="dcuA"
                     /locus_tag="OQQ48_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HCS1952689.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            19050..19388
                     /gene="cutA"
                     /locus_tag="OQQ48_001894"
     CDS             19050..19388
                     /gene="cutA"
                     /locus_tag="OQQ48_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HCS1952690.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            19364..21061
                     /gene="dsbD"
                     /locus_tag="OQQ48_001895"
     CDS             19364..21061
                     /gene="dsbD"
                     /locus_tag="OQQ48_001895"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HCS1952691.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFTFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNHALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NITANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            21098..21673
                     /locus_tag="OQQ48_001896"
     CDS             21098..21673
                     /locus_tag="OQQ48_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCS1952692.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKQLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            21780..21855
                     /locus_tag="OQQ48_001897"
     tRNA            21780..21855
                     /locus_tag="OQQ48_001897"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21813..21815,aa:Phe,seq:gaa)
     gene            22053..>23029
                     /locus_tag="OQQ48_001898"
     CDS             22053..>23029
                     /locus_tag="OQQ48_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCS1952693.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGTGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGVDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIGS
                     YKGQTVTQLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQAVAILTELQTWAGENGLIFTGAHDPRKPISENVMNAS"
BASE COUNT         5738 a         5942 c         5699 g         5650 t
ORIGIN      
        1 cccaccattt aataacaaaa tagtaatgct tactaacagc aatactaaat tcagacacat
       61 tactgcaata ccgcccaccg cagtaatgcg ggcaatttta ttaatcccct ttgaagcaac
      121 gacggtgacc agaatcatcc atgctactgc cagcagacca accacctgcg taggctccag
      181 tccggcaata cgccagtgct gggtcatgtc gctaccatag aggaatgttg agaacggtac
      241 ccaaactttc gcggaggtac tcaccatcca gatgatataa gaggaaaacc acataaacgt
      301 accaatgaag gcaaaacgtg gtccgacact attattcatc caggaataga taccaccttc
      361 ttctttgcga taagcagctc ccatttcagc catcattaag gcgaatggaa taaagaataa
      421 caatgcagaa aatatataaa agggaatcgc actataaccc attaagtaat aagccgatgg
      481 gctattggca aatccaaata cggaagtaaa gatcatcaat atgagtccta tcagactcat
      541 cttttttatc gtgtgaggca tgaaaccatc cttcatttca gcgcgtgtat aacacgtgcc
      601 cactgaactg cactgcgcaa cattgagcag gcagcgtaaa aaccaataca gaattgatac
      661 ccggatgata tcaaatatcc agtcttaaat tgtggccttt aacggaaaaa tgaaaacata
      721 tgctacagaa tggcgcttat cacgccattc ttcgctgtta attcagtaat ttttcagaat
      781 tatgcccggt caacgctaaa ggcgatgact tcagccagtg tctccgcgcc cagcgccaac
      841 atcaccagac gatcaacacc taatgccacg ccggaacagt caggcatacc gactttcaag
      901 gcttcaatca gattctggtc aatggggtgc tgcggcaaac cgcgcgccgc gcgcttacgg
      961 ttatcttgtt caaagcgttg ttgctgctca cgggcatccg ttaattcatg gaaaccattc
     1021 gccagctcaa tacctttata ataaacctca aagcgttcag cgacccgatg atcttcggta
     1081 ctgatttgcg ccagtgatgc ctggctggct ggaaagtggt acacaaaggt cggtttttct
     1141 ttgccaatat ttggctctac gccaaaggta aacagcaatt gtagcaacgt gtcgcggtct
     1201 tcttcggtat cagcaacgtt actcaaattc agtttcgctg cgacttcccg cagttgcgtt
     1261 ttgtcggcag agagcgggtc aatttccaga taacgcaaga aagcttgttg ataagaaagg
     1321 ctttctgctg ccgggcagtc cagcacctgt tgtaagagat cgtccacctc gttcatcaac
     1381 cggtacatat catagtgcgg tcgataccac tcaagcatag tgaactcagg gttgtgataa
     1441 cgccccatct cttcattacg gaagctgcgg cacagctgga ataccggccc acaaccggca
     1501 accagcaggc gtttcatatg gtattccggg ctggtcatta accagagatt catcccctgc
     1561 gaatgcccgg ggccaacgaa acgtgtctca aacgggacca aatgaatatc ggttaccgtc
     1621 gcctggctca tacaaggcgt ctccacctcc agcactccac gatcagcaaa gaaacgacgg
     1681 atctccgcca taatcgccgc gcgttttaat aagttaggaa tggatgcgct cggctgccag
     1741 gatgccgttt cgctcatagt taaatctcca gtttttgaca agggcacgaa gtctactcgc
     1801 aacgcgacgg cgagacaaat tttacgcagg aatcaaacag cggtgggcag tgactaaaaa
     1861 aagcacgatc tgatggttta gtaattaaat taatcatctt cagtgataat ttagccctct
     1921 tgcgcactaa aaaaatcgat ctcgtcaaat ttcagactta tccatcagac tatactgttg
     1981 tacctataaa ggagcagtgg aatagcgttc gcagaccgta actttcaggc acttaccctg
     2041 aagtacgggg ctgtgggata aaaacaatct ggaggaatgt cgtgcaaacc tttcaagccg
     2101 atcttgccat tgtaggcgcc ggtggcgcgg gattacgtgc tgcaattgct gccgcgcagg
     2161 caaatccaaa tgcaaaaatc gcactaatct caaaagtata cccgatgcgt agccataccg
     2221 ttgctgcaga agggggctcc gccgctgtcg cgcaggatca tgacagcttc gaatatcact
     2281 ttcacgatac agtagcgggt ggcgactggt tgtgtgagca ggatgtcgtg gattatttcg
     2341 tccaccactg cccaaccgaa atgacccaac tggaactgtg ggggtgccca tggagccgtc
     2401 gcccggatgg tagcgtcaac gtacgtcgct tcggcggcat gaaaatcgag cgcacctggt
     2461 tcgccgccga taagaccggc ttccatatgc tgcacacgct gttccagacc tctctgcaat
     2521 tcccgcagat ccagcgtttt gacgaacatt tcgtgctgga tattctggtt gatgatggtc
     2581 atgttcgcgg cctggtagca atgaacatga tggaaggcac gctggtgcag atccgtgcta
     2641 acgcggtcgt tatggctact ggcggtgcgg gtcgcgttta tcgttacaac accaacggcg
     2701 gcatcgttac cggtgacggt atgggtatgg cgctaagcca cggcgttccg ctgcgtgaca
     2761 tggaattcgt tcagtatcac ccaaccggtc tgccaggttc cggtatcctg atgaccgaag
     2821 gctgccgtgg tgaaggcggt attctggtca acaaaaatgg ctaccgttat ctgcaagatt
     2881 acggcatggg cccggaaact ccgctgggcg agccgaaaaa caaatatatg gaactgggtc
     2941 cacgcgacaa agtttctcag gccttctggc acgaatggcg taaaggcaac accatctcca
     3001 cgccgcgtgg cgatgtggtt tatctcgact tgcgtcacct cggcgagaaa aaactgcatg
     3061 aacgtctgcc gttcatctgc gaactggcga aagcgtacgt tggcgtcgat ccggttaaag
     3121 aaccgattcc ggtacgtccg accgcacact acaccatggg cggtatcgaa accgatcaga
     3181 actgtgaaac ccgcattaaa ggtctgttcg ccgtgggtga atgttcctct gttggtctgc
     3241 acggtgcaaa ccgtctgggc tccaactccc tggcggaact ggtggtcttc ggccgtatgg
     3301 ccggtgaaca agcgacagag cgtgcagcaa ctgccggtaa tggcaacgaa gaggcaattg
     3361 aagcgcaggc agctggcgtt gaacaacgtc tgaaagatct ggttaaccag gatggcggcg
     3421 aaaactgggc gaagatccgc gacgaaatgg gcctagcaat ggaagaaggt tgcggtatct
     3481 accgtacgcc ggaactgatg cagaaaacca tcgacaagct ggcagagctg caggaacgct
     3541 tcaagcgcgt gcgcatcacc gacacctcca gcgtgttcaa caccgacctg ctctacacca
     3601 ttgaactggg ccacggtctg aacgttgctg aatgtatggc gcactccgca atggcacgta
     3661 aagagtcccg cggcgcacac cagcgtctgg acgaaggttg caccgagcgt gacgacgtca
     3721 acttcctcaa acacaccctc gccttccgcg atgctgatgg cacgactcgc ctggagtaca
     3781 gcgacgtgaa gattactacg ctgccgccag ctaaacgcgt ttacggtggc gaagcggatg
     3841 cagccgataa ggcggaagca gccaataaga aggagaaggc gaatggctga gatgaaaaac
     3901 ctgaaaattg aggtggtgcg ctataacccg gaagtcgata ccgcaccgca tagcgcattc
     3961 tatgaagtgc cttatgacgc aactacctca ttactggatg cgctgggcta catcaaagac
     4021 aacctggcac cggacctgag ttaccgctgg tcctgccgta tggcgatttg tggttcctgc
     4081 ggcatgatgg ttaacaacgt gccaaaactg gcatgtaaaa ccttcctgcg tgattacacc
     4141 gacggtatga aggttgaagc gttagctaac ttcccgattg aacgcgatct ggtggtcgat
     4201 atgacccact tcatcgaaag tctggaagcg atcaaaccgt acatcatcgg caactcccgc
     4261 accgcggatc agggtactaa catccagacc ccggcgcaga tggcaaagta tcaccagttc
     4321 tccggttgca tcaactgtgg tctgtgctac gccgcgtgcc cgcagtttgg cctgaaccca
     4381 gagttcatcg gtccggctgc cattacgctg gcgcatcgtt ataacgaaga tagccgcgac
     4441 cacggtaaga aggagcgtat ggcgcagttg aacagccaga acggcgtatg gagctgtact
     4501 ttcgtgggct attgctccga agtctgcccg aaacacgtcg atccggctgc ggccattcag
     4561 cagggcaaag tagaaagttc gaaagacttt cttatcgcga ccctgaaacc acgctaagga
     4621 gtgcaacatg acgactaaac gtaaaccgta tgtacggcca atgacgtcca cctggtggaa
     4681 aaaattgccg ttttatcgct tttacatgct gcgcgaaggc acggcggttc cggctgtgtg
     4741 gttcagcatt gaactgattt tcgggctgtt tgccctgaaa aatggcccgg acgcctgggc
     4801 gggattcgtc gactttttac aaaacccggt tatcgtgatc attaacctga tcactctggc
     4861 ggcagctctg ctgcacacca aaacctggtt tgaactggca ccgaaagcgg ccaatatcat
     4921 tgtaaaagac gaaaaaatgg gaccagagcc aattatcaaa agtctctggg cggtaactgt
     4981 ggttgccacc atcgtaatcc tgtttgttgc cctgtactgg taaggagcct gagatgatta
     5041 atccaaatcc aaagcgttct gacgaaccgg tattctgggg cctcttcggg gccggtggta
     5101 tgtggagcgc aattattgcg ccggtgatga tcctgctggt gggtattctg ctgccactgg
     5161 ggctgtttcc gggtgatgcg ctgagctacg agcgcgttct ggcgttcgcg cagagcttca
     5221 ttggtcgcgt attcctgttc ctgatgatcg ttctgccgct gtggtgtggt ttacaccgta
     5281 tgcaccacgc gatgcacgat ctgaaaattc acgtgcctgc gggcaaatgg gttttctacg
     5341 gtctggctgc tatcctgaca gttgtcacgc tgattggtat cgttacaatc taacgtatcg
     5401 ccaatgtaaa tccggcccgc ctatggcggg ccgttttgta tggaaaccag accttatgtt
     5461 caaaacgacg ctctgcgcct tattaattac cgcctcttgc tccacatttg ctgcccctca
     5521 acaaatcaac gatattgtgc atcgcacaat taccccgctt atagagcaac aaaagatccc
     5581 cggtatggcg gtggcggtaa tttatcaggg taaaccttat tactttacct ggggctatgc
     5641 ggacatcgcc aaaaagcagc ccgtcacaca gcaaacgttg tttgagttag gttcggtcag
     5701 caaaacattt acgggcgtgc ttggtggcga tgctattgct cgaggggaaa tcaagttaag
     5761 cgatcccgca acaaaatact ggcctgaact taccgctaaa cagtggaatg ggatcacact
     5821 attacatctt gcgacctaca ccgctggcgg cctgccattg cagatgccgg atgaagtgaa
     5881 atcctcaagc gacttgctgc gcttctatca aaactggcag cctgcatggg ctccaggaac
     5941 acaacgtctg tatgccaact ccagtatcgg tttgttcggc gcactggctg tgaagccgtc
     6001 tggtttgagt tttgagcagg cgatgcaaac tcgtgtcttc cagccactca aactcaacca
     6061 tacgtggatt aatgtaccgc ccgcagaaga aaagaattac gcctggggat atcgcgaagg
     6121 caaggcagtt catgtttcgc caggggcgtt agatgctgaa gcttatggtg tgaagtcgac
     6181 cattgaagat atggcccgct gggtgcaaag caatttaacc ccccttgata tcaatgagaa
     6241 aacgcttcaa caagggatac aactggcaca atctcgctac tggcaaaccg gcgatatgta
     6301 tcagggcctg ggctgggaaa tgctggactg gccggtcaat cctgacagca tcattaacgg
     6361 cagtgacaat aaaattgcac tggcagcacg cccggtaaaa gcgattaagc ccccaactcc
     6421 tgcagtacgc gcatcatggg tacataaaac gggggcgacc ggcggatttg gtagctatgt
     6481 cgcgtttatt ccagaaaaag agctgggtat cgtgatgctg gctaacaaaa actatcccaa
     6541 tccagcgaga gtcgccgccg cctgtcagat tctcaacgct ctacagtaaa atttcatcgg
     6601 gtccgaattt tcggaccttt tctgcgcttt tccttgctgt catctacact tagaaaaaaa
     6661 ccagtaagga aacatttatg cgcctgctcc ctctcgttgc cgcagcgaca gctgcatttc
     6721 tggtcgttgc ctgcagttct cctacgccgc cgcgtggcgt gacagtagtg aataatttcg
     6781 acgctaaacg ctatctcggt acctggtatg agattgcccg ttttgatcac cgttttgaac
     6841 gtggactgga aaaagtcacc gcaacataca gtctgcgtga tggcggcggc ctgaatgtca
     6901 tcaataaagg ctataaccct gacagagaaa tgtggcagca gagtgaaggg aaagcgtact
     6961 ttaccggcgc accaacacgc gctgcgctga aagtgtcatt ctttggtctt ttctatggcg
     7021 gttataacgt cattgcactt gatcgggaat accgccatgc gctggtttgc ggcccggacc
     7081 gcgactacct gtggatactc tcccgcacgc caaccatttc tgacgaagtg aaacaggaga
     7141 tgctggcagt cgcgacccgg gaagggtttg atgtcagtaa gtttatttgg gtacagcagc
     7201 ccggtagtta gtgagtgctg agtttcagac caataatccc caatacgatt aacgccagac
     7261 tcgccaggcg catcgggtta gcggactcac cgagcagcac aatgccggtg atagccgcgc
     7321 cgactgcgcc aatacccgtc cacacggcat aagccgtccc tactggtaac gatttcatcg
     7381 cccaggcaag tagcgccata ctgacaatca tcgccgtcac ggtaataaca ctcggcgtca
     7441 aacgactaaa gccgtgggta tatttcaggc caacggccca taccacttcc agcagaccag
     7501 caataactaa gataatccag gacatatcag gctccggaga attggggccg tccccggtga
     7561 aaagacgcgt ttgcaggtcg tcctgcaaag ctgatggttg aacggatatt ttgccaggtg
     7621 gcaggggaat ttcaactatt accttagtga ctatcgcaga aaaaacaaac ggcacgacac
     7681 agctgtcgta ccgttattgc ttattgctat tgctgcgctt tcgttgctgc gccagaaatc
     7741 gcgttaccgc catcagaaat gtcttcacca acgccacgcg tggtgttgca ggcagttaat
     7801 actgttgaaa gcaccagaac agaaaagatc gctgcaattg tcttcttcac cataacgtct
     7861 tccttttagg taatgttatt tatgtttgcc tatagcaaat taataataga caacatcgcc
     7921 aaaaatgacg gaaatctgat gatttttcgg aagagaagaa aaattagctg gcagcgcgag
     7981 agatggagtt gccgagatgt ttgatgtctt cgccgaaacc gcgagcggtg ttacagcccg
     8041 tgagcagcgt actggcaagc aaaacaagaa cgataaggcg tttcatcatc tctgacctga
     8101 ataagtgatg gtgcagcctg caggccgcac cacaaccgca ttacttcacg cgagagacgt
     8161 attcaccaga gcgggtatcc actttgatga cttcgccgat ttgtacgaac agcggaacct
     8221 taaccacagc gccagtagac agggtagccg gtttgccgcc agtacctgcg gtatcacctt
     8281 tcaggcccgg atcggtatca acgatttcca gttcaacgaa gttcggcgga gtaacggaga
     8341 tcggctgacc attccacaga gttacgatac actctgcctg atccagcagc catttagcgt
     8401 tatcgccaat tgcttttgca tcagcagaca gctgctcgaa agtttcgttg ttcatgaagt
     8461 gccagaactc accgtcgttg tacaggtaag tcaggttcat atcgacaaca tcagcgcctt
     8521 cagcggaatc agtagatttg aaggtttttt ctacgcgagt accggtcagc agacgacgca
     8581 gtttaacgcg agcaaatgcc tggcctttac ccggttttac gaattcactc gcttcaaccg
     8641 cgtaaggttc gccgtctaac atgattttaa gaccagcacg aaaatcgttg ctatagtacg
     8701 ttgccataag gccctctgaa atttgttaat tggtagctaa gccacaaaat ggcgcatatt
     8761 gtaaccctaa ataccccatc cagagaagat tggttaacgc aacttgccga tgttgtgacc
     8821 gatcctgatg aacttctgcg tcttttgaat atagacgcgg acgaaaaact gttggccgga
     8881 cgcagcgcca aaaagctgtt tgccctgcgt gtgccccgct catttatcga tcgcatggag
     8941 aaaggcaatc cggacgatcc tcttttgcgt caggtactta cctcgcaaga tgagtttgtc
     9001 gtcgcgtccg gattctccac cgacccactg gaagaacagc acagcgtagt gcctggtttg
     9061 ttgcataaat accacaaccg ggcgcttttg ctggtcaaag gcggctgcgc ggtaaattgc
     9121 cgctattgct tccgtcgcca cttcccctat gccgaaaatc agggcaacaa gcgtaactgg
     9181 cagactgcac ttgagtatgt tgctgcgcat ccggaactgg acgagatgat tttctccggc
     9241 ggcgatccgc tgatggcgaa agatcacgag ctggactggt tgctcacaca actggaagcc
     9301 atcccgcata tcaaacgtct gcggattcac agtcgtctgc cgattgtgat cccggcacgc
     9361 atcacagatg cgctggttga acgcttttca cattctacgc tgcaaatcct gctggtgaat
     9421 cacatcaacc atgccaatga gatagatgaa acattccgtc aggcgatggc taagttgcgc
     9481 cgggtaggcg ttactttgct gaaccagagc gttctgttac gtgatgtgaa cgataacgca
     9541 caaacgctgg caaacctgag taatgcgttg ttcgatgccg gcgtgatgcc gtattacctg
     9601 catgtgctcg ataaagtaca gggcgcagcg cattttatgg tgagtgatga cgaagcacgg
     9661 cagattatgc gtgagttgct gacattggtg tcgggatatc tggtgccgaa actggcgcga
     9721 gaaattggcg gcgaacccag caaaacgccg ctggatctcc agctacgcca gcagttactg
     9781 attaaacagg gaagttaaac attaaaattg cctgatgcgc tacgcttatc aggcctacat
     9841 cgtgcattga aattgaatgg atttgcaatg aactgtaggc cggataaggc gtttacgccg
     9901 catccggcaa gagcaacaaa cgcaagtaca gcaacctgtc attaaactga gaaatttaat
     9961 ctttgcccgt tgcataacct ttaattattc atatgcattg ctgttgttac tttttatata
    10021 gctaacacca ataattaacc tttcttttct gtttatatag agtgagcacg gctatttatg
    10081 ttattttaac aactgttact accaagccca tttatctggc ttataaatta aaattaatta
    10141 tttaattgag aaatggttag ggagaaccta catggccata agcatcaagg gtgttaatac
    10201 cggtgttatt cgcaagagta ataatttcat tgcactggcg ctgaaaatta aagaacctcg
    10261 caataaagaa tcgttgttct ttatgtctgt catggaactg cgcgacctgc tgattgcgct
    10321 ggaaagtcgt ctgcatcaaa agcataaact ggatgctgct gcacgccttc agtatgagca
    10381 agctcgcgat aaagtcatta agaaaatggc agaaaacatc ccagaaattc tggttgatga
    10441 gcttaaaaat gccgatatta atcgtcgggt taacacgctg gaacttaccg ataatcaagg
    10501 cgaaaatctt accttcgtat taacgctaca tgacggtagc aaatgtgagt tagtcgtcaa
    10561 tgagttgcag attgaaatgc tagcgcgagc aatcattcat gcaattaata atgcggaaat
    10621 gcgtgaactg gcattacgta ttacctcact gttagatttc ctgccgttat atgacgttga
    10681 ctgccaggaa aatggcaatc tggaatatga cacctattcc caaccggaat ggaaacataa
    10741 cttgttcgac cattatctgg cggtgcttta ccgctttaaa gatgaaagtg gcaaagaaca
    10801 atttagcggc gcagtggtga aaacgcgtga agcaacgccg ggtaaagaag tagaggcgat
    10861 cactcgccga atgcttgatt tcagcccacg actgaaaaaa ctcgcgggtg tgccatgtca
    10921 ggtctatgtt cgcaccgtag cggcgaataa cgcccaaccg ctaacccagg atcaatgctt
    10981 acgcgcgctg catcacttac gtgttcaatc caccagcaaa actgcaccac aggcgaaata
    11041 atctcatctt tgctaatgcg aggctggagc catccagcct gaaatgactg ataacgtgat
    11101 cgttggtcat gccggatgcg gcgtaaacgc cttatccggc ctacaaattc tcaagcattc
    11161 aatcagttgc aatgcctgct gtaggcctga taagcgaagc gcatcaggca ataatacgtt
    11221 tgtcacccag cctctggatc acatcagttc gggcacttat aaacctgacc gataatctgg
    11281 ctatccgtcg ggacaaaact ggacaacatt ccctgcgacg ggctgctgat gccataaatc
    11341 acgttaccgc ccattgcagc cgcctggttg cgcagatcgt ttgctgcgcc gcgcatagaa
    11401 ccgccctctt ctccgtgttg cccggaaagc cagttgcttt gcttacctgt cgcagtacca
    11461 atcagctggc actctgcgcc tggctgctcg tccacaatgc gtacactctg accggcagca
    11521 ctcaattcgt tgctggagct acaacccgcc atcagtagcg cggcaccgac aatccctgct
    11581 aagtatttta cgtgcatgtt attccccata atcaatgagc tggacgccac tcgcgtcgtc
    11641 cgtgtctgaa tcttatacta gaaagatgac caaaagaaaa acccccagac atttctgccc
    11701 gggggtttgt ttatttctgc gaggtgcagg gcaattacat catgccgccc atgccaccca
    11761 tgccgcccat accgccagca gcgcctaagt cagctgcatc gtttttcggc aggtcggtaa
    11821 ccatgcactc ggtggtgatc atcaggccag ccacagaagc cgcgtactgc agagcagaac
    11881 gggttacttt ggttggatcc aagataccca tgtcgatcat gttgccgtat tcttcggttg
    11941 ctgcgttgta accgtagttg ccgtcgccgc ctttaacagt gttagcaaca acagacggtt
    12001 cttcgccgca gttcaatacg atctgacgca gcggagcttc cattgcacgc agtgcaactt
    12061 tgatacccac gttctggtct tcgttctgac cacgcaggtc agccagttta gacgctacgc
    12121 ggatcagcgc aacaccacca ccagcaacca cgccttcttc taccgcagca cgggtcgcgt
    12181 gcagggcatc ttcaacgcgt gcttttttct ctttcatttc aacttcggta gcagcaccta
    12241 ctttgataac tgcaacgccg cctgccagtt tcgctacgcg ctcctgcagt ttttcacggt
    12301 cgtagtcaga agttgcttct tcaatctgct gacggatctg agcaacacgg ccctggattg
    12361 cagcttcttc acccacgcca tcaatgatgg tggtggtgtc tttgttgatc acaacacgtt
    12421 tagcctgacc caggtcttcc agggttgctt tttccagctc cataccgatc tcttcagaga
    12481 ttacggtacc gccagtcagg gttgcgatat cctgcagcat agctttacgg cgatcgccga
    12541 agcccggagc tttaaccgca gcaactttca cgatgccacg catggtgtta acaaccagag
    12601 ttgccagcgc ttcgccttca acatcttcag cgatgatcag cagcggttta cctgctttag
    12661 caacggcttc cagaaccggc agcatttcgc ggatgttgga gattttcttg tcagccagca
    12721 ggatgaacgg gctttccagt tctactgcgc cagtttccgg cttgttgatg aagtaaggag
    12781 acaggtagcc acggtcgaac tgcatacctt caaccacgtc cagttcgtcc tgcagaccgg
    12841 taccgtcttc aacggtgata acgccttctt taccgacttt gtccatcgct tcagcgatca
    12901 gtttacctac ggtttcgtcg gagttagcgg agatggtacc aacctgagca atcgctttag
    12961 agtcagagca tggtacggac agcgctttca gttcttcaac tgcagcggta acagctttgt
    13021 cgataccacg tttcaggtcc atcgggttca tgcccgcagc aactgctttc agaccctcag
    13081 tgatgatagc ctgagccagt acggttgcag tggtggtacc gtcgcctgca gcgtcgttcg
    13141 ctttagaggc aacttctttc accatctgcg cacccatatt ttcgaacttg tcttccagtt
    13201 cgatttcacg agcaacggaa acaccatctt tggtgatggt cggtgcaccg aaagatttat
    13261 ccagaactac gttacggcct ttcggaccga gggtaacttt cactgcatct gccagtacgt
    13321 ttacgccgcg cagcattttc acacgagcgt cgttaccgaa ttttacgtct ttagctgcca
    13381 ttatctttat tccttaaatt cgtatgttca gtgtcgtgcg aggattacgc ttcaacaatt
    13441 gccagaatgt cgctttcgga catgatcaac acttcttcat tgtcgatctt ctcagatttc
    13501 acaccgtagc catcgttgaa aataacgatg tcgccaactt tcacatccag cggcttcact
    13561 tcgccatttt caaggatacg gccattgccg acagccagca cttcgccgcg ggtggattta
    13621 gccgctgcag agccggtcag aacgatgccg ccagcagatt tagtttcaac ttctttacgc
    13681 ttgacgatca cgcgatcatg caatggacga atattcattg ataactctcc tttgagaaag
    13741 tccgtatctg ttatgggtga cgccggagct tacgtggttt cccggctggt gaccagagaa
    13801 atggggatga ggattcgccc cttcaagggg gaaaaagaaa aaaaattctg atcagcacaa
    13861 aatcgggtga aaaccctgat tcacctcaca tttcatcgca atttcttcat cgccgtcata
    13921 agcgaatctg attgtgctac catcgaaaat ctacgcagtt gcccaaaatt tgggcgcaat
    13981 cggatatcaa cccatgagtg gactcaaaca agaactgggg ctggcccagg gcattggcct
    14041 gctatcgacg tcattattag gcaccggcgt gtttgccgtt cctgcgttag ctgcacaggt
    14101 agcaggcaat aacagcctgt gggcgtggcc cgttttgatt atcttagtgt tcccgattgc
    14161 gattgtgttt gcgattctgg gtcgccacta tccctgcgca ggcggtgtcg cgcacttcgt
    14221 cggtatggcg tttggttcgc ggcttgagcg agtcaccggc tggttgtttt tatcggtcat
    14281 tcccgtgggt ttgccagccg cgctacaaat tgccgccggg ttcggccagg caatgtttgg
    14341 ctggcatagc tggcaactgt tgttggcaga actcggtacg ctggcgctgg tgtggtatat
    14401 cggtactcgc agtgccagtt ccagtgctaa tctacaaacc gttattgccg gacttatcgt
    14461 cgcgctgatt gtcgctatct ggtgggcggg cgatatcaaa cctgcgaata tccccttccc
    14521 ggcacctggt aatatcgaac ttaccgggtt atttgctgcg ttatcagtga tgttctggtg
    14581 ttttgtcggt ctggaagcat ttgcccatct tgcctcggaa tttaaaaatc cagagcgtga
    14641 ttttcctcgc gcattgatga ttggtctgct gctggcagga ttagtctact ggggctgtac
    14701 ggtagtcgtc ttacacttcg acgcctatgg tgaaaaaatg gcggcggcag catcgcttcc
    14761 aaaaattgta gtgcaactgt tcggtgtagg agcgttatgg attgcctgcg tgattggcta
    14821 tctggcctgc tttgccagtc tcaacattta tatacagagc ttcgcccgcc tggtctggtc
    14881 gcaggcgcaa cataatcctg accactacct ggcacgcctc tcttctcgcc atatcccgaa
    14941 taatgccctc aatgcggtgc tcggctgctg tgtggtgagc actttggtga ttcatgcttt
    15001 agagatcaat ctggacgctc ttattattta tgccaatggc atctttatta tgatttatct
    15061 gttatgcatg ctggcaggct gtaaattatt gcaaggacgt tatcgactac tggcggtggt
    15121 tggcgggctg ttatgcgttc tgttactggc aatggtcggc tggaaaagtc tctatgcgct
    15181 gatcgtgctg gcggggttat ggctgttgct gccaaaacga aaaacgccgg aaaatggcat
    15241 aatcacataa tccggcgttt cgacattaat cctggcgatc gtctttatga tcaaggcggt
    15301 cgcgctcatc atcctttcgc tggtactcac catcaaaagt attaccgcca ccggtcccgg
    15361 cgctaaaacc gccgcctggc atacgagaaa agcgcaaatg cggcatcaac ttcactgtca
    15421 gatgcttttg caccggcggt aataaaagca gaagaccgag gaagtcggtg aaaaagcccg
    15481 gtaataaaag cagcaaaccg gcaatgatca gcgaaacact tttaatcatc tccgccgctg
    15541 ggttttcacc cgccgccatt ttttgctgca tcagcacgaa attcttaaag ccctggttac
    15601 gtaccagtga catcccgata actgacgtga atataaccag cacgagggtc agcaataccc
    15661 ccaatacatg ggcaacctga ataaagattg aaatctcaat atagacataa aggaaaatgg
    15721 caataaaagg taaccagcgc aaaggtttct cctgtaatag cagccggtaa aacccggcta
    15781 cctgaatggg ttgcgaatcg cgttcagctt atattgtggt cattagcaaa atttcaagat
    15841 gtttgcgcaa ctatttttgg tagtaatccc aaagcggtga tctatttcac aaattaataa
    15901 ttaaggggta aaaaccgaca cttaaagtga tccagattac ggtagaaatc ctcaagcagc
    15961 atatgatctc gggtattcgg tcgatgcagg ggataatcgt cggtcgaaaa acattcgaaa
    16021 ccacatatat tctgtgtgtt taaagcaaat cattggcagc ttgaaaaaga aggttcacat
    16081 gtcaaacaac attcgtatcg aagaagatct gttgggtacc agggaagttc cagctgatgc
    16141 ctactatggt gttcacactc tgagagcgat tgaaaacttc tatatcagca acaacaaaat
    16201 cagtgatatt cctgaatttg ttcgcggtat ggtaatggtt aaaaaagccg cagctatggc
    16261 aaacaaagag ctgcaaacca ttcctaaaag tgtagcgaat gccatcattg ccgcatgtga
    16321 tgaagtcctg aacaacggaa aatgcatgga tcagttcccg gtagacgtct accagggcgg
    16381 cgcaggtact tccgtaaaca tgaacaccaa cgaagtgctg gccaatatcg gtctggaact
    16441 gatgggtcac cagaaaggtg aatatcagta cctgaacccg aacgaccatg ttaacaaatg
    16501 tcagtccact aacgacgcct acccgaccgg tttccgtatc gcagtttact cttccctgat
    16561 taagctggta gatgcgatta accaactgcg tgaaggcttt gaacgtaaag ctgtcgaatt
    16621 ccaggacatc ctgaaaatgg gtcgtaccca gctgcaggac gcagtaccga tgaccctcgg
    16681 tcaggaattc cgcgctttca gcatcctgct gaaagaagaa gtgaaaaata tccaacgtac
    16741 cgctgaactg ctgctggaag ttaaccttgg cgcaacagca atcggtactg gtctgaacac
    16801 gccgaaagag tactctccgc tggcagtgaa aaaactggct gaagtcactg gcttcccatg
    16861 cgtaccggct gaagacctga tcgaagcgac ctctgactgc ggcgcttatg ttatggttca
    16921 cggcgcgctg aaacgcctgg ctgtgaagat gtccaaaatc tgtaacgacc tgcgcttgct
    16981 ctcttctggc ccacgtgccg gcctgaacga gatcaacctg ccggaactgc aggcgggctc
    17041 ttccatcatg ccagctaaag taaacccggt tgttccggaa gtggttaacc aggtatgctt
    17101 caaagtcatc ggtaacgaca ccactgttac catggcagca gaagcaggtc agctgcagtt
    17161 gaacgttatg gagccggtca ttggccaggc catgttcgaa tccgttcaca ttctgaccaa
    17221 cgcttgctac aacctgctgg aaaaatgcat taacggcatc actgctaaca aagaagtgtg
    17281 cgaaggttac gtttacaact ctatcggtat cgttacttac ctgaacccgt tcatcggtca
    17341 ccacaacggt gacatcgtgg gtaaaatctg tgccgaaacc ggtaagagtg tacgtgaagt
    17401 cgttctggaa cgcggtctgt tgactgaagc ggaacttgac gatattttct ccgtacagaa
    17461 tctgatgcac ccggcttaca aagcaaaacg ctatactgat gaaagcgaac agtaatcgta
    17521 cagggtagta caaataaaga aggcacgtca gatgacgtgc cttttttctt gtgaacagta
    17581 acttaaaaac aacaacctaa tatcaacttg ttaaaaaaca aggaaggcta atatgctagt
    17641 tgtagaactc atcatagttt tgctggcgat cttcttgggc gccagattgg ggggaatagg
    17701 tattggtttt gcaggcggat tgggggtgct ggttcttgcc gctattggcg ttaaacccgg
    17761 taacatcccg ttcgatgtca tttccattat catggcggtt atcgccgcta tttctgccat
    17821 gcaggttgct ggcggtctgg actatctggt tcatcagaca gaaaagctgc tgcgccgtaa
    17881 cccgaaatac atcacgatcc tcgcacctat cgtgacctat ttcctgacaa tctttgctgg
    17941 tactggcaac atctctctgg cgacactgcc agttatcgct gaagttgcga aggaacaagg
    18001 cgttaaacct tgccgtccgc tctctactgc agtggtatcc gcgcagattg cgatcaccgc
    18061 atcgccaatc tcagcggcag tggtttacat gtcttccgtg atggaaggtc atggcatcag
    18121 ctacctccat ctgctctccg tggtcatccc gtccaccctg ctggcggttc tggtgatgtc
    18181 ctttctggtc actatgctgt tcaactccaa actctctgac gatccgattt atcgcaagcg
    18241 tctggaagag ggcctggttg aactgcgcgg tgaaaagcag attgaaatca aatccggtgc
    18301 aaaaacgtcc gtctggctgt tcctgctggg cgtagttggc gtggttatct atgcaatcat
    18361 caacagccca agcatgggtc tggttgaaaa accgctgatg aacaccacca acgcaatcct
    18421 gatcatcatg ctcagcgttg caactctgac caccgttatc tgtaaagtcg ataccgacaa
    18481 catcctcaac tccagcacct tcaaagcagg tatgagcgcc tgtatttgta tcctgggtgt
    18541 tgcgtggctg ggcgatactt tcgtttccaa caacatcgac tggatcaaag ataccgctgg
    18601 tgaagtgatt cagggtcatc cgtggctgct ggccgtcatc ttcttctttg cttctgctct
    18661 gctgtactct caggctgcaa ccgcaaaagc actgatgccg atggctctgg cactgaacgt
    18721 ttcaccgctg accgctgttg cttctttcgc tgcggtgtct ggtctgttca ttctgccgac
    18781 ctacccgacg ctggttgctg cggtacagat ggatgacacg ggtactaccc gtatcggtaa
    18841 attcgtcttc aaccatccgt tcttcatccc gggtactctg ggtgttgccc tggccgtttg
    18901 cttcggcttc gtgctgggta gcttcatgct gtaatgatca atcgcggggc gttcacgccc
    18961 cgctttcttt cccgccgact aacatccttc ccccgtacgt tgtatagtga cctctctctt
    19021 gcggttccat ctgttcttgc gaggtgttta tgcttgatga aaaaagttcg aataccgcgt
    19081 ctgtcgtggt gctatgtacg gcaccagatg aagcgacagc ccaggattta gctgccaaag
    19141 tgctggcgga aaaactggcg gcctgcgcga ccttgatccc cggcgctacc tctctctatt
    19201 actgggaagg taagctggag caagaatacg aagtgcagat gattttaaaa actaccgtat
    19261 ctcaccagca ggcactgctg gaatgcctga agtctcatca tccatatcaa accccggaac
    19321 ttctggtttt acctgttaca cacggagaca cagattacct ctcatggctc aacgcatctt
    19381 tacgctgatc ctgctacttt gcagcacttc cgtttttgcc ggattattcg acgcgccggg
    19441 acgttcacaa tttgtccccg cggatcaagc ctttactttt gattttcagc aaaaccaaca
    19501 tgaccttaat ctgacctggc agatcaaaga cggttactac ctctaccgta aacagatccg
    19561 cattacgccg gaacacgcga aaattgccga cgtgcagctg ccgcaaggcg tctggcatga
    19621 agatgagttt tacggcaaaa gcgagattta ccgcgatcgg ctgacgcttc ccgtaaccat
    19681 caaccaggcg agtgcgggag cgacgttaac tgtcacctac cagggctgtg ctgatgccgg
    19741 tttctgttat ccgccagaaa ccaaaaccgt tccgttaagc gaagtggtcg ccaacaacgc
    19801 agcgtcacag cctgtgtctg tttcgcagca agagcagcac accgcgcaat tgcccttttc
    19861 cgcgctctgg gcgttgttga tcggtattgg tatcgccttt acgccatgcg tgctgccaat
    19921 gtacccactg atttctggca tcgtgctggg tggtaaacag cggctctcca ctgccagagc
    19981 attgttgctg acctttattt atgtgcaggg gatggcactg acttacacgg cgctgggtct
    20041 ggtagttgcc gccgcagggt tacagttcca ggcggcgcta cagcacccat acgtgctcat
    20101 tggcctcgcc atcgtcttta ccttgctggc gatgtcaatg tttggcttgt ttactctaca
    20161 actcccctct tcgctgcaaa cacgtctcac gttgatgagc aatcgccaac agggcggctc
    20221 acctggcggt gtgtttgtta tgggggcgat tgccggactt atctgttcac catgcaccac
    20281 cgcaccgctt agcgcgattc tgctgtatat cgcccaaagc gggaacatgt ggctgggcgg
    20341 cggcacgctt tatctctatg cgttgggcat gggcctgccg ctgatgctaa ttaccgtctt
    20401 tggtaaccgc ttgctgccga aaagcggccc gtggatggaa caagtcaaaa ccgcgtttgg
    20461 ttttgtgatc ctcgcactgc cggtcttcct gctggagcga gtgattggtg atgtatgggg
    20521 attacgcttg tggtcggcgc tgggtgtcgc attctttggc tgggccttta tcaccagcct
    20581 acaggctaaa cgcggttgga tgcgcgtggt gcaaataatc ctgctggcag cggcattggt
    20641 tagcgtgcgc ccacttcagg attgggcatt tggtgcgacg cataccgcgc aaactcagac
    20701 gcatctcaac tttacacaaa tcaaaacggt agatgagtta aatcatgcgc tcgttgaagc
    20761 caaaggcaaa ccggtgatgt tagatcttta tgccgactgg tgcgtcgcct gtaaagagtt
    20821 tgagaaatac accttcagcg acccgcaggt gcaaaaagcg ttagcagaca cggtcttact
    20881 tcaggccaac atcacggcca acgacgcaca agatgtggcg ctgttaaagc atcttaatgt
    20941 ccttggccta ccgacaattc tcttttttga cggacaaggc caggagcatc cacaagcacg
    21001 cgtcacgggc tttatggatg ctgaaacctt cagcgcacat ttgcgcgatc gccaaccgtg
    21061 aacgacactt gcagcgggaa agacggagga aaatagcgtg caacgtgaag atgtactggg
    21121 agaagccctg aaattattag aattacaagg gattgccaac accacgctgg agatggttgc
    21181 tgaacgtgtg gattatccac tggacgagct acgccgcttc tggccagaca aagaggcaat
    21241 cctctacgat gcgctgcgtt atcttagcca acagatagat gtctggcgtc gtcagctgat
    21301 gctggacgaa acgcaaaccg ccgaacaaaa gcagctggca cgttatcagg cgttatcgga
    21361 gtgcgttaaa aacaaccgct atccgggctg tctatttatc gctgcctgta cgttttatcc
    21421 cgatcctggc caccctattc atcaactggc cgatcagcaa aaaagcgcgg cctacgattt
    21481 cacccacgaa ctgttaacca cactggaagt tgacgatccg gcgatggtag caaagcagat
    21541 ggaactggtg ctggaaggct gtttaagccg aatgctggtg aatcgtagcc aggcggatgt
    21601 cgacactgca catcggctgg cggaagatat cctgcgcttc gcccgctgcc gccagggtgg
    21661 tgcattgacc tgacagaaac acagaaaaga agcgatttgc cgcaatctta agcagttgaa
    21721 tcgcttttac tgaaattagg ttgacgagat gtgcagatta cggtttaatg cgccccgttg
    21781 cccggatagc tcagtcggta gagcagggga ttgaaaatcc ccgtgtcctt ggttcgattc
    21841 cgagtccggg caccaaattc atatcaacgg acctctacgg aggttcgttt ttcgtttcag
    21901 aacgccacga tttaagcgtt ctgcctacaa atcaattcta ccgaactcaa ccagattccc
    21961 cccacatcaa ccccattgtg tgggtataat tgcgggcata ccccagttcg acagaatttg
    22021 taccctcttt aacgcattca gaaggctgaa ctatggcact gactgacgca aaaatccggg
    22081 ctgcaaagcc cactgacaag gcttataaac tcactgacgg gactggcatg ttcctgctgg
    22141 tacatcctaa tggctcccgt tactggcgtc tccgttatcg tattctgggt aaggagaaga
    22201 ctctggcact tggtgtgtat ccagaagttt ctctctccga agctcgtaca aaacgggatg
    22261 aggcccgaaa actgatttcg gagggggttg acccttgcga acagaaaaga gctaaaaaag
    22321 tagtccctga tttacagctc tcttttgaac atattgcgcg acgctggcat gccagtaata
    22381 aacaatgggc acaatcacac agcgataaag tactcaaaag cctcgagact cacgttttcc
    22441 cctttatcgg caaccgggat atcacaacac tcaatacccc ggatctgctt atccctgttc
    22501 gtgctgcaga agctaaacaa atttatgaaa tcgccagtcg tctgcagcaa agaatatctg
    22561 ccgtaatgcg ttatgccgta cagtctggca tcatcagata taatcctgct ctggatatgg
    22621 ctggcgcatt gactacggta aaacgccagc atcgccccgc tcttgatctt tctcgcctgc
    22681 ctgaactttt gtcgcgtatt ggcagttaca aagggcaaac tgtcacccag cttgccgtta
    22741 tgctgaattt actggttttt attcgttcca gtgaactcag atacgcccgc tggtctgaaa
    22801 ttgatattga caatgccatg tggactattc cagccgaacg cgaacccctg ccaggcgtaa
    22861 aattctcaca ccggggctcc aagatgcgaa caccacatct tgtgccactc agcaaacagg
    22921 ctgtagccat actgacagaa ctacagacat gggctggtga aaatggtctg atctttacgg
    22981 gagcacatga cccgcgtaaa ccaatcagtg aaaatgtaat gaacgcatc
//
LOCUS       DAJVUJ010000053        22955 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.053, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000053 DAJVUJ010000000
VERSION     DAJVUJ010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22955
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.053"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(36..779)
                     /gene="fsr"
                     /locus_tag="OQQ48_001899"
                     /pseudo
     CDS             complement(36..779)
                     /gene="fsr"
                     /locus_tag="OQQ48_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
     gene            997..2649
                     /gene="ushA"
                     /locus_tag="OQQ48_001900"
     CDS             997..2649
                     /gene="ushA"
                     /locus_tag="OQQ48_001900"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCS1952694.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDVEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQAPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVTKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(2686..3165)
                     /gene="ybaK"
                     /locus_tag="OQQ48_001901"
     CDS             complement(2686..3165)
                     /gene="ybaK"
                     /locus_tag="OQQ48_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HCS1952695.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMAAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(3369..4163)
                     /locus_tag="OQQ48_001902"
     CDS             complement(3369..4163)
                     /locus_tag="OQQ48_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HCS1952696.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMTPLPTRLLKKLKNADALIVEADVSTSDTPFANLPTCEALEERINEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQITMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            4301..4642
                     /locus_tag="OQQ48_001903"
     CDS             4301..4642
                     /locus_tag="OQQ48_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCS1952697.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSTL
                     INNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(4857..7361)
                     /gene="copA"
                     /locus_tag="OQQ48_001904"
     CDS             complement(4857..7361)
                     /gene="copA"
                     /locus_tag="OQQ48_001904"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HCS1952698.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARVVTAEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGESVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALI
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNPALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKVVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            7623..8555
                     /gene="glsA"
                     /locus_tag="OQQ48_001905"
     CDS             7623..8555
                     /gene="glsA"
                     /locus_tag="OQQ48_001905"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HCS1952699.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAVVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENIEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGLN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            8558..9850
                     /locus_tag="OQQ48_001906"
     CDS             8558..9850
                     /locus_tag="OQQ48_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCS1952700.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVAHAFGAYAVQFLHEGSQEEHLILLYALGSIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGSLLLSLIVAMVMKRNKTV"
     gene            9975..10382
                     /gene="cueR"
                     /locus_tag="OQQ48_001907"
     CDS             9975..10382
                     /gene="cueR"
                     /locus_tag="OQQ48_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HCS1952701.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(10383..10841)
                     /locus_tag="OQQ48_001908"
     CDS             complement(10383..10841)
                     /locus_tag="OQQ48_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HCS1952702.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(10838..11755)
                     /locus_tag="OQQ48_001909"
     CDS             complement(10838..11755)
                     /locus_tag="OQQ48_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCS1952703.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            11901..12578
                     /gene="fetA"
                     /locus_tag="OQQ48_001910"
     CDS             11901..12578
                     /gene="fetA"
                     /locus_tag="OQQ48_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HCS1952704.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNRQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            12565..13344
                     /gene="fetB"
                     /locus_tag="OQQ48_001911"
     CDS             12565..13344
                     /gene="fetB"
                     /locus_tag="OQQ48_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HCS1952705.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDIFWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(13407..14261)
                     /gene="cnoX"
                     /locus_tag="OQQ48_001912"
     CDS             complement(13407..14261)
                     /gene="cnoX"
                     /locus_tag="OQQ48_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HCS1952706.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQMAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(14322..15131)
                     /gene="ybbO"
                     /locus_tag="OQQ48_001913"
     CDS             complement(14322..15131)
                     /gene="ybbO"
                     /locus_tag="OQQ48_001913"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCS1952707.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNNMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(15121..15744)
                     /gene="tesA"
                     /locus_tag="OQQ48_001914"
     CDS             complement(15121..15744)
                     /gene="tesA"
                     /locus_tag="OQQ48_001914"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HCS1952708.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            15715..16401
                     /locus_tag="OQQ48_001915"
     CDS             15715..16401
                     /locus_tag="OQQ48_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS1952709.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            16398..18809
                     /locus_tag="OQQ48_001916"
                     /pseudo
     CDS             16398..18809
                     /locus_tag="OQQ48_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            19238..22955
                     /locus_tag="OQQ48_001917"
                     /pseudo
     CDS             19238..22955
                     /locus_tag="OQQ48_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
BASE COUNT         5294 a         6008 c         6489 g         5164 t
ORIGIN      
        1 ataaaacact atcagtaagt tggagtcatt acctagctgc tgatgctcgc catatagaaa
       61 tatttcgaga aaatgaggat taacagaatg ctgactgcca gtaccacttt gttgcgcggc
      121 agtggattga ttatcgtcgc tttgggtttt cctttattca ttcggtgctg tgccgagtac
      181 caacggctaa tttgcgccaa caccacgatc gccagcagtg ccgcaagcac aaaccaggca
      241 acgttgcctt tgccataagg cgcgataatc accgccgtca gcaagggtcc cagggaactg
      301 ccaaagttgc cgccgacctg aaagatagat tgcgccaggc catgccgccc gccggaagcc
      361 atacgggcta cgcgagaaga ttccggatga aagaccgatg aactggtacc gaccagcgcc
      421 gccgccagca gaactgcgcc aacactgccc gccagcgcaa gcagcaccag accacttaag
      481 gtaaagcaca tgccaattgg caacgaccac ggcatcggat atttatcggt ccagtagccg
      541 accactggtt gcagtagcga agaggcgagc tggaaggtga gggttatcat gccaatctgc
      601 ataaatgtca gagaaaattc tgactgaagc agaggataaa tcgccagaat cagcgattgg
      661 atcatgtcgt tcagcagatg tgagaggctg atagcaccta aaataccaaa cgatgttcgg
      721 gccttggtcg ttgacgcagc cgcgcccgcc acaggctggg tttgttcact cattgccata
      781 ggaaagtcac tttttcaggg ttgcgatgta aagaatgatc ttatttgtga ttattacccg
      841 actaacatac ctgtaagcgt cgcctgaagg aagtctcaac gccgaataca gaatttctaa
      901 tctggatgca gatttatctt caccggacgc agacttgtct attatgttgc gtcatactat
      961 ttttcaacac gttgaaatca ggtcagggag agaagtatga aattattgca gcggggcgtg
     1021 gcgttagcgc tgttaaccac atttacactg gcgagtgaaa ctgctctggc gtatgagcag
     1081 gataaaacct acaaaattac agttctgcat accaatgatc atcatgggca tttttggcgc
     1141 aatgaatatg gcgaatatgg tctggcggcg caaaaaacgc tggtggatgg tatccgcaaa
     1201 gaggttgcgg ctgaaggcgg tagcgtgctg ctactttccg gtggcgacat taacactggc
     1261 gtgcccgagt ctgacttaca ggatgccgaa cctgattttc gcggtatgaa tctggtgggc
     1321 tatgacgcga tggcgatcgg taatcatgaa tttgataatc cgctcaccgt attacgccag
     1381 caggaaaagt gggccaagtt cccgttgctt tccgcgaata tctaccagaa aagtaccggc
     1441 gagcgcctgt ttaaaccatg ggcgctgttt aagcgtcagg atctgaaaat tgccgtcatt
     1501 gggctgacaa ccgatgacac agcaaaaatt ggtaacccgg aatacttcac tgatgtcgaa
     1561 tttcgtaagc ccgccgatga agcgaagctg gtgattcagg agctgcaaca gacagaaaag
     1621 ccagacatta ttatcgcggc gacccatatg gggcattacg ataatggtga gcacggctct
     1681 aacgcaccgg gcgatgtgga gatggcacgc gcgctgcctg ccggatcgct ggcgatgatc
     1741 gtcggtggtc actcgcaagc tccggtctgc atggcggcag aaaacaaaaa acaggtcgat
     1801 tacgtgccgg gtacgccatg caaaccagat caacaaaacg gcatctggat tgtgcaggcg
     1861 catgagtggg gcaaatacgt gggacgggct gattttgagt ttcgtaatgg cgaaatgaaa
     1921 atggttaact accagctgat tccggtgaac ctgaagaaga aagtgacctg ggaagacggg
     1981 aaaagcgagc gcgtgcttta cactcctgaa atcgctgaaa accagcaaat gatctcgctg
     2041 ttatcgccgt tccagaacaa aggcaaagcg cagttggaag tgaaaatagg cgaaaccaat
     2101 ggtcgtctgg aaggcgatcg tgacaaagtg cgttttgtac agaccaatat ggggcggttg
     2161 attctggcag cccaaatgga tcgcactggt gccgactttg cggtgatgag tggaggagga
     2221 attcgtgatt ctatcgaagc aggcgatatc agctataaaa acgtactgaa agtgcagcca
     2281 ttcggcaatg tggtggtgta tgccgacatg accggtaaag aggtgattga ttacctgacc
     2341 gccgtcgcac aaatgaagcc agattcaggt gcctacccgc aatttgccaa cgttagcttt
     2401 gtgacgaaag acggcaaact caacgacctg aaaatcaaag gcgaaccggt cgatccggcg
     2461 aaaacttacc gtatggcgac attaaacttc aatgccaccg gcggtgatgg ctatccgcgc
     2521 cttgataaca aaccgggcta tgtgaatacc ggctttattg atgccgaagt gctgaaagcg
     2581 tatatccaga aaagctcgcc gctggatgtg agtgtttatg aaccgaaagg tgaggtgagc
     2641 tggcagtaat ccgaaagtgc cggatatttg catccggcac aatgcttaat cgcggcgggc
     2701 gatatcagca aatttggcat cgaggatctt cgccagatcg cctgccgcca gttcgatatc
     2761 cagtccgcgc ttgccgccag aaacataaat agtggcaaat tcttgtgcgg gggcgtcgat
     2821 aatcgtcggc agacgttttt tctgccccag tgggctaatc cccccaacca ggtatcccgt
     2881 cgaacgctgc gcggccatcg gatcggccat ctcaactttc ttggcaccca gcgcttttgc
     2941 tactttttta agatccagtt gacctgcgac cggcgtaacg gccactgcca gatgtttcat
     3001 atcaccgttc actgccacca gtagcgtttt gtagacttga tccggattca aacctaattt
     3061 tttgacgact tcatcgccaa aattggtttc agccggatcg tgctcgtagg tatggatttg
     3121 aaacgaaatc ttgttttttt cgagtaattt aactgcgggt gtcatggtaa ttttcctgcc
     3181 ttaaacaaaa aaacgaagca agcatacgcc tgatggcggt ccaaaaaaaa gagtcatctt
     3241 gcctaagagt attggcagga tggtgagatt gagcgacaat cgagttacac cgtcgcttaa
     3301 agtgacggca taataataaa aaaatgaaat tcctctttga cgggccaata gcgatattgg
     3361 ccattttttt agcgcaacat ttgcggcaaa ttcccttctc catacaggtg tagtgcaccg
     3421 accgcgacca catatcgccc cggcggcatg gcgcgtaatt tatcccgcca ggcgagattt
     3481 cgctgatgca tcagcacatc gtacagcgac tgactgaacg tattgggcag cgttatatca
     3541 ttattttgcg gaggtgcatt cagccaccag ctcatcattt gttgcagcaa ccgtgcgttg
     3601 gtatgccagt gggtcagcgt atcgtccagc agcgccagtc ctttgtcagg gagctggagc
     3661 aacatggtaa tctggttttc agccccttcc agttcaatca cgggtttatg ttgttgcttc
     3721 gccgcctgca ataactggta atcgataccg tattctgccc gcagccccag tttttgtgcc
     3781 tgcgtcgcct gaagaaccat cgcgatttgc cacagcggtt gagtagaaaa gagcgacgga
     3841 gaaatgccca tctcctggct aatgtgctgc agattttgga gttgctcctc gttaatgcgc
     3901 tcttccagcg cctcgcaagt aggcaaatta gcaaaaggcg tatcgctggt ggaaacatcc
     3961 gcctcgacga tcagcgcatc ggcgtttttg agctttttga gcaaacgggt gggcagggga
     4021 gtcatatcgt ggctacccat atgaatactg ccaatcagat gaaaatggcg attgccggga
     4081 agggtgatat cgatggcagg ccaggtgtaa tgattaccgc gcagcgcggc ccaaagcgtt
     4141 tttacccggt acaacagatc catacgacct ccctttgtga aatatcatgc tagcgcgcgg
     4201 tgagggatgg cgcaaatgga gttggggact tgtttaatgt gtttgtatga ttcagtatgt
     4261 tcttgcatcg ctattcacag ggaagcaaca gttaaaaacc atgaaacagg caacaagaaa
     4321 accgacgaca cccggagata ttcttctcta tgaatatctg gaaccgctcg atttgaaaat
     4381 caatgagtta gcagagttgc tgcatgttca tcgtaatagc gtcagtacac tgatcaataa
     4441 taatcgtaaa ctcaccactg agatggcatt ccgtctggcg aaagtttttg ataccacagt
     4501 cgatttttgg ctaaacctcc aggcggcagt tgatctttgg gaagttgaaa acaacatgcg
     4561 cacccaggaa gaattgggac ggattgaaac agtggctgaa tatttggcac gccgtgaaga
     4621 gcgtgcaaaa aaggtcgcgt aaggccaaaa catcgcttca tctcgtaggc cggataaggc
     4681 gttcacgccg catccggcat ccgagcacca ttgcctgatg cgacgcttac ccgtcttatc
     4741 aggcctacaa acctgtgcct gaaccgtagg ccggataagg cgtttacgcc gcatccggca
     4801 gtcatgcgtc gatgccaaat gcgccgccct aaagcagcgc atccgcaatg acgtacttat
     4861 tccttcggtt taaaccgcag caaccggttg gcgttactca ctacggtaat cgacgagagc
     4921 gccattgccg ctccggcaac taccgggtta agcagtgttc cagtaaacgg ccacaaaata
     4981 ccagcggcga ccggaatacc aatgctgttg tagataaacg caccgagcag gttctgcttc
     5041 atattgcgca gcgttgcgcg ggaaatcgcg agcgcatctg caacacccat caggctatgg
     5101 cgcatcaggg taatcgccgc ggtttcaatg gcaacatcac tgccgccacc catcgcaata
     5161 ccgacatccg cctgagccag cgctggcgcg tcgttaatgc cgtcgcccac cattgccacc
     5221 tgacgtcctt cactttgcag acgtttgatc gcttcggctt taccgtctgg cagcaccccg
     5281 gcgattacct catcaatccc tgcttctttg gcgatcgcat tggcggtggt tgggttatcc
     5341 ccggtcaaca tcaccagacg atatcccgct ttatgcaggc gttgcagcgc cgccacgcta
     5401 tcactacgca acggatcgcg tacagccagc agggctacta ctttcccgtc aatcgccagc
     5461 agcacaggcg ttgctccttg cgatgcctga gctgaaatat cggcttcaat cgctttagta
     5521 tcgacttgct ggtcatttaa cagcgccgga ttacccagca ataacgcatg accttcagct
     5581 tcaccgctta cgcccagccc gcgcaatgtg cggaaaccgt tgacctgcgg cagctgcata
     5641 tcgcctgctt tatcgaggat cgctcgtgcc agcggatggc tggaaccttg ctccagcgcc
     5701 gccgccagac gcaatgcttg cgcttcatca acatcagcaa atgtttttac tgcgacaacc
     5761 tgcggcttcc cttcggtcag cgtcccggtt ttatcgaaca cgacggtatc gagtgtactg
     5821 gcgcgttgca gcgcgtcggc gtcccgcacc agcacgccaa actcagccgc ccgcccgacg
     5881 ccggaaataa tcgacattgg cgtcgccagc cccagcgcac acggacaggc aataatcagc
     5941 accgtggtgg ctatcaccag ggtatagaca atctgcggtg ccggaccaaa gaaataccag
     6001 attgctgcac tgatcagcgc aatcaccact actaccggca caaatacggc tgagatttta
     6061 tccgccagct gaccgatttc tggcttgctg ctctgggcct ggcgcaccat gcgaataatc
     6121 cttgacagcg tggtatggct gccaaccgca ctggcgcgaa atagcacgct accgtcctgt
     6181 accactgtcc cggcatggac gctttcgcct tcgccttttt gctgcgggat tggttcgccc
     6241 gtcagcatcg cttcatccag ccatgcttcg ccctgggtaa tctcgccatc taccggcacg
     6301 cgatcgccgg tcgtcaggcg cagtaacatc cctggctgaa cttccgccag cggcacgctt
     6361 ttttcaccct ctgcggtgac cactcgcgcc gtcggtgggg ttaaatcgag taatttctcc
     6421 agcgccttag aagaacgctg acgcgcgcgc gcttccagca tatggccgag attaatcaga
     6481 ccgataatca tcgcactggc ttcatagtaa agatgccgcg cttccatcgg gaaccactgc
     6541 ggccacaggt tgacgctcat cgaatagagc cacgccacac ccgtacccag cgccaccagc
     6601 gtatccatcg tcgccgcacc gttcagcagg cttttccacg cgctgcggta aaaatgcccg
     6661 ccggcgaaaa ccattaccgc cagggttatc aggccgataa ctaaccacag gctgcggttg
     6721 tcagcggtga ccatcatgtt atcgccgatc atcccccaga ccattaccgg aatacccacc
     6781 gccagtgcga caatcgcctg ccagcggaag cgcttcatcg tagcgacggc ggtttcctgc
     6841 tggcgctcgc ggcgtttcgc gtcatcttca atcgcttccg cgccgtagcc cgctttttcc
     6901 accgcctgca ctaaatcttg tggggaggca ctgcccatca ccagcgcagt acgctccgcc
     6961 aggtttaccc gtgcctgagt gacgcccggt acgctttgca gcgcattttg tacgcgggtg
     7021 acacagctgg cgcagctcat gccgctcagc agcaactgct ggctgtcatc gtcatcggcg
     7081 gtcgctgccg gaagcgcctc agaaaccgct gtcagtgctt ccgacgggat tgatgactcc
     7141 gccagcggtt tagcctttgg gtggcttaca gatgcgtcat aacccgcttg tttgatggtt
     7201 tcgatcagct gttctgcact ggcagtcccg gtaacgtgcg cttcagtgat agacacatcc
     7261 gcttgttcca catccgggcg ctgttcgaga ctttctttca cgcgtttaac gcagtgaccg
     7321 caggacaggc cgtccagggt caggtcgata gtttgtgaca taaaacactc ctttaagaca
     7381 gttttgactg gctgtgataa aggttaaacc ttccagcaag gggaaggtca agaaattaat
     7441 aaaccaggcg ggtaaaagtc cgtaaagatt aaaaaatcgg ctcgatttgc atcaggatta
     7501 gacatttatt tctttgtttt cctgtagtta agttgctggt gctaagttaa atcaggatgc
     7561 ctgaaaatcg gcaccggggt gaggaattac ctcccgcatc tataaaaagg agttaacaaa
     7621 agatgttaga tgcaaacaaa ttacagcagg cagtggatca ggcttacacc caatttcact
     7681 cacttaacgg cggacaaaat gccgattaca ttccctttct ggcgaatgta ccaggtcaac
     7741 tggcggtagt ggctatcgtg acctgcgatg gcaacgtcta tagtgcgggt gacagtgatt
     7801 accgctttgc actggaatcc atctcgaaag tctgtacgtt agcccttgcg ttagaagatg
     7861 tcggcccgca ggcggtacag gacaaaattg gcgctgaccc gaccggattg ccctttaact
     7921 cagttatcgc cttagagttg catggcggca aaccgctttc gccactggta aatgctggcg
     7981 ctattgccac caccagcctg attaacgctg aaaatattga acaacgctgg cagcgaattt
     8041 tacatatcca acagcaactg gctggtgagc aggtagcgct ctctgacgaa gtcaaccagt
     8101 cggaacaaac aaccaacttc cataaccggg ccatagcctg gctgctgtac tccgccggat
     8161 atctctattg tgatgcaatg gaagcctgtg acgtgtacac ccgtcagtgc tccacgctca
     8221 tcaatactgt tgaactggca acgcttggcg cgacgctggc ggcgggtggt ctgaatccgc
     8281 tgacgcataa acgcgttctt caggccgaca acgtgccgta cattctggcc gaaatgatga
     8341 tggaagggct gtatggtcgc tccggtgact gggcgtatcg cgttggttta ccgggcaaaa
     8401 gcggtgtagg tggcggtatt ctggcggtcg tccctggcgt gatgggaatt gccgcgttct
     8461 caccaccgct ggacgaagaa ggtaacagtg ttcgcggtca aaaaatggtg gcatcggtcg
     8521 ctaagcaact cggctataac gtgtttaagg gctgatcatg atgaacacgg aaggtaataa
     8581 cggtaacaaa cccctcggtc tatggaatgt cgtttccatc ggtattgggg caatggtggg
     8641 ggcggggatc ttcgcgctgc tggggcaggc tgcgttgcta atggaagcct cgacctgggt
     8701 cgcctttgct tttggcggta ttgtggcgat gttttccggt tatgcctatg cgcgtctggg
     8761 ggcgagctat cccagcaatg gcggcattat cgacttcttt cgtcgcggat taggcaacgg
     8821 cgtcttttcg ctggcgctct cgttactgta cctgttgacg ctggcggtga gcatcgccat
     8881 ggtcgcccat gcttttggcg cttatgccgt gcagtttttg catgaaggca gccaggagga
     8941 gcaccttatt ttgctctacg cgttggggag cattgcggtg atgacgcttt tcaactcctt
     9001 aagcaaccat gcggtagggc ggctggaagt gatcctcgtc ggcattaaaa tgatgatcct
     9061 gttattgctg attattgccg gtgtctggtc gctgcaacca gcgcatattt ccgtctctgc
     9121 gccccccagc tccggtgcgt tcttctcctg tattgggata actttccttg cctatgcggg
     9181 ctttggcatg atggcgaacg cggcggataa agtgaaagat ccgcaggtca ttatgccacg
     9241 ggcgtttctg gtggcgattg gcgttaccac gttgctttat atctcgctgg cactggtttt
     9301 gcttagcgat gtatcggcat tagagttaga aaaatatgcc gataccgccg tagcgcaggc
     9361 tgcttctccg ctgctcggac atgtgggtta tgtgatcgtc gtcatcggcg ctttactggc
     9421 gacggcttca gccattaacg cgaacctgtt cgccgtgttt aacatcatgg acaacatggg
     9481 cagcgaacgc gaactgccga agctaatgaa taaatccctg tggcggcaga gtacctgggg
     9541 caacatcatt gtcgtggtgt tgattatgct gatgacggcg gcactgaatt taggctcact
     9601 cgccagcgtt gccagcgcca cctttttgat ttgctacctg gcggtgtttg tggtggcgat
     9661 ccgcctgcgt catgatattc acgcctcgtt gccgattctt atcgttggta cgttggtgat
     9721 gttgttggtg atcgttggct ttatctacag tctgtggtcc cagggtagcc gtgcgttgat
     9781 atggattatt ggctcactct tactcagcct tattgtggca atggtcatga agcgcaataa
     9841 aaccgtataa catctctctg tgcgcagtac ttcctgtatt attgtggtgg cggtcgatat
     9901 tcgcactggc aaaaaaacgt gcttgaatat ctgttgaaac cctttaacaa agcacaggag
     9961 gcgttgcgcg aacgatgaac atcagcgatg tagcaaaaat taccggcctg actagcaaag
    10021 ccattcgctt ctatgaagag aaggggctgg tgacgccgcc gatgcgcagc gaaaacggtt
    10081 atcgcaccta cacgcagcag catctcaacg aactgacctt actgcgccag gcacggcagg
    10141 tgggctttaa cctggaagag agcggcgagc tggtgaatct gtttaacgac ccgcagcggc
    10201 acagcgccga cgtcaaacgg cgcacgctgg agaaggtggc ggagatcgaa cgacacattg
    10261 aggagctgca atccatgcgc gaccagctgc tggcactggc gaatgcctgc cctggcgatg
    10321 acagcgccga ctgcccgatt atcgaaaatc tctccggctg ctgtcatcat cgggcagggt
    10381 gattaagacg agacggctcg gatatgtagg gttatccctt ctatcgcaat gacttcaaca
    10441 tgcgtacctg caccgagatc ctcgctggcg ctgacaggcc atgaactgtc accgacgcgc
    10501 atatgaccgc gcccgttgac cagcggagat tccagcacaa aacgtcggcc aatcagctgc
    10561 tgcccgcgct ggtttaaatg actatcgctg tgcttttgtt cgcgcacccg ccgcgacaac
    10621 catttccacc acagccaggc ggcgagcagc gtcaggatgg caaacattac cccttgccac
    10681 tcccaaccca gcggcaccag ccagaccacc aggccggtaa tcaccgctgc cacgccgctc
    10741 cacaacaaat aaccatttcc gcccagcatc tcggctgcca gcagcaaacc gccgagactg
    10801 agccagaaaa tatgtggatg aacgaccatt aactccatca tggctgagtc cgtttgttgg
    10861 cgctgtcttt caccagttcg gcaatcccgg caatcgaccc catcaggctg ctggcctcta
    10921 atggcatcat cactactttg ctgttactgg aggaaccgat ctgctgtaac gcttcggtgt
    10981 atttctgcgc tacgaagtag ttcaccgcct gaatatcacc ggaggcgata gcttcagaca
    11041 ccattttggt ggcgcgggct tctgcttcgg cggaacgttc acgcgcttca gcctgtaaaa
    11101 acgccgactg acgttcgcct tccgctttca ggatttgcga ctgtttttca ccttcggctt
    11161 tgaggatttc cgcctgacgg atcccttccg cttcaagaat gtaagcgcgt ttggtacgtt
    11221 ccgctttcat ctgcgcgttc attgaagaga taagctctgc cggtgggcgc acgtcgcgaa
    11281 tttcaatacg ggtgacttta atcccccacg ggttggtggc ttcatcgaca atacgcagca
    11341 ggcgtgagtt gatgctgtcg cgctgagaga gcatttcgtc aagttccatt gaacccaaca
    11401 cggtacggat gttagtcatg gtcaggttga tgatcgccag ctccagattg ctgacttcat
    11461 aggcagcgcg tggcgcgtca atcacctgaa taaaacagac tgcgtcgatg gtaacgttgg
    11521 cgttatcttt cgagataact tcctgggaag ggatatcgag cacttgctcc atcatattga
    11581 tcttgcgacc aatgcgatcc ataaacggca ccaccagact gagccccggc tgtaacgttt
    11641 tggtatagcg accaaaacgt tctaccgtcc attgatagcc ctgcggcacg attttgacgc
    11701 ccgcgccgac aatgaccagc gcgacaaaaa tgagaatcgg gataaagata agcatcggaa
    11761 aaacctcctg ttgtaccgtc cataatcagc aaaattgctg cttgattaaa caaattatac
    11821 ctgattactg aaagagagtt cccccttatt cctgcgaagg ataaactgtt tttagtaaaa
    11881 atcagaaaaa gggaacagcg atgcaggaaa atagtccttt gcttcagcta caaaacgtag
    11941 gatatctggc gggtgatgcg aagattctta ataacatcaa tttttcgctg cgtgctggcg
    12001 aatttaagtt aattaccggt ccttctggtt gtggcaaaag tacgctgcta aaaatagttg
    12061 cttcattgat cagcccaacc agcggaacgt tactgtttga aggtgaggat gtcagcacac
    12121 taaagccaga aatctaccgc caacaagtct cttactgcgc ccagacaccg acgctgtttg
    12181 gcgatacggt atacgataat ctgatctttc cctggcagat ccgtaaccgg cagcctgacc
    12241 cagccatttt tctcgatttt ctcgaacgct tcgccttgcc ggacagcatt ttgacgaaaa
    12301 atatcgccga gctatctggt ggtgaaaaac aacgcatctc actgattcgt aacctgcaat
    12361 ttatgccgaa ggttttattg ctggatgaaa tcaccagtgc gctggatgaa agtaataaac
    12421 ataacgttaa tgagatgatc catcgttatg tgcgcgagca aaatattgcc gtgctgtggg
    12481 tgacacacga taaagacgaa attaatcatg cggataaagt gattacactg caaccgcatg
    12541 ccggagaaat gcaggaagca cgctatgaac tcgcataata ttactaacga atcattggca
    12601 ctggcattaa tgctggtggt ggtggcaatc ttaattagcc ataaagaaaa actggcgctg
    12661 gagaaagata ttttctggag cgtcgggcgc gcgataattc agctgattat tgtcggctat
    12721 gtgctgaagt atattttcag cgtggatgat gccagcctga cattattgat ggtgttattt
    12781 atctgcttta atgcggcgtg gaacgcgcaa aaacgcagta aatatattgc taaagctttt
    12841 atctcatcgt ttattgccat tacggttggg gcgggaatta ccctggcggt gctgattctc
    12901 tcagggtcga ttgaatttat cccgatgcag gtgatcccta tcgccgggat gattgccggt
    12961 aacgccatgg tagcggtggg gttgtgttac aacaatttag ggcaacgggt cattagcgaa
    13021 cagcaacaga ttcaggagaa actgagtctt ggcgcgacgc cgaagcaggc ttcagcgata
    13081 ttgattcgcg acagtattcg cgcggcttta attccgacgg tcgattcagc aaaaacggtt
    13141 ggcttagtga gtttaccagg aatgatgtcc gggctgatat ttgccgggat tgatccggtg
    13201 aaggcgatta aatatcagat tatggtgacc tttatgctgc tctcaaccgc cagcttgtcg
    13261 accattattg cctgctattt aacctatcgt aagttttata attcgcgcca ccagttggtg
    13321 gtgacgcaat tgaagaagaa atgatgcgat gccggatgcg cagcatcgca tccggcgttg
    13381 tggttcatgt gccgaacaac ggaatatcaa tacaacaatg catacagctg gcggcgatac
    13441 ttcgacgcca gtgcatcacc cgtacccagc gcagcgagga tctcctggaa cgttttacgc
    13501 gtctgaccgt ctgcggcggt gagatcttta cgcagatgcc cgaacagcaa ctccagcgcc
    13561 tcttcattgc gcccaacctg atgcagttgc agcgccagtt gcgtcgccag tgcggcatct
    13621 tctggattct ccgccatctg ctgttgcaac tgttgaattt ccggcgtatc agccgcctgc
    13681 ttcagcagtt cgatttgcgc caccagcccc tggtagcggg tgtcctgatc ctgcaacgga
    13741 atggttttca gcaccgcttc cgcatcttca gaacggttca gcgcaatcag cgtttctgcc
    13801 agcagcaggc cgatctcccc gttctgattc gacaactgcc aggcgtcttt cagcaatggc
    13861 agggcatcgg tgtaattgcc ttcctgcatc agttgcatcg cctgctgcgc tttcagctct
    13921 tcttcgcgcg gcagcacttt atccagcagg gcgcggatcg cctcttccgg ttgcggcccc
    13981 tggaagccat ctaccggttg cccgttctgg aacagataca cggtcggaat cgcgcgcaga
    14041 ccaaactggg cggcaatcat ctgctccgcg tcgcagtcca gcttcgccag aataaattgc
    14101 ccgttgtact gcgccgcgag gctttccagg atcggggtta actgcaaaca gtgctggcta
    14161 cgttcagacc aaaaatagaa cagcaccgga gtggtcatcg actgttcaag aacctgttgc
    14221 aggttagatt cgttaatgtt gacaatattt tctacggaca tggagtcgct ctctgttgtc
    14281 gatattttct ttgacatggg ggcttaagcg cgcgcttcaa ctcacccctg caatattttg
    14341 tccatcacgc gcccaggcag caggcgcttg agcaccatta ctgcccaggt caccagcgtt
    14401 accggatagc gcatcttcgg cttctcgcta ataaaagcat ggcgcacttt gtcgaccacc
    14461 gcttccggtc ccaacgtaaa gcgggcggcg atgccgggat tttcgactgg tttatcactt
    14521 tgcgtctggt tgacgttgtc ggtgaagcga gtacgaatgg gaccgggttc gatcaggctg
    14581 actttaattc cgctgtggcg tagctccatg cgcagcgcgt ctgaccacgc ctccagcgca
    14641 tatttactgg ccgcgtaagc gccacgaccc ggcgtggaga ttaatcccat caccgatgat
    14701 gtcatcacaa tacgcccttc accgtgcggt aacatcgcgg gtaacaggcg catggtgagc
    14761 tggtgtgcgc cgaaaaagtt ggcggaaaac tgctgttcca tctgcgcacg gctgatggtg
    14821 gaaagggggc catacatgcc gaatccggca ttgttaaaga tcccatacag acaattatcg
    14881 gtcagggcga tcacctcgtc tgctgcgcga tcaacacttt ctggtgaatc cagatcgatc
    14941 aacacgccgg taaatcccat gttgttcata cgctcaacat catccggttt tcggcaacct
    15001 gccagcacat gaaaaccctg gcgttttaat tcgagcgcgc tttccaggcc aattccactg
    15061 gaacatccgg taattaagac cgatttttgc ataactttac ctgtcaggat ctccgttgct
    15121 ttatgagtca tgatttacta aaggctgcaa ctgcttcgcc atccagtcgg caataaacgg
    15181 ctgggcgtcg cggttgggat gaataccgtc atcctgcatc cattgtggct tgaggtagac
    15241 ctcttccata aaaaagggca gcagcggaac atcaaactct ttggcgagtt tggggtaaat
    15301 ggcgctaaag gcttcattat aacggcgacc atagtttgca ggcagacgta tttgcattaa
    15361 caatggttca gcgttggcgg ctttgacatc ctgcaaaatc tggcgcagcg tttgctcggt
    15421 ttgctgtggc tgaaaaccac gcaaaccgtc attgccgccc agttcaacca gcacccagcg
    15481 cggctgatgc tgtttcagca gagccggaag gcgcgccagt ccttgttgcg aggtgtcgcc
    15541 gctgatgctg gcattaacta ccgacgtttt actctgccac ttatcattca acaaggcagg
    15601 ccaggccgcg ctggcagaca ttcgataccc ggcgctcagg ctatcaccca gaatcaataa
    15661 cgtgtccgct gcggcggcac ggaaggttaa caggaccagg aacaggaagg gcaaatgcca
    15721 gcggaaaaca ttgttgaagt tcatcatctt aagaagtccg tcggtcaggg ggagcatgaa
    15781 ctctccatcc tcaccggagt tgagctggtt gtcaaacgtg gcgagaccat cgcactggtg
    15841 ggcgagtcgg gatcgggtaa gtcaaccttg ctggcgatcc tcgccgggct tgatgacggc
    15901 agcagtggcg aagtgagtct ggtgggacaa ccgctacata atatggacga agaagcgcgg
    15961 gcaaagttgc gcgcgaagca cgtcggcttt gtttttcagt catttatgtt aattcctacc
    16021 cttaacgcgc tggaaaacgt cgagcttccg gctctgctgc gcggtgagag tagcgcggaa
    16081 agtcgtaacg gggcgaaagc gttgctcgaa cagttagggc tgggtaaacg tctggatcat
    16141 cttccggcac agctttccgg cggtgaacag caacgagtgg cgctggcacg agcctttaat
    16201 ggtcgacctg atgtgctgtt tgccgacgaa cccaccggca accttgaccg ccagacgggc
    16261 gataaaattg ccgacctgct gttttccctc aaccgtgaac atggcaccac gttgattatg
    16321 gtgacccacg acctgcaact ggcggcacgc tgcgaccgct gcttacggct ggtgaacggg
    16381 cagttgcagg aggaagcatg attgcacgtt ggttctggcg cgaatggcgt tcgccgtcgc
    16441 tattaattgt ctggctggcg ctaagcctgg cggtggcctg cgtgctggcg ctgggcaata
    16501 tcagcgatcg catggagaag ggcttaagcc agcaaagccg tgagtttatg gcgggcgatc
    16561 gggcgttgcg cagttcacgc gaagtgccgc aagcgtggct ggaggaagcg caaaagcgcg
    16621 gcctgaaagt cggcaagcag ctgactttcg ccacaatgac ctttgcaggc gacacaccgc
    16681 agctggcgaa cgtcaaagcg gtggatgata tctacccgat gtatggcgat ctgcaaacta
    16741 atccccctgg cctgaaaccg caggcgggca gcgtattgct ggccccacgc ctgatggcac
    16801 tgcttaacct gaaaacgggc gacaccattg acgtgggcga tgccaccttg cggattgccg
    16861 gagaagtgat tcaggaaccg gattccggtt ttaacccctt ccagatggct ccgcgtctga
    16921 tgatgaatct ggcggatgtc gataaaaccg gagccgtgca gccggggagt cgggtcacct
    16981 ggcgttataa attcggcggc aacgagaacc agctcgacgg ctatgagaaa tggttgttac
    17041 ctcagcttaa acccgaacaa cgctggtacg gtctggaaca ggacgaaggc gcgctggggc
    17101 gatcgatgga acgctcgcaa cagttcctgc tgctttcggc gcttctgacc ttgctgctgg
    17161 cagtggcagc ggtggcggta gcgatgaatc attactgtcg cagtcgctac gatctggtgg
    17221 cgatcctcaa aacgctgggg gcagggcgag cgcaactgcg taagctaatc gtcggtcagt
    17281 ggttgatggt gctgacgctt tcagccgtta ccggtggggc cataggcctg ttgttcgaaa
    17341 acgtgttgat ggtgctgctc aagcccgttc tgcctgctgc actaccgcca gccagcctct
    17401 ggccgtggct gtgggcgctt ggcaccatga cggtcatctc gctgctggtg gggctacgac
    17461 cataccgctt gttgctggca acgcagcctt tacgcgtatt acgtaatgat gtggtagcga
    17521 acgtctggcc gctgaagttt tatctgccga ttgtcagtat ggtggttgtg ctgctgctcg
    17581 ccggattaat gggtggcagc atgctgcttt gggcggtgct ggcgggcgcg gtagtactgg
    17641 ctttgctgtg cggtgtgctg ggctggatgc agctgaatgt actttgccgc atgacgctga
    17701 aatcgctacc tctgcgcctg gcggttagcc gcctgttacg tcagccgtgg tcaacgttaa
    17761 gccagctttc ggcattttcg ctctccttta tgctgctggc gctgttgctg gtattgcgtt
    17821 gcgatctgct cgaccgctgg cagcagctac cgccagaaag cccgaactac tttttaatta
    17881 acatcgccac agaacaggta gcaccgctaa aagcgttcct cgcggaacat catatagtcc
    17941 cggaatcatt ttatccggta gtgcgggcgc ggctgacggc gattaacgat aagccgacag
    18001 aaggtaatga agatgaggcg cttaatcgcg aactcaatct tacctggcaa aatacgcggc
    18061 ccgatcataa tccgattgtc gccggtaact ggccgccaaa agccgatgaa gtgtcgatgg
    18121 aagaggggct ggcgaaacgc ttaaacgttg ccctcggtgg taccgtgact tttatgggcg
    18181 atacccagga gttccgcgct aaagtgacca gcctgcgcaa agtggactgg gaaagtctgc
    18241 ggcctaattt ctattttatt ttcccggaag gggcattaga cgggcaaccg cagagctggc
    18301 ttaccagttt ccgctgggag aatggcaacg gcatgttgac gcaactcaac cgccagttcc
    18361 cgaccattag cctgttagat attggcgcga ttttaaaaca ggtcggttag gtgctggagc
    18421 aggtaagtcg ggcgctggag gtgatggtgg tgctggtcac cgcctgcggt atgttgctgt
    18481 tgctggcaca ggtgcaggtg ggaatgcgcc agcgtcatca ggagctggtg gtatggcgca
    18541 cactcggtgc ggggaaaaaa ctgctgcgca cgacattgtg gtgtgagttc gccatgcttg
    18601 ggtttgtttc cggcctggtg gccgcaattg gtgcggaaac ggcactggca gtgttgcagg
    18661 cgaaagtgtt tgatttcccg tgggagccag actggcgatt gtggattgtt ctgccgtgca
    18721 gcggtgcgct gctgctgtcg cttttcggcg gctggctggg tgcgcgactg gttaagggta
    18781 aggcgctgtt caggcagttt gcggggtgat gagagtaaat aaggggcgtt ctggttgaat
    18841 ggaacgcctt gtttaaatta attctacaat atgtttcatt gatgatgtta ttgaattggt
    18901 gatttcctat ctttctattg ctgatattaa tctgaattgg tggagtttaa taattttaaa
    18961 tatagcccta caaaaataac tcagactaat aaaatcataa atcatatgcg ttgaatggat
    19021 attatccata tagtgaattt gttgatgatg aattcatctg tgctaaaaat tttagtttaa
    19081 taaaatattg aaagtgacct gtaataacag ttattgttga ttgagaacaa ataagtttat
    19141 gtgaaaaata tataaataca ttagctggtc atgtgtgtca ttttattttt ttgttgctaa
    19201 cacagggata tgaacaataa ctaaaagggc actttatatg agcggaaaac cagcggcgcg
    19261 tcagggagat atgactcagt atggcggtcc cattgtccag gggtcggcag gtgtaagaat
    19321 tggcgcgcca actggcgtgg cgtgctcggt gtgtccgggc gggatgacct cgggcaaccc
    19381 ggtgaacccg ctgctcggtg caaaagtaca gcccggcgag acggaccttg cgctgcccga
    19441 cccgctgccg ttcattctct cccgcaccta cagcagctac cggacgaaga cgcctgcgcc
    19501 agtgggaact ttttggcccc ggctggaaag cgccttctga tatccgctta cagatacgcg
    19561 atgatgcact ggtactcaac gacaacggcg ggcggagcat tcactttgag ccgctgctgc
    19621 cggggaaggc tgtgtacagc cgcagtgagt caatgtggct ggtgcgtggc ggtaaggcag
    19681 cgcagccgga cggccacacg ctggcacgac tgtgggcgtc gctgccgccg gatatccggt
    19741 taagcccgca tctttacctg gcgaccaaca gcgcacaggg gccgtggtgg atactggggt
    19801 gatctgagcg ggtgccgggt gctgaggacg tactgccagc gccgctgccg ccgtaccggg
    19861 tgcttaccgg gatggcggac cgcttcgggc ggacgctgac gtaccggcgt gaggccgcag
    19921 gcgacctggc cggggaaatc accggcgtga cggacggtgc cgggcgggag ttccgtctgg
    19981 tgctgaccac acaggcgcag caggcggaag aggcccggca gcaggcggtc gtcgccggag
    20041 caaaacgacc ggacattcct gattcactgc cggagagcac agagtacggc cccgacaggg
    20101 gtatccgcct ttcggcggtg tggctgatgc acgacccggc atacccggag agcctgcccg
    20161 gtgcgccact ggcgcggtac acgtatacgg aagccggtga actgctggcg gtatatgacc
    20221 gcagcaatac gcaggtgcgc gctttcacgt atgacgcgca gcacccgggc cggatggtgg
    20281 cgcaccgtta cgcgggaagg ccggagatgc gctaccgcta cgacgatacg gggcgggtgg
    20341 tggagcaact gaacccggca gggttaagct accgctatct ttatgagcag gaccgcatca
    20401 ccgtcaccga cagcctgaac cggcgtgagg tgctgcatac agaaggcggg gccgggctga
    20461 aacgggtggt gaaaaaagaa ctggcggacg gcagcgtcac gcgcagcggg tatgacgcgg
    20521 caggaaggct cacggcgcag acggacgcgg cgggacggcg gacagagtac ggtctgaatg
    20581 tggtgtccgg cgatatcacg gacatcacca caccggacgg gcgggagacg aaattttact
    20641 ataacgacgg gaaccagctg acggcggtgg tgtacccgga cgggctggag agcagccggg
    20701 aatatgatga accgggcagg ctggtatcgg agacatcgcg cagcggggag acagtacgct
    20761 accgctacga tgacgcgcac agtgagttac cggcgacgac aacggatgcg acgggcagca
    20821 cccggcagat gacggcggtt caccgcgagg aaggtatcag cctttaccgc cactatgaca
    20881 accgtggccg gttaacctcg gtgaaagacg cacagggccg tgaaacgcag tatgagtaca
    20941 acgccgcagg cgacctgact gccgttatca ccccggacgg caaccggagc gagacacagt
    21001 acgatgcgtg gggaaaggcg gtcagcacca cgcagggcgg gctgacgcgc agtatggagt
    21061 acgatgctgc cggacgtgtc atcagtctga ctaacgagaa cggcagtcac agcgacttca
    21121 gctacgatgc gctggaccgg ctggtacagc agggcggctt tgacgggcgg acgcaacgtt
    21181 atcattatga cctgaccgga aaactcacgc agagtgagga tgaggggctt gtcaccctct
    21241 ggcactacga tgcgtcggac cgcatcacgc accggacggt gaacggcgac ccggcagagc
    21301 agtggcagta tgacggccac ggctggctga gagaaatcag ccacctgagc gaaggccacc
    21361 gtgtcgctgt ccactacggc tacgacgata agggccgcct gacgggcgaa cggcagacgg
    21421 tggagaaccc ggagacgggg gaactgctgt ggcagcatga gacgaaacac gcctacaacg
    21481 aacaggggct ggcaaaccgc gtcacgccgg acagcctgcc gccggtggag tggctgacct
    21541 acggcagcgg ttacctggcg ggcatgaagc tgggcgggac gccgctggtc gagtatacgc
    21601 gggacaggct gcaccgtgag acggtgcgca gcttcggcag catggcaggc agtaatgccg
    21661 catatgaact gaccagcaca tacacccccg caggccagtt acagagccag cacctgaaca
    21721 gcctggtgta tgaccgtgac tacgggtgga atgacaacgg cgacctggtg cgcatcagcg
    21781 gtccgcgaca gacgcgggaa tacggctaca gcgccacggg caggctggag agcgtgcgca
    21841 ccctcgcacc ggacctggac atccgcatcc cgtatgcgac cgacccggcg ggaaaccggc
    21901 tgccggaccc ggagctacac ccggacagca cgctcacggc gtggccggat aaccgcatcg
    21961 cagaggatgc gcactatgtt taccactatg atgaatacgg cagactgacg gagaagacgg
    22021 accgcatccc ggcgggtgtg atacggacgg acgacgagcg gacccaccac taccactacg
    22081 acagccagca ccgcctggtg ttccacacgc ggatacagca tggcgagcca ctggtcgaga
    22141 gccgctacct ctacgacccg ctgagccgcc ggacggggaa acgggtgtgg cgtcgcgagc
    22201 gtgacctgac ggggtggatg tcgctgtcgc gtaaaccgga ggtgacgtgg tacggatggg
    22261 acggcgaccg gctgacgacg gtacagaccg acaccacgcg tatccagacg gtataccagc
    22321 cggggagctt cgcgccgctc atccgcatcg aaacagacaa cggtgaacgg gagaaagcgc
    22381 agtgccgcag cctggcggag aagttacagc aggaagggag cgaagacggt cacggtgtgg
    22441 tgttcccggc tgaactggtg gggctgctgg acaggctgga gggggaaatc cgggcaaact
    22501 gcgtgagcag tgaaagccgc cagtggcttg cgcagtgcgg gctgacggtg gagcggctgg
    22561 cggcacagat agagccggtt tatttgcctg agcgaaaaat acatctttat cactgtgacc
    22621 accggggcct gccgctggcg cttatcagtg aagacggcaa tacggcgtgg agcgcggaat
    22681 atgatgaatg gggcaaccag cttaatgagg agaacccgca tcacctgcac cagccgtacc
    22741 ggctgccggg gcagcagtat gataaggagt cggggctgta ctacaaccgg aaccggtact
    22801 acgatccgtt gcaggggcgg tatatcactc aggacccgat agggctggag gggggatgga
    22861 gtctgtatgc gtatccgctg aatccggtga atggtattga tccattaggg ttaagtcccg
    22921 cagatgtagc gctaataaga agaaaagatc aacta
//
LOCUS       DAJVUJ010000054        22864 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.054, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000054 DAJVUJ010000000
VERSION     DAJVUJ010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22864
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.054"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            39..968
                     /gene="aaeR"
                     /locus_tag="OQQ48_001918"
     CDS             39..968
                     /gene="aaeR"
                     /locus_tag="OQQ48_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HCS1952710.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            complement(1096..2541)
                     /gene="tldD"
                     /locus_tag="OQQ48_001919"
     CDS             complement(1096..2541)
                     /gene="tldD"
                     /locus_tag="OQQ48_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HCS1952711.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVGHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVRGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(2697..6497)
                     /gene="yhdP"
                     /locus_tag="OQQ48_001920"
     CDS             complement(2697..6497)
                     /gene="yhdP"
                     /locus_tag="OQQ48_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HCS1952712.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGGNDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFHLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDDVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKLLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPAAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLPVNVKVDGNLSSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDLTISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            complement(6565..8034)
                     /gene="rng"
                     /locus_tag="OQQ48_001921"
     CDS             complement(6565..8034)
                     /gene="rng"
                     /locus_tag="OQQ48_001921"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HCS1952713.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            complement(8024..8617)
                     /gene="yhdE"
                     /locus_tag="OQQ48_001922"
     CDS             complement(8024..8617)
                     /gene="yhdE"
                     /locus_tag="OQQ48_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HCS1952714.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAHKKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVVLADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            complement(8626..9114)
                     /gene="mreD"
                     /locus_tag="OQQ48_001923"
     CDS             complement(8626..9114)
                     /gene="mreD"
                     /locus_tag="OQQ48_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCS1952715.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            complement(9114..10199)
                     /gene="mreC"
                     /locus_tag="OQQ48_001924"
     CDS             complement(9114..10199)
                     /gene="mreC"
                     /locus_tag="OQQ48_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCS1952716.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPTTGIAQPTPQQPTTGNAATA
                     PAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            complement(10265..11308)
                     /gene="mreB"
                     /locus_tag="OQQ48_001925"
     CDS             complement(10265..11308)
                     /gene="mreB"
                     /locus_tag="OQQ48_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HCS1952717.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNN
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            complement(11613..13553)
                     /gene="csrD"
                     /locus_tag="OQQ48_001926"
     CDS             complement(11613..13553)
                     /gene="csrD"
                     /locus_tag="OQQ48_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HCS1952718.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSD
                     LFRELSVPLIKYPGMSLRLVYQDPMGNYFHSLMTTAPLTGAISFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            13705..14679
                     /locus_tag="OQQ48_001927"
     CDS             13705..14679
                     /locus_tag="OQQ48_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCS1952719.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVMVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAIIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            15657..16127
                     /gene="accB"
                     /locus_tag="OQQ48_001928"
                     /gene_synonym="fabE"
     CDS             15657..16127
                     /gene="accB"
                     /locus_tag="OQQ48_001928"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCS1952720.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            16138..17487
                     /gene="accC"
                     /locus_tag="OQQ48_001929"
     CDS             16138..17487
                     /gene="accC"
                     /locus_tag="OQQ48_001929"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCS1952721.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(17579..18626)
                     /locus_tag="OQQ48_001930"
                     /pseudo
     CDS             complement(17579..18626)
                     /locus_tag="OQQ48_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787050.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
     gene            complement(18623..19585)
                     /locus_tag="OQQ48_001931"
     CDS             complement(18623..19585)
                     /locus_tag="OQQ48_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCS1952722.1"
                     /translation="MSKVFTIGEILVEIMASKIGQPFDQPGIWNGPYPSGAPAIFIDQ
                     VTRLGVPCGIISCVGNDGFGDINIHRLAADGVDIRGISVLPQEATGSAFVTYHNSGDR
                     DFIFNIKNAACGKLSAQHVDENILKDCTHFHIMGSSLFSFHMVDAVKKAVTIVKANGG
                     VISFDPNIRKEMLDIPEMRDALHFVLELTDIYMPSEGEVLLLSPHSTPERAIAGFLEE
                     GVKEVIVKRGNQGASYYSANEQFHVESYPVEEVDPTGAGDCFGGAWIACRQLGFDVHR
                     ALQYANACGALAVTRRGPMEGTSRLAEIEAFIQRHDMSIREAAQ"
     gene            complement(19607..20596)
                     /locus_tag="OQQ48_001932"
                     /pseudo
     CDS             complement(19607..20596)
                     /locus_tag="OQQ48_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525780.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(20597..22096)
                     /locus_tag="OQQ48_001933"
     CDS             complement(20597..22096)
                     /locus_tag="OQQ48_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCS1952723.1"
                     /translation="MTDVILDVSHIAKTFGHVQALKDITLSLRKGRVHTLLGENGAGK
                     STLMKILAGVYPPTQGTITLRGETITINNPQHSRQLGIAIIFQELSLSNNMTVAENIY
                     ANNEPRRFGIINDKKMLADCQNLLAERGIPLDPLEMVGNMSMAHRQLVEIAKALSYAA
                     DVVIMDEPTSSLSDNEAEILFNIIEKLKQRGCAVIYISHRMDEIMRISDDISVIRDGE
                     YIATHEKKNSDIQHLIAQMVGREMKNIWPARLGEIPDENVPAKLEVKNLSHPSLFKEI
                     SFAVRPGEVLGFFGLVGAGRSDVMKALFGLVSYQGTVLIDGKEVRITNPKQAIDHGIA
                     FVTENRKEEGLVLMHDVNMNTHHVAFQYNASRMGLINHRQEEAKTLQSIARMNTKVSS
                     VHQAVGALSGGNQQKIVLSKWLEKTPRILLLDEPTRGVDVGAKFEIYNVIRQLAAAGT
                     AIILVSSELPEVMALSDRLVVMRNKTIADIYSCENLTQTQVMTAATGVR"
     gene            complement(22157..>22864)
                     /locus_tag="OQQ48_001934"
     CDS             complement(22157..>22864)
                     /locus_tag="OQQ48_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCS1952724.1"
                     /translation="LFANQDLNKQLSDVEDFITKKVDAIIISPVDSKGVQAAIIKADK
                     AGIPVITVDVAAEGVPVVSHVATDNYAGGVEAGKLMGKLLNGKGTVAIISYPALQSVV
                     ARVDGFKKGLSDTPDIKIVAEQPSITRAEALTTAQNIMQANANLNGLFGFGDDAALAA
                     VIAAKSAHNDNIKIIGFDGMKEARDAVDSEKTFAAVIRQYPDQMGAKAIDAAVDHLNG
                     KPVEKMIPVQPGVYTGK"
BASE COUNT         5669 a         6193 c         5459 g         5543 t
ORIGIN      
        1 attatgtgat ctaaatcact tttaagtcag agtgaataat ggaacgacta aaacgcatgt
       61 cggtgtttgc caaagtagtt gaatttggct cttttaccgc cgccgccaga cagctacaga
      121 tgagcgtttc gtccatcagt cagacggtgt caaaactgga agatgagttg caggtaaagc
      181 tgttaaaccg tagcacacgc agcattggcc tgaccgaagc cggtagaatt tactaccagg
      241 gctgccgtcg tatgcttcat gaagtgcagg atgttcatga gcaactgtat gccttcaata
      301 acacccccat cgggacgcta cgcattggct gttcttcaac tatggcacaa aatgttctcg
      361 ccgggctgac agccaaaatg ctgaaagaat acccaggttt gagcgtcaat ctggttaccg
      421 gaattccagc ccccgacctg attgccgacg gtctggatgt ggtgatccgc gtcggcgcgt
      481 tgcaggattc cagcctgttt tcccgccgtc ttggcgcgat gccaatggtg gtgtgcgccg
      541 cgaaaagcta tctcacacaa tacggcatac cggaaaaacc cgccgattta agtagccatt
      601 catggcttga atacagcgtg cggcccgaca atgaatttga actgattgca ccggaaggga
      661 tctcgactcg cctgatcccg caagggagat ttgtgactaa tgatccgatg acgctggtgc
      721 gctggctaac ggcgggtgcc gggatcgcct acgtgccgct gatgtgggtg atcaacgaga
      781 tcaatcgtgg ggagctggag atcctgctgc cgcgttatca gtcagatcca cgcccggttt
      841 atgcgttata taccgaaaaa gataagctgc cgctgaaggt acaggtcgtg atcaactcgc
      901 tgacggatta ttttgttgag gtcggtaaat tgtttcagga gatgcacggg cgcgggaaag
      961 agaagtaatt catattgtac tgttacgttc tacaaacctg tgccaacggg ttcccctcac
     1021 cctaacccca gaggagcgag gggaccgttc gtgcacgtag aaagattaat tatccttctg
     1081 aaaataatga aattattacg cagtaccgcc aacagtcagg ttatcaactt tcagcgttgg
     1141 ctggcccacg ccaaccggca aactttgccc ttctttaccg cagacaccca cgccgttatc
     1201 cagtttcagg tcgttgccaa ccatcgaaat ctgctgcatg gtttcgatac cggaaccaat
     1261 caacgttgcg cctctcaccg gcttcgttac tttaccgttt tcaatcagat atgcttctga
     1321 agtggagaaa acgaatttgc cggaggtgat atccacctga ccgccaccaa agttcggtgc
     1381 atagataccg tactcaacgg attcaataat ttcctgcggg gtcgatttac ccggcagcat
     1441 ataggtgttg gtcatacgcg gcatgggcag atgggcgtag gattcacggc gaccgttgcc
     1501 agtcggcgtc atccccatca aacgcgcgtt gagtttatcc tgcatgtagc ctttcagaat
     1561 gccgttctca atcagcacgt tgtactggcc tggcgtacct tcgtcatcaa tcgccaccga
     1621 accacggcga tcgaccatcg tgccgtcatc aaccacggta cacagttctg aagccaccag
     1681 ctccccgacc tgcccactaa atactgaagt gccgcggcgg ttgaagtcgc cttccagacc
     1741 gtgacctacc gcttcatgca acagcacgcc cggccaacct gcgccaagta ctaccggcat
     1801 ggtgcccgct ggtgcagcaa cggcagaaag attgaccagc gccatacgca ctgcttcttt
     1861 tgcccatgca tccgcacgga cttcgccgtc aagatcggca aggaagaact cataaccaaa
     1921 acgaccgcca ccgccactgg caccgcgttc gcgtttgcca tcttcttcga ccagaacgct
     1981 cacggaaaga cgcaccagcg gacggacatc cgccgctagc gtgccgtcgg tggccgcaac
     2041 cagaattaat tcatagacac cactgaggct ggcagtcact tcctgtacgc gcttgtccgc
     2101 ttcgcgagca accttatcga cgcgacgcag gatatccagc ttctcttcac ggctcatgct
     2161 ttgcagcgga tctaccgagg tatacaacgg gctatgccct accgcgccca gcgtctgtac
     2221 tttgccatca ccactatcac ggacgatggt gcgcgccgct tgcgcactct gttccagcgc
     2281 cagcaggctg atttggtcag cgtaagcaaa tccggttttt tcaccgctga ttgcacgcac
     2341 gccaacgccc tgatcgatgt tgtaagaacc atctttaata atgcggtctt ctaaaaccca
     2401 ggattcgtga tagctcgact gaaaatagag atcgccataa tcaaggcgac gttcggccag
     2461 ttgaccgagg atcgcgaaca agtcctgatg tttcaggccg ttcgccgcta gcaattgttc
     2521 acttactagg ttaagactca tcgtttttgc tactcgttag ttactgcagt agaggatttt
     2581 ttacggctgc cggatgcggc gtgaacgccc tatccgacct acggttatgt tctggctcgt
     2641 tggcctttgg caacgattat catatgagat tggggcaatt acgcgccctc gtcaaatcat
     2701 tgcgcttttt ctttacgcgg ttggcgcaac acttcgttga tttgcggatc gtccagcgga
     2761 cccgaaatgt gatagcgcaa aatggagact ttgctccaca gcggccccag cactttactg
     2821 gctgcaaata ctgccgcgcc aacaatgggg ttaaccgcaa aagccgcagc cacgcccacc
     2881 gtcgcagaaa tctctggtgc gacaaccgct tccatattca ggtcgcgacg taccagattt
     2941 accgaccctt tcatggcgat atccgcctcc aggccatcca ccagcgtgtc gtcggtgtgc
     3001 ataacgccgt ctttaatcca cgcggtgctg cgaatggagt caaaatagaa cccttcgcca
     3061 aaagtgtctc tgaaatcaaa acgcagctta cgcatcaggg catcaacgct caataagcgc
     3121 agcaattgcc cggcatgtcc ggtattgatt tcggtaattt cgcctttacc cagttgagta
     3181 tgaatgatgc cattcaacgt cgcctcatct ggttgccagg gtgctttgcg ccagtgtaaa
     3241 tcgtaatcca cattaaatga cgactggcgt atgggcgtcg tgacaccaaa aaattctgcg
     3301 gcggcatcaa ttttctgccc gcgcagtttt cctttcagcg aggtacgttc attccccgga
     3361 ttattaaccc attcaccatc ggcagtaagc cgcgagaaac cagtatcaat cagtccattg
     3421 gtcagcgtta acgtatcgcc agaaatggtg agatcactgt caatacgacc gaatttttgc
     3481 ccccagaacc agcactctgt gcatcgtatt tgtgcgtccg gccagccacg gaagttaatg
     3541 cgctccgttg tcgggaaagg tgatgacggt gttgaatcac cacgagtttt cgccacgctc
     3601 gggttgtaat aaagatattt gatattcgcc agccacggcg cgttattacg catcgccagc
     3661 gtggcattga tttcacgccc ctgtgcctca accagggtgc catttgccgt cggttgcgaa
     3721 acaatactca ggttattcca ttgctgattt cccagtgaca acataggcgt acgtaacgtt
     3781 atgtgttgtg ggaaactcgc tgcaccaccg acactctccg cagcgccttt ctggaacagg
     3841 gccagccact cggcaccatt catcggcggc atattgagtt caacgccact ttgttccggc
     3901 aacggcggga gcgttttact gtctgccgcc caaatagcac gatcgagcgt cagcttttga
     3961 ccaagcaacc agcggctatt gaaatggtta tccgcaccag cctgtccggt taattcaaag
     4021 ctgctgagat tgccatcaac cttaacgttt accggcagtg gttcacccgc aggtttggct
     4081 aacggtgaag gtaagtgact gctcacatta ttcagatcgc cgttcagctc tacgttatag
     4141 gtcgcgccag catgataagg cagatcaatg cccactttac catcccacgc cacgctgcca
     4201 ctcaatgctt cgttcaccgc tgcaggcaga acgccggttt tcgccggttg ccagttaccg
     4261 ttgaggttta ccgcaacctg gtaggctttt gccccttctt tggtggaaaa atccacgttc
     4321 aacggctgat taaaccagct tgctgtcagt ggttcacttt gcagatcgcc attgataaag
     4381 ctgaatttac cgctcaaatt tttcagggtg ctgtcgagta gtttgataaa cagactgtta
     4441 ttacgcagcg tcacttcacc tttcgcggtt accagttcgc cgttcagcgg gatatcaaga
     4501 tgtaagcgag cattcacatc gccgtcgagc tggagttctt gcagggtcgc acccagagaa
     4561 tctttcagcg gtgtctcatc aaagtaaggg ccaacggctt tacccggacc tttaatgtca
     4621 gcgtcaatca gcagtttttc tttcgagtag tcagggatca ctgcggtgag attactcgcg
     4681 cgcacgccgc ccagattaac gtcatcggtt ttcatccata aaccgtcgtt aataaagtcc
     4741 agttcaatat caaggttagt taatgcaggc cagtccggct ggaaggcaaa cttcgcgtta
     4801 cgcagcggca ccagcacttc aaactgacct tcgttgtgtt tataggggaa gagttgcgga
     4861 ttgccaccat aaaccagcgt cgcgttatcc gcttcaccgc cctgaatcgc gccacttaag
     4921 taatcaacta ggtctttacc catcaagttt tccgggaaat agcgccaggc ttgtgaacca
     4981 tcatcggtac tgatgccagc cagaataccc agccagggtt catcgttagc aggttgcagg
     5041 taacgaaaac cgccgcgcgc atggacagct ttggctttaa cgtcaatatt acgcccatcc
     5101 aggtggaaac ctttgtcatt gttcagccag cttatagttg cctggccgtc ggcgatttcc
     5161 agtggcgcac ggaataccgt ttcgtaaggc atctttgcct gcttcatcga cgcggtaagc
     5221 aaaccatttt caacgctgcc ggaaagcgtc ccggagaagt gttccgcacc cggtaataat
     5281 ttccattgct tccaggccag atcgctccac gatgcctgaa aacgggtctt gtctgccgcc
     5341 tgaagcggga tatccagcgc cagagtgtta atcttgccgc tcggttgtgt ggagcgccaa
     5401 acatcaccca gtgcaggtga aagtttcgcg gccagcgggc gtatgccctc caggcctgcc
     5461 agctccagat tactggcgcg aatccggagt tcgtcactgc gtttattgtc tttgccgcca
     5521 acgtcctgtt ccggtatcca ggccagcgtc aatgctccgc tcggccaggg tttgccgtcc
     5581 atcgtgatcc gtgtatcggg aatagagaac tgccagcccg gattttcacg cgtaatatgc
     5641 gcggtcagat tatccaccga cagcgtatgc gtttgcttct cgcccaacca gctggcaccg
     5701 ccctgtttca gccagacgtc accgccggtt acatcgcctt tgtcgatcgt catccagcct
     5761 tcaagggaga actgtgccgt ttccagagca atattgtcct gcatccattt accgagccac
     5821 ggcttcaggt cgatgtcatc cgcctgtagc cagacgcgac cattgcttaa caacccctca
     5881 tcatcgcgca aatccatgcg cacctgcatc acgccgtgct gtccggtaag gctggagagg
     5941 cttaccaggc cttccgcacg gtgtcgacgt ggatcgttca gccaggtgag ttgtgggatc
     6001 gccagctcgg cgcgctgacc ggatggcgtc agaaaactga cttcactgtc gcggagatcg
     6061 aaatggtcaa attgacgaag aaacagatcg ctgatgtgac tggcttccag gctatcatta
     6121 ccaccaccgc tggtgatagg agtgttggtg cgaaagcgca gctgccagaa agtgaggtcg
     6181 cgaaactgcc agcgcatatg taacaagctc tgccagacat ccagcgccag agtaacgcgt
     6241 ttaaccgaaa attcgccgcc atcttttagt tctgcacgga tgtcgtgtgc ttcaagcgtc
     6301 gggccaaaat tctgccagct ggctgagagc tgactggctt ctaccggcat gccagtcgcg
     6361 gattctattt tgttgaggat ttccggacgc caggcgtcaa gatgcggtaa agcaatacgc
     6421 aggccgctaa ccagcagggc tgcgatcaca acgagcgcgg ctccagtaag cagtaaaatc
     6481 cccggcaatc gcctcacccg tcactccttg tctgctaaaa atgtgactca aaaacccttt
     6541 gccggatggc ggcccagcat ctgtttacat cattacgacg tcaaactgct cctggttata
     6601 gagcggttca atttgtactt taacctgttt gccaacgaaa atttccactt ccgccagcga
     6661 gtgtgactct tcgcctttca aggcttcagc tactgccgga gaagcataga ccaggaaacg
     6721 gtcggagtcg taagcatggt ggacacgaac aatctcgcgc atgatttcat agcataccgt
     6781 ttccacggtt ttcaccgttc cgcgaccgtg gcaggttggg cattcgttac acagtacgtg
     6841 ctcaatgctt tcgcgggtgc gtttacgcgt catctccacc agccccagcg ccgaaaaacc
     6901 attaacgctg gttttcaccc ggtctttgct caacgcctgc tccagcgagt gcagcacgcg
     6961 gcggcggtga tcttcattat tcatatcgat gaaatcaata atgataatcc cacccagatt
     7021 acgcaaccgt aactggcgag cgatagcctg cgtcgcttca atattggtat tgaaaatggt
     7081 gtcgtccaga ttgcgatgac cgacaaacgc tccggtattg atgtccacgg tggtcatcgc
     7141 ttcggtctgg tcgataatca gataaccacc ggatttcagt tctactttgc gttccagcgc
     7201 tcgctggatt tcgttttcga catcaaagag atcgaaaatc ggctggcgtc cggtgtaatg
     7261 ctccagcttg ctggtcatct cgggaatgta ctccgaggta aactccagca atgcttcgta
     7321 agtcaggcgt gagtcaacgc gaatgcggtc cagttcggcg tcggcgaaat cacgcagaac
     7381 acgctgcgcc agcgccagtt cgccgtacag ctgataacgg gtctgcgggc gttttttacg
     7441 ctccataact ttggtccaga cgcgtttcag ataagcggca tcggaggcca gttcagcctc
     7501 gccaacccct tccgctgcgg tacggatgat aaacccgccc tgctcgtcgc aatactctgc
     7561 gaccactttt ttcaggcgtt cacgttctga ttcgctttca atacgttggg aaaccccaac
     7621 gtgagaagcc cctggcataa acaccagata gcgagaaggc agcgtgatat cggtggtcag
     7681 gcgcgcacct ttagtgccaa gcggatcttt caccacctgc accatcagat cttgcccctg
     7741 acgaaccagt tccgagatgt cgcgcaccgt gaattgcttt tgttcttcac ccgccacaca
     7801 ttcggtgtgc ggcatgatat cggatgcatg aagaaacgcg gctttatcca gcccaatatc
     7861 tacaaaagcc gcctgcatac ccggaagtac acgacttaca cgacccttgt agatattgcc
     7921 tactattccg cgtcgcgcct cacgttcaat atgaatttcc tgcagaatac cgccatcaat
     7981 atacgccact cgcgtttccg aaggcgttac gtttactaac aattcagccg tcatgtttat
     8041 cccttttctc acgcagtgcg ttaaaattac ttaataattc atacgtttca accagcggta
     8101 agccgactac ggcgtgatag ctgccattta tcttcctgac aaaacagcca cccagcccct
     8161 gaataccgta tgcacctgct ttatctaacg gttcaccgct ggcgacatag cccgcgatgt
     8221 cttcgtctgt taacgttctg aaagtcacat cggtgaccac caggcaatcg agaatgtgct
     8281 ggctgtcggc caacaccact gccgtcatca cctgatgggt ctgacccgat aatttgcgca
     8341 acatctgcgc cgcatgctct gcgtcgcgcg gtttctccag cacttctcca ttcaggataa
     8401 cgatagtatc cgcacccagc accgggagat cctgcgccgt ttgcgcgaca cccgcctgtg
     8461 ctttcttgtg tgccagacgc acaacatact gctgcgcgct ctcctgcggc tgacgctgct
     8521 cctcaatgcc cgtaacaata cgttcaaagg tcacgccaag ttgcgcaagt aactcctgac
     8581 gacgcggaga accggaagct aaatacagag aagtcataga aacctttatt gcactgcaaa
     8641 ctgctgacgg actttacgca tcagcaagaa aatccacggc cagagcaccc cattgactac
     8701 actactccag aacacttccg gtctgaaaga gacgttaatc actaaaaact ctgcccagaa
     8761 aacaataata tccaccacca gcgaaagcaa catgacgact agcgcctgct gccataatgc
     8821 gaggttgcgg aaaagctggt atttcagcgc caccaggtaa gcaatgatgc tcatcgccaa
     8881 tacgcgtacg ccaagcgtcg agccgctgat cagatccagt atggcaccca tcacaaaacc
     8941 tgtgcccaca tttacgcgat ggggcaaggc caggatccaa tacaacaaga taagtaacac
     9001 ccagtttggc cggaaaacaa tcaggttatc cggccagggc atgatttgca gcaacagcgc
     9061 aatgaggaaa gagagccaga ttacccaacg tccctggcta cgatagctcg ccactattgc
     9121 cctcccggcg cacgcgcagg cggttgagca ccactttgcg gcggcgtagc cctttgtgga
     9181 gagcgattag cagcaggcgc agtagctgca tttcctgtcg tcggttgctg cggagtcggc
     9241 tgagcgatcc ccgttgtcgg ttcaggtaac tttggcccca tcgcgtctgg cgaaggcaat
     9301 acctgcggca tcatctgcat cagacgttca ttagcaacac gatgcacctc ttccggcgtc
     9361 atcgggttag cgccgttacg atccgcgccc cacagcagca gcagataacg caaacgttgc
     9421 agtcctgcag tcggacgcgc ctgaatcaca gtataagcgc gctgggtatc gagttttacg
     9481 gaagagacaa ccgcgaccgg atagccttcc gggaaacgac cgcccagacc ggaggtcacc
     9541 agcacatcac caacacgaat atccgtattc gctggcagat gctcaagctg caaatcatct
     9601 gtacaaccgt taccggctgc aattacgcgg atatcgttgc gcagcacctg gattggcagc
     9661 gcgtgggtcg catcacaaat cagcagcacg cgactggtca gtttagcgac ggccaccacc
     9721 tgaccaacaa cacctttgtc gctgatgacc ggctggcctt cataaacgcc attaacgcta
     9781 cctttatcga taacaacttg atcgctataa ggatcgttaa ccgtggagat aacctgagtc
     9841 accattttct gctcatcctg acgcagcggg gaacccagca gctcgcgcag acgcgcgttc
     9901 tcctgtttgt attgtccaag catcagcagt tcactgtttt tcagcaacag ttcctgacgt
     9961 aacgcccggt tttcaagttc tagttggtca cgcgaggcca gcgtctgcga tacgccatcc
    10021 agcaattcac gaggagcatt ggaaacaaag tagaaaggac tgacggcggt atccatataa
    10081 gtacggattt gactgaacgt ccccaggcgg ctgtcggcaa taataatgcc gagcgccacc
    10141 agcaccgcca gaataaggcg aatctgtagc gacgggccac ggctaaaaat tggcttcata
    10201 agttatgcgt attctcgtat cagaccaggc agggtaaaca gacatttccc ctgcctgcat
    10261 ccgattactc ttcgctgaac aggtcgccgc cgtgcatgtc gatcatttcc agcgctttgc
    10321 cgccaccgcg cgccacacag gtcagcgggt cttcagcaac aacgactgga atgccggttt
    10381 cttccattaa caaacggtca aggttacgca gcagtgcgcc accaccggtg agcaccatgc
    10441 cgcgctcgga gatgtcggaa gccagttccg gcgggcactg ttccagtgca accattaccg
    10501 cgctcacaat accggtcagc ggttcctgca gtgcttcgag gatttcgttg gagttcaggg
    10561 taaaaccgcg tggaacacct tctgccaggt tacggccacg aacttcgatt tcacggactt
    10621 catcgcccgg gtaagccgaa ccgatttcgt gcttgatacg ttctgcggtg gcttcaccga
    10681 tcagagaacc gtaattacga cgcacatagt tgatgatagc ttcgtcgaaa cgatcaccac
    10741 caatgcgcac ggaagaggag taaaccacgc cgttcaagga gataacagca acttcagtgg
    10801 taccaccacc gatatcaacc accatagaac cggtcgcttc agaaaccggc aggccagcac
    10861 caattgcggc agccatcggt tcttcaatca ggaagacttc acgggcacca gcgccctgcg
    10921 cggattcacg aattgcgcgg cgttcaacct gggtcgcgcc aaccggtaca caaaccagaa
    10981 cgcgcgggct tggacgcata aagttgttgc tgtgcacttg tttgatgaag tgctggagca
    11041 ttttttcagt cacgaagaag tcggcgataa cgccgtcttt cattgggcga atggcagcaa
    11101 tattgcccgg cgtacggccc agcatctgct tcgcgtcatg acctactgca gctacgcttt
    11161 tcggtgaacc ggcacgatcc tgacgaatgg ccaccacgga aggctcattc aatacgatgc
    11221 cttgtccttt tacataaatg agggtattcg cagtacccag gtcaatggac aagtcattgg
    11281 aaaacatgcc acgaaatttt ttcaacatac taagggataa tcctgaaagc tggggcggaa
    11341 aagaaaatcc gcttacttta ccaaccacac gcagcagcga caaggcgcaa aaatcatctg
    11401 ctacggtgaa aattagtgca gttcgtttcc tttgttacaa atctctgcct gagtccagaa
    11461 aggcttaatg catcagcagc attcctcgcc tgtttgcaac cgcgtaaggt cattcatctg
    11521 catatgtgct gcaacaatct ggcgagcaga caagcacact cccatgagac gcagcgcgcg
    11581 ttattctacg tgaaaacgga ttaaacggca ggttaaaccg agtatctttg tgaatatttt
    11641 ttcacgttag tatcaagtgg ctgtgaggac gcgaaaaaat ccccttgccc gcctgtaaca
    11701 ccgcgctgaa tcagggtctg ccactcgctt cgcgaacgca caccggtggc gtaaacctgg
    11761 gtgctggtcc cggagcaggc ttccaccagg ctttgaacca gcagctggtt ctccgttcgc
    11821 ttctcaatgt ttctgaccag ccccggatgg agcttgagta actcaacatt aagttctttg
    11881 atccaactgg tgcttaccag cgtcaaacca gcctggttga cggctacccg tacccctaaa
    11941 gcattcacta aacgaataac aggttgtaaa cgactgatat gttgacctac atcggcctct
    12001 gcaagttcaa taattatgcg ttttcgttgt gatttttcac attgcattaa cgtatcgcgc
    12061 agccaacgct gaaaacgcgg gcgaatcagc gactcaacgg taacctgaat cgccagattt
    12121 tcctctggcc agtaacgcaa tagtggaata agacggctga tttgcagacg gtcatactct
    12181 tccgataagc caaactgcaa gaccatcggc atatactccg ccgagctaac ctcttcatta
    12241 ccatcgaaga tgcggcacat gagttcgcga tgatgaacct gaccttcgcg agtaaccgcc
    12301 ggtttttgat aaaggcgcgg gccgccgcga ctgagcattt gctcgataag cgtacgccag
    12361 cgaacattac cgcgtccttt ttcaggcaac gagtcatcgt aaatagccca gctattgccg
    12421 ccctgcaatc ccgcattacg cgtggcagac tctgcatgtt ccattacctg ctcggtatcc
    12481 tgaccactac gccaggcgca gataccaatg tggatcatat cgtcgcgatc gagcatttta
    12541 ttgttcggca aggtatcgac ggctttgatt aactgaccgg cgatgctctc tgcctctttt
    12601 aacgtccggt gcggtaacag cgcagcaaaa tcactgcggt ggtaacgcgc cagcagtgcg
    12661 ccagggtagc gcatcataaa tgtcgacagc agattcgtca gagtgaagaa ctgttcttca
    12721 acctggctgt gcccccaagt atcgctcaac atattgaaat ccggcagacg aatcatcatc
    12781 acgatcccgt gggtacctac tttctcctga tcttccagta acgttgctaa ctgattatcg
    12841 aaaaagagtc ggttattgag gccggttttc acgtcctggg cggcataaga gcggatcagc
    12901 gtatcaagac ggctgtgttg ttcgcgtgcg ttctgaattt cacgaagcag cgtatccagc
    12961 gcactgctgg ttctgggcgg ccattcatag atggttccca acacattaga gccacgctca
    13021 ccgtttaaga tacgagtagc ccgggtttcc agcaattctt gcccggcaag ttgccgttgt
    13081 aaccagcgta ccgccaagaa gagcataaca atgataaagc taatcgcccc cgtgagcggc
    13141 gcggtggtca tcaacgaatg gaaatagttg cccatcggat cctgataaac cagacgcagc
    13201 gacatccccg gatactttat caacggaacg ctcagttcac gaaacagatc gctggagcca
    13261 accggacgat aactaccatt tctggccagg gtataaacct gtttatcacc atggagcagg
    13321 tctacacgaa cgatatctgc cgacatcatt aattcggtaa tttgtggcct taatgtgctg
    13381 aagtcattcg acacaaggtg ggtatcaatc gccgtcgcca ccgcctgaac gcgatgacta
    13441 aacttatact gaattgcgtt gtagaaactt agcgaacagc ccagcaaagt cacaaaaatt
    13501 gttaacccgg tgagcagcgt aacaaaggcc gaaaatttcg tcgttaatct catccttgtg
    13561 ttaactccga tagtgaggaa gcgggcatac tagcaaatca gatttatctc gcaatttatt
    13621 gcgcttcatc ggctttgctt ttccattagc gagtataatc ttcagaaatt attttccaat
    13681 ccatcatgca catgaggacc acttatgcag gcgttacttt tagaacagca ggacggcaaa
    13741 actctcgcat cagtacagac tctggacgaa agtcgcctgc cggagggcga tgtcatggtc
    13801 gatgttcact ggtcgagcct gaactataaa gatgcgctgg cgattaccgg taagggaaaa
    13861 atcatccgta attttccgat gattcctggg atcgattttg ccggaactgt acgcaccagc
    13921 gaagatccgc gttttcatgc cggtcaggag gtgttactca ctggctgggg cgttggtgaa
    13981 aaccactggg gtgggctggc ggagcaggcg cgagtgaaag gtgactggct ggttgccatg
    14041 ccgcaagggc tggacgcgcg taaagcaata attatcggta ctgccggttt taccgccatg
    14101 ctgtgtgtga tggcgctgga agatgccggt gttcgcccgc aggacgggga gattgtcgtg
    14161 acgggtgcca gtggtggcgt cggcagtacc gccgtggcgc tgctgcataa gttgggttat
    14221 caggtcgttg ccgtttccgg tcgcgaaagt acccatgaat atctgaaaag tttaggtgcc
    14281 agccgtattc tccctcgtga tgagtttgcc gaatcccgtc ctctggaaaa acaagtctgg
    14341 gctggggcaa ttgacactgt tggcgacaaa gtgctggcaa aagtgctggc gcaaatgaat
    14401 tacggcggct gcgtggcggc ctgtggtctg gcgggtggtt ttactctgcc aaccacggtc
    14461 atgccattta ttctgcgtaa tgtccgtttg caaggggtgg attcagtaat gacgccacca
    14521 gaacgccgcg cacaagcctg gcagcgactg gtcgccgatt taccggaatc attctatacc
    14581 caggcggcaa aagagatatc tctgtcagag gcaccgaact ttgccgaggc catcattaat
    14641 aaccagatcc agggtcgcac gctggtgaag gttaactaac catttagcaa ggaataataa
    14701 gagagggaac tcattttgac atcattattc aatcggttaa cgggaaaagc ggttagccgg
    14761 acagctttcg tcgaacacct tggtcaggaa gttgtacaac atcatccaaa ctggaaagtc
    14821 atgatttcga ctgaccacaa attgatgcgc attgatactc ctctaaacag ccattattga
    14881 tacgcctccg tcgcctgtta ggtttatggt gctttgcctg ggcgacacta cgcttaacct
    14941 gttactgatt tctggtacca tggggtgaat aatctgattt tatttgacta caaattaacc
    15001 actcgaacct atttaatgct gagaattgtc agtcggttga ttttgcgtgc tttagcattc
    15061 acatctatcc agacgatgca gtgaaaattg ggtaatcccc agcaaccgct gcgtaatgtc
    15121 gtctatcttg tcgcgatcct ggcatcccta cattatttgt ggtctgtgaa gattatctca
    15181 ttgcagcccc tcatcttcgc agggctggct ttacagcttt tcaccttacg ttataagaag
    15241 ttccgtcgat gatggcgcta atttcgtgaa ttgtgcggct tgttgcaaat tacacggtgt
    15301 tgaaggttat ttacatgtta gctgttgatt atcttccctg ataagaccag tatttagctg
    15361 ccaattgcta cgaaatcgtt ataatgtgcg acctcgtcct ccctgacgca gtttttgcgc
    15421 tgcggaaaag gtgacattgg cgcaacgaag gtatattttg ttttttgccg gaggatagca
    15481 gcagatcgct gcacaatgtc cgtcaagtct aacattgata ctctggggca aaatagaccg
    15541 gcgtcccggc ctgctggaat ttatcgctat gcatacagct gtcggggcat acgctttaca
    15601 gacggcggtg aaacgcctgt cacaatcaca ctaaacaaag agtacggaac ccactcatgg
    15661 atattcgtaa gattaaaaaa ctgatcgagc tggttgaaga atcaggcatc tccgaactgg
    15721 aaatttctga aggcgaagag tcagtacgca ttagccgtgc agctcctgcc gcaagtttcc
    15781 ctgtgatgca acaagcttac gctgcaccaa tgatgcagca gccagctcaa tctaacgcag
    15841 ccgctccggc gaccgttcct tccatggaag cgccagcagc agcggaaatc agtggtcaca
    15901 tcgtacgctc cccgatggtt ggtactttct accgcacccc aagcccggac gcaaaagcgt
    15961 tcatcgaagt gggtcagaaa gtcaacgtgg gcgataccct gtgcatcgtt gaagccatga
    16021 aaatgatgaa ccagatcgaa gcggacaaat ccggtaccgt gaaagcaatt ctggtcgaaa
    16081 gtggacaacc ggtagaattt gacgagccgc tggtcgtcat cgagtaacga ggcgaacatg
    16141 ctggataaaa ttgttattgc caaccgcggc gagattgcat tgcgtattct tcgtgcctgt
    16201 aaagaactgg gcatcaagac tgtcgctgtg cactccagcg cggatcgcga tctaaaacac
    16261 gtattactgg cagatgaaac ggtctgtatt ggccctgctc cgtcagtaaa aagttatctg
    16321 aacatcccgg caatcatcag cgccgctgaa atcaccggcg cagtagcaat ccatccgggt
    16381 tacggcttcc tctccgagaa cgccaacttt gccgagcagg ttgaacgctc cggctttatc
    16441 ttcattggcc cgaaagcaga aaccattcgc ctgatgggcg acaaagtatc cgcaatcgcg
    16501 gcgatgaaaa aagcgggcgt cccttgcgta ccgggttctg acggcccgct gggcgacgat
    16561 atggataaaa accgtgccat tgctaaacgc attggttatc cggtgattat caaagcctcc
    16621 ggcggcggtg gtggtcgcgg tatgcgcgta gtgcgcggcg acgctgaact ggcacaatcc
    16681 atctccatga cccgtgcgga agcgaaagct gctttcagca acgatatggt ttacatggag
    16741 aaatacctgg aaaatcctcg ccacgtcgag attcaggtac tggctgacgg tcagggcaac
    16801 gctatctatc tggcggaacg tgactgctcc atgcagcgcc gccaccagaa agtggtcgaa
    16861 gaagcaccag caccgggcat taccccggaa ctacgtcgct acatcggcga acgttgcgct
    16921 aaagcgtgtg ttgatatcgg ctatcgcggt gcaggtactt tcgagttcct gttcgaaaac
    16981 ggcgagttct atttcatcga aatgaacacc cgtattcagg tagaacaccc ggttacagaa
    17041 atgatcaccg gcgttgacct gatcaaagaa cagctgcgta tcgctgccgg tcaaccgctg
    17101 tcgatcaagc aagaagaagt tcacgttcgc ggccatgcgg tggaatgtcg tatcaacgcc
    17161 gaagatccga acaccttcct gccaagccca ggcaaaatca cccgtttcca cgcacctggc
    17221 ggttttggag tgcgttggga gtctcatatc tacgcgggct acaccgtacc gccgtactat
    17281 gactcaatga tcggtaagct gatttgctac ggtgaaaacc gtgacgtggc gattgctcgc
    17341 atgaagaatg cgctgcagga gctgatcatc gacggtatca aaaccaacgt tgatctgcag
    17401 atccgcatca tgaatgacga gaacttccag catggtggca ctaacatcca ctatctggag
    17461 aaaaaactcg gtcttcagga aaaataattt gtgacgcgcg gtgttttcat cgcgcttagc
    17521 aaagaacgta atcaacgccg ggcctgttaa cgctgcccgg cgtttttatt gccgttagct
    17581 aacggtagcc tgcaatttcc gctttcaacg cgctcatttc agcatcgcca tcaacaattc
    17641 ccagctcaaa gcagtatttc tttgtttccc ctggctcaat aaagggcaga cgcccctgcg
    17701 ctcgttcttt atcacgaccg tcaggaaaac tattactgac ctccagtccc ataacatact
    17761 cacccgcgcc taaatttttc cactggataa agttgggtag ctgcatggtg tcgaatttct
    17821 cgtaaattcc ccaccccagt tcggcgttca ccagagcaac tttactcatc ccctgactgt
    17881 cgctattcaa cgtgagatag taaacctgct cgtcaaaacc gggctgtggg gtacaaatct
    17941 ccagatgact ggttaaccct tccgccgcat gcggagttcg tggcgttacg cttttagccg
    18001 acagcaagat ttctggcctg tggcgataat aaaggccagc cgtaattaaa gtgataaaga
    18061 atttgcagcg gctggcgggt gaaaccatga ttagtcacct tgtcttcaat gcgcagcaca
    18121 ttttcgccat aacggcagcg aatggtcctt tccagcgtca gattttcgcc gaacagtcgc
    18181 gtttcccgta ctttgccatt gatgacaaat tcaatgccgt cgtcggtccg ttctgtgcga
    18241 taccccacct cttctgctgg agtcgcagcc aaacgcccgt gtaaccccag cgtttctcct
    18301 tcatcctcac aaggcgtgcc aatgtagcta agtccgcagg tagtcagtaa ccccgcgtaa
    18361 aagctacgta aaaatccgtt gccctgagac tcaaaaaaag cgggggcgac aatgccgttg
    18421 ggggtgataa aagaaagcgc cgtgtccttg tagctggccc atgcaatatc cagcgcgcga
    18481 tcttttacca ccgtaaatgc cagtcccgca ccagtacgga catcaaacgc ttctgtaccc
    18541 tttgctctgc cactacgtaa ctcatagtgt ttaattccgg caacctgcga gagatcgcca
    18601 atccacgacc aaatttcgtt tttcattgtg ctgcttcccg aatggacatg tcatggcgct
    18661 ggatgaacgc ttcgatttcc gcaaggcgag acgttccctc cattgggcca cgtctggtta
    18721 ccgccagcgc accgcaggca ttggcatatt gcagcgctcg atggacgtca aaccctagct
    18781 ggcggcaggc aatccacgcg ccaccaaagc agtctcccgc accggtcggg tcaacttctt
    18841 ccaccggata gctctcaaca tgaaactgct cattcgcgga gtagtaactg gcaccttgat
    18901 tgccgcgctt cacaatcacc tctttaacac cttcttcaag aaaacctgcg attgcacgct
    18961 ccggcgtcga atgtggcgac aagagcaaca cttcgccctc gctgggcata tagatatccg
    19021 ttaactcaag aacaaagtgg agggcgtcac gcatttctgg aatatcgagc atctctttac
    19081 gaatattagg gtcgaaagaa ataacaccac catttgcttt aacgatagtt accgcttttt
    19141 ttactgcgtc taccatatga aacgaaaaca gggaagagcc cataatatga aaatgggtac
    19201 agtccttcag aatattttca tcaacatgct gcgccgataa tttaccgcaa gcggcattct
    19261 taatattaaa aataaaatcg cgatcgccag agttatgata agtaacaaag gcactcccgg
    19321 tagcttcctg aggtaagact gaaatccccc tgatatccac accgtcagca gccagacgat
    19381 gtatattaat atcaccaaag ccatcattcc ccacacagct aatgatgccg cacggaacgc
    19441 caagacgtgt cacctggtcg ataaaaatag cgggcgcacc actgggataa gggccgttcc
    19501 agatacctgg ttgatcaaat ggctggccga ttttactcgc cataatttca accagaattt
    19561 ctccaattgt gaagacctta ctcatatgat taattccttg aaatgattag ctgcgttttc
    19621 ttttatttct tacgtcaaaa taaacggcta caagaatgac taatccttta accaccatct
    19681 ggtaaaaata gggaacattc attaagttca tgccattatt aataacgccg atagttaatg
    19741 cgccgatcac cgtccccacc acggttccat agccgccgtt caggctggtg cctcccagta
    19801 cagccgcagc aatagcatcc agttcgtagc tcaacgcgga attcggttac ccggaataga
    19861 ggcgtgaggc gaggatcatc ccgctgatac ctgccagcac ggctgtaatc ataaagactt
    19921 taattttcaa gcgggtgaca tttatccctg aataaattgc cgcttcttta ttaccaccag
    19981 tgatataaat ttttcgtcca aaggttgttt tagtaagaat gaagtggtta atcagcaata
    20041 agatgattaa tatccagatt ggaataggta tatagagaaa ttctccattc cccagtgcca
    20101 gaaaggcatc atctttaatc atgatgggca taccatcggt cactatataa gcgataccac
    20161 ggaagatccc catagtagca acggtgacaa taaacgacgg gatccccgct atagccgtca
    20221 agccaccatt tatcatcccc agagccagtg ctgcaataat ggataatggg accaataaaa
    20281 taaatggaat tccccatgaa gccccaagcg cagcaaatgt gccaatcaat gcaatggttg
    20341 aaccgaccga taaatcaata tcgccaagca aaagtacata agtcattcca taggcggtaa
    20401 ttgcaattat cgaaacctga agaataatat tagtaatgtt gttggcggta aaaaaactgt
    20461 cattaataaa actgaacaga atgattaaca ccaccattcc actgaggata ccgccatatt
    20521 gcctgaagac ttttttaaaa ttatttgcaa tgggaatttc cgaaggtttc ggactggcaa
    20581 gagttttctc tgtcatttaa cgtactcctg ttgcagcggt cattacctgt gtctgcgtta
    20641 aattttcaca tgaataaata tcggcaatcg ttttattacg cattaccacc agacgatcgc
    20701 taagtgccat gacttctggc agttcagagg acaccagaat aattgctgtc cctgccgcag
    20761 ccagttgacg tatcacgtta tagatttcaa attttgcgcc gacatccact ccgcgcgttg
    20821 gctcatcgag caataaaata cgcggagttt tctccagcca cttcgataac acgattttct
    20881 gttgattacc gccgctcagc gctcccactg cctgatgtac agaactcact ttagtgttca
    20941 ttctggcaat tgattgcagc gtcttcgcct cttcttgtcg gtgattgatg agtcccatcc
    21001 gcgaagcgtt atattgaaac gccacatggt gcgtgttcat attgacgtcg tgcatcagca
    21061 ctaacccttc ttccttgcgg ttttcggtca caaaagcaat gccatgatca atagcctgtt
    21121 taggattagt gatcctgact tccttaccat caatcaacac agtcccctga tacgaaacga
    21181 ggccaaagag cgccttcatc acatcggaac gcccggcccc caccaggcca aaaaatccga
    21241 ggacctcccc cggacgcaca gcgaaactga tctctttaaa aagcgaagga tgggaaagat
    21301 tttttacttc cagttttgca ggaacatttt catccggtat ttccccaaga cgtgctggcc
    21361 aaatattttt catttcccga ccaaccattt gggcaatcaa atgctggata tcactgtttt
    21421 tcttttcatg cgtggcgata tactcaccgt cgcggatcac cgaaatatca tctgaaatcc
    21481 gcataatttc atccatgcga tgagagatat aaataaccgc acagcctcgc tgctttaatt
    21541 tttcaataat gttaaacaga atctccgctt cattatcact tagcgaagat gtcggttcat
    21601 ccataatcac gacatccgct gcatagctta aggctttagc aatttctacc agttgcctat
    21661 gtgccatcga catattgccg accatttcta gtgggtctag tggtatgccc cgttccgcga
    21721 ggagattctg gcaatctgcg agcatttttt tatcgttaat aatgccaaaa cgtcgcggtt
    21781 cattattggc ataaatattt tccgccacgg tcatattatt actcaggctc aattcctgaa
    21841 aaataatcgc aattcctaac tggcgtgaat gctgcggatt attaatggtg atagtttcac
    21901 cacgtagtgt tatcgttccc tgggttggcg gataaacacc ggccagtatt ttcattaatg
    21961 tcgatttacc ggcaccattc tcgcctaata aagtatgcac ccgcccttta cgtaaagaga
    22021 gggtaatatc ttttaatgct tgaacatgac caaatgtctt cgcaatatgc gaaacatcaa
    22081 gaataacatc cgtcataatg acctcggtgt cacaacaaaa cgccaataac gttttgttgt
    22141 gaggtgaaga tgtttactat ttacctgtat aaacgccggg ttgtaccggg atcatttttt
    22201 ctaccggttt accattgagg tgatcaacag cggcatcaat ggctttagca cccatttgat
    22261 cgggatattg ccgaatcacg gccgcaaacg ttttttcact gtcaacggca tcacgcgctt
    22321 ctttcatgcc atcaaaacca atgattttga tgttgtcgtt atgcgcagat tttgctgcga
    22381 taaccgctgc cagcgccgca tcatcaccaa agccaaacag accattcagg ttggcatttg
    22441 cctgcataat gttttgcgca gtcgttaacg cttcagcccg cgtaatacta ggctgttcag
    22501 caacaatttt aatatccggt gtatcgctta accctttctt aaaaccatca acgcgcgcaa
    22561 ccaccgattg tagtgccgga taagaaataa tggctacagt acctttacca ttcaaaagtt
    22621 tgcccattaa cttgcctgct tcaacgcctc ccgcatagtt atcggtcgca acatgcgata
    22681 cgaccggaac gccttccgca gcgacgtcga cggtgataac gggaataccg gccttatcgg
    22741 ctttaattat cgccgcctgg acgcctttcg aatcgaccgg agagataata atggcatcca
    22801 cttttttagt aataaaatcc tcaacatcag aaagttgttt gtttaaatcc tgattggcaa
    22861 atag
//
LOCUS       DAJVUJ010000055        22779 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.055, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000055 DAJVUJ010000000
VERSION     DAJVUJ010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22779
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.055"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(<1..456)
                     /locus_tag="OQQ48_001935"
     CDS             complement(<1..456)
                     /locus_tag="OQQ48_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1952725.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSLDASKIVPL
                     TVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFAQTTKGVLETG"
     gene            complement(486..1202)
                     /locus_tag="OQQ48_001936"
                     /pseudo
     CDS             complement(486..1202)
                     /locus_tag="OQQ48_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            1498..2274
                     /gene="exuR"
                     /locus_tag="OQQ48_001937"
     CDS             1498..2274
                     /gene="exuR"
                     /locus_tag="OQQ48_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HCS1952726.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            2619..3281
                     /locus_tag="OQQ48_001938"
     CDS             2619..3281
                     /locus_tag="OQQ48_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCS1952727.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            3285..3668
                     /gene="mzrA"
                     /locus_tag="OQQ48_001939"
     CDS             3285..3668
                     /gene="mzrA"
                     /locus_tag="OQQ48_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HCS1952728.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            3815..4183
                     /locus_tag="OQQ48_001940"
     CDS             3815..4183
                     /locus_tag="OQQ48_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
                     /protein_id="HCS1952729.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            4221..4526
                     /locus_tag="OQQ48_001941"
     CDS             4221..4526
                     /locus_tag="OQQ48_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCS1952730.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            4529..4933
                     /locus_tag="OQQ48_001942"
     CDS             4529..4933
                     /locus_tag="OQQ48_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCS1952731.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            4923..5222
                     /locus_tag="OQQ48_001943"
     CDS             4923..5222
                     /locus_tag="OQQ48_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HCS1952732.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            5408..5800
                     /locus_tag="OQQ48_001944"
     CDS             5408..5800
                     /locus_tag="OQQ48_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCS1952733.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSKFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            5870..6773
                     /locus_tag="OQQ48_001945"
                     /pseudo
     CDS             5870..6773
                     /locus_tag="OQQ48_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
     gene            7066..7431
                     /locus_tag="OQQ48_001946"
     CDS             7066..7431
                     /locus_tag="OQQ48_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCS1952734.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
     gene            7673..8029
                     /locus_tag="OQQ48_001947"
     CDS             7673..8029
                     /locus_tag="OQQ48_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCS1952735.1"
                     /translation="MQWYLAVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLEFPFLSLIYLAATIIPVIALCVRRLHDTDRSGVWALLYLVPIIGWLVLFVFACLEG
                     NSGSNRYGNDPKFGSN"
     gene            complement(8080..8976)
                     /gene="yhaJ"
                     /locus_tag="OQQ48_001948"
     CDS             complement(8080..8976)
                     /gene="yhaJ"
                     /locus_tag="OQQ48_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HCS1952736.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFSGK"
     gene            9081..9782
                     /locus_tag="OQQ48_001949"
     CDS             9081..9782
                     /locus_tag="OQQ48_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HCS1952737.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            9805..9969
                     /locus_tag="OQQ48_001950"
     CDS             9805..9969
                     /locus_tag="OQQ48_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1952738.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(10103..11413)
                     /locus_tag="OQQ48_001951"
     CDS             complement(10103..11413)
                     /locus_tag="OQQ48_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HCS1952739.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNS
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(11441..12773)
                     /locus_tag="OQQ48_001952"
                     /pseudo
     CDS             complement(11441..12773)
                     /locus_tag="OQQ48_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
     gene            complement(13047..14411)
                     /gene="tdcG"
                     /locus_tag="OQQ48_001953"
     CDS             complement(13047..14411)
                     /gene="tdcG"
                     /locus_tag="OQQ48_001953"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCS1952740.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGALSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(14483..14872)
                     /locus_tag="OQQ48_001954"
     CDS             complement(14483..14872)
                     /locus_tag="OQQ48_001954"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HCS1952741.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(14886..17180)
                     /gene="tdcE"
                     /locus_tag="OQQ48_001955"
     CDS             complement(14886..17180)
                     /gene="tdcE"
                     /locus_tag="OQQ48_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HCS1952742.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFEAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNIMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(17214..18422)
                     /gene="tdcD"
                     /locus_tag="OQQ48_001956"
     CDS             complement(17214..18422)
                     /gene="tdcD"
                     /locus_tag="OQQ48_001956"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HCS1952743.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSCGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(18448..19779)
                     /gene="tdcC"
                     /locus_tag="OQQ48_001957"
     CDS             complement(18448..19779)
                     /gene="tdcC"
                     /locus_tag="OQQ48_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HCS1952744.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(19801..20790)
                     /gene="tdcB"
                     /locus_tag="OQQ48_001958"
     CDS             complement(19801..20790)
                     /gene="tdcB"
                     /locus_tag="OQQ48_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HCS1952745.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(20889..21827)
                     /gene="tdcA"
                     /locus_tag="OQQ48_001959"
     CDS             complement(20889..21827)
                     /gene="tdcA"
                     /locus_tag="OQQ48_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HCS1952746.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRLLIEVG"
     gene            22016..22360
                     /gene="tdcR"
                     /locus_tag="OQQ48_001960"
     CDS             22016..22360
                     /gene="tdcR"
                     /locus_tag="OQQ48_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HCS1952747.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
BASE COUNT         5970 a         5764 c         5311 g         5734 t
ORIGIN      
        1 gcctgtctcc agaacacctt tggtggtttg agcaaacatc aggtttttag ccagagaacc
       61 gttagttaac gcatcggaag caaagatttt ggttgcggta aaagtggttg ccgcatcagc
      121 tttaatttct tcaccgcccc aggttactgt cagcggaaca atcttgctag catccaggct
      181 ttgaaccagt tgcgccgggg aaactaactg catttttaca tctttggtaa cgtcgttaga
      241 ccagattttg gtcatcaact ggtaagattg cagaccctgc cccggcagat attgcatttc
      301 tactgctttc ggcaacgcgg tgttatcggt ctgtgtcata tccagagcgg catcaacgtt
      361 ggccgttaca gtaatatcct tttgtactgc caatgcggag cctgcaacag aaaacatcgc
      421 tgcgaccaga agagattttg caaatacctt tttcattact attccttcgt ttattacaat
      481 gataattaat ggaccgccag ttcaaactcg ccattatctt tggtgatcca gttattatat
      541 ttgacaacga ttttgtcgta gccagatatg ctttcaagat ttctttcctg accagggaag
      601 atgtttttat ttacggtctg tatcttacaa ttttctttgc tattattttt acaataagcg
      661 atcgttaaaa tctttaagcg ttgattccct gcattaacta atttttgggc agcatttact
      721 tctaacttag caataggttg ctgaggtgga acactaacaa caatccccca aattaagcta
      781 acagttaact gcatagagaa atctttttta tctgtggcaa cgtcatcaat actgggaacc
      841 gcttcaaatt taacgcgtta atttttttct ttttgctctg gttccattgc aacaaagcgc
      901 acagttttgc tactaccggc tggtaaagcg aatttaggtg gcataacaac cagatgattt
      961 gcatcaccgg atttaatctc aacttcattt tcctgaggcg tcgatggatt atcgatacgg
     1021 aatactgtcg ccttgatgta ctgaacttca ttacttttag atattacgcg aacgttgcct
     1081 tcatcctgac tattaatgga aacagccata ggataaacag tcatatttga ctgtgccaaa
     1141 aaaggagcga acacgaataa aagcggcgca agccgtgata agagattaat tttcgccacc
     1201 atataaattt gatcttacaa tattgtttcc aaaaagttgg tgtattagag atgtagattt
     1261 attgtaagtc aatataaaaa agcattttta gttaatatta aatttaaagg ctgggaatta
     1321 acttgctgaa atttagtagc tttcacaatg acgatactta ttttttagga atttataaat
     1381 attcacaata ttaattcaat acattaattc aatcaccaaa tttatatatg accaatggca
     1441 tacccatgaa taaagtggta tgataacttc gtccgcattc cactccggga gacgcatatg
     1501 gaaatcactg aaccacgccg tttgtatcaa caacttgccg ctgacctgaa agagcgcatt
     1561 gaacagggcg tctatctggt gggtgataaa ctgcctgccg aacgctttat tgccgatgaa
     1621 aagaacgtca gccgaacggt agttcgtgaa gccatcatca tgctggaagt tgaaggctat
     1681 gtggaggtgc gtaaaggttc aggtattcat gtggtttcca accagccgcg ccatcagcag
     1741 gcggctgaca ataatatgga attcgccaat tacggtccgt ttgagttgct tcaggctcgc
     1801 cagctcatcg aaagtaatat tgccgagttc gcggcaactc aggtaacgaa acaagacatc
     1861 atgaaactga tggccattca ggaacaggcg cgcggcgaac aatgcttccg tgattccgag
     1921 tgggatttac agttccacat tcaggtagcc ctggcgacgc agaactccgc cctggcggcc
     1981 atcgttgaaa aaatgtggac ccagcgtagc cataacccgt actggaaaaa actacacgaa
     2041 cacattgatt cccgtaccgt tgataactgg tgtgatgacc acgatcaaat tctcaaggcg
     2101 ttaattcgca aagatcccca tgctgctaag ctggcaatgt ggcagcatct ggaaaacacc
     2161 aaaatcatgt tatttaacga aaccagcgac gatttcgagt tcaatgccga ccgctatcta
     2221 ttcgccgaaa acccggtagt gcatctcgat acggccacca gtggcagtaa atgaaactcc
     2281 tgtccgacag gcgctgcgac gcgcctgttc gcttcgtcgg taagcgaaaa gtataaagtg
     2341 tcagcctgtg taaatcctct cgccaccctc ccctgcattc agcaaaatca gactccacgg
     2401 acatgtaatt ttgataacga acaacgttga cctttgttac aattagattc aatttgaatt
     2461 tatgtttttg aatgctttct tatctcacga tttaacaggg aatagttcag gctgtgttga
     2521 tgtatcaaac ccgcagaaca taccaaaaca gcaataacat tgcggtagtg catcttttaa
     2581 aaccagcgtg gcgttaaccg attcaccagg aataatgaat ggaacttttg acccaattgc
     2641 tgcaagccct gtgggcgcag gattttgaaa ccctggccaa tccatcgatg attggcatgt
     2701 tgtattttgt cttgtttgta attttgttcc ttgaaaacgg cttgcttcca gcggcctttt
     2761 taccgggcga cagtttactg gtattggtcg gcgtgttgat tgcgaaaggc gcgatgggtt
     2821 acccgcaaac gattctgctg ctgaccgttg ccgccagcct cggctgctgg gtcagctata
     2881 ttcaggggcg atggctgggc aatacccgca ccgtacaaaa ctggctatct catttacccg
     2941 cgcattatca tcaacgcgca caccatcttt ttcataaaca cggtttatcg gcgctgttaa
     3001 ttggtcgctt tattgcgttt gtcagaacac tgctgccgac gattgccggg ttatcagggc
     3061 tgaataacgc gcgctttcag tttttcaact ggatgagcgg tctgctgtgg gtattgatcc
     3121 tgacaactct gggttacatg ctcggcaaaa cgccggtatt tttaaagtac gaggaccagc
     3181 tgatgtcatg cctgatgctg ctcccggtgg tgctgctggt gtttggcctg gcaggttctc
     3241 tggtcgtgtt atggaaaaag aaatatggaa atcgggggta agggatgcaa atacctcgca
     3301 tgtcgcttcg ccagctagcc tggtccggca ctgttttact tctggtcggc acgctgttgc
     3361 tggcctggtc cgcggttcgc cagcaagagt ctacgctggc gattcgtgcc gttcatcaag
     3421 gcacaacgat gccagacggt ttttcgatct ggcatcacct tgacgctcat ggcattcctt
     3481 tcaaaagtat cacccccaaa aacgacactc tgttaattac atttgactcc agcgaccaga
     3541 gcgccgccgc aaaagcggtc ctcgacagaa cattgcccca tggctacatc attgcgcagc
     3601 aggacaataa cagtcaggct atgcagtggc tgacccggtt acgggataac tctcatcgct
     3661 tcggataact tcctgttatc cgaaacatat cactcacttt ggtgatttca ccgtaactgt
     3721 ctatgattaa tgaggcggtg gttaacacta ccgcttcgtt gtctggacaa tcgttccttt
     3781 gtaacaggtc cgaatcacaa tggaaggttc aagaatgaaa taccgcatcg ctttagctgt
     3841 ttctctcttt gctcttagtg ccggtagtta tgccactacc ctgtgtcagg aaaaggagca
     3901 aaatatcctt aaggagatca gctatgccga aaaacaccaa aaccagaatc gtattgacgg
     3961 tctgaataaa gccctgagtg aagtccgggc caactgttca gatagccagc tgcgtgccga
     4021 tcatcacaag aaaatcgcaa agcagaaaga tgaggtggcg gaacgccagc aagatttagc
     4081 cgaggcgaag caaaaaggcg atgccgataa gattgccaaa cgcgaacgga aactggcaga
     4141 agcgcaggaa gagctgaaaa agctggaagc gcgcgactac taactcacaa tagtcactac
     4201 ttactcacct ggagaaaact atgtcgaaag aacacactac ggaacatctg cgtgctgagt
     4261 tgaaatccct ttccgatacg ctggaagagg tgcttagctc atctggcgag aagtcgaaag
     4321 aagagttgag taagattcgt agcaaagcgg agcaggcact gaaacagagc cgttatcgcc
     4381 tgggtgaaac cggtgatgcc atcgccaaac aaacccgtgt cgcggcggcg cgtgctgatg
     4441 agtatgtgcg tgaaaatccg tggaccggcg tgggaattgg cgctgcaatc ggtgtagtgc
     4501 tcggcgttct gctgtcgcgt cgttaattat ggcggacact catcacgcgc aagggcccgg
     4561 taaaagcgtt ctgggcatcg ggcagcgaat tgtttccatc atggttgaaa tggtggaaac
     4621 acgtctgcgg ctggcggtgg tggagctgga agaggaaaaa gctaatctct ttcaactttt
     4681 actgatgctg ggcctgacga tgcttttcgc tgcatttggt cttatgagcc tgatggtgct
     4741 aattatttgg gcggttgatc cgcaatatcg cctgaatgcg atgattgcca ccaccgtggt
     4801 gttgctgcta ctggcactga ttggcggtat ctggacgtta cgtaaatcgc gtaaatccac
     4861 cttgctgcgc catacacgcc atgagctggc aaacgatcgg caactgctcg aggaggagtc
     4921 ccgtgagcag taaagtcgaa cgtgaacgac gtaaggcgca actgcttagc cagatccagc
     4981 aacaacggct ggatctttcc gccagtcgtc gtgaatggct ggaggcaaca ggcgcttacg
     5041 atcgtcgctg gaatatgctg ctaagtctgc gctcctgggc gctggttggc agtagcgtga
     5101 tggcgatctg gacgattcgc catcctaata tgctggtccg ctgggccaga cgcggttttg
     5161 gcgtatggag cgcctggcgt ctggttaaaa cgaccctcaa gcagcaacag cttcgcggtt
     5221 aacttccctc tggccggagc cattccggcc ctatccctca aattttttga agatttttga
     5281 cagttttcct tgctaacaat catcattcac cacgtttatg attctctcca tcgacagcaa
     5341 cgacgctaat accgcgccct tgcacaaaaa aacaatcagc agcctgagtg gcgcagtgga
     5401 gagtatgatg aaaaaattag aagatgttgg tgtactggta gcgcgcattt taatgccgat
     5461 tctgtttatt accgcaggct ggggaaaaat caccgcttac tctggtaccc aacaatatat
     5521 ggaagcaatg ggtgtcccgg gttttatgct gccgctggtg attctgctcg agttcggtgg
     5581 tggtctggca atcctgttcg gtttcctgac tcgcaccaca gccctgttta ctgcgggctt
     5641 tacgctgctg acggcatttt tatttcacag caagtttgct gaaggcgtca actcgctgat
     5701 gttcatgaaa aacctgacaa tttctggcgg attcctgctg ctggcaatta ccggtccggg
     5761 cgcgtatagc atcgaccgcc tgctgaataa aaagtggtaa gcacgcgcta tacttaacga
     5821 taaaaaacga ggaggaagct cctcgttttt gctattggag gagagaaaaa tggggcaact
     5881 gattgacggc gtctggcatg acacctggcc ccactggcac aggcggtttt atcgcagaga
     5941 aagatcgtta tcatctctat gtttcactcg cctgcccgtg ggcgcaccgc acgctgatca
     6001 tgcgcaaact caaaggactg gaaccgttta tttccgtttc cgtagtgaac ccgctgatgc
     6061 tggaaaacgg ctggaccttt gatgacagtt ttccgggagc aaccggagat acgctctatc
     6121 aacatgaatt tctgtatcag ctttatctcc acgccgatcc acactacagc ggacgagtta
     6181 ctgttcccgt gctgtgggac aaaaagaacc acaccattgt cagcaacgaa tcagcagaaa
     6241 tcatccgcat gttcaatacc gcgtttgatg cgctgggcgc aaaagcgggt gattactacc
     6301 caccagccct gcaaacgaaa attgacgaac ttaacggctg gatttatgac accgttaaca
     6361 acggcgtgta taaagctggt tttgccacca gccagcaagc ctacgacgag gcggtggcga
     6421 aagtgtttga atcgctggcg cgactggagc agattttagg ccagcatcgt tacctgaccg
     6481 gcaaccagct aaccgaagcc gatattcgcc tgtggactac gctggtgcgt tttgatccag
     6541 tgtatgtgac ccacttcaag tgtgataagc accgcatcag cgattacctg aatctgtatg
     6601 gcttcctgcg cgatatctac cagatgccgg ggatcgccga aactgtcaat ttcgatcaca
     6661 tccgtaatca ttacttccgc agccataaga ccatcaaccc tacggggatt atttcaattg
     6721 gtccgtggca ggatctcgat gaaccgcatg gacgagatgt tcgcttcggt taaaaaaagg
     6781 caccttacga ggtgcctctg ataattagct aagtgcatgt tgttcatgtc ggatgcggcg
     6841 tgaacgcctt atcaaaccta caaaacgttt atattcaata tgttaaaatg accgcgtagg
     6901 ccagataagc gtagcgcatc tggcgatttt gcgcttgtca tcagtcttag cgcaccttca
     6961 cggcacttta ttaattcctt gaacgaatat ttactgccat ttcattcacg tctattctta
     7021 atttgctgct ttaaaaacaa gtgattgagc aaaattgagg caaaaatgga ctggtatctg
     7081 aaagtactaa aaaattatgt cggtttccgg ggtcgtgcgc ggcgcaaaga gtactggatg
     7141 tttattctgg tcaacatcat ctttacgttc gtgctggggc tgctggataa aatgttaggc
     7201 tggcaacgcg ccggtggcga aggcatcctg acgacaatct acggtattct ggtgttttta
     7261 ccgtggtggg cggttcagtt ccgccgcctg cacgacaccg accgctcggc gtggtgggca
     7321 ctgttgttct taatcccgtt tatcggctgg cttattatca tcgtctttaa ctgccaggcg
     7381 ggtacgccag gcgaaaaccg ctttggacca gatccgaaac tggaacctta aaacaaatct
     7441 gctcgccgga tgtctgactc atccggcttt tatcatcatg ttgatgaatg aataatttac
     7501 taatgtttat ttaaaatatt tcaatgtcgt tatataaatt acctataaaa aacaaccatg
     7561 tatttatctt aaatatcaaa acctttataa catccgaaaa agaaagatac gaatagcatt
     7621 tacacaatac ccggaataga ctttcgtcac ttttaattaa agggatgttt ttatgcagtg
     7681 gtatttagcc gtattaaaaa attatgttgg tttctctggt cgcgcacgtc gtaaagagta
     7741 ctggatgttt actctgatta acgccatcgt cggcgctatt atcaatgtca ttcaattgat
     7801 tttaggttta gagtttccat tcctttctct catttacctg gcagcaacaa tcattccggt
     7861 cattgctctg tgtgttcgtc gtctgcacga tactgaccgt tcaggtgtgt gggcgcttct
     7921 ctatctggta ccgatcattg gctggttagt tctgtttgta tttgcctgtt tagaaggcaa
     7981 ttctggcagc aatcgttacg gaaacgatcc taagtttggt tcaaattaat cttagaattg
     8041 gggcgatatt tcgccccttt ttattaacaa taataaatat tattttccgc taaaaagttt
     8101 gggaatttcc cgcagacacc aggatttcgc ttcccccata ctgtcacgac gccaggccat
     8161 aataatatcg atctcgctgg tcgattccgg gctgacgaca cgcaaccgcc cttccgcaat
     8221 atctttttcg accatcggat acggcatcgt cgccacgcca agccccgcca gtaatgcctg
     8281 acgtttatct tcaatcgtgc tcaccgttaa gcgcggctgt ttgtccagca gctgtacggt
     8341 caacaccggg cgctcacgag cggtatccgc taccgcaatt ccacgatatt tcacgcgcgt
     8401 cacttcagat aacggttccg gctcctgatg aatcgggtga tcaggcgcgg caacgtagac
     8461 gttcattaag gtatagagct tgcgcgagtt gatctccgac gaggaacgaa aatgcatatc
     8521 cggcgcgata acgatatccg cccgcccctg ctccagtcgt tcccacgccc ccgccagcac
     8581 ttcggtgatg attgccagtt gggtattggc ttttgccgcc agtttgtcga ttaacgggaa
     8641 aaaggcaggt gtcggtacca gcgcctcggt cacaatggtg agatgcgttt cccaaccgcg
     8701 cgcgagggct tccgcatcgg tagtcagttt atctgctgct tccagcaaaa cgcgccctcg
     8761 ctccagcaac atcctcccga cattggtgaa tttggtgcga tggcccgagc ggtcaaacag
     8821 caccacatcc agctcttctt ccagtttttg catggtgtag ctaagtgcgg aaggcacgcg
     8881 tcccagctca tccgccgccg ccgcaaaact gccccggcga tcgatcgcat ccataacccg
     8941 tagtgcttcc agcgttaatg ccctttcttt ggccatttcg ttctcattca ggaaatttga
     9001 acataccggg cagaatatct ggctaacaat gtagcgtcca gccccttacc ataaaaggaa
     9061 gtaaagagag gtcaagaatt atgattacta cccgaactgc caggcagtgt ggacaagcag
     9121 actacggatg gttgcaggcc cggtatactt tttcctttgg acactacttc gacccgaaat
     9181 tgttaggcta tgcctccctg cgtgtgctta accaggaagt gctggcccca ggtgccgcct
     9241 ttcagccgcg aacctatccc aaagtcgata ttttaaatgt gattctggat ggagaagcag
     9301 agtatcgcga cagcgaaggc aatcatgttc aggccagcgc cggtgaggcg ttgctgctct
     9361 ctacccagcc gggtgtcagc tacagcgaac acaatctcag caaagacaaa ccgttaacac
     9421 gaatgcagct ttggctggac gcctgcccgc agcgagagaa tccgctgatt caaaaactgg
     9481 cgcttaatat gggcaagcag caattaatcg cctcgccaga gggtacgatg ggaagcctgc
     9541 aattacgcca gcaagtgtgg ctgcaccata tcgtgctcga caaaggcgaa agtgcgaatt
     9601 tccagttgca tgggccacgc gcgtatttgc aatcgattca cgggaaattt catgcgctta
     9661 cgcatcatga agagaaagcg gcgctgacct gcggtgatgg ggcgtttatt cgtgacgagg
     9721 ctaacattac gctggttgcc gattccccac tgcgcgcttt gctgatagat ttgcctgtct
     9781 agttgttttt acagggagat gatgatgagt aaaaaatcgg ccaaaaagcg ccagccagtg
     9841 aagcccgtgg tggcgaaaga acctgctcgc accgccaaaa attttggcta tgaagagatg
     9901 ttgagcgagc tggaagccat cgtcgcggat gctgaaacgc gtttagccga ggatgaagct
     9961 accgcgtaaa gacaggttgc cggatgcggc gaaaacgcct tatccggcct acaaaatcca
    10021 acaacattaa cgaattaaac aacccgtagg ccggataagg tgcgccagca tcgcatccgg
    10081 cacaatactc aaaacctggc gtttatctgg ccttgctcgc cataatctcg ataatctgcc
    10141 gatccgtttg ctgcatcgaa tggcttgcta acgcacacag gttggcaatc gactgctcaa
    10201 catcatgcgc cacgatccct tcattgccgg tcacggcggt atcatccagc gccattaaca
    10261 ccgctttcca cgcagccgaa gcactggtcg aaaccttcat cgcgcagctg ttcgacgcac
    10321 catcgcaaat catgccgctg acatcgccga tcatactgct gatcgccatc gagatagttt
    10381 cataacgccc atccaccagc catgccatcc cggcggcggc ccccattgct gcggtcgttg
    10441 cggcacaaag tgcagacaaa cgcggtaact ggttatggat gtaaattgcg ctcaaatgag
    10501 aaagcatcag cgcacgcgcc agtcgttcat catccgctcc gaagtgttct gctacaacca
    10561 ccacaggcat tgttgcggtg atcccctggt tacccgagcc ggagttactc atagccggaa
    10621 gcgtagcgcc gcccatacgc gcatcggatg ccgcgctggt acgaatcaca atggatgaag
    10681 agagatcttt cgccagcaaa ccgcgctcgc actgtttttc cagcgtcgcg ccaatatgca
    10741 gcccccactt accgctcaaa ccttcctgcg ataacgcaca atttagcttc gcggaatcga
    10801 gaataaagcg gatcgccgca aacgggactt cattgacgaa cttcaggatc tcagccagcg
    10861 tcgttctgga aagcaccgtc agcggagact cttgctcgcc ctctgccaca cacgcctgct
    10921 gggtaaacac cacactattg tgcgtctcaa tatgcacaat gttggtatgc ccgccgacga
    10981 tggtgacaca cgcccacttc tcaccgttcc agactttggc gcgtgagaag aggatttcat
    11041 tgcaaggttc ctggatctta acggagactt tccccgccgc cagcagtgct ttggcatcgg
    11101 caattgcctg cgcagttgcg tctttcagca cttccagccc ggcgttggca tttccaccta
    11161 acgcccccag cgccgccgca atcggcagcc ccaccattcc cgtgccggga acggtgacgc
    11221 ccagaccgtt cttcatcaga tttggcgaaa cccaggcttc tacacgttca accggacctt
    11281 ccagttctgc cgcagcaacc gccgccgcca gcgccagtga aatcggttca gtacatccca
    11341 gcgccggttt tacttcctcc tgaacggcga ggatgtaacg ctgccataac ggatttaaag
    11401 tcgaatcaaa catattaaaa accttaaaat ttcaggtaaa tcaagaaaat gccaggaacg
    11461 gagaaacaca aagcaacaaa ccagtgacga taatcaggta cagagacatc cctttgtatt
    11521 tgtgcaatgc cggtactttg taaaccagcc acgccgggat caggcaccct accatgccga
    11581 aaatcgggct acagatagag gtgaagctca acaccggtgc gttcagtacg atggcgctcc
    11641 aggccagcaa aatggcgaaa atcatgatgc cgcgctgaac aagattttcg ttaatcttct
    11701 cggcaggcat cttgcgacgc aggatgttca ttacgatccc ttgcgttgct tcgcgaaagc
    11761 ctaaatagac gccaaagaat gcagtcatga cagcaaagat attgagaatg acgctgacca
    11821 ctttcaccca cgctgcgccg tcaccgctaa taaactgtgc ggcaatcgcc agcgcggaaa
    11881 tattctgctc gtaggctttt accgcttcgt catgtcccat cgccagcgtg aacgacacgg
    11941 cgtagaaaaa gacggtgaca aataaaatgc caaacgcgat attcattgcc cgcaatgctt
    12001 tatgccgcgc cacttcaatt gatttttccc gcgagcgata agagatcacc atcgggctta
    12061 acgtctggat aaacagaatc gacgtcaggg taaacggcag cgtaataatg gcgtttttca
    12121 ccagcagccc cagcggcggt agcgaaccga cgttatacag atgccacatt cccaccatcg
    12181 acacgcccag cgcagcgact accagcagct tggtcagcac catgccggtc gaaattttga
    12241 ataacaattt ctcgccgcgt gaagagatcg ccaccagaat gcaaatcagt accagaccat
    12301 aaaagggact gtctgacagc accccttccg tcacgccgaa ggtatgcaga taggaagcac
    12361 tatcgttggt gatggcggtg gaatagacga acatccaaat caccagcatt acgaaataga
    12421 gcgcacctaa caggatgccc cagtttttac ctaaataacc gctaatgaca ctcgggtaat
    12481 ctttacattc tgtgtgattc tgccagcgta ttaataaaca accgctgaaa cagatacatt
    12541 gccgggtaac caatcaccga tgagagcaaa aatacccaca atcccatcaa accgacctgc
    12601 accgggagaa aaacaatccc cgcgccaatc gccatcccga tactcataat cacccagccg
    12661 gtgtcagtac tgtcgaattt gatcgcttct cgccactcgc tctcactcat tccagcacga
    12721 cccgccgggg tcgaagcgtc tgcaatgacg cctttattcg atgcaatttc cataatttct
    12781 cgctcaatat tttgtagggc ttattatttt tttccgagcc gcatcaaggc gatatgcggt
    12841 ctacgttttt gcaggcgagt tattagaata aagaaatgat acgcgcgagg tcagagaaaa
    12901 tcttcacaat caagactttg aaataacgga gatggataag aattttctac taaattaatc
    12961 gcaagagaat attcattctc tatttgcggc agatcacaaa aaaaggtgca catttgtgca
    13021 cccaaggatg aaagctgaca gcaatgtcag ccgcagacca ctttaatggc cagtcctccg
    13081 cgtgatgttt cgcggtattt atcgttcata tctttgccgg tttcatacat cgtctcgatc
    13141 actttatcga gtgaaacacg cggtgccgag gtgcggcgca tcgccatccg cgcggcgttt
    13201 actgctttca cggcattaat ggcattacgt tcaatgcacg ggatttgtac ctgtccggca
    13261 accggatcgc aggtcagccc aaggttatgc tccatcgcga tttccgccgc attgcatacc
    13321 tgcgccggac tgccgcccag tagttcagtt aaccctgccg ccgccattga acaggccacg
    13381 ccaatctccc cctgacagcc gacctccgcg ccagagatgg aggcgttcat tttatacagc
    13441 gcgccaatag cccccgctgc cagaaaatag cgggcaattg accgctcgtt taccggacga
    13501 cggaacttat cgtaataagc cagcactgcc ggaataatgc cgcacgcacc gttagtcggt
    13561 gccgttacca cgcgcccgcc agctgcgttt tcttcactaa ccgccagcgc gtacatgttg
    13621 atccagtcga tgacattcat cggatcgtta gagatgttat cgctagaaac cagctgacga
    13681 cgcagcgcta cggcacgacg cggcacattg agcggaccag gcagcacgcc ttcagtgttc
    13741 atcccgcgtt caataccgtc atgcatcact tgccagatac gggcaaaccc tgcgtcaatt
    13801 tccgctttgc tgcgcagcgc cagctcgttg tgcatcatca ggccagatat agacaggccg
    13861 ttgtaatcac acattttcag cagttcacct gctgagtgga aatcgtaagg tacggacgtt
    13921 tcgacatcgt gcgacaggcc gaagtgttct tcttcgacaa taaacccgcc gccgacagag
    13981 taataggttt tacttaatag cgcttcctgt cccttccagg cagtgatccg cattccgttc
    14041 tcatggcgag gcaacatttc gggatggaag ataatgttct ttgctacagg aaaatcaaca
    14101 atatgcgcac cagatgccac tggcagccgc ccgctgcgcg ttactaactc tataaatgca
    14161 gggatctcat caatgacaac atcctgcgga ctgtttcctg ccagtcccat gataatggcg
    14221 acatccgtgg catggccttt gcccgtcagt gacaacgccc catacagatc gaccacaata
    14281 tggctcgtcg cggttaataa gccgctactt tccagccgat caataaaact ttttccggca
    14341 ttcattggcc ccacggtatg cgaactggag ggaccaatcc caattttgaa aatatcgaat
    14401 gcactaatca tatccacacc ctcggattgc cgttcagtga agtggagcgg aacgaccttg
    14461 cgaccgtccc gctcacgagg ctttacgcac tacgtactgc gatagcttca atttccagct
    14521 tcacatcttt cggcaaacgc gcaacctgca cacagctccg ggtcgggtag gtcgcctgat
    14581 gctcatcgaa gaactgctta tagacttcat tgatggtggc aaaatcattc agatcggtga
    14641 tgaacacggt catcttgatg atatcgccca cgctcagccc ggcagcaacc acgatcgctt
    14701 tgacgttttc gaggcttaaa cgcgcctgat cttgcacatc agccgggatc tcaccggtct
    14761 gcgggcaaac cggaatttgc ccggaggtga agaccatgct gcctaaatca acgccctgaa
    14821 cataagggcc gattgcgcct ggggcacgtt gcgtttcgat aatctttttc atacatcctc
    14881 cggcgtcaga gcgcctgggt aaaagtacgt gaaataacat cctgttgctg ttcacgggtc
    14941 agtgcgttga agcgcacggc gtagccagag acacggattg tcaggttagg atatttttcc
    15001 gggtgctcga tggcatccag cagcatttcc cgattcatta tgttgacgtt gaggtgttga
    15061 ccgccttcga catccgcttc gtggtggaaa tacccatcca gcaggccgac aaggttggtt
    15121 ttacgtactg gatcttcttt gcccagcgcc gcaggaacga ttgagaaggt gtacgagatc
    15181 ccatctttgg cgtaggtgaa cggcagtttc gccaccgacg tcaatgaggc cacggcacct
    15241 ttgcggtcac gaccatgcat cgggttagcg cccggcgcga acggtgttcc ggcgcgacga
    15301 ccgtccggcg tattaccggt tttctggccg tacaccacgt tagaagtgat agtcagaatc
    15361 gactgggtag ggacggcgtt gcgataggtt ggcagcgctt taattttctt cataaagcgt
    15421 tcaaccaggt cgcaggcaat gctgtctacg cgctcgtcgt tgttgccgta ctgcggatat
    15481 tcaccgtcga tttcaaagtc caccgccagg ccgttttcgt cacggattgg tttcacgcgg
    15541 gcatatttga tggcagacag ggagtccgtc gccaccgaca ggcccgcgat gccgcatgcc
    15601 atagtgcgat agacatcacg atcgtgcagc gccatcagcg aagcttcgta gctgtacttg
    15661 tcgtgcatgt agtgaatgat attcagcgcg ctgatgtact gcaccgccag ccagtccatg
    15721 aagtgatcga ggctgtccat cactttgtcg taatccagca cgtcgtccat cagcggtgct
    15781 gttttcggcc cgacctgaat cttcagcttc tcgtccaccc cgccgttaat tgcgtagagc
    15841 agcgttttcg ccaggttagc gcgtgcacca aagaactgca tttgcttacc aatcaccatt
    15901 gggctgacgc agcaggcaat cgcgtaatcg tcgctgttga agtcagtacg catcagatcg
    15961 tcattttcat actgcaagga agaggtgacg atcgacacct gcgcggcata ttttttgaag
    16021 gcaatcggta attcttccga ccaaagaatg gtcaggttag gttccggtgc cggccccata
    16081 gtgtgcaggg tgtgcaaata gcggaaggag tttttggtca ccagcgtacg accgtccagc
    16141 cccatcccgc cgatcacttc cgtcgcccag attgggtcgc cggagaacag cgaatcaaat
    16201 tccggtgtac gcaggaagcg taccatacgg atcttcatga tgaagtgatc gatcagttcc
    16261 tgtgcctgct gctcattgag tacgccagct tcaaagtcgc gctcaatgta gatatcgagg
    16321 aacgatgccg tgcggcccag cgacatcgcg ccgccatttt gcgatttcac tgccgccaga
    16381 taagcgaagt agagccactg caccgcttct tgcgcattct gcgccgggcg agagatatca
    16441 aagccatatt tcgccgccat ttcctgaatc tgcaacagcg catgacgatg ctctgccagc
    16501 tcctcacgca gacggatggt ggcttccaga tcctcgcctt tttccagacg agactggaga
    16561 tcggcaaatt gcagttcgcg ttcacgtacc agataactga tgccatacag cgctacgcgg
    16621 cgatagtcac cgataatgcg cccacggcca tagccatctg gtaaaccggt cagcacgcca
    16681 gatttacggc agcgcagcat atccggtgag taaacatcaa atacgccctg gttatgggtt
    16741 ttacgcagat cggtaaacag atattcaaat tcactgtcca tttcacggcc ataggcgtgg
    16801 aatgaacttt taatcatatt aatgccaccg aacgggtgta gcgcacgttt caacggcgca
    16861 tccgtttgca ggccaacaat tttttccagc ggctggttaa tatatcccgc atcatgagcg
    16921 gtaattgtgg tggcaatatt ggtatcgaaa tcaaccggcg cgtgggttgc gttttcgata
    16981 cggatacctt ccattacttt ttcccacaat tccgtggtgg caggcgtcgc ttcggcgagg
    17041 aaagattcat cgccttcata cggtgtatag ttatgttgaa taaaatcgcg gacattaatt
    17101 tcgtttttcc agtccgtacc tttaaagcca agccatgcgt cggcgtacag cttatcgctg
    17161 gtatcaatat ctaccttcat gaaaaataat ctctctacaa tacttcaact aaattatgca
    17221 aattctgcgg gcgcgttaac tttgcctaaa tgaatggcat ccaaagcaat cattttttct
    17281 tcgttagtcg gaataacagc gcaaatgaca cgcgcatttt cactggaaac aattcgctca
    17341 ccacaggagt tagagcgatt attcatttct gtatcaatct ctacgcctaa tacagccaaa
    17401 tgttccatga ccagacgacg aattaagctt gaattctctc ctattccgcc ggtgaatata
    17461 attccatcca ggcgacgtaa tgaagctgcg tgtccggcaa tatgacgggc aattcggtga
    17521 acaaaggttt taattgccag ttgcgcgcgt tcgtgacctt catgccaggc tttttccaga
    17581 acacgtaaat ccgaagaaag accggaaata cctaataatc ccgactcttt attcactacg
    17641 cgttccaggt cacccaagct ctggttggtt tggctggcga cccaggacat cgcaccaaaa
    17701 tcgacatcgc cactgcgggt acccatcatc aagccttcca gcggcgtcat tcccattgag
    17761 gtgtcaacac tctgaccgtt gcgaactgcg cagattgacg cgccattgcc aagatgcgcc
    17821 acaaccaggc cggagtcatc ttccgctaga ttcagcaaag aatgtgcgcg ctgggaaaca
    17881 tagcggtgcg acgtgccgtg gaaaccataa cggcgtacac ctaactcttc ataatatttc
    17941 cacggcaggc cgtataaata agcttccgga gccatcgtct ggtggaaact ggtatcaaat
    18001 accgccacct gagttacgcc cggaaataat tgctgcgccg attcaatacc acttaaattg
    18061 gcgtaattat gcaggggtgc cagtggagaa acgcgacgga tattatcaat gacctcatcg
    18121 gtaataatgg cggactcggt aaaaatactg ccgccgtgag cgatgcggtg gccaattaag
    18181 gccacactgt catttaaatt ccgtttttcc agttcaaatg caattgcctt caatgcacct
    18241 tcgtagctgt ggtgagccag cggtgctggc tctcccccat ttacggataa gaatgcattt
    18301 tctgagttaa taccgtcggc aatacctgac attaatactt cacagtcgct ggcatcgagt
    18361 acggaaaact taatcgaaga cgaaccacag ttaataacca aaacaaccgg aaattcattc
    18421 atctcttttc tcatcctgag ttacggatta aaacagtttg tatacgatgt tcaggatggt
    18481 cagcagacca atcacggtaa caaacacgtt atccagacga ccacggtatt tcgccagaga
    18541 cggtgcttta cggatggcat acatcggcaa caggcacagc agggatgcga taatcggtgc
    18601 gcccatggct tcaataaggt cgaggatgtt cgggttggcg taggcaacaa cccaggtgga
    18661 gcccatgatg aagatcatgc tgatagtgtt cagtttaccc agcgacactt tagttttgtc
    18721 gcctttataa ccgaacttca gaatcagacc attcaagcct tccagcgtcc ccagatagtg
    18781 accgaagaaa gatttgaaga tagccacgag tgcgatgatg gaagccgcat attccagtgt
    18841 aatcgcgaac gttgttttgg taccggtcat ggacgcaaag tggttagcca gataagaaag
    18901 cactggaata ttctgcgctt tggcttccgc catgttggcc ggagacagcg taaacaggca
    18961 gctaaaggca aagaacatca ccactgcaac catcagcatg ctggcacgag aaatgatttg
    19021 ggaacattta cgttcggtga agtcgcgacc gaagtctttc tcatactctt cacgtttaga
    19081 aaccacgaag gaagagacga ttggcgagaa gttaaaggag aaaaccatga tggaaatccc
    19141 cagccagaca gtgatcagga taccgtcatg accggttaac gacagcgaac cgaggtcaac
    19201 ctggtcgata actgcagagt tccagtaagg gatcagcgac agagaaatca gcaccaggct
    19261 ggcgataaac ggccatacca ggtagctcat cactttaacc atcagatcct taccaaacca
    19321 gatgacgaaa gccatcagca gcaacaggaa cagcgccaca aagccgcgat tcagcggtgc
    19381 aaatccgagc tggttttccc agaacgtcat aaaggtattg gtaatagtaa cgccataaat
    19441 ccacagcagt gggcaaatcg cgaagaagta caggaacgtg ataaccacgc cgccagtttt
    19501 accaaaatgc tcttccaccg tttccgtaat gttgccggaa gggttagagc cagaaagaca
    19561 cagacgcgcc agcgcccggt ggcaataaaa tgcgatgggg tatgccaata ccaacatcag
    19621 aagaatcggg atcagtccgc caaaacctgc gcggataggg aagaacagca ccccggcgcc
    19681 gattgccgta ccaaacaagc ctaacgtcca tgtggtatct gatttacgcc aggacgattg
    19741 ttttgtctgg ctggatacaa tgctatctga agtactcata tcctatcctc aacgaattaa
    19801 ttaagcgtca acgaaaccgg tgatttgaga gacgcgagaa agatcgatat tgccgccgga
    19861 aataatactg acggtttttc tgttttgaat atattggtct aatttaccgc ttaataatgc
    19921 agcacatgcc agagcacctg cgccttcggt gacgacttta ttgcgctgaa ttaaggcaat
    19981 catactgttt ctgatttcgt cttcgctgac cagcacgatg tcatcgacta attcacgaac
    20041 gatttcgtaa gttaaattac ccgggcggga gacatcacaa ccatccgcca gggtgccggt
    20101 agttcggtgc gtggttattt ctccggagtg gaaagaagcc gccatgccgt gaacgttttc
    20161 tgactgtacg ccaataacac gaatggtcgg gttaatagat ttaattgcca ccgcaatacc
    20221 agcaattaaa ccaccaccac caattggcac aatcacgtta tcgacatcat agagatcttc
    20281 cataatttcc agaccaatcg ttccctggcc agcaatcact ttcggatcat cgtaaggtgg
    20341 gataaaaata cgaccttcca tttcgacaat ttcgctcact ttagcgatag tgtcgttgaa
    20401 gttatcacca tgcagaacga cttctgcgga gtagtcgcac gttgccgcta ctttggattt
    20461 tggcgcacct tttggcatca ccactttacc gtcgataccc agcatcgcgc aggagagcga
    20521 aaccccttgc gcatggttgc ccgcagaaca ggccaccacg cctttgcgtt tttccgcatc
    20581 ggtcagtgaa cttaatttat taaatgcgcc acgaatttta aatgaacccg tacgctgcat
    20641 attttcaaac ttcaggaata tttcaccttt gcaacgttca ctaaaatagt tggagcgagg
    20701 catgcctgtt ttataaattc gcccagccag tcgttgtttc gcttcaataa tatcatcaat
    20761 agcaaccggc agatcgtatg taatatgcat tataaaacct cttcgcctga attaaatatg
    20821 tagataaccg taaccgacac ctgcaaaaca gacaggtgga ttatttatgt ttaaataaaa
    20881 acaaataact aaccaacttc tattaatagc cttcgtctac acccattata agatgaatac
    20941 tctttggcta attccaccaa aaccgatgct gcttttttaa tacgataatt tttcgaccat
    21001 accgcggcat attgtgccac aggtaatgtt tcttcaactg gaatagtaat aaattgattt
    21061 gaaccaaaag gtgacgtcat atcacaagga attacagtta agaaatcagc attgagaaca
    21121 agattataaa ttgtcacgac tgagtcggtt ttaacgatgt tttcaatact gatgccattt
    21181 ctttgtaacg tagtaagaag ctcgctgtag taccccatat tggtttgtgg caacacccac
    21241 tgttcgttct tcaacgactc cagcgtggtg gtgccggtgc atgttcggga cttactggct
    21301 accagcacaa actcggactc gaacagcggc tcaacatgta aatcctgaag cttcatttct
    21361 gcacttaacg taccaatcgc aaaatccagt cgaccgtcgc ggattgccgg taagaacgaa
    21421 gacagttgcg cttcatacat agaaacctgc gctttcggga acacctcttt gaacttgttg
    21481 atcatccctg acataaaagt aaaaccaatc aatgaaggaa aaccaaatga gacttccacc
    21541 accgcctcag aagacatacc gcttatctca ttaaccatat ttttcatttc acgggtaatg
    21601 gattcggaac gggagagtaa caattgaccg gcaggtgtta atgttacacc ggtatttttc
    21661 cgcaccacta attccacacc aaaataatct tcaatatcgt taatgatttt actgacggcc
    21721 ggttgagtta accctaattc ttttgcagcc gagccgatag agccacttct aatgacttcc
    21781 tgaaagacta ccaggtgctg cgttttcgga agaagaatag tgctcataac gacctacgtt
    21841 aattacctca ttgacggcat gaagtgtatc aaaatgaaat gaacaggata tgtgcgacca
    21901 ctcacaaatt aactttcatt actttccagg gtatcgttat tacaattaaa tatctttaaa
    21961 tatcaacaag ttaaaacata aaaatcagca taaaccctga tttttatcaa aaaaaatgac
    22021 ggggataacc atattttatg gcgataacat cattcgttat gtggttaata caaaaaaggg
    22081 gctgagacca tatttcaagc aattaccgga taattaccag gcaaaatttg agttaaattt
    22141 aatgagcaag ttctcaaatt ttattataaa taaaccattt tcagcgataa atactgcggc
    22201 acgtcacatt ttttcacgct atttgttgga gaacaaacat ttattttatc aatattttaa
    22261 aatttcgaat acaggtattg atcatctcga acaattgatt aacgtcaact ttttctcttc
    22321 tgacaggacg tcattttgtg aatgcaatcg ttttccataa attcttctcc cctcataggc
    22381 gacgaatagc attttgtgtt gaggatcaca aaacaaataa ttgctggtcg ccgcgataag
    22441 gtcagacgaa gtcaacaagg ggaattttca ctgtgcttta catcagcgta ggacacagaa
    22501 tgttgcttcg tttactgtaa cgccgggtaa atgagcgttt tttgatagtg cgtaagtgtc
    22561 gtgcgaggaa aataagcatc tattattgtt tgtcatgacg acaaggcagg catcaatgaa
    22621 aggcttccca attgcacata tttttcaccc ttcaatcccg ccaatgcacg cagtggttaa
    22681 caatcacaat agaaatattg attattggac ggtaaaaaga aagtttgcag aaattgtctc
    22741 caccaatgac gttaaaaaaa tttacagtat aagtaatga
//
LOCUS       DAJVUJ010000056        22769 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.056, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000056 DAJVUJ010000000
VERSION     DAJVUJ010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22769
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.056"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            113..719
                     /locus_tag="OQQ48_001961"
                     /pseudo
     CDS             113..719
                     /locus_tag="OQQ48_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544752.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            822..1325
                     /locus_tag="OQQ48_001962"
                     /pseudo
     CDS             822..1325
                     /locus_tag="OQQ48_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
     gene            complement(1392..2849)
                     /gene="nuoN"
                     /locus_tag="OQQ48_001963"
     CDS             complement(1392..2849)
                     /gene="nuoN"
                     /locus_tag="OQQ48_001963"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HCS1952748.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQSLISIVRLAMPLM"
     gene            complement(2856..4385)
                     /gene="nuoM"
                     /locus_tag="OQQ48_001964"
     CDS             complement(2856..4385)
                     /gene="nuoM"
                     /locus_tag="OQQ48_001964"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HCS1952749.1"
                     /translation="MLLPWLILIPFIGGFLCWQTEHFDVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4549..6390)
                     /gene="nuoL"
                     /locus_tag="OQQ48_001965"
     CDS             complement(4549..6390)
                     /gene="nuoL"
                     /locus_tag="OQQ48_001965"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HCS1952750.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEILHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYFCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQSVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWQGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6387..6689)
                     /gene="nuoK"
                     /locus_tag="OQQ48_001966"
     CDS             complement(6387..6689)
                     /gene="nuoK"
                     /locus_tag="OQQ48_001966"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HCS1952751.1"
                     /translation="MIPLQHGLILSAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6686..7240)
                     /gene="nuoJ"
                     /locus_tag="OQQ48_001967"
     CDS             complement(6686..7240)
                     /gene="nuoJ"
                     /locus_tag="OQQ48_001967"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HCS1952752.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7252..7794)
                     /gene="nuoI"
                     /locus_tag="OQQ48_001968"
     CDS             complement(7252..7794)
                     /gene="nuoI"
                     /locus_tag="OQQ48_001968"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HCS1952753.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7809..8786)
                     /gene="nuoH"
                     /locus_tag="OQQ48_001969"
     CDS             complement(7809..8786)
                     /gene="nuoH"
                     /locus_tag="OQQ48_001969"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HCS1952754.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALIVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8783..11509)
                     /gene="nuoG"
                     /locus_tag="OQQ48_001970"
     CDS             complement(8783..11509)
                     /gene="nuoG"
                     /locus_tag="OQQ48_001970"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HCS1952755.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSLE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11562..12899)
                     /gene="nuoF"
                     /locus_tag="OQQ48_001971"
     CDS             complement(11562..12899)
                     /gene="nuoF"
                     /locus_tag="OQQ48_001971"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HCS1952756.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12896..13396)
                     /gene="nuoE"
                     /locus_tag="OQQ48_001972"
     CDS             complement(12896..13396)
                     /gene="nuoE"
                     /locus_tag="OQQ48_001972"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HCS1952757.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13399..15201)
                     /gene="nuoC"
                     /locus_tag="OQQ48_001973"
     CDS             complement(13399..15201)
                     /gene="nuoC"
                     /locus_tag="OQQ48_001973"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HCS1952758.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15295..15957)
                     /gene="nuoB"
                     /locus_tag="OQQ48_001974"
     CDS             complement(15295..15957)
                     /gene="nuoB"
                     /locus_tag="OQQ48_001974"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HCS1952759.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15973..16416)
                     /gene="nuoA"
                     /locus_tag="OQQ48_001975"
     CDS             complement(15973..16416)
                     /gene="nuoA"
                     /locus_tag="OQQ48_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HCS1952760.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(17047..17985)
                     /gene="lrhA"
                     /locus_tag="OQQ48_001976"
     CDS             complement(17047..17985)
                     /gene="lrhA"
                     /locus_tag="OQQ48_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HCS1952761.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            18904..20121
                     /gene="alaA"
                     /locus_tag="OQQ48_001977"
     CDS             18904..20121
                     /gene="alaA"
                     /locus_tag="OQQ48_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HCS1952762.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            20205..20803
                     /gene="yfbR"
                     /locus_tag="OQQ48_001978"
                     /pseudo
     CDS             20205..20803
                     /gene="yfbR"
                     /locus_tag="OQQ48_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
     gene            complement(20862..22694)
                     /locus_tag="OQQ48_001979"
     CDS             complement(20862..22694)
                     /locus_tag="OQQ48_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HCS1952763.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPVGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
BASE COUNT         5844 a         6273 c         5696 g         4956 t
ORIGIN      
        1 ttgtatagat ctaacaaccg taataaaaca aatactcata actcattata aataatgata
       61 aaataacgtc ttttattgat aatttatata tgggaaaaat tccttttgtt atattgtttc
      121 tgatttcact ccctctgatt atttttacca accctgggtc actgcattac caccaacgcc
      181 ccgacacgtc agtcctgagc aattagaaaa aacagttcgt tatcttacac aaactgtgca
      241 tccacgtaat gccgacaata ttgataatct gaataggtcc gtagagtata taaaagagtt
      301 ctttgtcagt agcggtgcca gagttaccgc gcagggcgtc cccgattggc tattacgact
      361 ctgcgcctgg tagccaggat tacctttacc cgcaatgtcc tggctttatc ccgatcgggg
      421 ggactttatt gcctggtcgg cagaatacag gatatcaacg ccgttcgtca ggtaaaaacg
      481 gcattgttat catcccggga tttatctgtt tattctatga atgccccatg gtttattccc
      541 ggtattgatt tctctgacca cctgaattat tggcaacacg atattcccgc cgtaatgatt
      601 actgacaccg ctttttatcg taataaacaa taccacttgc ccggtgatac agcagacaga
      661 ttgaattatc agaaaatggc tcagatggtg ttataacctt gttacacaac agtaaataat
      721 aaaaatttct cgaggcgggt ccagaaacag cgacatgtac attttatttt ccctcgtctg
      781 ttttaagatg cgaaacataa aattcaatgg aggaaaaata aatgggaatg attggttatt
      841 ttgcagaaat tgactcagaa aagattaacc aatctgcttg agtctactga aaaacctttg
      901 atggataata tacatgacac gctttcagga ttacgccgac tggatattga taaacgctgg
      961 gattttttac acttttgggc tgacaggcac ccctgctttt gatcccgcga agaatgatcc
     1021 gctgagtcgt gccgtgcttg gtgaacacag cctcgaagat ggcatagatg gcttcctggg
     1081 attgacctgg aatcaggaat tagccgcgac aatcgaccga ctggaatcac tggatcgaag
     1141 taagctgcgt aaacagtttt ccattaagcg actatgaaat ggagatttat ccgggagtga
     1201 ccttttcaga agagttagag gggcaactct ttgcctcgat catgcttgat atggaaaagc
     1261 tgattagcgc gtatcgacgt atgctgcgac aagggaatca tgcgctgacg gttattgttg
     1321 ggtgatggtg tcagagcgcc aaaaaaaacc gccgaacatg tcggcggttt tctgacttac
     1381 aaactaacag attacatcag cggcattgct aaccgcacaa tactaatcag cgattgtggc
     1441 catacaccca gcaccagtac caacagtgca gaaatcagca ccacgatacc gcccgcgctg
     1501 tactgccagt ttgacggtgc atcgcgaccc ggttgttccg gtgcgtgcag gtacaggctc
     1561 accgccacgc gcaggtagta gtagaggccg attgccgaac cgacaaccac ggcacccacc
     1621 agccaccaca agtgtgcctg gacaccgacc gccagcacgt agaacttacc gataaagccc
     1681 agcgtcatcg ggataccggc cagagacagc atcatcaccg tcatcactgc cgcgaggatc
     1741 ggacgatgcc agaacagacc gcggtaagag aacagggaat cagcatccgg gccacgatac
     1801 gggctggaca tcaggctgac cacgccgaac gcgccgaggc tgctgaacag ataaccggcc
     1861 aggtaaaccc ctaccgcttc catcgacatc tcgccggttt gcagcgcaat cagcgccacc
     1921 agcagatagc cgaggtgaga gatagatgag taaccgagca gacgtttgat gttggtctgg
     1981 ctcaacgcca tcaggttacc gaagatgatg gaggcaaagg cgataatcgc cagtactacg
     2041 cgaatcgctt cgctgtcacc caccggtgca tagaggaaca gacgcatcac cacaccgaag
     2101 atagcgattt tgctcgccgt cgccaggaaa gtggaaaccg gcgcaggcgc gccctggtat
     2161 acgtctggcg tccacaggtg gaacggcacc agagagagtt tgaagccgag gccaacaatc
     2221 atcatgccga aacctgccag caacagcggc tcgttgagca taccgtcgcc gaggttttta
     2281 cccagcgcga caaacgacag gtcgccagac tgcgcataca ccagcgccat accaaacagc
     2341 aggaaagaag acgctgcggc agaaaggatg gtgtatttga tactggcttc cagcgaacgt
     2401 ttctggcgga aagcgtaacc gaccaggcca aacagcggca aagagatcag ttcaataccg
     2461 aggaacagag acgccagatg gtttgcattc gccagcagga tcccgcccag cgcggcaatt
     2521 aacaccaaca ggtagaactc atccttgttg tcgttatagc cttcaagcca cgggtaggcg
     2581 aaagtacagg tggcgaggct cgccaacaat accagcccgg tgtaaagcat ggcgaaacca
     2641 tcaacgcgca tcagcggcgt aacgtccata gcgcccgcct ggccaacaaa ccagagcgaa
     2701 accagcgccg cgttaagccc aataaccgag agcgtagcgt tgaggaaatg attgcgtcgc
     2761 cacgcaatgg agagcatcac aaccaccacc gttaagccga cgatcagcaa cggtagcagt
     2821 gcgatcaggt tttgtggagt tattgtcatg gcgatttacg gccttgtagt agtaacggaa
     2881 ttaacaaacc actgctggat attgccaatc gcggagtgcg aggtatccag aatcggctgc
     2941 ggatagaagc ccagcagtac cagcagcacc accagcaaca ggatcataaa cagctcacgc
     3001 agcgacatcc ctggcagttc ctggctggca atctggcttt tcgctttacc gaagtaagcg
     3061 cgatgcaaca tcgccagcga ataaacagat gcaaagacca gcccaaaggt agagataacg
     3121 gtaatcaccg ggacaacctg gaagctgccg aacagaatca taaattcgcc gacgaagtta
     3181 ccggtgccag gcatcccaag cgttgccacc gcaaagaaca gcgacaatgc tggcagccat
     3241 ttcatcttgc tccacagacc gcccatcatg cgcatgtcgc gggtatggat acgttcataa
     3301 agctgaccac aaagaataaa cagacccgcc gccgacaaac cgtgcgcaat catctggatt
     3361 accgcgccct ggtaggccaa ctggctgccg gtgtagatag caatcagcac gaagcccatg
     3421 tgggaaaccg aggtgtaggc gatcagacgt ttgatatcgg tctgggcgaa ggccatccac
     3481 gcaccgtaga agatgccgat aacacccagc cacatagcaa ttggcgcgaa ctctgccgac
     3541 gcgttcggga acagcggcag ggagaaacgc agcaaaccgt aagcggcagt tttcagcaag
     3601 atccccgcga gatcaacgga accggcggtc ggagcctggg agtgcgcatc cggcagccag
     3661 ccatgcagcg gaaccaccgg cattttgact gcgaaggcga tgaagaagcc cagcattaac
     3721 aggtattcca caccactgga cattggcgta ttcagcagct cttcatagtt gaaggtccag
     3781 acgccggtcg cattgtagtg aacaaaaacc agcgccagga tggcaatcaa catcaccagg
     3841 ccactcgcct gggtgtaaat gaagaacttg gttgccgccg tgatacgcgt tttaccgtca
     3901 gaggctttat gcccccacag tgcgatcagg aagtacatcg gcaccagcat catttcccag
     3961 aagaagaaga acaggaacat gtcgatggca aggaacacgc cgataacgcc gcccaggatc
     4021 cacatcaggt tgaggtggaa gaagccctga tatttttcga tctctttcca cgaacagagt
     4081 accgccagca caccgagcag accggtcagc acgaccatca gcagcgacag accgtcaatg
     4141 gcgagatgaa tagagatacc aaaacgtggg atccacggca tgtcgaattc agactgccac
     4201 tgcggaattc cggcggattg cgtcagtgaa taaccgccct gcaaccacag ttgcagcgac
     4261 agcgccagcg tcaatcccat ggtgatcagc gcgatccagc gtggcacctt gacgtcaaag
     4321 tgttcggtct gccagcacag gaagccgcca ataaagggaa ttaatattag ccagggtagt
     4381 aacatggcga tctttattcc ttgtaaaagt cccgtcagga ccggattttc aacgaattct
     4441 cacgacagaa aattctcttc ggactggggg tagtgtcgga tgcgccgcga aggcagctta
     4501 tccgacctac gttcttatat gcccaggggc aatcccacaa tccttaactc aacgcagtac
     4561 catcaacagt gccagcacca cgaccgcacc gatgctcatg gatgccacat accagcgcag
     4621 ataaccgttc tcgcttaaca gcaggccttt acctgcaaag cgggaaagaa ccgccggaat
     4681 gttcatcatt gagttcagcg gatcgcgttt cagcaaccag gcaataccca gaaacggctt
     4741 gacgaacact ttgtcgtaca gccagtcaaa gccccaggcg ttgtaccacc aggtgcccag
     4801 cagacggccc ggcgcactgt tggcaatgga ggtcaccaga gtacgtttac cctgccacag
     4861 ccaggctgcc agcagaatgc cgaccaccgc gaccacgcca gaggtaattt ccagggtcaa
     4921 catgctgccg tgcgccagtt ccgtcgtttg cggaagcacg ctctgcagcg gcggtacaat
     4981 cagtgcgcca acgaaggtgg aaaggatcag cagcacaatc agcggcaggc tgtgagttac
     5041 ccctttcacg gcgtgagcgt gaatttgttc ttttccgtgg aagacgatga aaatcatacg
     5101 gaaggtgtag agcgaggtca taaacgcacc gaccagacct gccaccatca gattgatatg
     5161 accattcgcc atcgcacccg cgaggatctc atccttactg aagaagcccg cagtgaccag
     5221 cggtagtgcc gacagtgctg cgccgcccac caggaagcag aaataaacca gcggaataga
     5281 tttacgcaga ccgcccatct tgaagatgtt ctgttcgtga tggcaggcca gaatgacgga
     5341 accggatgcc aggaacagca gcgctttaaa gaacgcgtga gtcatcaagt ggaaaatcgc
     5401 cgcatcccat gcctgcacgc caagcgcgag gaacatgtag ccaatctggc tcatggtaga
     5461 gtaagcgaga acacgtttga tgtcggtctg taccagcgcg gcaaaaccgg ccagcagcag
     5521 cgtaaccgcc ccgacaatgc ccaccagatg cagaatttcc ggcgtcatca ggaacaggcc
     5581 gtgggtacgg gcgatcaggt agacacccgc ggttaccatg gttgcggcgt ggatcagcgc
     5641 ggagacaggc gtcgggcccg ccatcgcgtc ggcaagccat gtctgcaacg gcaactgcgc
     5701 agatttaccg accgcaccgc ccagcagcat cagcgtcgcc cacatcagca tgttattgcc
     5761 gtcagcaaag tgcgctggtg ccagttccac catttcgcgg aagttcaggg tgcccagttc
     5821 gttgtaaaga atgaacagcg cgaaagcgag gaacacgtca cccacacggg tcacgacgaa
     5881 cgctttcatt gccgctgcgc cattcttcgg atcggtgtaa tagaacccga tcagcagata
     5941 ggagcacagg cccacgcctt cccagccgag gtacatcagc agcaggttat cggcgagaac
     6001 cagaactacc atgctggcga tgaacaggtt ggtgtaggcg aagaagcgag agtagccctc
     6061 ttcaccgcgc atgtaccagg aggcgtacat gtgaataagg aaacccacac cagtgaccac
     6121 cgagagcatg gtcagcgaca ggccgtccag caccaggtta aaaccgatgt taaagtcgcc
     6181 taccgacatc cacgtccaca gcggctggct gtatgcctgc tcgccgttag cgaagaaatc
     6241 aacgccgata aaggcggtta ccagcgccgc caggcccaca gagccaacgc cgacgatcgc
     6301 cgaaacgttt tcagaccagc gcccacggga gaacgccagc aggacgaagc caatcaatgg
     6361 caaaataatg gttaaggcaa gcatgttcat ccgcgcatct cacttactga atcgatgttc
     6421 aggttctggc gacgacggtg aagttgcagc agcagcgcaa ggccgatact cgcttctgcc
     6481 gccgcgaggc tgatggcgag aatgtacatc acctgaccgt cggtctgacc ccagtagctt
     6541 ccggcgacca caaaggccag cgcggaggcg ttaatcatga tttccagacc aatcagcata
     6601 aacagcagat tgcgacggat aaccagaccg gttaagccaa gaacgaataa gattgccgag
     6661 aggatcagtc catgttgtaa ggggatcatg cgtgctcctc cgtttttctt ttcgcgctgt
     6721 cgtctttacg attgctcagc acttcacccg cacgctcttc acgaccgacg tggaaggcca
     6781 caaccagacc cgcgagcagc agcatagaag ccagttccac cgccagtacg taaggcccga
     6841 acagcgtaat acccactgct ttagcactga ttggcgtacc gtcgataccc tgatcattaa
     6901 caccaaggat ggcgtaaaca atcaccacca gcatgatggc cgacaaaatt gccggaccaa
     6961 tccacacctg cggtttcagc cactggcgtt cctgttcgat ttctgaaccg cccaggttga
     7021 gcatcatcac cacgaacacg aacagcacca taatggcacc cgcgtagacg ataatttcca
     7081 gcgcaccggc gaagtaagcg cccagtgaga agaacacccc ggaaatcgcc agcagcgaaa
     7141 taatcaggta cagcagtgcg tgtaccggat tggtatgggt gatcactcgc aaggtcgcaa
     7201 gtatggctat caggccacag atataaaaag cgaactccat tgcccctctc cttacggtaa
     7261 caggctcttg acgtcgatag gcttggcttc gttctctgct tcgcccttat ctttgccgtc
     7321 gattgccata cctgccatcc ggtagaagtt atattccggg tatttgcccg gaccggagat
     7381 cagcagatcc tctttttcgt aaaccagatc ctggcgcttg tattccccca tttcgaaatc
     7441 cggggttaac tgaatcgccg tggtcggaca ggcttcttcg cacagaccac agaaaatgca
     7501 gcgtgagaag ttgatgcgga aaaattccgg gtaccagcga ccgtctttgg tttctgcttt
     7561 ttgcagcgag atacagccga ccgggcaggc taccgcgcag aggttacagg ctacgcaacg
     7621 ctcttcgccg tccgggtcgc gggtcagaac gatacgacca cgataacggg gcggcagata
     7681 gaccggctct tccgggtaca ttcgcgtttc gcgtttggcg aacgcgtgca ggccgatcat
     7741 ccagatacta cgaacctggg tgccgaaacc tactaacaat tcttttaagg tcatggtctt
     7801 attgcccctt attgcgcctg ccagagaatg acagccgccg ttaccagcaa gttgatcagc
     7861 gtcagcggca ggcagatttt ccagccgaag gacattacct ggtcataacg cggacgcggt
     7921 aacgacgcac gaatcaaaat gaacatcatc ataaagaacg cggttttcaa cgcgaaccag
     7981 atgaatggcg gtaacaacgg gccttgccag ccaccgaaga acagcgtcac aatcaatgca
     8041 gagatggtca caatcccgat gtactcaccc acgaagaaca gaccgaactt cataccggaa
     8101 tattcaatgt ggtaaccatc cgccagttcc tgctcggctt ccggctggtc aaacgggtga
     8161 cggtgacata ccgccacgcc cgcgatggca aaggtaataa aaccaaagaa ttgcgggata
     8221 acgttccaca catgcgcctg gctgttgacg atgtcggtca tgttgaatga accggcctgc
     8281 gccaccacgc ccatcaggga aagcccgagg aacacttcgt agctcagggt ctgcgcagaa
     8341 gcacgcatcg cacccagcaa cgagtatttg ttgttacttg accagcccgc aaacagcacc
     8401 gcgtaaaccg ccagacctgc catcatcagg aagaacaaaa tcccgatgtt caggtcggca
     8461 accacccaac ccggactgac tggcacaatc gcaaaggcca gcagcagcga ggtaaaggca
     8521 atcatcggtg ccagggtaaa gatgacgcga tccgagaatt tcgggatcca gtcttcttta
     8581 aagaacattt tgatcatgtc cgcaaccagc tggagcgaac cgccccagcc aacacggtta
     8641 ggtccataac ggttctggaa cagacccagc aggcgacgtt cgccaaagct catgaatgcc
     8701 ccacaggtga caaccaccag caggatcacc accgctttga ggatggtcag caggatctca
     8761 atcagttccg gtgatatcca actcattgtt gtgcctcctt gagatcctca agatgcgcgc
     8821 cagccagcac cggagcaatg ccggacatac ccatcggcaa gcccacctgc cctgccgtca
     8881 gtccttcggc gatttcaacc ggcagcgtga ccgtgttgcc atcgtaacta aaggagacgc
     8941 gtgtacctgc gttcacaccc aacttcgcgg catccgctgg gttgagtttg atgtacggct
     9001 gcggcatacg gctctggaag accggagcac gctgtgacaa ttcatcgctg ccaaacaggt
     9061 ggtaatacgg cgcaatacgc catttcccgt cctgcggctg gaagcgtgcc ggtacgctgg
     9121 tgaagtaatc cagaccattc tcgctggttt caaacagacg cacgcccgga tcgccaaagc
     9181 gcagtttgcc gcccacttcg tcctggaatt tgttccacgc ctgcggggag ttccagcccg
     9241 gtgcccaggc aaacggcact tgcgaacggt gcgcggtcgg ctggttgtta ccttccatcg
     9301 agaaggtgaa catggtgtca atatcctgcg gctgacgcgg ctcatgaacg ctgatattgg
     9361 cgcgcatggc ggtacgaccg ctgtaacggt gcggttcacg ggccagtttc tgcccacgaa
     9421 tacggaatgt cgcatccggc gcagcatctt tgatacctgc cagttccggg attttcgcca
     9481 caacagcgtc aatcacatgg tcgagctgcg tccagtccac ttcacggctc agcagggtgc
     9541 tgtgcagcga gtgcagccag cgccagcttt ccagcatgac agttttgctg tcgtaataag
     9601 ccggatcgta aacctggaag aaacgttggg cgcgaccttc attattaatt accgtaccgt
     9661 cgctttcagc aaagctggcg gcggaaagta ccagatgggc gttttccatg atcgctgtac
     9721 gctgatggtc aaccaccatc accagcggtg ctttagccag cgcagcattc acgcgggtag
     9781 cagaagcgtg acgatgcaga tcgttttcca acaccaccac cgcgtcggcg cgtccggttt
     9841 ccagttcggt taacgcttct tcaagcgaac cgccacccat aatgcccagc cccatgctgt
     9901 tgacggaacg ggcaatcatg gtgataccga cgtcagcgcc gcgacctttc agggctttcg
     9961 cgacgttagc cgccgcctga atcacctcta agctaccggc gttcgtcccg gagataatca
    10021 acggtttctt cgcacctgcc agtgcctgca cgatgacgtc gattttgctt tgcagctcag
    10081 gttcgatacc gtcaaccgct ggtgcagagt tatccagcgc atgggcgatg gcaaaaccta
    10141 aacgcgcctg atcttcaacc ggtgcgcggt aagtccacgc cgcgatatca tccaaacggg
    10201 tgtcatcaac gttggtaaca aacagcggat gcttcgcacg ttgaccgatg ttaaggattg
    10261 ccgcaatctg ccagtcagcc actttctgtg ctgccgccat ttcgcgcgct ttacctttca
    10321 ccgcctgacg cactgccagc gcgacgcgcg cgccggtctg ggtaacgtct tcgcccagca
    10381 ccagtaccgc atcgtaagat tcgatttcgc gcagagccgg agtataaatg ccgccttcac
    10441 gcagcacttt cagcgccagt tgcagacgtt cctgctcacc gtgagcgata ccggtgtaga
    10501 agttttcttc gcccaccagt tcacgcagtg caaagttgct ttccacgctg gcacgcggag
    10561 aaccaatacc gatcactttc ttcgactgac gcagaatatc tgccgcgccc tgcattgcct
    10621 gttcggcgtt gagggtaatg aaatcatcgc cacgacgctg tactggctga cgcggacgat
    10681 ccttcaggtt gacgtaaccg taaccgaaac gaccacggtc gcagaggaag tagtggttta
    10741 ccgtaccgtt gtaacggttt tcgatacgac gcagttcgcc gtaacgttca ccggggctga
    10801 tgttacagcc gatggaacat tgctggcaga tgctcggcgc aaactgcata tcccatttac
    10861 ggttgtaacg ctcggagtgc gttttgtcgg taaatacgcc ggtcgggcaa atttcgacca
    10921 ggttaccgga aaattcgctt tccagcgtgc cgtcttccgg gcgaccgaag tagacgttgt
    10981 cgtgcgcgcc ataaacgccc agatctgtac cgtcagcgta atctttgtag taacgcacac
    11041 agcggtagca ggcgatgcag cggttcattt cgtgagatat gaatggcccc aaatcctgat
    11101 tacggtgggt acgtttggtg aaacggtagc gacggaagct gtgtccggtc atcacggtca
    11161 tatcctgaag atggcagtta ccgccttctt cacataccgg acagtcgtgc gggtggttgg
    11221 tcattaacca ctcgaccaca ctttcacgga actgtttcgc ttcttcgtcg tcaatggaaa
    11281 taaaggtgcc atcggaagcc ggtgtcatac aggacatcac caggcgacca cgcgtgtctt
    11341 ccgcgttttg gtattgcttc accgcacact ggcggcaagc accgacgctt cccagcgccg
    11401 gatgccagca aaagtaagga atatcaaggc ccagagacag acaagcttcc agcaggttgt
    11461 ccgctccgtt gacctcgtat tctttgccgt ctacatgaat tgtagccatt agcatgcttc
    11521 cagttttctc ggtcagggac cgagcgttaa tcgaaattcg gttaccagcg ctctttcagc
    11581 aggttcggct gaatcccatt aatcaaatgg gtattgctga acggctgttt gattcccgcc
    11641 tcaaattctt cgcggaaata tttgatggcg ctctgtaacg gctccactgc accaggtgcg
    11701 tgggcacaga aagttttacc cgggcctaag aatcgacaca gttgctcaag tgtttcaata
    11761 tcgcccggct ggccttcgcc acgctccagc gcacgcagaa ttttcacgct ccacggcaga
    11821 ccgtcgcggc acggcgtaca ccagccgcag gactcacggg cgaaaaactc ttccaggtta
    11881 cgcaccagcg acaccatgtt gatctcatgg tcaaccgcca tcgccagcgc cgtacccaga
    11941 cggctgcccg ctttaccgat actttcgaat tccatcggca gatcaaggtg cgcttcggtc
    12001 aggaagtcag tccccgcgcc gcctggctgc caggctttaa atttcagacc atcacgcata
    12061 ccaccggcgt aatcttcgag gatctcgcgt gcggtggtgc cgaacggcag ttcccacagt
    12121 cccggatttt tcacccgacc ggagaagccc atcagcttgg tgccagcatc tttacttttc
    12181 gagatgttct gataccactc cacgccgtta gcgaggatcg ccggaacgtt acacagggtt
    12241 tcgacgttgt tgacacaggt cggtttgccc catacgccgg aggttgccgg gaaggggggt
    12301 ttcgagcgtg ggttagcacg acgcccttct agggagttga ttaacgctgt ttcttccccg
    12361 cagatgtagc gccctgcccc ggtatggacg aacagttcga agtcgaaacc tgttcccata
    12421 atgtttttgc caagcaagcc cgcttcagtg gcttcggcaa tggcgcggcg cagatttact
    12481 gccgcttcga tatattcgcc acgcaggaag atgtagccac ggtaagcttt cagcgcaaac
    12541 gcggagatga gcataccttc caccagcagg tgcggcagtt gctccatcaa caggcggtct
    12601 ttataggtgc ccggctccat ttcatcggcg ttacacagca ggtaacggat gttcatggat
    12661 tcgtctttcg gcatcaggct ccacttcagg ccagtagaaa agcccgctcc gccgcgccct
    12721 ttcagaccag cgtcttttac ctgattaacg atttcgtccg gagacagccc ggtcagcgcc
    12781 ttacgcgccc cttcgtaacc gtttttgctg cggtattcgt ccagccacac tggctgtttg
    12841 tcatcgcgca gacgccaggt cagcggatgc gtttcgggag tacggataat gtttttcatt
    12901 tataccgctc cagcagttca gggatcgctt ccggggtcag atgcgcgtga gtgtcctcat
    12961 cgatcatcat gtttggccct ttatcacagt tccccaggca gcaagttggc agcagcgtaa
    13021 agcggccatc aaatgtcgtt tgccctggtt tgatgttcag ctttttctcg agcgccgcct
    13081 gaataccctg atacccgttg atatgacaga ccacgctgtc gcaataacgg atcacatggc
    13141 gaccaaccgg ctggcggaag atctgactgt agaacgttgc cacaccttcg acgtcgcttg
    13201 ccggaatacc cagcacatcg gcgatcgcgt ggatcgcacc atccggcacc cagccacgct
    13261 gcttctgaac gattttcagc gcttcaatgg acgccgcacg cgggtcttcg tagtggtgca
    13321 tctcgtgctc aatcgcttca cgctctgccg cactcagctc aaaagcctcg gtttgtggtt
    13381 gttgattctc gtgcataatt agcggtccac atctgacata acaaaatcga tactgcccag
    13441 ataaacaatc aggtcagaca ccaggctgcc gcggatcgcc gccggaattt gctgcaaatg
    13501 cgcataactc ggcgtgcgga tacgagtgcg ataactcatg gtgctaccgt cgctggtcag
    13561 gtagtaactg ttgatcccct tggtcgcctc aatcatctgg aaagattcat tggcaggcat
    13621 caccggcccc cacgacactt gcaggaagtg ggtgatcagg gtttcgatat gttgcagcgt
    13681 gcgctctttc ggcggcggcg tggtcagcgg gtgatccgct ttgaacgggc cttccggcat
    13741 gttgttgagg cactgctcaa gaatgcgcag actctggcgc agctcttcca ctttcagcat
    13801 tacgcgggtg tagcagtcag aaacaccacc accaaccggg atttcaaagt cgaagttttc
    13861 atagccagaa taaggacgcg ccttacgcac gtcgaagtcg atcccggtag cacgcaggcc
    13921 cgcgccagtg gtgccccact ccagcgcctc tttcgcgcca taggcggcaa cgccctggga
    13981 acgacctttc agaatggtat tttgcagcgc cgctttctcg taagacgcca gacgtttcgg
    14041 catccagtcg aggaactcac gcagcaggcg atcccagccg cgcggcaggt cgtgcgctac
    14101 gccgccaata cggaaccacg ccgggtgcat acggaaaccc gtgatcgctt ccaccagatc
    14161 gtaaattttc tgacgatcgg taaaggcgaa gaacacgggc gtcattgcgc cgacgtcctg
    14221 aataaaggtc gagatgtaca gcaggtggct gttgatacgg aacagttcgg agagcataac
    14281 gcgaatgacg ttaacgcgat ccggcacggt gatcccggcc agtttctcta ccgccagcac
    14341 gtaaggcatt tcgttaacgc agccgccgag gtattcaata cggtcggtat acggaatgta
    14401 gctgtgccag gactggcgct cgcccatttt ctccgcacca cggtggtggt agccgatgtc
    14461 tggtacgcag tcgacaatct cttcgccgtc gagttgtaaa acgatacgga aagccccgtg
    14521 cgccgacggg tggttcggac cgaggttgag gaacatgaag tcctcgtttt cggtgccgcg
    14581 cttcatcccc cactcttccg gtttgaaggt cagggcttcc atctccagat cctgtttggc
    14641 tttggtcagc tcaaacggcg agaattcggt agcgcgcgcc gggtaatctt tacgcagcgg
    14701 gtgacctttc caggtttgcg gcatcatgat gcggcgcagg ttcgggtgac cgtcgaaagt
    14761 aatgccaaac agatcccagg tttcacgctc ataccagtta gcgttcggga acagtttggt
    14821 gaaggtcggt acgtgcaggt cgttttctgc cagcgccacc ttcagcatga tgtcgcggtt
    14881 acgatcgata gaaatcagat ggtagaaaac ggaaaaatcc gcggcaggta acccttcgcg
    14941 gtgtgtgcgc agacgttcgt ccatgccgtg taagtcaaac agcatgacgt aaggtttcgg
    15001 cagtttcttt aagaaatcgc caacttccag taattgttca cgcttgatcc acacaacggg
    15061 aaccccggtg cgagtcgcct gaacagtaaa ggcatccggc ccaaaacggt tgcgcagttc
    15121 gccaattacc ggatcatcaa gatgatcgcg ggtctgccag gcgggttctt gcgcggttaa
    15181 gtcggtcata ttgttcacca ttgcaaatgg tccgtggtga ctgtcggcgc agtaagcttc
    15241 gcgaaataga tttaggaata agcgaagaaa aatccctttg ccgacaggcg caaattaaat
    15301 ctcgtcaggt gtacgcaggt tggttacggc aatgcgttca ccgcgcttgc gttcgcgctc
    15361 tgattgcata ttggcgcgat aaacgccctg atcgccaacc acccaggaga gcggacgacg
    15421 ttctttgccg atagattcct gcaacagcat cagtgcctgc atgtacgctt caggacgcgg
    15481 cgggcagccc gggatataca catcaaccgg gatgaattta tcgacgccct gcacaacgga
    15541 ataaatatcg tacataccac cagagttggc acaggcaccc attgagataa cccattttgg
    15601 ttccagcatc tggtcataca gacgctgaat aaccggtgcc attttggtaa agcaggttcc
    15661 tgcaaccacc atcaggtcag cctgacgcgg cgaagcacgc aatacttctg cgccaaaacg
    15721 cgccacgtca tgcaccgcgg taaacgaagt caccatctca acgtaacagc aggaaagacc
    15781 gaagttatac ggccaaattg agtttttacg accccagtta accatgtcat tgagcttgcc
    15841 cataaacacg tttttgttaa cttcttgctc cagagggtcg gttacgatct cctgcttttg
    15901 cagggggtaa cggtcgttct caccgttggg atctatgcgg gtgagcgtat aatccatctt
    15961 aatgcctcgc ggttagcgtt gacgattagc gatactgttc gtttccgggt tcatacgctc
    16021 gcggcgtgaa cgcgcgggcg tccagtccag cgcgccaata cgcaccagat aaaccagacc
    16081 tgccagtaac acaaaaataa aaattgcagc ttccacaaag cctacccagc cgctttcgcg
    16141 gatagaggtt gaccatgcga acagatacag cgcttcaacg tcgaagataa cgaagaacat
    16201 ggccaccaga taaaacttgg cggacaggcg taagcgggcg gagccgaccg agtcgatacc
    16261 ggattcaaac ggcacgtttt tcgacctcgc gcgtgcgcga ccgcctaaaa accaaccacc
    16321 taccagcatt aggcaacaca ggccaatggc aacgataaga aagatagcga atgcccagtg
    16381 atgagcgatg acttcagtgg atgttgacat actcattgct tactcatcaa aagtagcgcc
    16441 agattcactg ctcttcacgg cagatggacg ccacatcgat tcatggggag gaataaaaaa
    16501 aaccttacaa tcactgtaga aattctttta tacagctaat tgatgtggtc ttttactcct
    16561 ttctataacc ttttgtcaac tttaacaaaa gtttcttcac attagtttac ataatatcaa
    16621 caccattagc atttaatgcc ctttcacccc agatccttga cgactccagg ataattagat
    16681 gttgttgaat cgtgtccgtt gtgaagcaat ggaaaaaata cgggtctatt ttgacaggaa
    16741 tttgtgtcga ttcctccccc caaaagagag tattttcttg atctgtgaca cgcttttgtc
    16801 attccataac aaaaacgcag caacaaattt acgtattttt taacatcatt gtagcaggtg
    16861 atttttttca ggcgattatt tgtgcgttcg ggacgtgaat ctctggtgat tgaaaaatga
    16921 acagttttgt acgttctgca ctatgcgagg aaggattttt actaaaaaaa agccgctggg
    16981 gtttaaaaca cccccagcgg ctcgtttttt acactattgt ctcaggaatt atctatcgtc
    17041 cgtcgattac tcgatatccc tttcaatcaa caatgaatca tccccttccg gagcagacat
    17101 tgggctgtat tgccacggat tatggtagct ctccatcgcc tgatagatca cctgggccag
    17161 ctcattatta ctggacgggt catagcacag caaatattcg gtatcaggta gcggcggtaa
    17221 gccatctact ccactcagca cacgcaggtc cgggctcatc atctcaaccg gccttgccgt
    17281 cacgccaaga ccggctttca ctgccgcacg aacggccgga agcgtcgagg cgacataagc
    17341 cagtcgccat ggaatatctg ctttattaag cgttgccagc accatatcgc gaaacggact
    17401 tggatcatcc agcaatacaa gagggatcgg ctcgcctttt tgcaatacgt attccgctgc
    17461 gcagtaccag tgtgttggcg aggtacgcag gttcaatgcc ttaaacgctg aaggacgatg
    17521 ggtggttacc atcaaatcca cttcctggga ttccagcatt tctgccatat aggcattacg
    17581 tttaacgcgg acatccagcg ctaatttcgg ataaaccgaa ctcacgcgat ttaacaggaa
    17641 aggtaagatc gtatcggcag attcatctga agcaccgata gttaacacgc cctgaagatt
    17701 actgaacatt aatgatgagc aggcctcatc attaaaacgc aggattttcc tggcgtaacc
    17761 aagaagttga atgccatgtt cagttaacag tttgttgcga ccgtgacgag cgaacagttc
    17821 tttcccaacg agttgttcca gacgctgcat ttgctgactt acggcggact gagtacgaca
    17881 cacagcggca gctgcggcag caaaagtgtt cagatcggca acagcaacaa atgttctcag
    17941 cagatcgagg tcgaggttaa ttatcggacg atttgcactt atcatatatt atcacttact
    18001 ggcggctcat actgagctgg ttaatgctgt gcacacacaa acaagcaatt ccatttgtaa
    18061 tgtgcctccc tggcagtttc atccggaacc cggacgaaag taaaaatgca tatgagttgc
    18121 actaaaaaag cgactcacat tgttccgtta taatgcctga agtagatcac agaatatatc
    18181 ttcagggatc gcatatctat taagttactc actcttttct atttatgaca tgcgcgtgtt
    18241 tgtataaatg taaatgtgag tccttgttcc actctcgtgc agcatcgctg gtcatacgcg
    18301 aacacgtaca aacagcaatg gtgaggcggc atcaagagca gggatccgtc atttatgcga
    18361 gcattttacc ccaaaaacct tttatttata agggccattg cgaatcatct gatgcaaagt
    18421 tatgttatgt taggcaaagt aatcttctat tattaataag cacatcaaaa cctttttgaa
    18481 tattaaataa taattaatta gcatcatcct cattcattaa ttccgcttaa caatagtttc
    18541 acaccttgcc ccattacgac acctcggaaa tcatcacgct gttaaactct ccgtaatgcg
    18601 gaaatatcat catcgcgttt cattagtgaa ttcttaaatg aggcattttc acacaattat
    18661 cttacagata aaaaaaccag acttacaatt aagaatcaga acaattcact atataacatt
    18721 gcatgtaaag catatacacc tcattatttt gtcatcatta agttattaac agcacaatcg
    18781 agccttcccc tctggcaaaa tcttattctg cagaccttca aaacaccgtc ctgggggagt
    18841 acattgttct aagctgactt ccacggcagg gagtggcgat aacagcaaaa aaggtcaaga
    18901 ttcatgtccc ccattgaaaa atccagcaaa ttagagaatg tctgttatga catccgtggt
    18961 ccggtgctga aagaagcaaa acgcctggaa gaagaaggta acaaggtact gaaactgaac
    19021 atcggcaacc cagccccgtt cggttttgac gcgccagatg aaatcctcgt tgacgtgata
    19081 cgcaacctgc ctaccgctca agggtattgc gattccaaag gtctttactc cgcgcgtaaa
    19141 gccatcatgc agcactacca ggctcgtggc atgcgtgatg ttaccgtgga agatatttac
    19201 atcggcaatg gtgtatcgga gcttatcgtt caggcaatgc aggcattgct gaacagcggg
    19261 gacgaaatgt tggttcctgc accagattac ccactatgga ccgcggcggt ttcgctttcc
    19321 agcggtaaag cggtgcatta tctttgcgat gaatcctctg actggttccc ggacctcgat
    19381 gatattcgcg ctaaaattac gcctcgtacg cgtgggatcg ttattatcaa cccaaataac
    19441 ccaaccggcg cggtatattc caaagagctt ttaatggaga ttgtggagat tgcacgtcag
    19501 cataatctca ttatcttcgc cgatgaaatt tatgacaaaa ttctttacga cgacgctgag
    19561 catcactcaa ttgcgccgct ggcacctgac ctgctgacca ttacctttaa cggactgtcg
    19621 aaaacgtacc gcgttgcagg cttccgtcag gggtggatgg tgttgaacgg gccgaaaaaa
    19681 cacgccaaag gctacatcga aggtctggaa atgctggctt caatgcgcct gtgtgctaac
    19741 gttcctgcgc aacacgccat tcagaccgcg ctgggtggtt atcagagcat cagtgaattt
    19801 attacccctg gcggtcgtct ttatgagcag cgtaaccgcg cgtgggaact gatcaacgat
    19861 attccgggcg tttcctgcgt gaaacctcgt ggtgcgctgt atatgttccc gaaaatcgac
    19921 gccaaacgct ttaacattca cgacgatcag aaaatggtgc tggatttcct gttgcaggaa
    19981 aaagttctgt tggtgcaagg gacggcattc aactggccgt ggccggatca cttccgcatt
    20041 gtcacgctac cgcgtgtcga tgatatcgag ctgtctttga gcaagttcgc gcgtttcctt
    20101 tctggttatc atcagctgta atcttaattt cactgccgga gattgcatcc ggcagcgtta
    20161 tcccgccaca atgacctgat gatgtcatca tacgtaaggt cactatgaaa cagagccatt
    20221 tttttgccca tctctcccgc ctgaaactca ttaaccgctg gccgctaatg cgcaacgtgc
    20281 tgacggaaaa tgtgtccgaa cacagtttgc aggtagcgat ggtcgcccat tcgctggcag
    20341 ctatcaaaaa tcgaaaattt ggcggtaatg tcaacgccga acgtatcgct ttactggcga
    20401 tgtaccacga tgcctcagaa gtgccaccgg cgatctccct actccggtga aatacttcaa
    20461 ttcgcaaatc gctcaggagt acaaggctat tgaaaaaatc gctcagcaaa aactggtcga
    20521 tatggttccg gaagagctgc gggatatctt tgcgccgtta attgacgagc atgcatatag
    20581 cgatgaagaa aaatcgctgg tgaaacaggc agatgcactg tgtgcatatc tgaaatgtct
    20641 ggaagaactc gcggccggaa ataatgaatt cttgctggca aaaacgcgac tggaagcgac
    20701 gcttgaagcg cgtcgcagcc aggagatgga ctacttcatg gaagtatttg ttcccagctt
    20761 ccatctttcg ctcgatgaga ttagccagga ttcaccgctg taagcagccg gagtctgcgt
    20821 cgcatcaggc aataagcgcc ggatgcgaca tcaggctctt gtcaaaacgg aaacagcatc
    20881 gggatcatca ccacacaaac cgccatcacg ataatggtga acggtacccc caacttcaca
    20941 aagtcactga agctgtaatt tcccggacct aaaaccagtg tgttaacagg tgaagaaacc
    21001 ggtgtcataa aggcggcgga tgctgccatc gccacgacca tcgcgaatgg ataaggcgac
    21061 acccccatcg ttttggcagc agccagcgca atcggagcca tcaacaccgc cgtcgcggta
    21121 ttagagataa atagcccaat aaccgccgac aagacaaaca aacaccccag catcatatgt
    21181 ggcccgtaac caccgccaat gtccatcagc cctttcaccg ccagcgcgac acctcccgtt
    21241 ttctgtaatg ccacagcaaa cggcatcatc ccaacgatca aaataatgct cggccagtga
    21301 atggatttat aggcgctttc agcatctata cagcggaatt tccccatcag caggcaggcg
    21361 atgatagcgg caacaggatt aggaatttca tctgtcagca ttaacgccac cattagcacc
    21421 agacagaaaa tggcatgggg tgcctggctg tgcgcgggcg atgcttcact cacctcttcc
    21481 ggtaagttca gcgctacgaa gtcgcggccc tgtttggcca gcataccgat cagtttccag
    21541 ttacccacaa ccaggatgat atcgcccagc agcagaggct catccgccag cgaaccttcc
    21601 agcgccacgc cattgcgctt tagccccacc acattcagtc cgtagcgggt acgaaaacca
    21661 atttcgcgca ccgatttacc aatcagttct gactcaggaa ttaatgaaat ctctgccatg
    21721 cccacatcaa gggcctggtc agaaaaatac tcgccgcgca gtaccatcgg ctccagcaat
    21781 tgctcactac aaaattgccg gagatcgaca tcagccgcag acatatcaat aagcaaaacg
    21841 tcacgcgcgc gaaattcaga aaccccatta acgttcacga taacgcgacg aaaacgccgc
    21901 cagcgttcaa caccgatgac gttagcgcca taacgctcac gtaatttgag atcatccagc
    21961 cgttgaccaa tcattggcga tccggggcga atagccagac gtcgcgcacg cccggtcagt
    22021 cgatattcac ggataagatc gcgaaaggtt cgacgcgtcc agccttcgcg ctgcggggtc
    22081 tgggtatccc ctttcagcat gaaacgcatc actaacatat acaaaatacc cagcaccagc
    22141 acgaccaggc caacaggtgt tacgctaaag aaactgaagc cgtgatagcc ttcacgcagc
    22201 aattcactgt tgactaccag gttcggcggc gtcgccacca gcgtcatcat gccgctaatc
    22261 agcccggcaa aacttaacgg catcatcaga cgtgacggcg aagtttgcat acgcatggca
    22321 acgcttaaca ccacgggaat aaagatagcg acaacgccgg ttgaactcat aaacgcgcca
    22381 agccccgcga cggtcagcat caacaaaacc aacattttga tttcactgtt gcccgccact
    22441 ttgaccagcc atgttcccat tacggtggca acaccggtac ggaccaaacc atcgccaata
    22501 ataaacaagg cggcaatcag gacaacgtta ggatcagaaa agccggaaaa tacttcgggg
    22561 accgtcagcg ttccgcttaa tgcaaacgcg acaataacaa acaaagcgac cgcatccata
    22621 cgcactctgc ccgtcgcaaa caagacgatg gcaacggcca gtaatgaaag aacccaaatc
    22681 aattcaccgt tcacaactta tccttgttaa ttgaggggga tgacttgatt ctgccataaa
    22741 aaagccccga cgagacgggg ctaaatcat
//
LOCUS       DAJVUJ010000057        22564 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.057, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000057 DAJVUJ010000000
VERSION     DAJVUJ010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22564
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.057"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(<1..238)
                     /locus_tag="OQQ48_001980"
     CDS             complement(<1..238)
                     /locus_tag="OQQ48_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCS1952764.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGICTGEGLEI"
     gene            complement(229..699)
                     /locus_tag="OQQ48_001981"
     CDS             complement(229..699)
                     /locus_tag="OQQ48_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCS1952765.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(884..1678)
                     /gene="thyA"
                     /locus_tag="OQQ48_001982"
     CDS             complement(884..1678)
                     /gene="thyA"
                     /locus_tag="OQQ48_001982"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCS1952766.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYAPHPGIKAPVAI"
     gene            complement(1685..2560)
                     /gene="lgt"
                     /locus_tag="OQQ48_001983"
     CDS             complement(1685..2560)
                     /gene="lgt"
                     /locus_tag="OQQ48_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCS1952767.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(2711..4957)
                     /gene="ptsP"
                     /locus_tag="OQQ48_001984"
     CDS             complement(2711..4957)
                     /gene="ptsP"
                     /locus_tag="OQQ48_001984"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCS1952768.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGLPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPILLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(4970..5500)
                     /gene="rppH"
                     /locus_tag="OQQ48_001985"
     CDS             complement(4970..5500)
                     /gene="rppH"
                     /locus_tag="OQQ48_001985"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HCS1952769.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNTSAYRRKRG"
     gene            6185..6874
                     /gene="mutH"
                     /locus_tag="OQQ48_001986"
     CDS             6185..6874
                     /gene="mutH"
                     /locus_tag="OQQ48_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HCS1952770.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWTPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            6943..7656
                     /locus_tag="OQQ48_001987"
     CDS             6943..7656
                     /locus_tag="OQQ48_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCS1952771.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            7794..8012
                     /gene="ygdR"
                     /locus_tag="OQQ48_001988"
     CDS             7794..8012
                     /gene="ygdR"
                     /locus_tag="OQQ48_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HCS1952772.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            8120..9160
                     /locus_tag="OQQ48_001989"
     CDS             8120..9160
                     /locus_tag="OQQ48_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HCS1952773.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPSIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKTAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(9192..10385)
                     /gene="lplT"
                     /locus_tag="OQQ48_001990"
     CDS             complement(9192..10385)
                     /gene="lplT"
                     /locus_tag="OQQ48_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HCS1952774.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNSTPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(10378..12537)
                     /gene="aas"
                     /locus_tag="OQQ48_001991"
     CDS             complement(10378..12537)
                     /gene="aas"
                     /locus_tag="OQQ48_001991"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HCS1952775.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDASRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTLDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            12926..13957
                     /gene="galR"
                     /locus_tag="OQQ48_001992"
     CDS             12926..13957
                     /gene="galR"
                     /locus_tag="OQQ48_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HCS1952776.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(13964..15226)
                     /gene="lysA"
                     /locus_tag="OQQ48_001993"
     CDS             complement(13964..15226)
                     /gene="lysA"
                     /locus_tag="OQQ48_001993"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCS1952777.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSVPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRSLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            15348..16283
                     /gene="lysR"
                     /locus_tag="OQQ48_001994"
     CDS             15348..16283
                     /gene="lysR"
                     /locus_tag="OQQ48_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HCS1952778.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     MIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASICAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(16270..16962)
                     /gene="ygeA"
                     /locus_tag="OQQ48_001995"
     CDS             complement(16270..16962)
                     /gene="ygeA"
                     /locus_tag="OQQ48_001995"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HCS1952779.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(17091..18506)
                     /gene="araE"
                     /locus_tag="OQQ48_001996"
                     /pseudo
     CDS             complement(17091..18506)
                     /gene="araE"
                     /locus_tag="OQQ48_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
     gene            complement(18821..19582)
                     /gene="kduD"
                     /locus_tag="OQQ48_001997"
     CDS             complement(18821..19582)
                     /gene="kduD"
                     /locus_tag="OQQ48_001997"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HCS1952780.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPIVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(19612..20448)
                     /gene="kduI"
                     /locus_tag="OQQ48_001998"
     CDS             complement(19612..20448)
                     /gene="kduI"
                     /locus_tag="OQQ48_001998"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HCS1952781.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLHNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(20735..21916)
                     /locus_tag="OQQ48_001999"
     CDS             complement(20735..21916)
                     /locus_tag="OQQ48_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HCS1952782.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PCTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            22171..>22564
                     /locus_tag="OQQ48_002000"
     CDS             22171..>22564
                     /locus_tag="OQQ48_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCS1952783.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVS"
BASE COUNT         5789 a         6041 c         5434 g         5300 t
ORIGIN      
        1 caatttccag cccttcgccg gtacagatgc catgacaata acccgccctc tggagatgct
       61 tcgcgacggt aaatacccgc agccagattt catcttccag cgccagctta cgggtgctcg
      121 ttaaactttc acgctgtaac gcaggcagaa agcgtgcagc ccccagcaac aatacgctac
      181 tgatcgccat agcaatcaac acttccagca gagaaaaacc ttgctctttt acaggcatcc
      241 ttctgtttct ccttgctgac aaagccggag tcttccccac ggcgaaacca ccagccacca
      301 ctcgcccgtt gagtttttga agcgaatatg cccggcccat gcggtattgc gcaggccaaa
      361 gaaagcaagc gaaggtgtca ggtcgctcat ttcgacttcg ggccagcgtg gcacaaagac
      421 caatggtgaa ctgccatgac aggtattggc cccagcagcg gaactcacaa ggcaccataa
      481 cgtcccctcc ctgataacgc tgatactgtg gtcgcggtta tgccagttgg catcttcacg
      541 taaatagagc aaatagtccc gcgcctggct ggcggtttgc catagccgtt gcgactgctg
      601 ccagtattgc cagccataga gtccacttgc gcttagcatg accaaaatca gcatcgcgac
      661 cagcgtttca atcagcgtat aaccacgttg tgttttcatg ccggcagtat ggagcgagga
      721 gaaaaaaaga cgagggccag tttctatttc ttcggcgcat cttccggact atttacgccg
      781 ttgcaggacg ttgcaaaatt tcgggaaggt gtctcgaaga atttaatgga gggtaaaaaa
      841 aaccgacgca cactagcgtc ggctctggca ggatgtttcg taattagata gccaccggcg
      901 ctttaatgcc cggatgcgga gcgtagcctt caatctcaaa atcttcgaaa cggtagtcga
      961 agatggattc gggtttacgt ttgataatca acttcggcag cggacgcggt tcgcggctta
     1021 attgcagatg agtttgatcc atatgattgc tgtacaggtg cgtgtcgccc cccgtccaga
     1081 caaaatcgcc cacttccaga tcacactgct gcgccatcat atgcaccagt aacgcgtagc
     1141 tggcaatgtt aaacggcagg ccgaggaaaa cgtcacagga gcgctgataa agctggcaag
     1201 agagtttgcc gtctgccaca tagaactgga agaacgcatg gcacggtgcc agcgccattt
     1261 tatccagttc gcctacgttc cacgctgaaa caataatgcg gcgcgaatcc gggtcgtttt
     1321 tcagctggtt cagtaccgta gtgatctggt caatatgacg accatccggc gttggccagg
     1381 cgcgccactg tttaccatac actggcccga ggtcgccgtt ttcatcggcc cattcgtccc
     1441 agatggtgac attgttttcg tgtagataag caatgttagt gtcgccctgc agaaaccaca
     1501 gcagttcatg gatgatggaa cgcaggtggc aacgtttagt tgtcaccagc gggaatccat
     1561 cctgcaggtt aaaacgcatc tgatgaccaa aaatggaaag cgttccggtt ccggtacggt
     1621 cgtttttctg tgtgccttcg tcgagcactt tttgcatcag ttctaaatac tgtttcatgg
     1681 ttcctcagga aacgtgttgc tgtgggctgc gacgatatgc ccagaccatc atgatcacac
     1741 ccgcgacaat catcgggatg gaaagaattt gtcccatgct gatgtactgc acccaggcac
     1801 cagtaaactg cgcgtcgggc tggcggaaaa actcaacaat gatgcgaaac gcgccgtaac
     1861 caatcaggaa caaacctgag acagctccca ttgggcgcgg tttacgaata tacaggttga
     1921 ggataataaa cagcactaca ccttccagca acagctcgta aagctgtgat gggtggcgcg
     1981 gcagcacacc gtaagtatcg aaaatggatt gccactgcgg gttggtttgc agcagcaaaa
     2041 tatcttctgt acgggagcca gggaacagca tggcaaacgg gaagttcggg tcaacgcggc
     2101 cccacaattc accgttaata aagttgccca gacgcccggc accaagacca aacggaatga
     2161 gtggtgcgat aaaatcagag acctggaaga aggaacgttt agtacggcgg gcgaagataa
     2221 tcatcaccac gataacgcca atcaggccgc cgtggaagga catgccgccg tcccagacac
     2281 ggaacagata cagcggatcg gccataaact gcgggaagtt gtagaacaga acataaccaa
     2341 tacgtccacc gaggaagacg ccgaggaagc ccgcatagag taagttttca acttcatttt
     2401 tggtccagcc actgcccgga cgattcgccc gacgtgttgc cagccacatt gcaaaaatga
     2461 aacccaccag atacatcagg ccgtaccagt gaagcgccac gggtcctatt gagaaaatga
     2521 ccggatcaaa ctccggaaaa tgcagatagc tactggtcat ctgtcaccac aagttcttgt
     2581 tatttcgctg aaagagaaca gcgattgaaa tgcgcgccgc aggtttcagg cgctccaaag
     2641 gtgcgaataa tagcacaagg ggacctggct ggttgccgga taccgttaaa agatatgtat
     2701 atgatccgcg ctataaccct ccgcgaatca gcccgcccat gccgcgacgc tccataaagg
     2761 ctgcaacctg atgacgaact tcggtcgcca gttgcgcttc cagactacgc tgcgcaagat
     2821 tttctgcttc ggcaaaatca atgcgccgca gcaggtattt tacccgcgct acagaacgtc
     2881 cgttcataga cagatggcga tacccaagcc caatgaggat tgccacgcac atgggatcgc
     2941 ccgccatttc accgcacaaa cggagatcga taccatgtat ttccgcttcc cgggcgatca
     3001 ttgccagagc tcgtaacatt gcaggatgaa ggctgtcata aatgttcgcc acccgggtat
     3061 tgttgcgatc aacggccaga atgtattgag tcagatcgtt ggtgccaaca gagatgaaat
     3121 cgacccgctt tgccagatgc ggcagcataa ataccattga cggtacttcc agcatgatgc
     3181 cgatacgtgg tttgggaatt tcgtaaccga tcatctcctc gacttcacgt ccggcacgtt
     3241 caatcaggcg gcgtgcttcg tcaacttcat cgaggctggt gaccatcggc aacagaatat
     3301 tcaggttgcc cgtagcggca ttagcacgca gcatcgcccg cacctggatc aagaagatct
     3361 ccggctgatc gagcgtaatg cgaatcccac gccaacccag gcatggattc tcttcgctga
     3421 tcggcatgta aggcagctgc ttatctgctc cgacatccag cgtacgcaag gtgacgggtt
     3481 tatcattgaa catttgcagc atcccctgat actgcgccac ctgttcttct tccgacggga
     3541 aaccactttg cagcatgaat gggatttcag tgcgataaag accgatgcca tcaatacggc
     3601 tgcccagttt ttcttcatgt tccgggctta aaccagcatt gagcatgact tttatacgct
     3661 caccgctttt taactgcgcg ggtaaattga cgtcatcttc cgccagacgg ctaagctcaa
     3721 tctcttcact aattagccgc tgatattctt gcagcagtat cggctccgga tcgaccagca
     3781 attcaccgcg atagccatca acgatcagcg tcctacgatg cagcaccgaa ggctgaatat
     3841 ccgcgcccat cacggtaggg agccccagcg cacgtaccat gatcgcagca tgggagttgg
     3901 ctgcgccatc tcgcacgaca acaccgacta agcgatcctg gggcagctca gcaagagttg
     3961 tcgctgacag ttcatctgcc accagaatga aacgttccgg ccaggcgttc ggcccttgat
     4021 tagcgtcatc aagatgaaac agcaatcgct gacccagcgc acgtaaatcg ccagcccgct
     4081 ctttgagata gttatcgctt agcgcggcaa actgttcggc aaatttttca atgaccgttt
     4141 ttaccgccca ctctgccacc gagcctttat caacctcggc aaacaattcg cgacgcagcc
     4201 gggtgtccga aagcaggtgc gagtaaagat cgaaaatagc cgccgtttct ttttgtgctc
     4261 cggcggcaaa gcgtttgctg tagcggcgaa actcgtttgc cgcttcttcc agcgccccgg
     4321 tcaatcgttc gcgttccaga gccggatcca gcgttgatgc ctgatacacc tgttccatta
     4381 aaggtaacgt ggcatcctgc cagccttcgg caatcgccac accaggtgct gccggtaatg
     4441 cgcggattcg cgtctggcga tattgcccaa acaaggcagt caactgcgac tgagaaagaa
     4501 tagctgccat ctgggtggca agcgtcacca ggaaggattc ttcactttcg tcatactggc
     4561 gcaactctcg ttgctgtacc accagtacac caagcaactg gcgacgttga ataattggta
     4621 cgcctaaaaa cgcgcggaaa cgttcttctt ttacggaggg gatgtatttg aagctggggt
     4681 gcttttgcgc atctgcaagg tttatcggtt ccgccagcct gccaaccagg ccgacgatcc
     4741 cttcatcaaa cgcgagcgtt acagtgcgac cgcgtggttt tttcagcccc cgggtcgcca
     4801 tcaggtagta acaacgtcga tcatgatcgg ccaggtagac cgaacagacc tcggtatcca
     4861 tcgcaagaca gatgtcggta accagaatat ttaacgcctc attcaggcgt ggtgcgctgg
     4921 ctaccttttc gactatttcg cgcaggcgag tgagcataat tggcgtgact taacctcttt
     4981 tacgtcgata agcagatgtg ttttgtggtt ttggcgtatt ttcctgcagt gacatcacca
     5041 cactcgcgaa ctctttcatt accctacggt agacatcacg tttaaatgac accacctgtc
     5101 tgaccggata ccagtaactt acccatcgcc agccgtcaaa ctctggtgta ctgctggttt
     5161 gcatattgat ttctgcatcg ccgctcacca gctgcaagag aaaccatttt tgtttttggc
     5221 cgatacaaac cggcttcgtg tcccaacgca ccaaacgttt cggtaatttg tagcgcaacc
     5281 agttacgcgt tgaagcaagg attcgaacgt ctttgcggct taatcctact tcttcaaaca
     5341 attcacggta catcgcctgc tctgcggatt ctccggggtt gattccgcct tgcggaaatt
     5401 gccaggagtg ctgaccaaat cgccgggccc acattacctg cccctggcga ttacaaatca
     5461 cgatacctac gtttgggcgg tagccatcgt catcaatcac cggactacct caaaataaag
     5521 ctttatatac gaatgattgt ttcatactcc aggaagacgg taaaccactc tctgcagggc
     5581 attacacact aataacaatt gaataactca cagttatgtg cagagttata aacagaggaa
     5641 ggggtggata gccgttttta ttcacctttt ctgtggatag agttgtgaag aactacggaa
     5701 ttactacggg aaaacccgga gcattctgaa taagcccgat ataggcattt taaaatattc
     5761 attataagtc aatgcattac atgtaatttg agtaatgaaa aagagtaatt cgtgacccag
     5821 gtcacacctc tcatttacgg gttggcgaaa gatcaaccaa tgccgtattt atccacagaa
     5881 tgtgccacta agttaagcac tgaaccacta aaaactggag tttcgtcgca cgtcaaggct
     5941 gtaaatggaa acagtagtgg aggtttttca cagttatccc agctttctgt ggataacatg
     6001 gtgtaagatc ctgtttattt tcagtgacca gatttggaaa acctattgca gtgctgcgca
     6061 actcgattat cggcagccta aaaagcaata taaatcagag aattgaacaa cacatgtgga
     6121 aaaagttaca ctgcgaatat tcggtacata attgctgttt gttttttaac caaggtatca
     6181 tgacatgtcc caacctcgcc cactgctctc tcctcccgaa actgaagaac agttgttagc
     6241 gcaagcacag caactttctg gttatacatt gggagaactg gcggcacttg ccgggctggt
     6301 tacgccagag aatttaaaac gcgataaggg ctggattggc gtgttactgg agatctggct
     6361 aggtgccagc gcagggagta aacctgagca agattttgct gctctgggcg tggaacttaa
     6421 aactatccct gtggatagtc ttggtcgtcc gctggaaaca acattcgttt gtgttgcccc
     6481 gttaacgggt aatagcgggg tgacctggga aaccagccac gtgcgccaca agctcaaacg
     6541 cgtactgtgg ataccggttg aaggcgagcg cagcatcccg ctggcgcagc gtcgcgtagg
     6601 atccccgttg ctgtggaccc cgaatgaaga ggaagaccga cagctacgcg aagactggga
     6661 agaattaatg gatatgattg ttctcggtca ggttgagcgg atcaccgctc gtcacgggga
     6721 atatttacag ataagaccga aagcagcgaa tgcgaaagcg cttaccgaag ccattggtgc
     6781 ccggggcgaa cggattctga cgctgccacg cggcttttat ttgaagaaga atttcaccag
     6841 tgcactactg gcccgtcatt ttctgatcca gtagccatcg ctttgacctg ccgctttccg
     6901 ggcatataat taccgcttca tttttttggc agggcttttt agatgttatt tgcatggata
     6961 accgatccta acgcctggct tgcgctcggt acgctgacgc tgctggagat cgttcttggg
     7021 atcgacaata ttattttcct ttctctggtg gtggcaaagc ttcccacagc acaacgtgct
     7081 catgcgcgcc gtctggggtt ggcgggagcc atggttatgc gtctggcgct gctggcatca
     7141 atcgcctggg ttacgcgcct gacaaatccg ctttttacaa tattcagtca ggaaatttct
     7201 gcccgtgatt tgattctgct tctgggtggc ttgttcctta tctggaaagc cagcaaggaa
     7261 atccacgaat ccattgaagg tgaagaagaa gggctgaaaa cacgcgtttc atcattcctc
     7321 ggcgctatcg tgcagattat gctgctggat attatcttta gcctcgactc ggtgattacg
     7381 gctgtgggtc tgtcagatca cctgtttatt atgatggcag ccgtggtgat tgccgtaggc
     7441 gtgatgatgt tcgccgcgcg ctcgattggt gattttgtcg aacgccatcc ttcggtaaaa
     7501 atgctggcgc tctctttcct gattctggtg ggctttaccc tgattctgga aagtttcgac
     7561 atccacgtac cgaaaggtta catctacttc gcgatgttct tctctattgc ggttgaaagc
     7621 ctcaacttga ttcgcaacaa aaagaatccg ctctgatact ccgtacgctc tcctgcggga
     7681 gggcgtactc actcctttta ccctcacagt ttaagatttt cctgctttca agatatataa
     7741 cgtcgtttta taaacagact attatcatag gtaggccaaa tgaggataac cggatgaaaa
     7801 aatgggcagt aataatttct gcagtcggac tggcgtttgc tgtttccggg tgttccagtg
     7861 attacgtcat ggcgaccaaa gatggccgta tgattttgac cgatggaaaa cctgaaattg
     7921 atgatgatac cgggctggtg agttatcacg atcagcaagg taacgcgatg caaattaacc
     7981 gtgatgatgt ttcgcaaatt attgaacgtt aacaaataag gtcagcatcc ggctggcctt
     8041 aagatttttc tctttccctt ttcccttccc tctgccattt ttatattcct tatgtcgtga
     8101 ttataaaaag gaaacggcta tgcaatatca ccgtataccc cacagttcgc tggaagtcag
     8161 cacgctgggg cttggcacga tgacgtttgg tgaacagaac agcgaagccg acgcccacgc
     8221 acaactcgac tatgccgtcg ctcagggcat taaccttatc gacgttgccg aaatgtaccc
     8281 agtacctccg cgccccgaaa cgcaagggtt aaccgaaacc tacgtcggca actggctggc
     8341 gaaacatggc agccgcgaaa agttaattat cgcctccaaa gtgagcggac cgtcgcgcaa
     8401 taatgacaag ggcatccgcc cggatcaggc gctggatcgg aagaatatcc gcgaagcgct
     8461 gcatgacagc ctcaagcgcc tacagactga ttacctcgat ctttatcagg tgcactggcc
     8521 gcagcgcccg accaactgct tcggcaaact cggttatagc tggacggatt ctgcgcctgc
     8581 ggtttcgctg ctggatacgc tggacgcact ggcagagtac caacgcgcgg gaaaaattcg
     8641 ttatatcggc gtgtcgaacg aaactgcatt tggcgtaatg cgctacctgc atctggcgga
     8701 caaacacgat ctgccgagta ttgtcaccat tcagaaccct tacagtctgt taaaccgcag
     8761 ttttgaagta ggtctggcag aagtcagcca gtatgaaggg gtcgaactgc tggcctattc
     8821 atgcctgggt ttcggcacgc tgaccgggaa atatctcaat ggtgcaaaaa ccgctggcgc
     8881 acgtaatacg ctctttagtc ggttcacccg ctatagcggt gagcaaacgc aaaaagccgt
     8941 cgcggcgtat gttgatatcg ccagacgtca tggcctggac cctgctcaga tggcgctcgc
     9001 gtttgtacgc cgtcaaccgt ttgttgccag cactctgctg ggcgcaacca cgatggatca
     9061 gctgaaaact aacatcgaaa gtttgcatct ggagttaagc gaagacgtat tagctgaaat
     9121 tgaagcggtg catcaggttt atacttatcc ggcaccataa gattaacgcc ggttaaaacc
     9181 ggcgttaaat attaatgacg gcgctgccag atccacagcg ccgttattgc cagcgcaaac
     9241 agcgcaccga agccaatgcc aatgggcacg accgggatgc ctaccattac cgccagcgag
     9301 taaatgccca gcatcaacaa catggcgctg ttttcgccaa ggttttgtac tgcaatcgca
     9361 ttccccgccc cgacgctttt tttaccccgc tcctgtagca acgcattgag cggaacgaca
     9421 aaaaagcccc ccatcacgcc aatcagcatc aacaaggcat aggctggcag cagctcgtgt
     9481 tgcagggaaa aaatcagtac caccacgcca atcaaaatcc cggctggcat acagcgtgac
     9541 acggtttcca gcgtaactaa cttcgctgcc gcacctgcgc caaccacgat accaatcgct
     9601 accatcgcgt tgagataggt gggcgtagag ttatcggtaa tgcccagcgc caccggtacc
     9661 cacagcacca acaggaaacg cagcgtgaca cccgctcccc agaataaact ggtgcccacc
     9721 agcgaaaaac gcgtttcacc attgcgccat agcgaggtgc aggcattcag gaaactgcgg
     9781 gtcatgttga tgagattcca agactgcccc ggacgcgccg ccgccagttt gggaatgtag
     9841 atattggcaa cgaccgcacc accgtaggcc agtgcgcatg cggccagggc gacgaggaca
     9901 tgccagtcag ccagcacacc accggctacg gaaccgagca aaatcgccgc tatggtagaa
     9961 gcttccatta aaccgttagc tttcactaac ttactacccg tggttaattc gccgagaata
    10021 ccgtatttcg ccggtgaata ggctgcagca ccaaccccca ccagcgtata gccgagaaac
    10081 ggattgatac caaagcagat actggctgcg cccagcagct tcaggccgtt ggcaaacatc
    10141 atcacccggc ctttggcgaa gctatccgcc acctgcccga caaacggcgc aaaaagaatg
    10201 taagcaccta caaacaccat ttgcaggatg ggctggctcc actccggata gaactgcgct
    10261 ttcagtaacg ccagagtggc aaacagtagg gcattatcgc caaacgcaga gagaaactgc
    10321 gccacgataa ccgctttcat ccccttcgac cacaacgaag tgttagtgtg cactgactca
    10381 ctcatcgtgt tgttccgctt cgtctaccca gcttttcaac gtgacaaagt caggtttgcc
    10441 gctgccaagt aatggcatct gtttcagata gcgaatatcg cgcggtacag caagctccgg
    10501 cacgccgtgc tcgcgggcat actgttgcaa cttatcgagc gtcagttcgt tatctgtggt
    10561 gaaaagcacc agtgcctcgc ctttgctggc atcgctctta atcgcagtgg catggacttt
    10621 atctggcgaa acaccaagtg ccagttgttc caccatttcc agcgacacca tttcgcctgc
    10681 aattttggca aagcgttttg cgcggccctg aatctgcaca aagccctgct cgtcaaaacg
    10741 cacaatatcg ccagtgtcat accagccgcg ctccatttcg ccgcgaacat tctcggcggt
    10801 gggcacttcc agtacacctg gcttctccac ccgcagatag ccgttcatta tgttcggccc
    10861 tttcagttgc aggcgtccgc cctcttcgat accaggaacc gacaacagac gcgcatccat
    10921 tcctggtaga atacgcccta ccgtaccggg tttcgccgcc atcggtacgt tgatagaaac
    10981 gacaggcgcg cactcggtta caccatagcc ttcaaggatg cgcaggccaa atttatcctg
    11041 ccaaagctgt ttggtacttt cttgtagttt ttctgcgcct gccaccacat agcgtagacg
    11101 atagaagtca tacgggttgg cgaaacgcgc gtagtgaccg aggaaagtcg aggtaccgaa
    11161 caacacggtg caactgcggt catacaccaa ctccggcaca atgcggtaat gcagcgggct
    11221 tggataaagg aacacttctg cacctgtaag cagtggcgta aacaggccta ccgtcagccc
    11281 aaaggagtga aacagcggta atgccgacat aaagcgatcg ttggtggtga agtcggcaat
    11341 cgttttaatc tgctcgacgt tcgccagaat gcttttatgg ctatggacga cgccttttgg
    11401 atggccttca gaaccggagg taaagaggat cagcgcctct tcttccggct gctgttttac
    11461 ctgcgccagg cgcggcatca gcaaatgagc gaagatccat actttgtcgg cagtggtgac
    11521 atctgctttt aaatcttcca gatagaccca gcgcacctga gtaagttgct ccggcagatg
    11581 ccagagtttg cctttatcga gaaactggcg ggaagtgaag atggttttga tttcagccgc
    11641 cgtaatagca ctggtcagcc cttttacccc agcagtgtag ttcatcattg cgggaatgcg
    11701 gcgacgcgcg atggccccaa aaatcactgc cgcactgatg cctgcattgg gcagcattaa
    11761 gccgatgcgt tcgccttcaa cactgtattt ttcaaggatg cgtccaacaa acagcgtttt
    11821 cgtaagcaat ttgcgatagg agtctggggt aaagttgacg tcttcgacac atttcttccc
    11881 ggctccgaag cggtacattg cactcagtaa agattcgtac agagtttcac gcgggcgcac
    11941 cgccatacgc gcttccatca ttatttgatg cagcatttcg ccagcgattt tgcgacggtc
    12001 acgggcacgc gacgcatccg gcatcgccac ctgcgttgga ggcaaaatat gcagagtaat
    12061 ttgcgggaac aagcgacgtt taaccagacc tttcaggcgg ctgaagtgcg taagttccgc
    12121 cccttcaata cgcacaggaa taaccgttgc accagacttc gccgcgacaa aacccgcgcc
    12181 atcgtagatt ttcatcagcg agcctgtagt ggtgatgcgt ccttcaggga aaatcaccac
    12241 tggtcggccc tgttccacca gacgtaccag atgtttaata gccataggtt gcgtcgggtc
    12301 gagaggaaca aagtcgataa atgatttcag ccaacgcata taccactgtt ggcttattga
    12361 ggtgtaaacg gcaaacactg gacgcacagg taaaaacagt ccaagcaaaa tgccatcaat
    12421 aaaagagacg tgattaggcg taattagaac gcgctcgccc ttcagtgcct gggtgtcacc
    12481 cgtaacgcga acgcgataca aaacacggca caaatttcga aaaaagctaa aaagcattcc
    12541 aactcccttt gctctgattc agtaaaagcg aatggaggga gattacacga gataaagaac
    12601 gcgagcgaca gtaaattagg tgcgaaaaaa aacctgcgca tccgcgcagg ttggtgcaag
    12661 agacagggta cgaagagcgt accgaataat ctcaccaatc aatacctctg ggatcaccac
    12721 tttagcaacc tgaagccaaa cgccaccagc ggtcaatcgc aacagcgttt cgcaaagtgt
    12781 aaccaaaggt ttgcaatctc tttttttgtc ttacttattt gcgtttggct cacattccca
    12841 cgatgaaaac acgccactcc ttgaaccaac gggcgttttc cgtaacactg aaagaatgta
    12901 agcgtttacc cactaaggta ttttcatggc gaccataaag gatgtagccc gactggcagg
    12961 cgtttcagtc gccaccgttt cccgcgtcat taataattca cccaaagcca gcgaagcttc
    13021 ccggctggct gtgcatagtg caatggagtc tcttagctat cacccgaacg ccaacgcccg
    13081 tgcgctggcg cagcagacca ctgaaacggt cggtctgatc gttggtgatg tttccgatcc
    13141 gtttttcggt gcaatggtga aagcggtcga acaggtggct tatcacaccg gtaatttctt
    13201 attgattggc aacggttacc acaacgaaca aaaagagcgt caggctattg agcaactgat
    13261 ccgccatcgc tgtgctgcgt tggtcgtcca tgccaaaatg atcccggatg ccgatttagc
    13321 ctcattaatg aaacaaatgc ccggtatggt gctgatcaac cgtatcctgc ctggctttga
    13381 aaaccgttgt attgctcttg acgatcgtta cggtgcctgg ctggcaacgc gtcatttaat
    13441 tcagcaaggt catacccgca ttggttatct gtgctctaac cactctattt ctgacgccga
    13501 agatcgtctg caagggtatt acgatgccct tgctgaaagt ggtattgcgg ccaatgaccg
    13561 gctggtgaca tttggcgaac cagacgaaag cggcggcgaa caggcaatga ccgagctttt
    13621 gggacgagga agaaatttca ctgcggtagc ctgttataac gattcaatgg cggcgggtgc
    13681 gatgggcgtt ctcaatgata atggtattga tgtaccgggt gagatttcgt taattggctt
    13741 tgatgatgtg ctggtgtcac gctatgtgcg tccgcgcctg accaccgtgc gttacccaat
    13801 cgtgacgatg gcgacccagg ctgccgaact ggctttggcg ctggcggata atcgccctct
    13861 cccggaaatc actaatgtct ttagtccgac gctggtacgt cgtcattcag tgtcaactcc
    13921 gtcgctggag gcaagtcatc atgcaaccag cgactaaccg cagttaaagc aattccagcg
    13981 ccagtaattc ttcgatggtc tggcgacggc gaatcaaccg cgcctgacca ttatcaaaca
    14041 gaacttctgg taacagcgaa cggctattgt agttggatga cattgatgcg ccatatgccc
    14101 ctgtatcatg cagtaccaga taatcacctg ccttcacttc cggcaaggcg cgggtttcaa
    14161 catttccccc ttcctgctgg gtaaagacat cgcccgattc acataacggt ccggcgacga
    14221 cggtttccac cgttggcgcg tgttccagag aacgaccatc agctgccagg gcactgatat
    14281 ggtggtaact accgtacatt gccgggcgca tcagatcgtt gaacccggca tcaaccagca
    14341 caaagtggcg gctccccatt tgtttgacgc tccgcacctg agtaattaat acgccagact
    14401 gcgctaccag gaagcgaccc ggttcaattt ccagtttcac agggtggccc aaatggcggg
    14461 cgatttgctc acgcgcggca ttccacagac cataataatg ttcggtatca accgcctctt
    14521 caccctgttg ataaggaaca gaaagcccac cgcccgcaga aatagcctgt aaatcctgac
    14581 cgaattcgat gacctgacgc accatagcac cacacacctg ttccagatgg gcataatcaa
    14641 cgccagaacc aatgtgcatg tgaatgccga ccagctgcag atgatgacgt tgtatcacgt
    14701 ccagtgcggc gggcagatcg gtgtaccaga taccgtgctt gctgttttcg ccaccggtat
    14761 tggttttttg gctatgtccg tgaccaaacc ccggattaac gcgcagccat acccgatgcc
    14821 ctggcgaaac ctggcccagt tggtcgagca tatcaacaga acccgcattc accggaattt
    14881 gcaattcact gacgcgttca agcgtcgcct gatcgataac atctgccgta aaaacaatat
    14941 catcggggtg cgtttgcgga ttgtaacccg ccgccaacgc acgctctatt tcgcctaacg
    15001 agacggaatc cactttcacg ccctgctcac gcattaagcg caaaatatga atattggaac
    15061 aggctttctg tgcaaagcgc accacatcaa actgtttcag cgctgcaatc tgccgacgaa
    15121 taatttgcgc atcgtagacc cacaccgggc agccaaattc agcgggcaaa cgcagcagat
    15181 tttcggcggt gagatcggta tcggtgctga acagtgaatg tggcataaca aactccagat
    15241 aagtgctttt ttatgattac gccacatcat aaaaagaata aaaaatatcg atttatgtcg
    15301 agtctatgca aaaatgatat ggattaccgg attgcgagag agcgctaatg gccgccgtta
    15361 acttacgtca tattgaaatt tttcatgcgg taatgaccgc cggaagcctg actgaggcgg
    15421 cacacctgct acacacctca cagccaaccg tcagccgcga acttgcgcgc tttgagaaga
    15481 tgatcgggct gaaattgttt gagcgcgtac gtgggcgatt acatcctacc gtgcaaggac
    15541 tgcgtctgtt tgaagaagtg caacgatcct ggtacggact ggatcgaatt gtcagcgctg
    15601 cagaaagtct gcgcgagttt cgccagggag aactgtctat tgcctgcctg ccggtctttt
    15661 cgcaatcttt tttaccgcag ctcctgcaac cctttctggc acgttatccc gatgtcagct
    15721 taaatatcgt gccccaggaa tcaccgctac ttgaagagtg gctctcggcc cagcgtcatg
    15781 atttaggact cactgagaca ctccatacgc ctgcgggaac agaacgtacc gaattactct
    15841 ctttagatga agtgtgtgtg ttacctccgg gtcatccgct ggcggtaaaa aaggtattaa
    15901 cgccggatga ttttcagggt gagaactaca tcagcctttc ccgtactgac agctatcgcc
    15961 agttgctgga tcagctattt actgaacatc aggttaaacg acgcatgatc gtagaaaccc
    16021 acagcgccgc gtcaatctgc gcaatggtac gggcgggggt aggtgtttcg gtggttaacc
    16081 cgctcaccgc actggattat gcggcaagcg gtttagtggt gcggcggttc agtattgcgg
    16141 ttccgttcac agtcagcctg atccgccccc tgcaccgccc gtcatcagcg ctggtgcagg
    16201 catttagtga gcatttacaa gcggggttac cgaaactggt cacttctctt gacgctattt
    16261 tgtcgtcagc tacgacagca taaaagcgac agcatcctcg gcatggatcg ccgcagtatc
    16321 aaacacaggc aaaacactgc gctcttctgg caccagtaaa ccaatttctg tgcagccaaa
    16381 aatgacgccc tgtgcgccct gttctgcaag gcgagcaatc acttgcgcat aataagcgcg
    16441 tgacgcttcg gtaaattgcc ccagacacag ttcttcaaaa ataatctgat taattttcgc
    16501 ccgttcatcc gcttcaggaa taagacaatt gatggaaaat tgttccgtca gccgcccgcg
    16561 ataaaaatcc tgttccatgg tgtaacgcgt acccagcagc gccacacgag tcattccggc
    16621 cccggtaatt gcacgtccgg tggcatccgc aatgtgtaag aaaggcagag tgcaacgtga
    16681 ctcaatggca tccgccactt tatgcatcgt attggtacat aacacaatac cttctgcgcc
    16741 cgcccgctgt aagccaagcg ccgcctcagc cagaatgtcc ccggttttat cccattcccc
    16801 gcgacgctgg cactcttcta tttcatgaaa atcgacgcta tgtagcagca cttgcgcaga
    16861 gtgaagccca ccaagccgct gtttaatgcc ttcatttatc aaacgatagt aaggaatggt
    16921 ggattcccag ctcattcctc ccagcaaacc aattgttttc attcctctct cctgatgtgt
    16981 gttaccgcaa caaggctgaa taaggaacga gacaaacgcc tcaacggcca agtgcccaat
    17041 ctctattaac gaaaaaaggg ccggatgtac agcacatccg gcccgtgaaa tcagatgccg
    17101 atatttctca acttctcgcc tgccatcagt ttgcgttcga tatgttccag cgtgacattt
    17161 ttggtttccg gaatgagcca gaaagtgatg cccacaaacg caatgttcag cgcagtgtag
    17221 agccagaacg taccggcagc gccaatgctg tcgagcagcg tcaggaaggt cgccgataat
    17281 catattcgac acccagttcg tggtggtcga acaggtaata ccgaaatcgc ggcatttcag
    17341 cggctgaatt tcagagcaca ggatccacac cactggcgcg gcgctcatcg cataaccggc
    17401 aatacacatc atcgtcatgc caacagagag ccaggacaat ccactggaag ccgtaccgtt
    17461 atcaaactgc atcaggcaat agcccagcac cagagtgcct aacgccatca cgctgaaacc
    17521 aattttcaga gccggtttac ccctgcttta tctaccgtaa acaccgcaat aaaggtggcg
    17581 aacataaagg tcagccctac gaccagagtc gcaatcatct gttgttctgt ggtcgtaaag
    17641 cccgccattt tgaagatacg cggcgcgtag tacatgatga tgttcatacc ggtaaactgc
    17701 tgcatcgcct gcaacaacat accgagaaac acagcacgac ggacgttacg gttgatctta
    17761 aacagtgccc aaccgccctg ttttaacttc aggctttcac gaatttcgtt gagttcttct
    17821 cgcgcttttt ccgacgtatc gcgcagcata cgcaatactt cttccgcctc aatatgacgc
    17881 cccttttccg ccaaccagcg cgggctattt ggcaggaaga ccaccagaat aatcagcaga
    17941 actgctggta aagcaagaac ccccaacatt gcgcgccagt taccgctata actgaacgct
    18001 gtatcggata aaaacgccag cacgatgccg agtgtgacca tcaactggta catactgatc
    18061 atcttaccgc gaacgttttc acttgccatt tcagaaagat acagaggagc ggtgtaagac
    18121 gcgatcccga cagcaatgcc cagccccaca cgagcggcga ttaacatctc tacgctggtc
    18181 gcaaaagcgg accctataga accgagtaca aacaggatgg cccccgccat caggctgtat
    18241 ttacgcccca ggcggaacga cagccaacca ttaaacagcg caccaattgc tgcgccgagc
    18301 atcatgctac taaccaccca ttcctgcaaa tgactggtca gcacaaagtg atcggtaatg
    18361 aacggcaacg ctccggcgat tacgccgata tcaagaccaa ataacaatcc tgcgaccgca
    18421 gcagctaccg aaacaaacat attcatacgc cgcgtatccc gcaaagaacg tggcgttaaa
    18481 gcagattccg tattgatagt aaccattttt tcctgccagc agagagtaag acatagtgaa
    18541 aaaatacgtg aacaactcac gcaggtgtca ggtcggaaac agcataaata tggattaaat
    18601 tgctgcgaca tgtcgttatg tgatggatat tccaattttc aaattaagtt gaattattga
    18661 gattattatt aaccacctaa ttttacagca gataaaattc ataaagttca ttaattgata
    18721 attaatatgg attatttcat aaccatgata tggattatga tgatctacag gtataaaaaa
    18781 ccctgccatg cggcagggtc ataaaagtaa gaagaatgaa ttaacgcgcc agccaaccgc
    18841 catccacggc aatggtataa ccattcacat aatctgaagc gctggaggca aggaacacta
    18901 tcggccccat caggtcactc ggcagtcccc aacgaccagc tggaatgcgg tcgagaattt
    18961 ccgcgctacg ttgttcatct gcccgtagtt gttgagtatt gttggtcgcc atgtaacccg
    19021 gagctatcgc attaacatta atgttgtgtt tagcccattc gttcgccatc aatcgcgtca
    19081 cacccatcac gccgcttttt gatgcggtat aagaaggcac acggatcccg ccctggaagg
    19141 agagcattga cgcgatattg ataatcttgc cgccattgcc ttgcgcgata aagtgtttcg
    19201 ccgctgcctg agacatgaag aatacgctct tgatattcag gttcatgaca tcgtcccagt
    19261 ctttttcgct gaactcgaga gcgtcttcgc ggcgaatcaa tccggcgtta ttcaccagga
    19321 tatcaatatg accaaattca gctaccgcgc gatccagcag tccaggaatg ccatcaatct
    19381 ttcgcagatc ggcggtcagg cttaaaaaac gacgccccag cgccgtgacc tgctcgatgg
    19441 tttcagtcgg ttcaacgatg ttaatgccaa caatgtcaca gcccgcttgc gccagcccca
    19501 acgccatccc ctggcccagc ccagtatcac aaccagtgac gaccgcaact ttaccttcga
    19561 gagaaaatgc acttaaaatc ataacaatac cttattcgtt tatgcccaca actagcgcaa
    19621 atctttaacg gccacatggt ccatatcatc aaagacctgg ttttcaccaa ccatgcccca
    19681 gataaaggta taagctttgg ttccgacgcc ggaatggatc gaccagctcg gggagatcac
    19741 cgcctgctcg ttatgcatca caatgtgacg cgtttcttgc ggttgcccca tcatgtggaa
    19801 aacgcaggcg tcatcatcca tattgaaata gaaataaact tccatccggc gctcgtgggt
    19861 gtgacacggc atggtgttcc acaagttacc tggagccagc tccgtcagcc ccatactcaa
    19921 ttggcaggtt tccagcacat ccgggacaaa atatttgtta atagtgcgac ggttactggt
    19981 gaggttatcg cctaacgtga ctggagatac ttcgtccggg gtgacttttt tggtgggata
    20041 cgtcgtatgc gcgggtgcgc aattgtaata aaactttgcc ggagtggcgg tatcaatact
    20101 ggcaaagaca acttcttttg cacctttacc aacatacagg gcgtcgcggt gaccgatttc
    20161 atagcattgg ccatcgacag taatcgtacc ggcaccgcca atattgataa cacctaactc
    20221 gcgacgttca aggaaatagc ttacgccgag ttgtttacca acttccccgc caacggaaac
    20281 cgttttagtt atcggcataa tgccgccaac aataattcgg tcaatgtggc tgtaaaccat
    20341 ggtgtactca tcggcgacaa ataccttttc aaccaaaaat tcattgtgca gcccttgggt
    20401 atccagcgtt tttgcgtgcg cactgtggat gctctgtctt acgtccacat caacctccga
    20461 taatcagtga agcgtgaaaa tagaaaacga tagtgaaaca ctgtttcgtt ttctctataa
    20521 agggatgata tgcccatggg ctagggtttt caattaaaaa taaaacaatg ttttattttt
    20581 aagttaagga ttaaaaaaag tgcagtgtcg atcacgaata tgtcatgaga atggaaaaat
    20641 gagggagccc agaaaatgta tgtcgacttg ccagccatca ttattcaaca gattaatgac
    20701 ggcagatatt tatcggtaag gaggaaatgc agagctactc gtcacgttca atggtcaatg
    20761 ccacgccctg gcccccgccg atacaaagcg ttgccagccc tttgcgggca ttacgtttca
    20821 ccatttcatg aaccagagaa accaggattc ggcaaccgga agcgcctatc gggtgaccga
    20881 gtgcgatcgc gccaccattg acattgaccc gacgctcatc ccactcaagc atcttgccaa
    20941 ccgaaagcgc ctgtgcagca aacgcttcat tagcctcgat aagatcgaca tcagccaact
    21001 gccagcctac acgctccagg caacggcggg tcgcatacac cggcgcaatt cccatcaatg
    21061 ccggatctac accaacgctg gcaaatgcgc ggatgcgggc cagcacgggt aaattcaacg
    21121 ctcgtgcttt ggcttcgctc atcatcatta ccgcagctgc gccatcgttt atggatgatg
    21181 cattccccgc ggtcacagaa ccgagactat caaatgaagg atttaaacgg gctaaacctt
    21241 ctgcactggc gtcagtgcat ggctgttcat cggtatcaac aaccaacgtc tgaccgttac
    21301 tttggatcat taccgggacg atctcatctt taaatcgtcc ggcgtcaatc gccgctcgcg
    21361 ctttttgttg cgagctaagt gcgtaagcat cctgcaactg acggctgatg ccatattcgc
    21421 gagccagatt ttcggcggtg acaccaatat gataatcatt gaaggcatcc cacaacccat
    21481 catgcaccag actgtcaacc aactggctat tgccaagctg tgcaccggtg cggctatcag
    21541 tcagaacatg tggtgcgcgg ctcatgtttt cctggccacc ggcgatgaca atatcagcct
    21601 cgccacactg tatcgcctga gtagccagat gcagtgcttt aagcccagaa ccgcaaacgt
    21661 cattaatagt gattgcagaa acgctattag gaagaccacc tttaatagcc gattgccttg
    21721 ccggattctg ccctgcccct gcagtcaaca cctgaccaag aattacttca tccaccgcat
    21781 atgcaggaac gccggtacgt tctattaacg ctttcacgac caaactacca agttccacgg
    21841 cggaatgacc cgctaacgca ccacgaaagc agccgatagg tgtccgtaac gccccgacaa
    21901 tcacaacgtc tttcatcaca acctcgcacc aattaacagt gcaatagtaa atgattgtta
    21961 attacagtta tcttaattgt ttaaaaaaag tgatttttat cacaaaagaa atatgcctga
    22021 gcagcagtca gagacataac tggcacgtaa ggtttgcaac cactaaccca ccaatagagg
    22081 ggtagatagg gcgttaatct cccatactta acctggttta tggtaaattg ccctccattt
    22141 tgtttaattt gtagatgata cgttcagata atgtctaata tttggtctaa agaagaaact
    22201 ctgtggagtt tcgcgctcta cggcacagcc gttggtgcag gcacgctctt ccttcctatt
    22261 cagttaggtt cggcaggggc tgtggtcctg tttattactg ctctggtcgc ctggccttta
    22321 acatattggc cacataaagc tttatgccag ttcatcctct catcgaaaac atcagcaggt
    22381 gaagggataa cgggcgcggt aacacactac tatggcaaga agattggtaa tctgattacc
    22441 acgctgtact tcatcgcctt ttttgtcgtc gtgttgatat atgcagtggc aattaccaac
    22501 tcacttacag aacagctggc aaagcatatg gttattgatc ttcgcatccg tatgttggtg
    22561 agtc
//
LOCUS       DAJVUJ010000058        22470 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.058, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000058 DAJVUJ010000000
VERSION     DAJVUJ010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22470
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.058"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            52..243
                     /locus_tag="OQQ48_002001"
     CDS             52..243
                     /locus_tag="OQQ48_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCS1952784.1"
                     /translation="MKYGKLGYIVPRPQKIRGRWLIDRRAVFVGPGETGIAPEIHTGD
                     DDALKEILTHVTEATKKQH"
     gene            212..1318
                     /locus_tag="OQQ48_002002"
     CDS             212..1318
                     /locus_tag="OQQ48_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HCS1952785.1"
                     /translation="MSPRPRKNSTDVAGLYEKFDRRTGRVYYQYKNPVTGKFHGLGTD
                     KGKAEKIASTANQRIAAAEAEYFMRKIDESPSATKRRGIRLKAWVDRYLKIQDTRLKN
                     GDIAATTHKEKTRMAAYLVSRLGNHPLKELEVRDFALILDEWLDKDMVSTARVNRGLW
                     VDIYKEAQHAGEVPPGWNPPEATRKPIPKVTRARLTMEDWQKIYNATPEKHFIRNAML
                     LAIVTGQRRDDICHMRFSDVWNEHLHITQGKTGMRLALPLTLRCDAIGITLKEVIDGC
                     RDRILSPYLIHSRHQKQPKPMSKDNLSDYFAKARDLAGIIPPAGKTPPTFHEQRSLSE
                     RLYRAQGIDTKTLLGHKVQATTDRYNDTRGQEWV"
     gene            1498..2292
                     /gene="potC"
                     /locus_tag="OQQ48_002003"
     CDS             1498..2292
                     /gene="potC"
                     /locus_tag="OQQ48_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HCS1952786.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMISPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            2289..3335
                     /gene="potD"
                     /locus_tag="OQQ48_002004"
     CDS             2289..3335
                     /gene="potD"
                     /locus_tag="OQQ48_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HCS1952787.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETCIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(3491..4312)
                     /gene="cobB"
                     /locus_tag="OQQ48_002005"
     CDS             complement(3491..4312)
                     /gene="cobB"
                     /locus_tag="OQQ48_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCS1952788.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     KIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(4328..5239)
                     /gene="nagK"
                     /locus_tag="OQQ48_002006"
     CDS             complement(4328..5239)
                     /gene="nagK"
                     /locus_tag="OQQ48_002006"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HCS1952789.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCRGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQQLQAPEIIALYNQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(5268..6512)
                     /gene="lolE"
                     /locus_tag="OQQ48_002007"
     CDS             complement(5268..6512)
                     /gene="lolE"
                     /locus_tag="OQQ48_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HCS1952790.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDKVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHVAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEMTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     LIIGVVVSLQLTPIIERIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(6512..7213)
                     /gene="lolD"
                     /locus_tag="OQQ48_002008"
     CDS             complement(6512..7213)
                     /gene="lolD"
                     /locus_tag="OQQ48_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HCS1952791.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(7206..8405)
                     /gene="lolC"
                     /locus_tag="OQQ48_002009"
     CDS             complement(7206..8405)
                     /gene="lolC"
                     /locus_tag="OQQ48_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HCS1952792.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSINPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRMMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGNEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEDSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMIFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPMAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            8667..9740
                     /locus_tag="OQQ48_002010"
     CDS             8667..9740
                     /locus_tag="OQQ48_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCS1952793.1"
                     /translation="MKQKELWINQIKGLCICPVVIYHSVITFYPHMTTFPHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            9868..13314
                     /gene="mfd"
                     /locus_tag="OQQ48_002011"
     CDS             9868..13314
                     /gene="mfd"
                     /locus_tag="OQQ48_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCS1952794.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTKHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDVARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            13458..14420
                     /gene="ldtC"
                     /locus_tag="OQQ48_002012"
     CDS             13458..14420
                     /gene="ldtC"
                     /locus_tag="OQQ48_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HCS1952795.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     TRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(14503..14760)
                     /gene="bhsA"
                     /locus_tag="OQQ48_002013"
     CDS             complement(14503..14760)
                     /gene="bhsA"
                     /locus_tag="OQQ48_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HCS1952796.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISAKAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            15001..15633
                     /gene="comR"
                     /locus_tag="OQQ48_002014"
     CDS             15001..15633
                     /gene="comR"
                     /locus_tag="OQQ48_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HCS1952797.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(15695..16234)
                     /locus_tag="OQQ48_002015"
     CDS             complement(15695..16234)
                     /locus_tag="OQQ48_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCS1952798.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(16461..17765)
                     /gene="ndh"
                     /locus_tag="OQQ48_002016"
     CDS             complement(16461..17765)
                     /gene="ndh"
                     /locus_tag="OQQ48_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HCS1952799.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHSELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTHDPDIYAIGDCASCTRPEGGFVPPRAQAA
                     HQMATCAMNNILAQINGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(18165..18707)
                     /locus_tag="OQQ48_002017"
     CDS             complement(18165..18707)
                     /locus_tag="OQQ48_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCS1952800.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(18730..19755)
                     /gene="nagZ"
                     /locus_tag="OQQ48_002018"
     CDS             complement(18730..19755)
                     /gene="nagZ"
                     /locus_tag="OQQ48_002018"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HCS1952801.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPEKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSTLIRENKLDAIMPAHVIYSD
                     VDPRPASGSSYWLKTVLRQELCFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKASSTRLNQLHERW
                     QEEKAGH"
     gene            complement(19766..20590)
                     /gene="thiK"
                     /locus_tag="OQQ48_002019"
     CDS             complement(19766..20590)
                     /gene="thiK"
                     /locus_tag="OQQ48_002019"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HCS1952802.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDTALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(20574..21212)
                     /gene="lpoB"
                     /locus_tag="OQQ48_002020"
     CDS             complement(20574..21212)
                     /gene="lpoB"
                     /locus_tag="OQQ48_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HCS1952803.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDRTAPPAPHIRHYDWNGAMQPIVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQ"
     gene            complement(21226..21603)
                     /locus_tag="OQQ48_002021"
     CDS             complement(21226..21603)
                     /locus_tag="OQQ48_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HCS1952804.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQKPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(21606..21965)
                     /gene="hinT"
                     /locus_tag="OQQ48_002022"
     CDS             complement(21606..21965)
                     /gene="hinT"
                     /locus_tag="OQQ48_002022"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HCS1952805.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVVAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
BASE COUNT         5604 a         5899 c         5575 g         5392 t
ORIGIN      
        1 gacgagtgga aaccaaaacg gttcagtctc ccaataacaa acactaccct ggtgaaatac
       61 ggaaaactag gatacatcgt tccaagacca caaaaaattc gtgggcgttg gctgatagat
      121 cgccgagcag tatttgttgg gccaggtgaa acgggaattg cgccggaaat tcatactggc
      181 gatgatgatg cactgaagga gattttaact catgtcaccg aggccacgaa aaaacagcac
      241 tgacgtagcc ggtctttacg aaaagtttga tcgcagaact ggcagagttt actaccagta
      301 taaaaatcct gtgactggaa aatttcacgg actcggaaca gacaaaggta aggcagaaaa
      361 aatcgcttcc acagccaatc agcgaatagc tgcagcagaa gctgaatatt tcatgcgcaa
      421 aattgatgaa agtccgtcag caacaaaacg tcggggtatc agattaaagg catgggttga
      481 tcgatatctg aaaatacagg acacgcgact gaaaaatgga gatattgcag ctacaactca
      541 caaagaaaaa actcgaatgg ctgcatacct ggtttcccgt ctgggaaacc acccattgaa
      601 agaactggaa gtaagagact ttgcattaat actggatgag tggctggata aagacatggt
      661 cagcacagcg agagtaaatc gtggattatg ggttgatatt tataaagaag cacagcatgc
      721 aggggaagtt cctcctggat ggaatcctcc ggaggctacc cgtaaaccga tccctaaagt
      781 aaccagagcc aggctcacca tggaagactg gcaaaaaatt tacaatgcaa cgcctgaaaa
      841 acactttatc cgtaacgcaa tgcttcttgc gattgttact ggtcagcgcc gtgatgacat
      901 ttgccacatg cgtttttcag atgtgtggaa cgaacacttg catatcaccc agggaaaaac
      961 cggaatgcgt ctggcgttac cgcttacact acgctgtgat gccattggga taacgttaaa
     1021 agaagttatt gatgggtgcc gagacagaat attaagtcca tatctaatcc atagtcggca
     1081 ccagaaacaa ccgaagccga tgagtaaaga caacctgagc gactactttg ccaaagcacg
     1141 ggatctggct gggataattc caccagcagg aaaaactccg ccaacatttc atgaacaacg
     1201 ctctctatca gaacggctgt accgtgcaca gggtatcgat acaaaaacat tactaggaca
     1261 taaagtccag gcaaccaccg atcgctataa cgatactcga ggtcaggaat gggtttaagc
     1321 tggttatttg acaaacacca tatggaatat ccgtcaccat ctcaggaaaa aagtgttgta
     1381 aaatgcgtgg cctagttttg gggaaacgtt ttggagagat tttggagaaa ggaaaaaatt
     1441 gaataaattc aaaatcctga aagtgctcca ccttatgaat aagaaggtgg aactcgaatg
     1501 atcggtcgac tgcttcgcgg cggttttatg accgctatct acgcgtacct gtatatccca
     1561 atcattattt tgattgtgaa ctcctttaac agctcgcgct ttggcatcaa ctggcagggt
     1621 tttaccacca aatggtatag cctgctgatg aacaacgaca gcctgttaca ggcagcgcag
     1681 cattcactaa caatggcggt gttttcggcg acgtttgcta cgcttatcgg ttcactgacg
     1741 gcagttgcgc tgtaccgtta tcgctttcgc ggtaagccgt tcgttagcgg aatgctgttt
     1801 gtggtaatga tatcaccaga tatcgtgatg gcaatttcgc tgctggtgct gtttatgctg
     1861 ctgggtattc agcttggctt ctggtcgctg ctgttctcgc atatcacctt ctgcctgcca
     1921 tttgtggtgg tgacggtgta ttcgcgcctg aaaggttttg acgtgcggat gctggaagcg
     1981 gcgaaagatc tcggtgccag cgaatttaca attctgcgaa aaatcatact gccactggca
     2041 atgccagcgg tggcagcggg ctgggtgtta agctttaccc tgtcgatgga cgatgtggta
     2101 gtttcttcgt tcgtcaccgg accaagttat gaaattctgc cgttaaaaat ttattcgatg
     2161 gtcaaagtcg gcgtatcgcc ggaagttaac gcgctggcaa ccatattact ggtgctgtcg
     2221 ctggtgatgg ttattgccag tcagcttatt gctcgtgata aaacgaaagg taacacaggg
     2281 gacgttaaat gaaaaaatgg tcacgccacc tgctcgcggc gggtgctctg gcactgggca
     2341 tgagcgccgc tcacgccgat gacaacaaca cgctgtattt ctacaactgg accgagtacg
     2401 tgccgccagg actgcttgaa cagttcacca aagaaacctg tattaaggtt atctattcga
     2461 cttacgagtc gaacgaaacc atgtacgcca agctgaaaac gtacaaagac ggtgcgtatg
     2521 acctggtggt tccttcaacc tattacgtcg ataaaatgcg taaagaaggg atgatccaga
     2581 agatcgacaa gtcgaagtta acgaacttca gcaatctcga tccagacatg ctcaacaagc
     2641 cttttgaccc gaataacgac tactccattc cgtatatctg gggggcgacg gcgattggtg
     2701 ttaacggtga tgcggtggat ccgaaatctg tcaccagctg ggccgatctg tggaagccag
     2761 agtacaaagg cagcctgctg ttgaccgacg atgcccgtga agtgttccag atggcgctgc
     2821 gtaagctggg ctattccggt aacaccaccg atccgaaaga gattgaagct gcatataacg
     2881 agctgaaaaa actgatgcca aacgtcgcag cgtttaactc cgataacccg gctaacccgt
     2941 acatggaagg cgaagttaac ctcggcatga tctggaacgg ttctgctttc gttgcacgcc
     3001 aggcgggtac gcctattgac gtggtgtggc cgaaagaagg cggcatattc tggatggaca
     3061 gcctggcgat cccggcaaat gccaaaaaca aagaaggcgc gctgaaattg atcaacttcc
     3121 tgctgcgccc ggatgtggca aaacaggttg ctgaaactat cggttatcca acgccaaacc
     3181 ttgcggcgcg taagctgtta agtccagaag tggcgaacga taaaacgctc tacccggatg
     3241 ctgaaaccat taaaaatggc gaatggcaga atgacgttgg cgcagccagc agcatttatg
     3301 aagagtatta tcagaagctg aaagcaggac gttaattcag caagcgggcg gtggtattac
     3361 cgcccgtttt ttatttagca aggatgtggc taatgagtca ggacagtaga gttttatccg
     3421 actctttcgt cggtattgtg gtccgctcgt aggcctgata agcgtagcgc atcaggcaat
     3481 gcttcctgct ttataatccc ttcagcaact tttcaacaaa ctcaggcacc acctggcttg
     3541 ccgggccgta atatttctcg gcaaattcat taccgacctg acttggttcg agattcagtt
     3601 ccacggtgtg cgcgccatgc agtttcgctt cgtgaacaaa cccagccgcc ggataaacat
     3661 gcccggaagt accaatggca atgaaaatat cagccatcga caacgccata taaatttcat
     3721 ccatgccgag tggcatttcg ccaaaccaaa cgacgtgcgg gcgcaagggt gccgggaact
     3781 ggcaacaatg gcatttatct tctggcgtaa cgtctccggt ccagtcgaga acctgaccac
     3841 tttgcgaaca acgaactttc agcaattccc catgcatatg aatgacattg gtattacctg
     3901 cgcgttcatg caggttgtct atattctgcg ttaccagcaa aaagcgatcg ccgagagcgt
     3961 cttgcagttt agccagcgca agatgcgcgg cgttaggctg aatttttggc tgctgcagct
     4021 gtcgacgacg ggcgttataa aacgcttgca ccagttcagg atcgcgatcg aaaccttccg
     4081 gcgttgccac atcttcaacc cgatgttctt cccacaggcc atctgcggcg cgaaaggtac
     4141 gaatacctga ttccgcagaa attcctgccc ctgtcagtac gagtactctt ggtttttcca
     4201 ttgcttccgg caccacttta tctctgaaaa aaatacgctg acgcaaacgc tcgcgcaggc
     4261 ggcgtttatt tttacgaaaa cgacttaacc gatgaccccg acgcgacagc atagcaacct
     4321 ctgttgttta atcggttaga tgtaggaagg ccgcaccacg cattcctccc gcatcaccgt
     4381 ggcgcgcgcg ttcaatgcgc ggaacacgag ctacaggtaa gagatgacga ggcagcctgt
     4441 ccgccagttg cgttgtgatt gccgggaaat tcgataagcc accaccaatg acgaccaggt
     4501 cagggtcaac aatggtcagg atatttccca gacaaaccgc taataaatcc agataacgct
     4561 caacgtgcgc ccttgcctgc tcatcgcctt gattataaag cgcaataatt tcgggagcct
     4621 gcaactgttg atgataatag tgttgataca gccacgcaaa accgcgacca gacagataat
     4681 tctcaatgca gccatgctga ccacagccgc agcggcgtaa cgggaaatcc aaccccatca
     4741 tggttaacgc atcaaccggc agacgcatat ggccaaactc gccggtaatg tagcttttac
     4801 cggtaatcgg tttgccgttg aaaatcagcc caccgccaac gccggtacca agaatcaacc
     4861 ccatcaccag tggatattgc gtaaattcat catcccaggc ttctgaaagg gcaaaacagt
     4921 tggcatcgtt atcaaggcgt acatcgcgat caagacgtgc gctcaggtcg gcacgcagcg
     4981 gtttaccgct ggcagcgggg acattggcgg catacagcgt accatcttct gtttccggca
     5041 ttcccggtat accaatgccg acagaacctc tacagccaaa acgttgatca gcttcggcta
     5101 ccagctcgca cactgcatct aaaaatgcgt catagctgtc acgcggtgtc ggcacccgct
     5161 tttcccactg caactgccga ccgctatcaa acacgccaag cgcaattttt gttccaccaa
     5221 tatcaaaccc gtaatacatt gccgctcctt gttttaatgt actgccttta ctggccgcta
     5281 aggactcgcg cagggtcaat attactggcg cgccgcgccg gataccaact tgccaaaaga
     5341 ctcagcaaca atgctgtgac cagtacgtag aagacgtcca gccagtgcaa ttccgatggc
     5401 aggaagtcaa taaaatagat atcgctggag aggaactgat gaccgatcag cttttcaatc
     5461 cgctcaataa tcggggtaag ttgcagtgaa acaactacgc cgataatcag cccacacagg
     5521 ctgccgaaca gccctgccag caatccatac cagacaaaga tggcgcgaat taaaccatct
     5581 ttcgccccca gcgttcttaa tactgcgata tcgccactct tatctttcac cgccatcact
     5641 aaggtggaga cgatgttgaa acaggccacg ccaatcacca gtaccatcgc cagatacata
     5701 atggcgcgga tcatctggat atcgcgatac atatagccgt aagtaccaat ccagctttta
     5761 atataaacat agctgttggt catttcaccc gcatcgcgta ccagcttatt ggcgttgaaa
     5821 acatccgtca ttttaagggc aatacctgac acgctggaac ccatatcaag atattgttgg
     5881 gcatccgcca gcgggatcat ggcaaaactg tgatcgagtt ggccactcaa ctgcaaaata
     5941 cccgcaacgt gcaaacgcac acgttttggc tgcatcagtt tatgctcagg attcgagttg
     6001 gggatcatga tcgacaccca atcgccctgc ttcactttca gcgcatccgc cacgcctttg
     6061 ccgatgataa tttgctgttc gcccgcttta aaattgcgcc acgcatcccc ctgaacaaac
     6121 gagggtaatg cgctcagacg ctgttcctgt tgtgggttaa cgcccttcac ctggattgcg
     6181 cgcagattcg ctccactttc caccagcccg gtgaaattga tatacggcgc agcggcggca
     6241 atacccggca ctttctgcac tttatccagt gcttcctgcc agttagtcca tggctgattt
     6301 accgcttcga tttcaccatg cggcaccacc gccagaatgc ggttattcag ttcgcgttca
     6361 aagccgttca tcgcgcttaa gccgacgatc aataccgcca caccgagggc aatgccaatg
     6421 gtagaaatga cggagatcag cgacaccatg ccgccgcgcc gccgtccgcg actaaaacgc
     6481 aggccaatta ataacgataa aggcatcgcc attactccgc ccccatcagg ctcagttccg
     6541 ccgtcagacg tccatcacgc atttctaact ggcggctcat acgtttcgcc agttgcaggt
     6601 cgtgagtaac caccaggaag gcggtgccct gcaagcgatt caattcccca agcaactgga
     6661 agatgctgtc ggcgttacgc gcatcgaggt taccggtagg ctcatccgcc agtaccagac
     6721 gcggattatt gaccagcgca cgggcaatcg ctacacgctg gcgttcgccg ccagaaagtt
     6781 cagatgggcg gtgattcgca cgatgctcca gccccaccgc ttttaacatc tccagtgcac
     6841 ggctgttgat ttcagcgggc tttttcttgc caatcagcag cggcatagcc acgttttcca
     6901 gggcggtaaa atccggcagc aggtggtgaa actgataaat aaagcccagt ttctggttgc
     6961 gcagttctgc ttttgctgcc gacgacagtt tgctcatcgg ttgaccgtta aagatcacat
     7021 cgccggaggt tggcgtatcc agtccgccca gcaggtgcag caaggtgctt ttaccggaac
     7081 cagagctacc gacgatcgcc atcatttcac cttcgccgac gctgaaactg acattgtgca
     7141 gtacatcggt ttgcacactg ccttcctgat agcgtttgca caggttgtcg cattgcaaca
     7201 ggatcttatt cataacgtaa agcctcagcg ggttgtgtgg cggcagcgcg ccatgaaggg
     7261 taaagcgtag acagcagcgc gatagccatc gccaccagcg caataacaat gacctgtaaa
     7321 ggttcgatag ccatcggcag cgccgcgcca tcaagcagga cgccgattat tggcatcaga
     7381 ttatttaact ggctggcaag cagtgcgcca agcgccgctc cgaggatcgc accgataatc
     7441 ccggcgctgg ccccttgcac cataaagatc atcatgattt gtcgcggagt taagccttgc
     7501 gtttgcagga tcgctacttc gccctgcttc tccatcacca tcagccccag cgaggtaata
     7561 atgttaaacg ccgcaacggc gacaatcagg ctcagcagta aacccatcat atttttttcc
     7621 atgcgtacgg cctggaacaa ctcgccttta cgatcacgcc agtcctgcca tttgctgtct
     7681 tcaggcagtt tttgctgact taatgagtcg actttcagcg gctcatccag ccacaaacgc
     7741 cagccggtaa tattgcctgc cggataacgc atcagacgcg aggcatcctc aatattcacc
     7801 agcatttcat tgccatcgac ttcactgttg gcggcgaaag tgccaatcac attgaacagg
     7861 cgctgacttg gaatacgccc catcggcgtg aactggctgg cagatggtac catcatgcgg
     7921 atttgatcac cgcgattaac gcctagctgt gaggcaagtt gttcgccgag gatgacatta
     7981 tatttccccg gctcgaggtc agtttgtttc acattgacca gatacggcgt tagtggatct
     8041 ttttgcgccg gatcgatacc cagcatcacc ccgaccgcca cgctgcgcgc gctttgcagt
     8101 accacatcac cggtagtaat aggtgcgacg cgattaacgc cgtccagttt gactgccgtt
     8161 tccgggagtt gctgcgggtt aatagagcca tgctcagaag agagaattgc ctgtggcatc
     8221 aggccaagga tgttgttttg cagctcgcgc tcaaagccgt tcatcactga caatactgtg
     8281 accagcgcca tcaccccgag ggtaatgccg atggtagaaa gccaggagac gaaacgaccg
     8341 aagcgatccg ctgcacgccc acgcatgtaa cgcaggccaa taaatagagc gacaggttgg
     8401 tacatgaaat ccgtctggtt gctgtagcaa agacacgagt atataagcga aagcaagatg
     8461 ggtaaacgtc tgaactgtaa gtgatcgtca gggctaatct gaaaagcccc cgtaaatcaa
     8521 tcggctaaat tgccatgttt tatcatcaat cttgccgcct gatcttaatc tgaaaaatca
     8581 tccaatacag acagataccc attacacctc gggtcataac ccgcaggatc aaccgcatgt
     8641 aaattaaaaa agagcgtgtt gtcctgatga aacaaaaaga gctatggatt aaccagatca
     8701 aagggttatg tatttgtccg gtggtgattt atcactcggt cattaccttt tatccgcata
     8761 tgaccacttt tccgcatccg ttatcggaag tcctgagcaa atgctggatc tatttcaatc
     8821 tttaccttgc accctttcgt atgccggttt ttttctttat atccggctat ttgattcgcc
     8881 gctatatcga cagcgtaccg tggggaaatt gtctcgataa acggatctgg aacatcttct
     8941 gggtgctggc gctttggggc gtggtgcagt ggctggcgtt aagtgcgctg aaccagtggc
     9001 tggcacctga gcgcgattta agcaatgcct ccaatgccgc ttatgccgat tccaccggtg
     9061 agttcctgca cgggatgatc accgccagca ccagcttgtg gtatctgtat gcgttaattg
     9121 tctatttcgt ggtatgtaaa atttttagcc gtctggcgct gccactattc gccttgtttg
     9181 tactgctgag tgtggcggtt aatttcgttc ccacgccgtg gtggggaatg aacagtgtga
     9241 tccgcaattt gccttattac agccttggcg catggtttgg cgcaacaata atgacctgtg
     9301 ttaaagaggt accgttgcgc cgccatctgc tgatggcttc tttgcttacc gttctggcgg
     9361 tcggtgcctg gttgtttact atctcgctgc tgttgtcgct ggtatcgatt gtggtgatca
     9421 tgaagctgtt ttatcagtac gagcaacgat tcggtatgcg ctccaccagc ctgctgaatg
     9481 tgattggctc caacaccatt gctatctaca ccacccatcg cattctggtt gaaatattca
     9541 gcttaactct gcttgcgcaa atgaacgcag cacgctggtc gccgcaagtc gaactgacac
     9601 tcctgctggt ttatcccttt gtcagtttgt tcatctgtac tgttgcgggc ttgctggtaa
     9661 gaaaactttc acagcgtgca ttcagcgatc tgttgttctc cccgccttca ctgcccgctg
     9721 ccgtcagtta ctcccgctaa gccatcgccc cagccggggc gattcaattt gctaaaccat
     9781 gtcgattacg gataatgaac agcattgctt atcagagata tgcccccata tgttgaggca
     9841 tatcctaacg agaatctgac aaccgttatg cctgaacaat atcgttatac gctgcccgtc
     9901 aaagcgggtg agcagcgtct gctgggcgag ttaaccggcg cagcctgtgc aacgctggta
     9961 gcggaaattg ccgaacgtca cgccggtccg gtggtactca ttgcaccaga tatgcaaaat
    10021 gctctgcgtt tgcatgatga aatcagtcag ttcaccgatc agatggtgat gaatctggcg
    10081 gactgggaaa ctcttcccta cgacagcttt tcgcctcatc aggacattat ctcctcgcgc
    10141 ctttccaccc tttaccagct accgacgatg cagcgtggcg tactgattgt tccggtgaat
    10201 acgcttatgc agcgcgtttg cccacacagt tttctccacg gtcatgcgct ggtgatgaaa
    10261 aaaggtcagc gcctgtcacg agatgcatta cgaacccaac tggacagcgc cggttatcgc
    10321 catgttgacc aggtgatgga gcacggcgaa tacgccacgc gcggcgcgtt gctggatctc
    10381 ttcccgatgg ggagtgagct gccttatcgt cttgatttct ttgatgatga aatcgacagc
    10441 ctgcgggtgt ttgacgtcga cagccagcgc acgctggagg aagtagaagc gatcaatctg
    10501 ctgcccgcgc acgaatttcc gaccgataaa gcggcaattg aactgttccg cagccagtgg
    10561 cgcgatacct tcgaagtgaa gcgcgatccg gaacatattt accagcaagt gagtaaaggc
    10621 acattacctg ccgggatcga gtactggcag ccattgttct tcagcgaacc actgccgccg
    10681 ctgttcagtt atttccctgc caataccttg ctcgtgaata ctggcgatct ggaaaccagt
    10741 gccgaacgtt tccaggctga cacgctggcg cgttttgaga atcgcggcgt cgatccgatg
    10801 cgcccgctgt tgccaccaca atcgctctgg ctgcgggtgg acgagctctt ctcagagctg
    10861 aaaaactggc cccgggtgca gctaaaaact aaacatttac cgacaaaagc cgcgaatgcc
    10921 aatttaggtt tccagaaact gccagaccta gccgttcagg cgcaacaaaa agcgccgctg
    10981 gatgcgctgc gtaagttcct cgagactttc gacggtccgg tggtgttctc ggtagaaagt
    11041 gaaggtcgcc gtgaagcgct gggtgaactg ctcgcacgaa ttaaaattgc tccgcaacgc
    11101 attatgcgtc ttgatgaagc cagcgaccgt gggcgttatc tgatgattgg cgctgccgaa
    11161 catggttttg tcgataaggt gcgtaatctg gcgctgatct gcgaaagcga tctgctcggt
    11221 gaacgtgttg cccgccgtcg tcaggattct cgccgcacca tcaaccccga tacactgatc
    11281 cgtaaccttg cggagctgca tattggtcag ccggtggtcc atctggagca cggtgtcggg
    11341 cgctatgccg gaatgaccac gctcgaagcg ggcggcatta ctggcgagta tttgatgctc
    11401 acctatgcca acgacgccaa actgtatgtt ccggtgtcgt cactgcatct gattagccgt
    11461 tacgcaggtg gcgcggaaga aaacgccccg ctgcataaac ttggcggcga tgcgtggtca
    11521 cgcgcgcggc agaaagcggc ggaaaaagtg cgtgatgtgg cggcggaatt gctggatatc
    11581 tacgcgcaac gcgccgccaa agagggcttc gcgtttaaac acgatcgtga gcagtatcag
    11641 ttgttctgcg acagcttccc gtttgaaacc acgccggatc aggcgcaggc cattaatgcg
    11701 gtacttagcg acatgtgtca gccgctggca atggatcgtc tggtgtgcgg cgatgttggc
    11761 tttggtaaaa cagaagtggc gatgcgcgcc gctttcctgg cagtagataa ccacaagcag
    11821 gtggcggtgt tggtgcctac cacccttctc gcgcagcagc attacgacaa cttccgcgac
    11881 cgtttcgcca actggccggt acgtatcgaa atgatctccc gtttccgcag cgccaaagag
    11941 cagacgcaaa tccttgcgga agtggcggaa gggaaaatcg atattctgat cggtacgcac
    12001 aaactgctgc aaagtgacgt caagtttaaa gatttaggcc tgctgattgt cgatgaagaa
    12061 caccgcttcg gggtgcgtca taaagagcgc attaaagcga tgcgcgcgaa cgtggatatt
    12121 ctgacgctta ctgcaacgcc gatcccacgc acgctgaata tggcaatgag cggaatgcgt
    12181 gacctgtcga ttatcgccac gccgcccgcc cgtcgtctgg cagttaaaac ctttgtccgt
    12241 gagtatgaca gcctggtggt ccgggaggcg atcctgcgtg aaattttgcg cggggggcag
    12301 gtttattatc tctacaatga tgtggaaaac atccagaaag ctgccgaacg gctggcagaa
    12361 ctggtgccag aagcgcggat cgccatcggt cacgggcaga tgcgcgagcg cgaactggaa
    12421 cgggtgatga atgatttcca tcatcaacgt ttcaacgtgc tggtttgtac aaccattatc
    12481 gaaaccggga tcgacatccc gacagccaac accattatca ttgaacgcgc ggatcacttc
    12541 ggtctggcgc agctgcacca gttacgcggt cgcgtcggac gttcacatca tcaggcatat
    12601 gcatggctgc tgacgccgca tccaaaagcg atgactaccg atgcacaaaa acgtcttgaa
    12661 gcgattgcct cgctggaaga tctcggtgca ggctttgcgc tggcaacgca cgatctggag
    12721 atccgcggcg cgggtgaact gcttggcgaa gaacaaagtg gctcaatgga aaccatcggt
    12781 ttctcgctgt atatggagtt gctggaaaac gccgtcgatg cactgaaagc cggacgcgag
    12841 ccgtcgctgg aagatctcac cagccagcaa acagaagtcg agctgcggat gccgtcgcta
    12901 ttgccagatg atttcattcc tgacgtgaat acgcgtttgt cgttctataa acgtattgcc
    12961 agcgccaaaa cggaaaacga actggaagag atcaaagtcg agcttatcga tcgcttcggc
    13021 ctgctgccgg atccggcgcg taccctgctg gatgttgccc gtctgcgcca gcaagcgcag
    13081 aaactgggga tcaggaagct ggaaggtaat gagaaaggcg gggtgatcga atttgccgag
    13141 aagaatcacg ttaatccggc ctggttgatt ggtttgctgc aaaaacagcc gcagcattac
    13201 cgccttgatg gtccgacgcg cctgaaattt attcaggatt tgagtgagcg gaaaacgcgt
    13261 atcgaatggg tacgccagtt tatgcgtgaa ctggaagaga acgcgatcgc ttaatggtaa
    13321 atcgggggcg tttctgcgcc cccatcggca acatttacaa attctttgca cttccctgca
    13381 ctatccgaca ctgtcaccat ccataattca ggcttatctg tttattacaa taaccttata
    13441 tttattatgg atttttggtg atgatcaaaa cgcgtttttc tcgctggcta acgtttttta
    13501 cgttcgccgc tgccgtggcg ctggcgctac cggcaaaagc caacacctgg ccgctgccgc
    13561 cagcgggcag tcgtctggtt ggcgaaaaca aatttcatgt ggtggaaaat gacggtggtt
    13621 ctctggaagc catcgccaaa aaatataacg tcggctttct cgctctgtta caggctaacc
    13681 ccggcgttga tccttacgta ccgcgcgcgg gcagcgtgtt aacgatcccg ttgcaaaccc
    13741 tacttccaga cgcgccgcgc gaaggcattg tgatcaacat tgcggagctg cgtctctatt
    13801 actacccgcc gggtaaaaat tcggtaaccg tgtatccaat aggtattggt cagttaggtg
    13861 gtgacacgct gacaccgaca atggtgacca ccgtttcaga caaacgtgca aacccaacct
    13921 ggacgccaac ggcaaacacc cgcgcccgtt ataaagcaca gggaattgag ttgcctgcgg
    13981 tagtgccggc tggaccggat aacccaatgg gccatcatgc gattcgtctg gcggcctatg
    14041 gcggcgttta tttgcttcat ggtacgaacg ccgatttcgg cattggcatg cgggtaagtt
    14101 ctggctgtat tcgtctgcgg gatgacgata tcaaaacact ctttagccag gtcaccccag
    14161 gcaccaaagt gaatatcatc aacactccga taaaagtctc tgccgaacca aacggtgcgc
    14221 gtctggttga agtacatcag ccgctgtcag agaagattga tgacgatccg cagctgctgc
    14281 caattacgct gaatagcgca atgcaatcat ttaaagatgc agcacaaact gacgctgaag
    14341 tgatgcaaca tgtgatggat gtccgttccg ggatgccggt ggatgtccgc cgtcatcaag
    14401 tgagcccaca aactctgtaa atcctggata aaaaaaatcc ccgcgacacg gcatgtcgcg
    14461 gggaaaaaat cagcagtagc aggcattaat gagggttaat gcttatttat aaattactgc
    14521 tgttccatgg agggtattcg gaccggttac agaagtaata cggaaagatt ttgcacccat
    14581 ctcatccgct ttttgcgcca gctgctcttc cagcgatccc agatttgtcc ccgctttagc
    14641 actgattgta ccgacttttt gttggccttc tggcgttgac tgaacttcga cagccgcaaa
    14701 gctggcaaat gacatggagc ttaaaatcgc cgcagcgatg agggttttta cgtttttcat
    14761 aatagtggcc ttatgcagat gaatgacgtc gcaagcaatt acttaacgat cgataactaa
    14821 atgacagatg tgatctggat cacatacaag atatttttta taacaatcat taattaattt
    14881 aaaaatcttt aaatttcagt taaatagaaa gcaattattt ttatgggatc tggactggtg
    14941 aacggaggtg attattttta taataatctt tcattcacag tcaccaggta caacggcatc
    15001 atggcaactg actcaacaca atgtgtaaaa aaaagccgtg gccgcccaaa agtgttcgac
    15061 agggatgccg cgcttgataa ggccatgaaa ttgttctggc aacacggtta tgaagcgact
    15121 tctcttgcgg acctcgtcga agcgaccgga gccaaagcgc ccacgctata cgcggaattt
    15181 accaacaaag aggggttatt tcgtgccgtt ctcgaccgct atatcgatcg ttttgccgct
    15241 aagcatgaag cacagctgtt ttgtgaagag aaaagcgtgg agtctgcgct ggctgactat
    15301 tttgctgcca tcgccaactg ctttaccagc aaagacaccc cggcaggctg cttcatgatc
    15361 aacaactgca ccaccctctc cccagattca ggagatatcg ccaatacgtt gaaatcacgc
    15421 catgcgatgc aagagcgcac tttgcagcag tttttatgtc aacgacaagc gcgcggggaa
    15481 atcccgaccc actgtgacgt gacacatctg gcagaattcc ttaattgtat tattcagggg
    15541 atgtcgatca gcgcacgcga aggtgcatcg ctggaaaaac taatgcagat tgccggaacg
    15601 actttgcgtt tatggcccga actggtgaaa taagtcgcaa aaaaagcccc tcagcctgag
    15661 cgaatgaggg gccaaattgc agagtacagc cttgttatac tttgttattc aaaatcagtt
    15721 gcccattact atctagcggg atctgcgtac ccggatcgcg gtccatgcgg attttgccct
    15781 gctgatcgcc aatcttatag gtcacatcat aaccgagcat tttttctgac ttgtcataca
    15841 ccgttttaca acgctgttgc gtagtcgtgt aagtatcgct ttcctggaga gagccctgga
    15901 tctggttacc ggcatatcca ccacccagcg cccccacaac agtggcgaca tctttaccgc
    15961 gaccaccgcc aaactgatgc cctatcacgc cgccagcaac agcgccgagc accgacccgg
    16021 taatgcgatt ttcatcctgc accggtcgac gatgggtcac tgtgacgttg cgacactcct
    16081 gacgcggtgt tttaaccgtt tccttgattg gggttgcaga aacaacctga gcgtattgcg
    16141 ggccacgttc aaacacgttc agactggcca ctgccgctac gcccagcgca gctgcgacac
    16201 caatcccgat acccgccaac attgatttat tcacgggaac acctccttct ttgcagttat
    16261 gccaatcatg caagcgaagg agaaaagcag ctatcagaca gaagatgaaa agcgcgggaa
    16321 tggctggaaa tacttaatga ttcggaggag tctgaaggaa tgcctgatgc gacgcttaac
    16381 gcgtcttatc aggcctaccg ctcgtaccat ttgtccaggg cggatgaggc gtttatgcca
    16441 catccgccag tgtacgtcga ttaatgcaac ttcaaacgcg gacggataac gcggttaata
    16501 ctccccacca gcatcattaa tccggtttta aagtaaccat gcagcgcaat ctgatgcatt
    16561 cggtatagcg agatatatac aaagcgcgca attcgtcctt caatcatcat tgagccgcgc
    16621 gtcaagttac ccatcaggct accaacggtg gagaagttcg acagcgatac cagtgaacca
    16681 tgatctttat actgataatt tttcagcggc ttaccgttta tctgcgccag aatattgttc
    16741 attgcgcaag tcgccatctg gtgtgcagcc tgagcacgcg gcggaacaaa gcccccttcc
    16801 ggacgcgtgc atgacgcgca gtcgccaata gcgtaaatgt ctggatcgtg ggtggtttgc
    16861 agcgtcggtt ccaccaccag ctggttgata cggttagttt caagaccacc gatatctttc
    16921 aggaagtctg gcgctttgat cccggctgcc cacaccatca gatcagcctc aatatattca
    16981 ccatctttag tgtgcaggcc gccttcatca gcactggtga ccatggtttg cgtcagcacg
    17041 cgaacgccaa gtttcgttaa ctcactatgg gccgcagcag agatgcgtgg cggtaatgca
    17101 ggcaaaatac gttctcccgc ttctactagc gttacgttca gggcttcgtt ggtcagacct
    17161 ttataaccgt agctgtgcag ttgcttgacc gcgttatgca actcagcgga gagttctaca
    17221 cccgtcgcgc cgccaccgac aatcgcaatg ttcactttgc cattcgcgcc caggttggcg
    17281 gagtatttca ggaacagatt cagcatctcc tggtggaagc gacgcgcctg gtgcgggtta
    17341 tcgaggaaaa tgcagttctc tttgacgcct ggcgtattga aatcgttaga ggtgctaccc
    17401 agcgccatga ccagggtatc ataggcgatt ttacgttccg gaaccagcag ttcacctttc
    17461 tcgtcgcgta gttctgcaat agtgattgtt ttcgcttcac gatcaatatc aatgacggaa
    17521 cccaactgga actggaaacc atgattgcgc gcatgggcca gatagctcaa cgcatcgacg
    17581 ccttcatcaa gcgagccagt cgccacttcg tgcagcagcg gtttccacag gtggctgtgg
    17641 ttacgatcga ccagcgtaat tttggctttt ttcttacgtc ccagcttatg ccccagctgt
    17701 gttgccattt ccagcccacc agcaccgccg ccgacaatca caatcttttt caatggcgta
    17761 gtcaacgtga cccccttaaa tttattaacc aattgttaat taatggttat taacatagct
    17821 cttaattaac aacaggttac agcaatgaaa atgttcttcg ctattgagaa taacatgaat
    17881 ggtgcattgg tcataccaaa attgatgtta atcaagtttt gttgctgaaa agataggcga
    17941 ctttaccggg tgagatcaaa aaatagagtt tattgtttat tatttgagcg aatttgtgag
    18001 agtgatatag agtgaggggt gtatgcaggg cgttcggtag gcctgataag aggcgcatca
    18061 ggcattgatg ctcgactgtc ggatgcggcg taaacgcctt atccgactta cagaattcgg
    18121 agcgcatcag gtaccggcaa ggttacctga tgcgacgagg gcatttaccc gagggttttg
    18181 aacgctttaa tgcgctgcaa atgcggggag atattcttga atttgtgcgt ctgctcttcg
    18241 tcccagacaa tctcgtaata atgatgcaac tcttcagatg tccgctggct gttaagcgct
    18301 tcatcattac gcgacaaaat caccaggcaa cgatcgcgat tcttctcgcg gaagttggtc
    18361 acacacttag tggcaatatc ggcatactct tccgggcgat caatcttgcc ttccatgttc
    18421 tcataaggga acaaattagg gttgaagatc acctggcgga tatcgcaaag aaaaccaatc
    18481 cgttccgccc agtatccgcc cagaccaacg ccgcaaatca gcggacgctc gtcgacgttc
    18541 agttgcaaca ttttgtccac ttctttaagc agatgctgca tatcatgttt cggatgccgc
    18601 gtactgtagc ttatcaagcg tacatccggg tcaataaact gcaattgtaa gactttctcg
    18661 tggttacccg gactgttaga gtcaaaaccg tgtaaataga taatcatcgc ttcctcacat
    18721 aagccagggt tagtgacctg ctttctcttc ctgccagcgt tcatgtaact gattcagacg
    18781 ggtgctgctc gccttccagc gagccgagtc catcagttcc tgtcgcgaaa atgaaccttt
    18841 atgatacaaa cgtgtaacac gttctgcctt gatcggtgac agattatcta acacgctgac
    18901 ggccccttta cgattattgc agaccaggat catatcgcaa cctgcgtcca gtgatgcctg
    18961 cccgcgttcg gcataactgc ccataatcgc ggcaccttcc atcgataaat cgtcagagaa
    19021 aattacaccg tcaaaacaca gttcctgacg caaaacggtt ttcagccagt aagaagaacc
    19081 gctcgccgga cgcggatcaa catcactgta gatcacatgc gcaggcataa tggcgtcgag
    19141 tttattttcg cggattaacg tgctgaaaac cgacatatct ttggcacgaa tttccgcttg
    19201 cgggcgtggg tcgcacggcg tctctttgtg tgaatctgca gttactgcac cgtgtcctgg
    19261 gaagtgtttc ccggtcgttt tcattccggc ttcatgcatc ccatcaataa accgactagc
    19321 gattgccagg gctttttctg ggtcggcatg ataagaacgc tcgccaatcg ccgcgctgat
    19381 atgtcctaca tccagcactg gcgcaaagct gatatcaata tccatagcga tcatttcgct
    19441 ggccatcagc caacccgcct cttgcgccag tttgccgccc tcttccattc ccaacagcgc
    19501 agcaaaggat tgtgctgccg gtaagcgggt aaaaccttcg cgaaaacgct gcacgcgtcc
    19561 accttcctga tctaccgcca ccaccagatg attgcgcgat gctgcgcgga tctggcgcac
    19621 cagttcacgt aactgggcag gatcatgata gttacgcgta aagagaatca gccctcccac
    19681 cagcggatgc gccagtattt cacgctcttc cgcgtccagt tcataacctt cgacatccaa
    19741 cattactgga cccacactgc tctccttatt gttttattaa tagctgccgc caggtgtcat
    19801 cggccagcct gataaattgt tgatcgccgg tttgtcgcca gcggtactca aaccaccctg
    19861 ctttgagcat cagcagccag ggaaaccatc gcctgacctg acgccataat tgcgccggat
    19921 aaatcttcgc gcgagtggca tagtcattga ccaattgccg gtgctgttca gtattttcca
    19981 cccacaccgc cgccagttcc agcgcggtat caccatctcc ggcatactcc cagtcgatga
    20041 gttttaaccc tgatgcgcta tgcactaaat ttccggcgtg gacatccata tgcaatggac
    20101 ttaagcgtaa aggtcgtggt tcccgcgctt tgcgcagacg ttttaacatt cgcagccaac
    20161 ccacagtccg ccgcgccgga tcgctttgct gccagtacag ttccagtaac ggcaacagcg
    20221 ttattcgcca gccaaaacgt ggttgttgat gtagataata cagcaagcct gccagttcgt
    20281 tggtatccgg caaatacgtt tttaccgcgc cgggcagata gtcgactacc atccagtcac
    20341 ggagatataa atgcggcttc ggtgcaatgc ttgcgggtag ttgtgataaa gcccgatact
    20401 ggcgcaagaa cgcggactgc ggcgcatcag gatcgtgcgg ctgacgcaca acaaaacgct
    20461 ggccctgatg ttcaatgaga aaactaccgc cactaagccc actattaaac gtcgtgacgg
    20521 gatgaaactg cgggaaaaag cgcgacagca attcgtcgcg cgtgatggga ttattactgc
    20581 gaaacggcac ctttacctga ccagataatt tcgcccgtct gcaccagcat cagctgcatt
    20641 tgtagggtcg gagcgttaac gttgccagag gcgctggagt acagcacgta atgagcgccg
    20701 acattgcggg caatgcctat ggctttacta cgggtgccta aactgtcctg cggcgacaaa
    20761 cctaactgtt gcttagccat cgacagttgc tgggcggaaa ccagggtaaa tttcccgtta
    20821 ttagccagcg catttcgcag cgtttcggtc gcttctgcgg cattcagcga accgttagta
    20881 cggttgttaa cgctatcaac cagcaggacg ctacccgcag tcaccccgtc agccccaagc
    20941 atcttactga ctatcggctg cattgcgcca ttccagtcat aatggcgaat atgcggcgca
    21001 ggcggtgccg ttcgatcttc gtgctcaatt gggcctggct gctgcgggat cgtcggcacc
    21061 gagggcactg tggggacagg ctgttgtggc tcggctggtt gttccggcgc tggtttcact
    21121 tcttctaccg gtgcaggttc acgttgcccc acacacccgg cgagaaacat cgccagcgcg
    21181 gtaatcaagg cgtagcgact catttttgtc atcaagattc accccttaca aatatagata
    21241 aagtctgact ttgtgcgccc ccagaaaatt ggcgctgccg tacagcgtta ccgccgaatg
    21301 tgcgggaatg gtaacgctgc gtggcctttc cagaggatgc atctccagcc ctctggcgtc
    21361 ataccagtaa aaacgataat gaacggtaac gggtttttgc ctttcgttat aaagcgttga
    21421 ggatgctgaa ggttgaatat cagacgtcga aaggacgggc ttttctgcac tgatgcccgc
    21481 agccaataaa gatgactcca tcaccagcga ttgctcatca ttcaccggaa tttccggatg
    21541 tgaacgacag cccaccagca gtaacaacac cagagacacc agcccaaagc atccttttct
    21601 catcgttaca gacctttatg cgccagcatt ggtcccagcg gacggccacc caacaagtgc
    21661 atatggatgt ggtaaacctc ttgtccgcca tggcggttgg tattcatgat cagacgatag
    21721 ccatcttcgg caataccttc ttgctcagca atttttgcca ctacggtgat catgcgtccc
    21781 agcgcctgct catgctcagc tgagacgtcg ttcacagtcg gaatgaggat attcggaatg
    21841 atcagaatat gcgttggcgc ttgcggcgaa atatcgcgaa acgccgttac cagatcatcc
    21901 tggtagacga tatcggaggg gatctcacga cgaataattt tgctgaatat agtttcttct
    21961 gccacgacat tttccttttt caaatagcca tgcgttgcgc catgctacgc cgcgccacac
    22021 tgcgagtata gagtatggtc gagttaaacc tgctctttca accttaactc acgatttctt
    22081 aagcaaaaaa tcgttttgct gccgtatata tcgccattat tcccatttct gcctgcgaat
    22141 gcgctgaaaa gacattctct gtttggttta cacgacaaat atgaatgcgt atatttctca
    22201 tttgcattta caaacaaaat tattcgcaca ctaaatataa tcgctcatca ccctcctccg
    22261 gcatgagcct gacgacaaca taaaccatga gatttcaaat gctttcaaca caatttaaca
    22321 gggataatca atatcaagcc atcaccaaac cgtcactact tgccggttgc atagcactgg
    22381 cactattacc ttctgccgct tttgctgcac cagccactga agaaacggtg attgttgagg
    22441 gttcagccac agctccagat gatggcgaaa
//
LOCUS       DAJVUJ010000059        22359 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.059, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000059 DAJVUJ010000000
VERSION     DAJVUJ010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22359
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.059"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..1298
                     /locus_tag="OQQ48_002023"
     CDS             <1..1298
                     /locus_tag="OQQ48_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HCS1952806.1"
                     /translation="WLELEGAKDLDDALSLLTILYHHVPSVTSMPVYLGQLDALLQPY
                     VRILTQDEIDVRIKRFWRYLDGTLPDAFMHANIGPSDSPITRAILRADAELKQVSPNL
                     TFIYDPEIPPDDLLLEVAKNICECSKPHIANGPVHDKIFTKGGYGIVSCYNSLPLAGG
                     GSTLVRLNLKAIAERSESLDDFFTRTLPHYCQQQIAIIDARCEFLYQQSHFFENSFLV
                     KEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAARYGKEAAANEVGYRISAQLAEFVA
                     NTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPYGDEPDPIIHLQTVAPHHAYYYSGI
                     SDILTLDETIKRNPQALVQLCLGAFKAGMREFTANVSGNDLVRVTGYMVRLSDLEKYR
                     AEGSRTNTTWLGEEATRNTRILERQPRVISHEQQMRFSQ"
     gene            1270..2133
                     /locus_tag="OQQ48_002024"
     CDS             1270..2133
                     /locus_tag="OQQ48_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HCS1952807.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVLWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQHLTGRDNQQIKRSICLLAERGKLAELRLLVIPDQ
                     VDYLHHIDELATFIKRLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(2347..3129)
                     /locus_tag="OQQ48_002025"
     CDS             complement(2347..3129)
                     /locus_tag="OQQ48_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCS1952808.1"
                     /translation="MICRFIDTHCHFDFLPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEVGLDL
                     FGDDPQLERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLSRTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAVRVFDVLCELRPEPEDEIAEVLLNNTYALFSVSG"
     gene            complement(3126..4199)
                     /locus_tag="OQQ48_002026"
     CDS             complement(3126..4199)
                     /locus_tag="OQQ48_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HCS1952809.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGCYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVIDAPQANDPTFNNEDLA"
     gene            complement(4321..4482)
                     /locus_tag="OQQ48_002027"
     CDS             complement(4321..4482)
                     /locus_tag="OQQ48_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HCS1952810.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(4609..5214)
                     /gene="osmY"
                     /locus_tag="OQQ48_002028"
                     /pseudo
     CDS             complement(4609..5214)
                     /gene="osmY"
                     /locus_tag="OQQ48_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
     gene            complement(5607..7196)
                     /gene="prfC"
                     /locus_tag="OQQ48_002029"
     CDS             complement(5607..7196)
                     /gene="prfC"
                     /locus_tag="OQQ48_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCS1952811.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     SRLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(7287..7964)
                     /gene="yjjG"
                     /locus_tag="OQQ48_002030"
     CDS             complement(7287..7964)
                     /gene="yjjG"
                     /locus_tag="OQQ48_002030"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HCS1952812.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGIAKPNKKIFDYAL
                     ELAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHHLEQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(7979..8425)
                     /gene="rimI"
                     /locus_tag="OQQ48_002031"
     CDS             complement(7979..8425)
                     /gene="rimI"
                     /locus_tag="OQQ48_002031"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCS1952813.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKNFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIVVDPDYQRQGLGRVLLKHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(8394..8807)
                     /gene="holD"
                     /locus_tag="OQQ48_002032"
     CDS             complement(8394..8807)
                     /gene="holD"
                     /locus_tag="OQQ48_002032"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HCS1952814.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            8910..9941
                     /gene="rsmC"
                     /locus_tag="OQQ48_002033"
     CDS             8910..9941
                     /gene="rsmC"
                     /locus_tag="OQQ48_002033"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HCS1952815.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATVDDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANCVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            10209..10295
                     /locus_tag="OQQ48_002034"
     tRNA            10209..10295
                     /locus_tag="OQQ48_002034"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10243..10245,aa:Leu,seq:cag)
     gene            10324..10410
                     /locus_tag="OQQ48_002035"
     tRNA            10324..10410
                     /locus_tag="OQQ48_002035"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10358..10360,aa:Leu,seq:cag)
     gene            10446..10532
                     /locus_tag="OQQ48_002036"
     tRNA            10446..10532
                     /locus_tag="OQQ48_002036"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10480..10482,aa:Leu,seq:cag)
     gene            complement(10571..10807)
                     /locus_tag="OQQ48_002037"
     CDS             complement(10571..10807)
                     /locus_tag="OQQ48_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HCS1952816.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYVLLPSCLALIAAIMLMIMNLNQG"
     gene            10948..11736
                     /gene="fhuF"
                     /locus_tag="OQQ48_002038"
     CDS             10948..11736
                     /gene="fhuF"
                     /locus_tag="OQQ48_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HCS1952817.1"
                     /translation="MAYRSAPLYEDIIWRTHLQPQDAGLAQAVRATIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            complement(11774..12450)
                     /gene="bglJ"
                     /locus_tag="OQQ48_002039"
                     /pseudo
     CDS             complement(11774..12450)
                     /gene="bglJ"
                     /locus_tag="OQQ48_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
     gene            complement(12408..13133)
                     /locus_tag="OQQ48_002040"
     CDS             complement(12408..13133)
                     /locus_tag="OQQ48_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCS1952818.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDF
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMNETHDFSYRSVILTLSERK
                     VLRLLGEGWGINQIVSLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
     gene            13752..14522
                     /locus_tag="OQQ48_002041"
     CDS             13752..14522
                     /locus_tag="OQQ48_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HCS1952819.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNKGGWDGAIITFFASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            14513..14986
                     /locus_tag="OQQ48_002042"
     CDS             14513..14986
                     /locus_tag="OQQ48_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HCS1952820.1"
                     /translation="MGVIEFLFALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTRGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            15094..15633
                     /gene="dnaT"
                     /locus_tag="OQQ48_002043"
     CDS             15094..15633
                     /gene="dnaT"
                     /locus_tag="OQQ48_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HCS1952821.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            15636..16373
                     /gene="dnaC"
                     /locus_tag="OQQ48_002044"
     CDS             15636..16373
                     /gene="dnaC"
                     /locus_tag="OQQ48_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HCS1952822.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            16422..16916
                     /locus_tag="OQQ48_002045"
     CDS             16422..16916
                     /locus_tag="OQQ48_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HCS1952823.1"
                     /translation="MMETVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            17170..19461
                     /gene="opgB"
                     /locus_tag="OQQ48_002046"
     CDS             17170..19461
                     /gene="opgB"
                     /locus_tag="OQQ48_002046"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HCS1952824.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRHRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWQGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(19600..20622)
                     /gene="lgoD"
                     /locus_tag="OQQ48_002047"
     CDS             complement(19600..20622)
                     /gene="lgoD"
                     /locus_tag="OQQ48_002047"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HCS1952825.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVRLGKNIADLKNGLQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPLLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            20761..21675
                     /locus_tag="OQQ48_002048"
     CDS             20761..21675
                     /locus_tag="OQQ48_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCS1952826.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTLINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            21890..>22359
                     /locus_tag="OQQ48_002049"
     CDS             21890..>22359
                     /locus_tag="OQQ48_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1952827.1"
                     /translation="MEKENITIDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHNFTQFVLVRIGMGIGEAPMNPCGVKVIN"
BASE COUNT         5525 a         5846 c         5531 g         5457 t
ORIGIN      
        1 aatggctgga gctggaaggc gcgaaagatc ttgatgacgc actctctctg ctgaccattc
       61 tttaccacca cgtaccgtcg gtcacatcga tgccggtcta cctagggcaa ctggatgcgt
      121 tgttgcaacc gtatgttaga attctaacac aagacgagat cgatgttcga ataaaacgtt
      181 tctggcgtta cctcgacgga accctgccag acgcctttat gcacgccaat atcggcccgt
      241 ctgattcgcc cattacccgc gcgatcttac gtgcagacgc agagttgaag caggtttcac
      301 cgaacctgac ctttatctac gatcctgaaa tcccccctga tgacctgctg ctggaagtgg
      361 cgaagaacat ctgtgaatgt agcaaaccgc acatcgccaa cggtccggtg catgataaaa
      421 ttttcacaaa agggggctac gggattgtga gctgttacaa ctcactgccg ctggcgggtg
      481 gtggcagcac gctggtacgc cttaacctga aagccattgc cgagcgcagc gaatcgctgg
      541 atgacttctt tacgcgcact ctaccgcact actgccagca gcagatcgcc atcatcgatg
      601 cgcggtgtga attcctctat caacaatcac acttctttga gaatagcttc ctggtgaaag
      661 aagggctgat taaccctgaa cgttttgtgc caatgtttgg catgtatggg ctggcggaag
      721 cggttaactt gctgtgtgag aaagaaggga ttgccgcgcg ctacggtaaa gaagccgccg
      781 caaatgaagt gggttatcgc atcagcgcgc aactggcgga gtttgtcgcc aatacccccg
      841 tgaaatatgg ctggcaaaaa cgcgccatgt tacacgcaca gtcggggatc agttccgata
      901 tcggcaccac gccgggcgcg cgtttgccgt atggcgatga gccagatccg atcatccatc
      961 tgcaaactgt cgccccgcat catgcttatt attattccgg catcagcgac attctgacgc
     1021 tcgacgaaac catcaaacgt aatccgcagg cgctggtaca gctttgcctc ggtgccttta
     1081 aagccggaat gcgtgaattt accgccaatg tcagcggtaa cgatctggtt cgcgttaccg
     1141 gttatatggt gcgtttgtcg gatttagaaa aatatcgcgc cgaaggttca cgcaccaaca
     1201 ccacctggct gggcgaagaa gccacacgca acactcgtat tctggaacgc cagccgcgcg
     1261 tgataagcca tgaacagcag atgcgcttta gtcagtaaga ttatcccctt ctcctgcgtt
     1321 gacgggccag gcagtcgtct ggctctgttt ctgcagggct gcaatctgcg ctgcaaaaac
     1381 tgtcacaatc cgtggacgat gggacgctgc aatgattgcg gagagtgcgt gccacagtgt
     1441 ccgcatcagg cgttgcagat tgttgacggc aaagtgctgt ggaacgctgt ggtctgcgag
     1501 cagtgtgata cctgcctgaa gatgtgtccg caacatgcca cgccaatggc gcaatccatg
     1561 agcgtggacg aagtgcttag ccatgtccgc aaagcggtgc tgtttattga agggataacg
     1621 gtaagcggcg gcgaagccac gacccaactg ccgtttgtag tggcgctgtt tactgctatc
     1681 aaaaacgatc cgcaactgcg ccacctcacc tgcctggtgg acagtaacgg catgttgagc
     1741 gaaaccggct gggaaaaatt actcccggtg tgtgacggcg caatgctcga tctcaaagcg
     1801 tgggggagcg aatgtcatca acacttaacc ggacgcgata atcagcagat taagcgcagc
     1861 atctgtttgc ttgcagagcg cggcaagctg gcggaactgc gtttgctggt aattccagac
     1921 caggtggatt atttgcatca catcgatgag ctggcgacgt ttatcaagag acttggcgat
     1981 gttccggttc gcctgaatgc gtttcatgcc cacggcgtgt atggcgaggc gcaaagctgg
     2041 gcgagcgcca cgccggaaga cgttgagccg ttggctgatg cgttaaaggt gcgcggggtg
     2101 agccggttga tatttccggc gctctatttg tgacatgccg gatacggcct acggttcggc
     2161 acagacttgt aggcatgata agacgcgaca agcgtcgcat caggcattgt gcgccaactg
     2221 ccggatgcgg cgtgaacgcc ttatccggcc tacggttcgg cacaaacctg taggcatgat
     2281 aagacgcgac aagcgtcgca tcaggcatta tgcgccgact gccggatact gcctacgaag
     2341 ggtaaactac ccactaacgc taaacaacgc atacgtatta ttaagcaaca cttcggcaat
     2401 ctcatcttct ggttccgggc gcaactcgca aagcacgtca aacacccgca cagcctgttc
     2461 cggtcggtta ggctgcccct gaaagccgtt gagcggcata tccggcgcgt cggtttccag
     2521 caacaacgat gccagcggta attttgcgat gacatcgcgg gttttactgg cgcgtggata
     2581 ggtgatagta ccgcctacgc cgattttgta gcccagctgt acaaaccgct cggcctgttg
     2641 caggctgccg gaaaaaccgt gaaccacgcc agtgcgcgat aaatcgtggc gtttaagatg
     2701 catcgccagt ttgtcgtgtg tgcgccgtga atgcaggatc accggcagat cgtagcgttt
     2761 cgccagtttc agctgttcgt cgagtaacca ctgctgcctc tcaagttgcg gatcgtcgcc
     2821 gaagagatcc agaccgacct cccccactgc caccaccttc gccggacgcc tttccagcgc
     2881 ctgctgtagc tgctcaagag acacatcgct atgtttttcc aacatacctg gatgcaagcc
     2941 caatgcggca tacagcggtt gatatttttc cgctaacgcc tgcacacggg caaaattttc
     3001 cgcctctgtc gccggaacaa tgatcttgcc tacgcccgct tgtgccgcgc gttgcaggct
     3061 ggcctcttca tcgccactaa acggcaggaa atcaaaatgg cagtgggtgt cgataaaacg
     3121 gcaaatcaag ccagatcctc attgttaaat gtcgggtcgt tcgcctgcgg agcgtcaatc
     3181 acttcggcaa ccagcgtatc gttggcgact ggcgcaggcg gaataacgat cgattccggc
     3241 gtcaccactg gcaccagatg gcgggtaagc ggcgcttttt cagttaatag cttgccaacc
     3301 gtggcgagga aataacaacc gcataaacgc ccaagtttat agtcttcacg cagcgccgga
     3361 atccgactgc caagcgcgat actatgtaat ggcttcggcg gataaatttc gaatatccgc
     3421 agcttgcccg gtggtttctc aataaattgc tgaatgtcac gatagctggt ttcatgatgc
     3481 tgcaccagat tgaccagcgg ctgcaggcta ctgtcaccca gccagcgttc catgcgtttg
     3541 aaccactgcg gcgtgtagta catttgtgac ggcacagtgc gaatgacgac caacgtttta
     3601 gcgccctgcc ttgccgcctc tttaaccgga atcgcatcac tgatcccgcc atccaggtag
     3661 ttaatgcctt ccagcgacac tccgctacga taaaagccag gtatcgcact ggaggcgcga
     3721 atcacatcca gccagttttg tttggttggt aaaaagtaat taggcgcgta gtcatcctga
     3781 cgacaggcgc acatataaaa cgatttgccg ctgtcaaaca accgcgcggc ggtatccatt
     3841 tgcaacggca tctggcttgc ggtggcctcc accagccagt cgagatcgat aagatttcct
     3901 ccacggacaa agcgcaatgg atcgaaaaat tcgcgttttg tggtatagcg catgatgact
     3961 ttacgcgcgt aaccgggctg attgcagata aacgccgaga ggttctgcgc cccggcagat
     4021 gtgccgagat aaagatcgaa aggattaaac tgcgcgcgca taaactcatc cagcacgcca
     4081 gccgtgaaaa ttccacgctg tccgccgcct tcgcacacca gagctattcg tccaggctgg
     4141 aacggcctta gcgacaacgg cgcaatatta ccaagcgtta caggtattcg ctgccccacc
     4201 tctgccttcc tgtttttgtt gtgaaagaat gacaaagtaa cgcaatttat tattcgctaa
     4261 aaccgcaaaa gccagtccgc tggactggct ttggataaaa ttttcttaat ggtctgaaat
     4321 ctagggtcgt tttcggccca tgaacaaact caccaggaac agaataatcc cgacgacaaa
     4381 gacaatttta gctgcgcctg cagcggtacc ggccaaacca ccaaacccaa gtgcggcggc
     4441 gattaacgcg ataaccagaa atatgatgcc ccaacgaaac ataagcgtct cctttaccat
     4501 agttaatgtc accgcttgat atgagcgtga aaatcactca atgcgatatt tttagtgtgg
     4561 tgcacatcgc gcctcccgac aaagttcggg aggacgaatt acgacgaatt acttagtttt
     4621 cagatcattt ttaacgcttt tcacaccatc taccgctttg gcgatacttt cagcacggtc
     4681 actttgtgcc tgagaatcta cggtaccgga gagctgaacc acgccgtcgg tggtttcaac
     4741 tttcacatga cgggaaggga cgatatcgtc cgccagcagt ttggctttga tttcactggt
     4801 ggtggcggtg tcacccgcgt agcccttcac cgagccttct ttagcgtcgc gaacgtgcag
     4861 tttgtcgctg acagaggtca ccccttcaac gcctttcgcc actttcactg cctcttcggc
     4921 ctgagcctag ctttcaacga aaccgctcag ggtcacgact ttttgctcgg ttttgacaga
     4981 gatatcggtg ctcttgatgt tgtcatgatc caccagcgcc gccttcactt tcgcggtgat
     5041 ggcgctgtca tccatgaaat taccgacttt attcatagag ctatcgactt tttgccctgc
     5101 gctttcattg gtagtctgtg cgttgttttc cgcgtaggca gagccggtcg cgacggcaga
     5161 ggtcaacatt acagccagca gagttttcga aatcttcagt cttgtcatag tcatcgattt
     5221 attcctgtat gtttgctcgt aatttgagcc tggcaacacg aggttgcatt gctgaatagg
     5281 gagagacttc accctctaca gaagtcaatg gccgccatca caaaagcgat gggtgatgaa
     5341 taacgaccat tacagcctct gaatcagtta ttaatatcgg tagaatgaca atcgacggct
     5401 ttagatactg atatctacgc attgaacggt atttaacgcc gtcagaaatg tcattacttt
     5461 gttaaatata gatcacaatt ttgaaaccgc tcgtgatatc agcgaaaaca taagcaaaag
     5521 tgaatgtttt aagaacattc cgtaagcggc taataaggaa gggaaattga cagggcgcag
     5581 cggctaccgc gccctggagg caagaattaa tgctcgcggg tctggtggaa ctgaacgtcg
     5641 ggataacgtt cctgcgccag gcgcaggttg accatgctgg tagcgatgta agcgaggtta
     5701 tcgccgccat caagcgccag ttggctttcg ttcttacgct tgaactcttc gaatttcttc
     5761 gcgtccgcac attctaccca gcgggcagtg gcaacgttga ctgattcata tactgcttca
     5821 acgttgtatt cgctcttcag gcgcgatacc accacatcaa actgcagcac accaacagca
     5881 ccaacgatca aatcgttgtt ggagatcgga cggaacacct gcaccgcgcc ctcttcggaa
     5941 agctgtacca gccctttgag cagctgtttt tgcttcagcg gatctttcag gcggatacga
     6001 cggaacagtt ctggtgcgaa gttcggaata ccggtgaact tcatcatctc accctgggta
     6061 aaggtgtcgc cgatctgaat agtgccgtgg ttgtgcaggc cgaggatatc gcccggatac
     6121 gcttcttcaa cgtgcgaacg gtcgcccgcc ataaaggtca gcgcgtcgga gataaccaca
     6181 tctttcgcag tacgcacctg gcgcagcttc atgccttttt catatttacc ggatactacg
     6241 cgcataaacg ccacgcggtc gcgatgtttc gggtccatat tggcctgaat tttaaatacg
     6301 aagccggtaa atttgtcttc gctcgcctct acggtacggg tatcagtctg acgcggcatc
     6361 ggcgcagggg cccactccac caggccatcc agcatatgat caacgccgaa gttacccagc
     6421 gcagtaccga agaataccgg agtgatttcg cccgcaagga acagttcttt gtcgaactcg
     6481 ttagacgcgc ctttcaccag ttccagttcg tcacgtagct gctgtgccag atcttcacca
     6541 accgcagcgt cgagatccgg gttattcagc cctttaacaa tacggacttc ctgaatcgtg
     6601 tggcctttac cgctctgata aagataggtt tcgtctttat aaaggtggta aacgccttta
     6661 aacagcttgc cgcagccaat cggccaggtg atcggtgcgc agccaatttt cagctcgttc
     6721 tcaacttcat cgagcagttc catcgggtcg cggatatcac ggtcaagttt gttcataaag
     6781 gtgaggatcg gcgtgtcgcg cagacgggta acttccatca gcttacgggt acgatcttca
     6841 acaccttttg cagcgtcgat aaccatcagg cagcagtcca ccgctgtcag ggtacgatag
     6901 gtatcttccg agaagtcttc gtgccccggg gtgtctagca ggttaaccag gcaatcgtga
     6961 tacggaaact gcatcacaga cgtagtaatg gagatcccac gctgcttttc catctccatc
     7021 cagtccgact tagcgtgctg gttggaacca cggcctttta ctgtaccagc ggtctgaatg
     7081 gcctgtccga acagcagcac cttctcggtg atggtagttt taccggcgtc cgggtgagaa
     7141 ataatggcaa aagtgcggcg cttcgccacc tcttgcaaat aaggagacaa cgtcataatt
     7201 ctttcttctt gagtaagcgg catcgcgccg cgcttgttga aaacgaaaaa ttgcggctat
     7261 tttacccatc aacggggggg aggcaatcag tgtttacaca ggagctgctc cagttcgtgc
     7321 aacgatgaaa cggtccaggt gggcgcgatg ccttctggtt gctcgagatg gtgcgcattc
     7381 agccagcaag tcgcaagccc ggcgttgatg ccaccgagaa tatcggactc ggcagtgtcg
     7441 ccaaccatca gcacgcgtga acggtcagga ttgcccgcca gttccagcgc ataatcgaaa
     7501 attttcttat tcggtttggc aatgccaact tcttcggaaa tcaccagcaa atcaaagtaa
     7561 tcacgcaggc ccgtccgttc cagacgcact tgctgcaagg cgctaaaacc gttggtgatg
     7621 atgccgattt tagcgttgcc acgaatggcg ttaagcagag aaaccgcgcc cggcagcggc
     7681 gtgcagattt ccgccatcgc attaataaag gcatcgttaa gcaagcctgc ttcaactttc
     7741 agccgttcgg cccagctctc gaaacgcccg tgctgaagct gtaatgaagt gatcgcgccg
     7801 ttttgataat ccacccacag tggcttgtta acggcctgat agtcctgaaa atcttcagcg
     7861 gtaaaggtga cgctgtaatc aagaaacatc cgctgcaggc cggtgaatga gtcaaaggta
     7921 aacagcgttt catcggcatc aaagaaaatc cagtcccact tcattattcc accttgtctt
     7981 acatactgat tggcaacgcc atgatgatgg cgtcttcgcg accgtccgtg gtggggtagt
     8041 aattgcggcg aatcgtcgcc tcgttaaagc ctaaactttc gtacagggca atggcggcag
     8101 cgtttgaagc acggacttcc agccatagtg tcgccacgcc gcgtttttcc agttcgtcga
     8161 tcagatgttt cagcagcacc cttcccaatc cctgacgctg atagtcagga tcgaccacaa
     8221 tattgaacaa tgtagcttca tccagcacca cttgcgtaat agcaaacgcc gccattttgc
     8281 cgttttgcgt taactgaaag ttgagataac gctcgccctg gttgctggca aagttttttt
     8341 cactccacgg aaaggcgtgg gcgcgttgtt caatgtggta agccgctggt aaatcagtcg
     8401 tttcgaggga agaaatcgtg ttcatatgtg caaatctgtt gccataacgc ggcgcgtgcc
     8461 gttgggtttg cccgtaattc ggtgagcgcc ggtgatgcca cctgagcgcc ttccagtgat
     8521 agcggttcgt cagtacccaa ccgccagctg ttgcagcgac tgccttgcgg cagcatcgcg
     8581 attttttctg gcgtcagttg cagcacttga tcggggctga cggttaatgc gcgcagaaca
     8641 tcgctcacta aaggatcggt cagggcggga agatcgtttg ccaccatcac cagacggacg
     8701 tgtgccggga tcgcaatggc aatctccccc tgcaacgcgc cagggcgacg cagcgaccac
     8761 tgggtaatgc ccagttgctg taactgccag tctcgtcggg atgtcatagc gaatcgctcc
     8821 tgttgtcagg ggcgcaaata tagcaaattc gtcgataccg cgccaacata tggctataat
     8881 cgccgccagt atcaattgag gagcattcca tgtctgcatt taccccggca agtgaagtct
     8941 tgctgcgtca cagtgatgat ttcgaacaaa gccgtattct gtttgccgga gacttacagg
     9001 atgacctgcc cgcgcgttta gataccgcgg ccagccgtgc tcatacccag caattccacc
     9061 actggcaggt attaagccgc cagatggggg ataacgcccg tttcagtctg gtcgccacgg
     9121 tggatgacgt cgcagattgc gatacgctga tttactactg gccgaagaac aaaccggaag
     9181 cccagttcca gttgatgaat ttactttctc tgctgccagt ggggacagat atttttgtcg
     9241 ttggcgagaa ccgcagcggc gtgcgcagcg ccgagcagat gctggcagat tatgcgccgt
     9301 tgaataaagt cgacagcgct cgtcgctgtg gcctctattt tggtcgtctg gaaaaacagc
     9361 cggtatttga tgccgataaa ttctggggcg aatacagcgt cgatggcctg acagtcaaaa
     9421 cgctgcctgg cgtgtttagc cgcgacggtc tggatgtcgg tagccagttg ctgctctcga
     9481 cgttaactcc gcacacgaaa ggtaaagtgc tggatgtcgg ctgtggcgcg ggggtgcttt
     9541 cagttgcctt tgcgcgccat tcgccgaaaa ttcgtctcac cttgtgcgat gtctctgcgc
     9601 cagcggtaga agccagccgc gcaacacttg cggccaactg tgttgaaggt gaagtctttg
     9661 ccagcaacgt cttttccgag gtgaaaggtc gttttgatat gatcatctcc aacccgccgt
     9721 tccacgatgg gatgcaaacc agcctggatg cggcgcaaac gctgattcgc ggcgcggtgc
     9781 gtcatcttaa tagtggcggc gagctgcgaa ttgtagcgaa cgccttcctg ccttatccgg
     9841 acgtgctgga tgaaacattt ggcttccatg aagtgattgc gcaaaccggg cgtttcaagg
     9901 tatatcgcgc cattatgacc cgccaggcga aaaaaggtta agtatcccgc cgcaagcctt
     9961 gccggatgcg gcgtaaacgc cttatccgac ctacaaaacc tgcacgttag cccttcgtag
    10021 gccagataag acgcgccagc gtcgcatctg gcatctgccc ccgcatgcct accaaacctg
    10081 cacgctagtt tcctgatgga catttttcca gcaattacac ctccggcgat aattaactat
    10141 tgacgaaaag ctgaaaacca ctagaatgcg cctccgtggt agcaattctt tttaagaatt
    10201 gatggtatgc gaaggtggcg gaattggtag acgcgctagc ttcaggtgtt agtgtcctta
    10261 cggacgtggg ggttcaagtc ccccccctcg caccaaaaac cacgttgata ttgctcgcac
    10321 tgggcgaagg tggcggaatt ggtagacgcg ctagcttcag gtgttagtgt tcttacggac
    10381 gtgggggttc aagtcccccc cctcgcacca aacgaggcga tatcaaaaaa aagtaagatg
    10441 attgtgcgaa ggtggcggaa ttggtagacg cgctagcttc aggtgttagt gtccttacgg
    10501 acgtgggggt tcaagtcccc cccctcgcac caattatctt tacttccttt cttgtttctt
    10561 ccttgatttt tcatccctga ttcaaattca ttatcatcag catgatggca gcaataagcg
    10621 ccaggcacga tggtagcaaa acgtaatgcc gtaactgttt gtgatacagc atcaccggcg
    10681 tcaatactaa tcccatcaga atgacaatcc gccactgatc cggcgataaa tccgcataca
    10741 tcagccctgc aatcagcaat cccggcagca acactcccca gccactgccc agcgtacgtt
    10801 gcaacatggt ttcatctctt tcattgataa tgataactaa tatcatatga taatttttat
    10861 catttgcaag ccagagaaat cccttgctat cgggtaaacc tatcgctagg attagcaatc
    10921 attatcattt agattactat cccgattatg gcctatcgtt ccgcaccgct ctatgaagat
    10981 atcatctggc gaacgcatct ccagccgcag gatgctgggc ttgcacaagc cgtacgtgcg
    11041 acgatcgcca aacatcgtga acatttgctc gagtttatcc gcctggatga acccgctccg
    11101 cttaacgcca tgacgctggc gcaatggtca tcaccgaatg cgctaagttc tctactcgcg
    11161 gtctattccg atcatatcta tcgcaaccaa ccaacgatga tccgcgagaa caaaccgctg
    11221 atctcactat gggcacaatg gtatatcggc ctgatggtgc caccattaat gctggcgcta
    11281 ctgacgcagg aaaaggcatt agatgtgtcg ccggaacatt tccatgctga gtttcacgaa
    11341 accggacgcg tcgcctgttt ctgggtcgat gtgtgtgaag ataaaaacgc aacaccacat
    11401 tcgccgcagc agcgaatgga aacgttaatc agccaggctc tggttccggt tgtgcaggca
    11461 ctagaagcga ccggagagat caacggcaaa cttatctgga gtaataccgg ttatttgatc
    11521 aactggtatc tcactgagat gaagcaactg ctcggcgagg cgacggttga atcgctgcgc
    11581 catgccctct tttttgagaa aacgctcacg aacggtgaag ataatccact ctggcgtacc
    11641 gtcgtgctgc gcgatggcct gctggtgcgc cgaacctgct gccagcgtta tcgcttaccg
    11701 gatgtgcagc aatgtggcga ttgtacgctg aaatgaaagc actgccgggg aagtaaaccc
    11761 ggcatcatgc ggattaatag ggatgcaaca cattacttgc ttcgcaatgc cgcatacata
    11821 acagaatatc tgcggcctcc aacacccctg catcagaact gacgcccagt ttcgacatca
    11881 cattaaattt atgtgcacgg atcgttttga tattgcgttt aagctgctcg gcaatttgtg
    11941 tcattgagta gccacgcgac ataaagcgca atatttctct ctccgtcggg cttagcgtcc
    12001 ggctttggtt aatgtaccac tgattgttca gtcggtcggt cgcctgacgt acaccattta
    12061 atgacaaaaa gagttcctga tgaaaaatct ccagcgttga cgctttactt aatacaccgt
    12121 ccagcggtga tggcgacaat gaaccaatca gccgagcttc aatatcatca tccgcaataa
    12181 ctaaacgccg ggtacgcgga aacttaatcg ccagttcagt caggcaagat aatccctctc
    12241 tgcgctcact tctcatggca gaaaaagaaa aatgaccgcc gcaaatgaga tccgcgacat
    12301 ggcatcctga aatgactcct gactggtata ggtatggagc ttataagggt tacccgcaaa
    12361 ctttctgaat aaactctcaa taccgatact gctcatgacg catttttcta tgagtgcgac
    12421 atttctcttc ttaattcggc tgtgttccat tctgcggcat ctccataaac agaaggcaag
    12481 ttaagttctc ttgcaccctg cgcgctattt atccatgcat acatttcagc gttgctgtga
    12541 attgccagtc gacgcatcgc actgtttttt tgggcgctga tagttttatt acttttctta
    12601 agcaacgaaa ctatctggtt gatgccccat ccttcaccta atagccgcag taccttgcgc
    12661 tctgaaagag tgagaatgac ggagcgatag ctaaaatcat gagtttcatt cattgccgga
    12721 gaggtcagca tggcgctgat acgctctgcg tgcgtattgc cggaacgtac ggtcttgacc
    12781 agattctcaa tgggttcaac atcagacaat aacgtcgccg tagggcacat gagcaattct
    12841 actgcctggg aataccagga gcgtgacacc atgaaaaccc agtgaatttc ccgatattgt
    12901 gagattaaag aataataatg ttcacaaaca gaacgtggat cttcactttc accggctaaa
    12961 tcagcaatga ctaatccgga acgacgtaat tgtaataagg taaagtcctc cgcagaggcg
    13021 ctggatatta tttcatattc agggaagtga gtcctcatga cctcttttaa ccctgcttgc
    13081 ataacaggta ttttactgat aacaattcca tttttgcagc atcctggcaa catatttcct
    13141 ccacatccac tgtctaaaaa tcatgatggt ttattcagga tattcattcc ttaaatgttt
    13201 ccagcaaatt aatagattaa gcgaataaat tatccttagc attcactttc atatcagcat
    13261 actcctgctg cacaacaact ccactgcatg tgtcagaagg ttagcatcaa atgataacct
    13321 ggtgaaatag cctggagaca tttaaaatat ctacattaag ttgttaggaa tacttaatga
    13381 aaaacagaaa tcacattcat ttaatttata attaaatgat atcccttccc gcagaatgaa
    13441 aagtcacaca accaatattt attcaatgaa atctgatggt tttaacttgt taacattaaa
    13501 aaacgtgccc ggttatctat tatcctgatt cgtaaaggcc aaacaataaa ttcattatag
    13561 tgataatatt catgtgatag tttgtttgcg gcgagagata attcgctttt tatcaccgag
    13621 cttgtcccga taaccaggat taatcagcgc aattttgatc ttaacctacc gcaaaacaac
    13681 cgtgcttcat gatacaaatg gattctcatt atctgaacaa tacgcagcac gtctatgaca
    13741 aagggcgagt tatgcaaact gagcaacagc gagccgtaac acggctttgt atccagtgtg
    13801 gattatttct tttgcaacat ggtgcggaaa gcgcgttggt tgatgagctt tcctcacgac
    13861 tgggtcgggc gttggggatg gacagcgtcg aaagttctat ctcttcgaac gccatagtgc
    13921 tgacaaccat taaagatggg caatgcctga catcgacacg taaaaatcac gatcgcggca
    13981 ttaatatgca tgtggtgact gaagtccagc acattgtgat tctggcggag caccatctgc
    14041 tggattacaa aggcgtagag aaacgattta gccaaattca gccattacgt tacccaagat
    14101 ggctggtagc cttaatggtt ggcctttctt gcgcctgttt ctgcaaactc aataaaggtg
    14161 gctgggatgg tgccatcatc accttctttg ccagtacggc cgcgatgtat atccgccagc
    14221 tgctggcaca acgtcatctt catccgcaga tcaacttttg cctcaccgct ttcgccgcca
    14281 ccaccatttc cggattgctt ttgcaacttc ccactttcag caatacaccc actatcgcga
    14341 tggccgccag cgttctgctg ctggtgccgg gatttccgtt gattaatgcc gtcgccgata
    14401 tgtttaaagg ccacatcaat accggactgg cacgctgggc gatcgccagt ctgctgacac
    14461 tggctacctg cgtcggcgtg gtgatggcac tgacgatttg ggggctacgc ggatgggtgt
    14521 gatcgagttt ctgtttgcat tggcgcagga tatgatcctc gccgccattc cagcggtcgg
    14581 ctttgcgatg gtgttcaacg ttcccgttcg ggcattacgc tggtgtgcgc tgcttggcgc
    14641 gattggtcat ggttcacgaa tgatcttaat gaccagaggg ctgaatattg agtggtcaac
    14701 ctttatggcc tccatgctgg tcggcaccat tggtattcaa tggtcgcgct ggtatctggc
    14761 gcatccgaaa gtgtttaccg tggcggctgt tatccctatg ttcccgggca tatcggctta
    14821 taccgcaatg atttcggcgg taaaaatcag ccagttaggt tacagcgaac cgttgatgat
    14881 taccctgtta accaactttc ttacagcttc atcgattgtt ggtgcgttat ccatcggtct
    14941 ttccattcct ggattatggt tgtaccgcaa gcgccctcgc gtataaaatt gcctcgctta
    15001 aagcacacgg atgagagaca gcctcctctc ctccgtgtgt tactataaaa gttatctccc
    15061 ttctcgttca tcgttccata tttgagaaac agtatgtctt ccagagtttt gaccccggac
    15121 gtcgttggta ttgacgccct ggtacacgat caccaaaccg ttctggcaaa agctgaaggc
    15181 ggtgtggttg ccgtatttgc taacaatgcc ccggcgtttt atgccgtcac gcctgcacgc
    15241 ctggctgaac tgctggcgct ggaagaaaag ctggcgcgtc cgggaagcga tgtcgctctg
    15301 gacgatcaac tctatcagga accgcaagcc gctcccgttg ctgtacccat ggggaaattc
    15361 gccatgtatc cggactggca acccgatgcc gattttatcc gcctggcggc gctatggggt
    15421 gtggcactaa gagagccggt gaccaccgaa gaactggcct cattcattgc ctactggcag
    15481 gcggaaggta aagtctttca ccatgtgcag tggcaacaaa aactggcgcg cagcctgcaa
    15541 atcggtcgtg ccagcaacgg cggactgccg aaacgagatg tgaatacggt cagcgaacct
    15601 gacagtcaaa ttccaccagg attcagaggg taacgatgaa aaacgttggc gacctgatgc
    15661 aacgcctgca aaaaatgatg cctgcccata tcaaacctgc attcaaaacg ggtgaagaac
    15721 tactggcctg gcagaaagaa caaggagcga tccgctccgc cgctctcgaa cgtgaaaatc
    15781 gggcgatgaa aatgcagcgc acctttaacc gctccggtat tcggccactg catcagaact
    15841 gctcctttga gaactatcgc gttgagtgtg aagggcagat gaatgcgtta agcaaagcgc
    15901 gccagtacgt cgaagagttt gacggcaaca tcgccagctt catcttttct ggtaagccag
    15961 gaaccggcaa aaaccatctg gcggcggcaa tctgcaacga gctgctgcta cgcggtaaat
    16021 ccgtattgat catcaccgtg gccgatatta tgtcggcgat gaaagatacc ttcaggaata
    16081 gcggtaccag tgaagaacaa ctgcttaacg atctgagcaa cgttgatctg ctggtgatcg
    16141 atgagatcgg cgtgcagacc gaatcgaaat acgaaaaagt gatcatcaac cagatcgtcg
    16201 atcgccgctc ttcttccaaa cgcccaaccg ggatgctgac caacagtaat atggaagaga
    16261 tgaccaagtt gctgggcgaa cgcgtgatgg accgtatgcg cctgggtaac agtttgtggg
    16321 tgatcttcaa ctgggatagc taccgtagcc gggtaacagg taaagagtat taatccccgt
    16381 ttctggcggt atactgcgac acaattccac cttgagtaat gatgatggaa actgttaaac
    16441 atcttctgtg ctgtgccatt gccgccagcg cattaatttc caccggggtg catgctgcgt
    16501 cctggaaaga tgcgctctcc agcgccgcca gcgaacttgg caaccaaaac agcacgacac
    16561 aggaaggcgg ttggtcgctc gcgtcattaa ctaacttgct tagcagcggg aaccaggcct
    16621 taagcgcaga taacatgaac aacgccgcag gcattctgca atactgcgcg aagcaaaagc
    16681 tggcgtcggt aaccgatgcc gaaaacatca agaaccaggt gctggaaaag ctgggcctga
    16741 acagtgaaga gcaaaaagaa gacaccaact atctggatgg cattcagggt ttgctgaaaa
    16801 ccaaagatgg tcagcaactc aatctggata acatcggaac gactccgctg gcagaaaagg
    16861 tgaaaaccaa agcctgcgat ctggtgttaa agcaggggct gaacttcatt tcctgaaccg
    16921 cgaaacgccg cgttttagct gttacccggc tgacgcggct ctctcctgcc cttttccctg
    16981 cttctggacg gttttccccc tgttacacgg gaagcgaccg cgatcaaaaa agtctgttta
    17041 taaactaatt cctaacaaca tcgcattttt ttgacactag aattgcagca atatggcgac
    17101 gccattacat tgttcttctc aaaaagctta aacgttggct aaacggcccg gtttagtgag
    17161 gatcgcccgt tgtcagaact actctctttc gccctttttc tcgcctctgt gctgatttac
    17221 gcatggaaag cgggacgtaa cacctggtgg tttgcagcca cattaacggt gctggggcta
    17281 tttgtcgttt taaatatcac cctgtttgcc agcgactatt ttactggcga tggtattaac
    17341 gacgcggttc tctatacctt aaccaacagc ctgaccggtg ctggcgtcag caaatacatc
    17401 ctgccgggta tcggcattgt gctggggctg acagcggtgt tcggtgcgct gggctggatc
    17461 ctgcgccatc gtcgccatca tccgcaccat tttggttaca gcctgctggc gctcttactg
    17521 gcgctgggtt cggtggacgc cagcccggca tttcgtcaga taacggaact ggtgaaatcc
    17581 cagtcacgcg atggcgaccc ggactttgcg gcttattata aagagccgtc gaaaactatc
    17641 cctgacccga aactcaacct ggtttatatc tacggcgaaa gtctcgagcg gacctatttt
    17701 gataacgaag ctttcccgga tctcacgcct gaactgggcg cgttgaaaaa tgaaggcctg
    17761 gatttcagcc acacgcagca gctgccagga acggattaca cgattgcggg catggtggct
    17821 tctcagtgcg gcataccgct gtttgccccc tttgaaggca acgcctccgc ctctgtctcc
    17881 agcttcttcc cgcagaacat ctgtctgggc gatatcctga aaaactcggg ttatcagaac
    17941 tatttcgtgc agggcgcgaa tctgcgtttt gccggtaaag atgtgttcct gaagtcgcat
    18001 ggcttcgacc acttatacgg ctcagaagag ctgaaaagcg tggtggccga cccgcactat
    18061 cgcaacgact ggggattcta cgacgatacc gttctcgatg aagcgtggaa aaagtttgaa
    18121 gagctttccc gctcaggtca gcgattctca ctgtttaccc tgacagtcga tacccatcac
    18181 ccggatggtt ttatctcgcg tacctgtaac cgcaaaaaat atgattttga cggtaagccg
    18241 aaccagtcat tcagcgcggt aagttgcagc caggagaata tcgcggcgtt tatcaacaaa
    18301 atcaaagcgt caccgtggtt taaagatacc gtcatcgtcg tctcttctga ccatttagcg
    18361 atgaacaaca cggcgtggaa atacctcaat aagcaggatc gcaataacct gttttttgtc
    18421 attcgtggcg acaagccgca gcaagagacg ctggcagtga agcgtaacac gatggataac
    18481 ggcgcgacgg tgctggacat tctcggtggc gataactatc tcggacttgg tcgtagcagt
    18541 ttatccgggc agtcgatgtc ggaaatcttc ctcaatatca aagagaaaac attggcgtgg
    18601 aagccggata tcatccgcct gtggaaattc cctaaagaga tgaaagagtt caccatcgac
    18661 cagcagaaaa acatgattgc cttctcgggt agccatttcc gtttgccgct gctgttgcgg
    18721 gtttcagaca aacgcgtgga accgctgccg gaaagcgaat actcagcacc gctgcgtttc
    18781 cagctggccg atttcgctcc acgcgacaat ttcgtctggg ttgaccgttg ctataagatg
    18841 gcacaactct gggctccgga actggcactc tccaccgact ggtgtgtctc gcaagggcag
    18901 cttggcggtc agcaaattgt tcaacatgtt gacaaaacaa catggcaggg caaaacggca
    18961 tttaaagata cggtcatcga catggcgcgt tataaaggca atgtcgatac gctgaaaatt
    19021 gttgataacg atattcgcta caaagccgac agtttcatct tcaacgtcgc cggtgcgcca
    19081 gaagaggtga aacagtttag tgggatttcc cgtccagagt cgtggggccg ctggtccaac
    19141 gcgcagctgg gcgatgaagt aaaaatcgag tacaagcatc cgctgccaaa gaaatttgac
    19201 ctggtgatta ccgccaaagc atacggcaat aacgccagcc gtcctattcc ggtacgcgta
    19261 ggcaatgaag aacaaaccct tgtgctgggc aatgaagtga ccaccaccac actgcatttc
    19321 gataacccaa ccgatgccga cacactggta attgtgccgc cggaacctgt ctcaaccaac
    19381 gaagggaata tcctcggaca ctcgccacgt aagctcggga tcggcatggt ggaaattaaa
    19441 gtggtggaac gtgaaggata attcctgact gatgtgtagg tcgcataagg tgcgacctac
    19501 acaccttctg cggcctacaa aacatcattg agaaacagaa aaaaacagcg cccaacactt
    19561 ccccggacgc tgcatggcaa ctttaataat ctcgccttat cagaaagtaa ttacaccctt
    19621 aatcaactca cgattgttaa tcacatcgcg ctcgtaggtt tctgccagcg tggcgaacgg
    19681 atagcgatgg gttaacatca tgtcagcagt gatttttcct tccgccatca gtcgaccgac
    19741 tttagcaaaa tcttccggcg tggcgttgcg gctacccatc atcgtcgttt cttttttatg
    19801 gaattccgga tcggagaact gcaactcacc tttaaacagg ccgacaaata ccaccgtgcc
    19861 gccgtgacga atcaaattca cggtgttatt catcgcatgt tgattacctg tcgcgtcgat
    19921 cactttctgc gccagcgaac caccaaactg cgcccgtagc tgcgcgtcaa aatcttcggc
    19981 tgacgggtcc agtaaaggta attccagacg cgttgccaca tgttcacggc gcgccggact
    20041 ggtatccgcc accaccacct gtgcgccatc ggctttagcg attgccgccg cgcccagacc
    20101 gattggcccc gccccgacca ccagcacctg ctcgccggga gcaatggctg cgcgacgcac
    20161 cgcatgagcg ctaatagcga aaggttcaat caatgctgcc gcctgcgggt caataccgtc
    20221 tgcgggcaaa atgttcgcca ccggcacgct caaatactca ctaaaaccgc catcctgatg
    20281 cacgccaatg actgaaattt tttcacagca attggtacgc ccgcttttac acgccgggca
    20341 ttgctgacag gcaacataag ggatcacagc aacttgcaga ccatttttaa gatcagcaat
    20401 atttttaccc agcctaacaa tctccccaca tatttcatgg cctaaaacac gtggataact
    20461 aaaaaatggt tgatttccac cccaggcatg aatatcggta ccgcaaatcc cgacagactt
    20521 aatttttatt aatgcttcat tgtcacccgg aataggtatc tcgcgttgtt tccagactaa
    20581 ttctttcggc tgctggcaaa ttaaaacatt catcgtagac atgattcggc tctccatttt
    20641 ttgttgcctt agatatcaat gaaaatgaaa aatggcagcg gaacggaaaa tctgttttgt
    20701 gaaaacacac gcataaaaat gttttaaatc gggttttaat ggactcacaa ggaggaaaat
    20761 atgagtcgtt cacaaaattt acgccacaat gtgattaacc aggtcattga tgatatggcg
    20821 cggggtcata tcccttcccc acttccatcg caaagtgcgc tggcggaaat gtacaacatc
    20881 agccgcacca cagtgcgtca tattctcagc catttacgcg aatgcggcgt cttgacgcag
    20941 gttggaaacg actatgttat tgcgcgtaaa cctgaccacg atgacggttt cgcctgtact
    21001 accgcttcaa tgagtgaaca aaacaaagtg tttgagcagg cttttttcac gctgataaac
    21061 cagcgccagt tacgcccagg ggaaactttt tctgaactgc aactggctcg ggccgcgggt
    21121 gtcagcccgg tggttgtaag agaatatctt ttaaaattcg gccgttataa tcttattcaa
    21181 agcgaaaagc gcggccagtg gagcatgaag caattcgatc aatcctacgc cgagcagctg
    21241 tttgagctac gcgaaatgct cgaaacacat tcacttcagc actttctcaa cctgcctgat
    21301 catgatcccc gctggctgca ggcgaaaacc atgctcgaac gtcatcggct actgcgtgac
    21361 aacatcggca atagctttcg catgttctcg caacttgaca gagattttca ctccttactg
    21421 ctttcagctg ccgacaatat cttttttgat caatcgcttg agatcatctc agtcatcttt
    21481 cattttcact atcaatggga cgaaagcgat cttaaacagc gcaatatcat tgccgtcgat
    21541 gagcatatga ccattctcag cgctctgatt tgccgcagtg acctcgatgc cacccttgcg
    21601 ctccgtaacc atttgaattc agctaaacaa tcaatgattc gatccattaa tgagaacacg
    21661 cgttatgctc attaagtacg gattaaaaac agcaaactat tagcgggtgc acggttttat
    21721 caaaagataa ataccgggaa attcccgctt acctatgctc acaatcagtc gactaaaaat
    21781 gtttttgtga gcggtagtaa agtcctaaaa ctttaacctg ccgttaatgt caaccttgcg
    21841 taatgcacaa ttacgtaaac aattaaaatt ataaatacag gagtccggcg tggaaaaaga
    21901 aaatatcacc atcgatccgc gttcttcatt tactccatct tcgtcggcag atattcccgt
    21961 gccaccagat ggattagttc aacgcagtac ccgaattaaa cgcattcaaa ccaccgccat
    22021 gttgttatta ttttttgcgg cggtaatcaa ttatctcgac cgcagttcgc tgtcggtagc
    22081 aaatttaacg attcgtgaag aattgggatt aagtgccact gaaatcggcg ctttgctctc
    22141 cgtgttttca ctcgcttacg ggattgcgca acttccttgc ggcccactat tggatcgtaa
    22201 aggcccacgc ctgatgctgg gactggggat gttcttctgg tcactgttcc aggcaatgtc
    22261 tggcatggtg cacaacttta cgcagttcgt gttggtgcgt atcggtatgg ggattggtga
    22321 agcgccgatg aacccatgcg gtgtaaaagt cattaacga
//
LOCUS       DAJVUJ010000060        20821 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.060, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000060 DAJVUJ010000000
VERSION     DAJVUJ010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20821
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.060"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(57..602)
                     /gene="hemG"
                     /locus_tag="OQQ48_002050"
     CDS             complement(57..602)
                     /gene="hemG"
                     /locus_tag="OQQ48_002050"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HCS1952828.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMSSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAQLTNKPTLK"
     gene            complement(614..2065)
                     /gene="trkH"
                     /locus_tag="OQQ48_002051"
     CDS             complement(614..2065)
                     /gene="trkH"
                     /locus_tag="OQQ48_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HCS1952829.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            complement(2104..2718)
                     /locus_tag="OQQ48_002052"
     CDS             complement(2104..2718)
                     /locus_tag="OQQ48_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HCS1952830.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGIGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            complement(2718..4049)
                     /gene="pepQ"
                     /locus_tag="OQQ48_002053"
     CDS             complement(2718..4049)
                     /gene="pepQ"
                     /locus_tag="OQQ48_002053"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HCS1952831.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHASVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHLQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFSGIRIEDNVVIHENNVENMTRDLKLA"
     gene            4239..6449
                     /gene="fadB"
                     /locus_tag="OQQ48_002054"
     CDS             4239..6449
                     /gene="fadB"
                     /locus_tag="OQQ48_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HCS1952832.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPRSSFLVPEEQLSQWLHFANSVF
                     NRLEDLPVPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVR
                     MPRMLGADSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAVLRQAINGDLDW
                     KAKRQPKLEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEA
                     LNLENKSFVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGG
                     IAYQSAWKGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTL
                     DYAGFDRVDVVVEAVVENPKVKKAVLAETEQKVRPDTVLASNTSTIPISELANALERP
                     ENFCGMHFFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNR
                     VLFPYFAGFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAG
                     FPQRMQKDYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQ
                     PKRDFSEEEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWL
                     NTLGSAKYLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            6459..7622
                     /gene="fadA"
                     /locus_tag="OQQ48_002055"
     CDS             6459..7622
                     /gene="fadA"
                     /locus_tag="OQQ48_002055"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HCS1952833.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCN
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(7814..8515)
                     /gene="fre"
                     /locus_tag="OQQ48_002056"
     CDS             complement(7814..8515)
                     /gene="fre"
                     /locus_tag="OQQ48_002056"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HCS1952834.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDYQIVVDIPYGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLSELEALSLKHP
                     GLRVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(8561..10054)
                     /gene="ubiD"
                     /locus_tag="OQQ48_002057"
     CDS             complement(8561..10054)
                     /gene="ubiD"
                     /locus_tag="OQQ48_002057"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HCS1952835.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGVPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRDGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            10221..10709
                     /gene="rfaH"
                     /locus_tag="OQQ48_002058"
     CDS             10221..10709
                     /gene="rfaH"
                     /locus_tag="OQQ48_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HCS1952836.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            complement(10706..11488)
                     /gene="tatD"
                     /locus_tag="OQQ48_002059"
     CDS             complement(10706..11488)
                     /gene="tatD"
                     /locus_tag="OQQ48_002059"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HCS1952837.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAVQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDFTPKPSS
                     RRNEPAHLHHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(11530..12306)
                     /gene="tatC"
                     /locus_tag="OQQ48_002060"
     CDS             complement(11530..12306)
                     /gene="tatC"
                     /locus_tag="OQQ48_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HCS1952838.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            complement(12309..12824)
                     /gene="tatB"
                     /locus_tag="OQQ48_002061"
     CDS             complement(12309..12824)
                     /gene="tatB"
                     /locus_tag="OQQ48_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HCS1952839.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELIQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            complement(12828..13097)
                     /gene="tatA"
                     /locus_tag="OQQ48_002062"
     CDS             complement(12828..13097)
                     /gene="tatA"
                     /locus_tag="OQQ48_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HCS1952840.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            complement(13176..14816)
                     /gene="ubiB"
                     /locus_tag="OQQ48_002063"
     CDS             complement(13176..14816)
                     /gene="ubiB"
                     /locus_tag="OQQ48_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HCS1952841.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            complement(14813..15418)
                     /gene="ubiJ"
                     /locus_tag="OQQ48_002064"
     CDS             complement(14813..15418)
                     /gene="ubiJ"
                     /locus_tag="OQQ48_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HCS1952842.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            complement(15432..16187)
                     /gene="ubiE"
                     /locus_tag="OQQ48_002065"
     CDS             complement(15432..16187)
                     /gene="ubiE"
                     /locus_tag="OQQ48_002065"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HCS1952843.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            complement(16282..17709)
                     /gene="rmuC"
                     /locus_tag="OQQ48_002066"
     CDS             complement(16282..17709)
                     /gene="rmuC"
                     /locus_tag="OQQ48_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HCS1952844.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            complement(17850..18611)
                     /gene="udp"
                     /locus_tag="OQQ48_002067"
     CDS             complement(17850..18611)
                     /gene="udp"
                     /locus_tag="OQQ48_002067"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HCS1952845.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            18873..19685
                     /locus_tag="OQQ48_002068"
     CDS             18873..19685
                     /locus_tag="OQQ48_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCS1952846.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGMHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKRS"
     gene            20079..20739
                     /gene="hcp"
                     /locus_tag="OQQ48_002069"
                     /pseudo
     CDS             20079..20739
                     /gene="hcp"
                     /locus_tag="OQQ48_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001680647.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
BASE COUNT         5097 a         5618 c         5219 g         4887 t
ORIGIN      
        1 ctgacaagag gaaatttaaa ataattttct gaccgcgcac tttttattct ttatgcttat
       61 ttcagcgtcg gtttgttggt taactgggcg atttctcggg cgaaattcgc cacctgctcc
      121 caatcggtat agacaacttc tttgcgcgta tccgtttcac cgcctgacat cttcataatc
      181 agcttaatca taaaacggtc gtaccagcga taacgtgggt aacgcagcgc cccggcaatg
      241 accgcgcagc gatcgggacg ccattgtgaa ctcatcagaa acttgcgcgc gtagctgttg
      301 gtctgtggag tacgcttctc cggtttgcgc gccaccaggt ttacggaata aaaggcgctc
      361 ggcatcgaat tcagtcgcgt cgcatgtttt ttgacaaatt cctggaacgc agagtggtag
      421 tgaccatagc gaatagaagc accaatgacc acacggtcat agttttccca ttgtggttct
      481 tcaatgcggt gcacattggc gacatccgtc tggatcccca gttctttcag ttccgaagcc
      541 aggtaggagg caatctcgcg cgtttgtccg tcccttgttg agaaaagaat taatgttttc
      601 acgtattact ccatcattca cgccagaaag tcggggtaaa gagcaccagc aatgtaaaga
      661 cctcgagacg accaaacagc atgttggcaa tcaggatcca tttagccacc gggttcatac
      721 tggtaaagtt atcagcaacc acgccaagcc ctggccccag gttattcaat gtcgcaacaa
      781 ctgaggcaaa ggcagaaaaa tcatccacgc ccgtggcgat aatcgccagc atactgacaa
      841 taaacaccaa tgcataggcg gagaaaaatc cccaaacggc ttcgaggata cgttccggca
      901 gtgcgcgatt ccccagctta atgctataga cggcgttcgg atgcaccagt cgtttcagct
      961 cacggttccc ctgcttaaac agcagcagga tgcggatcac tttcaggcca ccgcccgttg
     1021 acccggcgca accgccgata aaagctgaac ataaaagcag taccggcaaa aagagcggcc
     1081 agcgggcaat gctgtcagtc gtaaacccgg cggttgtcgc catcgatacc acctggaaaa
     1141 acgcctggtt aattgtcatc agcgccgaac tgtagacatt atgaaaccac agtacgagtg
     1201 tacaaataac caccagcgta aactgtacgc cgataaacat gcgaaattcc ggatcgcgcc
     1261 aataaacctt cagactacgc ccacttaaca gtgaaaagtg cagaccgtag ttacaaccgg
     1321 agatcagcag gaagatagca atgatagtgt taatagtcgg actgtcgaaa taaccgatac
     1381 tggcatcatg tgtcgagaag ccgccaatag cgatagtcgc aaagctatgg ccgatggcat
     1441 caaaggcatc cattccggca aaccacaacg ccagcgcaca ggcgacggtc agcaagacat
     1501 aaatcaacca cagggttttc gccgtttccg caattcgcgg gcgcattttg ttatctttca
     1561 gcgggccggg catttctgcg cgatagagct gcatcccacc cacgccgagg ataggcagta
     1621 tcgcaaccgc taacacgatg atccccatcc cgccaaacca ttgcagcatc tggcgataaa
     1681 agaggatggc atgagggagc gaatccagcc ccaccagcgt agtggccccg gtggtggtca
     1741 ggccagagaa agattcaaaa aacgcatcgg taatcgtgag gttcgggctt tccgagaaga
     1801 taaaagggag cgcaccgaca ctacccagca cggtccaaaa cagcaccact atcagaaacc
     1861 cctcacggga tttcagttcg cccttctctt tgcggttcgg ccaccacagc atagagccaa
     1921 tggcgagggc gacaaaaaag gtctgggtaa aagcgcggcc cgctccatcc cggtagatga
     1981 gtgctaccag cccagggata atcatggtcc ctgaaaataa gatgaccagt agtccaacga
     2041 ttcgggtaat ggcgcgaaaa tgcatctctg ccgcttcctt agttctgcaa aatgaagtgg
     2101 ggattattct tcaatcgcta acaattgcaa tgaaccacgg ctaaaatccg ccagctttgc
     2161 ggaaaattca gccactttcg ccgccggaag cgccacccgc agcagaacga atgcctgata
     2221 atcactgttg ataattttgc cgtcacactg ccccagcaac gcttcaatgc cggttaactg
     2281 actatattca cattgcaaag tatattcggt taatggcgtc ttgcgttggg tcgttagctg
     2341 gcgcagcgcc tgattcacgc cgccgccata cgctttcact aacccaccgg tgccaagcaa
     2401 tatgccgccg tagtagcgca ctaccacagc ggtaatttcc ccgatgccgc tgcccattag
     2461 ctgggcgagc atcggtttac ctgccgttcc cgccggctcc ccgtcgtcag agaaacccag
     2521 ctgttgagaa tcatccggcg cacccgccac ccacgccacg caatggtggc gggcatcggg
     2581 gtgttctgcc cgcaccgatt caacaaacgc tttcgccgcc tcaacgccat cggtatgcgc
     2641 caacatcgtt atgaagcggc tctttttgat ctcttcaacg accgtgaccg gtgccgcagg
     2701 aattaaccag ctttccatca cgccagtttc agatcccggg tcatgttttc cacgttattt
     2761 tcgtggatca ccacgttgtc ttcgatacga atgccgctga acggtttcag tgcttcaatt
     2821 ttctgccagt tgaagtgctt gctgaactgc ccttcacgcc acggtgccag tagcgattcg
     2881 atgaagtaga tacccggttc gatggttaac accatgcccg gctggagaat acgggtgcag
     2941 cgcaggtacg gatattttgc cggtgccgcg aggtgtgtac cgctatcatc ctgcataaaa
     3001 ccggctacgt catgcacctg caggcccagc ggatggccga taccgtgcgg cataaacggt
     3061 ccggtgagat cgttttcgac catcgcttct tcactcatat cggtgatgat ttgatgttta
     3121 cgcagcaatt tggcgatgcg ctgatggaac tggaggtggt aatccacata gctgacgcca
     3181 gctttcatgg tagcgatcag cgcaagttgt tcatcattta cgtctttcac cagctgtgcg
     3241 tagtcgttgt cgctttttgc cgaccaggta cgagtcagat cagccgcata gccgttatat
     3301 tcggccccgg catccagcag gaagctgcgc atctcttccg gtgcctgatg atccagtttg
     3361 gtgtaatgca gcaccgaagc gtgttcgtta agcgccacaa tgttgctgta aggtacgtcg
     3421 gtatcacgat gaccggtcgc ggtcagatag gcgatgttga tatcgaactc gctcatgcca
     3481 gaacggaacg cttcttctgc cgcacgatga ccgttgaccg ccattttctg cgcttcacgc
     3541 atacaggcca gttcgtactc ggttttgaag gagcggtagt aatgcaggta gtcgatcacc
     3601 cctttcgggt tgatgttgct ggcctcaata cccagttgca gcgcacgttc cggcaccgga
     3661 ccgatataac cgatattgcc gcgcgcagcg ggcagcaggc taccaatgcc atcggctttc
     3721 ggcagtgcga tcacttccac atcttcagtc cagaaggagg tcggcagcgg ttcgacgttg
     3781 tgccagtaat caaccggcag atagaaccac agtttcggct tgttcacgcc atccaccagc
     3841 aaccagcagt ttggcacctg agttaccggc acccacgctt tgaattgcgg gttcacttta
     3901 aacggatagg gatgatcgtc gagaaaaacg ttgaacagct cgccggagtg aataagtaat
     3961 gcatccagct tgaagcgcgc cagcgcatcg cgagtccgtt cttgtaaggt agctatatga
     4021 tttttataga gcgaggccag tgattccatt ttttaccctt ctgttttttt gaccttaagt
     4081 ctccgcatct tagcacatcg ttcatccaga gcgtgatttc tgccgagcgt gatcagatcg
     4141 gcatttcttt aatcttttat ttacatgttt ttaacacaaa atacacactt cgatgcatct
     4201 ggtacgacca gatcaccttg cggattcagg agactgacat gctttacaaa ggcgacaccc
     4261 tgtaccttga ctggctggaa gatggcattg ccgaactggt atttgatgcc ccaggttcag
     4321 ttaataaact cgacactgcg accgtcgcca gcctcggcga ggccatcggc gtgctggaac
     4381 agcaatcaga tctaaaaggg ctgctgctgc gttcgaacaa agcagccttt atcgtcggtg
     4441 ctgatatcac cgaatttttg tccctgttcc tcgttcctcg ttcctcgttc ctcgttcctg
     4501 aagaacagtt aagtcagtgg ctgcactttg ccaatagcgt gtttaatcgc ctggaagatc
     4561 tgccggtgcc gaccattgct gccgtcaacg gctatgcgct gggtggtggc tgcgaatgcg
     4621 tgctggcgac cgattatcgt ctggcgacgc cggatctgcg catcggtctg ccggaaacca
     4681 aactgggcat catgccgggc tttggcggtt ctgtacgtat gccacgtatg ctgggcgctg
     4741 acagtgcgct ggaaatcatt gccgccggta aagatgtcgg cgcggatcag gcgctgaaaa
     4801 tcggtctggt ggatggcgta gtcaaagcag aaaaactggt tgaaggcgca aaggcggttt
     4861 tacgccaggc cattaacggc gacctcgact ggaaagcaaa acgtcagccg aagctggaac
     4921 cactaaaact gagcaagatt gaagccacca tgagcttcac tatcgctaaa gggatggtcg
     4981 cacaaacggc ggggaaacat tatccggcgc ccatcaccgc agtaaaaacc attgaagctg
     5041 cggcccgttt tggtcgtgaa gaagcattaa atctggaaaa caaaagcttt gtcccgctgg
     5101 cgcacaccaa cgaagcccgc gcactggtcg gcattttcct taacgatcaa tatgtaaaag
     5161 gcaaagcgaa gaaactcacc aaagacgttg aaacaccgaa acaggccgct gtactgggcg
     5221 caggcatcat gggtggcggc attgcttacc agtctgcgtg gaaaggcgta ccggttgtca
     5281 tgaaagatat caacgacaag tcgttaaccc tcggcatgac cgaagccgcg aaactgctga
     5341 acaagcagct tgaacgcggc aagatcgatg gtctgaaact ggctggcgtg atctccacga
     5401 tccacccaac gctcgactac gccgggtttg accgcgtgga tgttgtggta gaagcggttg
     5461 tcgaaaaccc gaaagtgaaa aaagccgtac tggcagaaac cgaacaaaaa gtacgcccgg
     5521 ataccgtact ggcgtctaac acttcaacca ttcctatcag cgaactggcc aacgcgctgg
     5581 aacgcccgga aaacttctgc ggaatgcact tttttaaccc ggtacaccgg atgccgttgg
     5641 tagaaattat tcgcggcgag aaaagctccg acgaaaccat cgcgaaagtt gtcgcctggg
     5701 cgagcaagat gggcaagacg ccgattgtgg ttaacgactg ccccggcttc tttgttaacc
     5761 gcgtgctgtt cccgtatttc gccggtttca gccagctgct gcgcgacggc gcggatttcc
     5821 gcaagatcga caaagtgatg gaaaaacagt ttggctggcc tatgggtccg gcatatctgc
     5881 tggatgtcgt gggcattgat accgcgcatc acgctcaggc tgtcatggca gcaggcttcc
     5941 cgcagcggat gcagaaagat taccgcgatg ccatcgacgc gctgtttgat gccaaccgct
     6001 ttggtcagaa gaacggcctc ggtttctggc gttataaaga agacagcaaa ggtaagccga
     6061 agaaagaaga agacgccgcc gttgaagacc tgctggcaga agtgagccag ccgaagcgcg
     6121 atttcagcga agaagagatt atcgcccgca tgatgatccc gatggtcaac gaagtggtcc
     6181 gctgtctgga agagggcatt atcgccactc cggcggaagc ggatatggcg ctggtctacg
     6241 gcctgggctt ccctccgttc cacggcggcg cgttccgctg gctgaacacc ctcggtagcg
     6301 caaaatacct cgatatggca cagcaatatc agcacctcgg cccgctgtat gaagtgccgg
     6361 aaggtctgcg taataaagcg cgtcataacg aaccgtacta tcctccggtt gagccagccc
     6421 gtccggttgg cgacctgaaa acggcttaag gagtcacaat ggaacaggtt gtcattgtcg
     6481 atgcaattcg caccccgatg ggccgttcga agggcggtgc ttttcgtaac gtgcgtgcag
     6541 aagatctctc cgctcattta atgcgtagcc tgctggcgcg taacccggcg ctggaagcgg
     6601 cggccctcga cgatatttac tggggttgtg tgcagcagac gctggagcag ggttttaata
     6661 tcgcccgtaa cgcggcgctg ctggcagaag tgccgcactc tgtcccggcg gttaccgtca
     6721 atcgcttgtg tggttcatcc atgcaggcac tgcatgacgc agcacgaatg atcatgactg
     6781 gcgatgcgca ggcatgtctg gttggcggcg tggagcatat gggccatgtg ccaatgagtc
     6841 acggcgtcga ttttcacccc ggcctgagcc gcaatgtcgc caaagcggcg ggcatgatgg
     6901 gcttaacggc agaaatgctg gcgcgtatgc acggtatcag ccgtgaaatg caggatgcct
     6961 ttgccgcgcg gtcacacgcc cgcgcctggg ccgccacgca gtcgggcgca tttaaaaatg
     7021 aaatcatccc gaccggtggt cacgatgccg acggcgtcct gaagcagttt aattacgacg
     7081 aggtgattcg accggaaacc accgtggaag ccctcgccac gctgcgtccg gcgtttgatc
     7141 cagtaaacgg tacggtaacg gcgggcacat cttctgcact ttccgatggc gcagctgcca
     7201 tgctggtgat gagtgaaagc cgcgcccatg aattaggtct taagccgcgc gctcgtgtgc
     7261 gttcgatggc ggtcgttggt tgtaacccat cgattatggg ttacggcccg gttccggcct
     7321 cgaagctggc gctgaaaaaa gcggggcttt ctgccagcga tatcggcgtg tttgagatga
     7381 acgaagcctt tgccgcgcag atcctgccgt gcattaaaga tctcgggcta atggagcaga
     7441 ttgacgagaa gatcaacctc aatggtggtg cgatcgcgct gggtcatccg ctgggctgtt
     7501 ccggtgcgcg tatcagcacc acgctgctga atctgatgga acgcaaagac gttcagtttg
     7561 gtctggcgac gatgtgtatc ggtctgggtc agggtattgc gacggtgttt gagcgggttt
     7621 aaccacttgc cggatgcggc gtgaacgcct tatccggtct accgatcctg caccaatgta
     7681 ggcctgataa gacgcgccag cgtcgcatca ggcatcgtgc accgattgcc ggatgcgaca
     7741 tagatctaaa tgccactgaa taaagtttta gttgccgttc ttcccgcctg tcaggggcgg
     7801 gtttttttat atctcagata aatgcaaacg catcgccaaa cagacgatct tcccgcgcat
     7861 tacgctcact gcaaaacagg tcacgagcaa ttttcgccat ctcaaaacgt ccggcaatat
     7921 agatatcatg ctctgccagc gtaccatgat cctgcaagac cgccgttaac acggtgccag
     7981 tacgcccacg ccagcccgct tccggttgtt caaccaccgg caccacacgc aggccaggat
     8041 gcttcagcga aagcgcctca agctcggaga gatcataaag atgttgctcc tcacgcccac
     8101 cccagtaaat ggtgatatcg cgattgggat tacgcgccaa cgccgtcaat aaaatcgagc
     8161 gcgcatagga gaacccggtg ccgcccgcaa tcaaaatcat cggacgctct tcatcatcgc
     8221 gcagccacgc ttctccgtag ggaatatcga ccacgatttg ataatctttg aggatgcggt
     8281 ccatgactgc tttcgcgtaa aggttgattt cagaagcgcc aatatgcagc tctataaacc
     8341 ctttttcatc cggcgtcgaa gccattgaaa acggacgttt gtcgcgctca tccatcacta
     8401 ccatcaaata ctgaccagca cgaaaagaaa aggccgcgtc tggcacgatg cggacacgat
     8461 atacggtatc cgtgatagct tctaccgagg tcactttaca gcttaaggtt gtcatgcgct
     8521 ttctctgtcg gatcgataaa tagggcaaaa caaacgcgca tcaggcgctt ttaccgttgt
     8581 taaaaatagc cagttcatcc cagatggcgt caatatgcgc gacaacatct ggatcttttt
     8641 tgatgggacg tccccattca cgctgggttt cccccggcca tttattcgtg gcatccagcc
     8701 ccatttttga acccagcccg gagacaggcg aggcaaaatc cagataatca ataggcgtat
     8761 tttctaccag aacagtatcc cgcgccgggt ccatacgggt ggtaatcgcc caaatcacat
     8821 cgttccagtc gcgtgcgttg acgtcatcat cgcaaacgat cacaaattta gtgtacataa
     8881 actggcgtaa gaacgaccag acgcccatca tgacgcgctt cgcgtgtccg gcgtactgtt
     8941 ttttgatcgt cactaccgcc agacgataag aacagccttc cggcggcagg taaaaatcga
     9001 caatttccgg gaactgtttt tgcagaatcg gtacaaacac ttcgttcaac gccacgccca
     9061 gtaccgcggg ctcatctggc ggacgcccgg tataggtgga atggtaaatc gcatcttcac
     9121 gctgggtaat atgcgtcacg gtaaataccg ggaaactatc gacttcgtta tagtaaccgg
     9181 tgtggtcgcc atacggccct tccggcgcag tttcgccttg ttcgatatac ccttccagca
     9241 caatctccgc actggcgggc acttcaaggt cattggagat acacttcacc acttcggtct
     9301 tggtaccgcg cagcaatccg gcaaacgcat actctgaaag tgtatccgga acgggggtga
     9361 ctgcaccgag aatcgtggcg ggatcggcac ccagcgccac agaaaccggg aaacgttcgc
     9421 ccggatgcgc cgcacaccac tcctgataat ccagcgcgcc gtcgcgatgc gacagccagc
     9481 gcataatcag tttgttttta ccaatcagct gctggcgata aatgcccaga ttctgccgct
     9541 ctttatgcgg gccgcgcgtc actgtcagcc cccaggtaat cagcggtgcg gcatcttccg
     9601 gccagcaggt cataatggga atgcgattga gatcgacgtc atcgccagag acgatttttt
     9661 gttggcaggg cacaccacgc agccgctttg tcggcatgtt caatacttgc ttaaactgcg
     9721 gcagtttatc aaacaggtcg cggaaacctt ttggcggctc cggctctttc agaaacgcca
     9781 ataatttacc aacttcacgc agcgccgaaa catcttcctg tcccatgccc atcgccacgc
     9841 gctttggcgt accgaacagg ttgcacagca ccggcattga gtagccttta gggttttcga
     9901 acaacagcgc aggcccaccg gcacgcaaag tgcggtcagc aatttcagtg atttccagat
     9961 gcggatccac cgggagcgtg atacgtttta gctcaccctg ctgttcaagc agcgtcaaga
    10021 agtcgcgtaa atcgttatat ttcatggcgt ccattgtagc ctcttaatct acgcccatta
    10081 tacggcgttc atctttgcga tgctgtaaat ttgttaaatt agcgtgaact ctgacggtat
    10141 aacgcaaacc ggggaatata attaacttag cgtaaagctt ttgctatcct tgcgccccga
    10201 ttaaacggat aagagtcatt atgcaatcct ggtatttact gtactgcaag cgcgggcaac
    10261 ttcaacgtgc ccaggaacac ctcgaaagac aggctgtgaa ttgcctggca ccgatgatca
    10321 ccctggaaaa aatcgtgcgt ggaaaacgta ctgcagtcag tgagccattg tttcccaact
    10381 acctgtttgt cgaatttgat ccagaagtga ttcataccac gactatcaac gcgacccgcg
    10441 gtgtcagcca cttcgtgcgc tttggcgcgt cgccagcgat agtcccatcg gcggtgattc
    10501 atcagctatc ggtatataaa ccgaaagaca ttgtcgatcc ggcaacacct tatccgggtg
    10561 ataaggtgat tattaccgaa ggcgcgttcg aaggctttca ggccattttc accgaacccg
    10621 atggtgaggc tcgttccatg ctattgctta atcttattaa taaagagatt aagcacagtg
    10681 tgaagaacac cgagttccgc aaactctaaa acgcaatccc aaacagtgtt ttgacattgg
    10741 catccgtggt ggcagccagc catgcggcat cttctccacg ccagtgcgca atacgttgca
    10801 aaatatggtg cagatgggct ggctcgttgc gccgggatga tggctttggc gtgaaatcgc
    10861 gcgggagcag atacggcgca tcagtttcga tcagcaattt ctccgccgga atcaacggta
    10921 acaattcccg cagctccagc ccgcgtcgtt catcgcaaac ccaaccggta atgccgatat
    10981 aaattccacg cgccacgcac gcctgcatct cttcgcgtgt gccggtaaag caatgaagaa
    11041 ccgcaccagg cagtttatcc agccacggct ccagcaatgt cataaaccgc tcgtgggcat
    11101 cgcgacagtg cataaatacc ggcatgttta attctgcggc aatgcgtagc tgagcaacaa
    11161 aagcgcgttc ctgctcttcc ggcgtcgaaa agttgcggtt aaagtcgaga ccacattcac
    11221 caatcgccac cacttctggc tgcacggcca gctcaataat cgcttcttca gtcgcagctt
    11281 gccactggct gctgtcgtga ggatgtacgc ccgccgttga ccaacaggac gaatactgac
    11341 gcgccagctt ttgcgcctgc tggctttcac gcaggttagt gccggtgatg agtagcccat
    11401 taactcccgc gtcaaaagcg cgcgctacaa catcatcacg gtctttcgca aattgcgaac
    11461 tggtcaaatt aacgccgata tcaaacatcc tgtactccat atgacaaccg ccctgacggg
    11521 cggttgaatt tattcttcag ttttttcgct ttctgcttca gcgtcgtttt cctcttcccg
    11581 atttcgccct ttaccaacgt aaaagcgtga gaagaagaca ccgatttcaa acagacagta
    11641 catcgggatc gccaatagcg tttgcgagaa gacatccggc ggcgtcagca acatcccgac
    11701 aacgaatgca ccaaccagca catacgggcg ttttttgcgt aagtcttctg gcgaggtaat
    11761 ccccatccag cacagcagca caattgccac cggcacttca aaggagacac caaacgccat
    11821 aaacagcgcc ataacgaagc ttaaatagct ggcgatgtcg gtggatacct gcaccccttc
    11881 cggcgcggta ttggcaagga agccaaatgc cagcggaaag accacaaagt aggcgaatgc
    11941 catgccgata taaaacagca gagagctgga aaccagcagc ggcaccacca ggcgacgttc
    12001 atgcttatac agcgctgggg cgataaatgc ccacacctga tagagaatca ccggcgctga
    12061 cagaatcagc gacaccataa aggtcagctt gatcggcgta aagaacggcg aggccacgtc
    12121 ggtggcgatc atcgttgaac cttgcggcaa ctgcttgatc aatggcgcgg ataccaggtg
    12181 atagatgtca ttggcgaaat agaccagaca caggaatatc acgatcaccg cgataatgca
    12241 gttcagcaga cgcttacgca gctcaattag atgcgtgata agcggttgag tatcttctac
    12301 agacatgttt acggtttatc actcgacgaa ggggaaggtg cagcggtttt cggttcagcg
    12361 tccgcagcag gttttaccac cggctctggc gtggtttctg gcttctgttc cggcgaactg
    12421 gcctgcgttt gtgcagcggc aggcgttacg ccctcatgcg cagcttcatt atctttcacc
    12481 accgggttat ggatggtgtg cgcttcatcg ctcgcctttt ctggatcgtt tgcaacgtag
    12541 gaacgcttca tcgactccgc ggcctggcgt agttcatcca tcgacgcttt cagttcgggc
    12601 gtcaggttag tgaggctcgc cttttcaacc tttttcagac tgtcctgaaa ctcctggagt
    12661 tttaactcct ggatcagttc gttctgcacc gttgtcgcca gtgaacgcaa cgcgcgaatc
    12721 cagcccgcta ccgtttttac cgccacaggc agtcgttgcg gccccagaac gacgaggccg
    12781 atgatgaaca ccaatagcag ttcgctaaaa ccgatatcaa acacggatta cacctgctct
    12841 ttatcgtggc gcttcgcgtc ttctgtttta gcctgttcct gattcgtatc cgcctgctta
    12901 tcggcgatag ttttcgcagt aaaatcagca tcctgactgg ttttatcctg ctttggttca
    12961 tcatcgctca ttgctttttt aaagcctttg atcgacgcac caagatcgga accgatggag
    13021 ccgagctttt tggtgccaaa aagcagtaca acgatgacgg caataatcaa taactgccaa
    13081 atactgatac cacccataca tgttcctctg tggtagatga tgattaaaca aagccgcatt
    13141 atacgttaca cggcccgcct tgagcgatga aaaaatcagc gtgttttgcg ccaaccgaca
    13201 aaccaggcga tcagaccacc tgccattaac cagccgggca tcagccccca ctcaggtcgg
    13261 ctgaccaaca agaatgtgcc acttaatact aacgtagcgc caattccgag aaaataacgc
    13321 gattgtccct gacgtacatg atttgactga agctcgcggg caatcttatc aacactgtgc
    13381 tgtaaatact tgccctggcg caaactgtcg taaaccaatt caggcagttc tggcattttt
    13441 tcgacccaga acggcgcttt ttctttaaat gctctcacca gcgcaggaat accgacctga
    13501 tctttaatcc acgactccag gaaaggcttc gctgttttcc ataaatcgag ctgcggataa
    13561 agctggcgtc ctaccccttc gacatagagc agggttttct ggagtaacac cagttgcggc
    13621 tgcacttcca tattgaagcg acgcgccgta ttaaacagat ttaacagtac atgtccaaac
    13681 gaaatttcgg ccagcggttt ctcaaagata ggttcacaga ccgtacgaat ggcaaattcg
    13741 aactcttcaa cgttggtatc tggtggcacc cagcctgaat cgacgtgtag ctctgccact
    13801 ttgcgatagt cgcgattaaa gaaggcgata aagttttctg ccagatagcg tttatcttct
    13861 ttgtttagcg agccaacaat cccgcaatca atgccgatat atttcgggtt ttccgggtgt
    13921 tcatagctta cgaagatgtt gccagggtgc atatcggcat ggaaaaagct gtcgcgaaag
    13981 acctgagtga agaacacctg cacgccgcgt tccgccagca atttcatgtt agtgccgttt
    14041 ttctccagcg ccgcaacatc agacaccgga atgccgtaaa tgcgctccat caccatcatc
    14101 ccttcactac aatagtcagg gtaaacttcc gggatgtaga gcatcgggct gtcttcaaaa
    14161 ttgcgccgaa gctgaatggc gttggcagat tcccgcagca aattcagttc atcaatcaag
    14221 gtcttttcgt actcgcgcac cacttcggtt gggcgcagac ggcgaccatc cggcagcaaa
    14281 cgcggcaccc agcgagccag acggtagata agtttcagat ccgctttaat gaccggcaaa
    14341 atatccgggc ggatgacttt aatcaccacc tctttaccat tcgatttcaa tcgcgcggta
    14401 tgaacctggg cgatagaagc agaagccagc ggcttgattt caaaatcgtc aaaccacgct
    14461 tctaccggca agccgcccat tgcagcttca atctgctgct tcgccagctt gccatcaaac
    14521 ggagctactt tgtcctgcaa taacgccagc tgatcggcaa tatgcggtgg aaaaagatcg
    14581 cggcgggttg ataacatttg cccgaacttg atccaaaccg gccccagttc ttgcagggcc
    14641 agtcgtagtc gctcacctaa aagtttgtct ttatgccgat ttggcatcca gaataatgag
    14701 tatcgccata gccgtagcgg cagggtgata cgcattttgg ggatcagttc atcaagtccg
    14761 tagcttaaaa aagtgcgaat gatgaaatat aggcgccgta cttcacctgg cgtcatttag
    14821 cctccagttt ttccagccgt ttggtcaggg catcaacagc acgctcgacg gcagccgtct
    14881 cttccgcaaa ccaggccact tcaagcggac cgggtgccat acgccactct tcagtaatgg
    14941 cttccgccac ataacgttgc tggcgcttaa tgccgtgatg caggaacttt gcgcctccgc
    15001 gcatggcttt gctgattcct tcagcggcga tatcaccggt ataaggggcc agcagttccg
    15061 cagggtcgaa ctctgccaga tctgccagcg caacgaagtt ttgcaccacc tgaatatcgc
    15121 cctgcacttc cagctcacca ctgcgaatca gtgcggtaag ctgctggcga tcgcgaagtt
    15181 tcggcaacac actggcgtag gcgataacgg tgcagtcagc atcgcctgcc cattcgccca
    15241 gtacatcaac ctggcgttcg ctgaacacca gaatcaatga cgtcgaaaag ccttttacct
    15301 ccacgcgcaa tactttaccc agcagacgcg agcgggctgt tttcagcgcg ggtgagcgat
    15361 acaggaaggt gttgagcaga ctttcaattc ctgccgtcac taaaggttta aaaggcattt
    15421 ccggtctcct gtcagaactt ataaccacga tgcagcgcca caacccctgc cgtcagattg
    15481 tagtagtcga cactttcgaa tccggcatcc tgcatcatgg ctttcagggt atcctgatcg
    15541 ggatgcatac ggatggattc tgccagataa cggtagctgt cggcgtcgtt cgcgaccagt
    15601 gagccaatac gcggcagcac atggaaggag tatgcatcat aggctttgct cagcggctcg
    15661 ataattggct tcgagaactc aagcaccagc aggcggccgc cgggtttcag cacgcgatac
    15721 attgaacgca gtgctttatc tttgtcggtg acgttacgca gaccaaacga aatggtgatg
    15781 caatcaaagg tgttatccgg gaacggcagc gcctcagcgt tcgcctgaac atactcaacg
    15841 ttgccaatca caccgatatt acgcagcttc tcgcgtccca ttttgagcat ggattcattg
    15901 atatcagcaa ggaccacttt gccagtttct ccgaccaggc gggagaattt cgctgtcagg
    15961 tcgccggtgc caccagccag atccagcacg gtctgcccac ggcgtacgcc gctgcaatca
    16021 atcgtgaatc gcttccacaa acgatgaata ccaaatgaca tcaaatcatt catgacatcg
    16081 tattttgatg ccacggaatg gaaaacgtgg gcgaccatat ccgctttttg ttccttcgcg
    16141 acggtctgaa aaccaaagtg cgtcgtttct tgtgacttat ccaccatctc aatgcctgct
    16201 catcaaaaaa ttgttccaga agtgtaacag attgggcgtc gatgccctag atttctaccc
    16261 ggcttaacta cccctaatgg gctagcgcga ctgctgatta tattcatcat cgcgttgata
    16321 agcttcatca ttcggctgct ccggaaccga ccgaagtcga tactcttcat cctggctcac
    16381 cgcctgttca gccaaatccg gattaatctc gcgtttaatt tctactccta aaccgcgaaa
    16441 cgcttctgcc tgcgccagca catttccgcg acctgaagag agttttttca ttgcctggcg
    16501 gtagttatcc tgcgctttgt cgagactttg accaatcgcg gacatgtcat cgatgaacaa
    16561 acgcatcttg tcgtacagct tgctggcacg atcggcgatt tgctgggcgt tgcggctttg
    16621 atgctcataa cgccacaggt tggcaatagt acgcaacgcc accagcagcg tagtcgggct
    16681 aaccagcatg atattgtttt tcaacgcttc ggtgatcagc tccggctggc ggtcaagcgc
    16741 cagtaaaaaa gcgggttcaa cgggaataaa catcagcacg taatccagag ttcgcagccc
    16801 cggcagctgt tgataatctt tgcgtcccag caaacggata tggttacgca ccgacgcgat
    16861 atgttcctgt agcgcgcttt cgcgggtgta gtcgtcttcg gcgttaaaat agcgttcata
    16921 ggcgaccagc gtcattttgg cgtcgatcac cacatctttt ccctgcggca gccgcacgat
    16981 gacatccggc tgcatccgcg agcgggcgtc attttcgatg ctgacctggg tttcatattc
    17041 atacccttca cgcagaccgg aagcctccag cacccgcgtc aatactacct cgccccagtt
    17101 gccctgggtt ttattgtcgc ctttcagcgc gcgcgtcagg ttgatcgctt cctgggccat
    17161 ttgcgcgttg agttgctgga gattgcgaat ttcgtgggtc agggtatggc gttcttgtgc
    17221 ttctttaccg aagctgtcct gaacctgacg gcggaaaccg tccagttgtt cacgtagcgg
    17281 cgacaacagg ctgttcagac tctgacggtt ttgctcatca acccggcgat tgctgtgctc
    17341 aaaaatacgg ttggcgaggt tttcaaactg ctcactgagg cgctgctcgc tgttaatcat
    17401 ctggcgaatt ttatcgtcag catgttgctg tgcggcttcc atccgcgtgg ttacttcacg
    17461 cagatcggcc tccagagagg tgttaatact ttgcaggctg cgcacttcgt tattgagtaa
    17521 ctcgcactct gcacgccagt gctcgctttg ggtaatttgt tgttttgccg cgcttaactc
    17581 cgcgaccatc tcttcacgtt cagctaattg ctcggctttt tgctgcgcat gttgataact
    17641 ggcaaacagc cagccaattg ccacacccac caacgcaata actgcgtaaa ccatgattga
    17701 gaaatccaca acgcctcctg acgcaacaac ttacccgcac aaaatagaat tgtgctgtac
    17761 aaacgtccag ttggaaaggc atttcctgcg aggcgctacg caaaaataca aaaggccgaa
    17821 cgcgtcggcc ttcagacagg agaagagaat tacagcagac gacgcgccgc ttccaccacg
    17881 attttcaccg cttggctttc agtttgtttc atcgtctcag cattcgggat ctcttgctgg
    17941 gtgcggttaa cgataacacc cgctaccata ccggcacgca ggccctgact tgcacacatg
    18001 gtcagcaggg ttgcagattc catttcatag ttcattacgc ccatcgcctg ccactcttcc
    18061 atagaacctt taaagtgacg aactacgcga ccagagtaag tatcgtaacg ttcctggcct
    18121 gggtagaagg tatcagaaga agctgtcacg ccaacgtgag ttgtcgcgcc aatggatttc
    18181 gcagcttcaa ccagcgcagt cgtacattcg aaatcagcga cagccgggaa ttccagcggt
    18241 gcgaagtgca ggctcgcgcc atccagacgg acagacgccg tggtaaccag gacatcaccc
    18301 acattaatat gcggctgaat agcgcccgtt gtaccgatac gcaggaaggt gcgaatgccc
    18361 agctgtgcca gctcttcaac agcaatagag gtagacgggc cgccgatacc ggtagagcag
    18421 acgataacag gtttaccatc cagctctgca cgccaggtgg tgaattcgcg gtgagatgcc
    18481 agcttaaccg gcttatccat cagcgcggcg atcttttcca cacgatccgg gtcgccaggg
    18541 acgatggcaa gcgtagcccc ttgtaaatcg tttttagtga ggccgagatg aaaaacatca
    18601 gacttggaca tatacaactc ctctgtgaat cggtttagtc agaagaagca aaaagacact
    18661 ttaccgaagg gtttaacatt ttttcgtgat actcatcacc atgacgcaaa tgcgttgcat
    18721 aaatttaccc accaaaagtg atgcaaatca cataaatcac tcttcattct gcaggactgc
    18781 ataaaagatg agccgattca ggcaatgtga tttgttgcat gctgactata gttaacattg
    18841 cgcaacattt ttataagggt acggagaata ccatggcaac aacacaacaa tctggatttg
    18901 cacctgctgc atcgcctctc gcttcgacca tcgttcagac cccggacgac gcgattgtgg
    18961 cgggcttcac ctctatccct tcacaagggg ataacatgcc tgcttaccat gccagaccaa
    19021 agcaaagcga tggcccactg ccagtggtca ttgtagtgca ggaaattttt ggcatgcatg
    19081 aacatatccg cgacatttgt cgccgtctgg cgctggaggg gtatctggct atcgcacctg
    19141 aactttactt ccgcgaaggc gatccgaatg attttgccga tatccccacg ttgcttagcg
    19201 gtctggtagc aaaagtgcct gactcgcagg tgctggccga tctcgatcat gtcgccagtt
    19261 gggcttcccg caacggcggc gatgttcatc gtttaatgat caccggattc tgctggggtg
    19321 gacgtatcac ctggctgtat gccgcgcata atccacagct aaaagctgca gtggcgtggt
    19381 acggcaaact gacgggcgac aagtcgctaa attcaccgaa acaacctgtt gatatcgcaa
    19441 ccgatcttaa cgcgccggtt ctcggcttat atggtggtca ggataacagc attccgcagg
    19501 agagcgttga aaccatgcgc caggcgctgc gggctgctaa tgcgaaagca gagattatcg
    19561 tgtacccgga cgccgggcat gcattcaacg ctgattatcg cccgagctat catgccgaat
    19621 ctgcgaaaga tggctggcag cgtatgctgg aatggtttaa gcagtatggt gggaagcgga
    19681 gttagtttcc tcttagtcta tatttgagat ggtgagtgaa cgagtgcgtt ttgctgccat
    19741 ctctgtcccc caaaaaataa tcagtatgaa ttgattggag attacgactc cgctatttga
    19801 ccttaagaag gtcggcaagt catagtcata aatgagtctc ttcaatggaa gacaccttag
    19861 agatttacca tctactccca ttcacaaaca aacattaatt attatcaaca cattcacatt
    19921 aattataaat catctcagac cttcctgata aaaagatatt aaagtgaaac attttgtttt
    19981 tcacatttta gttcatcact tttaggcgta aataaaggta aaaaaaacaa caatactatt
    20041 tctctttcac aaaagaatta acaaaaggtt ttttaaggat gagtgatatt atttacttga
    20101 ccataaccgg agaacaacaa ggatgtattt cctctctttg tggaacgagc gcatcaatag
    20161 gtaatcgctg gcagattggc catgaagatg aaatcttcgc gttctcgctc tctaatagta
    20221 ttacgaatgc aggcaaaggg tcacaattgc atggccttag tttctgtaaa ttcttattaa
    20281 cgctattaac aataacgagc agttgttcat ggcgtttgac ttctatcgga tcaatagatt
    20341 tggtcgatgg gagaaatatt acaacattca actccgcggc gcgctcttat cagcaatcaa
    20401 tcacctgttt actgagaaca atctggatac agaagccatc actgtcagtt atgaatatat
    20461 actctccagg catctgattg ctaatactga atttagttat ttggcatttc ccgataacta
    20521 caaccgtctg tttataccac ggccaaaaac taaagctggt aatggttcaa aacacttaat
    20581 agtaaagcgg ttggcagact gcttgctgct ggcggcatat acaatgggaa tatcgaagga
    20641 ttcagggaaa cagcagacaa gttgggggga gatgcacctg ctggttacga tcaatttatg
    20701 gataacaaag ggttactcat cgctggtgct tcaatagttg ctggagcagt tctggggaga
    20761 atgagactcc ctgatttgga atcacttgag catttcggag ccagaggagc agtatcagga
    20821 a
//
LOCUS       DAJVUJ010000061        20742 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.061, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000061 DAJVUJ010000000
VERSION     DAJVUJ010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20742
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.061"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(<1..271)
                     /locus_tag="OQQ48_002070"
     CDS             complement(<1..271)
                     /locus_tag="OQQ48_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1952847.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAILRCTGNSGHYHLFFF"
     gene            complement(524..994)
                     /gene="bcp"
                     /locus_tag="OQQ48_002071"
     CDS             complement(524..994)
                     /gene="bcp"
                     /locus_tag="OQQ48_002071"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HCS1952848.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(994..1566)
                     /locus_tag="OQQ48_002072"
     CDS             complement(994..1566)
                     /locus_tag="OQQ48_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HCS1952849.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            1712..2590
                     /gene="dapA"
                     /locus_tag="OQQ48_002073"
     CDS             1712..2590
                     /gene="dapA"
                     /locus_tag="OQQ48_002073"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCS1952850.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            2607..3641
                     /gene="bamC"
                     /locus_tag="OQQ48_002074"
     CDS             2607..3641
                     /gene="bamC"
                     /locus_tag="OQQ48_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HCS1952851.1"
                     /translation="MAYSVQKSRLAKVAGVSLILLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGVRTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTIDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSASDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            3854..4567
                     /gene="purC"
                     /locus_tag="OQQ48_002075"
     CDS             3854..4567
                     /gene="purC"
                     /locus_tag="OQQ48_002075"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCS1952852.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKRFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            4735..5598
                     /locus_tag="OQQ48_002076"
     CDS             4735..5598
                     /locus_tag="OQQ48_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HCS1952853.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHNMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            5613..7628
                     /gene="tmcA"
                     /locus_tag="OQQ48_002077"
     CDS             5613..7628
                     /gene="tmcA"
                     /locus_tag="OQQ48_002077"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HCS1952854.1"
                     /translation="MAELTALYTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALL
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFDDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQYFLQAAGENEIAGALWLVDEGGLSQQLSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGVGQQLIASALQYTQDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYCLRRDAQALAQWNGETLPVD
                     PLNDIVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLAGRKMLLVRQREEAAHALFALNDVRTERLRDRITQWQFFH"
     gene            7702..8400
                     /gene="ypfH"
                     /locus_tag="OQQ48_002078"
     CDS             7702..8400
                     /gene="ypfH"
                     /locus_tag="OQQ48_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCS1952855.1"
                     /translation="MKHDHFVVQSPDKPVQQLLLLFLGVGDNSVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(8510..8710)
                     /locus_tag="OQQ48_002079"
     CDS             complement(8510..8710)
                     /locus_tag="OQQ48_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HCS1952856.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(8738..9865)
                     /gene="dapE"
                     /locus_tag="OQQ48_002080"
     CDS             complement(8738..9865)
                     /gene="dapE"
                     /locus_tag="OQQ48_002080"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HCS1952857.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(9869..10225)
                     /locus_tag="OQQ48_002081"
     CDS             complement(9869..10225)
                     /locus_tag="OQQ48_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HCS1952858.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPSIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            10606..10665
                     /gene="ypfM"
                     /locus_tag="OQQ48_002082"
     CDS             10606..10665
                     /gene="ypfM"
                     /locus_tag="OQQ48_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HCS1952859.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            complement(10764..13872)
                     /gene="acrD"
                     /locus_tag="OQQ48_002083"
                     /pseudo
     CDS             complement(10764..13872)
                     /gene="acrD"
                     /locus_tag="OQQ48_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
     gene            complement(14035..15735)
                     /gene="narQ"
                     /locus_tag="OQQ48_002084"
     CDS             complement(14035..15735)
                     /gene="narQ"
                     /locus_tag="OQQ48_002084"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HCS1952860.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQHLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            15941..17920
                     /gene="aegA"
                     /locus_tag="OQQ48_002085"
     CDS             15941..17920
                     /gene="aegA"
                     /locus_tag="OQQ48_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HCS1952861.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPVEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            17988..18563
                     /gene="nudK"
                     /locus_tag="OQQ48_002086"
     CDS             17988..18563
                     /gene="nudK"
                     /locus_tag="OQQ48_002086"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HCS1952862.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQTSHLMD"
     gene            18689..19732
                     /locus_tag="OQQ48_002087"
     CDS             18689..19732
                     /locus_tag="OQQ48_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HCS1952863.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPEASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKNPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(19842..20742)
                     /locus_tag="OQQ48_002088"
                     /pseudo
     CDS             complement(19842..20742)
                     /locus_tag="OQQ48_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="catalyzes the formation of ribose 5-phosphate and
                     xylulose 5-phosphate from sedoheptulose 7-phosphate and
                     glyceraldehyde 3-phosphate; can transfer ketol groups
                     between several groups; in Escherichia coli there are two
                     tkt genes, tktA expressed during exponential growth and
                     the tktB during stationary phase; frameshifted;
                     incomplete; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase"
BASE COUNT         5018 a         5300 c         5550 g         4874 t
ORIGIN      
        1 aaaaaaagaa taaatggtag tgtccgctat tgccagtaca cctcaggatc gcatcatccc
       61 tctgggagat ggcattgacc gggcagacct gcaggcaagg ggcttcctca cagtgatgac
      121 acacgacagg ggcagtgatt gttgacgtct tcgccagggc caggcgcggg tgttgctgta
      181 aaccttgcgt tttatgcacg tccgaacagg cagcgagaca ggtattacat cctgtacacc
      241 acagtggttc ggccaccaca aagcggttca tgtcggctcc ttgaaaatga tatgaaacat
      301 tgcatttcat attgcataaa ggatgccagt ctgattaatt taatatattc aataaattat
      361 gtattaacag ggttctgaac ggtacaaaaa tgatggtcat taacgttcag caaaaaatgg
      421 attcgacacc cgtgtcgcag gggaaagcga gaatgtgatc ttgatcacga gaagtgaggt
      481 gtgctgaccg caagcgcagc cagcacggaa tggagcaaag taatcaggcg tgttctttca
      541 gccagttcag cacaacgtcg tggtgattgc tggttttaaa atcgtcaaag acatgttcga
      601 ttttgccatc agcgtcaatc aggaagctga tgcgatgaat accatcgtag gttttgccca
      661 tgaaggactt ttcaccccag acgccgaatt gttcgcacac ctggtggtcc tcatcagaca
      721 ggagcgtaaa gttaagcagc tctttttccg caaaacggga gagtttttcg ggtttatcgg
      781 tgctgatacc cagcacatca acgcccgctt ttttcaactc atccatgtta tcgcgtaagc
      841 cgcaggcctg tacggtacag ccgggggtca tggctttcgg gtagaaataa accagaacac
      901 gctgtccctg gaagtcggtc aaattaactt gttctccgtc ttgatccggc aagctaaatt
      961 tcggtgcgat atcaccggct ttcagtggat tcattactta actccatcct gttcatcatg
     1021 ttgggaataa ttgacgacgt taatactgcc ttgtgcattg agttctgtac atagggcttt
     1081 gaacgcttgc tcaatatttg ctgcgtccgc agatgcgggg ctgtgggcgg tgatctgaat
     1141 atgcaactgc gcagcccgtt cattttcagc aggttgcgtg cgcgacacca gctccgcaat
     1201 gttcatatga tgcgcgtcga aaagtgctgt gaagcgttca attaaatgcg gggagtctgc
     1261 aacatcgacc tgaacccaga cagatgctgg cattggcgga cgcggacgcg ccgtcgtgcg
     1321 cttcatcacg attaaaagat ccagttcggc acctttcaac ggtaacgttg attcaatcag
     1381 agtaatggca ttccatgaac cggaaagcag catgataaac gtgaactctt ctcccagcat
     1441 cgccaggcga ctgtcttcaa tattacagcc gcaactactg acatgacggg tgatggtgtt
     1501 cacaattcca gggcgatcgg cacccaacgc agtgatcacc agataatgtt gcgatgacag
     1561 tgtcaaactg gttattcctt taaggggtga gttgttctta aggaaagcat aaaaaaaaca
     1621 tgcatacaac aatcagaacg gttctgtctg cttgctttta atgccatacc aaacgtacca
     1681 ttgagacact tgtttgcaca gaggatggcc catgttcacg ggaagtattg tcgcgattgt
     1741 tactccgatg gatgaaaaag gtaatgtctg tcgggctagc ttgaaaaaac tgattgatta
     1801 tcatgtcgcc agcggtactt cggcgatcgt ttctgttggc accactggcg agtccgctac
     1861 cttaaatcat gacgaacatg ctgatgtggt gatgatgacg ctggagctgg ctgacgggcg
     1921 cattccggtg attgccggga ccggtgctaa cgctactgcg gaagccatta gcctgacgca
     1981 gcgcttcaat gacagcggta tcgtcggctg cctgacggta accccttact acaatcgtcc
     2041 gtcgcaagaa ggtttgtatc agcatttcaa agccatcgct gaacatactg acctgccgca
     2101 aattctgtat aatgtgccgt cccgtactgg ctgcgatctg ctcccggaaa cggtgggccg
     2161 tctggcgaaa gtaaaaaata ttatcggaat caaagaggca acagggaact taacgcgtgt
     2221 aaaccagatc aaagagctgg tttcagatga ttttgttctg ctgagcggcg atgatgcgag
     2281 cgcgctggac ttcatgcaat taggcggtca tggggttatt tccgttacgg ctaacgtcgc
     2341 agcgcgtgat atggcccaga tgtgcaaact ggcagcagaa gggcattttg ccgaggcacg
     2401 cgttattaat cagcgtctga tgccattaca caacaaacta tttgtcgaac ccaatccaat
     2461 cccggtgaaa tgggcatgta aggaactggg tcttgtggcg accgatacgc tgcgcctgcc
     2521 aatgacacca atcaccgaca gtggtcgtga gacggtcaga gcggcgctta agcatgccgg
     2581 tttgctgtaa agtttaggga gatttgatgg cttactctgt tcaaaagtcg cgcctggcaa
     2641 aggttgcggg tgtttcgctt attttattac tcgctgcctg tagttctgac tcacgctata
     2701 agcgtcaggt cagtggtgat gaagcctacc tggaagcggc accgcttgcg gagcttcatg
     2761 ccccggctgg aatgattttg ccggtgacct ccggtgatta tgcaatcccg gtgaccaacg
     2821 gtagtggtgc tgtcggtaag gcgctggaca ttcgtccacc agcccagccg ctggcactgg
     2881 tttctggcgt gcgtacccag ttcacgggcg ataccgcttc attgctggtg gaaaatggtc
     2941 gtggcaatac tctgtggccg caggtggtta gcgtgctgca ggcgaaaaac tacaccatca
     3001 cccaacgtga tgatgctggt cagacactga ccatcgattg ggtacaatgg aaccgtctgg
     3061 acgaagacga gcagtatcgt ggtcgttatc aaatctctgt taagccgcag ggttatcagc
     3121 aggcggttac ggttaaactg ctgaacctgg aacaggcggg caaaccggtt gcagacgcgg
     3181 cttccatgca gcgttacagc acggagatga tgaacgttat ttccgccggt ctggataaat
     3241 ctgcctctga cgccgcgaac gctgcgcaaa atcgtgcctc caccactatg gacgtacaaa
     3301 gtgcagctga tgacaccggt ttaccgatgc tggtcgtacg cgggccgttc aatgtggttt
     3361 ggcaacgtct gccagcggcg ctggaaaaag tgggcatgaa agtgaccgac agcacccgtt
     3421 cgcagggtaa catggccgta acttataagc cgctgtctga cagcgactgg caggaactgg
     3481 gcgcgagcga tccaggcctg gcatccggtg actataaatt gcaggttggc gatttagata
     3541 accgcagcag cctacagttc atcgatccga aaggtcatac tctgactcag agtcagaacg
     3601 acgcgctggt agctgtcttc caggctgcgt ttagcaagta aaaatatagg gctggagtca
     3661 tccggccctt ttttctgata tgatacgcaa acgtgtgcgt cggcagaaaa atgcaatttt
     3721 agcggtaatt cgcacgaaat ttgtttgtcg gacgtaggtc ggataaggcg ttcttgccgc
     3781 atccgacaaa acatccggca caccagacag caaaagattt taaaacgtta attcacaccc
     3841 aggagtgata aagatgcaaa agcaagctga gttgtatcgt ggtaaagcga aaaccgtata
     3901 cagcacggaa aacccggacc tgttggtgct cgaattccgc aatgatacgt cagcagggga
     3961 tggcgcgcgc attgagcagt ttgatcgcaa aggtatggtg aacaacaagt tcaactactt
     4021 cattatgagc aaactggctg aagcgggtat cccgactcaa atggagcgtc tgctctccga
     4081 taccgaatgt ctggtgaaaa agctggatat ggtgccggtt gagtgtgtcg tgcgtaaccg
     4141 tgctgctggc tctctggtga aacgtcttgg aatcgaagaa ggtattgagc tgaacccgcc
     4201 gctgttcgat ctgttcctga aaaacgatgc catgcacgat ccgatggtca acgaatctta
     4261 ctgcgaaacc tttggctggg tgagcaaaga gaacctggcg cgtatgaaag agctgaccta
     4321 caaagcgaac gacgtgctga aaaaacggtt cgatgatgct ggtctgattc tggtcgactt
     4381 caagctggag tttggtctgt acaaaggtga agtggtactg ggcgatgagt tctccccgga
     4441 cggcagccgc ctgtgggaca aagaaacact ggagaaaatg gacaaagacc gtttccgcca
     4501 gagcctcggt ggcctgatcg aagcctatga agccgtcgcc cgccgcctgg gtgtacagct
     4561 ggactgattt ttctgttcat catcttgccg tgcagctggc acggcaagac aaccgctctc
     4621 ggaatatatg cattgaacgc tcattcctcc gccatttcat cccgctttcc tgtggtaatc
     4681 ttcctctgaa ccacctacga tcatttctat aatgaatata ttgttgaggt atctatgcgt
     4741 tggcaagggc gacgtgaaag tgacaatgtt gaagacaggc gcaacagctc cggtggtccg
     4801 tctatgggcg gtcccggttt tcgcctgcca agcggtaaag gcgggctgat tttactgata
     4861 gtcgtgctgg ttgcaggcta ctatggtgtt gatttaaccg ggttaatgac tggacagccg
     4921 gtttcccaac aacaatcaac gcggtccatt agccctaatg aagacgaagc tgcaaaattc
     4981 acctcggtga ttctggcaac cacggaagac acctggggac aacagttcga gaagatgggt
     5041 aagacctatc agcaaccgaa gctggtcatg taccgtggaa tgacgcgcac cggctgcggg
     5101 gcgggccagt ccattatggg gccgttctat tgcccggcgg atggcacagt ttatatcgat
     5161 ctctccttct atgatgacat gaaagacaaa cttggcgcgg atggcgattt tgcccagggg
     5221 tacgttatcg cccatgaagt cggtcatcat gtgcagaaac tgttaggcat cgagccgaaa
     5281 gttcgtcaac tgcaacaaaa cgcgacgcag gcggaagtga accgcttatc tgtgcgtatg
     5341 gaactccagg ccgactgttt tgccggtgtc tgggggcata acatgcagca gcaaggcgtt
     5401 ctggaaaccg gcgatctgga agaggcgctg aatgcggcgc aggccatcgg cgatgaccgt
     5461 ttacaacagc aaagtcaggg gcgagtagta ccagacagtt tcacacatgg cacttctcag
     5521 caacgctaca gctggtttaa acgtggtttc gacagcggcg atccggcaca atgcaatact
     5581 tttggtaaaa gcatttaatt tccggggcag ggatggctga actgactgcg ctttacacat
     5641 taacagcgca aatgaaacgt gaagggatcc gccgcttgct ggtgttgagc ggggaagagg
     5701 gttggtgttt tgatcatgcg cttaagttgc gtgatgcctt acttggcgac tggctgtgga
     5761 tttcgccgca gccagatgct gaaaaccact gttctccctc ggcgctacaa actttacttg
     5821 ggcgcgagtt ccggcatgcg gtattcgacg cccgccacgg ctttgatgcc gctgcctttg
     5881 cggcacttag cggaacgttg aaagcgggaa gctggctggt tttgttactc cctgtatggg
     5941 aagagtggga aaaccaacct gatgccgact cgctgcgctg gagtgattgc cctgacccta
     6001 ttgcgacgcc gcattttgtc cagcatctca aacgcgtact tacggcggat aacgacgcta
     6061 ttctctggcg gcaaaaccag ccgttctcgt tggcgcattt tactccccgt actgactggc
     6121 accccgcgac cggcgcacca caaccagaac aacagcaact cttacagcag ctactgacca
     6181 tgccgccggg cgtggcagcg gtaacggctg cgcgtgggcg cggtaagtcg gcgctggcag
     6241 ggcaactcat ttctcgtatt gcgggcagtg cgattgtcac cgcgcccgca aaagcggcaa
     6301 cggatgtact ggcacaattt gcgggcgaga agtttcgctt tattgcgccg gatgccttgt
     6361 tagccagcga tgagcaagcc gactggctgg tggtcgatga agccgcagcc atacctgcgc
     6421 cgttgttgca tcaactggta tcgcgttttc ctcgaacgtt gttaaccact acggtgcagg
     6481 gctacgaagg caccggacgt ggttttttgc tgaaattttg cgctcgcttt ccgcatttac
     6541 accgttttga actgcaacag ccgatccgct gggcacaggg atgcccgctg gaaaaaatgg
     6601 ttagtgaggc actggttttt gacgatgaaa acttcaccca tacaccacaa ggcaatatcg
     6661 tcatttccgc atttgaacaa acgttatggc gaagcgagcc agaaacgccg ttaaaggttt
     6721 atcagttatt gtctggtgcg cactaccgga cttcgccgct ggatttacgc cggatgatgg
     6781 atgcaccagg gcaatatttt ttacaggcgg ctggcgaaaa cgagattgcc ggagcgctgt
     6841 ggctggtgga tgagggggga ttatctcaac aactcagtca ggcggtatgg gcaggttttc
     6901 gtcgcccgcg gggtaatctg gtggcccagt cgctggcggc gcacggtagc aatccactgg
     6961 cagcgacatt gcgtggacgg cgggtcagcc ggatagcagt tcatccggct cgtcagcgcg
     7021 aaggcgttgg gcaacagctc attgccagcg ctttgcaata tacgcaagac ctcgactatc
     7081 tttcggtgag ttttggttac accggggagt tatggcgttt ctggcaacgc tgcggttttg
     7141 tgctggtacg aatgggtaat catcgtgaag ccagcagtgg ttgctatacg gcgatggcgc
     7201 tgttaccgat gagtgatgcg ggtaaacagc tggctgaacg tgagcattac tgtttacgtc
     7261 gcgatgcgca agctctcgcg cagtggaatg gcgaaacact tcctgttgat ccactaaacg
     7321 atatcgtgct ttctgacgac gactggcttg aactggccgg ttttgctttt gctcatcgtc
     7381 cgctattaac gtcgttaggt tgcttattgc gtctgctaca aaccagtgaa ctggcattac
     7441 cggcgctgcg tgggcgttta cagaaaaatg ccagtgatgc gcagttatgt accacactta
     7501 aacttgcagg ccgcaagatg ttactggtcc gtcagcggga agaggccgcg catgcgctgt
     7561 tcgcacttaa tgatgttcgc actgagcgtc tgcgcgatcg cataacgcaa tggcaatttt
     7621 ttcactgact ccttcaattt tctcccatgg tcattgtgag ccagcggcgt aaaattaatg
     7681 acatcagtta aggagttcgc catgaaacat gaccattttg ttgttcaaag cccggataaa
     7741 cctgtgcaac agttgttact gcttttcctc ggtgtcgggg ataactcggt ggcaatgggg
     7801 gaaataggca gttggtttgc accgctgttt cctgatgcgc tggtggtgag tgtcggtggc
     7861 gcggagccga gcggtaatcc ggcggggcgt cagtggtttt cggtgcaggg tattacggaa
     7921 gataatcgcc aggcgcgtgt ggatgccatc atgccgacgt ttattgaaac ggtacgctac
     7981 tggcagaaac agagtggagt aggggctaac gccacggcgt taatcggttt ctctcaaggc
     8041 gcgattatgg cgctggagag tattaaagcc gaaccgggcc ttgcttcacg cgtcattgct
     8101 tttaacggac gctatgcgag cctgccggaa accgcgtcta cagcaacgac aattcacctg
     8161 attcatggcg gtgaagatcc ggttatcgat ctggcgcatg cggttgcggc gcaggaagcg
     8221 ttaatcagtg ccggtggtga cgtgacgctg gatatcgtgg aggatttagg ccatgcgatc
     8281 gacaatcgca gtatgcaatt tgccctggat cacttgcgtt ataccattcc gaaacattat
     8341 ttcgacgaag cgctaagtgg cggtaagcct ggcgatgatg atgtgattga aatgatgtaa
     8401 aagacagatt acctggcctg ataagacgcg tgagcgtcgc atcaggcatc gttcaccatt
     8461 gccggatgcg gcgttaacgc cttatccggc ctacggttga gcactcagct tacttcttcg
     8521 gttgatcctt tttcggccag tcgtcgtcat cgtcccactt atcgttgaaa tcacgatgcg
     8581 ggggaagctc cggcttgttg gcgagaaatt tcttgtggtc gacgcgcttg agatctttaa
     8641 tcacgttcag caggacgcct accaaaaaga caatcaccag aatccaccaa tatttagcca
     8701 gccagtccat gcttatttcc tcttgcagaa ccactcatca ggcgacgagc tgttccatga
     8761 tacgttgata catacgggca agtagctgca ggtcggcagc gttcacacat tcattaattt
     8821 tatgaatagt ggcattgacc ggcccgagtt ccaccacctg cgcccccatg cgagcaataa
     8881 agcgcccgtc ggacgttccg cctgtggtca gtagctgcgg tttaatttca ttatagtgct
     8941 caaccgcgtt aacgaccgca tccaccagtt taccgcgcgc ggtcaaaaat ggctgcccgg
     9001 aaagccacca atccaccgta tagcgcagtt gatgtttttc aagcagggca agcacctgcg
     9061 ctttgatcat ctcatcagtc agttcggtgc tgaagcggaa gttaaactgc acaaacagtt
     9121 cacccggaat aacattgtta ctgcccgttc ccgcctgaat attagcaatc tgcatactgg
     9181 tcgccgggaa aaattcattg ccctgatccc actcaatagc cactaattca ttaaggaaag
     9241 gtgctgcgcg atgtactgga ttgtcagcca gatgcgggta ggccacatgc ccctgaacgc
     9301 catgaatggt aaggttgcag gttaacgatc cgcgacgacc atttttcacc acatcaccaa
     9361 ccacttcgat actcgacggt tcgccgacca ggcagtaatc gagacgctca ttacgtgcca
     9421 tcaacgcttc gacgactttt accgtaccgt tgtgggcact ggcttcttca tcagaggtga
     9481 tcagaaatgc cagtcgcccc gtatggttgg gatgttgtgc gacaaagcgt tctgccgcta
     9541 ccaccatcgc cgccagcgag cctttcatat ctgccgcacc gcgcccgaat aacatgccat
     9601 cacgaatggt gggttcaaac ggcggattga tccaacgatc ggcgtcgcca ggcggcacca
     9661 cgtcggtatg cccggcaaag gctaacgttt caccttgccc acgccatgcc caaaaattct
     9721 gcgtatcggc aaagtccatg cgttcaacgg taaaaccgat cgcctgcaaa cgttcaatca
     9781 acaaagcctg gcatcctgcg tcatcaggac tcagggaagg gcggcgaata agctgttgtg
     9841 tcagctcaat aaccgggcac gacatagact acacctcatg gaaaaattgc tgataactgg
     9901 aatcactgaa acccagcagc ataggcttac caggcgcgca gagcaatgga cgtttgataa
     9961 ttgaaggcat ttcagtcatt aatgccgccg cagaggccgc atcggtgatt ttattgcggg
    10021 tggtttcgtc cagtttacgc caggttgtac cacgggtgtt cagtaacgct tcccagccta
    10081 attcgttgat aaaaccgttc aataattcgc tgtccagccc atcgacgcgg taatcatgaa
    10141 aacgatagtc gatgttattg gcttccagcc aacggcgagc ctttttaatg gtgtcacaat
    10201 ttttgatgcc gtaaagtgta accataatga atcctttttc gatctatgag aataaatact
    10261 catttaaggg tgaaaacatt ttgcctgcaa atgattatca ggaaatataa cattatcgaa
    10321 gtattcaaat taacaactat taaataatat taaacatttt taacgtaaac attaagaata
    10381 ttcatcttgt tatctgtctg tattttaacc agaattaaat cagtattaga tgccgtatga
    10441 atatctcgaa atgttgctgt gtatcacata caaattatca ccgcaggacc atagtattta
    10501 cataaggttt gtcccccgga ggtagtcatc gcagcaatcg agtaaattag cttcaccacg
    10561 gtatattgtt tccgtagaat acccataatt ataagagagg ttgttatgat tgaacgtgaa
    10621 ctggggaact ggaaagactt tatcgaagtt atgcttcgta agtaattcct gagacaaagt
    10681 acaaacagca agaacccgct gagcaggttc ttaatcggaa agttgaaaaa ggcgacacat
    10741 tagcatgtcg ccttttttat tgcttattcc gggcgcggct tcagcgggaa gcggcggcgc
    10801 accagtacaa agaacagcgg cacgaagtaa atagccagaa tagtggccga aatcatcccg
    10861 cccattacgc cagtacccac cgcatgctga ccaccggaac cggcacccgt gctggttgcc
    10921 attggcaata cgccgaagat aaatgccagc gaggtcatca gaatcgggcg taaacgctga
    10981 cggcaggcgt ggagcgtcgc ttcaaacagg tcgtggcctt tttggttcat ctcgttagca
    11041 aactcgacga tcaggatggc gtttttcgcc gataaaccaa tgaccgttaa caggcccacc
    11101 tggaagtaaa cgtcgttttc cagcccgcgc atccaggttg ccagcagcgc gccgataacc
    11161 cccagcggca cgaccagcat taccgagaac ggcaccgacc agctttcaac gccgccagac
    11221 acaggaatac caccagcaag gaaatggcgt acagcgccgg agcctgcgcg ccggaaagcc
    11281 gctcctgata cgacatcgcc gtccactcca gaccaaagcc gtttggcaac tgcttcacta
    11341 acgattccat tatatccatc gccgtaccgg tactgacccc cggtgcggct tccccaacaa
    11401 tctcaaccgc agaataaccg ttatagcgtt ccagacgcgg cgagccagtc tcccagcgtg
    11461 aggtcgcgaa agcagagaag ggcaccatgc cgccatcttt atttcggaca taccagagat
    11521 taatgtcatc tggcagcatg cgatacggcg cagctgcctg cacatagact ttcttcacac
    11581 gaccgcgatc cataaagtca ttcacatagc tcgaacccca ggcggtttgc agtgtgtcgt
    11641 taatatcgtc gatagcaacg cccagcgcct gagctttacg ctggtcgata tcaatctgca
    11701 actgcggact gtcatcgagg ccgttatggc gcacacgggt tagctccggg ttttccgccg
    11761 ccagcgccag caactggtta cgtgctgcca tcagcgcatc gtgacctgct ccagcgtggt
    11821 cctgcaactc catatcaaaa cccgcagaac taccaagacc gctaattgct ggcgggctgc
    11881 tggcgataac gcgagcttct ttaatcttgt taaacgcctt tgttgcacgc tcgataatgg
    11941 caaacgaggt gccggtctta ctgtcgcgtt cgctccagtc tttcaggcgg ataaacattc
    12001 gcgccacgtt ttgcccgtta cccccagggc cagaaccaac ggtggcaaac accgacatga
    12061 tgttgtcttt ttcatgggtg aagtaatatt tctcgatttg ctcaacgact ttcagggtct
    12121 gttgttgggt tgaaccgctg ggcaattgta ccgaggtagt aaacatgcca cggtcttcca
    12181 gcggtaagaa cgacgtcggc aaacgcagga acaggaacac catgccgcca agcagcagga
    12241 cataaatcac aatccagcgc aggctgcggt ggagaatttt cgccacccct ttttcgtagc
    12301 gttcggcgtt gcggttaaac atctggttaa accaggcaaa aaagcctttt tgcccgtgat
    12361 gctcaccttt tttcagtggc ttaagtagtg tggcacacag agccggagtg aggatcatcg
    12421 ctaccagtac tgacagcacc atcgccgcaa caatggtaat agagaactgg cgatagatgg
    12481 caccggtggt gccgccgaag aaggccattg gtacaaatac cgccgaaagc accatcgcaa
    12541 tcccgaccag tgccccctgg atctgcccca tcgatttacg tgtggcttcg cgaggagtga
    12601 gtccttcctc actcataata cgttcgacgt tttccaccac cacgattgcg tcatccacca
    12661 gcagaccgat cgccagtacc atcgcgaaca tggttaaggt gttgacgctg taaccgaagg
    12721 cgtaaagtac ggagaaggtt cccatcaaca ccaccggcac ggcgatagtg ggtatcagcg
    12781 tggcgcggaa gttttgcagg aacagataca taacgaggaa aaccagggcg atagcttcca
    12841 gcagcgtttt caccacgtct tcaatggagg ctttaacaaa cgaggttgtt tcatacgcca
    12901 ccttgtattc cagtccgtgc gggaagtact gcgccagctc gtcgagacga ttgagcacca
    12961 gctccctgtc gccatttcgt ttgcgccgga ggccagtttt acccccagcc cggaggctgg
    13021 cttaccattg aagcggctaa gataatcgta tttctccgcc cccatttcga cggtggcgac
    13081 atcgcccagc cttacctctg agccgtcttg attcacccgc aaggtgatat cgcggaactg
    13141 ttctggtgtt tgcagcagtg actgggcgtt aatggtggcg ttgagcgcct gcttatcgac
    13201 ggaaggtgta ccaccaagtt gcccaaccgc aatctgcgcg ttctgtgact caatggcatc
    13261 agtgacatct ttagccgtca tctggaaact gttgagtttc gccggggcca gccagatacg
    13321 catggaatat tgcgaaccat aggcatcgat atccccgacg ccattcacgc ggcttaacgg
    13381 gtcctgaata ttactggcaa cataatcggc aatatcctgt ttatccatcg aaccatcggt
    13441 agagacgaaa gcaatggtca gaatattggt atcgccggtt ttacgcaccg tcacgccctg
    13501 attttgcacc gcctgcggta actttcgcat ggctgattgc agctggtttt gtacttgctg
    13561 cacggcttcg tccggatcgg tgcctgcttt aaaacttaaa gtgacagatg cctgaccggt
    13621 gccactgctc tgagatgaca tatacatgag attatcgagg ccggtcatat tttgctcgat
    13681 aacctggggc acggtgtttt ccagcgtctg ggccgatgcg ccgggatagt tagcggtcac
    13741 tcgcacattc ggtggcgcga gatcggggta ttgttcaacg ggcaatgaaa aaatcgctag
    13801 ggtccctgtc agacacaaca ggattgccag cacccaggca aaaatggggc gatcaataaa
    13861 gaaattcgcc attaaaagag gacctcgtgt ttctgcgtat cgttgtaggc ttgacttgct
    13921 tcactgtagc ggcaaggtac gagccaatcg tggagaaaaa aggagttaat gtaaattatc
    13981 atgccctttt atactgaacc ttaagtgcaa gtattctttg gtcagtagga ggcattacat
    14041 caactgactt tcctcaccct ctgcagagcg aaagctaata ctcactaacg tgccgccgcc
    14101 ggaaggttgt gaaaaagtca gcgtcccacc cagccgttcc gcgcgttcgc gcatgatatt
    14161 cagaccataa tgaccttcgg gttctttcgg ttcaccgata ccaatcccgt tatcacggat
    14221 ataaaccgtg tgattgccgt ccggcgcggt gacgcaactg acggcgattt cgctggcgtt
    14281 ggcgtgcttc atcgcattca gcaccgcttc gcgaataatt tgcaacaaat gcacctgcat
    14341 ttgcgcatcc agcgcaaggg ttggcagacg gcagtcgagg gtcagtttgg cgctggtttg
    14401 attttgtaac gtatccagca tttccctcaa cgcggagggg agatccgcct gctgcagcgt
    14461 caggcggaaa gtagtcaaca gctcgcgtaa ctgccgataa gcatcattca acgcctggga
    14521 aaaatcggcc atgatacttt gtgcggtggc gttatcttcc ggtatcgaac gcttcagtaa
    14581 cgtcaactgg atacgtaagt aagaaagtac ctgagccagc gagtcgtgca attcgcgggc
    14641 gatggttgca cgttcttcca tcaacaataa atgctgaaaa tgcttctgcg cctgattaaa
    14701 gtacaaaccg cgtcccagca tcgacgaaac gctgttaagc agcggttctg atgatgaaac
    14761 gtgactattt tgccagtgca gttcgccgta aaccgtctct tgcattgtca ccggtaaaat
    14821 ctgcatcggc aattccgggt ttggttgccc ttcgctaatc cgccagtttt caccgacatt
    14881 taactccaga tattcagccg cttcattgtc gcgaacaatc tgcaaaatat ggcggaaaca
    14941 atgcacatca atctggcttg tgttcagcgc ctgcgaacat tgatacaaca cctccagacg
    15001 acgcttggcc tcgtggagat cgcgggtctt ttcttctact gacgcctcca gcgaaaggta
    15061 caatttatgc agctcgctcg acatctggtt aaaggttttt gcaagcagac caagctcatt
    15121 cggcagactg gtatccagcg gcggcgagtc gaactgcccg tgttcaatac gttgactggc
    15181 ggtaaccagc tgattcagcg gggcaaccac ctgatggcgt atacggcgca aagtaaaaaa
    15241 gaccagcgtg aaaataccga taccgccagc cagggaaatc gccaccacca gcagcatttt
    15301 gcgttcagcg tagtgctgta aagccagcac gaacaggtct atctgattaa cataattatt
    15361 aatattggcc tgataccacg gcaaatcgcc cttgctgagc cgattattca tctccagcca
    15421 gttggcattc agatgcgcat agcgagtttt tactgcttct ggcacatacc agacgttgag
    15481 attggttaat accggtgaat gcagtgcctg ctgaaacaac tggcgatgtg cattgagttg
    15541 tggactgcca ctttgcaagt cgtagcccag gcggtaactc tgcatacgca gcgatccggc
    15601 aatattgata gcctcagcgt cgcgcaaact gctcgccaga gttagcagag cgatacccgt
    15661 ggaaagaatc gacagcagca caatgtaaaa aaaggcccgg gccagactgg ccgagacggg
    15721 tcgtttaaca atcacacgcg tcttctccac aaaaaatcat taatgttcca gttcgctgaa
    15781 acgcaattta gtcagtgatg ggattatttg agctgccaca gtttatagcg aaatagttac
    15841 ttattctggg cacatgaaca ttgccgattc ccctgtaagt cgggtaataa caacactcat
    15901 ataaagaata aggtttttac aaccaaaaaa gaaggtcgtt atgaatcgtt ttattatggc
    15961 caacagtcag caatgtctgg gttgtcatgc ttgtgaaatc gcctgtgtca tggctcacaa
    16021 tgatgagcaa catgtcctga gccaacacca ttttcatccc cgaattacgg ttatcaaaca
    16081 tcagcagcaa cgtagtgcag tgacctgtca ccattgtgaa gatgcgccct gcgcccgtag
    16141 ctgccctaat ggcgcaatca gccacgttga tgacagcatt caggtcaatc agcaaaagtg
    16201 tattggctgt aaatcctgcg tggtggcctg tccgtttggt acgatgcaaa tcgtcctgac
    16261 acccgtcgcg gcaggaaaag taaaagccac ggcgcataaa tgcgaccttt gtgcagggcg
    16321 cgaaaacggt cctgcctgtg tcgagaattg cccggcggac gcgctgcaac tggtcactga
    16381 cgtcgcactc tccggcatgg cgaaatcccg ccgcttgcgc accgcacgtc aggagcatca
    16441 accgtggcat gccagtaccg ctgcacaaga aatgccggta atgagtaaag tcgaacaaat
    16501 gcaggcaacg cccgcgcgtg gcgagccgga taaactggcg attgaagcgc gcaaaaccgg
    16561 ttttgatgaa atttatctgc catttcgcgc cgaccaggca caacgggaag cctcgcgctg
    16621 ccttaagtgc ggcgagcaca gcgtttgtga atggacctgc ccgctgcata accatattcc
    16681 gcagtggatt gaactggtga aagccggaaa catcgacgcc gccgtcgagc tttctcacca
    16741 gaccaacacc ctgccggaaa ttaccggacg cgtttgtccg caagaccgtt tgtgtgaagg
    16801 tgcctgtact attcgcgatg agcacggcgc ggtaactatc ggcaacattg aacgctatat
    16861 ttcagatcag gcgttggcga aaggttggcg tcctgactta agtcatgtca ccaaagtgga
    16921 caagcgggtg gcgattatcg gggcaggtcc ggcagggctg gcctgtgcgg atgttctgac
    16981 ccgcaatggc gtggcggtga cggtgtacga tcgccatcca gaaatcggtg gcttgctcac
    17041 tttcggcatt ccttctttca aactggataa atccctgctg gcacgccgtc gggaaatctt
    17101 cagcgcgatg gggattcact tcgaactcaa ttgtgaagtg ggtaaagatg tctctttgga
    17161 ttcgcttttg gaacaatacg acgcggtctt cgttggcgta ggcacttacc gttccatgaa
    17221 agcgggttta cccaatgaag atgcgccggg cgtttatgac gcgctgccgt tcctcatcgc
    17281 caacactaaa caggtgatgg ggctcgaaga gctaccggaa gagccgttta tcaataccgc
    17341 cggacttaac gtcgtggtac tgggcggcgg cgacaccgcg atggactgtg tgcgtactgc
    17401 gctgcgccac ggcgcgagta acgtcacctg cgcttatcgt cgtgatgaag ctaacatgcc
    17461 aggctcgaag aaagaggtga agaacgcccg cgaagagggg gccaacttcg aatttaacgt
    17521 ccagccggtg gcgcttgagc tgaatgaaca aggtcacgtt tgcgggattc gtttcctgcg
    17581 cacgcgtctt ggcgagccgg atgcccaggg gcgtcggcgt ccagtaccgg tggaaggcag
    17641 tgaatttgtc atgccagccg acgcggtgat tatggcgttt ggcttcaatc cgcacgggat
    17701 gccgtggctg gagtcgcacg gtgtaacggt agacaaatgg ggccgcatca tcgcggatgt
    17761 ggaaagccag taccgttacc agaccaccaa tccgaaaatc ttcgccggtg gcgacgccgt
    17821 gcgtggtgcg gatctggtgg ttaccgcaat ggcagaagga cgtcatgcgg cacaggggat
    17881 tattgactgg ctgggggtaa aatcagtcaa atctcactga tagcctgcgc agacaaaccc
    17941 gacttcacag cgtaagataa ttgttcattt cgcgctgtgg agtcggtatg acgcaacaaa
    18001 tcaccctcat taaagacaaa attctctccg ataactattt caccctgcac aacattacct
    18061 acgatctcac gcgcaaagat ggcgaagtta tccgccataa acgtgaagtt tacgatcgcg
    18121 gtaatggtgc gacgatcctc ctgtacaacg cgaagaaaaa gaccgtggtt ctgattcgtc
    18181 agttccgtgt cgctacctgg gttaatggca atgaaagcgg gcagctgatt gaaacctgcg
    18241 ccgggctgct ggataacgac gaaccggaag tgtgcattcg taaagaagcg attgaagaga
    18301 cgggctatga agtgggcgaa gtgcgcaaat tatttgaact gtatatgtcg ccaggcggtg
    18361 tgactgagct aatccacttt tttatcgccg aatacagtga caatcagcgc gctaacgccg
    18421 gtggcggtgt cgaagatgaa gatattgaag tgctcgagct gccgttcagc caggcgctgg
    18481 agatgatcaa aaccggcgag atacgtgacg gtaagacggt gttattgctt aactatttgc
    18541 aaacgtcaca tttaatggac tgaaaaataa caataatatt tcgttgttta ttattggctc
    18601 aatccgacaa atccgattga gccgcgctac ctgcgcaacg aagatacgac ttgtgctgtt
    18661 tgtttgaact tctggggtcg taccgtccat gcgctatcgc attttccttc tctttttttt
    18721 cgctttgttg ccgacgtctt tggtgtgggc ggcaccagcg caacgggcgt tttccgactg
    18781 gcaggtcacc tgtaataacc aaaacttctg cgtggcgcgt aatacgggcg atcataatgg
    18841 actggtgatg accctgagcc gcagcgccgg ggcgcatacc gatgccgttt tacgtattga
    18901 gcgcggcgga ttgaagtcgc cggaggcgtc agaaggggag atagcgccac ggctgctgtt
    18961 agatggcgag ccgttggcgt taagtggtga caagtggcgg atttcaccat ggttattagt
    19021 aaccgatgat acggcaacca tcaccgcgtt tttgcagatg attcaggaag ggaaggcgat
    19081 caccttacgc gacggcaatc agaccatttc gctgagtggc ttaaaagcgg cgttgttgtt
    19141 tattgatgct cagcaaaagc gcgttggcag tgaaaccgcg tggatcaaga aaggggacga
    19201 accgccgctc agcgtaccgc ccgcgcctgc gctgaaagag gtcgcggtgg ttaacccaac
    19261 gccgacgcca ctctcactcg aagaacgcaa cgatttgctg gattatggaa actggcggat
    19321 gaatggtctg cgctgctcgc ttgatccatt gcgtcgtgag gtgaatgtca ctgcgctgac
    19381 tgatgataaa gcgctgatga tgattagctg tgaggcaggg gcctataaca ccattgattt
    19441 ggcatggatt gtgtcgcgta aaaatccgct ggcctcgcgc ccggttcggt tgcgtttgcc
    19501 gttcaacaac gggcaggaga cgaatgaact ggaactgatg aacgcaacat ttgatgagaa
    19561 atcgcgtgaa ctggtgacct tagcgaaagg gcgcggatta agcgattgtg gcattcaggc
    19621 gcgctggcgc tttgacggtc agcgttttcg cctggtgcgt tacgccgcag aacccacctg
    19681 cgataactgg catgggccag acgcctggcc cacgttgtgg atcacccgtt aaacaaaatg
    19741 gcggggacat gtaggccgga taagacgctg taggccggat aagacgcgtc agagtcgcat
    19801 ccggcaatca gcgtccggca atcagcatcc ggcgatcacc atcaggcacc tttcactccc
    19861 agcaccttat gcgcttttgc cacaatattc tcggcggtaa agccaaagaa cgggaacagc
    19921 ttatccgccg gagcagattc cccataacct gtcatcccaa caatcgcccc tttcaaacca
    19981 acatacttgt accagtaatc ggcaatacct gcttccaccg ccacgcgagc cgcaacgtta
    20041 gaaggcaaca ccgactcccg atattcctca tcctgggcgt cgaaaatatc agtcgagggc
    20101 agggaaacta cgcgtacatt gcgaccttct cccgctaatt tctctgccgc ttgcaggggg
    20161 atttccatct ctgaaccggt ggcaatcaaa ataatatctg gcttaccgcc gctgtctttc
    20221 agcacataac cgccacgggc aatctctttt acctgatccg gcgtacgttc cacctgggcc
    20281 agattctgcc ttgagaggat cagtgccgtc ggtccgttgt ggcgctcaac cgccagcttc
    20341 cagcccaccg ccgcttccac ctgatcgcac ggtcgccagg tgctgaaatt tggcgttaag
    20401 cgcaggctgg ccagttgctc aacagcctgg tgcgtcggac catcttcgcc caggccaatt
    20461 gagtcgtggg tataaaccat aatctgccgc gctttcatca gtgccgccat ccgcgcggcg
    20521 ttacgggcgt attcaacaaa catcaggaac gtggcggtat acggcacaaa gccgccgtgg
    20581 tgcgcgatgc cgttggctat agcggtcatg ccaaattcac gcaccccgta gtgaatgtag
    20641 ttgcccgccg gatcttcctt cagcgaaaca gaacctttcc agatggtcag gttgctggag
    20701 ccagatccgc cgaaccgccg agcaactcag gcagcatcgg cc
//
LOCUS       DAJVUJ010000062        20720 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.062, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000062 DAJVUJ010000000
VERSION     DAJVUJ010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20720
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.062"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(73..4533)
                     /gene="mukB"
                     /locus_tag="OQQ48_002089"
     CDS             complement(73..4533)
                     /gene="mukB"
                     /locus_tag="OQQ48_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HCS1952864.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLDGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVVFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVCADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAAYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLVISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGTDEAPSQAS"
     gene            complement(4533..5237)
                     /gene="mukE"
                     /locus_tag="OQQ48_002090"
     CDS             complement(4533..5237)
                     /gene="mukE"
                     /locus_tag="OQQ48_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HCS1952865.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            complement(5218..6540)
                     /gene="mukF"
                     /locus_tag="OQQ48_002091"
     CDS             complement(5218..6540)
                     /gene="mukF"
                     /locus_tag="OQQ48_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HCS1952866.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLQVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6537..7322)
                     /gene="cmoM"
                     /locus_tag="OQQ48_002092"
     CDS             complement(6537..7322)
                     /gene="cmoM"
                     /locus_tag="OQQ48_002092"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HCS1952867.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7458..8237
                     /gene="elyC"
                     /locus_tag="OQQ48_002093"
     CDS             7458..8237
                     /gene="elyC"
                     /locus_tag="OQQ48_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HCS1952868.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTAKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8214..9107)
                     /locus_tag="OQQ48_002094"
     CDS             complement(8214..9107)
                     /locus_tag="OQQ48_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HCS1952869.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIYGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9261..10007)
                     /gene="kdsB"
                     /locus_tag="OQQ48_002095"
     CDS             complement(9261..10007)
                     /gene="kdsB"
                     /locus_tag="OQQ48_002095"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HCS1952870.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10004..10186)
                     /gene="ycaR"
                     /locus_tag="OQQ48_002096"
     CDS             complement(10004..10186)
                     /gene="ycaR"
                     /locus_tag="OQQ48_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HCS1952871.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10238..11470)
                     /locus_tag="OQQ48_002097"
     CDS             complement(10238..11470)
                     /locus_tag="OQQ48_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HCS1952872.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11507..12493)
                     /gene="lpxK"
                     /locus_tag="OQQ48_002098"
     CDS             complement(11507..12493)
                     /gene="lpxK"
                     /locus_tag="OQQ48_002098"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HCS1952873.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGSKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAEQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12490..14238)
                     /gene="msbA"
                     /locus_tag="OQQ48_002099"
     CDS             complement(12490..14238)
                     /gene="msbA"
                     /locus_tag="OQQ48_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HCS1952874.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14275..16547)
                     /locus_tag="OQQ48_002100"
                     /pseudo
     CDS             complement(14275..16547)
                     /locus_tag="OQQ48_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
     gene            complement(16754..17038)
                     /gene="ihfB"
                     /locus_tag="OQQ48_002101"
     CDS             complement(16754..17038)
                     /gene="ihfB"
                     /locus_tag="OQQ48_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HCS1952875.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17198..18871)
                     /gene="rpsA"
                     /locus_tag="OQQ48_002102"
     CDS             complement(17198..18871)
                     /gene="rpsA"
                     /locus_tag="OQQ48_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCS1952876.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18982..19665)
                     /gene="cmk"
                     /locus_tag="OQQ48_002103"
     CDS             complement(18982..19665)
                     /gene="cmk"
                     /locus_tag="OQQ48_002103"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCS1952877.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VATFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(19838..20601)
                     /gene="ycaL"
                     /locus_tag="OQQ48_002104"
                     /pseudo
     CDS             complement(19838..20601)
                     /gene="ycaL"
                     /locus_tag="OQQ48_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
BASE COUNT         4988 a         5723 c         5033 g         4976 t
ORIGIN      
        1 tatgtgccat ttttttagtg cagaaacgga gttttcggaa aaagaaaagg cggcattgct
       61 gccgccttaa ttttaactcg cctgagaagg cgcttcgtca gttcctggaa gcgtttcagg
      121 gagttgcggc gcaaatcctc gcaggccgac gacatgaaca tgttcggtat tctggaagac
      181 tttacgcacc agtttatagg tggtgccttt ctccgggctg atattttccg gcgctgcgat
      241 gatgagctgc atttgcaaac gctcacacaa ttcaaacagc gtggcgatag aacgagcatc
      301 cagtcgcgct gcttcatcga ggaacagcag acggcaagga gagatatctt taccgcgcag
      361 gcggcgagat tcatcttccc agctttgtac caccatcacc agaatcgaca taccggtacc
      421 aatcgcctca ccggtcgaca atgcaccaga ctctgcgcgc aaccaaccat cggaaccacg
      481 gttaacctca acttccattt ccagatagtt gcggtaatcc agcagttctt cgccaatggt
      541 ctgcggcgtg cgctgcccca tatcaatctg cggattaaga cgctgataca atttcgccaa
      601 cgcttccgag aaggtcagac ggttgctgtt aaacagatcc tgatgctgct cgtgctgttc
      661 agagagcaca tccagcagca tggcgtgcgt ttcacgcacg ttcacgttga ggcgcacgct
      721 gttcacctga ccaaacgata cgttctgcaa cccctggttg agcatacgga tacggttctg
      781 ctcgcgctga atggttttgc gaatgatgtt cgccacgctg cgggaactga tcaccagttt
      841 ctgttcacgg gaggttaatt cttcggtcag acggctaagt tcgatctcca tctgttcgat
      901 agcttccacc ggatcatcgg tacgaataat atcctgacga atacgttcac gcagatgctg
      961 ataagccgcc acgaagaact gaattttacg ctccggacgt ttcggatctt ccgacatgcg
     1021 cagcacgtcg cgcagatgtt cgttatccgc caccgccaga cgcagcgcac ccaatgcctt
     1081 atccgacata gagcgcaaat catcagcgga gagataagcc agctcacgac ggtgtaagcg
     1141 gcgctcaacg ccgttatctt tcaccatgcg catcaccgca caccagcccg ctttggcggt
     1201 cactacctgc tcgcgcatct caaagtaatc ccgctccagc ttgcgcagtt tgcgggtcag
     1261 gttgtccatc tccgcttcgc agaaggtaag cgctttttcc agttgattgc ggcgtgaacg
     1321 gttattgctc agttgcgcat gcagctcgtc acggcgaata cgcgcccgct cttctgcccc
     1381 gctatcagca cacacgccga tatcctgcaa ttcacgttgc agatcgttga gtagctcttt
     1441 tttggtgtcg taagaacttt tcagcgaagc cagcacctgg ttgtactgac tcaactgcgc
     1501 tgcgtgaccg cgcaacgctt cgcgagcgcg ggtacgctcc gcttccgcct gttccagacg
     1561 ttcacgcagt ttttcgttga gatcgctgtt accgctaagc atttctgccg agtcagagta
     1621 gctaaagtgc gcacgacgct gcaccacttc cgtcagggca aacgcctgct gacgggcatc
     1681 gcgctgcatc tgttgagagt acgcgtaatc ttcttttaac tgttcgaact gttccgggtc
     1741 gctctgcaat accgaaacga tcggttccag tttcgccagt tgattgccaa actgctgaac
     1801 aaaacgcgcg gcttcctggg cttcatccag acgttcgcgg atttcatcga cgcgatccgc
     1861 caggctatca tcagccaaca ggttgagacg cggcagaatg cggttcagcg ccgtaacgcc
     1921 ctctttcgcc tgctcaaact gaatacgctg ctgctggtta tcattttcat gattacttaa
     1981 cgcccgctcc agttcgacgc gacggctgtt cagttgacgg atttctgctt ccgggtcaga
     2041 ctcaaacaca accgccagat gactgccgat aaagcggctg aacgcctgat gcagacgctg
     2101 agttttctgt acatcaaagg agagcgtggc gaagcgttcg gaaagcactt cacgctcggc
     2161 atggaggctt tcaatacggc tttcacgcgc agcacgacca aacagcggca cttccgggaa
     2221 acgtgaataa cgccactgac gatcggcgat tttcaccact accgcttttt ccagctcatc
     2281 aacgctgaac acgctgtcat cgaatgactg cggatctcct tcgatcagat agagatcttc
     2341 cgggcaatcg gtcaagccat ctaggtgttc agttacctgt gacagatctg gcaccacgat
     2401 ggcgtggcgt gacgggccat acagcgctga gaagtacggc gcatcttcca ggctaacgtc
     2461 gtcataaatt tctgacagca gcacaccacc aaaacgctcc gccagcgcgt tcagacgctg
     2521 atcttcagag ccgccaggct ggcttaaacg ttcgatctct tcatcgacgg cgtttttgcg
     2581 cgcgcccact tcatcgcgtt caacaatcgc ctctcgctca cgctccagca actgttgcag
     2641 atattcggtg acgtcctggc tggaggtaaa ctcttcgccg cactgttcgc tcaactggtt
     2701 gagactgttt tgcgctgcca gccaaaccgg cgcacgctgc atcaaactct gaatgcgaga
     2761 ctgcagctgt tcctgctcct ggcgcagtgc catgcgctct tcacgggcgt tagacacgct
     2821 atcggaaaga gaggcaatgc gtgcttccag ttcctgatgc agggcttcca gttcgtcgat
     2881 atcaaaattc ttgccctgac gtttgcagaa atctgccagc agacgctcag cttcttgctg
     2941 ctcgcgtaga cgctgttcca gttcgcttaa tcgcatccgc agcggctgaa cctgctctgc
     3001 caggtgacgc tgatcgaccc cttcgcgcaa tagttcgcga gcgacatccc acgcctcgtt
     3061 acgcgccagt gggccgttga ttgccaccac cagctgataa gcctgctcaa actggctgtg
     3121 cgcggtttgc gccatgctca ttttctgctc aagagagagc attttttcag tcgcttccag
     3181 ctctttcgcc tggaaggttt ccagccattc ggcggcgctg tcggcggtta agtccggcag
     3241 atggcacagt tctttggcac gattaagcgc agcaatcgcc tggttatact ggatcgcgcg
     3301 cgtttgctgg acgtccagcg cctgctggta gtcagcaagc tggcttttca gctcgtccac
     3361 ttccagttcg gcagcttccg cgcgagcctc gttctcttcc tggcgttcga tggcttctgc
     3421 caccacttcg ttttgctctt ccagacggat ctgcagctca tcgagatccg cttcgtagcg
     3481 ttcgatcttc tcctgctgac gcagtgcggt ttgcaccagg ttcaggtgat cgctggccgc
     3541 ctgataatcc gcttccagat cgccttcggc accgttgtgc tctgccagct cacgcgccat
     3601 atcgacgtgt ttgtactgct cagccgccag ttgctgacgc gaagtatgta actcgcgacg
     3661 aaactccagg gctttgtcga gatggacacg gcgctcgttg gcgtgacgca tgtagtccgc
     3721 cgccacgtag ttggtagctt cgctgatcag atgcttaaac aggtcacggt ccgactgggt
     3781 gacacgaatc gcttccagcg tcatgcggtt ttcacgcagc gccgcttcca tgtcctggaa
     3841 cgctttacgc acgccgctgt tttccggcaa cagatagtcg cgcagagaac gggtaatggc
     3901 actggagatc ccgccataca gcgaagcttc gatcagacga tagaatttgc tacggtcaga
     3961 tgcggagcgc agacgacgcg cgatgatgcc cagatcgaac atcagcgagt ggtaatcggt
     4021 aatggagttg aactgtttaa actgcacgcc ttccatcgcc tcgagcttgt ctttcagctc
     4081 gttaagcggc agcacgcgcg cctggcgttc gttcagggtt tcggtcacca gctgtgtcgg
     4141 ctgcaccgac atcggcagtc cctgaatggc aaatggtttg atatccactt tacgatcgcg
     4201 tccggcaacc tgttgcagac gcacaccgac caccacgcgc tggtggcgcg agttaatggt
     4261 gtcgagcatc gaataacaga cacccgcttt cagcttaccg tgcagacctt tatcgcgcga
     4321 accgctggtg gccccggctt ccgtagtgtt acggaaatgc agcagggtca ggtcggggat
     4381 cagcgccgta acgaacgccg ccatggtggt ggatttaccc gccccgttac cgccagaaag
     4441 cgtcgtgacc agctcgtcaa ggtcaaaagt tcgggcaaaa aagccgttcc agttaatcag
     4501 cgtcagtgag cgaaatttac cgcgttcaat cattattctt cctctccgct atctggctga
     4561 ttctcttcgg tttcatcgtt gagttgcaga tgattttcaa tcggcattgc ttcgccatca
     4621 cgaatcaggc gacgctgtgc ttcacgggga tcgtcgccag cacgcacatc ggctccgaag
     4681 cggaacaccg attcggtaat gcgaaacttg ctgctgtcgt ggcccataaa ccacaccatg
     4741 cctaaacgac gcagacggtt gagcgaagaa cgtactttct cctgcaactt ctgacggtca
     4801 acgtctgaac cggttgaacg gttgttcacc agtttcagca gttttgcttc atcggccagg
     4861 gtgagcagtt cgtcgtacag ttcctgctgg gtgaaaatcc cctcattcgc cagccgttcc
     4921 gggctgagat agagataaca gaggattttc ccgaccatca tatccagttc cgacaagacg
     4981 gaacgcggga taagagtggt ggaacgtggg cgtaaataga agaacccttc cggtgcgcga
     5041 ataagctcaa cgttataacg cgcgtaaaac tcttccagat attcctgaaa atccatcagg
     5101 aatgcatgat tatccagttc gtcgaggcca atatggcggc ctgaacgtaa ggcgctgtcc
     5161 agcgccggaa ataacggatt cgccagcgcc tgcgccagct taaccggcat cacttgttca
     5221 atatttgtcg atgacatgcg cctgtacctt ggctccgtaa tcattaatcg gctgccattt
     5281 cgctggcagt ccggtgaaat ctgcttgcgc tacgccaaga cgtaccgcct gatcaataac
     5341 aatacgcgca acgtcaaagt gacgtgcacg cggatactgt gacagatatt cgcgtaccac
     5401 cagaccaaga tccagcggca cttgtctggt tttgtacacg gcaagttgtt cttcgatgat
     5461 cgccgccagc tgttcgcgga tctcgttaaa ctcttcgtat tccagatcct caggaagttc
     5521 cccagtcact tcttcatcgc gcagtgccat ctcttcgtca cgcatatcca gcagacgatc
     5581 ggcattggca taagttagcg cccacggctc atcaaaatag gtttgtaccg actgacgtaa
     5641 ccgctgagca aagacgcggt ttttatccat atcgatcgcg gtacgaataa atttgtgtac
     5701 gtggcggtcg tagccaatcc acaagtcgat ggattgctgg ccccaactga taatacgatc
     5761 gagtttgctc tgcagatcga acaccagacg atcgacgaaa tgcagatcgt catgggtcat
     5821 cgtcgcatcc tgaatgcgca acagattagc ctgcaatttg tcgcctgccg cttccagcgt
     5881 atcctgcaat tcacgcagcg ttccggaagt ttccgaaagc aacaattcac agctggaaat
     5941 cgccgcccgc cagtctttgt tcagcaactg ggcgatatcg tccttcacct gctgctgctg
     6001 ttcgtccatc agacgttgcg tcaggtcgat actgtcgaaa atttctgcta ccgaatattt
     6061 cagtggcgca tagacattac ggtgccagtg aaattcatca ccgccctctt cggcggcatc
     6121 tgctgcgcgt ttgagctcac ccgccacaat cgacaactgc atagaaagac gcagcgtaga
     6181 aaactcgcgc tgacggatgt agtagtcagt aatgccgatg ccgagcggcg tcagacggta
     6241 aattgcgttc ccttccgcct gctcgctggt aaagcggttc agcagacgtt gacgcaccat
     6301 gtcgttgatc gcgttattgg cgcgcacgcc gatggtttcg ctggtttgct caaacgcatc
     6361 actcacatgg cggaatgcat ccaccagctc gccttcactc atctcaccat ccagacgctc
     6421 gccgttcagc gtggcaaccg ccagcagaaa agagagtcgg tctacctgca gcgagatgga
     6481 gaagtcattt tttctggccc aggcaaccag ttcggggact gtctgggaaa attcactcat
     6541 actttatcct tgctctgcgg tttgcgcgcg gtgacatgaa tataacgccc cagggtaata
     6601 tacggttcct gacggcaata acgcgtttct aattcaagta atgcttcata gcagtcgcgc
     6661 tgctggtgct tctcgcgcag ataatcatga aacacgcgaa cgcctgtctt acccataatt
     6721 tgccaaccag cttcttccag ccacagataa acctgcgccg ggtcgcgtgg ataatctggc
     6781 gaaagcgtcc gttttttctt tttcggcatt cccgcctgca cgtaatcaaa attcccggcg
     6841 accatgttat gcatcaacaa accatgcgca ttgtagaaca ttaacgacaa cacgccgcct
     6901 ggacgcaaca ctgaccacag ggtctgcaat acactgcggg gatcggccac ccactcgagc
     6961 accgcatgga acaatatcag atcaacgggc gtttccaaat gcgaagcaac atcctgagcg
     7021 gcgcaatgta taaattgcat gttgtcgctc acaccttttg cttctgccgc ctgttttgcg
     7081 cggtcgatca tctgcgcaga aagatcgcat aaaatgacct gatgcccacg ctcggccatt
     7141 ttgattgcgg tctgcccttc tccaccgcca gcatccagca cacgcagttt ttgcgggccc
     7201 atttccgcca gcacgcgatc gagatcctgc cacagaatag cctgtcgaag ctgccctttg
     7261 gtggtgccgt aaatgttacg ggaaaacttt tccgcaatat catcaaaatt gcgatcctgc
     7321 attgctttct ccaccggttg gctaagacgt tttctgataa agacgctatt ttgtcacagc
     7381 cacggcgaga atgaacccgt ctggtgtaat atcgctggcg atcccttgct atatggttaa
     7441 aaaaggaacc agaaaggatg ctttttacac tgaaaaaagt gattggcaat atgttgctgc
     7501 cacttccgct gatgttgtta attattggcg ctggcctggc gctgctgtgg tttagccgtt
     7561 ttcaaaaaac cgcgaagatt tttattagca ttgggtggct ggcactcttg ttattgagtt
     7621 tgcaaccggt agccgaccgc ctgctgcgtc ccatcgaaag cacctatccg acatggaata
     7681 attcgcaaaa agtggactat atcgtggtgc tcggaggtgg ttatacctgg aacccgcagt
     7741 gggcaccgag ctctaattta atcaataaca gtctgccacg cctgaatgaa ggtattcgct
     7801 tatggcgcga aaatccggga tcaaaactga tcttcacggg aggcgtagca aaaaccaata
     7861 cggtgagtac agcggaagta ggtgccagag ttgcgcaatc gctgggcgtg ccgcgcgagc
     7921 aaattatcac cctggatttg ccaaaagata ccgaagaaga agctgcggca gtgaaacagg
     7981 cgattggtga cgcccctttc ctgctggtga cctccgcatc acatctgccg cgggcgatga
     8041 tcttttttca gcaggaaggg ttaaatcctc taccagcccc ggcgaatcag ttggccatcg
     8101 actcgccgct taatccgtgg gaacgggcaa tcccttctcc ggtctggctg atgcatagcg
     8161 atcgtgttgg ctatgaaacg cttggccgca tctggcagtg gctgaaaggt ccgtcaggcg
     8221 agccacggca agagtgattt tgatgccaga tcgaagttgc gccgactaaa tcgtccggta
     8281 ttaaccagtt gcgcaacttc atcccataac acatacaacc aacgccgcca gatgaacgac
     8341 tccgccactg gcgcgcgttg caggtaactc cagagcaaat cttccgccag agaattatcc
     8401 attagtcgga acagttcgta ttctcgcggt gcccaaagca ttagtcccgg cccgaccatc
     8461 gccagtaact gatcgctgcg cgaatctttc agcatgctgc gtaaacagaa gttaccgtaa
     8521 atcaacacac aattgtcgtt aaagccttca aataatgccg ggagacattc gcgagtgcga
     8581 aacaggatcc gcttatcctg catcgtcaga ccggtgttat tgaactgatt gagcgtggtc
     8641 catagcactt caacatgttg ccggtaccat gagggccaga aattttcctg agtattgtcg
     8701 accgcgccga cgcaaccgcg actgtcctga cggtgccatg ccagtaaggc ttcaacgatt
     8761 tggtctttga gctgttccca gcgttctggt gttcgggctg gtgcctccac cgaaacacca
     8821 cgcatccgct ccagtagcag gacatcaggg ccggggtgtt cttcatgcgt catcacacca
     8881 taaatagtcg gcatacggac agtcccactt cttgccagca tggtggtttt ccatgccagt
     8941 tgtcgggctt ttccgggcgt actaaagctt cttgccatta acggcattgg gtttccctgg
     9001 ctgtcataca aagcccacaa cgccgtatcc gctttttcat tgacgcactc aatacgactg
     9061 agtttttcgc ccagtaaatg gcttaattcg gcacgcagct gttccattca gattaccctc
     9121 ataaaagcta ctgctttcat aatgagcgcc aatataaaag atgtcacctg aatgagatca
     9181 attgttgaaa agaaaaattg aggaaaatcg taacggtacg acactcctcc caaaattggc
     9241 tgaagtgtcg tgaagtgaaa ttagcgcatt tcagcgcgaa cgcgctcaag atcttcaggg
     9301 gtatccacac ctgtgccagg aacttcctga gcaacagcaa catggatttt ttcgccgtac
     9361 cacagaacac gaagctgctc taacatttcg atgtgttcta acggacttgc ctgccagttg
     9421 acgtaacgac ggataaagcc tgcacggtag ccataaatgc caagatgacg caggaagtta
     9481 tcgccaacgg tttcaaggcc ttctgcaaaa cgatcacgat cccaaggaat ggtggcgcgt
     9541 gagaagtaca gtgcataccc ttcagcgtcg agaaccactt tcaccgcatt cgggttaaac
     9601 gcttcttccg cattgtggat tggcaccgcc agagtcgcca tacccacctg acgctgagcg
     9661 aggttatcag caacctgacg aatgattgtc gcagggatca tcggttcatc accctgcaca
     9721 ttaacgatca ccgtgtcgtc gctgaatgcg catttttcga caacttccgc cagacgttct
     9781 gttcctgact gatgatcggc gcgcgtcata catacttcac cgccagcggc ttcaacggcg
     9841 cgggcaacat cctcatgatc ggttgccacg atgatgcgct cggcacctga ttcacgcgcg
     9901 cgttcaagaa catgaacaat catgggtttg ccgttaatat caaccaatgg tttaccgggc
     9961 agacgcgtgg acgcgtagcg cgcgggaata atgaccacaa aactcatgat ttactctcat
    10021 cagcagtcag cacgcgggct tccgtttcca gcaacactgg aatgccatcg cggagcggga
    10081 aagccaggtt atccagtttg caaataagct cttgtttttc ctggttatac caaagttttc
    10141 cgttgcaaac cgggcaggca atgatttcaa gcaaacgatg atccatagtt cctccagatg
    10201 gatcgggtta tgaatgcata aatcttatca taatcattta tgcgacgggg tctatttccc
    10261 agccagtgcg gcaatcagta aagagacctt gcgggcagcg tcctaatgtc acccgcgttg
    10321 cctgctgcca gctcgcgaaa tcagtaatcg cctgacgtaa ccctttttgc agcattgtcg
    10381 ttggtttaat gccttcctgt aaccacagag agataacttc aaggatgcct gtctggcgat
    10441 gcattttggc atccattcgc ccaactaatt gcccacgatg taataatggc agaacaaaat
    10501 agccatactg gcgtttcggc gctggggtat agcactccag ccggtagcta aaatcaaaaa
    10561 gctgctctgc gcgtttgcga tcccagacaa caggatcaaa aggcgaaagt accgcgctgt
    10621 gcgttgcagt gagctttccg gccagcgcac gctcgagtag cggcagcaaa tcagcatgca
    10681 gccaaagatt gcccaatttt tcaacatgca cagcaatgat ttgctgctgt tcagccctcg
    10741 cttcgcgcca cgccgccagt gccggacgtt tcagccgata gtaatctgcc agccactgtt
    10801 cgcggaatat tcccaggcta cgcgcactgt tatccagcat gatgatttct gcttctgttt
    10861 gcgaaacgag atcgcgctca tcatcccagt caggcatgac acggtgggtt aaatcataaa
    10921 cgcgctggaa gttgcgccgt tcaatcacca tcacctttcc ggcagtaaat aaaccttcca
    10981 gatgccgttt atgcggtttc cattcccacc agccgcttgc acctttacga ggatgctcaa
    11041 aatcggctga acgtaccggc cccttatcat gaatatgctg aattaactgt gcaatttccg
    11101 cctcatgttc ctgcatccag gcgtctttgt atttccagcc cattttttca ggtgccagca
    11161 tgcggtggcg aataagacga aagtcgctac gcggcataaa gcaggcttca tgcgcccagt
    11221 attccattaa ttcgccacgc gccagagact catccagcca ctgggcagga taatttccca
    11281 gacgactgaa aagcaccaga tatggactac gggcaacaat attgatggta tcgatttgca
    11341 gcaaggacat gcgggagatc gttgccggaa tatcctccaa cgacgctcga cggcggggtt
    11401 tgtttaacag gccttgtgcg gcaaggtgaa gattacgcgc atcagcaagg gagaggtgcg
    11461 gcagcgacat tcatgactcc atcaatcgaa cgctgccgcg gcgtaactag ttgccagaag
    11521 ccagcgaggt tagttgcgta agcagtttcg ctggttcatc acctgaaagc tgtgcgtcta
    11581 caggcaaata ccaccaattt tcttctgcaa aggcccggca tttcaccgca tctttttcag
    11641 tcattaccag cgtttgctcg gcgcttacca acgcactgac atccgcatgg ttcaaagact
    11701 gatgatcggc cagcggtaca catttttccg gttgtacgcc acacatcttc agcgtggcaa
    11761 aaaagcgcgg cggatgccca atccccgcca tcgccactac atgttcaagc tgagcaacgt
    11821 cacaacgcgt accggtacgt aaattcaccg cctgacccgg cagcagatgc atgggaattt
    11881 caccgctgcg agggacaccg ccgttgacga ttaccgcatc aaccgacttt aagcgccccg
    11941 ctcgctcacg cattggcccc gccggcaacc accagccatt gccaaagcga cgcacaccat
    12001 caataacgac aatttccaca tcacgcgcca gacggtaatg ctgtaaaccg tcgtcggtta
    12061 cgatgatctg cacatcaggg tgttgcgcca gaatggcttt taccgcatca gaacgaacgg
    12121 gagaaaccgc aacaggcgca tcagtgcgtt gataaatcaa cacaggttca tcacccgcct
    12181 gtgctgttgt ggtatctgcc gacaataaca gcggataaga ttcagcctta ctaccatatc
    12241 cccgcgatac gacccccacg cgaataccgc gctgttgcaa ctgttccacc agccagacaa
    12301 cgaccggggt ttttccgttg ccgcctgcgg tgagattacc aaccacgaca accggtacgg
    12361 gggcacgcca ggcgcgcttc agttttagtt tatagcaaag acggatcgcg ccactcacca
    12421 ggccatacaa ccaggagagt ggcagcaata gccgccacaa aggggattca ccagaccaga
    12481 ttttttcgat cattggccaa actgcatttt gtgaagttgc gcgtaaacgc cgcggtgctc
    12541 aagcaaatcg ttatgcgtac cgcgttccac aatgacacca tcctcgacga ccacgatttc
    12601 gtctgccttt tcaatggtag acaagcggtg ggcaatcacc agagaggtac ggtttttctg
    12661 caactcatcc agtgccgcct gaatcgcacg ttcggattcg gtatccagag ccgaggtagc
    12721 ttcatccaga atcagaatcg ggctatcacg cagcaaggct cgagcgatag caatacgctg
    12781 acgctgaccg ccagagagca gcacgccgtt ttcaccaatc actgtatcga gaccgttatc
    12841 catcttattg atgaagtcca tggcgtaggc catacgcgcc gcttcttcaa tttgctcacg
    12901 gctgtactgt tcagtccgtg cgtaagcaat gttgttagca accgtatcgt taaacagatg
    12961 gacattctgc gacaccagag caacctggtt acgtaacgac gccagggtat actcgcgcag
    13021 atcgtgacca tccatcagga tttcgccttc atcaatatcg taaaaacgag tgatcaggct
    13081 ggcgatggtt gatttacctg aaccagagcg tccaaccaga gcaaccgtct tccctgccgg
    13141 aattttcagg ttgatgttac gcaatgcagg tacgtcacgt cccggataag taaaggtgac
    13201 attgcggaat tccacgtcgc cagtcgcacg ctcgatcacg cgcttacctt cgtctttctc
    13261 ctgctcactg tccagaatgg taaacagcgt ctgacaagcc gccataccgc gctggaactg
    13321 ggcgttaacg ttggtcagtg atttcagcgg acgcatcagt gcaatcattg aagagaaaac
    13381 aacggtaatc gtaccagcag tcaggctatc catgacactt gggaagctcg ccgcatacag
    13441 aacaaacgcc agcgccaaag aggcgatcag ctgaatgatc ggatcagaga tggaagaggc
    13501 tgaaaccatt ttcatcccct gaagacgcat tcggttgctg actttatcaa agcgtttcgt
    13561 ttccacttcc tgaccaccga aaatcaatac ttctttgtgg cccttcagca tttgttcggc
    13621 gctggtggtc acctgcccca tggtgttctg catgttttta ctgatgttgc gaaaacgctt
    13681 cgatactacg cgaatcgcaa tcgaaacaat cggtgccagc acaatcaaaa tgatcgacag
    13741 ttgccaactg taatagaaca tcatgatgaa caggccgatg attgacgcac cttcacgcac
    13801 aaccgttatc agtgcgccgg aagaagaaga agcaacctgt tcggaatcgt aggtaatacg
    13861 tgacaacagc gtccccgttg actgtttgtc aaaaaatgaa accggcattc ccatcatgtg
    13921 accaaacagg cggcgacgca tggtcattac cacctttcct gatacccagg agatacagta
    13981 gctggagaca tagctggtga taccacgtaa aatcatcagc ccgatcacca ccagcggcat
    14041 ccacaccagc acggagcgat ctgttttacc aaagccatca tcaagaagtg gcttaaggag
    14101 cgataacatg aaggtatcgc tggctgcgtt gaggattaac gctacgcccg ccacgatcag
    14161 acccgcttta aaaggcgcaa tggttggcca cagtcggcgg aacgtctgcc acgtagagag
    14221 atctttgtcg ttatgcattc aaaaaaccag catttgttga aatagccgca tattctaccc
    14281 gttatccact ggcacgccaa accactgatg ataccaacga ggtaaaattt gctctctgag
    14341 gctgctaatc cgccagcctt gcgctgaaaa attgaccgtc acttgaccct gatgtggagt
    14401 atcaagccat tgatatcctt gctgttgata gcgatgctta actttattag agggcaatcg
    14461 ccatgcgtta tagcgttata gcgcgatgcc gatgcgagtg ccacttttcc attcactcgc
    14521 tgaattaatg gcaacgatga tgaggtatta ctgccatggt gaggtacctg aagcaatgtt
    14581 gtctgcactt gctgccagta acggcttagc atcttttgtt cagcggggac ttcaatatca
    14641 ccggttagaa gaatgctatt cgtcccgtca taaaccttaa ccacacagga atggttattt
    14701 cctttatcgt tgctagcttg taaaggccag tgcacgctga aacgcaatcc ttgccattgc
    14761 cacgcttcgc cacgcacaca gggctgatga tgttcccagt ttaacggact tctgatccat
    14821 aacatcggcc atgtgtgcaa tattgagtcc agccctcccc ggtgatccag atgttcatga
    14881 ctcagaataa cgccttccgg ttcaagatta tgccagtgga gccaggggat aatcagttgt
    14941 tgcccactat ccccttcggg ccaggccagt cctgtgtcat agagaatcgc tttgccgttt
    15001 ctggctatca ccattgccag cccttgcccg acatcaagca tgtacacctg ccactcgtca
    15061 ggtcgaggtt tttgccacag cggccagcac atcagcaagc ctacagccac acacattgct
    15121 ggcaacgttc gccaggcgtt taatcgccat accaccagta agaaccatgg ggaaaatgat
    15181 agccattgcc aacgttcagc aatgttgatc cacccttctt gcaaactctt taacccccag
    15241 aaaagtaaag ccaaagaccg gtcggcaaga aaccataacc cttgctccag gattaacggc
    15301 ccgcttaaat gcacaaccat cgcggcgagg atcaacggaa ccgtgataaa tgtcaccaag
    15361 ggaattgcta atagatttgc aataaacgag gtcagactaa tgccatgaaa tatgacgatt
    15421 tgcacaggca taagcaggag tgtgattccc agttgcaaat gaatgaggga aacaactgca
    15481 cgcaataccg gcggcagttg ccactcagga cagggaaacc actggtacca aaaaatcagt
    15541 gccgcgaccg cagcggcaga gagccataaa ctttgcgaga gaatggcaac aggatccatc
    15601 agcaaaattg ccgccagaca acatatccat acatcccagc cactccactg tcgcccactt
    15661 aacttaagca ttccccacgt agcaagcgcc accacggtac gcaatgcagg aggttgcatt
    15721 cccgtcaacc aggcataaaa agcagcacag cagattccac caattaatgg catttgccag
    15781 tggatccagc gcccaggcag aaaaacttgc ccaccgcgaa tgagtccggc agccagcaac
    15841 gccgcaaaag cgatgtgcaa tcccgatatc gccattaaat gcgccgttcc agtatcacgc
    15901 atgatatttt tgatttcttt ggggacggat aaccgttccc ccatacctaa accaagaata
    15961 accgcattcc acatataggg ttgcagcgtt gtttgtagtg acgccagata ctgtgcacgc
    16021 aggctacaat tcggttcaat gacacttgcc tgcagaaagc ggccgatgag cggctgatgc
    16081 tgggcaatgg cataacgctg gctatcaaag ccgccatcat taagttggcc atgaactgca
    16141 cgaactttga gtttcattga ccattgttgt ccagcacaaa ccgcttgcgg aagatattcg
    16201 ccatacagca caagacctgg cgcagggaat atacgtcgac cttgtagatg agtaatttga
    16261 ccgtaatgag tggtcatgcc gtcagttgca gtgatctcaa caattgcatc ctgcgtcgcg
    16321 ccagttaagg tttctcctgc ccacaaaatt tgctttgctg ataatatgcc ccaaacgaaa
    16381 aacagcaacg tcagcgcgat ataacggacg gttttaacag ggataaatgc cagtacgcag
    16441 gcgaagagag tcagaaacgc aagggttaat gtcccaggca attggggcaa aatcagcaac
    16501 ggaaaaattc cgcaaattat gcatacgccg actgtcgtta ttttcatgca cccctccctg
    16561 tttatggaaa gtgtgcaact ttgttgagcg gttgtctggt gggaattttg cgttccccga
    16621 ggcacattca gaagaattta acgaaagggt gaaaactgct caaaactctg cgaggcagct
    16681 tccagttcct gattagagaa atgaaaaaag cacccgacag gtgcttttct cgcgttcaag
    16741 tttgagtaaa aacttaaccg taaatattgg cgcgatcgcg cagttcttta ccaggtttaa
    16801 agtgaggaac gtattttcct tccagttcta ctttatcgcc agtcttcgga ttacgtccgg
    16861 tacgtggtgc gcggtagtgc aaagagaaac tgccgaaacc gcggatttca atacgctcgc
    16921 cctgcgcaag agtcgaggcc atatgctcca gcatctcttt tactgcatct tcaaccgtct
    16981 tggcgggaat gtgcgattgc tgggtggcaa gtctttctat caattctgac ttggtcatga
    17041 ttcctccggt tccttaaagg caaattaagc ggctgctgtt gctgcattga ttaagggcgg
    17101 ccgtagccgc ccttagtgct tgattacagg acgaaacctg caatctgtca agtaaactca
    17161 acaaacttcg gaataaaaat cccgaagagt cagagaatta ctcgccttta gctgctttga
    17221 aagcttcagc cattgcgttg ttggagaagt ttgcatcttc ctgtttgtta acagttgcga
    17281 ttgcatcttt ctcgtcagct tcgtctttcg cacgaacaga caggctgatt gcgcggtttt
    17341 tacgatcaac gccggtgaat ttagcttcaa cttcgtcgcc aacgctcaga accagggtag
    17401 cgtcttcaac gcggtcacgg gatgcttcag aagcacgcag gtaaccttca acgccgtcag
    17461 ccagttctac ggttgcgcct ttagcgtcaa ctgcagttac tttaccggtt acgatagcgc
    17521 ctttcttgtt cagagcaacc cagttgttga acggatcttc tgcgagctgt ttaacgccca
    17581 aggagatacg ttcacgttct gcgtcaacct gcagaacaac tgcagcgatt tcgtcgcctt
    17641 ttttgtattc acgaactgct tcttcgcctg caacgttcca ggagatgtca gacaggtgaa
    17701 ccaggccgtc gatgccgccg tctaggccga tgaagatacc gaagtcagtg atagacttga
    17761 ttttaccttc aacacggtcg cccttgttgt gggtttccgc gaactgctgc cacgggttag
    17821 ctttgcactg tttcagaccc agggagatac gacgacgttc ttcgtcgata tccagaacca
    17881 taacttccac tacatctcca acgttaacaa ctttggacgg gtggatgttt ttgttggtcc
    17941 aatccatttc ggaaacgtgt accaggcctt caacgccttc ttcgatttca acgaagcagc
    18001 cgtagtcggt caggttggtc acgcgaccag tcagtttggt accttccgga taacgtttag
    18061 cgatagctac ccacggatct tcgcccaact gtttcaggcc cagggataca cgggtacgtt
    18121 cgcggtcgaa cttcagcact ttaacagtga tttcgtcgcc cacgttgacg atttcgctcg
    18181 gatgcttaac gcgtttccag gccatgtcag tgatgtgcag caggccgtca acgccgccca
    18241 gatcaacgaa tgcaccgtag tcagtgaggt tcttaacgat acctttaact tccatgcctt
    18301 cctgcaggtt ttccagcagc tgatcgcgct ctgcgctgtt ttcggattcg ataacggcac
    18361 gacgagaaac aacaacgttg ttgcgcttct gatccagctt gattacttta aattcaagct
    18421 ctttgccttc caggtgcaga gtgtcacgca ccggacgaac gtctaccaga gaacctggca
    18481 ggaacgcacg aataccgttc agctcaacag tgaagccgcc cttaactttg ccgttgataa
    18541 caccggtaac agtttcagca tcttcgtaag ctttttccag cgtgatccag gcttcgtgac
    18601 gtttagcttt ctcacgggac agcagagttt caccgaagcc gtcttctact gcgtccagag
    18661 caacgtcaac ttcgtcacct acctggattt ccagctcgcc ctgggcgttt ttgaactgct
    18721 cagccgggat ggcggactca gatttcagac cagcgtcaac cagtactacg tctttgtcga
    18781 tagcaacaac aacgccacga acgatagaac ccgggcgggt ttcgatttct tttaaggact
    18841 cttcaaagag ttgagcaaaa gattcagtca tgtttaatct tcaggtttat atttaacgtc
    18901 cacctggctc catgccggat ggggttgttt aacatacccg ctgtcattcc attgcaacgg
    18961 gggtactgca aattcggtcg cttatgcgag agccaatttc tggcgcgcgt attgtagcgc
    19021 tttttcaatc acttgctcaa tgcttaaggt ggtggaatcc aacactaaag catcggctgc
    19081 cggaaccagt ggcgctaccg ctcggttacg atcgcggtcg tcgcgttctt tgatctcggc
    19141 caaaaggcgc tcaaagttaa cactaaagcc cttctcctgc aactgtagca tgcggcgatg
    19201 cgcacgttct tccgaggagg cgtcaaggaa aattttcact ggtgcatcag ggaataccac
    19261 cgttcccatg tcgcggccat cggcaatcag acctggtaat tcgcgaaacg cgcgttggcg
    19321 acgcaataat gcttcacgaa cgcgtgggaa tgtcgcgact tgtgaagccg cattcgccac
    19381 ttcctgagta cgaatttcgc cgctgacatc ttccccttcg aggatcactt ccagattgcc
    19441 attggttgac acaaaacgta catccagatg ggatgccagc ggtaccagcg catcttctga
    19501 cgcaacatca acatggtgat gtaatgccgc caatgccagt acgcgataaa ttgcacccga
    19561 atccagcaga tgccattgca atgcttccgc catagcctta cacaaggtgc ctttccctgc
    19621 accgcttggg ccatcaatgg taataaccgg ggcaattgcc gtcatcttta tctccttaaa
    19681 acaggcgtac cgttaacata accgcgcgca ttatacgcgc cgacgccaca aatcgttaac
    19741 gttgtgtgca aaaaccagtt ttgtctgtat tcagatgggg atttattgcg caattatagc
    19801 gggctggcag aagaccagcc cgaaagatga caatgattta cttaccagag gcgatacgat
    19861 cacggatgtg ttgcgcacgc tctgttgatg gtgggtgaga gtcaaacatg gactgggtgc
    19921 gaccgccatc cagactagcc agtttttcaa agctgccaac cagcccctag gtgctgatgc
    19981 cacgtttctt caacagatca aaggagaaat catctgcttc ggactcttta tcacgggagt
    20041 atttagcatt aatagcgcct tctgcgatat cacccaattg tgagcgggaa agctgggaag
    20101 ccacaccgct ggtagctgaa atggcatcgc gtgcggcaac gatcgcataa gaaactttca
    20161 tttcagccag cgagtgaccc aacgcgacgt ggcccagttc atggcccaga acaccttcaa
    20221 tttcgttgtc attcatcatg tccatcaggc cactgtagac acggacacag ccgttcgcca
    20281 tcgcccatgc gttgacgtcg ctggtcatat aaacctatag ttgaccggcg tgccattaat
    20341 attgttaccc aatgctttgg cgatttttgc cagacgttta ccgtacttgc tggatttact
    20401 tgcgacttga ttgccgctgt ccatttcggc acagccctga ttggcaatcg cttttgcatc
    20461 ggcatcgctt aatgttgctg ctttataggc atttaaaccg gagctgatag ccatattggt
    20521 atcaataccg tgggtatttt gacaccctgt cagtaatgct gcagaggtcg caatcgccag
    20581 cagtaattta gtattcttca tgtgaatcat ccgttgatta atcatatata aacattatgt
    20641 atcaacaact tacattgaca agcgacgcta ccattaatca aaatgactca caaggtccga
    20701 aaaaaaacgc cgacaatgtg
//
LOCUS       DAJVUJ010000063        20600 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.063, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000063 DAJVUJ010000000
VERSION     DAJVUJ010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20600
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.063"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(56..481)
                     /gene="nudI"
                     /locus_tag="OQQ48_002105"
     CDS             complement(56..481)
                     /gene="nudI"
                     /locus_tag="OQQ48_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HCS1952878.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVEYGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYTWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            760..1302
                     /locus_tag="OQQ48_002106"
     CDS             760..1302
                     /locus_tag="OQQ48_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1952879.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            1402..2604
                     /locus_tag="OQQ48_002107"
                     /pseudo
     CDS             1402..2604
                     /locus_tag="OQQ48_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
     gene            2824..3606
                     /locus_tag="OQQ48_002108"
     CDS             2824..3606
                     /locus_tag="OQQ48_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HCS1952880.1"
                     /translation="MLESSKVPTLTRAIDILNLIARIGPCSAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTRLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLSWQPAAVQQSIIEGL
                     VWEQATPTTITHPQQLHEELARIRRQGWSYDNGEDYPDVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKSIACARDISRLLGWKSPFDLQAS"
     gene            3621..4826
                     /gene="rhmD"
                     /locus_tag="OQQ48_002109"
     CDS             3621..4826
                     /gene="rhmD"
                     /locus_tag="OQQ48_002109"
                     /EC_number="4.2.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
                     /protein_id="HCS1952881.1"
                     /translation="MTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATP
                     MSKYRDYEQSRQSFGINVLGTLVVEVEAENGQTGFAVSTAGEMGCFIVEKHLNRFIEG
                     KCVSDIKLIHDQMLNATLYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAV
                     RDEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIRKDAAMVADMREKCGEDFW
                     LMLDCWMSQDVNYATKLAHACAPYNLKWIEECLPPQQYEGYRELKRNAPVGMMVTSGE
                     HHGTLQSFRTLSETGIDIMQPDVGWCGGLTTLVEIAAIAKSRGQLVVPHGSSVYSHHA
                     VITFTNTPFSEFLMTSPDCSTMRPQFDPILLNEPVPVNGRIHKSVLDKPGFGVELNRD
                     CNLKRPYSH"
     gene            4883..6172
                     /locus_tag="OQQ48_002110"
     CDS             4883..6172
                     /locus_tag="OQQ48_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1952882.1"
                     /translation="MSTALLDAVVKKNRVRLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGHPGWFWMFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKQEKTLLINQ
                     LASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGFT
                     ASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVMAIVA
                     LCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFVNLFGAVGGFIAPILRVKAETLFA
                     SDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            6190..6993
                     /gene="yfaU"
                     /locus_tag="OQQ48_002111"
     CDS             6190..6993
                     /gene="yfaU"
                     /locus_tag="OQQ48_002111"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HCS1952883.1"
                     /translation="MNALLTNPFKERLRKGEVQIGLWLSSTTSYMAEIAATSGYDWLL
                     IDGEHAPNTIQDLYHQLQAVAPYASQPVIRPVEGSKSLIKQVLDIGAQTLLIPMVDTA
                     DQARQVSSATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDN
                     LDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAV
                     APDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(7034..7936)
                     /locus_tag="OQQ48_002112"
     CDS             complement(7034..7936)
                     /locus_tag="OQQ48_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HCS1952884.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEIAERSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGIDRDQVLAATQLSEADLAANNH"
     gene            complement(8129..9319)
                     /gene="glpC"
                     /locus_tag="OQQ48_002113"
     CDS             complement(8129..9319)
                     /gene="glpC"
                     /locus_tag="OQQ48_002113"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HCS1952885.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(9316..10575)
                     /gene="glpB"
                     /locus_tag="OQQ48_002114"
     CDS             complement(9316..10575)
                     /gene="glpB"
                     /locus_tag="OQQ48_002114"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HCS1952886.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRSTWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLH
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLLCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPHFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(10565..12193)
                     /gene="glpA"
                     /locus_tag="OQQ48_002115"
     CDS             complement(10565..12193)
                     /gene="glpA"
                     /locus_tag="OQQ48_002115"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HCS1952887.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            12466..13824
                     /gene="glpT"
                     /locus_tag="OQQ48_002116"
     CDS             12466..13824
                     /gene="glpT"
                     /locus_tag="OQQ48_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HCS1952888.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            13829..14905
                     /gene="glpQ"
                     /locus_tag="OQQ48_002117"
     CDS             13829..14905
                     /gene="glpQ"
                     /locus_tag="OQQ48_002117"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCS1952889.1"
                     /translation="MKLTLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(14947..15189)
                     /locus_tag="OQQ48_002118"
                     /pseudo
     CDS             complement(14947..15189)
                     /locus_tag="OQQ48_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            15368..16018
                     /gene="inaA"
                     /locus_tag="OQQ48_002119"
                     /pseudo
     CDS             15368..16018
                     /gene="inaA"
                     /locus_tag="OQQ48_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416740.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
     gene            complement(16072..16326)
                     /gene="yfaE"
                     /locus_tag="OQQ48_002120"
     CDS             complement(16072..16326)
                     /gene="yfaE"
                     /locus_tag="OQQ48_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HCS1952890.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(16326..17456)
                     /gene="nrdB"
                     /locus_tag="OQQ48_002121"
     CDS             complement(16326..17456)
                     /gene="nrdB"
                     /locus_tag="OQQ48_002121"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HCS1952891.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDSEVDTDDLSNFQL"
     gene            complement(17603..19888)
                     /gene="nrdA"
                     /locus_tag="OQQ48_002122"
     CDS             complement(17603..19888)
                     /gene="nrdA"
                     /locus_tag="OQQ48_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HCS1952892.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINNLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGECP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
BASE COUNT         4846 a         4991 c         5523 g         5240 t
ORIGIN      
        1 aattcgttaa ccactaaatt ttcaatctga cagccagtga tggctgtcat cattgttaaa
       61 gaagaccttt caaacgtaac gtttttcggg tggcaacatt caaatcataa tgcaccagat
      121 cttcaggttt tacccacgtg tagtcctgaa actcttcgtt tattttcact tctcggttgg
      181 cagaaacgca gtcaaaaatc aggtaaatca tataaatctc ttccttgcga ccatctgcat
      241 acgtcttggt gcgaatatca tcgctgaagg tccacggcgt gatttctgtc aaaagcagct
      301 gttctcccag ttcttcgcga atttcgcggc gtagtgcctc ttcaattcgt tcgccatact
      361 ccacgccgcc acccgaaagc gcccattgac cggggaaaac gccgcgatcg tcggccattt
      421 tgcacagcag ataagcacca tcattttgaa tcaaagggca tacaatagtc cgttgtcgca
      481 caggggtatt ccttataaat aaaaagaaac catgagaata cggtaactca gaaatatgtg
      541 aaagtgacag taatctcttt tgcgatgata actgcactta ttattatttt tgtgtgtatc
      601 ggtaaagtgt gtttattttt tcggcgttta attatttaag aagtatttag ttacaataag
      661 tgactagatc ccttttttcg ctcattgaat cctctgtgcc aggcagtaca atcccgcagc
      721 tttgggaatt ttgattaatt gcttgtggga aaggggataa tgaaaaaaat tgcgctagca
      781 ggtctggccg gaatgctgtt ggtttctgca tcggtcaatg caatgagcat cagcggtcag
      841 gcgggtaaag aatacaccaa tattggtgtc ggttttggga ctgaatcgac gggcctggcg
      901 ttaagcggta actggacaca taacgacgac gacggtgacg tcgctggcgt ggggctgggg
      961 ttgaatctgc ctctagggcc gttaatggcg accgttggcg gaaaaggcgt ttacaccaac
     1021 ccgaattacg gcgatgaagg ttatgccgca gcggtaggag gtggtttgca gtggaaaatt
     1081 ggcaacagct tccgtttgtt tggcgagtat tactactctc cggattcact ctccagcggt
     1141 attcaaagtt atgaggaagc gaatgctggc gcgcgttaca ccattatgcg tccagtcagt
     1201 attgaggcgg gttatcgcta cctgaatttg tcgggtaaag atggtaaccg cgacaacgcc
     1261 gtggctgacg gcccgtatgt tggggttaac gccagtttct gatcccttcc ggcgcggcat
     1321 tctgtcgcgc cattcagttt cttactacgc cttatctccc tttgcgttat agtgattcga
     1381 taacaaaagc aggagataac catgttaaaa gtggaaatgt tatccaccgg ggatgaagtg
     1441 ttacacgggc aaatcgttga cactaacgct gcctggctgg ccgatttttt ctttcatcag
     1501 gggctgccat tatctcgcct caatacggtg ggggataacc ttgatgactt agtcaccatt
     1561 cttcgcgaac gtagtcagca cgccgatgtg ctgatcgtta acggcgggct gggaccgacc
     1621 agcgatgatt taagcgcact cgccgctgcg acagcaaaag gtgaaggcct ggtgctgcat
     1681 gaagcctggc tcaaagagat ggaacgctat ttccacgaac gtggacgagt aatggcaccg
     1741 agcaaccgta aacaagcgga gctgcctgcc agtgctgaat ttatcaataa cccggtaggc
     1801 accgcctgtg gttttgccgt gcagcttaat cgttgcctga tgttctttac tcccggcgta
     1861 ccgtcagaat ttaaggtgat ggtcgagcac gaaatcctgc cgcgcctgcg cgagcgtttt
     1921 tctttaccac agccgccggt ttgtctgcgt ttaactacct ttggtcgttc ggaaagcgat
     1981 ctggcacaaa gcctggacac tctacaactg ccgccgggcg taacaatggg ctatcgctct
     2041 tcaatgccga ttatcgaact gaaactcacc ggaccggcaa gcgagcaaca ggcgatggaa
     2101 aaactgtggc tggacgttaa acgtgttgcc ggacagagcg tgattttcga aggcaccgaa
     2161 ggactgcccg tgcagatcag tcgcgaattg caaaaccgcc agttcagcct gacgttgagc
     2221 gagtaattca ccggtggttt attggctttg caactttctc gcgcaggtgc tccattgctg
     2281 gcgtgtgaag tggttccttc acaggaagaa aacctggcgc aaactgcgca ctggattaca
     2341 gaacggcggg ccaaccattt tgccgggctg gcactggctg tttcgggttt cgagaacgaa
     2401 catctcaact ttgcgctagc cacaccagac ggcactttcg ctctgcgtgt gcgtttcagc
     2461 actacgcgct acagcctggc tatccgtcag gaagtgtgcg caatgatggc actgaatatg
     2521 ctgcgccgtt ggttaaacgg ccaggatatc gccagtgagc atggctggat tgaggttatt
     2581 gagtccatga ccttatctgt ctgattccgc cactctgtgc cggtttactg caccggcaca
     2641 ttccccctcc ggcaaaactt gtccaacctg ttgcactcaa taagaaaaaa tccgtgcact
     2701 gattcacaaa tttgtgcgta atttctccag tccgctggac gaggagcggg atttgatgcg
     2761 cttcacaatt ttactccccc tgagcaacga gactgattcc agtatctaag actggagtcc
     2821 agtatgttgg aatcaagcaa agttcccacc ctgacacggg ctattgatat tctaaatttg
     2881 attgcccgca ttggcccctg tagtgctgcg acgatcattg acacattggg aatccctaaa
     2941 agcacggcct atttgctgct taatgaactc aggcgtcagc gttttcttag ccttgatcac
     3001 caggaaaatt tttgtttgtg gaccaggctg gtggagcttt ctggtcatgc gttaagcaag
     3061 atggatctcc gggaactggc tcgaccgcgt ttgacgcaac tgatggatac caccgggctg
     3121 ttatgtcatc tggggattat tgataacggc agcgcttatt acattttaaa agtggagtca
     3181 tcagccacta tcagcgtgcg ttcccatgaa ggaaaaagcc tttcgcttta tcgctccgga
     3241 ataggtaaat gcctgctttc ctggcaacct gcagcggtac agcaaagtat tatcgaaggg
     3301 ctggtctggg agcaggcgac acccacaacg attactcatc cacagcaact tcacgaagaa
     3361 ctggcgcgta ttcgccgtca gggctggagc tatgacaacg gcgaagatta ccccgatgtc
     3421 cgttgtgtcg ctgcgccggt gtttaacgcc aataacgagc ttaccgctgc gatctcggtg
     3481 gtcggtaccc gcttacaaat caatgaagaa taccgtgatt atctggcggg caagtccatt
     3541 gcctgcgcca gggatatttc acgtctgttg ggatggaaaa gtccctttga cttacaagcc
     3601 tcataacaat ggagaacatc atgaccctac caaaaattaa acaggttcgc gcctggttta
     3661 ctggcggtgc gacagcagaa aaaggcgctg gcggcggtga ttatcacgac cagggggcga
     3721 atcactggat agacgatcat attgccaccc cgatgagtaa ataccgcgat tacgagcagt
     3781 cacgccagtc atttggcatt aacgttcttg gcacgttggt tgttgaagtc gaagcagaaa
     3841 acggccagac cggattcgct gtttcgacag ccggtgaaat gggctgtttt atcgtcgaaa
     3901 aacaccttaa ccgtttcatt gagggtaaat gtgtcagtga tatcaaactg atccacgatc
     3961 aaatgctcaa tgccaccctg tattactccg gctctggtgg cttggtgatg aatacgattt
     4021 cctgtgtcga tctggctctg tgggatctgt tcggcaaagt ggtcgggctt ccggtttata
     4081 aacttttagg cggcgctgtt cgtgatgaga ttcagttcta cgccacaggt gcgcgtccgg
     4141 atctggcaaa agagatgggc tttatcggtg gcaaaatgcc gacgcactgg gggccacatg
     4201 atggcgatgc ggggatccgc aaagatgccg ctatggtcgc ggatatgcgt gaaaaatgcg
     4261 gtgaagattt ctggttaatg ctcgactgct ggatgagtca ggacgtgaac tatgcgacca
     4321 aactagccca cgcttgcgcg ccctataacc tgaaatggat cgaagagtgc ctgccgccac
     4381 agcagtatga aggttatcgc gaactgaaac gcaacgcgcc agtcggaatg atggtcacca
     4441 gcggtgagca ccacggcaca ctgcaatctt ttcgtacgct ttcagaaacc ggtatcgaca
     4501 ttatgcagcc ggatgttggc tggtgcggtg gtttaaccac gctggtggaa attgccgcaa
     4561 tcgccaaatc cagggggcaa ctggtggtgc cgcacggttc gtctgtttac tctcaccatg
     4621 cggtgatcac cttcaccaat acgccattca gcgaattcct gatgaccagc ccggattgtt
     4681 caacgatgcg tccgcagttt gacccgattc tgctcaatga gccggttccg gtgaatggtc
     4741 gtattcataa atcagtgctt gataaacccg gttttggcgt cgaactcaat cgtgactgca
     4801 atttgaaacg cccctacagc cactaatcac tccgggcgtt gctgtgacaa cggtaacgcc
     4861 cacatattct aaggagcttt ttatgagcac cgctttgctt gacgccgtgg tgaagaaaaa
     4921 ccgtgtgcgt ttaattccgt ttatgttggc gctgtatgtg ctggcgtttc tcgaccgttc
     4981 gaatatcggt tttgccaaac agacctacca gattgatacc gggttgagta atgaagctta
     5041 tgcgctggga gcaggcattt tctttgtggt atatgcgttt ctgggggttc cggcgaatct
     5101 tttgatgcgc aaactggggg ccagaacctg gattggtacg acaacactgc tgtggggatt
     5161 tctttcggca gccatggcat gggccgatac tgaagcgaaa tttctgatag ttcgcactct
     5221 gcttggtgct gcggaggccg gatttttccc tggtatgatt tatctcacct cgcaatggtt
     5281 tccgcagcgt aatcgcgcca gcattatggg gctgttctat atgggcgcac cgctggcgtt
     5341 aacactggga tcaccgcttt ccggcgcgct gttggagatg catggattta tggggcatcc
     5401 cggctggttc tggatgtttg tgattgaagg attgttggca gtcggtgctg gggtattcac
     5461 attcttttgg cttgatgaca caccggagca ggcacgtttt ctgagtaaac aagaaaaaac
     5521 gttgcttatc aatcaactgg caagtgaaga acaacagaaa gtgacttctc ggctgagcga
     5581 tgcgctgcgt aatggccgag tctggcaact agcgattatc tacctgacca ttcaggtggc
     5641 ggtttacgga ttaattttct tcctgccgac ccaggttgcg gcattgctgg gaacaaaagt
     5701 gggctttaca gcgtcggtgg tcaccgccat tccgtgggtt gcggccttgt ttgggacctg
     5761 gcttattccg cgctattccg ataaaaccgg cgaacggcgt aatgtcgcag cgctgacatt
     5821 actggcggcg ggcattggta ttggtctgtc cgggctgctt tctccagtaa tggcgatcgt
     5881 agcgctgtgt gttgcagcta tcgggtttat tgccgtgcag ccggtgttct ggacgatgcc
     5941 gacacaactg ctttccggta cggcgctggc tgcggggatt ggttttgtaa acctgtttgg
     6001 tgcagtgggc gggtttattg ccccgatcct gcgcgtgaaa gcagaaacgt tatttgccag
     6061 cgatgcggca ggattactga cactggcagc ggtggcggtc atcggttcgc tgattatttt
     6121 cactctgcgt gtaaatcgta ctgttgcgca gaccgacgtg gcacatcatt aaataggtta
     6181 aggaacacga tgaacgcatt attaaccaat ccctttaaag aacgtttacg caagggcgaa
     6241 gtgcaaattg gtctgtggtt aagctcgact acttcttata tggcagaaat tgccgctact
     6301 tccggttatg actggttgct gattgacggg gagcacgcgc caaacaccat tcaggatctt
     6361 tatcaccagc tacaggcggt agcgccctat gccagccaac ccgtgatccg tccggtggaa
     6421 ggcagtaaat cgctgattaa acaagttctg gatattggcg cgcaaactct attgatcccg
     6481 atggtcgata ctgccgatca ggctcgtcag gtgtcgtctg ccacgcgcta tcctccctac
     6541 ggtgagcgtg gtgttggggc cagtgtggca cgggctgcgc gctggggacg cattgagaat
     6601 tacatggcgc aagtcaacga ttcgctttgt ctgttagtgc aggtggaaag taaaacggca
     6661 ctggataacc tggacgagat cctcgacgtc gaagggattg atggcgtgtt tattggacca
     6721 gcggatcttt ctgcgtcgtt gggctacccg gataacgccg ggcacccgga agtgcagcga
     6781 attattgaaa ccagcattcg gcggatccgt gctgcgggta aagcggctgg ttttctggct
     6841 gtggctcctg atatggcgca gcaatgcctg gcgtggggag cgaactttgt cgctgttggc
     6901 gttgacacga tgctctacag cgatgccctg gatcaacgac tggcgatgtt taaatcaggc
     6961 aaaaatgggc cacgcataaa aggtagttat tgatatcaaa ggcccatggg gatcggctgt
     7021 gggcctgtgt taattagtgg ttattcgctg ccagatcggc ttcgcttagc tgggtggccg
     7081 cgagcacctg gtcacgatca atgccctgtt ccagcatgcg caaagcaatt ttaatggctt
     7141 gttcatgcat accttcctgc cagccaattt gatgcccttc ctgccgtagc cgttcagcaa
     7201 tagtcattaa tctctccttt tgtagtggtg aacgttcggc aatctcctca ataaaacggg
     7261 tgaaacggga ggtatcgccg cattgcagca gataattaaa cagtgtttgt agctggctgt
     7321 cattagtgaa gcctctaacc aaaagcgtgg tgatcctgtc gaccatgccg attaaatcgc
     7381 ggtcgcgaat atgcttttga atcagttcca gcagagctat acgccgatgt tgcatgatct
     7441 catcgtcagg tacgatggtg atatccacca acggaaaagc ttcggtgtac aactgccggg
     7501 caagttgcgg atcgtcaaac tcatccagcc agttaagtga gtacgggtag ggcgatgttt
     7561 cgccatgata aaacagcaat ggcaccacca gcggaactct gtcatagcct ttatccaggt
     7621 gacgctgcat ggcggcagtg gcatagcgca ttagccgaaa agccatattc ttttctgcag
     7681 agctttgatg ttcaatcacg cagtagatat agccgtcacc gtcgctggtt tccacggacc
     7741 acaaaacatc cgagtagtaa gcgcgtaaac ttttttcaat aaaactagtg ggttccaggc
     7801 gtaaggtttg caggttgcaa agcttgcgca gtggttctgg taaatgtatt tcgagaaaat
     7861 cccgtgcggt ttcgggtgtg aacataaagg ttttaaatac cgcatcatgc ggggaggagg
     7921 ttgttgattc ggtcatggtt gtccgtcacc tgatatttat gatgacgcga cattacggtt
     7981 atcctaggcc tttgacatct gccagtttca gcctttgcga gacgttttca gaggaatttt
     8041 cactgcgttg caacgcatac attgggcacg gaatcgtgta cgcaaaaatg cccggtaacc
     8101 acatgcttac cgggcatcag aaaggagttt aagccagcgc ctgggccagt agcgtaatcg
     8161 gatgttcgca gcgaagactt gtggacatct caatctgcca tttacaggtt tcgcagtcgg
     8221 tgaccaccag atctgcgccg ctttcttcta tctggcggaa cagtggtgcg ccgatggctt
     8281 gtgaggtggg gtagttctct tttttgaaac cgtaagtacc cgcaataccg cagcactggg
     8341 aatccagcac cgttaactca agccccggga ttttacgcaa cagctccagg gtgtagagcg
     8401 tccagcccat tttttccata tggcacggag tgtgataaac cactttcagc ggcagcggtt
     8461 tcagcggtaa cgttttgcct tcgtccagct tgcgccacag ccagcgggtt gccagttcga
     8521 tatgatcgcg caagcctttg ttgtcgacat tcagcacttc cgggtattcg tcgcgcaggg
     8581 caaatgtaca ggttgaggag gtggcaatca ccggaatgcc ttttactccc acagcttcgc
     8641 ggatcgactc tacattcgta attgcctgtt tgcgtgcttt atcggtaaag ccgttggcga
     8701 ttagcggtac gccgcagcat ttttctttgc tgagcagttg tacaccggta cccattgcgt
     8761 tgagcacttt aattaaatct ttacctaact gcggatggtt gtagttaacg aagcagccgt
     8821 gaaagaaagc gacctggtct ttatattgtg cttgctgagc cgctatgctg cgataccagc
     8881 gacggaacgt gccgaaggag tatttcggta gcgtgcgacg atgatcgatt tttaacgccg
     8941 catcaagcag ctgccgcacc ggtttcagcg aggtagcggt gttgacgatt ggtgcgaacg
     9001 gcgtggaaac gctacccatc aggtcggtat gactcaacac aaaattacgc agcgacgggc
     9061 gcgtggtgtc atattttgcc cgcgcgcgct ggataatatc gccaatcttc acatcggacg
     9121 ggcaggcgac ttcacaacgt ttgcagttga tgcaatattt cagcgcctcg tcatacagtg
     9181 cgccatcttt caaacgcaga cgctcgccat ccggcccggc ttgttttggc cctggataac
     9241 cgggattcac ccggctcacc gggcaggcgg tggtgcagac ggtgcactta atgcagtttt
     9301 cgaagctggt gtcgttcatt gttggcctcc tgcgcgttgg gcaatctgtt gagcggcatg
     9361 taaagcactg acggcacaaa caccgccgcc gcatccctgg gcgatgggat caaatccgcc
     9421 cagcaccgaa ccgatggcaa acaggttctc aatggtttgc cctgcctgcg acgggcgtag
     9481 cgtctcatca gtggttacac cgaactgctg ccacggttgc ggcgcaaaaa aatcgccctt
     9541 ataccattca ccccgcgtgg cggtttgtag cacatcaagg ccgagaatcg gctcgcgaat
     9601 gccgttacgt tccgctacca gtccgccact aaagaaactg ccgctggcga gaaccgcgaa
     9661 atgtggacgt agcggaatat cggcgtgatt gcgggtccag atttcattca ctacgccatt
     9721 tttacaggtc acttttttca cttcatcgcc cggcatccac acgccaccct ggcgcacaaa
     9781 ctggcgctgt aactggtttt gcagacgaat gcccagcacg gaaggcggca gcgttggcaa
     9841 aagcatcagt gaacaaagta gtttttcatt caaccaacgc cacagtttgt catcggccag
     9901 gccgaagcag gcaggcatca ggatcatttc gcaggtattg gcgacaggaa taagcgcatc
     9961 aagtaacagc ggccagtttt cttcattatc aaggaaacgg gcgatattca cagcgcgaaa
    10021 ttcggtggcg ttattgcgca ggacatccag ttccggcagc tctatttctg cggtttcaac
    10081 cgaaagatcc agttcgtgca acgaagccgc cgcaagatgc gcctgaaaat ccatcaggcc
    10141 gcttatcccg accacacata ttttcttcgc gggcaacggc cagacgggga cttctggcga
    10201 acttagccag gtagagcgca gagtgccgag cggcgtaatc cgctggtgag ccagttctac
    10261 gctgccctgc aattgcgcac cgctttcagc gattaatgcc tgcgcctggc aagcgagatc
    10321 gagcacgcgt tgtggcccga gaagagagta aggatgtgca ggtgcctgct gacgcaaaga
    10381 ttccagtcca ctgtgaatgt ctgccacggg ttgaccatct ggcagatggc tcagcaaatc
    10441 cagcgaaccg gatgagaaat gcaacgcgct ttgaccacgc gtgacaatgg cacagcgcag
    10501 gccgtgtttt tgcagttgca ggccacagag taatccggcg aggccgccgc ccataatgac
    10561 agtatcaaag cgcatctttc tgctccttct ccagaccaca cagtccctga taaacccagc
    10621 gggtaaattc gctttcgcgc agtgcgtctc cccaggcgat gggttgcacg cctttccagc
    10681 gttcgttaag gaaggtggaa agttgctcga tagattgcgc ggacgtcgtg acgttaaacc
    10741 gttgcagcag tccggcagcg cggcaggcgc agagttcgcc ctggcaggtg cccatcccca
    10801 cacgggtacg acggcgtaaa tccagcaggc tattaacgtt taaattttct accgcgtact
    10861 gcacttcacc cgcagttacc gcttcgcact cacataccag gctacggtgc agacggcctt
    10921 cgctcagcca ggcaggcgtg cgatcgccat gacgataaac cgcagaaccg cgcagcgggg
    10981 caggcaggga gatgacttta cgcaaggtaa cttcagcggg ttcttgtgaa ccaggcagtg
    11041 ccagatcggc agtcgtacag gggcgcgtgt tgcccagttt gcggcatacc gcgtcggtag
    11101 cccattcagc catcagccga taggtcatca gtttgccacc ggtgatggtg ataaatccgt
    11161 ccagaccatc gcgttcagca tggtcgagca gcacgatgcc acggctgacg ttacgtccgc
    11221 tcggatcgtc atcgctggca accagcgggc gcacgccaga ataggcccgc aaaatgcgcg
    11281 ttttcgccat cacgggggcc agtttttccc cttcacgcag cagaatatca acctcttctg
    11341 ccgtcactcg attatcgtca atctcgttgt aatcaatacg taaagaggtg gtaccaatca
    11401 gcgaaatggt atcgccaggc accagaatat cggcgtcgga aggtttacgg cagcggttga
    11461 tcacatgctg gttaatgcgg tgatccatga tcagcagcga tcctttcgcc gggaacatgc
    11521 gaatgcgcag atcggcatat tcggcaatgt gttgccccca gatcccagcg gcattaacca
    11581 cgacaggtgc atgaagggcc tgagtttcgc cggtgagatg gttacgtaca cgaacaccgc
    11641 acaccgtcgc gccttcacga atcagccccg tgacttcatg agcggtaagg ataacggcac
    11701 cgtgttcttt ggcatccagc atgtttgctg cggtcagacg aaatggatca acggtgccat
    11761 ccggaacttt caccgcgcca atcagtgccg ggttaacggc aggttcgata atgcgcgctt
    11821 gctgcgggtc tatagcttct gcgctgatcc ctgcttcttc gcaggcgcga ataaaagtgg
    11881 cctggaatga gaggtcatct tccggcaggg tgataaacag gccgttggtt ggttcaacgc
    11941 agtgacgtgc aatgcgtttc aggatctggt tttcactaat gcattcgcgg gccgattccg
    12001 catcggttac cgcatagcgc gcaccgctgt gcagcaggcc gtggttacgc ccggtggcac
    12061 cggttgcgat gtcgtggcgc tcaaccaaaa tcacgcgcag cccgcgcagg gcacagtcgc
    12121 gggcaatccc ggctcccgtt gcgccgccgc caatgataat cacgtcactt gattgcgagt
    12181 cgcgagtttt cattgttatc cctctgaagt tcgtttttta ccatttagcc atagtaaaaa
    12241 catgaattgt ttgatttcgc gcatgttcgc tcataattcg aaagtgaaac gtgatttcat
    12301 gcgtcatttt gaacattttg taaatcttat ttaataatgt gtgcggcaat tcacatttaa
    12361 tttatgaatg ttttcttaac atcgcggcaa ctcaagaaac ggcaggttct ctcactgaat
    12421 caggctgtta atcataaata agaccacggg ccacggaggc tatcaatgtt gagtattttt
    12481 aaaccagcgc cacacaaagc gcgcttacct gctgcggaga tcgatccgac ttatcgtcga
    12541 ttgcgctggc aaattttctt ggggatattc tttggctatg cggcttacta tttggttcgt
    12601 aagaactttg cgcttgctat gccttatctg gtcgagcagg gattctctcg cggtgattta
    12661 ggtttcgcgc tttcggggat ctcgatcgct tatggatttt cgaaattcat aatgggttcg
    12721 gtatcagatc gctcgaatcc gcgcgttttc ctccccgcag gtttgattct ggcggcggca
    12781 gtgatgttgt ttatgggctt tgtgccatgg gcgacgtcga gcattgcggt gatgtttgta
    12841 cttttgttcc tctgcggttg gttccagggg atggggtggc cgccgtgtgg tcgtactatg
    12901 gtgcactggt ggtcgcagaa agaacgtggc ggcattgtgt cagtgtggaa ctgtgcgcac
    12961 aacgttggtg gtggtattcc gccgctgctg ttcctgctgg ggatggcctg gttcaatgac
    13021 tggcatgcgg cgctctatat gcctgctttc tgcgccattc tggtggcatt attcgccttt
    13081 gcgatgatgc gcgatacccc gcaatcctgt ggcttgccgc cgatcgaaga gtacaaaaat
    13141 gattatccgg acgactataa cgaaaaagcg gaacaggagc tgacggcgaa gcaaatcttc
    13201 atgcagtacg tactgccgaa caaactgctg tggtatatcg ccatcgccaa cgtgttcgtt
    13261 tatctgctgc gttacggcat cctcgactgg tcaccgactt atctgaaaga ggttaagcat
    13321 ttcgcgctgg ataaatcctc ctgggcctac ttcctttatg aatatgcagg tattccgggc
    13381 actctgctgt gcggctggat gtcggataaa gtcttccgtg gcaaccgtgg ggcaaccggc
    13441 gttttcttta tgacactggt gaccatcgcg actatcgttt actggatgaa cccggcaggt
    13501 aacccaaccg tcgatatgat ttgtatgatt gttatcggct tcctgatcta cggtcctgtg
    13561 atgctgatcg gtctgcatgc gctggaactg gcaccgaaaa aagcggcagg tacggcagcg
    13621 ggctttaccg ggctgtttgg ttacctgggc ggttcggtgg cggcgagcgc gattgttggc
    13681 tacaccgtgg acttcttcgg ctgggatggc ggctttatgg taatgattgg cggcagcatt
    13741 ctggcggtta tcttgttgat tgttgtgatg attggcgaaa aacgtcgcca tgaacaatta
    13801 ctacaaaaac gcaacggagg ctaatggcat gaaattgacg ctgaaaaacc ttagcatggc
    13861 gatcatgatg agcactatag tcatgggaag cagtgcaatg gcggcggaca gcaacgaaaa
    13921 aatagtcatc gcccatcgcg gtgccagtgg atatttgccg gagcatacgc tgccagcaaa
    13981 agcgatggcg tatgcgcagg gagcggatta tctggaacag gatttggtga tgaccaaaga
    14041 cgaccatctg gttgttctgc atgaccatta tctcgatcgt gttactgatg ttgccgatcg
    14101 tttcccggat cgggcgcgca aagacggtcg ttactacgcg atagatttca cgctggatga
    14161 aattaagtcg ttgaaattta ccgaaggttt cgatattgaa aacggtaaaa aagtgcagac
    14221 ttatccgggg cgtttcccaa tgggtaagtc cgacttccgg gtgcacacct ttgaagaaga
    14281 gattgaattt gttcaggggt taaatcactc taccgggaaa aatatcggta tttatccaga
    14341 aatcaaagcg ccgtggttcc atcatcagga agggaaggat attgcggcaa aaacgctgga
    14401 agtgctgaag aaatatggtt acaccggtaa agacgataaa gtttatttgc aatgttttga
    14461 tgctgatgag ctgaagcgta ttaagaatga gctggaaccc aaaatgggca tggagctcaa
    14521 tctggtacag ctgattgcct ataccgactg gaatgaaacg cagcagaaac agccggatgg
    14581 aagctgggtt aattacaact acgactggat gtttaagccg ggtgccatga aacaggtggc
    14641 ggaatatgca gatggtattg gtccggatta ccatatgttg attgaggaga catcgcagcc
    14701 gggtaatatc aaactcactg gcatggtgca agatgctcag cagaataaac tggtagtgca
    14761 tccttatacc gtgcggtcag ataaactgcc tgaatacact cctgatgtga atcagttata
    14821 tgatgctctg tataacaaag cgggtgtaaa tgggctgttt actgatttcc ctgataaggc
    14881 agtaaaattt cttaataaag agtaatgtta ctgcaggatg gcgttcatgc gccatcctgt
    14941 ctcatatcaa atttttccaa tcgccactaa ttgttccatg cacatttgta gtaaaaacag
    15001 tggactgcct tctaccggct tttctttagc cagcaacgaa atctgtcgat agaaagttgg
    15061 ttcgagagga acggaacaca attcacctgc aatgcttttc agcgttaact ctggttgtaa
    15121 ggcgatgcct aatccctgtc gaataaaatt catcgctgta tccgcctgat taaattcaca
    15181 ttttatctgc tgggcgcact attagtggta gtggctggtg gaatctatgc gctgcaaatg
    15241 aagggtaaaa gcggtgtcaa gctagtagtg gcaaaagaaa ttcattaata cgacacgttt
    15301 cattaagatt ttcctcaggt aaccacctat agtcattgtc gttaataaaa acgacaggcg
    15361 atagggtatg gcagtttcag caaagtacga cgaatttaat cactggtggg caacagaggg
    15421 cgactgggtt gaagagccta actatcgccg taatggtatg agcggggtgc aatgcgtcga
    15481 gcgcaacggc aaaaagctgt atgtaaagcg catgacgcat catctgtttc attctgtacg
    15541 ttatccgttc ggccgaccaa cgattgtccg tgaggttgcg gtaattaaat aactggaacg
    15601 ggccggtgtc atcgtgccga agatcgtttt tggtgaagcg gtgaaaattg agggtgaatg
    15661 gcgagcgttg ctggtgactg aagatatggc ggggttcatc agcattgctg actggtatgc
    15721 ccagcatgca gtatcgcctt attctgacga agtacggcaa gccatgttga aagcagtggc
    15781 gctggcgttt aagaaaatgc atagcattaa tcgtcagcat ggctgttgtt atgttcgcca
    15841 tatctatgtg aaaacagaag gtaaggcaga agctggtttt cttgacctgg agaaaagtcg
    15901 tcgccgcttg cgtcgcgata aagcgattaa tcatgacttc cgccagctgg aaaaatatct
    15961 ggagccaatc cccaaagcgg actgggagca ggtgaaagcg tattactacg ccatgtaatc
    16021 gttgtaggcc agataagaca cataagcgtt gtatctggca ctacaaccaa ttcacatctc
    16081 gatttcaata tcgccttttg cccggcaaca acagggcaaa atttcccccg gctgaataaa
    16141 ggctaacggt tcggcaatcc agtcaacttg acctgcaacc aggcgtgtgc gacaggagcc
    16201 gcagtaacct tcgcgacact ggtactcaac cgccacattg tgggattcca gcgctgccag
    16261 aagggaaggg tgttcatcct ggcacagcag ttgtgtgcca gtgatgcgca gggtaacgcg
    16321 ggccatcaga gctggaagtt actcaaatcg tcggtgtcca cttccgagtc aatctgcccg
    16381 accagataag aactgacttc cacttcctgc ggagcaacct gcacgttatc agacaccagc
    16441 caagtgttga tccacgggat cgggttggag cgcgtctgga acggcagatc caaaccgact
    16501 gcctgcatac ggatattggt gatgtattca acgtactggc agagaatgtc tttattcaga
    16561 ccaatcatcg aaccgtcgcg gaacagataa tccgcccagt ctttctcctg ttgagctgcc
    16621 tgaacaaaca ggtcatagca ctcctgctta cactcttcgg caatttccgc catctcagga
    16681 tcgtccgcgc cgctgcgcag cagattcagc atatgctggg tgccggtcag gtgcagggct
    16741 tcgtcgcggg caatcaggcg aataattttg gcgttgcctt ccatcaattc gcgttctgca
    16801 aaggcaaagg aacaagcaaa gctgacgtag aagcggatcg cttccagcgc gttaacgctc
    16861 atcaggcaaa gatacagttt tttcttcagc tcgcgcaggc taacggttac ggttttaccg
    16921 ttaacggtgt gggtgccttc gcccagaagg tgccagtagc tggtcatttc gatcagttca
    16981 tcgtaatagc tggagatccc ttccgcacgt ttctggatct gctcgttggt gacgatatcg
    17041 tcaaacacaa cagacggatc gttaacgata ttacgaatga tatgagtata ggaacgggaa
    17101 tgaatcgttt ctgagaacgc ccaggtttcg acccaggttt ccagttccgg aatagaaata
    17161 agcggcaata gcgccacgtt cggactacga ccctgaatgg aatccagcag cgtctgatat
    17221 ttcaggttgc tgataaaaat gtgtttttcg tgctccggca gcgcctggta atcgatacgg
    17281 tcgcgggaga cgtcaacttc ttccggacgc cagaagaaag agagctgctt ttcgatcagc
    17341 ttttcgaaga tgtcatattt ttgctgatcg tagcgggcca cgttgaccgg ctgaccaaag
    17401 aacatcggtt ctttgagctg atcatttttc gtctgtgaaa aggtggtata tgccatgagt
    17461 gtgtcctgtt gggagtttaa tgccggataa ggcattttac gccgcatcct gtgcctgatg
    17521 cgacgctggc gcgtcttatc aggcctacaa atccgagccg taggccggat aaggcgttca
    17581 cgctgcatcc ggcatctcaa tatcagatct tacatgcgcc gctttcgcag ccatcgtcct
    17641 ggattgacgg taccagatcg tcctgcgcgt cttcagcgcc gtcacgggtg ttctgataat
    17701 acagcgtttt gaccccgaat ttgtaggcgg tgagcaggtc tttcagcaac tgctgcatcg
    17761 gcacttttcc tgacgggaaa cgtgacggat cgtagttggt gttagcagag atcgactgat
    17821 cgataaattt ctgcatgata cctaccagtt gcaggtagcc atcgttaccc ggcatttccc
    17881 acagcagctc ataggcgtcg tgcaggtgct cgtagtccgg caccacctgg cgcaaaatac
    17941 cgtctttcga cgctttgatg ctgacgtaac cgcgcggcgg ttcaataccg ttagtggcgt
    18001 tagagatctg cgaagaggtc tcggacggca tcagggcaga aagcgtggag ttacgcaggc
    18061 cgtgcgtttt gattgattca cgcaatgctt cccagtcgta atgcagcggc tcattagcga
    18121 tggcatccag atctttctta taggtatcga tcggcaggat ccctttcgcg taagtggttt
    18181 cgttaaacca cgggcactcg ccttgctctt tagccagctc attagacgct ttcagcaggt
    18241 aatactgaat cgcttcgaag gttttatgcg tcaggttgtt ggcgctgccg tcggagtagc
    18301 gtttaccgtg cttcgccagg tagtaagcga agttgatcac accaataccc agcgtacgac
    18361 gacccatcgc tccacgtttg gcggccggga tcgggtaatc ctgataatcc agcagcgcgt
    18421 caagtgcacg aaccgccaga attgccagct cttccagttc atccaggtta ttaattgcgc
    18481 ctaggttgaa agcagacagc gtacacagcg cgatttcacc gttctcgtcg ttgacgtcgt
    18541 tcagcggttt ggtcggcagg gctatctcca ggcacaggtt agactgacgc actggcgcga
    18601 tggccggatc aaacgggcta tgggtattac agtggtcaac gttctgaata tagatacgac
    18661 cggtagacgc acgttcctgc atcatcagcg agaacagctc aacggctttc acacgctgct
    18721 tgcggatgct gtcgtctttc tcatatttgg tatacagacg ttcaaactct tcctgatcgg
    18781 cgaagaacgc gtcgtacagc cccggtacgt cggacgggct gaacagggtg atatcttcac
    18841 ctttcagcag acgggtatac atcagtttgt tgatttgtac cccgtagtcc atatgacgca
    18901 cgcggttgcc ttccacacca cggttgtttt tcaacaccag caggctttcc acttccagat
    18961 gccacatcgg gtagaacagc gttgccgcac cgccgcgcac accgccctga gagcaggatt
    19021 tcaccgctgt ctggaaatgt ttgtagaacg gaatgcagcc ggtatggaac gcttcaccac
    19081 cgcgaatcgg gctacccagc gcacgaatac gcccggcgtt gatgccgatc ccggcacgct
    19141 gggaaacgta tttaacaatc gcgctggagg tggcgttgat ggaatccagg ctgtcaccgc
    19201 actcgatcag tacgcaggag ctgaactgac gagtcggggt acgcacgccg gacatgattg
    19261 gcgtcggcag cgaaatttta aatgtggaaa ccgcgtcgta aaaacgcttc acatattgca
    19321 ggcgcgtttc acgcgggtag ttcgagaaca ggcacgcggc aaccagaata taaaggaact
    19381 gggcgctctc atagatttcg ccggtcacgc ggttctgtac cagatatttg ccttccagct
    19441 gcttaacggc agcataagag aaggtcatat cacggtcgtg atcgataaac gtgtccatct
    19501 gcttgaactc ttcttccgtg tagtcttcca gcagatgatt atcgtatttg cccatctcga
    19561 ccattttcac cacgtggtcg tacagcgcag gcggctcaaa ctggccgtag gcttttttac
    19621 gcaggtggaa gatcgccagg cgcgcggcga gatactgata atccggcgca tcacgggaga
    19681 tcaggtctgc ggcagccttg ataatggttt cgtggatgtc agaggtcttg ataccgtcat
    19741 aaaactgaat gtgggagcgc agctcgacct gggaaatcga aacgttatgc agtccttctg
    19801 ccgcccaatc cagaacgcga tggattttgt cgagattgat gcgctctgtg ctaccgtcgc
    19861 gctttgtcac cagcagattc tgattcatgt atgtcgtacc tgtttttgga attctttccg
    19921 caatagtttc atgcagacta cctgtagtac gggaagctgc ttagaacact atatataggg
    19981 ggtatgtttg ggattcactg caagatagtg tgaaaatggc cctcttgcaa gtgcataact
    20041 ttgtggataa ctcaggaagg aaaaagtggc tttcgcgcac cttaggtcag acaaggtgtc
    20101 cgggaaagtc aatgggaaga gaaaaatttg ttaaaaataa ctgttcggcg atttcttgaa
    20161 cggcagaata ggttggataa attgatatag atggtttatt aatctttttg ctaatgattg
    20221 caaaaattga tctaagcacg agtcatcaca taatctgatg tatcagttgt ggtcaatata
    20281 taagcgaatg taagcgattg ttgatgtcga aatggttgtt ttctattcgg ttattgttaa
    20341 atgcgaagcc attttgttaa tttaccttat attataaaaa aattattcat tactttaatt
    20401 tagtttaaat ataactggta tcccttaagg ttattaactt gaattatgac gcactgatat
    20461 tattcatcaa ataataacaa aatagccatt gcatcgggtt ggacaattta caaaagaaag
    20521 ataacccata ttgtataatg cgtttaaata tgccgtctta tataaaatga tggtcagatt
    20581 aatatgcgga ttatctttct
//
LOCUS       DAJVUJ010000064        20463 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.064, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000064 DAJVUJ010000000
VERSION     DAJVUJ010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20463
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.064"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..878
                     /locus_tag="OQQ48_002123"
     CDS             <1..878
                     /locus_tag="OQQ48_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CS1-pili formation C-terminal domain-containing
                     protein"
                     /protein_id="HCS1952893.1"
                     /translation="DQTSWQLSHTFYNDSFSHKELGVTVGGLNTDTINSAVNGRWDGQ
                     YGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGASGTDDLLGAVLVDVKG
                     FSEQDEESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGFVEVNDSSQGNQQGTTN
                     IINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKKFPIVGLNSAMLLVAED
                     GGFTLEINGSEKELYLLSGQQFLKCPLSVEKKRASIRYSGDVTCSVVTYSQLPELKQP
                     KLRGNVQTAQREVAP"
     gene            875..1964
                     /locus_tag="OQQ48_002124"
                     /pseudo
     CDS             875..1964
                     /locus_tag="OQQ48_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
     gene            complement(2052..3350)
                     /gene="exuT"
                     /locus_tag="OQQ48_002125"
     CDS             complement(2052..3350)
                     /gene="exuT"
                     /locus_tag="OQQ48_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HCS1952894.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSRGGLA
                     IARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFSLVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQESHNDPAPQH"
     gene            3833..5245
                     /gene="uxaC"
                     /locus_tag="OQQ48_002126"
     CDS             3833..5245
                     /gene="uxaC"
                     /locus_tag="OQQ48_002126"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCS1952895.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            5260..6747
                     /gene="uxaA"
                     /locus_tag="OQQ48_002127"
                     /pseudo
     CDS             5260..6747
                     /gene="uxaA"
                     /locus_tag="OQQ48_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
     gene            6830..7381
                     /locus_tag="OQQ48_002128"
     CDS             6830..7381
                     /locus_tag="OQQ48_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HCS1952896.1"
                     /translation="MTAYWLAQGVGFIAFLIGITTFFNRDDRRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTRALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAVDERG"
     gene            complement(7386..8630)
                     /gene="sstT"
                     /locus_tag="OQQ48_002129"
     CDS             complement(7386..8630)
                     /gene="sstT"
                     /locus_tag="OQQ48_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HCS1952897.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDLSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(9246..10211)
                     /gene="alx"
                     /locus_tag="OQQ48_002130"
     CDS             complement(9246..10211)
                     /gene="alx"
                     /locus_tag="OQQ48_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HCS1952898.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINARVNYRHDKQRGE"
     gene            complement(10495..11481)
                     /locus_tag="OQQ48_002131"
     CDS             complement(10495..11481)
                     /locus_tag="OQQ48_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCS1952899.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTASLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLLHSKVSDSVLESEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            complement(11560..12243)
                     /locus_tag="OQQ48_002132"
     CDS             complement(11560..12243)
                     /locus_tag="OQQ48_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HCS1952900.1"
                     /translation="MQPRLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNASKQL
                     TWSDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKNYDE
                     ASGMQKALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRAIWL
                     AKQYSIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHSQPKYLGPSVMIGPF
                     SEHGCPAKE"
     gene            complement(12320..12823)
                     /locus_tag="OQQ48_002133"
     CDS             complement(12320..12823)
                     /locus_tag="OQQ48_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HCS1952901.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            12908..14044
                     /gene="rlmG"
                     /locus_tag="OQQ48_002134"
     CDS             12908..14044
                     /gene="rlmG"
                     /locus_tag="OQQ48_002134"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HCS1952902.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            14329..14643
                     /gene="higB"
                     /locus_tag="OQQ48_002135"
     CDS             14329..14643
                     /gene="higB"
                     /locus_tag="OQQ48_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HCS1952903.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNMIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            14640..15056
                     /gene="higA"
                     /locus_tag="OQQ48_002136"
     CDS             14640..15056
                     /gene="higA"
                     /locus_tag="OQQ48_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HCS1952904.1"
                     /translation="MIAIADILQAGEKLTAVVPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLADLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(15101..17119)
                     /gene="fadH"
                     /locus_tag="OQQ48_002137"
     CDS             complement(15101..17119)
                     /gene="fadH"
                     /locus_tag="OQQ48_002137"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HCS1952905.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLHTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEDGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSESGGQFNIAKQIPGKEEFYETLRYYHRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELNARRAIAQGTRLALEI"
     gene            complement(17345..19697)
                     /gene="ygjK"
                     /locus_tag="OQQ48_002138"
                     /pseudo
     CDS             complement(17345..19697)
                     /gene="ygjK"
                     /locus_tag="OQQ48_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
     gene            complement(19714..>20463)
                     /gene="ygjJ"
                     /locus_tag="OQQ48_002139"
     CDS             complement(19714..>20463)
                     /gene="ygjJ"
                     /locus_tag="OQQ48_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HCS1952906.1"
                     /translation="VPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWW
                     GDFTPDLGYVIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYD
                     DKYTYPAMMNGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRAD
                     GLDVPGSESGMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGG
                     RFATAFDSRFYMKNASHDPSDQIFLMQYFYW"
BASE COUNT         5003 a         5357 c         5363 g         4740 t
ORIGIN      
        1 gcgatcaaac gtcatggcag ttatcgcata ccttttataa tgattcattc agccataaag
       61 aacttggcgt aaccgtcgga ggcctgaaca ccgataccat aaacagcgcg gttaacgggc
      121 gttgggatgg gcaatacgga aatgtctacg ctaccgtatc tgatagttat gaccgtaaga
      181 atcatgatca tctttcggcc tttacgggca cttatagctc tacgctggct gtcagtcgct
      241 atggcgttaa tttgggagcc agtggtacag acgatttgct gggtgcggta ttggtggatg
      301 tgaaaggctt ctctgaacag gatgaagaga gtcaggatct gcaactcgaa gcgcgggtgg
      361 caggcagccg aactttgcag cttggtcaaa gcgacagtgt gttgttccct tatcctggat
      421 ttcagtctgg ttttgttgag gttaacgaca gtagccaggg caatcagcag gggacaacaa
      481 acatcattaa cggtgcaggg aatcgtgaat taatgttgtt gcctggcaag ctgcgctatc
      541 gcgaagtgtc tgccagcttt aactacaact atatcggtcg tttattactg ccggcagcgg
      601 tgaaaaaatt cccgattgtg gggctgaaca gcgccatgtt actggtagct gaagatggcg
      661 gatttacact tgaaattaac ggcagcgaaa aagagctcta tctgctttcc gggcagcaat
      721 tccttaagtg tccactgagt gttgaaaaga aacgcgccag cattcgttac agcggagatg
      781 ttacttgtag tgtggtgact tattcacaat taccggagtt gaaacagcct aaattacgtg
      841 gaaacgttca gacggcgcaa agggaggttg caccatgaga aatcgattga ttgcggcgat
      901 attgggcttg tttggcacgc tcactggcgt tcaggcagct cctgacgtga ccagtgaaat
      961 tacgtatgat ttggcatctg gtagagcgga ttattacttc tggaaggatg aagcgtcggc
     1021 aggaaataat ggatatatgt ggtatgaatg ttcgtatcct gacctccaac aaacctgtac
     1081 agctaatgga aatatatcga cagtacaaat ctatttaact gaacaacgca gtgggatgcg
     1141 ttggccggta aaactcaaag gatttaaaac agccattgta agtagcgatg aagcgccgct
     1201 aggatgcaag ggggggcaaa gggcttcaga cgaatcttaa ggattctaat agatcttcat
     1261 gtacagaaga tggtcaacat tattatatat acgatacaaa gtttcttacg ctctaccttg
     1321 agcagacaga gatgaagaat ttgccgattg gtggcgtctg gaaggggaaa gttaaattac
     1381 attcgaacag cccggcccag gactatttcg caaatattac cctgaatacg ctcgacccca
     1441 accatattga cgtgttcttc ccggagttcg cccacgccac gccgagggtg cagttagact
     1501 tgcatccaac aggaagcgtt aacggcagca actacgcgca agatctgacc atgttggaca
     1561 tgtgcctgta cgatggtttt aacggtaatg ccatcagtta tgaaatcatg ctcaaagatg
     1621 aagggcgacc agctgcaggg cgcagagacg gtgacttctc tatctatcgt cagggaggaa
     1681 cctctaccga cgagggagaa cgcattgatt accgggtcaa aatgtacaac ccggaaaccg
     1741 gtgggcaaat tgatgtgcgc aataatgaaa atatggtctg gaacagcatt aacctgaaaa
     1801 gtgtgcgtcc ggtggtactg cccggtattc gctatgccgt gatgtgtgtg ccgacaccct
     1861 tggctctggc agtcgataaa ttcagcgtga tggataaaca ggccgggtac tacatgggca
     1921 aattgtcagt aatctttacg ccttccttgc caaccatcaa ttaaccctgc aaagcggaca
     1981 aacgcttttt tctatataaa cgacgaagcc gcctgataag gcggcttcgt cgttataact
     2041 tacgttaaac gttaatgttg cggtgcggga tcgttatgtg attcctgcgc tacctcaatc
     2101 gccggtttgt tctgcaacac ggtccagata accagcgcac ctaacaggtc gaacactgcc
     2161 agtactgcga acagcgggct gaagccgatg gtgtcagcca gtgcaccgac aaccagcgaa
     2221 aacagcgtac ttgccagcca tgcggacatc ccggttaaac cgttcgccgt cgccacttcg
     2281 ttacgaccga acacgtcaga agagagcgta atcagcgcgc cagacagtgc ctggtgagca
     2341 aaaccaccga tacacagcag cataattgcg acatacgggt tggtgaacag gccgatcata
     2401 cccgggccaa tcatcagcac tgcacccagc gttacgacca tcttacggga aacgatcagg
     2461 ttcacaccaa accaacgctg gaacagcggc ggcaggtaac caccgaggat acaaccgagg
     2521 tcagcaaaca gcatcggcat ccaggcgaac atcgcgatct ctttcaggtt aaagccgtaa
     2581 actttaaaca tgaacagcgg gatccacgcg ttaaaagtac cccaggctgg ttctgccagg
     2641 aaacgcggca gcgcgatacc ccagaactga cggttacgca ggatctgacc aacggacatt
     2701 ttcttcgccg tgctcacctg gtgctgggct tcctgaccat taataatata gtcgcgttct
     2761 tcatcggtca gatgcttctg gtcgcgcgga tgtttataga aaatcagcca tgccatcgcc
     2821 cagataaagc tcaatgcacc ggagatgata aatgccatct gccagctgtg cattacgatt
     2881 gcccatacca ccagcggcgg cgcaatcatc gcaccaatcg aagaacctac gttaaagtaa
     2941 cctaccgcga tggaacgctc tttcgccggg aaccattcgg agctggcttt cagacccgcc
     3001 gggatcatcg ctgcttccgc ggcaccgact gcaccacgag caatcgccag gccacccctg
     3061 ctaccagcca gcgcggttgc accacagaat acggcccaca gtacagcaaa cattgcataa
     3121 ccgattttcg tacccagcac atccagtaca tagcccgcta ccggttgcat gaccgtataa
     3181 gcagcagaat aggctgcgat gatataggaa tactgttggg tggagatgtt taactcttcc
     3241 atcagagttg gcgcagctgc cgccacagtg ttacgcgtca ggtaaccaag cacggtgcca
     3301 agcgtcacca gtgcgatcat ataccaacgt aaccctttaa ttttacgcat gtaaaacctc
     3361 atcgttttgt tatatccgca ggaagcgcgg ccatcatccc gccagaggct gaagatgtta
     3421 gttacgaccg ggtaaccgaa aaaaaaccgg cacgccccga acgttgccat gaaagtttcg
     3481 tgctaattcg gcttccgtac cggttaaccc gattcatcta acaaatgaat gaatcggcaa
     3541 tttgcattcc gtcgacttat gatttgcgac ggcagaaaga tgacttgtca tacaacttta
     3601 aaaggtgaga gccatcataa atgtgcgaat atttgtaggg acattacctg acgacagcga
     3661 ggccagtact ggcgcggcct gcagcgagat ttaccacttt gagagtaatt tttttaacta
     3721 cgtttattga tctaactcac gaaaatatct tcggactctg gaaattggtg tgataacttt
     3781 gtcagcatcg caccataagc aagctagctc actcgttgag aggaagacga aaatgactcc
     3841 gtttatgact gaagatttcc tgttagatac cgaatttgcc cgccgtctgt atcacgacta
     3901 cgcaaaagac cagccgattt tcgattacca ttgccatttg ccgccgcagc agattgcgga
     3961 agactatcgt tttaaaaacc tgtatgacat ctggctgaaa ggcgatcact acaaatggcg
     4021 cgctatgcgt accaacggtg tggccgagcg tctgtgtacc ggtgatgcgt ctgaccgtga
     4081 aaaatttgac gcctgggcgg cgactgttcc gcacactatc ggcaacccgt tataccactg
     4141 gacgcacctc gaactgcgtc gtccgtttgg tatcactggc aaattgcttt ctccgtcaac
     4201 tgccgatgaa atctggaacg aatgtaacga actgctggcg caggataact tctccgcgcg
     4261 cggcatcatg cagcagatga acgtgaaaat ggtcggcacc accgatgacc cgatcgattc
     4321 tctggagcat cacgcagaga tcgccaaaga cggctctttc accatcaaag tgctgccgag
     4381 ctggcgtccg gacaaagcct tcaacatcga acaggcgacc tttaacgact acatggcgaa
     4441 gctgggcgaa gtttccgata ccgacattcg ccgctttgct gacctgcaaa ctgccttgac
     4501 caaacgtctg gatcacttcg ccgctcacgg ctgtaaagtg tctgaccatg cgctggacgt
     4561 ggtgatgttt gctgaagcga acgaagcgga actggacagc attctggcgc gccgtctggc
     4621 tggcgaaacc ctgagcgagc acgaagtggc acagttcaaa actgcggtac tggtgttcct
     4681 tggtgccgaa tatgcacgtc gcggctgggt acagcagtac cacattggcg cgctgcgtaa
     4741 taacaacctg cgtcagttca aactgctggg gccggatgta ggctttgact ccatcaacga
     4801 ccgtccgatg gcagaagagt tgtctaagct gctgagcaag cagaacgaag aaaacctgct
     4861 gccgaaaacc attctgtact gcctgaaccc gcgcgataac gaagtgttgg gcaccatgat
     4921 cggtaacttc cagggcgaag gtatgccggg caaaatgcag ttcggttccg gctggtggtt
     4981 taacgatcag aaagacggta tggaacgtca gatgacccaa ctggcgcagc tcggtttgct
     5041 gagccgcttt gtcggtatgc tgactgacag ccgtagcttc ctgtcataca cccgtcacga
     5101 atacttccgc cgcattctgt gccagatgat cggtcgctgg gtggaagcgg gcgaagcacc
     5161 ggcggacatc aacctgctgg gcgagatggt gaaaaatatt tgctttaaca atgcgcgtga
     5221 ctacttcgcc attgaactga actaaggtct ggggttgata tgcaatacat caagatccat
     5281 gcgctggata acgtcgcggt cgctttagcg gatttggctg aaggcacaga agtcagtgtc
     5341 gataaccaga ctgttacgct gcgccaggat gttgctcgtg gacataaatt tgcgttaacg
     5401 gatatcgcaa aaggggccaa tgtcatcaaa tatggcctgc cgattggtta tgcattggcg
     5461 gatattgcgg cgggggaaca cgtccacgcc cacaatacgc gcacgaatct gagcgatctg
     5521 gatcagtatc gctatcaacc tgattttcag gatctgcctg cgcaagcggc agatcgtgaa
     5581 gtgcagatct atcgtcgcgc taacggcgat gtcggggtgc gtaacgagct gtggatcctg
     5641 ccaaccgtgg gctgtgtcaa cggcatcgcg cggcagatcc agaaccgttt tctgaaagag
     5701 accaacaacg ctgaaggtac cgacggcgtg ttcctcttca gccacaccta cggctgctca
     5761 cagctgggcg acgatcacat taatacccgc accatgctgc aaaacatggt gcgccacccg
     5821 aacgcaggcg cagtgctggt gattggtctg ggctgtgaaa acaactaggt tgccgcattc
     5881 cgtgaaacgc tgggcgatat cgatcctgaa cgcgttcatt tcatgatctg ccaacagcag
     5941 gatgatgaga tcgaagccgg gatcgagcat ttgcatcagc tgtataacgt gatgcgcaac
     6001 gataaacgcg agccaggcaa actcagcgaa ctgaagtttg gtctggagtg cggtggttct
     6061 gacggtcttt ctggtattac tgctaacccg atgctggggc gtttctctga ctacgtgatt
     6121 gctaacggcg gtactaccgt actgaccgaa gtgccggaga tgtttggcgc agagcagttg
     6181 ctgatggacc attgccgcga cgaagcaacg tttgaaaaac tggtcaccat ggtcaacgac
     6241 tttaaacagt actttattgc ccatgatcag ccgatctacg aaaacccatc accggggaac
     6301 aaagcgggcg gtatcaccac gctggaagac aaatcacttg gctgtaccca gaaagcgggt
     6361 tccagtgtcg tggttgacgt gctgcgttac ggcgagcgtc tgaaaacgcc agggctgaac
     6421 ttgttaagtg cgccgggtaa cgatgccgta gcgaccagcg ccctggcggg tgcgggctgc
     6481 catatggtgc tgttcagtac tggtcgcggc acgccatacg gtggatttgt gccgacggtg
     6541 aaaatcgcca ccaacagtga actggcggcg aagaaaaaac actggctcga ctttgacgcg
     6601 ggtcagctga tccacggtaa agcgatgccg cagttgctgg aagaatttat cgacatcatc
     6661 gttgagtttg ccaacggtaa acaaacctgc aacgagcgta acgacttccg cgaactggcg
     6721 atcttcaaaa gcggcgtaac gctataaagt cgaaatgagt tgttaataaa aaacggcgtt
     6781 tcataatgtg aagcgccgtt tgcttttcca tacctgataa ggatttatca tgaccgcgta
     6841 ttggctggcc cagggcgtgg gtttcatcgc ctttctgatt ggtatcacaa catttttcaa
     6901 tcgtgacgac cgtcgcttca aaaagcagct ttcggtctat agcgccgtta ttggcgtaca
     6961 tttttttctt ctgggcacct atcccgctgg tgccagtgcc atccttaatg ccattcgtac
     7021 attgattacc ttacgcacgc gcagcttatg ggtaatggcg atttttattg tgctgactgg
     7081 cggaattggc ctcgcgaagt tccatcatcc tgtcgaacta ttgccggtta tcggtacgat
     7141 tgtcagtacc agggcgctgt tccgctgtaa agggctcacc atgcgctgcg tgatgtggtt
     7201 ttcaacgtgt tgctgggtga ttcacaactt ctgggcgggg tcgataggcg gcacaatgat
     7261 tgagggtagt tttctgctta tgaatggctt gaatatcatt cgtttctggc ggatgcagaa
     7321 aaggggaatt gatccgttta aagtagagaa aacccctccc gccgtagacg aaaggggtta
     7381 aacaattaat tacgcagggc gctatttgcc agacgatcgt cttccgcctg gcaagctgcc
     7441 gcagtgaaca gcacgtcagt tgaagagttc agcgcggttt cgcaagagtc ctgcaatacg
     7501 ccgatgataa agccgacggc aaccacctgc atggcgatat cgttcgaaat accgaacata
     7561 ttacaggcca gcgggatcag cagcagagaa ccccccgcca cgccggatgc gccacaggca
     7621 cacagagaag ccaccacgct caacagcagc gccgtgggca gatcgaccgg gatacccagc
     7681 gtattaaccg cagccagcgt caacacggta atggtgattg ctgcgcccgc catattgatg
     7741 gtggcaccca gcggaataga aacggaatag gtatcgcgat ccagattcag cttttcacac
     7801 agtgccatat tcaccggaat gttcgccgca gagctgcggg tgaagaaggc atacacgccg
     7861 ctttcgcgca ggcacagcag caccagcggg aacgggttac gacgaatttt ccaccacacc
     7921 agcaatgggt taaccaccag cgccaccagt aacatacagc caaccagcac gaccagcagt
     7981 tgcgcgtagc cccacagtgt ggagaaaccg gtggttgcca gggtagaaga aaccagacca
     8041 aaaataccaa tcggtgcgaa gcgaatgacc agtttcacca taaaggtgac ggcattcgac
     8101 agatcgttga ccaggttttt ggtggtctcg ttaccgtgac gcagtgcgaa accgaggcca
     8161 attgcccaca ccaggatccc gatgtagtta cctttcagca gcgcgtcgat ggggttggaa
     8221 accatgctca ttaccagccc gcgcatcact tctacaatgc ctgacggtgg cgaaatatca
     8281 cccgcgctac tggataagtg cagggtagaa gggaaggcga agctgaaaac gactgcggcc
     8341 agagcagcag agaaggtacc cagcagatag aggaacaaaa tagggcggat attggttttc
     8401 tgcccgtgct ggtggttagc aatagatgcc atcaccagca tcaacaccag gatgggggca
     8461 acggctttca gtgcgccgac gaacaaagta cctaacagac caacagcttc cgccgcgggt
     8521 tttgagatcc atgccagaag aatccccaga acaaggccga ccaggatttg ttttaccagg
     8581 ctgccatgag ccagacgccg gaatagcccc ggtgaacgtt gcgtagtcat ttttcgatcc
     8641 tttcattgtg ttgtcgcaca tccctggtgc gttctgtcga cgcatctttc aggatgtaac
     8701 aaagtgtttg caaggtcgag tataaggaaa gttacagctt cgggaagcga aaaatgctgg
     8761 attttaaaga gaggtcatat tttgcaactt aattgttaca tttgtttgac ataaacagag
     8821 ggaaggactg gctactaagg ctgattagat aacatatggg caaaactatt tggaatatga
     8881 gaacggttgc gctcctttaa tgtgctatgc catataaagt cactggcgca gggagcgcgc
     8941 agggggcagc caatcgccgc cgccccctgc acccccaggc tctggcgaac aaaatcgccg
     9001 ctgcgcgatt ccctcagctt atcccttgcg gctaccgggt cgggcgcaag ggaacgtccc
     9061 tgtaagatgc gccctcgccg cgcatccatg cgcgtcgccc cggcctccag ggaacgcttc
     9121 ggcgattttg acgccaccaa cacccgtgcg gtctttttgt aaagagctac aagccaatta
     9181 cataattaat agctcacctt tgtctggaat gagttatctc taggaatggc ttaatataca
     9241 gcgaatcatt ctccccgctg cttatcatgc cgataattca ccctggcgtt gataataaac
     9301 gtcatcacca gaatgccaaa caccacgccc agcgagacgg cgattggaat atggtagaag
     9361 tcgacaatca gcatcttgat accgataaac accaggatca ccgccaggcc atatttgagc
     9421 atcgagaaac gctctgctac gcccgccagc aggaaataca tcgcacgcag gccgaggatc
     9481 gcaaacaggt ttgaggtcag cacaatgaac gggtcagtgg tcacggcgaa gatagccgga
     9541 atgctatcca cggcgaaaat cacgtcgctc aattccacca gaatcagtac cagcatcagc
     9601 ggtgtggcgt acagcaatcc attcttacgc acaaagaaat gctcgttgtc gatggtgtcg
     9661 gtcatgcgca aatgaccgcg tagccagcgc accagcggct tgtcgccaat gcctgattca
     9721 tcttcatggg cgagcgccat cttcacgccg gtaaacagca ggaaggcacc gaagatatac
     9781 aggatccagt cgaactgcga aattagccag ctgccagtga agatcatgat ggtacgcaga
     9841 acaatcgccc cgagcacgcc atacaccagc acgcggcgtt gtaatgccgc cggaacagag
     9901 aaatagctga acaacatcag ccagacaaag acgttatcga ccgccagcga tttctcaatc
     9961 agataacccg tgagaaaggc cagtgcctgt ggatcggcaa cggcgcgacc ttcggtttgc
    10021 accagatacc accagaaagc ggcgttaaac agtaacgaca gcgtcaccca aaccagcgac
    10081 caggccgcag cctgtttcat ggtcatggca tgtgccccac gacgcccctg caacaacagg
    10141 tcgatagcca gcataatggc gacaacaaca gcgaatccgc cccatagcaa cggcgtgccg
    10201 acagtattca tagaagttcc ttacacataa aaaaaacggc caacgtcgga agacgtcagc
    10261 cgctgctttt tatgcataga cctcgccttc cggcaaggtc tcacttacaa caaaaaagga
    10321 atacgtctgc gtattccttt cgggttgccc ggtgaccgga tgtggttttt cacacatcgt
    10381 aatgacgatc gaccggcaat gaagttactc ccctttgcag gtaacaaagt atgacagacc
    10441 attcagccag tcaatggtct gttacatacc ttttacactg ttttactttg gcaattatag
    10501 ttttacgcta tctgccggaa aaatcacccc agtctggcgg cggatctcag tcagcagttt
    10561 ggcggtgatg cgactgaccg ccagccctgg atgatccacc agatgctcat ccaccagggt
    10621 agcaaacagc tctgcttcgt agagcatggt attaatatgc tgcggctggg tgagatcctg
    10681 catctggctg ccacgcggca cgaagcacac tttctggcat tcagacagtt tttcaatcac
    10741 cagcgatccc gcttcgccct gaatctcgct ttccaggaca gaatcactga cctttgagtg
    10801 cagcaacgta acgctgaaat caccgtaatc catcaccacc acgccgtggg catctacgcc
    10861 gcttgccaac aaactggcgg ttgcctgcac gcttttcggt tcaccgaata atgccaccgc
    10921 cgacgccagg cagtaaaagc cgatatccat aattgaaccg ttagagaatg ccggattaaa
    10981 ggtgttggga ttctcaccat ccaggtaacg ttgataccgc gaggaatact ggcaatagtt
    11041 gaaaaagact ttacgcaatt tgccgacttt cggcagcgcc tggcgcagca aatgaaagtt
    11101 cggcaggctg gcggttttaa atgcctcaaa cagcaccacc tgattttccc gcgcacaggc
    11161 aatggcggca tccacttccg ccagattcga cgccagtggt ttctcgcaaa tcacatgaat
    11221 tttatggcta aggaaaagtt gtgtctggga aaagtgcagg gcattcgggc tggcaatata
    11281 caccgcgtca atggcatcgc tttccgccat cgcttccagc gaggtaaaca gatgctcgac
    11341 agaaaaatca ttggcgaagt gctgggcctg ttcaaggctg cgggaatata cggcggttaa
    11401 cttgtattta ccgctctcat gggcggcctc gacgaactgg cgagtgatcc agttcgtacc
    11461 aatcacagcg aaacgtatca taacgcgtgg atactccata aagggattct gtcgtcaatg
    11521 tagcacgcct ttttcatctt cgacgtgcgc agatacgcat tattcttttg ccggacagcc
    11581 atgctcacta aacggcccaa tcatgaccga tggcccgaga tatttcggct ggctatggag
    11641 gattttggcg tcaatcacgg cgctgacacg agcgagtttc tctcgcagtt gagtataaaa
    11701 accgtgcttc atattgaggt caggcgcgtt aaaaccgata gcgtcgatgc tgtactgttt
    11761 tgccagccag atggcgcgtt ggttatgaaa ttcttgcgag ataatggtga tatggttttc
    11821 accaaaaacc tttttggcac gcactaccga atccagcgtt gagaatccgg catagtcgca
    11881 gaagatcact ttcgccggta cgcctttagc gatcaaagcc ttctgcatac ctgatgcttc
    11941 atcgtaattt ttacgtccgt tatcgccact taccagtagc catttcactt ttccggcgtg
    12001 gtacaacgct gctgcggtat caatacgtcg ggtaaagtag cgattacccg gcctggcccc
    12061 cagcaataag cccacgttac gcgccggaac tgcgttgaca tcgctccagg tcagctgctt
    12121 gctggcgttg accattactc gatcggcgat aaaaatggtc gctcctgcca caagtaacag
    12181 taagcaggca atctttagag tccggcgaga aaaacaaata cggagaagaa ggcggggctg
    12241 catggtcgtt atccttaacg aaagggggga ggtcgatttt aggcgctaaa gaaagaaaag
    12301 ataacgcctg acatcatttt cagattttgt cctgcccaag cgacaactgc gttagccaca
    12361 gccgggtgtc gaactccagc tggtggtact gcggctccat atggcagcac aactggtaaa
    12421 acgctttgtt atgctctttc tccttcaggt gcgccagttc gtgcaccacg atcatacgta
    12481 aaaacggttc cggcgcgttg cgaaacacag tagcgacgcg gatctctgct tttgctttca
    12541 gcttgccgcc ctgcacacga gaaacagcgg tatgtaaccc gagcgcgttt tttagcacat
    12601 ggatcttatt gtcatacatc actttattga tcggcggggc attgcgcaga aactgatttt
    12661 ttagatcctg agtatattgc cagagggctt tatcggtggc gtagtcgtgt gttcccggat
    12721 agcgttttgc cagcacatcg cccagacgct gttcgttaat cagcgttcgc acctgggaaa
    12781 gtagctgctc gggataaccc tgaagataag taagattgct cataaccgcc cacgaaaaag
    12841 agaaaaaggg tatactcacg cacccttttc aggggcaacg tcgaaatttt atcattcagg
    12901 agggccgatg agccacttag acaacggttt ccgttcactg acactacaac gttttccggc
    12961 gacggatgac gttaacccgc tacaggcgtg ggaagcggcg gatgaatatt tgctgcaaca
    13021 gttggacgac acagaaatcc gcggcccggt gttgatcctg aatgatgcct ttggtgcgtt
    13081 aagctgcgcg ctggcggaac ataagccgta cagcattggc gactcataca tcagtgaact
    13141 ggcgacgcgc gagaatttac gcctcaacgg gattgatgag tcgagcgtga agtttctcga
    13201 cagcaccgcc gactacccgc aacagccggg tgtggtgctg atcaaagtgc cgaaaacact
    13261 ggcattgctg gaacaacaac tgcgtgcgct gcgcaaagtg gtcacgtcgg atacacgtat
    13321 tattgccggt gccaaagccc gtgacattca cacttccacg ctggaactgt tcgaaaaagt
    13381 gctcggcccg accaccacca cgctggcatg gaaaaaagcg cgcctgatta attgcacgtt
    13441 caatgaaccg ccgctggccg atgcgccgca gaccgttagc tggaagctgg aaggtactga
    13501 ctggactatc cataaccatg cgaatgtctt ctcccgcacc gggcttgata tcggcgcgcg
    13561 cttctttatg caacatctgc cagagaatct cgaaggggag attgtcgatc tcggttgcgg
    13621 taatggcgtt attggtctga cgctgcttga taaaaatccg caggcgaaag tggtgtttgt
    13681 cgatgaatcg ccgatggcgg ttgcttccag ccgtttgaac gttgaaacca acatgccaga
    13741 ggcgttggat cgcagcgagt ttatgatcaa caacgcgctc tccggcgtgg agcctttccg
    13801 ctttaatgct gtgctctgca acccgccgtt tcaccaacaa catgcgctga ccgataacgt
    13861 cgcctgggag atgttccacc atgcccgccg ctgcctgaaa atcaacggcg agctgtatat
    13921 tgttgccaac cgtcacctgg attacttcca taaactgaag aagattttcg gcaactgcac
    13981 caccatcgcc acgaataata aatttgtggt gctgaaagca gtgaagctgg ggcgtcgtcg
    14041 gtaagattgg gagttacctg ccggatgcgg cgttaacgcc ttatccggcc tacgttcagc
    14101 acgttactcc caggccggat aagacgcggc aagcgtcgca tccggcaatg tctgcccagt
    14161 tctggaatcg cgcttccata aaacaaaaaa tccctctcac catcctctac attctcttgt
    14221 ttagcgtttt tctacgttta ttcttccgtc acacagataa attcatccgt tgccaatctg
    14281 tcaacggatg ttatcatatt gccaatttgt caacggacgt gatgaagaat gcacctgata
    14341 actcaaaaag cattgaaaga tgctgcggaa aaatacccgc aacataaaac ggagttggtg
    14401 gctctgggga acatgattgc taagggatat ttcaaaaaac ctgagtcatt aaaagcagta
    14461 ttcccatctc tggataactt caaatatctg gataagcatt atgttttcaa tgttgggggc
    14521 aatgaattac gtgttgtagc aatggtcttt tttgaatcgc aaaagtgcta catacgtgaa
    14581 gttatgacgc ataaagaata cgatttcttt accgctgttc atcgtactaa ggggaaaaaa
    14641 tgattgcgat tgccgacatc ttgcaagcag gagaaaagct aactgcggtg gtaccttttc
    14701 tggcgggtat tcagaacgag gaacaataca cccaggcgct ggaactggta gatcatctgc
    14761 tgctcaacga tcctgaaaac cccttgctgg atctggtgtg tgccaaaata accgcgtggg
    14821 aagaatcagc gcccgaattt gcggaattta atgccatggc tcaagccatg cctggcggta
    14881 tagccgtgat tcgtaccctt atggatcaat atggtttaac ccttgccgat ctgccggaaa
    14941 ttggcagtaa atctatggtg tcacgcgttt tgagcgggaa gaggaaatta acgctggaac
    15001 acgctaaaaa attggcaacg cgattcggca tttctcccgc cttgtttatt gattaacgcg
    15061 acgtgccgga tgcgttgcat ccggcgcttc aaataacgat ttaaatctcc agcgccagcc
    15121 gtgttccctg ggcaattgcc cgtcgtgcgt tcagctccat agccacatcg cagccgccaa
    15181 ttaaatgcac agttttcccg ctatcaatca gcggttgcgc cagcgcgcgg tttggctctt
    15241 gccctgcgca gatcaccaca ttgtccacag ctaatacctg cgtttcgccg ttgatcacca
    15301 catgcagccc gtcatcgtca atcttctgat aacttacgcc tgggatcatt ttcacacccc
    15361 gcgagagcag ggtggtgcga tggatccagc cggtggtttt gcctaacccc tgtcccggtt
    15421 tgctggcttt gcgctggagc atcacaattt gccgtgggct acgcgggatc tgcattccct
    15481 gcgggcttaa gccaccagcc tgttgtaggc tgctgtcgat cccccactca ttacagaacc
    15541 tggcgatatt ctggctggtg gattcgcccg gctgacttaa atacatcgcc gtatcaaaac
    15601 caatcccgcc acaaccgatg atggcaacct tgttgcctac cggcgctttg tcgcgcagta
    15661 catcgagata actcaatacc ttcggatgat cgatcccgtc gataggcgga acgcgcggca
    15721 cgatcccact ggcgaggatc gtttcatcga acgcctgtaa ctgatccgcc gtcacggtgt
    15781 gattgagttt cagcgtcacg cccgtcactt cgatcatccg gtggtaatag cgcagcgttt
    15841 cgtaaaactc ctctttgccg gggatctgtt tggcgatatt aaactgcccg ccgctctcgc
    15901 tgtgggcatc aaacaatgtt acctgatgcc cacgcgccgc cgcgttaatg gcaaacgcca
    15961 gcccagcagg tcccgcaccg accaccgcca gatttttttt ctgcacggca ggaaggattg
    16021 gcattttggt ttcgtggcag gcgcgaggat tcaccaggca cgaggtgact ttgccaacga
    16081 agatctgatc gaggcaggcc tgattgcagc caatgcaggt gttgatctca tcggctcgcc
    16141 ccgattgcgc ttttgacagc agttccgcat cagcaagaaa cggtcgtgcc atcgatacca
    16201 tatcggcatc gccgcgcgag agaatatcgt cggcaacctg cggatcgtta atccggttgg
    16261 tggttaccag cggcagcgag acgtggcctt tcagtttgcg cgtgacccag ctaaatgcgc
    16321 cgcgcggcac gggcgtggca atggtcggaa tacgtgcttc atgccagcca atgccggtgt
    16381 tgataatggt cgcgcccgcc gcttcaatgg cctgcgccag ctctaccgtt tcggcaaaag
    16441 tcccgccgtc ttcgaccagg tcgagcatcg acagtcggta gataataatg aagtcgttgc
    16501 cgacgcgttc gcgcaccgca cgcactactt ctacggcaaa tcgcatccgg ttgcggtaat
    16561 cgccgcccca ctggtcacta cgctgattgg tgtgcagcgt cagaaattcg ttgatcaaat
    16621 acccttcgga ccccattacc tctacaccgt cgtatcctgc ctcccgtgcc agttgcgcgc
    16681 agtgggcgaa atcgtcgatc agttgcagga tctcttcatg ggtgagttcg tgaggaacga
    16741 aacggttgat gggggcctgc aatgcggagg gagcgaccag atgcggttgg tagctgtagc
    16801 gcccggtatg caaaatttgc agggctattt tgccgccttc ctgatgtacc gcttcggtaa
    16861 tggtgcgatg gtgtgggatc tggctggcgt cgttgagcat tgcgccacct tccatgccaa
    16921 cgcctgttaa atctggtgca atgccgccgc tgacaatcag cgccacgccg tgacgggcgc
    16981 gttcggcata aaacgctgcc agccgctcgg caccgtccgg gtattcctcc agcccggtgt
    17041 gcattgagcc catcaacacg cggtttttta acgtggtaaa acctaaatcc agcggggcga
    17101 acagcgacgg gtagctcata aaattgtcca gtatgtaaaa taattgttat gtggtcggat
    17161 gagttctaat ttagccgtcg ccggagaaaa gggaaaaggg gagtgcgggg tttgtgatgg
    17221 gattcaaaaa gaatacgccc ggtggtaacg ccggatgcga cgcttgccgt gttttatccg
    17281 gccaggagga ttgcacatgt agggcggata aggcgtttac gccgcatccg ccagcagttg
    17341 caaattattg cttacggaaa aaatcgttat acaacatata caaatgcgcg gcactccagg
    17401 agaaatttgg tgcagccttg ctgcgcgcca gtcagcgggt tgtaattctc ctgaattggg
    17461 ccatcggcgg ttaatccttt ggcgtgccgg aagaacgtat ccgccagctt cagggcatca
    17521 tcacgataac cgtaacgctc catccctttc agagcaaacc agaactgatc cacccatacg
    17581 cgcccgcgcc agtagatatc agcgccaaaa gccggattgg ttaacgccgc cgttcccagc
    17641 gggacaaagg tgttgaactc tttaggatcg agcatcacct tcaccaccgc gtcggcattg
    17701 gcctgcgttg ccgcaccgtt aaacagcggc gaccagcctt ccggcccttt accgcgctca
    17761 acaatcggtt tgcccgcgca gccgttcgcc agtggtttat cttcaatacg tacgtcatag
    17821 tagaactggg tagtcgggtc gaacatacag gtgttgatgt agtccgcgag ctgctgtgcc
    17881 aactggcgat agcgtttggc ctcttctggc ttaccgagga tggttgccat ctccgccaga
    17941 taatggttat cgctgtacat atagctggcc tgatccaccg actcctgcaa tagcgagtag
    18001 cccagcaacg ttccgtcctg actgcggttt tcggcgaatt tcaccgtcca gtcgctacgt
    18061 ttgccaccat tagcgacata tttatccaac tgttctttgt cgataaaacc aaagacggcg
    18121 gcatcatcac gccccgattc ccacgacgca gcgacctgtg ccggaatttc cagactgtcg
    18181 tactggcctt tctccaccac gcgggcgtag ttgttcagcc cagactgcgt ctcttctttg
    18241 ttgcctttct tcaccgcaaa cagcatctcg ccgctctcag tgttgtgggc tttgtcgcgg
    18301 gtcgcgccat attccggcac gccgttgccg ttgtgatcgc ggttacgtaa ccaccagtcg
    18361 tgataggcga ccagtttcgg gtacatctct gccaaccagg ctttatcctg ggtgacgttg
    18421 tacacttcta tcaccgacca ggcggcaagg ctgggtttgg tattacgttc gttccagttg
    18481 ccgccatcgc caccacgctc ggggctaagg ttccacgcta tcaggtcggg gacaaagccc
    18541 acatcctgcg gacgcacgcg gtcgccaggc tggatctgcc aggagaagac cgcgcggata
    18601 ttctctttgg cgatgtccgg attgaaatgc gccatcgcaa acgcctgctt ctaggtatcc
    18661 cacggccagg tctgattgcc ggagaaccag cgcccggtca ccgacggtgt aacggtgtta
    18721 aatttcaccg caccgcccgg tgagcgccag ttaccgttga gcgtttcgat cgctttcacc
    18781 gcgacgcgcg tctgttccgg cgtcgcatcc ggattggtta accctttctt caggtattct
    18841 tcccagcgtt gctgcgaggc ggtgagataa aacgccggac gcgccagaat atcgcggatc
    18901 tgcatttgct ctttgctaac ttcctgagcg gtcagcagat gggaataggt ggtgtagagc
    18961 gtggtcgaac cgttgatatg cgccttactg gtaaagcgat taccgttgat ttcagtctgc
    19021 accggcaggg atttatgcac ctgatattcc gattcgccgg aggtcagcag atcccaggtg
    19081 gcgcgcactt tgccaaaggt gactttcagg ccatcacggg tggcgctgat tttgcgctga
    19141 tagtcagggt attcgccagc aatggtttta tcggaaagcg gtttcccttc tttcgcttcc
    19201 agtttttcca gcagttcgcc gtcccacacc agatccagcg gtttattgct ggtgattttg
    19261 gtttccagta gtgacgtgcg cggcgtggcg aagcgcagag tcatttcgac ttgcacatct
    19321 tttgctgtca gtttttgcac cagcgcaccg ggaatactgt atgcctccag cgtaaagtcg
    19381 actttcttgc cgtcctgcca gacggtcagg cggtcgaaat tgctggccat aaagttgatg
    19441 tactcttccg tcagcagcgc aacgcccgga aagccgccca tggtgttagg gccgtctggc
    19501 aacagatgac catgccaggc tccgagatcg aaaaacggat taaaacgctg gtgatcgtcg
    19561 taatcgtaat ccttcatgta ctgcggcgcg ccagtacggt taatcacgtt tttataattg
    19621 tcggcgttag cggcatgggc ggaaaaactt atcagcagag cgcaggttac tggcgttaaa
    19681 atagttttta ttttcatgaa attacagtcc ttattaccag tagaaatatt gcatcaggaa
    19741 gatttggtcg ctgggatcgt gagaggcatt tttcatgtag aagcggctat caaaagccgt
    19801 ggcgaaacgt ccgccgccgt attcgtacca gataccaaac tgcatgaaag aggtggtttc
    19861 atcgtcagcg tggtcagggc gatgtttggc gtaggtgtaa gcggtggaga gataaacgcc
    19921 ctttaacgct tcatacatgg cgctcaccat catcccactt tcacttcccg gtacgtccag
    19981 cccgtcggcg cgtccggtat ggtgccaggc gcgagcggca aagttaggag ttagcaggcc
    20041 cgtgagatag agctgtcctg tgccgtcggt gatttccaga ccgttcatcc aggtgatgcc
    20101 ttcggcaagg tcatactgga tgtaaccgtt catcattgcc ggataggtgt atttgtcgtc
    20161 atagcgatcg tatttgccaa aatgtagcca ggctttgctt tcatcatgat gtccggcagg
    20221 agtcgcggtg acgctataac gaaagttgcc gcttaagttc tgtaccttca gcgcagtcat
    20281 aatgtcgcgg gtattgggaa tgacgtagcc cagatcgggg gtgaaatcgc cccaccattg
    20341 caggtcatcc agcgaggaat cctgacgcag actgagcatg atttcggtgc catcattggt
    20401 acgataaccg ccatagaaac ggttaattcc gccctcgaaa ccaccccagc tatggtctgg
    20461 cac
//
LOCUS       DAJVUJ010000065        20405 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.065, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000065 DAJVUJ010000000
VERSION     DAJVUJ010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20405
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.065"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            127..942
                     /gene="mepH"
                     /locus_tag="OQQ48_002140"
     CDS             127..942
                     /gene="mepH"
                     /locus_tag="OQQ48_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HCS1952907.1"
                     /translation="MAQINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            1070..1651
                     /gene="sodB"
                     /locus_tag="OQQ48_002141"
     CDS             1070..1651
                     /gene="sodB"
                     /locus_tag="OQQ48_002141"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HCS1952908.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(1797..2972)
                     /locus_tag="OQQ48_002142"
     CDS             complement(1797..2972)
                     /locus_tag="OQQ48_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1952909.1"
                     /translation="MKINYPLLALALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAA
                     GMLISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSR
                     ILTSLNHGAFFGLGSVVAASVEPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGW
                     RMSFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGA
                     MFTLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYPGGKLADRSVNGTLKGFLL
                     LLMVIMLAIPFLARNEFGAAISMVVWGAAAFAVVPPLQMRVMRVASEAPGLSSSVNIG
                     AFNLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(3138..3227)
                     /gene="ynhF"
                     /locus_tag="OQQ48_002143"
     CDS             complement(3138..3227)
                     /gene="ynhF"
                     /locus_tag="OQQ48_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HCS1952910.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            3526..4551
                     /gene="purR"
                     /locus_tag="OQQ48_002144"
     CDS             3526..4551
                     /gene="purR"
                     /locus_tag="OQQ48_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HCS1952911.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(4548..5480)
                     /locus_tag="OQQ48_002145"
     CDS             complement(4548..5480)
                     /locus_tag="OQQ48_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1952912.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            5593..6804
                     /locus_tag="OQQ48_002146"
     CDS             5593..6804
                     /locus_tag="OQQ48_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HCS1952913.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKAGNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEAGCQNH
                     GNAEVAHSESH"
     gene            7095..8243
                     /gene="cfa"
                     /locus_tag="OQQ48_002147"
     CDS             7095..8243
                     /gene="cfa"
                     /locus_tag="OQQ48_002147"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HCS1952914.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSMVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(8283..8924)
                     /gene="ribE"
                     /locus_tag="OQQ48_002148"
     CDS             complement(8283..8924)
                     /gene="ribE"
                     /locus_tag="OQQ48_002148"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HCS1952915.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            9139..10512
                     /gene="mdtK"
                     /locus_tag="OQQ48_002149"
     CDS             9139..10512
                     /gene="mdtK"
                     /locus_tag="OQQ48_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HCS1952916.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSVI
                     ILQRASR"
     gene            complement(10553..11809)
                     /locus_tag="OQQ48_002150"
     CDS             complement(10553..11809)
                     /locus_tag="OQQ48_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HCS1952917.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDGVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIHVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            12117..12193
                     /locus_tag="OQQ48_002151"
     tRNA            12117..12193
                     /locus_tag="OQQ48_002151"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12151..12153,aa:Val,seq:gac)
     gene            12198..12274
                     /locus_tag="OQQ48_002152"
     tRNA            12198..12274
                     /locus_tag="OQQ48_002152"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12232..12234,aa:Val,seq:gac)
     gene            12382..12687
                     /locus_tag="OQQ48_002153"
     CDS             12382..12687
                     /locus_tag="OQQ48_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HCS1952918.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            12813..14417
                     /locus_tag="OQQ48_002154"
     CDS             12813..14417
                     /locus_tag="OQQ48_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HCS1952919.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFNRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EEGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFVQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYEMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(14429..15241)
                     /locus_tag="OQQ48_002155"
     CDS             complement(14429..15241)
                     /locus_tag="OQQ48_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1952920.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFRRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIAIAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGSGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(15245..16030)
                     /locus_tag="OQQ48_002156"
     CDS             complement(15245..16030)
                     /locus_tag="OQQ48_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HCS1952921.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(16027..16695)
                     /locus_tag="OQQ48_002157"
     CDS             complement(16027..16695)
                     /locus_tag="OQQ48_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HCS1952922.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(16759..17397)
                     /locus_tag="OQQ48_002158"
     CDS             complement(16759..17397)
                     /locus_tag="OQQ48_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HCS1952923.1"
                     /translation="MNHRDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARAVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAELYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(17410..19512)
                     /locus_tag="OQQ48_002159"
     CDS             complement(17410..19512)
                     /locus_tag="OQQ48_002159"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HCS1952924.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKCVLPAEEYAEIHWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGSPTRETLQR
                     LGLEDIAADLAAHNLLPV"
     gene            complement(19482..20159)
                     /locus_tag="OQQ48_002160"
     CDS             complement(19482..20159)
                     /locus_tag="OQQ48_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HCS1952925.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITVRILQRKKSWL
                     TVGQVIY"
BASE COUNT         5110 a         5311 c         4764 g         5220 t
ORIGIN      
        1 tcgccaacga aacgtatttt ttaacaataa aagctattaa ctttctcttc ttctatgcat
       61 tagaatcatc aagttttgta aatcagacgc aggcatgata gacctgcctt aacagaggga
      121 cgctcagtgg cgcagataaa ccgtatttcg atcacgctct gtgctttact ttttaccacc
      181 ctgcctttaa cgcctatggc ccatgcttca aagcaagcca gggagagttc tgctaccact
      241 catatcacca aaaaagcaga taaaaagaaa agcacggcaa ccaccaaaaa gacccagaaa
      301 acagcgaaaa aagccgccag taaaagtacg accaaaagca aaaccgcttc ttccgttaaa
      361 aaatcttcca ttaccgcttc taaaaacgcc aaaactcgca gcaaacacgc cgtcaataaa
      421 acggcctcag ccagctttac cgaaaagtgt accaaacgta agggctataa atcgcattgt
      481 gtgaaagtca aaaatgccgc gtcaggaact cttgccgacg cgcacaaagc gaaggtgcaa
      541 aaagctacga aagtggcaat gaataaactg atgcagcaaa ttggtaagcc atatcgttgg
      601 ggtggcagct caccgcgtac cggttttgat tgcagcggcc tggtttatta cgcttataaa
      661 gatttggtga aaattcgtat tccgcgtacg gcgaatgaaa tgtatcacct gcgtgatgca
      721 gcgccaatcg aacgtagtga actgaaaaac ggcgacctgg tctttttccg tactcaggga
      781 cgcggcacag ccgatcatgt cggcgtgtat gtcggcaacg gcaaatttat tcagtcaccg
      841 cgcacaggtc aggaaattca aatcacttct ctcagtgaag actactggca gcgccactat
      901 gttggcgctc gtcgggtaat gaccccaaaa acacttcgct aaaactttac cctgttgtta
      961 cggcaacagg gtaagttcat cttttgtctc accttttaat ttgctaccct atccatacgc
     1021 acaataaggc tattgtacgt atgcaaatta ataataaagg agagtagcaa tgtcattcga
     1081 attacctgca ctaccatatg ctaaagatgc tctggcaccg cacatttctg cggaaactat
     1141 cgagtatcac tacggcaagc atcatcagac ttatgtcact aacctgaaca acctgattaa
     1201 aggtaccgcg tttgaaggta aatcactgga agagattatt cgcagctctg aaggtggcgt
     1261 attcaacaac gcagctcagg tctggaacca tactttctac tggaactgcc tggcaccgaa
     1321 cgccggtggc gaaccgactg gaaaagtcgc tgaagctatc gccgcatctt ttggcagctt
     1381 tgccgatttc aaagcgcagt ttactgatgc agcgatcaaa aactttggtt ctggctggac
     1441 ctggctggtg aaaaacagcg atggcaaact ggctatcgtt tcaacctcta acgcgggtac
     1501 tccgctgacc accgatgcga ctccgctgct gaccgttgat gtctgggaac acgcttatta
     1561 catcgactat cgcaatgcac gtcctggcta tctggagcac ttctgggcgc tggtgaactg
     1621 ggaattcgta gcgaaaaatc tcgctgcata ataactgatg gcaaatgcag cattgcctga
     1681 tgcgcttcgt ttaccatgcc aacgcggatg aggcgttaac agcgcattcg gcaagataaa
     1741 tcgcacgttg tcagcaactg taacgcagaa ggttatcctt ctgcgttttt gtttaattag
     1801 ctgttagcaa cgcaaactgt ttcaggttgt tttctggctg acataaacac cagcaataat
     1861 gccagtcccg cgacaatcgc tcccatcacc ggcacaaagc tgtatcccag cccagcggaa
     1921 attaccgcac caccagcagc tgctcccagc gcatttccaa gattaaaggc accaatattg
     1981 actgatgaag acagacctgg cgcttcactg gcgacacgca tcacgcgcat ctgtaacggc
     2041 ggtacgaccg caaaggctgc tgcgccccac accaccatgc taatagctgc gccgaactca
     2101 ttgcgggcca ggaacgggat tgccagcata atcaccatca acaacaacaa aaagcctttc
     2161 aacgtgccgt taactgaacg atctgccagt ttgccgccgg gatagttacc gatagagaat
     2221 ccgacaccaa tcagcaccag cattgccgtg acgaacaccg gtgttgcgtg ggtaatactt
     2281 tgcagtaccg gagagatata ggtgtagaga gtaaacattg caccagctcc cagtaccgtc
     2341 gtcagcaatg cagacaacac ctgcggacgc attaataccg ccagctcttt tttcacttca
     2401 ggtcgtgccc ctgcaccacc tttaggtaat gagaagaaca gacttaccat tgaaatcact
     2461 cccagccccg ccgttgccag aaatgacatc cgccagccga tggtttcacc caaccaggtc
     2521 gccgccggca cgccaccgat atttgccagg gttaacccca taaacatagt ggcaactgcg
     2581 ctggcctgtt tatgttttgg ctccacgctt gcggccacga ctgaacccaa accaaaaaat
     2641 gctccgtgat tcaggctggt caaaatgcgt gaaagcatca gggtcatata atccggcgcg
     2701 atggcggaaa gtacgttgcc gagcgtgaaa attgccatca ggaaaatcaa cgcactgcgg
     2761 cgggcacgat gagaaagtag aagcgtcatc agcggcgcgc caaccattac gccaactgca
     2821 taggcactga ttaacattcc ggcagcggga atcgagacat ccacaccgcg cgcaatgacg
     2881 ggcaacaagc ccattggcga gaactccgtt gtcccgatac caaacgcgcc aatcgccagc
     2941 gccagcgcca gcaacggata gttaattttc atgccttatc tccacctctt cgcgtcatta
     3001 cgcgatattc attaaagtgg cgaaagcatg acagcaatca caaaaaaatg aaaataacaa
     3061 aaagagaaaa cacttttgcc attttgctaa caaacaggaa ggagatgcga gggagaacgc
     3121 gctccctcga gaggaaatca gtgcagcgcg gcagtcaaac ccacggccac gatcaaaccg
     3181 aggacgataa tcgttgttac cagtgaaaat ttaaggtcgg tgctcatcaa gttttctcct
     3241 tttttattac cacacaaata gtgatattac gcatttttac acactgtgat gaaaaaatct
     3301 cccgtcattt ataatgataa gtgtttttac cacttcccct tttcgtcaag atcggccaaa
     3361 attccacgct tacactattt gcgtactggc cattgacccc ttcctgacgc tccgtgtcgt
     3421 ttttccggcg taccgcaaca cttttgttgt gcgtaaggtg tgtaaaggca aacgtttacc
     3481 ttgcgatttt gcaggagctg aagttagggt ctggagtgaa atggaatggc aacaataaaa
     3541 gatgtagcga aacgagcaaa cgtttccact acaactgtgt cacacgtaat caacaaaaca
     3601 cgtttcgtcg ctgaagaaac gcgcaacgcc gtgtgggcag cgattaaaga attacactac
     3661 tcccctagcg cggtggcgcg tagcctgaag gttaaccaca ccaagtctat cggtttgctg
     3721 gcgaccagca gcgaagcggc ctattttgcc gagatcattg aagcggttga aaaaaattgc
     3781 ttccagaaag gttacaccct gattctgggt aatgcgtgga acaatcttga gaaacagcgg
     3841 gcttatctgt cgatgatggc gcaaaaacgc gtcgatggtc tgctggtgat gtgttctgag
     3901 tacccagagc cgttgctggc gatgctggaa gagtatcgcc atatcccaat ggtggtgatg
     3961 gactggggtg aagcaaaagc tgacttcacc gatgcggtca ttgataacgc gttcgaaggc
     4021 ggctacatgg ccgggcgtta tctgattgaa cgcggtcacc gcgaaatcgg cgtcatcccc
     4081 ggcccgctgg aacgtaacac cggcgcaggc cgccttgccg gttttatgaa ggcgatggaa
     4141 gaagcgatga tcaaggtgcc ggaaagctgg attgttcagg gtgactttga acccgaatct
     4201 ggttatcgcg ccatgcagca aatactgtcg cagccgcatc gccctactgc cgtcttctgt
     4261 ggtggcgata tcatggcaat gggcgcactt tgtgctgctg atgaaatggg tctgcgcgtc
     4321 ccgcaggatg tttcgctgat cggttatgat aacgtgcgca acgcccgcta ttttacgccg
     4381 gcgctgacca cgatccacca gccaaaagat tcgctgggtg aaacagcgtt caacatgctg
     4441 ttggatcgta tcgtcaacaa acgtgaagaa ccgcagtcca ttgaagtgca tccgcgcttg
     4501 attgaacgcc gctccgtggc tgacggcccg ttccgcgact atcgtcgtta atcacccgtt
     4561 gcgggagtct cttccggctc ccgcagccac tccttattca gcgtctcact atcgccgaga
     4621 tactcaagca gccaggttaa cgcaggcgac atatcatttt gctgccatgt cagacaacat
     4681 gccgaatccg ggaaggggtt ttccaattca agtgctaccc acttcccctc attaagccac
     4741 ggtttggcga aatgtgttgg caccatccct atgcataatc ctgccgagat acaggttgcc
     4801 gatgattccc agtcaggcac gacaactctt ttttggttat ccagtaacca ggtaatacgt
     4861 ttaggtagcg ttcgcgaggt gtcttcgcgc accaacgacg gccagttgcg caacgtatca
     4921 tcgctgaatg ggccatccat caacgccagc gggtggtggc tggcaacaac gcaactccag
     4981 cttagcatcc ccatatcccg gaaggcataa cgaccgccta ccggaatcgc gcgtgttgcg
     5041 ccaatcgcca gttccacgcg cccgtcggaa agcgcatccc agacaccgtt gaacacttcc
     5101 tgaaagacaa gaagttcgac atcatcaaaa tggcgataaa aatcaacgat catctgccgt
     5161 gtacgttctg gcctgacaat attatccact gcgatagcta actgaccgcg ccagccgttc
     5221 gctatctgct gacattgctg gcgggtgatc tgcatttttt tgacaacaga gcgcccttct
     5281 ttgagaaacc acgctccagc agcggtcagc tccacatcac ggtgccgtcg ttcaaagagc
     5341 ggcaccgcca gccactcttc cagctgacgc acggtatagc tgaccgcaga aggaacgcga
     5401 tgcagctcct gtgccgcagc gctaaaacta ccattacgcg ctaccgcatc aacaacttcg
     5461 agtgaatatt ctgaccacat agtctgcctg caaaattttt gaaatcagtc atcaaatatt
     5521 accgtttcac aacactaatt tcactcccta cactttgcgg cggtgtttaa ttgagagatt
     5581 tagagaatat acatgcaacc tgggaaaaga tttttagtct ggctggcggg tttgagcgta
     5641 ctcggttttc tggcaaccga tatgtatctg cctgctttcg ccgccataca ggccgacctg
     5701 caaacgcctg cgtctgctgt cagtgccagc cttagtctgt tccttgccgg ttttgccgca
     5761 gcccagcttc tgtgggggcc actctccgac cgttatggtc gtaaaccggt gttattaatc
     5821 ggcctgacaa tttttgcgtt aggtagtctg gggatgctgt gggtagaaaa cgccgctacg
     5881 ctgctggtat tgcgttttgt acaggctgtg ggtgtctgcg ccgcggcggt tatctggcaa
     5941 gcgttagtga cggattatta tccttcacag aaagttaacc gaatttttgc gaccatcatg
     6001 ccgctggtgg gtctatctcc ggccctggct cctctgttag gaagctggct gctggtccat
     6061 ttttcctggc aggcgatttt cgccaccctg tttgccatta ccgtggtgct gattctgcct
     6121 attttctggc tcaaacccac gacgaaggcc ggtaacaata gtcaggatgg tctgaccttt
     6181 accgacctgc tacgttctaa aacctatcgc ggcaacgtgc tgatatacgc ggcctgttca
     6241 gccagttttt ttgcatggct gaccggttca ccgttcatcc ttagtgaaat gggctacagc
     6301 ccggcagtta ttggtttaag ttatgtcccg caaactatcg cgtttctgat tggtggttat
     6361 ggctgtcgcg ccgcgctgca gaaatggcaa ggcaagcagt tattaccgtg gttgctggtg
     6421 ctgtttgctg tcagcgtcat tgcgacctgg gctgcgggct tcattagcca tgtgtcgctg
     6481 gtcgaaatcc tgatcccatt ctgtgtgatg gcgattgcca atggcgcgat ctaccctatt
     6541 gttgtcgccc aggcgctgcg tcccttccca cacgcaactg gtcgcgccgc agcgttgcag
     6601 aacactctac aactgggtct gtgcttcctc gcaagtctgg tagtttcctg gcttattagt
     6661 atcagcacgc cattgctcac caccaccagc gtgatgttat caacagtagt gctggtcgcg
     6721 ctgggttaca tgatgcaacg ttgtgaagaa gctggctgcc agaatcatgg caatgccgaa
     6781 gtcgctcata gcgaatcaca ctgatctata tcgatatact tatacttagg ctgctaacaa
     6841 aattttgttg tatgattgaa attagcggcc tatactaatt tcgagttgtt aaagctacga
     6901 taaatattat gtttttacgg ggacaggatc gttcccgact cactatggat ggtcatttcg
     6961 gcaagggttc ctcctttccc tctgttctac gtcggattat agactcgcgg ttttttctgc
     7021 gagatttctc acaaagccca aaaagcgtct acgctgtttt aaggttctga tcaccgacca
     7081 gtgatggaga aactatgagt tcatcgtgta tagaagaagt cagcgtaccg gatgacaact
     7141 ggtaccgtat cgccaacgaa ttacttagcc gtgccggtat agccattaac ggttctgccc
     7201 cggcggatat tcgtgtgaaa aaccccgatt tttttaaacg cgttctgcaa gaaggctctt
     7261 tggggttagg cgaaagttat atggatggct ggtgggaatg tgaccgactg gatatgtttt
     7321 ttagcaaagt cttacgcgca ggtctcgaga accaactccc ccatcatttc aaagacacgc
     7381 tgcgtattgc cggcgctcgt ctcttcaatc tgcagagtaa aaaacgtgcc tggatagtcg
     7441 gcaaagagca ttacgatttg ggtaatgact tgttcagccg catgcttgat cccttcatgc
     7501 aatattcctg cgcttactgg aaagatgccg ataatctgga atctgcccag caggcgaagc
     7561 tcaaaatgat ttgtgaaaaa ttgcagttaa aaccagggat gcgcgtactg gatattggct
     7621 gcggctgggg cggactggca cactacatgg catctaatta tgacgtaagc atggtgggcg
     7681 tcaccatttc tgccgaacag caaaaaatgg ctcaggaacg ctgtgaaggc ctggatgtca
     7741 ccattttgct gcaagattat cgtgacctga acgaccagtt tgatcgtatt gtttctgtgg
     7801 ggatgttcga gcacgtcgga ccgaaaaatt acgataccta ttttgcggtg gtggatcgta
     7861 atttgaaacc ggaaggcata ttcctgctcc atactatcgg ttcgaaaaaa accgatctga
     7921 atgttgatcc ctggattaat aaatatattt ttccgaacgg ttgcctgccc tctgtacgcc
     7981 agattgctca gtccagcgaa ccccactttg tgatggaaga ctggcataac ttcggtgctg
     8041 attacgatac tacgttgatg gcgtggtatg aacgattcct cgccgcatgg ccagaaattg
     8101 cggataacta cagtgaacgc tttaaacgga tgtttaccta ttatctgaat gcctgcgcag
     8161 gtgctttccg cgcccgtgat attcagctct ggcaggtcgt gttctcacgc ggtgttgaaa
     8221 acggccttcg agtcgctcgc taaaggctat tctatcgccc cctctccggg ggcgatttca
     8281 gatcaggctt ctgtacctgg ttgattcatg gcattttctc gtgccgccag cacacgttct
     8341 accgtatcta ccactgcctg agtttgtgga tcaatttcaa tgttgacgcg tgcgccgagt
     8401 tttttcttcc caagagtcgt gcgttccaga gtttccggaa ttaaatggac gcaaaaacgc
     8461 gttggcgtga cttcgccgac ggtcaggcta ataccgtcga tgccaataaa tcctttgtac
     8521 aggatatatt tcatcaactg actatcctgg actttaaacc agatctggcg attattttcc
     8581 gaggttaata ttttcgccac ttcagcggtg gtcataatat gacctgacat taagtgtccg
     8641 ccaatttcat cactgaattt cgccgcacgc tcaacgttta cccaatcccc cacttttaaa
     8701 tcgccaagat tggtaatgcg taacgtttct ttcatcaggt caaaactgac atggttgccg
     8761 ttaatttccg tcacggtcag gcagcaaccg ttatgcgcca cggaagcacc ggtttccagg
     8821 ccgtccagca tgtggtcggg taactccacc acatgcgtac gaaaatttgg tttctcgtca
     8881 atcgacacca gttttgcggt gccctgtaca atccccgtaa acatacttac aactcctgaa
     8941 atcagttaag acattctgtt cagcacaata gcaggtggca aacgccctta ccagtgaagg
     9001 ggtaagaatg actatttttt cactggagaa ttaataaatc ctcgctacaa tagactgaat
     9061 ttcccctgct tcttcttttt gctgcccatt caggcggctt tttagtctct catataacta
     9121 caaataaaag gtgttcacgt gcagaagtat atcagtgaag cgcgtctgtt attagcatta
     9181 gcaatcccgg tgattctcgc gcaaatcgcc caaactgcga tgggttttgt cgataccgtg
     9241 atggcgggcg gctatagtgc caccgacatg gcggcggtcg ctatcggtac ttctatctgg
     9301 cttccggcga tcctctttgg tcacggactg ctgctggcat taacgccggt tatcgcgcaa
     9361 ttaaatggtt ccggtcgacg tgagcgcatt gcgcatcagg tgcgacaagg tttctggctg
     9421 gcaggttttg tttccgttct cattatgctg gtgctgtgga atgcaggtta cattatccgc
     9481 tccatggaaa acatcgatcc ggctctggcg gacaaagccg tgggttatct gcgtgcgttg
     9541 ttgtggggcg cgccgggcta tctgttcttc caggttgccc gtaaccaatg tgaaggtctg
     9601 gcaaaaacca agccgggtat ggtaatgggc tttatcggcc tgctggtgaa cattccggtg
     9661 aactatatct ttatttatgg tcatttcggt atgcctgagc tcggtggcgt tggttgtggc
     9721 gtggctactg cggcggtgta ttgggtcatg ttccttgcca tggtttctta cattaaacgc
     9781 gcccgctcca tgcgcgatat tcgtaacgaa aaaggcaccg cgaaacccga tcctgcggtt
     9841 atgaaacgac tgattcaact cggtttaccg attgcgctgg cactattctt tgaagtgaca
     9901 ctgtttgccg tcgtggctct gttagtgtct ccgctcggta ttgttgatgt cgcaggacac
     9961 cagattgccc tgaactttag ttcactgatg tttgtgcttc caatgtcgct ggcggcagcg
    10021 gtaactatcc gcgtaggtta tcgtctgggt cagggctcaa cgctggatgc gcaaaccgct
    10081 gcgcggaccg ggcttatggt gggtgtctgt atggcaaccc tgacggccat tttcacggtt
    10141 tcactccggg agcaaatcgc cctgttgtac aacgacaatc ccgaggttgt aacgctggct
    10201 gcgcatttga tgttgctggc ggcggtatat cagatttctg actcaatcca ggtgattggc
    10261 agtgggattt tgcgtggtta taaagatacg cgttccattt tctatatcac ctttacagct
    10321 tactgggtgc tgggcttgcc aagcggctat attctggcac tgaccgatct ggtcgttgaa
    10381 cctatggggc cagcaggctt ctggataggc tttattatcg gcctgacatc ggcagccatt
    10441 atgatgatgt tgcgtatgcg gttcctgcaa cgtctgccgt cagttatcat tctgcaacga
    10501 gcatcccgct aataaagaca aggcgcaacc ttcacgggtt gcgcctgtgt ttttacgcag
    10561 gctggagcgt tgcgccaatc ccgtcttcgt ctggctgtaa tttcagagcg ttgcacagag
    10621 tttgccgact ggcatctgtt atctcaacgt gtatatcacc ggtaacttcc cctttcacat
    10681 tgatgaagtc aggtgagatt tctttcgcac tggtttgcag gcaaatcgct ccctctccgc
    10741 gcaggcgttt cactttcagt tgcacatgcg ttgccgcacc agtgatcagc cacagttttc
    10801 cgccattcag attgatgtta ccaacaataa aagtgacacc gtcacctttt accgtactgc
    10861 cgtcgagaat caacacaccg ccctgctgta cagtaatcgt tgattgttgc agatctgcgc
    10921 catagccaat aatcattgaa gcgccgtcca gtaatgcaac actcccggtc agttcaaccg
    10981 gtgcgcgatc ttcctctacg acaaaatttt cgtccgcgct ggtgggtgac aacaggatca
    11041 ctgttccacc ttgtattacc gcattttcca gcgtaccggc atgcacttca gcccgaccac
    11101 ccacattcac ggacaggttt tgcgtcttac cggaactgta gagctgaagg ccatccgtcc
    11161 ccagacgata aatggatcca ttctcaacaa tggtgtcaga gtcttcacca ccgagattat
    11221 tcatggtgcc accagcaagc aacgtatcac tggctttccc ggccagcata aaaacgccgc
    11281 cctgattaat ctcacaattg ctggcttccc cacctggata caccaacaat tcaccacctt
    11341 catctaccac gaccgctgag gttgtcccat taaccaacag gccgccctct tgatcgagaa
    11401 tgattttttc cgcgcgatgt ccttccagta cacgcaggtt accgccattt tccagcaaca
    11461 aaccgcaggc ataaccctga tcaacgctaa attcaccttc gggatggcgg ccattaaccg
    11521 tagcgagcgt agagagacta attgcgcctc cctcggactg aacaatatct gtggcaatgg
    11581 caccgtcgct gaccgatagc gtgccattgt taccgtactg tgtgcatttg gcgatcccac
    11641 cggttgccac tttcaaccag ccagcccccg tgactacggt attttcggct cttccgccag
    11701 cttcaacaat caactcgcct tcagtaagtt gcgtcgcgcc aatgacctcg ccggtcttaa
    11761 caatttgcac attcccgtcg ctcatcaagt ttttcgcatc agatcccatg atttattcct
    11821 ttgctgcatc tgtgtgcctt tattgctacc taaatgtaaa ggctacggag gatttatcca
    11881 cgacagattt gagatggtgg caaacaactc tgtttaaact ctgatacacg aattattggg
    11941 ttgtatcaga tgtaaatgcg atcctgaata aaaatcgccc ttgcaaatca acaaaatata
    12001 gccaatttgc ttaaaagctc tgcaaacggt cgaaatagcg cagaaaatta cgttttgcct
    12061 cttgccacct tcccactctg ccgctaatat tcgtccccgt tgtcacctac aacgttgcgt
    12121 tcatagctca gttggttaga gcaccacctt gacatggtgg gggtcgttgg ttcgagtcca
    12181 attgaacgca ccatcctgcg tccgtagctc agttggttag agcaccacct tgacatggtg
    12241 ggggtcggtg gttcgagtcc actcggacgc accagatttt cttaatctgg tcttctcctt
    12301 tttccctctg tttcttctct gtatccaata cgttaaaaga tttacactgt cttcgtatgc
    12361 gttatcagaa ggagaatcgc tatggcaact ttgttacaac ttcattttgc ttttaatggc
    12421 ccatttggcg acgcaatggc tgagcagctt aaaccgcttg ctgagtcgat taatcaggaa
    12481 cctggttttc tgtggaaggt atggacagaa agtgaaaaga accacgaagc cggtgggatc
    12541 tatcttttta ctgatgaaaa aagcgccctt gcctatctgg aaaaacatac tgcccgactt
    12601 aaaaatctcg gcgttgagga agttgtcgcc aaagttttcg atgtcaatga gccactgagt
    12661 caaatcaatc aggcaaaact cgcctgacag aatttaatca agggcggtta gcgccctttt
    12721 catccctgtc tgaaatttct caaattctaa aaatctcaac caaacttatc tgataacact
    12781 aaattcgaaa gaatgcgtac aggtaagtaa caatgaaaaa aattgctatt gtgggtgccg
    12841 ggcctacggg gatctacacc ttattctcgc ttctacagca acaaactcca ctttctattt
    12901 ctatcttcga gcaggctgac gaggccggtg tcgggatgcc atacagtgat gaggaaaact
    12961 caaaaatgat gctggcaaat attgccagta ttgaaatacc gccgattaat tgtacgtatc
    13021 tcgaatggct acaaaagcaa gaagccagcc atctccagcg ttatggcgtt aaaaaagaaa
    13081 ccttgcacga tcgtcagttt ttaccgcgaa ttctgctggg cgaatatttc cgcgatcaat
    13141 ttttacgatt agtagaccag gcacgacagc aaaaatttgc agtggctgtt tatgaatcat
    13201 gccaggttac cgatctgcaa attacaaatg ctggcgtcat gctcgctaca aatcaggatt
    13261 tacccagcga gacgtttgat ttagcggtga ttgccacggg tcacgtctgg cctgatgaag
    13321 aagaagcaac cagaacgtat tttcccagtc cgtggtcagg cctgatggaa gcaaaggtcg
    13381 atgcgtgtaa cgtgggtatt atgggaacat ccttgagcgg actggatgcg gcaatggcag
    13441 tggctattca gcatggttcg ttcattgaag atgataaaca acacgtcgtt tttaaccgcg
    13501 ataacgcaag tgaaaagcta aatattacgt tgatgtcgcg cacgggtatt ttacccgaag
    13561 ccgatttcta ttgccctatt ccctacgagc ccttacacat cgtcaccgat caggcattaa
    13621 atgctgagat tcaaaaaggt gaagagggcc ttttggatcg ggtatttaga ttgatagtag
    13681 aggaaatcaa gtttgctgat ccagactgga gccaacgcat agccttagag agcctgaatg
    13741 tcgattcctt tgttcaagcc tggtttgccg agcgcaaaca acgcgaccca tttgactggg
    13801 cagaaaaaaa tctccaggaa gtcgaacgca ataaacgaga aaaacatact gttccctggc
    13861 gttatgtcat tctgcgcctg catgaagccg tacaggaaat tgttccacat ctgaatgaac
    13921 acgaccataa acggttcagt aaaggccttg cccgggtttt tatcgataat tatgcggcaa
    13981 tcccttcaga gtctattcgt cggctgctgg ccttacgtga agcggggatc attcatattc
    14041 tcgccctcgg tgaagactac gaaatggaaa ttaatgagtc gcgcaccgtc ctgaaaacgg
    14101 aagacaacag ctactcgttt gacgttttta ttgatgcccg cggacagcgt ccgcttaaag
    14161 tgaaagatat tcctttccct gggctacgcg agcaattaca gaaaacaggg gatgaaatcc
    14221 ctgatgttgg cgaagattat acgttacagc aacccgaaga tattcgtgga cgcgtagcgt
    14281 tcggcgcgtt gccctggttg atgcacgacc agcctttcgt tcagggactt acggcatgtg
    14341 cagaaattgg tgaggcgatg gctcgggcgg tcgtaaaacc tgcatcccgt gcacgtcggc
    14401 gtctttcgtt tgattaaagg taaagctatc agcactcaac aaactgatgc tgttcattaa
    14461 gacgatagcg gactccggtt cgaatattgt tttcaccttc aatggcgacg gcgaagcgaa
    14521 ctctttcgcc gtcatgatat gccagtgcgg cgcttccccc tgacccgaga ataattgagt
    14581 cgaccacgcc cgtgctcact accgttgaat tttcaccgct ggcaatgatg tgtacattgt
    14641 cgccgctgtt ggcaattctg gcgtttgcgc caaagctggc gatttgcacc aggtcaccat
    14701 tactggcgac atgacaccgt tcacccaatg tacaaacgcg tactcgcatt ccggtattcg
    14761 caatccgact actatctcca gcgctgctta tccgcgaaga attaccggtt acagcaaccc
    14821 gtgcgcgttc accggagctg ccaatgtgac tgttaaagcc gacacttcca atccgcacgc
    14881 tataacccat actggcaatt tgcgcggcat acccagcgat tgcgattcgg gcgttatcct
    14941 cacaacagcc gatgcgagca tgatcgtcgc tgcgggcaaa ctgactgcaa aatggtatct
    15001 ctccggggtc tgtggccagt ttttgcatag aggaaacttc ctgatgcgcg aagttttcat
    15061 ccgccagcca ttttttccac gcatactcaa ccagactctc cgcccagtcc gtatatcctt
    15121 cctcaatcag tgcatgatga atatcggcat aactaccgcc gcgtcggaaa tggcgcagga
    15181 accagcgata catcaccgct ccgatacgcc attctcgcag gtctgctcgt gtaatgatca
    15241 ttcgttagtg ccggtgatag ccatcgacca tgcttttaaa ggtttcgtgt ggcgtacgcc
    15301 ccgtggtgca aagataaaga tgtccgaaga taaaaaagag gcttataaat gccagagcaa
    15361 aatgcgcctg caataaccag tatcttacgc caggaaacac atctcccacg gcttgcggat
    15421 agagacacaa cagccccgtc aatagtaaca acggcagcaa tccatacatg acaccaacat
    15481 aggcgacctg ctgtaaggga ttaaatttag actgtgttgt tgccgggaaa ggatgttctt
    15541 ccccctgcat aatgccaaac aaataaaatc gcgtttgttt tgccgctcgt tccagccacc
    15601 cctgacgacg aatgcgatag tggtgaccat tatccccaac ggcattgatc agcacaaagc
    15661 cgagccagca tgccagtaac aaaaatccgc aaacttcatg caccgcaacc agacttttaa
    15721 ccgcagttgc gcccaccatc gcaaagtgat ttatcagccc acttgccagc aacaatacaa
    15781 agagtaacgc attcgaccag tgccataatc tgaccgcttt gctgtaaaga taaaccttct
    15841 caccatgatc ggtggctgat ttcttcacgc cacgagcacg aagcaaggcg tgcagcccca
    15901 gcaccaacca catcccaacc agcaatactc cggcaatgat caaccacacc ggccaaaatt
    15961 cgggagtgaa ctgtggcaca tagttcgcta actggctctg aaactgttca gcatgttgcg
    16021 acgggttcat acattttcct ttttaatcaa atgttgaccg aaccggcgat acagatgcgg
    16081 tttcccggca ccaggtagct gatattgata gtatttatta tcctgcaacc acgcctgaat
    16141 ttctgggcta tcttcgcgac caaagatcaa tgcgtgttcc ggacaggcgc tgacgcaaat
    16201 gggcggaaag ccttttgcca gtcgggactc agcgcagaaa tcacatttat ctgccacttt
    16261 agtgacaggg ttaaggtagc gaacctggta cggacaagcg ccaatgcaat aactgcagcc
    16321 gatgcactgc gatttttcta cccgcacgat cccctgctca tcacgccacg atgcccccgt
    16381 cgggcaaacg tcgatgcacg gagcatcttc gcagtgctga catgactggc gaaaaaagtg
    16441 atactgcgtt tcgttgtcgt tatcggtgac tggaatgtgt gctatcgata aacggcttcc
    16501 ctgagcgggg acgtggttag ttttacggca tgcgcgggcg caaatattac agccattgca
    16561 ccgtgactcg tcatgaatca tggcataccg aacttccttt tcttgcctcg tgttcgctaa
    16621 cagagaagac gcagaaccag taaaaaagat tactgttccc atccccagaa caaatttgcg
    16681 tcgagtgaag gacatctgat tatcccctaa tgcagtggct gcaactgtcc accctatcca
    16741 tgacatctcc ctgaaatttc aggggttctg aaactgctct acctcgaacc attcgcataa
    16801 gccataagcc ttgccatagg tcataaatcg ctcactaaac aagtacaact ccgccgtcga
    16861 tgactccacc tgccgtatat cggcatattc aggtgcaaca tccatcgcgg cgatcgcggc
    16921 ttcaatctgc gcttcctgga agtaatatgg cgcttgcatc agcatggcga ctttgtaagg
    16981 acgaggatat gtctggcact caaaacgaac ggcgtgtgca atggcgcgac aaatgtcctg
    17041 ctccacaact tgcaaagaca tcgccgcgta gttttcactc atggcctgcg ttgagtaata
    17101 gtaatcatcc tggcttcccg tcatacaagc gatatcagcg tagtctccgt tctgcttaat
    17161 gctttccagc aaaactgaca gctcctcttc tgcaaccgaa tagggtggcg agagaaacac
    17221 ggcgcgcgcc accagctgcc cagccgcaga atgctggcga atgaactctg ccaggatttc
    17281 agccggttct ggttcggtta ccgtatcaac ggggtgacgc atcccctgta accattgccg
    17341 cttcgcctcg tcaacttcgc tgactttcgc taacggcaat tcatcccgat ggttcatctc
    17401 acccattcct tacacaggca gtagattgtg tgctgccaga tcggcggcaa tatcttccag
    17461 ccccagacgc tgcaatgttt cgcgggtggg actaccaagc tgtgggtccc agcccatttc
    17521 tttgtagaac atcgtgagcg aagcatgcat atcgtcacga tccattttgt cagtaccttc
    17581 agtaaagacc gggatctgcg gatccttgtc gaatacccag gaacagataa gatcgtgttc
    17641 attacgcata tctttggttt gcataagttt taccgtgtag gcacgatgca acgtaaagat
    17701 acgctctgca gctaaatcta atttttcctg agtcatctct tcgccggtga tcgctttgaa
    17761 gaatttggct tcaagcgcca gatcgccccg gtaattacgg cttttcagcg gcgaaacggt
    17821 cattggccag acccagttac acagggtgac ggcgttatgc aaacagaccc gcaacagcga
    17881 ccacttagca tatttaattt ttgcgtcatt gattggcgtg tagtttttgg tttcatcgta
    17941 ggcatcttca gaaccaaaaa gctctttcgc cacttcacgt tgcagtttca atggcaagcc
    18001 ggaaccaata aagttgatat gggtatgtgt catgcagtca cggttgaaca tacagttaac
    18061 aatggaaccg acctgcgctg acgcttcatt ggcatggtga accggatagc caaacggcga
    18121 ccagagttta tttttcgcgt agccccagta ctcttcaccc aaattccagc gttctgcgat
    18181 ggcatatgaa ccatcagcca ggtgactcag ctcacccaca cgatgcgcca gacggtagta
    18241 aaaatcttta atgaaattaa cgtcacccgc ttccagttga tcccagtgaa tttccgcata
    18301 ctcttcagca ggcagaacac acttgaacac ccctttgctg taacaataag taaagtcgcg
    18361 atgcaactgc ccgtagttac accataggcc gtagtcatcg aacaaattca gaccaaccaa
    18421 gttaccaatc acacggccat cgtctttatc ttcaaaatct ttcggaccgt tcgggaagat
    18481 ggtggtatgg acaaagttgg ctacacaagt gttaccacct gtgctgggca cgccaaactc
    18541 tttcacccga ggaatattca tttgggtcat acaacggatc gggcaagagt ggcagccact
    18601 catttttact gtgtatttct ctgccgccgg acctaagtca aaaacggatt tataggtacg
    18661 aaagccgacc gtgttctgat tgcctggcgg aatttccccc gtttcaatcg gaccaccttc
    18721 agccgcgccc caaaacagcc ctttacgtgc tgtccaacgt gacttgggat ctgaatactc
    18781 tgcccacgat tgcggcgtac ttggcacgac atggttgtta ttcgcaccaa taagttcagt
    18841 catcatgtaa tcgttgagac gcttcatctc ctgacgatcg gcaatgttga cccctttcgt
    18901 cccttcaacc gcaatcgctt tcaggttttt cgaacccata attgcgccag ttcccgcacc
    18961 gccgctgtgg ttacggctat tcaacatgcc agagagagga acaaggtttt ccccagcctg
    19021 gccaatagcc gccacacagg tttccggact ggtcaatcga caaatttctt ccgtcgttgc
    19081 gcgcgtccct tttccccata agaaatcggc tttttccagg ctaactttgt catctttaat
    19141 cttcagccat accggtgatt tcgccttccc ttcgataata atgacgtcgt atccagcgaa
    19201 tttcatttgc gctgcaaaaa agccacccat atgggcatcg acaactaaat ttcctttggt
    19261 aaaagtagaa agtgaggtga tatttacgcg agaactacag ggggcaccag atccagttaa
    19321 tgggccggta gcaaagacta atttattcgc ttcatcgaaa ggtttcgtgc ctggcggtac
    19381 ttcgtcatac ataattttgt agccgaagcc catgccaccg acaaaacttt taaacttact
    19441 ggaatcttcg agggtaatat ttcctgtcgt gagattgact cttaatatat tacctgtcca
    19501 accgttagcc atgatttttt cctttgcaag attctcacag taatatcttt ccactcgata
    19561 atttttaacg cccctgttgg gcaggcgttt gcacattcac cgcataacac acatttcgag
    19621 gattttttac tttcggtatt tacggtggcc atcatccacg ggcatgccgt agtacaggcg
    19681 ctacagccaa tacagcgttt atgatcgacg gtaatacagc cttctttctg ctgccaggta
    19741 atcgcaccaa tcgggcagac gttcatgcat tgcggctctt tgcattgacg gcaggtgtcc
    19801 gcggtatagt tgagatcgcc atacaggccg ccgccagagc caaccccgtt gtcgccaaag
    19861 aaataattgc gatggatttt gatacgggag aagaatgtcc ctactgagcc atcgttgaag
    19921 ttggtacaag agatttcaca gcggtggcaa cccgtgcatc gcgctcgctg ggtcaccagc
    19981 acccctttgg gtgtatttat caaccctact gtgccgctat caatatcttc ctgtttgcaa
    20041 cccaaaagtg acagtaacgc aggggcaatt gttagaccag caaggccttt tcctgatata
    20101 cgcaagaatt ctaatcgcgt taagccaata tctaatagtg gacgatcaac cgggttcatt
    20161 tatttcgcat cctcttcgca aacacagtgc tgacaattca gcagcctgat attaagcatt
    20221 caattaatac gctgctgact ctgtccccaa caccagatat tttccctaac ccgatagtgt
    20281 atgagaaaag atttaacgcc gataaactaa tcctttatgg ttaggagaaa aaataatgtg
    20341 attattctga gcccttaaca ttgatcgtta tcaattaaaa ttacaaagca agaaattata
    20401 agtga
//
LOCUS       DAJVUJ010000066        20170 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.066, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000066 DAJVUJ010000000
VERSION     DAJVUJ010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20170
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.066"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            144..347
                     /locus_tag="OQQ48_002161"
     CDS             144..347
                     /locus_tag="OQQ48_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HCS1952926.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLLRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            355..1287
                     /gene="aaeA"
                     /locus_tag="OQQ48_002162"
                     /pseudo
     CDS             355..1287
                     /gene="aaeA"
                     /locus_tag="OQQ48_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
     gene            1293..3260
                     /gene="aaeB"
                     /locus_tag="OQQ48_002163"
     CDS             1293..3260
                     /gene="aaeB"
                     /locus_tag="OQQ48_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HCS1952927.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IIADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTGLQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQQRLLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            3352..3624
                     /locus_tag="OQQ48_002164"
     CDS             3352..3624
                     /locus_tag="OQQ48_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HCS1952928.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(3680..3943)
                     /gene="yhcN"
                     /locus_tag="OQQ48_002165"
     CDS             complement(3680..3943)
                     /gene="yhcN"
                     /locus_tag="OQQ48_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HCS1952929.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(4309..4779)
                     /gene="argR"
                     /locus_tag="OQQ48_002166"
     CDS             complement(4309..4779)
                     /gene="argR"
                     /locus_tag="OQQ48_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HCS1952930.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            5214..6152
                     /gene="mdh"
                     /locus_tag="OQQ48_002167"
     CDS             5214..6152
                     /gene="mdh"
                     /locus_tag="OQQ48_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCS1952931.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            complement(6215..7282)
                     /gene="degS"
                     /locus_tag="OQQ48_002168"
     CDS             complement(6215..7282)
                     /gene="degS"
                     /locus_tag="OQQ48_002168"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HCS1952932.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGVNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(7372..8739)
                     /gene="degQ"
                     /locus_tag="OQQ48_002169"
     CDS             complement(7372..8739)
                     /gene="degQ"
                     /locus_tag="OQQ48_002169"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HCS1952933.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDCGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            complement(8893..9291)
                     /locus_tag="OQQ48_002170"
     CDS             complement(8893..9291)
                     /locus_tag="OQQ48_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HCS1952934.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            9485..10612
                     /gene="zapE"
                     /locus_tag="OQQ48_002171"
     CDS             9485..10612
                     /gene="zapE"
                     /locus_tag="OQQ48_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HCS1952935.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLHDETRAQMDKLWLALAGAKRENSPTLEINHRPLATMGAENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            10830..11258
                     /gene="rplM"
                     /locus_tag="OQQ48_002172"
     CDS             10830..11258
                     /gene="rplM"
                     /locus_tag="OQQ48_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCS1952936.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKCTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            11274..11666
                     /gene="rpsI"
                     /locus_tag="OQQ48_002173"
     CDS             11274..11666
                     /gene="rpsI"
                     /locus_tag="OQQ48_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCS1952937.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            12061..12699
                     /gene="sspA"
                     /locus_tag="OQQ48_002174"
     CDS             12061..12699
                     /gene="sspA"
                     /locus_tag="OQQ48_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HCS1952938.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            12705..13202
                     /gene="sspB"
                     /locus_tag="OQQ48_002175"
     CDS             12705..13202
                     /gene="sspB"
                     /locus_tag="OQQ48_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HCS1952939.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(13245..14611)
                     /gene="dcuC"
                     /locus_tag="OQQ48_002176"
                     /pseudo
     CDS             complement(13245..14611)
                     /gene="dcuC"
                     /locus_tag="OQQ48_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
     gene            14991..15782
                     /gene="nanR"
                     /locus_tag="OQQ48_002177"
     CDS             14991..15782
                     /gene="nanR"
                     /locus_tag="OQQ48_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HCS1952940.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            15904..16797
                     /gene="nanA"
                     /locus_tag="OQQ48_002178"
     CDS             15904..16797
                     /gene="nanA"
                     /locus_tag="OQQ48_002178"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HCS1952941.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            16906..18396
                     /gene="nanT"
                     /locus_tag="OQQ48_002179"
     CDS             16906..18396
                     /gene="nanT"
                     /locus_tag="OQQ48_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HCS1952942.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVSGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            18444..19133
                     /locus_tag="OQQ48_002180"
     CDS             18444..19133
                     /locus_tag="OQQ48_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCS1952943.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            19130..20005
                     /gene="nanK"
                     /locus_tag="OQQ48_002181"
     CDS             19130..20005
                     /gene="nanK"
                     /locus_tag="OQQ48_002181"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HCS1952944.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPQALRDA
                     LAALVAPLQAHAQQVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLSNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELMGADARTIFTRAGQGDEQAQQLIHRSA
                     HVLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            20002..>20170
                     /locus_tag="OQQ48_002182"
     CDS             20002..>20170
                     /locus_tag="OQQ48_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCS1952945.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQS"
BASE COUNT         4837 a         5031 c         5258 g         5044 t
ORIGIN      
        1 tagataacag cataacagtt acgctaatat attaaatatc aatctacagc aatgttgctc
       61 tcgcccggct ttccatgccc ttctctgcgg cgacagatgc tgaaaataat aacgcctgct
      121 ctctctatac aaccaaggtc aacatgagtc tgtttcccgt tatcgtggtg tttgggctgt
      181 ccttcccacc gatatttttt gaattgcttt tatcactggc gattttctgg ctgctgcgcc
      241 gggtacttgt gccaacaggt atctacgact ttgtctggca tccggcgttg ttcaacaccg
      301 cgctctattg ctgcttgttt tatttgatat cgcgactgtt cgtttgaggt tgaagtgaaa
      361 acactaataa gaaaattctc ccgtacggcc atcacggtcg tattagtcat tctggccttc
      421 atcgcaattt ttaatgcctg ggtctattac accgaatccc cctggacgcg tgacgcgcgc
      481 tttagcgctg acgtcattgc gatcgcgccg gacgtttctg gactcattac ccaggtgaat
      541 gttcatgata accagctggt gaaaaaagga cagatactgt tcaccatcga ccagccgcgc
      601 tatcaaaagg cgcttgagga agcgcaagcc gatgttgctt attatcaggt actggcacag
      661 gagaaacgcc aggaggccgg acgtcgtaac cgtctcggtg tgcaggcgat gtctcgcgaa
      721 gagatcgact aggctaacaa cgtactacaa acggttctgc atcagttagc gaaagcgcag
      781 gcgacccgcg atctggcaaa actggatctt gaacgcacgg tgatccgcgc gccagcagat
      841 ggctgggtga ccaacctcaa cgtctatacc ggtgagttta ttactcgagg atcaacggcg
      901 gttgcgctgg tgaaacagaa ctccttctat gtactggcct atatggaaga aactaagctg
      961 gaaggggtgc gtccggggta tcgtgcagag atcacgccgc ttggcagtaa caaagtgctg
     1021 aaagggactg ttgatagtgt tgccgcaggg gtcaccaacg ccagcagcac gcgtgacgac
     1081 aaagggatgg cgactataga ctctaacctt gaatgggtgc gtcttgcgca acgtgttccg
     1141 gttcgtattc gtctcgacaa ccagcaagag aacatctggc ctgcgggcac cactgctaca
     1201 gtggtggtca ctggcaaaca agatcgcgac gaaagccagg attcgttctt ccgtaaaatg
     1261 gcccatcgcc tgcgtgagtt tggttaatca cgatgggtat tttctccatt gctaaccaac
     1321 atattcgctt tgcggtaaaa ctggcgaccg ccattgtact ggcgctgttt gttggctttc
     1381 acttccagct ggaaacgcca cgctgggcgg tactgacagc ggcgattgtt gccgccggtc
     1441 cggcctttgc tgcgggaggt gaaccgtatt ctggcgctat tcgttatcgt ggctttttgc
     1501 gcatcatcgg cacatttatt ggctgtattg ccggactggt gatcatcatt gcgatgatcc
     1561 gcgcaccatt attgatgatt ctggtgtgct gtatctgggc cggtttttgt acctggatat
     1621 cctcgctggt acgaatagaa aactcgtacg cgtgggggct ggccggttat accgcgctga
     1681 tcattgtgat caccattcag ccggaaccat tgcttacgcc gcagtttgcc gtcgaacgtt
     1741 gtagcgagat cgttatcggt attgtgtgtg cgattatagc ggatttgctc ttttctccgc
     1801 gatcgatcaa acaagaagtg gatcgagagc tggaaagttt gctggtcgcg caatatcaat
     1861 taatgcaact ctgtatcaag catggcgatg gtgaagttgt cgataaagcc tggggcgacc
     1921 tggtgcgacg caccacgggg ctacaaggca tgcgcagcaa cctgaatatg gaatcttccc
     1981 gctgggcgcg ggccaatcga cgtttaaaag cgatcaatac gctatcgctg acgctgatta
     2041 cccaatcctg cgaaacttat cttattcaga atacgcgccc ggaattgatc actgatactt
     2101 tccgcgaatt ttttgacacg ccggtagaaa ccgcgcagga cgtccacaag cagctcaaac
     2161 gcctgcggag agttatcgcc tggaccgggg aacgggaaac gcctgtcacc atttatagct
     2221 gggtcgcggc ggcaacgcgt tatcagcttc tcaagcgcgg cgttatcagt aacacaaaaa
     2281 tcaacgccac cgaagaagag atcctgcaag gcgaaccgga agtcaaagta gagtcagccg
     2341 aacgtcatca tgcgatggtt aacttctggc gaaccacact ttcctgcatt ctgggcacgc
     2401 ttttctggct gtggacgggc tggacttccg gcagtggtgc aatggtgatg attgcggtag
     2461 tgacgtcact ggcaatgcgt ttgccgaatc cacgcatggt ggcgatcgac tttatctacg
     2521 ggacgctggc cgcgctgccg ttagggctgc tctacttttt ggtgattatc cctaataccc
     2581 aacagagcat gttgctgctg tgtattagcc tggcagtgct gggatttttc ctcggtatag
     2641 aagtacagca acggctactg ggctcgatgg gggcgctggc cagcaccata aatattatcg
     2701 tgctggataa cccgatgact ttccatttca gtcagtttct cgacagcgca ttagggcaaa
     2761 tcgtcggctg tgtgctcgcg ttcaccgtta ttttgcttgt gcgggataaa tcgcgcgaca
     2821 ggaccggacg tgtactgctt aatcagtttg tttctgccgc tgtttccgcg atgactacca
     2881 atgtggcacg tcgtaaagag aaccacctcc cggcacttta tcagcagctg tttttgctga
     2941 tgaataagtt cccaggggat ttgccgaaat ttcgcctggc gctgacgatg attatcgcgc
     3001 accagcgcct gcgtgatgcg ccgatcccgg ttaacgagga tttatcggcg tttcaccgac
     3061 aaatgcgccg cacagcagac catgtgatat ctgcccgtag cgatgataaa cgtcgtcggt
     3121 actttggcca gttgctggaa gaacttgaaa tctaccagga aaagctacgc atctggcaag
     3181 cgccaccgca ggtgacggaa ccggttcatc ggctggcggg tatgctccat aagtatcaac
     3241 atgcgttgac cgatagttag gtcaaaacca acgccaaaag tgtcggtttt ttcatggcta
     3301 tacttagcga tgcacggcag aactcgccgc gaaacgtgac ggtggcaata gatgaatatt
     3361 tatacctttg attttgatga gattgagagt caggaggatt tttatcgtga ctttagccaa
     3421 acctttggtc tggcgaaaga taaggtacgc gatctcgact cactatggga tgtgttaatg
     3481 aacgatgtcc tgccgctacc acttgagatt gaatttgttc atctgggaga gaaaacgcgt
     3541 cgccgttttg gcgcgttaat attgctgttt gatgaggcag aggaagagct ggaagggcat
     3601 ttgcgtttta atgttcgtca ttagcgcaaa aaaaagcccc cgaaccgggg gcaatatcgt
     3661 cggacaagac gatgagggtt tatttgtaca gttcagccgt agcgtgccag gtgtcaccgc
     3721 tacgagcttc agtaatctgg taggccgttg cgcctttctc ttccgctttt ttgttcagca
     3781 tttcacgcat atccattggc gaagacgcca caccacttac ggatacggtc ccgattgctt
     3841 cacgattttg tgcttgtgca gcatcaatgg agtcggcagc gaatgcaccg aaagagagaa
     3901 cagaaagtac gcttaatgca gctacagtgg ttttgatttt catgattttt acctcgacat
     3961 aatcttttag ctgggccttt gttttgtgac ccatatcaca aaatcaagca tacactaatt
     4021 attgaactaa ttaataccac gctaactgtt tcagttgata ctaagtgtta aaaatgtgaa
     4081 ttttaataac gaaaaagaat taaaaacacg gtcatttatt tttaattcta gtattttcaa
     4141 ttagataatg aattttgcat aatgtgcttt ttttaccgta tgtattcatt atgtgaatga
     4201 catgtcgcag taaaactgca ctattcgtta aattaatcgc aggggaaagc agggggttga
     4261 gagggataag caacattttc cccgccgcca gaagcgacgg ggcagagatt aaagctcctg
     4321 gtcgaacagc tctaaaatcg cttcgtacag gtctttgacg gtgaaaccgt tagcaggggt
     4381 ggtaaagatg gtgtcatcgc cagcgatggt gcccagaata ccttctgctt tgcccagtga
     4441 gtccagcagg cgagcaatta actgcgccgc gccagggctg gtatgaatca cgacaactgc
     4501 atcgttgtag tcgatatcca gcaccagatt cttcaatgga ctggaggtgg ttggtacacc
     4561 cagttcagct ggcaggcagt aaaccatttc cattttggca ttgcgtgtac gtacagcacc
     4621 aaacttggtc aacatccgcg agactttaga ctgattaata ttgtcaaagc cttgctcctg
     4681 caacgcggcg acgatttcgc cctgggagct aaatttctct tctttaagta atgctttaaa
     4741 tgctttaact agttcttctt gcttagccga gcttcgcata agtcacccga tatggtggtt
     4801 gatacaacat tattgtgcat acagatgaat ttttatgcaa acagtcaacc ctgaagaagg
     4861 ctgaaataat gttatgaaag agcgggattt tatcaaattt cgttattgag aaacatgcct
     4921 gcgtcacggc atgcaaattc tgcttaaaag taaattaatt gttatcaaat tgatgttgtt
     4981 ttggctgaac ggtagggtat attgtcacca cctgttggaa tgttgcgcta atgcataagc
     5041 gactgttaat tacgtaagtt aggttcctga ttacggcaat taaatgcata aacgctaaac
     5101 ttgcgtgact acacattctt gatatgtggt cattgtaaac ggcaattttg tggattaagg
     5161 tcgcggcagc ggagcaacat atcttagttt atcaatataa taaggagttt aggatgaaag
     5221 tcgcagtcct cggcgctgct ggcggtattg gccaggcgct tgcactactg ttaaaaaccc
     5281 aactgccttc aggttcagaa ctctctctgt atgatatcgc tccagtgact cccggtgtgg
     5341 ctgtcgatct gagccatatc cctactgctg tgaaaatcaa aggtttttct ggtgaagatg
     5401 cgactccggc gctggaaggc gcagatgtcg ttcttatctc tgcaggtgta gcgcgtaaac
     5461 cgggtatgga tcgttccgac ctgtttaacg ttaacgccgg catcgtgaaa aacctggtac
     5521 agcaagttgc gaaaacctgc ccgaaagcgt gcattggtat tatcactaac ccggttaaca
     5581 ccacagttgc aattgctgct gaagtgctga aaaaagccgg tgtttatgac aaaaacaaac
     5641 tgttcggcgt taccacgctg gatatcattc gttccaacac ctttgttgcg gaactgaaag
     5701 gcaaacagcc aggcgaagtt gaagtgccgg ttattggcgg tcactctggt gttaccattc
     5761 tgccgctgct gtcacaggtt cctggcgtta gttttaccga gcaggaagtg gctgatctga
     5821 ccaaacgtat ccagaacgcg ggtactgagg tggttgaagc gaaagccggt ggcgggtctg
     5881 caaccctgtc tatgggccag gcagctgcac gttttggtct gtctctggtt cgtgcactgc
     5941 agggcgaaca aggcgttgtc gaatgtgcct acgttgaagg cgacggtcag tacgcccgtt
     6001 tcttctctca accgctgctg ctgggtaaaa acggcgtgga agagcgtaaa tctatcggta
     6061 ccctgagcgc atttgaacag aacgcgctgg aaggtatgct ggatacgctc aagaaagata
     6121 tcgccctggg cgaagagttc gttaataagt aattgattag cgaataataa aaaaccggag
     6181 cacagactcc ggttttttgt tttgagcact cgacttaatt ggttgccgga tattcctgaa
     6241 tggtgacctg cagcgttaac tgcttatcat cacgcatcac tacaaccggg atcaccgaac
     6301 cagggcgaat ttctgccacc tgatccatcg tctccagagc agagatggcc ggtttgttat
     6361 ccaccgaaat aatcagatcg ttgacctgaa tacccgcatt cgccgccggg ccgtcaggtg
     6421 acacttcatt aaccacgatc ccttgcagtt gatctatacc accgctctgc gcgtgcagtg
     6481 gtgcgatctc acgtccacca atgccaatgt agccgcggat cacgcgccca tcgcggatca
     6541 gcttatccat aattttggtt gctaactgga aaggaatcgc aaagccgata ccttccggcg
     6601 tttcgccatc gttactctta tcaaacgaca gcgtgttaat gcccatcagt tctcccagcg
     6661 agttcaccag cgcgccgcca gagttaccgt ggttaatgga agcatcggtt tgtaggaagt
     6721 tttgccgccc ggtagggttc agaccgattc gccccgtggc actaataatc ccttgggtaa
     6781 tggtctgccc gaggttgtac gggttaccga tcgccagtac tacgtcgcca atgtgcggta
     6841 cgcgacgtgc attaattgga atggtaggta aaccgccagt ggcattaatt ttaagtaccg
     6901 ccagatcggt tagagagtca gatcccacca gcaatgcttc aaatacacgt ccatcctgta
     6961 aggcgacgat gatctgatcg gcgtcgttga tgacgtgttt attggtgatg atataaccgc
     7021 gttgatccat gattacaccg gatcccaggg tgcggatctc aagctggttg tgagagttgg
     7081 tgttcacacc acggttgtaa acgttaacca ccgctggcgc ggcgcggcga accgccagat
     7141 tatagctggc aggcgtctca tcggtactgt caaattgcgg agtggaaagc gggttaaggc
     7201 tgcgcagcga aggcatggca accagcagaa tagcgccgac aattaatcca atcgcaacgg
     7261 aacgtaagag cttcacaaac atgatggagg cgtcattaaa aaagggaacg gcagcagcat
     7321 accacgagtt aaccggacat cacacgtaag cctgatgccc ggtttacgac attaacgcat
     7381 cagcagatag atgctttcat tgccgcgtac aatttgcagg gcgatgatgg ccggttttgc
     7441 cgccagcact ttacgcattt cagcaatcga gttcacccga tcgcggttga cgccaatgat
     7501 cacatcgtct ttttgcaagc ctgcctgagc agctgggctt cctttgacaa cttcatcgat
     7561 cttaatacct ttgccgcaat cttttagctg accatcgctc aacgttgcac cttccagcgc
     7621 tggcgtgatc atttcagcgc tggccgacga agaggtgctg gtatcaagcg tcacttctac
     7681 ttccagtggt ttgccgttac gcagcaggcc aagcttcact ttcgtgcccg gctctgtagt
     7741 cgcgatacga gagcgcaact cagcaaagct attcagcggt ttgccgttga ggctggtaat
     7801 aatatcgccc gctttgacgc ccgcttttgc cgagccagaa cctggcaaca cttcgctgac
     7861 aaacgcgcca cgctgcacgt caaggttgaa ggctttggcg atatcggcac tcatctcggt
     7921 gcctttgatg cctaacaaac cgcgtttgat ttcaccaaag tcgataagct gctgcgccag
     7981 tgttcgcgcc atattactgg ggatggcaaa tccaatcccg acgctcccgc cgccaggcgc
     8041 aaggattgca gtgttgatgc caattaactc accgttaagg tttaatagtg caccgccgga
     8101 gttaccgcgg ttaatggaag catctgtctg gataaagttt tccagacctt caagattcaa
     8161 cccgctgcgg cctaatgcgg aaacaatgcc agaggtggcg gtttgcccaa ggccaaatgg
     8221 gttaccgacc gctacggcaa aatcaccgac gcgcaattta tcggagtcgg caatagcgat
     8281 ttgcgttaat ttgctcgggt tttgaatttg taacagggcg atatcgctct ggtcatcgct
     8341 accaatcagt tttgcatcaa actcgcgccc atcattgagc tgaatactga ttttctgtgc
     8401 ctgattaatc acatggttgt tggtcagcac atagccttta ctggcgttga tgatgacacc
     8461 ggaacctaaa ccttcgaagg gttgtgcagg ttgatccggt aaatcatcac caaaaaactt
     8521 tttgaattct tccgggattt tctgtccctg actggccgtt ccttccaccc gtacgctcac
     8581 cactgccgga agcacttttt ccagcattgg agccagactg gggagagggg cctgatcggc
     8641 aacctggcct ggaatcgacg cgacggcctg aaatgacgcc gagagagtta acccgacact
     8701 taacgctaat gcactcaaca gctgggtttg ttttttcatt attcctgctc tcgtacctga
     8761 atgataagaa aagagattca aaacagtttg atgttattga attttcagac ggctaacaat
     8821 gagacaccag attaaatgat agtcggggag ggagagaaga ggagggcgca atggctgcgc
     8881 ccgaaaaata aattagtcgc gcttcgcgcc agtacgcagc aggccggatg caccttcaga
     8941 atagtcgcga ggcatctgca ccggtgcctg atcgttgctg gcttcagact ctgccagacg
     9001 attacggaac gggtttgctt cagcagacag ttccggcagc aggctgctgg agcttttcgc
     9061 catgtgctga tacagctggc gatagtcgtg cgccatggta tccagtaatt ccgcgctgcg
     9121 ggcaaagtgg ctaaccagct cttcgcgata ctcgtccagt tcagctttat tcttttccag
     9181 ttcgtactgc aacgcctgtt gctggcgtag tttacgatta ccaaaacgca tggccacagc
     9241 accaataatg atgccgacga ctaacccaat tagcgcatat tcccaggtca tgaacatctc
     9301 ccgttgtctt ttgtttccgt agggtgttgg cttcaggctc catgcctacg gctgattatg
     9361 ccactataac cgttaattcc acagaagtgg aatcccgact gcatatcgcg tagtgtagaa
     9421 cggccttttt ttcgtcaacc gtgaacaacg gcgcaccgat tattcaagga ataataataa
     9481 gatcatgcaa agcgttaccc caacatcgca atacctgaag gcgctcaatg aaggcagcca
     9541 tcaacccgac gacgttcaaa aagaggccgt cagccgcctg gaaattattt atcaagaact
     9601 catcaatagc aggccaccag cccccaggac gagtgggcta atggcgcggg tcggtaagct
     9661 gtggggtaaa cgcgaagaca caaagcatac gccagtgcgt ggcttatata tgtggggcgg
     9721 tgtaggacgc gggaaaacct ggctgatgga ccttttctat caaagcctgc cgggagagcg
     9781 gaaacagcgc ctgcactttc accgttttat gctgcgggtg cacgaagagc taactgcctt
     9841 acaggggcag accgatccgc tggaaattat tgccgatcgc tttaaagccg aaactgacgt
     9901 gctctgtttt gacgaatttt ttgtttctga tattaccgac gccatgctac ttggcggtct
     9961 gatgaaagcc ctgttcgccc gcggtattac cctggtagca acgtcaaata ttccgccgga
    10021 cgaactttat cgaaatggct tgcaacgtgc gcgttttctg cctgcaatcg atgccattaa
    10081 acagcattgt gatgtaatga acgtggacgc tggtgttgat tatcgactgc gtacactcac
    10141 tcaggcgcat ctgtggcttt cgccactcca cgatgaaacc cgggcacaaa tggataaact
    10201 atggttggcg ctggcgggag cgaaacgcga aaattcaccg acgttagaaa tcaaccatcg
    10261 accgctggcg acaatgggcg ctgagaatca gacgcttgct gtctctttta ccaccttgtg
    10321 tgtcgatgcc cgcagccagc atgactatat tgcgctctca cgcctctttc atacggtcat
    10381 gttgtttgat gtaccagtta tgacgcggtt gatggagagc gaagcgcggc gctttattgc
    10441 gctggtggat gagttttacg agcgccatgt caaattagtg gtgagtgcag aagtgccgct
    10501 gtatgaaatt tatcagggcg atcggctgaa gtttgagttc cagcgttgcc tgtcacgtct
    10561 gcaagagatg caaagcgaag agtatctgaa gcgcgagcat ttggcgggtt aaaacctgtc
    10621 acaaatcaca aaaaggggtc gatctttgac cccgacttct ctataatcct gcgaccccac
    10681 gttacaagaa agttttttcc caaaactttt tgtgtgctgg cataggctat tcgaaggggt
    10741 aggtttgccg gactttgtcg tgtgaacctc aacaattgaa gacgtttggg tgttcaccaa
    10801 cgtgtaacta tttattgggt aagcttttaa tgaaaacttt tacagctaaa ccagaaaccg
    10861 taaaacgcga ctggtatgtt gttgacgcga ccggtaaaac tctgggccgt ctggctactg
    10921 aactggctcg tcgcctgcgc ggtaagcaca aagcggaata cactccgcac gtagataccg
    10981 gtgattacat catcgttctg aacgctgaca aagttgctgt aaccggcaac aagtgtactg
    11041 acaaagtgta ctatcaccac accggccaca tcggtggtat caaacaagcg acctttgaag
    11101 agatgattgc tcgccgtcct gagcgtgtga ttgaaatcgc ggttaaaggc atgttgccaa
    11161 aaggcccgct gggtcgtgct atgttccgta aactgaaagt ttacgcgggt aacgagcaca
    11221 accacgcggc acagcaaccg caagttcttg acatctaatc gggattatag gcaatggctg
    11281 aaaatcaata ctacggcact ggtcgccgca aaagttccgc agctcgcgtt ttcatcaaac
    11341 cgggcaacgg taaaatcgta atcaaccaac gttctctgga acagtacttc ggtcgtgaaa
    11401 ctgcccgcat ggtagttcgt cagccgctgg aactggtcga catggttgag aaactggacc
    11461 tgtacatcac cgttaaaggt ggtggtatct ctggtcaggc tggtgcgatc cgtcacggta
    11521 tcacccgcgc tctgatggaa tacgacgagt ccctgcgttc tgaactgcgt aaagctggct
    11581 tcgttactcg tgacgctcgt caggttgaac gtaagaaagt cggtctgcgt aaagcacgtc
    11641 gtcgtccgca gttctccaaa cgttaattgg cttctgctcc ggcagaaaac aattttcgaa
    11701 aaaacccgct tcggcgggtt tttttatagc taaaatctga atcagcgtaa aaactggaaa
    11761 gttgcttttt gctgccacct gacagacagg taaaacaaaa accacatcgc caataaggga
    11821 ctaagtcaac tatttcagac taaagcgcat ctctttttcc ccatttccgg catcgactca
    11881 ccacaaaggt cgcaaaatct ggtaaactat catccaattt tctgcccaaa tgtcgggtat
    11941 tgctcatttt ttgtttgatt ttcgaacaaa gagagtcgtt ccttactggg taacacaact
    12001 tctgactggc cacctggtgg ctggtagcag taaaaattct gactatacct ggaggttttc
    12061 atggctgtcg ctgccaacaa acgttcggta atgacgctgt tttccggtcc tactgacatc
    12121 tatagccatc aggtccgcat tgtgctggct gagaaaggtg taagtttcga gatcgaacac
    12181 gtggaaaagg acaatccgcc tcaggatctg attgacctca acccgaatca gagcgttccg
    12241 accctggtgg atcgtgagct gaccctgtgg gaatctcgca tcattatgga atatctggat
    12301 gagcgtttcc cgcatccgcc actgatgcct gtttacccgg tagctcgcgg tgaaagccgt
    12361 ctgtacatgc atcgcatcga aaaagactgg tacacgctga tgaacaccat catcaacggt
    12421 tcagcttctg aagcagatgc cgcacgtaag caactgcgcg aagaactgct ggcgattgcg
    12481 ccggtcttcg gtcagaagcc gtacttcctg agcgatgagt tcagcctggt cgattgctat
    12541 cttgctccgc tgctgtggcg tctgccgcaa ctgggcatcg agttcagcgg cccgggtgcg
    12601 aaagagctga aaggctatat gacccgcgtc tttgagcgtg actctttcct tgcttcttta
    12661 actgaagcag aacgtgaaat gcgtctgggc cggagttaat ctgtatggat ttgtcacagc
    12721 taacaccacg tcgtccctat ctgctgcgtg cattctatga gtggttgctg gataaccagc
    12781 tcacgccgca cctggtggtg gatgtgacgc tccctggcgt gcaggttcct atggaatatg
    12841 cgcgtgacgg gcaaatcgta ctcaacattg cgccgcgtgc tgtcggcaat ctggaactgg
    12901 cgaatgatga ggtgcgcttt aacgcgcgct ttggcggcat tccgcgtcag gtttctgtgc
    12961 cgctggctgc cgtgctggct atctacgccc gtgaaaatgg cgcaggcaca atgtttgagc
    13021 ctgaagctgc ctacgatgaa gataccagca tcatgaatga tgaagaggca tcggcagaca
    13081 acgaaaccgt tatgtcggtt attgatggcg acaagccaga tcacgatgat gacactcatc
    13141 ctgacgatga acctccgcag ccaccacgcg gtggtcgacc ggcattacgc gttgtgaagt
    13201 aatacaaaaa caggccctgg tggcctgttt tgtcttttaa tggcttaata aaatagcgtg
    13261 attgtggcaa tcatattcac cacgaaaccg actgccatgg ggatcgctgt tcgcttcacc
    13321 accgcaaaag gcgaaacgcc tgcaattcct gacgtaacga ccaccaccgc agtaatcggc
    13381 gaaaccgcgc gcgctagcgt cgtggcaaaa tgcatcggca taatcattac aaccgctggt
    13441 acatgtagtc cagctgcgat attcggaata agactggcaa atgacataaa cggcgcattg
    13501 ccagagccca tcacaatggc acaaatggca atgaccagcg ccataataat catcacgcca
    13561 ataccgccca gaccagaatg ctccgcaccc ctgataaccg catcgacagt gccaatcgtc
    13621 gttaagcctt tcgcaaatat ttcacccgcg accaccagcg ttaccacgtt ggcaaactgc
    13681 gtacccatgc cgtcaaaaaa cgcctgcaca tcgtccattg tttcgcgcaa gttatgcttg
    13741 cggaagaact caacaaacat cgtcaccgtc aaactcagta acatcaccac caccagatgc
    13801 agttctgact gcatcagccc gacgtgggcg aggaacagcg agccgagcat caggattaac
    13861 ggcatcacag gtaaaatggc gtaatagagc ggcgggacat tatcgagagc tttttgctct
    13921 gcttgttcgt gattgatatt tttatctttt ttgtcaaaag cgcgttgcac gaaaaagtgg
    13981 gagatcgcca ccgagataat gacgcaagag gcgaccggaa gctggtagtg gaagaagtaa
    14041 gtggcaattt tcattcccgc gacctgggct gcaaaaatgg agttcgtttc cagaattccc
    14101 cactcaatgg acattgtggt tgcgataacc gccaccgcag agagacgact tactcccaga
    14161 ctcaccagtg tcggaaataa ggtgaccatc agcaacatac ccagaccgga ggcgctggta
    14221 ataaactgcg ccatgatttg accgatgacg taagttgccg acagaataat atacggtgag
    14281 cgaatgagtt ttaacgggcg gcttaacaag ctcaccatcg cgcgactggc ccctatgcgc
    14341 tccatgtagc gggcataccg cccaccgcca taatcgatag ccccaatccg gcaatgcggt
    14401 tggacagcat gcgcaggatt tcattataaa tatcgacgat caagtagccg ctgcttttgg
    14461 cgggctcgag tacaccaccg aaccctaacc agacaccgca catcatcagg aagatacctg
    14521 cggcagccag caccacctga ggtttgtagt ttttcaggat caaatagccc atcgtaatta
    14581 atacgatgac agatataatt atgccgaaca ttgttgaact ccgtgtcaaa agaaaacggt
    14641 caatcccata aacggcagat tgaaaacaat gatgttatat tttttgcaag tctatttatg
    14701 gtgcggatgt cgtgttttta attgtaggtg agataatttt tcattaaaaa atatgcgctt
    14761 atgattattt tgcaagaacg cattcataat attcataatg ctcgtgaata gtcttataaa
    14821 taattcaaac gggatgtttt tatctgcgtt acattaattt ttcgcaatag ttaattattc
    14881 cgttaattat ggtaatgatg aggcacaaag agaaaaacct gccattttcc cctactttca
    14941 atcctgtgat aggatgtcac tgatgatgtt aatcacactg accttacaga atgggcctta
    15001 tgaacgcatt tgattcgcaa accgaagatt cttcacctgc aattggtcgc aacttgcgta
    15061 gccgcccgct ggcgcgtaaa aaactctccg aaatggtgga agaagagctg gaacagatga
    15121 tccgccgtcg tgaatttggc gaaggtgaac aattaccgtc tgaacgcgaa ctgatggcgt
    15181 tctttaacgt cgggcgtcct tcggtgcgtg aagcgctggc agcgttaaaa cgcaaaggtc
    15241 tggtgcaaat aaacaacggc gaacgcgctc gcgtctcgcg tccttctgcg gacactatca
    15301 tcggtgagct ttccggcatg gcgaaagatt tcctttctca tcccggtggg attgcccatt
    15361 tcgaacaatt acgtctgttc tttgaatcca gtctggtgcg ctatgcggct gaacatgcca
    15421 ccgatgagca aatcgatttg ctggcaaaag cactggaaat caacagtcag tcgctggata
    15481 acaacgcggc attcattcgt tcagacgttg atttccaccg cgtgctggcg gagatccccg
    15541 gtaacccaat cttcatggcg atccacgttg ccctgctcga ctggcttatt gccgcacgcc
    15601 caacggttac cgatcaggca ctgcacgaac ataacaacgt tagttatcaa cagcatattg
    15661 cgatcgttga tgcgatccgc cgtcatgatc ctgacgaagc cgatcgtgcg ttgcaatcgc
    15721 atctcaacag cgtctctgct acctggcacg ctttcggtca gaccaccaac aaaaagaaat
    15781 aatgccactt tagtgaagca gatcgcatta taagctttct gtatggggtg ttgcttaatt
    15841 gatctggtat aacaggtata aaggtatatc gtttatcaga caagcatcac ttcagaggta
    15901 tttatggcaa cgaatttacg tggcgtaatg gctgcactcc tgactccttt tgaccaacaa
    15961 caagcactgg ataaagcgag tctgcgtcgc ctggttcagt tcaatattca gcagggcatc
    16021 gacggtttat acgtgggtgg ttcgaccggc gaggcctttg tacaaagcct ttccgagcgt
    16081 gaacaggtac tggaaatcgt cgccgaagag gcgaaaggta agattaaact catcgcccac
    16141 gtcggttgcg tcagcaccgc cgaaagccaa caacttgcgg catcggctaa acgttatggc
    16201 ttcgatgccg tctccgccgt cacgccgttc tactatcctt tcagctttga agaacactgc
    16261 gatcactatc gggcaattat tgattcggcg gatggtttgc cgatggtggt gtacaacatt
    16321 ccagccctga gtggggtaaa actgaccctg gatcagatca acacacttgt tacattgcct
    16381 ggcgtaggtg cgctgaaaca gacctctggc gatctctatc agatggagca gatccgtcgt
    16441 gaacatcctg atcttgtgct ctataacggt tacgacgaaa tcttcgcctc tggtctgctg
    16501 gcgggcgctg atggtggtat cggtagtacc tacaacatca tgggctggcg ctatcagggg
    16561 atcgttaagg cgctgaaaga aggcgatatc cagaccgcgc agaaactgca aactgaatgc
    16621 aataaagtca ttgatttact gatcaaaacg ggcgtattcc gcggcctgaa aactgtcctc
    16681 cattatatgg atgtcgtttc tgtgccgctg tgccgcaaac cgtttggacc ggtagatgaa
    16741 aaatatctgc cagaactgaa ggcgctggcc cagcagttga tgcaagagcg cgggtgagtt
    16801 gtttcccctc gctcgcccct accgggtgag gggaaataaa cgcatctgta ccctacaatt
    16861 tttataccaa agcgtgtggg catcgcccac cgcgggagac tcacaatgag tactacaacc
    16921 cagaatatcc cgtggtatcg ccatctcaac cgtgcacaat ggcgcgcatt ttccgctgcc
    16981 tggttgggat atctgcttga cggttttgat ttcgttttaa tcgccctggt actcaccgaa
    17041 gtacagggtg aattcgggct gacgacggtg caggcggcaa gtctgatctc cgcagccttt
    17101 atctctcgct ggttcggcgg cctgatgctc ggcgctatgg gtgaccgcta cgggcgtcgt
    17161 ctggcaatgg tcaccagcat cgttctcttc tcggccggga cgctggcctg cggctttgcg
    17221 ccaggctaca tcaccatgtt tatcgcgcgt ctggtcatcg gcatggggat ggcgggtgaa
    17281 tacggttcca gcgccaccta tgtcattgaa agctggccaa aacatctgcg taacaaagcc
    17341 agtggttttc tgatttcagg cttctctgtg ggggccgtcg ttgccgctca ggtctatagc
    17401 ctggtggttc cggtctgggg ctggcgtgcg ctgttcttta tcggcatttt gcctatcatc
    17461 tttgctctct ggctgcgtaa aaacatcccg gaagcggaag actggaaaga gaagcatgca
    17521 ggtaaagcac cagtacgcac gatggtagat attctctacc gtggtgaaca tcgcattgcc
    17581 aatatcgtaa tgacactggc ggcggctact gcgctgtggt tttgcttcgc cggtaacctg
    17641 caaaatgccg cgatcgtcgc tgttcttggg ctgttatgcg ccgcaatctt tatcagcttt
    17701 atggtgcaaa gtacgggtaa acgctggcca acgggcgtaa tgctgatggt ggtcgtgttg
    17761 tttgctttcc tctactcatg gccgattcag gcgctgctgc caacgtatct gaaaaccgat
    17821 ctggcttata acccgcatac tgtcgccaat gtgctgttct ttagcggctt tggggctgcg
    17881 gtggggtgct gtgtcagcgg cttcctcggt gactggctgg gaacccgcaa agcctacgtt
    17941 tgtagcctgt tggcctcgca gctgttgatt attccggtat ttgcgattgg cggcgcaaac
    18001 gtctgggtgc tcggtctgtt actgttcttc cagcaaatgc ttggacaagg gatcgccggg
    18061 atcttaccga aactgattgg cggttatttc gataccgacc agcgtgcagc gggcctgggc
    18121 tttacctaca acgttggcgc attgggcggc gcactggccc caatcatcgg cgcgttgatc
    18181 gctcaacgtc tggatctggg tactgcgctg gcatcgctat cgttcagtct gacatttgtg
    18241 gtgatcctgc tgattggtct ggatatgcct tcccgcgttc agcgttggct gcgcccggaa
    18301 gcattgcgta ctcatgacgc tatcgacggc aaaccattta gcggtgccgt gccatttggc
    18361 agcgccaaaa acgatttagt caaaaccaaa agttaatcct gttgcccggt ctctgtaccg
    18421 ggcctttcgc taagggaaga tgtatgtcgt tacttgcaca actggatcaa aaaatcgctg
    18481 ctaacggtgg cctgattgtc tcctgccagc cggttccgga cagcccgctc gataaacccg
    18541 aaatcgtcgc tgccatggca ttagcggcag aacaggcggg cgcggttgcc attcgcattg
    18601 aaggtgtggc aaatctgcaa gccacgcgtg cggtggtgag cgtgccgatt attggaattg
    18661 tgaaacgcga tctggaggat tctccggtac gcatcacggc ctatattgaa gatgttgatg
    18721 cgctggcgca ggcgggcgcg gacattatcg ccattgacgg caccgaccgc ccgcgtccgg
    18781 tgcctgttga aacgctgctg gcacgtattc accatcacgg tttactggcg atgaccgact
    18841 gctcaacgcc ggaagacggc ctggcatgcc aaaagctggg agccgaaatt attggcacta
    18901 cgctttctgg ctataccacg cctgaaacgc cagaagagcc ggatctggcg ctggtgaaaa
    18961 cgttgagcga cgccggatgt cgggtgattg ccgaagggcg ttacaacacg cctgctcagg
    19021 cggcagatgc gatgcgccac ggcgcgtggg cggtgacggt cggttctgcc attacgcgtc
    19081 ttgaacacat ttgtcagtgg tacaacacag cgatgaaaaa ggcggtgcta tgaccacgtt
    19141 ggcgattgat atcggcggta ctaaacttgc cgccgcgctg attggcgctg acgggcagat
    19201 ccgcgatcgc cgagaacttc cgacaccagc cagccagact ccgcaggcgt tgcgtgatgc
    19261 cttagccgcg ttagtcgccc cgttgcaagc tcatgcgcag caggttgcca tcgcttcaac
    19321 cggaattatt cgcgacggca gcttgctggc gctgaatccg cataatcttg gtggattgct
    19381 acactttccg ttagtcaaaa cgctggaaca actttccaat ttgccgacca ttgccattaa
    19441 cgacgcgcag gccgcagcat gggcagagta tcaggcgctg gaaggcgata taaccgatat
    19501 ggtgtttatc accgtttcca ctggcgtcgg cggcggtgta gtgagcggcg gtaaactgct
    19561 taccggccct ggcggtctgg cggggcacat cgggcatacg cttgccgatc cacacggccc
    19621 agtctgcggc tgtggacgca caggttgcgt ggaagcgatt gcttctggtc gcggtattgc
    19681 agcggcagcg caaggtgaac tcatgggcgc ggatgcgaga actattttca cacgcgccgg
    19741 gcagggcgac gagcaggcgc agcaactgat tcaccgctcc gcacatgtgc tggcaaggct
    19801 gatcgctgat attaaagcca caactgattg ccagtgcgtg gtggtcggtg gcagcgttgg
    19861 tctggcagaa gggtatctgg cgttggtgga aacgtatctg gcgcaggagc cagcggcatt
    19921 tcatgttgat ttactggcgg cgcactaccg ccacgatgca ggtttacttg gggctgcgct
    19981 gttggcccag ggagaaaaat tatgatgatg ggtgaagtac agtcattacc gtctgctggg
    20041 ttacatcctg cgttacagga cgcgttaacg ctggcattag ctgccagacc gcaagaaaaa
    20101 gcgcctggtc gttacgaatt acagggcgac aatatcttta tgaatgtcat gacgtttaac
    20161 actcaatcgc
//
LOCUS       DAJVUJ010000067        20115 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.067, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000067 DAJVUJ010000000
VERSION     DAJVUJ010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20115
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.067"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..1985
                     /locus_tag="OQQ48_002183"
     CDS             <1..1985
                     /locus_tag="OQQ48_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HCS1952946.1"
                     /translation="VGAFLAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDT
                     FNTEQVEVIKGPSGTDYGHSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVN
                     QVIGDTTAVRLNVMGEKTHDAGRDKVKNERYGVAPSVAFGLGTANRLYLNYLHVTQHN
                     TPDGGIPTIGLPGYSAPSAGTAALNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDI
                     NDNTTIRNTTRWSRVKQDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILT
                     NQTNLTSTFYTSSIGHDVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTR
                     NGANANGQTDTFAIYAFDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCP
                     AGVAKGSPVTTVDTAKSGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQS
                     GSGNSANRTDFKPQKANTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQY
                     GKKRVEGYEISVAGNITPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLW
                     SQYQATDDISVGAGARYIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLN
                     VYNLFDTDYVASINKSGYRYHPGKPRTFLLTANMHF"
     gene            2027..2704
                     /gene="ybiX"
                     /locus_tag="OQQ48_002184"
     CDS             2027..2704
                     /gene="ybiX"
                     /locus_tag="OQQ48_002184"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HCS1952947.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENEEILSLLNLYHNL
                     LREWSEI"
     gene            2778..3044
                     /gene="ybiI"
                     /locus_tag="OQQ48_002185"
     CDS             2778..3044
                     /gene="ybiI"
                     /locus_tag="OQQ48_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HCS1952948.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            3309..3569
                     /locus_tag="OQQ48_002186"
     CDS             3309..3569
                     /locus_tag="OQQ48_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCS1952949.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLTEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(3797..4882)
                     /gene="hcxB"
                     /locus_tag="OQQ48_002187"
     CDS             complement(3797..4882)
                     /gene="hcxB"
                     /locus_tag="OQQ48_002187"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HCS1952950.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCSAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            complement(5023..5985)
                     /gene="ybiB"
                     /locus_tag="OQQ48_002188"
     CDS             complement(5023..5985)
                     /gene="ybiB"
                     /locus_tag="OQQ48_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HCS1952951.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMACCLEATGEAATISDGLARVNQAF"
     gene            complement(6013..8163)
                     /gene="dinG"
                     /locus_tag="OQQ48_002189"
     CDS             complement(6013..8163)
                     /gene="dinG"
                     /locus_tag="OQQ48_002189"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HCS1952952.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGRHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            8283..8765
                     /gene="ybiA"
                     /locus_tag="OQQ48_002190"
     CDS             8283..8765
                     /gene="ybiA"
                     /locus_tag="OQQ48_002190"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HCS1952953.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRALLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            complement(8997..10361)
                     /gene="rhlE"
                     /locus_tag="OQQ48_002191"
     CDS             complement(8997..10361)
                     /gene="rhlE"
                     /locus_tag="OQQ48_002191"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HCS1952954.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSSDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     AAAQ"
     gene            10590..11261
                     /gene="cecR"
                     /locus_tag="OQQ48_002192"
     CDS             10590..11261
                     /gene="cecR"
                     /locus_tag="OQQ48_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HCS1952955.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNTTTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            11264..12259
                     /gene="hlyD"
                     /locus_tag="OQQ48_002193"
     CDS             11264..12259
                     /gene="hlyD"
                     /locus_tag="OQQ48_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HCS1952956.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLKLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQVKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            12252..13988
                     /locus_tag="OQQ48_002194"
     CDS             12252..13988
                     /locus_tag="OQQ48_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS1952957.1"
                     /translation="MNDAVITLNGLEKRFPGMGKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATMIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELASEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            13981..15114
                     /locus_tag="OQQ48_002195"
     CDS             13981..15114
                     /locus_tag="OQQ48_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1952958.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            15125..16231
                     /locus_tag="OQQ48_002196"
     CDS             15125..16231
                     /locus_tag="OQQ48_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1952959.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFTRASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIATNYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(16193..16603)
                     /locus_tag="OQQ48_002197"
     CDS             complement(16193..16603)
                     /locus_tag="OQQ48_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HCS1952960.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            16736..17487
                     /locus_tag="OQQ48_002198"
                     /pseudo
     CDS             16736..17487
                     /locus_tag="OQQ48_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
     gene            17484..18725
                     /gene="clsB"
                     /locus_tag="OQQ48_002199"
     CDS             17484..18725
                     /gene="clsB"
                     /locus_tag="OQQ48_002199"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HCS1952961.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNKLTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWHPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            18725..19681
                     /locus_tag="OQQ48_002200"
                     /pseudo
     CDS             18725..19681
                     /locus_tag="OQQ48_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
     gene            complement(19717..>20115)
                     /locus_tag="OQQ48_002201"
     CDS             complement(19717..>20115)
                     /locus_tag="OQQ48_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BAX inhibitor (BI)-1/YccA family protein"
                     /protein_id="HCS1952962.1"
                     /translation="IIIVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVI
                     IVNAALMSERPIWVISCLVIVLWPGIISHGRNKLLELAGKCHSEELWSPVRCAFTGAL
                     TLYYYFIGFFGILAAIAITLVWQRHTRFFH"
BASE COUNT         4726 a         5445 c         5300 g         4644 t
ORIGIN      
        1 gcgtgggtgc gtttttggcg ggtgagaacg gtaactccac cactggcgac gccatttata
       61 tgcgtggtgc cgatacctct aacagtattt atattgatgg cattcgcgat atcggcagcg
      121 tctcgcgcga caccttcaat accgagcagg tcgaagtgat taaagggccg tccggcaccg
      181 actacgggca cagcgcaccg acgggctcga tcaatatgat cagcaaacag ccgcgcaatg
      241 attccggcat tgacgcctcc gccagtattg gcagcgcctg gttccgccgc ggcacgctgg
      301 acgtcaatca ggtcattggt gataccaccg cggtgcgcct gaatgtaatg ggcgaaaaaa
      361 cgcacgatgc cggacgcgac aaagtcaaaa atgagcgtta cggcgtcgcc ccttctgtcg
      421 cttttggcct tggtacagcg aatcgtttgt atcttaatta tctgcatgtc acccagcaca
      481 acacgccaga cggcggcatt ccgaccatcg gtttgccggg ctattctgcc ccatctgcgg
      541 gaacggcggc cctgaatcat tccggaaaag ttgatactca taacttttac ggcacggatt
      601 ccgattacga cgattcgacc accgacaccg ccaccatgcg ttttgagcac gacatcaacg
      661 ataacaccac cattcgcaat actacccgtt ggtcgcgcgt aaagcaggat tacctgatga
      721 cggcgattat gggcggggcg tcgaatatta ctcagcccac cagcgatgtg aatagctgga
      781 cctggtcacg cacggcgaat accaaagatg tgagtaataa aattctcacc aaccagacca
      841 acctgacctc gacgttctat accagttcta tcggtcatga tgtcagtacc ggcgtggaat
      901 ttacccgtga aacgcagact aactacggcg ttaatccggt gacgttaccc gcggtaaata
      961 tttatcatcc tgacagcagc attcatcccg gcggcctgac gcgcaacggc gcaaacgcca
     1021 atggtcagac ggataccttc gcaatttacg cctttgatac gctgcaaatc acccgtgatt
     1081 ttgagctgaa cggcgggatc cgtctggata attatcatac tgaatatgac agtgccaccg
     1141 cctgcggcgg cagcggacgc ggtgccatca cctgcccagc tggtgtggca aaaggttctc
     1201 cggtcaccac cgtcgacacc gccaagtcgg gcaatctggt gaactggaaa gccggggcgc
     1261 tgtatcacct gacggaaaac ggtaatgtct atattaacta tgccgtttcc cagcagcctc
     1321 cgggcggcaa caacttcgcc cttgcgcagt ctggcagcgg taacagtgcc aaccgcacag
     1381 attttaaacc gcaaaaagcc aacaccagcg agattggcac caaatggcag gttctggata
     1441 aacgcctgtt gctcaccgcc gcactgttcc gtactgatat cgaaaatgaa gttgagcaaa
     1501 atgatgacgg aacttactcg caatacggta agaaacgtgt cgaaggctat gagatatccg
     1561 tggccgggaa tatcactccc gcgtggcagg tgattggcgg ctatacccag caaaaagcaa
     1621 ccatcaaaaa cggcaaagat gttgcccagg atggttcctc atcgctgccg tataccccgg
     1681 agcacgcctt caccttatgg agccagtatc aggcaaccga tgatatctct gttggcgcgg
     1741 gcgcacgcta tatcggcagt atgcataaag gttcagacgg cgcggtggga acgccagcgt
     1801 ttaccgaagg ttactgggtc gccgatgcca aactggggta tcgggttaat cgcaatctcg
     1861 acttccagct aaacgtttac aacctgtttg ataccgatta cgtcgcctca atcaacaaga
     1921 gcggctaccg ttatcacccg ggcaagccaa gaaccttctt gctcacagcc aatatgcatt
     1981 tctgattcag atgtggggcg caggccccac tttttggaga aattgtatga tgtaccacat
     2041 tcccggcgtg ttatcgccac aggacgtcgc tcgttttcgc gaacaactgg aacaagccga
     2101 atgggtggat ggacgcgtca ccaccggcgc acaaggtgcg caagttaaga acaatcaaca
     2161 ggtagacacc cgcagcacgt tatacgccgc cctgcaaaat gaggtgctga acgcggttaa
     2221 ccaacatgct ttattctttg ccgcggcctt gccgcgtacc ctttccacgc cgctgtttaa
     2281 tcgctatcag aacaatgaaa cctatggttt tcatgtggat ggcgcagtac gcagccatcc
     2341 acaaaacggc tggatgcgca ctgacctttc tgccacgctg tttttaagcg atccacaaag
     2401 ctacgacggc ggcgaactgg tcgttaatga caccttcgga caacatcggg taaaactccc
     2461 ggcaggtgat ctcgtgttgt atccctccag cagcctgcat tgcgtgacac ccgtaacccg
     2521 cggcgtacga gtggcatcat ttatgtggat ccagtcgatg atccgcgatg ataaaaagcg
     2581 cgccatgctg tttgaactag acaaaaatat tcagtcgctg aaaagccgct atggtgaaaa
     2641 tgaagagatc ctgtcgctgc tcaatcttta tcataatctg ctgcgggaat ggtcggagat
     2701 ctgacgcttg aactgcccag gttcgtccct atattagcta cacttaactg tacaagcatt
     2761 gatatgggga ggacgatatg gcatccggtt gggctaacga tgacgccgtc aacgaacaga
     2821 tcaacagtac aattgaagat gcgattgccc gcgctcgggg tgaaattccg cgtggcgaaa
     2881 gcctgtatga atgtgaagag tgcggtgccc ccatcccgca agctcgtcgg gaagccattc
     2941 ctggcgtgcg cttatgtatt cattgtcagc aggagaaaga tttacaaaaa ccagcgtata
     3001 caggatataa tcgcagaggt tcgaaagaca gccagttacg ttaactacaa ctggcgagat
     3061 gcatagcgag tataatttgt attttgcgtg gcattccgtg cctttaacgc tgctgtgact
     3121 gccacgcggc ataaaacaag tccataagta caacaaataa atggtttatc agtatgttag
     3181 atgtcaatca atttatttga acaaggcggt caattctctt cgattttatc tctcgaaaaa
     3241 aaacgtgata ctcatcacat cgacgaaaca acgtcactta tacaaaaatc acctgcgaga
     3301 gattaattat gaaaactatc aatactgttg ttgctgctat ggctctttca actctatcat
     3361 ttggcgtgtt cgcggcggaa ccggtaacgg catcccaggc acagaacatg aataaaatcg
     3421 gcgtggtttc tgccgatggc gcatccaccc tcgatgccct ggaagcgaaa ctgactgaga
     3481 aagccgcggc agccggtgcc agtggataca gcatcacttc tgccaccaac aacaataaat
     3541 taagcggtac tgcggtaatt tataagtaat tcgtaggacg gataaggcgt ttacgccgca
     3601 tccggcaaca gatgcctgat gcgacgctac cgcgtcttat caggcctaca aaatccgaac
     3661 cgttggacgg atcaggcggt tacgctacgt ccagcacaag tatttccctg tctgtttgcc
     3721 gacagacgca tatgctctaa ccctcattga tcctatatta cccttgtttg cccgtccgtt
     3781 actggacggg ctttttttag ctggctaact gctgacagaa agcctgcaac gtctcttccg
     3841 acataccaat ctgccgcgcc gcatcacaaa tggcctgcca gcttcccgca tccaatggaa
     3901 tcccctgctc ctgccgttcg cgacgcgtgt tcacttccca ctcgcccggt agcaaaatcg
     3961 gcttatcgtc atcatgcggc gaggctttca cccactcggc aaaggcttca gtctgcgcgc
     4021 tacaatccgg cgcgccgaac agttccgggt tgatgatgat agtggtcatg cagttaagaa
     4081 tggcatcggg actggtttgt aacgtttcct gatgcgtcgt tttaccgcca gaaagcgccc
     4141 cgccaagaat ttcacacatc gctgcaaggg cgtagccttt atgttcggca aaggtcaaca
     4201 gcgaccccaa cggcgactcc tgcattaccg ccggattggt cgtcggcacg ccgttaacgt
     4261 caatcaggca acctggcggc acggggacgc ctttatgcca ggcgacgcga gttttaccaa
     4321 atgcgatggc gctggtggcg taatcaagca acagcgggaa attatcttta cgagggaaaa
     4381 ccacacagaa cggattggtg ccaaagcggc tgtcgcgacc gtggaacggt gcgaccattg
     4441 gaataccgac cacgctaaca aagtggatag agacaaaccc cgccgctgca cactgctccg
     4501 cccagtaacc gatacggccg atatgatgcg agttatgtag cgccacggca gcaataccgt
     4561 gctgatgcgc tttctcaatc cccagcgcca tcgcttcgtg tgccgcgacc tgaccgaatg
     4621 cgcgatcgcc atcgagcgtg actgccgccc ccgcctcttt aacgattttg gcatgatggt
     4681 taatttgcag atgcccctga ctccaggaac gcacatagct tgggatcatg ccaataccat
     4741 gagaatcatg ccctgccagg tttgccgcga ttaaatgatc ggccactaat ttcgcttctt
     4801 gttcctcgct acccatctga cgaaatacag cctgaataaa actgtgcagc gtctgagcat
     4861 caaagcgatg accactttcc atggttaact cctgtttatg ttatgtgttt gttgtttttt
     4921 tatgctgcac gccgggcgcg ggaaggtcaa taaagatgca ggaatttcat attgcggtgg
     4981 ggtaaaagcc ggatgacatg gctcatccgg ctgagaaaag aattaaaatg cctgattaac
     5041 gcgcgccagg ccgtcgctga tagttgccgc ttcacccgta gcctccaggc agcaagccat
     5101 ctggattttc agcgattcgg gaatcggttc gctgccagca aggcaacgct caatccactg
     5161 cgccgtggtt tctgggtctt ttgcttgtgg cagtaactcg ctaccagcag tgtcctgttt
     5221 ttcatacagc acccgcatcc cttcacggtc aatgagattg atctgcgggc agcgctgcgg
     5281 attagcatac acttcgcctt cagtaccatg cattagtagc gcccgcccgc cgatatcgct
     5341 aaagaacttc gcgactcgcc cgatgtattc cggatgcgaa acgctggaaa gacgcagcgc
     5401 ctcaccttcg gcaaatggcg tcgccagttt cgccagggta tgtgcactgt tacgcacgcc
     5461 catccgccag cgcatcgcca gttgtttttc cagcggcggg caaaacgcgc cgacaggcat
     5521 aaacaccggt tgatgttcgt cgagcttcgc ctgcgcctgc ccgccgtgca acgttggtgt
     5581 aatacccatc agttcgaaaa tggtttctgt cagcacgcga gttggatctt cgctaacccc
     5641 gtgaaccacc acaggaaaac cgagtttgtg caggagaata gccaacaacg gcgtcaggtt
     5701 ggcctgttta cgcgcgccgt tgtaactggg gatgacaatc ggcatcggct tgcctgcagg
     5761 cggcgtcagc ttgatggtgt gattttgcat ggcttcgtaa aagccgagca tctctgcttc
     5821 cccttcccct ttgatacgta gcgcaatcaa tacgccgccc aactcaaggt cagggacttc
     5881 accattgagc atatgggcat acagaccgcg cgcagtatcc cggtctaaat cgcgcgcgtg
     5941 gtttttcccg cgcccgatct ctttaatgat tttgcgatag tccatttacg actccttacc
     6001 tgactcacat cattaacgcc gccgacggcg tggggatttc gttttttctt tctttttaac
     6061 tataccttca gggacttccg gttgctctat cggaaatacc ggtaatgcat ccagtagtcg
     6121 cttgccataa tttttggtca gcaagcgttt gtcgtagata accacttcgc cccagcaacc
     6181 gtggcttcga atcagtcgcc caacctgctg aatcaggtta aacgaggcgc tcggcaggct
     6241 ttgcacctca aacggatagc ggttgaggct tttcagccat tccccttcgg tgatcaccac
     6301 cgggctgtcg atgggcggaa aagcgatttt gtggatatgc acctggctga gcagatcacc
     6361 tttcaaatca agcccttcgg caaatgactg taagcccacc agcacactac gctcaccgtt
     6421 ggcaacgcgt ttgcggtgca gttcaactaa gcgataacgc ggctgatcgc cctgcaccag
     6481 cagcatcagg cgtaaatccg tcacatagtc gagaaagcgc tgcatcgccc gtccgctggc
     6541 aaacagtacc aacataccga gatgtttttt gctctccacc tgctcacgga aaaaggccgc
     6601 catttcggca atatgctgct cttcgttgtc gatggaaggc tcaaagcgca tccggggaat
     6661 aacaattttg ccctgttcgc agtggttaaa gggggaatcc agcgccacaa aacggtcgcc
     6721 cgctttctct ttcagcccgc tcatctcctg caaacgcgaa aaactgttca gcgaacgcaa
     6781 ggttgcggag gtgacaataa tgtgcggaat actgcgccac agcagccttt ccagctgatc
     6841 gctgacacgt attcccacgc agtgaaacca gagatgcagc tgcccttcgc gctcttcccg
     6901 cgtcgcccat ttggtcaccg gcgcaccgga agattgcgcc agagacgcca gccgccagag
     6961 tttgctttgc gcctcgaaca tccccaacgc gcggttcatc tgcaaaatca accgatgcag
     7021 acgcacaata tcgtggcggc cggttttctc acttaaatcg ttaagaaata actccgccag
     7081 accacgcagc atctcggtga gttttgccag ccgctggcag atctccagca cttcatcagg
     7141 cagttcgccc atcgcaaaac ggtgctctgc ctcctgcccg gcaggcatgt agagattgag
     7201 aatgttgttt aacgaggcga taagctcata caactcttca caatgcgcat tcaaacgttc
     7261 agggatcgcc agcggtggga tggtcttcgg gcgaaactgc tccatgcagg tagcgaccag
     7321 tttcgtgaac aagtccagct gtagtcgata ccagggcgcg gtgatttcag cgctcatctc
     7381 cagcgcatcc cgggccacat ctggcaggtg atgaccttcg tccagcacca gcagcagatt
     7441 tttcgggtca ggcagtacgg cttcgctttc catcgccgcc atcaccagcg catggtttgc
     7501 caccaccact tccgcttcct gaatctcccg acgagcaaca aaaaacgggc attcacgata
     7561 gtagtaacag ttacggttaa ggcagctggc tttgtcggta ctcaaacgac gccagagatc
     7621 atcatctata gcgatatccg tatgatcgcg cagaccatcc catttataag tgtcgagatc
     7681 gcccttcagc ttcgcacaac gtttttgctc ttcctgatta tttggcgtca gttcgtcgtc
     7741 aagaaacgcc agcagatcct gttgcgtggg ttcagtgctg gcgagcgccg tcagattacg
     7801 cggacaaacg tagcgcccac gcccgaaagc ggcagtgaat ttaagatcgg gaatgatctt
     7861 tttcagcagc ggtaaatctt tgctgtaaat ctgatcctgc aacgccacgt tggcggtact
     7921 caccaccagc gttttttgct cttcgcgggc aatggcgatg ccggggatca aataggagag
     7981 cgttttccca acgccggtgg gggcttcaat cgccagatgc cgcccttctt ctccggccag
     8041 cgttttggcg acgtccgcaa tcatctgccg ctgcggcgca cggggaataa agtcggggat
     8101 ctgttcctga agcgccttat accaggcggc aatttgcgct ttaagcgcgg cggttaatgc
     8161 catgagaaaa cctgaaatac tgtataaaca gccaatattg tggcattttt cggttttcag
     8221 gggtaatggt ttttctgttt ctggaaagcg gctcgcaata accaccaaca taatgcggtt
     8281 taatgcccgt tcgagcacaa aggatccaac acgtcatgca agacaccatc ataaatttct
     8341 acagcaccag cgatgactac ggtgattttt ctaactttgc cgcctggccc atcaaggttg
     8401 acgggaaaac ctggcccacc tccgaacact attttcaggc acaaaaattc cttgatgaaa
     8461 aataccgcga agagatccgt cgggtttctt cccccatggt cgccgctcgc atggggcgta
     8521 atcgttccaa gccacttcgt aaaaactggg agtcggttaa agaacaggtg atgcgtaaag
     8581 cacttcgtgc taaattcgaa cagcatgcag aactgcgtgc gctcttgctg gcaaccgcgc
     8641 ccgcaaaact ggttgagcat acggaaaacg atgcttactg gggagacggt ggtaatggta
     8701 agggcaagaa tcgactgggc taccttttaa tggagttgcg cgaacaattg gctatagaga
     8761 agtaacgtct cggtggctta tgggcggtaa aacagataaa ttttttcccg tgttctcaaa
     8821 ttgctgacaa gtgcttgttg tgaaatgccg gatgcggcgt aaacgcctta tccggcctac
     8881 aaaagcatgc aaattcaata tattgcagag ttaacgtagg cctgataagc gtagcgcatc
     8941 aggcagtttt gcgtttgttc atcagcctga tgccgggcat agcccggcat aaaagattac
     9001 tgcgcagcgg caggtttacg cggacggcgg cgacgttgtt gttcgcctgc cggtttggca
     9061 tcgccgaggc ggcgagacgg tttttctgca ggtttcgcgc ttgcagattt tgcgccacct
     9121 tccccacggc gtggttgctg ttgaccgcga ccaccacctt gcccacgacc gccgccgcca
     9181 cgttgctggc gaccgttctg gatcggttcg gctttgattg acgggtccgg ctcatagccc
     9241 ggaatcgcaa tgcgcgggat ctcttttttc agcagttttt cgatatcacg cagcagtttg
     9301 tgttcatcaa cacacaccag cgacaacgct tcaccggtag cagccgcacg accggtacgc
     9361 ccgatacggt ggacataatc ttccggtacg tttggcagtt catagttgac gacgtgcggc
     9421 aactcttcga tatccagacc gcgcgcagcg atatcggtcg ccaccagtac gcgaatgtca
     9481 ctcgatttaa aatcagccag cgcacgggta cgcgcacctt gcgatttatt cccgtggatc
     9541 gccgcactac ggatgccatc tttattgagc tgctcagcca gatggttagc gccgtgtttg
     9601 gtacgggtaa acaccagcac ctgctgccag ttccctttac caatcatgtg cgacagcaat
     9661 tcgcgtttgc gtttcttatc gacaaagtga acgtgctggg tcacctgatc agacgcggta
     9721 ttgcggcgtg ccacttcgat ttccagcggg ttgtgcaaca gtttttccgc cagggcttta
     9781 atatcgtcag agaaggtcgc agagaacagc aggttctggc gtttcgctgg cagttttgtt
     9841 aacacgcgac ggatatcgtg gataaagccc atgtcgagca tgcggtcagc ttcatcgagg
     9901 acgaggattt caacctgatc cagcttcact gcattttgat gttccaggtc cagcaaacgt
     9961 cccggtgttg ccaccagaac atcaacgccg ccgcgcagct tcatcatttg cgggttaatg
    10021 ctgacaccgc caaacaccac cagcgaacga atgttcaaat atttgctgta atcacggacg
    10081 ttttcgccaa tctgcgcagc cagttcacgg gtcggggtaa gaatgagcgc acgtaccgga
    10141 cgacgccctt tggcgtgcgg ctggcgagtg atcaggtgtt gcaacagcgg cagcgtaaag
    10201 cccgctgttt taccggtacc ggtctgggcg ctagccatca ggtcgcggcc ttccagcacc
    10261 gcagggatcg cctgctgctg aataggggtg ggttcacggt aaccctgctc ggcaacggcg
    10321 cgcaggatat cagggcttaa acccaaagaa tcgaaagaca taactactcc gaaccgcccc
    10381 gaccgttacc ggtgtagttt tcagggagat actgaaaagg aaaatgacaa aaaccacaat
    10441 gtcatgaggg agcggagtgt agcagttttt gtgacgcagc gcataaatta tccctgtgac
    10501 tggcgttgct aaaattctta gccatactgg acaactcccc tatggagtca taatcttaat
    10561 caatcatttg attaagaatg gactgtgcga tgaataatcc tgccatgaca atcaagggtg
    10621 aacaggcgaa aaaacagctg attgctgccg cactggcgca gtttggtgaa tatggaatga
    10681 acaccaccac tcgcgagata gccgcccagg ccgggcagaa tatcgctgcc atcacctact
    10741 acttcggttc gaaagaagat ttgtacctcg cctgcgccca gtggattgcc gattttattg
    10801 gcgagcagtt ccgtccgcat gccgaggaag ccgaacgctt gttcgcgcaa ccacagcctg
    10861 atcgggctgc catccgtgaa ctgatccttc gcgcctgcag gaacatgatt aagctgctca
    10921 cccaggatga caccgtcaac ctcagcaagt ttatctcccg tgagcagctc tctcccacgg
    10981 cagcctacca cctggtgcac gaacaggtga ttagtccgct acacagccac ctgacacgcc
    11041 tgattgccgc ctggaccggc tgcgacgcca atgacacccg catgatcctt catacccatg
    11101 cgttgattgg tgagattctg gcgttccgtc ttggcaaaga aacaattctg ttacgtaccg
    11161 gctggaccgc gttcgatgaa gaaaagaccg aactgatcaa ccagacggtg acctgtcata
    11221 tcgacctgat tttgcaagga ttatcgcaaa ggagtttgta gtgatgaaaa aacctgtcgt
    11281 gatcggactg gcggtagtgg tacttgccgc cgtggttgcc ggaggctact ggtggtatca
    11341 aagccgccag gataacggcc tgaagctgta tggcaacgtg gatattcgta cggtgaatct
    11401 tagtttccgt gttggggggc gcgttgaatc gctggcggtg gacgaaggtg atgcgatcaa
    11461 agcgggccag gtgctgggcg aactggatca caagccgtat gagattgccc tgatgcaggt
    11521 gaaagcgggc gtctcggtgg cacaggcgca gtatgacctg atgcttgccg ggtatcgcga
    11581 tgaagaaatc gctcaggccg ccgcagcggt gaaacaggcg caagccgcct atgactatgc
    11641 gcagaacttc tataaccgcc agcaaggact gtggaaaagc cgcaccattt cggcaaatga
    11701 cctggaaaat gcccgctcct cgcgcgacca ggcacaggca acgctgaaat cagcacagga
    11761 taaattgcgt cagtaccgtt ccggtaaccg tgaacaggac atcgctcagg cgaaagccag
    11821 cctcgaacag gcgcaggcgc aactggcgca ggcggagctg aatttacagg actcaacgtt
    11881 gatagcaccg tctgatggca cactgttaac gcgcgcggtg gagccaggca cggttctcaa
    11941 tgaaggtggc acggtgttta ccgtttcact aacgcgtccg gtgtgggtgc gcgcttatgt
    12001 tgatgaacgg aatcttgacc aggcccagcc ggggcgcaaa gtgctgcttt ataccgatgg
    12061 tcgcccggac aagccgtatc acgggcagat tggtttcgtt tcgccgactg ctgaatttac
    12121 cccgaaaacc gtcgaaacac cggatctgcg taccgacctc gtctatcgcc tgcgtattgt
    12181 ggtgaccgac gccgatgatg cgttacgcca gggaatgccg gtgacggtgc aattcggtaa
    12241 cgaggcagga catgaatgat gccgttatca cgctgaacgg cctggaaaaa cgctttccgg
    12301 gcatgggcaa gcccgccgtc gcgccgctcg attgtaccat tcacgccggt tatgtgacgg
    12361 ggctggtggg gccggatggt gcaggtaaaa ccacgctaat gcggatgctg gcgggattac
    12421 tgaaacccga cagcggcaat gccacgatga ttggctttga tccaatcaaa aacgacggcg
    12481 cgctgcacgc cgtgctcggc tatatgccgc agaaatttgg tctgtatgaa gatctcacgg
    12541 tgatggagaa cctcaatctg tacgcggatt tgcgcagcgt caccggcgag gcacgtaagc
    12601 aaacttttgc tcgcctgctg gagtttacgt ctcttgggcc gtttaccgga cgcctggcgg
    12661 gcaagctctc cggtgggatg aaacaaaaac tcggtctggc ctgtaccctg gtgggcgaac
    12721 cgaaagtgtt gctgctcgat gaacccggcg tcggcgttga ccctatctca cggcgcgaac
    12781 tgtggcagat ggtgcatgag ctggcaagcg aagggatgtt aatcctctgg agtacctcgt
    12841 atctcgacga agccgagcag tgccgtgacg tgttactgat gaatgaaggc gagttgctgt
    12901 atcaggggga accaaaagcc ctgacacaaa ccatggccgg acgcagcttt ctgatgacca
    12961 gtccacacga gggcaaccgc aaactgttgc aacgcgcctt gaaactgcca caggtcagcg
    13021 acggcatgat tcaggggaaa tcggtacgtc tgatcctcaa aaaagaggcc acaccagacg
    13081 atattcgcca tgccgacggg atgccggaaa tcaacatcaa cgaaactacg ccgcgttttg
    13141 aagatgcatt tattgatttg ctgggtggtg ccggaacctc agaatcgccg ctgggtgcaa
    13201 tattgcatac ggtagaaggc acacccggcg agacggtgat cgaagcgaaa gaactgacca
    13261 agaaatttgg tgattttgcc gccaccgatc acgtcaactt tgccgttaaa cgtggggaga
    13321 tttttggttt gctggggcca aacggcgcgg gtaaatcgac cacctttaag atgatgtgcg
    13381 gtttgctggt gccgacttcc ggccaggcgc tggtgctggg gatggatctg aaagagagtt
    13441 ccggtaaagc gcgccagcat ctcggctata tggcgcaaaa attttcgctc tacggcaacc
    13501 tgacggtcga acagaattta cgctttttct ctggtgtgta tggcttacgc ggtcgggcgc
    13561 agaacgaaaa aatctcccgt atgagcgagg catttggcct gaaaagtatc gcctcccacg
    13621 cgaccgatga actgccatta ggttttaaac agcggctggc gctggcctgt tcgctgatgc
    13681 atgaaccgga cattctgttt ctcgacgaac caacgtccgg cgttgatccc ctcacccgcc
    13741 gcgaattttg gctgcatatc aacagcatgg tagagaaagg cgtcacggtg atggtcacca
    13801 ctcactttat ggatgaagca gaatattgcg accgcatcgg cctggtgtac cgtgggaaat
    13861 taattgccag cggcacgccg gatgatttga aagcgcagtc ggccaacgac gagcaacccg
    13921 atcccaccat ggagcaagcc tttattcagt tgatccacga ctgggataag gagcatagca
    13981 atgagtaacc cgatcctgtc ctggcgtcgc gtacgggcgc tgtgcgttaa agagacgcgg
    14041 cagatcgttc gcgatccgag tagctggctg attgcggtag tgatcccgct gctactgctg
    14101 tttatttttg gttacggcat taacctcgac tccagcaagc tgcgggtcgg gattttactg
    14161 gaacagcgta gcgaagcggc gctggatttc acccacacca tgaccggttc gccctacatc
    14221 gacgccacca tcagcgataa ccgtcaggaa ctgatcgcca aaatgcaggc ggggaaaatt
    14281 cgcggtctgg tggttattcc ggtggatttt gcggaacaga tggagcgcgc caacgccacc
    14341 gcaccgattc aggtgatcac cgacggcagt gagccgaata ccgctaactt tgtacagggg
    14401 tatgtcgaag ggatctggca gatctggcaa atgcagcgag cggaggacaa cgggcagact
    14461 tttgaaccgc ttattgatgt acaaacccgc tactggttta acccggcggc gattagccag
    14521 cacttcatta tccccggtgc ggtgaccatt atcatgacgg tcatcggcgc gattctcacc
    14581 tcgctggtgg tggcgcgaga atgggaacgc ggcaccatgg aggctctgct ctctacggag
    14641 attacccgca cggaactgct gctgtgtaag ctgatccctt attactttct cgggatgctg
    14701 gcgatgttgc tgtgtatgct ggtgtcagtg tttattctcg gcgtgccgta tcgcgggtcg
    14761 ctgctgattc tgttttttat ctccagcctg tttttactca gtaccctagg gatggggctg
    14821 ctgatttcca cgattacccg caaccagttc aatgccgctc aggtcgccct gaacgccgct
    14881 tttctgccgt cgattatgct ttccggcttt atttttcaga tcgacagtat gcccgcggtg
    14941 atccgcgcgg tgacgtacat tattcccgct cgttatttcg tcagcaccct gcaaagcctg
    15001 ttcctcgccg ggaatattcc agtggtgctg gtggtaaacg tgctgttttt gatcgcttcg
    15061 gcggtgatgt ttatcggcct gacgtggctg aaaaccaaac gtcggctgga ttagggagaa
    15121 gagcatgttt catcgcttat ggacgttaat ccgcaaagag ttgcagtcgc tgctgcgcga
    15181 accgcaaacc cgcgcgattc tgattttacc tgtgcttatt caggtgattc tgttcccgtt
    15241 cgccgccacg ctggaagtga ctaacgccac catcgccatc tacgatgaag ataacggcga
    15301 gcattcggtg gagctgaccc aacgttttac ccgcgccagc gcctttactc atgtgctgct
    15361 gctgaaaagc ccacaggaga tccgcccaac catcgacaca caaaaggcgt tactcctggt
    15421 gcgtttcccg gctgacttct cgcgcaaact ggataccttc cagaccgcgc cgttgcagtt
    15481 gatcctcgac gggcgtaact ccaacagtgc gcaaattgcc accaactacc tgcaacagat
    15541 cgtcaaaaat tatcagcagg aactgctgga aggaaaaccg aaacctaaca acagcgagct
    15601 ggtggtacgc aactggtata acccgaatct cgactacaaa tggtttgtgg tgccgtcact
    15661 gatcgccatg atcaccacta tcggcgtaat gatcgtcact tcactttccg tcgcccgcga
    15721 acgtgaacaa ggtacgctcg atcagctact ggtttcgccg ctcaccacct ggcagatctt
    15781 catcggcaaa gccgtaccgg cgttaattgt cgccaccttc caggccacca ttgtgctggc
    15841 gattggtatc tgggcgtatc aaatcccctt cgccggatcg ctggcgctgt tctactttac
    15901 gatggtgatt tacggtttat cgctggtggg attcggtctg ttgatttcat cactctgctc
    15961 gacacaacag caggcgttta tcggcgtgtt tgtctttatg atgcccgcca ttctcctttc
    16021 cggttacgtt tcgccggtgg aaaacatgcc agtatggctg caaaacctga cgtggattaa
    16081 ccctattcgc cactttacgg acattaccaa gcagatttat ttgaaggatg cgagtctgga
    16141 tattgtgtgg aatagtttgt ggccgctact ggtgataacg gccacgacag ggtcagcggc
    16201 gtacgcgatg tttagacgta aggtgatgta acttcttatc tttcgccagc aaagacacta
    16261 ccgccgggcc ggcaaggatt gccagccctg caattgccag caaaaagttg ttttccagta
    16321 cgcgggacaa cagccacagc acaatcagcg ctgcgccaaa ataccaggtc gtggtcagct
    16381 cttccaacac gtctgccacc tcacgccagc gttgctcggg gtggcgctga aacaccaaca
    16441 tcatcactac cgtgacacaa tacagtgcgc ataatcccac gccgacgtga accagagtct
    16501 tactcatcca gcccaccacc agcagcgcca ctaccagtaa atgcaacata atctgccagc
    16561 aacttagacc cgttgcgaca cgaacacgtt gttgccactt catggcttct ctcctgaaga
    16621 atttatctct acttactcag agtaccgaac tttacggaaa attccgtaag accgcacctt
    16681 tttgtgacgc cgactacact atttcaatga gataagaggc aaagacagga aaattatgcc
    16741 cgatcaaaca caacaatttt cgttcaaggt gctcaccatc aacattcaaa aaggttttac
    16801 cgcgtttaac cgacgcttca ttttgccgga acttcgcgac gcggtgcgta ccgtcagcgc
    16861 cgatattgtt tgcctgcagg aagtgatggg cgcgcacgaa gttcatccgc tgcatgtgga
    16921 aaactggccc gatacctcgc actacgagtt tctcgccgac actatgtgga gcgattttgc
    16981 ctacggtcgc aatgccgtat acccggaagg gcatcacggc aacgccgtac tgtcgcgtta
    17041 tcccattgaa cattatgaga atcgcgatgt ttcggtcgat ggtgcggaaa agcgcggcgt
    17101 gctctactgc cgcattgtgc cgccgatgac cggaaaagcg attcatgtga tgtgcgtaca
    17161 tctgggcctg cgtgaggcgc accgtcaggc gcagcttgcg atggggtgaa tgagctaccg
    17221 gacggcgaac cggtattggt ggcgggtgat ttcaatgact ggcggcaaaa agctaatcat
    17281 ccgttaaaag tgcaggccgg actggatgag atttttaccc gcgcccacgg acgcccggcg
    17341 cgcacgtttc cggtgcaatt tcctctacta cgactggaca ggatctacgt caaaaatgcc
    17401 agcgccagcg cgccaaccgc gttgccgctg cggacatggc gacacctttc tgatcatgcc
    17461 cctttaagtg cggagattca tttatgaaat gtagctggcg cgaaggcaat aagatccagt
    17521 tgctggaaaa cggcgagcaa tattatcccg cggtgtttaa ggcgattggc gaggcacaag
    17581 aacgcatcat tcttgaaacg tttatctggt ttgaggataa cgtcggcaaa cagctgcatg
    17641 cggcactact ggcagcagcg caacgcggag ttaaagcgga agtcttgctg gatggctacg
    17701 gttcgccgga tctcagcgat gagtttgtca ataaactgac ggcagctggc gtagtgttcc
    17761 gctactacga tccccgccct cgcctttttg gtatgcgcac caatgtgttt cgccggatgc
    17821 atcgcaaaat tgtggtgatc gacgcgcgta tcgcctttat tggcgggctg aattactccg
    17881 ccgagcatat gtccagctac ggtccagagg ctaaacagga ttacgcggta cgccttgaag
    17941 ggccgattgt cgaagatatt ctccagtttg agctggaaaa cctgcctgga cagagcgcgg
    18001 cacggcgctg gtggcgacgt catcacaaag cggaagagaa ccgccagccg ggagaagcgc
    18061 aggtattgct ggtctggcgc gataacgaag aacatcgcga tgatattgaa cgccattatc
    18121 tgaaaatgct cactcaggcg cggcgggaag tgattatcgc caacgcctac ttcttccccg
    18181 gctatcgatt tttacacgcc ttgcgtaaag cggcacggcg cggggtgcgg atcaaactga
    18241 tcattcaggg cgaaccggat atgccgattg tcagagtcgg tgcgcgcttg ctgtataact
    18301 atctggttaa aggcggcgtt caggtttttg agtaccgccg ccgcccgctc cacggcaaag
    18361 tggcattgat ggacgatcac tgggcgacag tagggtccag taatctcgat ccgctcagtt
    18421 tgtcactgaa tctcgaagca aatgtcatca tccacgatcg tcattttaac cagacgctgc
    18481 gcgataatct gaacggcatt attgccgcag attgtcagca ggtggatgaa accatgctgc
    18541 ccaaacgcac ctggtggaac ctgaccaaaa gcgtgctggc gttccacttt ttacgccact
    18601 tcccggcgct ggttggctgg catccggcac acacaccacg tctgacgcag gttgatcccc
    18661 ccgcacaacc gacaatggaa acgcaggatc gggtagaaac tgaaaacacg ggggtaaaac
    18721 cctgatgagt aaatcacacc cgcgctagcg cttagcaaag aagatcctca cctggctgtt
    18781 ttttatcgcg gtgattgtgt tgctggtggt ctacgccaaa aaagtggact gggaagaggt
    18841 ctggaaggtc atccgcgact acaatcgcgt tgcgctgctt agtgcggtcg ggctggtggt
    18901 cgtcagctat ctgatttacg gctgctatga cctgctcgcc cgcttttact gcggtcacaa
    18961 actggcgaag cgccaggtga tgctggtgtc gtttatctgc tacgccttca acctgacgct
    19021 cagtacctgg gtcggcggca ttggtatgcg ctatcgtttg tactcccgac tagggttacc
    19081 gggcagcact attacgcgga ttttctcgct cagtattacc accaactggc tgggctatat
    19141 tttactggca gggattatct ttaccgcagg cgtggtggag ttgccggatc actggtatgt
    19201 cgctcaaact acgctgcgta ttctcggcat tggcttactg atgattatcg cggtttattt
    19261 gtggttttgc gctttcgcga agcaccgcca tatgaccatc aaagggcaaa agctggtgct
    19321 gccttcatgg aaattcgccc tcactcagat gctgatttcc agcgttaact ggatggtaat
    19381 gggggcgatt atctggctgt tacttggtca aagcgtgaac tatttctttg tactgggcgt
    19441 gttactggtt agtagtattg ctggcgtcat cgtgcatatt ccggcgggga tcggtgtgct
    19501 ggaagcggtg tttatcgcac tactggctgg ggagcatacc tcgaaaggga gcattatcgc
    19561 cgcgctgctc gcttatcgcg tcctgtatta ctttatcccg ctgctgctgg cgctgatctg
    19621 ttacctgcta ctggaaagcc aggcgaagaa gctacgggcg aaaaatgaag cggcgatgtg
    19681 aaatttagtt aagttcttta gtatctgcat tcacgactaa tgaaaaaaac gcgtatgcct
    19741 ttgccagaca agcgttatag ctatcgcggc aagtatccca aagaagccga taaaatagta
    19801 atagagtgtt aatgcacctg taaaagcgca acgaaccgga ctccacaact cttcactatg
    19861 gcattttcct gccagttcaa ggagcttatt tcgtccatgc gagataatgc ctggccataa
    19921 cacaatcact aagcaactta ttacccaaat gggccgttca ctcattaatg ccgcattcac
    19981 gatgattacc agggccaacc ctgtcaacgt catcataatg ataaaacggt gagaaccagg
    20041 atcaacatta aataataaac cggccagcat actgattaaa aacatcgcgc ccgcagtgct
    20101 gaacacaatg ataat
//
LOCUS       DAJVUJ010000068        20028 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.068, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000068 DAJVUJ010000000
VERSION     DAJVUJ010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20028
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.068"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(<1..451)
                     /locus_tag="OQQ48_002202"
     CDS             complement(<1..451)
                     /locus_tag="OQQ48_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCS1952963.1"
                     /translation="MNNTDLIHLIKRFMRNEHKAVEEVIDSPLSEVANLIKILQSCQG
                     KVVFIGVGKSGIIARKLAATFASTGTPSFFVHGTEAVHGDLGMVAKDDVVILISNSGE
                     TAEILATLPSLKKMGNYLISFIRSHHSSLAISCDLSVEIPVKSEADNL"
     gene            complement(420..1628)
                     /locus_tag="OQQ48_002203"
     CDS             complement(420..1628)
                     /locus_tag="OQQ48_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HCS1952964.1"
                     /translation="MDIFNTSISRKGTYCTQWDFCEDRFGVKDVLPFSISDMDLPIPE
                     AIIRTLKKRLEHPILGYSRWQHDDYLNSIVNWYHQQYKTEIQPEWITYSPSVMYSISK
                     AIEILTLNGDNILIFTPVYNAFFDVIKHNGRNMLTTSLMKENGRGYVINWQDFELKIK
                     SAKMVLLCNPHNPTGTVWSEDDLHRIAACCARHNIWLCSDEIHSDFVFNHSFTSALTI
                     KDDKVVVFNSISKTFNVPALTGSYMISTDDNFNHKFRTISRYRDFVNSPSVLNVIATI
                     VAYNECEEWLKSLKVHIASNIQFTLQYLNENIPELIVWPAEGCYFSWVDCSALGYPFD
                     KFYRRLINEGRVGIMAGHVYGTEGEGYLRLNLACSREKLYMGLARLASVIKNINQEKE
                     NEQYGSYPSY"
     gene            complement(1628..3208)
                     /locus_tag="OQQ48_002204"
                     /pseudo
     CDS             complement(1628..3208)
                     /locus_tag="OQQ48_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000810568.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
     gene            complement(3240..3886)
                     /locus_tag="OQQ48_002205"
                     /pseudo
     CDS             complement(3240..3886)
                     /locus_tag="OQQ48_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569552.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            complement(3909..4061)
                     /locus_tag="OQQ48_002206"
     CDS             complement(3909..4061)
                     /locus_tag="OQQ48_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HCS1952965.1"
                     /translation="MIIEKVMNNNCVQASVNGQEVIISGPGVGYNKNMVCRSLSILQT
                     GFLCQK"
     gene            complement(4321..5574)
                     /gene="amiC"
                     /locus_tag="OQQ48_002207"
     CDS             complement(4321..5574)
                     /gene="amiC"
                     /locus_tag="OQQ48_002207"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HCS1952966.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            5806..7137
                     /gene="argA"
                     /locus_tag="OQQ48_002208"
     CDS             5806..7137
                     /gene="argA"
                     /locus_tag="OQQ48_002208"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HCS1952967.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(7199..9025)
                     /gene="recD"
                     /locus_tag="OQQ48_002209"
     CDS             complement(7199..9025)
                     /gene="recD"
                     /locus_tag="OQQ48_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HCS1952968.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     TLTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(9025..12567)
                     /gene="recB"
                     /locus_tag="OQQ48_002210"
     CDS             complement(9025..12567)
                     /gene="recB"
                     /locus_tag="OQQ48_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HCS1952969.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIATVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPKSVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNEKGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDRHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(12560..15448)
                     /gene="ptrA"
                     /locus_tag="OQQ48_002211"
     CDS             complement(12560..15448)
                     /gene="ptrA"
                     /locus_tag="OQQ48_002211"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HCS1952970.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISEQTFADWQQKAANIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTQARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTTEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(15624..18992)
                     /gene="recC"
                     /locus_tag="OQQ48_002212"
     CDS             complement(15624..18992)
                     /gene="recC"
                     /locus_tag="OQQ48_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HCS1952971.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEHEDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPEWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRLWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGLSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDNI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(19005..19328)
                     /locus_tag="OQQ48_002213"
     CDS             complement(19005..19328)
                     /locus_tag="OQQ48_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCS1952972.1"
                     /translation="MSASLKNQQGFSLPEVMLAMVLMVMIVTALSGFQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRLQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(19313..19720)
                     /locus_tag="OQQ48_002214"
     CDS             complement(19313..19720)
                     /locus_tag="OQQ48_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HCS1952973.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAETDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGNIVFSPSGWSDFCPLKERALCQLP"
     gene            complement(19717..>20028)
                     /locus_tag="OQQ48_002215"
     CDS             complement(19717..>20028)
                     /locus_tag="OQQ48_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCS1952974.1"
                     /translation="CIIVQWDANSNGIWDREPVKESDQIGFRLKEHVLETLRGATSCE
                     GKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVLTVNMRAASKSEPQTVVNASYSVTGFN
                     L"
BASE COUNT         5056 a         5347 c         4663 g         4962 t
ORIGIN      
        1 ctaaattatc cgcctcactt ttgacgggga tttcaacaga taaatcacaa cttatcgcaa
       61 gcgatgagtg atgactgcgg ataaaagaga tcaaataatt acccattttt ttcaaactag
      121 gcagtgtcgc caggatttcc gccgtctcac cactgtttga aataagaata acaacatcgt
      181 ctttcgccac catcccaagg tcgccgtgta ccgcttccgt accgtgaaca aaaaacgagg
      241 gagttccggt gctggcaaat gttgcagcaa gttttctggc aataatgcca gatttcccaa
      301 caccaataaa aacaactttt ccctggcaag attgtaatat tttaattaaa ttcgcgactt
      361 ccgaaagggg ggaatcaata acctcttcta ctgctttatg ttcgttacgc ataaaacgtt
      421 taataagatg gataagatcc gtattgttca ttttcttttt cctgattaat atttttaatt
      481 acactcgcaa ggcgcgcaag ccccatatat aatttttctc gactgcaggc cagattaaga
      541 cgcagatagc cctctccttc agtaccataa acatgccccg ccataatacc tactctccct
      601 tcgttaatta atcgacggta aaatttatca aagggataac caagggccga gcaatcaacc
      661 cacgaaaaat agcagccctc agctggccat acgatcaatt ccggaatatt ttcatttaaa
      721 tactgcaagg taaattgaat gttagaggca atatgtacct ttaatgactt taaccattct
      781 tcacattcat tataggcaac aatggtcgca attacattca gtactgatgg ggagtttacg
      841 aaatcgcgat acctcgatat cgttctgaac ttatgattaa aattatcgtc agttgaaatc
      901 atatatgatc cggttagcgc aggaacatta aatgttttcg aaatcgaatt aaacaccacc
      961 actttgtcat cttttattgt tagcgctgac gtgaaactat gattaaaaac aaaatcacta
     1021 tgtatttcat ctgaacataa ccaaatatta tgccttgcac agcatgctgc gattctgtga
     1081 agatcgtctt ccgaccagac cgttcctgtc gggttatgag gattacataa taacaccatc
     1141 ttcgcgcttt taattttaag ctcaaagtct tgccagttga taacgtagcc tctcccattt
     1201 tctttcatca aagaggttgt tagcatgttt cgaccattat gcttaatcac atcaaaaaat
     1261 gcgttataaa caggcgtgaa aatgagaata ttatctccat tcaatgtcaa tatttctatt
     1321 gccttagata ttgaatacat cacgcttggg ctataggtga tccattctgg ttgaatctct
     1381 gttttatatt gttgatgata ccaattgact atcgaattca gatagtcatc atgctgccaa
     1441 cgactgtacc ctaaaatcgg gtgttccaga cgttttttta atgttctgat gatagcttcc
     1501 ggtatcggaa gatccatatc tgaaatagag aatggtaata catcttttac accaaatctg
     1561 tcttcacaaa agtcccattg tgtgcaatat gttcctttac gagaaattga ggtattaaaa
     1621 atatccatca acatatcccc atgtaattat ccatatcccg acgtagggcg atgacttttg
     1681 taccgataat gacttgtaag gtattttgat caagtttcac aaccgcaatt cctccggccc
     1741 tttttattgc agcctcattt atcaatccca tatccgcgat agttaaacgc aaacgagtta
     1801 cacaattatc cagtgattta atattttctt taccacccag agccttaacc atttgctccg
     1861 ctgcctgcaa ctctgattta tcatgaactg ggatgttagc tattgcagtt tcttcgtgag
     1921 cgttcagcgt gacaggactg gactcttcac ggcctacagt catcaggtta aatttgagaa
     1981 tggtccaacg gaacacaaag taataaatga agaaataagc cacttcaaca cataccgcta
     2041 atggccatcc cgttcttgtg ccacgtaaga caccaaagct aataaattga atcagatcgc
     2101 cagtaaagcc catcttcacg cccatatatg gcagaaccat gttagccaat ccattcataa
     2161 aggcatggaa gacaaacaac agcgggctga caaataagaa cagaaattca atcggctcgc
     2221 tgacgccgcc aataattacc gtgagcacac cggaaatcag caatgctttg atagcccttc
     2281 tgttttgagc atgagcagtg cgatacatcg ccagcgcggc tccagccaga ccatactgaa
     2341 tcatcagttt gccctgctcc attttccccg ccaaatccag cggaatgacc gcattctctt
     2401 tgacgtatgc cataaacatg ttcagggtgc cgtaatactc ttcaccgcca atcactgccg
     2461 atccgccgat tggggtaaag cggaaaactg atgtcaccaa gtgatttaag ccaaaaggta
     2521 tcgtcacgcg ttcggcaaca gcatagaaga aataaccaac aggaccggaa gttgaaatcc
     2581 agtgaccaat gagcatgaaa agattgaaaa atggcggcca gataaaagga atgatcagac
     2641 caaatccact catcacaatc agtgtaatga taggcacaag gcgtgggcca ctataaaacc
     2701 ctaattattc aggaatacgt aagttaacga tctttttata catgctggcg actaataacc
     2761 ctgccacaat cccacccaac acgctggtat tgtaggactg gatcccgagt atgatggttt
     2821 gcccatgtgt cgacatttgg tcagcagcta ccaataagtc gtgctgttta agataaaagt
     2881 tcgttccaag atgcatcgcc ataaagccaa ttaagccaga aaaagcgcca taggctttat
     2941 catctttctc ttttaataat cccaaaggta tagctatcgc aaacaataca ggtaaattaa
     3001 cgaaggcaaa caaaccaagg ctaacaataa aatcaagtat ggttttaatt aacggaatag
     3061 ccaacactgg aatgaatttt gccatatcat cgctggctaa accacttccc agccctaaca
     3121 tcataccaca tacacttaac agagcgatgg gatacataaa tgccttcccc aggctctgaa
     3181 aaaaactcca ggatttcttt tgtttcatac tggttatctc atgaaaatgt tatgtataat
     3241 tagtccatta atactttgat gcgaatagaa agatatagtt tctcttccag aatcaaatca
     3301 tatttaaaag ttgcgtacag atatctttca atttcatgaa tggcacgttg taactgaaaa
     3361 tctttttcaa gaaactgctc atagatatca ccaatatttt tctgttttat tttttcatta
     3421 cataacacac gactagaaaa atatttaagg tgcatcataa atctggagta gttaaatgaa
     3481 cttctgtcca cccttctgtt ataaacaact tcaataagct ccgtgatttt ttgagacagc
     3541 tcaataatcc ttactgattc ataatcaact gaattttcat aattatttaa aatatgcaat
     3601 actataaaac caatctcatc atccggtagt tgaacgctaa attgctcatt aatgattgtt
     3661 aatgcatcac gacttattgc atactctgct ttgtacaacg ctttgatttc ctcaaggaaa
     3721 acattattaa tttgtatgcc tgagacgact cgggatattg cattcgaaat gtgatcagca
     3781 agaatcagta gtagcgatgg attgagtttt tttcgagatt actctccgca taatgcacta
     3841 tcttttcggc aacaaaaata tactcaatac ccacatgctc aatcaatttg tagagtttat
     3901 ttttttgctc atttctgaca taaaaatccg gtttgcagga tgctcaggga ccgacatacc
     3961 atatttttat tgtaaccaac gccaggccca gaaatgataa cctcctgtcc gttcaccgat
     4021 gcctgtacac aattattgtt catgactttt tcgatgatca ttccatggct ccccaaaaaa
     4081 aaggcaaggc atgtcagaag taataattac ttcaaacatt gccttgcctg atttaacagt
     4141 tgcaagataa ttaactaatg cgtatacctt acacggaatt gtgcttaata aaagagatga
     4201 cagacttatt ttttaagccg atattcaaat attcgacagc agtcacaaaa ccatcgttca
     4261 acatttatgt aaataatatt tattcaatga aacataaatt cattgaataa attaaaaaca
     4321 tcatcccctt ctcgccagcg tcgccccatc ggcaaaatac gctttaatcc ccgcaagaat
     4381 agactccgca acttcctgct ggaaagtcgc cgttttcagt ttacgctctt cctcaacgtt
     4441 actaataaac gccgtttcga ccagaatgga gggaatatct ggtgccttta gtacggcaaa
     4501 cccggcctgt tcaacttgat ttttatgcag cttgttgatt ttaccgagct tattcagtac
     4561 cgctttacca aacttcaggc tgtcggcaat ggtcagcgac tgcaccatat cgaacatggt
     4621 gtggtcgaca tagcggtcac cgcttttgct cacgccacca atcaagtccg aggcgttctg
     4681 ggtttgtgcc agatattttg ccgcagtact ggttgcacct ttggttgaga gcgcaaacac
     4741 agaggaaccg ctcggctgac gactggtaaa ggcgtcggca tggatagaga caaacaggtc
     4801 agcacgctgt ttctgggctt ttgctacgcg cacttgcaac ggaatgaaga tgtcttcatt
     4861 gcgcgtcatg tacaccttca tattgccctc tttctcgatc agagagcgca gacggcgagc
     4921 tatttgcaat accacgtctt tttcgcgcgt tttgtatttc cccaccgcac cggagtcttc
     4981 gccgccgtgg ccagggtcaa gcataataac aataggacga tcgcgccctg ctttacccgg
     5041 ttgtggacca ctttgtgctg gcggcacctg cttttcgagg tcgcctttgt tgtaatcctc
     5101 cagcagcgcc agcagcgggt cctgcatatc ctgtgcattg gccggataga ggtccatcac
     5161 cagacgctct ttaaacccgg cgaccggcgc aagggcaaac agctgcggtt ttacgttttg
     5221 ctttaattca aaaaccatcc gtacggtctg cgggtcaaat tgcccgacgc gcgccgactt
     5281 gatgaacggg tcgtctgcac gaatttgtgc cgccatcccc ttgagtaccg agttcaggtt
     5341 tacatcttcg atatccacca ccacgcgttc agggttactc aacgcgaact gcttatattt
     5401 cagctgacga tttgattcta ccgtcacacg ggtgtagctg gacgcaggcc agacgcgcac
     5461 cgccacgacc tggctgaccg cagccagact gacctgactt acgctcaata gccacatggc
     5521 acccgcgcct tgcagtaaac gacgacggct gattgcagtg ttggatcctg acatgcctct
     5581 cccgagcaaa agaaatctaa gctgtgtaac aagtaaacga ctaatttgac cggaaacttt
     5641 agcgaaagac gcataatctg tcatctaata aacggtaaac attcttttta tattcacggc
     5701 attactgata aaaaagtcgc tctcgcataa aatttacact tgcaccctgc gaaaaaacag
     5761 aataaaaata cactaattta gaataatcat gcaaaggggt gtgccgtggt aaaggaacgt
     5821 aaaaccgagc tggtcgaggg attccgccat tcggttccct atatcaatac ccaccgggga
     5881 aaaacgtttg tcatcatgct cggcggtgaa gccattgagc atgagaattt ctccagtatc
     5941 gttaatgata tcgggttgtt gcacagcctc ggcatccgtc tggtggtggt ctatggcgca
     6001 cgtccgcaga tcgacgcaaa tctggccgcg catcaccacg aaccgctgta tcacaagaat
     6061 atacgtgtga ccgacgccaa aacactggaa ctggtgaagc aggctgcggg aacattgcaa
     6121 ctggacatca ctgctcgcct gtcgatgagt ctcaataaca cgccgctaca gggcgcgcat
     6181 atcaacgtcg tcagtggcaa ttttattatt gcccagccgc tgggcgtcga tgacggcgtg
     6241 gattactgcc atagcgggcg tatccggcgg attgatgaag acgcgatcca tcgtcaactg
     6301 gacagcggtg caatagtgct aatggggccg gtcgctgttt cagtcactgg cgagagcttt
     6361 aatctgacct cggaagagat tgccactcaa ctggccatca aactgaaagc tgagaagatg
     6421 attggttttt gctcttcaca gggcgtcact aatgacgacg gtgatattgt ctcagaactt
     6481 ttccctaacg aagcgcaagc gcgggtagaa gcccaggaag agaaaggcga ttacaactcc
     6541 ggtacggtgc gctttttgcg tggcgcagtg aaagcctgcc gcagcggcgt gcgtcgctgt
     6601 catttaatca gttatcagga agatggcgcg ctgttgcaag agttgttctc acgcgacggt
     6661 atcggtacgc agattgtgat ggaaagcgcc gagcagattc gtcgcgcaac aatcaacgat
     6721 attggcggta ttctggagtt gattcgcccg ctggagcagc aaggtattct ggtgcgccgt
     6781 tctcgcgagc agctggagat ggaaatcgac aaattcacca ttattcagcg cgataacacg
     6841 actattgcct gcgccgcgct ctatccgttc ccggaagaga agattgggga aatggcctgt
     6901 gtggcagttc acccggatta ccgcagttca tcacggggcg aggttctgct ggaacgcatt
     6961 gccgctcagg cgaagcagag cggcttaagc aaattgtttg tgctgaccac gcgcagtatt
     7021 cactggttcc aggaacgtgg atttaccccg gtggatattg atttactgcc cgagagcaaa
     7081 aagcagttgt acaactacca gcgtaaatcc aaagtgttga tggcggattt agggtaagcg
     7141 atgaaaattg tcggatgcga catgcgtaac actcgtgcat cgcatccggc aattacgttt
     7201 attcccgcga actaaacaac gccgccagac cactgcgccg ctcagtacga gtggcgattg
     7261 ccgcacttaa tatacgctca tcggcataca gcgacagacg gcgacgcgcg cgggtcaccg
     7321 cggtgtatac cagctctcgc gttactaccg gcgtgcgttg gctcggtaaa atcaacgccg
     7381 catggtcgaa ctccgatccc tgcgatttat gtaccgtcat cgcccacgta gtttcgtgct
     7441 ctggcaagcg actcggttgc acagacttaa tattgccgtc cggcatcgca aaccagacgc
     7501 gcgtcccctg cccgcgatcc agcgcaatac cgatatcgcc attaaacaac ccaagcgcgc
     7561 tgtcattacg ggcaatcatc accggtcgac cttcgtacca acgagagtgc ggatgacgat
     7621 gaattttgcg cttctgttgc ataaactgct caattcgctc attcagtcca gccacgccaa
     7681 acggcccttc ccgcagggcg cacaaaagtt ggtactcatt gaacgcctga atgattaaat
     7741 ccggctcggc acgcgcttgc agcagatcca gataacgtcc ataacccgca agagcttcct
     7801 caagcatcgc aatataatct tcgccgctct gtaaaagtcg tttttcgata tcagtaaaat
     7861 cctgctgaaa aacggttttc actgccgttt tatcaccacg gttgatcgcc gcagctaact
     7921 gaccaatgcc agaatcgctg ccgaaacgat agcttttttg cagcaggcag agactgtcgc
     7981 gcaaagatgc cgcttctgtg ccggttcctg ccggaacgtg agtccccgtc aggcggctta
     8041 gctgcctggc acgctcggcg gtaaagcccg cgttggcata agcgcagata tcgcccagca
     8101 cagccccagc ctcaaccgag gccagttgat cacgatcgcc gagaaagatc actcgcgcat
     8161 gatcgggcaa ggcgtcgatc agtctcgaca tcataggcag atcgatcatt gacgcttcat
     8221 ctaccaccag cacatcaaga tgcagcgggt taccggcatg atgacgtaaa cgctggctac
     8281 ccggctgcgc gcccagcaat cggtgcaaag tgctggcatc ttccggaatg cgtttctttt
     8341 gttcatcggt cagcggtaac tgtcgcaaag ccttgccgag agattcggtt aagcgcgcgg
     8401 cagctttacc cgttggtgca gccagacgga tacggcagcg ttcgccgtcg gccatttgaa
     8461 ttaacgctgc cagcaacttc gccacggtgg tcgttttacc ggtgcctggg ccgccggaaa
     8521 tcaccgagat ccgccgcgtc agcgtcactg ccgctgcaac tttttgccag ttaatttcat
     8581 cgcttactgg aaaaagtttg tccagggttt gcgccagtag agcttcatca acctcaatgg
     8641 catgattcac ctcattgaag aagcgcgcta ccgtccgctc gttacaccac atgcgattca
     8701 aataaagacg atcgccacag aggatcatcg gcgtgggttc atctcccctg ctgaccgctt
     8761 gagaagccag caagcattct tcccaatttt gtagctcacc gatttcactg acacaggtcg
     8821 ccaacagcgg atgcgacgcc tcgttatttt ccagtcgtga aagcggcaaa cagacgtgtc
     8881 cctctccggc atcatgactt aacagtgccg ccgcgagggt gacggcagga tgttcatctc
     8941 ccgccacggt cagggcaaat tgcacatcca gcgggcgtag ctgtttgtgc tccacagctt
     9001 ccagtaattg cttttgcaat ttcattacgc ctcctccagg gtcataccgg caaacatctc
     9061 atccatcagg gcaatcaacc cggcgttggg tcgggttgtg taaatccctt gttgcggatg
     9121 ttctttatca acgccacgca ggaacagata gataacgccg ccaaagtgac gatcatagtc
     9181 gtaatcagca atgcgatggc gcagataacg atgcagcgcc agggtataaa gctgatattg
     9241 cagatcatag cggtgtgcct gcatcgctgc tgccatagcc tgttgggtgt aagccgaact
     9301 gtcttcaccc agccagttgg atttatagtc gagcaggtaa taacgccctt cgtggcggaa
     9361 tactaggtcg ataaagcctt ttaacatgcc gcgtacctgc atgaactcca gcggcgggca
     9421 gccagcggat agcggatcaa actggcgaat tagtgcatca agctggctgg cgatgagcgg
     9481 ttcgctaatc ggcaggtaaa actccatctc cacctgttta tcgcggtcgg aaagctgact
     9541 caggctaacc cccttttcat tgagaggtgc ctggaggaca gccgtgatcc actcggtcaa
     9601 caccggttcc cactgcgatt caaagccgcc gagttccagt ttttcctgta cccagttcgg
     9661 gtcaaccggc tgggtaaaat cgaggtcttc aaacaaactg tgcaagaatg tccccggtga
     9721 cgcaccgcgc gggaactgat gcggtgttaa cgtcggttct tcaacgacgc tgaccacgcc
     9781 tgcggcatca acatccagcc gtggcatcag atcctgagcg ataccgtgac cacgctgctg
     9841 caagccggag tagctggtga cacgccagtt atcgccgggc aatcgttgta gtgtcctcgc
     9901 attcagttct gcagttaaag catcattaac ctgccagggc tggttatcac cagtttgtgc
     9961 cgtttgccag gcaatatcat catcgcataa cgcttcaata caggtgcgaa gccctgccgc
    10021 atcttgcggt tccccttttt gcagcaaacg cccgagcgca ctttggtgga cgtcggtgtc
    10081 acctttttta tcgccacgac ggcgcaccag cggtgcaacg ccgagactgc aatgccaaac
    10141 cgaacgcgtc agcgccacgt aaagcaaacg cagatcttcc gccagacgtt cgacctccgc
    10201 gaggtcgacg ctttttggcg cagcattaag atccaaaact gcctcaaacg agtggcgatc
    10261 gtgataaaac gcctgatcct ggacgcggaa attggtaata aatggcaacc agaccagcgg
    10321 atattccagc cctttcgatt tatggatcgt gacaatctgc accagatgtt tatcactttc
    10381 gaggcgcatt tgttggctgg aggcattact gtctggctcg aggatatgtt gcgataacca
    10441 gcgtaccagc gcatgttcac tttccagctg cgttccggct tcttgtagca gttcgctgat
    10501 atgcaagata tcggtaagac gccgctcacc gcctgccgtt gccagcaagt tttctgcaat
    10561 gttacgcgcc gacatcagcg cccgcagcat cggcataacg ccacgtttgc gccagatttg
    10621 ccgataacca tcgaactctt caactaccgc atcccacgca tgttcgtcat tattcagcgt
    10681 ttcgatatcc agcgcgttca gccccatcat tgacgttgcc agcgcactac gcagggtgtt
    10741 ctcacgttcg ggcgtcatca ccgcctgcaa taaccaaagc atttcctgcg cttccagagt
    10801 ttcaaaaaca ctgtcgcggt tcgaaaggta aacggaaggg atttccagca acgttaaggc
    10861 atcgcgcacc tgggcggcct cctggcggct gcgcaccagc acactgatgt ccgaagcacg
    10921 caccggacgc gcgtcgtcgc cgttcatcag caacgcttcg ccccgctgtc cggcttgtag
    10981 ccagtcgcgg atttgcgcag cacatacctg cgccatggta ctttgatagt cgccaacgcc
    11041 gcagctttcg ccttccatca gccacatttt catcgcaggc tgcgtttcac ctttaaatac
    11101 aaaacgtaac gcctgatttt tcccggctga tttcactgga ataaacggta tttcgcgaaa
    11161 catgaacgcg tcatcagtct ggctgaaaag cttgttcaca ctgttcacca ttccaggtgc
    11221 ggagcgccag ttggtgtcta aagtgtagtg ggcgtgaact tcactacgcg ccttcatata
    11281 agtgaagata tccgcacccc ggaatgcata tatggcctgc ttcgggtcgc caatcagcaa
    11341 caatgcggtt tccggctgat ggtgccagat acggcgaaaa attcggtact gctgggggtc
    11401 ggtatcctga aattcatcga tcattgccac cgggaatcgc gtacggatcg ccgctgccaa
    11461 cacctcaccg ctttcgctac gcagcgcgga atcgagccga cttaacatgt catcaaaacc
    11521 caattcgcca cggcggcgtt tttcacgcgc tactgtttcg cggatctcag ccaatgcgcg
    11581 ggtgatcacc agatcgcgga tcgacaatgg ttctgcaagc agttgatcga tcgcctcaaa
    11641 cagtggatgt cgcggggttt cccccccagc tttcgtgcga tcttctaaga aacgctggga
    11701 gaatttttcc agcgactccg gcaactgata actgttggtc tcttcttctg cccaggcgct
    11761 gatcttgtcg atccatttag cctgattgct acggttaaac ttgcgtcgat caataccaga
    11821 agattcgatc agcgcatcca gttcacccac tgcgtcgcgc cactgctgtt ttaccgtagc
    11881 aatacgcgcc acaatttgcg cgtggcggga agccagcgtt tcatcatcgg gcggcggtgc
    11941 tttgataacc ggcgcttcgc cttgcagata acgattaata tcgcgcagca acgcctgcgg
    12001 ccctttccag gtttcaaaga cgacctgggc tatttcacgc ggcagcgggt agcagtggcg
    12061 acgccagaaa tcggcgcagg cctggtaacg tagcagagac tcatcttcaa tcagctgctg
    12121 ctcaaacagc atgccggatt caaaggcatt caggttgagc atgcgctggc aaaaaccgtg
    12181 aatagtaaag actgccgctt catccatctg ccgttcggct aacaacaacc actgcgcggc
    12241 ttgcgcttta tcgtcgatct cttccagcag acgttcgtac agtggattgt cggtagtttc
    12301 acgcagacag gcgatgcgca actcatggat attgctacgg atacgaccac gcaattctgc
    12361 cgtggcagcc tcggtaaagg tgaccaccag cagttcttca acggtcagcg ggcggggaaa
    12421 ggcggcggaa ccgcctagtc caagtaacag gcgcaaatag agcgccgcaa tcgtaaaggt
    12481 tttgcctgtg ccggcagagg cttcaatcag gcgctcaccc tgtaagggca agcgcaaagg
    12541 atctagtgtc tcggcgacat cactcattct tttcactcat caggggcatt gtttgctgca
    12601 acgcgctgac gttctcccac actttccagc cttcagggtg tacatattcg gctttcccgt
    12661 tctggctgcc ggaaatctgc gacagaatag ccatgccttg cggctcgacc accgcctgat
    12721 ggaagaaatc agcaagtttt tgcggcgtca gcagttttat ctgggccacg attttatcac
    12781 gcgaatcgaa gcgcatattg ccgcgatcga aatctttact taacttcgat gcttcttcgc
    12841 cgagcgtttg cggtgcctgc agcatctggg taattaccgc ctgctggatt tgcgcaaact
    12901 catctggctt catcgctcgc aattttgcct ctgtggttgg gaaaaacgcc ttgtaacgct
    12961 cccacaagaa tgaaggctgt ttatcattgc tttgcaaaag gaagcccatg ccccactgac
    13021 gccccacgct cattggaaac gcaaacacgg catagcccaa ttgttcttcg gtacgcaact
    13081 gattgtagaa ccacggctgt acgatctgcc ccaacagaga gctataggct gagctggtgt
    13141 attcatcgta gccagtcggt acaaataccg ctgccagtgc ggagtcggtg ctgttaccgg
    13201 ctttttcaaa gatgacggat tgttttttat cgaccactac atctttgttt cgacaccact
    13261 ctgaaccatc agcgcccaac tgtttttgca catcgcgtgc ctgcgttgtt gcctgggcct
    13321 cggtcatgtt gccgataacc ataaactctg gtcgagcccc tgattttaag gcgtcgcgat
    13381 aggccagcac ctctttcaac gtaatggagg gcaaaatttt acgccgttca tctcgcgaga
    13441 agtacggcac ttgcgagagc atctgcgcgg gcataatcgc ctgctcaaac gctttaccct
    13501 tttctgcgga atccatcatc tggttatacc aagacttcgc ctgctcaagc tgatcttccg
    13561 tagcggtata gctaaagtac ccctcgagca atgcctggaa cagctgcggc agacgctggg
    13621 tgtaaccatt agcattaacc ataaggccgt tgttagcgtt ggtggaaaaa cttatgccac
    13681 caaccgacgc ctggttgctt aactgatcaa gcgccagccc tgcgagataa tcattgagcg
    13741 caaacatcac ctgattgcgg gcgctgtcca tggctttcgg attacgcaaa atcaggctga
    13801 catcagcttt gggttcgctg gaaaaataac ggcttggcgc atacaccacg cgcagattcg
    13861 actcatcaac aatcagctct ggatggtcat atttcttctc tgacttaatc agcgagaaat
    13921 cgtcaggaat ataggggtta agctccggta aggagagcgc aatattggca gctttttgct
    13981 gccagtcagc gaaagtttgt tcgctgattt tatcgacctg atacggcgca tcgacaaaat
    14041 aagccgtttt attgtgcggc tctttcgggc tgatatacca gatacgcgca ttctgcggcg
    14101 tcatcatcgc cagacgttct tttactgctt tagcatcgta ccgatcggca atattgactg
    14161 catccagcgt atgctcaacc ggaacgcgaa tcatggtatc tgccagccat tcgacgtaat
    14221 ccatatcacg ggtgatcgac ggataacgga agtcgatatc cagcacattc gccagttcat
    14281 cgaagtattg tttatcaatg cctttttcac gtaacagatt gagatagcta aaaattgccg
    14341 ccacaacctg atcgcgatta gccaggcctt tatcggttaa agacgcagag atcgctaata
    14401 cgccgctgtt gccgttgacg ataggatcgg agttggcgct aatgccctca actaatccct
    14461 gcttttgcag ccagtcggaa agtgtacctg ggctgcgatt accaatcaga taggtaatca
    14521 attcatccgt tttactacgg aacttcgctg agttgttatc gatgcgaaac tcaacgcgca
    14581 gaactttacg cggcagtgcc gggacgtaat gaatgataat gcccttttgc gcgtcggtga
    14641 ctaccggcac ggtgatttcc ggttttttgc tctctttgtt cggcacgcga ccaaaggtgt
    14701 ccgccgccat ttttgccaac tccggcagcg gtttattgct gtaaataacc gccttcatca
    14761 gattggcgga atagtacttc tcgtggaaat ctttcagcgc ctgctgcacc ggattaccag
    14821 gtttatcgct taaagtttcg aggttaccac cagaaaactt tgaaccgggg tgtgccgggt
    14881 taatggtttc tgcgctgacc tgtgccatgc gcatcccgtc acgcgtacgc gccatggtta
    14941 attcagcgtt caccgcatta cgctcacgtt cggcatattt cttgtcgagc aaaggttcag
    15001 caatagcatc ggccaggcgg tctaccgcac caggcaaggc gtcgttctca acttccagat
    15061 agaaagccgt gcgatacggc gcagtgctgg cattgtgact accgccgtgc attttgagat
    15121 attcggccag actgtcagcc tgcgggtact ttttcgaccc catcagactc atatgttcaa
    15181 ggtaatgtgc cagcccctgg tacgcctcgg gatcttccag cgacccaacg ggcaccacca
    15241 gcgccgagag cgatttaact gcctgcggat cagaaaccag caagaccacc ataccgttat
    15301 ccagacgtat agcctgatac tggcggttat ctttatcact tttacggatg gtttcctgaa
    15361 tcggctgcca tcccgtttct gcctgactta agggtgccca aagggcaact aacaacaata
    15421 atgctttgaa ccaagtgctg cggggcattc acggacctca taatcaactt aattttctgt
    15481 ccagattcaa cacgttaacg caaatcatct gccagaattt aatcttgtgc tgcacgagtc
    15541 agcctatgtt tatataacca tcagtccgtg actggtgcgc atcataaagt aagcggatag
    15601 attgcgcaat ttttatacag cactcatgac tgattaaagc gaaacagcgg taacaggaaa
    15661 cgttgcgact gttcaacgat ggcctccatt gtctctggtg ttaattgccg ccagagcctt
    15721 tgataccaga tattatcacc ttcaccacgc accatcatgt tgccttcgta agcctgaagg
    15781 aatttcgtac gggctttttg caacgtggaa tcgtcatcca gcatggcatc gttttgcgcg
    15841 tcataacagg tttttagcca cgcgccgcca ctttcaggta acaccagcaa tggcgcggac
    15901 atcccttcac gatacccctc aatcagttgt gagaggtaat gcaaagcctg ttcggctgca
    15961 agcggcggaa aacgccactc gccgtctttg cgtagaaaaa ggcgactttc accattacca
    16021 ccgctggcac agtagacaag gtgttccagc caaagttgca ttccctgcgc cacacttaat
    16081 aaagagggac gccagcgcaa caggccatcc ggctgcacct gcggcaacca gccagttatc
    16141 tgcacaccgt tgcaggcgag atcaatttcc atactctgcc ccggctggcg acaggcaatg
    16201 actctgtcgg caagctgctg catctcctgg cactgagttt cccagaaaat ttcaccaaaa
    16261 gcgccatacg gtaaatcccc tgccgcccgg aagcgacgga acaagcgttc ggcatcatcc
    16321 tgctcaacca gtgcattcaa taactgctga ttgatttgat aacggctaag cccttccaga
    16381 ataaatggct cggtgtcggg gatttcgctg tcttcggtac ggaagttcac ctgcaaacgc
    16441 atctggaaaa atgcccgcac cggatgtgcc cagaatcgtt gtagcgtttc cagcggcacg
    16501 gtttccggta aggtaaacgg cagcggctga acaaattcag aatgtgcttt accagcctgg
    16561 ctggccgcag gtagccattc acgagcatag ctttgtcgtt cgcctggctg gtagttttgt
    16621 ggatcaaacg gcatccgggt atggaggcag gtaagatgcg cttttaccct tgcctcgctt
    16681 tcatcacagt tgagcgcttc atcgcccggt agataatgac tttgcccgat gtagtcgatc
    16741 agttcctgca ccagtaccga cgggaaacgt tcactgttat cctgaatgga aagcccgata
    16801 taactgatat agagtttttg ctgcgcggaa attaacgctt ccaggaacag gtagcggtcg
    16861 tcatcgcgac ggctacggtc gccacgcttc ggtttctggc tcatcaggtc aaagcccaat
    16921 ggcgcaagct gacgtggata aacgccgtcg ttcattccca gcaggcaaac cactttgaac
    16981 ggaattgaac gcattggcat cagagtacaa atgttaaccg gtccggcgag aaaacgctgg
    17041 ctgatacgtt cttgatccag acgctgtgcc agttcatcac gcaatagtga cagcggcacc
    17101 gcgtcgccat actgcgcacc taaaccttcg gcgataatcg cctgccattg ttgttcgatc
    17161 agcgtcatcg ccgcttcggt ttccgcatcc ggcaggaaga aggcgttgag catatcgcga
    17221 caaaccggca accactcttc tagcggacgc tcctgtgcca gcccgcgacg ccagatgttt
    17281 agctgcatta gcagtgaagc cagatgcccc accagttctg caattaagcc gctcgactca
    17341 tcataaggta gaaccgattg ccactcgccc tgcgcgctct ccatcgcgta gcccagcaac
    17401 atacgcgtca ggccaaatcg ccaggtgtgt tgtccggtgg cggggagttc cagctcgcga
    17461 acgttgtcgt catctatccc ccaacgaatg ccggattcgt tgacccacag gcgtaaataa
    17521 cgcagccctt cttcggtgat gtcaaaccgc gccgccagca ccggcacatc cagtaatgcc
    17581 agcacgtctt ccgacacaaa gcggctgtct ggcaacgata acaggctgat aaacgcttcc
    17641 agtaccggat gcgactgccg cgcccgacgg tcggaaatgg cgtaaggcag gtaacgatcc
    17701 gcaggtgcgc taccaaacac agcctgaata aacggactgt agctgtcgat atcagccacc
    17761 atcacgatga tgtcgcgcgg agtaagtgtc gggtcttctt ccagcatcgc cagcaggcga
    17821 tcgtgcaaaa cttcaacttc acgctgcggg ctatggcaaa cgtggaaggt gatactgcta
    17881 tccagtggat caagcgggcg tttgttatcg ctacgggaaa actcttcgat gttcacacca
    17941 gcaacggcac ggttttccag ttccagaatg tcagactgaa tgttatgcag caggttatct
    18001 ggcgtgacat cgacaaaggc gtccagctcc tggctgctct ccaggtcaga aaggagataa
    18061 atgtagtcgc gcccaagctt cccccatgag gccagcagcg ggttgccgac atcctgttca
    18121 ccatcgctgt taaagagctg tccggcattt tcgctatcgc gaaatagcgg caattcgcga
    18181 tcttcaaaac tgtgtcgacg ctgacgagtc agcagtttcg ccagataagc aggatcttta
    18241 atatcgcccc agtaataacg gcaggggttg gtaaacagga gatggatttc aatatgttta
    18301 cccagcgcct ggagcgcctg gagataaaca ggcggtaacg cggaaatacc gcatataaag
    18361 acgcgcgaag gtaaccccgg cgggcaggtc gtcgcggact ccagcgtttc gataaagcgc
    18421 tgatagagat tcgcgcggtg ccagcgcggt tgtccgagtt catgggtata ttccaccagc
    18481 gccttccaca acggcgcttg ccaggcctgt gcttctccca gcccttcaac caaatgtcct
    18541 gtttcccatt gtgccagcca ctccgggcga tagaccagat actggtcaaa caggtccgcc
    18601 gcttttgagg aaagctggaa cagttttcgc ttgtcgctat cgtcagtcag ataatgccgc
    18661 aacagggtaa agtcttcgtg ctccaacagt tgcggcagca gagtcatcag tttccagctc
    18721 atgctctgtt tgttaaaggc gctctctttg gggatctccg gtaacacccg gacgaacata
    18781 tcccagataa agctcgcagg cagcggaaaa tcaatgtttg ccgcaatacc aaacttttgc
    18841 gacagggtca tttgcagcca ctgtgccata ccggtacttt gcaccagaat catctctggt
    18901 tcgaaaggat cgtccagccg ttcgcgttcg acaataaact ccatcaacgc ttccagcacg
    18961 tccagacgat tggaatggta gacccttaac atagcggctc ctgactactg acgattcggg
    19021 caatgcaggc gggtcatctc gccttctctg cccccgggtg aaactagcgt aacgctgatg
    19081 ctgacacatc ccgcctgcga tgtctgcaat cggttgacct gccagttagc tggtggcgaa
    19141 atcgcgcgca gttgcgtttg ctgccagcca tgccgccaga gctgttggta ctggtttctg
    19201 ctggcaagac tgttcattaa tgttcgctgg aaacccgata acgcagtgac aatcatcacc
    19261 atcaacacca tcgccaacat tacctccggc aggctaaagc cttgttgatt cttcagggaa
    19321 gctgacataa cgccctctct ttcagcggac aaaaatcgct ccagccgctt ggcgaaaaaa
    19381 caatgtttcc atcaatgact tcgcctgttc gccacaacga aacgccttca taaccggcaa
    19441 tcaataaggc ttcattatct gccagtaaac gcaaacaaac ctgggcatcg gtttcagcgt
    19501 actgcgagca ctgaactgct ggctgcgtct gccaggagtg catttttccc cacgccagcg
    19561 ccgactgaac gatggcctgg cggcgcaatg actgactttc catgctcacg cgagaggcaa
    19621 aactgcgatc ctgttggctc attccttgta atagcaaact acccaaaacc agcagcatca
    19681 ggaccagagc cagtgacgaa acaccctttt cgcggttcac aggttgaatc ctgtcacgct
    19741 atagctggca ttcaccacgg tttgcggttc agacttactg gcagcacgca tattaaccgt
    19801 caacaccggc gagaagccgc tgacatcctg acgtacgacc tgaaaagtgt cgataatgat
    19861 ggcatccgga ttagtgactt tatcccagcc cttaccttca caggatgtcg caccgcgtag
    19921 cgtttccagc acatgctcct tcagacgaaa tccaatctgg tcggactctt ttaccggttc
    19981 gcgatcccag ataccgttac tgttcgcatc ccactgcaca atgataca
//
LOCUS       DAJVUJ010000069        19875 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.069, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000069 DAJVUJ010000000
VERSION     DAJVUJ010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19875
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.069"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            54..830
                     /gene="eutC"
                     /locus_tag="OQQ48_002216"
     CDS             54..830
                     /gene="eutC"
                     /locus_tag="OQQ48_002216"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HCS1952975.1"
                     /translation="MTSESCALDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTG
                     RAGPRPRTQALLRFLADHSRSKDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDM
                     GRRLCAEAVEALKAQCVANPDVQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVG
                     TPFFVRYGRVKIEDQIGEILGAKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEAD
                     RTCISNIHQGGTPPVEAAAVIVDLAKRMLEQKASGINMTR"
     gene            840..1499
                     /gene="eutL"
                     /locus_tag="OQQ48_002217"
     CDS             840..1499
                     /gene="eutL"
                     /locus_tag="OQQ48_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HCS1952976.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            1512..2012
                     /gene="eutK"
                     /locus_tag="OQQ48_002218"
     CDS             1512..2012
                     /gene="eutK"
                     /locus_tag="OQQ48_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HCS1952977.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAAGRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVREPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            2058..3110
                     /gene="eutR"
                     /locus_tag="OQQ48_002219"
     CDS             2058..3110
                     /gene="eutR"
                     /locus_tag="OQQ48_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HCS1952978.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEDAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFVTDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(3116..4015)
                     /gene="hemF"
                     /locus_tag="OQQ48_002220"
     CDS             complement(3116..4015)
                     /gene="hemF"
                     /locus_tag="OQQ48_002220"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HCS1952979.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(4019..4888)
                     /gene="amiA"
                     /locus_tag="OQQ48_002221"
     CDS             complement(4019..4888)
                     /gene="amiA"
                     /locus_tag="OQQ48_002221"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HCS1952980.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            5102..5527
                     /locus_tag="OQQ48_002222"
     CDS             5102..5527
                     /locus_tag="OQQ48_002222"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HCS1952981.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADMLSLGKRLIEDEEY"
     gene            5514..5963
                     /locus_tag="OQQ48_002223"
     CDS             5514..5963
                     /locus_tag="OQQ48_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HCS1952982.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFIIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            6170..6745
                     /locus_tag="OQQ48_002224"
     CDS             6170..6745
                     /locus_tag="OQQ48_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HCS1952983.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFRGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            6841..7740
                     /gene="yfeX"
                     /locus_tag="OQQ48_002225"
     CDS             6841..7740
                     /gene="yfeX"
                     /locus_tag="OQQ48_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HCS1952984.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(7798..9102)
                     /gene="pbp4b"
                     /locus_tag="OQQ48_002226"
                     /pseudo
     CDS             complement(7798..9102)
                     /gene="pbp4b"
                     /locus_tag="OQQ48_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
     gene            complement(9107..10531)
                     /gene="murP"
                     /locus_tag="OQQ48_002227"
     CDS             complement(9107..10531)
                     /gene="murP"
                     /locus_tag="OQQ48_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HCS1952985.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKSVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRSQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(10535..11431)
                     /gene="murQ"
                     /locus_tag="OQQ48_002228"
     CDS             complement(10535..11431)
                     /gene="murQ"
                     /locus_tag="OQQ48_002228"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCS1952986.1"
                     /translation="MQLEKMITEGSNAASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            11595..12452
                     /gene="murR"
                     /locus_tag="OQQ48_002229"
     CDS             11595..12452
                     /gene="murR"
                     /locus_tag="OQQ48_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HCS1952987.1"
                     /translation="MLYLTKISNAGSEFTENEQKIADFLQANVSELQSVSSRQMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNTTALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCVEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVASLKMIQR
                     SSELTQRLK"
     gene            12581..13372
                     /gene="ucpA"
                     /locus_tag="OQQ48_002230"
     CDS             12581..13372
                     /gene="ucpA"
                     /locus_tag="OQQ48_002230"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HCS1952988.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPM
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            13676..14692
                     /gene="cysP"
                     /locus_tag="OQQ48_002231"
     CDS             13676..14692
                     /gene="cysP"
                     /locus_tag="OQQ48_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HCS1952989.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKTDVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            14692..15525
                     /gene="cysT"
                     /locus_tag="OQQ48_002232"
     CDS             14692..15525
                     /gene="cysT"
                     /locus_tag="OQQ48_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HCS1952990.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            15525..16400
                     /gene="cysW"
                     /locus_tag="OQQ48_002233"
     CDS             15525..16400
                     /gene="cysW"
                     /locus_tag="OQQ48_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HCS1952991.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            16390..17487
                     /gene="cysA"
                     /locus_tag="OQQ48_002234"
     CDS             16390..17487
                     /gene="cysA"
                     /locus_tag="OQQ48_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HCS1952992.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPDQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGQVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            17621..18532
                     /gene="cysM"
                     /locus_tag="OQQ48_002235"
     CDS             17621..18532
                     /gene="cysM"
                     /locus_tag="OQQ48_002235"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HCS1952993.1"
                     /translation="MSTLEQTIGNTPLIKLQRMGPNNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(18535..18903)
                     /locus_tag="OQQ48_002236"
     CDS             complement(18535..18903)
                     /locus_tag="OQQ48_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HCS1952994.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTAHEEARINAMLEGLSQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            19008..19859
                     /gene="pdxK"
                     /locus_tag="OQQ48_002237"
                     /gene_synonym="thiJ"
     CDS             19008..19859
                     /gene="pdxK"
                     /locus_tag="OQQ48_002237"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HCS1952995.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
BASE COUNT         4575 a         5043 c         5608 g         4649 t
ORIGIN      
        1 cggcccccgc gctgtcagaa gcaaagtgca ccaccaccac ctgtgcggca ccggtgacct
       61 cggaaagctg cgcgctggat ttaggttctg cggaagcaaa agcgtggatt ggtgttgaaa
      121 acccgcatcg cgcagacgta ttaacagaac tgcgtcgcag caccgtggcc cgcgtttgta
      181 ccggtcgtgc cggtccgcgt ccgcgtaccc aggcgttgct gcgtttcctg gccgatcact
      241 ctcgttcgaa agataccgta ctgaaagaag tgccggaaga gtgggtgaaa gcgcagggct
      301 tgctggaagt acgctccgaa attagcgaca aaaatctcta cctgactcgc ccggatatgg
      361 gccgccgcct gtgtgcagaa gctgttgaag cgctgaaagc acagtgtgtt gctaatccgg
      421 acgtacaggt cgttatttct gatggcctgt caactgatgc catcaccgtg aactacgaag
      481 agatcctgcc gccgctgatg gcgggcctga aacaggccgg gctgaaagtc ggcacgccat
      541 tctttgttcg ttatggtcgc gtgaagattg aagatcagat cggtgagatc ctcggcgcga
      601 aagtggtgat cctgctggtg ggggaacgtc cggggttggg tcagtcagaa agcctctcct
      661 gttacgccgt ttactcgccg cgtatggcga ccaccgttga ggccgatcgc acctgcatct
      721 ctaacattca ccagggcggc acgccgccgg ttgaagccgc agccgtcatt gtggatttgg
      781 cgaaacgtat gctggagcag aaagcatccg gcatcaacat gacccgataa ggaggcatca
      841 tgccagcttt agatttgatt cgaccgtcgg taaccgccat gcgggtgatt gcctccgtta
      901 acgctgaatt tgcgcgtgaa ctaaaattgc cgccgcatat tcgtagcctc gggctgattt
      961 ctgctgattc cgatgacgtc acctacattg cggctgacga agcgaccaag caggcgatgg
     1021 tcgaagtggt atatggccgc tcgctgtatg cgggagcagc acacggaccg tcaccgactg
     1081 ccggtgaagt gctgattatg cttggtgggc caaacccggc ggaagtgcgt gctggtctgg
     1141 atgcgatggt tgcgcatatt gaaaatggcg ctgctttcca gtgggctaat gacgcagaaa
     1201 acaccgcatt cctggcacat gtagtttcgc gtaccggttc ttatctctca tcaaccgccg
     1261 ggatcacgct tggcgatccg atggcgtatc tggtggcacc gccgctggaa gcgacctacg
     1321 gcattgatgc agcgttgaaa tctgccgacg tacagctggt gacctatgtc ccgccaccgt
     1381 ctgaaaccaa ctactcggca gcatttttaa ccggtagcca ggccgcgtgt aaagcagcct
     1441 gtaacgcctt taccgatgca gtgctggaaa tcgcgcgtaa tccaatccag cgtgcgtaac
     1501 ggaggttgcc gatgatcaat gcactgggat tgctggaagt ggacggcatg gtcgccgcga
     1561 tagatgcagc ggatgccatg ctcaaagcag ctaacgttcg tctgctcagt cacgaagtgc
     1621 ttgaccctgg tcgcttaacg ctggtggtgg aaggcgatct ggcggcgggt cgtgcggcgc
     1681 tggacgcagg ttgtgctgcc gcgatgcgta ccgggcgtgt catcagccgc aaggagatcg
     1741 gtcggccaga cgatgacacc cagtggctgg ttactggctt taaccgccag ccgaagcaac
     1801 ccgtaaggga acccgacgcg ccagttatcg tcgcggaatc tgctgacgag ttgttggcgc
     1861 tgttaacatc agtacgtcag ggaatgacgg caggagaagt ggctgcccac tttggctggc
     1921 cgctggaaaa agccagaaat gcgctcgaac agctcttttc tgccgggacg ttacgtaaac
     1981 gcagtagtcg ttatcgtctc aagccccatt aacctgtcgg aggtgctggg tgtcctacaa
     2041 cacccggcat taacatcatg aaaaagaccc gtacagccaa tttgcaccat ctttatcatg
     2101 aacccttacc cgaaaacctg aagctcacgc cgaaggtcga agtggataat gttcatcaac
     2161 gacagacaac ggatgtctat gaacatgctt taacgattac cgcctggcag cagatttacg
     2221 atcagctgca tccgggcaag tttcatggtg aatttacgga aattctactc gatgatattc
     2281 aggtttttcg tgaatacacc ggtctggcgc tgcgtcagtc gtgcctggtc tggccgaact
     2341 cgttctggtt tggcattccg gcgacgcgcg gtgagcaggg atttatcggt tcgcaatgtc
     2401 tgggaagcgc ggaaatcgcc acccgccctg gtggcactga atttgaactg agcacgccgg
     2461 atgattacac gatcctgggc gtggtgcttt ctgaagatgt catcacccgg caggctaact
     2521 ttttgcataa cccggatcgg gtattacata tgctgcgtag ccagtcggcg ctggaagtga
     2581 aagagcagca taaagccgcg ctgtggggct ttgtccaaca ggcgctggcg acgttttgcg
     2641 agaacccgga aaatctccat cagcctgcag tgcgaaaagt gctgggggat aatttgctaa
     2701 tggcgatggg ggcgatgctg gaagacgcgc aaccaatggt gacggcggaa agcatcagtc
     2761 atcagagtta ccgtagatta ctttcccgcg cccgtgaata tgtgctggaa aatatgtctg
     2821 agccggtgac ggtgctggac ttgtgtaatc aactgcatgt cagtcgccgc acgctacaaa
     2881 acgcgtttca cgctatttta ggcattggcc caaacgcgtg gctgaaacgc attcgcctga
     2941 acgccgtacg ccgcgaactg ataagcccgt ggtcgcaaag cacaacggta aaagacgccg
     3001 ccatgcagtg gggattctgg catctggggc aatttgtcac ggattaccag cagctgtttg
     3061 ccgagaagcc gtcgttgacg ttgcatcagc ggatgcggga atgggggtga gggagttaca
     3121 cccaatccct gaccttaata aactcactta acgccgcttc tgggctgcca tcttttggct
     3181 gataatcata ctcccagcgt accagtggcg gcattgacat caggatcgac tcggtgcgcc
     3241 cgccagtttg caggccaaac agagtgccgc gatcccagac cagattgaac tcgacataac
     3301 gaccgcgacg gtagagctga aaattgcgct cgcgctcgcc gtaggccatt gctttacgtc
     3361 gctctacaat tggtaaataa gcgtcggtgt agcctttgcc taccgcctgc ataaaggcaa
     3421 aacagtggtc gaaatctggc gtgttcagat catcaaagaa cagcccgcca ataccgcgct
     3481 gttcgttgcg atgtttgagg tagaagtaat cgtcgcacca ctttttgtaa cggggataaa
     3541 cgtcttcacc aaatggcagg cacaggtcac gggcggtgcg gtgccagtga atggcgtctt
     3601 cttcaaaacc atagaaaggg gttaaatcga agccgccgcc aaaccaccag acgggctctg
     3661 cacccggttt ttcggcaata aaaaaacgca cattcgcgtg gctggtggga acatacgggt
     3721 tatgcggatg cactaccagt gaaacgccca tcgcctcgaa actgcgcccg gcaagttccg
     3781 ggcgatgagc ggtggcggaa gcaggcatcg cctcgccgtg gacgtgagaa aagttgacgc
     3841 ctgcctgttc gaaaacacca ccattacgca acacccgact acgcccgccg ccgccagctt
     3901 cgcgctgcca actatcttcg acaaattctg cgccatcgac ggcggtcagc tgctgacaaa
     3961 tcgtatcctg aaggttgagc agaaactgtt taacctggtg tgcgtcgggt ttcataactt
     4021 atcgcttttt cgaatgtgct ttctggttgt cgaaccagtg gaaataactg atcacgcctt
     4081 cagcaatcgc tgtggcgatt ttctgacgaa acgccgccgt gcctaacagc cgctcttctt
     4141 ccgggttggt gataaacgag gtttccacca gcaccgaagg aaccgacggc gatttcaaca
     4201 ccacaaacgc ggcttgttcg gtgttgcggc tgtgcagttt atgcaccggc ttaatctttt
     4261 tcagaatatg cgagccaagc gtcaggctgt ttttaatagt atcggtttgc accagatcaa
     4321 acagcacttg ctgcaatagg tgatccttgt cagtcgcctt tttaccggca acttcatcgg
     4381 cgcggttttc gcgttcagac aggtatttcg ccattgcgct acttgcccca cggttagaga
     4441 gggcaaatac cgaagcaccg gcagctttcg ggttggtaaa gccatcggca tgaattgaca
     4501 taaacagatc tgcgccatgt ttatgggcga tttcaacgcg atcgtaaagt gggataaacg
     4561 tatcgccaga acgcgttaaa cgcgcatcaa tcccatgatt acgcaaaatg gaacggacgt
     4621 ttttagcaat cgccagcacc acatgttttt ctttcgaacc gttgcgtccg atcgctccgg
     4681 tatcaattcc gccgtggcct ggatcgagaa caacgacacg tttgccgcca gattttttgg
     4741 ctttcggctt gctgtgtccg ttgctggttt ttaaaggttc gtctttggcg atggcttgcg
     4801 acattcctga caacgtcagg gcagccaaac cggctttcag cacctggcgg cgcgaagtga
     4861 gtgtttttag tggtttaaaa gtgctcatac ggcctgagtt gtaataataa agttccagat
     4921 gttatatcgt atcgcgtaat ccgttacgac ggtttgatgt tgagaattgt tttacttttc
     4981 atttcaatag ttgacaactc cccattatgc cactttttca accgattttc gtcagatatt
     5041 ggctaattct accgttcacg ccataatcag ccttttaacc cgcaaaaagg taacgaatac
     5101 catggagata cgcgtatttc gccaggaaga tttcgaagag gtcatcaccc tttgggagcg
     5161 ttgcgacttg ctgcgtccgt ggaacgatcc ggaaatggac atcgagcgta agatgaacca
     5221 tgacgtcagt ttgtttttgg tcgctgaggt gaacggtgaa gtggtcggaa cggtgatggg
     5281 cggttatgac gggcatcgcg ggtctgctta ttatcttggc gtgcatccag agtttcgtgg
     5341 gcgtgggatt gccaatgcgt tgcttaatcg gctggagaaa aagctgattg ctcgtggctg
     5401 cccgaaaatt cagatcaatg tgccggaaga taacgacatg gtgctcggaa tgtatgaacg
     5461 cctgggatat gaacacgccg acatgctgag tctgggtaag cgtttgattg aagatgaaga
     5521 gtactgagtt ccatcctgtc cattatgatg cgcacggacg cctgcgttta cccttgctct
     5581 tctggcttgt gctattgctt caggcgcgaa cctgggtgct gtttatcatt gccggtgcgt
     5641 cgcgcgagca gggtactgcg ctgctaaatc tgttttatcc cgatcacgat aatttctggc
     5701 tggggttgat tcccggtatt ccggcggtgc tggcgttttt gttgagcggt cggcgggctt
     5761 cgtttcctcg cacctggcat gtgctctatt ttctgttgct gttggcgcag gtggttttac
     5821 tttgctggca accgtggctg tggctgaacg gtgaatccgt tagcggtatc ggtctggcgc
     5881 tggtagtggc ggatattgtg gcgttaatct ggctgctgac caatcgacgt ttacgcgctt
     5941 gttttaatga agaaaaagaa taacgcagag gtgcgctttg tttatgccgg atgcggcgtg
     6001 aacgccttat ccggcctaca aaacatcgaa aattcaatat attgcaggag aaagtaggcc
     6061 tgataagcgt agcgcatcag gcaattttgt gtttgtcatc aacctttaac ggcacttttt
     6121 ggtgaatttg cactccaagc aacgttattg aataaccaaa ggaagtgaca tgaaatcgct
     6181 gcgtttaatg ttatgcgcta tgccgttgat gctgaccggc tgttccacga tgtcgtcagt
     6241 taactggtcg gctgctaacc cgtggaactg gtttggatcg tccaccaaag tgagcgagca
     6301 gggtgtgggt gaattaacgg cgtccacacc actgcaagaa caagccattg ctgatgcgct
     6361 tgatggcgat tatcgcctgc gcagcggaat gaaaaccgcg aacggcaacg tggtgcgctt
     6421 ttttgaagtg atgaaaggcg acaacgtggc gatggtgatt aacggcgatc aaggcacgat
     6481 cagtcgcatt gacgtgctgg atagcgatat tcctgctgac accggtgtta aaatcggtac
     6541 accgtttagc gacctttaca gcaaagcatt tggcaattgc caaaaagctg atggtgatga
     6601 taatcgtgcc gtcgaatgta aagccgaagg cagtcaacat attagctacc agttcagagg
     6661 ggaatggcgt ggtcctgaag ggttaatgcc ttcggacgat accctgaaaa actggaaagt
     6721 cagtaaaatt atctggcggc gttaatttgc gtctgaacaa accgccgccg cgaaaaaaca
     6781 cgtaaaatag cgctcaacaa tgccacggat tgcgtggcat tcttattttc aggaggaaca
     6841 atgtctcagg ttcagagtgg cattttgcca gaacattgcc gcgcggcgat ttggatcgaa
     6901 gccaacgtga aaggggaagt tgacgccctg cgtgcggcca gtaaaacatt tgccgacaaa
     6961 ctggcaactt ttgaagcgaa attcccggac gcgcatcttg gtgcggtggt tgcctttggt
     7021 aacaacacct ggcgcgctct gagcggcggc gttggggcag aagagctgaa agattttccg
     7081 ggctacggta aaggccttgc gccgacgacc cagttcgatg tgttgatcca cattctttct
     7141 ctgcgtcacg acgtaaactt ctctgtcgct caggcggcga tggaagcctt tggtgactgc
     7201 attgaagtga aagaagagat ccacggcttc cgttgggtgg aagagcgtga cctgagcggc
     7261 tttgttgacg gtacagaaaa cccggcgggt gaagagacgc gccgcgaagt ggcggttatc
     7321 aaagacggcg tggatgccgg cggcagctat gtgtttgtgc agcgttggga gcacaacctg
     7381 aagcagctca accggatgag cgttcacgat caggagatga tgatcgggcg caccaaagag
     7441 gccaacgaag agattgacgg cgacgaacgt ccggaaacct ctcacctcac ccgcgttgat
     7501 ctgaaagaag atggcaaagg gctgaagatt gttcgccaga gcctgccgta cggcaccgcc
     7561 agtggcactc acggtctgta cttctgcgcc tactgcgcgc gtctgcataa cattgagcag
     7621 caactgctga gcatgtttgg cgataccgat ggtaagcgtg atgcgatgtt gcgcttcacc
     7681 aaaccggtaa ccggcggcta ttacttcgcg ccgtcgctgg acaagctgat ggcgctgtaa
     7741 gcgtttctga ttctatgatt aaaggccagc cagtaacaaa actgactggc ctttttatta
     7801 cttttgcttc aacgccgcat acacctgatc aactacccaa ccataagttg caatcggtaa
     7861 ctgaccgctt tcgaacatat tgggattttt ttgtggatcg gcaactggcg aatggggctt
     7921 gttgcttaac atcacaatcg ccatatgatt gaccggatcg ataacggtta ctgtcccggt
     7981 ccagccagtg tgaccgtagg tttgcgggct tgccagcgtg ccaaacgtcg gcgtcatggt
     8041 ggcattacca ttcacgcgcc agccgaggcc aaaagtggca tcttccttag agctggtggt
     8101 gaacatcttc accgtttccg cactgaacag ctgcacatcg ccatagcccc cgccgttcag
     8161 catcgtttgc attaacaccg caatatcgcc ggtattggaa aacaaacctg cgtgcccgga
     8221 aacgccgccc atcgaataaa aggctttttc atcgtgcacc tgaccccaga gagtggaggt
     8281 gcggatattc gggaaatgga tcacgccatc gcgggtattg ccgttcaatt ccgtggcggc
     8341 aatttgttgc ggtttaaagc ctttcagcag cgggttaaat accgtatgtg tcaggccgag
     8401 cgggcgataa atcgactctt caacatagcg gtcaagcggc tgaccggtaa ccgactcgac
     8461 gataaatcca agcagcatat aatcgacatc gctgtagata tgtttgctgc cgggctgata
     8521 ttccagcggc gtgcgcttga tcatctccag cgtctggcct ttatcctagg aatataacgc
     8581 gcccgcgacc gctttattcg ggtattgcgg atctgccggg aaaccgccgc tgtgatgcag
     8641 caggtcagaa atccgcaggg tgtttttgcc tttaatggtg tcatttggac tatcggcaaa
     8701 ccccggaata tatttcgcaa tccgatcgtc aggatgcagt ttgccttcgc tcatcagctt
     8761 ttgcagggcg aagttcgtgg cgtacatttt ggtgtttgag gccaggtcat acagcgtccc
     8821 ggtggtggct ttgacaggct gttccatcaa cacgctgcca tcgtactttt tcgccgctcc
     8881 ccaggctttg cgatacacaa tctgattatc tttaatgatc agcaggttta cgccgggata
     8941 accggcatca acttgctggc taatccagcg atccatctgg ttaagccgtt tgacgttaaa
     9001 ccctgctttc tctggcgagc tttctgtcag aacagggtat tttgcggcgc tgacgctaca
     9061 actaacagcc agcagagaaa gatagagcat tgtccgtttc atgatatcag tccagattga
     9121 cgttacggca gccaaagagc gtggtgaaaa taaacccgca aacccatgcc accagaatcc
     9181 cgccagcata aaccgccatg gccggaagga tgccttgtgc ggaagtcatc agcggcagcg
     9241 ctaccagacc agacggccca aaggcgctgt ttaagcccat cggcagaccc caccaggcta
     9301 tcaggccgat aaacaaaccg cccgccgcac cgcctaaaca ggcggtgaca aacggcttca
     9361 tgcggggcag ggtgacaccg taaatcagcg gttcaccaac gcccagcagg ccgggaataa
     9421 tcgccccgcg tacctgactg cgtagcgcac tgtgcggttg cgaccgccag tagagtgcca
     9481 gcgccgcgcc cacctggccc gcgcctgcca ttgaaaggat gggaaataag ctgttgaatc
     9541 cctggctgtc cattaacgcg aggtaaacag gaataaagcc ctgatgcacg ccaaatacca
     9601 cggcgatcag gaacagcccg gctaaaaccg cacaaccgaa cggattactg ttcaggtgca
     9661 taaacagcca tgacatacct tcgaataacc agccacccag cggcataatg atcaggtacg
     9721 caagcgtagc ggtgatcagc agggtgatta acgaggtcag cagcatgtcg agatcgtccg
     9781 gcataaagcg gcgcaccatg ccttcaatgc gggcgcaggc ccacgcggca atcagcacgc
     9841 cgataatatt gccgcgcgga tcgatgggca gaccaaagaa atcgtgaaaa ccggcgtagt
     9901 aaccggtggt tgcagcgggg ttataaccga gcaaaaagag tgcggcaata attgcgccat
     9961 tgacgcccgt gccgccaaat gcctgggcgg cgttatagcc caccagaatc accaggaagg
    10021 tgaacaaacc tttgctgaac accttcataa aattcagcgc gtcgggtagc gtgccctgag
    10081 catctgccgg aacgtgcatc accgtggcaa ttaacgtcgc tatccccagc agcagaccgg
    10141 cggcaataaa accggggatc agcggcgtga agatggtggc gaatttggcg agaaattgtt
    10201 gcacgccgga ggtttgtttg gcttttaact gacgtttgtt ctgggcggcg atttctgcgg
    10261 catcctgtac cggtgcctcg cccagcagct cgctcatcgc ttttgcggca cgatgcgctt
    10321 taccaggtcc aaaaacgacc tgcacctggt cgctggtcaa aatgacgctc ttcacgcctt
    10381 ccagcgtttt gatattggga tcaaccagtg aactgtcatg tacacccaga cgcaggcgcg
    10441 tcatacagtt accacaactg gcgatatttc ccggtccgcc gacacgggta agaatggtgt
    10501 tcagaagttc actgctgatc tctttggcca tgggttattc cttgtctaaa acctgacgaa
    10561 taaagccgcc gtgttgatcc aggcgttttt tagcttctgc ggcatcgaga tttttcagca
    10621 ccatcacaat ggccgtttta cagttgcgct cgcaggcaat taacgccgct tccgcttgtt
    10681 cggcattaca tccggtggcg tttttaacga tattgacctg gcgcacatgc agtttttcgt
    10741 tggtggcgac cacatcgacc ataaggttgc cgaacacttt gccggattta atcatcagcc
    10801 cggtggaaag catattgagc accagtttct gcgctgtacc tgctttcatc cgcgaagaac
    10861 cggtaacaac ttcggcacct acaatcggtg taatggcaaa ctcagcggtg gttgaaacgg
    10921 cgctccccgg attacaggaa attcccactg tgcggcagcc gagctggcgt gcgtattcca
    10981 gtccggcaat cacatacggc gtgcgaccgc tggcagcaat gccaaccacc acatcctgtg
    11041 ccgttaaatt aatatttttc agatcattaa caccgccttc ccggctatct tccgcgcctt
    11101 ccaccgcgtg ctgaatggca tattcgccgc cagcaatcaa accaaccacc agacccggtt
    11161 tcacgccgta ggtgggcgga cattcgctgg catccagaat ccccagacga ccggatgttc
    11221 ccgcaccgag gtaaatcaga cgcccgccgc cgcttacctg ggcgtggata acatcgatcg
    11281 ccgcggcgat atccggcagt acgcgctcaa cggcaagcgg tacggtttta tcttcatcgt
    11341 tgataatccg gcacatttcc agcgttgata cgcggtcaat ttcagccgag gcggcgttcg
    11401 agccttcagt aatcatcttt tcaagttgca tcggtggtga ccttactatt tacgatcaaa
    11461 taattgcaga tgtatggaat catatatcct gaattgacag acttaaagga atcattgagt
    11521 ctacactcgc agagtgtgaa gagcgtcata agaacttaag cggaatttat gctgccagcc
    11581 caggagagtg gaagatgttg tacctgacga aaataagcaa tgcggggagt gaattcaccg
    11641 aaaacgagca aaaaatcgct gactttctgc aggccaacgt gagtgagctg caatccgttt
    11701 cttcacggca gatggcgaaa cagctcggca ttagccagtc gagcatagtg aaatttgccc
    11761 aaaaactcgg cgcgcaagga tttactgaat tgcgtatggc gttaataggc gaatacagtg
    11821 ctagccggga gaaaacgaat accacggcgc tgcacctgca cagttcgatt accagtgatg
    11881 attcgctgga agtgattgcc cgcaaattga atcgcgaaaa agagctggcg ctggagcaaa
    11941 cctgtgcgtt gttcgactac gcgcggctgc aaaaaatcat tgaggtcatc agtaaagcac
    12001 cgtttatcca gataacgggc ctgggcggat cggcgctggt ggggcgcgat ctgtcgttca
    12061 aactgatgaa aattggctat cgcgtggcct gcgaagccga cacgcacgta caagccaccg
    12121 tttcccaggc gttgaaaaaa ggcgatgtac aaatcgctat ctcttacagc ggtagtaaaa
    12181 aagagattgt cttgtgcgta gaagcggcac gaaagcaggg ggcaacggtg attgccatta
    12241 cttctctggc tgactctccg ctgcggcggc tggcgcattt cacgctcgat accgtttccg
    12301 gtgaaaccga atggcgtagc tcgtcgatgt ctacccgcac ggcgcaaaat tctgttaccg
    12361 atttactgtt tgttggtctt gtgcaactca atgatgtggc gtcattgaaa atgattcagc
    12421 gcagcagtga gctaacacaa cgcctgaaat gatgcataaa gcagcgactg gattgagatt
    12481 ttcctgaatt agtgagctga tccgcagcaa tattttgttt atcctgtatt ttcagaggga
    12541 atggagtgta acgctctgta ttaacaagga gagcattaaa atgggtaaac tcacgggcaa
    12601 gacagcactg attacgggcg cattgcaggg aattggcgaa ggaattgcca gaacttttgc
    12661 acgtcatggc gcgaacctaa tcttgctgga tatctcccct gagatcgaaa agctggcgga
    12721 cgaactgtgt ggtcgtggtc atcgctgtac ggcggttgtc gccgatgtgc gtgacccggc
    12781 gtcggtagcc gcagctatca aacgcgcgaa ggaaaaagaa gggcgcattg atatcctggt
    12841 gaataacgca ggcgtttgtc gtctgggcag tttcctcgat atgagcgatg acgatcgcga
    12901 tttccatatt gatatcaata ttaaaggcgt atggaacgtc acgaaggcgg tgctgccgga
    12961 gatgattgcg cgcaaagatg gtcgcattgt gatgatgtct tcagtcactg gtgatatggt
    13021 ggccgatcct ggcgaaacgg cgtacgcctt aacgaaagcg gcgattgttg gcctgacaaa
    13081 atcgctggcg gtggagtacg cgcagtctgg tattcgcgtt aacgccattt gcccgggata
    13141 cgtgcgcaca ccaatggcgg aaagcattgc ccgccagtcg aacccggaag atccagagtc
    13201 ggtgctgact gaaatggcga aagcaatccc gatgcgtcgc ctcgccgatc cgctggaagt
    13261 cggcgaactg gcggccttcc tcgcatcgga tgaatccagc tatttaaccg gtacacagaa
    13321 tgtgattgat ggcggcagca cactgccgga gacggttagc gtcggtatct gattcacctc
    13381 tgtttcctcc ctgcatgtgt ggggaggatt tcgtcttgaa ctaagttcac caggctattt
    13441 tatttgtcat tttggccccg ggcagtgctc gaaatactca cgtactatgt gtacgctccg
    13501 gtttctccgc gctgtccgtg tccaaactgc ccgcaacaat tacgcctgtt gaaccaagtt
    13561 cttattccct ttttaacttc caaatcacca aacggtatat aaaaccgtta ctcctttcac
    13621 gtccgttata aatatgatgg ctattagaaa gtcattaaat ttataagggt gcgcaatggc
    13681 cgttaactta ctgaaaaaga actcactcgc gctggtcgct tctctgctgc tggcgggcca
    13741 tgtacaggca acggaactgc tgaacagttc ttatgacgtc tcccgcgagc tgtttgccgc
    13801 cctgaatccg ccgtttgaac agcaatgggc aaaagataac ggcggcgaca aactgacgat
    13861 aaaacaatct catgccgggt catcaaaaca ggcgctggca attttgcagg gcttaaaaac
    13921 cgacgttgtc acttataacc aggtgaccga cgtacaaatt ctgcacgaca aaggcaagct
    13981 gatcccggct gactggcagt cgcgcctgcc gaataatagc tcgccgttct actccaccat
    14041 gggcttcctg gtgcgtaagg gcaacccgaa gaatatccac gactggaacg acctggtgcg
    14101 ctccgacgtg aagctgattt tcccgaaccc gaaaacgtcg ggtaacgcgc gttataccta
    14161 tctggcggca tggggcgcag cggataaagc tgacggtggc gacaaagcca aaaccgaaca
    14221 gtttatgacc cagttcctga aaaacgttga agtgttcgat actggcggtc gtggcgcgac
    14281 caccactttt gccgagcgcg gcctgggcga tgtactgatc agcttcgagt cggaagtgaa
    14341 caacatccgt aaacagtatg aagcgcaagg ctttgaagtg gtgattccga aaaccaacat
    14401 tctggcggaa ttcccggtgg cgtgggtcga taaaaacgtg caggccaacg gtacggaaaa
    14461 agcagcaaaa gcctacctga actggctcta cagcccgcag gcgcagacca tcatcaccga
    14521 ctattactac cgcgtaaata acccggaagt catggacaaa ctgaaagata aattcccgca
    14581 gaccgagctg ttccgcgtgg aagacaaatt tggctcctgg ccggaagtga tgaaaaccca
    14641 cttcaccagc ggcggcgagt tagacaagct gttagcggcg gggcgtaact gatgtttgca
    14701 gtttcatcaa gacgcgtgct gccgggcttt accttaagcc tcggcaccag tctgctgttt
    14761 gtgtgcctga ttttgctgct gccgctctcc gcgctggtga tgcagctggc tcagatgagc
    14821 tgggcgcagt actgggaggt gatcaccaac ccacaagtgg tcgcggccta caaagtgacg
    14881 ctgctgtcgg cgtttgtggc atcgattttt aacggcgttt tcggtctgct gatggcgtgg
    14941 attttaacac gttatcgctt tccgggccgc acgctgcttg atgcgctgat ggatttaccc
    15001 tttgcgctgc caacggcagt cgccggttta acgctggcct cgctcttttc cgtaaacggt
    15061 ttttacggtg aatggctggc gaagtttgat atcaaagtca cctatacctg gcttggtatt
    15121 gcggtggcga tggcctttac cagcattccg tttgtggtgc gtaccgtgca gccggtgctg
    15181 gaagagttag gcccagaata tgaagaagcg gcggaaacgc ttggtgcaac gcgctggcag
    15241 agtttctgca aagtggtgct gccggaactt tctccagcgc tggtggcggg cgtggcgttg
    15301 tcgtttaccc gtagtcttgg tgagtttggc gcggtgatat ttatcgccgg gaacatcgct
    15361 tggaagacgg aagtgacgtc gctgatgatt tttgtgcgct tgcaggagtt tgattacccg
    15421 gcggcgagcg cgattgcttc ggtgatcctc gcggcatccc tgctgctgct gttctcaatt
    15481 aacactctgc aaagtcgctt tggtcggcgt gtggtaggtc attaatggcg gaagttaccc
    15541 aattgaagcg ttatgacgcg cgcccgatta actggggcaa atggtttctg attggcatcg
    15601 ggatgctggt atcggcgttc atcctgctgg tgccgatgat ttatatcttc gtacaggcgt
    15661 tcagcaaagg gctgatgccg gttttacaga atctggccga tccggacatg ctgcacgcca
    15721 tttggctgac ggtgatgatt gcgctgattg ccgtaccggt gaacctggtg ttcggcattc
    15781 tgctggcgtg gctggtaacg cgctttaact tccccggacg ccagttactg ctgacgctac
    15841 tggacattcc gtttgccgta tcgccggtag ttgccggtct ggtgtatctg ctgttctacg
    15901 gctctaacgg tccgctgggc ggctggctgg atgaacataa tttgcagatt atgttctcct
    15961 ggccgggaat ggtgctggtc accatctttg tgacctgtcc ttttgtggtg cgcgagctgg
    16021 tgccggtgat gttaagccag ggcagccagg aagacgaagc ggcgattttg cttggcgcgt
    16081 ctggctggca gatgttccgt cgcgtcacat tgccgaacat tcgctgggcg ctgctgtatg
    16141 gtgtggtgct aaccaacgcc cgcgcgattg gcgagtttgg tgcggtgtcg gtggtttccg
    16201 gctcgattcg cggcgaaaca ctttcgctgc cgttacagat tgaattgctg gagcaggact
    16261 acaacaccgt cggctccttt accgccgccg cgctgttgac gctgatggcg attatcaccc
    16321 tgtttttaaa gagtatgttg cagtggcgtc tggagaatca ggaaaaacgc gcgcagcagg
    16381 aggagcatca tgagcattga gattgccaat attaagaagt cgtttggtcg cacccaggtg
    16441 ctgaacgata tctcactgga tattccttct ggtcagatgg tcgcgctgct ggggccgtcc
    16501 ggttccggaa aaaccacgct gctgcgcatt attgccgggc tggagcatca aaccagcggg
    16561 catattcgct tccacggtac cgacgtgagc cgcctgcacg cacgcgatcg taaagtcggt
    16621 ttcgtgttcc agcattacgc gctgttccgt catatgacgg tgttcgacaa tatcgctttt
    16681 ggcctgacgg tgctgccacg tcgcgagcgc ccgaatgccg cagccatcaa agcgaaagtg
    16741 acaaaattgc tggagatggt ccagcttgcg catctggcgg atcgttatcc ggcacagctt
    16801 tccggcggcc agaaacagcg cgtggcgctg gcgcgcgcgc tggctgttga accgcaaatt
    16861 ctcctgcttg atgaaccgtt tggcgcgctg gatgcgcagg tgcgtaaaga gctgcgtcgc
    16921 tggctgcgtc aactgcatga agagctgaaa ttcaccagcg tgttcgtgac ccacgaccag
    16981 gaagaagcga ccgaagtagc tgatcgtgta gtggtgatga gccagggcaa tatcgagcag
    17041 gctgatgcgc cggaccaggt atggcgcgaa ccagcgaccc gatttgtgct cgaatttatg
    17101 ggcgaagtga accgcctgca gggaaccatt cgcggcgggc agttccatgt tggcgcacat
    17161 cgctggccgc tggggtatac gcctgcgtat caggggcagg tggatctctt cctgcgcccg
    17221 tgggaagtgg atatcagccg ccgtaccagt ctcgattcgc cgctgccggt acaggtactg
    17281 gaagccagcc cgaaaggtca ctacacccaa ttagtggtgc agccgctggg gtggtacaac
    17341 gaaccgctga cggtcgtgat gcatggcgac gatgccccgc agcgtggcga gcgtttattc
    17401 gttggtctgc aacatgcgcg gctgtataac ggcgacgagc gtatcgaaac ccgagatgag
    17461 gaacttgctc tcgcacaaag cgcctgatag gttgagtgaa tgttaaacgc ccggaggcgc
    17521 ttcccgcgat ccgggctttt taatggcaag gtttgtaacc tgtagacctg ataagacgcg
    17581 caagcgtcgc atcaggcaac accacgtatg gatagagatc gtgagtacat tagaacaaac
    17641 aataggcaat acgcctctga taaagttgca gcgaatgggg ccgaataacg gcagtgaagt
    17701 ctggttgaaa ctggaaggca ataacccggc aggttcggtg aaagatcgtg cggcactttc
    17761 gatgatcgtc gaggcggaaa agcgcgggga aattaaaccg ggtgatgtgt taatcgaagc
    17821 caccagtggt aacaccggca ttgcgctggc aatgattgcc gcgcttaaag gctatcgcat
    17881 gaaattgctg atgcccgaca acatgagcca ggaacgccgt gcggcgatgc gtgcttatgg
    17941 tgcggaactg attcttgtca ccaaagagca gggcatggaa ggtgcgcgcg atctggcgct
    18001 ggagatggcg aatcgtggcg aaggaaagct gctcgatcag ttcaataatc ccgataaccc
    18061 ttacgcccat tacaccacta ccgggccgga aatctggcag caaaccggcg ggcgcattac
    18121 ccattttgtc tccagcatgg gaacaaccgg cactatcacc ggcgtttcac gctttatgcg
    18181 cgaacaatcc aaaccggtga ccattgtcgg cctgcagccg gaagaaggca gcagtattcc
    18241 cggcattcgc cgctggcctg cggaatatct gccggggatt ttcaacgctt ctctggtgga
    18301 tgaggtgctg gatattcatc agcgcgatgc ggaaaacacc atgcgcgaac tggcggtgcg
    18361 ggaaggaata ttctgtggcg tcagctcagg cggcgcggtt gctggagcac tgcgggtggc
    18421 aaaagctaac cctggtgcgg tggtggtagc gatcatctgc gatcgtggcg atcgctacct
    18481 ctccaccgga gtgtttgggg aagagcattt tagccagggg gcggggattt aagattattg
    18541 cgcaggcacc gttttatccg tctgcgcaat tagcgcatgt aaaaaaggct gtgcgttctc
    18601 atcgctctta ccggggatct tcacaatata cggcttcccg gtaatcgatg acgacgaagc
    18661 aaccttatca ataaactgct cggcagtgtc aatgcggtta cgggtattgc cgagcttcag
    18721 acgcagatga gaaaccgctt catcgcaggt atgttcatca ccgttgcgca caaatatcaa
    18781 atcctttttc tgtgataatc cctccagcat ggcgttgatg cgggcttctt catgggcagt
    18841 taacttcgcg taagcgggga gtgtcattag tagtgtaatg accagacaaa tgattttctt
    18901 cattgaatta ccttatcctt gttttgtaat agccgatttg actgccagaa tcaggtaaag
    18961 tttcggttgg aatacgatga ggatcatcgg caaggagaaa aaattaaatg agtagtttgt
    19021 tgttgtttaa cgataagagc agggcgctgc aggcggatat cgtcgccgtg cagtcgcagg
    19081 tggtttacgg cagcgtgggc aacagcattg ccgtgcctgc tatcaaacag aacggtctga
    19141 atgtttttgc cgtgccgacg gtattgctga gcaatacgcc gcattatgac actttctacg
    19201 gtggtgcgat tccggatgaa tggtttagcg gttatttgcg tgcgcttcag gagcgtgatg
    19261 cgctgcgcca gctgcgcgct gtaaccacgg gctatatggg aacggcatcg caaatcaaaa
    19321 tccttgccga gtggctgact gcgctacgca aagaccatcc tgacctattg atcatggtcg
    19381 atccggtgat tggcgatatt gatagcggaa tttatgtcaa acctgacctt cccgaggcgt
    19441 atcgacaata tttactgccg ctggcgcagg gaattacccc caatatcttt gagctggaaa
    19501 tcctgaccgg taaaaattgc cgcgatctcg acagtgccat tgctgccgca aaaagtctgc
    19561 tttcagacac attaaaatgg gtggtgatta ccagcgcctc cggtaatgaa gaaaatcagg
    19621 agatgcaggt ggtggtggtc agtgccgaca gcgtgaatgt catttcccat tcacgggtaa
    19681 aaaccgacct gaaagggact ggcgacctgt tttgtgctca gcttatcagt ggtttgctga
    19741 aagggaaggc gttaaacgat gcggtgcacc gtgcggggtt gcgcgtactg gaagtgatgc
    19801 gctacaccca gcagcatgag agcgatgaat tgattttgcc gccgctggcg gaagcataaa
    19861 aaatggcgcc gatgg
//
LOCUS       DAJVUJ010000070        19773 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.070, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000070 DAJVUJ010000000
VERSION     DAJVUJ010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19773
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.070"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            49..993
                     /gene="wecH"
                     /locus_tag="OQQ48_002238"
     CDS             49..993
                     /gene="wecH"
                     /locus_tag="OQQ48_002238"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000261200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HCS1952996.1"
                     /translation="MVVMIHTTTWYVTNAHSVSPVTWDIANVLNSASRVSVPLFFMIS
                     GYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINMELALKNLLQKPVFYHLW
                     FFFAIAVIYLVSPLIQVKNVGGKMLLVLMAVIGIIANPNTVPQKIDGFEWLPINLYIN
                     GDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFIISRGTLYELQWRGNFADT
                     WYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLGIYGFHALIIHALRTRGI
                     ELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(1035..1472)
                     /locus_tag="OQQ48_002239"
     CDS             complement(1035..1472)
                     /locus_tag="OQQ48_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HCS1952997.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(1518..1859)
                     /locus_tag="OQQ48_002240"
     CDS             complement(1518..1859)
                     /locus_tag="OQQ48_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1952998.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(2024..2215)
                     /locus_tag="OQQ48_002241"
     CDS             complement(2024..2215)
                     /locus_tag="OQQ48_002241"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1952999.1"
                     /translation="MGPALGAARLAQIAANPEKSLIELLPQLPLEQSHLPDAQRYAAY
                     QPRRETFRRLYQQLLPLMA"
     gene            complement(2148..3467)
                     /gene="xylB"
                     /locus_tag="OQQ48_002242"
     CDS             complement(2148..3467)
                     /gene="xylB"
                     /locus_tag="OQQ48_002242"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HCS1953000.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHSFCH
                     ALPQRWHLMSVMLSAASCLDWVAKLTGLCNVPALIAAAQQADESAEPVWFLPYLSGER
                     TPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTLIGG
                     GARSEYWRQMLADISGQQLDYRTGGCGASTGRSKAGADCGESREIAH"
     gene            complement(3584..4861)
                     /gene="xylA"
                     /locus_tag="OQQ48_002243"
     CDS             complement(3584..4861)
                     /gene="xylA"
                     /locus_tag="OQQ48_002243"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HCS1953001.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVSFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAAKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGR"
     gene            5227..6198
                     /gene="xylF"
                     /locus_tag="OQQ48_002244"
     CDS             5227..6198
                     /gene="xylF"
                     /locus_tag="OQQ48_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HCS1953002.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNGETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALTANNNKIDAVVASNDATAGG
                     AIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAEIAVELGN
                     GQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            6276..7817
                     /gene="xylG"
                     /locus_tag="OQQ48_002245"
     CDS             6276..7817
                     /gene="xylG"
                     /locus_tag="OQQ48_002245"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HCS1953003.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            7795..8976
                     /gene="xylH"
                     /locus_tag="OQQ48_002246"
     CDS             7795..8976
                     /gene="xylH"
                     /locus_tag="OQQ48_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HCS1953004.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            9054..10232
                     /gene="xylR"
                     /locus_tag="OQQ48_002247"
     CDS             9054..10232
                     /gene="xylR"
                     /locus_tag="OQQ48_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XylR"
                     /protein_id="HCS1953005.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNREVML
                     "
     gene            complement(10340..11164)
                     /locus_tag="OQQ48_002248"
     CDS             complement(10340..11164)
                     /locus_tag="OQQ48_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HCS1953006.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSRSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            11484..13514
                     /gene="malS"
                     /locus_tag="OQQ48_002249"
     CDS             11484..13514
                     /gene="malS"
                     /locus_tag="OQQ48_002249"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCS1953007.1"
                     /translation="MKLASCFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLINKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASSALREWKKTNPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            13692..14945
                     /gene="avtA"
                     /locus_tag="OQQ48_002250"
     CDS             13692..14945
                     /gene="avtA"
                     /locus_tag="OQQ48_002250"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HCS1953008.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNVFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(15096..15569)
                     /locus_tag="OQQ48_002251"
     CDS             complement(15096..15569)
                     /locus_tag="OQQ48_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCS1953009.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(15671..16519)
                     /gene="yiaJ"
                     /locus_tag="OQQ48_002252"
     CDS             complement(15671..16519)
                     /gene="yiaJ"
                     /locus_tag="OQQ48_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HCS1953010.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGAIT"
     gene            16720..17718
                     /gene="yiaK"
                     /locus_tag="OQQ48_002253"
     CDS             16720..17718
                     /gene="yiaK"
                     /locus_tag="OQQ48_002253"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HCS1953011.1"
                     /translation="MKVTFEQLKAAFNRALISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            17730..18198
                     /locus_tag="OQQ48_002254"
                     /pseudo
     CDS             17730..18198
                     /locus_tag="OQQ48_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
     gene            18316..18789
                     /gene="yiaM"
                     /locus_tag="OQQ48_002255"
     CDS             18316..18789
                     /gene="yiaM"
                     /locus_tag="OQQ48_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter small
                     permease YiaM"
                     /protein_id="HCS1953012.1"
                     /translation="MKKILEVILAINLAVLSCIVFINIILRYGFQTSILSVDELSRYL
                     FVWLTFIGAIVAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWGATLK
                     TIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQGA"
     gene            18792..>19773
                     /locus_tag="OQQ48_002256"
     CDS             18792..>19773
                     /locus_tag="OQQ48_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HCS1953013.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLSKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPDMMMGATLMLTWWWQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSTWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVG"
BASE COUNT         4989 a         4952 c         4805 g         5027 t
ORIGIN      
        1 cagcccaaaa tttactggat tgataacctg cgagggatag cgtgtttaat ggtggtgatg
       61 attcacacca ctacctggta tgtgaccaat gctcatagtg ttagccccgt cacatgggat
      121 atcgccaatg ttctgaattc tgcctctcgt gtcagcgtgc cgctattttt catgatttcc
      181 ggctatctct tttttggcga acgcagcgcc cagccgcgcc atttcttgcg tatcggctta
      241 tgtctgattt tttatagcgc aatcgcactg ctctacattg cgctgtttac ctccatcaat
      301 atggagttag cgctgaaaaa cctgctgcaa aagccagtgt tttaccactt gtggtttttc
      361 ttcgcgattg cggtgattta tctggtttca ccgctgattc aggtgaagaa cgtcggcgga
      421 aaaatgttgc tggtgctaat ggcggtgatt ggcattatcg ctaacccaaa cacagtgccg
      481 cagaaaattg acggttttga atggctgcca attaacttat atatcaatgg cgatactttt
      541 tactacattc tgtatggcat gttgggccgc gctataggga tgatggacac acagcataaa
      601 gcactgtcgt gggtgagcgc cgcgctgttt gcgacggggg tttttattat ctctcgcggg
      661 acattatatg aattgcagtg gcgcggaaat tttgccgata cctggtatct ttactgtggg
      721 ccgatggttt ttatctgcgc aatcgcgcta ttgactctgg ttaaaaacac gctggatacg
      781 cgtaccattc gcggacttgg cttaatctcc cgccattcat tgggtatata cggattccac
      841 gccttgatta tccatgcgct gcgcacccgg ggaattgagc ttaaaaattg gccaatactg
      901 gatattattt ggatcttttg cgcgacgttg gcagcgagtt tgttactttc tatgctggta
      961 caacgaatcg acagaaacag attagtgagt taagtaaaag cccgttcaca ttggactgac
     1021 cgggcttacg tgaattattc gctgtattct tcctgggtaa cctgtgtttc ttttggcggg
     1081 tttattccgg catcacgaat attcttctgc accgctactg caccaaacaa gcttaagaag
     1141 aaagccagtc cataaaaacc tttttcactg agtaataatg tcgcattcca cagacctacc
     1201 atcagtaatg caacagagat aataaagaca gtcaggcagg tcatataata aatggaagtg
     1261 gtcggtatgc cttcatactt gtcccgaacg gtcttttgat aagacgcagc agaaaacagt
     1321 cctaacacca gtacggcaaa ataataccct ttttcattta actgcatctc tgcattccat
     1381 agccctaaca gataggtaac gataccacca acgagagcta tccatgacac aatactaaag
     1441 gccggtgaat aggttgatat tttgttgtcc atcacagtat tcctttcatt cctgaatgtg
     1501 taagagcttt atgttgctca taccgagcga aacaaacgca tttgaccaaa caagcagaca
     1561 aaaaaggcga tggggtagat caccatttca taagcattga taggtgcatt taaaacaccg
     1621 ataaacaaga gaccgatagt gattaacgca actaactggc agatatgggt aaaaaaatta
     1681 tccagttgcc cgagtttttc tgcgcgaaga tatgcatagt taccaaaagt tgctgtcatt
     1741 aacacgccaa gaaaatagcc ttttccactt agcaatgggc atgagatcca tagcccaacc
     1801 agataaacca gcgccccgac aacaaataat agttttgcca cagtttttga ggttttcata
     1861 acgatctcca tatctaccag cgatacatta cgagtaacca acgaaagaca aaactgaaaa
     1921 atgccattaa caaatgattt tcagaataaa ttcatactaa atattaatta gtgagatata
     1981 tagatgtgaa ttatccccca cccggtcagg caggggataa cgtttacgcc attaatggca
     2041 gaagttgctg atagaggcga cggaacgttt cacgtcgtgg ctgataagcg gcataacgct
     2101 gcgcatctgg tagatgcgac tgttctaacg gtagctgcgg caacaactca atgagcgatt
     2161 tctctggatt cgccgcaatc tgcgccagcc ttgctgcgcc cagtgctggc cccacatccc
     2221 cccgtacggt aatcgagctg ctgaccgctg atatccgcca gcatctgacg ccagtactca
     2281 ctacgcgccc cgccaccaat caacgtaaca ctttgcggtt taatgccgca ggcatgcacg
     2341 acatccatgc catctgccag cgcataaccc acgccttcca gcactgctcg cgccagttca
     2401 ttggggccat gttgatgagt caaaccaaag aaaaccccct tcgcctgggg attattgtgt
     2461 ggtgtacgct cgccggaaag ataaggcaga aaccaaactg gctcggcact ttcatcagcc
     2521 tgttgagctg cagcgattaa agctgggaca ttgcacaggc cggttaattt cgcgacccaa
     2581 tccagacacg atgctgcact cagcattaca gacattaaat gccaacgctg cggtagcgca
     2641 tggcaaaagc tgtgtacagc gctttctggc ttgcttaaga acccttcgct gacagcaaaa
     2701 tagacccccg acgtccccag cgataacatt gcctgattag catcaaccat tcccacacca
     2761 actgcattgt cgccaccgcc tgcgacaact ggcaccgtcg ccatacccca cgctttcgca
     2821 acttcaggta acaaagcacc agtaatttcg ctgccttcgt ataatgcggg catctggtca
     2881 cgagataagt cgcaagcctg cagcatgacg tcactccagt cacgctttgc gacatccagc
     2941 cacatggtgc cagctgcgtc agacatatcg ctggcaaact cccccgtcat acgcagacgc
     3001 aagtaatctt tcggtaataa tactttgtcg atttgacgga atatctccgg ctcatgccgc
     3061 tgaacccata gcaatttagg cgcagtaaat ccgggcatca tcaggttgcc ggtaatcact
     3121 cgtgattgcg gaactctcgc ttccagcaaa gtgcactctt gcgcacagcg cccgtcgttc
     3181 cacaaaatgg cagggcgcaa tacccgttgt tgagcatcca gtaaggttgc tccatgcatc
     3241 tggccggcaa tacccaatgc tttaacgtcc tgcagagaat gctgatcgcc cagagctttc
     3301 attgcgcgat cagttgcctg ccaccactgt tccgggtctt gttccgacca gagtggatgc
     3361 gggcgcgaaa cggtcagctt ttccgtttgc gaagcaacca cctcaccctg ctcgttgagc
     3421 aaaataactt ttacgcccga ggtgccaaga tctatcccga tatacatatc gatcgttcct
     3481 taaaaaaatg cccggtatcg ctaccgataa ccgggccaac ggactgcaca gttagccgtt
     3541 atttgtcgaa cagataatga tttaccagat tttccagttg ctcctagcga ccactctgat
     3601 gcaccggaga caaattatgt tcctgagcat atttggctaa atctgccagt gacatttggc
     3661 ctttcaggat ttgctggccc aattcgctat tccagccgga ataacgctgc gcgatgcgtt
     3721 tatccagctc gccatcttca atcatgcgcg ctgcaatttt cagcgccagt gccatcgtat
     3781 ccatcgcgcc gatatgaccg taaaacagat catatttatc agtactttga cgacgtactt
     3841 tggcatcgaa gttcagacca ccggtggtga aaccgcctgc tttgagaatt tcatacatca
     3901 ccagcgcatt ctcttccaca ctgttcggga actggtcggt gtcccagccc agttgcgcat
     3961 cgccacggtt ggcgtcgaca gaaccgaaca ggccaagcgc aatggcggtg gctatttcat
     4021 gatggaaaga gtgacctgcc agcgtcgcgt ggttagcttc aatgttcagt ttaatctctt
     4081 tttccagacc aaactgtttc aggaagccat agaccgtcgc ggcatcgtaa tcatattgat
     4141 gtttagtcgg ttcttgcggt ttcggttcga taagcaacgt gccctggaag ccgattttat
     4201 gtttatgctc aaccaccatc tgcataaagc ggcccagttg ttcacgctcc tgacgcaagt
     4261 cggtatttaa cagcgtttcg taaccttcac gaccacccca caggacatag ttttcaccgc
     4321 ccaatttatg ggttgcttcc atcgctgtaa caacttgcgt tgccgcccag ctgaagactt
     4381 caggatctgg gttcgtcgcc gcacccgcgc catagcgagg gtttgtaaag cagttagcgg
     4441 ttccccacag cagcttcacg ccgctctctt cttgcttggc tgccaggaca tcaaccattt
     4501 gcgcaaaatt attgatgtac tcttttaacg acgcgccctc aggggaaaca tccacatcgt
     4561 ggaagcaata aaatgacaca tgtaacttgt ggaaaaactc aaatgcgaca tctgctttac
     4621 gcttcgccaa cgccagtgcc tcaccaggct gctgccacgg acgattaaac gcccccacac
     4681 caaacatgtc cgccccgttc cagcagaagg tgtgccagta acaggcggca aaacgcaagt
     4741 gctcttccat acgcttaccc aacaccagtt cgtcgggatt gtagtgacgg aatgctaacg
     4801 ggtttgagga ttttgagcct tcataacgaa cgcgatcgag ctggtcaaaa taggcttgca
     4861 tattgaactc cataatcagg taatgccgcg ggtgatggat gatgtcgtaa tattgggcac
     4921 tccctttcag ttgctcaatt atgttatttc acactgctat tgagataatt cacaagtgtg
     4981 cgctcgctcg caaaataaaa tgggatgatg aaactgggta attcctcgaa gataaaaatg
     5041 caataagtac aattgcgcaa caaaagtaag atctcggtca taaatcaaga aataaaccaa
     5101 aaatcgtaat cgaaagataa aaatctgtaa ttgttttccc ctgtttagtt gctaaaaatt
     5161 ggttacgttt atcgcggtga ttgttactta ttaaagctgt cctctaacta cagaaggccc
     5221 tacaccatga aaataaagaa cattctactc accctttgca cctcacttct gcttaccaac
     5281 gttgcggcac acgccaaaga agtcaaaata ggtatggcga ttgatgatct acgtcttgaa
     5341 cgctggcaaa aagatcgaga tatttttgtg aaaaaggctg aatctctcgg tgcgaaagta
     5401 tttgtacagt ctgcaaatgg caatggagaa acacaaatgt cgcagattga aaacatgatt
     5461 aaccggggtg tcgatgttct tgtcattatt ccgtataacg gccaggtatt aagtaacgtt
     5521 gtaaaagaag ccaaacaaga aggtattaaa gtattagctt acgaccgtat gattaacgat
     5581 gcggatatcg atttttatat ttctttcgat aacgaaaaag tcggtgaact gcaggcaaaa
     5641 gccctggtcg atattgttcc gcaaggtaat tacttcctga tgggcggctc gccggtagat
     5701 aacaacgcca agctgttccg cgccggacaa atgaaagtgt taaaacctta cgttgattcc
     5761 ggaaaaatta aagtcgttgg tgaccaatgg gttgatggct ggttaccgga aaacgcgcta
     5821 accgccaata ataacaaaat tgatgctgta gttgcctcaa acgatgccac cgcaggtggg
     5881 gcaattcagg cattaagcgc gcaaggttta tcagggaaag tagcaatttc cggccaggat
     5941 gcggatctcg caggtattaa acgtattgct gccggtacgc aaactatgac ggtgtataaa
     6001 cctattacat tgctggcaaa tactgccgca gaaattgccg ttgaattggg caatggtcag
     6061 gagccaaaag cagatacctc actgaataat ggcctgaaag atgtcccctc ccgcctcctg
     6121 acaccgatcg atgtgaataa aaacaacatc aaagatacgg taattaaaga cggattccac
     6181 aaagagagcg agctgtaagc gttacgcccc ggcgcggagc gggggcgtga tttctctcca
     6241 tgccgcgtga atgaattggc ttaggtggag tcgttatgcc ttatctactt gaaatgaaga
     6301 acattaccaa aaccttcggc agtgtgaagg cgattgataa cgtcagcttg cggttgaatg
     6361 ctggcgaaat cgtctcactc tgtggggaaa atgggtctgg taaatcaacg ctgatgaaag
     6421 tgctgtgtgg tatttatccc catggctcct acgaaggcga aattattttt gcgggagaag
     6481 agattcaggc gagtcacatc cgcgataccg aacgcaaagg tatcgccatc attcaccagg
     6541 aattggccct ggtgaaagaa ttgaccgtgc tggaaaatat cttcctgggt aacgaaataa
     6601 cccacaatgg cattatggat tatgacctga tgacgctacg ctgtcagaag ctgctcgcac
     6661 aggtcagttt atccatttca cctgataccc gcgttggcga tttagggctt gggcaacaac
     6721 aactggttga aattgccaag gcacttaata aacaggtgcg cttgttaatt ctcgatgaac
     6781 cgacagcctc attaactgag caggaaacgt cggttttact ggatattatt cgcgatctac
     6841 aacagcacgg tatcgcctgt atttatattt cgcacaaact caacgaagtc aaagcgattt
     6901 ccgatacgat ttgcgttatt cgcgacggtc agcacattgg tacgcgtgat gctgccggaa
     6961 tgagtgaaga cgatattatc accatgatgg tcgggcgaga gttaaccgcg ctttacccta
     7021 atgaaccaca taccaccgga gatgaaatat tacgtattga acatctgacg gcatggcatc
     7081 cggttaatcg tcatattaaa cgagttaatg atgtctcgtt ttccctgaaa cgtggcgaaa
     7141 tactgggtat tgccggactc gttggtgccg gacgtaccga gaccattcag tgcctgtttg
     7201 gcgtgtggcc cggacaatgg gaaggaaaaa tttatattga tggcaaacag gtagatattc
     7261 gtaactgtca gcaagccatc gcccagggga ttgcgatggt acccgaagac agaaagcgcg
     7321 acggcatcgt tccggtaatg gcggttggta aaaatattac cctcgccgca ctcaataaat
     7381 ttaccggtgg cattagccag cttgatgacg cggcagagca aaaatgtatt ctggaatcaa
     7441 tccagcaact caaagttaaa acgtcgtccc ccgatcttgc tattggacgt ttgagcggcg
     7501 gcaatcagca aaaagcgatc ctcgcccgct gtctgttact caacccgcgc attctcattc
     7561 ttgatgaacc taccaggggt attgatattg gcgcgaaata tgagatctac aaattaatta
     7621 accaactcgt ccagcagggt attgccgtta ttgtcatctc ttccgaatta cctgaagtgc
     7681 tcggccttag cgatcgtgta ctggtgatgc atgaagggaa actaaaagcc aacctgataa
     7741 atcataacct gactcaggag caggtgatgg aagccgcatt gaggagcgaa catcatgtcg
     7801 aaaagcaatc cgtctgaagt gaaattggcc gtaccgacat ccggtggctt ttccgggctg
     7861 aaatcactga atttgcaggt cttcgtgatg attgcagcta tcatcgcaat catgctgttc
     7921 tttacctgga ccaccgatgg tgcctactta agcgcccgta acgtctccaa cctgttacgc
     7981 cagaccgcga ttaccggcat cctcgcggta ggaatggtgt tcgtcataat ttctgctgaa
     8041 atcgaccttt ccgtcggctc aatgatgggg ctattaggtg gcgtcgcggc gatttgtgac
     8101 gtctggttag gctggccttt gccacttacc atcattgtga cgttggttct gggactgctt
     8161 ctcggtgcgt ggaacggatg gtgggtcgcg taccgtaaag tcccttcatt tattgtcacc
     8221 ctcgcgggca tgttggcatt tcgcggcata ctcattggca tcaccaacgg cacgactgta
     8281 tcccccacca gcgccgcgat gtcacaaatt gggcaaagct atctccccgc cagtaccggc
     8341 ttcatcattg gcgcgcttgg cttaatggct tttgttggtt ggcaatggcg cggaagaatg
     8401 cgccgtcagg ctttgggttt acagtctccg gcctctaccg cagtagtcgg tcgccaggct
     8461 ttaaccgcta tcatcgtatt aggcgcaatc tggctgttga atgattaccg tggcgttccc
     8521 actcctgttc tgctgctgac gttgctgtta ctcggcggaa tgtttatggc aacgcggacg
     8581 gcatttggac gacgcattta tgccatcggc ggcaatctgg aagcagcacg tctctccggg
     8641 attaacgttg aacgcaccaa acttgccgtg ttcgcgatta acggattaat ggtagccatc
     8701 gccggattaa tccttagttc tcgacttggc gctggttcac cttctgcggg aaatatcgcc
     8761 gaactggacg ctattgcagc atgcgtgatt ggcggcacca gcctggctgg cggtgtggga
     8821 agcgttgccg gagcagtaat gggggcattt atcatggctt cactggataa cggcatgagt
     8881 atgatggatg taccgacctt ctggcagtat atcgttaaag gtgcgattct gttgctggca
     8941 gtatggatgg actccgcaac caaacgccgt tcttgatttt gataaaaatt ttctcaaagc
     9001 cggttacgta ttaccggttt tgagtttttg catgattcag caggaaaaga accatgttta
     9061 ctaaacgtca ccgcatcaca ttactgttca atgccaataa agcctatgac cggcaggtag
     9121 tagaaggcgt aggggaatat ttacaggcgt cacaatcaga atgggatatt ttcattgaag
     9181 aagatttccg cgcccgcatt gataaaatca aggactggtt aggagatggc gtcattgccg
     9241 acttcgacga caaacaaatc gagcaagcgc tggctgatgt cgacgtcccc attgttgggg
     9301 ttggcggctc gtatcacctt gcagaaagtt acccacccgt tcattacatt gccaccgata
     9361 actatgcgct ggttgaaagc gcatttttgc atttaaaaga gaaaggcgtt aaccgctttg
     9421 ctttttatgg tcttccggaa tcaagcggca aacgttgggc cactgaacgc gaatatgcat
     9481 ttcgtcagct tgtcgccgaa gaaaagtatc gcggagtggt ttatcagggg ttagaaaccg
     9541 cgccagagaa ctggcaacac gcgcaaaatc ggctggcaga ctggctacaa acgctgccac
     9601 cgcaaaccgg gattattgcc gttactgacg cccgggcgcg gcatattctg caagtatgtg
     9661 aacatctaca cattcccgta ccggaaaaat tatgcgtgat tggcatcgac aacgaagaac
     9721 tgacccgcta tctgtcgcgt gtcgcccttt cttcggtcgc tcagggcgcg cggcaaatgg
     9781 gctatcaggc ggcaaaactg ttgcatcgat tattagataa agaagaaatg ccgctacagc
     9841 gaattttggt cccaccagtt cgcgtcattg aacggcgctc aacagattat cgctcgctga
     9901 ccgatcccgc cgttattcag gccatgcatt acattcgtaa tcacgcctgt aaagggatta
     9961 aagtggatca ggtactggat gcggtcggga tctcgcgctc caatcttgag aagcgtttta
    10021 aagaagaggt gggtgaaacc atccatgcca tgattcatgc cgagaagctg gagaaagcgc
    10081 gcagtctgct gatttcaacc accttgtcga tcaatgagat atcgcaaatg tgcggttatc
    10141 catcgctgca atatttctac tctgttttta aaaaagcata tgacacaacg ccaaaagagt
    10201 atcgcgatgt aaatcgcgag gtcatgttgt agcgatgatg agaattgtcg gcgtcacatc
    10261 agggaactca gcacagccac ctgatgcgag caaaagcctt atccgaccga caaatcagat
    10321 aaggctcaag gaaatgcaat tacatatgcg ccgcgattaa ccgttggtta tcctggtaca
    10381 ttgcgaacag gtagttatta taactcttcc ctttggtcga gtagcccttc agcttgtgaa
    10441 tcatcgctgt ggcagtcact tcctgatccg ctttacgcag ctgcgcacgc gatttacgga
    10501 acgaagagta agccgggtgc gtattcaggt tagtgacata ggcgctcacc gattctttga
    10561 cagaactaaa ctgtgagtac cctttcactt tacccggcgc attggtacaa cgtcctttca
    10621 tgcatttcat gccgaacaga ttgttattgt tgcgcgccag cttcgacgtt ccccaaccgc
    10681 tttctgctgc agccatcgtc gccaccatac tggtagggat aatgtctacg cgttcaagca
    10741 aggtattcca cgggattttt cgcgtattac cagaccactt caccttgtag cgtttggcga
    10801 tgtctttcag acgcgcacgc tcagcaggtg accattggcc ctgatactgt tttgaaatca
    10861 gccagttacg ttctgcagta atggctgcat tttggctggt aatgtaaggc attacggtcc
    10921 ggagaaacgc ctttttcctt ggtgttccgg aagggtattt tcgcaaatca ggaagtgaac
    10981 gactctttgc actattgcga gaatactctt gtttactgct tacctgttta ttactagctt
    11041 tagttaagtg ggacttttga ctcgctgttg ttgtgtgcgt cttcgctaac acctcactcg
    11101 aaaacaccag agtgagtaac ataagaatca tcgccccata tcgtcgtatg ggagtcaaaa
    11161 tcatcaggtc tcctggtcgg atttaatcat tccaacacct tatatttttc acaaatttga
    11221 gagttgaatc tcaaatcata tcaaaaatag ctgtcaagag caccccaagg aatagtccaa
    11281 atctgaaact atgtcacgag ttaacgattc agattggcgc taaatcgcag aaaatgtggg
    11341 gtttatcgca aaattcagcc gttttttgcg cgcgatcgct cacccttgct tctcatcctg
    11401 tggacttacc gctcagggat gagttttgtc tggcttatcg ctggcaaact gtctgaaatc
    11461 gcagcaataa ggactcatcc accatgaaac tcgcctcctg ttttctgaca ctccttcctg
    11521 gcttcgccgt tgccgccagc tggacttctc cggggttccc tgcctttagc gaacagggaa
    11581 cgggaacatt tgtcagccac gcgcagttgc ccaaaggtac gcgtccactc acgctaaatt
    11641 ttgaccaaca gtgctggcag cctgcagatg cgataaaact caatcagatg ctttccctgc
    11701 aaccttgtag caacacgccg cctcaatggc gattgttcag ggacggcaaa tatacgctgc
    11761 aaatagacac ccgctccggt acgccaacat tgatgatttc catccagaac gccgccgaac
    11821 cggtagcaaa cctggtccgt gaatgcccga aatgggatgg attaccgctc acgctggatg
    11881 tcagcgccac tttcccggaa ggagccgccg tacgggatta ttacagccag caaattgcga
    11941 tagtgaagaa cggtcaaata acgttacaac ccgctgctac cagcaacggt ttactcctgc
    12001 tggaacgggc agaaactgac acatccgccc ctttcgactg gcataacgcc acggtttact
    12061 ttgtgctgac agatcgtttc gaaaacggcg atcccagtaa tgaccagagt tacggacgtc
    12121 ataaagacgg tatggcggaa attggcactt ttcacggcgg cgatttacgc ggcctgatca
    12181 acaaactgga ttacctccag cagttgggcg ttaatgcttt atggataagc gccccatttg
    12241 agcaaattca cggctgggtc ggcggcggta caaaaggcga tttcccgcat tatgcctacc
    12301 acggttatta cacacaggac tggacgaatc ttgatgccaa tatgggcaac gaagccgatc
    12361 tacggacgct ggttgatagc gcacatcagc gcggtattcg tattctcttt gatgtcgtga
    12421 tgaaccacac cggctatgcc acgctggcgg atatgcagga gtatcagttt ggcgcgttat
    12481 atctttctgg tgacgaagtg aaaaaaacgc tgggtgaacg ctggagcgac tggaaacctg
    12541 ccgccgggca aacctggcat agctttaacg attacattaa tttcagcgac aaaacaggct
    12601 gggataaatg gtggggaaaa aactggatca gaacggatat cggcgattac gacaatcctg
    12661 gattcgacga tctcactatg tcgctggcct ttttgccgga tatcaaaacc gaatcaacta
    12721 ccgcttctgg tctgccggtg ttctataaaa acaaaacgga tactcacgct aaagtcatcg
    12781 aaggctttac acctcgcgat tacttaaccc actggttaag tcagtgggtc cgcgactatg
    12841 ggattgatgg ttttcgggtc gataccgcca aacatgttga gttgcccgcc tggcagcaac
    12901 tgaaaaccga agccagctcc gcgcttcgcg aatggaaaaa aactaacccc gacaaagcat
    12961 tagatgacaa acctttctgg atgaccggtg aagcctgggg ccacggcgtg atgcaaagtg
    13021 actactatcg ccacggcttc gatgcgatga tcaatttcga ttatcaggag caggcggcga
    13081 aagcagtcga ttgtctggcg cagatggata cgacctggca gcaaatggcg gagaaattgc
    13141 agggtttcaa cgtgttgagc tacctctcgt cgcatgatac ccgtctgttc cgtgaagggg
    13201 gcgacaaagc agcagagtta ttactattag cgccaggcgc ggtacaaatc ttttatggcg
    13261 atgaatcctc gcgtccgttc ggtcctacag gttctgatcc gctgcaaggt acacgttcgg
    13321 atatgaactg gcaggatgtt agcggtaaat ctgccgccaa cgtcgcgcac tggcagaaaa
    13381 tcagccagtt ccgcgcccgc catcccgcaa ttggcgcggg caaacaaacg acactttcgc
    13441 tgaagcaggg ctacggcttt gttcgtgagc atggcgacga taaagtgctg gtcatctggg
    13501 ctgggcaaca gtgacttttc cggcttcccg ttcatcagta cctcgggaag ccgccaacca
    13561 ggataaaatg tcagccctaa tcagcgttgc aggataaagc accgctcact cttcaacaga
    13621 ccgatttgca ccccagcaaa tgtagcgtta ttgttacctt ccttgctaca gagttcgaca
    13681 gatatcccgc tatgacattc tccctttttg gtgacaaatt tacccgccac tccggcatta
    13741 cgctgctgat ggaagatctg aacgacggtt tacgcacgcc tggcgcgatt atgctcggcg
    13801 gcggtaatcc ggcgcagatc ccggaaatgc aggactactt ccagacgcta ctgaccgaca
    13861 tgctggaaag tggcaaagcg actgatgcac tgtgtaacta cgacggtcca caggggaaaa
    13921 cggagctact cacactgctt gccggaatgc tgcgcgagaa gttgggttgg gatatcgaac
    13981 cacagaatat tgcactaaca aacggcagcc agagcgcgtt tttctactta tttaacctgt
    14041 ttgccggacg ccgtgccgat ggtcgggtca aaaaagtgct gttcccgctg gcaccggaat
    14101 acattggcta cgctgacgcc ggactggaag aagatctgtt tgtctctgcg cgtccgaata
    14161 ttgaactgct gccggaaggc cagtttaaat accacgtcga ttttgagcat ctgcatattg
    14221 gcgaagaaac cgggatgatt tgcgtctccc ggccgacgaa tccaacaggc aatgtgatta
    14281 ctgacgaaga gttgctgaag cttgacgcgc tggcgaatca acacggcatt ccgctggtga
    14341 ttgataacgc ttatggcgtc ccgttcccgg gtatcatctt cagtgaagcg cgcccgctat
    14401 ggaatccgaa tatcgtgctg tgcatgagtc tttccaagct ggggctgcct ggctcccgct
    14461 gcggcattat catcgccaat gaaaaaatca tcactgccat caccaatatg aacggcatta
    14521 tcagcctggc acctggcggt attggtccgg cgatgatgtg tgaaatgatt aagcgtaacg
    14581 atctgctgcg cctgtctgaa acagtcatca aaccgtttta ctaccagcgt gttcaggaaa
    14641 ctatcgccat cattcgccgc tatttaccgg aaaatcgctg cctgattcat aaaccggaag
    14701 gagccatttt cctctggcta tggtttaagg atttgcccat tacgaccgag cagctctatc
    14761 agcgcctgaa agcacgcggc gtgctgatgg tgccggggca caacgtcttc ccgggactgg
    14821 ataaaccgtg gccgcatacg catcaatgta tgcgcatgaa ctacgtacca gagccggaga
    14881 aaattgaggc gggggtgaag attctggcgg aagagataga aagagcctgg gctgaaagtc
    14941 actaaaaatt gcctgatgcg ctacgcttat caggcctaca tgatttctgc aatatattga
    15001 atttggaaga atttgtaggc cggataaggc gtttacgccg catctggcat aaacaaagcg
    15061 cactattccg gcgttgagaa acgccggaaa acgtttcaaa caggctgctg ccgtagccga
    15121 tgccgctgca ctttctcgac atccatgcac tgcaacgcct ttgtcgggca ggcttcaaca
    15181 caggccggcc ccgtctcccg atgccagcac agatcgcact taatcgccct cgcctttttg
    15241 cgcgaagaaa cgacctccat cgcaccaaac gggcaagcca gcatacagct tttacagcca
    15301 atgcaacgtg tttgttcaac gaaaatatgc ccatgttcgc ggcttatcgc gtcaacaggg
    15361 cagacattcg cgcacggtgc atcttcacac tgatgacagg ctactgccgt ggtccagctg
    15421 tggtctttaa tgacacgaat acgggaaata aactcgtctg gtgacaacgc agcgcaatcc
    15481 tgattctcct gatgcgacac tgcgcaagcc acttcacagg tacggcaacc gatacatttc
    15541 gtcgcatccg caataataaa ccggttcatg atcactcctt tgcactaaaa cagcagagtg
    15601 ccagagagcg cgtccctact gctttgatcc aacgaaggaa atgttaaagt tttggcactt
    15661 gtccaaagcg ttatgtgatc gcgcccaggt catccctgac agtaaatccc agttcattag
    15721 aaatagcctg cgcggtttca cgcagtggtt tcaggagatt tttctctccc acctgtttca
    15781 gacgtgatgt cgaaagcgaa atcgacacgg cgtacggcac ccgcccatga atatcaaaca
    15841 ccggaacagc aatacaggag acgccgagtt cgttttcttc tctgtccatc gccgctccgc
    15901 tttcacgaat gtgcgccagt tcgtcgaaca tcgcgggcag ctcggtaatg gtattgcggg
    15961 ttaacggctg gatctcatgc tgatggtttt cccagtatga cttcacgtag tccgggtgac
    16021 caaacgccat gtagatcttg cccattgcgg aacagtagag cggcatatgc tggccaatat
    16081 aggcacgggt tcgcagcatc ccggttgtgg gttccagctt ataaatcaaa atagcgtgat
    16141 cgtcttcgcg gctggagaag ttaatggttt caccagtggc gatgttcagt gcctcaagat
    16201 gcggagcggc gatatgaatg atattcagcg aagacagcgc cttctgcccg acggcaataa
    16261 atttggtagt caggcggtaa ctccctgcgg cgggcgcggt ggtcacatag ccgcaggact
    16321 gtaatccctg caataagcga tggacggtac tcttatttaa accagccagc tccgaaagat
    16381 gcgccaacgg acaaccgttt ggatagttgc tcaaaatctc aatcagcatc aacccgcgaa
    16441 acagactctg gcttccggct ggacgctctt tttcctgcgc catctcgttc tctttttttc
    16501 ccatcacttc tttccccatt ttgtcgcgtc ctgatggtag cgcaaagtgt gccgtagttc
    16561 acgatctcga cagataattt ataaccactt gatttttatg tcttttgaaa ttcatcaatc
    16621 agattgcttt gttaaaaagt gatcgatata tttgaaatca agtttcgcat attgaaattt
    16681 taagccaaaa aagcgatcaa aaaaacaagg aagcctggga tgaaagtgac atttgagcag
    16741 ttaaaagcag cctttaatcg ggccttaatt tcacgcggcg ttgacagcga aacggctgac
    16801 gcctgtgcag agatgttcgc ccgcaccacc gaatccggcg tttattctca cggcgttaat
    16861 cgtttccctc gtttcattca acaactggaa aacggcgata tcattcctga tgcccaaccc
    16921 aaacgtataa ccagcctcgg cgcaattgaa cagtgggatg cccagcgttc gatcggcaac
    16981 ctgacggcga aaaagatgat ggatcgcgcc attgaactgg ctgccgatca cggtattggt
    17041 ctggtggcac ttcgtaatgc caaccactgg atgcgcggcg gcagctacgg ctggcaggcg
    17101 gcggaaaaag gctatattgg catttgctgg accaactcca tcgccgtaat gccgccgtgg
    17161 ggcgcaaaag agtgtcgcat tggcactaac ccgctgatcg tcgccattcc ttccacgccg
    17221 atcaccatgg tcgatatgtc gatgtcgatg ttctcttacg gcatgttaga agttaaccgc
    17281 ctggcgggcc gtcagctccc ggtcgatggt ggctttgatg atgagggcaa tttgaccaaa
    17341 gaacctggcg ttatcgagaa gaatcgccgc attttgccga tgggctactg gaaaggttct
    17401 ggcatgtcga ttgtgctgga tatgatcgct actctccttt ccgacggcgc atcggttgcc
    17461 gaagtcaccc aggacaacag cgacgaatac ggcatttcac aaatttttat tgccattgaa
    17521 gtggacaagc ttatcgacgg tcccacccgc gatgccaagc tgcaacgcat catggattac
    17581 gttactaccg ctgaacgcgc tgacgaaaac caggccatcc gcttacccgg ccacgaattt
    17641 actaccctgc tggccgaaaa ccgccgtaac ggcatcactg ttgatgacag cgtgtgggcc
    17701 aaaatccagg cgttatgagg agataagtca tgatttttgg acatatcgcg cagcccaatc
    17761 cgtgccgttt gcccgccgcc attgaaaagg cgctcgattt tctgcgcgct accaacttca
    17821 acgtcctgga gccgggcgtt gtcgaaatcg acggcaagaa tatttatgcg caaattatcg
    17881 atttaaccac acgcgaagcg gtggaaaacc gtccggaagt gcatcgtcgg tatatcgata
    17941 ttcagtttct ggcgtggggt caagagaaaa tcggcattgc tattgataca ggaaataata
    18001 aagtcagcga atcactatta gagcagcgcg atattatttt ttatcatgtc agtgaacatg
    18061 aatcatttat agaaatgata ccgggcagct acgctatttt cttcccgcag gatgttcacc
    18121 gaccaggttg tattctgcaa acaggcctct gagatacgaa aaattgtggt taaagtcgca
    18181 ttaacagcgc tgaattaatc aaataatcgt ctacgccaga acgactgggt aatgttattg
    18241 ctctaactat attaataatt taggttgctg cacatgcaac ttgaattatg acagacatag
    18301 cctcaaggaa tagctatgaa aaaaatactt gaagtaatac tggcgattaa tctcgccgta
    18361 ctttcctgta ttgtgtttat aaacattatt ttaagatatg gttttcagac aagcatttta
    18421 tctgtagatg aattgtcacg ttatttattt gtctggttaa catttattgg cgcgattgtg
    18481 gcttttatgg ataacgccca cgttcaggtc acctttctgg tggagaaact ctcccccgca
    18541 tggcaacgac gagtcgctct ggttacccat tccttaatct tatttatttg tggcgcactg
    18601 gcctggggcg caacactaaa aacaattcag gactggagcg attattcacc gatcctcggt
    18661 ttgcccatcg gcctgatgta tgccgcttgt ctgcccacca gcctcgttat cgcattcttt
    18721 gaactgcgtc atttatatca actcatcacg cgtagcaatt ctctaacgtc gccaccgcaa
    18781 ggagcttaat catggctgtg ctgatttttc tgggctgtct gttgggtggt attgctatcg
    18841 gtttgcctat tgcctgggca ctgttgttgt gcggggcggc gttaatgttc tggctggaca
    18901 tgtttgatgt ccagatcatg gcacaaacgc tggtgaacgg agccgatagc ttctccctgc
    18961 tggcgattcc gttctttgtg ctggcgggtg aaatcatgaa tgcgggcggc ttgtcaaaac
    19021 gcattgttga cctgccgatg aaactggtgg ggcataaacc aggcgggctg ggctacgtcg
    19081 gcgtgctggc agctatgatt atggcgagcc tttctggttc tgccgttgcg gataccgccg
    19141 ccgttgccgc tctactggtg ccgatgatgc gcagcgccaa ctacccggtc aaccgggcgg
    19201 cggggctgat tgcctctggc ggcattatcg cgccaattat tcccccttcc attccgttta
    19261 ttatcttcgg cgtttccagc ggattatcca tcagtaagct gtttatggcg ggcattgccc
    19321 ctgacatgat gatgggcgca acgctgatgc ttacctggtg gtggcaggcc agtcgcctta
    19381 atttaccccg ccagcaaaaa gcaacgatgc aggaaatctg gcactctttt gtctccggta
    19441 tctgggctct gtttcttcca gtcatcatta ttggcgggtt tcgctccggt ctgtttaccc
    19501 ctaccgaagc cggggccgtt gctgctttct atgcgctgtt tgtcgccaca gttatttacc
    19561 gtgaaatgac cttcgccaca ctttggcatg tactcattgg cgcggcgaaa accacctcag
    19621 tggttatgtt tctggtggcc tcggcacaag tttccacctg gctgattacc attgctgaac
    19681 tgccgatgat ggtttctgat ttactgcaac cgttggtcga ttcaccgcgt ctgctgttta
    19741 tcgtcattat ggtggctatc ctgattgtcg gca
//
LOCUS       DAJVUJ010000071        19596 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.071, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000071 DAJVUJ010000000
VERSION     DAJVUJ010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19596
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.071"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(190..531)
                     /locus_tag="OQQ48_002257"
     CDS             complement(190..531)
                     /locus_tag="OQQ48_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HCS1953014.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVEGLKL
                     IESGDWLDRDK"
     gene            complement(534..4313)
                     /gene="tamB"
                     /locus_tag="OQQ48_002258"
     CDS             complement(534..4313)
                     /gene="tamB"
                     /locus_tag="OQQ48_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HCS1953015.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPDEKPLGETLKDLFSR
                     PVLPEMTDLHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVTQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTLVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDFAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVLVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(4310..6043)
                     /gene="tamA"
                     /locus_tag="OQQ48_002259"
     CDS             complement(4310..6043)
                     /gene="tamA"
                     /locus_tag="OQQ48_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HCS1953016.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSCGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            6249..6887
                     /gene="msrA"
                     /locus_tag="OQQ48_002260"
     CDS             6249..6887
                     /gene="msrA"
                     /locus_tag="OQQ48_002260"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCS1953017.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNEPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            7210..8553
                     /locus_tag="OQQ48_002261"
     CDS             7210..8553
                     /locus_tag="OQQ48_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCS1953018.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(8615..8821)
                     /locus_tag="OQQ48_002262"
     CDS             complement(8615..8821)
                     /locus_tag="OQQ48_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HCS1953019.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            9146..9700
                     /locus_tag="OQQ48_002263"
     CDS             9146..9700
                     /locus_tag="OQQ48_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCS1953020.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLQKLLK"
     gene            complement(9790..10701)
                     /locus_tag="OQQ48_002264"
     CDS             complement(9790..10701)
                     /locus_tag="OQQ48_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1953021.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSHTKTHSNNFFNNIQHAFKKKDWV
                     SNYDSLLTLWEFFRCATQIDKDSYQVLSSKNETVKAMDKFLISFSLKDNGAEYTMTLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYAWDILQKKQSDIPKRIKGYIHQ
                     AIGDVSLGYALLEDIVSKLKRGKFELQIPGGGIKECDGWYIYEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAVDS
                     DITEEEICEKYKSTIEAFKKEQLALPV"
     gene            complement(10913..11653)
                     /gene="cysQ"
                     /locus_tag="OQQ48_002265"
     CDS             complement(10913..11653)
                     /gene="cysQ"
                     /locus_tag="OQQ48_002265"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HCS1953022.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            11843..13786
                     /gene="cpdB"
                     /locus_tag="OQQ48_002266"
     CDS             11843..13786
                     /gene="cpdB"
                     /locus_tag="OQQ48_002266"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HCS1953023.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEEADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDRHIAFASPDE
                     NRSVLTAWIADESKRAGEIHPAADNNWRLAPIAGDKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(13915..14295)
                     /locus_tag="OQQ48_002267"
     CDS             complement(13915..14295)
                     /locus_tag="OQQ48_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCS1953024.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGGSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDEHAA"
     gene            14384..15244
                     /gene="qorB"
                     /locus_tag="OQQ48_002268"
     CDS             14384..15244
                     /gene="qorB"
                     /locus_tag="OQQ48_002268"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCS1953025.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            15352..16317
                     /locus_tag="OQQ48_002269"
     CDS             15352..16317
                     /locus_tag="OQQ48_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCS1953026.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALVCIGIGLACVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNTQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLIRQQMPPLMTLSGIALLVVGVVIVVRAKPEKPLTESVSES"
     gene            16425..17087
                     /gene="ytfE"
                     /locus_tag="OQQ48_002270"
     CDS             16425..17087
                     /gene="ytfE"
                     /locus_tag="OQQ48_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HCS1953027.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYNMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(17132..18544)
                     /gene="cycA"
                     /locus_tag="OQQ48_002271"
                     /gene_synonym="dagA"
     CDS             complement(17132..18544)
                     /gene="cycA"
                     /locus_tag="OQQ48_002271"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HCS1953028.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFLG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWTGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(18853..19473)
                     /gene="fklB"
                     /locus_tag="OQQ48_002272"
     CDS             complement(18853..19473)
                     /gene="fklB"
                     /locus_tag="OQQ48_002272"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCS1953029.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
BASE COUNT         4798 a         5190 c         4774 g         4834 t
ORIGIN      
        1 catactcacc gttcaaagcg tgcccccgtt ttacccctgc acatttgtga acgccagcag
       61 agttttttat gcgattgcag atatggaaag cggctcggag agaaaagcaa aagatggaaa
      121 agttacaaaa aaaaatcgcg agatacatcg cataaaacaa cgccacccga aggtggcgtg
      181 cttttattat tacttatccc tgtctaacca gtcgccgctt tcaattagct ttaatccctc
      241 gacgggtcgt tgataaacgt acatccatgc actcccgtac ggcgtctgaa tcaactggcg
      301 cgcgtattca ccgcccctgg tgcgcaaggc atcaagttcg gccagcgtgg cgttgtcaat
      361 acgataaact tcaccgtgta ccgttccgtt ccccggaact gcgcctggat agtggcccag
      421 gctatacaac tggtagttat cgatactgaa atcgcccagt aactgggcat tggtcatcca
      481 gtgactgttg ccttgtttgt ggcgtaaact gccgtagaca aatattcgca ttgctaaaac
      541 tcgaactgat agagcaaatc cagtgcctgg tctacaccag acacggcttc cagatatagc
      601 ttaggcatca ggcgataacg taacgtgagt gttgctatag agtcaaatat acccacgccg
      661 tatttcactt gcaggcctgg caatacatag ccgctgacca ctacctggga ggagtcgcct
      721 actccctggg tgtcgagcgc taaattgctt acgccaaacg tctcgccgat tttacccaca
      781 atctggccac tttgcgcaac ccccagacca atcagcatcg aggtcattgc cgcactgtcg
      841 ctctgatcgc tctccagccc ttgtccacgt agcaaataag acaatgcagc ttgttgcgac
      901 atcgccgggt cagagaagat ctccgctttc ggttcgtccg ccagaccagt gacgcgaact
      961 ccggcgatta cgtcgtcttc tgtagcatcc gggttacgaa tagcttcaat attaagatac
     1021 ggttgatctg gcggaccaga gaacagtagc tcacccttac gcacaatcag atcctgacca
     1081 taggcatgga agcgcccttc agggatgttg atctgcccgt tcaggcccag cccttgtttg
     1141 tcctgaacga cgttaagatc gcccgtcagc cgcgctttca ggccaaaggc gtcaatgcgc
     1201 acattgttgc caacgtggac aatcaggtta ctgttaatcg gaatcgacgc cgttttcggc
     1261 tcttccggtt gcaggttatc gttaagcatc accacatcgc tggagacgcc tactgcgctt
     1321 tccggcagat cgtgcaccac gatgcgcgcc cacgggacat ccacgcgacc atcgagggta
     1381 aacaggtttg gtgtagcctc gaatacaaca tctggcgata catccattcg taccatcggc
     1441 ggcacggtga tccgcacttt actgcctttc gccgttactc gtgcccgcca gttttcaatt
     1501 tggctccagt cggcatcacc gttcaggtag atttcaccct gctgggtccg tactgtaccg
     1561 gcaagcgtcg agcgcatacc gttaaagttg accgcaagct ggctcggctg catatcaaac
     1621 ggcataaagt tgccgtcgat atccacaccc gtaacctgaa gctgaccaaa caactgcggg
     1681 ctttgcacat caccacccag acgcaagttg gcactcacca tccccgctgc tttttcccca
     1741 cgagtaaaga tggggtttat catcgcaagg ttgaagttac ggatattgac gttgccacca
     1801 agattacggc ggccttgcgg atcggtcacc agcacctgtc catcaaactg gccgttattg
     1861 gtcaggcgga tggtccagcc caattcggca cggttgttac gcaattccgc cgtcagattc
     1921 agtgtctgaa acgccaccgg cagcgccgca tcgttgacgg tttgcgttac ctgcacgtta
     1981 cgccccgaaa gggtgatact gccctgcggc agcccctctt tcgtggtgtc ccaggcaaca
     2041 tccgctttac ccgtgaagat accgctggcc tgagtggttt ctggcataaa cggtttcagc
     2101 atggcgaagt cgaagcggtt gagattcacc accgcacgcc cttcggcccc cgcatcgata
     2161 gtttgcggca cgcacagttc cgcattcggg ttaagccagc agtgtggccc gatgctgatt
     2221 ttttgctcct tattgcggta atccagcgca atatcgcggg tcagcgacca cgggccgacc
     2281 agcgtctgga agcgggtatt gctaagagtt cccttccagc gttcttcttt gcgatcaaaa
     2341 cttcctgcca gattaagctg cccggagaca ggctcgccct gaatccgcaa ctgtagctcg
     2401 tgctgctttt cgctgccttt ggcattcagg gtgacgaggt tgatatttac atccggctgc
     2461 gaaatttgct caacgcgtac gtcgagtttc ccggcgatct gatcggtgga tttaatgtcg
     2521 ccttccacgc gaacctgcgt cacggaaagt tcctgccagc gcaggccgcg cgcggtgata
     2581 tctgccagta gttgtggcgc ttccaccgtg ccgcgtactt tcaccagccc tttcgctgta
     2641 ccgccaagcc ccggcagcgc gttatccagc cccggcgcgt tgatggtggc atcaagattg
     2701 agatctttta cccccagctc gcctttcact tcggcactgt ttggccccag ttccagatga
     2761 agcccaggga tcatccactg catataactg ttgcctttca gcgtgccgtc aacgttcact
     2821 ttgttctgtt tgacgttacc ggtcagcttc agttctggca cctccatctg ccaggtgccg
     2881 ccgtacaggc taccgcgggt tttaatcaag ccattgagtt tcgacggcca ctccgggaac
     2941 tctttggcgg tgttaatgcc attaagcgtt agctcaccgc gccaactaat ggcctgctgc
     3001 cagtcgagca acgccttgag ttcagttttc ccttccagcg ccgcgacggt gagtttgtcg
     3061 agattgacct gctgttcatt acctttggca tcaagggtaa tggtggctgg cgggatctcc
     3121 tgtcccttca ctgccgtacg catagagagc gtgtaatcgg tcattttgcc ggtaagtttc
     3181 agtttcagat catccgcctg atactgcttc tcaccagtga acggccagta aagctgtttg
     3241 ctgttcactt ccacgttgag cggcaatccg gcttccgcca gtcgcgtctg ggcgcgtaaa
     3301 tccatatcca ccggaccgga aagattaacg ccaatctcca gctgttcgcg cagcgcgccg
     3361 cccactttta gcttcacttt ttcacctttc aacggctcca cgttcagtgt gctgttgagg
     3421 gtgatatcca ccggccagtt gtctgacagc tgcgccgtgc cgctggcgtt gacgatcccc
     3481 tggttggaat cgatatccag ggcgtccagt ttagtattgc cgtcaatgct gctcactttc
     3541 agcagcatgg tgcgcacggt gatgtccgtg tcgcccgtca cgcgtaactg ctcgccttta
     3601 aactcttcaa tgttcaggtt aagcggcaaa tgcaagtcgg tcatttccgg caataccggg
     3661 cgagaaaaga gatctttcag cgtttcgccg agcggctttt catccggctg cggattttca
     3721 attttcggtt cgaccacttc ttcctgcgcc acttccgcca ctttcggcag agcaatcagc
     3781 aggcctttca gcgacgtcgg tttcagggtc agggttttct cctgccagtt caggccggag
     3841 gtgaagtcca tcaccgacac cgtggtgtca tcaatcttga tgttgacgtt gtccagcgcc
     3901 acccgtgtca gggtgatggg atacggcgtg gagagatcca gcggaccgct atcttcctct
     3961 tcttcaacct gttcagaagg aggcattttt ttactgtcga tgttgacctg aatgtctttc
     4021 agcgccaggt cattaataca aacgctactg ttccacaggc actcaagacc gaccgccaga
     4081 tgcagattac ccgcttttac cgccacgcct ggctgctcat aacgaacgtc agacaaggtg
     4141 agatcgcgcc agccgccggt gaccttgcca atatccagtc ctggcaccca gcgatccgcc
     4201 gctttaaata caagatgcag gccgctggtg gtgcccacca gaaacgccac cgatcccagc
     4261 aacagtaaga taacgataac cacgccgagg ctgatttttt tccataaact cataattctg
     4321 gccccagacc gatgtaaaac tgtaacccgt gttcgtcttt atccgcgacc ggtacggcaa
     4381 aatcgagttt gattggcccg accggcgatt cccagcgcac gccgaccccg gtaccggttt
     4441 taaagtcgct gcggcgaata tcgcttaccg cttcgccact atcgacaaac accgcgcccc
     4501 accattttcc ggtcacgttg tactggtatt ccagcgatcc ggttatcaac ttcgaggccc
     4561 ctttcaggtc accgttggcg tatttcgggg cgatagattt gtatttgtag ccgcgaatac
     4621 tgcgatcgcc cccggcgaaa aaacgcagat ccggcggtac tttgtcgaaa tcaccggttt
     4681 caatccagcc cagcgtgccg cgtgtaacga aacgatggcg atcgtacagt gtgcggatcc
     4741 agacgttctg cgcctggaaa acggagaaat cgacatctga gccccaggcc gtgttggagt
     4801 agtcgataga gtagcgttgc gagtcgcccc aggttggcat caggccacca caagaacgcg
     4861 tgcggctaat catcacccca ggataaaaca gcatcgtggt gttggtaatt tcaccctgag
     4921 taaagtggtc gagactccag cgcaggttaa tggcacgctg ccaaccgctg gagagatccc
     4981 agtagcgaga agccaccagc gtagtggagt cagattcggt atcgttcagg tcagtgcgct
     5041 taaaaccgcc ctgcaccaaa taatattgtt ccagtggatt cttcagcagc ggcattttat
     5101 agctgaagtc gagggtctgt tccggcgcgg aaatactggt actggtggtc aaactgtgac
     5161 cgtatgagtt catccacggc tttttccacg tcgctttcac gcgcggtccc acgtccgtag
     5221 agtaaccgac cccggtttcg atggtgtttt ctgttcgcgg cgaaaccacg cccgtcaatg
     5281 gtaatacttt cgtttcgcgc gctttatcaa attgtggagc caccaccacc gagttaaacc
     5341 agccggtagc agaaagtcgg cggttcagtt ccgccagatc tttcgattca tactcatcgc
     5401 cctctttaaa cggcaccaga ttttgcaggt attcatcgcg gatttgtgat ccttcaaagg
     5461 tcacatgccc aaagcggtaa cgttcgccac tgttataatc aatatcccag aaggctttat
     5521 gcaggccgag cgcaatgccc agctgcgctt tggtaaattc gctatcgaaa taacctttac
     5581 gcaacgcaat actggttaag gactttttga aattttcata atcgccctgg ttcagtaccg
     5641 taccaatagc cgggcgagta tcgagcaatt tcaaatagtc tttatcggtc cgcgcgccgc
     5701 cgcgcaatac cacatcggtg ccgccaatta acaccggcac acctggcgtg actttggcga
     5761 tcaatacctg ccgccctttc tttggcggtg gacggagatc aaattcaatg gtcggctggt
     5821 aataacccag cgctttcaga ccttcacgga tggcatcatc gacgcgtgcg cgaaagcggc
     5881 ggtctggcgt cacttcatca ctttcaatcg tagaaagctg cgcacgaacg ttcttttcca
     5941 gctgtcccga taacccctcg acctgtagac ggacgttcgc ggcgacggca gatccgctta
     6001 agcagagtaa gcttacacag cataactgtc ggatatagcg cacattttct cctgaatatc
     6061 cttttttcct gcccctggaa cgccgttaaa cggcttaaca aaaatccagt aatatggatt
     6121 aaaaagcaga ctaaacccca aatatttctt atgttttact ttagacctat tcacggtggt
     6181 tattgtgtgc aaatacgcct cttgttacaa ccttaacccc aatgaccgat tttcgggaga
     6241 gcgacaccat gagtttattt gataaaaagc atctggtttc ccccgccgat gccctgcctg
     6301 gacgtaacac cccgatgccc gtagccacgc tgcatgcggt caacggtcac tcaatgacca
     6361 atgaacctga cggaatggag attgccattt ttgcgatggg ttgtttctgg ggtgtggagc
     6421 gtctgttctg gcagttaccc ggcgtttaca gcaccgccgc aggctatacc ggcggctata
     6481 cgccaaatcc gacttatcgg gaagtgtgct ccggtgatac gggtcatgcc gaagcggtac
     6541 gcattgttta cgatccttcc gtcatcagct atgagcagtt gctacaggta ttttgggaga
     6601 atcacgatcc cgcccaggga atgcgtcagg gcaatgacca cggcacgcag tatcgttcag
     6661 cgatttatcc gctgacccca gaacaggatg ccgcagctcg cgccagtctg gaacgttttc
     6721 aggcggcgat gcttgccgcc gatgacgatc gtcacatcac cacggaaatc gctaacgcca
     6781 caccgtttta ttatgccgaa gatgaccacc agcaatatct gcataaaaac ccatatggtt
     6841 actgtggaat tggcggaatt ggcgtctgtc tgccgccgga agcatagcgt tacgggtaca
     6901 aatgtagttt gttgataaag tgctctttgt ctatgcctga tgcggcttaa acgacttacc
     6961 aggcctacaa aatcgtccaa attcaatata ttgcaaggac tgcgtaggcc tgatcggcat
     7021 agcgcatcag gcagtattgc atttatcagc agtctgaatt ttaaccctct ggcgacttta
     7081 cagtacctta cgctatacta gccactgaaa attccggttc actttcttcg aatcggcttt
     7141 caatgtgtat ttcacacaaa ttaatcaact ttcccttccg aggatctggc ctgaaaggtc
     7201 ggataagata tgttaaacag tattttagtc atactctgct tgatcgctgt aagtgcgttc
     7261 ttctcgatgt ccgagatctc gcttgccgcc tcacgcaaaa tcaaacttaa actgctggct
     7321 gatgaaggca atataaatgc ccaacgcgtt ctgaatatgc aggaaaatcc cggcatgttc
     7381 tttaccgtgg tccaaatcgg tctgaacgca gtggcgattc tcggcggtat cgtcggtgat
     7441 gcggcatttt ctccagcttt tcacagcctg ttctcccgct atatgtcggc agagctctct
     7501 gagcaactga gctttattct ctctttctcg ttagtgactg gcatgtttat cctgtttgcg
     7561 gatttaaccc cgaaacgcat cggtatgatt gcgccagaag cggtggcttt gcgtatcatc
     7621 aacccgatgc gcttctgcct gtacgtttgc accccgctgg tgtggttctt caacggcctg
     7681 gcgaacatga tcttccgtat tttcaaactg ccaatggtac gtaaagatga catcacttct
     7741 gatgacatct acgcggtagt ggaagccggt gcgctggcgg gcgtgttacg taaacaggaa
     7801 cacgagctga ttgaaaacgt ctttgagctg gaatcccgta ccgttccgtc ttcaatgaca
     7861 ccgcgtgaaa acgtgatttg gtttgatctc cacgaagatg agcaaagcct gaagaataag
     7921 gtggcggaac atccgcactc taagttcctc gtctgtaatg aagatattga ccacatcatc
     7981 ggttatgtcg attctaaaga cctgctgaac cgcgtgctgg ctaaccaaag cctggcactg
     8041 aacagcggcg tacaaattcg caacacgctg attgtgccgg atacgttaac cctttcagag
     8101 gcgttggaaa gttttaaaac cgcaggtgaa gacttcgcgg tgatcatgaa cgagtacgcg
     8161 ctggtggtgg ggatcatcac cctcaacgac gtgatgacca cgctgatggg cgatctggtc
     8221 ggtcaggggc tggaagagca gattgtcgcc cgtgatgaga actcatggct gattgacggc
     8281 ggcaccccaa ttgacgacgt catgcgcgtg ctggatattg acgagttccc gcagtcgggc
     8341 aactacgaaa ccatcggcgg ctttatgatg tttatgctgc gtaagatccc gaaacgcacc
     8401 gattcggtga aattcgccgg ctacaaattt gaagtggtgg atatcgataa ctaccgcatc
     8461 gaccagctgc tggtgacccg gatcgacagc aaggccaccg ccctttcgcc aaaactgcct
     8521 gacgctaaag ataaagaaga aagcgtcgcg taacccccag aaacatcaac ggctcctgaa
     8581 tcaggagccg tcttattact gcatagcact ttggttaagc catctctgtt tgcagacgca
     8641 taacctgacg gttaacttcg gacatcactg acaaatgcag cttatccttc acttttggga
     8701 taagaatctt acccttatca aattcaaaag cgccaacgtc cttgatgtat aaccgtccac
     8761 ggaacaggat ctttacgtac ttcgccacct gaagtgggtt gtaccgttgg aaaattttca
     8821 ttgttatctc ctgctgagta ttacgcctgt gcggtgccac aatcggccca actattatga
     8881 gacgcaaatt ttaatgctta gtgactatag actatccggg caatgtttcc accgcgtata
     8941 acttttttta cctaaaggtt acaattattc agaattatct ttttaccgaa gcgcggtctt
     9001 cagtataagc attcattttt catatgattt gtgcgcttga ccgcaaactg gcatcacact
     9061 tgcgggaaat tcgataaata gcacatatga ttaaaactca gatccaagtg gtcggatcac
     9121 ctgcatatca taagaaggaa acaccatgac cctacgcaag attctggcac tcacctgcct
     9181 gctgttgccg atgatggctt ccgcacatca gttcgaaacc ggtcagcgag tgccgccgat
     9241 tggcattacc gatcggggcg agttggtgct tgataaagat cagtttagct acaaaacctg
     9301 gaacagcgcg cagttagtgg gaaaagtgcg agtactgcaa catattgctg gtcgcacctc
     9361 tgcaaaagag aaaaacgcga cgctgattga agcgattaaa tcagcaaagt taccgcacga
     9421 tcgttaccag accaccacca ttgttaacac cgacgacgca attccgggtt ccggcatgtt
     9481 tgtgcgcagc agtctggaga gtaataaaaa gctttatccc tggtcgcagt ttattgtcga
     9541 tagcaatggc gtcgcgcgcg gtgcctggca gctggatgaa gaaagttccg ctgtagtggt
     9601 gctggataag gatggtcgcg tgcaatgggc caaagacggg gcgctcacac aggaagaggt
     9661 gcagcaagtg atggacctgc tgcaaaagtt gctcaaataa tggcaataac ataaattctg
     9721 caaaaataat tttcccctat cgttatctat gggatagggg aattaggtct ctgagctctt
     9781 acaccgaact catactggta acgctagttg ctctttttta aacgcctcaa tggttgattt
     9841 atatttctcg caaatctcct cttcggtaat atcactatct acggccagat tcgcacttac
     9901 tatcgcatcc acttgttcgg ctaattcgac ttgttggcaa cctgcattaa ccagaaaatt
     9961 ataaataaga gagtaatctt catcctccaa aacaacagag gaaccattac gaaaacgttc
    10021 atcccctcca tagattttaa gcgcaaagcc caaagattct atgactatgg caaaattatc
    10081 atcaattatt ttttcgtaaa tataccatcc atcgcattct ttaattccac ctcctggtat
    10141 ttggagttca aatttacccc gttttaattt gcttactata tcctccaata aggcataacc
    10201 taaagacacg tcaccaatcg cctggtgaat ataaccttta atccttttcg gtatgtcaga
    10261 ctgttttttc tgcagaatat cccaagcata caatcttctg ttttgttcta acggaaattc
    10321 acggtttttg aaatccatga atgagttata ctcatttatt gttatgggga tttcttcgta
    10381 ttcaaatcca ctgcctcgca atgtcattgt atattcggca ccgttatctt taaggctgaa
    10441 actgataaga aacttatcca tggctttaac agtttcattt tttgatgaaa gcacttgata
    10501 actatcttta tcaatctgag ttgcgcaacg aaaaaactcc cacaaagtta acaggctatc
    10561 ataattcgaa acccaatctt ttttcttaaa tgcatgctga atattattaa aaaaattgtt
    10621 tgaatgtgtt tttgtatgcg atgattttac gaataactcc accgcaccaa taaaattatt
    10681 gtgtctgatc ctgggtaaca tatggctacc ttattttatt tggttatttt atgtcgccat
    10741 taaatatctc attatagaag taaactatca aaaaacgctc attattaaaa tgtaacttat
    10801 tagcaattga atttttgcac tttaaataca atctcttttt atttataact ttttaggttg
    10861 ggagaaatca ggatggcaga atcaggaaat acactgtttc tgccatctga atttagtaaa
    10921 tagacactct gaacccagga ttcaggaacg actcgcgcgg agtgtaatcc agcggtttac
    10981 cctgccagtc gtgaacgtgc gctccggcag ctgcggccac agcatgtcca gcggcggtgt
    11041 cccaaatatt cgttggtccg aagcgcgggt acagctgcgc ctgtccttcc gccaccaggc
    11101 agaatttcag cgaagagccg atggacgtgg tctgatgttc gccaagctgt tgcagatact
    11161 ctttcagctc cgcatctgca tgggaacggc tgataaccac cagcggcggg cgcgcatcgc
    11221 ggacctgaat ctgcttgcgc acaccgcact cttctttcca ggccttgcct tctgccgcgc
    11281 tgtacattac gttcattacc ggcgcataca ccacacctaa aatcggtttg ccatggtcaa
    11341 tgagcgcaat gttaacggtg aattcgccat tgcgtttaat aaactcttta gtaccatcca
    11401 gcgggtctac cagccagtaa cgctgccagt gctgacggac ttcccaaccg ggaggatctt
    11461 cttcagaaag gaccggaata tccggtgtca gcgtacgtaa accgtccatg ataacggtgt
    11521 gtgcggcaat atccgctgcc gttaccggag aattgtccgc tttgctgacg acgtccatcg
    11581 gtttcgtccc gtcgtagacc tgcataatgg catcgcctgc attccgtgca agctggcata
    11641 cttgatctaa catttctcca cctcgtctct gtgagcggtg ttaacttatt gttttactta
    11701 taccctatcg ttaatgaatg cgccaactgt gatagtgtca tcattttcaa agcgtaaaat
    11761 tgtggcattc ttcactgttc tataagtaag acgtttattc tcccttttct ttcgtattcc
    11821 cgatgataaa aggatgtccc tgatgattaa gtttagcgca acgctcctgg ccacgctgat
    11881 tgccgccagt gtgaatgcag cgacggtcga tctgcgtatc atggaaacca ctgatctgca
    11941 tagcaacatg atggatttcg attattacaa agacaccgcc acggaaaaat tcggactggt
    12001 acgtacggca agcctgatta acgatgcccg caatgaagtg aaaaacagcg tactggtcga
    12061 taacggcgat ttgattcagg ggagtccgct ggccgattac atgtcggcga aaggattaaa
    12121 agcaggtgat gttcatccgg tttataaggc gctgaatacg ctggattata cggtcggtac
    12181 actcggcaat catgaattta actacggtct ggattacctg aaaaatgcgc tggcgggagc
    12241 gaaattccct tatgtaaatg ccaacgtcat tgacgccaga accaaacagc caatgtttac
    12301 accgtattta attaaagata ccgaagtggt cgataaagac ggaaaaaaac agacgctgaa
    12361 gattggctat attggcgtcg tgccgccgca aatcatgggc tgggataaag ctaatttatc
    12421 cggaaaagtg acggtgaatg atattaccga aaccgtgcgc aaatacgtgc ctgaaatgcg
    12481 cgagaaaggt gccgatgtcg ttgtcgttct ggcgcattcc gggctgtctg ccgatccgta
    12541 taaagtaatg gcggaaaact cagtttatta cctcagtgaa attccgggcg ttaacgccat
    12601 tatgtttggc catgctcacg ccgttttccc gggtaaagat tttgctgata tcgaagaggc
    12661 tgatatcacc aaaggcacgc tgaatggtgt tccggcggta atgccgggca tgtggggcga
    12721 tcatcttggg gtggtcgact tacaactcag taatgacagc ggtaaatggc aggtgacgca
    12781 ggcgaaagcg gaagcacggc cgatttacga catcgccaat aaaaaatccc tcgcggcgga
    12841 agacagcaag ctggtagaaa cactcaaagc cgatcacgat gccacacgcc agttcgtcag
    12901 caagccaatc ggtaaatctg ccgacaatat gtatagctat ctggcgctgg tgcaggacga
    12961 tccgaccgtg caagtcgtga acaacgcgca aaaagcgtat gtcgaacatt acattcaggg
    13021 cgatccggat ctggcaaaac tgccggtgct ttcagctgcc gcaccgttta aagttggtgg
    13081 tcgcaaaaat gacccggcaa gctatgtgga ggtggaaaaa ggccagttga ccttccgtaa
    13141 tgccgccgat ctttatctct accccaatac gctgattgta gtgaaagcca gcggtaaaga
    13201 ggtgaaagag tggctggagt gctccgccgg acagtttaac cagattgatc ctaacagcac
    13261 gaaaccacag tcactcatca actgggatgg tttccgcact tataactttg atgttattga
    13321 tggtgtgaat tatcagattg atgttaccca acccgcccgc tatgacggcg agtgccagat
    13381 gattaatgcc aatgcagagc ggattaagaa cctgaccttt aatggcaagc cgattgatcc
    13441 gaacgctatg ttcctcgttg ccaccaataa ctatcgcgct tacggcggca aatttgccgg
    13501 tacgggcgac agacatatcg cttttgcttc accggatgag aaccgctcgg tgctgacagc
    13561 gtggattgct gatgagtcga aacgtgcggg agaaattcac ccggcggcag ataacaactg
    13621 gcgtttagca ccgatagctg gcgataagaa actggatatc cgtttcgaaa cttccccgtc
    13681 agataaagcc gcagcgttta ttaaagagaa agggcaatat ccgatgaata aagtcgcgac
    13741 cgatgatatc gggtttgcga tttatcaggt ggatttgagt aagtaaaaca cttctttttc
    13801 ggtctataaa ttatcaaccg catccggcat ttattggcgg atgcgatgct ggcgcatctt
    13861 atccgcccta ccagccatgc accgtagacc agataagctc agcgcatccg gcagttatgc
    13921 cgcatgttca tcccgcaccg ccaacacctc gggcaaattc aactcaatcc agtccgccag
    13981 tgcagcaacc ttttcgctca cctgctcgcc cagcggcgtg aggctatatt ccacatgcgg
    14041 tggcaccacc ggatacgcga tacggttaag aaaaccatcc tgttctaacg cctgtaacga
    14101 ctgcgcaagc atcttttcgc tccccccgcc gattttgcgc cgcaggtcgc taaagcgatg
    14161 agtaccttcg cgtagcgcca ccagaatcaa caccccccaa cggctggtga cgtgtttcaa
    14221 cacctcgcgc gacgggcact gttccgcaaa gagattaccc tctttcagtt gttgcgacag
    14281 gctaacctga ctcatttcat acttaccttt ttgtacgtac ttactaaaag taagtttagg
    14341 tgttagcgta tttaaacaca agacaaaacg atggagactt cccatgatcg ctattactgg
    14401 tgccactggc caacttggtc actatattat tgaatccttg atgaaaacgg ttcctgccag
    14461 ccaaatagtg gctatcgttc gtaatccggc aaaagcccag gctctggcag cacaaggcat
    14521 taccgtgcgt caggctgact acggcgatga agccgcactg acatctgcac ttcagggagt
    14581 ggaaaaacta ctgctgatct cttccagcga agtgggtcaa cgtgccccgc agcatcgtaa
    14641 tgttattaat gccgcaaagg cggctggcgt gaaatttatc gcttatacca gcctgctaca
    14701 tgcagatacc tccccgctcg gcctcgccga tgagcacatt gagacggaga aaatgttggc
    14761 tgattctggc atcgtttaca ccctgctgcg caacggctgg tacagcgaaa actacctcgc
    14821 cagcgccccg gcagcactgg aacacggcgt atttatcggt gcggcgggcg atggcaaaat
    14881 cgcctcagca acgcgggcag attatgcggc agctgcggca cgcgtgatta gcgaagccgg
    14941 tcacgaaggc aaggtttacg aactggcggg cgatagtgcc tggacgttga cacagttagc
    15001 ggcagagctg accaaacaga gcggcaaaca ggttacctat caaaatctga gcgaagccga
    15061 tttcgccgcg gcactgaaaa gcgtcggact gcccgacgga ctggcggata tgctggcgga
    15121 ttctgacgtt ggcgcatcga aaggtggtct gtttgataac agcaaaacgc ttagcaaatt
    15181 gattggccgc ccaacgacaa cgttagccga aagcgtaagc catcttttta atgttaataa
    15241 ctagttaatt aaagtggcat cctcccgcat cctctctaat aatgacggga tgccgggagc
    15301 aatcatgtct gcttcctgaa ctttcttctg acagaccaat ggatgccagt aatgattagc
    15361 ggcgtgctgt acgccctgtt agcagggttg atgtgggggc ttatttttgt cgggccgttg
    15421 atcgtaccgg aatacccggc gatgttgcag tcgatggggc gttatctggc gttagggtta
    15481 attgcgctgc ccattgcctg gctgggacgc gtgcgtctgc gtcagttggc gcgtcgcgac
    15541 tggcttaccg ccttgatgct cactatgatg ggcaacctca tctattactt ctgccttgcc
    15601 agtgccattc aacgtactgg tgcgcctgtt tccacgatga ttatcggcac cctgccggtg
    15661 gtgattcccg tttttgccaa tctgctttat agccagcgcg acggcaaact cgcgtgggga
    15721 aaactcgccc cggcactggt ttgtattggc atcggcctgg cgtgtgtgaa tattgctgag
    15781 ttaaaccacg gactccccga ttttgactgg gcacgttata cctcaggcat cgtgctagcg
    15841 ttagtttccg tggtctgctg ggcatggtat gccctgcgca acgcccgctg gctgcgggag
    15901 aatcccgaca aacatccgat gatgtgggcg acggcgcagg cgctggtcac actgccggtt
    15961 tctctcatcg gctatctcgt cgcctgttac tggctgaata cgcaaacgcc ggacttctcc
    16021 ctaccttttg gcccccgtcc gctggtgttt attagtctga tggttgcgat agccgtgctt
    16081 tgctcatggg ttggcgcact ctgctggaac gtcgccagcc agcgattacc gacagtgatt
    16141 ctcgggccgc tgatcgtttt cgaaacactg gcaggtttgc tgtacacctt tttgatacgc
    16201 cagcaaatgc cgccgctgat gacgctgagc ggtatcgcgc tgttagtggt tggcgtggtc
    16261 attgtagtca gagcaaaacc ggaaaagcct ttaactgaat ctgtctcaga aagttgacat
    16321 actggctgtg agttaaataa gcctctgcta cgtaagggtt atagcttttg ccttaaagat
    16381 gtatttaaaa tacatcttat cttattaaga atgaggtatc agctatggct tatcgcgacc
    16441 aacctttagg tgaactggcg ctctctattc ctcgcgcttc agctctgttt cgtaaatata
    16501 atatggatta ctgctgtggc ggtaagcaga cgctggcgcg cgcggcggca cgtaaagaac
    16561 tggatgttga ggtcattgaa gctgaactgg caaagcttgc tgaacaaccg attgagaaag
    16621 actggcgtag cgccccgctg gcagaaatca ttgaccatat catcgtgcgc taccacgatc
    16681 gtcaccgcga gcaactgccg gagctgatcc tgcaagcgac taaagtcgag cgcgttcacg
    16741 ccgacaaacc gagcgtgcca aaagggctga caaaatacct gaccatgctg catgaagagc
    16801 tttccagcca catgatgaaa gaagagcaga tcctcttccc gatgatcaaa caaggcatgg
    16861 gcagccaggc aatggggcca atcagcgtaa tggaaagcga gcacgatgaa gcgggcgaac
    16921 tgctggaagt gatcaaacac actaccaata acgtcacacc gccgccagaa gcctgcacca
    16981 cctggaaagc gatgtataac ggcattaatg aactgattga tgacctgatg gatcacatca
    17041 gtctggaaaa caatgtactg ttcccacgcg cgctggcggg tgagtgatct aaaagctgga
    17101 tggcattgcg ccatccagca tgataatgcg gttatttccg cagttcagca gcccgcttct
    17161 taccaataaa caaccagccc agccccagtg cgataaacca cagcggggta accagcagcg
    17221 cctggcgagt gtcatcttcc agtgtcagca acacgaccac gaacacaaag aacgccatac
    17281 atacccagca catcagcttg ccgagcggca tcttgtagat cgacttctca tgcagatgag
    17341 gacgctgttt gcggtacacc aggtacgagc aaaggataat cgtccagacg aacataaaca
    17401 gaatcgcgga aacggttgta atcatcgtga acgcaccaat cacgctagga ttcacataca
    17461 gcatcaccac gccaccgagc aggcagatgc aggagaaggt cagccctttc gctggtactg
    17521 cgcgcttaga aagtttagcg aacgctttcg gtgccacacc ttcctgcgcc agaccaaaca
    17581 gcatacggct ggtagagaag acgccgctgt tagcggaaga cgccgcagag gtcagcacca
    17641 caaagttgat cacgctggca gccgcaggca aacctaccaa cacaaacagc tcaacgaacg
    17701 ggcttttctc cgggactacc gaactccacg gtgtcacgga cataatcaca atcagcgcga
    17761 agacgtagaa cataatgata cggatcggaa tggagttaat cgcgcgtggc agtgatttct
    17821 ccggatcttt ggtttccgca gctgttgtac ctaccagctc aatccccacg aaagcgaaaa
    17881 ccgctatctg gaatccggca aagaagccac ttaaaccttt cgggaaccag ccgccgtcat
    17941 tccacaaatg cgcgaatgac gcttccacac cagtcggtga ctgaaagtgc atcgccacca
    18001 tgaccaggcc gacgacaatc agcgacacga tggcgacgat tttgatcatc gcaaaccaga
    18061 actccatctc accgaacatt ttcacggtgg caaggttgag cgtcagcagc agcactatca
    18121 ccgccagcga ggcgacccag tcggagagat cggggaacca gaactgagca taagccgtga
    18181 tcgccaccac gtctgccata ccggttacaa cccagcagaa ccagtaagtc cagccagtga
    18241 aatatcctgt ccacggcccg agtaaatcgg aagcgaagtc actaaaagat ttgtattcca
    18301 gattcgaaag cagcaattcc cccattgccc gcatcacgaa aaagagcata aaaccaatga
    18361 tcatataaac gaaaatgatc gacggcccgg caaggctaat cgttttgcca gaccccagaa
    18421 acaaccccgt accaatggca ccgccaatgg caataagctg aatatgtcgg tttgtgagat
    18481 tgcgccgtag cgactgttca gccggagcct gatcatcggc aacgactttt acctgatcta
    18541 ccatgttttt ttcttcctgt acctgtctgt gttgttcagg ctctacggcc tttagtcagt
    18601 ctatgatacg acgaatctgt atcgggactc atcgatatta ggtaacaata cgcgggatga
    18661 atactaagat ttagatctaa tgttaatttt atgtttaaag tgagtgtcat atcacggtga
    18721 taacgcgaaa catctcacaa aaatacacgc ttacggggtc tttgcaacac aaaatagcgc
    18781 cgtttggcaa actgaagggt ttattgctga atgcctgctc ccctctggtt cgaggggaac
    18841 agaatgcgct gcttagagga tttccagcag ttcgacttca aacaccaggg tgctgaacgg
    18901 agggatggat gcacctgcgc cgcgctcgcc atatgccagt tcctgcggga tagtcagttc
    18961 ccatttagaa cctaccggca tcagagtcag tgcttcaatc cagccaggga tcacgccgtt
    19021 aaccgggaat tcagcgggtt caccacgagc aacggagctg tcaaacacgg tgccgtcgat
    19081 cagtttaccg gtgtaatgaa cacgaacgcg gtcggtacgt gccggaattg cgccttcacc
    19141 ctggttgatc acgcggaatt gcaggccaga ttcggtgcta ttcacacctt cttttttggc
    19201 gttttcttcc aggtatttca caccttcagc agccatcgcc tggaaacgct gacgacgaac
    19261 ggcatcggcg cgttcgtgga tttcacgcag cgcgcgatgc accacatcaa ccggaacagc
    19321 cggatgtttg ccttccagcg catcggcaat acctgcaacc agtgcttctg gcagcagccc
    19381 ttccaggcca gattcactca gttgttgccc tacctgcaaa ccaatgccgt agcttgcttg
    19441 cgcttcgatg gtgtcaaaag ttggggtggt catcgtattt cctttcttag ggtataaaag
    19501 tagcgggcag catatcagcc acacttcttt gggtaaaact ttgtcacagg aatgatgaca
    19561 aatcccgggg aaaaagaaac aatagcaata tcccgt
//
LOCUS       DAJVUJ010000072        19465 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.072, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000072 DAJVUJ010000000
VERSION     DAJVUJ010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19465
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.072"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            6..81
                     /locus_tag="OQQ48_002273"
     tRNA            6..81
                     /locus_tag="OQQ48_002273"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Thr,seq:ggt)
     gene            121..236
                     /gene="rrf"
                     /locus_tag="OQQ48_002274"
     rRNA            121..236
                     /gene="rrf"
                     /locus_tag="OQQ48_002274"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(468..1226)
                     /locus_tag="OQQ48_002275"
     CDS             complement(468..1226)
                     /locus_tag="OQQ48_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCS1953030.1"
                     /translation="MSQILLQPANAMITLENVHKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFTHPKSERTRAFLSQVIH"
     gene            complement(1234..2337)
                     /locus_tag="OQQ48_002276"
     CDS             complement(1234..2337)
                     /locus_tag="OQQ48_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCS1953031.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     VLLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2347..3517)
                     /locus_tag="OQQ48_002277"
                     /pseudo
     CDS             complement(2347..3517)
                     /locus_tag="OQQ48_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
     gene            complement(3585..4658)
                     /locus_tag="OQQ48_002278"
     CDS             complement(3585..4658)
                     /locus_tag="OQQ48_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HCS1953032.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LLGFSYADADGKFSGIDVDVDVDVDVDVDVDVDVDVCRSVAAAVFGDDTKVKYTPLTA
                     KERFTALQSGEVDLLSRNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKEL
                     DGATVCIQAGTDTELNVADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQL
                     YALRIKLSNPAEWIVLPEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQ
                     NVDEKAANPATPDMAHLLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESP
                     LKIKRGQNNLWNNGGIQYAPPVR"
     gene            complement(5089..5310)
                     /locus_tag="OQQ48_002279"
     CDS             complement(5089..5310)
                     /locus_tag="OQQ48_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1953033.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGCLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
     gene            complement(5563..8667)
                     /gene="acrF"
                     /locus_tag="OQQ48_002280"
     CDS             complement(5563..8667)
                     /gene="acrF"
                     /locus_tag="OQQ48_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
                     /protein_id="HCS1953034.1"
                     /translation="MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVS
                     VSANYPGADAQTVQDTVTQVIEQNMNGIDNLMYMSSTNDSAGSVTITLTFQSGTDPDI
                     AQVQVQNKLQLATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVAS
                     NVKDTLSRLNGVGDVQLFGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQ
                     LGGTPALPGQQLNASIIAQTRLKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYN
                     VIARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQ
                     LSIHEVVKTLFEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLS
                     AVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPVSAEHHENK
                     GGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEE
                     DQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAG
                     MAFVSLKPWEERNGDENSAEAVIHRAKMELGKIRDGFVIPFNMPAIVELGTATGFDFE
                     LIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALSV
                     SLSDINQTISTALGGTYVNDFIDRGRVKKVYVQADAKFRMLPEDVDKLYVRSANGEMV
                     PFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMALMENLASKLPAGIGY
                     DWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLA
                     ATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLR
                     PILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFLPVFFVVIRR
                     CFKG"
     gene            complement(8679..9836)
                     /gene="acrE"
                     /locus_tag="OQQ48_002281"
     CDS             complement(8679..9836)
                     /gene="acrE"
                     /locus_tag="OQQ48_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HCS1953035.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYNSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     GTVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMYVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
     gene            10235..10897
                     /gene="acrS"
                     /locus_tag="OQQ48_002282"
     CDS             10235..10897
                     /gene="acrS"
                     /locus_tag="OQQ48_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HCS1953036.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIINGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
     gene            complement(10900..11079)
                     /locus_tag="OQQ48_002283"
     CDS             complement(10900..11079)
                     /locus_tag="OQQ48_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HCS1953037.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(11163..12047)
                     /gene="yhdJ"
                     /locus_tag="OQQ48_002284"
     CDS             complement(11163..12047)
                     /gene="yhdJ"
                     /locus_tag="OQQ48_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCS1953038.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKIPAESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYDPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDII
                     LDPFAGSFTTGAVAVSSGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(12133..12429)
                     /gene="fis"
                     /locus_tag="OQQ48_002285"
     CDS             complement(12133..12429)
                     /gene="fis"
                     /locus_tag="OQQ48_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HCS1953039.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(12455..13420)
                     /gene="dusB"
                     /locus_tag="OQQ48_002286"
     CDS             complement(12455..13420)
                     /gene="dusB"
                     /locus_tag="OQQ48_002286"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HCS1953040.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(13749..14630)
                     /gene="prmA"
                     /locus_tag="OQQ48_002287"
     CDS             complement(13749..14630)
                     /gene="prmA"
                     /locus_tag="OQQ48_002287"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCS1953041.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(14642..16093)
                     /gene="panF"
                     /locus_tag="OQQ48_002288"
     CDS             complement(14642..16093)
                     /gene="panF"
                     /locus_tag="OQQ48_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HCS1953042.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVETLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(16083..16325)
                     /locus_tag="OQQ48_002289"
     CDS             complement(16083..16325)
                     /locus_tag="OQQ48_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HCS1953043.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLAAAYLPDVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(16418..17353)
                     /locus_tag="OQQ48_002290"
     CDS             complement(16418..17353)
                     /locus_tag="OQQ48_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001446002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCS1953044.1"
                     /translation="MGELLAEFLSRNPHQKFTQPGEFIGPFPSGAPAIFAAQVAKLSH
                     RAIFFGCVGNDDFGRLIIERLRHEGVITDGIHVMNNAVTGTAFVSYQNPQQRDFVFNI
                     PNSACGLFTAEHIDKDLLKQCNHLHIVGSSLFSFRMIDVMRKAITTIKSAGGTVSFDP
                     NIRKEMLSIPEMAQALDYLIEYTDIFIPSESELPFFARHKNLSEEQIVSDLLHGGVKH
                     VAIKRAQRGASYYKLKNGTLHAQHVTGHDIEIIDPTGAGDCFGATFITLFLSGFPAHK
                     ALQYANASGALAVMRQGPMEGISSLADIEDFLQQH"
     gene            complement(17385..18215)
                     /locus_tag="OQQ48_002291"
     CDS             complement(17385..18215)
                     /locus_tag="OQQ48_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCS1953045.1"
                     /translation="MKSVNVPRKSSNLLKRRLKIAEIVATQGEIKVDDLSAQLGVSGV
                     TIRGDLSYLEQQGYLKRSFGGAIATPLSSEPEAIQPIAPLSLAQQMEIARHCARLIRD
                     RDTLFLGHGTICRKIIPLLSEVKKLRLITNDPEHALLANQFIDGEIILAGSEMLRPDT
                     ALAGKPLEQALQHFTITHSILEINHLDADGTLNINVPRLAAAWQLCFDHAQNKTVIVA
                     ADPAPSTAAIGHLHQAENVIVSRSINEQYQQQLQSADFVILYTSNECFTWSNRERLQE
                     "
     gene            18558..19412
                     /locus_tag="OQQ48_002292"
     CDS             18558..19412
                     /locus_tag="OQQ48_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose bisphosphate family class II aldolase"
                     /protein_id="HCS1953046.1"
                     /translation="MYIISSKNMLKKAQSEGYAVPAFNIHNLETLQVVVESAAQLRSP
                     VMLAGTPGTYRYGGVGSLISLVQSLAREYNLPLVLHLDHHEESDDIFNKVRAGIRSVM
                     IDGSHLPFEDNIARVQEVTTFCHRFDVSVEAELGRLGGQEDDLLVDEKDSAYTSPAAA
                     AEFVQRTQIDSLAVAIGTAHGLYAHEPRLDFVRLEQIRQRVEIPLVLHGASGLSEQDV
                     QHCIRRGICKVNVATELKIAFADALKGYFYNNPAANDPRHYMQPAKAAMKEVVTRIIG
                     VCGSEGKI"
BASE COUNT         5166 a         4884 c         4648 g         4767 t
ORIGIN      
        1 agcgtgctga tatggctcag ttggtagagc gcacccttgg taagggtgag gtccccagtt
       61 cgactctggg tatcagcacc actttttagg ttaaagttcg gcaaattaga aaagaatttg
      121 tctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      181 cgtagcgccg atggtagtgt ggggtcttcc catgcgagag tagggaactg ccagacatca
      241 aataaaacaa aaggcccagt cttccgactg agccttttgt tttatctgtt gtttgtcggt
      301 gaacactctc tcgagtagga caaatccgcc gggagcggat ttgaacgttg cgaagcaacg
      361 gcccggaggg tggcgggcag gacgcccgcc ataaactgcc agacatcaaa tcaagcaaaa
      421 aggccatccg aaaggatggc ctttttgctt ttcgaactaa cattcaatta atggattacc
      481 tgcgataaaa atgccctcgt acgctctgat ttaggatgcg taaaaaattc atcaggtgca
      541 gcttgttcca ctatctcccc acgatccata aaaattaccc ggtcagcgac ggtacgtgca
      601 aaccccatct catgtgttac acacaacatt gtcatacccg actgcgccag cccaatcatc
      661 gtatccagca cctctttcac catctccgga tcgagcgccg acgttggttc atcaaacaac
      721 ataattttcg gcttcataca cagcgaacgc gcaatggcaa cgcgttgctg ctgaccacct
      781 gaaatctgtc cgggaaactt atgcgcatgt tcggcaattc tcacccgctc aaggtaatgc
      841 atcgccagag cttcagcctc tttcttaggc atcttgcgta cccaaatcgg tgccagggta
      901 cagttctgta aaacggtcag atgagggaag agattgaaat gctgaaatac cattcccact
      961 tcttgcctga cgcgctcaat attgcggata tcttcattaa gttcgatgcc atctacgaca
     1021 atccgtccct gttgatgttc ttccagatga ttaatacaac gaatggttgt cgatttaccg
     1081 gaaccagaag ggccacacag aacgatccgt tctcccggtt gcacggttaa atttatattt
     1141 ttcaaaacat ggaattgtcc ataccattta tggacgtttt ccagcgtaat catcgcgtta
     1201 gcaggttgca gtaaaatttg gctcatagtg tcctcaatgc ggtgtacgcc cggtgttaaa
     1261 acgtttttcc agatgttggc tataacgtga catgctgaaa caaaagatcc agtagatcag
     1321 cgcggcgaaa acataccctt ccgtcgacat acccaaccag gcgggatcaa cggttgcctg
     1381 ctgaacgcta ctgaaaagat cgaacaagcc gatgatgatc accaggctgg tatctttgaa
     1441 gagtgcgatg atggtattta ccagcccagg aattaccagc ttcaacgcct gtggcagaat
     1501 aaccagcccc tgagttttcc agtaccccaa cgccagcgac tctgccgctt catattgccc
     1561 tttaggtagg gcctgtaatc cgcctcgcac gacttccgca acatatgctg actgaaacag
     1621 gatcacgcca accagcgcgc ggatcagttt gtcgatactg gtgccttctg ccataaacaa
     1681 cggcagcatg accgaagaca taaacaatac ggtaatcagc ggtacgccgc gccagaactc
     1741 gataaaaatg accgataaga tacgcacaat cggcatatgg gagcggcgac ctaacgccag
     1801 taagatcccc cacggtagcg cccccgcaat cccaacggat gcaataatta aagttagcgt
     1861 cagcccgccc cattgccggg tttcaacccg ctcaagacca aaaaaaccgc catacatcag
     1921 ccaccagaca atcagtgggt aaatcaccgc ccagaccgca atatagcgac cgcgatgcgg
     1981 gagtattttc cagaacattg gtgcgattga cacaaggcca atcagtaata ccaggttaat
     2041 tcgccagcgt tggtcgtgtg ggtaaagtcc atacataaac tgaccaaatc gttcgtggat
     2101 gaagacccaa caggcaccgg cttttgtgca gtcggcacgc gttgagccaa cccagttagc
     2161 ctgcaaaaat gcccagttca gcaacggtgg gatcagttcc cacatcaacc atatgcagcc
     2221 aatagtcagc aggctattgc tccagctgga gaacagattt tttcgcaccc acaccatcgc
     2281 gcggcttgag ttatggctcg ctgggcgcga gggctgagac agcaatactt ttgtcatcat
     2341 gactccttag cgttcaacga tcgcgatgcg gcggttatag atattcatca gcagcgagat
     2401 agtcaggctg ataatcagat agaccgacat ggtcatggct atcgtctcga tggcttgccc
     2461 cgtctggttc agcacggtgc cagcaaacag cgaaaccata tcgggatagc caatagcggc
     2521 ggcaagagag gagtttttga cgatgttgag atactggctg gttaacggcg gaataatcac
     2581 tcgcagtgcc tggggaataa tgacctggcg tagcgtaacc ccagtgaccg agccgcttca
     2641 tgttgaccat aaggcactgc ctggatccca gcgcggataa tctcggcgat aaatgcagag
     2701 gtataaaccg aaagtgccag cgttaaggct gccagttcag gaattaaaac cattccgccg
     2761 cggaagttaa agcctcgtag ggctgggaca tcccagtgta gtgctgcgcc aaaaagccat
     2821 tgcgccagta aaggcaaacc gatgatcaac actgcggcga acggccaggt gcggcgcagt
     2881 tgtccggtct ttatctggta tgttttatta aagcggaata gcccaacaga aaggactata
     2941 gccataacaa cagccaggat aaacgcaata aatccgtctc ccagctgcgg tgacggaata
     3001 taaagcccac gattgctcaa aaaggccaga tcaaacgcgc tcacagcctg gcgcgtggtc
     3061 cgggcaaatt gcgcaacacg gcaaagtacc agaagaagat ttgcagcagc gggggaatat
     3121 tacggaagat ctcaatataa attgtggata gctttcgtag cagtcagtta tccgaaagtc
     3181 tcgtcagacc gataaagaag cctaaaacag aagcgaacac aatacacaat gcagaaacca
     3241 gtagcgtatt gagtaagccg acaataaaaa cgcgtccgta ggtgtcgccc tgctggtaat
     3301 cgatcaaatg ctggacaata ccgaagccag cgccgcgatc cagaaaggca aaacctgaag
     3361 taatgccacg attactgaga ttcgttacag tgttgtgaaa taaccaacca acaattccga
     3421 caacagcaac aacggcaagg atctggaata accaggcacg aaccgtaggg ttggcaaagg
     3481 agagtgagcc tttaacggtt gagcggcgat gagacataga aacctcggaa agcataacgc
     3541 tgaagagagc gccgccgtag cgacgctcgt tacctcacac cgacttaacg cacgggcggc
     3601 gcgtactgaa taccgccgtt attccagaga ttattttgcc cacgtttaat tttcagcggg
     3661 ctttctgaac ctacgttacg ctcgaaaatt tccgagtagt tacccacctg tttgatgatg
     3721 ttataggccc atttattatc caacttcaga tccttgccgt aatcgccttc tttacccagc
     3781 agatgtgcca tatcaggcgt tgctggatta gccgcttttt catcgacgtt ctgggaattg
     3841 atgcccatct cttcagcatt cagcatggcg aaaagcgtcc agcgtacaat cgagaaccat
     3901 tcatcatcgc cacgacgaac taccggccca agcggttctt tagagataac ctccggtaag
     3961 acaatccatt cagctgggtt gcttaatttg atgcgcaggg catacagttg cgactgatcc
     4021 gaggccagcg tatcgcagcg accagattcc agtgccttcg ctgattcgtc agagcgatcg
     4081 aaagtcaccg gtgtgtactt catattgttt gccttgaaat agtcggcaac gttgagttcg
     4141 gtatcagtac ccgcctgaat acagacggta gcgccatcca gttctttcgc gctttttagc
     4201 cccgctttat cgtgcgtcag gaagccaatg ccgtcgtaat aagtgacgcc agtaaatgcc
     4261 attcccatcc cggcgtcgcg agatgaagtc caggtcgtat tacgggagag caaatccact
     4321 tcccctgact gtaaagccgt gaagcgttct tttgctgtga gtggggtata tttcactttc
     4381 gtgtcgtcac caaatacagc agcggcaaca ctacgacaaa catcaacatc aacatcaaca
     4441 tcaacatcaa catcaacatc aacatcaaca tcaacatcaa tacctgaaaa cttaccgtct
     4501 gcatcggcat aagagaaccc aagtaatcca tcactgatcc cgcattgcac aaaacctttt
     4561 ttctgaacgg catcaagcgt cgcgccagca tgcgcctgat ttgcaacggc aagcagcacg
     4621 ctggcggcag ccagtgtggc tatcatcatc tttttcataa tgcttcctgt gaggcgaaaa
     4681 ttatcgttat aggacgtttg ggaaacgtct tttacctgtc tgtggccatc ctcttataag
     4741 caaaagtagt gccagctttt gcgcacgatt tcgcgtcgca ataagctgag tgtagacagg
     4801 aaataataaa aacagcgccc cgcaatggtg cgaaaatgaa acatgcgcca cattttggcg
     4861 caaaatattt acgcgtgttt tactaagcag aataatgatt gttagcgtga gtatatttct
     4921 ggtgggaagt gtgaaagtta aattgatgcg ttatttcgaa agcagcgaaa taacgcgata
     4981 taacatgcta aatcagcaga gaattagcca gcagaatata gcaatatttt atcttatgta
     5041 aagatactgt atcgttaaac agagcgcgaa actccgcgcc ctgttcagtt atttcgacag
     5101 agcgatgatc cccatcgtca ggccagcaac agccgctgcc ccgccggcaa tagccccagc
     5161 cgtttcccag ttatcctgag tcgtgccttt catacaggta ttggtgtgaa ccagacaacc
     5221 ctgatcgtca taaaccggaa cgcagggcga atcgtgagca cagcccgcca gcaatgcggt
     5281 gagcaatgcc acaggaatta atcttttcat ggtgttacct cagtaataaa cgctaacgct
     5341 taataagtta actctcttat taacgagctg aatgccattt tatcaccgga ggttataaat
     5401 attgcggaat gaataatgac aaattatgtg aattgtaaaa atagtgaagg gagcgggaac
     5461 tatagaaagt ttttaaagag tgagtggaag aatatcttat taatttttat gggtttcact
     5521 gaaaataata aaggcacccg aaagtgcctt tatgtttctg atttatcctt taaagcaacg
     5581 gcggatcacc acaaagaaca ccggtaggaa gaagattgcc agcaacgttg cagagaccat
     5641 tccgcccatt accccgatac ccactgcgtt ctgcgcgcca ctgcctgcac cgttactgat
     5701 agctagcggt aatacgccga gaataaaggc gagagaggtc atcaggatag gacgcagacg
     5761 catacgtact gccattagtg tcgcttcaac aacaccttta ccctctttct ccatgagatc
     5821 tttagcgaac tcaacaatca aaatagcgtt tttggccgac aagccaattg tcgttagcaa
     5881 gcccaccata aagtagacgt catttttttg attaaagagt gtcgccgcca gcagcacgcc
     5941 gacaatccct aacggcacga ccaacataac cgagacagga attgaccagc tttcatagag
     6001 tgcagcaagg cacaggaaaa caaccacaaa ggaaattgct actagagcgg gagcctggtt
     6061 tcccgataag cgttcctgat acgacatacc cgtccagtca taaccaatgc ccgcaggtaa
     6121 ttttgacgca aggttttcca tcaacgccat ggcatcgccg gaactggttc ctggcgcggc
     6181 ttccccctga atctccattg acggcagacc gttgtagcgt tccagtcgcg gagagccata
     6241 cacccaatgt gaagtggtaa aggccgagaa tggcaccatt tcgccgttgg cgctgcggac
     6301 ataaagttta tcgacatctt ctggcagcat acggaatttg gcatccgcct gaacatacac
     6361 ctttttcacg cgaccacggt cgatgaagtc gttaacgtaa gtcccaccca acgccgttga
     6421 aatggtctga ttgatgtcag aaagtgaaac acttaatgcc tgcgcctttt cctggtcaac
     6481 ttccagttta aactgcgcgg tgtcttccag gccattagga cgcacgctga ctaagctggc
     6541 aggatgttgc gccgccatac caagcaactg gttacgggcc tgggttaggg catcgtgacc
     6601 cagcccagcc tgatcaatta actcaaagtc gaaacccgtt gccgtgccca gttcaacaat
     6661 ggctggcata ttgaatggaa tgacaaaacc gtcgcggatc ttgcccaatt ccattttggc
     6721 acgatggatt accgcttccg cactgttttc atcaccatta cgctcttccc acggtttcag
     6781 actgacgaag gccataccgg cgttttgtgc ctggccgctg aagctaaagc cgttaaccgt
     6841 aaagacactt tcaacgttcg ctttctcgtt cttcagatag taatccgtca cttgatccaa
     6901 cactttttgc gtccgctctt gcgtcgcgcc agcgggtaac tgaatcatgg tcagaaagac
     6961 accctgatcc tcttcaggta agaaggaaga cggaagacgt aaaaacaaca ccaccattcc
     7021 tgcaacaatc agcgcataga tcagtaaata tcgtcctgtg gaaccgagga ttttgccgac
     7081 gctgttggtg tagtggttaa cgctatgatc gaaggtggta ttaaaccagc cgaagaaacc
     7141 gcccttattt tcgtgatgct cagcagagac gggtttaagc agcgttgcac ataacgcagg
     7201 ggtaagaatc aatgccacca gaacagaaag tgccattgcc gaaacgatgg tgatagagaa
     7261 ctggcgataa attgccccag tagaaccgcc gaagaatgcc atcggaataa ataccgctga
     7321 cagcaccatc gcgataccca ccagtgcgcc ctgaatttgc gacatcgatt tttccgtcgc
     7381 ttcttttggc gggagcttat cctccatcat cacgcgctcg acgttctcca ccaccactat
     7441 cgcatcatcg acgagcagcc cgatggcaag caccatcccg aacatcgtta gtgtgttgat
     7501 ggagtaacca aaagcggcga ggatggcaaa cgtccctaac aacaccacgg gtaccgcaat
     7561 ggtggggatc agcgttgctc gcatattctg caagaacaga tacatcacca ggaacaccag
     7621 cataatggct tcgaacagcg tttttaccac ttcgtgaata gaaagctgga cgaatggcgt
     7681 ggtgtcataa gggtagagaa ccttcattcc ctgcgggaag aatggctgta attccgccag
     7741 ttttgcctta atggctttcg cggtatcgag agcattcgcg ccggttgcca gcttaatccc
     7801 caggcccgcc gccggttttc cgttgatacg agcgataacg ttatagtttt caccgccaag
     7861 ttcaacccgt gcgacatctt tcaggcgtac caccgagccg tcactgttta cgcgcagggt
     7921 cactttgccg aattcttccg gattttttaa ccgcgtttga gcaataatcg aggcattcaa
     7981 ctgttgccct ggtaacgctg gcgttccgcc caactgtccg gcagcgatct gatcgttctg
     8041 taccttcaac tggttaatca catcaaccgg tgtcagttta tatttgttta gcagatcggc
     8101 gtccagccag atacgcatcg catactgtgc gccgaaaagc tgtacgtcac cgacgccatt
     8161 cagacggcta agcgtatctt taacgttaga ggccacatag tccgagatat cgtcctgtgt
     8221 ggtgcctggg ttatcagaga caaaacccgc caccatcaaa tagctgctac tggacttttc
     8281 aacactgatc ccctgctgct gaacctcctg cggcagcaac ggcgtggcga gctggagttt
     8341 gttctgcacc tgcacttgcg cgatatcagg atcggtcccg gactggaagg taagggtaat
     8401 tgtcacgcta ccggcggaat cgttggtgga ggacatatac atcaggttat cgataccatt
     8461 catattctgt tcgataacct gcgtcaccgt atcctgcacg gtctgcgcat cagcacctgg
     8521 atagtttgcc gaaacagaaa ccgcaggcgg tgcaatggtt ggatactgag cgacgggcaa
     8581 ttgtaggatc gccagtgcgc ccgccatcat cagaataatg gccagcaccc atgcaaatat
     8641 cggtcgtcga ataaaaaagt ttgccatgtc agattacctt acttcgatgc agtatctgcg
     8701 ggggtatcgg tagtggcttt aacctgctct cccggacgcg ctttttgcag gccgctgaca
     8761 atgacttgat cgccagattt cagaccttcg ctaatcaacc atttatcgcc aatcgcctga
     8821 ctggcaacga cagggcgcgc ttccacctga cttttatcgt taacaatcag cacggttgca
     8881 tcaccacgcg gtgtgcggct aacgccttgt tgcgggataa gaatggcgtc aggttggacg
     8941 ccttcatcaa tccgtgcacg cacatacata cccggcaaaa gcgtatgttg cgggttaggg
     9001 aagacagcac gtagggttat ggagccggtg ctttcatcaa cggtcacatc agagaattgc
     9061 agcgtacctt tcaggggata ggtttgaccg ttttccatga ccaactctac gttgctggtg
     9121 gcgttttcct tatgcaaatt tccttgctct acggattgct tcagcctcat aaaatcgttg
     9181 ctggattggg tcacatcaac gtagatagga tcgagctgct ggacagtcgc cagttcagtc
     9241 gtttgcccat tagtgacaag agcgccttcg gtcacggtcg atttgccgat acgtccgcta
     9301 attggcgcag tcactttggt ataagcgaga ttgatgcgag cgctttcgac tgtgcctttt
     9361 gcggcaatca cggcggcatc ggcctggcga gcatcagcaa tggcctggtc gtactcctgc
     9421 tggctgatgt atttcgtacc cacgagcgga acgtaacgtt ttaccgtcaa atgcgcgatg
     9481 gcggcggcgg cttcactttt cgccagttcg cctttcgcgc tgttataatt tgcctgatag
     9541 gtcgcgggat cgatctggta cagggactgg cctgcttgta catcgctgcc ttcagtgaaa
     9601 ttacgattca gtacgatccc gctaacctgt gggcgaactt cggctatacg gtaagcattg
     9661 gtgcggcctg gtaattcagt cttaacttct aacggggccg ttttcacaat atgaacggta
     9721 acctgcggtt caccgacgtg agctttctct tctcccttat cgttacaacc ggcgattaaa
     9781 gccgcggaga tcagaataaa ggagggcagg aggaaaaacc tggcatgttt cgtcattact
     9841 attcctcaaa aaaccaaaag cgcgttattt acccagaaag gcaacgcgtt aactcgcaga
     9901 aagaaaaata cagtttgcta tcctacaaat tatcattcgt cgatgtaagg aatagttatg
     9961 aatactggcg tctcaaggca cataaacaca aaaaaagatt aatattctac tgttttattt
    10021 tgacgcgggt tgaaagaggc agaattaaaa cctcgtaaat tgaaatatat attcatgtgg
    10081 cgaatgtatt ttaggtaaat aatatacatt gtttatttga cgatttatag caactcaatg
    10141 ttaccctctc gtataaatac acattagatg atagattaac cttcgctatt ttctcaccct
    10201 gtgtcgaata tatttatttc ctgaataatt aatcatggca aaaagaacca aagccgaagc
    10261 tctgaagacc cggcaagaac tgattgaaac tgccatcgcc cagtttgcgc agcatggcgt
    10321 aagcaagacg acgctcaacg acattgccga cgccgctaac gttacgcgtg gcgctatcta
    10381 ctggcacttc gaaaacaaga ctcaactgtt taatgagatg tggttgcaac agccttcatt
    10441 gcgggagtta atccaggaac acttgacggc tggattagag catgacccgt ttcaacaatt
    10501 gcgtgaaaaa ttgattgtcg gcttgcaata tattgccaaa attccccgcc agcaggcgtt
    10561 gctgaaaatc ttatatcaca aatgtgaatt taatgatgag atgctggccg agggagtgat
    10621 acgcgaaaag atgggcttta atccgcagac tctccgcgaa gtattgcagg cgtgtcagca
    10681 acaaggttgt gtagcaaata acctcgattt agatgttgtg atgattatta ttaatggtgc
    10741 cttcagcgga attgttcaaa actggttaat gaatatggcg ggttatgatc tttataaaca
    10801 agcacccgcg ctggtcgata acgtattaag aatgttcatg ccagatgaaa acataacgaa
    10861 attaattcat caaacgaatg aattaagtgt catgtaaatt cacattctgt cctgaaaatt
    10921 cagttcgtca cagttaacca gttttagtgg attaactgag ttcatattac ggcatttgtc
    10981 tgtttctgtt gtcagcagtt caatccactg catcagtaaa gtatcaaaca gaaaaacgaa
    11041 gacagcgaaa acgacgagcc accaatactt gcgaatcatt acctgagcct gtgcatgttt
    11101 aaccaagtct ggcaggaaat atacacgaaa tgaataatct gaaaaccagg cttacacttc
    11161 ctttactttg caatgagatc ggggtcaact tcgcttaacc ggcttcgttt tgacaggttg
    11221 ccggtctttc tttttttcac tttcgccagt tcttccgcag agtaatgcga cgcgacatcc
    11281 agccgtcgaa gccccatttt gatgtactcg ctgttgatct caataccaat gaattttcgc
    11341 ccgctggaga cggctacggc accggtagta aagctaccgg caaacgggtc gagaatgata
    11401 tcgcctgggt tggaagaggc gagaataatg cgtttcagta aggcttccgg tttttgcgtc
    11461 gggtggttct catattcatc catcaaataa cgcacgcgcg gaaaatccca gacgttaccc
    11521 ggtacttttt gatgattgta tggctgtgga ggatttttgc gataatcgat caacgcgcgc
    11581 tgcgatccgg ttttggcttc taccagaata gcatcaccgt tgaatgtgta gttctttgcg
    11641 tctttcacca tcatcaggat ggggtcgtac atggagccgt agtgtttttt cgcctgtact
    11701 ccagaactgt catatgacca gacgatgcga cttttgatgg taaaaagctt gcggcactgg
    11761 agatcgataa agggcatgtt ttccgtgctg ttcatgatgt acatgctgcc ctgctttttc
    11821 agaacgcggt ggcactctgc aatcacttca aacagccagt cgataaacag atcttctttc
    11881 caggcttcga tcagaccatc aaaattttta ccgatgttat atggtgggtc ggcaaagatc
    11941 agatcgacac tttcggcggg gatcttttta agttcggcaa gcgcatcacc gtgaataatg
    12001 gtcttagctt cattaccaaa ccgggtcggt tcacatcctg ttctcatggt cactcccttt
    12061 gtgacaccta taaaaaaggc gcttccccat gccgagtagc gcctttttaa tcaagcattt
    12121 agctaacctg aattagttca tgccgtattt tttcaatttt ttacgcagcg taccacggtt
    12181 gatgcccatc atcagcgcag cacgggtctg gttaccacgg gtgtattgca tcaccatgtc
    12241 caacaggggc tgttctactt cagccagtac cagctcatag aggtcattca catcctgacc
    12301 attcagttga gcaaaatagt tcttcagtgc ctgtttaacc gagtcacgca ggggtttttg
    12361 ggttacctga tcctgagagt taacggtaga aacggtcagt acgtcagaat ttacgcgttg
    12421 ttcgaacata gttctgtcag ctctttattt ctgtttacgc aaaattttcg aagtatgcct
    12481 ccaacgcctc cagctgttcg ctggcatcct caatggcgtt gaatgtgcgc cgaaactggt
    12541 catttggagc gtgttcctgg agataccagg aaacgtgttt acgtgcaatt cggtaccctt
    12601 ttgccggacc ataaaagtca tgcagttccc gaacgtgcgc gcaaagcaag cgcttaacct
    12661 ctgccaaagg cagtgggggc agcaactccc cagtgtccag ataatgctgg atttcccgaa
    12721 agatccaggg tcttccctga gctgcgcggc ctatcatcag ggcatccgcc cctgtatagt
    12781 cgagcacagc tctggcttta agcgggtcag taatgtcgcc attcgcgata accggaatgg
    12841 aaactttctg cttaactgcc cgaatactgt cgtactcagc ttctccattg aacaaacagg
    12901 cgcgtgtacg gccatgaatg gtcagagcct gaatgccaca gtcttcagcc agttgggcaa
    12961 tctcttcgca gttacggtgt tccggtgccc agccagtgcg aatcttcagg gtaacaggaa
    13021 cgtccactgc attgacgacc tcggtaagga tcgatttaac gacatccggg tactgcaaga
    13081 gggctgaacc tgcgagcttg cgattcactt ttttagccgg gcaacccata ttgatatcaa
    13141 taatctgggc accgctttcc acgttaatac gtgctgcatc tgccatttct ttcggatcgc
    13201 taccagcaat ttgcacggtg cgaataccgg gttcatcaat gtgcaccatc cgtaaacgag
    13261 atttgtcgct ttcccaaacc tgtgggttag aagacatcat ctcggatact gtcaatccgg
    13321 ctcccatctc gtagcacaac gtccgaaaag gtctgtctgt aatgccagcc atgggcgctg
    13381 cgatcaggcg atttctgagc tgatattgtc cgatgcgcat gagttaagaa atgaccatac
    13441 tgtgactgca aggcggcgta tattacgcat tttttgcacg agatgaaagg ccaaactttg
    13501 accaatcctc tgagatggat caaagaattg catttaaaat gagcgtggtg cgataattac
    13561 tcataaaaat catcatatta gaaaatagtg actaaaattt acattcaaag aaatttgagt
    13621 aagttctcaa tttttcttta tgaatgaaaa ttttggcacg caaatttgcg taaataaccg
    13681 gcaaatttac gtgccttttg tgagcttgct cgcacttcgc cccgcgtcac cctacggcga
    13741 tgcgaaggtt aattcttacg accggtaata cggcaccact cttctttttc cacgaccggg
    13801 tccagtgcga agctatcggc ataagcttca caaacgctct ctgcctggct tgccagaata
    13861 ccggaaaggc ccagcaaacc gcctgaaacc ggcaggacgc tgattaacgg tgccagttca
    13921 cgtaatgggc ctgcaaggat gttcgcgacc accacgtcgg ctttcatttc ttctggctga
    13981 tctttcggta agtagagttc cagacggtca gaaacgccat tacgttcggc gttgtcgcgg
    14041 ctagcctgaa tcgcctgcgg atcgatatca ataccaatgg cttttgctgc acccagtttc
    14101 agcgccgcga tagccagaat gccggaaccg cagccaaagt cgatgactgt tttaccagtt
    14161 aaatcgaggc tgtcgagcca ttgtaggcac agagaggtag ttggatgggt acccgtacca
    14221 aacgccagcc ctgggtctaa catcacgttg acggcgtttt cgtccggcac atcacgccag
    14281 cttgggcaga tccacagtcg ttcaccaaag cgcatcgggt ggaaattatc catccattcg
    14341 cgctcccagt ctttatcttc cagttgttcg attttatgcg cgaagcctgc gccgagcagc
    14401 ggatggtttt ccagaatcgc caccacgtcg ttcatatcgg tttcagcgtc gaacagacca
    14461 atcacatcgg tgtcgcccca caggcgcgtt tcgcccggca gcggctcaaa tactggcgta
    14521 tcgtgggtat cctgaaaagt gatagaaacg gcacccgctt ccatcagcgc atcgctaaga
    14581 tcttcagcgt tcgcgccggt ggtgttcagt ttcagttgga tccaaggcat ggcaaaactc
    14641 tttatttatc agtagtcaaa acggtagctt gcgggacgga tgtaccgaaa cggtttccga
    14701 ccaggaaagc cagcaaactt agtagtaacg agggcacgat agggtggaag cccaggtact
    14761 gaatattcag cgtcgcgagt acggcataca gcacgccgcc aacgatcatc gcacttagcg
    14821 cgcctttggc gttggcgcgt tcccagtaaa gacccagcac cagcggccac aggaaaacgg
    14881 cttccaaccc accgaaggcc agcaaattca gccatatgat catttctggc ggcttccagg
    14941 cggcaagcag cagcaacgcg ccgagaacta acgtaattac cgccgacatc cgcttcagac
    15001 gcgtctcgtt ttgcatttga tccggacgga tattcagata gagatcttta atgatcgtag
    15061 cggaactttg tagcaattgg gcgttaattg tcgacatgat cgcagccata ggtgcagcca
    15121 gaaagatccc ggcagcaaac ggtggcagca cttttaccat taacgtcggg atcaccagat
    15181 ccggtacggt gagatcgggg atcactgcac gacctaacgc tccggccagg tgcataccga
    15241 acatcaggat tgcgactaca atcgtaccga tgatgatccc ccggtgtacc gctttgctgt
    15301 ctttataaga gatacagcgc accgcagtat gcggcaggcc aatcacgcca aagcacacca
    15361 gtacccagaa cgacgtcata aaggcaggcg acagaatatc gtcagcgcct tgtggcgtaa
    15421 ccagttgcgg atcgatggtt tgcagcgtct ctaccgcatt acttaaacca cctgcggcat
    15481 gtaccacgcc aataagcagc acaacggtgc caatcagcat cacaagccct tgcatggtgt
    15541 cgttcagcac gctggcgcga aagccgccaa aggcggtata caacgcaatg ctgataccga
    15601 aaatcagcag cccggtttca taaggaatac ccgccgcggt ttccagcagg cgcgcaccgc
    15661 cgataaactg cacggtcatt gccccaacga acgcaaccag caaactcaaa ctcgccagcc
    15721 acaccagaag acgactctgg tagcgggcaa acagcatgtc gttcagcgtc actgcattgt
    15781 agcggcgcgc aagaatcgcg aacttcttgc cgagaatacc gagcgaaagc cagactgcag
    15841 gaagctgaat catcgccagc aatacccaac ccaggccgta tttataagca gctccaggcc
    15901 cgccgataaa cgaacttgca ctgatatagg tcgcggtgag cgtcatcgcc agcacaatac
    15961 cgcccataga gcggctgccg aggaaatact cattaaggaa ggtaccggtg ctccgtttac
    16021 gcatcgcata aacagagata ccgaacacca ccaccagata ggcgaccagc ggtagaatca
    16081 cttcaagctg catcgtcatc ctccagtggg atatcgcgat agataaattt caccatcgcc
    16141 cagcacagtc caataaacag cagcggcgtc aggatgcagg ccatctcaaa ccagcgcgga
    16201 aagccggtaa aaccgggggc aacgtcaggt aagtaagcgg ctgctaacca aactgccaga
    16261 tacaaaaggg tcagccccag cgcccagcgc gcctctttat gggcctgaac aaaacgagtg
    16321 tccacggaag ggttcctcct catgggggga tacgagaaac gggcaattct acggcaactc
    16381 ttcaggcagg gaaaataatc tgacagaaaa ttgccgttca gtgctgctgc aaaaagtctt
    16441 caatgtcagc cagtgaggat atcccttcca tcggaccttg ccgcattacg gcgagcgcgc
    16501 cgctggcatt tgcatattgc agtgccttat gtgccgggaa accggataag aaaagagtga
    16561 taaacgttgc gccaaagcag tcgcctgcac ccgttggatc gataatttcg atatcgtggc
    16621 ctgtaacatg ctgggcgtgt aatgtaccgt ttttaagctt gtaatagctg gccccacgct
    16681 gggcgcgttt tatcgccaca tgttttacgc cgccgtggag aagatcgcta acaatctgtt
    16741 cttctgacag atttttgtga cgcgcgaaga aagggagttc gctttcgctg gggatgaaaa
    16801 tatccgtata ttcaatcaaa taatcgagag cctgcgccat ttcaggaatg ctcagcatct
    16861 ctttgcgaat attgggatcg aaagaaacgg tgccgccagc cgatttgatc gtcgttattg
    16921 ctttacgcat gacatcgatc atgcgaaatg agaacaatga tgagcccaca atatggagat
    16981 ggttacactg ttttagcaga tccttatcaa tgtgctcagc agtaaacaag ccgcaggcgc
    17041 tgttagggat attaaagacg aaatcgcgct gctggggatt ttgataactc acgaacgccg
    17101 tacctgtgac ggcattgttc ataacatgga tcccatcggt aatgacacct tcatgacgga
    17161 ggcgctctat aatgagtcgg ccaaaatcat cattaccaac acatccgaag aagatggccc
    17221 gatgggacag ttttgccacc tgagcagcaa aaattgctgg cgcaccgctg ggaaatggcc
    17281 cgataaactc cccaggctga gtgaattttt gatgtgggtt gcgggacaaa aactcggcca
    17341 gcaattcgcc catagcgcag atagttattg tcacaaaacc cctctcattc ctgaagcctt
    17401 tcgcgattgg accaggtaaa gcactcatta ctggtataaa ggataacgaa atcagccgac
    17461 tgcaattgtt gctgatattg ttcgttaatg ctgcggctga cgatcacatt ttctgcctga
    17521 tgcaaatgcc cgatagcagc cgttgatggc gcaggatcgg cggcaacgat aaccgtctta
    17581 ttctgggcat gatcgaaaca gagctgccag gcagcggcca gtctgggaac gttgatattt
    17641 aaagtaccat cagcatccag atggttgatt tccagaatgc tatgtgtaat ggtgaaatgc
    17701 tgaagtgcct gttcaagcgg ttttccggcc agtgcggtgt cggggcgcag catttcactg
    17761 ccagccagta ttatttcgcc atcaataaac tgatttgcca gcagcgcatg ttcgggatcg
    17821 ttggtgatca gacgcagttt tttcacttcg ctgagcaatg gaatgatctt acggcagatt
    17881 gtcccatgtc caagaaatag cgtgtcacga tcgcgaatta accgtgcgca atgtcgtgct
    17941 atttccattt gttgtgccag agacaatggc gcgatcggtt gtatcgcttc tggttcagag
    18001 gagagtggtg tcgcaatagc accgccaaat gatcgcttta aatatccctg ttgctccaga
    18061 taactgagat cgccgcggat cgtcacgcca gagacaccaa gttgcgcaga taagtcatca
    18121 actttgattt caccttgggt ggcaacaatt tctgcaattt ttaatctgcg tttcagtagg
    18181 ttagacgatt ttctcggcac gttaacactc ttcattggcc tttaatgcgt aacactatag
    18241 ccccttgtat ttttctttga aagttatcct tcaataatat cgacaattcg aaaagaaaat
    18301 catgggtcaa aaatcgcaat aaaaatagat taattcattg aaaaataaat gaatttattc
    18361 atatttatgc agagatgtta ttggtttcac ttgtaaagtg aaaaacaaat taagttattt
    18421 ttttgtaagt tattttgatg atattcgaca tgtatcacgc tttttgccgc ttttgctgtg
    18481 ctagtttgct ttcaaaatga aagtgaatat tctctttctt cattttgtaa ggcgaacgac
    18541 aaataacagg agctgcgatg tacatcattt caagcaaaaa catgctgaaa aaagcccagt
    18601 cagagggata tgccgtcccc gctttcaaca ttcataacct tgaaacttta caggtagttg
    18661 ttgagagcgc tgcacaactt cgctctcccg ttatgctggc gggaacaccc ggtacttatc
    18721 gttatggtgg cgtgggaagt ttaatttcgt tagtacaatc gctggctcgc gagtacaacc
    18781 tgccgttagt cctgcatctc gaccatcatg aagagagtga tgacattttt aacaaggttc
    18841 gcgcagggat ccgttctgtc atgattgatg gctcccattt gccatttgaa gacaacattg
    18901 cgcgagtgca agaagttacc accttttgcc atcgctttga tgttagcgtt gaggccgagc
    18961 tagggcgtct tggcggccag gaggacgatc tgctggtgga tgaaaaagat tccgcttata
    19021 cctcaccagc cgcagctgca gagtttgttc aacgtaccca aattgactcc ctggccgtag
    19081 caatcggcac agcacatggt ttatatgcgc atgaaccacg actggatttt gtgcggctgg
    19141 aacagattcg tcagcgagtg gagattccac tggtgttgca tggtgcttcg ggcttatcag
    19201 aacaggacgt tcagcactgt attcgccgcg gtatttgtaa agtgaatgtc gcgacagaat
    19261 tgaaaattgc gtttgccgat gcgttaaaag gatatttcta caacaatcct gcggcaaatg
    19321 atccacgcca ctatatgcag cccgcaaagg ccgccatgaa agaagtcgta actcgtatta
    19381 ttggtgtctg tggtagtgaa ggaaaaattt aagacgttgt accctacaaa aacaatgagg
    19441 attgtttatg aaaaatatta aatta
//
LOCUS       DAJVUJ010000073        19401 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.073, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000073 DAJVUJ010000000
VERSION     DAJVUJ010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19401
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.073"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(31..1860)
                     /gene="glmS"
                     /locus_tag="OQQ48_002293"
     CDS             complement(31..1860)
                     /gene="glmS"
                     /locus_tag="OQQ48_002293"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCS1953047.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIMVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(2022..3392)
                     /gene="glmU"
                     /locus_tag="OQQ48_002294"
     CDS             complement(2022..3392)
                     /gene="glmU"
                     /locus_tag="OQQ48_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCS1953048.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILISNGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDVNLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(3743..4162)
                     /gene="atpC"
                     /locus_tag="OQQ48_002295"
     CDS             complement(3743..4162)
                     /gene="atpC"
                     /locus_tag="OQQ48_002295"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCS1953049.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQSGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(4183..5565)
                     /gene="atpD"
                     /locus_tag="OQQ48_002296"
     CDS             complement(4183..5565)
                     /gene="atpD"
                     /locus_tag="OQQ48_002296"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCS1953050.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5592..6455)
                     /gene="atpG"
                     /locus_tag="OQQ48_002297"
     CDS             complement(5592..6455)
                     /gene="atpG"
                     /locus_tag="OQQ48_002297"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCS1953051.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHLYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6506..8047)
                     /gene="atpA"
                     /locus_tag="OQQ48_002298"
     CDS             complement(6506..8047)
                     /gene="atpA"
                     /locus_tag="OQQ48_002298"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCS1953052.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(8060..8593)
                     /gene="atpH"
                     /locus_tag="OQQ48_002299"
     CDS             complement(8060..8593)
                     /gene="atpH"
                     /locus_tag="OQQ48_002299"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCS1953053.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8608..9078)
                     /gene="atpF"
                     /locus_tag="OQQ48_002300"
     CDS             complement(8608..9078)
                     /gene="atpF"
                     /locus_tag="OQQ48_002300"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCS1953054.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(9140..9379)
                     /gene="atpE"
                     /locus_tag="OQQ48_002301"
     CDS             complement(9140..9379)
                     /gene="atpE"
                     /locus_tag="OQQ48_002301"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HCS1953055.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9426..10241)
                     /gene="atpB"
                     /locus_tag="OQQ48_002302"
     CDS             complement(9426..10241)
                     /gene="atpB"
                     /locus_tag="OQQ48_002302"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCS1953056.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10250..10630)
                     /gene="atpI"
                     /locus_tag="OQQ48_002303"
     CDS             complement(10250..10630)
                     /gene="atpI"
                     /locus_tag="OQQ48_002303"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HCS1953057.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(11247..11870)
                     /gene="rsmG"
                     /locus_tag="OQQ48_002304"
     CDS             complement(11247..11870)
                     /gene="rsmG"
                     /locus_tag="OQQ48_002304"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCS1953058.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(11934..13823)
                     /gene="mnmG"
                     /locus_tag="OQQ48_002305"
     CDS             complement(11934..13823)
                     /gene="mnmG"
                     /locus_tag="OQQ48_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCS1953059.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(14202..14645)
                     /gene="mioC"
                     /locus_tag="OQQ48_002306"
     CDS             complement(14202..14645)
                     /gene="mioC"
                     /locus_tag="OQQ48_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HCS1953060.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(14735..15193)
                     /gene="asnC"
                     /locus_tag="OQQ48_002307"
     CDS             complement(14735..15193)
                     /gene="asnC"
                     /locus_tag="OQQ48_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HCS1953061.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQHPIMRTIKP"
     gene            15345..16337
                     /gene="asnA"
                     /locus_tag="OQQ48_002308"
     CDS             15345..16337
                     /gene="asnA"
                     /locus_tag="OQQ48_002308"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HCS1953062.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVIHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYLDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            complement(16342..17793)
                     /gene="viaA"
                     /locus_tag="OQQ48_002309"
     CDS             complement(16342..17793)
                     /gene="viaA"
                     /locus_tag="OQQ48_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HCS1953063.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWSEALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(17787..19283)
                     /gene="ravA"
                     /locus_tag="OQQ48_002310"
     CDS             complement(17787..19283)
                     /gene="ravA"
                     /locus_tag="OQQ48_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HCS1953064.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
BASE COUNT         4638 a         5124 c         4861 g         4778 t
ORIGIN      
        1 tcggcagccc ttttttatct atgatagtga ttactcaacc gtaaccgatt ttgccaagtt
       61 acgcggctgg tcaacgtcgg tgcctttgat cagcgcgaca tggtaagcca gcagctgcag
      121 cggaacggtg tagaagatcg gtgcaatcac ctcttccaca tgcggcatct cgatgatgtg
      181 catgttatcg ctacttacaa aacccgcatc ctgatcggcg aagacataca actgaccgcc
      241 acgcgcgcga acttcttcaa tgttggattt cagtttttcc aacagttcgt tgttcggtgc
      301 aacgacgata accggcatat ccgcgtcaat cagcgccagc gggccgtgtt tcagctcgcc
      361 agcagcgtag gcttcagcgt gaatgtagga gatctctttc agcttcaatg cgccttccag
      421 tgcgattggg tactgatcgc cacggcccag gaacagtgcg tgatgtttgt cagagaaatc
      481 ttctgccaga gcttcaatgc gtttgtcctg agatagcatc tgctcaatac ggctcggcag
      541 cgcctgcaga ccatgcacaa tgtcatgttc aatggaggca tccagacctt tcaggcgaga
      601 cagcttcgcc accagcatca acagcacagt taactgagtg gtgaatgctt tagtggacgc
      661 cacgccgatt tccgtacccg cgttggtcat cagcgccaga tcggattcgc gcaccagaga
      721 agaacccgga acgttacaga ttgccagcga accaaggtaa cccagctctt tcgacagacg
      781 caggcccgcc agggtatccg cggtttcgcc agactgtgac aaggtgatca tcaggctgtt
      841 acgacgcacg gcagatttac gatagcggaa ttcagaggcg atttcgacgt cgcacggaat
      901 acctgccagc gattcaaacc agtagcggga aaccataccg gagttataag aagtaccaca
      961 ggcgaggatc tgaatatgct caaccttcga cagcagttcg tcggcgttcg gtcccagctc
     1021 gcttaaatca acctgaccgt ggctgatgcg cccagtaagg gtgtttttga tcgcgttcgg
     1081 ctgttcgtag atctctttct gcatgtagtg acggtaaata cctttatcgc ccgcgtcata
     1141 ttgcagattg gattcgatat cctgacgttt tacttccgcg ccagttttat cgaagatgtt
     1201 taccgaacgg cgagtaattt ccgcaatatc gccctcttca aggaagataa agcgacgggt
     1261 caccggcagc agcgccagct ggtcagaagc gataaagttt tcgcccatac ccaggccaat
     1321 caccagcgga ctaccagaac gtgccgccag cagggtatcc gggtgacggg agtccatgat
     1381 cactgtaccg tacgcaccgc gcagctgcgg gatagcacgc agaacagcct cacgcagagt
     1441 cccgccttgt tttagctccc agttcaccag atgggcaatc acttcggtgt cggtttcaga
     1501 aacgaaggta tagccacgcg cttttaactc ttcacgcagc ggttcatggt tttcgatgat
     1561 gccgttatgc accaccataa tgtgttcaga aacatgcgga tgcgcgttag cttctgaagg
     1621 ttcaccgtgg gtcgcccagc gagtgtgagc aataccggtg ccgccatgca gaggatgttc
     1681 ttccgctgcc tgagccagca tctggacttt accgaggcga cgcaggcggg tcatatgacc
     1741 ttccgcatca acaacggcca gaccagcaga gtcatatccg cggtattcca gacgacgtaa
     1801 accttcaaga aggatttctg ctacatcacg ttgcgcgatc gcgccaacaa ttccacacat
     1861 agtttttgat tccgatttat atcgttgtcg gtcaacctgt atgcccgttt tttcgggcgc
     1921 cccgagcctt gtagagagtg gggttatttt tatagttact gcttgtggga gggagattat
     1981 gttatctcct catcccagat gaccggatta cccggccaga attacttttt ctttaccgga
     2041 cgacgccagc cttctttctg agtctgcggc acacggctga tagctaatgc attttcgccg
     2101 acattacgcg tcacagttgt acccgcagca atggtcgcgc ctttgcctac tgttaccggg
     2161 gccaccagct gagtgtcgga accgacaaac acatcgtcgc cgataatggt cttaaattta
     2221 ttcgcaccat cgtagttgca ggtaatggtt cccgcgccga tgttaacgtt atcgccaatt
     2281 tccgcatcgc ccaggtaagt cagatgacca gctttcgagc ctttacccag acgcgctttt
     2341 ttcatctcaa caaagttacc gacgtgtgca ccttccagca actcagcacc aggacgcaga
     2401 cgggcaaacg ggccaatagt acaggccgct gccagattca cgtcttccac aacggtatac
     2461 ggactgattt cgcaatcatc gccaatcacg ctgtttttaa tcacgcaacc ggtgccgatt
     2521 ttcacgcgat gaccgagagt cacgttgccc tcgatgataa cgttagtatc aatttcaaca
     2581 tcgcgcccgt gagtaagcgt accgcgcaga tcaaaacgcg ccggatcgcg cagcataacg
     2641 cctgctaaca gcagtttttc agcctgttcg gactggtaaa cgcgctccag acgggagagt
     2701 tgcaggcggt tattcacacc ttctacttcg cttaaacgtt gcggatgaac ggcgacgatt
     2761 tcacgccctt cctgatacgc cagcgcaata atgtcggtga tgtagtattc gccctgagca
     2821 ttattgttgg tcagcttcgc cagccagcgt ttcatatctg cgccgttaga gatcagaatg
     2881 ccggtgttga tctcctgaat ctgacgctgc tcgtcggtgg catctttgtg ctcaacaatg
     2941 ccggtaactt tgccattttc acgggtgata cgtccataac cggtcggatc atccagtttc
     3001 accgtcagca gaccaatgcc accctgcggt ttagcatcac gcaggcgctg gagtgtttcg
     3061 acagagatca gcggcacgtc gccgtagagc attaaaatgt cttcatcatc ggcaaagaaa
     3121 ggtgcggcct gctgcattgc atgacccgta cccagctgct ctgcctgaag cacccagtta
     3181 aggttgtcgt ctttcagcgc ctgtttaagc agatcgccgc cgtgaccgta caccaggtga
     3241 acgtgcgctg cgcctaattc attcgcagca tcaatgacat gctgaaccat cgctttcccg
     3301 gcaagggtat gcagcacttt cggaagatcg gaatacatgc gcgtgccttt gcctgcggca
     3361 aggatcacta cgctcatagc attattcaac atacgcgtcc tgactgtaat ttgagaacga
     3421 atttaaaccg cttcaccttg aaaaaactac atttttttca tcgtgaaatg gacagaggat
     3481 aaattgttca ataacggatt atccccgctg acgaaagtgc cattttcgac catcgtcacg
     3541 ccttttgtct gcagaaaatt aaggcagtac cttcatttaa gcgtcaggtg gatgtttttg
     3601 ctcttatttc gatcaatgaa taaacgggaa aacaggggtg ttttatatca gcaggatcta
     3661 tgtgaacgct cttcaggacg gatcacgcgt aaaaaaaaac cagcctgttt ccagactggc
     3721 ttttgtgctt ttcaagccgg tgttacatcg cttttttggt caactcgata acgcgcagct
     3781 gcgcgatcgc tttggccagt tccgcagacg cctgagcgta atctacgtcg ccgtgagagc
     3841 tgctaatgtg ctcttcagcc ttacgtttcg cttccatggc tcgcgcttcg tcgagatcct
     3901 ggccgcgaat tgcggtgtcg gccagaacgg tcacgttgcc agactgcact tcaagaatgc
     3961 cgccagacag atagataaac tcttcgtgac cgtgctgttt cacgatgcga atcataccag
     4021 gcttaatggc ggtgagcagc ggtgcgtggc cagggtagat ccccagttca ccttcgctac
     4081 ccgttacctg gattttctcg accagaccag agaacatttg ttgctctgcg ctgacgacgt
     4141 ccaggtggta agtcattgcc atatcaccct ccgattaagg cgttaaagtt ttttggcttt
     4201 ttccacagct tcttcgatgg aaccgaccat gtagaacgcc tgctccggca ggtgatcgta
     4261 ttcgccttcc atgatgcctt taaagccacg gatggtgtct ttcagggaga cgtatttacc
     4321 cggagaaccg gtgaatactt ctgccacgaa gaacggctgg gacaggaagc gctggatctt
     4381 acgagcacgc gctaccacca gtttgtcttc ttcagacagt tcatccatac ccaggatggc
     4441 gatgatgtct ttcagttcct gataacgttg caggatggac tgaacgccac gcgcggtgtc
     4501 gtagtgttcc tgaccaacca ccagcgggtc cagctgacgg ctggtggagt ccagcgggtc
     4561 aacggccggg tagataccca gagacgcgat ctgacggctc agtaccacgg ttgcgtcaag
     4621 gtgcgcaaag gtggttgccg gagacgggtc agtcaagtca tccgcaggta cgtataccgc
     4681 ctgtacggag gtgatagaac cagttttggt ggaggtgata cgttcctgca gaacgcccat
     4741 ctcttccgcc agggtcggct gataacctac cgctgaaggc atacggccca gcagtgcgga
     4801 tacttccgta ccggccaggg tgtaacgata gatgttgtca acgaacagca gaacgtcacg
     4861 accttcgtca cggaatttct cagccatggt cagaccggtc agagcaacgc gcagacggtt
     4921 tcccggcggc tcgttcatct ggccatacac cagggatact ttgtcgataa cgttggagtc
     4981 ggtcatttcg tggtagaagt cgttaccctc acgagtacgt tcacctacgc ccgcaaacac
     5041 agagtaaccg gagtgctcga tcgcgatgtt acgaatgagc tccatcatgt ttacggtttt
     5101 acctacaccc gcaccaccga acagaccaac tttaccgccc ttagcgaacg gacacatcag
     5161 gtcgataact ttgataccgg tttccagcag ttcctgagag tttgacagct cttcgtagga
     5221 aggtgctgcg cggtgaatcg cccaacgctc ttcttcaccg atctcgcctt tcatgtcgac
     5281 cggttcaccc agtacgttca tgatacggcc cagagtcgct ttacctaccg ggacttcgat
     5341 cgggtgttcg aggtctttta catccagacc gcgacgcaga ccgtcggagg aacccattgc
     5401 gatggtacgc acgataccgc cgccgagctg ctgctgaact tccagcacca gacgctcatt
     5461 accattttgc acctcaagag catcgtacac gcgcggtacg gcatcctgag ggaattcgac
     5521 gtcaactacg gcgccgatta cctggacaat ctttccagta gccatcttaa atcctctacg
     5581 aaataacctg tttaaaccgc ggcggccccc gagacgatct cggtgagttc ctgagtaatg
     5641 ctggcctgac gagctttgtt gtataccaac tgcagctctt taatcaggct gccgccattg
     5701 tcggtcgcgg ctttcatcgc caccatacgg gcggcctgct cgctggccag gttttcaacc
     5761 acgccctgat aaacctgaga ttcgacataa cgacgcagca gggtatccag caacgccttc
     5821 ggatcgggtt cgtacaggta atcccaggat ttatgtttca gatcatcatc atctgatgcc
     5881 ggtaacggca gcagctggct gatggtcgga acctgagaca tggtgttaat aaatttgttg
     5941 ctgacaatgt aaagcttgtc cagacggcct tcgtcgtagg cctgcaacat cacttttacc
     6001 ggaccgatca gttcggacag ggaagggtta tcccccatgc cggtgacctg ggcaacaaca
     6061 ttgccgccca cggagttgaa gaacgacacg cctttcgagc cgatcattgc gaggtcgcat
     6121 tgaacgcctt tgtcggtcca ggtcttcatt tccgccagca gttttttgaa caggttaatg
     6181 ttcaaaccac cgcacaaacc acggtcggtc gacaccacca ggtagcccac gcgtttaacg
     6241 tcgcggtctt ccaggtaaag gtgcttatat tccagattac catgtgcaag gtgaccaatc
     6301 actttgcgca tggtttctgc ataaggacgg ctggccgcca tgcgatcctg cgatttacgc
     6361 attttggaag cggcgaccat ctccatcgct ttagtgatct tttgcgtgtt ctggacgctt
     6421 gcgatcttac tacgtatctc ttttgcgccg gccatgagct tctcctcaat gccttgcggc
     6481 ctgccctaag gcaagccgcc agacgttacc aggattgggt tgctttgaag gaatcgagga
     6541 tgcctttcag tttgccttcg atttcgtcgt tgtagccacc ggtctggttg atctcttgca
     6601 tcaacggagc gtgatcacgg tcgacgtaag ccagcagagc ggcttcgaag ctgccaattt
     6661 tcgacagttc aacatccgcc aggtaaccac gttctgctgc gaacagaacc agagactgct
     6721 gcgcaacgga catcggcgca tactgtttct gtttcagcag ttcggtcact ttctgaccgt
     6781 ggtcaagctg cttacgtgtt gcatcgtcaa ggtcggatgc aaactgagag aacgctgcca
     6841 gttcacgata ctgtgccaga gcggtacgga taccaccgga cagttttttc atgatcttgg
     6901 tctgtgctgc accaccaaca cgggatacgg aaatacccgg gttaaccgca ggacgaatac
     6961 cggcgttgaa caggttggtt tccaggaaga tctgaccatc ggtaatggag attacgttgg
     7021 tcggaacgaa cgcagaaacg tcacccgcct gagtttcgat aatcggcagt gcggtcagag
     7081 aaccggtttt ccctttcact tcacctttgg tgaaggcttc aacgtattcg gcgttaacac
     7141 gtgcagcacg ctccagcaga cgagagtgga ggtagaaaac gtcgcccggg aatgcttcac
     7201 gtcctggcgg acgacggagc agcagggaga tctgacggta agcaacagcc tgtttagaca
     7261 ggtcatcgta aatgatcagc gcatcttcac cgcggtcacg gaagtattcg cccattgcgc
     7321 aaccggcata cggtgccagg tattgcagtg cagcggattc agacgcggtt gctaccacaa
     7381 cgatggtgtt agccagtgcg ccgtgctctt ccagtttacg taccacgtta gaaatggtgg
     7441 acgctttctg gccgatagcg acatagatac atttgatacc ggaatcgcgc tggttgatga
     7501 tggcatcgat agccagtgcg gttttacctg tctgacggtc accgatgatc aattcacgct
     7561 gaccacgacc gattgggatc atggagtcaa cggctttata accggtctgt accggctgat
     7621 ctacggactg acgttcgata acgcccggag cgattgcttc tacagcagag aagccgtcgt
     7681 gatccagcgg acctttaccg tcgattggtg cacccagagt gttaaccaca cggcccagca
     7741 ggccacggcc aaccggaact tccaggatac ggccagtaca cttaactttc atgccttcgg
     7801 caaggtcagc gtacggaccc ataacaaccg cacctacaga gtcgcgctcg aggttcagtg
     7861 cgatagcgta acggttaccc ggcagggaga tcatttcacc ctgcatacaa tcggccaggc
     7921 cgtgaatgcg gataacaccg tcacttacag aaacaatagt accttcgttg tgagcttcac
     7981 tcacaacatt gaactgagca atgcgctgct tgatcagttc gctgatttcg gtggaattca
     8041 gttgcatgct ccagtcccct taagactgca agacgtctgc aaggcgctca agacgaccgc
     8101 gtacgctgcc atcaatgacc atatcacccg ctcggatgat aacgcctgcc attacagact
     8161 tatcgatttt gcaattcagc ttaactttgc gtgacagacg tttttccatc gcagcagaaa
     8221 ttttcgcgag ctgttgttca ctcagtgcgg cagcggaaat gacgtctacc tcagcggtag
     8281 cctcactcac ggcacgaagg tgaataaact gctccagaac atccgggagc gcgttaagac
     8341 gaccattttc agccattacc cgaatcaggt tctgaccgtt ttcgtccagt tgctcaccac
     8401 aaactgcgat aaacgactcg gcgagcgttt ctggcgcaag cgcgccagag agaagctctg
     8461 ccatttgttc gtttttggtt acctcggcgg caaacgccag catgtcctgc cagcgttcta
     8521 cactttggtg ttcgacggca aagtcaaaag ctgctttggc gtaggggcga gctaccgtaa
     8581 taaattcaga catcagcccc tccctcctta cagttcagcg acaagtttat ccacgatgtc
     8641 gctgttagca gcttcatcca cggaacgttc gatgatcttc tcggcgccag caacagccag
     8701 gatagcaact tgcttacgca gctcttcacg ggcacgttta cgctcggctt caatttctgc
     8761 ctgcgcctgg gccacgattt tagtacgttc ctgttctgcc tcagctttcg cttcgtccag
     8821 aatctgcgag cggcgtttgt tcgcctgctc gatgattacc tgggcttccg ctttcgcttt
     8881 tttcagctgg tcggtcgcgc tggcctttgc aaggtcaagg tccttatgtg ctcgttctgc
     8941 ggaagcaagg ccgtcagcaa tttctttttg acgtttttcg atggctgcca ttaatggcgg
     9001 ccatacgtac ttcatgcaga acagaacgaa caggacaaac gcgatggcct ggccgaggat
     9061 tgttgcgtta agattcacag cacaatgcct ctatttagtt aacgttctga tattgctctt
     9121 taaataaaag caacgcttac tacgcgacag cgaacatcac gtacagaccc agacctacag
     9181 cgatcatcgg gatagcatcc accagaccca taacgataaa gaactgagta cgcagcagag
     9241 gaatcagatc aggttgacgc gctgcgcctt ccaagaattt acccccgagg atgccgatac
     9301 cgatcgcagc accgattgcc gccagaccca tcatcacagc ggcagccatg tacagcagat
     9361 ccatattcag gttttccatg acagtctcca gtttgtttca gttaaaacgt agtagtgttg
     9421 gtaaattaat gttcttcaga cgccatcgac agatagacga tcgtcagaac catgaagatg
     9481 aaggcttgca gcgtaatgat caggatgtgg aaaatggccc acggcacatt caggatccac
     9541 tgtgaccacc acggcaacag accagcaatc agaatgaaaa tcagctcacc ggcatacatg
     9601 ttaccgaaca gtcgcaaacc gagtgaaact ggtttggaca gcaggcttac cccttcaagg
     9661 attaagttga caggaatgaa cgcccagtga ttgaacggct gcagcgtcaa ctctttcgtg
     9721 aagccgccga tgcctttcat tttgatgctg tagaacagaa tcaggataaa tacgcccaat
     9781 gccatagaca gcgttacgtt cacgtccgca gacggaacca cacgcagtgc aggcagaccc
     9841 agtacatgtt cagcaatgta cggcagcagg tcgataggca gtaaatccat caggttcatc
     9901 aggaataccc agacgaagat cgtcagggcc agcggagcaa tcagcttgct tttgccatgg
     9961 tacatgtctt tcacgctacc attaacaaag ccgatcacca gctcaatcgc ggtctgaaac
    10021 ttacctggca caccgctggt cgcctttttg gctacgctac ggaataaaac caggaacaac
    10081 agacccagca ccaccgagaa gaacatggag tcaatattga ttgtccagaa ggtggctggg
    10141 gggttttgtg gatccaccag cgagaatgta cgcaggtcca gctgaaggtt attcaggtgg
    10201 tgtcctatgt aatcctgcgg cgtcatattt tctgaagcca tgatgccttt taccctttgt
    10261 tgttaattac agccggtgcc agtatctgaa ccaccagcac caaaacccac gtaacgatca
    10321 gcggcaagaa taccgccttt aaaaccgcca acgccaccac cagtaacacc aacatcgcca
    10381 gaactttgaa agcttcgcca aatgcgaatg tccaggccac ccggcctttc gctggtgtat
    10441 gcgcctggtg acgccaggca aatatcataa acaaaacgtt aggcagaaag actgccaggc
    10501 ccccgcttat tgcagagacg ccccagaagg ggtctttgag gctgaacagc aatccacttg
    10561 ctatcaccac cagtaactga acgagcagaa gcttccgagc aacgtttcga ctcacgagcg
    10621 acacagacat cacgtttttt actcctgctc ccttcgaggt atgccgcgtg tcgtataaaa
    10681 ctttctttaa ggcttagagt caagcatcaa aaagcggtca aattatacgg cgcgcccccg
    10741 tgatttcaaa caataagtag ccaaaaggtg aataaatgtt taaatatttt tccagtgcat
    10801 acaattgcga cttttctact aaccctgttc gatcatgaaa aactgtaaat aacgcgtaaa
    10861 cactggtgat aaagcgtgct tcagatcaca tattgcgcat gttcgcgcac agcatattta
    10921 tttacttggc aaatgatgcc tttgcaagtt tatgatattt cagtctaaaa acagatactg
    10981 ttttaataaa tgacatttac acaacaaaaa ccacccattg acatttttaa taatgtttta
    11041 gcagccaatg atggttctta gcgccgattt ttagcagact gatattttca ctaatgactt
    11101 attttctgct taccaaaaaa agccacgtta tcttgttgat gcaaaagagt gaacgtggcg
    11161 ttaaatgtaa ccagttatat cagtagaaaa cctggttgtt gttaacagtc taaccggtca
    11221 attttttatg atttttttga taaaaattaa attttatttg ctttaatcac caccagatga
    11281 cgttcgccat ccagggctgg aacctgaagt ttaaccactg attcgacctg atattcttcg
    11341 ggcaacaaag cgatttcatc ttccggcatt tgccctttca gcgcgtagaa acggccttgc
    11401 tcaccaggaa gatggtggca ccagctcacc atatcgttca gagaggcaaa agcgcggcta
    11461 attacgccat caaatggcgg ctctgaagga aactcttcta ccctgctctg tacaggttca
    11521 atattctcca gtttaagctc atgttgcacc tgacgaagga aacgcacgcg tttaccaagg
    11581 ctatccaaca gagtgaaatg ggcttcagga cgcacgatag agagtggaat gcctggcagt
    11641 cctggtccgg tgccgacatc gataaaccgt tcaccttgca gatacggtgc caccacaatg
    11701 ctatcgagaa tatggcgtac cagcatctca ttaggatcgc ggaccgaagt caggttgtac
    11761 gctttgttcc atttatgcag catattcacg taggcaataa gctggttttt ctggtgatcg
    11821 gtaagcgaaa tacctgcgtc tttcagcagt aaggagagtt tgttgagcac ggtgattacc
    11881 tgttcttgat gcgttgcctg gtaagcgggt gcttaccagg catttttaat gcgttatgcg
    11941 ctacgacgca gcataccctg ttttttcagc cacaccagca gaatggagat agccgcaggc
    12001 gtgacgccag aaatacgcga agcttggccg atagaggctg gtttgtgatc gttaagtttg
    12061 gcgatcactt cgttagaaag accggatacc tggcggtaat ccagtgtcgc gggtagcagg
    12121 gtgttctcgt tacgcagctg cttttcgatc tcatcttgct ggcgcgcgat ataaccttcg
    12181 tatttaacct gaatctcaac ctgttccgcc gcctgttcgt ctgtcaacgc aggggcaaac
    12241 ggcgtcagcg tggttaattt ttcataagtc atttccggac gacgcagcag atcttcacca
    12301 ctggcttcac gggaaagcgg cgcagtcagg tgagcattca cttcggctgc agcttccgcc
    12361 gacggggtta cccaggtcga tttcagacgc tgacgctcac gctcgatatt ctcaagtttc
    12421 tcgttaaagc gcgcccaacg ttcgtcatcc accaggccca gttcacgacc gatttcagtc
    12481 aaacgcagat ccgcattatc ttcgcgtagc atcagacgat attctgcgcg cgaagtaaac
    12541 atacgatacg gttctttggt tcctaaagtg cacaggtcat caactagtac gccgagatac
    12601 gcctgagaac gtgccggagc ccaaccttct ttgtcagcag acagacgggc agcgttaaga
    12661 ccggccagca aaccttgcgc agcggcttct tcgtaaccgg tagtgccgtt aatctgacca
    12721 gcaaagaaca gcccctggat aaacttgctc tccagcgtcg gtttcaggtc gcgaggatcg
    12781 aagaagtcat actcaatggc ataacccgga cgcacgatct tcgcgttttc catcccctgc
    12841 atagagcgga cgatttgcat ctgcacatcg aacggcaggc tggtggagat accgttcgga
    12901 taaatttcat tagaggtcag tccttccggt tcaaggaaga tctgatgctg atttctgtcg
    12961 gcgaagcgca tgactttgtc ttcgatcgac gggcagtagc gtgggccgac accttcgatc
    13021 acccctgcat acattgggct acgatcgagg ttactgcgga tcacatcatg ggttttctcg
    13081 ttggtatgag tgatataaca cggcacctgc tggggatgct gggacgcatt gcccataaac
    13141 gagaataccg gcattgggtt atcgccatgc tgttgcgcca gcacactaaa gtcgatagtt
    13201 cgagcatcaa tacgcggtgg tgtcccggtt ttcagacgac caacgcgcag cggcagttca
    13261 cgcaaacggc gagaaagcgg aatggacggc ggatcaccag cacggccacc gctgtaatta
    13321 tccagaccga tatgaatttt accgtcgagg aacgtcccaa cggtgagcac gacggctttg
    13381 gcacggaact tcagtcccat ttgggtaaca gcaccgacca cgcgatcgtt ttcgacaata
    13441 agatcttcaa ccgcctgctg gaagatcatc aggttcggtt ggttctccag cgccgtacgt
    13501 accgcctgac ggtagagcac acgatccgcc tgagctcggg tagcgcgaac cgccggtcct
    13561 ttgcttgcgt ttagtatcct aaactggata cccgcttgat cgatcgcttt cgccatcaga
    13621 ccgccgagtg catccacttc ttttaccaga tgtcccttcc caataccgcc gatcgccggg
    13681 ttgcagctca tctgccccag agtgtcgata ttgtgtgtca aaagcagagt ctgttgaccc
    13741 atacgcgccg cggccatcgc ggcctcggtg cctgcatgac ccccgccaat gatgatgacg
    13801 tcaaaaggat ccggataaaa catggtgatt gcctcgcata acgcggtatg aaaatggatt
    13861 gaagcccggg ccgtggattc tactcaactt tgtcggcttg agaaagacct gggatcctgg
    13921 gtattaaaaa ggagatctat ttatttagag atctgttcta ttgtgatctc ttattaggat
    13981 cgcactgccc tgtggataac aaggatccgg cttttaagat caacaacctg gaaaggatca
    14041 ttaactgtga atgatcggtg atcctggacc gtataagctg ggatcagaat gaggggttat
    14101 acacaactca aaaactgaac aaccgttgtt ccttggataa ctaccggttg aaccaagctt
    14161 cctgacagag ttatccacag tagaacgcac gatctgtata cttatttgag taaattaatc
    14221 cacgatccca gccattcttc tgccggatct tccggaatgt cgtgatcaag aatgttgatc
    14281 ttcagtgttt cgcctgtctg ttttgcaccg gaatttttga gttctgcctc gagtttatcg
    14341 atagccccac aaaaggtgtc atattcacga ctaccaatac cgattgcgcc aaagcggacc
    14401 gcagaaagat cgggcttctg ttcctgcaat gcttcataga aaggagaaag gttgtccgga
    14461 atatctccgg caccgtgggt ggagctgata atcagccaga tccccgaggc agataaatct
    14521 tccaacagcg gaccgtgcag cgtttcggtg gtaaaacccg cctcttccag cttttcagcc
    14581 aggtgttctg ctacatattc ggcaccgccg agggtgctgc cgctgataag agtgatatct
    14641 gccataaacc gccaccttta ttaagagtgg cgtattgtac gctgtgaacg cgttgggatc
    14701 tacctgtgga aaagtatggg attaaaaaag ccgatcaggg cttgatagta cgcatgatcg
    14761 ggtgttgcag gacgatcaat gtctcggtgg actgaatttc atcaattgtt tggatcttgt
    14821 tgataagtac atgctggaga gcgtcgatcg aacggcacat tacttttata aagatgctgt
    14881 agtggcctgt tgtgtaatag gcttcagtga cttcatcaag gctttccagc tttgccagcg
    14941 cggaagggta gtctttggcg ctctttaata taatgccgat aaagcagcct acgtcataac
    15001 cgagctgctt cgggctgaca tcaatacgcg ccccggtaat gatccccgcc tgcttcattt
    15061 tctctacccg aacgtgaatc gtccccggac tgacgccaaa ttgtttcgcc agttcggcgt
    15121 aagcggtgcg cgcattgccc attaatgctt ccaggatgcc acggtccaga ttgtcgatca
    15181 gataattttc cataggattt tcttatgcag attgatgatt cattctattt tagccttctt
    15241 ttttaatgaa tcaaaagtgt gttaggcttt ttattgaatg attattgcat gtgtgtcggt
    15301 ttttgttgct taatcataag caacaggacg caggagtata aaaaatgaaa accgcttaca
    15361 ttgccaaaca acgccaaatt agcttcgtga aatctcactt ttctcgtcaa ctggaagaac
    15421 gtctggggct gatcgaagtc caggcaccga ttcttagccg tgtgggggat ggcacgcagg
    15481 ataacttgtc gggctgtgaa aaagcggtgc aggtaaaagt gaaagctctg cctgatgccc
    15541 agttcgaagt gattcattca ctggcgaagt ggaaacgtca gaccttaggg caacacgact
    15601 tcagcgcggg cgaagggctg tacacgcaca tgaaagccct tcgccccgat gaagaccgtc
    15661 tttctccgtt gcactcggtc tatgtagacc agtgggactg ggaacgcgta atgggcgacg
    15721 gtgagcgtca attctcgact ctgaaaagca cggtagaggc gatctgggcg ggaattaaag
    15781 caaccgaagc tgcggttagc gaagagtttg gcctggcacc gttcctgccg gatcagatcc
    15841 acttcgtaca cagccaggag ttactgtctc gttatctgga tctcgatgcc aaagggcgtg
    15901 agcgggcgat agcgaaagat cttggcgcgg tattccttgt cgggattggc ggcaagctga
    15961 gcgatggtca tcgccacgac gtgcgcgcac cggattatga tgactggagc accccgtcag
    16021 agctgggtca tgcgggtctg aacggcgata ttctggtgtg gaaccctgta ctggaagatg
    16081 cgtttgagct ttcttccatg gggatccgcg tggatgccga cacgctgaag catcagctgg
    16141 cgctgaccgg tgatgaagat cgtctgcagc tggaatggca tcaggcgctg ctgcgcggtg
    16201 aaatgccgca gaccatcggc ggcggtatcg gccagtctcg tttgactatg ctgctgctgc
    16261 aactgccgca tatcggccag gttcagtgtg gagtatggcc agctgctgtt cgcgagagcg
    16321 tcccttctct gctgtaataa tttatcgccg ccagcgtctg agcaggcggc ttcgcatccc
    16381 ggtatcaaag cgccagatat gatcgaaaat gcgcatgatg ccgggtttgc cgtgtgccga
    16441 catcgccacg gcatgaaagc gatgctgatg tacccgctgc agctctttca ctttactcgt
    16501 cacgtcgtca ggcaaccgct gagcgataaa atcagaaatc accaccgcat cggcatcaaa
    16561 ccattccctg ctttgcaagc gttccataat ggcgcgaaaa caactggcaa gatcggtgcc
    16621 gccacgaaac tgctggctta aaaaacggat tgcttgttcg atgccttgtg ggcctgaaag
    16681 ctcataacgg acgatctcgg tggaaaatag cataatatag cagcgccggt tttctgcgag
    16741 agcaatgcgc atcaaggcca ggcagaacgc tttcgcacac tgttcattaa agccgcccat
    16801 tgagccggaa gtatccacac agacaataaa cggcccgcgc ggctgttcat cgtaatcttt
    16861 atgtaccacc ggacgttcga tcactttttc acgccacgac tcaccgtgca ggcgataggt
    16921 gagcaactgt ttttccacca gccgacggta aaactcatac tccagttccg ttatccctag
    16981 tgtcgccagt tctggcggca ggagacgtaa aatatcatcg ctttgttgca gaccatcaac
    17041 ctgctcagga accgtcgccg gttcgcgcac catggtgcgg aaggtttcca tctgcgcatc
    17101 gttgcgcggt attgatttgg cttcccgaga acgccccagc tgctctgcca ggcgtttcag
    17161 ttccggctgt tcgttaagaa attcaccgta tttcacaatc aactgatagt cgccacgttt
    17221 aagctggccg gcgctcatat cccacagacg accagctgcg gtattgttat ctgcgagaat
    17281 cggttcaagt tgtccgctca gcgtcatgcg ttcctgaact tcacttaaca gttgttcgcg
    17341 ttcttcttct aatagctgtt gatttaacgt cgttgcttgc acgatcagac ttaaacgcca
    17401 gcgctggaga aaaagcgtgt gtaacgctga agttattgtg ctgttagcat caaccaactg
    17461 ccgggcttgt tctgcccatg gggaattcag acgatgcagt aagtccagga tctgtggtag
    17521 ctgcacaata aactgcggcg tggagaggag ctggctttgc tgatagcaca tcacctcttc
    17581 ggtgagttct ggcgggactc gggcatcttt cagccgactg cgcagcgcct cactccagcg
    17641 gggaacatca tcagtgattg ctgccttcag tcgtgggaat ttttcaaaga agaccgccag
    17701 ctgcggtgag gccagcagtg cgatgatcat ctcttcgatc aatccctctt cgctgacggc
    17761 cagcatcaca ttaagcgtat ccagcgttag cattgttgtg cctggcgaat ctgtgcaccg
    17821 acatcctgta ggctggcttc aatgcgacct aaccagtcgc cagggataaa cagacatttt
    17881 tgctgttcgc tgaataaagc gtgttgctta cgccagtcgc tttcaagctc ttccagttgt
    17941 tgttttattt cacctggcag accttcagcc gatgaaccgg gaagtgccag cgtactgcct
    18001 tgtaaactta catcgcgtac aacaagatgt tgggtgctat caacttccag attcagtttc
    18061 tgggcaaagc cgataccgtt cagtttgccg cgaatttcac cacctttact cagccactgt
    18121 tccagcgcgc tacgctcaaa ggagatatga accacttcca tatcatgcag ttttaacggt
    18181 ttttgcagca gcagagtcag agtggaagca gtaacgttaa cagggagttg atactgctga
    18241 cgacggctaa aaatgccgcc cagacgaatt accgttaagg ctgttttatc gctttgttgc
    18301 tgctgaagtt gcaggtgacg ttgcacaatc gcgcctaggc gggtcaacat cccttgctgt
    18361 tgccaggcgt gaccggtcat caatacatca atttgttgtt gtatcaaatt caggctttgc
    18421 gcgtcatacc acaggcaatc tttcagcaaa atgagatcta ccggggcaac agcactgcga
    18481 ccgctaaaaa aggcgctggc ctgcaataat cggatcgctt ttttccagcg acgatccgag
    18541 acataaggcg catccggtaa tttatccagt tgctggcgca gcataaaaat cagctcaaat
    18601 acatgatcgg gcagcgtaat ttcaccaatc tctttctgcc agcgttcata ttcttcatct
    18661 gtgacctgca aggcatcagg aaccggattg tcgttttcat cctgttgact ggtcagcatg
    18721 gagcggaaat tcgctttatc ctgcacttta tctaaccaca gacgaatcag catgcggtca
    18781 tataacgctt ccagactgct gtctgcttcc ggcagctcgt tggaggccgc caccagcaga
    18841 cgcatcggga ttttttctac gtgtgcaccg ttgcggaact ggcgctcgtt aatggcggtg
    18901 agcaaggtat taagaattgc cgggcccgct ttccagatct catccagaaa gacaatttct
    18961 gcttccggca ggtaaccgct ggttaaacgt tcatagcgcc cttcatcttt tagcgcctga
    19021 atagaaaggg gaccaaaaac ttcttccggc gtggagaagc gggtcatcag atattcaaac
    19081 gcgcgggcat tctgaaaggc gaattttaag cgccgggcga tcaaactttt ggcaatacct
    19141 ggcgggccaa ggaggaacac actttcacca cttaatgccg ctaataaaca caagcggatg
    19201 gcgtggctac gttcataaag ccccttttcc aacgaactgc tcaggcggga aattctttcc
    19261 gctaataaat gagggtgagc cataatgaag tggcgtcctt tcgtcaaaag ttctgcgtaa
    19321 attgcgagta tagacgtttc ttgctggtgg ctaaaatagt ctcaaagggg ggtaatgcca
    19381 gtcagttaag caactgactg g
//
LOCUS       DAJVUJ010000074        19313 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.074, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000074 DAJVUJ010000000
VERSION     DAJVUJ010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19313
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.074"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..732
                     /gene="rihB"
                     /locus_tag="OQQ48_002311"
     CDS             <1..732
                     /gene="rihB"
                     /locus_tag="OQQ48_002311"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HCS1953065.1"
                     /translation="IMRKQIVADNIHGETGLDGPVFESLTRQAESTHAVKYIIDTLMA
                     SDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFTPSAEFNIFADPE
                     AARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSDIMNFTLKTQFEN
                     YGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCDELGVLGKPANTK
                     VGITIDTDWFWGLVEECVRGYIKTH"
     gene            832..2082
                     /locus_tag="OQQ48_002312"
     CDS             832..2082
                     /locus_tag="OQQ48_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCS1953066.1"
                     /translation="MDVMRSVLGMVVLLTIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPAGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDHLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFAHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTGGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRVPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(2138..3226)
                     /locus_tag="OQQ48_002313"
     CDS             complement(2138..3226)
                     /locus_tag="OQQ48_002313"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCS1953067.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKGKGYILTEQEYCVVVGTINMDIRGIADIRYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTAPAHTTVDSFGAGDGFMAGLVYSFLEGYSFRDSARFAVA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(3229..4086)
                     /gene="nfo"
                     /locus_tag="OQQ48_002314"
     CDS             complement(3229..4086)
                     /gene="nfo"
                     /locus_tag="OQQ48_002314"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCS1953068.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(4160..5209)
                     /locus_tag="OQQ48_002315"
     CDS             complement(4160..5209)
                     /locus_tag="OQQ48_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HCS1953069.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            5309..6190
                     /locus_tag="OQQ48_002316"
     CDS             5309..6190
                     /locus_tag="OQQ48_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1953070.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANIPR"
     gene            6395..7864
                     /gene="lysP"
                     /locus_tag="OQQ48_002317"
     CDS             6395..7864
                     /gene="lysP"
                     /locus_tag="OQQ48_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HCS1953071.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            8157..10137
                     /gene="cirA"
                     /locus_tag="OQQ48_002318"
                     /pseudo
     CDS             8157..10137
                     /gene="cirA"
                     /locus_tag="OQQ48_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
     gene            complement(10169..11005)
                     /gene="yeiG"
                     /locus_tag="OQQ48_002319"
     CDS             complement(10169..11005)
                     /gene="yeiG"
                     /locus_tag="OQQ48_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HCS1953072.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSSYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            11263..11931
                     /gene="folE"
                     /locus_tag="OQQ48_002320"
     CDS             11263..11931
                     /gene="folE"
                     /locus_tag="OQQ48_002320"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HCS1953073.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            11948..13105
                     /locus_tag="OQQ48_002321"
     CDS             11948..13105
                     /locus_tag="OQQ48_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HCS1953074.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNRAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTMLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGTAISKTSR"
     gene            13247..14287
                     /gene="galS"
                     /locus_tag="OQQ48_002322"
     CDS             13247..14287
                     /gene="galS"
                     /locus_tag="OQQ48_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HCS1953075.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAIFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            14567..15565
                     /gene="mglB"
                     /locus_tag="OQQ48_002323"
     CDS             14567..15565
                     /gene="mglB"
                     /locus_tag="OQQ48_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HCS1953076.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQSVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            15626..17146
                     /gene="mglA"
                     /locus_tag="OQQ48_002324"
     CDS             15626..17146
                     /gene="mglA"
                     /locus_tag="OQQ48_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HCS1953077.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLRRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEELRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDKSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            17162..18172
                     /gene="mglC"
                     /locus_tag="OQQ48_002325"
     CDS             17162..18172
                     /gene="mglC"
                     /locus_tag="OQQ48_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HCS1953078.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPCWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(18415..19313)
                     /gene="preA"
                     /locus_tag="OQQ48_002326"
                     /pseudo
     CDS             complement(18415..19313)
                     /gene="preA"
                     /locus_tag="OQQ48_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
BASE COUNT         4601 a         4774 c         5120 g         4818 t
ORIGIN      
        1 attatgcgta aacaaatcgt tgccgataat attcacggtg aaaccggact ggatggtccg
       61 gtattcgagt cgctgacccg ccaggcagaa agtacccacg cggtgaaata catcattgat
      121 accctgatgg caagcgatgg cgatatcact ctggtgccgg ttggtccgct ttcaaatatc
      181 gcggtggcaa tgcgtatgca acccgcgatc ctgcccaaaa tccgtgaaat tgtgctaatg
      241 ggcggcgctt acggtacagg caacttcacg ccatctgccg agttcaacat ctttgctgat
      301 ccggaagcag cccgcgtggt gttcacctcc ggcgttccat tagtgatgat gggcctcgat
      361 ctcaccaacc agactgtttg caccccggac gtgattgcac ggatggaaag ggcaggcggc
      421 cctgccggag agctgttcag cgacatcatg aacttcactc tcaaaacgca gttcgaaaac
      481 tacggcctgg ctggcggccc ggtgcacgac gccacctgca tcggttatct gattaaccct
      541 gatggcatta aaacccagga gatgtacgtc gaagtggacg tcaacagtgg cccttgctat
      601 gggcgtaccg tctgcgacga gctgggcgtt cttggcaagc ccgctaatac caaagtcggc
      661 atcactattg ataccgactg gttctggggc ttagtcgaag agtgcgtgcg cggctacatc
      721 aaaacccatt aagtgttgat aacaagccgg gtcagtatcg caagatccaa gcccggctat
      781 ttctgatggg cagaaagccg tcgcccaata tgacatgaag agataataac tatggatgtc
      841 atgagaagtg ttctgggaat ggtggtattg ctgacgattg cgtttttgct gtcagtaaac
      901 aagaagaaga tcagcctgcg taccgttggc gcggcgttag tgttacaggt cgtgattggc
      961 gggattatgc tttggttacc ggcagggcgt tgggtcgctg aaaaagtcgc ttttggcgtg
     1021 cataaagtga tggcgtacag cgacgcgggt agcgcattta tcttcggttc gctggtcggg
     1081 ccgaaaatgg acacgctgtt tgatggcgca ggatttatct ttggtttcag ggtattaccg
     1141 gcaattatct tcgtcactgc actggtgagt attctctact acatcggtgt gatggggatt
     1201 ttaattcgca ttctcggcgg tatattccag aaagcattaa atatcagcaa gattgagtca
     1261 ttcgtcgcgg tcaccaccat tttcctcggg caaaacgaaa ttccggcgat cgtcaaaccc
     1321 tttatcgatc atctgaatcg caatgaatta tttacagcga tttgtagtgg catggcctcg
     1381 attgctggtt cgacaatgat tggttacgcc gccctgggcg tacctgtgga atatttgctg
     1441 gcggcatcgt taatggcgat cccaggcggg atcttgtttg ctcgcctgtt aagcccggct
     1501 acggaatctt cgcaggtttc ctttaataac ctctctttca ccgaaacacc gccaaaaagc
     1561 attattgaag ccgctgcgac aggggcaatg accgggctga aaatcgccgc cggtgtagcg
     1621 acagttgtta tggcatttgt cgccatcatt gcgttaatta atggtattat cggcggcgtt
     1681 ggcggctggt ttggttttgc acatgcctcg ctggagtcca ttttaggtta cctgttggcc
     1741 ccattggcgt gggtgatggg ggttgactgg agtgatgcaa atcttgccgg gagtttgatt
     1801 gggcagaagc tggcgatcaa tgaatttgtc gcttatctca atttctcgcc atatctgcaa
     1861 acgggtggca ctctggatgc taaaaccgtg gcgattattt ctttcgcgtt gtgcggtttc
     1921 gctaactttg gttctatcgg ggtggtggtg ggggcgtttt ctgcggttgc gccacaccgt
     1981 gtgccggaaa tcgcccaact tggtttacgc gcgctggcgg cggcgacact ttctaacctg
     2041 atgagtgcta ctattgcagg attctttatt ggtttagcgt agtgcgcaat ttatgagccg
     2101 gataagatgc ggtcaagcgt cgaatccggc aacattgtta caccattggc actaatgaaa
     2161 gcgcgttatc ggcagacagg gtagggttgt ttaagctgcc gctggcgcgc gaaattgccg
     2221 cgcaggctac cgcaaaacgg gcgctgtcgc ggaaactgta tccttcaaga aagctatata
     2281 ccaggcccgc cataaaacca tcgccagcac caaaactgtc tactgtcgta tgcgctggcg
     2341 cggtcagcaa aaattgttct ccatcctttt cgctgcaata aactgactca tcgggcaaat
     2401 aaacaaacag ttgctgaaca ccttgctgat gcaatgcatt cactgcggca ttacggtcag
     2461 catcgctggt gatcgcctgt ccccataaaa tttccagctc cggtaaagtg ggtttcaggg
     2521 tgtgaatatg cgccagccag tgtttgattt tgcccgcttt gaattctgaa acggtatcga
     2581 caaacaccgg gatttcatca gcgagggtaa agacccattc cagcgcctcg gctgtcaggt
     2641 tgcaatcagc cagtaccacg cccgcatgac gaagtaaatc gcgcgacccg ttcagtagtt
     2701 gcggtgtcaa ctgttccagc agatgggtat cgttaatcgc cagcacggtt tgatcgtctc
     2761 gattggcaat tgccagatac gtcgatgtgc tttgaccatg caaacgaacg cagccggaga
     2821 cattcacgcc ggcacggcgc gtttcttcca ggagcatttc gccataaaag tcatcgccaa
     2881 tcactgaaag caaatggacg tcacggccta acagcgccag attgtgggcg atgttgcgtc
     2941 ccacgccgcc cgccgagcaa tgaattgtac cgggatgaga agccgcttgc gggtaacgga
     3001 tatccgcgat cccgcgaata tccatattga ttgtccccac cactacgcag tattcctgct
     3061 cggtgagaat gtaacctttg cctttaatcc gccctttgcg cattaaatcc ataatatgcg
     3121 ccgcaacgcg cgaacgactg atttgcagca tgtccgcaat ttcgttctgc tgaatcagcg
     3181 ggttacgccg taaaattgca aggatctcct tttcccggtt attcatcttc aggctaccgc
     3241 tttttcagtt tgttgcgctt tcagccaggc gatctcttct gcccagatat ccgggttgat
     3301 ggtttcaagg atcagcggaa tgccgtcgaa acggtcgtcc tgcatgatcc agcggaacgc
     3361 atcatgaccg atattgcctt caccgaggct atgatggcgg tcaacgcggc tgccaaaggt
     3421 gcttttcgca tcgttaaggt gcatcccgcg cagatactta aagccaacaa tacgggcaaa
     3481 atccgcgaac gttttctcgc attcggctgg agtacgcaaa tcatacccgg cagcgaaagc
     3541 atggcaggta tcaatgcaga cgccgacacg ggatttatct tccacgccgt cgataatcgc
     3601 cgcgagatgt tcgaatttaa accctaagtt actgccctga ccggcggtgt tttcaatcac
     3661 cgctgttaca ccttgagttt tatccagcgc aatgttgatg gattcggcaa tacgcgcaag
     3721 gcaatcctct tctgaaatct gcatcagatg gctgccaggg tggaagttga gcaaagaaag
     3781 ccccagttgt tcgcaacgct gcatttcatc tataaaggca tcgcgcgatt tttccagagc
     3841 ttcagtgacc ggatgtccga ggttaatcag atagctgtcg tggggaagaa tttgcgccga
     3901 tgtgtagtga tatttttcac aggctgcttt gaattcatcg atggtttgcg tcgtgagcgg
     3961 tgcggcacgc cactgacgtt ggtttttggt gaacaaggca aacgcggttg cgtcgatttc
     4021 ggcggcgcga attgcggcat ttgccagacc gccagcagca ctaacgtgcg ctccaatgta
     4081 tttcatgcga ggactcctgt taaacccgct ggaggaaaac ggtaatgata gcgggttaac
     4141 aggagcaaga tgtagtggtt tatgcgatga cgctttgaat cacatagtta atcgcaccac
     4201 caccaacaat cagccaggca aacagtacca gtgccatcag cagaggtttc gccccggctt
     4261 ttttcagcgc gctgacgtga gtggtcagac ccagcgccgc catcgccatt gccagcagga
     4321 aggtatccag cgttaccagc atgttcacca cgctctgcgg taacaggtgg aacgagttaa
     4381 agatggcaac gacgatgaac aagatggcaa accacggaat agtgattttg cttttctcgc
     4441 cgctgttcgc cccagacagc tgtttaacac gcgccgccag caggatgagg aacggagcca
     4501 gcatcatcac gcgcagcatt ttggaaataa ctgctgcgtt ttccgcatcc gggctgatgg
     4561 catgacctgc cgccaccacc tgcgccactt cgtgcacagt agaaccaatg tagataccga
     4621 aagtttccgg actaaaccat tgagacatca gcggatatat cgccgggtag aggaaaatcg
     4681 cgacggtccc gaagataaca acggttgcaa cagccacggt tactttactg gcttccgctt
     4741 tcactaccgg ctcagtcgcc agtaccgcgg cagcaccaca gatactgcta ccggcaccga
     4801 tcaaccagct ggtgtgctta tccagaccaa acactttctg ccccaggaag caagccagca
     4861 ggaaggtact ggacagcgtc aacacgtcaa tgatgatccc actgataccg acatcggcaa
     4921 tttgcgagaa cgtcagacgg aagccataaa gaatgatacc cagacgtaat aaatattgct
     4981 tggcaaacag cacaccaccg tcacagcttt tccagatgtg cggatagatg gtgttgccta
     5041 aaaccatccc caacaagatt gcgagggtga gggcactaaa cccggcaccc gcaaccgcgg
     5101 gaatggaacc accccacagg gcgaccccgg tgataactgc actcagggct aaccccggaa
     5161 taaaatgcca cagtgtacga tgttgtttct gtaaggtgat attcgtcata accctctcct
     5221 tataaccggg cataaggtta cgactaactg gtttaaaaat aaaattgatt atatatttat
     5281 aattaatctt tataagtggt aagcgactat gcacatcacc ctccggcagt tggaagtttt
     5341 tgcagaagta ttgaaaagtg gatcaaccac ccaggcgtcg gtgatgctgg cgttgtcgca
     5401 atcagcagtg agcgcagcct tgaccgacct ggaagggcag cttggcgtgc aactgtttga
     5461 tcgcgtgggg aaaagactgg ttgttaatga acacgggcgg ctgctctatc cgcgtgcgtt
     5521 ggcattgctt gaacaggcgg ttgaaatcga acaactgttt cgcgaagaca acggcgcgat
     5581 tcgtatctat gccagtagta ccatcggtaa ctacattctg cctgcagtta tcgcccgtta
     5641 tcgccatgat tatccgcagt tgccgattga acttagcgtt gggaacagcc aggacgtgat
     5701 gcaagcggtg ctggatttcc gcgttgatat tggctttatt gaaggaccgt gccacagcac
     5761 tgaaatcatt tctgaaccgt ggctggaaga cgagctggtg gttttcgccg cgccgacttc
     5821 gccgttggct cgtggtccgg tcaccttaga acagctggcc gctgcgccgt ggatcctgcg
     5881 tgaacgcggt tccggcacgc gggagattgt cgattatcta ttgctgtcac atttaccgaa
     5941 gtttgagatg gcgatggaat taggtaactc cgaggcaatc aaacatgcgg tgcgtcatgg
     6001 gttgggaatt agttgcctgt cgcgacgggt gattgaagat caattgcagg caggcacatt
     6061 aagtgaagtt gcggtccctc tgccgcgcct gatgcgtacg ttgtggcgta tacatcatcg
     6121 gcaaaaacac ctttccaacg cgctacggcg ctttctggac tattgcgatc ccgcgaatat
     6181 accgcgttaa gttgctgtac aagaacatgc tggtgctgtg tcgatttcgt gacgcagcgc
     6241 cctcagcatg cattcgccag aaaagagatt ggctgcttta cttataatcc cagggcgatc
     6301 atgaaggtgt cttataaccg tgtatttctg ccggaaggat tgccaatcgt ctgctacaat
     6361 cgcgcctcat ttttaagatg gatagcattt ttgtatggtt tccgaaacta aaaccacaga
     6421 agcgccgggc ttacgccgtg aattaaaggc gcgtcacctg acgatgattg ccattggcgg
     6481 ttccatcggt acaggtcttt ttgttgcctc tggcgcaacg atttctcagg caggtccggg
     6541 cggggcattg ctctcgtata tgctgattgg cctgatggtt tacttcctga tgaccagtct
     6601 cggtgaactg gctgcatata tgccggtttc cggttcgttt gccacttacg gtcagaacta
     6661 tgttgaagaa ggctttggct tcgcgctggg ctggaactac tggtacaact gggcggtgac
     6721 tatcgccgtt gacctggttg cagctcagct ggtcatgagc tggtggttcc cggatacacc
     6781 gggctggatc tggagtgcgt tgttcctcgg cgttatcttc ctgctgaact acatctcagt
     6841 tcgtggcttt ggtgaagcgg aatactggtt ctcactgatc aaagtcacga cagttattgt
     6901 ctttatcatc gttggcgtgc tgatgattat cggtatcttc aaaggcgcgc agcctgcggg
     6961 ctggagcaac tggacaatcg gcgaagcgcc gtttgctggt ggttttgcgg cgatgatcgg
     7021 cgtagctatg attgtcggct tctctttcca gggaaccgag ctgatcggta ttgctgcagg
     7081 cgagtccgaa gatccggcga aaaacattcc acgcgcggta cgtcaggtgt tctggcgaat
     7141 cctgttgttc tatgtgttcg cgatcctgat tatcagcctg attattccgt acaccgatcc
     7201 gagcctgctg cgtaacgatg ttaaagacat cagcgttagt ccgttcaccc tggtgttcca
     7261 gcacgcgggt ctgctctctg cggcggcggt gatgaacgca gttattctga cggcggtgct
     7321 gtcagccggt aactccggta tgtatgcgtc tactcgtatg ctgtacaccc tggcgtgtga
     7381 cggtaaagcg ccgcgcattt tcgctaaact gtcgcgtggt ggcgtgccgc gtaatgcgct
     7441 gtatgcgacg acggtgattg ccggtctgtg cttcctgacc tccatgtttg gcaaccagac
     7501 ggtatacctg tggctgctga acacctccgg gatgacgggt tttatcgcct ggctggggat
     7561 tgccattagc cactatcgct tccgtcgcgg ttacgtattg cagggacacg acattaacga
     7621 tctgccgtac cgttcaggtt tcttcccact ggggccgatc ttcgcattca ttctgtgtct
     7681 gattatcact ttgggccaga actacgaagc gttcctgaaa gataccattg actggggcgg
     7741 cgtagcggca acgtatattg gtatcccgct gttcctgatt atttggttcg gctacaagct
     7801 gattaaagga actcacttcg tacgctacag cgaaatgaag ttcccgcaga acgataagaa
     7861 ataagtttcc tcccttcctt gctaagccct ctcaaccgag agggcttttt caattccatt
     7921 tccctgacaa atcatgcgga tataaaattt aacatttgga ttgataattg ttatcgtttg
     7981 cattatcgtt acgccgcaat caaaaaaggc tgacaaatca gaggctgttc cggctttctg
     8041 ggatgatcac ctgcatacaa ataagtccac cgcgatgctg ccgtacgcaa ggggacgtga
     8101 agaaggtgtg agcgataacc cattttattt tcgtagttac ctcatggaga tatggaatgt
     8161 ttaggttgaa ccctttcgta cgggtcaggc tgtgtttgtc cgctatttct tgtgcatggc
     8221 ctgtgttagc ggtcgatgat gatggcgaaa cgatggttgt cactgcatct tccgttgaac
     8281 aaaacctcaa agatgcaccc gccagtatca gcgtcattac ccaggaagac ctgcagcgaa
     8341 aaccggtaca gaatctgaag gatgtcctca aagaagtgcc tggcgtacaa ctgacgaacg
     8401 aaggggataa ccgtaagggc gttagtattc gtggtctgga cagcagctac accctgattc
     8461 tcgtcgacgg taaacgcgtt aactcccgca atgccgtctt ccgccacaat gatttcgatc
     8521 tgaactggat cccggtcgat tccatcgaac gtattgaagt ggttcgtggc ccgatgtcgt
     8581 cgctatacgg ctccgatgcg ctcggcggtg tagtgaatat catcaccaaa aaaatcggtc
     8641 agaaatggtc gggtaccgtt accgtcgata ccaccattca ggaacatcgc gatcgcggtg
     8701 atacctataa cggtcagttc tttaccagtg gaccattaat tgatggtgtg ctgggaatga
     8761 aagcttacgg cagcctggca aaacgtgaaa aggatgaccc gcaaaactca acgaccaccg
     8821 ataccggaga aatgccgcgt attgaaggat tctccagccg cgacggcaat gtcgaatttg
     8881 cctggacacc gaatcaaaat cacgatttta ctgccggata cggtttcgac cgtcaggaac
     8941 tcgctggaca aaaaccgcct ggaacgccag aactactccg tcagccataa tgggcgttgg
     9001 gattacggca ccagcgaact gaaatactac ggtgagaaag tcgagaacaa aaaccctggc
     9061 aacagcagcc cgataacttc cgaaagcaat acggtcgacg gcaaatacac gttgccgctg
     9121 acggcgatta atcagtttct cacggttggc ggtgaatggc gtcacgacaa acttagcgat
     9181 gcggtgaacc tgaccggggg aaccagctcc aaaacgtctg ccagccagta cgcgctgttt
     9241 gtggaagatg aatggcggat cttcgagccg ctggcgctga cgaccggcgt gcgtatggac
     9301 gatcacgaaa cctacggtga acactggagt ccgcgtgcct acctggttta taacgccacc
     9361 gacaccgtaa cggtgaaagg gggctgggcg acggcattta aagcaccttc tctgttgcaa
     9421 cttagccctg actggacgag caattcctgc cgtggcgcat gtaagattgt gggtagcccg
     9481 gatctgaaac cagaaaccag cgaaagttgg gagctggggc tttactacat gggtgaagaa
     9541 ggctggctgg aagggggtga atccagcgtt accgttttcc gtaacgatgt gaaagatcgt
     9601 atcagcatca gccgtacgtc tgacgtcaac gctgcaccgg gctaccaaaa ctttgttggt
     9661 tttgagacgg gcgctaacgg acggcgcata ccggtattta gctactacaa cgttaacaaa
     9721 gctcgtattc agggcgtgga aaccgaactg aaaattccgt tcaacgatga atggaaactg
     9781 tcgatcaact acacctacaa cgatggtcgt gatgtcagca acggcgaaaa caaaccgcta
     9841 tccgatctgc cgttccatac tgctaacggt acgctggact ggaaaccgct ggcgctggaa
     9901 gactggtcat tctatgtttc tggtcactat accgggcaga aacgcgccga cagcgcgacg
     9961 gctaaaacac cgggcggtta taccatctgg aataccggcg cggcctggca ggtgactaaa
    10021 gacgtcaaac tgcgcgcagg cgtgctgaac cttggcgaca aggatctcag tcgtgacgac
    10081 tacagctata acgaagacgg acgtcgttac tttatggcag tggattatcg cttctgatga
    10141 gaagatgccc ggcgaaccgg gcggactttc acttcagtaa atactgcgca tggaagcgca
    10201 ggtgatcctc tataaaagag gcgatgaagt agtaactgtg atcatatccc ggctgaatac
    10261 gcagcgtcat cggccacgct ttctggcgcg cggcttctgc cagtaccgca ggttgcaact
    10321 ggtcggcaag aaactgatca ttatcgccct gatcgataag cgtcgggatc gcatcctgcg
    10381 cgttactggc atacatcagc gcgcaactgt cccattccag ccatgcattt ttgtcctcac
    10441 ctaaatagct gctaaacgct ttgattcccc acgggacgct gcacggattc acaattggcg
    10501 caaaggccga aacgctggtg tatttacccg gatttttcag cgccataatc agcgcaccgt
    10561 gaccacccat tgagtgaccg ctaatggcgc agcggtcgct gacattaaat tgcgactgaa
    10621 ccagcgccgg taattcatcg cgcagataat catacatccg gtaatgcgtc gcccacggcg
    10681 gttgcgtggc attaagataa aagcctgcgc cctggcctaa atcgtagcca tcgtcgttgg
    10741 caaccttttc gccgcgcggg ctggtgtctg gcatcaccag tacaatcccc agttctgccg
    10801 ccacgcgctg ggcacccgcc ttggtggtga agttctcgtc attgcaggtt aatccggaaa
    10861 gccagtacag cactggtggc ggggtgtgat cacgaggggg tgggagaaag atactgaacg
    10921 tcatcgggca gtttaaggta ctggagtcgt gtcgccagcg ttgctgccag ccttcaaaac
    10981 agcggtgctc ttcgagcatt tccatgcgtg gctccttgtt gtgttgtttg caatttcttc
    11041 atcataccgt ttattcaatg tgctgtgagt aactttcact tccgtatttg cataacgatg
    11101 ttttaacatc tgctgatgaa aggcagcggc aattacaata attatcgctg tgaatactgg
    11161 attatgtgcg ccgcctcacg cacaataatc aggctgtaaa tcagcttaat aactttgccc
    11221 ccacgcaggg cggaggcgtc acacctgcag gagaaatcat aaatgccatc actcagtaaa
    11281 gaagcggccc tggttcatga agcgttagtt gcgcgaggac tggaaacacc gctgcgcccg
    11341 cccgtgcatg aaatggataa cgaaacgcgc aaaagcctta ttgctggtca tatgaccgaa
    11401 atcatgcagc tgctgaatct cgacctggct gatgacagtt tgatggaaac gccgcatcgc
    11461 atcgctaaaa tgtatgtcga tgaaattttc tccggtctgg attacgccaa cttcccgaaa
    11521 atcaccctca ttgaaaacaa aatgaaggtc gatgaaatgg tcaccgtgcg cgatatcact
    11581 ctgaccagta cctgtgaaca ccattttgtt accatcgatg gcaaagcgac ggtggcttat
    11641 atcccgaaag attcggtgat cggtctgtca aaaattaacc gtatcgtgca gttctttgcc
    11701 cagcgtccgc aggtgcagga acgtctgacg cagcaaattc ttattgcgct acaaacgctg
    11761 ctgggcacca ataacgtggc tgtctccatt gatgcggtgc attactgcgt gaaagcgcgt
    11821 ggcatccgcg atgcaaccag tgccacaaca acgacctctc ttggtggatt gttcaaatcc
    11881 agtcagaata cgcgccacga gtttctgcgc gctgtgcgtc atcacaactg attaaaaggc
    11941 aggaaccatg gagcgcaacg taacgctcga ttttgttcgc ggcgtcgcca ttctggggat
    12001 cctgctatta aacatcagcg cctttgggct accaaaggcg gcttatctca atcgcgcctg
    12061 gtacggcgct attacgccgc aggatgcatg gacctgggca tttctcgatc tcatcggcca
    12121 ggtgaaattc ctcacgcttt ttgcgctgct gtttggtgcg ggcctgcaaa tgttgctgcc
    12181 ccgtggcaga cgctggatcc agtcgcggtt aacgctgtta gtcttgctgg gttttattca
    12241 cggtttattg ttctgggacg gcgatattct gctggcttac gggctggtgg gcttaatctg
    12301 ctggcggctg gtgcgcgatg cgccatcggt aaaaagcctg tttaatacag gcgtcatgct
    12361 ttatctggtg gggcttggcg ttttgctgtt attggggctg atttccgaca gccagaccag
    12421 ccgcgcctgg acgccggatg catcggctat tttgtatgaa aaatactgga agcttcacgg
    12481 cggcgttgaa gcgatcagta atcgtgccga tggtgttggc aacagtttac tggcactggg
    12541 cgcacagtat ggctggcaac tggcagggat gatgctcatt ggtgcggcgc tgatgcgcag
    12601 cggctggctg aaagggcagt tcagcttacg tcactatcgt cgtactggtt ttgtgctggt
    12661 ggcgattggg gtgaccatta accttcctgc catcgccctg caatggcagc tggactgggc
    12721 gtatcgctgg tgtgccttct tacttcaaat gccgcgggaa ctgagtgcgc cgtttcaggc
    12781 gattggctat gcgtcgctgt tttatggctt ctggccgcaa ttgagccgct ttaagctggt
    12841 gcttgcgatc gcctgcgtcg gacggatggc gctgaccaac tatctattgc aaacgctgat
    12901 ttgtaccatg cttttttacc acctcggttt gtttatgcat tttgaccgcc tggagctgct
    12961 ggcgtttgtt attccggtat ggctggcgaa tatcctcttc tctgttatct ggctgcgtta
    13021 cttccgccag gggccggtgg aatggctctg gcgtcagtta actttgcgtg ctgccggaac
    13081 ggcaatatct aaaacatcaa gataacgatc tggatcacat tcgttaacaa aacggctgta
    13141 accgtttcca ttgctgtgac tcgattcacg aagtcctgta ttcagtgctg acaaaatagc
    13201 cgccagcaag cagtcattta ctgcaatctc ataacaggta gtgaatatga tcaccattcg
    13261 tgatgtagcg cgtcaggctg gcgtctctgt ggcaacggtt tcccgggtgc tcaataacag
    13321 cacgctggtc agtgccgaca cgcgtgaagc agtaatgaaa gccgtgagtg agctggatta
    13381 tcggccaaac gccaatgccc aggcgctggc aactcaggtt agcgacacca ttggcgtggt
    13441 ggtgatggac gtttctgatg cgtttttcgg cgcgctggta aaagcggtgg atctagtcgc
    13501 tcagcagcat cagaaatacg tgctaatcgg caatagctat catgaagcgg aaaaagagcg
    13561 tcacgccatt gaggtgttaa ttcgccagcg ttgtaatgcg ttgattgttc actcaaaagc
    13621 attgagtgac gatgaactgg cgcaatttat ggataacatt cccggtatgg tgttaatcaa
    13681 ccgcgttgtg ccggggtacg cccatcgttg cgtttgcctg gataatctca gcggtgcccg
    13741 aatggcgacg cgcatgttgc tgaataacgg tcatcaacgt attggttatc tttcttccag
    13801 tcacggcatt gaagatgacg ccatgcgtaa agcaggctgg atgagtgcgt tgaaagagca
    13861 ggatattatt ccgccggaaa gctggattgg cactggtacg ccggacatgc cgggcggtga
    13921 ggcggcgatg gttgaactgc tggggcgcaa tctacaactt accgctatat ttgcttataa
    13981 cgacaatatg gccgctggcg cactgacagc attaaaagat aatggcattg cgattccgtt
    14041 acatctctca atcatcggtt tcgatgatat tcccatcgcc cgttacaccg acccgcaatt
    14101 aacgaccgtg cgttatccca ttgcttcaat ggctaaatta gccaccgaac tggccttgca
    14161 gggggcagca ggcaatattg atcctcgtgc cagccactgt tttatgccga cgttagtgcg
    14221 tcgccattct gtcgcaacgc gccagaatgc ggcggcgatc actaactcaa caaatcaggc
    14281 gatgtaaccg ctttcaatct gtgagtgatt tcacagtatc ttaacaatgt gatagctatg
    14341 attgcaccgt tttaacgttg taacccgtat gtaacagtga ataatcactt tcaccgaggt
    14401 aatagcgtca taacaacaat taaagccgtt ttctggagcg ttaccgggca tggaagaacg
    14461 aattttaaaa agtgagcttc ggcgttcagt aacacttcat taactctact gccctgccga
    14521 gcatttatct caagcactac cctgcataag aaaaaccgga gataccatga ataagaaggt
    14581 gttaaccctg tctgctgtga tggccagcat gttattcggt gccgctgcac acgctgctga
    14641 tactcgcatt ggtgtaacaa tctataagta cgacgataac tttatgtctg tagtgcgcaa
    14701 ggctattgag caagatgcga aagccgcgcc agatgttcag ctgctgatga atgattctca
    14761 gaatgaccag tccaagcaga acgatcagat cgacgtattg ctggcgaaag gggtgaaggc
    14821 actggcaatc aacctggttg acccggcagc tgcgggtacg gtgattgaga aagcgcgtgg
    14881 gcaaaacgtg ccggtggttt tcttcaacaa agaaccgtct cgtaaggcgc tggatagcta
    14941 cgacaaagcc tactacgttg gcactgactc caaagagtcc ggcattattc agggcgattt
    15001 gattgctaaa cactgggcgg cgaatcaggg ttgggatctg aacaaagacg gtcagattca
    15061 gtccgtactg ctgaaaggtg aaccgggcca tccggatgca gaagcacgta ccacttacgt
    15121 gattaaagaa ttgaacgata aaggcatcaa aactgaacag ttacagttag ataccgcaat
    15181 gtgggacacc gctcaggcga aagataagat ggacgcctgg ctgtctggcc cgaacgccaa
    15241 caaaatcgaa gtggttatcg ccaacaacga tgcgatggca atgggcgcgg tagaagcact
    15301 gaaagcacac aacaagtcca gcattccggt gtttggcgtc gatgcgctgc cagaagcgct
    15361 ggcgctggtg aaatccggtg cactggcggg cacagtactg aacgatgcta acaaccaggc
    15421 gaaagcgacc tttgatctgg cgaaaaacct ggccgatggc aaaggtgcgg ctgatggcac
    15481 caactggaaa atcgacaaca aagtggtccg cgtaccttat gttggcgtag ataaagacaa
    15541 cctggctgaa ttcagcaaga aataagactg attgggcgca gtcgtttact gcgcccttat
    15601 tgtatggcca acaaggtaaa attatatggt cagctcaacg actccgtctt ccggggaata
    15661 cttgttggaa atgagcggta tcaacaagtc ctttcctggt gttaaggcac ttgataacgt
    15721 taatttaaaa gtccggccac attctatcca tgcattaatg ggggaaaacg gtgcaggaaa
    15781 atcgacatta ttaaaatgcc tgtttggtat ttatcaaaaa gactccggca ccattttatt
    15841 ccagggtaaa gagatcgatt tccattccgc caaagaggca ctggaaaatg gtatttcgat
    15901 ggtacaccag gagttaaacc tggtattaca acgttcggtg atggataaca tgtggctgag
    15961 gcgatatccc accaaaggca tgtttgtcga tcaggacaaa atgtaccgcg aaaccaaagc
    16021 gatttttgat gaactggata ttgatatcga tccgcgtgcg cgcgtcggca cattatccgt
    16081 ttcgcaaatg cagatgatcg aaatcgccaa agcgttttcc tataacgcga aaattgtgat
    16141 tatggatgaa ccgacttctt cgttaaccga aaaagaggtc aatcatctgt tcactattat
    16201 tcgtaaatta aaagagcgcg gctgcggtat tgtttatatc tcgcataaaa tggaagaaat
    16261 cttccagtta tgtgatgaag ttaccgtatt gcgcgacggt cagtggatcg ccaccgaacc
    16321 gctggcagga ctgacgatgg acaagatcat cgccatgatg gttgggcgtt ctcttaatca
    16381 gcgttttcct gacaaagaaa acaagccggg cgaagtcatc ctcgaggtgc gtaacctaac
    16441 gtcactgcgc cagccgtcga ttcgcgatgt ctcgtttgat ctgcataaag gggagatcct
    16501 cggtattgcc gggctggtgg gggcgaaacg taccgatatt gttgagacgt tatttggtat
    16561 tcgcgagaaa tcggctggca ccattacgtt gcacggcaaa cagatcaata accataatgc
    16621 caacgaagcc ataaaccacg gatttgcact ggtaactgag gagctccgct caacgggaat
    16681 ttatgcctat ctggatattg gttttaactc gttaatttcc aatattcgca actacaaaaa
    16741 taaagttggt ttactggata aatcgcggat gaaaagcgat acccagtggg tgattgattc
    16801 gatgcgggta aaaacaccgg ggcatcggac gcaaattggt tcgctctcag gtggtaatca
    16861 gcaaaaggtg attattgggc gctggctact aacgcaacca gaaatattaa tgctcgatga
    16921 accgacgcgc ggtattgatg tcggggcgaa gtttgagatc tatcagttaa ttgccgaact
    16981 ggcgaagaaa ggcaaaggga ttattattat ctcctctgaa atgcctgagt tgttagggat
    17041 aacagaccgt attctggtca tgagcaatgg tctcgtttcc ggaattgtcg acacaaaaac
    17101 aacaacgcaa aacgaaattc tgcgtcttgc gtctttgcac ctttaagatt aggggcttcc
    17161 catgagtgcg ttaaataaga aaagttttct tacttacctg aaagagggcg gtatttacgt
    17221 cgttctttta gttttgctgg cgattattat tttccaggac ccaacatttt taagtctgtt
    17281 gaacttaagt aatattctca cccagtcatc ggtgcgtatt attatcgcgc tcggtgtggc
    17341 agggttaatt gtcacccagg ggaccgacct ttctgcgggg cgtcaagtag ggctggcagc
    17401 agtggtggca gcgacattat tgcagtccat ggataacgcc aataaagtgt tcccggaaat
    17461 ggcgacgatg ccgattgcgc tggttattct gattgtctgt gccattggtg cggtgatcgg
    17521 tttgatcaac ggtctgatta tcgcttatct caacgttacg ccgttcatta ccacgctcgg
    17581 cacgatgatc atcgtctatg gcatcaactc gctctattac gactttgtcg gggcgtcgcc
    17641 aatttctggt tttgacagtg gcttctctac ctttgctcag ggctttgtcg cgctggggag
    17701 tttccgtctc tcttacatca ccttctacgc gttgattgcg gtggcgttcg tctgggtgtt
    17761 gtggaacaaa acccgcttcg gtaagaacat ttttgccatt ggcggtaacc cggaagcggc
    17821 aaaagtatct ggtgtcaacg tcggcctgaa cctgctgatg atctacgcgt tgtctggcgt
    17881 gttctatgcc tttggcggga tgttagaagc cggacgtatc ggctctgcca ccaacaacct
    17941 cggctttatg tatgagctgg atgctatcgc ggcgtgcgtg gtaggcggtg tatcgttcag
    18001 cggcggtgtg gggacggtga ttggcgtggt gaccggggta attattttta ccgtcatcaa
    18061 ctatggcctg acgtatatcg gcgtaaaccc atgctggcag tacatcatca aaggggcgat
    18121 tattatcttc gccgtagcgc tggattcact gaaatacgcg cgtaagaaat gatgtgaatg
    18181 tggtccgcat tcggtcataa acgcacataa catcatgatc tgtgtggttt tgtaggccgg
    18241 ataaggcgtt cacgccgcat ccggcaacag gagccaaatg ccggatgcaa cgctggcgcg
    18301 ttttatccgg cctacaataa gctcaacata ctgatttgta tggttttgta ggccggataa
    18361 ggcgttcaag ccgcatccgg cgacaggaac caaatgccgg atgtgacgcg ggttctacaa
    18421 cgttaccggg tgttctttct cgcctttctt aaacttcact tccccgagct caatacaacc
    18481 caccgggcag acgtgaccac aaagcagaca acccacacat ttctcggtat tacaatgcgg
    18541 tgtgcgggtt ttctcgctcc attccatcgc ctggtgaccg ccgtcgtaac aggaaatata
    18601 acagcgtcca cagccaacac atttgtcaag attgatacgg ggatagacaa tataactgcg
    18661 gtctaaatct tccgccggga caatattgtt attcgccaga cctaccatct cctgcaacga
    18721 atcaaatccc tgatcggcga gataatgcga taacccgctc gccatatctt ctacgatgcg
    18781 attgccgtac tgcataatgc cggtggtgac ctgtaacgtt gctgcgccga gcaataaaaa
    18841 ctccgccgca tcctcccagg tttcaatgcc gccgataccg ctgattggga aatcgcgcag
    18901 ttctggatgg gtgcgcattt gctgaatgaa gcgcagggcg atcggtttta ccgctttacc
    18961 ggaatatccg gaaatactcg atttaccgtt aacgatcggc ataccgattt tctgattaag
    19021 atcgatattg gtgatggatt taacggtgtt aattgccgca atgccatctg cgccgccgcg
    19081 cttcgccgcc agcgccactt cgcacatatc accgatattc ggcgtcatct tcgccagcat
    19141 tggcagcgtg gaaccgcgtt tcaccgcccg acaatatttt tctaccagca ccggactttg
    19201 cccgacatcg ctacccatcg catgagaagt catttgcgga caggagaagt tacactcgat
    19261 catatccgcg ccagctcctc ccattgctgc tcattttccc ccatgatcga agc
//
LOCUS       DAJVUJ010000075        19154 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.075, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000075 DAJVUJ010000000
VERSION     DAJVUJ010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19154
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.075"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(<1..910)
                     /locus_tag="OQQ48_002327"
     CDS             complement(<1..910)
                     /locus_tag="OQQ48_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017441594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HCS1953079.1"
                     /translation="MTNFTTSTPHDALFKSFLTHPDTARDFMEIHLPKDLRELCDLDS
                     LKLESASFVDEKLRALHSDILWSVKTREGDGYIYVVIEHQSREDIHMAFRLMRYSMAV
                     MQRHIEHDKRRPLPLVIPMLFYHGSRSPYPWSLCWLDEFAAPTTARKLYSAAFPLVDV
                     TVVPDDEIVQHRRVALLELIQKHIRQRDLMGLIDQLVVLLVTECANDSQITALLNYIL
                     LTGDEARFKAFISELTRRMPHHRERIMTIAERIHNDGWLLGRERGRKEGKVEGERSLL
                     RLLLQNGADPEWIQRYTGLSAEQMQAL"
     gene            1377..2609
                     /gene="mdtM"
                     /locus_tag="OQQ48_002328"
     CDS             1377..2609
                     /gene="mdtM"
                     /locus_tag="OQQ48_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
                     /protein_id="HCS1953080.1"
                     /translation="MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFN
                     ADVSLAPAAVSLYLAGGIALQWLLGPLSDRIGRRPVLITGALIFTLACAATMFTTSMT
                     QFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAAL
                     MHFVHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVFRDFRNVFCNRLFL
                     FGAATISLSYIPMMSWVAVSPVILIDAGSLTTSQFAWTQVPVFGAVIVANAIVARFVK
                     DPTEPRFIWRAVPIQLVGLALLIIGNLLSPHVWLWSVLGTSLYAFGIGLIFPTLFRFT
                     LFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLA
                     GLLNRVRQHQAAELAEER"
     gene            2650..3930
                     /locus_tag="OQQ48_002329"
     CDS             2650..3930
                     /locus_tag="OQQ48_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HCS1953081.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDP
                     EMAARADAVKSYLKEDEAFNRYLSELWGDLREWLKADINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLSNF"
     gene            4046..5198
                     /locus_tag="OQQ48_002330"
                     /pseudo
     CDS             4046..5198
                     /locus_tag="OQQ48_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase subunit D"
     gene            5208..5976
                     /locus_tag="OQQ48_002331"
                     /pseudo
     CDS             5208..5976
                     /locus_tag="OQQ48_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313324.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5973..6230
                     /locus_tag="OQQ48_002332"
     CDS             5973..6230
                     /locus_tag="OQQ48_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HCS1953082.1"
                     /translation="MKEFLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGLTESIYCQQDGVWRCIAHYRVSPR"
     gene            6184..7155
                     /locus_tag="OQQ48_002333"
     CDS             6184..7155
                     /locus_tag="OQQ48_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiK family protein"
                     /protein_id="HCS1953083.1"
                     /translation="MVSGAALHITGFLRVKDSLRSLRILLDILLICLKGITVTKSISL
                     SKRISVIVILFAIVAVCTFFVQSCARKSNHAASFQNYHATIDGKEIAGITNNISSLTW
                     SAQSNTLFSTINKPAAIVEMTTNGDFIRTIPLDFVKDLETIEYIGDNQFVISDERDYA
                     IYVISLTPNSEVKILKKIKIPLQDSPTNCGFEGLAYSRQDHTFWFFKEKNPIEVYKVN
                     GLLSSNELHISKDEALQRQFTLDDVSGAEFNQQKNTLLVLSHESRALQEVTLVGEVIG
                     EISLTKGSRGLSHNIKQAEGIAMDASGNIYIVSEPNRFYRFTPKSSH"
     gene            7223..8395
                     /locus_tag="OQQ48_002334"
     CDS             7223..8395
                     /locus_tag="OQQ48_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1953084.1"
                     /translation="MPSSSHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVIMAE
                     GAFSFSQLSWIASGNYAGYLAGSLLFSFGAFHQPSRLRPFLLVSALARGLLILAMAWL
                     PPFILVLLIRFLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGIALGNEYVLAGL
                     HFAFSSQTLWQGAGALSGMMLIALTLLMPSKKHAIAPMPLAKTEQQIMSWWLLAILYG
                     LAGFGYIIVATYLPLMAKDAGSPLLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPCLT
                     ANLLVQAISVLLTLASDSPLLLIISSIGFGGTFMGTTSLVMTIARQLSVPGNLNLLGF
                     VTLIYGIGQILGPALTSMLGNGTSALAGATLCGAAALFIAALISTVQLFKLQVVTS"
     gene            complement(8408..8963)
                     /gene="kptA"
                     /locus_tag="OQQ48_002335"
                     /pseudo
     CDS             complement(8408..8963)
                     /gene="kptA"
                     /locus_tag="OQQ48_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151849.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
     gene            9213..9895
                     /locus_tag="OQQ48_002336"
                     /pseudo
     CDS             9213..9895
                     /locus_tag="OQQ48_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9892..10353
                     /locus_tag="OQQ48_002337"
     CDS             9892..10353
                     /locus_tag="OQQ48_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HCS1953085.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            10366..11538
                     /gene="iadA"
                     /locus_tag="OQQ48_002338"
     CDS             10366..11538
                     /gene="iadA"
                     /locus_tag="OQQ48_002338"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCS1953086.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGSKPGVTVFHMGDSKKALQPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            11603..12514
                     /gene="hypT"
                     /locus_tag="OQQ48_002339"
                     /gene_synonym="qseD"
     CDS             11603..12514
                     /gene="hypT"
                     /locus_tag="OQQ48_002339"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HCS1953087.1"
                     /translation="MDDCCAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEYGEAIFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDELVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(12507..12899)
                     /gene="iraD"
                     /locus_tag="OQQ48_002340"
     CDS             complement(12507..12899)
                     /gene="iraD"
                     /locus_tag="OQQ48_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HCS1953088.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(12896..12979)
                     /gene="idlP"
                     /locus_tag="OQQ48_002341"
     CDS             complement(12896..12979)
                     /gene="idlP"
                     /locus_tag="OQQ48_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HCS1953089.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            13571..14401
                     /locus_tag="OQQ48_002342"
     CDS             13571..14401
                     /locus_tag="OQQ48_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HCS1953090.1"
                     /translation="MSMPLSNALQSQIITDNHFLHHPKVDNKLTREYEYTRMDTENIY
                     LLPLTRGNNHNYDGKSVVEIRKLNIGKQSWPFNYATDTCREFNGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGYNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIGMGN
                     DPIIGIEAYPGTAEIHAKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(14542..15315)
                     /gene="uxuR"
                     /locus_tag="OQQ48_002343"
     CDS             complement(14542..15315)
                     /gene="uxuR"
                     /locus_tag="OQQ48_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HCS1953091.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(15530..16990)
                     /locus_tag="OQQ48_002344"
     CDS             complement(15530..16990)
                     /locus_tag="OQQ48_002344"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HCS1953092.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLKAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(17071..18255)
                     /gene="uxuA"
                     /locus_tag="OQQ48_002345"
     CDS             complement(17071..18255)
                     /gene="uxuA"
                     /locus_tag="OQQ48_002345"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCS1953093.1"
                     /translation="MEQTWRWYGPNDLVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            18595..18799
                     /locus_tag="OQQ48_002346"
                     /pseudo
     CDS             18595..18799
                     /locus_tag="OQQ48_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646216.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(19042..>19154)
                     /locus_tag="OQQ48_002347"
     CDS             complement(19042..>19154)
                     /locus_tag="OQQ48_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1953094.1"
                     /translation="GTSAVSLGLTANYARTGGQVTAGNVQSIIGVTFVYQ"
BASE COUNT         4477 a         4872 c         4805 g         5000 t
ORIGIN      
        1 ctaatgcctg catttgctct gccgaaagtc cggtatatcg ttgtatccat tcaggatcgg
       61 ccccattctg caacaacaat cggaggaggc tccgttcccc ttctactttc ccttctttcc
      121 tccccctctc ccttcccaac agccatccat cattatgaat tcgctctgca attgtcatta
      181 ttcgctccct gtggtgtggc attcgcctgg taagttcgct gataaacgcc ttaaaacgcg
      241 cttcatcgcc agtcagtaaa atgtaattta acagcgcagt tatctggctg tcattagcac
      301 actctgtaac cagtaatact accagttggt caataagccc catcaaatcg cgctggcgaa
      361 tatgcttttg gatcaactcc aacagggcga ctctgcgatg ctgcacaatc tcgtcgtctg
      421 gcacgacagt gacatccacc agcgggaacg ctgcgctata aagtttccgt gcggtagtcg
      481 gggcggcaaa ttcgtccagc cagcacaggg accaggggta aggactacgg ctaccgtgat
      541 aaaacagcat cgggataacc agcggtagcg gccggcgttt atcatgctcg atatggcgct
      601 gcatcaccgc catggaatat cgcatcaggc gaaatgccat atgaatatcc tcgcggctct
      661 gatgttcaat cactacgtaa atataaccat caccttcacg ggtctttacc gaccacagaa
      721 tatcggagtg tagcgcccgc aatttttcat cgacaaagct ggcggattcc agttttaagc
      781 tgtcgagatc gcacagttca cgtaaatctt tgggtaagtg gatctccata aaatcccgcg
      841 cggtgtcagg gtgagtgaga aaggatttaa ataatgcgtc atgtggcgtg ctggtcgtga
      901 agtttgtcat ggcgatccgt cacctgtaga taagatggcc cgactttagc gactaacaaa
      961 accattctca cccggcaatt tctcgttccc cgaggtgctt tccaggcgtc tgcaaacgat
     1021 acatttttcc ggaaacaggc tcgcgtttta catttcctgt acccggcaga tattcccctt
     1081 tccaccgcgc tcttttgatt aaccgcaaat tttaattaca cttaaggtgt atattttcta
     1141 tgcaacccat caattaaaga ggtgtaatgt gctgatgact atttgtaatc gttatacatt
     1201 ctgacctgga gtcagaaggt atttctctgt ctgtgtgttc acaggcagtg tggttgatta
     1261 catgaaattc agtacatttg cagtctcgtt gcccttctcg cctgcctttc gtcattaccg
     1321 acggtattga atttcgtttt ccccgttggg gttctccgaa caaggagttg tttgttatgc
     1381 cacgtttttt tgcccgccat gccgccacgc tgtttttccc gatggcgttg attttgtatg
     1441 actttgccgc gtacctgtcg acggatctga tccagccggg gatcattaat gtggtccgtg
     1501 attttaatgc cgatgtcagt ctggccccgg cagccgtcag tctctatctc gcgggcggta
     1561 tagcgttaca gtggctgctg gggccgcttt ccgacaggat tggccgcagg ccggtgctga
     1621 ttaccggggc gctaatattt acccttgcct gcgctgcgac aatgttcaca acgtctatga
     1681 cacagtttct tatcgcgcgt gcaattcagg gcaccagtat ctgttttatt gccaccgttg
     1741 gttatgtcac ggtgcaggag gcgttcggcc agacaaaagg gatcaagttg atggcgatta
     1801 tcacctccat cgtactgatt gcgccgatta ttggcccgct ttccggcgca gctctgatgc
     1861 actttgtgca ctggaaagtc ctttttgcca tcattgcagt tatgggtttt atctcatttg
     1921 ttggcttact gctggcgatg ccagagacgg tgaagcgcgg cgcggttcca tttagcgcca
     1981 aaagcgtctt tcgcgatttt cgtaatgtct tttgcaatcg gctgttcctc tttggcgcag
     2041 caaccatctc tttaagctat atcccaatga tgagttgggt ggctgtctcg ccggtgatcc
     2101 tgatcgacgc gggcagctta acaacttcgc agttcgcctg gacacaggtt ccggtgttcg
     2161 gcgcggtgat tgtcgcgaat gccatcgtgg cgcgtttcgt taaagatccg accgaaccgc
     2221 gctttatctg gcgtgctgta cccattcaac tggtcggcct cgcgctgttg attatcggca
     2281 atctgctgtc accgcacgtc tggctgtggt cggtgctggg caccagtttg tatgctttcg
     2341 ggattggttt gatcttcccg accttattcc gctttacgct tttttccaat aacttaccga
     2401 aagggaccgt ctccgcatcg ctgaatatgg tgatcctgat ggtgatgtct gtctcggtcg
     2461 aaatcggccg ctggctgtgg tttaacggcg gtcgcttgcc gtttcatctg ttagccgtcg
     2521 tggcgggcgt tatcgtcgtt ttcaccctgg caggattgct taatcgcgtg cgccaacatc
     2581 aggctgccga gctggcggag gagcggtgat ttttgcgcga tcctgccgtc aggctctatt
     2641 cttaacgtta tgaataaact cattgaactc agacgcgcca aaatgttggc gctctcttta
     2701 ctgcttatcg ccgctgctac ctttgtcgtt acgctgtttt tgccgcccaa tttttgggtg
     2761 agcggcgtga aggcgattgc tgaagcggcg atggtcggcg cgctggcgga ctggtttgcg
     2821 gtggtggcgc tgtttcgccg cgtgccgatt ccgatcattt ctcgtcatac ggcgattatc
     2881 ccgcgtaata aagaccggat tggcgaaaat ctcggtcagt tcgtgcagga aaaatttctc
     2941 gatacccagt cgctggtggc attgattcga cgtcacgaac cggcgttgtt gattggcaac
     3001 tggtttagtc agccggagaa cgcccgccgc gttggtcagc atctgttgca gatcatgagc
     3061 ggttttcttg aactgaccga tgatgcgcgt attcagcgcc tgcttaagcg cgcggtccat
     3121 cgggcgattg ataaggtcga tctttccgga accagtgcgt tgatgctgga gagtatgacc
     3181 aaaaacgatc gtcatcaggt gctgctcgat acgctgatcg cacagttgat cgcccttctc
     3241 cagcgcgata aatcgcgcaa gtttattgcc cagcaaattg ttcgctggct ggagagcgag
     3301 catccgctga aagccaaaat tttgcctact gaatggctgg gcgaacatag cgcggagttg
     3361 gtttctgacg cggtgaattc tttgcttgat gatatcagcc gcgatcgtgc gcatcagatc
     3421 cgtcatgcgt ttgatcgcgc cacttttgcc ctgatcgaca agctgaaaaa cgatccagaa
     3481 atggcagcgc gagccgatgc cgtaaaaagt tatctgaaag aagatgaagc ttttaaccgc
     3541 tatctcagtg aattgtgggg ggatttacgg gaatggctga aagcggatat caacagtgaa
     3601 gattctcgtg tgaaagaacg tatcgcgcgg gcgggtcaat ggtttggcga aacgttaatt
     3661 gccgatgatg ccttgcgggc atcgttaaat ggtcacctgg aacaagccgc gcaccgcgtc
     3721 gcgcctgagt tttccgcatt cctgacgcgc cacatcagcg atacagtaaa aagctgggat
     3781 gctcgggata tgtcgcggca aatcgagtta aatatcggca aagatctgca gtttatccgt
     3841 gtcaacggta cgctggttgg cggttgtatt gggctaattt tatatttgtt gtcgcagctc
     3901 ccggccttgt tccccctcag caatttttag aaatccatga aaaactaagt ggctaaccgc
     3961 gagagagatc aaataaacca cactaaaaga atggtaataa tgtggcctct tttcattatc
     4021 tcccgtggta cggggaagga aaatcatgtc acttatcacc gatctacccg ccattttcga
     4081 tcagttctct gaagctcgcc agaaaggctt tctcaccgtc atggatctca aggagcgcgg
     4141 cattccgctg gttggcactt actgcacctt tatgccgcaa gagatcccga tggcagccgg
     4201 tgcggttgtg gtttcgctct gttctacctc tgatgaaacc attgaagaag cggagaaaga
     4261 tctgccgcgc aacctctgcc cgctgattaa aagcagctac ggcttcggca aaaccgataa
     4321 atgcccctac ttctactttt cggatctggt ggtcggtgaa accacctgcg acggcaaaaa
     4381 gaaaatgtat gaatacatgg cggagtttaa gcccgttcat gtgatgcagt tgccgaacag
     4441 cgttaaggac gatgcctcgc gtgcgttatg gaaagccgag atgctgcgct tgcaaaaaac
     4501 ggtagaagaa cgttttgggc acgagattag cgaagatgct ctgcgcgatg ccattgcgct
     4561 gaaaaaccgc gaacgtcgcg cactggccaa tttttatcat cttgggcagt taaatcctcc
     4621 ggcgcttagc ggcagcgaca ttctgaaagt ggtctacggc gcaaccttcc ggttcgataa
     4681 agaggcgttg atcaatgaac tggatgcaat gaccgcccgc gttcgtcagc agtgggaaga
     4741 aggccagcga ctggacccgc gtccacgcat tttaatcacc ggctgcccga ttggcggcgc
     4801 agcagaaaaa gtggtgcggg cgattgaaga gaatggcggc tgggttgtcg gttatgaaaa
     4861 ctgcactggg gcgaaagcga ccgagctaat gcgtggcgga aacgggcgat gtctacgacg
     4921 cgctggcgga taaatatctg gcgattggct gctcctgtgt ttcgccgaac gatcagcgcc
     4981 tgcaaatgct cagccagatg gtggaagaat atcaggtcga tggcgtagtt gatgtgattt
     5041 tgcaggcgtg ccatacctac gcggtggaat cgctggcgat taaacgtcat gtgcgtcagc
     5101 agcacaacat tccttatatc gctattgaaa cagactactc cacctcagat gtcgggcagc
     5161 tcagtacccg tgtcgcggcc tttattgaga tgctgtaagg agtggcagtg acatattcga
     5221 ttggcattga ttctggttct accgccacca aagggatctt actggcagac ggcgtgatta
     5281 cgcgccgttt cctcgttcca acaccctttc gtccggcaac agcaattact gaagcctggg
     5341 aaactctgcg cgaagggtta gagacaacgc cgtttctgac gctcaccggc tatggacggc
     5401 aactggtgga ttttgccgat aaacaagtga cggaaatctc ctgtcacggg ctgggcgcac
     5461 ggtttcttgc gccagcaacg ctgcgcggta atcgacatcg gtggtcagga cagcaaagtg
     5521 attcagcttg atgatgacgg taacctgtgc gatttcctga tgaatgacaa atgcgcggcg
     5581 ggcaccgggc gtttcctgga agtgatctcg cgcacgcttg gcaccagcgt cgagcaactc
     5641 gacagcatta ccgaaaatgt cacgccgcat gccatcacga gtatgtgcac agtgtttgcc
     5701 gaatcagaag tgatcagcct gcgctcagcg ggcgtcgcgc cagaagcgat tctcgccgga
     5761 gtgattaacg cgatggcgcg gaggagtgcc aatttcattg ctcgtctctc ctgtgaagcg
     5821 ccgattctgt ttactggtgg cgttagtcat tgccagacgt ttgcccggat gctggaatct
     5881 cacctgggaa tgccggtaaa tacccatcct gatgcgcaat ttgctggcgc aattggcgcg
     5941 gcggtaattg gtcaacgagt gaggacacgc cgatgaaaga gtttttattt ttatttcact
     6001 ccacagtcgg cgtcatacaa actcgcaaag cgttgcaggc agcgggcatg acttttcgcg
     6061 tcagcgatat tccccgtgat ttacgcggcg gctgcgggtt atgcatttgg ctgacctgtc
     6121 cccccggcga agaaatacaa tgggtgatcc ctgggctgac tgagtcaatt tattgccagc
     6181 aggatggtgt ctggcgctgc attgcacatt accgggtttc tccgcgttaa ggattcatta
     6241 aggtcgctac gtatacttct cgatatctta ttgatttgcc ttaaagggat taccgtgaca
     6301 aaaagtattt cgttaagtaa acgcatctcc gttattgtga ttctttttgc cattgtggca
     6361 gtgtgtactt tcttcgtgca atcttgcgcc cgtaaaagca atcatgcagc atcatttcag
     6421 aattaccacg ccaccattga cggcaaagaa attgcaggca taacgaataa tatctcttcg
     6481 ctgacctggt cggcacaaag caatactctt tttagcacta tcaacaaacc tgccgccatt
     6541 gttgaaatga ccaccaatgg tgattttatc cgcacaattc ctctggattt tgttaaggat
     6601 ctcgagacga tcgaatacat tggcgataat cagtttgtca ttagcgatga gcgcgattac
     6661 gccatttatg tgatttcact gaccccaaac tcggaagtaa aaatcctcaa aaaaataaaa
     6721 atcccgctgc aagactcccc aaccaattgc ggttttgaag ggttagctta ttctcgccag
     6781 gatcacactt tctggttttt taaagagaaa aatccgattg aggtctacaa agttaacggg
     6841 ctgttaagta gcaatgaatt acacatcagc aaagacgagg cactacaacg gcaatttact
     6901 ttagatgatg tgtccggcgc agaatttaac cagcaaaaaa atacgcttct ggtgctgtct
     6961 catgaatctc gcgcactcca ggaagtaacc ctggttggcg aggttatcgg tgaaatatca
     7021 ctaaccaaag gtagtcgagg tttgtcgcat aacatcaaac aggccgaagg gattgcgatg
     7081 gatgcctctg gtaatattta tatcgttagc gaaccaaatc gtttttatcg ctttacccct
     7141 aaatcatcgc attaacaggc tggcatattt cacctttttg ttattaactt gttaacaaca
     7201 aaaataacat acaaggttaa acatgccttc gtcctcgcat cccgtagaac gcttttcttt
     7261 cagcaccgcg cttttcggga tgctggttct gaccctaggt atgggattag gtcgcttcct
     7321 ttatacgcct atgttgcccg tcattatggc ggaaggggcg ttttcattta gccagctctc
     7381 gtggattgcc agcggcaact atgcggggta tctggctggc agtctgctat tttcgtttgg
     7441 cgcatttcac cagccgtcgc gcctacgccc atttctgttg gtttctgccc tggcgagagg
     7501 gttgttgatc ctcgcaatgg catggttgcc gccatttatt ctggtgttat tgattcgctt
     7561 cctggcgggt gtcgccagcg ccggtatgct gatttttggt tcgacgctga ttatgcagca
     7621 cacgcgccat ccttttgtgc tggcagcttt gttttctggc gttggcattg cactgggcaa
     7681 cgaatatgtt ctggcaggcc tgcattttgc cttctcctcg caaacgttat ggcaaggcgc
     7741 cggggcgctt tctggcatga tgctgattgc acttacgctt ttaatgccct cgaaaaaaca
     7801 cgccatcgca ccaatgccat tggcaaaaac ggagcaacag ataatgagct ggtggttact
     7861 ggctattctg tacggccttg cgggatttgg ttatatcatc gtcgcgactt acctgccgct
     7921 catggcgaaa gatgcaggct caccattgtt aaccgcccat ctctggacgt tagtcggctt
     7981 atcaattgtg ccaggctgct ttggctggct atgggcagca aaacgttggg gggctttgcc
     8041 ttgcctgacg gcgaatttgc tggtgcaggc tatcagtgtg ctgcttactc tcgccagcga
     8101 ctctcctctc ttgcttatca ttagtagtat cggttttggc ggtaccttta tgggcacgac
     8161 atctctggtg atgactatcg cccgccaact gagcgttccg ggaaatctca atcttttagg
     8221 ctttgtgacg ctcatttacg gcattggaca aatcctcggc ccggcgctga ccagtatgct
     8281 cggcaatgga acatcggcgc ttgccggcgc cactctctgc ggcgcggcgg cgttgtttat
     8341 tgctgcgctt atcagcacag tacaattatt taagctgcaa gtggtcactt catgaggagt
     8401 gtttccctta ccactcaaga aattcgaccg caaccgttga tgtcagccag actccgtttt
     8461 ccgcttgcca gaaaggaatg cctcgtttcg ccatttcctg tgctttgacg gttaagacaa
     8521 ccggcgatcc gtgtcttgct cccactttgc gtgccgttgc ttcatcagca gagagatgca
     8581 cgtaatgacg atcccctgca atcagcccct gtttttttta tctcgtcaag gaaacggctt
     8641 gccgtaccgt ggtagagaaa ctgtggcggt gttttttcgg caaaggaaat agcgacctgc
     8701 gaagtcgaat gcccctgaac cgcgcggatg cacctaccat cactggaata actaaaacgc
     8761 tttttatcgc tggttgcgac cacggtatcc agcaatgcac gggttaatcg cttgcccgct
     8821 ttttgggcac aaagtatgag cttatcgata tccgcccatc cttcacggtc cagtacgata
     8881 ccaatcgcct ccggtttgtg tcggaggaca aaacttaaaa acttgctcgt atccgccagc
     8941 tctttttcgt tgtgctttgc catctcgttt tcgtccctgg attttctgtt tttatttacc
     9001 aatgtcattg tcactgggtt tatgaatttt ctgcaactca atcctgcgca gaaccttttc
     9061 cacacagcgt aatgtttccc ctatgcaaaa aacgcatagc cagaaacata cttgcatttt
     9121 ccccataccc acatggctga taaggttttt cagttccagg aattttcctg gctcaagcaa
     9181 cacacaaaca caaaaaaaca atggggattg ttatgacaca acaaggggat gctgtagccg
     9241 gggagctggc gacagaaaaa gtgggcatta aagggtatct ggcttttttt ctcaccataa
     9301 tcttttttcc ggtgtttttt ccggaacaga cagctggtgg cgcgtttttg atttttccgt
     9361 tctgaacgga tcgtttgggc aactgcctgg tgcgaacgga gccaccacct catttcgcgg
     9421 cgcgggtggt gcgggggcta aagatggctt tctctttgcg ctggagctgg caccgtcagt
     9481 cattctctct ctggggatta tttcaattac cgatggtctt ggtggattac gcgccgcgca
     9541 gcagttaatg acacctgtgc taaaaccgtt gttgggcatt ccagggatct gctcgctggc
     9601 gctgatcgct aacttgcaaa acactgatgc cgccgccggt atgaccaaag agctggcgca
     9661 ggaaggtgaa attaccgaac gcgataaagt gattttcgcc gcatatcaaa ccagcggtag
     9721 cgccatcatc accaactact tctcttccgg cgtcgcggtg ttcgcctttc tgggcacgtc
     9781 cgtaatcgta cctttagccg tcatcctggt gtttaaattt gtcggagcca acattttgcg
     9841 cgtctggctt aacttcgaag aacgtcgcaa cccaacgcaa ggagcgcaag catgacaact
     9901 caggtccgca aaaacgtcat ggatatgttt atcgatggcg cgcgccgtgg ctttactatc
     9961 gccacgacca acctgctgcc aaacgtggtg atggcgtttg tcatcattca ggcgctgaaa
    10021 attaccggcc tgctcgactg ggtggggcat atctgtgaac ccgtaatggc gctgtgggga
    10081 ttacccggtg aagcggcaac cgtcctttta gccgcattaa tgagtatggg gggcgcagtc
    10141 ggtgttgccg ccagtctggc aaccgctggc gcattaaccg gtcacgatgt caccgtttta
    10201 ctgcccgcga tgtatttaat gggcaacccg gtgcaaaatg tcggtcgctg cctggggaca
    10261 gccgaagtga atgccaaata ttacccgcat atcattacgg tctgcgtgat taacgcattg
    10321 ctgtcgatct gggttatgca gcttattgtt taacaaggag ttaccatgat tgattatacc
    10381 gcagccggtt ttaccctgct gcagggagcg catttgtatg cgccggaaga tcggggaatt
    10441 tgcgatgtcc tcgtcgctaa cggcaaaatt atcgccgttg ccagcaatat cccttctgac
    10501 attgtaccga actgcacggt tgtcgatctc agtgggcaga tcctctgccc aggttttatt
    10561 gatcaacacg tccatttgat tggcggtggc ggcgaagcag gtcccacgac gcgcacgccg
    10621 gaagtggcgc taagtcgcct gacggaagcg ggcgtcacgt cagtggttgg tctgctgggc
    10681 accgactcta tctctcgcca cccggaatcc ctgctcgcca agacccgtgc gctcaatgaa
    10741 gaaggcatca gcgcctggat gctgaccggc gcttatcatg tcccttcccg cactattacg
    10801 ggttccgtgg aaaaagacgt ggcgattatc gatcgtgtga ttggcgtgaa atgcgccatc
    10861 tctgatcacc gttctgccgc accggacgtt tatcacctgg ctaatatggc ggcagaatcc
    10921 cgcgttggcg gtttgctcgg cagtaaacct ggcgtcaccg tgttccacat gggcgacagt
    10981 aaaaaggcgt tacagcctat ttatgacctg ctggaaaact gcgatgtgcc gatcagcaag
    11041 ctgctgccga cccacgttaa ccgcaacgta ccgttgtttg agcaggcgct ggagttcgcg
    11101 cgcaaaggcg gcaccatcga tatcaccagc agcattgacg aaccggtcgc ccctgccgaa
    11161 ggtattgccc gcgccgttca ggcgggtatt ccgctggcac gcgtcaccct cagctccgac
    11221 ggcaacggta gccagccgtt cttcgatgac gaagggaatt taacccatat cggtgttgca
    11281 ggttttgaaa cgttgctgga aaccgtgcag gtgctggtca aagactatga tttcagtatc
    11341 agcgatgccc tgcgcccgct caccagtagc gtagccggtt tccttaacct gagcgggaaa
    11401 ggcgaaattc tgcctggcaa tgacgcagac ttactagtca tgacgccaga attgcgcatt
    11461 gagcaggtat atgctcgcgg caaactgatg gtcaaagacg gcaaagcctg cgtgaaagga
    11521 acgtttgaaa cggcttaaac ggttgtcagc cgccctaatg gacgtagaat gcccgcaagg
    11581 gcggctgaca gagtaaaacg taatggatga ctgttgtgcg gttttgcata atattgaaac
    11641 caaatggctt tatgattttc tgaccctgga aaaatgccgc aatttttccc aggcggcagt
    11701 cagtcgtaac gtctcgcaac cggcattcag ccgccgcatc cgcgcgctgg aacaggcgat
    11761 tggcgttgaa ttgtttaacc gccaggtgac gccgctgcaa ctctcagaac aagggaaaat
    11821 cttccattcg cagatccgcc atctgttgca acagttagaa agtaaccttg cagagctgcg
    11881 tggcggcagc gattacgcgc aacgtaaaat caagatagcc gctgcacact ctctttccct
    11941 cggtctgtta ccgtccatta tcagccagat gccgccgctc tttacctggg cgattgaagc
    12001 tattgatgtc gatgaagcag tcgataaact gcgtgaaggg caaagtgact gtattttttc
    12061 ctttcacgac gaagatttgc tggaagcacc gtttgaccac attcgcttat ttgaatctca
    12121 attgttcccc gtttgcgcca gtgacgaata cggagaagca atttttaacc tcgcgcagcc
    12181 acactttccg ttactgaatt acagccgcaa ctcctacatg gggcgattga ttaatcgcac
    12241 cctgacgcgc cacagcgagt taagtttcag cacctttttt gtctcttcga tgagcgagct
    12301 attaaagcag gttgccctcg acggctgtgg gattgcctgg ctgccggagt atgccatcca
    12361 acaagaaatt cgcagcgggc aactcgttgt gctgaatcgg gacgaactgg tgatcccgat
    12421 tcaggcctac gcataccgta tgaatacccg catgaatcca gttgccgaac gcttctggcg
    12481 tgaactgcgc gagctggaga ttgtgcttag ctgacattct caagcgtcgc actgcgccaa
    12541 ccgccattat cccagaccag atcaaacgtt agcggttcat cgtcgtggta cagcattatc
    12601 tcaaggctga tcgccagagt gtggcagtac gcttctttca aattaacctg caacgccgca
    12661 atccgtggct catgaaataa cagtgcagaa cgcagctgac ggcaatacca gctctggaga
    12721 tgtttgtcgc tcgggttatg cacttgccac tcgggcatgc ctgaaacaag taatgacggt
    12781 aatgccgaat gaggagaatt aaaaagagtg aggagatcgc tgcataccga tttacgcagc
    12841 aatgacgttt tattgcctga aatttcaggt aaaacagcct gaagtgattg gcgcatcatt
    12901 ttgcgcactc ctgacgttta gcaacatatg cggcttgccc tgaacaccgg gcaccactgt
    12961 attgatgctc attttccata gccattccct tttgttcatt acatccatgt cagtctgaga
    13021 caggcctgaa tcctgtcagc aaagtataaa accttacttg cctatgaata tctactgact
    13081 tttcaggaaa aaagtcatga tttgataaaa atcatcaaac tcaaaaaaaa accgctagta
    13141 tattctgaat attccgaata gttacgtaga tgaaaatact tgtcaccgcc gctacttaat
    13201 tccggtggga aattatttat ttccgcataa tcagccatgt attatttgaa acaattagtt
    13261 tgtaaataat ataaattgat aacatatcaa atacatcaaa aataccattc ataggcatta
    13321 ttaatataga catcaatata gcaacatcaa attccaatta gtaataagat atgcttttta
    13381 attgcatgaa aataatttca aatgagcgtt ttttgatact ttagagtgtt tcctaccctt
    13441 taatatcacc gtgtagagtt gatatcacta caacagaaac atgcatgtca tttttttact
    13501 tcttttttac tacagaaaaa tttatctatt tttctaaaca tcggtgaaat actcataaaa
    13561 ggtaactatt atgtctatgc cattaagcaa tgcccttcag agtcaaatta ttacagataa
    13621 tcacttcctt catcatccaa aagtcgacaa taagttaaca cgtgaatatg aatatacacg
    13681 catggatacg gaaaatatct atcttctgcc tcttactcgt ggaaataacc ataattacga
    13741 tggaaaaagc gttgtcgaaa ttagaaaact taatattggc aaacagtcct ggccatttaa
    13801 ttatgcgaca gatacatgca gagaattcaa tggtattaca acaacaggac gtatgctcta
    13861 tcgaaacttg aaaatcacct cagctttaga cgaaatatat ggtggcatat gtaaaaaggc
    13921 gcatgctgct acagagttag cagaaggttt acggctaaat ttatttatga agagcccttt
    13981 cgatcctgtt gaggactata cagttcatga aataacatta ggccctggtt acaatgttcc
    14041 tggttatgcc ggaaccacga taggatatat ttcaaccctt ccagcttctc aggcaaaaag
    14101 atggaccaat gagcagccaa gaattgatat ctatattgat cagataataa ctgtctcagg
    14161 ggtggcaaat tcttccggat tcgcccttgc cgcattactt aatgctaata ttggaatggg
    14221 aaatgaccca ataattggta tagaagccta tcctggtacc gcggagatcc atgcaaaaat
    14281 gggatataac gttatccccg gtgatgaaga tgcgccactc aaacgcatga ctctgcaacc
    14341 gtcatctctt ccagaactgt ttgaattaaa aaatggtgaa tggaattata ttggcaaata
    14401 accactgaga agaatgagta ctaacattca ggttatatag atatttaaac aataccctgc
    14461 aatttacaaa gtaatactca ttgcgacttc ttaatttaag aaagcgcaag gaacgtttac
    14521 ccttgcgctt atcataatca gtcaggcgtc gactttatcc agcggccatg aatcaaacag
    14581 atagccgtca aaataaatat cgtcaacgtt cgagaattcc agcagacgtt gcttaacgtt
    14641 ttccagatgc tgccacattg ccagcttcgc cgctcgggca tcttttttga ttaacgcggc
    14701 aaggatctgt ttgtgatcgc ccaaccactc tttgcgatac aggctgtcat ccagatggct
    14761 gtgcaactgg atccacattg gattgttttc ccgccactgc caggactgac ggaataactc
    14821 caccagcatg ctgttatgcg ttgcttcagc aatggcgaga tggaactgca tgtcaccgct
    14881 ttcgctgcta cccggcgcgc tggaagccag ttcacgctct tccagttgca atgcctgacg
    14941 cattttgacg atatcttcgc gggtagcctg cagggcggca aattccgcga tgttgctctc
    15001 aagaagctgt cgcgcctgca acaattcaaa ggggccggca tcattgcaga cgttggcatc
    15061 cggactgtct gtgttgtggc tgcctgagct atcaagaaca tagataccgg caccccggcg
    15121 cacttccacc agccctttga tctccagcat gatcagcgct tcacgtacca ccgtccgcgt
    15181 gacatcaagc atttctgcaa tttcacgctc cggcggcagc cgttcgccag gattgtacgg
    15241 cgtctttatg atcagatcgc ggatcatcgc cccgacttcc tggtaaggtc tttgcgcaga
    15301 ggtggcagat ttcatggtgt tgtgtccgtt aggtgcggtt aatcaaattt ttgccggatg
    15361 cggcgtgaac gccttatccg gcctacaacg ggcaatttta cggcccccgt taatctcaaa
    15421 ctataccatt agcactggaa gttagcacgt acaggtgggc aaaaaaacga aaactggtca
    15481 accaaatatc agtttgattg accagtaagt ctgtagggta atcagttagt tacagcgcag
    15541 ccacacactc gcgcgcaccg cgttcgcaca gctgctgata tgccgccgtc accgcgccaa
    15601 caaagtcggc attctgcggc agatcatcgg caaaaatacc gctcaggccc agcagcgctt
    15661 tcacgcggtc tgcgccctga tactgcgcgt taatcttctg gaactcggcc agcatcgggt
    15721 cgaccacgtc aatggcatta ccctgctcat ccacgccctg ggtgtaacgc atccagccag
    15781 ccacgcccag cgccaggtga cgccagctgc cgccattttg caggtgcaga cgcaccgggt
    15841 ccagcagacg ctgcggtaac ttctggctgc cgtccatcgc aatctgccag gtacggtgac
    15901 gcagagacgg gttgctgaaa cgctcgatca gcagcgtcgc ataggccttc aggtccgtac
    15961 cctccggcat agacagcgtt ggcgcttgtt cctgcatcat cagggcaaag gccgctttgc
    16021 gataatccgg gttagtcatg gtgtcggcaa tagtttcata gccgccgagg taaccgaggt
    16081 atgccaggaa agagtggctg ccgttcagca tacgcagctt catcatttcg aacggcacaa
    16141 cgtctgcaac gaactgtgcg cccactttat cccagtccgg gcgaccatta acgaagttat
    16201 cttcaatcac ccactgacgg aacggttcgc aggcaatggc gcacgggtcg taaacaccca
    16261 gctggtcagc aatttcctgt aaggtttctg gcgtcgccgc cggaacgatg cggtcaacca
    16321 tggtgcacgg gaaggtgacg ttctcttcaa tccatgccgc cagctgcggg tcacgtgcct
    16381 gagccagccc cagtaccgcg accttcgcca catgaccgtt ttcacgcacg ttatcgcagg
    16441 acatcaccgt aaatgctttc agcccttttt cacgacgcag acgcaaggct tcgacgatgt
    16501 aaccaatcgc ggacttcggc gcagtcgggt tttctagatc gtgcttgatc agcgggttat
    16561 tgagatccag ttgaccgctt gccgcatcag cgcagtagcc tttttccgtg accgttagag
    16621 agacaatcgc cgtttgcgga cgcgccatcg cgttgagaat accttcgcag ccgtcgattt
    16681 ccggatgcag cgcttctttc atcgaaccga taattttcag ctcggtgctt tctgcgcctt
    16741 tttccgctac ggtgtacagc agttgctgtt ttttcaggtt ttcgatcagc acgcggtcgt
    16801 tgcctggcat caggttaact tcgcagatgc cccagtcgct gtcggtgctt tccagcagat
    16861 gatgggtata cagcgcctga tgtgcgcggt gaaacgcccc gcaaccgaga tgcacaatgc
    16921 gtgattccag acgagaatga tcccatgacg gacgggcaac cggcagattg ctgtcaacaa
    16981 tagtagtcat cgtaaactcc agaatccgtc ttcagagagt gacggcaagg aagaaggaac
    17041 cggcacggcg cgcagccatg ccggtggata ttaacggcta aagaaagcgc gctggatcgc
    17101 cagttcgaca ccgcgaactt cggccaggcc tttcagacga ccaattgcgg agtaacctgg
    17161 gttggttttc ttcttcaggt cgtccagcat ctgatgaccg tggtccggac gcatcgggat
    17221 caggtcttct ttgccttccg ctttacgacg gtgttcttct tcaacaatcg ctttcaccac
    17281 ttcgtacata tcaacgtcac cgttcaggtg cgccgcttcg tggaaggttt tcgggttatc
    17341 ttcacgcatg gtggagcgca gatgggtgaa gtaaatacgc ggaccgaact gcttgatcat
    17401 atcaaccaga tcgttgtcag cacgcacgcc gtaggaaccg gtgcacatgg tgaaaccgtt
    17461 cgccatgctg tttacggtat caaccatcca ctgcatatct tcaatggtgg aaacaatgcg
    17521 cggcaggccg aggatcgggc gcggcggatc gtccgggtga accgccatac gcacgccaac
    17581 ttcttcagca actggaataa tcgctttcag gaagacggca aagttttcgc gcagcttcgc
    17641 tttatcgata tctttgtaca gctccaggtg cttacggaac tggtcgaggg tatacccttc
    17701 ttccgcgccc ggcagaccgg caatgatgtt acgcgtcaga cgcgctttgt cttcatcgct
    17761 catagtggcg aagcgttcag ctgcctgagc aatttcttct tcggtgtaat ccgcttccgc
    17821 gcctggacgt ttcaggatat gcatttcgaa tgcagcgaat tcgatctggt cgaagcgcag
    17881 agctttggag ccgtctggca gcacgtattc gaggtcagta cgggtccagt cgagcaccgg
    17941 catgaagttg tagcacacgg tgcgaatgcc gcactgcgcc aggttgcgca gagtctgctg
    18001 atagttagcg atccactgct catagttgcc agtgtgggtt ttgatatctt cgtgaattgg
    18061 tacgctttct acgacagacc acaccaggcc tgcgtcttca acgatcgcct tgcgtttgag
    18121 gatctcttct acggaccata cttcgccgtt cgggatatgg tgcagcgcgg taaccacgcc
    18181 agttgcgccc gcctgacgga catcagctaa agaaaccaga tcgtttgggc cgtaccagcg
    18241 ccaggtctgt tccatgattt ttcctctttc acatagtggg ttccacagca aagagacgcg
    18301 gaaccctgga gttgtttagg tatccggatg aacaaccaga ccgcagccgt ggcgttgcat
    18361 cggatgagat ggcgtataag ttctaccttt tggtaagata tatcaaccca aatcctgaaa
    18421 ttggttaacc aaatcacaag aatttcactc tcatggttgt tgctgcaaat tgcagcaaaa
    18481 ggtaatcgat ggttattgat cagaggatgt gacattcatc gcaacaatgg ttgaccaatt
    18541 tacataacat gtcgcccaaa ataacaacgg ttcaaccagg taaggtaaaa cgttatgcat
    18601 gtgcttaaca ttctctgggt agtattcggc attggtctga tgctggtact gacaacgacc
    18661 gcaggttcag acacattcac tgcattagca atgcctctta gattattttg acctgtcagt
    18721 aaaagacacg ttgaaaacct ggggtctgct ggaactggtc aactccgtgg ttgggctgat
    18781 tattagcaag gtagcgtaaa agaacaccag agcctgccaa tggcaggctc tgactgatga
    18841 caaagtctaa aaatgtgccc ggacagtccc ctcgcccctc cggggagagg gttagggtga
    18901 ggggaacaga ccagcaaagt gcgctttgtt tatgccagat gcgacgctga cgcgtcttat
    18961 ctggcctaca aagggctaac gtgcaggttt ttagcttcag gtaatattgc gtaactgcat
    19021 tagcaatgcc ctgtgatttc tttattgata aacaaaagtc acgccaataa tcgattgcac
    19081 attccctgca gtcacctgcc ctccggtacg tgcgtaattt gccgttaatc ccagacttac
    19141 cgccgaagtt ccta
//
LOCUS       DAJVUJ010000076        18921 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.076, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000076 DAJVUJ010000000
VERSION     DAJVUJ010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18921
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.076"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..1439
                     /locus_tag="OQQ48_002348"
     CDS             <1..1439
                     /locus_tag="OQQ48_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Tn3-like element TnAs1 family transposase"
                     /protein_id="HCS1953095.1"
                     /translation="NSDQYLEERLQLLDEQLATVTRLAKDNELPDAILTESGLKITPL
                     DAAVPDRAQALIDQTSQLLPRIKITELLMDVDDWTGFSRHFTHLKDGAEAKDRTLLLS
                     AILGDAINLGLTKMAESSPGLTYAKLSWLQAWHIRDETYSAALAELVNHQYRHAFAAH
                     WGDGTTSSSDGQRFRAGGRGESTGHVNPKYGSEPGRLFYTHISDQYAPFSTRVVNVGV
                     RDSTYVLDGLLYHESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGETKLYV
                     PQGVQAYPTLRPLIGGTLNIKHVRAHWDDILRLASSIKQGTVTASLMLRKLGSYPRQN
                     GLAVALRELGRIERTLFILDWLQSVELRRRVHAGLNKGEARNSLARAVFFNRLGEIRD
                     RSFEQQRYRASGLNLVTAAIVLWNTVYLERATQGLVEAGKPVDGELLQFLSPLGWEHI
                     NLTGDYVWRQSRRLEDGKFRPLRMPGKP"
     gene            complement(1743..3089)
                     /locus_tag="OQQ48_002349"
     CDS             complement(1743..3089)
                     /locus_tag="OQQ48_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001632353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1953096.1"
                     /translation="MPMILVKKNPRGKVIRELSSEEETAVKTVCGLKKPATMALHNLA
                     NDILREMREYDAWLQCDCIPGDSPAMNFAALKNNTGTLYLSSFNHEHAPECPMYRQLS
                     GNEEETYSGASRHPVSTRINYRNFLPPDDSNSVIRLQARSAYHNGESSSVRKKRPRLA
                     RLLLSLIDDAGLNKLDSLANPRIRTNYRECLDAIRQVTLQQEYIRGRSLSEIIHFRPG
                     MSERSQERLMETLENSERHWPARRKHMFFQIFMAQHICRDAVEIHWANGNIQVIRPVR
                     GISINGEAQGGIRPPYWVILAFCRSADGRIICSEGYAHALYQLTCPVPVDSKLERNTL
                     TALLNVASWLKRKPGTPELSLERPLFDTEVYVNGEKKYVLPDFIVTARAPDGKTARVV
                     IETMGYEDSDYCARKSRQHTGMKQIGVLHTDPPKWLDNDHPPFEKHMYGVFMHLRY"
     gene            3443..4045
                     /locus_tag="OQQ48_002350"
     CDS             3443..4045
                     /locus_tag="OQQ48_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proQ/FINO family protein"
                     /protein_id="HCS1953097.1"
                     /translation="MGKNQEERKTPVIVVKKRRTFSPPSLSEKTDIIAPVFTEQTAES
                     APAGINSSAVETHIPEAPARKKKKKRHRFPRPSHWTREYTHECVEKIKALFPHLRAEG
                     GGFIPLKIGINNDISAFLAEHPETELTMDEWLCAVSCITSRRVYLQRTAVAGVPRYGL
                     DGHPKGQVSDSEAQSAGRRLATLEQKWLRMQAQQENISGQ"
     gene            4101..4283
                     /locus_tag="OQQ48_002351"
                     /pseudo
     CDS             4101..4283
                     /locus_tag="OQQ48_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001582574.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4283..4549
                     /locus_tag="OQQ48_002352"
     CDS             4283..4549
                     /locus_tag="OQQ48_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1953098.1"
                     /translation="MITGNGINTVTVNGKVKHITELDDITLCLEWTKLREENNRLYEI
                     NNQANRGWRGFILRLIGVNLPDKRTEFTQRLLLTRKISGSVMKK"
     gene            complement(4740..5021)
                     /locus_tag="OQQ48_002353"
     CDS             complement(4740..5021)
                     /locus_tag="OQQ48_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1953099.1"
                     /translation="MNYDEITKITAERISDYMTEAVNTDSIAVAEMFHNAAWGVRTLW
                     FELVTKIDIDIHKKNRYASYDLRRKIEMQHEEFQKMTEREQVPLLKSPE"
     gene            complement(5127..5402)
                     /locus_tag="OQQ48_002354"
     CDS             complement(5127..5402)
                     /locus_tag="OQQ48_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCS1953100.1"
                     /translation="MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIYVLRLWHTRENR"
     gene            complement(5402..5686)
                     /locus_tag="OQQ48_002355"
     CDS             complement(5402..5686)
                     /locus_tag="OQQ48_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HCS1953101.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWIIKQA
                     LSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAWADSLSTDNPLPVPR"
     gene            complement(6591..7463)
                     /gene="repA"
                     /locus_tag="OQQ48_002356"
     CDS             complement(6591..7463)
                     /gene="repA"
                     /locus_tag="OQQ48_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HCS1953102.1"
                     /translation="MAALNQTSFTESTYRQVKNPNPVFTPREDAGTLKFCEKLMEKAV
                     GFTSRFDFAAHVAYARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTL
                     AIECGLATESEAGKLSITRATRALKFLSELGLITYQTEYDPTIGCNIPTDITFTPALF
                     DSLDISEEAVASARRSRVEWENRLRKKQGMDALGMDELIARAWRFVRERFRSYQAELK
                     SHGMKRARARRDAGRTRQDIVTLVKRQLTREIAEGRFRGSLEAVKREIDRRVKERMIM
                     SRNNNYTRLATASP"
     gene            8166..8807
                     /locus_tag="OQQ48_002357"
     CDS             8166..8807
                     /locus_tag="OQQ48_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusG"
                     /protein_id="HCS1953103.1"
                     /translation="MCLSELQARLNVGQPLFPTHALCIRRSQQHRSPPAGIDFRGAFR
                     ILNISELHQRNWYLAQYIPTGKNREHLFSWLSEQHVLPWTPLILKKVRRTDKVCGYRR
                     HIHAVFPGYFFLKADPESHSFTHLRRHSAFLDFVKMAGEIKTVREDIVQSLMKVYPDP
                     ALNPAAREELDAASTLWLTKARYQYLLRLDAQPLPESRIALLLHLVSDDGALT"
     gene            8947..9609
                     /locus_tag="OQQ48_002358"
     CDS             8947..9609
                     /locus_tag="OQQ48_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="traC"
                     /protein_id="HCS1953104.1"
                     /translation="MTIQSPENPVIQLLENMLEALSTSPDSINNERRRRRFLLNWLDS
                     ARQMSEFRGMPEEFTALRKLLATNTDIPVENTLKSLLESGHNAALYDLYRFRAALATC
                     RSLGWQIGTCAWNDQLLSETLSRAQTGKRHILQLNSTGDTFSTTGRMIRPVAFSLIHP
                     ATINTSEVEEIFRDEGFMLAHGRECSLNGSGRNMLSRILHVGHSGLAEEEWGMDNSLL
                     LH"
     gene            9876..10019
                     /locus_tag="OQQ48_002359"
     CDS             9876..10019
                     /locus_tag="OQQ48_002359"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1953105.1"
                     /translation="MTLTLLTAYNAPYLAALTRPLVMPAPEKVPDVSEPVIAPDNSEP
                     QQV"
     gene            10100..10342
                     /locus_tag="OQQ48_002360"
     CDS             10100..10342
                     /locus_tag="OQQ48_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilI type IV pilus biogenesis protein"
                     /protein_id="HCS1953106.1"
                     /translation="MSAEKFRVVIYTNTCEKKVSYISATEDINIAIMPFQTADNGLII
                     VCQNSRIIRRKERIPGSSCEWKDVTLAPKSRNRVQL"
     gene            10458..10778
                     /locus_tag="OQQ48_002361"
     CDS             10458..10778
                     /locus_tag="OQQ48_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1953107.1"
                     /translation="MRTITTREQLLINGRVHERIATHIVTGAHGYETLCTNGYNVRYN
                     KAQQLVESCEKIAEGKLPVTCPVCFTIWQDVHRFTHVDFDTQSGKSDFIDTCHSEIIT
                     GMFQ"
     gene            11019..12089
                     /locus_tag="OQQ48_002362"
     CDS             11019..12089
                     /locus_tag="OQQ48_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpQ domain-containing protein"
                     /protein_id="HCS1953108.1"
                     /translation="MKKKSLTLLLPSLLSGCTAFNATPVPDTTPPALVFVDGQIRESA
                     AIIAHTQKRLLPPPVTRPVTPPRPTTTATPTTAVSSQSTALPPRPLLQATQLPALVMT
                     GKPGQSSVVYLREAHQRSVSDWIRQLLPPGWQFRQQNAAIPALNKRLPVWSVNDQWHR
                     ALNALLAGQQLYGHMDWNRKILTVTTSATPPVDVTAPQGSQKAADTPRNPFRGSTATP
                     AGPTPVTPAVSVSRAQTTTGTSGTPESRTTTPAPVATKPVVPPQQKVIPPRQWKMEKG
                     TTLKDGLARWTSSEKCPHGTWTLRWETPVNYSIDAPLVFTGSFRQALEQLFALYTPAE
                     KPLYARTSTAQCLLRVTDRPQE"
     gene            12093..12530
                     /gene="pilM"
                     /locus_tag="OQQ48_002363"
     CDS             12093..12530
                     /gene="pilM"
                     /locus_tag="OQQ48_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HCS1953109.1"
                     /translation="MGWVTGFIMVIMAIGNFWFHSHLSRTVHHQQTAEITQQAADFIR
                     YMNAINDYLYQHPERRAAGGQLTSAQLGLPATKNVSHLISQQRVFVWAKEKPGLMGAL
                     LEQSGDSALLARVENGRLLDTRGRRISITLPAVIPDQVIIWMN"
     gene            12562..14181
                     /locus_tag="OQQ48_002364"
     CDS             12562..14181
                     /locus_tag="OQQ48_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN family type IVB pilus formation outer
                     membrane protein"
                     /protein_id="HCS1953110.1"
                     /translation="MNKKLLAIMIAATLLQGCTSFRQAENTRKDAVAEQQRAREMIAE
                     LRNRTPVVKESPRQWINPRPVTDKNPGNAVPGCPIIINTRQSITLQQVAQRISENCHI
                     PVRITPDVQAYLSVTTSGSTQQIQGTVPPPDTGGMVPLAAIGSSDTTLPRLQGNTASL
                     PELKASGLTSLLNAVASRLGISWRYDGSHITFYYLDTRTFPVTYMDSQVAYNATVVSG
                     TMSSNGSSGGTASDAISGDASNTQTTTVDMKSALYQDVKNAVSSMLTPGIGRMFMSTG
                     FITVTDTPQVLETVRTFIEKRNEEMKRQVVLNVEILSIRKTRNEQAGIDWNAVFSDRT
                     LGLSLGSTFTSAASDTVTGGVSIVNGKLTGSKAFLKALSSQGDVSVVTRNSAVTKNLT
                     PVPMQIANQQSYIESVTTDTTANVGSSTSLNAATITTGFNMTLLPFILPDSQTLQLLY
                     SMSLSDKPVIENYESGGSKAQLPNVDLKTINQTVDLKSGQTVIISGFQQSGRRSGKQG
                     VGTPGFFGLGGGINSENDDTILVVLITPNII"
     gene            14202..15497
                     /gene="pilO2"
                     /locus_tag="OQQ48_002365"
     CDS             14202..15497
                     /gene="pilO2"
                     /locus_tag="OQQ48_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4b pilus protein PilO2"
                     /protein_id="HCS1953111.1"
                     /translation="MTDISPTERQVFPLTAGRNVFLAGMDWKTLPASHKNPRTFARSL
                     GAVRYISCEYLSTEDTDRHIMVAAVSQNTLPKGSRRYFSLAMLILPLLEPGGYAIVEL
                     SQANDTELYGFVSAVDGILVSDLVGTREEIREAREIFLTINSAPEHGWTCYEPPSFNG
                     PDGRGPLPLETLTGAGKYPAEARLHPVSRGPQLIPVLLILTLLGTAWYGWQYYERLKA
                     EKAALAEAAQKAAEERITPPWLSMPETGDFIRGCSDVWSRLPVSVAGWRYSLAECAAT
                     GNSGNLRASYTNIAGTTVTAFSQRVREIFSIRPFMVMPDGNSGGFALPVTFSMPETPV
                     AVEALPENTLLQERLTTLARSMQLKMDWQEVSNSFTDEDGNTIQPPWKEYDLQILTTL
                     PAHQVAEHFSEPSVRFISVTRQLEEGRFRYQFTGKYYVQ"
     gene            15487..15945
                     /gene="pilP"
                     /locus_tag="OQQ48_002366"
     CDS             15487..15945
                     /gene="pilP"
                     /locus_tag="OQQ48_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilP"
                     /protein_id="HCS1953112.1"
                     /translation="MSSKTKALLLSVLILPTTVAASTISNMQQNNRQLQVEQIQEETA
                     LYEAMLAREKVRHELRTLEQGEPAEPVHSQESSSAVTTDTPAPITGIPRLTEISGVQP
                     QITARIQLPGGQTADVKEGQHIPGTTFRVEKITTDSVVLEQNGIRTTLQP"
     gene            16049..17557
                     /gene="tadA"
                     /locus_tag="OQQ48_002367"
     CDS             16049..17557
                     /gene="tadA"
                     /locus_tag="OQQ48_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HCS1953113.1"
                     /translation="MLAPADQPPDDILEYVVVDTDKTGQIHVRVVEGKKHLRAVQDYL
                     TRLCSRYPGKVSPFEFTTLDVIARLRQETVISGVENGINPVQQKMLGYISHAATLSAS
                     DLHITPGRDSTDFTYLEARVHGELELLDIVRKDEGLELLGATYSGMTDVIKGTQFDPG
                     VPQDARLAERFLKLAGLFGARYSHYPCVGGLYAVLRLIKDDSQHIPTFSMLGYHPEQE
                     RAVRRMLQRPEGIVILSGPTGSGKSTTLRTASAAYLEQYGFNDTGGILLPRRRLFTIE
                     SPPEGRIPGAIQTAVMDTAQGWVDSVKSALRLDPDAILNGEIRDHASAITAIKAAMTG
                     HLMLTTLHANDPINILERLEMEGVQARMIADPQLFIGLLSQRLVQVICPHCRLPWHEV
                     ESSRTDEERRLVENFCQPDAVYLRNHNGCPHCWRGVNGRTVIAEVISPDAKFFQIYRE
                     KGRIEAKTYWHRELGGMTRNQHLLGKINSGQVDPLAAHYISPVDEDSYTLLH"
     gene            17559..18653
                     /locus_tag="OQQ48_002368"
     CDS             17559..18653
                     /locus_tag="OQQ48_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HCS1953114.1"
                     /translation="MKILERLNNTELELSGLNRWLGKKTFRRTTFYESLAGMIRDGTP
                     VMRALEFICDVETDFGKKKGQSGLYFLATDCIASIRSSGQLSPALKDWVPKDEIALIR
                     NGEERGDIAEAMFQVVKTAKGRQEMISSLVSVCLYPLILLTLCVVNMYNVHTRVIPMI
                     SAFAPEERWSVQMVILAKSSGLVIGYGLWLLAGLLLLAILIRMSFSLYTGPRRRYLDR
                     IPPWSLYQTFHGVNYLFNISSMLQINIPLSHALTRIEKHAEHNRWLKSRISAIRKNVL
                     SGQSLALAMKNCGYDFPSKSCVNNLLLNSEREDSVASMALYADRWLEEAKKNVRKSGI
                     IITAISGLLVFLFIINMVSAIYGISDMLKQ"
     gene            18715..>18921
                     /locus_tag="OQQ48_002369"
     CDS             18715..>18921
                     /locus_tag="OQQ48_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003809694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCS1953115.1"
                     /translation="MSVITSSYSEHNIRHLQNDDKGMTLLEVLGVLVVAAIVIGAVMG
                     LMSDTLSSSDNQKELKNLQTIATKM"
BASE COUNT         4770 a         5075 c         4672 g         4404 t
ORIGIN      
        1 cgaacagcga ccagtacctg gaagagcgtt tgcagctgct ggacgagcag ttggccaccg
       61 tcacccgcct ggccaaggac aacgagctgc ccgatgccat cctcaccgag tcagggctga
      121 aaatcacccc gctggatgcg gcggtgccgg atcgggcgca ggcgctgatc gaccaaacca
      181 gccagttact gccgcgcatc aagatcaccg aactgctgat ggacgtggac gactggacgg
      241 gcttcagccg ccacttcacc cacttgaagg acggggccga ggccaaagac aggacgttgc
      301 tgctgtccgc aatcctcggt gatgcgatca acctcgggct gaccaagatg gccgagtcga
      361 gccccggcct gacctacgcc aagctgtcct ggctgcaagc ctggcacatc cgcgacgaaa
      421 cctattcggc ggccttggcc gagctggtca accaccagta tcgccacgcc tttgccgccc
      481 actggggcga cggcacgacc tcatcctccg atggccagcg cttccgcgcg ggtggccggg
      541 gcgagagcac cgggcacgtc aacccgaagt acggtagcga gccgggacgg ctgttctata
      601 cccatatctc cgaccagtac gcgccgttca gcacccgcgt ggtgaatgtc ggcgtccgcg
      661 attccaccta tgtgctcgac ggcctgctgt accacgagtc cgacctgcgg atcgaggagc
      721 actacaccga cacggccggc ttcaccgatc acgtctttgc cctgatgcac ctgctaggct
      781 tccgcttcgc gccgcgcatc cgcgacctcg gcgaaaccaa gctgtacgtg ccgcagggcg
      841 tgcaagccta cccgacgttg cgcccgctga tcggcggcac cctgaacatc aagcacgtgc
      901 gtgcccactg ggacgacatc ctgcgcctgg ccagctcgat caagcagggc accgtcaccg
      961 cctcgctgat gctgcgcaag ctcggcagct acccgcgcca gaacggactg gccgtggccc
     1021 tgcgcgagct gggccggatc gagcgcacgc tgttcatcct ggactggctg caaagtgttg
     1081 aactgcgccg ccgcgtgcat gccggcctga acaaaggtga ggcgcgcaac tcgctggcca
     1141 gggcggtgtt cttcaaccgc cttggggaaa tcagggatcg gagcttcgag cagcagcgct
     1201 accgggccag cggcctcaac ctggtgacgg cggctatcgt gctgtggaac acggtgtacc
     1261 tggaacgcgc cacccagggg ttggtcgagg ccggcaagcc ggtggacggc gagctgctgc
     1321 aattcctgtc gccgctgggc tgggagcaca tcaacctaac cggcgattac gtctggcggc
     1381 agagccgcag actggaagac gggaagtttc ggcccttacg gatgcccgga aaaccttagc
     1441 gtacgatttt ttccgaattc tgcgggctcc cctccttcat cctctgagga aaacaaatga
     1501 attaactgct tacgcatatg gatacatata cagtattaat ttattaagga gaaaatggca
     1561 tgtcaagatt taatgatagt gattatcatt aacatgaagt aatgttacac cgttcagaaa
     1621 gcctgctgac gctgtggcag catcacagtc tgagtattct ctggtgcaat acaaaaaccg
     1681 gactttcccg taacaacaga caatgttacg ggaaaagtta cagaactgag ccacaaaata
     1741 tctcagtacc tgagatgcat aaaaacaccg tacatatgtt tctcaaaagg gggatgatcg
     1801 ttatccagcc atttcggtgg atcggtatgc agaacaccaa tctgcttcat gccggtatgc
     1861 tgcctggatt ttctcgcgca gtaatcactg tcttcatatc ccatcgtttc gatgaccact
     1921 ctggccgtct ttccgtcagg agcccttgct gtgacaatga aatccggcag gacatatttc
     1981 ttttcaccat taacataaac ttctgtatca aacaggggcc tttccagact taattccggc
     2041 gtacctggct ttcttttaag ccagctggcc acattcagaa gagcagtgag cgtgtttcgt
     2101 tccagtttgc tgtccacagg caccggacat gtcagttgat aaagagcatg agcataccct
     2161 tcactgcaga tgattctgcc atcggcactc cggcaaaaag caagaataac ccagtaaggg
     2221 ggacgtattc cgccctgcgc ttcaccatta atactgatcc cccgcacagg tctgatgacc
     2281 tgaatattac cgttcgccca gtgaatttcc acagcatcac gacagatgtg ctgcgccata
     2341 aaaatctgaa agaacatatg ttttcttctg gcaggccagt gtcgttctga attctccagc
     2401 gtttccatta accgctcctg agacctttca gacattccag gccggaaatg gataatttca
     2461 gataaggatc ttccccgaat atactcctgc tgcagtgtaa cctgccgtat ggcatcaaga
     2521 cactcacgat aattagtccg gatacgggga ttagccaggg aatccagttt attcagccca
     2581 gcatcgtcaa taagagacag gagtaaacgg gcaaggcgcg gacgtttctt gcgaacggag
     2641 gagctctcac cattatggta agcagagcgg gcctgcagtc tgataacaga attactgtca
     2701 tcaggtggta aaaagtttct gtaatttata cgggtgctaa cgggatgccg gcttgctccg
     2761 gaatatgttt cctcttcatt tccggataac tgacgataca tggggcattc aggtgcatgc
     2821 tcatgattaa aactggacag ataaagagta cccgtattgt ttttaagagc agcaaaattc
     2881 atggccggcg aatctcccgg aatacagtca cactgtaacc atgcatcata ctcacgcatt
     2941 tcccggagaa tatcattggc caggttatgt agtgccatgg ttgctggctt tttcagtcca
     3001 cagaccgtct ttactgcagt ctcttcctca ctgctaagct cccgaatcac tttgccccgg
     3061 ggattcttct ttacaagtat cattggcata atgaaagcac tccttatgta catgcttaaa
     3121 cagtaagtaa aatatacaaa gaacacccca tgaaacaaag gcagagatga actgtgcgcc
     3181 ccgatatctt cttctgatat cctttcaaat gaattgtttt tatttatatc atgacaacat
     3241 taaagtgaga gaatatatca accatgtttc tgtcagggtt cagtagaata tcaattcgac
     3301 tgtcacgcca taactgttac tacctcgagt ataaatactc agagaacgga cttattagcc
     3361 atgaaaaatc acctgatggg aaatttacta atttatgaat tgtgatatta ttaaagagta
     3421 aaaccagcca gccgaggtat ttatgggaaa aaatcaggaa gaaaggaaaa ctcccgtcat
     3481 tgtggtgaaa aaacgtcgta ctttttcgcc accgtcctta tccgaaaaaa cagatattat
     3541 cgcacctgta tttactgaac agacggcaga atcagccccc gccggaataa attcatctgc
     3601 cgtcgaaaca catatccccg aagcgccagc caggaagaag aaaaagaaaa gacaccgttt
     3661 tccacgtccc tcccactgga cccgtgagta tactcacgag tgcgtggaaa aaataaaggc
     3721 cctgtttcct catcttcgtg ctgaaggtgg tggatttatt cccctgaaaa ttggcataaa
     3781 caatgatata tcggcatttc tggcagaaca cccggagaca gaactgacca tggacgaatg
     3841 gctctgtgca gtgtcctgca tcacatcccg ccgcgtatat ctgcagcgga cagctgtcgc
     3901 cggcgtccca cgttatggcc tggatggtca ccccaaaggg caggtctcag actctgaagc
     3961 acagagtgcc ggtcgcagac tggcgacatt agaacaaaag tggctcagga tgcaggcaca
     4021 gcaggagaat atttctgggc aataaacact cctgataaca ggtggacatt acgtatcaga
     4081 aaaaatggga agagaatgtt atgtgtgatt cagaagcgga gaagatttta ttcatcctga
     4141 ataaacatga acgggcatac agatgccagt accccgtata acttgataac atcaggaatt
     4201 ttattgataa acagcagtta acgaatagtg atactccgaa gaaaagcacg gtcccaaatg
     4261 aagataaaaa aggcataaga tgatgatcac aggaaatgga ataaacactg taactgtcaa
     4321 tggaaaagtg aaacacatca cagaactgga tgatataaca ctttgtttag agtggacaaa
     4381 actacgagag gaaaacaatc gtctttatga aattaataac caggcaaaca gaggatggcg
     4441 tggtttcatc ctaagattga taggggttaa cctgccagat aaaaggactg agtttacaca
     4501 acgtctctta ttgacgcgca aaatttcggg ttcggtcatg aaaaagtaac atattctatg
     4561 ctggaacaga ccaattttgc attcgccgtc caggagtttg aggggaaagt agtcagtgtt
     4621 tacgctcagt cggggaaagt gtgcaatgat aaacaacacg aaaggaatcg tagtacatgg
     4681 gcaccgcgat tcaatattca aggaaaacac aatacagcaa tgtacttgta ctgtttctat
     4741 tattctggcg atttcaataa aggcacttgc tcgcgctctg tcattttctg aaactcttca
     4801 tgctgcattt cgattttcct tctaagatca taactcgcat acctgttctt tttatgtata
     4861 tctatatcta tttttgtgac gagttcaaac cacaatgttc gcactcccca ggcagcgtta
     4921 tgaaacattt ccgcaacggc tatagagtct gtattaactg cttcggtcat gtagtcgcta
     4981 atacgttcag cggtaatttt cgttatttca tcgtaattca ttttatctcc tacaagatta
     5041 tttaatggaa tgaaaaatat ctggcctgat aataatgttc cagaattaat atctgcaggc
     5101 tatgttaagt tgtccccaca cacaatctac ctgttttctc gtgtgtgcca caggcgcaat
     5161 acataaatag tctggccatt aagttcgtaa cggatttcgt actcgccagc aaaaatccgt
     5221 ctgacctccc tgggttcaaa ctgaaacaac tgttctccca tacgtggatg agttaacaga
     5281 atgaccggtg cctgtgtcag ggattgcacc gttctggcgg ccgcaggttt actggccagc
     5341 accagaaaat catataaccg cgccaaatca gaaagcgcct tactggtcca cttcagttcc
     5401 atcagcgtgg caccggtaac ggattgtcag tactgaggct gtccgcccag gcctgtacag
     5461 cctgatggtc gataacctgt ccggatgtca catcgtccaa ggcttccagc gtcaggcgat
     5521 tacgctcctc ctcctgagca agccatgcag aaagcgcctg tttgataatc cagccccggg
     5581 agcgttccag tctggcggcc atctggtcga ctttatcagc cataggtaat ggcacatgcg
     5641 cggtaattac ttttgttgcc tgtgcggtat tgtttttcat ttgcatatcc gattagctgt
     5701 taatcatatt taatcatagt gattactttc agacgaaaca aggcaaataa tgcgcgtcag
     5761 cgcatgtaag gcggcactcc gttgttacat atggcacaga aacaaaaccg ccgaagcccg
     5821 gcgtcagccg gtactgattg atagatttca ccttacccat ccccagccct gctaagccat
     5881 acccgttttc agccatgaca gagcttctgt gcgcggtcgg agtggtcccg acgagggttt
     5941 acccgaagtc ggggcgtatc tccgcgttag cgggccgaga gggccgctta cgagcgtgta
     6001 ttcagtacgt ccagccagaa gactgacagc gatgatgctg cttagttaca acattcataa
     6061 ttaaaagcga tgttgttccc gccctttggg cgggggcggc ctcgcttttc agttatgagg
     6121 ggtaggcacg gtgctgcgtt tgtgtggtgt ttttattcct gatgtctttt tgctgtggtt
     6181 tcagaacggt gaggttcggg gcccgcgcgt cagcgtggga ggtgcatatc agtctgtcca
     6241 caggacaggc attgaacagg ttttcttttt aaatggaagt aattaagtag tttaaaggaa
     6301 atacagtact ggagtttaaa agaaacattg caccctgtaa cgcctgcggc tggcgcttta
     6361 tagcattaac ggttgtaacc ttatggagaa gtcccttgca gttaaatgtg gataaacaga
     6421 attacccgtc tgtgagacgt gttttgcgtg aaaaacaggg ggagcatgat gagccggaag
     6481 gcagatgatg aggctgtttt ttgtgtgctg tgctgctttt ttgtgcgtcg ccgggcttca
     6541 gacgtgcgga agcctccggc gcaggccgga ttattcagat gaggtcaaac tcagggtgag
     6601 gcagtggcca gccgtgtgta gttgttgttc cgcgacatta tcatgcgttc cttcacgcga
     6661 cggtcgattt cgcgcttcac ggcctccagg cttccgcgga aacgcccttc cgcgatttca
     6721 cgtgtcagct gccgtttcac cagggtgaca atgtcctgac gggtcctgcc tgcatcgcgg
     6781 cgcgcacggg cacgtttcat gccgtgggac ttaagctctg cctgataact gcggaaacgc
     6841 tcgcgcacaa aacgccaggc cctcgctatc agttcatcca tacccagcgc atccatcccc
     6901 tgctttttgc gcagcctgtt ttcccattcc acgcggctgc ggcgtgcgga agccactgcc
     6961 tcttctgaga tatcgagtga gtcaaacagt gccggcgtga acgtgatgtc ggtcgggatg
     7021 ttacagccaa ttgtcgggtc gtattccgtc tggtaggtta tcagccccag ctcagacagg
     7081 aacttcaggg cacgggtggc acgggtgatg gaaagtttcc cggcctcaga ctccgtcgcc
     7141 agtccgcact caatggccag cgtggtgatg gagcactgca cgcggttggc cagcgggtca
     7201 tagtggaaac acagcccctg cagcagcgca tcaatcgccc ggcggcgcag caccggcggc
     7261 atacgccggc gcaggccgcg cgaacgggca tacgccacat gcgcggcaaa atcaaaacgg
     7321 gaggtgaagc ccacggcctt ttccatcagt ttttcgcaga acttcagcgt tccggcatct
     7381 tcacggggtg tgaacaccgg attcgggttc tttacctgac ggtatgtgct ttcagtgaat
     7441 gaggtctggt tcagggccgc cacaagctgt cgcttatggc gggaacagat tgccgtgcgt
     7501 taatgcactg cgaagggttg aatctctgac acggtttcat atatacttat cccgtattct
     7561 gtgaggcccc cattatcttt cagtgattcc gccaagtttc gatgaaagat tatgggggtt
     7621 ttcctttatg gtcttctgaa acttttttca gccttccggc cccggcaaac agccgtcgtt
     7681 tctgccatac atcctgaatg ccttatggcg catcctgccg ttttctgact ttccggatcg
     7741 tgaggatccc aaaagtcact gcataacgat ccgcaatata ccccttttcc accgctttga
     7801 aaagccgaca cgatttctct gatttacctg gcctctcatt aaccacctaa aaagcgtcgt
     7861 ttttttgaac tgtgtcaaaa aaatgaacaa tttcacaaac gatcttttat gctgcttttt
     7921 tcttcttttc cagtagaaga gatcgccgaa tttttttaaa cgatctaata tcccgcacac
     7981 aatatcaaat acagaataat attctgtctt atcagatata acgatctatt caactgctta
     8041 tcttcgctct tgccacagat acatccttat gtaatatatt ttccagccat taatcctgtt
     8101 tgcaggagtt gtcggttttc gcccgcctcg aaaccgtcca caaggcttac aagagtgcgt
     8161 aaatcatgtg cctgtcagaa ctgcaggcca ggctgaacgt gggtcagccg ctgtttccga
     8221 cacatgcttt atgtattcgc cgttcacaac aacatcgaag cccccctgcg gggatcgatt
     8281 ttcgcggagc gttccggatt ttgaatatca gtgaattaca tcaacgcaac tggtatctgg
     8341 cacagtacat ccctaccggc aaaaaccgcg aacatctctt ttcctggctt tccgaacaac
     8401 acgtccttcc ctggacaccc ctgatactga aaaaggtccg ccgcacggat aaagtctgtg
     8461 gttaccgtcg acatatacac gccgtgtttc ccggttactt tttcctgaaa gcagaccccg
     8521 aaagccacag cttcactcac cttcgccgtc acagtgcctt tctggacttc gtgaaaatgg
     8581 ccggtgaaat caaaaccgtc agggaggata tcgttcagtc tttgatgaaa gtgtacccgg
     8641 atcctgcgct caatccggca gccagggagg agctggatgc tgcctccacc ctctggctga
     8701 caaaagcgcg ttaccagtac ctcctccggc ttgatgccca gcctctgccg gagtcgagga
     8761 tcgccctgct cctgcacctg gtatctgatg acggagcgtt gacatgaaaa ttgctgtctt
     8821 catttgacga ctttcccggc ctttatggcc gggctttttt tccccatctt ttccccaatg
     8881 cccggcaccg gtgacacctg cgtgtacgga gagcggctgc cgggcatttt ttttcaggac
     8941 atcgttatga ccattcaatc cccggaaaac cctgtcattc agttactgga gaatatgctt
     9001 gaagcgctct ccacctcccc ggacagcatc aacaatgaac gacgtcgccg gcgcttcctg
     9061 ctgaactggc ttgattccgc ccgacagatg agtgagttca gggggatgcc tgaagagttc
     9121 accgccctgc gcaaactgct ggccaccaat acggacatac cggtagagaa cacgctgaaa
     9181 tcactgctgg aaagtggcca caacgcagca ctgtatgacc tgtaccgctt ccgagcagcg
     9241 cttgccacgt gcaggagtct gggatggcag attggcacgt gcgcatggaa tgaccagtta
     9301 ctgagtgaga cactttcccg cgcacagacc gggaaacgtc atatcctgca gctgaacagc
     9361 accggggaca ccttttccac cacgggcaga atgatcagac cggtggcatt ttcattaatc
     9421 catccggcca ccatcaacac gtctgaggtt gaagagatat tccgggatga aggttttatg
     9481 ctcgcacacg ggcgcgaatg cagtctgaac ggcagcggca gaaatatgct gtcacgcatt
     9541 cttcatgtgg gccattcagg tttagcggaa gaggaatggg gcatggacaa cagtctgctt
     9601 ttacactgaa ataccgggac agccacaacg gcagccactc tccgctgact ttttttcgca
     9661 aatcgcggtt tgctgatgtg gatgtcccat ttttgattat ccgtcctctc cggttgtcat
     9721 aaggcgcatt ccggcgtgag gcttctgttg ttatcatccc ctcctgcaga catccccttg
     9781 tcttctgatg actgacgata tgcttcttct gacaattaaa gaagcggaag cttcacgcca
     9841 taatgttgtt tgactgacct gccacaggag gaaaaatgac actgacgtta cttaccgcgt
     9901 ataacgcacc ctatctggca gccctgacac gtccactggt catgcctgcc ccggaaaagg
     9961 ttccggatgt cagcgaaccg gtaatcgctc cggataactc agaaccacaa caggtttaat
    10021 ccccgtgccg gaatttttcc ggcatctttt tcacaaagaa tgccctcacg ggatattctg
    10081 gtttaaagga ggattgaaaa tgtccgcaga aaaattcaga gttgtcatat acaccaatac
    10141 atgtgaaaag aaagtcagtt atatttctgc aacagaagat ataaacatcg ctataatgcc
    10201 atttcagacg gcagacaatg gcttaattat tgtgtgtcag aactcccgta taatacgacg
    10261 caaagagcgt atccccggaa gcagctgtga atggaaagat gtgacattag caccaaaatc
    10321 cagaaaccgc gttcagttat agcctgacag cctgcttctc atctcccata aaaggggata
    10381 aaaattaaat atttcacaac cgcgatattt ttcaggaatg cagattattt tctgttatga
    10441 ccaatggaga aacaggcatg agaacaatca caacacgtga acaattatta attaatggga
    10501 gagtccacga aagaatcgcc acacatatag ttacaggagc tcatgggtat gaaacgcttt
    10561 gcacaaatgg ctataacgtc agatacaaca aggcgcagca actggttgaa agctgcgaaa
    10621 aaatagctga aggtaaacta cctgttacct gtcctgtctg tttcaccata tggcaggatg
    10681 tacacagatt cacccacgtt gactttgaca cacaatcggg gaaaagtgat tttattgata
    10741 cctgccattc ggagatcata accggcatgt ttcaataaca ggcaagaaat aaaataatgt
    10801 tcaagttctg aaaaacaata tcacttgttt tctggaaact atcatttgca gcaggacgtt
    10861 tttcttttta tatttccgga accggaatgt tttagttttc ataatcaccc cgaacatgaa
    10921 tgttcatcat caatatcctg atgaaattat atttatctga agacctgtta ccagccacct
    10981 gtcttatacc gtaacccgat acataccgga tttattctat gaaaaagaaa tcacttacac
    11041 tccttctgcc gtcattactc agcggctgta cggcatttaa tgccacgcct gttcctgaca
    11101 ccacgcctcc ggctctggtg ttcgtcgacg gacaaattcg cgaaagcgct gccattatcg
    11161 cccatacgca aaaacgtctg cttccgccac cggtgacccg ccctgtgacg ccgccccgcc
    11221 cgacaacaac agcgacgccc acaaccgcag tcagcagcca gagtaccgca ctgcctcccc
    11281 gtccgctcct tcaggccaca cagctgcccg cactggttat gaccggtaaa cccggtcagt
    11341 catccgtcgt ttatttacgt gaggcacatc agcgctccgt cagtgactgg atacgccagt
    11401 tgctaccacc cggctggcag ttccggcaac agaacgctgc cataccggca ctgaacaaac
    11461 gcctccccgt ctggtccgtt aatgaccagt ggcaccgggc actgaatgcg ctgctggccg
    11521 gccagcagct ttacggacat atggactgga acaggaaaat actgactgtc acgacctctg
    11581 caacaccgcc ggtcgacgtc acggcaccgc agggttcaca gaaagctgct gatacaccgc
    11641 gtaatccgtt caggggcagc accgcaacgc cggcaggccc gacaccagtg acacctgccg
    11701 tcagcgtttc ccgggctcag accaccaccg ggacatccgg aactccagag agcagaacaa
    11761 ccacgccagc cccggtggcc acgaaaccgg tagtgccacc acagcagaag gtcataccgc
    11821 cacggcagtg gaaaatggaa aaggggacca ccctgaaaga cggactggcc cgctggacat
    11881 catccgaaaa atgcccgcac ggcacatgga cactgcgctg ggaaacacct gtcaactaca
    11941 gcatcgatgc ccctctggtt ttcaccggca gtttccgcca ggcgctggag cagctctttg
    12001 cactctatac cccggctgaa aaaccgctgt atgcccggac cagcactgca caatgcctgc
    12061 tccgtgtgac agaccgcccg caggaataac ctatgggatg ggttacgggt ttcattatgg
    12121 tcattatggc cattggtaat ttctggttcc acagtcacct cagccgtact gtgcaccatc
    12181 agcagaccgc tgaaatcaca cagcaggctg cagactttat tcgttatatg aatgccatca
    12241 acgattacct gtatcagcac cctgaacgac gtgctgccgg tggtcagctc acgtcagcac
    12301 agctcggtct gcccgccacg aaaaacgtca gccacctcat cagtcagcag cgcgtttttg
    12361 tctgggcaaa agaaaaaccc ggccttatgg gtgccctcct ggaacaaagt ggtgattcgg
    12421 ccctgcttgc cagagtggaa aacggtcgcc tcctggacac ccgtgggcgg cgtatttcca
    12481 tcacgctccc tgctgtgatc cccgatcagg tcatcatctg gatgaattag gccggactct
    12541 gaataactca cggatttatc tatgaacaaa aaacttcttg ctattatgat tgcagccact
    12601 ctgctgcagg gctgtaccag tttccgacag gcagagaaca ccagaaaaga cgccgttgct
    12661 gaacaacaac gggcccggga aatgatcgct gagctgcgaa accggacacc ggttgtaaag
    12721 gaatccccac gtcagtggat caatccccgt ccggtcacgg ataaaaaccc cggtaatgcg
    12781 gtgcccggct gtccgattat cattaacacc cggcagagca tcacccttca gcaggtggcc
    12841 cagcggatct cggaaaactg tcatattcct gtaaggatca cccctgatgt acaggcgtat
    12901 ctgagcgtga ccacctccgg cagtacgcag caaatccagg gcacagtacc accgccggat
    12961 accggcggta tggttccact ggccgccata ggcagttctg acacgacact tccccgcctg
    13021 cagggaaata cggcgtcact tcctgaactg aaggcttcag gactgacatc actgctgaat
    13081 gcggtcgctt cccgtctcgg gatttcctgg cgttatgatg gttcacatat cacgttctat
    13141 tatcttgata cccggacctt cccggtgacc tatatggaca gccaggttgc ctataatgcc
    13201 accgtggtca gcggtaccat gtcatcaaat ggctcatcag gtggaacagc atcggatgcc
    13261 atttccggtg atgccagtaa tacccagacc accaccgtgg atatgaaatc tgcgctgtat
    13321 caggatgtga aaaatgctgt ctcatcaatg ctgacgccgg gtattggtcg catgttcatg
    13381 tcgacgggct ttatcaccgt cacggacacc ccacaggtac tggaaacggt ccggacgttc
    13441 attgaaaaac gcaatgaaga aatgaagcgc caggtggtgc tgaatgtgga aattctgagt
    13501 atccggaaaa cccgtaatga gcaggcaggt atcgactgga acgcagtctt cagcgacaga
    13561 acgctgggac tgagcctcgg cagcacattc accagtgcag ccagcgatac cgtcactggt
    13621 ggtgtttcca ttgtgaacgg gaaactcacc ggttcaaaag ccttcctgaa agcactctct
    13681 tcccagggag atgtcagtgt ggtaacccgg aattccgccg tgaccaaaaa cctgaccccg
    13741 gtaccgatgc agattgccaa tcagcagagc tacattgaga gtgtgacgac ggataccacc
    13801 gccaatgtcg gctcatccac ctccctgaat gcggccacca tcaccaccgg ttttaatatg
    13861 acccttctgc cgttcattct cccggactca cagacactgc agctgctcta ttccatgagt
    13921 ctctccgaca aaccggttat tgagaattac gagagtggtg gaagcaaagc ccagctgccg
    13981 aatgtggatc tgaaaaccat caaccagaca gtggacctga aatccgggca gacagtcatt
    14041 atcagtggct tccagcagtc agggcgtcgc agtggaaaac agggtgtcgg cacaccggga
    14101 ttcttcggtc ttggcggtgg gatcaacagt gaaaacgacg acaccattct cgtggtgctg
    14161 atcacgccca atatcatctg atttgcagaa ggaggactat cgtgacggat atttcaccga
    14221 cagagcgaca ggtgtttccg ctgacggcag gacggaatgt atttcttgcc ggaatggact
    14281 ggaaaacact gcccgcaagc cataaaaacc cccggacgtt tgcccgcagt ctgggggcag
    14341 tacgctatat ttcctgtgag tatctcagta cggaagatac cgatcgtcac atcatggttg
    14401 cagccgtcag ccagaataca ctccccaaag gcagccgcag gtatttctct ctcgccatgc
    14461 ttatcctgcc cctgctcgag ccaggaggat acgctatcgt tgaactgtca caggcaaacg
    14521 atacagaact ttatggtttt gtttctgctg ttgacggcat cctggtcagt gacctggtgg
    14581 gaacccggga ggaaatcagg gaggcccggg aaatattcct caccattaac agcgcgcctg
    14641 aacacggctg gacatgttat gaaccgccct cgtttaacgg gcctgacggc agaggtcccc
    14701 tgccactgga aaccctgaca ggtgcaggga aatatccggc agaagcacgc cttcaccccg
    14761 tatcccgtgg gccacagctt attccggtat tactcatact cacccttctg ggtactgcat
    14821 ggtatggctg gcagtactat gaacgcctga aggcagaaaa agcagcactt gctgaagcgg
    14881 cacaaaaagc cgcagaggaa cggatcacac caccgtggct gagtatgccg gaaaccggag
    14941 attttatccg gggatgcagt gacgtatggt cacgcctgcc ggtgtcggtc gccggctggc
    15001 gatattcact cgccgagtgt gctgccaccg ggaacagcgg caatctgcgt gcatcatata
    15061 caaacatcgc cgggacgaca gtgaccgcat tcagccagcg ggtcagggaa attttcagca
    15121 tccggccgtt tatggttatg ccggacggga atagcggtgg ttttgccctg ccggtcacct
    15181 tcagtatgcc tgaaactccc gttgccgttg aggcattacc ggaaaacacc ctcctgcagg
    15241 aacggctgac cacactcgcc cggtcgatgc agctgaaaat ggactggcag gaagtcagca
    15301 acagcttcac cgatgaagac ggaaatacca tccagccccc ctggaaggag tatgacctgc
    15361 agatactgac gacacttccg gcgcaccagg tggcagagca tttcagtgaa ccctccgtcc
    15421 gttttatctc cgtaacaagg caactggaag agggtcgttt ccgttatcaa ttcacaggta
    15481 aatattatgt ccagtaaaac aaaagctctt ctgctttccg tcctgatcct gccgacaacg
    15541 gtcgcggcca gcaccatcag caacatgcaa cagaataacc ggcaactcca ggtggaacag
    15601 attcaggaag aaactgcact gtatgaagcc atgctggcaa gagaaaaggt gcgacatgaa
    15661 ctcagaaccc tggagcaggg agaaccagct gagcctgtgc attcacagga aagcagttca
    15721 gccgtcacga cagatacccc tgcccccatt accggtattc cccggctgac agagatatcc
    15781 ggagttcagc cccagataac cgccaggata caactgccgg gcggacaaac tgctgatgta
    15841 aaagaagggc aacacattcc cggcaccaca ttccgcgtgg aaaagatcac cactgacagt
    15901 gttgtccttg aacaaaatgg tatcagaaca acactacaac cctgactcag tgccgccatt
    15961 tacagccggc tgtcggaaca gattattccc ccggccctta atcacaaaaa aaatcgtttc
    16021 cgggtaccgc tgcccgtaag gagattttat gctggcacct gcagaccagc cacctgatga
    16081 cattctggaa tacgtggttg ttgatacaga taaaacagga caaattcatg tcagagtggt
    16141 ggaaggaaaa aaacaccttc gtgccgtcca ggattatctg acccgcctgt gctcccgcta
    16201 tccgggtaaa gtatccccgt ttgagttcac cacacttgat gtgatcgccc gactcaggca
    16261 ggagacggtc atttccgggg tggaaaacgg aattaacccc gtacagcaaa aaatgctcgg
    16321 gtatatcagc catgcggcca ccctcagcgc ctcagatctt cacatcacgc cggggcgtga
    16381 cagtacggat ttcacgtatc tggaggcccg tgtccacgga gagctggagc tgctcgatat
    16441 cgtcagaaaa gatgaggggc tggaattact gggggccacc tattccggaa tgactgacgt
    16501 cattaaggga acacagtttg atcccggtgt cccccaggat gcccgtctgg ctgaaagatt
    16561 cctgaaactg gccggactgt ttggtgcgcg ctactcccac tatccgtgtg tgggcggact
    16621 ctatgccgta ctgcgtctga tcaaggatga cagtcagcat ataccgacat tcagtatgct
    16681 ggggtatcac cctgagcagg agcgcgctgt ccgaaggatg ctgcagcgac cggagggcat
    16741 cgtgatcctc tctggcccca ccggttccgg aaaatccacc acgctgagga cggcttctgc
    16801 cgcctatctg gaacagtatg gcttcaacga taccggaggg atcctgctcc cccggcgacg
    16861 tctgttcaca atagaatcac caccagaggg gcgtatcccc ggtgcaattc agacggccgt
    16921 tatggatacc gctcagggct gggtcgattc tgttaagtcc gccctgcgcc ttgatcctga
    16981 cgctatcctg aacggggaga tacgtgacca tgcctccgcc atcacggcga taaaagccgc
    17041 catgaccggt cacctgatgc tgaccaccct tcatgctaac gacccgataa atattcttga
    17101 gcgtctggaa atggagggcg ttcaggcaag gatgattgca gatccgcagt tatttatcgg
    17161 cctgctcagc cagcgtctgg ttcaggtgat atgtccccac tgccgcctcc cctggcatga
    17221 ggtggagagc tcccggacgg atgaagaacg acgcctggtg gaaaacttct gccagcccga
    17281 tgccgtgtat cttcgtaacc ataacggctg cccacactgc tggcgtggag tgaacggccg
    17341 gaccgtcatt gcagaagtga tttccccgga tgcgaagttt ttccagattt accgggaaaa
    17401 aggccggatt gaggcaaaaa cctactggca ccgggagctc ggcggtatga cacgaaatca
    17461 gcacctgctg ggcaaaatca acagtggtca ggtcgatccg ctggctgcgc attacatatc
    17521 ccctgtagac gaggacagct atacgctgct gcactgatat gaaaattctg gaaagactca
    17581 acaatacgga actggaactg tccggcctta accgctggct gggcaaaaag acgttcaggc
    17641 ggactacgtt ttacgagtcc cttgcaggca tgatccggga cggcacaccg gttatgcggg
    17701 cactggagtt catctgcgat gtggaaacgg attttggtaa aaagaaaggg cagtccggcc
    17761 tgtattttct ggcgacagac tgtatcgcca gcatacgcag cagtggtcag ctctctccgg
    17821 ccctgaagga ctgggtaccc aaagatgaaa ttgccctgat ccgtaacggt gaagaacgag
    17881 gtgatatcgc tgaagccatg tttcaggtcg tcaaaacggc aaaaggacga caggagatga
    17941 ttagcagtct ggtgagtgtc tgcctctatc cgctcatcct gctgacactc tgtgtggtga
    18001 atatgtataa cgttcatacc cgggttattc cgatgatcag cgcctttgca ccggaagaac
    18061 gctggtcagt acagatggtc attctggcca aatcctccgg actggttatc ggttatggtt
    18121 tgtggctgct ggcaggactg ctgctgctgg caattctcat caggatgtca ttcagtctgt
    18181 ataccggtcc ccgacgacgg tatcttgatc gcatcccgcc atggtccctg tatcagactt
    18241 tccatggcgt gaattacctt tttaatattt catccatgct gcagataaat attcctttat
    18301 ctcatgccct taccagaata gaaaaacatg cagaacataa ccgatggtta aaatcacgaa
    18361 taagtgctat cagaaaaaat gtcttatccg gtcagtcact ggcactggcc atgaaaaact
    18421 gtggttatga ctttccgtca aaatcctgtg tgaataatct tcttttaaat tcagaaaggg
    18481 aggacagtgt tgccagtatg gcgctttatg cagaccgctg gctggaagaa gcaaaaaaga
    18541 atgtcagaaa aagcggcatt attattactg ctatatcagg tctgctggta tttctcttca
    18601 tcatcaatat ggtcagtgcc atttacggca tcagtgacat gctgaagcag taaacacagc
    18661 atcctttata ttcccctcga tccaaattct gttttaccaa tctcaggagc taatatgagc
    18721 gtcataacct cttcgtattc agaacataat attcgtcacc ttcagaacga tgataaaggc
    18781 atgaccctgc tggaagttct cggtgttctg gtcgtcgctg ccattgttat tggtgccgtg
    18841 atgggactga tgtccgatac cctgtcctct tcagacaacc agaaggaact caaaaacctg
    18901 cagacaatcg ccaccaaaat g
//
LOCUS       DAJVUJ010000077        18733 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.077, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000077 DAJVUJ010000000
VERSION     DAJVUJ010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18733
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.077"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(31..2478)
                     /gene="glgP"
                     /locus_tag="OQQ48_002370"
     CDS             complement(31..2478)
                     /gene="glgP"
                     /locus_tag="OQQ48_002370"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HCS1953116.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2497..3930)
                     /gene="glgA"
                     /locus_tag="OQQ48_002371"
     CDS             complement(2497..3930)
                     /gene="glgA"
                     /locus_tag="OQQ48_002371"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HCS1953117.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRASLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3930..5225)
                     /gene="glgC"
                     /locus_tag="OQQ48_002372"
     CDS             complement(3930..5225)
                     /gene="glgC"
                     /locus_tag="OQQ48_002372"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HCS1953118.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLKAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5243..7216)
                     /gene="glgX"
                     /locus_tag="OQQ48_002373"
     CDS             complement(5243..7216)
                     /gene="glgX"
                     /locus_tag="OQQ48_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HCS1953119.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWDSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(7213..9399)
                     /gene="glgB"
                     /locus_tag="OQQ48_002374"
     CDS             complement(7213..9399)
                     /gene="glgB"
                     /locus_tag="OQQ48_002374"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HCS1953120.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDASISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9672..10775)
                     /gene="asd"
                     /locus_tag="OQQ48_002375"
     CDS             complement(9672..10775)
                     /gene="asd"
                     /locus_tag="OQQ48_002375"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCS1953121.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10967..11560
                     /gene="yhgN"
                     /locus_tag="OQQ48_002376"
     CDS             10967..11560
                     /gene="yhgN"
                     /locus_tag="OQQ48_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HCS1953122.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11617..12957)
                     /gene="gntU"
                     /locus_tag="OQQ48_002377"
     CDS             complement(11617..12957)
                     /gene="gntU"
                     /locus_tag="OQQ48_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCS1953123.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGPLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(12961..13488)
                     /gene="gntK"
                     /locus_tag="OQQ48_002378"
     CDS             complement(12961..13488)
                     /gene="gntK"
                     /locus_tag="OQQ48_002378"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCS1953124.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMLRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13627..14622)
                     /gene="gntR"
                     /locus_tag="OQQ48_002379"
     CDS             complement(13627..14622)
                     /gene="gntR"
                     /locus_tag="OQQ48_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HCS1953125.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHCHIAYLGARLDERTIIKQKGYEQAMLDAGLVSYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14846..15541)
                     /gene="yhhW"
                     /locus_tag="OQQ48_002380"
     CDS             complement(14846..15541)
                     /gene="yhhW"
                     /locus_tag="OQQ48_002380"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HCS1953126.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15664..16701)
                     /locus_tag="OQQ48_002381"
     CDS             complement(15664..16701)
                     /locus_tag="OQQ48_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCS1953127.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFISDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLSVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     PEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            17033..17521
                     /gene="yhhY"
                     /locus_tag="OQQ48_002382"
     CDS             17033..17521
                     /gene="yhhY"
                     /locus_tag="OQQ48_002382"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCS1953128.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            17758..>18733
                     /locus_tag="OQQ48_002383"
     CDS             17758..>18733
                     /locus_tag="OQQ48_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HCS1953129.1"
                     /translation="MSNIVYLTVTGEQQGSISAGCGTSESTGNRWQSGHEDEIFTFSL
                     LNNINNTGLGSQFHGITFCKLIDKSTPLFINSINNNEQLFMGFDFYRINRFGRLEKYY
                     YIQLRGAFLSAIHHQIIENQLDTETITISYEFILCQHLIANTEFSYLALPENYNRLFL
                     PNSKNQTNNRFKTLNSKAIGRLLAAGGVYNGNIEGFRDTAEKLGGDAIKGYDQILNEK
                     TAGIAIATASILLTKRSNVDTYTEINSYLGKLRGQQKLLDGIDIIEIIYIKRPSKDLA
                     NLRKEFNKTVRKNFLIKLAKTSEASGRFNAEDLLRMRKGNVPLNYNVHH"
BASE COUNT         4764 a         4978 c         4574 g         4417 t
ORIGIN      
        1 cctttggccc cgttctattt attggtgaac ttacaatctc accggatcga tatgccagat
       61 atgatcggcg tactctttga tagtacggtc agaagagaag tagcccatat tggcaatgtt
      121 cagcatcgct tttgcggtcc actcttcctg aagctcgtag agttcgtcga ctttatcctg
      181 acaatcgaca tagctgcgat aatccgccag aacctgatag tgatcgccga agttgatcag
      241 cgaatcaacc agatcgcgat agcgacccgg atcttccgga ctgaatacac cgctgccgat
      301 ttgcgtcagc acctgatgca gctcctcatc tttctcgtag tattcacgcg gtttgtagcc
      361 ctgacgacgc agttcttcca cttcttccgc tgtgttacca aaaataaaga tattgtcagc
      421 accgacatga tccagcatct cgacattcgc accgtccaac gtaccgatag tcagcgcacc
      481 gttaagcgca aacttcatgt tactggtgcc ggaagcttcc gtccctgcca gcgaaatctg
      541 ttcagacaga tctgccgccg gaatgatcaa ctgcgccagg ctaacgctgt agttcgggat
      601 gaacacgact ttcagcttat cgccaatctg cggatcgttg ttgatcactt tcgctacgtc
      661 attgatcaaa tgaataatgt gcttcgccat gtaataggcc gaagccgcct taccgccaaa
      721 aatattcacg cgcggtaccc acttcgcatc cgggtcggcc ttgatgcggt tatagcgggt
      781 aatcacatgc aacacattca tcaattgacg tttgtattcg tgaatacgtt tgatttgtac
      841 atcgaacaac gcctttggat tcaccaccac attcagctgc tgggcgatat actctgccag
      901 acgctttttg ttctccagct tcgcctgatg cacagcgtga ttaaccattg ggaaatcaca
      961 gtgttgttgc agctcattaa gcaggctaag gtcggtgcgc cagttacggc ccaggtgttc
     1021 gtccagcacg gctgaaagcg atgggttcgc taccgctagc cagcgacgcg gcgtcacacc
     1081 gttggtgacg ttggtgaaac gacccgggaa gattttcgca aagtcggcaa acaacgattg
     1141 caccatcaga ttagagtgca gttccgatac accgttaact ttgtggctca caacaaccgc
     1201 cagccaggcc atacgcacac gacgaccgtt ggattcatca atgatcgacg cccgtcccag
     1261 cagatcggta tcgttcggat actgttcctg caaggttttc aggaaatagt cgttgatttc
     1321 aaagatgatc tgcaggtgac gcggcagaat tttacccagc atatcaaccg gccaggtttc
     1381 cagcgcctcg ctcatcagcg tgtggttagt gtaggagaag acctgacaac acacctcaaa
     1441 cgcgtcgtcc cagctaaatt ggtgctcatc gatcagcaga cgcatcatct caggaatcga
     1501 cagtaccgga tgggtatcat tgagatgaat cgcgatttta tccgccaggt tatcgtaggt
     1561 tttatgcaac tgataatggc ggcttaaaat gtcctgaatg gtcgaggaaa ccagaaagta
     1621 ttcctgacgc aggcgcagct cacgcccgga gtaggtggag tcatccggat acagtacgcg
     1681 agatacgttc tcggagtggt ttttatcttc cactgccgcg aagtagtcac cttggttgaa
     1741 tttaccgagg ttaatttcgc tactggcttg cgcactccac aaacgcaacg tgttggtcgc
     1801 gtcggtgtcg taaccaggga ttatctgatc gtaagcgact cccagaatct cttcggtttc
     1861 aatccagcgc gtttttttac cttcctgctg aatgcgacca ccaaaacgga ctttatagcg
     1921 cgtgttgtgg cgtttgaatt cccacgggtt accgtattcc agccagtagt ctggcgactc
     1981 tttctggcta ccgttaacga tgttctgctt gaacataccg tagtcatagc ggatgccgta
     2041 accgcgcccc ggcaacccta acgtcgccag agaatcaagg aagcaagccg ccagacgtcc
     2101 caggccaccg ttaccgaggc ctgggtcatt ttcttcatca atcagctctt cgagatttaa
     2161 ccccatcgct tccagtgcgc cctgtacatc ttcgtaaatt cctagcgaca acatggcgtt
     2221 ggagagcgta cggccaatca aaaactccat cgacaggtag taaacctgac gagtttcttg
     2281 cgacaactgg gcacggtttg aacgtaacca gcgctccacg agacgatcgc gcacagcaaa
     2341 taacgttgcg ttcagccatt catgtttatt ggcgacgacc gggtcctttc caatcgtaaa
     2401 catcagcttg taagcgatag agtgcttaag agcttctacg ctaagcgtgg gcgatgaata
     2461 tgtaaacgga gcattcatat aggcgtttcc tgaaaactat ttcaagcgat agtaaagctc
     2521 acggtacgac ttcgccgcga cctgccagct aaaatccatt gccatagcct gacgttgcac
     2581 aaaccgccac agtgaagcac gagaccacag tacaaaagca cgtcgaatag cccgtaacag
     2641 cgaccaggca ttactatctt caaagacaaa cccactggcg acgccatctg caaggttctc
     2701 aagagaacag tcagaaaccg tatcagcaag cccaccggtg cgccgcacta acggcagcgt
     2761 accgtacttc aatccataaa gttgcgttaa gccgcacggt tcaaaacggc tgggcaccag
     2821 aatgacgtcc gcgccgccca taatgcgatg cgaaaatgct tcgtgatagc caatctgaac
     2881 gcccacctgg ccggggtatt ccgctgccgc cgcaaggaaa ccttcctgca gcaccggatc
     2941 gcccgcgccg agtagcgcca gctgcccgcc ctgctccaga agacccggta aggcttccag
     3001 caccaggtcg agacctttct ggctggtcag acggctcacc actgcaaaaa gcggcacttt
     3061 atcgtcaacc ttaagcccca ttgcgatttg taactggcgc ttattttccg ctttatcttc
     3121 caacgtatcg cgggtgtaac gcgaggccaa cagtaagtcc gtctctggac tccagatttt
     3181 ctcgtccacg ccgttcagta cgccggaaag acgcccttca cggtgacgct gttgcaacag
     3241 accttccata ccgtaggcaa actgcggttc ggtgatctcg cgagcgtagg ttggactgac
     3301 cgccgtaatg tgatcggcat agtacagacc ggccttcagg aaagagattt gtccgttgaa
     3361 ttccagccca tgaatattaa agaatgacca tggcaattgg atgtcattca tgtgatgtgc
     3421 ataaaacatg ccttgatagg ccaagttgtg cacagtaaac accgacttcg ccggacgccc
     3481 gcgcgccgcc agatacgcag gcgcaaggcc tgcatgccag tcgtgcgcat gcaccacatc
     3541 aggacgccag aatgggtcaa gcccgctggc catttctgcc ccaacccacc ccagcagcgc
     3601 aaaacgcaat acgttgtcgg tataggcaaa taagttggta tcgtgatacg ggcttcccgg
     3661 acgatcatag agatgcggcg cgtcaatcag gtatatgcca accccgttgt aatgaccgaa
     3721 caacagcgtg atatgtccgg cgaaggtatc acgacgggat actacctgcg catcggtcac
     3781 gccacggcga atatcgggaa atgcaggcaa cagtacgcga gcgtcaacgc cgtctgcgat
     3841 ttgtgctgcg ggtaatgccc caataacatc agccagaccg ccggttttaa gcagcgggaa
     3901 catctctgaa catacatgta aaacttgcat tatcgctcct gtttatgccc taacttccgt
     3961 agcatttcgc gcgttaccag cacgatgcct tcttctgaac gatagaaacg acgtgcatct
     4021 tcctctgcgt tttcaccaat caccatgcct tccgggataa cacaagcacg atcgatgacg
     4081 cagcggcgca gacggcacga gcgacctacc catacttccg gtaacaatac ggcggaatca
     4141 atgttgcaga atgaattcac gcgaacgcgc gagaacagaa cggactgcac caccaccgaa
     4201 ccggagatca cacaaccgcc ggataccagt gagttaaggg tcatcccgtg gctaccggag
     4261 cgatcctgca cgaatttcgc tggcggtaat gattcattgt aggtgcgaat tggccaattg
     4321 cgatcgtaca tatccagttc cggcaccaca gaggccagat cgagattcgc tttccagtaa
     4381 gctttcagcg tacccacatc gcgccagtac ggctcggcat ccgggtcgga ttgtacgcaa
     4441 gagagcggga acgggtgcgc ataggccaga ccggcttcgg tgatcttggg aatcaaatct
     4501 ttgccaaagt cgtggctgga gttctcatca cgatcgtctt cttccagcag ttcatacaga
     4561 tagtcggcgt caaagacgta gatacccata ctcgccagag atttgctcgg atcgttcggc
     4621 attgacggtg ggttagcagg tttttcaacg aattcgataa ttttatcgtt ctcatcaacc
     4681 gccataacgc caaatgcgga ggcttcttca atcggtactg gcatacaagc aacggtgcaa
     4741 cgtgcgcctt tttcgacgtg atcgataagc atacgcgagt agtcttgctt gtagatatgg
     4801 tcgcccgcca ggatcaccac gtattccgct ttataacggc ggataatgtc gaggttttgg
     4861 gtgaccgcat ctgcggtgcc gcgataccag ttttcccctt tcattctctg ctgtgctggc
     4921 agcagatcga caaactcgtt catttcttca ttgaagaatg accagccgcg ctgaatgtgc
     4981 tgcaccagag tgtgggactg gtactgggtg atcacgccca tacgacggat cccggagttg
     5041 atgcagttag acagcgcaaa gtcgataatg cggaacttac cgccgaagtg tacggccggt
     5101 tttgctcgct tattggttaa atccttcagg cgggtaccac gtcctcccgc cagtatcagg
     5161 gcaacagatt tcaatggcag ctggcgcgcc aacattaagt gatcgttctt ctctaaacta
     5221 accatgacta actccttttt tatcatctct ggaacacaca caatccgtgt gcaggtccct
     5281 gccagacagc cgtaatcact gggttatcct ctccagcgaa tgggggaatg gcgtgccact
     5341 ccccagcagg taaaacaatc tctgttacct caagcgtggc gttaattgcg atcaaaaagc
     5401 gatccgagag cagaatttgc agctgtttcg gcccgttttg ccactcatcc gtgcttaaag
     5461 gttgagcata tcgatttagc caacggacat tgccatcgcc ttcttcccac cagcgattct
     5521 ccaccaaagc gggaatgcgc ttgcgcagat ggattaacgc ggcggtaaat gcggttaaac
     5581 cactgcttgc ctgcgaccag tccaaccagg ttaattggtt atcctgacag taggcattgt
     5641 tattgccatg ctggctgtga ccatgttcgt caccggccag taacatcggc gtaccctggg
     5701 agagcaacaa cgttgttaac aaggcgtgaa tactgtcgcg ccgccgttca accaggtcaa
     5761 gagaaccgcc taacccttct ttaccatgat tgttactgta attgttgttg gtcccgtcgc
     5821 gattttcttc tccgtttgct tcattgtgtt tatggttgaa gcaaacgcag tcgcgaagcg
     5881 taaaaccgtc atgcgcagtg acgagattaa tcgcggcact cggcagacga ccattacgtt
     5941 taaaaacatc gctggaggca gcaaaacgcc cggcaaacgc ccccagaggc aaatcataat
     6001 gtagccagaa acgacgggca gcatcgcgga aatgatcgtt ccactcggca aacagcggcg
     6061 ggaaatttcc cacctgataa ccaccagggg cgatatccca cggttcagca attaacttca
     6121 cctgcgagag caccgggcag ttctggatag cggtaaacaa cggcgcatcc tgacggaact
     6181 ctggcgtacg gcccatgact gccgccagat caaagcggaa accatcgacg tgacaggttt
     6241 ctacccaata acgcaggcag gcgctggcat aatccaccac cgccggatga ctcaaattga
     6301 gcgtgttgcc gcaaccggtc cagttgtgat aatcgccgtc ttctcttatc caataatagc
     6361 tacggttatc gatcccacgc agtgagaata acgggccgtc gaggtccagt tccgcactat
     6421 ggttgagcac gatatcaaga atgacttcga tacccgcttt atgcagtgct ttgattgcat
     6481 cgcgaaactc atccagcgcc gtttctggcg agcaggcata cgccggatgc agcgcaaaca
     6541 tcgccaccgg gttgtaaccc cagtagttac ttagccccat gcgttgcaga cgtggttcac
     6601 tggcaaactg cgccactggc agcagttcca gcgcggtaat gcccaattgt ttcaaatagt
     6661 tgatcatcac cggatgcccg agggctttat aagtgccacg gatctcgacc ggaatctccg
     6721 ggtgcaagta cgttaatcct ttgacatggg cttcataaat gatggtgctg tcccacggcg
     6781 tgcgcggcgg ggcatcatct tcccagtcat agtgatcaac caccactacg catttcggcg
     6841 caatggcggc gttgtcgcga tagtcaggtt cattatgacc ggcgtgcagc agcgggttat
     6901 ctttaaattc cccgtcaatt tgccgcgcgc aaggatcaat caacaacttc gccgggttaa
     6961 agcgatgccc ctcggcgggt tgccaggggc catgaacgcg ataaccataa cgcaaacccg
     7021 ggcgcgcatc cggcagataa ccgtgccaaa tgtcgccact gtgccctggc aagtcatagc
     7081 gatgttcctg gccattggcg tcaaagacac acagttctac ccgctcggca tgagcggaga
     7141 aaagtgtgaa gttgacgccc tgaccgtcgt aatgcgcgcc gaggggagcg ggtttgccaa
     7201 tggcgagttg tgtcattctg cctcccgaac cagccagata gtggccagcg gtggtagtgt
     7261 caggcttagt gaatgctgac gaccgtggct ggcaatctca tcgctgtgta ccgtgccgcc
     7321 attgcctgca ttactgccgt gatagtgcat ggaatcggta ttgaggattt cacgccattt
     7381 gcctggctga ttaatgccga agcgataatc atgacgcggc accggcgtaa agttactggc
     7441 aacgataatt tcgttaccct ctttatcgcg acgcacaaag atcagcaccg agcgttcttt
     7501 gtcatccacc accagccatt caaagccgta cgggtcaaaa tccagttcat gcattgcttt
     7561 atggtggcgg taggtgaggt tcagatcgcg caccagacgc tggacaccgt ggtgccagtt
     7621 atcgccacct tccaacagat gccagtcgag gctggcgtca tggttccact cacggccctg
     7681 ggcaaattcg ttccccatga acagcagttt cttgcccggg aatgcccaca tccagccata
     7741 gtaggcgcgc aggttagcga atttctgcca tgcgtcgccc ggcatgcggt cgagaatcga
     7801 ttttttaccg tggaccactt catcatgcga caacggcagg acgaagtttt cagtgtagtt
     7861 gtagagaatc ccgaaggtga gtttatcgtg atgatactga cgataaaccg ggtcgagctt
     7921 catgtagtcc agggtgtcat gcatccagcc gaggttccac ttgtaccaga agcccagacc
     7981 gcccatatcc tgcggacgag aaacgccagg gaaatcggta gactcctcag ccattgtcac
     8041 cgcaccggaa acctgctcac caagaatacg gttggtatta cgcaagaatt caatcgcttc
     8101 aagattctcg cgcccgccaa attcgttcgg gatccactcc ccctctttac ggctgtagtc
     8161 gcgataaatc attgacgcca ccgcatcgac gcgcagcgca tcaataccaa aacgttcaat
     8221 ccagtaaagc gcgttaccga cgaggaagtt actgacttca cggcgaccat aattgtagat
     8281 cagcgtattc cagtcctgat gatagccttc gcgcggatcg ctgtgttcat acaagttcgt
     8341 gccatcaaat tcggcaagcg caaagtcatc ggtcgggaag tggcctggca cccagtcgag
     8401 aatcacgttc agacccgctg cgtgtgcggc atcaatgaaa taacggaagt cgtcgcgagt
     8461 accaaaacgg cgggttggtg catacaggcc ggttggctga taaccccaac tgccatcgaa
     8521 gggatgctcg ttaatgggca gtagttcgag gtgggtaaag cccatccatt tagcataagg
     8581 caccagttga tcggccagct cgcggtagct caaccagaaa ttgttatcgg tgtggcgacg
     8641 ccaggagccc aggtgaactt cataaataga gattgacgca tcaaactgat tcgctttttt
     8701 gcgctcttca gtctgtacaa ccttttccgg cagcccgcaa ataagagacg cggtttccgg
     8761 gcgcatttgc gcttcgaagg cataagggtc ggacttcaga cgcaagttgc cattggcatc
     8821 aatcatctcg tatttataga gctgaccgtt atgcgcccca gggataaaca gttcccaaat
     8881 gccgctctct ttacgcagac gcatcgggtg acggcgaccg tcccagtagt tgaattgccc
     8941 aaccaccgag acccgacggg cgtttggagc ccaaacagag aaacgcgtac ctgtgacgcc
     9001 atccatagta tctgcatgcg cgcctaaggt ttcatacggg cgcaggtgag taccttcaga
     9061 taatagccag gcatccattt cctggattag cgggccaaaa cggtaaggat catcaatcag
     9121 gttttgctga ccatgccaga caacagccaa ctgatagcgg aaaaaattct tacgtcgcgg
     9181 aatgacaccg ctaaagaatc cccgtgagtc aagacactcc agttttgcga gtttgcgccc
     9241 ggttttcggt tcaatcaccc acacatcggt agcgtcgggt aaaagggcac ggacttccag
     9301 tcccgcggtg gttttatgca ttcccagtac ggaaaaagga tccgcaaaat ggcctgcaat
     9361 tagcgcgtta atcacgtctc tatcgatacg atcggacatg cttgtcttcc tgttttattg
     9421 tgtcacccca tccaattgga tttttgactt ctggttgtga catttttttg acctgaacgg
     9481 cgcagcactc tgtgcatcct ctctgcgtcg tcctcacttc aggtaaggct gtgaatactc
     9541 atgtattcag ccacccttaa agaatagcca atgctctatt taactcccgg taaatcatga
     9601 aacatctgcg cttactcctg tattacgcac taacaggggc ggcatcgcgc cccagattta
     9661 atgaataaag attacgccag ttgacgaagc atccgacgca gcggctccgc ggccccccac
     9721 agcagctggt cacccacggt aaaggctgac aggaactccg gtcccatatt cagcttacgc
     9781 aggcggccta ccggcgtggt cagcgtgccg gtaacggcag ctggggttag ctcacgcata
     9841 gtgatttccc gatcgttcgg aacgactttc gcccacggat tgtgcgcagc cagcagttct
     9901 tcgacggtcg gaatagacac atcttttttc aatttaatag tgaatgcctg gctgtggcag
     9961 cgcaatgccc cgacacgcac acataaacca tctaccggaa ttacggaaga tgtgttgagg
    10021 atcttgttgg tttccgcctg ccctttccac tcttcgcggc tctgaccgtt atcgagctgt
    10081 ttgtcgatcc acggaatcag gctacccgcc agcggcacgc caaagttatc caccggcagc
    10141 tcaccgctac gggttaaggt tgtgactttg cgttcgatat cgagaatagc agaggacggg
    10201 gtcgcgagtt catctgccac atggccatac agatggccca tctgggttaa taactcacgc
    10261 atatgtcgcg caccaccgcc ggaagcggcc tggtaggttg caacggacac ccaatcaaca
    10321 agatcattgg cgaataaacc acccaacgac atcaacatca ggcttacggt acagttaccg
    10381 ccaacaaaag tcctgatgcc attatttaat ccgtcggtaa tgacgtcctg attgacgggg
    10441 tcaagaatga tgatggcgtc atctttcatg cgcagagacg atgctgcgtc aatccagtaa
    10501 ccttgccatc cgctttcacg aagctttgga tagatttcgt tggtataatc gccgccctga
    10561 caggtcacaa tgatatcgag ggcctttagc gcctccagat caaaggcatc ctgaagtgtg
    10621 ccagtggttc cgccaaaaga cggcgcagcc tggccaagct gagaagtaga aaagaagaca
    10681 gggcgaatgg cgtcgaagtc gcgctcttca accatgcgtt gcatgagaac ggagccgacc
    10741 ataccgcgcc agccgataaa accaacattt ttcataagcg ttttttcctg caaagatgtg
    10801 tgctgtataa atgtgccggt ctcctcttgg cacatctttc accatacaaa aagcagccaa
    10861 agtcgcaagt gaaattaatc aatgatagcg aagccatcag taatgcgact tatcctgctt
    10921 tgttagcaca cagaaagtcc gcggcaatta tcagggaatt tgagttatga atgaaatcat
    10981 ttctgcagca gttttattga tcctgattat ggatccgctc ggaaacctgc ctattttcat
    11041 gtccgtactg aaacatactg aaccgaaaag acggcgggca atcatggtgc gagagttgct
    11101 tattgctctc ctggtgatgc tggtgttcct gtttgcgggt gagaaaattc tggcatttct
    11161 cagcctgcga gcagaaaccg tctccatttc tggcggcatc attctgtttc taatcgccat
    11221 taagatgatt ttccccagcg cttcaggaaa tagcagcggg cttccggcag gtgaagagcc
    11281 atttatcgtg ccgttggcaa ttccgttagt cgccgggccg actattctcg ccacgctgat
    11341 gttgttgtct catcagtacc cgaatcagat gggacatctg gtgattgctc tgctgctggc
    11401 ctggggcggc acctttgtca tcctgctaca gtcttcgcta tttttacgtc tgctgggcga
    11461 gaaaggggtg aacgcacttg aacgcctgat gggattgatt ctggtgatga tggcaaccca
    11521 gatgttcctc gacggcattc gaatgtggat gaaggggtaa cgaggagagt ttttcgaaaa
    11581 gaacccggta gcgtcacaac taccgggcga acaaacttaa ctcaacagct ggaacgcaat
    11641 catcccaacg atggcaccaa cagtgccgag gatggtttcc atcatggtcc aggttttcag
    11701 cgtttcggct tcggtcgcgc cggtaaattt accgaacaac cagaaaccgg cgtcgttaac
    11761 gtggctgaca acaatcgaac caccagcgat acaaatcgac agtgccgcca tttgcgcacc
    11821 ggagtagttc agttgttcaa tgaccggcat caccagcccc accgccgtta aacaggcaac
    11881 ggtggcagac ccctgaatga tgcgcactgc agcggccagc acgaagcagg tgatagcaat
    11941 cggcaggccc atgccggtta acgcttcgcc cagtgccgga cctacgccag agtcaaccag
    12001 cacctgtttg aacacgccgc ctgcaccaat caccagcaga atgatccccg ccggttgtag
    12061 cgcgtgaccg caaatctcca tcactttgtc ttttggcatg ccctgacgca ttgccaggcc
    12121 ataaatcgct accagacaag caaccagaat cgcggtaaat ggatgaccaa taaactcgaa
    12181 ccattcgtaa gcagttgatc cttctggcac aaaacgagcg gcaatggttt tcagccccac
    12241 cagcaccagc ggcaacagga tcagcgacag gctgaatccg aaggacggca ttttgccttc
    12301 gccgagatgc ggttcgctga tgtcgtcagg aatatgcaac tccacgtaac ggctgatgaa
    12361 attaccccac agcggcccgg caataatcat tcccggaatt gccgcacaca gaccaatcag
    12421 gatcatccag ccaaagtcgg cattcatctg cgatgccagc aacatcggcg ctggtccagg
    12481 caccagaaat gccgccgctg ccgccacgcc tgcgaataat gggattacca gcttcaccag
    12541 gttcgtaccg gtgtggcgcg ccattgagaa agcaacgcta atcagcagaa ctatcgccac
    12601 ttcaaagaac agcggtagcg cacagaccaa ccccgcaagg ccgatggcat aatgcgcgcg
    12661 gctgtgaccg aaggatttga gcattttgac ggcaatctga tcgactgcgc cggtttcatg
    12721 taagatcttg ccaaacatag ctcccagggc gacaaccacc gccaggaagc cgagggggcc
    12781 tcccatccct ttttccatcg tcgctgcgat tttatcgagc ggcataccgg aaaaaaggcc
    12841 agcccccatg gacaccacca tcaaagccag gaaagcgtgc atacgcgcct tcatgactaa
    12901 aaacagcagc agtaaaacag accctactgc tgttaaaaca agcgttaatg tagtcactac
    12961 ttatttgcct tttttaataa cctcaatggt gcttgccaca acaccttcca gcggttgatc
    13021 gatatccacc accagtacat cggtttcgtc cgcacccggc tcctgcagcg tttcaaactg
    13081 cgtcaccaac atttgggttt taaagaaatg gcctttgcgc gctttcaagc gactttcaat
    13141 cacatcaaaa tcgcctttca ggtagatgaa agagagattc ggattacctt cacgcagcaa
    13201 gtcgcgatag tgctttttca gtgcagaaca gacgatcagc gacactttat tggtgcgcag
    13261 catagcaaac gcggcgtcgt tcagcgcctg caaccagggt ttgcgatcgt cgtcattcag
    13321 cggttcgcca gacgccattt tttcgatatt gcgccgtgga tggaggaaat caccatcaag
    13381 aaacgcggca tgaagttgat gcgccacttc actggcgacc gcagatttgc cgctgcccga
    13441 tacgcccatc aagacgtaaa tgtggtgatc atggttagtc gtgctcaaag tggtgccccc
    13501 acaatacaag aattaaaaat gttacgggta actgttatcg gtaacattgt ccagccggac
    13561 aatatcagaa gcaatatcca tccgtgcctt aagtgtataa gtgtgagcta cttcaaattt
    13621 gtaggcttaa atagatccgc ccggtgacaa ggtgaaacct aaatctaaca ttttcggtgt
    13681 cacagattcg ccacgaatac gcgccagcag gcgttcagcg ccaatactgc ccatccgctc
    13741 acgcggcgtc agcacgctcg ctaaccgcgg ctccatcacc tggccaatgt catgaccgtg
    13801 gaaaccggca atcgccatat cgtcaggaac cttcaatccc agacgctgac attcaaacgc
    13861 cgcgccgacc gccaggtcat cgttggtaca gaacacgcca tccagctgcg gatattcccg
    13921 ccgcgcctgg cgaatcagtt caataccgga agagtaagaa gaagattgct caaccatcac
    13981 gctatacgac accagacctg catccagcat cgcctgttcg tatcccttct gtttgatgat
    14041 agtacgttcg tcgagacgcg cgccgagata ggcaatgtgg caatgcccgc gagcaataat
    14101 ggcggtggtc atctggcgtg ccgcttcaaa gttatcaaaa ccgacggcga tatcgaggca
    14161 cggcgactgg ctgtccatga gttccaccac cgggatcccc gccacttcaa tcatctttaa
    14221 ggtgcgcggc gtgtgggtac gttcggtgag gatcagtccg tcgatattcc atgagagcat
    14281 ggattcgaga cgttcttgct ccatttccgg tttataaccg tagtgagcca gcatggtctg
    14341 ataaccgtgc gcgtcggtga cgctttcgat tccgcgtaat acttccgcga aaacctggtt
    14401 ggtgagagaa ggtaacagga cgccaatcgc ccggctggtg gcgttagaga ggatatcggg
    14461 cgcacgattg ggaatatagc ccagttcatc aagagcggcg gcaatcttgc cgcgtagagc
    14521 gacggaaacc tgctccgggt tgcgtaaaaa acggctgacc gtcattttgg tcacgcctac
    14581 acggtcagcc acatcctgaa gtacgggtct tttctttttc atcgtcctga aggtacaaaa
    14641 gagatagatt ctcttagttt aacacggacg catcagaacc ttcccgggga aaacagtgct
    14701 tgtttacaat tattagatat acactaaatc aattgcatcg cattgtgcta acgacgcggc
    14761 aaaaccccgg aagcgtgcat cagcacgcga ccggagcgag cgaggaagcc tgcaaaaagg
    14821 caggcttcaa agatgccgtt gagttttaaa ccggcggcag atcgaacagt aacacttcgc
    14881 tgtcgctatc cgcatggatg gagattgcct gctcatccca gattgccaga ccatcgctgg
    14941 tcgaggcttt cacgccgtta atggtgacat tgcctttcac cacctggatc cagacgcggc
    15001 gttcagcggc aatctgatgc accgactgct catctttcag caacgcccag cggtacagtt
    15061 ccatatcctg atgcactttc aatgaaccat ctcgcgcatc cggcgagagc accagctgtt
    15121 tgccctgtac ggcatcgaag cgacgctgtt cataacgcgg cgtaataccg ttttcttcag
    15181 gcatgatcca gatctgatac agatgcagac gctcggtgct gcttgggttg tactctgagt
    15241 gacgaatacc cgtacccgca ctcataatct ggaactcacc cgccggaacc tgctctttat
    15301 tgcccatgct gtcctgatgc tcaacagtac cttccagcac gtaggtcaaa atttccatat
    15361 ctttatgcgg gtgagtgccg aagccctgcc ctgcttcaat cacgtcgtcg ttaatcacgc
    15421 gcagcgcgga gaagcccata aagttcggat cgtaatagtt ggcaaaagag aaagtatgcc
    15481 aggagtccag ccagccatga tttgcatgac cacgttcatt tgctttgcgt aagtagatca
    15541 tttgtattcg ccccctgaat gatttcgatg ggcttagtgt ggacccgatc tgcctgggat
    15601 gatagagggt gaaaattgcc ccctctgttc aaaaaaattg aactattcga ggggccatca
    15661 aacttaattc gcgagagtca ctgtggaggg agaggcttgc tcaaatccgc gttcaaggat
    15721 ttccagattg gtaagaactt cagattcctt gacgtaattt ggcgcaccgt gggtgatggt
    15781 ttgatacaac gcatcataaa cgcgcccgta atcgcccatc tccggcttca tctcttctct
    15841 gaccgtcacg ccctcgtcat tgacatactc cggcacaccg accgaatcat ccgctgcgaa
    15901 tcccggttcg cccggcataa tattagcctt caggctggtt tcctgctggt cgataccgta
    15961 tttaataaac gaacctttct taccgtgaac gataaatttc ggataatcga ttttcaccag
    16021 atggctggtt ttgacgatgg ctttcaggtc gccataaaac agttgcgctt caaaggtgtc
    16081 gtcaggattg gctttattac gcaggctgcg gatgtcataa gcgacgtgat ccgggcgacc
    16141 gaacagagaa ataatctggt ccatcgtatg cacaccaagg ccataaaacg cgccatcctg
    16201 cggcagccca ggtttggttt ctgccaccgg gcggtaatag tcaaaatggc tttccacttc
    16261 aacaatctct cccaacttgc cactttcaat cgcttttttc gctgtcagga agcaggagtc
    16321 aaagcgacga ttctgatacg gcgtgacgct cagccctttg cttttcgcca acgcaaacag
    16381 ctccttcgcc tgggcaagcg tcggggtgaa cggtttttcg accagcacat ttttcccggc
    16441 ttccagcgcg cgtttcgcgt actcgaaatg gctgtccgcg tgggtgcaga caacaaccag
    16501 cttaacatcg ggatcgttta gtacttcgtc gagatcgctg atgaaatgga tatgggaata
    16561 aatgggagcc tgttcttccg gcttcgcatg gcgacgaaaa atatgcgcga catgccagct
    16621 atccttgcgg ttaagtacat acggcagatg gtaacgggtg gtgcttttgc cgaagccaat
    16681 aaaggcgcag ttgatgacca tgatttcgtc ctttttaagg tggttattta caccttagcg
    16741 caaagcggac gtggttccta ctggagtgcg cataacgaac acaagcactc ccgtggataa
    16801 attgagaacg aaagatcaaa aaaaagccag cacccggctg gctaagtaat actggaagca
    16861 atgtgagcaa tgtcgtgctt tcaggttctc cgcgagggtc ttcctgatcg cgagacaata
    16921 ataatcattc tcattcgcac ttgtccaaca ctttttgcaa aaaaatgcat ttgactcgca
    16981 tttgaaagtc aatgatgttg aaagggacat ttaccccaaa gaggacaaag gaatgagtga
    17041 gatagtaata cgccacgcag aaacacggga ttacgaggcc atcaggcaga ttcacgccca
    17101 gccggaggtg tattgcaaca cactacaggt gcctcatcct tccgatcata tgtggcagga
    17161 gcgactcgcc gatcgtcccg gcatcaagca actcgtcgcc tgtattgatg gagacgtcgt
    17221 gggccatctc accattgacg tgcaacagcg cccacgccgc agtcatgttg ccgattttgg
    17281 tatctgtgtc gactctcgtt ggaagaaccg cggcgtcgcc agcgccctga tgcgagagat
    17341 gattgaaatg tgcgacaact ggttgcgggt agatcgcatt gaactaaccg tgtttgtcga
    17401 taacgccccg gcaattaagg tctataaaaa atacggcttt gaaattgaag ggactggtaa
    17461 gaagtacgca ttgcgtaatg gtgaatatgt cgatgcatat tatatggcgc gggtgaagta
    17521 agatagtgcc ctttttctga gatggaaaaa gggtgtcatt caaaatcgac ataccttcct
    17581 ttaaggtatt tatttaccca atacatatat tgagatttaa ctcataatta cttcctaaag
    17641 tgtaatattt tattttttaa tatatacgcc tacaatttcc tggagtaaat aaataacaat
    17701 taacaagcat aatattgcca ttgataaaat agcatgccat aaaaggactt ttcagggatg
    17761 agtaatattg tttacctgac agtaacggga gaacaacaag gaagcatctc cgcaggttgt
    17821 gggacttctg agtctacagg taatcgttgg cagagcgggc atgaggatga aatatttaca
    17881 ttctcactct taaataatat taataatacg gggcttggtt cacagttcca tggtataaca
    17941 ttttgtaaat taattgataa aagcactcca ttatttatta attccattaa caataatgaa
    18001 caattattta tgggatttga cttctatcga ataaatagat ttggtagatt ggaaaagtat
    18061 tattatatac aactaagagg cgctttttta tcggctattc atcaccagat cattgaaaac
    18121 caactggata cagaaacaat aactattagt tatgaattta tcctctgtca acatcttatc
    18181 gcaaataccg agttcagcta tttggcactc cctgaaaatt ataaccgatt gtttttacca
    18241 aattcaaaaa accaaacaaa taatcgtttc aaaacgttaa acagcaaagc tattggcagg
    18301 ctacttgctg ctggtggcgt atacaatggg aacattgaag gattcagaga tactgcggaa
    18361 aaactgggtg gagatgcaat aaaaggctat gatcaaatac taaatgaaaa aacagcgggc
    18421 atagcgatag caacagcatc tattctttta acaaagcgtt ctaatgttga tacatataca
    18481 gaaataaata gttacttagg caaacttaga ggtcaacaaa aacttcttga tggtatagac
    18541 ataatagaaa taatatacat taagagacct tcaaaagact tagctaactt acgaaaggag
    18601 tttaataaaa ctgtaagaaa aaattttctt atcaaacttg caaaaacctc cgaagcatct
    18661 ggaagattca acgccgaaga ccttttaaga atgagaaagg gcaatgttcc tctaaattat
    18721 aatgttcacc ata
//
LOCUS       DAJVUJ010000078        18498 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.078, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000078 DAJVUJ010000000
VERSION     DAJVUJ010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18498
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.078"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..893
                     /locus_tag="OQQ48_002384"
     CDS             <1..893
                     /locus_tag="OQQ48_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1953130.1"
                     /translation="WFNIKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFIT
                     IGVLGIFLAIGWYMLYRNREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTM
                     WGMMLGFSGINYTAWLYLAWLPGYLQTAYNLDLKSTGLMTAIPFLFGAAGMLVNGYVT
                     DWLVKGGMAPIKSRKICIIAGMFCSAAFTLIVPQATTSMTAVLLIGMALFCIHFAGTS
                     CRGLIHVAVASRMTASVGSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTA
                     AGALAYIFLVRQPINDPRKD"
     gene            complement(942..2597)
                     /gene="tsr"
                     /locus_tag="OQQ48_002385"
     CDS             complement(942..2597)
                     /gene="tsr"
                     /locus_tag="OQQ48_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HCS1953131.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQRETSAVVKTVTPAAPRKMAVADSEENWETF"
     gene            complement(2716..3162)
                     /gene="hpaR"
                     /locus_tag="OQQ48_002386"
     CDS             complement(2716..3162)
                     /gene="hpaR"
                     /locus_tag="OQQ48_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HCS1953132.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEAIPGDNE"
     gene            3434..4723
                     /locus_tag="OQQ48_002387"
     CDS             3434..4723
                     /locus_tag="OQQ48_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HCS1953133.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPGDRVRVLAEGFPPLENPVVDEREVTTRKSFPTQ
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEDTAK"
     gene            4720..6179
                     /gene="hpaE"
                     /locus_tag="OQQ48_002388"
                     /pseudo
     CDS             4720..6179
                     /gene="hpaE"
                     /locus_tag="OQQ48_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
     gene            6181..7032
                     /gene="hpaD"
                     /locus_tag="OQQ48_002389"
     CDS             6181..7032
                     /gene="hpaD"
                     /locus_tag="OQQ48_002389"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HCS1953134.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     ADEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            7042..7422
                     /locus_tag="OQQ48_002390"
     CDS             7042..7422
                     /locus_tag="OQQ48_002390"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HCS1953135.1"
                     /translation="MPHFIVECSDNIREEADLPGLFAKVNPTLAATGIFPLAGIRSRV
                     HWVDTWQMADGQRDYAFVHMTLKIGAGRSLESRQQAGEMLFELIKTHFAALMESRLLA
                     LSFEIEELHPTLNFKQNNVHALFK"
     gene            7533..8336
                     /gene="hpaH"
                     /locus_tag="OQQ48_002391"
     CDS             7533..8336
                     /gene="hpaH"
                     /locus_tag="OQQ48_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HCS1953136.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            8347..9135
                     /gene="hpaI"
                     /locus_tag="OQQ48_002392"
     CDS             8347..9135
                     /gene="hpaI"
                     /locus_tag="OQQ48_002392"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HCS1953137.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLSDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     EAVHATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIRESGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATAVKPGVY"
     gene            9310..10686
                     /gene="hpaX"
                     /locus_tag="OQQ48_002393"
     CDS             9310..10686
                     /gene="hpaX"
                     /locus_tag="OQQ48_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HCS1953138.1"
                     /translation="MSDTSPAIPESIDPANQHKALTAGQQAVIKKLFRRLIVFLFVLF
                     IFSFLDRINIGFAGLTMGRDLGLSATMFGLATTLFYAAYVIFGIPSNIMLSIVGARRW
                     IATIMVLWGIASTATMFATGPTSLYVLRILVGITEAGFLPGILLYLTFWFPAYFRARA
                     NALFMVAMPVTTALGSIVSGYILSLDGVMALKGWQWLFLLEGFPSVLLGVMVWFWLDD
                     SPDKAKWLTKEDKKCLQEMMDNDRLTLVQPEGAISHHAMQQRSMWREIFTPVVMMYTL
                     AYFCLTNTLSAISIWTPQILQSFNQGSSNITIGLLAAVPQICTILGMVYWSRHSDRRQ
                     ERRHHTALPYLFAAAGWLLASATDHNMIQMLGIIMASTGSFSAMAIFWTTPDQSISLR
                     ARAIGIAVINATGNIGSALSPFMIGWLKDLTGSFNSGLWFVAALLVIGAGIIWAIPMQ
                     SSRPRATP"
     gene            10696..11582
                     /gene="hpaA"
                     /locus_tag="OQQ48_002394"
                     /pseudo
     CDS             10696..11582
                     /gene="hpaA"
                     /locus_tag="OQQ48_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224927.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
     gene            11833..13395
                     /gene="hpaB"
                     /locus_tag="OQQ48_002395"
     CDS             11833..13395
                     /gene="hpaB"
                     /locus_tag="OQQ48_002395"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HCS1953139.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSANDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDADGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHAALQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQHIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            13413..13925
                     /locus_tag="OQQ48_002396"
     CDS             13413..13925
                     /locus_tag="OQQ48_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HCS1953140.1"
                     /translation="MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            14318..16468
                     /gene="btsT"
                     /locus_tag="OQQ48_002397"
     CDS             14318..16468
                     /gene="btsT"
                     /locus_tag="OQQ48_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HCS1953141.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            16599..16802
                     /locus_tag="OQQ48_002398"
     CDS             16599..16802
                     /locus_tag="OQQ48_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCS1953142.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            16813..17769
                     /gene="yjiA"
                     /locus_tag="OQQ48_002399"
     CDS             16813..17769
                     /gene="yjiA"
                     /locus_tag="OQQ48_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HCS1953143.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEDEIRAAFAGLKK"
     gene            complement(17871..>18498)
                     /locus_tag="OQQ48_002400"
     CDS             complement(17871..>18498)
                     /locus_tag="OQQ48_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HCS1953144.1"
                     /translation="PGSAILNVWLTSMLKRTVVAAVPMPFDKWYSASGYETTQDGPTG
                     SLNISVGAKILYEAVQGDKSPIPQAVDLFAGKPQQEVVLAALEDTWETLSKRYGNNVS
                     NWKTPAMALTFRANNFFDVPQAAAEETRHQAEYQNRGTENDMIVFSPTTSDRPVLAWD
                     VVAPGQSGFIAPDGTVDQHYEDQLKMYENFGRKSLWLTKQDVEAHKES"
BASE COUNT         4227 a         5121 c         4934 g         4216 t
ORIGIN      
        1 actggttcaa catcaaagag cgcggacgcc cgatgggctt cttcaacgca gcttctacca
       61 ttggcgttgc cgtaagccca ccgattctgg cggcgatgat gctggtgatg ggctggcgcg
      121 ggatgtttat taccattggt gtactgggga tttttctcgc catcggctgg tatatgctct
      181 atcgcaaccg cgagcacgta gaactgactg ccgttgagca agcttatctc aatgcaggta
      241 gcgtcaatgc ccgccgagat ccgctcagtt ttgccgaatg gcgcagcctg ttccgtaacc
      301 gtacaatgtg gggaatgatg ctcggattca gtggcatcaa ctacactgcg tggctgtatc
      361 tggcctggct tcctggttac ctgcaaacag cctataacct ggatttaaaa agcacagggt
      421 tgatgacggc tatccctttc ctgtttgggg ctgccgggat gctggtcaac ggttacgtta
      481 ccgactggct ggtcaaaggg ggaatggctc cgattaaaag ccgtaagatc tgcattattg
      541 ccgggatgtt ctgttctgcc gcctttacgc tgatagtacc acaagcgaca acatccatga
      601 cggcggttct gctgattggc atggcactgt tctgtattca ctttgccgga acatcctgcc
      661 ggggcttgat ccacgtcgca gttgcttctc gcatgactgc gtcggtgggc agtatccaga
      721 actttgccag cttcatctgc gcctcttttg cgccgatcat tactggtttt attgttgata
      781 ccacccactc attccgtctg gcactaatca tctgcggttg cgtcaccgca gcgggggcac
      841 tggcgtacat cttcctggtt cgtcagccga tcaacgaccc acgtaaagat taatctcctt
      901 atgcccgata acattttgct tatcgggcat tttcatggcg attaaaatgt ttcccagttc
      961 tcctcgctat ctgccacggc cattttacgc ggcgcagctg gcgtcacggt ttttaccaca
     1021 gccgatgttt cacgctgctg ttgctgaatc cggaacactg ccactgcttc ggtcaggcga
     1081 ctggcctgct cttccagcgc ggcggcggca gcggcagact cttccaccag cgcggcgttc
     1141 tgttgagtta cccggtccat ctcagcaacc gctaagccaa cctgatcgat accacggctc
     1201 tgctcatcag aagcagaagc aatttcgccc ataatgtccg tcacgcgggt cacggcgctg
     1261 acaatctccg ccattgtttc cccggcgctt tcgaccagcg tagagccaac atccactttc
     1321 cccaccgagt cttcaatcag gcttttaatt tcacgagccg cctgggcgct gcgctgggcc
     1381 agattacgca cttctcccgc gaccaccgca aaaccgcgcc cttgctcacc cgcacgcgca
     1441 gcctcaaccg ccgcgttcaa agccagaata ttggtctgga aggcaatgcc gtcaattacg
     1501 ctgataatat cggcgatttt ctgcgaactg gtggagatat cgcgcatagt ctgtaccacg
     1561 ttatctacca ctttgccgcc gcgttgcgcc gtttcagaag cacttaacgc cagatggctg
     1621 gcctggcgcg cattttcggc gttctgcttc accgttgcgg tcagttgctc catgctggct
     1681 gccgtctctt ccagcgaagc ggcctgttgc tcggtgcgcg aagagagatc gttattgccg
     1741 gtggcgattt cgctggcacc gctatagatg gcattggccc cgttgcgcac atcaccgacg
     1801 gtacgcatca gctctccctg catatggcgc aaactctctg ccagttgccc catctcatta
     1861 gagccatcca cctcaatcgg tttcaccaga tcgccgcctg caatatgacg aatgctgtca
     1921 atcaggcgat tcattggcgc taccagcgag gctttaatac cgaaccagac ggcgaagatg
     1981 accgccagta cgacgatcat cacgcccacc agaatccaca tcgcctggct gtaggaggca
     2041 ttgttatcgc tgacggcgat atcatagagc cgatcgtttt gctccatgta agccacatac
     2101 tgcttctcga aaccgtcctg atatccctgg gtcggctgat caaagaactc gttgattttg
     2161 cctgcaccta acagttggat cagctccgcc agcgcattgt gataaatatc gtaattacgt
     2221 ttgatctctg ccgctgcggc ggtgctctga cgcgggtcac gcggcaacgc ttcgtaatcc
     2281 gcccagtttt tttccgcctg tttcagcgaa atactggcac tctgcatcag ctcagcaacg
     2341 gttgaaccgc taccaatatt attctgatcc atcatgtagc ggatacccgc gcggttgagg
     2401 gtgttacgcg tctgcaacaa cgcgacccag ctgccattca gcgtggattg ctgctggcga
     2461 atggtttgta aaacagtgaa attttctttg tcattcttta aggcattaaa gaacagaccg
     2521 cctgatgtca gttgtaaaag gccaaaaacg gccaaaacca gcagtaagct ggtcacgatc
     2581 ttgatacgtt ttaacatggt ttctctttcc tgtggaccga tgcaagattt tcggcttgga
     2641 aagcaaaaac tttatgaaaa ggtgtaaccc gccatccatg gaaaaaccag gcgctggtta
     2701 tcagatacta aaaagttatt cattatcgcc cgggatggct tcttgtcggg aattgcccag
     2761 agcaataaat tcctctaaca gatgggttaa ctgttgcatt ttctcggcag taaactgagc
     2821 ttcaatctgc cgataggctt cttcaatctg tgtctgagcg cggttataca gcgcctgccc
     2881 ctctttggtt agcgagatat agagcttccg ctgatcattg atcggcttta atcgcaacac
     2941 taaaccgtcg cgttccatcc gcgtgagaat cccggtcagg cttgggcgca aaatgcaggc
     3001 gcgatacgcc agatcgtgaa aatccattga tgggctttcc gccagaatac gcacgatgcg
     3061 ccactgttgc tcagtcaaat tatgccgttt aacgattggg cgaaaataac tcatcgccgc
     3121 ttctcgcgcc tggagcaacg caatggttag tgagtcgtgc attatctttc ccctgaaaaa
     3181 ttcaaaatca ttaatataga aacagttaat cgtcacgtta ccgggactgc gctgcccctg
     3241 caattcctga ttttatcaaa attgatgata ttttattaac ttactgatat ttaatatttt
     3301 attatcttta aatgtaaata gtttgttaat tagatcacat ttacatcact tacttttgcc
     3361 aaactacgca atcgggaata aaagcaatca ttaacatatt aatgattaag ataatcgaat
     3421 ccgaggagtg gtaatgaaag gcactatctt cgccgtagcg ttgaaccatc gcagccaact
     3481 tgatgcatgg caggaagcgt tccagcaatc cccctacaaa gccccgccta aaactgcggt
     3541 ctggtttatt aaaccgcgca atacggtgat tggttgcggt gaaccgattc cctttccaca
     3601 gggtgaaaag gtactgagcg gtgcgactgt tgcgctgatt gtgggaaaaa cggcgacgaa
     3661 agtacgtgaa gaagatgcgg cagagtacat cgccggatat gcgctggcta acgacgtcag
     3721 cctgccggaa gagagctttt accgcccggc aatcaaagca aaatgtcgtg atggattctg
     3781 ccccattggc gaaaccgtgg ctctcagcaa tgtcgataat ctgaccatct ataccgagat
     3841 caacgggcgt cctgccgatc actggaatac cgccgattta caacgtaacg ccgcgcagtt
     3901 gctgagcgcc ctgagcgaat ttgccacgct gaatccaggc gacgccattc tgctcggcac
     3961 gccacaggcg cgcgtggaaa tacagccagg cgatcgcgtt cgtgttctcg cagaaggttt
     4021 cccgccgctg gaaaatccgg tagtggacga acgtgaagtg accacgcgca agagcttccc
     4081 aacgcagcca cacccgcacg gcacgctgtt tgccctcggc ctgaactacg ccgaccacgc
     4141 cagcgaactg gaatttaagc caccggaaga accgctggta ttcctgaaag cgccaaatac
     4201 cctcactggc gataaccaga cctccgtgcg tccaaacaat attgaataca tgcactatga
     4261 agcggagctg gtggtagtta ttggcaaaca ggcgcgtaac gtcagcgaag ccgatgccat
     4321 ggattatgtc gcgggctaca ccgtgtgtaa cgactacgcc attcgtgact atctggaaaa
     4381 ctactaccgc cctaacctgc gggtaaaaag ccgcgacgga ctgacgccga tgctttcaac
     4441 catcgtgccg aaagaggcga tcccggaccc gcataatctg acccttcgca ccttcgtcaa
     4501 cggcgagtta cgccagcaag gcaccaccgc cgatctgatc ttcagcgtgc ccttcctgat
     4561 cgcctactta agtgaattta tgaccctgaa tccgggcgac atgatcgcca ccggcacacc
     4621 caaaggctta tctgacgtgg tgcctggcga tgaagtagtg gtggaagtag aaggcgtggg
     4681 ccgcctggtg aaccgaattg tgagtgagga tacagcgaaa tgaaaaaagt aaatcattgg
     4741 atcaacggca aaaatgttgc aggtaacgac tacttccaga ccaccaatcc ggcaacgggt
     4801 gaagtgctgg cggatgtggc ctctggcggt gaagcggaga tcaatcaggc ggtagcggca
     4861 gcgaaagagg cgttcccgaa atgggccaat ctgccgatga aagagcgtgc gcgcctgatg
     4921 cgccgtctgg gcgatctgat cgaccagaac gtgccagaga tcgccgcgat ggaaaccgcg
     4981 gacaccggcc tgccgatcca tcagaccaaa aatgtgttga tcccacgcgc ttcccacaac
     5041 tttgaatttt tcgcggaagt ctgccagcag atgaacggca agacctatcc ggttgacgac
     5101 aagatgctca actacacgct ggtgcagccg gtgggcgttt gtgcgctggt atcgccgtgg
     5161 aacgtaccgt ttatgaccgc cacatggaag gtcgcgccgt gtctggcgct gggcaatacc
     5221 gcggtactga aaatgtcgga actctccccg ctgaccgcct gggtgagctg gcgctggaag
     5281 ccggtattcc ggcaggcgtg ctgaacgtgg tacagggcta cggcgcaacc gcaggggatg
     5341 cgctggttcg tgatcatgac gtacgtgccg tgtcgttcac cggcggtacg gccaccgggc
     5401 gcaacatcat gaaaaacgcc gggctgaaaa aatactccat ggaactgggc ggtaaatcgc
     5461 cggtgctgat ttttgaagat gccgatattg aacgcgcgct ggacgccgcc ctgttcacca
     5521 tcttctcgat caacggcgaa cgctgcaccg ccggttcacg catctttatt cagcaaagca
     5581 tctacccgga attcgttaaa cgctttgccg aacgcgccaa ccgtctgcgc gtcggcgatc
     5641 cgaacgatcc gaatacccag gttggcgcgc ttatcagcca gcagcactgg gaaaaagtct
     5701 ccggctatat ccgtctcggc attgaagaag gcgccaccct gctggcgggc ggcccggata
     5761 aaccgtctga cctgcctgca cacctgaaag gcggcaactt cctgcgccca acggtgctgg
     5821 cagacgttga taaccgtatg cgagtcgccc aagaagagat tttcgggccg gtcgcctgcc
     5881 tgctgccgtt taaagacgaa gctgaaggct tacgcctggc aaacgacgtg gagtacggcc
     5941 tcgcgtcgta catctggaca caggatgtca gcaaagtgtt acgcctggcg cgtggcattg
     6001 aagctggcat ggtgttcgtc aacacccaga acgtgcgtga cctgcgccag ccatttggcg
     6061 gcgtaaaagc ctccggcacc gggcgtgaag gcggtgagta cagcttcgaa gtgttcgcgg
     6121 aaatgaagaa cgtctgcatt tccatgggcg accatccaat tccgaaatgg ggagtctgat
     6181 atgggtaagt tagcgttagc cgccaagatc acgcacgtac cgtcgatgta tctctctgaa
     6241 ctgccaggga aaaaccacgg ttgccgccag ggcgcgatcg acgggcataa agagatcagc
     6301 aagcgttgcc gggaaatggg cgtcgatacc attatcgttt tcgataccca ctggctggtc
     6361 aacagtgctt atcacatcaa ctgtgcagac cattttgaag gcgtctacac cagcaacgag
     6421 ctgccgcatt ttatccgcga tatgacctac aactacgagg gcaacccgga gttggggcag
     6481 cttattgccg atgaagcctt aaagctcggc gtgcgggcaa aagcgcacaa cattcccagc
     6541 ctgaaactgg aatacggcac gctggtgccg atgcgctaca tgaatgaaga caagcacttc
     6601 aaagtggtct ccatttcggc tttctgcacg gttcacgatt ttgccgacag ccgcaagctg
     6661 ggcgaagcga ttctgaaagc gatcgaacag tacgacggca ccgtggcggt ccttgccagc
     6721 ggttcgttat cgcaccgctt tattgacgat cagcgtgcag aagaagggat gaacagctac
     6781 acccgcgagt tcgaccgcca gatggacgag cgcgtggtga agttgtggcg cgaaggccag
     6841 ttcaaagagt tctgcaatat gctgccggag tacgccgact actgctacgg cgaaggcaat
     6901 atgcacgaca cggtgatgct gctggggatg ctcggctggg ataaatacga cggcaaggtg
     6961 gagtttatca ccgagctatt cccaagctct ggcaccggtc aggttaacgc tgttttcccg
     7021 cttcccgcgt aaggacgttt tatgccgcac tttatcgttg aatgcagtga caacatccgc
     7081 gaagaagccg acctgccggg gttgttcgcc aaagtgaatc cgacgctggc agccacgggt
     7141 atttttccgc tggcgggtat tcgcagccgc gtgcattggg tcgatacctg gcagatggcc
     7201 gacgggcagc gtgattatgc cttcgtgcat atgacgttga aaatcggcgc aggtcgcagc
     7261 ctggaaagcc gtcagcaggc gggtgaaatg ctgtttgaac tgattaaaac gcacttcgcc
     7321 gccctgatgg agagccgcct gctggcgttg tcgtttgaga ttgaagagct gcatccgacg
     7381 ctgaatttta aacaaaacaa cgtgcacgca ttgtttaagt agcgcgcaga ttgcccggtg
     7441 gcgctgcgct taccgggcct acaaaacccc aaaccgtaca ccgtaggccg gataaggcgc
     7501 agccgcatcc ggcaatgcca caggatatcg ctatgttcga taaacacacc cacaccctga
     7561 tcgcccagcg tctggatcag gcagaaaaac agcgcgaaca gatccgcgcg atctcgctgg
     7621 attacccaga gatcaccatc gaagacgctt acgcggtgca gcgcgaatgg gttcgcctga
     7681 aaatcgccga aggccgtacg ctgaaaggcc acaaaatcgg tctgacctca aaagcaatgc
     7741 aggccagctc gcagatcagc gaaccagatt acggtgccct gctggatgac atgttcttcc
     7801 acgatggcag cgatatcccg accgatcgct ttatcgtgcc gcgcattgaa gtggagctgg
     7861 ctttcgtgct ggcaaaaccg ctgcgtgggc caaactgcac gctgttcgac gtctacaacg
     7921 ccacggacta cgtgatcccg gcgctggagc tgatcgacgc tcgctgccac aacatcgatc
     7981 cggaaaccca gcgcccgcgc aaagtgttcg acaccatttc tgataacgcc gccaacgccg
     8041 gggtgatcct cggtggtcgt ccgatcaaac cagacgaact ggatctgcgt tggatctccg
     8101 ccctgatgta tcgcaatggc gtgattgaag aaaccggcgt cgccgctggc gtgctgaatc
     8161 atccggcaaa cggcgtggcc tggctggcga acaaactcgc accgtatgac gtacaactgg
     8221 aagccgggca aatcattctc ggtggttcgt tcacccgccc ggttccggcg cgtaagggcg
     8281 acaccttcca cgtcgattac ggcaacatgg gttccattag ctgccgcttt gtttaaggag
     8341 agaacgatgg aaaacagctt taaagcggcg ctgaaagcag gccgtccgca gatcggatta
     8401 tggctggggc tgagtagcag ctacagcgca gagttactgg ccggagcagg attcgactgg
     8461 ttgttgagcg acggtgagca cgcgccgaat aacgtgcaaa ccgtgctcac ccagctacag
     8521 gcgattgcgc cctaccccag ccagccggta gtacgtccgt cgtggaacga tccggtgcaa
     8581 atcaaacaac tgctggacgt cggcacacaa accttgctgg tgccgatggt acaaaacgct
     8641 gacgaagccc gtgaagcggt acacgccacc cgttatcccc ccgccggtat tcgcggtgtg
     8701 ggcagtgcgc tggcccgcgc ctcgcgctgg aatcgtattc ctgattacct gcaaaaagcc
     8761 aacgatcaaa tgtgcgtgct ggtgcagatc gaaacgcgtg aggcaatgaa gaacttaccg
     8821 cagattctgg acgtggaagg cgtcgacggc gtgtttatcg gcccggcgga tctgagcgcc
     8881 gatatgggtt atgccggtaa tccgcagcac ccggaagtac aggccgccat tgagcaggcg
     8941 atcgtgcaga tccgtgaatc gggcaaagcg ccggggatcc tgatcgccaa tgagcaactg
     9001 gcaaaacgct atctggaact gggcgcgctg tttgtcgccg tcggcgttga caccaccctg
     9061 ctcgcccgcg ccgctgaagc gctggcagca cggtttggtg cgcaggccac cgccgtgaag
     9121 cccggcgtgt attaatgccg gatgcgctgc acttatccgg cctacactcg caccaaaccg
     9181 taggccggat aaggcgttta cgccgcatcc ggcaaaaagc cgtaccaaat cgcggaccgg
     9241 ataaggcgtt acgccgcatc cgccataaac cttgtcgtac cctacaaaaa tcccattaga
     9301 ggaagaaaaa tgagcgacac ctcacctgcc ataccggaga gtatcgatcc ggcgaatcag
     9361 cataaagcgc tgactgccgg acaacaggcg gttattaaga agctatttcg ccgcctgatc
     9421 gtctttctgt tcgtgctgtt tatcttctcg ttccttgatc gcatcaacat cggctttgcc
     9481 ggactcacga tgggacgcga cctcggtctg agcgccacca tgtttggcct cgctaccacc
     9541 ctgttctatg ccgcttatgt catcttcggc attcccagca acattatgct gagtattgtc
     9601 ggtgcgcggc gctggatcgc caccatcatg gtgctctggg gcatcgcctc tactgccacc
     9661 atgtttgcca ctggccccac cagcttgtac gtactgcgta tactggttgg cattaccgaa
     9721 gccggctttc tgcctggtat tctgctgtat ttaaccttct ggtttccggc ctatttccgc
     9781 gcccgtgcca acgccttgtt tatggtagca atgccggtaa cgacagcgtt gggctcgatc
     9841 gtttccggct acattttgtc gctggatggc gtcatggcat taaaaggctg gcagtggctg
     9901 tttttgctgg aaggcttccc gtcggtatta ctcggcgtca tggtgtggtt ctggcttgat
     9961 gactcaccgg acaaagctaa gtggctgacg aaagaagaca aaaaatgcct gcaagagatg
    10021 atggataacg atcgtctgac gctggttcag ccagagggag ccatcagcca ccacgccatg
    10081 caacaacgca gtatgtggcg ggagatcttc actccggtgg tgatgatgta taccctggcg
    10141 tatttctgcc tgaccaacac acttagtgcg atcagcatct ggacaccgca gatcctgcaa
    10201 agttttaatc agggcagcag taatatcacc atcggcctgc tggccgccgt accgcagatt
    10261 tgtaccattc tcgggatggt ctactggagc cgtcactcag atcgccgcca ggaacgaagg
    10321 catcacaccg cccttcctta tttgttcgct gccgcaggtt ggttactggc ttcggcaact
    10381 gatcacaaca tgatccagat gctggggatc attatggctt cgaccggatc attcagcgca
    10441 atggcgattt tctggacaac accggatcag tccatcagcc tgcgggcacg agcgatcggt
    10501 attgcggtga tcaacgccac tggcaacatt ggttcagcgt taagtccgtt tatgatcggc
    10561 tggttgaaag atttgaccgg cagctttaac agtggattgt ggtttgttgc cgcgctgctg
    10621 gtgattggtg cggggattat ctgggcgatt ccaatgcagt cctcccgtcc gcgagcgacc
    10681 ccgtaaggaa cgacgatgtg tgaccgtcag attgccaata ttgatatcag caaagagtac
    10741 gatgaaagcc tgggcacgga cgatgtgcat tatcagtcgt tcgcccgcat ggcggcattt
    10801 tttggccgcc atatgctgcc acatcgccac gaacagtact ttcagatgca tttcctcaat
    10861 agcggacaga ttgagctaca gcttgacgat catcgctact cggtggaagc gcccctgttt
    10921 gtcctgacgc cgccgtcagt acctcatgcg tttattacgg agtctgacgc cgacggtcat
    10981 gtgttgacgg tacgggaaga tctgatctgg cccctgctgg aagttcttta tccgggcact
    11041 cgggaaacct tcggcctgcc gggggcctgt cactggcaga taaacccgac gaactggcgg
    11101 cgctggaaca ctattggcaa ctgatagagc gggaatcggt ggaacaactg cctggacggg
    11161 aacacaccct gacgttactg gcacaggcag tgttcaccct actgctgcgt aacgcaaaac
    11221 tcgacgacca tgccgccagc gggatgcgcg gagaattaaa actgttccag cgttttaata
    11281 tgctgattga aagccatttt catcagcact ggacagtacc ggattacgct aacgaactgc
    11341 atatcaccga atcacgactc acggacatct gccgtcgctt tgccaaccgt ccgccaaaac
    11401 ggttgatttt cgacaggcag ctgcgagaag ccaagcggct gctgctgttt tctgataacg
    11461 ccgtgaacaa tattgcctgg caactcggtt ttaaggatcc ggcttatttt gcgcgctttt
    11521 ttaatcgctt agtcggttgc tcgcccagtg cttatcgcgc caaaaaagta cctgtgacgt
    11581 gaaaatccct tagccttaac gggaaaccag gcaccacctg ctattcccct ttctccgcct
    11641 ggagcaaggg gatttagtaa cgtcaaaaat cgctaaaagc gacctcgatc acaaaaccgt
    11701 acccgacctg aaaagtacca gccattgtcc caaaagtctc ttcccgtaat cgcccctgca
    11761 agctttaatt gaataacaaa aacaacacac aaaattaaca atcgtattcc gattaatact
    11821 gtagaggtcg acatgaaacc agaagatttc cgcgccagta cccaacgtcc gttcaccggg
    11881 gaagagtatc tgaaaagcct gcaggatggt cgcgagatct atatctatgg cgagcgagtg
    11941 aaagacgtca ctactcatcc ggcatttcgt aatgcggcag cgtctgttgc ccaactgtac
    12001 gacgcgctac acaaaccgga gatgcaggac tctctgtgct ggaacaccga caccggcagc
    12061 ggcggctata cccataaatt cttccgcgtg gcgaaaagtg ccaacgacct gcgccagcaa
    12121 cgcgatgcca tcgctgagtg gtcacgcctg agctatggct ggatgggccg taccccagac
    12181 tacaaagctg ctttcggttg cgcactgggc gcgaatccgg gcttttacgg tcagttcgag
    12241 cagaacgccc gtaactggta cacccgtatt caggaaactg gcctctactt taaccacgcg
    12301 attgttaacc caccgatcga tcgtcatttg ccgaccgata aagtaaaaga cgtttacatc
    12361 aagctggaaa aagagactga cgccgggatt atcgtcagcg gtgcgaaagt ggttgccacc
    12421 aactcggcgc tgactcacta caacatgatt ggcttcggct cggcacaagt gatgggcgaa
    12481 aacccggact tcgcactgat gttcgttgcg ccaatggatg ccgatggcgt aaaattaatc
    12541 tctcgcgcct cttatgagat ggtcgcgggt gctaccggct cgccatacga ctacccgctc
    12601 tccagccgct tcgatgagaa cgatgcgatt ctggtgatgg ataacgtgct gatcccatgg
    12661 gaaaacgtac tgatctaccg cgatttcgat cgctgccgtc gctggacaat ggaaggcggt
    12721 tttgcccgta tgtatccgct gcaagcctgt gtgcgcctgg cagtgaaatt agacttcatt
    12781 acagcactgc tgaaaaaatc actcgaatgt accggcaccc tggagttccg tggtgtacag
    12841 gccgatctcg gtgaagtggt ggcgtggcgc aacaccttct gggcattgag tgactcgatg
    12901 tgttctgaag cgacgccgtg ggtcaacggg gcttatttac cggatcatgc cgcactgcaa
    12961 acctatcgcg tactggcacc aatggcctac gcgaagatca aaaacattat cgaacgcaac
    13021 gttaccagtg gcctgatcta tctcccttcc agtgcccgtg acctgaacaa tccgcagatc
    13081 gaccagtatc tggcgaagta tgtgcgcggt tcgaatggta tggatcacgt ccagcacatc
    13141 aagatcctca aactgatgtg ggacgccatt ggcagcgagt ttggtggtcg ccacgaactg
    13201 tatgaaatca actactccgg tagccaggat gagattcgcc tgcagtgtct gcgccaggca
    13261 caaagctccg gcaatatgga caagatgatg gcgatggttg atcgctgcct gtcggaatac
    13321 gaccagaacg gctggactgt gccgcacttg cacaacaacg acgatatcaa catgctggat
    13381 aagctgctga aataacgcag caggaggtta agatgcaatt agatgaacag cgcctgcgct
    13441 ttcgtgacgc gatggccagc ctgtcggcag cggtaaatat tatcaccacc gagggcgacg
    13501 ccggacaatg cgggattacg gcaacggccg tctgctcggt cacggataca ccaccatcgc
    13561 tgatggtgtg cattaacgcc aacagtgcga tgaacccggt ttttcagggc aacggtaagt
    13621 tgtgcgtcaa cgtcctcaac catgagcagg aactgatggc acgccacttc gcgggcatga
    13681 caggcatggc gatggaagag cgttttagcc tctcatgctg gcaaaaaggt ccgctggcgc
    13741 agccggtgct gaaaggttcg ctggccagtc ttgaaggtga gatccgcgat gtgcaggcaa
    13801 ttggcacaca tctggtgtat ctggtggaga ttaaaaacat catcctcagt gcagaaggtc
    13861 acggacttat ctactttaaa cgccgtttcc atccagtgat gctggaaatg gaagctgcga
    13921 tttaagctcc agccccttca cgtattcgtc gaaggggcat ttttattgtg atctcaatca
    13981 catatccacc cgatgaataa ttcttgccca accgctcacc gccattcctg accgcttacg
    14041 tccctaaaat aaccactcag ttatttacct tactttacgc gcgcgtaact ctggcaacat
    14101 cactgcagga tagcggtcaa tttacctcct caaacgcaac gcaaacctag aacggcttcg
    14161 gcctatgatt aacaaattac tctatgaatt ttggtttgca ggaaggcagc aagtgagtga
    14221 atccccggga gcttacaaca gtaagtgaca gaggtgaacg aacgcagctg ccgcacctgt
    14281 aagccaaaag acgacgagta aaatgccagg ttttactatg gatacgaaaa aactattcaa
    14341 gcacataccc tgggtgattc tcggaatcat cggtgcattc tgcctcgcgg tagttgcatt
    14401 acgtcggggg gagcacgtca gcgccctgtg gatcgtggtc gcctctgtgt cggtgtatct
    14461 ggtggcgtat cgctactaca gtctgtacat cgcccagaag gtgatgaaac tcgaccccac
    14521 gcgcgcgacg cctgcggtta ttaacaacga cggtctgaac tacgttccga ccaaccgtta
    14581 cgtgttgttt ggtcaccact tcgccgctat cgccggtgcc ggtccgctgg ttggtccggt
    14641 gctcgccgcg cagatgggct acctgcctgg cacgctgtgg ctgctggcgg gggtagtact
    14701 ggccggtgcg gttcaggact ttatggtgct gtttatctcc tctcgccgta acggcgcatc
    14761 tctcggtgag atgatcaaag aagagatggg accagtaccg gggactatcg cgctgtttgg
    14821 ctgtttctta atcatgatca tcatcctcgc cgttctggcg ctgattgtgg ttaaagccct
    14881 ggccgaaagt ccgtggggtg tcttcaccgt ttgctcaacc gtaccgattg cgctgtttat
    14941 gggtatctac atgcgcttta tccgtccggg gcgtgtaggt gaagtttctg ttattggtat
    15001 cgtgctgctg gttgcctcta tctatttcgg tggcgtgatt gctcacgatc cgtactgggg
    15061 tccggcactg acctttaaag acaccaccat taccttcgcg ctgattggct atgcgtttgt
    15121 ttccgcactg ctgccggtgt ggctgatcct cgcaccgcgc gactatctgg caaccttcct
    15181 gaaaatcggc gttatcgtcg gcctggcgct gggtatcgtg gtactgaacc cggaactgaa
    15241 aatgcctgca atgacccagt acattgacgg tactggcccg ctgtggaaag gcgctctgtt
    15301 cccgttcctg ttcatcacca tcgcctgtgg tgcggtatct ggcttccacg cgctgatctc
    15361 ttccggtacg acgccgaaat tgctggctaa cgaaaccgac gcgcgtttca ttggctacgg
    15421 cgcaatgcta atggaatcct tcgtggcgat tatggcgctg gttgctgcgt ccatcatcga
    15481 accgggtctt tacttcgcga tgaatacccc gcctgctggc cttggcatca ccatgcctaa
    15541 cctgcatgaa atgggtggcg agaacgcgcc gatcatcatg gcgcagctga aagacgttac
    15601 cgcacacgcg gcggcgaccg tcagctcctg gggcttcgtg atttcgccag agcagatcct
    15661 gcaaaccgcg aaagacattg gtgagccttc tgtcctgaac cgtgcaggtg gcgcgccgac
    15721 gctggcggta ggtatcgctc acgtgttcca caaagtgctg ccgatggctg acatgggctt
    15781 ctggtatcac ttcggtattc tgttcgaagc cctgttcatc ctgaccgcgc tggatgcggg
    15841 tactcgttct ggccgcttta tgctgcaaga cctgctgggt aacttcatcc cgttcctgaa
    15901 aaaaaccgac tctctggttg ccggtatcat cggtactgcg ggctgtgtgg gtctgtgggg
    15961 ctacctgctg tatcagggcg tggtcgatcc gctgggcggc gttaagagcc tgtggccgct
    16021 gttcggtatt tccaaccaga tgctggcagc cgtagcgctg gtactgggca ccgttgtgct
    16081 gattaagatg aagcgcaccc aatacatctg ggtaactgtt gttccggctg tatggctgct
    16141 tatctgcacc acctgggcgc tgggtctgaa actgttcagc accaacccgc agatggaagg
    16201 cttcttctac atggcaagcc agtacaaaga gaagattgct aacggtactg acctgacggc
    16261 gcagcagatt gccaacatga accacatcgt tgtgaacaac tacaccaacg caggcctgag
    16321 tattctgttc ctgattgtgg tgtacagcat catcttctac ggtttcaaaa cctggcttgc
    16381 ggtgcgtaac agcgacaaac gtactgacaa agaaactccg tacgttccaa tcccggaagg
    16441 cggcgtgaag atctcttcgc accactaacc ggttgccgga tgcgctgcgc gtatccggcc
    16501 tacaaagtgt ctatcgtagg ccggataagg cgtttacgcc gcatccggca aatccctagc
    16561 ccggaccttc cgggctttac gcatatctgg aaggcaaaat gtttggtaac ttaggacagg
    16621 caaaaaaata tctcggccag gcggcgaaga tgttgattgg cattccagac tacgacaact
    16681 acgtcgagca tatgaagacc aaccatcccg acaagccgta catgagctat gaagaattct
    16741 tccgcgaacg ccagaacgcg cgctacggcg gcgatggtaa aggcggtatg cgctgttgtt
    16801 aatggagaga gcatgaaccc gattgcagtt accctactca ccggtttttt aggcgcgggc
    16861 aaaaccaccc tgctgcgcca tattcttaac gaacaacacg gctacaagat tgccgtgatt
    16921 gaaaacgaat tcggcgaagt ctccgttgat gatcaactga ttggcgaccg cgccacgcag
    16981 atcaaaacgc tgaccaacgg ctgcatctgc tgttcgcgct ccaacgagct ggaagacgca
    17041 ctactcgacc tgctggataa tctcgacaag ggcaatattc agttcgaccg tctggtcatt
    17101 gaatgcaccg gcatggccga tcccggcccg attattcaga ccttcttctc ccatgagatt
    17161 ttatgccagc gctacctgct ggacggggtg attgcgctgg tggatgcggt acatgccgat
    17221 gagcagatga accagttcac catcgcccag tcccaggttg gctacgccga ccgtattctg
    17281 ctgacgaaaa ccgacgtcgc aggcgaagca gagaaactgc gtgaacgcct ggcgcgcatc
    17341 aacgcccgcg caccggtcta caccgttacc cacggcgata tcgacctggg ccttctgttc
    17401 aacaccaacg gttttatgct ggaagaaaac gtcgtcagca ccaaaccgcg tttccacttt
    17461 atcgcggaca aacaaaacga tatttcgtcg attgtggtgg aactggatta cccggtagat
    17521 atcagcgaag tttcccgggt gatggaaaac ctgctgctgg aatcggcgga taaactgctg
    17581 cgttacaaag ggatgctgtg gattgacggc gaacctaacc gcctgctgtt ccagggcgtc
    17641 cagcgcctct acagcgccga ctgggaccgc ccgtggggcg atgaaaaacc gcatagcacg
    17701 atggtgttta tcgggattca gttgccggag gatgagattc gggcggcgtt tgcgggactc
    17761 aaaaagtaac cagccctcca acatcacaat acctgagact ttatcaccct catattcctc
    17821 cagaatatga gggcattcgg gcttaattat ctctgaacgt gcaacacttc ctacgactcc
    17881 ttatgcgcct ccacatcctg cttcgttaac cagagcgact tacgaccaaa attttcgtac
    17941 attttcagct gatcttcata gtgctgatca actgttccat cgggagcaat aaacccactc
    18001 tgaccgggtg cgaccacatc ccaggcaagc accggacgat cgcttgtcgt tggtgagaaa
    18061 acaatcatat cgttttctgt tccacggttt tgatactccg cctgatgacg cgtttcttcc
    18121 gctgcggcct gcggtacatc aaagaaatta tttgcccgga acgttaaggc cattgcaggt
    18181 gttttccagt tactcacatt attgccatag cgtttggaaa gagtctccca ggtatcttcc
    18241 agcgcagcca acacaacctc ctgctgtggt ttcccagcaa acagatcaac cgcctgtggg
    18301 attggtgatt tgtctccctg caccgcctca tacaaaattt ttgctccaac acttatattc
    18361 agcgaaccag ttgggccgtc ctgggttgtt tcgtagccac tggcgctgta ccacttatca
    18421 aatggcatag gtacggcagc cactacggta cgcttcaaca tactggtcag ccaaacgttc
    18481 aggatggcag agcctggc
//
LOCUS       DAJVUJ010000079        18345 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.079, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000079 DAJVUJ010000000
VERSION     DAJVUJ010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18345
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.079"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(<1..311)
                     /locus_tag="OQQ48_002401"
     CDS             complement(<1..311)
                     /locus_tag="OQQ48_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HCS1953145.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     G"
     gene            complement(316..501)
                     /locus_tag="OQQ48_002402"
     CDS             complement(316..501)
                     /locus_tag="OQQ48_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HCS1953146.1"
                     /translation="MKILLAALTSSFMLAACTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLY"
     gene            complement(498..3137)
                     /locus_tag="OQQ48_002403"
     CDS             complement(498..3137)
                     /locus_tag="OQQ48_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HCS1953147.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVIAFEYQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALISGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTNINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3345..4334
                     /gene="ldhA"
                     /locus_tag="OQQ48_002404"
     CDS             3345..4334
                     /gene="ldhA"
                     /locus_tag="OQQ48_002404"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCS1953148.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPANYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4445..4867
                     /gene="hslJ"
                     /locus_tag="OQQ48_002405"
     CDS             4445..4867
                     /gene="hslJ"
                     /locus_tag="OQQ48_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HCS1953149.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(4864..5130)
                     /locus_tag="OQQ48_002406"
     CDS             complement(4864..5130)
                     /locus_tag="OQQ48_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HCS1953150.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            5404..8928
                     /gene="nifJ"
                     /locus_tag="OQQ48_002407"
     CDS             5404..8928
                     /gene="nifJ"
                     /locus_tag="OQQ48_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HCS1953151.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DSYFQSREATNPWYNAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQTLPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGKRETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTQPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVEGNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
     gene            9294..10427
                     /gene="ompN"
                     /locus_tag="OQQ48_002408"
     CDS             9294..10427
                     /gene="ompN"
                     /locus_tag="OQQ48_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HCS1953152.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGFSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            10568..11002
                     /gene="uspF"
                     /locus_tag="OQQ48_002409"
     CDS             10568..11002
                     /gene="uspF"
                     /locus_tag="OQQ48_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HCS1953153.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            11559..12473
                     /gene="sitA"
                     /locus_tag="OQQ48_002410"
     CDS             11559..12473
                     /gene="sitA"
                     /locus_tag="OQQ48_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HCS1953154.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            12473..13300
                     /gene="sitB"
                     /locus_tag="OQQ48_002411"
     CDS             12473..13300
                     /gene="sitB"
                     /locus_tag="OQQ48_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HCS1953155.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGYMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
     gene            13297..14154
                     /gene="sitC"
                     /locus_tag="OQQ48_002412"
     CDS             13297..14154
                     /gene="sitC"
                     /locus_tag="OQQ48_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HCS1953156.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            14151..15008
                     /gene="sitD"
                     /locus_tag="OQQ48_002413"
     CDS             14151..15008
                     /gene="sitD"
                     /locus_tag="OQQ48_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HCS1953157.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(15336..16109)
                     /locus_tag="OQQ48_002414"
                     /pseudo
     CDS             complement(15336..16109)
                     /locus_tag="OQQ48_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISSfl3 family transposase"
     gene            complement(16115..16627)
                     /locus_tag="OQQ48_002415"
                     /pseudo
     CDS             complement(16115..16627)
                     /locus_tag="OQQ48_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309983.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
     gene            complement(16666..16953)
                     /locus_tag="OQQ48_002416"
     CDS             complement(16666..16953)
                     /locus_tag="OQQ48_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1953158.1"
                     /translation="MKIEELREIFSENGLYAVRVENGAIVSHCRIRCLQSQQRKSGAV
                     LFYFCNGLLTDGFILREDEFVTSLRVLKEIGFKAGFLLLLKNKLIYNLEQD"
     gene            complement(17203..17358)
                     /gene="hokD"
                     /locus_tag="OQQ48_002417"
     CDS             complement(17203..17358)
                     /gene="hokD"
                     /locus_tag="OQQ48_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCS1953159.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESRK"
     gene            complement(17586..18251)
                     /locus_tag="OQQ48_002418"
     CDS             complement(17586..18251)
                     /locus_tag="OQQ48_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HCS1953160.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASGIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
BASE COUNT         4561 a         4676 c         4399 g         4709 t
ORIGIN      
        1 ttaccgttta tcccttgtac atattccccc ggtttggccc gagctacgag cttttgcccg
       61 gcgagtttcg cgatatcatc tacagacaca ttattttgct ttgccagttg ttggtaactc
      121 gctttacgtt cggcattaat gtcggttact aatttctctg tttcagcatc cgttttcagc
      181 gcaaccagat aaccataaaa tgtttccccg acccgcccct gcgttctggc ttcatccaga
      241 gtcaatgcca ttacattgct gcttaccagc ccaacaagaa acgcacaaag aagtaatgtt
      301 ttcttcatca tcgcctcagt aaagatcgct acgagtttca agcagctctt cgacatcttt
      361 gtctgccttg atgatgatct catgctcaat tttaacgttc atattgatag tgatcggttc
      421 cttgggcgca gcgacttcaa tacgcggcgt acaggcagcc agcataaaag atgacgtcaa
      481 cgcagccagt aaaattttca ttgtttttcc tcacactctt ttccttgcgg acagtcattt
      541 cccggcagac gtgcgttctg ctccaaccat gcctggagat tatcgccaaa gcgtaaactg
      601 cgccacagcg taaaaatatt ctcttcatga tggtaattaa ggtttaccgt accgctttta
      661 ccatcaacgc gactggtacc tgtaatgttg gcctgcatgg ttaataaacc gagattatct
      721 aaattaatat ttgtcgatga acggctaatt tccatatagc gcaaccagtt aattgctgaa
      781 cccgcagtca tattgtcttt caccaccgca tctgcggtgt ctttatccag ccgcaaggtc
      841 attggcccgc tatttgccag ccaaccatct ttcactatcc atttttcgtt gtttagccat
      901 aacggcaatg caccactaaa tgccccggac atggcgaatt gtttcggatt gacggcgcta
      961 accagttcgc tggatgagag attattcagc cgcaacagcg ccgggtcatg ttgcggcata
     1021 cgtaattgtt tcatcagtac gttaccgcct aacacatcga cgctaacatc agtcaacagc
     1081 aagggttctt cttcagtcca cggataacgc ccttgcaaat cagccgtaat atttttcgct
     1141 gtcaccagat taatcacttc ggcaattcgc aacgtaacgg ggccgcgagt ccccagatgc
     1201 caggctccat cggcaaaacg gaaaggcagg acaaaatcga caccgttaac ctgattatct
     1261 ggcatccagg cactaccgcc tttcaacacg ccgtgccctc ccgcgcggaa tccttgttca
     1321 ggcgcagcag aaaatgcaac ctgagcatac agttcaccat cgcgtaagtt catcttccag
     1381 tcgggtggca ccagcggctg gaatacagtc agtgattgtt ttggccacca ggcgttaccg
     1441 cgcagacgaa taccgtccca gcgaccattt acccgaaccg ggccaatctc accagcatgt
     1501 aaatcgcctt taaagaggaa atacgtcgga tcgcgcccat cgacgctaaa ttttaaggtt
     1561 gatggcggta acacactgcc gccagtgaac agcgtttgcc cggcgtccag tgacagcgcg
     1621 ccgctgaagg agggatgctg tgcgtcacgt acccagacga cgggcttgtc cagaattaat
     1681 cgcggctttt ctaccgtcat cgtaccgtat tgtaactggt cgaaaccggt ggagaggtcg
     1741 gtcagcgtaa tcgtgctgtc atgccattca ccttttcctg cgacatccca ggtggcattc
     1801 atcggtgtaa aactgccttt tccccagtag cgccactgcc agcggcccgc gtcagggaga
     1861 aaatcattcg ccagcccatc catatgcagc acgaaatcgc ccagttcatt ttcatgcgcc
     1921 tgcaaaatgg cctgcaaacg tccgtcaaca ccacgttggg tgacttttac acccgctaaa
     1981 ggccagcgga tttcatcgat gtccagcgaa tcgatgactc ttccctttga acgaagtaac
     2041 gcgcctggct caaaggccag cgttgggtca gtcagacttc cacttagctg cgcgggtaag
     2101 cgggcatata aaatgaggtc cgcctgtttc gcttcaccgg tcagctgcag aggcagctgg
     2161 ctgttatcca tgcttaattt tcctgggcca aaatttagca ccgcgttgcc tttacccgct
     2221 tgcccctggg tcagcacact cagtcgtccg ctgatcagag cgttctcaag ccctgcctgc
     2281 cagttgtcga ctttgacacc cagtcggcca ctcaaaggaa aacctgcata cggccaactc
     2341 cagcgaccat cgcttacggt caattgttga cgagtaattt gccacggcaa atcgagcaac
     2401 ggatcgccgt tatcacgtgc cagcacaatc aattgcccgc tattttcctg ccagtccagc
     2461 tctgcatcca ccagtgacgg ttcctgcggc aagtttaacg tcgcagtagc atgcccactt
     2521 acaggaagtc catccggcac gagcggcata gtaaattccc ccaccagttt aaccggcggc
     2581 tgatattcaa acgcgatgac atccagttcg ctgactgtaa gttgttgccc tttcagctgg
     2641 ccttgaaatt taactttttc gccctgataa cgcagttgct ggatatcaga ggttaatgcg
     2701 agagagagtt ttccctgcca ttcctgccag ggagaaaaaa tcagtttatc gatattgatc
     2761 caggtgttag gcagcatgga ctgccactgc gcgagggttt ttggagcagc tggcgattgc
     2821 tccgtctgcg gcaatttcgc cagacaagca gaatcaagtt ctaccgtgcc gacgttcaat
     2881 aaccagcggc tgggatgtga aaggctggcg ttggtgatat gtgcaagctg acaatctccc
     2941 accagataac ggagatcggg aatgattaaa cctttacgcg taatgcgtgg gctttcatct
     3001 aatgcaatac gtgtcccgag cggtagccag atgcccgcca gcgtgggaac ccacagcccg
     3061 agcgtcatca gcagcgtcaa cggcacaaga ataatcagta ataacagcgc gagaacggct
     3121 ttatatttac ccagcatggg tagttaatat cctgatttag cgaaaaatta agcattcaat
     3181 acgggtattg tggcatgttt aaccgttcag ttgaaggttg cgcctacact aagcatagtt
     3241 gttgatgaat ttttcaatat cgccatggtt ttcaattata tttgaaattt tgtaaaatat
     3301 ttttagtggc ttaaatgtga ttcaacatca ctggagaaag tcttatgaaa ctcgccgttt
     3361 atagcacaaa acagtacgac aagaagtacc tgcaacaggt gaacgagtcc tttggctttg
     3421 agctggaatt ttttgacttt ctgctgacgg aaaaaaccgc taaaactgcc aatggctgcg
     3481 aagcggtatg tattttcgta aacgatgacg gcagccgccc ggtgctggaa gagctgaaaa
     3541 agcacggcgt taaatatatc gccctgcgct gtgccggttt caataacgtc gaccttgatg
     3601 cggcaaaaga actggggctg aaagtagtcc gtgttccagc ctatgatcca gaggccgttg
     3661 ctgaacacgc catcggtatg atgatgacgc tgaaccgccg tattcaccgc gcgtatcagc
     3721 gtacccgtga cgctaacttc tctctggaag gtctgaccgg ctttactatg tatggcaaaa
     3781 cggcaggcgt tatcggtacc ggtaaaatcg gtgtggcgat gctgcgcatt ctgaaaggtt
     3841 ttggtatgcg tctgctggcg ttcgatccgt atccaagtgc agcggcgctg gaactcggtg
     3901 tggagtatgt cgatctgcca accctgttct ctgaatcaga cgttatctct ctgcactgcc
     3961 cgctgacacc ggcaaactac catctgttga acgaagccgc cttcgatcag atgaaaaatg
     4021 gcgtgatgat cgtcaatacc agtcgcggtg cattgattga ttctcaggca gcaattgaag
     4081 cgctgaaaaa tcagaaaatt ggttcgttgg gtatggacgt gtatgagaac gaacgcgatc
     4141 tgttctttga agataaatcc aacgacgtga tccaggatga cgtattccgt cgcctgtctg
     4201 cctgccacaa cgtgctgttt accgggcacc aggcattcct gacagcagaa gctctgacca
     4261 gtatttctca gactacgctg caaaacttaa gcaatctgga aaaaggcgaa acctgcccga
     4321 acgaactggt ttaatgttgc agctcccctg caatccaggg gagctgattc agataatccc
     4381 caatgacctt tcattctcta ttcttaaaat agtcctgagt cagaaactgt aattgagaac
     4441 cacaatgaag aaagtagccg cgtttgttgc gctaagcctg ctgatggcgg gatgtgtaag
     4501 taatgacaaa attgctgtta cgccagaaca gctacagcat catcgctttg tgctggaaag
     4561 cgtaaacggt aagcccgtga ccagcgataa aaatccgcca gaaatcagtt ttggtgaaaa
     4621 aatgatgatt tccggcagca tgtgtaaccg ctttagcggt gaaggcaaac tgtctaatgg
     4681 tgaactgaca gccaaagggc tggcaatgac ccgtatgatg tgcgctaacc cgcagcttaa
     4741 tgaactcgat aacaccatta gcgaaatgct gaaagaaggt gcacaagtgg atctgaccgc
     4801 gaaccagtta acgctggcga ccgcgaaaca gacattaact tataagctgg cggatttaat
     4861 gaattaatag ctgccacagc tcccggcggc aagtgactgt tcgctacagc gtttgccgtt
     4921 gggtaatgca cacatcccaa tcgccgtgcc atccagttga cgggcaacag aaagcgaacc
     4981 gccgatcatt gcacaatttg cttctccact actggacatc gacgctttta aacctggcgc
     5041 tacgtgcgcc gcagtcgcct gctgaacagg ttcactacta cacgccgaca acaataaagc
     5101 ggcacaccct acccaaaacg ctgctcgcat cttttcttcc tctgatcttc aagccaaacg
     5161 acaccgccat aaataatagg cagcacagag ggcgacatcg agagctgtcc tgcgcgttgc
     5221 cccgcatttt tactttttta tggctatttt tttgccctct gtttgatcaa aacattcatt
     5281 acgctgatgt gggggacaca aaagcgaaaa tgcagaagaa agccatttgc taaaattgaa
     5341 agattactac tgggcgcgca gcaatttcgt gcgcccctca tttgcgcaat gtaagggtgt
     5401 catatgatta ctattgacgg taatggcgcg gttgcttcgg tggcgtttcg caccagtgaa
     5461 gttatcgcca tctaccctat tacccccagt tccacgatgg cagaacaggc tgatgcctgg
     5521 gccggaaacg gcttgaagaa cgtttgggga gacacaccac gcgtggttga aatgcagtcg
     5581 gaagcgggtg ctatcgctac cgtgcatggc gctttgcaga cgggtgccct ttcaacatcg
     5641 tttacgtcat cgcagggttt gctgctgatg atcccgacac tgtacaaact ggcaggcgaa
     5701 ctcacaccgt ttgtcctgca tgtagcggca cgtaccgttg ccacacatgc actctctatt
     5761 tttggcgatc attccgacgt catggcggtg cgccagacgg gttgcgcgat gttgtgtgcg
     5821 gcaaacgtcc aggaagcgca agactttgct ctcatttcgc acatcgcgac gctgaaaagc
     5881 cgcgtgccat ttattcattt ctttgatggt ttccgcacgt cccacgaaat caataaaatt
     5941 gtcccgctgg ccgatgacac gattcttgat ctcatgccgc aggccgaaat tgatgctcat
     6001 cgcgcccggg cgctcaaccc ggaacatccg gtgatccgtg gtacgtccgc caatcctgac
     6061 tcttatttcc agtctcgcga agccaccaac ccctggtaca acgcagtcta tgaccatgtt
     6121 gaacaggcga tgaatgattt ctctgccgcg acaggtcgtc agtatcagcc gtttgaatat
     6181 tacgggcatc cgcaagctga acgggtgatt atcctgatgg gctcagccat tggcacctgt
     6241 gaagaagtgg ttgatgaatt gctaacccgt ggcgaaaaag tcggcgtgct gaaagttcgc
     6301 ctgtaccgcc ccttctcagc caaacacttg ctacaaactc taccgggatc cgtacgcagc
     6361 gtggcggtac tggacagaac caaagaaccc ggtgcccagg cagaaccgct ctatctggat
     6421 gtgatgaccg cactggcaga agcctttaat aatggcaagc gcgaaactct gccccgtgtc
     6481 attggtgggc gctatggtct ttcatccaaa gaatttggcc cggactgtgt actggcggta
     6541 tttgccgagc tcaacgcggc taaaccgaaa gcgcgcttta cggttggtat ttacgatgat
     6601 gtaaccaatc tgtccctgcc gttgccggaa aacacccagc caaactcggc gaaacttgaa
     6661 gccttgtttt atggccttgg tagtgatggc agcgtttccg cgaccaaaaa caatatcaag
     6721 attatcggta attccacgcc gtggtacgca cagggctatt ttgtttacga ctccaaaaaa
     6781 gcgggcggcc tgacggtttc tcaccttcgc gtgagcgaac agccgattcg ttccgcttat
     6841 ctcatttccc aggctgattt tgttggctgc caccagttgc agtttatcga taaatatcag
     6901 atggctgagc gtttaaaacc tggcggcatt ttcctgctca acacgccgta cagcgcagat
     6961 gaagtgtggt cgcgcttgcc gcaagaagtt caggccgtgt taaaccagaa aaaagcgcgc
     7021 ttctatgtga ttaacgcggc gaaaatcgcc cgcgaatgtg gcctggcggc ccgtattaat
     7081 accgtcatgc agatggcttt tttccatctg acgcaaattc tgcctggcga tagcgccctc
     7141 gcagaattgc agggtgcgat tgccaaaagt tacagtagca aaggccagga tctggtggaa
     7201 ggcaactggc aggctctggc gctggcgcgt gaatccgtag aagaagttcc gttgcaaccg
     7261 gtaaatccgc acagcgccaa tcgaccgcca gtggtttccg atgccgcccc tgatttcgtg
     7321 aaaaccgtaa ccgcagcgat gctcgcaggg cttggtgatg ccctccccgt ttcggcgctg
     7381 ccgccagacg gcacctggcc gatggggacc actcgctggg aaaaacgcaa tatcgccgaa
     7441 gagatcccca tctggaaaga ggaactctgt acccaatgta accactgcgt cgccgcttgc
     7501 ccacactcag ctattcgcgc aaaagtggta ccacccgaag cgatggaaaa cgcccctgcc
     7561 agcctgcatt cgctggatgt gaaatcgcgt gatatgcgcg ggcagaaata tgtcttgcag
     7621 gtggctccgg aagattgcac cggttgtaac ctgtgcgtcg aagtttgccc ggcgaaagac
     7681 cgtcagaatc cagagattaa agccatcaat atgatgtcgc gcctggaaca tgtcgaagaa
     7741 gagaaaatca attacgattt cttcctcaac ctgccagaaa tcgaccgtag caaactggaa
     7801 cgtattgata ttcgtacatc gcagctgatt acaccgctgt ttgaatattc cggtgcttgc
     7861 tccggttgtg gcgagacgcc gtatattaaa ttgctgactc agctctatgg cgaccggatg
     7921 ttgatcgcta acgccaccgg ctgttcttca atttatggcg gtaacctgcc ctctacaccg
     7981 tataccaccg atgccaacgg tcgtgggccg gcatgggcga actctctatt tgaagataat
     8041 gccgaatttg gccttggttt ccgcctgacg gtcgatcaac accgtgtccg cgtgctgcgt
     8101 ctgctggatc aatttgccga taaaatcccg gcggaattac tgacggcgtt gaaatcagac
     8161 gctacgccag aggttcgtcg agaacaggtc gcagctttac gccagcaact caacgatgtt
     8221 gccgaagcac atgaactgct acgtgatgca gatgcactgg tggaaaaatc aatctggctg
     8281 attggtggtg atggctgggc ttacgatatc ggctttggcg gtctggatca tgtattgagt
     8341 ttgacggaaa acgtcaacat tctggtgctg gatacgcaat gctattccaa caccggtggt
     8401 caggcgtcga aagcgacacc gctgggtgca gtaactaaat ttggcgagca cggcaaacgt
     8461 aaagcgcgta aagatcttgg cgtcagtatg atgatgtacg gtcatgttta tgtggcgcag
     8521 atttctctcg gcgcgcagct gaatcagacg gtgaaagcga ttcaggaagc ggaagcgtat
     8581 ccgggaccat cgctgatcat tgcttatagc ccgtgtgaag agcatggtta cgatctggca
     8641 ctcagccatg accagatgcg ccaactcaca gctaccggct tctggccgct atatcgcttc
     8701 gatcctcgtc gtgccgatga aggcaaactg ccgctggcgc tggattcacg cccgccgtca
     8761 gaagcactgg aagaaacgtt acttcacgag caacgtttcc gtcgtctgaa ttcgcagcag
     8821 ccagaagtgg cagaacagtt atggaaagat gctgcagctg atttgcaaaa acgctatgac
     8881 ttcctggcac aaatggccgg aaaagcggaa aaaagcaaca ccgattaaat gctctggata
     8941 aggattatcc aattctaaaa aaaagcccgg acgactgttc gggcttgtct ttttatatgt
     9001 tggaaaatca gtggcaatgc aatggcccaa cagaaaataa aatctgtgac aataaaggca
     9061 tataacccgc gcagaataac gtatagaaaa ttaaaagtat aatttttatt ttgtatgtat
     9121 tatcttcgat acattcacca gacttattat catttcagaa gaattatttg attaaggttt
     9181 tacttaaggc gtaacaaatg atatttatcg gctaactgaa cttctccttt aggatgtttt
     9241 cactccccgg aatggggata aaaggcaaat aaaataacta aaggatttat tcaatgaaaa
     9301 gcaaagtact ggcactttta attcctgccc tgctcgccgc aggtgctgca catgcagccg
     9361 aagtttataa taaagacggc aacaaattag atctgtatgg caaagttgat ggcctgcatt
     9421 atttttctga taattcagcg aaagatggcg atcagagcta tgcgcgtctg ggttttaaag
     9481 gcgaaaccca aattaacgat caactcactg gctacggtca atgggaatac aatattcaag
     9541 caaacaacac tgaatcttca aaaaaccagt catggacccg tctggcattt gccgggctga
     9601 aatttgcaga ttacggttct ttcgattacg gacgtaatta tggcgtaatg tacgacatcg
     9661 aaggctggac cgatatgctg cctgaatttg gcggtgactc ttataccaat gcagacaact
     9721 ttatgactgg tcgagccaat ggcgtcgcga cttatcgtaa tactgatttc ttcggtctgg
     9781 taaatggtct gaacttcgcg gtgcagtatc aaggtaacaa cgaaggagcc agtaatggtc
     9841 aggaaggcac caacaacgga cgtgatgttc gccatgaaaa cggtgacggc tggggttttt
     9901 ccacaacata tgatttaggc atgggcttta gcgctggtgc ggcatacacc tcttctgacc
     9961 gcaccaatga ccaggttaac catactgcgg cgggtggtga taaagcagac gcgtggactg
    10021 ctgggctaaa atacgatgct aacaatattt acctggcaac catgtattca gaaacgcgta
    10081 atatgacccc gtttggcgac agcgattatg ctgtggcaaa caaaacccag aattttgaag
    10141 tcactgccca gtaccagttt gattttggtc tgcgtccggc agtctctttc ctgatgtcta
    10201 aaggccgtga cctgcacgct gccggtggtg cagacaaccc ggcaggtgtt gatgataaag
    10261 atctggttaa atacgccgat gttggcgcga cttactattt caataaaaac atgtccacct
    10321 acgttgacta taaaatcaac ctgttggatg aagatgacag cttctacgct gccaatggca
    10381 tctctaccga tgatattgtc gctttaggtc tggtttatca gttctaaatc ctcctgcccg
    10441 ctgttatggc gggctttttc tgcttattct tcctcctttg atctaaatta aaaatgtgaa
    10501 ctccatcatt acacaaaaag tgtcatctgg cgttacactt tatgcggata ctaaaacagg
    10561 aggttttatg aacagaacga ttcttgtccc tatcgatatt tccgattcag aattaactca
    10621 acgcgtgatt agccacgttg aggcagaggc aaagattgac gatgcagagg ttcatttcct
    10681 gacggtaata ccttcactgc cctactatgc ctctctgggt ttagcgtatt ccgcagaatt
    10741 accggcaatg gatgacctga aagcggaagc caaatcgcaa ctggaagaga tcattaaaaa
    10801 atttaaactg ccaaccgaca gagtgcatgt ccatgttgag gaaggctcgc ccaaagaccg
    10861 cattctggaa ttggcgaaga agatccccgc tcatatgatc atcattgctt cccatcgacc
    10921 ggatatcacc acttatctgc tcggttccaa cgccgcagct gtagtgcgtc acgcagagtg
    10981 ctccgtgctg gttgtgcgct gacactaacg cccgcacatt gctgcgggct ttttgattca
    11041 tttcgcaaat gtgctgacat tttcccctct aatccgtacc atacacgcca ctgtttttat
    11101 atcagatttc ttatgctggg cattccggca tgacgcatac tcttctgatg ccatataacg
    11161 aattgagtcg cttttaaatg tcgcaaaatc aagaaattag taagaaagaa caatacaacc
    11221 tgaacaagta agggcaaaaa tcacaactat ctgactcata agtattttat ttatttttca
    11281 atatgttaaa cattatcagc gacaacaata agctacgata acccactctt tttttgcccc
    11341 atttttgcca tcaacaattt tcctcataaa gtcatttcaa agcctaatag ctgctctact
    11401 tttttcctca atatcatgtc gccagcagga aacaacctaa tccctgtttt aggcctcaaa
    11461 gctgttaatg ataattattt tcattttgta taggttatgc tataaaacat agccgttgct
    11521 atgtttgttc taattttgct cactataggt actaaattat gcactcaata aaaaaagtaa
    11581 ccatgctctt ggggtgcctc gccctcacct gctcgatcgc atttcaggca agtgcaactg
    11641 aaaaattcaa ggtcattaca acatttacca tcatcgcgga tatggccaaa aacgtggctg
    11701 gagatgctgc agaagtctca tccataacca agcctggtgc agaaattcat gagtatcagc
    11761 ctacccctgg cgatattaaa cgtgcacagg gggcacaact gattctcgcc aatggtatga
    11821 atctggaatt gtggttccaa cgcttttacc agcatctcaa tggggttcca gaagtaattg
    11881 tctcttcggg tgtgacgcca gtagggatca ccgaaggacc ctatgagggc aaacctaatc
    11941 cccatgcatg gatgtcgcca gataatgctc tgatttacgt cgataatatt cgtgatgcgt
    12001 tgataaaata cgatccggca aatgcacaaa cctaccaacg taatgccgat acttataaag
    12061 ccaagattac ccaaaccctt gcccccctgc gtaagcagat tacggaactc cctgagaatc
    12121 agcgatggat ggtcaccagt gaaggggctt tttcttatct cgcacgcgat ttggggctaa
    12181 aagagcttta tctgtggccg attaatgccg atcaacaagg aacaccgcag caggtacgta
    12241 aggttgttga tatagttaag aaaaatcata tcccggcagt ctttagcgag agtacgattt
    12301 ccgataaacc agcgcgtcag gttgcgcgtg aaaccggcgc acactacggc ggtgtgctct
    12361 atgtcgattc cctgagcaca gaaaacggcc cggtacctac gtatatcgac cttcttaagg
    12421 ttactaccag tacgctggta cagggaatta aagccgggaa aagggagaaa taatgatgca
    12481 atctgcaggc attgtcgtta atgatgttac tgtcacctgg cgtaacgggc acacagcact
    12541 gcgcgatgca tccttcacag taccaagtgg atctattgcc gctctggttg gggtaaacgg
    12601 ttccggaaaa tcgacgttgt ttaaagcgat tatgggattt gtgcgtctga ccagcggaaa
    12661 gatatctgtt ctgggtattc ccacgcgaca ggcgctacag aaaaacctgg ttgcctacgt
    12721 ccctcagtca gaagaggttg actggtcatt tcctgtactg gtggaagatg tggtgatgat
    12781 gggccgctat gggtatatgg gtatgttgcg tatagccaaa aagagggatc gtcagattgt
    12841 caccgatgcg ctggaacgtg tcgatatggt ggattttcgc catcgtcaaa tcggcgagct
    12901 ttccggtgga caaaagaaac gcgtctttct ggcgagagcg attgcacagc agggtgatgt
    12961 gatcctgctg gatgaacctt ttaccggcgt tgatgtgaaa accgaagcga aaatcatcag
    13021 cctgttgagg gagttacgcg cggaaggcaa aacgatgctt gtctcaacgc acaatctcgg
    13081 ctcagtcacg acattttgcg attatacggt catggtcaaa ggcaccgtcc tggcaagcgg
    13141 gccgacagac accactttta cagccgagaa cctggagctg gcttttagcg gcgtactgcg
    13201 ccatgtcaca cttaacggct cggaagaaag cattattact gacgatgaac gcccttttgt
    13261 ggcacatcga ccgtcagcgg tgcagaggga agcaagatga acgtgctact ggaacccttc
    13321 agctatgaat atatgctcaa tgcgatgtgg gtctcggcaa tggtcggcgg tctctgcgct
    13381 ttcctgtcat gctatctgat gctaaaaggc tggtcgctga ttggcgatgc gctgtcgcac
    13441 tctattgtcc cgggtgttgc gggggcatat atgctggggc ttccgttttc tctgggagcc
    13501 ttcttttccg gcggactggc agcgggcagc atgctgtttc ttaaccaacg cacccgctta
    13561 aaagaagatg ccatcatcgg cctgatcttc tcgtcctttt ttggcctggg gctgtttatg
    13621 gtgtcattga acccgacatc cgtgaacatt cagaccatcg tcctgggcaa tattctggca
    13681 atcgatccgg cggatatcct gcaactcact atcattggca ttctctcgat catcgtgctt
    13741 tttttcaaat ggaaagatct tatggtgacc tttttcgacg agaatcacgc ccgtgctatt
    13801 ggattacatc cgggcagatt aaaaatcctc ttcttcaccc tgctgtcggt ttcgaccgtt
    13861 gcggcgctgc aaacggttgg tgcctttctg gtgatctgtc tggtggtgac tcccggtgca
    13921 acagcatggt tgttaaccga tcgttttccc cgcctgctca taattgctgt aactattggc
    13981 agtgtaacca gtttcctcgg cgcgtgggtc agttactttc tggacggcgc gacaggtgga
    14041 atcatcgtgg tggcgcagac actgctgttt cttttagcat ttgtatttgc gcccacccat
    14101 ggacttttgg caaatcgtcg gcgtgcgcat aaagcgctgg aggatcgctc atgatggcat
    14161 tattactgga gccactgcag ttcaccttta tgagtcacgc actgctgatt tcactggtag
    14221 tttccatacc ctgtgcgctg ctgtcggtct ttctggtcct gaaaggttgg gcactgatgg
    14281 gtgacgcgat gagtcatgcg gtttttcctg gcatcgtgct tacctggatt ctggggttac
    14341 ctctggctac aggggcattc gtcgccggag tattctgcgc ggtggcgacg ggatacctga
    14401 aagacaacag ccgaatcaag caagatacag ttatggggat tgttttttct ggcatgtttg
    14461 ccgcaggtct gatcttgtat attgcaatca aaccagacgt acatcttgac catattcttt
    14521 tcggcgacat gctcgggata accatcggcg atataatcca gacggtgatt attgccgggc
    14581 tggttacact tgttattagc gtaaaatggc gggatttttt gctgttcagc tttgattatc
    14641 aacaggcgca ggtaagcggt ttgcatacga gatggctgca ttatgggctg ctgtgcatgg
    14701 tttccctgac cattgtggcg acgctgaaag cagtaggtat cattctctct atttctctgc
    14761 tcattgcgcc tggcgccatt gcagtactcc tgacacaacg ctttcatatt gcgctcctac
    14821 tggcgactgg catatcggta atagtgtcaa tgactggtgt ctggctgtcc ttttttatcg
    14881 acagcgcacc agctccgacg attgtcgtct tattcgcggt cgtgttcatc atgacgttta
    14941 ccgttaccag catcaacgca cgcacaaagg gaaacgccca tacacaggat ctgttatcac
    15001 ccaattagca caatatccga ggacagcatc gtccgccagc gtccgccagc ctacagaagt
    15061 tcggaaagcc atggaaggtc gttgggagga ggtacgaatt ctatcatgca aaaaatacgt
    15121 aaaatcgata acgcctagaa atcattcaat actcgcacta tcgaaagttt accagccaac
    15181 cgcggcacgt tcctgcatac gacgtgccgc agcttctgtt attccacgat aaaattccta
    15241 accgaattca aatccttcag actattcagc tcttccttca gctcccgctg acggcgataa
    15301 atcgtaagcg ccccgagaag tacgtagcgt aaggattatt ttacagacga gaagttccag
    15361 ggcagcagtt catgcacttg gttcgacggc cagtcattga gcttctcgat cacttcgcgc
    15421 aaccagtcct ccggctccac ttcgttctgt ttgcaggtga ccagcagact gtagatgatc
    15481 gccgcacttt ctcctccctt gtctgagccg aaaaagagat aattttttct tccaaccgcc
    15541 accgatcgta acgcgttttc accgatgttg ttgtctattt ccacccagcc gtcacgacag
    15601 aactcgttca gcgcattcca gtgattcagg atatagtcga acgccttcgc catctccgca
    15661 tgtttcgaca gcgttttcct ctgcaactgt atccagtcgt acaatgactg catcaactgg
    15721 acgcttctgg cttttctgac tgcaagccgt tcctctgccg gactgccacg tatctccgct
    15781 tctatgtcgt ataactctgc tatccgtctg agcgcttcct gagtcatttc tgtcggacgg
    15841 cgcacatcct cgtcatggat ttttcggcgg gcgtgggcca ggcacccggc ttccttcacc
    15901 cggcccgttt cgtacagtac gttataacct gcataggcat cagcctgcag tacgccctga
    15961 tactttgcca ggtggagctg cggatgttct cctttgcgat ctgccgaata cgcgaaccag
    16021 acggctgccg gcagggatga acccgcatta cgatcatccc tgacgtatac ccacagacga
    16081 cccgttttcg tctttccgtt ccccggggcg gttcattcag cacagattta taaacctgct
    16141 gaaactcggt ttcgtccata ttcgcaaacg agatggattt cgccctgcgc ccacggctac
    16201 cgtccggata aatatgctcg gtgtaaaatc cggcctgaat ggttacccac tcgcggaaag
    16261 cctcaaacga ctttagcaat gctgtatctc gggttctgcg tgtcgcaact gtattcagat
    16321 attgctctgc ggcttcgctc agagctggcg tatgttcccg gcctactgat tcgcacaggt
    16381 actcaacgaa gcctgatacc agttctcgtt cgcgaggcgt aatcgcccca ccgatcggag
    16441 tccagtaatc gaatcccagt tgcaggagtt tgaaaaaacg cttgtggaat gcgtagttac
    16501 gcacacgctt aaagtctgcg tgtatccact cacctgtttt gatttgatgc agaaaatcgc
    16561 aactctccgg cgtcgccggg agaagtaatc cggaagaggt ttgtttgatc agttgtatat
    16621 gcgccatcgg ctttctccgg tggcacggtg ttacacagca ggagttcaat cctgctcaag
    16681 attgtagatg agtttattct tcagcaaaag cagaaaacca gccttaaaac caatctcttt
    16741 caaaacccgt aatgatgtga caaattcgtc ctcacgcaaa ataaaaccgt ccgtcagaag
    16801 tccattacaa aaataaaata acacagcacc gctcttcctt tgttgagatt gcaaacatct
    16861 aatgcggcaa tggctgacaa tcgctccatt ctcaacgcgc acagcataga ggccattttc
    16921 actaaaaatt tcacgcaatt cttcgatttt catcttcaga atccttccag ataaatagct
    16981 ctcccctgtt cggggtccat ccctcttctc cctgcgcgct acttaagtga gtcgattcta
    17041 tctgcgaagg tgcgcgaatc aaattcaccg gaaataaaca acaaaaaacc cgccgaaacg
    17101 ctgagagatc acagcgatgg gcgggttaag tgcgggtgcg ttgaggatgc ctgacacatc
    17161 agaggtgggc ggggatggga tcagctcccc gcccggtcac tcttacttcc tggattcgta
    17221 gtctacgaag acagcgacct ccgtctggcc ggttcggatt cgtacctcac agaggtcttt
    17281 cctcgttacc agtgccgtca ctatgacggt taaacagatg acgatcaggg cgattaacat
    17341 cgccttttgc tgcttcatag cctgcttctc cttgcctttc ggcatgtaag aggctaacct
    17401 acatgtgcaa agcatgaaat tggcctcaga ttaatgttaa gcgtcttgcc ggacgcgtaa
    17461 tgttaactgg ggcttttctc tatctgcctt ttggtgttca tgcctgaagc agatagcctc
    17521 aagcacccgc agtcattcta cttacctcgc caatatgaaa tcaatcagaa aggtgcccca
    17581 taaaatcact ccttctcttc tttaccgtag tggagttgac caattttgat aagagggcgt
    17641 ccctgagatt tgcggtgtag attggtatcg cgcagagaat acacacagcc acaatattcc
    17701 tgctgataga atttttcgcg cttgctgatt tcaatcatac gggacgagcc tccctgcttg
    17761 cgccagttat aatcccagta caccatgccc ggataatgcg cgacggctcg ccgcccacag
    17821 tcgttaacct gctgcatatt tttccagcgt gaaatgccca gtgaactgct gatcacactg
    17881 aaaccatttt cagcggcgta cagcgctgtc cgctcaaaac gcatgtcaaa acacatggtg
    17941 caacggatcc ctcgttcggg ctcccattcc attcctttgg ctcgttcaaa ccagttgtct
    18001 gtgtcgtaat cagcatcaat aaacggcacg ccgtgttgtt cagcaaagcg aatattctca
    18061 tccttacgaa ttaaatactc tttctgagga tgaatgttcg ggttgtagaa aaagatggtg
    18121 tagtcgattc ccgaggcctg aagcgcctcc atcacctcac cggaacatgg agcacagcaa
    18181 gagtgcagta gtagtttgtt tgccccgttt gggagctcca atttgggccg tttgaaatca
    18241 gcaactgtca taaatatgtt tattggggtc ataaaaatat caaagagtgt agcattagaa
    18301 cggggctatc ggaaacagat gtgtgactgc tccccgccct ttcgg
//
LOCUS       DAJVUJ010000080        17926 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.080, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000080 DAJVUJ010000000
VERSION     DAJVUJ010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17926
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.080"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            679..1044
                     /locus_tag="OQQ48_002419"
     CDS             679..1044
                     /locus_tag="OQQ48_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCS1953161.1"
                     /translation="MEKCDFYHIIVLSLNFPGYLKMEYGSTKMEERLSRSPGGKLALW
                     AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLGALVGFLFMALVGAL
                     LGSIAWYTRPRPARSRRYE"
     gene            1084..1779
                     /locus_tag="OQQ48_002420"
     CDS             1084..1779
                     /locus_tag="OQQ48_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HCS1953162.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAHDVCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSLAKNHPQRQRSSILAAEETRRLLREEFEQFPAEKIEAVCH
                     VIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPFAQ
                     HRPLDDKRYALDHFQTKLLKLPQTMQTARGKQLAQHNAHFLVEFMAKLSAELAGENEG
                     VDHKVIDAFSPAG"
     gene            1846..3264
                     /gene="dcm"
                     /locus_tag="OQQ48_002421"
     CDS             1846..3264
                     /gene="dcm"
                     /locus_tag="OQQ48_002421"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HCS1953163.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMAKGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            3245..3715
                     /gene="vsr"
                     /locus_tag="OQQ48_002422"
     CDS             3245..3715
                     /gene="vsr"
                     /locus_tag="OQQ48_002422"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HCS1953164.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGSPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDEALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(3704..4624)
                     /gene="yedA"
                     /locus_tag="OQQ48_002423"
     CDS             complement(3704..4624)
                     /gene="yedA"
                     /locus_tag="OQQ48_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HCS1953165.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLMAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            4797..5714
                     /locus_tag="OQQ48_002424"
     CDS             4797..5714
                     /locus_tag="OQQ48_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HCS1953166.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDSLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKIRGQAH"
     gene            5793..5975
                     /locus_tag="OQQ48_002425"
     CDS             5793..5975
                     /locus_tag="OQQ48_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HCS1953167.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            6146..7840
                     /gene="yedQ"
                     /locus_tag="OQQ48_002426"
                     /pseudo
     CDS             6146..7840
                     /gene="yedQ"
                     /locus_tag="OQQ48_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310721.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
     gene            complement(7837..8652)
                     /locus_tag="OQQ48_002427"
     CDS             complement(7837..8652)
                     /locus_tag="OQQ48_002427"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HCS1953168.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLSRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGVPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPARVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(8950..9166)
                     /gene="yodD"
                     /locus_tag="OQQ48_002428"
                     /pseudo
     CDS             complement(8950..9166)
                     /gene="yodD"
                     /locus_tag="OQQ48_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
     gene            9329..9517
                     /gene="dsrB"
                     /locus_tag="OQQ48_002429"
     CDS             9329..9517
                     /gene="dsrB"
                     /locus_tag="OQQ48_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HCS1953169.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            complement(9561..10184)
                     /gene="rcsA"
                     /locus_tag="OQQ48_002430"
     CDS             complement(9561..10184)
                     /gene="rcsA"
                     /locus_tag="OQQ48_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HCS1953170.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQHIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKHKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(10469..11254)
                     /gene="fliR"
                     /locus_tag="OQQ48_002431"
     CDS             complement(10469..11254)
                     /gene="fliR"
                     /locus_tag="OQQ48_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HCS1953171.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFVIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            complement(11263..11532)
                     /gene="fliQ"
                     /locus_tag="OQQ48_002432"
     CDS             complement(11263..11532)
                     /gene="fliQ"
                     /locus_tag="OQQ48_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HCS1953172.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            complement(11542..12278)
                     /gene="fliP"
                     /locus_tag="OQQ48_002433"
                     /pseudo
     CDS             complement(11542..12278)
                     /gene="fliP"
                     /locus_tag="OQQ48_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly.; frameshifted; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
     gene            complement(12278..12643)
                     /gene="fliO"
                     /locus_tag="OQQ48_002434"
     CDS             complement(12278..12643)
                     /gene="fliO"
                     /locus_tag="OQQ48_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HCS1953173.1"
                     /translation="MNNHATVQSSTPVSAAPLLQVSGALIAIIALILAAAWQVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            complement(12646..13059)
                     /gene="fliN"
                     /locus_tag="OQQ48_002435"
     CDS             complement(12646..13059)
                     /gene="fliN"
                     /locus_tag="OQQ48_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HCS1953174.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMHRLSR"
     gene            complement(13056..14060)
                     /gene="fliM"
                     /locus_tag="OQQ48_002436"
     CDS             complement(13056..14060)
                     /gene="fliM"
                     /locus_tag="OQQ48_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HCS1953175.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            complement(14065..14529)
                     /gene="fliL"
                     /locus_tag="OQQ48_002437"
     CDS             complement(14065..14529)
                     /gene="fliL"
                     /locus_tag="OQQ48_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HCS1953176.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALNTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            complement(14634..15761)
                     /gene="fliK"
                     /locus_tag="OQQ48_002438"
     CDS             complement(14634..15761)
                     /gene="fliK"
                     /locus_tag="OQQ48_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HCS1953177.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLISDILADAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADQNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDVPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            complement(15758..16201)
                     /gene="fliJ"
                     /locus_tag="OQQ48_002439"
     CDS             complement(15758..16201)
                     /gene="fliJ"
                     /locus_tag="OQQ48_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
                     /protein_id="HCS1953178.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERLSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            complement(16220..17588)
                     /gene="fliI"
                     /locus_tag="OQQ48_002440"
                     /pseudo
     CDS             complement(16220..17588)
                     /gene="fliI"
                     /locus_tag="OQQ48_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
     gene            complement(17588..>17926)
                     /gene="fliH"
                     /locus_tag="OQQ48_002441"
     CDS             complement(17588..>17926)
                     /gene="fliH"
                     /locus_tag="OQQ48_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HCS1953179.1"
                     /translation="ASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
BASE COUNT         4397 a         4484 c         4717 g         4328 t
ORIGIN      
        1 caaccatctt gccgtagaaa tcaactttat tgccatcttt attgtaaatt tcagccgcat
       61 ttgctgcgcc agcaactaat aacgccggga ccagcattgc cagaactttt cttttcatta
      121 tgtattccct tgtgattata atcttcatga atatatcaat aagtgccgtt atccaaaaaa
      181 aagcacattt ggatactatt ctatgaagtt catttcattt taagtattac atgcaacaaa
      241 tgtatttaaa aggtttcaaa atgtttctaa tctatttaat ataattcaga gatatgaatt
      301 ataaatattc atgaaaatta taaatgtata tcaatatgtt atgagttgag cggaaacata
      361 gggggtatca atgattgttg cgccaaacgg ctggtgtgat caggcgcaca ttaatgaagg
      421 cataaaaaaa agccggcatc atgccggccc aaaaaatata aacaagacga taaaaggcta
      481 ataacggaag catcatgaca cagaataata aaaaggtata attgtaatca gtgctaatat
      541 attgattaaa tgaattaata aagccgatat caaagataaa taaaatcgct caaaacgatt
      601 tttcctgtat tcactgccgt tgcgcaaaat ttatctattt gttcaaaaaa tgattgagtc
      661 ttgactggct catccaatgt ggaaaaatgt gacttttatc acataatagt actaagtctg
      721 aattttccgg gttatctcaa aatggaatac ggttcgacaa agatggaaga gagactctct
      781 cgcagccctg gcggcaaact ggctttgtgg gctttctata catggtgtgg ctatttcgtc
      841 tgggcaatgg cgcgttacat ctgggtgatg agccggatcc ccgatgcccc cgtgtcggga
      901 tttgaaagcg atttaggctc taccgcggga aagtggttag gggcactggt cggattttta
      961 tttatggctc tggtcggggc attgttaggc agcattgcct ggtacacccg acctcgtccc
     1021 gcacgtagca gacgctatga ataaaaactg ctaaggtggc gactctttgg caacggagcg
     1081 cgtatggact tacaacactg gcaggcacag ttcgaaaact ggttgaaaaa ccatcatcag
     1141 caccaggacg cggcccatga cgtgtgtcat tttcgccgcg tctgggctac ggcacagaag
     1201 ctggcggcag atgacgatgt cgatatgctg gtgattttaa ccgcctgtta ttttcacgat
     1261 attgtgagcc tggcgaaaaa tcatccgcaa aggcagcgtt cttcaatcct ggcggcagaa
     1321 gagacgcgtc gcctgctccg tgaagagttc gagcaatttc cggcggagaa aatcgaggcc
     1381 gtttgtcatg tcattgccgc tcacagtttc agcgcgcaaa tcgccccctt aacaacggaa
     1441 gctaaaatcg tccaggatgc ggatcggctg gaagccctgg gggcgattgg cctggcgcgc
     1501 gtgtttgccg tttcaggggc attgggcgtg gcgctgtttg atggtgaaga tccgttcgca
     1561 cagcatcgcc cgcttgatga taaacgctac gcgctggatc atttccagac taagttgctg
     1621 aagctaccgc aaaccatgca aaccgcaaga ggcaagcagc tggcgcagca caacgcgcat
     1681 tttttagtcg agtttatggc gaagctcagt gccgaactgg cgggggagaa tgaaggtgtc
     1741 gatcacaagg tgatagatgc gttttcaccc gcgggataag cgcgtggccc taaatggctg
     1801 taattatgtt aacctgtcgg ccatctcaga tggccggtta aatctatgca ggaaaatata
     1861 tcagtaaccg attcatacag caccgggaat gccgcacagg caatgctgga gaaactgctg
     1921 caaatttatg atgttaaaac gctggtggcg cagcttaatg gtgtgggtga gaatcactgg
     1981 agcgcggcaa ttttaaaacg tgcgctggcg aatgactcgg catggcaccg tttaagtgag
     2041 aaagagttcg cccatctgca aacgttgtta cccaaaccac cggcacatca tccgcattat
     2101 gcgtttcgct ttatcgatct atttgccgga attggcggca tccgtcgcgg ttttgaatcg
     2161 attggcggac aatgcgtgtt taccagcgaa tggaacaaac atgcggtacg cacttataaa
     2221 gccaaccatt attgcgatcc ggcgacgcat cattttaatg aagatatccg cgatatcacc
     2281 ctcagccata aagaaggcgt gagtgatgag gcggcggcgg aacatattcg tcaacacatt
     2341 cctgaacacg atgttttact ggccggtttc ccttgtcagc cattttcgct ggctggcgta
     2401 tcgaaaaaga actcgctcgg gcgggcgcac ggttttgcct gcgataccca gggcacgctg
     2461 ttttttgatg tggtacgcat tatcgacgcg cgtcgtccgg cgatgtttgt gctcgaaaac
     2521 gtcaaaaacc tgaaaagtca cgaccagggt aaaacgttcc gcatcatcat gcagacgctg
     2581 gacgaactgg gctatgacgt ggctgatgca gaagataacg ggccggacga tccgaaaatc
     2641 atcgatggta aacattttct gccgcagcac cgtgaacgca tcgtgctggt gggttttcgt
     2701 cgcgatctta atctgaaagc cgattttact ctgcgtgata tcagcgaatg tttccctgca
     2761 cagcgagtga cgctggcgca gctgttggac ccgatggtcg aggcgaaata tatcctgacg
     2821 ccggtgctgt ggaagtacct ctatcgatat gcgaaaaaac atcaggcgcg cggtaacggc
     2881 ttcggttatg gaatggttta tccgaacaat ccgcaaagcg tcacgcgtac gctgtctgcg
     2941 cgttattaca aagatggcgc ggaaatttta atcgatcgcg gctgggatat ggccaaaggt
     3001 gagaaagact ttgacgatcc gctgaatcag caacatcgtc cacgtcggtt aacgcctcgg
     3061 gaatgcgcgc gcttaatggg ttttgaagcg ccgggagaag cgaaattccg cattccggtt
     3121 tcggacactc aggcctatcg ccagttcggt aactcggtgg tcgtgccggt ctttgccgcg
     3181 gtggcaaaac tgcttgagcc aaaaatcaaa caggcggtgg cgttgcgtca gcaagaggca
     3241 caacatggcc gacgttcacg ataaggccac tcgcagcaaa aatatgcgcg cgattgccac
     3301 gcgtgatacg gcgattgaga agcgcctcgc cagtctgtta accgggcagg gcctggcgtt
     3361 tcgcgttcag gacgccagtc tgcccggaag cccggatttt gtcgttgatg aatatcgctg
     3421 cgtgatattt acccatggct gcttctggca tcatcatcac tgctatctgt ttaaagtgcc
     3481 tgcgactcga accgagttct ggctggagaa gataggtaaa aatgttgaac gcgatcgccg
     3541 cgatatcagt cgtttgcagg aactcggctg gcgcgtactg attgtctggg agtgtgcatt
     3601 acgtgggcgc gagaagctga cggatgaagc gcttaccgag cgtctggaag agtggatctg
     3661 cggcgaaggt gccagcgcgc agatcgacac gcaggggatt catttactcg cttgatgcgt
     3721 cctgaataac tggcgcaact acgggttttg ccgggaagag atattttccc aacgtgacca
     3781 gtaccaccgc gaagacaatt acgccgagcg ccagccattc aatcttcgac agcgtctctc
     3841 cgcccagtcc cgtacccagc aagaccgcga ccaccgggtt aacgtaggcg tagctggtgg
     3901 cgagagccgg actgacatta cggattaaat acatataagc gttgatggcg ataatcgaac
     3961 caaacagcgc cagatagccg accgcaagga agcctgaaag ggaagggagc gccgtcagtt
     4021 tttcacctgc aatcatcgac gcgatcatta acaccacgcc tgccgccagc atctcaatcg
     4081 cacccgccat catccctaca ggtaaggtaa tgcgcgagcc ataaaccgag ccaaacgccc
     4141 agctaatcga gccgattaaa atcagaatcg cgccccacgg attgccgctt aaatttccac
     4201 cgctattgag catgatgatt ccggcaagcc caatggcgat acccacccat tccaatttgc
     4261 gcgttttaat gccaaacagg cggctgaagc acagggtaaa gaggggcacg gttgcaacca
     4321 caacggcggc gatgccggaa ggaacatttt gatgctcggc aaccgtcacc atgccattac
     4381 cgacagccag caataacagg ccaatcagcg cggcattgag cagcggacgt agcgggggga
     4441 gcttgtgtcc gcgcagtagc aaaaatgcca tcaataaaat accggctgcc aggaatcgaa
     4501 cgcctgccat cattaacgga ggccagcttt ccacgccaat ccgaatgaca aaataggttg
     4561 agccccaaat gatatacaac gcaaacagcg cgccaaaaag cggtaacaac tggcggaaac
     4621 gcataatccc tcacggtgga aataaaaagg tggttcatag taaacgtgaa aatcattctg
     4681 ctggcgagag atataattgc atttgattgt taaaaaaatg ttgacctgtg aagcaagtat
     4741 cagagctgcc gtttttgctt catacttaca cccttcaaca ataaaaatga gagggaatgc
     4801 ttttggccgg gagtagttta ctgacgttgc tcgatgatat cgccacacta ctggacgata
     4861 tctccgtgat gggcaaactg gcggcgaaga aaaccgccgg tgtattaggg gatgacttat
     4921 cgctcaatgc gcaacaagtt tcaggcgtgc gggccaaccg ggaacttccc gtggtctggg
     4981 gcgtggcgaa aggatcgctg attaataaag tgattctggt gccgctggcg ctgatcatca
     5041 gtgcgtttat cccgtgggcg attacgcctc tgttgatgat tggtggcgcg tttctctgct
     5101 ttgaaggagt agagaaagtg ctgcatatgc tggaggcgcg taaacataaa gaagatccgg
     5161 cgcagagcca gcagcgtctg gagaagctgg cggcgcagga ttcgctgaag tttgaaaagg
     5221 acaaaataaa aggggcgatt cgtaccgatt ttatattgtc tgccgaaatc gtcgccatca
     5281 cgctggggat tgtggccgaa gcgccgttgc ttaatcaggt gctggtgctt tcaggcatcg
     5341 cgctggtagt gaccgtgggc gtctacggtc tggtaggggt tatcgttaag attgatgacc
     5401 tggggtattg gctggcggaa aaatccagcg cgctgatgca ggcattaggt aaagggttat
     5461 tgatcattgc gccctggctg atgaaagcgt tatcgattgt cggcacgctg gcgatgttcc
     5521 tcgtcggcgg cgggattgtg gtacatggta ttgcgccgct gcatcacgcc attgaacatt
     5581 tcgctgggca gcaaagtgca gtggtggcga tgatattacc gactgtttta aatctgattc
     5641 ttggatttat catcggcggc atcgtggtgc tgggagtgaa agccgtagcg aaaatacgcg
     5701 gtcaggcaca ttaatactcg cacaggctat tatgcaaaat tcgccatctt caactaaggt
     5761 gaacaagttt cactcgaaaa aaggaggcga gtatgagctt tatggttagt gaggaagtta
     5821 cggttaaaga gggcggcccg cggatgattg tcaccggata ctccagcggt atggttgagt
     5881 gtcgttggta tgacggttac ggggtcaagc gggaagcttt tcatgaaacc gagcttgttc
     5941 cgggggaggg gagtcgttct gcggaagaag tttgaaaggg tatcattaac accaggccgg
     6001 atcacttccg gcctggtgtg ccaatgcctg caatgaatga tagtcgcgtc ttatcatgcc
     6061 accattataa aattatcgct gttttgcggt acgctatcgc caggttatgc cagaatcata
     6121 aaaaagcagg ttgggagtcg tcagggtgca gcacgagaca aaaatggaaa accagagctg
     6181 gttgaaaaaa atcgcacgcc gcctggggcc tggtcatgtc gttaatctct gctttatcgt
     6241 ggtattgctt ttttccacct tgctgacctg gcgtgaagtg gtagtgctgg aagatgccta
     6301 tatctccagt cagcgtaatc atctggaaaa cgtagccaac gcgctcgata agcatttgca
     6361 gtataacgtc gacaaactga tctttttgcg taatggcatg cgcgaagctc tcgtagcgcc
     6421 actggatttc acttcactgc gtaatgctgt aaccgagttc gaacagcatc gcgacgagca
     6481 cgcctggcaa attgaactca accgacgacg caccctgtca gtcaatggcg tatcggatgc
     6541 attagtcagc gaggggaatc tcctgtctcg cgaaaatgaa agcctcgaca atgaaattac
     6601 cgctgcactg gaagttggtt acttgctgcg actggcgcac aacacctcgt cgatggttga
     6661 acaggcgatg tatgtctcgc gtgccggatt ttacgtttcg acgcagccga ccttgtttac
     6721 gcgcaatgta ccaacgcgtt attacggcta tgtcacccaa ccctggttta tcggccattc
     6781 gcaatgagaa aatcgtcacc gcgcggtacg ctggtttact tcgcaaccgg aacacgccag
     6841 caatactgaa ccgcaggtta ccgtcagtgt tccggtagac agtaataact actggtatgg
     6901 cgtgctgggg atgagtattc ccgtgcgtac catgcagcaa tttttaagaa acgccatcga
     6961 taaaaacctc gatggtgagt atcagctcta tgacagtaag ctgagatttt tgacctcttc
     7021 caatcctgac catccaacag ggaatatttt tgatcctcgt gaactggcct tgctggcgca
     7081 ggcgatggaa catgacacgc ggggcggcat tcgtatggac agtcgctatg ttagctggga
     7141 acgtctggac catttcgacg gtgtgctggt gcgcgtccat acgctaagcg aaggcgtgcg
     7201 cggcgatttc ggcagtatca gcattgcatt aaccctgctg tgggcgctct ttaccaccat
     7261 gttactcatc tcctggtatg tgattcgccg gatggttagc aacatgtatg ttctgcaaag
     7321 ctcgttgcag tggcaggcgt ggcacgacac cttaacccgt ttatataatc gtggcgcact
     7381 gtttgaaaaa gctcgtccgc tcgcgaaatt gtgtcagacg caccaacatc ctttttctgt
     7441 cattcaggtc gaccttgacc attttaaagc gattaatgac cgctttagtc atcaggcggg
     7501 cgaccgtgtt ctttctcatg ctgccggatt aattagcagt tccttgcgtg cgcaggacgt
     7561 tgccgggcgg gtcggtggtg aggagttttg tgtgattctg ccaggcgcga gtctgacgga
     7621 ggctgcggaa gtcgcagaac gtattcgcct gaagttaaat gaaaaagaga tgttgattgc
     7681 taagagtacg acgatacgca tcagtgcctc gttgggggta agtagcagcg aggaagccgg
     7741 tgattatgat tttgaacaac tccagtcact ggctgaccgt cggctttatc tcgctaaaca
     7801 ggccgggcgt aatcgggtat gcgcgagcga taacgcttaa cgagcggaga aaaaatggtc
     7861 cagcccttca cgccatcctt ccggtccttc acgctgcgtt cgccagacgc gggccggatc
     7921 ctcatcatgc agatgcaccc cttcacggtt tagccctttc acaatcaccg cgtaatccat
     7981 tacctccagt aagggcgcat cgtttggccc atcgcccagg ccaagtgtgg ttgggcgttt
     8041 gcctgataat tgttgatagg tcgcgataat ccagttggcg gcctgatctt ttccggcaga
     8101 ggcatccagg acgtgccaga agcgcgcacc ctgcataaac tgtaagccca gttcgttcag
     8161 acgagcggta aattgtgcca tacgctcgtc actgtcgcgc cagattagcg ttaccgacgc
     8221 ctcatgcagc tgcgtcagcg ccgcctggct acggcttaat cccgtccatt cggcgatggt
     8281 tgcatcgtcg acatcatcga aagtcgtgaa tttaaaatgt tctttctcgc gtagcgtatt
     8341 taaaacctgg ctgatttcgc catggctaat acctgagatg atgcgtggaa aaccgtctat
     8401 atcctgccat tgttcagcaa gctggatcac tgcgccattt tcggcaatca gtggtacccc
     8461 ttgtaacccc aacgtttttt gcaagtacag catttccgct gaagttttac tgctacagag
     8521 aatgacggga acattcgctt cgcgtaaacg gctgagccag ggggcagccg gttgccagtc
     8581 ataactatga ctgtccagca gggtgccatc aagatcgcta aaaaccagta gtggttgttg
     8641 aattgaaaac atggtcgcgc agttctcccc ttaccaggat gattctcaaa acagcacaaa
     8701 taatcaaaga tgattttttc gggagcgatc ccgttgacgc cagagccacc aaggggtgac
     8761 gcacgtcaca tttctattca taagtagcgt taatcattca tatcgcgaat attttcttgt
     8821 cagcgaaaaa aattgcggat aaggtgatga acacatcaga tttcctggtg taacgaattt
     8881 tttaagtgct tcttgcttaa gcaagtttca tcccgacccc ctcagggtcg ggattttttt
     8941 attgtgcatt caacgattca cttcagagac gctgaagtca tgaatatcca gttcgaaggc
     9001 atccgccaat tcacgccatg tatgatattc acgtccatcg acgctaacgt gttcagaatc
     9061 gttctggctg cgatgaactt atttcattga tcgccgccag cagggcatcg acatcgacgc
     9121 tgacctcacg ttttgcggta tcgctgtact cttttgcggt tttcatcatc tttcctcgca
     9181 accgttactc tgttgttgcc gccagtcgtt aagtccgcct ggcatctctt aagtatagac
     9241 cgtcgagaaa atcagcactc gcgcgcgcct ggcgctgcca gaaaaacgaa attgttctac
     9301 actggcacaa agccacagga ggaaaacgat gaaggtgaat gatcgggtaa cagtcaaaac
     9361 ggatggcggt ccgcgtcgtc ctggcgtggt actagcagtt gaggagttta gtgaaggcac
     9421 aatgtacctg gtttcgctgg aagactaccc gctcggcatc tggttcttta atgaagcagg
     9481 gcatcaggac ggtatctttg tggagaaagc agagtaatct tattcagcct gactggtggg
     9541 aaaccaccag tcagaatgtg ttagcgcatg ttgacaaaaa taccattagt cacattatcc
     9601 gtcagtcgga cgacatggta gataacctgt ttattatgcg ttttgatctt atgtttaata
     9661 ttacctttat gcgatgaaac ggtcttggct ttgatattca tttggtcaga gatttgaatg
     9721 gttccctgac ctgccatcca cattcgcaac atactcgatt cggttcggct caatgataac
     9781 gtcggcatat ttaaaaaaga ggttatcgtt gtctcttttt tcagaatatc gccaaggata
     9841 tcgtcgagag attccggttt aatcgattta gaacttatca ataaattttt tctgaccaat
     9901 agatattcat caaaatgaac attggcaatt gccataaaaa cgataaataa cgtattggga
     9961 tgttgattaa tgatgtgctt gatgtgctga ctgttagaag catcatggat gaaacagtcc
    10021 tcattaataa acaccactga agggcgctgt gaatcacaag ctatggcaag gtcatcaacg
    10081 gtttcaatgt cgttgatttc tcttttttta acccctctac tcaacagata cccggttaaa
    10141 cctagtcggg tgtaactaca taaatccata ataatcgttg acatggcata ccctcactca
    10201 atgcgtaacg ataattcccc ttacctgaat atttcatcat gactaaacgg aacaacatgg
    10261 gtcacctaat gcgccactct cgcgattttt caggcggact tactatcccg taaagtgttg
    10321 tataatttgc ctggaattgt cttaaagtaa atgttgcgat atgtgagtga gcttaaaaca
    10381 aatatttcgc cgcaggagta tcctggaaga tgttcgtagg tattattttt cggataatcc
    10441 ttaggataac atgataaacg ttacggaatt atattaatgg caattcacta ataatatcag
    10501 ccagcaaatt aaacatctca ctgaataaat gttcgcaaaa aggtgcaatt aacggcatta
    10561 atgccgccat taaagagata ccgacagtca gagttaatgg aaatccaata acaaaaatgg
    10621 ataattgcgg ggccatacga ttcagtaaac ctaatgccag attcagggtc agcagcagag
    10681 taatgagcgg taacgccagc atcagcccgt taaggaaaat caaactcccc gctttggtga
    10741 gtgccagaaa cgcattgctg ttcaacggtt cgccaccaat tggcagggtg tgaaaggtat
    10801 cgaccagcag tgaaatcaac cataaatgac cgttaaatgt caggaacagc agtaacgcca
    10861 gcatatccat gatacgcgct aaaacgggca tattgagatg gctggccgga tcgacaaacg
    10921 tcgcaaatga cagccccatt tgcagaccga taatttcgcc agcggttcgc acagcggcaa
    10981 aggcaaattg catggtaaaa ccaagcgcaa tgccgatcag gatctgctgc acggccagcc
    11041 acagagcaaa gaacgaaaaa acaggaacat cgttggcagg taatgatggg gcaatgacga
    11101 acgtgatcat cattgccagc cccagtttta cccgtttcgg cacgctgcgt tcgctcagaa
    11161 ttggtgcggt agaaatgagc gccagcacgc gcagtaacgg ccagaagtac aggcttaacc
    11221 aggaaagcca ttgatcgctt gtcacctgca tcattgtacg gcctacccga tgatatacgg
    11281 caggttagtg aacaaggtgc ggacgtaatc cagcaacaga ttgagcatcc acggtccggc
    11341 aataataatg gcaataaata cggcgatgat ttttggaata aacgacagcg tcatttcgtt
    11401 gatctgcgtg gcggcctgca aaatactgat gataaggccc gtgaccagcg ccaccagcaa
    11461 cagcggggca gccagtgcca gcgcgacttt catcgcttca gtccccatca tcatgaccga
    11521 ttcaggtgtc attttgcctc tctagctgta aaagctctgc gccagcgaac cgaccagcaa
    11581 ttgccagcca tccaccagta caaacagcat cagtttaaag ggcagagcaa tggtggcggg
    11641 gggaccatca tcatccccaa tgccatcaac acgctggcaa tcaccaggtc gataatcaaa
    11701 aaaggaatga aaatcgtgaa gcctatctga aatgcggttt tcaactcgct ggtcacgtag
    11761 gccgggcgca aaatgcgcat cggcacggct tcaggtccct gcaacgggcc ggtattcgct
    11821 agtctggcaa acaaccctaa atctgcctca cgggtctgac gcagcataaa ctcacgtagc
    11881 ggctgcgccc ctttttccag cgcctcctgc attgatattt tctcttcgct gaatggctgg
    11941 tacgcatcga cataaatttt gtcgatcacc ggtgacataa taaaaaaggt caaaaatagt
    12001 gccagcccca gcaatacctg gttaggtggc gcggagggcg ttcccagcgc gttacgcaat
    12061 aaaccaaaaa caatgatgat gcgggtgaaa ctggtcatca tcagtaaaat tgccggaata
    12121 aacgtcaacg aggtgatgaa caccagcgtc tgcaccggga gcgaccagct ttgtccaccg
    12181 ccaggcagcg gctggctggt gatacccggc agttgcgcga aggcgagggg cgtaaccagc
    12241 cagagaagga caggtgcgac agacaacaaa cgacgcatca ggatctccca ctacgcttaa
    12301 gcaaattttt catgaccgac tgaaaatcgg tctgcggtat ctcttctgtt ggtgctgaag
    12361 ggggaagttt atgcagcaga ttgatttgcc ctgcggtaac gccgagcacc agccgtgcat
    12421 cttccacatc gaccaccaca acccgttcac gcgcgcccag cgaagcactg gcgctaattt
    12481 tcagaccgtt aacgccagtg cgtttcgggg caaatcccaa ccgttttacc tgccaggcag
    12541 cagcgaggat cagggcaata atggcgatga gtgcgccgct gacctgcagc agtggcgcag
    12601 cagaaaccgg cgtggaagat tgcacagtag cgtggttatt catcactaac ggctcaggcg
    12661 atgcattcgc tcagacggag taatgatatc ggtgatccgc acgccatatt tatcggcaac
    12721 gaccaccact tcgccctggg cgattaaata accgttgatc agaatatcca gtggttcgcc
    12781 cgccagaccg tccagcgcca cgacggaccc ttgcgtcaga cgcaacagct ctttgatggt
    12841 catccgcgta cggcccagct cgacggtcag cttgaccgga atatccatta tcaggtcgat
    12901 atcctgcaac gttccgctga catcaccacc gccaaattgc tggaacaccg cgtcggcagc
    12961 gcttttgctg ctggttgatt tttgttcgct caacgcttca gcccacagat cgtccattgc
    13021 gccgttgttg tcatcggccg gattattcat gtcactcatt tgggctgttc ctcgttcaga
    13081 gaatttaaaa tcgggttaat caaatgttct atccgtaacg catactgacc gttgagagtg
    13141 ccgtactgac tggtcagcac cgggacgccg tcaacatggg cgatgatgcg atcaggtttt
    13201 tctatcggca ggacgtcgcc gggtttcagt tttaaaatct gcgacaggcg tagcgagata
    13261 tcggcaaagt tggcgaccag ctccagctgt gaatgctgca cctggcgcac caggttatcg
    13321 cgccagttct gatcttcatt acgcgagttt tccagcggcg ggttaaccaa caattcccgt
    13381 agcggctcga tcatgctgaa tggcagacag atattaaatt cgccggtcag gttgccaatc
    13441 tccacatgga acggcgtgtt aaccacaatg tcgttcggcg aggtggtgat attggtaaat
    13501 ttcacctgca tttccgaacg cacgtactca acttccagcg gattaatcgc cttccaggcg
    13561 tcgctatagc cttcaagcgc cagctttaac atgcggttga tgacgcgctg ttcggtatgg
    13621 gtaaactcgc gaccttccac tttggtcggg aagcgtccat cgccgccaaa caggttatcc
    13681 acggcgataa acaccagact cggtgaaaac accaccagcc ctgtgccgcg cagcggtttc
    13741 agatggataa ggttcaggtt ggtcggcacc ggcaggttgc gggcaaattc atggtacggc
    13801 tgaatgcgga tggccccgac ggttatatcc gggctacgac gcagcaggtt gaacagcccc
    13861 atacgaaaat ggcgggcaaa gcgctcatta atgatttcca gcgcctgcaa acgttcgcgc
    13921 acaacccgtc gttgggtatt cggatcgtac ggacgaatgt cactttcgcc gctaacactg
    13981 gctgtcggtt cgtctttgac ttcgctgtca ccattcaaca gcgcatcaat ttcagcttga
    14041 gaaagaatac tatcgcccat gtcgttatcg cagaataaaa gcggtataca gcacgtcggt
    14101 gacatcctgt ttcggttgcc cggcaacaag cggggtggaa agcgtggttt taatctcggc
    14161 aatcaggttt ttcttgcctt cttctgtcgc cagtacggca gcatcctgac gcgaaaacag
    14221 taacagcaaa cggctacgga cttccggcaa atactcactc agccgcgagc gggtagcttc
    14281 atctttcagg cgcagggtta tgccgatata aagcacgcga tccgcatcgc ccaaattgac
    14341 cgtgaaggta ttcagcgcgt agaagaccgg ggagggtacg acgcgttgct gcgctttgtc
    14401 gtcagcggca acctgatgcg aatgccagta gctgtaacct gcgctggcac aggccgcgag
    14461 ggtaatgaat accagaatcg ggatccaaag cgatcgcttg cttttcttgc ttatcgcgta
    14521 atcagtcatg tgttgcggtc ttcctgtgtc gctactgctt atcggctaat tatcctgcgt
    14581 tttgagcgca acaaagcggg gaaaagacgc ggattacggt gctacctctg acgttaggcg
    14641 aaaatatcaa cgccgctgtt gcctgttaca cgcccttgta aagagacggg aaccggaagc
    14701 gtatcgtcgt cttcccccgc cagaggttca tggtttactg tgcgttggct ttgctgttgt
    14761 tgggaagcgg cctgctgctg accactaaag ctttcgccac tgatgttgct ttgccctaac
    14821 tgaatgccac tttcggccag ctgcgtacgc agtaccggaa gcgctgcttc cagcgcggcg
    14881 cgtacatgct gatgcggtga aaccatctgg atttgcgcct ggttatcatc cactttgagg
    14941 gagatttgca cttcacctaa atcctgcggg tgcagacgca actctgcact ttgttgcccc
    15001 tggcgggtga acagcgaaat atgctggctt aatgattgtt gccattcgtg agaacccagc
    15061 ggtgcactca aaacaggcgc ggcgacggtg ggcagtggct gtgtctggtg tggagtaatt
    15121 agcgggctgg cggcagcggt caccggtgac ggtgtgctga tgacttccgc tttactctgg
    15181 gcttctgcta ccagcggtgt taatggctga gctggcgtgc cgggggcatc atcaggctgt
    15241 gctgttgtga gttgcgcaga agtcagtttt gtgaagagcg ttggtttctc tgccggtaac
    15301 acggttgacg gcacatcagt cactttgggc gtattgtcaa aacccggcaa catcgcaaaa
    15361 agggcgctca ggcttgcggt gacgtcttca ttcagatcat ccgctttttc gtcttttgtc
    15421 gtattttggt cagccaccgc agccaacgtc atcgtctgag cggtggttaa cggtgttgat
    15481 gtggattgtt cgtcgttgat gacaggcagt gtttcatcca cggggatcag taaatccgct
    15541 tgttgcgcgt cggcaagaat atcgctgatc agcggctcgc cttttgtcgt gggcttatct
    15601 gttgccacca gcaactgggg ggccgctttg tcggtagttg tctcgcctgc taatgcttcg
    15661 ctcaacaacg cgagaaaatc ttgtgcagca tcgctggctt tgccgccagg caatgtggtg
    15721 gtgtcaacgt cggcggtaat taagggcgct aagcgaatca ttcaggtttc ctcatggcgg
    15781 cgcgctgggc gaactcatcc atctttttct gatcgaggcg gttttctgca agcagtgccg
    15841 ccgtggatag ccgttcctgc agtgtctgcc aggcctgcaa acgttgtttc ttttctcgcc
    15901 agctgttcag ggcaatgtca actttctgcg tccactgatt aagttgctgg cgatgctgag
    15961 taatggcttt ttccagcgtc tggataaact gctgatagtt gatccagcgg ttgctggtca
    16021 tcccggcact catatcgctg ttgaggttat tgcgatattc attctgataa tcgatcagca
    16081 ttttgagctg ttcttccgcc tgctgacacc cgcgacgcat ttcacccagc aggcgcgcgg
    16141 catcctctac ctctttttct gccagatctt tcagggtcgc cagcgcgcca tgttctgcca
    16201 tctgccgtta tctcctgggt tatgacactg tcgggaaaat acgctccagc ccctggagag
    16261 acgcttccca gtccgcgcgt tcaaaaatgc cttgttgcaa atagccctcc agctgcggcc
    16321 acagggcgat ggctttgtcg agcatcggat cgctgccttt ggcatacgcg ccgacgctaa
    16381 ccagatcgcg gttacgctga aaactcgaca acagctgttt gaaggtgcgc actcgcgcgt
    16441 aatgttgctc actgatcaac gccgtcattg cgcggctgat cgacgcttca atatcgatag
    16501 ccggatagtg cccggcttcc gccagtcggc gagacagcac aatgtgaccg tcgaggattg
    16561 cccgcgcaga gtcggcaatg gggtcctgct ggtcatcgcc ttcagtgagc acggtataaa
    16621 acgcggtaat cgatccgccg ccgctaatgc catttccggc acgctcgacc agtgccggta
    16681 atttggcaaa caccgacggg ggataacctt tagtggcggg tggttcgcca atcgccagcg
    16741 caatttcacg ctgggccatc gcgtagcggg tgagggagtc cataatcagc aacacatgct
    16801 gaccacgatc gcgaaaatct tcggcaatgc gcgtggcata ggcggcaccc tgcattcgca
    16861 ggagaggaga aacatccgcc ggagcggcaa tcaccacgga gcgtgcacgc ccttcggcac
    16921 cgaggatatt ctcaataaaa tcttttactt cgcgtccgcg ttcaccaatc aaacccacga
    16981 caatgacatc ggcgcgggtg taacgtgcca tcatccccag cagcacactt ttaccaacgc
    17041 cggacccggc aaacagcccc atacgctgcc cacgcccaac ggtaagcagg gcattgattg
    17101 ggcgcacgcc ggtgtccagc acatgttcaa tcggtgtacg ttgcaacggg ttaaatggcg
    17161 gggtaatcag cgcaccggtt tccgtcgtat cgggggaggc catcgagcgg tttaccgctg
    17221 ccgtccagaa cacgacctaa taacgccgga ccgagcggta actgtttgcc gctttgcagc
    17281 ccttctgccg aaatgttttt ggcataaaca cgcgcgccgg gcaggacacc ttcgacttcc
    17341 tccagcggca ttaaaaacag ccgttgaccg ttaaagccaa cgacttcact ttctacttcg
    17401 tgcgtttcag tgccgttctg gcgctcaatc acacaggttg cgccgagcgg caattgtaat
    17461 ccggtggctt ccagcaccag cccggtagcg cgggttaatc gcccgtagcg acgtaccgca
    17521 ggcaactgcg ccattttggc ttcaaagtta tccagcgtgg ttagccagcg agtcaggcgc
    17581 gtggtcatta caccactcct ggtgctgcca gacggcagag ttcttgccag cgagtggcga
    17641 cactggcgtc gagatcgcct tcatcggcgg agactttaca gccgccagga tggagggtgg
    17701 gatcgccccg caagcgccag ccatgcaaac ttaaggtagc gccgagcata tcatccacac
    17761 gttgcagatc atccgggtgc acgcgcagct gtggtttacc gctgaataac ggttcttgct
    17821 gcaacaactg ttggatctgt ttgatgagtg ccgagttatc cactgttggc gtctgaccga
    17881 tgacctgacg tgctgcctcc agcgccatct gcatcaggcg cgaagc
//
LOCUS       DAJVUJ010000081        17793 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.081, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000081 DAJVUJ010000000
VERSION     DAJVUJ010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17793
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.081"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..387
                     /locus_tag="OQQ48_002442"
     CDS             <1..387
                     /locus_tag="OQQ48_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HCS1953180.1"
                     /translation="FLAEVRQHTWSSGTNGVVAATFSLRLKGKPVSFVVPLAFVKNLD
                     KTLTVNTGALLTMSVSANGGMPPYKYAWKKDGQPVDGQTTDTFSKPGAQSADAGKYTC
                     VVTDSAEKAQSVTSVECTVTVSAAAG"
     gene            402..776
                     /locus_tag="OQQ48_002443"
     CDS             402..776
                     /locus_tag="OQQ48_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HCS1953181.1"
                     /translation="MKKDLKTLALARLSGFRHKTVKVPEWGNVSVVLREPSAEAWYLW
                     QEVLNGDGEDDDTLSVVAKTRRNLEADVTLFCDVLCDTDLQRVFTPDDREQVLAVYGP
                     VHARLLRQALELIADAESARKK"
     gene            872..1081
                     /locus_tag="OQQ48_002444"
     CDS             872..1081
                     /locus_tag="OQQ48_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HCS1953182.1"
                     /translation="MWMEFDRISPLGDERGDIRNAQIVKAVFGAQGMNVALKDVMLCW
                     GEDEDKPEVDPFAALEDALSFAAQS"
     gene            1129..1389
                     /locus_tag="OQQ48_002445"
     CDS             1129..1389
                     /locus_tag="OQQ48_002445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1953183.1"
                     /translation="MATLRELIIKIVSFRGTHIDAFFRRASSAAHTNARGLITVDLFC
                     CTEAIARQVPEAGSEVLSSVIASGREVLTRGLIAGDDVLTRE"
     gene            complement(1524..3125)
                     /locus_tag="OQQ48_002446"
     CDS             complement(1524..3125)
                     /locus_tag="OQQ48_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc49 family transposase"
                     /protein_id="HCS1953184.1"
                     /translation="MNIRIWSGILPCMDISALNTTNDIEKLRAMALAMVQEVMSENAE
                     KERELLEKSRRIQLLEEMLKLVRQQRFGKKCETLAGMQRSLFEEDVDADIAALTAHLD
                     KLLPQSPEEDEKASRSRPIRKPLPVHLPRVEKMIQPDTDHCPECDEPLHYIRDAVSEK
                     LEYIPAHFVVNRYVRPQYSCPCCQKVFSGEMPAHILPKSAVEPSVIAQVIINKYGDHL
                     PLYRQQQVFARSDVGLPVSSMADMVGAAGAALSPLAALLHRELINRPVVHADETTLKI
                     LNTKKGGKSCSGYLWAYVSGERTGPSVVCFDCRTGRSHEYPENWLQGWGGTLVVDGHK
                     AYRTLANKVPEITLAGCWAHARRGFADLYKISKDPRAAIAVKKIAGLYRLEKKISSRP
                     VEKIRQWRQRYARPILEELWSWLEEQEPQCSPGKALHKAIAYALSHRVELSRFLEDGA
                     VPLDNNVCERAIKNVVLGRKSWLFAGSQMAGERAAQIMSLLETAKRNGLEPHAWLTDV
                     LMRLPEWPEERLAELLPLEGFTFSG"
     gene            complement(3132..3524)
                     /gene="tnpB"
                     /locus_tag="OQQ48_002447"
     CDS             complement(3132..3524)
                     /gene="tnpB"
                     /locus_tag="OQQ48_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCS1953185.1"
                     /translation="MLSPDNVFIAIKPVDMRRGIDSLTQYIQDELRSTWHEGAAFVFV
                     NKVRSRIKVLRWDKHGVWLCTRRLHKGSFRWPRANDAAWHLTPDEFNWLIAGVDWQQV
                     KGHDLTKWVWQNEPELRPENTKNTLLTQ"
     gene            complement(3511..3840)
                     /locus_tag="OQQ48_002448"
     CDS             complement(3511..3840)
                     /locus_tag="OQQ48_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HCS1953186.1"
                     /translation="MSKPRWTIEQKRQHVAAWRASGLTRQQYCELNDIPFTSLREWPQ
                     DVAKAERRANEPTVLPVHIAPPLHADVPQPVTNEPVTLFLPGGVRMCCQPSQLTDVFR
                     ALKYAQS"
     gene            3885..7145
                     /locus_tag="OQQ48_002449"
                     /pseudo
     CDS             3885..7145
                     /locus_tag="OQQ48_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304285.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
     gene            7138..7479
                     /locus_tag="OQQ48_002450"
     CDS             7138..7479
                     /locus_tag="OQQ48_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1953187.1"
                     /translation="MAEIKTLHLVPREGMQVSEKPSVARVRFGDGYEQRRPTGLNARL
                     KTFQAVFRVTDEATRRWLEEFLSWHGGYRAFLWRPPKHNRTVRVVCREWSVTDNARYS
                     DFSCTIEQVVN"
     gene            7479..8177
                     /locus_tag="OQQ48_002451"
     CDS             7479..8177
                     /locus_tag="OQQ48_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCS1953188.1"
                     /translation="MQDIHEESLSESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGRKYEAYPIDGSGFEMNGRGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELSDRWVVEQMSQLTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWTYRSDECGYTGGAVADEFDKPTTDIRKDRCSKCMRGCELRRNV
                     GNFGGFLSINKLSQ"
     gene            8188..8931
                     /locus_tag="OQQ48_002452"
     CDS             8188..8931
                     /locus_tag="OQQ48_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1953189.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVISTPEGERYIPCVNISAEPE
                     AYFRIAPEDWLRAEMQGEIVALVHSHPGELPWLSEADRRLQIKSALPWWLVCRGEIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIDMPDFHREDDWWRNGQNLYLDNMAVT
                     GFYRVPLSSAPPGDILLCCFGASVPNHATIYCGNGELLHHLPEQLSKRERYSEKWQRR
                     THSVWRHRHWHASAFTGICNDLAAASACM"
     gene            8829..9509
                     /locus_tag="OQQ48_002453"
     CDS             8829..9509
                     /locus_tag="OQQ48_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCS1953190.1"
                     /translation="MATTNAFCLASPPLARICLHGDLQRFGRRLSLYVNTAAEAIRAL
                     SMQMPGFRRQMNEGWYQIRIAGDDTAPEAVYARLHEQLGEGTVIHIVPRLAGAGKGGL
                     QIVLGAAAIVGSFFTAGASMALWGSALAAGGFSATTMLFSLGASMILGGVAQMLAPKA
                     KTPDYRATDNGRQNTYFSSLDNMIAQGNPMPVPYGEMLVGSRRISQDISTRDEGGGGK
                     VVVIGRQG"
     gene            9750..13223
                     /locus_tag="OQQ48_002454"
     CDS             9750..13223
                     /locus_tag="OQQ48_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCS1953191.1"
                     /translation="MGKGGGRAHTPREAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPESFESSGSETVLGVEVTRAKPVTR
                     TITSANIDRLRVTFGVQSLVETTSKGDRNPSSVRLLIQLQRNGNWVTEKDVTINGKTT
                     SQFLASVILDNLPPRPFNIRMVRETADSTTDQLQNRTLWSSYTEIIDVKQCYPNTAIV
                     GMQVDAEQFGGQQMTVNYHIRGRIIQVPSNYDPEKRTYSGIWDGSLKPAYSNNPAWCL
                     WDMLTHPRYGMGKCLGAADVDKWALYAIGQYCDQTVPDGFGGTEPRMTFNAYLAQQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDVVWPYTNSDVVVDDNGVGFRYSFSAL
                     KDRHTAVEVNYTDPQNGWQTSTELVEDPEAILRYGRNLLKMDAFGCTCRGQAHRAGLW
                     VIKTGLLETQTVDFTLGSQGLRHTPGDIIEICDNDYAGTMTGGRVLSIDAATRTLTLD
                     REVTLPETGTSAVNLINGSGKPVSVDITAHPAPDRIQVSTLPDGVETYGVWGLSLPSL
                     RRRLFRCVSVRENTDGTFAITAVQHVPEKEAIVDNGARFEPQSGSLNSVIPPAVQHLT
                     VEVSAADGQYLAQAKWDTPRVVKGVRFSLRLTSGSGEDSRLVTTAITADTEHRFSGLP
                     LGEYTLTVRAINSYGQQGEPATTTFRINAPAKPATIELTPGYFQITAVPRLAVYDPTV
                     QFEFWFSEAKIADTSQVETSARYLGTGSQWSVSGPHIKPGKDFWFYVRSVNLVGKSAF
                     VEASGRASNDAEGYLGLFREKIGKLHLAQGLWELIDNSQLADEMAEMKTTITETRNEI
                     TQTVSKTLEDQSATIQQIQRVQKDTNDDLAALYMLKVQKTKNGIPYVAGIGAGIEDTD
                     GQPLSNILLLADRIAMINPEDGNTTPLFVAQGNQLFMNDVFLKRLFAVSITSSGNPPT
                     FSLTPEGRLTARNADISGNVNANSGTLNNVTINENCRVLGKLSANQIEGDLVKTVGKA
                     FPRDSRAPERWPSGTVTVRIYDDQPFDRQIVIPAVAFSGAKHEREHTDIYSSCRLIVR
                     KNGAEIYNRTALDNTLIYSCVIDMPAGHGHMTLEFSVSAWLVNNWYPTASISDLLVVV
                     MKKATAGISIS"
     gene            13291..13890
                     /locus_tag="OQQ48_002455"
     CDS             13291..13890
                     /locus_tag="OQQ48_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCS1953192.1"
                     /translation="MRKVCAAILSAAICLAVSGAPAWASEHQSTLSAGYLHARTNAPG
                     SDNLNGINVKYRYEFTDALGLITSFSYANAEDEKKTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDIAYEGSGSGDWRTDGFIVGVGYKF"
     gene            13955..15362
                     /locus_tag="OQQ48_002456"
                     /pseudo
     CDS             13955..15362
                     /locus_tag="OQQ48_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422246.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
     gene            15448..15960
                     /locus_tag="OQQ48_002457"
     CDS             15448..15960
                     /locus_tag="OQQ48_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1953193.1"
                     /translation="MGVTSGWVGSSAKSETGEQWMGAAGTKLGLSKPFMMSQMVGRTM
                     GCKIATEYYKWKSSDKVDNWGAVGADWPLEEKSKGTITNAASCGSGRLVGAVVTLSHF
                     LTNSTPTAAVYLAGGKAGNITVNVGGATQTMIYQGVVSGFQYYWSGSVSSAFVEAMKK
                     TGVPQDLKIS"
     gene            16036..16248
                     /locus_tag="OQQ48_002458"
     CDS             16036..16248
                     /locus_tag="OQQ48_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HCS1953194.1"
                     /translation="MPEKFFRTDADNNDVPMTAASWMALSEATEQAMFAKGVEINTRQ
                     LQMKAEVEALTDLKAIRSYVVGWPAG"
     gene            complement(16390..16714)
                     /locus_tag="OQQ48_002459"
                     /pseudo
     CDS             complement(16390..16714)
                     /locus_tag="OQQ48_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            complement(16850..17098)
                     /locus_tag="OQQ48_002460"
     CDS             complement(16850..17098)
                     /locus_tag="OQQ48_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCS1953195.1"
                     /translation="MRIEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(17453..>17793)
                     /locus_tag="OQQ48_002461"
     CDS             complement(17453..>17793)
                     /locus_tag="OQQ48_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCS1953196.1"
                     /translation="KVLPENWWQHPAALGATDSDIEIIKRQWGAFYGTDLELQLRRRG
                     IDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAASAEQHNNSINHIYPRIARV
                     RSVEEILHAL"
BASE COUNT         4193 a         4290 c         5435 g         3875 t
ORIGIN      
        1 ttcctggcgg aagtgcgtca gcacacctgg tcatccggta ccaacggtgt ggtggctgca
       61 acgttctcac tgcgtctgaa aggcaaaccg gtgtcctttg tggtaccgct ggcgtttgtg
      121 aaaaatctgg ataagacact taccgtgaat accggtgcgc tgctgacaat gtcagtcagt
      181 gccaacgggg gaatgccgcc gtataaatac gcctggaaga aggatggtca gccggttgac
      241 gggcagacga cagacacctt cagtaagcca ggtgcgcagt ccgctgatgc ggggaaatat
      301 acctgcgtgg tgaccgattc ggcagagaaa gcacagagtg tgacgtctgt tgaatgcacc
      361 gtgacagtga gcgcagctgc cggataaggg gatgggtcat catgaaaaag gatctgaaaa
      421 cgctggcgct ggccagactg tcagggtttc gtcataaaac ggtgaaggtg ccggaatggg
      481 ggaatgtcag cgtggtgctg cgggagcctt cggcagaggc ctggtatctg tggcaggaag
      541 tgctcaatgg tgatggagag gatgacgata ccctgtcggt ggtggcgaaa acccgccgta
      601 acttggaagc ggatgtgacg ctgttctgcg atgtcctgtg tgatacggac ctgcagcggg
      661 tgttcactcc ggacgaccgt gagcaggtgc tggccgtcta tggtccggta catgcccggt
      721 tgctgcgtca ggcactggaa ctgatcgctg atgcagagtc ggccagaaaa aagtagcccg
      781 cccggaaatt cgctttctga tgcgacttgc gctccgtctg gggcgcacct tatccgaact
      841 gcgccacagc ctgagtgcga gcgaggcgat gatgtggatg gagttcgaca ggatatcccc
      901 gctgggtgat gagcgcgggg atatccgtaa tgcacagatc gtgaaagcgg ttttcggggc
      961 acaggggatg aatgttgcac tgaaggacgt catgctctgc tggggcgagg atgaggataa
     1021 gccggaggtg gatccgtttg cggcgctgga agacgcgctg agctttgcag cacagtcatg
     1081 aatgatgaga accgctgagg cggttttttt acgcccggag aaaggtgaat ggcgacgtta
     1141 cgtgaactga ttatcaaaat tgtaagcttc cggggaactc atattgacgc gtttttcagg
     1201 cgcgcgtcat cggcggcgca tacgaacgct cgtggtttga taacggttga tttattttgt
     1261 tgcacagagg cgatcgccag gcaggtaccg gaggcgggct ctgaagtact gagtagcgtg
     1321 atcgcgtcag gccgcgaagt actgactcgg ggattaattg caggtgatga tgtactgacc
     1381 cgggaatgac tccgggctga ttcaggttct tattcgctgt cagcccgcgt ggttgctggt
     1441 ctggctcatt tcctgccgtc aggagttcaa ctcccgactg caggttatgg cccggtgcac
     1501 gaacacctga cggcaggtat cactcacccg gagaaggtaa atccctcaag aggcagcaac
     1561 tctgccagtc gctcctccgg ccactccggc agacgcatca ggacgtctgt caaccaggca
     1621 tgcggctcca gaccgttgcg tttcgcggtt tccagcaagc tcattatttg cgcggcgcgt
     1681 tctcccgcca tctgcgaacc ggcgaacagc cacgattttc tgcccagaac cacgtttttg
     1741 atggcccgtt cacacacatt attatccagc ggcaccgcac catcttccag gaagcggctc
     1801 agttccacgc gatgagacag cgcataggca atagctttgt gtaatgcctt tcccggagaa
     1861 cattgcggtt cctgctcttc aagccatgac cacagttctt ccagtatcgg acgggcataa
     1921 cgctgtcgcc actggcggat tttttccacg gggcggctac tgatcttctt ctcaagacgg
     1981 tacaaccccg cgattttctt cacggctatg gcagcccgtg gatctttact gattttatac
     2041 aggtcggcga agcccctgcg ggcatgggcc cagcatccgg ccagcgtgat ctccggcact
     2101 ttgtttgcca gagtccggta agctttatgt ccgtcgacaa ccagcgtccc gccccagccc
     2161 tgaagccagt tttcaggata ctcatggcta cgtccggtcc ggcagtcgaa gcacacaact
     2221 gacggtcccg tcctttctcc actgacgtat gcccacagat aaccggagca ggatttaccg
     2281 cctttcttcg tgttcaggat cttcagggta gtctcatctg catgcaccac cggacggttt
     2341 atcaactcgc gatggagtaa cgccgccagg ggagataatg cggcacccgc cgcgccaacc
     2401 atgtcagcca tcgaactgac gggcagcccg acatctgaac gggcaaagac ctgttgctgg
     2461 cgatacagag gcaggtggtc accgtattta ttgatgatca cctgtgcgat gactgatggc
     2521 tcaacggcac ttttcgggag gatatgtgcc ggcatttcac cgctgaacac cttctggcaa
     2581 cagggacaac tgtattgcgg acggacataa cggttcacca caaagtgagc gggaatatac
     2641 tccagctttt cactcaccgc atcgcggata tagtgcagcg gctcgtcaca ttcagggcaa
     2701 tggtcagtgt ccggctggat cattttttcc acccgtggaa gatgaaccgg taagggtttg
     2761 cgtatcgggc gtgaacggga cgctttttca tcttcttcag gggattgcgg gagcagttta
     2821 tccagatgtg cggtaagcgc ggcgatatcg gcatcaacat cctcttcgaa tagggagcgt
     2881 tgcataccag ccagcgtttc acattttttt ccgaagcgct gttgacgaac cagtttcagc
     2941 atttcttcca gaagctggat gcgccggctt ttctccagta attcccgctc tttttcggca
     3001 ttctccgaca tgacttcttg taccatggca agtgccatag cacgcagttt ttcgatgtca
     3061 ttcgtggtgt tgagagcgga gatatccata caggggagta taccactcca gatacggata
     3121 ttcattattt ttcactgagt tagcagtgtg tttttcgtgt tttcagggcg cagttcaggt
     3181 tcattctgcc agacccattt cgtcaggtca tgtcccttaa cctgctgcca gtcaacaccg
     3241 gcaatcagcc agttaaactc gtcgggagtg aggtgccagg cagcgtcatt tgcacgtggc
     3301 cagcggaagc tgcctttgtg cagacggcgg gtacacaacc acaccccgtg tttatcccac
     3361 cggagaactt tgatacgcga acggactttg ttaacaaaga cgaaggcggc tccctcatgc
     3421 catgtcgacc ggagttcatc ctgtatatac tgcgtcagtg agtcgatgcc ccggcgcatg
     3481 tcaacaggtt taattgcgat gaagacatta tcaggactga gcatatttga gtgccctgaa
     3541 gacatcggta agttgtgacg gctgacagca catccgtact ccaccgggaa ggaagagcgt
     3601 gactggctca ttcgtgacgg gctgtggtac atcagcatgc agtggtggcg caatatgaac
     3661 gggaagaacg gtcggttcat ttgcccggcg ttcagctttt gcgacatcct gcggccattc
     3721 gcgaagtgat gtgaagggaa tatcgttgag ttcacagtat tgctgacgtg taagaccact
     3781 ggcgcgccag gcagcaacgt gctgcctttt ctgttcaatg gtccatcggg gttttgacat
     3841 cgattatctc cggtaagttg gtgaatggat gtcggtaata ctaactgacc gaagatccct
     3901 gtgaagatga cttccccgta acgacactca aaatttcggc aaattcacag tcattccagt
     3961 cggagatcca gcgggcgtcc cgtatgggca gtgaatatta ccggaccctg cagaatggcg
     4021 gacgtcatgc cgctgctgcc gcacgggaac agcgccgcgc ccttgcagaa ctgaacagtc
     4081 agttgacgga aattcgcggt tctgctgtcg gaatggctgg cgcatttgcc ggtgcctttg
     4141 ccaccggaca cctgatttcg ctggccgatg agtggggttc cgtgaatgcc cgtctgaaac
     4201 aggcatctca gtcatcggat gaattctcgt catcacagaa agtgctgatg gatatcagtc
     4261 agcgaacagg taccgcgttt tcggataatg cggccctgtt tgcccgttcg gctgcctcga
     4321 tgcgtgaata tggttacagt gctgatgatg tgctgaaggt gacggaggcc atttcgacag
     4381 gactgaaact gtcaggggct ggagttgcgg aatccggttc ggtgatcacc cagttcagcc
     4441 aggcactggc acagggtgta ctgcgtggcg aagaatttaa tgctgttaac gaaaatggcg
     4501 accgggtgat ccgcgcgctt gctgcgggga tgggtgtggc ccgtaaagat ctgaaggcaa
     4561 tggcggatga cggaaaactg acagcggata aagtggtccc tgcgttaatc agccagctgg
     4621 ggatattacg tgatgaatat gcggccatgc cggaaacggt ttccggtagt atcacgaagg
     4681 tggaaaacgc ctttatggcc tgggtgggcg aagtgaatga ggcctgcggg gtgacaaaaa
     4741 cgctctccgg cgtgctgaac ggtgttgccg gacagattga taatgtggca acagctgtgg
     4801 gggcgctggt tgccgtcggg gttgcccggt actttggcaa tatggcctcc ggagcgatgt
     4861 ctgccacggc aggacttgtg acggctgcac gtaatgaagt tgcactggcg gaagcacagt
     4921 tcaggggaac gcagattgcc acggcgcggg caagggcagc cgtgtaccgt gctcagcagg
     4981 ccgtggcggc agcccgcggg acggagatgc agattgctgc agaggcccgt ctggcggcca
     5041 cacaggaacg cctgaacaga aatattgctg ccagaagcgc cgcccagaat gcgctgaaca
     5101 gtacaacggc ggtgggctca cgtctgatga gcggtgcgct ggggctggtt ggtggcgtac
     5161 ccggactggt gatgctgggg gctgcagcat ggtacacgct gtaccagaat caggagcagg
     5221 cctgggagtc tgcgcgccag tatgcactga cgatagatga aatcgcgcat aaaacgccgt
     5281 caatgtcttt gcctgaagcc tcagataatg aaggacgaac acgggcggcg ctgacagagc
     5341 agaaccggct gattgatgaa taggctagtc gggtgaaatc cctgcaggaa aaaatcgcag
     5401 gatatcagta tgttctggcg aacccgggct ggacgaccgg tgacggattc atgataaacc
     5461 atctgacctc ggtgaagacc gtaacggaag ggcttgctca ggcaacagag cagcttgccg
     5521 ttgagcagtc ccgtctggca ctgatgcagg aaaaagcgca gtccattcag gatgtgcttg
     5581 ccgggctgga agaccgtcgt gtggcgttaa ttcgtcagca ggcggcagag cagaataagg
     5641 tgtaccagtc catgctggtt atgaacggtc agcatacgga attcaaccgt ctgctggggc
     5701 tgggtaatga actgcttcag cagcggcagg gactggtgaa tgtgccgtta cggctgccac
     5761 aggccactct ggatgataaa cagcagagtg ccctgacaaa aacagagcgt gagctggccc
     5821 tgtccagact gaaaggggaa gaaaaagagc gtgtccgact ggggtatgcg gcggatgacc
     5881 tcggttttgt gggtgatccg tatcaggagg cgagacaacg ttatatcagt aatgccctgg
     5941 aagcctggcg caataacgag gcgaataaac ccaaatcccg gggtggaaaa tcagagacgg
     6001 aaaaagcgga agacagtttt tcccggctgc tgaagcagca gaaagagcaa ctggcactgg
     6061 cggggcagaa tacagagctg gcgaagctga aataccagac tgcgcagggc gaactgaaaa
     6121 ccctgacgga gatgcagaag caggaactgc tgcgcaatgc ggccctgatt gaccagcaaa
     6181 aaatccggga gcagttgcgg tcccgggaag agaccctgaa gaatgataat gtggctgctc
     6241 gtgcatcaaa tgaagcggaa ctgctggggt acgggcaggg agagcggctc cgtgaacgca
     6301 tgcgggagtt gcagcagatc cgcgacagtt tccgccagaa ggatgcggac cttcagtctc
     6361 agtatcagac cggggatatc agtgaggatt tttacagaca ggctctggca cagaacgcgc
     6421 agtatctgag tgaacgtctg aaagagcagg aagcctttta tgccgaatcg gatgtgcagc
     6481 gtgcggactg gcagaaaggg ctgcaggagg gattcagtaa ctgggtggat aatgcgtccg
     6541 attacgcctc acaggcagca cagcttgcga cggagggtat ctcagggatg gtgaataaca
     6601 tcacggagat gctgaacgga aataaagtgg aatggcgcag ctgggcctca tcagtgctgc
     6661 aggaaatatc aaaagttctt atgaatgccg cgattgtcaa cgggatcaag acggcggcaa
     6721 acagtatgtc cggagcggga ggatttatcg gcagtattgg tagctggctg ggcggtgcgg
     6781 tggccaatgc aaaaggcggt gtgtatacct cggcaaacct gggtgcgtac agcaacagta
     6841 ttgtggatac gcccacgtac tttgcctttg caaaaggggc gggactgatg ggggaggccg
     6901 gtcctgaagc cattatgccc ctgacccggg cggcggatgg ctcgctgggt gtgcgagcgg
     6961 tgggcagtat gaacggcagt gcaggtctgg tgtattcccc ggtctaccat atcgccattc
     7021 agaatgacgg gactaatggc cagatagggc cggaagctgc gggcagcctt gtgcaactga
     7081 ttgaccagcg ggtgcaggcg gtgatgctgt ccatgcgacg tgacggagga atgctgagtg
     7141 gctgagataa aaacgctgca tctggtcccg cgtgaaggga tgcaggtgag tgagaaaccg
     7201 tcggtggcga gggtacggtt tggtgacggt tatgaacagc gccgcccgac gggacttaat
     7261 gcccgactga agacgtttca ggcggtgttc cgggtgacgg atgaggcgac ccggcgatgg
     7321 ctggaagagt ttttatcgtg gcatggtggt taccgtgcct ttttgtggcg accgccgaaa
     7381 cataaccgga cggtgagggt ggtatgccgg gagtggagcg tcacagataa cgccaggtac
     7441 agtgatttca gttgtacgat tgagcaggtg gtgaactgat gcaggatatt cacgaagaaa
     7501 gtctgagcga gtcggttaaa tcagagcagt caccgcgggt ggtactctgg gaaattgacc
     7561 tgacggtgca gggtggtgag cggtattttt tctgtaatga gctgaatgaa aaaggggagc
     7621 cggtcacctg gcaggggcgt aagtatgagg catacccgat tgacggcagc ggctttgaga
     7681 tgaacggccg gggcagcagt gccagaccgt cgctgacggt gtccaatctg ttcggtctgg
     7741 tcaccgggat ggcggaagac ctgcagagtc tggtgggggc cacggtggtc cgccgccggg
     7801 tgtatgcccg ttttctggat gcggtgaatt tcgttgcggg caatccggag gcggacccgg
     7861 agcaggagct gagtgaccgc tgggtggtgg agcagatgtc gcagctgaca gccatgacgg
     7921 cctcgtttgt gctggctaca ccgaccgaga cggacggggc gctgtttccc ggtcgtatca
     7981 tgctggcgaa cacctgtatg tggacctacc gctctgatga gtgtggttac acgggcgggg
     8041 ctgtggcgga tgagttcgat aaacccacca cggatatccg taaggacaga tgcagcaagt
     8101 gcatgcgcgg gtgtgaactg cgcaggaatg tcggcaattt tggcggtttc ctttccatta
     8161 ataaactttc gcagtaaatc ccgatttatg acacagactg aatcagcgat tctggcgcat
     8221 gcccggcggt gtgcgcctgc ggagtcgtgc ggcttcgtga taagcacgcc ggagggggag
     8281 cggtatatcc cttgtgtgaa tatttctgca gagccggagg cgtattttcg tatcgcaccg
     8341 gaagactggc tgcgggcaga gatgcagggg gagattgtgg cactggtcca cagtcatccc
     8401 ggtgagctgc cctggctgag cgaggctgac cggcggctgc agataaaaag cgcactgccc
     8461 tggtggctgg tctgccgggg ggaaattcat aaattccgct gtgtgccaca tctgacagga
     8521 cggcgctttg agcacggggt gacggactgt tacacgctgt tccgggatgc ataccatctg
     8581 gcgggaattg atatgccgga ttttcatcgc gaggatgact ggtggcgcaa cggccagaac
     8641 ctgtacctgg acaatatggc ggtcaccggc ttttaccggg tgcccctgtc ctctgcaccg
     8701 ccgggcgata ttctgctgtg ctgctttggt gcttcggtac cgaaccatgc caccatttac
     8761 tgcggcaacg gtgagctgct tcaccatctg cctgaacaac tgagtaaacg ggagaggtat
     8821 tccgaaaaat ggcaacgacg aacgcattct gtctggcgtc accgccactg gcacgcatct
     8881 gccttcacgg ggatttgcaa cgatttggcc gccgcctcag cctgtatgtg aacacggcag
     8941 cggaagccat tcgcgccctg tcgatgcaga tgccgggctt tcgccgtcag atgaacgaag
     9001 gctggtacca gatacgtatt gccggtgatg acacggcacc ggaggcggtg tatgcccgtc
     9061 ttcacgaaca gctgggtgag ggaacggtca tccacattgt gccgcgactg gccggggccg
     9121 gaaagggtgg actgcagatt gtgttggggg cggcagccat cgtgggctct ttcttcactg
     9181 ccggggcatc aatggcgtta tggggttcag ccctggcagc cggtggtttt tctgccacca
     9241 cgatgctgtt ttcacttgga gccagcatga ttctgggcgg tgtggcccag atgctggccc
     9301 cgaaggcaaa aacaccggat taccgcgcaa cggataacgg cagacagaac acgtactttt
     9361 cctcgctgga taacatgatt gcccagggga acccgatgcc ggtgccttac ggggaaatgc
     9421 tggttggctc ccgccgtata tcccaggaca tcagcacccg tgatgaaggc gggggcggaa
     9481 aggtcgtggt tatcgggcga cagggataaa acataaaaaa atcccgcagt gatcgcggag
     9541 ctgcggggac agacaaatga agatcaatgt gaaggagttg tttttgttac tcgggcaaaa
     9601 aaacactaac gcagcgaaat tataagcgcc acagtcagtg tgtgaaaatg tgaagatatt
     9661 cagaaatttt attccgtcat gacgcaggca cccggtgagg tgcctgttgt ttttgtgagt
     9721 gaacaattat cacggtaaga ggtgatgtaa tgggcaaagg tggcggcagg gcgcacacac
     9781 cgcgtgaggc gaaagacaat ctcaaatcca cgcagatgat gagcgtgatt gatgcgattg
     9841 gtgagggacc gatagaaggc ccggtgaaag gcctgcagag tattctggtg aacaaaaccc
     9901 cgctgacgga cacggacggt aatcccgtga tacacggtgt gaccgcggtc tggcgtgccg
     9961 gggagcagga gcagacacca ccggaaagct ttgagtcctc cggctctgaa actgtactgg
    10021 gtgtcgaagt gaccagggca aaaccggtaa cacgcaccat tacgtcagcg aacattgacc
    10081 gcctgcgggt gaccttcggg gtgcagtcac tggtggagac cacgtcaaag ggtgaccgta
    10141 atccgtcctc tgtccgtctg ctgattcagt tacagcgtaa cggtaactgg gtgacagaaa
    10201 aggatgtcac cattaacggc aagaccacct cacagttcct tgcgtcggtg attctggata
    10261 atctgcctcc ccgccccttt aacatccgga tggtcaggga gacggcggac agcaccacgg
    10321 accagctgca gaacagaacg ctgtggtcgt catacaccga aatcatcgat gtgaaacagt
    10381 gctacccgaa cacggccatt gtggggatgc aggtggatgc ggagcagttt ggtggtcagc
    10441 agatgacggt gaactaccat atccgcggtc gcatcatcca ggtgccgtca aactatgacc
    10501 cggaaaaacg cacgtacagt ggtatctggg acggcagtct gaaaccggca tacagcaaca
    10561 acccggcctg gtgcctgtgg gacatgctga ctcacccgcg ctacggcatg ggaaaatgtc
    10621 tgggggcggc ggatgtggac aagtgggcgc tgtatgccat cgggcagtac tgcgaccaga
    10681 cggtcccgga tggtttcggg gggaccgagc cgcggatgac ctttaatgcg tacctggcac
    10741 aacagcgtaa ggcgtgggac gttctcagtg atttctgctc tgcgatgcgc tgtatgccgg
    10801 tatggaacgg tcagacgctg acgttcgttc aggaccgccc gtcggatgtg gtgtggccgt
    10861 acaccaacag cgatgtggtg gtggatgata acggcgtggg attccgctac agcttcagtg
    10921 ccctgaagga ccggcacacg gcggtggagg tgaattacac cgacccgcag aacggctggc
    10981 agacctccac ggaactggtg gaagacccgg aagccatact gcgctacgga cgcaacctgc
    11041 tgaagatgga tgcgtttggc tgtacctgcc gcggtcaggc tcaccgtgca ggtctgtggg
    11101 tgataaagac cggactgctg gaaacgcaga cggtggactt cacgctcggg tcacaggggc
    11161 tgcgtcacac accgggtgac attattgaaa tctgtgataa cgactatgct gggactatga
    11221 ccggcggacg tgtcctgtcc attgatgctg ccacccgcac cctgacgctg gaccgtgagg
    11281 ttaccctgcc ggagacaggt acatcggcgg tgaacctgat taacggcagt ggtaagccgg
    11341 tgagtgtgga catcaccgca caccccgcgc cggaccggat acaggtcagt accctgcctg
    11401 atggtgtgga gacatacggg gtgtggggac tctccctgcc gtcactgcgc cgtcgcctgt
    11461 tccgctgtgt ctccgtccgg gaaaacacgg atggcacctt tgccatcacg gcggtgcagc
    11521 acgtaccgga aaaagaagcc atcgtggata acggtgcccg ctttgagccg cagtcaggct
    11581 ccctgaacag cgtcatccca ccggcagtgc agcacctgac ggtggaggtg agcgcagctg
    11641 acggccagta tctggcacag gcgaaatggg acacgccgcg ggtggtgaag ggggtgcgct
    11701 tcagtctgcg actgaccagc ggaagcggag aagacagccg tctggtgacc accgctatca
    11761 ctgcggatac agagcatcgt ttcagtggtc tgccgctcgg ggaatacacc ctgacagtca
    11821 gggcaattaa cagttatggc cagcagggcg aaccggccac caccaccttc cggattaacg
    11881 cgccagcaaa acccgccacc attgaactga cgccggggta ttttcagata acagcggtac
    11941 cgcgtcttgc ggtgtatgac ccgacggtac agtttgagtt ctggttttcg gaggcaaaaa
    12001 tcgcagacac atctcaggtg gaaacctctg cccgttatct ggggaccggc agtcagtgga
    12061 gtgtatccgg cccgcacatt aagcccggga aggatttctg gttttacgtg cgcagcgtca
    12121 acctggtggg gaaatctgct tttgtggagg ccagtggacg ggccagcaat gatgcagaag
    12181 ggtatctggg gctgtttcgg gaaaaaatag gaaaactgca tctggctcag gggctgtggg
    12241 agctgataga caacagccag cttgcggatg agatggcgga gatgaagacc accatcaccg
    12301 aaacccgcaa tgaaatcaca cagacggtca gtaaaacgct ggaggaccag agcgccacca
    12361 tacagcagat acagcgcgtg cagaaggaca caaatgatga ccttgctgca ctttacatgc
    12421 tgaaggtaca gaaaacaaaa aatggcatac cctatgttgc cggtattgga gcggggattg
    12481 aggatactga tggccagccc ctgagcaaca tactgctgct ggctgaccgt attgcgatga
    12541 ttaacccgga ggacggcaac accacgccgt tatttgtggc gcaggggaat cagttgttca
    12601 tgaacgatgt gttcctgaag cggctgtttg cggtgagtat cacctcgtcc ggcaatcccc
    12661 cgacgttttc cctgacgccg gagggcaggc tgaccgcaag aaatgctgat atcagcggta
    12721 acgtgaatgc gaactccggg acgctcaaca acgtcacgat taacgagaac tgccgggttc
    12781 tgggaaaact gtccgcgaac cagattgaag gcgatctcgt taaaacagtg ggtaaggctt
    12841 ttccgcggga ctcccgggca ccggagcggt ggccatcagg gaccgttacc gtcaggattt
    12901 atgacgatca gccttttgac cggcaaattg ttattccggc ggtggcattc agcggcgcta
    12961 agcatgagag agagcatact gatatttact cctcatgccg tctgatagtg cggaaaaacg
    13021 gtgctgaaat ttataaccgt accgcgctgg ataatacgct gatttacagt tgcgttattg
    13081 atatgcctgc cggtcacggt cacatgacgc tggagttttc ggtatcagca tggctggtga
    13141 ataactggta tcccacagca agtatcagcg atttgctggt tgtggtgatg aagaaagcca
    13201 ccgcaggcat cagtatcagc tgaattttat aacccatata cgggcgccag aaatggcgcc
    13261 ttttttattg cagaaaagcg agaggtaatt atgcgtaaag tttgtgcagc cattttgtcc
    13321 gcagccatct gtctggctgt atccggtgcg cctgcatggg cgtctgaaca tcagtccaca
    13381 ctgagcgcgg ggtatcttca tgcccgtacg aacgctcccg gcagcgataa tctgaacggg
    13441 attaacgtga aataccgtta tgagtttacg gacgcgctgg ggctgattac gtccttcagt
    13501 tatgccaatg ctgaagatga gaaaaaaacg cactacagcg atacccgctg gcatgaggat
    13561 tccgttcgta accgctggtt cagcgtaatg gcggggccgt ctgtgcgcgt gaatgaatgg
    13621 ttcagcgcgt atgcgatggc gggtgtggct tacagccgtg tgtcgacttt ctccggggat
    13681 tatctccgcg taactgacaa caaggggaaa acgcacgacg tgctgaccgg aagtgatgac
    13741 ggtcgccaca gcaacacgtc tctggcgtgg ggggctggcg tgcagtttaa cccgaccgaa
    13801 tccgtggcca ttgatattgc ttatgaaggt tccggcagtg gcgactggcg cactgacggt
    13861 ttcatcgtgg gtgtcggcta taaattctga ttagccaggt aacacagtgt catgacagcc
    13921 cgccggttca ggcgggcttt tttgtggggt gaatatgaca gtaaagattt ctggtgtact
    13981 gaaagacggc acaggaaaac cggtacagaa ctgcacaatc cagctgaaag caaaacgtaa
    14041 cagtaccacg gtggtggtga acacgctggc ctcagaaaat ccggatgaag ccgggcgtta
    14101 cagtatggac gttgagtatg gtcagtacag cgttattctg ttggtggagg gattcccgcc
    14161 gtcacatgcc gggactatca ccgtgtatga agattctcaa cccggtacgc tgaatgattt
    14221 tctcggtgcc atgactgagg atgatgcccg tccggaggca ctgcgtcgct ttgaactgat
    14281 ggtggaagag gtggtgcgta acgcagagga ggcgaagaag aatgccggag aggcggagac
    14341 gtcagcgagg aatgccggca tatcagccag tcaggcagaa gagagcgctg caaatgctga
    14401 cacttcagca ggggatgcat cggagtcagc ccggcaggcg gcagaaagtg cagcctcagc
    14461 aaagcagtca gaggaggcgt cctcgtcctc ggcctctgcg gccgctcaaa aagccagtga
    14521 gtcattacaa agtgcaacag atgctgagtt gtcaaaaaag acggcagaaa gtgcagccgg
    14581 taatgcagtc agggatgcaa cgaccgcaac agaaaaagcc cgggagtcag cagaaagcgc
    14641 acagtcagcg gaacaaagca ggatagcggc ggaagaggcc gtaaaccgaa tccccaccgt
    14701 ggtgggacct cccgggccaa agggggaacc ggggcccgcg ggtcctcagg ggccgaaggg
    14761 agataaagga gagcgtggcg acaccggccc ggcaggggcc aaaaggtgac aaaggcgatc
    14821 cgggagtggc tggaccagca ggtccggcag gtgcgccggg gccgaaaggc gataaaggtg
    14881 atccgggagt agcaggtcca gcaggtccgg cggggccaca gggaccgaag ggagacacag
    14941 gagcccccgg gcaaggaaca gaactgctta ctactgccaa tacatggact caggcacaaa
    15001 cttttaatgg tggtattaat ggcaatttga cggtgaccgg aaacggctca tttaacgata
    15061 ttcagatccg ttcggataaa cgcaacaagc gaaatctggt aaaactggat aatgcgttag
    15121 atcgtctgga ggcacttact ggttatcttt acgagataca gtactctgcc gacggttggc
    15181 aaacgtcggt tggtttaatt gctcaggatg cacaaaaagc attgcctgaa ctggtaactg
    15241 aagacgcaga cgttatatct ggtgaaaaac gtctgcgtct taactacaac ggcataattg
    15301 cattgttagt cgagggcttt aaaacacttc gtcatgagat taaagaactc cgggagaagt
    15361 aaacgacagc tgttgtagtt tctggtttct actgaattta aattgtgggg tgacactcac
    15421 cccacgcatt cagaaggggg agatgagatg ggggtaacat cgggatgggt aggctcttcg
    15481 gctaagagcg aaacaggtga gcagtggatg ggagccgccg gaaccaaact gggactgagt
    15541 aaacctttta tgatgagcca aatggttggg cgaactatgg gctgtaaaat tgcaactgag
    15601 tactataaat ggaaatcctc tgacaaggtt gataactggg gcgcagttgg cgctgactgg
    15661 cctttagaag aaaaaagtaa aggtacaatt acaaacgccg caagctgcgg atcagggagg
    15721 ctggtagggg ctgtcgttac actttctcac tttttgacga actctacacc gacagctgct
    15781 gtttatttag ccggtggtaa agcaggtaac atcaccgtaa acgtaggtgg tgctacacaa
    15841 accatgattt atcagggcgt tgttagtggg ttccagtatt actggtcagg ttctgttagt
    15901 tccgctttcg tggaggcaat gaaaaagacg ggagtacccc aggatctaaa aattagttaa
    15961 atggtaaata aataatttta aaaacttcac gttctacata ccggagacgc cggatatata
    16021 ggatattgtt ttaagttgcc agagaaattt ttccggacgg atgctgataa taatgatgtg
    16081 ccaatgacgg cagcatcatg gatggcactg agtgaagcga cagagcaggc gatgtttgcg
    16141 aagggagtgg agattaatac acggcagttg cagatgaaag ccgaggttga ggcgctgaca
    16201 gacctgaagg cgatccgcag ttatgttgtc ggatggcctg ctggctgaat aaaaaaacgg
    16261 gaccacgacc agtcccggaa ccatgagttt tagggtatta gtttgttatc atagttagcg
    16321 tgctaagtat gccatgtcag gttgattagt gaagtgatgt tgttcgcatt tttgcacggc
    16381 ggagaatatt cagatttttg gaaatccata tttttcccgt gcgcgattac atgcttcatt
    16441 tacgatgcta ttttcgccgg acattgcgaa ttccctgcat gtggacggtt tttgtaaaca
    16501 gagcaatatg cgttttctcc gggggggccg gcaagagcgc cacatcgggg atttttctga
    16561 ttggtgccgc gcatacagcg atgaaaagga gatatttgct cagtgagact gaccgaaacc
    16621 tttccgccag catcgtcagc ttctgcccag taaaaagaga cgcggaaaat gcacaacagg
    16681 caccacacgt catgcatgga ttcagattgc tcataattca ccagtacagc tataaatcgt
    16741 aaagagaaca gcggtacatc gtatgtaaga acgtaccgcg gctggctggt gaacttccga
    16801 tagtgcgagt attgaatgat ttccagccgt taccgatttt acgtgctaat tagtgaacaa
    16861 accactcgtc agcagactcc caggtatctt tcagagtttc ctgaacaaaa gttttagctg
    16921 aatctttatc ggcggtgcgc gtaacagaaa ggccatcgtt gctggtagct tttacgatca
    16981 cctctacatc gtcataacgt ttactgatgc gtcgggttaa ttcttccttt aacgcatcca
    17041 cagcaccggt tggcatttta gtcatttttt ctttggctat gcagatttca atacgcataa
    17101 aagtccctct acactgtgtt tgtatacagt atcattttta actgtatgga taaacagtat
    17161 taagggtgaa ggaaagtgat tttttggggc atgcatgggg caaaaatatt tgttgtgggg
    17221 cattttgggg catgattggg acactttgtt acatatgaac tttgccgatt tttatatgac
    17281 atggaaatac aacgctttga ttcttctaaa taaaaaacat gctcttgggc gttctttagt
    17341 gatttttaaa aattccgtat cacgcagtta aagtggcggg catactcttc aaggctggtg
    17401 atccccaacc gcacccattt aggatgcgac cattgcggta gaccgatgta aatcataacg
    17461 cgtgaaggat ctcttccacg ctacgcacac gggcgatgcg cgggtagata tgattaatgc
    17521 tgttattgtg ttgctcggcg ctggcggcac tacaggcgtc ttcggcaatc accagattaa
    17581 agccaagttc ccaggcattg cgggcggtgg attcaacacc gatattggtc gagatcccac
    17641 ataacactat tgtatcgata cctcggcggc gcagttgcaa ctccagatcc gtaccgtaaa
    17701 acgcacccca ctgacgtttg atgatttcga tatcactgtc agttgcacct aatgcagctg
    17761 gatgttgcca ccagttttcg ggcaacactt tag
//
LOCUS       DAJVUJ010000082        17728 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.082, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000082 DAJVUJ010000000
VERSION     DAJVUJ010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17728
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.082"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..538
                     /locus_tag="OQQ48_002462"
     CDS             <1..538
                     /locus_tag="OQQ48_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="involved in the stability of the curlin proteins
                     during assembly; involved in the secretion of the major
                     curlin subunit CsgA across the outer membrane; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HCS1953197.1"
                     /translation="GLQNLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSII
                     GYESNVKSGGVGARYFGIGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEV
                     QAGVFRFIDYQRLLEGEVGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAE
                     RQNDILVKYRHMSVPPES"
     gene            complement(602..1093)
                     /locus_tag="OQQ48_002463"
     CDS             complement(602..1093)
                     /locus_tag="OQQ48_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HCS1953198.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(1195..1749)
                     /gene="ycdY"
                     /locus_tag="OQQ48_002464"
     CDS             complement(1195..1749)
                     /gene="ycdY"
                     /locus_tag="OQQ48_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HCS1953199.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWMEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(1773..2510)
                     /gene="ycdX"
                     /locus_tag="OQQ48_002465"
     CDS             complement(1773..2510)
                     /gene="ycdX"
                     /locus_tag="OQQ48_002465"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HCS1953200.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMALIAEFADL"
     gene            complement(2565..3503)
                     /gene="ghrA"
                     /locus_tag="OQQ48_002466"
     CDS             complement(2565..3503)
                     /gene="ghrA"
                     /locus_tag="OQQ48_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HCS1953201.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHWEDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            3737..3824
                     /locus_tag="OQQ48_002467"
     tRNA            3737..3824
                     /locus_tag="OQQ48_002467"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3771..3773,aa:Ser,seq:gga)
     gene            3869..4005
                     /locus_tag="OQQ48_002468"
     ncRNA           3869..4005
                     /ncRNA_class="other"
                     /locus_tag="OQQ48_002468"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(4096..4782)
                     /locus_tag="OQQ48_002469"
     CDS             complement(4096..4782)
                     /locus_tag="OQQ48_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HCS1953202.1"
                     /translation="MSFMAFVTCLTIAFIAGFAVARKLTLRKVASSVIIAKSQPVPSS
                     ATSPAAKASTRSYIPQKTYRDSRWQERDELFQGMVAEGILLERKEWLITRCEKMYFDN
                     LEKWFGHYCHIHCQVSLGRLINFPEQPEFSEEERKRFFAIYNGMAMDYVLVSKKNNKI
                     VCVIELDDPSHERADRIIRDRWLDTIMTLARVPFVRASVTKLEEIPPVWETRDKIRPL
                     VAATKSEPVH"
     gene            complement(5363..6151)
                     /gene="phoH"
                     /locus_tag="OQQ48_002470"
     CDS             complement(5363..6151)
                     /gene="phoH"
                     /locus_tag="OQQ48_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HCS1953203.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(6773..8044)
                     /gene="efeB"
                     /locus_tag="OQQ48_002471"
     CDS             complement(6773..8044)
                     /gene="efeB"
                     /locus_tag="OQQ48_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HCS1953204.1"
                     /translation="MQYKDENGVNEPSRRRLLKVIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFSLPGVKDANDYLGSALLRV"
     gene            complement(8050..9177)
                     /gene="efeO"
                     /locus_tag="OQQ48_002472"
     CDS             complement(8050..9177)
                     /gene="efeO"
                     /locus_tag="OQQ48_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HCS1953205.1"
                     /translation="MTINFCRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITAQAEDLAQLRGVLGLD"
     gene            complement(9235..10063)
                     /locus_tag="OQQ48_002473"
                     /pseudo
     CDS             complement(9235..10063)
                     /locus_tag="OQQ48_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
     gene            complement(10732..12240)
                     /gene="putP"
                     /locus_tag="OQQ48_002474"
     CDS             complement(10732..12240)
                     /gene="putP"
                     /locus_tag="OQQ48_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HCS1953206.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKHASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQES"
     gene            complement(12399..12608)
                     /locus_tag="OQQ48_002475"
     CDS             complement(12399..12608)
                     /locus_tag="OQQ48_002475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1953207.1"
                     /translation="MMLQLCATMLNVTCVASLKMNEMLIKEIDMTGIKKITQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            12663..16625
                     /gene="putA"
                     /locus_tag="OQQ48_002476"
     CDS             12663..16625
                     /gene="putA"
                     /locus_tag="OQQ48_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HCS1953208.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNCNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLENLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLVDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTYETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQCNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVVDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELHKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGIQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(16665..17303)
                     /gene="rutR"
                     /locus_tag="OQQ48_002477"
     CDS             complement(16665..17303)
                     /gene="rutR"
                     /locus_tag="OQQ48_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HCS1953209.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFIIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            17591..>17728
                     /locus_tag="OQQ48_002478"
     CDS             17591..>17728
                     /locus_tag="OQQ48_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HCS1953210.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     AL"
BASE COUNT         4452 a         4780 c         4347 g         4149 t
ORIGIN      
        1 gggcttacaa aacctgctta acgagcgcaa gattattcgt gcggcacaag aaaacggcac
       61 ggttgccatt aataaccgaa tcccgctgca atctttaacg gcggcaaata ttatggttga
      121 aggttcgatt atcggttatg aaagcaacgt taaatcgggc ggtgttgggg caagatattt
      181 tgggatcggt gcagacacgc aataccagct cgatcagatt gccgtgaacc tgcgtgtcgt
      241 caatgtgagt accggtgaga tcctctcctc ggtgaacacc agtaagacga tactttccta
      301 tgaagttcag gccggggttt tccgctttat tgactaccag cgcttgctgg aaggggaagt
      361 gggttacacc tcaaacgaac ccgttatgct gtgtctgatg tcggctatcg aaacaggggt
      421 cattttcctg attaatgatg gtatcgaccg tggtctgtgg gatttgcaaa ataaagcaga
      481 acggcagaat gacattctgg tgaaataccg ccatatgtcg gttccaccgg aatcctgaca
      541 gataaaaaag ccggaattat catccggcat tttactgttt aagcgaaagc gtgagtgccc
      601 ctcacgcttt atttttgatt tcctgctcac ggtgagccgt ctttgcacta cgcgccgcca
      661 gccacaggcc gctgtttttc attgcgtaac caaataccag cccaagcgcc agcgaaggta
      721 acaccagttt ccagtcaccc tgcccggcaa acgtcgcgca tgcgcctata aacgtccccg
      781 gaacaaatga aagcagcagc tgtttggcct gaatacacat cagaaacgcc acaataccgg
      841 ttatgacata accgagaatt tccagatgtg gtgccagtgc actaccgtaa ataatgacca
      901 tcgcccacac cacgccacta agcagggttg cagcggagat cgccagccct ttcagcccac
      961 cttgcgggca ggcaaagtag gccgtacagc ccaggaagcc cgcccagctt agtaagccca
     1021 gggaaacagc cacccatccc cagataccgg agagaatgcc tgttgtgatt gcaatagaga
     1081 gaagtatgtt catgccgcgc atgatagcag aaaacgcggc catgaatgag atcgagcaca
     1141 cattttatgc aacaacaaat tatctgttgc agttatgagt gacgatgatc acatttactc
     1201 ttcagaatct tcttccagct cgtcccacat tgcactaatg gcatcacggg ttagcggtgc
     1261 catggtgcgc cagaaaggcg tggttgcatg ggcctccact ttgccgagga acgcaccaca
     1321 ccagggtaac agatactcac tgaacagtgt ttccagtgct tcgctctcat cttccgttga
     1381 ctgatcttcc atccaggaag ccgcaagcag caatgtgccg atgtgatcgg ctggcgtatc
     1441 cgccagtggc atccctcgtt cggaaagaaa agcgcgcact tccgcttccg ttgcgtcctc
     1501 aacccatgcg ctacgatatg gcggcacggc acattcatcg ccgataaaca acgcattgta
     1561 atcggcagag acttgcgcca tatcacagct tttctgtaaa cgcgtcagta actcatcctg
     1621 ctccagtggc cagttcgcag ccagtttccc ctcacgaatc agggtaaaca gcggcaccag
     1681 taaaggatct tgcggttggc ggtaatagag cgaacccagc acacgacaga ggatagaaaa
     1741 ctcgttcatt aatatatttc cattactagt aattaaaggt ctgcaaattc cgcaatcagt
     1801 gccataccgc gagattcaag gaagttcagt aagcggcgcg gagaaacatt caaaatgcgc
     1861 tctggcggaa aatctaccgc gtcgaggatt ttaagacact cttcaaattc ccccatggta
     1921 aacgcggtgt gagaatccga gcctaatgcc acccaacctc cagcatcacg taccgctgcg
     1981 gctaccgcac gacagttgtc ttcactgccc ttacgtgagt gtaaaaatga agaattattg
     2041 atttccagcg ccacctgatg tttcgcggct gcttcagcaa cggctttcac atctatttca
     2101 tatttgggat ttccgggatg gcttattata tgcacattgc cgctggcgat agtggcgatc
     2161 atcgcttgtg tattggtcgc tttgtcatgt ggtgcaaaaa ccggctcatg aaaaccggca
     2221 ataatcagat ccagcgagtc aaacatttta ccgctgcaat caatttcacc atcaacattt
     2281 ttaatgttag cttcgatgcc gcgcaggatc cctaccccat caaccactcg cggccagata
     2341 cgcatgttaa tgaagtgcca gtgatgcgga gcatcttcca tatccgggcc atgatcggtg
     2401 atcgcaaaaa gtttaatgcc cttttgtttg gcctgggcaa tgtaatcact caatgtgcta
     2461 tatgcatgtg tgctggcaac ggtatgcata tgaaggtcga cgggatacat aactctctcc
     2521 tgtattcaat tcccgccaag gatagcagga atcctgatgc tttattagta gccgcgtgcg
     2581 cggtcgactt gcccgcagac cctctcccct ttttcgagct gggcaatggt gcgagaaatg
     2641 tactccacag cttcagcggg acgggtaatc gcggcgacat gtggtgttat cgtcacgcgt
     2701 ggatgttgcc agagcggact ttcaggcggt aagggttcac gattaaaaac atccaacatt
     2761 gcgcctttaa ctttgccgct atccagcgcc gcgagcaggt catcttccac aacatgaaca
     2821 ccacgcgcca ggttgaggag atacgcgcca tccggtaatt tttcgagtaa ttgttgatta
     2881 ataatgccga cggtttcagg ggtattcggt aacaaattaa tcaatacccg acattggctc
     2941 agaaatgcag acagttcttc ccgtccggca aagctttgca cgccaggcca cgatttacgg
     3001 gttcgactcc agcaacgcag cggaaagcgc caggtttgca gactctgagc aactttactg
     3061 cccagtacgc ctgcgcccaa aatgccgatg gtaaaatctt cccaatgata ttcaggcagc
     3121 ggttgccaat gcgaactatt ttgctggatg cgataatcgt caaaacgtcg aaaccaatgc
     3181 agcacctgac tgacagcata ttcctgcatt tgctcgccca taccagtatc ttccaggcga
     3241 aaaagtggaa cagaagggtt cagcatttca gggtgtgcct gtagcttgct caaaatagaa
     3301 tcaacaccgg ccccgagtgc gaacaccgct ttaagatcgc gccctgccag catttcaaca
     3361 ggaggatgcc agactaaagc ataatcagca gagtcattat ctccgctttt ccatgctctg
     3421 actcttgcct gaggaatagc tttgcgcagt gcctcaatcc accattgggt atcgaacgtt
     3481 gggtgataaa agatgatatc catactgact cccgaaaagc gttgtgcgaa atttattcgc
     3541 acttatcgtt atgatctaca aaggccacca gcataacaaa tccgtggtcg gtggcaaaaa
     3601 aagcagattt cgcttattaa aaccacacat tgattgaagt ttgaataaac gcgcgatttt
     3661 ttcaaaaagt ttgttgacct caggtcatga tttccctaaa ttagcgcccg ttccagcaag
     3721 acaggaacaa caatttggtg aggtgtccga gcggttgaag gagcacgcct ggaaagtgtg
     3781 tatacggcaa cgtatcgggg gttcgaatcc ccccctcacc gccatattta aagaagagct
     3841 cgtacgaaag tacgggcttt tttttcgtat attgcacaca ccggggggat gagaagcccc
     3901 gaccggggtt cgacaactgg cgacagccag ttggacagac cgtgaacgca gtgagcgggc
     3961 tgcccgcagg gcgagcgaag cgagtcaatc cccccctcac cgccatcttt aaagaagagc
     4021 tcgtacgaag gtacgggctt tttttcgtat attgcacaca ccggggggat gagaagcccc
     4081 gaccgggata cttagttaat gcaccggttc actctttgtc gccgcgacta atgggcgaat
     4141 tttgtcacga gtctcccaga ccggaggaat ttcctccaat ttagtgacag aagctcgtac
     4201 aaaaggcacc cgtgcaagag tcataattgt atccaaccaa cggtcacgaa taatgcgatc
     4261 cgccctttca tgactcgggt catccagctc aataacacag actattttat tgttcttctt
     4321 tgaaaccaga acataatcca ttgccatgcc attataaatt gcaaagaaac gctttctttc
     4381 ttcttcgcta aactcaggtt gttctggaaa gttgatcaag cgccccagag agacctggca
     4441 atggatatga caataatgtc caaaccattt ttccagatta tcgaaataca ttttttcaca
     4501 acgagtgatt aaccactctt ttctttccaa taaaatacct tcggcaacca ttccctgaaa
     4561 taactcatct cgctcttgcc aacgagagtc acgataggtt ttctgcggta tatagctcct
     4621 tgtcgacgcc ttagcggcag gggaagttgc agatgaaggg acgggttgcg atttagcgat
     4681 gattaccgat gaggcaactt ttcggagtgt caattttctg gcaacagcaa aaccggcgat
     4741 aaatgcaatt gtcaaacaag ttacaaaagc cataaagctc ataattaatt ttcacctatc
     4801 catgttgatt atatttcatt accaatgtta ctcgctgcag cgtgttacat gccaacaatg
     4861 caacgatcaa tatatagtta gtatcatgtt ttgattatta caaccatccc tatttagcca
     4921 ttgcggcttg ccttccacaa aaaaataaca acacacaata ttagcaaaga attaattaag
     4981 attaacctta catttataat attatcccta aagaataaat cagaggaata tattcggtgt
     5041 taaacagtca gtcaaagtat agcccattgt tgattaaacc tcataagcaa aattggcacc
     5101 agatctcctg tacaggttga atggccgaaa aaagataggc agatgctcat ttttttggct
     5161 tctggaagaa aaactctccg cgctggcagt attacactgg tctgatgcta tgacgaaaaa
     5221 tgcctggtaa gagtatctaa accagactcc agttcagctt atcaacacgc tcaaagtcgc
     5281 ccccctgaac gctaacctga agtgaaaatc tgttgaaata aaacaaagcc cggttcgccc
     5341 gggctctgca ccgataacac acttaactgt atgcatgcag cgtacgttgg caaagtgccg
     5401 aacgtacgca atcctctttt ccgaaccgga cgatcccaac catttcatct tcttcgaaac
     5461 gttccagcgc gtcacttaat ccggagcaca cgccgcgagg caaatcgcat tgcgtgatat
     5521 caccgttgac gataaccgtc acgttctccc cgaggcgggt taaaaacatt ttcatttgcg
     5581 cggcagtcac attctgcgcc tcgtcaagaa tgacgactgc attttcaaag gtacgtccac
     5641 gcatataggc gaacggcgcg atttccacct tccctatttc cggtcgcagg cagtactgca
     5701 taaaggaagc ccctaagcgc cggaccagca cgtcgtagac cgggcgaaaa tagggagcaa
     5761 acttttctgc gatatcacca ggtaagaagc caagatcttc atcggcttgc agaactggac
     5821 gggtgacgat aatcctgtcg acatccttat gtatcagggc ctctgccgct tttgctgcgc
     5881 tgatccaggt ttttccgcac ccggcttcgc ccgtggcgaa tatcagctgc ttactctcaa
     5941 tagccttcag atagtgcaat tgcgcttcat ttcgcgcgag gatgggcgag gtatcgcgac
     6001 tgtcgcgggc cataccaatg gcttctacgc cgctcatctg cacaagcgag gtgaccgatt
     6061 cttcttcacg ctgcttatgg ctgcgcgaat cccgtctcag cacacgtttt gcctcgcgac
     6121 gagctttgat cactgctttt tgtcttccca tggagagcac cttgagttgt ttgtattcat
     6181 cacacgcgcc gttggcagcg cgattatgcg cacgaacatc agagggttgg cttccttgta
     6241 agccatagtt tgcttttgga taaaatgccg aaaaacggct acgcgcaccg tttacggcgt
     6301 cggtaacaca tgaaaagaaa ggatgaggtt gaaaatgcaa agtgacgaga tgactaccgg
     6361 aggagaaaac tccgcgagtg gtggcgcgtt gattatctaa aacatgtcca gtacaggacg
     6421 ttaccatccg cgatctccat agtgactgac tatcactgcc gggaacttcc gctgctactt
     6481 aataagtaca acagatctcg cattttattg caacaatata tttacttata tttaactata
     6541 aaacactatt tcagtgacat tagtttctac tggaaagatg acagagtgat gacagtgatg
     6601 aaaaaagctg tgtgctttca gcaggatttg catcaggcat cagtgcacga tcgccggatg
     6661 cggcgtgaac gccttatcca gcctaccaaa cccgcactga ttgtaggcat gataagacgc
     6721 gcaagcgtcg catcaggcat ctgcgcacaa atgccttatc cgcctaaaaa cattaaaccc
     6781 gcaataacgc gcttccgaga taatcgttcg cgtccttcac tcccggcagc gaaaaaaaat
     6841 aaccgccgcc gataggttta acgtattcct ccagcgcttc gccattgagc cttttttgta
     6901 ctgtcaggaa gcctttttcc agatcgtgtt ggtagcagac aaacagcaac cccatatcca
     6961 gttgtccgga gttggtgacc cccaacgaat aactgtagcc gcgacgcagc atcaggctgg
     7021 actcactctc cgccgtgcgg ggattcgcca gccggatatg gctgtccagc gcgatcacct
     7081 tcccttccgg gtcgctggcg taatcaggca catcatgctc atgctgcatt cccagcggcg
     7141 caccggtttg cttatcacgg ccaaaaatcg tctgctgttc tttcagcggc gttctgtccc
     7201 aaaattccac tcgaaactga atcaagcgta ctgcctgata gctgccaccg attgtccacg
     7261 caggctcctg ctgatctgcc gttacccaca ccactttttg catcaactta tcattctggc
     7321 tatcgggatt ggcagtgccg tctttaaagc ccagcaaatt aatcggcgtc tctttgcctt
     7381 tactacgcgc tgcgtgatcg gaaataaacc cttcccgctt ccagcgcacg ctgagcaaat
     7441 ccggcgtgtg tttgatgata tcgcgcagcg catggatcac cgtgtcctgg gtgttggcgc
     7501 aaatttgtag caacacatca ccatggcata acgccgcatc cagcgagtcg ttggggaaac
     7561 gcgtcatctt ctgcaacttt ttcggcatct gtggcgcaag gccaaaccgc tcatcaaaca
     7621 atgagtgacc caccgataac gtgatggtga gattatcggg cgcaatgtag ccgccaagaa
     7681 tgccggaatc gagtggtggc aggcgcggat ttggcgtttc tggtgccgct ccgccctgag
     7741 tcagaaaagc aaaacgctga gtcaacaagc gaaacaaccg ttcaagatcg gctttatcgc
     7801 tggcaagcac atcaaacgcc accagcatca ttgcggcctg ttgtggcgtc aggatccctg
     7861 cctgatgctc accataaaac ggctgtttct cattgcgtgc atccggtgaa agcgtacccg
     7921 gcgcactttg cgttttttgt gcatgagcga ccggacaact tcccgccagc gccagcgcac
     7981 ctatcacttt cagtaaacgt cggcgtgacg gttcattcac gccgttttca tctttatact
     8041 gcataacgct taatccagtc ccagcacacc gcgaagttgc gccagatctt ccgcctgcgc
     8101 agtaatcggt cctttcagtg cattccggtc ggcatcggtc aatttgtcgt aggtttcaaa
     8161 accgtcttta gtacggtatt tcgccagaat ggtatcgacc tttttaaagt tggcatcgac
     8221 ttttgccagc agttccgggt tggctttttg cagttgtgga cgcagcaaat cgacaatttt
     8281 ctgcgagcct tcaacgttag cctggaaatc ccacagatcg gtgtggctgt agcgatcttc
     8341 ttcaccgcta attttgctgg ctgccacttc ctcaatcagt ccggctgcgc cgccgaccac
     8401 ttttgaaggt gggaaagcca gttcactgat gcgtttttgc aaatcgacca catctgtata
     8461 aagctgctca gcgtactgat ccatcccttt ggtggtgttg tcgccaaaca atgctttttc
     8521 cagacggtgg aaaccagtga attttgggtc ggcggctttt tgctcgtaat catcttcacg
     8581 ggcgtcaatg ctgccatcca gatcggagaa cagttcagca atcggttcaa tgcgctcata
     8641 gtgctggcgc gtcggcgcat acagtgcttt cgctttttcg atatcgccag ctttaatcgc
     8701 gtcggtaaag gctttggtgt cggtcaccag ctgcgtggtt tccgccatga catacgcttt
     8761 atatgcagta attgcaccac caagacttaa cagcgcatca ctttgcgccg catccgccgt
     8821 tgcctcacct ttgacgatca acttcccttt cgggttagtc agcagaccgc aggtcatatc
     8881 gtattcgcca ggctgtaaat tcgccgtcat tttctggcta aaaccagggg cgatattttc
     8941 ccgctcttcc accaccatca cgcctttgag gatctcccac tccagcgcct tctggctatg
     9001 gttctgaata atgaactgtg ttttcccggc gttaaccgta atggtcatcg gttcgcactg
     9061 cttatccgtc acggtcactt tgacctgcgg cacatcagcg gcgttagcca taaaagcaga
     9121 agaaaacagc gcagccacgc tcaactgcaa tgcgttacgg cagaagttaa tggtcatgac
     9181 gagactatcc ctttaaagta tgttgtaact aagcaagagt ttgcgtcgta tttgttacgc
     9241 ggagcgagac gctgtcgccc ctgcgcgtgg tggcagagca aatgccacca gcgccgggat
     9301 gagataaata aaccagacgg cgacttcgct gacgctcggc gcttcctgat agccaaaaat
     9361 cccttccatc agcgtgccaa acagcgagtg agttgagagc accgcactca tatcgaaggc
     9421 gatttcctga aagtggttcc acaatccggc ttcatgaaat gcgcgaatgg caccagctgc
     9481 cagccctgcg gcgacgaaga gaataaacag gctggtccat ttaaaaaatg caccaagatt
     9541 gaggcgaata ccgccccagt agagcagaaa gccgagcacc acggctgtag caagaccgag
     9601 cattgcaccc agcggcggcc agatcccgac atcttgttga aatgccgcca gcaggaaaaa
     9661 gaccgactcc agcccttccc ttgcaacggc aaaaaagacc atcatcacca gcgcccagcc
     9721 atgatgattt ccacgctaca atgcgctatc gactgcctgt tccagttgca cttacgttgc
     9781 gcgacacttt gcgcatccag aaaaccatcc aggtaaggat caccacggcg atcaccgcca
     9841 cgataccttc aaacagttcc tgttcttttt gcggaaattc gccggtggtt tcgttaatga
     9901 agatccccag gcccaggcac aacgcagcgg caagcaacac gccaatccac atcacaccaa
     9961 tccatcggcc tcgctgggta cgcttaagat agctggcaat caaactgaca atcagcgcgg
    10021 cttcaagtcc ttcgcgcaac ataatgagaa acggaacaaa catgccaccc acccttaaac
    10081 gtttttctga gtcaatctat gcaaagaaaa gtaaatcgca gtgatagcga ttatcattac
    10141 ggtaaagaaa aatacaagcg gaatgtttat aaaatgatgt tggaatgtaa acaaagagag
    10201 gtaatgccag gggatataaa cgattattcc agggtagaaa gatgggattg tttgcaaaga
    10261 accatgtgtt cttgccggat gccgcgtaaa cgccatatcc agccgacaaa actgtccaga
    10321 ctcaatgcat cagagcttcc atgtaaaact gataagcggg tgtatcaagc aatttcgtcc
    10381 atccgcgatt atcaaatcta actttttgcc cggaacataa taacagctcg ggtgttggtg
    10441 gcgtcaaaat cgctgaagcg ttccccgaag gccgggacag cccacatgga tgtgggctga
    10501 gggcgcgtct tacaggacgt tacctcgcgc ccgacccggt agccgaaggg gataagctga
    10561 aggtaccgcg cagcggcgat tttgttcgcc agagcccggg ggtgcacagg gggcggcggc
    10621 gattggccgc cccctgcgcg ctccctgcac cattcaattt atatggcgag gcacattaag
    10681 ggagcgcaac ccgctgctga agccaaacaa aaccgccgca ggctaagtcc cttagctttc
    10741 ctgcaaccgt gacggcggag ccgaatgata gtgcgcatcg gcctcggcaa agcgtttttg
    10801 catcgccgct gacggcgctt tacccagcaa actaaacact acaatcccga tactgccgaa
    10861 gatgaagccc ggaataattt cgtacagtcc cagccagccg aactgcttcc agacgataac
    10921 cgtcagcgca ccgatgatca tccccgccag tgcaccgtta cgcgtcatgc gtgaccacat
    10981 caccgagaac agcaccaccg gaccaaacgc cgcgccaaag cctgcccacg cgtagctcac
    11041 taagcccagc acgcggtttt ccgggtttgc cgccagcgca atcgccacca gcgccaccac
    11101 cagcaccatc acacgcccta cccacaccag ctctttctgg ctggcatgtt tacgcagaaa
    11161 cgctttgtat aaatcttcgg taatcgcact ggagcacacc agcagttggc aacttaaggt
    11221 tgacattacc gccgccagaa ttgccgacag cagaatcccg gcaatccacg ggttaaacag
    11281 aatttgcgcc agttcgataa acacgcgctc ggcgttctgg tttaccgcgc ctgctaccgc
    11341 cgggtgctca ttaaagtaag caatcccaaa gaagccgaca gccaccgccc ctgccaggca
    11401 gaggatcatc caggtcatac tgatacgacg cgcatggaca atgctgtggt gagaatccgc
    11461 cgccataaaa cgcgccagaa tgtgcggctg cccgaagtaa cccagccccc aacccatcag
    11521 tgagataatg gcaacaaagt tcagaccttt gagcatatca acgttttcga tgctcttttg
    11581 tttgatcact tccagcgagt caccaaagcc accgacactg ataatgacga taaccggtgt
    11641 caggatcagg gcaaaaatca tcaggctggc ctgtacagtg tcagtccagc tcaccgcgag
    11701 gaaaccgcca ataaaggtgt aaaggatcgt cgccgcagct cccgcccaca gagccgtttc
    11761 gtagctcatg ccaaaggtac tttcaaacag acgcgcgcct gccacaatgc ccgaagcgca
    11821 ataaatggtg aagaacagca aaataaccag cgcggagata atgcgcaaaa tgcggctttt
    11881 atcttcaaaa cgcccggtga aataatccgg caatgttaag gcgttattat tgtattcggt
    11941 atgcacacgc aaccgcccgg ccaccagctt ccagttgatc cacgcgccta atgtcaggcc
    12001 aatggcgatc cagctttcgg aaatcccgga aagaaaaacc gcgccaggca accccattaa
    12061 cagccagccg ctcatatccg acgcacccgc cgataatgcc gtcacaaatg gcccaagact
    12121 acgaccgccc agaatatagt cgtcaaagtt tttcgttgat cgccaggcga taaacccaat
    12181 caatatcatg ccaaagatat agacacaaaa tgtcaccaac atcggtgtgc taatagccat
    12241 ctaaagtctc caaaaaatta ttatcggcaa tgtcgaaact tgccgttata tctgcaaccg
    12301 gaacggggta acagagttta ttttttacca gggcgaccgt atcctgccgg aagcgctggt
    12361 tattcacaat cgatttaaca caccatttac attaaatttt agtgctcagc gacactattt
    12421 ttcatcaggt tgcactctct cacatttttt gcggttgcac ctttcaaaaa tgttaactgc
    12481 cgcagagaaa aagtctgagt tattttttta atccctgtca tatcaatttc ttttattaac
    12541 atttcattca tttttaagct tgctacgcag gtcacattta acatggttgc acaaagttgc
    12601 aacatcatgg atatttcacg ataacgttaa gttgcacctt tcagaacaac aggagtaatg
    12661 gcatgggaac caccaccatg ggggttaagc tggacgacgc gacgcgtgag cgtattaagt
    12721 ctgccgcgac acgtatcgat cgcacgccac actggttaat taagcaggcg attttttctt
    12781 atctcgaaca actggaaaac agcgatactc tgccagagct acctgcgctg ctttctggtg
    12841 ctgccaatga gagcgatgaa gcaccgactc cggcagagga accacaccag ccattcctcg
    12901 actttgccga gcaaattttg ccccagtcgg tttcccgcgc cgcgatcacc gcggcctatc
    12961 gtcgcccgga aaccgaagcg gtttctatgc tgctggaaca agcccgcctg ccgcagccag
    13021 ttgctgaaca ggcgcataaa ctggcatatc aactggccga taaactgcgt aatcaaaaaa
    13081 atgccagtgg tcgcgcaggt atggtccaag ggttattgca ggagttttcg ctgtcatcgc
    13141 aggaaggcgt ggcgctgatg tgtctggcgg aagcgttgtt gcgtattccc gacaaagcca
    13201 cccgcgacgc gttaattcgc gacaaaatca gcaactgtaa ctggcagtca cacattggtc
    13261 gtagcccgtc actgtttgtt aatgccgcta cctgggggct gttgtttact ggcaaactgg
    13321 tttccaccca taacgaagcc agcctctccc gctcgctgaa ccgcattatc ggtaaaagcg
    13381 gtgaaccgct gatccgcaaa ggtgtggata tggcgatgcg cctgatgggc gagcagttcg
    13441 tcacgggtga aaccatcgcg gaagcgttag ccaatgcccg caagctggaa gagaaaggtt
    13501 tccgttactc ttacgatatg ctgggcgaag ctgcactgac tgccgcagac gcacaggcgt
    13561 atatggtttc ctatcagcag gcgattcacg ccattggtaa agcgtctaac ggtcgtggca
    13621 tctatgaagg tccgggcatt tcaatcaagc tgtcggcgct gcatccgcgc tacagccgcg
    13681 cccagtatga ccgggtaatg gaagagcttt acccgcgtct gaaatcactc accctgctgg
    13741 cgcgtcagta tgatattggt attaacatcg atgccgaaga ggccgatcgt ctggagatct
    13801 ccctcgatct gctggaaaat ctctgtttcg agccggaact ggcaggctgg aacggcatcg
    13861 gttttgttat tcaggcttat caaaaacgct gcccgttggt gatcgattac ctggttgatc
    13921 tcgccacccg cagccgtcgc cgtctgatga ttcgcctggt gaaaggcgcg tactgggata
    13981 gtgaaatcaa gcgtgcgcag atggacggcc ttgaaggcta tccggtttat acccgcaagg
    14041 tgtataccga cgtttcttat ctcgcctgtg cgaaaaagct gctggcggtg ccgaatctaa
    14101 tctacccgca gttcgcgacg cacaacgccc atacgctggc agcgatttat caactggcag
    14161 gtcagaacta ctatccgggt cagtacgagt tccagtgcct gcatggtatg ggcgagccac
    14221 tgtacgagca ggtcacaggg aaagttgccg acggtaaact taaccgaccg tgtcgtattt
    14281 atgccccggt tggcacatat gaaacgctgc tggcgtatct ggtgcgtcgc ctgctggaaa
    14341 acggtgctaa cacctcgttt gttaaccgta ttgccgacac ctctttgcca ctggatgaac
    14401 tggtcgccga tccggtcact gctgtagaaa aactggcgca acaggaaggg caaactggat
    14461 taccgcatcc gaaaattccc ctgccgcgcg atctttacgg tcacgggcgc gacaactcgg
    14521 cagggctgga tctcgctaac gaacaccgtc tggcctcgct ctcctctgcc ctgctcaata
    14581 gtgcactgca aaaatggcag gccttgccaa tgctggaaca accggtagcg gcaggtgaga
    14641 tgtcgcccgt tattaaccct gcggaaccga aagatattgt gggctttgtg cgtgaagcca
    14701 cgccgcgtga agtagaacag gcgctggaaa gtgcggttaa taacgcgcca atctggtttg
    14761 ccacgcctcc ggctgaacgc gcggcgattt tgcatcgcgc tgccgtgcta atggaaagcc
    14821 agatgcagca actgattggt attctggtgc gtgaagccgg aaaaaccttc agtaacgcta
    14881 ttgccgaagt gcgcgaagcg gtcgattttc tccactacta cgccggacag gtgcgggatg
    14941 atttcgctaa cgaaacccac cgtccattag ggcctgtggt gtgtatcagt ccgtggaact
    15001 tcccgctggc tattttcacc gggcagatcg ctgccgcact ggcggcaggt aacagcgtgc
    15061 tggcaaaacc ggcagaacaa acgccgctga ttgccgcgca agggatcgcc attttgctgg
    15121 aagcgggtgt accccctggc gtggtgcaat tgctgccagg tcagggggaa accgtgggcg
    15181 cgcaactgac gggtgatgat cgcgtgcgcg gggtgatgtt taccggttca accgaagtcg
    15241 ctacgttact gcagtgcaat atcgccagcc gcctggacgc tcagggtcgc cctattccgc
    15301 tcatcgctga aaccggcggc atgaacgcga tgattgtcga ttcttcagca ctgaccgaac
    15361 aagtcgtcgt ggatgtgctg gcctcggcgt tcgacagtgc gggtcagcgt tgttcggcac
    15421 tgcgcgtgct gtgcctgcaa gatgagattg ccgaccacac gttgaaaatg ctgcgcggtg
    15481 caatggccga atgccggatg ggtaatccgg gtcgcctgac caccgatatc ggtccggtga
    15541 ttgatagcga agcgaaagcc aatattgagc gccatattca gaccatgcgt agcaaaggcc
    15601 gtccggtgtt ccaggcggtg cgggaaaaca gcgaagatgc ccgtgaatgg caaagcggca
    15661 cctttgtcgc cccgacgctg atcgaactgg atgactttgc cgaattgcac aaagaggtct
    15721 ttggtccggt gctgcatgtg gtgcgttaca accgtaacca gctaccagag ctgatcgagc
    15781 agattaacgc ttccggctat ggtctgacgc ttggtgtcca tacgcgtatt gatgaaacca
    15841 tcgcccaggt cactggctcg gcccatgttg gtaacctgta tgttaaccgt aatatggtgg
    15901 gcgcagtggt tggtgtgcag ccgttcggcg gcgaagggtt gtccggtacc gggccgaaag
    15961 caggcggtcc gctctatctc taccgtctgc tggcgaatcg cccggaaagt gcgctggcag
    16021 tgacgctcgc gcgtcaggat gcagagtatc cggtcgatgc acagttgaaa gccgcattga
    16081 ctcagccgct aaatgcactg cgggaatggg cagcaaatcg tccagaattg caggcgttat
    16141 gtacgcaata tggcgagctg gcgcaggcag gaatacaacg attgctgccg gggccgacgg
    16201 gtgaacgcaa cacctggacg ctgctgccgc gtgagcgcgt gttgtgtatt gccgatgatg
    16261 agcaggatgc gctgactcag ctcgccgccg tgctggcggt gggcagccag gtactgtggc
    16321 cggatgacgc gctgcatcgt cagctagtaa aggcattgcc atcggcagtc agcgaacgta
    16381 ttcaactggc gaaagcggaa aatataaccg ctcaaccctt tgatgcggtg atcttccacg
    16441 gtgattcgga tcagcttcgc gcattgtgtg aagccgttgc tgcgcgggat ggagcaattg
    16501 tttcggtgca gggttttgcc cgtggcgaaa gcaatatcct tctggaacgg ctgtatatcg
    16561 aacgttcgct gagtgtgaat accgctgccg ctggcggtaa cgccagttta atgacgatcg
    16621 gttaaacagg tagccggagg atgttacacc ctcctccggc atctttaacg tggtcgaatc
    16681 ccctcaataa taatccgctg cacgttttca accgtttgat tgaaaaatac ctcatcgcgc
    16741 aacgttgcgc ctgtcaccgc ctccacctga ggggcgaaat cggcgtaatg ttgagtggaa
    16801 gcccaaataa taaaaatcaa atgctgcgga tcaatcggcg cgagtttgcc gcttttgacc
    16861 caaccggcaa tcagcgccga tttctcatca attaatgcct tcaaatcgcc cgtcagttca
    16921 tccattaaca gcggcgcgcc tgccagcatc tccatacaga acaggcgcga agcctgcgga
    16981 taatcgcgtg agacttccag cttcagacgg atgtactctt tgatcgccgc cagcggggcg
    17041 aaatcttcac gaaacgcttt taacggtgcc agccagatat cgagaatctg ccgcagcacg
    17101 gcaatataca gcgcctcttt tgacggaaag taatacagca gattggtttt tgaaacaccc
    17161 gccaactctg cgatctgctc cagccttgtg ccgtgaaaac cgaattgtga aaaagtgtcc
    17221 agtgctgcgc taagaatcgc ttttttcttc gcgcttactg cgcgcgaacg tttacccgtt
    17281 gttttcactg cgccttgcgt catgcgctct cccctcttgt agattggcaa caggatagca
    17341 aaacccccgc ccggcctcga ccttctgcac tctcatcgcg cacggatgca tgttttatgt
    17401 gcaactgttt tgaccgttta gtccactttt taccagatat tttagataac acaatgctaa
    17461 cacattaata acattcactt tttaaaaact ggcatccgct ttgcaaacaa gccaatacgc
    17521 ggctgaaaag gatatgcagg atgcagcgcc ccgtctgact ttcactttac gagacgaaga
    17581 gaggttgatg atgaaaattg gcgtattcgt acctattggc aacaacggct ggctcatttc
    17641 gacccacgcg ccgcagtaca tgccgacctt tgaactgaat aaagccattg tgcaaaaagc
    17701 ggagcattac catttcgatt tcgccctg
//
LOCUS       DAJVUJ010000083        17493 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.083, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000083 DAJVUJ010000000
VERSION     DAJVUJ010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17493
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.083"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..734
                     /locus_tag="OQQ48_002479"
     CDS             <1..734
                     /locus_tag="OQQ48_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HCS1953211.1"
                     /translation="KPAKLKFHQYARATLLSNRGRDHNCDGRRTVEIHKLDLSDWQAF
                     NKLATKCNAYDGVTMNGDNSFGWNHEATLDNIHAQKYNKAYAGARLTAELKYLLQDVE
                     SFEPNSKYTIHEVVLGPGYGTPDYTGQTIGYVVTLPAQMPNCWSSELPTIDLYIDQLH
                     TVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSNSAAIYSGLGFERVPQDRDIRSD
                     RMHLTPANHPDLWTQENGEWIYLRN"
     gene            complement(805..1254)
                     /gene="rplI"
                     /locus_tag="OQQ48_002480"
     CDS             complement(805..1254)
                     /gene="rplI"
                     /locus_tag="OQQ48_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCS1953212.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(1296..1523)
                     /gene="rpsR"
                     /locus_tag="OQQ48_002481"
     CDS             complement(1296..1523)
                     /gene="rpsR"
                     /locus_tag="OQQ48_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCS1953213.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            complement(1528..1842)
                     /gene="priB"
                     /locus_tag="OQQ48_002482"
     CDS             complement(1528..1842)
                     /gene="priB"
                     /locus_tag="OQQ48_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HCS1953214.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            complement(1849..2244)
                     /gene="rpsF"
                     /locus_tag="OQQ48_002483"
     CDS             complement(1849..2244)
                     /gene="rpsF"
                     /locus_tag="OQQ48_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCS1953215.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            2571..2846
                     /locus_tag="OQQ48_002484"
     CDS             2571..2846
                     /locus_tag="OQQ48_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCS1953216.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            complement(2921..3472)
                     /locus_tag="OQQ48_002485"
     CDS             complement(2921..3472)
                     /locus_tag="OQQ48_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1440 domain-containing protein"
                     /protein_id="HCS1953217.1"
                     /translation="MAINGLFVRTEPQRRRYGVALLVGVLSGIISAFVKWGAEVPLPP
                     RTFSGGRDEFNPPYIFLRDYLGIDPTQTVYTFSEHIINPVMVTHIIFSLVFAIGYCIV
                     AEIFPKVKLWQGILAGLIVTVAVHGIFCPALNLTPPLTQLPFDEYASEILGHIFWFWI
                     IEIMRRDLRNRITHEPDPEVAIR"
     gene            complement(3569..4255)
                     /gene="ulaF"
                     /locus_tag="OQQ48_002486"
     CDS             complement(3569..4255)
                     /gene="ulaF"
                     /locus_tag="OQQ48_002486"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HCS1953218.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGKYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGDAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(4255..5109)
                     /gene="ulaE"
                     /locus_tag="OQQ48_002487"
     CDS             complement(4255..5109)
                     /gene="ulaE"
                     /locus_tag="OQQ48_002487"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HCS1953219.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKARDWVKARMA
                     KAGMVEAA"
     gene            complement(5119..5769)
                     /gene="ulaD"
                     /locus_tag="OQQ48_002488"
     CDS             complement(5119..5769)
                     /gene="ulaD"
                     /locus_tag="OQQ48_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HCS1953220.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            complement(5783..6247)
                     /gene="ulaC"
                     /locus_tag="OQQ48_002489"
     CDS             complement(5783..6247)
                     /gene="ulaC"
                     /locus_tag="OQQ48_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HCS1953221.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            complement(6257..6562)
                     /gene="ulaB"
                     /locus_tag="OQQ48_002490"
     CDS             complement(6257..6562)
                     /gene="ulaB"
                     /locus_tag="OQQ48_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HCS1953222.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            complement(6578..8026)
                     /gene="ulaA"
                     /locus_tag="OQQ48_002491"
     CDS             complement(6578..8026)
                     /gene="ulaA"
                     /locus_tag="OQQ48_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCS1953223.1"
                     /translation="MSAQHSGCAEYPVTSSSGDRLYNIFTVFFNQVMTNAPLLLGIVT
                     CLGYILLRKSVSVIIKGTIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISD
                     TYASMMATIDRMGDAYSWVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGL
                     IAVTLFIFGYSMWTTIICTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASW
                     IAYKVAPFLGKKEESVEDLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAM
                     AGKVNWTVYILQTGFSFAVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAA
                     IYSFAPNAVVWGFMWGTIGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFG
                     GWRAALKICLVMGMIEIFGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMA
                     VIIVIALAYMFFAGRALRAEEDAEKQLAEQSA"
     gene            8330..9394
                     /gene="ulaG"
                     /locus_tag="OQQ48_002492"
     CDS             8330..9394
                     /gene="ulaG"
                     /locus_tag="OQQ48_002492"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HCS1953224.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTPPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQAAPQEIRVQWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            9502..10257
                     /gene="ulaR"
                     /locus_tag="OQQ48_002493"
     CDS             9502..10257
                     /gene="ulaR"
                     /locus_tag="OQQ48_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HCS1953225.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(10254..11003)
                     /gene="yjfP"
                     /locus_tag="OQQ48_002494"
     CDS             complement(10254..11003)
                     /gene="yjfP"
                     /locus_tag="OQQ48_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCS1953226.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSSDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIIAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            11185..11514
                     /gene="bsmA"
                     /locus_tag="OQQ48_002495"
     CDS             11185..11514
                     /gene="bsmA"
                     /locus_tag="OQQ48_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HCS1953227.1"
                     /translation="MVSRKRNSVIYRFACLLLVLMLSACSALQGTPQPAPPVTNHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            11663..11938
                     /gene="yjfN"
                     /locus_tag="OQQ48_002496"
     CDS             11663..11938
                     /gene="yjfN"
                     /locus_tag="OQQ48_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HCS1953228.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(12055..13680)
                     /locus_tag="OQQ48_002497"
     CDS             complement(12055..13680)
                     /locus_tag="OQQ48_002497"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HCS1953229.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSLLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMCHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(13764..14822)
                     /locus_tag="OQQ48_002498"
     CDS             complement(13764..14822)
                     /locus_tag="OQQ48_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCS1953230.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNAIQERLISRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQ
                     QAGQESRFIYIEDLGLGVGGVLTDLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQW
                     VEPLWKSILSNKGLMPLLWRFFPGHPNLLASWFEGEKPQIAAGESYVRKPIYSREGGN
                     VTIFDGQNNVVDHADGDYADEPMIYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIRED
                     NTLITKDTSRFVPHYIAG"
     gene            complement(14825..15463)
                     /locus_tag="OQQ48_002499"
     CDS             complement(14825..15463)
                     /locus_tag="OQQ48_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HCS1953231.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCGDSGDVDNDGDGTFYSTVQDCIDDGNNADICARGWNNTKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWLNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            complement(15473..15871)
                     /locus_tag="OQQ48_002500"
     CDS             complement(15473..15871)
                     /locus_tag="OQQ48_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HCS1953232.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAVSLAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            complement(15889..16548)
                     /locus_tag="OQQ48_002501"
     CDS             complement(15889..16548)
                     /locus_tag="OQQ48_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HCS1953233.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVAAVSRIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKTMGAMTLNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            complement(16599..17297)
                     /locus_tag="OQQ48_002502"
     CDS             complement(16599..17297)
                     /locus_tag="OQQ48_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HCS1953234.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSR
                     AQDVLARLQRQQGE"
     gene            complement(17370..>17493)
                     /locus_tag="OQQ48_002503"
     CDS             complement(17370..>17493)
                     /locus_tag="OQQ48_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HCS1953235.1"
                     /translation="QKMMPLSSVGISSVQQEEYYIVFGALSLKSSLEDILLEIT"
BASE COUNT         4462 a         4672 c         4186 g         4173 t
ORIGIN      
        1 acaaaccagc aaaattaaaa tttcatcaat acgcacgcgc tacattactg tcaaatcgtg
       61 gaagagatca taattgcgat ggcagacgta ctgttgagat ccacaaacta gatctctctg
      121 actggcaagc attcaataag ttagcaacga agtgtaatgc ttacgatgga gtaacaatga
      181 atggtgataa ttcttttgga tggaaccatg aagctacatt agataatatt cacgcccaga
      241 aatacaataa agcttacgcg ggtgcgaggt taaccgcaga actgaaatat ttattgcaag
      301 atgtcgaatc cttcgaaccc aacagtaaat acacaataca cgaagtggtt ttgggacctg
      361 gttatggtac tccagactat accggccaaa cgataggata cgttgtaacc cttcctgccc
      421 aaatgcctaa ttgctggtca tctgaactac caacaatcga tctttatatc gaccaacttc
      481 acactgttac aggcgtttct aatgccctcg gattcatcat tgctgcattg ctaaacgcgt
      541 attctgattt accccatgat ttgaaaattg gtctgcgttc cttatcaaat tcagcagcaa
      601 tctactcggg tttaggtttc gagcgagtgc cgcaggaccg agacataagg agtgatcgta
      661 tgcatctaac gccagcaaac catcctgact tatggacaca agagaatggt gaatggatct
      721 acttgcgaaa ctaatacctg acgaaaaata gaaagcaaaa cgccgaccaa tggtcggcgt
      781 ttttacgtct cgttgaataa cgaattattc agctactacg tttacgatca ctttcgcgaa
      841 tacttcgctg tgaacctgga agctcacttc gtgttcgcca gtggtacgca gaacgccgtt
      901 cggcagacga acttcgctct tagccacttc aacgccagct gcagttacag cgtcagcgat
      961 gtcgcgagta ccgatggaac cgaacagttt accttcgtcg ccagctttag acgcgatggt
     1021 aacagtttcc agtgcattga ttttctcagc gcgagcatta gcagctgcca gaacttcagc
     1081 cagtttagct tccagttcag cgcgacgtgc ttcgaagaat tcaatgtttt tcttggtagc
     1141 tggaacagct ttaccctgcg gtaccaggaa gttacgagca tagcccgctt taacgtttac
     1201 ctgatcacct aggctaccca ggtttgctac tttatcaagc agaataactt gcattacctt
     1261 atcctctcaa agtcgtatta atggaccgtg accgattact gatggcgatc agtgtacggc
     1321 agcagggaca ggtagcgagc gcgtttgata gcgcgagcca gctgacgctg gtattttgca
     1381 cgggtaccgg tgatacggct tgggacaatc ttaccgcttt cggtgatgta gtttttcagc
     1441 gtagcgatat ctttatagtc gatctcttga acgccttccg cggtgaaacg gcagaacttg
     1501 cgacgacgga aataacgtgc catatggcta gtctccagaa tctatcaatt caatctgctc
     1561 ggcatgcaaa accattttgc tcagtccgtt ctttgccttg tggcatgaaa tgaacccctg
     1621 aacggttatg cgactgccga ccgttatact gtgagtaatg gcttggtttt cgtgtccgct
     1681 aacaataacg ggcatttggc accacgcctg ccggtgaaaa ccggcttcct cctgcacaga
     1741 acgatgctca agcacgaact ggcagtgagg aattcctgat ggactgacct ttcgaagggg
     1801 agtcctgcac acggtgccgg acaacaccag acggttggtc atcagaaatt actcttcaga
     1861 atccccagct tcagcatcat cagcggtttc gtttgcgaaa tcatcgcgac gctcacggcg
     1921 ctcgtctttc gctttaacca tcggagatgc ttcggtaaca gcgtgcttgg tacgcataac
     1981 catgctgcgg ataacggcat cgttgaagcg gaaggtagtt tccagctcat cgatcacttc
     2041 ctgcggagct tcaacgttca tcagaacgta gtgtgctttg tgcagtttgt tgatcgggta
     2101 agccagctga cggcggcccc agtcttccag acggtggatc ttgccttctg caccagtgat
     2161 ggcagcagtg tagcgctcga tcatgcccgg aacctgttcg ctctggtcag gatggaccat
     2221 aaaaacgatt tcgtaatgac gcatcgaatt gctccttacg gattattcag cctcctgtct
     2281 gggtcagccg aatcccgggg aggcaaggaa cgtgttaaag gtcggctgaa aaatgacgcg
     2341 ttatagtact tgtatgcccc actaaactca aggccagccg acaaataaat ctcatctttc
     2401 gctcaaaaga actaactcgc tgatttgaag ttcatcacat tccgtcacag tgttttttcg
     2461 aacaactgca tcagaaatgg tcaccatcaa cttctttatc attgattaaa attaaatcag
     2521 aagatcatca cagaggagag cacacaaagt cagccgtcga ggaggtatct atgttcagtc
     2581 gtgttttagc ccttctggct gtgcttttgc taagtgcaaa tacatgggca gccattgaaa
     2641 ttaataacca ccaggccaga aatatggacg acgtacagag cttaggcgtg atttatatca
     2701 atcataattt cgccactgaa agcgaagcac gtcaggcatt aaatgaagag acagatgcgc
     2761 agggcgcaac gtactaccac gtaattctga tgcgggaacc ggggagtaac ggcaatatgc
     2821 acgccagcgc ggatatttat cgctagcacc aggtataacc aacgaaacat tgccatagtt
     2881 tgctttgccc ccttcgcagg gggctttttt atacggttat tcaccggatt gcgacttccg
     2941 gatctggctc atgagtaatg cgattacgta agtcgcggcg cataatttct attatccaga
     3001 accagaaaat atgccccagg atttcagagg catattcgtc aaaaggcaat tgagtgagcg
     3061 gcggcgttaa attcagcgcc gggcaaaata taccgtggac tgccactgtc acaataagcc
     3121 ctgctaatat cccctgccat aatttcactt tcgggaaaat ttccgcgaca atacaatacc
     3181 ctatcgcaaa aaccagtgag aagatgatat gcgtcaccat caccgggttg ataatatgtt
     3241 ccgagaatgt atacacagtt tgggttgggt cgatacctaa ataatcccgt agaaagatat
     3301 acggcgggtt aaattcatcg cgaccaccgg aaaatgtgcg cggtggcaag gggacttccg
     3361 ctccccattt aacaaatgct gaaataattc ctgaaagtac acccacaagt aacgcaacgc
     3421 catagcggcg acgttgcggc tccgttctga caaacaatcc attgatagcc ataaaaacac
     3481 ctgttgttta tacgattttt taactgaagc ggccccggaa tcagaccaat tacatcccgc
     3541 aggaggtaat aattccgggg cgcactgctt acttctgccc gtaataggcg ttaggaccgt
     3601 gcttacgcat gaagtgttta ttcatgagga agctgtcgat gtgattcagt tgtgggttaa
     3661 tactgcgcgc aatccacgcc attttcgcca cttcttccat caccaccgcg ttatgcaccg
     3721 cgtcgtgggc atctttcccc caggcgaacg gcccgtgctg atacaccaca attcccggcg
     3781 tatgcagcgg ctcggcgtca cccagcgttt cgataatcac tttgccggtg ttcagttcat
     3841 actcgccctg cacctcttct tcgcttaatc cacgcgtaca cgggatgtcg ccaaagaagt
     3901 agtcggcgtg cgtggtgcct aacgccggga tcgccagccc cgcctgcgcc catgcggtgg
     3961 catgagtgga atgggtatgg acaatgccgc cgagcgacgg gtaacgacgg tagagttcaa
     4021 gatgcgtcgc ggtgtcagaa gacgggcgat acttcccttc taccaccttg ccgctcatat
     4081 cgaccaccac catatcgtct gctttcatgg tttcgtaggc aacgccgctg ggcttgatca
     4141 ccaccagccc gcgttcgcgg tcgatagcgc tgacgttgcc ccaggtaaag gtcaccagcc
     4201 catagcgcgg cagatccatg ttggcttcaa acacctgctg ttttagcttt tgcattatgc
     4261 cgcctccacc atgcctgctt tggccatgcg cgctttcacc caatcacgcg ctttcaccac
     4321 ttctgccgcc gggtcttccg ccgtttcgct ccacatctca atcaggtacg gcccgcaata
     4381 gccactctgt ttgagcgttt cgaagcaacg ttcgaaatcc accacacctt cgccaaatgg
     4441 cacgtttttg aagacgcccg gtttggtgtc tttcacatgc accgcgacga tatgcccgat
     4501 tccggcctgc aactccatct gcacatcgtt gtcccacgcc gacaggttgc cgatatctgg
     4561 gtagagctgg aaccacgggt tgttgagata gtgcgcgtaa cccagcgcct tgctgatgga
     4621 gttcatcaac ggataatcca tgatctccat cgccagcgtc acctgcgcgc ggctcgccat
     4681 ctcaacgctc tctttcaggc cgtcacggaa acgacgacgc gtttcgttat tggcttcctg
     4741 atagtaaacg tcatagcccg ccagctggat cacgcgaatg ccaacgtcct gggcgaactg
     4801 gatggcttta cgcataatct ccagcccctg cgcccgcacc gcgtcatctt cgctgcccaa
     4861 cgggaaacga cgatgggcag aaaggcacat ggacggcaca cgcacgccgg tttcaacaat
     4921 cgcattcacc agcgccagac gctgctcgcg gctccagtcg aggcgcgaca ggcgctcgtc
     4981 agtttcatct accgacatct cgacaaaatc gaagcccaac gtttttgcca gtcgcaggcg
     5041 ttccagccag cactccccgg cggggagcgc tttttcatag atgccaagcg ggatttgttt
     5101 ggacaacata tccgctcctt agccccacag ttcggcgatg gaacgtttga actgacgcgc
     5161 ggcttcaacc ggagaagcgg catcacggat gctgcgaccg gcgataaaca catgaattgg
     5221 aatgcctttg aacagcggca gatcttccag cgccaggcca ccggtgacgg tgactttgaa
     5281 gcccatatcg gaaagacgtt tgatcgcagt gatatcggct tcaccccacg ccacgccagc
     5341 ggcctgcgcg tcacggctgc ggtgataaac cacctgctga atacctgcgt cgcgccactg
     5401 ttgcgcctgt tcccaggtcc agtaaccggt cagttcgatc tgcacgtcgc cgttaaactc
     5461 ttttgctacg tccagcgcac ctttggcggt gttgatatcc gcacaacaaa tcacggtcac
     5521 ccagtcagcg ttggcttcga agcacatacg agaaagaatt ttgcccgcat cggcaatttt
     5581 ggcgtctgcc agcacgattt tgtgcgggta gagcgctttc agatcgcgaa ccgcacgcac
     5641 gccttcgccc acgcacagaa tggtacccac ttcgataatg tcgacttctt cggcaatcag
     5701 gcgagtggtt tcgtaggcgc tatccatagt ctggttgtcc agcgcgactt gcaacatcgg
     5761 taatgacatc ttcaattcct tcttaagctg ccgcgttggt gcggtcaatg agatccagta
     5821 cttcctgctc ggtacggcag gcacgtaaac ggtcgaaatt ttcttcatct tcaaacaggt
     5881 tgacgatctg catgatgccc acttcctggt gcgtgttggc atcgaccgcc gccatggtga
     5941 tgaggatatc caccgggtcg ttatcttcgt ggttgaactc cagcggcttt ttcagcgtca
     6001 ccagcgcaaa accggttttc ttaacgccct cttccgggcg accgtgcggc attgccaggc
     6061 ctggtgcgag cacaaagtac ggaccgtgct gttcaaccgc atccagaatc gcctggtagt
     6121 aacgcggctc gacaacatcc gccgccacca gcaggtcaac gccgattttt actgcgtcct
     6181 gccatgtctc agcttcagcc tgcaggcgga tggatttatt ctccgccagc gaatcacgta
     6241 atttcatggc gcgtccttac ttcacatcct gcgggaaatg cgctttgatc acttccagca
     6301 gtttcgggcc aaagtcggca ggagagagca tgttgcgcac gccaaccacg tatttgttgc
     6361 cggtcacggt gatttcgccc gcaatgtgcg tagaagcgat gatgatatcc gcgccgctca
     6421 actcgctttt gtattcgcca accgcgcagc tatttaccgt gtggtcaatg tttgattggg
     6481 ttaaaaactg gtccactttc atcttcatga tcatggaact gccttgtccg ttgccacaca
     6541 cagccagaat acgtacggtc ataatcaaaa ctccttatta agcagactgt tctgccagtt
     6601 gtttttctgc atcttcttct gcgcgcagtg cgcggccagc gaagaacata taagccagtg
     6661 caattacaat gatgacggcc ataaaggcga taccgatgga gaagaagccc tgcatcatcg
     6721 gcggtgccag aatcgaccag tccgccatgc ccatccaggc gctcataccg gtgagtttca
     6781 ccgcccagac gcagccaaag atttcaatca tccccatcac cagacatatc ttcagcgccg
     6841 cacgccagcc gccgaagtgg ttagcgaaca cgccgatggt ggcgttggag aagaacatcg
     6901 ggataaagcc gggaataatc aggatcgagg aaccgcaggc gaccaggatg ccaaccgcaa
     6961 tcagctgacc gatggtgccc cacataaagc cccaaaccac ggcgttcggc gcgaagctat
     7021 agatagccgc acagtcaatc gccagaaccg caccagggat caggcgctgg gagataccgt
     7081 taaatgcttc agagagttcc gccacaaaca tgcgcacgcc ctgagtgatg atgaagatcg
     7141 ccaccgcgaa ggagaaacca gtttgcagga tgtacaccgt ccagttcact ttgcctgcca
     7201 tcgcctgcac ggtgtcgata ccgaaggaga gtagaatggc accaaagaag atggtcatca
     7261 caatcgccgt ggagacgatg ttgtcgtgga aaatattcag ccagcctggc agtttgaggt
     7321 cttcaacgct ttcctctttt ttgccgagga acggcgcaac tttataggca atccatgatg
     7381 caaactgttg ctggtgaccg atggagaaac cgcagccgtc cgtcacttcc tgagtcggct
     7441 tgtacatcat attggaggta atgccccagt agagcgaaac cagaatcgcc gtgcagataa
     7501 tggttgtcca catggagtag ccgaaaataa acaacgtaac ggcaatcagc cccgcctgct
     7561 ggaacatgat atgaccagtc agcatgatgg tacgaatacc ggtaatgcga cgcagcagca
     7621 cgtaacagat gttcagcgcc agcgctaaca acaccgcgta acctacccag ctataggcat
     7681 cgcccatgcg gtcgatggtt gccatcatcg acgcgtaagt atcggaaatt gcgccgttaa
     7741 tgccgtagac ttcggacatt ttcgccacca ccggtttgaa ggtgctggtg aggatgccgg
     7801 accctgcctg caacaacatg aaaccaatta tggttttaat cgtgccttta ataataacgc
     7861 tgacactttt gcgcagtagg atgtagccca gacaggtcac aatacccagc aacaacgggg
     7921 cattggtcat gacctggtta aaaaacacgg taaagatgtt gtagagacga tctcctgaag
     7981 aagaggtaac cgggtattcc gcacacccgg aatgttgtgc actcactcta attttcaaaa
     8041 gcaatcacaa caagattatt tatgattaaa tgtgacgcga cccgcaaata attccatacg
     8101 gatagagtgg gattcacaca acgaaacaat tatttattta aatttagtta aattcattta
     8161 gttaatttaa ctatacattc cattaaatct ttccacactc aaaatcaaca acaacttccc
     8221 tggcaaaaag tcaaaaaatg gtcattgcct tcctgaaaaa atagtgtcag gattaatcaa
     8281 aaccaatcac atattgattc gaattgaact atcatgaagg taaatggcga tgagtaaagt
     8341 gaaaagtatc acccgagaat cctggatcct gagcactttc ccggagtggg gtagctggct
     8401 gaatgaagaa attgaacaag agcaggtcgc ccctggcaca tttgcgatgt ggtggctagg
     8461 ctgcaccggg atctggttga aatcagaagg tggcaccaac gtttgcgttg atttctggtg
     8521 cggcactggc aaacaaagtc acggtaaccc gttaatgaaa cagggtcacc agatgcagcg
     8581 catggctggc gtgaaaaaac tgcagccaaa cctacgcacc cccccgtttg ttcttgatcc
     8641 gtttgcgatt cgccagatcg acgcggtact ggcgactcac gatcacaacg atcatatcga
     8701 cgttaacgtc gctgctgccg tgatgcagaa ctgtgctgat gacgtaccat tcattggacc
     8761 gaaaacctgc gttgatttgt ggattggctg gggcgtaccg aaagagcgtt gcatcgtggt
     8821 caaaccgggc gatgtggtga aagtgaaaga cattgaaatt catgcgcttg atgctttcga
     8881 ccgtactgca ctgatcaccc tgcctgccga tcaaaaagcg gctggcgtac tgccggacgg
     8941 catggacgat cgcgcggtga actatctgtt caaaacgcct ggcggctccc tgtatcacag
     9001 cggcgactcc cactactcta actattatgc gaagcacggt aacgaacatc agatcgacgt
     9061 ggctttaggt tcatacggcg aaaacccgcg cggtatcacc gacaaaatga ccagcgccga
     9121 tatgctgcgt atgggtgaag ccctgaatgc gaaagtggtt atcccgttcc accacgatat
     9181 ctggtcaaac ttccaggccg ctccgcaaga gatccgcgtg cagtgggaga tgaaaaaaga
     9241 tcgtctgaag tatggcttca agccgtttat ctggcaggtt ggcggcaaat ttacctggcc
     9301 actggataaa gacaacttcg agtaccacta tccgcgcggt ttcgatgatt gcttcactat
     9361 tgaaccggat ctgccgttca aatcattcct gtaatctgtc gtttataccg gatagcgtgg
     9421 agaagttcgc gctatccggc gtagtatttc ctggctattt caaatatcat ctaaaaaaat
     9481 caaattttat cggaatagct catgactgaa gcacaaagac atcaaatcct cctggaaatg
     9541 ctcgcacaat tgggctttgt gaccgttgag aaagtcgttg agcgtctggg aatttcgcct
     9601 gccactgcgc gacgcgatat caataaactt gacgaaagcg gcaaactcaa aaaggtgcgc
     9661 aatggcgcag aagctattac ccaacagcgc ccgcgctgga cgccgatgaa tctgcatcag
     9721 gcgcagaatc acgatgaaaa agtacgtatt gctaaagcgg cctcgcagct ggttaatccg
     9781 ggcgaaagcg tagtcatcaa ctgcggctcc accgcgtttc tgcttgggcg ggaaatgtgt
     9841 ggcaagccag tgcaaatcat cactaattat ctaccgctgg caaattacct gatcgatcaa
     9901 gaacatgaca gcgtgatcat tatgggtgga cagtacaaca aaagtcagtc catcacttta
     9961 agcccgcagg gcagcgaaaa cagtctctat gccgggcact ggatgtttac cagcggaaaa
    10021 gggctgaccg cagaagggtt gtataaaacc gatatgctga cagcaatggc agagcagaag
    10081 atgctgagcg tggtagggaa actggtggta ctggttgata gcagtaagat tggcgaacgc
    10141 gcgggaatgc tttttagccg tgccgatcaa atcgatatgc ttatcaccgg caaaaatgct
    10201 aacccggaaa tcctgcaaca actggaagcg caaggggtca gcattctgcg tgtttaaaga
    10261 tgctggcgga aaaatgtcac ggcagcatct aacgcctcag gcgtaatgcg gtgacgcacg
    10321 cctggctgcc atgaacaggt tagcagttta tcccgtcccg tctcgcttaa ggcctgctgc
    10381 aaacgtagtg attcgtcggc aggcacaacg tcatcgtcga ggccatgcca cagcagtaga
    10441 ggtctgtccc caagttgttc caggtggttt gtcgcttccc actctgccag cggcgcgata
    10501 atgttattga attcattctg ctgtgctgcc gtttcaggta tcagcggtgg aaacagtgaa
    10561 cgggcgagcg atgtaaaata gcccgatccc atcatgctgg cggtacattt caccgtgggg
    10621 tggcgagcgg taattcccag tgccgtcatc gcgcccatcg acgcgccacc gactgccaga
    10681 cggtcatcaa gcagccagtt ttcttcggct attgccgcac gtaaagtagt gaattcctgc
    10741 atactttgta gcaagatttg ccagaattga tttaaccgcc gcgcttcgtc gctactaaaa
    10801 cggctaccgt gatcgggcgc atccggcatg atcacccgca aaccagcctg cgccagcgca
    10861 acggcaaaat agctatacac caggctggat gaagtaaagc cgtgataaaa aattacgcac
    10921 ggtaacgggg tatctttttg cccgacagga taagcatgaa gaacgggaat atctgccagc
    10981 tcgcgtgatt ctatttcaat catctgtctc tcctttttgt ctggtatgac atgaagaaat
    11041 ccttagcaca agtttctgct ggcggtatta gttcataatg ttgagatgtg ggttacgctt
    11101 tcgtcacgtt ttcattcgaa aatcgcgtac caggtaacaa ttcgggaaca ttcccccaaa
    11161 gttaaattaa taaggcacta cactatggtt agcaggaaac gtaatagcgt tatttaccgg
    11221 tttgcctgtt tattattggt gttgatgtta agtgcctgta gcgcactgca aggtacgcca
    11281 cagccagcac cgccagtaac gaaccatccg caagagattc gccgcgacca gacgcaaggg
    11341 ttacagcgaa taggtagcgt aagcacgatg gttcggggtt ctccggatga cgcattagca
    11401 gaaatcagag caaaagctgt cgctgcaaaa gctgattatt acgttgtcgt catggtggac
    11461 gaaaccatcg tgacaggaca gtggtattca caagccattt tgtaccgtaa ataaatcaga
    11521 caacttacat agtcagcttt acattgcttt gcgtccctat gcgtttacct gtgcacaata
    11581 aattaccggc tggaagcctg ggcgtttatt accgcgatgg aatgccctgc aacatgtggg
    11641 gaaacgaaaa atggagctga cgatgaaaca attacttgct tcaccctcgc tgcaattagt
    11701 gacttatcct gcgagcgcca cggcacagtc tgcagaattc gccagtgctg actgcgtgac
    11761 gggcctgaat gaaataggtc agatctcagt tagcaatatt tcaggggatc cgcaggacgt
    11821 ggaacgtatc gtggcgttaa aggccgatga acaaggtgcg tcatggtacc gcattattac
    11881 catgtacgaa gaccagcagc ctgataactg gcgcgtacag gcgatactgt atgcatgagt
    11941 gtcatctaca gcaaatgaac aatgccggat tcggcgcgag cgccttgtcc ggcctacaat
    12001 tttcacatat ttcattgagt taatcgaaac cagcgtcgca tcagtcgtat acgcttacac
    12061 acacactccc cccgtcgccc gcagcaataa atcattctgg atctgctctg acaaccgtac
    12121 gccgccgcgc gtatctaaca tcacctgaca ccacgcctgc gccattggcg gagaagcata
    12181 tttcaacatt tgcgcaccgc agcccagcag gaatagctga tgagtaatct ctcgccccag
    12241 ttcttcagct ggcttacgca gctgctgctg taaacgacga accgcgcgat caaaatagcg
    12301 atcctgccct ttcacttcca caaatgcttc cgacaataag tcgtatacgc ccgcttgctt
    12361 attgagaacg cgcaacacat ccaggcacat aatattgccg gaaccttccc aaatactgtt
    12421 taccggcatc tcccggtaaa gccgcggcag ctcgctctcc tcgcaataac caatgccgcc
    12481 cagcacctcc atcgcttcgg ccacaaacgg catacctctt ttgcagatca caaattttgc
    12541 cgcaggcgta aataaacgcg cccacagggc ttctttggca tcggcacgcc ggtcccacgc
    12601 tcgcgcaaga cgaaacagca acgccgtttg cccttcaagc tgaagtgcca tgcgacttaa
    12661 gacatgacac ataaggggct gttggatcaa tggattacca aaaacatggc gttgatgtgc
    12721 atgataaatc gccagcgaaa atgcacggcg catcatggca tggctaccca gggcgcaatc
    12781 aaaacgcgtc atcccaccca ttttcaggat cagacgaatt ccttcccctt cctgccccaa
    12841 caaccagcca atggcatcct gaaactccac ttcgcaactg gcgttagagc gattacccag
    12901 cttatctttc agccgctcga ggcgaatcgc gttgcgttgc ccatcaggca aaaagcgcgg
    12961 cacaaaaaag caggacaaac cgcccgtagt ctgcgccagc accagatgcg catcgctttg
    13021 cggcaccgag aaaaaccatt tatgccccac cagccgataa gagccatctt ccagacgctc
    13081 tgcacgggtg gtgttgctca tgacatcgga accgccctgc ttttccgtca ttcccatgcc
    13141 aatcaacaaa ccgcgttttt gcccacctgg caataagaga gaatcgtagc gatcgctcag
    13201 cagcggcgtg gtccagtcct gaaacggcgt gggtaacatc tgtaacaaca atggcgtggc
    13261 ggcaaaggtc atcgttattg gacataacga cccagcctca acctgcgcat gtaacataaa
    13321 acgcgccgcg cgcgccacaa atgcgccgga gcgagcgtct tcttcccagg caagattgtg
    13381 cacccgattg gtacataacg cctgcatcag caggtgccag gcggggtgaa aacgcacatc
    13441 gtccagacgg cgtccttgcg catcgtagcg caataattca ggcggattca cattcgccag
    13501 ccgccccagt tcaagagatt cagccgttcc taactgctga ccaatactgg ctagaaaatc
    13561 gctatcccag ccagcacctt cacgcgttac cgcttcacag agcgcgccat cagacaggta
    13621 taagttgctg ttatttaatg gtataggttg attaaaaacg gtgtgagttt gccagtgcac
    13681 tgtgtctccc tccatcaatg gcagtcaccc ttagtatggt cactgccatg gattcctgac
    13741 agaaagagat tgctaaaaca ttcttatcca gcaatgtaat gcggaacgaa acgtgaggtg
    13801 tctttggtga tcagcgtgtt atcttcacgg atccccattc cgcacgcttc atcatcgaca
    13861 atccagctac cgatgagtgt gtagctatcg ccaaatcgcg gcagaggttg aaacgcctgg
    13921 tagatcatcg gttcatcggc gtaatcacca tcagcgtggt caacgacgtt attctgaccg
    13981 tcgaaaatgg tgacgtttcc gccttcgcgt gagtagatcg gtttacgcac atagctttcg
    14041 ccagcggcaa tctgcggttt ttcgccctca aaccaggatg caagaaggtt aggatgacca
    14101 ggaaagaagc gccaaagcag cggcattagc cctttattac tcaagatact tttccataac
    14161 ggctccaccc attgctcgcg acgcttgcgc agcagcggac cgttatcgtc acgcatcatc
    14221 cactccagcg gatacagctt aaatgcacgc tggatgacat tatcatcaag atcggtcagt
    14281 acgccgccga cgcccaaacc gagatcttca atgtagataa accgcgactc ctgccctgcc
    14341 tgctgggcgc agtcctgcaa gtacagcacg gtactcctgt cttcatcggt gtcctgacag
    14401 cagcaaaaat aaaacggttc ccgactgtaa agctcactaa agcgcgaaat caggcgttcc
    14461 tgaatggcgt tgtactccag cagcttcacc ggcgcattgc cacaccaggc aaaatccatc
    14521 cggccataca gcgaaggatc gcgtgcgcgc cagctttcgg cgatcacatc ccagtacaac
    14581 ggcgggatcg ccagttgcgt caggatctct tcgtctttaa ccgcgcgatc caccacctca
    14641 aggcacatct gatgcagttc cgcagtcggt ttttcgatct gctcttcaat ctggcgcaga
    14701 gtaaaacggt aagcccgact ctcgtcccaa tagatttcat tgtcgatgat atgaaagtcg
    14761 aaaccgttgt tagcggcgat ctggtccaga tcccgtcgca caggaacgtt gtgtctcagc
    14821 atgattagcc tccccaatgc ccacgggcgc tggaagaacg accatagccg ccgcgcgaaa
    14881 cggttgacgc ttttttggtg gtaaagccgc ctgaagagta agactctttt ttgccagaac
    14941 cggagcgcca ggagtaatca ccggaagtgt tgagccagac ggggcgggaa gcaaatgagg
    15001 atccgccgct gttatagacg aacggctcat cgcgatcttt acgaataacc cggcttaaca
    15061 aaaatccaga aacgaccggg atccaactct gttcaacatt gtcgtagtag caattttcgt
    15121 acttagactg acaattctgc tgagtcatat tcttcggaac atcggcataa aatgccgctt
    15181 tggtgttgtt ccagccacgc gcgcaaatgt cggcattatt accgtcatca atacaatcct
    15241 gcactgtcga ataaaacgtt ccgtcgccgt cgttatcgac atcgccacta tcgccacaac
    15301 ctttcaatac aaaaaaagcg gcaccgccca ttagcgccaa cgttaagtat ttttgtgcgt
    15361 agcgattgcg acgcggttca aaaggattat tcggtctacc aatgcgactg atcgctccgt
    15421 gaccaatttt actgttattt cttgatttgc gttttctggc catgatcgtc cattaccatg
    15481 tcatacaagc tgcgttaaaa ataccgccag ccagcgcggc ggttcccata aacattcctg
    15541 ctgcggtatt gtgattaata attttttcgc ttaatgcggg catataaagt ctgacaccag
    15601 caaaaacgag taactgaatc accagtgcaa tcccgcccca ggcgaaatag tctggaatac
    15661 tcaccgcatt gattgccgca ctggataagg ggatcacgta acccaacaat gtaccgctga
    15721 atgccagtga caccgcggta ttattgtttt tgattaattg ccattcgttg tgcggtgtaa
    15781 ttttggagta gataaacaga aaaataatca ccatagccac gccaataaaa aaataggcac
    15841 taaaggcaag aagtgaatcc agtatatgca ttgtaaacgt ccttgtaatt aaccaataat
    15901 atgaagtgat gtcagcggga tatcgacacc caacgcgcgc gaaaataacc actctggctc
    15961 gccgagatcg ttaaaagatt cctcaccatt taacagtaaa tattcgtaag tatcttcggt
    16021 tacttgccgc tggtagccca tggtaaaatt atggacctcc catttggcat ggttttgatt
    16081 ttctactttt tccagcatgt agacgggttc gatatttccc ggttcttcgc tgttaaaaaa
    16141 tcgctgccag cgtttttcct gccagtttaa ggtcattgcc cccatcgttt tggcgttgat
    16201 cgcctcgcgc cagtgactct ctttactgat accgtagctc tcttcataga caaagagctt
    16261 gatatcatca atgtcatcaa tatcttcgcc gccggtggta ttgatttgca gaaattcatc
    16321 gcctgaagtg tagtaacgga aaatctggct accgcctccc agatcgatgc ggctgacagc
    16381 ggctaccgta aattcctcac ccggtagcgc aatcagcaat tcatcttcca gcaaacgaaa
    16441 cgctaacgtg tcgagcgtaa agccactatt gagatgaagt cccagcggac cgcgagcgat
    16501 agcaggctta ttatccttgc caaacagacg ctggaaaaaa ccagacatat tttatcctta
    16561 aaatagttgc cagcctttta gcgaggctgg caaaaaaatt actcgccctg ttggcgttgc
    16621 agtcttgcta atacatcctg ggctctactt ttattgctac cgccaattcc ggcttccgcc
    16681 agcttttcgt caaggtcgcg accgtctgcg actttctcca actgtgcggc agcatccagg
    16741 cgagcctgac gttcggcctg acgcgtttgc aggcgtttta aggattctgc cgctgtcgaa
    16801 acgctggaag atgcgccaac ggtagaggtt gttacagcct gttgtgcacg ctgcatggct
    16861 tcagtggctt taacgacttc catttgctgc tcaaactgag cgatacgctg cgctgtcgct
    16921 gtaactgctt tttccacgcc atcacgggag acttccagat tcgacaacac ttgctcttca
    16981 gcggtaatga gattctcaag gcgcgcgatt tcttcagcaa cttcgttaat caacgacgga
    17041 ttaacgttct tgcttaacgc ttccagcgca cgggcttcca gactggcttt gcgctcacgt
    17101 aaatctttca gcttatcgtg acttaatttc acccgcgcca gcagatcaac gcgagatttt
    17161 ccggctttat cgagttcagc tttagcgtca cgaatatgct gttccagcat gcgcacgccc
    17221 tgggtttctt caatggattc ttccgcctgg gagataaacg atttccccag cgtaaataaa
    17281 ctttttaaaa ttcccataaa tacccgtcct tgtaattagt gtgaatattc ttctgtaatt
    17341 tcagccagat ccaatgcgtt atctaccagc taggttatct caagcaggat atcttcaaga
    17401 gaagatttaa gcgataacgc cccgaaaaca atgtaatact cttcctgttg cacgctggag
    17461 attcctaccg atgacaacgg catcatcttc tga
//
LOCUS       DAJVUJ010000084        17485 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.084, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000084 DAJVUJ010000000
VERSION     DAJVUJ010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17485
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.084"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(36..2633)
                     /gene="topA"
                     /locus_tag="OQQ48_002504"
     CDS             complement(36..2633)
                     /gene="topA"
                     /locus_tag="OQQ48_002504"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCS1953236.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            3013..3264
                     /locus_tag="OQQ48_002505"
     CDS             3013..3264
                     /locus_tag="OQQ48_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HCS1953237.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(3300..4349)
                     /gene="sohB"
                     /locus_tag="OQQ48_002506"
     CDS             complement(3300..4349)
                     /gene="sohB"
                     /locus_tag="OQQ48_002506"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HCS1953238.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMGSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKY
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            4569..5327
                     /locus_tag="OQQ48_002507"
     CDS             4569..5327
                     /locus_tag="OQQ48_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HCS1953239.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDICPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            5324..5915
                     /gene="cobO"
                     /locus_tag="OQQ48_002508"
                     /pseudo
     CDS             5324..5915
                     /gene="cobO"
                     /locus_tag="OQQ48_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
     gene            complement(5955..6827)
                     /gene="rluB"
                     /locus_tag="OQQ48_002509"
     CDS             complement(5955..6827)
                     /gene="rluB"
                     /locus_tag="OQQ48_002509"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HCS1953240.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(7038..8933)
                     /locus_tag="OQQ48_002510"
     CDS             complement(7038..8933)
                     /locus_tag="OQQ48_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HCS1953241.1"
                     /translation="MAGKFRCILLLIAGLFVSSLSYAENPEIPSYEEGISLFDVEATL
                     QPDGVLDIKENIHFQARNQQIKHGFYRDLPRLWMQPDGDAALLNYHIVGVTRDGIPEP
                     WHLDWHIGLMSIVVGDKQRFLPQGDYHYQIHYQVKNAFLREGDSDLLIWNVTGNHWPF
                     EIYKTRFSLKFPDIAGNPFSEIDLFTGEEGDTYRNGRILEDGRIESRDPFYREDFTVL
                     YRWPHALLSNAPAPQTTNIFSHLLLPSTSSLLIWFPCLFLVCGWLYLWKRRPQFTPVD
                     VIETDVIPPDYTPGMLRLDAKLVYDDKGFCADIVNLIVKGKIHLEDQYDKNQQILICV
                     NEGATRNNEVLLPAEQLLLEALFRKGDKVVLTGRRNRVLRRAFLRMQKFYLPRKKSLF
                     YRPDTFLQWGGLAILAIILYGNLSPVGWAGMSLVGDMFIMICWIIPFLFCSLELLFAR
                     DDDKPCVNRAIITLFLPLICSGVAFYSLYINVGDVFFYWYMPAGYFSAVCLTGYLTGM
                     GYIFLPKFTQTGQQRYARGEAIVNYLARKEAATHSGRRRKGETRKLDYALLGWAISAN
                     LGREWALRIAPSLSSAIRAPEIARNGVLFSLQTHLSCGANTSLLGRSYSGGGAGGGAG
                     GGGGGGW"
     gene            complement(8961..9581)
                     /locus_tag="OQQ48_002511"
     CDS             complement(8961..9581)
                     /locus_tag="OQQ48_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCS1953242.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(9578..10459)
                     /gene="yciV"
                     /locus_tag="OQQ48_002512"
     CDS             complement(9578..10459)
                     /gene="yciV"
                     /locus_tag="OQQ48_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HCS1953243.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLTTLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            10597..10641
                     /gene="trpL"
                     /locus_tag="OQQ48_002513"
     CDS             10597..10641
                     /gene="trpL"
                     /locus_tag="OQQ48_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HCS1953244.1"
                     /translation="MKAIFVLKAWWRTS"
     misc_feature    10618..10711
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            10733..12295
                     /gene="trpE"
                     /locus_tag="OQQ48_002514"
     CDS             10733..12295
                     /gene="trpE"
                     /locus_tag="OQQ48_002514"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HCS1953245.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLTLLDNALPAGVENEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKNTRIQASLFAPNEEEKQRLTARLNDLRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRTGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGIVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            12295..13890
                     /gene="trpD"
                     /locus_tag="OQQ48_002515"
     CDS             12295..13890
                     /gene="trpD"
                     /locus_tag="OQQ48_002515"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HCS1953246.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQRKLEPTNTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGICFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            13891..15252
                     /gene="trpCF"
                     /locus_tag="OQQ48_002516"
     CDS             13891..15252
                     /gene="trpCF"
                     /locus_tag="OQQ48_002516"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HCS1953247.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     GLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPTHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            15264..16457
                     /gene="trpB"
                     /locus_tag="OQQ48_002517"
     CDS             15264..16457
                     /gene="trpB"
                     /locus_tag="OQQ48_002517"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HCS1953248.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVCEFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRETPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            16457..17263
                     /gene="trpA"
                     /locus_tag="OQQ48_002518"
     CDS             16457..17263
                     /gene="trpA"
                     /locus_tag="OQQ48_002518"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HCS1953249.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLRDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
BASE COUNT         4222 a         4672 c         4346 g         4245 t
ORIGIN      
        1 accctttgtt tataaaaacc tgacagaatt aaaggttatt tttttccttc aacccatttg
       61 ccatcaacat aaaatgctga ccagccagtc gcctttccgt ctttttccga agagacatat
      121 tgctgtttgg ttttacggct aaagcgaacc atggtcttat taccttccgg atcctgctgt
      181 ggcgcatcgg ccagataacg cagtttttcc ggcagacggt cgcggaagcg ataaagctct
      241 tccaccagtg gcgcacgcgt ttcacgcgat ttcgggaaag tgttggcagc caggaaaaca
      301 cccgcagcgc cgtcacgcag cacgaaataa gcatctgatt tttcgcacgg cagctcaggt
      361 aatggcaccg gatcttcttt cggtggtgcc acttcgccgt tacgtaaaat cttacgtgtg
      421 tttttacact cttcgttggt gcaggccatg tatttaccga aacgccccat tttcaggtgc
      481 atttcagagc cacatttttc acactcaacg atcgggccgt cataaccttt aatgcggaat
      541 tcgccctctt cgatctcgta accgtcgcag gttgggttat taccacagac atgcaactta
      601 cgtttcggat cgatgagata gctgtccatc gccgtgccgc atttcgggca acgacgtttt
      661 gcgcgcagcg cgttggtttc agcgtcttcg ccttccagta cgttcagcac ttcgttttcc
      721 ggcaccaggt taatggtggt tttgcaacgc tctttcggcg gcagcgcata accagaacag
      781 ccaaggaata ccccggtgct cgctgtgcga atccccattt tgcgaccaca agtcgggcag
      841 tcaatgctgg tcagaaccat ctggttcggg cgcataccac cctcttccgg atctttttca
      901 gctttatcta actgctgggt gaaatccgag aagaagtgat ccagtacagc tttccactct
      961 gcttcgtgat ttgccacctg gtcgaggctg ttttccatct gtgcggtgaa gtcgtagttc
     1021 attaactcgc ggaaattttc ttcaaggcga tcggtgacga tttcgcccat tttttccgca
     1081 tagaaacgac gattttctac tcgcacgtag ccacgatcct gaatggtcga aatgatcgac
     1141 gcataggtag acggacgacc gataccgcgt ttttccagct ctttaaccag cgatgcttca
     1201 ctgaaacggg ctggcggctt ggtaaagtgc tgggctggtg taagttcaac gagcgtcaga
     1261 gcatcgcctt tattaactgc tggtaagatg cgatcttcat cgcctttacg caacgcaggc
     1321 atcacttttg tccagccatc aaaacgcaaa atacgaccgc gtgctttcag gcggaaatcg
     1381 cccgcaccaa cggtcagcgt cgtggagtca tatttcgctg gggtcatctg gcaggctacg
     1441 aactgacgcc agattaactg gtacagtttc tgcgcatctg cttccatatc cttcagcgat
     1501 tccgccatca cattgacgtc agaaggacga atcgcttcgt gcgcttcctg tgagttttct
     1561 ttgctggcgt actgattcgg actttccggc agatatttct taccaaaatt atcgctgata
     1621 taaccgcgaa ccatatttac cgcgtcctga ctcaggttag tggagtcggt acgcatgtaa
     1681 gtgatatagc ctgcttcata caaacgctgc gccatcatca tggttttttt cacgccaaat
     1741 ccaagacggg tgctggcagc ttgttgcagc gtagaggtaa taaaaggagc gccaggttta
     1801 ctggttgtcg gtttgtcttc acgttccagc acgctgtagc gcgctttttc cagcagactt
     1861 accgcagcct gagtttgttc tttgttgacc ggacggaacg gtttgtcgtt ctgatgagtc
     1921 acctgtaacg ccaacgcttc accagatggc gtggtcgtgc tggcatcgac ttcccagaac
     1981 tcttccggca cgaacgcttt aatttcacgc tcacgctcga ccaccaggcg aaccgccacc
     2041 gactgcacac gaccggcaga cagaccacga gcgatctttt tccatagcag cggcgaaacc
     2101 atatacccca ccacgcggtc cataaagcga cgcgcctgct gggcattaac acgatcaata
     2161 ttcagctcac ccggtttgtt aaatgcctgg cggatcgcgt ttttagtaat ttcgttaaac
     2221 accactcggc tatagcgcgc atcatcaccc ccaatcactt cccgcaggtg ccatgcaatg
     2281 gcttcccctt cgcggtcaag gtcggttgcg agatagatgt ggtcggcttt ttcagccagt
     2341 tgtttcagtt cagagacgac cttctcttta ccaggcaaca cttcatagtg cgcctcccaa
     2401 ttgtgccacg ggtcaacccc catacggttg acgagagcgc cacgttcatc ctttttaggc
     2461 tttttagccg tcttggtgga ggtagagtcg gcactctttt tggcagctga gccactggtc
     2521 ggcaaatcgc ggatgtgacc gacgctggat ttcaccacgt agtcactacc cagatacttg
     2581 ttgatcgttt tggcttttgc cggggactca acgatgacaa gagctttacc catattcacc
     2641 ttacctaatt tgattcttcc aggaatgcgt cgcacgttga taaaccttcc actggcaacg
     2701 agttaccgat atggactcgt tctctgcgga tatcaacccc ttgtcgtgac tctgttcacg
     2761 aaaattcagg tgactgagtt aacaggtctt ggcctacagg cgctatagca cgacggaatt
     2821 ttggtcgaat gtcaagcaaa tctattgcca gattgacgaa agcgtcacac tgtacctgat
     2881 aaaatccgat acgcaacttt attagcatgc aaaatccaaa gttgccagta aggcgctctt
     2941 gccaccagcg tggtatattt gtctgcaagc catgcctggc gtagactaat gtccttattt
     3001 aaggagtaaa taatgaacaa agagacacaa cccatcgacc gcgaaacgct gttgaaagag
     3061 gccaacaaaa tcattcgtga gcatgaggat acgctcgcag ggattgaagc caccggcgtc
     3121 acgcagcgta atggcgtgct ggtatttacc ggcgactact ttttagatga acaaggattg
     3181 ccaacagcca aaagcaccgc ggtgtttaat atgttcaaac acctggccca tgtgctttca
     3241 gaaaaatatc atctggttga ttaaagcaaa acgcgaggtc ttagcctcgc gtttgtcttt
     3301 tacatcaatg gcttttgacc ccgctgccac cagcgtagca acaatcgatc ggcgctctct
     3361 gccgcgctgc cggtgaatcg gtcaatgagt cgtttacgct gcatatagcg tacattaacc
     3421 acttcacggc cttccatcag gctaagaata acttcatcac tggtgttgat ttcatcaacc
     3481 aggcctttct ctaccgcctg ttgtccgtac cagtgttcac ctgttgccac ctgttcaata
     3541 tccagagacg gacgcatacg cttcacaaaa tctttaaaca actgatgcgt ttcgttcaac
     3601 tcttcgcgga atttctcccg cccttcttcg gtattttcac ccagcaacgt cagagtacgc
     3661 ttatactgcc cggcagtgtg cagttcgata tcaatatatt tgcttttcag gaagcggtta
     3721 aagttgggca tttgcgccac caccccaatg gaacccacaa tagcaaacgg tgcggaaaca
     3781 attttgtccg ccacacaggc catcatgtaa ccgccgctgg cagcgacttt gtctaccgta
     3841 acagttaaag gaatgttttt atcacgcaga cgctgcagct gcgaagccgc caacccgtaa
     3901 ccatgcacca tgccaccagg gctttccaga cggagcacaa cctgatcctg cggtttgaat
     3961 gctgcgagta ccgccgttat ctcttcacgt agcgagttca cttcatgggc gtccatgctg
     4021 cctttaaaat ccagcaccca gacgcgaggt ttactgtcag ttgccacctc gcccagtttg
     4081 gctttcgctt ttgctgcttt cgcttcttgc ttgtgctttt tcttctgcgc tttgtgccac
     4141 tgtttttgct gatgtgagcc catcagcgcc gcggccagtt cttctttcat ctccttatac
     4201 tgttcgctga gattgttgac ccgtaactcg ccacgctggc gtttattacg ttgagcaaca
     4261 ttgacaataa tggcggcaat cgccgcaatc gctagcacaa cggtaacgat tttcgccaaa
     4321 aacaaaccat attcagacaa caattccacg agtccccacc ttggttaaac aacgctttgg
     4381 ctcacagttt acaacaggca tgttaagccg tctcgcagag atgttattgc agcgaaagta
     4441 tgacaaagta tccattttgc ggtgttatcg ccccaggctg attgaaatgc ccttctgttt
     4501 caggcataaa gccccaaagt cataaagtac actggcagcg cgacagacaa cgtcgttgag
     4561 gagtagccat gcattaccag ccaaaacaag atttactcaa tgatcgcatt atcctggtga
     4621 cgggagccag cgatgggatt ggtcgtgaag ccgcgatgac gtatgcacgc tatggtgcga
     4681 cagtgattct gttgggccgt aatgaagaaa aattacgtca ggtagccagc cacataaacg
     4741 aagaaactgg gcgtcagcca cagtggttta ttctcgattt gctgacctgc acgtccgaaa
     4801 attgccaaca actggcacag cgcattgtcg ttaattatcc gcgtctggat ggtgttttgc
     4861 ataatgccgg attgctcggc gatatttgcc caatgagcga acaaaatccg caggtctggc
     4921 aggacgtcat gcaggtcaac gttaatgcca cctttatgct cacccaggca ctgcttcctt
     4981 tattactcaa atcggacgcc ggttcactgg tctttacttc atcaagcgtt ggacgtcagg
     5041 gacgagctaa ctggggtgca tatgcagcgt cgaaatttgc caccgaaggg atgatgcagg
     5101 tactggccga tgaatatcag cagcgcctgc gcgtcaactg cattaaccca ggcggtacgc
     5161 gcaccgcaat gcgcgcaagc gccttcccga ccgaagatcc acagaaactt aaaacacccg
     5221 ctgatatcat gccgctctac ctctggctga tgggcgatga cagccgccgt aaaaccggca
     5281 tgacctttga cgcccaaccg ggccgtaaac caggaatttc ccaatgagtg atgaacgcta
     5341 ccaacagcgt cagcagcgag tgaaagaaaa agtagatgct cgtgtggcac aggccccagg
     5401 atgaacgcgg tattatcatc gtctttaccg gcaatggaaa aggcaaaacc accgcggcat
     5461 ttggtacggc aacacgcgca gttggtcacg gaaaaaaagt gggcgtcgtg cagtttatta
     5521 aaggcacctg gcctaatggc gaacgcaatc tgctggaacc acatggcgtt gagtttcagg
     5581 tgatggcaac gggctttacc tgggatacac aaaaccgcga gtctgatacc gccgcctgcc
     5641 gcgaagtctg gcaacatgca aagcggatgc ttgctgattc ctcactggat atggttttgc
     5701 ttgatgaact gacgtatatg gtggcgtatg actatttgcc actggaagaa gtggtgcagg
     5761 cgttaaatga acgtccacat caacagacgg tgattatcac gggtcgaggc tgtcatcggg
     5821 atattcttga actggcggat acggtaagtg agctgcgccc catcaaacat gcgtttgatg
     5881 ccggtgttaa ggcgcaaata gggattgatt attaatctgt aaaaatgccg gatatgacgt
     5941 ccggcatact ttgattaacc gttattacgc ccgccagaac gacgaccgcc gctcacctga
     6001 ctgtgacgtt tcaccgcacg acgaatctga ttagccttca tgcgacgacg gtctttttct
     6061 acagcgactt tagagctggt ttctggcgga agctccacca gttcgcgcag atagttagtc
     6121 tgggcgaggt ccagctccgt ccagccaccg cgtggcagac cttttggcag tgggatatca
     6181 ccgtaacgaa cacggatcag gcggctcacc tgcacaccaa ccgcttccca cagacgacga
     6241 acttcacggt tacggccttc ggtcagagtc acgttgtacc actggttgat cccttcgccg
     6301 ccgctgaact tgatggtttt aaaagctgcc ggaccatctt ccagttgcac gccacgactc
     6361 aaatcacgca gtttcgcgtc gtcaacctga ccaaatacac gcacggcgta ttcacgttca
     6421 acttcacggc ttggatgcat taaacggttt gccagttcac catcggtggt gaacagcagc
     6481 agaccacagg tgttaacgtc cagacgacct acggcaatcc agcgtgcgcc acgcagcttt
     6541 ggcagacggt caaacaccgt cggacgccct tccggatcgt tacgggtaca caactcacct
     6601 tccggtttgt aataggccag aacgcgacaa atttgttcag cggactcacg taccgaaatc
     6661 agatgaccat cgatacgaat tttcagccca ggggtaactt caacgcgatc gccgagttta
     6721 gcaattttgc catcaacact cacacgaccg gcttcaataa tagattcgat ttcacggcgg
     6781 gagccgtggc cagcgcgggc cagcactttc tgtaattttt cgctcataga gcttcctttt
     6841 ggtgtcgcct tcccaggcgt ctgatatgct tttgtgcaac agtgagtcag aacttacatc
     6901 tgactcactg atagtaaagt ctgtttgaac tcttcaggcc ttaagtaaac gactcgacgt
     6961 ttatcggcct ggctttgagg cgcaacatac tacactatct ttatggttaa agcttgtttc
     7021 gattaacatc agcttaatta ccagccacca ccgcctccgc cacccgcgcc gccgccagca
     7081 ccaccaccgg aataacttcg ccccaacaaa ctggtattgg ccccgcaact taggtgcgtc
     7141 tgtaatgaga ataaaacgcc gttacgggca atctctggag cgcgaatcgc cgaagaaagc
     7201 gaaggggcaa tgcgtaacgc ccattccctc cccaaatttg ccgatatagc ccaacctagc
     7261 aacgcgtaat ccagtttccg tgtttcccct ttccgccgac gtccactgtg tgttgctgcc
     7321 tctttacgcg caagatagtt aacgatagct tcaccgcggg cataacgttg ctgcccagtt
     7381 tgggtaaact ttggcagaaa aatatacccc atgccagtga gataaccggt caggcaaaca
     7441 gcgctaaaat aacccgctgg catataccag taaaagaata catctccgac attgatatag
     7501 agagaataga aggccacgcc tgaacaaatc agtggtaaaa acaaagtgat gattgcacga
     7561 ttaacgcaag gcttgtcatc atcgcgggca aacaaaagct caagggaaca aaataaaaaa
     7621 ggaataatcc agcagatcat aataaacata tcgcctacca gactcattcc tgcccaacct
     7681 acgggactca ggttaccgta gagaatgatc gccaatattg ccagtccacc ccattgcaaa
     7741 aacgtatcag gccggtaaaa caaagacttt ttacgcggca gataaaattt ctgcatccgt
     7801 aaaaatgccc tgcgtaagac tctgttgcgt ctccccgtaa gaacgacctt atcgccttta
     7861 cgaaataacg cttccagtaa taactgctct gcgggcagta atacctcatt atttctggtc
     7921 gcgccttcat taacacaaat caggatttgc tggttcttat catactgatc ttccagatga
     7981 atttttccct tcacgatcag atttacgata tcggcacaga aacctttatc gtcgtaaacc
     8041 agcttcgcat cgagacgtaa catgccgggc gtgtaatctg gcggaatgac atcggtttca
     8101 atcacatcta ccggcgtaaa ttgcggcctg cgcttccaga gatataacca tccacaaacc
     8161 aggaatagac acggaaacca aattaacaac gatgacgtgg agggtaaaag aagatggctg
     8221 aaaatattcg tcgtttgtgg agccggggca ttgctaagta acgcgtgagg ccagcggtag
     8281 agtaccgtga aatcttcacg ataaaacgga tcgcgggatt caattcttcc gtcctcaagg
     8341 atgcggccat ttcgatatgt gtcgccctct tctccagtaa agagatcgat ttcgctaaat
     8401 ggattacccg cgatatcagg gaacttgagt gaaaatcggg tcttatagat ttcaaacggc
     8461 cagtggttac cagtcacgtt ccagattaac agatctgaat ctccctcacg caggaaagca
     8521 tttttaacct gataatgaat ttgataatga tagtcgcctt gaggcaagaa acgttgttta
     8581 tcgcccacga caatactcat taacccgata tgccagtcaa gatgccaggg ttcaggaata
     8641 ccatcacgag tgacgccaac aatatgatag ttcagcagtg cagcgtcccc atcaggctgc
     8701 atccagagtc gtggtaaatc acgataaaat ccgtgcttaa tctgctgatt tcgcgcctga
     8761 aaatgaatat tttctttgat gtcgagcacc ccatccggtt gcagagtggc ttcaacatca
     8821 aagagcgaga tcccttcttc ataagaaggg atctccgggt tttctgcata acttagagat
     8881 gatacaaaca gccctgctat caacagcaaa atgcagcgaa acttccctgc catagtcttc
     8941 catccgtgga acgcaatttg ttataagaaa ggcttcacat cacccacgcc ttcacgcacc
     9001 acgaccggcg tatcatcggt aagatcaatg accgttgtcg gtttctgtcc gagatagccg
     9061 ccgtggataa tcaaatccac ctgtttttcc agacggtctt taatttcttc tggatcggat
     9121 tcggtaaatt cgctgcctgg cagcattagc gaagtggaaa gcatcggttc gcccagtgct
     9181 tcaagcaacg cctgcgcaat cgggttagaa ggcacacgca taccgatggt tttgcgtttt
     9241 tcctgcaaca gacggcgtgg cacctccttc gtccccttca ggatgaaggt atagttgccc
     9301 ggcgtgttgt ttttcattaa acgaaacgca acgttatcca cgaatgaata ggtcgaaagt
     9361 tcagaaagat cgcgacacat cagggtaaag ttgtgaccgt ccggcagctg gcgaatacga
     9421 caaatacgct ccatcgcgtt tttatcttca attttacagc cgagcgcata gccggaatca
     9481 gttggataaa caatcacccc gcctttacgc acgatctcca ccgcctggtt gatcagacgt
     9541 tgctgcgggt tatcaggatg aatataaaaa aactggctca taattccctc tctgtggtgt
     9601 tctgcggctg ttcccatagc tgccagacgc cttcaacgcc tgcgggcagc cagagtttac
     9661 gacccagttc gatccacggg catggctgat gaaaatcaga tccttgtgat gcccataaat
     9721 gatgctgacg cgcaagtgtc gtcaactggg tgcgttcatt gggcgattgc tggcactgcg
     9781 cgacttccat cgcgtcgccg tggtgttcgg caaaatgcgc aaccaatctt ttcagccatt
     9841 tagcactaag attgtaccgc ccaggatgag ctagcaccgc cttaccgcca gaatgatgaa
     9901 tgacatcaat agcttgttct attgtacacc actgtggcgg aacgtatccg gttttcccgc
     9961 gcgccagata ctttttaaag acatctgcca ttgaactggc tttgccgcac tcaacaagga
    10021 aacgcgcaaa atgaccgcgc gtcactgcgc cgccctgcgc cagtcgttgt gcgccttcca
    10081 gcgccccagg tatttgcgct ttttcaagtc tttcggcaat ttgctgcgcc cgctgattgc
    10141 gccgttctgt ctgctgcgcg aggaactcac acattagcgg atgagtaata tcaatattca
    10201 gcccgacaat atgaatttca tgattttccc agaccgtgga aatttccacg ccgggaataa
    10261 gattcagcgc cagtcccgaa cgtgaaattt cctctcttgc tggcgcgatt gcagctgtgg
    10321 tgtcatggtc ggtgatcgcc agggtgccga cgcgcatctc gactgcacgg tgcaccaatg
    10381 cttctggcgt caggcagcca tcggaagctg tggtatggct gtgcaggtcg taaatcactg
    10441 cgtaattcgt gtcgctcaag gcgcactccc gttctggata atgttttttg cgccgacatc
    10501 ataacggttc tggcaaatat tctgaaatca gctgttgaca attaatcatc gaactagtta
    10561 actagtacgc aagttcacgt aaaaagggta tcgacaatga aagcaatttt cgtactgaaa
    10621 gcttggtggc gcacttcctg aaacgggcag tgtattcacc atgcgtaaag caatcagata
    10681 cccagcccgc ctaatgagcg ggcttttttt tgaacaaaat tagagaataa caatgcaaac
    10741 acaaaaaccg actctcgaac tgctaacctg cgaaggcgct tatcgcgaca acccgactgc
    10801 gctttttcac cagttgtgtg gggatcgtcc ggcaacgctg ctgctggaat ccgcagatat
    10861 cgacagcaaa gatgatttaa aaagcctgct actggtagac agtgcgctgc gcattacagc
    10921 attaggtgac actgtcacaa ttcaggcact ttccggcaac ggcgaagccc tgctgacact
    10981 actggataac gccctgcctg cgggtgtgga aaatgaacaa tcaccaaact gccgtgtgct
    11041 gcgcttcccc cctgtcagtc cactgctgga tgaagacgct cgcttatgct ccctttcggt
    11101 ttttgacgct ttccgtttat tgcagaatct gttgaatgta ccgaaggaag aacgagaagc
    11161 aatgttcttc ggcggcctgt tctcttatga ccttgtggcg ggatttgaag atttaccgca
    11221 actgtcagcg gaaaataact gccctgattt ctgtttttat ctcgctgaaa cgctgatggt
    11281 gattgaccat cagaaaaaaa acacccgcat tcaggccagc ctgtttgctc cgaatgaaga
    11341 agaaaaacaa cgtctcactg ctcgcctgaa cgatcttcgc cagcagctga ccgaagccgc
    11401 gccgccgctg ccggtggttt ccgtgccgca tatgcgttgt gaatgtaacc agagcgatga
    11461 agagttcggt ggcgtggtgc gtttgttgca aaaagcgatt cgcaccggag aaattttcca
    11521 ggtggtgccg tctcgccgtt tctctctgcc ctgcccgtca ccgctggcgg cctattacgt
    11581 gctgaaaaag agtaatccca gcccgtacat gttttttatg caggataatg atttcaccct
    11641 gtttggcgcg tcgccggaaa gttcgctcaa gtatgacgcc accagccgcc agattgagat
    11701 ctacccgatt gccggaacac gcccgcgcgg tcgtcgcgcc gatggttcac tggacagaga
    11761 cctcgacagc cgcatcgaac tggaaatgcg taccgatcat aaagagcttt ctgaacatct
    11821 gatgctggtg gatctcgccc gtaatgatct ggcacgcatt tgcacccccg gcagccgcta
    11881 cgtcgccgat cttaccaaag ttgaccgtta ctcttacgtg atgcacctgg tctcccgcgt
    11941 ggtcggtgag ctgcgccacg atctcgacgc cctgcacgct taccgcgcct gtatgaatat
    12001 ggggacgtta agcggtgcgc cgaaagtacg cgccatgcag ttaattgccg aggcggaagg
    12061 tcgtcgccgc ggcagctacg gcggcgcggt aggttatttc accgcgcatg gcgatctcga
    12121 cacctgcatt gtgatccgct cggcgctggt ggaaaacggt atcgccaccg tgcaagccgg
    12181 tgctggcata gtccttgatt ctgttccgca gtcggaagcc gacgaaaccc gtaataaagc
    12241 ccgcgctgta ctgcgcgcta ttgccaccgc gcatcatgca caggagactt tctgatggct
    12301 gacattctgc tgctcgataa tatcgactct tttacgtaca acctggcaga tcagttgcgc
    12361 agcaatgggc ataatgtggt gatttaccgc aaccatattc cggcgcagac cttaattgaa
    12421 cgcctggcga cgatgagcaa tccggtgctg atgctttctc ctggccccgg tgtgccgagc
    12481 gaagccggtt gtatgccgga actcctcacc cgcttgcgtg gcaagctgcc cattattggc
    12541 atttgcctcg gacatcaggc gattgtcgaa gcttacgggg gctatgtcgg tcaggcgggc
    12601 gaaattctcc acggtaaagc ctccagcatt gaacacgacg gtcaggcgat gtttgccgga
    12661 ttaacaaacc cgctgccagt ggcgcgttat cactcgctgg ttggcagtaa cattccggcc
    12721 ggtttaacca tcaacgccca ttttaatggc atggtgatgg cagtacgtca cgatgcggat
    12781 cgcgtttgtg gattccagtt ccatccggaa tccattctta ctacccaggg cgctcgcctg
    12841 ctggaacaaa cgctggcctg ggcgcagcgg aaactagagc caaccaacac gctgcaaccg
    12901 attctggaaa aactgtatca ggcgcagacg cttagccagc aagaaagcca ccagctgttt
    12961 tcagcagtgg tacgtggcga actgaaaccg gaacaactgg cggcggcgct ggtgagcatg
    13021 aaaattcgcg gcgagcaccc gaacgagatc gccggagcag caaccgcgtt actggaaaac
    13081 gccgcgccgt tcccgcgccc ggattatctg tttgccgata tcgtcggcac cggtggtgac
    13141 ggcagtaaca gcatcaatat ttccaccgcc agtgcgtttg tcgccgcggc ctgtgggctg
    13201 aaagtggcga aacacggcaa ccgtagcgtc tccagtaaat ctggttcgtc cgatctgctg
    13261 gcggcgttcg gtattaatct tgatatgaat gccgataaat cgcgccaagc gctggatgag
    13321 ttaggcatat gtttcctctt tgcgccgaaa tatcacaccg gatttcgcca tgcgatgccg
    13381 gttcgccagc aactgaaaac ccgcactctg ttcaacgtgc tgggaccatt gattaacccg
    13441 gcgcatccgc cgctggcgtt aattggtgtt tatagtccgg aactggtgct gccgattgcc
    13501 gaaaccttgc gcgtgctggg gtatcaacgc gcggcggtag tgcacagcgg cggtatggat
    13561 gaagtttcat tacacgcgcc gacaatcgtt gccgaactgc atgacggtga aattaagagc
    13621 tatcagctaa ctgcagaaga ttttggcctg acaccctacc accaggagca actggcaggc
    13681 ggaacaccgg aagaaaaccg tgacatttta acacgcttgt tacaaggtaa aggcgacgcc
    13741 gctcacgaag cagccgtcgc ggcgaatgtc gccatgttaa tgcgcctgca tggccatgaa
    13801 gatctgcaag ccaatgcgca aaccgttctt gaggtactgc gcagtggttc cgcttacgac
    13861 agagttaccg cactggcggc acgagggtaa atgatgcaaa ccgttttagc gaaaatcgtc
    13921 gcagacaagg cgatttgggt agaaacccgc aaacagcagc aaccgctggc cagttttcag
    13981 aatgaggttc agccgagcac gcgacatttt tatgatgcgc tacagggtgc acgcacggcg
    14041 tttattctgg agtgcaagaa agcgtcgccg tcaaaaggcg tgatccgtga tgatttcgat
    14101 ccggcacgca ttgccgccat ttataaacat tacgcttcgg caatttcggt gctgactgat
    14161 gagaaatatt ttcaggggag ctttgatttc ctccccatcg tcagccaaat cgccccgcag
    14221 ccgattttat gtaaagactt cattatcgac ccttaccaga tctatctggc gcgctattac
    14281 caggccgatg cctgcttatt aatgctttca gtactggatg acgaccaata tcgccagctt
    14341 gcagccgtcg ctcacagtct ggagatgggg gggctgaccg aagtcagtaa tgaagaggaa
    14401 ctggagcgcg ccattgcatt aggggcaaag gtcgttggca tcaacaaccg cgatctgcgt
    14461 gatttgtcga ttgatctcaa ccgtacccgc gagcttgcgc cgaaactggg gcacaacgta
    14521 acggtaatca gcgaatccgg catcaatact tacgctcagg tgcgcgagtt aagccacttc
    14581 gctaacggtt ttctgattgg ttcggcgttg atggcccatg acgatttgca cgccgccgtg
    14641 cgccgggtgt tgctgggtga gaataaagta tgtggcctga cgcgtgggca agatgctaaa
    14701 gcagcttatg acgcgggcgc gatttacggt gggttgattt ttgtcgcgac atcaccgcgt
    14761 tgcgtcaacg ttgaacaggc gcaggaagtg atggctgcgg caccgttgca gtatgttggc
    14821 gtgttccgca atcacgatat tgccgatgtg gtggacaaag ctaaggtgtt atcgctggcg
    14881 gcagtgcaac tgcatggtaa tgaagatcag ctgtatatcg atacgctgcg tgaagctctg
    14941 ccaacacatg ttgccatctg gaaagcatta agcgtcggtg aaaccctgcc cgcccgcgag
    15001 tttcagcacg ttgataaata tgttttagac aacggccagg gtggaagcgg gcaacgtttt
    15061 gactggtcac tattaaatgg tcaatcgctt ggcaacgttc tgctggcggg gggcttaggc
    15121 gcagataact gcgtggaagc ggcacaaacc ggctgcgccg gacttgattt taattctgct
    15181 gtagagtcgc aaccgggcat caaagacgca cgtcttttgg cctcggtttt ccagacgctg
    15241 cgcgcatatt aaggaaagga acaatgacaa cattacttaa cccctatttt ggtgagtttg
    15301 gcggcatgta cgtgccacaa atcctgatgc ctgctctgcg ccagctggaa gaagcttttg
    15361 tcagcgcgca aaaagatcct gaatttcagg ctcagttcaa cgacctgctg aaaaactatg
    15421 ccgggcgtcc aaccgcgctg accaaatgcc agaacattac ggctgggacg aacaccacgc
    15481 tgtatctgaa gcgcgaagat ttgctgcacg gcggcgcgca taaaactaac caggtgctcg
    15541 gtcaggcttt actggcgaag cggatgggta aaaccgaaat catcgccgaa accggtgccg
    15601 gtcagcatgg cgtggcgtcg gcccttgcca gcgccctgct cggcctgaaa tgccgtattt
    15661 atatgggtgc caaagacgtt gaacgccagt cgcctaacgt ttttcgtatg cgcttaatgg
    15721 gtgcggaagt gatcccggta catagcggtt ccgcgacgct gaaagatgcc tgtaacgagg
    15781 cgctgcgcga ctggtccggc agttatgaaa ccgcgcacta tatgctgggc accgcagctg
    15841 gcccacatcc ttatccgacc attgtgtgtg agtttcagcg gatgattggc gaagaaacga
    15901 aagcgcagat tctggaaaga gaaggtcgcc tgccggatgc cgttatcgcc tgtgttggcg
    15961 gcggttcgaa tgccatcggc atgtttgcag atttcatcaa cgaaaccaac gtcggcctga
    16021 ttggtgtgga gccgggtggt cacggtatcg aaactggcga gcacggcgca ccgctaaaac
    16081 atggtcgcgt gggcatttat ttcggtatga aagcgccgat gatgcaaacc gaagacgggc
    16141 agattgaaga atcttactcc atctccgccg gactggattt cccgtctgtc ggcccacaac
    16201 acgcgtatct taacagcact ggacgcgctg attacgtgtc tattaccgat gatgaagccc
    16261 ttgaagcctt caaaacgctg tgcctgcacg aagggattat cccggcgctg gagtcttccc
    16321 acgccctggc ccatgcgctg aaaatgatgc gcgaaacccc ggaaaaagag cagctactgg
    16381 tggttaacct ttccggtcgc ggcgataaag acatcttcac cgttcacgat attttgaaag
    16441 cacgagggga aatctgatgg aacgctacga atctctgttt gcccagttga aggagcgcaa
    16501 agaaggcgca ttcgttcctt tcgtcacgct cagggatccg ggcattgagc agtcattgaa
    16561 aattatcgat acgctaattg aagccggtgc tgacgcgctg gagttaggta tccccttctc
    16621 cgacccactg gcggatggcc cgaccattca aaacgccact ctgcgcgcct ttgcggcagg
    16681 tgtgactccg gcacaatgtt ttgaaatgct ggcactgatt cgccagaaac acccgaccat
    16741 tcccattggc ctgttgatgt atgccaatct ggtgtttaac aaaggcattg atgagtttta
    16801 tgcccagtgc gagaaagtcg gcgtcgattc ggtgctggtt gccgatgtgc cagttgaaga
    16861 gtccgcgccc ttccgccagg ccgcgttgcg ccataatgtc gcacctatct tcatctgccc
    16921 gccaaatgcc gatgacgacc tgctgcgcca gatagcctct tacggtcgtg gttacaccta
    16981 tttgctgtca cgagcgggcg tgaccggcgc agaaaaccgc gccgcgttac ccctcaatca
    17041 tctggttgcg aagctgaaag agtacaacgc tgcacctcca ttgcaggggt ttggtatttc
    17101 cgccccggat caggtaaaag cagcgattga tgcaggagct gcgggcgcga tttctggttc
    17161 ggccattgtt aaaatcatcg agcaacatat taatgagcca gagaaaatgc tggcggcact
    17221 gaaagttttt gtacaaccga tgaaagcggc gacgcgcagt taatcccaca gccgccagtt
    17281 ccgttggcgg cattttaact ttctttaatg aagccggaaa aatcctaaat tcatttaata
    17341 tttatctttt taccgcttcg cttacccctg tcgaacgtca acttacgtca tttttccgcc
    17401 caacagtaat ataatcaaac aaattaatcc cgcaacataa caccagtaaa atcaataatt
    17461 ttctctaagt cacttattcc tcaga
//
LOCUS       DAJVUJ010000085        17399 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.085, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000085 DAJVUJ010000000
VERSION     DAJVUJ010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17399
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.085"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(<1..218)
                     /locus_tag="OQQ48_002519"
     CDS             complement(<1..218)
                     /locus_tag="OQQ48_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BAX inhibitor (BI)-1/YccA family protein"
                     /protein_id="HCS1953250.1"
                     /translation="MESYSQNSNKLDFQHETRILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPTMYVAIGLLLVCMYGLSK"
     gene            complement(424..1128)
                     /locus_tag="OQQ48_002520"
     CDS             complement(424..1128)
                     /locus_tag="OQQ48_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCS1953251.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(1265..1717)
                     /gene="moaE"
                     /locus_tag="OQQ48_002521"
     CDS             complement(1265..1717)
                     /gene="moaE"
                     /locus_tag="OQQ48_002521"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HCS1953252.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFAGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(1719..1964)
                     /gene="moaD"
                     /locus_tag="OQQ48_002522"
     CDS             complement(1719..1964)
                     /gene="moaD"
                     /locus_tag="OQQ48_002522"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HCS1953253.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHMAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            complement(1957..2442)
                     /gene="moaC"
                     /locus_tag="OQQ48_002523"
     CDS             complement(1957..2442)
                     /gene="moaC"
                     /locus_tag="OQQ48_002523"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HCS1953254.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(2445..2957)
                     /gene="moaB"
                     /locus_tag="OQQ48_002524"
     CDS             complement(2445..2957)
                     /gene="moaB"
                     /locus_tag="OQQ48_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HCS1953255.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(2979..3968)
                     /gene="moaA"
                     /locus_tag="OQQ48_002525"
     CDS             complement(2979..3968)
                     /gene="moaA"
                     /locus_tag="OQQ48_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HCS1953256.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(3956..4100)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            4365..5273
                     /gene="yvcK"
                     /locus_tag="OQQ48_002526"
     CDS             4365..5273
                     /gene="yvcK"
                     /locus_tag="OQQ48_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HCS1953257.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     gene            complement(5465..7486)
                     /gene="uvrB"
                     /locus_tag="OQQ48_002527"
     CDS             complement(5465..7486)
                     /gene="uvrB"
                     /locus_tag="OQQ48_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HCS1953258.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVETQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(8065..8742)
                     /gene="bioD"
                     /locus_tag="OQQ48_002528"
     CDS             complement(8065..8742)
                     /gene="bioD"
                     /locus_tag="OQQ48_002528"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCS1953259.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            complement(8735..9490)
                     /gene="bioC"
                     /locus_tag="OQQ48_002529"
     CDS             complement(8735..9490)
                     /gene="bioC"
                     /locus_tag="OQQ48_002529"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HCS1953260.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHAELQRRSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            complement(9477..10631)
                     /gene="bioF"
                     /locus_tag="OQQ48_002530"
     CDS             complement(9477..10631)
                     /gene="bioF"
                     /locus_tag="OQQ48_002530"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HCS1953261.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEQFGVGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFVHNDVTHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYNTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            complement(10628..11668)
                     /gene="bioB"
                     /locus_tag="OQQ48_002531"
     CDS             complement(10628..11668)
                     /gene="bioB"
                     /locus_tag="OQQ48_002531"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HCS1953262.1"
                     /translation="MAHRPRWTLSQVAELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPNERDMPYLEQMVQGVKDLGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            11755..13044
                     /gene="bioA"
                     /locus_tag="OQQ48_002532"
     CDS             11755..13044
                     /gene="bioA"
                     /locus_tag="OQQ48_002532"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HCS1953263.1"
                     /translation="MTKDDLAFDQRHIWHPYTSMTSPLPVYPVVSAEGCELILSDGRR
                     LIDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEVRQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHNLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            13103..13579
                     /locus_tag="OQQ48_002533"
     CDS             13103..13579
                     /locus_tag="OQQ48_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HCS1953264.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVEHIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            13731..15014
                     /locus_tag="OQQ48_002534"
     CDS             13731..15014
                     /locus_tag="OQQ48_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HCS1953265.1"
                     /translation="MNTFSVSRLALALAFSVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(15155..17392)
                     /locus_tag="OQQ48_002535"
     CDS             complement(15155..17392)
                     /locus_tag="OQQ48_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HCS1953266.1"
                     /translation="MFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSSHNTSGNMD
                     KLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGTINGDDHVF
                     GLSAAQRYGGIFVPPHIAIIHQYMREMMAGGGKMILGSDSHTRYGALGTMAVGEGGGE
                     LVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVKNKVMEFVG
                     PGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQLNPQPMAYY
                     DGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAHGKAKLSLL
                     DKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPSSQPVFMDL
                     AKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREGSKPANGQM
                     SAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQGFVKGATQQ
                     PLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRSNPIGLAEF
                     TLSRRDPGYVGRSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDPLQTEIGSMV
                     YAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPTFEV
                     GDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIKAGSLINFN
                     KNRQM"
BASE COUNT         4054 a         4714 c         4420 g         4211 t
ORIGIN      
        1 tccttactta agccatacat acaaactaat aataacccta tggcgacata cattgttggt
       61 gaatcatatt tggttgccgt aatttcgaga tacttagctc cccaggccag tcctgcggtt
      121 gccaccaaac ctaaagcggt aatgagccat ataccgttta atatcctggt ctcgtgctga
      181 aaatctaatt tattgctgtt ttgtgagtat gattccattc ttttagttgt ccttagaaaa
      241 aatattaata ttcaccatca ataaatttaa acccacgcat acaaaatact tttgtattta
      301 tggatacgga aaacgcttcc gctcggccat aaggcgtatt ttatacctgg tacttgttaa
      361 gtgtcgtttt tagtaagaaa agaaatcccg acacatggcc gggatattga tggtgaaaaa
      421 gaattaacgg cggttgccaa aaattcgcag caacatcagg aacaggttga tgaagtccag
      481 atacaaggtt aacgcgccaa gaatggaata tttgcgcagg ttcgacgtgt cgcgggtatc
      541 aatctgctca cccatatttt tcaatttctg agtgtcatac gccgtcaatc cgacaaagac
      601 aatcacgccg atgtaggtaa ctgcccacat caatgcttcg cttttcagcc agaagttgac
      661 cagcgatgcc agcacaatgc cgattaacgc cataaacagc atattgccaa agccacttaa
      721 atcgcgcttc gtggtgtaac cgtacaggct cattgcgccg aacatcccgg cagtaacgac
      781 gaaagtactg gcgatagaag cagcggtata gacaatgaat atactggaaa gcgtaagacc
      841 cgtcagcgcc gaataaagca taaagagcat cgtcgttaca cctgcgctca gcttttgaat
      901 catcgctgat aacacaataa ccaatgctaa ttgcgcgatg atcagaccga ttaaaaagac
      961 acggttagtg aacaacagct ccatcacggc cgcggaatta gccgcatacc aggcaacaaa
     1021 tgcggtcagc aacaagccaa cggtcatcca gccatagact tgcgccatat aagtttgcaa
     1081 gccagcccgg ggttgtacga ttgaatcaga acgtgggaat ctgtccatga cgatctcctg
     1141 aagatataag gaatatctta aggatactgc aaaatgatga ggctgtgcat cgacgcagcg
     1201 taaacgcatg tactgagcgg tgaaattgcc ggacgcagcg gtgccttatc cggctaacaa
     1261 aaaactacca gcgttttgcc gcctgctgat cgctctcccg agcttcaacc cagcggtcgc
     1321 cttccggcgt ggcttcgcgc ttccagaacg gtgcgcgggt tttgagataa tccataataa
     1381 actgcccggc ttcaaacgca ctgctgcgat gcgcactggt gacaccggca aaaacgattt
     1441 catcgcccgg ccataattcc ccgatgcggt gaatcacagt gacgcgcccc agcggccagc
     1501 ggttacgcgc ttcatcaaca atttctgcca gtgctttttc agtcattccc ggatagtgtt
     1561 cgagggttaa tgctttgacg ctgtcgccca ggttatggtt gcgcacctta ccagtaaagg
     1621 tgactaccgc accgtcttcg tcacgctccg ccagccacgg gtactcttct cctacgctga
     1681 acggctgcgg accaacaaca attttggttt ctgccatctt aacctccggt taccggcggg
     1741 aagaaagcta cttcgtcgcc gtcagtcagc ggatggtcaa aactcaccag cgtctggttg
     1801 acggcagcca gtaatttgcc atcttccagc gccagcgccc agcgatcgct ctgcgcagcc
     1861 atgtgctggc gtaacgcttc aacagttggg aaatccgcag ccacttcggt tgcatctgtt
     1921 cccaccaact cgcgcacctg cgcgaaaaaa agaactttaa tcatccgctt ccaccttaaa
     1981 gtcacccgac ttgccgccgc tcttcgccag caaacgtacc ggaccaatca ccatatcttt
     2041 ttgcaccgct ttgcacatgt cataaatggt cagcgccgcc acggaggccg cggttaatgc
     2101 ttccatttcg acaccggttt tcccggtcag gcggcataag gtttctatac gcacccgatt
     2161 gtgctccggc tcggcctgta aattgacttc aactttgctg agcatcagcg gatgacagag
     2221 cgggatcaga tcccaggtgc gttttgccgc ctgaataccg gcaatacgcg cagtggcaaa
     2281 tacgtcgcct ttgtggtggc gaccatcaat aatcatcgcc agcgtctcgc tgcgcatggt
     2341 gacaaaggct tccgcccgcg cttcacgcac ggtttccgct ttggcggaga catccaccat
     2401 gtgcgcttcg ccagcggcgt tgatatgggt cagttgcgac atacttattt cttcaaatgt
     2461 ggatggaaat tacacggacg cgtacgggca tccagctgcg gcgcgatgat attttcccat
     2521 gcggtacggc acgctttggt cgaacccggc atggcgaaaa tcagcgtttt gttggcgacg
     2581 cccgcaaccg cacgagattg caacgtggaa gtgccaatct cttcaaacga caacatccgg
     2641 aacacttcac caaaaccttc aacttcacgg tcgaacaacg gcagcaatgc ttcgggagcc
     2701 tgatcacctt ccgtcaggcc agtaccaccc gtaatcaata ccacttgtac atcgtcgctg
     2761 gcgatccacg cagatacctg agcgcgaata gcgtagcggt tttctttcac aatggcttta
     2821 tcgacaacgt gatggcccgc ttcttgcgcc gaatcgcgca gatagtgacc ggaggtatcg
     2881 tcttcttcac cgcgacgatt agaaaccgta agaatagcaa tacgggtcgg gataaattca
     2941 gtgcttacct gactcatctg atctctcctt ttgacgtttt agccgccaat gtacgataag
     3001 ttttgcgtaa taccggtgtt gttttgatgc aggaaatggg tctgtttctt ctcccgcagc
     3061 gccgctgaaa tacgcgcttc cagcgcctgt tgctgggtat cgtcttccag cagatcgcgc
     3121 aggttaacgc cgccttcacc aaacaggcag agatggagtt taccaatgga ggaaacgcgc
     3181 aggcggttgc aagtggcgca gaagtctttt tcatacggca tgataaggcc aatctctccg
     3241 gcgtaatctg gatggcaaaa gacttgcgcg ggaccgtcgc tgcgttgacg taattggtgg
     3301 atccagccgc gacgcagtag ctcgtcacgc agaacctgac cagagatgtg atgcttacgg
     3361 aagagctcgc tgccctcgcc cgtttccatc agttcgatga aacgcagctg gatagggcga
     3421 tgctggatcc agttcagaaa ggtgtcgagc tggtgatgat taacatcacg catcagcacg
     3481 gtattgactt tgaccttctc aaaaccggcc tcaaatgcag catcaatccc tgccatgacc
     3541 tggttgaatt tatcctgccc ggtaatagcg tgaaactggc gggcgtccag actgtcgaca
     3601 ctgacgttaa tgccagtaag tcccgcatcg cgccagttcg ccacatcgcg ttccagacgg
     3661 taaccattgg tggtgaccgc aatctggcgg atagcgtcgt tttcccgcac agcggcgatg
     3721 atatcggtaa agtcgcggcg taaagacggc tcacctcctg tcaggcgcac tttttcggtg
     3781 cccagactgg cgaaggcgcg cgtaacccgg cgaatttcat cgacggtaag aaagccttta
     3841 ttggtgacgc cgctcggttt gtagccatcc ggcaggcagt aggtgcaacg aaagttacac
     3901 acatcggtaa tcgacaggcg caagtagtaa aacttacgcg caaatgcatc agtcagttgt
     3961 gaagccatgt acacctttcc agatacggga ggcgaagtca tttcttcctt cgccctggtg
     4021 gcaatcttta cgagcattgt cacggccaaa gcaccgtatc agttgaccca ggtgcagagg
     4081 ctagagtgtt tagtggttaa gccgatacta gcgtgtaaat gagattttta ccatccacac
     4141 tttcgctatg caatcatgta tatagcgtca tgatcgtgct atttcgctac agaataaggg
     4201 aaaatggcag ttttactttg atatacatca tcagggaggc atagcgtgca tcgtccttta
     4261 gaggtagaag aaaatagttt gtatcctcaa tattggcagg ttaattgctg tttccccgca
     4321 atttgcgcta ctgtagcgcg gataatctga ctccaggaaa ctatatgcgc aatcgtacgc
     4381 tggctgatct tgatcgtgtc gttgctctcg gcggagggca tggactggga cgcgttctct
     4441 catcactttc gtctttgggt tctcgtttaa cgggtatcgt caccaccacg gataatggtg
     4501 gctcgacggg gcgtattcgc cgttcagaag gcggcattgc ctggggcgat atgcgcaact
     4561 gcctcaacca actgataacg gaaccgagcg tcgcctccgc gatgtttgaa taccgttttg
     4621 gcggcaatgg cgaactttcc gggcacaacc ttggaaactt gatgttaaag gcgctggatc
     4681 accttagcgt gcggcctctg gaagccatca atttaattcg taatctgctg aaagtggata
     4741 cgcatttgat tccaatgtca gagcatcccg tcgatctgat ggcgattgac gatcaggggc
     4801 atgaagttta cggcgaggtc aatatcgacc agttaactac gccgattcaa gagttattat
     4861 taacgccgaa tgtacccgca acgcgtgagg cagttcacgc tatcaatgaa gcggatctca
     4921 tcattattgg gcctggcagt ttttatacca gcctgatgcc aattctgctg ctgaaggaaa
     4981 tcgcccaggc attacgccgc acgccagcgc cgatggttta tatcggcaat ctggggcgtg
     5041 agttgagttt acctgcggct aatttgaagc tggaaagcaa gctggcaatt atggagcagt
     5101 atgttggtaa aaaagtcatt gatgcggtca tcgtcgggcc aaaagtggat gtctcggcgg
     5161 tgaaagagcg gattgtgatc caggaggtac tggaggccag cgatattccg tatcgtcatg
     5221 accgccagtt gttacataac gcgctggaaa aggcgttaca ggctttaggt taacggaaat
     5281 ccgaaggaaa attccggctt cctattgaag acaaagtgcg cgttgtttat gccggatgcg
     5341 gcgtgaacgc cttatccggc ctacaaaccg cgcaaattca atatattgcg gagaaaatgt
     5401 aggcctgata agcgtagcgc atcaggctgt tttccgtttg tcatcagtct tcttcgctat
     5461 cctgttacga tgcggcaata aacaactcac gcaactgatg caactggtca cgaatttgtg
     5521 ccgcttcttc gaactccaga ttctgcgcgt gttgcatcat cagcccttcc agttcatgga
     5581 ttttctgctg caacgctttc ggcgacatat ccatcggcac attatccggc tcaacaatcg
     5641 ggcgcgattt tcctctgccc ttcgctttgg ttttggcaat gttctgcccc agcgccagga
     5701 tatcgaccac tttcttgttc aagccttgcg gcgtaatgcc gtgttcctcg ttgtacttct
     5761 gctgtttctc acggcgacgt tcggtttcgc caatcgcttt cgccattgat ggggtgatct
     5821 tatcgccgta gagaatcgct ttaccgttaa cgttacgtgc cgcacgacca atggtctgga
     5881 tcaacgaacg ttcggaacgc aggaagcctt ctttgtcagc gtcgaggatc gccaccagcg
     5941 acacttccgg catatccaga ccttcgcgca gtaagttgat ccctaccagc acgtcgaact
     6001 cacccagacg caagtcgcgg ataatctcca tacgttcgac ggtgtcgata tctgagtgaa
     6061 gataacgcac gcgctcgcca tgttcttcga gatattcggt aagatcttcc gccatccgct
     6121 tggtcagtgt ggtgaccagt acgcgttcgt taattgccgc tcgctgacga atctccgaaa
     6181 gaagatcatc aacctgtgtc tccaccggcc gcacttcgat aatcgggtca agcaatccgg
     6241 ttggacgcac cacctgatcc accacatcgc cgccggattt ttccagctcg taattacccg
     6301 gcgtcgccga aacatagatg gtttgcggcg ctaatgcttc gaactcttca aacttaagcg
     6361 gacggttatc cagcgctgat ggcaggcgga agccgtactc caccagtgtc tctttacgcg
     6421 cccggtcacc gcgatacatg ccgccaattt gtggaatggt gacgtgagat tcatcgacga
     6481 ccagcagccc atcggcaggc aggtaatcaa acagcgtcgg cggtggctca cccggtccac
     6541 gaccggagag gaagcgcgag tagttttcaa tccccgaaca gtagcccagc tcgttcatca
     6601 tctccagatc aaactgggta cgctgggtca gccgctgctc ttccagcagt ttgttgtttt
     6661 ccaacagcac tttgcgtctg gcggccagtt cttctttgat ctcctccatc gcctgtacga
     6721 tgcgctcgcg cggtgtgacg tagtgcgttt tcgggtagat ggtaaaacgt ggaatagtgg
     6781 aaacaatctg cccggtcagc gggtcaaata acgacaatcg ttccacttcc tcgtcaaaca
     6841 gttccacgcg aagtgcaatg tcatccgatt ctgccgggaa gatatcaatc acctcgccac
     6901 gaacgcggaa agtaccacgc tggaatgctt gatcattacg agcgtattgc agctccgcca
     6961 gtcggcgcag aatcgcgcgc tgatcgataa tcataccgac cgtgagatgg agcatcatct
     7021 tgagatataa atcaggatcg cccagaccat aaatcgcgga aacagaggcc actacaacca
     7081 catcacgccg ctccagcatc gctttggtgg cggataaacg catctgctca atatgttcgt
     7141 taaccgaggc atctttctca atgaaagtgt cggaactcgg tacataggct tccggctgat
     7201 agtagtcgta gtaggagacg aaatactcca ccgcgttttc cgggaagaac tctttcattt
     7261 cgccatacag ctgggccgcc agcgttttgt tgggcgcaag taccatggtt gggcgctgaa
     7321 ggtcagcaat gacattggca atggtgaagg ttttccctga gccagtcacg ccaagtaacg
     7381 tctggtgcgc caggccatct tccagcccct cttcgagacg tcgaatcgcc tctggctgat
     7441 cgccagaagg tttaaaagcg gaattcagtt tgaacggttt actcatgagt cgctacctga
     7501 aggagttggg cgggcaggta tgtaatttta ctcgtcgtac ttaattatgc caacaaatta
     7561 tactggataa aaaaacagtt catcaccata atatttctga tacagcgtaa actccgctgt
     7621 caatcatgag caaaatttac tctgtggcga gataaaactc cggccttacc gggttatccc
     7681 caaagcaacg gcttttttaa catttgtcaa gatgagtgat gacagtttta tggcaagaga
     7741 tgcctgttca gtgactgcca ttgcatttat ctaaccagtt aaaaaataaa agatatttct
     7801 ttgagccgtc tttaacgcca attcgcgtgc aagccgcgta ttctctcgct tgcctcgtgt
     7861 tttctaactc taatacacat ggttatccac aggaatagtg gataactgtc tccagcccct
     7921 atcccccgcc gcctgggcaa ttcccacatt cgccgcatga ggcggaagta aaattttttt
     7981 cgtgattatt ttagaattta attggttaaa ttgcagtcaa tcgaagacgc gatctcgctc
     8041 gcaatttaac caaatacaga atggctacaa caaggcaagg tttatgtact ttccggttgc
     8101 cgcattttct ggattttctg caagccaggg gatctctccc agcagcggcg cgggaatcat
     8161 gcgggtgagc gtggtcatat attcagcgtg acgttttccc ggaggcgtaa catcgttcgc
     8221 cacccaaccc gccagagtca gtccggcgtg ttgtattgcc tgcgcagtca acatcgcgtg
     8281 attaatacag ccgagtttca caccaactac cagtatcacc ggcagttgtt cctgtgttac
     8341 ccaatctgca aaagtgaaag tgtcagaaag cggcgtaaac cagccgccag caccttccac
     8401 taacacccag tcagcctgtt gttcaagcgc gcgtaatccg gcgctcatta ccaatgattc
     8461 tatcggcctg ccctcttgcg cgctgatgat gtgcggcgaa gtgggttctg cgaaggtgta
     8521 aggatttact gttgcgtaat ccagctgcag gctgctgttg tgctgtaacg ccagcgcgtc
     8581 actattacgt aagccttccg gggtcttttc gctgccagag gcgaccggtt tataacctgc
     8641 cgtccgataa cctactgcct tcgcggcttg taaaagtgca caactggcga cggttttccc
     8701 cacttcggta tccgttccgg tgacaaaata acgtttactc acgagcaatc actcccaaaa
     8761 aaagatgata cgtcagagga tatcgcccct gttgttgcgg ccaggccagt tgcaatcgct
     8821 gcaattgcga acgcgttaat attcgcgggt cgcgcccttc atgaagatgc gtagcaccga
     8881 tgcctttcag cgaacgcatg gcactgagcg catcatcaaa ccatagtgtg atgggctgaa
     8941 tatgatgttg ataatgcacg ccgttcagcg actgttcgat ttcatctggc ggtaaaaagc
     9001 gattagcatg cggacgctcg tccaccgcct gccacgcctg atgcagttcg ggtaacgatc
     9061 cctgcaccag cgtggtaaac gccaccacgc ctccggggcg caccacccga tacagctcgc
     9121 ggagtgccgt ggataaatta ccgcaccact gcaccgcgag attgctccat gcaagatcga
     9181 acgtcgcagt cgctaacggc agggattcga tatctcccgc cagataatgg tctgcagcat
     9241 ctttctggcg ggcttgcaca agcattggcg gtgagagatc taacgccgtc acctgtgcgt
     9301 gacgctctcg ccagtggcgg ctcatccagc ctggcccaca ccccgcgtcc agtacgtggg
     9361 tgtatttacg ctgtgggagc attgccagta agacgtcagc actccggcgc tgtaactctg
     9421 cgtgttgctc atagtgcgcg gctgcccgac caaatgccgc tgcaatggct tgtttattaa
     9481 ccgttgccat gcagcacctc cagcagacgg tcgatatcct gcatttcatg cgctgcggtt
     9541 agcgttaagc gcagtcgcgc agtaccagcg ggtacggttg gcgggcgaat cgccgtgacc
     9601 cagcagcctt gctggcgcag tttttctgcc agttgtaacg cacggctgtt atcaccgacg
     9661 atcaatggct ggatggcgct gcatgaatca gcaagcgtaa acggcaaatc ctgtactccg
     9721 gcacgaaaac gcgcaatgag tgacaccagt ttttcgcgcc gtgcatcccc ctcatcacgg
     9781 cgaatgaccg ccagcgacgc acgtaatgcc tgcgcctgag cgggcggcat actggtgttg
     9841 tagataaggt ggcgggcgaa ttgcagcaga taatccgcca ccgtactgga gcaaagcacc
     9901 gctgccccgc tgacgccaaa tcctttgcca aaagtcacca ccagcaattc tggttttacc
     9961 ttttgcagcc agcagctgcc gcgcccctgc tccccgataa cgcccgtgcc gtgggcatca
    10021 tcgaccatca accagccatt gtgctgttgc gttacctgct ggatttccgc cagtggcgca
    10081 ctatcgccgt ccatgctgaa cacgccttct gtcaccacca gttgctgccc cggacagggg
    10141 gaagcaagca gtcgcgccag atgagtgaca tcgttatgaa caaaacggcg aagctgcgac
    10201 gggcttaaac tggcagcctc cagcaatgag gcatggctaa gccggtcagc gacaatgcgg
    10261 tcctcttttg ccatcatcgc ggcaatgacc gcctgattag cggcaaaacc agagataaat
    10321 agcagtgccc gcgaatagcc cagccactcg gccagttctt cttccagcgc ctgatgcgca
    10381 acgctataac cgctgacgtg accggaaccg ccgctaccga cgccaaattg ctccgcaccc
    10441 tgctgccagg cacggataat ttgcggatga tggcttaaac ctaaataatc gttactggaa
    10501 aagttcagat actggcgatc atccgccacc agccagcgtc cggctccttg cgccactgga
    10561 taacggcgac gcagggcatc ggcagcacgc cgcgcatcga gcgccgcgtt gattttctcc
    10621 tgccagctca tagtgctgcc gcgttgtaat attcgtcagt atccggggtc atcagcgcct
    10681 gttcaagacg ttgctgttgt tcgttatccc ctgccagcac ggcagtttgc tgcggattta
    10741 gccccagttt gcggaacagt tgcaggtctt tatcttcttc cggattcggc gtggtcagca
    10801 gtttgcaacc gtagaaaatc gagtttgcgc ctgccataaa gcacatcgcc tgagtctgtt
    10861 cgttcatctg ctcgcgtccg gcagaaaggc gcacgtaaga ggtcggcatc atgatccgcg
    10921 cgaccgcaat ggtgcgaata aaatcaaagg catcaacgtc atcgttatcg gcaagcggcg
    10981 tgcctttcac cttcaccagc atgttgattg gcacgctttc cggcggcgtc ggcaggtttg
    11041 ccagttgcag caataatccg gcgcgatctt ttaccgtttc gcctaagccc acaatgccgc
    11101 cagaacagac tttgatcccg gcatcgcgca ctttttccag cgtatcgagg cgttcctgat
    11161 aagtgcgggt ggtgatgata ttgccgtaaa actccggcga ggtgtcgagg ttgtggttgt
    11221 agtaatccag cccggcgttc gcgaggcgct gcgcctgaga ttcactcaac gtgcccagcg
    11281 tcatacacgc ctccagccct aaatctttta ccccctgcac catctgctcc aggtacggca
    11341 tatcgcgttc attgggattc ttccacgccg cgcccatgca aaaacgcgtc gatcccgccg
    11401 ctttcgcttt gcgcgccgac tccagcacct gttcaacttc catcaaccgc tcggcttcca
    11461 gaccggtttt atagcgcgag ctttgcgggc aatatttgca atcttccggg caagcaccgg
    11521 ttttaatcga cagcaacgtg ctgacctgca cctgacgagg atcgaaatgc tggcgatgca
    11581 cctgctgcgc ttcaaacagc agatccagca acggtttttc aaataattct gcgacttgcg
    11641 acaatgtcca gcgtgggcgg tgagccatgg ggcttctcca aaacgtgttt tttgttgtta
    11701 attcggtgta gacttgtaaa cctaaatctt ttcaatttgg tttacaagtc gattatgaca
    11761 aaggacgatc ttgcctttga ccaacgccat atctggcacc catacacatc catgacctcc
    11821 ccgctgccgg tttatccggt ggtgagcgcc gaaggttgcg agctgatttt gtctgacggc
    11881 agacgcctga ttgacggtat gtcgtcctgg tgggcggcga tccacggcta caatcacccg
    11941 cagcttaatg cggcgatgaa gtcgcaaatt gatgccatgt cgcatgtgat gtttggcggt
    12001 atcacccatg cgccagccat tgagctgtgc cgcaaactgg tggcgatgac gccgcaacca
    12061 ctggaatgcg tttttctcgc ggactccggt tccgtagcgg tggaagtggc gatgaaaatg
    12121 gcgttgcagt actggcaagc caaaggcgaa gtgcgccagc gttttctgac cttccgcaat
    12181 ggttatcatg gcgatacctt tggcgcgatg tcggtgtgcg atccggacaa ctcaatgcac
    12241 aacctgtgga aaggctatct gccagaaaac ctgtttgctc ccgccccgca aagccgcatg
    12301 gatggtgaat gggatgaacg cgatatggtt ggctttgccc gcctgatggc ggcgcatcgt
    12361 catgaaatcg cggcggtgat cattgaaccg attgtccagg gcgcaggcgg gatgcgcatg
    12421 taccatccgg aatggttaaa acgaatccgc aaaatgtgcg atcgcgaagg tatcttgctg
    12481 attgccgacg agatcgccac cggatttggt cgtaccggca aactgtttgc ctgtgaatat
    12541 gcagaaatcg cgccggacat tttgtgcctc ggtaaagcct taaccggcgg cacaatgacc
    12601 ctttccgcca cactcaccac gcgcgaggtt gcagaaacca tcagtaacgg cgaagccggc
    12661 tgctttatgc acgggccaac ttttatgggc aatccgctgg cctgcgcggc tgcaaacgcc
    12721 agcctggcga ttctcgaatc tggcgactgg cagcaacagg tggcggctat tgaagtacag
    12781 ctgcgcgagc aacttgcccc cgcccgtgat gccgaaatgg ttgccgatgt gcgcgtgctg
    12841 ggggcaatcg gcgtagtcga aacaactcgt ccggtgaata tggcggcgct gcaaaaattc
    12901 tttgtcgaac agggggtatg gatccggcct tttggcaaac tgatttacct gatgccgccc
    12961 tatattattc tcccgcaaca gttgcagcgt ctgaccgcag cggttaaccg tgcggtgcag
    13021 gatgaaacat ttttttgcca ataacgggaa gtcagcgtgt gggtttctgg ctacactttc
    13081 tgcaaactag aaaggagggt tcatgaaact catcagtaat gatctgcgcg atggcgataa
    13141 gctgccgcat cgtcatgtct ttaacggcat gggttacgat ggcgataata tttcaccgca
    13201 tctggcgtgg gatgatgttc ctgcgggaac gaaaagtttt gttgtcacct gctacgaccc
    13261 ggatgcgcca accggctccg gctggtggca ctgggtagtt gttaatttac ccgctgatac
    13321 ccgcgtatta ccgcaagggt ttggctctgg tctggtagca atgccagacg gcgttttgca
    13381 gacgcgtacc gattttggta aaaccggata cgatggcgca gcgccgccga aaggcgaaac
    13441 ccatcgctac atttttaccg ttcacgcgct ggatgtagag catattgatg tcgatgaagg
    13501 agccagcggc gcgatggtcg ggtttaacgt tcatttccac tctctggcaa gcgcatcgat
    13561 taccgcgatg tttagttaat cactctgcca gatggcgcaa tgctatctgg tatcacttaa
    13621 aggtattaaa aacaactttt tgtcttttta ccttcccgtt tcgctcaagt tagtataaaa
    13681 aagcaggctt caacggattc atttttctat ttcatagccc ggagcaacct gtgaacacat
    13741 tttcagtttc ccgtctggcg ctggcattgg cttttagcgt gacgctgacc gcctgtagct
    13801 caacaccacc cgatcaacgt ccttctgatc aaactgcgcc tggtacctct tctcgcccga
    13861 ttctgtcggc aaaagaagcg cagaatttcg atgctcaaca ctattttgca tccctgacac
    13921 caggtgctgc agcgtggaat ccttccccga ttaccctgcc tgcgcaacct gactttgttg
    13981 tcggcccggc gggcactcaa ggtgtaacgc ataccacgat tcaggcggcg gtagatgcgg
    14041 caattatcaa gcgtaccaac aagcgccagt atattgccgt gatgcctggt gagtatcagg
    14101 gaacggtgta tgtccctgct gctccgggtg gaattactct gtacggtaca ggtgaaaaac
    14161 cgattgatgt gaagattggg ctttcccttg atgggggcat gagccctgcc gactggcgtc
    14221 acgacgtcaa cccgcgcggc aaatatatgc caggtaaacc agcgtggtat atgtacgata
    14281 gctgccagag caaacgcagc gacagtatcg gtgttctctg ctctgcggtc ttctggtcac
    14341 aaaacaatgg cctgcaactg caaaacctga ccatcgaaaa cacgctgggc gatagcgtag
    14401 atgcaggtaa ccatccggcg gtggcactgc gtactgatgg tgaccaggta cagattaaca
    14461 acgttaacat tctcggtcgt cagaacacct tctttgtcac caacagcggt gtgcagaacc
    14521 gtctggaaac gaatcgtcag ccgcgtacgc tggtgaccaa cagctacatt gaaggggatg
    14581 tggatatcgt ttctggtcgc ggcgcagtgg tgttcgataa caccgaattc cgcgtggtga
    14641 actcacgtac tcagcaagaa gcgtatgtgt ttgcaccggc tacgctgtcc aacatttact
    14701 acggtttcct cgccgtaaac agccgtttca atgctttcgg tgatggtgtg gcgcaactgg
    14761 gccgctcgct ggatgttgat gccaatacca acggtcaggt ggtgatccgt gatagcgcca
    14821 tcaacgaagg ttttaacacg gctaaaccgt gggccgatgc ggtgatctct aatcgtccgt
    14881 ttgcgggtaa taccggcagc gtagatgata acgacgaaat acagcgcaat ctgaatgaca
    14941 ctaactacaa ccgcatgtgg gaatacaata accgcggcgt gggtagtaaa gtggttgcag
    15001 aggcgaagaa gtaagagcaa ttaactattt gccggatgcg gcgtaaacgc cttatccggc
    15061 ctacggttcg atgcgatttg taggtcggat aagatgcgca agcatcgcat ccgacaataa
    15121 gtgccgaatg cgacctacat tcacatggcg ctttttacat ctgacggttt ttattgaagt
    15181 taatcaaact acccgccttg ataatctcgc gctcttcagc agtcagactt tccatataga
    15241 gcgtaatttc cgttaccggc gcatcttcat ggatcacata acctttaaac gtcgtacccg
    15301 gattatccag cgccgcttta atgccaggga tgtaaatgta atcccccact tcaaaggttg
    15361 gcacttccgc catctgcagc ggtaacatcc cccagttgat gacgttagaa cggtagcgtt
    15421 tagtcgcgta ctcttcggca atattcgcca gaccgccaat cacacgctgg cagctcgccg
    15481 cctgttcacg cgcagaacca tcgcctggtt tcaccgcata taccatgctg ccaatttcag
    15541 tttgcagcgg atcaatatgc tcctgaccag caatctgctt aatgcgcgca aacacctctg
    15601 tcagctcgct gacattcccc gccagacgct gattttccag ctcagcagtc gctttacttc
    15661 tgccaacata accgggatcg cggcgtgaca gggtaaactc cgccagacca atcggatttg
    15721 aacgataaga agaggtttca ccggaaggaa tcagttcgtc ggtggtggtc acttcgtcga
    15781 ggatcttcga gcacactttc aggacgatat tgtcagtcag cgcacccaat tccggccagt
    15841 ctttaatgtt cggtccgtaa atcagcggtt gctgagttgc ccctttcaca aagccctgat
    15901 aaacacggtt tttatacggc gttacatcga aggcgtactc cggcacgttg tcccagcaat
    15961 caagttcgct ggcagaggtt aaatagccac cgtttgccgc agtcgcagcg atagaacgag
    16021 cgtccatcaa cgccaccgct gacatctgcc cattagctgg cttagagcct tcgcggttcg
    16081 ggaagttacg cgtggtgtgg cgaatactta atccgttgtt gattggcgta tcgcccgcgc
    16141 caaagcatgg gccgcagaac gcggttctga tgattgcgcc tgcgccaatc aaatctgcta
    16201 ccacaccttt tttggctaaa tccataaaca ccggctgtga tgacgggtaa accgccagcg
    16261 agaaggtgtc attgccacag gattgaccgc gcagtgcatt cgccgccgcg atgacgtttt
    16321 cgtagttacc gccagaacag cccgcgataa tcccctgctg cactttcagg cgaccatttt
    16381 ccactttatc cagcagcgag agtttggctt taccgtgcgc cacgcgttcg gactcaattt
    16441 caatctcacg cagaatgtcg gtcaggttct ggttcagtgt gtcgatttca tacacgttgc
    16501 tcgggtggaa cggcagcgca atcattggtt tgatggcgct taaatcaacg ctgatgcagc
    16561 catcgtagta cgccatcggt tgagggttaa gctggcagta atcctggccg cgaccgtgca
    16621 gcgccagcca gttatgcact tcttcatcag tttgccagac ggagcttaaa caggtggttt
    16681 ccgtggtcat cacgtcaacg ctgttacgga aatcggtaga gagcgcgcta acgcccggtc
    16741 caacgaactc catgactttg tttttgacgt aaccgttttt gaacaccgcg ccaatgatag
    16801 ccagcgccac atcctgtggc cccacatacg gcgcaggttt tccggtcaga tgcaccgcaa
    16861 ccacgcccgg atagtcgata tcccaggtgt cattaagcag ctgttttacc aactcaccgc
    16921 cgccctcacc gactgccatt gtccctaatg caccgtaacg ggtgtggctg tctgacccga
    16981 ggatcatttt gcccccgcct gccatcatct cacgcatata ttgatggatg atcgcaatat
    17041 gcggaggcac aaaaataccg ccataacgct gagctgccga taaaccaaaa acatggtcat
    17101 caccgttaat ggtgccgccg acggcgcaga gtgagttatg gcagttggtc agcacatacg
    17161 gcagcgggaa acgttccatt ccggacgctt tagcggtctg tacaatacca acaaaggtaa
    17221 tatcgtgaga ggctaatgaa tcaaacttaa ttttaagttt atccatattt ccggacgtat
    17281 tatgagaaga gagaatagac caggcaatag tgcctttttt ggcttcttct tttttaattt
    17341 cgccggtgaa atgttcttcg gcaattattt cgttattact ggcgagaaac acgcctttt
//
LOCUS       DAJVUJ010000086        17365 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.086, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000086 DAJVUJ010000000
VERSION     DAJVUJ010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17365
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.086"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(254..535)
                     /locus_tag="OQQ48_002536"
     CDS             complement(254..535)
                     /locus_tag="OQQ48_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HCS1953267.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLAINGRISPPTCSMAMV
                     NGQPQQHCGQLTYNVDTRHLFSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            complement(798..1907)
                     /gene="apbC"
                     /locus_tag="OQQ48_002537"
     CDS             complement(798..1907)
                     /gene="apbC"
                     /locus_tag="OQQ48_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HCS1953268.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            2039..4072
                     /gene="metG"
                     /locus_tag="OQQ48_002538"
     CDS             2039..4072
                     /gene="metG"
                     /locus_tag="OQQ48_002538"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCS1953269.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPSNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMRQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            4213..8007
                     /locus_tag="OQQ48_002539"
                     /pseudo
     CDS             4213..8007
                     /locus_tag="OQQ48_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR and DUF4132 domain-containing protein"
     gene            8017..11649
                     /locus_tag="OQQ48_002540"
     CDS             8017..11649
                     /locus_tag="OQQ48_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HCS1953270.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRRWPGEPVSVITGSLIQ
                     TLGDELLQKAEKKKNIVWRYENFSLEWQSAITQAINLIGEHKPSIPARTMAALACIAQ
                     NDSQQLLDEIVQQEGLEYATEVVIARQFIARCYESDPLVVTLQYQDEDYGYGYRSETY
                     NEFDLRLRKHLSLAEESCWQRCADKLIAALPGITKVRRPFIALILPEKPEIANELVGL
                     ECPRTHFHSKEWLKVVANDPTAVRKLEHYWSQDIFSDREASYMSHENHFGYAACAALL
                     REQGLAAIPRLAMYAHKEDCGSLLVQINHPQVIRTLLLVADKNKPSLQRVAKYHKNFP
                     HATLAALAELLALKEPPARPGNPIIEDKKLPAQQKARDEYWRTLLQTLMASQPQLAEE
                     VMQWLSTQPQSVLKSYLSAPPKPVIDGTDNSNLPEILVSPPWRSKKKMTAPRLDLAPL
                     ELTPQVYWQPGEQERLAATEPARYFSTESLAQRMEQKSGRVVLQELGFGDDVWLFLNY
                     ILPGKLDAARNSLIVQWHYYQGRVEEILNGWNSPEAQLAEQALRSGHIEALINIWEND
                     NYSRYRPEKSVWNLYLLAQLPREMALTFWLRINEKKHLFAGEDYFLSILGLDALPGLL
                     LAFSHRPKETFPLILNFGATELALPVARVWHRFAGQRNLARQWILQWPEHTATALIPL
                     VFVKPCDNSEAALFALRLLYEQGHSELLQTVANRWDRADMWPALEKILTQNPMEIYPA
                     RIPKAPDFWHPQMWSRPRLITNNQTVTNDALEIIGEMLRFTQGGRFYSGLEQLKTFCQ
                     PQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFGDESTARDLTTQILAWPQEGKSAR
                     AVSGLNILTLMNNDMALIQLHHISQRAKSRPLRDNAAEFLQVVAENRGLSQEELADRL
                     VPTLGLDDPQALSFDFGPRQFTVRFDENLNPVIFDQQNVRQKSVPRLRADDDQLKAPE
                     ALARLKGLKKDATQVSKNLLPRLETALRTTRRWSLADFHSLFVNHPFTRLVTQRLIWG
                     VYPANEPRCLLKAFRVAAEGEFCNAQDEPIDLPADALIGIAHPLEMTAEMRSEFAQLF
                     ADYEIMPPFRQLSRRTVLLTPDESTSNSLTRWEGKSATVGQLMGMRYKGWESGYEDAF
                     VYNLGEYRLVLKFSPGFNHYNVDSKALMSFRSLRVYRDNKSVTFAELDVFDLSEALSA
                     PDVIFH"
     gene            11710..12027
                     /locus_tag="OQQ48_002541"
     CDS             11710..12027
                     /locus_tag="OQQ48_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1953271.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDADPLINDALIYIRDECIDERKLRGESPETVIAIFNRELQNIFNQ
                     EIE"
     gene            12334..13422
                     /locus_tag="OQQ48_002542"
     CDS             12334..13422
                     /locus_tag="OQQ48_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HCS1953272.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDEAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATAISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVECIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            13433..15712
                     /locus_tag="OQQ48_002543"
     CDS             13433..15712
                     /locus_tag="OQQ48_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1953273.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQAARRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQDESQALLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDASGDALNRQREAFMARWIGWAMQQNNGDVLVVCGGWHAPALAKMWRECPQKIN
                     KPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQKAGEQLLKT
                     ILTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFTPDGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLQEAARNKLEADMLDAGGIGSITTCLSQAALAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCATIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVNLPGVSLSAAVALLERRSQAIHAPALDRGAALGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLS
                     EADFINALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQNWGVFHV"
     gene            15705..16841
                     /locus_tag="OQQ48_002544"
     CDS             15705..16841
                     /locus_tag="OQQ48_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCS1953274.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVCQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIPSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            16838..>17365
                     /locus_tag="OQQ48_002545"
     CDS             16838..>17365
                     /locus_tag="OQQ48_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HCS1953275.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     GALIATFSDGVRTQLANGQALKEAQCSCGASGMCRHRVMLVLSYQRLCATAQPTGKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKGITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEH"
BASE COUNT         4393 a         4474 c         4392 g         4106 t
ORIGIN      
        1 gtctgtatct cttggaaaca aatttctcac atcaaaaaag ttgccgcaat gtaaacacat
       61 tgttttattg atttaaaaca aattaaccat tgcaatataa atacaaaaaa tatttaagag
      121 tattaactat ttatcgcatc tatcaattaa tgtagattta tgtaaatggt attgttgata
      181 tcaataaaaa agccgaaatc atttatataa atgatttcgg ctttctaaat gcaattaaaa
      241 tcaggttgcc aaattaatcg tagcgattta acacgattcg acgtttgcta tcgctacctg
      301 caacaaccac ttctgtcgtt acacctttga cgggcgaaga aaacagatga cgggtgtcaa
      361 cattatacgt taactgacca caatgctgtt gaggttgacc attaaccata gccatactgc
      421 atgttggggg actgatcctg ccattaatcg ctaatgtggc agtctctgaa gagaatgcgg
      481 gagaagccaa tccataggca agaaaaataa ttcctgacag ccaatactta ttcattgaac
      541 gttatccctg tagtaaaggt tatgcctgga tagaatgagt gcataacaaa ctatagctgt
      601 acatccacta cacagccacg aaggatgata atgaagcatt gcctgtatga tcaatcgact
      661 ttgtagagtt tcggacgaag gtccgcagaa tattcgcagt attaaataag tgttcattaa
      721 tgcggcaaaa actaatacac cgcatcaatg taacatctct gcggtgtatg aagaataacc
      781 agaatggtta ataggcgtta gaccgcgcgg aaggaaatct cgcctggaat gacttcaccc
      841 tgccagtaga gctgagctgc aacgcggtca gccagctggc ggtagatagc ggtaaattcg
      901 ctctccggac ggctgataac cgttggcgta cctttatcga gatcctcacg taaggagatg
      961 tgtaacggca tctgccccag cagctgtgta tggtattttt cagccagttt ctctgcacca
     1021 ccggtaccga aaatcggttc atggtgacca cagttactgc aaatatgcac actcatgttc
     1081 tcgacaatac cgagcaccgg aacctcgact ttctcgaaca tcacaatgcc ttttttcgca
     1141 tcgatcagcg cgatgtcctg cggggtggtg accacgactg caccggttac tggaatgttt
     1201 tgcgccagcg tcaattgaat gtcaccggtg cctggcggca tatcaagcac cagatagtcg
     1261 agatccggcc acaaggtttc ctgcaacatc tgcattaacg ccttgctcgc catcggtcca
     1321 cgccacacca tcgcattgtc gtcagtgacc agataaccaa ttgaattggt tgccaggcca
     1381 tgagacataa tcggtgccat atgggtaccg tccggcgaag ttggacgctg attttctgcg
     1441 cccaacattg ttgggattga tgggccgtag atatcggcgt ccagaatgcc gactttcgcc
     1501 ccttctgccg ccagcgccag tgccaggttt accgccgtgg acgatttacc cacgccgcct
     1561 ttaccggaac tcaccgcgat aatgttcttc acaccgttaa ttcccggctg attcttcacg
     1621 cgtttcagag tggctatgtt atgcgacagt ttccagtcga tagctttcgc cccggtgatg
     1681 cgcagcagtt cggcgctaca ttgctctttt aactcttcga acgcactatg ccagacaaac
     1741 ggcataacca gttccacatg caacgtgtcg tccatccagg cgacatggtg caacgctttc
     1801 agcgtggtga ggttatgctt cagggttggg tgctgaaaat tggccagtgt cccggcgacc
     1861 attgcgcgca aggcttctgg tgatttggcc tgggattgtt cgttcatccc gactcctttt
     1921 ttgtatagat aaaccatcag ttgatagttt acctgaagaa tatagagaag tacttactta
     1981 acattttccc atttggtact atctaacccc ttttcactat taagaagtaa tgcctactat
     2041 gactcaagtc gcgaagaaaa ttctggtgac gtgcgcactg ccgtacgcta acggctcaat
     2101 ccacctcggc catatgctgg agcacatcca ggctgatgtc tgggtccgtt accagcgaat
     2161 gcgcggccac gaggtcaact tcatctgcgc cgacgatgcc cacggtacac cgatcatgct
     2221 gaaagctcag cagcttggta tcaccccgga gcagatgatt ggcgaaatga gtcaggagca
     2281 tcagactgat ttcgcaggct ttaacatcag ctatgacaac tatcactcga cgcacagcga
     2341 agagaaccgc cagttgtcag aacttatcta ctctcgcctg aaagaaaacg gttttattaa
     2401 aaaccgcacc atctctcagc tgtacgatcc ggaaaaaggc atgttcctgc cggaccgttt
     2461 tgtgaaaggc acctgcccga aatgtaaatc cccggatcaa tacggcgata actgcgaagt
     2521 ctgcggcgcg acctacagcc cgactgaact gatcgagccg aaatcggtgg tttctggcgc
     2581 tacgccggta atgcgtgatt ctgaacactt cttctttgat ctgccctctt tcagcgaaat
     2641 gttgcaggca tggacccgca gcggtgcgtt gcaggagcag gtggcaaata aaatgcagga
     2701 gtggtttgaa tctggcctgc aacagtggga tatctcccgc gacgcccctt acttcggttt
     2761 tgaaattccg aacgcgccgg gcaaatattt ctacgtctgg ctggacgcac cgattggcta
     2821 catgggttct ttcaagaatc tgtgcgacaa gcgcggcgac agcgtaagct tcgatgaata
     2881 ctggaagaaa gactccaccg ccgagctgta ccacttcatc ggtaaagata ttgtttactt
     2941 ccacagcctg ttctggcctg ccatgctgga aggcagcaac ttccgcaagc cgtccaacct
     3001 gtttgttcat ggctatgtga cggtgaacgg cgcaaagatg tccaagtctc gcggcacctt
     3061 tattaaagcc agcacctggc tgaatcattt tgacgcagac agcctgcgtt actactacac
     3121 tgcgaaactc tcttcgcgca ttgatgatat cgatctcaac ctggaagatt tcgttcagcg
     3181 tgtgaatgcc gatatcgtta acaaagtggt taacctggcc tcccgtaatg cgggctttat
     3241 caacaagcgt tttgacggcg tgctggcaag cgaactggct gacccgcagt tgtacaaaac
     3301 cttcactgat gccgctgaag tgattggtga agcgtgggaa agccgtgaat ttggtaaagc
     3361 cgtgcgcgaa atcatggcgc tggctgatct ggctaaccgc tatgtcgatg aacaggctcc
     3421 gtgggtggtg gcgaaacagg aaggccgcga tgccgacctg caggcaattt gctcaatggg
     3481 catcaacctg ttccgcgtgc tgatgactta cctgaagccg gtactgccga aactgaccga
     3541 gcgtgcagaa gcattcctca atacggaact gacctgggat ggtatccagc aaccgctgct
     3601 gggccacaaa gtgaatccgt tcaaggcgct gtataaccgc atcgatatga ggcaggttga
     3661 agcactggtg gaagcctcta aagaagaagt aaaagccgct gccgcgccgg taactggccc
     3721 gctggcagat gatccgattc aggaaaccat cacctttgac gacttcgcta aagttgacct
     3781 gcgcgtggcg ctgattgaaa acgcagagtt tgttgaaggt tctgacaaac tgctgcgcct
     3841 gacgctggat ctcggcggtg aaaaacgcaa tgtcttctcc ggtattcgtt ctgcttaccc
     3901 ggatccgcag gcactgattg gtcgtcacac cattatggtg gctaacctgg caccacgtaa
     3961 aatgcgcttc ggtatctctg aaggcatggt gatggctgcc ggtcctggcg ggaaagatat
     4021 tttcctgcta agcccggatg ccggtgctaa accgggtcat caggtgaaat aatccccctt
     4081 caaggcgctg catcgacagc gccttttctt tataaattcc taaagttgtt ttcttgcgat
     4141 tttgtctctc tctaacccgc ataaatactg gtagcatctg cattcaactg gataaaatta
     4201 cagggatgca gaatgagaca ctttatctat caggacgaaa aatcacataa attcagggca
     4261 gttgagcaac agggaaacga gttgcatatc agttggggaa aagttggcac caaagggcaa
     4321 agccagataa aaagtttttc agatgctgcg gcagcggcaa aagcggagct taagctgatt
     4381 gcggagaagg tgaagaaggg gtatgtggag caagcgaagg ataattcttt gcaaccttcc
     4441 caaacggtaa cgggctctct caaggtagcg gacttatcca ccattattca ggaacaaccc
     4501 tctttcgtag cagaaacccg tgcgcctgac aaaaatacag atgctgtttt accgtggctg
     4561 gcgaaagata ttgctgtcgt ttttccgccc gaagttgtac acaccacgtt aagtcatcgc
     4621 cgctttcccg gagttcctgt tcagcaagca gacaaattgc cccaattacg tcgcttagcc
     4681 tgtagtgtgt cgcaacggga taataaaaca gccacatttg acttcagcgc ctgttcttta
     4741 gaatggcaaa acaccgtcgc ccaggcgatc agtcagatcg acggcctgaa aacaacacag
     4801 ttaccatcac cagtaatggc tgtactcacg gcacttgaaa tgaaatgcac aagatataaa
     4861 gtgcgtgagg atgttatgga tcagatcgtc caggaaggcg gtctggaata tgctactgat
     4921 gtaataatac accttcaaca gattgatatt gaatgggatt atgcgaataa tgtcattatt
     4981 attctgccgt ctggcattgc acctagctac ttggagcaat attccagatt tgaatgacgc
     5041 ctacgtaaac atttatcact gacggaagag tctctctggc aaaaatgtgc acaaaaactt
     5101 attgccgcaa ttccacatat tccagaatgg cggcaaccat taattgcttt gttattaccc
     5161 gaaaaaccag aaattgcaca tgaaattgcc cagcgtctac tggggcaaaa aaaattaccc
     5221 tcgcttgagt ggttaaaaat agtggcgact gatgagcaca ttcttgcctc attagaaaaa
     5281 tatcacgaac catatgccat ttttgatgat tactattgtg gtgcgatatg gtcagccacc
     5341 gtattacagg agcaaggtgt tgcagccctg ccccgatttg ctccctatgc cgcaagtgac
     5401 tactgcgccg atgtgttgcg tcatatcaat catccgttcg cattgacact gcttatacgt
     5461 gtagccgggc aaactaaacg ctgtcacgat cggatgacga aagccattgc tgcgttccca
     5521 catgcagcaa tggcggcact gacggaactt cttgggcaaa aagaagagaa cagttggcgc
     5581 attatgctaa tgacaatgct tatctcacaa ccagcactgg cagaacaggt cattccctgg
     5641 ctctcgacac ccgcagttgc cgtactgaaa tcatgccagc aacaactgac acagccctca
     5701 aaccatgcca gcgccgatct actgccagcc gtagtagtct cccctccctg gctttcgaaa
     5761 aagaaaaaat cgccgattcc ggtgctggat ttagcgccat taggcattga gccaatctgt
     5821 tatctgacag aagaaatcag taatcaactt ttggcgaaat atatctggta ttcaaaacac
     5881 atcacggtta gccatgaaga aagtactacc aacctgttgg caaggatggg ttttcaacga
     5941 cggatcgctg gtacatatat taaagctccc gaagcggtag ttgaggcatg gctaaatgaa
     6001 gattattcaa ccttactaag tgaatttaag gtgtttcatt cacctaccgg gcattattgg
     6061 cagttgggga ttttgacaac attgccgctg gagaaagcag taaaagcatg gaatgccctt
     6121 accctatctc cacataccga taccgaatac tccatgttac attttggact caaagggtta
     6181 cctgggttag taaactcact tgcacgctat ccacaagaag ccttgcccat cacgaattac
     6241 ttcgcagcga gtgagctggc tcctgccgtc gcccgtgcct tcaacaaact gaaaacgcta
     6301 cgcgaaaacg cccgtagctg gctgttgaaa tacccggaac atgcccttac cggcctgctg
     6361 cctgcggcgc tcggcaaagc cggtgaagca caggataacg cccgcgctgc cttgcgtatg
     6421 cttaccgaaa acggtcatca gccattactg caagaaatcg cccgacgtta taaccagccg
     6481 gaagtaaccg atgcggtgaa cgctctgctt gcgctcgatc ccttagataa tcacccgaca
     6541 aaaatcccca ctcttccggc cttttatcag ccatcgctct ggacgcgccc ggtattaaaa
     6601 gcaaatgccc aatcactgcc agatagcgcc ctcctccacc tcggtgaaat gctccgcttc
     6661 cctcaggaag aggctctgta tccgggatta ttgcaggtga aagacgtctg ttccgccgac
     6721 tcactggcgg gatttgccta ggatctgttt accgcctggc agaccgctgg cgcgccgtcg
     6781 aaagagagtt gggcgttcac tgcgttaggc gttctcggta acgatgacac cgcccgcaaa
     6841 ctgacgccat taatacgcgc ctggcctggt gaatcccagc ataaacgcgc caccgttggg
     6901 ttggatattc tcgctgctat cggtagtgat atcgccctta tgcagcttaa cggcatcgcc
     6961 cagaaactga aattcaaagc attacaggag cgggcaaaag aaaaaattgc cgacattgcc
     7021 gagagccgcg aactcacggt ggcggagctt gaagatcggt tagcaccgga tctcggtctg
     7081 gatgataacg gttcgctgct gctggatttt ggcccacggc agttcaccgt cagctttgat
     7141 gaaaccttaa aaccgtttgt gcgtgatgtt tccggcagcc gcctgaaaga cctgcccaaa
     7201 ccgaacaaaa gcgatgatga aacgcgggcg aacgatgcgg ttaaccgcta caaattgctg
     7261 aaaaaagatg cgcgtaccat cgccgcccag caggtagcaa ggctggaatc cgccatgtgc
     7321 ctgcgccgcc gctggtcgct ggaaaacttc cagctcttcc tggttgagca tccgctggtt
     7381 cgtcacttaa cccgccgtct gatttggggc gtttatagcg ccgaaaacca gctactggct
     7441 tgctttcgcg tagcagaaga taacagctcc agcaccgctg acgatgatct tttcaccctg
     7501 ccggaaggcg atatctctat cggcactcct cacgttctgg aaatatcacc aacggatgct
     7561 gccgcctttg gtcagctttt tgccgactac gaactgctac caccgttccg ccagctcgac
     7621 cgtaacagct acgccctgac agaagccgag cgcaatgcca gtgaactgac ccgctgggca
     7681 ggcagaaaat gcccgagtgg tcgggtcatg gggctggcga ataaaggctg gataaagggc
     7741 gaaccacagg atggaggctg gatcggatgg atgatcaaac ctttgggtcg ctggtcgtta
     7801 atcatggaaa tcgatgaagg ctttgcggta ggcatgtcgc cagccgaact cagcgctgag
     7861 cagctcttaa gcaagctgtg gctatgggaa ggcaaagcag aaagatatgg ctgggggagt
     7921 aattcaacac aggaagcgca gttctccgta atcgatgcca tcaccgccag cgagctaatt
     7981 aacgatattg aagccctgtt tgaataagga aagagcatgg acaaggaatt accgtggctg
     8041 gcggataacg cccaactgga actgaaatat aaaaaaggca aaacgccgct cagtcatcgt
     8101 cgctggccgg gcgaaccagt gtccgttatc actggaagtc tcatccagac attgggtgat
     8161 gaattgctac aaaaagctga gaagaaaaaa aacattgtct ggcgttatga gaatttttca
     8221 ctggagtggc agtccgccat cacgcaggcc atcaacttga tcggcgaaca caaaccctca
     8281 atcccggccc ggacaatggc ggcgctagcc tgtatcgcgc aaaatgacag ccaacagttg
     8341 ctcgacgaaa tcgtccaaca agaggggctg gaatatgcga ctgaggtggt gattgcacgc
     8401 cagtttattg cgcggtgtta tgagagtgat cctctggtag tgacattgca gtatcaggac
     8461 gaggattatg gctatggtta tcgctcagaa acctataacg aattcgatct ccgactgcgt
     8521 aagcatctct ctctggcaga ggaaagctgc tggcagcgtt gcgccgacaa actcattgcc
     8581 gcactaccag gaataaccaa agttcgccgc ccttttattg cgctgatcct cccggaaaaa
     8641 ccagaaatag ccaatgagtt ggtaggcctt gaatgcccgc gaactcattt tcattctaag
     8701 gagtggttaa aagttgttgc taatgacccc acagcggtga gaaaactcga acactactgg
     8761 agccaggata tatttagcga tcgagaagcc agctacatgt cgcatgaaaa ccacttcggc
     8821 tacgcggcct gcgccgccct tttgcgcgaa caaggactgg cagccattcc gcgcctcgcg
     8881 atgtatgccc ataaagaaga ttgcggcagt ctgctggtac aaattaacca tccgcaagtc
     8941 atccgcacct tgctactggt ggctgataaa aacaaaccca gcctgcaacg tgtagctaaa
     9001 taccataaaa acttccccca tgcgacgctc gccgcactgg cagaactgct ggcgttaaaa
     9061 gaaccaccag cccgcccagg taatccaatc atcgaagaca aaaagctgcc tgcacagcaa
     9121 aaagcacgcg atgaatactg gcgtacgctg ttacaaacgc tgatggcatc gcagccacaa
     9181 ctggcagaag aggtgatgca gtggttaagt actcaacccc agtcagtgct gaagagttat
     9241 ttatcggcac cgcccaaacc ggttattgat ggcaccgata acagcaatct gccagaaatc
     9301 ctcgtttcac caccgtggcg tagtaagaaa aaaatgacag ctccacgtct tgatttggca
     9361 ccgctcgaat taactccgca agtttactgg caaccaggcg aacaagagag gcttgccgcc
     9421 actgagcctg cccgttattt cagcacggaa tctcttgcgc aacgcatgga acaaaaaagt
     9481 ggacgagttg tattacagga actgggtttt ggggatgatg tatggctgtt tctgaattat
     9541 atactccccg gaaaactgga tgctgcacgc aattcactca ttgttcagtg gcattactac
     9601 caggggcggg ttgaagagat cctgaatggc tggaactccc cggaagcaca attagcagaa
     9661 caggcgctcc gcagcggtca catagaagcg ttaattaaca tatgggaaaa tgacaactac
     9721 tcacgttatc gtccggaaaa gagtgtctgg aacctgtatt tattggcaca gttgccgcgt
     9781 gagatggctt tgaccttctg gctgcgtatc aatgagaaaa agcatctgtt cgcgggtgag
     9841 gactattttc tcagtatcct cggattggat gcgctaccag gtctgctgtt ggctttttca
     9901 catcgtccaa aagaaacatt tccgttaatt ttaaatttcg gcgcaacaga actggccctg
     9961 cccgttgccc gcgtctggca ccgttttgcg ggccagcgta atctggctcg ccagtggatt
    10021 ttacaatggc cggaacatac ggctactgca cttattccac tcgtctttgt taaaccctgc
    10081 gacaacagcg aagcggcatt atttgccctt cgtttactgt atgaacaagg acatagtgaa
    10141 ttactgcaaa cggttgcaaa ccgctgggat cgcgctgata tgtggccagc cctggaaaaa
    10201 atacttaccc agaacccgat ggaaatttac ccggcacgca ttccaaaagc ccctgatttc
    10261 tggcatccgc aaatgtggtc caggccgcgc cttatcacta ataatcaaac tgttaccaat
    10321 gacgctctgg aaattatcgg cgaaatgctg cgctttaccc aggggggacg tttttatagc
    10381 gggctggaac aactgaaaac gttctgccag ccacaaacgc tggcagcttt tgcttgggat
    10441 ctcttcactg cgtggcaaca agctggtgcc cccgcaaaag acaactgggc atttctggcg
    10501 ttaagtctct ttggtgacga aagcacggca cgggatctga cgacacagat cctcgcctgg
    10561 ccacaagaag gcaaatctgc ccgtgctgtc agcggcctga acatccttac cctgatgaat
    10621 aatgatatgg cgctgataca gctgcatcat atatcgcaac gggctaaatc ccgcccctta
    10681 cgtgataacg cggcggaatt tcttcaggtg gtcgcagaaa atcgcgggct aagccaggaa
    10741 gagctagcgg acagattagt cccaaccctg ggccttgatg atccgcaggc gttgagtttt
    10801 gattttggtc cccggcagtt taccgttcgc ttcgatgaaa atctcaaccc ggttatcttt
    10861 gatcagcaaa acgttcgcca gaaaagcgtt ccccgtttgc gcgccgatga cgatcaactg
    10921 aaagcgcccg aggcactggc ccgactaaaa gggctaaaaa aagatgctac tcaggtgagc
    10981 aaaaacctgc tcccgcgtct tgaaacggcc ctacgtacca cccgacgctg gtcgctggca
    11041 gattttcatt ctctgtttgt taatcatccc tttacccgtc tggttaccca gcgattaata
    11101 tggggggttt atccggcaaa tgaaccgcgt tgtttactca aagcctttcg tgtggccgca
    11161 gagggggagt tctgcaatgc gcaagatgag ccaattgacc tgcctgcgga cgctctgatt
    11221 ggcattgccc acccgttaga aatgacagca gaaatgcgca gtgaatttgc acagcttttt
    11281 gccgattacg aaattatgcc gccttttcgc cagttgtcgc gccgcacggt gctgctcaca
    11341 cctgacgagt caaccagtaa cagcctgact cgctgggaag gtaaatccgc taccgttggg
    11401 caacttatgg gaatgcgata caaaggctgg gagtcaggct atgaggacgc atttgtctat
    11461 aacctgggtg agtaccggct ggtccttaag ttttcacccg gttttaacca ctacaatgtt
    11521 gatagcaaag cgctaatgag cttccgttct cttcgagtgt accgtgacaa taaatccgtc
    11581 acttttgccg aacttgatgt gtttgatttg agtgaggcgt taagcgcacc tgacgtcatt
    11641 ttccattaaa acacagcggg cagtgatgca actgcccgtt atcaacacga cttaccagcg
    11701 gggaaagcga tgatcgtgca aaaagagctg gttgctattt acgattatga ggtccctgta
    11761 cctgaagatc cgttttcctt cagacttgag atccataaat gctctgaatt atttacaggt
    11821 tccgtctatc gactggagcg attccggcta cgtccaacat ttcatcaacg tgatcgagaa
    11881 gatgctgacc cgctaataaa tgatgcgttg atttatataa gagatgagtg tattgatgag
    11941 cggaaattac gaggtgaatc acctgaaact gtaatagcaa tttttaatcg tgaactacag
    12001 aatatattca accaagaaat agaataatat actctaaata attcaaattg gtccgatccg
    12061 gcgcaacgtc ccaatggcct ggattataaa tctcattatc ttaattgcaa cggggtccag
    12121 ccgtggttat aattcgttcg ttggaacacg atgttgcaca ggctgtggtg ttaggcctga
    12181 aaatagtaaa taaaagaacc tgcctcacca gcaggttttt ttatttactg tgatctgctt
    12241 tccagatatt tttcgctcaa acaactaatg cgccaaacat ttattgcgcg taaaatatcg
    12301 tttatttcat taatacattt cagggatgaa tatatgtcac cgcagaataa tcatctgcag
    12361 cgtccgcctg ctgctgtgtt atacgccgat gaactggcaa aattaaaaca aaatgataac
    12421 gcaccttgcc cgcccggttg gcagttaagt ttgcctgcgg cccgtgcttt tatccttggc
    12481 gacgaagcgc aaaatatcag ccgtaaagtg gtgattagcc cctccgctgt cgaacgtatg
    12541 ttagtcactc ttgctaccgg ccgcggtttg atgttggtgg gggaaccggg taccgcaaaa
    12601 tctcttcttt ctgaattact ggcaaccgct attagcggcg atgccgggtt aaccatccag
    12661 ggcggggcat ctaccaccga agatcaaatc aagtatggct ggaactacgc cctgcttatc
    12721 aatcatggtc cgtcaacgga agcgctggtc cctgccccgc tttatcaggg aatgcgcgac
    12781 ggcaaaatcg tccgtttcga agagataacg cgtacaccgc tggaagtgca ggactgtctg
    12841 ttaggtatgc tttctgacag ggtgatgacg gtgccggaac tcactggtga agccagtcag
    12901 ctctatgcgc gagaaggctt taatattatt gccactgcca atacccgcga tcgcggcgtc
    12961 aatgaaatga gtgctgcgct aaagcgtcgc ttcgattttg aaacggtttt tccgattatg
    13021 gatttcgccc aggagttgga actggtcgcc agtgcttcgg cgcgtttgct ggcccatagc
    13081 ggtattccgc ataaagtgcc tgacgcggta ctggaattac tggtccgcac cttccgcgat
    13141 ctgcgtgcca acggcgaaaa gaaaacgtca atggatacac tgacagcgat tatgtccacc
    13201 gctgaagccg tgaacgtggc ccatgctgtg ggcgtccgcg cctggttttt agcgaatcgc
    13261 gcgggggagc ctgccgacct ggtggaatgt attgccggaa ccatcgtcaa agataacgaa
    13321 gaagatcgcg cgcgtctgcg ccgttacttc gaacagcgtg tcgccacaca taaagaagct
    13381 cactggcagg cttattatca agcccgccac cgcctgccgt gaggaaagat gcatgagcga
    13441 gccgttaatt gtcggcatcc ggcatcatag tccggcctgc gcccggctgg tgaaatcgtt
    13501 aatcgaaagc cagcggccac gatacgtgtt gattgaaggc ccggctgatt ttaatgaccg
    13561 ggtagacgaa ctgtttttag cccaccagct tccggtagct atttacagtt attgccagta
    13621 tcaggacggt gcagcccccg ggcgtggtgc ctggacgcca tttgctgaat tttcgccgga
    13681 gtggcaggcg ctacaagccg cacgtcgcat tcaggcacaa acttacttca tcgatttgcc
    13741 ttgctgggcg cagagtgaag aagaggacga ttcgcctgat acgcaagatg aaagccaggc
    13801 cttactgctg cgtgccaccc gcatggataa cagcgatacc ctgtgggatc acttgttcga
    13861 agatgaaagc cagcaaactg cattaccctc tgcgctggcg cactattttg cccaactgcg
    13921 gggcgacgcc tccggcgatg cgctcaatcg tcagcgcgaa gcctttatgg cccgctggat
    13981 tggatgggcg atgcagcaaa ataatggcga cgtgttagtt gtctgcggtg gctggcacgc
    14041 tccggcactg gcaaagatgt ggcgcgaatg ccctcagaaa attaacaagc cagaattgcc
    14101 ctcgctggca gatgccgtta caggttgtta tctcacaccc tacagtgaaa agcgccttga
    14161 tgtgctggca ggataccttt caggaatgcc tgccccggta tggcaaaact ggtgctggca
    14221 gtggggcttg cagaaggccg gtgaacaact gctaaaaact atccttaccc gtttgcgcca
    14281 gcacaaattg cccgcttcta ccgcggatat ggctgccgct catctgcatg cgatggcgct
    14341 ggcacagttg cgcggtcata cactaccgtt acgcactgac tggctggatg ccatagcagg
    14401 ctcgctgatt aaagaagccc tgaacgcgcc gttgccgtgg agctatcgcg gcgttattca
    14461 tcccgatacc gatccgattc tgctaacgtt gatagacaca ttagcgggtg acggattcgg
    14521 taaacttgcc ccttctacgc cacaaccgcc tctgccaaaa gatgtcacct gcgaactgga
    14581 acgtaccgca atctcccttc cggcggagct taccttaaat cgctttaccc ccgatgggct
    14641 ggcgcaaagt caggtgttac atcggctggc aatactggag atccctggga ttgtacgcca
    14701 gcagggaagt acactgacac ttgcaggcaa cggtgaagaa cgctggaaat taacccgccc
    14761 gcttagccag catgcggcat tgattgaggc cgcctgtttt ggtgccacac tccaggaagc
    14821 cgcacgcaat aaattagaag ccgatatgct ggacgcgggc ggaatcggca gtatcaccac
    14881 atgtcttagc caggcggcgt tagcgggtct ggcgtccttc agtcaacaat tactggagca
    14941 actcacacta ttaatcgccc aggaaaatca atttgccgaa atgggccagg cgctggaagt
    15001 gctttatgcc ttatggcggc tggatgaaat tagcggtatg caaggcgcgc agatattaca
    15061 aacgacgtta tgcgcgacta tcgatcgcac gctgtggctg tgtgaatcta acggcagacc
    15121 ggatgaaaag gagtttcacg ctcacctgca tagctggcaa gcgctttgcc atattctgcg
    15181 cgatctacat agcggcgtta atttacccgg cgtttctctt tctgcggcgg tagccttact
    15241 ggagcgacgc agtcaggcaa ttcatgcccc ggcgctggat cgcggcgcgg ctcttggcgc
    15301 actaatgcgt ctggaacatc ccaacgccag tgccgaagcg gcgctgacga tgctggcgca
    15361 gttatccccg gcacaatctg gtgaggcgct gcacggtttg ctggcgctgg cccgccatca
    15421 actggcctgt cagccggcat ttatcgccgg tttcagcagt catttaaatc aactgagtga
    15481 agccgatttt attaacgccc tgcccgattt acgcgcggcg atggcctggc taccaccacg
    15541 agaacgcggg acgctggcgc atcaggtgct tgagcattat caactggcgc aacttcccgt
    15601 ttcggcgctg caaatgccgt tgcattgtcc accacaggcc attgcacatc atcaacaact
    15661 cgaacagcag gcactggcat cgctgcaaaa ctggggagtt ttccatgtct gaactgaacg
    15721 atcttctgac cacccgtgag ctacaacgct ggcgattaat tcttggcgaa gcggcagaaa
    15781 cgacgctttg tgggctggat gacaacgccc ggcagataga ccacgcgctg gagtggctgt
    15841 atgggcgcga tcctgaacgg ctccagcgtg gtgaacgttc cggtggatta ggtggctcaa
    15901 atctcaccac ccctgagtgg atcaacagta ttcacacgct gtttccgcag caggtgattg
    15961 agcggctgga aagcgatgcc gtgctgcgct acggcattga agatgtggtg acgaatctcg
    16021 acgtgctgga acgtatgcag ccttctgaaa gcctgctacg cgctgttttg cacaccaaac
    16081 atctgatgaa ccccgaagta ctggctgccg cccgccggat agtgtgccag gttgttgaag
    16141 aaattatggc tcgactggca aaggaagttc gtcaggcttt ttctggtgtc cgcgatcgcc
    16201 gtcgccgttc atttattcca ctggcgcgaa actttgattt caaaagtact ctgcgcgcca
    16261 acctgcaaca ctggcacccg caacacggca agttgtatat cgaatccccc cgctttaaca
    16321 gccgcattaa acgccaaagc gaacaatggc aactggtctt actggttgat caaagcggct
    16381 cgatggtcga ttcggtgatc ccctctgcgg tgatggcggc ctgtttgtgg cagttacccg
    16441 gcattcgtac ccatctggtg gcgtttgaca caagcgtcgt tgatctcacg gcagacgttg
    16501 ccgatccggt agagttatta atgaaagtac agttgggcgg cgggaccaat atcgccagtg
    16561 ccgtggagta tggtcggcaa cttattgaac aaccagcgaa aagcgtcatt atcctcgtga
    16621 gcgattttta cgaagggggt tcatcatcat tactgacgca tcaggtgaaa aagtgtgtcc
    16681 agagcggcat caaagtgctg ggactggcag cgctcgatag cacagcaaca ccttgctatg
    16741 accgcgatac ggcccaggcg ctggttaatg tcggcgcaca aatagccgcc atgacaccgg
    16801 gcgagctggc atcatggctt gcggagaatc ttcagtcatg aattcactac gtccggaatt
    16861 attagaactg acaccgcagg ccctgacggc gttaagcaat gccggttttg ttaagcgcag
    16921 tcttaaggaa ctggaaaatg gcaacgtccc ggagatcagc catgagaacg gcgctttaat
    16981 cgccaccttc agtgacggtg tccgtaccca gctggcgaac ggccaggcac tgaaagaggc
    17041 tcagtgcagc tgcggggcca gcggtatgtg ccgtcatcgc gtgatgctgg tgttaagtta
    17101 tcaacgactt tgtgctaccg cccagcctac gggaaaagaa gaggaatggg atccggcaat
    17161 ctggctggaa gaactggcta cccttcccga tgccacccgc aaacgcgcac aggcgctggt
    17221 cgctaaaggc atcaccattg agttgttctg tgcgccgggt gaaattccct ctgcccgctt
    17281 accgatgagc gatgtgcgtt tttattcccg cagcagtatt cgtttcgccc gttgtgattg
    17341 tattgaaggc acactttgcg aacat
//
LOCUS       DAJVUJ010000087        17347 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.087, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000087 DAJVUJ010000000
VERSION     DAJVUJ010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17347
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.087"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            193..2040
                     /gene="ldtD"
                     /locus_tag="OQQ48_002546"
     CDS             193..2040
                     /gene="ldtD"
                     /locus_tag="OQQ48_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCS1953276.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGAGGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2221..2769
                     /locus_tag="OQQ48_002547"
     CDS             2221..2769
                     /locus_tag="OQQ48_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HCS1953277.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2796..3443
                     /gene="gloC"
                     /locus_tag="OQQ48_002548"
     CDS             2796..3443
                     /gene="gloC"
                     /locus_tag="OQQ48_002548"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HCS1953278.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3665..4855)
                     /gene="aspC"
                     /locus_tag="OQQ48_002549"
     CDS             complement(3665..4855)
                     /gene="aspC"
                     /locus_tag="OQQ48_002549"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HCS1953279.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARMAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(5040..6128)
                     /gene="ompF"
                     /locus_tag="OQQ48_002550"
     CDS             complement(5040..6128)
                     /gene="ompF"
                     /locus_tag="OQQ48_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HCS1953280.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6730..8130)
                     /gene="asnS"
                     /locus_tag="OQQ48_002551"
     CDS             complement(6730..8130)
                     /gene="asnS"
                     /locus_tag="OQQ48_002551"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCS1953281.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(8299..9501)
                     /gene="pncB"
                     /locus_tag="OQQ48_002552"
     CDS             complement(8299..9501)
                     /gene="pncB"
                     /locus_tag="OQQ48_002552"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCS1953282.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYKKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9767..12379
                     /gene="pepN"
                     /locus_tag="OQQ48_002553"
     CDS             9767..12379
                     /gene="pepN"
                     /locus_tag="OQQ48_002553"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HCS1953283.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPEHFTLKIVNEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVISLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQFHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            complement(12422..13173)
                     /gene="ssuB"
                     /locus_tag="OQQ48_002554"
                     /pseudo
     CDS             complement(12422..13173)
                     /gene="ssuB"
                     /locus_tag="OQQ48_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309043.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
     gene            complement(13170..13907)
                     /gene="ssuC"
                     /locus_tag="OQQ48_002555"
                     /pseudo
     CDS             complement(13170..13907)
                     /gene="ssuC"
                     /locus_tag="OQQ48_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001714028.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
     gene            complement(13972..15117)
                     /gene="ssuD"
                     /locus_tag="OQQ48_002556"
                     /pseudo
     CDS             complement(13972..15117)
                     /gene="ssuD"
                     /locus_tag="OQQ48_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
     gene            complement(15114..16073)
                     /gene="ssuA"
                     /locus_tag="OQQ48_002557"
     CDS             complement(15114..16073)
                     /gene="ssuA"
                     /locus_tag="OQQ48_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HCS1953284.1"
                     /translation="MRNIIKLALVGLLSVSTIAVAAESSPEALCIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            complement(16066..16641)
                     /gene="ssuE"
                     /locus_tag="OQQ48_002558"
     CDS             complement(16066..16641)
                     /gene="ssuE"
                     /locus_tag="OQQ48_002558"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HCS1953285.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFVP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQVIDYHHRPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            16997..>17347
                     /locus_tag="OQQ48_002559"
     CDS             16997..>17347
                     /locus_tag="OQQ48_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1953286.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGKVTFNGKVIAPACT
                     LVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADG
                     VQPTAFANQATTDAA"
BASE COUNT         4373 a         4440 c         4399 g         4135 t
ORIGIN      
        1 cataacggcg cgcttttctt taaacttctt tacattaggt tatgtaaaaa cgtatcggcg
       61 tttatatact gaagataagc ctgatgagta acaggcttgc tcgtcatact ttcgtgagta
      121 ttggcgttgt acaggcaagt cgtaaaataa cagcctggct attcagagta tgataaaaac
      181 agggggcaag ggatgttgct taatatgatg tgtggtcgtc ggctgtcggc aatcagtttg
      241 tgcctggccg taacattcgc tccactgttc aatgcgcagg ccgatgagcc tgaagtaatc
      301 cctggcgaca gcccggtggc tgtcagtgaa cagggcgagg cactgccgca ggcgcaagcc
      361 acggcaataa tggcggggat ccagccattg cctgaaggtg cggcagaaaa agcccgcacg
      421 caaatcgaat ctcaattacc cgcaggttac aagccggttt atcttaacca gcttcaactg
      481 ttgtatgccg cacgcgatat gcaacccatg tgggaaaacc gtgatgctgt taaagccttc
      541 cagcaacagc tggcagaggt ggcgattgcc ggtttccagc cgcagtttaa taaatgggta
      601 gagttactga ccgatcctgg tgttaacggg atggcacgcg acgtagtgct ctctgatgcg
      661 atgatgggct atctccattt cattgcaaat attccggtca aaggcactcg ctggctatat
      721 agcagtaaac cttatgcact tgcaacgccg ccgctctcgg tgattaacca atggcagctg
      781 gcgctggata aaggtcaatt gcctacgttt gttgcaggac tggcaccgca gcatccgcaa
      841 tatgcggcga tgcatgaatc gttactggcc ttactcagtg acaccaaacc gtggccccaa
      901 ctgaccggca aagcaacgtt gcgcccaggg cagtggagta acgacgtacc ggcgttgcgc
      961 gaaatattgc aacgcacagg catgttggac ggggggccga aaattactct acctggcgat
     1021 gacacgccaa ctgacgcggt agtcagccca tccgctgtta ctgttgaaac agcagaaact
     1081 aagccgatgg acaagcaaac gacgtctcgt agtaaacctg cgcctgccgt tcgcgccgcc
     1141 tacgataatg aactggtgga agccgttaaa cgttttcagg catggcaagg attgggggca
     1201 gatggtgcta ttggcccggc aacacgtgac tggttaaacg taacgcccgc ccagcgtgct
     1261 ggcgtgttgg ctctcaacat ccagcgattg cgcttgctgc caacagagct ttctaccggg
     1321 atcatggtta acattccggc ctattcgctg gtctactatc agaacggcaa tcaggtgctg
     1381 gattcgcgag tcattgtcgg tcgccccgat cgcaaaacgc cgatgatgag cagtgccctt
     1441 aacaacgtag tggtaaaccc gccgtggaac gtacctccaa ctctggcacg caaagatatt
     1501 ctgccaaaag tgcgcaacga tccgggatat ctcgaaagcc atggctatac ggtgatgcgt
     1561 ggctggaaca gcagagaagc gattgaccca tggcaggttg actggtctac aatcacggcc
     1621 tcgaatttac cgttccgctt ccagcaggct ccaggcccac ggaactcgct ggggcgctat
     1681 aaattcaata tgccgagttc agaggccatt tatttgcatg acacgccgaa ccacaatctg
     1741 ttcaagcgtg atacacgcgc attgagctca ggctgtgtac gagtgaataa agcttccgat
     1801 ctggcgaata tgctgttgca ggatgcaggc tggaatgaca aacgtatttc tgatgcgctg
     1861 aagcagggtg atacacgtta cgtcaatatt cggcagtcga ttccggtgaa tctctactac
     1921 ctgacggcct ttgttggtgc aggtggtcgt acccagtatc gtacagatat ttacaattat
     1981 gatctgcctg cgcgatccag ctcgcaaatc gtatcgaaag cggaacaatt aatcaggtaa
     2041 atgaagtagt tcggggaaat cagttgtcgt aataattagc atgattgggg gcgattctct
     2101 gcagcccccg tcactgctgg ggttgagtca tcttgacgtc tgctttacgg gcggttaagg
     2161 tgcctcttgt gcgccagaag tgcatataaa cgataacatt gacctgtaga cttgattatc
     2221 atggacaaat tcgacgctaa tcgccgcaaa ttgctggcgc ttggtggcgt tgcactcggt
     2281 gccgccatcc tgccgacccc tgcgtttgca acactctcta ccccacgccc gcgcattttg
     2341 acactcaata atcttcatac cggagagtca atcaaagcgg agtttttcga tggcagaggc
     2401 tatattcagg aagaattggc aaaacttaac cattttttcc gcgattaccg cgcgaacaaa
     2461 ataaagtcca tcgacccagg attattcgac cagttgtatc gcctgcaagg gttgttaggc
     2521 acgcgcaaac cggtgcaact catttccggt tatcgttcca ttgataccaa caatgaacta
     2581 cgcgcccgca gccgtggagt agcgaagaaa agctatcaca ctaaaggcca ggcgatggat
     2641 ttccatattg aaggtatcgc gttaagcaat attcgcaaag ccgcgttatc tatgcgcgca
     2701 ggtggtgtag gatattaccc acgtagtaac tttgtgcata ttgataccgg gccagcacgg
     2761 cactggtagc aatcgcttaa cgaaacaggg gcagtatgaa ctatcgtatt attccggtca
     2821 ccgcattctc ccagaactgt tcattaatct ggtgtgaaca aacccgtctg gccgcactgg
     2881 tcgatcctgg cggcgatgcg gaaaaaatca aacaggaagt tgatgacagc ggcctgacac
     2941 tgatgcagat cctgctgacg catggtcatc tggaccacgt tggcgcagcg gcggaactgg
     3001 cgcaacatta cggcgtgccg gtttttggcc cggaaaaaga agatgagttc tggctgcaag
     3061 gcttgcctgc gcaaagtcgt atgtttggtc tggaagagtg ccagccgctg acgccagatc
     3121 gttggctgaa cgaaggcgat accatcagca taggaaatgt gactttacag gtgttacatt
     3181 gccctgggca tacgccgggt catgtcgtgt tttttgatga tcgggcaaag ctgctgattt
     3241 ctggcgatgt tattttcaaa ggcggagtag ggcgcagtga cttcccgcgt ggcgatcata
     3301 atcaactgat ttcttcaatc aaagataaat tgctgccact gggggatgac gtgacattta
     3361 ttccgggtca cggaccatta tccacacttg gttatgaacg cctgcataat cccttcctgc
     3421 aagacgaaat gcccgtctgg taaggcacat aaaaaagccc gcttttaatg cgggcctgga
     3481 tttctggcaa agtgcgcttt gtttatgccg gatgcggcac gagcgcctta tccggcctac
     3541 aaaatcgtgc aaattcaaaa tattgcaggg gacgcgtagg cctgataagc gtagcgcatc
     3601 aggcaatgtt gcgtttgtca tcagtctcag cccgcttttc agcgggcttc attgttttta
     3661 atgcttacag cactgccaca atcgcttcgc acagcggagc catgttatct ggtgtcatcc
     3721 cggccacatt tacgcgacca gaagcaaccg catatacgcc aaactcttcg cgcagacgca
     3781 gcacttgttc ttttgtcagg ccactgaagg agaacatgcc gttctgtttg atgataaagc
     3841 tgaagtcgcg gtttgcgcct ttttcctgca gcgtattgac gaacaactga cgcatacgct
     3901 gaatacgctg gcgcatatca gtcagctctt gttcccaaat cgcacgtaac gcatcgttgc
     3961 tcaggatggt ggcaacaaca gaagcgccgt gtgctggtgg gttagagtag ttagcgcgaa
     4021 tcgccgcttt catttggctg aatgcgcgat caacggtttc actgtcggca gcaaccagag
     4081 tacaagcgcc aacacgctcg ttgtacaggc caaagttttt agagtaggaa ctggcaacaa
     4141 tcagctcttt atgcatagcc gcgaaagcgc gcagtccttc agcatcttct tccagaccac
     4201 gggcaaaacc ctggtaagcg aagtcaaaca gcggtaacca gcctttctca acggagagtt
     4261 gtgccagtgt ctgccattgt tccagcgtag ggtcgatacc ggttgggtta tggcagcagc
     4321 catggaacag cactacgtcg ccagcctgag cttcattcag gctgttaatc agtgcatcga
     4381 agtcaagagt gtgattttcc gcatcataat aagcgtattc acgaacttcc agacctgcag
     4441 agttaaagac gctcttatgg ttcggccagc ttgggttgct cacccacact cgcttaacgc
     4501 tggtattttt tgccaggaaa tcggcagcta cgcgtagtgc gccagtgccc cccggagtct
     4561 gtgccatgcg agcacgtttg tcattgatca gggcgctacc tttaccaaac agcagttcct
     4621 gagtgcagcg accaaattca gggatgccgt caatgccgag gtaatttttg gtggtttcat
     4681 tttcgagcag atactgttca gcctttttca cgctggtcag taccggggtt ttgcccgtct
     4741 catctttata gacaccaatc ccgaggttaa ttttgccggg acgttcatcg gcacgaaaca
     4801 gatcggccag gcccagaatc gggtcggcag gagcggcggt aatgttctca aacatgacga
     4861 ggttccatta tggttacaga agggaagtcc gctatcaggg taacgggaga tttacaaaat
     4921 tccaactatt actgatgaaa acgcaggctg tttttgcaag acgtgagatt gctctggaag
     4981 gtataaaaaa aacaggacca aagtcctgtt ttttcggcat ttaacaaaga ggtgtgctat
     5041 tagaactggt aaacgatacc cacagcaacg gtgtcgtctg aacctacgcc cagtttgtta
     5101 tcagaatcga tctggttgat gatgtagtca acataggtgg acatgttttt gttgaagtag
     5161 taggttgcgc ccacttcaaa gtagttcacc agatcaacat caccgatacc ttctacgtct
     5221 ttcgctttag atttggtgta agcgatggac ggacgcagac cgaaatcgaa ctggtattgc
     5281 gcaactaaca gaacgtcttg cgttttgttg gcgaagccgc tggtgtttgt aaatttatta
     5341 gtgatcggcg tagcgttacg ggtttcaccg tagttcgctg ccaggtagat gttgttcgcg
     5401 tcgtacttca gaccagtagc ccactgttca gcttttttac cgttgccaag aggttgagct
     5461 tcttgcaggt tggtacggtc agctgcacca taagcaccaa cgataccaaa gccttcgtat
     5521 tcgtagctga tagaaccgcc aacaccgtcg ccgttagaac ggcgtgcagt gtcacgctcg
     5581 tttttaccca ggtactgaac agcgaagttc aggccatcaa ccagaccaaa gaagttggag
     5641 ttacgatagg tagcaacgcc gccaacacga ccaacgaaga agtcatcgct gtatgcagta
     5701 tcaccaccaa attctggcag catatcggtg taacccagtg catcataaac cacaccgtag
     5761 ttacggccgt aatcgaaaga accaacgtca gcgtatttaa gacccgcgaa tgccagacgc
     5821 gttttgttac cagtttgagc gtcagcgcct tcagagttgt taccttggaa gttatattcc
     5881 cactgaccat aaccggtcag atcggaattg atttgagttt cccctttaaa accaagacgg
     5941 gcataggtca tgtcgccatt gccaccgtaa ctgttttcac cgttaccctt ggaaaaataa
     6001 tgcagaccaa cagctttacc gtacagatct actttgttgc catctttgtt atagatttct
     6061 gcagcgtttg cagtacctgc tactaacaga gcagggacga tcactgccag aatattgcgc
     6121 ttcatcatta tttattaccc tcatggtttt ttttatgaca cctgccactg ccgtcaataa
     6181 gttctgtcaa taaaaattta cggaactatt gatgagagtt tggtgtcttt atgtgtctgc
     6241 aggcatcttt ccattcaaac taacgtttcg ctaccgtgaa agtgctgcaa agataaagat
     6301 ttagtttcaa aaagaaaaaa tatgtaacca aaagtaaaaa ttaaggaact ttgtgaacac
     6361 cgtcatattt ccatagaaac gtgatgatat ttacagcaat ttttatctat ttatatgatt
     6421 tacttatatt taaattaact aagcggaaat tttgtttctg atggaaactt tatcgacctg
     6481 gcacaaaatc ctctttcaga catccagaat gccaaaaaat agtatgaaaa ttgtgctatc
     6541 agctaagaaa aaactaatcc gcaataagaa tacgggttat tttttgtgcg taaatgttgc
     6601 aatgtaatat tccgtaacag gatgatcgtt atggcggggt tgcaaataga ttgcttgact
     6661 taacaaacag ataacttgac agaaaggata aaaaagagcc agcaggcgct ggctctttga
     6721 ggaagaaaat cagaagctgg cgttgcgcgg agtacgtggg aacggaatca catcacgcac
     6781 gttttgcacg ccagttacgt aagcaatcag gcgttcaaaa ccaagaccga aacctgaatg
     6841 cggaacagta ccgtagcgac gcagatcgcg ataccaccag taatcttctt tattcaggcc
     6901 catttccagc atacgctcgt ccagaacgtc cagacgttct tcacgctggg agcctccaat
     6961 gatctcaccg atgcccggag ccagaacgtc catagccgca acggttttac cgtcttcgtt
     7021 gaggcgcata tagaacgctt taatatcttt cggatagttt ttaaccacta ccggtgcttt
     7081 aaagtgttct tccgccagat aacgctcatg ctcagaagag agatcgacgc cccagtaaac
     7141 cgggttttca aacttcctgc cgcagttttc gagaatggtc actgcgtcgg tgtaatccac
     7201 ctgcgcgaaa tcggcctcaa tgaagcgttc cagacgtgaa acggcatctt tatctacgcg
     7261 ttcagcgaag aatttcatgt cgtctgcgcg ttcttcgaga accgctttga agacatattt
     7321 cagcatggct tcagccagac ccgcgatatc gttcaggtta gcaaacgcca cttccggctc
     7381 cagcatccag aattccgcca ggtgacggct ggtgttggag ttttcagcac ggaaagtcgg
     7441 gccgaaggta taaattttgg ataatgcgca agcgtaggtt tcgccgttca actggccaga
     7501 tacggtcagg aaagactctt taccaaagaa gtctttgtcg aaatccactt tgccctgatc
     7561 gttacgcggc aggttttcca gatccagcgt agaaacgcgg aacatttcgc ctgcaccttc
     7621 ggtatcagat gcggtaatca gtggcgttga aacccagaag aatccctgct cgttaaagaa
     7681 gcgatgcagc gcctgcgcca gcgtatggcg aacgcgcgcg acggcaccaa tcaggtttgt
     7741 gcgcggacgc aggtgagcga cttcacgcag atactcaatg ctgtggcgtt ttgccgccat
     7801 cgggtaagtg tctggatctt caacccaacc agcaacttca actttgctgg cctgaatttc
     7861 aaattgttgc ccctggcccg gcgacgccac gactttaccc gtcacaatga ccgagcagcc
     7921 ggtggtcaga cgcaggacgt cttcattgta attgggcaga gaattattga tgacagcctg
     7981 tacaggatca aagcaggaac cgtcataaac ggcgaggaag gagatgccag cttttgaatc
     8041 tcggcgggta cgtacccatc cgcgtacggt gacttcgctg tcaacggcta cgcggccctg
     8101 gagtacgtcg gctacaggca caacgctcat aatattctct ctgttaatag tcggaaaaaa
     8161 taaacacttg tccacccgta aatgggggga tacctatgtt acctggcatc tgcaatcaga
     8221 caagcagaat tcgcaaatgc agcgaaagat ttcggaatta aaggtaaaaa aagggagccg
     8281 attagctccc tgatgatatt aactggcttt tttaatatgc ggaaggtcga acgctttgcg
     8341 cagcgcccga acaaacgctt tatcatggca gatagttttg ccagggctgt cagaaagttt
     8401 cgccaccggt ttaccgttac actctaccaa cttaatcaca atattcaggg gttttacctg
     8461 ggggatatcg caggtcaggc gagtcccaat accaaaactt aattgcacgc gggaagagaa
     8521 gtggcggtat agctcaaccg ctttgcgtaa atccagattg tcagagaaaa ccagcgtttt
     8581 actctgtgga tcaattccca gctttttata atgtgcaatg gctttttcac cccattcaac
     8641 cgggtcgcca gagtcatgac gcaggccctg ataccgactg gcgaactcga caccgaaatc
     8701 acgcaggaaa gcatccatag tgatgcagtc ggttaatgca atgccaagtt ggtcgggata
     8761 ttcttccagc caggcagcaa gtgcagctcg ctggctgttg gctaggtccg ggctgatttg
     8821 ctgatgtgcc tggaaccatt cgtgcgcctg tgttcccatc ggcgtgaggg aaagccgacg
     8881 cgccagatcg tagttgctgg tgcccacgaa ccaggattcc tgttgcagac gcttaacgat
     8941 ggtttcttgt acttcgcgag aaaaacggcg acgggtgcca aaatccatca gatggaagcg
     9001 cgacatatca agaccggcgg ttaacgccga gaagtcgact aatttgcttt ccagcgtgtc
     9061 gagggcttgc gcaacgtcgg cctgcggtga gcgatagcga tgtaccattt cactgataac
     9121 cgccagcaaa ggaacttccc agaggatgac ttcacgccac ggaccgctta agcgaatatc
     9181 cagcttgcca ttatcgttgg acacggtgac ttgttccggg ttaaagcgga attcgcgtaa
     9241 ccagttaaga tagtcggcct taaagaaagg cagggcagag agccactgat attcatcatc
     9301 ctgcaggcgc aggtgctgca tcgcctgaat ctgttcacga atagcatcgg cataaatacc
     9361 cagcagatcg tcacctcggc aacgaaactc cgccgcgaca tgcacatcgt aatagtgatg
     9421 aaacacggct tgctgcatat gcaacttata agcatctgta tccagcaacg agtgcagaac
     9481 aggagaagcg aattgtgtca taggtgcgca gtagcatcct cgtacaggag cgtttagtac
     9541 aataaacatc ttcaggaact gctggagtat accttgtttc gcaatttatt gaaccccgat
     9601 cacaccatat gccacctttc tggtcgatgg cattacgcgg tgtatgttat aaaaatgtag
     9661 caataaaggc gtttgtacct gaaaagatga agattctgca tagcgcgatt tacgcaacag
     9721 gaatagactg aacaccagac tctataaaag atgctaaagg ttatttatga ctcaacagcc
     9781 acaagccaaa taccgtcacg attatcgtgc gccggattac cagattactg atattgactt
     9841 gacctttgac ctcgacgcgc aaaagacggt cgttaccgcg gtcagccagg ctgtccgtca
     9901 tggtgcatca gatgctccgc ttcgtctcaa cggcgaagac ctcaaactgg tttctgttca
     9961 tattaatgat gagccgtgga ccgcctggaa agaagaagag ggcgcactgg tcatcagtaa
    10021 tttgccggag cattttacgc ttaagatcgt taatgaaata agcccggcgg cgaataccgc
    10081 gctggaaggg ctttatcagt caggcgatgc gctttgcacc cagtgtgaag ccgaaggttt
    10141 ccgccatatt acgtattatc tcgaccgccc ggacgtgctg gcgcgtttta ccaccaaaat
    10201 tattgccgat aaaaccaaat atcccttcct gctttccaac ggtaaccgcg ttgcgcaagg
    10261 tgaactggaa aacggacgcc attgggtaca gtggcaggac ccgttcccga aaccgtgcta
    10321 cctgtttgcg ctggtggcag gcgactttga tgtactgcgc gacaccttta ccacgcgttc
    10381 tggtcgcgaa gtggcgctgg agctgtacgt cgatcgcggc aaccttgatc gcgcgccgtg
    10441 ggcgatgacc tcgctgaaaa actcaatgaa atgggatgaa gaacgcttcg gcctggagta
    10501 tgacctcgac atctatatga tcgtcgcggt ggatttcttc aatatgggcg caatggagaa
    10561 taagggtttg aatatcttta actccaaata tgtgctggcc cgcaccgaca ccgccactga
    10621 caaagattac ctcgatattg aacgcgttat cggccatgaa tatttccata actggaccgg
    10681 taaccgagtc acctgccgcg actggttcca gctcagcctg aaagaaggtt taaccgtctt
    10741 ccgcgatcag gaattcagct ctgaccttgg ttcccgtgca gttaaccgca tcaataatgt
    10801 gcgcaccatg cgcggattgc agtttgcgga agacgccagc ccgatggcgc acccgatccg
    10861 cccggacatg gtcattgaga tgaacaactt ctataccctg accgtttacg agaagggcgc
    10921 ggaagtgatc cgtatgatcc acaccctgct gggcgaagaa aacttccaga aagggatgca
    10981 actctatttc gagcgccatg atggcagcgc ggcaacctgc gacgactttg tgcaggcgat
    11041 ggaagatgcg tcgaatgtcg atctctccca tttccgccgt tggtacagcc agtccggcac
    11101 gccgattgtg accgtcaaag acgactacaa tccggaaacc gagcagtaca ccctgaccat
    11161 cagccagcgc acgccagcca cgccggatca ggcagaaaaa cagccgctgc atattccgtt
    11221 tgccatcgaa ttgtatgaca acgaaggcaa agtgatctcg ttgcagaaag gcggtcatcc
    11281 ggtgaattcc gtgctgaacg tcacccaggc ggaacagacc tttgtttttg ataatgtcta
    11341 cttccagccg gtgcctgcgc tgctgtgcga attctctgcg ccagtgaaac tggaatataa
    11401 gtggagcgat cagcaactga ccttcctgat gcgtcatgcg cgtaatgatt tctcccgctg
    11461 ggatgcggca caaagcctgc tggcaaccta catcaagctg aacgtcgccc gacatcagca
    11521 agggcagccg ctgtctctgc cggtacatgt ggctgacgct ttccgcgcag tgctgctcga
    11581 tgagaagatt gatccggcgc tggcggcaga aatcctgacg ctgccttctg tcaatgaaat
    11641 ggctgaactg ttcgatatca tcgacccgat tgctattgcc gaagtacgcg aagcactcac
    11701 tcgtactctg gcgactgaac tggcggatga gctgctggct atttacaacg cgaattacca
    11761 gagcgagtac cgtgttgagc atgaagatat tgcgaaacgc actctgcgta atgcctgcct
    11821 gcgcttcctt gcttttggtg aaacgcatct ggctgacgtg ctggtgagca agcagttcca
    11881 cgaagcgaac aatatgaccg atgcgctggc ggcgctttct gcggcggttg ccgcacagct
    11941 gccatgccgt gatgcgctga tgcaggaata cgacgataag tggcatcagg acggtctggt
    12001 gatggataaa tggtttatcc tgcaagccac cagcccggcg gcgaatgtgc tggagacggt
    12061 gcgcggtctg ttgcagcatc gctcatttac catgagcaac ccgaaccgta ttcgttcgtt
    12121 gattggcgcg tttgcgggca gcaatccggc agcgttccat gccgaagatg ggagcggtta
    12181 ccagttcctg gtggaaatgc ttaccgacct caacagccgt aacccgcagg tggcttcacg
    12241 tctgattgaa ccgctgattc gcctgaaacg ttatgatgcc aaacgtcagg agaaaatgcg
    12301 cgcggcgctg gaacagttga aagggctgga aaatctctct ggcgatctgt acgagaagat
    12361 caccaaagca ctggcttgat aaataaccga atggcggcaa tagcgccgcc attcggggaa
    12421 tttacccctg ttttctcagg cgaatttcag attcaccacg ctgcatcacc ctttgcaaca
    12481 cttccgattc cagctccgct aatcgcaccg accctaatcg acgcggaggg gggatatcca
    12541 ctgtcaaatc cagaccaatt tttccctctt caattaataa cacccggtca gccattgcta
    12601 ccgcttcgct cacatcatgc gtcaccaaca gtacggtaaa gccgtgctcc tgccaaagtg
    12661 acacaatcaa atcctgcatc tcgagtcgcg ttaaggcgtc cagtgccccg agcggttcat
    12721 caagcaacaa taaacccggt cgatgaatca acgctcttgc cagcaccact cgctgtttct
    12781 gcccgcctga aagtgcggca ggccattccc cggcgcgatt ctccagtcct accgcagcca
    12841 acgcccgacg cgcggcatcg cgccactggc ctttaaggcc taacccaacg ttatcaatca
    12901 ccgatttcca cggtagcaga cgcgcatcct gaaacatcat tcgcgtgtct tcctgaattt
    12961 cagccagcgg tgtggtgcct gccaacacat cgcctgcggt tggcgtttcc agcccggcca
    13021 gcaggcgcag cagggtactt ttgccaccac cgccacaaac tgacctgccg gaatatgtaa
    13081 atccagttgg ttcaggacga tattttccgc gtaatgtttg cttactgcat tgagcaacaa
    13141 tggtgtgccc tggttcagac gagcagtatt cataccgtgg cctccttcaa atgataagcc
    13201 gggttccagc gcaaccagag gcgctctaac agctgcgcgc tgacgtcagc aagtttgccg
    13261 agcagggcgt aaagaataat agcgactacc accacgtccg tttgcaaaaa ctctcgcgca
    13321 ttcatcgcca gataaccaat gccggaattg gcagaaatgg tttcggcaac aatcagcgtc
    13381 agccacatca ggcccaacgc aaaacgcacg ccgaccataa ttgagggcag ggcaccaggc
    13441 aggatcacat ggataaacag cggtatgccg gataacccat agctacgcgc catctccacc
    13501 agcccgcgat cgatattacg gatgccatgc caggtgttga tataaatggg aaacagcgta
    13561 ccgagcgcca ccagaaagat ttttgcggat tcatcaatgc caaaccacaa aatcaccagc
    13621 gggatcagcg ccagatgcgg cacgttgcgc aacatctgaa ttgaggtatc cagcagccgt
    13681 tctccccagc gagatagccc gctaataagt cccaaaatca accccagcga tccgccaatt
    13741 gaaaagccaa tcagcgcccg ctaggaactg atcgccagat gctgccacag ttcgccgctg
    13801 gcggagagcg tccagaacgc cgtcaccacc ccttccggtg agggcaaaat acgcgtcgac
    13861 agccagccaa ctgaggaggc cagttgccac accgccacga tgcccaccgg taaaaactag
    13921 ggggcaacgc gcaataacca cttcttcact ggcgttgcca ttatttcttc cttagctttg
    13981 cgcgacttta cgggggataa aatcattcgc caccgcttcg ccttgcggat tcagcggctg
    14041 cggctgggga atttccggaa tggcgacatc cagatgtggg aacagcaact cgccgacccg
    14101 atacgcttct tccagatgcg gatagcccga aagcacaaag ctgtcgatgc caagcgcggc
    14161 gtattcgttg attcgcgcag cgaccgtagg accatcgccc accagcgccg ttccggcacc
    14221 gccgcgcact aagccaacgc ccgcccataa attggggctg atctccagat tgtcgcgctt
    14281 gccgttatgt aacgccgcca ttcgctgttg tcctacggaa tccgtccggg cgaatgcggc
    14341 ctgtgctttg gcgatagttt catcatcaag atgcgagatt aaccgctcgg cggcctgcca
    14401 cgcttcgtcg ttagtttcac gaacaatcac atgcagacga ataccgaaac gaattttgcg
    14461 tccatgcgcg gcagctttcg cccgcacttg ttcgatcttc tcttttacta gttctggcgg
    14521 ttcgccccag gtgaggtaga gatcaacttg ttctgccgcc agctcctggg cgacgtctga
    14581 cgatccgcca aagtaaagcg gaggataagg ctgttgaatc ggcgggaaga gcagttttgc
    14641 tccgcgcaca tgaatatgtt taccgttgaa attgacggtt tcccccagca ataagcgccg
    14701 ccagacctag gtaaattccg ccgaggcttc gtagcgctcg ctatgatcaa ggaacacgcc
    14761 gtcgcctgcc agctcttgtg gatcgctgcc tgtgaccaga ttaaacaacg cacgaccatt
    14821 tgagagacgg tcaagcgtgg cggcctggcg agcggcaacg gtaggtgagg ttacgctggg
    14881 gcgcagggcg acaagaaact tcagccgctg cgtcaccggg atcatcgacg ccgccaccag
    14941 ccacgcatct tcacacgatc gccccgttgg gatcagcaca ccggtatagc ccagacgatc
    15001 cgccgcttgc gcaatttgtt gcagataacc gtgatcaacc gggcgtgaac cttcttccgt
    15061 tcccagatag tgcccgtcac cgtgggtcgg taaaaaccag aacatattca gactcataat
    15121 tgttttcctt ccagttgagt gggctgccag atgcgctggc gaatatcgac ttttttcggc
    15181 accagacgat tttcataaaa cagatctgcc gtttgctgct gtaaggcggc aacctcggcg
    15241 ttaaccggtt tgatggtggt aggagggcga tgatctaagt aagaggcaat caccggtgcc
    15301 ggtaagccca tcgtttttgc cagtaaagcg atgctttgct cgcgctggct gcgggttaac
    15361 gcatcggcct cactaaaggt tgccagtacg ccctgaataa aagcgccgtt tttttctgca
    15421 tagggacgag ctgccagata aaacgatcca gtttgattga gatcggtgcc gtctttcagc
    15481 acccgcacgc cgccctgtaa taatgcagca gagtagtagg gatcccagat agcccaggcg
    15541 tcaacgttac cttgctggaa cgcggcgcgg gcatcagcgg gcgtcaggta agtgggttgg
    15601 atatcggtaa acttaagtcc ggcctgacgc agtgcacgca gtaaaaggtt gtgtgaactg
    15661 gaacctttct gaaaggcaac tttgtgacct ttaagatcgg ctacggtttt gatcgggctg
    15721 ttttctgcca ccagaatcac ttcggctttg ggcttcggtg gctcgacgcc cacatatacc
    15781 aaatcagccc cggcagcctg ggcaaagatt ggcggaatat ccccggtact gccgagatca
    15841 atactgccaa cgtttaaggc ttccaacatt tgcggacccg cggggaactc cacccaggag
    15901 atttttgatt gcggatagcg tttttccagt aactggtggc tttttgccag taccatacca
    15961 atactgcctt tctgatagcc tatacataac gcttcaggcg aggattctgc agcaaccgca
    16021 atcgtagaga cgctaagcaa tcccaccagc gccagtttaa tgatgttacg catgggcatt
    16081 acctcgcaga gacagaaggt caggaacctg aacatcgcgg cggtgcaatg cctgccagaa
    16141 agtttctagc gcggtatcaa gacgggtttg cagatttggc gtgaactggg gtctgtgatg
    16201 gtaatcaatt acttgtgagt catcggcaaa cacgccgtgc aggatctcct gagctttcag
    16261 tgcgcttaaa actggtttaa gggcataatc gaccgccagc agatgggcca cggtaccgcc
    16321 cgtcgccagc ggtagcacca ctttgccttg caaagcgcgc tctggcagca ggtcgagcag
    16381 ggttttcagc gcgccggaat aggcggcttt atacacaggt gtagcgacta tcagtccatc
    16441 agcctgttgc agctgttcgg tgaaggtctt gagtgccgga ctatcgaaac gagcataaag
    16501 cagatcttcc gggacgaagt tttgcagatt ccagtgataa acctctacat ccaggccatt
    16561 tagtttttcc cgcgcatatt ccagcaagga gctggagcga gaaggaaagc gaggactacc
    16621 cgccagggtg atgacacgca tactctctcc ttataaccaa ttgttctttt tttgttaaca
    16681 ttgataacaa ttcggtcagt ctgtcggaga gacaagaaaa tgccaaatat aaattttgtg
    16741 tatctttttc tgaaaataaa ctaaagaaag ggctttatat atcacgcata tttatttgtt
    16801 ggatagtcat tagatgttga atcaatgaat tactcgttca gttaatattt aaatattcat
    16861 gaaatctata aattaaagat ttgtcactta ttggatttag tatttttgat ttaaatgacg
    16921 gtcaattatt gttattatta agtctaatgt catttaaagg cattatcata tttacgaaga
    16981 gatcaggatg atgacgatga aaaaaagtgt attgacggcg tttataactg tggtatgtgc
    17041 aacgtccagc gttatggcag ctgatgataa tgctatcacg gatggcaaag taacatttaa
    17101 tggtaaagtt attgctccag cttgtaccct ggtagctgcg acgaaagatt cagtagtcac
    17161 attgccaaat gtcagcgcca ccaagttgca aactaatgga gcagtctctg gcgttaaaac
    17221 tgatgtacca attgccttag aaggctgtga tgttactgta actaaaaatg cgacgtttac
    17281 ctttagtggt acggctgacg gcgtgcaacc tacagcattt gccaaccagg ctaccaccga
    17341 tgcagcg
//
LOCUS       DAJVUJ010000088        17015 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.088, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000088 DAJVUJ010000000
VERSION     DAJVUJ010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17015
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.088"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(<1..304)
                     /gene="hlyE"
                     /locus_tag="OQQ48_002560"
     CDS             complement(<1..304)
                     /gene="hlyE"
                     /locus_tag="OQQ48_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HCS1953287.1"
                     /translation="MTEIVADKMVEVVKNAIETADGALDLYNKYLDQVIPWQTFDETI
                     KELSRFKQEYSQAASVLVGDIKTLLMDSQDKYFEATQTVYEWCGVATQLLAAYILLF"
     gene            677..1096
                     /locus_tag="OQQ48_002561"
     CDS             677..1096
                     /locus_tag="OQQ48_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HCS1953288.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            1096..2363
                     /gene="umuC"
                     /locus_tag="OQQ48_002562"
                     /pseudo
     CDS             1096..2363
                     /gene="umuC"
                     /locus_tag="OQQ48_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
     gene            complement(2409..2939)
                     /gene="dsbB"
                     /locus_tag="OQQ48_002563"
     CDS             complement(2409..2939)
                     /gene="dsbB"
                     /locus_tag="OQQ48_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HCS1953289.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     MISQPFKAKKRDLFGR"
     gene            complement(3085..4626)
                     /gene="nhaB"
                     /locus_tag="OQQ48_002564"
     CDS             complement(3085..4626)
                     /gene="nhaB"
                     /locus_tag="OQQ48_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HCS1953290.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            4848..5567
                     /gene="fadR"
                     /locus_tag="OQQ48_002565"
     CDS             4848..5567
                     /gene="fadR"
                     /locus_tag="OQQ48_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HCS1953291.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(5619..7151)
                     /locus_tag="OQQ48_002566"
     CDS             complement(5619..7151)
                     /locus_tag="OQQ48_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HCS1953292.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSVELLERCPPRMG
                     NL"
     gene            7481..8779
                     /gene="dadA"
                     /locus_tag="OQQ48_002567"
     CDS             7481..8779
                     /gene="dadA"
                     /locus_tag="OQQ48_002567"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HCS1953293.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFDSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            8789..9848
                     /gene="dadX"
                     /locus_tag="OQQ48_002568"
                     /pseudo
     CDS             8789..9848
                     /gene="dadX"
                     /locus_tag="OQQ48_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
     gene            complement(10235..11971)
                     /locus_tag="OQQ48_002569"
     CDS             complement(10235..11971)
                     /locus_tag="OQQ48_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HCS1953294.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGTAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(12066..12980)
                     /gene="ldcA"
                     /locus_tag="OQQ48_002570"
     CDS             complement(12066..12980)
                     /gene="ldcA"
                     /locus_tag="OQQ48_002570"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HCS1953295.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNKTFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVSAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            13080..13691
                     /gene="emtA"
                     /locus_tag="OQQ48_002571"
     CDS             13080..13691
                     /gene="emtA"
                     /locus_tag="OQQ48_002571"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HCS1953296.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(13693..14427)
                     /locus_tag="OQQ48_002572"
     CDS             complement(13693..14427)
                     /locus_tag="OQQ48_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HCS1953297.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            14628..14882
                     /locus_tag="OQQ48_002573"
     CDS             14628..14882
                     /locus_tag="OQQ48_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCS1953298.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFQTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGLFRLLRRE"
     gene            15061..15501
                     /locus_tag="OQQ48_002574"
     CDS             15061..15501
                     /locus_tag="OQQ48_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1953299.1"
                     /translation="MIQKINILDSSQSGVKNGADSEAVLAALIEHLELINPSGRLSQN
                     TRSAMLQLREEEWSEFFFWLLNSLECLDYVIINLTPESKKTLMSEHRNNIQVAIDALY
                     RQRRCKSPGDDSETLTRRNDAIFGNHVWQTFAQYFPPGLEKPAV"
     gene            complement(15580..17015)
                     /gene="treA"
                     /locus_tag="OQQ48_002575"
                     /pseudo
     CDS             complement(15580..17015)
                     /gene="treA"
                     /locus_tag="OQQ48_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="alpha,alpha-trehalase TreA"
BASE COUNT         4285 a         4203 c         4399 g         4128 t
ORIGIN      
        1 caaatagcaa aatatacgct gcgagcaatt gcgtcgcaac accacaccat tcatacactg
       61 tttgggttgc ttcaaaatac ttatcctggc tatccataag taaggtttta atatcgccga
      121 ctaaaacgga ggctgcctgt gaatactcct gtttaaagcg acttaactct tttatggttt
      181 catcaaaggt ctgccagggg atgacctgat cgagatattt attataaaga tctaatgctc
      241 catctgcggt ttcgattgcg tttttaacta cttctaccat tttatctgca acgatttcag
      301 tcataatcat tcgcctcttt aaatatataa attgtaatga aactcctgtt ttacaactat
      361 taataaattt tacttcatct aattcatagt tagccgggtg ggatgcgtca atgtctttat
      421 ttctattaat atgataaata tcaaacaatg tttaatgtca ttatggcgaa tgcttctatt
      481 ctattttttt gccgggtgat atttttcatt tctgctggat gagcgtcgtc gccagaaggc
      541 cacgtgagca caggataaga gaacgaaaaa tcagcagtct atacagcgac aaatattgat
      601 agcctgaatc agtattgatc tgctggcaag aatagactac tgtatataaa aacagtataa
      661 cttcaggcag attattatgt tgtttatcaa gcctgcggat ctccgcgaaa ttgtgacttt
      721 tccgctattt agcgatcttg ttcagtgtgg ctttccttca ccggcagcag attacgtcga
      781 acagcgcatc gatctgaatc aactgttgat ccagcatccc agcgcgactt acttcgtcaa
      841 agcaagtggt gattctatga ttgatggtgg aattagtgac ggtgatttac tgattgtcga
      901 tagcgctatt accgccagcc atggtgatat tgtcatcgct gctgttgacg gcgagtttac
      961 ggtgaaaaaa ttgcaactac gcccgacggt acagcttatt cccatgaaca gtgcgtactc
     1021 gcccattacc atcagtagcg aagatacgct ggatgtcttt ggtgtggtga tccacgtcgt
     1081 taaggcgatg cgctgatgtt tgctctctgt gatgtaaacg cgttttatgc cagctgtgag
     1141 acggtgtttc gccctgattt atggggtaaa ccggtggttg tactatcgaa taatgacggt
     1201 tgcgttatcg cccgaaacgc tgaggcaaag gcgctgggtg ttaaaatggg cgatccctgg
     1261 ttcaaacaaa aagatctgtt tcgtcgctgt ggtgtggttt gctttagcag caattatgag
     1321 ctttacgcag acatgagcaa tcgggttatg tcgacgctgg aagaactatc gccccgcgtc
     1381 gagatttaca gtattgatga ggcattctgc gatctgacag gtgtgcgtaa ttgtcgcgat
     1441 ctgactgatt ttggcagaga aattcgcgca acggtgctac aacgtaccca tcttactgtt
     1501 ggtgtgggga tcgcccagac caaaacgctg gctaagcttg ccaatcatgc ggcaaaaaaa
     1561 tggcagcggc agacgggggg gtggtggatt tatcaaatct ggaacgccag cgtaaattaa
     1621 tgtctgctct ccccgtggat gacgtctggg ggattggacg gcggatcagc aaaaaactgg
     1681 acgcgatggg gatcaaaacc gttcttgatt tggcggatac agatatccgg tttatccgta
     1741 aacattttaa tgtcgtgctc gaaagaacgg tgcgtgaact gcgcggtgaa ccctgtttgc
     1801 aactggaaga atttgcaccg acaaagcagg aaattatctg ttcccgctcg tttggtgaac
     1861 gtatcacgga ttatacgtcg atgcggcagg ccatttgtag ttacgctgcc cgggcggcgg
     1921 aaaaacttcg cagtgagcat caatattgtc ggtttatctc cacatttatt aagacgtcac
     1981 catttgcgct caatgaacct tattacggca atagcgcgtc ggtaaaactg ctgacgccca
     2041 ctcaggacag cagggatatc attaacgccg ctacgcgatc tctggatgcc atctggcaag
     2101 cgggccatcg ctaccaaaaa gcgggcgtga tgctggggga tttcttcagt cagggagtcg
     2161 cgcagctcaa tttattcgat gacaacgcac cgcgccccgg gagtgagcaa ttgatgacgg
     2221 taatggatac actgaatgct aaagagggca gaggaacact ctattttgcc gggcagggga
     2281 tccaacaaca atggcagatg aagcgagcca tgctttcacc acgttataca acgcgaagtt
     2341 ctgatttact gcgggtcaaa taaatatagc ggcaggaaaa aagtgctccc gcaggagcgc
     2401 cgaatggatt agcgaccgaa cagatcacgt tttttcgctt taaacggctg ggaaatcatc
     2461 accagcaccg cgacaatcag gtaagcgata aaaataccga gcagccactg cggcatttcc
     2521 agacctaaaa aatcccactg acgctcggcg caatcgccag aggcgacaaa cacttgcggc
     2581 acccatttat ccagcggcag ccattccggg aaacgaacca taaaatcaca ggtggcaaac
     2641 ggcgaaggat agagctgaag catggtgtgc tcgtaagtta actgcacacc gcggaacgca
     2701 ctatacaacc agataaccat cgctacataa cgcagcggag ttttcggggc gatcgcgcca
     2761 atcagcgcag cacccagaac gccgaataac gcgcagcgtt cataaataca gagcacgcaa
     2821 ggtttcagta acatcacatg ctggaaccac agcgccgtca gttccagtgc cagagcagta
     2881 aacgccatca acagccacgc gccccggcct tgtgaacatt ggttcaaaaa tcgcaacata
     2941 atcatttccc tgcaatatgc atagagtgcg cagtttaaac caattcattc gctgcgccac
     3001 caggaggcag caaaaatagg acgcaattgg acaattatcg ggcgaaaagg caaaccgggc
     3061 atttatatgc ccggtaagtt gttatcaaag cgttgctatc cagcccattt gcataaacca
     3121 ttcggttaca ggggcaagcg taaactcgac gcagagcagc ccgacgagtg tcaggacgag
     3181 ggtgtaaggc agggccatcc acaccatgcg gccataagag aggcgaatca atggcgcgag
     3241 tgcagaggtc agcaggaaca ggaacgcagc ctgaccgttc ggcgtagcga cagagggcag
     3301 attggtaccg gtattaatgg cgaccgccag cagctcgtat tgcttcaacg tgatagcgcc
     3361 actttccatt gccgctttcg cttcgttgat ataaatcgtc cccacgaaga cgttatccga
     3421 aatggatgac agcagaccgt tgaaaatata gaacaacgac agctgagcat gttccgatgc
     3481 ctgcaacaca aactgaataa tcggcgaaaa cagttgttgg tcgataatca ccgcgactac
     3541 cgagaaaaac accgtcaaca gtgcggtgaa tggcagagat tcggtgaagg ctttaccgat
     3601 agcatgttca tcggtgacac cggtcaatga tgtcgccaga ataatgaccg acaaaccaat
     3661 caagccaacc tccgccagat gcaacgccag cgcagtcacc agccagacgc cgataatcgc
     3721 ctggacaatc agacggattt tatcctgacg ggtacgctgg tggcggcttt gatcgtcaaa
     3781 ctgttgcaac acttcgcgga ctttctccgg cagcgtttca ccgtagccaa accaacgcag
     3841 cttctctacc agcaggcagg ttaacaggcc acaaatcaga accggaacgg tcaccggcga
     3901 catgcgcagg aagaaatcgc caaaatgcca gccagccgct ttagcgatga tcaggttctg
     3961 tggttcgccc accatggtca ttacgccgcc taatgcggtg ccgacaccgg catgcatcat
     4021 caggctacgc agaaagccac ggaactgttc cagaaccact ttgtaatgct tgtcgatatg
     4081 actatcgtct tgcaggtcgg tatcttcagt acgggaagag gctacgcggt gataaatacc
     4141 ataaaaaccg actgcaacgc tgatcaccac cgccacgacg gttaaggcat cgaggaacgc
     4201 ggagaggaac gccgccgcca cgcaaaaaga gagcgacagc agcattttgg aacgaatgct
     4261 taacagcaaa cgggtaaata tgaacagcaa tagctgtttc ataaaataga tacccgccac
     4321 cataaacatc agtaacagca agacttcaag atttgccgcc acctcttcac ggacgtgttc
     4381 cgcgctggtc atgccgatga ataccgcttc aatagccaac agaccaccgg ggagtagcgg
     4441 gtagcatttc agggccatcg ccagagtgaa aataaattcc gctaccagca accagccagc
     4501 gacgaaaggg ctgatgagga aaattaacgg atttacgatt aagaaaatga tgagggcgag
     4561 tttgtaccag tcgggggact ggcccaaaaa attgcgccat agcgcgcggc cccaggagat
     4621 ctccatgatg gtttccctta ccttacaaat aatcaatgat gtttttatgt ttaaacgcaa
     4681 agcttaacgg tcaggcagga gtgaggcaag tcttgatagt caaggggaaa gagatgcgga
     4741 aaatgaagcc ttgatccctt ttttcttctt tttgtctgct atcagcgtag ttagccctct
     4801 ggtatgatga gtccaacttt gttttgctgt gttatggaaa tctcactatg gtcattaagg
     4861 cgcaaagccc ggcgggtttc gcggaagagt acattattga aagtatctgg aataaccgct
     4921 tccctcccgg gactattttg cccgcagaac gtgaactttc agaattaatt ggcgtaacgc
     4981 gtactacgtt acgtgaagtg ttacagcgtc tggcacgaga tggctggttg accattcaac
     5041 atggcaagcc gacgaaggtg aataatttct gggaaacctc tggtttaaat atccttgaaa
     5101 cactggcgcg actggatcac gaaagtgtgc cgcagcttat tgataatttg ctgtcggtgc
     5161 gtaccaatat ttccactatt tttattcgca ccgcgtttcg tcagcatccc gataaagcgc
     5221 aggaagtgct ggctaccgct aatgaagtgg ccgatcacgc cgatgccttt gccgagctgg
     5281 attacaacat attccgcggc ctggcgtttg cttccggcaa cccgatttac ggtctgatcc
     5341 ttaacgggat gaaagggctg tatacgcgca ttggtcgtca ctatttcgcc aatccggaag
     5401 cgcgcagtct ggcgctgggc ttctaccaca aactgtcggc gttgtgcagt gaaggcgcgc
     5461 acgatcaggt gtacgaaaca gtgcgtcgct atgggcatga gagtggcgag atttggcacc
     5521 ggatgcagaa aaatctgccg ggtgatttag ccattcaggg gcgataatcc cttccgttta
     5581 aaaagcaaac ccctcaaacg aggggttttt tgttgttttt acagatttcc cattcttggc
     5641 gggcaacgtt ccagcaactc gacgctgcca tcttcgtttt gctgttccag catcacatca
     5701 aatccccaca ggcgatgcac atgcttcagc acttctttgc gcccccgatc cagcggtgcg
     5761 cgattatgtg gaatgtaacg cagcgtcagc gaacggtcgc cgcgcaaatc cacgttccag
     5821 atctgaatat tcggctccag attacttaag ttatattgcg acgataaccg gttacggatc
     5881 tcccgataac cttcttcatt atgaatagcg gaaatctcca gataattatg ccgatcgtca
     5941 tccagcacgg tgaagaagcg gaaatcacgc atcactttcg gtgacagaaa ctggctgata
     6001 aaactctcat ctttgaaatc acgcatcgcg aaatgcagcg tttccagcca gtcggaaccg
     6061 gcgatatccg ggaaccagta tttgtcttct tccgttggcg actgacaaat ccgtttaata
     6121 tcctggaaca tggcgaaccc gagggcatac gggttgatgc cgctgtacca cgggctgtta
     6181 taggggggct ggaagaccac attggtgtgg ctgtgcaaaa actccagcat aaaacgttcc
     6241 gttactttcc cttcatcata cagatggtta aggatggtgt agtgccagaa ggtggcccag
     6301 ccttcgttca tcacctgagt ctgtttttgc ggataaaaat actggctcac cttacgcaca
     6361 atacgcagga tttcacgctg ccatgattcc agcagtggcg catttttctc cataaaatag
     6421 agcaggtttt cttgtggttc ggacggatag cggcgcgctt cagcaaccgt tttctcttcc
     6481 tcgcgcttcg gcagggtacg ccagagcata ttgacctgac tttgtagata ctcttcgcga
     6541 cttttctgcc gggctttctc ttcttgcagc gagatttttt gcgggcgttt gtaccggtcc
     6601 acgccgtagt tcatcagcgc gtggcacgag tccagaagcc gttctacttc atccacgcca
     6661 taacgttctt cgcactcggt aatatattta cgggcaaaaa tcagataatc gacaatcgaa
     6721 ctggcgtcgg tccaactgcg gaataagtaa ttgtttttaa agaaagagtt atgcccatag
     6781 caggcgtgtg ccatcaccag cgcttgcatg gtaatggtgt tctcttccat caggtaagcg
     6841 atacacgggt tagagttaat gacgatttca taggccagtc cttgctgacc gtgcttatac
     6901 agccgttcag tctcgataaa ctttttaccg aatgaccagt gcggatagtt aattggcatg
     6961 ccgacgctgg agtaggcatc catcatctgt tctgaggtta tcacttcaat ctggtgcggg
     7021 taggtatcca gccggtagag tttcgccacc cggtctatct ctgccagata aacatccagc
     7081 aggtcgaacg tccagtcggg tccatcgctc aaccgtgtgg tgtccttatt catagaatcg
     7141 atcgtcgcca tacgcgcacc tcattgttgt cggcgctctc tgtgtggagc acctcatttc
     7201 aagcatagaa cacctgttaa aaaccgcgtc gccggagaat ttttttcttt gcgatttctt
     7261 attatcagag tgccactaat ccgcttctga acggaatttt atgctggata aaaagggcgt
     7321 tcagcaggag atactaaaga cgccatattg ccgcagagtc agggagatgt gagccagctc
     7381 accataaaaa agctgtatgt tgaataatat tttcaactga gttatcaaga tgtaattaga
     7441 ttattattct tttactgtat ctaccgttat cggagtggct atgcgagttg tcatactggg
     7501 aagtggtgtg gtaggcgttg ccagcgcctg gtacttaaat caggcaggac atgaggtcac
     7561 cgtcattgat cgggagccgg gggccgccct ggaaaccagc gccgcgaatg ccgggcaaat
     7621 ctcccccgga tatgctgcac cgtgggcggc accaggtgtg cctttaaaag cgatcaaatg
     7681 gatgttccag cgccatgcgc cgctggcggt tcgtctcgac ggtacgcagt ttcagttgaa
     7741 atggatgtgg caaatgttac gtaactgcga caccagccac tacatggaaa acaaagggcg
     7801 gatggtgcgt ctggcggaat acagccgtga ttgcctgaaa gcattgcgcg ccgaaaccaa
     7861 tattcagtat gaagggcgtc agggtgggac gctgcaactg ttccgtaccg aacaacagta
     7921 tgaaaatgcg acccgcgata tcgccgtgct ggaagatgcc ggcgtaccgt atcagctgct
     7981 ggaatccagc cgcctggcgg aagtggagcc agcgctggca gaagtagcgc acaaactgac
     8041 gggtggtctg caactgccca atgatgaaac cggagactgc cagctgttta cccagaatct
     8101 ggcgcggatg gcagagcagg cgggggttaa attccgcttt aatacgcccg ttgaccaact
     8161 gctttgcgac ggcgagcaaa tctacggcgt gaagtgtggc gatgaagtga ttaaggccga
     8221 tgcgtatgtg atggcgtttg attcttactc gacggcgatg ctcaaaggca ttgttgatat
     8281 tccggtgtat ccgctgaaag gctactcgct gactattcca attgcgcagg aagatggtgc
     8341 gccagtatcc accattcttg atgaaaccta caaaatcgcc attacccgtt tcgataaccg
     8401 cattcgtgtt ggcggaatgg cggaaattgt tggttttaac accgagctgt tgcaaccgcg
     8461 tcgtgaaacg ctggaaatgg tggttcgcga tctctatcca cgcggcggtc atgtcgagca
     8521 ggcgactttc tggacgggtt tgcgcccgat gacgccagac ggtacgccgg ttgtcgggcg
     8581 tacacgcttt aaaaatctgt ggctgaatac cggtcacggc acgctcggct ggacgatggc
     8641 ttgcggttcc ggtcagttgt taagcgatct gctctctggt cgcacgccag cgatcccata
     8701 tgaggatcta agcgtagcgc gctacagccg tggatttacg ccatcacgtc cgggccattt
     8761 acatggcgca cacagctaag gaaacgagat gacccgtccg atacaggcca gcctcgatct
     8821 gcaggcatta aaacagaatc tgtccattgt ccgccaggcc gcgccgcacg cgggtggtaa
     8881 aagcgaacgc ttacgggcat ggtattgagc gtatctggag cgcgctcggg gccaccgatg
     8941 gctttgcatt acttaacctg gaagaggcaa taacgttacg tgagcgcggc tggaaggggc
     9001 cgatcctgat gctggaagga tttttccatg ctcaggatct ggagatttat gaccagcacc
     9061 gcctgaccac ctgcgtacac agcaactggc agctcaaagc actgcaaaat gcgcggctaa
     9121 aagcaccgtt ggatatttat cttaaagtga acagtgggat gaatcggttg ggcttccagc
     9181 ccgatcgcgt gcttaccgtc tggcagcagt tgcgggcaat ggcgaatgtt ggcgagatga
     9241 ccctgatgtc gcattttgcc gaagcggaac atcctgatgg aatttccagc gcgatggcgc
     9301 gtattgagca ggcggcggaa gggctggagt gtcggcgttc gttgtccaat tcggcggcga
     9361 ctctgtggca cccggaagcg cattttgact gggttcggcc tggcattatt ttgtatggcg
     9421 cttcgccgtc cggtcagtgg cgtgatatcg ccaataccgg attacgtccg gtgatgacgc
     9481 taagcagtga gattattggt gtccagacgc taaaagcggg tgagcgtgtg ggctacggcg
     9541 gtcgctatac tgcgcgcgat gaacagcgaa tcggcattgt cgccgcaggg tacgccgacg
     9601 gttatccgcg ccacgcgcct accggtaccc ctgttttagt ggacggcgtg cgcaccatga
     9661 cggtggggac cgtctcgatg gatatgctgg cggtcgattt aacgccttgc ccgcaggcgg
     9721 gtattggtac gccggttgag ctgtggggca aggagatcaa aattgatgat gtcgccgccg
     9781 ctgccggaac ggtgggctat gagttgatgt gcgcgctggc gctacgcgtc ccggttgtga
     9841 cggtgtaact tgttgtaagc cggatcggag gcaacgtctt ctgggtgcaa aaaaaatcat
     9901 ccatccggct ggtcagcaac tgtagttgtt aatgtgacag agccattgcc catgatagtg
     9961 tccattaaaa ggatggacac tatttccccg gaacctgaac tcaccgcaca ggcgttctac
    10021 ataaaacgct tacgcttcat tgttgactcg gagtcgactt cagataaatc acgctttacc
    10081 cttgatggag cctgtacata gatttgtgta attgcctgat tttgatatgt tcaattcaac
    10141 atcaaatgaa ggttaaatta tggacgacaa acaattgcag gctcaggctg cgttcagcaa
    10201 agcatcgcaa ccggcgatag atgcttcatt aaatttaaga ttcagcttcc tcttcagcca
    10261 cccgtacgcc aatcttcaac acttcattat cttctttctc ggccaccgtc cagatcatcc
    10321 cggcaaactc tacctggtca ccgacaaccg gtgccgtgcc taacaactgc tggacaattt
    10381 cacccagcgt ttgctgctta tcacgatatt ctcgcccgtc ttccaggcca tatatcagcg
    10441 caacatcagc atatttggcg ctggcttcga gaatgaagtc accaaagaag cgttgatcca
    10501 gcgcgaccgg cggcgactgg ctgaacagtt taccgagcgc cggaagatcg cgttcccgac
    10561 caattacaca caacacatcg ccttcgcgca gtcgggtgct gccggtagga tgaagcaact
    10621 ggttatcacg aaacagtgcc gcaatacgcg tctcttttgg catatgcaaa tcgcgcagtg
    10681 ccgcgcccac gcaccattta tcggcactca attgataaac aaactgctcc cacggatttt
    10741 ccggatgaat atccaggcca acgcgtgaca ccggacgtcc cactggcgga accaccactt
    10801 tggctttttt agccgcccac gagagtgatg ttccctgcaa gagcagtgaa accagaacca
    10861 caaagaaggc gacattaaag aacagacgtg cattctccag ccccgccatc atcgggaaca
    10921 cggccaggat gattggcacc gcgccgcgta atcctaccca gctgataaac acgcgctcac
    10981 gcagattgaa accgcggaag gggagcaatc cagcaaatac cgaaagggga cgggcgaaga
    11041 atatcatcca tgccgacaaa attagcgccg gaatggcaat aggcagcaga tcgcttgggt
    11101 taaccaatag ccccagcacc aggaacatgg cgatttgcgc cagccaggcg aggccgtcga
    11161 aattttgcag gatgccgtag cggttgcgaa tcggccgatt acccagcaga aaaccgcaca
    11221 gataaacagc cagaataccg ctgccttcca gcgccgtggt taaagcaaaa attaaaatac
    11281 cgccgcttaa tgccagcaat ggatataatc cggcaggcag ggcgatgcga ttaatcattt
    11341 gcagcagtaa ataaccaccg ccaagtccga tgacaatccc gaggccaaat tgctgcagaa
    11401 tatcgaccac gaacatccaa ctgacactgc tttcatgttg ctggatcatc gcaatcaggg
    11461 taatcgtcag aaagaccgcc attggatcat tactgccgga ttcaatttcc agcgtcgagc
    11521 caacacgttc gttaagcccc ttaccaccca gcagagaaaa gaccgctgca gcatcggtag
    11581 agccgacgat agcgccgatt aataagcctt caatcaaatc aagattaaac agccacgccg
    11641 ccatcatgcc ggttaaacca gaggtgataa gcacgcccag cgtcgccagc gacagtgccg
    11701 gtcctaacgc cacacgaaag gaactggcct gagtgcgcat cccgccgtcg agcaaaataa
    11761 tcgccagtgc caggttactt accatgtagg cgaaggggta attatcaaac gggataccgc
    11821 cgacgccatc gactcctgcc agcatgccga tcgccagaaa aataaccaga ataggaatgc
    11881 caagacggga agaaaatgaa ctaagtaata tactgctggt gacaaggatg gatcctaaga
    11941 tgaaaaggct aattattgtt gtggcatcca acggtcggtt actcctgatt acgctgtctc
    12001 ttatataaac cctaccatat tagcggcaga gacagcgttt tacttagccc tgaagcgtga
    12061 tttttttaca ttttaagaac aggatgaccg ctaatcgtca actgagtgcc ttcccgggtg
    12121 ttattcagaa tggcatgtgc gcccaacggc agcgtaaccg tgcgttgttc atgaccaaaa
    12181 tcgaggccgg taatgagtgg aatcgacagg cgggaacgca aaaacgcgga cactgactcc
    12241 aggttgtaac ctgcgtcata atcattgggc gtgctgccgc taaagctacc gagaataatc
    12301 gccttctgac gtggcaaaat tcccgcatga tagagctgca acagcatacg ttcgacgcgg
    12361 aaagggtgct cgttaatatc ttccagtacc aggataccgt tctcaatttt tggcatccac
    12421 ggtgtaccaa tcagtgaaat cagcatcgca agattgcctc cccacaacgt gccttcggtc
    12481 tgacatgtcg ggccttcccc ttgccattca atggtgaagg ttttattgcg taacgccagc
    12541 cagaaatggt gctcggtaaa ggcgttcagt tcatccgcac caaagtttgc taccagcatg
    12601 gggccgctaa aggtgatgac attgccttgc gccagaagac cgcactgaat ggcggtaaaa
    12661 tcgctatgtc cgcaaatgag taacgggtca tgttgttggc gagccaccag tgcctgccag
    12721 tcaatatctg ccagtaaacg actggcaccg taaccgccgc gtacagccag cacgatggtg
    12781 ttgggtgtag tcagtctggc aagggaattg agatcttcca gacgctccgt ttccgtaccc
    12841 gcaaaacgct cacaacgacg ggcaataacc tcgacgttat tgacttgatg ccccgcgtcg
    12901 gtaaggcgct ggataccacg cagcgcggcg tgctgtttaa tgctgtaacc cgatggggca
    12961 attaagtgaa acagagacat ggcaattcct tgctgacaac agaaacgaaa tgtatatcat
    13021 gccgcttagg tgtgccgttg tcacctcaac ggcgattcca ggctataagg atagaagaag
    13081 tgaaattgag atggtttgcc tttttgattg tgttattagc gggttgttca tcaaagcatg
    13141 actatacgaa cccgccgtgg aacgcgaaag ttccggtgca acgtgcgatg cagtggatgc
    13201 caataagcca gaaagccggt gcagcctggg gcgtcgatcc acaattgatc acggcgatta
    13261 tcgctatcga atcgggtggt aatccgaacg cggtgagtaa atcgaatgcc attggtttga
    13321 tgcagttaaa agcttcaacc tccggacgtg atgtttatcg ccgtatgggc tggagtggtg
    13381 agccgacgac cagcgagctg aaaaatccag agcgtaatat ttcaatgggg gcggcttacc
    13441 tgaatattct ggaaaccggc ccgctggcag gcattgaaga tccgaaggta ctgcaatatg
    13501 cgctggtggt gtcatacgct aacggggcag gtgcgctgct acggactttc tcgtcagatc
    13561 ggaaaaaggc gatcagcaaa atcaacgatt tggatgctga cgagttcctc gatcacgtag
    13621 cgcgcaatca tcctgcgccg caggctccgc gctatatcta caaacttgag caggcactgg
    13681 acgcgatgta aatcagtcgc gtactttgtc cgctttctcc cgggcttccc gctcgagaga
    13741 gaaaataatc cgttgtaatt gccgctccac cgtcgggctg acgttaagaa aacggaagct
    13801 cagacgggga gtggtgatgg tttcattctt gccatcaatc actttgcgct cgctgatgga
    13861 gattaactgg gcgtcaaagt gaaaaacacc ccattgcccc atgttgactt caatctgagc
    13921 gaagcgcatg ccttcttgta attcggcagg ctttgctgtt tccagtaatg cgcccatgcc
    13981 gcctaacgac aaatcataca ggcggaaacg taacgtactg ttatccgcca gtttggtctg
    14041 gcaaaaataa ggcggatgga gtggggcgga gatgcggaaa tatcggcgtc gttgtacaaa
    14101 ccataaggtg ggaggcggta cggtaataaa tgccggaagc tgcaagtatt cactctgctg
    14161 tagttgttca acagtaaact cgactttcgc accctgagtt tcggcggtaa tggtaatatg
    14221 ctgcgccttt agcacggcga tgttgtcttc ggcttgactg ccgaaatcca gcaccagttt
    14281 atccggggtt attgccagta ttttgctgat cagctgcccg ccattccaac tgagacgcaa
    14341 aggaattgcg gctttgtgca aatcgcgtaa cacgcccagg acggctaacg gattttgttt
    14401 caggaactgc tcatggtaat gactcacgcg gaaaaactcc tgactgacaa actgtctttg
    14461 gtttatcggt cacagttaac aaaacttaat acaaatgcgt gaatattttt tacatgttgt
    14521 tcttaaatca gccgacatac gcccagcatc gatcccgccc ttgcctatac ttagagcgtt
    14581 gacgtaagca tttcttgcgt ccgattcatc gaaacgaggg cttgaacatg ggaattattg
    14641 cctggattat ttttggcctg atagccggca ttatcgccaa gctaatcatg ccggggcgtg
    14701 atggtggtgg atttttccag acctgtattc tcgggatagt cggtgcggtg gtcggcggct
    14761 ggctggcgac catgtttggc attggcggct ccatcagcgg ttttaatttg cacagcttcc
    14821 tggtggcggt ggtgggagct attctcgttc tgggcttatt ccgcctgctg cgaagagaat
    14881 aagattttca taaggcggat agcgatacag atgccgctat ccgcttttca catcagaacg
    14941 tatattcgac acctgcttta cgggtgaaaa aaatcaatat agcacttcga tatccgataa
    15001 agtcggttcc cggtaacaac aacagaatcc ttttttaatt attgtttcgt tgtttttggt
    15061 gtgattcaga aaataaatat tttagacagt agccagtcag gcgttaaaaa tggggcggat
    15121 agtgaggcgg ttttggctgc gcttattgaa catttagaat tgatcaatcc ttctggcaga
    15181 ttaagccaaa atacccgttc ggcaatgctt cagttacgcg aagaggaatg gtctgaattc
    15241 tttttttggt tgctaaattc tctggagtgt ctggattatg tgattattaa tttaactcca
    15301 gaaagcaaaa aaacgttgat gagtgaacac cgtaacaata ttcaggtagc aatcgatgct
    15361 ttatataggc aacgtcggtg taaatcaccg ggggatgata gtgaaacact tacccgtaga
    15421 aatgacgcta tcttcggcaa ccacgtatgg caaacatttg cgcaatactt cccgccagga
    15481 cttgaaaaac cggctgtgta gaaaacacca tagctatggt gttatctgcc gacaacgcca
    15541 tccggagaag ccgaatgacg ttgtcggagt aagcgctggt taaggtgtgg gttgtgcctc
    15601 tttggttgag ggttgcgtcg ttgctgactt aacggtcgga cgcgtcgccg gaacattgtc
    15661 acacggttgc tctttcgggc agatcaaatc cagcattttc agcgtcacgc cattggtcca
    15721 gccaaagcca tcctgtaatg gatattcgcc acctcttttt gcccgtagtt ttgtaatcct
    15781 tctgtcgcga cccactgtaa cggtgcccag ccatttggcg catcccattg ttgcccactt
    15841 ttcaccgacg tggtgttcag gccgccgggt tgcagcagat gtgttttcgt cgccgtcgcc
    15901 attttgttgg cgcgatcttt cgctgccgca ttgacgtaca gcgggaacag ggcggccgcg
    15961 gttaactgat tgcgcacttt atgacttttc aggtcgtaat cggcatacca gccttgttga
    16021 tcgttccaca ggtatttttc gattcctttt tgacgggcat tcgccagcgt ttcgtactgg
    16081 tttgccatcg cgttatctcc tgcagctttg ctggcgcggg cgaggatttt ttccatttta
    16141 aacatcaggc tgttcagatc gaccggtacg atgctggtgg tgcgtaaggt atttaactgc
    16201 tgcgggttgt ccatccagcg cgagctgaaa tcccagccag acgcagcggc agagcgcagg
    16261 tcgcggtaaa tttcagtggc aggtcgattc ggattgcttt tggcggtggc aatatcttcc
    16321 acccatgact ctggtcgtgg cgtatcgcga tcgtcccagt agcggttgag aagggtacca
    16381 tcctgaagtt tgacaacgcg tttttcctgt tgtccggctt gcaggttttc aacaccgtcc
    16441 atccagtaag catattcttt ttgcatttgc ggcaggtatt gcttcaacgc ggcatcgcct
    16501 tcatgctgcg ccagtaactc taccatcagg gcaaagaagg gcggttgcga gcggcttaaa
    16561 tagtaactgc ggttgccgtt gggaatatga ccgtaagtgt ctatttcatg agcaaaattg
    16621 gccaccatat ccgcgacttt atcccagtga ccgctttcgg caagtcctaa catggtgaag
    16681 taactgtccc agtaatatac ctcgcgaaaa cgtccgcccg gcacgacata aggttccggc
    16741 agcggtaaca gagaatccca tttttcggtg ttttcggtag aacgcgttaa taccggccaa
    16801 agtccgtcaa tatgttcgcg cagtgactgc ccctctggcg gaacatattt ctcgccttct
    16861 ttcggcaggg tgaaattgac gttaacgaaa tggcgcagat caaatccgct ctggttttgc
    16921 tgcatccgat aatcagcgga tcgctgttcg gcacggcatc ggcaaaggtt ttttggtccg
    16981 gaaaaagttt ggcgttttgc acatcattaa acagc
//
LOCUS       DAJVUJ010000089        16622 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.089, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000089 DAJVUJ010000000
VERSION     DAJVUJ010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16622
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.089"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(<1..245)
                     /locus_tag="OQQ48_002576"
     CDS             complement(<1..245)
                     /locus_tag="OQQ48_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCS1953300.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPIVTWIV"
     gene            352..648
                     /locus_tag="OQQ48_002577"
     CDS             352..648
                     /locus_tag="OQQ48_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS1953301.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            complement(782..1207)
                     /gene="arsC"
                     /locus_tag="OQQ48_002578"
     CDS             complement(782..1207)
                     /gene="arsC"
                     /locus_tag="OQQ48_002578"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HCS1953302.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(1220..2509)
                     /gene="arsB"
                     /locus_tag="OQQ48_002579"
     CDS             complement(1220..2509)
                     /gene="arsB"
                     /locus_tag="OQQ48_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HCS1953303.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(2564..2917)
                     /gene="arsR"
                     /locus_tag="OQQ48_002580"
     CDS             complement(2564..2917)
                     /gene="arsR"
                     /locus_tag="OQQ48_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HCS1953304.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKIQAIVRN
                     LARQNCSADSKNICS"
     gene            3656..3739
                     /locus_tag="OQQ48_002581"
     CDS             3656..3739
                     /locus_tag="OQQ48_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HCS1953305.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(3793..5145)
                     /gene="gorA"
                     /locus_tag="OQQ48_002582"
     CDS             complement(3793..5145)
                     /gene="gorA"
                     /locus_tag="OQQ48_002582"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HCS1953306.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKMLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSEHLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGSEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(5217..6059)
                     /gene="rlmJ"
                     /locus_tag="OQQ48_002583"
     CDS             complement(5217..6059)
                     /gene="rlmJ"
                     /locus_tag="OQQ48_002583"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HCS1953307.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            6262..8304
                     /gene="prlC"
                     /locus_tag="OQQ48_002584"
     CDS             6262..8304
                     /gene="prlC"
                     /locus_tag="OQQ48_002584"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HCS1953308.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTQALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            8312..9064
                     /gene="rsmJ"
                     /locus_tag="OQQ48_002585"
     CDS             8312..9064
                     /gene="rsmJ"
                     /locus_tag="OQQ48_002585"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HCS1953309.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVTTPNAVVTKGHRFDIYAGTPV"
     gene            complement(9113..10582)
                     /gene="dtpB"
                     /locus_tag="OQQ48_002586"
     CDS             complement(9113..10582)
                     /gene="dtpB"
                     /locus_tag="OQQ48_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HCS1953310.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFKPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINSVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(10900..11334)
                     /gene="uspA"
                     /locus_tag="OQQ48_002587"
     CDS             complement(10900..11334)
                     /gene="uspA"
                     /locus_tag="OQQ48_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HCS1953311.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            11725..12060
                     /gene="uspB"
                     /locus_tag="OQQ48_002588"
     CDS             11725..12060
                     /gene="uspB"
                     /locus_tag="OQQ48_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HCS1953312.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(12304..13803)
                     /gene="pitA"
                     /locus_tag="OQQ48_002589"
     CDS             complement(12304..13803)
                     /gene="pitA"
                     /locus_tag="OQQ48_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HCS1953313.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKIPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            14035..15237
                     /locus_tag="OQQ48_002590"
     CDS             14035..15237
                     /locus_tag="OQQ48_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCS1953314.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
     gene            complement(15552..16604)
                     /locus_tag="OQQ48_002591"
     CDS             complement(15552..16604)
                     /locus_tag="OQQ48_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HCS1953315.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNILFPIIGYAGSIITMIWGWALLAGNDVMA
                     DEFVAGYVIFGIGMIAACVSTVAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSADITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTF
                     STKEHWLWCYWVIFLGSITVLQGIYVLVSSDASARLAPGIILIYLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
BASE COUNT         4270 a         4238 c         4314 g         3800 t
ORIGIN      
        1 acaatccacg taacaatggg tattgtcgag caactacaga acggggtaac ggctcccaac
       61 agagcagcca ggagatagcc tctccctttt ctggccccca tcatctgctg gattttatgg
      121 tctggcacct tttgtcttat caggctgaca ccggcactaa tcactataaa cagcaacgaa
      181 agctcgacgg cgagaaacac aaacatctct gcggcatctt gcagcatcgc aagccaacta
      241 ctcatacgta acctcattgg ataaatctat atttccagaa taatcgaaat gaaattcaga
      301 tcaagatatt tctggtatta tcgaaatatc aaccagacag aggggtgtca tatgcagttg
      361 gaagaagtcg ctaaagcgct gaaagagcta ggtcacccga cccgtttgtt catatttaaa
      421 catcttgtca aagccgggga acaagggttg ccggtcggtg agttgcaaaa gcagcttggg
      481 atacctggct ccacactcag ccaccatata tcagcactgg tatcggtagg actggtcaaa
      541 caaaaccgtg agagtcggac gctgatgtgt gtttcgcaat acgcaatgct ggaagcgata
      601 attgaattct tacgcgagga gtgctgcgta aatagcaaaa ttgcctgatg cgctacgctc
      661 tacatatttc atgccatata ttgaatttgc acggttttgt aggtcggata agacgcgtta
      721 gcgtcgcatc cgacataaac aaagcgcact ttaccaacta tcggcagtgg atatcgccgc
      781 tttatttcag gcgcttaccc gcttcatcaa ccactttctc gccatcttcc ttggagaatg
      841 cgcctttttg cgcatctggc agaatttcca gcaccacttc tgaagggcgg cacaggcgag
      901 ttcccagcgg cgtcaccaca atcgggcgat taatcagaat cgggtgctga agcataaagt
      961 cgattaaccg atcgtcagta aatttatctt ccgcaaggcc cagctcctca tacggttcga
     1021 cgtttttacg cagcagcgcg cgtacggaaa tccccatatc ggcaatgagt ttgaccagtt
     1081 catcgcgcgt tggcggagtt tccagataat ggataatggt cggttctgtg ccgctgttgc
     1141 ggatcatctc cagcgtatta cgcgacgtgc cgcaggccgg gttgtgataa atggtaatgt
     1201 tgctcatatc agtatctcat tacaaagtga aagagagacg tagcgccagc gcagccagcg
     1261 tcacaaacag cacaggcagg gtcatgataa tccctgtacg gaagtaatat ccccagctga
     1321 tagtcatatt cttctgcgaa agtacgtgca gccagagtag tgtagccagg ctaccaattg
     1381 gggtaatttt cggtcccaaa tcgcagccaa tcacattggc ataaaccatc gcttctttga
     1441 taacgccaga tgccgtgctg ccatcaatgg acaacgcgcc aaccagtacc gtcggcatat
     1501 tgttcataat agaagagagg aaggcggtga ggaatccggt gccgagcgtc gcggcccaca
     1561 ggccgttatc cgccagcacg ttgagtacgc cagaaagata ttccgttaat ccggcattgc
     1621 gcaggccata aaccaccaga tacatgccga gcgagaagat gacaatttgc cagggggcac
     1681 cgcgcaggac tttacccgta ttaatcgcat gaccgcgttt agcgacgaca aataatatca
     1741 gcgcgcccac agctgcaatg gcgctcaccg gaatgccgag cggttccagg acgaaaaatc
     1801 ccaccagcag aagcagtaaa acaacccagc cagttttgaa cgtagcagga tctttgatcg
     1861 cttctgcggg agatttcagc agcgccatat cgtagttctg cggaatatct ttgcgaaaat
     1921 agagatgtag catcaccagc gtggcaacaa tcgcggcgat atccaccggc accatcaccg
     1981 aggcgtattc gcgaaagccg aggccaaaga aatcagcgga aacgatattc accaggttgg
     2041 agacaataag cggcaggctg gcggtatcgg caatgaatcc ggccgccatc acgaacgcca
     2101 gcgtcgtgcc tttactgaat cctaaagcca gcagcatggc gatgacaatc ggcgtcaaaa
     2161 taagcgctgc gccatcattg gcaaacaggg cggcaacggc agcaccgagc aggacaatcc
     2221 aggtaaacag caagcgacca cgaccattac cccagcgtga gacgtgcagc gccgcccatt
     2281 caaaaaagcc ggactcatcc agcagcaggc tgatgataat gacggcgata aacgcagccg
     2341 tcgcgttcca gacgatattc cacaccaccg gaatatcacc cggatggacc acgcccgtaa
     2401 ctaacgccag tactgcgccg agcgttgcac tccagccgat gcctaaacct ttcggctgcc
     2461 agataaccaa tacaatggtc aggacaaaga tagcgcctgc cagtaacata atgcctcctg
     2521 gataaaacac atgcgaaaat acatatgtgt ttagctaaat tttttaactg caaatgttct
     2581 tactgtctgc ggaacagttt tgtcgagcca ggttgcggac aattgcctga atcttttcct
     2641 gttcacatcg ccaggcttca tcaattattt tcgccgccca tgctggaata tgcggcgata
     2701 agcggtaatg aacccattta ccttgcttgc ggtccagcaa tagcccgctt tcacgcagca
     2761 atgccaggtg gcgggagatc ttgggctgcg actggtcgag agcagtgcag agatcgcaga
     2821 cgcataactc tcccagttcg ctgagcagta aaacgatgcc cagacgggtt tcatcagcaa
     2881 gaattttgaa caattggatg ggtaacagaa atgacatatt gcgctcctga ttgttgcagg
     2941 tagtgtctct cttcgaagcg gataagtcaa aaacatatat gatttaacga atgtgtaagt
     3001 gcagaggaag gtaataggtg tgaattttga gttggctatt catttgaaag gaggtctgaa
     3061 tcaggacgta aaaaaaccga ctttgcgtcg gttttttact ttccagccct gagttggtgg
     3121 ctctggctgg agtgagagag ttcttatcta acagctcaat actattagcc agtggctaac
     3181 attgcaagaa ttagcgattt cttcagctgg cggagggagc acttcggtat atatcgcagt
     3241 gacgatttat atactttcac tgggtcatcg tcatattaag ccttgtccga atccggatgt
     3301 aaaaaaccga ctttgcgtcg gttttttact ttccagccct gagttggtgg ctctgactgg
     3361 agtgagatag ccatcatcta acgcatcaat gctaatacca tactgaaact attgcaagga
     3421 cgtgctggtt ttataacctg catgtactgt atgattatcc agttagctct gaggcatttt
     3481 cactctggca atgcgcataa acgctttcaa agtcctggtc agaagtacgg gtggtgccgt
     3541 taactgatgc tctggccgga gtgagagagt tcttatctaa caatgagaca tgcgccgtga
     3601 caggcagtgg atgagtaagc ggatgcattc tcactccatc gcatggagaa aacgggtgat
     3661 tgataaagca atcatcgttc taggggcgtt aattgcgctg ctggaactga tccgctttct
     3721 gcttcagctt ctgaactgat agcggaaacg taattaaggg ctaagagcac actactctta
     3781 gccctttaac atttaacgca ttgtcacgaa ctcttctgcc gccgttgggt gaatggcgac
     3841 ggtattgtcg aagtcttttt tggttgcccc catcttcagc gccaccgcga agccctgcaa
     3901 catttcgtcc ataccaaagc caatgccgtg aataccgaca atcttctctt ccgatccaac
     3961 gcacaccagc ttcatgcggc acggctggcg gtgagtggtg acggcggtat acatcgcggt
     4021 gaaagaggat ttatacactt tcacctgatc gtcgccatac tgctcgcgcg cctgcggttc
     4081 cgttaaacca acagtaccaa tcggcggatg gctgaagacc acggtcggaa tgttgctgta
     4141 atccagatgc tcatccggct tgttattaaa caggtgttca gagagacggc gacccgctgc
     4201 aactgccacc ggtgtcagct ccactgcacc cgtgttatcg cccaccgcgt aaataccttc
     4261 aatgttggtg ttttgatatt tatcgacgac gatatagcct ttttcgttgg ttttaacgcc
     4321 agcggcttcg aggttgatgt tgtcattggc aggctcgcga ccaatcgccc aaatcaggca
     4381 atccaccgtt tcactgcgac catcttccag ctccagcgtc aggctaccat cggcattttt
     4441 cactaccgct ttcgggatgg cgttggtgtg cagctgcggg ccttcggcgt tcatcacttc
     4501 gaccagcgtt tcggaaatca tcgggtcgaa gctgcgcagc ggcgcatgtt tacgcacaaa
     4561 cagatgcgtt ttcgcgccga ggccgttaat cacgcccgcc agctcaacgg cgatgtaacc
     4621 cgcgccaaca accgccacgc gctctggcaa agcaggaagg gcgaagaagc catcagaatc
     4681 aataccgtat tccacgcccg gaatatccgg gtggctcgga cgaccgcctg tggcgatcag
     4741 aatatgatcg gccgtgatgg tttcgccgtt tacctccagc attttggcat caacgaagcg
     4801 ggcaaagcct ttgattacat caacgttatt tttaccgagc acgttttcat aggaagtatg
     4861 aatacggtcg atataggcgg tacggctggc gatcaacgtt tcccagttga acttgttgat
     4921 ggtggtatcg aaaccataat cagggccgta catatggatc gcttcacgga tttgcgccgc
     4981 gtgccacatc acttttttcg gcacgcagcc gacgttcacg caggtgccgc ccagctcttt
     5041 ggcttcaatc agtgcgcatt tctggccgta catagccgcg cggttgatgg aggctatacc
     5101 gccgctgccg ccgccgatgg cgatgtaatc atagtgttta gtcatgacag agtgtcctta
     5161 tcgttgatta ccgcgattgt agcgcgagac gtaataggtg ccagcaatgg ctgcaattac
     5221 tccggcacga tccagcttac ggtggcgtgc ccggtgcctg ccggaaccag tttgctgtgc
     5281 agccacggca gcacgttatt catctgctgt tccagtttcc acggcgggtt aatcacaatc
     5341 atgccggaag cggtcatgcc acggcgatcg ctgtctggca gtaccgccag ttcaatttgc
     5401 agaattttgc gaataccggt cgcttccaga tcgtggatca tgcgcttaat ttgctgacgc
     5461 agcaccaccg gataccacag tgcgtaagta ccagtggcga aacgtttgta accttctgct
     5521 atcccgctga ccaccgcctg atagtcggtt ttcatttcat acggcgggtc gataaggatt
     5581 aaaccacggc gggaaaccgg cggcagtttg gccttaagct gctggaaacc atcggctttt
     5641 tcgacgcgcg cacggctgtc tttctgaaat tcagaacgca gcaacgggta atcgctcggg
     5701 tgcagttcgg tcagttgcag gctgtcttgt tcgcgcagta gctggcgagc aatcaatggc
     5761 gaaccggggt agtaacgcaa ctgaccgcta cggttgaagt gttttaccac attgatgtac
     5821 gcctccagtt ctgcgggcaa atcgtcctgc tgccagatac gggcgatgcc ttcgagatat
     5881 tcgccggtac gctcggcatg ttcgctgcct aactgataac gcccggcccc tgcgtgggtg
     5941 tcgagataga gaaacggttt atctttctct ttcagcgact cgatgatcag gctctgaacg
     6001 gtatgtttaa ggacgtcggc gtggttgcca gcgtgaaagc tgtggcgata actgagcatg
     6061 ggtaaaggtg ttccggtaag taaaaatcgg cccgtattcg gggcgcacaa aagcgtatca
     6121 ggacagtata ccgaaaagag gccgccgccg cgaaagcgta acgtttctca ttgaaattca
     6181 ctacacttaa ccccatgcta cacacattat gtaaagcgcc tgttgagcgc ttccttaacc
     6241 tctttaacca ggactgcgcg aatgacgaat ccgttactga ctccctttga attgcctccg
     6301 ttttctaaaa ttctcccgga acatgtcgtt ccagccgtga ctcaggcgct gaacgattgc
     6361 cgcgaaaatg tggagcgcgt agtagcgcaa ggggcaccgt acacctggga aaatctctgc
     6421 cagccgttgg cggaagtgga cgatgtgttg gggcgtatct tctccccggt cagccacctg
     6481 aactcggtga aaaatagccc ggaactgcgt gaagcctacg agcaaaccct gccgctgctg
     6541 tcggaataca gcacctgggt agggcaacat gaagggctgt ataaagcgta ccgcgacctg
     6601 cgcgatggcg atcattacgc cacgctgaac acggcgcaga agaaagcggt tgataacgcc
     6661 ctgcgtgatt ttgaactctc tggcataggt ctgccgaaag agaaacagca gcgttacggc
     6721 gaaattgcga cccgtctttc tgaactgggc aaccagtaca gcaacaacgt cctcgatgcg
     6781 acaatgggct ggaccaaact cgttaccgac gaagcggagc tggcggggat gcctgaaagc
     6841 gcgctggctg cggcaaaagc ccaggccgaa gcgaaagagc tggaaggcta cctgctgacg
     6901 ctggatatcc caagctacct gccggtaatg acctactgcg acaaccaggc cttgcgtgaa
     6961 gagatgtatc gcgcttacag cacccgcgcc tccgatcaag gcccgaacgc cggtaaatgg
     7021 gataacagca aggtgatgga agagatcctc gctctgcgtc acgaactggc gcaactgctg
     7081 ggctttgaaa actacgcctt taaatccctt gccactaaaa tggcagaaaa cccgcagcag
     7141 gtgctggatt tcttaaccga tctggcaaaa cgcgcgcgtc cgcaaggcga aaaagagctg
     7201 gcacaactgc gcgccttcgc caaagcggaa tttggcgtcg atgagttgca gccgtgggat
     7261 atcgcgtact acagcgaaaa acaaaaacag cacctctaca gcatcagtga cgaacagctg
     7321 cgtccgtact tcccggaaaa caaagcggtt aacggcctgt ttgaagtggt gaaacgtatt
     7381 tacggcatca ccgctaaaga gcgtaaagat gttgatgtct ggcatccgga tgtacgtttc
     7441 ttcgaactgt atgacgagaa caacgaactg cgcggcagct tctacctcga tctgtatgcc
     7501 cgtgaaaaca agcgcggcgg ggcgtggatg gatgactgcg taggccagat gcgtaaagct
     7561 gacggttctc tgcaaaaacc ggtcgcgtat ttgacttgta acttcaaccg cccggtaaat
     7621 ggtaaaccgg cgctgttcac tcacgacgaa gtgatcaccc tgttccacga gttcggtcac
     7681 ggcctgcacc atatgctgac ccgcatcgaa accgctggtg tttccggtat cagcggtgtg
     7741 ccgtgggatg cggtcgaact gccgagtcag tttatggaaa actggtgctg ggagccggag
     7801 gcgctggcgt ttatctctgg tcactatgaa accggcgaac cgctgccgaa agagttgctg
     7861 gataaaatgc tggcggcgaa gaactaccag gcggcgctgt ttattctgcg ccagctggag
     7921 ttcggcctgt tcgatttccg cctccatgcc gagttccgcc cggatcaggg tgcgaaaatc
     7981 ctcgaaactc tggcagaaat caagaaactg gttgccgtag taccgtctcc atcctggggc
     8041 cgtttcccgc acgctttcag ccatattttc gccggtggtt atgccgcagg ttactacagc
     8101 tacctgtggg ctgacgtact ggcggcagat gccttctcgc gctttgagga agagggcatt
     8161 ttcaaccgtg aaaccgggca gtcgttcctc gacaacattc tgagccgtgg cggttcagaa
     8221 gagccgatgg atctgttcaa acgcttccgt ggtcgtgaac cgcagctgga tgcgatgctg
     8281 gagcattacg gcattaaggg ctgatcattc agtgaaaatc tgcttaattg atgaaacagg
     8341 cgccggagac ggcgccttat ctgttctggc ggcccgctgg gggctggagc acgatgaaga
     8401 caacctgatg gcgctggtgt taacgccgga acatctggaa ttgcgcaagc gtgatgagcc
     8461 aaaacttggc ggcatctttg ttgattttgt tggcggagcg atggcgcacc gacgcaaatt
     8521 cggcggtggt cgcggtgagg cggtggcgaa agcggtgggc attaaaggcg attatttgcc
     8581 ggatgtggtg gacgccaccg cagggctggg gcgcgatgcc tttgtactgg cttcagtcgg
     8641 ctgccgcgtg cggatgctgg agcgtaatcc agtggttgcc gcgctactcg acgacggcct
     8701 ggcgcgtggt tatgcggatg cggaaatcgg cggctggttg caggagcggt tgcagttaat
     8761 tcacgcctcc agcctgacgg cgctaaccga tattacccca cgcccgcagg tggtctatct
     8821 cgatccgatg ttcccgcata agcagaaaag cgcgctagtg aaaaaagaga tgcgtgtctt
     8881 tcagtcgctg gtgggaccgg atcttgatgc cgatggatta ctggagcctg cacgcttgct
     8941 ggcgaccaaa cgcgtggtgg taaagcgccc ggactacgcg ccaccgctag cgaatgtcac
     9001 cacgccaaac gcggttgtga ctaaagggca tcgctttgat atctatgcag gtacgcccgt
     9061 gtaaaagtaa agcagggtag cgataacgct accctgcttt ctgcaagaat aattaatggc
     9121 tttccggcgt cgcaatcatg cgtttcagcc acggcaccat caacagcatc actactgcaa
     9181 cgcccagcgt gaccagacca atcttaccaa acacgttggt atagacgggc aacgtctcaa
     9241 gcggatcggt aatgttgtcc ggcaccgcgg taaatgttgc cacatagccg cccagcaaga
     9301 acgcggcagc ctgcgtcagg aaccacatcc cgagaataaa gcccatcaaa tgctgcggca
     9361 ccagggcagc aatcatcgcc aggccaaggg cgctaataaa cagttcacct aagctctgga
     9421 ataagtacac cagcacgata aaccatggcg atgtcagccc ttgtgcatcc gcaaaccaca
     9481 ttcccgcagc tgccgccgtc aaaaagccca gtgagcacat aaacatgccg agagtaaatt
     9541 tcatcggcat cgagaggtct ttgcctttgt tacccagatg cgtgtaaatg cctgccagta
     9601 ttgggctggc gagtaccacc cagaacgggt taagcgcctg gaagctgacc gagttgatgg
     9661 aaaaaccgag aatttcatga tgcacgttgt tgatggcaaa gaagttcagc gatgttggca
     9721 tctgggcgta gagaatgtaa aacaccaccg cttcgagcat caggacaaag gcgacaaaca
     9781 ttttattgcg cccggtttta tccagcttga atgcctgacg aaagaagatg atggtgacga
     9841 cgatggagag aacaatcagc accagattgg cgacttctac gttgtgcatc agccatgcac
     9901 atacgaagat catcaccacg ctgccaagta atacgtacaa cagtttgctg aagctcatcg
     9961 gcttgaagtc gggttcagaa ccaatgtctt tcaccattcc acgacaggcg atgtaaacca
    10021 gtaatgcgat aattaacccc gccccgcaca ggttgtaggt gactgaataa ccgaatctat
    10081 cagcgatcac aggggccagc gataacgcta tcaacgagcc gatattgatc gacatataga
    10141 acagggtgaa tgcgccatca agccgcggat ctttcggcgg atagcacttc gaaagcaagc
    10201 tggctgggtt agctttaaac aggccgttac cgacagcgat agtccccagg gcgatgaaaa
    10261 tcaggtcagg cttaagtagc gacatgccgg tcatgaagta gccaatcgcc agcacaagtg
    10321 ctccgagaac aatggtgcgt ttggtcccca gcaggtggtc gccgacatag ccgccaatgg
    10381 aaatgaggcc atagaccagc gcagcaaaag caccaaaagt gacaaaagcc tgctcttgcg
    10441 agaatccaag ctgtttaacg aagaaaaccg ccagtacgcc ctgcacgccg tagtagccga
    10501 atcgctccca taactcgaca aaaaagatca tgaaaaatgg gcgaggttgc tgcagcatcc
    10561 ccatgggtgt tgttgtattc atatttatta accttccaat accatcctga aaagtatgaa
    10621 cgcgtaaggg ataacgcgcg aaaaagttta catctgtcag agattcttaa tattgatgcg
    10681 gtgaatggat taatacacta ttacaacaga aaataaccag ctaatcacac taatgatgga
    10741 aaatatgatg gtgttaaatg ttgtattggc aggagatttt gattgcgtac gcagatcgta
    10801 ggccagataa ggggtttacg ctgcatcagg taacaaggcg cgccaaaaag caaaacgccc
    10861 gcatatgcgg gcgttcagga acacgtcgtg gagggaagat tattcttctt cgtcgcgcag
    10921 cggaacaatc agcatatcaa cgtgaacggt gttgatcagc tgacgtgcgg aagacatcag
    10981 tttgctccag aagtcctggt ggtgaccaca aaccaccaga tccatatcgt atttcttgat
    11041 tgcatcgacc agaacctggc ccaggtcgcc gctgccgctc agggtttcag tgattgggta
    11101 gcctgcatta gtggaaagct cggtcagcgc atgatgtgtc tcttcagaga tgcgtttctg
    11161 catatcaccc agattcacat caataagccc ggtgtatagg tcagagtagt ttacatctac
    11221 gtggatcaga gaaactttcg cattgtaggg gcgagccata gagactgctt tctctaccag
    11281 aactttgctt tccggggaga gatcgaccgc gatgagaatg tgtttataag ccatagtgtt
    11341 actccttcca taaagttgtc gatgactggc cagctagcgt ttcttgtgcc gttacgtcac
    11401 ccgcgtcctg cgaactacgt gcgcggggct aacccagccg taaagattca gtgttaagac
    11461 catccttacc ttatagcgac ccggatgatc cgtcaatccg ccttgcttac ccgttagata
    11521 aacaaatttt cgacatgaat atattgatag tggttaacct tctggaaaaa aaacaacctg
    11581 atctcctaca ctatctatag agccgctcgg atgttggtca ccacaaaagc ggtttctcca
    11641 ggacctggtg ttaactgatt ggcagcgtat cgggagcggt agccccgggg tggaactccg
    11701 cgggcaggtc gccggggagg aggtatgata agcaccgtcg cattattttg ggctttatgt
    11761 gtcgtttgca ttgttaacat ggcgcgctat ttctcatcac tacgcgcgtt gttagtggta
    11821 ctgcgtaact gcgatccatt gctctatcaa tatgttgatg gagggggctt ttttacctca
    11881 catggccaac ccaacaaaca ggtgcgtctc gtttggtata tctatgccca acgttatcgc
    11941 gatcatcacg atgatgagtt tattcgccgc tgtgagcggg tgcgtcggca gtttattctg
    12001 actagcgcat tgtgtggtct ggtggtggtc agcctgattg cattgatgat ttggcattaa
    12061 gcacttcagc aataataacg cataaaaaaa gctggccagt aaactgaccc gctcgagact
    12121 gattacaaac gcaaaattgc ctgatgcgct acgcttatca ggcctgcata atctctgcaa
    12181 tctattgaat ttgcatgttt ttgtaggccg aataaggcgt tcacgccgca tccggcatga
    12241 acaaagcgca ctatgtcaca atctgaagcg ggccagctga cccgctcgtt ttggtgcgta
    12301 cgattacagg aactgcaagg agagccagta cagcccgccg gaaagcagta ccgcagccgg
    12361 aagggtaaac acccaggcca tcagaatgct ggtcacggtt ttacgctgta agccgccacc
    12421 atctaccacc atcgtccccg cgacagaaga ggagagtacg tgagtagtgg aaaccggcat
    12481 cccggtataa ctcgccaggc cgatagacac tgccgccgtc atctgggcag acatcccctg
    12541 agcgtaggtc atgcctttct taccgatttt ctcaccgata gtcgttgcca cacgacgcca
    12601 gccaatcatc gtaccgatac ctaacgccag cgcgaccgcc atgatgatcc acaccggtgc
    12661 atactcgatg gtgctaagca tgtcggactt cagtttcttc aacaggcgct gatcgtcagc
    12721 actcacgcca ggtatcttca ctactttgtc gatagtgtca gaaacgcaca gcataatgcg
    12781 gcgcatctgg ctacgttgat caagcgacag cttgtcgtag ctttccacat cggtagttaa
    12841 catacctttc aggcggttga gcgcgttaat ggtattcgac ggatggcaat ggaactccgc
    12901 gggttgcgtt gcgccagctt ccggagccgg tactaactga tcagcaccgg tagcctgttt
    12961 cagcagcgca ggatgctgct caaagtaagc ttcgacgttg ttgatggcat cacgggtacg
    13021 ggtgatttcg tagccagtgg cattcatatt caccacgaag cctgctggtg cgacaccaat
    13081 caataccaac ataaccagac caatgccttt ctgaccatcg ttcgcgccgt gcgaaaacgc
    13141 cacgccgata gcggaaagga tcagcgcaat acgcgtccag aacggcggct ttttcttgcc
    13201 gtctttcttt tcacgctccg ctggggtcag gtggatacgg gcgcgtttct tggtgccgct
    13261 ccagtagcga cgcagcaaga aaatcagacc gccagcaaac accaggccga caatagggga
    13321 aacaatcaga gaaccgaaaa tacttaatac tttcgggata ttgagtgcat ccaccactga
    13381 cgtcccggtc atcaacgcat tggttaaacc aatcccgatg atcgcgccaa tcagtgtatg
    13441 agagctggat gcaggtaaac caaagtacca ggtacccagg ttccagataa tcgccgccag
    13501 caacatagag aacaccatgg caaggccatg agacgatccc atattaagca gcagatccgt
    13561 cggcagcata tgcacaatgg cataggcaac actcagacca cccagcaaaa cacccaaaaa
    13621 gttgaacacc gccgccataa ccacggcgag ctgagaacgc atcgcgcggg tatagataac
    13681 ggttgccacg gcgttggctg tgtcatggaa accattgatg gcttcgtaga acagcacaaa
    13741 agccagtgca agcaataata acagcccggt atgcaaatcc aggccagcaa acaaatgtag
    13801 cataggacgt tacgccattt tgaggacatg aacgcggcgc attatcagtg actttcgcgg
    13861 cgcgggcaaa gtgaaatata gacttttttt gatttgcctc ctgtatggat ttcactcaaa
    13921 aaataattat cttatataat tcaggcaaat acttcctttt agtaatattg atgctggtgt
    13981 gaccactgag gaatctttac aattcacgcc cgttttttct aagaggagcg caacgtggaa
    14041 aggtttgatg ccattattat aggcgctggt gcggcgggta tgttctgttc tgcgctggca
    14101 ggtcaggcag gacgccgggt tctgctgatc gataatggta aaaaaccagg gcgcaaaatc
    14161 cttatgtctg gcggtgggcg ctgcaacttt accaaccttt atgtcgaacc aggcgcttat
    14221 ctgagccaga atccgcattt ttgtaagtct gcactcgcgc gttttaccca gtgggatttc
    14281 attgatctgg tcaataaaca cggcatcgcc tggcacgaga aaacgttagg acaactcttc
    14341 tgcgatgact ccgcgcagca gattgtcgac atgctggtgg atgagtgcga gaagggcaat
    14401 gtaaccttca gattgcgtag cgaagtgctg agtgtggcga aggatgaaac aggcttcacg
    14461 cttgaactga acggcatgac tgtcggttgc gaaaagctgg tcatcgcgac cggtgggctg
    14521 tcaatgccgg ggctgggcgc atcgccgttt ggttataaga ttgccgaaca atttggcctc
    14581 aacgtgctgc cgacccgtgc gggtctggtg ccattcactc tgcataaacc gttgctcgaa
    14641 gagttacagg tgctggcggg cgtggcggtg ccttccgtga ttaccgctga aaacggcacc
    14701 gttttccgtg agaacttact cttcacccac cgcggcttat ctggaccggc ggtgttgcag
    14761 atttccagct actggcaacc gggtgaattt gtcagtatca atctgctacc ggatgtggac
    14821 ctcgaaacct tcctgaatga gcagcgtaac gcacatccga atcagagcct gaaaaacaca
    14881 ctggcggttc atctaccgaa gcggttggtt gaacgcttac agcaactcgg gcaaatcccg
    14941 gatgtttcgc taaaacagct caacgtgcgt gaccaacagg cactgattag cacattgacc
    15001 gactggcgcg tacaacccaa cggcactgaa ggctatcgca ctgccgaagt gacgctcggc
    15061 ggcgtggaca ccaacgaact ctcttcacgg acgatggaag cgcgcaaagt gcctgggctg
    15121 tacttcatcg gcgaagtgat ggacgttacc ggctggctgg ggggctataa cttccagtgg
    15181 gcgtggtcga gtgcgtgggc ttgtgcgcag gatttgattg cagcgaagtc gtcctgacct
    15241 gttacctaaa gatgaaaatt attagagccg cattaaaaat gagctaattt tgatagtggc
    15301 tatcttgtga ttattttcta atgagcccgt gaactgaaac cctccaggct taatataagg
    15361 tggaaggaaa gatgattgaa aactcaccca gtggccagat ctttataaaa tatgaacagg
    15421 ctgaaaaaga cagtaacttt gttgtttttt atccttaacc actatgcatt aatgctgcgt
    15481 tatttcatga tgcctaagaa aaaccagcgt tacgcaaatg gtcaacgctg gttttatccg
    15541 gtacgttgca attatttttt agcagaacct gcttctaata ttgaaacgat tgagaacaac
    15601 gtaaagcata ccgctcccaa accgaccaaa acccgcgaag gaataaaata tcccatgtcg
    15661 gtctgcgcca tttccgcaag aaacgatgcc aggaaaaggc aaaacaggca ggtgaagacg
    15721 ggaatcatcg gtattctgtt ggctaacgaa caggtacgtc tccataccag tgccagtagc
    15781 cagacttttg agaatatgct gtaacagatc attccgaggt aaataagaat aatgccggga
    15841 gccagtcggg cgcttgcatc ggaactgact aagacgtata tcccctgcag taccgtgatt
    15901 gagccgagaa aaataaccca ataacaccac agccagtgtt ctttagttga aaacgtatta
    15961 cgtgtttgat ggacaattgt ggcaacaagg ccaattagac aggcacagat tgcggttaac
    16021 ccaagcaata cgtgacccgc gacataatgc ggagttatgt cagcactacg taacagcgta
    16081 atagaccaaa tgaaaccgag cagggtgagt aaaacgggaa cggcaatgag gcagttaccg
    16141 attaatgaag aataagcctg taccggtgtt ccgtcgctct tgctccccgc tgcatttttg
    16201 ggaatgagca gaaagtgacc ggacgacgct gccaccgtcg atacacatgc ggcaatcata
    16261 ccaatgccga aaataacata gccggcgaca aactcgtctg ccatcacatc attgcctgct
    16321 aacagtgccc aaccccatat catggtgata attgaccctg catagccaat aatggggaac
    16381 aatatattgt aaaacgtatt aacgccgcgc gtgagctgcg aaatgataat aaatgcggta
    16441 gtgaataatg ccaggcatat ggctgccaga gaaatcagca catgacccgc gacgaaatac
    16501 tcgctttgcc ctgaactttc cagcacaaag ccacctaacg cgatacaaat taagcccata
    16561 atcaaaggga ttaatttgaa aagccacccg atatatatgt tcactttaaa ctcctcaggt
    16621 at
//
LOCUS       DAJVUJ010000090        16609 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.090, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000090 DAJVUJ010000000
VERSION     DAJVUJ010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16609
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.090"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(28..2217)
                     /locus_tag="OQQ48_002592"
     CDS             complement(28..2217)
                     /locus_tag="OQQ48_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
                     /protein_id="HCS1953316.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLGKEKRAFTLS
                     WLAALSVLLLLLCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVETEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSICRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(2426..2501)
                     /locus_tag="OQQ48_002593"
     tRNA            complement(2426..2501)
                     /locus_tag="OQQ48_002593"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2466..2468),aa:Ala,seq:ggc)
     gene            complement(2541..2616)
                     /locus_tag="OQQ48_002594"
     tRNA            complement(2541..2616)
                     /locus_tag="OQQ48_002594"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2581..2583),aa:Ala,seq:ggc)
     gene            2837..3196
                     /locus_tag="OQQ48_002595"
     CDS             2837..3196
                     /locus_tag="OQQ48_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCS1953317.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            3198..3590
                     /locus_tag="OQQ48_002596"
     CDS             3198..3590
                     /locus_tag="OQQ48_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCS1953318.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGVRAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(3641..5056)
                     /gene="gltX"
                     /locus_tag="OQQ48_002597"
     CDS             complement(3641..5056)
                     /gene="gltX"
                     /locus_tag="OQQ48_002597"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCS1953319.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGR
                     TRSIERINKALAFIAERENQQ"
     gene            5316..5391
                     /locus_tag="OQQ48_002598"
     tRNA            5316..5391
                     /locus_tag="OQQ48_002598"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5349..5351,aa:Val,seq:tac)
     gene            5436..5511
                     /locus_tag="OQQ48_002599"
     tRNA            5436..5511
                     /locus_tag="OQQ48_002599"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5469..5471,aa:Val,seq:tac)
     gene            5558..5633
                     /locus_tag="OQQ48_002600"
     tRNA            5558..5633
                     /locus_tag="OQQ48_002600"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5591..5593,aa:Val,seq:tac)
     gene            5638..5713
                     /locus_tag="OQQ48_002601"
     tRNA            5638..5713
                     /locus_tag="OQQ48_002601"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5671..5673,aa:Lys,seq:ttt)
     gene            5833..6174
                     /locus_tag="OQQ48_002602"
     CDS             5833..6174
                     /locus_tag="OQQ48_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HCS1953320.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLDVSLLNP
                     VEKTTHIDIYI"
     gene            complement(6165..7091)
                     /locus_tag="OQQ48_002603"
     CDS             complement(6165..7091)
                     /locus_tag="OQQ48_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1953321.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALLLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            7181..8179
                     /locus_tag="OQQ48_002604"
     CDS             7181..8179
                     /locus_tag="OQQ48_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HCS1953322.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCTLPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(8176..8394)
                     /locus_tag="OQQ48_002605"
     CDS             complement(8176..8394)
                     /locus_tag="OQQ48_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HCS1953323.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(8396..10411)
                     /gene="ligA"
                     /locus_tag="OQQ48_002606"
     CDS             complement(8396..10411)
                     /gene="ligA"
                     /locus_tag="OQQ48_002606"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCS1953324.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(10482..11468)
                     /gene="zipA"
                     /locus_tag="OQQ48_002607"
     CDS             complement(10482..11468)
                     /gene="zipA"
                     /locus_tag="OQQ48_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HCS1953325.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPVYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            11698..12459
                     /gene="cysZ"
                     /locus_tag="OQQ48_002608"
     CDS             11698..12459
                     /gene="cysZ"
                     /locus_tag="OQQ48_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HCS1953326.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            12644..13615
                     /gene="cysK"
                     /locus_tag="OQQ48_002609"
     CDS             12644..13615
                     /gene="cysK"
                     /locus_tag="OQQ48_002609"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCS1953327.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            13999..14256
                     /gene="ptsH"
                     /locus_tag="OQQ48_002610"
     CDS             13999..14256
                     /gene="ptsH"
                     /locus_tag="OQQ48_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HCS1953328.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            14301..16028
                     /gene="ptsI"
                     /locus_tag="OQQ48_002611"
     CDS             14301..16028
                     /gene="ptsI"
                     /locus_tag="OQQ48_002611"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HCS1953329.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            16069..16578
                     /gene="crr"
                     /locus_tag="OQQ48_002612"
     CDS             16069..16578
                     /gene="crr"
                     /locus_tag="OQQ48_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCS1953330.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLPGSVTVG
                     ETPVIRIKK"
BASE COUNT         4197 a         4274 c         4074 g         4064 t
ORIGIN      
        1 gataattccc cggttttttt acgcctgtta atcagctaat ggctgcgggc gaccaatcaa
       61 atacccttgc agatattgca ccccgagctt atgcaatagc gcctgctgct gttgcgtctc
      121 gacaaactcc gcgaccacac tcaatgactt cgctttcgcc agatcggtaa ttgatctcac
      181 aatcatcgca tccagcgtgt tcgtgacaat atctttcaca aagacgccat caattttgat
      241 gatatcagcc tgcaaacgct ttaaccgttc gtagttggca tagccggtgc caaagtcatc
      301 aatcgcaatc cggaaaccaa acttatgcag ctgctcgatg ttgtacatgc tgctttctgc
      361 gttagaaaac gcctgctcct cggtgatctc aagaatgacc gcctgcgggg agatgtgata
      421 acgcttaaac agacgaataa tccgcccggc aatattcttt tgcagcagcg tgagcggcat
      481 taaattgact gaaaagcgcg gtcctttttt gtcgcaaggg tgtgttgcca gccacttcaa
      541 caaggattcc agcacttgca aatcaaaacg cgcgctaagg ttaaactgag caataagggg
      601 cagaaactta tccggggtca taatgccgcc gtcatatttc agtcgcgcga ggatctcatc
      661 ataaccttca ccctctttgt tgcgaattgg ctgggcgtag agcagcaaat caccctgatc
      721 taacgccgtg cgaatggtat tcagcaatag cacctgttta gtggtctgcc cggaaaccat
      781 ctcctctctg ctatccagcg ccagcacatg atgatgtgcg caggattgct ccgccagcca
      841 gcttaactgc cccaacaagg gttgcagggt ttcctgattt ccatcaaaac gcccccaggc
      901 agcaccatag cccatatcca gcccggtatt gttccagtga atttgccgac tattcaggat
      961 gttaaccata tgctggagtc gcccttccgt ttccggcccg ctcagcacta acagcagttc
     1021 actacccggc aattgataca acttttcgtt ttcctgcatc aacggcagca gcgtacggca
     1081 aattgagcgg atacagtgaa cgcgcatcat taagccgtaa tgacgactca taaactcaag
     1141 attatcaatg cgcaggcagc aaaaactctt gcccgcctct tgctccggcg cttgttccaa
     1201 cgcacggaag ttgggcagga gcgttaacgg atcggtcagc gcctgcaaat gccagcggcg
     1261 attaagccat tcactgcgat gataaatgcg caccatatag agcaggcaaa cgctaaagga
     1321 aatcagcacc gcaagaataa atgccagcga atattcggtt tccacccctt gcaaaaagtt
     1381 ctggttgtaa ttcagaaggc aaagcgttga aacagcccag gtgagattta aaaacggata
     1441 gcgaagctta ccgaccccga gggtaaagat gatgaagaaa acgggtacca ggtaaccggc
     1501 aataaagtcg ttttcataag gtgtgcacaa cagaagtaga agcacgctta acgctgccag
     1561 ccagcttaag gtaaatgcgc gtttctcttt gcccaacgac ggcgcgatat ccctgcgcca
     1621 caatatctgc gcaaagtggg gacttacaat catgcgagtg agatagtaga aaagcatgtt
     1681 gtaaatcagc acagcggtga aaaggcttag caaatcaacg accgtgaaaa tggcatccgc
     1741 atcgccgaaa aaggtggata tctttagcgg gaaatcaaag aagctcccca caagatacat
     1801 gctgcatttg atgccgatcg gcgtcaccaa accaagccag aacagacgtt gccagacata
     1861 tcggctggtc aatccataac gccaacgcgt ccccagctgc cagcgtaata ttgcgcaggc
     1921 acacaacacg acaaacgtct ggctaccgag catgaccgca gtctgtaaaa aagagagatg
     1981 aaagttccac agattggtgc aaaacatccc gcataaaacg ggaacgacac ctcgccagcc
     2041 aaagataaac aagattgaca gcattacgca cagtggcatc cacgccagaa agatataact
     2101 ggaatggata atggcaagtg gcgaaataaa acgggatagc tgaataagta ccacggtgag
     2161 cgtaaacgct agtgtgaata tcttgatatt ttttatcagg ttatgctcca caaacatgag
     2221 tacactactc tcactgatcg gagttttcag gcacacaaat aataagttta ttcgcatcgc
     2281 gaagcaaaca gggggccagc ctgttgccga tttttcatcc aatgatgctt tcaccaggcg
     2341 gcactaaccc ggacgtagac tgtattacaa aagcggcaaa aagcagagac aaaaaacccc
     2401 cgctttgcag cgagggttgg aaatttggtg gagctaagcg ggatcgaacc gctgacctct
     2461 tgcatgccat gcaagcgctc tcccagctga gctatagccc cacgatgcgt ttacgtacca
     2521 aatttgctgg gtgcaaaatt tggtggagct aagcgggatc gaaccgctga cctcttgcat
     2581 gccatgcaag cgctctccca gctgagctat agccccgtca cgtaaagctt gtcgagttga
     2641 cgggcggcat catatgaatt ccgcccgaat gtgtcaacgg caaattgcaa cgtgtagttt
     2701 caatcgctga aaaatcaggc aaatgaacaa ttttggaaaa ccgctcgcat tcagtagtta
     2761 ttcatgtcac ggtttcctgt aaagtggtgt tataaaatga actactaata gacccacata
     2821 cattcaggga attgttatgt tcaaggagag gatgacacca gatgaacttg ccagactgac
     2881 cggctatagc cgccagacca ttaataaatg ggtacgcaag gaaggctgga caacgtcacc
     2941 aaaacctggc gtccagggtg gcaaagcccg actggttcac gtcaatgaac aggttcgtga
     3001 atatattcgc aatgctgaac gtccagaagg tcagggagaa gcgcctgccc tttctggtga
     3061 tgcgccgctt gaagtattgc tggtgacact ggcgaaagag atgacgccag ttgaacaaaa
     3121 acagtttaca tccttgcttc tgcgggaagg gattatcgga ttgttacaac gcttagggat
     3181 tcgcgatagc aaataatatg aaaagattac gcaataaaat gaccaccgaa gaactggctg
     3241 aatgcctcgg tgtggctaaa caaactgtta accgttggat cagagaaaaa ggctggaaaa
     3301 cggaaaaatt tcccggcgtg aaaggcggtc gtgccagact tattctggtc gatacgcaag
     3361 tttgcgagtt tattcagaac acgccagcct tccataacac accaatgttg atggaagctg
     3421 aagagagaat tgctgaatac gctcctggag tacgcgcccc ggcgtatcga caaatcatca
     3481 acgcgataga taatatgaca gacattgagc aggaaaaagt cgcgcaattt ttgtcacgtg
     3541 aaggaattcg caatttcctc gcccgtctcg atattgacga atcagcataa ataaaaaacg
     3601 gcaggatatt atctcctgcc gtttatcttt tatgcgctaa ttactgctgg ttttcgcgtt
     3661 cagcaataaa agccagcgct ttgttgatac gctcaatact gcgggtccta ccaattgcgt
     3721 gaacggtaac atccagtgcc ggagactgcc ccgcaccggt tacggcgaca cgcagcggca
     3781 taccaacttt acccataccc acttccagct catccgccgt cgcctgaata gcgtgatgaa
     3841 cgttttcagc ggtccagtca gtaatcgcgg ccagtttgtc acgaaccact tccagcggct
     3901 gacgcgctac cggacgcaga tgttttttcg cggcgtcggc atcgaactca gcaaaatctt
     3961 cgtagaaata acggcagctc tgtgccatct ctttcagcgt cttgcagcgt tcgcccagca
     4021 gtttcaccag atcagccagc tgcgggccgt tacgggtatc gatattttcc tgctcaatgt
     4081 gccactgtaa gtgagtagca acatactccg gcggcagcgc gttaatgtag tgatggttca
     4141 gccacagcag cttgtcggtg ttgaacgcac tggcagattt gctgacggca ttcagggtga
     4201 agtatttgat catctcttca cgagtgaaga tttcctgatc gccgtgggac cagcccagac
     4261 gcaccagata gttcagcagt gcttctggca aataaccgtc atcacgatac tgcattacgc
     4321 tgaccgcccc gtgacgtttg gacagttttt taccgtcatc gccgttgatc atagaaacgt
     4381 gcgcgtaaac cggcaccggc gctttcaggg ccttaagaat gttgatctgg cgtggcgtgt
     4441 tgttgatatg gtcttcgcca cggataacgt gggtgatttc catatcccag tcatcgacaa
     4501 ccacacagaa gttataggtt ggggaaccat cggtacggcg aataataaga tcgtccagtt
     4561 cctggttgct gaactcgatc ggaccacgga tctgatcgtc aaaaacaaca gaaccttcct
     4621 gcgggttagc aaaacgtaca acgcacggtt catcatcagc gtgatgctca tggctgtggc
     4681 ggcagcgacc gtcataacgc ggcttctcac ctttcgccat ttgctcttcg cgcagcgctt
     4741 ccaggcgctc tttagagcaa tagcatttat aagcagtgcc ctcttccagc atctgatcga
     4801 tcaccgcgtt gtagcgatca aaacgtttgg tctggtagta cggaccttca tcccactcca
     4861 ggctcagcca gttcatgcca tccataatgg cttcgatagc ttccggcgtg gaacgctcaa
     4921 gatcggtgtc ttcaatacgc agcacgaact caccgccgtg gttacgtgca aaaagccagg
     4981 agtaaagggc agtacgcgcg ccgccaacgt gcagatagcc tgttgggctt ggcgcgaagc
     5041 gagttttgat tttcatgaaa tggccttacg tttagaaaga tgccgacaac cggcaaatcc
     5101 tggaaaaatt gagtgggcga tattctatca ctcccgcctg attcctcaat gtagttcggg
     5161 tttctacctt acgcattatt gttttcgttg acaaattgcg caacacggtc tattttgcga
     5221 tcatcttgct taaatttacg acgaacgaac aatttcttta gaaaatgcgt tgactcattt
     5281 tgaactctcc ctataatgcg actccacaca gcggggggtg attagctcag ctgggagagc
     5341 acctccctta caaggagggg gtcggcggtt cgatcccgtc atcacccacc aactacttta
     5401 tgtagtctcc gccgtgtagc aagaaattga gaagtgggtg attagctcag ctgggagagc
     5461 acctccctta caaggagggg gtcggcggtt cgatcccgtc atcacccacc actttctcac
     5521 cagctaaatt tcttgtaaca atgtgaagta ccgaagtggg tgattagctc agctgggaga
     5581 gcacctccct tacaaggagg gggtcggcgg ttcgatcccg tcatcaccca ccacttcggg
     5641 tcgttagctc agttggtaga gcagttgact tttaatcaat tggtcgcagg ttcgaatcct
     5701 gcacgaccca ccaatgtaaa aaagcgccct aaaggcgctt ttttgctatc tgcgatttgt
     5761 aagattgatt tttcttctca agttccttgt tcttctaccg atattacctc gtacagtgac
     5821 gaggactgaa caatggccac tatctcttca acttcaattc cttccataca aacacagagt
     5881 agtaacaggg catcacaagg tagcgatgtt gcttcacaaa tcgcgcggat aagtcagcaa
     5941 atcatcaaac tcactcaaca aattaaagag attgtagaca ctagcggcag tgctgaagac
     6001 aagcaaaaac aggccgaatt gattcaacag caaatcaccc tactggaaac acaactggcg
     6061 cagttgcaga aacagcaggc agaaaaggcg caggagaaag agcagcgttt gtctctggat
     6121 gtatcccttc taaatcctgt tgaaaaaacg actcatatcg atatctatat ctgatacaac
     6181 ggatcccctt cccgaccagc cattagcgca ttgccctggt ctcgtaccac gtcccataac
     6241 gcttcagcgg cggtggaaag tgaacggttc ttcctgcgca ccagcatcaa ctggcgttca
     6301 acaaccggcg taatgcgctt taccaccagt gggctacctt caggcaatag tagcgccagt
     6361 gcgggaagaa tactgatgcc gatgcctgcg gctaccatcg ggaacagcgt cgccggatgt
     6421 ccaatctcct gcacaatatt cgcctgaata ccattgcgcg ccagcgctgc gtcaatcagc
     6481 ggtcggctgc ctgacgcgta atcctgcaac accaatttcg ctccctgtaa tgcctgccag
     6541 ggtacataat cttccacggc caaagcgcta tcgcgatggc aaagcagaaa gaaaggctcg
     6601 gaaagaatcg cttcacattg cagatcgccc actgggcctg gatcgatgac gatgccaaag
     6661 tcgacatctc cctggcgaat actttccatc acccactgct gcggacgatc gtgcaggaca
     6721 aactgaatat ctggatagcg gcgatggctt tcagcaatgc attgcggaat aagatgcgcg
     6781 gaaatggtct ggctggcagc gacccgaact tttccgctca gttgttgccc catacgcccg
     6841 gtatcgcgca acgtgctgtt aagttcatcc agcagtcgct caagacgcaa agccaactgc
     6901 tgccctgcat ctgtaagcac cacttcacgc gtggttctgt ccagcaggcg aacaccagta
     6961 tgattttcca gctccttcac actgtgactt actgccgact ggctcaagcc gatacgctct
     7021 cctgcacgac taaaactttt ctcctgcgct actgtgacga aaacctttaa ttgttttaaa
     7081 gaataattca tctgttttct tcatgaatgg atgcaataaa tcaattttat ttctcaaact
     7141 ggaagaagca caatagaacc atcgatcatc tggagtcttt atgaaacttt ttcgtatcct
     7201 cgatcctttc accttaaccc tgatcacggt ggtgttgctg gcctctttct ttccggccag
     7261 aggcgatttc gtccccttct ttgaaaatct gaccaccgca gctattgccc tgctgttctt
     7321 tatgcacggc gcgaagttgt cgcgtgaggc gattattgct ggcggtggtc actggcgact
     7381 gcatttgtgg gtaatgtgca gcaccttcgt gctgtttccg attctgggtg tactgtttgc
     7441 ctggtggaaa ccggtaaatg tcgacccgat gctctactcc ggttttctct acttgtgcac
     7501 tctcccggct accgtgcagt ctgcaatcgc cttcacgtca atggcgggcg gtaacgtcgc
     7561 ggcggcggtt tgttctgcgt cagcatccag cctgctggga attttccttt caccattgct
     7621 ggttggtctg gtgatgaatg ttcacggtgc agggggcagc cttgagcagg tcggtaaaat
     7681 tatgctgcaa ctgctgctgc cgtttgtgtt ggggcatctt tcccggccgt ggattggtga
     7741 ctgggtgtcg cgcaataaaa aatggattgc gaaaactgac cagacgtcca ttctgttggt
     7801 ggtttataca gcgttcagcg aagccgtcgt taatggtatc tggcataaag ttggctgggg
     7861 atcattgctg tttatcgtgg tggtcagctg cgttcttctg gctatcgtga ttgtagttaa
     7921 cgtctttatg gcacgccgac tgggcttcaa taaggcagat gaaattacta tcgtcttttg
     7981 tggttcgaaa aagagtctgg caaatggcat cccgatggca aacattctgt tccccacatc
     8041 ggtgatcggt atgatggtgc tgcccctgat gattttccat cagatccaat tgatggtctg
     8101 tgcggtgctg gcgcgtcgat acaaacgcca gaccgaacag ttacaggcgc agcaggaaag
     8161 cagcgccgat aaagcttaaa gcggacgctt caggggctgg accagttgcg tcagcccctc
     8221 ggttttaatc agcagcgtga tttgcattag ctcaccgagc ttccctgccg ggaactcatc
     8281 tttgcgggca aaccacagca gatactcctc cggcaggtcg attaagcgac gccctttgta
     8341 tttgccaaac ggcattatcg tattggctat ttcaatcagc tgctcttttt ccatctcagc
     8401 tacccagcaa acgcagcatt tccgcttcgt cgatgacttc aatgcccagt tcctgcgcct
     8461 tcgccagttt agatcctgca gcttcacccg caatcaccag atcggttttc ttcgacacgc
     8521 tgcccgcgac tttcgcgccc agttcgacca gtcgagcttt agcgtcatca cgcgacatct
     8581 ggcttaagct gcccgtaagc accacggttt taccagcaaa cgggctgtca atctcttccg
     8641 cgttgataac gatcggcgca ggccagtgaa caccttccgc caacagctcg ctgatgacat
     8701 tgcggttgct ttcttcggca aagaagttgt gaacgtggga tgcaacgaca atgccaacat
     8761 caggcacctt ttgcagctct tcaatcgaag cggcttccag cgcttccagc gtgccgaaat
     8821 atgccgccag acctgctgcg gtggcctcgc cgacttcacg gatgccaagt gcatagagga
     8881 agcgagcaaa ggtggtttct ttcgcttttt ccagcgcgtt aaccacgttt tgtgccgatt
     8941 ttggccccat acgctccagt ccggtcagtt tgcctgcggt gagtttgaac agatctgccg
     9001 gagtgtggac atattctttt tcaaccagct gatcgatgat tttgtcgccc attccgtcaa
     9061 catccatcgc acggcgggaa acaaagtgtt tcagcgactc tttacgctgc gcaccgcaaa
     9121 tcaggccacc ggtacagcgg gcaaccgctt caccttccac acgctcaacg tcagaaccac
     9181 ataccggaca atgcgtcggg aatacaacct cacgggtatc ttccgggcgt tcagaaagca
     9241 cgacgttaac cacctgcggg atcacgtcgc cagcgcggcg aatcaccact ttatcgccaa
     9301 tgcgtaaacc aagacgttcg atttcatccg cattgtgtaa ggttgcgtta ctcaccagca
     9361 cgccagcaac atggacaggt tccagacgcg caacaggcgt aatcgcgcca gtacgcccaa
     9421 cctgaaactc gacgtcacgc acaaaggtca tctgctcctg cgccgggaat ttaaacgcta
     9481 ccgcccagcg cggggcacgc gcgacaaagc caagctgctc ctgctgttcc agtgagttga
     9541 ccttaatcac cacgccgtcg atatcaaagc ccagcgtcgg gcggtcttct tccactttgt
     9601 gatagaacgc cagcacttct tccgccgatt cacaaagcgt tacccgatcg ctgaccggca
     9661 acccccactt tttaaattgc agtaaacggc caagatgagt atccggcagc tcgccacctt
     9721 ccagaacacc aacgccatag cagaaaaaag tgagcggtcg cttcgctgta atacgcggat
     9781 caagctgacg cagtgaacca gctgccgcat tacgtgggtt agcaaaaact ttcccgcccg
     9841 tgcgtcgcgc atcttcgtta atcttttcga accccgcctg cggcaggaac acttcaccac
     9901 gcacttccag acgcgccggg atattctctc catgcagctt cagcggaatg gcgcgaatag
     9961 tacgcacatt agacgtgata tcttccccgg tggtgccatc gccacgggtc gcggcactga
    10021 ctaaaacgcc attttcatac agaatactga cggcaaggcc atccagcttc agctcacagc
    10081 accaggtgac tttctcgttg tttttcagac ggtcctgcac acgtttgttg aaagcaagaa
    10141 agctttcttc atcaaaaacg ttatccagtg acagcattgg tacttcatgg cgtatctggc
    10201 tgaaagccgc cagcggcgca gcacctacac gttgggtagg cgaatcaggc gtaatcagtt
    10261 ctggatgttt agtttccagc tcgcgcagtt cgcgcatcag cctgtcgtat tcagcgtcgg
    10321 gaatttccgg cgcatccatc acatgataaa gatattcatg atggcgaagc gtcgttcgca
    10381 gttctgtcag ttgttgttcg attgattcca tatcgcacca tcaatgctaa aaacccccga
    10441 caagcggggg ttcgaagaag agttaatttg ccttaagtgt atcaggcgtt ggcgtctttg
    10501 acttcgcgga tgatgtcctg gtactcgcgc aatttctgcg gagtcatcat acggcgctga
    10561 tcgtcaagca cgacaccgcc cacttcatcg gcaatatgct gcgcagattg cagcatcagc
    10621 ttgaagttct gcagctcgtc accgtaagac ggtacctgca taaagatagt gacacccgga
    10681 gtagtgaaat ccttcatttc aggatcaaag gttcccggtt tcaccatatt cgccaggctg
    10741 aataacgccg ggccgctgcc atccgggcta agatgacgat ggtaaatatt catatcgcca
    10801 aaaatgaagc ccgcttgttg aatgctgtta agaagcagtt caccgtttag ctcgctaccg
    10861 tgatgcgccg cgacgttcat gataatcacc gcttctttgc gcttcggttt atccataact
    10921 ggagccggtt ccgctacagg ctcaggctgt ggtgccgcta cgggttctgc aggctggaaa
    10981 gcctgttgtg ccggttgcgg tgctgaatgc acaggctgcg gcgctggcgc gacctgtggc
    11041 gaaactggct gctgcaacgg ctgttcaggc tgcggctgat agacaggctg ctgcaccggc
    11101 tgcgctggac gcggagcatg ttgcggcggt acctgcgctt caggcggctg ctggaccggt
    11161 tgacgcggct gcgcagacgc ataaggcggt tggtactggt gttgcggcga cggacgagca
    11221 gcctcatgct cctgagcgtt agccggggca tgattcacgc ggtgaacacg aacctcacca
    11281 acgccctcat catcttcgac atcctcgtca taagaatcgt cgtcacgttt tgacttcatt
    11341 cgttttaatg gccgatcgcg gaacatagaa gatcgttctt tacggctggt ccagaaacca
    11401 tgtaccagta aagcgattat ggcgatcgcg ccaacaatga ttaatatcag acgcaaatcc
    11461 tgcatcatta tattctctgt tgttctaaca ccttgccaca acggcaaaca tttactcact
    11521 aagagtattt gccgattacc tcaagtgcaa gtgcactatt aactttcaca gcacaaagat
    11581 agatgaaatc gtgctttttg ctgttttttc gaacatatcc taactgtcca ttgcgcaatt
    11641 acccggtaaa atacgcagaa ttttcctagg attggtcaaa aggagctcat cctgattatg
    11701 gtttcatcat tcacatctgc cccacgcagc ggtttttact attttgcgca aggctggaag
    11761 ctcgtctcgc aacctgggat tcggcgtttc gttattttac cgctgctggt caatattttg
    11821 ttgatggggg gcgcattctg gtggctcttt acacagctcg atgtctggat cccgactctc
    11881 atgagttacg ttccggactg gctacaatgg ctgagttatc tgttgtggcc tctggctgtc
    11941 atctctgtgc tgttagtgtt tggctatttc ttctccacga ttgctaactg gattgccgct
    12001 ccgtttaacg gtttattggc tgaacaactg gaagcacgat tgactggcgc tacaccgcca
    12061 gataccggga ttttcggtat catgaaagat gtgccgcgaa tcatgaaacg cgaatggcaa
    12121 aaatttgcct ggtatctgcc gcgcgcaatt gtattactaa ttctttactt catccctggt
    12181 attgggcaaa ccgtcgcgcc ggtactgtgg ttcctgttta gcgcctggat gttagccatc
    12241 cagtattgcg attacccctt cgataaccac aaagtgccgt ttaaagagat gcgcaccgcc
    12301 ctgcgcacac gcaaaatcac caatatgcag tttggtgctt taaccagcct gtttacgatg
    12361 atcccgctgc ttaatctgtt catcatgccc gttgccgttt gtggcgcgac ggcgatgtgg
    12421 gtcgattgct atcgcgataa acacgcgatg tggcggtaac aatctaccgg ttattttgta
    12481 aaccgtttgt gtgaaacagg ggtggcttat gccgcccctt attccatctt gcatgtcatt
    12541 atttcccttc tgtatataga tatgctaaat ccttacttcc gcatattctc tgagcgggta
    12601 tgctacctgt tgtatcccaa tttcatacag ttaaggacag gccatgagta agatttttga
    12661 agataactcg ctgactatcg gtcacacgcc gctggttcgc ctgaatcgca tcggtaacgg
    12721 acgcattctg gcgaaggtgg aatctcgtaa ccccagcttc agcgttaagt gccgtatcgg
    12781 tgccaacatg atttgggatg ccgaaaagcg cggcgtgctg aaaccaggcg ttgaactggt
    12841 tgaaccgacc agcggtaata ccgggattgc actggcctat gtagctgccg ctcgcggtta
    12901 caaactcacc ctgaccatgc cagaaaccat gagtattgaa cgccgcaagc tgctgaaagc
    12961 gttaggtgca aacctggtgc tgacggaagg tgctaaaggc atgaaaggcg caatccaaaa
    13021 agcagaagaa attgtcgcca gcaatccaga gaaatacctg ctgctgcaac aattcagcaa
    13081 tccggcaaac cctgaaattc acgaaaaaac caccggtccg gagatctggg aagataccga
    13141 cggtcaggtt gatgtattta ttgctggcgt tgggactggc ggtacgctga ctggcgtcag
    13201 ccgctacatt aaaggcacca aaggcaagac cgatcttatc tctgtcgccg ttgagccaac
    13261 cgattctcca gttatcgccc aggcgctggc aggtgaagag attaaacctg gcccgcataa
    13321 aattcagggt attggcgctg gttttatccc ggctaacctc gatctcaagc tggtcgataa
    13381 agtcattggc atcaccaatg aagaagccat ttctaccgcc cgtcgtctaa tggaagaaga
    13441 aggtattctt gcaggtatct cttctggagc agctgttgcc gcggcgttga aactacaaga
    13501 agatgaaagc tttaccaaca agaatattgt ggttattcta ccatcatcgg gtgagcgtta
    13561 tttaagcacc gcattgtttg ccgatctctt cactgagaaa gaattgcaac agtaatgcca
    13621 gcttgttaaa aatgcgtaaa aaagcacctt tttaggtgct tttttgtggc ctgcttcaaa
    13681 ctttcgcccc tcctggcatt gattcagcct gtcggaactg gtatttaacc agactaatta
    13741 ttttgatgcg cgaaattaat cgttacagga aaagccaaag ctgaatcgat tttatgattt
    13801 ggttcaattc ttcctttagc ggcataatgt ttaatgacgt acgaaacgtc agcggtcaac
    13861 acccgccagc aatggactgt attgcgctct tcgtgcgtcg cgtctattaa aaactggcgc
    13921 taacaataca ggctaaagtc gaaccgccag gctagacttt agttccacaa cactaaacct
    13981 ataagttggg gaaatacaat gttccagcaa gaagttacca ttacagctcc gaacggtctg
    14041 cacacccgcc ctgctgccca gtttgtaaaa gaagctaagg gcttcacttc tgaaattact
    14101 gtgacttcca acggcaaaag cgccagcgcg aaaagcctgt ttaaactgca gactctgggc
    14161 ctgactcaag gtaccgttgt gactatctcc gcagaaggcg aagacgagca aaaagcggtt
    14221 gaacatctgg ttaaactgat ggcggaactc gagtaatttc ccgggttctt ttaaaaatca
    14281 gtcacaagta aggtagggtt atgatttcag gcattttagc atccccgggt atcgctttcg
    14341 gtaaagctct gcttctgaaa gaagacgaaa ttgtcattga ccggaaaaaa atttctgccg
    14401 accaggttga tcaggaagtt gaacgttttc tgagcggtcg tgccaaggca tcagcccagc
    14461 tggaaacgat caaaacgaaa gctggtgaaa cgttcggtga agaaaaagaa gccatctttg
    14521 aagggcatat tatgctgctc gaagatgagg agctggagca ggaaatcata gccctgatta
    14581 aagataaaca catgacagct gacgcagctg ctcatgaagt tatcgaaggt caggcttctg
    14641 ccctggaaga gctggatgat gaatacctga aagaacgtgc ggctgacgta cgtgatatcg
    14701 gtaagcgcct gctgcgcaac atcctgggcc tgaagattat cgacctgagc gccattcagg
    14761 atgaagtcat tctggttgcc gctgacctga cgccgtccga aaccgcacag ctgaacctga
    14821 agaaggtgct gggtttcatc accgacgcgg gtggccgtac ttcccacacc tctatcatgg
    14881 cgcgttctct ggaactgcct gctatcgtgg gtaccggtag cgtcacctct caggtgaaaa
    14941 atgacgacta tctgattctg gatgccgtaa ataatcaggt ttacgtcaat ccaaccaacg
    15001 aagttattga taaaatgcgt gctgttcagg agcaagtggc ttctgaaaaa gcagagcttg
    15061 ctaaactgaa agatctgcca gctattacgc tggacggtca ccaggtagaa gtatgcgcta
    15121 acattggtac ggttcgtgac gttgaaggtg cagagcgtaa cggcgctgaa ggcgttggtc
    15181 tgtatcgtac tgagttccta ttcatggacc gcgacgcgct gcccactgaa gaagaacagt
    15241 ttgctgctta caaagcagtg gctgaagcgt gtggctcgca ggcggttatc gttcgtacca
    15301 tggacatcgg cggcgacaaa gagctgccat acatgaactt cccgaaagaa gagaacccgt
    15361 tcctcggctg gcgcgctatc cgtatcgcga tggatcgtaa agagatcctg cgcgatcaac
    15421 tccgcgctat cctgcgtgcc tcggctttcg gtaaattgcg cattatgttc ccgatgatca
    15481 tctctgttga agaagtgcgt gcactgcgca aagagatcga aatctacaaa caggaactgc
    15541 gcgacgaagg taaagcgttt gacgagtcaa ttgaaatcgg cgtaatggtg gaaacaccgg
    15601 ctgccgcaac aattgcacgt catttagcca aagaagttga tttctttagt atcggcacca
    15661 atgatttaac gcagtacact ctggcagttg accgtggtaa tgatatgatt tcacaccttt
    15721 accagccaat gtcaccgtcc gtgctgaact tgatcaagca agttattgat gcttctcatg
    15781 ctgaaggcaa atggactggc atgtgtggtg agcttgctgg cgatgaacgt gctacacttc
    15841 tgttgctggg gatgggtctg gacgaattct ctatgagcgc catttctatc ccgcgcatta
    15901 agaagattat ccgtaacacg aacttcgaag atgcgaaggt gttagcagag caggctcttg
    15961 ctcaaccgac aacggacgag ttaatgacgc tggttaacaa gttcattgaa gaaaaaacaa
    16021 tctgctaatc cacgagatgc ggcccaattt actgcttagg agaagatcat gggtttgttc
    16081 gataaactga aatctctggt ttccgacgac aagaaggata ccggaactat tgagatcatt
    16141 gctccgctct ctggcgagat cgtcaatatc gaagacgtgc cggatgtcgt ttttgcggaa
    16201 aaaatcgttg gtgatggtat tgctatcaaa ccaacgggta acaaaatggt cgcgcctgta
    16261 gacggtacca ttggtaaaat ctttgaaacc aatcacgcgt tctctatcga atctgatagc
    16321 ggcgttgaac tgttcgtcca cttcggtatc gacaccgttg aactgaaagg cgaaggcttc
    16381 aagcgtattg cagaagaagg tcagcgcgtg aaagttggcg atactgtcat tgaatttgat
    16441 ctgccgctgc tggaagagaa agccaagtct accctgactc cggttgttat ctccaacatg
    16501 gacgaaatca aagaactgat caaactgccc ggtagcgtaa ccgtgggtga aaccccggtt
    16561 atccgcatca agaagtaatt cttgccgcag tgaaaaatgg cgcccatcg
//
LOCUS       DAJVUJ010000091        16572 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.091, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000091 DAJVUJ010000000
VERSION     DAJVUJ010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16572
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.091"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..2017
                     /gene="fhuE"
                     /locus_tag="OQQ48_002613"
     CDS             <1..2017
                     /gene="fhuE"
                     /locus_tag="OQQ48_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HCS1953331.1"
                     /translation="DYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLEISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAIDMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSGWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNMEGSIVYGAPRNFSITGTYQF"
     gene            complement(2077..3510)
                     /gene="ptsG"
                     /locus_tag="OQQ48_002614"
     CDS             complement(2077..3510)
                     /gene="ptsG"
                     /locus_tag="OQQ48_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HCS1953332.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMHDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(3805..4602)
                     /locus_tag="OQQ48_002615"
     CDS             complement(3805..4602)
                     /locus_tag="OQQ48_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCS1953333.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(4613..5617)
                     /gene="holB"
                     /locus_tag="OQQ48_002616"
     CDS             complement(4613..5617)
                     /gene="holB"
                     /locus_tag="OQQ48_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCS1953334.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYTVTWLSREVTMSQDALLAALRLSAGSPSAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(5614..6255)
                     /locus_tag="OQQ48_002617"
     CDS             complement(5614..6255)
                     /locus_tag="OQQ48_002617"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCS1953335.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(6245..7267)
                     /gene="yceG"
                     /locus_tag="OQQ48_002618"
     CDS             complement(6245..7267)
                     /gene="yceG"
                     /locus_tag="OQQ48_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HCS1953336.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(7270..8079)
                     /gene="pabC"
                     /locus_tag="OQQ48_002619"
     CDS             complement(7270..8079)
                     /gene="pabC"
                     /locus_tag="OQQ48_002619"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HCS1953337.1"
                     /translation="MFLINGHKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMIFCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTVYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(8199..9440)
                     /gene="fabF"
                     /locus_tag="OQQ48_002620"
     CDS             complement(8199..9440)
                     /gene="fabF"
                     /locus_tag="OQQ48_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCS1953338.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKAIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNLDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(9528..9764)
                     /gene="acpP"
                     /locus_tag="OQQ48_002621"
     CDS             complement(9528..9764)
                     /gene="acpP"
                     /locus_tag="OQQ48_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCS1953339.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(9975..10709)
                     /gene="fabG"
                     /locus_tag="OQQ48_002622"
     CDS             complement(9975..10709)
                     /gene="fabG"
                     /locus_tag="OQQ48_002622"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HCS1953340.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(10722..11651)
                     /gene="fabD"
                     /locus_tag="OQQ48_002623"
     CDS             complement(10722..11651)
                     /gene="fabD"
                     /locus_tag="OQQ48_002623"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCS1953341.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(11667..12620)
                     /gene="fabH"
                     /locus_tag="OQQ48_002624"
     CDS             complement(11667..12620)
                     /gene="fabH"
                     /locus_tag="OQQ48_002624"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HCS1953342.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVANQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(12688..13758)
                     /gene="plsX"
                     /locus_tag="OQQ48_002625"
     CDS             complement(12688..13758)
                     /gene="plsX"
                     /locus_tag="OQQ48_002625"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCS1953343.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLRPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(13839..14012)
                     /gene="rpmF"
                     /locus_tag="OQQ48_002626"
     CDS             complement(13839..14012)
                     /gene="rpmF"
                     /locus_tag="OQQ48_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCS1953344.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(14064..14585)
                     /gene="yceD"
                     /locus_tag="OQQ48_002627"
     CDS             complement(14064..14585)
                     /gene="yceD"
                     /locus_tag="OQQ48_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HCS1953345.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            14784..15368
                     /gene="yceF"
                     /locus_tag="OQQ48_002628"
     CDS             14784..15368
                     /gene="yceF"
                     /locus_tag="OQQ48_002628"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HCS1953346.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLCEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(15480..16439)
                     /gene="rluC"
                     /locus_tag="OQQ48_002629"
     CDS             complement(15480..16439)
                     /gene="rluC"
                     /locus_tag="OQQ48_002629"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HCS1953347.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRSQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
BASE COUNT         4104 a         4507 c         4001 g         3960 t
ORIGIN      
        1 tgattacagc gtaacgtcta cctctgcggg taccaaaatg cagatgactc aacgtgatat
       61 tcctcagtcg gtcactattg ttagccagca gcggatggaa gatcagcagt tacaaacgct
      121 gggcgaagtg atggaaaaca cgctggagat cagcaaaagt caggcggatt ccgatcgtgc
      181 tctttattat tcccgcggat tccagatcga taactatatg gttgatggta tccccaccta
      241 ttttgaatcg cgctggaatc tgggcgatgc actttctgat atggcactgt ttgaacgcgt
      301 agaagtagtg cgtggcgcga caggactcat gaccgggacg ggtaatccat ctgcggcaat
      361 tgatatggtt cgaaaacacg cgaccagtcg tgaatttaaa ggcgatgtct cggcggaata
      421 cggtagctgg aacaaagaac ggtatgtggc ggatttacaa agcccactca ccgaagacgg
      481 taaaatccgc gcgcgaattg tcggcggcta ccagaataac gactcatggc tggaccgcta
      541 caacagtgaa aagaccttct tctccggcat tgtcgatgct gatttaggcg atcttacgac
      601 gctttcagcc ggttacgaat atcagcgcat tgatgttaat agccctacct ggggcggttt
      661 accgcgctgg aatactgatg gcagcagcaa cagttacgat cgtgcacgca gtaccgcacc
      721 tgactgggcg tacaacgata aagagatcaa caaggtcttt atgaccctga agcagcggtt
      781 tgctgatacc tggcaagcga cactgaatgc cacccactct gaagtcgaat ttgacagcaa
      841 aatgatgtat gtcgatgcct atgtaaacaa agcggatggt atgctggttg ggccatacag
      901 taattatgga cctggctttg attatgtcgg cggcaccggt tggaacagtg gcaaacgtaa
      961 agttgatgcg ctagatttgt tcgctgacgg tagttatgaa ttgtttggtc gtcagcacaa
     1021 tctaatgttt ggtggcagtt acagcaaaca aaacaatcgt tacttcagtt catgggccaa
     1081 catcttcccg gatgaaattg gcagtttcta caactttaat ggcaatttcc cacaaaccga
     1141 ctggtcacca cagagcctgg cacaggacga taccacacat atgaaatcgt tatatgctgc
     1201 cactcgagtc acccttgccg atccgctgca tctgatcctc ggcgcacgct ataccaactg
     1261 gcgggttgat acgctgactt acagcatgga gaaaaaccac accacgcctt acgctggtct
     1321 ggtgtttgac atcaatgaca actggtcgac ctacgccagc tatacctcta ttttccagcc
     1381 gcaaaatgat cgtgacagtt caggcaaata tctggctcca atcaccggta acaactacga
     1441 gctgggtctg aaatcgggct ggatgaatag ccgtctgacc accacgttag ccatcttccg
     1501 tattgagcag gataatgtcg ctcagtccac cggtacacct atccccggca gcaacggcga
     1561 aaccgcctat aaagcggtgg atgggacagt cagtaaaggg gtggaatttg aactcaacgg
     1621 cgcaattacc gacaactggc agctgacatt tggcgcaacg cgctatattg cagaggataa
     1681 cgaaggaaac gccgttaatc ctaatctgcc acgcaccacg gttaaaatgt tcaccagcta
     1741 tcggttgcct gtcatgccag agttgacagt cggcggtggt gtgaactggc aaaatcgcgt
     1801 gtataccgac accgtgacac cgtatggcac cttccgcgcc gagcaaggta gctacgcgct
     1861 ggtggatctc ttcacccgct accaggtgac gaaaaacttc tcgttacagg ggaacgtcaa
     1921 taacctgttc gacaaaacct acgataccaa catggaaggt tctatcgtct acggcgcacc
     1981 gcgtaatttc agcattaccg gcacgtatca attctgaata acacctgtaa aaaaggcagc
     2041 catctggctg ccttagtctc cccaacgtct tacggattag tggttacgga tgtactcatc
     2101 catctcggtt ttcaggttat cggatttagt accgaaaatc gcctgaacac cagaacccgc
     2161 aaccactacg cccgctgcgc ccagtttctt caggccagcc tgatccactt tagacacatc
     2221 agcaacgctg acgcgcagac gggtaataca tgcgtcgagg ttagtaatgt tttctttacc
     2281 accaaatgca gcaaccagag ccggtgccat ttcgctggta cctgttgctt ttgcatcttc
     2341 agtcgcgtct tcacgacccg gcgttttcag atccagtgct ttaatcagca cgcggaagat
     2401 ggtgtagtaa acaatcgcat aaccgatacc gacgatcggg aacagccaca gtttgctgct
     2461 gttaccagac agaacgataa agtcgatcag accgtgcgag aacgacgtac cgtcatgcat
     2521 ccccagaaga atacagattg ggaatgccag gcctgccaga atcgcgtgga tgatgtacag
     2581 gatcggcgca acgaacatga aggagaactc gatcggctcg gtgataccgg tcaggaacga
     2641 ggtcagcgcc gcggagatca taataccgcc cactttcgcg cggttttctg gtttagcaga
     2701 gtgccagata gcaattgcgg cagctggcag accgtacatt ttgaacagga agccaccaga
     2761 cagtttaccc gcagtcgggt cacccgccat ataacgcgga atgtcgccgt ggaaaacctg
     2821 acctgctgcg ttggtgtatt caccaatctg catctggaaa ggtacgttcc agatgtggtg
     2881 cagaccaaac ggtaccaggc aacgttcgat gaaaccgtaa atgccaaacg caactaccgg
     2941 gttctggtaa gcagcccact gagagaaggt ctggattgca gaaccaatcg gcggccaaat
     3001 gaaggacagc acaacgccag taaagatggc agccaggcca gaaatgatcg gcacaaagcg
     3061 tttaccggca aagaagccaa gatactcagg cagcttaata cggtagaaac ggttaaacat
     3121 gtacgctgcg atcgcaccgg agataatccc tccgagtacg ccagtatccg ccaggtgttt
     3181 agaggcgatt tcttcagcag gtaaatgcag taccagtggc gcaaccacgg ccatggtttt
     3241 aaccatgatg ccataggcaa caactgcggc cagcgcggat acgccatcgt tattggtaaa
     3301 gccgagggcg acaccgatcg caaaaatcag tggcatgttt gcaaagacgg aaccgcctgc
     3361 ttctgccata acatgcgata caacggcggg cagccagctg aaattcgcgg aaccgacgcc
     3421 cagcagaata cctgcgatag gtagtacgga taccggcagc atcagcgatt taccgacctt
     3481 ttgcaggtta gcaaatgcat tcttaaacat aattgagagt gctcctgagt atgggtgctt
     3541 tttttacgtt ctcacgcgtg gcaagggggg agagcctcgc cgtgtacagg gcatctaagc
     3601 gccctttatt tattacacag agtaaaataa ttcagtgcca atttgtttga cggctatcac
     3661 gtttcaactt cagatcattg gagaagttgc acattttgac aaaacatatg tcacaatatt
     3721 tcaaacgcgg ttattcaccg cccaattttc ggcggtgaac tttactcgtt ttagccatta
     3781 attacgagct ttaaaaaaac tcattcaacg gatggattga aggcgggaag cgtcgatgtg
     3841 aaacagacgg gcgaagttat cggtggttac ctgcgccagt tcttcaacgg caacaccttt
     3901 caacacagcc atgtattctg caacgtcacg aaccatcgca ggttgattct cttttcctcg
     3961 atgcggtacc ggcgcaaggt aaggtgagtc agtttccacc agtaatcgat ccagggggac
     4021 ataacgcgca gcatcgcgca gttgctccgc attacggaag gtcacaatgc cggaaaagga
     4081 gatgtaaaat ccgagatcca gtaatttacc cgccgtttct ctgtcctctg taaaacagtg
     4141 tagtacgcca ccgcaatccg tcactttttc ttcgcgcaga attgccagcg tatcggcacg
     4201 ggcgtcacgg gtatggacga taaccggctt gttcagttca cgaccaatct ggatatgatg
     4261 gatgaaggac tcttgctgac gtactttagt ttccggtgtg taataataat ccagcccggt
     4321 ttcacccagc gctacaacac cctcttctgc cgccagacgg cgtaaatctt ctacatcgta
     4381 gggatcattc tggttgagtg gatgcacgcc acaagaaaat acgacgttgt cacgttcgcc
     4441 taccagatcc cgcatatgta aataacccgg taatgttgtg gcgactgcca gacaaaattt
     4501 cacatcgcgt gcggcggctt tcgccagaac gtcatccacg tccttatgca aagattcata
     4561 atccagacca tcgagatggc agtgtgagtc gactaaaaac atgatgtctc tcttaaagat
     4621 gaggaaccgg tagcacaacg cccggttgca ggtaatgctc aatacgcagt aaaagatcgg
     4681 tgatgagaag ctcgcggttg atgcctgtga cagacattaa ctgttcacga atgtggcaaa
     4741 catcccccag tatagcctgc aggcgcgagg gagaaagatg gtttgccagt tcggcgacca
     4801 ggcccggcac atcaacattg gtcacctgtg cagcgccatg atggcgtttt agcgcatcca
     4861 tcagcaacgt tgccagccag tgtaaacgcg ccggagcttg ttcatgatta agggccgcca
     4921 gcagcgaata ccagtcgccc gatggtacgc tatatgccaa cgcctgacac aatgtttcac
     4981 gagcctgcca gttatctccc tgaaacaacg ccagtgccgc gctaggcgaa ccggcactta
     5041 agcgcaatgc ggcaagtaat gcatcctgtg acattgtcac ttcgcgtgaa agccaggtca
     5101 cggtgtactg ttccggcggc ggcgcaaggt aatgtaaccg acaacgacta cgtaatgttg
     5161 ccagtaaacg ttcaggctcg cgggtagcca ggaaaaacca ggtttctgct ggtggctctt
     5221 caagcgtttt cagcaatgcg ttagccgcgg cgtcggtcag taaggcagca tcggttaccc
     5281 agacaacttt cgcaccacct aggcgtgcgt gctcattcag cttttcggtg acctcacgta
     5341 ccgcatcaat gcccagcgta tttttccctt tttcgggagc cagggtgtag taatcgggat
     5401 gcgtgccagc ctgcatcaac tgacatccac gacagtgacc gcaacttttg tggccctgcg
     5461 gttgttggca gagtaaataa cggctcaggg cgtagattaa agcatcatcg cccatgcccg
     5521 gtaacgcctg aatgagtagc gcatggtgac ctcttccggc ctgatagctg gctaccagtt
     5581 tttcgaaatc aggtcgtaac catggatacc atctcatgcg tccaactcct tcacccagtg
     5641 ggtcacggta gtacggattg catccatcac ggcctccagc ggctgggtgg catcaatggt
     5701 atgaatactt ttatcttgtg cagccagttc cagatagcgg gcgcgggtgc gattaaagaa
     5761 atcgaaagac tcttgctcaa tacgatccag ctcgccgcgc gcacgcgcgc gttttaagcc
     5821 aacttccggg gtaacatcga gatagagcgt taagtcaggg cgaaaatccc cgagaacagc
     5881 atcacgcagt gttgccagca tatgttggtc aataccacgt cctccgccct gatacgcctg
     5941 agtggagaga tcgtggcgat cgccaatcac ccaggtgccg ttagccagag ctggtttgat
     6001 gaccgtttct accagttgaa cgcgcgcggc ataaaacatc agaacttcgg ctttatcggt
     6061 aatgacttca tcgcctaccg atttgatatc cagcaccagg cttcttaact tttcggcaag
     6121 ttgcgtaccg ccaggttccc gagtgaaaac catgtcgcgg atacccagtt gctcaagcgt
     6181 ctcaaccacc acattacgcg cggtagtttt gcctgcgcct tccagcccct caatgacgat
     6241 atacttactg cgcatttttt tccttaagca ctttcagata atcctgcaca gacttgttat
     6301 ggctggcaag attggtatta aacgtgtgac cacctttacc atcggccaca aaatagagat
     6361 acggcgtttt tgccggatgc gcagcagcct tcagcgaatc cgcccccggc gtagctatcg
     6421 cacctggagg caaaccggta atggtatagg tgttatacgc tgtcggcgtt tccaggtctg
     6481 cacgagaaag tttgccatta taacgctctc ccatcccgta aatcacggtt gggtcggtct
     6541 gcaggcgcat accaatgcgt aaacggttga taaataccga ggcaacctga tcgcgttcac
     6601 tggcaacggc ggtttctttt tcgataattg atgccatcgt caccagctgg tttttatctt
     6661 tataaggcag accgtccgca cgcccttccc aggcgctatc gaccgctttc accattttct
     6721 tgtgcgctcg cttgagtaac gcgacatcgg tggtattggc ggtatacatc caggtgtctg
     6781 gccagaacca accttcaatc cactccgggt tttccagttc aagtgcctgc gctacggtgg
     6841 cgtacttatc gtcactcagc gtgtgcttga tatacggagc ctcacgcaat tgcttgaggt
     6901 agtcgctcag acgcatccct tctaccagtc gcagcgggaa ctgtgcttct ttaccgcttt
     6961 ccagcaattt cagcatctcg cgcacggtca tctgcggtgt aaagcggtaa gtcccggctt
     7021 taaagtgaga aagatccggt tcgatacgca gcaaccattg aaaaacccgt gggcgattga
     7081 tgatcttatc ggcataaagc tgttcaccaa gcgccagacg tccggtccct ggcttcaggg
     7141 taaatatcgt ctcttctttg ataagcaatt tgctgtcggc aagatggcga accttccaga
     7201 cgcccacacc agcggcgata cccagtacca ccagcaataa caagataatc aataacactt
     7261 ttttcatgac taattcgggc gctcacaaag tggggctaaa tattcatata acgttgctga
     7321 cgaaaaggag acatcgccac aggcacgtac gggcatcact ggcattaacg cattacaaat
     7381 aaccatctca tctgcctgca acgcctcttc cagagaggct tgcacttcga caagctgata
     7441 agaggattgt gccagcaaac ggatacagaa ttgtcgcata atgccgttaa cacctgcctg
     7501 atccagtcgc ggcgtataaa ctacattgcc cttccgccag aacaaattag ccgcacagca
     7561 ttccgtaacc cacccttcgc tgtcaaggac cagcgcctca tcagcgtttg tctgctcaag
     7621 atgagagcgg atcaatactt gctcaagccg attaagatgt ttaatacctg caagatgggg
     7681 attgcgcccc agccgcaccg ggcttagcgc caacgtcatc ccttcgttac gcaaacggtc
     7741 gtaatgtgca ggataaaccg taacggagag aatccgcgtt gccggtccac tgttcaatgt
     7801 gctgtaccct cgcccgccac taccgcgact gatcacgact ttcagcacac cattttgctg
     7861 ttctgctgcc agcgttttca tctcttgttc aagctgaggc cagaagtcac agaaaatcat
     7921 caaccgctga caagcatcct gtagtcgctg gatatgcgcc gataacaaac tgactttacc
     7981 gtcgataact ctggcggtgg taaaacaacc atcaccaaac tgcgttgccc gatcgcttac
     8041 tgccagcgat tcctgcttat gaccgttaat taagaacata ccggctcctt atcagagaat
     8101 tgttagtgtg gcagcatgct cactacggaa caagtcggaa taaaagctaa gaaaaaaggc
     8161 ccgcaagcgg accttttata tgggtgggaa atgacaactt agatcttttt aaagatcaaa
     8221 gaaccattag tgccaccgaa gccgaaggag ttacacagcg tgtattccat tccgctgacc
     8281 tgacgtgctt catgcggtac gaaatccaga tcgcaacctt catccaggtt atccaggttg
     8341 atggttggcg gaacagcctg atcgcgcagc gccaggatgg agtagataga ctctactgcc
     8401 cccgccgcac ctaacaggtg accggtcata gatttcgtgg agcttaccaa cacacggctt
     8461 gcagcttcac cgaagatggc tttcaccgcc tgcgcttcag ctttatcgcc agccggcgta
     8521 gaagtaccgt gcgcgttaac gtagccaatc tgactcgctt caatgcctgc atcacgcaga
     8581 gcatttgcca ttgccagagc tgcgcctgcg ccattttctg gcggtgacgt catatgataa
     8641 gcatcgctgc tcataccaaa gccgacgagt tcagcgtaaa ttttcgcacc gcgttttttc
     8701 gcgtgttcgt actcttctag taccagcata ccggcaccat cgcccagtac gaagccatca
     8761 cgctctttat cccacgggcg gctcgccgct tgcgggttat cattgcgggt agataatgca
     8821 cgtgccgcgc caaaaccacc aacgcccagc ggcgtactgg ctttctctgc gccacctgca
     8881 accatcacgt cagcatcgcc atacgcgata atacgcgcag catgaccaat gttgtgcacg
     8941 ccggaagtac aggcggtcgc gatagagatg cttgggccac gcaggccata catgatagtc
     9001 agatgacctg ccaccatgtt cacaatcgtt gacggaacga agaatgggct gatcttacgt
     9061 ggaccaccgt tcatcagaga tgtgtggttt tcttcgatca gtccgaggcc gccaatcccg
     9121 gagccaattg cggcaccaat gcgggttgcg ttctcttccg ttatttcaag gccagaatcc
     9181 tgcatggcct gaacgccagc gacaattcca tattgaatga aggcatccat cttgcgctgt
     9241 tctttgcgcg agataatgtc ctcacagtta aaatccttta ctaagccagc aaatttcgtt
     9301 gcataggcgc tagtatcgaa atggtcgatt aggctgatgc cactctgacc ggcaagcaga
     9361 gctttccagg tagactctac ggtattgccg acaggagaca acatgcccag tccggtcaca
     9421 actacacgac gcttagacac gtttgtcctc cagggaggga aaaaatgatt ctagtgggac
     9481 aaaaagataa aactcaggcg gtcgaacgac cgcctggaga tgttcactta cgcctggtgg
     9541 ccgttgatgt aatcaatggc agcctgaacg gtggtgattt tctcagcttc ttcgtccgga
     9601 atctcagtat caaactcttc ttccagagcc attaccagct caacggtgtc aagagaatcc
     9661 gcgcccaggt cttcaacgaa agaagcattg ttggtaactt cttcctgctt aacgcccagc
     9721 tgttcgccga taattttctt aacgcgttct tcgatagtgc tcatactctt aaatttccta
     9781 tcaaaactcg ctttcgcgat ggttttcgta gtgtataaaa tgttgaaaaa tttgcaacta
     9841 aatcccggca ggtcttacca cgattttacg ttattttgag gccttacccc ccaataacgc
     9901 aaatcatttt gcactaatta ttactcataa ccacgcagac tgcgcgcaac atgagcattt
     9961 tgtgcaaatc gcggtcagac catgtacatc ccgccgttca catgcaaagt ttcacccgtg
    10021 atgtaagctg cttcgtcgga tgccaggaat gcaaccgcgt tggcgatttc ctgtgcgccg
    10081 ccgaggcgac ccgcaggaac ctgcgccagg atacccgcac gctggtcatc gctcagcgca
    10141 cgtgtcatgt ccgtttcaat aaagcccgga gcaacaacgt ttacagtaat accgcgtgac
    10201 gcaacttcgc gcgccagtga tttactgaag ccgatcaagc ccgctttcgc cgcagcgtag
    10261 ttggcctgac cgccatttcc catggtacca accacagaac cgatagtgat aatacgacca
    10321 tgacgctttt tcatcatagc gcgcattacc gcttttgaca gacggaaaac agatgaaagg
    10381 ttggtttcga taatatcgtt ccactcttca tctttcattc gcattaacag gttatcacga
    10441 gtgataccgg cattattgac caggatatcc acttcaccaa attctgcgcg aattttttcc
    10501 agaacagatt cgatagatgc cgggtcggtc acattcaaca tcagaccttt gccgttagca
    10561 cctaaataat cactgatcgc ctgagcgcca ttttcactgg tcgcagtgcc aataactttc
    10621 gcgccacggg ctgcgagcgt ttcagcaatt gcgcggccaa ttccgcggct tgcaccggtt
    10681 accagtgcga tttttccttc aaaattcatg attttcctct tttaaagctc gagcgccgct
    10741 gccatcgctg aaggttcgtt cagcgccgag gcggtcaggg tgtcgacaat gcgtttcgtc
    10801 aggccagtta gcactttgcc cgggccgact tcatagagat gttctacgcc ttgcgctgcc
    10861 atgtactcaa cagacttcgt ccactgaacc gggttataca actgacgtac cagtgcgtca
    10921 cgaatggcat caccattggt ttcgcatttc acatcaacgt tattcacaac aggaactgtt
    10981 ggtgcgttaa aggtgatttt cgctaattct actgccagtt tgtcggctgc tggtttcatc
    11041 agcgcacagt gagacggtac gctcactggt aacggcagcg cgcgttttgc gcccgcagct
    11101 ttacaggcag cgccagcacg ctcaaccgcc tctttatgac cggcaataac cacctgtccc
    11161 ggagagttaa agtttaccgg agaaacgacc tgaccttctg cagcttcttc acacgctttc
    11221 gcaatagacg catcatccag accgatgatt gccgccatag cgcccgtgcc ttctggtacg
    11281 gcttcttgca tgaacttgcc gcgcatctca accagacgca ccgcatcagc gaaatcaatc
    11341 acgccagcgc aaaccaacgc ggagtattcc cccaggctgt gaccggccat cattgccggt
    11401 gctttaccgc cctgctgctg ccatacgcga tacagcgcaa cagatgcagt caacagcgct
    11461 ggctgggttt gccaggtttt attcagttct tcagctggcc cctgctgggt cagcgcccac
    11521 aggtcgtagc ccagcgccgc agaagcttca gcaaacgttt cttcgacaat tggatagctc
    11581 gccgccatat cagccagcat tccaacggtt tgagaaccct gtccagggaa cacaaatgca
    11641 aattgcgtca tgttttaatc cttatcctag aaacgaacca gcgcggagcc ccaggtgaat
    11701 ccaccgccaa aggcttcaag cagaaccaac tgccccggct taatgcgccc gtcgcgtaca
    11761 gcttcatcca gcgcgcacgg gacagaggcc gcagaggtat taccgtggcg atccaacgtc
    11821 accacgacat tgtccataga cataccgagt tttttcgccg ttgcactgat aatacgcagg
    11881 ttagcctgat gcggaaccag ccagtccagt tgagaacggt caagattatt cgccgccagc
    11941 gtctcatcaa cgatgtgcgc cagttccgtt accgcaacct tgaagacttc gttgcccgcc
    12001 atcgtcagat gaattgaatt ctctggattc acgcggtcgg cgtttggcag cgtcagcaat
    12061 tcaccataac taccgtcggc atgcagatgg gtggagatga ttcccggctc ttcagaggca
    12121 gccagcaccg cagcgcccgc gccatcgcca aaaataataa tagtcccacg atctgttgga
    12181 tcgcaggtgc gcgccagtac atcggaaccg acgaccagag catacttcac cgccccagat
    12241 ttcacgtatt gattggctac gcttaatgca taggtgaaac ctgcgcaggc tgctgcaacg
    12301 tcaaatgccg ggcaaccttt aatgcccaac atgctttgaa tctgacaagc tgcgctcggg
    12361 aaagcgtgcg tagcagaagt cgttgccaca acgatcaggc caatctggtc tttctcaatg
    12421 cccgccatct caattgcgcg tgtcgccgct tcaaagccca tggttgaaac ggtttcgttt
    12481 ggcgcggcaa tgtggcgttc gcggataccg gtacgagtga caatccactc gtcagaggtg
    12541 tccaccattt tttccaaatc ggcgtttgtc cgcacttgtt cgggcagata gctgccagta
    12601 ccaataatct tcgtatacat gtacgctcag tcacttttcg gttatatacc gtcacttgca
    12661 aactgcgagt tcgctggcag cgtcctgcta ccgcagagtt ccgcttttgc caccgtccag
    12721 cagctcaaaa ccagctgggt atacagattc caggcgagcg gcaattcgct gaggaacttg
    12781 tcgctgcacc gcctgcactg cctgttcaat cgcgaccgca aaagctcgct gattggctgc
    12841 accatgactt tttatcaccg tgccgcgcaa tcctaacaga caggcgccgt tatactggtc
    12901 ggggttgagg tgactgaatc gcctcgtcag gctcttttgt agccaacgct ttaataacag
    12961 tagccaccac gaccgttttt tcccttcacc ctgagatttc agcagagaaa ggaacatcct
    13021 gacaacacct tccatcgtct ttaacgtgac atttcctgta aagccgtcac aaaccagcac
    13081 atctgtcttg ccagttaaca actcattggc ttcaagatag ccgatataat tgatagaagg
    13141 gattgtttta agcaccgctg aggcatcccg aatactgtcg agacccttta cttcttcttc
    13201 accaatattg agcaacgcca cgcgaggatt gggaatttcc accacctctt cagccagaac
    13261 tgagcccata atggcaaatt gcaccaacat tgtgctgtca caatcgacgt tggcccctaa
    13321 atcgaggacc accgttttgc ccttttgctg atgtggtaat accgtcacca gcgccggacg
    13381 ctcaatcccc tccaggggcc tgagtaataa ttttgccagc cccatcagtg ccccggtatt
    13441 accggcactg acacaggctt gcgctcgacc ttctttcacc agctccaggg ccatacgcat
    13501 tgaactccca cgactagcgc ggatagcttg cgaaggccgg gcatcactgg cgataactga
    13561 ctgcgcagga ataatctgca gacgcgaacg ttgttcaaag tcagctttag caagtaatgg
    13621 cgtgatggcg tcgggattgc cgactaaaag aagagtgagt tgcgaattag aattcagtgc
    13681 ctgcaatgct gcaggcactg tcacggaagg gccaaaatcc cctcccatga catctaacgc
    13741 cagggttaga cgtgtcaagg tatcgtcgcc gcccggtttg gtctttcccc agttgcctgg
    13801 ggaaaatcct cactaagctt catcacgcag acgcgtgatt acttagcgat gaccttgcgg
    13861 ccgcggtagt aaccgtcggc agtgatatgg tgacgcaggt gtttttcacc agaagttttg
    13921 tctacagaca ggctggtgac tgcggtcagc gcgtcatggg aacgacgcat gccacgtttg
    13981 gaacgggttg gtttattctg ttgtacggcc atggacctta ctcctcaatt acggtttatc
    14041 cccgatccaa cggggagcac caattactta cgctttaagc tggctaatac ggcaaatggg
    14101 tttggctttt gcacttcttc aggcagttca ccaaagacca tgtccgcttc ggacacttca
    14161 cagtgttcag aatcatgcac cggaactacc ggcaaggcga ggatgatttc atcttcaacc
    14221 attgcaagca gatcgatttc accgaattcg ttaacctcaa tcggttcata cgcttccggc
    14281 agtgcttcag cctgttcgtc tgaacgcaca ggactaaaac aatacgttgt gtagacctga
    14341 tgagtaaacg gcttcccgca acgctgacac tcgagcgtta ccgtcacctt cgcatcgccg
    14401 tttaacactg cgagacgttg gttatcgata gcgaacgaca tggagcattc cacatcactg
    14461 tccacactga ctacggattc ggcgacgcgc tcaacctgat caggggtata gataccctgg
    14521 taatcaaggc gtttttgagc cgtacgaacc ggatcgagag tcaggggtaa ttttaccttt
    14581 tgcatagggc gcgcatatta actttgtaac gtcatagagt caaagaaaaa ggcagcctgt
    14641 ggttgccttt tgccaataat tcgcacacat tgcgggttac tgctttattt tcgttcagtg
    14701 ctgataacga acatcaacct gcaaaacgaa gcgcatagtt taaaatggct atcatcaata
    14761 cgctatatct gatggaaaaa aatatgccta aacttatttt agcctccacc tcgccatggc
    14821 gtcgcgctct tctggaaaaa ctgcaaatct cttttgaatg tgcagcacca gaggtcgacg
    14881 aaaccccacg cagcgacgaa tcaccgcgac agttggtgct tcgactggca caagaaaaag
    14941 cgcaatctct ggcgtcacgt tatccggatc atttaattat tggttcagac caggtatgtg
    15001 ttcttgatgg cgaaatcacc ggtaaaccgt taacggaaga aaatgcccgt ctgcaattac
    15061 gcaaagccag cggcaatatc gtcaccttct atactggact ggcgttgttt aattcggcga
    15121 atgggcatct acaaacagaa gtggagcctt ttgacgtcca tttccgtcac ctgtgcgagg
    15181 cggagattga taattacgtg cgtaaggagc atcccctgca ctgcgcgggt agctttaaga
    15241 gtgaaggatt tggcattacg ctgtttgagc gtttagaggg acgtgaccct aacacgctgg
    15301 ttggtttacc gctaatcgcg ctgtgtcaga tgttacgtcg ggaagggaaa aacccgctga
    15361 tgggatagtt gtttgcgccg gatgcggtta cgtcgcatcc ggcaatcggt gcaggcggcc
    15421 tgatgcggcg taaacgcctt atcaggccaa ccgcccatta tcaggcttat atgcttgtat
    15481 tagcgcgcgt tacgcagctt ttgcaaacaa cgcttcaaac cttcatccat cggcgcttcg
    15541 atacgcatca cctcaccggt ccccggatgg gtaaacttca acgctgcggc gtgcaggaac
    15601 agacgattta atcccgtgcc tgcttcagtg agctgtctgt caaattcacg gtcaccgtag
    15661 cgatcgtcaa aggcaatcgg atgacccgca tactgtgtat gcacacggat ctgatgagta
    15721 cgtccggtta ccgggctaca acgcaccagg gtggcaaatg catagcgttc ttccacttta
    15781 aagcgtgttt ccgacggttt gccttcctga ctgacacgca cgatacgttc gccgctttgc
    15841 agaatatttt tcaataacgg cgcttgaacg ctcttcacat gcgactgcca ctgactgcgc
    15901 accagcgcca ggtaatcttt ttgcattcct ttttcacgta attgctcatg cagagaacgc
    15961 aacgccgagc gttttttcgc taccagcaac acacctgaag tgtcccggtc gagacgatga
    16021 accagttcaa ggaaccgcgc ttccgggcgc aacgcccgca aaccttcgat aacgccgaag
    16081 cttaaaccac tgccgccatg taccgccgta ccggaaggtt tattcagcac caggatatga
    16141 tcatcttcat ataagatgac gtccgccagc gccgccacct tttgcagatg tggcgaaacc
    16201 gcctcttctt cccgctcagc aacgcgaacc ggtgggatac gcacctcatc acccgcttcg
    16261 agtttatatt caggcttaat acgttttttg ttcacccgca cttcgccttt acgcaaaata
    16321 cggtaaatca tactttttgg tacgcctttc aattgggtac gcaaaaagtt gtcgatacgt
    16381 tgccccgctt cgtcagcggt gatagcaaca atttttacgg atggagtctc tgttttcatg
    16441 gtgggcgatt ctaattagcc aacaggattc gcgccactca tttttctatg cttatattta
    16501 gttttacacc ttattacttc actacgtgat cactttattg atggttatta aaccaatcac
    16561 cagcaagaag tg
//
LOCUS       DAJVUJ010000092        16538 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.092, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000092 DAJVUJ010000000
VERSION     DAJVUJ010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16538
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.092"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(56..220)
                     /locus_tag="OQQ48_002630"
                     /pseudo
     CDS             complement(56..220)
                     /locus_tag="OQQ48_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280578.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
     gene            complement(299..393)
                     /locus_tag="OQQ48_002631"
     tRNA            complement(299..393)
                     /locus_tag="OQQ48_002631"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(357..359),aa:Sec,seq:tca)
     gene            686..2068
                     /locus_tag="OQQ48_002632"
     CDS             686..2068
                     /locus_tag="OQQ48_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HCS1953348.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVLGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            2078..4369
                     /gene="yicI"
                     /locus_tag="OQQ48_002633"
     CDS             2078..4369
                     /gene="yicI"
                     /locus_tag="OQQ48_002633"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HCS1953349.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGQDTNNQRNYMFERLDLGVGETVYGLGERF
                     TALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQSVSFEVGSEKVSK
                     VQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTSFTTNYDEAT
                     VNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRRLKAKGLKIC
                     VWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNPDACKWYADK
                     LKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAHIYNELVWNVLKDTVGEE
                     EAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFWSHYIGGFEN
                     TAPAHVYKCWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLKCRMMPYLYR
                     EAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAGDVQFYLPEG
                     RWTHLWHNDELDGRCWHKQQHSFLSLPIYVRDNTLLALGNNDQRPDYAWHEGTAFHLF
                     NLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRNVVKVNGLQD
                     GSQAESEQGLVVKPQGNALTITL"
     gene            complement(4422..6131)
                     /locus_tag="OQQ48_002634"
     CDS             complement(4422..6131)
                     /locus_tag="OQQ48_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCS1953350.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPKAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDAHLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLLMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTTLDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGIVSSGEVTSAE
                     LVQ"
     gene            complement(6252..7643)
                     /gene="xanP"
                     /locus_tag="OQQ48_002635"
     CDS             complement(6252..7643)
                     /gene="xanP"
                     /locus_tag="OQQ48_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HCS1953351.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKTWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITSTSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            7923..9128
                     /gene="gltS"
                     /locus_tag="OQQ48_002636"
     CDS             7923..9128
                     /gene="gltS"
                     /locus_tag="OQQ48_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HCS1953352.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            complement(9162..11243)
                     /gene="recG"
                     /locus_tag="OQQ48_002637"
     CDS             complement(9162..11243)
                     /gene="recG"
                     /locus_tag="OQQ48_002637"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCS1953353.1"
                     /translation="MTGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFEPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(11249..11938)
                     /gene="trmH"
                     /locus_tag="OQQ48_002638"
     CDS             complement(11249..11938)
                     /gene="trmH"
                     /locus_tag="OQQ48_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HCS1953354.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            complement(11945..14053)
                     /gene="spoT"
                     /locus_tag="OQQ48_002639"
     CDS             complement(11945..14053)
                     /gene="spoT"
                     /locus_tag="OQQ48_002639"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HCS1953355.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(14072..14347)
                     /gene="rpoZ"
                     /locus_tag="OQQ48_002640"
     CDS             complement(14072..14347)
                     /gene="rpoZ"
                     /locus_tag="OQQ48_002640"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCS1953356.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            complement(14402..15025)
                     /gene="gmk"
                     /locus_tag="OQQ48_002641"
     CDS             complement(14402..15025)
                     /gene="gmk"
                     /locus_tag="OQQ48_002641"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCS1953357.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     DEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            15283..>16538
                     /gene="ligB"
                     /locus_tag="OQQ48_002642"
     CDS             15283..>16538
                     /gene="ligB"
                     /locus_tag="OQQ48_002642"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HCS1953358.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEIFRQQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQDDSDTLNSL
                     GVFVWAWPDGPQLMSDRLKELATAGFTLTQTYTRAVKNADEVARVRNEWWKAELPFVT
                     DGVVVRAAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     SVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLV"
BASE COUNT         3754 a         4228 c         4496 g         4060 t
ORIGIN      
        1 agtcgtataa aaaagggtgt tatggagcgt gtaaaacaac atcttcacct taatggcgat
       61 acagttggct gagagccagc agggggcaaa tcacctgcaa ataaacacgg cacatgctgg
      121 cgcgcatatc gcggatgcgt tcgaggacag aagaaccgac aggcggattc ttcagccgct
      181 catcgtccat cacaaaacct tagaccaggt attcctgaag ccgattgaat attgagcagt
      241 agaaaatatc tggattgaca gtattacaag gggttggaga acgtcagaag aggcattttg
      301 gcggaagatc acaggagtcg aacctgtccg ggaccgctgg cggccccaac tggatttgaa
      361 gtccagccgc ctcaccggag acgacgatct tccgcgcctc gattgctaca tggaggcggg
      421 gcgcattata gctacttcct tgagtttcta catcccccag atcagttttt tccgcctaat
      481 acacgcagcg tattatcacg aaagcattat agttcatata aaacccatat tttacaatga
      541 catatgagcg gaattaacgc taccacgatc acataaaaca agaatcataa attaataacc
      601 agatatcgga atattcgctc tcacagggat ggtttacaaa atgcgttatc ggtctcagca
      661 cccctatagc atcaaggaaa agcagatgaa gagtgaagtg ttgtccgtta aagagaaaat
      721 tggttatggc atgggagacg ccgccagcca cattattttc gataacgtaa tgttatatat
      781 gatgttcttt tataccgata tttttggcat tcctgccggt tttgtcggaa ccatgttttt
      841 ggtcgctcgt gcactggatg cgatttccga tccttgcatg gggttgttgg ccgatcgaac
      901 gcgctctcgc tggggtaaat ttcgtccgtg ggtactgttt ggcgcactgc cattcgggat
      961 cgtctgtgta ctggcctata gcacgccaga tctcagtatg aacggcaaaa tgatctatgc
     1021 agcaattact tacaccctac ttaccttact ttataccgtc gtcaatatcc cttactgcgc
     1081 attgggtggt gtaatcacca atgacccgac tcagcgtatc tcgctgcaat cctggcgttt
     1141 tgtgctggcg accgcgggag gcatgctttc tactgttctg atgatgccac tggttaattt
     1201 aattggcggt gataataaac cactcggttt ccagggcggt atcgcggtcc tttccgtggt
     1261 ggcattcatg atgctggcat tttgtttctt caccactaaa gaacgcgttg aagcaccacc
     1321 tacaacaacg tctatgcggg aagatttacg tgatatctgg caaaacgacc agtggcggat
     1381 tgtcggttta ctaaccattt tcaatatcct ggcggtgtgc gtactcggtg gggcgatgat
     1441 gtattacgtc acatggattt tgggcacgcc ggaagtgttt gtcgcttttc tcaccactta
     1501 ttgcgtgggt aacctgattg gttccgcact ggcaaaacct ctgaccgact ggaaatgtaa
     1561 agtcactatc ttctggtgga cgaacgccct gctggcagtg attagcctcg cgatgttctt
     1621 tgttcccatg caggccagca tcactatgtt tgtcttcatc ttcgtgattg gtgtgttgca
     1681 tcaactggtg acacctatcc agtgggtaat gatgtccgat accgtcgact acggcgagtg
     1741 gtgcaatggt aaacgcctga ccgggatcag ttttgctggc acgctgtttg tgctcaaact
     1801 ggggttggcc ttcggcggcg ctcttatcgg ctggatgctg gcttatggcg gatatgatgc
     1861 ggcagaaaaa gcgcagaaca gcgccacgat tagcatcatt attgcgctat tcacgattgt
     1921 tccggcgatc tgttatttgc tgagcgcgat tatcgctaaa cgctactact cactcacgac
     1981 gcacaatctg aaaaccgtta tggaacagct ggctcagggt aaacgccgtt gccagcaaca
     2041 attcacctct caagaagtgc agaactaagg aacggcaatg aaaattagcg atggaaactg
     2101 gttgattcaa cctggcctca atttgattca cccgcttcag gtgttcgagg ttgaacagca
     2161 ggataatgaa atggtggtct atgctgcccc ccgtgatgtg cgtgaacgta cctggcagct
     2221 tgatacgcct ttatttacgc tgcgcttttt ctccccacag gaaggtattg tcggtgtgcg
     2281 gattgagcat tttcagggag cgctgaataa cggtcctcat tatccgctca atattttgca
     2341 ggacgtgaag gtcacaatcg aaaacacaga acgttatgct gagtttaaaa gtggcaactt
     2401 aagcgcgcgt gtcagcaaag gtgagttctg gtcactcgat tttctgcgca acggtgaacg
     2461 tattaccggt caggacacga ataatcaacg aaattatatg tttgagcggc ttgatcttgg
     2521 cgttggcgaa acagtttatg gtctgggaga gcgttttact gccctggtgc gcaatggtca
     2581 gacggtagag acctggaacc gggacggcgg cacaagtact gaacaggcgt ataaaaatat
     2641 cccgttctat atgactaacc gtggctatgg ggtgctggtc aatcaccccc agagtgtctc
     2701 ttttgaagtg ggatcggaga aagtctccaa agtgcagttc agcgttgaga gcgaatatct
     2761 cgaatacttt gttatcgacg gtccgacgcc gaaagcggta cttgatcgtt atacccgttt
     2821 tactggtcgt ccggcgctgc cgcccgcatg gtccttcggc ctgtggctaa ccacttcatt
     2881 taccaccaac tacgacgaag cgacggtaaa cagctttatc gatggtatgg cggaacgcaa
     2941 tctgccgctg catgttttcc actttgactg tttctggatg aaagccttcc agtggtgcga
     3001 ttttgagtgg gacccgctga ctttcccgga cccggaaggg atgatccgcc gcctgaaagc
     3061 gaaagggctg aaaatctgcg tctggattaa cccctatatc ggtcaaaaat cccccgtctt
     3121 taaagagtta caagagaaag gctatttact caaacgcccg gacggttcgc tatggcagtg
     3181 ggataaatgg cagccaggtc tggcgattta tgactttacc aatccggatg cctgcaaatg
     3241 gtacgccgac aaactgaaag gtctggtcgc gatgggcgtt gattgcttta agaccgactt
     3301 tggcgaacgt atcccaactg atgttcagtg gtttgacggt tccgatccgc agaaaatgca
     3361 taaccattat gcgcacatct acaacgaact ggtgtggaac gtgctcaagg acaccgttgg
     3421 tgaggaagaa gctgtcttgt ttgcccgctc ggcctccgtc ggtgcgcaga aattcccggt
     3481 acactggggt ggcgattgtt acgctaacta cgaatcaatg gcggaaagcc tgcgcggtgg
     3541 tttgtctatt ggcctttcag gttttggctt ctggagccac tatatcggcg gctttgaaaa
     3601 taccgctccg gcgcacgttt acaaatgctg gtgcgcgttt ggtttgctct ccagccatag
     3661 ccgtttacac ggtagcaaat cttatcgtgt gccgtgggcc tacgatgatg agtcctgtga
     3721 tgtggtgcgc ttcttcacgc aactgaaatg ccgcatgatg ccgtatctgt atcgtgaagc
     3781 tgcgcgtgca aacgcgcggg gtacgccgat gatgcgggcc atgatgatgg agttcccgga
     3841 cgatccggct tgtgattacc ttgaccgtca atacatgtta ggcgacaacg tgatggttgc
     3901 gccggtgttc actgaagcgg gcgatgtgca gttctacctg ccggaaggtc gctggacaca
     3961 cctgtggcac aacgatgagc tcgacggtcg ttgctggcat aaacagcagc acagcttcct
     4021 gagtctgccc atttatgtgc gtgataacac cctactggcg ctgggcaaca acgatcaacg
     4081 tcccgattac gcgtggcacg aaggcacggc attccacctc tttaatctgc aagacgggca
     4141 tgaagccgtc tgtgaagtgc ccgctgctga cggatcggtg atctttactt taaaagcagc
     4201 acgtactggc aacacgatta ctgtgactgg tgcgggcgag gcgaagaact ggacactgtg
     4261 cctgcgcaat gttgtgaaag taaatggtct gcaagacggt tcgcaggctg aaagtgagca
     4321 ggggctggtg gtgaagcctc aagggaatgc gctgacaatt acgttgtaat ctattgatgg
     4381 aatgccgggt acgtctgagt cgtacccggc gagattgatg tttactgcac cagctctgcc
     4441 gacgtaactt caccgctgga aacgatgcca gcattcatgg tttgagtcac ttgctgcttt
     4501 gcggcgttca cggcatgcaa ttgcccgcta accgtaggtt tcagcggggc attggcctga
     4561 atatctccac tggcggtaag ctgaatattg ccgtcgccag tcaacggtaa ttcaggccat
     4621 ccccattgtt gcaaaatatt caccggcaca ccgcgtccat tcaggctgat atgggtctga
     4681 cgttgtggcg tttgtgaaac actggcagtg gcttccagaa tgcctttttc agtaaatgca
     4741 ctcagttcgc tgatattcac cgtgctgctg ttggcggtca gcgccagcga cgggcgacga
     4801 acatcaacac gattgaatgt cgcggcggcg gcattcagat tcgccgagcc actccagacg
     4861 ccccatttat gatcggtaac cagcgtcagg ttggcaccgt aaccatcgag cgtggtgagc
     4921 tgccacggga agtcagggtc gatatcaatg atcagattgc ggctggcgct aaatctcttc
     4981 agttgcaggc tgtttaacca accgggtgtc gtttccatcc acaactgttg ccagtttttc
     5041 ggcaaggtat attccagccc ggcaattgcc gcatcatcaa ggatcaacgt tttcccgtca
     5101 cgcagccagt tccctgacgt tctgaccata cccccttccc agcggctggt gaactggcgc
     5161 agcgctacgc cctgcgggga aaattccgcg tttataatcg ggtcaaataa atgcagcgaa
     5221 ccataaatga actcgctagc gttcatcaac agtttgcctt cttgtgtctg ccagtcatct
     5281 ttactgaagg tcatgttgcg caagctgaga tcgaggtcgg tcaccgccca gtccggacct
     5341 tgcaaatgag catcgatcac ttccaggcga ccaatctgca acgacgggac agagcgtaat
     5401 ggcgcaaaga agtcggttag cgatttttcg ctttgtagac gtatatccgc catgcgcagg
     5461 ttttccactt gccagctgcc gtcggcgtta cgctgcgcgt ttccggttaa tgtcccgcgg
     5521 gcgatgtcgg cacccaggtt agtcagcgta acgcgatcgt tatcaatact gccttcaatc
     5581 agtacattgg tggcaggaac atcattgagc gaaagcgatc cggcactaaa ctgaatctgc
     5641 gccttcgtac ccagcacttt accggctttt ggtgaccacg gaactacgcc gccatttacc
     5701 cgctgcgcgc tcagtttcca ttcgctattc gggctattaa acgccatatc acgcagttgc
     5761 agacgatcgg ctttgaacgg tagcggcgcg gtctggtcgg tgagattcag cgtcccgttt
     5821 tccagcagga tggtatcgac atggcgtggt tcggtcagtt gccgactgct tagcgcaatg
     5881 tcgacacttt tggccaccag ggtcgcgggc tgaccatcac gaccaaacgt gacattctcc
     5941 agcacgatat gagatggcgc ggaaaaacgg tgatccatcg ccccgaaggc cagatgatag
     6001 tcgctattct cggaaaccca tgcgctgata tgttctgctc cccagcgggt ttgcagaaga
     6061 aaatagaggc cagcgatcac cactaacaga gcgatgagaa tgtagagaag cagcttccca
     6121 ataaatttca tggtcttcca ttccgtgaaa tgcacataag agagttatgc acgatttacg
     6181 cgcaatcctc aaggcgggaa tggtgaaaga ggtgagactg cggcggtaat tatcttaccg
     6241 ccgccagtga attactgttt ttctggtggg aaaatcagat tcagcacgat ggcagtaata
     6301 ccgcccgcgg cgatcccgga ggagagcagg tttttcagcc attcaggggc aaactgcaaa
     6361 atcagcggct gctgagacac gcccagacca accgccagcg acagcgcgat aatcagaatc
     6421 gcccgacggt tcagcggctc acgagaaacg atacgcacac cggaggcggc gatggtgcca
     6481 aacattacaa gcgttgcgcc gcccagaacc ggttctggaa tgtgttgtac aaaaccgctc
     6541 actgccggga acagacccag cacgatcaac atcagcgcga cgacaaaacc gacatagcgg
     6601 ctggcaacac cagtcaactg gatcactccg ttgttttgcc cgaagcagga gttcgggaag
     6661 gtgttaaaca ccgccgaaac aaacgagttc aggccgtttg ccagcacgcc gcctttcagg
     6721 cgtttcatgt acagcggacc ggacactggc tgttcggaaa cgtcagaggt cgacgtgata
     6781 tcgccaatgg tttccagcga agtgatcata aagaccagca tcagcggcag cagcagactc
     6841 cattcaatgc caagaccgta atagagcggc gttggcacca taatcagttc ttgcgtcatc
     6901 ggttcgttgc tttctggcaa catgcccata aaccacgcca gcgcatatcc ggccgccatc
     6961 gcaatcacca gtgaggccac gcgtaagtaa gggttacgtt gacggttaag caggataatt
     7021 aaggctaaga ccacgcctgc cagcagcaga tttttcggtg cgccgaaggt gttatcgctc
     7081 atggctgcgt aaccgccgcc aatggacgtt aacccaacct gaattagcga caggccgata
     7141 atcatcacca caacgccaga aaccagcggc gtaataatgc ggcgcgccag atgcagaacg
     7201 cgggagatca ccatctcggt gcaacttgcc agcatcaacg tgccgaacaa agccgccatc
     7261 atggtaggaa catcagcacc accggttttc agcgcggtac cgcccataat cagcggggca
     7321 acaaagttga agctggtgcc ctgaatagac aacagcccgg agccaaccgg accccaggtc
     7381 ttaatttgaa taatcgatgc cacaccggag gcaaacagcg acatactaat aatgtgttgc
     7441 gtgtcttgtg ccggtaaacc cagcgcctgg cagattaata gcgctggcgt gatcaccgca
     7501 acgaacatcg ccagcagatg ctgacaggcg gcaaacaggg tttgaggaag cggcggacga
     7561 tcttcaagac ggtaaatcag ttcgctgttt tgagtctgcg caaccggttg cgcattttct
     7621 gactcgaggg tggaaacaga catcgaaaac aatcccgtgg tagcaaagcg ggcattttag
     7681 ctgaccgaaa gagaaaagca aacgtttgcc acatcctcat ctaaatattt aaatgagata
     7741 aatgcacttt tttatataac ttttggtatt tttctgctta aaatccatgc catttgttca
     7801 ttaacagctg agaatttgac ccaggaaagc ataactcgat acccgtcata gttcatgaag
     7861 ttgccactgc gcgatgaagt atgacgagta tgaaagagtg atgcggatac aaaggagtaa
     7921 ctatgtttca tctcgatact ttagcaacgc ttgttgccgc aacgctgacg ttgctgctcg
     7981 ggcgtaagtt ggtccattcc gtctcctttt tgaagaaata caccataccg gaacctgttg
     8041 cgggtggttt gttggtggcg ctggcgctac tagtactgaa aaaaagcatg ggctgggaag
     8101 tcaactttga tatgtccctg cgcgatccgt taatgctggc tttcttcgcc accattggcc
     8161 tgaacgccaa cattgccagt ttgcgtgccg gtgggcgtgt ggttggcatc ttcttgattg
     8221 tggttgttgg tctgttggtg atgcaaaatg ccattggcat tggtatggct agcctgttag
     8281 ggcttgatcc gctgatgggg ctgttggccg gttctattac gctttccggc ggtcacggta
     8341 cgggcgctgc gtggagtaaa ttgttcattg aacgttatgg cttcaccaat gcgacggaag
     8401 tggcgatggc ctgtgcaacg ttcggtctgg tgctgggcgg cttgattggc ggtccggtgg
     8461 cgcgctatct ggtgaaacac tccaccacgc cgaacggtat tccggatgac caggaagtcc
     8521 cgacggcgtt tgaaaagccg gatgtgggac gcatgatcac ctcgttggtg ctgattgaaa
     8581 ctatcgcgct gattgctatc tgcctgacgg tggggaaaat tgttgcgcaa cttttggctg
     8641 gcactgcttt tgaactgccg accttcgtct gtgtactgtt tgttggcgtg attctgagca
     8701 acggtctgtc aataatgggc ttttaccgcg tctttgagcg tgcggtatcc gtgctgggta
     8761 acgtaagctt gtcgttgttc ctggcgatgg cgttgatggg gctgaaactg tgggagctgg
     8821 cttcgctggc gctgccgatg ctggcgattc tggtggtaca gaccatcttc atggcgttgt
     8881 atgccatctt cgttacctgg cgcatgatgg gcaaaaacta cgatgcggca gtgctggctg
     8941 cgggtcactg tggttttggc ctcggtgcaa cgccaacggc aatcgccaac atgcaggcga
     9001 tcactgaacg ctttggcccg tcgcacatgg cgtttttggt ggtgccgatg gtcggtgcgt
     9061 tctttatcga tatcgtcaat gcgctggtaa ttaagttgta tttgatgttg ccgatttttg
     9121 ccggttaacc gatgaagcgg cggtcttctc accgccgcct tttacgcatt cgagtaacgt
     9181 tctgtctccg gcatccagcg ttctatcaag gcttttgcct gttgtgggta acgttcgtga
     9241 atatgtcgcg ccaggcgctg aacttccggg atcatcgcct gatcgcgcag taaatccgcc
     9301 actttaaatt cagcattacc cgtctgacgc gtgcctaaca attcgccagg gccgcgaatc
     9361 tccagatctt tttgcgcaat cacaaaaccg tcgttactgt cgcgcagcac ttgcaggcga
     9421 atttgcgccg ttttagaaag cggcgttttg tagagcagca cgcagtgaga agccaccgcg
     9481 ccacgaccta cgcgcccgcg cagctggtgt aactgcgcca gacccagacg ctcagggttt
     9541 tcgataatca tcaggctggc gttaggcaca tcaacaccga cttcaataac ggtagtggca
     9601 accagcagat gtagctcacc ttgtttaaac gacgccatca ccgcctgttt ctcggctggt
     9661 ttcatccgcc cgtgtaccag gccaacattc aattctggta gcgccagttt caactcttcc
     9721 caggtagctt ccgccgcctg cgcttccagt aactctgact cttcaatcaa cgtacaaacc
     9781 cagtacgcct gacgaccttc agttatgcag gcatggcgca cgcggtcaat gatgtcggta
     9841 cggcgggtat caggaatagc gaccgtagtc actggcgtgc ggcctggcgg cagctcatct
     9901 atcaccgagg tatcgagatc ggcatacgcc gtcattgcca gcgtgcgggg gatcggcgtg
     9961 gcggtcatga tcaactgatg cggatggaag ccttgctgct ggcctttctc ccataacgcc
    10021 agacgctgat gcacgccaaa gcgatgttgt tcgtcgataa tcaccagcgc cagaccgtta
    10081 aactgcacct gttcctggaa gatggcgtgc gtaccaacaa tcatctgcac ctgaccgctg
    10141 gcgatggctt cctgctgaga cagtcgcgct ttgcctttct gcttaccggc gagccagccc
    10201 acttcgatac cgagcggttc aaaccagttg cggaagttat tggcgtgctg ttcggcgagt
    10261 aattcggttg gtgccatcaa tgccacctgt ttgccgtggg caatcgcacg cagcgcggcg
    10321 agggcggcga ccagcgtttt accggaacct acatcgccct gtaccaggcg catcatcggc
    10381 acatccagcg ccatatcgcg ctcgatctcc gccactacgc gtgcctgtgc gcccgttggc
    10441 ttgaacggta aggctgcgag gagtttattt ttcagcgcat cattggcgct cagcggctgg
    10501 gcatgaaaac gctgtgctcc ggcacgtaag gctaacatgc tgaggttgtg cgccagcagt
    10561 tcttccagaa tcagacgacg ttgcgccgga tgctgcccgg tttccagatc gctaagctgt
    10621 agcgtcggtg gcgggcggtg caaagtgcgc aacgcttccg gtagcgtcat cattccttgt
    10681 gacagttccg gcggcaggag ttcttcaatg gcgcaggtgt cgagcagatc cagcgcctgg
    10741 tcggttaatt tacgcagcgt ggcctgcttt acgccttccg ttgttggata aaccggcgtg
    10801 agcgtttcct gtaattctgg cgtgctgaga tcgccctgca cgcggtattc cgggtggatc
    10861 atctccgcac catatttacc gcgctttgct tcgccataag ccagtacacg gcggcccgtc
    10921 gccaggctat ttttcattgc cgcgctgaaa ttgaaaaagc gcatggtgag gatgccggaa
    10981 ccgtcgctga tctggcaggt catcatccgc cgaccgccga aggagatatt gcagttcagc
    11041 acttcgcctt ccaccgtggc ataaacgccc ggcagtagtt ctccgatggg gtagagatgg
    11101 gtgcgatctt cgtagcgcag gggaaggtgt aagagtagat cctgcacggt atgcaggttg
    11161 attttcgcca gtttgttact aagtgctgcg ccaacgcccg ttagggaact gagtgggaca
    11221 gcatctaaca ggcgacctgt catggcactt accctgcagc ctgcatagta gcccaccagt
    11281 cggcatcagc ttcgatctcg ccttgctgat tgacgtgggg ataaggcagg cctttgcgtt
    11341 ttgcgacttt cgccagcacc ggatagccgc cttcaaacaa caggcgttgt tgctctgctt
    11401 ccggcaacat gctgttttca cgcaggtaca tgcctgcatt ttgccgctga cgctgggctt
    11461 cgtaaagaat gagggctgag gcaacggaaa cattcagcga ctgcaccatg ccgatcatcg
    11521 gaatgatgat gtcctgatcc gccagggcca atgcttcctg cgtgatgccc gttttctcct
    11581 gtcccatcaa aatgcaggtc gggcgagtgt aatcaatttc gcggaaatcg acagcgttat
    11641 cagaaagatg ggttgccaga atctgcatgc cctggccttt gagatgagcg acggcatcgc
    11701 caatggtgcg gtgtgttttc acctgtaccc agctgttact acccgccgct gccgaagcca
    11761 tggtgcgcat gcggctacca ggccagacgg cgtgaacttc atgtacgcca acggcatctg
    11821 cggtacgaat aatcgcagaa acgttatgag gtttgtggac ctgctccatg cagacggtca
    11881 gatcaggctg ccgcctggcg agcatttcgc agatgcgtgc ataacgtgtt gggttcataa
    11941 aacattaatt tcggtttcgg gtgactttaa tcacgtctgg catcacgcgg attttgcgca
    12001 tgatattcgc cagatgcaca cggtcacgag cggtcagacg aataaaggcg ctgtagacgc
    12061 gaccatcttt ctcttccgta ttcaaacttt gaatattcga agtcgtggtg ttaattgccg
    12121 ccgtcaggtt tgccagcgca ccctgatgat tgaacatctc caccttgatt tcggtgatga
    12181 actcctgcgc cgtctcttta tcccattcca cagccataaa cttctctggc tctttctggt
    12241 agccacggat gttacggcag gattcatggt ggatcaccag acctttaccg gggctgacgt
    12301 gggcgataat cgggtcgcca ggaatagggc ggcagcattt cgcaaaggtg atcagcacgc
    12361 catccgcgcc tttgatcggc agatgcccgt ggctttgagt cgccggtgga atggaggcgt
    12421 ccccatgttg tagatttttc gcgaccacca cgctcattgc gttaccaagg ccgatttctg
    12481 ccagcagatc gtcaagcgtt gccagcttca tgcgatccag ctcgcgctga atattttcct
    12541 gcgggatttc attgagctta cggctaccac ccaaagcatg gttgagcaga cgacggccca
    12601 ggcttacaga atcatcacgc ttgaggtttt tcagcaactg acgaattttg gcgcgcgctt
    12661 tcgagctaac gacaaagttc agccaagcgg cattcgggcg agcgcccgga gcggtaatga
    12721 tttcaacggt ctgaccgcta gtaagcggct gcgacagcgg gtaaggctgg cggtcgacgc
    12781 gtgcgcccac gcaggcatgg ccgatatcgg tatgcactgc ataagcgaag tcgacgggcg
    12841 ttgcaccggc aggcagctcg acaatgcgcc cttccggtgt gaaaacgtaa atctcatccg
    12901 ggaagagatc ggatttaacg ctctcgataa attcaaacga actaccggcg ctctgttgca
    12961 gctccagcag gctttgcatc cagcgctggg cgcgtatttg tgcggtagta ctggtttcgc
    13021 cgtgctcttt ataagcccag tgcgcggcaa cacccatctc cgccatctga tccatatctt
    13081 cggtacggat ctggacctca accggcacgc cgtgcgggcc aatcatcgag gtgtgcaacg
    13141 actgatagcc gttcgctttt ggaatggcga tatagtcttt cacgcggccc ggacgcggct
    13201 tgtacaggct gtgcatctgg cccagcacgc gataacaggt gtcagaatca ttgacgatca
    13261 cgcggaaagc gtagatgtcc atgattgagt gaaaacgctg ctctttgagc accattttgc
    13321 agtaaatcga ataaagatgc ttttcgcgac cactgacgcg gcacggtatt cccgcttcct
    13381 gcaaacgccc ttcgatttca gaaagaatct tctggatcat ctctttacgg ttgccgcgcg
    13441 cggctttcac cacttctttg attacgcgat aacggttggg atacagcgcc tcaaaaccca
    13501 gctcttcgag ttcggtttta atgtggtgga tacctaaacg gtgcgccagc gggctgtaaa
    13561 tttcgagagt ttcacgggcg atgcggcgac gtttgtccgg gcgaagtgag cccagcgtgc
    13621 gcatgttgtg ggtacggtcg gcaagtttga tgaggatgac gcggatatcc tgcaccatcg
    13681 ccataatcat cttgcgaaag ttttcggcct gcgcctcttt cttatcgcgg aacttgagtt
    13741 tatcaagttt cgacaccccc tctaccagct cggcgacgct tttaccaaaa agctgttcca
    13801 tatcctggta ggtggcggga gtatcttcaa tcacgtcatg cagcagcgcc gccatcagcg
    13861 tttcatagtc gagtttcatc tcggccagaa tgcaggcaac cgctaccggg tgcgtgatat
    13921 agggttcacc gcttgaacgt gtttgcccct cgtgagcatc acgtgcaacg agatacgcct
    13981 gccgcagacg cttgatttgg tcttccggca ggtaggtttg aatcagttga ttcaggcttt
    14041 caaacagata caagggcgac ccgctttgtg attaacgacg accttcagca atagcggtaa
    14101 cggcttgtaa ttcagcggct tcctgctctt gctgttcctg gcgttcgcga acgtcgagga
    14161 tctggttgtt gatcagacct tcttcgattt cgcgcagcgc gattacagtg gttttatcgt
    14221 tttcttccgg taccagcgga tcctttccgc ctacctgcat ctgacgagcg cgacgcgcgg
    14281 cgaccagtac caggtcaaaa cggttaccaa ttttctctac agcgtcctga acagttacgc
    14341 gtgccatact taaaaagctc cacaggtgaa gaaatgactg ggcatgatac tgaaatcagg
    14401 ttcagtctgc caacaatttg ctgattaaag cgtcatgacg ctgcttttgg cggctcatgc
    14461 gcagacgttc ggcgcgaata atggtcttca aatcggtcaa cgcggtatcg aagtcatcat
    14521 tcacaatcag gtaatcatat tcgtcgtaat ggctcatttc tgcaacagct tgcgccatac
    14581 gctttgcaat gacctcttcg ctgtcctgac cgcgaccgcg tagacggcgg tccagttcaa
    14641 ttttagacgg cggtaaaata aagatactcc gcgcgtgcgg catcttctgg cgaatttgct
    14701 gcgcgccctg ccagtcgata tcgagaaaaa catcgacacc ggtcgccagt acttgctcaa
    14761 tggcctcacg cgaagtgcca tagtaattac caaaaacttc tgcgtgttcg aggaacgcat
    14821 ctctgctaat catttcttta aattcatcat gattaacaaa gaaataatgt tcaccgtgga
    14881 cttcaccagg acgcggttgg cgtgtggtgt gtgaaacaga aacctgggtg tcatacaacg
    14941 gttgggtttt taataaagcc tgaatcaggc tggatttacc cgcgccactg ggggcagaaa
    15001 caatataaag cgtgccttga gccatgagta tctttcgtat gtgattagcg aaataaagcc
    15061 tacatacgag cttattatac acggcgcggc catgtgacgt agcccttgct cacacttttt
    15121 gcgcaaagtt gttgcgtttt gcgtagctgc ttccgcattt gcctgtgatt tactgcaata
    15181 aagacgattc tcaggaagcg tcctcgcaga ctccgttttt gccactcggc tttgccttca
    15241 ccacgttgta tatctgccag cctgactaca gcggaggtga agatgaaagt atggatggcg
    15301 atattaataa gtatcttgtg ctggcaatca tctgtgtggg cggtctgtcc ggcctggtcg
    15361 ccagccagag cacaggaaga aattttccgc cagcaacagc aaataaaaca gtgggacgat
    15421 gactactgga aggaaggaaa aagtgaggtg gaagacggtg tttacgatca gttaagcgcc
    15481 cgtcttacgc agtggcaacg ctgctttgtg agcgagcccc gcgacgtgat gatgccgcct
    15541 ttaaatggtg cggttatgca tccggttgcc catacgggtg tacgcaaaat ggcggataaa
    15601 aacgcattaa gtctgtggat gcgagaacgt agcgatcttt gggtgcagcc aaaagttgat
    15661 ggcgttgcgg taaccctggt ttatcgggac gggaaactga acaaagcaat cagtcgcggt
    15721 aatggcctga aaggcgagga ctggacgcag aaagttagct taatttccgc tgtgccgcaa
    15781 accgttagcg ggcctttagc caacagtacg cttcaggggg aaatatttct ccagcgcgag
    15841 gggcatatcc aacaacaaat ggggggaata aatgcccgcg caaaagttgc tggcttgatg
    15901 atgcgccagg acgatagcga cacgctgaat tctctgggcg tttttgtctg ggcatggccg
    15961 gatggaccgc agttaatgtc tgatcgttta aaagagctgg ctaccgcagg ttttactcta
    16021 acgcagacgt atacccgtgc ggtgaaaaat gctgatgaag ttgcgcgcgt acgcaatgag
    16081 tggtggaaag cggaattacc cttcgtcacc gatggcgtag ttgtacgagc ggcgaaagag
    16141 ccagaatccc gccattggct accgggccag gcagagtggc tggtggcctg gaaatatcaa
    16201 cctgtagctc aggttgccga agtgaaggcg attcagtttg cggtgggtaa gagcggtaaa
    16261 atatcggtgg ttgcgtcact cgcatctgtc atgctggatg ataaaaaagt ccagcgggtg
    16321 aatattggtt ccgtcaggcg ctggcaggag tgggatattg cgcctggtga tcagattctc
    16381 gtcagccttg ccggtcaggg tattcctcgc attgatgatg tggtgtggcg cggtgcagaa
    16441 cgtacaaaac cgacaccgcc agaaaaccgc tttaactcgt tgacctgcta ctttgcttct
    16501 gatgtttgtc aggaacagtt catttcacgc ttagtctg
//
LOCUS       DAJVUJ010000093        16521 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.093, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000093 DAJVUJ010000000
VERSION     DAJVUJ010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16521
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.093"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            95..2323
                     /gene="crfC"
                     /locus_tag="OQQ48_002643"
     CDS             95..2323
                     /gene="crfC"
                     /locus_tag="OQQ48_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HCS1953359.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAAQLAL
                     PWSQPNIAERYAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDALNAQLAALFQD
                     DSEPLSEIRTCCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHVNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            2320..3203
                     /locus_tag="OQQ48_002644"
                     /pseudo
     CDS             2320..3203
                     /locus_tag="OQQ48_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
     gene            3467..4969
                     /gene="proP"
                     /locus_tag="OQQ48_002645"
     CDS             3467..4969
                     /gene="proP"
                     /locus_tag="OQQ48_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HCS1953360.1"
                     /translation="MLKRKKVKPITLRDVTIINDSKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            5035..5172
                     /gene="pmrR"
                     /locus_tag="OQQ48_002646"
     CDS             5035..5172
                     /gene="pmrR"
                     /locus_tag="OQQ48_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HCS1953361.1"
                     /translation="MSDAHTLLQVNGGECNEKPCLYESLTTVFSVLVVSSFLYIWFAT
                     F"
     gene            complement(5190..6248)
                     /gene="pmrB"
                     /locus_tag="OQQ48_002647"
     CDS             complement(5190..6248)
                     /gene="pmrB"
                     /locus_tag="OQQ48_002647"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HCS1953362.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRA"
     gene            complement(6249..6917)
                     /gene="basR"
                     /locus_tag="OQQ48_002648"
     CDS             complement(6249..6917)
                     /gene="basR"
                     /locus_tag="OQQ48_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HCS1953363.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKSRIRTVRGFGYMLVA
                     NEEN"
     gene            complement(6914..8557)
                     /gene="eptA"
                     /locus_tag="OQQ48_002649"
     CDS             complement(6914..8557)
                     /gene="eptA"
                     /locus_tag="OQQ48_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HCS1953364.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTV
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            complement(8661..9998)
                     /gene="adiC"
                     /locus_tag="OQQ48_002650"
     CDS             complement(8661..9998)
                     /gene="adiC"
                     /locus_tag="OQQ48_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HCS1953365.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTPGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            complement(10135..10896)
                     /gene="adiY"
                     /locus_tag="OQQ48_002651"
     CDS             complement(10135..10896)
                     /gene="adiY"
                     /locus_tag="OQQ48_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HCS1953366.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            complement(11221..13488)
                     /gene="adiA"
                     /locus_tag="OQQ48_002652"
     CDS             complement(11221..13488)
                     /gene="adiA"
                     /locus_tag="OQQ48_002652"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HCS1953367.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTTDFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     YWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHAMCVKA"
     gene            complement(13687..14595)
                     /gene="melR"
                     /locus_tag="OQQ48_002653"
     CDS             complement(13687..14595)
                     /gene="melR"
                     /locus_tag="OQQ48_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HCS1953368.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMMIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            14878..16233
                     /gene="melA"
                     /locus_tag="OQQ48_002654"
     CDS             14878..16233
                     /gene="melA"
                     /locus_tag="OQQ48_002654"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCS1953369.1"
                     /translation="MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATLLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPSTLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPHIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGESSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            16336..>16521
                     /locus_tag="OQQ48_002655"
     CDS             16336..>16521
                     /locus_tag="OQQ48_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1953370.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIM"
BASE COUNT         3987 a         4002 c         4348 g         4184 t
ORIGIN      
        1 tatttcttgc agtaattata aaagccgaaa acagaactca aaaacaatct ggctaaataa
       61 aagtaacaaa atttgcttta aggaagaatt ttctatgtac acacagaccc tgtatgagtt
      121 aagtcaggag gctgaacgcc tgttacagct ttctcgccaa cagttgcagt tactggaaaa
      181 aatgcctctc tctgtacccg gagacgatgc ggcacaactg gctttaccct ggagtcagcc
      241 taatatcgcc gaacgttacg cgatgctgaa taatgagttg cgtaaaattt cccgactgga
      301 aatggtgctg gcgattgtcg gtactatgaa agcagggaaa tcaaccacca ttaatgccat
      361 tgttggtacg gaggttctgc ctaatcgtaa tcgcccaatg actgcgctgc cgacgcttat
      421 tcgccatacg cccgggcaaa aggaaccggt actgcatttt tcacatgtcg cgccaatcga
      481 ttgtttaatt caacaattac aacagcgcct gcgtgattgc gatattaagc atctgaccga
      541 tgtgctggaa atagataaag atatgcgtgc gcttatgcag cggatcgaaa atggcgtcgc
      601 tttcgaaaaa tattatctgg gtgcccagcc tatttttcat tgtctgaaaa gtttgaatga
      661 tttagtgcga ctggcgaagg cgctggacgt cgattttcct ttttctgctt acgccgccat
      721 tgagcatatt cccgtgattg aagtggagtt tgtccatctg gcggggctgg aaagttatcc
      781 cggtcagttg acgttactgg atacccccgg gccaaatgaa gccgggcaac cgcatctgca
      841 aaaaatgctt aaccagcagc tggcacgcgc ctcggcggta ctggcggtgc tggattatac
      901 gcaactgaaa tcgatctccg atgaagaggt ccgtgaggcg attttggcgg tggggcaatc
      961 ggtgccgctg tatgtgctgg tcaataagtt cgatcaacag gatcgtaaca gtgacgacgc
     1021 cgaccaggtg cgggcactga tttccgggac gctgatgaaa ggctgtatta cgccacagca
     1081 gatatttccg gtgtcgtcga tgtggggcta cctggcgaat cgggcgcgct atgagttagc
     1141 caacaacggt aagttaccag cgccagagca acaacgctgg gtggaagatt ttgcccatgc
     1201 cgcgctcggc aggcgctggc gtcatgccga tctggcggac ctcgaacata ttcgtcatgc
     1261 tgccgatcag ttgtgggaag attcgctgtt cgcccagcca attcaggcgt tgcttcatgc
     1321 cgcttacgct aacgcctcat tgtatgctct gcgatctgct gcgcataaac tgttgaatta
     1381 cgcgcagcag gcgcgggaat acctggattt tcgtgcgcac gggttaaacg tcgcttgtga
     1441 acaattgcgg caaaatatcc atcaggtcga agaaagtttg cagctattgc aactcaatca
     1501 ggcgcaggtg agcggcgaga ttaaacatga aatcgagctg gccctgacct ccgccaacca
     1561 ctttctgcgt caacagcaag atgcgctgaa tgcgcagtta gccgccttgt ttcaggatga
     1621 ttcggagccg ttaagcgaga ttcgtacctg ctgtgagaca ctgttacaga cggcgcagaa
     1681 caccatcagt cgcgacttta cgctgcgttt tgccgagctt gaatccaccc tttgccgggt
     1741 gttaaccgat gttattcggc ccattgagca acaagtcaaa atggaattga gcgagtcagg
     1801 gtttcgtcct gggtttcatt ttcctgtttt tcacggagta gttccccacg tcaacactcg
     1861 ccagctgttc agtgaagtca tttcgcgcca ggaggcaacg gacgagcaga gcacgcgttt
     1921 aggcgttgtg cgtgagactt tttcgcgctg gttgaatcag cccgactggg gacggggaaa
     1981 tgagaaatcc ccgacagaaa cggttgatta cagtgtgttg caacgagcat taagcgcaga
     2041 agtcgatctt tattgccaac aaatggctaa agttctggca gagcaggtcg atgaatctgt
     2101 tacggcaggc atgaatactt ttttcgctga gttcgcttca tgtttgacgg aattacagac
     2161 gcgtttacgc gaaagcctgg ctctgcgtca acaaaatgaa tcggtggtca ggctgatgca
     2221 gcagcaattg cagcagactg tgatgactca cggctggatt tacaccgacg cccagctgtt
     2281 acgcgatgat attcaaacac ttttcacggc agaacgatat tgaccaagac gttactggac
     2341 ggccccggtc gcgtgctgga gtcggtttat ccccgctttt tagtggatct ggcgcagggc
     2401 gatgatgccc gccttccaca agcccatcag cagcagtttc gtgaacgact gatgcaggaa
     2461 cttctttcgc gtgtgcagct tcagacatgg acgaacggcg gcatgttaaa tgcgccgctt
     2521 agcctgcgac tgacaatggt ggaaaaactg gcgtcgatgc tggatcccgg tcatctggcg
     2581 ctgacgcaga tcgcgcagca tctggcgctg ctgcaaaaaa tggatcaccg ccagcactct
     2641 gctttcccgg agctccccca gcaaattgcc gccttgtatg agtggttttc agcccgttgt
     2701 cgctggaagg aaaaggcgtt aacgcaacga ggccaggcct actggtgcag gcaggtgatc
     2761 agagcgagca aatttttacc cgctggcgtg ctggggcgta taacgcctgg tcgttgcctg
     2821 ggcgctgttt tatcgttctc gaagagttgc gttggggggc atttggcgat gcctgtcgtc
     2881 tgggaagccc gcaagcggtg gcgttgctgc tgggtgattt gcgcgtgaaa gcgacacaac
     2941 atctggcaga gagtatcaat gcggccccga ccacgcgtca ctattaccat cagtggtttg
     3001 cctcttcgac cgttccgacg ggcggggatc atgctgattt tttaagttgg ctgggaaagt
     3061 ggaccacggc agataaacaa cccgtttgct ggtcagtgac ccaacgttgg caaactgtcg
     3121 cgctggggat gccacgactc tgttcagcgc agcgtttggt gggggcaatg gtcgaggaaa
     3181 tcttctctgt aaatttggcg taaataatca gttacatcaa tgagtcctaa acgaaatcca
     3241 tgcgtgaagt tgatcacaaa tttaaatact ggtagggtaa aaaggtcatt aactgcccaa
     3301 ttcaggcgtc aactggtttg attgtacatt ccttaaccgg agggtgtaag caaacccgct
     3361 acgcttgtta cagagattgc atcctgcaat tcccgctccc cttttgcggc cgtcgcgctg
     3421 atttttctgg cgtttgcgga aatgggccaa ctctgcgagg aaagctatgc tgaaaaggaa
     3481 aaaagtaaaa ccgattaccc ttcgtgatgt caccattatt aatgacagta aactgcgtaa
     3541 agccattacc gcagcatcac tgggtaatgc aatggaatgg ttcgattttg gtgtttatgg
     3601 ttttgttgct tacgcattag gtaaagtttt tttcccgggg gctgacccca gcgtgcagat
     3661 ggttgctgca cttgccactt tctccgttcc ctttctgatt cgaccgcttg gcggactctt
     3721 ctttggtatg ttgggcgata aatatggtcg ccagaagatc ctcgctatca ctattgtgat
     3781 tatgtcgatc agtacgttct gtattggctt aataccgtcc tacgacacga ttggtatttg
     3841 ggcaccgatt ctgctgttga tctgtaagat ggcacaaggt ttctcggtcg gcggtgaata
     3901 taccggggcg tcgatatttg ttgcggaata ctcccctgac cgtaaacgtg gctttatggg
     3961 cagctggctg gacttcggtt ctattgccgg gtttgtgctg ggtgcgggcg tggtggtgtt
     4021 aatttcgacc attgtcggcg aagcgaactt cctcgactgg ggctggcgta ttccgttctt
     4081 tattgctctg ccgttaggga ttatcgggct ttacctgcgc catgcgctgg aagaaactcc
     4141 ggcgttccag cagcatgttg ataaactgga acagggcgac cgcgaaggtt tgcaggatgg
     4201 cccgaaagtc tcgtttaaag agattgccac taaatactgg cgcagcctgt tgacatgtat
     4261 tggtctggta attgccacca acgtgactta ctacatgttg ctgacctata tgccgagtta
     4321 tttgtcgcat aacctgcatt actccgaaga ccacggggtg ctgattatta tcgccattat
     4381 gatcggtatg ctgtttgtcc agccggtgat gggcttgctg agtgaccgtt ttggccgtcg
     4441 tccgtttgtg ctacttggta gtgttgccct gtttgtgttg gcgatcccgg cgtttattct
     4501 gattaacagt aacgtcatcg gcctgatttt tgccgggtta ctgatgctgg cggtgatcct
     4561 taactgcttt acgggcgtta tggcttctac cttgccagcg atgttcccga cgcatatccg
     4621 ttacagcgcg ctggcggcgg catttaatat ttcggtgctg gttgccggtc tgacgccaac
     4681 actggcggcc tggctggtcg aaagctcgca gaatctgatg atgcctgcct attacctgat
     4741 ggtagtggcg gtgattggtt taatcaccgg cgtaaccatg aaagagacgg caaatcgtcc
     4801 gttgaaaggt gcgacaccgg cggcgtcaga tatacaggaa gcgaaggaaa ttctcgtcga
     4861 gcattacgat aatatcgagc agaaaatcga taatattgac cacgagattg ccgatttgca
     4921 ggcgaaacgt acccgcctgg tgcagcaaca tccgcgaatt gatgaataag ctgaaacgga
     4981 tggcctgatg tgacgctgtc ttatcaggcc aattgaactc ttaaggttca cttaatctct
     5041 gacgcgcata ctctcctcca ggttaacgga ggagagtgca atgaaaaacc gtgtttatat
     5101 gaaagtttaa ctaccgtgtt cagcgtgctg gtggtcagca gctttcttta tatctggttt
     5161 gccacgttct gatctttctt cagccgtacc taggcccgcg tgccggaagt ctcttgccgg
     5221 ttttgcagga aaaactgccc gtgatgcaac tgtgtaatgc ggctgacaat acttaacccc
     5281 agaccaatcc cgccataacg gctgtccata cgtacaaacg ctttactcaa ctccccgcat
     5341 ttactctcat caatacctgg tccttcatct tcaactgcca tgaccgctcc gccatcttct
     5401 tgcagcttaa tcataatgtt gctgccttgc gggctgtaac gatgggcgtt ttctaccagg
     5461 tttcgcaata acatccgcag cagggttgca tcaccctgaa cggtgatgtc ggcggcgctc
     5521 tctggcaata gcagggtttg ctgtcgctgg tcgagcatgg tactgagttc gtcatacgag
     5581 gggagaatga catcttccag cagttttaca tgttgataat taccggaaga aaatgactgt
     5641 ccggcacgcg ccagttgcag cagctgggag acgctctcca tcatctgatc aagccgtgcc
     5701 actaacggtg ctacatcaat gtgatgcgtt ttcgccagca gttccagatg caaacgcacc
     5761 cccgccagtg gcgttcgcag ttcgtgcgcg acgtcagcgg taaacaacct ttcgttatcc
     5821 agcgtgctgg tcaggcgact gaccagatcg ttcaacgccg aaaccaccgc ttcgatttcg
     5881 agggtggcgc tgtgaatggc aatgggcgtt aagttgtcgg cagtgcgcgc ttccagctct
     5941 ttttgcagct ccgccagcgg gcgggtgatg cggcgtaccg cctgatagca gataaatagc
     6001 gtcaggctga ccataaagac gccggggaca atcaggctgg cgaccgcctc gcggatctca
     6061 cgcatgatgt ggcgatcgtt gttgcgattg tcgcgtagcg cctgctcaaa cagctgaatc
     6121 tgctcggtac tttcatgcca tagccagaag acgctgatca gctcaaacac caacaaaatg
     6181 gccccgatag tcaatatcag ccgttggcgc agcgatattg gtcggcgcag aaaacgcatc
     6241 agattcaatt agttttcctc attcgcgacc agcatatagc caaagccgcg cacggtgcgg
     6301 atacgggatt tgcccacttt gtcgcgcaga ttgtggatat gcacttccag ggtgttggtc
     6361 gagggttcat tgtcccagtt atagatgtcg ttgtagagaa tttcccgatg taccggactg
     6421 cctgctttga gcattaaccg tgacagcaga gcatattctt tgggcgtcag aatcaactct
     6481 tcaccgccca tccatacctg acggcgaccc atattcagcg tcagattgcc aacaatcagc
     6541 tcactttcgc cctgattatt atggcgtcgt agcagggcgc ggatacgggc gtgtaactct
     6601 tccagcgcaa aaggcttcac cagatagtca tcggcaccga catccagccc ggcgattttg
     6661 tcggtcagcg tatcgcgagc ggtgaggatc agtaccggca gggtatattt tttctgccgg
     6721 atacgggcga gaaaatgcag tccatcttcg tcgggtaacc ctaaatccag taccaccagg
     6781 ctgtaatgac cggcttcaag gctttgttcc gccatccgcg cggttgtcac gccatcgcac
     6841 gcgtagcctt cggtttgcgc cgccagaatc agtccctgca ataacagcgt atcgtcttca
     6901 acaatcagaa ttttcattta ctcactctcc tgcaagtttg cagaatatca tccgcagcct
     6961 ggtaatactt cgtctcaacg ccagttaatc ccaatagcgt ggagaataaa ttgtcttgtg
     7021 aatagtgttg cgtttgcgcc tgtttttgca ggcagttctg gtcaacctga taccgttttt
     7081 gataatcctc cgacagccac agcagcatcg gcacctgttt ttggctatcc ggggcgatgg
     7141 cataaggcag accgtgcaga tagatgccat tttcacctaa cgattcaccg tggtcagaaa
     7201 gataaaccag gctggtggta aatttatcct gatgttcttt cagcagatta atcgctttat
     7261 caacaatata gtcgacgtaa accagcgtgt tgtcgtaagt gttcaccagt tgctctttgg
     7321 tacaggtctg gatctcattg gtgtcgcagg ttggggtaaa tttcctgaac tgcggcggat
     7381 agcggttgta ataggtcgga ccgtggctgc cgatggtgtg taagacaatc acgccatcac
     7441 cctgcaggtt attgatgtac tcttccagcc cgtggaacag cacttcgtca tagcattcgc
     7501 cgttgatgca ctgaccaggc agattcagcg cggtgacgtt ctggtgaggc acgcggtcgc
     7561 aggcaccttt acagccgcca tcgttgtcat tccacagcac gttgatgccc gctcgctgaa
     7621 tgatatccag cacgccttcc tggtgctgtg ccagctcttc tttgtaatgc tcacgcggca
     7681 tatccgagaa catgcacggt actgaaactg ccgttgccgt gccgcaagat acggtattag
     7741 ggaaatagac cacgttatct ttcgccagcc gcgggttagt ttcacgcggg tagccgttga
     7801 gggagaagtt ctccgcccgc gaggtttcgc cgacaatcag gatggtcaaa tttttacgtt
     7861 tttcgttctg cattaacggg ttgcggtggg cgtcttcacc aattcgcacc agcggtagat
     7921 ttgccagtcg ctgatgggag taccatgacc agctggcaac aatgctgtta gaggggctta
     7981 aggatttcac cagctctttg ttattgcgga acaacgaggc gtagtcttta taaaacagtg
     8041 cggcgaccag caaaatcagt agtacagaaa ccagaatatt ggctccacgg aaaagaacac
     8101 tgcgcagacg cgaggtggca ggtttgattt ttatccagca ggcaatcagc gcagcaagca
     8161 cgccgctgaa tcccagcgtt aataacattt gcggtgtcat cagcgcataa ctttctgccg
     8221 gagtggtatc aataatattg gcaatcatcg agcggtcgat gacgatgccg taagtcatta
     8281 tgaaatattg tgcagccgcg ccaaccagaa taaacaggca ggccagtggt cgattaagcc
     8341 ataagaaaga gcttagtgtc aggacaatat taatcacgct gaaagcgacg accggcatcg
     8401 acaagaaaac cagtacgtta tgcagcgaat ccagcggcag cgcctgcaac acctgtttaa
     8461 aaaaggcaat attcaggcag atagagatat aaaaagcggc caacaatagc caggcgagta
     8521 aattcaaaga gggtcttttt agtaggcgct tcaacatcac ggtgtttcca tcgaacaaag
     8581 tgcgcatatg ctcgcaaagc aaaattaagc caaccttaag ttcttaaggt tggcttttat
     8641 gtttgctgga ttaaggggaa ttaatctttg cttattggtg catctaaggg atacgggttt
     8701 ttatgtagcc ggttgtaatt cagcgcatac atggcggtga tgaccatcag ggtgacaaat
     8761 gaccacataa cctctttcgc tccggacccc accacggccc agatgcagta gaggaaggca
     8821 atggtagtaa ctgccagata tgccgggcgt gctttaccaa agtgaccgtg tccgagcagc
     8881 agtaacgccg cacaggtgta aagatatggc accagtgtaa agatgaccga gacggaagaa
     8941 accagaccga actctttggt cgcgtttggt gaaatgctgc tgagctggaa gatggtcatc
     9001 aaaataccga cgataatcag ccccgccact ggcgtacccg ctttatttac acgggcaaaa
     9061 atcggtggga acagtccgtc atcggcagcg gctttcgccg tttgacccgc cagcaacgtc
     9121 cagccgccca gtgaacctaa gcaacccgca gctgcgcaga aggaaacaat ggccccgggg
     9181 gtgtcaccca acgccatccg tgcggcatca ccgaatggcg aagcagaaac gcgcagtgcg
     9241 gcattaggga tcatccccat aatcgcggtg gtagaaagta catagcaaac ggcggcaatc
     9301 aataccccac caatggtggc gatagggaca ttgcgtttcg ggtttttcac cacacctgcg
     9361 gcaacggagg cactttccac accgatgaac gaccacagcg taacgttaag ggtactttga
     9421 attgcaccga aggtacccag gccgctgacg ttccatgccg ccatataggt ttcaccacgg
     9481 aaccagaacc agccaaatac ggcaatcccg acgatgggga tcagcgccag cacggtggca
     9541 actgcctgca cacgggtgat cattttcgga ccaacaatgt tcagcaggac gaagatccac
     9601 agcaccacga cgcaggtgat ggttaatacc aatggatctt tcagaatcgg gaagaagtaa
     9661 cttaaatatc ctacgccaat gaccaccatg gcgatattgc cgatccagca ggccagccag
     9721 tagaggacgt tggtttgata accgagaaac gggccaaagc agcggcgggc gtaagcgtaa
     9781 gaaccaccag gacttgggtc gaggaacgac attttggcgt ataccatcga gagccccagt
     9841 gcaccgataa tcgtcaccaa ccatccataa atggcaatcc cgccagtgga ggccaggttt
     9901 gcaggtaaca gaaaaacacc tgaccccata atattccccg acaccatcag ggtgacgggg
     9961 attaagccca ctttgtgagc atcagcatcc gaagacataa ttaaactcct gcgaaggcga
    10021 gcttcgtgac aataaattac gtcatatcat acgcctgcat tcgatagttg aattattatc
    10081 gaccgggtta tcgctgatgc cgttattaat atagctcttc gttaaggtta aaaatcaggc
    10141 gacagtgcgt tctctgtgct gactaacata atgcagcggc gtcataccgt agaagtcttt
    10201 aaagacagaa ataaagtacg acgtactgtt gtagccgcag gactgtgata cctgagagat
    10261 atttttaccg tccatcatta attcatttac ggcataacgc atgcggcagg tggtgattat
    10321 ttggctataa ctggtgtttt cgcttttcag ctttttcttt aacagacttg ggctaagaca
    10381 taaacaactg gctaccatac taagattcca gtctttgtga atatcgcttt caataatttg
    10441 ataaacgctg tcacttacac aattacgtaa catatacatc attaatgaaa ggaatttttt
    10501 actgtcgaga aaacgggata gcaccgtgaa taataatgcg cgtgttcgtt ccttttctga
    10561 ctcagtttct gcgggcatca tgctgtgttg cgcggcaacg cgaaagacgt ctggcgtcag
    10621 gcatggcagg gtgagtatgg gcgtagcgct acgttgccat actggtattt gcgagagatc
    10681 tttattcaga aaccggaggt aatctttgat gatgtcgtga ctaatatgag caaccaaagt
    10741 gttgttaaga gaggagacgt cgataatatt attttcacaa tttaataacg ccatatggtt
    10801 agctttaagg ctaataggtt ctttcccatt cacccttatc cagacatctt tttcagtcaa
    10861 taaaacaata caaggttggt cgctgcaaat cctcatacat gtactcctga gtgcgaataa
    10921 aagaccagtt ctcgctaaag caaagcgata ccgggaaatg gttatataat tgtatgacga
    10981 atataaacat gcataaaaat aacgagtttt actaatatta caaattttta tagtaatttc
    11041 tcatgtacat atttttttac agggtaaata acaggcaaaa aaaagcgcgg ggtggggccg
    11101 cgcaaaaagt atgacatttt tgtttgcagc aatttttatg aaaacgttgc catactcaaa
    11161 aggcctaagg ccaggcggcg taatgttatt taaacaatta cgccttcagc ggaatagtgg
    11221 ttacgctttc acacacatag cgtggtaaat accgtcaata atttcagtcc cttcagtttc
    11281 gtgttcaaat ccagggaaat ggtggtccca ggattgcagc gagcgtaaat aacttacttg
    11341 cggactgttt ttatcgccga agttttcacc agacagcagc atcgggattc ctggcggata
    11401 tgggataact gagtttgccg cgatgcgtcc tggcagattt tcaatggata ccagttcgac
    11461 attgttgtcg acaatcgcgt tgtacgcttc acgcggggtg atttccgcca ccggcaggcc
    11521 ggaataggct tcgttcaacc gtgcgccagg gttgttttct ttcagccagg caaacatggt
    11581 gtcacccaga tcgtgaatcc ccatgttcgc gtaagtgtca ggatattgtt caacaagttc
    11641 cggcatcacc tgcgccagcg gtgtgttggc gtcatagtgg cgtttgaagg agcaaagggt
    11701 gttaaccaga gtcccccatt tcccacgggt tacgcccata gagaacagga acataatttg
    11761 gaagtcagtg gtgcgggtag gcacaatgcc gtggcgacca agccaggcag tgaccagcgc
    11821 cgccggaaca ccggtttctt ccagttcacc atcttcaccc attcccggag caaggatgct
    11881 gactttaatc gggtcgagca tactccagtt atccggaata tctttgaagc cgtgccagct
    11941 ttcgcccgga tgcattaccc agtagtcctg aacggtggtc agcagtttgg ttggtgcgtc
    12001 agcaaagtca taggttttgc cggtttgtgg gtcggtgacg acttctttgt tccacggttt
    12061 gaagaaccag ctaccgtcag cggtgaactc tttatacagc cgcgccatcg cctgacggaa
    12121 atcaaccgct tcgtcgatca cttcctgcgt cagtgacagg ccgctgttgc cgtccatcat
    12181 cgacaccgcc acgtcgttgg atgcgcagat ggcatacagc ggggaggtgg tggcatgcat
    12241 catgtaggcc tggttgaagc gggagaagtt aatcgcccca cgaccttcac gtacatgaat
    12301 ataagaagcc tgtgaaagcg cattcagcag tttgtgggtg gagtgggtgg cgaaaacggt
    12361 aggaccgttg tgatcgccag gttcgccgcg catggcatag tgatcggcat agatcgggtt
    12421 gaaacgtgca tagccgtacc aggcttcgtc aaagtgcaga cgatcggagg ttttttccag
    12481 cagatcctgc gcttctttag cgttataaca cacgccgtca taggtgcagt tggtcaccac
    12541 gcagtaagac ggtttttgcc cggctttgtc tttagtcagc gggctttcac tgattttctt
    12601 ctgcaaggtt tcaggttgca tttcctgcgg atagattggc ccgataatgc cgtagcggtt
    12661 gcggcttggc accatgtaga ccggtttcgc gcctgtcagc atcaagcctt gttcgatgga
    12721 tttatggcag ttacggtcaa cgaccacgac atcgttatcg gtcatgcaag cctgcatgat
    12781 ggtgcggtta gagccggaag taccgacgac taccgaccag gagcgatcgg caccaaatac
    12841 gcgtgcggca tatttttcgc tttcgccaaa tgcgccagta tggtcaagca aagaaccgag
    12901 ggaagttcgt tcgatgccca tatcggtgcg gaacagattt tctccatagt agtcatggta
    12961 gaaacgtccg gcgggtgttt tggtaaaacc aacgccgccc tggtggcctg gcgctgccca
    13021 ggaatattca tggatgtcgc tatacttcat cagcgcgctg aacagtggcg gcaacagctg
    13081 ctggcggtag cgggtcatcg cggcaacggc gcgtccggcg ataaagtcgg tggtatcttc
    13141 cagaatccag gcgaattcat cgacaagctc cagcaggtcg cgatccattg cggcgagggc
    13201 tttttcccga tcgcccaaca ggaagaccgg cacgttttgt tggcgctcat gaagcttacc
    13261 gatcaattgt ctgacgtttt gatgttcgtc cggatgttcc atttgatagc tgaacatcag
    13321 gcagtcaatg gcttcgtttg aagagagaat ggcaaaacca tcatcaaagg aggtggattt
    13381 aatcacggta acattttgct ggcttaaagc atctgccaga cgctcaacgg cgttaccgac
    13441 ccaggtgtct tgatggagaa actcgctttc aacaattaat actttcatca ttgcttaccc
    13501 ggttatgaag gaatcttcgt tgcgggcaag tatcttccgg ccttttcggg ctgtaaagcg
    13561 cgtgaaaaac aaacattttt gaaatattta tttttataaa caatgcgtta tgtgattatt
    13621 ttaaattatt ttcactgctt gttatcgacg gctaaactat ttttttggct gatactgata
    13681 tcgtctttag ccgggaaacg tctggcggcg ctgttggcta agtttgcggt attgttgcgg
    13741 cgacatgccg acatatttgc cgaacgtgct gtaaaaacga ctacttgaac gaaagcctgc
    13801 cgtcagggca atatcgagaa tacttttatc ggtatcgctc agtaacgcgc gaacgtggtt
    13861 gatgcgcatc gcggtaatgt actgtttcat cgtcaattgc atgacccgct ggaatatccc
    13921 cattgcatag ttggcgttaa gtttgacgtg ctcagccaca tcgttgatgg tcagcgcctg
    13981 atcatagttt tcggcaataa agcccagcat ctggctaaca taaaattgcg catggcgcga
    14041 gacgctgttt ttgtgtgtgc gcgaggtttt attgaccaga atcggttccc agccagagag
    14101 gctaaatcgc ttgagcatca ggccaatttc atcaatggcg agctggcgaa tttgctcgtt
    14161 cggactgttt aattcctgct gccagcggcg cacttcaaac gggctaagtt gctgtgtggc
    14221 cagtgatttg atcatcatgc cgtgagtgac gtggttaatc aggtctttat ccagcggcca
    14281 ggagagaaac agatgcatcg gcagattaaa aatcgccatg ctctgacagg ttccggtatc
    14341 tgttagttgg tgcggtgtac aggcccagaa cagcgtgata tgaccctgat tgatattcac
    14401 tttttcattg ttaatcaggt attccacatc gccatcgaaa ggcacattca cttcgacctg
    14461 accatgccag tggctggtgg gcatgatatg cggtgcgcga aactcaatct ccatccgctg
    14521 gtattccgaa tacagcgaca gcgggctgcg ggtctgtttt tcgtcgctgc tgcacataaa
    14581 cgtatctgta ttcatggatg gctctctttc ctggaatatc agaattatgg caggagtgag
    14641 ggaggatgac tgcgagtggg agcacggttt tcaccctctt cccagagagg cgaggggagc
    14701 gtccgagcat catgaggccg aaaactctgc ttttcaggta acttattacc ataaactcag
    14761 atttactgct gcttcacgca ggatctgagt ttatgggaat gctcaacctg gaagccggag
    14821 gttttctgca gattcgcctg ccatgatgaa gttattcaag caagccagga gatctgcatg
    14881 atgactgcac ccaaaattac atttatcggc gctggttcga cgattttcgt taaaaatatt
    14941 cttggtgatg tgttccatcg agaggcgctg aaaacggcgc atattgccct gatggacatt
    15001 gatcccaccc gcctggaaga gtcgcatatt gtggtgcgta agctgatgga ttcagcaggg
    15061 gccagcggca aaatcacctg ccacacccaa cagaaagaag ccttacagga tgccgatttt
    15121 gtcgtggtgg catttcagat tggcggttat gaaccttgca cggtgactga tttcgaggtc
    15181 tgtaagcggc atggtctgga acaaaccatt gccgatacgt tggggccggg cggtattatg
    15241 cgcgcgctac gtaccattcc gcatctgtgg caaatttgcg aggacatgac ggaagtctgc
    15301 cccgatgcca cattgctcaa ctacgttaac ccaatggcga tgaatacctg ggcgatgtat
    15361 gcccgctatc cgcatatcaa acaggtaggg ctgtgccatt cggtgcaggg aacggcggaa
    15421 gagctggcgc gtgacctcaa tatcgatccg tccacgctgc gttaccgttg cgctggtatc
    15481 aaccatatgg cgttttacct ggaactggag cgtaaaaccg ccgacggcag ttacgtgaat
    15541 ctctacccgg aactgctggc ggcttatgaa gcagggcagg caccgaagcc acatattcat
    15601 ggtaatactc gctgccagaa tattgtgcgc tacgaaatgt tcaaaaagct gggctacttc
    15661 gtcacggaat cgtcagaaca ttttgccgaa tatacgccgt ggtttattaa gccaggtcgt
    15721 gaggatttga ttgagcgtta taaagtaccg ctggatgagt acccgaaacg ctgcgtcgag
    15781 cagctggcga actggcataa agagctggag gagtataaaa acgcctcccg gattgatatt
    15841 aaaccgtcac gggaatatgc cagcacaatc atgaacgcta tctggactgg cgagtcgagt
    15901 gtgatttacg gcaacgtccg taacgatggt ttgattgata acctgccaca aggatgttgc
    15961 gtggaagtag cctgtctggt tgatgctaat ggcattcagc cgaccaaagt cggtacgcta
    16021 ccttcgcatc tggccgccct gatgcaaacc aacatcaacg tacagacgct gctgaccgaa
    16081 gctattctta cggaaaatcg cgaccgtgtt taccacgccg cgatgatgga cccgcatact
    16141 gccgccgtgc tgggcattga cgaaatatat gctcttgttg acgacctgat tgccgcccac
    16201 ggcgactggc tgccaggctg gttgcaccgt taaaacgcga ctaaacgcta ctgcgccggg
    16261 ggatttattc cggcgcacac ctctgacgat accaataaca gaaggcgggc gttggtaaca
    16321 gcgacccgat accctatgag catttcaatg actacaaaac tcagttatgg atttggagcg
    16381 ttcgggaagg attttgcgat cggcattgtg tatatgtacc tcatgtatta ctacaccgat
    16441 gtcgttgggc tgtctgtggg tttggtcggt actttgtttc tggtggcgag gatctgggat
    16501 gctattaacg atccgattat g
//
LOCUS       DAJVUJ010000094        16472 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.094, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000094 DAJVUJ010000000
VERSION     DAJVUJ010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16472
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.094"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(118..1401)
                     /gene="gltA"
                     /locus_tag="OQQ48_002656"
     CDS             complement(118..1401)
                     /gene="gltA"
                     /locus_tag="OQQ48_002656"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HCS1953371.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            complement(1607..1714)
                     /locus_tag="OQQ48_002657"
     CDS             complement(1607..1714)
                     /locus_tag="OQQ48_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1953372.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            2095..2499
                     /gene="sdhC"
                     /locus_tag="OQQ48_002658"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             2095..2499
                     /gene="sdhC"
                     /locus_tag="OQQ48_002658"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCS1953373.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            2493..2840
                     /gene="sdhD"
                     /locus_tag="OQQ48_002659"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             2493..2840
                     /gene="sdhD"
                     /locus_tag="OQQ48_002659"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HCS1953374.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            2840..4606
                     /gene="sdhA"
                     /locus_tag="OQQ48_002660"
     CDS             2840..4606
                     /gene="sdhA"
                     /locus_tag="OQQ48_002660"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HCS1953375.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            4622..5338
                     /gene="sdhB"
                     /locus_tag="OQQ48_002661"
     CDS             4622..5338
                     /gene="sdhB"
                     /locus_tag="OQQ48_002661"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HCS1953376.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            5639..8440
                     /gene="sucA"
                     /locus_tag="OQQ48_002662"
     CDS             5639..8440
                     /gene="sucA"
                     /locus_tag="OQQ48_002662"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HCS1953377.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYSDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            8455..9672
                     /gene="odhB"
                     /locus_tag="OQQ48_002663"
     CDS             8455..9672
                     /gene="odhB"
                     /locus_tag="OQQ48_002663"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HCS1953378.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKSPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLITIK
                     ELLEDPTRLLLDV"
     gene            9948..11114
                     /gene="sucC"
                     /locus_tag="OQQ48_002664"
     CDS             9948..11114
                     /gene="sucC"
                     /locus_tag="OQQ48_002664"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HCS1953379.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPQLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQLDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKVVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            11114..11983
                     /gene="sucD"
                     /locus_tag="OQQ48_002665"
     CDS             11114..11983
                     /gene="sucD"
                     /locus_tag="OQQ48_002665"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HCS1953380.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(12087..12809)
                     /gene="mngR"
                     /locus_tag="OQQ48_002666"
     CDS             complement(12087..12809)
                     /gene="mngR"
                     /locus_tag="OQQ48_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HCS1953381.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            12978..14894
                     /gene="mngA"
                     /locus_tag="OQQ48_002667"
     CDS             12978..14894
                     /gene="mngA"
                     /locus_tag="OQQ48_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HCS1953382.1"
                     /translation="MNLTTLTHRDALRLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LEEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTMHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVPACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALTLKRSDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAIFGFMCSFDLGGPVNKAAYAFCLGAMVNGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLVGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            14912..>16472
                     /locus_tag="OQQ48_002668"
     CDS             14912..>16472
                     /locus_tag="OQQ48_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HCS1953383.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPTDENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMDKLREIYPQRKFVMSRFEEVFEKIEAQRESLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKIAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGSLQLV
                     DKDSGVRYDRVL"
BASE COUNT         3955 a         4134 c         4485 g         3898 t
ORIGIN      
        1 aatatatgaa tgtccgatag tggattttat ttttacaatt ttttgtgttt aggcatataa
       61 aaattaaccc gccatttgaa cggcgggtta aaatatttac aacttagcaa tcaaccatta
      121 acgcttgata tcgcttttaa agtcgcgttt ttcatatcct gtatacagct gacgcggacg
      181 ggcaatcttc ataccgtcac tgtgcatttc gctccagtgg gcgatccagc caacggtacg
      241 tgccattgcg aaaatgacgg tgaacatgga agacggaata cccatcgctt tcaggatgat
      301 accagagtag aaatcgacgt tcgggtacag tttcttctcg ataaagtacg ggtcgttcag
      361 cgcgatgttt tccagctcca tagccacttc cagcaggtca tctttggtgc ccagctcttt
      421 cagaacttca tggcaggttt cacgcattac ggtggcgcgc gggtcgtaat ttttgtacac
      481 gcggtgaccg aagcccatca ggcggaaaga atcattttta tctttcgcac gacgaacaaa
      541 ttccggaatg tgtttaacgg agctaatttc ttccagcatt ttcagcgccg cttcgttagc
      601 accaccgtgc gcaggtcccc acagtgaagc aatacctgct gcgatacagg caaacgggtt
      661 cgcacccgaa gagccagcgg tacgcacggt ggaggtagag gcgttctgtt catggtcagc
      721 gtgcaggatc agaatacggt ccatagcacg ttccagaatc ggattaactt catacggttc
      781 gcacggcgtg gagaacatca tattcaggaa gttaccggca taggagagat cgttgcgcgg
      841 ataaacaaat ggctgaccaa tggaatactt gtaacacatc gcggccatgg tcggcatttt
      901 cgacagcagg cggaacgcgg caatttcacg atgacgagga ttgttaacat ccagcgagtc
      961 gtgatagaac gccgccagcg cgccggtaat accacacatg actgccattg gatgtgagtc
     1021 gcgacggaaa gcgtggaaca gacgggtaat ctgctcgtgg atcatggtat gacgggtcac
     1081 cgtagtttta aattcgtcat actgttcctg agtcggtttt tcaccattca gcaggatgta
     1141 acaaacttcc aggtagttag aatcggtcgc cagctgatcg atcgggaaac cgcggtgcag
     1201 caaaatacct tcatcaccat caataaaagt aattttagat tcgcaggatg cggttgaagt
     1261 gaagcctggg tcaaaggtga acacaccttt tgaaccgaga gtacggatat caataacatc
     1321 ttgacccagc gtgcctttca gcacatccag ttcaacagct gtgtccccgt tgagggtgag
     1381 ttttgctttt gtatcagcca tttaaggtct ccttagcgcc ttattgcgta agactgccgg
     1441 aacttaaatt tgccttcgca catcaacctg gctttacccg tttgttattt ggctcgccgc
     1501 tctgtgaaag aggggaaaac ctgggtacag agctctgggc gcttgcaggt aaaggatcca
     1561 ttgatgacga ataaatggcg aatcaagtac ttagcaatcc gaattattaa acttgtctac
     1621 cactaataac tgtcccgaat gaattggtca atacttccac actgttacat aagttaatct
     1681 taggtgaaat accgacttca taacttttac gcattatatg cttttcctag taatgtttgt
     1741 aacaactttg ttgagtgatt gtcaaattag atgattaaaa attaaataaa tgttgttgtc
     1801 gtgacctgga tcactgttca ggataaaacc cgacaaacta tatgtaggtt aattgtaatg
     1861 attttgtgaa cagcctatac tgccgccagg tctccggaac accctgcaat cccgagccac
     1921 ccagcgttgt aacgtgtcgt tttcgcatct ggaagcagtg ttttgcatga cgcgcagtta
     1981 tagaaaggac gctgtctgac ccgcaagcag accggaggaa ggaaatcccg acgtctccag
     2041 gtaacagaaa gttaacctct gtgcccgtag tccccaggga ataataagaa cagcatgtgg
     2101 gcgttattca tgataagaaa tgtgaaaaaa caaagacctg ttaatctgga cctacagacc
     2161 atccggttcc ccatcacggc gatagcgtcc attctccatc gcgtttccgg tgtgatcacc
     2221 tttgttgcag tgggcatcct gctgtggctt ctgggtacca gcctctcttc ccctgaaggt
     2281 ttcgagcaag cttccgcgat tatgggcagc ttcttcgtca aatttatcat gtggggcatc
     2341 cttaccgctc tggcgtatca cgtcgtcgta ggtattcgcc acatgatgat ggattttggc
     2401 tatctggaag aaacattcga agcgggtaaa cgctccgcca aaatctcctt tgttattact
     2461 gtcgtgcttt cacttctcgc aggagtcctc gtatggtaag caacgcctcc gcattaggac
     2521 gcaatggcgt acatgatttc atcctcgttc gcgctaccgc tatcgtcctg acgctctaca
     2581 tcatttatat ggtcggtttt ttcgccacca gtggcgaact gacatatgaa gtctggatcg
     2641 gtttcttcgc ctctgcgttc accaaagtgt tcaccctgct ggcgctgttt tctatcttga
     2701 ttcatgcctg gatcggcatg tggcaggtgt tgaccgacta cgttaaaccg ctggcattgc
     2761 gcctgatgct gcaactggtg attgtcgttg cactggtggt ttacgtgatt tatggattcg
     2821 ttgtggtgtg gggtgtgtga tgaaattgcc agtcagagaa tttgatgcag ttgtgattgg
     2881 tgccggtggc gcaggtatgc gcgcggcgct gcaaatttcc cagagcggcc agacctgtgc
     2941 gctgctctct aaagtcttcc cgacccgttc ccataccgtt tctgcgcaag gcggcattac
     3001 cgttgcgctg ggtaataccc atgaagataa ctgggaatgg catatgtacg acaccgtaaa
     3061 agggtcggac tatatcggtg accaggacgc gattgaatat atgtgtaaaa ccgggccgga
     3121 agcgattctg gaactcgaac acatgggcct gccgttctcg cgtcttgatg atggtcgtat
     3181 ctatcaacgt ccgtttggcg gtcagtcgaa aaacttcggc ggcgagcagg cggcacgcac
     3241 tgcggcggcg gctgaccgta ccggtcacgc actgttgcac acgctttatc agcagaacct
     3301 gaaaaaccac accaccattt tctccgagtg gtatgcgctg gatctggtga agaaccagga
     3361 tggcgcggtg gtgggttgta ctgcactgtg catcgaaact ggtgaagtgg tttatttcaa
     3421 agcccgcgct accgtgctgg cgactggcgg ggcagggcgt atttatcagt ccaccactaa
     3481 cgcccacatt aacactggcg acggcgtcgg catggctatc cgcgcaggcg taccggtgca
     3541 ggatatggaa atgtggcagt tccacccgac cggtattgcc ggtgcgggcg tactggtcac
     3601 cgaaggttgc cgtggtgaag gcggttatct gctgaacaaa catggcgaac gctttatgga
     3661 gcgttatgcg ccgaacgcca aagacctggc gggccgtgat gtggtggcgc gttccatcat
     3721 gatcgaaatc cgtgaaggtc gcggctgtga tggtccgtgg gggccacacg cgaaactgaa
     3781 actcgaccac ctgggtaaag aagttctcga atcccgtctg ccgggcatcc tggaactctc
     3841 ccgtaccttc gctcacgtcg atccggtgaa agagccgatc ccggttatcc caacctgtca
     3901 ttacatgatg ggcggtattc cgaccaaagt gaccggtcag gcgctgactg tgaatgagaa
     3961 aggcgaagat gtggttgttc cgggactgtt tgccgttggt gaaatcgctt gtgtatcggt
     4021 acacggcgct aaccgtctgg gcggcaactc gctgctggac ctggtggtct ttggtcgtgc
     4081 ggcaggtctg catctgcaag agtctatcgc cgagcagggc gcattgcgcg atgccagcga
     4141 gtctgatgtt gaagcatctc tggatcgtct gaaccgctgg aacaataatc gtaacggtga
     4201 agatccggtg gcgattcgta aagcgctgca agaatgtatg cagcataact tctcggtctt
     4261 ccgtgaaggt gatgcgatgg cgaaagggct tgagcagttg aaagttatcc gcgagcgtct
     4321 gaaaaatgcc cgtctggatg acacttccag cgagttcaac acccagcgcg ttgagtgcct
     4381 ggaactggat aacctgatgg aaacggcgta tgcaacggct gtttctgcca acttccgtac
     4441 cgaaagccgt ggcgcgcata gccgcttcga cttcccggat cgtgatgatg aaaactggct
     4501 gtgccactcc ctgtatctgc cagagtcgga atccatgacg cgccgaagcg tcaacatgga
     4561 accgaaactg cgcccggcat tcccgccgaa gattcgtact tactaatgcg gagacaggaa
     4621 aatgagactc gagttttcaa tttatcgcta taacccggat gttgatgatg ctccgcgtat
     4681 gcaggattac accctggaag cggaagaagg tcgcgacatg atgctgctgg atgcgcttat
     4741 ccagctgaaa gagaaagatc ccagcctgtc gttccgccgc tcctgtcgtg aaggtgtgtg
     4801 cggttccgac ggtctgaaca tgaacggcaa gaatggtctg gcctgtatta ccccgatttc
     4861 ggcactcaac cagccgggca agaagattgt gattcgcccg ctgccaggtt taccggtgat
     4921 ccgcgatttg gtggtagaca tgggacaatt ctatgcgcaa tatgagaaaa ttaagcctta
     4981 cctgttgaat aatggacaaa atccgccagc tcgcgagcat ttacagatgc cagagcagcg
     5041 cgaaaaactc gacgggctgt atgaatgtat tctctgcgca tgttgttcaa cctcttgtcc
     5101 gtctttctgg tggaatcccg ataagtttat cggcccggca ggcttgttag cggcatatcg
     5161 tttcctgatc gatagccgtg ataccgagac tgacagccgc ctcgacggtt tgagcgatgc
     5221 attcagcgta ttccgctgtc acagcatcat gaactgcgtc agtgtatgtc cgaaggggct
     5281 gaacccgacg cgcgccatcg gccatatcaa gtcgatgttg ttgcaacgta atgcgtaaac
     5341 cgtaggcctg ataagacgcg caagcgtcgc atcaggcaac cagtgccgga tgcggcgtga
     5401 acgccttatc cggcctacaa gtcattaccc gtaggcctga taagcgcagc gcatcaggcg
     5461 taacaaagaa atgcaggaaa tctttaaaaa ctgcccctga cactaagaca gtttttaaag
     5521 gttccttcgc gagccactac gtagacaaga gctcgcaagt gaaccccggc acgcacatca
     5581 ctgtgcgtgg tagtatccac ggcgaagtaa gcataaaaaa gatgcttaag ggatcacgat
     5641 gcagaacagc gctttgaaag cctggttgga ctcttcttac ctctctggcg caaaccagag
     5701 ctggatagaa cagctctatg aagacttctt aaccgatcct gactcggttg acgctaactg
     5761 gcgttcgacg ttccagcagt tacctggtac gggagtcaaa ccggatcaat tccactctca
     5821 aacgcgtgaa tatttccgcc gcctggcgaa agacgcttca cgttactctt caacgatctc
     5881 cgaccctgac accaatgtga agcaggttaa agtcctgcag ctcattaacg cataccgctt
     5941 ccgtggtcac cagcatgcga atctcgatcc gctgggactg tggcagcaag ataaagtggc
     6001 cgatctggat ccgtctttcc acgatctgac cgaagcagac ttccaggaaa ccttcaacgt
     6061 cggttcattt gccagcggca aagaaaccat gaaactcggc gagctgctgg aagccctcaa
     6121 gcaaacctac tgcggcccga ttggtgccga gtatatgcac atcaccagca ccgaagaaaa
     6181 acgctggatc caacagcgta tcgagtctgg tcgcgcgact ttcaatagcg aagagaaaaa
     6241 acgcttctta agcgaactga ccgccgctga aggccttgaa cgttacctcg gcgcaaaatt
     6301 ccctggcgca aaacgcttct cgctggaagg cggtgacgcg ttaatcccga tgcttaaaga
     6361 gatgatccgc cacgctggca acagcggcac ccgcgaagtg gttctcggga tggcgcaccg
     6421 tggtcgtctg aacgtgctgg tgaacgtgct gggtaaaaaa ccgcaagact tgttcgacga
     6481 gttcgccggt aaacataaag aacacctcgg cacgggtgac gtgaaatacc acatgggctt
     6541 ctcgtctgac ttccagaccg atggcggcct ggtgcacctg gcgctggcgt ttaacccgtc
     6601 tcaccttgag attgtaagcc cggtagttat cggttctgtt cgtgcccgtc tggacagact
     6661 tgatgagccg agcagcaaca aagtgctgcc aatcaccatc cacggtgacg ccgcagtgac
     6721 cgggcagggc gtggttcagg aaaccctgaa catgtcgaaa gcgcgtggtt atgaagttgg
     6781 cggtacggta cgtatcgtta tcaacaacca ggttggtttc accacctcta atccgctgga
     6841 tgcccgttct acgccgtact gtactgatat cggtaagatg gttcaggccc cgattttcca
     6901 cgttaacgcg gacgatccgg aagccgttgc ctttgtgacc cgtctggcgc tcgatttccg
     6961 taacaccttt aaacgtgatg tcttcatcga cctggtgtgc taccgccgtc acggccacaa
     7021 cgaagccgac gagccgagcg caacccagcc gctgatgtat cagaaaatca aaaaacatcc
     7081 gacaccgcgc aaaatctacg ctgacaagct ggagcaggaa aaagtggcga cgctggaaga
     7141 tgccaccgag atggttaacc tgtaccgcga tgcgctggat gctggcgatt gcgtagtggc
     7201 agagtggcgt ccgatgaaca tgcactcttt cacctggtcg ccgtacctca accacgaatg
     7261 ggacgaagag tacccgaaca aagttgagat gaagcgcctg caggagctgg cgaaacgcat
     7321 cagcacggtg ccggaagcag ttgaaatgca gtctcgcgtt gccaagattt atagcgatcg
     7381 ccaggcgatg gctgccggtg agaaactgtt cgactggggc ggtgcggaaa acctcgctta
     7441 cgccacgctg gttgacgaag gcattccggt tcgcctgtcg ggtgaagact ccggtcgcgg
     7501 taccttcttc caccgccacg cggtgatcca caaccagtct aacggttcca cttacacgcc
     7561 gctgcaacac atccataacg ggcagggcgc gttccgcgtc tgggactccg tactgtctga
     7621 agaagcagtg ctggcgtttg aatacggtta tgccaccgca gaaccacgca ctctgaccat
     7681 ctgggaagcg cagttcggtg acttcgctaa cggtgctcag gtggttatcg accagttcat
     7741 ctcctctggc gaacagaaat ggggccggat gtgtggtctg gtgatgttgc tgccgcacgg
     7801 ttatgaaggg caggggccgg agcactcctc cgcgcgtctg gaacgttatc tgcaactttg
     7861 tgctgagcaa aacatgcagg tgtgcgtacc gtctaccccg gcacaggttt accacatgct
     7921 gcgtcgtcag gcgctgcgcg ggatgcgtcg tccgctggtc gtgatgtcgc cgaaatccct
     7981 gctgcgtcat ccgctggcgg tttccagcct cgaagaactg gcgaacggca ccttcctgcc
     8041 agccatcggt gaaatcgacg agcttgatcc gaagggcgtg aagcgcgtag tgatgtgttc
     8101 tggtaaggtt tattacgacc tgctggaaca gcgtcgtaag aacaatcaac acgatgtcgc
     8161 cattgtgcgt atcgagcaac tctacccgtt cccgcataaa gcgatgcagg aagtgttgca
     8221 gcagtttgct cacgtcaagg attttgtctg gtgccaggaa gagccgctca accagggcgc
     8281 atggtactgc agccagcatc atttccgtga agtgattccg tttggggctt ctctgcgtta
     8341 tgcaggccgc ccggcctccg cctctccggc ggtagggtat atgtccgttc accagaaaca
     8401 gcaacaagat ctggttaatg acgcgctgaa cgtcgaataa ataaaggata cacaatgagt
     8461 agcgtagata ttctggtccc tgacctgcct gaatccgtag ccgatgccac cgtcgcaacc
     8521 tggcataaaa aacccggcga cgcagtcgta cgtgatgaag tgctggtaga aatcgaaact
     8581 gacaaagtgg tactggaagt accggcatca gcagacggca ttctggatgc ggttctggaa
     8641 gatgaaggta caaccgtaac gtctcgtcag atccttggtc gcctgcgtga aggcaacagc
     8701 gccggtaaag aaaccagcgc caaatctgaa gagaaagcgt ccactccggc gcaacgccag
     8761 caggcgtctc tggaagagca aaacaacgat gcgttaagcc cggcgatccg tcgcttgctg
     8821 gctgaacata acctcgacgc cagcgctatt aaaggcaccg gcgtgggtgg tcgtctgacc
     8881 cgtgaagatg tggaaaaaca tctggcgaaa tccccggcga aagagtctgc accggcagcg
     8941 gctgctccgg cggcgcaacc ggctctggct gcacgtagtg aaaaacgtgt cccgatgact
     9001 cgcctgcgta agcgtgtggc agagcgtctg ctggaagcga aaaactccac cgccatgctg
     9061 accacgttca acgaagtcaa catgaagccg attatggatc tgcgtaagca gtacggtgaa
     9121 gcgtttgaaa aacgccacgg catccgtctg ggctttatgt ccttctacgt gaaagcggtg
     9181 gttgaagccc tgaaacgtta cccggaagtg aatgcttcta tcgacggcga tgacgtggtt
     9241 taccacaact atttcgacgt cagcatggcg gtttctacgc cgcgcggcct ggtgacgccg
     9301 gttctgcgtg atgtcgatac cctcggcatg gcagacatcg agaagaaaat caaagagctg
     9361 gcagtcaaag gccgtgatgg caagctgacc gttgaagatc tgaccggtgg taacttcacc
     9421 atcaccaacg gtggtgtgtt cggttccctg atgtctacgc cgatcatcaa cccgccgcag
     9481 agcgcaattc tgggtatgca cgctatcaaa gatcgtccga tggcggtgaa tggtcaggtt
     9541 gagatcctgc cgatgatgta cctggcgctg tcctacgatc accgtctgat cgatggtcgc
     9601 gaatccgtgg gcttcctgat aacgatcaaa gagttgctgg aagatccgac gcgtctgctg
     9661 ctggacgtgt agtagtttaa gtttcacctg cactgtagac aggataaggc attatcgcct
     9721 tctccggcaa ttgaagcctg atgcgacgct taacgcgtct tatcaggcct acgggaagca
     9781 ccaatgtagg tcggataagg cgcttgcgcc gcatccgaca agcgatgcct gatgcgacac
     9841 ttacgcgtct tatcaggcct acaggtgacc gacaatgccc ggaggcgata cgaaatattc
     9901 ggtctacggt ttaaaagata acgattactg aaggatggac agaacacatg aacttacatg
     9961 aatatcaggc aaaacaactt tttgcccgct atggcttacc agcaccggtg ggttatgcct
    10021 gtactactcc gcgcgaagca gaagaagccg cttcaaaaat cggtgccggt ccgtgggtag
    10081 tgaaatgtca ggttcacgct ggtggccgcg gtaaagcggg cggtgtgaaa gttgtaaaca
    10141 gcaaagaaga catccgtgct tttgcagaaa attggctcgg caagcgtctg gtaacgtatc
    10201 aaacagatgc caatggccaa ccggttaacc agattctggt tgaagcagcg accgatatcg
    10261 ctaaagagct gtatctcggt gccgttgtag accgtagttc ccgtcgtgtg gtctttatgg
    10321 cctccactga aggcggcgtg gaaatcgaaa aagtggcgga agaaactccg cagctgatcc
    10381 ataaagttgc gcttgatccg ctgacaggcc cgatgccgta tcagggacgc gagctggcgt
    10441 tcaaactggg tctggaaggt aaactggttc agcagttcac caaaatcttc atgggtctgg
    10501 cgaccatttt cctggagcgc gacctggcgt tgatcgaaat caacccgctg gtcatcacca
    10561 aacagggtga tctgatttgc ctcgacggca aactgggcgc tgatggcaac gcactgttcc
    10621 gccagcttga tctgcgcgaa atgcgtgacc agtcgcagga agatccgcgt gaagcacagg
    10681 ctgcacagtg ggaactgaac tacgttgcgc tggacggtaa catcggttgt atggttaacg
    10741 gcgcaggtct ggcgatgggt acgatggaca tcgttaaact gcacggcggc gaaccggcta
    10801 acttccttga cgttggcggc ggtgcaacca aagaacgtgt aaccgaagcg ttcaaaatca
    10861 tcctctctga cgacaaagtg aaagtcgttc tggttaacat cttcggcggt atcgttcgtt
    10921 gcgacctgat cgctgacggt atcatcggcg cggtagcaga agtgggtgtt aacgtaccgg
    10981 tcgtggtacg tctggaaggt aacaacgccg aactcggcgc gaagaaactg gctgacagcg
    11041 gcctgaatat tattgcagca aaaggtctga cggatgcagc tcagcaggtt gttgccgcag
    11101 tggaggggaa ataatgtcca ttttaattga taaaaacacc aaggttatct gccagggctt
    11161 tactggtagc caggggactt tccactcaga acaggccatt gcatacggca ctaaaatggt
    11221 tggcggcgta accccaggta aaggcggtac tacccacctc ggcctgccgg tgttcaacac
    11281 cgtgcgtgaa gctgttgctg ctactggcgc taccgcttct gttatctacg taccagcacc
    11341 gttctgcaaa gactccattc tggaagctat tgatgcaggc atcaaactga ttatcaccat
    11401 cactgaaggc atcccgacgc tggatatgct gactgtgaaa gtgaaactgg acgaagccgg
    11461 cgttcgtatg atcggcccga actgcccggg cgttatcact ccgggtgaat gcaaaatcgg
    11521 tatccagccg ggtcacattc acaaaccggg taaagtgggt atcgtttccc gttccggtac
    11581 actgacctat gaagcggtta aacagaccac ggattacggt ttcggtcagt cgacctgtgt
    11641 tggtatcggc ggtgacccga tcccaggctc taactttatc gacatcctcg aaatgttcga
    11701 aaaagatccg cagaccgaag cgatcgtgat gatcggtgag atcggcggta gcgctgaaga
    11761 agaagcggca gcgtacatca aagagcacgt taccaagcca gttgtaggtt acatcgctgg
    11821 tgtgactgcg ccgaaaggca aacgtatggg ccacgcgggt gcgatcattg ccggtgggaa
    11881 agggactgcg gatgagaaat tcgctgctct ggaagccgca ggcgtgaaaa ccgttcgcag
    11941 cctggcggat atcggtgaag cactgaaaac tgttctgaaa taaatatctg taataagaaa
    12001 tagccctcgc cgcttccctc tacaggaatg gcgaagggct gtcggtttcg acatggttgg
    12061 ccatcgtatg atggcctttt ttgtgcttat cgcgatgatt ttcgctgcgc tatcagggta
    12121 aatttatagt catcggtatt aaaagcgttg cggctatatt caaacacccg accatcaact
    12181 aaatatccac gcgatacttt ttcaagaatc ggctttgtct ggctgatatt aagcagacgg
    12241 ctcatctctt cggttggcat cagaggaatg atttcctgtt cgctacgatc gataaccatc
    12301 ttcttcactt cttcaataaa gtgatatttc gaattttcca tgacctgcca ggtgagatcc
    12361 gggaacaacg caagcggcat ccaggtttct tccagcgcca ttggcttttg cttgcgatag
    12421 cgcacgcgct tcacatgcca cacacgatcc tgcgcggtga tttgtagctg ttgctgaaga
    12481 aaatcgtcag ccggaatcac ttcaaatatc aggacttcac tgtgtgtatc gacgtgacgg
    12541 tccgacagtt tttcatcaaa actggttaac tgaaaaatat cgtaattgac ccgctcttct
    12601 ttgacgtaag tcccgctgcc ctgaatgctt tcgaggatct gctgctcgac tagctggcgc
    12661 aaagcctgac gcaccgtaac ccggctgacg ccaaactctg tttgtagcgc tgattcagtg
    12721 ggtaacgcat cgccaggttt aagctcgcca cgcgcaattt gttcacgaat gcgatcggca
    12781 atctgccggt ataagggctt gtgtcccatt tttagtatct cattaatacg aatttagcca
    12841 ttatgcccga taaattcatc ctgtaaataa tgcaaataca atacaaataa tttcaatcaa
    12901 gtgaaattga tcatataatg gtattgtttt atcgggcaca ctggcgcgac tataaaaacg
    12961 atcaagtgag gatcatgatg aatctgacga ctctgaccca ccgcgatgcg ttgcgtctga
    13021 atgcgcgctt taccagccgt gaagaggcca tccacgcgtt gactcaacgt cttgctgctc
    13081 tggggaaaat ttccagtact gagcaatttc tggaagaagt gtatcgccgt gaaagccttg
    13141 gcccgaccgc cttaggtgaa gggttggctg tgccgcatgg caaaactgct gcggtaaaag
    13201 aagcggcgtt tgcggtcgcc acactcagcg agccgcttca gtgggaaggc gttgatggcc
    13261 cggaagcagt tgatttagtg gtgctgctgg cgattccccc caatgaagcg ggtactatgc
    13321 atatgcaact gctgacagcg ctgaccacgc gccttgcgga tgatgagatt cgggcgcgta
    13381 tacagtcggc gacgacgcct gatgagttgc tctcggcgct ggatgacaag ggaggcacgc
    13441 aaccttctgc ctctttttcc aacgcgccaa ctatcgtctg cgtaccggcc tgtccggcgg
    13501 gtattgctca cacctatatg gctgcggaat atctggaaaa agccggacgc aaactcggcg
    13561 taaatgttta cgttgaaaaa caaggcgcta acggcattga agggcgttta acggcggatc
    13621 aactcaatag tgcaaccgcc tgtatttttg cggctgaagt cgccatcaag gagagtgagc
    13681 gttttaatgg cattcccgcg ctttcagtgc ctgttgccga gccgattcgc catgcagaag
    13741 cgttgatcca acaagcgctt accctcaagc gtagcgatga gacgcgtacc gtacagcaag
    13801 atacgcaacc ggtgaaaagt gtcaaaacgg agctgaaaca ggcactgttg agcggaatct
    13861 cttttgccgt accgttgatt gtcgcggggg gcacggtgct ggcggtcgcg gtattactgt
    13921 cgcaaatctt cgggctacaa gatctgttta atgaagaaaa ctcctggctg tggatgtacc
    13981 gcaagctggg cggcgggctg ctcggaattt tgatggtacc ggtgctcgcg gcctataccg
    14041 cctattctct ggcagataaa ccggcgttag cgccaggctt tgcggctgga cttgccgcca
    14101 acatgatcgg ctccgggttt ctcggcgcgg tcgttggcgg attgatagcc ggttacttga
    14161 tgcgctgggt gaaaaatcac ttgcgtctta gcagtaaatt caatggattc ctgacttttt
    14221 atctctaccc ggtgctcggt acgttgggag cgggcagtct gatgctgttt gtggtgggag
    14281 aacctgtcgc atggatcaat aactcgctta ccgcctggct gaacggtctg tcaggaagta
    14341 acgcgctgtt gctgggtgcc attttcggtt ttatgtgttc ctttgatctt ggagggccag
    14401 tgaacaaagc cgcttatgca ttctgcctgg gcgcaatggt gaacggcgtt tacggcccgt
    14461 atgccatttt cgcctccgtc aaaatggttt cggcatttac cgtaaccgct tccacgatgc
    14521 tcgcaccgcg cctgtttaaa gagtttgaaa ttgagaccgg gaaatccacc tggctgttag
    14581 ggctggtagg tattaccgaa ggggcgatcc cgatggcgat tgaagatccg ctgcgggtta
    14641 ttggttcgtt tgtgctgggc tctatggtaa cgggcgctat tgtcggtgcg atgaatatcg
    14701 gcctttcgac acccggtgcc ggcattttct cgctcttttt acttcatgat aatggcgcgg
    14761 gcggtgttat ggcggcaatt ggctggtttg gtgcggcatt ggtgggggct gcaatctcga
    14821 ctgcaattct cctgatctgg cggcgtcacg cggttaagca tggcaactat ctgactgatg
    14881 gcgtaatgcc ataaacaaaa aggaaacgac gatgaaagcg gtatctcgcg ttcacatcac
    14941 cccgcatatg cactgggatc gagagtggta tttcaccacc gaagagtcac gtattctgct
    15001 ggtcaataat atggaagaga tcctgtgccg actggaacag gacaacgaat acaaatatta
    15061 cgtactcgac gggcaaacgg cgatcctcga agattatttc gcggtgaaac cggaaaacaa
    15121 agaccgtgtg aagaaacagg tagaagccgg caagttgatt atcggcccct ggtataccca
    15181 gaccgatacc acgattgttt ctgcggaatc catcgtccgt aatctgatgt acggaatgcg
    15241 tgactgcctc gcgtttggcg agccgatgaa aataggttat ttaccagatt cctttggcat
    15301 gtccgggcaa ctgccgcata tctacaatgg atttggcatt acccgcacca tgttctggcg
    15361 cggatgttcg gagcgccacg gtactgataa aaccgagttt ttgtggcaaa gcagtgacgg
    15421 tagcgaagtg acggcgcagg tgctgccgct gggctacgcc ataggtaagt acttacctac
    15481 cgacgaaaac ggattacgta aacgcctcga cagttatttt gacgtgctgg aaaaagcgtc
    15541 tgtaaccaaa gagattttgc tgccgaatgg tcatgaccag atgccattgc agcaaaatat
    15601 cttcgaagtg atggataagc tacgtgagat ctaccctcaa cgtaagtttg tgatgagccg
    15661 ctttgaagag gtatttgaga agatcgaagc gcagcgtgaa agtctggcca ccctgaaagg
    15721 tgaatttatc gatggcaaat atatgcgcgt acatcgcacc atcggttcca cgcgcatgga
    15781 tatcaaaatt gcccacgcac gtattgaaaa taagattgtt aatctgctgg aaccgctggc
    15841 aacgctggcc tggacgttgg gttttgaata ccaccacggc ttgctggaga aaatgtggaa
    15901 agagatctta aaaaatcatg cccacgacag tatcggttgt tgttgcagtg acaaagttca
    15961 tcgcgaaatt gtcgcccgct tcgaactggc tgaagacatg gcggataatc tgctgcgttt
    16021 ctacatgcgc aaaattgccg ataacatgcc acagagtgat gccgacaaac tcgtgctgtt
    16081 taacctgatg ccctggccgc gggaagaagt tattaacacc actgtgcggc tgcgcgccag
    16141 ccagtttaat ctacgagacg atcgcggtca gcctgtaccg tattttattc gccatgcccg
    16201 tgagatcgat ccaggcctaa tcgatcggca aatagttcat tacggtaatt acgatccctt
    16261 tatggagttt gatatacaga tcaaccagat tgtcccttct atgggctatc gcacgcttta
    16321 tatcgaagcg aatcagcctg gcaacgtgat tgcggcaaaa agtgacgctg aagggatact
    16381 ggaaaatgct ttctggcaaa ttgcgctcaa tgaggatggt tctctgcaac tggtagataa
    16441 agacagcggt gtgcgctatg accgagtatt gc
//
LOCUS       DAJVUJ010000095        16371 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.095, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000095 DAJVUJ010000000
VERSION     DAJVUJ010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16371
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.095"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..190
                     /locus_tag="OQQ48_002669"
     CDS             <1..190
                     /locus_tag="OQQ48_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1953384.1"
                     /translation="DMLNPTKDTNWNSTCIYKSRHKMLPVNLTQETLFNSKSQDKHAL
                     FPIFTASWRAYRIMNKGA"
     gene            413..910
                     /gene="ubiC"
                     /locus_tag="OQQ48_002670"
     CDS             413..910
                     /gene="ubiC"
                     /locus_tag="OQQ48_002670"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HCS1953385.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            923..1795
                     /gene="ubiA"
                     /locus_tag="OQQ48_002671"
     CDS             923..1795
                     /gene="ubiA"
                     /locus_tag="OQQ48_002671"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HCS1953386.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(1950..4373)
                     /gene="plsB"
                     /locus_tag="OQQ48_002672"
     CDS             complement(1950..4373)
                     /gene="plsB"
                     /locus_tag="OQQ48_002672"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HCS1953387.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPSHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4544..4912
                     /gene="dgkA"
                     /locus_tag="OQQ48_002673"
     CDS             4544..4912
                     /gene="dgkA"
                     /locus_tag="OQQ48_002673"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCS1953388.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5022..5630
                     /gene="lexA"
                     /locus_tag="OQQ48_002674"
     CDS             5022..5630
                     /gene="lexA"
                     /locus_tag="OQQ48_002674"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HCS1953389.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            5703..7028
                     /gene="dinF"
                     /locus_tag="OQQ48_002675"
     CDS             5703..7028
                     /gene="dinF"
                     /locus_tag="OQQ48_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HCS1953390.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHALWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            7144..7353
                     /locus_tag="OQQ48_002676"
     CDS             7144..7353
                     /locus_tag="OQQ48_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCS1953391.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7395..7910)
                     /gene="zur"
                     /locus_tag="OQQ48_002677"
     CDS             complement(7395..7910)
                     /gene="zur"
                     /locus_tag="OQQ48_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HCS1953392.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQSTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            8228..9213
                     /locus_tag="OQQ48_002678"
                     /pseudo
     CDS             8228..9213
                     /locus_tag="OQQ48_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 domain-containing protein"
     gene            9576..10613
                     /gene="dusA"
                     /locus_tag="OQQ48_002679"
     CDS             9576..10613
                     /gene="dusA"
                     /locus_tag="OQQ48_002679"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HCS1953393.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECGMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDAYPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            10747..10989
                     /gene="pspG"
                     /locus_tag="OQQ48_002680"
     CDS             10747..10989
                     /gene="pspG"
                     /locus_tag="OQQ48_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HCS1953394.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(11154..12137)
                     /locus_tag="OQQ48_002681"
     CDS             complement(11154..12137)
                     /locus_tag="OQQ48_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HCS1953395.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGSGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            12220..13635
                     /gene="dnaB"
                     /locus_tag="OQQ48_002682"
     CDS             12220..13635
                     /gene="dnaB"
                     /locus_tag="OQQ48_002682"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCS1953396.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            13688..14767
                     /gene="alr"
                     /locus_tag="OQQ48_002683"
     CDS             13688..14767
                     /gene="alr"
                     /locus_tag="OQQ48_002683"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCS1953397.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAGAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            15020..16213
                     /gene="tyrB"
                     /locus_tag="OQQ48_002684"
     CDS             15020..16213
                     /gene="tyrB"
                     /locus_tag="OQQ48_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HCS1953398.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNLGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
BASE COUNT         3913 a         3902 c         4333 g         4223 t
ORIGIN      
        1 tgatatgttg aatcctacaa aagataccaa ttggaattct acgtgtatct ataaatccag
       61 acataagatg ttgcctgtta atcttactca ggaaacactt ttcaactcca aatctcagga
      121 taaacatgcg cttttcccca tttttactgc gagttggcga gcttatcgta taatgaataa
      181 gggtgcttaa gtaaaggaaa acatcaccgt tcctggcatc ctgggcggtg atgcccctat
      241 ggttgccctc gccagcgcgg gcatcggcaa agcgtaaggt tcaaaagcga tttaccactt
      301 catgcgattg ttgcgttttt gttgcgcatt agatcactta atttgcttta catctcccgt
      361 aaacactttt ctgcgataca atgcctttac gttatgtaac ggagagttcg gcatgtcaca
      421 ccccgcgtta acgcaactgc gtgcgctgcg ctattttaaa gagatccctg ccctggatcc
      481 gcaactgctc gactggctgt tgctggagga ttccatgaca aaacgttttg aacagcaggg
      541 aaaaacggta agcgtgacga tgatccgcga agggtttgtc gagcagaatg aaatccccga
      601 agaactgccg ctgctgccga aagagtctcg ttactggtta cgtgaaattt tgttatgtgc
      661 cgatggtgaa ccgtggcttg ccggtcgtac cgtcgttcct gtgtcaacgt taagcgggcc
      721 ggagctggcg ttacaaaaat tgggtaaaac gccgttagga cgctatctgt tcacatcatc
      781 gacattaacc cgggacttta ttgagatagg ccgtgatgcc gggctgtggg ggcgacgttc
      841 ccgcctgcga ttaagcggta aaccgctgtt gttaacagaa ctgtttttac cggcgtcacc
      901 gttgtactaa gaggaaaaaa atatggagtg gagtctgacg cagaataagc tgctggcgtt
      961 tcatcgctta atgcgtacgg ataagccaat tggcgcgtta ctgctgctct ggccaacatt
     1021 atgggcgttg tgggtggcga caccgggcgt tcctcagctc tggatcctgg cggtatttgt
     1081 cgcgggtgtc tggctgatgc gcgctgccgg atgtgtggtg aatgattatg ctgaccgaaa
     1141 gtttgatggt catgttaagc gcacggcgaa ccgaccactt cccagcggcg cggtaacaga
     1201 gaaagaggcg cgcgcgctgt ttgtagtgct ggtactgatt tcgtttttac tggtactgac
     1261 gctgaatacg atgaccattc tgttgtcgat tgccgctctg gcgttggcgt gggtgtaccc
     1321 gtttatgaag cggtataccc atctcccgca agtggtgctg ggcgcggcgt ttggctggtc
     1381 gattccaatg gcttttgccg ctgtgagtga gtcggtgcca ttgagttgct ggttaatgtt
     1441 cctcgccaat attctctggg cggtggctta cgacacgcag tatgcgatgg ttgaccgcga
     1501 tgatgatgtg aagattggca ttaaatccac ggcaatcctg ttcggccaat acgataaatt
     1561 gattattggt attttgcaga ttggcgtact ggcactgatg gcgatcatcg gtgagttaaa
     1621 tggcttaggc tggggatatt actggtcaat tctggtggct ggcgcgctgt ttgtttatca
     1681 acaaaaactg attgccaacc gcgagcgtga agcctgcttt aaagcattta tgaataataa
     1741 ctatgttggt ctggtactat ttttagggct ggcaatgagt tactggcatt tctgatgatg
     1801 taaaaaagcc ggatgatcat ccggctttct tctgggttgc ctgatgcgcg gcgcttctca
     1861 ggcctacaca acacatcgca atttattgaa tttgcagatt atggaatgcc ggataaggcg
     1921 ttttcgccgc atccggcaat tctctctggt tacccttcgc cctgcgtcgc actctcaatc
     1981 gtcaaacgca cgtctgatgt aatcaactcc gccagcaact gataaacctt catcgtttct
     2041 gccggttcgg catcgccgct atcgctgata tacccttcat cacgcagcgt cagcaccaga
     2101 gaactgaaca ccgccttgtc gaagaactcc ggcgcgttga taccgtgcag cacggagaga
     2161 cgttgcgcga cggtgcggct ctctttctcc agcgtaccgc ggttgatcga cgggttagcg
     2221 ctcaacagcc agaaggtgat ggcataacgt tgcagcgttt cgcgcgcgcc tgcggccagc
     2281 agctgtagcg tgcgagaatg cgacgggttg atatgcaact catcatcttg cagggtaatc
     2341 agcccctgac gttgcatctc atttgccaac gcatcaataa cgtccggcaa ctcgtcgcga
     2401 tcccagcgca ggaacagctc cgctttcagc attgggtaaa gcacattgac gtgctccatc
     2461 aatacgtcgc gggagatgtg gcgatgctgg gtgacaattg ccgccatcag cgaaggcagc
     2521 accaacatat gcgcaatgtt gttgcgatag taggtcatca gcaccgcttg ctcgcgcggc
     2581 agaatgatga tgtcgccgat agtgtctttc tcaacttcaa acttgttcat ttgcagcgcg
     2641 tgatcgataa gctcgctggc gctggctgaa ggaacggtag agtccgtgga gtaaggtacg
     2701 ttgcgcatca gatccaggta gcagttgagt tgctcggtta actgctcgcg ggtaagtgag
     2761 cgctgacgtg atgccagtag cgcggtacag cagaggttca tggcgtttgc cgcgcctgcg
     2821 ttgttaatgc gtaccatcag atcggcagca atattattga ccgtcggcgt taaccatgcc
     2881 ggacgcaccg cttcgatggg atcgatagat tcacgccagt caggtacatg ctggttaagg
     2941 taggtcatca acggcattgg ttcaccgaag ttgacgtaac cctgaccgag attacgcagc
     3001 ttgcttaaac cgcgcagcat ctgcggcagg ctctctttct ctttcgtcgc accgcgcagt
     3061 tctttggcgt aagtacccac ttccatgacg tgctcataac cgatatagat cggaatcagc
     3121 gtaatcggac gcgtgccgcc acgcagcatc gcctgaatgg tcatcgacag cgtaccggtt
     3181 ttcggatcca gcaaacgccc cgtacgggaa cgaccgcctt ccacgaaata ctcgacggaa
     3241 taaccacggc tgaacagttc gccgagatac tcacggaaaa cggtggaata aagtttattg
     3301 cctttaaacg tacggcgaat aaagaacgcc cccagacggc ggaaaatcgg cccggccggc
     3361 cagaaattca ggttgatccc ggcggcgata tgcggcggca ccagcccctg gtggtacagc
     3421 acgtaagaaa gtagcaggta gtccatgtga ctgcggtggc aaggcacata caccagctca
     3481 tgaccgtcgt gggccagctg gcgaacgcgc tcggcgttat ggacgttgat gccctgataa
     3541 agtcggttcc aggtgaagcc cagaatgcgg tcagtcaggc ggatcatctc gtaagagaaa
     3601 ttcgccgcaa tctcttccat cagcgcaatc gcgttctgct gcgctttttc atgggagatt
     3661 tttttgctgc gcgcttcatc ttctaccgct ttggcaatgg cgcgggaggc gagcagctta
     3721 ttaaacagat cctgacgagc aggcagacgt gggcccacag cagccagacg ttgacgggca
     3781 aagtgcatac gcgccacgcg cgccagtttc tgtgcgatag ttttatccgt gccgtgttca
     3841 tccgccatac ggcgcagcga aactgacggc gagaaacgca caaaactgtc gcgaccgagc
     3901 cacagtacag cgaaaaattt ctgtacgccg ttaagcatac gcagcggcgg gtttacttcg
     3961 cctttttcac gccccggcgc gcgaccaaac atcaccgata ctggcaccat ctgcacatcc
     4021 agatttgggt tgctacggtg caaatcgaga tagtcgtgga acagcttaat agactcttct
     4081 ttcggcgtgt aataggtgaa tacacgcggc ccgccgtgaa tgaacacata gcgcggcagt
     4141 agcgtgccgt cgatttccag cggctctaac gggtcaggca agtcatgagc cagacactgg
     4201 gcgcgtaacg tcagcaaatc tgctttcgag ttgtacggta aaacgtacat aattggacga
     4261 gaggtatcca gccccagttc cggggcagga tctgccggaa tagacttgct ttttaccagg
     4321 atgcttaatg gtaaattcag taatttgtag taaattcgtg gccagccgga cataaacgat
     4381 gtaaagcctc tggttaataa tgcaaatgcg cggcaaggat agcagaaagt catgggaaat
     4441 tctgtggtat ccgctcatgt ttcgcgcggc gttacgcaaa cccgaatcat cggatttaac
     4501 ggtacactga tattgacgct cataatgtaa aaaggttctt tcaatggcca ataataccac
     4561 tggattcacc cgaattatca aagctgctgg ctattcctgg aaaggtttac gcgctgcatg
     4621 gatcaacgaa gcggcattcc gtcaggaagg cgtagcggta ttgttggcgg tggtcatcgc
     4681 ctgctggctg gatgtggacg caattacccg cgtgctgctt atcagctccg tgatgctggt
     4741 gatgattgtg gaaatcctca atagcgccat cgaagcagtg gttgaccgaa ttggctctga
     4801 ataccatgag ctttccggac gcgcaaaaga tatgggatcc gccgcggtgc tgattgcgat
     4861 tatcgtcgcc gtgataacct ggtgcatcct gttatggtcg cattttggat aacccttcca
     4921 gaattcgata aatctctggt ttattgtgca gtttatggtt ccaaaatcgc cttttgctgt
     4981 atatactcac agtataactg tatatacacc cagggggcgg aatgaaagcg ttaacggcca
     5041 ggcaacaaga ggtgtttgat ctcatccgtg atcacatcag ccagacaggt atgccgccga
     5101 cgcgtgcgga aatcgcgcag cgtttggggt tccgttcccc aaacgcggct gaagaacatc
     5161 tgaaggcgct ggcacgcaaa ggagttattg aaattgtttc cggcgcatca cgcgggattc
     5221 gtctgttgca ggaagaggaa gaagggttgc cgctggtagg tcgtgtggct gccggtgaac
     5281 cgcttctggc gcagcagcat attgaaggtc attatcaggt cgatccttcc ttattcaagc
     5341 cgaatgctga tttcctgctg cgcgtcagcg ggatgtcgat gaaagatatt ggcattatgg
     5401 atggcgactt gctggcagtg cataaaactc aggatgtacg taacggtcag gtcgttgtcg
     5461 cacgtattga tgacgaagtt accgttaagc gcctgaaaaa acagggcaat aaagtcgaac
     5521 tgttgccaga aaatagcgag tttaaaccaa ttgtcgttga ccttcgtcag cagagcttca
     5581 ccattgaagg gctggcggtt ggagttattc gcaacggcga ctggctgtaa catatctctg
     5641 agaccgcgat gccgccaggc gtcgcggttt gtttttcatc tctcttcatc aggcttgtct
     5701 gcatggcatt cctcacttca tctgataaag cactctggca tctcgcctta cccatgattt
     5761 tctccaatat caccgttccg ttgctgggac tggtcgatac ggcggtaatt ggtcatcttg
     5821 atagtccagt ttatttgggc ggcgtggcgg ttggtgcaac ggcgaccagc tttctcttta
     5881 tgctgttgct gtttttacgc atgagcacca ccgggctgac tgcgcaggct tatggtgcca
     5941 aaaatcctca ggcattagcc cgtgcgctgg tgcaaccgtt gctgttggcg ttgggggcag
     6001 gggcgttaat tgcgctgctg cgtacaccga ttatcgatct ggcactgcat attgttggcg
     6061 gtagtgaagc agtcctggaa caggcgcggc gctttcttga aatccgctgg ttaagcgcac
     6121 cggcgtcact ggcgaatctg gtattacttg gttggttact cggcgtgcaa tatgcccgtg
     6181 cgccagtaat tttgttagtg gtcggcaata tcctcaacat tgtgctggat gtctggctgg
     6241 tgatggggct gcatatgaac gtgcagggcg cggcgctggc gacggttatt gcggaatatg
     6301 caacattgct gattggtctg ctaatggtgc gtaaaatcct caaactacgc ggaatttccg
     6361 gcgaaatgct gaaaactgcc tggcgaggaa acttccgtcg cttgctggcg cttaaccgcg
     6421 atatcatgct gcgttcgctg ttgttgcaac tctgtttcgg cgcgatcacc gtacttggtg
     6481 cgcgactggg gagtgacatt atcgctgtta acgcggttct gatgacgcta ctcaccttta
     6541 ccgcctatgc gctggatggt tttgcctacg cggttgaagc gcattccggt caggcgtacg
     6601 gtgcgcgcga cggtagccag ttgctggatg tctggcgggc agcgtgccgc cagtcgggga
     6661 tcgtagcgtt actgttttcg gtagtttatt tgctggcagg ggaacacatc attgcgttgc
     6721 tgacgtcgtt aacccagatt cagcagctgg ccgaccgcta tcttatctgg caggtgattt
     6781 tgccgttggt tggcgtctgg tgttatctgc tggacggcat gtttataggc gcaacgcgcg
     6841 cagccgaaat gcgtaacagt atggcggtgg ccgccgcagg ttttgcgctg acgctcctta
     6901 cgctgccgtg gctgggtaat catgctttgt ggctggcgtt aaccgtcttt ctggcgttgc
     6961 gcgggctttc tctggcggct atctggcggc gtcactggcg caacggtacc tggtttgccg
     7021 caacgtgacg gttaaaaatt ctgaataaat aatcctaagc caaattgctg actacactta
     7081 atctcacgtt cagaagaaaa gtgaacgtac tctcattcac aacctaacga tgaggtcttg
     7141 attatgaata aagatgaagc cggcggtaac tggaaacagt ttaaaggtaa agtgaaagag
     7201 caatggggca aactgaccga tgatgatatg acgatcattg aaggtaaacg tgatcaactg
     7261 gtcggtaaaa tccaggaacg ttatggttat cagaaagatc aggcagaaaa agaggtcgtg
     7321 gattgggaaa cccgcaatga atatcgctgg taattaatcc ctcctgcccg acgtgtacaa
     7381 ggatgtacgc cctcttaacg cggtttcttt ttcacctgca cagagtgatc atgctggcac
     7441 tgttcaggat gacgacacgc ttccacttcc acgcatgccg cacagagccc atgtgcttca
     7501 atcacattat gccgcagggc aaaccccatt tttgccgcca gcgtatgcat gatgtcttcc
     7561 acgccttctg cacactcttc tttcactgcg ccgcagcgat cgcaaataaa catggctgaa
     7621 gtatgggtgg actggtcgaa cagatgacag agcacataac tgttggtgga ttccacctta
     7681 tgcacaaaac cttgctcaag cagaaaatcc agcgcgcgat aaaccgttgg cggcttggct
     7741 tgcggttcag cttcgcgcag taaatcaagc agatcataag cgctgatagc gccatcctgc
     7801 agactcatca ggcgcaacac ttccaggcgc tgtggggtca ggcgcacatt acgctgcgcg
     7861 cagatttttt cagcctgcgc taataactcc tgcgttgtgg tcttttccat cgggcacctc
     7921 aaagtggggg ttaagaatgt ccttacttta ccatgttcca ggaaaaacac cgagaaccct
     7981 tattgttgcc gtaatgttga ttttctgttt tgtaggtaag gtgttatgtt gccttgtcgt
     8041 accattatca acacgataat aattaataat tcatttttaa ataaattgct tcaagttaac
     8101 tcgctattta tttaactggg tttaaacgca tacctgtagt tcagaaatta tgagcgattt
     8161 ttgatagtga ccagatggca aaagaattat aaggaggaca cggtacgaca aaaaaggtag
     8221 atgaatcatg ttgaagataa tacccggtgc aactggatac tttaataaaa cactcaattc
     8281 aaatcaattc gataatgaag atgcaatcaa agataaatta gataatagag gttcaatcaa
     8341 aggtaaatta aataatatat atggtaaatc tattgattat gcagcgctgc gtcatcggga
     8401 tataattatt gcaaaaatag atttgtttat tcaacgaatc acgcataatt tatggcatgc
     8461 acgtaaaaaa atgtgttttt aattgagcaa ataaatgatc tcaaaatgtg ggtcaataag
     8521 tatattgacg attgcactga tgaggattta aacgatcgtg actttattgc atcagttgtt
     8581 gaccgggcta tttttcattt cgcgattaat agtatatgta atcctgggga taataaagat
     8641 gcgactccca ttgaacaatg tacttttgat gtagaaacta agaatggcct tccctccacg
     8701 gttcagctat tttatgagga atctaaggat aatgaacctt tagcgaatat acattttcaa
     8761 acaataggtt ctggtttttt aacgtttgtt aatgcctgcc aggaacatga tgacaacagc
     8821 ttaaaattat ttgcttcgct gttaatttca ctttcatatt ctagtgccta cgcagattta
     8881 tcagaaacag tgtatattaa tgaaaataat gagagctacc tgaaagctca gtttgaaaaa
     8941 ttatctcaac gtgatatgaa gaagtacctg ggagagatga agcgtctggc tgatggggga
     9001 gaaatgaatt ttgatggcta tctggataag atgtcacatc tggtgaatga agggacgctc
     9061 gatcctgata ttttaagcaa aatgcgagat gctgcaccac aattaattag cttcgcgaag
     9121 tcgtttgacc caacctcaaa ggaagagatt aaaatactta cagacacttc taaattaatt
     9181 tatgatttgt tcggggttaa atcggagaaa taatatgtga agttcttcga tagtatggaa
     9241 ggcattatat aaaaggaccc aatatttatt gggtcctttt ttctctatca atgctattag
     9301 cagggagata tatcaccaaa gtttaatgtg tgatttttta tttatcgtcg aacctggatt
     9361 gtttatcatt ggccttaaca aagttaacgg ctaataagat tatttccatc acttcgtgag
     9421 agcttcatgc cttgagagga tctcaatttt cttttgcaat gagacaggcg cttcctgttg
     9481 ttatggtata gtaccccgct attgagcctc ctgaatagtg atgctgaata acataacccc
     9541 atgatatatc gataaaataa tctctacatt tgaaaatgca cggtaattct gaaatgcaaa
     9601 aaatcaacca aaccagcgca atgcctgaaa aaactgacgt tcactggagt ggtcggttta
     9661 gcgttgcacc aatgctcgac tggacggaca gacattgccg ctatttcttg cgtctgcttt
     9721 cccgcaatac gttgctgtat accgaaatgg tgaccacagg ggcgattatt cacggtaaag
     9781 gtgattacct ggcgtacagt gaagaagaac atccggtagc gttgcaactc ggcggtagcg
     9841 atccggcggc gctggcacag tgtgcgaagc tggcagaagc gcgtggatat gatgagatca
     9901 acctgaatgt cggctgcccg tctgaccggg tgcagaacgg catgtttggt gcgtgtctga
     9961 tgggtaatgc gcagctggtt gccgattgcg tgaaagcgat gcgcgatgtg gtgtcgattc
    10021 cggtgacggt gaaaacgcgt attggcatcg atgaccagga cagctatgaa tttctctgcg
    10081 atttcatcaa caccgtttcc ggcaaaggcg agtgtgggat gttcatcatc catgcacgta
    10141 aagcctggct ttcggggtta agcccgaaag aaaaccgtga aatcccgccg ctcgattatc
    10201 cgcgtgtgta tcaactgaag cgtgactttc cgcatctgac aatgtcgatt aacggtggta
    10261 tcaagtcgct ggaagaggcc aaagcacacc tgcaacatat ggatggcgtg atggtcgggc
    10321 gcgaggcgta tcagaatccg ggtattctgg cggcggtaga ccgggagatc tttggttcct
    10381 cggataccga tgcctatccg gtggcggtag tgcgcgccat gtatccgtac attgagcgtg
    10441 aactcagcca ggggacgtat ctcggccata ttacccggca tatgttgggc ttgttccagg
    10501 gtattcctgg cgcgcggcag tggcggcgtt atttaagtga aaatgcccat aaagcgggtg
    10561 cagacattaa tgtgctggaa cacgcgctca aactggtggc ggataagcgt taacttttca
    10621 ccaaaaagta gtcaaattca ccacgccctg cgcaccgtcg cggggcgttt tgctgttaaa
    10681 tcaatagatt atttttggca tgattcttgt aatgccagca agagatttca tatttgggag
    10741 agcatcatgc tggaactact ttttgtgatt ggcttttttg tcatgctgat ggtcaccggc
    10801 gtttcgttgc tgggcattat cgccgcgctg gttgtggcga cggccattat gttcctcggc
    10861 ggtatgctgg cattgatgat taagttgctg ccgtggttac tactggcgat tgcggtggtg
    10921 tgggttatta aggcgattaa agcaccaaaa gtgcctaaat atcagcgtta tgaccgctgg
    10981 cgttactaag ggattgtgcg gatgatcaca acctaaggtt ttatccttag aacaaaatag
    11041 gaattgataa tcaaatctgt cactattgcg cctctaacag attcatcgtg ctgtacccta
    11101 catacagccg actataaaaa gaaagggctt cccaggtgga agccctattt cttttatgga
    11161 atcagcaggc tggaaccttg cgtcgcccgg ctttccagaa tctcatgcgc acgctgcgca
    11221 tccttcagcg gatatttctg ctgctcggcg acatcgacct taatcacacc gctggcaatc
    11281 aaagagaaca gttcattact ggcctcggtt aattcctccc gtgtggtgat atagccttgc
    11341 agggaagggc gtgtcacata caacgagcct ttttgattga gaatgcctaa gttcacaccg
    11401 gtaaccgcac ctgatgagtt gccaaaactg accattaagc cgcggcgttg caggcaatcc
    11461 agcgaccgtt cccaggtgtc tctgcccacg gaatcgtaca ctacgcgcac tttcttaccg
    11521 ccggtgatct ctttcaatcg ctcgaccaga ttctcttcac gatagttaat aacctgccac
    11581 gcgccggctt ttagcgcgct ctgcgctttt tgcgcggttc ctacggtgcc gataagtttc
    11641 gcgcccagag cttttgccca ctggcaggca atcaagccaa cgccgccagc cgctgcgtgg
    11701 aacaggaact gttcatcggg tttaatttca taggttttgc gcagcagata ataaaccgtt
    11761 aagcctttca ggaaagatgc tgcagcttgc tcaaaagaaa tagccgcagg cagaatcgcc
    11821 gctttatccg cattaatgtt atgcacagag ctgtaagcgc ctaacgccga ctgcgcatag
    11881 actacacgat cgcctgcctt aatatgcttt acaccactgc cgactttact cacgatgcct
    11941 gccgcttcgg tacctgatcc gctgggtagt gatggcggcg ggtaaaggcc gctgcggata
    12001 tacgtgtcga taaaattgat gccgatggct ttattttcga cctggatttc attctctgcc
    12061 ggatcggcag gagtgaactc tacggcttga agtacttccg ggccaccgtg cttgtgaaat
    12121 tcaattcgtg ttgccatgtg tcctccagaa cgtatcgtca gggtctgctt catatggtaa
    12181 agtttcgacc cattctttat ctcggtaact ccattcacta tggcaggaaa taaacccttc
    12241 aacaaacagc aggctgaacc ccgcgaacgc gatccacaag ttgccgggct gaaagtgcct
    12301 ccgcactcga tcgaagcgga gcagtcggtg ttgggcggtt taatgctgga taacgaacgc
    12361 tgggacgatg tagccgagcg tgtggtggca gacgattttt acacccgccc acaccgccat
    12421 atctttactg aaatggcgcg tttgcaggaa agcggtagtc ctatcgatct gattaccctt
    12481 gcggaatcgc tggaacgcca ggggcaactc gatagcgtcg gtggttttgc ttatctggca
    12541 gagctgtcaa aaaatacgcc aagtgcggcg aacatcagtg cttatgctga catcgtgcgt
    12601 gaacgtgccg ttgtccgtga gatgatctcg gttgcgaatg agattgctga agccggtttt
    12661 gatccgcagg ggcgtaccag cgaagatctg ctggaccttg ctgaatcccg cgtctttaaa
    12721 attgccgaaa gtcgtgcaaa caaagacgaa gggccgaaga acatcgccga tgtgctcgac
    12781 gcaaccgtgg cgcgtattga gcagttgttt cagcagccac acgatggcgt taccggagta
    12841 aacaccggtt atgacgatct caacaaaaaa accgctggct tgcagccgtc ggatttgatc
    12901 atcgtcgccg cgcgtccgtc gatgggtaaa acaacatttg cgatgaacct cgtcgaaaac
    12961 gcggcgatgt tgcaggataa accagtactt atcttctcgc tggagatgcc ttcagaacag
    13021 attatgatgc gttctctggc gtcgctgtcg cgcgttgacc agactaaaat ccgtaccggg
    13081 cagctcgatg atgaagactg ggcgcgcatt tccggcacca tgggtatttt gctcgaaaaa
    13141 cgcaatatct atatcgatga ctcctccggc ctgacgccaa cggaagtgcg ttcccgcgca
    13201 cgccgtattg cccgtgaaca cggcggcatc gggcttatca tgatcgacta cctgcaactg
    13261 atgcgcgtac cggcgctttc cgataaccgt acgctggaaa ttgcagaaat ctctcgctcg
    13321 ctgaaagcac tggcgaaaga actgaacgtg ccggtggtgg cgctgtccca gttgaaccgt
    13381 tctctggaac aacgtgccga caaacgcccg gtcaactccg acctgcgtga atctggctct
    13441 atcgagcagg atgcggactt gatcatgttt atctatcgtg atgaggtgta tcacgaaaac
    13501 agtgatttaa aaggcatcgc ggaaattatt atcggtaaac aacgtaacgg cccaatcggg
    13561 acggtacgcc tgacctttaa cggtcaatgg tcgcgcttcg acaactatgc ggggccgcag
    13621 tacgacgacg aataataatt attttatgaa ttaggcaatt aaagcaaaca cttatcaagg
    13681 aacacaaatg caagcggcaa ctgttgtgat taaccgccgc gctctgcgac acaacctgca
    13741 acgtcttcgt gaactggccc ctgccagtaa aatggttgcg gtggtgaaag cgaacgctta
    13801 tggtcacggt cttcttgaga ccgcgcgaac gctccccgat gctgacgcct ttggcgtagc
    13861 ccgtctcgaa gaagctctgc gactgcgtgc ggggggaatc accaaacctg tactgttact
    13921 cgaaggcttt tttgatgcca gagatctgcc gacgatttct gcgcaacatt ttcataccgc
    13981 cgtgcataac gaagaacagc tggctgcgct ggaagaggct agcctggacg agccggttac
    14041 cgtctggatg aaactcgata ccggtatgca ccgtctgggc gtaaggccgg aacaggctgg
    14101 ggcgttttat catcgcctga cacagtgcaa aaacgttcgt cagccggtga atatcgtcag
    14161 ccattttgcg cgcgcggatg aaccaaaatg tggcgcaacc gagaaacaac tcgctatctt
    14221 taataccttt tgcgaaggca aacctggtca acgttccatt gccgcgtcgg gtggcattct
    14281 gctgtggcca caatcgcatt ttgactgggt gcgcccgggc atcattcttt atggcgtctc
    14341 gccgctggaa gatcgctcca ccggtgccga ttttggctgt cagccagtga tgtcactaac
    14401 ctccagcctg attgccgtgc gtgagcataa agccggagag cctgttggtt atggtggaac
    14461 ctgggtaagc gaacgtgata cccgtcttgg cgtagtcgcg atgggctatg gcgatggtta
    14521 tccgcgcgcc gctccatccg gtacgccagt gctggtgaac ggtcgcgaag taccgattgt
    14581 cgggcgcgtg gcgatggata tgatctgcgt agacttaggt ccacaggcgc aggacaaagc
    14641 cggggatccg gtcattttat ggggcgaagg tttgcccgta gaacgtatcg ctgaaatgac
    14701 aaaagtgagc gcttacgaac ttattacgcg cctgacttca agggtcgcga tgaaatacgt
    14761 ggattaatcg ttctgtaata tttgattgtc tgtgccggat gcggcgtgaa tgccttatcc
    14821 ggccaataaa atcctaaaaa ttcaataagt tgatgttctt tcatgctctt ataaaggtcg
    14881 tgcctctggc ggatgttcgt ttgtcatgag tctcactctg ttgctaattg ccgttcgctc
    14941 ctgaacatcc actcgatctt cgccttcttc cggtttattg tgttttaacc acctgcccgt
    15001 aaacctggag aaccatcgcg tgtttcaaaa agttgacgcc tacgctggcg acccgattct
    15061 tacgcttatg gagcgtttta aagaagaccc tcgcagcgac aaagtgaatt taagtatcgg
    15121 tctgtactac aacgaagacg gaattattcc acaactgaaa gccgtggcgg aggcggaagc
    15181 gcgcctgaat gcgcagcccc atggcgcttc gctttattta ccgatggaag ggcttaacag
    15241 ctatcgccat gccattgcgc cgctgctgtt tggtgcggac catccggtac tgcaacaaca
    15301 gcgcgtagca accattcaaa cccttggcgg ctcaggggca ttgaaagtgg gcgcggattt
    15361 cctgaaacgc tacttcccgg aatcaggcgt ctgggtcagc gatcctacct gggaaaacca
    15421 cgtagcaata ttcgccgggg ctggattcga agtgagtact tacccctggt atgacgaagc
    15481 gactaacagc gtgcgcttta atgacctgtt ggcgacgctg aaaacattac ctgcccgcag
    15541 tattgtgttg ctgcatccat gttgccacaa cccaacaggt gccgatctca ctaatgatca
    15601 gtgggatgcg gtgattgaaa ttctcaaagc ccgcgagctt atcccattcc tcgatattgc
    15661 ctatcaagga tttagtgccg gtatggaaga ggatgcctac gctattcgcg ccattgccag
    15721 cgctggatta cccgctctgg tgagcaattc gttctcgaaa attttctccc tttacggcga
    15781 gcgcgtcggc ggactttctg ttctgtgtga agatgccgaa gccgctggcc gcgtactggg
    15841 gcaattgaaa gcaacagttc gccgcaacta ctccagcccg ccgaatttgg gtgcgcaggt
    15901 ggtggctgca gtgctgaatg acgaggcatt gaaagccagc tggctggcgg aagtagaaga
    15961 gatgcgtact cgcattctgg caatgcgtca ggaattggtg aaggtattaa gcacagagat
    16021 gccagaacgc aatttcgatt atctgcttaa tcagcgcggc atgttcagtt ataccggttt
    16081 aagtgccgct caggttgacc gactacgtga agaatttggt gtctatctca tcgccagcgg
    16141 tcgcatgtgt gtcgccgggt taaatacggc aaatgtacaa cgtgtggcaa aggcgtttgc
    16201 tgcggtgatg taatgcagga aagcaggctg gagttaccca gcctgcagtg aaattaaact
    16261 gtcgtcgctt tcactatttc tttgtagatg atttttttga tgccgttgtt ctacgtgaga
    16321 gataataaaa cgttgtcagt tcttttattg ttaagtttat cccaattaac t
//
LOCUS       DAJVUJ010000096        15726 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.096, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000096 DAJVUJ010000000
VERSION     DAJVUJ010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15726
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.096"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..1006
                     /locus_tag="OQQ48_002685"
     CDS             <1..1006
                     /locus_tag="OQQ48_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCS1953399.1"
                     /translation="RVIKTFGELCDIWTKIKETELTTNTMKKTKSQLKTLRIIICEST
                     PISHIRYSDILNYRNELLHGETLYLDNPRSNKKGRTVRTVDSYIALLCSLLRFAYQSG
                     FISTKPFEGVKKLQRNRIKPDPLSKTEFNALMESEKGQSQNLWKFAVYSGLRHGELAA
                     LAWEDVDFEKGIVNVRRNLTILDMFGPPKTNAGIRTVTLLQPALEALKEQYKLTGHHR
                     KSEITFYHREYGRTEKQKLHFVFMPRVCNEKQKPYYSVSSLGARWNAAVKRAGIRRRN
                     PYHTRHTFACWLLTAGANPAFIASQMGHETAQMVYEIYGMWIDDMNDEQIAMLNARLS
                     "
     gene            1058..1993
                     /gene="ttcA"
                     /locus_tag="OQQ48_002686"
     CDS             1058..1993
                     /gene="ttcA"
                     /locus_tag="OQQ48_002686"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HCS1953400.1"
                     /translation="MQENQQITKKEQYSLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPVYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(2122..3495)
                     /gene="dbpA"
                     /locus_tag="OQQ48_002687"
     CDS             complement(2122..3495)
                     /gene="dbpA"
                     /locus_tag="OQQ48_002687"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HCS1953401.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAVISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(3941..4924)
                     /gene="zntB"
                     /locus_tag="OQQ48_002688"
     CDS             complement(3941..4924)
                     /gene="zntB"
                     /locus_tag="OQQ48_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HCS1953402.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            5179..6411
                     /gene="dgcM"
                     /locus_tag="OQQ48_002689"
     CDS             5179..6411
                     /gene="dgcM"
                     /locus_tag="OQQ48_002689"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HCS1953403.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRELTETEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISSLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(6432..6995)
                     /gene="smrA"
                     /locus_tag="OQQ48_002690"
     CDS             complement(6432..6995)
                     /gene="smrA"
                     /locus_tag="OQQ48_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HCS1953404.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMV
                     FSFIHQALADGLRNVLIIHGKGREDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(7325..8233)
                     /locus_tag="OQQ48_002691"
     CDS             complement(7325..8233)
                     /locus_tag="OQQ48_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1953405.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEDIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTPSLITQFRRECGYLQS"
     gene            8409..9709
                     /gene="abgA"
                     /locus_tag="OQQ48_002692"
                     /pseudo
     CDS             8409..9709
                     /gene="abgA"
                     /locus_tag="OQQ48_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
     gene            9709..11154
                     /gene="abgB"
                     /locus_tag="OQQ48_002693"
     CDS             9709..11154
                     /gene="abgB"
                     /locus_tag="OQQ48_002693"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HCS1953406.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESAGFTVTRNVGYIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSVTP
                     GENGHGCGHNLLGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANFPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYHALSHFGTPEWNSEELAFTKQIQ
                     ATLPPNDRQNSLNNIAATGGENGKAFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTLNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTLSSLK"
     gene            11191..12717
                     /gene="abgT"
                     /locus_tag="OQQ48_002694"
                     /pseudo
     CDS             11191..12717
                     /gene="abgT"
                     /locus_tag="OQQ48_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate transporter"
     gene            12728..13243
                     /gene="ogt"
                     /locus_tag="OQQ48_002695"
     CDS             12728..13243
                     /gene="ogt"
                     /locus_tag="OQQ48_002695"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCS1953407.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLSDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            13438..14190
                     /gene="fnr"
                     /locus_tag="OQQ48_002696"
     CDS             13438..14190
                     /gene="fnr"
                     /locus_tag="OQQ48_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HCS1953408.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            14342..15292
                     /gene="uspE"
                     /locus_tag="OQQ48_002697"
     CDS             14342..15292
                     /gene="uspE"
                     /locus_tag="OQQ48_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HCS1953409.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFEPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(15342..15600)
                     /locus_tag="OQQ48_002698"
                     /pseudo
     CDS             complement(15342..15600)
                     /locus_tag="OQQ48_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
BASE COUNT         4060 a         3911 c         3856 g         3899 t
ORIGIN      
        1 gagggtcata aaaacattcg gggagctttg tgatatctgg acaaaaatta aagagacaga
       61 gttaacaaca aacacaatga agaaaacgaa atcacaatta aaaacactca ggataataat
      121 ttgtgagagt accccgatat cgcatattcg ttatagcgat atcttaaact accggaatga
      181 actgctgcat ggagaaacgc tttacctaga taatccaaga tccaacaaaa aaggaagaac
      241 cgtgcgcaca gttgatagct atatcgccct gctctgttcg ttgttacgtt ttgcgtatca
      301 gtcgggattt atatcaacca aaccatttga aggagtaaaa aaattacagc gaaacagaat
      361 aaaacctgac ccgttatcta aaacagaatt caatgcatta atggaaagtg aaaaaggaca
      421 gagccaaaac ttgtggaaat ttgccgttta ctccgggctt cgtcacgggg aactggcagc
      481 tctggcgtgg gaggatgtgg atttcgagaa gggaattgtg aatgtcagaa gaaacctgac
      541 gatacttgat atgttcggtc ccccaaaaac aaatgccggg atccggacgg taacattact
      601 gcagccggct cttgaagcac tgaaggagca atacaaactg accgggcatc atcgcaaaag
      661 cgaaatcact ttttatcatc gggagtacgg cagaactgaa aagcaaaaac tgcattttgt
      721 tttcatgccc agggtgtgta acgaaaaaca gaaaccttat tactcggtaa gcagtttggg
      781 tgcaagatgg aatgcagcag taaaacgtgc tggtattcgc cgccgtaatc cgtaccatac
      841 gcgacatact tttgcctgct ggctgttgac ggcaggagcg aacccggcat ttatagccag
      901 ccagatgggg catgaaactg cgcaaatggt gtatgaaatt tacggtatgt ggattgatga
      961 catgaacgac gaacagatag ccatgttgaa tgcgcggtta tcgtagttgc aaagtttgcc
     1021 cccaatttgc cccatttagt accagagaac tgaaataatg caagaaaatc aacaaattac
     1081 aaagaaagaa caatacagcc tgaacaaatt acaaaaacgt ctacgtcgta acgtgggcga
     1141 agccattgct gacttcaata tgattgaaga aggcgatcgc atcatggttt gcctctcagg
     1201 gggtaaagac agctatacca tgctggagat tctgcgcaat ttgcagcaaa gcgcgccaat
     1261 caatttttcg ctggtggctg ttaacctcga tcaaaagcaa ccgggcttcc cagaacacgt
     1321 tctgcccgtg tatcttgaaa agctgggcgt tgagtacaag attgtcgaag agaacactta
     1381 cggtatcgtg aaagagaaga ttccagaggg caaaaccact tgctcactgt gttctcgcct
     1441 tcgtcgcggt atcctttatc gtaccgcaac ggaactgggg gcgacgaaga tcgcgctggg
     1501 tcaccatcgt gacgatatcc tgcagacgtt gttcttaaat atgttctacg gcggcaagat
     1561 gaaaggtatg cctccgaaac tgatgagcga tgatggcaaa catatcgtta ttcgtccgct
     1621 ggcctactgc cgcgagaaag atattcagcg atttgccgat gcaaaagcgt tcccgattat
     1681 tccgtgcaac ctgtgcggtt cacagcctaa cctgcaacgt caggtgattg ctgacatgtt
     1741 gcgtgactgg gataaacgtt atccagggcg tatcgagacg atgttcagcg cgatgcagaa
     1801 tgtggtgccg tcgcatctgt gcgataccaa cctgttcgat ttcaaaggca tcacccacgg
     1861 ttctgaagtg gttaacgggg gtgatctggc gtttgatcgc gaagagatcc cactacaacc
     1921 ggcgggctgg cagccagaag aagatgaaaa tcagttggat gagttacggc tgaatgtggt
     1981 tgaagtgaaa taaccaggat agcgcccgat gcgcaagcgt atcgggctac tcttatggag
     2041 gccggataag acgcggccaa cgtcgcatcc ggcaatgccg aagaagatgt ttactcttgc
     2101 acccggcaat tcaacatttc attattttaa taaccgcacc cggcacgttt ttcctttaat
     2161 cttcccgccc tgtaactgtt tccatgcttt atgagcaaca gcctgacgga ccgcgacata
     2221 gacatgcgcc ggatgcacgg cgattttgcc aatatctgcg ccatcaagcc cgatatctcc
     2281 tgtcagtgca cctaatacat cacccgggcg cattttggct tttttcccgc catcgataca
     2341 caacgttgcc atttctgctt ccagcggcac aatggaacta ttagctggcg gcgtttgcca
     2401 gttaagtttt atctgcaaca tgtcagaaat gatattggcc cgctgtgctt cttccggagc
     2461 acagaaactg atcgccagac cgctatttcc tgcacgagct gtacggccga tgcgatgtac
     2521 atgaacttca gggtcccacg ccagctcaaa gttcaccacc agctcaagcg atttaatatc
     2581 cagaccacgc gcagcaacat cagtcgcaac cagtacacgg gcgctaccgt tagcaaaacg
     2641 taccagggtc tgatcgcgat cgcgttgctc caaatcgccg tgtaatgaca atgcactttg
     2701 ccctacttca ttcagcgcgt cgcagacagc ctggcaatct tttttggtat tgcaaaacac
     2761 cacgcaagag gatggctgat gcaagcttaa taaccgttgc aacagaggaa ttttgccttt
     2821 gctggatgtc tcataaaatt gttgttcaat gggtggcaaa gcatctgttg agtcaatttc
     2881 aatcgccaaa ggatcgcgtt gcactcgtcc gctgattacg gcgatggctt ccggccaggt
     2941 tgccgaaaac agaagcgtct gtcgagatgc aggcgcaaaa cggatgacat catcaatggc
     3001 atcgctaaat cccatatcca gcatgcggtc ggcctcatcc atcaccaaag tattcagcgc
     3061 atccagtgat accgtgcctt tttgcaagtg atccagcaaa cgacccggcg ttgccacgat
     3121 aatatgcggc gcatgttgca acgaatcacg ctgcataccg aacggttgac caccgcacaa
     3181 cgtcaaaatt ttggtatttg gcagaaaacg cgccagccga cgcaattcac ctgccacctg
     3241 atccgccagt tcacgcgtag gacacagcac taaagcctgg gtttgaaata gcgacgcatc
     3301 aatttgctgt aacaagccga ggccaaaagc cgccgttttg ccgctgccgg ttttcgcctg
     3361 cacgcgaaca tcttttccgg caaggatcgc cggaagcgcg gcggcctgca ccggcgtcat
     3421 agttaaataa cccaactcat taaggttcgt gagttgggcg ggaggcaaaa cattcagggt
     3481 agaaaaagcg gtcacaatct attctcgtgg tcatcgacgc aaagttagca ggcgcgtatc
     3541 ctcgcagatc tacgcgggtg gtgggtcagg catgggttgc gggcgaggga tcggatcggg
     3601 cactggaaca ggatcgccag gaatcggttc agggacagga atttgcaaat aaataagtgt
     3661 cgtcatattt ccctccggtc attgggtgga ctcttaaagg gtagacgctg ataaataaca
     3721 ggcaaaaaaa agccgactca tcaaagtcgg cgtcgtacga atcaattgtg ctatgcagta
     3781 attcaaaaaa ggaagtaaga caatatggag cgcaacgccc atcgcttgac gttgcattca
     3841 cctgcaaggg agatattgcc ctgaatgggt agagagttta ttgacttcgc tcaaactttg
     3901 cggcgttttt gtatacagac agccggaaaa attgcttttg ttacaaccat ttactacgat
     3961 gcaaccataa agcaacacca ccaataagaa caactaacag aatacaaaaa attgaaaatc
     4021 cgaattgcca cccgccgcca gggatcccac caaggttgac gccaaataac cctgtcagaa
     4081 aggtactggg taaaaagacc attgccatca atgacattgt ataggtacga cgagctaaat
     4141 tttcctgcat cacctgagcg atttcatccg ccatcacgcc agtccgtgct atacaggcgt
     4201 cgatttcgtc aagaccgcgc ccaaggcgat cggcaatatc ctgcatccga cggcgttggt
     4261 catcgctcat ccacggcaaa cgttcactgg caagacgagc ataaacatca cgttgcggtg
     4321 ccatatagcg acgcatcacg attaattgtt tgcgcagcag agccaggaat ccacgcggtg
     4381 gaatttgctg atcaaggaga ttatcttcaa ggtcgataat tttatcgtgc agctgctcga
     4441 taaattcgct ggaatgatcg gtcaacgcat cgcacacatc caccagccac cctccgcaat
     4501 cggtcggacc cgtgccctct tccagatcgc tcaccacatc gtccagcgcc agcactttgc
     4561 gttgtcgggt cgaaacaatt aaccgcccgt ccatatatac acgcatggcg accagttgat
     4621 cggggcgttc atcggtgctg ccgtttatac agcgcaatgt aatcagcgtg ccttcaccga
     4681 gacggctgac tcggggccgt gtgctctcgc ctgccagcgc atcacgtacg ttattgggaa
     4741 gcagcggtgt tgtcgccagc cattgggcgc tatcatggtg tacataatta aggtggagcc
     4801 agcagggatg cgcttcatca atcacatctg tattttccag cggtttaacg ccgcctctac
     4861 catccagcat ccaggcaaat actgcatccg ggacattaac gtccgatccc ttaatcgctt
     4921 ccacagtgcc tccatcatca acgcattatt ttgtagtcta gccttctggc cctgttacgc
     4981 aacatctcat caccccatta ccctgaaatg attaataaaa ttctgtctaa attgaataca
     5041 aaaagcaaaa tgcttttccg tatacaaacc gtgtgaagtg ttaaatagcg tctatcatta
     5101 tcagaattat ctgatcatat gacgtggctt ttttgcgatc ggatagcaac aaaaattgat
     5161 aaaaataacg ggatctcaat gattacgcac aacttcaata ccctggactt actcaccagt
     5221 cctgtctgga ttgtttcgcc ctttgaggaa cagttaattt atgccaatag cgcggcgcga
     5281 ctgttgatgc aagacctcac gtttagtcag ctacgaaccg gaccctattc cgtctcctca
     5341 caaaaagaac tgccgaaata cctctccgat ctgcaaaacc aacacgatat tatcgaaatc
     5401 ctcactgttc agcgtaaaga agaggaaaca gcattaagct gtcggcttgt tttgcgagag
     5461 ctgacagaaa cagaaccggt gattattttc gaaggtatcg aagcgccggc aacgctgggt
     5521 ttaaaagcca gtcgctcggc aaattatcag cgcaaaaaac aaggttttta tgcgcgcttt
     5581 tttctgacta actctgcacc aatgttgttg attgacccct cacgagatgg ccaaatcgtc
     5641 gatgctaacc tcgccgcgct caatttctac ggttataacc atgaaacgat gtgccaaaaa
     5701 catacctggg aaattaatat gctcgggcgt cgcgtcatgc ctatcatgca tgaaatttcg
     5761 catttacccg gcggtcataa acctttgaat tttgttcata aactggcgga tggatctact
     5821 cgtcatgtgc agacctatgc cgggccgatt gaaatttatg gcgacaagct catgttatgt
     5881 attgtgcatg atattactga gcaaaaacgg ctggaggagc agctggaaca tgctgctcac
     5941 catgacgcga tgaccggatt actgaatcgg cgacagtttt atcacattac ggaaccaggc
     6001 caaatgcagc acctcgccat cgcccaggat tacagcttgt tgctcatcga caccgatcgt
     6061 tttaaacaca ttaacgatct ctatgggcat tctaaaggtg atgaggtgtt atgcgccctc
     6121 gcccgcaccc tcgaaagttg cgctcgcaaa ggcgatttgg tgtttcgttg gggaggcgaa
     6181 gagtttgtct tattgctacc aagaacccca ctggataccg cgctttcgct ggctgaaact
     6241 atccgtgtaa gcgtggccaa agtgagtatt tcgagcttac cacgttttac cgtcagcatt
     6301 ggtgtggcgc atcacgaagg aaatgaaagc atcgatgaac tgtttaaacg cgttgatgat
     6361 gctttgtatc gggcgaaaaa tgatggacgc aaccgcgtgc tggcggcata agccgcggat
     6421 gcgtctcgag atcaacggct gcgcttagcg tggcgctccc agttttcttg cttcgcctgc
     6481 gccgttttac gtagtgcgac gtaacacgcc ccgctgccgc catgatgcgg tagcgcggtg
     6541 caatatgcct gaacatcatc aaattcggtc agccagcgcg ccacatagct gcggacaata
     6601 ttggcatgcg atttatcttc ccgcccttta ccatgaataa tcagcacatt acgcaaacca
     6661 tccgccaggg cttggtgaat gaaactgaac accattttgc ggcactcttc caccggctgg
     6721 cgcaaaagat tcaggctcgc ctgttgcgga tatttaccac tgcgcagctt atccagcacc
     6781 ccatgttgca acccttcccg ccgaaactcc agcggctgac ttagcgggat gatgtcgaga
     6841 aatccggtgg tgaggaaatt atcaagctgc agcgtgtcga tacgctgcgg cgcacgttgg
     6901 ttacgcgttg gatgccagtg gacatctgta gcacgtttca gcggctggac atcttccatg
     6961 gcgtcaagaa acagcgattt gtcgtcaagg ttcatgttac atcctcccgc aattaagagc
     7021 gcgatatgat aaccagaccg ggtcggtcca acaacgtatt accaaaaatt ccagtaataa
     7081 gttccaaata ttgccgatat tttaagcaaa atgcttatgc atgattattc attcacgata
     7141 ttaataatgt aacttatatt ttcgtgaaat ctgtcactga agaaaattgg caactaaagg
     7201 ttaaaaccgt tataacacag tcaccggcgc agaggagaca atgccggact taagacgcgg
     7261 atgcactgct gtgtgtactg tagagtctgg cggatgtcga cagactctat ttttttatgc
     7321 agttttaact ttgcagatag ccgcattctc gccggaattg cgtgattaat gacggggtca
     7381 gtggtgtctg gcgactatca cgccgctgaa tcaaataata ggccgctttc ggtaaaattt
     7441 cgctaaccgg caacatcacc agcccctgtc cgtgcaaggg atcgcagccc atttcttcag
     7501 gcagtatgct gagaaaatcg ctttttgcca ccagactgat acaggctgag aacgtctcgc
     7561 agactacacc gacctgtggc gtttgcgcct gatcgtcaag caattcactc aactgtttgt
     7621 agtaactgcc gtgtggcgtc ggcattgtcc agctgtaatc cagtaactgt ttgatcgaac
     7681 gggcaccaat ggcggggtgt cccgggcggc aaaagatcgc gaattgcttt tccagtaatt
     7741 tctcaaaagt aaattcgtgg tcgtacggtc cctgataata ggtattgatg gtgaaatcca
     7801 attctccctg acgcaattca ttaatcatcg acaccagttg cccttccata atgcgtactt
     7861 ttacctgcgg atgctgctga tgaaaacgag atatgacagc tggcatcaga ctgcgggaaa
     7921 tactggcccc catgccgata ttaatctgcc ctgccagttg cccttgtcgt tggcgaatat
     7981 cctcttgggc tgcgcgcagc tcttcaagaa taagactggc gtgctgataa aaactttcac
     8041 cggcatcagt taacgtcacg cctttactac ggcgaaaaaa gagttgcgcc gctaatcctt
     8101 cttccaactc ctgaatagat ttactcagtg ccggttgcga catattcaac attcggctcg
     8161 ctccgcgaat gctgccctga cgagccactt caacaaaagc acgaatttga tgaattttta
     8221 cctgaaaagc catgacgcca ccgataaccg ttatttatca gaccaaagaa actggcatct
     8281 actttaatgc agatgattgt gtcagggtaa tttatgaacg gttaaaactg tgaaaaatca
     8341 gttagtgata agtaaaaact atcgctacgt gaaccgggtc acacttttta ctgatgacgg
     8401 gaaaggttat ggagtctttg aatcaatttg ttaattcgct tgccccaaaa ttatcgcact
     8461 ggcgacgtga ttttcatcac tatgcagagt ctggctgggt ggaattccgc actgccaccc
     8521 ttgttgcgga agaattgcag cagctcggct attcactggc gctgggccgc gaagtagtta
     8581 atgaaagtag ccggatggga ctacctgatg aactcactct acaacgcgaa ttcgagcgcg
     8641 ctcgtcaaca gggtgcgcta gcacaatgga ttgcggtttt tgaaggtggt ttcactggta
     8701 tcgtcgccac cctggatacc ggtcgccccg gtccggtgat ggctttccgt gtcgatatgg
     8761 acgcgctgga tctcagtgaa gagcaggatg tcagccatcg cccctaccgc gacggttttg
     8821 cgtcatgtaa cgccggaatg atgcatgcct gtggtcatga tggacatacc gccattgggc
     8881 ttgggctagc gcataccctt aaacaattcg agtccggact acatggcgtc atcaaactga
     8941 tttttcagcc tgcagaggaa ggtacgcgtg gcgcgcgggc gatggtcgat gcaggtgtcg
     9001 tagatgatgt tgattatttt actgccgtgc acattggcac tggcgtacct gcgggcaccg
     9061 tggtgtgcgg cagtgataat tttatggcaa ccaccaaatt tgacgcgcac ttcaccggta
     9121 ccgccgctca cgcaggcgca aaaccagaag acggtcacaa tgccttgttg gcggcagcac
     9181 aagccactct tgcactgcat gcaatcgccc cggagcttcc agagtaaacg tgggcgttat
     9241 gcaggcagga agcggtcgta acgttgttcc tgcctcggcg ttgctgaaag tggaaacacg
     9301 cggggccagc gacgtcatta atcaatatgt ttttgaccgt gcacaacaag cgattcaggg
     9361 cgcagcaacc atgtatggtg tcggcgttga aactcgtctg atgggtgcag ctaccgccag
     9421 ttctccttcg ccgcaatggg tcgcatggtt gcaaagtcag gcggctcagg tcgcgggggg
     9481 caatcaggcc attgaacgtg ttgaagcgcc tgcgggttcc gaagatgcca cattaatgat
     9541 ggcccgcgtg cagcaacatc aagggcaagc ctcctacgtg gtgtttggca cacagctggc
     9601 ggcaggtcat cacaacgaaa aattcgattt tgacgagcag gttctcgcta ttgccgtcga
     9661 aacgctggcg cgcaccgcgc tcaattttcc ctggacgcga ggtatctgat gcaggaaatc
     9721 tatcgtttta tcgacgatgc gattgaagcc gatcgccaac gttataccga tattgccgat
     9781 caaatctggg atcatccaga aacacgtttt gaagagttct ggtcagcgga gcatctggct
     9841 tcggcgctgg aatctgcagg cttcaccgtt acccgcaacg taggctatat cccaaatgcc
     9901 tttattgctt cgtttggtca aggcaaaccg gttatcgccc tgctgggaga atatgacgcc
     9961 ctggcaggtt taagtcagca agcaggttgc gcgcaaccta catccgtgac gcccggtgaa
    10021 aatggtcacg gttgcggaca caatttgctg ggaaccgccg cctttgccgc tgcaatagcc
    10081 gtcaagaaat ggctggaaca atatgggcaa ggcggcacgg tgcgctttta tggttgtcct
    10141 ggcgaagaag gcggctcggg taaaacgttc atggttcgcg agggggtatt tgatgatgtg
    10201 gatgcggcac tcacttggca cccggaagcc tttgccggta tgttcaatac ccgcacgctg
    10261 gcaaacattc aggcatcatg gcgctttaaa gggatcgcag cacatgccgc gaatttccct
    10321 catttgggac gcagcgccct tgatgccgta acgttgatga ccactggcac caacttcctc
    10381 aacgaacata ttattgaaaa agcgcgcgta cactatgcca tcacaaatag cggcgggatc
    10441 tcgcccaacg tggtccaggc gcaggcagaa gtgctttatc ttatccgcgc ccccgaaatg
    10501 accgacgtgc agcatattta tgatcgggtc gccaaaatcg ccgaaggtgc ggcattgatg
    10561 accgaaacca cggttgaatg ccgcttcgac aaagcctgtt ccagttatct cccgaatcgc
    10621 accttagaaa atgccatgta ccatgcccta tcccattttg gtaccccgga atggaactcc
    10681 gaagaactgg cttttacgaa acaaattcag gctacgctcc cccccaacga tcggcaaaac
    10741 agtctgaata atatcgccgc aaccggtggc gaaaacggca aggcttttgc actacgtcat
    10801 cgtgaaacgg tactggcgaa tgaggtcgct ccatatgccg ccaccgataa cgtgcttgcg
    10861 gcatcgactg atgtcggcga cgtcagttgg aaactgcctg ttgcccagtg tttcagcccc
    10921 tgttttgccg tcggtacacc gctacatacg tggcaactgg ttagccaggg gcgaacatct
    10981 attgctcata aaggaatgct gctggcggcg aaaactatgg cagcaaccac actcaatctc
    11041 ttcattgatt cagggctatt gcaagaatgc caacaagagc atcagcaagt tacggacacg
    11101 caaccgtatc actgccctat cccaaaaaac gtgacactgt catctttaaa ataacaacaa
    11161 caacgcaaac acaacaaccg aggaatgccc atgagtatgt catccatacc gtcgtcctcc
    11221 taatccggga agctctatgg ctgggtcgaa agaattggta acaaggttcc ccatcctttt
    11281 ttgctcttta tctatttgat tatcgtactc atggtgacga cggcaatttt gtcggccttt
    11341 ggcgtcagtg cgaaaaaccc gaccgatgga acgccggtgg tggtgaaaaa cctgctcagt
    11401 gtggaaggat tacactggtt tttacccaat gttattaaaa actttagcgg ttttgctcca
    11461 cttggtgcga tcctggcgct ggttttaggt gccggtctgg cggagcgcgt cggcttactg
    11521 ccagcgctaa tggttaaaat ggcatcgcat gttaatgtcc gctacgccag ttatatggtg
    11581 ctgtttattg cttttttcag ccacatttct tccgatgcgg cgttagtgat catgccaccg
    11641 atgggtgcgc tgatttttct ggcggtgggc aggcatccag ttgcaggttt actggctgcc
    11701 attgcaggcg taggttgcgg ctttacggct aatttactga ttgtcacaac cgacgtgttg
    11761 ctgtcgggga tccgcacgga agcggcggct gcgttcaatc cgcaaatgca cgtcagtgta
    11821 attgataact ggtattttat ggccagctcc gtagtcgtac tgacgattgt tggcggcctg
    11881 ataaccgaca aaatcatcga gccacggtta ggtcaatggc agggaaacag cgatgagaaa
    11941 ctgcagacat tgaccgaaag tcagcgtttt ggtttacgca tagcaggtgt cgtatcgcta
    12001 ctttttattg ctgcgatcgc gctgatggtg atcccggaaa acgggatatt gcgcgatccg
    12061 attaatcaca ccgtgatgcc atcacccttt attaaaggta tcgtgccact aatcattctt
    12121 tttttctttg tggtttcgct ggcttatggc atcgctaccc gcacaattcg acatcaggcg
    12181 gatttaccgc atttaatgat tgaaccgatg aaagagatgg cgggatttat cgtgatggtt
    12241 tttcccctcg cccagtttgt cgccatgttt aactggagca acatggggaa attcatcgcc
    12301 gtggggctga ccgatatact ggaaagttca gggcttagcg gcatcccggc gtttgtcggt
    12361 ctggcgttgc tttcctcttt cttatgcatg tttattgcca gtggttccgc aatctggtcg
    12421 attctggccc ccattttcgt accaatgttt atgctacttg gctttcaccc ggcatttgcg
    12481 caaatcctct ttcgtattgc cgactcatcc gtattgcctt tagcgccggt atctcctttt
    12541 gttccactgt ttcttggatt cctgcaacgc tacaaaccag acgcgaaact gggtacttac
    12601 tattcgttag tcttgcccta tccgcttatc tttttggtgg tatggctgct gatgttgctg
    12661 gcgtggtatc ttgttggtct gccgataggt ccggggattt acccacgttt gtcttaagag
    12721 agaacggatg ctgagattac ttgaagaaaa aattgccacg ccactgggtc cactgtgggt
    12781 gatttgcgat gagcaatttc gcctgcgggc ggttgaatgg gaagagtaca gcgaacgcat
    12841 ggtgcagctg ctggacatcc attatcgcaa agaaggctat gagcgcattt ctgccaccaa
    12901 cccaggcggt ttaagcgaca agcttagtga ttattttgcc ggtaatctta gcattattga
    12961 tacgcttccc actgccacag gggggacgcc atttcagcgc gaagtctgga aaactctacg
    13021 cactatcccc tgcgggcagg taatgcatta cggccaactg gctgaacaat tgggccgccc
    13081 tggcgcggcg cgtgccgttg gtgcggcaaa cggatcgaat cccatcagca tcgtcgtacc
    13141 ttgccatcgg gttattggcc gaaacggcac catgaccgga tatgcaggcg gagttcagcg
    13201 aaaagagtgg ttattgcgcc acgaaggtta tcttttgctg taaacattaa acaatttgtg
    13261 ccagcttgtt cacactttta tgtaaagtta cccttaacaa cttaatggtt ttcaaataga
    13321 tagacatata tttacatcta atatcggaat tctctgctgt taaggtttgc ttagacttac
    13381 ttgctcccta aaaagatgtt aaaattgaca aatatcaatt acggcttgag cagacctatg
    13441 atcccggaaa agcgaattat acggcgcatt cagtctggcg gttgtgctat ccattgccag
    13501 gattgcagca tcagccagct ttgcatcccg ttcacactca acgaacatga gcttgatcag
    13561 cttgataata tcattgagcg gaagaagcct attcagaaag gccagacgct gtttaaggct
    13621 ggtgatgaac ttaaatcgct ttatgccatc cgctccggta cgattaaaag ttataccatc
    13681 actgagcaag gcgacgagca aatcactggt ttccatttag ctggcgacct ggtgggattt
    13741 gacgccatcg gcagcggcca tcacccgagc ttcgctcagg cgctggaaac ctcgatggta
    13801 tgtgaaatcc cgttcgaaac gctggacgat ttgtccggta aaatgccgaa tctgcgtcag
    13861 cagatgatgc gtctgatgag cggtgaaatc aaaggcgatc aggacatgat cctgctgttg
    13921 tcgaagaaaa atgccgagga acgtctggct gcattcatct acaacctgtc ccgtcgtttt
    13981 gcccaacgcg gcttctcccc tcgtgaattc cgcctgacga tgactcgtgg cgatatcggt
    14041 aactatctgg gcctgacggt agaaaccatc agccgtctgc taggtcgctt ccagaaaagc
    14101 ggtatgctgg cagtcaaagg taaatacatc accatcgaaa ataacgatgc gctggcccag
    14161 cttgctggtc atacgcgtaa tgttgcctga tttttccgca taactcacta tccttctgtc
    14221 atatcattaa atttttctga tttattgatc tggcagaagg ttcatcactg tttcattcac
    14281 cagatatggg ttaatctttt aattacaaac tgcgttgaca gttgttgtaa ggagaccctg
    14341 tatggctatg tatcagaaca tgctcgttgt tatcgatcct aaccaggacg accaaccagc
    14401 attgcggcga gctgtttatt tacatcaacg gattggtggc aaaattaaag cctttttgcc
    14461 gatctatgac ttctcatacg aaatgaccac cctgctctcc ccggacgaac gtaccgctat
    14521 gcgtcagggc gtcatcagcc agcgtacagc ctggatccac gagcaggcaa aatattatct
    14581 caatgctggc gttcccattg aaattaaagt ggtctggcat aaccgtcctt tcgaagccat
    14641 cattcaggaa gtgatcagcg gcgggcacga tttggtgcta aaaatggcgc accaacatga
    14701 ccgtctggaa gcggtgattt ttacgccaac ggactggcat ctgttacgca aatgcccaag
    14761 cccggtgtgg atggtgaaag accagccgtg gccggaagga ggtaaggcgc tggtggcggt
    14821 gaatctcgcc agtgaagagc cgtatcataa tgcgctcaat gaaaaactgg tcaaagagac
    14881 gatcgaactg gcagaacaag tcaaccatac cgaagttcat ctggttggcg cttacccagt
    14941 aacgccaatt aatatcgcga ttgaactgcc ggaatttgag ccgagcgttt ataacgatgc
    15001 cattcgtggg caacatttgc tggcaatgaa agccctgcgg cagaaattcg gcattaatga
    15061 aaacatgacg cacgtagaaa aaggtctgcc cgaagaggtt attcctgatt tggcggagca
    15121 cttacaggcg gggattgtgg ttctgggcac ggtaggacgc accggtattt cagcagcatt
    15181 cctcggcaac acggcggaac aggtgattga tcatcttcgc tgcgatctgt tggtcattaa
    15241 acctgaccag tatcagacac ccgttgaact ggatgacgaa gaagacgatt aacgactctt
    15301 caataactga caaggcccga gttatcgggc ctatttttta cttactgctc ttcgccgcca
    15361 aggaaataaa ttcccaacgg gattgccagc aacacagtaa agaccaggct ataaacaaaa
    15421 atcatcgatg actgaatcac atacattgat gtcgtccact cagacagcgg aatattgtac
    15481 tgttctataa cgccgccgat agtcgcgcga gtcacaaccg acgccgcaat aatgacaaac
    15541 cgccaacaaa ccaaagagaa atttcttccc tttcgctgac ttcagtttcg ccataatcat
    15601 gatgtaatcc ttgtgcagac aaatttcttg ttacctaaac gtaacaatac catgacattt
    15661 caccgttgtc tgctctgaaa acctcacctc ttagctggat ttacaatcaa cgcattgatt
    15721 agttag
//
LOCUS       DAJVUJ010000097        15313 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.097, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000097 DAJVUJ010000000
VERSION     DAJVUJ010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15313
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.097"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(21..464)
                     /locus_tag="OQQ48_002699"
     CDS             complement(21..464)
                     /locus_tag="OQQ48_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HCS1953410.1"
                     /translation="MAELQKVDDWLSALLANLEPAARSRMMRQLAQELRRTQQQNIRM
                     QRNPDGSSYEPRRVTARSKKGCIKRQMFTKLRTTKYLKTAASADSASVQFEGRVQRIA
                     RIHHYGLSDRVSRKGIYTIYAKRQLLGFNVQSIELIERMFIKNLT"
     gene            complement(457..888)
                     /locus_tag="OQQ48_002700"
     CDS             complement(457..888)
                     /locus_tag="OQQ48_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1953411.1"
                     /translation="MNKPQSLRHALNKAVPYVRNNPDKLHLFVDNGSLVASGASSMSW
                     EYRYTLNVVIEDFSGDQNLLMAPVLLWLRDNQPDAINNPALREKLFTFEVDILRNDVC
                     DISLNLQLTERVLVSTDGSVSNVEAVAEPDEPEEMWTVKRG"
     gene            complement(984..1412)
                     /locus_tag="OQQ48_002701"
     CDS             complement(984..1412)
                     /locus_tag="OQQ48_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysB family phage lysis regulatory protein"
                     /protein_id="HCS1953412.1"
                     /translation="MNRLLLVVLALLLAALGWQTWRLADASQTISTQADELQSKSQAL
                     AKSNSQLISLSILTETNNREQARLYAEAEQTSVLLRQRQRRIEELKRENEDLRRWADT
                     PLPADIIRLRERPALTGGAAYRQWLSASDAVSAGAGSAAH"
     gene            complement(1409..1786)
                     /locus_tag="OQQ48_002702"
     CDS             complement(1409..1786)
                     /locus_tag="OQQ48_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1953413.1"
                     /translation="MKKKLISGLFLMLWMALLIAAMVYPQGIFPVLAASGVWVACLLT
                     WAVIPVALASLIKNGPLWQELRASLLKTITRKENVFISWVIRLLIVVSLAWTGWAITL
                     VFYLLTVIAFWITHNQMAQQVAA"
     gene            complement(1788..2300)
                     /locus_tag="OQQ48_002703"
     CDS             complement(1788..2300)
                     /locus_tag="OQQ48_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCS1953414.1"
                     /translation="MNPSIVKRCLVGAVLAIAATLPGFQQLHTSVEGLKLIADYEGCR
                     LQPYQCSAGVWTDGIGNTSGVIPGKTITERQAAEGLISNVLRVERALEKCVKQQPPQK
                     VYDAAVSFAFNVGTGNACSSTLVKLLNQRRWADACRQLPRWVYVKGVFNQGLDNRRAR
                     EMAWCLQGAN"
     gene            complement(2281..2496)
                     /locus_tag="OQQ48_002704"
     CDS             complement(2281..2496)
                     /locus_tag="OQQ48_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1953415.1"
                     /translation="MTLERISAFITYCIAVVLAWLGDLSIKDASTLGGLMIGVLMLAI
                     NWYYKHKAYQLLRDGQISREDYESINR"
     gene            complement(2500..2703)
                     /locus_tag="OQQ48_002705"
     CDS             complement(2500..2703)
                     /locus_tag="OQQ48_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HCS1953416.1"
                     /translation="MKVRAHQYDTVDALCWRHYGRTQGVTEQVLKANPGLAEYGPFLP
                     HGLQVELPDIPTTTTVQTVQLWD"
     gene            complement(2703..3167)
                     /locus_tag="OQQ48_002706"
     CDS             complement(2703..3167)
                     /locus_tag="OQQ48_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HCS1953417.1"
                     /translation="MKFVAPEQAPEQAEIIRNTPFWPDVDLSEFRSVMRTDGTVTQPR
                     LKQVALSAISEVNAELYEFRRRQQMLGYASLAEVPAEQLDGKSERIQHYFNAVYCWAR
                     AMLNERFQDYDATASGAKRGEELAEASGDLWRDARWAISRVQDAPHCTVELI"
     gene            complement(3263..3913)
                     /locus_tag="OQQ48_002707"
     CDS             complement(3263..3913)
                     /locus_tag="OQQ48_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase endonuclease subunit"
                     /protein_id="HCS1953418.1"
                     /translation="MSLSPARQHRLRVQAEQAVREGGSVRHASGYDLMLLQLAEDRRR
                     LKGVQSTVKKAEIKVELLPKYAAWAEGVLAAGGAQQDDVLMYVMLWRIDAGDYAGALE
                     IGRHALRHGWVMPLGNRNVQTVLTEEMADAAQSAMLAATGFDADLLLQTLELTDGLDM
                     PDQSRARLHKAIGAVLSESNPASALNHLNHALQLDPRCGVKKDKQQLERRLRNDSR"
     gene            complement(3917..4975)
                     /locus_tag="OQQ48_002708"
     CDS             complement(3917..4975)
                     /locus_tag="OQQ48_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HCS1953419.1"
                     /translation="MKKNTRFAFNAYLQQLARLNGVAVEELSSKFTVEPSVQQTLEDQ
                     IQQSAAFLTLINVTPVTEQSGQLLGLGVGSTIAGTTDTTAKEREPVDPTLMVDVEYKC
                     EQTNFDTVLTYAKLDLWAKFQDFQVRIRDAIVKRQALDRIMIGFNGVKRAKTSNRSEN
                     PLLQDVNKGWLQKIREDAPDHVMGSTTTGGETTPGAVKVGKGGEYANLDAVVMDAVNE
                     LIDVVYQDDDDLVVICGRELLSDKYFPLVNKEQENSEKLAADMIISQKRMGGLQAVRA
                     PFFPPNALLITRLDNLSIYWQEDTRRRSVIDNPKRDRIENFESVNEAYVVEDYRCAAL
                     VENIQISDFSAAAAETGA"
     gene            complement(4992..5825)
                     /locus_tag="OQQ48_002709"
     CDS             complement(4992..5825)
                     /locus_tag="OQQ48_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HCS1953420.1"
                     /translation="MTVKAKRFRIGVEGATTDGREIQREWLEQMAASYNPAVYTALIN
                     LEHIKSYLPDSTFNRYGKVTALFAEEITEGPLAGKMALYADVEPTESLVELVKKGQKL
                     FTFMEVSPKFADTGKAYLVGLAATDDPASLGTEMLTFSASAAHNPLANRKQNPANLFT
                     AAEETVIELEEVQDDKPSLFARVTALFTKKEQSDDARFSDVHKAVELVATEQQNLSAR
                     TEKSLSEQEERLSELETALQAQQTAFNELVDKLSHEDSRQDYRQRATGGNAPADTLTN
                     C"
     gene            5968..7734
                     /locus_tag="OQQ48_002710"
     CDS             5968..7734
                     /locus_tag="OQQ48_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase ATPase subunit family protein"
                     /protein_id="HCS1953421.1"
                     /translation="MNTTLTPADLDPRRQAMLLYFQGYRVARIAEMLGEKVATVHSWK
                     KRDKWGDYGPLDQMQLTTAARYCQLIMKEHKEGKDFKEIDLLARQSERHARIGKFNNG
                     GNEADLNPNVANRNKGPRRQPEKNVFTDDQIEKLEKIFHSSMFNYQRHWWEAGKTNRI
                     RNLLKSRQIGATFYFAREALIDALLTGRNQIFLSASKAQAHVFKQYIIDFAKEVEVEL
                     KGDPMVLPNGATLYFLGTNARTAQSYHGNLYLDEYFWIPKFQELRKVASGMAIHKKWR
                     QTYFSTPSSLTHSAYPFWSGALFNRGRNKADKVDIDLSHSNLAPGRLCADGQYRQIVT
                     VEDAVRGACNLFDLDQLRMEYSPDEYQNLLMCEFVDDLASVFPLSELQACMVDSWEVW
                     TDFHALALRPFGWREVWIGYDPAKGTQNGDSAGCVVVAPPAVPGGKFRILERHQWRGM
                     DFRAQADAIKKLTEQYNVTYIGIDSTGVGHGVYENVKAFFPAVREFVYNPNVKNALVL
                     KAYDIISHRRLEFDAGHTDIAQSFMAIRRATTASGNRPTYEASRSEEASHADLAWATM
                     HALFNEPLQGESANTSNIVEIF"
     gene            7734..8330
                     /locus_tag="OQQ48_002711"
     CDS             7734..8330
                     /locus_tag="OQQ48_002711"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1953422.1"
                     /translation="MGKSKKNRAAATKQTQHKNQTSAEAFSFGDPVPVLDRRELLDYV
                     ECVQTDRWYEPPVSFDGLARTFRAAVHHSSPIAVKCNILISTYIPHPLLSQQAFSRFV
                     QDYLVFGNAYLEKRTNRFGEVIALEPALAKYTRRGLDLDTYWFVQYGMTTQPYQFTKG
                     SIFHLMEPDINQEIYGLPGYLSAIPSALLNESATLFRR"
     gene            8364..8765
                     /locus_tag="OQQ48_002712"
     CDS             8364..8765
                     /locus_tag="OQQ48_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCS1953423.1"
                     /translation="MYMTDAAQNQEDVNNLRNAMKSAKGPGNFRNLFMYSPNGKKDGL
                     QIIPLSEVAAKDEFLNIKNVSRDDMMAAHRVPPQMMGIMPNNVGGFGDVEKASLEFVR
                     NELIPLQKRMMEINKLVSENIVKFDSYKLAT"
     gene            complement(8872..9753)
                     /locus_tag="OQQ48_002713"
     CDS             complement(8872..9753)
                     /locus_tag="OQQ48_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004982020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1953424.1"
                     /translation="MKWAIEMNKLKRPVFKDYSAFKKLSANKRLKHHSVLNGQDTFIL
                     NAYRSYIKNKGYLNVNSCGVLSNNIAEALRYYYNNPAKCLSIIDDIRAANSNSLCPMC
                     GSMHSGTLDHVLPKENYPEFSLFTKNLVPACKCNTFKSTTIANSAGHRMLHPYFDNIL
                     KQRLICANFSKLGRTPTIDVKIIIDPMSPEYNNVKYHLDNVVIKNNIKGYLSEQWESF
                     YLYPELVIRGLNKSLSTYSDVYELLTRELLLLDEKHKGKNNWNSVFVAGLIRPDVIRW
                     ILCELHLGNRSPDGRLI"
     gene            complement(9737..11386)
                     /locus_tag="OQQ48_002714"
     CDS             complement(9737..11386)
                     /locus_tag="OQQ48_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000960487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCS1953425.1"
                     /translation="MLVKKNKNYSLYFLITNNQQISDSGNYIKIVPSRSDWNDFGYLS
                     RVEISIFNKKECKDIISLNGYIGFLNSEEERNGKAELIRRVKKKEESNLKDEGFNDYF
                     VMLQDMNNYRRLVRFFGVNESKYILNFINDVVSKKSDASAENLRKKAISSDIFNKSFI
                     RESESYFTFKNAGTVLSGIQYEELGNLSQHIKISYSNKVNEEDVSYVFNFDHEHDLPK
                     RISIIIGENGVGKSQTLREIALAAIKGKDNLVAVVDSNGVSIEERIQISRLIAFSPTN
                     ESKCSFPTDKRIKSLIWYKRLTLNRESKSKLNLNDLIVQLARSNDAIGYNQRWNIFVD
                     ALSVLNSNNDLVIKCGGENSPFVKLNELNSRGEESRLSLYSEIILSGEPLRLIENKCY
                     PLSSGEISFIKFCVQVCLYIENGTLLLIDEPETHLHPSFINKFMALLDNLLEKTGSSA
                     IIATHSVYLVREVFKEQVTILRRRDDGVIVSEKPRLSTFGANIGNISYFVFGESEPTQ
                     ILNKVKNNIIAKNMSWDDVESKYSEDLSLNVLTALRNEMGN"
     gene            complement(11679..11912)
                     /locus_tag="OQQ48_002715"
     CDS             complement(11679..11912)
                     /locus_tag="OQQ48_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI family protein"
                     /protein_id="HCS1953426.1"
                     /translation="MRIEIMIDKEQKISQSTLDALESELYRNLRPLYPKTVIRIRKGS
                     SNGVELTGLQLDEERKQVMKIMQKVWEDDSWLH"
     gene            complement(11923..12111)
                     /locus_tag="OQQ48_002716"
     CDS             complement(11923..12111)
                     /locus_tag="OQQ48_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1953427.1"
                     /translation="MQDYFLESLKLQRIDFFLKLVAASECSDEEKGLALQWVSELTDE
                     LMAKIRTHEYNRSMDVIS"
     gene            complement(12265..14679)
                     /locus_tag="OQQ48_002717"
     CDS             complement(12265..14679)
                     /locus_tag="OQQ48_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HCS1953428.1"
                     /translation="MSHADMNNCCGFNEAAAAFSWNSPKKAINPYLDPAEVAPVSALS
                     NLITLYAADNEQEQLRREALSDQVWERYFFYESRDPVQHEMEQDKLISRAKLAHEQQR
                     FNPDMVILADVNAQPSHISKPLMQRIEYFSSLGRPKAYSRYLRETIKPCLERLEHVRD
                     SQLSASFRFMASHVGLDGLLILPEMSQDQVKRLSTLVAAHMSMCLDAACGDLYATDDV
                     KPEEIRKTWEKVAAETLRLDVIPPAFEQLRRKRNRRKPVPYELIPGSLARMLCADWWY
                     RKLWKMRCEWREEQLRAVCLVSKKASPYVSYEAVMHKREQRRKSLEFFRSHELVNEEG
                     DTLDMEDVVNASSSNPAHRRNEMMACVKGLELIAEMRGDCAVFYTITCPSRFHSTLNN
                     GRPNPTWTNATVRQSSDYLVGMFAAFRKAMHKAGLRWYGVRVAEPHHDGTVHWHLLCF
                     MRKKDRRAITALLRKFAIREDREELGNNTGPRFKSELINPRKGTPTSYIAKYISKNID
                     GRGLAGEISKETGKSLRDNAEYVNPWASLHRVQQFRFFGIPGRQAYRELRLLAGQAAR
                     QQGDKKAGAPVLDNPRLDAILAAADAGCFATYIMRQGGVLVPRKYHLIRTAYEINEEP
                     TAYGDHGIRIYGIWSPIAEGKICTHAVKWKMVRKAVDVQEAAADQGACAPWTRGNNCL
                     LAENLNQQEKDKSADGNPRTDITSMDDKELHDYLHSMSKKERRELAARLRLVKPKRRK
                     DYKQRITDHQRLQLVYELKSRGFDGSEKEVDLLLRGGSIPSGAGLRIFYRNQRLQEDD
                     QWRNLY"
     gene            complement(14676..>15313)
                     /locus_tag="OQQ48_002718"
     CDS             complement(14676..>15313)
                     /locus_tag="OQQ48_002718"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HCS1953429.1"
                     /translation="RVLFEIANREVAYYNIRQEFNYSTEITDFMKAVYFLYLNRHGYR
                     GLCRYNKSGHFNIPYGNYKNPYFPEKEIRAFAEKAQRATFICASFDETLAMLKAGDVV
                     YCDPPYDGTFSGYHTDGFTEDDQYHLASVLEHRSSEGHPVIVSNSDTSLIRSLYRNFT
                     HHYIKVKRSIGVAAGEGKSATEIIAVSGPRCWVGFDYSRGVDSSAVYGVRA"
BASE COUNT         3741 a         4138 c         3524 g         3910 t
ORIGIN      
        1 caattgaaaa caaataaaaa tcaagttagg tttttaatga acattctttc aattaactca
       61 atagattgga cattaaaacc taataactgc cttttcgcat agatggtata aatacctttt
      121 cgactgacgc gatcactcaa gccgtaatgg tgaatacggg caatgcgctg caccctacct
      181 tcaaactgta cgctggcaga atcggcgctg gctgcagttt tcaggtattt tgtggtgcgc
      241 agctttgtaa acatctgacg tttgatgcac ccctttttgc tgcgtgctgt taccctgcgc
      301 ggctcataac tgctgccatc aggattgcgc tgcatcctga tgttctgctg ctgtgtccgg
      361 cgcagttcct gcgccagctg gcgcatcatg cggcttcttg cggctggctc cagattcgcc
      421 aataatgcac tcagccagtc gtccaccttc tgcaattcag ccacgtttca ccgtccacat
      481 ttcttcaggt tcatcaggtt ccgctaccgc ttcaacgttc gacacactgc cgtcagtgct
      541 gaccagcaca cgctccgtca gttgcaggtt aaggctgata tcacagacat cgttgcgcag
      601 aatatccacc tcaaaggtga atagcttttc ccgtaacgcc gggttattga tcgcatcggg
      661 ctggttatcc cgcagccaca gcaaaaccgg ggccatcagc agattctggt cgccgctgaa
      721 atcttcaatc accacgttca gggtgtagcg gtactcccat gacatggagc tagccccgga
      781 ggcaaccagc gaaccgttat ccacaaacag atgcagtttg tccgggttat tacggacata
      841 aggcactgct ttattgaggg cgtggcgcag ggattgtggt ttgttcactg tttcgctcct
      901 gacacgcaat aatcatgtcc actttgtctg cacagaccgc ccaggcggcc tccgtttcat
      961 ccagcaacgc attcagatca ccgttagtgc gcggcgctgc ctgctccagc cgacacggcg
     1021 tcactcgcgg acaaccactg acggtaagct gcacctccgg tgagtgccgg acgttcccgc
     1081 agccggataa tgtcagcagg caaaggagta tcagcccagc gacgtaaatc ctcgttctca
     1141 cgtttcagtt cctcaatccg gcgttgtcgt tgtctcagca gtacactggt ctgttctgct
     1201 tcggcataga gccgcgcctg ctcccggtta ttggtttcag tcagaatgga caggctgata
     1261 agctggctgt tgctctttgc cagtgcctgg cttttgctct gcagctcgtc tgcctgcgtg
     1321 ctgatggtct ggctggcatc agccagccgc cacgtctgcc agcccagcgc cgccaataat
     1381 aacgccagca caaccagcag caaccggttc atgcggctac ctgttgcgcc atctgattat
     1441 gggtgatcca gaaggcaata acggtcagca gataaaagac cagggtaatg gcccaccccg
     1501 tccaggcgag actgacgaca atcagcaatc gtatcaccca gctgataaat acgttttctt
     1561 ttcgggtaat tgtcttcagt aaagatgccc tcaactcctg ccagagcggg ccattcttaa
     1621 ttaacgaagc cagtgctacc ggaattaccg cccatgtcag taaacaggct acccaaacgc
     1681 cggacgctgc cagtaccgga aaaatcccct gcggatacac cattgctgcg attaacagcg
     1741 ccatccataa catcagaaac agcccgctga ttaatttctt tttcatttca gtttgctccc
     1801 tgtaaacacc aggccatctc ccgcgcacgg cggttatcca gcccctgatt aaaaacacct
     1861 ttcacataaa cccagcgcgg caactgtcgg cacgcatccg cccagcgccg ctgattgagc
     1921 aatttcacca gcgtggaact gcaggcattg cccgtaccca cgttgaaggc aaacgacacc
     1981 gcagcgtcat acaccttctg cggcggctgt tgcttcacac acttttccag cgcccgctcc
     2041 acacgcagca cgttggagat cagcccttct gctgcctgtc gctccgtaat ggttttgccg
     2101 ggaatgacgc ccgacgtatt accaataccg tcggtccaga cacccgcgct gcactgatac
     2161 ggctgcagac gacaaccttc gtaatcggca atcagtttca gtccctccac ggaggtgtga
     2221 agctgctgaa aacccggcag cgtggcagca atagccagca cggccccgac aaggcagcgt
     2281 ttaacgattg atggattcat agtcctcccg cgagatctgc ccgtcgcgca gaagctggta
     2341 ggctttgtgt ttgtagtacc agttgatagc cagcatcagc acaccaatca tcaggccgcc
     2401 cagcgttgag gcatccttga tggacaaatc gcccagccag gccagcacga cggcgatgca
     2461 atacgtgata aaggcgctga ttcgctcaag cgtcataatt cagtcccata gctggacggt
     2521 ctgcacggtg gtggtggtcg gaatgtccgg cagctccacc tgcagcccgt gaggtaaaaa
     2581 ggggccgtat tcggcaagcc ccggatttgc cttcagtacc tgctccgtga caccctgcgt
     2641 gcgcccgtaa tgacgccagc aaagcgcgtc caccgtgtca tactgatgcg cacgcacttt
     2701 catcagataa gctccactgt gcagtgcggc gcgtcctgca cccggctgat ggcccagcgg
     2761 gcgtcacgcc acaaatcacc gcttgcttcc gccagttctt cgccccgctt cgcaccggat
     2821 gccgtggcgt catagtcctg gaaacgttcg ttgagcatgg cgcgagccca gcagtaaacc
     2881 gcgttgaaat agtgctgaat gcgctcactt ttcccgtcca gctgttccgc cggaacctca
     2941 gccagcgacg cataccccag catctgctgg cgtctgcgaa actcatacag ctctgcgtta
     3001 acctccgaaa ttgccgacag tgcaacctgc tttaaacgcg gctgcgtcac cgtgccgtca
     3061 gtgcgcatga cactgcgaaa ctccgacagg tccacatcag gccagaacgg cgtatttcta
     3121 atgatttccg cctgttccgg tgcctgttct ggcgcaacaa acttcatgct gctttctcct
     3181 gaaataaagg gcggtggacg gggttttgat gtggctgtgc ctttcgccac cccgtgccgc
     3241 ccgtgcgcgg gggcacgttc tgtcagcggc tgtcattgcg cagtctgcgc tccagctgct
     3301 gtttgtcttt tttcacgcca cagcggggat cgagctgtaa cgcatggttg agatgattaa
     3361 gggcggaagc cggattgctt tcactcagga ccgcgccaat cgctttatgc agacgtgccc
     3421 gtgactggtc cggcatatcc agaccgtctg tcagctccag cgtctgcagc aacagatcgg
     3481 catcaaagcc ggtggcggca agcattgcgc tctgcgctgc atctgccatt tcctctgtca
     3541 gcacggtctg cacgttgcgg ttgcccagcg gcatcaccca tccatgacgc agggcatgac
     3601 gcccgatctc cagcgccccg gcataatctc cggcatcaat gcgccacaac atcacgtaca
     3661 tcagcacgtc atcctgttga gcgcctccgg cagccaggac accctccgcc caggcggcgt
     3721 acttcggcag cagctccact ttgatttccg cttttttgac cgtggactga acgcccttga
     3781 gacggcggcg gtcttccgcc agttgcagca gcatcaggtc atagcccgat gcgtggcgaa
     3841 cactgccacc ctcacggacg gcctgttcag cctgaacgcg caggcgatgc tgccgtgcgg
     3901 gactcaggct catggattac gctccggttt cggctgcggc ggcgctgaaa tcactaatct
     3961 ggatgttttc caccagtgcg gcgcagcggt agtcctcaac cacataggct tcgttaacgg
     4021 attcaaagtt ttcaatccgg tcacgtttcg ggttgtcgat aactgaacgg cggcgggtgt
     4081 cttcctgcca gtagatggac aggttatcca gacgggtgat cagcagcgca ttcggcggga
     4141 agaacggcgc acgcacggcc tgcaggccac ccatgcgttt ctgactgatg atcatatcgg
     4201 cagccagttt ttcactgttt tcctgctctt tgttgaccag cgggaaatac ttgtcagaca
     4261 gcagttcacg accgcaaatc accaccagat cgtcatcgtc ctggtagacc acgtcgataa
     4321 gctcattgac ggcatccatc accacggcgt ccaggttggc atattcgcca cctttcccga
     4381 ctttcactgc gcccggtgtg gtttcaccgc ccgtggtggt gctgcccatg acgtgatccg
     4441 gtgcatcctc acggattttc tgcagccagc ctttattcac atcctgcagc agcgggtttt
     4501 cgctacggtt ggaggttttc gcacgcttca cgccattaaa gccgatcatg atgcggtcca
     4561 gtgcctgacg tttcacgatg gcgtcacgga tacgcacctg aaaatcctga aacttcgccc
     4621 acaggtccag cttcgcgtag gtcagcaccg tgtcaaagtt ggtctgctcg catttatatt
     4681 ccacatcgac catcagcgtc ggatcgacag gttcacgctc tttcgcagtg gtatcagtgg
     4741 ttccggcaat ggtgctgcca actcccaacc ccagcagctg accggactgc tcagtcactg
     4801 gcgtgacgtt aatcagagtc aggaaagcgg cggactgctg gatctggtct tccagcgtct
     4861 gctgcacgga cggctctacg gtgaacttgc tggacagttc ttcaactgcc acaccgttca
     4921 gacgcgccag ttgctgcagg taagcgttaa aagcaaagcg ggtattcttc ttcatcaggt
     4981 tttgtgctcc atcagcaatt ggtcagagtg tcagcggggg cgttaccgcc tgttgcacgc
     5041 tggcggtagt cctggcggct gtcttcatga ctcagcttgt ccaccagttc gttaaaggcg
     5101 gtttgctgtg cctgcagggc agtctccagc tcagacaggc gttcttcctg ctcagacagg
     5161 gatttttcgg tgcgtgcgct caggttctgc tgctcagtgg cgaccagctc cacggcctta
     5221 tgcacatcag agaaccgggc gtcatcggac tgctcttttt tggtaaacag cgccgtgacg
     5281 cgggcaaaca gggacggttt gtcgtcctgg acttcttcca gttcgatcac cgtttcctct
     5341 gcggcggtaa agagattggc gggattctgc ttgcggtttg ccagcgggtt atgggctgca
     5401 ctggcgctga atgtcagcat ttcagtgccc agactggcag ggtcatcagt agcagccagg
     5461 ccgactaggt aggctttgcc cgtatcagcg aacttcgggc tgacttccat aaaggtgaat
     5521 aatttctggc cttttttcac cagttccacc agggactccg ttggctcaac gtcagcatac
     5581 agcgccatct tgcctgccag cggaccttcc gtgatttctt cagcaaacag cgccgtcacc
     5641 ttgccgtagc ggttaaaggt gctgtccggc agataagact tgatgtgctc aaggttaatc
     5701 agcgcggtat acaccgccgg gttatagctg gctgccatct gttccagcca ttcacgctgg
     5761 atttcgcgtc cgtcggtggt ggcaccttcc accccgatgc gaaaacgctt tgctttcact
     5821 gtcatgagcc gtgctccgtt agaaaaaact tactggagcc ttatggttgc ggtgataggg
     5881 gcagtgaaac aatgcgcggt atttgtaccg acaaccacac aaaccgcagg cggggaaagc
     5941 cttcattcaa ggctgtaggt ttgtgccatg aacaccacac tgacacccgc agatctcgat
     6001 ccccgtcggc aggccatgct gctgtacttt cagggatacc gcgtagcccg cattgctgaa
     6061 atgctgggcg agaaagttgc aaccgttcac agctggaaaa aacgcgacaa gtggggtgac
     6121 tatgggcctc tggatcagat gcagctcacc accgccgcac gctactgcca gctcatcatg
     6181 aaggagcaca aagaagggaa agatttcaaa gagattgacc tgctggcgcg ccagtctgag
     6241 cgccacgcgc ggatcggcaa gtttaacaat ggcggcaacg aagccgactt aaaccctaac
     6301 gtcgccaacc gcaacaaagg cccacgccgt cagcctgaaa agaatgtttt caccgatgac
     6361 cagattgaga agctggaaaa aattttccat tcctccatgt tcaactacca gcgccactgg
     6421 tgggaagccg gaaaaaccaa ccgcatccgc aacctgctga agtcacgcca gatcggcgcg
     6481 accttctatt ttgcccgtga agccctgatt gacgccctgc ttaccggacg taaccagatt
     6541 ttcctttctg ccagtaaggc acaggcccac gtcttcaaac agtacatcat cgactttgcc
     6601 aaagaagtgg aggtggagct gaaaggcgat ccgatggtgc ttcccaacgg ggccacactg
     6661 tatttcctcg gcaccaatgc ccgcactgcc cagagttatc acggtaacct gtatctggat
     6721 gaatatttct ggataccgaa atttcaggag ctacgcaaag tggcttccgg tatggctatt
     6781 cacaagaaat ggcggcagac ctatttttcc acaccatcca gtctgaccca cagtgcttat
     6841 ccgttctggt ccggtgcgct gttcaaccgt gggcgcaaca aagctgacaa ggtggacatc
     6901 gacctgtccc acagcaatct ggcccccggc cggctgtgcg cagacgggca ataccgccag
     6961 atagtcaccg tggaagatgc ggtgcgcggc gcctgtaacc tgttcgacct tgaccagttg
     7021 cgcatggagt acagccctga cgaataccag aacctgctga tgtgcgagtt cgtggacgat
     7081 ctcgcgtccg tgttcccgct cagcgaattg caggcgtgca tggtggacag ctgggaagtc
     7141 tggaccgact ttcatgcact ggccctgcgc ccgtttggct ggcgcgaagt gtggatcggt
     7201 tatgacccgg caaaaggtac gcagaacggc gacagcgccg gatgcgtggt ggtggcaccg
     7261 ccagccgtgc caggcggtaa gttccgcatt cttgagcgtc accagtggcg cggaatggac
     7321 tttcgcgccc aggctgacgc catcaaaaaa ctgactgaac agtacaacgt gacctatatc
     7381 ggcatcgact caaccggcgt tggtcacggg gtttacgaga acgtgaaagc gttttttcct
     7441 gccgtccggg agtttgtcta caaccccaac gttaaaaacg ccctggtact caaggcctac
     7501 gacattatca gccaccgccg tcttgagttt gacgccgggc acaccgacat tgcgcagtca
     7561 ttcatggcaa tccgtcgcgc caccaccgcc agcggcaacc gcccgaccta tgaagccagc
     7621 cgcagcgaag aagccagcca tgccgatctg gcctgggcaa caatgcacgc actgtttaac
     7681 gaaccgctgc agggcgagtc cgccaatacc agcaatattg tggagatttt ttgatgggaa
     7741 agagtaagaa gaaccgcgct gcggcgacga aacagaccca gcataaaaac cagacttcag
     7801 ccgaagcatt cagcttcggc gatcccgttc cggttctgga ccgccgtgaa ctactggact
     7861 atgtggaatg cgtacagaca gatcgctggt atgagccgcc agtgagtttt gacggactgg
     7921 cgcgcacctt ccgcgccgcc gtgcatcaca gttcaccaat tgcggtgaaa tgcaacattc
     7981 tgatcagtac ctacatccct cacccgctgc tcagccagca ggctttttca cgttttgtgc
     8041 aggattatct ggtatttggt aacgcttacc tggagaaacg cacaaaccgg ttcggagagg
     8101 tcatcgctct tgagcctgct ctggcaaaat acacccgacg cggattagac ctggatacct
     8161 actggtttgt gcaatacggc atgaccacgc agccgtatca gttcacgaaa ggaagcatct
     8221 ttcatctgat ggaaccggac atcaaccaag agatctacgg cctgccaggt tacctttctg
     8281 ccattccgtc agccctgctc aacgagtccg ccacgctgtt ccgccgatag tattacatta
     8341 acggcagtca tgcaggcttc atcatgtaca tgaccgatgc cgcgcaaaac caggaggatg
     8401 tgaacaacct ccgcaatgcg atgaaaagcg ccaaaggacc aggtaacttc cgcaatctgt
     8461 ttatgtactc gcctaacggc aaaaaggacg ggcttcagat catcccgttg tcagaagtcg
     8521 cggcgaagga tgagtttctg aacatcaaga acgtgagccg ggatgacatg atggcggcgc
     8581 atcgtgtgcc accgcaaatg atggggatta tgcctaataa tgtcgggggg tttggggatg
     8641 tggagaaggc gagcctggag tttgtacgta acgagttaat accgttacaa aaaagaatga
     8701 tggaaataaa taaattggta agtgagaaca ttgttaaatt tgatagttat aaactagcta
     8761 cttaacgtaa tcactaaatg aggttcttta acacagctaa aaaatttatt aatagccagc
     8821 agtttttatg aatttcttca gcgtgcagat acacgctgaa gatgcaaaaa atcatatgag
     8881 acgaccgtct ggacttctgt tacctaaatg taattcacag agtatccacc taatcacatc
     8941 aggtcgaatt aacccagcta caaaaaccga attccaatta tttttacctt tatgtttttc
     9001 atccaacagc aacaactcgc gagtcaacaa ctcatataca tcgctataag tagaaagact
     9061 cttattcagt cctctaataa caagctcagg atataagtaa aacgactccc attgttcaga
     9121 caaatacccc ttaatattat tcttgataac aacattatca agatgatact taacattgtt
     9181 atattctggt gacattgggt caattatgat ttttacatca attgtaggtg ttcgccctaa
     9241 tttagaaaaa ttagcacata ttaatctttg ttttaatata ttatcaaaat atggatgcag
     9301 catccgatgg cctgcagaat tagctatagt agtacttttg aaagtattac atttgcaagc
     9361 aggaaccaaa ttcttagtga ataaagaaaa ttctggatag ttttccttgg gtaaaacatg
     9421 atccaaagtc ccgctatgca ttgaaccaca cattggacat agagagttag aattagctgc
     9481 tcttatatca tcaattatag ataaacactt tgcgggattg ttataataat atctcaaagc
     9541 ttcagcaata ttgttactca atacgccaca agaattgaca ttaagatacc ctttattctt
     9601 tatgtacgat ctatatgcat taagtataaa tgtatcctgt ccattcagaa cagaatgatg
     9661 ttttaatctc ttatttgcag atagtttttt aaatgcagaa taatctttaa aaacaggtct
     9721 ttttaattta ttcatttcaa ttgcccattt catttctcaa cgcagttaat acattaagtg
     9781 ataagtcctc gctatattta gattcaacat catcccaaga catattcttt gcaattatat
     9841 tgttttttac tttatttaaa atttgagttg gttcactctc accgaaaaca aaatatgaga
     9901 tatttcctat attcgcccca aaagtactca atctcggttt ttcgctaaca attaccccat
     9961 catctcgtct tctgagtatt gtcacttgct ccttaaaaac ctcccgaaca agataaaccg
    10021 aatgcgttgc tatgatagct gaagagcctg tcttttctaa taaattatca agcaatgcca
    10081 taaatttatt aataaatgaa ggatggagat gtgtttctgg ctcatctatt aatagaagag
    10141 tcccattctc aatatacaaa cagacttgta cacaaaactt aataaacgat atttccccac
    10201 tactcaaagg gtaacattta ttttcaataa gcctcaaagg ctcgccagag agaataattt
    10261 cagaatataa tgatagtcta gattcttcgc ctcgagaatt aagctcattt aatttaacaa
    10321 atggtgaatt ttcaccgcca catttgatta ctaaatcgtt gttactattt agaacactca
    10381 gtgcatcaac aaatatattc cacctttgat tatagccgat agcgtcgtta ctcctcgcta
    10441 gttgcactat taaatcattc aagtttaatt tggattttga ttcacgattt aaagtcaatc
    10501 ttttgtacca tatcaatgat tttattcttt tatcggttgg aaatgaacac ttactttcat
    10561 ttgttggaga aaaagcaatg agcctactaa tttgaattcg ttcttcaatg ctcacaccgt
    10621 tagaatcaac tacagccacc aaattatcct ttcctttaat cgcagccaat gcgatttctc
    10681 taagcgtttg gctttttcct acaccattct cgcctataat tatggaaatt ctttttggta
    10741 aatcatgctc gtgatcaaaa ttaaaaacat aagatacatc ttcttcatta actttattcg
    10801 aatagctaat cttgatgtgc tgtgataaat tacccaactc ctcatattga ataccggaaa
    10861 gaactgttcc tgcatttttg aaagtaaaat acgactcaga ctctctaatg aaagatttat
    10921 taaagatatc agaagagatt gcttttttcc tcaaattctc tgcactagcg tcacttttct
    10981 tagaaacaac atcattaatg aaatttaata tatatttgga ttcattcacc ccaaaaaatc
    11041 ttactagtct tcgataatta ttcatatcct gaagcatgac aaaataatca ttaaacccct
    11101 catcctttaa attagattct tcctttttct tcactcgcct tatcagttct gcttttccat
    11161 ttcgttcctc ctctgaattc aaaaaaccaa tataaccatt cagtgaaatt atatccttac
    11221 actctttttt attaaaaata gatatttcga ctctactcaa atatccaaaa tcattccaat
    11281 cgcttcttga tgggactatt tttatataat taccagaatc agatatctgt tgattatttg
    11341 taattaaaaa atataaacta taatttttat ttttcttgac taacattatt accctctcac
    11401 ttcattagtg atgatttatt ttggaattat tttacacact tatacctagc gcgcaatcgt
    11461 atccccgcca cgcctgcccg ctttatgtag aggttttcat gcacctgcat gatctacgca
    11521 aaagcccgcc agttctggcg ggccttagca aaaacgatcc tcaaacgatc atgcgatctc
    11581 atgcggcata gacatgcact acagagctaa cgcctcgcaa gggctcgttg ttcaaccttg
    11641 ctgacgccag aagctagttc agacgccagc aacgtttctt aatgcagcca gctgtcgtct
    11701 tcccacacct tctgcataat tttcatcact tgttttcttt cttcgtccag ttgcaatcca
    11761 gtaagttcca caccgttaga gctacctttg cggatacgaa ttaccgtttt gggatacaga
    11821 gggcgcagat tgcggtaaag ctcggattca agagcgtcca gggtagactg gctaatcttc
    11881 tgctctttat cgatcattat ttcaatgcgc ataaaagtca cctcagctga tgacatccat
    11941 tgagcggttg tattcgtggg ttctgatttt tgccatgagt tcatcagtca attcagaaac
    12001 ccactgcaga gccagcccct tctcttcatc actacactca ctagccgcta caagcttaag
    12061 aaaaaaatca atgcgctgga gcttcaaaga ctccaaaaaa tagtcctgca tctttcctcc
    12121 tatgacacca cacgcaatac tgtatgcata accactgttt atatttacag tatataataa
    12181 tcttactgat gtaaaacgtt tttttacgtt catcagcctg atatgcctgg tattattaag
    12241 agcacgaatt gttaacccgc gtaattaata caggttccgc cactgatcat cttcctgcaa
    12301 acgctggttc cgatagaaga tacgcaggcc tgctcctgac ggaatactgc ctccgcgaag
    12361 gagtaaatcg acctctttct cgctgccatc aaatcctctg gacttcagct catacacgag
    12421 ctgcagtcgc tgatggtctg taattcgctg tttgtagtct ttacgccgtt tcggtttcac
    12481 caggcgtaac cttgctgcca gttcccggcg ctcttttttg ctcatactgt gcaggtaatc
    12541 gtgcaactcc ttgtcatcca tgctggtaat gtccgttctg gggttcccat cagctgattt
    12601 atctttctcc tgttggttca aattttcagc aaggagacag ttattgccac gagtccaagg
    12661 ggcgcaagcg ccctggtcgg ctgccgcctc ctgaacgtca acggctttac gaaccatttt
    12721 ccacttcact gcatgagtgc agatcttgcc ttctgcaatg ggtgaccaga tgccataaat
    12781 acgaataccg tgatcgccat aggcggtcgg ctcttcattg atttcataag cggttctgat
    12841 caagtgatat ttgcgaggaa ccagcacgcc gccctgcctc ataatgtagg tggcaaaaca
    12901 gccagcatca gcagcagcca ggatggcatc aaggcgcggg ttatccagta ccggcgcacc
    12961 tgcttttttg tcaccctgct gccttgccgc ctgaccagcc aacaaccgca gttcacggta
    13021 agcctgacgc cctggaatac caaagaagcg gaattgctga acacgatgca gagacgccca
    13081 gggattaacg tattcagcgt tatcacgcag ggatttaccc gtttccttgc tgatctcgcc
    13141 agccagacca cgcccgtcaa tgttcttact gatgtatttc gcgatgtagc ttgtcggcgt
    13201 tcctttgcgc gggtttatca gctcagattt aaagcgtgga ccagtgttat tacccagttc
    13261 ctcgcggtct tcacggatgg caaacttacg caacaatgca gtaatggcgc ggcggtcttt
    13321 tttgcgcata aaacacaaca ggtgccagtg aactgtaccg tcatgatgcg gctcagccac
    13381 ccgcacgcca taccagcgca acccggcttt gtgcatcgcc ttacgaaatg cagcaaacat
    13441 gccgaccaga taatcgctgc tttgtcttac cgtcgcattt gtccaggttg ggtttggtct
    13501 gccgttattg agcgtggaat ggaaacgtga cggacaggta atggtgtaga aaacggcgca
    13561 gtcaccgcgc atttccgcga taagctccag acctttaaca caggccatca tctcattgcg
    13621 gcggtgcgcc gggttgctgc tgctggcgtt taccacgtct tccatatcca gcgtgtcgcc
    13681 ctcttcgttc accagttcat gagaacggaa aaactccaat gacttgcggc gctgctcacg
    13741 tttatgcatc acggcttcat agctgacata gggagatgct tttttgctga ccaggcagac
    13801 agcacgcaac tgctcttccc gccattcgca acgcattttc cataatttcc ggtaccacca
    13861 gtcggcgcac aacatacgcg ccagcgaacc cggaatgagt tcatagggca cgggtttacg
    13921 gcggtttctt ttccgacgga gttgctcaaa cgcaggcggg atgacatcca gacgcagggt
    13981 ttccgctgcc accttttccc atgtcttgcg gatttcttct ggcttaacgt catcggtggc
    14041 atacaaatca ccacaagctg catcaaggca catgctcata tgcgcagcga caagggtaga
    14101 caggcgtttc acctgatcct gactcatttc aggcagaatc agcaggccgt ccagccctac
    14161 atggcttgcc ataaaacgaa aagatgcaga tagctggctg tcgcgtacat gctccagtcg
    14221 ttccagacat ggcttaatcg tctcacgtaa atagcgggaa taagcctttg gcctgcccag
    14281 gctgctgaag tattcaatac gttgcatcag cggcttgctg atatgggaag gctgggcgtt
    14341 gacgtccgcc agaatgacca tatccggatt aaaacgctgc tgctcatgcg ccagctttgc
    14401 ccggctaatg agcttatcct gctccatttc gtgctggaca ggatcacgtg attcataaaa
    14461 aaaataacgc tcccagacct gatcactcag tgcctcgcgg cgcaactgtt cctgctcgtt
    14521 atcggcagcg tacagagtga tcaggtttga aagcgcagaa accggcgcaa cttccgccgg
    14581 gtccagataa gggttaatgg cctttttcgg gctgttccat gagaacgctg cggcagcctc
    14641 gttaaagccg cagcagttgt tcatatcggc atggctcatg cacgtactcc gtacacggca
    14701 gaactatcca cgccacgcga ataatcaaat cccacccagc agcgcggccc ggaaacagca
    14761 atgatttctg ttgctgattt accctcgcca gctgccacac cgatgctgcg ttttaccttg
    14821 atatagtggt gagtaaaatt gcgatacagc gaacggatca gggatgtgtc actgttagaa
    14881 acaatgaccg gatgtccttc tgatgaccga tgttcaagaa cggatgctag gtgatactgg
    14941 tcatcttcag tgaaaccatc agtgtgatag ccggaaaacg taccgtcata cggtggatcg
    15001 caatacacca catcccccgc cttcaacatc gccagcgttt catcaaagct ggcgcagata
    15061 aacgttgctc gctgggcttt ttctgcaaat gcgcgaattt ctttttcagg gaaatacgga
    15121 tttttataat tgccgtaggg aatgttgaaa tgcccgctct tgttatagcg acataaacca
    15181 cggtaaccgt gacgattgag atacaggaaa tataccgctt tcatgaaatc agtaatttca
    15241 gttgagtaat taaactcctg ccttatgttg taataagcca cctccctgtt tgcgatctca
    15301 aataaaactc tgg
//
LOCUS       DAJVUJ010000098        14938 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.098, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000098 DAJVUJ010000000
VERSION     DAJVUJ010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14938
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.098"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..452
                     /locus_tag="OQQ48_002719"
     CDS             <1..452
                     /locus_tag="OQQ48_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF945 family protein"
                     /protein_id="HCS1953430.1"
                     /translation="QEVNAALFKEYLPLLQQSEPTIKQPVRWKNALGELNANLDISIA
                     DPAKSSSSTNKDIKSLNFDVKLPLNVVTETAKQLNLSEGMDAEKAQKRADKQISGMMT
                     LGQMFQLITIDNNTASLQLRYTPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(493..1425)
                     /locus_tag="OQQ48_002720"
     CDS             complement(493..1425)
                     /locus_tag="OQQ48_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCS1953431.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQDK"
     gene            1789..4575
                     /gene="polA"
                     /locus_tag="OQQ48_002721"
     CDS             1789..4575
                     /gene="polA"
                     /locus_tag="OQQ48_002721"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCS1953432.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLQQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNPLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRLHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            complement(4957..5589)
                     /locus_tag="OQQ48_002722"
     CDS             complement(4957..5589)
                     /locus_tag="OQQ48_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCS1953433.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRHSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            6171..6680
                     /gene="yihI"
                     /locus_tag="OQQ48_002723"
     CDS             6171..6680
                     /gene="yihI"
                     /locus_tag="OQQ48_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HCS1953434.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            6869..8242
                     /gene="hemN"
                     /locus_tag="OQQ48_002724"
     CDS             6869..8242
                     /gene="hemN"
                     /locus_tag="OQQ48_002724"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCS1953435.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(8432..8542)
                     /locus_tag="OQQ48_002725"
     CDS             complement(8432..8542)
                     /locus_tag="OQQ48_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HCS1953436.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(8653..10062)
                     /gene="glnG"
                     /locus_tag="OQQ48_002726"
     CDS             complement(8653..10062)
                     /gene="glnG"
                     /locus_tag="OQQ48_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HCS1953437.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            complement(10074..11123)
                     /gene="glnL"
                     /locus_tag="OQQ48_002727"
     CDS             complement(10074..11123)
                     /gene="glnL"
                     /locus_tag="OQQ48_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HCS1953438.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLEIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            complement(11297..12706)
                     /gene="glnA"
                     /locus_tag="OQQ48_002728"
     CDS             complement(11297..12706)
                     /gene="glnA"
                     /locus_tag="OQQ48_002728"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HCS1953439.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            13079..14902
                     /gene="typA"
                     /locus_tag="OQQ48_002729"
     CDS             13079..14902
                     /gene="typA"
                     /locus_tag="OQQ48_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000570679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HCS1953440.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDY"
BASE COUNT         3863 a         3746 c         3850 g         3479 t
ORIGIN      
        1 tgcaagaagt gaatgctgca ttgttcaaag agtatttacc gttattacaa caaagtgagc
       61 cgaccattaa acaaccggta agatggaaga acgcactcgg cgaactaaat gccaatctgg
      121 atatcagtat tgccgaccca gccaaatctt catcatccac aaacaaagat atcaaatcgc
      181 tcaattttga tgtgaagtta ccgcttaatg tcgtcacaga aaccgcaaaa cagcttaatt
      241 tatctgaagg aatggatgcg gaaaaagcgc aaaagcgggc tgataaacaa atcagcggga
      301 tgatgacctt aggtcagatg tttcagttaa tcacgattga caacaatacc gcctcgctgc
      361 aactgcgtta tacaccgggt aaagttgttt ttaacggaca ggagatgagc gaagaagaat
      421 ttatgtctcg tgccggacgt tttgttcatt aaaatactca ctcgcctctg ctcattcaga
      481 ggcgagcaaa atttacttgt cctgccgctg tgatgacatc aacgacgtca gaagtcggtc
      541 tttttcctgc cacagtgagt tcagccattg ctgaaaatgg cgcttaaagc ttttgtcatt
      601 gatgtagtcg ccatgtaact catcggcaat aggctgtaaa tccacatgaa cgacaattcg
      661 cgtcagttta ccgcttaaca tatcgaagaa cggctgacgg ttattgtccg gataacacag
      721 cgtaacatta agcagtttat cgaattgttt acccagtaca tttagcgcca tcgcaatgcc
      781 tgccgccttt ggtggcaaca agttttgaaa agtggagtgg gtttgctgat gtttttcttg
      841 cgtgaaacgg gagccttcaa cgaaattgac aatggtggtg ggatgcaggc gaaacttttc
      901 acaagaacgc cgagtggttt caacatcttt accacgtcgc tccggatgac gtaataaata
      961 agcgcgggaa taacgcttca taaacggcat atccagcgcc cagcacgcca ggccaagaaa
     1021 tggcacccag gccagctgtt gcttgaggaa atatttattc atcggaatgt gcttacgaaa
     1081 cagcacgcac agtacgacaa tatctgccca actacggtgg ttacaaataa gcagatacca
     1141 gttcttctta cttaaccctt ccagcccgtg aacttcccat tgcaggtgtg ggttcagatg
     1201 cagtaatacc gccagacctt cacaccagca atacatcata aaatcacaaa aacgcgaaac
     1261 ctttcgccaa atgactggca caggtagcaa tagttttaca atcccggcaa tgatgatcgg
     1321 gacggaacaa aaaatagtga ccaaaatggt caatacgata ctcagcaaaa gggttatcgc
     1381 agcgagtatt ctcgtcataa tgaatttatt caaaaggtta gccatagaca gtgcgctaaa
     1441 gaagcgcaag gcgcagattt tagcagaaaa cgacccaaat aacggatgat ccttaaggag
     1501 aaaaataatt catatctatc cacattagaa aaaatcccat tatctcaatt attagggatg
     1561 gctttatttt taactacatg aaaaacaaag acaaacatca tactgtaaaa agcatgataa
     1621 taaattaaaa gcgatgtaaa taatttatgc acaaagttat ccacatgacg atttgcgagc
     1681 gatccagaag atctacaaaa gattttcacg aaaagcggtg aaaaactcat gtttttatcc
     1741 tgtctgtggc atcctttacc cataatctga taaacaggca cggacattat ggttcagatc
     1801 ccccaaaatc cacttatcct tgtagatggt tcatcttatc tttatcgcgc atatcacgcg
     1861 tttcccccgc tgactaacag cgcaggcgag ccgaccggtg cgatgtatgg tgtcctcaac
     1921 atgctgcgca gtctgatcat gcaatataaa ccgacgcatg cagcggtggt ctttgacgcc
     1981 aagggaaaaa cctttcgtga tgaactgttt gaacattaca aatcacatcg cccgccaatg
     2041 ccggacgatc tgcgagcaca aatcgaacct ctgcacgcga tggttaaagc gatgggactg
     2101 ccgctgctgg cggtttctgg cgtagaagcg gacgacgtta tcggtactct ggcgcgcgaa
     2161 gccgaaaaag ccgggcgtcc ggtgctgatc agcactggcg ataaagatat ggcgcagctg
     2221 gtgactccaa atattacgct tatcaatacc atgacgaata ccatcctcgg accggaagag
     2281 gtggtgaata agtacggcgt gccgccagaa ctgatcatcg atttcctggc gctgatgggt
     2341 gactcctccg ataacatccc tggcgtaccg ggcgtcggtg aaaaaaccgc gcaggcattg
     2401 ctgcaaggtc tgggcgggct ggatacgctg tatgccgagc cagaaaaaat tgctgggttg
     2461 agcttccgtg gcgcgaaaac aatggcagcg aagctccagc aaaacaaaga agttgcttat
     2521 ctctcatacc agctggcgac gattaaaacc gacgttgaac tggagctgac ctgtgaacaa
     2581 ctggaagtgc agcaaccggc agcggaagag ttgttggggc tgttcaaaaa gtatgagttc
     2641 aaacgctgga ctgctgatgt cgaagcgggc aaatggttac aggccaaagg ggcaaaacca
     2701 gccgcgaagc cgcaggaaac cagtgttgca gacgaagcgc cagaagtgac ggcaacggtg
     2761 atttcttatg acaactacgt caccatcctt gatgaagaaa cactgaaagc gtggattgcg
     2821 aagctggaaa aagcgccggt atttgcattt gataccgaaa ccgacagcct tgataacatc
     2881 tctgctaacc tggtcgggct ttcttttgct atcgagccag gcgtagcggc atatattccg
     2941 gttgctcatg attatcttga tgcgcccgat caaatctctc gcgagcgtgc actcgagttg
     3001 ctaaaaccgc tgctggaaga tgaaaaggcg ctgaaggtcg ggcaaaacct gaaatacgat
     3061 cgcggtattc tggcgaatta cggcattgag ctgcgtggga ttgcgtttga taccatgctg
     3121 gagtcctaca ttctcaatag cgttgccggg cgtcacgata tggacagcct cgcggaacgt
     3181 tggttgaagc acaaaaccat cacttttgaa gagattgcgg gtaaaggcaa aaatccactg
     3241 acctttaacc agattgccct cgaagaggcc ggacgttacg ccgccgaaga tgctgatgtc
     3301 accttgcagt tgcatctgaa aatgtggccg gacctgcaaa aacacaaagg gccgttgaac
     3361 gtcttcgaga atatcgaaat gccgctggtg ccggtgcttt cacgcattga acgtaacggt
     3421 gtgaagatcg atccgaaagt gctgcacaat cattctgaag agctcacctt gcgtctggct
     3481 gagctggaaa agaaagcgca tgaaattgca ggtgaagaat ttaacctttc ttccaccaag
     3541 cagttgcaaa ccattctgtt tgaaaaacag ggtattaaac cgctgaagaa aacgccgggt
     3601 ggcgcgccgt caacgtcgga agaggtactg gaagaactgg cgctggacta tccgttgcca
     3661 aaagtgattc tggagtatcg tggtctggcg aagctgaaat cgacctacac cgacaagctg
     3721 ccgctgatga tcaacccgaa aaccgggcgt ttgcatacct cttatcacca ggcagtaact
     3781 gcaacgggac gtttatcgtc aaccgatcct aacctgcaaa acattccggt gcgtaacgaa
     3841 gaaggtcgtc gtatccgcca ggcgtttatt gcgccagagg attatgtgat tgtctcagcg
     3901 gactactcgc agattgaact gcgcattatg gcgcatcttt cgcgtgacaa aggcttgctg
     3961 accgcattcg cggaaggaaa agatatccac cgggcaacgg cggcagaagt gtttggtttg
     4021 ccactggaaa cagtcaccag cgagcaacgc cgtagcgcga aagcgatcaa ctttggtctg
     4081 atttatggca tgagtgcttt cggtctggcg cggcaattga acattccacg taaagaagcg
     4141 cagaagtaca tggaccttta cttcgaacgc taccctggcg tgctggagta tatggaacgc
     4201 acccgtgctc aggcgaaaga gcagggctac gttgaaacgc tggacggacg ccgtctgtat
     4261 ctgccggata tcaaatccag caatggtgct cgtcgtgcag cggctgaacg tgcagccatt
     4321 aacgcgccaa tgcagggaac cgccgccgac attatcaaac gggcgatgat tgccgttgat
     4381 gcgtggttac aggctgagca accgcgtgta cgtatgatca tgcaggtaca cgatgaactg
     4441 gtatttgaag ttcataaaga tgatgttgat gccgtcgcga agcagattca tcaactgatg
     4501 gaaaactgta cccgtctgga tgtgccgttg ctggtggaag tggggagtgg cgaaaactgg
     4561 gatcaggcgc actaagattc gcctgaacat gccctttttt cgtaagtaag caacataagc
     4621 tgtcacgttt tgtgatggct attagaaatt cctatgcaac aactgaaaaa aaattacaaa
     4681 aagtgctttc tgaactgaac aaaaaagagt aaagttagtc gcgtagggta cagaggtaag
     4741 atgttctatc tttcagacct tttacttcac gtaatcggat ttggctgaat attttagccg
     4801 ccccagtcag taatgactgg ggcgtttttt attgggcgaa agaaaagatc cgtaatgcct
     4861 gatgcgctat gtttatcagg ccaacggtag aattgtaatc tattgaattt acgggccgga
     4921 tacgccacat ccggcacaag cattaaggca agaaaattat tcgccgtcct gcgtttcttc
     4981 tacaggctgc atctcgctaa accaggtatc cagtttctgc cgcagcttgt ccacgccttg
     5041 tttcttcaac gaagaaaacg tttcaacctg cacatcaccg ttaaacgcca gtacagcttc
     5101 acgcaccata ttcaattgcg ctttacgtgc gccgcttgcc agtttgtccg ctttggtcag
     5161 cagcaccaga acggcgatat tgctgtctac cgcccactca atcatctgct gatccaaatc
     5221 tttcagcgga tggcgaatat ccattagcac caccagacct tgcaggctat gacgtttttc
     5281 gaggtattcg ccgagcgcac gctgccattt gcgcttcatc tcttccggga cttccgcata
     5341 accgtaccca ggcaagtcaa ccagacgctt gccgtcagcc acttcaaaca ggttgataag
     5401 ctgggtgcgc cctggggttt ttgaggtacg agccaggctt ttctggttag tcagcgtgtt
     5461 cagcgcgctg gatttacctg cgttggaacg gcctgcaaaa gccacttcaa ttccggtatc
     5521 ggaaggtagg tggcgaatat caggcgcact catcacaaaa tgcgtctgtt gataattcaa
     5581 attagtcaaa gcggtcgtct ccgtcagtca aagctttgtg gcgattatac ctgaaccata
     5641 ataaaaggcg gatttttcgg cgtgagcgtt gtaagtaaaa gccatacgct ttgtgagaca
     5701 ttgccgatag tctttatgcg aaatagcaga gaaaattctg cgatgcatgt caaataagct
     5761 atataaatca gtgaattgct tttatataaa actctgaaaa tgtgcaaata ctgatggcgg
     5821 ttgattgttt gtttaaagcg aaggcgtaaa gtagcaccca tagagcgagg acgctaacag
     5881 gaacaatgac tcaggatgag ggtcaggagc gccaggaggc gaagacagag gattgtcagg
     5941 aagacaaacg tccggagacg taattaaacg gaaatggaat caacacggat tgttccggct
     6001 aaaggaaaaa cagggtgtgt tggcggcctg caaggattgt aagacccgtt aagggttatg
     6061 agtcaggaaa aaaggcgaca gagtaatctg tcgccttttt tctttgcttg ctttctgtta
     6121 gattccgccg caaatcgata ctgaataaaa cggctaaaag acgaaccatt atgaaaccat
     6181 catcttcaaa ctcacgcagc aaaggtcatg caaaagcgcg tcgaaaaaca cgcgaggagc
     6241 tggatcagga agctcgtgat cgcaagcgtc agaaaaaacg tcgtggtcat gcgccgggca
     6301 gccgcgcagc gggcggaaac accacgtcag gcagcaaagg ccagaacgca ccaaaagatc
     6361 cacgtattgg cagtaaaacc cctattccat tgggcgtgac tgaaaaagtc accaaacagc
     6421 acaaaccgaa gagtgagaaa cctatgcttt caccgcaggc ggagttggaa ttactggaaa
     6481 cggatgagcg tctggatgcg ctgctggaac gtctggaagc aggcgaaacc ctgagtgccg
     6541 aagagcaatc ctgggtggat gctaaactgg atcgtattga tgagttgatg cagaaactcg
     6601 gcctctctta tgacgatgac gaagaagagg aagaagacga gaagcaagaa gacatgatgc
     6661 gtctgctgcg gggcaactaa cggattgccg ccgtgggcct tcccgttctg ctgataaccc
     6721 ttccggttat atgttatctg gtgtggttat tcgttaaact acagcggttg tcgcggcgac
     6781 aaaagtggct gcgcaaccgg ctgatgaccc gaaacgggca tccgccggta cgccgtagcc
     6841 gccagagacg ccatcggaag gagtgagcat gtctgtacag caaatcgact gggatctggc
     6901 cctgatccag aaatataact attccgggcc acgatacacc tcgtacccga ccgcgctgga
     6961 gttttcagaa gacttcggcg aacaggcgtt tttacaagcc gtggcgcgct atcctgagcg
     7021 tccattatct ctctacgtac atatcccgtt ctgccataag ctttgttact tctgcggttg
     7081 caataagatt gttactcgcc agcagcacaa ggccgatcag tatctggacg cgctggagca
     7141 agaaatcgtc catcgtgcac cgctgttcgc cgggcgtcac gtcagccaat tgcactgggg
     7201 cggcggaacg ccgacgtatc tgaataaagc gcaaatcagc cgcctgatga agctgctgcg
     7261 cgaaaacttc cagttcaatg ccgatgcgga gatttcgatc gaagtcgatc cgcgggaaat
     7321 cgaactggat gtactcgatc atttacgcgc cgaaggcttt aatcgcctga gcatgggcgt
     7381 gcaggacttc aacaaagaag tgcaacgtct ggttaaccgc gagcaggatg aagagttcat
     7441 ctttgcactg cttaaccatg cgcgtgagat tggttttacc tccaccaaca tcgacctgat
     7501 ttacggcctg ccgaaacaga cgccggagag tttcgccttt accctgaaac gtgtggcgga
     7561 gctgaacccc gatcgtctga gtgtctttaa ctacgcgcat ctgccgacca tttttgctgc
     7621 tcagcgcaaa atcaaagatg ctgacctgcc gagtccgcag caaaaactcg atatcctgca
     7681 ggaaaccatc gccttcctga cgcaatcggg ctatcagttt atcggtatgg atcactttgc
     7741 ccgtccggat gacgagctgg cggtggccca gcgtgaaggc gtgctgcatc gtaacttcca
     7801 gggctacacc actcagggcg ataccgatct gctggggatg ggcgtttccg ccatcagcat
     7861 gattggcgac tgctacgcgc agaaccagaa agagttgaag cagtactatc agcaagtgga
     7921 tgaacaaggc aatgcgctgt ggcgtggtat tgcgctaacg cgtgatgact gtattcgccg
     7981 cgatgtgatt aagtcgctca tctgcaactt ccgtctggat tacgccccta ttgagaaaca
     8041 gtgggatttg cacttcgctg attactttgc ggaagatctc aagctgctcg ccccgttagc
     8101 aaaagatggg ctggtggatg tggatgagaa gggaatacag gtgacggcga aaggtcgctt
     8161 gctgatccgc aacatttgca tgtgctttga tacctatctg cgccagaaag cgcggatgca
     8221 gcagttctca cgggtgattt gaatagtgaa tggcgcttcg tttacaaagt agaaaacgaa
     8281 agcgccatca atgtgctgag aacaagattg cctgatgcgc tacgcttatc aggcctacaa
     8341 aacctgttga aaatattgct tttgtaggcc ggataaggcg ttcacgccgc atccggcatg
     8401 aacaaagagc acttcatcag caatctgaaa ctcaactaaa cagcccaatc attgcggcac
     8461 acagcacggc agcaacggca gtttgcggtg tgtggctgtc aactgcgcca ctcgatacca
     8521 gattaattat tgattccagc ataatgactc tccccgtttt ccgggcagat catactgaac
     8581 ttatcggaac agtaaagcgt aaaataccag caatttgcgc tcaataatca atctttacac
     8641 acaagctgtg aatcactcca tccccagctc ttttaactta cgcgtcaggg tgttgcggcc
     8701 ccagccgagt agccgcgccg cttcctgttt atgcccctgc gtatgtcgca acgcggtcgt
     8761 cagtaacgtc cgctccagct ctggctgcgc ttcggaaagc agattttgat gaccggaacg
     8821 cagcgctctg tctgcccact gcgcaagaag cgtcgcccag ctgtccggtt gcatttgcga
     8881 agtactctcc gcaaccgttg attcaaacag ttcgccgggc aaatcctgaa tcaacacttc
     8941 ctgcccggcg gccatcaccg ttagccagcg gcaggtgttt tccagctggc gcacgttgcc
     9001 tggccacgcc agacgcgtca gagcagcttc ggtttccgga tgcagtaact tcgcttctac
     9061 gcccagttcg cgcgcggcaa cctgtaaaaa atggcgcgcc agacggggaa tatcttcccg
     9121 acgttcgcgc agcggcggta gatgaacgcg gataacgttt aggcggtgga acagatcctc
     9181 acggaactta ccttcctgca ctcgctgttc gagattctgg tgagtggcag cgataatccg
     9241 cacatccact ttcaccggcg catagccgcc aacgcggtaa aactgaccgt ctgccagcac
     9301 gcgcagcaaa cgcgtctgca catccagcgg catatcacca atttcgtcga ggaataatgt
     9361 accgccatcg gcctgttcaa aacgcccctg acgaatggta ttcgcgccag taaacgcgcc
     9421 tttctcgtgg ccaaacagtt ctgattcgat caaatctttt gggatagctg ccatattcag
     9481 cgcgataaac ggtgctttgg cgcgcggact gtggcgatgc agggcatgag cgaccagttc
     9541 tttaccggtg ccggattcgc cgttaatcag cacgctaata gaagaacgcg aaagccgacc
     9601 gataatacgg aacacgtcct gcatggctgg cgcttcgccg atgatatcgg tcgttgggcc
     9661 gttaagctga atattacgcg gctgctgctg ttcctggtaa tgactgatag cgcgctcaac
     9721 cagcgccacg gcttcgtcga tatcaaacgg tttgggcaga taatcaaacg ccccttgttg
     9781 ataggcgctg acggcagcat ccagatcgga atgtgcggtc ataatgatga ccggaagcat
     9841 tggatggcgc tgtttaatct gcttgagcag cgccagcccg tccattcccg gcatacggat
     9901 atctgaaagc agcacatccg gcgttttgct cgccagcgcc tccagtactt ccgcgccgtt
     9961 ctcaaatgtc gtacaggtta aacccgctcc agcgagcgca cgttcaagca cccaacggat
    10021 ggaactatcg tcatcgacta cccagactat ccctcgttgc ataaacgtca cctttatttc
    10081 ctgataggca ggtaaaccga gaactcggta tgacctggcc aactggtaaa ttcaattttg
    10141 cctgaatgct gatcaatcaa attacgggcg atggataagc caagcccggt gccaccttcg
    10201 cggccgctga ccatcgggta aaacagcgta tcctgcaaat gaggcggaat gcccggcccg
    10261 ttatcttcca catcaatccg cgccgccagc cggtagcgct cgccgtgtaa ggtcagttga
    10321 aacgcggtgc gggtacgcag aatgatttca ccgccttccg gccccagcgc ctgtagcgca
    10381 ttgcgcacaa tattcagcaa gacctgttca atttgatccg ggtcgtgcgc cagttccggc
    10441 aggctgggat cgtaatcacg aatcaaccgc acgttgtccg gcagttccat cgacaccagc
    10501 gttaccacgc gttcagccac tttgtgaata ctttcggtaa cgcgcgtacc gggcagctgc
    10561 ggccccaaca gacggtcgac cagatttcgc agccggtccg cctgttcgat aatcactttg
    10621 gtatattcga gtagtgatgg gtcaggtaac gctttgctga gcagctgcgc cgcgccacgt
    10681 aaaccgccaa gcggattttt aatctcatgt gccaggccgc gcactaaatc acgggcagca
    10741 acctgctggg cgtgctgtag ctgttcctga cttaagcggc gctggttatc catcggagcc
    10801 atctccagca ggatcatgcc gtccggcata cgctgggccg tcacagaaag gatatgcgag
    10861 cgcccgtcga tgaccagcgt cacttcgtta tcggtaaaac cttgccccgc ctccagactt
    10921 tcttgcatca gctcgatatt taatgagaag tagctcaaca gttccggtaa cggtgtacca
    10981 aacaatttgc gggagctttg ggcgagcagt tgttgcgcgg cagggttggc gtaatggatc
    11041 tccaggttgt catcgattaa caaaatactg ttaatcagcg agttgaggat ctgcccagca
    11101 tcgggctgcg tgcctgttgc cataaagcag tctcctgaac aggttgcacc attttagtgc
    11161 attatagctt tttacggata aaaagcgcga agcatcagag aattgacgga gaaaaaagcc
    11221 catgcagaga tgggctgaaa gtttccacgg caactaaatc ccggcgttgt tgcgccgggt
    11281 agtacaactt caactattag acgctgtagt acagctcaaa ctctaccgga tgcggagtca
    11341 tacgcacgcg gtcatcttct tcgcgacgca gagcgatgta cgcatcaatt gcttcgtcag
    11401 tgaacacgcc accggctttc aggaactcgc ggtccagatc cagttcgttc agtgcttctt
    11461 ccagagagcc tgcaacctgt gggatctctt tcgcttcttc tggcggcagg tcatacaggt
    11521 ttttgtccat ggcttcaccc ggatggatct tgttcttgat accatcaaga ccggccatca
    11581 gcagggcagc aaagcacagg tacgggttag ctgccggatc cgggaaacgt acttcgatac
    11641 gacgtgcttt cggagaagaa accaccggaa tacggataga cgcagaacgg ttacgcgcag
    11701 agtaagccag cattaccggt gcttcatagc ccgggaccag acgcttataa gagttggtgg
    11761 tcgggtttgc cagggcgtta atcgctttag cgtgtttgat tacgccgcca atgtagtaca
    11821 gcgcctgctc agacagacct gcgtatttgt cgcctgcgaa caggttaacg ccgtttttag
    11881 acagagacat gtggcagtgc ataccggagc cgttatcacc gaacatcggt tttggcataa
    11941 aggtcgcggt tttaccgaag cggtgcgcta cgttgtgcac aacatatttg tagatctgaa
    12001 tttcgtcagc ttttttggtc atggtattga agcgggtagc cacttcgttc tgaccagcag
    12061 tcgctacttc gtggtgatgg gcttcaacca ccagccccat ctgttccatc accagacaca
    12121 tttcagaacg aatatcctga gccgagtcta ccggtggaac cgggaagtaa ccgcctttca
    12181 ctgccggacg gtgacctttg ttaccacctt cgtattgggt ggaggagttc catgcgcctt
    12241 cgatatcgtc gatagcaacg tgggaaccgg agatagatga tccgaaacgg atgtcatcga
    12301 acaggaagaa ttcaggttct ggcccgaaca gtactgtgtc ggcaatgcca gtggaacgca
    12361 ggtaatcttc ggcgcgcttc gcaatggagc gcgggtcacg gtcatagcct tgcagggtgc
    12421 caggttcaag gatgtcgcaa cggataatca gggtggagtc ggcgaagaac gggtcaatca
    12481 ctgcggtgga tgcgtctggc atcagcacca tgtcggactc gttaatgcct ttccagccgc
    12541 caatcgagga gccgtcaaac attttgcctt cttcgaagaa ttcagcattc acctgatgag
    12601 cagggatagt gacgtgctgt tctttacctt tagtatcggt gaagcgcaaa tcaacaaact
    12661 tcacttcgtg ctcgttcagc atcgtcagta cgtgttcagc ggacatactt taactctcct
    12721 ggattggtca tggtcgtcgt ggtaacgaaa tctgcaatta ttttggccgt gtcgccgtaa
    12781 aaaagataaa gcgaaatctg tgccaacttt taaattgccc ctaaaaggcg ttatcatgcg
    12841 caccatcgtg caaaagggct gcaccacgat gtgaatgttg caccaatata gtgcttcaat
    12901 ggaaacatta agcaccatgt tggtgcaatg acctttggat aacccttttt atgctccgtg
    12961 aaagcgatca caaagggact ctgcaatact tgtttgcgga ggatgtttgt gatcctgttt
    13021 tgtagtgcga ttaatccgtg tacaataacg cgctatttct aatgcctgag gcaaagttgt
    13081 gatcgaaaaa ttgcgtaata tcgccatcat cgcgcacgta gaccatggta aaaccaccct
    13141 ggtagacaag ctgctccaac aatccggtac gttcgactct cgtgccgaaa cccaagagcg
    13201 cgtgatggac tccaacgatt tggagaaaga gcgtgggatt accatcctcg cgaaaaacac
    13261 cgctatcaaa tggaatgatt accgtatcaa catcgttgat accccggggc acgccgactt
    13321 cggtggtgaa gttgaacgtg taatgtccat ggtagactca gtgctgctgg tggttgacgc
    13381 atttgacggc ccgatgccgc aaacgcgctt cgtaaccaaa aaagcgtttg cttacggcct
    13441 gaagccgatt gttgttatca acaaagttga ccgccctggc gcgcgtcctg attgggttgt
    13501 ggatcaggta ttcgatctgt tcgttaacct cgacgcgacc gacgagcagc tggacttccc
    13561 gatcgtttac gcttctgcgc tgaacggtat cgcgggtctg gaccacgaag atatggcgga
    13621 agacatgacc ccgctgtacc aggcgattgt tgaccacgtt cctgcgccgg acgttgacct
    13681 tgacggtccg ttccagatgc agatttctca gctcgattac aacagctatg ttggcgttat
    13741 cggcattggc cgcatcaagc gcggtaaagt gaagccgaac cagcaggtca ctatcatcga
    13801 tagcgaaggc aaaacccgca acgcgaaagt cggtaaagtg ctgggccacc tcggtctgga
    13861 acgtatcgaa accgacctgg cggaagctgg cgatatcgtt gcgatcacgg gccttggcga
    13921 actgaacatt tctgacaccg tttgcgacac gcaaaacgtt gaagcgctgc cggcactctc
    13981 cgttgatgag ccgaccgttt ctatgttctt ctgcgttaac acctcgccgt tctgcggtaa
    14041 agaaggtaag ttcgtaacgt ctcgtcagat cctggatcgt ctgaacaaag aactggtaca
    14101 caacgttgcg ctgcgcgtag aagaaaccga agacgctgat gcgttccgcg tttctggtcg
    14161 tggcgaacta cacctgtctg ttctgatcga aaacatgcgt cgtgaaggtt tcgaactggc
    14221 ggtatcccgt ccgaaagtta tcttccgtga aatcgacggt cgtaaacaag agccatatga
    14281 aaacgtgact ctggacgttg aagaacagca tcagggttct gtaatgcagg cgctgggcga
    14341 acgtaaaggc gacctgaaaa acatgaatcc agacggtaaa ggccgcgtac gtctcgacta
    14401 cgtgatccca agccgtggtc tgattggctt ccgttctgag ttcatgacca tgacttccgg
    14461 tactggtctg ctgtactcca ccttcagcca ctacgacgac gtacgtccgg gtgaagtggg
    14521 tcagcgtcag aacggcgtac tgatctctaa cggtcagggt aaagcggtcg cgttcgcgct
    14581 gttcggtctg caggatcgcg gtaagctgtt cctcggtcac ggtgcagaag tttacgaagg
    14641 tcagattatc ggtattcata gccgctctaa cgacctgact gtaaactgcc tgaccggtaa
    14701 gaaactgacc aacatgcgtg cttccggtac tgacgaagcc gttgttctgg ttccgcctat
    14761 ccgcatgact ctggaacaag ctctggagtt catcgatgat gacgaactgg tagaagtgac
    14821 tccgacctct atccgtattc gtaaacgtca cctgacggaa aacgatcgtc gtcgcgccaa
    14881 ccgcgcaccg aaagactatt aatttcgttt tttagttata aaaaacctgc catcgctg
//
LOCUS       DAJVUJ010000099        14928 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.099, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000099 DAJVUJ010000000
VERSION     DAJVUJ010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14928
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.099"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..423
                     /locus_tag="OQQ48_002730"
     CDS             <1..423
                     /locus_tag="OQQ48_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCS1953441.1"
                     /translation="TLMNNSDARKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKR
                     LAEFGGLRHKAYRLGGDEFAMVLYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMT
                     LSIGYAMTIEHASAEKLQELADHNMYQAKHQRAEKLVR"
     gene            439..921
                     /locus_tag="OQQ48_002731"
     CDS             439..921
                     /locus_tag="OQQ48_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HCS1953442.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(998..1345)
                     /gene="rplS"
                     /locus_tag="OQQ48_002732"
     CDS             complement(998..1345)
                     /gene="rplS"
                     /locus_tag="OQQ48_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCS1953443.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(1387..2154)
                     /gene="trmD"
                     /locus_tag="OQQ48_002733"
     CDS             complement(1387..2154)
                     /gene="trmD"
                     /locus_tag="OQQ48_002733"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCS1953444.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(2185..2733)
                     /gene="rimM"
                     /locus_tag="OQQ48_002734"
     CDS             complement(2185..2733)
                     /gene="rimM"
                     /locus_tag="OQQ48_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCS1953445.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(2752..3000)
                     /gene="rpsP"
                     /locus_tag="OQQ48_002735"
     CDS             complement(2752..3000)
                     /gene="rpsP"
                     /locus_tag="OQQ48_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCS1953446.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(3137..4498)
                     /gene="ffh"
                     /locus_tag="OQQ48_002736"
     CDS             complement(3137..4498)
                     /gene="ffh"
                     /locus_tag="OQQ48_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCS1953447.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            4665..5456
                     /locus_tag="OQQ48_002737"
     CDS             4665..5456
                     /locus_tag="OQQ48_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HCS1953448.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            5477..6763
                     /locus_tag="OQQ48_002738"
     CDS             5477..6763
                     /locus_tag="OQQ48_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCS1953449.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(6818..7411)
                     /gene="grpE"
                     /locus_tag="OQQ48_002739"
     CDS             complement(6818..7411)
                     /gene="grpE"
                     /locus_tag="OQQ48_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCS1953450.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            7534..8412
                     /gene="nadK"
                     /locus_tag="OQQ48_002740"
     CDS             7534..8412
                     /gene="nadK"
                     /locus_tag="OQQ48_002740"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HCS1953451.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCKKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            8498..10159
                     /gene="recN"
                     /locus_tag="OQQ48_002741"
     CDS             8498..10159
                     /gene="recN"
                     /locus_tag="OQQ48_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCS1953452.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMAYGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALATQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            10308..10649
                     /gene="bamE"
                     /locus_tag="OQQ48_002742"
     CDS             10308..10649
                     /gene="bamE"
                     /locus_tag="OQQ48_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HCS1953453.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(10711..11001)
                     /locus_tag="OQQ48_002743"
     CDS             complement(10711..11001)
                     /locus_tag="OQQ48_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HCS1953454.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(10991..11428)
                     /gene="ratA"
                     /locus_tag="OQQ48_002744"
     CDS             complement(10991..11428)
                     /gene="ratA"
                     /locus_tag="OQQ48_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HCS1953455.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            11599..12081
                     /gene="smpB"
                     /locus_tag="OQQ48_002745"
     CDS             11599..12081
                     /gene="smpB"
                     /locus_tag="OQQ48_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCS1953456.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            12296..12658
                     /gene="ssrA"
                     /locus_tag="OQQ48_002746"
     tmRNA           12296..12658
                     /gene="ssrA"
                     /locus_tag="OQQ48_002746"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(12819..14928)
                     /locus_tag="OQQ48_002747"
                     /pseudo
     CDS             complement(12819..14928)
                     /locus_tag="OQQ48_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
BASE COUNT         3663 a         4017 c         3514 g         3734 t
ORIGIN      
        1 acgttgatga acaattccga tgcccgaaaa acgtcggcgt tactatttct tgatggcgat
       61 aatttcaaat atatcaatga tacctggggt catgcgacgg gcgatagggt cttgattgaa
      121 atcgcaaaac ggttagctga atttggcggg ctgcgacata aagcataccg cctgggcggc
      181 gatgaattcg ctatggtgct ctatgatgta cagtcggaat ctgaagtgca acagatatgc
      241 tcagcactga cacaaatctt taatctcccg tttgatcttc ataatggcca tcagaccacc
      301 atgacattaa gcattggtta cgcgatgacc attgagcacg cctctgcgga aaaattacaa
      361 gagcttgccg atcacaatat gtatcaggcc aaacaccagc gtgccgaaaa gctggtgaga
      421 taacaaggat atatcgatat gataaagcac ctggtagccc ccctgatttt cacctcacta
      481 atactgactg gctgccagtc ccctcaggga aagtttactc ctgagcaagt cgccgctatg
      541 caatcttatg gatttactga atccgccggc gactggtcgc tgggcttatc agatgccatt
      601 ctgttcgcaa aaaatgacta caaattgctc ccggaaagcc agcaacagat ccaaaccatg
      661 gcagccaaat tggcctcgac agggctaaca catgcccgta tggatggaca caccgataac
      721 tatggtgaag acagttacaa cgaaggctta tcattgaaac gggcgaatgt cgtggccgat
      781 gcatgggcta ttggtggaca aattccacgc agcaatctca ccacacaggg tttaggaaaa
      841 aaatatccca tagccagtaa caagaccgcc cagggccgcg ccgagaaccg ccgcgtcgca
      901 gtggtgatta caacccctta aaacacgagc aaacagacaa aaaaagggcc agccaattgg
      961 ccagcccttc ttaacaggat gtcgcttaag cgaaatctta gttaagacgc tctttgatac
     1021 gagcagcctt accagtacgc tcacgcaggt agtacagttt agctttacga acagcaccac
     1081 gacgtttgac agaaatgctg tcaactaccg gagagtgagt ctggaagaca cgctcaacgc
     1141 cttcgccgtt ggaaatttta cgaacagtga atgcagagtg cagaccgcgg ttacgaatag
     1201 cgataaccac gccctcgaat gcctgcagac gttttttgga accttcaaca acccatactt
     1261 tcacttccac ggtatcaccc ggacggaagg aaggtacgtc ctgcttcatc tgctcttgtt
     1321 caagttgctt aataatgttg ctcataattt aatctcttat cctgggtaaa ctgatatctc
     1381 gggggcttac gccatcccat catgtttatg ttgctgttgt gcgtgttccg ttttgaactc
     1441 cgccagcaac cttgcttgct cttcagtcag agccaggttt tccagaagtt caggtcttct
     1501 aagccaggta cggcccagcg actgtttcaa acgccagcga cgtatctcgg catggttgcc
     1561 cgacagtaac actggcggaa cttccatccc ttctaacacc tcaggccgcg tatagtgcgg
     1621 gcaatccagc aatccttcag caaaggaatc ttccgttgcc gaggcttcat gtcccagtac
     1681 ccccggaata aaccgggaaa cggagtcaat cagcgtcatt gctggtaact caccaccact
     1741 gagaacgtaa tcgccgattg accattcttc gtcaatttcg gtttggatca cgcgctcatc
     1801 tataccttcg tagcgaccgc acaccagaat caatttttga ttcgttgcca attcgctgac
     1861 gcccgcttga tcaagcttgc gtccctgtgg tgacagataa atcacctttg cgccttcacc
     1921 cgccgcggct tttgctgcat gaatggcgtc ccgcaagggt tgcaccatca ttaacatccc
     1981 cggtccgccg ccgtaaggac gatcgtccac ggtacggtgc cggtcatgcg tgaagtcgcg
     2041 aggactccag ctctggatgc tcagcaggcc atttttaact gcccggccag ttaccccgta
     2101 atcggtaatt gcgcggaaca tttcaggaaa caggctaatt atgccaatcc acatagcgcc
     2161 gtcttttacc gtttatccgg tggtttaaaa accaggatcc caatctactt cgattgaacg
     2221 agtagtgaga tcgactttct tgataacctg cccatcgagg aacggtacga gacgttcctt
     2281 gataccaaac gcatctttca ggtttgcctt aatgacgaga acgtcattag atccggtttc
     2341 catcatatcg acgactttac cgagatcgta gccttcagtg gttactacct ggcagcccat
     2401 caggtctttc cagtagtagt caccctcttc aagctgaggc aactgcgatg aatccacgac
     2461 aatttcacaa ttcgtcagca ggttcgccgc atcacgatcg tcaacgcctt tcagcttgat
     2521 gatcatgtcc tgattgtggt gcttccagct ttccagctgg acttgctgcc actgacccgc
     2581 cttctggata aaccagggct gatagtcaaa aatgctttcg gcgtcttcgg tggaagaaaa
     2641 cactctgagc cacccacgaa taccgtaaga cgaacccatt tttcccaaaa cgatgggatc
     2701 aacaggtgct tgcgcggtga gttgtttgct catcatgacc accgtgacag attaagctgc
     2761 tttgtttact tctttgatca gcgcagcaac gcgatcagaa atagttgcgc cctggccaac
     2821 ccagtgagcg atgcgatcca gatccaggcg agtgccttct tctttttcgc tagcgattgg
     2881 gttgaagaaa ccaacgcgct cgatgaagcg accgttgcgt gcattacggc tgtcagcgac
     2941 aacaacctgg tagaacggac gctttttagc gccgtgacgt gctaaacgaa tagttaccat
     3001 aacatcctct tgtgtgaata aaacaaccgg accccatcga ggaacggagt ccggtgtcat
     3061 attaaaagcc cgaaaatttt actgatttct ggagaaaatg caatcagcag ttaataactc
     3121 tgtcgcataa ggtcatttag cgaccaggaa atcccggcgg catcatcccc ttcatgcttc
     3181 tcatcatctt cgccattccg cccttcttca ttttcttcat catgcgctgc atgtcgtcga
     3241 actgtttcag aagacggtta acgtcctgca cctgcatacc gcaaccggca gcaatacggc
     3301 gtttacgcga acctttgatg atttctggct tagcgcgctc tttcatcgtc atcgagttga
     3361 tgatggcttc catacgcacc agcactttat cgtccatctg tgacttgacg ttatccggga
     3421 tctgccccat gcccggcagc ttgcccatca ggctggccat gccgcccata tttttcatct
     3481 ggcgcagctg ctcaagaaag tcgttgagat cgaagccgtc accttttttc agcttgctgg
     3541 ctaatttctc tgcctgcgcg cggtcaactt tgctttcgat atcttcgatc agcgacagta
     3601 cgtcgcccat gccgagaata cgcgacgcga tgcggtccgg atggaacggc tccagcgcct
     3661 cagttttctc gccaacaccg aggaacttga tcggtttgcc agtgatgtga cgaatagaga
     3721 gcgccgcacc gccgcgggca tcgccgtcca ctttggtcaa cactacgccg gtaagcggta
     3781 acgcttcatt gaatgctttt gccgtattgg ccgcatcctg accggtcatg gcgtcaacca
     3841 caaacagggt ttcaaccggg ttaatcgacg catggacttg tttgatctcg tccatcatcg
     3901 cttcgtcaac gtgcagacga ccagcggtat ccaccagcag cacgtcgtag aatttcagtt
     3961 tggcttcttt cagcgccgcg ttaacgatat ctaccggctt ctgaccaaca tcagaaggga
     4021 agaaatcaac gcccacctgc tctgccagcg tctcaagctg tttgattgcc gccgggcgat
     4081 aaacgtcggc agaaaccacc agcactttct tcttgtgctt ctcgcgcagg aacttaccga
     4141 gtttaccaac gctggttgtt ttaccggcac cttgcaggcc cgccatcagc acgaccgcag
     4201 gcggttgcgc cgccaggttc agggtctggt tctcttcgcc catcgccgca accagttcgt
     4261 tacggacgat tttgacgaac tcctgccccg gcgtcaggct cttattaact tcatgaccaa
     4321 ccgctttctc ttttacgcga ttgataaact cacgcactac cggcagagcg acatccgcct
     4381 ccagcagcgc catgcgcact tcgcgcagcg tatcttttac gttgtcttca gttaggcgtc
     4441 cacggccact gatattgcgc agcgtgcgcg acaaacgatc ggttaaatta tcaaacattg
     4501 tctctcgcct ggggtggaaa cggttggccg caatcgcgac acatcatcag tattttgccg
     4561 cagtataaca tgaaggcgtc tttgttgtta tgcaacggtt ggagcagcgt tcacctgacg
     4621 ctatactgct tctctttctt attgctcaaa ctgttgacat cactatgccc gtttttgctc
     4681 tgctcgcgct tgtcgcctac tccgtcagtc ttgcgctgat tgttcccggt ctgctgcaaa
     4741 aaaacggcgg ctggcggcgc atggctatta tttctgcggt cattgcgctg gtctgccacg
     4801 caatcgctct ggaagcccgc atcctgcccg acggtgatag cggacaaaac ctcagcctgc
     4861 tgaacgttgg ttcattggtc agtttgatga tctgtacggt aatgaccatt gtggcttctc
     4921 gcaatcgtgg ctggttgctg ctacccattg tctatgcctt tgcgcttatc aacctggcgc
     4981 tggcgacctt catgcccaat gaatacatca cccatctgga agcaacgcct gggatgctgg
     5041 tgcacattgg cttatcgctc ttttcctatg ccacgctaat tatcgccgcc ctgtacgcgc
     5101 tgcaactggc gtggattgat taccaactga agaacaagaa gctggcattt aaccaggaaa
     5161 tgccgccatt gatgagtatc gagcgtaaaa tgttccacat cacgcagatt ggcgtggtgc
     5221 tgctaacgct cacgctttgc actggcctgt tctacatgca caacctattt agcatggaaa
     5281 atatcgacaa ggctgtgctc tctatcgtgg cgtggtttgt ctatattgtg ctgctgtggg
     5341 gacattacca tgaaggatgg cgtggacgcc gcgtcgtctg gtttaacgtt gcgggcgcgg
     5401 taattctgac actggcctac ttcggcagcc gaattgtcca gcagttaatc agctaaaccc
     5461 agaaaaggag tttcccctgg aacacatttc tactactacg ttgatcatta ttctgatcat
     5521 catggtggtc atttcagcct atttttccgg gtccgaaacc ggaatgatga ccctcaaccg
     5581 ctatcgtctg cgacatatgg cgaaacaggg taatcgctcg gccaaacgcg tcgaaaaatt
     5641 gctgcgtaag ccagaccgcc tgataagcct ggtgttaatc ggcaataacc tggtcaatat
     5701 tcttgcctcc gcgctcggca ctattgttgg gatgcgtttg tacggcgatg cgggcgtggc
     5761 aattgcgact ggtgtgctga cttttgtcgt actggtattt gctgaggtat tgccgaaaac
     5821 cattgccgcg ctgtacccgg aaaaagtcgc ttatccgagt agttttctgc tggctccgct
     5881 gcaaattttg atgatgccgc tggtctggtt gctgaatgct atcacccgta tgctgatgcg
     5941 catgatgggt atcaaaaccg atatcgtggt tagcggctct ttgagcaaag aagagttgcg
     6001 cactatcgtg cacgaatcac gctcacaaat ttcccgtcgc aatcaggata tgctgctgtc
     6061 ggtgctcgat ctggaaaaaa tgaccgttga tgacatcatg gtgccgcgca gtgaaattat
     6121 cggtattgat atcaacgatg actggaaatc gattctgcgc caactctccc actcacctca
     6181 cggacgcatc gtgctctacc gtgattcgct ggacgacgcc atcagtatgc tgcgagtacg
     6241 tgaagcctgg cgattgatgt cggagaaaaa agagttcacc aaagaaacca tgctgcgcgc
     6301 cgcggacgag atctattttg tgccggaagg tacgccgctc agcacgcagt tggtaaagtt
     6361 tcagcgcaac aaaaagaaag tcggcctggt cgtcaacgag tatggagaca ttcaggggct
     6421 ggtgacggtt gaagatattc tggaagagat tgtcggcgat ttcactacgt cgatgtcgcc
     6481 aacacttgcc gaagaggtca cgccgcaaaa cgacggttcg gtgattatcg atggcaccgc
     6541 caacgtgcgg gaaatcaaca aagcctttaa ctggcatcta ccggaagatg atgcccgcac
     6601 ggttaatggc gtcattcttg aggcactgga agagatccct gtcgcaggca cccgcgtgcg
     6661 tattggcgag tacgatatag atattctcga cgtacaggac aatatgatta agcaggtaaa
     6721 agtttttcct gtgaaaccgc tgcgcgagag tgtggcggag taacgaaaac ggccctgcat
     6781 tcgcatgcag ggccgtgaat tattacgaaa acagaaatta cgcttttgct ttcgctacag
     6841 ttaccatcgc cgcacgaatc gtacgaccat tcagcgtata acccttctgc ataatgccca
     6901 gtacgttacc tggcgcaacg tcatcagatt ccaccattgc gatagcctga tgcacattcg
     6961 ggtccagcgg cacgttagtt tcggcgatca cttcaacacc aaacttacgc acaacatcca
     7021 gcatcgactt cagcgtcagc tcaatgcctt caaccatcgc agacatatcc gggttagctt
     7081 tatcagccac ttccagcgca cgatccaggc tatcaatcac cggcagcaat tcgttgatga
     7141 atttctccag cgcaaatttg tgggcttttt caatatccag ttcagtacgt cgacgcaggt
     7201 tttccatttc ggcttttaca cgcaaaatgc cgtcacgttc acgggtctgg gcttcagcca
     7261 gctgagcttc gagattcgca attttttcat cgcgcggatc cacctgctca gcagaagctt
     7321 ctggctcaac tgcctcaatc tcttcgtgct gatccatgat aatttcttcc ggggcttgcc
     7381 cctcaggcgt tttctgttct ttactactca tgaatttctc cgcgtttttt tcgcattcat
     7441 ctcgctaact tcgcttatta tggggatcag tttcagggtt tcaagggaag cactcacatt
     7501 gtcatcaatc ttcgcaacaa ggacctcgga aaaatgaata atcatttcaa gtgtattggc
     7561 attgtgggac acccacggca ccccactgca ctgacaacac atgaaatgct ctaccgctgg
     7621 ctgtgcaaaa aaggttacga ggtcatcgtt gagcaacaaa tcgctcacga actgcaactg
     7681 aagaatgtga aaactggcac gctcgcggag attgggcaac tagctgatct cgcggtagtc
     7741 gttggtggcg acggtaatat gctgggcgcg gcacgcacac tcgcccgtta cgatattaaa
     7801 gttattggaa tcaaccgtgg caacctgggt ttcctgactg accttgaccc cgataacgcc
     7861 cagcaacagt tagccgatgt actggaaggc cactacatca gcgagaaacg ttttttgctg
     7921 gaagcgcaag tctgtcagca agattgccag aaacgcatca gcaccgcgat aaatgaagtg
     7981 gtgcttcatc caggcaaagt ggcacatatg attgagttcg aagtgtatat cgacgagatc
     8041 tttgcgtttt ctcagcgatc tgatggacta attatttcga cgccaacagg ctccaccgcc
     8101 tattccctct ctgcaggcgg tcctattctg accccctctc tggatgcgat taccctggtg
     8161 cccatgttcc cgcatacgtt gtcagcacga ccactggtca taaacagcag cagcacgatc
     8221 cgtctgcgtt tttcgcatcg ccgtaacgac ctggaaatca gttgcgacag ccagatagca
     8281 ctgccgattc aggaaggtga agatgtcctg attcgtcgct gtgattacca tctgaatctg
     8341 attcatccga aagattacag ttatttcaac acattaagca ccaagctcgg ctggtcaaaa
     8401 aaattattct aattttacgc cagcctcttt actgtatata aaaccagttt atactgtacg
     8461 caataacagt aatggttttt catacaggaa aacgactatg ttggcacaac tgaccatcag
     8521 caactttgct atcgttcgtg agcttgagat tgattttcat agcggcatga ccgtaataac
     8581 tggcgagacc ggcgcgggta aatctattgc aatagatgcc ctcggtcttt gtctcggtgg
     8641 tcgcgctgaa gccgacatgg tgcgtaccgg cgctgctcgc gctgacctgt gcgcccgttt
     8701 ttctctgaaa gatacgccag cggctctgcg ctggctggaa gaaaatcagc ttgaagacgg
     8761 gcatgaatgt ttgcttcgtc gcgtaatcag cagcgatggt cgctcccgtg gtttcatcaa
     8821 cggtacagct gttcctctgt cacaactgcg cgaactgggt cagttgctga ttcagatcca
     8881 tggtcagcac gctcatcaat tactcaccaa acctgagcac caaaaattcc tgcttgatgg
     8941 ctatgccaat gaaacctctc tactgcagga aatgaccgca cgttatcagt tgtggcatca
     9001 aagctgccgt gacctcgcgc atcatcaaca gttaagtcag gaacgcgccg cccgtgcgga
     9061 actgctgcaa taccaattaa aagaacttaa cgaatttaat ccgcagcccg gagagtttga
     9121 acaaatcgac gaagagtaca aacgtctggc gaacagcggt caattgctga ccaccagcca
     9181 gaatgcattg gcattaatgg cctacggtga agacgcaaac ctgcaaagtc agctttacac
     9241 ggctaaacaa ctggtgagcg aattgattgg catggacagc aaactgtccg gcgtacttga
     9301 tatgctggaa gaagctacca tccagattgc tgaagccagc gatgaactgc gccactactg
     9361 cgatcgtctg gatctcgatc ccaaccgact atttgaactt gaacagcgca tctcaaaaca
     9421 gatttcgctg gcacgtaaac atcacgtcag ccctgaggca ttgccacagt attaccagtc
     9481 gctactggaa gaacagcagc aactggacga tcaggccgac tcacaagaaa cgcttgcgct
     9541 ggcggtaacg aaacatcatc agcaggcact ggaaatcgcg cgcgcattac accaacaacg
     9601 ccagcaatat gcagaagaac ttgcacagct gatcaccgac agtatgcatg cgctctcaat
     9661 gccgcatggg cagtttacga tcgatgttaa atttgacgag catcacctgg gcgctgacgg
     9721 tgccgatcgt attgagtttc gggtaaccac caacccaggt cagccaatgc agcctattgc
     9781 caaagtcgca tccggtggtg aattgtcccg catcgcactg gcaacccagg tcatcacggc
     9841 gcgtaaaatg gaaaccccgg cactgatttt tgatgaagtg gatgtaggga ttagcggtcc
     9901 aacagcggca gttgtcggca aactgctgcg tcaactcggc gaatcaactc aggtgatgtg
     9961 tgttacccac ctgccacaag tcgcgggatg tggtcatcaa cactattttg tcagcaaaga
    10021 aaccgatggt gcgatgacag aaacgcatat gcaatccctg aataaaaaag cgcggttaca
    10081 agagctggcg cgcctgcttg gtggcagtga agtcacacgt aatacactgg cgaatgcgaa
    10141 agaactgctt gcagcgtaaa cttttttcct gcttcacggt cagagtaaac agcaaaacgc
    10201 cgtaagaccg gaaagcaaaa ggttttaaag tgatgaaagg tctattatca tcggcatatt
    10261 acagatgagc cacgtactgc tagggcccga aaaggaatca aatcactatg cgctgtaaaa
    10321 cgctgactgc tgcagcagca gtactattga tgttgaccgc aggctgttcc actctggagc
    10381 gagtggttta ccgtcctgac atcaaccagg ggaactatct gaccgctaac gacgtatcca
    10441 aaatacgtgt tggcatgacg caacaacaag ttgcgtacgc attgggtaca ccgctgatgt
    10501 ccgatccatt tggtacgaat acctggttct atgtcttccg ccagcaacca ggtcatgaag
    10561 gtgtaacgca gcaaacgctg acgctgacct ttaacagtag cggtgtgttg accaatattg
    10621 ataacaaacc tgcgctgagt ggtaactaat aataaagttg ctctcaaaga cgttaaaaaa
    10681 ggtgctcaat gagcaccttt tttctgtctg ttatttattc gctgattttt ctgctcgttg
    10741 cctgcgaagc tctttcggat cggcaatgag agggcgataa atctccaccc gatcgccatc
    10801 atgcacacta tcgcttagtt ttgccggacg gctgtaaatg ccgactttgt ttttagttaa
    10861 atcgatatcg gtacgcaatt ccagcaagcc actggcgcga atagcttctt caaccgtcgc
    10921 gccctcctgc agcgtcacgc gctgcaggta ctgcttctca ggtagcgcat aagccacctc
    10981 aacggcaatt ttacctggca ctgtaaacct ctttcgcacg aaccgtaaaa gcctggacca
    11041 tattagacgc cagctcttta aacacgcgac caaaggcgag ttcaatcaac ttattggtaa
    11101 actcaaagtc gagatgaaac tcgatacgac acgcctcctg gctcagcggc gtaaacttcc
    11161 atccaccaat caatttcttg aacggcccat ccaccagatt cataagaata ctttggttac
    11221 tggtcaactg gttgcgggta gtaaacgttt tgctgatccc agccttagag acatctaccg
    11281 ccgcagtcat ctgcccagga gtggactcca gaatccgact tccggtacaa cccggcaaaa
    11341 actgaggata agactgaacg tcattcacta actgatacat ttgctccgcg ctgtagggta
    11401 ccagtgcggt ccggctaatc tgaggcataa ctatttccat caacaaaaat ccaacaaata
    11461 atatcattta tcctgctgta aaaaaaacgc tatcccggcg ctgggtaaca tcgggttcat
    11521 gctaagatag agccttgtcc cccgcaggat tgatatgggg tgttttcgat ttcagattac
    11581 cgatgattca cgacgcttat gacgaagaaa aaagcacata aacctggttc agcgaccatc
    11641 gcgcttaaca agcgcgcccg tcacgaatac tttatcgaag aagagttcga agcgggactt
    11701 gccctgcaag gctgggaagt taaatccctg cgcgcaggaa aagccaatat cagcgacagc
    11761 tacgtccttc tgcgtgacgg agaggcattt ctgtttggcg ctaacatcac gccaatggcc
    11821 gtggcctcca cgcatgtggt gtgcgatcct acccgtaccc gcaagttact tctcaaccag
    11881 cgcgaactgg actcattgta cggtcgcgtc aatcgagaag gctataccgt agtggcgctc
    11941 tccctgtact ggaaaaatgc ctggtgcaaa gtgaaaatcg gcgtcgccaa aggtaaaaaa
    12001 cagcacgata aacgttcaga tatcaaagaa cgcgaatggc aggtggataa agcacgtatc
    12061 atgaaaaacg cccaccgtta aacctgcact ccaattattg accagttcct caccgcgcct
    12121 ccctctccgg cggcgcgaat gaacatctta ttggctatca catccgacac aaatgttgcc
    12181 atcccattgc ttaatcgaat aaaaatcagg ctacatgggt gctaaatctt taacgataac
    12241 gccattgagg ctggtcatgg cgctcataaa tctggtatac ttgccgttac acattggggc
    12301 tgattctgga ttcgacggga tttgcgaaac ccaaggtgca tgccgagggg cggttggcct
    12361 cgtaaaaagc cgcaaaaaat agtcgcaaac gacgaaaact acgctttagc agcttaataa
    12421 cctgcttaga gccctctctc cctagcctcc gctcttagga cggggatcaa gagaggtcaa
    12481 acccaaaaga gatcgcgtgg aagccctgcc tggggttgaa gcgttaaaac ttaatcaggc
    12541 tagtttgtta gtggcgtgtc cgtccgcagc tggcaagcga atgtaaagac tgactaagca
    12601 tgtagtaccg aggacgtagg aatttcggac gcgggttcaa ctcccgccag ctccaccaat
    12661 catgattgga cggtataagg acaacaccaa taaaaacagg aggttagcag tctcagcagg
    12721 acaccgacca gacggtgaag agacataaaa ggatacgcaa aggagccgcg gctcctggtg
    12781 acatgaaagc ccacagatgt gggctttttc gttgatggtc agaacgacca gttcacacca
    12841 gctaccccgt tccacgggga ttccacaccg gcaccatggc tataccccac cccaagatgc
    12901 ccgcttaacg tactgctgaa tgaggcttta atacctgcct ggtatattcc acgtctgccc
    12961 gacaaatcat tgacgaaatt accgtcacta ttcactttca cccggttatc atcgacaaat
    13021 tctttgcgca cagccgcctt cagccacggc tcaacttcca taccgttccc cagacgcatg
    13081 ttgtaactca gcgttgcacc cagttcacga tataaacctg cgggtatcga ctgatttcga
    13141 ttccatgcca ttggataaat gatattcagg gttatcagcg gtgaaccccc gttaacgatg
    13201 catacggcgt caggttccag ttaccatcgg taaatcgcat cccggtttca atgtgaccgc
    13261 ccagcccgtt gctgcgataa ctgccgttgg tggctccacc gctgccaacc gtggagtcgc
    13321 gagcttcggt acctgctaat accaataaat taccgccatt ttccagcaac atattggtcg
    13381 ctaaattgcc ggaaatggaa aaagtgccgt actggtgagt accgctgatt tcaataccgt
    13441 tagccgtgct cgtctggaga gcggcaccgc tgttctggac gatatctgtc gctttgccat
    13501 tatcgttaac tgtcagcgta ccgccttcat tgatctttgt ttttattgcc tctccgttag
    13561 ctgaaactgt ttgtattccg ccgtcgttaa tcgttgtctc attcgccacc ccctcgacga
    13621 tttgttcgcc gccggtgagc gtcgtgcctg tcgcagtggc ttttgttttg acgatctccc
    13681 gtccgcccgt attgacctgt gttttgtcag aagaggtgtc tgactccacg gttaacacgc
    13741 cgccatttgc cagcaggata ttgttcgccg caccctgctc gatgctgaac gcgacgccat
    13801 ccgcgcgtgt tcctgtgacc cgcgtcgccc tggtggttgc aaccaaagcg ccctggctac
    13861 tctgctgtat ccccgttgcg ctgcctttct cccgcacatc gagtgtgccg ccgtcattaa
    13921 gcaccgagtt ttcagccaga ccgccctcat taactacctg tgaaccccca ttaataatgg
    13981 aaccttccgc tgtcccgttt gccataattt gttgtaggcc agagacgata tcggtattga
    14041 ttgccttacc ataattctga acggtttgcg tgccaccatt gatgtgtgtt ttctctgttg
    14101 acccaccatc aacaatttgt tcaccacttt cgatatttgc ctcagtggct aatccatata
    14161 ccgtttgctt gccacctttg atatttgctt tatcagaagt ggcactggca tatattgttt
    14221 gggtgccagc actattgagt acagtgccaa catcttttcc ataaacatcc attttgccgt
    14281 tggcattaat aatcgtatca actgcccggg aaccagtgac gactgttaat gagccagcgt
    14341 tttccagcac tacattttta gcttctgaat tcctgatgta gaaagcatca ccataactgt
    14401 tggttccttt gataagggtt ccggaagttg tggaagcaat taatgcgccg ccggattgtt
    14461 gctcaacatg cttagcctca ccaccgtcct gaacctccag aacgccacca ttattaagtc
    14521 tggtggtatc tgttttagcc tccttctgga caatcagctt accgccagca tcaacggtag
    14581 tatttttcgc cgaggtttta gccactaccg tcagttcgcc ggtattttcc agcacaacat
    14641 aattagcctc ccctccgggt aatagtgaag tgagagagct tgttgtatcc ttcgatatca
    14701 gtccctgcgc ccgtgttggc aactaaagca ctgcccgtct cctggttaac cccatgtgct
    14761 ataccgccgg tatagacaat cagcgagcct ccggcgctaa tattgctgcc agttgccgta
    14821 ccatctttct caacaacctg ccggctcccg gtggaaatat ttgtcgtgtc agctttcccg
    14881 ccgctcttga tattttgtgt tccgctgttg atattggtac ccgtggca
//
LOCUS       DAJVUJ010000100        14559 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.100, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000100 DAJVUJ010000000
VERSION     DAJVUJ010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14559
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.100"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OQQ48_002748"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OQQ48_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCS1953457.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="OQQ48_002749"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="OQQ48_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCS1953458.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="OQQ48_002750"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="OQQ48_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCS1953459.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="OQQ48_002751"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="OQQ48_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCS1953460.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="OQQ48_002752"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="OQQ48_002752"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HCS1953461.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="OQQ48_002753"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="OQQ48_002753"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HCS1953462.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="OQQ48_002754"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="OQQ48_002754"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HCS1953463.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIVEGHELSSVFFYRGGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4598..5320)
                     /locus_tag="OQQ48_002755"
     CDS             complement(4598..5320)
                     /locus_tag="OQQ48_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCS1953464.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="OQQ48_002756"
     CDS             complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="OQQ48_002756"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCS1953465.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6520..6738
                     /locus_tag="OQQ48_002757"
     CDS             6520..6738
                     /locus_tag="OQQ48_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HCS1953466.1"
                     /translation="MQDLSLEARLAELESRLAFQESTIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="OQQ48_002758"
     CDS             complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="OQQ48_002758"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCS1953467.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7472..7672)
                     /locus_tag="OQQ48_002759"
     CDS             complement(7472..7672)
                     /locus_tag="OQQ48_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HCS1953468.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7682..9493)
                     /gene="kefB"
                     /locus_tag="OQQ48_002760"
     CDS             complement(7682..9493)
                     /gene="kefB"
                     /locus_tag="OQQ48_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HCS1953469.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEE
                     DEKPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYY
                     GDATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHE
                     LLQAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHA
                     DTVQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9493..10044)
                     /gene="kefG"
                     /locus_tag="OQQ48_002761"
     CDS             complement(9493..10044)
                     /gene="kefG"
                     /locus_tag="OQQ48_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HCS1953470.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELANHARAYGDWLANPLSPGGR"
     gene            10175..12088
                     /locus_tag="OQQ48_002762"
     CDS             10175..12088
                     /locus_tag="OQQ48_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCS1953471.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALSQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12088..13110
                     /locus_tag="OQQ48_002763"
     CDS             12088..13110
                     /locus_tag="OQQ48_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCS1953472.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEAPAQARHKPRLVIFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACTYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13104..13322
                     /locus_tag="OQQ48_002764"
     CDS             13104..13322
                     /locus_tag="OQQ48_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HCS1953473.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13376..14245
                     /locus_tag="OQQ48_002765"
     CDS             13376..14245
                     /locus_tag="OQQ48_002765"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HCS1953474.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14300..>14559)
                     /locus_tag="OQQ48_002766"
     CDS             complement(14300..>14559)
                     /locus_tag="OQQ48_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HCS1953475.1"
                     /translation="IDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTG
                     RDLKDAAVARAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
BASE COUNT         3598 a         3970 c         3586 g         3405 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 gatgattgtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt cagaccgata gcagcagcga tgtcgcccgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agagaacggc tcgtcatcac ttgcgtgacg ttcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcgttccagt taatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccgga ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccatgcgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgaga acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt cacctatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatgt tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaagc gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggctggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcgaaattt gaccagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtctccttca
     3781 ctgagcagac gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggtgtc tgcggatcta acccgcgttc
     3961 gcgcagtgaa gccgcacaaa cccagcactg ttcaatgtcg tacagaccca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctgaca gcagctgaaa
     4081 aacgccatca gcaataaaga agacggccag atcgtcagtt aatgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgac cagcagctgt accatgaggt gcagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tcccggctga aggtttgacg aagccagccc cagccttcct
     4321 gcttcagttt catcgacgat accgcggcgt aacgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcacttgcc
     4441 ggtgaggtca attggttagc gttatagacc cctccccgat agaaaaagac gctgcttaac
     4501 tcatggcctt ctactatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctggaaat
     4621 caccgctctt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgca tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaaag tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctggtcgaa aggacgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggtgct ggtttcacat ccagcagctc tacgtcaaac
     5581 accagggtag aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt
     5641 ggaataacca gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc
     5701 gggataacac cgtccagacg gaaagaaagc ggttcaccac gggtgtaaga gttgtcgaac
     5761 tctttaccgt cgatcagcgt acctttgtag ttcactacaa cagtatcgct gtctttcggt
     5821 gcttcgcctt taccggcttc tactacctga taaaccagac cagttgaaga ggttttcaca
     5881 cctttctctt tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg
     5941 tctttttcca tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc
     6001 tgttcgatct cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg
     6061 atcagctgat ctttatccag tttgatgccc agtttttctt gttctttaag agagttttcc
     6121 atgtaacgac ccagcgaagc acccagtgca taagctgatt tctgatcgtc atttttgaac
     6181 gctgctttgc tgtcagcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt
     6241 gcatgcaggg caacggccat tgtggtcgcc agcagcgtta ctttaaacag tgatttcatc
     6301 catatctcca ggatcggggc atctcacccc agggttaact attatcagaa gggtactata
     6361 aagcgttgtc gaacaaatct acatacagac acgccctatt atcatctatt ttcagactct
     6421 ttttgtttaa attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa
     6481 gtagaatccg cggcaaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa
     6541 gcacgcctgg cagagctgga gagccgactg gcttttcagg aaagcaccat tgaagaactg
     6601 aacgtcacgg tgaccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg
     6661 ctgaccgaga agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaagaaacg
     6721 ccaccgccac attattgagg cgtaaaaaaa gcggggattc cccgcttttt tgtcaccttt
     6781 tcggtattag tggcaaccac aaccgccgtt gcctttaccg ccacagcagc cttcgccacc
     6841 gtgttcatga ccgtgatcgt ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg
     6901 gtgatgatcg tgcgcgccgt gaacgtgacc atgagccagt tcttcttcgg tcgcttcacg
     6961 aatcgccaca acctcaacgt tgaatttcag gttctgaccg gccagcatgt ggttaccatc
     7021 aaccactacg tgatcgtctt caaccgcagt gatttcaacc ggtaccggcc cctggtcggt
     7081 ttcagccagg aaacgcatac ctacctgcag ttcatcaacg cccataaata cgtctttagg
     7141 aacacgttgc accaggtttt cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac
     7201 atcaaatttg tcgccaactt catgaccttc cagcgccgtt tccaggccag agatcaggga
     7261 accgtgacca tgcaggtagt ccagcggcgc actcaccgga gactcatcaa ccaacacacc
     7321 gtcttctgta cgtacctgat aggccaggct gaccaccagg tcttttgcta ctttcatgat
     7381 atctcctgag catgggaaga atagtggcgc agattgtagc ggaattctgc agccgtgtac
     7441 tcactagctt aaaaaaactc ggcgcatatc gctagtccgg atgaaaaatc ccgatcactt
     7501 gctcatcttt gcgaacgtga tcgcgggctt ctttgtctgc ttctcgcatc tgatgtccgc
     7561 acttaacgca ttcaacaata tcaatattat tttcgcgcca catcgccatt gaatcctgtg
     7621 cctgacaggc cgggcatttt gcgcccgcaa taaaacgttt tcggattgcc atctttaccc
     7681 tctactcaaa ttcatcccag ccgtccagct ggcgtcgttc ttgttgcatt tcacgctgga
     7741 aaatctcttc cagttcgcgt cgggcttccc tggcgcgaga aatttgtaca gtatcggcat
     7801 gcattgggat gagctctcgc agcatccgca tatccaggcg gcgaaaatgc agttgcgccc
     7861 gctgcgcctg atgcggatgc atcccaagcg tgaccaaagt cttgcgcccc agctctaacg
     7921 cactggagaa tgtttcacgg gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt
     7981 ccacacgtcc gcgcgctcgc gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt
     8041 ccaccagctt catggtgtct tccggctcgt tacaggtaat gacgatagac tcagcggcct
     8101 ctgcacccgc agaacgtaat agatcgacct gcgtggcgtc gccgtaataa actttgtagc
     8161 catatttacg catcaggtta acggcgctga tatcccgctc cagcacggta atgcgcattt
     8221 tatttgccat cagcaaacga ccaatcacct gaccaaaacg cccgaagccc acgacaatga
     8281 cctggggttt atcatcgttg acccacggtt tttcgtcttc ttcttccggt ccgttaaact
     8341 ggcgggataa ccatttatcc accagcttca tcagcaacgg cgtggtcatc atggaaagcg
     8401 tcaccgtcac cgtcaccagc aacagcgcca tctggtcgcc ctggaataaa cgttgtgaag
     8461 aagcggtaga aaagaggaca aaggcaaact caccgccctg actcaacacg ccagcaaact
     8521 gcatccgctc tgagctacgc acgccataca atcgcgccag cagatacagc acgagaattt
     8581 tcaccgccac cagcacaacc acgcttatca ctacccacaa cagatgggta taaagcaccc
     8641 cgaggttgag cgacatgccg acagagataa agaacaaacc gagcagcaag cctttgaagg
     8701 gatcgatagc cgtttccagt tcatggcgat attcactttc cgccagcagc acgcccgcaa
     8761 taaatgtacc gagcgccatc gacagcccca gcgcatccat aaacaatgcg gaacccaaca
     8821 ccagcagcag cgtcgcggcg gtgaacactt cccgcacgcc agaagctgca ataaagcgga
     8881 ataccggacg cagtaaatag cgcccaccaa tcagcatgcc gacaaacgcc agcaccttca
     8941 tgccgatctt catccagtcg aaatgttcgt ctgccgaccc cgccaacaac ggcactaacg
     9001 ccagtgctgg gatcactgcc agatcctgaa acagcagaac cgaaaacccg agctggccgg
     9061 attcgctgcg attcatccct ttctcacgca tcaattgcaa cgccattgca gttgaagaca
     9121 tcgcaaggcc aatgccaccg accaccgccg cctgccaggc gaaatccgtc agcatcaata
     9181 atcccgccag caacgccgcg cttaacagca cctgtgccgc gcctacgcca aaaatcgaac
     9241 gccgcagttg ccaaagtttg gagggattca actcaaggcc gatgataaac atcaggaata
     9301 ccacgccgag ttccgaaaag tggaggatct catcgacgtc gctaataaac cccagccccc
     9361 acgggccaat tgcaatccct gccagcaaat atcccaacac agcgccaatc cccagccgcg
     9421 atgccaacgg caccgcagcc accgccgcga agagaaacag cactcctgcg agtaaaaaat
     9481 cggaaccttc catcagcggc ctcctggaga cagcggattt gccagccagt caccgtaggc
     9541 tctggcgtgg ttcgccagct cctttgcgct ttgccgtctc gcccagtaaa taatgatggg
     9601 acttaaccaa tgcatccggc acatgcccgc cgccagttca aaggggcgca gcacatcgct
     9661 catcgggtag cgattcagcg cgtcataacg gtaagcactt tccggctcgc cggtggtaat
     9721 cacgttacgc cagtactttc ccgccagttg gtttcctccc ggcccgctgg caaaaccacg
     9781 acttaatacc cggtccagcc actctttcag tagcgccggg cagctatagg tataaagagg
     9841 atgctgaaag acaatcacct cgtgctcgcg cagtaatgcc tgctcacggg ggatatcaat
     9901 aaaaaaatcg ggatagtgcg cgtaaaggtc gtgcacggta acattgctga gctgcgtggc
     9961 cggtttaagc agtacccggt ttgccaccga gtcctgagat tccggatggg catacagcag
    10021 caaaactttc gctggctgag acatcatccc cctcccggta tggtttttgt gtatagtcgc
    10081 tgttttgggc taccattgcg cccggtgcgg cagctcgccc atacattaca ttatcataat
    10141 gataagttaa catagtctga acatacggcg ccttatgatt gttttctcct cgttacaaat
    10201 tcgtcgcggc gtgcgcgtcc tgctggataa tgccaccgcc accatcaacc ctgggcagaa
    10261 agtcggcctg gtgggtaaaa acggctgtgg taaatctacc ctgctggcat tgctgaaaaa
    10321 tgaaatcagc gccgacggcg gcagctacac ctttccggga agctggcaac tggcgtgggt
    10381 gaatcaggaa acgccggcgt tatcgcaagc ggcgctggaa tatgtcattg acggcgaccg
    10441 tgaatatcgt caactggaag cgcagctgca cgacgccaac gaacgtaacg acgggcacgc
    10501 cattgcgacc attcatggca agctggatgc tattgacgca tggagtattc gctcccgtgc
    10561 cgccagcctg ctgcacggcc tcggtttcag caatgaacaa ctggagcgcc cggtaagtga
    10621 tttctccggt ggctggcgta tgcgtcttaa ccttgcccag gcgctgattt gccgttcaga
    10681 cttgctgctg ctcgacgaac cgactaacca cctcgatctc gatgccgtta tctggctgga
    10741 aaaatggttg aagagctatc aaggcacgct gatcctgatc tctcacgacc gcgacttcct
    10801 cgatccgatc gttgataaaa ttattcatat cgaacaacaa agcatgttcg agtacaccgg
    10861 caactacagt tcgtttgaag tacagcgcgc cacccgtctg gcgcaacaac aagcgatgta
    10921 tgaaagccag caggaacgcg tggcacatct gcaaagttat atcgaccgtt tccgtgccaa
    10981 agccaccaaa gcgaagcaag cccagagccg cattaagatg cttgagcgta tggagctgat
    11041 tgcccccgcg cacgtcgaca acccgttccg ctttagcttc cgcgcgccgg aaagcctgcc
    11101 aaatccgtta ctgaagatgg aaaaagtcag cgcgggctat ggcgatcgca ttattctcga
    11161 ctcgattaaa ctgaacctgg tgcccggctc gcgcattggt ctgttaggcc gcaacggcgc
    11221 gggtaaatcg acattaatca aactgttagc cggtgaactt gcgccagtca gcggtgaaat
    11281 tggtctggcg aaagggatca agctcggcta cttcgcccag catcaacttg aatacctgcg
    11341 cgccgacgaa tcacctattc aacatctggc acgtttagcg ccgcaggagc tggaacaaaa
    11401 actgcgtgac tacctcggcg gctttggttt ccagggcgat aaagtaaccg aagaaacgcg
    11461 ccgcttctcc ggtggggaaa aagcccgcct ggtgctggca ttaattgtct ggcagcgtcc
    11521 gaatctgctg ctgctcgacg aaccgactaa ccaccttgac ctcgacatgc gtcaggcact
    11581 caccgaagca ttaatcgagt ttgaaggcgc gctggttgtc gtttcgcacg accgtcattt
    11641 gctgcgttcc accactgacg atctctacct ggttcacgat cgtaaagtcg aaccgttcga
    11701 cggcgatctg gaagattatc aacagtggtt gagcgacgta caaaagcagg aaaaccagac
    11761 cgacgaagcg ccaaaagaga acgcgaacag cgcccaggca cgtaaagatc agaagcgtcg
    11821 ggaagcggag ctgcgtgcgc aaacccagcc gctgcgtaaa gagattgccc gtctggaaaa
    11881 agagatggag aagctgaacg cgcaactggc gcaggcggaa gagaaactcg gcgacagcga
    11941 actgtatgac cagagccgta aagcggagtt gaccgcctgc ctgcaacagc aagccagcgc
    12001 caaatccggc ctggaagagt gcgaaatggc gtggctggaa gcccaggagc agcttgagca
    12061 gatgctgctg gaaggccaaa gcaactgatg gcgcagataa cgacgaccga tgccaatgaa
    12121 ttcagcagca gtgctgaatt cacccctatg cgcggcttta gcaattgtca tctacaaact
    12181 atgctgccgc gtttgtttcg tcgtcaggtg aaattcaccc cgtactggca gcggctggag
    12241 ttgcccgacg gcgattttgt cgatctcgcg tggagtgaag cccctgcaca ggctcggcat
    12301 aaaccgcgtt tagtgatatt tcacgggctg gaaggcagtc tcaatagccc ttacgcccac
    12361 ggtctggttg aggcggcgca aaagcgcggc tggctgggcg tggtgatgca ttttcgcgga
    12421 tgcagcggtg aaccaaaccg tatgcaccgc atttaccatt cgggcgaaac cgaagacgcc
    12481 agttggtttt tacgctggct gcaacgcgaa tttggtcatg cgccaacggc tgccgtcggc
    12541 tattcgctcg gcggtaatat gctggcctgt ttgctggcaa aggaaggcaa tgatctcccg
    12601 gttgatgcgg cggtgattgt ctctgcgccg tttatgctgg aagcctgtac ctatcatatg
    12661 gaaaagggct tttcccgcgt ttatcagcgt tacttgctga acctgttaaa agccaatgcc
    12721 gcgcgcaagc tggcagccta ccccggaacg ctgccgatta atctcgcgca gttaaaatcg
    12781 gtacgtcgca tccgtgaatt tgacgatcta atcaccgcca gaattcacgg ctacgccgac
    12841 gctatcgact attatcgtca gtgtagcgcc atgccgatgc tgaaccggat cgccaaaccg
    12901 acgctgatta ttcacgccaa agacgatccg tttatggatc atcaggtgat cccgaaaccg
    12961 gaaagtctcc cctcgcaggt ggagtatcaa ctgactgaac atggcggtca tgttggcttt
    13021 attggcggta cattacttca tccgcaaatg tggctggagt cacgcattcc tgactggtta
    13081 acaacgtatc tggaggcgaa atcatgttga ttccgtggca agacctctcc cccgaaacgc
    13141 tggaaaattt aattgaaagc tttgtgttac gtgaaggcac cgattatggt gaacatgagc
    13201 gtacacttga acagaaagtc gccgacgtca aacgccagct acagtgcgga gaagcggtgc
    13261 tggtatggtc ggagctgcac gaaacggtca atattatgcc gcgcagtcag tttcgcgaat
    13321 aacctgcaac cgtcggcgga cgagataata atgatagcta agtcatggag ttactatgtc
    13381 tgccaaacat ccggtcattg cggtaacagg atccagcggc gcggggacca ccaccaccag
    13441 cctcgcgttt cgtaaaatat tcgcgcagtt aaatctgcat gcagctgagg tggaaggcga
    13501 cagttttcac cgttacaccc gcccggaaat ggacatggcg atccgcaaag cgcgcgacgc
    13561 cgggcggcat atcagctact tcggccccga agcgaacgac ttcggcctgc tggaacaaac
    13621 cttcattgaa tacggtcaga gcggcaaagg gaaatctcgc aaatatctgc atacctacga
    13681 cgaagccgta ccgtggaatc aggtaccggg aacattcacc ccctggcaac ctttaccaga
    13741 acccactgat gtactgtttt atgaaggttt acacggcggc gtagtcacgc cacagcataa
    13801 cgttgcgcag catgtggact tactggtcgg cgtagtgcct atcgttaacc ttgagtggat
    13861 tcaaaaactg atccgcgaca ccagcgagcg cgggcactca cgagaagcag tgatggactc
    13921 agtagtgcgt tcaatggaag actatatcaa ctacatcaca ccgcagtttt cccgcaccca
    13981 tcttaacttc cagcgcgttc ccaccgtcga cacttcaaac ccgttcgcgg caaaaggtat
    14041 cccgtcgctg gatgaaagct ttgtggtgat ccattttcgt aatctggaag ggatcgattt
    14101 cccctggctg ctggcgatgt tgcaaggctc attcatttcc cacatcaata cgttagtggt
    14161 accgggcggc aaaatgggtc tggcaatgga attaattatg ctgccgctgg tgcaacgatt
    14221 gatggaagga aagaaaatcg agtaactctg ctattacgcc ggataaaata ctatccggct
    14281 tcacaacggg atagttaaat cacgcggcaa ccacttcata cgagtgagta atattcaccg
    14341 ctttttccag catcaacgcc actgaacaat atttctcggc agagagatca accgcacgcg
    14401 caaccgctgc gtctttcagg tcgcgaccgg tgacgataaa atgcagatta atgtgcgtaa
    14461 acaggcgtgg tgcctcttcg cggcgttcag aggtcaattt tacttcacaa tcgaccacat
    14521 cctgacgccc tttttgcagg atcgaaacca catcgatgg
//
LOCUS       DAJVUJ010000101        14502 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.101, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000101 DAJVUJ010000000
VERSION     DAJVUJ010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14502
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.101"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(<1..787)
                     /gene="fimH"
                     /locus_tag="OQQ48_002767"
     CDS             complement(<1..787)
                     /gene="fimH"
                     /locus_tag="OQQ48_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HCS1953476.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLG"
     gene            complement(807..1310)
                     /gene="fimG"
                     /locus_tag="OQQ48_002768"
     CDS             complement(807..1310)
                     /gene="fimG"
                     /locus_tag="OQQ48_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HCS1953477.1"
                     /translation="MKWRKRGYLLAAILALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGETKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(1323..1853)
                     /gene="fimF"
                     /locus_tag="OQQ48_002769"
     CDS             complement(1323..1853)
                     /gene="fimF"
                     /locus_tag="OQQ48_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HCS1953478.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAASGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(1863..4499)
                     /gene="fimD"
                     /locus_tag="OQQ48_002770"
                     /pseudo
     CDS             complement(1863..4499)
                     /gene="fimD"
                     /locus_tag="OQQ48_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
     gene            complement(4566..5291)
                     /gene="fimC"
                     /locus_tag="OQQ48_002771"
     CDS             complement(4566..5291)
                     /gene="fimC"
                     /locus_tag="OQQ48_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HCS1953479.1"
                     /translation="MSNKNANVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWMENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQFAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(5328..5867)
                     /locus_tag="OQQ48_002772"
     CDS             complement(5328..5867)
                     /locus_tag="OQQ48_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCS1953480.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAVGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPVNWKRLYSGSTSLHFIAKYRATG
                     RWVTGGIANAQAWFSLTYQ"
     gene            complement(5932..6480)
                     /gene="fimA"
                     /locus_tag="OQQ48_002773"
     CDS             complement(5932..6480)
                     /gene="fimA"
                     /locus_tag="OQQ48_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCS1953481.1"
                     /translation="MKIKTLAIVVLSALSLSSTAALAAATTVNGGTVHFKGEVVNAAC
                     AVDAGSVDQIVQLGQVRTASLAQEGATSSAVGFNIQLNDCDTNVASKAAVAFLGTAID
                     AGHTNVLALQSSAAGSATNVGVQILDRTGAALTLDGATFSEQTTLNNGTNTIPFQARY
                     YATGVATPGAANADATFKVQYQ"
     gene            complement(6957..7553)
                     /gene="fimE"
                     /locus_tag="OQQ48_002774"
     CDS             complement(6957..7553)
                     /gene="fimE"
                     /locus_tag="OQQ48_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313299.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HCS1953482.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTLERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
     gene            complement(8031..8633)
                     /gene="fimB"
                     /locus_tag="OQQ48_002775"
     CDS             complement(8031..8633)
                     /gene="fimB"
                     /locus_tag="OQQ48_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HCS1953483.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTPPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
     gene            10089..10805
                     /gene="nanC"
                     /locus_tag="OQQ48_002776"
     CDS             10089..10805
                     /gene="nanC"
                     /locus_tag="OQQ48_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HCS1953484.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSSDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            10825..11931
                     /gene="nanM"
                     /locus_tag="OQQ48_002777"
     CDS             10825..11931
                     /gene="nanM"
                     /locus_tag="OQQ48_002777"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HCS1953485.1"
                     /translation="MNKTITALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNREGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAVDKINAYYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLAPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQHGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            11996..12976
                     /locus_tag="OQQ48_002778"
     CDS             11996..12976
                     /locus_tag="OQQ48_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HCS1953486.1"
                     /translation="MDAIISPDYYYVLTIAGQSNAMAYGEGLPLPAREDAPHPRIKQL
                     ARFAHTHPGGPSCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLSFIPDNAGVLIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHAYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLIMGYYGSAYRSPENWTTALRSSHFSAAARRGIISDRFVEAILQFWRGK"
     gene            complement(12984..13106)
                     /locus_tag="OQQ48_002779"
     CDS             complement(12984..13106)
                     /locus_tag="OQQ48_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012139841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCS1953487.1"
                     /translation="MISFVFLLLVCPNCHAMLHRRKPPFTPEELKALMDANKSN"
     gene            13188..14060
                     /locus_tag="OQQ48_002780"
     CDS             13188..14060
                     /locus_tag="OQQ48_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004186812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCS1953488.1"
                     /translation="MASSKSLQQAIANIKIWHKGEQRAPHKPLLLLYVLAGYLNGHPR
                     LFDYGSEIYEPLHSLLERFGPQRSQYRPDMPFWRLQGDGFWQLHNAELCSTAGSSRQP
                     PVKELNEYHVAGGFDEQHYALVTGNKKLINTLAQQILEAHFTESIQEEIADELGFDLQ
                     QIRKQRDPLFRKNVLRAYNYQCAICGFNMRHDDTTVALEAAHIKWKQHGGPCEIPNGL
                     ALCAIHHKAFDKGSIGLDENMRVLVSDAVNGGGSVERLFWDFDGKIIALPQVRKNYPY
                     EGFVEWHRNEVFRG"
     gene            14105..14284
                     /locus_tag="OQQ48_002781"
     CDS             14105..14284
                     /locus_tag="OQQ48_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1953489.1"
                     /translation="MQDLAASLQLAFDSGAKRVLLPISWAMDIPTVPAELFTKFQVSF
                     YSEPVDAVYKALDVN"
BASE COUNT         4061 a         3346 c         3166 g         3929 t
ORIGIN      
        1 ctcctaacga taccgtgtta ttcgctggaa taatcgtacc gttgcgcgtc aactgtacgc
       61 cgacgccctg cgctggtgaa aacgacgcgg tattggtgaa aatcgagttg cccgcatctg
      121 cggttgtgcc ggagaggtaa taccccaggt tttggctttt cgcacaataa acggtaagag
      181 gaattggcac tgaaccaggg tagtccggca gagtaacggt gacatcacga gcagaaacat
      241 cgcagccgcc agtaggcacc accacatcat tattggcgta aatattccac acaaactgga
      301 aatcatcgct gttatagttg ttggtctgtc gcaaaataag cacggcaatt aatgagccag
      361 ctttaatcgc caccccgccc gcactgctca caggcgtcaa ataaagcgcc accggccacg
      421 gcttatccgt tctcgaatta taaacaaccc gcggcgtttc gctggtggtc ggaaatggat
      481 agctactgcc actatatttt acggtcccgg aaaaattaga taacacgccg ccataagccg
      541 agcctcgttg cagtgtgaca tagtctgtaa tggtttccgg ataatcgtta tggcaaaaga
      601 tttgcgtcga aagatctacg accaggtttt gccccacatt cacgacaggc gcaaggttta
      661 cataaacatt agcgctgcca ccgccaatag ggatagcagt accattggcg gttttacagg
      721 cgaatgacca ggcatttacc gaccagccca tcagcagtac agcaaacagg gtaataactc
      781 gtttcattac aattatctct tcgggttcag ctgtaggtat aggtgatgct aatcactgcc
      841 tgaatggttc cctgagtggc tccgccattt accgtcaatg ctctgacctg taacgggaag
      901 tgcgctgatt gtgaggaatc atccacctga actgttttgg tttcgccagt attcaatgtg
      961 ttgccactgt catcctgtag ctctaactgg atgttttgcg cggtcccctg gtttttataa
     1021 tatccggtac tgtcggctgc cccgctgaag ctggcagtga ccctcgacgt tcccaccgga
     1081 caattagtca actcaagcgc aacatcatgc caggccgatg ccgccccggc agacatcaga
     1141 ctgaaagaat aaagatcgcc gagatcaacc gtggcattgg tggtggaaac tgtgcacggt
     1201 ttggcgacga ccttaccgtt caccgtgatg gtgacatcgg ctgcctgtat tgtcgcactt
     1261 gcgagcgcca atattgccgc caataaatac ccacgtttgc gccatttcat gagcatctcc
     1321 agttactgat attcaagagt gaaggtagcc gtggcattga tatgccccgc agtgacaggc
     1381 acctgtgtcg ccattagccg ggcgtaaaaa ttcaacgtat ttggtttacc cggcgtcagg
     1441 gtcgtccacg aaatcgcgga cgatggagca ttaaggggta tctgattttg ctgctcattc
     1501 agaagctgta ttcccagtcc cgaagccgct gacaccgtat tttcaagtgc aagcaggttg
     1561 gcattgtggc tatctgcaac gccggtaaac ccaaccttta cggcagaaac ggcgttacca
     1621 cagggtgaca gcaaaatacg aaatggaacg acaggagtcg ttgcgccaat gttgttaaat
     1681 tgcttcgccg cgttttccat cagatcaaca gtaaaattgg ttgattcagc ggccacacta
     1741 cagccgttat ctctgacata gccgcggata gtaatcgtgc tatccgcagc caaaacgcga
     1801 cttaccgcca gccacaaaaa agcgcacaga agataaaaag gtttgtttct catcacgccc
     1861 ccttaacgac attcagctga tagctgggtt aataactgct gctgactctc tggtggcagt
     1921 tgataattgg cgatacagtg agcattttcc tcttctcccc atttcacctg aacttttccc
     1981 gctaaaggca ttccgctgag gtaaacctga ccattatccg caacaatgcc gctactctgg
     2041 ctactctctg atgtcaccat cgccccaaac ggcagcggct tattattgtg ggttagcgtc
     2101 atgagcagtt ttatcccaac gcgcgcttta aactctgctc gcacgatcgc cccacgagtg
     2161 ggaacaacgt tagcgaccgc gttatctaaa tcgacgttat cagccagggt attggtatcc
     2221 agcgccactc tgttttcccg atattcggtg gcataaggca gcacggcata accgcgccag
     2281 tcggtacgca cccccgtctg gttttcgact tttgcatctt ttgcgccagg cgctttaaca
     2341 agtaccaccg tatcgtttaa cggctgcccc agcgttacgc cattggcatg agccagtacc
     2401 ccaccgctga ctccgtaata gagctgctta atatcatcgc tatggctgta accgatattg
     2461 gcattgccgt aaccaccgcg ataattcagc gtggcgtagc ctgtacttcc gctattacca
     2521 tcgcctcccc cggcatagcc ggtttgcacg ctatagctga ggttgttgtc ttccagcaac
     2581 gtaccgtata caccagccag attggtcatc cgaccgttga gatcgtgtga cacgctgtag
     2641 ctggcactgg catgtcgcca ctgagattta ctgtcagaac gcagccagtg gctgaaagga
     2701 atattgacgt taagcgctaa catctgatcc cgcccttttt gccaggcgtt tttcgtcagg
     2761 ctatagctga gcgtccagtt gatatcttcg aacgcagtat ttaatccagc ctagaattgc
     2821 tcatcgacat tactcgttcc ccaataagtt tgatggctac cactcaaata cagtgttgat
     2881 gtgcgcccga gttgctgagt aacggtgagc tgtaatttcc cgcgtttgtt ataagcgagg
     2941 ttgtaatagt cggtgaattt cggcttaacc tgaataactc catcctgtgt ttcgatgttg
     3001 tagccattca ttcgactgta tgttgtatca gcgaaattaa aatatccgct ggtcgaataa
     3061 cggtaaccca ctaactgaat attcgtgcct gactcattga gcgatttgtt atagagaaaa
     3121 cgcaccgatt gtccgtcatg ctgactgtca tcgggaagtg tggaattagc ctgcgtcata
     3181 tccacagaca gagcgcccag tgcccccatg tttttcccga taccgaaatt aaaagcacga
     3241 taacgatccg ccagttgcgt tccaccatat attgtccagc cagccggaag gccgtggagt
     3301 aatgtacttt ggaaaaagcg gggtttttcc tgctgcgtat ttccactacg gtattctcct
     3361 gccgtaatgg aataacgagt atgcccttca cgttgcaaaa gcgggactga cgaatagggt
     3421 acggtaaaaa tctgcgtgct gccgtcagcc tctttgatcg ttacctgcaa gtcaccacta
     3481 ttacctgcgg catagatatc gttgatggta aaaggccccg gtggcaccgt actattataa
     3541 atgtcatacc cattttgttt aatagtgacc tgtgcagtac cacgagcaat accgtggatc
     3601 accggggcaa atcctctttg gctatcgggt aacatattgt catctgaggc caattgtgcg
     3661 ccgcgaaagt taataccatc gaaaatatcg ccctgggtat aaccatcacc cagcgtcagc
     3721 cgggaacgta acggtattat gtctcgctca agccaggtat tgatatgctg ccatttattt
     3781 ttgctacctg atgatctgtc gctactgtta taactccagc tggtattgtc gcgtaaacac
     3841 cacgcaccaa tatttaaccc actctgtagg tttaaatatg cataatggct gttaccccca
     3901 atctgattct gtacactatt tccgctgaaa ttataattga gcaatccggc attaataccg
     3961 ggatcccata actcaggagg aatataacca cgcgcgcgat tactcataaa tgcctgaggg
     4021 atcgtcaggt tcagtcgctg ctgaccaaca tccagatgcg cagtagcgtc atggatcatt
     4081 gaggttaatg gcacgcaggc atcatctgcc agcagattca taccggagac agaagccgca
     4141 ttcagcccca tactggcaag ttgcgcgcgt gtcaggcagg gaacaatccc ttgttcactg
     4201 tcgcccgtat taaatgtgac atcacgcgtt gccatataac cattattcaa atagatatcg
     4261 acgcgatacg tccctggcgg taattcttgc ccattttcaa aacgcgataa atcggccaca
     4321 gcctggggat catccgctaa aaagcgcgga ttaaaataga gttcggcaga tgacaaaggt
     4381 gcctgtgcgg caaaagcaca ggcgacaacg agccggacaa aaaaaccagc caaacgatgc
     4441 ttacgaatat gcaagcattg tgtgtttcgc tggtaaagtc ttaaattcag atatgacatt
     4501 attcctccgg tttaaaatta cccggcagtc aattcttttt attcaggcga gaaattcccc
     4561 ctgcgttatt ccattacgcc cgtcattttg ggggtaagtg cgccataatc atttattgtt
     4621 cggtaagtaa tattgcttcc tgcatcagaa ggcaatttaa ccgtgctttc gcccattgga
     4681 ggcaccaatg cattttcaag aacccgggtt ccggcattca actctgttac cgtcagataa
     4741 tagggtgtcg ggttaatcag cgtcagagaa ttcgcgctac gacgaaatct taatttttct
     4801 gcggcctgat cgggtggcaa cgctaattta gccgggcgat agtacagttt aatgcggctg
     4861 ataattgcga actgtagcgt attctcagtc aattttgatt tatccattga cggaatcgct
     4921 ttaacattca tccagaataa actttcccgg tcctgtggca attggttatt tgttgcatca
     4981 agaatacgca aggtattctc tttttttccc ttcatcgcaa acagaggagg cgtcacgata
     5041 aaacgaccat cctttacacc atcggcattt tccatccatg attgaattaa ataggtacta
     5101 ttttcatcat tatttgtcac ggcaagttgc acttgttttt gccctgccgg ataaattacg
     5161 cgagtcgcac ctaaggccac tcccgcttca gcgcgtccgg caaccatcat tgccatgaac
     5221 atcaggatac ctgccagcaa gcagaatgtt atttcctgcg atttccttac attggcgttt
     5281 ttattactca ctgtcctgtt cctgttatca cattatctgc tgaacaatta ctgataggtt
     5341 aaagagaacc aggcctgggc attggcgatg ccgccagtaa cccaacgccc ggtagcacga
     5401 tatttggcga tgaaatgtag cgaagtagag cctgagtaaa gccgtttcca gtttactgga
     5461 ggacgattaa tcggtacgag gtttccttca tcatcaaaca acgctacgcc aatattggtg
     5521 gctatccctg gcccctctcc cacggaaagc acatccggat ttttaccatc cgcgacaccg
     5581 tgaaacgcca cacccacacg ttcactcacc accgtgctac attcccgtaa atgaataaca
     5641 aaaggcaccg gtgcgctatc ttcccctacc gcatgaaacc ggttactgct gatttgcccc
     5701 atattgaccg tcatttgttt atcaccggct tcaatccggc aagtttccgc aataatgacg
     5761 ccctgaaatt gcatatttcc gccgggcagc gtagtattcc atttatttcc ggccagagca
     5821 aacattggca gcaacgacgc taatagaaat aatctcttcc ttttcatcgt tatttttatc
     5881 gcacaagggt gggcatccct gcccgtaatg acgtccctga acctgggtag gttattgata
     5941 ctgaaccttg aaggtcgcat ccgcattagc tgcgccaggg gttgcgacac cggttgcata
     6001 ataacgcgcc tggaacggaa tggtgttagt accgttattc agggttgttt gctcactgaa
     6061 tgtcgcacca tccagcgtca gcgcagcacc cgttctgtcc aggatctgta caccaacgtt
     6121 tgttgcgcta cccgcagctg aactctgcag agccagaacg ttggtatgac ccgcatcaat
     6181 cgccgtacct aaaaaggcaa ctgcggcttt agatgcaaca ttggtatcgc aatcattcag
     6241 ctgaatgtta aaaccgacag cagaactggt tgctccttcc tgtgccagcg atgcggtacg
     6301 aacctgtcct aactgaacga tttgatcaac agagcctgca tcaactgcgc aagcggcgtt
     6361 aacaacttcc cctttaaagt gaacggtccc accattaacc gtcgtggcag cggccagagc
     6421 cgctgtagaa ctgagggaca gagccgacag aacaacgatt gccagagttt taattttcat
     6481 gctgctttcc tttaaaaaaa ctatttctaa atcgacatgg gcagtcgttc tgtacacttt
     6541 gttttgtcaa cgagtttgac tgccaacact gcacagtttt cccccaaaag atgaaacatt
     6601 tggggccatt ttgactcata gaggaaagca tcgcgcacaa actttttcag tttatttgtt
     6661 ggcttaatat tctattgtta tctttattta tagattttta tattgcatga ggtggttttt
     6721 ggagagaaga atgaggaaga tgcgtcgagc cacagaaacg ttagctttac atatagcgga
     6781 ggtgatgtga aattaattta caagagaaat aatttacata tcaaacagtt agatgatttt
     6841 tgtcgttttt taaaattttt atgcttgaga aaaaatacgt aacttattta tgatatggac
     6901 aatttggccc caattgtctt gtatttattt tttaacttta ttatcaataa gttaattcaa
     6961 acctcttctc tttttaattt ttcgtttatg agattatttc tttcccataa tccggcaaaa
     7021 cgagcagcat tactggcggt ataacgtaca gtatggcgaa tatttcgatg cccgagataa
     7081 tcctgaatta aacgagtatc tgcaccacgc tccgccagtt cataaccgca agcatgcctt
     7141 aacatatgag gatgagtctg cgttacggtt ccagcttcaa taccggcatc acgaataatg
     7201 cgataggcct gctggcgaga aagccgactc ccgcggcgag aaataaatat ggcgtcggtc
     7261 cggtcagcgc ctttccagtt agcacgttcc agggtccagc gttccacagc ttcacgctca
     7321 tcaaaacgta acgggtgaac ggtagaaaat ccgttcttca gtcggcgaat atttattcga
     7381 ccttcattaa ggtcaagatc ctgataatgc agatcaagca gttcactaat acgcatccca
     7441 tgccgatatg ccaacagaat aagacaataa tctctggctc ccgttgcccc gtaacaaacc
     7501 gcctgcatca tggcctgaac ttctttaccg gtaagataac gacgtttact cacaatagta
     7561 gtactcctaa ctgagatata tttgaatgaa tacctatagg aaacctcaat cggtcaaaat
     7621 tagcccaata gataaaaaga taaccacaca cgaagtgatg tggctatcag tcaattacat
     7681 taaatatcat acaaaaataa aatatcactg atgtgacaac atctattttc attgatttga
     7741 ttcaatataa caatagattg tagtagatta gcttccagtt gcttaaaaaa taaagaaaaa
     7801 agtgcgaaaa caggagaatt taagtgatgt gtaaatttat gaatgatttt gtttatttat
     7861 tttgaagaac ataaaccatt gtattttgta tggatatggt taacttaatg cttattatga
     7921 taattaagaa ttatacttac aataacatca attaataaat ggttaatcat ttttgatata
     7981 tcgtaagaat aatgtagttt ttaacaccat ccctggtatc tcaactatct ctataaaaca
     8041 gcgtgacgct gtcgtcctct ggctctatcc cagatgccgt aaaaacgccc tgcattgctg
     8101 gcggtatacc agacagtatg acgaatattg cgatgcccaa gataatcctg gataagtcgc
     8161 gtatctattc ccatattcgc caaagcaaaa ccacacgaat ggcgtaacat gtgcggatga
     8221 atctccagtg acaacccggc attaccaccg gaagtcgaga taatatggta aaactgttgc
     8281 cgagaaagcg gattcccctt acgcgataaa aatacccact cgctctcagc atgcgggtac
     8341 gaagtacgga tactcaacca gttttttaaa gcctgaactt ctttattcaa tagcgggggc
     8401 gttgttgaaa agcctttttt taatcgatgg atatatatac actttgcctt aagatcaata
     8461 tccgaaatcc tcaatcgaca aatttcactc gcccggaaac catgaataaa acaaagcaaa
     8521 gtcagacaat aattacgtgc tgcatgaggc ccggtatttg ctgctttaag gagtgattcg
     8581 atttcactat gggtcaggaa gttccttttt ttgttatcag ccttattctt catcgttttt
     8641 cccttataat tacagacgcg cactagctgt gctgggttag caataatcca acatttttat
     8701 cctgattatg tttataaaag cgaacgtttg cttaataact aacgtaagtg gaccagttct
     8761 tctgagtgaa cttaaatggg gtagcaacag ttaattataa taacgttgcc atacaaacgc
     8821 tatgctcttg catgctatgt acctttatat atttatcaat cggatacgaa tctaataata
     8881 cccctcatca ttaatgcaac taatcttatc tatatatcat gtgatatgtt ctgtaacaag
     8941 taaaatcagc ataaaaaatt cagcaaaatt taatattcgt tcaactagcc gtctcacata
     9001 tgacattatt agtcaactcc atttgccaca aaatggctat ttcatggaac gcagtaattt
     9061 cttagtggct attatcatgc taattcttag gtccctagcg attattcctg gctgattcgt
     9121 cagcttacgc catgagattg ttacattcct ttttatcctg gagatatggg ggaacgcagg
     9181 agatgatttt ttcatctaca ttcgttggta aatatagctt tggtcactac gacttaaacc
     9241 ttcctcaatc acagccattc gccgggcgat acggcaaaca aactaagtga aacatcatcc
     9301 tgaaaacacc aacatcaaca agcctctcca gatcgacttc agaagtgacc agttacaagc
     9361 cacaaaccag gaaacatatt tcatgatgag aattatgctc agcctgatgg cgggtaaaca
     9421 ctgattttca ccaagctcac tgagttagct tgcaaagctt cccttgttta gactccaaat
     9481 aaattattta taaaacaaac atttaaacaa agatctttag tttttgaaga ccaccgcaag
     9541 tgttcgtctg gcttcacatg gcatcttcct cttagaaaaa gatcgacata ttttgtgaca
     9601 cgaattgcaa atctggtttt gttgtatgga ttgcgtgatt tttgatctgg tataacaggt
     9661 ataaaggtgc accaagatag tcaatgagac agggcatctc gcaatctatg gcaaacatca
     9721 cttcagttct ttctcatcgg gtgatgaaaa cgcacttcag tctgaaagga atatgaaaat
     9781 gagatcaaca gacactctat tttatgactc tgggtaaaat ggattgagta agtgatatag
     9841 cttacgaaaa ttcaaatcaa ttaaacatca gaagagattt tatactcagg tatttaatct
     9901 ggatctctgt ttatttaaat aatgtgaaaa gagatttttc acaggagacc ttatacaaaa
     9961 aaatataaaa tacagctacc ggttgccaaa gacactataa gcctggcaaa aaatattaca
    10021 caacataaat gctaattgtt tatgcgggct ttgtattgct ttctgtatcc tacaaatgag
    10081 tgaaatttat gaaaaaggct aaaatacttt ctggcgtatt attactgtgc ttttcgtccc
    10141 cattaatttc tcaggctgcg acactggacg tacgtggtgg atatcgtagt ggaagccacg
    10201 cctatgagac tcgactcaaa gtcagtgagg gatggcaaaa tggatggtgg gcaagcatgg
    10261 aaagtaatac ctggaatacc attcatgata ataaaaagga aaatgccgca ctcaatgatg
    10321 ttcaggttga agttaattac gcgattaaac ttgatgatca atggacggtg cgcccgggaa
    10381 tgttaacgca ttttagcagc aacggcacac gctacggacc ctacgtaaaa ctgtcctggg
    10441 acgcgacaaa agatcttaat tttggcattc gctatcgtta cgactggaaa gcttaccgac
    10501 aacaagactt atccagtgat atgtctcgtg ataacgttca tcgttgggat ggatatgtca
    10561 cttaccatat taatagtgat ttcaccttcg catggcaaac gacgctatac agcaaacaga
    10621 acgattatcg ctatgcaaac cataagaaat gggcgacgga aaatgcattt gttctacaat
    10681 accatatgac gcccgatatt acgccataca tagaatatga ctaccttgac cgtcagggtg
    10741 tttacaacgg cagagataat ttatcggaaa acagttatcg cattggtgtg tcatttaaac
    10801 tgtagtagac aggagacagt cacaatgaat aaaacaataa cggcgcttgc tatcatgatg
    10861 gcttcatttg ccgcaaacgc gtctgtatta ccggaaactc ctgtgccatt taaaagtggt
    10921 accggagcaa ttgataacga cactgtctac attggtttag gtagcgcagg tacggcatgg
    10981 tacaagctgg atacacaggc caaagataaa aaatggacag cgttagctgc attccctggt
    11041 ggaccaagag atcaagcaac ctctgcattt attgatggca atctgtatgt gtttggcggc
    11101 attggcaaaa acagagaggg cttgactcag gtatttaatg acgtacacaa atacaacccc
    11161 aaaaccaata gctgggttaa attgatgtcg cacgcaccga tgggcatggc gggtcatgtg
    11221 acttttgtac acaacggcaa ggcttatgtt actggcggtg ttaaccagaa tatcttcaat
    11281 ggctattttg aagatctcaa cgaggctgga aaagattcaa ccgctgtaga taaaatcaat
    11341 gcctactatt ttgacaaaaa agcagaagat tatttcttca ataagtttct gttgtctttt
    11401 gatccctcaa cacagcaatg gagttacgct ggcgaatcgc cctggtacgg aacggctggt
    11461 gcggcggttg tgaataaagg tgataaaacc tggcttatta atggcgaagc caaacccgga
    11521 ttgcgaacgg atgccgtatt tgaacttgat ttcaccggta ataatttaaa atggaataag
    11581 cttgctcccg tctcatcacc agatggcgtc gctggcggtt ttgcggggat aagcaatgat
    11641 tctcttatat ttgccggagg ggccggattc aaaggttcac gagaaaatta ccagcacgga
    11701 aagaactatg cgcatgaagg cctgaaaaaa tcatatagca ctgatattca tctttggcat
    11761 aacgggaaat gggataaatc gggtgaatta tcgcaaggtc gggcctacgg agtatcattg
    11821 ccctggaata atagtctatt gattattggc ggtgaaactg caggcggcaa agcggtgacg
    11881 gattcagttt tgatctctgt gaaggataat aaagtcacag tacaaaacta acgcttcagg
    11941 gccccagtaa aggggccctg ctatcaattt atcatttcaa ttacgcgaaa attatatgga
    12001 cgcaataata tcgcccgatt attactatgt tcttaccatt gctggtcagt ctaatgctat
    12061 ggcgtatggc gaaggactgc cattaccggc cagggaagat gcgcctcatc ccagaattaa
    12121 acaattagcg agatttgcgc atacgcatcc cggaggcccg tcatgtcact ttaacgacat
    12181 tattccacta actcactgcc cacacgatgt tcaggatatg cagggttatc accatcctct
    12241 ggcaacgaat catcaaacac agtacggcac cgttggccag gcactgcata ttgcacggaa
    12301 attactgtcc tttattcctg ataacgcagg ggttctcatc gttccgtgtt gccgtggcgg
    12361 atcggctttt accgcgggca gcgaagggac atattcagaa cggcacggag ccagccatga
    12421 tgcttgtcgt tggggaacgg atactccgct ataccaggat ttagtcagca gaacgcgagc
    12481 cgcactggca aaaaatccgc agaacaaatt cctcggcgta tgctggatgc aaggcgaatt
    12541 tgacttaatg accagtgact acgcgtcaca ccctcaacac tttaatcata tggttgaagc
    12601 ctttcgtagg gatctaaaac aataccattc tcagcttaat aatattactg acgcgccgtg
    12661 gttttgcggc gataccacct ggtactggaa agaaaatttc cctcatgcgt atgaagctat
    12721 ttatggcaat tatcaaaata atgttttagc taatattatt ttcgtcgact tccagcaaca
    12781 aggtgaaaga ggactgacga acgcgcctga tgaagacccg gacgatttaa tcatgggata
    12841 ttacggttca gcgtaccgct caccggagaa ctggacgacg gcactgcgaa gcagtcattt
    12901 cagcgcggca gcccgtcggg ggattatttc tgacaggttt gtagaagcaa ttttgcagtt
    12961 ttggcgcgga aagtgagcgt tcattaattt gatttattcg catccatcaa cgcttttaac
    13021 tcctccggcg taaacggtgg tttgcgtcgg tgcaacatcg catggcaatt agggcacaca
    13081 agcagcaaaa aaacaaatga gatcaaggta atatatcttc acttgcaggc ttgaacagca
    13141 gaaaatttgc gagcatgatc gcaattattt cactgctagg gatatcaatg gcttccagta
    13201 aatcgctaca gcaggcaatt gccaatataa aaatctggca taagggtgaa cagcgcgcac
    13261 cgcataaacc attgttattg ctatacgtat tagcgggata cctgaatgga catccacgcc
    13321 ttttcgatta tggatcggaa atctacgaac ctctgcacag tttactggaa cgttttgggc
    13381 cacagcgttc acagtaccga cctgatatgc cgttctggcg attacaaggc gacggattct
    13441 ggcaattaca caatgccgag ctttgctcaa cagcagggag cagccgacaa ccgccagtga
    13501 aggaactgaa cgaataccac gttgcaggcg gtttcgatga acagcactac gccctggtaa
    13561 ccggcaataa aaaactcatc aatactctag cgcaacagat actcgaagcg cacttcaccg
    13621 agagtattca ggaagaaatt gctgacgagc tgggttttga tctccagcaa atccgtaaac
    13681 agcgcgaccc gttatttcgc aaaaatgtat tacgcgctta taactaccag tgcgcgatat
    13741 gtggctttaa tatgcgccac gacgacacca ccgtcgcgct ggaagcggcg catataaaat
    13801 ggaaacagca cggcggtcct tgtgaaattc ctaatggcct ggcgctttgc gcaattcacc
    13861 ataaagcctt tgataaagga tcgattggcc tggacgaaaa tatgcgggta ctggtatcag
    13921 atgcagtaaa cggcggaggg agcgttgaga gattattctg ggattttgac gggaaaatta
    13981 ttgcgttgcc gcaggtgcgt aaaaattacc cttatgaggg gtttgtggag tggcatcgga
    14041 atgaggtttt tagggggtga ggttgataac tatcgcagta tgttcaatat tgctgacata
    14101 atcagtgcag gatcttgccg ctagtttaca actcgccttc gatagcggcg caaaacgagt
    14161 tctgttgccg atatcctggg ctatggatat tccaacagtt ccggcagagt tgtttaccaa
    14221 gtttcaggta agtttttact cagaaccggt tgatgcggtt tataaggcgc tggatgtgaa
    14281 ttaacacatt cctcagctta tttacaaaag gattattccc atttgatata atatcagtgc
    14341 caatttactt ttttcaatgg aatatatctc ggctaaataa caataaaact tatcgctaat
    14401 ggattaaata aacaattttc taattcattt cacggtaatg actccaactt actgatagtg
    14461 ttttatgttc agataatgcc cgatgacctt gtcatgcagc tc
//
LOCUS       DAJVUJ010000102        14331 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.102, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000102 DAJVUJ010000000
VERSION     DAJVUJ010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14331
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.102"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(<1..609)
                     /locus_tag="OQQ48_002782"
     CDS             complement(<1..609)
                     /locus_tag="OQQ48_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HCS1953490.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFK"
     gene            complement(997..1743)
                     /gene="cutC"
                     /locus_tag="OQQ48_002783"
     CDS             complement(997..1743)
                     /gene="cutC"
                     /locus_tag="OQQ48_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HCS1953491.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQWVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLNTLSNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DAHADEYSRYVVDGAAVAEMKGIIERHQAK"
     gene            complement(1757..2323)
                     /locus_tag="OQQ48_002784"
     CDS             complement(1757..2323)
                     /locus_tag="OQQ48_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCS1953492.1"
                     /translation="MANWQSIDELQDIASDLPRFTHVLDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEYERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            2539..4272
                     /gene="argS"
                     /locus_tag="OQQ48_002785"
     CDS             2539..4272
                     /gene="argS"
                     /locus_tag="OQQ48_002785"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCS1953493.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVSTPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWHKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            4449..4937
                     /locus_tag="OQQ48_002786"
     CDS             4449..4937
                     /locus_tag="OQQ48_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HCS1953494.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDISAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGVKKPDIEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(5057..5449)
                     /gene="flhe"
                     /locus_tag="OQQ48_002787"
     CDS             complement(5057..5449)
                     /gene="flhe"
                     /locus_tag="OQQ48_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HCS1953495.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPSGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(5449..7527)
                     /gene="flhA"
                     /locus_tag="OQQ48_002788"
     CDS             complement(5449..7527)
                     /gene="flhA"
                     /locus_tag="OQQ48_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HCS1953496.1"
                     /translation="MSNLAAMLRLPANLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGCEQKA
                     PAEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(7520..8623)
                     /gene="flhB"
                     /locus_tag="OQQ48_002789"
                     /pseudo
     CDS             complement(7520..8623)
                     /gene="flhB"
                     /locus_tag="OQQ48_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
     gene            complement(8706..9350)
                     /gene="cheZ"
                     /locus_tag="OQQ48_002790"
     CDS             complement(8706..9350)
                     /gene="cheZ"
                     /locus_tag="OQQ48_002790"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HCS1953497.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(9361..9750)
                     /gene="cheY"
                     /locus_tag="OQQ48_002791"
     CDS             complement(9361..9750)
                     /gene="cheY"
                     /locus_tag="OQQ48_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HCS1953498.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFDKLDM"
     gene            complement(9765..10703)
                     /locus_tag="OQQ48_002792"
     CDS             complement(9765..10703)
                     /locus_tag="OQQ48_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HCS1953499.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPVGMLAMRQAGAWTLAQNEASCVVFG
                     MPREAINMGGVCEVVDLSQVSQQMLAKISAGQAIRI"
     gene            complement(10706..11518)
                     /gene="cheR"
                     /locus_tag="OQQ48_002793"
     CDS             complement(10706..11518)
                     /gene="cheR"
                     /locus_tag="OQQ48_002793"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005063150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HCS1953500.1"
                     /translation="MRLPGCSRYSFRRISQLIYQRAGIVLADHKRDMVYNRLVRRLRS
                     LGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHHFPLLADHARRRSGEYR
                     VWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVLEKARSGIYRHEELKNL
                     TPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQYTVPGPFDAIFCRNVMI
                     YFDQNTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLRGQTVYALSKD"
     gene            complement(11656..12222)
                     /gene="flhC"
                     /locus_tag="OQQ48_002794"
     CDS             complement(11656..12222)
                     /gene="flhC"
                     /locus_tag="OQQ48_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HCS1953501.1"
                     /translation="MSEKSIVQEARDIELITLGARLQMLESETQLSRGRLIKLYKELR
                     GSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLYLEQC
                     PQAEEGPLLALTRAWTLGRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLCQPPS
                     RAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(12225..12575)
                     /gene="flhD"
                     /locus_tag="OQQ48_002795"
     CDS             complement(12225..12575)
                     /gene="flhD"
                     /locus_tag="OQQ48_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HCS1953502.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVNDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
     gene            13354..13782
                     /gene="uspC"
                     /locus_tag="OQQ48_002796"
     CDS             13354..13782
                     /gene="uspC"
                     /locus_tag="OQQ48_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HCS1953503.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
     gene            complement(13789..>14331)
                     /locus_tag="OQQ48_002797"
     CDS             complement(13789..>14331)
                     /locus_tag="OQQ48_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HCS1953504.1"
                     /translation="QIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLYYLNQ
                     HFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAANELT
                     SALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISDLKQI
                     VPRSAESQQRDKVATFPKLA"
BASE COUNT         3685 a         3784 c         3342 g         3520 t
ORIGIN      
        1 tttgaaagga taaatatgtg cgccattagt cactgaagta gcggcctgac tttccagctc
       61 atgcgccgcg ttgtcatcca tttttatttc tggcggaaaa tgggcaatgc ctttatgaca
      121 atcgatacag gtttcgccgc ccttttgtgc tttgttatgc attttctgcg cagattcact
      181 ttgcgaggca atatccatgg catcaaaaga atggcaacta cggcacgttg cagagtcagt
      241 tgcttttaat tctttccata ctgtttcggc catttcctgg cgatgagttt cgaacttatc
      301 gtcactgtct attttgccgc taacaaattc atgataaata tctttagatg cttttaattt
      361 agcaaataaa taatccatcc ctgactttgg aatatggcaa tctgcacatt ccgcacgtat
      421 ccctttctgg ttcgaaaagt ggacagttcc ctgatattcc tcaaaaggtt tactcatcga
      481 gtggcaggaa aggcaaaatg ctgtatccga cgttttatgt aaggcttttt gcgccagcaa
      541 taacccgccg ccaccaacca caaccgctat cagcaaaaac aataacccaa tgcgtttttt
      601 ccctcgcata gattcctcta aataaattcc agaacaccga aatcattgga taattaaata
      661 cacaacgaat taaccgcttg cataactcgg cacaacactg cctgaaataa tcgataaaga
      721 atatgattta ttacaatgta atcattaatt gctaaggaat aaccctgttg ttatttagaa
      781 tatatatttg tcagttttag caatttaaac cccataatta atgtgaatat ataaaatatt
      841 tttagcacaa cgctatgttc actgtcaccc gctcaacaga tcacggttat cgtacgtttt
      901 ttatactgct cagggataaa aaaaggcccc tgtcgaaatt gcaggggcct ggtacgagca
      961 agcatcatat tgggcgacat gatgcaacgg taaaaatcat ttggcctgat ggcgttcaat
     1021 gattcctttc atttcagcaa ccgccgcccc gtctacgaca taacgcgaat actcgtccgc
     1081 gtgtgcatct gatgacatgg acaatccttg attacgataa cgcatcggtg aagcttgcca
     1141 cgctcccgcg gagctatgga cttccagcac tccggcatcg aggaagtggt gcaggttttc
     1201 tgcacggact cctgctccgg ccataatgat tggagcatca cgatgggcaa taagttccat
     1261 aatttttgat aaaccttgca gtgcatctga tttttgccct gatgtcagca cccgggcgat
     1321 gcccaattcc gcaagattac tgagtgtatt taaagggtta gcgcacatat cgaaggcgcg
     1381 atgaaaagtc actgccagcg gaccggcagc agccattatt ttttccattc gtggcatatc
     1441 gacattcccg tcaacatcga gcacgcccgt caccagtcca ggaaaaccta attcgcggac
     1501 tgtgcgcaca tcctcaagaa tggcggcaaa ctcaccgtcg ctgtagcaaa aatcaccacc
     1561 gcgtgggcgg ataatcggat gcacagggat cgtcacccac tggcgcacgg atttcagtac
     1621 acccaacgac ggcgttaagc ccccctcttt tggggctgcg cataattcaa ctctgtctgc
     1681 gccgttttgc tgcgccgtta gtgcacattc catgctgtaa cagcaaattt ccagtaatgc
     1741 catttttact ccttaattac gccgactgct cgctggcaac gatctcttca atggaccacg
     1801 gatgaaactt aatagttgtt ttgccatcgg tgaccgccag cgtcggattc ggtaatcgct
     1861 catactcacc ttttggcgaa ctggttttca ctgagatccc cggtaggctg agtccttcat
     1921 cagaaagcaa tgccagcgca cgggcattca gcgtttctgg atcacccggc agaacgattt
     1981 caatatgttc ccaaccttcg tgtgggtaac gtttttcccc gggccacggt agctccacaa
     2041 tggaaaactg ccagtgcgca acctgtaccg gttcatgcaa tttaaacaga caaatcggtc
     2101 tgccattgat catattttct gacaaaagct cgccacactg ttcaaaaccg cgacgccagc
     2161 gctcagcggt gacgttttga tggcaacgca aagaaatgtg atcggcagtc agcggagtga
     2221 tatccaaccc cagacggcgg gagagttcat ctaatacgtg ggtaaatcgc ggtaaatccg
     2281 atgcaatatc ctgcagctcg tcgatagatt gccagttcgc cataatcact cttcgtcttt
     2341 cagtaaaagc gttaatttac cctgttgccc tgcgccaacc aaccgctgat ttcacgccgc
     2401 ttctgatgca atagtgaaaa cggcaatacg ccacgcgcac gttgctgacg aaaacagcca
     2461 tttgcagtat actcccgccc taatttcttt aactggtgcg ggcaattttt gctcgcttca
     2521 tcaatgtaag gtattccggt gaatattcag gctcttctct cagaaaaagt ccgtcaggcc
     2581 atgattgcgg caggcgcgcc tgcggattgc gaaccgcagg ttcgtcagtc agcaaaagtt
     2641 cagttcggcg actatcaggc taacggcatg atggcagttg ctaaaaaact gggtatggca
     2701 ccgcgacaat tagcagagca ggtgctgact catctggatc ttaacggtat cgccagcaaa
     2761 gttgagatcg ccggtccagg ctttatcaac attttccttg atccggcatt cctggctgaa
     2821 catgttcagc aggcgctggc gtccgatcgt ctcggtgttt ctacgccaga aaaacagacc
     2881 attgtggttg actactctgc gccaaacgtg gcgaaagaga tgcatgtcgg tcacctgcgc
     2941 tctaccatta ttggtgacgc agcagtgcgt actctggagt tcctcggtca caaagtgatt
     3001 cgcgcaaacc acgtcggcga ctggggcact cagttcggta tgctgattgc atggctggaa
     3061 aagcagcagc aggaaaacgc cggtgaaatg gagctggctg accttgaagg tttctaccgc
     3121 gatgcgaaaa agcattacga cgaagatgaa gagtttgccg agcgtgcacg taactacgtg
     3181 gtaaaactgc aaagcggtga cgaatatttc cgcgagatgt ggcacaaact ggtcgacatc
     3241 accatgacgc agaaccagat cacctacgat cgtctcaacg tgacgctgac ccgtgatgac
     3301 gtgatgggcg aaagcctcta caacccaatg ctgccaggaa ttgtggcgga tctcaaagcc
     3361 aaaggtctgg cagtagaaag cgaaggggcg accgtcgtat tccttgatga gtttaaaaac
     3421 aaggaaggcg aaccgatggg cgtgatcatt cagaagaaag atggcggcta tctctacacc
     3481 accactgata tcgcctgtgc gaaatatcgt tatgaaacac tgcatgccga tcgcgtgctg
     3541 tattacatcg actcccgtca gcatcaacac ctgatgcagg catgggcgat cgtccgtaaa
     3601 gcaggctatg taccggaatc cgtaccgctg gaacaccaca tgttcggcat gatgctgggt
     3661 aaagacggca aaccgttcaa aacccgcgcg ggtggtacag tgaaactggc cgatctgctg
     3721 gatgaagcct tggaacgtgc acgccgtctg gtggcagaaa agaacccgga tatgccagct
     3781 gacgagctgg aaaaactggc taacgcggtt ggtattggag cggtgaaata tgcggatctc
     3841 tccaaaaacc gcaccacgga ctacatcttc gactgggaca acatgctggc gtttgagggt
     3901 aataccgcgc catacatgca gtatgcttac acgcgtgtat tgtccgtgtt ccgtaaggca
     3961 gaaattaacg aagagcaact ggctgcagct ccggtaatca tccgtgaaga tcgtgaagcg
     4021 caactggcag ctcgcctgct gcagtttgaa gaaaccctca ccgtggttgc ccgtgaaggc
     4081 acgccgcatg tgatgtgtgc ttacctgtac gatctggctg gtctgttctc tggcttctac
     4141 gagcactgcc cgatcctcag cgcagaaaac gaagaagtgc gtaacagccg tctgaaactg
     4201 gcacaactga cagcgaagac gctaaagctg ggtctggata cgctgggtat tgagacagta
     4261 gagcgtatgt aatcgatttt tcgtgagagt aaagcctgat cagggttacc tgatcaggct
     4321 tttttattgc catctaaatg tattctgaaa atggacatgc cattgttttc tgactgttgg
     4381 ataagaggcc agaagcgtaa tatccggccc cagggaaacg ataacggttg aatttaagga
     4441 ataccgcagt gtttaaattt cttgtattaa cattaggcat tatctcttgc caggcttacg
     4501 cagaagatac ggttatagta aacgaccatg acatttcagc catcaaagat tgttggcaaa
     4561 aaaattcaga tgatgatact gacattaacg tgatcaaatc atgcctgcga caagaataca
     4621 atctcgtcga tgcgcaatta aataaagcct atggtgaagc ttatcgttat atagaacaag
     4681 tgccacgcac aggtgtaaaa aaacctgata tcgaacaact taacttgctt aaaaaatcac
     4741 agcgagcctg gctggatttt agggacaaag aatgtgaatt aatcctttca aatgaggacg
     4801 ttcaggattt aagtgaccct tattctgaat cagaatggct ctcatgtatg atcatacaga
     4861 ccaatacgcg aactcgccag ttgcagctat accgtaactc tgaagatttt tatccaagcc
     4921 ctttgacaag aggataattc acatcttttt ggcatgtttt gttgcaagct attcctgata
     4981 aataattgca acaagacatc gagccttttt cactgagtta ttaaacatac tcgcgagcgc
     5041 gtaatttttt tgtccttcag cgataattca caatcacttc attacgttgt acctttagcg
     5101 gtggaattaa ccgcccaccg cctggcactt cccagataaa tcgcaacggt tctgctgccg
     5161 ctataccgga aaaggccacg gtggttccgc tctgcccctc taattcgaca caacgagatt
     5221 gcgaacacaa gcgaacccgc agtcctgaag gtgtcgggcc gataagctga taacgccacg
     5281 ctaccagcgt catcaatcct gaagcaggtt gtcgcgtaga aagaggcgca gacgacatcg
     5341 actcaccgcg atgatttagc gtaattccta cactacttgc ctgccacatc ccctccccgg
     5401 cggcttgcac cagcagcgga aacaataata ttgctaataa ggttctcatt atttgccgcc
     5461 aattgtcgcc gtcatgcgga tatgtcggtt atcagacagt tccagattcg acaggaccac
     5521 taactgcggc aagctgcggc gcaggaagcg agacaataat ggtcgcagcg cgtggttcac
     5581 caacaatact ggcggcgcac ccagcatctc ctgacgggat agcgcttcct gagtttgcgc
     5641 cagtaaacga tccgccagcc ctggctccag tcctcccccg ccctgcagcg cctgtagtaa
     5701 caaacgttcc agcggtgtat cgaggccaat aacatggact tcatctttgc caggaaacca
     5761 ctgctgggta atcgcccgtc ccaacgccac gcgcacgacg gcggttaatt catgtggatc
     5821 gctttggatg ggcgcatgtt ccgccagcgt ttcgagaatg gtgcgcatat cgcgaatcgg
     5881 tactttttca tcgaggagat tttgcagcac tttatgcagt gtggtgagcg tgacgacgcc
     5941 aggaacgaga tcttccgtca gctttggcat ctcctgggcg acgcgatcca acagctgttg
     6001 cgcctcctga cgaccaaaca gctctgcggc atgctggcta atgaggtggt taagatgcgt
     6061 tgctaccacc gtgctggcct caaccactgt gtacccctga atctgcgcct gttcttttag
     6121 cgcactttca atccagatag cattcaggcc aaatgccgga tcgacggtcg cctcaccagg
     6181 taacgtcccg gcagcggttc cagggttaat cgccaaccag cgccccggat aagcatcacc
     6241 actgccaatc tccacgcctt tcatcaaaat gcgatagcgg gcaggttgca gatccatatt
     6301 gtcgcgaatg tgcaccactg gcggcagaaa tcccatctcc tgggcaaatt tcttgcggat
     6361 actgcgtata cggcccaaca actcaccatc ctgctggaaa tcgaccatcg ggatcagtcg
     6421 ataacccact tccattccca gagaatcttc cagttgtaca tcgttccacg tcgcttcgac
     6481 aacggtatta ttctctgcca tttttaccgg tttgggttcg gcaggcgctt tttgttcgca
     6541 tccgcgtatc caccaggcca gccccagcaa tccggcagtg aacagcaaaa ataccaggtt
     6601 cggcattcca ggcaccaagc cgagtaaacc gagcacggcg gcgcttaaca acataacgct
     6661 tgggttactg aaaagctgat tcaccatctg ctcgccaaca tcctgatcgg tgctgacacg
     6721 cgtaacgatg acccccgcgg cggtagaaat caccagcgcc ggaatttgtg ccaccagacc
     6781 gtcgccaatg gtcaatagcg tataactttc cgccgcgtgt cccatgctca tgccatgttg
     6841 cagcacgccg accagcaacc cgccgacaac gttaatgacc atgatgagga tcccggcgat
     6901 ggcatcgccg cgaacaaact tacttgcccc gtccattgag ccgtaaaaat cggcttcctg
     6961 agtcacttcg gagcggcgtt ttttcgcctc atcttcacca atcaatccag cgttaaggtc
     7021 ggcgtcaatc gccatctgct tacccggcat accatcgaga acaaagcgcg cacccacttc
     7081 tgcgatacgc ccggcacctt tggtaatgac cataaagttg atgatcacga gaatgacaaa
     7141 caccacgata cctatagcga aattgccacc aacgaggaag tgaccgaacg cttcgaccac
     7201 cttccctgcc gccgccgcgc cggtatgccc ttccatcaaa atgatacggg ttgaagccac
     7261 gttaagtgcc agacgcaaca acgtagtaaa caacagaatg gtcggaaacg cagcaaactc
     7321 aagcgtgcgc tgggtaaaca tcgccaccag caacaccatg atcgacaagg caatattgaa
     7381 ggtaaacaac aggtcgagta tgaatgcggg cagtggcagc accatcatcg acaagatcaa
     7441 caggatcaaa atcggtccgg caaggatctg ccattgtgtc gatttcaggt ttgcgggcag
     7501 gcgcagcatc gcggccagat tactcatggg tcggtttctc gttaataaaa tccagggctt
     7561 ccggcaccgg aagatgagta ggttgtacag ggcgctgtcc accagccaga cgccagcgtt
     7621 tcagttgcca gatccaggcc agcacttccg ccaccgcggc gtacagttga cccgggattt
     7681 gttgaccaat ctccgcatgt cgatacagcg ctcgcgccag cggcggcgct tcaagcgtcg
     7741 ggacgttatt ttcagcgcca atttcacgaa tgcgcagcgc gaccagccct gcacctttag
     7801 cgaccacttt cggtgcgctc attttgtttt cgtcatactg caacgctacc gaatagtggg
     7861 tcgggttatt gacaatgaca tccgctttcg gcacatcggc catcatccga cgccgtgcgg
     7921 cagctcgctg catctgacgg atccgtcctt taacatgggg gtcgccttcg ctttgtttga
     7981 actcatcacg aatatcctgc cgcgacattc gcagcttttt caggtggctg aagatttgga
     8041 aaaagacgtc aaatcccacc attggaatga caccaagcac caccagcagt gcgcatagcc
     8101 ctaccaaatc catcgcatta cccatggcgg taatcggaga ctcggccagt aagcgcatca
     8161 tctgcggcca gtgatgccag agaaaaaacc ccgtcacgct gccaaccaag atggttttca
     8221 aaattgcttt aagcaactcc gcgccagtct gtgccgagaa catccgttta atgcccggta
     8281 gcgggttgag tttggaaaac ttcggctgca aggattttcc gctaaatacc agccccccca
     8341 gcatgaccgg agaaatgagc gctaccagca ccacgccgct aatcagcggt agcagcgcca
     8401 gcatggcttc tctgatcagc agaataatct gcccgaggat cagattcggg tctttgataa
     8461 tactgtgatc aaaatgcagc ccagcggaga gcatgcccga caatcgacgg gccagcgaca
     8521 caccgccgaa ccagataaca cacacgccca ccagcaaaat cagcagtgag gtcagttcac
     8581 gggaacgcgg gatttagggc ttttgctgcc acacatcaag catagtgtgc gtttgtcgga
     8641 tgcggcgtca tcgccttatc agaccgcctg atatgacgtg gtcacgccac atcaggcaat
     8701 acaaatcaaa atccaagact atccaacaaa tcgtccacct gatcctgact ggctaccaca
     8761 ccggctttgc tggtatcgac ctgaggtcca ttaagcaaac tctggttttc acgttttgga
     8821 cgcgactcct gttccgggat gttttccaac agcaccatca gcaactggcg ttcgatctcc
     8881 tgaatgacat ccatcatccg cttaataacc tgcccggtga gatcctgaaa atcctgcgcc
     8941 atcatgattt ccagcagttg cgcgttagta aagctggtat gcgcgggtac atctgccaga
     9001 aattgtcgtg tatctgttac cagttcacgg gcgtcggcaa ggtcaatcgg atcggcaaac
     9061 cagtcatccc aacgttgggt taacgctttt gctgatttct ccatttgatc ctgatgcggt
     9121 tgtgacgcct caacactgtt cagcgcccgc tccgcagcct gggcggtcat ctgcacaaca
     9181 tagtacaaac gatcgcgcgc atcggggatg gcttccgccg cttcggcaat ggcctgatcc
     9241 agccccagtt cccgcaaact gtcgcgcagc atacgcgtca ggctgccgat gcgcgcaatg
     9301 atatcgccag ctgaatgctc gtcagcaggt ttgattgatg gttgcatcat agtcgcatcc
     9361 tcacatgtcc agtttatcaa agattttgtt gagtttttcc tccagcgtcg cggcggtaaa
     9421 tggcttcacc acatagccac tggcccccgc ttgcgccgca gcaatgatgt tctctttctt
     9481 cgcttctgca gtcaccatta acactggcaa tgccgacatc gcgccatccg cacgaattgt
     9541 tttcagcaat tccaggccat ccatattggg catgttccag tcggagataa caaatccata
     9601 accgcctgcc tgcaacttat tgagagcgtc gacgccatct tccgcttcct caacattatt
     9661 gaatcccagc tctttcagca ggttacgcac tatgcgtcgc atggtggaaa agtcatccac
     9721 aaccaaaaat ttaagttctt tatccgccat ttcacactcc tgatttaaat acgtatcgcc
     9781 tgtccggcac taatttttgc caacatttgc tggcttacct ggctaagatc gaccacttcg
     9841 cagacaccac ccatattgat ggcctcgcgc ggcatgccga acaccacgca acttgcttcg
     9901 ttttgcgcaa gggtccatgc ccccgcctga cgcatcgcca acattcccac cgggccgtcg
     9961 tgaattttga tttgataatt tgcgccacta cgcgccagtt ccatatgccg atcgcccggc
    10021 gcaatatagg catgccccgg caagacacgt tctccgtctt cggcttcttt aaccccgatc
    10081 tggcaaagct tattaagtct gtcggcaaaa gagcgggtga aaccgggcgg catatgttgg
    10141 gtaattaaca atgccgggct ggaaagcggc aacggttgca gtacgtgacg aattgcctca
    10201 gttccacccg ttgaagcacc aatcgcaatc agtttttcag aactcaacaa cggacccgcc
    10261 ttcagcgttg tcggtgctga caatggctta tgtgctgcaa ggctcgcctt tgctgccgta
    10321 cgcacctttt cagcaatcat ttcgctatac gccagcattc cttcgcgaat acccagttgc
    10381 ggtttggtga caaaatctat tgcccccagc tccagcgcgc gcagcgtgac ttctgatcct
    10441 ttgccggtca aggaagaaac catcacaacg ggcattggac gcaaacgcat taatttttcg
    10501 aggaaatcca gtccgtccat ccgcggcatt tcaacatcca gcgtcagcac atcgggattg
    10561 aatttcttaa tcaagtcacg cgcgaccagc ggatcaggcg cggtcgccac catttccatg
    10621 tcgctatggc tgttgatgat ttctgtcatg atctggcgca tcagtgccga atcatcgaca
    10681 gataacaccc tgattttgct catcgttaat ccttacttag cgcatacacc gtctgaccac
    10741 gcagcgtgaa gcggcgctca aggtggctaa agttttcaga gtgacccgca aacagcaatc
    10801 cgtcgggttt aaggagcgga acaaagcggc gcaaaatctc ctgctgggta ttttgatcga
    10861 agtagatcat gacgttacga cagaagatcg catcaaacgg ccccggcacg gtgtactgtt
    10921 tcgccagtag attcagcggg gcaaaatcaa catagttcgc cagctcctga cgcacgcgta
    10981 ccagcccttc atgcggtccc gtccctcgca tgaaataccg ttgcagttgc tgcggcgtca
    11041 ggtttttcaa ctcttcatgg cgataaatac cgcttctggc tttttccagc acttcggtgt
    11101 cgatatcact ggcaaacact ttccagcgtc cgggcgcggt gcccaatgtg tcagccagcg
    11161 tcatcgcaat gctgtacggc tcttcgccgg ttgaagccgc cgcgctccat acgcgatact
    11221 cgccagaacg gcgacgtgcg tgatccgcga gcagagggaa atgatgtgcc tcacggaaaa
    11281 atgccgtcag attcgtggtc agcgaattga taaacgcctg ccactcaccg ctgtgctgat
    11341 tagattccag caagttcaga taatgaccga aatccgtcag tcccagcgaa cgcaaacgac
    11401 gaaccagtcg gttgtaaacc atgtcgcgtt tatgatcggc caggacgatc ccggctcgtt
    11461 gatagatcaa ttgacttatc cgccgaaaac tgtaccgaga acaaccaggt aacctaataa
    11521 gataagcacg acatcatcct tccactgttg accatgacag gatgttcagt cgtcaggcgt
    11581 taacgcgcga ttggggcaaa aaaaagcagc ggtacgtcgt taccgctgct ggcatgttgc
    11641 gccacaccgt atcagttaaa cagcctgtac tctctgttca tccagcagtt gtgggataat
    11701 atcggcagga ttctgggaaa gtttacgtct ttttactgcc cgggatggcg gttgacataa
    11761 gctgcaggca aagctgccaa caggctggtg agcgtgggta ataaaattgc cgccgcagca
    11821 gttgcagctg gaaagttgca gtaatccact ttcaacaaac cgccccaatg tccaggcacg
    11881 ggttaatgcc agcagtggtc cttcttctgc ttgtgggcac tgttcaaggt ataaacggta
    11941 ggctttgatc accgcatcga cgccattaca caaacctgtt ttcagtaaaa actgccatgc
    12001 attacagaac atcgaagcat gaacgttttg ttcccaggtc ataaaccagt cggttgagaa
    12061 tggcagcatg cctttcggcg gtgggcttcc gcgcagttct ttataaagtt ttatcaggcg
    12121 tccgcgactt aactgtgttt cgctttccag catctgcaaa cgagcgccca gggtgatcaa
    12181 ttcaatatcc cgcgcttcct gaacaatgct tttttcactc atgatcaggc ccttttcttg
    12241 cgcagcgctt cttcaggctg attaacatca ttcagcaagc gtgttgagag catgatgccg
    12301 gtatgaattt gctggagatc gttaacgcgg gaatcttgcg tcaactgagt aatcgtctgg
    12361 tggctgtcaa aacggaagtg acaaaccagt tgattggttt ctgccagctt aaccatttgc
    12421 ggaagagtca gtgccgctaa cgttgtcgcc atttcttcat ttatgccgag acgaaacata
    12481 gcggacgctt tgtcctgaac aatcaaacgc tgtgcaagta gtaaatatga caagttgatg
    12541 tcataaatgt gtttcagcaa ctcggaggta tgcattattc ccacccagaa taaccaactt
    12601 tatttttatg cggtttcacc gcaccccgtg atgtcgccgg gaagccccgg taaaaaataa
    12661 ttagcattag aatagtgcga taagctgcaa taagcagaac cacctttttg gtttaatatg
    12721 tccttacaaa tagaaatggg tctttacact tatctaagat ttttcctaaa tcgacgcaac
    12781 tgtactcgtc actacacgca catacaacgg aggggggctg cgattttcaa taatgtgtga
    12841 tgcagatcac acaaaacact caattactta acataaatgt ataagccata cttttgtttt
    12901 gggtgtattt caatctgtta aaaagttttt cgctacgcta gcaagcaaaa atgaaactgg
    12961 aataatcgaa atgggatgtt gcgcacagtt aaaataactc accgtaaata atcatctgct
    13021 ataaataatc actttcatgc aataccagat aagctatttt taaacagaca cttaccgcac
    13081 aacaaactaa ttaaatacaa agaaaggcaa caatcaactt aatgttaatg aaatgtttta
    13141 catatattaa ccacttgttt aatgcggaga atcagtacac tattttctta taattacatt
    13201 tgaaattata tgatcaccgg gagtgattga ttaattgtct ttactaataa tcagactaat
    13261 atttacctgt ttgaccgagt tgggattgcg tcgtttctcc attaggagta aagctttaat
    13321 gtcacctgaa gttcacagaa taaggaacag gttatgagct atagcaatat tcttgtcgct
    13381 gttgcggtta caccggaaag tcagcaactg ctggcaaaag cggtatctat cgccaggcca
    13441 gtaaagggac acatcagttt aattactctc gcttccgacc cggaaatgta caatcaatta
    13501 gccgcgccga tgcttgaaga tttacgcagc gtgatgcaag aagaaacgca aagctttctt
    13561 gataagttaa ttcaggatgc ggggtatccc gttgacaaga cgtttattgc ctacggtgag
    13621 ttaagcgaac atattctgga agtatgtcac aaacatcatt tcgatttggt gatttgcggt
    13681 aatcacaatc acagtttctt ttcgcgagca tcctgctcgg cgaaaagagt gattgcctca
    13741 agtgaggtcg atgtgctgtt agttccactt acgggtgatt aaccgctcct acgcaagctt
    13801 tggaaaggta gcaactttat cgcgctgctg gctttccgcg cttcgcggaa ctatctgctt
    13861 taggtcgcta atgaagcact cctgccagtg gttaatatcg tttttcacga taacgtccag
    13921 catttctgca tgacgggaaa tacgttccgc cagcgacata gtcaatgcac gatccagcgc
    13981 agctgcaact tcgtcacgat cgtaggggtt aacaattaac gccgacgtta actcgtttgc
    14041 cgctcccgca aattgcgaaa gaacaagaac gcccgggttg gctgggtcct gagcagcaac
    14101 atactctttt gctaccaggt tcatcccgtc acgcagtggc gtcactaagc ccacgtcaga
    14161 gtagcggaat attttcatca gtaatttacg gtcaaaatgc tgattcaaat aataaagcgg
    14221 cgtccagcct aattgcccgt atttaccatt aattcgtcca gcttcatttt cgagctgatg
    14281 acgaatatcc tgataggctt gcacatcacc acgcgacgtt ggtgcaatct g
//
LOCUS       DAJVUJ010000103        14225 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.103, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000103 DAJVUJ010000000
VERSION     DAJVUJ010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14225
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.103"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            505..1329
                     /gene="gadX"
                     /locus_tag="OQQ48_002798"
     CDS             505..1329
                     /gene="gadX"
                     /locus_tag="OQQ48_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HCS1953505.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL"
     gene            1697..2425
                     /gene="gadW"
                     /locus_tag="OQQ48_002799"
     CDS             1697..2425
                     /gene="gadW"
                     /locus_tag="OQQ48_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HCS1953506.1"
                     /translation="MTHVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSLGTFS"
     gene            complement(2788..5901)
                     /locus_tag="OQQ48_002800"
     CDS             complement(2788..5901)
                     /locus_tag="OQQ48_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HCS1953507.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIGAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(5926..7083)
                     /gene="mdtE"
                     /locus_tag="OQQ48_002801"
     CDS             complement(5926..7083)
                     /gene="mdtE"
                     /locus_tag="OQQ48_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HCS1953508.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPG
                     SVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIE
                     ASKAIGDQWVVTSGLQAGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            7143..7421
                     /locus_tag="OQQ48_002802"
     CDS             7143..7421
                     /locus_tag="OQQ48_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1953509.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(7422..7949)
                     /gene="gadE"
                     /locus_tag="OQQ48_002803"
     CDS             complement(7422..7949)
                     /gene="gadE"
                     /locus_tag="OQQ48_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HCS1953510.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITLTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(8748..9320)
                     /gene="hdeD"
                     /locus_tag="OQQ48_002804"
     CDS             complement(8748..9320)
                     /gene="hdeD"
                     /locus_tag="OQQ48_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HCS1953511.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            9575..9907
                     /gene="hdeA"
                     /locus_tag="OQQ48_002805"
     CDS             9575..9907
                     /gene="hdeA"
                     /locus_tag="OQQ48_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HCS1953512.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            10023..10349
                     /gene="hdeB"
                     /locus_tag="OQQ48_002806"
     CDS             10023..10349
                     /gene="hdeB"
                     /locus_tag="OQQ48_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HCS1953513.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            10413..11060
                     /locus_tag="OQQ48_002807"
                     /pseudo
     CDS             10413..11060
                     /locus_tag="OQQ48_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
     gene            complement(11102..11632)
                     /locus_tag="OQQ48_002808"
     CDS             complement(11102..11632)
                     /locus_tag="OQQ48_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS1953514.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDGMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIIFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEAAVSLSRKQHQVLSCIANQMTTEDILEKLKITLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(11788..12354)
                     /gene="slp"
                     /locus_tag="OQQ48_002809"
     CDS             complement(11788..12354)
                     /gene="slp"
                     /locus_tag="OQQ48_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HCS1953515.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            complement(12709..13826)
                     /locus_tag="OQQ48_002810"
                     /pseudo
     CDS             complement(12709..13826)
                     /locus_tag="OQQ48_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         3950 a         3021 c         3154 g         4100 t
ORIGIN      
        1 attatgtgtc gtcgcggctt cgaaatggac tttgctgaac tgttgctgga agactacaaa
       61 gcctccctga aatatctcag cgatcacccg aaactgcagg gtattgccca gcagaacagc
      121 tttaaacaca cctgataaca taacgttgta aaaaccgaat gcccagcctt taaaaaaaca
      181 gctgggcatt cggttgctta ttaatcgcaa taatatattg gctgtctatt catcgtgttg
      241 ataagatata tgttatatga atgtttatta aatgtagcga ttgcctttaa tgtcacgaaa
      301 agggattatt tgcttgctat taatttcact gcgttcttaa acgcgttttt gtattataaa
      361 tccagtcatc ctgcccgtag aacatcacac atcatcatcc tgttctcccg cttcgtttaa
      421 atttatttat caatcaattt gacttaagag ggcggcgtgc tacattaatt aacagtaata
      481 tgtttatgta atattaagtc aactatgcaa tcattacatg ggaattgtct aattgcgtac
      541 gcaagacata aatatattct caccatggtt aatggtgaat atcgctattt taatggtggt
      601 gacctggttt ttgcggatgc aagccaaatt cgagtagata agtgtgttga aaattttgta
      661 ttagtatcaa gggatacgct ttcattattt ctgccgatgc tcaaggagga ggcattaaat
      721 cttcatgcac ataaaaaaat ttcttcatta ctcgttcatc actgtagcag agatattcct
      781 gtttttcagg aagttgcgca actatcgcag aataagaatc ttcgctatgc agaaatgcta
      841 cgtaaaagag cattaatctt tgcgttgtta tctgtttttc ttgaggatga gcactttata
      901 ccgctgcttt tgaacgtttt gcaaccgaac atgcgaacaa gagtttgtac ggttatcaat
      961 aataatatcg cccatgagtg gacactagcc cgaatcgcca gcgagctgtt gatgagtcca
     1021 agcctgttaa agaaaaaatt gcgcgaagaa gagacatcat attcacagtt gcttactgag
     1081 tgtagaatgc aacgtgcttt gcaacttatt gttatacatg gcttttcaat taagcgagtc
     1141 gcagtgtcct gtggatatca cagcgtgtcg tatttcattt acgtctttcg aaattattat
     1201 gggatgacgc ccacagagta tcaggagcga tcggcgcagg gattgccgaa ccgtgactcg
     1261 gcggcaagta ttgttgcgca aggaaatttt tacggcactg accgttctgc ggaaggaata
     1321 agattataga attttactca gacataaaaa aaacctggca taggggaccg ggaagaggat
     1381 agtctgccgt ctccagacta ataaaccgct ataacactcc ctgttggcac gggaaacttt
     1441 gtgctctcag taagttaaat ataactttta ctggaaataa gatcagccat ttttttataa
     1501 acataagcta tacgctgtgc gaaaatatat tcgtgctgca tttacttatt atcaattaac
     1561 tgttatgcaa aactactttg tggataaatt ttggtcctac caaatctggc agtttttgcg
     1621 ctaagaaaca gtctggcaac atttcattag tatactgaaa ttgaaataat cgcagtatga
     1681 aatataaggg ataatcatga ctcatgtctg ctcggtgatc ctcattcgtc gttcattcga
     1741 tatttatcat gaacagcata aaatatcgct gcataacgag agtatcgtgc tgctggagaa
     1801 aaatttggca gacgattttg cgttttgttc accggatacg cgacgactgg atatcgatga
     1861 gctgacagtt tgccattact tacaaaatat tcgtcagcta ccacgcaatt tagggttaca
     1921 tagcaaagac cgtctattaa ttaaccagtc accccccatg ccgctggtga cggcgatttt
     1981 tgatagcttc aatgaatccg gggtaaattc accgatactg agcaatatgc tctacctttc
     2041 ctgtttatcg atgttttctc ataagaaaga actgatcccc ttacttttca atagcatcag
     2101 cactgtttca ggaaaagttg aacgccttat tagctttgat atcgccaaac gttggtatct
     2161 gcgcgatatc gcggaaagaa tgtataccag cgagagtctc atcaaaaaaa agttgcagga
     2221 tgaaaatacc tgtttcagta aaatattact cgcttccagg atgtcgatgg ccagacgatt
     2281 actcgagtta cgtcaaattc ctctgcatac tattgcagaa aaatgtggct atagcagtac
     2341 gtcgtacttt ataaacacat ttcgacaata ttatggtgta acgccacatc agtttgcgca
     2401 acattcgcta ggtacctttt cctgacatat tttgcatttg aatattggtc aggatctcac
     2461 acctgcttca tgtgaaactc ttccctgatg atttctgccg ggctaccggc tagttctctt
     2521 tcgcagtttg ccaggcggta taggtatttt caggaggaaa agactcggca tgtttgggat
     2581 tattaagctg acaattcata cgattacggg aatgtgggtc agtattgtgc tgttcaaatt
     2641 gatgacgaat ggctggagtg gtttctactt tcagtgctgt gtactaagcc tggtattcct
     2701 gacggttagc tggttgttga gtggtgaatg gttagcagga aagagtaagg ctgaaccttc
     2761 acgttcaacc ttactctcat ttacacgtta cgctttttta aagcgggcaa agagatgttc
     2821 cactacaacg aaaaagaccg gaacgaagta aattgccagc actgttgcgg caaacatccc
     2881 gcccatcacg ccggtaccta ccgcgttttg cgcgccagaa ccggcaccat gactgataac
     2941 cagcggcagc acgccgagaa taaaggccag agaggtcatc aggattgggc gtaaacgcat
     3001 ccgcgccgct tcgatgattg cccctatcgg cgttttccct tctttctgca tcatctcaac
     3061 ggcaaattcg acaatcagga tggcgttctt ggcggaaagc ccgatggtgg tcagcaaacc
     3121 aacctggaag tagacgtcat tacttaagcc gcgcagatcg gtggccagta atgcgccaac
     3181 gacgcctaac ggaacaacca acatcaccga gaacggaatt gaccagctct catagagtgc
     3241 ggcgagggcg aggaacacca cgaccagtga aatggcatac agtgcaggag cctggtttga
     3301 ggataacgct tcctgatacg acagtccggt ccatgagtag ccgacgcctg ccggaagttt
     3361 agcgaccagg tctgccataa atttcatagc gtcaccggta cttttcccgg ccgccgcttc
     3421 acctaaaatc tccattgacg ggatgccgtt gtagcgttcc aatcgcggtg aaccataggt
     3481 ccattcggta gacgagtagg cagaaagcgg tgccatcgtg ccagaggcgt tgcgtacata
     3541 ccattggttg atgttatccg gcaacatacg gaacggcgtg cctgcctgga catacacttt
     3601 tttcacccgc ccctggttga ggaagtcgtt cacgtagctg ctgccgaagg cggtggaaat
     3661 tgtctggttg atatcagaca gcgccacacc cattgcctca gctttcgcgg cgttgacgtt
     3721 cactttgaac atcggcgtat cttccaggcc gttcgggcgt accccggtga cctggtcagg
     3781 cgattgcgct gccagtgata acagctcgtt tcgtgcctgg gttagttttt cgtgccccag
     3841 gttaccgttg tccagcagtt ccatatcaaa acctgacgcg gtacccagtt cagccaccgc
     3901 gggtaagttg aacgggaaga cgacggcttt attgatactg cttaacgcaa tcattgcccg
     3961 ctgaatgatc gcggtaaccg agttttcctc accgacacgt tcagaccacg gcttgagact
     4021 gataaacgcc aggccgttgt tttgcccctg gccgctgaag ccaaagccgc caacggtaaa
     4081 caccgactga acattatctt tctcttttgt aagataataa tccgtcacct gttgcagcac
     4141 tttcgtggtg ttaaccatgg tggcaccgga aggtaactgc gcggtggtca taaatacccc
     4201 ctgatcctct tctggtaaga aagaggtcgg tgtacgcagg aacagcaccg ccatcccggc
     4261 gcaaatcagc aggtagacca ccatgtagcg accggtacaa cgcaacagcg agcgggtgct
     4321 atcggtatag tgctgagttg atttttcaaa cagcgtgttg aagcgtgcga acagggcgtt
     4381 aggtttgtga ccgccttccg gcgcggcttt cagaatggtg gcgcacaggg caggggtcag
     4441 gctcattgcc acaaatactg aaagcagcat ggaggagatc agcgtgatgg agaactggcg
     4501 gtagatctcc ccggttgcgc cgctcataaa ggccatcggc ataaacactg cggaaagaac
     4561 aacagcaata ccgaccagcg cacgttggat ctgccccatc gatttatgcg tcgcttcctt
     4621 cggtggtagc ttatcttccg caatgacacg ctcgacgttc tccaccacca cgatggcgtc
     4681 atccaccagt aaccctatcg ccagcaccat cccgaacata gttaacgtgt tgatggtgaa
     4741 accgaccgcc gacaagatcg caaacgtccc gagaataacc accggtacgg caatcgtcgg
     4801 gatgattgtg gcacggaaat tctgcaaaaa cagatacatg accaggaaga cgaggatgat
     4861 agcctcaacc agtgttttaa aaacttcctg aatagaaatt tcgataaacg gcgtggtgtc
     4921 gtaaggataa accgtcttca gacttgcggg gaaataggct gataagcggt tcagctcctc
     4981 tttgactgcc cgcgaggtat ccagggcgtt tgctccggta gccagtttga tggcgatccc
     5041 ggcagcaggt ttgccgttat agcgcgccac ggtggaataa tcttccgctc caagttcgac
     5101 gcgggcgaca tcacgcagca gcacttgcga accatcttgc tgaactttca acaggatttt
     5161 gccaaattct tccggcgttt gcagacgcgt ctgcacaatg atcgaggcgt ttagttgctg
     5221 gtctgccgcc tgtggcatgc cacccagttg accaccggaa atctggttgt tttgcacctt
     5281 aatctgggaa ataacatcgg aaggtaccag attgtattta ttgagttttt gcgggtccag
     5341 ccagatacgc atggcgtact cggaaccaaa gagctgtacg ctaccaacgc ccgcggtacg
     5401 gcttagcggg tctttgatat tagacgctac atagtccgcg atatcgtact ggttgaggct
     5461 gccgttatca gaaataaacg ccgctaccat cagaatatta ctgctcgact tatcaacgct
     5521 aatcccctgc tgctgcaccg cttcaggtaa tgaaggcata gcgagttgca gtttattttg
     5581 cacctgaacc tgtgcgatat caggagatgt cccagtctcg aaggtcagag tgatagaggc
     5641 attacccgcc gcatcactgg ttgaagacat gtacatcagg ccatcaagcc cattcatatt
     5701 ttgctcaatc acctgagtga ccgagtcttc taccgtttgc gcatcggcac ctggataggt
     5761 agcgctgacg gtaatggtcg gtggcgcaat ctgcggatac tgcgcaaccg gtaagttcat
     5821 gatcgccaga ccacctgcaa gcatcataat aatggcaagt acccaggcaa aaaccgggcg
     5881 atcaataaaa tagttagcca tgaaagtccc cttaagcctg caacgttatt gtttcgattc
     5941 ggtgctggcg ttttcctggc tggaggaaat tgctcgtgct ttgatacccg gacgaatgcg
     6001 ttgcaaaccg gaaacgatca cccgatcgcc agcctgcaag ccagaggtga caacccactg
     6061 gtcgccgatg gctttgctgg cttcaatttc gcgtagctgc acaacatcgt ctttatccag
     6121 aatgagcgcc gttgctttac cctgggcgtt gtgggtgacg ccttcctgcg gcaccagtaa
     6181 tacattctgg cggctacctt catccactaa tgccgtgacg tacatgccag gcagcaagtc
     6241 accatttggg ttggggaaaa tcgcccgtaa cgtcacggag cccgtggttt catccaccgt
     6301 cgggtcggag aatttcagtg tgccggtctg gctgtagcgt ttaccatttt ccagattgag
     6361 ctgtactggc gtactgccct gaacctgttt gatttgccca ctggcgacct cttctttcat
     6421 gcgcaagaaa tcctgcaccg actgcgtgag atcgacataa atcgggtcca gacgttgtac
     6481 ggtaaccagc gaatctgcct gattagcggt aacgagtgcg ccgacggtca ccgacgattt
     6541 cccgctgacg cccgtaatcg gcgaggtgac attcgcgtat tgcagattga tcgtcgcctg
     6601 ttcaacagcc gctttggcga cggtgacatt ggcttctgct tcattcagct gggtgcgcgc
     6661 ggtgtcgtaa tcctgacggc taacgtagtt ggtcttcagc aacgatgcct ggcggttaaa
     6721 ggtgatgcgg gcattgctgg cggtagagag cgctttcgcc agcgagcctt tggcggagtt
     6781 tagctcggcc tgtaaaggtg caggatcaat ctgatacagc gaatcgccct ggttcacttt
     6841 atcgccttcg ataaagttgc gtttaatgat aataccgccc acctggggac gtatctcggc
     6901 aacttcataa ggaacggttc taccgggcaa ttcgctcaac acattgaccg aaccggggga
     6961 gagtgtgacg acaccgacct caggcgtcat ggcggcggcg ttttccgccg atttgtcatc
     7021 gcaggcggtg agcatcgcgc cgcagaataa caacggtatt aacagctttc ttcttctgtt
     7081 cattttagtc cctgaaaatt cttgagaaat aacggttctt taaattgcgc gcgaggagag
     7141 atatgaggga tttacagacc agcggcatag tggggttgtc agccagtaag gtgggataca
     7201 ggcacagtga tcgacatggt gaggtcaacg acaaaaaaaa tgttgccgtt ctgccaacag
     7261 ttcctgccag cattcgggca acgaagggtt ctactggtgg atacacatac caggggaata
     7321 aagtcgtttt agcctctcct ttagtaatca ccgggggtaa tgaaatcagc atctgcatcc
     7381 ctcgtcatgc cagccatcaa tttcagttgc ttatgtcctg actaaaaata agatgtgata
     7441 cccagggtga cgatgtcgct catacgtctt agtttcaact tgtacataat gttgaatttc
     7501 tgaatcttta atgtttgttg cgaaatattg agcgtcaacg tgatattgct ttcattttta
     7561 tttttcagaa tcagttgcat aattttgaac tgattatgtg aaaaacataa ttgtgaattc
     7621 ttatggggca agtgtttacc ataagtaatc tcaagaagat ttttgatact ttctttgcgg
     7681 ctaacaaaat aaataggcgc tttagctttt agtttactga tgtgataagg tgccaaaaca
     7741 ataattctct cggcatctaa tttctccaga aatttaatcg cttcttcatc aaggatatga
     7801 ttgtgatagg tatcaatgat aaccttgaag ggtttattgc ctactttttt gatctctgac
     7861 agggaattga tttttatcat ttcgtgatta tctttcaact gccaaaagcc ctgtaaaaga
     7921 aaagaatctt tcgtcatgag aaaaatcata acttgctcct tagccgttat cgtttcgaac
     7981 atgtcatcct tttcattatt tacatccttg tccgaatcgt tgttcaatat agtaaacgcc
     8041 tataagaact ttacccttcg tgaactaata cgtctattaa cactaatcaa tacttcagat
     8101 ttatccatca cgcactccat atattgatcc taagtaagat tcctggttgt tatcagcttg
     8161 taaatagtaa acaaaaagtt aacgtttgcg gattactgcc caagaataag ttatcaataa
     8221 ccagtttgtg atctctgaag aatattacta aagttaaaat gtaatccgat ttaaatatcg
     8281 agtctccttg tttcgactta agctggcaat tggattgcca gctttctttt aacgcattga
     8341 aattacctga gtaaataacg cattcatgtc ttcaggtttt tatatagcgt ctggagacac
     8401 ggatatttat gcaatgtaat caacgccagt tgcaaaaaaa tgaacccgga ttacttattt
     8461 aaccgcccat agcatcaacg cttacggaac tctttgttca ctttcccaca gcacttttaa
     8521 aacattaaac ctgactacgg aaaatatcag ccatgataat gtttgaaatg gctaatttgc
     8581 catggagtga aaaaaattag atgaaattca gtaggttgaa ataatcacta gcaggtaatt
     8641 atttcaatga tagtgcgcaa ttgatctaca acactgcgta gcggagagag tattaatcgg
     8701 atcatagtca catcaagtga ctatgatccg ggtgacaacc ggggtaatta ttgctgctta
     8761 acgaacaaac tggcgaagct gaacaggctg gcggcgctaa atatcagttc aattcccacc
     8821 agtgtggaaa ccagcgttac agacaccatc ggcgttgcac caaggaatat ccaggcaatg
     8881 acgatatcca gcacaccaat aacgagctgt agccagctgc ctttcattga acgctgacga
     8941 taccaactca tcaggcgaat aacccctgca acacagaaca aaccggcaat aaatgccgca
     9001 atggcaaaaa tgcccagctc cggtgcgcgg atgaagaaat agccgatcaa taaataggcg
     9061 actgcgacga ggaaaccgga taataccggc cagaaattat gactgcggtt gctgaataac
     9121 ccgacaataa gcgcaatacc cgagcagatt aataatgcac ccactactgt gcttaaaata
     9181 tcgccagaga cgaacgggaa actgatacac agcaacccga cgataaacag cagcacggca
     9241 ataaactgga ttgctctgcg atgtttttta agcatctcca gatcaaactt caaaattgtt
     9301 gccttatcta tatataacat agaaccaccc tataaaatta agaagaaaat cccctgctat
     9361 caatctatgc caaaaacgcg tctaagaatg cagtcgattt aataaaaatt tcctaattgc
     9421 agtatctgat gcatctgtaa ctcattgtat tgaaataaaa atatctgatt ttgatatttt
     9481 ccatcaacat gacatataca gaaaaccagg ttataacctc agtgtcgaaa ttgatttgtg
     9541 acggctcttt cactttatag ttgaggatat tacgatgaaa aaagtattag gcgttattct
     9601 tggtggtctg cttcttctgc cagttgtgag caatgcagcg gatgcgcaaa aagcagctga
     9661 taacaaaaaa ccggtcaact cctggacctg tgaagatttc ctggctgtgg acgaatcctt
     9721 ccagccaact gcagttggtt ttgctgaagc gctgaacaac aaagataaac cagaagatgc
     9781 ggttttagat gttcagggta ttgcaaccgt aaccccagct atcgttcagg cttgtactca
     9841 ggataaacaa gccaacttta aagataaagt taaaggcgaa tgggacaaaa ttaagaaaga
     9901 tatgtaattc cgggaatgcg ttacatcgta cttccttgca tattgaacag gccggaatat
     9961 cttctttaaa agcagctatt cctcctgttc atatataatc tatatattga atgggttaca
    10021 aaatgaatat ttcatctctc cgtaaagcgt ttatttttat gggcgctgta gcggctttgt
    10081 cactggtgaa cgcacaatct gcgctggcag ccaatgaatc cgctaaagat atgacctgcc
    10141 aggaatttat tgatctgaat ccaaaagcaa tgaccccggt tgcatggtgg atgctgcatg
    10201 aagaaacagt atataaaggt ggcgataccg ttactttaaa tgaaaccgat ctcactcaaa
    10261 ttcctaaagt gatcgaatac tgtaagaaaa acccgcagaa aaatttgtat accttcaaaa
    10321 atcaagcatc taatgacttg ccgaattaat gaggtacaag taaaaaggag tagcaagttg
    10381 agccatcttg ctgctccttt ttgcattttt atatgacagc agaatttatt atacgtctta
    10441 tactcgcggc aattgcctgt ggcgctattg gcatggaaag gcaaatgtga ggcaaaggag
    10501 cagggttacg cacacatgta ttaattggca tgggaagcgc cctgtttatg attgtttcga
    10561 aatatggttt tgctgacgtg ctgtctttag atcacgtcgg actcgacccc agccgtattg
    10621 ctgctcaggt ggtgacgggc gtcgggttta tcggtgcagg taacattctc gttcgtaacc
    10681 aaaatattgt aggtttgacg acggcagcgg atatctggat gaccgccgct ataggtatgg
    10741 ttattggcag tggtatgtac gaattgggta tgtatggttc agtgatgacc ttgctggtgc
    10801 tggaagtctt ccatcaatta accttccgcc tgatgaacaa aaattaccat ttacagctga
    10861 cattagtgaa cggtaatacg gtatcaatgc tcgactggtt taagcagcaa aaaataaaaa
    10921 cggatttagt ttcgttgcag gaaaatgaag atcatgaagt ggtcgccatt gatatccagc
    10981 ttcatgccac aacgtcgata gaagatctgt tgcgattatt aaaaggtatg gcaggggtga
    11041 aaggcgtttc aattagctaa tctgtgtggc agcgtagtca gactcaccgt aagcctgaaa
    11101 ttcacaccag ataatcaata tgctgatggc gtaccagctc attgatccgc ttaagattga
    11161 ggatcatcat gatattgtgt ttatggcagt agaacgtttt tagcgttatt ttcagtttct
    11221 ccagaatatc ctccgttgtc atttgattcg caatgcagct cagaacctga tgttgcttgc
    11281 gcgaaagtga cacagccgct tcaggcacag agcagtaact gccattgatg agtgcgacaa
    11341 aatcgatgat agtgatagtc cgcggaacaa aggtcaccgg cactttcccc aggcagcgtt
    11401 taatattaaa gggggagaaa ataatcacat ggacaggttt taatcgttca atttgagtga
    11461 gaaaattaga atgaaataca ttattcatta atgtatcaat aatgacaaag gcattctgat
    11521 gcaacattcc gtcgatctct tcttcgttct gtatatgaac gacattacct ttgctcaaaa
    11581 tgtttttcat cgcggtgaag aacatcgtat ccctggtaat tataagaaac ataaccgact
    11641 cctggtgcgg acattaattt tataatcagt ttgccaaagg taattaacag gacagggctt
    11701 ataataataa tagttagctt gctgtgtatt aattttatgt aatgcacgat gcagatgctg
    11761 agaaagcatc tgcatctttc ggtggtgtta tttgaccagc tcaggtgtta cctgactcac
    11821 cgcattggtg tagtaaggcg caccccagcc cggttccggc cagaatgcac catagccgta
    11881 atcccatagg ttataggtgg tattaaccac ttctctcaaa tgccacacct ggatgccctg
    11941 catattcact tccaggaagt tatacgggac tttattgata aagccaggtt gttcaccctg
    12001 aatggtaccg aggatggtaa caaagtgatt acgatagttc actgggtcaa ggaagccgct
    12061 ttgtctggcg agcagtcggc cctgatagtt ggcttcaata tcaggtttcg catagctatc
    12121 caacggtaat acagcgattt ctaacaacgt atccgttttg ccattgataa cgttgataac
    12181 cttcccacca aagcgcgctt gttgaccaac atataacccc ggctggttat gaacagcaac
    12241 aaaacttttt tgaatatcag gttggttatt gcctttgata ttttgcggaa ttgaactaca
    12301 tgcggcaagc aaaaatgaaa ggctgaggat gagtgcacct tttgtcatgt tcatgttact
    12361 atccttatca acaaatatta aaaccataat agatatttct ttatagtttt catctgattc
    12421 tgaggcgata tgcctgatag taacgtaaac ctgtctgatg tttatatccc ttttgtttag
    12481 ttcaattcca ttgattttgg ggcgtggcat aagtatccat cgccccataa tataatcctt
    12541 tgttttaaaa ttacataaaa tctattttta ttcaataatt ataaagtgct aataatcaat
    12601 attatggtat tatattctga taatgatttg ataagcgaat tcttttgcca gatggtattt
    12661 ctatttccac cccataattt atatcttttt cattaggaag tggcctcttt aaataaggcg
    12721 tgtgtgacat tttttttggg ttaagcgtct cgaattgaaa tacttcgaac tgatattcag
    12781 acatttctgc ccatgtttga attttattgt atcgagagag tgtcgtagag gctttaactt
    12841 gcttataaca ttgtttttgg aattcttgct gatattttaa tatgtcattg tatgaaagat
    12901 tggttatcct ggcctctaaa aatttaatta ttgtcgaagc aatttccgct tttgctcgta
    12961 aaaagttgtc attaatggtt aataatgttg aaaataaacc ttcgatttgt ttgtcaagat
    13021 attgtccatc tttatataat ttgggggggt agggtgttct ttatgtaaaa aatagtgtca
    13081 cctaaatttg cgtcagtgaa atttatatta agtagatctt cgaaataaaa ccttgacaag
    13141 gttagaacga acccggaaag gttctgtcct gtcatattga aatcaataaa gttggttccg
    13201 gcaaagactt tcatcctgtt tttattttca ctgtgctcaa tatataactt cgtcattttt
    13261 tgatacaggc tttgaatatc ttttatatta gttggtaagt cagctcgttg ttcagcaata
    13321 aaattagtat ctgaaaacat ggtttggaca ctgaatgtta tgggtattcc ctcttctacg
    13381 ctaattttag ttatgattgc cttgtcgggt ttaggattag cgacaacaat gattcgacat
    13441 tcagcacttt tgtttttttc atatctaatg ttattaatct gattaagtat tgattgtgtc
    13501 tgttgcgtaa gtggtgtcga acttatcgtt tcaatgttat taataagact tacaagttga
    13561 accacaactt ttgagtagtc tgcatttgta tatgtggtgt tcgtgtggca tgcattaatt
    13621 tgttggagtt gttgttgcgc taaatcaaat tgttcagggt tgtatccttc tccagagaat
    13681 gtggaattaa tgggttgttg ttcttcccaa ttatcacaag gccacttcgg tgtgtctggc
    13741 gttttaacat tatcggtatt tgttgtgcag ttataaatat tgccgtgata tgtatttatt
    13801 atacctgggg taaagtcaat tgacataaca ttccttattc atcatctgtt ttatgctaag
    13861 gtttttaaat tggataatta gaacaggaat ggattttctt ctacacacaa taaatataat
    13921 gcgttatatc ttcctgatat aattaccttt aaattccctt tcatttccac ccggtaatat
    13981 ttgtgtataa aaaaagcagt gatagtatcg taaaggaaaa cgttaacaac tatcaaaaag
    14041 cgctcatttt aaaaagttat tatgttctcc gatagtgtgt gaagtaatat ttttgtgatg
    14101 ttttagatgt gtaataatga accaggttaa agtgtattta aaaatgtgtt gtattagcga
    14161 ctgaaatctg ccttttaaaa aggaggaggg tactgagaca aagagtcatt gcttctccga
    14221 taaaa
//
LOCUS       DAJVUJ010000104        14200 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.104, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000104 DAJVUJ010000000
VERSION     DAJVUJ010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14200
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.104"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(92..388)
                     /locus_tag="OQQ48_002811"
     CDS             complement(92..388)
                     /locus_tag="OQQ48_002811"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1953516.1"
                     /translation="MRFLLANTFCKIVKQFSFIDRIRIYSLHPCLNKHLSIQSNRDWL
                     SMSIFSDYSFSIEMHNNLTIDYYLALSSTKGSGITNIISIILQQAQDYDVAKIT"
     gene            672..2303
                     /gene="oppA"
                     /locus_tag="OQQ48_002812"
     CDS             672..2303
                     /gene="oppA"
                     /locus_tag="OQQ48_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HCS1953517.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            2389..3309
                     /gene="oppB"
                     /locus_tag="OQQ48_002813"
     CDS             2389..3309
                     /gene="oppB"
                     /locus_tag="OQQ48_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HCS1953518.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            3324..4232
                     /gene="oppC"
                     /locus_tag="OQQ48_002814"
     CDS             3324..4232
                     /gene="oppC"
                     /locus_tag="OQQ48_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HCS1953519.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFIHNRAAVASLM
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            4244..5257
                     /gene="oppD"
                     /locus_tag="OQQ48_002815"
     CDS             4244..5257
                     /gene="oppD"
                     /locus_tag="OQQ48_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HCS1953520.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPEHELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTLGRLCACFKPVE
                     ELL"
     gene            5254..6258
                     /gene="oppF"
                     /locus_tag="OQQ48_002816"
     CDS             5254..6258
                     /gene="oppF"
                     /locus_tag="OQQ48_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HCS1953521.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGIKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(6311..6640)
                     /locus_tag="OQQ48_002817"
     CDS             complement(6311..6640)
                     /locus_tag="OQQ48_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HCS1953522.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPVEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(6675..8135)
                     /gene="cls"
                     /locus_tag="OQQ48_002818"
     CDS             complement(6675..8135)
                     /gene="cls"
                     /locus_tag="OQQ48_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCS1953523.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEIVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            8278..8451
                     /locus_tag="OQQ48_002819"
     CDS             8278..8451
                     /locus_tag="OQQ48_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HCS1953524.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(8506..9759)
                     /gene="kch"
                     /locus_tag="OQQ48_002820"
     CDS             complement(8506..9759)
                     /gene="kch"
                     /locus_tag="OQQ48_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HCS1953525.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCLAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(10059..10355)
                     /locus_tag="OQQ48_002821"
     CDS             complement(10059..10355)
                     /locus_tag="OQQ48_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HCS1953526.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            10570..11298
                     /gene="tonB"
                     /locus_tag="OQQ48_002822"
     CDS             10570..11298
                     /gene="tonB"
                     /locus_tag="OQQ48_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HCS1953527.1"
                     /translation="MTSMTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAP
                     AQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPK
                     PKPVKKVQEQPKRDVKPVESRPASPFENTALARLTSSTATAATSKPVTSVASGPRALS
                     RNQPQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRY
                     EPGKPGSGIVVNILFKINGTTEIQ"
     gene            complement(11338..11736)
                     /gene="yciA"
                     /locus_tag="OQQ48_002823"
     CDS             complement(11338..11736)
                     /gene="yciA"
                     /locus_tag="OQQ48_002823"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HCS1953528.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(11841..12380)
                     /locus_tag="OQQ48_002824"
     CDS             complement(11841..12380)
                     /locus_tag="OQQ48_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HCS1953529.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(12410..13153)
                     /locus_tag="OQQ48_002825"
     CDS             complement(12410..13153)
                     /locus_tag="OQQ48_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HCS1953530.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAVLLIYLFRLYMLIRQ"
     gene            complement(13181..13270)
                     /locus_tag="OQQ48_002826"
                     /pseudo
     CDS             complement(13181..13270)
                     /locus_tag="OQQ48_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            13510..14148
                     /gene="ompW"
                     /locus_tag="OQQ48_002827"
     CDS             13510..14148
                     /gene="ompW"
                     /locus_tag="OQQ48_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HCS1953531.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
BASE COUNT         3827 a         3318 c         3474 g         3581 t
ORIGIN      
        1 cgcagttatc aaaagcaaaa ggaataggta aaaatattct tctcaaatta cagttagtta
       61 taaggatttt cttaactgct tctcctcacc atcatgttat tttcgccaca tcataatcct
      121 gggcttgctg aagaataatt gaaatgatat tagtaattcc actgcctttg gtagaggaaa
      181 gtgctaaata ataatcaatt gttaaattat tgtgcatttc aatactgaaa ctgtaatcag
      241 aaaagataga catgcttagc caatctctat ttgattgaat tgaaagatgt ttgttaaggc
      301 atggatgcaa gctatagatt ctgatacggt caataaaaga gaattgctta acaattttgc
      361 aaaatgtatt ggcgagtaag aaccgcattt agtactttcc gggcaaccgc cagacgattc
      421 tttattggta atgagaataa ttaacaatta aagagcgtcg cgaaagaata atgtgtctcg
      481 acaggggaga cacagtacga atcgacataa ggtgatcgtc tgaatcacca gaataaataa
      541 agtcggtgat agcaatccgt aacgataaag taacctgaca gcagaaagtc tccgagcctg
      601 tgcagggtcc caatccggga ttacacatgc tggttaatac cagtaattat aatgagggag
      661 tccaaaaaac aatgaccaac atcaccaaga gaagtttagt agcagctggc gttctggctg
      721 cgctaatggc agggaatgtc gcgctggcag ctgatgtacc cgcaggcgtc acactggcgg
      781 aaaaacaaac gctggtacgt aacaatggtt cagaagttca gtcattagat ccgcacaaaa
      841 ttgaaggtgt tccggagtct aatatcagcc gagacctgtt tgaaggctta ctggtcagcg
      901 atcttgacgg tcatccggca cctggcgtcg ctgaatcctg ggataataaa gacgcgaaag
      961 tctggacctt ccatttgcgt aaagatgcga aatggtctga tggtacgcca gtcacagcac
     1021 aagactttgt gtatagctgg caacgttctg ttgatccgaa cactgcttct ccgtatgcca
     1081 gttatctgca atatgggcat atcgccggta ttgatgaaat tcttgaaggg aaaaaaccga
     1141 ttaccgatct cggtgtgaaa gctattgatg atcacacatt agaagtcacc ttaagtgaac
     1201 ccgttccgta cttctataaa ttacttgttc acccatcaac ttcaccggtg ccaaaagccg
     1261 ctatcgagaa attcggcgaa aaatggaccc agcctggtaa tatcgtcacc aacggtgcct
     1321 ataccttaaa agattgggtc gtaaacgaac gaatcgttct tgaacgcagc ccgacctact
     1381 ggaacaacgc gaaaaccgtt attaaccagg taacctattt gcctattgct tctgaagtta
     1441 ccgatgtcaa ccgctaccgt agtggtgaaa tcgacatgac ttataacaac atgccgatcg
     1501 aattgttcca gaagctgaaa aaagagatcc cggacgaagt tcacgttgat ccatacctgt
     1561 gcacttacta ttacgaaatt aacaaccaga aaccgccatt caacgatgtg cgtgtgcgta
     1621 ccgcactgaa actaggtatg gaccgcgata tcattgttaa taaagtgaaa gcgcagggcg
     1681 acatgcccgc ctatggttac actccaccgt atactgatgg cgcaaaattg actcagccgg
     1741 aatggtttgg ctggagccag gaaaaacgta acgaagaagc gaaaaaactg ctggctgaag
     1801 ctggttatac cgcagacaaa ccgttgacaa tcaacctgtt gtataacacc tccgatctgc
     1861 ataaaaagct ggcgattgct gcctcttcat tgtggaagaa aaacattggt gtaaacgtca
     1921 aactggttaa ccaggagtgg aaaacgttcc tcgacacccg tcaccagggt acttttgatg
     1981 tggcccgtgc aggctggtgt gctgactaca acgaaccaac ttccttcctg aacaccatgc
     2041 tttcgaacag ctcgatgaat accgcgcatt ataagagccc ggcctttgac agcattatgg
     2101 cggaaacgct gaaagtgact gacgaggcgc agcgcacagc tctgtacact aaagcagaac
     2161 aacagctgga taaggattcg gccattgttc ctgtttatta ctacgtgaat gcgcgtctgg
     2221 tgaaaccgtg ggttggtggc tataccggca aagatccgct ggataatacc tatacccgga
     2281 atatgtacat tgtgaagcac taatggcaat acgtggggca ggagtgtcct gctccacggt
     2341 gtctgatttt tatcgcatta cagaaggcac aggccagaag gtagggcaat gttaaaattt
     2401 attctacgtc gctgtctgga agcgattccg acgctattta ttcttattac tatttcgttc
     2461 tttatgatgc gcctcgcgcc gggaagccct tttaccggcg aacgtacttt accgccagaa
     2521 gtgatggcca atatcgaagc gaaatatcat cttaatgatc cgatcatgac acagtatttc
     2581 agctacctga aacaactggc gcacggtgat ttcggtccat cgtttaaata taaagattat
     2641 tcggtcaatg acttggttgc atccagtttt cccgtttctg ccaaactggg agccgcagca
     2701 tttttccttg cggtaatact gggtgttagt gctggcgtta ttgccgcatt aaaacaaaac
     2761 accaaatggg actataccgt gatggggctg gcaatgaccg gggttgttat ccccagtttt
     2821 gtggttgcac cattattagt catgatattt gcgatcattt tgcattggct gccgggcggt
     2881 ggctggaatg gtggggcgct taaattcatg atattgccga tggtggcgtt gtcactcgct
     2941 tatatcgcca gtattgcgcg tattacccgt ggctctatga ttgaagtatt acactccaat
     3001 tttattcgta ctgcccgggc gaaagggtta cctatgcggc ggatcatttt acgtcacgca
     3061 ttaaaacctg ctctgttacc cgtgctctct tatatgggcc ccgcatttgt cggcattatt
     3121 accggttcta tggttatcga aaccatttat ggtttgccgg ggattggaca actgttcgtt
     3181 aatggcgcat tgaaccgtga ctattcctta gtgttaagcc tgaccatcct ggttggtgct
     3241 ttaaccattt tgtttaatgc cattgtcgat gtgctatatg cggttatcga cccgaaaatc
     3301 cgttactgat actggagctc gcgatgatgt taagtaagaa aaacagcgag acgctggaaa
     3361 atttcagtga aaagctggag gtcgaagggc gcagcttgtg gcaggacgca cgtcgacgtt
     3421 ttattcataa ccgtgcggcg gttgccagtc tgatggtgct ggtgctgatc gcgttatttg
     3481 taatcctggc accgatgctt tcccagtttg cctatgacga tactgactgg gcgatgatgt
     3541 ccagcgcccc ggatatggag tccggtcact actttggtac tgactcatcc ggtcgcgacc
     3601 tgcttgtgcg cgttgcgatt ggtgggcgta tctcactcat ggtcggtgtt gctgcggcac
     3661 tggtggctgt ggtcgtcggg acactttatg gttcgctttc cggttatctg ggcggtaaag
     3721 tggattcggt aatgatgcgt ctgctggaaa tcctcaactc cttcccattc atgttcttcg
     3781 tcattttgct ggtgaccttt ttcggtcaaa acatcctgct gattttcgtg gcgataggca
     3841 tggtttcctg gctggatatg gctcgtattg tgcgtgggca aaccctgagt ctgaagcgca
     3901 aagagtttat tgaggcggca caagttggcg gtgtatcgac atcgggcatt gttattcgcc
     3961 acattgtgcc gaatgtactc ggtgtggtgg tggtctacgc atcacttctg gtgcccagca
     4021 tgatcctctt tgaatctttc cttagcttcc tggggttggg tacgcaagag ccgttaagca
     4081 gctggggtgc attgctgagt gatggcgcga actcgatgga agtctctcca tggttattgt
     4141 tgttcccagc gggattcctc gtggtgacgc tgttttgttt caactttatc ggcgatggct
     4201 tgcgtgatgc cctcgacccg aaagatcgtt aaggagtgca gccatgagcg taattgaaac
     4261 tgcaactgtg ccgctcgcac aacaacaggc tgacgcactg ctgaacgtga aagatttgcg
     4321 tgtcaccttt agtaccccgg acggcgacgt cacggcggtc aatgatttga atttttccct
     4381 acgtgccgga gaaacgctgg gtattgtagg tgagtctggt tcgggtaaat cgcaaactgc
     4441 atttgcgttg atgggcctgt tggctgccaa cggacgtatt ggcggatcgg caaccttcaa
     4501 tgggcgtgaa atcctcaatt tgccagagca tgaactcaat aaactgcgcg ctgaacaaat
     4561 ctcaatgatt tttcaggacc caatgacttc gttgaatccc tatatgcgcg tcggtgagca
     4621 gttgatggaa gtgctgatgc tgcataagaa catgagcaaa gctgaggcgt ttgaagagtc
     4681 ggtgcggatg ctcgatgcgg taaaaatgcc ggaagcacgt aaacgcatga aaatgtaccc
     4741 gcacgagttt tctggcggca tgcgtcagcg agttatgatt gcgatggcat tgctatgtcg
     4801 acctaagctg ctgattgcgg atgaacccac tacagcgctg gacgtcaccg tacaggcgca
     4861 gatcatgacg ctattaaatg aactgaaacg ggaatttaat accgccatca ttatgattac
     4921 ccacgatctt ggcgtggtgg cggggatctg tgacaaagtg ctggtaatgt acgccgggcg
     4981 cacgatggaa tatggcaacg cgcgcgatgt cttttatcaa cccgttcatc cttattctat
     5041 cggtttgctc aacgcggtgc cgcgtctcga tgcggaaggt gaaacaatgt tgaccatccc
     5101 gggtaatccg ccaaacctgc tgcgattacc gaaaggttgc ccgttccagc cacgttgtcc
     5161 gcatgcgatg gaaatttgta gtagcgcacc gccgctggaa gagtttacgc ttggccgtct
     5221 gtgtgcttgc tttaaaccgg tggaggaact gttatgaatg ctgtaaccga aggaagaaaa
     5281 gtcctccttg aaatcgccga tctgaaagtg cactttgaaa tcaaagatgg caaacagtgg
     5341 ttctggcaac cgccgaaaac gctcaaagcc gtcgatggtg tcacgcttcg cctgtatgaa
     5401 ggggaaacat taggtgtggt aggggaatcg ggatgcggta agtccacctt tgctcgcgcc
     5461 atcattggcc tggttaaagc gaccgacggt catgttgcct ggttaggtaa agagttgctg
     5521 ggcattaagc ccgatgaatg gcgtgccgtt cgcagtgata ttcagatgat tttccaggat
     5581 ccgttggcat cgctaaaccc gcgcatgacc atcggcgaga tcatcgctga accactgcgt
     5641 acttatcatc cgaaaatgtc acgtcaggaa gttcgcgagc gcgtgaaggc gatgatgctg
     5701 aaagtcgggt tattgcctaa cctgattaac cgctatccgc atgagttctc cggtgggcag
     5761 tgccagcgta tcgggattgc tcgtgctctt attcttgaac cgaagctgat tatctgtgat
     5821 gagccggtgt cggcgctgga cgtgtcaatt caggcgcagg tggtcaacct gctccagcag
     5881 ctgcaacgtg agatgggatt gtcattaatt tttattgctc atgacctggc cgtggtaaaa
     5941 cacatttccg atcgtgtgtt ggtgatgtat ctcggccatg cggtagaact ggggacctat
     6001 gatgaggtct accacaatcc actacatcct tacaccaggg cattgatgtc ggcagtcccc
     6061 atacctgatc cggatctgga gaagaacaaa accatccagt tactggaagg ggaattaccg
     6121 tcgccgatca acccgccttc cggttgtgtt ttccgtaccc gttgcccgat tgccggtccg
     6181 gagtgcgcca aaacacgtcc tgtgctggaa gggagtttca gacacgccgt ttcttgcctg
     6241 aaagtcgatc cgctttaaac acaataaggg ctgacagttg tcagcccttt ttcacgccga
     6301 aagcgattat ttattcccgc cagatgatat ggcaaagttt gtgatctttt tcgcggcata
     6361 acagaatgcg ggcgaaaaca tcgttgattt caccatcctc actgtccgcc agaccaatca
     6421 ccacttcggc aaaaaagtcc gggttcaaat cgaaatcaac atgttcctgc cagtcttcca
     6481 ccggatcgaa taactccgcg ccgccgcgct cttcaaactg aagattgaac agcagaacat
     6541 cagctgggtc gagattgtcg gcagccagtt cgagaaaaat atcgtaggcc tgctcaagcg
     6601 tttcatcttc agtcaggcga ttgtttagat ccatatccat agtcactacc tgtttaacct
     6661 ctgttggaga cgttttacag caacgggctg aagaagtaaa acagtcgctc ggcgacacgt
     6721 tgccataatg gacgttttag ccataaacgg gcatcgagca gacgtgaacg cgaaatataa
     6781 tcgtcctgaa cggcggcaag gtctgcacca aaacctttat cgtcgattgc cagggtaatc
     6841 tcgaaattca gccacagact acgcatatca aggttaacgg tgccaaccag acttagttcg
     6901 ccatcgacca gcacgctctt ggtatgcagt aacccgcctt caaactgata aattttaacc
     6961 ccagcagcaa gaagttccgt aaagaatgcg cgactggccc agccgaccag catcgagtca
     7021 ttttttcgcg gaaggataat actgacatcc accccgcgct gtgccgccgt gcaaatcgca
     7081 tgaagtaaat catcgcttgg cacaaagtag ggcgtggtca tgatcaaata ttcacgcgcc
     7141 gaataagccg cagtcaataa tgcctggtga atgagatctt ccggaaagcc ggggccagaa
     7201 gcaattgtgt gaatggtgtg accgctggcc tgttcaaacg gcataatatt gacatctggt
     7261 ggtggcggca gaatacgttt tccggtttca atctcccagt cgcaggaata aataatcccc
     7321 atcgcggtgg cgatggggcc ttccatacgc gccatcagat caatccattg ccctacgccc
     7381 gcatcttgtt tgaagtagcg aggatcgacc atattcatgc tgccggtgta cgcgatgtaa
     7441 ttatcgatca tgatcatctt gcgatgttgg cgcaggtcca tacggcgtaa aaacacacgc
     7501 atcagattga cctttaaggc ttcgaccact tcaataccgg cattacgcat tagctcgggc
     7561 cacgggctgc ggaaaaaagc cacactcccg gcggagtcga gcatcaatcg gcaatgaatg
     7621 ccgcgtcgcg cagcggccat taatgattca gccacctggt ccgccatgcc gccgggctgc
     7681 cagatataaa acacaatctc aatattatgg cgcgcgagct ggatgtcgcg gattaacgcc
     7741 tgcatcacat catctgactc ggtcatcagt tgtagctgat tccctttgac cccagcgatc
     7801 ccctgacgac gctcgcaaag cttgaataat ggcgcagcga cactgctatt ttcttcggcg
     7861 aagatatgct tacaggcttt aaggtcgtta agccattttg cggtggaagg ccacatcgct
     7921 ctggcgcgct cagcgcggcg tttgcctaaa tggagctcgc caacggcaag ataggcaata
     7981 attccgacta acggcagaat gtaaataatc aacagccagg ccatcgcgga gggaactgcg
     8041 cgtcgtttca ttagaatgcg taaagttacg cctgcaatga gcaaccagta tcccagaatg
     8101 gccaaccaac tcaccaacgt ataaacggtt gtcatagatt agaaatcctt ttgaaagcgc
     8161 attgttatga gtttacgcat atggattcat ctggcaaata aaaacgcggg aaaagcactg
     8221 gtcagcgtcg ggggcggggg tataatgacc attctgttat tgcatagagt agttaacatg
     8281 aagcggagta gaacggaagt ggggcgctgg cgcatgcagc gtcaggctag ccgacgtaaa
     8341 tcgcgttggc ttgaggggca atcgcgccga aatatgcgta tccacagcat caggaagtgc
     8401 attctaaaca aacagcgtaa ctcgttattg tttgcgatct acaatatcta aatgtactgg
     8461 gcaccattgc ggtgcccaat aagtttactc ctccatcttt aaactctatt tttgcgccga
     8521 ttctttactg tcagctttcg tttcttgttc atcgttatcg ctgaaaatgc catgaccgga
     8581 gttcaacaac attgaaacga gcatatcatt attaatctct tcaccattta aaactcgcgc
     8641 cagaatttcg ctgccaaaca gttgcggtga gagaatgata tccggatgta ccatcttaat
     8701 cttattcagg tttttgctat cactgacggc gagaactgtt ttgacatcac tgctcatatc
     8761 ttttgccgag agtacaacaa acgcgttatc tgcatcgtta tcactcagcg ccagaatggc
     8821 caggcaacga tcgattcccg ctttctttaa tactgaactg tcattactgt caccggggat
     8881 aacatcagcg ttatcgccta agcgttgctc aagttgcttg atatcatctt caggcaagtt
     8941 gctgataacc gttacgtttt gtccgcgttg attcagttgc agaatcgtat tgatggcgag
     9001 aatcgaatgt ccgcaaacaa taaaatgatc tttacgatgc attgtatgat tgtttccttt
     9061 tacaagtttg ttgaatcccc cgcggataag cggaccaaaa attgaggtca tagatgtggc
     9121 aaaaacggta ataccggaaa taatgaccga aatagtgaac aatcgtgctg attcagaaac
     9181 agggacaata tcgccatagc cgacggttga catggtttct atcgaaaaat agaacgcggt
     9241 catcaaactt tctattcgcg gattaaaacc ttcgcttaaa taaagcgcac cgtaggttga
     9301 gtaaaacagt aatgtcgtga aactaataaa tgcaaaaatt gtcccggctg cggcgctact
     9361 gtgggagaag tctttgcgca gtatcagcaa aaacaccagc gtaaaaatgc aaaatccaat
     9421 actaaatttc aaccagggat aaaaatgcag ggtataaatt agcgctatca acaacaaaat
     9481 gatactgatt gcccatgcga gctttgcccg gaacagtaag ccaatggagt tcaggacaag
     9541 aaagacgccg agcataaata gtggggcgtt tgccagcaag gagagatcca actctgaaaa
     9601 tgctttgata tggaaaatat ctagcaaatt gaccgacata ccatagattg ttttaaaaat
     9661 aagcaatcca tttaaaaaga cggccagcgc gagaatgtcg tgccgtaggg tcacccataa
     9721 atttgttgct gtttgtttga atgtagccca gtgactcact gatacctctg taaccacaat
     9781 tttgtttaat tatcttagcg tgcacatttt ctgaacatac atgcagcgcg tgaatgtgtt
     9841 aaacaatagt agatgacttt atccgcagta atcaaaggtt ttgagcgagc aactgtacct
     9901 caggaaaggt catttttcct gaatatgctc acatcatata aagaaataca gataaagtta
     9961 ttatctgctt gtggtggtga atgcactgac cggctataag gaaaggccaa acaagaacac
    10021 ggttgcaaaa accgtgccct taaatattga atctctattc agaacacttt cttaaatggt
    10081 ttcactgaaa cgtgttcata gactcctgcc gctacgtacg ggtcagcatc ggcccaggcc
    10141 tgagctgctt ccagcgattc aaattcagca ataacggttg agccagtaaa tcccgcagcc
    10201 cctggatcgt tactgtctac cgctggcatt ggaccagctg tcaacaaacg accttcatca
    10261 tgcagtaact gtaaacgtgc taaatgtgcc ggacgaacgg aaaggcgttt ttcgaggcta
    10321 tcagctttgt cttgagcgta aataacatac aacacgggca caactccttg ttcgggaaag
    10381 ttgtaaagta cgttatttga aagggcgaag atctgcaacg gaaagatgat gtctttgtta
    10441 agaccatgca taaagtaagg ttaattacgg caaaaatgac attttcactg atcctgatcg
    10501 tcttgcctta ttgaatatga ttgctatttg catttaaaat cgaggcttgg tttttctact
    10561 gaaatgatta tgacttcaat gacccttgat ttacctcgcc gcttcccctg gccgacgtta
    10621 ctttcggtct gcattcatgg tgctgttgtg gcgggtctgc tctatacctc ggtacatcag
    10681 gttattgaac tacctgcgcc tgcgcagcca atttctgtca cgatggttgc gcctgctgat
    10741 ctcgaaccgc cacaagccgt tcagccgcca ccggagccgg tggtagagcc agaaccggaa
    10801 cctgagccga tccccgaacc gccaaaagaa gcaccggtgg tcattgaaaa gccgaagccg
    10861 aaacctaagc caaaaccgaa gccggtgaaa aaggtacagg agcagccaaa acgcgatgtc
    10921 aaacccgtag agtcgcgtcc ggcatcaccg tttgaaaata cggcactggc acgcctgaca
    10981 tcaagtacag caacggctgc aaccagcaag ccggttacca gtgtggcttc aggaccacgc
    11041 gcattaagcc gtaatcagcc gcagtatccg gcacgagcac aggcattgcg cattgaaggg
    11101 caggttaaag ttaaatttga cgtcacgccg gatggtcgcg tggataacgt acaaatcctc
    11161 tcagccaagc ctgcgaacat gtttgagcgt gaggtgaaaa atgcgatgcg cagatggcgt
    11221 tatgagccgg gtaagccagg cagtgggatt gtggtgaata tcctgtttaa aattaacggc
    11281 accaccgaaa ttcagtaagc agaaagtcaa aagcctccga ccggaggctt ttgactatta
    11341 ctcaacaggt aaggcgcgag gttttccttc aggatcaacc gcgacatact taaataatgc
    11401 ttctgtcgct ttatagcgtt gcccgattgg ttcagacgct acttttttca cccacacttc
    11461 aatattaatg ctgaccgatg tcgtcccttt ctggacacag cgtgcatagc agcacactac
    11521 atcgccgacc gcaaccggcc gtaagaaagt cattccttca acccgcacgg tcactacgcg
    11581 accgtgggca atttctttcg ccagaatagc gccgccaata tccatttgtg acattaacca
    11641 accaccaaag atgtcaccat tggcattggt atcggcgggc atggctaaag tacgtaaaac
    11701 aagatcgccc tgagggacgt tatgtgttgt agacatggta aaaccgactt aaaaggaaaa
    11761 tcacaggcgc gatgctacta tgattttcct gaggagaaca gaggagatac agcgccagcc
    11821 ccgaaggact ggcagtctgg ttaggattta tcttcctgcg gcatgtggcg gtagatatag
    11881 ataccgctta acaatgtgaa gattaaggta agggcggtca gtccaaagac tttaaagttg
    11941 acccaaatat tttgcggcag ccagaatgcg atgtagatgt ttgccagacc gcaaaggata
    12001 aagaaaacag cccaggccag attcagcttc gaccataccg gttgcggcag cgtgagttct
    12061 ttacccagca tccgctgaat tagcggcttt ttcatcaccc attggctgac taacagggct
    12121 cccgcaaaca gagcataaat gactgtaacc ttccatttaa taaactcatc attgtggaag
    12181 aacagcgtca agccaccgaa gacgaccacc agaacaaaag tgatcagggc catcttctca
    12241 accttacgaa agcgaaccca gctatagata agcacaatcg ccgtggcgac gatcagcgcc
    12301 gcagtagccg catagatgtc ataaatcttg taaaacgcga aaaagacaac cagcggtaaa
    12361 aaatcaagaa actgcttcat tttacgattc cgtaatcaaa tgcttaaggt tattggcgaa
    12421 tcaacatata cagacggaac agatagatga gcaatacggc tgaaatcagg ttgctcaagg
    12481 tgttcgccag tacggcacca atttccgggg ttaatgcggc aaaagaagag gcaaaaagca
    12541 gcaacagtgt ttttgccagc aaccagctca gtactgcggg tgccaccaga cgcatattcg
    12601 cccaagtcag ccgcatactg ctacgcatcg aggcaaaaat gcccattttg tcctgtacca
    12661 gcatcaccgg tgccagggcc agtaaaatgg ccatgataat tcccggaacg accactagca
    12721 tgatgccaat ctgtactaaa agggtagtca gaaaaatcag aataaataac tttggcaata
    12781 tcggtgcact ggcaccaata gctcgcagcg cactgactct ctgacccgca gacaccagct
    12841 ggataattaa tattacgcct ccggcgagaa tggcgttacc gattaatcct gaaaaagtgg
    12901 acgccgctga agcctgcagc aaaatttgtt gctgttccgg tgacatattc tgaaccaggt
    12961 cgaacaaccc actactgccg ctaacgggca cgccgtcatt gagctgcgca agctgtgcat
    13021 cactgggtga gaaaacatgc cctaacacca ctgtgataaa cgcacatagc aacgatacca
    13081 acagaatggt cataaattga ttacggaaga aatttccggt gtcacggtat acggactgcg
    13141 ccgtgataga catgcactct ccttgagttt tatgcaggtg ttaattagcg ggcaattgta
    13201 ccctggttaa gcctcgggtg gcagcatcag gtcttgtatg gaaaagcata tctttgtaaa
    13261 gcggaggtaa ttgctggcct tcaggcgtgg gcaaaccgcg gcaagtggcg atcgagttaa
    13321 tatttgcgta gcgaaaatat ttaaaaattg atttaaatca cgttaaccag gattctcaat
    13381 gcaacttcta aattaatcca gatcaataaa gggtgaatta tcatatgtaa tgtgatctat
    13441 gtaggatcat ttgttactcc aatgtaggta tattcgtcac gtttttataa ccataacgac
    13501 ggagcggata tgaaaaagtt aacagtggcg gctttggcag taacaactct tctctctggc
    13561 agtgcctttg cgcatgaagc aggcgaattt tttatgcgtg caggttctgc aaccgtacgt
    13621 ccaacagaag gtgctggtgg tacgttagga agtctgggtg gattcagcgt gaccaataac
    13681 actcaactgg gcttgacgtt tacttatatg gcgaccgaca acattggtgt ggaattactg
    13741 gcagcgacgc cgttccgcca taaaatcggc acccgggcga ccggcgatat tgcaaccgtt
    13801 caccatctgc cgccaacact gatggcgcag tggtattttg gtgatgccag cagcaaattc
    13861 cgtccttacg ttggggcagg tattaactac accaccttct ttgataatgg atttaacgat
    13921 catggcaaag aggcagggct ttccgatctc agtctgaaag attcctgggg agctgccggg
    13981 caggtggggg ttgattatct gattaaccgt gactggttgg ttaacatgtc agtctggtac
    14041 atggatatcg ataccaccgc caattataag ctgggcggtg cacagcaaca tgatagcgta
    14101 cgcctcgatc cgtgggtgtt tatgttctca gcaggatatc gtttttaatt ccgcacaaaa
    14161 acgaccccgt aatatacggg gtcaataagg acatggcata
//
LOCUS       DAJVUJ010000105        14191 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.105, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000105 DAJVUJ010000000
VERSION     DAJVUJ010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14191
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.105"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            1..960
                     /locus_tag="OQQ48_002828"
                     /pseudo
     CDS             1..960
                     /locus_tag="OQQ48_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
     gene            1131..1478
                     /locus_tag="OQQ48_002829"
     CDS             1131..1478
                     /locus_tag="OQQ48_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HCS1953532.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            1468..1830
                     /locus_tag="OQQ48_002830"
     CDS             1468..1830
                     /locus_tag="OQQ48_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HCS1953533.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            1827..2324
                     /locus_tag="OQQ48_002831"
     CDS             1827..2324
                     /locus_tag="OQQ48_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCS1953534.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGGENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(2332..3516)
                     /gene="emrD"
                     /locus_tag="OQQ48_002832"
     CDS             complement(2332..3516)
                     /gene="emrD"
                     /locus_tag="OQQ48_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HCS1953535.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCVGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFLFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(3935..4024)
                     /gene="tisB"
                     /locus_tag="OQQ48_002833"
     CDS             complement(3935..4024)
                     /gene="tisB"
                     /locus_tag="OQQ48_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HCS1953536.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            4589..4687
                     /gene="ivbL"
                     /locus_tag="OQQ48_002834"
     CDS             4589..4687
                     /gene="ivbL"
                     /locus_tag="OQQ48_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HCS1953537.1"
                     /translation="MTTSMFNAKLLPTALSAAVVVVRVVVVVGNAP"
     gene            4793..6481
                     /gene="ilvB"
                     /locus_tag="OQQ48_002835"
                     /pseudo
     CDS             4793..6481
                     /gene="ilvB"
                     /locus_tag="OQQ48_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418127.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
     gene            6485..6775
                     /gene="ilvN"
                     /locus_tag="OQQ48_002836"
     CDS             6485..6775
                     /gene="ilvN"
                     /locus_tag="OQQ48_002836"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCS1953538.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            6849..7439
                     /gene="uhpA"
                     /locus_tag="OQQ48_002837"
     CDS             6849..7439
                     /gene="uhpA"
                     /locus_tag="OQQ48_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HCS1953539.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            7439..8941
                     /gene="uhpB"
                     /locus_tag="OQQ48_002838"
     CDS             7439..8941
                     /gene="uhpB"
                     /locus_tag="OQQ48_002838"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HCS1953540.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            8951..10270
                     /gene="uhpC"
                     /locus_tag="OQQ48_002839"
     CDS             8951..10270
                     /gene="uhpC"
                     /locus_tag="OQQ48_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HCS1953541.1"
                     /translation="MLPFLKAPADAPLMTDKYEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            10408..11799
                     /gene="uhpT"
                     /locus_tag="OQQ48_002840"
     CDS             10408..11799
                     /gene="uhpT"
                     /locus_tag="OQQ48_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HCS1953542.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(11844..13610)
                     /gene="adeD"
                     /locus_tag="OQQ48_002841"
     CDS             complement(11844..13610)
                     /gene="adeD"
                     /locus_tag="OQQ48_002841"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HCS1953543.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     GGEISGPIVIKGRYIAGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGL
                     GGKELNAYITAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLDL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSHSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVYQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFVFTTLEVTE"
     gene            13785..>14191
                     /gene="adeQ"
                     /locus_tag="OQQ48_002842"
                     /gene_synonym="yicO"
     CDS             13785..>14191
                     /gene="adeQ"
                     /locus_tag="OQQ48_002842"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HCS1953544.1"
                     /translation="MNNDNTDYVSNESGMLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAVGLFLLTLFRIRY"
BASE COUNT         3206 a         3677 c         3944 g         3364 t
ORIGIN      
        1 tgcaacagtc actgaaagac cttgagcggg cttacaaaaa cttcttccag aatcgggctg
       61 cttttccccg attcaaaaag cggggacaga atgatgtatt ccgctacccg cagggcgtta
      121 agctcgatca ggaaaacagc cgtatttttc tgccgaaact gggctggatg cgctaccgga
      181 acagccgtta ggtcacgggt gttgtgaaaa atgtcactgt cagccagtcc tgcggtaagt
      241 ggtacatcag tattcagaca gaaagtgaag tatcaactcc ggttcaccct tcagcatcaa
      301 tggtcgggct ggatgctggc gtggctaaac tcgccacgct gtcagatggc acagtctttg
      361 agcctgtaaa cagttttcag aaaaaccaga agaagctggc gagacttcag cgacagttaa
      421 gccgcaaggt caaattcagc aacaactggc agaagcagaa acgcaaaata cagcgactgc
      481 attcctgtat cgcaaatatc cgcagggact accttcacaa agtcacaacg accgtcagca
      541 aaaaccacgc aatgatagtc attgaggatt tgaaggtcag caacatgtca aagtcagcag
      601 cgggtacggt cagcctgccg gggcgcaatg tccgggcaaa atcaggttta aaccgttcga
      661 tactggatca gggctggtat gaaatgcgcc gccagcttga gtacaagcag ctctggagtg
      721 gcggtcaggt gcttgctgtt ccgccagcgt tgcgcgtgct gtggtcatac agcgaaagaa
      781 aatcgcctgt cacaaagtaa attcagatgc caggtatgtg gatatacagc gaacgccgat
      841 gtaaatggcg ctcgtaacat tttagcggcg gggcacgccg ttcttgcctg tggagagatg
      901 gtgcagtcag gccgcccgtt gaagcaggaa cccaccgaaa tgattcaggc gacagcctga
      961 acgtagcagg gatcctcgtc cttcagggcg tggaggatgt caatttgcgc tatgataccc
     1021 atctgacctc cttgtagcgg atggcaaaag catatcagga aacgggcgga cgaaattctt
     1081 aaaaaaaacc gctaaagata gagcgttagt gaaattgcaa aggagcaaca atgaaaattt
     1141 cccgccttgg agaagcgccc gattaccgct tctcgctggc taatgagcgt acttttctgg
     1201 cgtggatccg taccgcacta ggatttctgg cggcgggggt tgggcttgat caacttgcgc
     1261 cagatttcgc cacgcccgtc attcgtgaac tgctggcgtt gttgttgtgt ctgttttccg
     1321 gcgggttagc aatgtatggc tacctgcgct ggttacgcaa tgaaaaagcg atgcgtctga
     1381 aagaggacct gccctacacc aacagcttat taatcattag cttaatattg atggtcgtgg
     1441 cggtgatcgt tatgggactg gtgttgtatg ccggatagcc gcaaagccag acgcattgcc
     1501 gacccaggac tgcagccgga gcgcacatca ctggcgtggt ttcgtaccat gctgggctac
     1561 ggcgcgttga tggcgttggc tatcaaacac aactggcacc aggcgggcat gttattctgg
     1621 atttcgattg gcattctcgc catcgtggcg ctgatcctct ggcactacac ccgtaatcgc
     1681 aatttaatgg atgtcacgaa tagcgatttt tcccaatttc acgtagtccg tgacaaattt
     1741 ttgatctccc tcgcggtgtt atctctcgca atactgtttg ctgtaacgca tatacatcaa
     1801 cttatcgtat ttattgagag agtcgcatga caggaagtca ggtcattgat gctgaagagg
     1861 acaggcataa actggttgta gagtacaagg atgctctgca acctgccgat ttttatcaca
     1921 atttcaaaca gcggggcatc cgctccgtac agctcattcc ctatcttgaa tttgatgacc
     1981 ggggcgatct gacggccgct tcggtgacag cagagctgtg ggggaaattt ttaattgccc
     2041 tgttcgagtg ttgggtgcgg gcagatatca gtcgtatctc gattgagctt tttgacgcca
     2101 ccttacaaaa atggtgtggc ggcgaaaacc cgcaaccgcg ccgtgactgc caggcgtgcg
     2161 actggcatcg cctgtgcccg cacgctcggc aggagacgcc agacagcgtg ctttgtgcgg
     2221 gctatcaagc gttttattct tactccgcac cgcatatgcg ggtgatgcgt gatctgataa
     2281 agcagcatcg ttctcccatg gagctgatga cgatgctgcg gtgacgtgcg cttaaacggg
     2341 ctgcccctga tgcgacatcc gcgtcgccag cggcagccag cacagcacga tcaacaatcc
     2401 cattaaggtc atcaataacc ccaggctacc ctgaccggtt tgcggcaaca tcgcagagag
     2461 cgacgccagc acgccggaac caatgttttg cagaccgccg accagcgcgc cagccgtgcc
     2521 cgccaggaag aggaacggct ccatcgcgcc gctggtcgcc agcggaaaca gcatcccggc
     2581 accgaaaaag aacagcgcgg cgggaacgag cagcgtccag acattcatca cgccaaacca
     2641 gtcggggatc cacatcagca agccagccag caggcagcag ataaccgact gccacattaa
     2701 cgtggagaag cgtttattgg gacgtccggc aaaccatgcg ccaaaaaacg ctgccggaat
     2761 cggcagaata aacaaaatac tgaccgtcat actgctcagc cctaacaccg cgcccatcag
     2821 cacgccggag caggcttcaa aggcggcaat cccggccaga ccgccaatca gcatcagcaa
     2881 ataacagtta aaaccgctgt taccgaaaag cgttttataa ctggtaagca ggcgcgtgcg
     2941 cggtgcatcg accggacgcg tttccggcat ccagcgggcc atactgaagg tcacaccaac
     3001 acaaagaacc aacaaaaaga gataacaggc gcgccagttc cacatcgtat ccagcagacc
     3061 gccgattagc ggtgcgagca acggactgac gagaatcccc atgtttaaca ggctgttagc
     3121 atggcgcaac tgtgtccgtt catataaatc tcgcggcaaa gtacgcgcca ttacgccgcc
     3181 aacgccggtg cccatcccct gcatcgcgct ggcggcaatc aataccgtca aactggaggt
     3241 cgtgaccgcg accagcgttg ccagcataaa aatggacatt ccgacgagga tcaccggtcg
     3301 gcgacccaca cggtcggaaa tcgggccata aaacagctgt gagacaccgt aagtcagcag
     3361 ataagcgccc attacgctct gcaccgcccc ttcacggacg ttgagatcgc gcgccatatc
     3421 ggcaatagct ggaatataaa tggtttgcgc catctgaccg acggccacga gtaataccaa
     3481 catcaataac aaattgacgt ttctttgcct tttcattatc acggatgctt ttataaaaat
     3541 aattagaata agtcactacg cattcccata aatgcgggag aggaatctat cacattgatg
     3601 aatgttagcc agatatacgc caggaatggg gaattgttta gcggcagaat atgtaaacaa
     3661 aagcggcaat aaatgttgcc gggagggatt acgtgcgaac tgtaaccctc tccccaaagg
     3721 ggcgaggggg aaaccaccgt tccctttcag aaaatgagct aaacattgca cgggacggtc
     3781 ccctcgcccc tttggggaga gggctagggt gaggggcaca ggcagcgcag gtgcaaacat
     3841 tctgctggta gaagaagccc ccaactccaa cccgcacgct aaatataaaa ggggagcggt
     3901 tacccgctcc cctttggtgc gacttgaatc tgaattactt caggtatttc agaacagcat
     3961 caagcagttg cagtgctgca acaatgagtt tgaggataag aatggcgata tccaccaggc
     4021 tcatacgcgt ctcctgtggt tcaggagcac tgccagctga cgtacctttc cgctgcctgt
     4081 tgccagctgt tgtgcacgcg gtatctcaag gagaaacgtt gtgcttgttg acataacaca
     4141 gtgtgctcac gcggggttaa ggcgctgacg gcaccacccg tttcagccag gacttagtgc
     4201 gccgggtaac gatgcggccc ccgcataata cattgcgtac agtgatatta tagaagttta
     4261 ctgtataaat aaacagtgat atttggacaa aacgcaaact gtgatcgata ctcaactatg
     4321 ccctaaatac gtcaaaattc gtgccgaaat tgcgcgttct gcgcggaaca cgtatacttt
     4381 cagtgttgac ataatacagt gtgctttgcg gttaccagct gcaggcggct gatgaaacct
     4441 cgctccggcg gggttttttg ttacctgcaa ttcagtacaa aacgtgatca gcccctcaat
     4501 tttcccttgc tgaaaaattt tccattgtct cccctgtaaa gctgtgcttg tataaatatt
     4561 gttaaacaca aaaccaacaa ggtccccaat gactacttcc atgttcaacg caaaactact
     4621 accaactgcg ctatccgccg cagtggtcgt cgtgcgtgtg gtggtggtcg tcggcaatgc
     4681 gccgtaggga ctggaacaac acacgattcc aaaaccccgc cggcgcaaac cgggcggggt
     4741 ttttcgttta agcacctccc ggaaagtcgg cccagaagaa aaggactgga gcatggcaag
     4801 ttcgggcaca acatcgacgc gtaagcgctt taccggcgca gaatttatcg ttcatttcct
     4861 ggaacagcag ggcattaaga ttgtgacggg cattccgggc ggttctatcc tgcctgttta
     4921 cgatgcctta agccaaagca cgcaaatccg ccatattctg gcccgtcatg aacagggcgc
     4981 gggatttatc gctcagggga tggcgcgcac cgacggtaaa ccggcggtct gtatggcctg
     5041 tagcggaccg ggtgcgacta acctggtgac cgccattgcc gatgcgcggc tggactccat
     5101 cccgctgatt tgcatcaccg gtcaggttcc cgcctcgatg atcggcaccg acgccttcca
     5161 ggaagtggac acctacggca tctctatccc catcaccaaa cacaactatc tggtcagaca
     5221 tatcgaagaa ctcccgcagg tcatgagcga tgccttccgc attgcgcaat caggccgccc
     5281 aggcccggtg tggatagaca ttcctaagga tgtgcaaacg gcagtttttg agattgaaac
     5341 acagcccgct atggcagaaa aagccgccgc ccccgccttt agtgaagaaa gcattcgtga
     5401 cgcagcggcg atgattaacg ctgccaaacg cccggtgctt tatctgggcg gcggtgtgat
     5461 caatgcgccc gcacgggtgc gtgaactggc ggagaaagcg caactgccta ccaccatgac
     5521 tttaatggcg ctgggcatgt tgccaaaagc gcatccgttg tcgctgggta tgctggggat
     5581 gcacggcgtg cgcagcacca actatatttt gcaggaggcg gatttgttga tagtgctcgg
     5641 tgcgcgtttt gatgaccggg cgattggcaa aaccgagcag ttctgtccga atgccaaaat
     5701 tattcatgtc gatatcgatc gcgcagagct gggtaaaatc aagcagccgc atgtggcgat
     5761 tcaggcggat gttgatgacg tgctggcgca attgatcccg caggtggaag cgcaaccgcg
     5821 tgcagagtgg caccagttgg tagcggattt gcagcgcgaa ttcccgtgtc caatcccgaa
     5881 agcgtgcgat ccattaagcc attacggcct gatcaacgcc gttgccgcct gtgtcgatga
     5941 caatgcgatt atcaccaccg atgtgggtca gcatcagatg tggaccgcgc aagcttatcc
     6001 gctcaatcgc ccacgccagt ggctgacctc cggtgggctg ggcacgatgg gttttggcct
     6061 gcctgcggcg attggcgcgg cgctggcgaa cccggatcgc aaagtgttgt gtttctccgg
     6121 cgacggcagc ctgatgatga atattcagga gatggcgacc gccagtgaaa atcagctgga
     6181 tgtcaaaatc attctgatga ataacgaagc gctggggctg gtgcattagc aacagagtct
     6241 gttctacgag caaggcgttt ttgccgccac ctatccgggc aaaatcaact ttatgcagat
     6301 tgccgccgga ttcggcctcg aaacctgtga tttgaataac gaagccgatc cgcaggctgc
     6361 attgcaggaa atcatcaatc gccctggccc ggcgctgatc catgtgcgca ttgatgccga
     6421 agaaaaagtt tacccgatgg tgccgccagg tgcggcgaat actgaaatgg tgggggaata
     6481 agccatgcaa aacacaactc ataacaacgt aattctggag ctcaccgttc gcaaccatcc
     6541 gggcgtaatg acccacgttt gtggcctttt tgcccgccgc gcttttaacg ttgaaggcat
     6601 tctttgtctg ccgattcagg acagcgacaa aagccatatc tggctactgg tcaatgacga
     6661 ccagcgtctg gagcagatga taagccaaat cgataagctg gaagatgttg tgaaagtgca
     6721 gcgtaatcag tccgatccga cgatgtttaa caagatcgcg gtgttttttc agtaaccgct
     6781 caaggcttga acaacatcgc gcttatcgtt aaggtaagcg cgtttttttt acccgccagg
     6841 acaagaccat gatcaccgtt gcccttatag acgatcacct catcgtccgc tccggctttg
     6901 cgcagctgct ggggctggaa cctgatttgc aggtagttgc cgagtttggt tcggggcgcg
     6961 aggcgctggc ggggctgccg gggcgcggtg tgcaggtgtg tatttgcgat atctccatgc
     7021 ccgatatctc cggtctggag ctgctaagcc agctgccgaa aggtatggcg acgattatgc
     7081 tctccgttca cgacagtcct gcgctggttg agcaggcgct taacgcgggg gcacgcggct
     7141 ttctttccaa acgctgtagc ccggatgaac tcattgctgc ggtgcatacg gttgccacgg
     7201 gcggctgtta tctgacgccg gatattgcca ttaaactggc atccggtcgt caggacccgc
     7261 taaccaaacg tgaacgccag gtggcggaaa aactggcgca aggaatggcg gtgaaagaga
     7321 ttgccgccga actgggcttg tcaccgaaaa cggtacacgt ccatcgcgcc aatctgatgg
     7381 aaaaactggg cgtcagtaac gacgtagagc tggcgcgccg catgtttgat ggctggtgat
     7441 gaagacgttg ttctcccgct taattaccgt tattgcctgc ttttttatct tctctgccgc
     7501 atggttttgc ctgtggagta tcagcctgca tctggttgag cgccctgata tggcggtgct
     7561 gttatttccg tttggtctgc gtctggggct aatgctgcaa tgcccgcgcg gatactggcc
     7621 ggtgctgctg ggcgcggagt ggctgctgat ttactggcta acgcaggcgg tcggtttaac
     7681 ccattttccg ttattgatga tcggtagttt actgacgtta ctgcccgtag cgctgatctc
     7741 gcgctatcgc catcagcgtg actggcgcac cttgctgtta cagggggcgg cgttaacggc
     7801 ggcggcgttg ttgcagtcgc tgccctggct ttggcacggc aaagagtcgt ggaatgcgct
     7861 gttgctgact ttaactggcg gcctgacgct ggccccgata tgtctggtgt tctggcacta
     7921 tctcgccaat aacacctggc tgccgctcgg cccgtcactg gtttctcagc caatcaactg
     7981 gcgcgggcga catctggtct ggtacttgct gctgtttgtt atcagtctct ggctccagtt
     8041 gggattgccg gacgaactgt cgcgctttac gccattctgt ctggcgctgc cgattatcgc
     8101 gctggcctgg cactatggtt ggcaaggggc gctgattgcg acgttgatga acgccatcgc
     8161 gctgatcgcc agtcaaacct ggcgcgatca tccggtggat ttattgctct cgctgctggt
     8221 gcaaagtctg acagggttgt tgcttggcgc tggcatccag cggttgcgtg aacttaacca
     8281 gtcgctgcaa aaggaactgg cgcgcaatca gcatctggct gaacggttgc tggaaaccga
     8341 agagagcgtg cgccgtgatg tggcgcgtga gctgcatgat gatatcggtc agaccatcac
     8401 tgctattcgt actcaggcgg gcattgttca gcggctggcg gcagataacg ccagcgtgaa
     8461 gcagagcggg cagctcatcg aacaactatc gctgggcgtt tacgacgcgg tgcgccgttt
     8521 gttgggtcgg ttacgtccgc gccagttgga tgatctcacc ctggagcagg ccatccgctc
     8581 actgatgcgg gaaatggagc tggaagggcg cggtattgtc agccatctcg aatggcgaat
     8641 cgatgaatca gcgttaagcg aaaaccagcg cgtgacgctg tttcgtgtct gccaggaagg
     8701 gctgaacaac attgtgaaac atgctgatgc cagcgcggtc accctgcaag gctggcagca
     8761 ggatgaacgg ttgatgctgg ttattgaaga cgatggcagc ggtttgccgc cgggttccgg
     8821 gcaacaaggt tttggcctca ccggaatgcg cgagcgcgta acggcgctgg gtggcacatt
     8881 acacatttcc tgtctgcacg gcacgcgtgt cagcgtttct ctacctcaac gctatgtcta
     8941 aggtttgatg atgttgccgt ttctgaaagc gcctgccgat gcgccattaa tgactgataa
     9001 atatgaaatt gatgcccgct atcgctactg gcgtcggcat attctgctga ccatctggct
     9061 gggttacgcg ctgttttact tcacgcggaa aagttttaac gccgccgtac cagaaatcct
     9121 tgctaacggc gtgctcagcc gtagcgatat cggcctgtta gcgaccctgt tttacattac
     9181 ctatggcgtg tcgaagtttg tctccggcat tgtcagcgat cgctcaaatg cccgttattt
     9241 tatggggata gggcttatcg ccacgggcat tatcaacatt ctgtttggct tctcgacgtc
     9301 gctatgggcg tttgccgtac tctgggtgct gaacgccttt ttccagggct ggggttcacc
     9361 ggtgtgtgcg cgtctgttaa cggcctggta ttcacgtacc gagcgcggtg gttggtgggc
     9421 attatggaac acggcgcata acgtcggcgg cgcactcatt cccattgtga tggcagcggc
     9481 tgcactacat tacggctggc gtgccgggat gatgattgct ggttgtatgg cgatcgtcgt
     9541 gggaattttt ctctgctggc ggctgcgcga tcgcccgcag gcgttaggtt taccggcggt
     9601 cggtgaatgg cgacacgacg cgctggagat agctcaacaa caagaagggg cagggctgac
     9661 gcgtaaagag atcctcacca aatatgtgtt gctgaatccg tatatctggc tgctttcgtt
     9721 ttgctatgtg ctggtctatg tggtccgggc ggcgatcaac gactggggca atttgtatat
     9781 gtccgagacg ctgggcgtcg atctggtcac ggcgaatacg gcagtgacga tgtttgaact
     9841 gggtggattt atcggtgcgc tggtagcggg ttggggctcg gacaaattgt ttaacggcaa
     9901 ccgagggccg atgaatttga ttttcgccgc cggaattttg ctttcagtcg gctccctgtg
     9961 gctgatgcca tttgccagct acgtgatgca ggcaacctgc ttcttcacca ttggtttttt
    10021 tgtctttggc ccacagatgt taatcggtat ggcggcggca gagtgttccc acaaagaggc
    10081 ggcaggggcg gcgacggggt ttgtcggctt gtttgcttat ctgggggcgt cgcttgctgg
    10141 ttggccgctg gcgaaagtac tcgatacctg gcactggagc ggattttttg tggttatctc
    10201 tatcgccgcc gggatttccg cactgctgtt actgcccttt ttgaacgccc agacaccgcg
    10261 cgaagcgtga tgcatctcac cttttcactt catatccggc aaaactaaga aattttccag
    10321 gttttgcctg gacgctatct caggcctgat ttgctgctga tttttacaat gcatgcctca
    10381 cgcaggtatt catttcagga gtaacccatg ctggctttcc taaaccaggt tcgcaagccg
    10441 accctggacc ttccgctcga agtgcggcgc aaaatgtggt tcaaaccgtt catgcaatcc
    10501 tacctggtgg tctttatcgg ctacctgacg atgtacctga ttcgcaagaa ctttaacatc
    10561 gcgcagaacg atatgatttc gacctacggg ttgagcatga cgcagctggg gatgatcggc
    10621 ctgggtttct ccatcactta tggcgtgggt aaaacgttgg tttcatacta cgccgacggc
    10681 aaaaacacca aacaattcct gccgttcatg ctgatcctgt ctgccatttg tatgctgggc
    10741 ttcagtgcca gtatgggcag cggctcggtt agcctgttcc tgatgattgc cttctacgcc
    10801 ttaagcggct ttttccagag taccggcggt tcgtgcagtt actccaccat caccaaatgg
    10861 acgccgcgtc gtaaacgcgg gacattcctc ggtttctgga atatttctca caaccttggc
    10921 ggtgcaggcg cagcaggtgt ggcgctgttc ggggcaaatt acctgttcga tggccatgtc
    10981 atcggcatgt ttatcttccc gtcgattatc gcgctgattg tcggttttat cggcctgcgt
    11041 tacggcagcg actccccaga atcttatggc ctcggcaaag ctgaagaact gttcggcgag
    11101 gagatcagcg aagaggacaa agagacagaa tctaccgata tgaccaagtg gcagatcttt
    11161 gttgagtatg tgctgaaaaa caaagtgatc tggctgctgt gcttcgccaa cattttcctc
    11221 tatgtggtac gtattggtat cgaccagtgg tcaaccgtat acgcgttcca ggaactgaaa
    11281 ctctctaaag cggtggcgat tcagggcttt acgctgtttg aagctggtgc gctggtcggt
    11341 acgctgctgt ggggctggct ctctgacctg gcgaacggtc gccgtggcct ggtggcctgc
    11401 atcgcgctgg cgctgattat cgccacgctc ggtgtgtatc aacatgccag caacgaatat
    11461 atctatctgg cttctctctt tgcgttgggt ttcctggtct ttggcccgca attgttgatt
    11521 ggtgtggctg ctgttggctt tgtacctaaa aaagcgattg gcgctgccga tggtattaaa
    11581 ggcacctttg cttacctgat tggtgacagc tttgccaagt taggtctggg aatgattgcc
    11641 gatgggacgc cggtattcgg ccttaccggc tgggcaggca ccttcgccgc acttgatatc
    11701 gccgcgattg gttgtatctg cctgatggcg atagtggcgg tgatggaaga acgcaaaatc
    11761 cgccgcgaga aaaaaattca gcagttgaca gtggcataaa cgtaactggt gacttttgcc
    11821 cggcatgacg ccgggctttt ttattattcc gtgacttcca gcgtagtgaa gacaaacttc
    11881 tcgccatcaa atagcccctg actggttagt ttgagcgcgg ggatcactgg cagagaaaga
    11941 aacgccatct gaataaacgg ctcatcgggt aacggaccgc attcacgggc ggcggctttc
    12001 aaggcgtcaa tttgttccgc cagcgactgc gccgtgtcgg tgctcatcag gccggcaatg
    12061 ggcaacggca gatgactttg gacctggccg ttacgtacca cgcacagccc gccgccatcc
    12121 tgaatcacct gatagaccgc cagcgccatc tcttcggcac tgcgaccgat caccacaata
    12181 ttatggctgt catggctgac cgtcgcagcc agcgcacctt cattcagtcc aaagccgcca
    12241 agcaaaccac aagccggagc cagccgttgc ccgtaacgct caagtacggc aataaagcac
    12301 acatcatcgc gatcaaaacc attttcgctg tagacgctgg agtgggagtg cgtaatcaat
    12361 tcgttatgga tgacgtcaat gacccgatag cgttttccgg gcgtaaattg cagggcaaag
    12421 tcgctggcgg aaactggctg gcgggcaatg gtgttgccat atggcggagc ggattgtgcc
    12481 agtctcgccg actcttccgc ctgtaaggtt tgcgcatcaa tcggctcgcc tttcaccagt
    12541 acctgctgca ccgtgacctt acgcgcatcg ctcaacagga cgatatcggc ctgcttgccg
    12601 ggtgccagta agtcgaggtg attcagacca aagtggcgcg ccgtcgacca gctggcgacg
    12661 cgatatgcca catgcagcgg cacattgtgt tgttcgatca ggcggcgaat taaggcatcg
    12721 atgtgtcctt catgggcgat ctcccacggg ttacggtcat cggtacagag catgcattgc
    12781 gggctgttaa attcgttgat caacggtgcc agcgcgttga gattgcgggc agcggaccct
    12841 tcgcggatca tcaacgacat gccgagttgt aatttccggc gtccttcttc cagctgataa
    12901 ctttcgtggc agttttcaat acccgcagta atataggcgt taagttcttt accacccaaa
    12961 cccgggcagt gaccgtccag cgtcaggtgg cgaaatgcat ccagtttatc gagcagcgca
    13021 ttctgcccgc taattacgcc agggtagtcc atcatttctg caaggccggt aacctgcgga
    13081 tggtcccgcc aggcgagcat ctgttcaagg gtaaaactgg caccgttaac atcgcagcct
    13141 tccagggcgg gtacgcaaga gctgacctgt aagtactggt tttgccttgc ctgttcggca
    13201 cagcgggcaa accaggcgaa tccggcttct cccatcacgt tgacgatttc atgagggtcg
    13261 caaataacgg tcgtcaggcc gcgcggcagg gtagcggttt caaaggtgac cggcgtcatc
    13321 atgctggatt caatatgcag gtgagcatca ataaaccctg gcaccgccgt tgcgccgcga
    13381 gcatcaatcc gctgcaaagc cggagcatca gcgtattctg cgccaacacc ggcaatgtaa
    13441 cgtcctttaa tcacaattgg gccggaaatt tctccgccat tgatcaggtc gagaatagag
    13501 acattatcaa taatataatc ggcaacagcg tcgccacggg aaacggctaa caattcctgg
    13561 tattcagccc ggctaatgtg atgaaattta tggttaatgg aattattcat atttttctca
    13621 ttttgatttt atcaatttga ttattattta tcataaagaa acgtcgcgag attattaaag
    13681 aatatctatt aatgtgcaat tgaaatgtga taattatcac tgaaccgttg ttgtcatttg
    13741 ttttacaaaa gcaagggatt aaatctcctc caatggacaa aaaaatgaat aatgacaata
    13801 ccgattacgt gagtaatgaa tcagggatgc tttcgcgatt atttaaacta cctcagcatg
    13861 ggaccaccgt ccgcacagaa ttgattgcgg ggatgaccac ttttttaacc atggtgtaca
    13921 tcgtttttgt gaacccgcaa atcctcggcg cggcacaaat ggacccgaaa gtggtgtttg
    13981 ttaccacctg tttgattgcc ggtatcggca gtattgcgat ggggatattt gctaacttac
    14041 ccgtggcgct ggctccggca atggggctga acgccttctt tgccttcgtg gtcgtggggg
    14101 cgatgggcat ctcctggcag accgggatgg gcgcaatatt ctggggcgca gttggactat
    14161 ttttgctcac gctgtttcgt atccggtact g
//
LOCUS       DAJVUJ010000106        14122 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.106, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000106 DAJVUJ010000000
VERSION     DAJVUJ010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14122
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.106"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(<1..352)
                     /locus_tag="OQQ48_002843"
     CDS             complement(<1..352)
                     /locus_tag="OQQ48_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCS1953545.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIDTL
                     NIGGMAWRPGKKQLT"
     gene            complement(352..759)
                     /locus_tag="OQQ48_002844"
     CDS             complement(352..759)
                     /locus_tag="OQQ48_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HCS1953546.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGDARVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATAEEDF"
     gene            complement(769..1575)
                     /locus_tag="OQQ48_002845"
     CDS             complement(769..1575)
                     /locus_tag="OQQ48_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCS1953547.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALVWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            complement(1645..2592)
                     /locus_tag="OQQ48_002846"
     CDS             complement(1645..2592)
                     /locus_tag="OQQ48_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HCS1953548.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGIVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDVALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIVGALRGKYINCLVTNSSTAELLLK"
     gene            2940..3812
                     /gene="rluF"
                     /locus_tag="OQQ48_002847"
     CDS             2940..3812
                     /gene="rluF"
                     /locus_tag="OQQ48_002847"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HCS1953549.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            complement(3945..4217)
                     /locus_tag="OQQ48_002848"
     CDS             complement(3945..4217)
                     /locus_tag="OQQ48_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HCS1953550.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(4469..5818)
                     /gene="lysC"
                     /locus_tag="OQQ48_002849"
     CDS             complement(4469..5818)
                     /gene="lysC"
                     /locus_tag="OQQ48_002849"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HCS1953551.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNDGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            6343..7992
                     /gene="pgi"
                     /locus_tag="OQQ48_002850"
     CDS             6343..7992
                     /gene="pgi"
                     /locus_tag="OQQ48_002850"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCS1953552.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            8454..8696
                     /locus_tag="OQQ48_002851"
     CDS             8454..8696
                     /locus_tag="OQQ48_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1953553.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            8810..9448
                     /locus_tag="OQQ48_002852"
     CDS             8810..9448
                     /locus_tag="OQQ48_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HCS1953554.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKILLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            9445..10182
                     /locus_tag="OQQ48_002853"
                     /pseudo
     CDS             9445..10182
                     /locus_tag="OQQ48_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418452.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
     gene            10182..12278
                     /locus_tag="OQQ48_002854"
     CDS             10182..12278
                     /locus_tag="OQQ48_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCS1953555.1"
                     /translation="MKKRHLLSLLALGISMACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNVGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETKLAQKRVEPVVPQSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     MADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVLKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(12325..12603)
                     /locus_tag="OQQ48_002855"
     CDS             complement(12325..12603)
                     /locus_tag="OQQ48_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1953556.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            12604..12747
                     /locus_tag="OQQ48_002856"
     CDS             12604..12747
                     /locus_tag="OQQ48_002856"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1953557.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPAWVRFVLPDAPPGAAYP
                     AYG"
     gene            12873..13271
                     /gene="psiE"
                     /locus_tag="OQQ48_002857"
     CDS             12873..13271
                     /gene="psiE"
                     /locus_tag="OQQ48_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HCS1953558.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLIIVDHK
                     SPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(13315..>14122)
                     /locus_tag="OQQ48_002858"
     CDS             complement(13315..>14122)
                     /locus_tag="OQQ48_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HCS1953559.1"
                     /translation="PRWLMSRGKQEQAESILRKIMGNTLATQAVQEIKHSLDHGRKTG
                     GRLLMFGVGVIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVIN
                     LTFTVLAIMTVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAF
                     AMSWGPVCWVLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHN
                     GFSYWIYGCMGVLAALFMWKFAPETKGKTLEELEALWEPETKKTQQTATL"
BASE COUNT         3417 a         3661 c         3632 g         3412 t
ORIGIN      
        1 tggttaactg ttttttaccg ggccgccagg ccatgccgcc aatatttagg gtgtctattt
       61 ttaccccttg gcgaaccatc gctaaggcat cctgtgggcg agtgaaaaga taaaaaacgg
      121 tttcgtcctg atattgcggg ttgtgataaa cagcgacggc tttttcaata ttgaccacgt
      181 taaccttcat tcccggtggc gcagcctgac gcaataatgt ccggcgaact tcatcgttat
      241 aaacttcgtc attgcagata ataatgcgct gggcgttagc cactttcgac cagacggtag
      301 tgacctgacc gtggatcagg cggtcatcaa tgcgggcgag cgtaatgttc atcagaaatc
      361 ctcctctgcc gttgccagtt gtttccagac aacgcaggtt tgctttgcga tttgcgtcag
      421 gtgttcacac agttcatcaa cattcatgct gtcctggtta tcaaccagtt cgagcgccag
      481 cggcagagaa aggccgctga ttacccgcag acgaggattg cgcatcgcaa gcatcgctgc
      541 cgcattccac gggctgccgc actgtaaatc cacggcaatc agccactcat cttgattgtg
      601 aatgcttact aacttttcca gctttgctac gatgtctccg gcgttctcgc cgggcacaaa
      661 ttcaactgcc tcgaccctgg cgtcgccgta caccatctga accgattcca gcatggcgca
      721 agctaatttg ccgtgggcgc aaaaaatggc attgaccata aagcctcctt agccgcgcgt
      781 tttgccgccc gcaatgttag tggttactcc ggtgatatag ctggcgcgtt cagacagcag
      841 ataacaaaca aaatcagcga cttctgctaa tcttccggcg cgcccaatag gaatggcgtt
      901 tttggtatag ccttcacgca gctgctcgac ggtgatattg cgcgtccaca ccagcgcttc
      961 ttcatattcc ggcgtgcgca gtcctgtttt ttccagaatc cccggcgcga taccgaccac
     1021 acggataccg tgtttaccca attctttcga ccaggatcgc gtgaagctat taagcgcggc
     1081 tttggtagcg gcgtaacagc tttggccttc tgagccttcc agtccacttt ctgaggaaac
     1141 attcacaatc acgccatcat gttgtttgac catctgtcgc gccaccgcct gcgacatcag
     1201 aaaaacgcct ttctggttga tattgaccat tttttcgaat gcagcttcgt tgagttcata
     1261 ctgcccggca ggcgctttct catcgaccag cagacgcggg aaattgaccc cggcgttatt
     1321 gaccagaccg tcgatgcgac caaaacgctg gataatttcc gctaccgtat gatttacctc
     1381 tttggcgctg gaaatatcgg tcggccaaaa ctgatagcct ttatgacctt catattgacc
     1441 atcgccaccg tgaatatcta ccatctgtac gtttgcgcct tgtgctaata attcctctac
     1501 aatcgccaga ccaataccgg atgcgccgcc ggtgacaata atgattttat cctgcaaatt
     1561 taaccacgtt tgcataacag actcctgatt ttagataata gggccccgcc gtaattttta
     1621 ttacgctaaa catcgggaag atttttattt cagtaatagt tcagctgtgc tgctattcgt
     1681 taccagacaa ttaatatatt ttccacgcag tgcaccaaca attccgctgt atttttcttc
     1741 actcatggca atgccaatgg aatatcgtgc ctgctttaat ttattcattt cgatagagag
     1801 tgttttttcg ctcatattcg tttcaaccat tgcgccgtga atatcaaaaa agcgcgagca
     1861 aatatcgcca gcaacctggc gggcattcag gtcgtcactc tcttcaccac cataaaacgc
     1921 atgccagtta gcgccgtcgc gaatggccgg tgagccaatt cccaccaggg cgacatccag
     1981 attatcccag taggcagaga tggttttaaa gtgctgagac tgcatgatcc cattacgaat
     2041 taatgggtta tccagcagag ccggaaaatc cgcgagatgc gattcccctt tcagcttcgc
     2101 tgccgcgctg taggttaatg tgtttacatg atagcggctt tcgagtttac cggacggtcc
     2161 gccaataatt ggtacgcaga ttaactgccg cgattgcccc gcctgcggca agttttcaac
     2221 cagtgcgctc accgcgcgac cccaggaaaa accgacaata tcgccaggct ccagcaagcg
     2281 atccagcagt tgcgcgccgt gtaaccccat catcgccagt tgagtctctt catcctcatc
     2341 atttcccgac accaccacaa cgtctttcag gccaaacttt tgcttcagtt gctgctccag
     2401 ccagagattt tcgttgtagt catagttgat ggcgatggtg acaattccct gatcgcggcc
     2461 tcgtttaagc aagcggctga tggtggtgcg gtaaataccc agttcgcgcg cgatttgcgc
     2521 ctgcgtcata tcctgttcgt aatagagttg ggcaatcttc acaatcaaac ggatatcgtc
     2581 actgttttcc attgccgcct cctggttctg cacatttgtg caaatacgct ttctcaccgc
     2641 ttgatctgat ataaccccat catgagggcc tttcaaagcg atttttcact ctttgcatgg
     2701 ggagatatag cgctttctct tgcacatttg tttctctgcg tagtagtcat cagcacaggt
     2761 gtgcagcgat tcgtaatatg ttgattatcc ttattttgcc aggcaaattt gcatcgtact
     2821 tccggagaca cttttgtgat aaaaggcgtg gcagatcaca tttaatttcg acgttgccgc
     2881 ataaccctgt ttttccccgc taagaggtac ggtatattcc gcgttttaca ggagaattta
     2941 tgctgcccga ctcatcagtc cgtttaaata aatacatcag cgaaagcgga atttgctcac
     3001 gccgcgaagc ggatcgctat atcgagcaag gcaatgtgtt ccttaatggc aagcgagcca
     3061 ccattggcga tcaggtgaaa cccggcgaca ttgtgaaagt aaacggtcag ttgattgagc
     3121 ctcgggaagc cgaagatttg gtacttatcg ccctgaacaa gcccgtcggt attgtcagca
     3181 ccaccgaaga tggcgagcgc gataacattg tcgatttcgt taaccacagc aaacgcgtgt
     3241 tcccgattgg ccgcctggat aaagactccc aggggctgat tttcctcacc aatcacggcg
     3301 atctggtgaa taagatcctc cgtgcgggta acgatcatga gaaagagtat ctggtgacgg
     3361 tcgataaacc gattaccgat gaatttattc gtggtatggg ggcgggggtg ccgatcctcg
     3421 ggacggtgac gaaaaagtgc aaagtcaaaa aagaagcgcc gtttgtcttc cgcattaccc
     3481 tggtgcaggg gctgaaccgt cagatccggc gcatgtgcga gcatttcggc tatgaagtga
     3541 aaaagctgga acgtacgcgc attatgaacg tcagcttaag cggcattccg ctgggggagt
     3601 ggcgcgattt aaccgacgat gagttaatcg acctctttaa gctcatcgaa aattcctctt
     3661 ccgaggcaaa acctaaagcg aaggccaaac cgaaaacagc gggcatcaaa cgtccagtcg
     3721 ttaagatgga aaaaacggcg gaaaaaggcg gtcgcccggc gtccaacggt aagcgtttta
     3781 cctcgccggg gcgtaaaaag aagggacgct gattaacgcc tcagactgat gaaaacgtcc
     3841 aaaaagagct cggacagtcc cctcgcccct tcggggagag ggttagggtg aggggaagat
     3901 tgtgtccaca cagacgagtg aaaatctcag gcgttgattg ttcattagcg cctgccgcgc
     3961 gtcgacgtat ttgctgacca gctaaatgac gcttcaggat ccggtttata agcctgtttt
     4021 ttcttcaatt ggcgggcttt tttcgcttca gcctcacgct cgaccattaa tttatcgatg
     4081 tactctttct taatgctgtt ggtttcggag ttggtcagta cgcgaccatg cgcaatccgc
     4141 gcccgatcca gcagtgtttt aagttcacgc tgctcgcgtt cagtcatctc tttttgggta
     4201 atacggggga gtgccatgat ggtgccctca gtgagccaga gagttcagtg tacgggaatt
     4261 cgtttgcgag cagaactgat aatattcatt ttgccttctc ttaactattt atctctttcg
     4321 ataattaatt agagatgcag ataaaaaaat taaaggcaat tattctcgat aaagacgttg
     4381 gttaacagag tacaggctcg tttattgata agcatagtga caagaaaatc aatacggccc
     4441 gaaatatagc ttccaggcca tacagtattt actcaaacaa attactatgc agtttttgca
     4501 ccacctgctc ggcatcttcg ccgggcacca ggaagcacag gttatggctg gatgcgccgt
     4561 aacaaatcat gcgaatgttg aacggttcca gtacgccgaa tacctctttg ccaacgccgc
     4621 aggcttttga caggtcattg ccaatcaacg cgaccagcgc cagaccttct tctacttcca
     4681 cccggcacag tgcggaaagc tccatcagca gagattgcgt cagcaacgta tcgccagtgg
     4741 aggttgaacc ggtggtatca agggttaatg ccacgctcac ttctgacgtg gtgattaagt
     4801 ctaccgaaat attatgccgc gcgaggatgc cgaaaacttc cgcgaggaag ccgcgagaat
     4861 gcagcatatt caggctgtgc aaagtgagca gagtctgatt gcgacgaagc gccagcgcgc
     4921 ggaacagcgg aggattttca gttttattgc acaccagcgt accacctgcg cgtgggtctt
     4981 tgctggagcc gacaaagacc gggatatcgc tgcgtactgc gggtagcaac gttgccggat
     5041 gcagtacttt tgcaccaaaa gttgccatct ctgccgcttc ggcaaacgcg atttcatcaa
     5101 tgcgttttgc tgcggaaact acgcgtggat cggtggtgta gatgcccggg acgtcggtcc
     5161 agatatcaac acgagatgcg tgtaaagcct ccgccagcaa ggctgccgta taatcgctgc
     5221 ctccacggcc aagcgtcgtt gtacgacctt tattttcgct accgataaat ccctgggtga
     5281 tcactaagcc gtcattgagg cgtgggagca gctgcagcgc ggccagttcc gccagcgcgg
     5341 ctacatctgg ctctgcacga ccaaatcggt cgttggtacg catcacttta cgtacatcaa
     5401 accactgtgc ctgaacatcg cgttcgcgca ggatttcgac aaacagcagg gtcgacatca
     5461 gctcgccgtg gctgaccagc tcatctgtca gcgccggaga cgttgccagc gccgccgctt
     5521 ctgccagaac agtaatgttc tccagcagac gttcaatctc ttcacggata acgttcgggt
     5581 aacgcagacg ttccagaatg gcaaactgga tgttgcgaat agcgtcgagt ttttcgaatc
     5641 gctcgccagg ttccagccct tccgctaaag cgaccagcag attagtgata ccagcagaag
     5701 ccgagaggac tactaaacgc acgttggcat cagaaagcac aatatcagcg ctgcggttca
     5761 tggcgtcaaa atcagctacg ctggtaccgc caaatttgga gacaacaatt tcagacataa
     5821 ctacctcgtg tcaggggatc cattttcagc cttggcacaa gggaagagcg gaagacgggt
     5881 gggcgcagag cgatacttcg ctactatttt cacccagaag tgctccacca cttgcgaaac
     5941 gcccgactgc gaacgcttct ggtgacaacc caggggattc agcccctgta gccgatgatg
     6001 aacgtggcca gccgttcaat cacctcggcg atgcaccccc tcaggtgtta tcacaggact
     6061 ggctcctcca acaccgttac ttgggcaacg cgcctcttct ggcctgcgct agcgcaggta
     6121 gtacatttat aaataaaggg tgagcggggc ggttgtcaac gacggggtca tgcggatttt
     6181 tcatccactc ctggcggtca gtagttcagc taataaatgc ttcactgcgc taagggttta
     6241 cactcaacat tacgctaacg gcactaaaac catcacattt ttctgtgact ggcgctacaa
     6301 tcttccaaag tcacaattct caaaatcaga agagtattgc taatgaaaaa catcaatcca
     6361 acgcagaccg ctgcctggca ggcactacag aaacacttcg atgaaatgaa agacgttacg
     6421 atcgccgatc tttttgctaa agacggcgat cgtttttcta agttctccgc aacattcgac
     6481 gatcagatgc tggtggatta ctccaaaaac cgcatcactg aagagacgct ggcgaaatta
     6541 caggatctgg cgaaagagtg cgatctggcg ggcgcgatta agtcgatgtt ctctggcgag
     6601 aagatcaacc gcactgaaaa ccgcgccgtg ctgcacgtag cgctgcgtaa ccgtagcaat
     6661 accccgattc tggttgatgg caaagacgta atgccggaag tcaacgcggt gctggagaag
     6721 atgaaaacct tctcggaagc gattatctcc ggtgagtgga agggttatac cggcaaagca
     6781 atcactgacg tagtgaacat cgggatcggt ggttctgacc tcggcccgta catggtgacc
     6841 gaagcactgc gtccgtacaa aaatcacctg aacatgcact ttgtttctaa cgtcgatggg
     6901 actcacatcg ctgaagtgct gaaaaaagta aacccggaaa ccacgctgtt cttggtagca
     6961 tctaaaacct tcaccactca ggaaactatg accaacgccc atagcgcgcg tgactggttc
     7021 ctgaaagcgg caggtgatga aaaacacgtt gcaaaacact ttgcggcgct ttccaccaat
     7081 gccaaagccg ttggcgagtt tggtattgat actgccaaca tgttcgagtt ctgggactgg
     7141 gtcggcggcc gttactcttt gtggtcagcg attggcctgt cgattgttct ctccatcggc
     7201 tttgataact tcgttgaact gctttctggc gcacacgcga tggacaagca tttctccacc
     7261 acgcctgccg agaaaaacct gcctgtcctg ctggcgctga ttggcatctg gtacaacaat
     7321 ttctttggtg cggaaactga agcgattctg ccgtatgacc agtatatgca ccgtttcgcg
     7381 gcgtacttcc agcagggcaa tatggagtcc aacggtaagt atgttgaccg taacggtaac
     7441 gttgtggatt accagactgg cccgattatc tggggtgaac caggcactaa cggtcagcac
     7501 gcgttctacc agctgatcca ccagggaacc aaaatggtac cgtgcgattt catcgccccg
     7561 gctatcaccc ataacccgct ctctgatcac caccagaaac tgctgtctaa cttcttcgcc
     7621 cagaccgaag cgctggcgtt tggtaaatcc cgcgaagtgg ttgagcagga atatcgcgat
     7681 cagggtaaag atccggcaac gcttgactac gtggtgccgt tcaaagtgtt cgaaggtaac
     7741 cgcccgacca actccatcct gctgcgcgaa atcactccgt tcagcctggg ggcgttgatt
     7801 gcgctgtatg agcacaaaat ctttactcag ggcgtgatcc tgaacatctt taccttcgac
     7861 cagtggggcg tggagttggg taaacaactg gcgaaccgta ttctgccaga gctgaaagat
     7921 gacaaagaaa tcagcagcca cgatagctcg accaatggtc tgattaaccg ctataaagcg
     7981 tggcgcggtt aatcatcatg cgtgcctgat gccgatatgt aggccggata aggcgtttac
     8041 gccgcatccg gcaaccggag cctgatgcca atatcgacat taatgcatag tatgaggagt
     8101 taaataacct taaaaacaat gccgacaata agtcggcatt gttttatcag ataaatcccc
     8161 ttgtctgtga tttaacggaa atcataccgt gaggttaatc ctaaaataga tttttaatcg
     8221 ttgtttattt cggagaatac gcagattaat tacttttgtt tttattttaa gtttatgatt
     8281 tttattgtta tttaaatata agttgaagct tatatttgat attcattcca attatcctaa
     8341 aacgccatcg ctaattcccc gcgccgtaat tcgcatgctt tagttgtgta tactcgatcc
     8401 cgcccgaaat gtttttgggt aaatctccat tcattcaatg aagggaaatt gttatgaaaa
     8461 aagttctgta tggcattttt gccatatctg cgcttgcggc gacttctgcg tgggctgcac
     8521 ctgtacaggt gggcgaagcg gcagggtcgg cagcaacgtc ggtttcggcg ggtagttcct
     8581 ccgcgaccag cgtcagcacc gtaagctcgg cggtgggtgt cgcgctcgcg gcaaccggtg
     8641 gcggtgatgg ttctaatacc gggaccacca caaccacgac caccagtacc cagtaataaa
     8701 gtatgtatcc ccaaaataat ttgagtcatt gcatctgtgg cttgaagtat gaagggattt
     8761 accataacca cactccggtg tggttattct gcccctctgg agaagagtcg tgaagcgacc
     8821 tgcactcatt cttatctgcc tgctattaca ggcctgttca gccacgacta aagagctggg
     8881 caattcactg tgggacagtc tgttcggcac gccaggcgta cagctgacgg acgatgatat
     8941 tcaaaatatg ccctacgcca gccagtacat gcagcttaat ggcgggccgc aattgtttgt
     9001 ggtgctggcc ttcgctgaag acggacaaca aaaatgggtc actcaggatc aagccactct
     9061 tgtcacacaa catggtcgtc tggtgaagat tttgcttggc ggcgacaacc tgattgaagt
     9121 gaataacctt gccgccgacc cgctgattaa acccgcacaa attgttgatg gtgcaacatg
     9181 gacgcgcacg atgggctgga ccgagtacca gcaggtacgc tacgccaccg cacgctcagt
     9241 cttcaaatgg gatggcaccg ataccgtcaa agtcggcagc gatgaaaccc tggttcgcgt
     9301 gctggacgaa gaagtctcca ccgaccaggc gcgctggcat aaccgctact ggatcgacag
     9361 cgaagggcaa attcgccagt cggaacagta tctcggcgcg gattatttcc cggtgaaaac
     9421 cactctcatc aaggcggcaa aacaatgatt aaacaaactt ttgtcgcgtt gcttttgagt
     9481 gtgggagcgt catcggtctt cgcggcagga accgtcaagg tgttcagcaa tggcagcagt
     9541 gaggccaaaa cactaacagg cgcagagcat ttaatcgatc tggtaggcta accgcggctg
     9601 gcaaacaact ggtggcccgg tgcggtgatt agcgaagagc tggcaacggc ggcagcattg
     9661 cgtcagcagc aggcgttgct gacacggctg gctgaacagg ccgcagactc cagcgccgac
     9721 gatgccgccg ccattaacgc cttacgccag caaattcagg cgttgaaagt gacgggcagg
     9781 caaaaaatca atcttgatcc cgatatcgtg cgcgttgccg aacgcggtaa cccaccgttg
     9841 cagggcaact acacgctgtg ggtcggacca ccgccgtcca cggtcacgtt gttcgggctt
     9901 atcagccgtc ctggcaagca gccattcact ccaggtcgcg acgtggcgag ctatctctcc
     9961 gggcagaacc tgcttagcgg tgcggatcgc agctacgcgt gggtggttta cccggacgga
    10021 cgcacgcaaa aagcgccggt ggcttactgg aacaagcgtc acgtagagcc gatgcccggc
    10081 agcattattt atgttggcct cgcggactcc gtctggagtg agacgcctga tgcccttaac
    10141 gccgacattc ttcagactct gacgcagcgg ataccccaat aatgaaaaaa agacatctgc
    10201 ttagcttact ggcgctgggc attagcatgg cttgctacgg cgaaacatat cctgcgccca
    10261 ttggcccgtc gcagtcggat ttcggtggcg taggattatt acaaacgccc accgcacgca
    10321 tggcgcggga aggggagttg agcctgaact atcgcgataa cgatcagtac cgttattact
    10381 cagcttcagt gcaactcttc ccgtggctgg aaacaacgct gcgctacacc gacgtgcgca
    10441 cccggcagta cagcagcgtc gaagcgttct ctggcgatca aacgtataaa gataaagcct
    10501 tcgatctcaa actgcgtttg tgggaagaga gttactggct gccgcaagtg gcggttggtg
    10561 cgagggatat cggcggtacg gggctgtttg atgcggaata tcttgttgcc agcaaagcct
    10621 gggggccgtt cgattttacg ctcggcctgg gctggggtta tctgggcacc agcggtaatg
    10681 tgaaaaatcc gctctgttca gccagtgata aatattgcta tcgcgataac agctacaaac
    10741 aggcgggatc tatcgacggt agccagatgt tccacggtcc tgcctcactg tttggcggcg
    10801 tggaatacca gacgccctgg caaccgctgc gcctgaaact ggagtatgaa ggcaataatt
    10861 atcagcagga ttttgccggg aagctggagc aaaaaagtaa gtttaacgtc ggtgcgattt
    10921 atcgcgttac cgattgggcc gacgttaacc ttagctatga acgtggcaac acctttatgt
    10981 ttggcgtcac gctgcgcacc aactttaacg atctgcgccc gtcttacaac gataacgccc
    11041 gcccgcaata tcaaccgcag ccgcaggatg ccattttgca gcattcggtg gtggcgaatc
    11101 agttaacgct gttgaaatac aatgtcggac ttgccgatcc gcagatccag gcgaaaggcg
    11161 atacgctgta tgtcaccggc gagcaggtga aatatcgtga ttcgcgcgaa gggatcatcc
    11221 gtgctaatcg gatcgtgatg aacgatctgc cggatgggat caaaacgatc cgcattacgg
    11281 aaaatcgtct taacatgccg caggtgacga cggaaaccga tgtcgccagc ctgaaaaatc
    11341 atctcgccgg agagccgttg ggccacgaaa cgaagctggc gcaaaaacgc gtcgagccag
    11401 tggttccgca gtccaccgag cagggctggt atatcgacaa atcacgcttt gatttccata
    11461 tcgatccggt gctgaaccag tcggtcggtg gcccggaaaa cttttacatg tatcagctgg
    11521 gcgtgatggg aatggcagat ttgtggctga cggaccatct gctgaccacc ggcagcctgt
    11581 ttgcaaatct tgccaacaac tacgacaagt ttaactacac caatccgccg caggactcgc
    11641 acttaccgcg cgttcgtacc catgtgcgcg agtatgtgca gaacgatgtc tatgtgaata
    11701 acctgcaagc caactacttc cagcatctgg gcaacggctt ctacggtcag gtctatggag
    11761 gttatctcga aaccatgttt ggcggtgcgg gggccgaagt gttgtatcgt ccgctggaca
    11821 gtaactgggc gtttggtctg gatgccaact acgttaaaca gcgcgactgg cgtagtgcaa
    11881 aagatatgat gaaattcacc gactacagcg tgaaaaccgg acatctgacc gcctactgga
    11941 cgccatcttt cgcccaggac gtgttggtga aagccagcgt cgggcagtat ctggcagggg
    12001 ataaaggcgg cacgctggag atcgccaaac gctttgacag cggcgtggtg gtgggtggct
    12061 atgccacgat caccaacgtt ttgaaagagg agtacggcga aggggacttc accaaaggcg
    12121 tgtatgtctc ggtaccgttg gatctcttct cgtctggccc gacacgcagc cgtgcggcga
    12181 ttggctggac gccgctgacg cgtgacggtg gtcagcaact tgggcgtaag ttccagttgt
    12241 atgacatgac cagcgaccgt agcgtcaatt tccgctaagt catgggaaag gtgccagttt
    12301 tcgcactcac tggcaccttc attcttaatg gcctgtcgag ccatcaggaa cgcagttcac
    12361 ccccggtttg cgatctggtg tgtaaaggcg aaaacattct gtcttcctgt ccgtcgatat
    12421 cggttgcagg tctggttgcc tgagcgactg ggccatcatt gggctggaga cattgctttg
    12481 cggtaggtcg gctttatcag cagtaccgag cggaccagca taagcaggaa gaacagagac
    12541 tgataacatc aaagcagcaa aataaggctt catttttacc acctttatca ggttacgttt
    12601 catttgttcc agaggaacat tgtcgatttt tcgcgcattg ctggtggctg ggaatcacct
    12661 gaatgggtga tttttgaatt accggcttgg gtgcggtttg tcttgccgga tgcgccgcca
    12721 ggcgcggctt atccggccta cgggtaggta tatccggctt tggtggaggc gcgctccaaa
    12781 tccaggttga acaaaacata cacaaaaaat atagatctcc gtcacatttt tgcgttatac
    12841 aggaagctcg ccactgtgaa ggaggtactg ctatgacgtc actctctcgt ccgcgcgtgg
    12901 agtttatctc caccattttg cagaccgtac tcaatcttgg cctgctgtgc ctcggcctga
    12961 ttttggttgt cttcctcggc aaagaaacgg tgcatctggc agatgtgctg ttcgcgccag
    13021 aacaaaccag caaatatgag ctggtggttt actttctcta tttcgaattt atcgcgctga
    13081 ttgtgaagta ctttcagtcc ggttttcact tcccgctacg ttactttgtc tatattggga
    13141 tcaccgccat tgtgcgcttg atcatcgtcg atcataaatc gccactggat gtgttgatct
    13201 actcggctgc gatcctgtta ctggtgatca ctctgtggct gtgtaattcg aaacggctga
    13261 agcgggagta aaaagacagc acgccgaact ggcgcggcgt gctggacagg aagattacag
    13321 cgtagcagtt tgttgtgttt tcttcgtttc cggttcccag agggcttcca gctcctcaag
    13381 ggttttacct ttggtttccg gggcaaattt ccacataaat agtgctgcca gaacgcccat
    13441 acacccgtaa atccagtagg agaaaccgtt gtggaaatgg gccaccagcc aggagttttt
    13501 gtccatcatc gggaaggtcc aggagacgaa gtagttcgcc agccactggg ccgccaccgc
    13561 gattgccagc gccttaccac gaatagcatt cgggaagatt tccgacagca gtacccagca
    13621 taccggaccc caggacatgg caaaggcagc aacatagaac agcatcgaca gtagcgccac
    13681 aatacccggt gcctgagtgt aaaacgcggt accgaggcta aacataccga ttgccattcc
    13741 gagtgcgccg ataatttgca gtggcttacg accaaattta tccaccgtca taattgccag
    13801 aacggtgaag gtgaggttga taactccgac aataatggtc tgcaacagcg cgatatccgt
    13861 gctggccccc agcgttttga acacttccgg cgcgtagtac agcaccacat tgatgccgac
    13921 aaattgctgg aagatggaga gcataacgcc gatgacaatc acgcccacgc caaacatcag
    13981 cagacgacca ccggttttgc ggccatgatc cagggagtgt ttaatttcct gtactgcctg
    14041 agttgcaagc gtgttgccca taattttgcg caggatactt tccgcctgtt cttgcttgcc
    14101 gcgtgacatc agccagcgag ga
//
LOCUS       DAJVUJ010000107        14036 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.107, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000107 DAJVUJ010000000
VERSION     DAJVUJ010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14036
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.107"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            133..630
                     /locus_tag="OQQ48_002859"
     CDS             133..630
                     /locus_tag="OQQ48_002859"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1953560.1"
                     /translation="MKLRSLETTHTELGKLIPKITHIVRRETIIINPNDARADWLKTF
                     STLVPFVSGKKNAKTRQIEIKAEAKPSLNEVQEIIEKYSAEDREKRLWDNVGFATDKG
                     ITWVDKYRMRDILNVPSDDYSTYSAAYIYEQISHKRKEFISPILKELKALQSQPRIKK
                     ASGED"
     gene            631..1167
                     /locus_tag="OQQ48_002860"
     CDS             631..1167
                     /locus_tag="OQQ48_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1953561.1"
                     /translation="MLKAIAGIAKYTWIYMAIFIISLMIISSSDINYVYNDFANYCTL
                     LVSVSGMVFTIMGIWLAFLYPNALQRIVNPKTIETVDFTEQLEDTRRLEAIVGSVLKS
                     SFVMITLLVVYLVKLVFFKSHLYLESQDVIKNTSLALIIAISYMQIEAVISVILANVM
                     FINDLHNKREQRDIDDQV"
     gene            1118..2128
                     /locus_tag="OQQ48_002861"
     CDS             1118..2128
                     /locus_tag="OQQ48_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1953562.1"
                     /translation="MIYITSESKETLMIKYEGKMKKNIRVIPKNIHSKLRRLGNTVVA
                     GTSIAFTENQLKSGALEHLGIYFDNGVNAYETSVIPDPLQGKYSLKNVFGEEIVRKDL
                     PKETHYTEIESPNWGDSSNGTHTVRLPYEKYPRDIIPPTLIAIEINHKQPSDSHFIFN
                     FRVTRILDKQSNKFDDELLFDLNLLQENLGKCGIEDADKPISTYADTLIVSWEIFPPG
                     SKEETLARIFRGKNITSDKKNVAENRYDFFMSLEPKKIVTGNSTFSNYIGAMLEDDLV
                     VFENIEYGNAIYILYDNWDDISKLSRIDLLSGRAGSNFDRIIHSGNWKDEVRKKVAAG
                     RL"
     gene            complement(2183..3448)
                     /locus_tag="OQQ48_002862"
     CDS             complement(2183..3448)
                     /locus_tag="OQQ48_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCS1953563.1"
                     /translation="MKLNARQVETAKPKDKTYKMADGGGLYLEVSAKGSKYWRMKYRR
                     PSDKKEDRLAFGVWPTVTLAQARAKRDQAKKLLAQGIDPKAEQKEAQAENSGAYSFET
                     IAREWHASNKRWSEDHRSRVLRYLELYIFPHIGSSDIRQLKTSHLLAPIKKVDASGKH
                     DVAQRLQQRVTAIMRYAVQNDYIDSNPASDMAGALSTTKARHYPALPSSRFPEFLARL
                     AAYRGRVMTRIAVELSLLTFVRSSELRFARWDEFDFDKSLWRVPAKREEIKGVRYSYR
                     GMKMKEEHIVQLSRQAMILLNQLKQISGDKELLFPGDHDATKVMSENTVNSALRAMGY
                     DTKTEVCGHGFRTMARGALGESGLWSEDAIERQLSHSERNNVRAAYIHTSEHLDERRL
                     MVQWWADYLQANKGKTITPYDFAKINKSR"
     gene            complement(3612..3696)
                     /locus_tag="OQQ48_002863"
     tRNA            complement(3612..3696)
                     /locus_tag="OQQ48_002863"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3660..3662),aa:Leu,seq:caa)
     gene            3892..4911
                     /gene="ahr"
                     /locus_tag="OQQ48_002864"
     CDS             3892..4911
                     /gene="ahr"
                     /locus_tag="OQQ48_002864"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HCS1953564.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELRPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIKHVRDGKARYRVV
                     LKADF"
     gene            5041..6543
                     /locus_tag="OQQ48_002865"
     CDS             5041..6543
                     /locus_tag="OQQ48_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HCS1953565.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEKQGAAHFFGEPMLDIKDWMRTDTNGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKEV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDDVDRESAYEMLQKGFQASTKQQNNPPAKGKAVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRRR"
     gene            complement(6704..7786)
                     /gene="lptG"
                     /locus_tag="OQQ48_002866"
     CDS             complement(6704..7786)
                     /gene="lptG"
                     /locus_tag="OQQ48_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCS1953566.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQTFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(7786..8886)
                     /gene="lptF"
                     /locus_tag="OQQ48_002867"
     CDS             complement(7786..8886)
                     /gene="lptF"
                     /locus_tag="OQQ48_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCS1953567.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            9153..10664
                     /gene="pepA"
                     /locus_tag="OQQ48_002868"
     CDS             9153..10664
                     /gene="pepA"
                     /locus_tag="OQQ48_002868"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HCS1953568.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFEQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAILHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            10824..11267
                     /gene="holC"
                     /locus_tag="OQQ48_002869"
     CDS             10824..11267
                     /gene="holC"
                     /locus_tag="OQQ48_002869"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HCS1953569.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            11267..>14036
                     /gene="valS"
                     /locus_tag="OQQ48_002870"
     CDS             11267..>14036
                     /gene="valS"
                     /locus_tag="OQQ48_002870"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCS1953570.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAV"
BASE COUNT         3679 a         3416 c         3631 g         3310 t
ORIGIN      
        1 gactatgtac actcatctat tactaataga gttaaacata gcaaaagggt taaaaataaa
       61 accgagaaag gttatataag gctatctaat accgatgacg aagacttata taaatatatg
      121 tacaggtttg atatgaagct cagaagctta gaaaccactc atactgagtt aggtaaacta
      181 ataccaaaaa taacacacat agttcgcagg gaaactatta ttataaatcc aaatgatgca
      241 cgagctgatt ggttaaagac ctttagcact ctcgttccat ttgtgtccgg aaagaaaaat
      301 gccaaaacaa gacaaattga aataaaagca gaagcaaaac catccttgaa tgaagttcaa
      361 gaaatcattg aaaagtattc ggctgaggat agagaaaaac gtctttggga caatgttgga
      421 tttgctacag acaagggtat aacttgggtg gataaatata gaatgcgtga tattctcaat
      481 gtcccttctg acgactattc aacatattcc gccgcatata tttacgaaca aatatcacac
      541 aaaagaaaag aatttatttc ccctatatta aaagaattaa aagcattaca atctcaacca
      601 agaataaaga aagcatctgg agaggattga atattgaaag ctatagcagg catagccaaa
      661 tacacatgga tatacatggc gatattcatt atatcgctga tgattatttc gtcatccgat
      721 attaactatg tgtataacga ttttgccaac tattgcactc ttctcgtatc agtttctgga
      781 atggttttta ctattatggg aatttggtta gcgtttcttt acccaaatgc tttgcagcgt
      841 attgtgaacc ccaaaactat agaaaccgtt gatttcaccg aacaactgga agatacaaga
      901 agattagaag caatagttgg aagtgttcta aaatcatctt tcgttatgat tactttactt
      961 gttgtatact tagttaaact agtgtttttc aaaagtcatc tttatttaga gagccaagat
     1021 gtaataaaaa acacatcttt agcattaatt attgctattt cgtacatgca gattgaggca
     1081 gtaattagcg taatacttgc aaatgttatg tttatcaatg atttacataa caagcgagag
     1141 caaagagaca ttgatgatca agtatgaggg aaaaatgaaa aagaatatac gtgttatacc
     1201 taaaaatatt catagcaagt taagaaggct tggtaataca gttgttgctg gcacttctat
     1261 tgcatttacg gaaaaccaat taaaaagtgg agcattagag caccttggaa tatattttga
     1321 taatggtgtt aatgcttatg agacgtcggt cataccagat ccattgcagg gtaaatattc
     1381 acttaaaaat gtttttggtg aagaaattgt cagaaaagac ctcccaaaag aaactcatta
     1441 cactgaaatt gagtcaccta actggggtga tagctctaat ggtacacata ctgttagatt
     1501 accttacgag aaatatccca gagacattat accaccaaca ctcatagcca ttgaaattaa
     1561 tcacaaacag ccctcggaca gccatttcat atttaacttc agagtaacta gaattttaga
     1621 caaacaatca aataagtttg atgatgaatt attatttgat ttaaatcttc ttcaagaaaa
     1681 tcttggtaaa tgtggaattg aggatgctga taaacccata tcgacttatg ctgatacact
     1741 tatagtctca tgggaaatat ttccaccagg tagtaaagaa gaaactttag caagaatttt
     1801 tagaggtaaa aatatcactt ccgacaagaa aaatgtcgct gaaaatagat atgacttctt
     1861 catgagtctt gaacctaaaa aaatcgtcac aggaaacagc acgttcagta attatattgg
     1921 agctatgctg gaggacgatt tggttgtatt tgaaaacata gaatatggta atgctatata
     1981 tatcctctat gataactggg atgatatcag caagctatct cgaattgact tattatcagg
     2041 aagagcagga agtaatttcg atagaataat ccatagtggc aattggaaag atgaagtacg
     2101 aaaaaaagtt gctgctggaa gattatgatc aaagtgttta atacagaaat aaacgcctat
     2161 atattatagg cgtttatttt cactatcgac ttttgtttat tttagcaaaa tcataaggtg
     2221 ttattgtttt gcccttattt gcttggagat aatcagccca ccattgaacc atcaatcgac
     2281 gttcatccaa atgttcagag gtgtgaatat aagcagcacg tacattatta cgctctgaat
     2341 ggctcaactg tcgttctata gcatcttcac tccataatcc ggactcgccc aacgcaccac
     2401 gggccatcgt tctaaacccg tgcccgcaaa cttcggtttt cgtgtcatag cccatcgcac
     2461 gcaatgcgct atttaccgtg ttttcgctca taaccttagt tgcgtcatga tcacccggaa
     2521 aaagcagctc tttatctcca ctaatctgct ttaactggtt tagcaaaatc atcgcctgcc
     2581 gactaagctg aacgatatgc tcctctttca tcttcatgcc acggtacgag taacgcacac
     2641 ctttaatttc ttctcgtttt gcaggtactc gccagagaga tttatcgaag tcgaactcat
     2701 cccaacgcgc gaaacgtaac tcactggaac gcacaaaagt tagtaaggaa agctcaaccg
     2761 cgatccgcgt cattacacgg ccacgatatg cagcaagacg agcaagaaac tcagggaacc
     2821 ggctggaggg caaagcgggg taatgtcgcg ctttggttgt cgatagcgca ccggccatat
     2881 cactggctgg atttgagtcg atgtaatcgt tctgtactgc ataacgcata atagccgtga
     2941 cacgctgctg aaggcgctgt gcaacgtcat gcttaccact ggcatcaact tttttaatcg
     3001 gggctaatag gtggctagtt ttgagctgac ggatgtcaga tgaaccaata tgaggaaaga
     3061 tataaagctc aagataacgt agaacgcgtg atcggtgatc ttcactccag cgcttattac
     3121 tagcatgcca ttcacgagcg atagtttcga aagaatatgc ccccgaattc tcggcctgag
     3181 cttctttctg ttcggctttt ggatcaatgc cctgcgctaa cagcttttta gcttgatcgc
     3241 gctttgctct tgcctgagca agcgtcacag taggccaaac accaaacgcg agacgatcct
     3301 cttttttgtc agagggacgt ctgtatttca tgcgccagta tttggaacct ttggccgaaa
     3361 cctcaagata caaaccacca ccatcggcca ttttgtaggt tttgtctttt ggctttgcgg
     3421 tctcgacctg tctggcgttg agcttcattt gggggcacat ttctaatcga agttaagatg
     3481 cccccaatta tgcccccaat gatatccgga tttcaacgga caacctcgga agacgcagga
     3541 cgaaaaaatc gctgcaagca ttgattttaa agggatattt ggactttctc ggatggtctt
     3601 ggaagtaaga atggtgccga aggccggact cgaaccggca cgtatttcta cggttgattt
     3661 tgaatcaact gcgtctaccg atttcgccac ttcggcactg aagaggtatg cggaaaacgt
     3721 tgtggattat acctgtcgca cgccaccatg caagcgccag cttgctcgca cctcgctaag
     3781 tgctgaaatt ttcagcattg cacacagcga tgtgtaacct ttgtcacact ccaggcaccc
     3841 cgccctgcca tgctctacac ttcccaaaca acaccagaga aggaccaaaa aatgtcgatg
     3901 ataaaaagct atgccgcaaa agaagcgggc ggcgaactgg aagtttatga gtacgatccc
     3961 ggtgagctga ggccacaaga tgttgaagtg caggtggatt attgcgggat ctgccattcc
     4021 gatctatcga tgatcgacaa tgaatgggga ttttcacaat atccgctggt tgccgggcat
     4081 gaggtgattg ggcgcgtggt ggcactcggg agcgccgcgc aggataaagg tttgcaggtc
     4141 ggtcagcgtg tcgggattgg ctggacggcg cgtagctgtg gtcactgcga cgcctgtatt
     4201 agcggtaatc agatcaactg cgagcaaggt gcggtgccga cgattatgaa tcgcggtggc
     4261 tttgccgaga agttgcgtgc ggactggcaa tgggtgattc cactgccaga aaatattgat
     4321 atcgagtccg ccgggccgct gttgtgcggc ggtatcacgg tctttaaacc actgttgatg
     4381 caccatatca ctgctaccag ccgcgttggg gtaattggta ttggcgggct ggggcatatc
     4441 gctataaaac ttctgcacgc aatgggatgc gaggtgacgg cctttagttc taatccggcg
     4501 aaagagcagg aagtactggc gatgggtgcc gataaagtgg tgaatagccg cgatccgcag
     4561 gcactgaaag ccctgtcggg gcagtttgat ctcattatca atactgtgaa cgtcagcctc
     4621 gactggcagc cttattttga ggcgctgacc tatggcggta atttccatac ggtcggtgcg
     4681 gttctcacgc cgctgtctgt tccggccttt acgttaattg cgggcgaccg cagcatctct
     4741 ggttctgcta ccggcacgcc ttatgagctg cgaaagctga tgcgctttgc cgcccgtagc
     4801 aaggttgcgc cgaccaccga actgttcccg atgtcgaaaa ttaacgatgc catcaagcat
     4861 gtgcgcgacg gtaaggcgcg ttaccgcgtg gttctgaaag ccgacttctg atccctttgc
     4921 cgggtgcctg tcgcccggca cttaaatctc tttggaaggc gtctcgcaat ctgctgaatt
     4981 tagcggtgaa gagtgcgcag ccgttgccta tactccaggc aggaaactgg aggaaatctc
     5041 atgagcgaac ccctattaat tgcccgcacg ccggacacag aacttttttt actgccggga
     5101 atggctaacc gtcacgggct gattactggc gcaacgggga cgggtaaaac cgttacgctg
     5161 caaaaactgg cagagtcatt gtcggaaatc ggcgtgccgg tgtttatggc tgatgtgaaa
     5221 ggcgatctga ccggtatcgc gcaggcagga acggcgtcgg aaaaactgct cacaaggctt
     5281 aaaaatatcg gcgtcaatga ctggcaaccg catgctaatc cggtggtggt gtgggatatc
     5341 tttggcgaga aaggccatcc ggtgcgggcg acggtttcag acctggggcc gctgttgctg
     5401 gcgcggctgt tgaatctcaa cgatgtgcaa tctggcgtgc tgaatatcat cttccgtatt
     5461 gctgacgatc agggattgtt gctgctcgac tttaaagatc tgcgggcgat tacccagtac
     5521 atcggcgata acgccaaatc tttccagaat cagtacggta atatcagtag cgcatcggtt
     5581 ggtgccatcc agcgcggttt actgtcgctg gaaaagcaag gtgcggcgca tttctttggc
     5641 gagccgatgc tggatatcaa agactggatg cgcaccgata ccaacggtaa aggcgttatc
     5701 aatatcctca gcgccgagaa gctttatcag atgccgaaac tgtacgccgc cagcctgctg
     5761 tggatgcttt cagagttgta tgaacaattg ccggaagcgg gcgatctgga gaaaccaaaa
     5821 ctggtgtttt tcttcgacga ggcacatctg ctgtttaacg atgcaccgca ggtactgctg
     5881 gataagattg agcaggtaat aaggcttatt cgctcaaaag gcgtgggcgt ctggttcgtt
     5941 tcgcaaaacc cgtctgatat tccggataat gtgctcgggc agctcggtaa tcgcgttcaa
     6001 cacgctttgc gggcttttac gcccaaagat cagaaagagg taaaagctgc ggcgcaaacc
     6061 atgcgggcca atccggcgtt tgataccgaa aaggcaatcc aggaactggg gaccggcgag
     6121 gcgttaatct cgtttctcga tgcaaaagga agtccttctg tggtggaacg ggcgatggtg
     6181 attgcgcctt gttcgcggat gggaccggtg acggaagatg agcgcaatgg cttgattaac
     6241 cactccccgg tgtatggcaa gtacgaggat gacgtggacc gggaatccgc ctatgagatg
     6301 ttgcaaaaag gctttcaggc cagtaccaag cagcaaaata atcctcccgc gaaagggaaa
     6361 gcggtggcgg tggatgacgg tattcttggt ggattgaagg atattttgtt tggcactacc
     6421 ggaccacgcg gcgggaagaa agatggtgtg gtgcaaacaa tggcgaaaag cgccgcccgc
     6481 caggtgacga atcagattgt acgtgggatg ttggggagtt tgctgggggg aagaagaagg
     6541 taagtcaggc gagatttgtc tttgcctgat gcgacgctgg cgcgtcttat catgcctaca
     6601 ccactgcaat atattggatt ttcatgattt ttcaggcatg aacgaagcac actttgctaa
     6661 caataataaa gggagctttc gctcccttta ttcgttcatt cggttacgat tttctcatta
     6721 acagccacag gctgattaag aagaagctgg cgcttggcaa cagtgcgccg atgatcggcg
     6781 ggatgccata aaccaacgtc agcgggccga agatctggtc cagtacgtag aagacaaaac
     6841 cgaagctgat accggtgacc acacgcacgc ccatcggtac gctacgcagt gggccaaaga
     6901 tgaacgacag cgccatcagc atcatcaccg ccacggatag cggctggaag attttgctcc
     6961 acatattgag ctgataacgt ccggcatcct gaccgctcga cttcagatac ttcacgtagt
     7021 tgtgcaaacc gctgattgag agtgcatccg ggtccagcgc caccacgccc agtttgtccg
     7081 gcgtgaggtt ggttttccag gtgccgctca ccgtctgcga accggtgatc tgtttcggat
     7141 tggtcagatc agattcatca acctgcgaca gacgccagac tttatgttcc gggtcaaact
     7201 tcgcagtagc ggcatagcgt acggattgca gacgacgatt ctcgttaaag gcataaatac
     7261 tgatgccacc taactcttcg tcacctttaa cccgctcaat gtaaacgaag ttgttgccat
     7321 ctttcgccca taagccttgc tgggtagaga gcaacgagcc gccgtacatc gcctgcgcac
     7381 ggtagttacg cgccatctgc tcgccctgcg gcgcgaccca ttcaccaatc gccatcgtca
     7441 gcaagaccag cggaatggcg gttttcatca ccgacagcgc aacctgcata cgggtgaaac
     7501 cagaagcctg catcaccacc agttcgctgc gctgcgccag catcccaaga ccaagcaacg
     7561 ccccaagcag agccgccatc gggaagaatg tttgcacatc tttcggcacg ctcagcaagg
     7621 tatacattcc tgcgcctaac gcgtcgtaac tcccctgccc ggcttttttc agctgatcga
     7681 caaatttgat aatgcccgac agcgacacca gcatgaacag cgtcatcatg atggtggtga
     7741 aaatagtttt accgatatag cggtcaagta cgccaaaagg ttgcatcaca ccgctccttt
     7801 acgcgaaaaa ctggcgcgca ggcggcggac cggcacggtg tcccaaaggt tgagaacaat
     7861 cgccaaagcc agataaatca ggttaacggt ccacatccac agcgtcgggt ccagcttacc
     7921 tttaccgccg ttcgatttca gggaggtctg gatcaggaag aaaagtagat acagcagcat
     7981 ggctggcagc atcgatagta cgcgtccctg acgcgggtta accacgctca gcggtacgac
     8041 cataagtgcc atcataaaca cggtgaatac caacgtgata cgccagttca gttctgcgcg
     8101 ggcacgatcg gtgtcagtgt tccacaatgt acgcatgtcc atctggtcgg tatcgttcgg
     8161 gtcgagcgct accgcctggt gaccaatgat cgcctgataa tcctggaagt ccgtaatgcg
     8221 gaaatcacgt aacagtgcag tgccttcgaa gcgcgttccc tggttgagag tgacgacctg
     8281 ggagccgtcg cgcagctggg ttaaatgtcc ggaatcggcc accaccacag aaggacgtgc
     8341 attacctttt ggtcgaattt gcgcgaggaa cacatctttg aaatcgctgc cgtcaacgct
     8401 ttcgatgaac agcaccgagc tgccattagt cgcttgctgg aattgccctt gcgccagcgc
     8461 cgccatgcca gggttcgctt tcgcttctgc taacacttca tcctgatgac gcgatgacca
     8521 cggtcccgcc cacatcacgt taaccgccgc gacgattgca gtgaataccg caaggatcat
     8581 tgccgctttc accagaaccg ctttgctcag gccgcaggca tgcattaccg taatttcact
     8641 ttcggtatac agtttgccca gcgtcatcag cagcccgagg aacaggctta atggcaggat
     8701 aagctgcgcc atttccggca cgcccaaccc gagaagggag agcaccagat tcgccggaat
     8761 atcgccgtca accgctgcgc cgaggatcct cactaacttt tgacagaaga agatcaaaag
     8821 caagatgaag agtatcgcca gctggctttt gagcgtctcc cgcaccagat atcttatgat
     8881 tatcacttta aatacgcccg taaaaactcg tcttttgcag gattttagct tgtttcatgg
     8941 cttaaacgtc atttattctc ttgagtcgtc gaaatcgtcg ctaagataat tatactcaac
     9001 ggattcacct ctcagatttt gttctgacgt gccaatgccg taataacgtt aagattaaca
     9061 cgaagtcatc gcaacagcgg acatgagtta cgaaagcttg caattctatc tgtagccacc
     9121 gccgttgtct ttaagattca ggagcgtagt gcatggagtt tagtgtaaaa agcggtagcc
     9181 cggagaaaca gcggagtgcc tgcatcgtcg tgggcgtctt cgaaccacgt cgcctttctc
     9241 cgattgcaga acagctcgat aaaatcagcg atgggtacat cagcgccctg ctacgtcggg
     9301 gcgaactgga aggaaaaccg gggcagacat tgttgctgca ccatgttccg aatgtacttt
     9361 ccgagcgaat tctccttatt ggttgcggca aagaacgtga gctggatgag cgtcagtaca
     9421 agcaggttat tcagaaaacc attaatacgc tgaatgatac tggctcaatg gaagcggtct
     9481 gctttctgac tgaactgcac gttaaaggcc gtaacaacta ctggaaagtg cgtcaggctg
     9541 tcgagacggc aaaagagacg ctctacagtt tcgaacagct gaaaacgaac aagagcgaac
     9601 cgcgtcgtcc gctgcgtaaa atggtgttca acgtgccgac ccgccgtgaa ctgaccagcg
     9661 gtgagcgcgc gatcctgcac ggtctggcga ttgccgccgg gattaaagca gcaaaagatc
     9721 tcggcaatat gccgccgaat atctgtaacg ccgcttacct cgcttcacaa gcgcgccagc
     9781 tggctgacag ctacagcaag aatgtcatca cccgcgttat cggcgaacag cagatgaaag
     9841 agctggggat gcattcctat ctggcggtcg gtcagggttc gcaaaacgaa tcgctgatgt
     9901 cggtgattga gtacaaaggc aacgcgtcgg aagatgcacg cccaatcgtg ctggtgggta
     9961 aaggtttaac cttcgactcc ggcggtatct cgatcaagcc ttcagaaggc atggatgaga
    10021 tgaagtacga tatgtgcggt gcggcagcgg tttacggcgt gatgcggatg gtcgcggagc
    10081 tacaactgcc gattaacgtt atcggcgtgt tggcaggctg cgaaaacatg cctggcggac
    10141 gagcctatcg tccgggcgat gtgttaacca ccatgtccgg tcaaactgtt gaagtgctga
    10201 ataccgacgc tgaaggccgc ctggtactgt gcgacgtgtt aacttacgtt gagcgttttg
    10261 agccggaagc ggtgattgac gtggcgacgc tgaccggtgc ctgcgtgatc gcgctgggtc
    10321 atcacattac cggtctgatg gcgaaccata atccgctggc ccatgaactg attgccgcgt
    10381 ctgaacaatc cggtgaccgc gcatggcgct taccgctggg tgacgagtat caggaacagc
    10441 tggagtccaa ttttgccgat atggcgaaca ttggcggtcg tcctggtggg gcgattaccg
    10501 caggttgctt cctgtcgcgc tttacccgta agtacaactg ggcgcacctg gatatcgccg
    10561 gaaccgcctg gcgttctggt aaagcaaaag gcgccaccgg tcgtccggtg gcgttgctgg
    10621 cacagttcct gttaaaccgc gctgggttta acggcgaaga gtaattgcgt caggcaaggc
    10681 tgttattgcc ggatgcggcg tgaacgcctt atccgaccta cacagcactg aactcgtagg
    10741 cctgataaga cacaacagcg tcgcatcagg cgctgcggtg tatacctgat gcgtatttaa
    10801 atccaccaca agaagcccca tatatgaaaa acgcgacgtt ctaccttctg gacaatgaca
    10861 ccaccgtcga tggcttaagc gccgttgagc aactggtgtg tgaaattgcc gcagaacgtt
    10921 ggcgcagcgg taagcgcgtg ctcatcgcct gtgaagatga aaagcaggct taccggctgg
    10981 atgaagccct gtgggcgcgt ccggcagaaa gctttgttcc gcataattta gcgggagaag
    11041 gaccgcgcgg cggtgcgccg gtggagatcg cctggccgca aaaacgcagc agcagcccgc
    11101 gcgatatatt gattagtctg cgaacaagct ttgcagattt tgccaccgct ttcacagaag
    11161 tggtagactt cgttccttat gaagattctc tgaaacaact ggcgcgcgaa cgctataaag
    11221 cctaccgcgt ggctggtttc aacctgaata cggcaacctg gaaataatgg aaaagacata
    11281 taacccacaa gatatcgaac agccgcttta cgagcactgg gaaaagcagg gctactttaa
    11341 gcctaatggc gatgaaagcc aggaaagttt ctgcatcatg atcccgccgc cgaacgtcac
    11401 cggcagtttg catatgggtc acgccttcca gcaaaccatc atggatacca tgatccgcta
    11461 tcagcgcatg cagggcaaaa acaccctgtg gcaggtcggt actgaccacg ccgggatcgc
    11521 tacccagatg gtcgttgagc gcaagattgc cgcagaagaa ggtaaaaccc gtcacgacta
    11581 cggccgcgaa gctttcatcg acaaaatttg ggaatggaaa gcggaatccg gcggcacgat
    11641 tacccgtcag atgcgccgtc tcggcaactc cgtcgactgg gagcgtgaac gcttcacgat
    11701 ggacgaaggc ctgtccaatg cggtgaaaga agttttcgtt cgtctgtata aagaagacct
    11761 gatttaccgt ggcaaacgcc tggtaaactg ggatccgaaa ctgcgcaccg ctatctctga
    11821 cctggaagtg gaaaaccgcg aatcgaaagg ttcgatgtgg cacatccgct atccgctggc
    11881 tgacggcgcg aaaaccgcag acggtaaaga ttatctggtg gttgcgacta cccgtccgga
    11941 aaccctgttg ggcgataccg gcgtggccgt taacccggaa gatccgcgtt acaaagacct
    12001 gattggcaaa tatgtcattc taccgctggt taaccgtcgt attccgatcg ttggcgacga
    12061 acacgccgac atggaaaaag gcaccggctg cgtgaaaatc actccggcgc acgactttaa
    12121 cgactatgaa gtgggtaaac gtcacgcgct gccgatgatc aacatcctga cctttgacgg
    12181 cgatatccgt gaaagcgccc aggtgttcga taccaaaggt aacgaatctg acgtttattc
    12241 cagcgaaatc ccggcagagt tccagaaact ggagcgtttt gctgcacgta aagcagtcgt
    12301 tgctgcggtt gacgcgctcg gcctgctgga agaaattaaa ccgcacgacc tgaccgtgcc
    12361 ttacggcgac cgtggcggcg tagttatcga accgatgctc accgaccagt ggtacgtgcg
    12421 tgccgatgta ctggcgaaac cggcggttga ggcggttgag aacggcgaca ttcagttcgt
    12481 accgaagcag tacgaaaaca tgtacttctc ctggatgcgc gatattcagg actggtgtat
    12541 ctctcgtcag ctgtggtggg gtcaccgtat ccctgcatgg tacgacgaag caggtaacgt
    12601 ttatgttggc cgcaacgaag aagaagtgcg caaggaaaat aacctcggtg ccgacgttgc
    12661 cctgcgtcag gacgaagacg ttctcgacac ctggttctct tctgcgctgt ggaccttctc
    12721 taccctaggc tggccggaaa acaccgacgc actgcgccag ttccacccaa ccagcgtgat
    12781 ggtatctggt ttcgacatca ttttcttctg gattgcccgc atgatcatga tgaccatgca
    12841 cttcatcaaa gatgaaaatg gcaaaccgca ggtgccgttc cacaccgttt acatgaccgg
    12901 cctgattcgt gacgacgaag gccagaagat gtccaaatcc aagggtaacg ttattgaccc
    12961 gctggatatg gttgacggta tttcgctgcc ggaactgctg gagaaacgta ccggcaatat
    13021 gatgcagccg cagctggcgg acaaaatccg taagcgcacc gagaaacagt tcccgaacgg
    13081 cattgagccg cacggcaccg atgccctgcg cttcaccctg gcggcgctgg cttctaccgg
    13141 tcgtgacatc aactgggata tgaaacgcct ggaaggctac cgtaacttct gtaacaagct
    13201 gtggaacgcc agccgctttg tgctgatgaa cacggaaggt caggattgcg gcttcaacgg
    13261 tggtgaaatg acgctgtcgc tggcggaccg ctggattctg gcagagttca accagaccat
    13321 caaagcgtac cgcgaagcgc tggacagctt ccgcttcgat atcgctgcag gcattctgta
    13381 tgagttcacc tggaaccagt tctgcgactg gtatctggag ctgactaagc cggtaatgaa
    13441 cggtggaacc gaagccgaac tgcgcggtac tcgccatacg ctggtgactg tactggaagg
    13501 tctgctgcgc ctcgcgcatc cgatcattcc gttcatcacc gaaaccatct ggcagcgtgt
    13561 gaaagtactt tgcggtatca ctgccgacac catcatgctg cagccgttcc cgcagtacga
    13621 tgcatctcag gttgatgaag ccgcactggc tgacaccgag tggctgaaac aggcgatcgt
    13681 tgcggtacgt aacatccgtg cagaaatgaa catcgcgccg ggcaaaccgc tggagctgct
    13741 gctgcgtggt tgcagcgcgg atgcagaacg tcgcgtaaat gaaaaccgtg gcttcctgca
    13801 aaccctggcg cgtctggaaa gcatcaccgt gctgcctgcc gatgacaaag gtccggtttc
    13861 cgttacgaag atcatcgacg gcgcagagct gctgatcccg atggctggcc tcatcaacaa
    13921 agaagatgag ctggcgcgtc tggcgaaaga agtggcgaag atcgaaggcg aaatcagccg
    13981 tatcgagaac aagctggcga atgaaggctt tgtcgcccgc gcaccggaag cggtca
//
LOCUS       DAJVUJ010000108        13889 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.108, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000108 DAJVUJ010000000
VERSION     DAJVUJ010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13889
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.108"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            220..1167
                     /locus_tag="OQQ48_002871"
     CDS             220..1167
                     /locus_tag="OQQ48_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001361319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCS1953571.1"
                     /translation="MKNVLVTVPSFSARCVSASKLLRENNFNLIIKNNVEHLLKSEST
                     ALRESICAVIAGKDCYQADTLSLLPGVKIISRFGTGIDNIDLRAAQQSGIVVNNAVGI
                     NSNAVAEFIIGLIFASMRNIPGSYHAMQNGYWGESHGYELQGKRIGMVGYGNIGKTLA
                     KRLSGFDVELLAYDKQPDYQVADKVGVQFVSIEDIFMQSHVIIVLLPFSPELENFISH
                     KYLSMMRNGALIINAARGKLLDEGALLQVIEERNVFAALDVFSSEPLAQFSPLLHAKN
                     IITTPHIAAATVESYQQTGTHVAQSIIDYFAGREIKNVL"
     gene            complement(1246..1322)
                     /locus_tag="OQQ48_002872"
     tRNA            complement(1246..1322)
                     /locus_tag="OQQ48_002872"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1286..1288),aa:Met,seq:cat)
     gene            1531..2628
                     /gene="mltA"
                     /locus_tag="OQQ48_002873"
     CDS             1531..2628
                     /gene="mltA"
                     /locus_tag="OQQ48_002873"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HCS1953572.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKSTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            2705..3511
                     /gene="tcdA"
                     /locus_tag="OQQ48_002874"
     CDS             2705..3511
                     /gene="tcdA"
                     /locus_tag="OQQ48_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HCS1953573.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSIGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(3562..4005)
                     /gene="csdE"
                     /locus_tag="OQQ48_002875"
     CDS             complement(3562..4005)
                     /gene="csdE"
                     /locus_tag="OQQ48_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HCS1953574.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLTQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVLGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRGQLSASRSQGLNALSEAIIAAAKQV"
     gene            complement(4005..5210)
                     /gene="csdA"
                     /locus_tag="OQQ48_002876"
     CDS             complement(4005..5210)
                     /gene="csdA"
                     /locus_tag="OQQ48_002876"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HCS1953575.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALSQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAENWSHSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            5402..5629
                     /locus_tag="OQQ48_002877"
     CDS             5402..5629
                     /locus_tag="OQQ48_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HCS1953576.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            5980..6897
                     /gene="gcvA"
                     /locus_tag="OQQ48_002878"
     CDS             5980..6897
                     /gene="gcvA"
                     /locus_tag="OQQ48_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HCS1953577.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            6916..7311
                     /locus_tag="OQQ48_002879"
     CDS             6916..7311
                     /locus_tag="OQQ48_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HCS1953578.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            7304..8404
                     /gene="rlmM"
                     /locus_tag="OQQ48_002880"
     CDS             7304..8404
                     /gene="rlmM"
                     /locus_tag="OQQ48_002880"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HCS1953579.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENSGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(8448..9179)
                     /gene="fucR"
                     /locus_tag="OQQ48_002881"
     CDS             complement(8448..9179)
                     /gene="fucR"
                     /locus_tag="OQQ48_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HCS1953580.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICYELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(9237..9659)
                     /gene="fucU"
                     /locus_tag="OQQ48_002882"
     CDS             complement(9237..9659)
                     /gene="fucU"
                     /locus_tag="OQQ48_002882"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HCS1953581.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(9661..11079)
                     /gene="fucK"
                     /locus_tag="OQQ48_002883"
     CDS             complement(9661..11079)
                     /gene="fucK"
                     /locus_tag="OQQ48_002883"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HCS1953582.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIEQLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERTHAWFFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGADQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     SADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            complement(11076..12821)
                     /gene="fucI"
                     /locus_tag="OQQ48_002884"
     CDS             complement(11076..12821)
                     /gene="fucI"
                     /locus_tag="OQQ48_002884"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HCS1953583.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEVLQERL"
     gene            complement(12854..>13889)
                     /gene="fucP"
                     /locus_tag="OQQ48_002885"
     CDS             complement(12854..>13889)
                     /gene="fucP"
                     /locus_tag="OQQ48_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HCS1953584.1"
                     /translation="ITGLFLYALGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAA
                     NPFVTVLGPESSGHFRLNLAQTFNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQ
                     LSAYKHSLVLSVQTPYMIIVAIVLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRL
                     ARIRHWRWAVLAQFCYVGAQTACWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGR
                     FTGTWLISRFAPHKVLAAYALIAMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFS
                     LGIKNLGQDTKYGSSFIVMTIIGGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIF
                     ARFRSQTATN"
BASE COUNT         3363 a         3407 c         3652 g         3467 t
ORIGIN      
        1 aggtttagca ccgtcatgct cgtctactgt tgttctggtt gttggtgatg cggtggcgct
       61 tgcactctct gaattgaaaa aatttactcg tgctgatttc ggcttatatc atccaggagg
      121 tgcactcggc attaaagcaa attcataatt tcaaagtata aatacagtga ccggtcacgc
      181 catcttttgg ggtggcgttc attgaagtga gtgtcagcta tgaaaaatgt tctggtcacc
      241 gtccccagct tttcagcacg ctgtgtttcc gccagcaagt tgctaagaga gaataatttt
      301 aatcttatta ttaagaataa tgtggagcat ctgcttaaat cagaatccac tgcattacga
      361 gaatctattt gcgcggtcat cgccggaaaa gattgttatc aggcggacac gctatcattg
      421 ctgcctggtg tgaaaataat atcgagattc gggacgggga tagataatat tgatcttcgt
      481 gcagcacaac aatccggtat tgtagtcaat aatgcggttg ggattaattc aaatgctgtc
      541 gcggaattta tcattggact tatcttcgcg agcatgagaa atattcccgg cagctatcat
      601 gcgatgcaaa atggctactg gggtgagtcg catggctatg aattacaagg caaacggatt
      661 ggcatggttg gctacggcaa tataggtaaa accctggcaa aaaggttatc cggttttgat
      721 gttgagcttt tagcttatga caaacaaccc gattatcagg ttgctgacaa agttggagtt
      781 cagtttgtat caattgaaga tatctttatg cagtcgcatg tcatcattgt tcttttgcct
      841 ttttctcctg agctggagaa cttcattagt cataaatatt tgtcgatgat gcgcaatggt
      901 gctctaatca tcaatgccgc aaggggaaag ttgctggatg agggcgcttt actacaggtg
      961 attgaagaac gaaatgtgtt tgcggcgctg gatgtgttta gctctgaacc gttggcgcaa
     1021 tttagcccac tgctacatgc caaaaatatc attacaacgc cgcatattgc ggccgccacc
     1081 gttgagtcat accagcaaac cggtacacac gttgcccagt cgataattga ttacttcgca
     1141 ggaagggaga taaaaaacgt gttgtgagtt cagcgaacga agtttttaaa tacaaaaaaa
     1201 ccgcaatttg tggaacacat tgcggtttat cagcattcta agtattggtt gcgggggccg
     1261 gatttgaacc gacgaccttc gggttatgag cccgacgagc taccaggctg ctccaccccg
     1321 cgtccgtgga tgcgcactat actccgctcg cgttttcatg caaccttttt tttaccccga
     1381 tcacggattt agcatgattt tgaacaaaaa caaaccacta aagcctattt tttgtgcaaa
     1441 atttgcgtca gggagaggct gctcatttca ttaaacgcgg cggtttgtta tcttcgttgc
     1501 gccttatttt ttaacctgaa gaagagaaca atgaaaggac gttgggtaaa gtaccttctt
     1561 atgggcacgg ttgtggcaat gcttgccgcc tgctcttcca aatcaaccga tcgcggacag
     1621 caatataaag acgggaaatt tacccagcct ttctctctgg tgaaccagcc agatgccgtt
     1681 ggcgcgccga ttaacgccgg tgattttgcc gagcaaatta accatatccg taattcgtca
     1741 ccgcgtctgt atggcaacca gagtaatgtt tataacgcgg tgcaagagtg gctgcgcgca
     1801 ggcggtgata cccgcaatat gcgccagttc ggcattgatg cctggcagat ggaaggtgcc
     1861 gacaactatg gtaacgtgca gtttacaggc tattacacgc cggtaattca ggcgcgccat
     1921 acccgccagg gcgagttcca gtatcctatt taccgtatgc cgccaaaacg tggtcgtctg
     1981 ccgtctcgtg cggagatcta cgcgggggca ttgggcgata aatacatcct cgcttacagc
     2041 aactctctga tggataactt cattatggat gtgcagggta gtgggtatat cgactttggt
     2101 gatggcagtc cgcttaactt tttcagctat gcagggaaaa acggtcatgc ctatcgcagc
     2161 attggtaagg tgctgatcga ccgtggcgaa gtgaaaaaag aagatatgtc gatgcaggcg
     2221 attcgtcatt ggggcgaaac acacagtgaa gccgaggttc gcgagctgct ggaacagaac
     2281 ccgtctttcg tcttctttaa accgcaatct tttgcaccgg tgaaaggggc aagtgcggtg
     2341 ccgctggttg gccgtgcttc tgttgcctcc gatcgttcaa ttattccgcc aggtactacc
     2401 ttgctggcag aagtgccgct gctggataat aacggcaaat ttaacggtca gtacgaattg
     2461 cgtctgatgg tggcgctgga tgtcggtggc gcaatcaaag gccaacactt cgatatctat
     2521 caagggatcg ggccggaagc cggacatcgc gcaggttggt acaaccacta tggacgtgtc
     2581 tgggtgctga aaaccgcccc gggcgcaggt aacgtcttta gcggctgatg tgttattctg
     2641 tataccgttt ttacggatac gacagggtga gggataacct cacccttttt aattctgagg
     2701 tgctatgtct gtggtaatta gtgatgcatg gcgtcagcgt tttggtggca cagcgcgtct
     2761 gtatggtgaa aaagcgttgc aactgtttgc tgacgcgcat atttgtgtgg ttggtatcgg
     2821 tggtgtcggt tcctgggcgg cggaagcgct ggcgcgcacg gggattggcg caatcacgct
     2881 tatcgatatg gatgatgtgt gcgtcaccaa taccaatcgg caaattcacg ctctgcgcga
     2941 taacgtcggg ctggcaaaag cggaagtcat ggcggagcgc attcgccaga ttaacccgga
     3001 gtgccgtgta acggtggtgg atgatttcgt gacgccggat aacgtagcgc aatatatgag
     3061 tgccggttat tcgtacgtga ttgatgccat tgatagtgta cgacccaaag cggcgctgat
     3121 tgcttattgt cggcgcaata aaatcccgct ggtcacgacc ggtggcgcgg gtgggcagat
     3181 tgacccgacg cagattcagg ttaccgatct ggcgaaaacg attcaggacc cgctggcggc
     3241 gaagttgcgc gagcgcctga aaagcgattt tggcgtagtg aaaaacagta taggtaagct
     3301 cggcgtggat tgcgtgtttt ctactgaagc gctggtgtac ccgcagtcag acggtacggt
     3361 gtgtgcgatg aaagccacgg cagaagggcc gaagcggatg gattgtgcat ctggatttgg
     3421 cgcggcaacg atggtgaccg ccacctttgg gtttgttgcg gtttctcatg cgctgaagaa
     3481 gatgatggcg aaagcagcgc gtcagggtta aacaatcagg tcggatgcga cgcgaacgtc
     3541 ttatccgacc tggttctgtg cttaaacctg cttcgccgca gcgataattg cctcgcttaa
     3601 cgcattcaac ccctggctgc gtgaggcgct aagctgccca cgcaatccca gctcatcaaa
     3661 caatgccagc ggcgactgtg cctgtaattc ggcggcggtt ttcccctcaa cggcagtcaa
     3721 caacaccgcc aacaggccga gcacaatgcg cccttcgctg tcgccaaaga agtgcatttt
     3781 accgttttca gccacggtat atcccagcca gacgcggttt tcgcatccgg caatctcttt
     3841 agcctgcgct tttaactcgt ctggcaaagc cggaagctgt ttccccagca tgatcaactg
     3901 gcgatattta tcttcccatt gcgtcaacgg tgcgaaggta ttgcgtaacg tttctgcggt
     3961 tacggttgtg ccgaacggat gtccggcgaa ttgcgggttt gtcattaatc caccaataat
     4021 tccagcgcgc ggtcaacggc attcaccagc gcatccacat cactctttgt attatatggc
     4081 gcaaaagagg cgcgcagtgt gccggttacg cctaattctg ccagtagcgg ctgagcacaa
     4141 tgttgcccgg cccgcaaggc aataccgtac tccgccagta gcgtcaccat atcgctgtga
     4201 tgaacgccag caaaatcaaa ggccagcagg ctggaatcct ggcagcggaa tgaacgaaag
     4261 cctggacgtt tcgccagcgc atcttccgcc agcgttgcta agctatggct ccagttttcg
     4321 gcctggttga tatcgtaatc tgccagccat tccagcgccg cgcttaatcc tatgacacca
     4381 gccacatttg gcgttcctgc ttccagtttc cacggcgcag attgagtcgt gaagccgtca
     4441 aaactcactt cgtgaatcat tttgccgcca cccagccagg gcgacatcgc ttccagcagt
     4501 tctgatttgc catacagcac gccgataccc gtcgggccat acagtttgtg accagagaaa
     4561 gcatagaaat cgatatccag ttgctgaaca tccgcgggga aatgcaccgc cccctgagca
     4621 ccatcaacca tcaccaccat ccctgctgaa tgagcaaagg taatcgctcg cgccagatcc
     4681 gggcaaccgc cagtaacgtt cgacatctga ctcaacgcca gaatccgact acggggagta
     4741 atcagttctg gcaacaaatc gacatccggc agtcgctgcg cattaagcgg caatttcacc
     4801 actttggctc cagtttgttg ggcgaccatc agccagggga cgaggttggc gtggtgttct
     4861 gccacgctga caataatctc atcgcccggt tgcagacgcg gacgcgcata gcattgtgcc
     4921 accatgttga tggattcagt ggtgccgcgc gtccagacga tagttttatc atccggtgca
     4981 ttcagtaatt gcgccacttt ctctcgtgca gcttcataac gcgcggtcag gcgttgggct
     5041 tcggcaaact ggctgcgatg gacgtttccg gcgctcaggc tgtaaaactg tcgagtggct
     5101 tcaaccacgg cttcaggttt aagcgcggtc gcggcgctgt cgagatagac gcccgcatcc
     5161 tgtagtgcgg gaaactgggc gcgaaactgc gcgggattaa aaacgttcat ggtactcctc
     5221 ggcttgatgc accgatcgtg acgcaaaatt cactttgata caaggagagt gctaaccatc
     5281 agaattatct ggatatcctg gcgaagccgc gtaagtaaca tatgctgaat agtgttaggc
     5341 gttaatttta gcctgttaat caatactatt aagatttatt aatcgcttta aggagaagat
     5401 gatgaaaaag actgccgcaa ttatttctgc ctgtatgctg acttttgccc tgagcgcctg
     5461 ttccggttcg aactatgtga tgcacaccaa tgacggacgt accatcgtct ctgacggcaa
     5521 accacagact gataacgata ccggtatgat ttcgtataaa gacgctaatg gcaacaaaca
     5581 gcagatcaac cgtactgacg tgaaagagat ggtcgaactg gatcagtaat tcgcgatcgc
     5641 aaggtaaaaa aaagcaccgc aattaggcgg tgctacatta atcactatgg acagacaggg
     5701 taaatgtaca ggaagtgaaa aaaggtagct ttgctaccat ggtctgaatc gcagaccaat
     5761 tgcaaacaca acaacacaac atcacaaccg taagccaaaa gttcaccaga acacgcattc
     5821 cgataaaact tttcgttccg gctcaggaag tgccgccact ataggtattt gctggtagaa
     5881 gctcaacgga caatttataa tggctcagat taaaaaaact aataggttac atagtgtgat
     5941 ctaattgtta aattcattta acatcaaagt ttaatagcca tgtctaaacg attaccaccg
     6001 ctaaatgcct tacgagtttt tgatgccgca gcacgccatt taagtttcac tcgcgcagca
     6061 gaagagcttt ttgtgaccca ggccgcagta agtcatcaaa tcaagtctct tgaggatttt
     6121 ttggggctaa aactgttccg ccgccgtaat cgttcactcc tgctgaccga ggaagggcaa
     6181 agctatttcc tcgatatcaa agagatattt tcgcaattaa ccgaagcgac gcgtaaactc
     6241 caggcccgta gcgccaaggg ggcgttgacg gtcagtttac tccccagttt cgccattcat
     6301 tggttggttc cgcgactttc cagctttaat tcagcttatc cgggaattga cgttcgaatc
     6361 caggcggttg atcgtcagga agataagctg gcggatgatg ttgatgtggc gatattttat
     6421 ggtcggggca actggccggg gctacgggtg gaaaaactgt acgccgaata tttattgccg
     6481 gtgtgttcgc cgctactgct gactggcgaa aaacccttga agacaccgga agatctggct
     6541 aaacatacgt tattacatga tgcttcgcgc cgtgactggc agacatatac ccgacagttg
     6601 gggttaaatc atatcaacgt tcagcaaggg ccaattttta gccatagcgc catggtgctg
     6661 caagcggcta tccacgggca gggagtggcg ctggcaaata acgtgatggc gcaatctgaa
     6721 atcgaggccg gacgtcttgt ttgcccgttt aatgatgttc tggtcagtaa aaacgctttt
     6781 tatctggttt gtcatgacag ccaggcagaa ctgggtaaaa tagccgcctt tcgccaatgg
     6841 atcctggcga aagccgctgc tgaacaagaa aaattccgct ttcgttatga acaataattt
     6901 acgtagggta cgaccatgac cagccgtttt atgctgattt tcgccgccat tagcggcttc
     6961 atttttgtgg ctctgggcgc ttttggcgcg catgtgttaa gtaaaaccat gggggccgtt
     7021 gagatgggct ggatccagac cggcctcgaa taccaggcgt ttcatacgct ggcgatctta
     7081 ggtctggcgg tggcaatgca gcgtcgcatc agtatctggt tttactggag tagcgttttc
     7141 ctcgcgttag gcacggtgtt gttcagcggc agcctttatt gcctggcgct gtcccatctg
     7201 cgtttgtggg cgtttgtcac tccggttggc ggcgtgagct tcctcgcggg ctgggcgtta
     7261 atgttagttg gtgctatccg tttaaagcgc aagggcgtaa gtcatgaata aggttgtatt
     7321 gctgtgccgt ccgggctttg aaaaagagtg cgccgcagaa attaccgata aagccggcca
     7381 gcgggaaatt ttcggttttg cccgcgtgaa agagaattcg ggttatgtca tttatgaatg
     7441 ttatcaacct gatgatggcg ataagttaat ccgtgagctg ccgttcagtt cattaatttt
     7501 tgcccgccag tggtttgtgg tgggggaact cctgcagcat ttgccgccag aagatcgtat
     7561 tacccccatt gtcggcatgt tacagggcgt agtagagaag ggcggtgaac tgcgtgttga
     7621 agttgccgat accaacgaaa gcaaagagtt gctgaaattc tgccgtaaat ttactgttcc
     7681 gctacgcgct gccttgcgcg atgccggggt gctggcgaac tatgaaacgc cgaagcgtcc
     7741 ggttgtgcat gtattcttta ttgcaccagg ctgctgctat accggttact catacagcaa
     7801 caataattcg ccgttctata tgggcattcc gcgcctgaaa tttccggcag aggcgccgag
     7861 tcgttccacg ctcaaactgg aagaggcatt tcatgtgttt attcctgcgg atgagtggga
     7921 tgaacgcctg gcgaacggga tgtgggcggt ggatttaggc gcttgccctg gcggctggac
     7981 ctaccaactg gtgaagcgca acatgtgggt ttattccgtc gacaacggcc cgatggcgca
     8041 aagtctgatg gataccgggc aggtgacgtg gctgcgggaa gacggtttca aattccgtcc
     8101 gacacgcagc aatatctcct ggatggtatg cgatatggtt gaaaaaccgg ctaaagttgc
     8161 ggcattgatg gcgcagtggc tggttaatgg ctggtgccgt gaaaccattt tcaatctcaa
     8221 actgccgatg aaaaaacgct acgaagaagt atcacacaat ctggcgtata ttcaggcaca
     8281 gcttgatgaa catggcataa atgctcagat tcaggcgcgg cagttgtatc acgatcgcga
     8341 agaagtgacg gtgcacgtcc gccgcatctg ggctgcggtg ggtggtcgtc gcgacgagcg
     8401 ataacaggca aggccggggc gtcgtcttcg gcctgctacc ctttccgtca ggctgttacc
     8461 aaagaagttg caacctggcg ctcatcagaa ataatgtgcg ttacctcatc cagatgcccg
     8521 atgcgggctt cccctgaacg attaaattta cttttatcaa tcaataacaa cgattgcgcg
     8581 gcacgtttta atagcatcga tttgtaatca gcgttgatcg cattggagtc ccacagtgcg
     8641 ccgctgctat cgatcccttc acaagaaaaa ataaacagat cgatttccag cgatttaagt
     8701 tgggaaatca gcgagggatt gacgtaacag ccatatttgc gctcaagtgt gccgccggaa
     8761 ctgatcagtt gaatgcgttc gcgtttaccg agttcatagc aaatcggatg gctattggtg
     8821 aagacctgaa tgttgatgtc aggcaactgg cgtgccagat accagcaagt tgaactggcg
     8881 tctaaggcta tcaccatccc ttcttcaatc cacgcgagcg cctcgcgcgc gatatctgct
     8941 ttatgcgcat aatggctttt cagcctgatg tgaaagggat cgccactgtc ttgattttga
     9001 cggtggatat atttagcgcg tccatgattg cgcagaattt taccctgcgt ctgtaattca
     9061 ttgagatcgc gacgaatggt ttctttactg acctttagct gttcagagag agcttccgtg
     9121 gtcaggctgg tatggttcag cagcaggtcg actatcgctt gctggcgtgc cgctttcata
     9181 tttttcaccc catcattcga taaccgcccg cgtcatgcgg gcgtaccggt atgagattac
     9241 ggtgttaccc ctttttttaa aagaatattc ccgtacttcg ctcgttcgcc tgtgataacg
     9301 atcgcaaagg ctttttgcgc ccgttcataa aacgcaaaac gattgatgcg gatgatgtca
     9361 ggacacgggg cttgtagtga aagcgcatta cggtaacgtc gttctacttc aggatcgaga
     9421 gtgtcacctt ctaccgccgc catcatcacc agcggcggtg cataactgtc cagttcaaat
     9481 aacgggataa tcgcctggag caagtcgctc accaacaggc catcagcgcg gatcacctgc
     9541 ggtcccatcg aatgggcggg aaagtgagca tcggaaaaaa taatttcatc tccatgtccc
     9601 atctctgcca gcactttcaa tagttcggga gaaattaacg gcgaaattgt tttcagcatt
     9661 tcacacttcc tctataaatt caggttcagt ttgcgggtag aaataacggt actgataatg
     9721 aatctgtgcg cgggcttctt cagggctgtt aaattcccct acgccatacc aaccgaacag
     9781 cgcagctcct gcgacggtcg tttcggcgtc gtcgagaact tttaccggaa tatcaagcat
     9841 attggcttta atctgattcc acaatgtgtt gcgacttcct ccaccgacta acaataattc
     9901 agaggcctta aagtgcccga ttttttccag catctgtaga ttgcgctgta attgcgcagt
     9961 taacccttcc agcgccgcgc gatagaaatg cccccgcgtg gtattaagcg tcactccttg
    10021 ccagccagcg ttctgacacg acaataaatc acactgcatt tttacgccat ccgcgctagg
    10081 cgcgataaga cgagcttctt caatcaacat ttgccagggt gtttcagccg tccagaacag
    10141 ttttctcacc cattccagca cgccggatgc cagccattgc atacctgggt tatacaaccc
    10201 tgcctggcta tccagttcgc aggtggaacc ggcgtactga cttaacagcg aagtatcaac
    10261 ctgggcgcta cggaccatta aaatttccca tgtaccggaa gagagcacgg gttcattttg
    10321 gtcggcacca gcgccaaaaa gggcgaactg ggtatcgtga cctgcggaaa tcaccggtat
    10381 gccaacgggt aagccgagca ttgctgcggc gctgttctgt agcgtaccaa tctgttcacc
    10441 cgcttccacc agacgaggga agagtcggcg tggaatacca gtggcttgta aaatttgcgg
    10501 actgaaatcg cgttgctgga tatccagcat ctggctggtt ccggccatcg tgatatcagt
    10561 agtgaattcg ccggttaaac ggtggttaat cagcgacgaa ataaagaacc aggcgtgcgt
    10621 gcgttccagc agttgtggat gattttcttt caaccacacc aacttatata acgtattgaa
    10681 actaaaggct ccgacgccag aaatagcctg caaccgctgt gcggagatca actgttcaat
    10741 attgtccata accgctgctg ttcgcggaca tttccagcta atgatcggat agagcagatt
    10801 gccttgctta tctaccagag cgccatccac accaaaggtg gtgacggcga taccgcggat
    10861 gtggcattca gtcagttcac tattgatttg ccgacagcaa tcagcaaagc gttgcaaaat
    10921 ggcgtctaaa gaccactggt gccaggtgtt gttttccatc gcgatatcgc tggcattagg
    10981 cgttgaggcg cgggcaacaa tttttccctg ccgattaacc gcgatggccc tgacattggt
    11041 cgcgccacag tcgagtacca ggataacttc ttgtttcata atcgctcctg caagacttca
    11101 atatccatgc cgtgcgcagc ccaggcagaa ggacgataca ctttggtctc ttcaacgttg
    11161 tgcatacata ccgggatacg cagcatggag gcgagagtga taaagtcagc gccaacgtgg
    11221 ccgatggtca gaaccccatg gttagcgccc cagttcgcca ttaccgagta cacatccgta
    11281 aacggccctt taccggtgag gcgcggtgca aaccaggtgg ttggccaggt tgagttggtg
    11341 cgtttgttga ggatgtcatg cacatccttc ggcaattcca cgctccagcc ttccgcgatt
    11401 tgcagtaccg gtcccaggcc tttgatgatg ttgacacgag tcatggtgaa cgggacgccg
    11461 ccttcggtaa ggaagcggga agagtaaccg ccgccacgga agtattcgtg gatcgccggg
    11521 caccattcgg tagcggcgag gcaagcgtca gcctcttgct gagagatttc ccagtgcggc
    11581 ttcatcgtcg ggttaccttc gctgtcgcgt tgtttacagg aaccgtccag cgcagcagaa
    11641 cctgagttga tcaaatggat gatgccgtgt tctgccagtc catcgagttt atgccccgtt
    11701 acacgctcaa ttgcttctgg tgaccagtag gtacgcacat cggcaaatac ctgagcggtg
    11761 ccggtgagct ggtgacccat tagcattgcc acgccgttaa gactgtcgtt ttcggtcgcc
    11821 acgacaaagg gttcgcgcac gccattccag tcaaatgaac tgttgaggat cgcttcggcg
    11881 gtgtcaccat tgggatattg atcggtccag tgacgttgcc cctggaagcc cgcagcgatg
    11941 gcgttgtagc caagtgattc ttccacgcga ccaatatcgg ccagtttgct gttgccttgc
    12001 atcatgtcgc ggatacacat cgccatcagt aaactttcgc gcagaactgc gcggctttgc
    12061 tcggcattgc gttgatactg tttgttattt tcatcttcgc catagcggaa gtttttatca
    12121 gcccaggcca gtgccatttc cagttcggct tcgtcgtaaa tcttctgatc gatacggcga
    12181 cgcagttcgg tcatatccac cgcctggact ttcattccca gccaggattc aaagaagttg
    12241 tgatcaacga tggaaccggc gatacccatc gaaacgccgc ccagcgacag atagctttta
    12301 cctttcatgc tggcgacggc caaaccggcg cgggcaaagc gcagcagttt ttcttcaaca
    12361 tcggcaggaa tcgatgtgtc atcggcatcc tgaacgtcat gaccgtaaat ggagaatgcc
    12421 gggatgcctt tctggctgtg agctgccaga gccgctgcca ggtaaacagc gccgggacgt
    12481 tcagtgccgt taaagcccca aatggctttc gggcgggttg gatccatgtc gatggtttca
    12541 ctgccatagc accagcaagg cgttaccgta atggtgaggc ctacattctg gctgctgaat
    12601 ttttcttcgc aagcagcggc ttcagccata cccgcgatac aggtatcgga aatgacacac
    12661 tcgacggcag ctccgcaggc atggcgcagt ttctcggtca gcagtgcggc cgtagctttc
    12721 gccatattca ttgtttgttc ttcaagcgac tcacgaacac ccatgcgacg accgtcaata
    12781 accgggcgga taccaatttt cggtaagctg atttttttca ttacagattc ctcactttat
    12841 tcggaaaata tgttcagtta gttgccgttt gagagcggaa acgggcaaag ataaagatga
    12901 ccgcgaagca gagtgcgggg atcagttcag cagtggggat gttgcccgcc gcgtcactga
    12961 caaaacccat gaccggagtg acaataccgc cgccaataat ggtcataacg atgaaggacg
    13021 aaccgtattt ggtgtcctgg ccgagattct taatgcccag cgagaagatt gttgggtact
    13081 gaatcgacat aaaggcgctg cataaagtca gggctattaa gcccacatga ccgccagcga
    13141 aggctgagat caggcacagt gccatagcga ttaatgcgta ggcggccagg actttgtgtg
    13201 gtgcgaagcg actgatgagc caggtaccgg tgaaacgacc aataaagaag cacaccatgg
    13261 ttccggttaa atagttagcg gcaaagcctg cagtcatacc tggaatttct tctacagcgt
    13321 agcgaatcaa atagctccag caggccgttt gtgcgccaac gtagcagaat tgcgctaata
    13381 ctgcccagcg ccagtggcga atacgcgcca ggcgagaaag cgatgcggag aacgatcctt
    13441 gtttggcgtc actgtgatta tcactctgca atgccgggaa tttcgtcagc atgatcagca
    13501 gggcgaccag taacacgata gccacgatga tcatataagg tgtctgtacc gataatacca
    13561 ggctgtgttt atacgcactc aattgctctg gagacatttt atcgagaacg tcttgcgatt
    13621 gatgtggcac gttagacaaa ataagacttt gcccaaagac aaccgcgata attgcgccaa
    13681 acgagttaaa tgtttgcgca agatttaagc ggaagtgacc actgctttcc ggccctaata
    13741 ccgtaacaaa agggtttgcg gcagtttcca gacaacctaa tccggctgca ataataaata
    13801 ggccaactaa aaataaggtg tagttcatta tttctgcagc gggccagaat aatgcagcgc
    13861 ccagggcata taaaaataac ccagtaata
//
LOCUS       DAJVUJ010000109        13850 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.109, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000109 DAJVUJ010000000
VERSION     DAJVUJ010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13850
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.109"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(<1..565)
                     /locus_tag="OQQ48_002886"
     CDS             complement(<1..565)
                     /locus_tag="OQQ48_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1953585.1"
                     /translation="MSTTTRKFKTIITDTGAKKLAQVAAPDGKPVRLTHMAVGDGGGT
                     LPTPDSKQTRLVHEVWRHTVNRVILDATHQNRIIAELVIPPETGGFWIREIGVFDEHG
                     DLIAVGNTAESYKPAVAEGSGRAQTFRTILTVSSTATVALTVDNTMVMATVDYVDDKL
                     KEHEQSRRHPDASLTAKGFVQLSSATNS"
     gene            complement(568..1098)
                     /locus_tag="OQQ48_002887"
     CDS             complement(568..1098)
                     /locus_tag="OQQ48_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HCS1953586.1"
                     /translation="MSNSLLPPSASNFMRCAEAVGTRITDIPVDLNTLWSPDTCPVHL
                     LPYLAWAFSVDRWDRNWPEETKRQVIRDAWLIHRHKGTISALRRAIEPLGYLIRVSEW
                     WEFGGEPGTFTVEVGTLDSGVTEEMYLEMERLIADARPVSRHMTGLNIIQEIPGDIFA
                     AAATYDGEVITIYPGD"
     gene            complement(1091..1987)
                     /locus_tag="OQQ48_002888"
     CDS             complement(1091..1987)
                     /locus_tag="OQQ48_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HCS1953587.1"
                     /translation="MPAVDLSQLPEPAIIAEPDFEAILADTKAMMIASYPAEQREAVS
                     AALELESEPLNVIAQTMSFREMLLRQRVNEGARACMLSHSAGTDLDNLAGNMNTKRLV
                     ITPATDTTDAVMESDTSLRLRAQRAYDGLSVAGPSGAYEYFARSASGLVRDARAISPS
                     PANVTVSILSTEGDGTATEALLNTVRAVLNAEDTRPVADRLTVQSARIVTWRLNAKLY
                     FYPGPESEPILAAAESSFRKWLAEQGLIGQDVALSAIAAALHVHGVQRVEIIEPTQNM
                     AISDIQAARCESFTISEGGRNE"
     gene            complement(1991..2320)
                     /locus_tag="OQQ48_002889"
     CDS             complement(1991..2320)
                     /locus_tag="OQQ48_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001705832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HCS1953588.1"
                     /translation="MNPDGTGNLNDMEHLKQSVRDILTTPLASRVMRREYGSLVPDLI
                     DEPMNNTTRLQCMSAAVIALTRWEPRIALDAIDVVWKAGGRAGVTLSGTVMQTMQNVE
                     LTITLRE"
     gene            complement(2338..2904)
                     /locus_tag="OQQ48_002890"
     CDS             complement(2338..2904)
                     /locus_tag="OQQ48_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HCS1953589.1"
                     /translation="MRLLSNIIRTGIISEVDEKSWRVRVRSGELETGWLRWNTSRAGA
                     FNVWLPPSPGEQVVIACIGGNPETAMIIGSLWSDAIPAPGKSLKEIVVSAPDGAVFRY
                     DADAGALSASGMKTATLQASVSVTLDTPVVECTDLLRTATLDVTKGGKMSGNITHSGG
                     NFTSNGITVHTHKHGGVKGGSDSTGGPQ"
     gene            complement(2916..3551)
                     /locus_tag="OQQ48_002891"
     CDS             complement(2916..3551)
                     /locus_tag="OQQ48_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HCS1953590.1"
                     /translation="MDKAFTRVDETFEAIRDSLNQQAINNIARKLAQDLRRAQQARIR
                     PQKAPDGTAWTPRRRHVTRIQERIRFIWNNEARTLKNWHHDTGKYGRTITGWDEDKNS
                     IRTFYRDDIDRFLEIRTRRINQDSTKRVPMFVKLRTARYLKARADASGVTVGYSGVAA
                     RIARVHQFGERDQVAPGIFTDYPVRELLGISQADERLIYNTVLGRIAEAVR"
     gene            complement(3544..4011)
                     /locus_tag="OQQ48_002892"
     CDS             complement(3544..4011)
                     /locus_tag="OQQ48_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1953591.1"
                     /translation="MLKTDSLREAMTRSCRWCQANPEKFTIFVESGNIETTGETPSFV
                     YRYQMVMFVMDYAGELDDLTLPLLAWLSENQPQLLLNPERNQDIKFSAVINDDDSADL
                     LFTLPLRERVRITRSSQGTPQAEHLLEPKPRLPSSEGDWSHVFQDVTWGESDG"
     gene            complement(4149..4556)
                     /locus_tag="OQQ48_002893"
     CDS             complement(4149..4556)
                     /locus_tag="OQQ48_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2570 domain-containing protein"
                     /protein_id="HCS1953592.1"
                     /translation="MKLWPTLGVAFLLTAGWGTSMRLSWSLGRENARNEAQASTLKST
                     ADTLNIISAGVQDMQQVLAQLRVENQQRNQDGEVRREQLRNDIAKDECAHALPDARFT
                     DRLRRHAERATASAVSPAYTADADHTGNASPLP"
     gene            complement(4553..4945)
                     /locus_tag="OQQ48_002894"
     CDS             complement(4553..4945)
                     /locus_tag="OQQ48_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HCS1953593.1"
                     /translation="MSNTFKFSSRSEKNLQGVNPDLVKVTRRALEISEVDFGITEGLR
                     SRYRQKQLVATGKSQTMNSRHLTGHAVDVVAYIGSQMSWEWPLYEKIAAAFRQASREL
                     NIPVEWGGDWKTLKDGPHFQLPHGAYPA"
     gene            complement(4942..5265)
                     /locus_tag="OQQ48_002895"
     CDS             complement(4942..5265)
                     /locus_tag="OQQ48_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCS1953594.1"
                     /translation="MSTIQTGITEQVIAWLFDHLPTVYAVGAAVSISALMSLYDGRTL
                     VQTVTGSLACGVLAMAVAGSLRFFGFPEDAVTFIGASIGFMGAEKARDKVIAAFNRRV
                     KEKDE"
     gene            complement(5268..5468)
                     /locus_tag="OQQ48_002896"
     CDS             complement(5268..5468)
                     /locus_tag="OQQ48_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HCS1953595.1"
                     /translation="MKVKALEGDTVDSLCFRYYGTTQGVTEKVLDANPGLCQQVFLDA
                     GQEVEMPEPEKKKREMIQLWGE"
     gene            complement(5468..5962)
                     /locus_tag="OQQ48_002897"
     CDS             complement(5468..5962)
                     /locus_tag="OQQ48_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HCS1953596.1"
                     /translation="MTIVARTEPGPAEDDITDTDDGDTRISAGAFWPDIVLRELRLAV
                     RLPGRVTTSRLLHTATGAVAHVTRELEAWQQEQQAAGHQTLADVPAPVINGESVNLWH
                     WRNAVYTATRALILERYRDADTTDKGDRRADALDIQTSDLWRDVSWAISDILCRPRIF
                     AELC"
     gene            complement(6130..6876)
                     /locus_tag="OQQ48_002898"
     CDS             complement(6130..6876)
                     /locus_tag="OQQ48_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004983471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HCS1953597.1"
                     /translation="MITPAQQHWQNVMAQRAGRANEGVDHAARTAHEEVLYRLRLAQA
                     RLKGVQARSAKAAIKKELLPDFSGWIEGTLEADGGQQDEVIATLMVWAIDCGDLPLAL
                     RIGAYVVRHNLIMPDNFGRTAATVLTEEICNPVLTQAGTDADADLSAFIEPLDTLREI
                     VTDQDMPDEVRAKLCKACAFARRGLSDADSMALSLKLLREAMHLNPNAGVKREIATLS
                     RALKKADSAAEPEDASAQQAQDESSKKQQK"
     gene            complement(6922..7794)
                     /locus_tag="OQQ48_002899"
     CDS             complement(6922..7794)
                     /locus_tag="OQQ48_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005136493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P2 family phage major capsid protein"
                     /protein_id="HCS1953598.1"
                     /translation="MIGVTDQKGEKILLDTTGPIARTNTSYDGTKRRNPNNVVDLKNR
                     KYQCEQVNYDTFISYPQLDAWSAHPDFQSRISAQIARQVALDRIMIGFNGTSHADESN
                     FSTNKLLQDVNVGWLEHIRTDASERVMNDVTLTSRNMDNTVAHAGKYANADALVQDAR
                     SSLLDEWHKEADDLVVIMGRNLFNSLRLPVLNSISGQNPNAELLAGQLILSSRTIGGL
                     GVFLAPFFPDATMLITSFNNLSIYWQKGSMRRLMKDEPEYNRIATYQSINDAYVVEDY
                     GKCAMVTGLKFADS"
     gene            complement(7791..7973)
                     /locus_tag="OQQ48_002900"
     CDS             complement(7791..7973)
                     /locus_tag="OQQ48_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P2 family phage major capsid protein"
                     /protein_id="HCS1953599.1"
                     /translation="MNLVMSDITRNKLGCYMAQQVSLNNIPVSALVSRFTVEPAVQQR
                     FENASKESTEFTKKIT"
     gene            complement(7997..8833)
                     /locus_tag="OQQ48_002901"
     CDS             complement(7997..8833)
                     /locus_tag="OQQ48_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HCS1953600.1"
                     /translation="MANEKKTSRKKFRVAVSGSTVDGREISPVHLREAAENFNPDVYA
                     ARVNVEHYLSPCPSSEFSAMGDVTALSTEEITEGPLAGRTALYAEIEPTERMKQLVAD
                     GKKIYSSIELHPQFSVNGRAYLVGLAMTDTPASLGTERLKFTAQQRQAVMTFNSIQGE
                     APLISEAIESEIIEMAEQRQEEGTQWFNRVMGIIGRGRKADDASFSRIQEAVEGVATS
                     QADIIDRFNVLETRHQQDRQKITSLTTELAALKEKLRTQDGDPQNRFTATGAASDQLA
                     DF"
     gene            8988..10739
                     /locus_tag="OQQ48_002902"
     CDS             8988..10739
                     /locus_tag="OQQ48_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HCS1953601.1"
                     /translation="MIQDAFVRQRARQLYWQGYPPAEISRLMGINPNTIYAWKKRDQW
                     DETPPGQRVTQSIDARLIQLTEKQNKTGGDFKEIDLLTRQLKKLHDGQPDVMAAGKKG
                     RAKKLKNHFTPEQIAALREKIISRLEWHQRGWFDSLTLCREAGIRNRMILKSRQIGAT
                     WYFAQEALLMALRDDVAQPYQRNQIFLSASRRQAFQFKSIIQKAAAEVDVELKGGDKI
                     ILSNGAELHFLGTSAASAQSYTGNFYFDEFFWVSRFAELRKVAGAMATLSGLRRTYFS
                     TPSTETHEAYAYWNGDRWNEKKASHKRQRFSVDWKTLHNGLICPDRTWRQIVTLEDVV
                     NHGWKHTDIDEIRDENTEDEFLNLYMCEFVREGESAFNLNILIGCGVDGYDDWKDWKP
                     FAPRPMGNRPVWIGYDANGSSGNGDSGAVSVVVPPSVPGGRFRTVETRRVQGLEFEEQ
                     ARVIEEFTCRYNVEHIGIDVTGGNGEAVYQIVKRFFPAAIPYTFTLSSKRSLVLKMLQ
                     IMRAGRWEYDRAERELVAAFNAVRKVKTPGGFITYETDRARGISHGDLAWATMLAVIN
                     EPIGGEGENERFTVMEF"
     gene            10739..11785
                     /locus_tag="OQQ48_002903"
     CDS             10739..11785
                     /locus_tag="OQQ48_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCS1953602.1"
                     /translation="MSRKNKKVRMSSRIDLADALRKESSLSAFTFDGPYRLTGHDLLD
                     NMYCADNGRWYETPVDWYGLARAARQTSWHQSALYFKRNVLLGCYIPHPLLSRQDFSA
                     LALDWFVFGNAFLELRSNMLGEPLKLRHALAKYMRRGSDLESWRYVQDGKDAFQFRPG
                     KVCHLMNPDINQEIYGMPEYLGALLSASLSHSADMFRKLYYDNGSHAGCIIYIGAAQV
                     NRESMDSLKETLQGARGGGAFKNVLIHAPNGGKEGVQILPFQQITAKDEFMNVKAASR
                     DDVLAAHRVPPQLMGAMPGEKSAFGDVEKAARVYAINELMPVMEAMKHINDWLGEEVI
                     RFNPYALLDIQPTS"
     gene            complement(12276..12866)
                     /locus_tag="OQQ48_002904"
     CDS             complement(12276..12866)
                     /locus_tag="OQQ48_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HCS1953603.1"
                     /translation="MSEINYQALRERYSPVPVPKCPICGEEMSIQRISGAQVVYACSG
                     YGDDGDFKIGRTLADEHYEKSRVTVLDVGDPEVLALLDWLETKDNRIAELEKIATDYA
                     LKFQKAQDALKHAALLHSRTAQQTNNFAVSLPDISEYFINDVFQPLRYERDVERAIIK
                     AGGKALWQEKHEDRTHQSCDVNCGWFSPLTTDKNNT"
     gene            complement(12930..13241)
                     /locus_tag="OQQ48_002905"
     CDS             complement(12930..13241)
                     /locus_tag="OQQ48_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012907691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HCS1953604.1"
                     /translation="MTTPTRRISFYLKPTAVKNEGEACAWLDSLTPEARKSGQRVAFL
                     AGLALLKMNPAEAYRLAAWAGDEALSVTQTRTERPASQPVSTAQITSQMAGNIRALFP
                     E"
     gene            complement(13246..>13850)
                     /locus_tag="OQQ48_002906"
     CDS             complement(13246..>13850)
                     /locus_tag="OQQ48_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HCS1953605.1"
                     /translation="RKKANVMRPVEYQNGEAFTIRNVQVMPESIPAGFKALADMSPFE
                     SLLIVDLGGTTLDVAKVQGQLAGISQVFCDPHVGVSLMADAVLSVMATNGMRTSHHIA
                     NTIIEHRHDEAWLRQHIHNDAHYASLMAVIREKEETLKQRVIRALAVFSGYGRVMVVG
                     GGAEIVAPAIREACGVNATFIADGVPQFALVNGLYAMDKE"
BASE COUNT         2835 a         3863 c         3812 g         3340 t
ORIGIN      
        1 cgctgttagt ggcgctactg agttgaacaa agccttttgc ggtcagcgag gcgtccgggt
       61 gacgtcgtga ctgttcatgc tctttcagtt tgtcatccac gtaatccact gtggccatca
      121 ccatggtgtt gtccacggta agcgccacgg tggcagtgct ggatacggtc agaatggtgc
      181 gaaatgtttg tgcacgaccg gacccttcgg caacggctgg cttgtaactt tcggcagtat
      241 ttcccaccgc gattaaatcg ccgtgctcat caaatacacc aatttcccgg atccagaatc
      301 cgcccgtttc aggaggaata accagctccg caataatgcg gttctgatgt gttgcgtcca
      361 ggatgacgcg attaacagta tgtcgccaca cctcatgcac cagacgggtc tgcttactgt
      421 ctggtgtggg caacgtgccg ccaccgtcgc ccacggccat atgagtcagg cggacaggct
      481 taccatctgg cgcggctacc tgagctaatt tttttgcacc cgtatcggtg ataatggttt
      541 taaattttcg tgttgtggta ctcatgctta atcgcctgga taaatggtaa taacttcacc
      601 gtcataagtt gccgccgctg cgaaaatatc ccccggaatt tcctgaatga tattcagccc
      661 tgtcatgtgg cggctgaccg ggcgggcatc agcaatcaac cgctccattt ccagatacat
      721 ttcctccgtc acgccactgt ccagcgtgcc gacttcaacg gtaaatgttc ccggttctcc
      781 gccgaactcc caccactcag acacgcgaat gaggtatccc agcggctcaa tggcccggcg
      841 cagtgcgctg atggtccctt tgtgtcggtg tatcagccat gcatcacgaa taacctgtcg
      901 ctttgtctct tccggccagt tgcgatccca gcggtcaacg gaaaacgccc aggcgagata
      961 aggcagcaga tgcaccgggc aggtgtccgg cgaccacagc gtgttgaggt ctaccggaat
     1021 gtctgtaatg cgtgttccga cggcttcggc acaacgcatg aaattgctgg ctgatggtgg
     1081 taacaacgaa ttactcattg cgtccacctt cgctgatggt gaatgactca cagcgcgccg
     1141 cctgtatgtc gctgatggcc atattctgtg tgggttcgat tatctccacg cgttgcacac
     1201 cgtgcacatg cagtgcggca gcaatggcgg acaacgccac gtcctgaccg ataagcccct
     1261 gctcagccag ccacttcctg aacgacgatt cagccgcggc cagaataggt tcggattccg
     1321 ggccggggta aaagtacagt tttgcattca gccgccatgt cacgattctg gcactctgta
     1381 cggtcaggcg gtcggccacc gggcgggtat cctctgcatt cagaacggcg cgaacggtat
     1441 taagcaacgc ctccgttgct gtgccgtcgc cctcagtgga caggatggaa accgtcacgt
     1501 tggccggaga cgggctgatg gcccgcgcat cgcgtaccag accgctggcg ctgcgtgcaa
     1561 aatactcgta tgcacctgac gggccagcaa cactcagacc gtcatatgcc cgttgcgccc
     1621 gtagtctcag cgaggtgtca ctttccatca ctgcatcggt ggtatccgtt gccggagtga
     1681 taaccaggcg ctttgtgttc atattgcccg cgaggttgtc caggtctgtc ccggcgctgt
     1741 ggcttagcat gcaggcgcgt gcaccctcat tgacccgctg gcgtaacagc atttcacgaa
     1801 acgacatggt ttgagcgata acgttaaggg gttccgattc cagctccagc gcggcggaaa
     1861 cggcttcacg ctgttcggcg ggataggacg caatcatcat ggcctttgtg tcagccagaa
     1921 ttgcctcaaa gtcaggctcc gcgatgatgg cgggttccgg taactgtgaa aggtcaacgg
     1981 caggcatgat ttactccctc agcgtgatgg ttaattcaac attctgcatg gtctgcatga
     2041 cagtgcccga cagcgtcacc ccggcgcggc ctcctgcctt ccagacaacg tcgatagcgt
     2101 ccagggcaat gcggggttcc catcgtgtca gcgcaatcac ggcagcactc atgcattgca
     2161 gacgcgtggt gttattcatg ggttcgtcaa tcaaatcagg cacaaggctg ccatattccc
     2221 gtcgcataac ccggcttgcc agcggggtgg tcaggatgtc cctgactgac tgtttcaggt
     2281 gctccatatc gttcaggttt cccgttccgt ccgggttcat tcctgtgtag cgggttgtca
     2341 ctgcgggcct cctgtcgaat cgctgccacc tttaacgcca ccgtgtttat gcgtatgcac
     2401 tgtgatgccg tttgaggtga aattgccgcc gctgtgcgtg atattgccgc tcatctttcc
     2461 cccttttgtg acgtcaagcg tcgccgttct cagaaggtct gtgcattcca cgacgggcgt
     2521 atccagtgtc acgctgacgg atgcctgcag ggtggctgtt ttcatgccgc tggcgctcag
     2581 tgcgcctgcg tccgcgtcgt agcggaacac cgcgccatcc ggcgcgctga ccacgatttc
     2641 tttcaggctt ttgccggggg ccggaatggc atcactccac aggctgccaa ttatcatggc
     2701 ggtttccggg ttgccgccaa tgcaggcaat taccacctgt tcgcctggtg atggcggcag
     2761 ccacacattg aaggctcccg cgcgagaggt gttccagcgc agccaacctg tttccagttc
     2821 gccgctgcga acgcgcacgc gccaggactt ctcatcaact tcagagatga tcccggtgcg
     2881 gatgatattg ctcagtagcc gcatgagttc tgcgctcacc gtacagcctc cgcaatccgg
     2941 cccagcaccg tgttataaat caggcgctca tctgcctggc tgatacccag cagctcacgt
     3001 accgggtaat cggtgaaaat gcccggcgca acctgatcgc gctcaccgaa ctgatgaacg
     3061 cgtgcaatac gtgcggccac gccgctgtaa cccaccgtca caccggaagc atctgcacgg
     3121 gctttcaggt agcgggcggt gcgcagtttt acgaacatgg ggacgcgctt tgtgctgtcc
     3181 tggttgatgc gccgggtgcg tatttccaga aagcggtcga tgtcatcccg gtaaaacgtg
     3241 cggatgctgt ttttatcctc atcccacccg gtgatggttc gcccgtattt ccccgtgtcg
     3301 tgatgccagt ttttcagcgt gcgtgcttcg ttattccaga taaagcgaat gcgctcctgt
     3361 atccgggtta cgtggcgtct gcgtggtgtc catgcggtcc cgtccggcgc tttctgtggc
     3421 cggatacgtg cctgctgggc gcggcgtaaa tcctgtgcca gctttctggc gatgttattg
     3481 atggcctgct gattcaggct gtcgcggatg gcctcaaagg tttcatccac gcgagtgaat
     3541 gccttatcca tcgctttcac cccacgtcac atcctggaat acatgcgacc agtcgccttc
     3601 ggaagatggc aggcggggtt ttggctccag caggtgttct gcctgcggtg tgccctgact
     3661 gctgcgcgtg atgcgaacgc gttcccgcag ggggagcgta aacaggagat cggcgctgtc
     3721 atcgtcattg ataacggcgg aaaatttgat gtcctgatta cgctcagggt tgagcaacaa
     3781 ctgtggctga ttttcggata accacgccag cagcggcagc gtgaggtcgt ccagctcccc
     3841 ggcgtaatcc atgacaaaca tcaccatctg atagcggtaa acaaacgatg gggtttctcc
     3901 ggtcgtttca atgttgccgc tctccacgaa aatggtgaat ttttccgggt tagcctgaca
     3961 ccatcggcat gaacgggtca tggcttcacg cagggaatca gttttcagca tggttgttat
     4021 cctcgttgtt cagtcgttgc agcctgcgct gttccagtaa ttcaatggcc cgtttatccg
     4081 cgttacaggt ttccagtgca tccagaaggc ggtcgcccca tataccgaga tttccccatg
     4141 tgggagtgtc agggaagggg ggaggcgtta ccggtatggt cagcgtctgc ggtataagcc
     4201 ggactgacgg cgctggcagt ggcgcgttct gcgtgcctgc gcagcctgtc agtaaaacga
     4261 gcgtcaggca aagcgtgggc gcattcatct tttgcaatat cgttgcgtag ctgttcacgt
     4321 cttacctctc cgtcctgatt gcgttgctga ttttccacgc ggagttgcgc cagcacctgc
     4381 tgcatatcct gtaccccggc gctgatgata ttcagggtgt cggcggtact tttcagggtg
     4441 ctggcctgcg cttcgtttct ggcgttctcc cggcccagcg accacgacag acgcatggat
     4501 gttccccatc cggcagtcag aaggaaagcg acgccaagcg tgggccagag cttcatgccg
     4561 gataggctcc gtgtggtaac tgaaaatgcg gtccgtcttt cagggtcttc cagtcgccgc
     4621 cccattccac cggaatattc agttcccggc tggcctgtct gaatgctgct gcgatttttt
     4681 cgtacagcgg ccattcccat gacatctggc tgccgatata agccacaaca tccacggcat
     4741 gccccgtaag gtggcggctg ttcatggtct ggctcttacc cgtggccacc agttgcttct
     4801 ggcggtaacg gctgcgcaac ccttcggtga taccaaaatc cacttccgag atttccagtg
     4861 cccgtcgggt cactttcacc agatcaggat ttacaccctg caaattcttt tcgctccggc
     4921 tgctgaattt aaatgtgttg ctcattcgtc cttctccttc accctgcgat taaaggccgc
     4981 aataaccttg tcgcgtgctt tctctgcacc cataaaaccg attgatgcgc cgataaacgt
     5041 cacggcatct tcaggaaacc cgaagaagcg caacgacccg gccacggcca tggcaagaac
     5101 gccgcacgcc agcgatcccg ttacggtctg aaccagtgtt cgtccgtcat aaaggctcat
     5161 cagcgcggaa atgctgaccg ccgcgcctac tgcatacacc gttggcaggt ggtcaaagag
     5221 ccacgcaata acctgctctg tgatccctgt ttgaatggtg ctcactgcta ctccccccac
     5281 aactgaatca tttctcgttt cttcttctcc ggctccggca tctccacttc ctgcccggcg
     5341 tccagaaata cctgctgaca gagtccgggg ttggcatcca gcaccttttc ggtgacgccc
     5401 tgcgtcgtgc cgtagtaccg gaaacagagc gaatccacgg tgtcgccttc cagtgccttc
     5461 actttcatca gcacaactcc gcaaagattc gcgggcggca cagaatgtca gagatggccc
     5521 agctcacatc acgccacaaa tccgatgtct gtatatccag agcgtctgcc cggcggtcgc
     5581 ccttgtccgt agtgtctgca tcacgataac gctccagaat cagggcgcgc gtggcggtat
     5641 aaacagcatt gcgccagtgc cagagattga cgctttctcc gttaattacg ggtgccggaa
     5701 catcggccag cgtctgatgg ccagctgcct gctgttcctg ctgccatgct tccagctcgc
     5761 gggtaacgtg tgccacagcc ccggtggcag tatgcagcag gcgggaggtg gtcacgcgcc
     5821 ccggcagtcg taccgccaga cgcagctcgc gcagcacaat atccggccag aatgcacccg
     5881 ctgaaatgcg ggtatcgcca tcatcggtat cggtgatgtc gtcctctgcg ggtccgggtt
     5941 cagttctggc aactatagtc atggggttca ctcctgaaaa aaaatcgggc ggtgggtgcg
     6001 cggtgtaaac ggtcacggag tcaaactgga acaccgcgca cgccgcccgc tgacggggtc
     6061 agtcgttaac cgcgcttcgc cttctgcgtc gcggtggttt ttcgtgttgc aggcttccgc
     6121 gttgtctttt tacttttgct gctttttgct gctttcgtcc tgcgcctgct gtgcgctggc
     6181 gtcttctggt tcggctgcgg aatcggcttt tttcagggcg cgggaaaggg ttgcaatctc
     6241 gcgtttcaca cctgcgttcg ggttcaggtg cattgcttcg cgcagcagct tcagtgacag
     6301 ggccatgctg tccgcatcac tcaggccacg gcgggcaaag gcgcacgctt tgcataattt
     6361 ggcgcgcact tcgtccggca tgtcctggtc ggtgacaatc tcccggaggg tgtccagtgg
     6421 ttcgataaag gcggacaaat ccgcgtcggc atccgtcccg gcctgcgtca gtaccggatt
     6481 acagatttct tcggtcagta ccgtggcagc agtacggcca aagttatccg gcatgatgag
     6541 gttgtgacgg actacatacg cgccgatacg cagcgcaagc ggaagatcgc cacagtcaat
     6601 cgcccacacc atcagcgtgg caatcacttc atcctgctgc ccgccgtcag cctccagcgt
     6661 tccctcaatc cagccggaaa aatccggcaa caactctttt ttgatggcgg ctttcgcgct
     6721 tctggcctgt acgcccttaa gccgggcctg tgccagacgc agacgataca gcacctcttc
     6781 atgcgcggta cgcgcggcgt ggtccacgcc ttcattcgcc cggcctgcgc gctgtgccat
     6841 cacgttctgc cagtgttgtt gtgcaggagt aatcattttt gctctccgtt acaggcgggc
     6901 atgatgcccg ccgtgagttg attagctgtc ggcgaacttc aggccagtaa ccatcgcgca
     6961 cttgccatag tcttcaacga cataagcgtc attgatggac tggtaggtgg cgatgcggtt
     7021 gtattccggc tcgtctttca tcaggcgacg cattgaacct ttctgccagt aaatcgacag
     7081 gttgttgaac gaggtgatca gcatcgttgc atccgggaag aacggcgcaa ggaacacgcc
     7141 cagcccgcca atggtgcgcg atgacaggat gagctgcccg gcgagtaatt ccgcattggg
     7201 attctggccg ctgatgctgt tcagcacggg cagacgcagc gagttaaaca ggttgcgccc
     7261 cataatcacc acgaggtcgt cagcttcctt gtgccattca tccagcaggg atgaacgcgc
     7321 gtcctgtacc agtgcatcag cgttcgcata cttacccgcg tgcgccacgg tgttgtccat
     7381 gttgcgggag gtcagcgtca cgtcattcat aacgcgttcg ctggcgtcgg ttctgatgtg
     7441 ctccagccag cccacgttaa cgtcctgaag cagcttgttg gtgctgaagt tggactcatc
     7501 cgcgtgagac gtgccgttga aaccgatcat gatgcggtca agcgccacct gtcgggcaat
     7561 ctgtgcgctg atgcgggact gaaaatcagg gtgtgccgac caggcatcaa gctgcggata
     7621 cgaaataaac gtgtcgtagt tcacctgttc acactgatat ttgcggtttt tcagatcaac
     7681 cacgttattc gggttacggc gttttgtgcc gtcataactg gtattcgtgc gcgcaatcgg
     7741 tcctgtggta tccaggagga ttttttcgcc tttctggtcg gtcacgccga tcacgttatt
     7801 tttttcgtaa attcggtact ttcctttgag gcgttttcaa aacgctgctg caccgcgggt
     7861 tccacggtaa atcgcgacac cagtgcagat accgggatat tattaagcga cacctgctgc
     7921 gccatatagc aacccagctt gttgcgggta atatctgaca tcaccagatt cataaaaaat
     7981 ttgctccttt gtctaatcag aagtcggcca gctggtcgga ggctgcgccc gttgcggtga
     8041 agcggttctg cggatcgccg tcctgcgtgc gcagtttttc cttcagtgct gccagctctg
     8101 tggtcagtga cgtaattttc tggcggtcct gctgatggcg ggtttccagc acattaaaac
     8161 ggtcgataat gtcggcctgt gacgttgcga cgccttccac cgcttcctga atacgggaaa
     8221 aactggcgtc atccgctttg cggccacggc caataatccc cattacgcgg ttaaaccact
     8281 gggtgccttc ttcctggcgt tgttctgcca tttcgatgat ttcagactcg atggcttcgg
     8341 agataagcgg cgcttcaccc tggatactgt taaacgtcat caccgcctga cgttgctgtg
     8401 ccgtgaattt caggcgctca gtgcccaggc ttgccggggt gtcggtcatc gccagcccga
     8461 ccagataggc gcgcccgtta acggagaact gcgggtgcag ttcgatactg gaatagattt
     8521 tcttgccgtc cgcaacaagc tgcttcatgc gctcggtcgg ttcgatttct gcatacagcg
     8581 cagtacgtcc ggccagcgga ccttccgtaa tctcttccgt actcagtgcg gtgacatcgc
     8641 ccattgcgga aaattcgctt gacgggcatg gcgagagata gtgctcaacg ttcacgcggg
     8701 cagcgtaaac atccgggttg aagttctcgg cggcttcacg cagatgcacc ggactgattt
     8761 cacggccatc aacagttgat ccggagacag ccacgcgaaa ctttttgcgg gatgtctttt
     8821 tttcattagc catagttttt gcccctctga ctggttcttc agtcatgatg gcaaagcgta
     8881 acaggctgat acaaagggct tttgttgtaa gaaaacggcc agaacagagg gttaaggaga
     8941 acagtttcgc gcgcgggtaa tcttcctgta attactcagg gggagcaatg attcaggacg
     9001 cttttgtgcg ccagcgtgcg cggcaacttt actggcaggg ttatccgccc gcagaaatat
     9061 cacgtctgat gggaataaac ccgaacacga tttatgcgtg gaaaaaacgt gaccagtggg
     9121 atgaaacgcc acccgggcag cgtgtcacgc agtccatcga tgcgcgcctc atccagctta
     9181 ctgaaaaaca gaataaaaca ggcggtgact ttaaggaaat agacctgctg acccggcagc
     9241 ttaaaaagct gcatgatggc cagccggatg tgatggccgc aggaaagaaa ggccgggcga
     9301 aaaaactcaa aaatcatttc acgccggaac agattgccgc actgcgggaa aaaatcatca
     9361 gcaggctgga gtggcatcag cggggctggt ttgactccct gaccctttgc agggaagccg
     9421 ggatacgtaa caggatgatc ctgaaatccc gacagattgg ggcgacctgg tattttgcac
     9481 aggaagcact gctgatggcg ctgcgtgacg atgtggcgca accttaccag cgtaaccaga
     9541 tttttttgtc tgcgtcgcgt cgtcaggcgt tccagtttaa aagcattatt cagaaggccg
     9601 cggctgaagt tgatgtggag ctgaaagggg gcgataaaat catcctctcc aacggcgcag
     9661 agctgcattt tctcggcact tctgctgcgt cggcacagtc ctatacgggc aatttttatt
     9721 ttgatgaatt tttctgggtc agtcgctttg ctgaactgcg caaggtggct ggcgctatgg
     9781 caaccctcag cggactgcgg cgcacctact tctccacgcc atccaccgaa acgcacgagg
     9841 catacgccta ctggaacggc gaccgctgga acgagaaaaa ggcctcgcat aaacgccagc
     9901 gtttttctgt ggactggaaa acgctgcata acgggcttat ctgccctgac cggacgtggc
     9961 ggcaaattgt cacgctggaa gatgtggtta atcacggctg gaaacacacc gatatcgacg
    10021 aaattcgtga tgaaaacacc gaagacgagt tcctcaatct ctatatgtgt gagtttgtcc
    10081 gcgaagggga atcggcattt aacctgaata tcctgattgg ctgcggcgtt gacggatacg
    10141 acgactggaa agactggaaa ccttttgctc cccgcccgat ggggaatcgt ccggtatgga
    10201 ttgggtatga cgcaaacggc agcagtggca acggcgacag cggcgctgtg tccgtggtgg
    10261 ttcctccgtc tgttcctggt ggccgttttc gaacggtgga gacgcgacgc gttcaggggc
    10321 tggagtttga agaacaggcc agagtcattg aagagttcac gtgtcgctac aacgtggaac
    10381 acatcggcat tgatgtgacg ggcgggaacg gggaggctgt ttatcagata gtgaaacggt
    10441 ttttccctgc cgctattccg tacaccttca cgctgtcatc aaaacggtcg ctggtactga
    10501 aaatgctgca aataatgcgt gccgggcggt gggaatacga tcgcgccgaa cgcgagctgg
    10561 tcgcggcctt taacgccgtg cgtaaggtga aaacaccggg cggctttatc acttacgaaa
    10621 cggaccgagc gagggggatc agccacggcg accttgcgtg ggcaaccatg cttgctgtca
    10681 ttaacgaacc gattggcggc gaaggagaaa acgagcgttt cacggttatg gagttctgat
    10741 gagcagaaaa aataaaaaag tgcgcatgag ttcacgcatt gatctcgctg atgcgctcag
    10801 gaaagaatca tcgctcagtg cattcacatt tgatggtcct tatcgcctga ccgggcatga
    10861 cctgctggac aatatgtact gtgctgataa cgggcggtgg tatgaaaccc cggtggactg
    10921 gtacggtctg gcaagagctg cccggcaaac gtcctggcat cagtctgcgc tttattttaa
    10981 gcgcaatgta ttgctcggtt gctacatccc gcacccgctg ctttcccggc aggatttctc
    11041 ggcgctggcg ctggactggt ttgtgttcgg taacgcattc cttgagcttc gaagcaatat
    11101 gctcggcgaa ccgcttaaat tacggcacgc cctggcgaaa tacatgcgac gcggaagcga
    11161 tcttgaatca tggaggtatg tgcaggatgg caaggatgcg tttcagtttc gcccaggcaa
    11221 agtgtgtcac ctgatgaatc ctgacattaa ccaggaaatc tacggcatgc cggaatatct
    11281 cggcgcatta ctctcggcca gcctgtctca ttcggcggac atgttcagaa aactgtatta
    11341 cgacaacgga tcccacgccg ggtgcatcat ctacatcggt gccgcgcagg taaaccgcga
    11401 aagcatggac tccctgaaag aaacgctaca gggtgcgcgt ggtggtggtg cgtttaaaaa
    11461 cgtgctcatt catgcgccca acgggggcaa agagggggtg caaattttgc cgttccagca
    11521 gatcaccgca aaggatgagt tcatgaatgt taaggcggca tcccgtgatg atgtgctggc
    11581 tgcgcaccgc gttccgccgc aactgatggg ggcgatgccg ggtgaaaaaa gtgcgtttgg
    11641 tgatgtggag aaggccgcgc gggtttacgc aattaacgag ctgatgcccg tcatggaggc
    11701 catgaagcac atcaatgact ggcttggcga agaggtgatc cgctttaacc cttacgcact
    11761 gttagacatc cagcccacat cctgacgcgc ttcgcttgtc tgctgcttcg ccggggcata
    11821 aaaaatttat gccccgactc tccagctcct gtatcagtca gataatttca cgacgccttc
    11881 cagtttatcg ccaccatcga cggtcagact cttacgcaat cccaccgcgt tgactgcatg
    11941 ttctcgccgc ctcagtgcga ttttgacggc cttacctttc accccatcaa atcagaatcc
    12001 ctcacgtatt tttcacgctc agcgtgagaa atacggccat tctgtcgtgt cgctgcgaca
    12061 tcgttaaggg aacgctattt accccctgaa acgcgggctg ttcccccgtc acctgcgcgc
    12121 agaaaaagcg cgtttttttg tgcacgcacg gatccttgac ggatccagcc gccacgcggg
    12181 ctggaagggc aaaaattcgt tcaaaaaaat tgtgcaaatt tgtgcactat tgtgcagcag
    12241 ggcgatgtgc gattatcgcc ctggtttgag ggggatcagg tgttattttt atctgtcgtc
    12301 agtgggctaa accatccaca atttacatcg catgactgat gcgttctgtc ctcgtgtttc
    12361 tcctgccaca atgcttttcc gccagccttt atgatggctc tttcaacatc ccgctcgtat
    12421 cgcaagggct gaaatacgtc attaatgaaa tattcgctta tgtccggaag cgatactgca
    12481 aaattatttg tttgttgcgc cgtcctgcta tgcagcaaag cggcatgctt taatgcgtcc
    12541 tgtgctttct ggaatttaag tgcatagtca gtggcgattt tttccagttc agcgattcgg
    12601 ttgtctttgg tttccagcca atcaagtagc gccaatactt caggatcccc gacatccaac
    12661 actgttacgc gtgatttttc ataatgttcg tcagcaagag ttcggccaat tttgaaatct
    12721 ccatcatcac cataaccgga gcaggcataa acaacctgtg ctccagatat tcgctgaatt
    12781 gacatttcct cgccgcaaat agggcatttc ggtactggca caggtgaata acgttcacgt
    12841 aatgcctgat agttgatttc gctcattaag tcgttctcct tgtttatgtt ctgaggtgga
    12901 gagcagggcg aactcgccct gatgttgtgt tattcaggaa ataacgcccg gatatttccg
    12961 gccatctgac tggttatctg tgcggttgat actggctgtg atgcggggcg ttctgtcctg
    13021 gtttgtgtca ctgataacgc ttcatcacca gcccatgcag ccagtcgata agcctctgcc
    13081 ggattcattt tcagaagtgc cagcccggcc agaaaagcca cgcgttggcc gcttttgcgg
    13141 gcttctggtg taaggctgtc cagccaggcg catgcttctc cttcgttctt gacggcggtg
    13201 ggcttcagat agaaacttat ccgtctggtt ggagtcgtca ttggtttact ccttgtccat
    13261 tgcgtacagc ccattaacca gagcaaactg tggcaccccg tccgcgatga aagtcgcatt
    13321 aactccgcag gcttcgcgga tagcgggtgc cacaatctcc gcccctccac cgacaaccat
    13381 cacccgcccg taacccgaaa aaaccgccag cgcgcggatc acgcgttgtt tcagtgtttc
    13441 ttccttttca cgaattaccg ccatcaggct ggcgtaatgc gcgtcattgt ggatgtgctg
    13501 gcgcagccag gcttcatcat ggcgatgttc gataatggta ttggcgatgt ggtgactggt
    13561 gcgcataccg ttagtggcca tcaccgacag tacggcatcg gccatcagag aaacgcccac
    13621 gtgtggatcg caaaacacct ggctgatacc tgccagttgc ccctgaacct ttgccacatc
    13681 cagcgtggtt ccgcccaaat ccacaatcag cagggattca aacggactca tgtcagccag
    13741 tgccttaaag ccagccggaa tggattcagg cataacctgt acgttacgga tagtgaatgc
    13801 ttcgccgttc tggtactcca ccgggcgcat gacgttcgct tttttgcggt
//
LOCUS       DAJVUJ010000110        13773 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.110, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000110 DAJVUJ010000000
VERSION     DAJVUJ010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13773
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.110"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(67..1993)
                     /locus_tag="OQQ48_002907"
                     /pseudo
     CDS             complement(67..1993)
                     /locus_tag="OQQ48_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017264.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
     gene            2400..3098
                     /locus_tag="OQQ48_002908"
     CDS             2400..3098
                     /locus_tag="OQQ48_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HCS1953606.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRISMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPTHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWFA"
     gene            3204..3279
                     /locus_tag="OQQ48_002909"
     tRNA            3204..3279
                     /locus_tag="OQQ48_002909"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3237..3239,aa:Phe,seq:gaa)
     gene            complement(3428..3964)
                     /gene="yghD"
                     /locus_tag="OQQ48_002910"
     CDS             complement(3428..3964)
                     /gene="yghD"
                     /locus_tag="OQQ48_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspM family type II secretion system protein
                     YghD"
                     /protein_id="HCS1953607.1"
                     /translation="MLRDKFIHYFQQWRERQLSRGEHWLAQHLAGRSPREKGMLLAAV
                     VFLFSVGYYVLIWQPLSERIVQQETMLQQLVAMNTRLRSAAPDIIAARKSATTTPAQV
                     SRVISDSASAHSVVIKRIAERGENIQVWIDPVVFNDLLNWLNALDEKYALRVTQIDAS
                     AAEKTGMVNVQRLEFGRG"
     gene            complement(3966..5144)
                     /gene="gspL"
                     /locus_tag="OQQ48_002911"
     CDS             complement(3966..5144)
                     /gene="gspL"
                     /locus_tag="OQQ48_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HCS1953608.1"
                     /translation="MSSILEIFFPLCAADPIHWQRRTPDVEHGIWSDVANEQLQQWLQ
                     TDAIRLYIPGEWISVWQVELPDVARKQIPTILPALLEEELNQDIDELHFAPLNIDQQL
                     ATVAVIHQQHMRNIAQWLQENGITRATVAPDWMSIPCGVMAGDAQRVICRIDECRGWS
                     AGRALAPVMFRAQLNEQDLPLSLTVVGIAPEKLSAWAGADAERLTVTALPAVTTYGEP
                     EGNLLTGPWQPRVSYRKQWARWRVMILPILLILVALAVERGVTLWSVSEQVAQSRTQA
                     EEQFLTLFPEQKRIVNLRSQVTMALKKYRPQADDTRLLAELSAIASTLKSASLSDIEM
                     RGFTFDQKRQILHLQLRAANFASFDKLRSALATDYVVQQDALQKEGDAVSGGVTLRRK
                     "
     gene            complement(5141..6118)
                     /gene="gspK"
                     /locus_tag="OQQ48_002912"
     CDS             complement(5141..6118)
                     /gene="gspK"
                     /locus_tag="OQQ48_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HCS1953609.1"
                     /translation="MITSPPKRGMALVVVLVLLAVMMLVTITLSGRMQQQLGRTRSQQ
                     EYQQALWYSASAESLALSALSLSLKNEKRVHLAQPWASGPRFFPLPQGQIAVTLRDAQ
                     ACFNLNALAQPTTASRPLAVQQLIALISRLDVPAYRAELIAESLWEFIDEDRSVQTRL
                     GREDSEYLARSVPFYAANQPLADISEMRVVQGMDAGLYQKLKPLVCALPMIRQQININ
                     TLDVTQSVILEALFDPWLSPVQARALLQQRPAKGWEDVDQFLAQPLLADVDERTKKQL
                     KTVLSVDSNYFWLRSDITVNEIELTMNSLIVRMGPQHFSVLWHQTGESE"
     gene            complement(6121..6720)
                     /gene="gspJ"
                     /locus_tag="OQQ48_002913"
     CDS             complement(6121..6720)
                     /gene="gspJ"
                     /locus_tag="OQQ48_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HCS1953610.1"
                     /translation="MRRTRAGFTLLEMLVAIAIFASLALMAQQVTNGVTRVNSAVADH
                     DQKLNLMQQTMSFLTHDLTQMMPRPVRGDQGQREPALLAGAGVLASESEGMRFVRGGV
                     VNPLMRLPRSNLLTVGYRIHDGYLERLAWPLTDAAGSVKPTMQKLIPADSLRLQFYDG
                     TRWQESWSSVQAIPVAVRMTLHSPQWGEIERIWLLRGPQ"
     gene            complement(6717..7088)
                     /gene="gspI"
                     /locus_tag="OQQ48_002914"
     CDS             complement(6717..7088)
                     /gene="gspI"
                     /locus_tag="OQQ48_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HCS1953611.1"
                     /translation="MKRGFTLLEVMLVLAIFALAATAVLQIASGALSNQHVLEEKTVA
                     GWVAENQTALLYLMTREQRAVRHQGESDMAGSRWYWRTIPLNTGNALLQAVDIEVSRH
                     EDFSSVIQSRRAWFSAVGGQQ"
     gene            complement(7148..7648)
                     /gene="gspH"
                     /locus_tag="OQQ48_002915"
     CDS             complement(7148..7648)
                     /gene="gspH"
                     /locus_tag="OQQ48_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001115132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HCS1953612.1"
                     /translation="MPERGFTLLEIMLVIFLIGLASAGVVQTFATDSEPPAKKAAQDF
                     LTRFAQFKDRAVIEGQTLGVLIDPPGYQFMQRRQGQWLPVSATRLSAQVTVPKQVQML
                     LQPGSDIWQKEYALELQRRRLTLHDIELELQKEAKKKTPQIRFSPFEPATPFTLRFYS
                     AAQNAC"
     gene            complement(7652..8107)
                     /gene="gspG"
                     /locus_tag="OQQ48_002916"
     CDS             complement(7652..8107)
                     /gene="gspG"
                     /locus_tag="OQQ48_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HCS1953613.1"
                     /translation="MNSLSRTHKPRAGFTLLEVMVVIVILGVLASLVVPNLLGNKEKA
                     NRQKAISDIVALENALDMYRLDNGRYPTTEQGLEALIQQPANMADSRNYRTGGYIKRL
                     PKDPWGNDYQYLSPGEKGLFDVYTLGADGQENGEGAGADIGNWNLQEFQ"
     gene            complement(8124..9347)
                     /gene="gspF"
                     /locus_tag="OQQ48_002917"
     CDS             complement(8124..9347)
                     /gene="gspF"
                     /locus_tag="OQQ48_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HCS1953614.1"
                     /translation="MALFYYQALERNGRKTKGMIEADSARHARQLLRGKELIPVHIEA
                     RMNASAGGMLQRRRHAHRRVAAADLALFTRQLATLVQAAMPLETCLQAVSEQSEKLHV
                     KSLGMALRSRIQEGYTLSDSLREHPRVFDSLFCSMVAAGEKSGHLDVVLNRLADYTEQ
                     RQRLKSRLLQAMLYPLVMLVVATGVVTILLTAVVPKIIEQFDHLGHALPSSTRALIAM
                     SDALQASGVYWLAGLLALLVLGQWLLKNPTMRLRWDKTLLRLPVTGRVARGLNTARFS
                     RTLSILTASSVPLLEGIQTAAAVSANRYVEQQLLLAADRVREGSSLRAALAELRLFPP
                     MMLYIIASGEQSGELETMLEQAAVNQEREFDTQVGLALGLFEPALVVMMAGVVLFIVI
                     AILEPMLQLNNMVGM"
     gene            complement(9347..10839)
                     /gene="gspE"
                     /locus_tag="OQQ48_002918"
                     /pseudo
     CDS             complement(9347..10839)
                     /gene="gspE"
                     /locus_tag="OQQ48_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525635.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
     gene            complement(10839..12899)
                     /gene="gspD"
                     /locus_tag="OQQ48_002919"
                     /pseudo
     CDS             complement(10839..12899)
                     /gene="gspD"
                     /locus_tag="OQQ48_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234996.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
     gene            complement(12929..13758)
                     /gene="gspC"
                     /locus_tag="OQQ48_002920"
                     /pseudo
     CDS             complement(12929..13758)
                     /gene="gspC"
                     /locus_tag="OQQ48_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238556.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
BASE COUNT         3099 a         3964 c         3546 g         3164 t
ORIGIN      
        1 tgtaatgcgg tatccaaccc gcgaatatca gcgtgattca ccgtcggatt tactgctacg
       61 tcgccctcag ggaattaaac ctaaaaagaa aatcgtcaaa attagagatt ccggtttgac
      121 acggaaagtc atatcagcgc tatgcgcatc ctgggggttc atcaccactg acgggaaact
      181 ctgctggcgg aacatcgctt tttgtaggtc tttgacgtcg aaactgacat acagatcgtg
      241 catctcctga cacaactggc gcaaagtgta gtcgcgatag cgcaccggat atttgttata
      301 aacgctcggc aacacctcag ccagcggcga gtcatcctca atatgctgtt caaactgcgc
      361 caacatctct accagttgtg ccagcttctc gtggctttcc gccggagtta ataagaacag
      421 aatggagttg agatcgcact tctccggcac aatgccgttc tcacgcagat agtgcgccag
      481 aatcgtcgcc ggaacgccaa agtcgctata ttcgccggtt tcggcattga tacctggtgt
      541 agtgagtaac agcttgcacg gatcaacaaa atactgatcc gcggcatatc cttcaaagcc
      601 gtgccacttc gcccccggct caaaactgaa aaaacggcgg tcgctggcta acactgatgt
      661 cggataatcc tgccacaatt tgccatcaac aacgggcggg ataaacgggc ggaacagctt
      721 acagcgcgca agaatagcct tgcgcgactc aatacccaac tcaacgcact cagcccacag
      781 ccgacgccca ctctcccctt catgaatttt ggcgttaaca tccagtgcag caaacagcgg
      841 atagaaaggg ctggtagaag catggagcat aaaggcgtta ttcaaccgct tatgcgggca
      901 aaaacgcgcc tgtccgcgga tatggttatc ttttttatgg atctgcgacg tctgtgagaa
      961 tcccgcctgc tgtttgtgca ccgactgagt cacaaagatc cccggatcgt tttcgttaag
     1021 ttctaatagc agcggcgagc tatccgccat catcgggata aactgttcgt aaccgaccca
     1081 cgcggaatca aacagaatgt aatcacacag atgcccaacg gtatcgatca cctgacgggc
     1141 gttatagaca gttccgtcat aggttcccag ctgaataatc gccaggcgaa acgggcgcgg
     1201 cagctcggct ttttctggcg caacgtcgcg aatttgctgg cgcagatact cttcattaaa
     1261 acagtgcgca tcaataccgc caatgaaacc aaacgggttg cgtgaagctt ccagatagac
     1321 cggcgtcgcc cccgcctgaa tcagcgcgcc gtgatgattc gacttatggt tgttacggtc
     1381 gaagagcacc agatcgccac gcgttaacag cgcattcgtc accactttat tcgctgccga
     1441 tgtgccgttc agcacaaaat aggttttatc ggcatgaaag actttggctg cgaatttctg
     1501 cgcatctttc gccaataagc agatcgccca attttacgtc agcgttacac atatcggcgc
     1561 gaaagacgtt ctcaccaaag aaatcgtaaa aatggcgtcc ggcaggatgc tttttaaaaa
     1621 acgcaccatg ttgatgtcca gggcaagcaa aggtgctgtt gcccatctca acgtactgcg
     1681 tcagcgtgtc ataaaacggt ggcagcaaat tctcttcata ctgacaggct gcggattcca
     1741 gctccagcca ctgctgctcg ttgccgttga ttaccgccgt aacgcccgca ggtaattcaa
     1801 cagcatgttc ggaatacaaa aacaccggta gatgaaaacc ggtgcgctta agcaacgcaa
     1861 gaatgccact gcgactatcc gcagcggtaa tgacgactgc cacgacgtcc gtaaaatcag
     1921 tatctcccaa cgccaccacg cgacgatgag aagaaagtcg ggataccagt tcactactgg
     1981 cggcaatatt cattgatttc ataagcgcaa acccgtttcg gggaagtaag aataccggac
     2041 aaggtggaaa accctgcccc atgagatatg ggtcaaactg gtcaccagct ccgaccgcca
     2101 gacatcagta aaagcagaaa cgctctgatt ttactgttgt cctgcagtga gcgtgcgtta
     2161 acttcaccgc atgagcagta acatagaaag ggaaacgttt cgcgcgaaac ggcgataagc
     2221 gagagaatgt aaggaggtgg cgtgcatcgg gcaaactccg tacagagagg agaaaattcg
     2281 cgcaatcatg gcacctttcg ctaaagcgtg caagccagga ctttgcgacc agacaagcca
     2341 tcaacaaggc aaaaagttca taataagaaa atcaaacaat acagttaaca ggaactcttg
     2401 tggtcatcgg cccttttatc aacgctagtg cagtcttact gggtggcgtt ctcggcgcac
     2461 tgctcagcca acgcttaccg gaacgtatcc gcatctccat gacatctatt tttggtctgg
     2521 catcgctggg gattggtatt ttactggttg tgaaatgcgc caaccttcca gcgatggttt
     2581 tagctacgct acttggcgct ctaattggcg aaatttgttt gctggagaaa ggtgtcaata
     2641 cagcagtcgc caaagcacaa aatctgtttc gtcactcacg taagaagcca acgcatgaat
     2701 cttttattca gaattatgtc gcgattattg tcctgttttg cgccagcggc accgggatct
     2761 tcggggcgat gaacgaaggg atgaccggcg atccgagtat tttaatcgcc aagtcatttc
     2821 ttgatttctt tacggcgatg atcttcgcct gctcgctggg tattgcggtg tcggtaatta
     2881 gtatcccatt actgatcatc cagttaacgc tggcgtgggc tgccgcgctg atattaccgc
     2941 tgaccacacc gtcgatgatg gcagacttca gcgccgtagg cggtttattg ctgctggcaa
     3001 ccggattacg catctgtggc attaaaatgt tcccggtggt caacatgctt ccagcactcc
     3061 ttctggcaat gccgctttcc gccgcctggt ttgcctgaca atgcgtgcaa tagcggcaaa
     3121 gtgatgatag attgtgcagt ctgcagtaaa ttgaagaaat ttgattgacg agacgaggcg
     3181 aatcaggttt aatgcgcccc gttgcccgga tagctcagtc ggtagagcag gggattgaaa
     3241 atccccgtgt ccttggttcg attccgagtc cgggcaccac taattcttaa gaacccgccc
     3301 acaaggcggg tttttgcttt tggatctgac aataaccttc acgaaaaaag tttgcttata
     3361 aagtctgggg gaattactct cgccacgtta acgagagtaa ttttattgat actaatctcc
     3421 tgatacttta cccccgtcca aactccagcc gctggacatt caccatccca gtcttctcag
     3481 cagcactggc atcaatttgt gtcacccgca gcgcatactt ttcatccagc gcgtttaacc
     3541 agttcagcag gtcattaaac accaccggat ctatccagac ctgaatattc tccccacgct
     3601 cggctatccg cttgatgact accgagtgcg cggaagcact gtcactgata acccgcgata
     3661 cctgcgcagg cgttgttgtg gcggattttc gcgccgcaat aatatccggc gcggcgctcc
     3721 tcagtctggt gttcatcgcc accagttgct gcaacattgt ctcctgttgt actatccgtt
     3781 cgctcaacgg ctgccagatg aggacgtaat atccgacgct aaacaggaac accaccgctg
     3841 ccagtaacat gcccttttca cgcggagaac gccccgccag gtgttgtgcc agccagtgtt
     3901 cgccacggct taactggcgt tcacgccatt gctgaaaata gtgaataaat ttatcgcgta
     3961 acatgttatt tcctccgcaa cgttacgccg ccggaaaccg catcaccctc tttctgtaac
     4021 gcgtcctgtt gcacaacata atcggttgcc agtgcactac gcagtttgtc gaaactggca
     4081 aagttcgcgg cccgtagctg gaggtgaagt atctggcgtt tttgatcaaa ggtaaaacca
     4141 cgcatttcga tgtcggaaag tgacgctgat ttcagggtgc tggctatcgc tgacaactcg
     4201 gcgagcagcc gggtatcgtc ggcctgtggg cgatattttt tcagcgccat cgtcacctga
     4261 gagcgtaaat tcacaatccg cttctgctcc gggaacaacg ttaagaactg ttcctccgcc
     4321 tgggtgcggc tttgcgccac ctgttcgctg acgctccata acgtcacgcc ccgctccact
     4381 gccagcgcaa ctagaatcag caatatcggc agaatcatca cccgccagcg cgcccactgt
     4441 tttcggtagc tgacgcgagg ctgccacggc cctgtcagca ggttcccttc cggttcgcca
     4501 taagtggtaa cggcgggcag agctgtaacg gtcaggcgtt cagcgtctgc gccagcccat
     4561 gctgatagct tttccggtgc aatgccgact acggttagcg aaagcggtaa atcctgctca
     4621 ttgagctggg cgcggaacat gaccggagcc agcgcccgcc cggcgctcca tccccggcat
     4681 tcatcaatgc ggcagataac ccgttgcgca tcgccagcca taaccccaca aggaatggac
     4741 atccagtctg gcgcgacggt agcgcgggtg atgccgtttt cctgcaacca ctgcgcaatg
     4801 ttgcgcatat gctgttggtg aatcactgct acggttgcca gttgctggtc gatattcaac
     4861 ggggcgaaat gcagttcatc gatatcctgg ttcagctctt cttccagtaa ggcgggcaga
     4921 atggtcggta tctgcttacg ggcgacatca ggcagttcaa cctgccagac gctgatccat
     4981 tcgccgggaa tgtagagtcg aatcgcatcg gtttgcagcc attgctggag ctgttcgtta
     5041 gcaacgtcag accagatacc gtgctccaca tcgggtgtac ggcgctgcca atggatggga
     5101 tcagcggcgc aaagcgggaa aaaaatctca aggatggaac tcactcactt tctcctgtct
     5161 gatgccagag aaccgaaaag tgttgtgggc ccatgcggac aattaacgag ttcatcgtca
     5221 gttcaatctc attcacggtg atatctgaac gcagccagaa gtaattgctg tccacgctca
     5281 ggacggtttt tagctgtttt ttagtacgct catcgacgtc agcaagtagc ggctgtgcaa
     5341 gaaactgatc gacatcttcc cagcccttcg ccggacgttg ttgtaataac gcccgcgcct
     5401 gaacggggct taaccacggg tcaaacagcg cctcaagaat cacactttgc gtgacgtcta
     5461 aagtattgat gttgatttgc tggcggatca tcggcagcgc acagaccagc ggtttcagtt
     5521 tttgataaag cccggcgtcc attccctgta ccacgcgcat ctcactgata tcagccagcg
     5581 gttgattggc ggcgtagaac ggcaccgaac gggcgagata ctcgctgtct tcacggccca
     5641 gacgtgtctg cacgctgcgg tcctcgtcaa taaactccca caggctttcg gctatcagtt
     5701 cggcccgata agcaggcaca tccaggcgcg agatcagggc aatcaactgt tgtaccgcaa
     5761 gcggacgcga cgccgttgtc ggctgagcaa gggcattcag gttaaagcaa gcctgtgcgt
     5821 cacgcagggt gaccgcaatt tgcccctgcg gcagagggaa gaaacgaggg ccggatgccc
     5881 acggctgtgc cagatgcacg cgcttttcat ttttcaggct cagactgagc gcgctcagcg
     5941 ccaggctttc cgcactggcg ctgtaccaca gcgcctgctg gtactcctgc tggctgcgcg
     6001 ttcgcccaag ttgttgctgc attcgcccgg aaagcgtgat ggttaccagc atcataaccg
     6061 ccagcaatac cagcaccacg accagtgcca ttccgcgttt tggtggtgag gtgatcatga
     6121 ttattgcggc ccgcgtaaca accagatgcg ttcaatctcg ccccattgcg gcgaatgcag
     6181 ggtcatgcgt accgccacag ggatcgcctg tactgacgac cagctctcct gccagcgcgt
     6241 gccgtcgtag aactgcaaac ggagcgaatc ggccggaatc aacttttgca ttgtcggctt
     6301 cacgctgcct gcggcatcgg tcagcggcca ggctaaccgt tcgagataac cgtcatgaat
     6361 gcggtaaccg acggtgagca gattgctgcg cggcagacgc atcaacgggt taaccacacc
     6421 gccgcgcaca aaacgcattc cttcactctc agacgccagc acgccagcgc cagccagtaa
     6481 agctggttca cgctggccct gatcgcctct taccggacgc ggcatcattt gggtcagatc
     6541 gtgggtcaga aaactcatcg tttgctgcat gaggtttagt ttttgatcgt gatcggcgac
     6601 ggcgctattc acgcgcgtga cgccgttagt cacctgctgc gccatcagtg ccagcgaggc
     6661 aaaaatggca attgccacca acatttccag taacgtgaaa ccagcgcgag tccttctcac
     6721 tgttggcctc ccacggcgct aaaccaggcg cgtcgtgact gaatcaccga cgaaaagtct
     6781 tcgtgacggc tgacttcaat atccaccgcc tggagcagcg cgttgcctgt attcaatggg
     6841 attgttcgcc agtaccagcg gcttcctgcc atatcgctct cgccctgatg cctgaccgcc
     6901 cgctgctcac gggtcatcag gtagagcagc gcagtctggt tttcagctac ccagcccgct
     6961 accgtttttt cctcaagaac gtgctgatta ctcagcgcac cgctggcaat ctgtaacacc
     7021 gccgtggcag ccagcgcaaa aatcgccagc acgagcatca cttccagcaa ggtaaatccg
     7081 cgcttcatgg catcctctca tcacattgat tgagggataa cgcgccatcg tgtgccagct
     7141 ttaccgctca acatgcgttt tgcgccgccg agtagaagcg cagcgtaaac ggcgtggcgg
     7201 gttcaaaagg cgaaaaacgg atctgcggcg tcttcttttt cgcctctttt tgcaactcca
     7261 gttcaatatc gtgcagcgtc aggcgacgac gttgcagctc cagcgcatat tccttctgcc
     7321 agatatcact tccgggttgt aacagcatct gcacctgttt cggcaccgta acctgtgccg
     7381 ataaccgggt cgcagaaacg ggcaaccact gcccctgacg acgctgcata aactgatagc
     7441 caggcgggtc gattagcaca ccgagtgtct gtccttcaat cactgccctg tccttaaact
     7501 gcgcaaagcg agtcagaaaa tcctgtgccg cttttttcgc gggcggttca gaatcggtcg
     7561 caaacgtctg taccacgccc gcactggcaa ggccgataag gaaaatcacc agcatgattt
     7621 ccagaagtgt gaatccgcgt tcaggcactg cttactgaaa ctcctgcaaa ttccagttac
     7681 cgatatctgc gccagcgcct tccccgtttt cctgaccatc cgcccccagg gtataaacat
     7741 caaacagtcc tttttcaccc gggctgagat actgataatc attgccccac ggatcctttg
     7801 gcagtcgttt gatgtatcca ccagtacggt agttgcggga gtccgccata ttggctggct
     7861 gctggatcag cgcttccagc ccctgttctg tggtcggata acgcccgtta tccagtcggt
     7921 acatatctag tgcgttttcc agcgccacga tatcgctgat ggctttttgc cgattggctt
     7981 tctctttgtt gcccaacagg ttaggcacca ccagacttgc caggacgcca agaataacaa
     8041 tcaccaccat cacttccagc agggtaaaac ctgcccgtgg tttgtgtgtg cgggataacg
     8101 aattcatgtg ataactccgt aaattacatt ccaaccatat tattcagttg cagcatcggc
     8161 tcgaggatgg cgatgacaat aaacagcacc acgcccgcca tcatcaccac cagcgcaggc
     8221 tcaaacagcc ccaacgccag ccccacctgg gtatcaaatt cccgttcctg gttaacagcg
     8281 gcctgctcaa gcatggtttc cagttcgccg ctctgttcgc cggaagcgat aatgtacagc
     8341 atcatcggcg ggaacaggcg caactccgcc agcgcggcgc gcagactgct tccttcgcgg
     8401 acgcgatctg ccgccagcag cagttgttgt tcgacatagc gatttgccga cacggcggca
     8461 gcggtctgaa tgccttccag tagcggaaca ctgctggcgg tgaggatgct taatgtgcgg
     8521 gaaaaacgcg ccgtattcag cccgcgcgca acacggcccg tcacgggcag acgcagcaag
     8581 gttttatccc agcgcaggcg catagtagga tttttaagta gccattgccc cagcaccaga
     8641 agcgccagca atcccgccag ccagtaaacg ccgctggcct gtaacgcatc actcatagcg
     8701 ataagcgcac gggtggagga gggtagcgcg tgtccgagat gatcaaactg ttcgatgatt
     8761 ttcggcacca ctgccgtcag caaaatagtg accacgcccg ttgccaccac cagcataacc
     8821 agcggataga gcatggcctg caatagacgt gatttcaggc gctgtcgctg ttcggtgtaa
     8881 tctgccaggc gattgagcac cacgtcgaga tgcccggatt tttctccggc agcgaccatc
     8941 gaacaaaaca gggaatcaaa gacgcgggga tgttcgcgca ggctgtccga cagggtataa
     9001 ccttcctgaa tccggctgcg cagcgccatt ccgaggctct tcacatgcag tttttcactt
     9061 tgctcactga ccgcctgcaa gcaggtttcc aacggcattg ctgcctgcac cagcgttgcc
     9121 agttggcgcg tgaacagcgc cagatctgcc gccgccacgc gacgatgtgc gtgccgccga
     9181 cgctgcaaca ttccccctgc cgatgcattc atcctggctt caatgtgcac ggggataagc
     9241 tctttaccgc gcaacaactg gcgggcatga cgcgcggaat ccgcctcaat catgcctttg
     9301 gttttgcgac cattacgctc cagcgcctga tagtaaaaca gtgccattac gcctccatgg
     9361 ttacccgcag aacttcatcg agagaggttt ctccggcgag cactttctca atgccgttgc
     9421 tgcggatacc cgcagagtgt tgccggacat aacgttccag ctccagctcc ccggcctgac
     9481 ggtggatcaa atcacgcaat gtggcatcca ccacgtcagc tcatggatgg cagtccgtcc
     9541 gcggaaacct ttgtgattac aggcggggca gccctgcgga tggtacagag tgacggttcg
     9601 cgcatcggta atgcccagca ggcgtttttc ttcgtcagtg gcaggcgcgg cctgacggca
     9661 gtcgggacac agcgtgcgga ccagtcgctg cgccatcacg cccgtcagac tggaagagag
     9721 caggaaaggc tccacgccca tatcctgcaa acgcgtgatc gcccccaccg ccgtgttggt
     9781 atgcagcgtg gaaaggacca ggtgtccggt aagcgatgcc tgaacagcga tttctgcggt
     9841 ttcggtatcg cggatttcac cgaccatcac cacatccggg tcctgacgca aaatcgcgcg
     9901 caggccacgg gcgaaggtca tgccgacgcg ggtgttaacc tgtgtctgac cgatcccttc
     9961 aatcatgtat tcgatggggt cttcaaccgt gagaatgtta cgcgaatggt tgttcagctc
    10021 ctgcaatccg gcgtacagcg tggtgctttt gccggaaccc gtcggccccg tcaccagaaa
    10081 gatgccgtgc ggtttgtgta acagctggcg caactgcacg gtcagttgct ggctaagccc
    10141 cagacgctcc agcgtcaggc gggcctggtt tttgtccagc agtcgcagca ccacgcgctc
    10201 gccccaggca gaaggcatgg tggagacacg cacgtcaatc gcccggccgc ccagcagcag
    10261 cgcaatacgt ccatcctgcg gtacgcgctt ttcggcgata tccagccgtg ccatcacctt
    10321 gatacgcgat accagcagcg aggccagttt gcgccccggg cgcagcattt catgtaatgt
    10381 gccgtcaacg cgaaaacgga tcaccagact cttttcaaac gtctcgatgt ggatatccga
    10441 agcgccttct ttaatcgcct ctgccagcat ggcgttgatc agtttgatga tcggcgcatc
    10501 gtcgtcactt tccagcagat cttccgtttc tggcagttct tccgcgagag taaaaaagtc
    10561 ctcagcagaa ccgagatctt ccatcagctg gcgagcctcg gaagagtccc gctgccagac
    10621 cgcattcagc cgctgttcaa attcggcctc gtcgatttgc cgcagcgtaa agggcgcgtt
    10681 cagcccccgc tgcagctcct gcaaaacaga gagcgatagc ggatgaacgt ggaggatctc
    10741 cagcgacgct tcgcaccatg ccaccaggct aaaccgacgg ctgaaactgt agggcagacg
    10801 cacggtcttg gcggtggttt cctgtgctac aggcaccatt aacgcgttct cccggcattg
    10861 aggaacgcgc gaacttccgg cggtaaggcc tggttttgcg ctggcagtac cggctgtgcg
    10921 gtgtgcggca tcaggcttaa accttgctca tcacgataga tctgttcggc gcgcatatag
    10981 ttatatttgc gctgcgacac gccgtctgcc gccataccgt catgcagaat ggtcgggcgg
    11041 ataaacacca tcaggttacg tttttctttt ttatccgccg tcgatttaaa caggttacca
    11101 atcaatggga tatcgcccag cagaggcact ttcgccacgc tttctcccgc ctgatcgtcc
    11161 atcagaccac caagcacaat cagctcacca tcgttagcca gcacggtggt tttcagtttg
    11221 cgctcaccaa acaccacgtc gaggctggtc tgtccttcca ccttcgagac ttcctgctca
    11281 atcaccatct gtaccgcgtt accttcgtta atctgcggcg tgactttcag catgatgccg
    11341 acttttttcc tttctactgt attgaaagga ttgctgttat tggagccaac ggtagagccg
    11401 gttaataccg gaacgtccta gcccaccatg aagaaggctt cctggttgtc cagcgtagtg
    11461 atgctcggcg tggagagaac gttcgagctg gagtcgtttt taaccgcctg caccagtgcc
    11521 atccagtcgc ctttcaccac gccaaccgcc gtaccgctaa atccagaaag aagctgggcg
    11581 agtgtggaga gatcgccgtt ggtatccgga ttaatggtgg tagcgccgtt ttcactgatc
    11641 accgtcgagc ctttctgcgg tttcgcctga gaaatggctg cccccagcgt accgataggg
    11701 atctgcgtac cattagcaaa ctgcattaat ccggcatctt tcgacgccca ctgcacgccg
    11761 aagttgatat tgctaccttc ggcaacttcc acgatcaacg cctcgacgtg cacctgagca
    11821 cggcgaatat ccagttgttc aattacgctt tgcagcgact gcatgatgtc ctgcggcgcg
    11881 gttacaatca gggcattact gtgtttgctg gcggcgatgg atacaacctc acgcccgcta
    11941 ccgaccgtgc cttccgcctc ttctttagcc gccgtgagcg tgccgctgac ctgtttcagc
    12001 acatcgacca gatcttcggc tttgctgtat ttgagataga aaacctggct gttgccgctg
    12061 cgttccattt ctgagtccag ccgacggatc aggcggcgca ttttgtcccg cgtggccggg
    12121 tcaccactga caatcacact gttggtgcgt tcgtcggcga caatttgaga tttcagcgtc
    12181 gcaggctggt tctcgccgct gtttttcgtc aggctttcca gcacgcgggc gatttccgaa
    12241 gccgaggcgt tatccagcgg gatcacctct tcggtgcgat tacccgcgtg atccacgcgc
    12301 tggatcactt ccgtcaggcg ctccacaacg gaggcgcgtc cggtgagcat aatcacgttg
    12361 gagggatcgt aattaacaac gttgcctgag cctgcgctgt cgatcatctg gcgcagaatc
    12421 ggtgccagct cgcgtaccga cacattacgt accggcacta ctttggtgac catttcatcg
    12481 cccgcgtagt tgtcgctgcc ttcaccgacc agcggcagcg gctcgacttt cgcagcgctg
    12541 gatttcacca ccttcagcac gtcgttttcc atcggtacga cggcataccc ctgtgcttcc
    12601 agcaggttaa ggaatagctg gtaatactgg cgttcattga gcggggtcat ggtgcgaata
    12661 ctcactttcc cctgtacgcc cggccccata atgatggttt tattaaggtt agcgccgacg
    12721 gtttcgatga acgatttcag gtcggtatct ttaaaattag cggtgaaagt ggcttcttcc
    12781 gcccagaccg gtgaactgca taatgccgct gccagcacca gcggcagtaa acgctttttt
    12841 gttttgaggc cagttgtctt cttacgccag accgacaacg taatatcacg ccaaaacacg
    12901 atgaatgatt ctcctggatt aaatgcggtt agcgcagcgc gatggaaatg tcgtggcgcg
    12961 cacccttgcg taaaaccgta agttgaatgg aatccattga aggtaactgc cgcatcagag
    13021 caatcattgc tcgtggatca gtgaaatcct gctgatttag cgcaatggcg atatcgcctt
    13081 ccttgaaacc gctggcatcg aacagagaac gatctgcccc cggcttcact gcataaccga
    13141 caattccctc cttacgcaca ggcgtaagct ggatatagtt aaaaattttc tgcggatctt
    13201 tcgccagtgc ctgacgcacg gcagtcggta tctcaactgg cgcactgaca gcaggttcag
    13261 caacagcttg ctttgcttcg tcactgacag cttttttgtt ggtcgcggca acggtggaac
    13321 gctcctcttc agccaggctc aggcgctcta tttttccctg atagcgcagc atcacatggt
    13381 cgcgattgat ttcctcaatc accgcgttgt gcgagccaag cgtttcaccc tgcaaataga
    13441 cctgctgttt accgccttct tcaataaccg cgccgggtct ggcaccaaag gcgatcccgc
    13501 gcgcaccaca ttaagacgcg tttctgccac aggcacaggt tcgggttgtt ttaccggcgt
    13561 ggcgacgggc tgatatttgc caaaccagtt ttgctggctg attaattgca cgtcattttt
    13621 atcgaacgtt tttgcatccg cacggggcgg tttattcgcc gatggggaaa cagccgtata
    13681 ttccgcagaa aaggagaaat agcgccagag tgaatgcgcc acttttgcag aaataataag
    13741 cagcatcagc cagaacatcc cgcgtacaat ttt
//
LOCUS       DAJVUJ010000111        13585 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.111, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000111 DAJVUJ010000000
VERSION     DAJVUJ010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13585
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.111"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(242..1615)
                     /gene="cobA"
                     /locus_tag="OQQ48_002921"
     CDS             complement(242..1615)
                     /gene="cobA"
                     /locus_tag="OQQ48_002921"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCS1953615.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFTTMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            complement(1634..2440)
                     /gene="nirC"
                     /locus_tag="OQQ48_002922"
     CDS             complement(1634..2440)
                     /gene="nirC"
                     /locus_tag="OQQ48_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HCS1953616.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKSNRPVADKFNQTETAAG"
     gene            complement(2567..2893)
                     /gene="nirD"
                     /locus_tag="OQQ48_002923"
     CDS             complement(2567..2893)
                     /gene="nirD"
                     /locus_tag="OQQ48_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HCS1953617.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            complement(2890..5433)
                     /gene="nirB"
                     /locus_tag="OQQ48_002924"
     CDS             complement(2890..5433)
                     /gene="nirB"
                     /locus_tag="OQQ48_002924"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HCS1953618.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRIFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            complement(5695..6876)
                     /gene="tsgA"
                     /locus_tag="OQQ48_002925"
     CDS             complement(5695..6876)
                     /gene="tsgA"
                     /locus_tag="OQQ48_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HCS1953619.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            7147..7719
                     /gene="ppiA"
                     /locus_tag="OQQ48_002926"
                     /gene_synonym="rotA"
     CDS             7147..7719
                     /gene="ppiA"
                     /locus_tag="OQQ48_002926"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HCS1953620.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGAPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            7824..7991
                     /locus_tag="OQQ48_002927"
     CDS             7824..7991
                     /locus_tag="OQQ48_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HCS1953621.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLVELRRHYER"
     gene            7981..8583
                     /locus_tag="OQQ48_002928"
     CDS             7981..8583
                     /locus_tag="OQQ48_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HCS1953622.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKTKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            8615..9178
                     /gene="pabA"
                     /locus_tag="OQQ48_002929"
     CDS             8615..9178
                     /gene="pabA"
                     /locus_tag="OQQ48_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HCS1953623.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGEGVFRGLANPLTVTRYHSLVVEPGSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            9264..10484
                     /gene="argD/dapC"
                     /locus_tag="OQQ48_002930"
     CDS             9264..10484
                     /gene="argD/dapC"
                     /locus_tag="OQQ48_002930"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HCS1953624.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTASTPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGRARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(10551..12641)
                     /locus_tag="OQQ48_002931"
     CDS             complement(10551..12641)
                     /locus_tag="OQQ48_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCS1953625.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(12692..13324)
                     /gene="crp"
                     /locus_tag="OQQ48_002932"
     CDS             complement(12692..13324)
                     /gene="crp"
                     /locus_tag="OQQ48_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HCS1953626.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
BASE COUNT         3289 a         3495 c         3556 g         3245 t
ORIGIN      
        1 ttccctatgt attatatatt aattaatatt cattgattat atgaatataa gcagactgct
       61 cgttaagaac gatacaggaa tatttaattg gcgtcagtga gcgaatgact taattttgta
      121 agtcgctgta acggtgaata taacccatta gcagataata tttattaagg ggttatgttt
      181 tatttgctga atatataaaa agagtcatta agaaggccag aattctctgg cccgggttaa
      241 attaatggtt ggagaaccag ttcagtttat cgcgcaggcc aacaacccga ccaataataa
      301 ttagcgatgg actgttcatt tgctgagcaa gttcaccgag ttgcgtgagc gtaccgtcaa
      361 tcacgcgctg cgtgactgcc gttccgtttt cgacaattgc caccggcatt tcgccaggca
      421 taccgtgttc aatcagcttt tgctgaatag tcgcggcctg attcaacccc atatagaaca
      481 ccagcgtctg tttttctgcc gccaggtttt cccagtccag ctcgccaccg gtttttaagt
      541 gtccggtaat taagcgtacg ctctgggcat aatcgcgatg cgtgagtgga atacccgaat
      601 aggcagagca accagaagct gcggtaatac ccggaaccac cgagaacgga atacccgcgt
      661 tgcacagtgt ttccagctct tcgccaccac ggccaaaaat aaacggatcg ccacctttca
      721 gccgcaccac gcgtttgcct ttttgcgctt cccgcagcag gatctggtta atctcttcct
      781 gcggtacaca gtggtatccc gcgcgtttgc cgacgaaaac gcgatcagca tcgcggcgta
      841 ccagattcat aatatcgtca gaaaccagac ggtcgtagac caccacatct gcctgctgaa
      901 tttgttgcag tcctttcagc gtcagcagcc cggcatcgcc cggacctgca ccaaccagca
      961 ccacttcacc gcgatggtcg agcggttcgt tgattaactg ttcggtcgtt tcagtaatgg
     1021 ctttctgatc gttgtttgcc agcgactgcg ccaggcggtc gttaacgaac aatttctccc
     1081 agaaacggcg acgctcactc atcgtggtga actgttgttt cactcgcccg cgtaattgcc
     1141 cggcatattt cgccacctgg cccagatgca acggcagcag tgattcgagt ttttcgcgca
     1201 acaggcgcgc cagaaccgga gaggtgccgc cagaggagac cgctaccatt agcggtgagc
     1261 ggtcaataat cgacggcata ataaagctgg cggctttcgg cgcatcgact acgttacaga
     1321 agatgcgacg agcttcagcg gcttcgctga cgcgctggtt aagcgtgtca tcatccgtcg
     1381 ctgcaatcgc cagccagcag gtgtcgagaa ggctttcatc aaatggccct tcgacgaggg
     1441 ttaacatgcc tgcatctgcc catgcggtga actgtggaat aaacgctaat gcattcaccg
     1501 ttaagcgagc gcctgcgtct aacagcaacc ttgctttgcg ttccgcgaca tcaccaccgc
     1561 cgacaatcag acagtcgcga tcgcgtaatt ggcaaaatat aggcaaatga tccacgtaaa
     1621 aaccccttag taattaaccg gcagccgttt cagtttgatt aaatttgtcc gcaaccggac
     1681 gattcgattt cggcgtagca taccaataac ccaatcccat gaatacggca cctgataaag
     1741 tattacccag cgtcacccac agcaggttat gaccaatacc cgccagcgtg taggcttcgc
     1801 tgtggttgcc gaaccaggag agcgcgaaca gcgtcatgtt agcgatagag tgctcgtagc
     1861 cggacgcgat aaatgccagc agacaccacc agatagcgat aaatttcgcc gccccttcag
     1921 tgcgcagcgc catccagatt gccaggcaaa ccagccagtt acacaatgca cctttgaaga
     1981 agagtaccat tgccggtgca gtggttttag ccagcgcgac ggagtgaacg atgctggtat
     2041 ctaccggcag caggctaccg ccgccccagc tatagagcat ggcaacgaag acggaaccga
     2101 ccaggttacc cagccaggtt tgcggcagga ttgcccacat ttgcccgtgg ctgatgctgc
     2161 ccgcttttac cccaaaggtg aggaacatgg tgtgtccggt gaacagttca gaaccggcga
     2221 taatcaccag cgttaaggcg ataccaaagg tcgcgcccat caccagcgga cgtacggacg
     2281 ggtcgagcaa attaccgagc gtgaaaatca agatgatccc aagacccaca tacgcgcccg
     2341 ccatggcgga gctgacccaa aagccgagcg ggttatttgc cgacaggcgt gcaatgcgcg
     2401 cagcgttagc cgcacactta ttaatagtgt ctgtaaacat ttgattatcc tttaaaaaat
     2461 aaaaacaaaa aaataaaaca aaaagtttaa aaaatgtcct gcaatatacc gaaggtaatt
     2521 acaggaccat gcaaaaaggg gaggcattgc gcctcccgtt aaaacattaa ccgcgcagct
     2581 gcaccacgcc gtctttcact cgtgcttcgt aatgtttgac ggaaaactgt tcgtcttcca
     2641 tgcacaagcc atcacttaag cgaaaacgct gttttttcag cgggctggcg acccacagct
     2701 cgccctggtg ttccgcaatc agcccgcgtg acagcacgct ggactcgaag aacggatcga
     2761 tgttgctgat cgcaaacacc tggtcgctgt gatacgggcg gaaaatcgcg acctgctcgt
     2821 cgcctaacag cgcgcagacg ccggtttcag gcaggatgtc atcgattttg cagatgtctt
     2881 tccactggct catgcgttgt cctccaccag agttaccggg atacgttcat acggcgttgc
     2941 cggacggtgc tgttcgcgct ctggcaccat ctgcacgttc gggtcacgct tgtcgctgtt
     3001 gatgaagtgt ttgaagcgag tctgcgcgga cggtgtattg accgtttcag tccactcaca
     3061 cactaccgct tcacgcaggc gcgccatctc ttcttccaga tgagcgttca gccccagttt
     3121 gtcgtcaatg atcaccgctt tcaggtaatc gatgccgcct tcgaggtttt ctaaccacgg
     3181 cgcggtacgc gtcagtttgt cggcggtacg gatgtagaac atcatgaagc ggtcgagata
     3241 tttgatcagc gtttcgcgat cgatatcagc tgccagcaga tccgcatgac gcggtttcat
     3301 gccgccgttg ccgcaaacat acaggttcca gcctttttca gtggcgataa tacccacgtc
     3361 tttaccctga gcttctgaac attcacgggt acagccggag acaccgaact tcattttgtg
     3421 cggcgtacgg atgcctttgt agcggttttc cagttccacg ccgaggccga cgctgtcgcc
     3481 aacgccgtag cggcaccagg tgctacccac gcaggttttc gccatacgca gtgctttcgc
     3541 ataggcatga ccggtttcga agccggcttc aatcagctga cgccagatct ctggcagatc
     3601 gtctttctgt gcgccaaaca tcgccaggcg ctgggaaccg gtgattttgg tgtagagatt
     3661 aaattcacgc gcgatacgac ccactgccat cagcccttcc ggggtgattt caccgcccgg
     3721 agaacgcggg atcaccgagt aggtcccgtc tttctggatg ttagcgagga agttgtcgtt
     3781 agaatcttgc agcggagtat gttctggctt cagaatgtat tcgttccagc aggaggccag
     3841 cagcgaaccg acggtcggtt tacaaacttc acaaccgtag cctttgccgt gtttcgccag
     3901 cagttcttcg aaggttttaa tgccttcaac gcggatcaaa tggaacagtt cctgacgcga
     3961 ataagcaaag tgttcgcaca ggttgttgtt aacttcgatg ccctgtttcg ccagttccgc
     4021 gttcagtacc tgagtgacca gcgggataca gccaccgcag ccagtacccg ctttggtttc
     4081 agctttcagc gccgcaactg tgtggcagcc tttgttgatg gcagcaatca gatcaccttt
     4141 agtgacgtcg aagcaggagc agatttgcgc gctgtccggc agtttatcaa caccgataga
     4201 cggcttaccg ctacccgagt gcgccggcag aatcagagaa tccgggtttt ccggcagctc
     4261 gatagcattc agcaccagtt gcagcaagtt accgtagtcg ctggtatcgc ccaccagtac
     4321 cgcaccgagc agggttttgt tgtcttcgct gacgatcagg cgtttgtaga tctctttgct
     4381 ttcgtcgagg taaacgtagc tacgtgcgcc aggcgtgcga ccatgagcat caccaatacc
     4441 gcctacgtcc acgcccagca gtttcagctt ggcgctaagg tcagcacctt caaaggcgtt
     4501 ttcgctaccg agaatatggt caacggcgac ctgcgccatt ttgtagccag gtgctaccag
     4561 accaaatata cggttgttcc agcttgcgca ttcaccgata gcgtagatat ccggatcgga
     4621 agtttggcag gaatcattaa tgacgatacc cccacgcgga gcaacgtcca gaccacactg
     4681 ggttgccagc ttatcgcgcg gacggatacc ggtagagaag acgataaagt cgacttccag
     4741 ttcgctgccg tcggcaaaac gcatggtttt acgtgcttcg acaccttcct gcacaatctc
     4801 aagggtgttt ttgctggtgt gaacgcgcac gcccatactt tcgattttgc gacgcagctg
     4861 ctcgccaccc atctgatcaa gctgttccgc catcagcata ggggcaaatt cgataacgtg
     4921 ggtttcaata cctaagtttt tcagcgcgcc agcggcttcc agacctaaca ggccgccacc
     4981 aacaacggca ccgcgtttgc tgcgacgggc gcaggattca atggcgttga ggtcttcaat
     5041 ggtgcgatag acaaagcagt cctgagtatc agaacctttg attggtggga tccacgggta
     5101 ggaaccggtt gccatgatca gcttgtcata aaaaacggta cgtccggcgc tggagtgaat
     5161 caccttctcc tgacggttga tggtgatagc gcgttcgccg accagaactt tgatgccgtg
     5221 tttctcgtag aagccttcgc gcaccagcga cagctcttcg gcggtgtggt gagagaagta
     5281 agacgagagg tgtacgcggt cataagcgat gcgcggttct tcacagaaaa cggtaatatc
     5341 aaagttggcc gcatcagatt tatcaagaag atcttcgata aagcgatggc cgaccatacc
     5401 gttaccgata attgcgagtc tgactttgct catttttgcc tcgatttctt ttctattacc
     5461 gcctacctta acgattcagc aaccccgctt attgatgtaa atcaaattca cctttatata
     5521 ccccttaagg ggtatattgc tgatttgtat aaattttctt aagtcacgga attgtctgac
     5581 atttcatgtt tgtgcaaaaa atagacaaaa tgaacgggtt tatagaaata gatgcacgta
     5641 gcggggttag agaggaaaag gccggacgac gatagtgccg tccggcatta gcaattaatg
     5701 agaggtcagg gtgttatgct gacggtgacg gctgacgaaa cctaacagga agcacatcac
     5761 aaagacgaca gcgtacagac cgtttgctgt aagcagtgcc gcctgcggac cgctatgttc
     5821 aacaatcggg ccggtaacca caaaggtcaa catagtaccg atggttccgc aggtcaggac
     5881 aaagttaacc agttttggcg acggtacttt ggtctgctgt gaacccaggg tgatgatggt
     5941 ggtatagatc gcgctggaga agaagcccag agcgagaatt gaccacgcca tatgtgctgg
     6001 tgttccggtg ttaaagacgt acatcagaat cgcagccaga ccagccagta cggtcagaat
     6061 gcgttgcaaa tcaaagaagc gaagaataaa gctgaacgcc cacatgccga ccatgtatga
     6121 catccagaag ttactcacca gcgtgcccgc gtcgttcagg ctcatgccca ggcctttggc
     6181 atactcaggc acccaggaga taaaacctaa ctgaccgagg atgtagcaca gcgccgcaac
     6241 agagagaaac agtacgccga tcccccactt ttctttctct accggagcat ccgtttttgg
     6301 tgcatgtttg cccagtgctg ggaactcaca gccgaaggtc agaataaaaa tagcgacata
     6361 caccagcccg atgcaggcat aaacccagta ccactcaatg ctgcgcgcca gtagaaacgc
     6421 ggcgatcatt gggaaaatca tcccagccat actgaagaag gagtcagtaa ataacaggcg
     6481 ggaaccgcgc tgacgccctt catacatttg tgttatcagg aatgtaccaa tcgacatagt
     6541 aatgccgctg accaccccga gaatgaacat cgccgtcgag aacagcgcca ggctgtggct
     6601 gaacatcaaa ccggcaaccg ccagcaccat caggagaaag ccaaaacgta actgcgtttt
     6661 caacgggacg atttccatca gccaggcgtt gaggaagata gagattaaaa tgccggcgtt
     6721 gaggaaggtg aaggtgttac tcatactgga aacaggcaga ttgaaataat cggcgatatt
     6781 tcccatcacc atcccggtga caataaccaa cgcaccagtc agtgcgtagg agagaaagct
     6841 aatccatgtg agcttgatgc gattgctgtt agtcatgatt ggcctgcgtt caaaaataaa
     6901 atggcatagc gggatatgcc gcgagcgggt gattttaggt gattttgtga tctgtttaaa
     6961 tgttttattg caatcggttg ctaaattgca ttttaagaag tgatcttgat cacggaataa
     7021 aaagtgatcg tcaggttaca tatatttcag atacgtaaaa ttaggtaaag ggatggcctt
     7081 gttcttgaag gctatttaga atctcttcac ttgctttttt tctgctctgt ttgttaagga
     7141 aatctcatgt tcaaatcgac cctggcggcg atggctgctg ttttcgctct ttctgctctt
     7201 tctcccgcag caatggcagc gaaaggggcc ccgcacgtat tgttgacaac ctcagctggt
     7261 aacatcgaac tggagctgga taaacaaaaa gcgccagtgt ctgtgcaaaa ctttgtcgat
     7321 tatgtgaaca gcggttttta taacaacact acctttcacc gcgtcattcc tggctttatg
     7381 attcagggcg gcggtttcac cgagcagatg cagcagaaaa aaccaaaccc gccaatcaaa
     7441 aatgaagccg ataacggcct gcgcaacacg cgtggcacca tcgcgatggc gcgtaccgct
     7501 gacaaagata gcgccaccag ccagttcttt atcaacgttg ccgataacgc cttccttgac
     7561 catggtcagc gtgatttcgg ttacgcggta tttggtaaag tggtgaaagg catggacgtg
     7621 gccgataaga tttcccaggt gccgactcat gacgttggtc cgtaccagaa tgtgccgtca
     7681 aaaccggtag ttatcctttc cgctaaagtc ctgccgtaat gatttctcgc gcgggcaatc
     7741 ttgcccgcgc ttctgctctc ccggcgtaac ccggatttgc cgcttatact tgtggcaaat
     7801 ggacacgttc agggaggcat caagtgaaga aactcaccga taagcaaaag tcccgtctct
     7861 gggagcttca gcgtaatcgt aatttccagg ccagtcgccg tcttgaaggc gtcgagatgc
     7921 ctttagtcac tcttactgcc gcagaggctt tagcgcgcct tgtagagctg aggaggcact
     7981 atgagcgata aattcggcga agggcgcgat ccgtatcttt atccaggcct tgatatcatg
     8041 cgtaaccggc tgaacatccg ccagcaacaa cggctggaac aggccgctta cgaaatgacg
     8101 gcgctgcgtg ctgcgaccat tgagcttggt ccgctggtgc gcggtttacc gcatttgcga
     8161 actatccatc gccagctgta tcaggatatt ttcgactggg cagggcaact gcgtgaagtt
     8221 gatatttatc agggcgatac gccgttctgc cactttgctt atatagaaaa agagggcaat
     8281 gccctgatgc aggatctgga ggaagaaggt tatctggttg gcctggagaa aacgaagttc
     8341 gtcgagcggc tggcgcatta ctattgtgaa atcaacgtgc tgcatccctt ccgggtggga
     8401 agtggtctgg cacagcggat cttcttcgag caactggcga ttcatgccgg atatcaactg
     8461 agctggcagg gtatcgaaaa agaggcctgg aatcaggcaa atcagagtgg ggcaatgggg
     8521 gatctcaccg cactgcagat gatatttagc aaagtggtaa gcgaagccgg ggaatctgag
     8581 taaaatagcg cggttctttc gtaccggagc cgccatgatc ctgcttattg ataactacga
     8641 ttcttttacc tggaacctct accagtactt ttgtgaactg ggggcggatg tgctggttaa
     8701 gcgcaacgat gcgttgacgt tggcggatat cgacgccctt aaaccacaaa aaattgtcat
     8761 ctcacccggc ccctgtacgc cagatgaagc cgggatctcc cttgacgtta ttcgccacta
     8821 tgccgggcgc ttgccgattc ttggcgtctg cctcggtcat caggcaatgg cgcaggcatt
     8881 tggcggtaaa gttgtgcgcg ccgcaaaggt catgcacggc aaaacctcgc cgattacaca
     8941 taacggtgag ggcgtatttc gggggctggc aaatccactt accgtgacac gctaccattc
     9001 gctggtggtg gaacctggct cattaccagc gtgctttgac gtgacggcct ggagcgaaac
     9061 ccgcgagatt atggggattc gccatcgcca gtgggatctg gaaggtgtgc agttccatcc
     9121 agaaagtatt cttagcgaac aaggacatca actgctggct aatttcctgc atctctgatt
     9181 tccgattgcc attcagtgat tttttatgca tattttgtga ttataatttc atatttattt
     9241 atgcgtaaca gggtgatcat gagatggcaa ttgaacaaac agcaattaca cgcgcgactt
     9301 tcgatgaagt gatcctgccg atttatgctc cggcagagtt tattccggta aaaggtcagg
     9361 gcagccgaat ctgggatcag caaggcaagg agtatgtcga tttcgcgggt ggcattgcag
     9421 ttacggcgtt gggccattgc catcctgcgc tggtgaacgc gttaaaaacc cagggcgaaa
     9481 ctctgtggca tatcagtaac gttttcacca atgaaccggc gctgcgtctt gggcgcaaac
     9541 tgattgaggc gacgtttgcc gaacgcgtgg tgtttatgaa ctccggcacg gaagctaacg
     9601 aaaccgcctt taaactggca cgccattacg cctgtgtgcg tcatagcccg ttcaaaacca
     9661 aaattattgc cttccataac gcttttcatg gtcgctcgct gtttaccgtt tcggtgggtg
     9721 ggcagccaaa atattccgac ggctttgggc cgaaaccggc agacatcatc cacgttccct
     9781 ttaacgatct ccatgcagtg aaagcggtga tggatgatca cacctgtgcg gtggtggttg
     9841 agccgatcca gggcgagggc ggtgtgacgg catcgacgcc agagtttttg cagggcttgc
     9901 gcgagctgtg cgatcaacat caggcattat tggtgtttga tgaagtgcag tgcgggatgg
     9961 ggcggactgg cgatttgttt gcttacatgt actacggtgt gacgccggat attctgactt
    10021 ctgcgaaagc gttaggcggc ggcttcccga ttagcgccat gctgaccacg gcggaaattg
    10081 cttctgcgtt tcatcctggt tctcacggtt ccacctacgg cggtaatcct ctggcctgtg
    10141 cagtagcggg cgcggcgttt gatatcatca atacccctga agtgctggaa ggcattcagg
    10201 cgaaacgcca gcgttttgtt gaccatttgc agaagatcga tcagcagtac gatgtgttta
    10261 gcgatattcg cggtatgggg ctgttaattg gcgcggagct gaaaccacac tacaaaggtc
    10321 gggcgcgtga tttcctgtat gcgggtgcag aggttggcgt aatggtgctg aatgccggac
    10381 cggatgtgat gcgtttagca ccgtcgctgg tggtggaaga tgcggatatc gatgaaggga
    10441 tgcaacgttt cgcccacgcg gtggcgaagg tggttggggc gtaaataaga cgcatcaggc
    10501 aaaatatgtc ggatgcggcg tgaatgcctt atccgatggt ttagcctgta ttacgccttc
    10561 gaatcccgca acttgcgact cagccaaatc ccgtgatgcg gtcgctgacg ccatgccatc
    10621 gacgaaatgg tgtgcatggt gttcagatga ccaataaccc gctgtaaatg ctgctccagc
    10681 gtacctgccg cgccttcgtg cggctgcatc tccggcgcat ccatgatatt ggcatcgcca
    10741 gaactacccg gttcgtcata ctccagtcgc tgctgacaac gctgaatggc gatttcacaa
    10801 gactgtaaat actcttgtgc cagttcaggt ggcaatgccc ggtgttcccg cgccagcgtg
    10861 gtcatggcat taatatgctc aacaataaac tggctgtgcg ttacccacag tttcatatct
    10921 gccagataat ggctgttaaa cgccggttcc tgcatcgcct gattcaatga gttatacaga
    10981 gtgttatgtg cctgatttac ccgcattcgc tgccaggcca gtggcgtagg ttgcggatcc
    11041 tcgctaagaa tcaagcgaat cgcttcctga taggcttcta aagcgtcatg ggcgttttta
    11101 cgcaataacc cgctctgcca ctgcggccac agccagacag taccgccgaa agcaattaaa
    11161 caaccaataa tggtatcgat aagacgcgga aggatgtatt gctcgccgtt caaccacaat
    11221 agttgcaggg tatacactgc ggtaatagta aaaccgaccg tcgcccagcc gtagtttttg
    11281 cgcaatatca ggtagctggc gagggtggta atcagcatca acgtcagggt gtaaccttcg
    11341 ggaattttaa agtgcagcgc cacgcccgca atgattaacc cgaccacggt tcccacggag
    11401 cgattcacaa tcctcagacg ggttgcgcca tagccatttt gtgtcaccag caataccgtc
    11461 atcaggatcc agtacgactt cggcagatgc agcgcggtgc ccatcaggct ggcaacgctt
    11521 aacatcacac tgagtcgtcc ggcattgcgt agcgccggag actttagtga cagataactt
    11581 ttcagcgcag gaagtaatgg cattcgccgc tgtttatcgg ccagtaagtc acgggcatag
    11641 agcggttttt gggtgcgcag cacgcgggcg atgcggctga aatgccagta gcagaattgc
    11701 ccaaccggat tatccggatg ctggcgggcg attttttcca gtgcgccaat ttgcttttcc
    11761 atcgtaaaac gcgttggcag gcgatggtaa agaatgtcat cagccagcac gcgcaggcga
    11821 gcggcgacgg tttgcgcatt ccagcggata acttcttccg catggctacg ctcgaccagc
    11881 ttttgcacct cttccggctg atgcaaactg accgaaatat gttcctgtaa atccagcgcc
    11941 tcctggaaaa tacgcagcat ccgcttgtag tcagtattat tttgcgcgga aagcatatgc
    12001 atttgctgat agcactgggt aattagatcg accgcttttt gctggcgcac cagcagcggc
    12061 ggcagcgctt tttcagggtc ggtgtgctgg gtaagcaggc tgtatttggc ttcacaataa
    12121 tctgccagtt cacggtacag cagacttagt gactcgcgca gcggttgttc gcgccagatc
    12181 cagaaccaaa accagttaaa caatccgtac cagagagtgc ccaacgcata gatgagcaac
    12241 ggttcccaga ccggcatgta tcccgccaga ctgagggtaa aaatggctgc cagcagcgat
    12301 gcgggaagca attttgcgtg caatggcccc aactcagcag tgacgccaag taccagcgtt
    12361 aatccggtca gcaaaaaggg caggggaaca tcttttgcca gtagcagctg tgtcagtaag
    12421 ctacaggtgg caaacagcga cgcaccaatg attaagcgtt tgaaaaaacg tttatgaggc
    12481 gtatcaaggc ccgcaatatt gcaacaggca ggaacgagag agaagagcag accgaatcgt
    12541 aattcgccaa gcattaaccc aacggccacg ggcaaacata gcaccagcgt ttgtcgaagt
    12601 gcatagttga tatcggggtg ataaatcagt ctgcgccaca tcgggggaaa caaaatggcg
    12661 cgctaccagg taacgcgcca ctccgacggg attaacgagt gccgtaaacg acgatggttt
    12721 taccgtgtgc ggagatcagg ttctgatctt caagcatctt cagaatgcgt cccacggttt
    12781 cacgagaaca gccgacaatc tgaccgattt cctgacgggt aattttgatt tgcataccgt
    12841 ccgggtgagt catagcgtct ggttgttttg ccagattcag cagagtctgt gcaatgcggc
    12901 ccgtcacgtc gaggaacgcc aggttgccca ctttctctga agtgacttgc agacgacgcg
    12961 ccatctgtgc agacagacgc atcagaatgt ccgggtttac ctgaatcaat tggcgaaatt
    13021 ttttgtacga aatttcagcc acttcacagg cggttttcgc acgtacccat gcgctacgtt
    13081 cctggccctc ttcaaacagg cccagttcgc caataaaatc accctgattc agataggaga
    13141 ggatcatttc tttaccctct tcgtctttga tcagcactgc cacagagcct ttaacgatgt
    13201 agtacagcgt ttccgctttt tcaccctggt gaataagcgt gctcttggat gggtacttat
    13261 gaatgtggca atgagacaag aaccattcga gagtcgggtc tgtttgcggt ttgccaagca
    13321 ccatgcgcgg ttatcctctg ttataagctt tctccagagc cagataacgc cgctgtctct
    13381 ggattgccga aatatgcttc ccgctacctg ggaaggggct atcaactgta ctgcacggta
    13441 atgtgacgtc ctttgcatac atgcagtaca tcaatgtatt actgtagcat cctgactgtt
    13501 ttagcatagc tttcgctttg tgtctcctgg tgtctcgctt cagcatgacc caggtcgcct
    13561 tccgttgcgc gatttggtta gtacg
//
LOCUS       DAJVUJ010000112        13523 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.112, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000112 DAJVUJ010000000
VERSION     DAJVUJ010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13523
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.112"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..187
                     /locus_tag="OQQ48_002933"
     CDS             <1..187
                     /locus_tag="OQQ48_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCS1953627.1"
                     /translation="CWSLAAGANPSFIASQMGHTNAQMVFNVYGAWMKDNNHEQIELL
                     NRRLSESVPCMPHKKAG"
     gene            208..909
                     /gene="mlrA"
                     /locus_tag="OQQ48_002934"
     CDS             208..909
                     /gene="mlrA"
                     /locus_tag="OQQ48_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HCS1953628.1"
                     /translation="MGLPLIPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIK
                     RWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRTWIKERGQDYP
                     AQTLTTHLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARRKQGKDALVVGW
                     NIQDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGENRTSAQQQQL
                     TSWQEQGHDIFPLGI"
     gene            969..1076
                     /locus_tag="OQQ48_002935"
     CDS             969..1076
                     /locus_tag="OQQ48_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HCS1953629.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(1057..1788)
                     /gene="yehW"
                     /locus_tag="OQQ48_002936"
     CDS             complement(1057..1788)
                     /gene="yehW"
                     /locus_tag="OQQ48_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HCS1953630.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFVVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(1793..2719)
                     /gene="yehX"
                     /locus_tag="OQQ48_002937"
     CDS             complement(1793..2719)
                     /gene="yehX"
                     /locus_tag="OQQ48_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HCS1953631.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVCREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLEEA"
     gene            complement(2712..3869)
                     /gene="yehY"
                     /locus_tag="OQQ48_002938"
     CDS             complement(2712..3869)
                     /gene="yehY"
                     /locus_tag="OQQ48_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HCS1953632.1"
                     /translation="MTYFRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPGKKGSICALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            complement(3876..4793)
                     /gene="osmF"
                     /locus_tag="OQQ48_002939"
     CDS             complement(3876..4793)
                     /gene="osmF"
                     /locus_tag="OQQ48_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HCS1953633.1"
                     /translation="MPLSKVWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDSEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLKEGGTF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQISGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            complement(5045..7342)
                     /gene="bglX"
                     /locus_tag="OQQ48_002940"
     CDS             complement(5045..7342)
                     /gene="bglX"
                     /locus_tag="OQQ48_002940"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HCS1953634.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPFTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDMVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            7538..9253
                     /gene="dld"
                     /locus_tag="OQQ48_002941"
     CDS             7538..9253
                     /gene="dld"
                     /locus_tag="OQQ48_002941"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCS1953635.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPSSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALQRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(9292..10224)
                     /gene="pbpG"
                     /locus_tag="OQQ48_002942"
     CDS             complement(9292..10224)
                     /gene="pbpG"
                     /locus_tag="OQQ48_002942"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HCS1953636.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAAITASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(10398..10985)
                     /locus_tag="OQQ48_002943"
     CDS             complement(10398..10985)
                     /locus_tag="OQQ48_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HCS1953637.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            11155..11733
                     /locus_tag="OQQ48_002944"
     CDS             11155..11733
                     /locus_tag="OQQ48_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCS1953638.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDHQA"
     gene            complement(11863..12624)
                     /locus_tag="OQQ48_002945"
     CDS             complement(11863..12624)
                     /locus_tag="OQQ48_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCS1953639.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKAMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(12677..>13523)
                     /gene="mdtQ"
                     /locus_tag="OQQ48_002946"
     CDS             complement(12677..>13523)
                     /gene="mdtQ"
                     /locus_tag="OQQ48_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HCS1953640.1"
                     /translation="QAALNTVLQQIEKEQNTIIATDRQLYQNGITSSVEGVETDINAS
                     KTRQQLNDVAGKMKIIEARLSALTNNQTKSLKLKPVALPKVASQLPDELGYSLLARRA
                     DLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQDALHLSDLFRHSAQQMGVTAGLT
                     LPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAVNDVARAASQVQTLAEKNQHQAQI
                     ERDTLRVVGLAQARFNAGIIAGSRVSEARIPALRERANGLLLQGQWLDASIQLTGALG
                     GGYKR"
BASE COUNT         3240 a         3779 c         3416 g         3088 t
ORIGIN      
        1 atgctggtca cttgccgcag gagctaatcc tagttttatc gcaagccaga tgggccacac
       61 aaacgcacaa atggtattca atgtttacgg agcatggatg aaagacaaca atcacgaaca
      121 gatagaactt cttaacagaa gactatctga aagtgtccca tgcatgcccc ataagaaagc
      181 ggggtaaaat aaaaacttgc aaaatcaatt ggtttacctt taatccctgt cacgttacgc
      241 gcgtggcaga ggcgttacgg gttgctgaaa ccgcaacgga cagacggcgg tcatcgactg
      301 ttcaacgatg ccgatattga ccggatccgc gagatcaaac gctggatcga caacggcgtg
      361 caggtcagca aagttaaaat gctgctcagt aatgaaaatg ttgatgtgca gaacggctgg
      421 cgcgatcagc aagaaacatt actgacctac ctgcaaagcg gcaatctgca tagcctgcga
      481 acgtggatca aagagcgcgg tcaggattac cccgcccaga cactcaccac acatctgttt
      541 attcctctgc gccgacggct tcagtgccaa caaccgactc tccaggcgct gctggcgatc
      601 ctcgacggcg tactgatcaa ctacatcgcc atttgtctgg cttcggcacg tagaaaacag
      661 ggtaaagatg cgctggtggt tggctggaat attcaggata ccacccgtct gtggctggag
      721 ggctggattg ccagtcaaca aggatggcgc attgatgtcc tcgcccactc gctcaatcaa
      781 ctacgccctg aactattcga aggccgtaca ttgctggtgt ggtgcggtga aaatcgaacc
      841 tccgcccaac agcagcaact caccagttgg caagaacaag gccatgatat tttcccactc
      901 ggcatttaat gattcgttaa caaatgcgct ttactgtaca atcctttcgt taacataagg
      961 agtgcaatat gcgcatagct aaaattgggg tcatcgccct gttcctgttt atggcgttag
     1021 gcggaattgg tggcgtcatg ctcgcaggtt atacctttat tttgcgtgct ggctaagcgc
     1081 ctgcaccagc ctttcaaaca ggcggtctgc aatgatcgcc gccagtgcca ccagtaacgc
     1141 cccctggatc acatacgcgg tattaaatcc gctaagcccg atgatgatgg gcgtacccag
     1201 cgtgctggcc cctaccgttg aggcgatcgt cgccgtacca atgttgataa tcaccgaagt
     1261 tcgcacgccc gccagaatca ccggagccgc cagcggtagc tcgaccttac gcagtcgctg
     1321 accacgactc attcccatac ctttcgcaac ttctgtcacg ctggcatcaa tcgctcccag
     1381 cccggcaagt gtcgcctgca ggacgggcag cacaccgtaa aggatcaagg cgataatcgc
     1441 tggttgcaga ccaaagccga tcaccggaac ggcaatcgcc agcactgcga caggcggaaa
     1501 agtctgtcca acggcggcaa tagtttccac cagtgggcga aattccgcgc cccacgggcg
     1561 agtgacagca attccggcac cagtgccaat gatcaccaca aacaaactcg aaattcccac
     1621 cagccagaaa tgagccagtg ccagagctgc aaaactttct tgctgataaa cgggtcgtgg
     1681 cagttgtggg aacaaggcag caaacagcgg ctggctgtaa ggcagccaga aaatcagtgc
     1741 cacaaacaaa gcaatgagcc agaacagcgg atcgcgcaac atcttcatac gcttacgcct
     1801 cctccagcag atcctgaaaa tgcagcgtgc cgcaaggctg gccctgcgtg ttcaccaccg
     1861 gcagcacctc gcatccccgc gcgacaaaca gggagagcgc atcgcgtagc gtcatctctt
     1921 ctgccagtgc ctcaccttct gcccgttctt cgcgacacac gtaatccgcc acactacgta
     1981 acgaaagcag gcgcacaccc agttcgctgc gcccaaaaaa ctggcggaca aaatcgtttg
     2041 ccggacgagt cagcatcgtc agcggattgc cctgctgcac cacttcaccg tgatccatca
     2101 ataccagatg ttctgccagc cgtagcgcct catcaatatc atgagtgacc agcacaatgg
     2161 tacgccccag caaacggtga atgcgcgtca tctcttgttg caacgcgccg cgcgttaccg
     2221 ggtccagtgc gccaaaaggt tcatccatca gtaagacttg cggatcggca gccagtgcgc
     2281 gcgccactcc cacacgttgc tgctgaccac cggaaagctg atgcggataa cgctcacgta
     2341 aatttgactc cagccccagt agcgccatta attcgtcgat acgatcgtca atccgcgccc
     2401 gcgaccattt ttgtaattgc ggcacggtag cgatgttttg cgccacgctc cagtggggga
     2461 acaggccaat agattgaatg gcatagccca tccggcggcg caactccagt actggcagcg
     2521 agcgaatttc ttctccggca aagcggatca ctccgctgtc atgctccacc aggcggttaa
     2581 tcattttcag ggtggtggat ttgccggagc cagatgtgcc aatcagcacc gaaaaactcc
     2641 cttcctgaaa attgagattg agatcgttaa cggctttttg tgcgccgaac agtttgctga
     2701 catggctaaa ttcaatcatt acgtttcacc ttcagcagtg cgataagcaa atcgaacagc
     2761 gcgtcggtaa gcaccgccag aacaattacc gggatcaccc ccagcaacac taaatcaatg
     2821 gcgctgctta gcagcccctg gaaaaccagc gcaccaaaac cgcctgcgcc gattaacgcc
     2881 gcaatcaccg ccatacctac agtttgcacc atcaccaccc gcaggctgcg cagaaatacc
     2941 ggtaacgcca gtggtaactg aacatgcagg aatcgctgcg ccccgctcat gcccatcgct
     3001 ctggcactct ccagcacatc gcgcgggatc tggttcaaac cgactaccac gccgcgcacc
     3061 agcggcagca aggcatagag caccagcgca atcagtgcgg gtgtcattcc ggttcctgct
     3121 atgccgagct tccccagcca cggaaaggcc gtcaccagcg cggcaagcgg cgcaatcaac
     3181 aggccaaaga gcgccaccga aggcacggtc tgaatgacat tgagcagaga aaaaattgcc
     3241 ccctgccgag cagtggaaaa gtagcaccag atgcccaacg gcacaccaat cactaacgca
     3301 ggcagcaccg caccaaacag caacgtcaga tgttgtgcca gcgcgtcgtc aaacacatcc
     3361 tgacggttgg cgtattcttt cattagtgag agatcgttaa gcgtgccgga gtacagcaac
     3421 cacagcggaa taatggcaat ctgcatatgc aacaaccagc gccacagcgg atgcgtggag
     3481 attcggcgga tggcatcgct acaggccagc aatgccagcg ccgcagccag ccagaaacca
     3541 ctgccgaggc tggtacgcgc cagcgcactg ccattttgcg ccagttgtgt cgccgccttt
     3601 cccgcgcccc acaccagcaa tacgaagacg aattgcgcca gaatgagtgc acaaatgctg
     3661 ccttttttac cgggaataaa acaggccgtc agccaggcgc aaccaacgcc caccagcatc
     3721 cagatcgttt gcggccacag ctgccagaga tgacgcccct cacccgaaac taaacgatta
     3781 ggcgcgtaac tgataaacgg cagcgccgct gcgattgccg tcagcaacag cagcagcgcc
     3841 agaacaggat taatacggaa ataagtcacg ggaaattact tcgtccaccc tttttgtttc
     3901 aggtagtcgg cagccacttt tttggcatcc agtccttcca cagcaatgct ggcattcagt
     3961 tgctgcaatg tttttgcatc gaggctggcg aagactggct gtagccactg tgccatttgc
     4021 ggatactcct tcaacaccga ctcacgcacc actggtgcag gcgcgtagat aggttgcaca
     4081 ccttgcggat cgcttaaggt ttgcagcccc agcgccgcta ccgggccgtc agtgccgtaa
     4141 gccattgcag cattaacgcc agagatttgc tgggcagcgg ctttgatcgt caccgccgtg
     4201 tcgccaccag ccagtgacag caactgatcc tgaccgagct taaagccata ggctttttca
     4261 aacgcgggta acgcatcggc gcgttcgata aactctgccg aggctgccag tttgaaggtg
     4321 ccgccctctt tcagataacg actcaggtcg gcaagcgaag tgagtttatt tttctctgcc
     4381 acatcctgac gcacggcgat agtccaggtg ttatttgcag gcgcgggcgt cagccagatt
     4441 aacttgttgt gctccgaatc gagctttttg actttctcgt aaccttgctg cgcgtttttc
     4501 cacgctgcat cgttttcatc tttaaagaag aaagcgccat tgccggtata ttccggatag
     4561 atatccagtt caccggaagt aatcgccccg cgcaccacag gagtcgttcc gagttgcact
     4621 ttattcaccg tcggtacgcc gtggctttcc agcacctgca agatgatgtt gccgagtagc
     4681 gcgccttcgg tatcgatttt tgaaccgact ttaacggggg aagccgcctg tagcggcagg
     4741 ctcacggctg ccaacataac cagtgaacct gcccagacct ttgagagtgg catgatgctt
     4801 tcctcattct tttactgttg ttttcagcga attaagagaa aagcatagtt gataatgaca
     4861 gagttagcct gagcggaagg attcagttgc agaatcagat aaatacttaa agggcatatt
     4921 ttgcccggcg tttcttgccg gatgcggcgt aaacgcctta tccggcctac aaaggtcgaa
     4981 aattcagcaa gttaagatat gaacatcagg caaagggcgc aaacgtcgcg cccttactaa
     5041 agcattacag caactcaaac tcgcctttct taacgcgtgc ggaatcagtg ccgataaaga
     5101 cattgaactt gccaggctcg gcgtcatatt tcatctgttg attccagaac ttcagcgcct
     5161 cgatatcaat cgggaagctg acagtctgag tttcgcccgg tttcagggtg attttctcaa
     5221 agcctttcag ctgcttcact ggacggctca tggaagccgt cacatcctgc aagtacatct
     5281 gtactaccgt ggccccttcg cgtttaccgg tgttcgtcac ctgcacgctg gccgtcactt
     5341 tgccgtcacg cttcatggtc ggcgcagaaa gtttcacatc agagacggtg aaagtggtat
     5401 agctcagacc atagccgaac ggataaagcg ccccgttagc ttcatcaaaa taacgcgaag
     5461 tgtatttgtt cggcttgtcg gcattatacg gacgaccggt gttcagatgg ctgtagtaca
     5521 ccgggatctg cccgacagaa cgcgggaagg acatcggcag cttgccggac gggttgtaat
     5581 cgccaaacaa cacatcggca attgcattac cgccttcagt ccccgcaaac caggtttcca
     5641 gaatcgcatc agcctgctga tcttctttca ccagcgccag cggacgcccg ttcatcagca
     5701 ccagcaccag cggtttaccg gtggctttca gcgccgcaat caagtcacgt tggctttgcg
     5761 gaatagtgat atcggtacgg ctggaggcct cgtgcgccat cccctgtgct tcaccgacca
     5821 cagccaccac catatcagat tgtttcgccg tctgcaccgc ttcatcaatc atctcttgcg
     5881 gcgagcgcgg atcgactttg accgcttctt catactgatt caggaaatcg ataatgcctt
     5941 tgtcactggt aacgttcgcc cctttggcat acagcacttt accgttttca ccgacggcat
     6001 ttttaatccc ggtcagtacg gtcacggatt gatcggcaac accggctgcg gaccagctgc
     6061 ccatcacgtc gcgtttactg tccgccagtg gcccaaccac cgcaatggtg gccgattttt
     6121 tcagcggtaa cgtttcgaga cggtttttca gcaacaccag gctttcgcgc gccacttcac
     6181 gtgcttcttt acggtgcagg cggctttcgg cattggtatc caccgggtca gactctttcg
     6241 gccccaaatg gctgtatggg tcgttaaaca atcccatatc atatttaacg ttcagtacat
     6301 ggcgggcagc atcgtccagc tcttccatcg tcactttgcc ggatttgatc aacccaggca
     6361 gatacttcga gtaatactcg tcgctcatac tcatgttgat tccggatttc agcgccacgc
     6421 gcaccgcatc ttccgggtct gccgccgtgc catgtttaat cagctctttg attgcaccgt
     6481 gatcggaaac ggtgatgcct ttaaagcccc actggtcgcg cagaacatct ttcagcagcc
     6541 aggaatcgga ggtggctggc gtgccgttca gcgagttcag cgccaccatc accgcgccgc
     6601 tgcctgcgtc cagccccgct ttgtacggcg gcatataatc attaaacagg cgctgcggac
     6661 tcatatcgac ggtgttgtac tctttaccgc cttctaccgc gccgtatgcg gcaaagtgtt
     6721 tgacgctggt catcaccgag tagcgatctg ccgggctttt accctgcatc gcttccacca
     6781 tggttttacc cattgttgag gtgagatacg tatcttcgcc aaaaccttcg gaagcacgtc
     6841 cccagcgcgg atcgcgcgag acatcgacca tcggtgccca ggtcatattc aggccatcat
     6901 ctgccgcttc ataagcagag acacgtccga ccgttttcac tgcatcgagg ttaaaagacg
     6961 aggccagacc gaggctaatc gggaacaccg tgcgctgacc gtgcagcacg tcgtaagcaa
     7021 agaaaagagg aattttcagg cggcttaatt ccatcacctg atcctgcatg gcgcggatat
     7081 cctgacgggt tacggtgttg aaaatcgccc caacctgacc gtctttgatc atctcgcgga
     7141 tcgcctcttt cgggttatcc gggccgacgc tgattaagcg cagctgacca attttctcat
     7201 caactgtcat tttcttaagc aattcggtga cgaacgcatc ccgcgcttcg ggcgtgaatg
     7261 gatggttgcc gaataaatca tccgccagtg ctggctgcag ggccagactc accgcgattc
     7321 ctactgaaca tagccatttc atatggattt ttcttcctct gttgcccgac gaggcagcga
     7381 aaatggtgca aaaaccgtag tttgccataa gcatgatgga gagagaaaaa gaatgctcag
     7441 tttattgtct gaattttcaa aatattcact cgctgaattg tcatacaagg cgctattcta
     7501 gtttgtgata ttttttcgcc accacaagga gtggaaaatg tcttccatga caacaactga
     7561 taataaagcc tttttgaatg aacttgcccg tctggtcggt cattcacacc tgctcaccga
     7621 tcccgcaaaa acggcccgct atcgcaaggg cttccgttct ggtcagggcg acgcgctggc
     7681 tgtcgttttc cctagctcac tactggaatt gtggcgggtg ctgaaagcct gtgtcaccgc
     7741 cgacaaaatt attctgatgc aggctgccaa tacaggcctg accgaaggat cgacgccaaa
     7801 cggtaacgac tatgatcgcg atatcgtgat catcagcacc ctgcgtctcg acaagctgca
     7861 cgttcttggc aagggcgaac aggtgctggc ctatccgggc accacgctct attcgctgga
     7921 aaaagccctc aaaccgctgg gacgcgaacc gcactcagtg attggatcat cgtgtatagg
     7981 cgcatcggtc atcggcggta tttgtaacaa ctccggcggc tcactggtgc aacgtggccc
     8041 ggcgtatacc gaaatgtcgt tattcgcgcg tataaatgaa gacggcaaac tgacgctggt
     8101 gaaccatctg gggattgatc tgggcgaaac gccggagcag atccttagca agctggatga
     8161 tgatcgcatc aaagatgacg atgtgcgtca cgatggtcgt cacgcccacg attatgacta
     8221 tgtccaccgc gttcgtgata ttgaagccga cacgcccgca cgttataacg ccgatcctga
     8281 tcggttattt gaatcttcag gttgcgccgg gaagctggcg gtctttgcag tacgtctaga
     8341 taccttcgaa gcggaaaaaa atcagcaggt attttatatc ggcaccaacc agccggaagt
     8401 gctgaccgaa atccgccgtc atattctggc taacttcgaa aatctgccgg ttgccgggga
     8461 atatatgcac cgggatatct acgatattgc ggaaaaatac ggcaaagaca ccttcctgat
     8521 gattgataag ttaggcaccg acaagatgcc gttcttcttt aatctcaagg gacgcaccga
     8581 tgcgatgctg gagaaagtga aattcttccg tccgcatttt actgaccgtg cgatgcaaaa
     8641 attcggtcac ctgttcccca gccatttacc gccgcgcatg aaaaactggc gcgataaata
     8701 cgagcatcat ctgctgttaa aaatggcggg cgatggcgtc ggtgaagcca aatcgtggct
     8761 ggtagattat ttcaaacagg ccgaaggcga tttctttgtc tgtacgccgg aggaaggcag
     8821 caaagcgttt ttacaccgtt tcgccgctgc gggcgcagca attcgttatc aggcggtgca
     8881 ttccgatgaa gtcgaagaca ttctggcgtt ggatatcgct ctgcagcgta acgacaccga
     8941 gtggtatgag catttaccgc cggagatcga cagccagctg gtgcacaagc tctattacgg
     9001 ccattttatg tgctatgtct tccatcagga ttacatagtg aaaaaaggcg tggatgtgca
     9061 tgcgttgaaa gaacagatgc tggaactgct acagcagcgt ggcgcgcaat accctgccga
     9121 gcataacgtc ggtcatttgt ataaagcacc ggagacgttg cagaagtttt atcgcgagaa
     9181 cgatccgacc aacagtatga atccggggat cggtaaaacc agtaagcgga aaaactggca
     9241 ggaagtggag taaaaattac ggatggcata gtatcgccat ccgtcattca cttaatcgtt
     9301 ctgtgccgtc tgccccgccg ccgccatttg ggcggctttt tgttttttat agctcaacgc
     9361 tgctgctgga acaggcatca ctttaccggt ttcaatccag gtacgcaggc ggctggcatc
     9421 ggcaaaatgg gtatatttgc caaacgcatc catcactacc agcgccaccg gtttattatt
     9481 gataaccgta cgcatcacca gacaatggcc cgccgcattg gtaaaaccgg ttttggttaa
     9541 ctgaatattc cagttatcgc gatacaccag atggttagta ttgcggaacg gcagcgtata
     9601 cgtcgggtta gagaaggttg ccatatcttc ccgggtagta cttaactgcc cgatcaacgg
     9661 atattgtttg ctggcaatga gcagtttggt taagtcacgg gcagttgaaa cgttatgcac
     9721 cgacaatccg gtaggttcaa caaagcgcgt gttgttcatt ccgagcgatt tcgctttcgc
     9781 attcattgcc ttaataaagg ctttgtaacc accgggataa tggtgtgcaa ggcttgccgc
     9841 cgcgcggttt tctgaagaca tcagcgccag caacagcata tctttacggc tgatttcgct
     9901 attcagtcgt acgcgcgaat agaccccttt catctccggc gtctggctga tatccacttt
     9961 tagtttttca tccagcggca gtcgtgcatc cagcacaacc atcgcggtca ttaatttgct
    10021 gatagacgca atcggacgca ccagatccgg gtggttcgag tagatcactt tgttggtatt
    10081 cagatcaaca atcatcgcgc taccggaggc aatttcaggt tgtgaagcgg tgatagcggc
    10141 tgccgttttc gcaacggcct gcggtgccaa aggcacagcc agcatcaggg ccaggctaaa
    10201 taaagaaact cgaaatttcg gcatgatgag cattcagata gtggttcacg cgcacaggtt
    10261 gcgcaccgcc ggagtaagga tttactgagg ctagcgacgc catcataacg agcaaaaagt
    10321 gcgatcgtca aaggagaatc gtgaggaaat gctgcattgc tgacatttac gccagcaatg
    10381 caacgtcaaa gaactttcta gaacaaacga taaccgtagc cccatagtat aacggttagg
    10441 gcgagcagca cttccagtac cagcacgcca atcgccagcg tcgaactaga gaagctaagg
    10501 ccttcctctt tgttgatatt caggaagctc ggaataccaa ggtaaagcag gtagccggtg
    10561 taaaacagcg ccaccgtgcc gaccagcgca cacaaccaga ccagtggata aagcgccacc
    10621 aaaccgctta aaaacagcgg agttgcaacg tagcccgcga agaccatgca gtgcgcaagt
    10681 gacggacgct gcggataatt acgcgccatc caccagatga cccgccccat taccgcaacc
    10741 ccagccagca taacgccata aaacaggaca gccagcgcca gtccggtaaa ccaggataac
    10801 ttcaggatag tgccatcgcc aaaattccag ccaatctgtg tagtgccaat gaaggcgcaa
    10861 atcaccggaa tcgccgccat cagcaaaacg tggtgggtgt aatggtgaga aatcgtttcg
    10921 ttttcgcgat taatcacctg catttcacga tcgggatggg aaaacagtcc ccagacatgg
    10981 ctcataccgc ccccttgttg tgagttcatg aacctgacag ttcaagtata agtcagcttg
    11041 tgattatttt ttgttcgctc atgtaatttg caccgtctga tgtacgtcct ttttcagcgt
    11101 gaatgatttc cagggtgtat gcttacaggc aaccaaaggg agacagactg gcctatggat
    11161 ctcaatacac tcatctcaca atatggttat gccgcgctgg tgatcggtag cctggcggaa
    11221 ggtgaaaccg tgactttgct gggaggcgtt gcggcacatc aggggctatt aaagttcccg
    11281 ctggtggtac tttctgtggc gcttggcggc atgattggcg accaggtgct ttatctgtgc
    11341 gggcggcggt ttggcggcaa gctgttacgc cgtttctcga aacatcagga taaaattgag
    11401 cgggcgcaga aacttatcca acgccatccg tatctgtttg tcattggtac gcgctttatg
    11461 tatggctttc gggtgattgg cccgacgctg attggtgcca gccagctgcc gccgaaaatc
    11521 tttctgccgc tgaatattct cggcgcattt gcctgggcgt tgatttttac cactattggt
    11581 tacgctggtg gtcaggtgat tgcgccgtgg ttgcacaatc tcgaccagca tttgaagcac
    11641 tgggtctggt tgattctggt cgtggttctg gtggtgggcg tgcgctggtg gctgaaacgg
    11701 cgcgggaaga aaaagccgga tcatcaggcg taaaaccatt gccctgggta aggcgttcgc
    11761 gccgcatccg acatccggtg caccatgcct gatgcgacgc ttgtgcgtct tatcatgcct
    11821 accaaaattt tacccattga aataaccacg cacctcactg cgctattctg ggttgaactg
    11881 cggattcgcc aacataaagc cgccatccac tatcaacgac tgcccggtgg tgtaatttgc
    11941 gccctctgag cacagccacg ctaccaggct ggcaatctca tgcgttgcgc caaaacgccg
    12001 cagaggaatc gaaggctccg cgtcgggctt cacgtcgctg tcatccatgc cattcattgg
    12061 cgtggcgatc gccccaggcg cgactgcgtt caccaaaatc ttatgcctga ccagctccag
    12121 cgccatcgct ttggttaacc caccgagcgc atgtttagcg gctgtgtagg cgctggcatc
    12181 cggcagcggc gtatgttcat gtaccgatgt aatgttgatg atgcgaccgc cctgcccttg
    12241 tttcaccatc tggcgggccg caatttgtga gcataaaaat gcaccatcga catcaacggt
    12301 aaaaatcttg cgccactcat cgaaagccat atcaagaaac ggcgctttgg tcattgcgcc
    12361 cgcattattc accagcacat caatacgccc cagccgttga atgagtttct ccagcgcctg
    12421 cgcaccttct ggcagtttac cgagatccag ctgcacgatc tccgcacgta cgccgtggct
    12481 aactacctca cgcgcggtat cttttgcccc ttcttcatct gagtgccagg taatgccaat
    12541 atcaaaccct tgctgcgcca gtaataacgc gcactctttg ccgatccccg aatcggaggc
    12601 ggtaataatc gcaacctgtg ccatcgagtt ctccacttaa cgctgaataa acgttaagta
    12661 tagaaggcgc atatcatcag cgtttgtaac ccccgcccaa cgcaccagtg agttgaatgg
    12721 aggcatccag ccactgccct tgcaataaca ggccattggc ccgctcacgc agcgcgggga
    12781 ttctggcttc gctgacgcgg gaaccagcaa tgatgcccgc gttaaagcgc gcctgcgcaa
    12841 gacctaccac acgcaaggta tcgcgctcaa tttgcgcctg atgctggttt ttctccgcaa
    12901 gtgtctgaac ctgactggct gcccgcgcca cgtcattcac cgcttcaacc accgctttgt
    12961 tgtagctggc gatagacaag ttgctttcgg cttttgcgat atcgagattg gcgttaagac
    13021 gaccactatc gaaaatgggt agcgtcaggc ctgccgtaac gcccatttgc tgcgcggaat
    13081 gacggaacag atcgcttaag tgcaacgcat cctgttgcag gaaggccatc aggttgatgt
    13141 caggataaaa tgccgctttt gccgcatcaa tggtgcttag cgatgactca acgtaccagt
    13201 gcgccgcctg caaatctgcc cgccgggcca gtaaggagta ccccagttca tcaggaagct
    13261 ggcttgccac tttcggcaac gcgaccggtt taagcttcaa tgactttgtc tggttatttg
    13321 taagtgcgct taaccgtgcc tcaataattt tcattttccc cgcgacatcg ttgagctgct
    13381 gccgggtttt gctggcatta atatcggttt ccacaccttc aactgaagaa gtaatcccgt
    13441 tctgatatag ctggcgatcg gtcgcgataa tggtgttctg ctctttttct atttgctgca
    13501 agaccgtgtt taacgccgcc tgg
//
LOCUS       DAJVUJ010000113        13480 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.113, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000113 DAJVUJ010000000
VERSION     DAJVUJ010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13480
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.113"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(103..3324)
                     /gene="carB"
                     /locus_tag="OQQ48_002947"
     CDS             complement(103..3324)
                     /gene="carB"
                     /locus_tag="OQQ48_002947"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCS1953641.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(3342..4490)
                     /gene="carA"
                     /locus_tag="OQQ48_002948"
     CDS             complement(3342..4490)
                     /gene="carA"
                     /locus_tag="OQQ48_002948"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCS1953642.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
     gene            complement(4945..5766)
                     /gene="dapB"
                     /locus_tag="OQQ48_002949"
     CDS             complement(4945..5766)
                     /gene="dapB"
                     /locus_tag="OQQ48_002949"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCS1953643.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSRESHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNSL"
     gene            complement(5933..6847)
                     /gene="rihC"
                     /locus_tag="OQQ48_002950"
     CDS             complement(5933..6847)
                     /gene="rihC"
                     /locus_tag="OQQ48_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HCS1953644.1"
                     /translation="MHLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMSGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPELFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVKVALDLDVKGFQQWVAEVLALAS"
     gene            complement(6913..7863)
                     /gene="ispH"
                     /locus_tag="OQQ48_002951"
                     /gene_synonym="lytB"
     CDS             complement(6913..7863)
                     /gene="ispH"
                     /locus_tag="OQQ48_002951"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HCS1953645.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(7865..8314)
                     /gene="fkpB"
                     /locus_tag="OQQ48_002952"
     CDS             complement(7865..8314)
                     /gene="fkpB"
                     /locus_tag="OQQ48_002952"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCS1953646.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(8383..8877)
                     /gene="lspA"
                     /locus_tag="OQQ48_002953"
     CDS             complement(8383..8877)
                     /gene="lspA"
                     /locus_tag="OQQ48_002953"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCS1953647.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRLKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSR
                     AKKQ"
     gene            complement(8877..11693)
                     /gene="ileS"
                     /locus_tag="OQQ48_002954"
     CDS             complement(8877..11693)
                     /gene="ileS"
                     /locus_tag="OQQ48_002954"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCS1953648.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHFSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYHDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(11736..12677)
                     /gene="ribF"
                     /locus_tag="OQQ48_002955"
     CDS             complement(11736..12677)
                     /gene="ribF"
                     /locus_tag="OQQ48_002955"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HCS1953649.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYVVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(12685..12903)
                     /locus_tag="OQQ48_002956"
     CDS             complement(12685..12903)
                     /locus_tag="OQQ48_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HCS1953650.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGHFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            13006..13269
                     /gene="rpsT"
                     /locus_tag="OQQ48_002957"
     CDS             13006..13269
                     /gene="rpsT"
                     /locus_tag="OQQ48_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCS1953651.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT         3149 a         3807 c         3399 g         3125 t
ORIGIN      
        1 caaaagattt acttaatggg caaaaaaaac ggtcctcatc agagaaccgt attacttatt
       61 cgatcaaatt agcggatgaa aaatatccgc catgacacgc tattatttga tctgcgcgtg
      121 catttcctgc accgaaatta ctttttcggt cgcgtcggca ttcagcgcca tcgcggtagc
      181 gaaaccaccg ttcagggtgg tgtcataatg cactttatat tgcagcgcac tgcgacggat
      241 cacgcgggag tcttcaatcg cgcgacggcc tgaagtggtg ttgatgatgt aggtatattc
      301 gccattcttg atgcggtcct gaatgtgcgg acggccttca tgcaccttgt tcaccagacg
      361 cggattaatg cccgcttcgc ccagcacaat cgccgtgccg tgggtcgcat ccagctcgaa
      421 gccctgtttc agcagttttg ccgccaggtc caccacgcgt tctttatcgc cttcgcgcac
      481 ggaaagcaac gcacgaccgt gtttcttcat ggtggagttg ctgcccagct gcgctttggc
      541 aaacgcttca gcgaaggtgc ggcccacgcc catcacttcc ccggtggagc gcatttctgg
      601 ccctaacagc gggtcaacac ccgggaattt attgaacggc agcaccactt ctttcaccga
      661 gtagtacggc gggataactt ctttggttac gccctgctca gccagcgatt tgccagccat
      721 cacacgcgcc gccacttttg ccagcggtac gccggtggct ttggagacga acggaacggt
      781 acgcgccgca cgcgggttaa cttcaatcag gtagacttcg ttgtttttca ccgcaaactg
      841 cacgttcatc agaccgcgca cctgcaattc gaaggccagt ttctgcacct gctggcgcat
      901 cacatcctga atttcctgac ttaaggtgta ggctggcaga gaacatgcgg agtcaccgga
      961 gtgcacgccc gcctgctcga tgtgctccat gatgccgcca atcagcacca tttcgccgtc
     1021 gcagatggca tccacgtcaa cttctaccgc gtcatcaagg aagtgatcca gcaacactgg
     1081 cgcatcgtta gacacgctga ccgccgtctg gaagtagcga cgcaggtcag cttcgtcata
     1141 gacgatttcc atcgcccgac cgccgagaac gtaagacgga cgtaccacca gcgggtagcc
     1201 aatctctttc gccttctcta ccgccatttc aatagcggta acggtggcgt tcgccggttg
     1261 tttcagtttc agacgctcaa ccgcatgctg gaagcgttca cggtcttctg cacggtcgat
     1321 agcatccggg ctggtgccga taaccggtac gccagcagct tccagcgcgc gcgccagttt
     1381 cagcggggtc tgaccgccgt actgaacgat aacgcctttc ggcttctcaa tacgcacgat
     1441 ttccagcaca tcttccagag ttaccggctc gaagtagagg cgatcggaag tgtcgtagtc
     1501 ggtggagacg gtttccgggt tacagttaac cataatggtt tcgtaaccgt cttcgcgcag
     1561 cgccagcgag gcgtgtacac agcagtagtc gaattcgata ccctgaccga tacggttcgg
     1621 gccgccgccg agaaccatga ttttttcacg gtcggtagac ggattcgctt cgcactcttc
     1681 ttcataagtg gagtacatgt aagcggtgtc ggtggcgaac tctgccgcac aggtatccac
     1741 gcgcttataa accgggtgca ggtcatactg gtcgcgcagc ttgcggattt ccgcttcgcg
     1801 cacgcccgcc agttttgcca gacgcgcatc ggcaaagcct ttgcgtttca gctggcgcag
     1861 gaattcagcg ttcaggccag tgatgcccac ttccgctact ttctcttcca gacgcaccag
     1921 ttcttcaatc tgtaccagga accagcggtc aatattggtc aggttgaaca cgccgtccac
     1981 agacaggccc gcacggaagg catcggcgat gtaccagata cgctctgcgc cagcatcttt
     2041 cagttcgcga cggattttgg ttaacgcttc cgggtcatcg aggctcactt tcgggtcgaa
     2101 tccagtcgca ccgacttcca ggccgcgcag cgctttttgc agggattcct gctgcgtgcg
     2161 accaatcgcc atcacttcgc caaccgattt catctgagtg gtcagacggt cgttagcacc
     2221 agcgaatttt tcgaagttga agcgaggaat tttagtaacc acatagtcga tggacggctc
     2281 gaaggaggcc ggagtacgtc cgccagtgat gtcgttcatc agttcgtcga gggtgtaacc
     2341 caccgccagt ttcgccgcca ctttagcaat cgggaaaccg gtcgctttcg acgccagcgc
     2401 cgaagaacgg gacacgcgtg ggttcatttc gataacaatc aggcgaccgt ttttcgggtt
     2461 caccgcaaac tggacattgg aaccaccggt ttcaacgccg atttcacgca gcaccgccat
     2521 cgaggcgtta cgcatgattt gatattcttt gtcggtcagc gtttgggctg gcgcgacagt
     2581 gatggagtca ccggtatgga tgcccatcgc atcaaagttt tcgatagagc agacgatgat
     2641 gcagttgtcg tttttatcac gcaccacttc catctcgtac tctttccagc cgatcagcga
     2701 ctcatcaatc agcagctctt tggttgggga gagatccaga ccgcgggcgc aaatttcttc
     2761 aaactcttcg cggttataag cgataccgcc gccgctaccg cccatggtaa aggatgggcg
     2821 aataatgcac gggaagccca cgtcagcggc aaccgccagc gcttcttcca tcgtgtgtgc
     2881 gataccggaa cgcgcggttt ccagaccaat tttcttcatc gctacgtcga aacgacggcg
     2941 gtcttctgct ttatcaatcg catcggcggt cgcaccaatc atagtcacgc cgaactcttc
     3001 caacacgccc tgacgctcca gctccagagc gcagttcagc gccgtctgac cgcccatcgt
     3061 tggcagcacc gcgtccgggc gctctttttc aataatcttg cgtactactt cccagtgaat
     3121 cggctcgatg taggtcgcat cggccatttc cgggtcggtc atgatggtcg ccgggttgga
     3181 gttcaccaga atgacgcggt aaccctcttc gcgcagggct ttacatgctt gcgcgccaga
     3241 gtagtcaaac tcacacgcct gaccgataac aatcgggccc gcacccagaa tcaggatact
     3301 ttttatatct gtacgttttg gcatggctct tttactcctg attacttagc ggttttacgg
     3361 tactgctcaa ttaactcgat aaagtggtcg aacaacggcg ctgcgtcgtg tggcccaggg
     3421 ctggcttccg ggtgaccctg gaagctgaac gccggtttat cagtgcgatg aatgccctgt
     3481 aacgtaccgt cgaacaggga tttatgcgtg acacgcaggt ttgcaggtaa tgttgcttca
     3541 tccaccgcaa aaccgtggtt ctgggcggtg atcatcacca cgtttttctc cacatcttta
     3601 accggatggt tgccgccgtg gtgaccaaat ttcattttga cagtcttcgc accgctcgcc
     3661 agcgccagca gctgatgacc gaggcagatg ccgaataccg gaatatcggt ttcgaggaat
     3721 ttctggatgg cggtaatggc gtaatcgcac ggggccgggt cgccaggacc gttggagagg
     3781 aagatgccgt ctggattcat tttcagcaca tcttccgcag aagtttgcgc cggaacgatg
     3841 gtcaggcgac agcctctatc caccagcatc cgcaggatgt tgcgcttggc accaaaatca
     3901 taagccacga cgtggaacgg cagctcgtct tcttttttcg cttctggcag gccgccggtc
     3961 aacgtccagc tcccttgtgt ccagctatag gcttctgcgg tggtcacttc ttttgccaga
     4021 tccatgccat tcagacctgg gaacgcgcgg gctttttcta acgccaacgc cgcatccggg
     4081 ttatcgcctg cgataatgca gccattctgt gcgcctttct cgcgcagtaa acgcgtcagc
     4141 ttacgggtat cgatatcggc aatcgccacg atgttatggc gcttcaggta agaagagagg
     4201 tcttcggtat tacggaagtt gctggcaatc agcggcaggt cgcgaatcac cagaccttgt
     4261 gcatgtacct gagaagattc ttcatcggcg tcattagtgc cgacattgcc aatatgggga
     4321 taagtaagag taacgatttg acgagaatag gaaggatcag tgaggatttc ttgataaccg
     4381 gtcattgaag tattgaaaac gacttcccca accgccgaac ctgttgcccc tatggcccga
     4441 ccgtgaaact gggttccgtc ttccagaacc aatagcgctg acttaatcaa aacaccctcc
     4501 agagaatatt cactcacttt atttgcatat taattcacaa tgatgacgta aatcaatgca
     4561 aatctgctta ccgacgaatt tctggcaaac ggcggcattc tggagatatg ggcgctaaaa
     4621 gtcaacttaa tggcgggatt ttttatatta ttttgtggct catgcatgtt ttacaggcat
     4681 taagcgacaa aaagatcata taacctgttc tggaaaacgc ttgcacagca agaagattgc
     4741 atgaaataaa agggagcagg gaaaagtgga ccaaatggtc aaattttaca ttaaaacaac
     4801 aaaaacagaa gacaaaacat gtaatttttg gcacttatag acaatttaaa aacataacac
     4861 taaaaataaa agggccaaaa ttaaagcccc ttataatcaa taaattaaat gtgttatttt
     4921 tgcaccataa caaatatttt gtggttacaa actattgaga tcaagcacat ctcgcatatc
     4981 aaaaagacca ctttccttac cactcaacca caaagccgat cttaccgcgc cgttagcaaa
     5041 tgtcatgcgg ctggacgcct tatgggtgat ctccagacgc tcgccaatat cggcaaacat
     5101 cgcggtatgt tcgccaacga tgtcacctgc acgcacggtg gcaaaaccaa tggtgccagg
     5161 cacacgttca ccggtgtggc tttcacgact gtagaccgcg caatctttca gatctttatc
     5221 aagggcgtgg gcgatcgcct ctcccattgc cagtgcggtg cctgacggcg catcaacttt
     5281 atgtctatga tgtgcttcaa taatttcgat atcggtgtag tcacccatca ctttggctgc
     5341 tttctccagc agcttaagca tgacgttaac gccaacgcta aaattggcgg caaagacaat
     5401 cgcaatatcg gcggcagcgt cacgaatcgc ctgtttaccg gcttcgtcaa accccgtagt
     5461 gccgatcacc atccctttgc catgctggcg acaaaaagcg agatggttca gcgtaccttc
     5521 cggacgggta aaatcgataa acacatcaaa atcatctttt accgcatcga ggctgctttg
     5581 cacggtaacg cctgttttcc ctgcaccagc cagctcaccg gcgtcgctgc ccagtaaaga
     5641 agatccttcg cgctccagcg ccgcgcccag ctgcacgcct tctaatgcca gcgccgcctg
     5701 aatcaactgg cggcccatac gccccccggc tcccgcgatg gcaacgcgga tgtttgcatc
     5761 atgcatagct attctctttt gttaatttgc atagaccgtt ttcagagtaa ccagcgcatc
     5821 gctacgccgc cagccaaaaa cgctgataat tagaaattaa tgataaacag ggagggatat
     5881 cagtgaaagg catgagtcga tgaatgactg catgccgata acatgtgaca ggttacgacg
     5941 ccagagccag cacctcagcc acccactgct ggaagccttt cacatccaga tccaatgcca
     6001 cctttacatt ggctggtttg cccaggcaac cgtcgatatc aaccaccgtc gtgcctgagg
     6061 taaattcgcc ctgagtttcc actgccacaa aacacggttt gagagtgaac aattccgggc
     6121 gcaccagcca ggcaatagcg caaagatcgt gcatacgcag tccactttgc atactgccgc
     6181 tacggtagtg gctaaacagg gcgtggagca tttttccggt gcgattcaac tccggcaaag
     6241 tagcgagata gtcaggggtt aatattgcct gattggtgac atccagaccg ctcatgacga
     6301 tttcgatgcc gctacggaag acacaggcag ccgcttctgg atcggcagcg atattaaact
     6361 cggcgtttgg tgtgcagttg ccgcgaccag cagaaccgcc catgatcacc agacgacgaa
     6421 tatacggttt acattccggg cattgtgaaa gtaacagcgc aatattggtt aacgggccga
     6481 tggccaccag ggtaacgggc tctggtgcac gcatcagggc atcccgaatc gccagaaacg
     6541 ctggtatccc gagcggcttt cggttgtgct caacaaagtc gtagccagcc attcccgatt
     6601 cgccgtgcac agatgccgca tcacgcggtg cgcgtaccag cggcacagcg gctccctggg
     6661 cgagcggaat ctccgcattc cagaaatgca gcagttgcag ggcgttgcgg gtggttttct
     6721 caaccgagac attacccgcg acggtggtca tcagttgcag gtcgagttcg ggtgcaaaaa
     6781 tcgcggcggc aatggcgacg gcatcgtcaa tgccggggtc agtatcgagg aagataggta
     6841 aatgcatgtt ttctccataa aaaatgccgg taacaagacc ggcattttcg cataacttag
     6901 gctgctaatg acttaatcga cttcacgaat atcgacacgc agctctttcg gcacttcgaa
     6961 aacaatgttt tcttcacggc cttccagcgg aatggcttca ccaccgccca gctgctgcaa
     7021 acgtgccacc acattctgca ccagaatatc cggagccgat gcgcccgcag tcacgccgac
     7081 gcatttaacc tctttcaccc actcttcctg gatgtctttc gcatcgtcaa tcaaaaacgc
     7141 gcgtttgccc atacgctggg ccagctccgc cagacggttg gagttggagg agtttttcga
     7201 accgaccacc aacacaactt ccgcctgttc tgccagggcg cgtaccgctt cctgacggtt
     7261 agtcgtggcg tagcagatgt catctttgcg cggaccgaca attttcggga agcgtttacg
     7321 cagcgcgtcg atcacatcag acgtgtcatc caccgacagc gtggtctggg tcataaagga
     7381 gagcttctct tcgtttttga ccgtcagttt ccacacatcg tccggcgatt cgaccagata
     7441 cattccccct tccgggttac tgtactggcc cattgtccct tccacttccg ggtgcccggc
     7501 gtgaccgatg agaatagatt cttcgccacg gcgactggcg cgggcgactt ccatatgcac
     7561 tttggtcacc agcggacagg tcgcgtcaaa taccgtcaaa tcgcgacttt ttgcttcgtt
     7621 acgtaccgcc tgagaaacac cgtgtgcgga gaaaatcagg atcgcgccgt ccggtacttc
     7681 gctaatctgc tcaataaaga tagccccacg ctcacgcaag ctatcgacca catagcggtt
     7741 atgtaccact tcgtgacgga catatatcgg tgcgccgtaa atggccagcg cgttttcaac
     7801 aatgctgata gcgcggtcta ccccggcaca aaaaccacgc gggttggcca acaggatctg
     7861 catgttacgc ctccaatgcc ggatcgattt ccagcacttc aatatcaaaa tgaacggtct
     7921 gcccggccag aggatggttg aaatcaacgg taatggagtc gccgttaatt tcgcggatca
     7981 cgccaggcat ctcactgcca tccattgcgg taaaaagcat gattgcgcca atttctggct
     8041 cgcctgcatc cataaattca cggcgggaga agtactgaat caggtccggt gacggcacgc
     8101 caaacgccgc atctggctcc aacgagaagg tggttttatc gcccactttc agccccaaca
     8161 ggtgttgctc cagcccttca gaaagagaag catcacccag gcggaacagc gccggtttac
     8221 cgttgttgcg ggtagactcg gcggtggtgc catcgtcgag ttttagcgtg aagtgcacca
     8281 ggacggcgct attgctctgt acagattcag acatgcaggt ttgctcttat atttgaggat
     8341 ttttgccgga tgcggcgtaa acgccttatc cggcagggtt tattattgtt ttttcgctct
     8401 agaaggcaaa aaaccttcca gcacaatcag tgccgcaccg acacagatgg cagtatcggc
     8461 aaggttgaag gtggcgaagt gccagtcgcc gacgtagaag tcgatcatat cgacaacgaa
     8521 gccgtgccac aggcggtcga acaggttgcc cagcgcgccg ccaataatca gcgcgtaagc
     8581 gatattgttt agcttctgcg tggccttcaa gcgatacatc atcactgcca ggatcacgct
     8641 aataccaatc gcaataccgg caaagaacca acgctgccag ccgccgctat cggcaaggaa
     8701 actaaacgcc gcgccatagt tacgcgcata atgcagatta agcgacggga acagcgggac
     8761 cgtatccccc agagcaaagt tctggaggat caggtatttg ctgcccagat cgataatcag
     8821 cacgactacc accagccaca gccagcgtag ccctgttgaa cagatcgatt gactcatcag
     8881 gcaaacttac gtttttcacc gtcaccggcg acgttgctga cacagcggcc gcagatttct
     8941 gcgtgttccg ccaccttgcc gacatcctgg gtgtagtgcc agcagcgtgg gcacttctca
     9001 ccttcggctt tactcaacgc gactttcagc cctttgagta cttcgctctg ctgagcatca
     9061 gcaggtgcgt cgtgatagtc tgcaacggta gcgccggagg tcaacaggac aaatcgtaat
     9121 tcatcgccca gcgcggtcag tttcgccgcc agttccggtt ctgcatacaa ggttactgcc
     9181 gcttccagcg agccacccac tttcttgtcg gcacgcgctt gctcaatgac tttgttcact
     9241 tcgccacgca ctttcaacag ctcgtcccag aacgcatcgt tcatcgcttc gctgtctgcc
     9301 aggccaaaca ggccttcgta ccactcaccg gtgaagacgt atttttcacg ttcgcccggc
     9361 aggtagcccc acacttcatc agcggtgaag gagaggattg gtgccatcca gcgcaccagc
     9421 gcttctgcga tgtgatacag cgcagtctgg cagctacgac gcgccacact gtccgctttg
     9481 gcggtgtact gacggtcttt gatgatgtcg aggtagaagg aacccatctc aacggagcag
     9541 aagcgcatca gacgctgtac cacttcgtgg aaatcgtatg cttcgtacgc cttgaggatg
     9601 tcttcctgtg ccgctttcgc acaacctacg gcccagcgat ccagtaccac catctcttcc
     9661 ggtttcacca tatcttttgc tggatcaaaa ccgttcaggt ttgccagcag gaagcgcgcg
     9721 gtgttacgga tacgacgata gctatcggca gcacgtttca ggatctcgtc agaaacggcc
     9781 atttcaccgg tgtagtcggt tgatgccacc cacagacgca gaatatccgc gcccagtttg
     9841 ttcatcacat cctgcggcga aacggtattg ccgatggatt tagacatctt gcggccctga
     9901 ccatccacgg taaagccgtg ggtcagtacc tgacgatacg gcgctttgcc tttcatcgcg
     9961 gtggagatca ttagggaaga catgaaccaa ccacggtgct ggtcagaacc ttccagatac
    10021 atgtccgctg cgtgaccggc aaattccgga cgcacgtcaa caacagaaaa gtgggtagat
    10081 ccggagtcaa accatacatc caatgtgtcc ggcactttca cgtactgatc agcttcatcg
    10141 ccgaggatct ctttcgcatc aagatcccac cacgcctgga tgccgtcaac ttcaacgcgt
    10201 tttgccactt cttccattag ttcgagagta cgcggatgca gctcttccgt gtctttgtgc
    10261 acgaacagtg acatcggtac gccccaggtg cgctgacggg agatacacca gtcaggacgg
    10321 ttagcaacca tcgactcgat acgcgcctgg ccccagtccg ggatccactg cacgcctttg
    10381 atctctttca gtgactgcgc acgcagacct ttctgatcca tgctgacgaa ccactgcggc
    10441 gtcgcgcgga agatgatcgg cgttttgtga cgccagcagc acggatagct gtgctgcatt
    10501 ttctcaacgt gcagcagcgc gcctttttcc tgcagcagcg caacgacgat gtcgttcgct
    10561 ttgaagacgt tcacgccatc cagcgtcgga taagtgcccg gcagataagt gccgtccggg
    10621 ccaaccgggt tagcggtttc caggccgtat ttctgaccga tcacatagtc gtccgggccg
    10681 tggccaggcg cggtgtgaac ggcaccggta ccggcatcca gggtaacgtg atcgccgagg
    10741 attgccggaa cgtcgaagcc cataaacgga tgggtaaagc gcagcaactc aagctccgca
    10801 ccttttaccg tgccgagaat ggtgtaatcg gtcacgccga tacgctgcat tacgctttca
    10861 accagatctt tcgccagaat cacggcctga ccgtcgatct gcaccagcgc atagtcgaaa
    10921 tcaggtgcaa tagagattgc gcggttcgca ggcagagtcc acggcgtagt ggtccagatc
    10981 accagcgaga ttgggccgtt aacgttgctt acggcaaatt ttgctttcag tgcatcctga
    11041 tcgaccgcct gaaaagcaac gtcgatggac ggagaagttt tgtcgtaata ctcaacttcc
    11101 gcttccgcca gcgcagaacg gcagtcaacg caccagtgta ccggcttcgc gcctttgtgc
    11161 aggtgaccgt tgccgatgat tttgcccagc gcgcggatga tgttggcttc agttttgaag
    11221 tccatggtca ggtacgggtg cgaccagtcg cccagcacgc ccagacggat aaagtctttg
    11281 cgttgaccgt caacctgggt cgccgcgtat tcgcggcact tggcgcggaa ctcggcggcg
    11341 gtgaatttct cacccggctt accgtattct tgctctactt tcagctcgat cggcagaccg
    11401 tggcagtccc agccaggcac atacggcgag tcataaccgg aaagcccttt ggacttcacg
    11461 ataatgtctt tcagaatctt gttaaccgag tgaccaatat gaatgctgcc attcgcataa
    11521 ggagggccat catgcagaat gaaggttttt ttgccttttt tagccgcacg gatgatgccg
    11581 tacagatcat catcagtcca acgcgccagc attcccggtt cgcgcttggc gagatcgcca
    11641 cgcatcggga accctgtttc cggcaaattc agggttgatt tatagtcact catcagattc
    11701 tcggttccgt gtttcggttt gattacataa caggcttaag ccggttttgt tagcccaaaa
    11761 aattcgcggg cggttaattc atcacgcgca atctgcgctt tcagttcgtc cagcgacgca
    11821 aatcgctgct cattgcgtat ttttttacgc agcactactt gtatatggcg accgtaaagg
    11881 tccattgcaa catctaacaa atgcacttcc agctgctggc gaataccggc aaccgttggg
    11941 cgtgttccga tgtttgccac gccgggtaac ggcttttcac cgaggcccag cacttctacc
    12001 acataaaccc ctttcaccgg ggaaacctga cggcgtagcg gtacattcgc cgtcgggaaa
    12061 cctatagtgc gccctaattc atcaccgtgg actacacgcc cggagatggc aaacgggtgc
    12121 cccagtaaac tttctgccag agccagattg tcatccgcca gcgcctgacg cacagcggtg
    12181 ctgctaatac gcacgccacc ttcgcaaaaa gtttgcgtac tagtgatatc gaagccgtat
    12241 tccatgccag ctttctgtaa taacaagaaa tcgccttcac gaccagcgcc aaagcggaaa
    12301 tcatccccta cggcaagaaa ttttacgcgc aaatgcttca ccagaagatc gctgatgaaa
    12361 ttttgcgcgg ttaacgccgc gaaacgcctg tcgaaacgca cgcacagcac gtaatcaacg
    12421 ccacactctg caaggtaacg cagtttttcc cgcagccggg tcagtcttgc cggggcttta
    12481 tcggtagcaa acagttccag tggttgaggt tcaaaaagca tcaccatcac cggtaagttg
    12541 cgcttgcgcc cttcttcctg caagccctgt aacagcgcgc gatgaccgcg atgcacgccg
    12601 tcgaaattac caatagtcag cacacaccct tcttgcgggg cctggctgag attatgtatg
    12661 ccgcgtatca gcttcatgtc tggctcaaaa cagtgaaaat cgtccgagta taccttgtac
    12721 agcggtcaag gttaaccggc gattgagtac cgaaatcaga aagaagtggc ttttcattgc
    12781 catggcgcaa atcacgggaa gaaagtgacc gcctgctgca atttttatcg cggaaaagct
    12841 gtattcacac cccgcaagct ggtagaatcc tgcgccatca ctacgtaacg agtgccggca
    12901 cattaacggc gcttatttgc acaaatccat tgacaaaaga aggctaaaag ggcatattcc
    12961 tcggcctttg aattgtccat atagaacaca tttgggagtt ggaccttggc taatatcaaa
    13021 tcagctaaga agcgcgccat tcagtctgaa aaggctcgta agcacaacgc aagccgtcgc
    13081 tctatgatgc gtactttcat caagaaagta tacgcagcta tcgaagctgg cgacaaagct
    13141 gctgcacaga aagcatttaa cgaaatgcaa ccgatcgtgg accgtcaggc tgctaaaggt
    13201 ctgatccaca aaaacaaagc tgcacgtcat aaggctaacc tgactgcaca gatcaacaaa
    13261 ctggcttaat cgccaatttg ctgaagcttt gtgaaaaagc ccgcgcaagc gggttttttt
    13321 atgcctgctg cttttatgat gcgtgagaga acacactacc ggttgccaga tagtcaaaag
    13381 atgctggctt ttgcagacac gccgttacac tttctgaact cttcgtggct taagaatcgt
    13441 agaaccatcg gctgatacat tctgaacaag aatatctact
//
LOCUS       DAJVUJ010000114        13429 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.114, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000114 DAJVUJ010000000
VERSION     DAJVUJ010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13429
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.114"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..308
                     /locus_tag="OQQ48_002958"
     CDS             <1..308
                     /locus_tag="OQQ48_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCS1953652.1"
                     /translation="VLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNVINGLQFF
                     DQQIRWAKADLVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAILLIN"
     gene            507..788
                     /gene="ppiC"
                     /locus_tag="OQQ48_002959"
     CDS             507..788
                     /gene="ppiC"
                     /locus_tag="OQQ48_002959"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HCS1953653.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(875..2350)
                     /gene="ilvC"
                     /locus_tag="OQQ48_002960"
     CDS             complement(875..2350)
                     /gene="ilvC"
                     /locus_tag="OQQ48_002960"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HCS1953654.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRCHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            2500..3390
                     /gene="ilvY"
                     /locus_tag="OQQ48_002961"
     CDS             2500..3390
                     /gene="ilvY"
                     /locus_tag="OQQ48_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HCS1953655.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSSEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(3387..4931)
                     /gene="ilvA"
                     /locus_tag="OQQ48_002962"
     CDS             complement(3387..4931)
                     /gene="ilvA"
                     /locus_tag="OQQ48_002962"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HCS1953656.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRAVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(4934..6784)
                     /gene="ilvD"
                     /locus_tag="OQQ48_002963"
     CDS             complement(4934..6784)
                     /gene="ilvD"
                     /locus_tag="OQQ48_002963"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HCS1953657.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDTLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(6849..7778)
                     /gene="ilvE"
                     /locus_tag="OQQ48_002964"
     CDS             complement(6849..7778)
                     /gene="ilvE"
                     /locus_tag="OQQ48_002964"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HCS1953658.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(7798..8061)
                     /gene="ilvM"
                     /locus_tag="OQQ48_002965"
     CDS             complement(7798..8061)
                     /gene="ilvM"
                     /locus_tag="OQQ48_002965"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HCS1953659.1"
                     /translation="MMQYQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(8058..9704)
                     /locus_tag="OQQ48_002966"
     CDS             complement(8058..9704)
                     /locus_tag="OQQ48_002966"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HCS1953660.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLATTKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPELQQPLNINDWQQYC
                     AQLRDEHAWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(9707..9757)
                     /locus_tag="OQQ48_002967"
     CDS             complement(9707..9757)
                     /locus_tag="OQQ48_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1953661.1"
                     /translation="MNNSTKFCFSTFSTGN"
     gene            complement(9844..9942)
                     /gene="ilvL"
                     /locus_tag="OQQ48_002968"
     CDS             complement(9844..9942)
                     /gene="ilvL"
                     /locus_tag="OQQ48_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HCS1953662.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            10294..11814
                     /locus_tag="OQQ48_002969"
                     /pseudo
     CDS             10294..11814
                     /locus_tag="OQQ48_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
     gene            complement(11839..12177)
                     /gene="maoP"
                     /locus_tag="OQQ48_002970"
     CDS             complement(11839..12177)
                     /gene="maoP"
                     /locus_tag="OQQ48_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HCS1953663.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            12296..13135
                     /gene="hdfR"
                     /locus_tag="OQQ48_002971"
     CDS             12296..13135
                     /gene="hdfR"
                     /locus_tag="OQQ48_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HCS1953664.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQSARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSLL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(13230..13305)
                     /locus_tag="OQQ48_002972"
     tRNA            complement(13230..13305)
                     /locus_tag="OQQ48_002972"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13270..13272),aa:Trp,seq:cca)
     gene            complement(13314..13390)
                     /locus_tag="OQQ48_002973"
     tRNA            complement(13314..13390)
                     /locus_tag="OQQ48_002973"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13354..13356),aa:Asp,seq:gtc)
BASE COUNT         3114 a         3638 c         3478 g         3199 t
ORIGIN      
        1 tcgtgctcaa cggaaaaatt aatggcaacg catgtatcgt cgttccatta tcaaccactc
       61 gcgatcatga caagttaaat cgtggaatgc atgttgaaat agccagtaat gttatcaatg
      121 gcctgcaatt ctttgatcag cagattcgtt gggccaaagc cgacctggta caacaagtca
      181 gtcgaaaccg attaaataga gcaaggactt acagagggta tcttaatcaa tgtttgccac
      241 atgagttggt tgcagacata cagagggcgg tcatcaagtc aatcaatgcc atattgctca
      301 taaattgaca ataaaataac catccccgat aatgactttg tagcccatag aggccctaag
      361 ttttctcccc ttgagggacc tgcgaagccc ggattttccg ggcttttgcc atatatgtac
      421 cagcccctcc ctgactgaca attacgttag catctgcgga tcatgctaaa atcgccgcct
      481 gacaattatt cactcaaggc acgatcatgg caaaaacagc agcagcactg catatccttg
      541 taaaagaaga gaaactggct ctggatcttc tcgagcagat taagaacggt gccgatttcg
      601 gcaagctggc gaagaaacac tccatttgcc catcaggcaa acgcggcggt gatttaggtg
      661 aattccgcca gggtcagatg gttccggcgt tcgataaagt tgtgttctct tgtccggtac
      721 tggagccgac cggcccgctg cacacccagt tcggttacca catcattaag gtgctgtacc
      781 gtaactaata gcaaggcctt ctccaggaga aggccttgag tgttttctcc ctctccctgt
      841 gggagagggt cggggtgagg gcatcagcgc gcacttaacc cgcaactgca atacgtttca
      901 tatccgtcat atagccgcgc agtttcttac ctacctgctc aatcgcatgg cagcgaatcg
      961 cttcgttcac atcacgcagt tgcgcgttat ctaccgcacc ttccggaata gctttaccca
     1021 gatcgcccgg ttgcagctct gccataaacg gtttcagcaa cggcacacaa gcgtaagaga
     1081 acagatagtt accgtactca gcggtatcag agataaccac gttcatttcg tacagacgct
     1141 tacgggcgat ggtgttggca atcagcggca gctcgtgcag tgattcataa tatgcagact
     1201 cttcaatgat gccggaatcg accatggttt caaacgccag ctcaacgccc gctttcacca
     1261 tcgcgatcat cagtacgcct ttatcgaagt actcctgctc gccgattttg ccttcatact
     1321 gcggcgcggt ttcgaaggcg gttttgccgg tctcttcacg ccaggtcagc agtttcttat
     1381 catcgttggc ccagtccgcc atcataccgg aggagaattc gccggagatg atgtcgtcca
     1441 tatgtttctg gaacaggggt gccatgatct ctttcagctg ttcagacagc gcgtaagcac
     1501 gcagtttcgc cgggttagag agacggtcca tcatcagggt gatgccgccc tgcttcagcg
     1561 cttcggtgat ggtttcccaa ccgaactgaa ttagtttttc tgcgtatgcc ggatcggtgc
     1621 cctcttccac cagcttgtcg aagcacagca gcgagccagc ctgcaacata ccgcacagga
     1681 tggtttgctc acccatcagg tcagatttca cttccgcaac gaaggacgat tccagcacgc
     1741 ccgcacggtg accaccggtt gctgccgccc atgctttagc gatcgccatg ccttcgcctt
     1801 tcggatcgtt ttccgggtga acggcaatca gcgtcggtac gccgaatcca cgtttgtact
     1861 cttcacgcac ttcggtgcca gggcatttcg gcgcaaccat gactacggtg atgtctttac
     1921 ggatctgctc gcccacttcg acgatgttga aaccgtgcga gtaacccagc gccgcgccgt
     1981 ctttcatcag tggctgtacg gtgcgcacta cgtcggagtg ctgcttgtct ggcgtcaggt
     2041 taaccaccaa atccgcctgc gggatcagtt cttcgtaagt gcccacttta aaaccgtttt
     2101 cggtcgcttt acgccaggat gcgcgcttct cggcaatcgc ttctttacgc agagcgtagg
     2161 agatatcgag accagaatca cgcatgttca ggccctggtt cagcccctgt gcgccacagc
     2221 cgacgataac cactttttta ccctgaaggt agctcgcgcc gtcggcgaat tcgtcgcgcc
     2281 ccataaagcg acatttgccc agctgtgcca gctgctggcg caggttcagt gtattgaagt
     2341 agttagccat ggtgattcct cgtgatgttg tgcttcttat tgttcggttc gcgtttgcga
     2401 gattgaattc actatatgac aggaaattta ttgcggaaat tgatatattc acaacgtcac
     2461 attgcaattt ctgcaacgtc aacattgagg gctgctccat tggatttacg cgatctgaaa
     2521 accttcctgc atctggcgga aagccgccat tttggccgca gcgcgcgggc gatgcacgtt
     2581 agcccatcca cgctctcacg gcagattcag cgcctggaag aagatcttgg tcagccgcta
     2641 tttgtgcgcg ataaccgcac ggtgacgctg actgaagcgg gcgaagagct gcgcgttttc
     2701 gcccagcaaa cgctgttgca gtatcagcag ttgcgccaca ccatcgatca gcaagggccg
     2761 tcgctctctg gcgaattaca tatcttctgc tcggtgaccg ctgcctacag ccatctgcca
     2821 ccgattctcg accgcttccg cgcggaacac ccgtcggtgg agatcaaact aaccactggt
     2881 gatgcggcag atgcgatgga aaaggtagtc actggcgaag cggatctggc gattgccggg
     2941 aaaccggaga ctctgcccgg cgcagtggcg ttttcgatgc tggagaatct ggcagtagtg
     3001 ctgattgcgc ccgcgctgcc ctgcccggtg cgtaaccagg tttcgtcaga gaagccggac
     3061 tggtctacag tgccgtttat catggccgat cagggaccgg tacgccgccg cattgaactg
     3121 tggtttcgac gcaataaaat cagcaacccg atgatttacg ccacggttgg cgggcatgaa
     3181 gcgatggtat cgatggtggc gctcggctgt ggcgtggcgc tgttgccgga agtagtgctg
     3241 gaaaacagcc ccgaaccggt gcgtaaccgc gtgatgattt tagagcgcag cgatgaaaaa
     3301 acgccgtttg aactgggcgt ttgtgcgcag aaaaagcggc tgcatgagcc gctgattgag
     3361 gcgttctgga agattttgcc gaaccattaa cccgccaaaa agaacctgaa cgccggatta
     3421 ttggtttcgt cgtggcaatc gtagcccagc tcatttagcc gggtttcgaa atccggttca
     3481 tggtcgccga gttcgaacgc cgccagtacg cgcccgtagt cggtgccatg gctgcgatag
     3541 tggaacaaag aaatgttcca gtgcgtaccc agcgtgttga ggaagcgcag cagcgcgccc
     3601 ggtgattccg ggaattcgaa gctgtagagg cgttcctgca acggatgtga cggacgcccg
     3661 ccgaccatat agcgcacatg caacttcgcc atttcgtcgt cggagagatc caccacgctg
     3721 tagccgccgt cgttgagcat ctgcaaaatt tctttgcgct cttcgaggcc gcggctcagg
     3781 cgcacgccga caaagatgca ggcgtttttg gcatcggcaa aacggtagtt gaactcggtg
     3841 accgctcgcc cgccaagcag ttggcagaat ttgaggaagc tgcctttttc ttccggaatg
     3901 gtcaccgcca acaacgcttc acgctgttcg cccagttcgc agcgttccga gacgtagcgc
     3961 aggccgtgga agttcacgtt ggcaccggaa agaatatgcg ccagccgttc gccgcgaatg
     4021 ttgtgctggg cgatatattt tttcattccc gccagcgcca gcgcgccaga gggttccgcc
     4081 accgcgcgca catcttcgaa taaatccttc atcgccgcac agatcgcatc actatcgacg
     4141 gtgatgatgt cgtcgagata ctcctggcat aaacggaagg tttcgtcacc gatgcgtttt
     4201 accgctacgc cttcagcaaa tagccctacg cgcggcagat caaccggatg acccgcatcc
     4261 agcgctgctt tcaggcaggc ggagtcttcc gcttctacgg cgatcacttt gatttgtggc
     4321 atcagttgtt tgatcagcac cgcaacgccc gccgccagac cgccgccgcc gactggcaca
     4381 aatacgcggt cgagatgggc gtcctgctgg agcagttcca gcgccagcgt gccttgcccg
     4441 gcaatcacca tcggatggtc gaacggcggc acccaggtga acccctgctg ctgtgacagt
     4501 tcgatcgctt tagctttcgc ttcgtcaaag ttcgcgccat gaagcagcac ttcgccgccg
     4561 aatccacgta ccgcatcgac tttgatgtcg gcggtggcgg ttggcataac gatcagggcc
     4621 ttcacgccta accgcgcaga agaaaacgcg acgccctgcg cgtggttacc cgcagaagca
     4681 gtgatcacgc cgtgcgcttt ttgttcttcc gtcaggcccg ccatcatggc gtatgcgccg
     4741 cgcagcttaa agctgtgcac tggctggcga tcttcgcgct tcaccagaat gacgttatca
     4801 agacgcgacg acagtttttc cattttttgt agcggcgtaa cctgcgctgc ctcgtaaacc
     4861 ggcgcgcgca gcactgctct taaatattcg gcaccttccg gagcaccaga caggggttgc
     4921 gagtcagcca ttattaaccc cccagtttcg atttatcgcg caccgcacct ttgtcggcgc
     4981 tggttgccag gctggcgtag gcgcgcaggg caaaggaaac ctgacgttca cggtttttcg
     5041 gcgtccaggc tttgtcaccc cgagcttcct gcgcttcacg acgcgccgcc agttcggcat
     5101 cgcttacctg taactgaatg ccacggttcg ggatgtcgat agcgatcagg tcaccatctt
     5161 caatcagacc aatgctgccg ccgcttgccg cttccggtga gacgtggccg atggaaagac
     5221 cagaggtgcc gccagagaaa cgaccgtcgg tgatcagcgc acaggcttta ccgagaccca
     5281 ttgatttcag gaagctggtt gggtagagca tttcctgcat gccagggccg cctttcgggc
     5341 cttcatagcg gatcaccacc acatcgcccg cgacaacttt gccaccgagg atcgcttcta
     5401 ccgcgtcgtc ctggctttcg tacacttttg ccgggccggt gaatttgagg atgctgtcat
     5461 cgacgcccgc ggttttcacg atgcagccgt tttcagcaaa attaccgtag agcaccgcca
     5521 ggccgccgtc tttgctgtag gcgtgttcca gcgagcggat acagccgttt gcgcgatcgt
     5581 cgtccagcgt atcccaacgg caatcttgcg agaatgcctg ggtggtacgg atacccgcag
     5641 ggccggcgcg gaacatattt tttaccgcgt catcctgggt cagcataacg tcgtattgct
     5701 ccagcgtttg cggcaacgtc aggccgagca cgtttttcac atcacggttc agtaaccccg
     5761 cgcgatccag ttcgccgaga ataccgataa caccaccagc acggtgaaca tcttccatat
     5821 ggtatttctg ggtgctcggc gcaactttac acagctgtgg aaccttgcgg gaaagcttat
     5881 cgatatcact catggtgaag tcgatttccg cttcctgcgc cgccgccagc aggtgaagta
     5941 cggtgttagt cgatccaccc atcgcgatat ccagcgtcat ggcgttttca aacgccgcct
     6001 tactggcgat attacgcggc agtgcacttt cgtcgttttg ctcgtaataa cgtttggtca
     6061 attcaacaat gcgtttacca gcattaagga acagctgctt acggtccgcg tgggttgcca
     6121 gcagcgagcc gttgcctggc tgcgacaaac ccagcgcttc ggtcaggcag ttcattgagt
     6181 tagcggtaaa catcccggag caggaaccgc aggttgggca ggcggaacgt tcaacctgat
     6241 cgctctggga gtcagaaact ttcgggtctg cgccctggat catcgcatca accagatcga
     6301 gtttgattat ctgatcggac agtttggttt tcccggcctc catcgggccg ccggaaacaa
     6361 agatcaccgg aatattcagg cgcagggaag ccatcagcat ccccggggtg attttgtcgc
     6421 agttagagat gcagaccatg gcgtcggcgc agtgggcgtt gaccatatac tcaacggaat
     6481 cagcgatcag ttcgcgagat ggaagtgaat aaagcatccc cccgtggccc atggcaatcc
     6541 catcatccac cgcaatggtg ttgaactctt tggcaacgcc gccagccgct tcaatttgtt
     6601 cggcgaccag tttaccgaga tcgcgcagat ggacgtgacc cggtacaaat tgggtgaacg
     6661 agttcacaac cgcgataatc ggcttgccaa aatcggcgtc ggtcattccg gtggcgcgcc
     6721 acagcgcacg agcacccgcc atattacgac catgagtggt ggtggcggaa cggtacttag
     6781 gcatacttta tttactccca gtgtctgtct cgtaaatggg acggtgcgtg ccgtcccatt
     6841 ttttgtattt attgattaac ttgatctaac cagccccatt tatcttcggt ttcgccagtg
     6901 aagaggccga agaaggcttg ctgaatgcgt ttggtaaccg ggccacaacg gccttcgcca
     6961 acctgaatac cgtctacgct gcgcactggc gtgatttctg ccgcagtacc ggacataaac
     7021 acttcatccg ccaggtacag ggattcgcgc gacagcacct gctcacgcac ttcaattccc
     7081 agctctttcg ccagtttgat gatggcatca cgggtaatac ccggcagcgc ggatgaggta
     7141 aacggtgggg tgaacagcac gccgtctttc acttcaaaca ggttttcacc tgcgccttca
     7201 gagatgtaac cgttcacatc cagcgcgata ccttcctgat aaccgtggcg gcgcgcttcg
     7261 ctacccacca gcagggaaga gaggtagtta ccaccggctt ttgccgccgt cgggatggtg
     7321 tttggtgctg cgcggttcca ggaggaaacc atcgcatcga tcccctgctc cagcgcttct
     7381 gcgcccagat acgctcccca cgggaaagca gcgataatca cgtcggttga gtatcccgct
     7441 ggcgggttta ctcccatgcc aacatcaccg acgaagatca gcggacggat ataggcgctg
     7501 gtgagattat ttttgcggat cacgtcacga caagcttcca tcagctcatc aatgctctgc
     7561 gaaaccggga agcgatagat tttggcggag tcatgcagac gctgcatatg ctcacgatgg
     7621 cggaatacaa ccggtccttt gtgcgagtcg tagcaacgga tgccttcaaa aaccgaagtg
     7681 ccatagtgca gcgcgtgcga catcacatgc accttcgcgt cttcccagcg aaccatctcc
     7741 ccattgaacc aaatgtaatc agctttcttc gtggtcattt ttatattcct tttgcgctca
     7801 ggcgcggatt tgttgtgatg tggttgtgct ctggcagatg gcaacgtgtg cgacgtccac
     7861 cagtttattt aactgactaa acagtaagtc gaccgaccgt gggctggcaa cggtcaattc
     7921 gatatttata ttttgtgcat cgctggcggc ggccatattc attgagcaga cgtggaaacc
     7981 acgatgacgc accacgcgta aaacacgttc taaggtttcc ggattgaagc gagccgatac
     8041 attgacctga tattgcatca tgataatttc tccaacattt ctgaattact ggcgccaggc
     8101 ggcaccagcg gccagacgtt ctcaagttcg tcgattgaga catgaagcag gtatggccca
     8161 tcactgttca gcatggtgtc gagtgccgct tcaacctggt ctttacgggt gatgtgttgg
     8221 ccagggatgc cgaaggcgct ggctaacatg aggaaatcgg ggttatcagt aagggtggtt
     8281 tcgctgtatc gttcctgaaa aaacagttgc tgccattgtc gaaccatccc taaccgttgg
     8341 ttatcgagta agacgatttt caacggtaac tgcttgcgtt ttacggtgcc cagctcttgc
     8401 acattcatca tgaaagagcc gtcaccggag atacagacga cggtatcgtt cggtcgcgcg
     8461 acttgtgcgc caaccgccgc tggtaaacca aaacccatag tgcctaagcc gctggaggtg
     8521 atgaaatttt ccgggcgagt gtgggcgatg tgttgcgcag cccacatctg gtgctgcccc
     8581 acatctgtgg tcacgacgca atccgcaggt ttacgatccg acagttgttt taacaacaac
     8641 ggcgcgtaga tagcgtcgcc gggatggtcg taacgccagg catgttcatc acgcagctgc
     8701 gcgcagtatt gctgccagtc attgatattt aacggctgct gtaattctgg taacagagca
     8761 tttaaatcac cttgtaatgc cacatgtgcc tgacgcagct tgttcatttc tgccgggtcg
     8821 atatccatat ggataacgct ggcgtgtggt gcgaaggtgt tcagtttgcc ggtcacccgg
     8881 tcatcaaaac gtgcacccac ggcgatcagc aagtcgcact cctgcaccgc gaagttcgcc
     8941 gctttggtgc catgcattcc cagcatgccc agatagtacg gataatctgc ttcaactgcg
     9001 cccagccctt tcagcgtgca ggtggcaggc atttttgtgg tagcgagaaa ttctcgtaaa
     9061 gcaggaactg cctgcgccat acccacgcca ccaccaacgt acagcatcgg tttttgcgct
     9121 tttgccagca tctggcgcgc ttgctcaact tcggcatgtg ggaaagtcac ttcgttttca
     9181 acggtggtga accacggttc caggtcaccg ctggctagct ggatatcttt tgggatatcg
     9241 accagaaccg gaccaggacg acctgagctg gcaacgtcga atgcttcagc cataatgcgc
     9301 ggcaactctt ccagcgactg caccagaaag ctgtgcttgg tacaggctaa cgacaatccc
     9361 aggacatcca cttcctgaaa tgcatcagtg ccgataaacg gtgcggacac ttgaccggtg
     9421 atggcaacaa cagggataga atctaacagt gcgtccgcaa gcccggttat caggttggtt
     9481 gcgcccggac cagacgtggc gatacatacg ccagttttgc cggtagcacg ggcataaccg
     9541 atagccgcca ttgccgcacc ctgctcatgt cggcacagca agtgctccac gccgccgtca
     9601 tacaatgcat cgtaaaccgg cataattgcg ccacccggat aaccgaaaac ggtgttcaca
     9661 ccctgtgccc gcaacgcatg taccacccac tgtgcgccat tcatagttag ttccccgtgc
     9721 tgaatgttga gaaacagaat tttgtgctgt tattcattgt ctgctcctcg gttatgtttt
     9781 taaggtcaaa aaaaaccccc ggacctttcg gtgcgggggt cttagttcgt taaggcttga
     9841 tttctaagcc tttcctcgtc caagtgcagc cccgcacggt gggataataa tcaccaccac
     9901 gctaatcacg accaggctaa tcactcgtag aagggctgtc attttgtctt ttcttgcatc
     9961 ttgttcgaag gaatgcctaa agagttacca taggttttgt aaatagtaca agatattttt
    10021 tggccgattt ttatgatgcg tttaataatt tcaatataag ttgttgtttt ttaaaaataa
    10081 aatagtgagt aaaaaaattc aatttcagta tgaatgttgt tcccttcctc gtagttcatc
    10141 ctttgtccgc tgcatttttg cgagcatcat tccaccgcca gaacaactca acttcacttg
    10201 cacaaataaa caggaatcag ccactaaaat tcacgaatta gcgggctaca gcagcgatat
    10261 cttatgcaca taatggtgac tcaaggaggg cttatgtcac tgtcaattgt tcatacccgc
    10321 gcagccctgg gagtaaatgc gcccccgatc actgttgagg tacatatcag taaaggtcta
    10381 cccggcttaa cgaaggtggg cttaccagaa acaacggtaa aagaagctcg cgatcgcgtg
    10441 cgcagcgcca ttatcaatag cggatatgaa tatccggcga aaaaaatcac catcaacctg
    10501 gctccagctg atctgccaaa agaaggggga cgatatgatt tacctatcgc cattgcgttg
    10561 ctggcggcct cagaacagct tacagccaat aagttagatg aatatggatt agtcggagaa
    10621 ctggcgctta caggcgctct gcgtggcgtt cccggcgcaa tctccagtac aactgaagct
    10681 attaagtcgg gcagaaaaat tatcgtcgcg aaagataacg aagatgaagt ggggctaatt
    10741 aacggtgaag gatgcctgat agccgatcat ctgcaagctg tctgtgcgtt tctggaaggt
    10801 aagcacgctc tcgaacgccc gaaaccaact gatgcagtat cccgggcgct acaacatgat
    10861 ctcagtgatg ttatcggtca ggaacaagga aagcgaggac tggaaattac cgccgctggc
    10921 gggcacaacc ttttactgat tgggccgccg ggaacaggta aaacaatgct cgccagccgt
    10981 attaatggcc tgttgccaga tttaagcaat gaagaggcac tggagagtgc tgcgatatta
    11041 agtctggtaa atgctgaatc agtacaaaaa caatggcggc agcgcccgtt ccgctcacct
    11101 catcacagtg catcgttaac tgcgatggta ggcggtggcg caattccagg gcccggtgaa
    11161 atttcgctgg cgcataacgg cgtgcttttt cttgatgagt tacctgaatt tgaaaggcgt
    11221 acactggatg ccttgcgaga gccgattgaa tccgggcaga tccatctttc acgcacgcga
    11281 gcaaaaataa cctatccagc ccgtttccag cttgtcgcgg cgatgaatcc cagccctacc
    11341 ggacattatc agggaaacca taaccgctgc acaccagagc aaacgctgcg ttatctcaac
    11401 cgactctctg gtccctttct cgaccgcttc gatctctcac tggagatccc attaccgcct
    11461 cccggtattt tgagtaaaac ggtagtgccg ggagaaagca gcgccaccgt taaacaacgc
    11521 gtaatggccg ccagagagcg ccaatttaag cggcagaata aactgaacgc ctggctggat
    11581 agtccggaaa tacgccaatt ctgcaaactt gagagcgaag atgcgatgtg gctggaagaa
    11641 acgctgatcc atctggggtt atcgattcgt gcctagcagc ggttattgaa agttgcacga
    11701 accattgctg atattgatca gtctgacatt atcacacgtc aacatttgca ggaggcagtt
    11761 agctatcgag cgattgaccg tttgctcatc catctgcaga aactactgac ataaaaaaag
    11821 ggcatttcgc cctttttatt aatcgtcaga atcggtgtag tcttcagcac cttcaacctg
    11881 cggtttaccg ccggaaaggg tgtgaaaacg ttttggacgc ttaatacgcg tcatatactt
    11941 ggaccacacg cgttctgctt ctgtcactgg ctcacgttcg ccacggcata ctgctacgaa
    12001 gagtttctct tcctcggtaa ccggctcgcg tttgccaaga tccaactcat tgaaggcata
    12061 accatgacgc tcaagcagtt gtgcctcttt gatggtgaaa tcaccatgac gagagaatcc
    12121 acgtggataa tgtttattgt cgaaatatcg attagtcgtc gtaaagcttt ccgccatcct
    12181 gcacgctcct aattctttga ccgagctagt tatggcgcgg agtattagtt acgcttgaca
    12241 gagtgtaaaa caaaacattt aaatcataac gacaaataat tttgcggaga gcactgtgga
    12301 tacggaattg ttaaaaactt tcctggaagt tagccgaacg cgtcactttg gtcgagcggc
    12361 tgaatcgctc tatctgaccc agtcagcagt gagctttcga atcagacaac tggaaaatca
    12421 actgggtgtg aaccttttca cccgccacag aaacaatatc cgtttaaccg ctgccggtga
    12481 aaaactactg ccttatgcag aaacgctcat gagcacctgg cagtccgccc gcaaggaggt
    12541 ggcgcatacc tcacgacata acgagttttc tatcggtgcc agcgcctcat tgtgggaatg
    12601 tatgcttaat cagtggctgg gacgtttgta tcaaaatcag gatgcccata caggcttaca
    12661 gttcgaagcg cgaattgccc aacggcagtc tctggtaaaa cagctgcatg aacgccagct
    12721 tgatcttctt attaccactg aagcgcccaa aatggacgaa tttagtagtc tgttgctggg
    12781 atatttcact ttagcgcttt ataccagtgc cccttcaaaa ctaaagggag atcttaatta
    12841 tctgcgactt gagtgggggc cagattttca acagcatgag gcaggtttga tcggtgctga
    12901 cgaagtgccc attctgacaa ccagttctgc tgaactggca cagcaacaga ttgcgatgct
    12961 taatggttgc acctggctac ccgtcagctg ggcgcgtaaa aaaggcggcc tgcataccgt
    13021 tgtcgatagc acaacacttt cacggccgct ttatgccata tggctgcaaa atagcgataa
    13081 aaatgcgttg attcgcgatc ttttgaaaat taacgtgctg gatgaagtgt attaatatga
    13141 atggctggca aggatgccgg tagaaggatt tacttcggag agggttattt cagataaaaa
    13201 aatccttagc tttcgctaag gatgatttct ggcaggggcg gagagactcg aactcccaac
    13261 acccggtttt ggagaccggt gctctaccaa ttgaactacg cccctaatta gggtggcgga
    13321 acggacggga ctcgaacccg cgaccccctg cgtgacaggc aggtattcta accgactgaa
    13381 ctaccgctcc accgaattct tttacaacca ccggttttat gaccggctt
//
LOCUS       DAJVUJ010000115        13367 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.115, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000115 DAJVUJ010000000
VERSION     DAJVUJ010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13367
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.115"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(171..1094)
                     /gene="dmlR"
                     /locus_tag="OQQ48_002974"
     CDS             complement(171..1094)
                     /gene="dmlR"
                     /locus_tag="OQQ48_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HCS1953665.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYPNFSLEHA"
     gene            1197..2282
                     /gene="dmlA"
                     /locus_tag="OQQ48_002975"
     CDS             1197..2282
                     /gene="dmlA"
                     /locus_tag="OQQ48_002975"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HCS1953666.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFDLSFEQMG
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YINLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTYATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGNATTPQVADAICKIILR"
     gene            2532..4142
                     /gene="yeaV"
                     /locus_tag="OQQ48_002976"
     CDS             2532..4142
                     /gene="yeaV"
                     /locus_tag="OQQ48_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HCS1953667.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVIPAGTVFKGDN"
     gene            4174..5298
                     /gene="yeaW"
                     /locus_tag="OQQ48_002977"
     CDS             4174..5298
                     /gene="yeaW"
                     /locus_tag="OQQ48_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
                     /protein_id="HCS1953668.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTSANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            5354..6319
                     /gene="yeaX"
                     /locus_tag="OQQ48_002978"
     CDS             5354..6319
                     /gene="yeaX"
                     /locus_tag="OQQ48_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HCS1953669.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHVI
                     VQMSDGDNQYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGNRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALNEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(6373..7488)
                     /gene="rnd"
                     /locus_tag="OQQ48_002979"
     CDS             complement(6373..7488)
                     /gene="rnd"
                     /locus_tag="OQQ48_002979"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HCS1953670.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     SAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLTLVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(7569..9254)
                     /gene="fadD"
                     /locus_tag="OQQ48_002980"
     CDS             complement(7569..9254)
                     /gene="fadD"
                     /locus_tag="OQQ48_002980"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HCS1953671.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGVGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAVVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(9459..10040)
                     /gene="yeaY"
                     /locus_tag="OQQ48_002981"
     CDS             complement(9459..10040)
                     /gene="yeaY"
                     /locus_tag="OQQ48_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HCS1953672.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGRSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(10080..10775)
                     /gene="tsaB"
                     /locus_tag="OQQ48_002982"
     CDS             complement(10080..10775)
                     /gene="tsaB"
                     /locus_tag="OQQ48_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCS1953673.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWRGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPATEDMLPIACQIFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(10833..12743)
                     /locus_tag="OQQ48_002983"
     CDS             complement(10833..12743)
                     /locus_tag="OQQ48_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HCS1953674.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            12875..13219
                     /locus_tag="OQQ48_002984"
     CDS             12875..13219
                     /locus_tag="OQQ48_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCS1953675.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT         3198 a         3555 c         3335 g         3279 t
ORIGIN      
        1 accagatagg tccagtaatt cagaaccccg tattcagcga acacattaac ctctttaatt
       61 atctttcgat catgcgcgat taaaggtgaa tatgctaacc aatctgtagc ggcttagaaa
      121 ggagaaaatc aggttttaac ccgatatcaa cccgataatt gaatcattaa tcaggcatgc
      181 tccagtgaaa aattcgggta gtgctcggca aaatactggc gtaaaaattc taccgttatc
      241 cgcactttcg ctgacgtcgc cagccttgaa acataaacgg cccagacgtt cgctggctgg
      301 taatattccg gtagcacttg cactaaatga ccactggcaa tgttttcgct aacatcccac
      361 caggaacgca gagcaatccc ctgcccatcg agacaccact gatggacaat ttctccatga
      421 ttagacgaca gtgggccggt gactttaatc gcatggtggc cttctttatt gcgtaattgc
      481 cagacaccaa aaggatgatc gcgctcttta atgaccaggc acggcaatgc agaaagatcg
      541 gttaaatgtt tcggcgcgcc atgttgcgca ataaattccg gcgacgcgca gagaatgcgg
      601 taattggtcg ccagtttgcg ggcgattaaa ttaggggcga tgtcatcgcc aatgcgaata
      661 tcgagatcga caccttcatt gaccaaatcg accagccggt cttccacatc aaaacgtaat
      721 tccagttgcg gatacgcctt cgccagcgct gataacgccg gagccaccac ctgtcgtcca
      781 aaaccaaagc tgctgataat acgcagcatc ccctgtggca cctgacgcac gtcagaaagt
      841 tcgtccatca tctgaccgac atcctgcaaa atccgctgcg cccattcata aatccgctct
      901 ccttcttcgg taatggtgac gcggcgggtg gtgcggtgta acaacaccac gtttagcgtt
      961 tgctccagca aggcgatgcg cttgctgacg aacgccggtg aaacgcccag ttcttccgcc
     1021 acggcggcaa aaccagcccg acgagccacc agcataaaga cgcgcaaatc attcagcagc
     1081 ggtaaattat tcatgattcg tgttttatgt ttcaccagtt acggggatta attccttttc
     1141 cgtcaattat aggatggtga tgttgtcaat tttgatggtc aggaagtgag aacccaatga
     1201 tgaaaacgat gcgtattgct gcgatcccgg gagacgggat tggtaaagaa gtccttcctg
     1261 aagggattcg cgtgttacag gctgccgctg agcgctgggg cttcgacttg agttttgagc
     1321 aaatggggtg ggcgagctgc gagtattaca gccatcacgg taaaatgatg ccggacgact
     1381 ggcatgagca acttagccgt ttcgacgcca tctattttgg tgccgtcggc tggccggata
     1441 ccgttccgga ccatatttcg ttgtggggtt cgctgctgaa atttcgtcgt gaattcgacc
     1501 agtacatcaa cctgcgcccg gttcgtctct ttcctggcgt tccctgcccg ctggcgggaa
     1561 aacagcctgg cgacatcgat ttttacgtgg tcagggaaaa caccgaaggc gaatattcct
     1621 cgctcggcgg tagagtgaat gaaggtacag agcatgaagt cgtcattcag gaatcggtat
     1681 ttacacgtcg tggtgtcgat cgcattttgc gttatgcctt cgaacttgcg caaagccgcc
     1741 cacgtaagac gctaacttat gccactaaat cgaacggttt agccatcagc atgccgtact
     1801 gggatgagcg agtggaagca atggccgaga attacccgga gatccgctgg gacaagcagc
     1861 atattgatat tctctgcgcg cgttttgtga tgcagccgga acgcttcgat gtggtggtgg
     1921 cgtccaattt gtttggcgat atcctttccg atcttggccc ggcctgcacc ggcaccattg
     1981 gcattgcccc atccgccaac ctgaatccgg aacgcacttt cccgtcgctc ttcgagcctg
     2041 tccacggttc cgcgccggat atctacggga aaaatattgc taaccctatc gccacgattt
     2101 gggccggggc aatgatgctc gattttctcg gcaatggcga tgagcgtttc cagcaagcgc
     2161 ataacggtat tctggcagca attgaagaag tgattgctca cgggccgaaa acacctgata
     2221 tgaaaggcaa tgccaccacg ccacaggttg ccgacgcgat ttgcaaaatt attttgcgtt
     2281 aaggtcaaac cagtttattt gaaccgcgtc actgacgcgg ttttttattc gttctttgca
     2341 gtaaataacc tgcgtcattt caccttttat tgtttccgtt tcgtgtttta tggctttccg
     2401 cattcttaat tgtttaattt atgtaatatt caaataatca atttgttaaa cacaacatac
     2461 aactcattac cctgcaaata attacaactg gcaaaagcca gagacgggct tttatgccca
     2521 ggagatgact catgatgagc aatgttaaga aaaaagatgt gccgctgata agcatcagcc
     2581 tggtggccat tcttttcatc gcagctgcat taagcctttt cccacaacaa tcggccgacg
     2641 cggccaacgc aatatacact tttgttacgc gtacgttagg ttccgccgta caggtattgg
     2701 ttttgctggc aatgggactg gtgatttatt tagccaccag taaatacggc aatattcgtc
     2761 ttggcgaagg aaaaccggaa tacagcacgc tctcctggct gtttatgttt atttgtgccg
     2821 gtttaggttc ttctacgctt tattgggggg ttgctgaatg ggcctattat tatcaaacac
     2881 ctggattaaa tatcgcaccg cgttcacaac aggcactcga atttagcgtt ccctactctt
     2941 tcttccactg gggcatcagc gcctgggcaa cttatacgct ggcctcatta atcatggctt
     3001 atcactttca tgtgcggaaa aacaaaggtc tgagcctttc cggcattatt gctgctatta
     3061 ccggcgttcg cccgcaaggc ccatggggaa aactggttga tttgatgttc ctgatcgcca
     3121 ctgtcggcgc actgactatt tcccttgttg tcaccgcagc aacctttact cgtggacttt
     3181 ccgcactgac aggtttaccc gacaacttca ccgtgcaggc atttgtgatc ctgctttctg
     3241 gcggcatttt ttgcctaagc tcgtggattg gtatcaacaa cggtttgcaa cgtctgagca
     3301 aaatggttgg ctggggcgcg ttcctgctgc cattactggt gctgattgtc ggcccaaccg
     3361 aatttattac caacagcatc atcaatgcca tcggcctgac cacgcaaaac ttcctgcaaa
     3421 tgagcttatt caccgatccg cttggcgatg gttcatttac ccgcaactgg accgttttct
     3481 actggctgtg gtggatctca tacacccctg gcgtagcaat gtttgtcacc cgcgtttccc
     3541 gcggtcgtaa gattaaagaa gttatctggg gactgatcct cggcagcacc gtcggttgct
     3601 ggttcttctt cggcgtaatg gaaagctatg ccattcatca gtttatcaat ggcgtaatca
     3661 acgtcccaca ggtgctggaa acactgggcg gcgaaacagc tgtacagcaa gttctgatgt
     3721 cgttgccagc cggtaaattg ttcctcgccg catacctggg cgtgatgatt attttccttg
     3781 cctcgcatat ggatgcagtg gcctacacca tggcggcgac cagtacgcgt aatctccagg
     3841 aaggtgacga tcctgaccgt gggctgcgtc ttttctggtg cgtggtgatc actctgatcc
     3901 cgctttccat cttgtttacc ggtgcttcgc tggaaacgat gaaaaccacc gtcgtgctca
     3961 cagcccttcc cttcctcgtc attttactgg tgaaagtcgg cggatttatt cgctggctga
     4021 aacaggatta cgccgacatt ccggctcatc aagttgaaca ttatctcccg cagacaccgg
     4081 ttgaagccct ggaaaaaaca ccagtgatcc ctgcgggaac cgtattcaaa ggcgacaact
     4141 gagcgccatc atcctaacga taaaggtatc cctatgagca atctgagccc tgactttgta
     4201 ctacccgaaa atttttgcgc taacccgcaa gaggcgtgga ccattcctgc ccgtttttat
     4261 accgatcaga acgcgtttga acacgaaaaa gagaacgtct tcgccaaaag ctggatttgc
     4321 gtcgctcaca gcagcgaact ggcgaatgcc aatgattatg tgacgcgtga gatcattggc
     4381 gaaagcatcg tgctggtacg cggtcgtgat aaggttttgc gcgcgttcta taacgtgtgt
     4441 ccgcaccgtg gtcatcagtt gttgagcggt gaaggaaaag caaaaaatgt gattacctgc
     4501 ccgtatcacg catgggcatt caaactcgat ggcaatctgg cccatgcacg taactgcgaa
     4561 aacgtcgcca atttcgatag cgacaaagcg caactggttc cggtgcgtct ggaagaatat
     4621 gccggattcg tcttcatcaa catggacccc aacgccacca gcgtagaaga tcagttaccc
     4681 ggtctgggcg cgaaagtgct ggaagcctgc ccggaagtcc acgatctgaa actggcggcc
     4741 cgctttacca cccgcacgtc tgccaactgg aagaacattg tcgataacta tctcgagtgc
     4801 tatcactgtg gtccggcgca tccaggtttc tccgactccg tacaggttga tcgttactgg
     4861 cacaccatgc acggtaactg gacgctgcaa tacggtttcg ccaaaccgtc cgaacagtcg
     4921 tttaaatttg aagagggtac ggatgcggca ttccacggtt tctggctgtg gccgtgcacg
     4981 atgctgaacg tcaccccgat caaagggatg atgacggtca tttatgaatt cccggtggat
     5041 tctgaaacta ccctgcaaaa ctacgatatt tacttcacca atgaagagtt aaccgacgag
     5101 caaaaatcgc tgattgagtg gtatcgcgat gtgttccgtc cggaagattt acgtctggtt
     5161 gaaagcgtac agaaagggct gaaatcgcgt ggctatcgtg gtcaggggcg catcatggcc
     5221 gacagtagcg gtagtggcat ttccgaacat ggtatcgccc atttccataa tctgctggcg
     5281 caggtgttta aggactaatg acatcggcgg cggtattttc cgctgctggg ctgatttttg
     5341 atggagtaca gcaatgtcag actatcaaat gtttgaagta caggtgagcc aggttgaacc
     5401 ccttaccgaa caggtgaaac gcttcacgct ggtggcaacc gatggcaaac cgctgcctgc
     5461 gttcaccgga ggaagtcacg tcattgtgca gatgagcgat ggtgataacc agtacagcaa
     5521 tgcgtattca ctactgagtt cgccgcatga cacctcttgt tatcagattg ccgttcggct
     5581 ggaggaaaac tcgcgcggcg gttcccgctt tttgcatcag caggtaaaag tgggcaatcg
     5641 gttaacgatt tcaacgccta ataacctgtt tgcgctaatt ccctcagcca gaaagcatct
     5701 gtttatcgcg ggcggtattg gtatcacccc tttcctgtcg cacatggcag agctgcaaca
     5761 cagcgacgtc gactggcagc tacattactg ctcgcgaaat ccagaaagtt gcgcatttcg
     5821 tgatgagcta gtccagcatc cgcaggctga gaaagttcac ttgcatcact catcaaccgg
     5881 aacacgactg gaattagcgc gattattggc ggatatcgaa cctggcacac acgtttatac
     5941 ctgtggtccc gaggcgctaa atgaagcggt aagaagtgaa gctgcgcgtc tggacatcgc
     6001 cgccgatacg ctgcactttg agcaatttgc tatcgaagac aaaaccggcg atgcctttac
     6061 cctggtgctt gcccgttccg gaaaagagtt tgtggtgccg gaagagatga ctattttgca
     6121 ggttattgaa aataataaag ccgcgaaagt ggaatgttta tgtcgtgaag gggtatgcgg
     6181 aacctgcgaa acagcaatac tggaaggtga agctgaccat cgggatcagt attttagcga
     6241 tgaagagcgt gccagccagc aaagtatgtt gatctgttgt tcgcgtgcga agggtaaacg
     6301 cctggtgttg gatttgtagt ttgccaaagc cggatgtggc gctgagcgcg cccagtccgg
     6361 cttcggaaga ttttactgcg gatattcctg caataaattg tgtaatgctt ccgccatcag
     6421 ctcaccacgc cagccggaaa tcagctccgg caaattgttc tgcggtttca gtttccagtg
     6481 ccagttcagc agttggttga tttgccgacg cgatgccagc aattcggcgc tgatcttatg
     6541 cgtttcgctc acgtcagtaa tcagcgactt aatcgcttta aacgctttac gataaccagg
     6601 catgtccatc aggttaagca tcggctgcgg taaggcctct tccggcaatg tctgcgcttt
     6661 ttccaccagc gtcagcagcg ttttaccgtg aaagcggatt tcgctaccgg ataaacccag
     6721 gctgtccagt tcgcctaaac ttcccggcat ataacgcgcg accgaccaca aatgctcttc
     6781 acgcacgaca aagttcaccg ccagatcacg ctctcgcgcc ttgcgcagtc gccagtcggc
     6841 taacagttgc agacaggcca gttggcgtgt gcgtaactgc caggcattgg tgatatcacg
     6901 ccaggcatct tccggcgcaa cgacttcctg acgacgcatt tgcatcaggc ggcattcatc
     6961 cagcgccgca ggtagccagc cggaggcctc cgtttctacc ataagcttgg cggtgatcgg
     7021 taacagatac cagacatccg ccgctgcgta ttcacactga cgttcggtca gcggtctggc
     7081 cagccagtcg gtgcgcgatt cactcttgtc cagcgtaacg ccggaatact cttccaccat
     7141 ggaagcgaaa ccccatgaca tcgggcgtcc gcagaaggca gaaaggattt gcgtgtcaat
     7201 caagggttgt ggtaattcgc caaagacatt gaggaacact tccagatctt cactgcctgc
     7261 atggagaaat tttgtgatgg acggatcgcg caggatcgct ttcagcggtg accagtcggt
     7321 gatcccgaga ggatcaatta gcgccagatg ctcgccatcg aaaagttgaa tcaaccccag
     7381 ctgcgggtaa taagtgcgcg tacgaacaaa ttcagtatcc agggctatcg ccggaaaggc
     7441 acggacggct tcacacaaag aagccagcgc atcgtccgtg gtaatcattt ggtaattcaa
     7501 attgttttct ctttagtggg cgtcaaaaaa acgccggatt aaccggcgtc tgacgactga
     7561 cttaacgctc aggctttatt gtccactttg ccgcgcgctt cgtcacgtaa ttctcgtcgc
     7621 aaaatttttc cgacgttaga tttcggtaac tcatcacgaa actccaccag cttcggtact
     7681 ttgtatcccg tgagctgacg gcggcaaaaa gtcaccagtg actcttcggt aagcgatgga
     7741 tcttttttca ctacgaagat tttcaccgct tcaccactgg agccggaagg tacgccaaca
     7801 gccgcgactt cctgtacgcc aggatgctgc atgacgacat cttcaatctc gttgggatag
     7861 acgttaaaac cggaaaccag aatcatgtct tttttacgat cgacaatgcg caggaatcct
     7921 tcttcatcca ttaccgcgat gtcgccggtg tgtaaccagc catttttgat gatttcatcg
     7981 gtagcatcgg gacgctgcca gtaacccagc atcacctgcg gtcctttgac acaaagctca
     8041 cccggttgac ctggtggtac ttcattatca tcatcatcca ccagtttggc ttccgtcgac
     8101 ggcaccggca aaccgatgct accactatga taatcaatat catatgggtt aacgctgacc
     8161 agcggcgcac actcggtaag gccatagcct tccagcagat actgtccggt cagtttcacc
     8221 caacgctctg ccaccacttg ctgcactggc atcccaccgc ctgcggaaag atgcagactg
     8281 gagaaatcca gctgctggaa ctctttattg ttcagcaacg cattgaacaa ggtgttaacg
     8341 ccagtgatag cggtaaacgg atatttcgct aactctttta ccaaccctgg aatatcacgc
     8401 gggttagtga taagcaggtt ctgcccaccc agttcgataa acagcaggca gttaatggtc
     8461 agggcaaaaa tgtgatacag cggcagtgcc gttactacca gttctttacc tggatgcagc
     8521 aacggcccat aggtggcgtt gacctgttcc aggttcgcca gcatattgcg gtgagtcagc
     8581 atcgcgcctt tcgccacacc tgtggtgccg ccggtgtatt gcaggaaggc taaatcttcc
     8641 ggcaccagtt cgggtttgac gtactgcatc cggtagccgt tatgcagtgc gctacgaaat
     8701 gaaatggcat ctggcagatg gtatttcggc accaaacgct tgatgtattt aacaacgaaa
     8761 ttgactaccg tgccttttgc cgtagatagc tgatcgccca tacgggtcag aattacgtgc
     8821 tgaacggcgg ttttatcaac cactttttcc agtgtgtgag caaagttaga cacgataaca
     8881 accgccgatg cgccgctatc gttaagctga tgctcaagct cacgcggggt atacaacggg
     8941 ttaacgttta cgacgatcat cccggcacgc aaaatgccaa acagcgccac cggatattgc
     9001 aataaattag gcatcatcaa cgcaacgcga tcgcctttct tcagccccac cccttgttgc
     9061 aaataagcgg caaacgcgcg actgcgttct tccagcttgc ggaaggtcat cacctccccc
     9121 atattcacaa acgcaggctg atcggcgtag cgcgcgaccg actgctcaaa catatctacc
     9181 agagattgat aacggtcagg gttgatctcc gtcggaacgt ccgcgggata acggttaagc
     9241 caaaccttct tcaattcttc acctctaaaa tgcgtgttcg tcgtcatcgc aaccccaaat
     9301 gatatacatg ccgttaacat aatattaact catcatacca gcttgataat tacccaacga
     9361 aaaggttgcg aagcgcgtca ctatttattt ttatctttac cgtaagaatg cagaaacagc
     9421 ggaccagccg ctgtttcttt ttctttaaaa acaagcaatt attcagttac aactgtttgt
     9481 actctcgcgg gaccgggatt ataccagccc catccgccgt agccataggg ccagccacga
     9541 ccgccataaa accatggatc aatcggctga ggcggcataa tcacctgctg ggttaaatgc
     9601 caacgtttgt aacccgttac ttgcatcacc ataaatttat agggcgtatt gccgattttg
     9661 ccgtcaaccg caccagtgat tggcccgact accgtaacca gttgtccacg gaaatccacc
     9721 gggtccagaa aaccgttcac atcggcataa atgcgaccgc gagaaggttc tcccagcgtc
     9781 ggtctggctc cgctgtccag cggtacggta gcaatttcca ggcgggtttt cccttgctgg
     9841 ttttgtaccg caaccacttt gccaccaaag cgtgcctcct gaccaacgta cagctgcggc
     9901 gcactcatca cccgaactaa atcttgttgc ggcgtgggac tgcggccttt aatggcgtcc
     9961 ggcacagtga cacaaccgct cagcattagc gcaaacgtac ctgccagtat gcctttgata
    10021 acattctttt gaaccgccat gatgcgactc ctttttttct cagagcatac tcttaagatt
    10081 cattctttgc ccggaagttt cttccatgcg acgttgttac gtaaataaac cggttcggca
    10141 tgttccaccg ccaccgtttt accctcggca aatatctgac acgcaatcgg cagcatatct
    10201 tccgtcgctg gcagtaaaac ttctccgtcg cgcaaaacca gcccgctctc tttaccgaga
    10261 tccggccagg cttgccagcc cgtccctacc gttacccatt cgccggaaag ctgttgcatt
    10321 cgttcatgaa cgagttcggg tttgagtacg gcttcggttt cttcaccgcg ccagataccg
    10381 ttttcatcac gctgatattc ggcccagtaa acttcgccca tccgcgcatc aatggcagcc
    10441 agcacgcggg ttgcgccgtt tttgcgccac gccccttgcg ccattgtcat tagcgtggag
    10501 acgccaatca tcggcagctc tgcgccaagt gccagccctt gcgcaatgcc aataccaatg
    10561 cgcacgccgg taaagctacc ggggccgcgc ccgtaagcca gagcgttaat atcagtcagg
    10621 gaagttccgc tggtggtcag gatatcctgc accatcggta agattcgttg agtatgttca
    10681 cgagggcaaa gctcaaaatg agcgttgaca gtaccgtcgt tccacagggc gacagagcag
    10741 gcctctgtcg cggtatcgat agccagaatt cgcatgggtc ttcgtgctta gatcaataaa
    10801 aaggcgcgca tcataccata ctccgtaaca aattacctgg aggatggtat cgcaaggaaa
    10861 cgaaccgcac gggcaatgtc acgggtgcgc ggcgcgggcg gcagactggc gagaaacgtc
    10921 gcgccgtaag ggcgcatcac cagccgattg tcacaaatca ccaaaacccc gcgatcgtcg
    10981 gcgtcgcgaa tcagtcgccc taccccctgc ttgagagtaa tcaccgcatc cggcagttgt
    11041 acttcatcga acgggtcgcc accgcgcaaa cggcaatctt ccatgcgcgc ttttaacagc
    11101 gggtcatccg gtgaggtaaa cggcaacttg tcgataatca ccaatgacaa tgtatcgcca
    11161 cgcacgtcca ccccttccca gaaactactg gttgccacaa gaagcgcgtt accggcgctg
    11221 acaaattgct gcaacagttg ccctttgctg gtttccccct gcaacaaaac gggaagcgtc
    11281 atggtagcgc ggaactgctc ggccagatca cgcatcatgg cgtgcgaggt acaaagcata
    11341 aaacaacgac cgttgttagc ttcgatgatc ggtcgcagca ttgccgctaa ctggcgagca
    11401 gaacctggct ggttggtttg cggcagattg cgcggcacac agagtaacgc ctggcggctg
    11461 taatcaaacg ggcttggcaa tagcaacgac tccgcctgtt ctatcccaag ccgcgaggtg
    11521 aaatgatgca gatcgtcgtt caccgacagc gttgctgagg tgaagatcca gctaccaggc
    11581 ttttgcgcca ttaactcttt gaatttatcc gccacgctga gcggcgtgag agccagagta
    11641 aaatggcgcg aagtgcattc gtaccagtag ctgtagcccg gctgattgat ctcttttagc
    11701 cgcttcagcc gtgtgcgata caacgtagcg cgctcaaatg ccgcatccag caaggcggaa
    11761 cgccccagtg acagtttcgc cacgtcataa caaagttcca gggtgtcatc gagcagtaaa
    11821 aatgcccgct gaatttgcgg attagctaac agctcacgca ggttaccgcg ataacctggc
    11881 tcaccgagtt gcagacgaaa atcctgcgca ctctgggcaa gacgatcagc gcacttttgt
    11941 aactgctggg tgtcttttaa ttcggtgcgg taggcgatgg tgatgtcttt tgccaggtcg
    12001 agcagttgtc gactggagag tgactgacca aaatactggc tggcaatgtc cggtagctgg
    12061 tgggcttcgt cgaagatcat gacgtccgct tccgggatca gttcgccaaa tccactctct
    12121 ttaaccacca tatccgccag aaagagatga tggtttacca ccaccacatc ggcgtccatc
    12181 gcttttttac gtgctttgac cacaaagcaa tctttataca tcgggcagtc gctgccaaga
    12241 cagttgtcgt tggtgctggt aaccagcggc cacgcctgtg aatcttccgc cacgctgacg
    12301 caggtgctga tatcaccatc gactgtttga ttagaccagg agcgcagcag gatcacatcg
    12361 cttaagattt gtaccggcag atcgcccccc gccagcgcct gctgttcgag acgttcgagg
    12421 cagaggtagt ttgagcgccc tttcagcagc gccacgttgc ccgtatattt caatgccttt
    12481 gagactgttg gcaaatcgcg gctgtagagc tgatcctgca acgcttttga gccggtcgag
    12541 ataatgactt tctttttcgc ccgcagcgca ggagccaggt aagcgtaggt tttgcccgta
    12601 ccggttcctg cttccaccac cagcggctgg cctttttcta tcgcctgggt gacggctacc
    12661 gccatctgtc gctgtggttc tcgcggctta aagcctggta tcgctttcgc cagctgaccg
    12721 tctggtgcaa aatcgtccgt cacactaccc cctgttgatt tgaacgggga ttatgtcagg
    12781 atgagggcgc tttcgccagt tgaagtggtg acggcaacct cacattgtgg cagtctttgc
    12841 agcacgaaaa tggaagtata atgaggacaa aatgatgact atcgttcgta tcgatgctga
    12901 agcccgctgg tctgatgtag taatccacaa caacacgctc tactacactg gtgtaccgga
    12961 aaacctcgac gccgatgctt ttgagcaaac cgccaacacg ctggcgcaga ttgacgccgt
    13021 gctggaaaaa cagggcagca ataaatcgag cattctggat gccaccattt tcctggctga
    13081 taaaaacgac ttcgcggcga tgaataaagc gtgggatgcc tgggttgtgg cgggtcatgc
    13141 gccggtgcgc tgcacggtgc aagcggggct gatgaacccg aagtataaag ttgaaattaa
    13201 gattgtggct gcggtgtaag ctttatcgaa gcaaaataag tcagacgatc atttatcgat
    13261 aatactggtc ggttttacat aaatcgacca gagattaaga tattactgtt gatgctcctg
    13321 ctaattcatt atttattttt ataatttcaa ttttagctat aaagcta
//
LOCUS       DAJVUJ010000116        13097 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.116, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000116 DAJVUJ010000000
VERSION     DAJVUJ010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13097
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.116"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            7..1635
                     /locus_tag="OQQ48_002985"
     CDS             7..1635
                     /locus_tag="OQQ48_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HCS1953676.1"
                     /translation="MFTAIIAVCILFIIGIIFARLYRRASAEQAFVRTGLGGQKVVMS
                     GGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFFVRVKPSVEGIA
                     TAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENFVQGVQNTVAED
                     LSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLIQETERRRRERNEVEQDVEV
                     AVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNARIAAFEAERRREAEQTRI
                     LAERQIQETEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKSIAIAAKSEQQS
                     QAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETKAVELTVRAKAE
                     KEAAEMQAAAIVKLAEATRKKGLAEAEAQRALNDAINVLSDEQTSLKFKLALLQALPA
                     VIEKSVEPMKSIDGIKIIQVDGLNRGSAAGDANTGNVGGGNLAEQALSAALSYRTQAP
                     LIDSLLNEIGVSGGSLAALTSPLTSTTPVAENVE"
     gene            1874..1933
                     /locus_tag="OQQ48_002986"
     CDS             1874..1933
                     /locus_tag="OQQ48_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCS1953677.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            2250..2309
                     /gene="ibsE"
                     /locus_tag="OQQ48_002987"
     CDS             2250..2309
                     /gene="ibsE"
                     /locus_tag="OQQ48_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HCS1953678.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(2429..3862)
                     /gene="hldE"
                     /locus_tag="OQQ48_002988"
     CDS             complement(2429..3862)
                     /gene="hldE"
                     /locus_tag="OQQ48_002988"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HCS1953679.1"
                     /translation="MKVTLSEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(3910..6750)
                     /gene="glnE"
                     /locus_tag="OQQ48_002989"
     CDS             complement(3910..6750)
                     /gene="glnE"
                     /locus_tag="OQQ48_002989"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HCS1953680.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEKSLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQSPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSHLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSVIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELHQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNDREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFN
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMMLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(6773..8074)
                     /gene="ygiF"
                     /locus_tag="OQQ48_002990"
     CDS             complement(6773..8074)
                     /gene="ygiF"
                     /locus_tag="OQQ48_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HCS1953681.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDDSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAIGLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTKTAMAKLALTEWLVSKAWQPFLDAKAQSKM
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            8316..8936
                     /locus_tag="OQQ48_002991"
     CDS             8316..8936
                     /locus_tag="OQQ48_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HCS1953682.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPTRKRKERWMN"
     gene            9000..10238
                     /gene="cca"
                     /locus_tag="OQQ48_002992"
     CDS             9000..10238
                     /gene="cca"
                     /locus_tag="OQQ48_002992"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HCS1953683.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(10395..11216)
                     /gene="bacA"
                     /locus_tag="OQQ48_002993"
     CDS             complement(10395..11216)
                     /gene="bacA"
                     /locus_tag="OQQ48_002993"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCS1953684.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(11307..11675)
                     /gene="folB"
                     /locus_tag="OQQ48_002994"
     CDS             complement(11307..11675)
                     /gene="folB"
                     /locus_tag="OQQ48_002994"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HCS1953685.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            11780..12397
                     /gene="plsY"
                     /locus_tag="OQQ48_002995"
     CDS             11780..12397
                     /gene="plsY"
                     /locus_tag="OQQ48_002995"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCS1953686.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(12410..>13097)
                     /gene="ttdR"
                     /locus_tag="OQQ48_002996"
                     /gene_synonym="dan"
     CDS             complement(12410..>13097)
                     /gene="ttdR"
                     /locus_tag="OQQ48_002996"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
                     /protein_id="HCS1953687.1"
                     /translation="LVDDVTQIKTRPEGMIRIGCSFGFGRSHIAPAITELMRNYPELQ
                     VHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAHLLTKNKRILCAAPEYLQKYPQPQS
                     LQELSRHDCLVTKERDMTHGIWELGNGQEKKSVKVSGHLSSNSGEIVLQWALEGKGIM
                     LRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYREPLYRSMKLRVCVEFLAAWCQQRL
                     GKPDEGYQVM"
BASE COUNT         3163 a         3683 c         3283 g         2968 t
ORIGIN      
        1 tcctggatgt ttaccgcgat tattgccgta tgcattctgt ttattattgg aattattttc
       61 gccaggctct atcgtcgcgc ttcggcagag caagcttttg ttcgtactgg tttaggtggg
      121 caaaaagtgg tgatgagcgg tggcgcaatc gtgatgccga tctttcatga aataatcccc
      181 atcaatatga atactctgaa gctggaagtc agccgctcaa ccattgatag cctgattacg
      241 aaagatcgta tgcgtgtcga tgtagtagtc gctttctttg tacgggtaaa accgtctgta
      301 gaaggcatcg ccactgcggc gcagacgttg gggcaacgca cgctatcacc agaagactta
      361 cgtatgttgg ttgaagataa atttgtcgat gccctccgtg caacagctgc acaaatgacc
      421 atgcatgagt tacaggatac ccgcgagaac ttcgtgcagg gagtacaaaa tactgtcgca
      481 gaagacctgt cgaaaaacgg tcttgagctg gaaagcgttt cacttaccaa ctttaaccag
      541 acctcgaaag aacatttcaa tccgaacaat gcctttgacg ccgaaggttt aaccaaactg
      601 attcaggaga cggagcgccg tcgtcgcgaa cgtaacgaag ttgaacagga tgtagaagtt
      661 gcggtgcgtg agaaaaaccg tgatgcactt tcgcgcaagt tggagattga acaacaagaa
      721 gcgtttatga cgcttgagca ggagcagcag gttaaaaccc gtactgccga acagaatgca
      781 cgtattgcgg cttttgaagc tgaacgtcgt cgtgaagcag agcagacgcg aattctggct
      841 gaacgacaaa ttcaggaaac ggagatcgag cgcgagcagg ccgtccgctc aagaaaggtt
      901 gaggctgaac gtgaagttcg tattaaagag atcgaacagc agcaggtcac cgaaatcgcc
      961 aaccagacga aatcgatcgc tattgccgcc aaatcggaac aacagtccca ggcagaagcg
     1021 cgtgctaacc ttgcactcgc agaagcagta agtgcgcaac aaaacgtaga aaccactcga
     1081 cagaccgcag aagccgatcg tgctaaacaa gttgccctaa tcgctgccgc gcaggatgca
     1141 gaaaccaaag cggttgaact gaccgtgcgg gcgaaagcag aaaaagaagc cgcagagatg
     1201 caggcggcgg ctatcgttaa gttagccgaa gctacacgta aaaagggtct ggcggaagca
     1261 gaagcacaac gtgcgctgaa cgatgctatc aacgtacttt ctgatgaaca aaccagcctt
     1321 aaattcaaac tggccttgtt gcaggcgcta cctgcggtaa tagaaaaatc cgttgagccg
     1381 atgaaatcta ttgacggtat caagattatt caggtcgatg gtctgaatcg tggcagcgct
     1441 gcgggtgatg caaacacggg taatgtgggg ggcggaaacc tggcggaaca agcattatca
     1501 gccgctctct cttaccgcac acaggcaccg ctgattgact ccttgctcaa tgaaattggc
     1561 gtttcaggcg gctcactggc ggcattgact tcacccttaa cctcaacaac tcccgtcgcc
     1621 gaaaacgtag aataaatttt cagctatgaa cgtccctggc acaaaaagcc ggggattttt
     1681 tatatctgcg ttccgctaaa aactgcaaat gatcaggtcg ttgcaacgtt ttgcgtgacc
     1741 gctcggggaa ggcaaaactg cctctgggaa agcgttgcgc gaggtccggc gctcatcaac
     1801 aatcggggag tcagcaaagg gctgaaacgg gaaagcccct cccgaggaag gggccttaca
     1861 gaaggaaagg gttatgatga agctcgtcat catactgatt gtgttgttac tcgtaagttt
     1921 cgcagcttat taacagccaa tcagaggggg gagaaatcct ccctcaccct tgttccttta
     1981 ctttaggttg aaaaaaaatc agcgtcaaga aggtttcatt aacacatatc cctagcccaa
     2041 aaagccgggg attttttata tctgcgttcc gctaaaaggt gcaaatgctc aggacgttgc
     2101 agcgttttgc gtgaccgctc ggggaaggca aaactgcttc agggaaagcg ttgcgcgggg
     2161 tccggcgctc atcaacaatc ggggagtcag caaggggctg aaacgggaaa gcccctcccg
     2221 aggaaggggc cttatagaag gaaagggtta tgatgaagtt cgtcatcata ctggttgtgt
     2281 tgttactgtt aagtttcccg acttactaac aactgatcag aggggggaga aatcctccct
     2341 tacccttgtt cctttacttt aggttgaaaa aacaacagcg tcaataggcc tgcctgctac
     2401 gaagcgagat ctgtgaaccg ctttccggtt agcctttttt atcctgttgg atcttcttga
     2461 tgatgttagt ggtcgagcaa ccgtcttcga agttgagcac cagcacttcg ccgccgttgg
     2521 cccagacttc tttactcccg gcgatctctt ctggtttata gtcgccgccc ttcaccagta
     2581 aatctggcag gatcccggcg atcaagcgct gcggcgtgtc ttcttcaaac gacaccaccc
     2641 agtcgaccgc ttccagtgcg cccagcacaa tcatacgctg ttcgagcggg ttaacggggc
     2701 gggaatcccc tttcagccgt ttggtggagg catcgctgtt cacagcgaca atcagacggt
     2761 cacccagttt gcgagcattt gccagatagg agacgtgccc ggcatgcagg atgtcaaaga
     2821 caccgttggt cattaccacc ttttcgccgc gtttacgcgc tgccgctacg gccagcttca
     2881 gttcctcttc ggtcatcacg ccaaagccgg tatctgcgcg cccacgcact gcattttcca
     2941 gctcgatcgg cgaaaccgtg gaggttccca gtttgccgac caccacgcca gccgccgcat
     3001 tggcaaagaa gcaggcttct tccagcgaat tacccgctgc cagcgttgcc gccaggacgc
     3061 caatcaccgt gtcgcccgca ccggtaacgt catacacttc ctgcgcctgg gttggcatat
     3121 gcagcggcgc tttacccggt tgcagcagcg acataccctg ttcggaacgg gtcactaaca
     3181 gagccgagag ttcgtaatcg gcaatcagtt tcatgccgcg ctcaacaatc tcttcttcgg
     3241 tcttacattt accgacaaca gcttcaaatt ccgagagatt cggcgttaac agcgtagcgc
     3301 cgcggtagcg ctcaaaatcg gtaccttttg gatcaatcag caccggaacg cccgctttac
     3361 gcgccagttg gatcatctgc tgtacgcttg ccagcgcacc tttggcgtag tcagaaagca
     3421 ccagcgcgcc aatcgaactc agcgcctgat taatccgctc gtgcagcggc tgcggatcaa
     3481 cgccttcgaa accttcttcg aaatccagac ggatcagctg ttggttgcgg gaaagtaccc
     3541 gtaatttggt gatggtcgga tgcgtcggta cagaaacgaa gtcgcatttg acgttgacgt
     3601 cggccagaga tttactcagc gcgcgcgctg catcgtcaat gcccgtcaac ccgaccaggc
     3661 gtgcattagc accgagagaa gcgatattca tcgccacgtt agccgcgccg cccggacgtt
     3721 cttcgatggt attcacttta accacgggca ccggcgcttc cggcgagata cggctggtgg
     3781 gaccgtacca gtaacgatcc agcattacat caccaaccac catcactcct gcacgttcaa
     3841 actctgacag cgttactttc attcctgtct cctgagagat tcaaaatttg cgcgcgataa
     3901 taccatactt cattcttcca ccagccactt ctgccagctt gcccgcacca gatcacgctc
     3961 tgcggtgaag caatcctccg acacatggcc cggcaattcc tgtaatgcca gatgatgaag
     4021 ttcatcgcgc agcgtcgtgt aagcacgggt cagcgccatc gcttcctgct cttccataat
     4081 gtcgttttgc gccagcagct ccagaatacg cacgttgtct gaccagcgcg ttaacttcgg
     4141 tttttcatga gcgtagcgca acaccagata ttgggtaata aattcgatat cggtaattcc
     4201 gccttcatcg gctttgatat caaagcgatt acgatgttta ttgccgagat gagcgcgcat
     4261 tttctcgcgc atttcccgca cttccgtttg cagagtttta ccttcacgcg gcagcatcat
     4321 aatctcgcga cgcactgcgt caaagtgcgc ggtgagctgc ggatcgccgt acactacacg
     4381 cgcacgcacc agcgcctgat gctcccacgt ccaggcctcg tttttctgat aatcagcaaa
     4441 tgcttctgcg gatgtcacca gcattcccgc cgccccggac ggacgcagtc gtgcatccac
     4501 ttcatacaaa atgccggaag aggttcgcgt attgaacaga tgcataatgc gttgcgccag
     4561 acgcagataa aactgtcgcc cgtcgatttc ccgctcgccg tcagtcatcg catccattgg
     4621 gcaatcatgg aggaagatca ggtcaagatc ggaactgtag cctaactccc agccgcccag
     4681 cttgccgtag ccgaccaccg caaaaccacg gccttcgcga tcgttgaggt gatttggctt
     4741 accgtagcgg gccaccattt gaacccacgc ctgctgaacg acggcatcta tcatagcctc
     4801 tgccagccag gttaagtgat cgctcacttt catcaccggt agcgtaccag cgatatccgc
     4861 tgcggcgatg cgtaacagct gcgcctgttt gaactgacgc agcgcctcaa gctgttgctc
     4921 ttcgtcatct tccggcacgc gcagcaaata ctggtgcaac tcatcgcggt aggcatcggt
     4981 cgccgtcggc tggtaaagag tgtttggatc gagcaattca tccagcaata atggataacg
     5041 cgccagctgg ctggcaatca tcggcgatgc ggcacacaga gaaatcaaat gtttaagcgc
     5101 cgcagggaat tcactgagca attctaaata ggtggtgcgg gtaacaatcc ccaccagcaa
     5161 ggcggtaatg cgcgacagcg taacggcagc gtcttcacgc gcacagacat cacttagcag
     5221 atgtggcatc agatggtcga gcacctgacg tcctcgcggc ccgatagtac gcttatccag
     5281 ctctttgcgg aaatcagcaa tcagcgttag gacctgtttg cgatcatcct cgctaagatg
     5341 cgccagcact ggcgtagtgt catcttcctg caacgcatcc tgccacagct cacgccactg
     5401 ttccgacagc gactcttcct gagtttcact ttcgtcgtcg ccaatcaatt cattaaacac
     5461 ccggcgcaca ttggtcatat gtgcggtcag cgccccggtc agttgcggcc agtcagcaaa
     5521 gtccatcgcc cacgccagcc gcgcacggtt aagctcatca gaaggaagcg tttgggtttg
     5581 ttcgtcgtta atgctttgca gcaggttttc cagacgccgc aggaacagat atgccactcg
     5641 caattgttca gcatcgtttt cagaaagcag atgcagcgcg gcaatgacgc tgagcgttgg
     5701 cagtaaagag cgcgactgca gcgacggttc gcgcccaccg cgaatgagct ggaacacctg
     5761 aacgataaat tcaatttcgc gaatgccgcc tgcgccgagt ttgatattgt cggtcaaacc
     5821 acgtcgacgc acttcacggg caatcatccc tttcatgttg cgcagcgact gaatcacgct
     5881 gaaatcgatg taacgacgga aaacaaacgg gcgcagcatc gcacgcaact cgttagcata
     5941 gacgccttcg ctatcgccca taatccgcgc cttgaccatc gcgtaacgct cccagtcgcg
     6001 cccctgctcc tggtaataat cctccaacgc ggcaaagctc agcaccagcg ggccactttc
     6061 gccaaacgga cgcagccgca tatccacgcg atagacgaag ccatcctgcg ttggttgatc
     6121 caacacttta atcagccgct gccccatgcg ggtaaaaaac tgcgcgttat ccagttcccg
     6181 gcgtccgccc tgcgtacaac catgttccgg ccaggcaaaa atcagatcga tatctgagga
     6241 gaaattcagt tccccaccgc ccagcttacc catgcctaaa atcagcagcg gttgcgcttc
     6301 gccctgcgca ttgcacggcg ttccccactc gcggcagcaa gcgtcataca gccagtcacg
     6361 cgcggcgaca atcagcgtct ccgccagatg gctgagctgc tgcaatatgc tctcttcagt
     6421 aaccaaagcc agcgtttgcg cccaggcgat gcgcaccata atgcgccgcc ggaatagccg
     6481 cagctcgcgc attaacccgg cttcgtcact cacattgctg agcgcctcct gcaaccatgc
     6541 cgcgtaatgc tgccattcat cagcctgcgg cgattggctt tccagttccg tcagccactc
     6601 tggatgcgca atcacgctgt cctgcacaaa atcactgaaa gtaagtactg acttcgcctg
     6661 tgcgctaagt gatttctcgg ctaaaggctc tggcagccgc tcaacaacgg tctgccagta
     6721 ctgctgtaac ggtgaagaga gcggcttcat aagcgatttt atccttgcct gattaacgtt
     6781 ttccgctgtg caaccagaac ggttcctgat tgtttgcctc attacggaaa tgttcaattt
     6841 caatgcgttg cccggtcgca atagcgtgac gcagcccctg ccagttctcc agccaggctt
     6901 gcgcgacgac aggatcatag taacccgcca gcaacagtat tgagtcaata tcacgcgtca
     6961 gacgtggcaa ctggtcacgg tagcgatcgc ccaacggctg gcagaaaacg cttttcagtt
     7021 cagcggcatg gcgggaaaga tggatatcgg caaagcgttt gaaggaatcg ctcattttac
     7081 tctgcgcttt ggcatctaaa aacggctgcc aggctttgct taccagccat tcggtcaacg
     7141 ccagcttcgc cattgccgtt ttggtagagt agaccgccgt cacggcagaa accgcagaag
     7201 cgatggtcgc ttcgcattga gtcagcaaat cgcgtaagtg agtgctcgct ttgcgcggca
     7261 caataccacc gaacagcatc agtgtatgac ggaccagacc aatggctgcc agcacctgtt
     7321 ctttcgccgc atcgttgccg cgtacccaca gttcttcatg atactgccat tgcgctaacg
     7381 ccagctcgag cgccgcttcc agcccctgtt ccacatcggc ttttgccgca acatgcaaaa
     7441 tggtggtcgg tttgatttca cgcgccggat tgccctgcgc cagatgatag ccacgcgccg
     7501 ctttgctcag gctgccctgg cgtaatccgg tttgcgatac cagttggttc gccagtttca
     7561 gcaccgcgcg cgtgtcgccg ctaagcagtt ccagttccag ctcacagata ggttcagcaa
     7621 attcccccgc tttcacctcc ccctgatcga gagcgatttc aattcggctg tcatcgaccg
     7681 ccaccagcca tttttcgcga taaaaatcgg tgctgaacag cggttgtacg cgggaagcga
     7741 gatcggcggg caattcgccg ttcggccaga cttccgtcgg taactgcgcc aggtcgagcg
     7801 tcggttcgct caacgccacg ttatattccg ggcgctggtg taagccgcct gtcactcttc
     7861 ctgcaacttt catggtcatc tcatagcgac cgttttcgcc acgaatacgt aagcccatat
     7921 cgtgcccacg cagccagtta tccggcgttt cgtagtaaat attcagcaac tgcacggggt
     7981 catggtgctc gccgcccagc gtattgagat ggtcacgcaa cgcctcaacg gcactgtgat
     8041 taacaataaa ctttaattcg atttcctgag ccatggcctt tacttatcgg ttatgtcaca
     8101 taagtgacga tgaacggcga acttaatgcg atcttttttg tcagtagata gtattttgcg
     8161 ccaaattgcc atgcaacggg caatttgacg ggcgtaaaag tttgaagcag tggcaagacc
     8221 tgacacagag gattccgatt gatgtcgaat gctttgcgta gcgacactga tgccactact
     8281 atcgttccac tttccatgac aataacgaca gcctgatgcc aaaattacgc ctgatcggat
     8341 taactttact cgcacttagc gccactgccg tctcacacgc tgaagaaacg cgctatgttt
     8401 ctgatgaact gaatacctgg gtccgtagcg gtccgggaga tcattatcgc ctcgtgggca
     8461 cggttaacgc cggcgaggaa gtgaccttat tacaaactga cgccaacacc aattatgccc
     8521 aggtgaaaga cagctctggc cgtaccgcct ggatcccgtt gaaacaactt agcactgagc
     8581 caagcctgcg ctcccgtgtg ccagatctgg aaaatcaggt caaaaccctg accgataaac
     8641 tcaccaatat cgataacacc tggaatcagc gcaccgcaga aatgcagcaa aaagtggcgc
     8701 agagcgacag cgtgatcaac ggattaaaag aagaaaatca gaaattgaaa aacgagctga
     8761 ttgtcgcgca gaaaaaggtc gatgccgcca gcgtacagct ggatgacaaa cagcgcacca
     8821 tcatcatgca atggtttatg tatggcggtg gcgtgctggg gcttggcttg ctgctcggtc
     8881 tggtactgcc acacctgatc ccaacgcgca aacgcaaaga gcgctggatg aactaaatcg
     8941 ccttcttcgt tgcactgtct tattattagg aattgtgtat ttgacgaagg gatgatggcg
     9001 tgaagattta tctggtcggt ggtgctgttc gggatgcatt gttagggcta ccggtcaaag
     9061 acagagattg ggtggtggtc ggcagtacgc cacaggagat gctcgacgcg ggctaccagc
     9121 aggtaggccg cgattttcct gtgtttctgc atccgcaaac gcatgaagag tatgcgctgg
     9181 cacgtaccga acggaaatcc ggttccggtt acaccggttt tacttgctat gccgcaccgg
     9241 atgtcacgct ggaagatgat cttaagcgtc gcgatctgac cattaatgcg ctggcccagg
     9301 acgataacgg cgagattatc gacccgtaca acggtctggg cgatctgcaa aatcgtctgt
     9361 tgcgccatgt ttcccccgct tttggcgaag atccgttacg cgttctgcgc gtggcgcgtt
     9421 ttgctgcgcg ttatgcccac ctcggttttc gtattgccga tgaaactctg acgctgatgc
     9481 gcgagatgac ccatgcgggt gaactggaac acctgacgcc tgaacgggta tggaaagaga
     9541 cggaaagcgc cctcaccacc cgcaatcccc aggtgttctt ccaggtactg cgcgattgcg
     9601 gtgcacttcg cgttttattc ccggaaattg acgccctgtt tggcgttccg gcccctgcca
     9661 ggtggcatcc ggaaatcgac accggtattc ataccttaat gacgctctca atggcggcga
     9721 tgctgagtcc gcaggttgat gtccgtttcg caactttatg ccacgatctc ggtaaaggac
     9781 tgacgccgcc agaactttgg ccgcgtcatc atgggcatgg cccggcgggt gttaagttag
     9841 tggaacaact atgccagcgt ctgcgcgtgc caaatgaaat tcgcgattta gccagactgg
     9901 tggctgagtt tcacgatctc atccacacct tcccaatgct gaacccaaaa accatcgtca
     9961 aattatttga ttccatcgat gcctggcgta aaccgcagcg tgtcgagcaa ctggcgctga
    10021 ccagcgaggc tgacgtacgc ggcagaaccg gttttgaatc agcggactac ccgcaaggcc
    10081 gttggttgcg cgaagcctgg gaagtggcgc agtcagtgcc gacaaaagcg gtcgttgaag
    10141 cgggatttaa aggtgtggag attcgcgagg agctgacccg acggcggatt gcggcggtag
    10201 ccagttggaa ggaacaacgt tgcccaaagc ctgactgagt ataaaactaa acgttaacac
    10261 gaaccatccc ctcgcccctt cggggagagg gtgaggggaa cagaccagca ccggtgcgaa
    10321 caatcacccc caccccggcc ctctccctac agagcgaggg agaaaaccag gcctgctccc
    10381 gaaccttttc tcaatcaaaa gaacacgacg tacaccgcag ccgccacaat aaagcgataa
    10441 atggcgaacg ggataaacga aatgcgctta atcaattgca ggaaggtttt aatcgctatc
    10501 agcgccacca caaaagcggt gataaaccca acggcaaaca tcgggatatc gccggttgtc
    10561 aggaagcccc agcttttgta gagatcgagc gccgttgcgc ccatcatcat cggtaccgcc
    10621 agcaggaacg agaactcgga agcagcgtaa cggctcacgc ccatcagcat cccacctgaa
    10681 atggtggccc cggaacggga gaaacccggc cacagcgcca gacactggaa acagccaatc
    10741 ataaacgcct gacgataggt catatcatca agacccggcg cacgcggctc tttcggcttc
    10801 aggcattcgg cggcaatcag caacaaaccg ccaacgacca gtgcatacat cacatttatc
    10861 gggttaaaca atgacttaat cgtgtcgtgg aacaacagcc ccagtaccac cgccggaatc
    10921 atccccagca aaatgtggat cagcgttaaa cgacctttgc tttctccttc gtgctgcaac
    10981 gggcggccaa agtggatgcc aatcaggcca aacagacgcc gccagaacat cactactacc
    11041 gccagaattg atcctaactg gatcacaact tcaaaggttt tcgccgtgtc gccctcgaac
    11101 cccaacaagt gaccgacaat aatcatatgg cccgtgctgg ataccggcag aaattctgtc
    11161 aatccttcga ccacacccaa tattgccgct atcagcagcg agtgcatatc gctcatcaat
    11221 aaacccctaa attattaaaa tgtaccgctt gtccggacta ctgcagtaag accaggttat
    11281 aaccgtttgg tttaacagct gtaaaattaa ttattttctt tcagattatt gccacgctca
    11341 atgattacac caacattcgc cgcccgcgcc actgcgcctg gcttgctgag tttgatgcgc
    11401 acccacggcg agttgaagcg tgctaacagc agctccgcca cctcttcagc cacgcgttcc
    11461 accagcgcaa aacgcgcccc ctcgacgtgg ctgaccaccg tttctgcaat gtcagcgtaa
    11521 ctgaggcaat ccgccacatc atcacttttc gccgctttac ggttatccca tgccatttcg
    11581 atatcgaaca ctaacttctg ttcgatggtc tgttcccagt cgtaaacacc aatagtggtg
    11641 attaccgaaa gttgctctat aaatacaata tccatcacgt cctgcctgct ttttggctaa
    11701 ccggatacca cttccggcga aatgtgcgta ttatccacag attcatcgtt gaacacgaat
    11761 tttcaaaacg gaacagctta tgagtgcaat cgcgcctgga atgatcctca tcgcgtacct
    11821 ctgcggctcc atttccagtg ccattctggt ttgccgcttg tgtgggctgc ccgatccgcg
    11881 aaccagcggc tccggcaatc caggcgcaac caatgtgtta cgtatcggtg gcaagggagc
    11941 agccgtagca gtactgattt tcgacgttct gaaaggaatg ttgcccgtct ggggcgcgta
    12001 tgaattaggt gtcagcccct tctggctagg cttaattgcc atcgccgcct gtcttggaca
    12061 catctggccc gttttcttcg gatttaaagg aggaaaaggc gttgctaccg cttttggtgc
    12121 catcgcaccc attggctggg atctcaccgg agtaatggcg ggaacctggt tactgaccgt
    12181 gctattgagc ggatactcgt cgctgggagc gattgtcagt gcactgattg ctccgtttta
    12241 tgtctggtgg tttaagccac aattcacctt cccagtttcg atgctctctt gcctgatcct
    12301 gctgcgtcat catgacaaca tccagcgtct gtggcgtcgt caggagacaa aaatctggac
    12361 gaaattcaaa agaaagcgcg aaaaggatcc cgagtgattt ctggtggatc tacatgacct
    12421 gatagccttc atcgggcttg cccagccgtt gctggcacca tgccgccaga aattccacgc
    12481 agacacgtaa tttcatgctg cgatagagcg gctcccggta aacagcccag atattggcgc
    12541 tttgtgcata ctccggcaat acttgcacca gtttgccact ctccagaaac ggcaacacat
    12601 cccactcgga acgcagcata atccctttgc cctccagcgc ccattgcaac actatctcgc
    12661 cgctattgga ggaaagatgc ccgctaacct tcaccgattt tttctcctga ccgttcccca
    12721 actcccatat tccgtgggtc atatcgcgct ctttggtcac cagacagtca tgacgactta
    12781 attcctgtaa tgattgtggc tgcggatatt tttgcagata ctcaggtgct gcgcataata
    12841 ttcttttatt tttcgttaac agatgcgcaa tataataatc aggaatttcg tcattaatac
    12901 gaatatccag atcaatatta tcctgcacta aatcaatttg ccgatcgaac aattcaaaat
    12961 gcacctgtaa ctcaggataa ttgcgcataa gttcggtaat ggctggcgca atatggctgc
    13021 gcccaaaacc aaaactgcaa ccaatacgaa tcatcccctc cgggcgcgtt ttgatttgcg
    13081 tgacgtcatc gaccagt
//
LOCUS       DAJVUJ010000117        13097 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.117, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000117 DAJVUJ010000000
VERSION     DAJVUJ010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13097
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.117"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            176..1150
                     /gene="cysB"
                     /locus_tag="OQQ48_002997"
     CDS             176..1150
                     /gene="cysB"
                     /locus_tag="OQQ48_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HCS1953688.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            1445..1609
                     /locus_tag="OQQ48_002998"
     CDS             1445..1609
                     /locus_tag="OQQ48_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HCS1953689.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
     gene            1612..1779
                     /locus_tag="OQQ48_002999"
     CDS             1612..1779
                     /locus_tag="OQQ48_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1953690.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            1893..1988
                     /locus_tag="OQQ48_003000"
     CDS             1893..1988
                     /locus_tag="OQQ48_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1953691.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            2152..4827
                     /gene="acnA"
                     /locus_tag="OQQ48_003001"
                     /pseudo
     CDS             2152..4827
                     /gene="acnA"
                     /locus_tag="OQQ48_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
     gene            complement(4891..5481)
                     /gene="ribA"
                     /locus_tag="OQQ48_003002"
     CDS             complement(4891..5481)
                     /gene="ribA"
                     /locus_tag="OQQ48_003002"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HCS1953692.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            5651..6415
                     /gene="pgpB"
                     /locus_tag="OQQ48_003003"
     CDS             5651..6415
                     /gene="pgpB"
                     /locus_tag="OQQ48_003003"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HCS1953693.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLLWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            6564..6872
                     /gene="lapA"
                     /locus_tag="OQQ48_003004"
     CDS             6564..6872
                     /gene="lapA"
                     /locus_tag="OQQ48_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HCS1953694.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            6879..8048
                     /gene="lapB"
                     /locus_tag="OQQ48_003005"
     CDS             6879..8048
                     /gene="lapB"
                     /locus_tag="OQQ48_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HCS1953695.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNRARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            8241..8978
                     /gene="pyrF"
                     /locus_tag="OQQ48_003006"
     CDS             8241..8978
                     /gene="pyrF"
                     /locus_tag="OQQ48_003006"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HCS1953696.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSDAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            8978..9304
                     /gene="yciH"
                     /locus_tag="OQQ48_003007"
     CDS             8978..9304
                     /gene="yciH"
                     /locus_tag="OQQ48_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HCS1953697.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(9430..9648)
                     /gene="osmB"
                     /locus_tag="OQQ48_003008"
     CDS             complement(9430..9648)
                     /gene="osmB"
                     /locus_tag="OQQ48_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HCS1953698.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(9917..10666)
                     /locus_tag="OQQ48_003009"
     CDS             complement(9917..10666)
                     /locus_tag="OQQ48_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCS1953699.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEVIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            complement(10756..10929)
                     /locus_tag="OQQ48_003010"
     CDS             complement(10756..10929)
                     /locus_tag="OQQ48_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1953700.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            complement(11076..13061)
                     /gene="pdeR"
                     /locus_tag="OQQ48_003011"
     CDS             complement(11076..13061)
                     /gene="pdeR"
                     /locus_tag="OQQ48_003011"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HCS1953701.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDVLRFSTTETTEPDRTL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWAGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLKEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTCKGQRLFLFRNKFVHSGSGKSEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQDLIDHAINHADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMA
                     SRILTRLRLPFRIGLIEVYTSCSVGIALSPEHGSDSTAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWRDKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSQLGAQVHLDDFGTGY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
BASE COUNT         3333 a         3084 c         3294 g         3386 t
ORIGIN      
        1 tcgcgaaagc ggcccttttt tattgcatat tattttttct tcacacctat acactaaggc
       61 tataaatgat atagtggtta tagttagcac cttttttatt attaaatcgt attagtcacc
      121 cgccaggtgt gacgaaaaaa cgatgttctg atggcgtcta agtggatggt ttaacatgaa
      181 attacaacaa cttcgctata ttgttgaggt ggtcaatcat aacctgaatg tctcatcaac
      241 agcggaagga ctttacacat cacaacccgg gatcagtaaa caagtcagaa tgctggaaga
      301 cgagctaggc attcaaattt tttcccgaag cggcaagcac ctgacgcagg taacgccagc
      361 agggcaagaa ataattcgta tcgctcgcga agtcctgtcg aaagtcgatg ccataaaatc
      421 ggttgccgga gagcacacct ggccggataa aggctcactg tatatcgcca ccacgcatac
      481 ccaggcacgc tacgcattac caaacgtcat caaaggcttt attgagcgtt atcctcgcgt
      541 ttctttgcat atgcaccagg gctcgccgac acaaattgct gatgccgtct ctaaaggcaa
      601 tgccgatttc gctatcgcca cagaagcgct gcatctgtat gaagatttag tgatgttacc
      661 gtgctaccac tggaatcggg ctattgtagt cactccggat cacccgctgg caggcaaaaa
      721 agccattacc attgaagaac tggcgcaata tccgttggtg acatatacct tcggctttac
      781 cggacgttca gaactggata ctgcctttaa ccgcgcaggg ttaacgccgc gtatcgtttt
      841 cacggcaacg gatgctgacg tcattaaaac ttacgtccgg ttagggctgg gggtaggggt
      901 cattgccagc atggcggtgg atccggtcgc cgatccggac cttgtgcgcg ttgatgctca
      961 cgatatcttc agccacagta caaccaaaat tggttttcgc cgtagtactt ttttgcgcag
     1021 ttatatgtat gatttcattc agcgttttgc accgcattta acgcgtgatg tcgttgatgc
     1081 ggctgtcgca ttgcgctcta atgaagaaat tgaggtcatg tttaaagata taaaactgcc
     1141 ggaaaaataa tttcttgcgt tattttcggc accttttatg tagcgaaggt gccggaatat
     1201 attctctttt gttacttatt tttataccgt gcacaatatt tttatccctc atatttatag
     1261 ggtaaattac ctgtaaagta gtgtctaatt acccgcctcg caaattccag ttttcaaata
     1321 tctatttcag gtcaaaagat tgaattattc actggagacg attcgtaaat tcgctggatt
     1381 tttgactaaa gtttctttag gatttgtctc atcgtatgat taattacacc aatcgttcaa
     1441 cgtcttgctg ataagtgatg gcgattatat gaggttagca atgccttctg gaaatcagga
     1501 accccgccgc gatcctgaat taaaacgtaa agcctggctg gcggtttttc ttggttctgc
     1561 acttttctgg gtggttgtcg cactgctgat ttggaaagtg tggggataac tatggtcggt
     1621 caggagcaac tggagtcgtc accattatgc cagcatagtg acaatgaaac agaaacgaaa
     1681 cgggaatgtt ccgtcgttat tccagacgac tggcaactaa catcgcagca gcaagccttt
     1741 atagaactgt ttgctgaaga tgatcagccg aaacaataat tatcatcatt cttattaccc
     1801 atttttaatg aattaaaggg cttttaatac accgcagcaa taacagcttg agttatctca
     1861 acacaaaata ataaccgtta agggtgtagc ctatgatcaa cacaaatatg aaatattggt
     1921 cctggatggg cgcgttttct ctgtcgatgc tcttctgggc cgaactcctc tggatcatta
     1981 ctcactgatc cttgaccccg ctgcggcggg gttgtcattt gctttgccac aaggtttctc
     2041 ctcttttatc aatttgggtt gttatcaaat cgttacgcga tgtttgtgtt atctttaata
     2101 ttcaccctga agagaatcag ggcttcgcaa ccctgtcatt aaggaggagc tatgtcgtca
     2161 accctacgag aagccagtaa ggacacgttg caggccaaag ataaaactta ccactactac
     2221 agcctgccgc ttgctgctaa atcactgggc gatatcaccc gtctacccaa gtcactcaaa
     2281 gttttgctcg aaaacctgct gcgctggcag gatggtaact cggttaccga agaggatatc
     2341 cacgcgctgg caggatggct gaaaaatgcc catgctgacc gtgaaattgc ctaccgcccg
     2401 gcaagggtgc tgatgtagga ctttaccggc gtacctgccg ttgttgatct ggcggcaatg
     2461 cgcgaagcgg ttaaacgcct cggcggcgat actgcaaagg ttaacccgct ctcaccggtc
     2521 gacctggtca ttgaccactc ggtgaccgtc gatcgttttg gtgatgatga ggcatttgaa
     2581 gaaaacgtac gcctggaaat ggagcgcaac cacgaacgtt atgtgttcct gaaatgggga
     2641 aagcaagcgt tcagtcggtt tagcgtcgtg ccgccaggca caggcatttg ccatcaggtt
     2701 aacctcgaat atctcggcaa agcagtgtgg agtgaattgc aggacggtga atggattgct
     2761 tatccggata cactcgttgg tactgactcg cacaccacta tgatcaacgg ccttggcgtg
     2821 ctggggtggg gcgtgggtgg gatcgaagca gaagccgcaa tgttaggcca gccggtttcc
     2881 atgcttatcc cggacgtagt gggctttaaa cttaccggta aattacgtga aggtattacc
     2941 gccacagacc tggttctcac tgttacccaa atgctgcgca aacatggcgt ggtggggaaa
     3001 ttcgtcgaat tttatggtga tggtctggat tcactaccgc tggcggatcg cgccaccatt
     3061 gccaatatgt cgccagaata tggtgccacc tgtggcttct tcccaatcga tgctgtaacc
     3121 ctcgattaca tgcgtttaag cgggcgcagc gaagatcagg ttgagttggt cgaaaaatat
     3181 gccaaagcgc agggcatgtg gcgtaacccg ggcgatgaac cgatttttac cagtacgtta
     3241 gaactggata tgaatgacgt tgaagcgagc ctggcagggc caaaacgccc acaggatcgc
     3301 gttgcactgc ccgatgtacc aaaagcattt gccgccagta acgaactgga agtgaatgcc
     3361 acgcataaag atcgccagcc ggtcgattat gttatgaacg gacatcagta tcagttacct
     3421 gatggcgctg tggtcattgc tgcgataacc tcgtgcacca acacctctaa cccaagtgtg
     3481 ctgatggccg caggcttgct ggcgaaaaaa gccgtaactc tgggcctcaa gcggcaaccg
     3541 tgggtcaaag cgtcgctggc accgggttcg aaagtcgttt ctgattatct ggcaaaagcg
     3601 aaactgacac cgtatctcga cgaactgggg tttaaccttg tgggatacgg ttgtaccacc
     3661 tgtattggta actctgggcc gctgcccgat cctatcgaaa cggcaatcaa aaaaggcgat
     3721 ttaaccgtcg gtgcggtgct gtccggcaac cgtaactttg aaggccgtat ccatccgctg
     3781 gttaaaacta actggctggc ctcgccgccg ctggtggttg cctatgcgct ggcgggaaat
     3841 atgaatatca acctggctgc tgagcctatt ggtcatgatc gtaaaggcga cccggtttat
     3901 ctgaaagata tctggccatc ggcacaggaa attgcccgtg cggtagaaca agtctccaca
     3961 gaaatgttcc gtaaagagta cgcagaagtt tttgaaggca cagcagagtg gaaggaaatt
     4021 aacgtcacac gatccgatac ctacggttgg caggaggatt caacctatat tcgcttatcg
     4081 cctttctttg atgaaatgca ggcaacacca gcacctgtgg aagatattca cggtgcgcgg
     4141 atcctcgcaa tgctggggga ttcagtcacc actgaccata tctctccggc gggcagtatt
     4201 aagcccgaca gcccggcggg tcgatatcta caaggtcggg gtgttgagcg aaaagacttt
     4261 aactcctacg gttcgcggcg tggtaaccat gaagtgatga tgcgcggcac cttcgccaat
     4321 attcgcatcc gtaatgaaat ggtgcctggc gttgaagggg ggatgacgcg gcatttacct
     4381 gacagcgatg ttgtctctat ctatgatgcc gcgatgcgct ataagcagga gcaaacgccg
     4441 ctggcggtga ttgccgggaa agagtatgga tcaggctcca gccgtgactg gacggcaaaa
     4501 ggtccgcgtc tgcttggtat tcgtgtggtg attgccgaat cgtttgaacg aattcaccgt
     4561 tcgaatttaa ttggcatggg catcttgccg ctggaatttc cgcaaggcgt gacgcgtaaa
     4621 acgttagggc taaccgggga agagaagatt gatattggcg acctgcaaaa tctacaaccc
     4681 ggcgcgacgg ttccggtgac gcttacgcgc gcggatggta gccaggaagt cgtaccctgc
     4741 cgttgtcgta tcgacaccgc gacggagttg acctactacc agaacgacgg cattttgcat
     4801 tatgtcattc gtaatatgct gaagtaacaa gtatttgctt gccggttatt ttgcttccgg
     4861 caagcaaatt aattccacaa tgcgagaggg ttatttgttc agcaaatgac ccattttctc
     4921 ggctttggta tcgagataat gttcgttatt ggggttacga cctacaatca atggtacgcg
     4981 ttcaacaata ttaatccctg cttcggtcag aatttcgact tttttcgggt tattggttaa
     5041 caagcggact tcattgacgc caaggagttt gaacatatca gcgcaaagag tgaagtcgcg
     5101 ctcatcagcg gcgaagccta actggtggtt agcctctacg gtatcgtaac cttgatcctg
     5161 cagtgcgtaa gcgcggattt tattcagcag accaatgtta cgaccttcct gacggtgata
     5221 cagcaaaata ccacggcctt cctcggcaat ttgcgtcaat gccgcttcga gctggaagcc
     5281 acaatcgcag cgcaagctga acagggcgtc accggtcaga cattcggaat ggacgcgcgc
     5341 aagtaccggg gtatgcccgg aaatatcgcc atagactagc gcgacatgat cgtgtccggt
     5401 tgccagttct tcaaatccca ccatcaggaa atcgccccat ggggttggca gtttggcttc
     5461 tgccacacgt ttaagctgca tgaaattctc cagataatgc tggttctgta ttggcttatt
     5521 ttgccataac gagaaggggt tcacctaatc acgacgcgtc gatcgttcac ggaatggcac
     5581 aaatctgtca atttttcctg gaactggcgt tttcagttat gattgtggga cttatcaaaa
     5641 aggagaggcc atgcgttcga ttgccagacg taccgcagtg ggagctgcac tattgcttgt
     5701 catgccagta gccgtatgga tttctggctg gcgttggcaa cctggagaac aaagttggct
     5761 actaaaagcg gctttttggg ttactgaaac tgtcacccag ccctggggcg tcattacaca
     5821 tttgatttta ttcggctggt ttctctggtg tctgcgtttt cgcattaagg ctgcctttgt
     5881 attatttgcc attctggcgg ccgcaatcct tgtgggacaa ggcgttaaat cctggatcaa
     5941 agacaaagtc caggaaccac gaccttttgt tatctggctg gaaaaaacac atcatattcc
     6001 ggttgatgag ttctacactt taaagcgagc agaacgcgga aatctagtga aagaacagtt
     6061 ggctgaagag aaaaatatcc cacaatattt gcgttcacac tggcagaaag agacggggtt
     6121 tgcctttcct tccggtcaca cgatgtttgc tgccagttgg gcactgctgg ccgttggttt
     6181 gctgtggccg cgtcggcgaa cgttaaccat tgctatcttg ctgctctggg caacgggagt
     6241 catgggaagc cgcctgctgc tcgggatgca ttggccacgc gatctggtag tagctacatt
     6301 gatttcgtgg gcgctggtgg cggtggcaac gtggcttgcg caacgaattt gtgggccatt
     6361 aacaccacct gcggaagaaa atcgcgaaat agcgcaacga gaacaagaaa gttaacgctg
     6421 gttgattttc cgaatttagc ccttaaatca tcaacaatgc gcgtggatgc catttcgcag
     6481 acggcgcgaa aatggtactt taaagggcta ttgcggtaag ttgaccataa tttattcgct
     6541 ctaaccacat aacgggaagt aatgtgaaat atttactcat tttcttactg gtgttagcga
     6601 tcttcgtgat ttcggtcacg ttgggtgcgc agaacgatca acaggtgacg tttaattatc
     6661 tgttagcgca aggggagtac cgtatttcca cattgctggc ggtattgttt gctgcggggt
     6721 ttgctatcgg ttggttgatt tgtggcctgt tctggctgcg agttcgtgtt tccctggcgc
     6781 gcgctgaacg taaaataaag cgactggaaa accagctttc acccgcgact gacgtggctg
     6841 tagtgccgca ctcgtcagcg gcgaaggaat aactttctat gctggagttg ttgtttctgc
     6901 ttttgcctgt agccgctgcc tatggctggt atatgggccg cagaagtgcg caacaaaaca
     6961 agcaagatga agccaaccgc ttgtcgcgtg attacgtagc gggggttaac ttcctgctta
     7021 gtaatcaaca ggataaagcg gtagacctgt ttctcgatat gcttaaagag gatacaggca
     7081 ccgttgaagc ccaccttacg ctcggaaacc tgttccgttc gcgtggcgaa gttgatcgcg
     7141 ctattcgcat ccatcagacc ctaatggaaa gcgcctcgct gacctatgaa cagcgtctgt
     7201 tggcgattca acaactaggg cgtgattaca tggccgccgg gttatacgac cgcgcggaag
     7261 acatgttcaa tcagctgacc gatgaaactg acttccgcat tggcgctctg caacagttgc
     7321 tacaaatcta ccaggctacc agtgagtggc agaaagcaat tgatgttgcc gaacgcctgg
     7381 tgaagctggg taaagataaa cagcgcgtcg aaattgccca tttctactgt gagttagccc
     7441 tgcagcatat ggccagcgac gatctcgatc gtgccatgac cttgctaaaa aaaggggcgg
     7501 cggcagataa aaacagagcc cgcgtatcca tcatgatggg acgcgtgttt atggcgaaag
     7561 gagaatacgc caaagccgtc gaaagtttgc aacgcgtgat atcccaggac agagaactgg
     7621 tcagcgaaac gctggaaatg ttgcaaacct gctatcagca gttgggtaaa actgccgaat
     7681 gggcagaatt cctgcaacgc gcggtggaag agaacaccgg tgccgatgct gaattgatgc
     7741 ttgctgatat catcgaagcg cgcgacggta gtgaggccgc acaggtctat attacgcgcc
     7801 agcttcagcg tcatccgacc atgcgtgtgt tccataagtt aatggattac cacttaaatg
     7861 aagcggaaga agggcgtgcc aaagagagcc tgatggtgct gcgtgacatg gttggcgaaa
     7921 aggtacgtag taagcctcgt tatcgctgcc agaaatgtgg ttttaccgca tacaccctct
     7981 actggcattg tccgtcttgt cgggcctggt caaccattaa accgattcgc ggtcttgatg
     8041 gcctgtaatt tttaaaaaaa tccgacttta gttacaacat gctagttatt gatgttccat
     8101 tgtgctccgg caacgacgga gcagaaaaag cctgccaggg gaaaaatcgc aactgttaat
     8161 tttttatttc cacgggtaga atgctcgccg tttacctgtt tcgcgccact tccggtgccc
     8221 atcatcaaga aggtctggtc atgacgttaa ctgcttcatc ttcttcccgc gctgttacga
     8281 attctcctgt ggttgttgcc cttgattatc ataatcgtga tgacgcgctg tcctttgtcg
     8341 acaagatcga cccacgcgat tgtcgtctga aggtcggcaa agagatgttt acattgtttg
     8401 ggccacagtt tgtgcgcgaa cttcaacagc gtggttttga tatctttctt gacctgaaat
     8461 tccacgatat tcctaacact gcagcgcacg ctgtcgctgc tgcagctgac ttaggcgtgt
     8521 ggatggtgaa tgttcatgcc tctggtgggg cgcgtatgat gaccgcagcg cgtgaggcac
     8581 tggttccgtt tggcaaagat gcaccgcttt tgattgctgt aacagtgttg accagcatgg
     8641 aagctagcga cctggccgat cttggcgtga cactgtcacc tgcagattat gcagaacgtc
     8701 tggcggcact gacgcaaaaa tgtggccttg atggtgtggt gtgttctgct caggaagctg
     8761 tgcgctttaa acaggtattc ggtcaggagt tcaaactggt tacaccgggc attcgtccgc
     8821 aggggagtga tgctggtgac cagcgccgca ttatgacgcc agaacaggcg ttggcggctg
     8881 gtgttgatta tatggtgatt ggtcgcccgg taacgcaatc ggtagatcca gcgcaaacac
     8941 tgaaagcgat caacgcctct ttacagcgga gtgcatgatg agtgattcca acagccgtct
     9001 ggtctactca acggaaaccg gacgtattga tgagccaaaa gcggccccag tacgccctaa
     9061 aggcgatggt gtggtgcgta ttcagcgtca gaccagtgga cgtaaaggta agggcgtttg
     9121 cctgattacc ggtatcgatc tcgatgatgc cgaactgaca aaacttgcag cggaactgaa
     9181 gaaaaaatgc ggctgcggcg gagcagttaa agatggagtt attgaaatcc agggcgataa
     9241 gcgtgattta ttaaaatcac tacttgaagc gaaagggatg aaggtaaaac tcgcaggcgg
     9301 ttaacataaa aagccacgga tatatccgtg gctttcgaat attttactgt gtgtattatt
     9361 cagttatttc gtactgaaca aaatcaagcg tattataatt gcgagtcgat aatcgacatt
     9421 tatttacgat tatttaccga cctggtgacc aataacacca ccgacagctg caccacctaa
     9481 tgtacccaac gtactgccat cggtcagtac tgcaccgcct aatgcccctg cacccgcgcc
     9541 gattgcggtg ttgcggtccc gtttagacca gttagaacag gcactcagag acattgccaa
     9601 agtaattgcc agaacagccg cggtcatttt tttgctcgtt acaaacataa tactctctcc
     9661 tgaatttatg attcacggaa gtaagctctc tataactata atagctaaga ataagcctgg
     9721 tgaaattatt ccgtgaaatc tgctcgcgcg cagggttata tcacgcaggt gataatgact
     9781 tcctgttata tcgctgataa taattttata tcttaagcgt gttaataaca ggtaaatagt
     9841 cttaattatc aaccaggaat catcttagag cggatgattt gccaaactgc aaatcatccg
     9901 tagagaaggg aaatggttaa atgtcaacga cgtgaacggt gagtttactg tgctcaagat
     9961 gcatcagatc gctggcgcgt attttcgaat cggtaatcac gcgattgaat cgctcaacgg
    10021 gaccgatgga gtatgaatgt acagcaccaa atttcgagct gtcagtcagg actatcactt
    10081 cgcactcttt ttccagcacg gcattgacca catcggtacg catcatatcg cgaccggtga
    10141 atccagtttc aggttgccag ccatcaatac caataaatgc tttgctgaaa tgcacctgct
    10201 ggatgcactg gcgtgtcaac gggccaacca tactttcgct ttttttctgg tacacgccac
    10261 cgagcaaaat gacttcacaa ggcgcgtctt tcagcaaatg cgcgatgtag ctgctgaccg
    10321 tgatgatagt gacatttttc ttctgctccc ccagagtccg ggcgagcagg gcattgctgc
    10381 tgccattttc gataaagatg gtttcgcccg gttgaaccag tgacgcggca aactcggcga
    10441 gttcacgctt cagcgtatag ttgctcatca tacgggtttc gacgtcatca ctatcaagcg
    10501 aaactgcaaa gccatgtgca cggcggaggt aactcagttt ttcgagggtg ttgagatcct
    10561 ggcgaatggt aacttcagaa actccagtgg cttttgccag atcggttacg ctaacctgac
    10621 cctggtcaat gaccatctgt agaatagttt gttgtcggga attcatcgct gtaattctta
    10681 taacgttata atactaaata aaaaaatact gacgcgccgg gcatcctcaa ataggaaaac
    10741 ctgaaggcac gattgttaaa tctcccacgg tgccttcggg atgtctggcg tagattcagc
    10801 aacctggcgc aagagttctg ctttaagtat ctcgagatta tggatcgcag agtggtaatc
    10861 gcctgccaca aggatatcta acacaatatc aatacgttcg gcaacgcgct gggcatcaaa
    10921 ttcagacatc aaggacatcc ttatagcaaa agtggaaaat gaataatgca aaaattgccg
    10981 ctaaaagaga agtgtttaac agcaacggct aattatcatc caggaatacg atatataatg
    11041 acgggatata gcgctaagta tatatattca tctacttatg cgcgcttcag atagcgttta
    11101 taccagcgtt cgaaggcgac ggcgggcatc ggtttggcaa acaaaaatcc ttgccgctca
    11161 ttgatcccgt tcttggttaa aaaagcatct tcctttgcac tctctacacc ttcggcgatc
    11221 acctgaagat tcaatgcctg ggccacagcg acgatcgccc ggaccagtga ctgcgagaca
    11281 ggttgtttgt gaatatctcg aacaaaaacc tggtcaagtt tgatggcatc gatcggaaag
    11341 cgcgccagtt gcgaaagtga agagtagccg gtaccaaaat catccagatg cacttgcgca
    11401 cctagttggc taaattgttg aataacagac agtgccagtt catcattctc aatcagacaa
    11461 ctctctgtca gttcaacatc tatagggcag tattcaaaat tgagttcctg gagaacctgt
    11521 ttcagggcgg tgaaaatggt ttgatcggcg agctgacgtg cagaaatatt taccgccact
    11581 cgcaggttta tgcctttatc ccgccacttt gccacctggc gtacgacatc gagaatcacc
    11641 caacggccta aaggcacaat tagccctgac tcttcggcgt aggaaatgaa gtccaacggt
    11701 ggaatcaacc cacgttcagg tgactgccaa cgtactagtg cttccagact gcgcacttcg
    11761 ccacgccagg tgattttcgg ttgatagtga ataaccaact gatcgttttc cagtgcttta
    11821 cgcaagttgg tatccagcca gagatattca aatacccgtt gattcatttc tggggtaaaa
    11881 acgcaaaatt gtcctcgtcc gccttccttc gctgtgtaca ttgctgtgtc ggcgtgacga
    11941 ataatagccg tgctgtctga accatgttcg ggagagagtg caatacctac tgaacagctg
    12001 gtataaactt caattaaacc aatgcgaaag gggagccgta agcgggtcaa aattcgtgat
    12061 gccattgctt ccagcgcgct ttgtgaggtg ttggatgcca gtaccagaaa ctcatcccca
    12121 cctggacgcg ccaacacctg gtcatgttcg agacagctta aaatagccaa tgacacgtcg
    12181 cgtaataact ggtcaccaaa caaatgccca taggcgtcgt tgaccttttt gaaattatcc
    12241 aaatcaagat acacaacccc aactttattg ttatctgcat gattaatagc gtgatcgatt
    12301 aaatcctgca ttgcgttacg attcggcagt ccggtgatac tgtcggtatt tgccagaata
    12361 cgcagtcgct cctgagcgcg gcgctcttcg gtaatgtcgg tgccggaaca gattaaaaaa
    12421 atctcgcttt tgccactgcc gctgtggaca aatttattgc gaaacagaaa cagccgctgg
    12481 cctttacatg ttggtatcca cagttcgact tcatatgcat tgccgcttcg aaaaaataca
    12541 cggttattgc gcctggatgc cgcagcttca cgacggctca taaacagttt aaacacgctt
    12601 tggccaatga cgtcgtgttc tttcaaccca gtgtaatctt cacataaccg attgaagcgt
    12661 tggatattcc cccggctgtc gagaatcact attgcggaat gtgcttcaga aactacctgc
    12721 tcagcaaagg aaagcccgtg tgataaatca cgagcaactg ccggtgtgtc atgccatgca
    12781 gacgcgttgc cagcccattc ccgtttattg atttttcgtc ctaccagatg cacagaaaga
    12841 tcgctctcat cgacggtcag actcatcatc aggctggagg tgatgaccgt catttccctg
    12901 atgcgagcag cctgttcggc agataactgc aaagtacgat caggttctgt ggtttcggtg
    12961 gtagaaaagc gcaaaacatc gctgctttcc gtcaaccgcc agtatggatt gtgcgatccg
    13021 agaaagttgt acaacgttgt ggactcccta acggttttca tgatgcatat cctccggtta
    13081 aataaaagga acaacat
//
LOCUS       DAJVUJ010000118        12988 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.118, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000118 DAJVUJ010000000
VERSION     DAJVUJ010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12988
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.118"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(14..520)
                     /gene="mug"
                     /locus_tag="OQQ48_003012"
     CDS             complement(14..520)
                     /gene="mug"
                     /locus_tag="OQQ48_003012"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HCS1953702.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            645..720
                     /locus_tag="OQQ48_003013"
     tRNA            645..720
                     /locus_tag="OQQ48_003013"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:678..680,aa:Ile,seq:cat)
     gene            complement(774..1538)
                     /gene="nfeF"
                     /locus_tag="OQQ48_003014"
     CDS             complement(774..1538)
                     /gene="nfeF"
                     /locus_tag="OQQ48_003014"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
                     /protein_id="HCS1953703.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            1826..2449
                     /gene="nfeR"
                     /locus_tag="OQQ48_003015"
                     /gene_synonym="yqjI"
     CDS             1826..2449
                     /gene="nfeR"
                     /locus_tag="OQQ48_003015"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HCS1953704.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            complement(2603..4123)
                     /gene="aer"
                     /locus_tag="OQQ48_003016"
     CDS             complement(2603..4123)
                     /gene="aer"
                     /locus_tag="OQQ48_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HCS1953705.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSIIASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            4540..5919
                     /gene="ygjG"
                     /locus_tag="OQQ48_003017"
     CDS             4540..5919
                     /gene="ygjG"
                     /locus_tag="OQQ48_003017"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HCS1953706.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGSLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLLAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPQTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(5961..6293)
                     /locus_tag="OQQ48_003018"
     CDS             complement(5961..6293)
                     /locus_tag="OQQ48_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HCS1953707.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            6512..7495
                     /gene="ebgR"
                     /locus_tag="OQQ48_003019"
     CDS             6512..7495
                     /gene="ebgR"
                     /locus_tag="OQQ48_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HCS1953708.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLGIELTN
                     CYEHSGLPDIKNVTGILIVGKPTPALRAAACALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAQEDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            7679..10771
                     /gene="ebgA"
                     /locus_tag="OQQ48_003020"
     CDS             7679..10771
                     /gene="ebgA"
                     /locus_tag="OQQ48_003020"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HCS1953709.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHARDLTRGELKVENKLWFTT
                     LDDYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKM
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            10768..11217
                     /locus_tag="OQQ48_003021"
     CDS             10768..11217
                     /locus_tag="OQQ48_003021"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HCS1953710.1"
                     /translation="MRIIDNLEQFRQIYASSKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            11280..12713
                     /locus_tag="OQQ48_003022"
     CDS             11280..12713
                     /locus_tag="OQQ48_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCS1953711.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            12847..>12988
                     /locus_tag="OQQ48_003023"
     CDS             12847..>12988
                     /locus_tag="OQQ48_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1953712.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPS"
BASE COUNT         3133 a         3488 c         3248 g         3119 t
ORIGIN      
        1 tcggtggcgt tttttatcgc ccacgcacta ccagcgcctg gtccagctcg cgatacgctt
       61 caaccagttt ctctagtgaa acgcgactta aaccgctggg atttggcagc acccaaatct
      121 gcgtcgaacc aatggtgagc gtttgtttcc cccactgtgc accgcgctgg ctgaatccct
      181 gttcatatgc ttgtttgccc agaatcgcca acgcctgcgg ctgataatct tcaatttttt
      241 caatcagctt acgcccacct gcgtgcagct cctgctttga aacttcattg gcttgcaccg
      301 ttggacggtc taccagtttg gtgacgccac aacgataatc cagcagatgc tgtgcctcct
      361 gcggcttcaa ctgacggtcg gtaaacccgg cctgatatat caccttccag aagcgatttg
      421 ctggatgagc aaagggaaaa ccagtcccgg cggatgaaag cccagggttg ataccgcaaa
      481 acacgacccg taaccctgga gccaaaatat cctcaaccat atttactcct ttctaacatc
      541 accaggaaaa gtataacgga ttgaaaatac attgtttata aaaacagcag gcgcacggta
      601 atggctggat tgcgacacgg agttacttta taatccgcta ccatggcccc ttagctcagt
      661 ggttagagca ggcgactcat aatcgcttgg tcgctggttc aagtccagca ggggccacca
      721 gatatagcaa aggctgacga gaaatcgtca gcctttttct ttttatatct cagttacttt
      781 gcgtgccagt aagccgctgc acgtacccgc tgtgggtcat actgttccgc ttcaaagcgg
      841 cggcttaaat tcttaacgac tttaccttcg ccggttatcc agatgaagta atcatcggca
      901 gggatttgca tctgcgccag acgcgcatct accgcctgct catcatgtgc cagccattcg
      961 atattaaaac catcaaggtg cgcgagataa tcctgacagg cgttatcccg cacgctaacc
     1021 agcgcactaa cttgcggttt aacggcaagt ttgctcaacg tttccaggcg gcggcgcagt
     1081 gcgggcattc cggattcatc gcagacatac agctgatacg cgtaatcttc cggcaccacc
     1141 agcgaaccgc gcggaccagc caccgtaagt ttatcgcccg gttgcgcctg catcgcccag
     1201 ccgctggcga ccccaccgtc gtgaataaag aaatcgatcg ccagttcatg gcgtagttcg
     1261 tcatacagcg gcgtatagtc acgcgacggt gggcgtggtc cttccggcca gacgatgccc
     1321 tcttccgtta ccgttggcgg cacaaagtga gcgtcaggtt gaggaaagaa gagtttgctg
     1381 tgatcgtcaa agccgcgcga tgtaaaaccg tccagcgcct cgccgccgag gacaatgcgc
     1441 tgaaaaccgg cgctgatgcg ctcaacgcgt aacacagtca gttcacggaa gcgcagatca
     1501 ttgcgaacgc gctgcgggta gcggggggag ttattcattg ttatcgcctt cgtgatggta
     1561 atcagatata tctaaataat tttcgtaaat gataatgatt gttaatcatg ataaatgcaa
     1621 gcgattagta gaactgatat gtctatagtc tgataagacg aaccgcctct tctcaggcat
     1681 cattactcaa cgccggatgc ggcgtgaacg ccttatccgg cctacgtgtg agatgagtcc
     1741 acatttaaaa atcattttta ttcttgcaag agtgctcata tcagatataa attagatata
     1801 tctaatcaag aaaaaggagg ctgatatgag ccatcatcac gaagggtgtt gtaaacatga
     1861 aggccagcca cgccatgagg gctgctgcaa aggtgagaag tcagaacacg agcactgcgg
     1921 acacggtcac cagcatgaac acggtcaatg ctgcggtggt cgccacggtc gcggcggcgg
     1981 tcgtcggcaa cgtttctttg gtcacggtga attgcgtctg gtgattctgg atattctctc
     2041 gcgcgatgac agccacggtt acgaattgat taaagcgatt gagaatctaa cccaggggaa
     2101 ttacacccca agcccgggcg tcatctaccc aacgctggat tttctgcagg agcagtcgct
     2161 gattaccatc cgcgaagagg aaggtggtaa gaagcagatt gcgctgaccg aacaaggcgc
     2221 gcagtggctg gaagaaaacc gcgaacaggt ggagatgatt gaagaacgca tcaaagcgcg
     2281 ttgcgttggc gcggcgctgc gccagaaccc gcaaatgaag cgggcgctgg ataattttaa
     2341 agcggtgctg gatttacgcg tcaaccagag cgatatcagt gatgcacaaa taaaaaagat
     2401 cattgcggtg atcgaccgcg ccgcttttga tattacgcaa ctggattaat cgccgcatcc
     2461 gccagtggcg cggtgcaatt gccggatgcg acgcttgacg cgccttatcc ggcctacacc
     2521 cactacacac cccgcaggcc tgataagatg cgccagcatc gcatcaggca ttgtgctcca
     2581 accgccggat ccggcatacc gattaatgca gcaccgtcac cgcgtcttcc agtcggctgg
     2641 cgcggtgttt caccatcgcc gacacctgcg cactctcttc caccagctca gcatttttct
     2701 gggtgatcag gttaagctca tccactgcac gggtcaggct ggaaagccca tcggcctgtt
     2761 ccagcgttga atggctaatc tgggcgatca actgggtgac gtttttcacc tgtgccacaa
     2821 tatcttccat cgtccgtccg gcggcgtgta cctgctgcga accggattgc accttatcag
     2881 cactggcatc aatcagcttg cgaatatcgt tggcagcatt agcgctgcgg ctggctaaat
     2941 gacgcacttc ccctgccacc actgcaaaac ctttgccctg ttcgccggca cgcgccgctt
     3001 ccaccgccgc attcagggcc agaatattgg tctgaaacgc aatatcgtta atcagcgaag
     3061 taatggtgcc aatgcgctgg gtactgtcgg cgatatcgtc cattgtcttg atcaccgtgg
     3121 tcatcgcctc cccaccctgc actgccgcat tactggcgat gatagaaagt ttatcggcag
     3181 ccgacgccgt ggcagagttc tgtttcaccg acgccgccat ttggttcatg gtcgccaccg
     3241 tttgctgaac gttatcaact gtctgctggg tatgttcgtt cagttcatcg gtgcctttcg
     3301 ccagcgtctc actgccattt ctgacactgg acacctggct tgagacatcg ttaattagcc
     3361 aacggcacat caggccaagc tgccctaccg cgcgtaatgt cagccccagc tcatcgctgc
     3421 gattcaggtg ctcaacacta ttacgctctc cggtcgccac cttcagtgcc tgacgggcga
     3481 cattttctat cgggcgcaca atctgccatt caaaacaagc gcttgccaac aataccacta
     3541 acacacagag gaaatacgtc accaccgggg cagcaacaaa ccaaagcatg gccgccagca
     3601 agataaacat cagggtcatc actccacgcg cccgccagcg aagcggtaat gaaggcagtt
     3661 tacccagcca gcctttacgc accaccaggc ctttatgaat acgcttactg gtacgtccgg
     3721 cgttcagcgc tttgtacagc ggctccaccg ccgcaatctc ttcatccgtc gcccgggtac
     3781 gaatcgacat atagccactg atttttccct cgcgcaccat cggtaccgca ttggcccgca
     3841 cccaataatg gtcgccattt ttgcggcgat ttttcacgat gccgctccag ggctcccctt
     3901 ttttcagggt gaaccacata tccgcaaacg ccgcttttgg catatccggg tgacgcacca
     3961 tgttgtgcgg ctgcccttgt aactcttgca aggtaaagcc gctcacctgc acaaaagtgt
     4021 cattagcatg agtgatatag ctttgcagat cggtagtgga catcagagtg gtatcgtccg
     4081 ccagcggggt attttgctgg gtgacatacg gatgagaaga catgttcgcg tcctatgcag
     4141 gttatatggt tgttaacttc ttgtcagagt ttatgtcggc cccgctgcgg ttatctttaa
     4201 ccgattaatt tgatttagat cgcaatttgc gattaaagca caaatctaat tccttgattt
     4261 aaaatacttt cactctgtta ctatacgaaa acgttaatta tcttgcccaa aatcaggcaa
     4321 ttatcgccct gaaaacgtgc atttgcacag caatcatcaa atccataccc gacaaaaacc
     4381 gtgcaaaata acaacgaatg ttaacaaata gcattaaata ctgcgcaaat gataaccaca
     4441 tttgtgttta tcccgatttt cgcgatcgca gccggagtgg cgcaatccct gcaatactta
     4501 aatcggtatc atgtgatacg cgagcctccg gagcatattt tgaacaggtt accttcgagc
     4561 gcatcggctt tagcgtgcag cgcccacgcc ctgaatctca ttgagaagcg aacgctggat
     4621 catgaggaga tgaaagcact taaccgagag gtgattgaat acttcaaaga gcatgtcaat
     4681 ccggggtttt tagagtatcg caaatctgtt accgccggcg gggattacgg agccgtagag
     4741 tggcaagcgg gaagtttaaa tacgcttgtc gacacccagg gacaggagtt tatcgactgc
     4801 ctgggaggtt ttggaatttt caacgtgggg caccgtaatc cagttgtggt ttccgccgta
     4861 cagaatcaac ttgcgaaaca accgctgcac agccaggagc tgctcgatcc gttacgggcg
     4921 atgttggcga aaacccttgc tgcgctaacg cccggtaaac tgaaatacag cttcttctgt
     4981 aatagcggca ccgagtccgt tgaagcagcg ctgaagctgg cgaaagctta ccagtcaccg
     5041 cgcggcaagt ttacttttat tgccaccagc ggcgcgttcc acggtaaatc acttggcgcg
     5101 ctgtcggcca cggcgaaatc gaccttccgc aaaccgttta tgccgttact gccgggcttc
     5161 cgtcatgtgc cgtttggcaa tatcgaagcc atgcgcacgg ctcttaacga gtgcaaaaaa
     5221 accggtgatg atgtggctgc ggtgatcctc gaaccgattc agggtgaagg tggcgtaatt
     5281 ctgccgccgc cgggctatct caccgccgta cgtaagctat gcgatgagtt cggcgcactg
     5341 atgatcctcg atgaagtaca aacgggcatg gggcgcacgg gcaagatgtt cgcctgcgag
     5401 catgagaacg tacagccgga tatcctctgc cttgccaaag cgctcggcgg cggcgtgatg
     5461 ccgattggcg cgaccatcgc cactgaagag gtgttttcag ttctgttcga caacccattc
     5521 ctgcatacca ccacctttgg cggcaacccg ctggcctgtg cggcggcgct ggcgaccatc
     5581 aatgtgttgc tggagcagaa cttactggct caggctgagc aaaaaggcga tatgttgctg
     5641 gacggtttcc gtcaactggc gcgggaatat cccgatctgg tacaggaagc gcgtggtaaa
     5701 gggatgttga tggcaattga gtttgttgat aacgaaatcg gctataactt tgccagcgag
     5761 atgttccgcc agcgcgtact ggtggccgga acgctcaata acgccaaaac gatccgcatt
     5821 gaaccgccac agacactgac cattgaacag tgtgaactgg tgatcaaagc ggcgcgtaag
     5881 gcgctggcgg ccatgcgagt aagtgtcgaa gaagcgtaat atcaaatcgg atggcgatac
     5941 gacgtcgcca tccgatcttt ttatacgacg cgtacgcccg caggcatcat ccgctccggc
     6001 gttaacaaca cgctttcact gccgtcatcg gtttccgcac acaacagcat gcattccgac
     6061 gtttcaccac gcattttcgc tttttgcaga ttgcataaga ccaccaccgt tttccccatc
     6121 agctcttctt cgctgtagta cggcaccagg ctggtcacgg tttgcagtgt tttttccccc
     6181 acatcaacct gtacgatgta cagcttgtcg gcgttttcat ggcgtttcac ttccacaatc
     6241 tttccgacgc gcatttccag acgtgcaaaa tcagcgtaag ccacggtttc cattgtttcc
     6301 ttccttcggg taaaatttta ctaaactata gcaaaagttt ttctcaatcc tgtaggctaa
     6361 aaatggagaa tgcaggcgtg atcacattcg tgagccgctg tgttaccgtt acagcgtcaa
     6421 agaaacgcgc tttatttact gaaaacaggt gacccgataa gtacttcctc tacaatgggg
     6481 gcgcacatca gggaaagtaa aaaaggtaaa catggcaaca ctaaaagaca tcgcaatcga
     6541 agctggcgta tccctggcga cagtatccag ggtcttaaat gacgatccga cattgaatgt
     6601 gaaagaagag acaaaacatc gcattctcga gatcgccgaa aagctggagt acaagaccag
     6661 tagtgcccgc aaactccaga caggtgcagt caaccaacac catattctgg ctatctacag
     6721 ctaccaacag gagctggaga tcaacgatcc ttactatctg gcgatccgcc acggcattga
     6781 aactcagtgc gaaaagctgg gcattgagct caccaactgt tatgaacaca gcggattacc
     6841 agacattaaa aacgtcaccg gtattttaat tgtcggcaaa cccacgcccg cccttcgcgc
     6901 cgccgcctgt gcgttgaccg acaatatctg ttttatcgac tttcacgaac ccggcagcgg
     6961 ttacgatgcg gtggatatcg atctggcacg catcagtaaa gaaatcatcg acttctatat
     7021 caatcagggc gttaatcgta ttggttttat tggcggtgaa gatgagccag gcaaagcgga
     7081 tattcgtgag gtcgcctttg cggaatatgg ccgactgaaa caagtggtac gcgaagagga
     7141 tatctggcgc ggaggctttt ccagttcgtc gggttatgaa ctggcaaaac agatgctggc
     7201 gcaggaagac tatccgaagg cactgtttgt tgcttccgat tccattgcta tcggcgtact
     7261 gcgggcaatt catgaacgag gcctgaacat cccacaggat atttcgctta tcagcgttaa
     7321 cgatatcccc accgcgcgat ttacctttcc gccgctctcc accgtgcgca tccattccga
     7381 gatgatggga agtcagggcg ttaacctggt gtatgaaaaa gcccgcgatg gtcgcgcgct
     7441 gccgctgtta gtcttcgttc ccagcaaatt aaaactgcgc ggcacgaccc gttaaatccc
     7501 cttacacact gtccggcaat cgtttttgcc ggacagtgct gccgtttatt ttcgtgatcc
     7561 agttaaagta aatgcattta tctgctactt tttagtaaaa attttactaa actccccagc
     7621 aattacacaa actaccatca ccatgaatgg ttccgatttc tctctaccgg gaggccctat
     7681 gaatcgctgg gaaaacattc agctcaccca cgaaaaccga cttgcgccgc gtgcgtactt
     7741 tttttcatat gattctgttg cgcaagcgcg tacctttgcc cgcgaaacca gcagcctgtt
     7801 tctgccctta agcggtcagt ggaatttcca cttttttgac catccgctgc aagtaccaga
     7861 agccttcacc tctgagttaa tggctgactg ggggcatatt accgtccccg ccatgtggca
     7921 aatggaaggt cacggcaaac tgcaatatac cgacgaaggt tttccgttcc ccatcgatgt
     7981 gccgtttgtc cccagcgata acccaaccgg tgcctatcaa cgtattttca ccctcagcga
     8041 cggctggcag ggtaaacaga cgctgattaa atttgacggc gtcgaaacct attttgaagt
     8101 ctatgttaac ggtcagtatg tgggtttcag caagggcagt cgcctgaccg cagagtttga
     8161 catcagcgcg atggttaaaa ccggcgacaa cctgttgtgt gtgcgcgtga tgcagtgggc
     8221 ggactctacc tacgtggaag accaggatat gtggtggtca gcggggatct tccgcgatgt
     8281 ttatctggtc ggaaaacacc taacgcatat taacgatttc actgtgcgta ccgactttga
     8341 cgaagcctat tgcgatgcca cgctttcctg cgaagtggtg ctggaaaatc tcgccgcctc
     8401 ccctgtcgtc acgacgctgg aatataccct gtttgatggc gaacgcgtgg tgcacagcag
     8461 cgccattgat catttggcaa ttgaaaaact gaccagcgcc agctttgctt ttactgtcga
     8521 acagccgcag caatggtcag cagaatcccc ttatctttac catctggtca tgacgctgaa
     8581 agacgccaac ggcaacgttc tggaagtggt gccacaacgc gttggcttcc gtgatatcaa
     8641 agtgcgcgac ggtctgttct ggatcaataa ccgttatgtg atgctgcacg gcgtcaaccg
     8701 tcacgacaac gatcatcgca aaggccgcgc cgttggaatg gatcgcgtcg agaaagatct
     8761 ccagttgatg aagcagcaca atatcaactc cgtgcgtacc gctcactacc cgaacgatcc
     8821 gcgtttttac gaactgtgtg atatctacgg cctgtttgtg atggcggaaa ccgacgtcga
     8881 atcgcacggc tttgctaatg tcggcgatat cagccgtatt accgacgatc cgcagtggga
     8941 aaaagtctac gtcgagcgca ttgttcgcca tatccacgcg cagaaaaacc atccgtcgat
     9001 catcatctgg tcgctgggca atgaatccgg ctatggctgt aacatccgcg cgatgtacca
     9061 tgcggcgaaa gcgctggatg acacgcgact ggtgcattac gaagaagatc gcgatgctga
     9121 agtggtcgat attatttcca ccatgtacac ccgcgtgccg ctgatgaatg agtttggtga
     9181 atacccgcat ccgaagccgc gcatcatctg tgaatatgct catgcgatgg ggaacggacc
     9241 gggcgggctg acggagtacc agaacgtctt ctataagcac gattgcattc agggtcatta
     9301 tgtctgggag tggtgcgacc acgggatcca ggcacaggac gaccacggca atgtctggta
     9361 taaattcggc ggcgactacg gcgactatcc caacaactat aacttctgtc ttgatggttt
     9421 gatctattcc gatcagacgc cgggaccggg cctgaaagag tacaaacagg ttatcgcgcc
     9481 ggtaaaaatc cacgcgcggg atctgactcg cggcgagttg aaagtcgaaa ataaactgtg
     9541 gtttaccacg cttgatgact acaccctgca cgcagaggtg cgcgccgaag gtgaaacgct
     9601 cgcgacgcag cagattaaac tgcgcgacgt tgcgccgaac agcgaagccc ccttgcagat
     9661 cacgctgccg cagctggacg cccgcgaagc gttcctcaac attacggtga ccaaagattc
     9721 ccgcacccgc tacagcgaag ccgggcattc tatcgccact tatcagttcc cgctgaagga
     9781 aaacaccgcg cagccagtgc ctttcgcacc aaataatgcg cgtccgctga cgctggaaga
     9841 cgatcgtttg agctgcaccg ttcgcggcta caacttcgcg atcaccttct caaaaatgag
     9901 tggcaaaccg acatcctggc aggtaaatgg cgagtcgctg ctgacccgcg agccaaagat
     9961 caacttcttc aagccgatga tcgacaacca caagcaggag tacgaagggt tgtggcagcc
    10021 gaatcatttg cagatcatgc aggaacatct gcgcgacttt gccgtagaac agagcgatgg
    10081 tgaagtgttg atcatcagcc gcacagttat tgcaccgccg gtgtttgact tcgggatgcg
    10141 ctgtacctac atctggcgca tcgctgccga tggccaggtt aacgtggcgc tttccggcga
    10201 gcgttacggc gactatccgc acatcattcc gtgcatcggt ttcaccatgg ggattaacgg
    10261 cgaatacgat caggtggcgt attacggtcg tggaccgggc gaaaactacg ccgacagcca
    10321 gcaggctaac atcatcgata tctggcgcag caccgtcgat gccatgttcg agaactatcc
    10381 cttcccgcag aacaacggca accgtcagca tgtccgctgg acggcactga ctaaccgcca
    10441 cggcaacggt ctgctggtgg ttccgcagcg cccaattaac ttcagcgcct ggcactatac
    10501 ccaggaaaac atccacgctg cccagcactg taacgagctg cagcgcagtg atgacatcac
    10561 cctgaacctc gaccaccagc tacttggcct cggctccaac tcctggggca gcgaggtgct
    10621 ggactcctgg cgcgtctggt tccgtgactt cagctacggc tttacgttgc tgccggtttc
    10681 tggcggagaa gctaccgcgc aaagtctggc gtcgtatgag ttcggcgcag ggttcttttc
    10741 cacgaatttg cacagcgaga ataagcaatg aggatcatcg ataacttaga acagttccgc
    10801 cagatttacg cctctagcaa gaagtggcaa cgctgcgttg aagcgattga aaatatcgac
    10861 aacattcagc ctggcgtcgc ccactccatc ggtgactcat tgacttaccg cgtggagaca
    10921 gactccgcga ccgatgcgct atttaccggg catcgacgct attttgaagt gcattactac
    10981 ctgcaagggc agcaaaaaat tgaatatgcg ccgaaagaga cattacaggt agtggaatat
    11041 tatcgtgatg aaactgaccg tgaatattta aaaggctgcg gagaaaccgt tgaggtccac
    11101 gaagggcaaa tcgttatttg cgatatccat gaagcgtatc ggtttatctg caataacgcg
    11161 gtcaaaaaag tggttctcaa agtcaccatc gaagatggtt atttccataa caaataacaa
    11221 ctacggcggc aaaaggagtt tgccgccacc gctaccctac acattttcgg agatgtgtta
    11281 tgtctgatac caaacgtaat acaatcggca aattcggctt gctctcgctg acttttgctg
    11341 ccgtttacag ctttaacaac gttatcaata ataatattga gcttggactg gcctcggcac
    11401 cgatgttttt cctcgcgacg attttttatt ttattccctt ctgtctgatc atcgcagaat
    11461 ttgtttcgtt aaataaaaac tcagaagccg gtgtctacgc gtgggtaaaa agttcgctgg
    11521 gcggacgttg ggcatttatt actgcctata cctactggtt cgtaaacctg ttctttttca
    11581 cctcactgtt gccgcgcgtt attgcttatg cttcgtatgc cttcctcgga tacgaatata
    11641 ttatgacgcc ggttgccacc accattatca gtatggtgct gttcgccttc tccacctggg
    11701 tttccaccaa cggggcgaaa atgttggggc caattacctc cgtcacttca acgctgatgc
    11761 tgctgttaac gctctcctac attttactgg caggtacggc gctggttggc ggcgtacagc
    11821 ctgctgaccc catcaccgtt gacgcgatga tcccgaactt caactgggcg ttcctcggcg
    11881 ttaccacctg gatctttatg gccgcaggtg gcgcggagtc cgtcgctgtg tacgttaacg
    11941 acgtcaaagg cggttcgaaa tcgttcgtta aagtgatcat cctcgccggg atttttatcg
    12001 gcgtactgta ttccgtctcc tcggtgctga ttaacgtctt cgtcagcagc aaagagttga
    12061 aatttaccgg cggatcggtg caggtattcc atggcatggc ggcgtatttt ggtctaccgg
    12121 aagcgttgat gaatcgcttt gtcggtctgg tgtcctttac cgcgatgttc ggttccctgc
    12181 tgatgtggac cgcaacgccg gtgaaaattt tcttctccga aatcccggaa ggcatctttg
    12241 gtaagaaaac cgtcgaactg aacgaaaacg gcgttccggc gcgcgcagcg tggatccagt
    12301 tcctgatcgt catcccgctg atgattatcc cgatgctcgg ttccaatacc gtgcaggatc
    12361 tgatgaatac tattattaat atgaccgccg cagcgtccat gcttccgccg ttattcatca
    12421 tgctggctta cctgaattta cgcgccaaat tagatcacct gccacgcgat ttccgtatgg
    12481 gctcccgccg caccggtatt atcgttgttt caatgctgat tgcgatattt gccgtagggt
    12541 ttgtcgcttc gacattcccg actggcgcga atattctgac catcattttt tataacgtcg
    12601 gcggtattgt tatcttcctt ggctttgcgt ggtggaaata cagtaaatat ataaagggat
    12661 taacggctga agagcgccat attgaagcga cgccagccag caatgttgat taagcagaat
    12721 aataaaaaat gagcaggcat tttccctctt cctggatgag gagagggaaa atagtttctg
    12781 cctttatatt tttataatta caacgataaa aggctgtact ttttctttag ctcatggatt
    12841 aacacaatga aattaatcac tgcaccatgc agagcattac ttgctctgcc gttttgctac
    12901 gccttttctg cggcaggaga agaagcacgt ccggcagaac atgacgacac aaaaacaccc
    12961 gcaattacct cgacatcttc tccttcat
//
LOCUS       DAJVUJ010000119        12942 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.119, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000119 DAJVUJ010000000
VERSION     DAJVUJ010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12942
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.119"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(234..1574)
                     /gene="fadL"
                     /locus_tag="OQQ48_003024"
     CDS             complement(234..1574)
                     /gene="fadL"
                     /locus_tag="OQQ48_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HCS1953713.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGNTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            1946..2230
                     /locus_tag="OQQ48_003025"
     CDS             1946..2230
                     /locus_tag="OQQ48_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HCS1953714.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            2410..3720
                     /gene="fadI"
                     /locus_tag="OQQ48_003026"
     CDS             2410..3720
                     /gene="fadI"
                     /locus_tag="OQQ48_003026"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HCS1953715.1"
                     /translation="MGQVLPLVTRQGDRIAIVSCLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLFGPAWSTPLALERAGLTM
                     SDLTLIDMHEAFAAQTLANIQLLGSERFACEVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGAWMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            3720..5864
                     /gene="fadJ"
                     /locus_tag="OQQ48_003027"
     CDS             3720..5864
                     /gene="fadJ"
                     /locus_tag="OQQ48_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HCS1953716.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTVQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTTLEMILTGKQLRAKQALKLGLVDDVVPHSILLEVAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTKGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVSERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            6067..6552
                     /gene="sixA"
                     /locus_tag="OQQ48_003028"
     CDS             6067..6552
                     /gene="sixA"
                     /locus_tag="OQQ48_003028"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HCS1953717.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            7227..7487
                     /locus_tag="OQQ48_003029"
     CDS             7227..7487
                     /locus_tag="OQQ48_003029"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCS1953718.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDT"
     gene            7494..7790
                     /locus_tag="OQQ48_003030"
     CDS             7494..7790
                     /locus_tag="OQQ48_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1953719.1"
                     /translation="MTTTFTGTVSSANSGNYYTIFNTDTGAAFNNVSLAIGDSLGTSY
                     KSGMGIDQKIVKDTSTNKGKAKQTLNFKAWLVGAADAPDLGNFEANTTFQITYL"
     gene            7872..10514
                     /gene="yfcU"
                     /locus_tag="OQQ48_003031"
     CDS             7872..10514
                     /gene="yfcU"
                     /locus_tag="OQQ48_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
                     /protein_id="HCS1953720.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDWD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEFSGDETQKKWEWSRYYAWRALPSLKAKLALGEDYLRSDIFDGFNYVGG
                     SVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDSV
                     SGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQIRYKIMMGRPQEWGHHVEGEFFSGA
                     EASWGIANGWSLYGGALGDENYQSAALGVGRDLSTFGAVAFDVTHSHTKLDKDTAYGK
                     GSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENFMTMSEYLDASDSGMVRTGNDKEM
                     CTATYNQNFRDAGVSVYLNYTRHTYWDREEQINYNIMLSHYFNMGSIRNVSISMTGYR
                     YEYDNQADKGMYISLSMPWGDNSTVSYNGNYGSGTDSSQVGYFSRVDDATHYQLNVGT
                     SDKHTSVDGYYSHDGSLAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMG
                     GTRLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNRLPENAEAT
                     QSVVQATLTEGAIGYRKFAVISGQKAMAVLRLSDGSHPPFGAEVKNDNEQTVGLVDDD
                     GNVYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPITSPAV
                     KPAIQEQTQRVTPTEPPTSISVNQ"
     gene            10534..11286
                     /locus_tag="OQQ48_003032"
     CDS             10534..11286
                     /locus_tag="OQQ48_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000482750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCS1953721.1"
                     /translation="MFNLTNTAKIVVPALALLATAVSFSSHASVTPDRTRLVFNESDK
                     SISVTLRNNDPTLPYLAQSWIEDAKGNKISSPLAVLPPVQRIDAMMNGQVKVQGLPDI
                     NKLPADRESVFYFNVREISPKSNKPNTLQIALQTRIKLFWRPKALENTSMKSPWQHKV
                     TLIRNGQDFTVNNPTPYYVIISNASAKKGGNPPAGFTPLMMEPKTSAPLNAKMDSVPV
                     LTYINDYGARMPLYFKCTGNSCQVDEEQSRKG"
     gene            11302..11811
                     /locus_tag="OQQ48_003033"
     CDS             11302..11811
                     /locus_tag="OQQ48_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001733155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1953722.1"
                     /translation="MSHKFVAGAGLLVLAGWGISGLAVAGSDIKQVDLTLRILVDGPP
                     PCSVKGSAVKFGNVIINRIDGTNYQQDAKYTLNCGNKVSDDLRMQLKGNTAVINGETV
                     LSTDIAGLGVRIENAADNSLFAVGENNWTPFNVNTQPQLKAVPVKQSGVQLTPSEFNA
                     TMTMVVDYQ"
     gene            11808..12296
                     /locus_tag="OQQ48_003034"
     CDS             11808..12296
                     /locus_tag="OQQ48_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1953723.1"
                     /translation="MKKFLLLLLCAGCGAVLAAEDDITFHGTLMSPPACSISGGQTIE
                     VQFRDLIIDSIDGNYGRQKVDYELSCDSDVRDPEWNMTLSWTGTATSYNDSAIETDVP
                     GFGIELQHDGQRFKLNEPLSINATDFSQKPKLEAVPVKAADAVLTDTTFSAYATLRVD
                     YQ"
     gene            12293..12832
                     /locus_tag="OQQ48_003035"
                     /pseudo
     CDS             12293..12832
                     /locus_tag="OQQ48_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416836.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
BASE COUNT         3258 a         3082 c         3437 g         3165 t
ORIGIN      
        1 gtatcctgga aatgcagaaa agtaaactct ttgagaagga cgagcagaac gcctgaaaat
       61 atgcctttcc cttcaggttt gtctgtggat accgcttatt gatttgaaag ggcaagcggc
      121 caacaggctg tttgcaaaac tcaggaggta agcaatgagg acactacttt cggtgaagtg
      181 ggggagttaa agtcacctgc tatgcaggtg actttatcca ggcgaacgcg ttatcagaac
      241 gcgtagttaa agttagtacc gaacagccag gctttacctt cagactcgaa ctggtatggg
      301 ccttcgttaa ttttcacgct ctgaccgtgc atataagaaa caccaacgtc gactgaagca
      361 tctttattaa atgcgtaagt cgtacctgca ctcagccaga aacggtcctg gtccggaatg
      421 gagatagaac gattctgagc cggaactggg ctgtcatcaa aggcgatacc ggtacggaag
      481 gtccagttat catcgtagta ataagtggta cccaacgcga tgcggtaagc atctttaaag
      541 ccttcatgtt tctggaacag cgtgttgcca ctggttgagg tcgctttcag ctgctggaac
      601 tgactccagc tggtgtaagc caggctatag tgaatcgccc actgtggatc aacacggtta
      661 taacctgaca cttcccacat ttcaggcagg ttcagcgtca gataacccga ttgcgttgcg
      721 ccacctgtcg cggtaggaat tggcaaaccg tagttattaa acgcacgatt aagatcgctg
      781 ctgtagttac ctttgaagtc aattttcact tcagaacggt aggtcagtgc atagcggtta
      841 tttttatcca gttcatacag gattccggcg ttccagccaa agccccactg gttaccgttc
      901 agatgagcga ttttggtatt actgtcgata ccgttggcgg tagctgccaa tgcttgccct
      961 tgaggagttt gcccggcagg agattgcata atctgaccag caaccaactg ccccagatcg
     1021 cctgcgaaac gttcaatttt cgcgcgagcg tagacggcgt tgaaaccaag accaaagctc
     1081 catgcattat ttaagcgata cgcaccgctt aagttcaggt tcatggtttc aaggtcagtt
     1141 gtacccccga cagagccgcc tgcataagta tcattaaact ctgttgccag accatagtta
     1201 gaggtaatag aagcgcccca accaaattgg tcgttaatcg gtgcaacaaa gtgcatgttc
     1261 ggaacccatg ccgtaggcgc gatgttatcg gctttcaggc tacgaccaga cggagacgtt
     1321 ccgctgatat tcacatccgg gtcaatataa accgcacccg cagaaaatgt cgggcggtca
     1381 aacatggtaa tcaatgcggg gttacggcta acgttacctg catcatcggc aattgcgcct
     1441 tcccctgaat aagcccggcc caggccagag gaagaaaatt cgtttaactg aaagcctgcc
     1501 gaccaggcct gggtggagat aagtgccact gcgactgcga gagcagactt tgtaaacagg
     1561 gttttctggc tcatgaccat aacctcaatg atttattttt atacaaacta tgttacgtgc
     1621 tgtaacagga gcgcgaagtg tagggtctga ggtacatctt agaaatcaga ccagtggcga
     1681 gagtataggt cggaccagct ggaatgttgc aagtatgttt ccatcttttt tcaaatatga
     1741 tctaagaaac gcgatccagc tggcaaaatt tggaggttgc gcactcgcgg attcgggtga
     1801 attttgtttt agatcatttt taagtgtgat ttcggtcact tatccgattt tgcagaatta
     1861 acaactggag cagcactttc cggaagcgta aaatatccac aacgtttatc tggcaccttg
     1921 ggtgcacata cagaggaaat caacgatgag taaatgtagt gctgatgaaa ccccggtttg
     1981 ctgctgtatg gatgttggca ccattatgga caactccgac tgcaccgcgt cttacagccg
     2041 cgtgtttgca aaccgcgcag aagcagaaca aacgctggcg gcgttgactg aaaaagcccg
     2101 tagcgtggaa tccgaaccgt gcaaaatctc cccgactttc actgaagagt ctgacggtgt
     2161 gcgcctggat atcgatttca cttttgcctg cgaagctgaa atgctgatct tccagctggg
     2221 tctgcgttaa ttcctctcgg gtacgttcgc ccctgtcagt tgctggcagg ggcgttttct
     2281 tttctttctc ctgtcttcac tgtgtcatag ctcccacttt cctttttctc cgcttggcta
     2341 aatgtaaaaa aatggttaag actataatca ggtcagacca ctttatttat ttttttacag
     2401 gggagtgtta tgggtcaggt tttaccgctg gttacccgcc agggcgatcg tatcgccatt
     2461 gttagctgtt tacgtacgcc ttttgcccgc caggcgacgg cttttcatgg cattcccgcg
     2521 gttgatttag ggaagatggt ggtaggcgaa ctgctggcac gcagcgagat ccccgctgaa
     2581 gtgattgaac agctggtctt tggtcaggtc gtacaaatgc ctgaagcccc caacattgcg
     2641 cgtgaaattg ttctcggtac cggaatgaat gtgcataccg atgcttacag cgtcagccgc
     2701 gcttgcgcta ccagtttcca ggcggttgca aacgtcgcag aaagcctgat ggcgggaact
     2761 atccgagcgg ggattgccgg tggggcagat tcctcttccg tattgccaat tggcgtcagt
     2821 aaaaaactgg cgcgcgtgct ggttgatgtc aacaaagctc gaaccatgag ccagcgcctg
     2881 aaactcttct ctcgcctgcg tttgcgcgat ttaatgcccg taccgcctgc agtagcagaa
     2941 tattctaccg gcttgcggat gggtgacacc gcagagcaaa tggcgaaaac ctacggcatc
     3001 acccgagaac agcaagatgc attagcgcac cgttcgcatc agcgtgccgc tcaggcatgg
     3061 tcagacggaa aactcaaaga agaggtgatg actgccttta tccctcctta taaacaaccg
     3121 cttgtcgaag acaacaatat tcgcggtaat tcctcgcttg ccgattacgc aaagctgcgc
     3181 ccggcgtttg atcgcaaaca cggaacggta acggcggcaa acagtacgcc gctgaccgat
     3241 ggcgcggcag cggtgatcct gatgactgaa tcccgggcga aagaattagg gctggtgccg
     3301 ctggggtatc tgcgcagcta cgcatttact gcgattgatg tctggcagga catgttgttc
     3361 ggtccggcct ggtcaacacc gctggcgctg gaacgtgccg gtttgacaat gagcgatctg
     3421 acattgatcg atatgcacga agcctttgca gctcagacac tggcgaatat tcagttgctg
     3481 ggtagtgaac gttttgcttg tgaagtactg gggcgtgcac atgccactgg cgaagtggac
     3541 gatagcaaat ttaacgtgct tggcggttcg attgcttacg ggcatccctt cgcggcgacc
     3601 ggcgcgtgga tgattaccca gacattgcat gaacttcgcc gtcgcggcgg tggatttggt
     3661 ttagttaccg cctgtgctgc cggtgggctt ggcgcggcaa tggttctgga ggcggaataa
     3721 tggaaatggc atcagcgttt actcttaatg ttcgtctgga caacattgct atcatcacca
     3781 tcgacgtacc ggatgagaaa atgaataccc tgaaggcgga atttgcctcg caggtgcgcg
     3841 ccattattaa gcaactccgt gaaaacaaag agttgcgcgg cgtggtgttt atctccgcga
     3901 aaccggacaa ttttatcgcc ggagcagaca tcaacatgat cggcaactgc aaaacggtgc
     3961 aagaagcgga agctctggcg cggcagggcc aacagttgat ggcggagatt catgctttgc
     4021 ccattccggt tatcgcggct attcatggcg cttgcctggg tggtgggctg gagttggcgc
     4081 tggcgtgcca cggtcgcgtt tgtactgacg atcctaaaac ggtgctcggt ttgcctgaag
     4141 tacaacttgg attgttaccc ggttcaggcg gcacccagcg tttaccgcgt ctgataggcg
     4201 tcagcacaac attagagatg atcctcaccg gaaaacaact tcgggcgaaa caggcattaa
     4261 agctggggct ggtggatgac gttgttccgc actccatttt gctggaagtc gctgttgagc
     4321 tggcgaagaa ggatcgccca tcttcccgcc ctctacctgt acgcgagcgt attctggcgg
     4381 ggccgttagg tcgtgcgctg ctgttcaaaa tggtcggcaa gaaaacagaa cacaaaactc
     4441 aaggcaatta tccggcgaca gaacgcatcc tggaggttgt tgaaacggga ttagcgcagg
     4501 gcaccagcag cggttatgac gccgaagctc gggcgtttgg cgaactggcg atgacgccac
     4561 aatcgcaggc gctgcgtagt atcttttttg ccagtacgga cgtgaagaaa gatcccggta
     4621 gtgatgcacc gcctgcgcca ttaaacagcg tgggaatttt aggtggtggc ttgatgggcg
     4681 gcggtattgc ttatgtcact gcttgtaaag cggggcttcc ggtcagaatt aaagatatca
     4741 acccgcaggg cataaatcat gcgctgaagt acagttggga tcagctggag ggcaaagttc
     4801 gccgtcgtca tctcaaagcc agcgaacgtg acaaacagct ggcattaatc tccggaacga
     4861 cggactatcg cggctttgcc catcgcgatc tgattattga agcggtgttt gaaaatctcg
     4921 aattgaaaca acagatggtg gcggaagttg agcaaaattg cgccgctcat accatctttg
     4981 cttcgaatac gtcatcttta ccgattggtg atatcgccgc tcacgccacg cgacctgagc
     5041 aagttatcgg cctgcatttc ttcagtccgg tggaaaaaat gccgctggtg gagattattc
     5101 ctcatgcggg tacatcagcg caaaccatcg ccactacggt aaaactggcg aaaaaacagg
     5161 gcaaaacgcc cattgtcgtg cgtgacaaag ccgggtttta cgtcaatcgc atcttagcgc
     5221 cttacattaa tgaagctatc cgcatgctga ccaaaggtga acgggtagag cacattgatg
     5281 ccgcgctagt gaaatttggt tttccggtag gtccaatcca acttttggat gaggtaggaa
     5341 tcgacaccgg gactaaaatt attcctgtac tggaagccgc ttatggagaa cgttttagcg
     5401 cgcctgcaaa tgttgtttct tcaattttga acgacgatcg caaaggcaga aaaaatggtc
     5461 ggggtttcta tctttatggt cagaaagggc gtaaaagcaa aaaacaggtc gatcccgcca
     5521 tttacccgct cattggcgca caagggcagg ggcgactctc cgcaccgcag gtttctgaac
     5581 ggtgtgtgat gttgatgctg aatgaagcag tacgttgtgt tgatgagcag gttatccgta
     5641 gcgtgcgtga cggggatatt ggtgcggtat ttggcattgg ttttccgcca tttctcggtg
     5701 gaccgttccg ctatatcgat tctctcggcg cgggcgaagt ggttgcaata atgcaacgac
     5761 tggccacgca gtatggttcc cgttttaccc cttgcgagcg tttggtcgag atgggcgcgc
     5821 gtggggaaag tttttggaaa acaactgcaa ctgacctgca ataagaaggt caaagctata
     5881 tgaatccgcg ctgaatggcg gagtattggt caaaatgtaa acgcatattg actatactta
     5941 cgccattgag gtaaaaaaca gcgtttcatt cggtgaatgg ataaggcaca atgccggcca
     6001 ccgttttctt tctctggttt cagatgaaag aaaatgggcg aatctggtta acaaaagcgg
     6061 tgcaatatgc aagtttttat catgcgtcac ggcgacgcag ccctcgatgc cgccagtgat
     6121 tcggttcgtc ctctgaccac taatggttgt gacgaatctc gcctgatggc gaactggctg
     6181 aaaggtcaaa aagtggaaat cgaacgcgtt ctggtgagcc cgttcctgcg agccgagcaa
     6241 acactggaag aagtagggga ttgtctgaac ctgccttcca gtgcagaagt tctgccggag
     6301 ttaacgccct gcggcgatgt tggtctggtc agtgcctatt tgcaggcgtt gaccaatgaa
     6361 ggtgttgctt cggtactggt gatctctcac ttaccgttag tcgggtatct ggttgccgag
     6421 ttatgtccgg gcgaaacgcc gcctatgttt accacctcgg ccattgccag cgtaacgctt
     6481 gatgaaagcg gaaacggtac attcaactgg cagatgagcc cgtgtaatct gaagatggca
     6541 aaagctatct gatgtgttcc aaattatgcc caatgaggta gattcattgg gcataattgc
     6601 tcatcaggca gcggatatct gtcaacctca ctactatcga agactctcct tatctataaa
     6661 taaaacactt tctacattca ttcggaaatt attcttctgc acgtatcttg attttgccca
     6721 aagttacatg ttgaaatgaa taatgtctgt ttgacactca ttgtcattta ttaatacaga
     6781 taatagagtt gaataatcag gcaggatgta attgaaactt gattaaattc tggctatagt
     6841 gtttatatat tatattgtca aaagtggata aatgccaatc gcattatatg ttcattgttt
     6901 gttttttaag ctgttgatat tcattattga tatactttct attttttcta aatacttatt
     6961 ccgtattttt taaggatgtc cttaagataa tctttcttct gtcaatatat taattaaatc
     7021 tacatttact ctttagtaaa attaaattgc atcaggggtg tggaaacatc actatgcggc
     7081 cccacaccaa cgaatacaat tttctacgac ctgttctttt tggatcgcat gccgtattca
     7141 atggctgggt ataaacattg gctattgact gaatcaatgt attgcaatgt ttatattttt
     7201 aattatttat agtttaagga caaataatga gtaagtttgt taaaacagct attgctgcgg
     7261 caatggtgat gggcgtgttc acctctacgg caacaatcgc tgcgggtaac aacggtactg
     7321 cacgttttta cggcaccatt gaagactcag tgtgttctat cgttccggac gatcacaaac
     7381 tggaagttga tatgggtgat atcggtgccg aaaaactgaa aaataacggc accaccacgc
     7441 cgaaaagttt ccagattcgt ctgcaagatt gcgtatttga tacttaggaa acaatgacca
     7501 ctacctttac tggtaccgtt tcttctgcaa atagcggcaa ttattacacc attttcaata
     7561 ccgataccgg tgcggcattt aacaatgtta gcctggcgat cggtgactct ctgggtacct
     7621 cttacaaaag cggcatgggt attgaccaga aaatagtgaa agacacttct actaataaag
     7681 gcaaagcgaa gcagacactg aactttaaag cctggctggt cggcgcagct gatgcgccag
     7741 atctgggtaa tttcgaagca aataccacct tccagattac ttacctgtaa ggcatacccc
     7801 catagcgaca ctgctatggg ggatttaaat ggattttgac cgaacaagaa taaaaacgga
     7861 tagaaacgtg tatgcctgac cattctcttt ttcgattacg gattcttccg tggtgcattg
     7921 cgctggcaat gtcagggagt tatagcagtg tctgggctga agacgacatt cagtttgatt
     7981 cccgttttct ggaattaaaa ggcgacacaa aaattgatct gaagcgtttt tccagccagg
     8041 gatatgttga gcccggaaaa tacaatttac aggttcaact aaataaacag ccattggcgg
     8101 aagagtacga tatttactgg tatgctggtg aagatgacgc gagcaaaagc tatgcttgtc
     8161 tgacaccgga actggtagcg cagtttggtt taaaagaaga cgtggcgaaa aatctgcaat
     8221 ggagccacga tgctaaatgc ctgaaatccg gtcaactgga aggcatggaa attaaggctg
     8281 atttaagcca gtccgcatta gtcatttcac tgccacaggc ttacctcgaa tatacttatc
     8341 ccgactggga tccgccttca cgttgggatg acggcatctc cgggatcgtc gcggactaca
     8401 gcatcaacgc acaaacccgg cacgaagaaa atggcggtga tgatagtaac gagatcagcg
     8461 gcaacgggac ggtcggggtt aacctggggc cgtggcgtat gcgtgctgac tggcagacta
     8521 actatcaaca tactcgcagt aatgatgacg atgaattcag cggcgatgaa actcaaaaaa
     8581 aatgggagtg gagtcgctac tatgcctggc gggcgttacc atcattaaaa gccaaactgg
     8641 cgctgggcga ggattacctc agatccgata tttttgatgg ttttaactat gttggtggta
     8701 gtgtcagtac tgacgatcaa atgttgcctc ccaatctgcg cggctacgcg ccagacattt
     8761 ccggcgtggc acacaccaca gcaaaagtga ccgtcagcca gatggggcgt gtgatttacg
     8821 aaacgcaggt tccggctgga ccgtttcgta ttcaggatct tggtgattct gtctccggta
     8881 cgttgcatat tcgcattgaa gaacagaacg gccaggtgca ggaatatgac atcagcaccg
     8941 cctcgatgcc atacctcact cgtccaggtc agattcgcta taagatcatg atgggccgtc
     9001 cgcaagagtg gggacaccat gtcgagggtg aatttttttc tggtgctgaa gcttcctggg
     9061 ggatcgctaa cggctggtcg ttatatggcg gcgcactggg agatgaaaac tatcagtctg
     9121 cggcgcttgg cgtcggtcgc gatttgtcta cattcggcgc ggtcgcgttt gatgttactc
     9181 actcgcacac caaactggat aaagacaccg cttatggcaa aggttcgctg gacggtaact
     9241 ccttccgtgt gagttattcc aaagactttg accagctcaa cagccgcgtt acttttgctg
     9301 gatatcgctt ctcggaagag aactttatga ccatgagcga gtatctggat gccagtgaca
     9361 gcggaatggt acgcacgggc aacgacaaag agatgtgcac cgccacttat aaccagaact
     9421 tccgcgatgc gggtgtttcg gtttatctca actatacccg ccatacctac tgggatcgcg
     9481 aggagcagat aaactacaac atcatgctct cgcactattt caatatgggc agtattcgta
     9541 atgtcagcat ctcgatgact ggctaccgtt acgagtatga caaccaggcc gacaaaggca
     9601 tgtacatttc gctcagtatg ccgtggggcg acaacagtac cgttagctat aacggtaact
     9661 atggcagtgg gacggacagc agtcaggtcg gttatttcag ccgtgtcgat gacgcgactc
     9721 actatcagtt gaacgtcggc accagtgaca aacacaccag cgttgacggc tattacagcc
     9781 atgatggttc gctggcgcag gttgacctca gtgcgaacta ccatgaaggg caatacacct
     9841 ctgcgggctt gtcgttacag ggcggcgcaa cgcttactgc ccacggtggc gcacttcacc
     9901 gtacccagaa tatgggcggg acacgcttgt tgattgatgc cgatggcgtt gccgatgttc
     9961 cggtggaagg taacggggct gctgtttata ccaatatgtt tggtaaagcc gtcgtttctg
    10021 acgtcaataa ctattaccgc aatcaggcgt atatcgacct caacagattg cctgaaaacg
    10081 ctgaagcaac ccagtcggtg gtgcaagcca cgctaactga aggtgccatt ggctaccgta
    10141 aatttgctgt cattagcgga caaaaagcga tggcggtgct gcgcctgagc gacggcagcc
    10201 atcctccgtt tggcgcagaa gtaaaaaatg ataacgagca gacagtgggc cttgtcgatg
    10261 atgacggcaa tgtttatctg gctggggtga aacctggcga acacatgagt gtgttctgga
    10321 gtggtgttgc gcattgcgat atcaacctgc cggacccgct gcctgccgat ctgtttaacg
    10381 gcttgttact accatgccag cataaaggca atgtagcacc tatcacttcg ccggcggtca
    10441 aaccggcgat tcaggaacag acacagcggg tgacgccaac ggaacctccg acttcaattt
    10501 cagtaaacca gtaacgtgat taaggaatga tccatgttta atctgactaa cactgcaaaa
    10561 atcgttgtcc cggcactggc gctgctggcg acagcggtca gtttctccag ccacgcttcg
    10621 gtgacgcccg acaggacacg gctggtgttt aacgaaagcg acaagtccat cagcgtcacg
    10681 ctgcgcaaca acgatcccac actgccttat ctggcgcaga gctggataga ggatgcaaag
    10741 ggcaacaaaa tcagctctcc gcttgcggtg cttccgccag tgcagcgcat cgatgcgatg
    10801 atgaacggcc aggtgaaggt gcaggggctg ccggatatca acaaactgcc ggccgaccgc
    10861 gagagcgtgt tctatttcaa cgtgcgcgaa atttcgccga aatcaaataa accgaacaca
    10921 ctgcagattg cgctgcagac gcgtatcaaa ctgttctggc gaccgaaggc gctggagaac
    10981 accagcatga aaagtccgtg gcagcataag gtgacgctca tccgtaacgg ccaggacttc
    11041 accgtcaata acccgacgcc gtactacgtg atcatcagca acgccagcgc taaaaaaggc
    11101 ggcaacccgc cggcgggatt cacaccgctg atgatggagc cgaaaacctc cgcaccgctg
    11161 aatgcgaaga tggacagcgt gccggtgctg acttacatca acgactacgg cgcgcggatg
    11221 ccgctgtact tcaaatgcac cggcaacagc tgccaggtgg atgaagagca gagccgcaaa
    11281 ggctgatgcc gggagggact tatgagtcac aagtttgtag cgggcgcagg gttgctggtg
    11341 ctggccggat ggggcatcag cggtttggct gttgctggca gtgatatcaa acaggtggat
    11401 ctgacgctgc gaatcctagt cgatggaccg ccgccttgct ccgtgaaggg aagcgctgtg
    11461 aaatttggca atgtgataat caacaggatc gatggtacca attaccaaca ggatgcgaaa
    11521 tacaccctca actgcggtaa taaagtcagc gatgacttac ggatgcagct caaaggcaat
    11581 accgccgtga tcaacggaga aacggttctc agcacggata tcgccggcct gggggtgcgg
    11641 atagaaaacg ccgccgacaa ctccctgttt gcagttggtg aaaataactg gacgcctttt
    11701 aatgtcaaca ctcagcctca gctgaaagcg gtgccggtaa aacagagcgg ggtgcaactg
    11761 actccgtcgg agtttaacgc cacgatgacc atggtggtgg attaccaatg aaaaaattcc
    11821 tgctcctttt actgtgcgct ggttgcgggg ctgtgctggc ggcggaagat gacatcacat
    11881 tccacggtac gctgatgtcg ccgcccgcct gtagcatcag cggcgggcaa accatcgaag
    11941 tgcaatttcg cgatctcatt atcgacagca ttgatggcaa ttatggtcgt cagaaagtgg
    12001 actacgaact gagctgcgac tcggatgtcc gcgatcccga gtggaatatg acactctcct
    12061 ggacggggac tgccacctcc tacaacgact ctgctattga gacggatgta ccgggcttcg
    12121 gaattgagct tcagcatgac ggtcagcgtt ttaagctcaa cgagccgctt tccattaacg
    12181 ccaccgattt tagccagaag ccaaagctgg aggcggtacc ggtcaaagcg gctgatgcgg
    12241 tgctgacgga cacgaccttt tctgcttacg caaccttacg ggtggattac caatgaaaac
    12301 agcacacatt tattcacccg gttatgcgct gttagcaggc ctgctgctga ccgccagcac
    12361 aggagcacag gcagtcgaca acaacctgca tttttacggc aacctgctga gtaaatcctg
    12421 cacgctggtg gtggatggtg ccaacctggc agaaatccat tttcccaccg tcagcaaccg
    12481 ggatttgatg gtatctgggc agtccgcgca tctgccggtt gtgtttaagc tgaaagactg
    12541 caaagggccg gcgggataca acgtcaaagt cacgctgagc ggcaccgaag acagcggtca
    12601 gccgggtttt ctggcagtcg atgacacctc caccgcgcag ggcgtgggga tagggatgga
    12661 aacgctcgac ggcaagcggg tggatattaa caacaccacc ggggcgacct ttgcgcttac
    12721 caacggcaac aatgatatta atttccgggc ctggatctag gcgaaaagcg gtcgtgatgt
    12781 aaccatcggt gatttcaccg ccagcctgac agcaactttt gagtatattt aaaatgaaaa
    12841 tccttcgtca tgttttatgt cttttattgc tgttaatgtt caatacatgc ggccaggcgg
    12901 cgacgaaagt tggtgagtta tatgtgccag ctggtgccag tt
//
LOCUS       DAJVUJ010000120        12914 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.120, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000120 DAJVUJ010000000
VERSION     DAJVUJ010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12914
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.120"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(<1..880)
                     /gene="rarD"
                     /locus_tag="OQQ48_003036"
     CDS             complement(<1..880)
                     /gene="rarD"
                     /locus_tag="OQQ48_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HCS1953724.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMLLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRR"
     gene            complement(932..1399)
                     /locus_tag="OQQ48_003037"
     CDS             complement(932..1399)
                     /locus_tag="OQQ48_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HCS1953725.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            1564..2433
                     /gene="pldA"
                     /locus_tag="OQQ48_003038"
     CDS             1564..2433
                     /gene="pldA"
                     /locus_tag="OQQ48_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HCS1953726.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2566..4395
                     /gene="recQ"
                     /locus_tag="OQQ48_003039"
     CDS             2566..4395
                     /gene="recQ"
                     /locus_tag="OQQ48_003039"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCS1953727.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMCKLERFGKPFMALIRAHVDGDDEE"
     gene            4459..5079
                     /gene="rhtC"
                     /locus_tag="OQQ48_003040"
     CDS             4459..5079
                     /gene="rhtC"
                     /locus_tag="OQQ48_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HCS1953728.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQIELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5141..5761)
                     /gene="rhtB"
                     /locus_tag="OQQ48_003041"
     CDS             complement(5141..5761)
                     /gene="rhtB"
                     /locus_tag="OQQ48_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HCS1953729.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            5872..6894
                     /gene="pldB"
                     /locus_tag="OQQ48_003042"
     CDS             5872..6894
                     /gene="pldB"
                     /locus_tag="OQQ48_003042"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HCS1953730.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            6902..7702
                     /gene="yigL"
                     /locus_tag="OQQ48_003043"
     CDS             6902..7702
                     /gene="yigL"
                     /locus_tag="OQQ48_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HCS1953731.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            7778..8677
                     /gene="bioP"
                     /locus_tag="OQQ48_003044"
     CDS             7778..8677
                     /gene="bioP"
                     /locus_tag="OQQ48_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HCS1953732.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMNKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTLITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(8565..9518)
                     /gene="metR"
                     /locus_tag="OQQ48_003045"
     CDS             complement(8565..9518)
                     /gene="metR"
                     /locus_tag="OQQ48_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HCS1953733.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            9754..12015
                     /gene="metE"
                     /locus_tag="OQQ48_003046"
     CDS             9754..12015
                     /gene="metE"
                     /locus_tag="OQQ48_003046"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCS1953734.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLAVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPARHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTWLWLGKVKGEQFDRLSLLNDILPVYQQVLAELEKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDGVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(12300..12545)
                     /locus_tag="OQQ48_003047"
     CDS             complement(12300..12545)
                     /locus_tag="OQQ48_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCS1953735.1"
                     /translation="MITETEINVLKVMAEKDINWNWMNLDRTLSIRQIPGFGNVVNIV
                     NKLANEGLVIIEDSGHKSMPHYHVTEKGYNFVKSENK"
     gene            complement(12535..>12914)
                     /locus_tag="OQQ48_003048"
     CDS             complement(12535..>12914)
                     /locus_tag="OQQ48_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1953736.1"
                     /translation="FNPDLAGGPIENLTTDGVTINREGIAIVEKHIARFGHDPINEVM
                     INRLKDIEKGKIPPEQVDLNFYTHECREYQRYCNLGWETGQPDGDAGYALWNHTHTAT
                     LEDYKLKGELNDLYHQDALDYDN"
BASE COUNT         3051 a         3261 c         3442 g         3160 t
ORIGIN      
        1 ttctacgctg cgtataaatc gcatccatca caaaaattgc cagcgccacc caaataaagg
       61 cgaaggtcac catcttatcg gcacccggtt tttcaccata aaacgtcact gccaacagga
      121 acatcagcgt cgggccaata tactggaaga agcctaacgt ggagagacgc aggcgtgtag
      181 cagctgcggt aaaacacaac agcggtacgg tggtgacaat accggcggcg atcagcagca
      241 aattcagcaa catcgggttt tgccccatat ggctggttga gctgtcggca atagcaaaca
      301 gataaatcgc cgccacgggc agcagccaca tggtttcgat taacatgccg gtttgcgctt
      361 caacggcaat tttcttgcgt accagtccgt agaaggcaaa actaaacgcc agccccagcg
      421 cgatgattgg cagcgaacca aaagtccaca actgaaccaa cacgccacat atcgccagaa
      481 tcaccgccag ccattgcatc cggcggaatc gctcgccgag gaaaatcatc cccagcacaa
      541 tgttcaccag cgggttaata aagtaaccaa ggctcgcttc cagcatatgg tgattgttca
      601 ccgcccagat aaacagtagc cagttgccac caatcagcac ggcagagact gccagcataa
      661 aaattttctg tggcgtctga atcagcgttt ttaaatagga ccactggcgg caaatgctca
      721 tcagcaccac cataaagaaa aacgaccaga tcacgcgatg cgtcaggatc tcatcggcgg
      781 gcacgtagta aatcagcttg aagtatgctg gcgcaatacc ccaaataaaa taagcggcaa
      841 gagcgagtaa tacgccctgc cgcgtttgtt ttgcatccat cgggaatact catttttaat
      901 tggtaacagc agtttacctg cttttatgtc ttcaacctac catataggtg gcggtggcac
      961 tggcaatata aagctgttct tcattgtgta attcaacgcg ggcgacggcg actttattgc
     1021 ctgcacgcaa caggctactg gtagcagtaa aacgctcgcc cctgcctggg cgcagataat
     1081 caacgcgaag atcaatggtc cccatccgcg atagccgctg gcgtagttca tcttcactga
     1141 tggtttcgtg gcgggttaag gtacttccca cgcacaccag accggcggcg acatccagcg
     1201 ccgacgcaat gactccgccg tgcaaaatgc tttgcgccca gttacccacc atcatcggct
     1261 gatttttaaa ggccagctgt gcgaactctt tttcgtaacg ctccagctcc atccctaacg
     1321 cgcggttaaa tggcatgtga taaacaaaca tctcacccac taatttcagg gcttgttcag
     1381 cggtcagtac ggcagacata tcatccttac acttcattgg ttaatgaaat gttgatttta
     1441 tgcttctttg ttgttggttt ctactttagg aagggataac taacggcaat ggagttaagt
     1501 atgtaaaata gcccgcagaa aaacattcac cttatcaata attcgttacg gagaacacga
     1561 ccgatgcgga ctctgcaggg ctggttgttg ccggtgttta tgttgcctat ggcagtatat
     1621 gcacaagagg caacggtgaa agaggtgcat gacgcgccag cggtgcgtgg cagtattatc
     1681 gccaatatgc tgcaggagca tgacaatccg ttcacgctct atccttatga caccaactac
     1741 ctcatctaca cccaaaccag cgatctgaac aaagaagcga ttgccagtta cgactgggcg
     1801 gaaaatgcgc gtaaggatga agtaaagttt cagttgagcc tggcatttcc actgtggcgt
     1861 gggattttag gcccgaactc ggtgttgggt gcgtcttata cgcaaaaatc ctggtggcaa
     1921 ctgtccaata gcgaagagtc ttcaccgttt cgtgaaacca actacgaacc gcaattgttc
     1981 ctcggttttg ccaccgatta ccgttttgca ggttggacgc tgcgcgatgt ggagatgggg
     2041 tataaccacg actctaacgg gcgttccgac ccgacctccc gcagctggaa ccgcctttat
     2101 actcgcctga tggcagaaaa cggtaactgg ctggtagaag tgaagccgtg gtatgtggtg
     2161 ggtaatactg acgataaccc ggatatcacc aaatatatgg gttactacca gcttaaaatc
     2221 ggctatcacc tcggtgatgc ggtgttgagt gcgaaaggac agtacaactg gaataccggt
     2281 tacggcggcg cggagttagg cttaagttac ccgatcacca aacatgtgcg cctttatact
     2341 caggtttaca gcggctatgg cgaatcgctc atcgactata acttcaacca gacccgtgtc
     2401 ggtgtaggag ttatgctaaa cgacttgttt tgatgaacgg ttgagtggtt ggcaaatctg
     2461 gaacccagca tccaggatta ccctctcaga gactaaaagc attgcagttt ctcgcgcagg
     2521 cgctgaaaat agcgcctgtt tttatttcag gcaatcgggg tgaatgtggc gcaggcggaa
     2581 gtgttgaatc tggagtccgg agctaaacag gttttacaag aaacctttgg ctaccaacag
     2641 tttcgccccg gccaggaaga aattatcgac actgtgcttt ccggtcgcga ttgcctggtc
     2701 gtcatgccca ccggtggcgg aaaatccctt tgctatcaaa ttcctgcctt attgctaaac
     2761 ggccttaccg tggttgtttc accgctgatt tcgttgatga aagatcaggt ggatcaactg
     2821 caagccaacg gcgtggcggc ggcgtgcctt aactcgacgc aaacccgcga gcagcaactt
     2881 gaagtgatga caggctgccg caccgggcaa attcgtctgc tttatatcgc cccggaacgc
     2941 ctgatgctgg ataactttct tgagcatctg gcgcactgga atccggtgtt attagccgtt
     3001 gatgaagcgc actgtatctc ccaatggggc cacgatttcc gcccggaata tgccgcgctt
     3061 ggtcagttac gccagcgttt cccgaccctg ccgtttatgg cgctgactgc cacagccgac
     3121 gacaccacgc gccaggatat cgtgcgcttg ctggggctga acgatccgct gattcaaatc
     3181 agcagttttg accgtccgaa tattcgctac atgctgatgg agaagttcaa accgctcgat
     3241 cagttgatgc gctacgtgca ggaacagcgc ggtaagtcag gcattatcta ctgcaacagc
     3301 cgcgcgaaag tagaagacac cgctgcgcgc ctgcaaagca agggaattag cgcggcggcc
     3361 tatcatgctg ggctggaaaa taatgttcgc gccgacgtgc aggaaaaatt tcagcgcgat
     3421 gacctgcaaa ttgtggtggc gacggtggcg ttcggcatgg gcatcaataa accaaacgtt
     3481 cgcttcgtgg tccactttga tattccgcgc aatatcgaat cctattatca ggaaaccggt
     3541 cgcgccgggc gtgatggcct gcccgcggaa gcgatgctgt tttacgatcc ggccgatatg
     3601 gcgtggctgc gccgttgcct ggaagagaag ccgcaggggc agttacagga tatcgaacgt
     3661 cataaactca atgcgatggg cgcttttgcc gaagcgcaaa cttgccgtcg tctggtattg
     3721 ctgaactatt ttggcgaagg gcggcaagag ccgtgcggga actgcgatat ctgcctcgat
     3781 ccgccgaaac agtacgacgg ttcaaccgat gctcagattg ccctttccac cattggtcgt
     3841 gtgaatcagc ggtttgggat gggttatgtg gtggaagtga ttcgtggtgc taataaccag
     3901 cgtatccgcg actatggtca tgacaaactg aaagtctatg gcatgggccg tgataaaagc
     3961 catgaacatt gggtgagcgt gatccgccag ctgattcacc tcggcctggt gacgcaaaat
     4021 attgcccagc attctgccct acaactgaca gaggccgcgc gcccggtgct gcgcggcgaa
     4081 tcctctttgc aacttgccgt gccgcgtatc gtggcgctca aaccgaaagc gatgcagaaa
     4141 tcgttcggcg gcaactatga tcgcaaactg ttcgccaaat tacgcaaact gcgtaaatcg
     4201 attgccgatg aaagtaatgt cccgccgtac gtggtgttta acgacgcaac cttgattgag
     4261 atggctgaac agatgccgat caccgccagc gaaatgctca gcgttaacgg cgttgggatg
     4321 tgcaagctgg aacgctttgg taaaccgttt atggcgctga ttcgtgcgca tgtcgatggc
     4381 gacgacgaag agtagtcagc agcataaaaa agtgccagga tgaagactcc gtaaacgttt
     4441 cccccgcgag tcaaatgtat gttgatgtta tttctcaccg tcgccatggt gcacattgtg
     4501 gcgcttatga gccccggtcc cgatttcttt tttgtctctc agaccgctgt cagtcgttcc
     4561 cgtaaagaag cgatgatggg cgtgctgggt attacctgcg gcgttatggt ttgggcaggg
     4621 attgcgttgc ttggcctgca tttgattatc gaaaaaatgg cctggctgca tacgctgatt
     4681 atggttggcg gtggcctgta tctctgctgg atgggttacc agatgctacg cggtgcactg
     4741 aaaaaagagg ctgtatctgc gcctgcacca cagattgagc tggcgaaaag cgggcgcagt
     4801 ttcctgaaag gtttactgac caatctcgct aatccgaaag cgattatcta ctttggctcg
     4861 gtgttctcat tgtttgtcgg tgataacgtt ggcactaccg cgcgctgggg catttttgcg
     4921 ctgatcattg tcgaaacact ggcgtggttt accgtcgttg ccagcctgtt tgccctgccg
     4981 caaatgcgcc gtggttatca acgtctggcg aagtggattg atggttttgc cggggcgtta
     5041 tttgccggat ttggcattca tttgattatt tcgcggtgat gccagacgcg tcttcagagt
     5101 aagtcggata aggcgtttac gccgcatccg acattatttt tcacgcatgc ctcgccgatg
     5161 ctaacagcgc tcccaccagc ataaacaacg agccgaaaat cttattcagc gccttcatct
     5221 gctttggtcc tttaatccat agagcaatcc gttgagcaag ggtggcgtaa ccgatcatca
     5281 caataatatc gactacaata gtggtgacgc cgagcacgat atactgcatc agttgcggct
     5341 gctgtggcat gatgaattgc ggaaatagcg ccgccagaaa cacaatactt ttgggattgg
     5401 tgagattcac aaaaactgcg cgctggaaca aatgtcgacg cgattgagta gaggccagcg
     5461 atttaaggtc aattgcacca gcggcgcgcc actgctggat tcccagccaa atcaagtaag
     5521 ccgcgcctgc ccacttcaac acttcaaacg caatcactga gcgggaaaat agcgtcccca
     5581 aacccacgcc aaccagcaca atatgaatcg ccagtccggt ctgaagccca gcaatagacg
     5641 ccaccgcgcc gcgataaccg tggttgagcg aggtggtcat agtgttgatt gcaccagagc
     5701 ctggcgacag gcttaaaatg atcgatgtca gcaggtaggc aaaccaccat tctaaggtca
     5761 tgatgaactc ccggtgtgtc tatttttgtg ccacaatacg ctactgtcgc agcgttgtgt
     5821 caggcacgct aaaaaaaacg attttacgtg gtttaagagg cagattaccc gatgtttcag
     5881 cagcaaaaag actgggaaac aagagaaaac gcgtttgctg cttttaccat gggaccgctg
     5941 actgatttct ggcgtcagcg tgatgaagca gagtttactg gtgtggatga cattccggtg
     6001 cgctttgtcc gttttcgcgc acagcaccat gaccgggtgg tagtcatctg cccggggcgt
     6061 attgagagct acgtaaaata tgcggaactg gcctatgacc tgttccattt ggggtttgat
     6121 gtcttaatca tcgaccatcg cgggcaggga cgttccggtc gcctgttagc cgatccgcat
     6181 ctcgggcatg ttaatcgctt taatgattat gttgatgatc tggcggcatt ctggcagcag
     6241 gaggtccagc ccggtccgtg gcgtaaacgc tatatactgg cacattcgat gggcggtgcg
     6301 atctccacat tatttctgca acgccatcca ggtgtatgtg acgccattgc gctaactgcg
     6361 ccaatgtttg ggatcgtgat tcgtatgccg tcatttatgg cacggcagat cctcaactgg
     6421 gccgaagcgc atccacgttt ccgtgatggc tatgcaatag gcaccgggcg ctggcgcgcg
     6481 ttgccgtttg ctatcaacgt actgacccac agcagacagc gatatcgacg taacttacgc
     6541 ttctatgctg atgacccaac gattcgcgtc ggtgggccga cctaccattg ggtacgcgaa
     6601 agtattctgg ctggcgaaca ggtgttagcc ggtgcgggtg atgacgccac gccaacgctt
     6661 ctcttgcagg ctgaagagga acgcgtggtg gataaccgca tgcatgaccg tttttgtgaa
     6721 ctccgcaccg ccgcgggcca tcctgtcgaa ggaggacggc cgttggtaat taaaggtgct
     6781 taccatgaga tcctttttga aaaggacgca atgcgctcag tcgcgctcca cgccatcgtt
     6841 gattttttca acaggcataa ctcacccagc ggaaaccgct ctacagaggt ttaaatttct
     6901 tatgtaccag gttgttgcgt ctgatttaga tggcacgtta ctttctcccg accatacgtt
     6961 atccccttac gccaaagaaa ctctgaagct gctcaccgcg cgcggcatca actttgtgtt
     7021 tgcgaccggt cgtcaccacg ttgatgtggg gcaaattcgc gataatctgg agattaagtc
     7081 ttacatgatt acctccaatg gtgcgcgcgt tcacgatctg gatggtaatc tgatttttgc
     7141 tcataacctg gatcgcgaca ttgccagcga tctgtttggc gtagtcaacg acaatccgga
     7201 catcattact aacgtttatc gcgacgacga atggtttatg aatcgccatc gcccggaaga
     7261 gatgcgcttt tttaaagaag cggtgttcaa atatgcgctg tatgagcctg gattactgga
     7321 gccggaaggc gtcagcaaag tgttcttcac ctgcgattcc catgaacaac tgctgccgct
     7381 ggagcaggcg attaacgctc gttggggcga tcgcgtcaac gtcagtttct ctaccttaac
     7441 ctgtctggaa gtgatggcgg gcggcgtttc aaaaggccat gcgctggaag cggtggcgaa
     7501 gaaactgggc tacagcctga aggattgtat tgcgtttggt gacgggatga acgacgccga
     7561 aatgctgtcg atggcgggga aaggctgcat tatgggcagt gcgcaccagc gtctgaaaga
     7621 ccttcatccc gagctggaag tgattggtac taatgccgac gacgcggtgc cgcattatct
     7681 gcgtaaactc tatttatcgt aatcgttctt tatttggtca gttgtcaacc tgatacttcg
     7741 ctacaatgga tactcgttaa tcaaagagtt ttccattgtg gcgctactta tcatcaccac
     7801 gattctgtgg gccttctcct ttagctttta tggcgagtac cttgcggggc acgtcgatag
     7861 ctattttgcg gtgctggtgc gcgttggcct ggcggcactc gtttttctgc cgtttctgcg
     7921 tacccgtggc aatagcctga aaacagtcgg cctgtatatg ctggtgggcg cgatgcagct
     7981 cggcgtgatg tatatgctga gtttccgcgc ttatctctat ctgacggttt ccgaactgct
     8041 gctgttcacc gtactgacgc cgctctacat cacgctgatt tatgacatca tgaataagcg
     8101 ccgtctgcgc tggggatatg cttttagtgc cttgttggcg gtgattggtg ccgggattat
     8161 tcgctatgat caggtcaccg accatttctg gactggcttg ttgctggtgc aactctccaa
     8221 tatcactttt gccatcggca tggtgggtta caaacgcctg atggaaactc gcccgatgcc
     8281 gcagcataac gcctttgcgt ggttctatct tggcgcgttc ctggtggcgg taattgcatg
     8341 gttcttgctg ggaaatgcgc agaaaatgcc gcaaaccacg ttgcaatggg gcattctggt
     8401 gtttcttggc gtggtggctt ccgggattgg ctactttatg tggaactacg gcgcgacgca
     8461 ggtggacgcc ggaacgctgg gaattatgaa taatatgcac gttccggcag ggctgctggt
     8521 aaacctggct atctggcacc aacagccgca ctggccaacg ttgattacag gcgcgctggt
     8581 gatcctggcc tcactgtggg tgcatcgtaa gtgggtcgct ccgcgctctt cacaaacggc
     8641 agatgatcgc aggcgtgatt gcgcgctgag cgaataaacg cttccgtaac tggctgacgc
     8701 tgctcgccat cgcgcacggc ggcgtacagt cggctccaca agccttcgcc cagggttttg
     8761 gtcaccacca gaccctggcg ctcaaaactc tctactaccc aatgcggtag cgcggcaata
     8821 cccatccgcg cggcaaccat ctgaatcaac aataaggtgt tatcgacgct tttcagtgac
     8881 ggactgacgc ctgccggctg aagaaaatgc cgccagacat ccagtcgact acgctgcacc
     8941 ggataaatta atagcgtctc gctggcgaga tcttccggtg taatacgcgt ttttgccgcc
     9001 agtggatggt caggtgctaa caccagacgc acttcatagt cgaacatcgg cgaataatgc
     9061 agaccactgc gcggcagaat atcggacgtc attaccagat ccagctctcc ctgttgcaag
     9121 gcgggctgcg ggtcaaatgt cacgcccgat ttaaagtcca tctcgacctg cggccagttc
     9181 ttatggaaat tttctaacgc gggcgtcagc cactgtatac agctatggca ctcaatggca
     9241 atgcgcagac gcgtctgctg cggttcattg caggcttgca gtgcctggct aatttgtggc
     9301 agcacctggt ttgccagttg caacaggatt tctccctgcg gtgtaaagcg aagcggctgg
     9361 cttttacgca caaatagccg gaagccaagg cgttgttcca gatcgctaaa ctggtgagac
     9421 agggcggatt gcgtttgatg caacgtcgcc gcagcggctg cgagtgagcc gcagttccgc
     9481 aacgcttgta gcgttttcag atgttttact tcgatcatga aagtccttca cttcggcatg
     9541 aataatttgc gcttgaggaa tatacagtaa ccgccaatta tggatgtgta aacatctgga
     9601 cggctaaaat ccttcgtctt ttaaatttat ggtgcgttgg ctgcgtttct ccaccccggt
     9661 cacttacttc agtaagctcc cggggatgaa taaacttgcc gccttcccta aattcaaaat
     9721 ccataggatt tacatataat tagaggaaga aaaatgacaa tattgaatca caccctcggt
     9781 ttccctcgcg ttggcctgcg tcgcgagctg aaaaaagcgc aagagagtta ttgggcaggg
     9841 aactccacgc gtgaagaact gctggcggta gggcgtgaat tgcgtgctcg tcactgggat
     9901 caacaaaagc aagcgggtat cgacctgctg ccggtgggcg attttgcctg gtacgatcat
     9961 gtactgacca ccagtctgct gctgggtaac gttccggcgc gtcatcagaa caaagatggt
    10021 tcggtagata ttgacaccct gttccgtatt ggtcgtggac gtgcgccgac tggcgaacct
    10081 gcggcggcag cggaaatgac caaatggttt aacaccaact atcactacat ggtgccggaa
    10141 ttcgttaaag gccaacagtt caaactgacc tggacgcagc tgctggagga agtggacgag
    10201 gcgctggcgc tgggccacaa cgttaagcct gtgctgctgg ggccggttac ctggctgtgg
    10261 ctggggaaag tgaaaggtga acagtttgac cgcctgagcc tgctgaacga cattctgccg
    10321 gtttatcagc aagtgctggc agaactggag aaacgcggca tcgagtgggt acagattgat
    10381 gaacccgcgc tggtactgga actaccacag gcgtggctgg acgcatacaa acccgcttac
    10441 gacgcgctcc agggacaggt gaaactgctg ctgactacct attttgaagg cgttacgcca
    10501 aatctcgaca cgattactgc gctgcctgtt cagggactgc atgtggacct tgtacatggt
    10561 aaagatggcg ttgctgaact gcacaagcgc ctgccttctg actggctgct gtctgcggga
    10621 ctgatcaatg gtcgtaacgt ctggcgcgcc gatcttactg agaaatatgc gcaaattaag
    10681 gacattgtcg gcaaacgtga tttgtgggtg gcatcttcct gctcgttgct gcacagcccc
    10741 atcgacctga gcgtggaaac gcgtcttgat gcagaagtga aaagctggtt tgccttcgcc
    10801 ttacaaaaat gccatgaact ggcactgctg cgcgatgcgc tgaacagtgg tgacacggca
    10861 gctctggcag agtggagcgc cccaattcag gcgcgtcgtc actctactcg tgtacataat
    10921 ccggcggtag aaaagcgtct ggcggcgatc accgcccagg acagccagcg tgcgaatgtc
    10981 tatgaagtgc gtgctgaagc ccagcgtgcg cgttttaaac tgccagcgtg gccgaccacc
    11041 acgattggtt ccttcccgca aaccacggaa attcgtaccc tgcgtctgga tttcaaaaag
    11101 ggcaatctcg acgccaacaa ctaccgcacg ggcattgcgg aacatatcaa gcaggccatt
    11161 gttgagcagg aacgtttggg actggatgtg ctggtacatg gcgaggccga gcgtaatgac
    11221 atggtggaat actttggcga gcacctcgac ggatttgtct ttacgcaaaa cggttgggta
    11281 cagagctacg gttcccgctg cgtgaagcca ccgattgtta ttggtgacgt tagtcgcccg
    11341 gcaccgatta ccgtggagtg ggcgaagtat gcgcaatctc tgactgataa accggtgaaa
    11401 gggatgctga ctggcccggt gaccattctc tgctggtcgt tcccgcgtga agatgtcagc
    11461 cgtgaaacca tcgccaaaca aattgcgctg gcgctgcgtg atgaagtggc ggacctggaa
    11521 gccgcaggaa ttggcatcat ccagattgat gaaccggcgc tgcgcgaagg tttaccgctg
    11581 cgtcgtagcg actgggatgc atatctccag tggggcgtgg aggccttccg tatcaacgcc
    11641 gccgtggcga aagatgacac acaaatccac actcacatgt gttattgcga gttcaacgac
    11701 atcatggatt ccattgcggc actggatgca gacgtcatca ccatcgaaac ctcgcgttcc
    11761 gacatggagt tgctggagtc gtttgaagag tttgattatc cgaatgaaat cggtccaggc
    11821 gtctatgaca ttcactcgcc aaacgtaccg agcgtagaat ggattgaagc cttgctgaag
    11881 aaagcggcaa aacgcattcc ggcagagcgc ctgtgggtca acccggactg tggcctgaaa
    11941 acgcgcggct ggccagaaac ccgcgcggca ctggcgaaca tggtgcaggc ggcgcagaat
    12001 ttgcgtcggg gatgatgaat attaacgagt cactgtaaat tgcagtacga tgaaagaggc
    12061 aaaactgcct gataccaggc ctacataatt tatgcaattt attgaatttt tgagttttgt
    12121 tggccggaca aggcgttcac gccgcatccg gcatgaacaa cgcgcacttt gccaataatc
    12181 tgatggcttc catcaccgga agccattttg ctatctgcgc ccgttcagat acagaaataa
    12241 ccgaatcatt tttacgcaaa atataatcct gagcgatttg ttcagtaatc aacgctacgt
    12301 tatttattct cacttttcac gaaattgtat cccttttcag taacgtggta gtgaggcata
    12361 gacttatgtc cgctatcctc aataataacc aaaccctcgt tagctaattt gttaacaatg
    12421 ttaacaacat taccgaatcc tggaatttgc cttatggata gtgtgcgatc gagattcatc
    12481 cagttccagt ttatatcttt ctcagccata actttcagta cgttaatttc agtttcagtt
    12541 atcatagtca agtgcatcct ggtggtataa gtcattaagt tctcccttaa gtttataatc
    12601 ttctagagta gcagtatgcg tatgattcca gagtgcatag cctgcgtcac catcaggttg
    12661 tcctgtttcc caaccaagat tacagtaacg ctgatattcc cgacactcat gagtatagaa
    12721 gttaagatct acttgttctg gggggatttt tcctttctca atgtctttca gtctattgat
    12781 catcacttca tttatagggt catgaccaaa tctggctatg tgtttttcaa caatagcgat
    12841 accttccctg ttgattgtta cgccatctgt cgtcagattt tctattggac caccggcaag
    12901 gtcgggatta aatg
//
LOCUS       DAJVUJ010000121        12910 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.121, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000121 DAJVUJ010000000
VERSION     DAJVUJ010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12910
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.121"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(77..1699)
                     /gene="eutB"
                     /locus_tag="OQQ48_003049"
     CDS             complement(77..1699)
                     /gene="eutB"
                     /locus_tag="OQQ48_003049"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HCS1953737.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAYEDDCVT
                     RLIQDDVNETAYNQIKNWSISELREYVLSDETSVDDIAFTRKGLTSEVVAAVAKICSN
                     ADLIYGAKKMPVIKKANTTIGIPGTFSARLQPNDTRDDVQSIAAQIYEGLSFGVGDAV
                     IGVNPVTDDVENLSRVLDTIYGVIDKFNIPTQGCVLAHVTTQIEAIRRGAPGGLIFQS
                     ICGSEKGLKEFGVELAMLDEARAVGAEFNRIAGENCLYFETGQGSALSAGANFGADQV
                     TMEARNYGLARHYDPFIVNTVVGFIGPEYLYNDRQIIRAGLEDHFMGKLSGISMGCDC
                     CYTNHADADQNLNENLMILLATAGCNYIMGMPLGDDIMLNYQTTAFHDTATVRQLLNL
                     RPSPEFERWLESMGIMANGRLTKRAGDPSLFF"
     gene            complement(1689..2525)
                     /gene="eutJ"
                     /locus_tag="OQQ48_003050"
     CDS             complement(1689..2525)
                     /gene="eutJ"
                     /locus_tag="OQQ48_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HCS1953738.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVAELLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGEEIWPTVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            complement(2536..3939)
                     /locus_tag="OQQ48_003051"
     CDS             complement(2536..3939)
                     /locus_tag="OQQ48_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase EutE"
                     /protein_id="HCS1953739.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            complement(3951..4238)
                     /gene="eutN"
                     /locus_tag="OQQ48_003052"
     CDS             complement(3951..4238)
                     /gene="eutN"
                     /locus_tag="OQQ48_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HCS1953740.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            complement(4345..4638)
                     /gene="eutM"
                     /locus_tag="OQQ48_003053"
     CDS             complement(4345..4638)
                     /gene="eutM"
                     /locus_tag="OQQ48_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HCS1953741.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            complement(4677..5693)
                     /gene="pta"
                     /locus_tag="OQQ48_003054"
     CDS             complement(4677..5693)
                     /gene="pta"
                     /locus_tag="OQQ48_003054"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCS1953742.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSFIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YNIAQRLGGYRAVGPLIQGLAVPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            complement(5683..6492)
                     /gene="eutT"
                     /locus_tag="OQQ48_003055"
     CDS             complement(5683..6492)
                     /gene="eutT"
                     /locus_tag="OQQ48_003055"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001425897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HCS1953743.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQKPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVLEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKQPLTVKQIQQRLGERNDY"
     gene            complement(6489..7190)
                     /gene="eutQ"
                     /locus_tag="OQQ48_003056"
     CDS             complement(6489..7190)
                     /gene="eutQ"
                     /locus_tag="OQQ48_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HCS1953744.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAEPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGVLHVRHEGQTMIAKAGDVMFIPKGSSIEFGMTSS
                     VKFLYVAWPANWQSL"
     gene            complement(7165..7644)
                     /gene="eutP"
                     /locus_tag="OQQ48_003057"
     CDS             complement(7165..7644)
                     /gene="eutP"
                     /locus_tag="OQQ48_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HCS1953745.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(7657..7992)
                     /gene="eutS"
                     /locus_tag="OQQ48_003058"
     CDS             complement(7657..7992)
                     /gene="eutS"
                     /locus_tag="OQQ48_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HCS1953746.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLISHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(8284..10563)
                     /gene="maeB"
                     /locus_tag="OQQ48_003059"
     CDS             complement(8284..10563)
                     /gene="maeB"
                     /locus_tag="OQQ48_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HCS1953747.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            10852..11802
                     /gene="tal"
                     /locus_tag="OQQ48_003060"
     CDS             10852..11802
                     /gene="tal"
                     /locus_tag="OQQ48_003060"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCS1953748.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGISAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMAPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            11822..>12910
                     /locus_tag="OQQ48_003061"
     CDS             11822..>12910
                     /locus_tag="OQQ48_003061"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416960.1"
                     /note="catalyzes the formation of ribose 5-phosphate and
                     xylulose 5-phosphate from sedoheptulose 7-phosphate and
                     glyceraldehyde 3-phosphate; can transfer ketol groups
                     between several groups; in Escherichia coli there are two
                     tkt genes, tktA expressed during exponential growth and
                     the tktB during stationary phase; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCS1953749.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTGYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNT"
BASE COUNT         2876 a         3696 c         3358 g         2980 t
ORIGIN      
        1 tgtcccattg acgccatcac gctgcgtaca atttcttcaa tctgtttttg atccatgatg
       61 tgttatctcc gcgtcatcag aagaacagtg acggatcgcc cgcccgtttg gtcaggcgac
      121 cgtttgccat aatgcccatg ctttccagcc agcgttcaaa ctctggtgac gggcgcaggt
      181 tgagtaactg acgcacagtg gcggtatcgt ggaatgcggt ggtctggtag ttgagcatga
      241 tgtcatcacc cagcggcatc cccatgatgt agttgcagcc tgcggtggcg agcaggatca
      301 tcaggttttc gttgaggttc tggtcagcgt cagcgtggtt ggtgtagcag cagtcacagc
      361 ccatagagat gccgctcagc ttgcccataa agtgatcttc taagccagca cggataatct
      421 ggcggtcgtt gtagagatac tccggcccaa taaagccgac cacggtgttg acgataaacg
      481 gatcgtaatg acgcgccagc ccatagttac gtgcttccat cgtcacctgg tcagcgccga
      541 agttagcgcc agcggatagc gcagagcctt gtccggtttc gaagtagagg cagttttccc
      601 cggcgatacg gttgaactct gcacccactg cgcgcgcttc gtcgagcatc gccagttcca
      661 cgccaaactc tttcagccct ttttcgctgc cacagatact ctggaaaatc agtccgccag
      721 gcgcgccgcg acgaatcgct tcgatctggg tggtgacgtg cgccagtacg cagccctgag
      781 ttgggatgtt gaatttgtcg atcacgccat aaatggtatc cagcacgcgg cttaagtttt
      841 ccacgtcgtc agttaccggg ttgacgccga tcaccgcatc gcccaccccg aaggaaagcc
      901 cttcgtagat ttgcgcggcg atactttgca cgtcgtcacg agtgtcattt ggctgcaaac
      961 gggcgctgaa ggtgcccgga ataccgatgg tggtattggc ctttttgatt accggcattt
     1021 tcttcgcgcc gtagatcagg tccgcgttgg agcaaatctt cgctaccgcc gcgaccactt
     1081 ccgaggtcag ccctttgcgg gtaaaggcaa tgtcgtccac gctagtttca tcgctcagca
     1141 catactcacg cagttcgctg atgctccagt ttttaatctg gttgtaggcc gtttcgttaa
     1201 catcgtcctg aatcagccgc gtcacgcagt catcttcata ggcaatcagc ggcaagcgcg
     1261 gttgcagaac gctggtttct gacatctctg ccagcgtgct atccgggttc gtcaccagca
     1321 acgccacggc tttcgccgca tccagcaccg agccgccgcc aaatgcgatc accccatcac
     1381 agcctgactc acgcaactgc gccacggctg cacacacgtc ggtgatgcac ggttcgccca
     1441 ccggacatgg ccagagcgtc atggcgatgc ctttaacagc caggctgcgc gtcagcccgg
     1501 cggtcatccc tgcctgatgc aaaaagctgt ctgccatcac gaacagatgt ttcagcccac
     1561 gcgtttgcgc ttgctgtccg caactgctca ccgagcccgg accgcaaagc gtcaccggtg
     1621 gaacgctaaa tgtttttacc cgttgcagat tcagggtatc gaacgcctga aagagcgcgg
     1681 tctgcaattc attttgcata tagcccctcc gctttctctc gcccactact ggcgatcgcc
     1741 agcggggtca taaacaaact gtgctgcggt aaatgcacct gtaacgccgg gaattgtttg
     1801 cgaaacagtt ccgccacgcc cggttgcata caagagccgc ccgccagcca taaatcagta
     1861 atgccctgcc cttcaatatg ccgggcgaca atgtccgcca ttttttcgta caccggtttc
     1921 accgtaggcc aaatctcttc accgtgaccg cgcttgtact gctccgcctc ttccagcgaa
     1981 atacggcgat ttccggcgag agtcagagag atgtgatgtc cgccggttgc ttcatccgcc
     2041 gagtacgtca ccttgccctt tttcacgatg gcaatgccgg tagtgccgcc gccgatatcc
     2101 actacaccgg cgttgtccag ttgcagcagc tccgcaactg cggttggctc atccagcaca
     2161 tggctcactt ccagtccagc agactccagc acgttgatgg aaatacgcgg gtccgtacct
     2221 ggaggaaatg aagtcgccgc atggctaaaa cggcgaccga attgctgctc gagcgtgtcg
     2281 agatggcgac gaacaatggt gacagcgccg aagaaatccc agacgatgcc gtcgcggacc
     2341 acatcggccc agtcgaggca taccgccacc ggctgaccgt cgcggtcgac aaccatcgac
     2401 accacatcgc aggtgcccag atccaccccc agccacagcg gagattccgt cgcggcgggc
     2461 gtctggttac acagcgttgc cgccgtttgc agacgtgggg tgagccattg ttcgtcgtgc
     2521 gccatctgtt actccttaaa caatgcgaaa cgcatcgact aatacacagc gacgcagacg
     2581 gacaaacgta cgcgcgctgg ttaccccttc accggttggc gtggtgatgg tcatggtggt
     2641 ccagccttcc ccgcccagcc ccagcccggc aatgcacggt ccgttcttaa cgaaaatgct
     2701 ggtatcaata gcattcgcca tctggttcat gttttcgatg ttgcgcgagt gcattgccgc
     2761 cgtgtggtgg caaccgcctt ccagtttcac cgccagcgca atggcatccg ccacgttggc
     2821 gacgcgcacg acgggcagca ccggcatcat cagttcggtc acggcaaacg gatgttctgc
     2881 agtggtttcc acaaacagca aacgcgtttc ttgcggcacg ttcaggccga ttgccgccgc
     2941 gattttcgcc gcatcgcgac caacccagtc acggctgacg gtgccttttc cgcgctcgtc
     3001 gatatttttc agcaacaccg gttgcagctg ctgcgcctgt tctgcggtca gtttcaccgc
     3061 gtgctggcct tccatcagac gcatcagttc atcggctacg ctatcaacaa caatcagtac
     3121 cttttcgtcg gcacaaatga tgttgttatc gaaagaagcg cctttgacga tggactgagc
     3181 ggcacgggcg aggtcggcgg tttcatccac cactaccggc gggttgccag cgcctgcggc
     3241 aatcagacgt ttattggtgt gtttacgcgc cgcttctact accgcttcgc cgccggtcac
     3301 caccagaagg ccgatgcccg gaaacttgaa caaacgctgc gcggtttcga tatccggatt
     3361 tgccacagta accagtaagt tttccggccc gcctgcggca acaatcgcct ggttgagcag
     3421 cgtgatcgcc cgctgggaga cttttttcgc cgccggatgc ggggcaaaaa tgacgctgtt
     3481 gcccgcggca atcaggctga tggcgttatt aattacggtt gccgccgggt tagtggacgg
     3541 agttaccgaa gccaccacgc cccagggcgc gttttcaatt aaagtcaggc cgttgtcgcc
     3601 ggtcagcacc tgcggagaga gacactcaac gcctggcgtg ccgcgcgcct gagcgacgtt
     3661 tttggcaaat ttatcttcaa cgcggcccat gccggtttca ctgacggcaa gttccgctaa
     3721 atctctggcg tgtttttcgc ctgcctcacg aatggcagca atggctaact ggcgcattgc
     3781 cacgcttttt aacccttgct gggcgacttt ggctgccgca acggcgtcat ccagggacgc
     3841 gaaaacgccc atctcatgaa cggcggcggg cggcgtgtca ctgctttgca ttttcagcag
     3901 taccgctttc accacctgtt caatatcctg ttgattcatg atgttctgcc ttatttgtgg
     3961 aaaattacct gaccgccaga caccacctca tcgacaatgc caatcacgca cagatcgacc
     4021 ggtgacgttt cgcttttatg cgcctggcgg gcggaactgc cactcaccag taacacccac
     4081 tccccggttc ccgcgccaat attgtcgatg gcgacggcgc attgcccgtc aggattacct
     4141 tgtggatcaa tcatttccac catcagcaat ttgtcatgcg ccagtccgtg atggcgtacg
     4201 gtacaaacaa tttgtccagt gacgactgcc agtttcatac ccgcctccgt ggcgtatttc
     4261 aggtaaaagc tccccctacc ctccgcagaa ggtaaaatga aaaaggagag agcgtaacgc
     4321 ccgaatcgac gtcacacagg gtgattacag gttgctgcta tcgcctttca ggccgatcgg
     4381 gaagacttct tccaggtcgc cgtgcggacg cgggataacg tgtacagata ccagctcgcc
     4441 gatacgctgt gccgctgcgg cacctgcatc ggtcgcggct ttacaggctg caacatcacc
     4501 acgaaccatg gcagtacaga ggccgccgcc aatctgctta acaccaacca gcttgacgcg
     4561 cgcagctttc accatcgcgt cagaagcctc aatcagtgca accaggcccc gggtttcgat
     4621 cattcctaat gcttccattg tgctttcctc tttatcaggg tccagaacgg gaccgttcat
     4681 tcaaccagtg tttgtaaact gctttcgcgg ttcacttctg tctgacgcgg cacagctgcc
     4741 accagcgcca gctcgataat ttcctgcacg ctacaaccac gagagagatc gtgcatcggc
     4801 acggcaagtc cttgtatcag tggcccgacg gcacgatatc cgccgagtcg ttgtgcgatg
     4861 ttgtaaccaa tatttccggc ttccagcgac ggaaaaacca tcacattggc cttgccctgt
     4921 agcgggctgg caggcgcttt ttgcgccgcc acttccggca cgaaggcggc gtcaaactgt
     4981 aactcgccat ccaccaccag ctttggtgcg cgctcacgga cgatttctgt cgcctgctgg
     5041 acgttagcaa cacaggggtg acgggcgcta ccattgctgg aaaacgacag catcgccacg
     5101 cgcggctctt ctccggtgat ggcgcgccag gtttcggcac tggcaagcgc gatatccgcc
     5161 agctgcgccg ccgtcggctg tggcaccacg ctgcaatcgg caaagcccaa cgccgggccg
     5221 ctgtactgtg gcaacatcag gaaaatgaaa gagagcgttt tacagcctgg ctgcaagccg
     5281 ataatgcgta atccggcacg cagcacattt gccgtggaag agaggttgcc tgcgatacag
     5341 acatccgctt taccggcgct gaccattgct gcggcgaaca tcagagggtc ggtaagtttt
     5401 tccagcgcat ccggcggcgt tttttcgccc gcgcgggcca gccagcgatg agcaaattct
     5461 tcccgcattg cgaggttgcc atgcggatca atcacctgta gcccgtccat cgccacgccg
     5521 tgactgagcg caaactgacg aagttcaaac ggattggcga ccagaatggg cgttgccaga
     5581 ccttgttgat gtaaatattg cgcggctttc agcacacgtt gatctaacgc atccggaaaa
     5641 accactctgg cgggcgctcg caacgccagt tcacgacaac gttcaataat cattgcgttc
     5701 ccccagtcgt tgttggatct gcttcaccgt tagcggctgc ttcgttacgc tcaggatcat
     5761 catcacgtag accgtgctgg aaaggcggtt cagcgcctgc aaaatgtccg ggcgtaatac
     5821 ttcaaagctg cgggtgataa agacctgcgc cgctacggtt tcggtttccc gcactttggt
     5881 gcgtagcaga ttgagcaacg cagcatcgcg cccgtggctg gcttccagca ccagatgatc
     5941 gtggtcgaga tagcgcagcg gctgatgaga aagccgatgc agatcttcgt cagaaagccc
     6001 gacaatcgcc tggcagccca gcggttcccc cagcgcatcg gcgcgcataa tgttgccaag
     6061 acgcgaacgg atatccgcca gccacggctg ccacggttcc gccagttcaa tttgcagcca
     6121 cactgccagc gcaatggtgc tgtcgagaac cgcgcgaaaa cccagacgcg gatcgctttt
     6181 ggcgaccatt ttttccgccg acaggtgagt cagcgtatcc ggcttttttg ccaccggctg
     6241 gcggcacagt tcgcagcacg cctgcggatg ttcatcgcta ctggtcagcc catgaacggg
     6301 ctgcggcttc tgctgttcat cgtcaacaaa caggcggccc tgctcgtcaa taaacttgat
     6361 gcgcagatgg cggctttcca gcaactcccg ggcagagggc gtcaggcgac tgtccgcagg
     6421 gagatggatc tctgcgcctt cgctgagcgt atggttcgct cttagccatg cttcggtgat
     6481 gaaatctttc atagggattg ccagttagcc ggccaggcga catacaggaa tttcacgctg
     6541 gatgtcatac caaattcgat gctggagcct ttcgggataa acatcacgtc gcctgctttg
     6601 gcgatcatgg tttggccttc gtggcgaaca tgcagcaccc cttccagcac catgtcgatt
     6661 tcgtcgtagt tcagggtcca cgggaagaat gcgttctccc actgcataaa accggcggcc
     6721 atgctgctgc cgtcgtctcc ggtcacaaga tcagtcaagc ccacgcaatg cggctctgcg
     6781 ccgtcgaaac gaccaaactt gacgctgctg ccatcaatca ctttgatgcc gcctttgccg
     6841 gtcaccgatt tgaagctcgg ctgcattgcg ccctgctcca gcgactgttt ttccttcatc
     6901 actttttcca tcagctgtgc gaccaggctt tcggtaaact ggccttccgg cagctgggca
     6961 atgatggttt cgcggatgcg ctggctttcg gttttgtcgg ctggcacgct ggcgggcacg
     7021 gacgccgtta ccgggatcga ttcatcacat tcggtgatgg taaaacccag tagatccgcc
     7081 acttcacggg cttccggggt aataatgctg gcgcgcagaa ccacggacat tgcctgttcg
     7141 ccgcgcgcgt gtgcttcacg aatatcattc gctgtgatga gttttttcac ctgcttcctc
     7201 ctgtttggtg agagatgcca gataatccac cagttgctgc acgctttgcg ggtcgtggct
     7261 gtttagctcg aaaatcggct cttcgaaacc cgtctcaagc aataactttc gcgttgcggc
     7321 gacgtcagca tctggcatgt ccgttttact gatgacggcg atttgccgtt tgctgacgcc
     7381 aatatccagc aacccggcag gcaaccgact ttccgggtca tttgcgccat ggacatagat
     7441 cagcatgtct acatcctgta gcgtggtaat taaggcgtga taccagcggg gatggctaaa
     7501 atattcaccc ggcgtatcaa tatcgccctt atcattaaat tccacggcct gtgtttttct
     7561 ggcgagggta taattcccct gcaacgcatt aaataacgtt gtttttcctg ccccgacact
     7621 acccacaaac gcaattcgtt tcatgaatgc ctcgtattaa cttttggtca tttcacaaag
     7681 cgtataattt aataagcgac ctaaaccgct gacggtttgt gataacgctt cttctactgc
     7741 gcccaccgag ccataaatca ccaatgcacc gctgaagcgg tcgagaaaac cgatatgcac
     7801 atcagcggct ttcagcgcta aatcaccggc gatcatcgcc gtttcgccgg gagttagcgt
     7861 cataatgcca attgcgcccg catccggaac gccgattttc ttcgccagtt cctcaccggg
     7921 atgcgagatg agatgcgcca gtgtgacctg tttgccgggc acaaattcct gaatgatgcg
     7981 ttctttatcc atgagtcgcc gcctgtgaaa aactttcgtg tatcgtgaca atttttcgtc
     8041 agagtaaata acaaaattcg gcaagcgcgt ttaaaaggtg aggtagatca ctaagaatgg
     8101 agagatattc gctgtggtgc ataaactttc atatgcaacg catgaatata aataaaataa
     8161 tatcgagata ttatatagac tgctttttta ttattcgcaa actcagtaat aagctttact
     8221 aatatattgc agccaaaccg cttaccctga atattcaggg taagcgtgag agttaaaaaa
     8281 gaattacagc ggttgggttt gcgcttcaac cacggccagc gccaccatgt tgacgatacg
     8341 acgcaccgat gcgatcggcg ttaacacgtg aaccggtttc gccacgccca tcagcaccgg
     8401 accgacagtc acaccttccg agctggaaac gcgcagtaag ttgtaactaa tgcgggcagc
     8461 ttccatgttc ggcatcacca gaatattggc agaacctttc aaagggctgt ccggcatacg
     8521 gtcgttgcga atcgcttcca ccagcgctgc atcgccgtgc atttcaccat caatcatcag
     8581 atctggcgca cgatccctga ccagttccag cgcctgacgc attttgctcg acgacgggca
     8641 gtcagaagaa ccaaagttgg agtgcgacaa cagagcaacg cgcggctcaa taccaaaacg
     8701 acggacagtt tctgccgcca tcaaggtgat ctccgccagc tcttctgcgt ctggttcgtc
     8761 attgacgtag gtatcggcaa taaaggtgtt accactcggc agcagcagcg cgttcattgc
     8821 acctgcggtg tgaacgccat cgcgataacc aaagacattt ttcaccacgc taaaatgttc
     8881 atgataatcg cccaccgtac cgcagatcat tgcatcggct tccccacgct gaaccatgat
     8941 cgcgccgatc actgtcgggt tactgatcag cgcccgctgc gcctgttcct gagtgacacc
     9001 gcgacgcttc atgatctgga agtattcggt ccagtactct ttaaagcgcg gatcggattc
     9061 gttattgacg atctcaaaat caacgcccgc tttgatctgc aagcccagtt tctgaatgcg
     9121 catttcgatc acgttcggac gaccgataag gatcggtttc gccagtccca gcgttaccag
     9181 ttcctgagtg gcatgcagaa cgcgcgcctc ttccccttct ggcagaacaa cgcgcttcgg
     9241 cgctttgcga gcctgggaga aaatcggctt cataaacagg ttggttttgt aaacgaactc
     9301 agtcagcttg tcgatgtaga cgtcgaaatc agcaatcgga cgcgtcgcca cgcccgactc
     9361 catcgcggct ttagcgaccg caggagcgat cttaacgatc aagcgcggat caaacggttt
     9421 tggaatgatg tattccggac caaagctcag atcctgatcg ccatacgctg aagccaccac
     9481 ttcgctctgt tccgcatggg cgagttctgc aatcgcacgt accgccgcca gtttcatctc
     9541 ttcgttgatg gcggttgcgc caacgtccag cgcgccacgg aagatgaacg ggaagcacag
     9601 gacgttgttc acctggttcg gatagtcaga acgaccggtg caaatgatgg catccggacg
     9661 cacttctttc gccagcggcg gcagaatttc cggttccggg ttcgccagcg ccaggatcat
     9721 tggcgcacga gccattttct tcaccatttc ctgggtcagc actttcgggc cggaacatcc
     9781 gaggaaaata tccgcgcctt caatcacatc atcgagggta cgtttgccgt cgtccaccac
     9841 cgcatatgcg gctttggttt ccgccatgtt tggctcacgg ccctgataga taacgccttt
     9901 tgaatcgcaa accacgatgt tatgtttttg cagacccagc gctaccagca ggttcataca
     9961 ggcgattgct gcggcacccg cgccggaaac caccatccgc acgtcggaga tgtttttctc
    10021 caccacgcgc aagccgttga ggatggcggc agtgctgatg attgccgtgc cgtgctgatc
    10081 gtcgtggaat accggaatat tcatccgctc gcgcagtttc tgttcaatat agaaacattc
    10141 tggcgcttta atgtcttcga ggttgattcc gccgaaggtc ggttcgagcg cagcgacaac
    10201 ttcaataaat ttgtccgggt cgagttcatc aacttcaatg tcaaatacat caatcccggc
    10261 gaatttctta aacagaacgc ccttgccttc catcaccggt ttgccagcca gcgcgccaat
    10321 gttgcctaac cccagcaccg ccgtaccgtt agagatcacc gccaccaggt taccgcgcgc
    10381 ggtatatttg taggctttta acgggtcttt ttcgatttca agacaaggtg cggcaacgcc
    10441 tggtgagtag gccagcgcca gatcgcgctg cgttgccaga ggcttggttg gagaaacctg
    10501 gattttccct ggaactggaa attcatggaa atcaagtgca ctttgtttta actggtcatc
    10561 catttggttg ttcctttcac gtaacgttca caaataaagt gtgtgggcaa cagcccctgc
    10621 ccacaacgtg gcgcacatta ttaccccgcc ggagtctaca gactttgagc aagtccaaac
    10681 tctcaccatt aatataatgt tttggtaata atcctataac actgatgtta cctgcttaat
    10741 ccagcaatac catgcctgtc tgctatgctt ttttgatgcg tttagcgaaa tttctcagaa
    10801 gtgtgaatta acgcactcat ctaacacttt acttttcaag gagtatttcc tatgaacgag
    10861 ttagacggca tcaaacagtt caccactgtc gtggcagaca gcggcgatat tgagtccatt
    10921 cgccattatc atccccagga tgccaccacc aatccttcgc tgttgctcaa ggctgccgga
    10981 ttatcacaat atgagcattt aatagacgat gctatcgcct ggggtaaaaa aaatggcaag
    11041 acccaggaac aacaggtggt cgcagcgtgt gacaaactgg cggtcaattt cggtgctgaa
    11101 attctcaaaa tcgtacccgg tcgcgtgtca acagaagttg atgcacgcct ctcttttgat
    11161 aaagaaaaga gtattgagaa ggcgcgccat ctggtggact tgtatcagca acaaggcgtt
    11221 gagaaatcac gcattctgat caaactggct tcgacctggg aaggaattag cgcggcagaa
    11281 gagctggaaa aagaaggtat taactgcaac ctgacgctgc tgttttcttt tgcccaggcg
    11341 cgggcctgtg cggaagcagg cgtttttctg atttcgccgt ttgtcgggcg tatttatgac
    11401 tggtatcagg cacgcaagcc gatggccccg tatgtggtgg aagaagatcc gggcgttaaa
    11461 tcggtgcgca atatctacga ctactataag caacaccact atgaaaccat tgtgatgggc
    11521 gcgagcttcc gtcgcaccga acaaatcctc gccttaaccg gctgcgatcg actgactatc
    11581 gcaccgaatt tgctgaagga gctgcaggaa aaagtttcgc cagtggtacg taaattaatc
    11641 ccaccttctc agacgttccc acgcccagct cccatgagcg aagcggagtt ccgttgggag
    11701 cacaatcagg atgcgatggc ggtagaaaaa ctgtctgaag gcattcgtct gttcgccgtt
    11761 gatcaacgca aactggaaga tcttcttgcc gccaaactat aaaccagcca cggagtgtta
    11821 tatgtcccga aaagaccttg ccaatgcgat tcgcgcactc agtatggatg cggtacaaaa
    11881 agccaactct ggtcatcccg gcgcgccgat gggcatggct gatattgccg aagtgctgtg
    11941 gaacgatttt cttaaacata accctaccga cccaaccggg tatgatcgcg accgctttat
    12001 tctttccaac ggtcacgcgt cgatgctgct ctacagtttg ctgcatctga ccggttacga
    12061 cctgccgctg gaagagctga agaacttccg tcagttgcat tcgaaaaccc caggccaccc
    12121 ggagattggt tatacgcccg gtgttgaaac caccaccggt ccacttggac aaggtttggc
    12181 gaacgccgtc ggactggcga tagcagagcg tacactggcg gcgcagttta atcagccgga
    12241 tcatgaaatt gtcgatcact tcacctatgt gtttatgggc gacggctgcc tgatggaagg
    12301 tatttcccac gaagtctgtt cgctggcggg cacgctagga ctgggcaagc tgattggttt
    12361 ttacgatcac aacggtattt cgattgatgg agaaaccgaa ggctggttta ccgacgatac
    12421 ggcaaaacgt tttgaagcct atcactggca tgtgatccat gaaatcgacg gtcacgatcc
    12481 gcaggcggtg aaggaagcga tccttgaagc gcaaagcgtg aaagataagc cgtcgctgat
    12541 tatctgccgt acggtgattg gctttggttc gccgaataaa gcaggtaagg aagaggcgca
    12601 cggcgcacca ctgggggaag aagaagtggc gctggcacgg caaaaactgg gctggcacca
    12661 tccgccattt gagatcccta aagagattta tcacgcctgg gatgcccgtg aaaaaggcga
    12721 aaaagcgcag cagagctgga atgagaagtt tgccgcctat aaaaaggctc atccgcaact
    12781 ggcagaagag tttacccgac ggatgagcgg tggtttaccg aaggactggg agaaaacgac
    12841 tcagaaatat atcaatgagt tacaggcaaa tccggcgaaa atcgctaccc gtaaggcttc
    12901 gcaaaatacg
//
LOCUS       DAJVUJ010000122        12738 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.122, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000122 DAJVUJ010000000
VERSION     DAJVUJ010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12738
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.122"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..471
                     /locus_tag="OQQ48_003062"
     CDS             <1..471
                     /locus_tag="OQQ48_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001589353.1"
                     /note="type IV secretion system coupling protein; similar
                     to F plasmid TraD; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HCS1953750.1"
                     /translation="YQERPKVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEP
                     DVPEVVSGEDVTQAEQPQQPVSPVINDKKSDSGVNVPAGGIEQELKMKPEEEMEQQLP
                     PGISESGEVVDMAAYEAWQQENHPDTWQQMQRREEVNINVHRERGEDVEPGDDF"
     gene            complement(480..878)
                     /gene="vapC"
                     /locus_tag="OQQ48_003063"
     CDS             complement(480..878)
                     /gene="vapC"
                     /locus_tag="OQQ48_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HCS1953751.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTPMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRTEDWS"
     gene            complement(878..1105)
                     /gene="vapB"
                     /locus_tag="OQQ48_003064"
     CDS             complement(878..1105)
                     /gene="vapB"
                     /locus_tag="OQQ48_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HCS1953752.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHSVSADFMDNREQPGMQERESF"
     gene            1187..1606
                     /locus_tag="OQQ48_003065"
     CDS             1187..1606
                     /locus_tag="OQQ48_003065"
                     /inference="COORDINATES: protein motif:HMM:NF020332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative relaxase"
                     /protein_id="HCS1953753.1"
                     /translation="MMSIAQVRSAGSAGNYYTNKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNRHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IEAHNQAVDFAVRHHPCGGTGLHTDDDGRTVRNGADR"
     gene            1593..6464
                     /gene="traI"
                     /locus_tag="OQQ48_003066"
     CDS             1593..6464
                     /gene="traI"
                     /locus_tag="OQQ48_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005079803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HCS1953754.1"
                     /translation="MLTGNLVMALFNHDTSRDQEPQLHTHAVVTNVTQHNGEWKTLSS
                     DKVGKTGFIENVYANQIAFGRLYREKLKEQVEALGYETEVVGKHGMWEMPGVPVEAFS
                     GRSQTIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAEWMQTLKETGFDIRAYRD
                     AAEQRAYTRTQTPGPASQDGPDVQQAVTQAIAGLSERKVQFMYTDLLARTVGILPPEN
                     GVIERARAGIDEAISREQLIPLDREKGLFTSGIHMLDELSVRALSRDIMKQNRVTVHP
                     EKSVPRTAGYSYAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIA
                     ADRRSQMNLKQDERLSGELITGRRQLLEGMAFTPGSTVIVDQGEKLSLKETLTLLDGA
                     ARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQRPATIISEPDRYVRYARL
                     AGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVLGHPEVTMTALSPVWLDS
                     RSRYLRDMYRPGMVMEQWNPETRSHDRYVTERVTAQSHSLTLRNAQGETQVVRISSLD
                     SSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASVSEDAMTVVVPGRAEPAT
                     LPVSDSPFTALKLENGWVETPGHSVSDSATVFASVTQMAMDNATLNGLARSGRDVRLY
                     SSLDETRTAEKLARHPSFTVVSEQIKARAGETLLETAISLQKAGLHTPAQQAIHLALP
                     VLESKNLAFSMVDLLTEAKSFAAEGTGFADLGGEINAQIKRGDLLYVDVAKGYGTGLL
                     VSRASYEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSGQRAAARMILETSDRFTV
                     VQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAVGEMRSAGVDAQTLASFL
                     HDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQA
                     IAPGQPFRLQQTRSAADVVIMKEIVRQTPELREAVYSLINRDVERALSGLERVKPSQV
                     PRLEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDVPMTLYAAIVRDYTGRTP
                     EAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGQVQVMVPVLNTANIRDGELRRLST
                     WENNPDALALVDNVYHRIAGISKDDGLITLQDAEGNTRLISPREAVAEGVTLYTPDTI
                     RVGTGDRIRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQTRVIRPGQERAEQHID
                     LAYAITAHGAQGASETFAIALEGTEGNRKLMAGFESAYVALSRMKQHVQVYTDNRQGW
                     TDAINNAVQKGTAHDVFEPKPDREVMNAERLFSTARELRDVAAGRAVLRQAGLAGGDS
                     PARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGNGLRGFSGEGRVKGSGDA
                     QFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGEGRPWNPGAITGGRVWGD
                     IPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLPDGKTE
                     QAVREIAGQERDRAAITEREAALPESVLREPQRVREAVREVARENLLQERLQQMERDM
                     VRDLQKEKTPGGD"
     gene            6484..7230
                     /gene="traX"
                     /locus_tag="OQQ48_003067"
     CDS             6484..7230
                     /gene="traX"
                     /locus_tag="OQQ48_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HCS1953755.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSNRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVLC
                     AGKSLPRFWPGDFFPTFYACHLAVLGVLAL"
     gene            7285..7845
                     /locus_tag="OQQ48_003068"
     CDS             7285..7845
                     /locus_tag="OQQ48_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HCS1953756.1"
                     /translation="MTEQKRPVLTLKRKTEGTATVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAGARLAKIRRQNRIKAELQAVLDEK"
     gene            7976..8188
                     /locus_tag="OQQ48_003069"
     CDS             7976..8188
                     /locus_tag="OQQ48_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HCS1953757.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRG"
     gene            8432..8893
                     /locus_tag="OQQ48_003070"
     CDS             8432..8893
                     /locus_tag="OQQ48_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCS1953758.1"
                     /translation="MRKYIPLVLFIFSWPVLSADIHGRVVRVLDGDTIEVMDSLKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVTYFQRDRYGRILGQVYAPDGMNIN
                     QFMVRAGAAWVYEQYNTDPVLPVLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            8939..9148
                     /gene="hha"
                     /locus_tag="OQQ48_003071"
     CDS             8939..9148
                     /gene="hha"
                     /locus_tag="OQQ48_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCS1953759.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            9187..9777
                     /locus_tag="OQQ48_003072"
     CDS             9187..9777
                     /locus_tag="OQQ48_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HCS1953760.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSSGEYWQRQTTLLTEREVSFMKGLFRIVDMTRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVELRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            10017..10277
                     /gene="repA"
                     /locus_tag="OQQ48_003073"
     CDS             10017..10277
                     /gene="repA"
                     /locus_tag="OQQ48_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HCS1953761.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            10501..10575
                     /gene="tap"
                     /locus_tag="OQQ48_003074"
     CDS             10501..10575
                     /gene="tap"
                     /locus_tag="OQQ48_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HCS1953762.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            10568..11425
                     /gene="repA"
                     /locus_tag="OQQ48_003075"
     CDS             10568..11425
                     /gene="repA"
                     /locus_tag="OQQ48_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HCS1953763.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCGKLMEKAVGFTSR
                     FDFAIHVAHARSLGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLAELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAVAAARRSRVEWENRQRKKQGLDTLGMDELMAKAWRFVRERFRSYQTELKSRGMK
                     RARARRDADRQRQDIVTLVKRQLTREISEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            complement(12477..>12738)
                     /locus_tag="OQQ48_003076"
     CDS             complement(12477..>12738)
                     /locus_tag="OQQ48_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCS1953764.1"
                     /translation="QAAAFLGVVPVEKYSGTSVRGCPKLSKIGPPEIRAKLYLASLCG
                     LRFNPLMKAMYERLCLRGKAKMCAIGALMRKLVHWCYGVLKT"
BASE COUNT         3087 a         3211 c         3781 g         2659 t
ORIGIN      
        1 tatcaggaac gaccgaaggt cgctccggag tttattccgc gtgacatcaa cccggaaatg
       61 gagaaccgtc tgagtgccgt acttgccgca agggaagcag aaggtcgtca gatggccagc
      121 ctcttcgaac cggatgtccc ggaggttgtt tccggagaag acgtgactca ggctgaacag
      181 ccgcaacagc cggtgtctcc tgtcatcaac gataagaagt cagattcagg tgtgaatgtt
      241 ccggcagggg ggatcgagca ggagctgaaa atgaaaccgg aagaagagat ggaacagcaa
      301 ctgccacccg ggatcagtga atccggtgaa gtggtggata tggccgctta tgaggcatgg
      361 cagcaggaaa atcatccgga cacctggcag cagatgcagc gtcgtgaaga ggtgaacatc
      421 aatgtgcacc gggaacgcgg ggaggatgtt gagccgggag atgatttctg atgaacaggt
      481 cagctccagt cttcagttct caggccgccc acacgttcaa attcccgggt gttattagtc
      541 acaataatca gcccccgact gcgggcatga cctgcgatca tttgatcaaa tggcccgaca
      601 gggcgtccct gacgggcaag ttctgctctt atctggccgg tgtgtgtggc tgcagcagcg
      661 tcgtaatcca gaacatcaat gcgggaaaca aatccctcga tcacagcgag attacgttca
      721 ggcatctggc ttttttctgc accatatatc agctccatcg gggtgaccga actgatacac
      781 attctcccct ggttcaggtt aaaacgctcc ctgacgctgg cgggtttgtt ctttatcgta
      841 aaaatgcaga tgttggtatc gagcataaac ttcagcatca gaatgactcc ctttcctgca
      901 tgccgggctg ttccctgtta tccataaaat cggcgctgac gctgtgtccg tcgaaccatt
      961 cgtcccatgt ctctccggct ggcgtaatga ttctggttcg tccgacagca atcacttcaa
     1021 cgcgttttac gttttccggc aatgcaaccg cttttggcag tctgaccgcc tggctgcggt
     1081 tgctgagaaa tacggtggtt tccattattt gcctccttat gtggatatgt cttgtgtata
     1141 tccattgtgg gattgatgcc gggatatgtc aaagggatat acgtttatga tgagtatcgc
     1201 gcaggtcaga tcggccggaa gtgccggtaa ttattatacc aacaaggata attactatgt
     1261 gctgggcagc atgggagaac gctgggccgg ccagggggct gaacaactgg ggctgcaggg
     1321 cagtgtcgat aaggatgtct ttacccgcct tctggagggc aggctgccgg acggggcgga
     1381 tttaagccgc atgcaggatg gcagtaacag gcatcgtccc ggctacgacc tgaccttctc
     1441 cgctcccaaa agtgtctcca tgatggccat gctcgggggg gacaaacgtc ttattgaagc
     1501 acataaccag gccgtggatt ttgctgtccg tcatcacccg tgtggaggca ctggcctcca
     1561 cacggatgat gacggacgga cagtcagaaa cggtgctgac cggtaatctg gtgatggcac
     1621 tgtttaacca cgacaccagt cgcgatcagg aaccacagtt acacacgcat gcggtggtga
     1681 ctaatgttac gcagcataac ggcgaatgga agacactgag cagtgacaaa gtggggaaaa
     1741 cggggttcat tgagaatgtg tacgctaatc agattgcctt tggcaggctc taccgggaaa
     1801 aactgaaaga gcaggttgag gcgctgggct atgaaactga agtggtcggt aagcacggta
     1861 tgtgggaaat gccgggtgta ccggtggagg ccttttccgg acgctcacag actatccggg
     1921 aggccgtcgg ggaagacgcc tcgctgaaat cccgggatgt ggcggcgctg gatacgcgta
     1981 aatccaaaca gcacgtcgat cctgaagtca gaatggccga gtggatgcag acgctgaagg
     2041 aaaccgggtt cgacatccgg gcatatcgtg acgcagcgga acaacgggca tatacccgca
     2101 cgcagacacc cggacccgct tcacaggacg ggccggatgt gcagcaggcg gtgacacagg
     2161 ccattgccgg attaagtgaa cgcaaagtgc agtttatgta tacggacctg ctggccagga
     2221 cggtcggcat actgccgccg gaaaatggtg tgattgaacg ggcacgcgcc ggtatcgatg
     2281 aggccatcag ccgtgagcag cttatccccc tcgatcgtga gaaggggctg ttcacgtccg
     2341 ggattcatat gctcgatgag ctgtcagtcc gggcactcag tcgtgacatc atgaaacaga
     2401 accgggtgac cgtacatccg gagaaaagtg tcccccggac ggccggttac agctatgccg
     2461 tcagtgttct ggcacaggac cgtccgtcgc tggccattgt gtccgggcag ggcggtgcag
     2521 ccgggcagcg tgagcgggtg gctgaactgg tcatgatggc ccgggagcag gggcgggagg
     2581 tgcagattat cgctgctgac cgtcgctcgc agatgaacct gaagcaggat gaacggctgt
     2641 ccggtgaact gataaccgga cgtcgtcagt tgctggaagg catggccttc acgccgggca
     2701 gtaccgttat cgtcgaccag ggcgaaaaac tctccctgaa agagacgtta accctgctgg
     2761 atggtgccgc acgtcataac gtacaggtcc tgataactga cagcgggcag cgaaccggta
     2821 caggcagtgc actgatggcc atgaaggatg ccggggtgaa cacatatcgc tggcaggggg
     2881 gagaacagcg accggccacc atcatcagtg aaccggaccg ttatgtccgc tatgcccggc
     2941 tggccggaga ttttgcggcc agcgtgaaag ccggagaaga gagcgtggca caggtcagcg
     3001 gagtacggga acaggccata ctgacacagg ccattcgcag tgagctgaaa acacagggcg
     3061 tgctcggaca cccggaggta accatgaccg ccctgtcacc ggtctggctg gacagccgga
     3121 gccgttatct gcgggatatg taccgcccgg gaatggtgat ggagcagtgg aacccggaga
     3181 cacgcagtca tgaccgctat gtgacagaac gtgtgacggc gcagagtcac agcctgaccc
     3241 tgcggaatgc gcagggcgaa acgcaggtgg tgcgtatttc ttccctggac agcagctggt
     3301 cgctgttccg gccggaaaaa atgccggtgg cagacggtga gcgtctgagg gtgacaggga
     3361 aaattcccgg actccgcgtc tccggcggtg accgcctgca ggtggcatcc gtcagtgaag
     3421 atgcgatgac ggttgttgtg ccgggacggg ctgaaccggc caccctgcct gtgagcgatt
     3481 caccgttcac ggcactgaag ctggagaacg gctgggtgga aacgcccggg cattccgtca
     3541 gcgacagtgc gacggttttt gcctccgtca cacagatggc aatggacaat gccaccctga
     3601 acggtctggc ccgcagtggt cgtgatgtcc ggctgtattc ctcactggat gaaacccgta
     3661 ctgcggaaaa acttgcccgc catccttcct ttacggtggt ttctgagcag ataaaggcgc
     3721 gtgccggtga gacattgctg gaaaccgcta tcagtctgca gaaagccggg cttcacacgc
     3781 cggcacagca ggccattcat ctggcccttc ctgtgctgga aagtaaaaac ctggccttca
     3841 gcatggtgga cctgctgaca gaggcgaagt cgtttgctgc agaaggaacc ggttttgctg
     3901 acctgggagg ggaaatcaat gcgcagataa aacggggtga tttactgtat gtggatgtgg
     3961 caaaaggcta tggcacaggc ctgctggttt cccgtgcgtc gtatgaggca gaaaagagca
     4021 ttcttcgcca tattctcgaa ggtaaggagg cggtcacgcc gctgatggag agagtacctg
     4081 gcgaactcat ggagaaactg acatcaggac agcgcgccgc cgcccgtatg atactggaaa
     4141 cgtccgaccg tttcacggtg gtgcagggct atgccggtgt gggtaagacc acacagttcc
     4201 gggcggtgat gtcagccgtg aacatgctgc cggagagtga acgtccccgt gtcgtggggc
     4261 tggggcccac acaccgtgcg gtcggggaga tgcgcagcgc cggcgtggat gcacagacac
     4321 tggcgtcctt tctgcatgac acgcagctgc agcagcgcag cggagaaacg ccggatttca
     4381 gcaacacgct gttccttctc gatgagagct ctatggtggg caataccgac atggcacggg
     4441 catacgccct gattgcggcc ggtggcggtc gggctgtggc cagcggtgac acggaccagc
     4501 tgcaggccat cgcgcccggt cagcctttcc gtctccagca gacgcgcagt gctgccgatg
     4561 tggtcatcat gaaggagatt gtgcgtcaga cgccggaact gcgggaggcg gtatacagcc
     4621 tgattaaccg ggatgtggaa agggcgctgt ccgggcttga gcgtgtgaaa ccgtctcagg
     4681 tgccacgtct ggagggcgca tgggcaccgg agcactccgt gacggagttc agtcacagcc
     4741 aggaagcgaa actggcagaa gcgcagcaga aggcgatgct gaaaggcgag gcttttccgg
     4801 atgtccccat gacactgtat gcagccattg tccgcgacta taccggcagg acaccggaag
     4861 cacgggagca gacgctgatt gtcacgcacc tgaatgagga ccggcgcgta ctgaacagca
     4921 tgattcatga tgcacgggaa aaggccggag aactggggca ggtgcaggtc atggtgcctg
     4981 tcctgaacac agcgaatata cgcgacgggg agctgcgtcg tctctccacc tgggagaata
     5041 acccggatgc ccttgccctg gtggataatg tgtatcaccg gattgccggt atcagcaagg
     5101 atgacgggct gataaccctg caggatgcgg aaggtaacac gcggctgatt tcgccccggg
     5161 aggcggtggc tgaaggcgtc acgctgtaca ccccggacac catccgggtg gggaccggtg
     5221 acaggatacg cttcacgaag agtgaccggg agcgcggtta tgtggccaac agcgtctgga
     5281 cggtgacagc ggtttccggt gacagtgtca cactttctga cggtcagcag acccgtgtga
     5341 ttcgccccgg tcaggagcgg gcagagcaac atattgacct ggcctatgcc atcaccgccc
     5401 acggtgcaca gggggcgagt gaaacctttg ccatcgcgct tgaaggcacg gaaggtaacc
     5461 ggaaactgat ggccggcttt gagtcagcct acgtggccct gtcgcgtatg aagcagcatg
     5521 tgcaggtgta caccgataac cgtcagggct ggacggatgc catcaacaat gccgtacaga
     5581 aaggaactgc ccacgatgtg tttgagccga aaccggaccg ggaggtcatg aatgcagagc
     5641 ggctgttcag tacggcgcgg gagctgcggg acgtggcggc aggccgtgcc gttctccgtc
     5701 aggcggggct ggccggggga gacagtcctg cacggtttat tgctccggga cgtaaatatc
     5761 cgcaaccgta tgtggcactg ccggcgtttg accgtaacgg caagtccgcc ggtatctggc
     5821 tgaacccgct gaccacggat gacggaaacg ggctgcgggg attcagcggt gaaggtcggg
     5881 taaaaggcag cggggatgcg cagttcgtgg ctctgcaggg cagccgtaac ggagaaagcc
     5941 tgctggctga taatatgcag gatggcgtcc ggattgcccg tgataatcct gacagtggtg
     6001 tggtggtaag aatcgccggt gaaggccgtc cgtggaatcc cggtgccatc accggtggcc
     6061 gcgtgtgggg ggatatcccg gacaacagcg tccagccggg agccggaaat ggcgaaccgg
     6121 tcacggcaga ggtactggca cagcggcagg ctgaagaggc catccgccgt gaaacggaac
     6181 gtcgcgcaga tgaaattgtc cgtaaaatgg cagagaacaa acctgacctg ccggacggca
     6241 aaacagagca ggctgtcagg gaaattgccg ggcaggagcg tgaccgggct gccataactg
     6301 aacgggaagc cgcgctgccg gagagtgtac tgcgtgaacc acaacgggtg cgggaagcgg
     6361 tccgggaagt tgcccgggaa aatttgctgc aggagcgtct gcagcagatg gagcgggata
     6421 tggttcgtga cctgcagaaa gagaaaaccc cgggcggaga ctgatacagg aagacaaaca
     6481 ctgatgacaa cggataacac gaacacgaca cgtaacgatt cactggctgc acggaccgat
     6541 acctggttgc agtcatttct ggtctggtca cccggacagc gggatatcat caaaacggtg
     6601 gcactggtgc tgatggtact ggaccacatc aatctgatat tccagctgaa gcaggaatgg
     6661 atgtttctgg ccgggcgagg agcctttccg ctttttgccc tggtgtgggg gctgaatctg
     6721 tcccgtcatg cgcatatccg gcaaccggcc attaaccggc tgtggggatg gggaattatt
     6781 gcccagttcg catattatct ggcaggcttt ccctggtatg aggggaatat cctgtttgcc
     6841 tttgcagtgg cggcacaggt gctgacgtgg tgtgaaacgc gcagtgggtg gcgtacagca
     6901 gcggccatac tcctgatggc cctgtggggg cctttgtccg gcaccagtta cggtattgcc
     6961 gggttgctga tgctggcagt cagcaaccgg ctgtaccggg cggaagacag agcggaacgt
     7021 ctggcgctgg tcgcctgcct gctggctgtt attccggcgc ttaaccttgc caccagtgat
     7081 gcagcggcgg tggccggtct tgtgatgacg gtgctgaccg ttggtctggt gttgtgtgcc
     7141 ggaaaatcat taccccgttt ctggcccgga gactttttcc cgacgttcta tgcctgtcat
     7201 ctggctgtgc tgggcgttct ggcgctgtga cgggtgtggt atctttggcc gcaagaggat
     7261 gattcgtcag aggcagaaca cagcatgaca gagcagaagc gaccggtact gacactgaag
     7321 cggaagacag agggaacagc gactgtccgc agccggaaaa ccatcatcaa tgtcaccacg
     7381 ccaccaaagt ggaaggtgaa aaagcagaag ctggccgaga aagcggcccg tgaagcagag
     7441 ctggcggcaa aaaaagcgca ggccagacag gcgctgtcca tttatctgaa cctgcccacg
     7501 ctggatgagg ctgtgaatac cctgaagccc tggtggccgg gattatttga cggtgacacg
     7561 ccccggcttc tggcctgcgg tatccgggac gtgttactgg aagacgtggc gcagcggaat
     7621 atcccgctct cgcataaaaa actgcgcagg gcgctgaagg ccatcacccg ttcagaaagc
     7681 tatctgtgtg ccatgaaagc cggtgcctgc cggtatgaca cggaagggta tgtgacggag
     7741 catatttctc aggaggagga agcgtatgcg ggagcgcgtc tggcgaaaat tcgtcgccag
     7801 aaccggataa aggcagaact tcaggccgtg cttgatgaga aataaaaaaa gcctccccca
     7861 gagagaggag gcagggaaat aaggcttaaa aggaaataac ttccgcttcc gaaaggaagc
     7921 cagtccactg aattatttat cacaataata ctgacaacta aagggggaca agaaaatgaa
     7981 tggattcaga aacagttcac ggaacggcca ggtctggcgt taccagcgtg ccggcgggcg
     8041 ggccgttatt ctggaagtca gtggacgctg gatggaagcg gcagaagcat ggcgacgggc
     8101 tgcctgtgtt gcgcctagga cagactggca gcagtttgcc cggaaaaggg ctgaacactg
     8161 ccaccggcgc tgccggggaa gggggtaacg aaaaaagcag ccttcattac tccaggagtc
     8221 agaaggctga aaggagttta tgagatttgc ttcttaatat ccggtcgggc agtatgaccg
     8281 gacagttctg agtagcacag agtcttttcc gcattaagtc gattttttat attttgtgat
     8341 taatttcaca aaataaggtg ttattcaatg tgtgctgcac tattcagggg ggctgaaata
     8401 caggtgccat ttatctgata tggagaataa catgaggaaa tatattccac tggtattatt
     8461 tatcttttca tggccggtat tgtctgcaga tattcatgga cgggttgttc gtgtactgga
     8521 cggtgatacc atcgaggtca tggactcact gaaggcggtg cggatcaggc tggtaaatat
     8581 tgatgcaccg gaaaagaaac aggattatgg ccggtggtca acagacatga tgaaatcact
     8641 ggttgcagga aaaacggtta ccgtcacata ttttcagcga gatcgttatg gtcgtattct
     8701 cgggcaggtt tatgcaccag acggaatgaa tattaatcag tttatggtcc gggccggggc
     8761 tgcctgggta tatgagcagt acaacacaga tcccgtgcta cctgtccttc agaacgaagc
     8821 cagacaacaa aaacgtgggc tctggtcaga tgctgatccg gttcccccct ggatatggag
     8881 gcaccgtaaa taaagcgatg gcaatcctat cattcatctg ttctcagcgg gaataaaaat
     8941 ggcaaaaaca aaacaggaat ggctgtatca gttacggcga tgttcatcag tcaatacgct
     9001 ggaaaaaatt attcataaaa acagagacag tttatcaaac agtgaacgtg aatcatttaa
     9061 ttcagcagca gaccaccggc tagcggaatt aatcaccgga aagttatatg acaggatacc
     9121 aaaagagata tggaaatatg taaggtaata attcagcaat aattaattgc caggagcaga
     9181 taaataatga agttgattat ttttattctc attgttctga ttattgcagc gctacttatc
     9241 cggattatcc tcagaagtgt aaatcagcat tctcctttgc tgatgcaatt gcatgcggca
     9301 ggtatacgga ccggtgatgc agagcgaata ctgtccagcg gtgaatactg gcaacgtcag
     9361 acgacgctgc tgacagaaag ggaagtcagt tttatgaaag gactgttcag aattgtggat
     9421 atgacgcggt ggtatctgtg tccgcaggta cgggtcgcgg atatcgtcca gctgaacggg
     9481 aatatccggc cacgatcacg ccagtggtgg cagttattca ggatggtgtc tcagtggcat
     9541 gttgatgtgg ttatcgttga gctgcgttcg ttcagtattg ttgcagcagt agagctggat
     9601 gatgccagcc atttacgacc ggaacgcaga cgccgggata ttcttctgga agaggttctg
     9661 aggcaggctg gtattccgtt gctcagaagc cacgatgcca gaaaactgct gcagatgacc
     9721 ggagaatggc tgaatacaac aggggctgat cagcagtcac cggaacatcg tagctgacgc
     9781 cttcacgttg ctcagttgtc caaccccgga aacgggaaaa agcaagtttt cctcgctccc
     9841 ggcgtttcca taactgaaaa ccatactatt tcacagttta aatcacatta aacgacagta
     9901 atccccgttg atttgtgcgc caacacagat cttcgccaca attctcaagt cgctgatttc
     9961 aaaaaactgt agtatcctct gcgaaacgat ccctgtttga gtattgagga ggcgagatgt
    10021 cgcagacaga aaatgcagtg acttcctcat tgagtcaaaa gcggtttgtg cgcagaggta
    10081 agcctatgac tgactctgag aaacaaatgg ccgctgttgc aagaaaacgt cttacacaca
    10141 aagagataaa agtttttgtc aaaaatcctc tgaaagatct catggttgag tactgcgaga
    10201 gagaggggat aacacaggct cagttcgttg agaaaatcat caaagatgaa ctgcagagac
    10261 tggatatact aaagtaaaga ctttactttg tggcgtagca tgctagatta ctgatcgttt
    10321 aaggaatttt atggctggcc acgccataag gtggcaggga actggttctg atgtagatgt
    10381 acaggagcca gaaaagcaaa aaccccgata atcttcttta actttggcga gtcagaaaga
    10441 ttaccggggc ccacttaaac cgtatagcca acaatcaagc tatgcgggga gtatagttat
    10501 atgcccggaa aagttcaaga cttctttctg tgctcgctcc ttctgcgcat tgtaagtgca
    10561 ggatggtgtg actgatcttc aacaaactta ttaccgccag gtaaagaacc cgaatccggt
    10621 gttcacaccc cgtgaaggtg cccggacgct tccgttctgc gggaaactga tggaaaaggc
    10681 ggtgggcttc acctcccgtt ttgatttcgc cattcatgtg gcacatgccc gttcgcttgg
    10741 tctgcgtcgg cgcatgccac cggtactacg tcgccgggcc attgatgcgc tgctgcaggg
    10801 gctgtgtttt cactatgacc cactggccaa ccgcgtccag tgttccatca ccacgctggc
    10861 cattgagtgc ggactggcga cggagtctgc tgccggaaaa ctctccatca cccgggccac
    10921 ccgggccctg acgttccttg cagagctggg actgattacc taccagacgg aatatgaccc
    10981 gcttatcggg tgctacattc cgaccgatat cacgttcaca tctgcactgt ttgctgccct
    11041 cgatgtatca gaggaggcag tggccgccgc gcgccgcagt cgtgtggaat gggaaaacag
    11101 acagcgcaaa aagcaggggc tggataccct gggtatggat gaactgatgg cgaaagcctg
    11161 gcgttttgtt cgtgagcgtt tccgcagtta ccagacagag cttaagtccc gtggaatgaa
    11221 gcgtgcccgt gcgcgtcgtg atgcggacag gcagcgtcag gatattgtca ccctggtgaa
    11281 acggcaactg acgcgtgaaa tctcggaagg gcgcttcact gccagtcgtg aggcggtaaa
    11341 acgtgaagtg gagcgtcgtg tgaaggagcg catgattctg tcacgtaacc gtaattacag
    11401 ccggctggcc acagcttccc cctgaaagtg acctcctcag aataatccgg cacgcgccgg
    11461 aggcattcgc acgcctgaag cccgccagtg cacaaaaaat cagcgctaca tacaaaaaac
    11521 aacctcatca tccaccttct ggtgcatccg gtcccccctg tttttgatac aaaacacgcc
    11581 tcacagacgg ggaattttgc ttatccacat taaactgcaa gggacttcac cataaggtta
    11641 caaccgttca cgtcataaag cgccagccgt cagccctaca gggtgcaatg tatcttttaa
    11701 acacctgttt atatctcctt taaactactt aattacattc atttaaaaag aaaacctgtt
    11761 cactgcctgt ctgtggacag acagatatgc acctcccacc acaagcggcg ggccccgacc
    11821 gaagccggtt tagttacaac atgcaaacat aaacccctaa aaaacccaag tccgacgcag
    11881 aactgaaacc acaaagcccc tccctcataa ctgaaaagcg gccccgcccc ggcccgaagg
    11941 gccggaacag agtcgctttt aattatgaat gttgtaacta tattatcatc gctgtcagtc
    12001 ctctggctgg acgtatcgag tacacgctcg taagcggccc tcacggccct cacggcccgc
    12061 taacgcggag atacgccccg actgcgggta aacccttgtc gggctccgac cgcgcacaga
    12121 agctctgtca tagctgaaaa cgggtatggc ttggcagcta ccatgttctg tcaagaaaaa
    12181 tcgcttgaac tccgacgtac agccccctaa gtactgccag caagctgtca gcactaagtc
    12241 cccgatacag cgcggcatag ccgcgctaac ggggttaaaa gggcttacct atacttcatc
    12301 atcgctgaaa gcccgcatgg cctcgcaggg cagagatggg ataggtgaaa cctatcaata
    12361 cgtaccggct tacgccgggc ttcggcggtg ttactcctgt accatatgta actacatcat
    12421 gccgccttac atgcgctacg cgcatatgaa gacaaatagc ccacatcaaa cggtttttag
    12481 gtcttcagta cgccatagca ccagtgcacg agttttctca tcagcgcacc aatggcacac
    12541 attttggcct tacctctcag gcataatcgc tcatacatcg ctttcatcaa tgggttaaat
    12601 ctcagaccac acaacgaagc cagatacagt ttcgcccgga tttccggcgg accaattttt
    12661 gacagtttcg gacaccctct gaccgaggtg ccggaatatt tttcaacggg aacgacacct
    12721 aaaaatgcag ccgcctgt
//
LOCUS       DAJVUJ010000123        12723 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.123, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000123 DAJVUJ010000000
VERSION     DAJVUJ010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12723
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.123"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(642..794)
                     /locus_tag="OQQ48_003077"
     CDS             complement(642..794)
                     /locus_tag="OQQ48_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1953765.1"
                     /translation="MSDITAYRPEVVNENTDVICSTSIRRILAVRKSSLLQIDTIIRH
                     QVSDIS"
     gene            complement(879..1076)
                     /locus_tag="OQQ48_003078"
     CDS             complement(879..1076)
                     /locus_tag="OQQ48_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1953766.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNNEDKTDSAALLPC
                     IEQAREDIRTLRQLQLQHQNR"
     gene            complement(1088..1576)
                     /locus_tag="OQQ48_003079"
     CDS             complement(1088..1576)
                     /locus_tag="OQQ48_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1953767.1"
                     /translation="MKLSLMLEADRINVQALNMGRIIVDVDGVNLTELINKVAENGYS
                     LRVVEESDQQSTCTLPPFATLAGIRCSTAHITEKDNAWLYSLSHQTSDVGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVITLTRRYGVSLIHLDASAECLPGLPTF
                     NW"
     gene            complement(1573..1947)
                     /locus_tag="OQQ48_003080"
     CDS             complement(1573..1947)
                     /locus_tag="OQQ48_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCS1953768.1"
                     /translation="MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKHPEKR"
     gene            complement(2037..2405)
                     /locus_tag="OQQ48_003081"
     CDS             complement(2037..2405)
                     /locus_tag="OQQ48_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HCS1953769.1"
                     /translation="MSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCKAD
                     TLGSGGYVYLAVYPTPETKK"
     gene            complement(2568..2789)
                     /locus_tag="OQQ48_003082"
     CDS             complement(2568..2789)
                     /locus_tag="OQQ48_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HCS1953770.1"
                     /translation="MKIITRGEAMRIHRQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(2876..3352)
                     /locus_tag="OQQ48_003083"
     CDS             complement(2876..3352)
                     /locus_tag="OQQ48_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HCS1953771.1"
                     /translation="MQQLSFLPGEMTPGERSLIQRALKTLGRHLHEPGVAFTSTHAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALALVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            complement(3368..3853)
                     /locus_tag="OQQ48_003084"
     CDS             complement(3368..3853)
                     /locus_tag="OQQ48_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HCS1953772.1"
                     /translation="MKTISHNSTTSSVSVTAASGNDQPQLVATLVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            complement(3908..4729)
                     /locus_tag="OQQ48_003085"
     CDS             complement(3908..4729)
                     /locus_tag="OQQ48_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HCS1953773.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELMHHVPGIFGEDKHTSRSQN
                     YTYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEII
                     LLNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVF
                     DRIEEKRDAMQSLILPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESLR"
     gene            4810..5021
                     /locus_tag="OQQ48_003086"
                     /pseudo
     CDS             4810..5021
                     /locus_tag="OQQ48_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386164.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5142..5597)
                     /locus_tag="OQQ48_003087"
     CDS             complement(5142..5597)
                     /locus_tag="OQQ48_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1953774.1"
                     /translation="MIHLFKTCMITAFILGLTWSAPLRAQDQRYISIRNTDTIWLPGN
                     ICAYQFRLDNGGNDEGFGPLTITLQLKDKYGQTLVTRKMETEAFGDSNATRTTDAFLE
                     TECVENVATTEIIKATEESNGHHVSLPLSVFDPQDYHPLLITVSGKNVN"
     gene            complement(5673..8189)
                     /locus_tag="OQQ48_003088"
     CDS             complement(5673..8189)
                     /locus_tag="OQQ48_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1953775.1"
                     /translation="MLQIVGALILLIAGFAILRLLFRALTSTASALAGFILLCLFGPA
                     LLAGYITERITRLFHIRWLAGVFLTIAGMIISFMWGLDGKHIALEAHTFDSVKFILTT
                     ALAAGLLVLPVQIRTIQQSGLTPEDISKEINGYYCCFYTAFFLMACSAYAPLIALQFD
                     ISPSLMWWGGLLYWLAALVTLLWAASQIQALKRLTSAISQTLEEQPVLNSKSWLSSLQ
                     NDYSLPETLTERIWLTLISQRISRGELREFELADGNWLLDNAWYERNMAGFNEQLKEN
                     LSFTPDELKTLFRNRLNLSPEANDDFLDRCLDGGDWYPFSEGRRFVSFHHVDELRICA
                     SCGLTEVHHAPENHKPDPEWYCSSLCRETETLCQDISERSYTGFISDATANGLILMKL
                     PETWSTNEKMFASGGQGHGFAAERGNHIVDRVRLKNARILGDNNARNGADRLVSGTEI
                     QTKYCSTAARSVGAAFDGQNGQYRYMGNHGPMQLEVPRDQYAGAVETMKNKIREGKVP
                     GVTDPAEASRLIRRGHLTYTQARNITRFGTIESVTYDIAEGSVISLAAGGISFALTAS
                     VFWLSTGDRDAALQTAAVQAGKTFTRTLAVYVTTQQLHRLSVVQGMLKHIDFSTASPT
                     VRLALQKGTGAGNISALNKVMKGTLVTSLALVAVTTGPDMIKMLRGRISGAQFIRNLA
                     VASSGVAGGAVGSVAGGILFSPLGPFGALTGRVVGGVLGGMIASAVSGKIAGALVEED
                     RVKILAMIQEQVTWLAGSFLLTGHEIENLNENLARVIDQNALEIIFAAGIQQRAATNM
                     LIKPLVVSIIRQRPVMEYDASHLGNMVNRLEEAFPPELPA"
     gene            complement(8310..11429)
                     /gene="ag43"
                     /locus_tag="OQQ48_003089"
     CDS             complement(8310..11429)
                     /gene="ag43"
                     /locus_tag="OQQ48_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HCS1953776.1"
                     /translation="MKRHLNTCYRLVWNHITGAFVVASELARARGKRGGVAVALSLAA
                     VTSLPVLAADIVVHPGETVNGGTLVNHDNQFVSGTADGVTVSTGLELGPDSDDNTGGQ
                     QIARGGTARNTTVTANGLQDVMAGGSATDTVISAGGGQNLRGQAYGTVLNGGEQWIHA
                     GGSASGTVINQSGYQTIKHGGQATGTIVNTGAEGGPESENVSSGQMVGGTAESTTINK
                     NGRQVIWSSGMARDTLIYAGGDQTVHGEAHNTRLEGGNQYVHKYGLALNTVINEGGWQ
                     VIKEGGTTAHTTINQKGKLQVNAGGKASDVTQNTGGALVTSTAATVTGTNRLGAFSVV
                     AGKADNVVLENGGRLDVLSGHTATNTRVDDGGTLDVRNGGAATTVSMGNGGVLLADSG
                     AAVSGTRSDGTAFRIGGGQADALMLEKGSSFTLNAGDTATDTTVNGGLFTARGGSLAG
                     TTTLNNGATLTLSGKTVNNDTLTIHEGDALLQGGALTGNGRVEKSGSGTLTVSNTTLT
                     QKTVNLNEGTLTLNDSTVTTDVIAQRGTTLKLTGSTVLNGAIDPTNVTLTSGATWNIP
                     DNATVQSVVDDLSHAGQIHFTSARTGKFVPTTLQVKNLNGQNGTISLRVRPDMAQNNA
                     DRLVIDGGRATGKTILNLVNAGNSGTGLATTGKGIQVVEAINGATTEEGAFVQGNMLQ
                     AGAFNYTLNRDSDESWYLRSEERYRAEVPLYASMLTQAMDYDRILAGSRSHQTGVNGE
                     NNSVRLSIQGGHLGHDNNGGIARGATPESSGSYGFVRLEGDLLRTEVAGMSLTTGVYG
                     AAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNLTHTSSGLWADIVAQGTRHSMKASSD
                     NNDFRARGRGWLGSLETGLPFSITDNLMLEPRLQYTWQGLSLDDGKDNAGYVKFGHGS
                     AQHVRAGFRLGSHNDMTFGEGTSSRDTLRDSAKHSVRELPVNWWVQPSVIRTFSSRGD
                     MSMGTAAAGSNMTFSPSQNGTSLDLQAGLEARVRENITLGVQAGYAHSINGSSAEGYN
                     SQATLNVTF"
     gene            complement(11763..12635)
                     /locus_tag="OQQ48_003090"
     CDS             complement(11763..12635)
                     /locus_tag="OQQ48_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HCS1953777.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVQRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILQCGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQEQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRAQAREQFTGAVDRIFDTAESVCVASVARTVLRAVRDT
                     VVSVARAVWNWIFF"
BASE COUNT         2891 a         3430 c         3388 g         3014 t
ORIGIN      
        1 tcggtagaat agcgttctca ggtggtcagt aaaattgtct tcaacaagat gtctgcgcat
       61 ctcgtctgct agccctacat aactgctctc gaccgaaact atttattgaa tgttcaccaa
      121 ggtgggcgtg gagacaaaat gacgtctttt ggtattagct acctgagcca catggtcaaa
      181 acgttcaagt agcatagcga aaccaccctg cccacgtaat gtggacgatg cagcttccag
      241 ccagagcaaa cttgggacca tcgactacaa aatgaagaat actgaactgc ttttctggca
      301 ggcctatata ctgcacggat atgggtgatt tcagacacaa aaaaagccgc tcttgagcga
      361 ctcgatttgc atacggtgtg gtgcgaaggc cggactcaaa catgtgactt aacctcatga
      421 tttaaaattg ttaataataa acaatatcat cttataccaa cacagatacc aacacgaaaa
      481 atcactggtt ttcggcatcg aaccaggaca atcagctcct gattgagata ccgagctctg
      541 ttactcgtca tcttcctcga caggcttcat tgcatcaagt agtactcgca ccatcgcgat
      601 agaaccaatc cggataaaag catcttctcc atctatatct gttaactgat atcgctcact
      661 tgatgccgga taattgtgtc gatttgcagc agtgaactct ttcgtacagc cagaatgcgg
      721 cggatgctgg ttgagcagat aacgtccgta ttctcgttga ccacttccgg acggtaggct
      781 gtgatatcag acataatcga tattcctttt tgcagataaa tgaaaatgcc agccctgttc
      841 aggctggcgt cagtggaatg aagtctggtg agtaagactc accggttctg gtgctgaagc
      901 tgcagttggc gcagggtacg gatatcttct ctggcctgtt cgatacaggg caacagtgct
      961 gctgaatccg ttttatcctc attgttgtca aagataattc ccgattcgca gtcgatattg
     1021 tcctgcagcc acgcaatcag aatatccagt gcggtttccg tggttaatga tttcatgttg
     1081 ttcctggtta ccagttgaaa gtgggtaaac ccggcaggca ttcagcgctg gcatccagat
     1141 gaatgagact gacgccataa cgtcgggtaa gcgtgataac cagacgacgg aacgtttttg
     1201 acagccccag gcgtttaagc cgcagaaccg gatatgacca cgcatcggta cgtaacagat
     1261 atccgctacc tgtgaaatga atccattctg attcaccaac gtcactggtc tgatgcgaca
     1321 gcgagtacag ccaggcgtta tccttttccg tgatatgtgc ggtactgcag cgtatgccgg
     1381 caagggttgc aaacggtggt agtgtgcagg ttgactgttg gtcggattcc tccaccacgc
     1441 ggagtgaata accgttttca gcgaccttgt taatcagttc agtgagatta acaccatcga
     1501 cgtcaacgat aattcgcccc atgttcagtg cctgcacatt aattctgtcg gcttccagca
     1561 tcagggaaag tttcatcgtt tctccggatg cttacccagg gtaatgttat ttaccgttct
     1621 gtaattgtcg cgggtcatca ggccggtcgc ccggcgagcc cggaggatat caatactgtt
     1681 tattaactga gagcggggac aagtgctgaa tcccggctgg tcggtacgca ccagcgcgta
     1741 tttttccacg agaaaattca ccgcatcaca cagtgaaatg ccagcttcaa tatgctgctc
     1801 aatcacacgt tcatcggcga acggtgtgtc attcagtgta aggccgtaat gctggcccag
     1861 tagtcgggag agcagagtct gccagatggt gacgggagac gggcagctcg atgcctcccg
     1921 tacgtgtgta tcgggtaatg ttttcatact gaaggttttc ctgatatgca gagatgaaaa
     1981 atggaaaagt ggcgtggtga aagcaccagg ccggagcaga aggttattcc ggtggtttac
     2041 tttttcgttt cgggcgtcgg ataaacagcc agataaacgt aaccaccgct accgagagtg
     2101 tcagctttgc aggtcagacc ttttgcatac agcgtgacgg tatgctgatg gcggggattc
     2161 agttcaccgc tggtgagcat gagttccagt tgtttcatca gtagcggaaa ggcctggtcc
     2221 aggtggtacg catctgcatc gctgaacagg cctctgatac cggcgcggtc ggcaaggtaa
     2281 tgcaggcggt taccctcctg caccagacgg gcgccgaaac agggcgtcac tgtgcagggt
     2341 agtccccacc agggatggtc gtcattgtca tcgggatgcg ttgtcccgga aagtgtgtct
     2401 gacacgataa aatcctcaca gaaaatcggt gaatgatagt ttaacgatga cgggtcagcc
     2461 ggcgcgcaga ccggcttcgg taaaggtgta ttcgttgatg agcaggacct catccacagc
     2521 ggcgtctgag ctcagccagt cgtactcgtt ttccgggtga cggaacatca gttcagggtg
     2581 acgctcatca gccggacata cggaccaaaa ctgtccttac ggcgttctgc aaacacggcc
     2641 agcacaccgg gaatatcctg cacttcacga ccggtatacg cttcagcact accgtgccag
     2701 cggtatttac cggtacagaa cggaaaaaga cgggatgcag gatgctgacg gtgaatacgc
     2761 atggcttcac cacgggtgat gattttcatg agaatagtcc tcctgaatgt gaaagtaaga
     2821 aacagctgag aaagagaaac tgaagtgata tgaaggtgat tgtgacggtg acgggttaaa
     2881 gcagaccgtg ttcggcaaag gagaaaacct ggctgccacc gactatcaga tggtccggta
     2941 cccggatatc aaccagggcc agtgcctgta ccagacgctc agtgataagg cggtctgcct
     3001 tgctgggggt gacttcaccg gacgggtgat tgtgcgccag caccacggca gcggcattgt
     3061 ggtacagggc gcgtttaatc acttcccggg gatggacttc cgtacggttg atggtgccgg
     3121 tgaagagggt ttcaccggca atcagctgat tctggttgtt cagatacagc acccggaact
     3181 cttcacgctc cagtcccgcc atgttcagaa tcagccattc ccgtgccgca tgcgtggagg
     3241 tgaaggccac gccgggttca tgaagatggc ggcccagggt tttcagggcc cgttgaatga
     3301 ggctgcgctc accgggtgtc atctctccgg gcagaaagga aagctgttgc attgtacttc
     3361 tctccattca gtcgatgatg cgcataatgg cgctgcattc cggatgctgc agggcgtaat
     3421 cccgcagccg gtaataatgg accgtcatgg cataacactc cgtacgacag gcatgatgac
     3481 tgtacgtcat cagacaggca gcaatgccgg cagcttccgg actcatttca gcgcggttac
     3541 cgttcatggc attgaacagt acccatgttt cgtcatcatc gtcatccggt tcgggtgcca
     3601 taaatgcccc gccgttgttc agggtgtaca gattccagat accaccgcag tagtcttcac
     3661 acagacggtc catccagccg aagacacggg gctccagggt cacccactgt ggaatgaggc
     3721 caaagtgctg cggccagaag ctgatgcgct gttcatcagg gacgagggtg gcaaccaact
     3781 gaggctggtc attccctgat gcagcggtta cggaaacaga agatgtggtg gaattatgtg
     3841 aaatggtttt catgaaatga ctccttaaag gacaaataaa tgcgtggttt tcagggtaat
     3901 ggctgaatca gcgcaggctc tccagcaggg tttccgccat cacccacaat gcgcggttga
     3961 gcttaatgtc agtgtcaatg ctgtgaatgg cacgggtatg gatacgtttt cctttagcgc
     4021 tgcgacctga aattccgcct ttcagcatat tctcctgaat ggtctgataa gcactccaca
     4081 ggtccttacc gtaatcctcc cggcgtcgtg gcgtcagaat gtcggcggtg gtgacgggct
     4141 gatgttcgtc accataacgg taagtcagtg ccgcctgtgc cagcgcctgg cgtgccggtg
     4201 gcggcagaat cagcgactgc atggcatcac gcttttcctc aatccggtca aacacgccca
     4261 ccacctcgta agccccttca atgactctgt ccaccacatt tccccggtgc ggaacacgca
     4321 cttcccccag agactgacca cagacgcatc cgttctggca gacgaacctg aagtaacccg
     4381 gcagcatctg gtagctggag gtaccgtcat gagagttgag cagaataatt tcagggacat
     4441 gttctccgtt tatctctccg gcccgccgca gacgcagcat gtgttttgtg tatccccggc
     4501 ggcccgggtc gcgcacacgg gtctggcagg cgaaaaaggg ctgaaaacct tcccgctgca
     4561 ggctttccag tacggtgata gtggggatgt acgtatagtt ctggcttcgg gacgtgtgtt
     4621 tatcttcccc gaaaatcccc ggcacatggt gcatcagctc ttcatgtgtc agtgggcggt
     4681 cacgacgtat ctggtttgca gcataaccaa aacgactggc taatcgcata ctttgctcct
     4741 tatcggtggt taagatttac tggtgtaata aatgaaaaaa gccgcgtctc ccggagaaga
     4801 cgcggcctga tggatgaaat caatgaggtt tattgtctga gaagccctcc actggcgagc
     4861 cacgcattaa gctgttttcc ggcatcagct tcagagttcc ggctgcgtca gcgtcgctga
     4921 ccttccgcgt tgcggataac ccgacgaaaa tgcattcccg ggtcatcctg gataaacaca
     4981 ttgcggtaag cggttatgac agcaaccgcc tgcccgtatg agaaagatcc ttcagccagg
     5041 acatactctg tgtaacccgg catatctgtt ttctcctgat aaataaaaag cctctgccac
     5101 gcgttgtgac agaggctgaa gcatgaaact gacttcaggg atcagttaac gttttttccg
     5161 gaaacggtaa tcagcagtgg atggtagtcc tggggatcga aaaccgataa cggcagactg
     5221 acatgatggc cgttactttc ttcagttgct ttaatgattt cggttgtggc gacattttcc
     5281 acgcactccg tttccagaaa tgcgtctgtg gttcgcgtgg cattactgtc accaaaggct
     5341 tccgtttcca tttttctggt caccagcgtc tgaccatatt tgtctttgag ttgcagagtg
     5401 atggtgaggg ggccaaatcc ttcatcgttt ccgccattat ccagccggaa ctggtaggca
     5461 caaatatttc ccgggagcca tatcgtatct gtattgcgta tactgatgta acgttgatcc
     5521 tgtgcccgga gtggggcaga ccacgttaac cccagaatga aggcggtaat catgcaggtt
     5581 ttgaacaggt gaatcatggt atttacctct ctgagtcatg acgatgacac tgacaaatca
     5641 ggtgataaaa cgtaaaaggc gcagaatagc cgttatgccg gtaactccgg ggggaatgct
     5701 tcttccagtc ggttaaccat attgccgaga tgggatgcat catattccat gacggggcgt
     5761 tgcctgatga tactgaccac cagtggtttg attaacatat tggtcgcggc ccgttgttgt
     5821 ataccggcgg cgaaaatgat ctccagagca ttctgatcga taacacgggc cagattctcg
     5881 ttcagatttt caatctcatg tccggtcagc aggaaactgc cggcaagcca tgtcacctgc
     5941 tcctgaatca ttgccagaat tttgacgcga tcttcttcaa ccagcgctcc ggcaattttt
     6001 cctgatacag cggaggcaat cattcccccc agaacaccgc caaccacacg ccctgtcagt
     6061 gcaccaaatg gtcccagtgg actgaacagt attccgcccg ccactgaccc gacagcacca
     6121 cctgccacac cggaagatgc cacggcaaga ttcctgatga actgcgcacc ggagatccgt
     6181 ccccgcaaca ttttgatcat gtcagggccg gttgtgacag ctaccagtgc cagagatgtc
     6241 accagcgtac ccttcattac tttgttcagg gcactgatat ttcctgcacc ggtccccttc
     6301 tgaagagcca gtcggacagt cgggctggca gtcgaaaaat caatgtgctt cagcataccc
     6361 tgaacaacac tgagccggtg gagttgctgg gttgtgacgt agacagccag tgtgcgggtg
     6421 aaggtttttc ctgcctggac agcagctgtc tgcagggcag catcgcgatc gccggtgctg
     6481 agccagaaga ccgatgccgt cagggcaaaa ctgatccctc cggccgccag actgataacc
     6541 gacccctcgg caatatcata agtgaccgat tcgatggtcc cgaaccgggt gatattacgg
     6601 gcctgggtat aagtcagatg tccccgacga atcagccggg acgcttctgc gggatcggtt
     6661 acaccgggta ctttaccttc gcggatctta ttcttcatgg tttccacagc gccggcatac
     6721 tgatcacggg ggacttccag ttgcatggga ccatgatttc ccatgtaacg atactgtccg
     6781 ttttgtccgt cgaatgccgc accgacgcta cgggctgcag ttgaacaata tttcgtctgg
     6841 atttctgttc cgctgaccag tctgtctgct ccatttctgg cattattatc accgaggatc
     6901 cgtgcgtttt tcagacggac tctgtcgaca atatggtttc cccgttcagc ggcaaaccca
     6961 tgtccctgcc ctccggaagc aaacattttc tcatttgtac tccaggtttc cggcagtttc
     7021 atgagaatca gaccattcgc cgttgcatcg gaaataaaac cggtgtaaga acgttcagaa
     7081 atgtcctgac acagtgtttc tgtttcgcga caaagagagg agcagtacca ttccggatcc
     7141 ggcttatgat tttccggggc atgatgtact tctgtcagcc cgcaggaggc acagatacga
     7201 agctcatcca cgtggtggaa tgatacaaaa cggcggcctt ctgaaaaggg gtaccagtca
     7261 ccgccgtcca ggcaacgatc gagaaaatcg tcattcgctt ccggtgataa attcaggcgg
     7321 ttccggaaga gcgttttcag ttcatcaggt gtgaatgaca ggttctcttt caactgttcg
     7381 ttaaaccctg ccatgtttct ttcataccag gcattgtcca gtagccagtt tccgtctgcc
     7441 agttcaaatt ccctcagttc tccccgggaa atccgttgtg aaatgagtgt cagccagatg
     7501 cgctccgtca gcgtttcagg aaggctgtaa tcgttttgca aactgctcag ccacgattta
     7561 ctgttgagca ccggttgttc ttccagtgtc tggctgatgg cactggtcag tcttttcagc
     7621 gcctggatct ggctggccgc ccatagcagc gtcactaatg cagccagcca gtacaacaac
     7681 ccgccccacc acatcagtga gggtgaaata tcgaactgca atgcgatcaa tggtgcgtat
     7741 gcagaacacg ccataaggaa aaaagcagta taaaaacagc agtaataccc gttaatttcc
     7801 ttgctgatat cttcaggtgt gagcccgctc tgctgaatgg ttcttatctg cacgggaaga
     7861 accagcagac cagcggcgag agcggtggtc agaataaatt ttacagagtc aaaagtatga
     7921 gcctccagtg cgatatgttt accatcaagt ccccacatga agctgatgat cattccggca
     7981 atcgtcagaa atacgcctgc cagccagcga atatggaata accgggttat gcgttcagtg
     8041 atatagccag ccagtaaagc cggaccgaac agacacagca atatgaaccc tgccagcgca
     8101 gacgctgtgc tggttaatgc tctgaacaaa aggcgaagaa tggcaaatcc tgcgatcagc
     8161 agaatcagcg cgccgactat ctgtaacata gtcactccgt gatgaatatc atgttattgt
     8221 gaatgccagt gaatgtggca ctgaaacgtt ttcccccgtc cgggtcccgg tcatgatgac
     8281 cgggaccaca gagaggcaat gcagaattat cagaaggtca cattcagcgt ggcctgactg
     8341 ttataacctt cagcgctgct gccgttgatg ctgtgggcat aaccggcctg aacgcccagg
     8401 gtgatgtttt cccggacacg ggcttccagt ccggcctgca ggtccagtga cgtaccattc
     8461 tgtgacggtg agaacgtcat gttactgccg gctgcggctg tacccatgct catgtccccc
     8521 cgggaactga aggtgcggat aacagaaggc tgtacccacc agttcaccgg cagttcacgc
     8581 acactgtgtt ttgcactgtc gcgcagggtg tcacgggatg aggtgccttc accaaaggtc
     8641 atatcgttgt ggctgcccag gcggaaaccg gcacgcacat gttgtgcact gccatgcccg
     8701 aacttcacat aaccggcgtt gtccttaccg tcatccaggg agagcccctg ccaggtgtac
     8761 tgcagtcgtg gctccagcat caggttgtca gtgatactga agggcagacc ggtttccagt
     8821 gaacccagcc agccccggcc ccgggcgcgg aagtcgttat tgtccgatga tgctttcatg
     8881 ctgtggcggg ttccctgtgc cacaatgtca gcccacaggc cggaggatgt atgtgtcaga
     8941 ttcaggtatc cgcccaggct gccggcatca tcccggaccg tgccggcgcg ggagccgtca
     9001 tcatccttaa catcaacgga agaatggcct gcagcaccat acactcctgt cgtcagtgac
     9061 ataccggcga cctctgttct gagcaggtca ccctccagac ggacgaagcc atagctgccg
     9121 ctgctttccg gcgtggcccc acgggcaata ccgccgttgt tatcgtgacc gagatggccg
     9181 ccctgaatgc tgagacggac gctgttattt tcaccgttta caccggtctg atggctgcgt
     9241 gagcctgcca gaatccggtc atagtccatt gcctgtgtca gcatggaggc gtacaggggg
     9301 acttcagcac gataacgttc ttcactgcgc agataccagc tctcatcact gtcccggttg
     9361 agggtgtagt taaaggcccc ggcctgcagc atattcccct ggacaaaagc cccttcctcc
     9421 gtggtggcac cgttaatggc ctcaaccacc tgaataccct taccggtggt cgccagcccc
     9481 gtaccactgt tgccggcgtt caccagattc aggatggttt ttccggttgc cctgccaccg
     9541 tcaatgacca gtctgtcagc attgttctgc gccatatccg ggcgtacacg caggctgatg
     9601 gtgccattct gcccgttcag gtttttcacc tgcagagttg tcggaacgaa cttccctgtg
     9661 cgggcggagg tgaaatgaat ctgtccggca tggctgaggt catccaccac cgactgaacc
     9721 gtggcgttat cggggatatt ccaggtggca ccggatgtca gggtgacatt cgtggggtca
     9781 atggcaccgt tcagcactgt gctgccggtc agcttcaggg ttgtaccgcg ctgagcaatg
     9841 acatccgtgg tgacggtact gtcgttcagc gtcagcgtgc cttcattcag gttgacggtt
     9901 ttctgggtga gtgtggtgtt gctgacagtg agtgtgccac ttcctgattt ttccaccctg
     9961 ccgttaccgg taagagcgcc tccctgcagg agtgcatcac cttcatggat ggtcagggta
    10021 tcgttattca ccgttttccc ggaaagagta agcgtggcac cgttattcag cgtggtggtg
    10081 cccgccagcg agccccctct ggcagtgaac agtccgccat ttaccgtggt atccgtggcc
    10141 gtatcaccgg cgttcagcgt gaatgaactg cctttttcca gcatcagggc atccgcctga
    10201 ccgcctccga tacggaatgc cgttccgtcg ctccgggtac cactgacagc ggccccggaa
    10261 tcggccagca gtacaccgcc attccccatg gatacggtgg tggcggcacc accgttgcgg
    10321 acatccagcg ttccgccatc atccacacgg gtattagtgg ctgtgtgtcc gctcagcaca
    10381 tccagacgtc cgccattttc cagtacgaca ttatcagcct tacccgccac aacggagaat
    10441 gctcccaggc ggtttgtgcc ggtgacggtt gcagctgtgc tggtaaccag tgcacctccc
    10501 gtgttctggg tgacatcaga cgctttaccg ccggcattca cctgcagctt tcctttctgg
    10561 ttgatggtgg tatgcgcggt agttcctcct tccttaatca cctgccagcc cccctcgtta
    10621 attaccgtgt tcagcgcaag tccgtatttg tgcacatact ggtttcctcc ctccagccgg
    10681 gtgttatgtg cctcgccgtg taccgtctgg tcaccgccgg cgtaaatgag ggtgtcccgt
    10741 gccatccccg aagaccagat aacctgccgg ccatttttgt tgatggtggt ggattcagcc
    10801 gtccctccaa ccatctgacc gctggacaca ttttcagact ccggaccacc ttctgcaccg
    10861 gtgttgacga tggttccggt tgcctgcccg ccatgtttaa tggtctggta gccagactgg
    10921 ttaatcaccg ttccggatgc actgccgcct gcatgtatcc attgttcacc accgttaagg
    10981 acagtaccgt aagcctgccc gcgcagattc tgtcctccac cggcactgat aaccgtatcc
    11041 gtggcactgc cacctgccat gacatcctgc agaccgtttg cggtgaccgt ggtgtttctg
    11101 gcagtgccac cccgggctat ctgctgaccg ccggtgttgt catcagagtc cggccccagc
    11161 tcaagcccgg tactgacagt cacgccatca gctgttccgg atacaaactg gttgtcatgg
    11221 tttaccagtg ttccgccatt cactgtttca cccgggtgca caacgatgtc agcagccagc
    11281 accgggagtg acgtgactgc ggcaagagac agtgcaaccg ccacaccgcc acgtttaccc
    11341 cgtgcgcggg ccagttcgga ggcaaccacg aaagcgcccg taatgtgatt ccataccagc
    11401 ctgtagcagg tattcagatg tcgtttcatc agcatttcct taatcagagt gagggtgaat
    11461 aaaaaagccg gtatccacag atgtggatac cggcaggtaa agggttacag tgccggaggt
    11521 ccggctgacg gaagtttctg acgtgtggct ctgttgttcc ctgaatctca tgtctgtgtg
    11581 tatctgcgca cgttgctaac aggagttatt attagcataa cgatcgatat aaacgatcga
    11641 taaaatggat atcgatcgtt ttattctatt atcaggctat tgcacggaca agagagacag
    11701 taacgaaaac agcgcttttg tgagcagact cagaggcagg gaggacatca tccacaacag
    11761 gttcagaaga agatccagtt ccataccgcg cgggcaacag agaccaccgt gtcacggacg
    11821 gcacgcagaa ccgtacgggc aacagaggca acacagacgc tctccgccgt gtcaaatatc
    11881 cggtccaccg caccggtaaa ctgttcacgg gcctgagcgc ggacagattc cgtgcgcagc
    11941 tcgtcctgca ggcgggtcat caggggactg gcggcatggt cgggaagtgc tgtcattagc
    12001 gcactgacca gcgtatccag ttcccagccg gtgcgggccg atacggcaac aaccggatgt
    12061 acgggccgga acagacggaa taccgcctcc gttttctcac gaatgttctg ttcctgtgcg
    12121 ggagaaggct gaatacctgc catatcccat tcatggcagg gttccgtttt gtcagcctgc
    12181 gtcaccacaa acagcacctg ctgatgtccg cactgcagga tgtgtcgcca gaaatactca
    12241 tccacagaca gggcccggtc atcggcttta atcagccaca gcaccaggtc cagttcaggc
    12301 agaatgtcac ggtacagggc ttcatactct gcatccctgt cccggctctc gcccaccccg
    12361 ggcaggtcag tgataaccat gctgtgaccg tgaccactca gacggaagcg ctgcacttcc
    12421 cgggtgccgg cgtgaacatc actgaccggt gtgacctccc cctgaaacag tgcattacag
    12481 agtgaagatt taccggcccc gcttttaccc ataatgccaa tcacgggttc gtgactggtg
    12541 agtttgcgca gatgttccag gatgtgacgg gaaagtgagt aaggcaggga ggagagcggt
    12601 ttttcaattg cctcaatggc atcagacgga ttcatacatg tattcccggt gaaaaaagac
    12661 aaaaccccgc gcaccggaaa ggtggcgggg gattcacgga gataatatct gtctgagatt
    12721 att
//
LOCUS       DAJVUJ010000124        12697 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.124, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000124 DAJVUJ010000000
VERSION     DAJVUJ010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12697
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.124"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..1365
                     /locus_tag="OQQ48_003091"
     CDS             <1..1365
                     /locus_tag="OQQ48_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HCS1953778.1"
                     /translation="AKKFGIDIIPDYTFNFMGIGGSGKNDLDYPSADIRAKISKDIES
                     GIPGYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEASKDNISKDEWENLHALKE
                     KRLNGMPKTTPKSGQIIMLQNQYVREMRKYGVRGLRYDAAKHSKHEQIERSITPPLKN
                     YNERLHNTNLFNPKYQKKAVMNYMEYLVTCQLDEQQMSSLLYERDDLSAIDFSLLMKT
                     IKAFSFGGDLQTLASKPGSTISSIPSERRILININHDFPNNGNLFNDFLFNHQQDEQL
                     AMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGSTRVGGEAWLNKGCSTYQQLYNEFH
                     ALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVIINKGNEEVEGTWPNK
                     LQPGIYKNMGSNSVNIIINNTRKIIPPGKVFTLRGGTLNINIPGRSALLLGKTGEPPN
                     YLYL"
     gene            1701..2678
                     /gene="csiD"
                     /locus_tag="OQQ48_003092"
     CDS             1701..2678
                     /gene="csiD"
                     /locus_tag="OQQ48_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HCS1953779.1"
                     /translation="MNALIAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKPLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            2698..3966
                     /gene="lhgO"
                     /locus_tag="OQQ48_003093"
     CDS             2698..3966
                     /gene="lhgO"
                     /locus_tag="OQQ48_003093"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HCS1953780.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNDIRYDNCGKMLIATSDLEMERM
                     RALWERTAANGIEREWLNAEELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVVRTRQGGEYEASTLISCSGLMANRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRATRSVDALHAAFNQ"
     gene            3989..5424
                     /gene="gabD"
                     /locus_tag="OQQ48_003094"
                     /pseudo
     CDS             3989..5424
                     /gene="gabD"
                     /locus_tag="OQQ48_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
     gene            5438..6704
                     /gene="gabT"
                     /locus_tag="OQQ48_003095"
                     /pseudo
     CDS             5438..6704
                     /gene="gabT"
                     /locus_tag="OQQ48_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417148.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
     gene            6942..8303
                     /gene="gabP"
                     /locus_tag="OQQ48_003096"
     CDS             6942..8303
                     /gene="gabP"
                     /locus_tag="OQQ48_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005114089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HCS1953781.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYVPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTDLLAIGIICTVPIMAA
                     GIS"
     gene            8362..9024
                     /gene="csiR"
                     /locus_tag="OQQ48_003097"
     CDS             8362..9024
                     /gene="csiR"
                     /locus_tag="OQQ48_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HCS1953782.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGSDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(9025..9474)
                     /gene="kbp"
                     /locus_tag="OQQ48_003098"
     CDS             complement(9025..9474)
                     /gene="kbp"
                     /locus_tag="OQQ48_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HCS1953783.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(9558..9716)
                     /locus_tag="OQQ48_003099"
     CDS             complement(9558..9716)
                     /locus_tag="OQQ48_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HCS1953784.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            9899..10198
                     /locus_tag="OQQ48_003100"
     CDS             9899..10198
                     /locus_tag="OQQ48_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS1953785.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            10208..10732
                     /gene="ygaP"
                     /locus_tag="OQQ48_003101"
     CDS             10208..10732
                     /gene="ygaP"
                     /locus_tag="OQQ48_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HCS1953786.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDRIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(10779..11183)
                     /gene="stpA"
                     /locus_tag="OQQ48_003102"
     CDS             complement(10779..11183)
                     /gene="stpA"
                     /locus_tag="OQQ48_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HCS1953787.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            11850..12299
                     /gene="alaE"
                     /locus_tag="OQQ48_003103"
     CDS             11850..12299
                     /gene="alaE"
                     /locus_tag="OQQ48_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HCS1953788.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(12336..12680)
                     /locus_tag="OQQ48_003104"
     CDS             complement(12336..12680)
                     /locus_tag="OQQ48_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HCS1953789.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
BASE COUNT         3156 a         3116 c         3249 g         3176 t
ORIGIN      
        1 gctaaaaaat ttggtatcga tatcattcct gattatacat ttaactttat gggaatcggg
       61 ggtagtggta aaaatgacct ggattatccc tctgctgata tacgagcgaa gatcagtaaa
      121 gatatagaaa gcggtatccc tggctattgg caaggtcagg ttttgattcc attcattaaa
      181 gatccagtaa caaaagaacg taaacaaatc catccagaag atatacatct cactgcaaaa
      241 gacttcgaag caagtaaaga taacatctct aaggatgaat gggaaaacct ccatgcatta
      301 aaagaaaagc gtttaaatgg aatgcctaaa acaacaccca aaagtggcca gattattatg
      361 ttgcaaaatc aatacgttcg agaaatgcga aaatatggcg tacgaggttt acgttatgat
      421 gcggcaaaac actcaaaaca tgaacaaata gagaggtcga taaccccacc gcttaaaaat
      481 tataatgagc ggttacacaa tactaattta tttaacccaa aatatcaaaa aaaagccgtt
      541 atgaattaca tggaatacct ggtaacttgt cagttggatg aacaacaaat gtcatcactg
      601 ctttatgaga gagatgattt aagtgccatt gatttttctt tactcatgaa aacgataaaa
      661 gccttttcat ttggtggaga tctccaaacc cttgcatcaa aaccgggttc cacaatctca
      721 agtatcccat cagaaagacg gatattgatt aacattaacc acgattttcc taacaatggt
      781 aatcttttca atgactttct atttaaccat caacaagatg aacaattagc aatggcatat
      841 atggccgctc tcccgttcag cagaccttta gtttactggg atggccaagt attaaaatca
      901 acgactgaaa ttaaaaatta tgatgggtcc acgcgtgtcg gcggtgaggc gtggcttaat
      961 aaaggttgct ctacctatca gcagctctac aatgaattcc acgcattata tatagataaa
     1021 gcaggaatat ggagcgcatt tgagggtgta tttgcaacta aaaacgttct ggcctttagt
     1081 cgtggggatt ctgtgaacat taatcactct cctcatgatg ggctagttat aataaataaa
     1141 ggaaacgaag aagttgaagg tacctggcct aacaaattgc aacctggaat atacaaaaac
     1201 atggggagta atagcgttaa cattattatt aataataccc gaaaaattat cccccctggt
     1261 aaagtattta cgcttagagg cggaactcta aatatcaata ttcctgggcg tagcgctctt
     1321 cttttaggga aaacaggaga accgccgaac tatctctatt tataatttaa tgttatatct
     1381 gccccgataa aacggggcag ataatatgtt tagtttacta acggtcattt tgcagtgaag
     1441 ccatttactg ttttttatcg accagataat ctgttctcta atgttaactc cccctaacct
     1501 gttgctttag ttattcattt cctgtctcac tttgccttaa taccctacgt taaatgttac
     1561 taatttgttg tttttgatca caataagaaa acaatatgtc gcttttgtgc gcatttttca
     1621 gaaatgtaga tatttttaga ttatggctac gaaatgagca tcgccatgtc accctacatc
     1681 tcataagagg atcgcttctg atgaatgcac tgatcgccgt acaaaataac gctgtcgatt
     1741 caggccagga ctatagcgga ttcactctca tcccgtcggc gcaatccccg cgtctgctgg
     1801 aactcacctt caccgaacag acgaccaaac agtttctcga acaggttgcc gaatggcccg
     1861 tgcaggcgct ggagtacaaa tcgtttctgc gttttcgggt aggcaaaatt cttgacgatc
     1921 tgtgtgcgaa tcagctgcaa ccattgctgt tgaagcccct gctaaaccgt gctgaaggtg
     1981 cgctgttgat caatgcggtg ggtatcgatg atgtggcgca ggcagatgag atggtgaagc
     2041 tggcaacggc ggtagcgcat ctgattggtc gctcaaattt cgatgcgatg agcggtcagt
     2101 attacgcgcg tttcgtggtg aaaaatgtcg ataactcaga cagctatctg cgtcagccgc
     2161 accgcgtgat ggaactgcac aacgacggca cgtatgtcga agagatcacc gattacgtgc
     2221 tgatgatgaa aatcgatgag caaaacatgc agggtggaaa ttcgttgctg ctgcatctcg
     2281 atgactggga acatctggac cactatttcc gccatccact ggcgcgtcgc ccgatgcgct
     2341 ttgccgcgcc gccgagcaaa aacgtcagca aagatgtttt ccatccagtg ttcgacgttg
     2401 atcaacaggg tcgcccggtg atgcgctata tcgaccagtt cgtccagcca aaagattttg
     2461 aagaaggcgt gtggttgagc gagctttccg atgccattga aaccagcaaa ggcattcttt
     2521 ctgtacccgt tcccgttggc aaattcctgt tgataaacaa cctgttctgg ctgcacggtc
     2581 gcgaccgctt tactccgcac ccggatctgc gccgtgaact gatgcgtcag cgtggctatt
     2641 tcgcttacgc cactcaccac taccagacgc atcagtaagc gcaaaggaat tgagcggatg
     2701 tatgattttg tgattattgg cggcggcatc atcggcatgt cgaccgccat gcaactgatt
     2761 gatgtttatc cggatgcccg cattgcgttg ctggaaaaag agtccggccc ggcctgtcac
     2821 cagacgggcc acaacagcgg cgtgatccat gccggggtct attacacgcc cggcagcctg
     2881 aaggcgcagt tttgcctggc gggaaaccgc gccaccaaag ccttttgcga tcaaaacgac
     2941 attcgctatg acaactgcgg caagatgctg atcgccacgt ccgatctcga aatggaacgg
     3001 atgcgcgcgt tgtgggaacg cacggcggcg aacggtatcg agcgcgagtg gttaaacgcc
     3061 gaggaactgc gcgagcgcga accgaatatc accgggctcg gcggcatttt tgtgccgtcc
     3121 agcggcattg tcagctatcg cgaagtcaca gcggcaatgg caaaaatctt ccaggccaga
     3181 ggcggtgaga ttatttataa cgccgaagtc agcgccctca gtgagcataa aaacggcgtg
     3241 gtcgtccgaa cccgtcaggg cggcgaatat gaagcatcga cgctgattag ctgttccggg
     3301 ctgatggcta accggctggt gaaaatgctc ggcctcgaac cgggttttat tatctgcccg
     3361 ttccgtggcg agtatttccg ccttgcgccg gagcataacc agattgttaa ccatctgatt
     3421 taccccatcc ctgacccggc aatgccgttt ttgggcgtgc atctcacccg catgatcgac
     3481 ggcagcgtga ccgtcgggcc aaacgcggtg ctggctttta aacgcgaagg ctatcgcaag
     3541 cgcgacttct catttagcga cacgctggaa attttaggct cgtcggggat tcgccgggtg
     3601 ctgcaaaacc atctacgctc aggactgggc gagatgaaaa actcgctgtg caaaagcggc
     3661 tatctgcggc tggtgcaaaa gtattgtccc cggctttcgt taagcgatct ccagccctgg
     3721 cccgccggtg tgcgggcgca ggcggtatcg ccggacggca agctgattga cgattttctg
     3781 tttgtcacca ccccgcgcac gatccacacc tgcaatgcgc cctccccggc agcgacatca
     3841 gcaattccta ttggtgcgca tattgtcagc aaggtacaaa cgctgttggc aagccagagt
     3901 aaccccggac gcacgctgcg agcgacacgt agtgtggatg ccttacacgc cgcatttaat
     3961 caataacctt tgaaaacagg acgtaacaat gaaacttaac gacagtaact tattccgcca
     4021 gcaggcgttg attaacgggg aatggctgga cgccaacaat ggcgggatca tcgacgtcac
     4081 caatccggcg aacggcgaca agctgggtag cgtgccgaaa atgggcgctg atgaaacccg
     4141 cgccgctatc gacgccgcca accgcgccct gcccgccagg cgtgcgctca ccgccaaaga
     4201 acgcgccaac attctgcgca actggttcaa tttgatgatg gagcatcagg acgatttagc
     4261 gcgcctgatg accctcgaac agggcaaacc actggccgaa gcgaaaggcg aaatcagcta
     4321 cgctgcctct tttattgagt ggtttgccga agaaggcaaa cgtatttatg gcgataccat
     4381 tcccggtcat caggccgata agcgcctgat tgttatcaag cagccgattg gcgttaccgc
     4441 agctatcacg ccgtggaact tcccggcggc gatgattacc cgtaaagccg gtccggcgct
     4501 ggcggcaggc tgcaccatgg tgctgaagcc cgccagtcag acaccgttct ctgcgctggc
     4561 gctggcggag ctggcgattc gcgcgggcat tgcggctggc gtgtttaacg tggtcaccgg
     4621 ttcggcaggc gcggtcggta atgaactgac cagcaacccg ctggtacgca aactgtcgtt
     4681 taccggttcg accgaaattg gccgacagtt aatggaacag tgcgcgaaag acatcaaaaa
     4741 agtgtcgctg gagctgggcg gcaacgcgcc gtttatcgtc tttgacgatg ccgatctcga
     4801 taaagccgtg gaaggcgcgc tggcctcgaa attccgcaac gccgggcaaa cctgcgtctg
     4861 cgccaaccgt ttggcgtgta tgaccgcttt gccgaaaaat tgcagcaggc ggtgagcaaa
     4921 ctgcacatcg gcgacgggct ggataaaggc gtcaccatcg ggccgctgat cgatgaaaaa
     4981 gcggtagcaa aagtggaaga gcatattgcc gatgcgctgg agaaaggtgc gcgtgtggtt
     5041 tgcggcgcta aagcggatga acgcggcggc aacttcttcc agccgaccat tctggtggac
     5101 gtgccggcca acgccaaagt gtcgaaagaa gagacgttcg gccccctcgc cccgctgttc
     5161 cgctttaaag atgaagccga tgtgattgcg caagccaatg acaccgagtt tggccttgcc
     5221 gcctatttct acgcccgtga tttaagccgc gtcttccgcg tgggcgaagc gctggagtac
     5281 ggcatcgtcg gcatcaatac cggcattatt tccaatgaag tggccccgtt cggcggcatc
     5341 aaagcctcgg gtctgggtcg tgaaggttcg aagtatggca tcgaagatta cttagaaatc
     5401 aaatatatgt gcatcggtct ttaactggag aatgcgaatg aacagcaata aagagttaat
     5461 gcagcgccgc agtcaggcga ttccccgtgg cgttgggcaa attcacccga ttttcgctga
     5521 ccgcgcggaa aactgccggg tgtgggacgt tgaaggccgt gagtatcttg atttcgcggg
     5581 cgggattgcg gtgctcaata ccgggcacct gcatccgaag gtggtggccg cggtggaagc
     5641 gcagttgaaa aaactgtcgc acacctgctt ccaagtgctg gcttacgagc cgtatctgga
     5701 gctgtgcgag attatgaatc agaaggtgcc gggcgatttc gccaagaaaa cgctgctggt
     5761 tacgaccggt tccgaagcgg tggaaaacgc ggtaaaaatc gcccgcgccg ccaccaaacg
     5821 tagcggcacc atcgctttta gcggcgcgta tcacgggcgc acgcattaca cgctggcgct
     5881 gaccggcaag gtgaatccgt actctgcggg catggggctg atgccgggtc atgtttatcg
     5941 cgcgctttat ccttgcccgc tgcacggcat aagcgaggat gacgctatcg ccagcatcca
     6001 ccggatcttc aaaaatgatg ccgcgccgga agatatcgcc gccatcgtga ttgagccggt
     6061 tcagggcgaa ggcggtttct acgccgcgtc gccagccttt atgcagcgtt tacgcgctct
     6121 gtgtgacgag cacgggatca tgctgattgc cgatgaagtg cagagcggcg cggggcgtac
     6181 cggcacgctg tttgcggtgg agcagatggg cgtggcgccg gatcttacca cctttgcgaa
     6241 atcgatcgct ggcgggcgtc accgggcgcg cggaagtgat ggacgctgtt gcaccgggcg
     6301 gtctgggcgg cacctatgcc ggtaacccga ttgcctgcgt ggctgcgctg gaagtgttga
     6361 aggtgttcga gcaggaaaat ctgctgcaaa aagccaacga tctggggcag aagctaaaag
     6421 acggattgtt ggcgatagcc gaaaaacacc cggagatcgg cgacgtacgc gggctgggag
     6481 cgatgattgc catcgagctg tttgaagatg gcgatcacag caaaccggac gcaaaactca
     6541 ccgccgggat cgtggcacgc gcccgcgata agggcctgat tcttctctcc tgcggcccgt
     6601 attacaacgt gctgcgcatc cttgtactgc tcaccattga agacgctcag atccgtcagg
     6661 gtctggagat tatcggtcag tgttttgatg aggcgaagca gtagcaccgt ccccatgcag
     6721 gaggcgacgc tgcgcgtctt gtccggccta cgagaatcca ggtcggataa ggcgtttacg
     6781 ccgcatccga caatctgtac gtgaacagga ataaatctat gtcggccgga taatgcggag
     6841 ccgctctccg gcaaaaagaa tcaataacaa ttaaacgcgt gacccggcgc gggaaatgtc
     6901 ggggcgctct cccaagtgac acactttcga gaggattcag gatggggcaa tcatcgcaac
     6961 cacatgagtt aggcggcggg ctgaaatcac gccacgtcac catgttgtct attgccggtg
     7021 ttatcggcgc aagtctgttt gtcggttcca gcgtcgccat cgccgaagcg ggcccggcag
     7081 tattactggc ctatctgttc gccgggctac tggtggttat gattatgcgg atgttggcgg
     7141 aaatggcggt tgccacgccc gataccggtt cgttttccac ctatgccgat aaagccattg
     7201 gtcgctgggc gggctatacc atcggctggt tgtactggtg gttttgggta ctggttatcc
     7261 cgctggaagc caacatcgcc gccatgatcc tgcactcgtg ggttccaggc attcccatct
     7321 ggttattttc cctcgtcatt acccttgcct taactggcag taatttatta agcgttaaaa
     7381 actacggcga atttgagttc tggctggcgc tgtgcaaagt catcgctatc ctggccttta
     7441 ttttccttgg tgcagtcgca attagcggtt tttaccctta tgccgaagtg agcgggatct
     7501 caagattgtg ggatagcggc ggctttatgc ccaacggctt cggtgcggta ttaagcgcga
     7561 tgttgatcac catgttctcg tttatgggcg cagaaattgt caccattgcc gccgcggaat
     7621 ccgacacgcc ggaaaaacat attgtccgcg ccactaactc ggttatctgg cgtatttcta
     7681 tcttctattt gtgctctatt tttgtcgtcg tggcattaat tccgtggaat atgcccggac
     7741 taaaagccgt cggttcttat cgctcggtac tggaattgct caatattccc catgcgaaat
     7801 taatcatgga ctgtgtgata ttactttccg taaccagctg cctgaactcg gcgctgtata
     7861 ccgcttccag gatgctctac tccttaagcc gtcgcggaga tgcgcctgcg gtaatgggta
     7921 aaatcaaccg cagtaaaacc ccgtacgtgg cggtgttact ctccaccggc gcggcattct
     7981 taacggtggt ggtgaactat tacgtgcctg cgaaggtatt taaatttctg atcgacagct
     8041 ccggcgctat cgccctgctg gtttatttag tcatcgccgt ttcacagttg cggatgcgca
     8101 aaattctgcg agcagaagga agcgaaattc gcttgcgcat gtggctttat ccctggctca
     8161 cctggctggt catcggcttt attacctttg tgttggtagt gatgctattc cgcccggcgc
     8221 aacagttaga agtgatctcc accgacttat tagcaatagg gattatctgt accgtgccaa
     8281 ttatggcggc tggaataagc tgataatgtg gcaaaaaaca cccgttcata atacgcgctg
     8341 ataataatca ggagtcacac catgaccatt acgtctctgg atggctatcg ctggctgaag
     8401 aacgatatta ttcgcggtaa ttttcaaccg gatgaaaaat tacggatgag tttgctgaca
     8461 tcgcgttatg cacttggcgt tgggccgttg cgggaagcac tttctcaact ggtggcggaa
     8521 cggctggtca cggtggtgaa tcaaaaaggg tatcgggtgg cgtctatgtc agagcaggag
     8581 ctgctcgata ttttcgatgc ccgcgccaat atggaagcga tgttagtgag tctggcgatt
     8641 gcccgcggta gcgatgagtg ggaggcagac gttctcgcaa aagcgcatct gctcagtaag
     8701 ctggaagcct gtgacgccag cgagaaaatg cttgatgagt gggatctgcg tcatcaggcg
     8761 tttcatacag caattgtggc gggctgtggt tctcactatt tgctgcaaat gcgtgaacgg
     8821 ttgtttgatc tggcggcgcg ctatcgattt atctggctgc ggcgaacggt gctttcggta
     8881 gaaatgctgg aggataaaca cgatcagcac cagaccctga ctgcggcggt actggcgcga
     8941 gataccgcgc gcgccagtga gttaatgcgc cagcatttac tgacgccaat tcccattatc
     9001 cagcaggcga tggctggcaa ttaactactc ttccggaata cgcaacactt gccccggata
     9061 aattttatcc gggcttttta gcatcggttt attcgcttcg aagattttat tgtacagatt
     9121 agcgttaccg tagacctgtt tggaaatggc actcagagtg tcgccagact taacggtata
     9181 aaactggctg gcagtggctg gtgtcgccgt tttcacctga tcatcaacac tggcaatacc
     9241 ggaaatattc cccaccgcaa caaggatttt ctccttcgcc tcctgactca ggccgtcacc
     9301 agtgaccgtc gctttgccgt cggcaatttg aatgttcact ttatcggcat caggtatacc
     9361 ggttttgctc agatgctcct gcaccttctt cgcctgatcg tctttatcgt gctgacctgt
     9421 aaccgcgtcc cagagttttt ctccggcatc tttcacaaaa ttgaacagac ccatagctac
     9481 ctcattcgtt aacggaaaca ctcagaaagt gtagcagagg aacgcggcgc gttaacgccg
     9541 cgggggggag cacaggttta atcgcgggtt tgcacccaga acgcgtgaat caaaccgggg
     9601 atatacccaa gcagtgtcag cagaatatta ataatgaacg cccaaccgaa ccctttaccg
     9661 agcagcacgc cgagcggcgg cagaatgatg gtgatgacga ttctccagaa acccatatgt
     9721 actccctata agaaaattac tcattgttta aaaagagatt ttatctctta agcgtagtaa
     9781 ttttagcgga gcctgccagt ttcagcttac gcttttactc tcatttacgc tgctgaggct
     9841 ggtatatgtt gcatttttag tttagagttt acttaattta gaaaatactt aaataaatat
     9901 gactgaactc gcgcaattac aggccagtgc cgaacaggca gcggccttat tgaaagcaat
     9961 gagccatcct aaacgattgc tgattctgtg catgcttagc ggttcccccg gcaccagcgc
    10021 gggagagctg acgcgcatta ccggactgag tgcctctgcg acatcacagc atctcgctcg
    10081 tatgcgggac gaagggctta tcgacagcca acgggatgcc caacgcattc tatattccat
    10141 taaaaatgag gcggtaaatg ccattatcgc caccctgaaa aatgtctatt gtccgtaagg
    10201 agtcactatg gctttgacaa ccatttcgcc gcatgatgca caagaattaa tcgcacgcgg
    10261 cgcaaagtta atcgatattc gtgacgctga tgaatatctt cgtgaacata ttcctgaagc
    10321 agatctggct ccattatccg tgctggaaca gtcaggtctt ccacctaaat tacgtcgcga
    10381 gcaaattata ttccactgcc aggcaggtaa acgcaccagt aataacgccg ataaattagc
    10441 agcgattgcc gcccccgcag aaatattttt actcgaagat aggattgatg gctggaaaag
    10501 agctggattg ccagtagcgg taaataaatc tcaacccttg ccgttaatgc gccaggtgca
    10561 gatcgctgcg ggtggtttaa tattaatcgg cgttgtactg ggttataccg taaatagcgg
    10621 tttcttctta ttaagtggct ttgttggtgc cgggttactg tttgcaggaa tcagcggttt
    10681 ttgtggaatg gcaaggttgt tagataagat gccgtggaac caacgagctt gagaagcgac
    10741 gccggacgcg ccctagcagc gacatccggc ctcagtaatt agatcaggaa atcgtcgaga
    10801 gatttacctt ctgccagcgc ctgagcaatt ggcttcggtg tacggccctg accggtccag
    10861 gtcttagttt caccgttaac atcagtgaat ttatatttcg ccggacgcgg ctggcgtttt
    10921 ttaccagcac gtggtgcagc agcagagcta ttacccaata actcttccgg gttaattccg
    10981 tcagctttca tcagctccag ccaggtgcta attttttcct gacgctcagc cagttcacgc
    11041 tgctgctgtt cttcttcttc acgtctttct ttagtgacaa ccctgaattt ttcgagcatt
    11101 tcttcaagaa cgtcaatgga gaattcgcga gccatcgcac ggagggttcg aatgttattt
    11161 aaactttgta acattacgga cataataaag aaaacctttt aacgccaaaa caaaaaagta
    11221 tttcgtcgcg tagtgtaagc gatcacttat tatatttcca caaaatttaa tctatttaca
    11281 gtcctgcgcg agattatttc agctgttatt ttctgaaact ctcagaataa tttcaagatt
    11341 atatattagc cattttgatg agtaatttag gttattggtg aacagagtgg cgctctctgc
    11401 tcgctaactc cgcaagcctg ataaaaccga agtgaataaa aatgcaatat aacagaataa
    11461 aagaaaacac ccctcactgg tgctttctgc tgttaatgta cattttctgg catcaaaaat
    11521 gctgtaaatg acccggtcac gacaaacacc gaattctcta cgcttatagc gttacctcac
    11581 ccccaaactt tcattagtga aattaaaggc aaggtcatat ccaattgatt taattgaaaa
    11641 taaatcatat cggcatgtaa aaatccacaa tgtacaaaaa acgagctgtt acggaatatt
    11701 ttatctacaa aaactgacta aataaaagtt tttcactaat tgattagtca tagccagcga
    11761 tatacgctat gcgaaaatgc agatggcaat gagatccact gctttcatct ccattaacat
    11821 cccattacgc tttattaagg agcattagca tgttctcacc gcagtcacgc ttgcgtcatg
    11881 cagttgcaga tacgttcgcg atggttgttt actgttctgt cgtgaacatg tgtattgaag
    11941 ttttcctctc cggaatgagc ttcgaacagt ctttttattc cagattggta gcgattccgg
    12001 tgaacatctt aattgcatgg ccatacggta tgtaccgcga tctgtttatg cgcgcggcac
    12061 gcaaagttag cccgtcgggc tggataaaaa atctggctga tatcctggct tatgtgacgt
    12121 tccagtcacc ggtgtatgtg gcgatcttgt tagtggtggg cgcagactgg catcagatta
    12181 tggcggcggt cagttcaaac atcgttgttt cgatgttgat gggggcggtt tatggctact
    12241 tcctcgatta ttgccgccga ctgtttaaag tcagccgtta ccagcaggta aaagcctgac
    12301 tcttcttttc gcgactggca tcgccagtcg cgtctttaac tggcttcgcc aaataaacca
    12361 ttcaaataac gttcaagcgc aacacgcgaa ctgaagccat gcgggatacc gtaatgctcg
    12421 tgtcgctcgc ctgctaaata gagcggaaat tgctggtaat gatcgcaagt tttaatatcg
    12481 gaagcgggtt cagccagtgt tgaactgcgt tcttttaatg ttggatgaat taccagagcg
    12541 gtacgtccca ttcgcgcttc gcgattaaca tagacataat tttccccacg gcggtaacca
    12601 tacgctttct gggttaccac atcgacagta aaaccgactt tttcaagtac gcgcgccacc
    12661 tcgtctggtc gtaaatacat atttgatcct cgttatt
//
LOCUS       DAJVUJ010000125        12499 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.125, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000125 DAJVUJ010000000
VERSION     DAJVUJ010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12499
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.125"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..327
                     /locus_tag="OQQ48_003105"
     CDS             <1..327
                     /locus_tag="OQQ48_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003809694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCS1953790.1"
                     /translation="AQKFQGQYTGTDYVKILTESGGLPADMIAGGNKAKNAWGGAVTI
                     KVSSDKYSYVIESSNVPKKNCIDLVTSLRSSSMFTKINGNVTNKVDPSTVCNADKTTI
                     KLETNS"
     gene            372..857
                     /locus_tag="OQQ48_003106"
     CDS             372..857
                     /locus_tag="OQQ48_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HCS1953791.1"
                     /translation="MNRLFILTVIFSLLCSLPSAQASPQDKQYDRCFITAGKRYSIDP
                     GLLKIIARRESSLNPRAINHNRNDSGKIISTDYGIMQINSTHIPGLKKMGIIRSEKDL
                     LNNVCLNIQTGAWILARHFQRCGVNWECLGSYNAGFSKSNTHRRMKYARQIYSAYMQG
                     R"
     gene            873..1499
                     /locus_tag="OQQ48_003107"
     CDS             873..1499
                     /locus_tag="OQQ48_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCS1953792.1"
                     /translation="MLQIIPALLVAITVVIQCLAVQMSRYRLSRMNARPPHHGLALMS
                     GMLAAAAIILRLYQNDSAVGVLFMTIMTGCMTLMTLTDAASCRLPRPFTIMYLISGSA
                     FRLWQGDWLTGLCSAGFWFLLLLLFRQYTSYRRKTETIGLGDVFLIAGIAIWSPPGDI
                     PWIVATAAGGALLYLWCNRQRRITRELPFGPFLCASQYAFTFYPGGLW"
     gene            1517..2878
                     /gene="pilV"
                     /locus_tag="OQQ48_003108"
     CDS             1517..2878
                     /gene="pilV"
                     /locus_tag="OQQ48_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shufflon system plasmid conjugative transfer
                     pilus tip adhesin PilV"
                     /protein_id="HCS1953793.1"
                     /translation="MQKDNDKGFALLEILGGLVVISLLMPLFWSYIEDYLNEMRNQSA
                     AFHADAYNTAARTYIADNNARLHSGTLPATFTADELIRKGYLKGLNRSPFGQSYTTGI
                     RRNTSTGRLEALTCSTGGENIKDDALRSIASLLPGLGGFIGKNGTATGVFGGWTDKPG
                     DYGLSCNGGHIAIVMMGDDLQESDRLYRFQVPGRPELNQMNTAINMGGNNLNNAGNVN
                     GQSATLKGDVTSENGWLITKNDKGWKNITYGGGFTMTDSQWIRAVGGKGIITTGEIKG
                     GKVSGGTVRSDGRLSTGEYLQLDKTAVANTKCSPDGLVGRDSKGAILSCQSGVWVGFE
                     SYPVGSPIPWPSATPPQGYLLMNGQSFSCSRYPQLARAYPSCKLPDLRGVFIRGWDNG
                     KGLDGDRNRQLLSYQADQSGVYHERGGWLKGHHSGMPYWAQGSTTEMRPKNIAFNYIV
                     KAS"
     gene            3351..4172
                     /locus_tag="OQQ48_003109"
     CDS             3351..4172
                     /locus_tag="OQQ48_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HCS1953794.1"
                     /translation="MKTDSTGIAARMMLSLDRERICECLLSHRQLQSTPLQVRYPQGV
                     RDALGIMSEQLSLSVSDLTRILVEDALSEMFLPADNIVRRLLSRMEHIMQAHDISATT
                     MAALLAPWNIRPAVFREPDRLTDYLTGEILAALADWFYLSPEWLNGRVHYPLYRPGDW
                     PATQEIFCRIISARENMDIILWHGFPFAGTHSGEYCGVLLRQKKEINNTIIYPVLSLY
                     PVRMDIEKEGWFQMARKISPDIPVRAVTLTPAQAEYLITGKILPTALFRVPLSPW"
     gene            4274..5476
                     /locus_tag="OQQ48_003110"
     CDS             4274..5476
                     /locus_tag="OQQ48_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HCS1953795.1"
                     /translation="MMMKKHITRTLIASAVLFSFNSTAATSYFEARNDAMGGTGVASS
                     HYGVAPLANPALLTKSGAKDDFSLLLPSVGAQLSDPGNIADNADKISDDWKAFDRAVD
                     SHSGVPQTAARLKERLQDFRNTHASAQAGVSAVAALPGDTLAAALMLKTFGTVSVDGK
                     VSDADLNYLESIADSGSQDVDKNRLTSQAFARAALITDVGVALATELETAGQKWSLGF
                     TPKFQRVDLFNYNTLIKNYDSSAFKGDRYHNTQNGINADIGASMDLDDNWTLGLVAQN
                     LIPRSIETKEVHGVTETFRIRPQVTTGVSWHNDMFTTALDVDLTPASGFTSDSKRQFA
                     AVGAEFNAWKWAQLRTGYRQNLVSNNGSAFTAGIGLSPFDVIHIDVAGLVGTDNNYGA
                     IAQLQFTF"
     gene            5580..6038
                     /locus_tag="OQQ48_003111"
     CDS             5580..6038
                     /locus_tag="OQQ48_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238171.1"
                     /note="Members of this family include DotD of type IVB
                     secretion systems and TraH of plasmid conjugative plasmid
                     systems, both lipoproteins; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotD/TraH family lipoprotein"
                     /protein_id="HCS1953796.1"
                     /translation="MQKKRITLAGGVAMLCLSGCHLPPDNTAPARKYGAASEVAVTRH
                     AQYQLWEQGTYSAALPSRQPGGSLQAHSGRVSFDWEGDALELLSELARLRGQQFSYSG
                     VRLPLPVSLHVRDMTFADALRLTEAQTAWRAVLSQSAGRLHLSYMPTEKK"
     gene            6035..6871
                     /locus_tag="OQQ48_003112"
     CDS             6035..6871
                     /locus_tag="OQQ48_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000745049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HCS1953797.1"
                     /translation="MKKQVCLLLSGLLLAGCAGQHHRPPAGMAGNLPPPDLRAWLTPT
                     DNQDDDISDTRRQMLTDAGRTLGFRGGKAQRSWELIQALNARESTLNALYDFRPLISK
                     EGWLPPVIDEAQDVAHIQPDQIRTASRVWTILRPERFVSNPPGWRDWLLRGLSTTATP
                     GTEGRVVPEDRAQRRLWENALRQGWQEGRDNADLTLEANQKRLTRDYRGMMLYALLWR
                     QGMITRPDVTEQRQTVTGNGRKLITGDHVRRLKTHAEFTLQKSRWRPVVSTEGAPDEI
                     TR"
     gene            6855..8003
                     /gene="traJ"
                     /locus_tag="OQQ48_003113"
     CDS             6855..8003
                     /gene="traJ"
                     /locus_tag="OQQ48_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid transfer ATPase TraJ"
                     /protein_id="HCS1953798.1"
                     /translation="MKLPDEFGTFPFARFTAEEFRYFFAWCARHRVSDIDLTGGSPVS
                     VSRFGRRVRSSAHPLPSSIMMALSDELFGREVLPRVLSGHPVDRTIQVSGDASGRYGL
                     QRGQRVRLRCHLIQGTSATEEKVISVTMRVIPSDIPDILTMNIEPDLLDAMLRKTGLG
                     FICGETGSGKSTLAAALYRYIQTHFPDRKTVTYEDPVEYILGGENDLLPPHQAEIGRD
                     VESFAAGLRSAVRRNPDIIGIGEIRDYETADAAVQAGNTGHFCIGTMHTKSPGETFAR
                     LLGLFPPEIRDSMAAATLSLVQFILVQVLVRTNDGGRQAVREYIVITDELRDTLSRQS
                     HATWGHYIDEIIRKEKRRIRDQVLTMYQTGSIEASEAALFIPAGEFPK"
     gene            8000..8290
                     /gene="icmT"
                     /locus_tag="OQQ48_003114"
     CDS             8000..8290
                     /gene="icmT"
                     /locus_tag="OQQ48_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IcmT/TraK family protein"
                     /protein_id="HCS1953799.1"
                     /translation="MKREGFWRDVTPPLNIYGVPLPFTMIYLLLFPYPSKTTFWVCSG
                     ILLFFVLLGYSGWTVRTLCRRLYSLVQGKQASGRPWWYRHRTELPREWRGTE"
     gene            8354..12415
                     /locus_tag="OQQ48_003115"
     CDS             8354..12415
                     /locus_tag="OQQ48_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCS1953800.1"
                     /translation="MKSRYRTWLAVPPEETEAVKNAVPPLNGRKAVAWDPEKKLWYAR
                     AGTELSLLERWLPRPQELSMDAGDPVTEFAQVLENAGLVIQGLPQMDGAIHRVATRDD
                     KKGAKSGAYRAYLDGRPAGWYRDYRSADDSPTNWVFSGGEQHDPLARLHLRAFAQQQR
                     DDNARKLQQQYNKQAGYARSYINGLPQATAHEYLTRKGIRAAPGVRLNNKNELVIPFS
                     NGRGEIRSYQRIPVTGGKDARILKDSEKTGNWFTFGTPENGRPLLFAEGYATAASLHE
                     ATGLPVLMTVDASNMIAVAENARQIWTDSPFVFCADNDHQREINKGVFSATKAAEVTN
                     GEVIIPAFTEAEKAQGLTDFNDLDASRGRDNFQNAMNAQLKHIGIPTPNSDTADHREA
                     VVIDNLIFTPVKDEKPQMSPENRQSTAPETELATQDTPYDTHSDDTENQAYEDDYAAY
                     ASLQSVTAETLSPDKPLENIASAADTGTENTSVPTAGDQEDLNTDATGTDTAHNSDTA
                     ETGTLPEPAGMQSVQQATTEPTAEDKPHENVVSAADTREENASVATADTGEENASATT
                     ADNQSTQAEEHPRSEDTPETSLSDAAGTQHTTTTQSEEQVQVSMASADDWSAFAEDLN
                     ADTPGAPTVHNTDNAETNTVSEPAGMRPVQQVTTELTAEDKAQEKSVASAAETGAEHS
                     SDITDDNRNAPAEELNADATVAVHNTGTAETASPSEPAETQPVRQATAEPTAEDKLQE
                     ERLVSATETSTANEAARPETPSLFSRLRRFLTGGKSRTSEPSVIQEKKPTEAETATEE
                     VAPPSPPETSPEDTSSAEPDTIIFAPRRPDSPQRHNLDEILKNLTHKEFPDRTALYSV
                     DGEPAFLDRLYCLEMVDGASADDKKVLAALAVATNFYGGVIELTGSDAFKQKAMQLIV
                     EYDIKVRMKLPAQRAALEKIRKEKEMAHDAIVTHRPTPELNRQSPEETTMAQPAPPVS
                     PKAEAEQTAGSTAETPHPEAPVTAKAEAPRADRPEQPAAPSSAPEKAPVSAQPQQPDT
                     PEEEPPGKLSPGESVTAVLTNYGEKEYAPGKGRCFFVELTNRSGKREYWGNGLEELVK
                     NHQKGDPVTLTLQARETFRLSGETRDRIRNIWAITPVSNGITVNNDQPHAGQHTLEFP
                     MATFSQVMLQMQQHWPQIMQEVRSPKNPPNNLYIGEDRQPASAPQDQSMRLPLAGNAP
                     NELIPAIASADKDNRQLNLLLVHSADDHLQGVVRLNGTLYPALATPSADNRQLVINAL
                     TDNGLQFAGYGEAVNHDENNHQRPSPQIMQFHLKQQDSPLFAAIHKPEEQPDKLFRSL
                     GFEQTWKEWSDSQKAEDRQEKTLQQAQSHSPGR"
BASE COUNT         3163 a         3541 c         3233 g         2562 t
ORIGIN      
        1 gcccaaaaat tccaggggca gtatacaggt actgactacg tcaaaatcct gaccgaaagt
       61 ggtggcctgc ctgccgatat gatcgccggt ggtaataagg cgaaaaatgc ctggggtggt
      121 gcggtaacca tcaaagtcag ttcagataaa tactcctacg ttattgaatc cagcaatgtg
      181 ccgaagaaaa actgtattga tctggtcacc agcctgcgca gttcatccat gtttacaaaa
      241 atcaacggca atgtgacgaa taaagttgac ccgtctacag tatgtaacgc agataaaact
      301 actatcaaac tggaaaccaa cagttaatca ttcatcagcg cccgcccggg cgctttattt
      361 aaggtcactg tatgaacagg ttatttatcc tgacagtgat tttttcactg ttatgctcac
      421 ttccttccgc acaggcatcc ccacaggaca aacaatatga tcgttgtttt attaccgccg
      481 gaaaacgtta ttccattgat ccaggattac tcaaaattat tgcccgcagg gaaagcagtc
      541 tgaatccccg ggccattaat cataacagga atgacagtgg gaaaattatc agtaccgatt
      601 acgggataat gcagattaac tccacacata ttcccgggct gaaaaaaatg ggcattatcc
      661 ggagtgagaa agatctgttg aataatgtct gcctgaatat tcagaccgga gcatggatcc
      721 tcgcccggca ttttcagcga tgcggcgtaa actgggaatg cctcggcagt tataacgccg
      781 gcttcagtaa aagtaacact caccgccgga tgaaatacgc ccggcagatt tacagcgctt
      841 acatgcaggg acgttagtcc gggagaactg ccatgctcca gataatcccg gcactgctgg
      901 tcgccataac cgtggtaata cagtgtctgg ccgttcagat gagtcgctat cgactctccc
      961 ggatgaatgc ccgcccccct caccatgggc tggcgctgat gagcgggatg cttgcagctg
     1021 ccgccattat cctgcgtctt tatcagaatg acagcgctgt cggggtgctg tttatgacga
     1081 taatgaccgg atgtatgaca ctgatgaccc tgacggatgc tgcatcctgc cgtcttcccc
     1141 ggcccttcac catcatgtat ctgataagcg gcagcgcatt tcgtctctgg cagggagact
     1201 ggctgacagg cctgtgcagt gcagggttct ggttcctgct gttgcttctg ttcaggcaat
     1261 acacctctta ccgcaggaaa acagaaacca taggcctggg cgatgttttt ctgattgccg
     1321 gtattgccat ctggtccccg ccgggagaca tcccctggat tgtcgcaacg gcagcaggcg
     1381 gagccttact ttatctgtgg tgtaacagac aacgacgcat taccagagaa cttccctttg
     1441 ggccgtttct ctgcgcgtca cagtacgcat ttacttttta tcccggtggc ctttggtaat
     1501 accggaggga acgactatgc aaaaagacaa cgataaaggt tttgcacttc ttgaaattct
     1561 gggaggactg gttgtcatca gcctgctcat gccgctgttc tggtcttaca ttgaagacta
     1621 cctgaatgaa atgcgcaacc agagcgccgc atttcatgca gatgcctaca atactgcagc
     1681 caggacctac atcgccgata acaatgcccg cctgcatagc ggaacgcttc ctgccacctt
     1741 cacggcggat gagttaatcc ggaaaggata tctgaagggt cttaaccgct cgccctttgg
     1801 gcagagttat accaccggca tccgtcgtaa cacgagtacc ggtcgtctgg aggccctgac
     1861 ctgctccacc ggaggggaga atatcaaaga cgatgccctg cgcagtatcg ccagcctgct
     1921 ccccggtctg ggcggattta taggaaaaaa tggcacagca accggtgttt ttggtggatg
     1981 gacagataaa cccggcgact atggactcag ctgcaatggc gggcacatcg ccatcgtaat
     2041 gatgggtgat gacctgcagg aaagcgatcg cctgtatcgc tttcaggtac cgggacgacc
     2101 ggagctgaac cagatgaaca cggccatcaa tatgggaggg aacaacctga acaatgcagg
     2161 aaacgtcaat ggacaaagtg caacactgaa aggtgatgtt acaagcgaaa acggctggtt
     2221 aatcaccaaa aatgataaag gctggaagaa catcacctat ggtggcggtt ttacgatgac
     2281 agacagccag tggatacgcg cagtcggtgg taaaggaatt ataaccacag gggaaatcaa
     2341 aggaggaaaa gtcagtgggg ggacagtccg gtctgacggt cgcctttcca caggtgagta
     2401 tttacagctg gataaaacag ccgtagcaaa tacaaagtgt tcaccagatg ggcttgtagg
     2461 ccgcgacagc aaaggggcaa tactttcgtg ccaatccggt gtgtgggttg gctttgaatc
     2521 ctaccctgtg ggaagcccta tcccgtggcc atctgccact cctccacaag gctatctgtt
     2581 gatgaacggc cagtcgtttt cctgttcccg gtatcctcag cttgcccgtg cttatcctag
     2641 ctgtaagtta cccgatctgc gcggtgtttt tatccgtggc tgggataacg ggaagggact
     2701 tgatggtgat cggaacagac agctcctgag ttatcaggcc gatcaatcag gcgtctatca
     2761 cgaacgtggc ggatggctga aagggcatca tagtggtatg ccatactggg cacaggggag
     2821 taccacagaa atgcgtccca aaaacattgc attcaactac attgttaaag ccagctaggg
     2881 actcacatct gataggcata cacagagaaa acaccgttac tacaggcgta attttgtgtt
     2941 ggtctggaga tgatttgata tgacgctccc actggcacgg caaatgaaat aaatctccgg
     3001 gcatatcagc tggtcagcaa actcgatgcc aggcgtaaac agaccagcaa aatttcccct
     3061 tattttgttc cctaccctgc aactgtcggg tgcagaaacg ggctgtttgt cgtgacactg
     3121 cacgatttcg accttgaaac ccgtgcagaa acccggggac tggcaatatc tcatgccagc
     3181 atgctcagtc acctcctgtc cgaatccttt tcattcgccc ttaaaacaga gttacaaaca
     3241 ctcttgaaac acactcgatc ataacgatcg aaataactcg cttccgttta aaaaacactc
     3301 tctttagact caatttatga acaaaacacg ctgacccgca tggaggtgaa atgaaaactg
     3361 acagcacagg tattgccgcc cgtatgatgc tctccctgga cagggagcga atttgcgaat
     3421 gcctgctcag tcatcgccag ctgcagtcca cacccctgca ggttcgttat ccgcagggag
     3481 tcagggatgc ccttggtatc atgagcgaac agctttctct ttccgtctct gacctgacgc
     3541 gtatcctggt cgaagacgcg ttaagtgaaa tgtttcttcc tgcagataac atcgtccgcc
     3601 gtcttctcag caggatggag cacatcatgc aggcacatga tatcagcgca acaacgatgg
     3661 ctgcattact ggccccctgg aatatccgcc ctgccgtctt ccgggagccg gacagactca
     3721 ctgactatct gaccggggag attcttgcag cactggctga ctggttttat ctcagtccgg
     3781 aatggctgaa cggcagggtg cattatccac tgtatcgtcc gggagactgg ccagccacac
     3841 aggagatatt ctgcaggatt atttcagccc gcgagaatat ggacattatt ctctggcatg
     3901 gttttccgtt tgccggaacg cattccggag aatattgcgg cgttctgctc aggcagaaaa
     3961 aagaaattaa taacaccatt atttatccgg tactttcgtt atatccggtc agaatggata
     4021 tagaaaaaga aggctggttt cagatggccc gaaaaatatc tccggatatc cctgttcgcg
     4081 ccgtaacact cactccggca caggcagaat atctgataac aggaaaaata ttacccactg
     4141 cgttattcag ggttccgtta tccccctggt aataatactg agcaataaac cggaataata
     4201 caccaactgt ttttaatggc acagccgtgc cctctgttgt tatacccaaa atacctcaat
     4261 acaggaaact gaaatgatga tgaaaaagca tattacccgt actctcatcg cgtctgccgt
     4321 tttattttcc tttaattcga ctgcagcaac gtcatatttt gaagcccgca atgatgcgat
     4381 gggaggaacg ggggttgcct cctctcacta tggtgtggcc ccactggcaa acccggcact
     4441 gctgacaaaa tccggtgcaa aagatgactt cagtcttctg cttccgtccg tgggtgcgca
     4501 gctgtccgat ccgggtaata tcgccgacaa cgccgacaaa atcagcgatg actggaaagc
     4561 cttcgaccgt gccgtcgaca gccactccgg ggtaccgcag acagcggccc gcctgaaaga
     4621 gagactgcag gacttccgga acacccatgc cagcgcacag gccggggtat ctgctgtggc
     4681 ggcccttccc ggtgataccc tcgctgctgc actgatgctg aaaacgttcg ggactgtcag
     4741 cgtcgacggt aaagtcagcg atgccgacct gaactatctg gaaagcattg cagacagtgg
     4801 cagccaagat gtggataaaa acagactgac ctcacaggct tttgcccgtg ccgcactgat
     4861 caccgatgtg ggcgttgcgc tggccactga actggaaacg gccggacaaa aatggtcact
     4921 gggcttcaca ccaaaattcc agcgtgttga cctgtttaac tacaatacgc tgatcaaaaa
     4981 ctatgacagc agcgcattca aaggtgaccg ttaccacaac acccaaaacg gtattaacgc
     5041 tgatatcggt gccagcatgg acctggatga caactggacg ctgggtctgg tcgcacagaa
     5101 cctgatccca cgcagcattg agaccaaaga ggtacatggt gtcactgaaa ccttcaggat
     5161 ccgtccgcag gtaacgaccg gcgtatcatg gcacaacgac atgttcacca cggcactgga
     5221 tgtggatctg accccggcca gcggcttcac atcagacagc aaacgtcagt ttgccgccgt
     5281 tggtgctgag tttaatgcct ggaaatgggc acagttacgt accggttacc gccagaatct
     5341 ggtcagcaac aacggcagtg cattcactgc cgggattggg ctctcaccgt ttgatgtgat
     5401 tcacattgat gtcgccggcc tcgtgggcac cgacaacaac tacggtgcca ttgctcagtt
     5461 acagttcacc ttctgattat cacgccccgg gacgcccggg gcttctgacc tgaatctgct
     5521 cgtttccgct cttctggcca ctcaccgcca gtcctgttca ttattttccg gataaaacca
     5581 tgcaaaaaaa acgtatcacc cttgcagggg gagttgccat gctctgtctg agcggctgtc
     5641 atcttccccc ggataatacc gcaccggcca ggaagtacgg tgcagcttct gaagtcgctg
     5701 tcacacgaca tgcacagtat cagttgtggg agcagggcac gtattctgca gccctgcctt
     5761 cccgccagcc cggaggcagc ctgcaggctc acagcgggcg ggtttccttt gactgggaag
     5821 gcgatgctct ggagctcctg agcgagctgg cccgtctgcg cggacaacag ttcagttaca
     5881 gcggcgtgcg tcttcccctg cccgtctcac tccatgtacg cgatatgacc ttcgctgatg
     5941 cccttcgtct cacagaggcc cagactgcct ggcgggcagt cctctcacag tcagccgggc
     6001 gtttacatct ttcctatatg ccaacggaga aaaaatgaaa aaacaggtct gtctcctgct
     6061 gtccgggctc ctgctggcag gctgtgccgg acagcaccat cgcccccctg ccggtatggc
     6121 aggcaattta ccaccgccgg atctccgggc atggctgacg cccactgaca accaggatga
     6181 cgacatttca gacacccgtc gccagatgct cacggacgcc ggacgaacgc tgggattccg
     6241 tggcggaaaa gcacagcgct catgggaact gattcaggca ctgaatgcac gggaaagtac
     6301 actgaatgcc ctgtatgact tccgtcccct tatcagcaag gaaggctggc taccccctgt
     6361 tattgatgaa gcgcaggatg ttgctcacat tcagcctgac cagataagaa ccgcgtcccg
     6421 tgtctggacc atcctgcgcc ccgaacgttt tgtcagtaac ccgccgggct ggcgtgactg
     6481 gcttctgcgg gggctttcca ctaccgccac cccgggaact gagggccgtg ttgtaccgga
     6541 agaccgcgca cagcgcagac tctgggaaaa cgcactccgc cagggctggc aggaaggccg
     6601 tgacaacgct gacctcaccc tggaagccaa ccagaaacgc ctgacccggg attaccgggg
     6661 aatgatgctt tatgccctgc tctggcgtca gggcatgatc acacgcccgg atgtgactga
     6721 acagcggcag accgtcaccg gtaacggcag gaaactcatc accggagacc acgtccgccg
     6781 tctgaagaca cacgcggaat tcaccctgca gaaatcacgc tggcgtccgg tcgtcagcac
     6841 ggaaggagcc cccgatgaaa ttacccgatg agttcggcac attccctttc gcccgtttca
     6901 cggccgaaga gtttcgctat ttttttgcct ggtgtgcccg acaccgcgtc agtgatatcg
     6961 atctgacagg cggctcaccg gtatctgtca gtcgctttgg acggcgggta cgttcgtccg
     7021 cacatcctct gccgtcctcc atcatgatgg cgctgagtga tgaattgttt ggacgggaag
     7081 tccttccccg ggtgctgtcc ggtcatcccg tggaccgtac cattcaggtc agcggtgatg
     7141 cctccggtcg ctacggtctg caacgagggc agcgtgttcg cctgcgctgt catctgattc
     7201 agggaacatc agcaacagaa gaaaaagtaa tctccgtcac catgcgggtg atcccgtccg
     7261 atattccgga catcctcacc atgaatattg aaccggacct gctggatgcc atgctccgga
     7321 aaaccggtct gggtttcatc tgcggagaga ccggctccgg taaatccaca ctggcagcgg
     7381 cactgtaccg ctacattcag acgcatttcc ccgaccgcaa aacggtgacc tatgaagatc
     7441 cggtggaata cattctgggg ggagagaatg accttctgcc accccatcag gccgaaatcg
     7501 ggcgtgatgt ggaaagtttt gcggcaggac ttcgctctgc cgttcgccgt aacccggaca
     7561 tcatcggtat cggtgaaatc agggattatg aaacggcaga tgcagccgtc caggcaggta
     7621 acaccggtca cttctgcatc ggcaccatgc acaccaaatc acccggtgaa acctttgccc
     7681 ggctgctggg gttatttcca ccggaaatca gggattcaat ggcagccgcc accctttccc
     7741 tggtgcagtt cattctggtg caggtcctgg tcagaacaaa tgacggtggc agacaggccg
     7801 tccgggaata catcgtcata actgacgaac tgcgtgatac gctctcccgg cagtcccatg
     7861 cgacatgggg gcattacatt gatgaaatca ttcgcaagga aaaacggcgg atacgggatc
     7921 aggttctgac catgtatcag acgggcagca ttgaggcatc agaagctgcg ctcttcatcc
     7981 cggccggaga attccccaaa tgaaacgcga aggcttctgg cgggatgtca ctccgccact
     8041 gaatatttat ggtgtcccgt tgccgtttac catgatttac ctgctgctgt ttccgtatcc
     8101 gtcaaaaaca acattctggg tgtgcagcgg cattctgctg ttttttgtgc tcctggggta
     8161 ctcaggatgg actgtccgga cgctctgccg gcgtctgtac agcctggtgc agggtaaaca
     8221 ggcgtcaggc cgtccctggt ggtaccggca tcgcacagaa ttaccccgtg aatggcgcgg
     8281 aacggaataa ccctcttttg ttcatcatcc cggcgtatca caacgccctc atctttaatc
     8341 cccggagaac gacatgaagt ccagatatcg aacctggctt gctgtcccgc ctgaggagac
     8401 agaagccgta aaaaatgcag ttcccccgtt aaatggccgc aaagctgtcg catgggatcc
     8461 agaaaaaaag ctctggtatg cccgggcggg taccgaactt tcactgctgg agcgctggtt
     8521 accccggcca caggaactgt cgatggatgc cggcgatccg gtcactgaat ttgctcaggt
     8581 cctggaaaac gccgggctgg ttattcaggg attgccacag atggacggag ccatacacag
     8641 agtggccacc cgggatgaca aaaaaggggc caaaagtggc gcatacaggg cctatcttga
     8701 tggtcgtccc gccggctggt accgcgacta ccggagcgcc gatgacagtc ccacaaactg
     8761 ggtattttcc ggcggagaac aacacgatcc tctggccaga ctgcatctgc gggcattcgc
     8821 tcagcaacag cgcgatgata acgctcgtaa actgcagcaa cagtataaca aacaggccgg
     8881 ctatgcccgc tcctatatta acgggttgcc acaggcaaca gcacatgaat acctgacgcg
     8941 caagggcatc cgggccgcgc ctggcgtgcg gctgaacaat aaaaatgagc tggttattcc
     9001 cttcagcaac ggccggggag aaatacgttc gtaccagcgt attccggtca ccggcgggaa
     9061 ggatgcccgc attctgaaag actccgagaa aaccggcaac tggttcacct tcggaacccc
     9121 ggaaaatggc cgccccctgt tatttgcgga aggttacgct actgccgcct ccctgcacga
     9181 agcaaccggc cttccggttc tgatgacagt tgatgccagc aatatgattg ccgtggcaga
     9241 aaatgcccgc caaatctgga ctgacagccc ctttgtcttc tgtgccgaca acgatcatca
     9301 acgggaaatt aacaaagggg tttttagtgc aacaaaggcc gcagaagtca cgaatggcga
     9361 agtcattatt ccggccttta cggaggcgga aaaagcacag ggactgaccg atttcaatga
     9421 cctggacgcc agccggggac gggacaactt tcagaatgcc atgaatgcac agttaaaaca
     9481 tatcggtatc cccacaccca acagtgacac tgccgaccat cgtgaggctg tcgtgatcga
     9541 caacctgata ttcacaccgg taaaagatga gaagccacaa atgtcccctg aaaacagaca
     9601 gagcacagcc ccggaaacgg agctggcgac acaggatacg ccttatgaca cccactccga
     9661 cgatacagaa aaccaggcat atgaagatga ctatgccgcc tatgcatccc tgcaatcagt
     9721 aactgcggag acgttatccc cggataaacc actggagaat attgcgtctg ccgctgatac
     9781 cggcacagag aatacctctg tgcctaccgc tggcgaccag gaagacctca atacagacgc
     9841 cacaggtaca gacaccgctc acaacagcga cacagcagaa acaggaacgc ttcctgaacc
     9901 tgccgggatg cagtccgtgc agcaggcaac cactgaaccg accgcagaag ataaaccaca
     9961 cgagaacgtt gtttccgctg ccgataccag agaagaaaat gcctctgtgg ctaccgctga
    10021 taccggtgaa gaaaatgcct ctgccaccac cgctgacaac cagagcacgc aggcagaaga
    10081 acatccccgg agtgaagaca caccggaaac ctccctctcc gacgcggccg ggacacagca
    10141 caccaccaca acgcagtctg aagaacaagt gcaggtttcc atggccagcg ctgacgactg
    10201 gagcgcattt gcggaagatc tcaacgctga cactccgggg gcccccactg ttcacaacac
    10261 cgacaacgcg gagacaaaca cagtttctga accggccggg atgcggccag ttcagcaggt
    10321 gaccactgaa ctgacggcag aagataaagc acaggaaaag agcgttgcgt ctgccgctga
    10381 gaccggcgca gagcactcct ctgacatcac cgatgacaac aggaacgcac ccgctgagga
    10441 gctcaatgcc gatgccacgg ttgctgttca caacaccggc acagcagaaa cagcctcacc
    10501 ttctgaacct gcagaaactc agccagtccg gcaggccacc gctgaaccga cggctgaaga
    10561 taaactgcag gaagagcgtc ttgtgtccgc cactgagacc agcacagcga atgaagcagc
    10621 ccgccctgaa acgccatcgc ttttcagccg tctgcgccgt tttctgaccg gtgggaaatc
    10681 acgcacctca gaaccttccg ttattcagga gaaaaagccc acggaggctg aaacagcaac
    10741 agaggaggtc gcaccaccat caccaccgga aaccagcccg gaagacacat cgtcagcgga
    10801 accggacacg attatctttg caccccgccg tcccgacagt ccgcagcggc acaacctgga
    10861 cgagatcctt aaaaatctta cacacaaaga gtttcccgac aggactgcgc tctacagcgt
    10921 tgacggtgaa cccgccttcc tggacagact ctactgtctc gaaatggtgg atggcgccag
    10981 cgcggatgac aaaaaggtcc tggctgccct tgctgtggcc accaacttct atggcggcgt
    11041 tatcgagctg accggcagtg atgcctttaa gcaaaaagcc atgcagctga ttgtcgaata
    11101 cgacatcaag gttcgcatga aactcccggc ccagcgggca gcactggaaa aaatccgcaa
    11161 ggaaaaagag atggcgcatg atgccattgt cacccacaga cccacgccgg aactcaaccg
    11221 ccagtcaccg gaagagacaa caatggccca accggctccc cctgtatcac cgaaggcaga
    11281 ggctgaacaa acagcaggca gtacggctga aacaccgcat ccggaagcgc cggtcacggc
    11341 aaaagctgaa gccccccggg ccgacagacc ggaacaaccc gccgccccgt cgtctgcgcc
    11401 agaaaaagcc cctgtcagtg cacagccgca gcaacccgac acaccggagg aagagccgcc
    11461 cggcaagctc agccccggtg aaagtgtgac tgccgtcctg acaaactacg gagaaaaaga
    11521 gtatgcgccc ggaaaagggc gctgcttttt tgtggaactc accaaccgca gtggcaaacg
    11581 ggaatactgg ggaaacggcc tggaagaact ggtcaaaaac catcagaagg gtgaccccgt
    11641 cacactgaca ctgcaggcga gagaaacatt ccggctgtcc ggtgaaacgc gggatcgtat
    11701 ccgtaatatc tgggccataa ccccggtcag taacggcatt accgttaata atgaccaacc
    11761 tcatgcgggt cagcataccc tggagtttcc gatggcaaca ttcagccagg tgatgttaca
    11821 gatgcaacaa cactggccac agataatgca ggaagttcgc tcccccaaaa atcctcccaa
    11881 taatctgtac attggtgaag acaggcaacc tgcatcagca cctcaggatc aatcaatgcg
    11941 actccccctc gctggtaatg cccccaatga actgatacca gccatcgcct ctgccgataa
    12001 ggacaatcgt cagttgaatc ttctgctggt ccacagcgca gatgatcatc tgcagggcgt
    12061 ggtccgcctg aacggcaccc tttacccggc actggccacc ccgtcagctg acaaccgtca
    12121 gctggttatc aatgccctga cggataacgg tctgcagttt gccggttacg gtgaagctgt
    12181 taaccatgat gagaataatc atcagcgtcc gtccccacaa ataatgcagt ttcacctgaa
    12241 gcaacaggac tccccgttgt ttgccgccat ccacaaaccg gaagagcaac cggacaagct
    12301 tttccgctct ctgggttttg agcagacctg gaaagagtgg tctgacagcc aaaaggcgga
    12361 ggacaggcag gaaaaaactc tgcaacaggc acaatcccac agccccggcc gatgagccgg
    12421 taaggacgac catgaacatg aaaaaaacgc tgctggtcat actgaccggc atcaccctgg
    12481 catcaccggt ggcaggtgc
//
LOCUS       DAJVUJ010000126        12464 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.126, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000126 DAJVUJ010000000
VERSION     DAJVUJ010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12464
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.126"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(94..1185)
                     /gene="ychF"
                     /locus_tag="OQQ48_003116"
     CDS             complement(94..1185)
                     /gene="ychF"
                     /locus_tag="OQQ48_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCS1953801.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(1302..1886)
                     /gene="pth"
                     /locus_tag="OQQ48_003117"
     CDS             complement(1302..1886)
                     /gene="pth"
                     /locus_tag="OQQ48_003117"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCS1953802.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            2164..2442
                     /gene="ychH"
                     /locus_tag="OQQ48_003118"
     CDS             2164..2442
                     /gene="ychH"
                     /locus_tag="OQQ48_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HCS1953803.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(2497..4176)
                     /gene="dauA"
                     /locus_tag="OQQ48_003119"
     CDS             complement(2497..4176)
                     /gene="dauA"
                     /locus_tag="OQQ48_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HCS1953804.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVIIYPVSQQFGL
                     AGLLVAALLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPISGRLAFFPNRRAAMADL"
     gene            complement(4301..5248)
                     /gene="prs"
                     /locus_tag="OQQ48_003120"
     CDS             complement(4301..5248)
                     /gene="prs"
                     /locus_tag="OQQ48_003120"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCS1953805.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(5399..6250)
                     /gene="ispE"
                     /locus_tag="OQQ48_003121"
     CDS             complement(5399..6250)
                     /gene="ispE"
                     /locus_tag="OQQ48_003121"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCS1953806.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(6250..6873)
                     /gene="lolB"
                     /locus_tag="OQQ48_003122"
     CDS             complement(6250..6873)
                     /gene="lolB"
                     /locus_tag="OQQ48_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HCS1953807.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTHPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            7087..8343
                     /gene="hemA"
                     /locus_tag="OQQ48_003123"
     CDS             7087..8343
                     /gene="hemA"
                     /locus_tag="OQQ48_003123"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HCS1953808.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            8385..9467
                     /gene="prfA"
                     /locus_tag="OQQ48_003124"
     CDS             8385..9467
                     /gene="prfA"
                     /locus_tag="OQQ48_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCS1953809.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELRKAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            9467..10300
                     /gene="prmC"
                     /locus_tag="OQQ48_003125"
     CDS             9467..10300
                     /gene="prmC"
                     /locus_tag="OQQ48_003125"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCS1953810.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGGREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            10297..10689
                     /locus_tag="OQQ48_003126"
     CDS             10297..10689
                     /locus_tag="OQQ48_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HCS1953811.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            10693..11502
                     /locus_tag="OQQ48_003127"
     CDS             10693..11502
                     /locus_tag="OQQ48_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HCS1953812.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDPVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            11538..12392
                     /gene="kdsA"
                     /locus_tag="OQQ48_003128"
     CDS             11538..12392
                     /gene="kdsA"
                     /locus_tag="OQQ48_003128"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HCS1953813.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
BASE COUNT         3110 a         3158 c         3240 g         2956 t
ORIGIN      
        1 cggtgaaatg aaaaatccac gcaattgcgt ggattttata tacttttgcg ctcttcatga
       61 gatttagcga aacctcatga gacaataaat taattagacg ttgaaaagga agttcatcac
      121 atcgccatct ttcacgatgt aatctttacc ttctgcacgc attttgcctg cttctttcgc
      181 gccttgttca cctttgtaag tgatgaaatc ttcaaacgag atggtttgtg cacggataaa
      241 gcctttttca aaatcagtat ggattttgcc cgctgcctgc ggcgcggttg ctccaaccgg
      301 aatggtccat gcacgcactt ctttcacccc agcggtgaag taagtttgca ggttcagcag
      361 cttataaccg gcacggatca cacggttcag gcccggctct tccagcccaa gctcctgcat
      421 aaactcgtca cgttcttcgt cgtccagttc ggcaatgtct gcttcaacag cagcacaaac
      481 cggaaccaca acagaacctt ctttcgccgc gatttcacgc acctggtcaa gatatgggtt
      541 gttttcaaaa ccgtcttcgt tgacgttggc gatgtacatt gttggtttca gcgtcaggaa
      601 gctcaggtaa cgaatagccg ctttctcttc agcgcttaaa tccagcgcgc gcagcatacc
      661 tgcattttcc aactggggca ggcatttttc caggaccgcc agctcagctt tcgcgtcttt
      721 atcgccacct ttggctttct tctgtacgcg atgaatcgca cgttcgcagg tgtcgaggtc
      781 tgccagcgcc agttcggtgt tgataacttc aatatcgtca gccgggttaa ctttgccgga
      841 aacgtgaatg atgttgtcat tttcaaagca gcgaacaacg tgaccgatcg cttcggtttc
      901 acggatgttg gtcaggaact ggttacccag accttcgcct ttcgatgcgc cttttaccag
      961 accggcgata tcgacaaatt ccatggtcgt gggaagcgta cgctgcggtt ttacgatttc
     1021 agccagttga tccaggcgag gatcaggcat tggtacgacg cctgtgttcg gctcaatggt
     1081 gcagaatgga aagttggccg cttcaatacc ggctttggtc agcgcgttga acagggtaga
     1141 tttcccgacg ttgggcaaac cgacgatacc gcatttgaat cccatgattt aaatcacctt
     1201 aatatcttaa taatcaacct gttattgcta acagattgca gaaatggaaa taactttgcc
     1261 tattatacac ggcactcggc aaaaatgccg cagacaacga cttattgcgc tttaaaggcg
     1321 tgcaatcggt tcgttgcttt ggtcaagcca tctgtaaacc acatttcagt acaacgcgcc
     1381 gcttcgtcaa tggcttcatc aattaacttc tgttcactaa caggcggttt gcctaacaca
     1441 aaaccgacaa ctttattttt atcgcccgga tgaccgattc cgatgcgtaa acggtgaaag
     1501 ttagggttat tacccaattt actgatgatg tctttcagtc cattgtgacc accatggcca
     1561 ccgcccaatt taaatttggc gacgccagga ggcagatcca gttcgtcgtg ggccaccaga
     1621 atttcgtccg gattaatgcg gaaaaaactg gccatcgccg caacggcttt gccgctgaga
     1681 ttcataaatg tagtcgggac taacaggcgg acatcttcgc ctccaagagt gactcgcgaa
     1741 gtataaccaa agaatttagc ctcttcgcgc agcggagcgc gcaaacgctc tgccagtaag
     1801 tcaacgaacc aggcaccagc attatgtcgc gttgcggcgt attcagcacc ggggttcgcc
     1861 aggccgacaa tcaatttaat cgtcacgttt ttttgtcctg agtgtgtaca taactggcgc
     1921 gtagtttact ggttgcgacc ccgcttgaca aaaaactgcg tatcaaatgc agataacgta
     1981 ataattgcct gagtgggcta ttagaaagtc aaggtgttca ggcgtttatt tgtaaagttt
     2041 tgttgaaata agggttgtaa ttgtgatcac gcccgcacat aacccacagg gtgttgtcta
     2101 tactttacac ataaggaaga ggggtattcc ctgttacaac ccagaaagtt ccggaggtga
     2161 catatgaaac gcaaaaacgc ttcgttactc ggtaacgtgc tcatggggtt gggtctggtg
     2221 gtaatggtgg tcggcgtggg gtattcaatc ctcaaccagt taccacagtt taatatgccc
     2281 cagtatttcg cacatggtgc agtgctaagt attttcgtcg gtgccattct ctggctggcg
     2341 ggtgcccgtg ttggcgggca tgaacaggtg tgcgaccgtt actggtgggt tcgccactat
     2401 gacaaacgtt gccgccgtag cgataatcgc cgtcatagct aacaaataat gcagtttgct
     2461 gaccagtcag tttgcgctga ctggtcaatc tcgtacttat aaatccgcca tcgccgcgcg
     2521 acgattcggg aagaacgcga ggcgtcccga gattggttga atgcctgcgc gagccatagt
     2581 gcgcagtggc tggaattcca cgttgcacac gcgcagttca catccttcgg gcagacgctt
     2641 cacaaaacgc tggaacgcat caagaccacc agcatcaagt accggaacgg catcccactt
     2701 cagaatcaca atccgtttgc cttcaagacg tgactccagg tccgtgaaca agccttcagc
     2761 agcagcaaaa aacagcgggc caataacgcg cagtaccaga acatcgtctg gaacatctac
     2821 gactaccggt gccaggcgag tcatacgtgc gatacgacgc ataaacagca gcgatgccag
     2881 cacgatcccc acgctgatgg caataaccat atcaaacagc acggtcagcg acatgcacag
     2941 cagcatgaca atgatgtcat ctttcggtgc atgacgcagc aagtcgacca ctttatgcgc
     3001 ttcactcatg ttccacgcca ccatcaacag cagggctgcc atagcggaaa gcggcagcca
     3061 ggagagcagc ggtgccagta ccagcagggc aagaataacc agaatagagt ggatcaccgc
     3121 tgagataggg gaagttgccc cggcacggac gttagcggca gaacgcgcga tggcagctgt
     3181 agcggtaata ccaccaaaga atggagcgat aatattcccc agtccctgtc caaccagttc
     3241 gctgttcgcc ttgtgtttcg tcccggtcat accatccagt accacggcgc agagcagaga
     3301 ttcgattgcg ccgagcattg ccattgagaa tgccgcaggc agcagtgtgc gaatagaatc
     3361 ccaggttagc gtgaattctg aattaggcag atcccacggc agcaccagtt gcggcagcag
     3421 ttgcggaata ccgttaccct gagaaccatc ggccaggacg tagtggaatt gcgaaccgat
     3481 ggtagcaaca tgtccgccga gcaggttaac aatccccatc accgcgcaac cagccagcaa
     3541 ggccggaagg tgaccgggta aacgaatgcc cagacgcggc caaaaaacaa gaatacctag
     3601 cgtcacaatg ccaatggcag catcacccac attaatggtc ggcagcgcca taaataatgc
     3661 gccgactttt tgtagataat gttccgggac atgggccatt tgcagaccga gaaaatcttt
     3721 aatctgcatg gtaccgatgg tgatcccgat acccgaggtg aaacctaagg tgacggaaac
     3781 cggaatatac tcaatcaggc gaccaaagcg tgccagaccc atcagaatca aaaagatccc
     3841 cgacagcaag gccgcaacca gcagtcctgc cagtccaaac tgttgcgaca cgggatagat
     3901 aattaccaca aatgccgcag tcggaccgga aacgctaaag cgtgacccac ccgtcagagc
     3961 aatgacaatc cccgcaacag ctgcggtata taaaccgtac tggggtgcca caccactacc
     4021 aatagccaac gccatcgcca gcgggatagc aataatcccg acggttatcc cggcaatcag
     4081 gtcacgggta aaccgtgcgg cagtatattt ttctttccag caagcgtcga tcagagcgcg
     4141 gaaaggcatc acatgtgagg aaaatatttt gttcacaata atgtttcatc cgtgagcgca
     4201 tcatctgtca actaaatggc aggtgaagga ggcataggtc atacaaatgg atattacaga
     4261 caaaaaaacc cgccgcagcg ggtctttgag ccgggttcga ttagtgttcg aacatggcag
     4321 agatcgattc ttcgttgctg atacgacgaa tcgcttcggc cagcatacct gacagggtca
     4381 gagtacgcac gttcggcagt gatttgattt catcgctcag cggaatggta tcgcagacaa
     4441 cgacttcatc aattacagag ttacgcaggt tgttcgccgc gttgccagag aagatcgggt
     4501 gagtcgcgta cgcaaataca cgtttagcac cacgttcttt cagagcttca gcagctttac
     4561 acagcgtacc gccagtgtcg atcatatcat cgaccagtac gcagtcacga cctgcaacgt
     4621 caccgatgat atgcatcacc tgggaaacgt tcgcacgcgg acgacgtttg tcgatgattg
     4681 ccatatcggt atcgttcagc agcttagcga tagcgcgggc acgcacaacg ccgccgatgt
     4741 ccggagaaac cacaattggg ttatccagat tcagctgcag catgtcttcc agcaggatcg
     4801 ggctaccaaa tacgttatca accggaacgt cgaagaaacc ctgaatctgt tcagcgtgca
     4861 gatccactgt cagcacacgg tcaacaccga cgctggagag gaagtctgca accactttcg
     4921 cagtgattgg tacacgagcg gaacggacgc gacggtcctg gcgcgcatag ccaaagtagg
     4981 ggataacagc ggtgatacga cctgcggaag cacgacgcag ggcatcaacc ataacgacta
     5041 attccatcag gttgtcgtta gtaggggcac aagtggactg gatgatgaaa atatcaccac
     5101 cgcgtacatt ttcattaatt tgtacgctga cttcgccatc gctaaagcga cctacagcgg
     5161 cgtcgccgag tgaagtgtac aggcggttgg caatacgttg tgctagttcc ggggtggcgt
     5221 taccagcaaa aagcttcata tcaggcacga gaagaacctc aggcatgcgt ccattggtgg
     5281 aaagaatctg ccgaaaactg tgcgggccag gcatgatcct ttccaggcag tgtattaaag
     5341 agcgcgatgc aacgtctgga acaaggtgac gttgtcaccg aaactcagct tgcccggctt
     5401 aaagcatggc tctgtgcaat ggggaaagat taacgccttt cgccacaaag ccattgagcc
     5461 attccggggc ttgctctagc acctggcggg cttcagactc tgtatcaaat tcagcaaaga
     5521 cacaggcccc tgtcccagtc aggcgcgacg gggcgtattc taacagccag gaaagcaccg
     5581 catcaacctc gcgaaaacgt tttcttgcga taacctcgca atcattgctg aattcacatt
     5641 ttagcaacgt ttctattgac ctttttggcg tattgcgcgg gagttcagga tctttaaaaa
     5701 tcaccggagt cggaatactt acaccagggt gcgccaccag ataccacttc tctggcggat
     5761 ccaccggcgt tagtatttca ccaacgcctt cggcaaacgc ggcatgcccc cgaacaaaga
     5821 caggaacatc tgcgcccagc gtcagcccca tttccgccag ctcatccatg cttagcccgc
     5881 attgccagag atgatttaat gccaccagga ccgtcgcggc attggatgaa ccaccgccga
     5941 gaccgccgcc catcggcaaa cgcttgtcaa tgctgatatt cgcaccgctt cccgtcggaa
     6001 gacgcccgct gtctgccgca gttttcatca acaatcgcgc tgcgcgaacg atcaggttat
     6061 cttcatgttc cacgccttca acgggcgtta acagacgaat atccccatcg tcacgaagct
     6121 caatgctgat ggtgtcgccg taatcaagaa actgaaacag cgtttgcagc gtgtggtaac
     6181 catccgcacg ctgaccggta atgtataaaa acagattaag ttttgccgga gagggccact
     6241 gtgtccgcat tatttcacta tccagttatc catttttaac ttgatgcgtt gaccaccgtc
     6301 ggtgagttcc atattggctg gcatcgcagg atgcgttttg gtgtcataac caccataaac
     6361 aaccttccag tttttgccat tctggctgta ggtaatttcg ctcaggcggt actggtcgtc
     6421 cagtttgtag tcggttgcat cacccggtaa acctaaaatc cactggcgca agctgttgag
     6481 cggaattggc attccggtca atttgccaat catctcttcg gcgtcatcgg cggtataacg
     6541 ctgaccttta ttgtcgacta actgcacgtt acccggttga gcattcagct ccagttccgt
     6601 gctgcccaat gggttagtga gcagcagacg gtagcgatcc tggccggttt gctgccagaa
     6661 aaagcgggcg tacacttttt gttggtcaga aatataagcg aacgcgccgc gagtctgata
     6721 ctgattaaga ttgcgcacgt cttgctgatg ctgacgccat tgtggcgaat ccgggctttt
     6781 gccaggacct ttgggcgtgg taacggaaca ggcagtgagc acaagagcag ccagcggtag
     6841 caggcggata agacgaaaat cgggcagggg catagtgatg acaagtcctt gagatacgtt
     6901 gcagttataa cccttaatgc tagcgttacc gtccgctatc gtctatgttc aagttgtctt
     6961 aattgccaga atctaacggc tttcggcaat tactccaaaa gggggcgctc tcttttattg
     7021 atcttacgca tcctgtatga tgcaagcaga ctaactctat caacgttggt attatttccc
     7081 gcagacatga cccttttagc actcggtatc aaccataaaa cggcacctgt atcgctgcga
     7141 gaacgtgtat cgttttcgcc ggataagctc gatcaggcgc ttgacagcct gcttgcgcag
     7201 ccgatggtgc agggcggcgt ggtgctgtcg acgtgcaacc gcacggaact ttatcttagc
     7261 gttgaagagc aggataacct gcaagaggcg ttaatccgct ggctttgcga ttatcacaat
     7321 cttaatgaag aagatctgcg taaaagcctc tactggcatc aggataacga cgcggttagc
     7381 catttaatgc gtgttgccag cggcctggat tcactggttc ttggggagcc gcagatcctc
     7441 ggtcaggtta aaaaagcgtt tgccgattcg caaaaaggtc atatgaaggc cagcgaactg
     7501 gaacgcatgt tccagaaatc tttctctgta gcgaaacgcg ttcgcactga aacagatatc
     7561 ggtgccagtg ctgtgtctgt cgcttttgcg gcttgtacgc tggcgcggca gatctttgaa
     7621 tcgctctcta cggtcacagt gttgctggta ggcgcgggcg aaaccatcga gctggtagcg
     7681 cgtcatctgc gcgaacataa agtacagaag atgattatcg ccaaccgcac tcgcgaacgt
     7741 gcccaaattc tggcagatga agttggcgcg gaagtgattg ccctgagtga gatcgacgaa
     7801 cgtctgcgcg aagccgatat catcatcagt tccaccgcca gcccgttacc gattatcggg
     7861 aaaggcatgg tggagcgcgc attaaaaagt cgtcgtaacc aaccaatgct gttggtggat
     7921 attgccgttc cgcgcgatgt tgagccggaa gttggcaaac tggcgaatgc ttatctttat
     7981 agtgtggacg atctgcaaag catcatttcg cacaacctgg cgcagcgtaa agccgcagcg
     8041 gttgaggcgg aaactattgt cgctcaggaa accagcgaat ttatggcgtg gctgcgagca
     8101 caaagcgcca gcgaaaccat tcgcgagtat cgcagccagg cagagcacgt tcgcgatgag
     8161 ttaaccgcca aagcgttagc ggcccttgag cagggcggcg acgcgcaagc cattatgcag
     8221 gatctggcat ggaaactgac taaccgcttg atccatgcgc caacgaaatc acttcaacag
     8281 gccgcccgtg acggggataa cgaacgcctg aatattctgc gcgacagcct cgggctggag
     8341 tagcagtaca tcattttctt tttttacagg gtgcatttac gcctatgaag ccttctatcg
     8401 ttgccaaact ggaagccctg catgaacgcc atgaagaagt tcaggcgttg ctgggtgacg
     8461 cgcaaactat cgccgaccag gaacgttttc gcgcattatc acgcgaatat gcgcagttaa
     8521 gtgatgtttc gcgctgtttt accgactggc aacaggttca ggaagatatc gaaaccgcac
     8581 agatgatgct cgatgatcct gaaatgcgtg agatggcgca ggatgaactg cgcaaagcta
     8641 aagaaaaaag cgagcaactg gaacagcaat tacaggttct gttactgcca aaagatcctg
     8701 atgacgaacg taacgccttc ctcgaagtcc gagccggaac cggcggcgac gaagcggcgc
     8761 tgttcgcagg cgatctgttc cgtatgtaca gccgttacgc cgaagcccgc cgctggcggg
     8821 tagaaatcat gagcgccagc gagggtgaac atggtggtta taaagagatc atcgccaaaa
     8881 ttagcggtga tggtgtgtat ggtcgtctga aatttgaatc tggcggtcat cgcgtgcagc
     8941 gtgttcctgc tacggaatcg cagggtcgta ttcatacttc tgcttgtacc gttgcggtaa
     9001 tgccagaact gcctgacgca gaactgccgg acatcaaccc agcagattta cgcattgata
     9061 ctttccgctc gtcaggggcg ggtggtcagc acgttaacac caccgattcg gcaattcgta
     9121 ttactcactt gccgaccggg attgttgttg aatgtcagga tgaacgttca caacataaaa
     9181 acaaagcgaa ggcgctttca gtgctcggtg ctcgcatcca cgctgctgaa atggcaaaac
     9241 gccaacaggc cgaagcgtct acccgtcgta acctgctggg gagtggcgat cgcagcgacc
     9301 gtaaccgtac ttacaatttc ccgcaggggc gcgttaccga tcaccgcatc aacctgacgc
     9361 tctaccgcct ggatgaagtg atggaaggta agctggatat gctgattgaa ccgattatcc
     9421 aggaacatca ggccgaccaa ctggcggcgt tgtccgagca ggaataatgg aatatcaaca
     9481 ctggttacgt gaagcaataa gccaacttca ggcgagcgaa agcccgcggc gtgatgctga
     9541 aatcctgctg gagcatgtta ccggcagagg gcgtactttt attctcgcct ttggtgaaac
     9601 gcagctgact gacgaacaat gtcagcaact tgatgcgcta ctgacacgtc gtcgcgatgg
     9661 tgaacccatt gctcatttaa ccggggggcg agaattctgg tcgttgccgt tatttgtttc
     9721 gccagcgacc ttaattccgc gcccggatac ggagtgtctg gtggagcagg cactggcgcg
     9781 gttgcctgaa caaccttgcc gtattcttga tctcgggacg ggtaccgggg cgattgcgct
     9841 tgcgctggct agcgagcgcc cggactgcga aattatcgct gtagatcgta tgcctgatgc
     9901 tgtctccctg gcacaacgta atgcccagca tctggcgatc aaaaatatcc acattctgca
     9961 aagcgactgg tttagcgcgc tagccgggca gcagtttgcg atgattgtca gcaatccgcc
    10021 gtatattgac gagcaggacc cacatcttca acaaggcgat gtccgctttg agccgctcac
    10081 tgcgctggtt gcggcagaca gtggaatggc agacatcgtg catatcatcg aacagtcgcg
    10141 taacgcgctg gtatccggcg gctttctgct tctggaacat ggctggcagc agggcgaagc
    10201 ggtgcgacaa gcatttatcc tcgcggggta tcatgacgtc gaaacctgcc gtgactatgg
    10261 tgataacgag cgcgtaacgc tcggccgcta ttatcaatga caagtttttc tacactgctt
    10321 agtgttcatc ttattagtat cgcgctttct gttgggctat taaccttacg tttctggcta
    10381 cgttatcaga agcatccaca ggcatttgct cgctggacgc gcattgtgcc gccggttgtc
    10441 gatacggtgt tattgttaag cggcattgcg ttgatggcta aagcgcacat cctgccattt
    10501 tccgggcagg cacagtggct gactgaaaag ctgtttggag ttatcattta tatcgttttg
    10561 ggttttattg cactcgatta tcgtcgtatg cacagtcagc aggcgcgcat tattgccttc
    10621 ccgctggcgt tggtggtgct gtacatcatc attaaactcg ccaccacaaa agtaccgtta
    10681 ctggggtaag tcatgagatc gttagctgat ttcgaattta ataaagcgcc attgtgcgaa
    10741 ggcatgatcc tggcttgcga agcaatccgc cgcgattttc cctcgcaaga tgtttacgac
    10801 gaactggagc gtctcgttag tctggcgaag gaagaaatca gccagcttct gcctttagaa
    10861 gagcagctgg aaaaactgat cgcgctgttt tacggcgact ggggatttaa agcctcacgc
    10921 ggcgtttatc gtctttccga tgcattatgg ctggacccgg tgttaaagaa tcgacagggc
    10981 agtgcggtat cattaggtgc ggttttatta tgggtcgcga atcgtctcga tttgccgctg
    11041 ctgccggtga ttttccctac gcagctgata ttgcgcattg aatgtccgga tggcgaaatt
    11101 tggctgatta atccttttaa cggtgaatcg ttaagcgaac atatgctgga cgtatggtta
    11161 aagggaaata tcagcccgtc ggcggaactg ttttatgaag accttgatga agctgataac
    11221 attgaggtaa tccgcaaatt gctggataca ctcaaagcct cgttgatgga agaaaatcag
    11281 atggagctgg cgttacgcac cagcgaagct ttattacaat tcaaccctga agatccctat
    11341 gaaattcgcg atcgcgggtt gatttatgcg caactggatt gcgaacacgt tgcgttgaac
    11401 gatttaagtt atttcgttga acagtgtccg gaagacccga tcagcgaaat gatccgtgcg
    11461 caaataaata acatcgcgca taaacatatt gtgctgcatt aattaatcga cattttactc
    11521 aagattaagg cgatcctatg aaacaaaaag tggttagcat tggcgacatc aacgtagcaa
    11581 atgacctgcc gttcgtactg tttggcggta tgaacgtgct ggaatctcgc gatctggcga
    11641 tgcgcatttg cgagcactac gtaactgtga cccagaaact gggtatccct tacgtgttca
    11701 aagcctcttt tgacaaagcc aaccgctcct ccatccactc ttatcgtgga ccgggcctgg
    11761 aagaagggat gaaaatcttc caagagttga agcagacttt tggcgtgaaa attatcaccg
    11821 acgttcacga accaagtcag gcacagcccg ttgctgatgt cgtggatgtg attcagttgc
    11881 cggcgtttct tgctcgccag actgacctgg ttgaagccat ggcgaaaacc ggtgcggtaa
    11941 ttaacgtcaa gaaaccacag tttgtcagcc cgggacagat gggtaatatc gttgataaat
    12001 tcaaagaagg cggcaacgaa aaagtgattc tttgcgatcg tggtgctaac ttcggctatg
    12061 acaacctggt tgtcgatatg ctgggcttca gcattatgaa gaaagtgtct ggtaactcgc
    12121 cggtgatttt cgacgtgacc cacgcactgc aatgccgcga tccgtttggc gcagcttccg
    12181 gtggtcgtcg tgctcaggtg gctgagctgg cacgagccgg tatggcggta ggtctggcgg
    12241 ggctgtttat tgaagcgcat ccggatccgg aacatgcgaa atgtgatggt ccatccgcgc
    12301 tgccgctggc taaactggaa ccgttcctca agcagatgaa agcgattgat gatctggtga
    12361 aaggtttcga agaactggat accagcaagt aatctttttt gcttgaaaaa taaagtctta
    12421 gcgttctgcg ttaagacttt tttcatgggt gccagataca aaaa
//
LOCUS       DAJVUJ010000127        12052 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.127, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000127 DAJVUJ010000000
VERSION     DAJVUJ010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12052
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.127"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..182
                     /locus_tag="OQQ48_003129"
     CDS             <1..182
                     /locus_tag="OQQ48_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCS1953814.1"
                     /translation="FISALFFFNAVCIVSDNLLIIESFGEMAYNISYLTRVPGTNTLL
                     ACCCLLRPEEVNSEY"
     gene            172..345
                     /locus_tag="OQQ48_003130"
     CDS             172..345
                     /locus_tag="OQQ48_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HCS1953815.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(394..1466)
                     /locus_tag="OQQ48_003131"
                     /pseudo
     CDS             complement(394..1466)
                     /locus_tag="OQQ48_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
     gene            complement(1538..4282)
                     /gene="torS"
                     /locus_tag="OQQ48_003132"
     CDS             complement(1538..4282)
                     /gene="torS"
                     /locus_tag="OQQ48_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HCS1953816.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGRRLQLRQQQQQLSQQIVAAADEIARLAQGQAN
                     NATTSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPMLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALY
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSTVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDVPTALMGDPRRIRQVI
                     TNLLSNALRFTDEGQIVLRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAVGNAAQALETLQNSEPFAAALVDFDLPDVD
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQSLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCSSLGMRIASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLHKKDLNAI
                     "
     gene            4365..5354
                     /gene="torT"
                     /locus_tag="OQQ48_003133"
     CDS             4365..5354
                     /gene="torT"
                     /locus_tag="OQQ48_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HCS1953817.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHYTVQASTPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAGGYSQLATQQAQIDQCKQWGAEAILLGSSTTSF
                     PDLQKQVANLPVIELVNALDDPLVKSRVGVPWFQMGYQPGRYLVQWAHGKPLNVLLMP
                     GPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQEMLERHPEIDVVAG
                     TAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASDQMIWQGELAVEQA
                     IRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFYQHTSAAKK"
     gene            complement(5327..6019)
                     /gene="torR"
                     /locus_tag="OQQ48_003134"
     CDS             complement(5327..6019)
                     /gene="torR"
                     /locus_tag="OQQ48_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HCS1953818.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGECYFLAADVC"
     gene            6139..7311
                     /gene="torC"
                     /locus_tag="OQQ48_003135"
     CDS             6139..7311
                     /gene="torC"
                     /locus_tag="OQQ48_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HCS1953819.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEISHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDIAGKAHGDKKEEK"
     gene            7311..9891
                     /gene="torA"
                     /locus_tag="OQQ48_003136"
                     /pseudo
     CDS             7311..9891
                     /gene="torA"
                     /locus_tag="OQQ48_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
     gene            9888..10487
                     /gene="torD"
                     /locus_tag="OQQ48_003137"
     CDS             9888..10487
                     /gene="torD"
                     /locus_tag="OQQ48_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HCS1953820.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLAAAVNELENCIATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTA
                     LWQWLPEFAARCHQYDSFGFYAALSQLLLVLVECDHQNK"
     gene            complement(10580..10885)
                     /gene="cbpM"
                     /locus_tag="OQQ48_003138"
     CDS             complement(10580..10885)
                     /gene="cbpM"
                     /locus_tag="OQQ48_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HCS1953821.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(10885..11805)
                     /gene="cbpA"
                     /locus_tag="OQQ48_003139"
     CDS             complement(10885..11805)
                     /gene="cbpA"
                     /locus_tag="OQQ48_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HCS1953822.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
BASE COUNT         2894 a         3093 c         3190 g         2875 t
ORIGIN      
        1 tttttatatc tgccttattt ttcttcaacg ctgtatgtat agtaagcgat aacctgttga
       61 ttattgaatc tttcggggag atggcttata acatttctta cctgaccagg gtaccgggaa
      121 ccaacacctt actggcgtgt tgctgtcttt taagaccaga agaggttaac agtgaatatt
      181 gaagagttaa aaaaacaagc cgaaacggaa atcgccgact ttatcgcgca aaaaatcgcc
      241 gagctgaaca agaatacagg gaaagaagtc tctgaaattc gcttcaccgc acgagaaaaa
      301 atgaccgggc ttgaaagtta tgatgtcaaa atcaaaataa tgtgattttg tgaacatcac
      361 cccgtgcgag gtgatgttcc gcttgttgct aatttagtga tcaatcattg gcgcttgtgg
      421 aattaagcgt cggtacaatt cctccggcac cgggctttgc catactcccg catacattgc
      481 gtaaccaatc accgcgaaca taatccccag aaccagtagc gtcattaacc agccagacaa
      541 cgcaaaagct tttttatttg ccgcaggttt ttgcagtgaa aaggtcaatg ttgaggctac
      601 cggacatgac tctacgcaag tcatacagcc ggtacattcc actgttcgta cctgaattaa
      661 tttatctacc gggatccgtg atgggcaatt ttttgcgcat ttgccacagc cgatacaact
      721 ttcggcattg cgacgaatct taaacggcga taatagcgaa accacgccca tcagcgcgcc
      781 atatgggcaa agataacgac accaggcatg gcgaataaac aggctggcaa tcagcaaaac
      841 ggtcacgctg attaatgtcg cggtccccat atgacgaaag aaatcgagca tttttacgtc
      901 catcaccacg ctgtagggcg acaacataaa atagtgaatc gcctgagcgg gcatcaataa
      961 cgcgatatag agaaaaaaac tcaacagcaa atacttcacg ccgcgcagag gaatatccag
     1021 ccagcgggga aggacacatt gccgaccaaa cagtttgtta ccgagatcgc cgattaattc
     1081 agaaagcgta ccaaccgggc ataaccatga gcaaaaggcc tttttgagta atagactgat
     1141 gacgataaaa gcgaccaata acagcatcgc ggagcgtgga cggacggtaa ctgacctgtt
     1201 gtaaggctat atttcagatt catcagaccg gcaatcggta gccagccttc gatacctccc
     1261 ggtctggcga caaatgtcgt gctacttgcc gtttcgtaat agcgcaccca ataccagaac
     1321 gtgatggcaa tataaatatt cattgccaac agtaataatt gcgtcgcttt acgccaggtc
     1381 gtggcattgc gccagtcatt ccacggtaat ttgccgcccg tcgtgcctgg ccgccgctgc
     1441 cagcgggttc tttttttctc tgccatgatt ttgtcagtcc gttaagttgc ataccaaatg
     1501 ccactatttt agttgttctt aactggctga tattgattca aatcgcgttc aggtctttct
     1561 tatgcaacca tgcttccaga gcggcaacac tgcgtgtaat ttcttcgtgt ggaagggggg
     1621 cagataatgg ctgctgctcc agttgtgcgc atagctggct ggcgatacgc attcccaaac
     1681 tcgagcaact gctttttagc tgatgcgcgg cacgctttat tttctcgcta tcctgactgg
     1741 cgcgggcaat gtcgatttca tcgagaagcg gcagggcatg ttgtgtaaat aataccagcc
     1801 attcgtggat cttctccgtc cccattaact gagcatcttc attgagttgc gatacatcca
     1861 gcgattgatc gttattgact tgcagttgga gatagtgcgc cagtaactga ccgagcactt
     1921 cacgcggcac cggtttaggg ataatcccgc ggaataatga actggtacgc tggcgcagcg
     1981 tttcgtcaat gacatgggcg ctaaagccaa tcaaaaccag cgacggatat tgctgtgcca
     2041 gttgtcgggc aagcgtaatg ccgtcgacat ccggcagatc aaaatccacc agtgcggcag
     2101 caaacggttc gctattttgc agtgtctcta aagcctgcgc ggcattgcca acagcaacaa
     2161 cctgcgcacc actggtgttc agcatctcaa cggtaattcg ctgggttagc gggttatctt
     2221 caattaacag caaacgtaaa ccgtcaagac gcaccgcctg attgactgtt tttggcacgg
     2281 gtgccgtggc aacacgtaac ggcaagcgta aacaaaaaca gcttccaacc tccggcgtgc
     2341 tggtggcgct cagttcgccg cccatcgcct gggccagacg gctactgata gtcagtccca
     2401 gcccggtgcc gccgcgtttg ccgcttacct gcacaaatgg ctggaagatt tctgccagtt
     2461 tcgcgggatc aataccgcag ccgctgtctt ccacttcgac cagccattgc tcgccatcag
     2521 tgcggctacg taaaacgatc tgcccttcgt cagtaaaacg cagggcgttg ctcaacaggt
     2581 tggttataac ctgacgaata cgtcgcggat cgcccattaa cgcggtcggt acatcgtcgg
     2641 caattgctgt tgccaggcga atcgggcgac ctttcacccg tccgctcatt aattgcaggg
     2701 tactttccag cagcgggcgc ggttcaaagg gctcatcgct gaccgaaaca ttcttgccac
     2761 ctgcttcgat agccgaataa tcgagaatat cgttgaggat ggtcagcaac gattcgccag
     2821 agtcggtaat cgcccgcaaa tcatcacgct gggcgttaag tgcggggtta tctgccagca
     2881 gttgagcagt gccgagaata ccgtacagcg gtgtgcggat ctcatggctc atcgccgcca
     2941 gaaaggccga ttttgcctgg ctggcttttt ctgcttccgc ccgtgcctgt cggtgttcta
     3001 tcaccagttc ctgcaattca gctgtacgcg ctttgacctg cgccgccagc tgttcacggt
     3061 ggcgattcag tgcatgaaca gtgctgcgaa acgcatccat cagccgcccg atggtatcca
     3121 gctcccgtac gccagcggtt tccgggaaag gggagtcaat atcaccgtcc agcagccgtt
     3181 gcagcgcctg cgtttgttcg gcaagtggac gcgtgactga gcgataaacc acgcgccaaa
     3241 ggatcagaat cagtgcgcaa agtgaaacca tccccagcaa taacaggctg tattgcccgc
     3301 gtgcactggc tttttccaga tgcgccagtc cgtgctgatt acgcagctca atggtgtcga
     3361 ccagctgact gacttcgcta ctaaactgcg cgaactgggc gatgttattt tgtgcgagag
     3421 tttgtaggtg attgctgatt tcactgtcct gctgatacag cgccagcaaa tcgctatatt
     3481 ggctaacggt agttaacgtt gttgcgacct gcgcacgaac acccggatct tcaatgcgta
     3541 tttgccgacg ttgcagaatt ttcaccgcat tattgagctg cttttccagc attggtgcat
     3601 ttttctggat ctgctccagc cccagattca tcaccatttg ctgcacccgc agagcgctaa
     3661 ggcgcagttc attcatctgg ttaacatact caagatcgat atcaatcagc cgatcgagtg
     3721 cactttcagc agcctgacgc tgatcttgtt cgatcaaatc gtaaatcccg gcctgggtcg
     3781 ctccagcgga agttgtcgca ttattcgcct gaccttgcgc caggcgtgcg atctcatcgg
     3841 cggcagcgac tatctgctga ctgagttgct gttgttgctg gcgtagttgc agacgccgcc
     3901 ccaccagttc cccttgctga cgtaacgaac gggagatctc ctgctcctgt tgttcaatag
     3961 cggtggtatc aaaaccttgt tcccgtaacg cttgcagcaa cgcattaatc ttcaggcttt
     4021 gtgcggtgag cattcgcccc tgcgcctgcc acatcttttc gttatcggca ctggtcaggt
     4081 tctgcgcggc gaaaagttcc caggcgctgg cttcgctcaa ctggcgcgcc atattcatgg
     4141 taggaatcaa tgcctgagtg ttgtcttttt ccacctggct gataaagcgc aggttgtacc
     4201 atcccaccag ggtactggtc agggttaaca gcgccatcag ggcaaagccc atccagagtc
     4261 ttcgggtcag ggttaaattc acggtcggtg cactttaggt gaaaaaggtt gagtcgcaaa
     4321 gcagaatgca tctagcataa agccttatta ttgattaggc tatcatgcgc gtactgctat
     4381 ttttacttct ttcccttttc atgttatcgg cattttcggc tgataacctg ttgcgctggc
     4441 atgatgcgca gcattacacg gtgcaagcct ctacgccgct taaagccaaa cgcgcatgga
     4501 aactgtgcgc gctttatccc agcctgaaag attcatactg gttatcgttg aactatggta
     4561 tgcaggaggc aggcggctac agccagttgg ctacccagca agcacaaatc gaccagtgta
     4621 aacagtgggg cgcagaggcc attttgctcg gtagtagcac tacgtcattt cccgacctgc
     4681 aaaagcaggt agcaaatctg ccggtgatcg aactggtaaa tgctcttgat gatcccctgg
     4741 tgaaaagccg cgttggtgta ccctggtttc agatgggcta tcaacctggg cgatatttgg
     4801 tgcaatgggc gcacggtaaa ccactgaacg tgctgttgat gcccggaccc gataatgccg
     4861 ggggcagtaa agagatggtc gagggttttc gcgcagccat tgccggaagc ccggtgcgta
     4921 ttgttgatat tgcgcttggt gataacgata ttgaaatcca gcgtaacctg ttgcaggaga
     4981 tgctggaacg ccacccagaa atcgacgtcg ttgccggaac ggccattgcg gcagaggcgg
     5041 caatgggtga agggcgtaac ctgaaaacgc cgcttaccgt ggtgtcgttt tatctttcac
     5101 atcaggtgta tcgcgggctg aagcggggaa gagtgattat ggctgccagc gatcaaatga
     5161 tctggcaggg ggaactggcg gttgagcagg ccatcaggca attacagggg caatcggtgt
     5221 ctgataatgt cagcccaccg attttagttc tgacgccgaa aaatgccgac cgtgaacata
     5281 ttcgccgctc gctgtcacca gggggatttc gtccggtcta tttttatcag cacacatcag
     5341 cggctaagaa ataacattca ccatgttgcg tcaccagtaa atccgcgctg agtttatgac
     5401 gtaaacgacg aattaacaca tcgacggtgc gcaggtcagg gttttccacc cgacgcgcag
     5461 aaagcatacg tagcagacgt tcacggctga gaatttcgcc cggattcgtc acaaatgcca
     5521 ccaacatttc atactctgcg cgggtcagtt taatcggctc gccatcccgc tccagcgtat
     5581 ggcgcgacac attcaggcaa taaccggcaa agcgatagca gttgtcctga gtgtgcggtt
     5641 gagcttgtcg cgcgaggtcg attcgccaga gcagattttt cacccgtact accagttcgc
     5701 gcagttccag cggtttggtg acgtaatcgt ctgcgcccat ttccagccca acaatacggt
     5761 caatccgatc gctgcgtccg gtaaccagaa taatccccac cgttgagcgt tctcgcaggg
     5821 cgcgggttaa catcaggcca ttttcatcgg gtaagttgat atccagcaga attaaatcta
     5881 ccggctgatt ctgcataatt tcccgcagcc cggcaccgct cgctgtaacg gaaacggtat
     5941 acccctcctg agtgaagtaa gattgtaatc gcgcctgggt aaccggctca tcttcaacaa
     6001 taacaatgtg atgtggcatc agagggtttt actcattctg ttcatattct gccgtaagcc
     6061 gttcatcctg accagtgccg ctgttcatat ttgctcatta agatcgcttc actaaaccat
     6121 aattttacag gggctattat gcggaaactc tggaacgcgc tacgccgacc cagtgctcgt
     6181 tggtcggtac tggcgctggt cgcaattggg attgtgattg gcattgcgct gattgtattg
     6241 ccacacgttg ggatcaaagt caccagcaca accgaatttt gtgtcagttg ccacagtatg
     6301 caaccggtgt atgaagaata taaacagtcg gtgcatttcc agaacgcctc cggcgtgcga
     6361 gctgaatgcc atgactgtca tatcccgccg gatattccag gcatggtgaa gcgcaaactg
     6421 gaagcgagca atgatatcta ccagaccttt attgctcact ccattgatac acctgaaaaa
     6481 ttcgaagcca aacgcgcgga acttgccgag cgtgaatggg cgcgaatgaa agaaaacaac
     6541 tcggcaacct gccgctcctg ccataactac gatgcgatgg atcatgcgaa gcagcatcct
     6601 gaagcggcac gtcagatgaa ggtggcagcg aaagataatc aatcctgcat cgactgtcat
     6661 aaaggtattg cccaccagtt accggatatg agtagcggct tccgtaagca gttcgatgag
     6721 ctgcgcgcca gtgctaatga cagtggtgac acgctgtact ctattgatat taagccaatt
     6781 tatgcggcga aaggcgataa agaagcctct ggttctctgc tgcctgcttc ggaagtgaaa
     6841 gtccttaaac gtgacggcga ctggctgcaa attgaaatta ccggctggac ggaaagcgcc
     6901 ggacgtcagc gtgtactcac ccaattccca ggtaaacgca tctttgttgc ctcgattcgt
     6961 ggtgatgtgc agcagcaggt aaaaacgctg gagaaaacca ccgttgccga caccaatacc
     7021 gagtggagca agttgcaggc cactgcgtgg atgaagaaag gtgatatggt gaacgatatc
     7081 aaaccgatct gggcttatgc ggattcgttg tacaacggca cctgtaacca gtgccacggc
     7141 gcaccggaaa tctctcactt tgatgctaac ggctggattg gcacgctcaa cggcatgatt
     7201 ggctttacca gtctcgataa acgtgaagaa cgcaccttgt tgaaatatct gcaaatgaat
     7261 gcgtctgaca tcgcaggtaa ggctcacggc gataagaagg aagaaaaata atgaacaata
     7321 acgatctctt tcaggcatca cgtcggcgtt ttctggcaca actcggcggc ttaacagtcg
     7381 ccgggatgct ggggccgtca ttgttaacgc cgcgcagtgc gactgcggcg caagcggcgg
     7441 atgtcatctc gaaagagggc attcttaccg ggtcgcactg gggggctatc cgcgcgacgg
     7501 tgaaggatgg tcgctttgtg gcggcaaaac cgttcgaact ggataaatat ccgtcgaaaa
     7561 tgattgccgg attgccggat tgccggattg ccggattgcc ggattgccgg attgccggat
     7621 cacgtacaca acgcggcgcg tattcgttat ccgatggtac gcgtggactg gctgcgtaag
     7681 cgccatctca gcgatacctc ccagcgcggt gataaccgtt ttgtgcgcgt gagctgggat
     7741 gaagccctcg acatgttcta tgaagaactg gaacgcgtgc agaaaactca cgggccgagt
     7801 gccttgctga ccgccagtgg ttggcaatcg acggggatgt tccataacgc ttcggggatg
     7861 ctggcgaaag ctattgcctt gcatggtaat agcgttggta cgggcggaga ttactctacc
     7921 ggtgctgcgc aggtgatcct gccgcgcgta gtcggttcga tggaagtgta tgaacagcaa
     7981 acctcctggc cgctggtatt gcagaacagc aaaaccatta tgctgtgggg ctccgatttg
     8041 ctgaaaaacc agcaagcgaa ctggtggtgc ccggatcacg atgtttatga atattacgag
     8101 caactaaaag cgaaagtcgc cgccggtgaa attgaggtca tcagcatcga tccggttgtc
     8161 acatccaccc atgagtatct ggggcgcgag catgtgaagc acattgcggt taacccgcaa
     8221 actgacgtgc cgctgcaact ggcgctggcg tatacgctgt acagtgaaaa cctgtacgac
     8281 aaaaacttcc tcgctaacta ctgtgtaggt tttgagcagt tcctgccgta tctgctgggt
     8341 gagaaagacg gtcagccgaa agatgccgca tgggctgaaa aactgaccgg cattgatgcc
     8401 gaaaccattc gtgggctggc gcggcagatg gcggcgaaca gaacgcaaat tattgctggc
     8461 tggtgcgtgc agcgtatgca gcacggtgaa cagtgggcgt ggatgattgt ggttctggcg
     8521 gcgatgctgg ggcaaattgg cctgccaggt ggtggttttg gttttggctg gcactacaac
     8581 ggcgcaggca cgccggggcg taaaggcgtt attctgagtg gtttctccgg ctctacgtcg
     8641 attccgcctg ttcacgacaa cagtgactac aaaggctaca gcagcaccat tccgattgcc
     8701 cgttttatcg atgcgatcct cgaaccgggg aaagtgatca actggaacgg taaatcggta
     8761 aaactgccgc cactgaaaat gtgtattttt gccggaacta acccattcca tcgccatcag
     8821 cagatcaacc gcattattta aggctggcgc aagctggaaa cggttatcgc catagataac
     8881 cagtggacct caacctgccg ctttgccgat atcgtactgc ctgcgaccac gcagtttgag
     8941 cgtaacgatc tcgaccagta cggcaaccac tccaaccgtg gcattatcgc catgaaacag
     9001 gtggtgccgc cgcagttcga ggcgcgcaac gactttgata ttttccgcga gctgtgccgt
     9061 cgctttaatc gcgaagaagc ctttaccgaa gggctggacg aaatgggctg gctgaaacgc
     9121 atctggcagg aaggtgtaca acaaggcaaa ggacgcggcg ttcatctgcc agcgtttgat
     9181 gacttctgga ataacaaaga gtacgtcgag tttgaccatc cgcagatgtt tgttcgccac
     9241 caggcattcc gcgaagatcc ggatctcgaa ccgctgggca cgccgagtgg cctgattgag
     9301 atctactcga aaaccatcgc cgatatgaac tacgacgatt gtcaggggca tccgatgtgg
     9361 tttgagaaaa tcgaacgctc ccacggtggg cccggctcgc agaagtatcc gttgcatctg
     9421 caatctgtgc atccggattt ccgacttcac tcgcagttat gtgagtcgga aactctgcgt
     9481 cagcaatata cggtagcggg taaagagcca gtattcatta acccgcagga tgccagcgcg
     9541 cgcggtattc gtaacggtga tgtggtacgc gtctttaacg ctcgcggtca ggtgttggca
     9601 ggggcagtag tttctgaccg ctatgcaccc ggcgtggcac gaattcacga aggggcatgg
     9661 tacgatccag ataaaggcgg cgagcctggt gcgctgtgca aatacggtaa ccccaacgtg
     9721 ctgaccatcg acatcggtac ttcgcagctg gcgcaggcga ccagtgcgca cactacgctg
     9781 gtggaaattg agaagtacaa tggagcagtg gagcaggtga cggcgtttaa cggccctgtg
     9841 gagatagtgg cgcagtgcga atatgttccc gcgtcgcagg tgaaatcatg accacgctga
     9901 cagcacaaca gattgcctgt gtttacgcct ggctggcgca gttgttctcc cgtgaactgg
     9961 acgatgaaca actgacgcaa atcgccagtg cgcagatggc ggaatggttt tcgttgctga
    10021 aaagcgaacc gccgctggct gcggcggtga atgagctgga aaactgtatt gccacgctga
    10081 cagtacgtga cgatgcccgt ctggaactgg ccgctgactt ttgcggcctg tttttgatga
    10141 ctgataagca ggcggcgctg ccgtatgcat cggcctacaa acaggacgag caagagatta
    10201 aacgcttgtt agttgaggca gggatggaaa ccagcggcaa tttcaacgaa ccggcagacc
    10261 atctggcgat ctatctcgag ttgttgagtc atctgcattt ttcgctggga gaggggaccg
    10321 ttcctgcgcg aagaatcgac agtttgcggc aaaaaacact gacggcgctg tggcaatggt
    10381 taccagagtt tgctgcgcgt tgccatcagt atgacagctt tggtttttac gcggcactaa
    10441 gccagttatt gctggtgtta gtggagtgcg accaccaaaa caaataacgt cgtttatgcg
    10501 cctgaaaaga cgcgtttagc gtcgcatcag gcattatggc gcagttgccg gatgcgtcat
    10561 cacatccggc aaacacacat cacggatgag ctacaaaccg ggaaagccgc tggcgtagca
    10621 ggcggttttc ctgcttcagg tgtgcaatat catccattaa cgtcagcgcc accgcgatcc
    10681 ccggccagtc cagagccagt tcatgacgca ggcgtaccgc gcgttgcacc acaatggcgg
    10741 catggtcgtc aaatacccag gttgtttcct ggatctcacg cggttcaacc acccccaaac
    10801 cgacaatttc attcaactcc tcttcagaga tgccggtatg caggcaaaat tcggtaatag
    10861 taaaagtcac cgtaacatta gccatcatgc tttcccccaa tctttacgtg gatcaaaaga
    10921 cgactgggcg tctgccagtt gctgccacag cgcggcagtg ttttcatccg gtttcggcgg
    10981 catcacgatt ttcagtaccg catacagatc gccggtctgt tttttgctca ccagaccttt
    11041 gcctttaacg cgcaatcgtt gcccggcctg gctgcctggc gggatagtca gcaaaatgct
    11101 ttctttcagt gttggaacgg tgactttagc acccagcgcc gcttcccacg ggctaaccgg
    11161 caccacaatt tccagatcct ggccgacaat atcgaacagc ggatgtggcg caatatgaat
    11221 caccagccac aaatcgccat ttggaccacc gttttcgccc ggcgtcccct ggcctttcag
    11281 acggatgcgt tgaccattgc cgacgcccgc cgggatcttc acattcagcg ttttcgggat
    11341 ttcctgttcg atcatgccaa aggcgttata aaccggcagg ttatagctga tggtacgctt
    11401 atgctcagtc agcgtttctt cgaggaatac cgccacttcg atttcaatat cgtggccgcg
    11461 tgcggcgggg cgttgacggc tctggcgggc atgctgaccg aaaattgacg agaagatatc
    11521 gtcaaaatct tcggcattaa aactctgacc gtcgccatga tggaactgac ggttaaattg
    11581 cggatcgttg cgatgttgcc acatctgatc atactcagcg cgacgttgtt catcacttaa
    11641 cacttcccag gcttcagcga cctctttgaa gcgggcttcg gcatccggtt ctttgctgac
    11701 atcaggatgg tatttacggg ccagtcgacg ataggcggtc ttgattgtct tgagatcgtc
    11761 cgtcggtttc acgcccatga tggcgtaata atcctttaat tccatagcgt tatctcgcgt
    11821 aaatcaacac aaattgaagg aacccctgta aggtaactcc tataagtgta gggtaatcct
    11881 caaaatttca tatgccaaca cagaatatgt tattgaaatc atcgtggaga ggaggtcgcc
    11941 atcaatatgg gttgctgaac atattttaaa caggtgaaaa agggtgagcg atttttgata
    12001 gttgaaccag gcactttaag tttaactagg gcgtcattat ttattaaatt tt
//
LOCUS       DAJVUJ010000128        11984 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.128, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000128 DAJVUJ010000000
VERSION     DAJVUJ010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11984
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.128"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(4..324)
                     /gene="chbB"
                     /locus_tag="OQQ48_003140"
     CDS             complement(4..324)
                     /gene="chbB"
                     /locus_tag="OQQ48_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HCS1953823.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(622..960)
                     /gene="osmE"
                     /locus_tag="OQQ48_003141"
     CDS             complement(622..960)
                     /gene="osmE"
                     /locus_tag="OQQ48_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HCS1953824.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            1162..1989
                     /gene="nadE"
                     /locus_tag="OQQ48_003142"
     CDS             1162..1989
                     /gene="nadE"
                     /locus_tag="OQQ48_003142"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCS1953825.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALACPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPQQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            2219..3106
                     /gene="cho"
                     /locus_tag="OQQ48_003143"
     CDS             2219..3106
                     /gene="cho"
                     /locus_tag="OQQ48_003143"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HCS1953826.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQTIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEDATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(3066..3641)
                     /gene="ves"
                     /locus_tag="OQQ48_003144"
     CDS             complement(3066..3641)
                     /gene="ves"
                     /locus_tag="OQQ48_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HCS1953827.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFAADQVVKAKLTEGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(3844..4314)
                     /gene="spy"
                     /locus_tag="OQQ48_003145"
     CDS             complement(3844..4314)
                     /gene="spy"
                     /locus_tag="OQQ48_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HCS1953828.1"
                     /translation="MRKLTALFVASTLALGAANLAHAAAPADAKPMMHHKGKFGPHQD
                     MMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIVSDTFDKAKAEAQIAK
                     MEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATAE"
     gene            complement(4644..5612)
                     /gene="astE"
                     /locus_tag="OQQ48_003146"
     CDS             complement(4644..5612)
                     /gene="astE"
                     /locus_tag="OQQ48_003146"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HCS1953829.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGDETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(5605..6948)
                     /gene="astB"
                     /locus_tag="OQQ48_003147"
     CDS             complement(5605..6948)
                     /gene="astB"
                     /locus_tag="OQQ48_003147"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HCS1953830.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKTLADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(6945..8423)
                     /gene="astD"
                     /locus_tag="OQQ48_003148"
     CDS             complement(6945..8423)
                     /gene="astD"
                     /locus_tag="OQQ48_003148"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HCS1953831.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLESNKAELTAIIARETGKLRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGRANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLARLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQLETSLLTPGIIEMTGVAGVPDEEVFGPLLRVWRYDSFEEA
                     ILMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(8420..9454)
                     /gene="astA"
                     /locus_tag="OQQ48_003149"
     CDS             complement(8420..9454)
                     /gene="astA"
                     /locus_tag="OQQ48_003149"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HCS1953832.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWHNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAVEKTA"
     gene            complement(9451..10671)
                     /gene="astC"
                     /locus_tag="OQQ48_003150"
                     /pseudo
     CDS             complement(9451..10671)
                     /gene="astC"
                     /locus_tag="OQQ48_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
     gene            11117..11923
                     /gene="xthA"
                     /locus_tag="OQQ48_003151"
     CDS             11117..11923
                     /gene="xthA"
                     /locus_tag="OQQ48_003151"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HCS1953833.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
BASE COUNT         2939 a         3248 c         2965 g         2832 t
ORIGIN      
        1 aaattaattt gctgcggctt ttttaatcgc tgcaaccgca gccttaagca cgcctaaacc
       61 atcgactttg ccataaagca gcgagtcaat tacttcaacc ggtttgttgg gtaacaaacg
      121 ctggatttcg ggcaacatat aagcaatctg cggccctaat aacacgacat cggcattctg
      181 acctttttca ccagccagtg tttccggaaa tgcttcaata atgaccggaa cttcatattt
      241 ttctgcctgt gcgcgcattt ttgataccag taaagaggta gacatgcccg cagaacaaaa
      301 cagataaatg tgtttctttt ccataaaact gccctcatcg acgattatct gtcagccaga
      361 cactccgcaa gccttaacct gcttccatgc tctgggtaac ttgcgaaacc aacatgactt
      421 tttttgtggc tgaaaggagt atacggtata gacctgagca atagtatttc tttgacctta
      481 taaattgtct ctccttgacc tgcggttatg accctcttca catttcgggc aaataattcg
      541 cgaagataat taagaactca ggccaaaaaa accggcgcaa tggccggttt ccgttgttac
      601 tcaagctttc aaacgaagtg attacttcgc agcctgtgga tcagtgtcgt attcagcaca
      661 ggtctgataa ccggagttga tgacatgtcc ggtatcatct aacgcgacaa agtaggtttc
      721 tgctttacca tcacgttgac ccaggatgta ggtctggcaa gtaccacgag catggatcat
      781 gctcacttca gacgaaggtt tacccgcaat ttgtgcaacc tgcgcccggc tcatgccttt
      841 tttgacgtct ttcaccacag gctgtacaaa ctggtctttg gtacgatcat aagccgtaca
      901 acccgccagc atggttaata ccgccgctgc actcagaatt cctgccatat tcttgttcat
      961 attccgtcct cttgtttatc agcgtgttag ataagcctgg aatacattgg gcgctttttc
     1021 aagcccgtga acgaaacggc tccgctttca gaggattcct gtatgacgtt ttatccacca
     1081 ttcaacccgc tgtcgcttgt cgtttcagta gcgacgggtt agctttaagg aagttttgtc
     1141 ttttctgtct ggaggggttc aatgacattg caacaacaaa taataaaggc gctgggcgca
     1201 aaaccgcaga ttaatgctga agaggaaatt cgtcgtagtg tcgattttct gaaaagctac
     1261 ctgcaaactt atccgttcat taaatcactg gtgctcggga tcagcggcgg tcaggactcc
     1321 acgcttgccg gtaagctgtg ccagatggcg attaatgagc tgcgccagga gaccggcaac
     1381 gaatcactgc aatttattgc cgtacgcctg ccctatggtg ttcaggccga cgaacaagat
     1441 tgccaggatg ccattgcctt tattcaaccg gatcgcgtat taaccgttaa tatcaagggc
     1501 gcggtattgg ccagcgaaca ggcgctgcgc gaagcgggca ttgaactgag cgattttgtc
     1561 cgtggcaatg aaaaagcgcg tgagcggatg aaagcacaat atagcattgc gggtatgacc
     1621 agcggtgtcg tggtgggcac cgatcatgca gcagaagcta ttaccggatt cttcactaaa
     1681 tatggtgacg gcggtacgga cattaatccg ctgtatcgtc tcaacaaacg tcagggtaaa
     1741 cagttactgg cggcattagc ttgcccggaa cacctttata agaaagcgcc aacggccgat
     1801 ctggaagatg atcgcccttc tctgccagat gaagtggcac tcggcgtgac ctatgacaat
     1861 atcgacgact atctggaagg gaaaaacgta cctcaacagg tcgccagaac aatagagaac
     1921 tggtatctga aaaccgaaca taaacgccgt ccgccaatta ccgttttcga tgatttctgg
     1981 aaaaagtaat aatttgcaca tattggattg tgcgaaaaag agtaatttgt tcacgccgga
     2041 tgcggcgtga acgccttatt cgacctataa aactatgcaa attcaataca ttgcaggagt
     2101 cgaataggcc tgacaggcgt agcacgtcag acggtgtaac ctttgtcatc gacccgcctc
     2161 ttttttaatc gcttcccgcc tgttacactg gatagataac cagcattcgg agtcaacagt
     2221 ggtacggcgt ttaacttctc cgcggctcga atttgaagct gcggcaattt atgaatatcc
     2281 cgaacattta cgttcattcc ttaatgactt acccacccga ccaggggtgt atctgtttca
     2341 tggtgaaagt gacaccatgc cgctctatat cggcaaaagc gttaacatcc gcagccgcgt
     2401 cctttctcat ttacgtaccc cggatgaagc cgccatgcta cggcaatccc gacggatcag
     2461 ctggatatgt accgccggtg aaatcggcgc tctgctcctt gaagcgcgat taatcaaaga
     2521 acaacagccg ctgtttaata aacggttgcg ccgcaatcgt cagctctgtg ccctgcaatt
     2581 aaatgaaaag cgcgtcgatg tggtgtatgc caaagaggtg gatttttcac gagcccccaa
     2641 cctgtttggc ctgtttgcca ataggcgcgc agctttgcaa gcattgcaga ccatcgctga
     2701 tgaacaaaaa ctttgttatg gcctgctggg actggaaccg ttaagtcgcg gtcgtgcatg
     2761 ttttcgttca gcgctaaaac gttgcgccgg agcatgctgc ggtaaagaga gccatgagga
     2821 acatgcgcta cgcttgcgcc agtctctgga gcgtttgcgg gtggtgtgtt ggccttggca
     2881 aggggcggtg gcgctgaaag aacagcaccc ggaaatgact caatatcata ttattcaaaa
     2941 ctggctgtgg ctgggggcgg ttaattcgct ggaagacgcg acaacgttaa ttcggacacc
     3001 cgccgggttt gatcacgacg gttataaaat tctttgtaag ccgctgcttt ccggtaacta
     3061 tgaaattact gaacttgatc cggcgaatga ccagcgagcc agttgatttc actgaacaac
     3121 agtttacctt gcggttgtaa aagccgcagg gtgtgcctgc catcgaacca gcacgcgccc
     3181 tggtcggtag tgagcaattt atcgcccagt tgccacgccc cgttaatcac aaacaccacc
     3241 ccgccgcgtg agccaaaggt agtaaacgta cgttcggcaa ttctgacttt cgccttacag
     3301 acatccaggc gtgtcatgat gttgaaatcc atcgacatct gcccttccgt cagtttcgct
     3361 tttaccacct ggtccgctgc aaaggcaaaa ggctgtaacg gttttaaggt atggttaaag
     3421 cggtctgcgc tttcaaggag catctcgccg ccttccagca acgtcactat cctttccatg
     3481 ccgggaaaca aagaaaactc cccattcgcc gcgatggatg caatgctggc acgccagtaa
     3541 aaatcacgtt ttgccggtgg gaaagtgcaa atttcgcgcg tttcaccggc agcatttcgc
     3601 cacagattca ccgacatttt acgcatatca aagtattcca tactcgctcc cggcccgttc
     3661 ttttcattgt tatcgttcag gtaaccgtgg atggttatcg gcaactcggg tgatttacgt
     3721 tagtggtgat caggcttttt ttacaactgt ttgagaagag aaaagaaaac cgccgatcct
     3781 gtccaccgca ttactgcaag gtagtggaca agaccggcgg tcttaagttt tttggctgaa
     3841 agattattca gcagttgcag gcattttacc ttttgccgct ggacgttctg tcagacgctt
     3901 ctcaaaatta gcattaaatt gctttttctg ttccggcgtc aggatgttgt aaattttgtt
     3961 ctgggtttcc atgtgcgcca gcatgttagc tttgcgctgt tcttccattt ttgcgatctg
     4021 cgcttcagct tttgctttat cgaaggtatc gctgacaatg atgtcatgca ttgcgcggcg
     4081 ttcttccagc ggcggacgtt tcatctggtc acgctggcct ttcatgattt cgcggatctg
     4141 ctgtttctgc gcgtcggtca ggttcaggtc tttgaacatc atgtcctgat gcggaccgaa
     4201 cttgcctttg tggtgcatca tcggcttcgc gtcagccggt gctgcggcat gggccaggtt
     4261 agccgcgcca agagccaggg tagaggcaac aaacagtgca gttaatttac gcatattcta
     4321 tatccttcct ttcagttatt tattacggct ttcttaagta gcgtgccgtg ttgacaagat
     4381 taactttact gactttagcg tcaattaatc agagcaacgg taaaacaatg aaagtgtaaa
     4441 aaacactttt gcgccaatta tggagaaaaa aagaaaattt gatggagagt gatgagagaa
     4501 tattacaaca caatgatttt gcagagatta tgaagaacta tactggatga ctggtgataa
     4561 ataaagcaaa taaccaggat taatctgtat taatttataa gaaagcaact taatacccgc
     4621 agaatgattt ctgcgggtaa gtattagctt attttttcga gcattaatcc cgcgcgtaat
     4681 cccaacgcta ccaacggatt agggaataac acatactcta catcatgggt tacggtaaaa
     4741 cgttcctctc cgtcctgcgc cagcaatgtt cctttctcaa acggcataaa attcagcgtg
     4801 tcacttgcca tatgcatttc gaaggacggc gagtggcgag taatttgcga aaccacccga
     4861 taacggagcg gcggtgttct cacgataccg acactctcac cagatagcag cgcagcaatt
     4921 gcgctggcag ttactgcaaa ctggcgaaga tcgttttgcc caaagggcaa cgctttgcca
     4981 agttccagcg tacaggccag cgcgccaaaa tgtctggcgc tgaaatgggt aaacgtacca
     5041 ccaggttcct gatggaacac cagcgcctcc agccccgccg cacccagcca cgtcagaaat
     5101 ttctcgtccc agggaatgtc gcgttgcggt aatacaccga actgcggatg caaggagcca
     5161 cgaattgcgg tatgtagatc aaggtgccag cgcacagatt ctttgccctg gtcataaaaa
     5221 tcttccaggc actgttccag ttcgcgcgcc cgacaggttt ctccgctttc agcaaatagc
     5281 tgccaacgac cgccaaacat tcgattcata tcgctatggc aataacgttt cccttgcttc
     5341 agcgcaggag gattcccgag gatcaccagc aaccgccaac gtaacgggat ctcgccgtga
     5401 gatatcgcgc caagcaacgc gtccagcatt tccacaggtg ccgtctcatc accgtggatc
     5461 cccgcagaaa tcaccagcgc gccttgcggt ggcgttaatg gcgtcagttc cagcacacca
     5521 tcgcccagcc agcgccagcg aacgccgttg atttctcgct cggtgataac cggtttttta
     5581 cccgttaagg tcagagcaag aaaattatcc attgccccct ccctcgcgct ggaacggata
     5641 aaccgaaccg agattcagta attggctcaa tacatccagt gcttcccgcc cttcgcgcag
     5701 caattgcggg tcggccagat cggcagcagt aagacgatcg cggtagtaac gatccaccca
     5761 gtcattgagc gcattaaaca aggtatcgtt catcatcacc gccggattca ccgcccggcg
     5821 ttcttcttct gtcaatacca cccgcaaccg caggcacgcc gggccgccgc cattcgccat
     5881 gctttcacgt aagtcaaaga cttttagttc gctaatcggg ttgtcagcgg caaggagttc
     5941 attgagataa ccccatactc cggcgtgttc ccgacactcc tgaggcagca ccaacatcat
     6001 ggaaccatca tcgcggctca acagttggct gttaaacaga taggtagaca ccgcatccga
     6061 cacggaaacc tgagttgccg gaacttctat cgccataaaa ccgttcaccc gcgcccgcag
     6121 gtttgccagt aactgtgact ggcgagcgaa cgcctgttgg tggcaaaaca gcacctggcg
     6181 gttacttacg gcaatcacgt cattatgaaa aacaccctgg tcgataacgt ccgggttttg
     6241 ctgggcgaaa atcacctgtt ggggattcac ctgattaagc cttgccaccg cctcgctggc
     6301 ttcgcgagtc tgtcgcgccg gataacggga aggccgggta tcattgcctt cttctcgccc
     6361 gtagacaaaa agttgcatac ccggctcacc gtaatgaccg ccgagacgat tgtggtttgc
     6421 cgccccctca tcaccgagca acgctacctg cggcaacgcc gaatggacgc taaatttctc
     6481 ttcgtcgtta aaaatagctt ttaacagcga ttcagtgacg ggcgcttcca gcgaacggtg
     6541 aaatttattg ttcaggttgg caacggtgag atgcactttg ccatccagcg tatcggcaga
     6601 tggcgcgatc gttgccgcat tggctaccca cattggcgaa gcggagctga cgctggaaag
     6661 ccagtgcggt gcctggcgtg caactttttc cagtacctgc tcatcgctac cactgaatcc
     6721 caactgacgc agcaccggaa taaacggacg ctcgtgcggc gggatcacgg cctgggggaa
     6781 tcctgcatcg gcaagggttt tcattttcag taagccctgt ttcgccgcca gtcgcgggtt
     6841 agacacctga aaacggtgac gggtagaggc ttcattacca aacgacaggc cagcgtaatg
     6901 atgcgtcagc cctaccagcc cgtcgaaatt aacttcccag gcgttcatcg caccacctca
     6961 tcggaaaaat ccagcccggg gttaagcgtt gcgggcaaag ttaacgagtc cgactccagg
     7021 ctcgccatag gccatgcgca gtaatctgcg gcataccagg cgctggggcg atggttaccg
     7081 gatgcaccaa tgccgccgaa tggcgcggta ctggcagcac cagtaagcgg tttgttccag
     7141 ttaacaatcc ccgcccgcgc ctccagcaac agttgatcga atttttcccg ttcgggggaa
     7201 accagaccgc aagagagtcc gaagcgagtg ttattcgcca ttagaatcgc ttcctcgaaa
     7261 gaatcataac gccagacgcg caataacggt ccgaacacct cttcatctgg tacgccagca
     7321 acgcctgtca tttcaatgat ccccggcgtc agcaacgatg tctctaattg taataagcgc
     7381 ggcgcaagca gggttcgtcc gcccatcgct tccagttgct gccaggcagt aaccacctgc
     7441 tgtgcggcct gttcagaaat cagcccgcca ataaacggct gcggttcgtc atcccagttg
     7501 cccggcgtta atcgctggct gacggcaacc agacgagcaa gaaacgcatc gccctgcgct
     7561 ccgcttttga gcaataaacg gcgggcgcag gtgcagcgtt gcccggctgt gacaaacgcc
     7621 gactgaatgg tcagatggac agccgcgtcg atatccgcca cctcatcgat aattagcgga
     7681 ttattaccgc ccatctcaag ggcgagaatt ttctccggct gaccggagag ctggcgatgc
     7741 agctggtagc cggtattggc gcgaccggta aacagcaaac cgtcgagatc ctccagcgca
     7801 ctcagcgcct gacccgtttc acgcccgccc tgcaccaggt tcagcacgcc cggcggcaag
     7861 ccagcctgct gccataaacg cattaccgct tcgccactcc acggtgtcag ttcgctgggt
     7921 ttaaagataa tggtgttacc tgccagcaat gccggaacga tatgtccgtt cggcaaatga
     7981 ccagggaaat tatacggccc aaacaccgcc agcacgccgt gcgggcgatg tcgcaggctc
     8041 gccgcaccgt ccggcatttc actacgctgc tcgccggtac gaacgtgata cgccttaatt
     8101 gatatcgcga ttttattgat catcgccgtc acttcggttg ccgcttccca gcgcagctta
     8161 cccgtttctc tggcaataat cgcggttaat tcggctttat tgctttccag caatccggca
     8221 aagcgttcga caacgacctg acgttcagcc aatgagagcc gcgcccagcg cggaaacgcc
     8281 gcacgggctg cccgacaagc ctgttcgacc tgagcggcat cggcatcatt gccttgccat
     8341 aacacctcgc ccgataccgg attacgcttc acacgcgatg cgccctggcc cgttatccag
     8401 tcaccgttaa tccataaagt catgctgttt tctccactgc gcacaggcgc accagacgaa
     8461 cgcgatcccc tgcgtggcat ttgagggcat ccagttgtgc ggcggttaaa atcaaacgct
     8521 cggttgccgg atcggtacgc gccagcacca cgcggaaatg gtgataattt tcattggcga
     8581 ccaggcaggc cggaaaatcg ccctgcgcag gctgcccttc tgccacttcc accagccgac
     8641 ttttacggat ggcgcgcacg cggtcgatgt cacactcaag cgttggcccg ccgtcgaaga
     8701 tgtcgatata gttgcggtaa cgaaaacctt ctttctccag caccgcgcgg gcaggcgcgg
     8761 tttgcggatg tacctgaccg atgacgtcct gggcttcctg ggataaaaag tgggtataga
     8821 tcggatgttt cggcatcagt tctgcaataa atgccttttg cccggtgccg cagagaaaat
     8881 cggcgcggct aaaatccatc gaaaagaagc gtttaccgag gctttgccag aacggtgaat
     8941 agccgtgttc gtcaatcacc ccgcgcattt cagcaaccac tttgtcatta aacttgtcgc
     9001 gaaaagccgc cataaacata aagcgcgatt tcgacagcaa atagccgttg ccctctttgc
     9061 gccagtccgg gtcgagaaac agcgtgcaca gctcgctgct gccggtgtga tcgttactaa
     9121 gaaacagcgt cggcaatgcg ttatagacat tcagctcttt tgaggcgtga accaacgtgc
     9181 cgacccgata gttgtgccag ggatcgttca gcccaaccgc cacctcaatg gcgcaaatcc
     9241 ccgccacggt gcctgtctcg ctatcttcca gcacgaacac atagccctgc tcgcttttgg
     9301 gcagttcgcc ttgccaggtt ttgattgccc tttcgatacg cgcggaaagc gtggcttcat
     9361 tagcgggaag cgacgtcagg ccgccgcccg ttttgctggc aagctgcatc agcgccgaga
     9421 catctgagcg ctcaacggga cggatgacca tcatgatgaa cctcggctaa caaaatgttc
     9481 gcaagcagct gcaaagcgat ccagtccggt cgtcacctct tcttcgctga cattgagcgc
     9541 aggcgcaaaa cgcaccacgt tgccacccgc aatcagtacc ataacgcctg ctttcgccgc
     9601 ctcctgagag atctgtttcg cttgtccggc gtaatcggca ttcagtacac agccaatcaa
     9661 caaacctaag ccgcgaactt cactgaacaa accgtagcgg tgattaatgg tattaagacg
     9721 ctcaacaaac cagtcgtgac gctgtttaac gccattaagc atctctggtg tgttgatgag
     9781 ctccagcact ttgcccgcca ccgccgaggc cagcggattg ccgccatagg tggtgccatg
     9841 agtgccaacg gtcatcacgc tggcgcactc ttcggttgtt aacaacgcac cgaccgggaa
     9901 atcgccgccc agcgctttgg cggtagttaa cagatcaggt gtcacgccgt agtgcatata
     9961 ggcatacagt tccccggtgc gcccgacgcc ggtttgtact tcatcaaaaa tcaacagcgc
    10021 attgtggcgg tcacacaatt cacgcagacc ttgtaaaaac gcgttgctgg ctggcaccac
    10081 accgccttcc ccctggatgg gttagacaat caccgcacag gtggcgtcgt caatcagcgc
    10141 gctggcagag ttaatatcgt tatatgcagc atgacggata tctggtggca gtggcgcaaa
    10201 atcctgtgaa taggctggct gcccacccgc actgacagta aacagcgtgc gaccatgaaa
    10261 cgcattttta aacgccatga taccgctctt atggctgccg tagcggtcgt gagcgaattt
    10321 acgcgccagt tttagcgccg cttcgttggc ttccgcaccg gagttacaaa agaagacgcg
    10381 atcggcaaac gtggcgtcga tcaatttttt cgccagtcgc agtaccggct cgttggtgta
    10441 accgttgccg gtatgccaga acttactcgc ctgttcgttc agcgcttcac gcagttccgg
    10501 atgcgcatgg cccagcgcgt tcaccgcaat gccacccgcg aagtcgatat actctttccc
    10561 ctgctgatcc cacaagcgcg aaccttcgcc acgtaccggt ataaagggtg ccggagcgta
    10621 aacaggtatc atccattcat caaagttttc acgcgtaatt ggctgagaca tagcgacctc
    10681 tacagtaaat aattcgttat ttatatgtta acaataagta atgtttgcgc tgtaaatgta
    10741 gattgcaggg ttcgtgccag ccagtgataa aagtgcataa acggcggagg ctaactggaa
    10801 atcaaggagt tataaccaaa ccatatgcat ttaaagtgca tataaagtga atacgtttgc
    10861 gatgtgggtg aataaaaaga ataaaaaacg caatgttatg cagaagtaaa atataattct
    10921 ggaattgtga tcattgacga aatttactgg aaattactgc gccattctga cgcagcgcgc
    10981 accaaaagcg ggcatttttt gcgccatcgt tgacatcatt aacaaccatc gatcaaatca
    11041 cttaacaaca ggcggtaagc aacgcgaaat tctgctacca tccacgcact ctttatctga
    11101 ataaatggca gcgactatga aatttgtctc ttttaatatc aacggcctgc gcgccagacc
    11161 tcaccagctt gaagccatcg tcgaaaagca ccaaccggat gtgattggcc tgcaggagac
    11221 aaaagttcat gacgatatgt ttccgctcga agaggtggcg aagctcggct acaacgtgtt
    11281 ttatcacggg cagaaaggcc attatggcgt ggcgctgctg accaaagaga cgccgattgc
    11341 cgtgcgtcgc ggctttcccg gtgacgacga agaggcgcag cggcggatta ttatggcgga
    11401 aatcccctca ctgctgggta atgtcaccgt gatcaacggt tacttcccgc agggtgaaag
    11461 ccgcgaccat ccgataaaat tcccggcaaa agcgcagttt tatcagaatc tgcaaaacta
    11521 cctggaaacc gaactcaaac gtgataatcc ggtactgatt atgggcgata tgaatatcag
    11581 ccctacagat ctggatatcg gcattggcga agaaaaccgt aagcgctggc tgcgtaccgg
    11641 taaatgctct ttcctgccgg aagagcgcga atggatggac aggctgatga gctgggggtt
    11701 ggtcgatacc ttccgccatg ctaatccgca aacagcagat cgtttctcat ggtttgatta
    11761 ccgctcaaaa ggttttgacg ataaccgtgg tctgcgcatc gacctgctgc tcgccagcca
    11821 accgctggca gaatgttgcg tagaaaccgg catcgactat gaaattcgca gcatggaaaa
    11881 accgtccgat cacgcccccg tctgggcgac cttccgccgc taatttagca gctctcctgg
    11941 ctcaaactgg gtcaggagaa ttaaccttga gaaaaatcaa caaa
//
LOCUS       DAJVUJ010000129        11849 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.129, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000129 DAJVUJ010000000
VERSION     DAJVUJ010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11849
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.129"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            540..1196
                     /gene="pphA"
                     /locus_tag="OQQ48_003152"
     CDS             540..1196
                     /gene="pphA"
                     /locus_tag="OQQ48_003152"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HCS1953834.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVCAVRGNHEQMAMDALASQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLGERQKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
                     "
     gene            complement(1197..1388)
                     /locus_tag="OQQ48_003153"
     CDS             complement(1197..1388)
                     /locus_tag="OQQ48_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HCS1953835.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRQGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(1493..1729)
                     /locus_tag="OQQ48_003154"
     CDS             complement(1493..1729)
                     /locus_tag="OQQ48_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HCS1953836.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(1847..3286)
                     /gene="rsmF"
                     /locus_tag="OQQ48_003155"
     CDS             complement(1847..3286)
                     /gene="rsmF"
                     /locus_tag="OQQ48_003155"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HCS1953837.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAAGVGLNWDENLRLWQRDKELWLFPVDIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALATPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            complement(3366..5999)
                     /gene="yebT"
                     /locus_tag="OQQ48_003156"
     CDS             complement(3366..5999)
                     /gene="yebT"
                     /locus_tag="OQQ48_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HCS1953838.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RSNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGTGKEQDHFVALDTQPKYRLNNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDTGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVSGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISATGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            complement(5968..7251)
                     /gene="yebS"
                     /locus_tag="OQQ48_003157"
     CDS             complement(5968..7251)
                     /gene="yebS"
                     /locus_tag="OQQ48_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HCS1953839.1"
                     /translation="MALNTPQITPTKKITVRSIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            7381..7878
                     /gene="msrC"
                     /locus_tag="OQQ48_003158"
     CDS             7381..7878
                     /gene="msrC"
                     /locus_tag="OQQ48_003158"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HCS1953840.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            7975..8673
                     /gene="proQ"
                     /locus_tag="OQQ48_003159"
     CDS             7975..8673
                     /gene="proQ"
                     /locus_tag="OQQ48_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HCS1953841.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            8693..10741
                     /gene="prc"
                     /locus_tag="OQQ48_003160"
     CDS             8693..10741
                     /gene="prc"
                     /locus_tag="OQQ48_003160"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HCS1953842.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            10933..11814
                     /gene="htpX"
                     /locus_tag="OQQ48_003161"
     CDS             10933..11814
                     /gene="htpX"
                     /locus_tag="OQQ48_003161"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HCS1953843.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
BASE COUNT         3051 a         2917 c         3055 g         2826 t
ORIGIN      
        1 ctgcttaaaa aaagaacaaa tggtagtgtc cgctattgcc agtacacctc agtcgcaacc
       61 ccaaatcgta aaggaaaccg tagcacgtcg tatgcaagaa cgtgccacgg ctggctgatg
      121 gatgttcgat agcgcgagtt tgaatgaaaa tcagccggag atgattttac ataattgcta
      181 cggaattatt caatacagga attgcttgcg tctgcatgga ttgacctgaa attttcccga
      241 aaatttctct aaaaaactcg aaaaaaatgg taactggttg aatgtattaa tatgcaatgg
      301 tacgtgtcag ggattaaaag atgaacgtaa atttattcaa cgcattaatt ttaaagggtt
      361 ttattgtttg ttgacgaaaa caggaatcgt gttcggtctc tttttatctg ttaaaagcca
      421 gaagcatttc cttcgctgac tttatagtca accataacac acactctact gtctgagtcc
      481 agcgtttttt aacattcttg ttaagattat gtgatcttta gcacgggagg aaaatattga
      541 tgaaacagcc tgcgcccgtt tatcagagaa ttgcgggtca tcaatggcga catatctggc
      601 tttctggcga tatacacggt tgtcttgagc agttgcgccg caaattatgg cattgtcgtt
      661 ttgatccgtg gcgagattta cttatctcag tgggagacgt tatcgatcgt gggccgcaaa
      721 gtttacgttg tctgcagtta ctggaacaac attgggtttg tgcggtaaga ggcaatcatg
      781 aacagatggc gatggatgcg ctggcatccc agcagatgtc tttgtggttg atgaatggcg
      841 gcgactggtt tattgcgctg gcagataatc aacagaaaca agcgaaaacg gcgctggaaa
      901 aatgtcagca tttgcccttt attcttgaag tacacagtcg caccggcaag catgttattg
      961 ctcatgcgga ttatccagat gatgtttatg aatggcaaaa ggacgttgat ttgcatcagg
     1021 tcttgtggag ccgctcgcga ttaggtgaac gccaaaaagg gcagggaatt acaggtgctg
     1081 atcatttctg gtttggtcat acaccgttgc gacatcgcgt ggatattggc aacctgcatt
     1141 atattgatac cggtgctgtc tttgggggcg aactgactct tgtgcaattg caatgattaa
     1201 aaatcaccgt actcctgtgc aggtcgccag aaaccatcta taaaatcctc aatcggaaaa
     1261 caaccgccct ggcggatccg ttgatcgctc atagaataaa gacactgctg ttccgtgttg
     1321 tagacatcca caacaatatc ttcacaaccg ccatccaggt agcaaacaaa aagtaccagc
     1381 gcgaacattt catccccgaa gtgtggtgcc gtaccgttaa gtttaggaga gattttacaa
     1441 cgggggaata accaggacaa ataacccgcc atgccgacgg gttctttttg gatcaggcaa
     1501 gacgcataat ccaggcatca gattgttgat cgtaacgggt acggtaaagg acagagtgtt
     1561 cgccatttag cagggcaggg atactggttt cagcatccca ggcatcgagt tgcatacata
     1621 aatcggggtc agatttacaa ggaatggtta acgttcgatc acccggcgat tcaccgtgta
     1681 attcgccgtc gtcgatttca aaagcgccta tgcgcacact ggttttcatc ttcgtgctct
     1741 ccatttagct gccggttgtg gtacgaccag tccgatcgca ccatttttca gcctagttga
     1801 ggcagccgaa tgtgccagtc aaaaagtgcg ctttttttgt gcgctgtcag gcgttaccgg
     1861 taaaaagttt cccatcgcgc accagttcac gcggatagct gtttttcaat cgcgaaccaa
     1921 tccgtttggc taaaccaatc ggttgatgct ggaaagtaac caacacgtca tccgccactg
     1981 gcgcggcttg cgggtaaaca tcgcgcccgc gataccactc ctccgcttcc tgcggtgtca
     2041 gttcaaaagc gttcacattg tcgggggtgg caagggcaat aactgcttca tgctgccagc
     2101 gataaccttt gttgtgcgtt tcggcaagtt taatccccaa gcgagaaaat cggactttac
     2161 cgatcagggc ttcaatgtcc accgggaaca accacagttc tttgtcacgc tgccagaggc
     2221 gcaggttttc atcccagttt aagccaacac ctgcagccgc ctggcgaatt tgtccagctt
     2281 cgcgatcttt caccgggctg aacggaaaat taccgacttt gtatttgggg gcgggtaagg
     2341 cgggaatcgc ctgagtttta cgcagacgag caacgaagaa gccttcgcag tcgtaaattt
     2401 gtgggaaaac atgcaaaaag ccttcttcgg tcagcgcttt gtttgcacca gggaagagat
     2461 cgccaagtgg taaaaactct actgcgtcgg ggtaagtctc tttcagccac aggcaaacgg
     2521 cttcgttttc ttcctggttt aaggtacagg tcgagtaaac cagcgtacca ccaggacgta
     2581 atgcatgaaa ggcgctgtcg ataagctccc gttgtgtagc tgcgatttcc tgattgcttt
     2641 ctggtgacca gttttttagc gcatcgggat ctttacgcac cacgccttcg ccggagcagg
     2701 gagcgtccag caaaatggca tcaaacattt ctggcactgc cgcaccaaac acgcggccat
     2761 caaaatgtgt gagcgcaaca ttactgatgc cacagcggct gatattggca tgtaacactt
     2821 ttacccgact ggcggaaaac tcattggcaa ggattgcccc ttcgttattc atccgcgcgg
     2881 caatttgcgt cgtttttgag cctggcgcag cagcgacatc catcacccgc tgtggtgcat
     2941 taccgtcagc aaacaaggcg gcgacgggca acattgaact ggcttcctga atataaaaca
     3001 ggccacttaa atgctcggcg gtactaccca atggcaatgc atcttcattg tcgcgttcaa
     3061 tccagaaacc ttcttcacac cacggaattg gcgtaagcgt ccagccataa ggagcggtta
     3121 attgcaggaa atcagcaacg gagattttca gcgtattaac gcgaatgctg cggcgcaacg
     3181 ggcgctgaca ggcggcaaga aaatcatcaa atgagagcgt cgaaggcata gcttcgcgca
     3241 tttgtgtcag aaacgcgtcc gggaaataaa cggtgtgttg ggccacgagc atgtaccact
     3301 ggcaaaaaaa caggcgcgca gtttagcata aacgctccgg cgcaggcacg ccggagcagt
     3361 gggcattatt tgggaagcgc agttccccat tcacgccact ctttcggttc actttcctgc
     3421 aacaggaagt gtttgccttc ctgggctttc ggtgccagtg gcgtacccgg aggcgtggcg
     3481 aaggcgatgc cgccacggat aaactggtta aaggtgccgg ttttcactac gccgcccgtc
     3541 agaccaaagt ccagactgta acccgatgcc aaccagaaga cggaattgtt acgcaccagg
     3601 tgttgatagc gtttactgat gcgcatcgca atcatcacgc gatctgacaa tgtccccagc
     3661 gtcattccgg taaccgtacc tacttccaga ccacggaaca gcacaggcgt accaatacct
     3721 aacgaaccgg cttccggtgc ttcgacaata atgcttaagc catccaggta acgcgaatca
     3781 gtaatagtag cttcttgcaa ctcaaagtca cggcgaggat taccccgacc tggttcgaca
     3841 ttgatatacg gctggaggat agtatctaga tgctcaacgc ccgtggctga aatttgcggt
     3901 gtgaccaccg agaagcgcgt accgccgcgg gcgaaggtct ggacatactc cggatagagc
     3961 accgcctttg cctgtacttc attgcgcgcg gtaatcagat ccagcgtctg gatttgcccg
     4021 atatcaatac cgagatagcg aattggcatc ccgaccgcca gttttccggc atcgaaagcg
     4081 tgaagcgtaa tctgcccact aaccgcacgg gccgctgttt cggaagcata cagaattcgt
     4141 ttgtcgcctt tacgctgact ggcgctggca ccgctgaggt tatcgaagct aatggctccc
     4201 tttaatgctc tggagagcgg ggatgcctgt acggtcaggc cactaccatt cagctgaact
     4261 ttcgccccgc cttctgccca gaacacgcta ttgctggtca gaaggttgcg atactccggc
     4321 ttaatatgca gatcgatatc aaacgcgtta gctcgcggac gcacggtaat cacttcacca
     4381 acttcaaatt tacggtacag cactaccgat cctgcctgca catccggcag cgtctctgca
     4441 ctcaaactca cggttgtggt gggtaaatcg ctaaggctgt tctccagcgc tttttccaga
     4501 ttggcataca gtggatagct ggctttcatc tcacctttat cgcctggcag aatacgtatc
     4561 ccgccgttaa tccattctga ggcgctggca ccaagaaact caacgccatc cagccccacc
     4621 ttcacgtcga cacggctgtt gacgacaaat ttgctatcgc cttttaccag ttcgcgatgc
     4681 tgaggctcga tggcgacggt aaaggtaacg cctttgctgg tgagtttacg atcaataacc
     4741 tggcctacct gcacgccgtg aagaatgagc ggctgacccg tatcaatacc gtaactttcc
     4801 ggcgcggtca gggtcagcgt cagaacatca ggttcatgca gcagtgcttt ttcgcctggc
     4861 acaacaacga actctttgcg tggctcgcca tcgccgggta ccaactcgaa ggttttgcca
     4921 gtcagcaggg cgctgagatt ggcatcgcta agggataatt tcggattacg taattcgatg
     4981 cgggtatttt cacgaagcag ggtaacgacg ctgggatcaa ctgtcatttc cccagtgact
     5041 ttaccaccag gatttaaatc cagtttagtt agctgtccga cttccagtcc ctgatacatt
     5101 aatggcgtcg agtcggcggt taatccggcc ccactcggca gttccagttt gattattacg
     5161 ccacgctggc tatgggcgag gtcttcatac agaccaaagg tatcttccgc ctcggcaggt
     5221 ttcgactctt ctggtgaatc gaaggcaatc gcaccgttaa ccagtgccgc caggctttcc
     5281 agtttcacct tcgcgccact gatactgaca ttggcatcaa cgccggaaac gttccagaaa
     5341 cggctacctt ttttcaccag atcggtaaaa cgccgctcga tcaggacatc aatcaccacg
     5401 ccttgtttgt tgggattgat ggcatagtcg tagacttttc ccaccgggat cttgcggaaa
     5461 tagaccaatg aaccgctgtt cagcgaaccg agatcggggg cttgcaggtg gatcatcaga
     5521 tcgccattgt tcagccgata tttcggttgg gtatcgagtg cgacaaagtg atcctgctct
     5581 ttacctgtac ccggcatcat gccgatatag ttcccaccga cgagggcgtc cagcccggag
     5641 acacctgcca acgatgcttt tggcgtcacc agccagaatt gagtctcttc gcgcagcgca
     5701 tctttcatat cggacttgat gctgaccttg acttcaatct tacgaagatc gtcgctgagg
     5761 ctgatatcct gcactgttcc gacttcaacg ccctgataac gaacaggcgt acggcccggg
     5821 acaataccat ccgccgacat aaagtcgatg gtgacggtat tacttcggtc ctgataactg
     5881 tcccaaatca gccaaccggc aatcattagc gcgatgaaag gcagcagcca gaaaggtgag
     5941 atacggcgtt tatttttaat ctgcgcttca gtcgtcgaag cgggcgtttc ctgactcatg
     6001 tgcatcccaa agtaagcggc tgtccagcca ttccacagca agaatagtca aaattacggc
     6061 tgcgccaaaa taaaacgcag ccggtcccat agtaaaagcg aggatctgat cgcgattaat
     6121 cagcgacatg gttaaagata tgacaaacag gtcgagcatc gaccagcgtc caatccaggt
     6181 caccatccgc agtaacagaa tgcgtgtgcg taaaccttgc tggcatttaa aatgaatgct
     6241 caacagtaaa gtgaacatga cgatcacttt agtaaacggt accagaatac tggcgataaa
     6301 cacgattccg gcgaccgcaa tgttgctact tgccagcgac ataatcccgg aaagaatcgt
     6361 atcttcctgc cggccaccat tcagataaat gatagaaata ggcaataggt tggctggtaa
     6421 caacaaaacg attgacgcta acagcgccgc ccagcatttt tgcaaactat gacgacgacg
     6481 caggcgtagc gggatatggc aacgcgggca gcgaccacgc tgatcaggat agccggtaaa
     6541 atggcaccca agacagacac gaagtttctc gtccctacgc gtagcggggc gctgcggata
     6601 atatcgctcc cacagctcct caacattaag atgtgacaac gtcaccgtcg ttaaaatcac
     6661 caacgccaca aaagagaaca agccgacacc cgcctggata tgggcataat cctgtacctt
     6721 tatagaagca acgccaatgc cgaccaggta gatatccagc attacccact ctttaagacg
     6781 ctcaagcatc agcagtaccg gacgtaaatt cattcccagt cggttaccaa accataaata
     6841 tgctatggag gacaccagaa tgaggggggc accaataacg cagaaaaaga ccatcgcccc
     6901 cgttatcgta tcgccctgtt tggtcatttg ccagatgcct tgcatgacgt ttgcgtcgat
     6961 acgaatacct aacagccaga tatgcaacag tggttcgccc caggcaaacg gcatcagcaa
     7021 cagcatggtg aaagccattg ccgccaggcg cgttagcgac cagtcgcgcc cgtcgcgaat
     7081 ttttgcctga cagcgcggac aataggcact ctgatgagaa tttatctcgg gcaggctaaa
     7141 cagcatgtca cattgcgggc aacgttggta atcaccacgc ggcagttcct cgccgattga
     7201 cctcactgtt atcttttttg tcggcgtaat ttgtggtgtg ttaagagcca tgacgtgcca
     7261 aatgtaataa gaattaacat taatcttaac ccatgatggg aatgatcttg agcattaacg
     7321 catttaatgc ttattttaat aggctggcag ttccaggtaa gacaaacaag tgattatata
     7381 atgaacaaaa cagaatttta cgcggattta aatcgcgact ttaacgcgct gatggcggga
     7441 gaaaccagtt ttctggcaac gcttgcgaac accagtgcgt tgttatacga gcgtctcact
     7501 gacgtaaact gggcaggttt ttatttgctt gaggacgata cactggtact cggaccattt
     7561 cagggcaaaa ttgcctgtgt ccggataccc gtcgggcgcg gggtgtgcgg cactgcggtt
     7621 gcccgcaatc aagtgcagcg tatcgaggat gttcacgcgt ttgacgggca tattgcctgt
     7681 gatgcggcga gtaattctga aattgttctg ccgctggtgg tgaaaaatca gattattggt
     7741 gttcttgaca tcgatagtac cgtcttcggt cgctttacag acgaggacga gcaaggctta
     7801 cgtcagcttg tggcacagct tgaaaaagtg cttgcaacga cggattacaa aaaattcttt
     7861 gcgagcgtcg caggataatc aacggataac gtagcattta ctggtagcgt cattataatg
     7921 acgcctgttc atgcctgcgc ttgttggcta cgtccgttgt aatcaggaaa tttcatggaa
     7981 aatcaaccta agttgaatag cagtaaagaa gtaatcgcgt ttctggccga acgttttccc
     8041 cactgtttca gtgcggaagg tgaagcgcgt ccgctgaaaa tcggtatttt tcaggatttg
     8101 gtcgatcgtg ttgccgggga aatgaacctg agcaaaacgc aattacgatc cgctttacgt
     8161 ctctacactt cgagctggcg ttatctttac ggtgttaaac ccggcgcaac gcgtgtcgat
     8221 cttgacggca acccatgcgg tgagctggac gagcaacatg tagagcatgc tcgcaagcag
     8281 cttgaagaag cgaaagcgcg tgttcaggca cagcgtgctg aacagcaggc gaaaaaacgc
     8341 gaagctgccg cagctgctgg tgagaaagaa gacgcaccgc gccgcgaacg caagccacgt
     8401 ccgaccacgc cacgccgcaa agaaggcgct gaacgtaaac ctcgtgcgca aaagccggta
     8461 gagaaagcgc caaaaacagt aaaagcacct cgcgaagaac agcacacccc ggtttctgac
     8521 atttcagctc tgactgtcgg acaagctctg aaggtgaaag cgggtcaaaa cgcgatggat
     8581 gccaccgtat tagaaatcac caaagacggc gtccgcgtcc agctgaattc gggtatgtct
     8641 ttgattgtgc gcgcagaaca cctggtgttc tgaaacggag gccgggccag gcatgaacat
     8701 gttttttagg cttaccgcgt tagctggcct gcttgcaata gcaggccaga ccttcgctgt
     8761 agaagatatc acgcgtgctg atcaaattcc ggtattaaag gaagagacgc agcatgcgac
     8821 ggtaagtgag cgcgtaacgt cgcgcttcac ccgttctcat tatcgccagt tcgacctcga
     8881 tcaggcattt tcggccaaaa tctttgaccg ctacctgaat ctgctcgatt acagccacaa
     8941 cgtgctgctg gcaagcgatg ttgaacagtt cgcgaaaaag aaaaccgagt taggcgatga
     9001 actgcgttca ggcaaactcg acgttttcta cgatctctac aatctggcgc aaaagcgccg
     9061 ttttgagcgt taccagtacg ctttgtcggt actggaaaag ccgatggatt tcaccggcaa
     9121 cgacacttat aaccttgacc gcagcaaagc gccctggccg aaaaacgagg ctgagttgaa
     9181 cgcgctgtgg gacagtaaag tcaaattcga cgagttaagc ctgaagctgg caggaaaaac
     9241 ggataaagaa attcgtgaaa ccctgacacg ccgctacaaa tttgccattc gtcgtctggc
     9301 gcaaaccaac agcgaagatg ttttctcgct ggcaatgacg gcgtttgcgc gtgaaatcga
     9361 cccgcatacc aactatcttt ccccgcgtaa taccgaacag ttcaacactg aaatgagttt
     9421 gtcgctggaa ggtattggcg cagtgctgca aatggatgat gactacaccg ttatcaattc
     9481 gatggtggca ggtggtccgg cagcgaagag taaagctatc agcgttggtg acaaaattgt
     9541 cggtgttggt caaacaggca agccgatggt tgacgtgatt ggctggcgtc ttgatgatgt
     9601 ggttgcctta attaaagggc cgaagggcag taaagttcgt ctggaaattt tacctgctgg
     9661 taaagggacc aagacccgca ctgtaacatt gacccgtgaa cgtattcgtc tcgaagaccg
     9721 cgcggttaaa atgtcggtga agaccgtcgg taaagagaaa gtcggcgtgc tggatattcc
     9781 gggcttctat gtgggtttga cagacgatgt caaagttcaa ctgcagaaac tggaaaaaca
     9841 gaatgtcagc agtgtgatca tcgacctgcg tagcaatggc ggtggggcgt tgactgaagc
     9901 ggtatcgctc tcaggtctgt ttattcctgc gggtcccatt gttcaggtcc gcgataacaa
     9961 cggcaaggtt cgtgaagaca gcgataccga cgggcaggtg ttctataaag gcccgctggt
    10021 ggtactggtt gaccgtttca gtgcttcggc ttcagaaatc tttgccgcgg caatgcagga
    10081 ttacggtcgt gcgctggttg tgggtgaacc gacgtttggt aaaggcaccg ttcagcaata
    10141 ccgttcattg aaccgtattt acgatcagat gttacgtcct gaatggccag cgctgggttc
    10201 tgtgcagtac acgatccaga aattctatcg cgttaacggc ggcagtacgc aacgtaaagg
    10261 cgtaacgcca gacatcatca tgccgacggg taatgaagaa acggaaacgg gtgagaaatt
    10321 cgaagataac gcgctgccgt gggatagcat tgatgccgcg acttatgtga aatcaggcga
    10381 tttaacggcc tttgggccgg agctgctgaa ggaacataat gcgcgtatcg cgaaagatcc
    10441 tgagttccag aacatcatga aggatatcgc gcgcttcaac gctatgaagg acaagcgcaa
    10501 tatcgtttct ctgaactacg ctgtgcgtga gaaagagaat aatgaagatg atgcgacgcg
    10561 tctggcgcgt ttgaacgaac gctttaaacg cgaaggtaaa ccggagttga agaagctgga
    10621 tgatctaccg aaagattacc aagagccgga tccttatctg gatgagacgg tgaatatcgc
    10681 actcgatctg gcgaagcttg aaaaagccag acccgcggaa caacccgctc ccgtcaagta
    10741 atatcaatca ggcacaagaa attgtgcctg attttttaac agcggcaaga tgccgtaaat
    10801 cagatgctac aaaatgtaaa gttgtgtctt tctggtgact tacgcactat ccagacttga
    10861 aaatagtcgc gtaacccata cgatgtgggt atcgcatatt gcgttttgtt aaactgaggt
    10921 aaaaagaaaa ttatgatgcg aatcgcgctc ttcctgctaa cgaacctggc cgtaatggtc
    10981 gttttcgggc tggtactgag cctgacaggg atacagtcga gcagcgttca ggggctgatg
    11041 atcatggcct tgctgttcgg ttttggtggt tccttcgttt cgcttctgat gtccaaatgg
    11101 atggcattac gatctgttgg cggggaagtg atcgagcaac cgcgtaacga aagggaacgt
    11161 tggctggtca atactgtagc aacccaggct cgtcaggcag ggatcgctat gccgcaagtg
    11221 gctatctacc atgcgccgga catcaacgct tttgcaaccg gtgcgcgccg tgatgcctct
    11281 ctggttgctg tcagcaccgg tttgctgcag aacatgagcc cggatgaagc cgaggcggta
    11341 attgctcacg aaatcagcca catcgccaat ggtgatatgg tcaccatgac gctgattcag
    11401 ggcgtggtga acaccttcgt tatctttatt tcccgtattc tggcgcagct tgccgcgggt
    11461 tttatgggcg gaaatcgtga tgaaggtgaa gagagcaacg gcaacccgct gatctacttt
    11521 gcggttgcaa cggttctgga actggtgttt ggtattctgg cgagcattat caccatgtgg
    11581 ttctcgcgtc atcgtgaatt ccatgctgat gccggttcgg caaaactggt tggtcgcgag
    11641 aaaatgattg ccgcgctgca gcgcctgaaa accagctatg aaccgcaaga agcaaccagc
    11701 atgatggctt tctgcattaa tggtaagtcg aaatcgctca gtgagttgtt catgactcac
    11761 ccgccgctgg ataaacgaat tgaagctctg cgtacgggtg aatacctgaa gtaatatgaa
    11821 gtaaatacta aaaagtgcgt cctgatcga
//
LOCUS       DAJVUJ010000130        11775 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.130, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000130 DAJVUJ010000000
VERSION     DAJVUJ010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11775
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.130"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(10..660)
                     /gene="hxpA"
                     /locus_tag="OQQ48_003162"
     CDS             complement(10..660)
                     /gene="hxpA"
                     /locus_tag="OQQ48_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HCS1953844.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTCLEHIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     AEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQLNGDVIIQ"
     gene            complement(671..1165)
                     /locus_tag="OQQ48_003163"
     CDS             complement(671..1165)
                     /locus_tag="OQQ48_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HCS1953845.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACLRQYHLSANEINQ
                     IINA"
     gene            complement(1249..1704)
                     /locus_tag="OQQ48_003164"
     CDS             complement(1249..1704)
                     /locus_tag="OQQ48_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HCS1953846.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            2042..3244
                     /gene="ackA"
                     /locus_tag="OQQ48_003165"
     CDS             2042..3244
                     /gene="ackA"
                     /locus_tag="OQQ48_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCS1953847.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            3319..5463
                     /gene="pta"
                     /locus_tag="OQQ48_003166"
     CDS             3319..5463
                     /gene="pta"
                     /locus_tag="OQQ48_003166"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCS1953848.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNQEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            5653..7173
                     /gene="yfcC"
                     /locus_tag="OQQ48_003167"
     CDS             5653..7173
                     /gene="yfcC"
                     /locus_tag="OQQ48_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCS1953849.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATMIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQITVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(7206..7748)
                     /gene="yfcD"
                     /locus_tag="OQQ48_003168"
     CDS             complement(7206..7748)
                     /gene="yfcD"
                     /locus_tag="OQQ48_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HCS1953850.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(7806..8357)
                     /gene="yfcE"
                     /locus_tag="OQQ48_003169"
     CDS             complement(7806..8357)
                     /gene="yfcE"
                     /locus_tag="OQQ48_003169"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HCS1953851.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(8413..9045)
                     /gene="yfcF"
                     /locus_tag="OQQ48_003170"
     CDS             complement(8413..9045)
                     /gene="yfcF"
                     /locus_tag="OQQ48_003170"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HCS1953852.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASVAMAEHLLALGQPNLFGEW
                     CIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            9181..9828
                     /gene="yfcG"
                     /locus_tag="OQQ48_003171"
     CDS             9181..9828
                     /gene="yfcG"
                     /locus_tag="OQQ48_003171"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HCS1953853.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGELGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLTMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            9885..10247
                     /gene="folX"
                     /locus_tag="OQQ48_003172"
     CDS             9885..10247
                     /gene="folX"
                     /locus_tag="OQQ48_003172"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HCS1953854.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            10268..11161
                     /locus_tag="OQQ48_003173"
     CDS             10268..11161
                     /locus_tag="OQQ48_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HCS1953855.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIDSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPATAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(11209..>11775)
                     /gene="rpnB"
                     /locus_tag="OQQ48_003174"
     CDS             complement(11209..>11775)
                     /gene="rpnB"
                     /locus_tag="OQQ48_003174"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HCS1953856.1"
                     /translation="AGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSS
                     AFPLVDITVVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLK
                     ALFNYVLQTGDAQRFRAFIGEIAERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQ
                     EMLDRGLDRELVMMVTRLSPDDLIAQSH"
BASE COUNT         2818 a         3122 c         2944 g         2891 t
ORIGIN      
        1 tcaggtgttt cactgaataa taacatcgcc atttagctgt ttggtcacag taatttgctc
       61 cagactgtgg aggaccaaat cgacctcatt caggcgcggg gtatctgccg gagcgttaac
      121 cgcaatgaca tggcaacccg ccgccaggcc agaaagcacg ccagcgggag catcttccgc
      181 caccacacac tcctgcggcg caagccccag cagctgcgca cctaacagat acgcatcagg
      241 ttctggtttt ccgcgcttca ctcgctcagc ggttacaaac acctctggtg cgggaagccc
      301 agctatttta tggcgcgctc gcgctaccgg catggagcca gaagtcacaa tggcccacgg
      361 aatacctgct ttattcaaat gattgagtaa ggcgattgcc cccggaagcg cggtaatacc
      421 ttcggtttcc gtggcctcga tgtgctccag acacgtaaac tcggcggcaa tatcagcctc
      481 ggatttgccc gccataaaat ggcgcaaaga ggtgatcgcc tgtttaccgt gaatgaaagc
      541 cagcacctct tccggcgcta acccatgacg tctggcccag ttgctccacg cccgttctac
      601 cgcaggcagg gaatccacca gcgttccatc aagatcaaac agaaaacctt tgcaccgcac
      661 gcgggcctcc tcaggcgttg ataatttgat taatttcgtt ggcgctcaaa tggtactgac
      721 gcaggcaggc atgccacaca ttaagcatgc gctgatattt ttcccacatt ggcgtctggg
      781 cgttaaaacc atgagtccca gcgtcaaaat gggtatagcg cccttccaca ttaaccataa
      841 agcggacata accgaggtaa cgtgcttcag tggcggcgtc aaagccgagg aaggtgacac
      901 gacgttcatc gatggattgc tggtcctgca aattagacca ggaaacatgc aacgcatgat
      961 acatctccat aatgtcgatg atagtgcggc aggtttcttc tttcagctcg ccaaactcgc
     1021 gatccaattc acgcatctgt aatccgtaac cacgctcaat aattgtttgc aggcgacggt
     1081 aacgttcagc atttgccgga tcgagcatag tcatcatctt gtactgatta gacaaaataa
     1141 gacgttgcgc gttggtcatt tccattgttg actcctgtat cactctacta cggtgaaaaa
     1201 aaaagaaggc tgagtatgcc ttcttttata tgcgtaatca ggggtcaatt acaaatcatc
     1261 aaggaaagtt ttatccagtt gtttgaaggc gcgcttaagc gtgtcagcta atgcctggta
     1321 atcaggcttg ccttcaacgg gtgccaacac ctgtccagac tcctgcaatt taccgcgaac
     1381 ttcataaaac cagttgagga tagcaggggg taatggcgtt acagaacgct tgcccagcca
     1441 ccataatccc tgcatgggta aacttaaggc gaacagcgca gtggcaactg ccggcccaag
     1501 ctgaccgccc agggcaatct gccagcagag agtaaatacg gcgatcggcg gcataaaacg
     1561 gatcgcataa cgcgtcatct tgataacgcg attttcgaca aagaccgggg caaggcgttt
     1621 ttccagcggc cacgtctttg agtaatgctg tccccggcga aacaagctaa aaaaattaac
     1681 agaacgatta tccggcgttg acatgcttca cctcaacttc acatataaag attcaaaaat
     1741 ttgtgcaaat tcacaactca gcgggacaac attcaaaaca ttttgtcttc catacccact
     1801 atcaggtatc ctttagcagc ctgaaggcct aagtagtaca tattcattga gtcgtcaaat
     1861 tcatatacat tatgccattg gctgaaaatt acgcaaaatg gcatagactc aagatatttc
     1921 ttccatcatg caaaaaaaat ttgcagtgca tgatgttaat cataaatgtc ggtgtcatca
     1981 tgcgctacgc tctatggctc cctgacgttt ttttagccac gtatcaatta taggtacttc
     2041 catgtcgagt aagttagtac tggttctgaa ctgcggtagt tcttcactga aatttgccat
     2101 catcgatgca gtaaatggtg aagagtacct ttctggttta gccgaatgtt tccacctgcc
     2161 cgaagcacgt atcaaatgga aaatggacgg caataaacag gaagcggctt taggtgcagg
     2221 cgccgctcac agcgaagcgc tcaactttat cgttaatact attctggcac aaaaaccaga
     2281 actgtctgcg cagctgactg ctatcggtca ccgtatcgta cacggcggcg aaaagtatac
     2341 cagctccgta gtgatcgatg agtctgttat tcagggtatc aaagatgcag cttcttttgc
     2401 accgctacac aacccggctc acctgatcgg tatcgaagaa gctctgaaat ctttcccaca
     2461 gctgaaagac aaaaacgttg ctgtatttga caccgcgttc caccagacta tgccggaaga
     2521 gtcttacctc tacgccctgc cttacaacct gtacaaagag cacggcatcc gtcgttacgg
     2581 cgcgcacggc accagccact tctatgtaac ccaggaagcg gcaaaaatgc tgaacaaacc
     2641 ggtagaagaa ctgaacatca tcacctgcca cctgggcaac ggtggttccg tttctgctat
     2701 ccgcaacggt aaatgcgttg acacctctat gggcctgacc ccgctggaag gtctggtcat
     2761 gggtacccgt tctggtgata tcgatccggc gatcatcttc cacctgcacg acaccctggg
     2821 catgagcgtt gacgcaatca acaaactgct gaccaaagag tctggcctgc tgggtctgac
     2881 cgaagtgacc agcgactgcc gctatgttga agacaactac gcgacgaaag aagacgcgaa
     2941 gcgcgcaatg gacgtttact gccaccgcct ggcgaaatac atcggtgcct acactgcgct
     3001 gatggatggt cgtctggacg ctgttgtatt cactggtggt atcggtgaaa atgccgcgat
     3061 ggttcgtgaa ctgtctctgg gcaaactggg cgtgctgggc tttgaagttg atcatgaacg
     3121 caacctggct gcacgtttcg gcaaatctgg tttcatcaac aaagaaggta cccgtcctgc
     3181 ggtggttatc ccaaccaacg aagaactggt tatcgcgcaa gacgcgagcc gcctgactgc
     3241 ctgatttcac accgccagct cagctggcgg tgctgttttg taacccgcca aatcggcggt
     3301 aacgaaagag gataaaccgt gtcccgtatt attatgctga tccctaccgg aaccagcgtc
     3361 ggtctgacca gcgtcagcct tggcgtgatc cgtgcaatgg aacgcaaagg cgttcgtctg
     3421 agcgttttca aacctatcgc tcagccgcgt accggtggcg atgcgcccga tcagactacg
     3481 actatcgtgc gtgcgaactc ttccaccacg acggccgctg aaccgctgaa aatgagctac
     3541 gttgaaggtc tgctttccag caatcagaaa gatgtgctga tggaagagat cgtcgcaaac
     3601 taccacgcta acaccaaaga cgctgaagtc gttctggttg aaggtctggt cccgacacgt
     3661 aagcaccagt ttgcccagtc tctgaactac gaaatcgcta aaacgctgaa tgcggaaatc
     3721 gtcttcgtta tgtctcaggg cactgacacc ccggaacagc tgaaagagcg tatcgaactg
     3781 acccgcaaca gcttcggcgg tgccaaaaac accaacatca ccggcgttat cgttaacaaa
     3841 ctgaacgcac cggttgatga acagggtcgt actcgcccgg atctgtccga gattttcgac
     3901 gactcttcca aagctaaagt aaacaatgtt gatccggcga agctgcaaga atccagcccg
     3961 ctgccggttc tcggcgctgt gccgtggagc tttgacctga tcgcgactcg tgcgatcgat
     4021 atggctcgcc acctgaatgc gaccatcatc aacgaaggcg acatcaatac tcgccgcgtt
     4081 aaatccgtca ctttctgcgc acgcagcatt ccgcacatgc tggagcactt ccgtgccggt
     4141 tctctgctgg tgacttccgc agaccgtcct gacgtgctgg tcgccgcttg cctggcagcc
     4201 atgaacggcg tagaaatcgg tgccctgctg ctgactggcg gctacgaaat ggacgcgcgc
     4261 atttctaaac tgtgcgaacg tgctttcgct accggcctgc cggtatttat ggtgaacacc
     4321 aacacctggc agacctctct gagcctgcag agcttcaacc aggaagttcc ggttgacgat
     4381 cacgaacgta tcgagaaagt tcaggaatac gttgctaact acatcaacgc tgactggatc
     4441 gaatctctga ctgccacttc tgagcgcagc cgtcgtctgt ctccgcctgc gttccgttat
     4501 cagctgactg aacttgcgcg caaagcgggc aaacgtatcg tactgccgga aggtgacgaa
     4561 ccgcgtaccg ttaaagcagc cgctatctgt gctgaacgtg gtatcgcaac ttgcgtactg
     4621 ctgggtaatc cggcagagat caaccgtgtt gcagcgtctc agggtgtaga actgggtgca
     4681 gggattgaaa tcgttgatcc agaagtggtt cgcgaaagct atgttggtcg tctggtcgaa
     4741 ctgcgtaaga acaaaggcat gaccgaaacc gttgcccgcg aacagctgga agacaacgtg
     4801 gtgctcggta cgctgatgct ggaacaggat gaagttgatg gtctggtttc cggtgctgtt
     4861 cacactaccg caaacaccat ccgtccgccg ctgcagctga tcaaaactgc accgggcagc
     4921 tccctggtat cttccgtgtt cttcatgctg ctgccggaac aggtttacgt ttacggtgac
     4981 tgtgcgatca acccggatcc gaccgctgaa cagctggcag aaatcgcgat tcagtccgct
     5041 gattccgctg cggccttcgg tatcgaaccg cgcgttgcta tgctctccta ctccaccggt
     5101 acttctggtg caggtagcga cgtagaaaaa gttcgcgaag caactcgtct ggcgcaggaa
     5161 aaacgtcctg acctgatgat cgacggtccg ctgcagtacg acgctgcggt aatggctgac
     5221 gttgcgaaat ccaaagcgcc gaactctccg gttgcaggtc gcgctaccgt gtttatcttc
     5281 ccggatctga acaccggtaa caccacctac aaagcggtac agcgttctgc cgacctgatc
     5341 tccatcgggc cgatgctgca gggtatgcgc aagccggtta acgacctgtc ccgtggcgca
     5401 ctggttgacg atatcgtcta caccatcgcg ctgactgcga ttcagtctgc acagcagcag
     5461 taatctcgtc atcatccgca gctttgcgct gcggatatct gaaccggaaa taatcactat
     5521 ttccggtttt ttattctctt aatctgcatt aatcctttct gattatcttg cttaactgcg
     5581 ctgcatcaat gaattgcgcc atttcacttt gcatacttac cactttgttt tgtgcaaggg
     5641 aatatttgcg ctatgtccgc aatcactgaa tccaaaccaa caagaagatg ggcaatgccc
     5701 gatacgttgg tgattatctt ttttgttgct attttaacca gccttgccac ctgggtagtt
     5761 ccggtgggga tgtttgacag tcaggaagtg cagtatcagg ttgatggtca aacaaaaaca
     5821 cgcaaagtcg tagatccaca ctcatttcgc attctgacta acgaagcagg cgaacctgag
     5881 tatcaccgcg tacagctgtt cacgacgggc gatgaacgcc cgggcctgat gaacttcccg
     5941 tttgaaggat taacctcagg atcgaaatac gggacagctg ttggcatcat catgtttatg
     6001 ctggtgattg gcggcgcgtt tggcattgtg atgcgtacag gaaccattga taacggtatc
     6061 ctggcgctta ttcgccatac ccgcgggaat gaaattctct ttattcctgc gctgtttatt
     6121 ctgttttcac ttggcggcgc ggtatttggt atgggagaag aggccgtcgc ctttgccatt
     6181 atcatcgcac cgctaatggt ccggctgggc tatgacagta ttaccaccgt cctggtgacc
     6241 tatattgcca cgcaaatcgg ttttgccagt tcgtggatga acccgttttg tgtggtcgtt
     6301 gctcagggga ttgccggcgt tccggtgctt tctggctccg ggttgcgcat cgtggtgtgg
     6361 gttatcgcca ctatgattgg cctgatcttt accatggtgt acgcctcacg agtgaaaaag
     6421 aatcctcttc tgtcacgcgt gcatgagtcc gaccgcttct ttcgtgaaaa gcaggcggat
     6481 gttgaacaac gtccgtttac ctttggtgac tggctggtat tgattgtcct gaccgccgta
     6541 atggtctggg tgatttgggg cgtgatcgtt aatgcctggt ttattccaga aattgccagc
     6601 cagttcttca ccatgggtct ggtgattggc atcatcggcg ttgttttccg ccttaacggc
     6661 atgacggtta ataccatggc ttcatccttt accgaagggg cgcgaatgat gatcgcccct
     6721 gccctgctgg tgggtttcgc caaagggatt ttgctgctgg tcggtaatgg tgaagcgggt
     6781 gatgccagcg tgttaaatac catcctcaac agcattgcca atgccattag cggtctggac
     6841 aacgcggtcg cggcctggtt tatgttgctc ttccaggcgg tatttaattt cttcgtgacg
     6901 tccggttctg gtcaggcggc gttaaccatg ccgttactgg caccgcttgg cgatctggtc
     6961 ggtgttaacc gtcagattac cgtgctggca ttccagtttg gtgatggctt cagtcacatc
     7021 atttacccaa cctcagcttc gttaatggca actctcggtg tgtgcagggt ggacttccgt
     7081 aactggctga aggtgggcgc gaccctactt ggactgctgt ttattatgtc cagcgtcgta
     7141 gtgatcggcg ctcagttgat gggctaccac taaaaatgtt aagagccgca attgcggctc
     7201 ttttttcatt ctgccgtttc agtctctacg gcttcatttt tggcattgcg cttcatccac
     7261 aacgccagcg ctttcagcga gtctggagtg aactcatcgc agcgtgcggt gatttcttcc
     7321 ggcgtcagcc agcaaacttc actgacttca tcttcctgta gggcgaaggg accgtgagag
     7381 acgcagctga acaacgcgcc ccagacacgg caatttttat cttcgaaata gaactgcccg
     7441 tgctcggcaa aggggacacc ggcaatgccc aactcttctt ccgcttcgcg acgcgcggat
     7501 tccagcagtt gctcatcggc ctgaaccaca ccgcctgcgg tcgcatctaa catgccgggt
     7561 aaaaagtctt ttgtctcggt acgacgctgg accagaattt tgcccatgcc atcatgcacg
     7621 acgatgtaag ttgcacgatg acgcagacac tgtgcccgca tttgttcccg gctggcttgt
     7681 gcaatgactt cgttctcttc attgacaata tccacccatt cagtacttgc caaacgacgc
     7741 tgttccatta tcaggaaacc ttctttttct ggcgctctta cggcgcattt tgagttgtgg
     7801 gtaaattacg gattaatcgc gacctgcgca atgatacttt gatcattgag tgcgattaca
     7861 ctaagtacgt cattatccag catgccataa ctcgccggat taccgccttt cggaatactc
     7921 accgagccgg ggttgaagtg gaaaatctcc ccccgctgtt ctgccactgg cagatgggta
     7981 tgaccgtaca ccagcacatc gttctggttt aaagcaggta gattttccgg gccaaaaaga
     8041 tgaccatgcg tcaaaaacag acgttgtttt tccagtaata cctgttgcca cggcgcggtt
     8101 atcgggaaat gcagcagcat ttgatccact tcgctgtcgc agttgccgcg cacagcgata
     8161 accttatgtg ccacttcatt aagccgttca gcgactttgg ctggcgcgta accctccggt
     8221 aaagcattac gcgggccgtg attcaacacg tcgccaagga tcaccagcca ctgggcaccg
     8281 ctttgggcaa acaactccag aacacgttcc gtcgccggta acgacccatg aatgtccgat
     8341 gcaaacatca gtttcatcac tcactcctcg tcgaaaaaac gtaagctatg atactggatt
     8401 ctgtcgttgc tatcagccag attgcttcgc cgaaagtgca ataaaacgct ggacagacgc
     8461 tcgctgccac tggaatgtcg cataatccac caggcgttct ggcacctcat cgccatgtag
     8521 taccaggcgg ttaatcatta gcgccagatc agtatcagca atgcaccatt caccaaataa
     8581 attcggctga cccagtgcta acaaatgttc tgccatcgcg acactggctt ttccctcggc
     8641 cgttagtggc gcttttttcg cccccgcaaa gacaacatcc gtcggacgct cttcgcggat
     8701 gggcatcaga tcgctgcgca gccaggcctg aatctgtcgt gcacgcgcac gattttctaa
     8761 atcaagcgga taaatacgtt cccaggtcgg tggcgcaaat cgatcttcca gatactccgc
     8821 aatggcagaa gattcactca actcaaaatc atcgatttgt aataacggca cacggcgtgt
     8881 ctgaccgtaa ccttgccacg tcggctgcaa atgttcaccg ctgtcgaggt cgatggtctt
     8941 gatatgaaac gacaggcctt tttcctgcaa cgccacccaa gcggataaca cataagggga
     9001 gaaaaagtgg gcatctgacc aaagcgtgat agcgggttta ctcattatgt cctcggcaat
     9061 aaaggaggct ggctatcaaa ctatagagcc tttttgtcac tgtcacgtga cgaatactca
     9121 cgtaagtcgc cgcgctttct atacttgtgg tgttcagatg attaaacaac cggagctgca
     9181 atgatcgatc tctatttcgc cccgacaccc aatggtcaca aaattacgct gtttctcgaa
     9241 gaagcagagc ttgattatcg cttgattaag gtagacctgg ggaaaggcgg tcagtttcgt
     9301 ccggaatttt tgcgcatttc gcccaacaac aaaattccgg caattgttga tcattctcct
     9361 gccgatggcg gcgaaccgct aagcctcttt gaatctggtg ccattttgtt gtatctggct
     9421 gagaaaacgg ggctcttttt gagtcatgaa acgcgtgaac gcgccgccac attacagtgg
     9481 ttattctggc aggtaggcga actggggccg atgcttgggc aaaatcatca ttttaatcac
     9541 gcagcccccc aaaccattcc ttacgctatt gaacgttatc aggttgaaac tcagcgtctt
     9601 taccatgtac taaacaagcg gctggaaaac tcgccctggc tgggaggcga gaactacagc
     9661 attgcggata ttgcctgctg gccgtgggtt aatgcctgga ctcgccagcg aattgacctc
     9721 acaatgtatc cggcagtcaa gaactggcat gagcggatcc gttcgcgccc tgccaccggg
     9781 caggcactgc taaaagcaca actcggtgat gagcgttcgg atagttaaca gaaacaggtt
     9841 ctcgtgtatt atttcatcct aagtaaaaca acggagaacc tgcaatggca caacctgccg
     9901 ctattattcg tataaagaac cttcgtttac gtacgtttat cggaattaag gaagaagaaa
     9961 ttaacaaccg tcaggatatt gttatcaatg tgacgatcca ctaccccgcc gataaagcgc
    10021 gcactagcga agatatcaac gatgcgctga attatcgcac cgtaacgaaa aacattattc
    10081 agcatgtaga gaataaccgt ttctctttgt tggaaaaatt aactcaggat gtgctcgata
    10141 tcgcacgtga acatcactgg gtgacgtatg ctgaagtgga gatcgataaa ctgcacgcgc
    10201 tgcgctacgc cgattcggta tccatgacct taagctggca gcgttaaccg ccatatcggg
    10261 aggctgcatg aacatagtga tcaccggagg gacgggatta attggtcgcc atttgattcc
    10321 gcgtttgctg gagctgggcc atcaaatcac ggtagtgacg cgtaacccac agaaagccag
    10381 ttccgttctc ggccctcggg tgacgctatg gcaagggcta gccgatcaaa gcaacctcaa
    10441 cggcgttgat gcggtaatca acctggccgg agaaccgatt gctgataaac gctggactca
    10501 cgagcaaaaa gagcgtctct gccaaagccg ctggaatatc acgcaaaaac tggtcgattt
    10561 gattaatgcc agcgacacgc cgccgtcggt actcatttcc ggctcggcaa cgggctatta
    10621 tggcgactta ggtgaagtgg tggttaccga agaggaaccg ccgcataacg aatttactca
    10681 taaactctgc gcccgctggg aagagattgc ctgccgggcg caaagtgaca aaacgcgagt
    10741 gtgcctgctg cgaaccggcg tggtactggc accggacggc ggtattctcg gtaaaatgct
    10801 gccaccgttt cgtcttggcc tgggcgggcc gattgactct ggtcggcagt atctggcctg
    10861 gattcatatt gatgatatgg tcaacggtat tctctggctg ctggataacg agctgcgcgg
    10921 gccatttaat atggtttcgc cctacccggt acgcaatgaa caatttgccc atgcgctcgg
    10981 tcacgcgctg catcgcccgg ccattttgcg cgtccccgcg accgccattc gactgttaat
    11041 gggcgaatct tcagtactgg tattaggtgg acaacgcgcg ctgcctaaac ggctggaaga
    11101 agcgggtttt gcgtttcgct ggtacgattt agaagaggcg ctggcggatg tcgttcgctg
    11161 atgtggttta cagcaaacat ccgccagtta actcccggtg ttacaggatt agtggctttg
    11221 cgcgataaga tcgtctggtg aaagtcgggt caccatcata actaactctc tgtctaaacc
    11281 tctatccagc atctcctgag caatacgcag ggcttcttcg tgtttgccct gcattgcgcc
    11341 ttcttcacgt aatctgtcag caatggtcat cagtttctcc ttttcttgtg gtgcgcgttc
    11401 cgctatctca ccaataaatg cacgaaaacg ctgggcatcc cctgtttgta atacgtaatt
    11461 aaacagggct tttagctgtc tgtcattagt gttccctgta actagcagcg aaacaatttg
    11521 gtcgactaat cccaacagat cgcgctgacg aatatgtttc tgaattaact ccaacagcgc
    11581 cattttgcgg tgttgcataa tctcgtcatc cggcaccacg gtaatatcca ccaacggaaa
    11641 cgctgatgaa tatattttgc gggctatagc aggctcggca aattcatcaa gccagcagag
    11701 tgaataagga taaggacttc tgcaaccatg ataaaacagc atcgggatca ccaatggaag
    11761 ctctttatag cccgc
//
LOCUS       DAJVUJ010000131        11745 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.131, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000131 DAJVUJ010000000
VERSION     DAJVUJ010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11745
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.131"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(39..353)
                     /locus_tag="OQQ48_003175"
                     /pseudo
     CDS             complement(39..353)
                     /locus_tag="OQQ48_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
     gene            complement(468..866)
                     /locus_tag="OQQ48_003176"
     CDS             complement(468..866)
                     /locus_tag="OQQ48_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HCS1953857.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQSNQSVSGEQLESAPGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSSQANNDLLSAGMELLKGKLFR"
     gene            complement(1106..3520)
                     /gene="gyrB"
                     /locus_tag="OQQ48_003177"
     CDS             complement(1106..3520)
                     /gene="gyrB"
                     /locus_tag="OQQ48_003177"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCS1953858.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADLLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(3549..4622)
                     /gene="recF"
                     /locus_tag="OQQ48_003178"
     CDS             complement(3549..4622)
                     /gene="recF"
                     /locus_tag="OQQ48_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCS1953859.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWACFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLVEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(4622..5722)
                     /gene="dnaN"
                     /locus_tag="OQQ48_003179"
     CDS             complement(4622..5722)
                     /gene="dnaN"
                     /locus_tag="OQQ48_003179"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCS1953860.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(5727..7130)
                     /gene="dnaA"
                     /locus_tag="OQQ48_003180"
     CDS             complement(5727..7130)
                     /gene="dnaA"
                     /locus_tag="OQQ48_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCS1953861.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            7427..7507
                     /locus_tag="OQQ48_003181"
     CDS             7427..7507
                     /locus_tag="OQQ48_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1953862.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            7737..7877
                     /gene="rpmH"
                     /locus_tag="OQQ48_003182"
     CDS             7737..7877
                     /gene="rpmH"
                     /locus_tag="OQQ48_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCS1953863.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            7894..8253
                     /gene="rnpA"
                     /locus_tag="OQQ48_003183"
     CDS             7894..8253
                     /gene="rnpA"
                     /locus_tag="OQQ48_003183"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCS1953864.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            8217..8474
                     /gene="yidD"
                     /locus_tag="OQQ48_003184"
     CDS             8217..8474
                     /gene="yidD"
                     /locus_tag="OQQ48_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCS1953865.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            8477..10123
                     /gene="yidC"
                     /locus_tag="OQQ48_003185"
     CDS             8477..10123
                     /gene="yidC"
                     /locus_tag="OQQ48_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCS1953866.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKTQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            10229..11593
                     /gene="mnmE"
                     /locus_tag="OQQ48_003186"
     CDS             10229..11593
                     /gene="mnmE"
                     /locus_tag="OQQ48_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCS1953867.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT         2671 a         3233 c         3048 g         2793 t
ORIGIN      
        1 ggacaggggt atcgcttagt ttttcaattt cctatacgtg aaccgacttc gcgagagagt
       61 ttttccagga aacgatagtc gtcataactg agagcagttt gcgccacggt gctaccatca
      121 gcggccttct gtaccagcgc gccgttataa gtaatgcagt agtcgcccgg ctgttccata
      181 tgcagctctt tcaggtagtt gtgaacgcct gcataagggc gacccgtcgt tagcacgaca
      241 ttcacgccac gggcgcgagc tgcggcaatc gcatttttaa cggcgggtga aatggtgtga
      301 tcgggcagca gaagggtgcc atccatatcg atagcaatga gtttgatagc catgagttcc
      361 ccaggtagat tagttcctga cccatgctaa cgcgattccg ctcaaaaatc agtacaacac
      421 ccgagggaaa agggggatgc aacgcgggtt gcatgctccc catttgctta gcggaagagt
      481 ttccctttca gcagttccat acctgcggaa agcagatcgt tattggcttg tgaagacact
      541 tcaccttgcg gtgagagcgc atcaataatc ttcggcaatt gttctgccag taaactggaa
      601 gccgtactgg tatccacgcc aagtttttgc ccgagatcgg acaccgcatt tgtgccgggc
      661 gccgattcca gttgctcgcc gctaactgat tgattgctct gctgattact caaccaggtt
      721 gagagaatgg cccctaagcc gccactttgc agtttttcca gcagcacctg aatgccgccc
      781 tgctcctcaa cccaacttaa aatagcctga tatttccccg catcgccttt cagaaaggca
      841 ccgacaactt catcaaaaag ccccatgata atcacctgta aagcgttacg tgttgaccca
      901 aaaagtatag atctgcggga tgataattgc ggattgcaga aataaaaagg gcggagatga
      961 tctccgccct ttttttatag cttcttgccg gatgcggcgt taacgtctta cctggcctac
     1021 aaaatcatta aaattcaata cattgcaaga ttttcgtagg cctgataagc gtagcgcatc
     1081 aggcacgctc gcatggttaa cgccattaaa tatcaatatt cgccgctttc agggcgttct
     1141 cttcaataaa cgcacgacgc ggttctacgg cgtcgcccat cagcgtggtg aacaacaggt
     1201 cagcggcaat cgcatcctta acggtaacgc gcagcatacg acgactttcc gggtccatgg
     1261 tggtttccca cagctgttcc gggttcatct cgcccagacc tttataacgc tggatggaga
     1321 gaccgcgacg agactctttc accagccagt ccagcgcctg ctcgaagctg gctaccggct
     1381 gacgacgctc gccacgttcg ataaatgcat cttcttccag caagccacgc agtttctcac
     1441 ccagcgtgca gatacgacga tattcgccac cagtgataaa ttcgtgatcc agcggatagt
     1501 cagtatccac accgtgggta cgcacgcgaa caatcggctc gaacaggttt tgttcggcat
     1561 tggtgtggac atcaaacttc cactggctgc cgtgctgttc tttgtcgttc agttcgctga
     1621 ccagcgcgtt cacccagcgg gtaacggtct gctcatcaga gaggtcggct tccgtcagcg
     1681 tcggctgata gataagctct ttcagcattg ctttcggata acgacgctcc atgcggttga
     1741 tcattttctg cgtcgcgttg tactcagaca ccagtttctc taacgcttcg ccagccaatg
     1801 ccggtgcgct ggcgttggtg tgcagcgttg cgccgtccag cgcgatagag atctggtact
     1861 gatccatcgc ttcgtcgtct ttaatgtact gttcctgctt gcctttcttc actttgtaca
     1921 gcggcggctg agcgatgtag acgtgaccgc gttcaacgat ttccggcatc tgacgataga
     1981 agaaggtcaa cagcagcgta cgaatgtgcg agccgtcgac gtccgcatcg gtcatgatga
     2041 tgatgctgtg ataacgcagt ttgtccgggt tgtactcgtc acgaccgata ccacagccaa
     2101 gcgcggtgat aagcgtcgcc acttcctgag aagagagcat cttatcgaag cgcgctttct
     2161 caacgttgag gattttaccc ttcagcggca gaatcgcctg gttcttgcgg ttacgcccct
     2221 gcttcgcaga gccgcccgcg gagtcccctt ccaccaggta cagttcggaa agcgccggat
     2281 cgcgttcctg gcagtctgcc agtttgcccg gcaggcccgc taagtcgagc gcacctttac
     2341 ggcgggtcat ttcacgcgcg cgacgcgccg cttcacgggc acgggcagca tcgataattt
     2401 tgccaaccac gattttcgcg tcggttgggt tttccagcag gtattctgcc agcagttcgt
     2461 tcatctgctg ttcaaccgcc gatttcacct cagaagaaac cagtttgtct ttggtctggg
     2521 aggagaattt cgggtccggc acttttacgg aaacgaccgc aatcaggcct tcacgcgcat
     2581 cgtcaccggt ggcgctgact ttggcttttt tgctgtagcc ttctttgtcc atgtaggcgt
     2641 tcagggtacg ggtcatcgcc gcacggaagc ctgccaggtg agtaccgccg tcacgctgcg
     2701 gaatgttgtt ggtaaagcag tagatgtttt cctggaagcc atcgttccac tgcaacgcca
     2761 cttcgacgcc aataccgtct ttttcggtgg agaagtagaa gatattcgga tggatcggcg
     2821 ttttgttctt gttcagatat tcaacgaacg ccttgatgcc gccttcatag tggaagtggt
     2881 cttctttgcc gtcacgcttg tcgcgcagac gaatggaaac gccggagttg aggaacgaca
     2941 actcacgcag acgtttcgcc agaatttcat attcgaactc ggtcacattg gtgaaggttt
     3001 cgaggctggg ccagaaacgc accatggtgc cggttttttc agtctcgccg gtaaccgcca
     3061 gcggagcctg cggtacaccg tgttcgtaga tctgacggtg aattttaccc tcgcgctgga
     3121 taaccagctc cagtttttgc gacagggcgt ttactaccga aacaccaacg ccgtgcagac
     3181 cgccggacac tttataggag ttatcgtcaa atttaccgcc tgcgtgcaga acggtcatga
     3241 tcacttccgc cgccgatacg ccctcttccg ggtgaatacc ggtcggaatg ccgcgcccgt
     3301 catcctgtac agagacagag ttatcggcgt gaatggtgac gataatttct ttacagtgac
     3361 ccgcgagcgc ttcgtcgata gcgttatcta ccacctcgaa taccatgtgg tgcagaccgg
     3421 tgccgtcatc cgtgtcgccg atatacatac ccgggcgctt acgcaccgca tccagccctt
     3481 tcaggacttt gatactggag gagtcataag aattcgacat caacgtttct cgctcattta
     3541 tacttgggtt aatccgttat tttacccttt tccacggtaa acatcttcga attttcgtcc
     3601 gacatgtcta taacgtgttc agcactgatc gcgctgacaa agacctgtga ttgcgtcgct
     3661 tttaagcggc tggcaagcag cccgcgacgc tcatcatcaa gctcagaggc aaaatcatct
     3721 atcaggtaga gacaccgccg cccgctttca cgggtgagga actctccttg cgccagacgt
     3781 aaggcgcaca tcaacagctt aagctgccca cgcgataagg tatcttccac cggcgcaccg
     3841 tcggcgcgaa tgcgtaagtc cgctttatgc gggccatgcg cggtgtaggt tagctggcga
     3901 tcgcgttcaa aattacgttc cagcacctca gcatattctg tctctttctc ccagccgcgc
     3961 tggaaagaga aagtcagaga aaactcaggg agaaattgct tacaagtatc ggccatatca
     4021 gccgcgatac cggcgctata ctccgcgcgc caggtgctga tttgctccac cagcgggatc
     4081 agctctttat cccacgggcg tagctgttcg taacgtgtca cctggcgcag cgccgcattg
     4141 cgctgcttga gcaatcgctt gagattgctc caggcggtga aaaatccggg ttcgttgtga
     4201 aagcatgccc agtcgagaaa tgctcttctg tatttggggc cgccgttgag taaagtaaac
     4261 ccttctggcg ttatcagctg catcggcatc aggtgcgcca gctccgcgac tttatgcccg
     4321 tcagtaccgt cgatgcggac tttgctgtcg ccttgtttgt ctttggttaa gccaatcgcc
     4381 gtctcgcgct cttcgccctg taatcgccca tggagaacaa atgcctcctg ctcatggcga
     4441 atgacgcgac caatctgcaa actgcgaaac gcccgaccat ggccgagcgt atagatggct
     4501 tccagcacac tggttttgcc actgccgttg gcacctacca gaaagttaaa gccgggagat
     4561 aaggcgagat ccgcggtttc gatattgcgg aaatcgcgga tcaacaagcg ggtgagggac
     4621 attacagtct cattggcatg acaacataag ccgcgctctg gctggccgca tcttcaatct
     4681 gcacgctgga aaccgaatcg gtcagcatca tgcggacgtt ttcgcatttc agcgcgttca
     4741 gaacatccag cacatagctg acgttgaagc cgatttccat ctccgcaccg ctataggtaa
     4801 cgtcgaggat ctcttccgct tcttcctgtt ccgggttgtt ggcagtgatt ttcagctggt
     4861 tttcgctgac ataaagacgt acgccgcgga atttctcgtt agagagaatt gccgcacggg
     4921 caaacgcctg cttgagcaga tcgcagccag cttccagatg tttgtccggg ttcttcggta
     4981 gaacgcggcg gtaatccggg aagcgaccat ccaccagttt ggaggtgaag ataaagtcgc
     5041 caacgtgggc acgaatgtta ttgctgccaa tctgcacgcg cagcggattg tcgccgccgt
     5101 cgagcatacg catcagttca atcacgcctt tacgcggcac gatcaccgaa tggcttggca
     5161 aagattgacc aattggcatt gaacagactg ccagacggtg accatcggtc gctacggtgc
     5221 gcagttcttc gccttcggtt tcaaacagca taccgtttaa gtaatagcga acatcctgat
     5281 gcgccatcga aaactgggtc gcttcaatca gacgcttcat cgtcgcctgc ggcagggtaa
     5341 attcgacttc gctctgccag tcatcgaggt tcgggaaatc cgccgctggc agggtagaca
     5401 gcgaaaaacg gctacgcccg gagcgcacca gcatccgttc accttccagc tgcacggcaa
     5461 tttccgcgcc ttcaggcaga ccacggcaga tatcaaagaa tttgcgcgcc ggaacggtcg
     5521 ttgctcctgg ctcgtgtggc tgaaccagcg caacacgtgc caccatttcc atctcgagat
     5581 cagtaccggt cagcgacaac gtaccgtcag caacctgtaa cagcagatta ccgagaatcg
     5641 gtagcgtagg acgaccacct aacggaccgc tcacctgttg tagcggtttt aataaatgct
     5701 cacgttctac ggtaaatttc ataggtttac gatgacaatg ttctgattaa atttgaaaaa
     5761 tcttctttga tatcgtggct ctcttcacgc aactgctcga tcttacggca ggcatgaagc
     5821 accgtcgtgt ggtcacgacc accaaacgca tcgccaatct ccggcagact gtggttagtc
     5881 agctctttcg ccagcgccat cgccatctgg cgcggacgcg ccaccgagcg ggatcgacgc
     5941 ttggaaagga gatccgcgac tttgatcttg tagtactccg ccaccgtctt ctgaatattg
     6001 tcgatggtga ccagtttttc ctgcaatgcc agcaagtcgc gcagcgcctc acgcacgaag
     6061 tcgatggtga tcgcccgtcc ggtaaagttg gcattggcaa tgacgcggtt cagcgcccct
     6121 tccagctcac gtacgttaga tcgtagacgc ttggcgataa agaacgccac ttcgcccggc
     6181 aaacgaatgt cgttttcgtc ggcctttttc atcaggatcg ccacacgggt ttccagctct
     6241 ggcggttcga tcgccacagt cagtccccaa ccgaagcggg atttcaaacg atcctcaacg
     6301 ccgttgatct ctttcggata gcgatccgag gtgagaatga tctgttgatt accttccagc
     6361 agggcgttga aggtatggaa aaactcttcc tgagatcgtt ctttattagc aaaaaactga
     6421 atatcgtcga tcagcagtgc atctacggaa cggtagtagc gtttaaactc ttcgatcgcg
     6481 ttgttttgca gggctttaac catgtcctga acaaagcgct cggagtgcat ataaaccact
     6541 ttggcattcg gcttgcgcgc cataatgccg ttacccaccg catgcagcag gtgagtttta
     6601 cccagacccg tgccgccata aaggaacaac gggttatagg caccgccagg gttatccgcc
     6661 acctggcgag ccgccgcgcg cgccagttgg ttagatttac cttcaacgaa gttatcaaac
     6721 gtgtgtttga cgtttacgtt agaacgatag gtcggttctg ccggggccgg gacgttatcc
     6781 caacctgagc gcgtagaagg cgcagcacgt tgcggctgcg tttgcgccac ctgtgcaggg
     6841 gccgcgacgt tgctcgtcac tgccgcttgt ggcgtttgcg tcaccggttt ggtgccgact
     6901 tcaaaacgca gctgtggggc atccgctccg cagaaactgg ttagcagtcc attgatatta
     6961 ttaaggtact tgtcccgtac ccaatcgagg acaaaacggt ttggcgcgta cagggccagc
     7021 gtgttatcgc tcagttccgc ctgcaatggg cgtatccaca tactgaattc tgtggctggt
     7081 aactcatcct gcaatcgggc aagacactgc tgccaaagcg aaagtgacac ggcggactcc
     7141 actcgaacaa aagtcgataa tgactaaggc tgaaacattc atgattgttg acgtacgtcg
     7201 aaaagaccct gcttgagggt gacgcacgaa ccgctgtctg cggttatatg cccgatcaag
     7261 atcctgcaaa acgatcggga ccgcggatca tagcctaaac tgcgcaagag atcttctgtt
     7321 tctcacagat tttcccgatt tatccacagg actttccaga actcgctaag tgtaaacgat
     7381 cctgccgcga ggcgggcacg atttacgccg catattgaaa aatttaatga ccatagacaa
     7441 aaattggctt aatcgatcta ataaagatcc aggacgatcc ttgcgcttta cccatcagcc
     7501 cgtataatcc tccacccggc gcgccatgct ggtttccact ggtgtgaggt cgtacatttt
     7561 ccctgcgaaa aggtgcggaa aagcgcggta aataaggaaa gagaattgac tccggagtgt
     7621 acaattatta caatccggcc tctttaatca cccatggctt cggtgtccat cgtttcattt
     7681 ttcggcggat atccaataaa gccattgaat ttattcaagt ttaggtagaa atcgccatga
     7741 aacgcacttt tcaaccgtct gtactgaagc gcaaccgttc tcacggcttc cgtgctcgta
     7801 tggctactaa aaatggtcgt caggttctgg cacgtcgtcg tgctaaaggc cgcgctcgtc
     7861 tgaccgtttc taagtaataa agctaacccc tgagtggtta agctcgcatt tcccagggag
     7921 ttacgcttgt taactcccag tcaattcaca ttcgtcttcc agcagccaca acgggctggc
     7981 acgccgcaaa ttaccattct cggccgcctg aattcgctgg ggcatccccg tatcggtctt
     8041 acagtcgcca agaaaaacgt tcgacgcgcc catgaacgca atcggattaa acgtctgacg
     8101 cgtgaaagct tccgtctgcg ccaacatgaa ctcccggcta tggatttcgt ggtggtggcg
     8161 aaaaaagggg ttgccgacct cgataaccgt gctctctcgg aagcgttgga aaaattatgg
     8221 cgccgccact gtcgcctggc tcgcgggtcc tgatagccct cattcgggtc tatcaacgcc
     8281 tgattagtcc gctactcggg ccgcattgtc gtttcactcc aacctgttca agctacggaa
     8341 ttgaggcatt gcgcaggttt ggagtgataa aaggcagttg gttgacggtg aaacgcgtat
     8401 taaaatgcca ccctttacac cctggtggtg acgatcccgt cccgcccgga ccatttgata
     8461 ccagagaaca ctaacgatgg attcgcaacg caatctttta gtcatcgctt tgctgttcgt
     8521 gtctttcatg atctggcaag cctgggagca ggataaaaac ccgcaacctc aggcccaaca
     8581 gaccacgcag acaacgacca ccgcagcggg tagcgccgcc gaccagggcg taccggccag
     8641 tggccagggg aaactgatct cggttaagac cgacgtgctt gatctgacca tcaacacccg
     8701 tggtggtgat gttgagcaag ctctgctgcc tgcttacccg aaagagctga actctaccca
     8761 gccgttccag ctgctggaaa cttcaccgca gtttatttat caggcacaga gcggtctgac
     8821 cggtcgtgat ggcccggata acccggctaa cggcccgcgt ccgctgtata acgttgaaaa
     8881 agacgcttat gtgctggctg aaggtcaaaa cgaactgcag gtgccgatga cgtataccga
     8941 cgcggcaggc aacacgttta ccaaaacgtt tgtcctgaaa cgtggtgatt acgctgtcaa
     9001 cgtcaactac aacgtgcaga acgctggcga gaaaccgctg gaaatctcca cctttggtca
     9061 gttgaagcaa tccatcactc tgccaccgca tctcgatacc ggaagcagca acttcgcact
     9121 gcacaccttc cgcggcgcgg cgtactccac gcctgacgag aagtacgaga aatacaagtt
     9181 cgataccatt gccgataacg aaaacctgaa catctcttcg aaaggtggtt gggtggcaat
     9241 gctgcaacag tatttcgcga cggcgtggat cccgcataac gacggtacca acaacttcta
     9301 taccgctaat ctgggtaacg gcatcgccgc tatcggctat aaatctcagc cggtactggt
     9361 tcagcctggt cagactggcg cgatgaacag caccctgtgg gttggcccgg aaatccagga
     9421 caaaatggca gctgttgctc cgcacctgga tctgaccgtt gattacggtt ggttgtggtt
     9481 catctctcag ccgctgttca aactgctgaa atggatccat agctttgtgg gtaactgggg
     9541 cttctccatt atcatcatca cctttatcgt tcgtggcatc atgtacccgc tgaccaaaac
     9601 gcagtacacc tccatggcga agatgcgtat gctgcagccg aagattcagg caatgcgtga
     9661 gcgtctgggc gatgacaaac agcgtatcag ccaggaaatg atggcgctgt acaaagctga
     9721 gaaggttaac ccgctgggcg gctgcttccc gctgctgatc cagatgccaa tcttcctggc
     9781 gttgtactac atgctgatgg gttccgttga actgcgtcag gcaccgtttg cactgtggat
     9841 ccacgacctg tcggcacagg acccgtacta catcctgccg atcctgatgg gcgtaacgat
     9901 gttcttcatt cagaagatgt cgccgaccac agtgaccgac ccgatgcagc agaagatcat
     9961 gacctttatg ccggtcatct tcaccgtgtt cttcctgtgg ttcccgtcag gtctggtgct
    10021 gtactatatc gtcagcaacc tggtaaccat tattcagcag cagctgattt accgtggtct
    10081 ggaaaaacgt ggcctgcata gccgcgagaa gaaaaaatcc tgattcggtg agttttcgct
    10141 aaaataaggg cggtcagttg accgcctttt ttcttttcgt agggcggata agcaccgcgc
    10201 atccgccaca caaagcaaca ggaacatcat gagcgataat gacactatcg tagcccaggc
    10261 cacgcctccg ggacgtggcg gcgttggcat cctgcgcatc tccggcttca aagcccgtga
    10321 agttgccgaa accgtgctgg gtaaactgcc taagccgcgc tacgccgatt atcttccgtt
    10381 taaagacgcc gacggcagcg tgctcgatca ggggattgcg ctatggttcc ctggcccgaa
    10441 ctcgttcacc ggcgaagatg tgctggaact gcaaggtcat ggcggtccgg tgatcctcga
    10501 cctgctgtta aaacgcattc tgaccattcc cggcctgcgg attgctcgcc ctggtgagtt
    10561 ttccgaacgc gcgtttctta acgataaact tgacttagcc caggccgagg cgattgccga
    10621 tcttatcgac gccagttcgg aacaggcggc ccgttcggca cttaactcgc tgcaaggcgc
    10681 attctccgca cgggttaatc atctggtaga agccctcacc cacttgcgca tttacgtcga
    10741 agcggcaatt gatttccccg atgaagagat cgatttcctc tccgacggaa aaattgaagc
    10801 ccagctcaat gacgttattg ccgatcttga tgcagtgcgt gctgaagcac gtcagggtag
    10861 tttgttgcgc gaagggatga aagtggtgat tgccggacgt cctaacgccg gtaaatcgag
    10921 cctgttaaac gcgctggcgg ggcgtgaagc ggcaatcgta accgatatcg ccggaactac
    10981 gcgtgacgtg ctgcgtgagc atatccacat tgacggaatg ccgctgcata tcatcgatac
    11041 cgccgggcta cgtgaagcca gtgacgaagt agaacgtatt ggtatcgagc gcgcgtggca
    11101 ggaaattgaa caggccgacc gcgtgctgtt tatggtcgat ggcaccacaa cagacgccgt
    11161 ggatccggca gagatctggc cggaatttat tgcccgtctg ccagcgaaac tgccgatcac
    11221 cgtggtgcgc aataaagccg atatcaccgg cgaaacgctg ggaatgagtg aagtgaacgg
    11281 tcacgcgtta attcgtctct cggcaaggac tggtgaaggc gtggacgtgc tgcgtaacca
    11341 tctcaaacag agcatgggct ttgacaccaa catggaaggc ggcttcctgg cgcgtcgtcg
    11401 ccacctacag gcgctggaac aggcagcgga acatctacaa cagggcaaag cgcaactgtt
    11461 gggagcctgg gcaggtgaac tgctggcgga agagttgcgt ctggcacagc agaacttaag
    11521 cgaaatcacc ggggaattta cttcagacga cctgctgggg cggattttct ccagcttctg
    11581 tattggtaag taaccgcgct tacgaagccg tattctgact gtcagatgcg gcttcgcttc
    11641 attgttagca ctcctgttat tcctcaaccc tttttttaaa cattaaaatt cttacgtaat
    11701 ttataatctt aaaaaaagca tttaatattg ctccccgaac gattg
//
LOCUS       DAJVUJ010000132        11731 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.132, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000132 DAJVUJ010000000
VERSION     DAJVUJ010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11731
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.132"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            29..1414
                     /locus_tag="OQQ48_003187"
     CDS             29..1414
                     /locus_tag="OQQ48_003187"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCS1953868.1"
                     /translation="MEGGWNEGGKGISIADVMTAGAHGVPREVTEGVIDGLNYPNHEA
                     IDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEPNEEGLQFYDDLFDECLKQ
                     GMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTVFTRYKEKVKYWMTFNEIN
                     NQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYELVASALAVQTGKSINPEFN
                     IGCMIAMYPIYPLTCVPNDMMMATKAMHRRYWFTDVHARGYYPQHMLNYFARKGFNLD
                     ITPEDNAILASGCVDFIGFSYYMSFTTQFSPDNPQLDYVEPRDLVSNPYIDTSEWGWQ
                     IDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTVNDHYRIDYFASHIREMKK
                     AVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKDNEGKGTLERIRKASFYWY
                     RDLIANNGENI"
     gene            complement(1471..1689)
                     /gene="marB"
                     /locus_tag="OQQ48_003188"
     CDS             complement(1471..1689)
                     /gene="marB"
                     /locus_tag="OQQ48_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HCS1953869.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(1721..2104)
                     /gene="marA"
                     /locus_tag="OQQ48_003189"
     CDS             complement(1721..2104)
                     /gene="marA"
                     /locus_tag="OQQ48_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HCS1953870.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            complement(2124..2558)
                     /gene="marR"
                     /locus_tag="OQQ48_003190"
     CDS             complement(2124..2558)
                     /gene="marR"
                     /locus_tag="OQQ48_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HCS1953871.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            2770..3435
                     /gene="marC"
                     /locus_tag="OQQ48_003191"
     CDS             2770..3435
                     /gene="marC"
                     /locus_tag="OQQ48_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HCS1953872.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            complement(3460..4650)
                     /gene="ydeA"
                     /locus_tag="OQQ48_003192"
     CDS             complement(3460..4650)
                     /gene="ydeA"
                     /locus_tag="OQQ48_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HCS1953873.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(4800..5914)
                     /gene="yneK"
                     /locus_tag="OQQ48_003193"
                     /pseudo
     CDS             complement(4800..5914)
                     /gene="yneK"
                     /locus_tag="OQQ48_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
     gene            complement(5992..6873)
                     /locus_tag="OQQ48_003194"
     CDS             complement(5992..6873)
                     /locus_tag="OQQ48_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1953874.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAIVLRVPSNLTIRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVPDSAKQGYQ"
     gene            6974..8363
                     /gene="sad"
                     /locus_tag="OQQ48_003195"
                     /pseudo
     CDS             6974..8363
                     /gene="sad"
                     /locus_tag="OQQ48_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
     gene            8427..9353
                     /gene="glsB"
                     /locus_tag="OQQ48_003196"
     CDS             8427..9353
                     /gene="glsB"
                     /locus_tag="OQQ48_003196"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HCS1953875.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            9353..9712
                     /locus_tag="OQQ48_003197"
     CDS             9353..9712
                     /locus_tag="OQQ48_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HCS1953876.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            9851..11269
                     /locus_tag="OQQ48_003198"
     CDS             9851..11269
                     /locus_tag="OQQ48_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCS1953877.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIVAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVVMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            11496..>11731
                     /locus_tag="OQQ48_003199"
     CDS             11496..>11731
                     /locus_tag="OQQ48_003199"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="catalyzes the formation of D-tagaturonate from
                     D-altronate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HCS1953878.1"
                     /translation="MKTLNRRDIPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNE"
BASE COUNT         2973 a         2651 c         3015 g         3092 t
ORIGIN      
        1 atggggcggc gcggtagccg cgcatcagtt ggaaggtggc tggaatgaag gaggaaaagg
       61 catcagtatc gctgatgtga tgactgctgg cgctcacggg gtgccgcgtg aagtgacaga
      121 aggcgttatc gacgggctta attatcccaa tcatgaagca attgattttt atcatcgcta
      181 taaaacagat attcagttat ttgccgagat gggattcaaa tgctttcgaa cttccattgc
      241 ctggacacga atctttccgc aaggtgacga acaggagccg aatgaagagg gtttacaatt
      301 ttatgatgat ctgttcgatg aatgccttaa gcagggaatg gaacctgtgg tgacgctttc
      361 acattttgag atgccttatc atctggtgac aaaatatggt ggctggcgaa accgtaaact
      421 gatcgacttt tttatccgct tcgcatcaac ggtcttcacg cgctataaag aaaaagtaaa
      481 gtactggatg acgtttaacg aaatcaataa tcaggtgaat ttcagcgaaa gcctgtgtcc
      541 atttactaat tccggtatct tgtattcgcc agaggaagat atcaatgagc gcgaacaaat
      601 aatgtaccag gcggtacatt acgagttagt tgccagtgcc ctggcggtac agactggaaa
      661 atcgatcaat cctgaattta acattggctg tatgatcgcc atgtatccca tctatcctct
      721 gacgtgtgta cccaacgata tgatgatggc cacgaaagca atgcatcgtc gttactggtt
      781 tactgatgtt catgctcgtg gatattatcc gcaacatatg ctgaattact ttgccaggaa
      841 aggattcaac ctcgatatca caccagaaga taacgcgatt cttgccagtg gttgtgtcga
      901 ctttatcggc tttagctact acatgtcttt tacgacacaa ttttcgcccg ataacccgca
      961 actggattat gttgaaccac gagatttggt cagcaaccct tatatcgata catccgaatg
     1021 gggatggcaa attgatccgg cagggctacg ttattcactc aactggttct gggatcattt
     1081 ccagttgccg ctgtttattg tcgaaaatgg atttggtgcg gttgaccaga gacaagctga
     1141 cggcacggtg aacgatcact atcgcattga ttactttgct tcccatattc gggaaatgaa
     1201 aaaagccgta gttgaagatg gtgttgactt aattggctac accccgtggg gctgcattga
     1261 cctggtttct gccggaacag gggaaatgaa aaagcgctac ggaatgattt atgtcgataa
     1321 agacaacgaa gggaagggaa cgctggaacg gatacgtaaa gcgtcgtttt actggtatcg
     1381 ggatctcatc gccaacaatg gcgaaaatat ttgagacttg aggaagatat tacggagatg
     1441 tcaggctctg tgatcagagc ctgacaataa ctacatagcg tgttgattat aatagggcac
     1501 gccgagcgca tccgacttat cactgccagt acccatgtga tttaaatcaa acggtggttg
     1561 ttcctgcgat gggggaacaa ccacgacatt ggcacaagaa gtaacaactg gctgcgtggt
     1621 ttgttccgca acgccctgcg cggaaaagag aataagcgca gctgctattg cggatgaaag
     1681 tggtttcatg attgcctcag tgacgttgtc acgttatcaa ctagctattg taatgattta
     1741 atggatgtaa aaagcgcgat tcgccctgca tattggtcat ccggtattta tgtggcggaa
     1801 catcaaagta atttttgaag gttcgggtca gagtttgttg cgactcgaag ccatatcgtt
     1861 ctgccagata gagtatcggc tcgttacttt ccttcagctt ttgcgcgatt tccgtcatct
     1921 tacggctgcg gatgtattgg cctaatgaat gaccggtttc ttttttaaac atccgttgca
     1981 ggtgccattt agagtaacct gaacgctctg acactttctc cagtgacagt ggcgattcca
     2041 ggttgtcctc gatccagtcc aaaatgctat gaatggtaat agcgtcagta ttgcgtctgg
     2101 acatcgtcat acctcttttt tgtttacggc aggactttct taagcaaatg ctcaagtgtt
     2161 gccacttcgt ccgccgtcag gttttttgtt aattcttgat gcaggtcctg gccaactaat
     2221 tgatggcatt gttcgcatat tgccgcgccg ctggtggtaa gttttaccag tacgccgcgc
     2281 ttatcattcg ggttcggcaa cctttcaacc cagcctttac agaccaggcg atccagcata
     2341 cgggtcagtg ctccaaggtc gacagacaac acttttttca gttcaaccgg agtaatacac
     2401 gccgcgcagc ggatagagca gagcacctta aactgtgccg cggtaatatc cagcggagac
     2461 agatactcgt taagcagacg atctttcttc tgattaacca tatggattaa gcgacccaat
     2521 ggaataattt cattgaacag atcgctggta cttttcacat tagttgccct ggcaagtaat
     2581 tagttgcagg gggtaatatt gcccaggcaa gtataagtca aataaatgaa ttgaccgatg
     2641 ccacgttttg ctaaatcggt tcaagttttg gtcaatgcga tgcaaggtta gctaaagagt
     2701 gacactttat ccttgtttac cccttatact tttcgctgat aacccagata cactggataa
     2761 caaccaccaa tgttagattt gtttaaagca attggcctgg ggctggtggt gttactgccg
     2821 ttagctaacc cattaacaac tgtcgcgtta tttcttggcc tggcaggcaa catgaacagt
     2881 gccgaacgta atcgtcagtc gttgatggcc tcggtgtacg tatttgccat catgatggtg
     2941 gcgtattacg ccgggcaact ggtgatggat acatttggca tttcgattcc cggtctgcga
     3001 attgcaggcg gcttaatcgt cgcgtttatc ggttttcgga tgctctttcc gcaacagaaa
     3061 gcgattgatt caccggaggc gaaaagcaag tcggaagagc tggaagatga acccagtgcc
     3121 aatattgcct ttgtgccgct agcaatgcca agtaccgctg gtccgggaac catagcgatg
     3181 attattagct cggcgtccac agtgcgtcag agttcaacct tcgctgattg ggtactgatg
     3241 gttgcgccgc cgctgatctt tttcctggtg gcggttattt tgtggggaag cctacgcagc
     3301 tccggcgcaa ttatgcggct ggtgggcaag ggtggcattg aagcgatctc ccgtctgatg
     3361 ggattcctgc tggtatgtat gggtgtgcag tttattatta acggcatcct ggaaatcatt
     3421 aaaacgtacc attaaaaaag gcccgaatgg gcctttcaac tattgcgtct gttcttcgag
     3481 tgtcactggc cagcggcgaa atataatgat tgaccatatt aacgcggcaa aagcaggcac
     3541 cgcgcccaca taaccaatca tcgacattga ccagtgcaaa ctcacctgat tacctaccaa
     3601 cgcacccgcc ccgattccaa tattaaatat gccggagaat agcgccatcg cgacgtcggt
     3661 agcatctggt gccagcgcca gcactttaac ctgcatacca agcccgatga tcatcatcgc
     3721 gatcccccag aaaatactca gcaccccgag gtgtatttca ctgttcgccg caggtagcag
     3781 cagtgccaga cacaccagca acagcgcaat cgccgtactc accaacgcag acgcatactg
     3841 attacccagt ttaccgaaaa tcacgctgcc aataatgccc gcaccaccga gtaataacag
     3901 taatgccgtg gcaaagttgg cgctgaatcc cgcaatgttt tgcacaaaag gctcgatata
     3961 gctgtatgcc gtgtaatggg cggtgacaac caccacagtt aacaaataaa tgctcatcaa
     4021 tgccgggcgg cggaacaata gcgggaggct tttcaatgaa ccggaatgct cactgggcag
     4081 taagggaagt aacttaatca ggcacaaaag ggtgataagc gccccaatac caatcgcgaa
     4141 gaaggtcatt cgccaaccga aatactgccc cacaatgcgc ccgagaggta aacctaagac
     4201 catcgccagt gctgtaccgg tggcaattaa actcaaggcc tgtgcccgct tcccggccgg
     4261 agccatacgg atcgccagag acgccgtaat cgaccagaaa atcgcatgtg caaaagccac
     4321 accaatgcga ctgatcacca gaacggtaaa gctccacgat aaaaacgaca gtacgtggct
     4381 ggcaataaac accacaaaca ggcagatcag taatttgcgc cgttcaacct gactggtcat
     4441 taacataaaa ggcaatgaca ttagcgctac tacccatgcg taaatggtca acatgatgcc
     4501 gacctgagcg gtttgcatgt gaaaactttg cgcaatgtca gagagcaggc caacagggac
     4561 aaattcggtg gtgttgaaga tgaaggcggc gactgccagc gtaacgaccc gtagccacgc
     4621 cactttgcgg gaaacagtgt ttgttgtcat aacagattat cgggtgagtt gcgaaaaagt
     4681 gatgaaatga tcttaaaacg gttaatgatg tgaatacaat gcatttgtga tctggatcgc
     4741 gttttccgtt tctctgacgc tgattctgtt ccgcgtcaga gagtcggtaa catcacggtt
     4801 taatgacgtt cagggaggtc tggcgttttg gcggttgcgg gtataggtgg tctgacaggg
     4861 gagcgaggcc tgtttttcac caggtcttcg tgagtaactt ttggcggcgt ttcatttttt
     4921 acaaacagtt tgagggtcct gcgcgccata tgcttgataa ttttatcatc acttaatgat
     4981 gctggcatca acacgtcatt cagatcactt tcattgggat tcataaattg gtgatagtgc
     5041 aataacatat tggcgaaaaa taaaaaaaag tcctgaaaat gaggagattc ttgttctgct
     5101 aaaagggata aaaagcagat gcagacttcg ggtagtacat gtttgttaga acctaatgaa
     5161 tccatcaggc tttgcaataa atcctctttt aattcgccgt ctgttatgtc gagaatggcg
     5221 gtgatcaagt taggatcttt attgacgaac catctgtgaa cctcagagtt actccataat
     5281 cgggggagta tgacaaaatt accgcgagag ataaaattca ggacaagcaa atcaaactgg
     5341 cgtaagtaat ctgcagcggt atggtgatcc ggaggcaggg gttcaatatg tggtgtatgc
     5401 gcttttttga tggggacgcc aaggtggatc attggaaatt gttcaataaa atcattcgga
     5461 aacagaaaga gcacacgctc gacgccggtg tgaaatacgc gccccatagt gacaggcttt
     5521 gggttgggag cgcaaaaata ttaatttctc catcatggcg aaaattatct aaataaatag
     5581 ggtttaaatt tattactacc tgaaattcaa tattttcttt gtgtcttcca ttcttgacgg
     5641 gataagtgag tttaaactga accttttcat tgtatagtgt gtcgttaccc cgaaaagaag
     5701 tcctctgaat atatgataag ttatcaaggt tgttaaatat cttttttgtg gctgagttgg
     5761 tgtttaaaga cgtgcgccac tcttcatcac atattatggt actgagtgca tcaataaaat
     5821 tctttaaaac ttcttttacc tgaggtgcaa tattacgtac gggaaaatca tcaggaagaa
     5881 atatgtaatt actgatgctt actggcgtaa ccatatttgc cccatatagt cataagtcat
     5941 cggaacatca ctatataggg catttgagct gcaagaacta tcaaaaatag ctcattggta
     6001 accctgtttt gccgagtcag gcacatccag tagttgaata aatgcctcaa gcggacgcgt
     6061 tttcgcacca cgacgccaga ccagccaggt tgttaaccaa cgccattgct cagctaacgg
     6121 ccacgcttca acctggtgat gccccggcat actttccagc atagagcgtg gaataagcgc
     6181 aatgcctgct cctgcgacca cacaggccaa catcccgtga tacgactcca tctcgtgaat
     6241 agttcccgga gcggcaccgt cagcatgaaa ccagctctcg aagtggcgac gatacgaaca
     6301 attggcgcgg aaggcataaa tgttactgcc gttaacctga ctggcgcgga ttactggcgc
     6361 atgaccttgt ggcgtgacga tcatcagctc ctcgcggtat accggtatcc cgtcaatggc
     6421 agtatggtta atgggcccat caataaatgc cgcattcagt tttccctcca gcactccttc
     6481 cagcatagtg ccggaagggc cggtggaaag tgaaaactga attttgggat aacgatggtt
     6541 gaattccgcc agcgtggccg gaatgcgcac tgctgcggtg ctttccagcg aaccaagaga
     6601 aaataaacct tgcggctcat cgccagcgac aacgctccgc gcttcatcca ctaacgcgag
     6661 aatttgttgg ctatagcgta aaaagttatg cccggcagga gagagacgta aacgctgatt
     6721 ctcgcgaata aacagatcta cccccagttc tgtttccagc tggcgtagac ggatagtgag
     6781 attcgacggc acgcgaagca ctattgctgc tgcctgggtt atgctgccag cttcggcaac
     6841 cgcgttgaac atctccagtt gggtcagatc cataatattc tcgattcgtg aataagtggc
     6901 ttaatattat tcattttaaa gcaagagtaa atctgcgtat cttcatacca tgactcataa
     6961 aggagatacc ccgatgacca ttactccggc aactcatgca atttcgataa atcctgccac
     7021 gggtgaacaa ctttctgtgc tgccgtgggc tggcgctaac gatatcgaaa acgcacttca
     7081 gctggcggca gcaggctttc gcgactggcg cgagacaaat atagattatc gtgctgaaaa
     7141 actgcgtggt atcggtaagg ctctgcgcgc ccgtagcgaa gaaatggcgc aaatgatcac
     7201 ccgtgaaatg ggcaaaccaa tcaatcaggc gcgcgctgaa gtggcgaaat cggcgaattt
     7261 gtgtgactgg tatgcagaac atggtccggc aatgctgaag gcggaaccta cgctggtgga
     7321 aaatcagcag gcagttattg agtatcgacc gttggggatg attctggcga ttatgccgtg
     7381 gaactttccg ttatggcagg tgatgcgtgg cgcggttccc atcattcttg caggtaacgg
     7441 ctacttactt aaacatgcgc cgaatgtgat gggctgtgct cagctcattg cccaggtgtt
     7501 taaagatgcg ggaatcccgc aaggcgtata tggctggctg aatgccgaca acgacggtgt
     7561 cagtcaaatg attaaagatt cgcgcattgc tgctgtcacg gtgaccggaa gtgttcgtgc
     7621 gggagcggct attggcgcac aggctggagc ggcactgaaa aaatgcgtac tggaactggg
     7681 cggttcggat ccatttattg tgcttaacga tgccgatctg gaactggcgg ttaaagcggc
     7741 ggtagccgga cgttatcaga ataccggaca ggtttgtgca gcggcaaaac gctttattat
     7801 cgaagaggga attgcttctg catttaccga acgttttgtg gcagctgcgg cagccattga
     7861 aaatgggcga tccccgtgat gaagagaacg ctctcggacc aatggctcgt tttgatttac
     7921 gtgatgagct gcatcatcag gtggagaaaa ccctggcgca gggtgcgcgt ttgttactgg
     7981 gcggggaaaa gatggctggg gcaggtaact actatccgcc aacggttctg gcgaatgtta
     8041 ccccagaaat gaccgcgttt cgggaagaaa tgtttggccc tgttgcggca atcaccgttg
     8101 cgaaagatgc agaacatgcg ctggaactgg ctaatgatag tgagttcggc ctttcagcga
     8161 ccatttttac caccgacgaa acacaggcca gacagatggc ggcacgtctg gaatgcggtg
     8221 gggtgtttat caatggttat tgtgccagcg acgcgcgagt ggcctttggt ggcgtgaaaa
     8281 agagtggctt tggtcgtgag ctttcccatt tcggcttaca cgaattctgt aatatccaga
     8341 cggtgtggaa agaccggatc tgacgcgaca tattcagctc tgatatactc gcaggtcttt
     8401 tcagacctgc ggtccaggag tagaaagtgg cagtcgccat ggataatgca attttagaaa
     8461 acatcttgcg gcaagtgcgg ccgctcattg gtcagggtaa agtcgcggat tatattccgg
     8521 cgctggctac agtagacggt tcccgattgg gcattgctat ctgtaccgtt gacggacagc
     8581 tttttcaggc cggagacgcg caagaacgtt tttccattca gtctatttcc aaagtgctga
     8641 gtctcgttgt cgccatgcgt cattactccg aagaggaaat ctggcaacgc gtcggcaaag
     8701 atccgtctgg atcaccgttc aattctttag tgcaactgga aatggagcag ggtattccgc
     8761 gtaatccgtt tattaatgct ggtgcgctgg tggtctgcga tatgttgcaa gggcgattaa
     8821 gcgcaccacg gcaacgtatg ctggaagtcg tgcgcggctt aagcggtgtg tctgatattt
     8881 catacgatac ggtggtagcg cgttccgaat ttgaacattc cgcgcgaaat gcggctatcg
     8941 cctggctgat gaagtcgttt ggcaatttcc atcatgacgt gacaaccgtt ctgcaaaact
     9001 actttcatta ctgcgctctg aaaatgagct gtgtagagct ggcccggacg tttgtctttc
     9061 tggctaatca ggggaaagct attcatattg atgaacctgt ggtgacgcca atgcaggcgc
     9121 ggcaaattaa cgcgctgatg gcgaccagtg gtatgtacca gaacgcgggg gagtttgcct
     9181 ggcgggtggg gctaccggcg aaatctggcg ttggtggcgg tattgtggcg attgttccgc
     9241 atgaaatggc catcgctgtc tggagtccgg aactggatga tgcaggtaac tcgcttgcgg
     9301 gtatcgccgt ccttgaacaa ttgacgaaac agttagggcg ttcggtttat taatgcagtc
     9361 tctcgatcca ctcttcgcgc gtttatcccg ttcaaaattt cgctctcgct ttcgtctggg
     9421 catgaaagag cgtcagtatt gcctggagaa aggcgcgcca gtcatcgaac aacatgcggc
     9481 ggattttgtc gctaaacgtc ttgctccggc attaccggct aatgacggta agcaaacacc
     9541 catgcgcggg catccggtgt ttatcgctca gcacgccacc gcgacctgct gtcgcggttg
     9601 tctggctaag tggcataaca ttccgcaggg tgtttcgtta agcgaagaac aacaacgtta
     9661 tatcgtggcg gtaatctatc actggctggt tgtacagatg aatcagccat gagaattgcg
     9721 gaaaacgata aaatagcatt cccagggtac ctttatttgc cgccgctgtt agcgaacatg
     9781 ctatccttta tgagaattta cttattacgt tgagataatt gccacccgca attgtatgga
     9841 cagataatta atgcatgtac accccatttc aactttccgt ttgttccagg aaggtcatct
     9901 gctacgtaat agcatcgcta tttttgcgct aaccacgctg ttctatttta ttgttgcaga
     9961 gttacggctg gttcacgaac tttctctttt ctggccgctg aatggcgtaa tggcgggggt
    10021 gtttgcccgc tatgtctggc ttaatcgact gcattactat gcgatcagtt atgtggtgat
    10081 gctggtttat gatgccataa ccaccgaatg gggactggtt tcactggtta tcaatttctc
    10141 caatatgatg tttattgtta ccgtcgcctt actggtcgtg cgggataagc gtctggggaa
    10201 aaataagtat gagccagtaa gtgccttacg gctatttaat tactgtctga ttgccgcatt
    10261 attatgcgct attgtcgggg cgattggttc ggtcagtatt gatagtctgg atttctggcc
    10321 tttgcttgcg gactggttca gtgagcaatt ctcaacgggc gtgttgatcg tgccttgtat
    10381 gctgacgttg gctattcctg gagtgctgcc gcgctttaaa gcagaacaga tgatgcccgc
    10441 catcgcgctt attgtgtcgg ttattgcctc ggtagtcatt ggcggagcgg ggagtctggc
    10501 gttcccgctg cctgcattaa tctggtgtgc agtgcgctat acgccgcagg taacatgtct
    10561 gttgaccttt gtcaccggtg cggtggaaat cgtactggtg gcaaattcgg tgattgatat
    10621 ctcggtcggt tcgccgttct ccattccgga aatgttctcc gcacgtctcg gtattgccac
    10681 gatggcgata tgcccaatta tggtttcttt tagcgtggca gcgatcaatt cgctaatgaa
    10741 gcaagttgcg ctgcgagccg actttgattt tctgactcag gtttactcac ggtccggtct
    10801 ttatgaggcg ctgaaaagtc catcgctgaa acagacacaa catctgacgg tcatgctgct
    10861 tgatatcgac tatttcaaaa gcattaacga taactatgga catgaatgtg gcgataaagt
    10921 gttaagcgtg tttgcccggc atattcagaa gattgtcggt gataaggggc tggtggcgcg
    10981 catgggcggc gaagaatttg ctgttgcagt gccgtcggtg aatcctgtcg atggtctgct
    11041 aatggcggaa aaaatccgta aaggcgttga actgcaacca ttcacctggc aacaaaaaac
    11101 gctttatctc acggtaagta ttggcgtcgg tagtggtcgc gcatcgtacc gaacgctgac
    11161 cgatgatttt aataaattga tggtcgaagc cgatacgtgt ctgtatcgct cgaagaaaga
    11221 tgggcgcaac cgtaccagca ctatgcgtta cggtgaggaa gttgtctgac atctttattc
    11281 attccgcgcg atttgaatgc aatgtgttct accactttat gatccaggaa aaggcgtatt
    11341 tcctcgatta aaccatgatc cgcgccacac ttttctcata aaactgctca ttaatcatca
    11401 ggagttgatc aaaacagggg ttcggcgtat taaattttgc taacctgtcg taccaattca
    11461 agcgggttgt atgactaatc agaagggaac ccattgtgaa aacactaaat cgtcgcgata
    11521 ttcccggtgc acagtatcca gaacgtatca ttcagtttgg tgaaggtaac ttcctgcgcg
    11581 cctttgttga ctggcaaatc gatctcctga atgagcacac cgatctgaat tctggcgtgg
    11641 tcgttgttcg tccgattgaa acttcattcc cgccgtcact gagcacgcag gatggtctgt
    11701 acaccaccat tatccgtggc ctgaatgaga a
//
LOCUS       DAJVUJ010000133        11629 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.133, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000133 DAJVUJ010000000
VERSION     DAJVUJ010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11629
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.133"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(50..1036)
                     /locus_tag="OQQ48_003200"
                     /pseudo
     CDS             complement(50..1036)
                     /locus_tag="OQQ48_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310582.1"
                     /note="PtrB; oligopeptidase that cleaves peptide bonds
                     following arginine and lysine residues; incomplete;
                     partial in the middle of a contig; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
     gene            complement(1245..1904)
                     /locus_tag="OQQ48_003201"
     CDS             complement(1245..1904)
                     /locus_tag="OQQ48_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HCS1953879.1"
                     /translation="MANWLNQLQSLLGQSSSSISSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVRDGIERDLEQQKRTLA
                     E"
     gene            complement(2231..2587)
                     /locus_tag="OQQ48_003202"
     CDS             complement(2231..2587)
                     /locus_tag="OQQ48_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HCS1953880.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(2654..2948)
                     /gene="yebG"
                     /locus_tag="OQQ48_003203"
                     /pseudo
     CDS             complement(2654..2948)
                     /gene="yebG"
                     /locus_tag="OQQ48_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
     gene            3082..4260
                     /gene="purT"
                     /locus_tag="OQQ48_003204"
     CDS             3082..4260
                     /gene="purT"
                     /locus_tag="OQQ48_003204"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HCS1953881.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEERLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVADIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWKYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCSDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(4316..4957)
                     /gene="kdgA"
                     /locus_tag="OQQ48_003205"
                     /gene_synonym="eda"
     CDS             complement(4316..4957)
                     /gene="kdgA"
                     /locus_tag="OQQ48_003205"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HCS1953882.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(4994..6805)
                     /gene="edd"
                     /locus_tag="OQQ48_003206"
     CDS             complement(4994..6805)
                     /gene="edd"
                     /locus_tag="OQQ48_003206"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HCS1953883.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQSPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(7040..8515)
                     /gene="zwf"
                     /locus_tag="OQQ48_003207"
     CDS             complement(7040..8515)
                     /gene="zwf"
                     /locus_tag="OQQ48_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCS1953884.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            8853..9722
                     /gene="hexR"
                     /locus_tag="OQQ48_003208"
                     /gene_synonym="yebK"
     CDS             8853..9722
                     /gene="hexR"
                     /locus_tag="OQQ48_003208"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HCS1953885.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAAMALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            9850..11292
                     /gene="pyk"
                     /locus_tag="OQQ48_003209"
     CDS             9850..11292
                     /gene="pyk"
                     /locus_tag="OQQ48_003209"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCS1953886.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(11352..11570)
                     /locus_tag="OQQ48_003210"
     CDS             complement(11352..11570)
                     /locus_tag="OQQ48_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001499009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1953887.1"
                     /translation="MEYQADYYITINDSIKTWVQTQYSKDSGLPMAVIGHSVAEEAGM
                     RRLASYLDLHSGYPCIHFTGGCDYDWIE"
BASE COUNT         2823 a         2994 c         2902 g         2910 t
ORIGIN      
        1 ctgctcaaaa aagaacaaat ggtagtgtcc gctattgcca gtacacctca accagccagt
       61 cggtaaacag cgtaaaccct tccagcatga tgttttcgcg tggcggaatt aactcttccc
      121 actgttgctc atcacgcagg cgggtacggt ataagccaaa gtttttgccg tggcggttgg
      181 aacgcagata aaaccgatgc tggtagtgat caaggctgta ttcgtgatct ttgcggcgcg
      241 gcagaaaaac aaacggctcg gcatcggcca tttccgcgtc cagcaggcga acttcactgg
      301 tggtggcgct ggccaaatga atgactacat agtgcttcga ggtcgtttta tgcaggctga
      361 cgtaataggt atcgtctttt tcttcgtaga tcagtttatc ttgcgatgct ggcgtaccga
      421 tggcgtgacg ccagacctga taaggcagca gcgtcaccgg atgcttgcga acatagtaga
      481 aagtccagga gtcatttgcc cagacaaagc tgggttcaac gttatccagc agttccgggt
      541 accagttacc agtttccaga ttacgaaaac gaatgccgta ctggcgtcgg gaaagaaaat
      601 cttctgccag cgccataatg gtgttatcgg gcgtaatcgc cattccgccc atcgaataaa
      661 actcactatg agctgcgcgc ttattggcat cgagcaatgt ttcccactca tcccactctt
      721 cactgaatgc cgattgacgc tggtagatag catattcaca gcctggttca taaatatgcc
      781 gatagcggta gccatttttg atgtagggcg cagaaacttc tcgttgcgga atgcggtcga
      841 tgatttcttt taagatgcga tcctgcaagg cttgttgtga ggccatcacc cgatgaccgt
      901 aactattttc ttgttgcagg tagtccagga cttctggctg agaacgcgta tcgtcccgca
      961 gccagtagta attatcgatg cgcgtatcgc catgaagcgt catggcgtgg ggaatgcggg
     1021 cggcttttgg tagcatgtta ttgttctttc tggttgaaac atcttataag ggtggcaaaa
     1081 ctcaccgggg atgcaagcga aacaggggag tcattgctta gatgatgaca ggtaatggcg
     1141 cggatatcga atgttatgca gacagagaaa atcagcctgt tcaaatggct gtgcgattct
     1201 ggatagcccg aaatagtcaa cttcaggcta tccagagagc ggaattattc cgccaaagtg
     1261 cgtttttgct gttcgagatc gcgttcaatg ccgtcacgaa catcctgggg gattttcagc
     1321 gcgtcaccca gtgcattcag gtaactgcgt tccataaaat ggtcaatatc aatagccgcg
     1381 caactcagga aatagatttc cagcgcctct tcttcattgc ggaccccggt agccaagcgt
     1441 tgtggatcca gcggttgttc gattgcctgc tcaatgagta cacgcccctg ctcttccacg
     1501 ccggcttcac gcaattgctg gtcgattgcc gcacgttctt tggcatcaat atgaccatca
     1561 cttttagcgg caaagaccag cgcaaggatc aaacgttctg tacgctcatc cagcggcgta
     1621 ctttgcgcgc caaactgcgg ttcgtcctga tgcgccgcgc gaattttatc tttgtattta
     1681 ttccacagca ccgtacccgc taccgctccg ccgccaacca gtaacgcgtt ggtgccatat
     1741 ttcgtaagaa gtttacgtgc tgatttattt gcgaccagca gcccagccag tccgcctaat
     1801 gcgcctggca ctaacagttt gcccaatccc tgatccgcag acgaggagat agaagaactg
     1861 ctttgcccaa gcagggattg cagttgattt aaccagttag ccatatttgc tcctcaataa
     1921 ccatttttag tgcctgtagc atagcggatg aggatgtcag aaaatgtata tcgagacaaa
     1981 agatgcgcaa ataagcgggg cggtaggccg ttattcaaag aaattcgcgc caggtaaggt
     2041 atttactgta tatcaggtga taagggtatt ttaggtgaaa gtagtttgtc gttttgagtg
     2101 gcattgtttg atgctgattg cccgatgcga cgctgttgcg tcttctcagg cctacgattg
     2161 tggtgcgttc tggggcggac aaggcactcg cgccgcatcc gccaacggtc aatcgaatta
     2221 tcagaagcca ttaacgccgc tgatattccg ccattcccgc tttgcagtcc acgctgacct
     2281 ggtaatgaat atcggcactt ttaccacgca cggttagcgg tactgaccat ttatcatctt
     2341 taccctgaat gtcctgcaaa ctgacccaag ccacgggatc ggcctgaccg acaatttttt
     2401 gatcatctgc ccaacgcgcc acgcgatttt gttgataatc acgttttacg ctcgcggcaa
     2461 ttccggcagc atccagacct tcacactttg ggaaagtgac cgacttgctg gtttcattat
     2521 tggcagcgaa aactgatgcg caggcagaaa ccaacaacag ccctaaaaac gcccctcttt
     2581 ttttcatgtt tttctccata gcacaatgat tcaggagaaa gcatggtaca aattgtcagg
     2641 agcgcaagtt gcttcaggcc gcgtgtgagg catcttcctc ttcggattca gcaccgacga
     2701 cctgtgggtg gggacggtaa tttgccggtt ttcagaatgg tgctcagcac atctttttgt
     2761 tctgccagcc atagcgaaag ggcttcacgt tgctcgtctt ccatttgcac tggagaatta
     2821 gtcagccagg tgtcaagcag atccgccgta tcaagcattt tgtcataagc atcggcttcc
     2881 tttttgctgg taaacgacat tttctcttcg ccctcacgaa tgactacgta tttaacttca
     2941 accgccattt gcagcctctc ataataactg tgattttata cagtatattt cttttcggtt
     3001 gagaaatcaa aatcaacaat aaagacacac gcaaacgttt tcgtttatac tgcgcgcgga
     3061 attaatcagg ggatattcgt tatgacgtta ttaggcactg cgctgcgtcc ggcagcaact
     3121 cgcgtgatgt tattaggctc cggtgaactg ggtaaagaag tggcaatcga gtgtcagcgt
     3181 ctcggcgtag aggtgattgc cgtcgatcgc tatgccgacg caccagccat gcatgtcgcg
     3241 catcgctccc atgtcattaa tatgcttgat ggtgatgcat tacgccgtgt ggttgaactg
     3301 gaaaaaccac attatatcgt gccggagatc gaagctattg ccaccgatat gctgatccaa
     3361 cttgaagagg aaagactgaa tgttgtcccc tgcgctcgcg caacgaaatt aacgatgaat
     3421 cgcgagggta tccgtcgcct agcggcagaa gagctgcagc tgcccacttc cacttatcgt
     3481 tttgccgata gcgaaagcct tttccgcgag gcggttgctg acattggcta tccctgcatt
     3541 gtaaaaccgg tgatgagctc ttccggcaag gggcagacgt ttattcgttc tgcagagcaa
     3601 cttgctcagg catggaagta cgctcagcaa ggcggtcgcg ccggagcggg ccgcgtaatt
     3661 gttgaaggcg tcgttaagtt tgacttcgaa attaccttgc taaccgtcag cgcggtggat
     3721 ggcgtccatt tctgtgcacc ggtaggtcat cgccaggaag atggcgacta ccgtgaatcc
     3781 tggcaaccac agcaaatgag cccgcttgcc cttgaacgtg cgcaggagat tgcccgtaaa
     3841 gtggtgctgg cactgggcgg ttatggtctg tttggtgtcg agctgtttgt ctgtagtgat
     3901 gaggtgattt tcagtgaggt ctcccctcgt ccacatgata ccgggatggt gacgttaatt
     3961 tctcaagatc tttcagagtt tgccctgcat gtacgtgcct tcctcggact tccggttggc
     4021 gggatccgtc agtatggtcc tgcagcttct gccgttattc tgccacaact gaccagtcag
     4081 aatgtcacgt ttgataatgt gcagaatgcc gtaggcgcag atttgcagat tcgtttattt
     4141 ggtaagccgg aaattgatgg cagccgtcgt ctgggtgttg cgctggcgac cgctgagagt
     4201 gttgttgacg ccattgaacg cgcgaagcac gccgccggac aggtaaaagt acagggttaa
     4261 acccggcaaa aaaacgctac aaaaatgccc gatcgtagat cgggcgtttt gacttttaca
     4321 gcttagcgcc ttctacagct tcacgcgcca gcttagtaat gcggtcgtaa tcgcccgctt
     4381 ccagcgcatc tgccggaacc agccaggaac caccgatgca cagcacgctt ttcagcgcca
     4441 ggtagtcacg gtagttagcc ggagaaatac cacccgtcgg gcagaaacgg acctgggaga
     4501 acggacccgc gatcgcctgc agggctttca cgccgccgtt agcttcagcc gggaagaatt
     4561 tgaactcttt caaaccgtag tccataccca gcatcagttc ggaaacagtg ctgatccccg
     4621 gaattagagg aatagtccct tcggtagcag ctttcagcag cggctcggtc agacccgggc
     4681 taattgcgaa ctgtgcaccc gcttcagtga cttctgccag ctgctgtgga ttcagcaccg
     4741 taccggcacc cacaatcgct tcaggcactt ctttggcgat agcacggata gcgtcaactg
     4801 cacactcggt acgcagagtc acttccagaa cgcgcacccc accagcaacc aacgcttttg
     4861 ccatcggcac tgcgtgttcc agttttttta ccacgataac cggtacaacc gggccggtgg
     4921 tcaggattga ttctgcactt gttttccagt ttttcatcag agttttctct cgcctgatta
     4981 caaatttgtc gtcttaaaaa gtgatacagg ttgcgccctg ttcggcaccg gacagttttt
     5041 cacgcaaggc gctgaataat tcacgtcctg tccccacgcg tgacgcgctc aggtcaggaa
     5101 tgtgcggttc gcgagcagcc agttccgctt cgtctaccag cagcgtcagt tcgcctgtct
     5161 gtccattcac acgaatgatg tccccgtcgc gcacttttgc cagcagcccg ccatcgtagg
     5221 cttctggtgt tacgtggata gctgacggca ctttacctga agcgccggag agtcgtccat
     5281 cggtaactaa cgcaattttg aaacaccggt ccaataatac accaagtggc ggcatgagtt
     5341 tatgtaattc tggcattccg ttcgcttttg gcccctgatg acggacaaca acgacacaat
     5401 cgcggtccag caaacccgct tcaaaggccg gcataacgtc atgctggctt tcaaaaacaa
     5461 ccgctggcgc ttcaatcacc tggttctcaa ccggcacggc agaggttttc ataaccgcac
     5521 ggcccaggtt accgcttaac acttttgtcc caccatgatg agagaaaggt tgttcgaagg
     5581 aagcgatcac attgctgtcg agtgattttt ccgccccttc ccgccagtcc agttcaccat
     5641 tattcagcca tggttcaagg gtataacgag acagaccaaa gcctgccacc gtattgacat
     5701 cttcatgcag caggcctgct ttgagcagtt cacgcaccag aaccggtacg ccacctgccg
     5761 cctggaagtg gttaatatcg gccggaccgt tcgggtagag acgtgccatc agcggtacaa
     5821 catcagaaag gtcagagaag tcatcccagt taatctgaat accggccgcg cgcgccatcg
     5881 ccaccaggtg catggtgtgg ttagtggaac caccggtcgc cagcagtgca acgataccgt
     5941 tcaccaccac tttctcatcg atcatcttac cgatcggcat ccattcatta ccattaccgg
     6001 tcatgcgtgt aacctgacgc gcagcggcag cggtcaaagc atcgcgcagc ggagaatccg
     6061 gatgaacaaa agaagagcct ggcgactgca tccccataaa ttccaccacc atctggttgg
     6121 tgttggcagt accgtagaaa gtacatgttc ccggcgcatg gtaagacgcg gcttctgact
     6181 ccagtaaggc catgcggtcc actttacctt cggcataaag ctggcgaata cgcacttttt
     6241 ctttatttgg caaaccgctt gccatcggtc cagacggcac aaacaccgca ggcaaatgac
     6301 caaacgacag ggctgccatc gtcagacccg ggacaatctt gtcgcacaca ccgaggaaca
     6361 gagcaccatc aaacatgtta tgggacagcc ccaccgccgc agacatcgct atcacttcgc
     6421 ggcttagcag cgacaattcc attccatcct gcccctgggt gacaccatca cacatcgccg
     6481 gaacaccgcc cgcaacctga ccaaccgcat tcgcttcatg cagggcttta cgaatgattt
     6541 ctggatagtg ttcataaggc tggtgcgcgg agagcatgtc gttataggag gtgatgatgg
     6601 cgatattgtt acgcaacatg cttttcaaag aggctttgtc ttctggctgg caggcagcga
     6661 aaccgtgtgc caggttaccg catgccaact gcgaacgatg aacggtcgac gttttcgctt
     6721 gttctatccg ggcgagataa gcagagcgag tctcgcgcga acgttcaatg attcgatttg
     6781 ttacgcgtaa caattgtgga ttcataaagg ctcctgaaat tgagttgtca gagcaggatg
     6841 attcacaacg cgtttcattc agaggattta tgagtgaaac gcctgtaacc ggagctcata
     6901 gggcaaaaac gtttcagtca gtgtaataaa aaaagcctcg tgggtgaatc cgcacgaggc
     6961 ctgaaagtgt aaaaattgtt ctacaatctg tgcaagatca tgttaccggt aaaataacca
     7021 taaaggataa gcgcagatat tactcaaact cattccagga acgaccatca cgggtaatca
     7081 tcgccaccga ggcaacgggt ccccaggttc cggcctgata cggtttcggc gcatcattgt
     7141 ccatcgccca cgcctcagta atggagtcta cccatttcca ggcttcttcc acttcatcac
     7201 gacgtacaaa cagtgcctga ataccacgca tggtttccag cagcagacgt tcataggcat
     7261 ccgccagatg cgtctgatta aaggtttctg aatagctcag atccagcttg gtgatttgca
     7321 ggttatgttt gtggtcaagg ccaggaactt tattcagtac ctggatatcc acgccttcat
     7381 caggttgcag acggatagtc agtttattct gcggcagatc ctgccacgac tctttaaaca
     7441 gattcagttc aggtgttttg aaatagacca cgacttcaga acatttggtc ggcagacgtt
     7501 taccagtacg caggtagaat ggcacaccgg cccagcgcca gttatcaatg tcgacgcgga
     7561 tcgccacgaa ggtttctgta ttgctgctct tgttcgcacc ctcttcttcc agatatcccg
     7621 gcactttttt gccctgggcg aagcccgcag tatattgccc acgtacggtt ttttcgcgta
     7681 cgttggagcg gtcgatgcgg cgcagggact tcagtacttt cactttttca tcgcggatgc
     7741 tgtctgcgct caggtcagat ggcggagaca tcgcaatcat gcagagaatt tgtagcaggt
     7801 ggttctgaat catgtcacgc atctgaccgg ctttatcaaa atagccccag cgcccttcga
     7861 tccccacttc ttctgccacg gtaatctcaa catgatcaat ggtgcgattg tcccagttat
     7921 tcacaaacag ggagttagca aaacgcagcg ccaacaggtt cagcaccgtt tctttaccaa
     7981 gatagtggtc gatacggtaa acctggcact cctcgaagta ttcgccaacc tgatcattga
     8041 tttcctgcga ggtcgccagc gacgtcccca gcggtttctc catgactacg cgtgccggtt
     8101 tagcattcag ttttgcctcg ccaagccctt tgcaaattgc gccaaaagtg ctgggcggca
     8161 tggcaaagta gttaatggtg atacgatttt tttgatccag catcgcgccg agacggttga
     8221 atgcagcagt gtcattgaca tcgagattac aaaaatccag acgtgcactc agggtgtccc
     8281 ataaaccttc atcaatggtt tctttcatga aagtttcgag cgcctcgcgg acaactttgg
     8341 tatatgccgc tttatcccag tcagcacgcc ctacgccgat aatccgggtg tccgggttga
     8401 gctgaccggc tttttccagt tgatacaggg aaggcagcaa tttacgacgc gcaaggtcgc
     8461 ctttcgcgcc gaaaatgacc aggtcacagg cctgggctgt ttgcgttacc gccatgtcat
     8521 tctccttaag ttaactaacc cggtacttaa gccagggtat acttgtaatt ttcttacggt
     8581 gcactgtact gcttttacga gcttgcgaaa actgtaaacg cttatccacc cgtgcgatta
     8641 cgggaaaagc gcgcaaagcg cggcaaaact gataaaaaaa tcatcgtttt ccattcggta
     8701 aaaatctgac actgatcatg ttatgaaaaa aaataacaac ttttttatct gcttttgtca
     8761 ttaacacggc acacaggcgt aatatctgac aaaactgcat ttcgatttct ttcagtgcgg
     8821 aaatcgtcat tacccgtgag tctctttaca tcatgaatat gctggaaaaa atccagtctc
     8881 agctggaaca tttgagcaaa tcagagcgca aagttgccga ggtcattctg gcttcgcccg
     8941 ataacgcgat ccattcgagt attgctgcta tggcactgga agccaatgtt agcgaaccga
     9001 cggtgaatcg tttctgtcgc agcatggaca cgcgcggttt tcctgatttt aaacttcatc
     9061 tggcacagag tctggcgaat ggcactccct atgttaatcg caatgtcaat gaagatgaca
     9121 gcgttgaatc atacacaggg aaaatatttg agtccgcaat ggcaacgctt gatcatgtcc
     9181 gtcattcact ggataaatct gccatcaacc gcgccgtcga cttgctcact caggcaaaaa
     9241 aaatcgcctt tttcggatta ggctcttcag ccgccgttgc ccacgatgcg atgaataagt
     9301 tctttcgttt taatgttccg gtggtgtact ccgatgatat cgtgctgcaa cgcatgagtt
     9361 gtatgaattg tagcgacgga gacgtggtgg tgctgatttc tcacactgga agaacaaaaa
     9421 atctggtcga gctggcgcag ctggctcgcg aaaacgacgc aatggtgatt gccctcacct
     9481 ctgcgggtac cccgctcgcc cgggaagcaa cgctggcaat taccctcgac gtaccggaag
     9541 atactgacat ttatatgccc atggtttctc gacttgcaca gctgaccgtg atagatgtgc
     9601 tggcgacagg atttactttg cgacgcggtg caaaattcag agataacttg aagcgggtca
     9661 aggaagcgct gaaggaatcg cgttttgata agcagttact taatttaagt gacgatcgct
     9721 aaaaacgact gtcactgtcc taatcttata cgacatccga atgagattaa tttatcgcca
     9781 tcgcggcgtt atttcattcg gatttcatgt tcaagcaaca cctggttgtt tcagtcaacg
     9841 gagtattaca tgtccagaag gcttcgcaga acaaaaatcg ttaccacgtt aggcccagca
     9901 acagatcgcg ataataatct tgaaaaagtt atcgcggcgg gtgccaacgt tgtacgtatg
     9961 aacttttctc acggctcgcc tgaagatcac aaaatgcgcg cggataaagt tcgtgagatt
    10021 gccgcaaaac tggggcgtca tgtggctatt ctgggtgacc tccaggggcc caaaatccgt
    10081 gtatccacct ttaaagaagg caaagttttc ctcaatattg gggataaatt cctgctcgac
    10141 gccaacctgg gtaaaggtga aggcgacaaa gaaaaagtcg gtatcgacta caaaggcctg
    10201 cctgctgacg tcgtgcctgg tgacatcctg ctgctggacg atggtcgcgt ccagttaaaa
    10261 gtactggaag ttcagggcat gaaagtgttc accgaagtca ccgtcggtgg tcccctctcc
    10321 aacaataaag gtatcaacaa acttggcggc ggtttgtcgg ctgaagcgct gaccgaaaaa
    10381 gacaaagcag acattaagac tgcggcgttg attggcgtag attacctggc tgtctccttc
    10441 ccacgctgtg gcgaagatct gaactatgcc cgtcgcctgg cacgcgatgc aggatgtgat
    10501 gcgaaaattg ttgccaaggt tgaacgtgcg gaagccgttt gcagccagga tgcaatggat
    10561 gacatcatcc tcgcctctga cgtggtaatg gttgcacgtg gcgacctcgg tgtggaaatt
    10621 ggcgacccgg aactggtcgg cattcagaaa gcgttgatcc gtcgtgcgcg tcagctaaac
    10681 cgagcggtaa tcacggcgac ccagatgatg gagtcaatga ttactaaccc gatgccgacg
    10741 cgtgcagaag tcatggacgt agcaaacgcc gttctggatg gtactgacgc tgtgatgctg
    10801 tctgcagaaa ctgccgcagg gcagtatccg tcagaaactg ttgcagccat ggcgcgcgtt
    10861 tgcctgggtg cggaaaaaat cccgagcatc aacgtttcta aacaccgtct ggacgttcag
    10921 ttcgacaatg tggaagaagc tattgccatg tcagcaatgt acgcagctaa ccacctgaaa
    10981 ggcgttacgg cgattattac catgaccgaa tcgggtcgta ccgcgctgat gacctcccgt
    11041 atcagctctg gtctaccaat tttcgccatg tcgcgccatg aacgtacgct gaacctgact
    11101 gctctctatc gtggcgttac gccggtgcac tttgatagcg ctaatgacgg cgtagcagct
    11161 gccagcgaag cggttaatct gctgcgcgat aaaggttact tgatgtctgg tgacctggtg
    11221 attgtcaccc agggcgacgt gatgagtacc gtgggttcta ctaataccac gcgtatttta
    11281 acggtagagt aatctcacct gccccggccg ctgatggccg gggtatttag caacacataa
    11341 attaggacta cttattcaat ccaatcataa tcacagcccc ctgtaaaatg aatacaggga
    11401 tagcctgaat ggagatctaa atagcttgca agacgtcgca tacccgcttc ctctgcaaca
    11461 ctatgtccaa taactgccat cggtaagcca ctatctttgg agtattgtgt ctgcacccat
    11521 gttttaattg aatcgttaat cgtaatataa taatcagcct gatattccat atattgtatt
    11581 ggatcacaaa aacagcctgc cccaatgcca acagttctga ctactctgg
//
LOCUS       DAJVUJ010000134        11527 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.134, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000134 DAJVUJ010000000
VERSION     DAJVUJ010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11527
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.134"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(<1..206)
                     /locus_tag="OQQ48_003211"
     CDS             complement(<1..206)
                     /locus_tag="OQQ48_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCS1953888.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYVLTTQVEFTDPEIV"
     gene            362..1402
                     /gene="gpr"
                     /locus_tag="OQQ48_003212"
     CDS             362..1402
                     /gene="gpr"
                     /locus_tag="OQQ48_003212"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HCS1953889.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGVSRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(1442..1936)
                     /locus_tag="OQQ48_003213"
     CDS             complement(1442..1936)
                     /locus_tag="OQQ48_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HCS1953890.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            2127..3011
                     /gene="yghA"
                     /locus_tag="OQQ48_003214"
     CDS             2127..3011
                     /gene="yghA"
                     /locus_tag="OQQ48_003214"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCS1953891.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGQAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQQTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(3283..3708)
                     /gene="exbD"
                     /locus_tag="OQQ48_003215"
     CDS             complement(3283..3708)
                     /gene="exbD"
                     /locus_tag="OQQ48_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HCS1953892.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(3715..4449)
                     /gene="exbB"
                     /locus_tag="OQQ48_003216"
     CDS             complement(3715..4449)
                     /gene="exbB"
                     /locus_tag="OQQ48_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCS1953893.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLFNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            4701..5888
                     /gene="metC"
                     /locus_tag="OQQ48_003217"
     CDS             4701..5888
                     /gene="metC"
                     /locus_tag="OQQ48_003217"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HCS1953894.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLSNEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            6028..6687
                     /locus_tag="OQQ48_003218"
     CDS             6028..6687
                     /locus_tag="OQQ48_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCS1953895.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGMLFVVIKKKYCN
                     A"
     gene            complement(6727..7626)
                     /gene="yqhC"
                     /locus_tag="OQQ48_003219"
     CDS             complement(6727..7626)
                     /gene="yqhC"
                     /locus_tag="OQQ48_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HCS1953896.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            7820..8983
                     /gene="yqhD"
                     /locus_tag="OQQ48_003220"
     CDS             7820..8983
                     /gene="yqhD"
                     /locus_tag="OQQ48_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HCS1953897.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAADNYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRDTKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            9088..9915
                     /gene="dkgA"
                     /locus_tag="OQQ48_003221"
     CDS             9088..9915
                     /gene="dkgA"
                     /locus_tag="OQQ48_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HCS1953898.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVIIAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            10115..11041
                     /locus_tag="OQQ48_003222"
     CDS             10115..11041
                     /locus_tag="OQQ48_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HCS1953899.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            11092..11349
                     /gene="yqhH"
                     /locus_tag="OQQ48_003223"
     CDS             11092..11349
                     /gene="yqhH"
                     /locus_tag="OQQ48_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HCS1953900.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
BASE COUNT         2980 a         2919 c         2890 g         2738 t
ORIGIN      
        1 acaatctccg ggtcggtaaa ctctacctga gtcgtcagca cataatttgg cttgagcaaa
       61 tcaaacaacg ctaatgccgt catcccgccc accgcatact tcgccgcaag gttgaggaac
      121 tcacgtttcg aaattttccc gtgagcgtaa tagtcgtagt aatccaacaa ctcttgtggg
      181 aaatctttgg cggttaaacg cggcatcgtt gcattcctca actggtgttt tttaagcaaa
      241 gcataagcgc gtgtttttgc ccagtttttc gtcactctgt gagccagact acgggatacg
      301 cgctggcgaa tcgctaaact agaaacattg tttcgaaatt gaacggtgga aaggagaggt
      361 catggtctgg ttagcgaatc ccgaacgtta cgggcagatg cagtaccgct attgcggaaa
      421 aagtggttta cgcctgcccg cgttatcgct cggtttatgg cacaatttcg gtcacgttaa
      481 cgcgctggaa tcacagcgtg caatcctgcg taaagcgttt gatttaggca ttacgcactt
      541 tgatttagcc aacaattacg ggccgcctcc aggaagcgca gaagagaact ttggtcgcct
      601 gctgcgggag gattttgccg cttatcgcga tgaactgatt atctctacca aggctggcta
      661 cgatatgtgg cccggccctt acggctctgg cggttcacgt aaatacctgc tcgccagcct
      721 cgaccaaagc ctgaagcgta tggggcttga gtatgtcgat atcttttact ctcatcgcgt
      781 cgatgaaaat acgccgatgg aagaaaccgc ctctgcgctg gctcatgcgg tacaaagcgg
      841 taaggcgctg tatgtcggga tctcctctta ctcgccagag cggacgcaaa aaatggtcga
      901 gttgctgcac gagtggaaaa ttccgctttt aattcatcaa ccttcgtaca atttactgaa
      961 ccgctgggtg gataaaagcg gcctgctgga taccctgcaa aataacggcg tgggctgtat
     1021 tgcctttact cctctggctc agggattgct gaccggaaaa tatctcaacg gcattccgca
     1081 agattcacgg atgcatcgtg aagggaataa agttcgtggt ctgacgccga aaatgcttac
     1141 cgaagccaac ctcaacagcc tgcgcttatt gaatgaaatg gcacagcagc gtggacaatc
     1201 aatggcgcaa atggcgttaa gctggttgct gaaagatgat cgcgtgacgt cggtattgat
     1261 tggtgtcagc cgcgcggagc aactagagga gaacgtgcag gcgctgaata atctgacatt
     1321 tagcaccgag gagctggcgc agattgatca gcatatcgcc gatggcgagc tgaatctgtg
     1381 gcaggcgtct tccgataaat gacctgttaa taacgggcgc gcgaaccgcg cccgaatcag
     1441 atcagtgatt atgacgagtc agtcggtcaa gatagcccat cacaaatgca gagagcacaa
     1501 acgtcagatg gataatgacg taccacatca gtttgttatc cgggacattt ttcgcatcca
     1561 taaagacgcg cagtaagtga atggaagaaa ttgccacaat cgacgctgct actttgtttt
     1621 tcagcgacgt tgcgtccatt ttccccagcc agttcagctt ctctttgttc tcggagatat
     1681 ccagctgcga gacgaaattc tcataaccgg aaaacatcac catcaccagc aaaccgccaa
     1741 ccagcgtcat atccaccagc gacagcaaca cgaggatcaa atctgattcc gccatcgaga
     1801 agatattcgg cagtacgtga ataatctcct ggaagaactt cagcgccagg gcaactaacg
     1861 ccagcgaaag gccaaagtac acgggggcaa gcagccagcg agaagcatac attgcatttt
     1921 caagaaaacg ttccatacat tcctgtcgtg tagttaaaac gggcaacagt atatcgcaat
     1981 tgagtaaaca gcacgcaaca gcaaatcaac atatacgtta aacctttgtc gcataattct
     2041 gaggtttagg gatcttcctg gttcccttat caacgcatcg agagcgtcta cccttaaaag
     2101 acgttgaacc tgaagggaga aaaacaatgt ctcatttaaa agacccgact acgcagtatt
     2161 acactggtga atatcccaaa cagaaacaac cgacgccagg catccaggcg aagatgacac
     2221 cggtaccgga ttgcggcgag aaaacctatg ttggtagcgg tcgcctgaaa gatcgtaaag
     2281 cactggtgac agggggcgat tccggaatag gtcgcgctgc cgccatcgct tacgcgcgtg
     2341 aaggggctga cgtggcgatc agttatcttc ccgtggaaga agaagacgct caggatgtga
     2401 aaaagatcat tgaagaatgc ggacgcaaag ccgttctgct gccaggcgat ttaagcgatg
     2461 agaaattcgc ccgttcgctg gttcacgaag cgcacaaggc gttaggcggg ctggatatta
     2521 tggcgctggt cgccgggaaa caggttgcca ttccggatat tgcagacctc accagcgaac
     2581 agtttcaaaa gacctttgcc attaacgttt tcgcgctgtt ctggctaacc caggaagcga
     2641 tccccctgct accgaaaggt gcaagtatca tcaccacttc gtcaatccag gcataccagc
     2701 caagtccgca tttactggac tatgcggcta cgaaggcggc gattctgaac tacagccgtg
     2761 gccaggcaaa acaggtcgcg gagaaaggta ttcgggtgaa tattgtcgcg ccaggcccta
     2821 tctggacggc actgcaaatt tccggcggac aaacgcagga taagatcccg cagtttggtc
     2881 agcaaacgcc gatgaagcgt gcggggcaac cggcggaact ggcccctgta tatgtttatc
     2941 tggcaagtca ggagtcgagc tacgtcaccg cagaagtgca cggtgtgtgc ggcggcgagc
     3001 atttaggtta aaaaaatgcc cggttgtgaa aagcaaccgg gcatcattgt gaattcactc
     3061 ttacctgatg tggcgaaaac gccttatccg cctacaaacg catgaagatt caacatgttg
     3121 caatagattt tgtaggcctg ataagcgtag cgcatcaggc attgagcacc taatgccgga
     3181 tgcggcgtga acgccttatc cggcctacaa acgcacgaag attcaacatg ttgcaataga
     3241 ttttgtaggc ctgatgagcg tagcgcatca ggcaattcta ctttacttcg ctttggcggt
     3301 ttcttcgccg accagaccta tcttcaggta acccgcctga tgcagcgtat ccattacctt
     3361 catcaacgtc tcgtaatcga cggttttatc cgctcggaag aagatggtgg tgtctttctt
     3421 gccttcggtt aacgcattca acgccgtaat cattgtttca tcggtgaccg gatcgttacc
     3481 gataaacatc gagttgtctg ccttcaccga cagataaacc ggtttttccg ggcgcggctg
     3541 cggcgtgctg gtagaagcag gcaagttcac cttcacatct accgtcgcta acggtgccgc
     3601 caccataaag ataatcagca gaaccaacat cacgtcgata aacggcgtca cgttgatatc
     3661 atgcatttcg ccgttatcgt cgaggttttc gttaagatgc attgccatcg gatattatcc
     3721 tgcgcgtaat ttttgtgcga cacgaaccgg atgcgcagcg gcgctggctt ccagatccag
     3781 gtcacggctt tgcagcaaca atacctgcgc tgcaacatca cccagcatcg ctttaaagcc
     3841 gccaatctgg cgtgcaaata cgttatagat aacgaccgca ggaatcgctg ccacgaggcc
     3901 gattgccgtt gctaacagag cttctgcgat acccggcgca acgactgcca ggttagtggt
     3961 ctgcgtttgc gcgataccaa taaagctgtt catgatgccc cagaccgtac caaacagacc
     4021 aacgaacgga gaaatcgcgc cgatggttgc cagatagccg ttaccgcggc ccatttgacg
     4081 acccactgcg gcgacccgac gctccagacg gaagctggta cgttctttaa taccttcgtt
     4141 atcgtcgctg ccttctgaca gttccagctc attctgcgct tcattgaaca aatgcaggct
     4201 taagcttttg ctaccaaaat cagcggcgat atcgttggcc tggtttaagg aacgcgcttc
     4261 agccagcagt tgctgctcgc gctttaggcg acgcttctga ttgaagaact ctacgctctt
     4321 actgaagaag attgcccagg tgactacgga ggccaaaata agcccaatca tcacgcactt
     4381 aacgacaata tcggcgtgct gatacatacc ccagacggaa aggtctgtct gcattaaatt
     4441 attacccact gtgtatctcc aggacgcaag tcacaaaatc tgcgcataat aatatcaaaa
     4501 cgacgtcgaa ttgatagtca ttctcattac tatttgcata ctgccgtacc tttgctttct
     4561 tttccttgcg tttacgcagt aaaaaagtca ccagcacgcc atttgcgaaa attttctgct
     4621 ttatgccaat tcttcaggat gcgcccgcga atattcatgc tagtttagac atccagacgt
     4681 ataaaaacag gaatcccgac atggcggaca aaaagcttga tactcaactg gtgaatgcag
     4741 gacgcagcaa aaaatacact cttggcgcgg taaatagcgt gattcagcgc gcttcttcgc
     4801 tggtatttga gagtatggaa gccaaaaagc acgcgacgcg caatcgcgcc aatggcgagt
     4861 tgttctatgg acggcgcgga acgttaaccc atttttcctt acaacaagcg atgtgtgaac
     4921 tggaaggtgg cgcaggctgc gcgctatttc cctgcggggc ggcagcggtt gctaattcga
     4981 ttcttgcttt tgtcgaacag ggcgatcatg tgttgatgac caacactgcc tatgaaccga
     5041 gtcaggattt ctgtagcaaa atcctcagca aactgggcgt aacgacatcg tggtttgatc
     5101 cgctgattgg tgccgatatc gttaagcatc tgcagccaaa cactaaaatc gtgtttctgg
     5161 aatcgccagg ctccatcacc atggaagtcc acgacgttcc ggcgattgtt gccgccgtac
     5221 gcagtgtggt gccggatgcc atcattatga tcgacaacac ctgggcagcc ggtgtgctgt
     5281 ttaaggcact ggattttggc atcgatgttt ctattcaagc cgccaccaaa tatctggttg
     5341 ggcattcaga tgcgatgatt ggcactgccg tgtgcaatgc ccgttgctgg gagcagctac
     5401 gggaaaacgc ctatctgatg ggccagatgg tcgatgccga taccgcctat ataaccagcc
     5461 gtggcctgcg cacattaggt gtgcgtttgc gtcaacatca tgaaagcagt ctgaaagtgg
     5521 ctgaatggct ggcagaacat ccgcaagtag cgcgagttaa ccaccctgct ctgcctggca
     5581 gtaaaggaca cgaattctgg aaacgagact ttacaggtag cagcgggctg ttttcctttg
     5641 tgcttaagaa aaaactcagt aatgaagagc tggcgaacta tctggataac ttcagtttat
     5701 tcagcatggc ctactcgtgg ggcgggtatg aatcgttgat cctggcgaat caaccagaac
     5761 atatcgccgc cattcgccca caaggcgaga tcgattttag cgggaccttg attcgcctgc
     5821 atattggtct ggaagatgtc gacgatctga ttgccgatct ggacgccggt tttgcgcgaa
     5881 ttgtataaca ttgccacttt tggacaattt tgcagacatt taattgtgaa aagtcttaaa
     5941 ttgttgcgtc cgggatcaag gcgtcccgga cgaatcagga gtacaatagg cagataaagg
     6001 cttaaacgct gttccacagg aaagtccatg gctgttattc aagatatcat cgctgcgctc
     6061 tggcaacacg actttgccgc gctggcggat cctcatattg ttagcgttgt ttactttgtc
     6121 atgtttgcca cgctgttttt agaaaacggc ctgctgcccg cctcattttt gccaggcgac
     6181 agcttgttga tactggcagg cgcattgatt gcccaggggg ttatggattt tctgcctacg
     6241 attgcgattc tgaccgccgc agcaagtctg ggctgctggc taagttatat tcaggggcgc
     6301 tggttaggga ataccaaaac ggtgaaaggc tggctggcac agcttcctgc taaatatcac
     6361 cagcgcgcca cctgcatgtt tgaccgccac ggtctgctgg cgctgctggc tggacgtttt
     6421 cttgcatttg tccgtacgct gctgccaacc atggcgggaa tttccggtct gccaaaccgc
     6481 cgcttccagt ttttcaactg gttaagtgga ttgctgtggg tcagcgtggt aaccagtttt
     6541 ggctatgcct taagtatgat tccgttcgtt aaacgccatg aagatcaggt aatgacgttc
     6601 ctgatgatcc tgccaattgc cttgttaacc gctggcttgt taggcatgct gtttgtggtg
     6661 attaaaaaaa aatactgtaa cgcctgacga ttttccccgt tcccggctgc tgtaccggga
     6721 acgtatttaa ttcccctgca tcgcccgcat tcttgccgca tcttcccccg gcgtcacacc
     6781 gaagtaacgt ttaaactcac ggctaaattg cgatgcgctt tcatagccga cgcgcatcgc
     6841 tgctgcgctg gccttcatgc cgtcatggat gatcatcatc cgcgccttat gcagacggta
     6901 attcttcaaa tactgcaacg gcgaggtgct ggtgacagac ttaaaattat ggtggaacgc
     6961 cgatacgctc atgttggctt ctgccgccag ttgctcgacg ctcaggtttt cggtgtattt
     7021 attctcaatc cgtttcagca cgcggctaat cagactgaag tgagtctggc gactgaccag
     7081 cgccagtaac gcgccgccgc aaggtccggt cagcacgtag tacagaattt cgcggatgat
     7141 ctgtttgccg agaatacgcg catccagtgg tcgctccatc acgtcgagta accgctccgc
     7201 cgcgcataaa atctcttctg ataacgtggc ggagttaatc ccgctggctg ccatcgacgg
     7261 ctggaaatgc tcatcttcgc caatgtccat caacagttcc tgtaactgca aaatatcgac
     7321 attgagacgc aaccctgcca gcggcacctc tgacgtggca taggtttcgc actcaaacgg
     7381 caacggcacc gtcagcagca ggtattcatt ggcatcataa cgaaacacgc gttcattgat
     7441 ataaccgatt ttatgcccgg aaaagagaat tatgatgcca ggctcgtaca tcaccggtgt
     7501 acgtgcgaaa ggcgtctcac catacaacaa acgcacatcg ggcaacagtt ctgacaaact
     7561 attttcttta tttttcagtt tattaacttt atccgccagc aagcggcaaa tctcttcacg
     7621 tttcatatcg cgtaatttct taggaataat gcggcaattt gattgtgcgc aattttgtag
     7681 catttctcca gcactctgga gaaataggca agacattggc agaaatgagc attgagagcc
     7741 agggcgctgg cgatcacaat gaaaaacatc aggcagatcg ttctctgccc tcatattggc
     7801 ccagcaaagg gagcaagtaa tgaacaactt taatctgcac accccaaccc gcattctgtt
     7861 tggtaaaggc gcaatcgctg gtttacgcga acaaattcct cacgatgctc gcgtattgat
     7921 tacctacggt ggcggcagcg tgaaaaaaac cggcgttctc gatcaagttc tggatgccct
     7981 gaaaggcatg gacgtgctgg aatttggcgg tattgagcca aacccggctt atgaaacgct
     8041 gatgaacgcc gtgaaactgg ttcgcgaaca gaaagtgact ttcctgctgg cggttggcgg
     8101 cggttctgta ctggacggca ccaaatttat cgccgcagcg gataactatc cggaaaatat
     8161 cgatccgtgg cacattctgc aaacgggcgg taaagagatt aaaagcgcca tcccgatggg
     8221 ctgtgtgctg acgctgccag caaccggttc agaatccaac gcaggcgcgg tgatctcccg
     8281 taaaaccact ggcgacaagc aggcgttcca ttctgcccat gttcagccgg tatttgccgt
     8341 gctcgatccg gtttatacct acaccctgcc gccgcgtcag gtggctaacg gcgtagtgga
     8401 cgcctttgta cacaccgtgg aacagtatgt taccaaaccg gttgatgcca aaattcagga
     8461 ccgtttcgca gaaggcattt tgctgacgct gatcgaagat ggtccgaaag ccctgaaaga
     8521 gccagaaaac tacgatgtgc gcgccaacgt catgtgggcg gcgacgcagg cgctgaacgg
     8581 tttgattggc gctggcgtac cgcaggactg ggcaacgcat atgctgggcc acgaactgac
     8641 tgcgatgcac ggtctggatc acgcgcaaac actggctatc gtcctgcctg cactgtggaa
     8701 tgaaaaacgc gataccaagc gcgctaagct gctgcaatat gctgaacgcg tctggaacat
     8761 cactgaaggt tccgacgatg agcgtattga cgccgcgatt gccgcaaccc gcaatttctt
     8821 tgagcaatta ggcgtgccga cccacctctc cgactacggt ctggacggca gctccatccc
     8881 ggctttgctg aaaaaactgg aagagcacgg catgacccaa ctgggcgaaa atcatgacat
     8941 tacgttggat gtcagccgcc gtatatacga agccgcccgc taagcttttt acgcctcaaa
     9001 ctttcgtttt cgggcatttc gtccagactt aagttcacaa cacctcaccg gagcctgctc
     9061 cggtgagttc atataaagga ggaacgtatg gctaatccaa ccgttattaa gctacaggat
     9121 ggcaatgtca tgccccagct gggactgggc gtctggcaag caagtaatga ggaagtaatc
     9181 atcgccattc aaaaagcgtt agaagtgggt tatcgctcga ttgataccgc cgcggcctac
     9241 aagaacgaag aaggtgtcgg caaagccctg aaaaatgcct cagtcaacag agaagaactg
     9301 ttcatcacca ctaagctgtg gaacgacgac cacaagcgcc cccgcgaagc cctgctcgac
     9361 agcctgaaaa aactccagct tgattatatc gacctctact taatgcactg gcccgttccc
     9421 gctatcgacc attatgtcga agcatggaaa ggcatgatcg aattgcaaaa agagggatta
     9481 atcaaaagca tcggcgtgtg caacttccag atccatcacc tgcaacgcct gattgatgaa
     9541 actggcgtga cgcctgtgat aaaccagatc gaacttcatc cgctgatgca acaacgccag
     9601 ctacacgcct ggaacgcgac acacaaaatc cagaccgaat cctggagccc attagcgcaa
     9661 ggagggaaag gcgttttcga tcagaaagtc attcgcgatc tggcagataa atacggcaaa
     9721 accccggcgc agattgttat ccgctggcat ctggatagcg gcctggtggt gatcccgaaa
     9781 tcggtcacac cttcacgtat tgccgaaaac tttgatgtct gggatttccg tctcgacaaa
     9841 gacgaactcg gcgaaattgc aaaactcgat cagggcaagc gtctcggtcc cgatcctgac
     9901 cagttcggcg gctaacatgc aaattctccc ggtggcggta atgttccgct accggacttt
     9961 tcagaaatca tttattcccc tcgcgtcccg cccgttgtta ctcttccttg ttcaggaatg
    10021 ccaaatataa ggacatcatc atgcagagcc ggaagctctt aaaagaacaa ctcatctata
    10081 tccgggataa acgcaacgga gaggtgaaaa acagatgaaa ataatacttc tgtttttagc
    10141 agccctggca agttttaccg tacacgcgca gcctccttca cagaccgtag aacaaacagt
    10201 ccggcagatt tatcagaact ataaatcaga tgccagtacg ccttatttcg gtgaaaccgg
    10261 agagcgggcg ataacttcag cgcgtattca acaggcgctt accctgaacg acaatcttac
    10321 gctgccgggc aatattggct ggctggatta tgatccggtt tgtgattgtc aggattttgg
    10381 cgatctggtg ctagaaagcg ttgcgataac ccaaactgac gccgatcatg ccgatgccgt
    10441 tgtgcgcttt cgtatcttta aagatgataa agaaaagacc acgcagacac tgaaaatggt
    10501 ggcggaaaat ggtcgttggg tcattgacga tattgtcagc aatcatggca gtgtcttaca
    10561 agcagttaat agcgagaatg aaaaaacgct ggccgcttta gcttcgttgc aaaaagaaca
    10621 gccggaagcc tttgttgccg aactctttga acatattgct gattatagct ggccgtggac
    10681 gtgggtggtt tccgactctt accgccaggc ggttaatgcc ttctataaaa ccaccttcaa
    10741 gacggccaat aatcccgatg aagatatgca aatagaacgg caatttattt acgacaatcc
    10801 gatctgtttt ggcgaagagt cgctattttc acgcgttgat gaaattcgag tcctggagaa
    10861 aaccgccgat tccgcccgca ttcatgttcg ttttacgctg accaatggca acaacgaaga
    10921 gcaagaactg gttttacagc ggcgcgaagg caagtgggaa atcgctgatt ttatccgccc
    10981 gaacagcggc agcctactta agcagattga ggcaaaaact gccgccagat taaagcaatg
    11041 agctgaatta aataacaatt agccggaaca ataaataaaa gggaacacta tatgaaaacg
    11101 attttcaccg tgggagctgt tgttctggca acctgcttgc tcagtggctg cgtcaatgag
    11161 caaaaggtca atcagctggc gagcaatgtg caaacattaa atgccaaaat cgcccggctt
    11221 gagcaggata tgaaagcact acgcccacaa atctatgctg ccaaatccga agctaacaga
    11281 gccaatacgc gtcttgatgc acaggactat tttgattgcc tgcgctgctt gcgtatgtac
    11341 gcagaatgat aaaaaaatcc ccggcagcat gtcagttgcc gggttatagg ctgtttgcta
    11401 aaaaaaagcc aaacataagg acatgattat gcgacgctgg cacacctgtt tttttattct
    11461 gctgttctgt ggcttcagcg gaaacgtgtt gtgcaaagaa tcgattaatg aagcgataga
    11521 acgtttg
//
LOCUS       DAJVUJ010000135        11330 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.135, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000135 DAJVUJ010000000
VERSION     DAJVUJ010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11330
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.135"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(1..111)
                     /locus_tag="OQQ48_003224"
                     /pseudo
     CDS             complement(1..111)
                     /locus_tag="OQQ48_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            388..1263
                     /locus_tag="OQQ48_003225"
     CDS             388..1263
                     /locus_tag="OQQ48_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HCS1953901.1"
                     /translation="MRKFILISMITLASVSLGACRQNATIKQDAPGQKAFLIDGQIYD
                     LHSGKIISSSELLADLATARHLIIGEKHDNAEHHQIELWLIQNLLIQRPQGSVLLEML
                     TSDQQPRVNQVKCWLKDNPVVRDSRVQELLNWQKGWSWEMYGDIVMQLLRGPYPLLNA
                     NIGREQMLALYKKNEFPKGKKSTAPVVQEALRETIIAMHEGNLESQQLTSMLSVQQQR
                     DRYMARQLLSAPVPSLLIAGGYHASKNMGVPLHMEDLATGTHPVVLMLAEKGMNITVD
                     HADYVWFVAPDTTKR"
     gene            1271..2047
                     /locus_tag="OQQ48_003226"
     CDS             1271..2047
                     /locus_tag="OQQ48_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000981087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HCS1953902.1"
                     /translation="MMNILHFPQSVKWSSWFICSLLLHGLIFLTFIWRFSEVQPAMSP
                     APAIMLQWAEIIEAPSSPLSLPVGIAQQESAVTEEKQQTEDRQQRPVTEDSDATIEIT
                     RKKKSSDGEKKKTRPPRKIKAQTSDSNPTAVSSNAAPQALVESSRIAAPFNSDSTKRD
                     NSEASWESRVKGHLNRYKRYPGDARKRARTGTAVVTFTVNTEGTIVSSFLEISSGTLS
                     LDREAIAVLERAQPLPKPPPEILEGGLFKVKMPITFKLKE"
     gene            2216..4477
                     /locus_tag="OQQ48_003227"
     CDS             2216..4477
                     /locus_tag="OQQ48_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCS1953903.1"
                     /translation="MNVIKLAIGSGILLLSCGAYSQSISEKTNSDKKGAAEFSPLSVS
                     VGKTTSEQEALEKTGATSSRTTDKNLQSLDATVRSMPGTYTQIDPGQGAISVNIRGMS
                     GFGRVNTMVDGITQSFYGTSTSGTTAHGSTNNMAGVLIDPNLLVAVDVTRGDSSGSEG
                     INALAGSANMRTIGVDDVIFNGNTYGLRSRFSVGSNGLGRSGMIALGGKSDAFTDTGS
                     IGVMAAVSGSSVYSNFSNGSGINSKEFGYDKYMKQNPKSQLYKMDIRPDEFNSFELSA
                     RTYENKFTRRDITSDDYYIKYHYTPFSELIDFNVTASTSRGNQKYRDGSLYTFYKTSA
                     QNRSDALDINNTSRFTVADNELEFMLGSKLMRTRYDRTIHSAAGDPKANQESIENNPF
                     APSGQQDISALYTGLKVTRGIWEADFNLNYTRNRITGYKPACDSRVICVPQGSYDIDD
                     KEGGFNPSVQLSAQVTPWLQPFIGYSKSMRAPNIQEMFFSNSGGASMNPFLKPERAET
                     WQAGFNIDTRDLLVEQDALRFKALAYRSRIQNYIYSESYLVCSGGRKCSMAEVIGNDW
                     EGISDEYSDNMYIYVNSASDVIAKGFELEMDYDAGFAFGRLSFSQQQTDQPTSIASTY
                     FGAGDMTELPRKYMTLDTGVRFFDNALTLGTIIKYTGKARRLSPDFEQDEHTGAIIKQ
                     DLPQIPTIIDLYGTYEYNRNLTLKLSVQNLMNRDYSEALNKLNMMPGLGDESHPANSA
                     RGRTWIFGGDIRF"
     gene            4546..5721
                     /gene="senB"
                     /locus_tag="OQQ48_003228"
     CDS             4546..5721
                     /gene="senB"
                     /locus_tag="OQQ48_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterotoxin production-related protein TieB"
                     /protein_id="HCS1953904.1"
                     /translation="MNIFTLSKAPLYLLISLFLPTMAMAIDPPERELSRFALKTNYLQ
                     SPDEGVYELAFDNASKKVFAAVTDRVNREANKGYLYSFNSDSLKVENKYTMPYRAFSL
                     AINQDKHQLYIGHTQSASLRISMFDTPTGKLVRTSDRLSFKAANAADSRFEHLRHMVY
                     SQDSDTLFVSYSNMLKTAEGMKPLHKLLMLDGTTLALKGEVKDAYKGTAYGLTMDEKT
                     QKIYVGGRDYINEIDAKNQTLLRTIPLKDPRPQITSVQNLAVDSASDRAFVVVFDHDD
                     RSGTKDGLYIFDLRDGKQLGYVHTGAGANAVKYNPKYNELYVTNFTSGTISVVDATKY
                     SITREFNMPVYPNQMVLSDDMDTLYIGIKEGFNRDWDPDVFVEGAKERILSIDLKKS"
     gene            6180..6530
                     /locus_tag="OQQ48_003229"
     CDS             6180..6530
                     /locus_tag="OQQ48_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCS1953905.1"
                     /translation="MPSGRHTARIRTGHDSGTVSPFVHSTLSPLVPVYPVFITVIADH
                     DKPQPSGCLLESQGSLCPICRQRITHETGWNVHHKVKKVMGGGEELSNLVLLHPNCHR
                     QLHSCEAGSLKKGL"
     gene            6673..6894
                     /locus_tag="OQQ48_003230"
                     /pseudo
     CDS             6673..6894
                     /locus_tag="OQQ48_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(6915..6977)
                     /locus_tag="OQQ48_003231"
                     /pseudo
     CDS             complement(6915..6977)
                     /locus_tag="OQQ48_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            7428..8840
                     /gene="ospC1"
                     /locus_tag="OQQ48_003232"
     CDS             7428..8840
                     /gene="ospC1"
                     /locus_tag="OQQ48_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC1"
                     /protein_id="HCS1953906.1"
                     /translation="MNISETLNSANTQCNIDSMDNRLHTLFPKVTSVRNAAQQTMPDE
                     KNLKDSANIIKDFFRKTIAAQSYSRMFSQGSNFKSLNIAIDAPSDAKASFKAIEHLDR
                     LSKHYISEIREKLHPLSAEELNLLSLIINSDLIFRHQSNSDLSDKILNIKSFNKIQSE
                     GICTKRNTYADDIKKIANHDFVFFGVEISSHQKKHPLNTKHHTVDFGANAYIIDHDSP
                     YGYMTLTDHFDNAIPPVFYHEHQSFLDKFSEVNKEVSRYVHGSKGIIDVPIFNTKDMK
                     LGLGLYLIDFIRKSEDQSFKEFCYGKNLAPVDLDRIINFVFQPEYHIPRMVSTENFKK
                     VKIREISLEEAVTASNYEEINKQVTNKKIALQTLFLSITNQKEDVALYILSNFEITRQ
                     DVISIKHELYDIEYLLSAHNSSCKVLEYFINKGLVDVNTKFKKTNSGDCMLDNAIKYE
                     NAEMIKLLLKYGATSDNKYI"
     gene            complement(8944..9092)
                     /locus_tag="OQQ48_003233"
                     /pseudo
     CDS             complement(8944..9092)
                     /locus_tag="OQQ48_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            9168..10865
                     /gene="ospD3"
                     /locus_tag="OQQ48_003234"
     CDS             9168..10865
                     /gene="ospD3"
                     /locus_tag="OQQ48_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HCS1953907.1"
                     /translation="MPSVNLIPSRKICLQNMINKDNVSVETIQSLLHSKQLPYFSDKR
                     SFLLNLNCQVTDHSGKLIVCRHLASYWIAQFNKSSGHVDYHHFAFPDEIKNYVSVSEE
                     EKAINVPAIIYFVENGSWGDIIFYIFNEMIFHSEKSRALEISTSNHNMALGLKIKETK
                     NGGDFVIQLYDPNHTATHLRAEFNKFNLAKIKKLTVDNFLDEKHQKCYGLISDGMSIF
                     VDRHTPTSMSSIIRWPNNLLHPKVIYHAMRMGLTELIQKVTRVVQLSDLSDNTLELLL
                     AAKNDDGLSGLLLALQNGHSDTILAYGELLETSGLNLDKTVELLTAEGMGGRISGLSQ
                     ALQNGHAETIKTYGRLLKKRAINIEYNKLKNLLTAYYYDEVHRQIPGLMFALQNGHAD
                     AIRAYGELILSPPLLNSEDIVNLLASRRYDNVPGLLLALNNGQADAILAYGDILNEAK
                     LNLDKKAELLEAKDSNGLSGLFVALHNGCVETIIAYGKILHTADLTPHQASKLLAAEG
                     PNGVSGLIIAFQNRNFEAIKTYMGIIKNENITPEEIAEHLDKKNGSDFLEIMKNIKS"
     gene            10982..11101
                     /locus_tag="OQQ48_003235"
                     /pseudo
     CDS             10982..11101
                     /locus_tag="OQQ48_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
BASE COUNT         3483 a         2424 c         2420 g         3003 t
ORIGIN      
        1 ccctccgttg taatgtgcca gccgggaggc cgctatcggc ggcttcttca gataacgacc
       61 caggtagcgc gccgtattcc tcccgccggc cattttcttc gacatgtaca tcaggcgcgg
      121 aactcatcgg ggcgcgggaa tgaaatccca ttcagactcc ggatagattc aagcaagccc
      181 acttatcgtc aaaatcggtg ttgcaaaaac gggagtgacc atagatccgt tttccaagcg
      241 gaccccataa tatcggctgg taaacatcac tcgctagtgc attctttttg ttggtaatga
      301 taattattta catttattct ttgatgtttt atcataatta atgttttatc aataaatata
      361 tggaaatata ttgtttcaga tgggaatatg agaaagttca tccttatttc tatgataacg
      421 ttagctagtg ttagtctggg agcctgcaga cagaatgcca ccataaagca ggatgcgcca
      481 gggcagaaag ctttcctgat tgacgggcag atttacgatt tgcacagtgg caaaattatt
      541 tctagcagcg agcttttggc ggatcttgct accgctcgac atcttattat tggtgaaaaa
      601 cacgataatg ccgagcatca tcagattgag ctttggctta ttcaaaatct gctcatccag
      661 cgaccacagg ggagtgtgtt gcttgagatg ttgacgtcag accaacagcc ccgagttaac
      721 caagttaaat gttggctgaa agataatcct gtggttcgag atagccgtgt tcaggaacta
      781 cttaactggc aaaaagggtg gtcctgggag atgtatggcg acatcgtcat gcagctatta
      841 cgtgggcctt acccgctgct caatgccaac attggccggg agcagatgtt ggcactgtac
      901 aaaaaaaacg aatttcctaa aggaaaaaaa tccactgcac ctgtggtaca agaagctctg
      961 cgtgaaacca tcatagctat gcatgaaggc aaccttgaaa gccagcaact gacgtcaatg
     1021 ctctctgttc agcagcagcg ggatcgctac atggcccgcc agcttctgag tgccccggta
     1081 ccgtcgttgt tgattgcagg tggttatcac gcgtcaaaaa acatgggcgt accgttacac
     1141 atggaagatc ttgccaccgg cacccatcca gtggtgttga tgcttgcaga aaaaggaatg
     1201 aacatcacag tagatcatgc tgactacgtg tggtttgtag cgccagatac aacaaaaagg
     1261 taaaccatgg atgatgaata ttctccactt cccacagtcg gtaaaatgga gcagctggtt
     1321 tatctgttct ctactgcttc atggactgat tttccttacg tttatctggc gtttttccga
     1381 agttcagccc gctatgtctc ctgcaccggc gatcatgctg caatgggcag aaattataga
     1441 ggctccttct tctccacttt cgcttccggt gggaatcgct caacaggagt ccgcagtgac
     1501 agaagaaaag caacaaactg aggacagaca acaacgtcct gtcactgaag acagtgatgc
     1561 aaccattgaa ataactagga agaaaaagtc atccgatgga gaaaagaaaa agacacgtcc
     1621 acctcgtaag ataaaggctc aaaccagcga cagcaacccc acagccgttt ccagtaatgc
     1681 tgcacctcag gctctggtcg aatcgtcccg tattgctgcg ccttttaaca gtgactcaac
     1741 aaagcgagat aattctgagg catcttggga aagcagagta aaaggccacc ttaaccgtta
     1801 taaacgttac cctggcgatg ccagaaaacg tgcacgaacc ggcacagccg ttgtgacttt
     1861 taccgtaaac acggaaggca ctattgtcag tagtttcttg gaaatctcgt caggaaccct
     1921 ttctcttgac cgcgaagcta ttgctgtttt agaacgcgct caaccacttc ccaaaccacc
     1981 gcctgaaata ttggagggag gattatttaa agtaaaaatg ccgattacct ttaagttgaa
     2041 ggagtaaagg gataattggc cgttcaagtt catttaactg aaatttaatt ttcattttta
     2101 tgctgaacat tttatcaaac ttttccactg tcgaaaaaat caaaaagaca gtagatcata
     2161 attaataaac aaatagttat acttatcttt attaatatta tatccaagga aatttatgaa
     2221 cgttataaaa ctggctatcg gctcaggcat attattgctc agctgcggtg cttactcaca
     2281 atccatcagt gaaaaaacta attccgacaa aaaaggagcg gcagaattca gtccgctcag
     2341 cgtttctgtc gggaagacga ccagtgagca ggaagctctc gagaaaacag gcgcgaccag
     2401 ttcccggaca acggacaaaa acctgcaatc acttgacgca acagtgcgta gtatgcctgg
     2461 tacttatact caaatagatc ctggtcaggg agcaatcagt gtgaatattc gaggcatgag
     2521 cggatttggt cgtgtaaaca ctatggtcga tggtattacc cagagttttt acggaacctc
     2581 tacctccgga acaacggcgc atggttcaac taacaatatg gctggcgtac ttatagatcc
     2641 taacttactg gtagcagttg atgttacacg cggtgacagc agtggctctg aagggatcaa
     2701 cgcccttgcc ggtagtgcaa atatgcgtac tattggcgtt gacgatgtaa tatttaacgg
     2761 taatacatat ggccttcgtt cacgtttctc tgtcggtagt aatgggctgg gacgcagcgg
     2821 aatgatcgcc cttggtggaa aaagcgacgc ttttacggat acgggaagca ttggcgttat
     2881 ggctgctgtg agtggcagtt ctgtgtactc taatttctca aatggttctg gaattaacag
     2941 caaagagttt ggttatgata aatatatgaa gcagaacccc aaatcccaac tgtataaaat
     3001 ggatatcaga ccagacgaat ttaacagctt cgaactttcc gctcgaacct atgaaaataa
     3061 atttacacgt cgtgatataa ccagtgacga ctattacatt aaatatcatt acaccccttt
     3121 ttctgaatta attgacttta acgtaacggc cagtaccagt cgcggtaatc aaaagtatcg
     3181 tgatggctcg ctgtatactt tctacaaaac ctcagcgcaa aatcgttctg acgcgctgga
     3241 tatcaacaat accagccggt tcactgtcgc ggacaatgaa ctggagttta tgctgggcag
     3301 caaactgatg cgtacccgct atgaccggac cattcactca gcggcgggcg acccgaaagc
     3361 gaatcaggaa tcgatcgaga acaatccgtt cgcaccctcc ggccagcagg atatttcagc
     3421 gctgtatacc gggctgaagg ttacgcgcgg catctgggag gcagatttca atctcaacta
     3481 cacacgtaac aggatcacag ggtacaagcc cgcctgcgat tcacgcgtta tctgcgtgcc
     3541 acagggtagc tacgatattg acgataaaga gggtggcttc aacccttcag ttcagctttc
     3601 tgctcaggta acaccatggc ttcagccgtt cattggctac agcaaatcca tgcgcgcccc
     3661 gaacatccag gagatgttct tctctaattc aggaggcgca tccatgaacc cattcctgaa
     3721 gcctgaacgt gcagaaacct ggcaggcggg ttttaacatt gataccagag atttactggt
     3781 cgaacaggat gccctgcgct ttaaggctct ggcgtaccgc agcaggatcc agaactacat
     3841 ctacagcgag tcttatctgg tttgttctgg aggtcgtaaa tgcagtatgg ctgaggtgat
     3901 tggcaatgac tgggagggca ttagcgatga atacagcgac aatatgtaca tctacgttaa
     3961 ctcggcaagc gacgttattg caaagggctt cgaactggag atggattatg atgcaggttt
     4021 tgcttttggc cgactctctt tcagccagca gcaaacagac cagccaacct ccatcgccag
     4081 cacctacttt ggcgcagggg atatgaccga actgcccaga aaatacatga cgctggatac
     4141 tggtgttcgc ttcttcgata acgcgttgac cctgggcact atcataaaat acacaggcaa
     4201 ggctcgtcgc ctgtcgcctg attttgagca ggacgaacat accggcgcaa taatcaaaca
     4261 ggatttgccg cagatcccaa cgattatcga tctctatggt acttacgagt acaaccgcaa
     4321 cctgacactg aaactttcgg tacaaaacct gatgaacaga gattattcgg aggcgctgaa
     4381 taagctcaac atgatgccag gtcttggtga cgagagccac ccagccaatt ccgcgcgtgg
     4441 cagaacatgg atatttggcg gggacattcg tttctgatga caatttccgc caggttcaga
     4501 caatacgttt tctctcttat gtcaatttta ttgcaggaac gaaaaatgaa tattttcact
     4561 ttatccaaag caccgctata cttgttaatt tcactatttt tacccacgat ggccatggct
     4621 atcgatccac ctgaacgcga actttcgcga tttgccctga aaacgaatta ccttcagtcc
     4681 cctgatgaag gcgtctatga actggcgttt gataatgcca gtaaaaaggt gtttgcagca
     4741 gtcaccgatc gtgtaaatcg tgaagccaat aaaggctatc tgtattcgtt taattcagat
     4801 tcgctgaaag tcgaaaataa atacacgatg ccataccggg cattttcgct ggcgataaat
     4861 caggataaac atcagctcta tatcggacac acccagtcag cgtccctgcg tatcagtatg
     4921 tttgacaccc caaccggtaa actggtaaga accagcgaca ggttaagttt taaagcggca
     4981 aacgctgcag attcgcgttt tgagcatttg cgccatatgg tttacagcca ggattccgat
     5041 accctgtttg tgagttatag caatatgctg aaaacggccg agggcatgaa gcctctgcat
     5101 aagctgttaa tgctcgacgg gacgacgctt gccttaaaag gcgaggttaa ggatgcttac
     5161 aaaggtacag cgtatggtct gacgatggat gaaaaaacac agaaaatcta cgttggcgga
     5221 agagattaca tcaacgaaat tgatgcgaaa aatcagacgc tgctgcgtac catcccgttg
     5281 aaagatccga gaccacaaat cacaagtgtg cagaatctgg cggtggactc cgcttctgac
     5341 cgtgcctttg tggtggtatt cgaccatgac gatcgttccg gtacaaaaga tggcctctat
     5401 atttttgact tacgcgacgg taaacagctt ggctatgtgc acacaggagc cggagctaac
     5461 gcggtgaaat acaatccgaa atataacgaa ctgtatgtca ccaacttcac tagcggcacc
     5521 atcagcgtag tggatgccac caaatacagc atcacccgtg aatttaacat gccggtctac
     5581 ccaaaccaga tggtgttgtc ggacgatatg gatacccttt acattggcat caaagaaggc
     5641 tttaaccgcg attgggatcc tgatgtgttt gtggaaggag ctaaagaacg tattctgagc
     5701 attgatttga aaaagtcgtg atcgggggaa aaaacatggg ataaaaactc cgttattcca
     5761 ctaaactggc cggactagga aaagcaaaca tctctgtcct gccggtacgc ctgttacgcc
     5821 gctccatgag gctgaggaat ctccagaaaa cgagacaggc acagcattgt acgatcaact
     5881 gatttgtgcc aataattcaa tgaaatcaca gctatgcctg ttcacgtata ggaaattgaa
     5941 aaactaagcg ataccactgt ccgcgtctta tgcctcgtgc ggtaagctgt ttttcacatc
     6001 acgccggtgt ttttttcttt ctgtggacgg ttttaatact gtaatcggcc gttttttata
     6061 cttaccccga ttcccgggcg cacttatcct gcatcacccc gggatatacc tcagcagact
     6121 gatatcccgc gcgtttttct tcagcccctc cacattcagt cccgcgatgg cccggcggta
     6181 taccatccgg ccgccacaca gcacgcattc gaacgggtca cgactcagga actgtttcac
     6241 catttgtgca tagcacactt tcgccactgg ttccggttta tcctgttttt ataacggtaa
     6301 ttgcagacca tgacaaaccg cagccgtcag gctgcctact cgagtcacaa ggctctctgt
     6361 gtccgatatg ccggcagcgc attacccatg aaactggctg gaacgtacac cataaggtga
     6421 agaaagttat ggggggcggt gaagaattat ctaaccttgt cttgttacat ccaaattgcc
     6481 atcgacagtt acacagttgt gaagccggct ctctcaaaaa gggcttataa aggcttgagc
     6541 ggtgtaccgg gaaaccggta tgcaccgttc ttaggggagg gcagtccagc aatgaactgt
     6601 ccttacccga caaacatcca aaacgaacgg aacgtgctgg aatccgcata taacccgttg
     6661 taaagtgagg aaatggacac ctcacttgct catgagaacg cccgcctgcg ggcactgttg
     6721 cagacgcaac aggacactat ccaccagatg gctgaataca accgcctgct ctcacagcgg
     6781 atggcggctt atgcttccga aatcaaccga ctgaaggcgc tggttgcgaa actgcagcgt
     6841 atgcagttcg gtaaaagccc agaaaaactt cgcgcaaaaa ccgaacggca gattccattt
     6901 tcacgggcga tctacgccac acaggctctg ggttgcagtg actgctccat taaggcgatt
     6961 ttgaactcat aagggaagta acaaaaaaac tgcaaaagta caataattat atatggccaa
     7021 actaacctct attaaaaaaa catcagtttt tcacacttag ttgagcttca tgaaaaaatc
     7081 atcgaggatt tcattgcaga atacacattc cactaactgg aagcactcca taagaggaat
     7141 cagagaaagt atcgtttttt tatagtaaaa ttcattgctg ttcaataaaa ttgatataat
     7201 tattaaatta tggaaataat ttcacaaaaa agattaactt gagttcttaa gcatcggatt
     7261 ttagatattt tgaaatatct ggcatcacat aacatcaaaa gtatataata cccttatcta
     7321 attacatgca gtatattgta tatatactca aaaattgtaa ttcataccct actatatctt
     7381 gtggggaaag atattaatta aaactgtttt catataaggt tcattttatg aatatatcag
     7441 aaacactgaa ctcagcaaat acccaatgca atatagattc tatggataac agattacata
     7501 cattgtttcc aaaagtgaca tcagtgcgaa acgctgcaca acaaactatg ccagatgaaa
     7561 aaaatttaaa agatagtgca aatattatta aagatttctt taggaaaact atagcagcac
     7621 aaagttatag tagaatgttc tctcaaggct ctaactttaa atctttaaat atagcaattg
     7681 atgcaccatc agacgctaaa gcctcattta aggctattga gcaccttgac agattatcga
     7741 agcattatat atctgaaata agggaaaaac ttcatcctct ttctgcagag gaactcaatt
     7801 tgctttcgct aattattaat tctgatttaa tcttcagaca tcaaagtaat tctgatttgt
     7861 ctgataaaat tttaaacatt aagtcattca ataaaattca gtctgaagga atatgcacaa
     7921 aacgaaacac atacgctgat gatataaaaa aaatagctaa tcatgacttt gtgttttttg
     7981 gcgttgaaat ctctagccat cagaaaaaac accccctgaa tacaaaacat cacactgttg
     8041 attttggtgc aaatgcgtat atcattgatc atgactctcc atatggatat atgacattaa
     8101 ccgatcactt tgataatgct attccacctg ttttttacca tgagcaccaa tcatttttag
     8161 ataaattttc agaggttaat aaagaagtta gtcgatacgt acatggaagt aaaggaatta
     8221 tagatgtacc aatattcaat actaaagata tgaagttagg gctcggatta tacctgattg
     8281 actttattag aaaaagtgaa gaccaaagct tcaaggagtt ttgctatgga aaaaatcttg
     8341 cccctgtgga tctggataga atcataaact ttgtttttca gccagagtac catataccta
     8401 ggatggtaag tacagaaaac ttcaaaaaag ttaagattag agaaatatcc ttagaggagg
     8461 ctgttacagc atctaattac gaagaaatta acaagcaggt cactaacaaa aaaattgctc
     8521 tccagactct ttttctttcg attactaatc aaaaagagga tgtcgcctta tatatattat
     8581 ctaattttga gataactaga caagatgtta tttccataaa gcatgagttg tatgatattg
     8641 agtatctact tagcgctcat aattcaagct gtaaagtact tgagtatttt atcaataagg
     8701 ggttggttga tgtaaacaca aagttcaaaa aaactaatag tggggattgt atgttggata
     8761 acgcaataaa atatgagaat gcagaaatga taaaactatt attgaaatat ggtgcaacat
     8821 ctgacaataa atatatttaa tcaaaattga atatcgttta gcaaaaggca gtatttgcac
     8881 aaaatatcga tatattgttt attgtcagta tggctcaatg tgataatagt tggaaagttt
     8941 gatgggtttc gccccgttgt agcggtagtc gagctggaag gtcttcaggc actgcttaca
     9001 gcgatagagc agcccccctg aactggaatg gccgttccgg ataccccctg agtttcagag
     9061 taacggggac aaaccacatc aatctttgcc atcaatcatc caaagggcaa agagtacaac
     9121 aacactaagt ctgcgtcaca acccatcaat gaaaggaata tatacatatg ccatcagtaa
     9181 atttaatccc atcaaggaaa atatgtttgc aaaatatgat aaataaagac aacgtctctg
     9241 ttgagacaat ccagtctcta ttgcactcaa aacaattgcc atatttttct gacaagagga
     9301 gttttttatt aaatctaaat tgccaagtta ccgatcactc tggaaaactt attgtctgtc
     9361 gacacttagc ttcctactgg atagcacagt ttaacaaaag tagtggtcac gtggattatc
     9421 atcactttgc ttttccggat gaaattaaaa attatgtttc agtgagtgaa gaagaaaagg
     9481 ctattaatgt gcctgctatt atttattttg ttgaaaacgg ttcatgggga gatattattt
     9541 tttatatttt caatgaaatg atttttcatt ccgaaaaaag cagagcacta gaaataagta
     9601 catcaaatca caatatggca ttaggcttga agattaaaga aactaaaaat gggggggatt
     9661 ttgtcattca gctttatgat cccaaccata cagcaactca tttacgagca gagtttaaca
     9721 aatttaactt agctaaaata aaaaaactga ctgtagataa ttttcttgat gaaaaacatc
     9781 agaaatgtta tggtcttata tccgacggta tgtctatatt tgtggacaga catactccaa
     9841 caagcatgtc ctccataatc agatggccta ataatttact tcaccccaaa gttatttatc
     9901 acgcgatgcg tatgggattg actgagctaa tccaaaaagt aacaagagtc gtacaactat
     9961 ctgacctttc agacaatacg ttagaattac ttttggcagc caaaaatgac gatggtttgt
    10021 caggattgct tttagcttta caaaatgggc attcagatac aatcttagca tacggagaac
    10081 tcctggaaac ttctggactt aaccttgata aaacggtaga actactaact gcggaaggaa
    10141 tgggaggacg aatatcgggt ttatcccaag cacttcaaaa tgggcatgca gaaactatca
    10201 aaacatacgg aaggcttctc aagaagagag caataaatat cgaatacaat aagctgaaaa
    10261 atttgctgac cgcttattat tatgatgaag tacacagaca gatacccgga ctaatgtttg
    10321 ctcttcaaaa tggacatgca gatgctatac gcgcatacgg tgagctcatt cttagccccc
    10381 ctctcctcaa ctcagaggat attgtaaatt tgctggcctc aaggagatat gacaatgttc
    10441 ccggacttct gttagcattg aataatggac aggctgatgc aatcttagct tatggtgata
    10501 tcttgaatga ggcaaaactt aacttggata aaaaagcaga gctgttagaa gcgaaagatt
    10561 ctaatggttt atctggattg tttgtagcct tgcataatgg atgtgtagaa acaattattg
    10621 cttatgggaa aatacttcac actgcagacc ttactccaca tcaggcatca aaattactgg
    10681 cggcagaagg cccaaatggg gtatctggat taattatagc ttttcaaaat aggaattttg
    10741 aggcaataaa aacttatatg ggaataataa aaaatgaaaa tattacacct gaagaaatag
    10801 cagaacactt ggacaaaaaa aatggaagtg attttctaga aattatgaag aatataaaaa
    10861 gctgaatatt atctcgtggt gatacactga atttgaggta accggctcat ttaaaccgtc
    10921 tggtctgtta cctccggttc cacaaaaata atgtccatca ttttgaagtg gacactatcg
    10981 tatggaatac cggacctgga tcactgaagc tttacgcctt catttcgaag aacatttacc
    11041 acgggttgtg gccggacgtc gcctgggcgt accaaaatca actgtctgtg gtatgttcgt
    11101 gtgaaccgcc cccggtatcc cgctcatgct ggcgaaccca gacacgcagt gaaccgcccc
    11161 gggtttcctg gagagtgttt tatctgtgaa ccacggcagc ctggcggctg caaatcgggg
    11221 tgtggcagaa tatgagcttt cagagtaacc atccagaatg agttcccatg tcacacaacc
    11281 tggagcacca gaaagtccac accagaatgg tgaaagaggt tctgaaagcc
//
LOCUS       DAJVUJ010000136        11301 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.136, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000136 DAJVUJ010000000
VERSION     DAJVUJ010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11301
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.136"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..816
                     /locus_tag="OQQ48_003236"
     CDS             <1..816
                     /locus_tag="OQQ48_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HCS1953908.1"
                     /translation="DSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTSAEKS
                     QAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAKALHD
                     SFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLEIPEL
                     SGKLKGHAQRVPVKTGSVTELVSILGKKATAEEVNNALKQATTNNESFGYTDEEIVSS
                     DIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            complement(858..2298)
                     /gene="aldA"
                     /locus_tag="OQQ48_003237"
                     /pseudo
     CDS             complement(858..2298)
                     /gene="aldA"
                     /locus_tag="OQQ48_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
     gene            complement(2453..3295)
                     /locus_tag="OQQ48_003238"
     CDS             complement(2453..3295)
                     /locus_tag="OQQ48_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCS1953909.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALFNQAVERVHTAIVVQDPTMQRSTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLRQKLPVCEPLV
                     CKRA"
     gene            complement(3567..7469)
                     /gene="hrpA"
                     /locus_tag="OQQ48_003239"
     CDS             complement(3567..7469)
                     /gene="hrpA"
                     /locus_tag="OQQ48_003239"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HCS1953910.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSGTIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVLGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            7670..8275
                     /gene="azoR"
                     /locus_tag="OQQ48_003240"
     CDS             7670..8275
                     /gene="azoR"
                     /locus_tag="OQQ48_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCS1953911.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(8326..9642)
                     /locus_tag="OQQ48_003241"
     CDS             complement(8326..9642)
                     /locus_tag="OQQ48_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HCS1953912.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSTTFEQRRLVHRLILATVMACCG
                     FLLYPLKFSFIRPEVSGVTGWPFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAER
                     WRKVCGGWFLLISISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGAGSCIVLMELMVMIQLWWPVWLCWPVLSLLIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKITAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGHCKTVDEAISYIRARRSHIVLKEDHKAMLKLWENR"
     gene            complement(9632..11301)
                     /locus_tag="OQQ48_003242"
                     /pseudo
     CDS             complement(9632..11301)
                     /locus_tag="OQQ48_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415928.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
BASE COUNT         2818 a         3067 c         2693 g         2723 t
ORIGIN      
        1 gattcactta tcgttgatgg gaaaagtatc gcggtttacg ccgaaaaaga ggctaaaaat
       61 attccgtgga aagcgaaagg tgcagaaatc attgtcgaat gtactggctt ttatacctcc
      121 gccgagaaat cgcaggcgca tcttgatgct ggtgcgaaga aggtgttgat ttccgcccct
      181 gccggtgaaa tgaaaactat cgtttataac gtcaatgacg acactctgga tggcaacgac
      241 accattgttt ccgtggcgtc atgcaccact aactgtcttg cgccgatggc caaagccttg
      301 catgacagtt tcgggataga agtcggcacg atgacgacca ttcatgccta tactggcacc
      361 cagtcactgg tggatggccc acgtggtaaa gatttacgtg cttcacgcgc agcggcagaa
      421 aatatcattc cccacactac gggggcggca aaagccattg gtctggagat cccggaactg
      481 agcggcaaac tgaaaggtca tgcgcaacgc gtgccggtga aaacaggttc ggtcactgaa
      541 ctggtatcga ttctcggaaa aaaagcgact gccgaagagg tgaataacgc acttaaacaa
      601 gcaaccacca ataacgagtc atttggttat accgatgaag aaatagtctc ttccgatatc
      661 attggcagcc atttcggttc ggtgtttgat gccacgcaaa cggaaattac cgccgtgggc
      721 gatttacaac tggtgaaaac ggtcgcctgg tacgataacg aatatggctt cgtcacgcag
      781 cttattcgca ccctcgaaaa attcgctaaa ctctgacgcg cacaggcgga ggaaaaaacc
      841 tccgcctctt ttactcatta cgactgtaaa taaaccacct gggtctgcag atattcatgc
      901 aggccatgtt taccatctgc gccgccaata ccggatttac gccatccggc gtggaagcct
      961 tgcatagctt cgaagttttc acggttgatg taagtttcac caaacttcag ccctttaatg
     1021 gctttcatcg cgacgttcag attttgggta tagatttgat gaggtcaggc cgtaatcact
     1081 gtcattagcc attgagatag cctcttccag cgtgtcaaat gcgaccaccg gcagaaccgg
     1141 gccaaaggtt tcctcatgca taatcgacat ttcctggcga acatccagca gcaatgtcgg
     1201 cggataataa tatcctttcc cctctaccgc tttgccaccc aacgccactc tcgccccttc
     1261 ttctactgcg cgcgccactt tttgctcaac cctttccaac gccgcggcgt taatcaacgg
     1321 ccccatcgca atgtcgttgc gttcagcggg gttaccaaat tgaaccgcct gcatcgcttc
     1381 acccagccga ttgacgaact gatcataaat gcctttctgt acataaacac gttctgcaca
     1441 gttacacact tgcccactat taatgacgcg tgaatcaacg atggctttga ctgccagttc
     1501 aagatcggca tcgtccatta cgatagctgg tgctttaccc cccaattcca gacacacttt
     1561 ggtgatgttt ttcgccgcag tcgccatgat cttctcacct gcagagacgc tgcctgtcat
     1621 actgaccatt gcgacctttg ggttacccgc cagttcttgc ccaacggttt caccacgccc
     1681 cagtacaagg ttaaacacgc cgcgcggaag gcctatttca tcgacgattt tggcgaatgc
     1741 aatcgcattg tttggcgtaa attcactagg tttaatgacg atggtattac cggtcaaaag
     1801 agcgggagcc attttgcggg caatgaggaa gaacgggaag ttccacggca gaatgccggt
     1861 agtcacacca agcgcacgtt taaacaaaag aatattttct cctggacgat cgctttgaat
     1921 aatctcgccc tcgtaacgcc gtgcccactc cgccatgtaa tcgatatagt cggcagtaaa
     1981 agccacttcg acttcagcca gctgctggat cttgccccct tcttcaacaa tcagcgcact
     2041 gatttcactg gcgcgttcgc ggatcccggc ggagattttg cgcaaccaac tggcgcgttc
     2101 aatagcaggc aacgcttccc attctggttg tgcacgttct gctgcatcga ttgccttacg
     2161 ggcatcctcg gcctgaccat cgggtatgcg ggaaatgaca gcctctgtag cagggtttac
     2221 cacatcaatc catgcgtctc cacgccaggt aacaaactgt ccatcgatat acataggatg
     2281 ttgaacgggt actgacatgg gcgactcctg tgatttatat gtttgttctt ggtaaataca
     2341 ttgttaatta acatcaatca tcatagagac ggcatcgtga aaggacaatt ctgtgaaggg
     2401 cttcataaaa acaagtgtaa aaggaataaa aggcagaaat aaaagtcagg agtcatgccc
     2461 gtttgcaaac aagtggttca caaacgggca atttttgacg taacgagcga ctttccatcg
     2521 cctcgatgag caccgcatcg tgtttaagcg tttgccatgc atggatgact tctgccggaa
     2581 aatcaacgtg aacgataaaa tctcgcccgc ggggaccgta gccatcgcta tcatcgcgta
     2641 aacgcggcag ctcgccagtg agtagtgaga gataacgctc aaccggccat aatccttgta
     2701 actgattaat aaagattaca ctgtctgcgt tatttcctaa ctgaggaacg aatccgggat
     2761 aacttaacca gcgtggtgca tcgggattgt ccccagtcat acggcggaac gtcgccatcg
     2821 tgctccgctg catggtgggg tcctgaacaa cgatagccgt atgaactcgt tctacggcct
     2881 gattaaatag cgcgatgcta aagcgtgcgt tttcaccgca gtttgttgac tggtcttcaa
     2941 tccagatttt ttcatgcgga atgtgccaga actgatgagc gatatccgcc aggatggtcg
     3001 cttctgctct gccagtggtg cggatagtgt tgtagtgcgg atgctgtgcg atggcgctat
     3061 acaaaaaagt tgtcgagtga ccgataccac cactaatcag taaaggaatt tgctgatcgc
     3121 gagcaatctt acatgccgcg tcgatagtcg gcataaccgc attgcctgca aggatcacgc
     3181 aatcggcctg atacggcacc tcaccggaga aatcatcctg cgccagccac tgtccgataa
     3241 catttatggc atctatagtt gccggcgaaa gcgtcggaaa cggggtaatg ttcatggttc
     3301 ctccttatcg ttcccttaag aatactgcgt gagataatag tgagaagctg agcactctga
     3361 aaggaggaag tcgtatgtcc ttaaccgcca ctccgcaagc ctgacatatc ttttaaggaa
     3421 atgctgtaga taaacattat tcatcttgca gcagagaatt gtatttttct gaattcctga
     3481 atttttaccc ttaccgtttt agaattaata aaaaacccgg cgctaaatgc ttaccgggtt
     3541 ttagatttat taggcaaata gcagggttaa ccgctaatct gctccatcgc ctgcaaaata
     3601 cgcttatctg aaatcggata aggcgtacca agttgttgag cgaagtaact aacgcgcagc
     3661 tcttctatca tccaacggat ctctttcacg tcttcatcct cacgacgtgc aggcggcagt
     3721 ttgttgatcc attgctgcca cgcctgctgg acgttttcga ctttcagcat ctgtgcacga
     3781 tcgcgatgtg gatcaaccgc cagtttttcc agtcgttttt caatcgcctg caaatatcgc
     3841 agcgtgtcgc ccagccgttt gaagccgtta ccagtgacaa aaccgcgata taccaacccg
     3901 cccatctgcg ctttaatgtc agaaagcccc agtgccatgg tcatatccac ccgccctttc
     3961 agacgtttgt tgatattgaa cactgccgta agaatttgct cgacctgctt cgcaatatct
     4021 accaccgtgt cgttcagttc ggcacgcact ttctcatgca gcgcagcaaa gccttcttcc
     4081 gtccagaccg ggccaccatt ggcgtcgatc agttgatcca caccgcagga gatacagtcg
     4141 tcgatcagct ccagcacttt gccatacggg ttgaagtaca gtcccagctt ggctttgttc
     4201 ggtaactttt catgtaaata cttgatcggc gagggaatat ttagcagcag tagacggcga
     4261 agaccattcc acattgcctg tttttgctcc agcgggttat caaacagttt gatcgccacg
     4321 ctgtcgcgtt catccaccag cgccggccac gccttcactt tatagttgcc acgcttctgt
     4381 tcgtaacttt ccggcagctg accaaaactc cagatatgta agccgctctg ctcgataccg
     4441 tcatccgcca ccgcggacag cgtttcctgc actttgcctt tcagcgcatc tttcagatct
     4501 tgtagcgagc gcccttcttt tagcttcttg tttttgtcat ccaccacgcg gaaggtgatt
     4561 ttcaggtgat cgggcacctg atcccagtgc cagtcttcgc ggtcaacggt aacgccggtc
     4621 atccgccgta actcgcgctc aaggctgtcg agcaacggta actccagcgg tgtgacgcgg
     4681 cctaaaaacg cttcggcgta gtttggtgca ggcacaaaat tacggcgtac cggtttcggc
     4741 aacgatttaa tcagagcaat caccaattcg cggcgcagac cggggatctg ccattcaaac
     4801 ccgctttcct caacctggtt aagtaacggc agcggaatat gtacggtcac accgtccgcg
     4861 tctgcgcctg gctcaaactg atagctcaaa cgcagcttga gattgccctg atgccagaag
     4921 ttcgggtaat ccagcttgct gattttttcc gccccttctt tgatcaacat gctcttttca
     4981 aagttgagca aatcaggcgt ttcgcggctg acttttttcc accagctatc gaagtgacga
     5041 gcggagatta catcgtggct gatgcgctgg tcgtagaact caaacagcgt ttcgtcatca
     5101 accagaatat cgcggcgacg tgatttgtgt tccagctctt ccacttcggc ccgcagtttc
     5161 aggttttcac ggaagaatgc gtgacgcgtc tgccagtcac cttccaccag cgcatggcga
     5221 ataaagagtt cacgacataa cgccggatcg atctggctgt agttgacctt gcgcgcggca
     5281 acaatcggca aaccataaac agtgactttt tccgttgcca tcaccgcgcc ctgcgcccgt
     5341 tcccagtgcg gttcgctgta ggtacgttta atcaaatgct gagcgacagg ctccacccat
     5401 tccgggtcga tacgcgcagc aatgcgcccc cacaggcggc tggtttctac cagttccgcc
     5461 accattaccc atttcggcgg ttttttgaat aaaccagaac cggggaagat ggagaaacgc
     5521 gcgttacgtg cgccggtata ttcttgttta tcggcatctt tcatgccgat atgggaaagt
     5581 aaacccgtca gcaacgcaat gtgaatttcg cgatactccg ccggttcgct gttaaccgga
     5641 atgccaagtt ctttcaccac ctgacgcaac tgggtgtaga tatcctgcca ttcgcgcacg
     5701 cgcagatagt tgagataatc ggtacgacac aggcgacgga aggcgtttga agaaagcgcc
     5761 ttttgctgct cgccaagata attccacaga tttacaaacg cgaggaagtc ggattctttg
     5821 tcgtggaagc gacgatgttt ttcgtccgat gcctgctgtt tgtccatcgg ccgctcacgc
     5881 gggtcctgaa tggagagcgc ggacgtgata atcatcgcct cacgcacgca gccatgtttc
     5941 tgcgcttcca gcaccatacg cgccagacga ggatcgaccg gcagttgcga gagctggcga
     6001 ccgagcggcg tcagtttata ggcgctggcc tgttcatccg tggtgatcgc gcccagttct
     6061 tcgagcagac gcacgccatc ctggatattg cgtttatccg gtgcttcgac aaacgggaac
     6121 gcagcgatat cgcccagccc cagcgcggtc atctgcaaaa taaccgaggc caggttggta
     6181 cgcagaatct ccggatcggt aaactccggg cgcgagagga aatcgtcttc ggaatagaga
     6241 cgaatacaga tcccttcgga cacacgacca caacggcctt tacgctgatt ggcagacgcc
     6301 tgtgaaatgg gttcaatcgg caaacgctgc actttggtgc gatagctgta gcggctgata
     6361 cgcgccgtac cggggtcgat aacgtattta atccccagta cagtcagcga ggtttccgcg
     6421 acgttggtcg ccagcacaat gcgtcgcccg ctgtgcgact ggaacacgcg gttctgttcg
     6481 ctattcgaaa gccgcgcata aagcggcaag atttcggtat ggcgtaagtt cagcttgttc
     6541 agcgcatcgg cggtatcgcg aatttcccgc tcgccgctca taaagatcag aatgtcgcca
     6601 gggctttcct ggctcagttc gtctacggcg tcaaaaatcg cctgcaactg atcgcgctcg
     6661 gtgtcatcgg cttcttcaac aatcgggcga tagcgcactt ccaccggata ggtccgaccg
     6721 gagacttcga taatcggcgc attattaaag tggcgagaaa agcgttccgg gtcgatagtc
     6781 ccggaagtga taatgatttt taggtcagga cgccgcggca gcaactcttt caaatagccg
     6841 agcagaaaat cgatattcag gctgcgttcg tgtgcttcgt caataatgat ggtgtcgtac
     6901 tgcatcagca ggcggtcttg ctggatctcc gccagcagga taccgtcggt catcagcttg
     6961 accatcgtgt tatcacttac gtgatcgctg aaacgcactt tgtaaccgat gcaaccgcct
     7021 ggctccgttt tcagctcttc cgcaatacgg ttcgccaccg ttcttgccgc cagacgacgc
     7081 ggctgggtat ggccgatcag tcctttaatc ccgcgcccca gctccataca gattttcggt
     7141 agctgggtcg ttttaccgga accggtttcc ccggcgacga tcaccacctg gtgatcacga
     7201 atcgcttcga gaatatcctg ttttttctga ctaactggca aattgtccgg ataagtgatt
     7261 tccggtcgtg ccgcttcacg cagcaggact ttccctgccg cctggtcaat ctctttcgcc
     7321 atctcctgga aaatggcctg ttgtgcatca ggatttttaa ccttcttaac accatgcaga
     7381 cggcgagaaa aacgcagtct gtcgcgcagc atcaacgaat ccagccgctg ctgcaaagca
     7441 gtaaaggtca atttttgttg ttctgtcata acgttagtgg gcagtagtct gcctgattaa
     7501 ttttctttta aatgatagat atagagtacc acatcgcggc tttctatgcg ttgttcaata
     7561 aattcgaaca taggcttcga tttattgcgc tatctctgtg gcaggattgt gaataaagtg
     7621 tcagcaagca acggggcatc gccaattcaa acatctataa ggaaacacca tgagcaaggt
     7681 attagttctt aaatccagca tcctggcagg gtactctcag tctaatcagt tgtccgatta
     7741 ttttgttgaa caatggcgcg aaaagcactc cgctgatgaa atcaccgttc gcgacctggc
     7801 tgcaaatccg attccggtac tggatggcga actggttggc gctctgcgtc cgagcgatgc
     7861 gccgctgact ccgcgtcagc aggaagctct ggcgctttcc gatgagctga ttgccgagct
     7921 gaaagcccac gacgttatcg ttattgcggc accgatgtat aacttcaaca tctcaactca
     7981 gttgaaaaac tattttgacc tggttgcacg cgcaggcgtt actttccgct ataccgagaa
     8041 cggtccggaa ggtctggtaa cgggtaaaaa agccatcgtt attaccagcc gcggtgggat
     8101 ccacaaagat ggaccaacgg acctagtgac gccgtatctg tccacgttcc tcggctttat
     8161 cggcattacc gatgtgaaat ttgtcttcgc cgaagggatc gcatacggtc cggaaatggc
     8221 agcgaaagcg cagtctgacg cgaaagcggc catcgacagc attgttgctg cataaccgga
     8281 cacgatcttg atgtggactg tagttcagtc cacatctcaa tccacttacc tgttttccca
     8341 taatttcagc atcgctttgt gatcttcctt aagcacaata tgcgaacgtc tggctcgaat
     8401 ataactaatc gcttcatcaa cggttttaca gtgtccgtaa cataacaacc atgccgccac
     8461 taccagcgca ctgcgcgata atcccaacgc gcaatggacc agaacgctgc cttgctcttc
     8521 gcgtaatgtt tccagcatcg ccacggcctg tcggagctcc ccctcttccg gaaccaccag
     8581 atccagcatc ggtacacaga aatagagtcg atcttttgtc gctcgtcccc gagggaattc
     8641 aaaggtgacg tccagaaccg cattctgtgc cggaatatgt cgtggaaacg cccctaaata
     8701 aacaccagca gtaattttgc tcaccggctc caggcgacga caaaaccaac gcatagacag
     8761 ccacatcccg atgcgccagg gcaatgtcag ccagtaaacg gcgggcggga gtttcccctg
     8821 actatctttc cctgttgtta tcgcgccaag cccaccgtac ccacggccaa taatgagtag
     8881 cgataatact ggccaacata accagactgg ccaccataac tgaatcatca ccattagctc
     8941 catcaacaca atgcacgagc ccgcgcctac gacatatggc agtgctattt tgattcgacg
     9001 ttgatcaggt ttctgataat tccaacgacg atcgacgggc accatccagt caatcaacat
     9061 acctaccgcc agccctgtga tgacatcaat aaaatgatgc tgccaggtcg tcagagtaga
     9121 aatagagatg agtaaaaacc atccgccaca gactttacgc cacctctcag ccagatgctg
     9181 acgaaagtga cgccaaagta gccagcagag aataatatgc agcgaaggag actggttata
     9241 aggcagatca aacagttcaa gttgcgaaaa tggccatccc gtcaccccac tcacttcagg
     9301 acggataaaa ctaaacttca gcggatagag caaaaaaccg cagcaggcca ttaccgttgc
     9361 cagaataagc cggtggacaa gtcggcgctg ttcgaatgtc gtgctacaaa cgaacagcga
     9421 aaatccatat aaaagatcca gactccagta aggaacaata gtccagggaa gaaaagggat
     9481 cgccgtttcc caaccgaata cctgactggg gatatcatgg ctgttaaggt cctgaaccgc
     9541 ggtgaactga ttaagagatc cataggtgaa gaaaaaaaac ggggccagca acaataacca
     9601 gccagcgcct tgtagcaata cgttccgacg ttcagttatc acgacgcacc gccatcgaaa
     9661 ccgtaaaaat accccactca tcaatccgtt gtgtgcattt atcaaatccg gcatcacgca
     9721 cgagtgaatc catctcccct tgcgaacgca cgcgcattac ccacggttta ccatctttat
     9781 gactggttaa caccccggca atcatctcca gttgagggtg ccacggctgc ccggtgtaga
     9841 tgagaatgcc gaccggttcg atggcattgg caagacctgc gagtgagttt tttacctgct
     9901 cattttcggg aaaaagctca tacagtccag agacaattgc cagcgtaggc cgcggagtta
     9961 acgcgctgag ttcctccggg ttaaacgcat cgccctgttc aaaacgcacc cgcccagaca
    10021 ttccccgttg ggcaatcatc tcttgcccct gtgcaacatt taactcgctg taatcacgta
    10081 acaaaatatc gcttacggca ggctcgtttg ccagcgcatc cagtacatag cgcccatgcc
    10141 ctgcggcaat gtcaaccacg cggacggcta aacctttggc gtggagatcg gcaacggctt
    10201 gtttaatcag tatttgtaaa tgggttttgc gctggcgaat accgcgccag ccaacactgt
    10261 tcaggtagat tttgtcgatt aagcgcccga atgcgttact accttgcggt tgattttgat
    10321 agacataatc cagcgaactg cctgaatcaa agccggtgct cattccgaga tgcaggcccg
    10381 cagagtgcgt cccgaacaat ttcatcgcct ttcgcataaa gcgatacgct aaatcaaccg
    10441 gcgataatag cacgggtcca ccagaaagta gccgccattg atccgctgat ggtcctgtgc
    10501 agtcttcatg ctgataatca aatttttgcg atttgttagc atataaacga ctaataaagc
    10561 tttgcatttt ttcaaatgcc agcgccctgt tctcttcacc caacgtgtcg tgataaaagc
    10621 ctggcagcag atgcagctct ttcagagggc tacgtaatct ctgataaaaa tcaatctgcg
    10681 gttggcgatg caccacatag tcatcacctg atatcagaag ttgcgtgggg agcgtaatcg
    10741 ccgccgcatc tctaataata cgttcagacg ttttatagag atcgagcaag atattgacgg
    10801 caatcgcccg tgtgatcagc ggatcattat tgaaactcgc cacccgttgc cgatcgtggg
    10861 tcaaatagcg tcctttcaca taggaattaa taaaaaacag accacgcaga cgatgccata
    10921 acgccagcgc aggacgtgcc agcggcacat acaatttaac cttaaaggcc ggagaagcca
    10981 gcaccagccc gcgaattgca ggcgcataat catgaaccta tgtggcaacc agcactgcgc
    11041 cgacgctttg cgcgatcaca accacctctt ccagtccaac ctagctgtcg ctggcagcaa
    11101 aacggacaaa ttcatcgaca tcctgcactg aacgcgcaag agatgggcta taaccacgcg
    11161 gccccgaagt ttttccatgc ccccgagcat cccatgcata aaaagcagta tctggcatcg
    11221 ccagttcatc aacgatatgt tgtagacgac cagaatgttc atggccacga tgaaataaga
    11281 caatgacctt tttcgcccct g
//
LOCUS       DAJVUJ010000137        11238 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.137, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000137 DAJVUJ010000000
VERSION     DAJVUJ010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11238
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.137"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            86..523
                     /locus_tag="OQQ48_003243"
     CDS             86..523
                     /locus_tag="OQQ48_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HCS1953913.1"
                     /translation="MKTIKPESIALFCLLSGDVDLAKHLADILPITCFVGEDWQQDFQ
                     PLDGGPVQSLSDSYSDYSVLLLIAAESEISVPEWLAHSEGTTVILIAEHNARLLTGPA
                     GGAVALIHYLNEICHSRETAVNDAFCRTLTRGSLAIPQCHLLS"
     gene            657..1201
                     /gene="cobU"
                     /locus_tag="OQQ48_003244"
                     /pseudo
     CDS             657..1201
                     /gene="cobU"
                     /locus_tag="OQQ48_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
     gene            1198..1941
                     /gene="cobS"
                     /locus_tag="OQQ48_003245"
     CDS             1198..1941
                     /gene="cobS"
                     /locus_tag="OQQ48_003245"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HCS1953914.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILALLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            1953..3032
                     /gene="cobT"
                     /locus_tag="OQQ48_003246"
     CDS             1953..3032
                     /gene="cobT"
                     /locus_tag="OQQ48_003246"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HCS1953915.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGVPHVGKKAVLVMCADHGVWEEGGAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVYVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRHQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRAPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            3094..4029
                     /gene="ldtA"
                     /locus_tag="OQQ48_003247"
     CDS             3094..4029
                     /gene="ldtA"
                     /locus_tag="OQQ48_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCS1953916.1"
                     /translation="MMRRVNIFCSFALLFASHTSLAVTYPLPPEGSRLVGQSYTVTVP
                     DHNTQPLETFAAQNGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSNWLEVHEPLSRNRAEYEFDRKVPLPVTPSLR
                     AFISGQEVDVNRANAALQRRSGMPVPISSGSRQMF"
     gene            complement(4085..4160)
                     /locus_tag="OQQ48_003248"
     tRNA            complement(4085..4160)
                     /locus_tag="OQQ48_003248"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4125..4127),aa:Asn,seq:gtt)
     gene            4487..4858
                     /locus_tag="OQQ48_003249"
                     /pseudo
     CDS             4487..4858
                     /locus_tag="OQQ48_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416493.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            4857..5291
                     /locus_tag="OQQ48_003250"
                     /pseudo
     CDS             4857..5291
                     /locus_tag="OQQ48_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Catalyzes the hydrolysis of AMP to form adenine and
                     ribose 5-phosphate using water as the nucleophile;
                     incomplete; partial in the middle of a contig; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
     gene            5634..6350
                     /locus_tag="OQQ48_003251"
     CDS             5634..6350
                     /locus_tag="OQQ48_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCS1953917.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGSDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
     gene            complement(6806..6881)
                     /locus_tag="OQQ48_003252"
     tRNA            complement(6806..6881)
                     /locus_tag="OQQ48_003252"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6846..6848),aa:Asn,seq:gtt)
     gene            complement(6982..8625)
                     /gene="yeeO"
                     /locus_tag="OQQ48_003253"
     CDS             complement(6982..8625)
                     /gene="yeeO"
                     /locus_tag="OQQ48_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HCS1953918.1"
                     /translation="MLRHILTAKNLLSNPIFKFPNCLPFLSTVCCICRQFVGENLCSF
                     ADSPSLFEMWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITPLAVPIF
                     MENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVVVAFSLG
                     KRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKVLALTYLEL
                     TVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSWPGLGFV
                     GAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIGIPASVE
                     SVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITGRRLGVG
                     QIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILIWLNALF
                     MPIWSASWGLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVGVWMGMF
                     ADWAVRAVLFYWRMVTGRWLWKYPRSEPQKCEKKPVVSE"
     gene            8630..8705
                     /locus_tag="OQQ48_003254"
     tRNA            8630..8705
                     /locus_tag="OQQ48_003254"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8663..8665,aa:Asn,seq:gtt)
     gene            8867..10129
                     /locus_tag="OQQ48_003255"
     CDS             8867..10129
                     /locus_tag="OQQ48_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCS1953919.1"
                     /translation="MSLNDSKIRNLKPSSKPVKLSDSHGLYLLVNQGGSRIWYLKYRF
                     SGKESRVSLGAYPLVSLAEARQRRDDIRKLLTQNINPAHQRMSDKAAASPEKYFKAVA
                     LAWHKTNKKWSPDYAARILASMENHIFPAIGHLPVTTLKTQNFTALLRVIEDKGFLEV
                     ASRTRQQLCNIMRYAVQQGFTENNLAQHLEGVTAPPVKNHYPALPLERLPELFERIGD
                     YQQGRQLTRLAVVLTLHLFIRSSELRFARWGEIDFRNKIWTIPATREAIEKVRFSGRG
                     AKMRTPYIVPLSRQAIAILKQIKEISGHLELVFPGDHNPYKPMSENTVNRALRLMGYD
                     TKTDVCGHGFRTMACSALVETELWSRDTVERQMSHQERNSVRAAYIHRAEHLDARTAM
                     MQWWSDYLDVSREGYVAPYIYARRHKAA"
     gene            complement(10848..>11238)
                     /locus_tag="OQQ48_003256"
     CDS             complement(10848..>11238)
                     /locus_tag="OQQ48_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="integrase domain-containing protein"
                     /protein_id="HCS1953920.1"
                     /translation="LERGEPRLTVVFGTKGGRPRETVILDAVAIRKALDNALVVAEDR
                     HGRLIDKPDLKSAMKYWHSQASRLGLTGTYSPHSLRYAWAQDAIRHYLAQGFSEKEAL
                     AMTAMDLGHGDGRGRYVAQVYGRKDTD"
BASE COUNT         2992 a         2614 c         2742 g         2890 t
ORIGIN      
        1 gggctgcatt caaggtgtgg caatattgtt tcatggctta tcaaccagag cgccattaca
       61 ggaaattaag tggagggcga gaggcatgaa aacgataaaa cccgaatcga tagctctttt
      121 ttgcctctta tctggagatg tggatctggc gaaacatctg gctgatatat tgccaataac
      181 ctgttttgtt ggtgaggact ggcagcaaga tttccagcct cttgatggtg ggcctgtgca
      241 atcgttatca gattcgtata gcgattattc agtgctgcta ttgatagcag ctgaaagcga
      301 gatatccgta ccagaatggc tcgctcattc tgagggaacg acggtaattt tgattgcaga
      361 acacaatgcc cgtttgctga ctggtccggc tggtggggct gtagcattaa tccattatct
      421 gaatgaaatc tgtcattcca gagaaaccgc tgtcaatgac gcattttgtc ggacgttaac
      481 acgaggaagt ctggctattc cgcaatgcca tcttctttcg taatggattg agaatgctgg
      541 atttttaatt atattacgcg cagtattaat cgcgtaaatg ttttgcggtc agcaacggtg
      601 ggggatccat tgttctgttc aatgaatacc atcatacaat tgaatcagga gtcggtatga
      661 tgatttcggt gacggggggc gtacggagcg ggaagagtcg ccacgcagag gcgcttattg
      721 gggactcttc acaggttctg tatacgccac ctcgcaaatc cttgatgatg agatggctgc
      781 acggatagaa catcatcggc aaggccgccc gacgcactgg cacacagtgg agcgctggca
      841 acatcttgat gaattaattc atgcagacat taacccgcat gaggctgtat tgctggaatg
      901 cgttaccacc atggtgacta atctgttgtt tgattatggc ggcgataaag accctgatga
      961 atgggattat caggcgatgg aacaggcgat taatgctgag attcagtcgt tgattgctgc
     1021 ctgccaacgt tgtcccgcaa aggttgtatt agtgactaac gaagtgggaa tggggattgt
     1081 gccggagagt cgtctggcac gacattttcg tgatattgcc gggcgggtaa atcagcagct
     1141 ggccgctgcg gcaaatgaag tatggttggt ggtttcgggt attggagtaa aaatcaaatg
     1201 agtaaattat tttgggcgat gctctctttt attacgcgtc tgcctgtacc gcgtcgctgg
     1261 tcccagggac tggatttcga gcattattct cgcggtatta ttacttttcc tttaattgga
     1321 ttattgcttg gcgcgattag cgggctggtc ttcatggtgc tgcaggcatg gtgtggcgta
     1381 ccactggcgg cactgtttag cgtacttgtg ctggcgctga tgaccggggg attccacctg
     1441 gacggcctcg ccgatacctg tgatggcgta ttttctgcac gtagccgcga tcgcatgttg
     1501 gaaatcatgc gtgatagccg tttaggcacc cacggcggtc tggcattgat ttttgttgta
     1561 ctggcaaaga ttctggtatt aagcgagttg gctctgcgtg gcgaaccgat ccttgcattg
     1621 ctggcggcag catgtgcggt cagccgcggg actgccgcat tattgatgta tcgtcatcgt
     1681 tatgcccggg aagaaggact tggcaatgtg ttcattggca aaattgatgg gcgacaaacc
     1741 tgcgtcacac tcggcttagc cgctattttt gctgccgtat tattgccggg tatgcatggt
     1801 gtggctgcta tggtggtgac gatggtggcg attttcatcc tcggtcagct tctcaaacgt
     1861 acgctgggcg ggcaaaccgg cgatacgctg ggagctgcta tagagcttgg tgaactggtc
     1921 ttcctgctgg cactgctata aagagacgtg acattcaaac acttgccgat ttactgaata
     1981 cgatccctgc tatcgatcct gccgctatgt cgcgtgcaca acggcatatc gacgggttac
     2041 tcaaacctgt tggtagcctg ggaaggctgg aggcgcttgc catacaactg gcaggaatgc
     2101 cggggttgaa tggcgttccg catgtgggca aaaaagcggt actggtcatg tgtgccgatc
     2161 acggcgtctg ggaggaaggg ggcgctattt ccccaaaaga agtgacagcc attcaggctg
     2221 aaaatatgac ccgcggaaca accggcgtgt gtgtgctggc agcacaagcg ggcgctaacg
     2281 tctacgtaat tgatgttggt attgatactg ctgagcctat ccccgggctt atcaacatgc
     2341 gtgttgctcg aggtagtggc aatattgctt cagctccggc aatgagtcgc catcaggcag
     2401 aaaagttgct tttggacgtc atatgttata cgcgggagct ggcaaaaaac ggtgttacgc
     2461 tgtttggtgt aggtgaactg gggatggcaa acacgacacc ggcagcggca atagtcagca
     2521 caatcactgg ccgggctcct gaagaagtgg ttgggattgg cgcaaacctg ccgacagata
     2581 aactggctaa taaaattgat gttgtgcgcc gggcgattac gttgaatcaa ccaaatccgc
     2641 aggatggcgt tgatgtcctg gcaaaagtgg gtggatttga tttggtcgga atggctggcg
     2701 tgatgttagg cgctgcgtcc tgcggtttac ccgtgttgct ggatggattt ctttcttatg
     2761 ctgctgcgct cgcagcctgt cagatgtctc ctgcaatcaa accgtatctc attccttctc
     2821 acctgtcggc agaaaaagga gcgcgtatag cgctgtcgca tttggggctg gagccttatc
     2881 tcaatatgga gatgcgttta ggtgagggga gtggcgcagc tctggcgatg cccatcatcg
     2941 aagctgcctg tgcgatatat aacaacatgg gcgaacttgc tgccagtaat attgttctac
     3001 cggggaatac gacttctgat ttgaacagtt aaaaacgaaa agtctgataa aagctatact
     3061 taacggatag ctttcgcgac ttaggaaagg gacatgatgc gtcgtgtaaa tattttttgc
     3121 tcatttgctc tgctttttgc cagccatact agcctggcgg taacttaccc attacctcca
     3181 gagggtagcc gtttagtggg gcagtcgtat actgtaactg ttcctgatca caatacccag
     3241 ccgctggaga cttttgccgc acaaaacggg caagggttaa gtaacatgct ggaagcgaac
     3301 ccgggtgctg atgttttctt gccgaagtcc ggctcgcaac tcaccattcc gcagcaactg
     3361 attttgcccg ccactgttcg taaagggatt gtggttaacg tcgctgagat gcgtctttat
     3421 tactacccac cagacagtaa tactgtggaa gtctttccta ttggtatcgg ccaggctggg
     3481 cgagaaaccc cgcgtaactg ggtgactacc gttgaacgta aacaagaagc tccaacctgg
     3541 acgccaacgc cgaacactcg gcgcgaatat gcgaaacgag gggagagttt gcccgcattt
     3601 gttcctgcgg gccccgataa tcccatgggg ctgtacgcga tttatattgg caggttgtat
     3661 gccatccatg gtaccaatgc caattttggt attgggctcc gggtaagtca gggctgtatt
     3721 cgtctgcgca atgacgatat caaatatctg tttgataatg ttcctgttgg gacgcgtgtg
     3781 caaattatcg accagccagt aaaatacacc actgaaccag atggctcgaa ctggctggaa
     3841 gttcatgagc cgttgtcgcg caatcgtgca gaatatgagt ttgaccgaaa agtgccattg
     3901 ccggtaaccc catctttgcg ggcgtttatc agcgggcaag aagttgatgt aaatcgcgca
     3961 aatgctgcgt tgcaacgtcg atcgggaatg cctgtgccaa ttagttctgg ttcaagacag
     4021 atgttttaag agcgttggta attagaaagc aaaaagcctg ctagaaagca ggcttttttg
     4081 aatttggctc ctctgactgg actcgaacca gtgacatacg gattaacagt ccgccgttct
     4141 accgactgaa ctacagagga atcgtgtgaa cggggcgcat attaatggca gtgcatggtg
     4201 atgtcaaagg gtgaaatgca tttttttgtt tgtttgctgt caatttcaac aaagtggtga
     4261 gctttcaatc ttattgggta atgaaccatc gtggtgcata ccctcctttt atagggcagg
     4321 ggaacgcgac agctgattaa aggagcaaat gacgaatcta tcataatctc ttatttgaca
     4381 gttggttagc ttgtacatca acgccaaaat aaaactggca agcatcttgc aatctggttg
     4441 taagtaatgg cggcacttgg gccgattctt aaaaaccgga ggcaacatga acttcagacg
     4501 cctgaaatac ttcgtaaaaa ttgtagatat tggtagcctg acccaggctg ctgaagtatt
     4561 gcatatcgca caaccagcgc tcagccagca ggttgccaca ctggaaggtg agttaaatca
     4621 acaacttttg atccgtacaa agcggggcgt tacgccaaca gacgccggaa aaattctcta
     4681 tacccatgcg cgggccattt tacgtcagtg tgaacaggcc caactggcgg tgcataacgt
     4741 tggtcaggca ttatcggggc aagtctcgat tggctttgca ccaggaaccg ctgcgtcatc
     4801 catcaccatg cccttattac aggcggttcg cgctgaattt ccggagatcg ttatctatcc
     4861 cgagcattgc tgaagtgcaa cgtgcgcttt atgacgccac caagctggtg agcggcaggc
     4921 ccggtgagga agtcaaacag cggctacgta ctggtactgt ggtaaccaca gatgacagga
     4981 actgggaatt acgttactca gcttctgcac ttcgttttaa cttaagccgg gccgtagcaa
     5041 ttgatatgga aagtgcaacc attgccgcgc aaggatatcg tttccgcgtg ccatacggga
     5101 cactactgtg tgtttcagat aaaccgttgc atggcgagat taaacttccc ggccaggcta
     5161 accgttttta tgaaggcgct atttccgaac atctgcaaat tggcattcgg gcgatcgatt
     5221 tgctgcgcgc agaaggcgac cgactgcatt cgcgtaaatt acgaaccttt aatgagccgc
     5281 cgttccgata ataagttctg aaccttctta tcagaaatag atgtgaagga gtaagtaaga
     5341 cacctggcaa atagcctgca ataatcgtgg gctgtttgct tccttgggcg gatacgtgtt
     5401 ttattatcgt cttaatgatt tccacatatt aaaagcaagt atgctttcaa aacacaatta
     5461 taaaaaatcc cgccaacaat ataagttttt ataaaattaa atataagatt atggctttag
     5521 aatattttta tttctaatag acgagatttt tcctgttatg atataatatg ccgaattaac
     5581 acatgttaac gatttaccag taatgtaaat aaattttcga ggagatcatt ccagtgggac
     5641 gtaaatgggc caatattgtt gctaaaaaaa cggctaaaga cggtgcaacg tctaaaattt
     5701 atgcaaaatt cggtgtagaa atctatgctg ctgctaaaca aggtgaaccc gatccagaat
     5761 taaacacatc tttaaaattc gttattgaac gtgcaaagca ggcacaagtt ccaaagcacg
     5821 ttattgataa agcaattgat aaagccaaag gcggcggaga tgaaacgttc gtgcagggac
     5881 gttatgaagg ctttggtcct aatggctcaa tgattatcgc cgagacattg acttcaaatg
     5941 ttaaccgtac gattgctaac gttcgcacaa ttttcaataa aaaaggcggc aatatcggag
     6001 cggcaggttc tgtcagctat atgtttgaca atactggtgt gattgtattt aaaggatcag
     6061 accctgacca tatttttgaa attttacttg aagctgaagt tgatgttcgt gatgtgactg
     6121 aagaagaagg taacattgtt atttataccg aacctactga ccttcataaa ggaatcgcgg
     6181 ctctaaaagc agctggaatc actgagttct caacaacaga attagaaatg attgctcaat
     6241 ctgaagttga gctttcccca gaagatttag aaatctttga agggcttgtt gatgcccttg
     6301 aagatgacga cgatgtacaa aaagtttatc ataacgtcgc aaatctctaa ttatatatta
     6361 aagaaatctg tctttatggc agatttcttt aatctcatat aatccttata aaaaatataa
     6421 tgttcaactc gtcatattgc ttataaaaaa tcctcgttcc cagagaaata atatttatta
     6481 taattccagt tcttcttttt ctgattacag aaggcaaagt gacaattacg catagtttcc
     6541 cgataaagac gcgacagcta catcccgcat aaggcatttt tctctttatc cttgtacggt
     6601 acttcatgga acagagtttt tgaccttgcg aatcgtgatg tctgttgggg aggggcaatt
     6661 tgctcactga agcgtgagac tcgattaagc gcacgaaaca cagaaatcaa aaaacccggg
     6721 cactttttta caaggtaacc gggtaaaaat aatttttatt ttttaactgt tttgagactc
     6781 atagagatgt ctcaaaacta aaatttggct cctctgactg gacttgaacc agtgacatac
     6841 ggattaacag tccgccgttc taccgactga actacagagg aatcgtgaga acgaggcgaa
     6901 tattagcgat gcccacccac aatgtcaaag cctgttattt aaatttgaaa tcgtttgctg
     6961 aaataatctg cattttgtcg tttattccga cacaactggc tttttttcac acttttgcgg
     7021 ttcggatcga gggtatttcc acagccaacg tccagtaacc attcgccagt aaaacagcac
     7081 ggcccgcaca gcccagtcgg caaacattcc catccagaca ccaaccacac cccagccaag
     7141 catgatgccc agcacataac cgacgacaac ccggcaaccc cacatgctca acatagaaac
     7201 ccacatggcg taacgggcat cgcgagcacc tttaaatcca gcgggtagcc cccatgaggc
     7261 ggaccaaata ggcataaata aagcatttag ccaaatcaga atcacaacga catgtttaac
     7321 ctgtggatcc tgggtgtaaa acgatgccat aaccccggca aagggagccg ttagccaggc
     7381 gatggccgtt aatccaagag tggaaagcca gaacacatgc cgcaactgaa tctctgcttg
     7441 cgctatctgc cctaccccca accttcggcc tgtaatgatc gtagaagcag agccgagcgc
     7501 acttccgggt aagttgataa gagccgcaat tgaaaacgcg ataaaatttc cggcaataac
     7561 actggtcccc atcccggcaa cgaacatttg ggttaataac cgaccactgg taaataacac
     7621 tgattcgaca ctcgcgggaa taccaatccc catgacttcc cagataatgc taaaattcag
     7681 cggtttaaaa tagctcttta acgaaatcct tagcgcagga ttaaaaccaa tcgccagcac
     7741 ccacaaaatt gcaactgcgc caatataacg agaaatggtt aaacccagcc ctgccccgac
     7801 aaatcccagt cccggccagg agaaaaggcc gtaaatcaat atgccgctaa taataatatt
     7861 aagaatattc aggctaccgt taatcaatag cggtattttc gtattccctg caccacgaag
     7921 tgccccgcta ccaataagag tgatggcagc tgctggataa ctgagtaccg tcagctccag
     7981 ataagtcaac gccagtactt taacttctgt cgtggcatca cccgcgacga aatcaataat
     8041 ttgttcgcca aaatgatgaa taagcgttgc caacagtacg gcaaacaacg tcatgatcac
     8101 caatgactgc cgcgtcgcca ccctcgctcg tcgtcgatcc cgcttaccga gactaaatgc
     8161 cacaacgaca gtagtaccaa gatcgatagc agcaaaaaaa gccataatga ccatattgaa
     8221 gctgtccgcc aatcccacgc cggccatcgc atcttttccc agccagctga ccagaaaagt
     8281 gctcagaacc cccatcaaca ggacacaggc attctccatg aagataggaa cagcaagcgg
     8341 ggttatctcg cgccagaaca acactttgta gctcttgcgt ttagcgtgcc agcgagtgcc
     8401 gtgaacaacc tggcgtaaag cagaggagat attcaaagcc gaccttaatt gcagaaagtg
     8461 aaaccacatt tcaaataatg agggagaatc agcaaagctg caaagatttt cgccaacaaa
     8521 ttgtctgcaa atgcaacaaa ctgttgatag aaacggcaaa cagttgggga atttaaaaat
     8581 cgggtttgac aaaagatttt tcgccgttaa gatgtgcctc aacagcgatt cctctgtagt
     8641 tcagtcggta gaacggcgga ctgttaatcc gtatgtcact ggttcgagtc cagtcagagg
     8701 agccaaattc cgattcttac gctatcttat tagtccttat ttgatattga ttcaataagt
     8761 tagcgtgaaa atctttccag atgtgtattc agttttccct ccgcatccgg ttaatttggt
     8821 ggtcagattt gggggcaggt tggttcgatt ctggagtgac ccccaaatgt ctttaaacga
     8881 ttcaaaaatc cgtaacttaa aaccctcttc aaaacccgta aaactgtctg attcgcacgg
     8941 tctgtatctg ctggtcaatc agggcggttc gcgtatctgg tatctcaagt atcgcttcag
     9001 tggtaaagag tcccgcgtca gtctgggcgc atatcctctg gtttcacttg ctgaagcaag
     9061 acaacgacgt gacgatatcc gtaagctact gacgcagaat attaaccccg cccaccagcg
     9121 catgtctgat aaagccgcag cgtcaccgga aaagtatttt aaagccgtgg cgcttgcatg
     9181 gcacaaaacc aacaaaaaat ggtcgcctga ttatgccgcg cgcatcctcg ccagcatgga
     9241 aaatcatatc tttccggcga ttggtcatct gcctgtcact acgctgaaaa cgcagaattt
     9301 cactgcgtta ctgcgggtta ttgaggataa aggttttctg gaagtcgcgt cccgaacccg
     9361 gcagcaactc tgcaacatca tgcgttacgc cgtacagcag ggattcaccg aaaacaacct
     9421 ggcgcagcat ctggaaggcg tcaccgcgcc gccggttaag aatcactatc ccgccctgcc
     9481 gctggagcgg ttgcccgaat tgtttgagcg cattggcgac tatcagcagg gacggcagct
     9541 tacgaggctg gcggtggtgt tgactctcca cctgtttatc cgttccagcg agctgcgctt
     9601 tgcccgctgg ggtgagatag atttcagaaa caaaatctgg accatccccg ccacaaggga
     9661 agcgattgag aaagtccgtt tctctggccg tggcgcaaaa atgcgtactc catatatagt
     9721 gccgctttct cgtcaggcta tcgccattct gaagcagata aaggaaatct ccggccatct
     9781 ggaactggtg ttccccggcg accataaccc gtataagccg atgagcgaaa acactgtcaa
     9841 ccgggcgttg cgtcttatgg gctatgatac caaaactgat gtctgtgggc atggattccg
     9901 gacaatggcg tgtagcgcat tagtggaaac tgaactctgg tcacgggata ccgtggagcg
     9961 gcagatgagt catcaggagc gcaacagcgt tcgggcagcg tatatccacc gggctgaaca
    10021 tctcgacgcc cgtacagcca tgatgcagtg gtggtcggat tatctggatg taagtcgcga
    10081 ggggtacgtt gcgccgtata tttatgcgcg gcggcataag gcggcctgag acagtggcta
    10141 tataatttgt taaaaatgaa aaagcagatc ctgattgaat ccggatctgc tttttacatt
    10201 aggcaatcgc tgccagacaa tctgcaccag aaacgccata actgcaccag ttcagtacca
    10261 ccgcttacct gcgccagtct gccgtcgcct gtctgtgtca ggactgcgcc agatcttatc
    10321 gaataaacat caaactgcgc cagtgttacg gccctttcag ccctttggcc ttttccggtc
    10381 tggcgctgtt acccttagcc gccccttacc ctttccggcc tttttccttt gctggcgcaa
    10441 tcgccagaaa aatcgtcaag ctgcaccaga agcctgccaa taggtcatac cgttcagaag
    10501 catcgttatt gagaggaaac cgtacaaaaa atttctacgt agaaattttt tcggtggata
    10561 acgatgaata aagagtattt tataatgtga tggctatcaa aaatcgacca tccaaaaata
    10621 attcattgtt cattgattta taatgaataa ccatgaattt aatagtcgat gattattgtt
    10681 tttatcagta ttgataggaa agtatacaaa aaattttggc cccaaaattt tttgtacgaa
    10741 aaccgatgaa taacgcgaaa ttaataggaa accatccaaa attatttggg gcaaataatt
    10801 ttgcccgaaa accgatggat aactcgatct tttcaaagca acggctatca atcagtatcc
    10861 tttcttccgt aaacctgcgc cacataccgc ccgcgcccgt cgccgtgtcc caaatccatc
    10921 gccgtcattg ccagcgcttc tttctcactg aatccctgcg ccagataatg acgaatggca
    10981 tcctgcgccc acgcatagcg caacgagtgc ggggaatatg tgccagttaa cccgagacgt
    11041 gacgcctggc tgtgccagta tttcatcgcg cttttcaggt cgggtttatc tatcagcctg
    11101 ccgtggcgat cttctgccac aaccaacgca ttatccagcg cttttctgat agcgacagca
    11161 tccagaataa cggtttctcg cggacgcccg cctttggtgc cgaacaccac ggttaaacgg
    11221 ggctcacccc gctccagc
//
LOCUS       DAJVUJ010000138        11227 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.138, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000138 DAJVUJ010000000
VERSION     DAJVUJ010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11227
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.138"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            63..662
                     /locus_tag="OQQ48_003257"
     CDS             63..662
                     /locus_tag="OQQ48_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCS1953921.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNALVCAGCEQIFEDKLSGTKTDRPG
                     LKRALKRLQKGDTLVVWKLDRLGRSMKHLISLVGELRERGINFRSLTDSIDTSSPMGR
                     FFFHVMGALAEMERELIVERTLAGLAAARARGRTGGRRPKLTKEQHEQIARLIKNGHD
                     RKQLAIIYGIGTSTIYRYHPVGDIQTEETTGQTQENENR"
     gene            complement(691..1179)
                     /locus_tag="OQQ48_003258"
     CDS             complement(691..1179)
                     /locus_tag="OQQ48_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001761054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1953922.1"
                     /translation="MMMIYGMFVFELRTLPHQQLQQNKSWRHVKNERVNRSASWQYIG
                     AGDDRIVLSGVLYPEITGGEVSLSLLTTQAYTGRPWPLIDGVGQIYGMYVLTETNTTR
                     SEFDRYGKAKKIEFSLTLERCDEDLRERLQSSSFSDMLSGFKDKVTSSLNSAASSVKG
                     LF"
     gene            complement(1192..3999)
                     /locus_tag="OQQ48_003259"
     CDS             complement(1192..3999)
                     /locus_tag="OQQ48_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCS1953923.1"
                     /translation="MKTLDIRVAFSAVDRLTRPAENARRLMGQFGDSIQRTQGAIKNL
                     ERQARSFERARDAVSKADAGIVKARRQLNALNQLQRTGTVLSEKQQKLMQQLSTRLER
                     LNESRTREIQKMRELGGELKRHGISLTGSDNTIQQAIRRTEQYNNQLERERQALARVT
                     RARERYSRAQETAGKLKTGGALAIGAAAAGGYAAGRFLQPAIGFGKEMSRVQALTRID
                     QNSPQFKALREQALKLGSETQFTAGDAASGQAFLAMAGFTPQAIQAALPGVLSMATAG
                     GMDLGETADIGSNILTQFGLSADQMDRVGDTLTAAFTRTNTDLRALGETMKYAGPVAG
                     KLGISLEQAAAMAGVLANMGIRGSDAGTAMRASLARLASPPKAAAEALKELGVSVSDA
                     GGKMRPMEDVLADLYKATRKYGEVDRVSFFKDIAGEEAFTSFMALVDAAGDGSLPKLR
                     KELEGARGEAERTAKVMANNLDGDLKSLSSAWEGLRIRIADLIDGPLRSVTQWLTRVV
                     SKVTALAQAHPALTRQLLIAGGALLAMTATVGSLSLAIGVLAGPLAKLRLGFSLLTGS
                     MNAVRVLPALWGMVTGSVSLLGGAIGALFSPVGLIVAALAGAAVLIWKYWDPIRAFFA
                     GVFSGIMERLTPLRETFERFGPVFDAIGSGISQVFNWFKSLLSPMESSKETLDKCTSA
                     GEIFGNVLGGALQLVLTPAKMLLDTLAWILEKLGVLPDEAERARKKIEDAQRAAILQD
                     KVALLQGDLAKINPPKPVENGNGTGGDNPKDNKPLTDSNTGTLRRLSKIADNTGKLVE
                     ETKKRIGPGDIVFKNLPRALAVRGEWQERKIAQVSKPAPAINITPVVPAPLPPALVPV
                     VAASSRPVAEAIRSPVASVPVTSRNREPVAFGFGGEIHVHLHNVVTQNPRELAKLVGE
                     MVRAEMERRARAGRGCFYDKD"
     gene            complement(4037..4141)
                     /locus_tag="OQQ48_003260"
     CDS             complement(4037..4141)
                     /locus_tag="OQQ48_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HCS1953924.1"
                     /translation="MCFEHIEDLVADIAAIFNWSPAEIFMMTPGEVVS"
     gene            complement(4150..4524)
                     /locus_tag="OQQ48_003261"
     CDS             complement(4150..4524)
                     /locus_tag="OQQ48_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1953925.1"
                     /translation="MKDIDTETRNNTVADNVTAVEDMAVERGVKLTRPIERGGEKITY
                     VEITGAIEQAGSLRGLSLSDVLNLKADTMFTLLPRVTSPRLDEVMIKKMSSRDFIQLC
                     AVAVNFMSEPDSGAKSVQETAA"
     gene            complement(4580..5090)
                     /locus_tag="OQQ48_003262"
                     /pseudo
     CDS             complement(4580..5090)
                     /locus_tag="OQQ48_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310671.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
     gene            complement(5090..6274)
                     /locus_tag="OQQ48_003263"
     CDS             complement(5090..6274)
                     /locus_tag="OQQ48_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HCS1953926.1"
                     /translation="MSETRFHGARVTENTDLVTAINDVDSSVIGIVATADDADAKLFP
                     LNKPTLLTRVNDVLGKCGTTGTLYRALKAIADQVSTKVIVVRVAEHKEEDGKTQDQLV
                     IGGSEDDGSYTGMYALLVAEQDESIGYRPRILAAPELDTEAVTKSLCVIADKLRAFVY
                     ASCHGCNTMAEAITYRQKFNEREVMLLWPDFIAYNPKNGKNETFPAPAYACGLRAYID
                     HEQGWHKSLSNVPVKNVLGMSRHVFWSLQAEDSDANSLNNKEITTIIRRNGFRFWGNR
                     TPETNAYIFEVYTRTAQVLADSIAEAQFETIDSPLTPANVKDVISAIRAKLDSLVTAG
                     KLIGAECWYDVVDNSTTDLRQGRVRIRYKYTPVPPLEDMELYQTFTDEYFEPAFAVLG
                     GA"
     gene            6432..7541
                     /locus_tag="OQQ48_003264"
                     /pseudo
     CDS             6432..7541
                     /locus_tag="OQQ48_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494339.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
     gene            7790..10909
                     /locus_tag="OQQ48_003265"
     CDS             7790..10909
                     /locus_tag="OQQ48_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1953927.1"
                     /translation="MIGQFLSATEILAKNYVRNKMVKNPFYSNLKWNFIEKNIIRLTS
                     SPVKSVLCISAFSFVLLYVGYLNELFIKNNLLHYFPFRHSLTEWQTTILSGQLTIIGI
                     VYPLVIGLVSVLFQKKADRKIAQTAYQRYSGFMLAGLSGLFLSGFILLSVLIKTVFGS
                     YLYGIACLISILWLLINIVLSIWFFIVSLEILDDVKRQIIIKRYIAFEIVMPHICNKI
                     SAKLRLYPIYQKHNYSNLEITQADYKGEYISVASSYSKEDELSLYHRPFQLTLNLINY
                     QLKKKNHFASFVIGDNRTKETESTGKILFSVKNIKPDSLLIKILKQCFYRAPIKGGDF
                     SVSLTMQAITADTYMYLRDSDLISFDNAISALINNFNNLCDLYFFQDDNTNNNFLLIT
                     TELFERSFQYEFSDEVYKISNNSMDKINLSERFFELCLWSGVRIINNRKHLISNELCI
                     YMGITRSQWSILTEWFRNNQSLLNASLRSRYNRILRTYITVWEQYQESINFRFCNTEN
                     SDLFELFCKTQLQELPSMIIDATQTRDPSTIDTAVDLINRWQHSMNIDSHSVEKYSYK
                     GQLFNPGFFISKKLNFNSDREWFNIAIINALTDMRICTCLYLTSRINTSDKLMTHYIK
                     LILEGKLIDQTGGYETPTEEIDNASQLIKILIRICLWTWSENMEHNGWMNSLARRLRD
                     YDKTDMVMGRVYSNVFDCGFIDMEQSWVQLLLIFSKKNDSVSKEIKEAIENNYITYRE
                     KQRLIGVLSKICNSIEYTKIKLTLTLDDLQTKKENLRKLLQEHINMLKKDLDMRLQDA
                     AIDVHRLDSTARKTSEHLRKRIKKTLPLSLFKSIDFKQASDCFTKHKISIKIDKEPYA
                     EGIESIPYINEGDIQADLILKDIQRIILSNLFSTGCSQHTVIEDFNMLIDHIKSSADL
                     AGKLVLVMSKEIFQQYNRMLFDNPNLRELMRKNDDGSMNITTESGTRKVYFLPFVNQP
                     FSLVVKDNYFTKLIIREYDNNKLVNVTSENIKSDSDKFKLTLNYELNIVFEGNADLKI
                     AHSQRVTSE"
     gene            11045..>11227
                     /locus_tag="OQQ48_003266"
     CDS             11045..>11227
                     /locus_tag="OQQ48_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001512982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HCS1953928.1"
                     /translation="MFRCPLCGASARIRTSRPENDSNTVRQKYYQCNNLECGVCFSTL
                     EAFHKFTSKHASPIHSS"
BASE COUNT         3074 a         2815 c         2512 g         2826 t
ORIGIN      
        1 ttaaataatg gccgtttctg gcctgcatgg catgatttgc gcttttggac gggagattca
       61 gtgtgctgat tggctatgta agggtatcaa caaatgacca gaatacagac ctgcaacgaa
      121 acgctcttgt ttgtgcagga tgtgaacaaa tatttgaaga taaattaagc gggacaaaga
      181 cagaccgacc gggattaaaa cgcgctttaa agcgccttca aaaaggtgac acgctggttg
      241 tctggaaact ggatcgcctc gggcgaagca tgaaacatct gatttctctc gtcggggaac
      301 tacgggagcg agggattaat tttcgcagtc tgaccgacag catagacaca tcttctccaa
      361 tggggcgttt tttcttccac gtgatgggtg ccctggctga aatggaacgt gaattaattg
      421 ttgaacgtac actggccggg ctggcggcag cgcgcgcaag ggggcgcaca ggcggacgtc
      481 gaccgaagct gacaaaagaa cagcatgagc aaatagcaag gctaatcaaa aacgggcacg
      541 acagaaaaca actggcaata atttacggca ttggcacatc gacgatttat cgttatcacc
      601 ctgtaggcga tatacagact gaagaaacaa ccgggcagac tcaggaaaat gaaaaccgct
      661 aatctgacca ttagcggttt ttgtgttaaa tcagaacagc cctttaactg aactggccgc
      721 gctgttaaga gatgatgtca ccttatcttt gaagccggac agcatatcac tgaacgatga
      781 ggattgcagg cgctcccgca aatcctcatc acagcgttca agagtcagtg aaaattctat
      841 ctttttcgcc ttaccgtagc gatcaaactc ggagcgggtc gtattcgttt cagtcaggac
      901 atacatgccg taaatctgcc cgacgccatc aatcaaaggc cagggtcgtc ctgtatacgc
      961 ctgcgtagtc agcagcgaca gcgacacttc gccacctgta atttcaggat aaagcacacc
     1021 agaaagaacg atgcgatcat cacctgcacc gatatactgc cagcttgctg aacggttaac
     1081 gcgttcattt ttcacatgcc gccagctttt gttttgctgt aactgctgat gcggcagcgt
     1141 gcgcagctca aaaacaaaca tgccgtagat catcatcatg gccatgactc ctcaatcttt
     1201 atcgtaaaaa cagccacgcc cggcacgggc gcgccgttcc atttctgccc tgaccatttc
     1261 accgaccagt ttcgccagtt cgcggggatt ctgcgtaaca acgttatgca gatgaacatg
     1321 aatttcacca ccaaatccga aggcaacagg ctcccggtta cgggaagtta caggaactga
     1381 tgccactgga gatcgtatag cctccgccac cgggcgggag ctggccgcaa caacagggac
     1441 cagcgccgga ggcagcggag ccggaaccac gggtgtgata ttaattgcgg gggcaggctt
     1501 actgacctgc gcaatcttcc gctcctgcca ctccccacga acagcaagtg ctcggggcag
     1561 gtttttaaag acaatatcgc cggggccaat gcgttttttc gtctcctcaa ccagcttacc
     1621 tgtgttatca gcaattttgc tgagtctgcg cagcgtaccg gtattgctgt ctgtgagcgg
     1681 tttattgtct ttggggttat cacctccggt gccattgcca ttttccacag gcttcggcgg
     1741 attgattttc gccagatccc cctgaagcaa ggcaaccttg tcctgaagaa tggccgcacg
     1801 ctgtgcgtct tcgattttct tgcgcgccct ttccgcttca tccggaagca caccaagctt
     1861 ttcaagtatc cacgccagcg tatccagcaa catttttgca ggtgtcagaa caagttgtaa
     1921 cgcaccgcca agaacgttac cgaatatctc gccagcactg gtacatttat ccagagtttc
     1981 cttgctggac tccatcggtg acagcagcga tttaaaccag ttaaacacct ggctgatccc
     2041 actcccgatt gcgtcaaaaa caggaccaaa ccgttcaaag gtttcgcgca acggggtcag
     2101 cctttccata atcccgctga acaccccggc aaaaaatgcc ctgatgggat cccagtattt
     2161 ccagataaga acggcagctc cggcaagcgc agccacgata agaccaaccg gactgaacaa
     2221 cgccccgata gcgcctccca gtaaagaaac ggaacccgtc accattcccc atagtgctgg
     2281 cagaaccctg acagcattca ttgatccggt caggagggaa aaaccaagac gcagttttgc
     2341 cagcgggcca gcaagcacac caatagccag cgacaacgag ccaaccgttg cagtcattgc
     2401 cagcaatgca ccgcctgcaa tcagtagctg gcgcgtcagt gcgggatggg cctgcgccag
     2461 cgccgtcacc tttgatacca cacgcgtgag ccactgcgtg acagaacgca gcggaccgtc
     2521 aatcagatct gcaatgcgga tgcgcaaccc ttcccatgca ctgctgagtg atttcagatc
     2581 gccgtcaagg ttgttggcca taacctttgc cgtgcgttca gcctcaccgc gcgcgccttc
     2641 aagttctttt ctcagtttgg gtaaggagcc gtcacctgcc gcatcaacga gggccataaa
     2701 tgatgtgaaa gcctcttctc cggcaatgtc cttaaagaac gatacccggt caacttcccc
     2761 gtatttgcgg gtagctttat aaaggtcagc cagcacatcc tccatcgggc gcattttgcc
     2821 cccggcatcc gagacagaca cgccaagctc tttcagcgcc tctgctgccg cctttggcgg
     2881 tgatgccaga cgagccaggc tggcacgcat tgccgtacca gcatcactcc ccctgatacc
     2941 catattcgcc agcacgcccg ccatcgctgc ggcctgctcc agagatattc ccagcttacc
     3001 cgccaccgga cctgcatatt tcatggtttc acccagtgcg cgaaggtcag tgttggtacg
     3061 ggtaaacgct gcggtgagtg tgtcgccgac ccggtccatc tggtcagcag aaagaccgaa
     3121 ctgcgtcaga atatttgagc caatatccgc cgtctcaccg aggtccatac cgccagccgt
     3181 tgccatgctc agtacgccag ggagcgcagc ctgaatggcc tgtggtgtaa agccagccat
     3241 tgcaagaaat gcctgtccac tggcggcatc gcctgcggtg aactgcgttt cagagccaag
     3301 ttttaacgcc tgctcacgca gcgccttaaa ctgcgggctg ttctggtcga ttcgcgtcag
     3361 tgcctgaacg cgggacatct ctttgccgaa cccgatcgca ggctgcaaaa aacgcccggc
     3421 agcatagccg cccgccgctg ccgcaccaat tgccagcgca ccacctgttt tcagttttcc
     3481 cgctgtttcc tgcgcgcgcg aataccgctc acgcgcccgt gttacacgcg caagcgcctg
     3541 ccgttcgcgt tcaagctggt tgttgtactg ttcggtgcgt ctgatggcct gctggatggt
     3601 gttatcgctg cctgtcaggg aaatgccgtg gcgtttcagc tctccgccaa gctcccgcat
     3661 tttctgaatt tcccgtgtgc gcgattcatt caggcgttca agccgggtgc ttaactgctg
     3721 catcagcttt tgttgttttt cgctgagcac tgtacccgtg cgttgtaact gattaagggc
     3781 gttaagctgg cgtcgtgctt tcacgatgcc agcatccgct ttactgacag cgtcacgggc
     3841 gcgctcaaat gatcgcgcct gacgctcgag atttttgatc gccccctgcg ttcgctggat
     3901 ggagtcacca aactgcccca tcaggcggcg ggcgttttcg gcaggccggg tcagcctgtc
     3961 aacggcgctg aaagcgaccc ggatatcaag agtcttcatt gtctgcattc ccgctacgaa
     4021 gtgccgcccg ctcacgtcag ctaaccactt cgccgggcgt catcatgaag atttcggcgg
     4081 gcgaccagtt aaaaatggcg gcaatatccg ccaccagatc ttcgatgtgc tcaaagcaca
     4141 ccagggtgat tacgctgccg tctcctgcac gctcttcgcg ccagagtctg gctcgctcat
     4201 aaaatttaca gccacagcgc acaactgaat aaaatcgcgt gacgacattt ttttaatcat
     4261 cacttcatcc agtcgtggcg aggtcacgcg aggcaacagc gtgaacatgg tatccgcttt
     4321 cagattcagc acatcagaca gcgacagacc acgcagggat ccagcctgct caatagcccc
     4381 ggtgatctcc acatacgtga ttttttcgcc accacgctca attggtcggg tcagttttac
     4441 gccacgttcg acagccatat cctcaactgc cgtcacatta tccgccacgg tgttattccg
     4501 ggtttcagta tcgatgtctt tcatctgttg tctccttttc agtcagaggc gacgcactgc
     4561 gccgccagca tattacttat cagccaagcc caagcgcgga acggatgcga tcgggcacaa
     4621 tgtccttgcc gtccttccgg taaatgaagt tcagcaggtc aatctcccac aacgggcgat
     4681 cgttaacact cagcttgtag taggtgtttt aatggcgtaa gtgtgtgatg tggcttcgcc
     4741 ctgtttggct tcccccatat caatttccgt cacacgtccg cgcatttcga cttcatacag
     4801 gtcgctttct gcatcggtgt agtattcacc cgcaaaacgc agcagcgtgc cgtcaatcgt
     4861 gccgccatac ttaaggaaca gctcacgaac tgcgccccca tgacaaagct cgcatcaagc
     4921 gcggagtcgt ccagaccgag atcaatactt accgcaccca tcatgccacc accccggtag
     4981 ctgtcggttt tgcgcgtcag cttaggcaga gtgacggacg tcacattacc cacttcgttt
     5041 tcaccatcca caaacagcgt aaaaaagcga agatgttttg gcacagccat caggcacctc
     5101 ccagcaccgc aaatgcgggt tcaaagtatt catcagtaaa cgtctggtaa agctccatgt
     5161 cttccagtgg cggaacgggc gtatatttgt agcgaatacg cacacgtccc tgacgtaaat
     5221 ccgtggtgct gttatccacc acgtcatacc agcactccgc gccaatcagt ttcccggcag
     5281 tcaccagtga atccagtttt gccctgatgg cactgataac atccttcaca ttcgcaggcg
     5341 tcagtggact gtcgatggtt tcaaactgcg cttccgcaat tgaatcagcc agcacctgtg
     5401 cggttcgggt atacacctca aagatgtagg cgttcgtttc cggtgtgcgg ttaccccaga
     5461 agcggaaccc gttgcgacga ataatggtcg tgatttcttt gttgttgagg ctgttggcat
     5521 cgctgtcttc ggcctgcaac gaccagaaca catgcctcga catccccagc acatttttaa
     5581 ccggaacgtt ggacagcgat ttgtgccatc cctgttcatg gtcaatgtac gcacgaaggc
     5641 cgcacgcata ggcaggcgcg gggaacgttt cgtttttgcc atttttcggg ttgtaggcga
     5701 tgaagtccgg ccataagagc atcacctcac gttcgttgaa tttctggcgg taggtaattg
     5761 cctcggccat cgtgttacag ccatgacatg aggcatacac aaacgcgcgc agtttatctg
     5821 caatcacgca cagggatttt gttaccgcct ccgtgtccag ctccggcgcg gccagaatac
     5881 gcggacggta tccgatgctt tcatcctgct ctgcaacaag cagcgcatac atccccgtat
     5941 agctgccgtc atcctcagaa ccaccgataa ccagttgatc ctgcgtcttt ccgtcttctt
     6001 ctttgtgttc agccacgcga acgacgatca cctttgtgct cacctggtct gcgatggcct
     6061 taagcgcacg ataaagcgtc cccgttgtcc cgcattttcc cagcacgtca ttgacgcggg
     6121 tcagcagtgt gggcttgttc agcgggaaca gcttcgcgtc cgcatcatcc gccgttgcca
     6181 cgataccgat aacgctggaa tcaacatcgt taatcgctgt taccaggtcg gtattctccg
     6241 taacacgggc accatgaaaa cgagtttcac tcatagcttc agccccttgt atccgttaaa
     6301 tgattcagca acaatcatca cccaccacgc gcgtaatctc acccctgcgc cgttctcccg
     6361 ccacggcgac aacaaaaagc agtaaccccc tccgcacgca catgcgacca tgccgcacag
     6421 ggagggagca gatgaccgac accaccatgc aattgctcag tcagggcacg gcccccgtga
     6481 aaatgccgga ttttgatatt ctctcggagg gtaaaacgct gtcaggcgtg gcggagcgcc
     6541 tgatgagcct gtaactgacc gacaaccggg gatttgaggc ggaccagctc accatcacgc
     6601 tggatgatgc ggatggtcag ttgcagctac cgccacgggg cgcgcgtctg acggttctca
     6661 ttggctggaa aggggaaccg ctgacagaaa aaggcactta cattgttgat gaaatcgctc
     6721 acgaaggacc gccggacagg ctgactgttt cagccagaag cgcagatttt cgggatgaat
     6781 ttaacgttaa acgtgaggtg tcctggcatg atgtgaccgt tgagcgtgtg gtatccgcca
     6841 tcactcatcg gtacggtctg aaaccgcaaa tcagcgaaat gctgatggat atcgaaatcg
     6901 accacgccga ccagaccgaa gaaagcgaca tgtcctttct tacgcgcatg gcggaaatgc
     6961 tgggagcaat cgccacggta aaaagcggca atctgttatt catcatgcca ggtggtggcg
     7021 tgaacgcaca ggggcagccg ttgccctcgt tcgccattac acgcagcagc ggcgatcgcc
     7081 atcagttccg cattgctgac cgcgaggcgt atacgggggt acgcgcctac tggcttgatc
     7141 ttaattacgg gaaaaagaaa aaagtcagcg tgaaacgccg caaaccgcca aaacccaaaa
     7201 aggagaaaag cagcagtcgg gaaggcgact atatggaagg tgcggaaggc aacgtgtttg
     7261 tgttacgcaa gacttatcag aacgagcagg cagcaagacg cgcagcggcg gcaaagtggc
     7321 agcagctaca acgcggagcc gcatcattct ccatcacgct ggcgcgtggt cgtgcagaac
     7381 tctatcccga aatgcatggc acagtgacag gctttaaaag cgagattgat tatcaggact
     7441 ggactattgc aaaagccgag cacaccattg ataacagcgg ctttaccacg cagcttgaac
     7501 ttgaagcaaa aatcccggaa tggatagcag aaacagaata aatctcttaa aaatattatt
     7561 ttcgagatgt gcttctacac tgacagtaag aacaaaagat aatgtatcag cccactagat
     7621 gtcagaggtt ggggcatcaa ccagatgcta acaataatac ggatgttttt cccacatgac
     7681 agaggaagtc aagtataatc ttcacagtcc atcccgtcca agggaatatg actcaatagt
     7741 aaaagttcca gtttagttag tagacccaca ctttatagaa gagtaattca tgattggaca
     7801 atttttaagt gcaacagaga ttttagcaaa gaactatgtt cgtaataaaa tggttaaaaa
     7861 tccattttat tcgaatttaa aatggaattt tattgaaaaa aacatcataa gattaacatc
     7921 ctcaccggtt aaatcagtac tttgcatatc agctttttca tttgtactac tttatgttgg
     7981 atatttaaac gaattattta taaaaaataa tcttttacat tatttcccgt ttaggcattc
     8041 attaaccgaa tggcaaacaa ccattttaag tggccaattg acaattattg gtattgtata
     8101 cccccttgtt ataggtttag taagtgtctt attccaaaaa aaggcagata gaaaaatagc
     8161 ccaaactgct tatcagcgtt actcaggatt catgcttgct ggactcagcg ggcttttctt
     8221 atcaggattt atacttctca gtgtactaat taaaactgtc tttggaagtt atctttatgg
     8281 tatagcttgt ctaatcagca tattatggct tttgataaac atagttcttt ctatttggtt
     8341 ctttatcgtg agtcttgaaa ttcttgatga tgtaaaacgg caaataatta taaagcgata
     8401 cattgccttc gaaatagtaa tgccccatat ttgtaacaaa atctcagcca agcttaggtt
     8461 ataccctatc tatcaaaaac ataactattc aaatttagaa atcacgcaag ccgattacaa
     8521 aggagaatac atatctgtgg ctagcagtta ttctaaggaa gatgagttaa gtttatatca
     8581 ccgtcctttt caacttactc ttaatcttat taattaccaa ctaaaaaaga agaatcattt
     8641 tgcgtcattt gtcattggtg ataatcgtac aaaagaaaca gaatccacgg gtaagatact
     8701 ttttagcgtt aaaaatatca aaccagacag cttattaata aaaatactta agcaatgttt
     8761 ttatagggct ccgattaaag gaggagattt ttccgtaagt cttacaatgc aggctataac
     8821 agcagatacg tatatgtatc tgcgggattc tgatctcatt agttttgaca atgctatctc
     8881 tgcattaatt aataacttta ataatctctg cgatttgtat ttttttcaag atgataatac
     8941 caacaataac ttcttactga tcactacaga attatttgaa cggagttttc aatatgagtt
     9001 ttcagatgaa gtttataaaa tatcaaataa ttccatggat aaaattaatc tttctgaaag
     9061 attttttgaa ttatgtcttt ggagcggagt gcgtattatc aataatagaa aacatcttat
     9121 cagcaatgaa ctttgtatct atatgggaat tactcgttca caatggtcaa tattaacaga
     9181 atggttccga aacaaccaat cactcttaaa tgcctcgctt cgttcacgtt ataatcgtat
     9241 attaagaacc tatatcactg tttgggagca atatcaagaa agcataaact ttcgtttctg
     9301 caatacagag aattcagatt tatttgaatt attttgcaaa acgcaactac aagaacttcc
     9361 atcgatgatt attgatgcaa cacagactcg cgatccttct actattgata cagccgtcga
     9421 tcttattaac cgatggcagc actcaatgaa tattgacagc cattcggtag aaaaatacag
     9481 ttacaaaggc cagcttttta acccgggttt tttcatatcc aaaaaactaa acttcaattc
     9541 agacagagag tggttcaata ttgccatcat aaatgcgttg actgacatgc gtatatgcac
     9601 atgtctttat ctgacatcaa gaatcaatac ttctgacaaa ctaatgacac attacattaa
     9661 acttatttta gagggtaaac ttattgacca aaccggtgga tatgaaactc ccactgaaga
     9721 aattgataat gcaagccaac taatcaagat attaattaga atctgcctgt ggacatggtc
     9781 tgaaaatatg gagcacaatg ggtggatgaa tagtttagca aggcgtttac gtgactatga
     9841 taaaacagac atggtgatgg gcagagttta cagtaatgtt tttgactgtg gatttattga
     9901 tatggaacaa tcatgggttc agctattatt aattttttct aaaaagaacg atagcgtatc
     9961 aaaagaaata aaagaagcaa tagaaaacaa ctatataaca tatcgtgaga agcaacgact
    10021 cattggagta ttatcaaaaa tttgtaactc catcgaatac actaaaatca agctgacttt
    10081 aacattggac gatctccaaa caaaaaaaga aaacttaaga aagttgcttc aagagcatat
    10141 taacatgcta aagaaagatc ttgatatgag acttcaggat gctgccatag atgtacatcg
    10201 actagatagc actgcacgta aaacatcaga acatcttcgt aaacgcatca agaaaacgct
    10261 ccctctttca cttttcaaat ctattgattt taagcaggct agcgattgtt tcacaaaaca
    10321 taagatatct attaagatag ataaagaacc gtatgccgaa ggaatagaat ccatccctta
    10381 tatcaatgaa ggtgatattc aagcagactt aattctcaag gacatccaga gaataatact
    10441 ttcaaattta tttagcactg gctgttcgca acataccgta atagaagatt tcaatatgtt
    10501 gattgatcac ataaaatcat ctgctgactt ggctggcaaa ctggttcttg tgatgagtaa
    10561 agagatcttt cagcaatata atcgtatgct tttcgataat cccaacctca gagaactaat
    10621 gagaaaaaat gatgatggct ctatgaacat aactactgaa tccggtaccc gtaaggttta
    10681 cttcttacca tttgttaatc aaccattttc tcttgtggta aaggataact acttcactaa
    10741 attaattatt agagaatatg ataataataa gttagtaaat gtcacttcag agaatatcaa
    10801 aagtgactca gataaattca agctaactct taactatgag ttgaatatag tatttgaagg
    10861 aaacgctgat ttaaaaatag ctcactcgca aagagtgaca tcagaataat atctttgacg
    10921 gcgctcctta gagtgccgtt cgtttattaa ctaatgctaa aaatcattat atagagaaag
    10981 tcatgtttaa atcttagaga atacaaccta gaatagtgta agcactacaa taagagaggt
    11041 cgctatgttc cgttgtccgc tttgtggcgc atctgcccgt atccgcacca gtcgtccgga
    11101 aaatgattca aacaccgtga gacaaaagta ttatcagtgt aacaatctag aatgcggcgt
    11161 atgtttctca acactggaag ccttccacaa attcacttca aagcacgcct cccccattca
    11221 ctcatca
//
LOCUS       DAJVUJ010000139        11144 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.139, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000139 DAJVUJ010000000
VERSION     DAJVUJ010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11144
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.139"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            80..256
                     /locus_tag="OQQ48_003267"
                     /pseudo
     CDS             80..256
                     /locus_tag="OQQ48_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            494..979
                     /locus_tag="OQQ48_003268"
     CDS             494..979
                     /locus_tag="OQQ48_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCS1953929.1"
                     /translation="MKNKSGNSYYREAVDIHSSLELSFILHKYHLRVDEKKSRNVFSA
                     IRDELILDGDSRQNLATFCQTWIDDEIRELLNLSIDRNMIYKDEYPQMAEPEGRCVRM
                     LSDSWSFPDSRHTLGCSTIGSSEAAMLGRLALKWQWCKKREVQGKSTEPDLRSCANML
                     A"
     gene            960..1196
                     /locus_tag="OQQ48_003269"
     CDS             960..1196
                     /locus_tag="OQQ48_003269"
                     /inference="COORDINATES: protein motif:HMM:NF012503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1953930.1"
                     /translation="MQICWHKFCRYCDVKLRKISLYDHRLIMNAEEVLKHVDENTIGV
                     VTTLGITCFYFCKADCVSVFKISVKLILVFIFIS"
     gene            1236..2696
                     /locus_tag="OQQ48_003270"
     CDS             1236..2696
                     /locus_tag="OQQ48_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCS1953931.1"
                     /translation="MSNANIAPKSKKMTVGTLAIMNITAVVSLRFIPSEAEYGLGAIF
                     YYTFAAIMFLVPVAFVAAELATTYPQKGGAFRWIGEAFGARWGFVGMLMTWLQVIPYF
                     PTVLTFGAVSVAFIDVDMGVAESIASNKWYILFFVLFVYWGAICVALRGVGIFSQVSK
                     WCGIIGTIIPAIILVILGFSYLWFSGKPAVIPLGWGDLFPNIANFQNIVLAASIFLAY
                     AGMEMNAVHVNDLDNPTKKYPIAITIASIGTIAIFLLSTLGVAFIIPTDKISLTQSLL
                     VAYDMLFEWAGVPWLGSVMAFMLAIGVLGGVVTWIAGPNTGVLAIAKAGYLPKFFQKT
                     NRHGMGHHLMFVQGIIVSVLSVTFVIMPSVQAAFQILSQLTVMLYLVMYMLMFAGAIY
                     LRHSQPDKKRPYRAPALFFWATLGFLGSFLAFVLSFIPPEQIPTGSPTSYVLYLLVLV
                     ALFLLIPILIYAFRKSDWKDPNSDFEPFSWEKRTSN"
     gene            2730..3539
                     /gene="murI"
                     /locus_tag="OQQ48_003271"
     CDS             2730..3539
                     /gene="murI"
                     /locus_tag="OQQ48_003271"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001507729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCS1953932.1"
                     /translation="MFSAESPIGVIDSGVGGLTSVKEIINLLPGEDIIYCGDNTNAPY
                     GNRSADDIISLTKKMLTFLQSRNVKLVAVACNTISSTLESEEYSGYAKSFPFPILSII
                     EPAVEDVIRQQYKNVGIIATEFTIKTGCHKELIKKLNPTINIFGEPSKNLAMLIEEGN
                     LNAPAILNDIKKHVNHLISLHPVNEIILGCTHYPIVQSLFEAVAPDIKFINPAHDQAI
                     SIKNHLGQLNLLNSSNIGTLQINTSGSMEIYKTVLGELSITKPHTFSIRQF"
     gene            complement(3658..4370)
                     /locus_tag="OQQ48_003272"
     CDS             complement(join(3658..4106,4106..4370))
                     /locus_tag="OQQ48_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCS1953933.1"
                     /translation="MARVNIHCPRCQSAQVYRHGQNPKGRDRLRCRDRLRCRDCHCVF
                     QLTYTYQARKPGMKELITEMAFNGAGVRDTARTLKIGSNTVIRTFKKLAPKRITSSPV
                     THADVAFICELDEQWSYVGSKARQHWIWYAYNTKTGGVLAYTFGPRTDQTCRELLALL
                     TPFNIGMLTSDDWGSYGREVPKNKHLTGKIFTQCIERNNLTLRTRIKRLGRKTICFSR
                     SVEIHEKVIGAFIEKHMFY"
     gene            4896..5885
                     /gene="umuC"
                     /locus_tag="OQQ48_003273"
                     /pseudo
     CDS             4896..5885
                     /gene="umuC"
                     /locus_tag="OQQ48_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744069.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V subunit
                     UmuC"
     gene            6089..6715
                     /locus_tag="OQQ48_003274"
     CDS             6089..6715
                     /locus_tag="OQQ48_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010260829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family plasmid-partitioning AAA ATPase"
                     /protein_id="HCS1953934.1"
                     /translation="MIIVLGSQKGGVGKSTLAVSIAAYLMSLGNRVIIVDADDQKSVL
                     TWYNNRPEDLPHIPVTGATGNIKAMLKEHEKSYDFVIADCAGRDSAEMRSGLMAADVF
                     ISPLRPSQMDLDVVPHTCSVFTAAKDFNEDVRGYLVLNMTPTNMFVNEANEAAEVLKD
                     YPEMHLANSRVCDRKAHRDAWAESMTIFETQNDKAQQEIEALVKEVIL"
     gene            6712..7014
                     /locus_tag="OQQ48_003275"
     CDS             6712..7014
                     /locus_tag="OQQ48_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1953935.1"
                     /translation="MRNRANTSPRDEAAFINGGTAGLDNTKKSSDKSSVVSKAKPVSI
                     SFFEENLKDIDALITAEMVSGKGRVNRSDVVRAAVEALKTLSKTEVSRLIAEVKQR"
     gene            complement(7481..9058)
                     /locus_tag="OQQ48_003276"
     CDS             complement(7481..9058)
                     /locus_tag="OQQ48_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing methyl-accepting
                     chemotaxis protein"
                     /protein_id="HCS1953936.1"
                     /translation="MRQNLPVTGRNLELPKDANILSTTSPQSHITYVNPDFIKISGFT
                     EEELLGQPHNIVRHPDMPPAAFEHMWSTLKSGRSWMGLVKNRCKNGDHYWVSAYVTPI
                     AKNGSIVEYQSVRTKPEPEQVLAAEKLYAQLRSGKAARPKLAASFSVKILLLIWGSII
                     SSAMAAGMLTDTSISSLLLATLMSGSLSSVSVLAILSPLGRLVERARNISNNPLSQSL
                     YTGRTDEFGQIEFALRMMQAETGAIVGRIGDASNRLSEHTRGLLKDIESSNVLTVEQQ
                     AETDQIATAVNQMVASIQEVASNAQHAADAAGRADTETASGQRLVAHTSQSITALEGE
                     IRQATQVIHELEGQSNEISKVLDVIRGIAEQTNLLALNAAIEAARAGEQGRGFAVVAD
                     EVRSLAARTQQSTTDIQSMISALQERAQSAVTVMEQSSRQAHTSVAHAEEAATALDGI
                     GQRVNEITDMNAQIATAVEQQGAVSEDINRSIINIRDAADTNVQTGQNNLQSAKSVAQ
                     LTSALSELAKQFWEKRG"
     gene            9368..9928
                     /locus_tag="OQQ48_003277"
     CDS             9368..9928
                     /locus_tag="OQQ48_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCS1953937.1"
                     /translation="MQGHRIGYVRVSSFDQNPERQLEQTQVSKVFTDKASGKDTQRPQ
                     LEALLSFVREGDTVVVHSMDRLARNLDDLRRLVQKLTQRGVRIEFLKEGLVFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKALSDEQAATLRQRATA
                     GEPKAQLAREFNISRETLYQYLRTDD"
     gene            9932..>11144
                     /locus_tag="OQQ48_003278"
     CDS             9932..>11144
                     /locus_tag="OQQ48_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs1 family transposase"
                     /protein_id="HCS1953938.1"
                     /translation="MPRRLILSATERDTLLALPESQDDLIRYYTFNDSDLSLIRQRRG
                     DANRLGFAVQLCLLRYPGYALGTDSELPEPVILWVAKQVQAEPASWAKYGERDVTRRE
                     HAQELRTYLQLAPFGLSDFRALVRELTELAQQTDKGLLLAGQALESLRQKRRILPALS
                     VIDRACSEAIARANRRVYRALVEPLTDSHRAKLDELLKLKAGSSITWLTWLRQAPLKP
                     NSRHMLEHIERLKTFQLVDLPEGLGRHIHQNRLLKLAREGGQMTPKDLGKFEPQRRYA
                     TLAAVVLESTATVIDELVDLHDRILVKLFSGAKHKHQQQFQKQGKAINDKVRLYSRIG
                     QALLEAKESGSDPYAAIEAVIPWDEFTESVSEAELLARPEGFDHLHLVGENFATLRRY
                     TPALLEVLELRA"
BASE COUNT         2966 a         2527 c         2597 g         3054 t
ORIGIN      
        1 gtgggttacc cccaaacgaa tcggaaaatc gatactggaa aaactctaac gcggtggcca
       61 gtttttgttg accacttcac ttccgaaaga aaagcacctg accggcaaga tttttactca
      121 gcgcattgag cgcaataacc tgacgctgag aaccagaatc aaacggctgg cccgcaaaac
      181 gctctgtttt tcacgttcta ttgagcttca tgaaaaagtc atcggggcat tcatggaaaa
      241 atgcatgttc tactaattgg atgcatcacc gttgaaatca caataacgat ttttttgtaa
      301 atatgctctc acatatgtag gaatagacca ggttggcatt gaaacacatc tatcatgacc
      361 ataataaagt aattttttga tatgtttata gttttatatt aattaaaaca tatctaaaat
      421 tgatagtttt aaaattgcaa aatccatatt ttgtttgtaa tgtttaatct gattatattg
      481 agtggcgttt attatgaaaa ataaatcagg taattcttat tatagagaag cggtcgatat
      541 tcatagctca cttgagcttt catttatatt gcataaatat catttgcgtg tagatgagaa
      601 gaaatctcga aatgtattca gcgccatacg tgatgagctc atccttgatg gggattcacg
      661 acaaaactta gctactttct gtcaaacatg gattgatgat gaaatccgtg agttgctaaa
      721 tttatctatt gatagaaaca tgatttataa agatgaatat ccacaaatgg cggaacctga
      781 aggtcgctgt gtgcgtatgc tttccgattc gtggagtttc cccgattcgc gacacacctt
      841 aggttgctca accattggtt catctgaagc tgctatgctc ggccgcttgg cgttaaaatg
      901 gcagtggtgt aaaaaacgcg aagtacaagg taaatcaacc gaacctgatt tgcggtcctg
      961 tgcaaatatg ctggcataaa ttctgccgtt actgtgatgt gaaattgcgt aaaatttcac
     1021 tttatgatca tcgcctgata atgaatgctg aagaggtttt gaagcatgtc gatgaaaaca
     1081 ctattggtgt cgttacgact ttaggtatta cttgttttta tttttgtaaa gcagattgcg
     1141 ttagtgtttt taagataagt gtcaagttaa tattagtttt tatatttata tcgtaattat
     1201 atgtttctat tttaaaaata ttcgagagta taaaaatgtc gaatgctaat atcgcaccca
     1261 aaagtaagaa aatgacagtg gggacactcg ctatcatgaa tataactgct gtagtaagtc
     1321 tacgatttat accttctgaa gcagaatacg gcttaggtgc cattttttac tatacctttg
     1381 ctgcaattat gtttttagta cctgttgcct ttgttgcggc tgagctagct acaacatatc
     1441 cacaaaaagg gggggcattt cgatggatag gagaagcatt tggggctcgc tggggttttg
     1501 taggaatgct tatgacctgg ctacaagtca tcccctactt tccaacagta ctgacatttg
     1561 gtgcagtctc ggttgcattt attgatgttg atatgggggt ggcagaatca attgccagta
     1621 ataaatggta tattttattt ttcgtactat ttgtttactg gggagctatt tgtgttgcat
     1681 tacgtggcgt tggtattttc tcgcaggtat ctaagtggtg cggtattata gggacaatta
     1741 ttccagctat tatccttgtt attcttggtt tctcctatct gtggttcagc ggtaaaccag
     1801 cggtgatccc attaggttgg ggtgatcttt tccctaatat agctaacttc cagaatattg
     1861 ttcttgccgc cagcatattt cttgcttatg cagggatgga aatgaatgcg gtccatgtta
     1921 acgatttaga taatccaact aaaaaatacc caattgcaat taccatcgct tctataggta
     1981 ctattgcaat attcctgctc agtactttag gggtcgcctt tattattcct acagataaaa
     2041 ttagtctaac tcaaagtttg ttggttgcct acgatatgct gttcgaatgg gctggagtgc
     2101 catggcttgg ctctgtgatg gcatttatgc tagcaattgg cgtactgggt ggggtggtta
     2161 cttggattgc agggcctaat acaggtgtat tagcgatcgc gaaagctggt tatttaccta
     2221 agtttttcca aaaaaccaat cgtcatggaa tggggcacca tctgatgttt gtacaaggta
     2281 taattgtctc agttctttct gttacttttg tgataatgcc ttcagttcaa gcagcttttc
     2341 agattttatc acagttaaca gttatgctct atttagttat gtatatgctc atgttcgctg
     2401 gtgctatcta tctacgacat tctcaacctg ataaaaaacg cccatatcga gcccctgctt
     2461 tattcttctg ggcaacactt ggattcttag ggtcttttct ggcttttgta ttaagtttta
     2521 ttccgcccga gcaaatccca actggtagtc caaccagcta tgttctttat ttgttagtat
     2581 tagttgccct atttttatta attccaatat taatttacgc cttccgaaaa tctgactgga
     2641 aagatccaaa ctctgatttc gaaccatttt cttgggaaaa aagaactagt aattaaattt
     2701 tcaataagct acaaaagatg aggttaaata tgtttagtgc tgaatctcca attggtgtaa
     2761 tagactcagg tgtaggtgga ttgacatcgg taaaggaaat aattaatttg cttcctggcg
     2821 aagatattat ctattgtgga gataatacga atgcacctta tggcaaccgt tctgccgatg
     2881 atatcatcag tttgactaaa aaaatgctga cttttttaca gtcaaggaat gtcaaactag
     2941 ttgctgttgc atgtaatact atatcctcaa ctttggaatc cgaagaatac agcggatatg
     3001 ctaagagctt tccgtttcct atcttatcca tcattgaacc tgcagtagaa gatgtgattc
     3061 gccagcagta taaaaatgtg ggtattatcg ctaccgagtt tactattaaa acggggtgtc
     3121 acaaagagct aataaaaaaa ttgaacccaa caataaatat atttggagaa ccaagcaaaa
     3181 atcttgcaat gttaattgag gaaggtaatc ttaatgctcc tgcaatattg aatgatatca
     3241 aaaaacatgt taaccattta atttccctcc atccagtaaa tgaaattatt ctcggctgta
     3301 ctcactatcc aattgttcag agtttatttg aagcagttgc accggatata aaatttatta
     3361 atcctgcaca tgatcaggct atctctatta aaaatcactt gggacagcta aatttattaa
     3421 actcctcaaa tataggaact ttgcaaataa atactagtgg aagtatggag atctacaaaa
     3481 cggtactagg ggaattgagc attacgaaac ctcatacatt cagtatcagg cagttttaaa
     3541 tttatccggt gcaccgtaaa accccgtcct tctgaactgc accccaaaag ttggacacca
     3601 actgagtaag gtgcatttta tgggcaaacc cagttacaca ctgggtagtg catccaatta
     3661 gtagaacatg tgtttttcga taaacgctcc gatcactttt tcgtggatct caactgagcg
     3721 tgagaagcag attgttttac gacccaaccg cttaatgcgg gtgcgtagcg tcagattatt
     3781 acgctcaatg cattgggtga atattttgcc ggtcagatgc ttattcttcg gcacctcccg
     3841 gccatagctg ccccagtcat cgctggtcag catgccgatg ttgaagggtg taagcagtgc
     3901 cagtagctcc cggcacgttt gatcggttcg gggaccaaaa gtgtaggcca gtacaccgcc
     3961 tgttttggtg ttgtacgcgt accagatcca gtgttgccgg gctttactgc caacgtagct
     4021 ccattgctca tcaagctcgc agataaacgc cacatcagca tgggtaacag gcgaagaagt
     4081 tattcgcttt ggcgcgagtt ttttaaagtc cggatgacgg tgttactacc aattttcagt
     4141 gtcctggcgg tatcgcgaac cccggcacca ttaaaggcca tttcagtgat cagctctttc
     4201 atacccggct tacgtgcttg ataagtataa gtgagctgaa acacacagtg gcagtcacgg
     4261 cagcgaagtc tgtcacggca gcgaagtctg tcacggcctt tagggttctg accatggcgg
     4321 taaacctgag ctgactgaca acggggacaa tgaatattaa ctctggccat gagataacct
     4381 caaaaacccg tattatacat cagattcaac taattagagg catcacctat ccagcattat
     4441 ataacatact aacaataact aaattataat aatcacaact tataatcaaa taataaacgc
     4501 ataaaatcaa caatgatttt ttctggttat atttatctaa aaaaacggaa tattatgtaa
     4561 cttattattt tctaagcgta aatttattat gtggtcagac taacaaactt gcgtaccacc
     4621 cgaagaatat gcgaaaattt aagtattctt tcttttttta ttttttcatt ttctcttttc
     4681 ttctctttcc ggttctcttc ttttctggtt ttggtttttt cctttttatt tttattcttt
     4741 cttcagggtt gtgctaaaac accaaaacat ttttcaccac aaaagcatcc aatcatcttg
     4801 ccagaccaat caccttaacc ggatacactg ccggatataa tcccactaac acccagccac
     4861 cccgcaccat tcctgataac taccgtattc aggaagttga gccctactca attgatgaaa
     4921 tgttcattga tttgcggggg ataaatcatt gcatctctcc ggaggctttt ggtcatcagc
     4981 tcagggaaca ggtaaagagc tggacaggac tcaccatggg ggtgggcatt gcgcctacaa
     5041 aaacgctggc taaaagtgca cagtgggcaa caaagcaatg gccacagttt tccggtgtgg
     5101 tcgcgctgac ggcagaaaac cgtaattgga tcttgaagct actggggctg cagccagttg
     5161 gtgaggtctg gggagtagga cgcagactga cggaaaagct gaatgcgctg ggtattaaca
     5221 cagcactgca actggcgcag gctaacacgg cattcatccg gaaaaacttc agcgtcattc
     5281 ttgagcgtac ggtacgcgaa ctcaacggcg agtccagcat atccctggaa gaagcaccac
     5341 cggcaaaaca gcagattgtc tgtagtcgca gttttggtga acgaatcaca gacaaagatg
     5401 ccatgcacca ggctgttgtt cagtatgcag agcgggccgc agagaaacta cgtggggagc
     5461 gtcagtattg ccggcaggtg acgacatttg tacggacatc cccctttgca gtaaaagaac
     5521 cctgttacag caatgccgct gtggaaaagc ttccattgcc cacacaggac agccgggaca
     5581 ttattgccgc agcatgcaga gcgttaaacc atgtctggcg tgaagggtac cgctatatga
     5641 aggcaggtgt catgctggct gatttcacac catcgggtat agcgcagccg ggattatttg
     5701 atgaaatcca gccccgtaaa aacagtgaaa agttaatgaa aacactcgat gaactgaacc
     5761 agtcgggaaa agggaaagtg tggtttgcgg ggcgaggaac cgcccctgaa tggcaaatga
     5821 aacgggaaat gctttctcct gcgtatacca ccagatggac agatttgcca gtagcccagt
     5881 tatgaatatg taatccaccc ataatgttgt tatcttgtta tcttgttatc ttgttatctt
     5941 gttatcttgt tatcttgtta tcttaattaa tctataaatc aaatacttaa caacttgcta
     6001 agttgttaac ataacaaaaa taattgcatg attcgcgttc agatggcaat attaagttat
     6061 taacttaaca gcttaatatg aggctataat gataatagta ttaggtagtc aaaagggtgg
     6121 ggtaggtaaa agtacactgg cggtatctat agctgcgtat ctgatgtcac taggcaatag
     6181 agtgattatt gtcgacgcag atgaccagaa atcagtatta acctggtaca acaatcgccc
     6241 tgaagatctc cctcatattc cggttacagg cgcgacaggt aacatcaaag ccatgttaaa
     6301 ggaacacgaa aaatcctatg attttgttat tgcagactgt gctggccgtg atagcgcaga
     6361 aatgcgtagt ggattgatgg ccgcagatgt attcatttct cctttgcgcc catctcagat
     6421 ggacctggat gttgtgcctc atacatgctc cgtttttaca gcagcaaaag attttaatga
     6481 ggatgttcgg ggatatctcg ttttaaacat gacgcccaca aatatgtttg ttaatgaagc
     6541 caatgaggca gctgaagtac ttaaggatta ccctgaaatg catctggcaa actcccgggt
     6601 atgtgatcgt aaagcacatc gagatgcttg ggccgaatca atgacaattt ttgaaacgca
     6661 aaatgataaa gcccagcaag aaatcgaagc gttagtgaaa gaggttattc tgtgagaaat
     6721 agagcaaaca cttcacctcg tgacgaggca gcatttatta acggtggcac ggctggcctt
     6781 gataatacca aaaagtcatc agataaatca tcggttgtaa gcaaagcaaa accagtgagt
     6841 atttcttttt ttgaagaaaa cctaaaagac attgatgccc ttataacggc tgagatggtt
     6901 tcaggtaaag gcagagtgaa tcgttctgat gttgtcaggg cggcggtaga agcacttaaa
     6961 acactatcaa aaacagaggt atcacgattg atagcagaag taaaacaaag ataacacctt
     7021 gagataacaa gataacacct tgttatcttg ttatcttgtt atcttgttat cttgttatct
     7081 tgttatcttg ttatcttgtt atcttgttat cttcaatgta ttttaggatg acctatgttg
     7141 aatagaacct aaaacaaaac aaatattaat aggggtccgc ttggaaaagg gaaattttcc
     7201 cacgctaaga gaattgagtt gaatgagtgg ttacatccag gccagctaac tcactgaacc
     7261 ccgtacaggg tcagacattc ggtatctcct tgggggaacc gcagaattcg gaaaaaatcg
     7321 tacgctaagc taacggtgtt ctcgtgacag ctctttgact aggctttcta aggccattct
     7381 gatagccctg acttcctgaa aagccatggc taaaatttgt gcggctaaaa gggataaccg
     7441 atggtaaagt aagttatccc tgtcgagata ctgaaaagcg ttatcctcgt ttttcccaaa
     7501 actgttttgc cagttcgctc agagcgctag ttaactgagc gacagatttc gcactttgca
     7561 aattattctg cccggtctgt acattggtat cagcagcatc gcgtatattg ataatactgc
     7621 ggtttatgtc ttcacttact gctccctgct gctcgaccgc agtcgctatt tgcgcgttca
     7681 tgtcggtaat ttcgttaacg cgttggccaa ttccatcaag agctgtagct gcttcctctg
     7741 cgtgagctac actcgtgtgc gcttgccgac tactttgctc catgactgta acagcggatt
     7801 gcgctcgctc ttgtagagcg ctgatcatgc tttgaatatc cgttgtcgat tgctgtgtgc
     7861 gagcagcaag actgcgaacc tcatcggcga caacagcaaa accacgcccc tgctcaccag
     7921 cacgcgcggc ctcaattgct gcgttgagtg ccaacaaatt cgtttgctcg gcgatccctc
     7981 gtataacgtc aagaactttt gatatctcgt tactttgacc ttcaagctca tgaataacct
     8041 gagtggcttg cctaatttca ccttcaaggg cagtgattga ctggcttgtg tgggctacca
     8101 gacgctggcc agatgccgtc tcagtgtctg ctcttccggc cgcatctgca gcatgctgtg
     8161 cattgctcgc aacctcttga atgcttgcca ccatttggtt tactgccgtt gctatttgat
     8221 ctgtctctgc ctgctgctca actgtaagta cattgcttga ctcaatatcc tttagtaggc
     8281 ctcgggtgtg ttcgctaagc cgatttgatg catcacctat gcgacctact atggcgcctg
     8341 tttcagcttg catcattcgt aaagcaaact ctatttggcc aaactcatcg gtgcgcccag
     8401 tgtagaggga ttgacttaat gggttattgg aaatattcct ggctctttca accagtcttc
     8461 caagaggaga gagaatagcc aaaacactaa cagagcttaa gcttcctgac attaaagtgg
     8521 ctaacaataa gctgcttatt gatgtatcag taagcatgcc ggcagccatt gcgcttgata
     8581 taatactacc ccatatgagc aagagtattt tcacggaaaa gctagcagcc aatttcggcc
     8641 tcgcggcctt cccgcttctc aattgagcat ataatttttc cgcagccaaa acctgctcag
     8701 gttcaggctt ggtccttaca gactggtatt caacaatcga accattctta gctattggcg
     8761 ttacataagc acttacccaa tagtggtcgc catttttaca gcgatttttt actagcccca
     8821 tccatgagcg gccagatttt aatgtactcc acatatgctc aaatgcagca ggcggcatat
     8881 ctgggtgtct tacgatgttg tgaggctggc ctaatagttc ttcctcagtg aaaccactga
     8941 ttttaatgaa gtcaggatta acgtacgtga tatggctttg aggggaggta gtcgaaagaa
     9001 tattggcatc ttttgggagt tctaagtttc gacccgtcac tggtaaattc tggcgcataa
     9061 gaacctcaag ggttggctgt tttattttat tgttttcggc attaagccca atttcttgag
     9121 cgttacgata aagctagcat ggaaacgata ggtgcaagca agttaagggt tgcatcgcgc
     9181 atgtcaatct aggctatacc ctaacttgat gtcaggcagg gccgcgccgc ttcgtcagaa
     9241 tagagtctgc tttcccattt tttgacacat gcccgcgaag gttatagatt tcagcctgac
     9301 agaaatgggc tttgaggcac aacggaacag aaagtgcact taagccgcct tcaaccaagg
     9361 agacatcgtg caggggcacc gcatcggcta cgtccgggtc agcagctttg accagaaccc
     9421 ggaacgccag ctggaacaaa cccaggtgag caaggtgttc accgacaagg catcgggcaa
     9481 ggacacccag cgcccccagc tcgaagcgct gctgagcttc gtccgcgaag gcgatacagt
     9541 ggtggtgcac agcatggatc ggctggcccg caacctcgat gacctgcgtc gcttggtaca
     9601 gaagctgact cagcgcggcg tgcgcatcga gttcctgaag gagggcctgg tgttcactgg
     9661 cgaggactcg ccgatggcca acctgatgct gtcggtgatg ggggccttcg ctgagttcga
     9721 gcgcgccctg atccgcgagc ggcagcgtga gggcatcgcc ttggccaagc agcgtggcgc
     9781 gtaccggggc cgcaagaaag ccctgtccga tgagcaggct gctaccctgc ggcagcgagc
     9841 gacggccggc gagcccaagg cgcagcttgc ccgcgagttc aacatcagcc gggaaaccct
     9901 ctaccagtac ctccgcacgg acgactgaca catgccgcgt cgcttgatcc tctcggccac
     9961 ggagcgggac accctgcttg cgctgccgga aagccaggat gacctgatcc gctactacac
    10021 cttcaacgac tccgacctgt cgctgatccg ccagcgacgc ggcgacgcca accgcctcgg
    10081 cttcgccgtg cagctctgcc tgctgcgcta ccccggttac gcgctgggaa ccgacagcga
    10141 gctgcccgag ccggtcatcc tgtgggtggc gaagcaagtc caggccgagc cggcgagctg
    10201 ggcaaagtac ggcgagcgcg acgtgacccg tcgcgagcat gcccaggaac tgcgcaccta
    10261 cctgcaactg gccccgttcg gcctgtccga cttccgcgcc ctggtgcgcg agctaaccga
    10321 gctggcccag cagaccgaca aaggcttgct gctggccggt caggccctgg agagcctacg
    10381 gcagaaacga cgcatcctgc cggcgctgag cgtgattgac cgggcctgct cggaagccat
    10441 tgcgcgagcc aatcggcggg tctaccgcgc cctggtcgaa ccactcacgg actcgcatcg
    10501 ggccaagctg gacgagctgt tgaagctcaa ggccggcagc agcatcacct ggttgacctg
    10561 gctgcgccag gcaccgctga aacccaactc tcggcacatg ctggaacaca tcgagcggct
    10621 gaagacattt cagttggtgg acttgcccga aggcctgggc cggcacatcc accagaaccg
    10681 cctgctcaag ctggcccgcg agggtgggca gatgacgccc aaagacctcg gtaagttcga
    10741 gccgcagcgc cgctacgcga ccctggccgc cgtggtgctg gagagcaccg cgaccgtgat
    10801 cgatgagctg gtcgatctgc atgaccgcat cctggtcaag ctgttcagcg gcgcgaagca
    10861 caagcatcag cagcagttcc agaagcaggg caaggcgatc aacgacaagg tgcgcctgta
    10921 ctccaggatc ggccaggcgc tgctggaagc gaaggaaagc ggcagcgacc cctatgccgc
    10981 catcgaggcg gtgattccct gggacgagtt caccgagagc gtcagcgagg ccgagctgct
    11041 ggcccggccg gaaggcttcg accacctgca cctggtcggc gagaacttcg ccaccctgcg
    11101 ccgttacacg ccggccttgc tggaggtgct ggaactgcgc gccg
//
LOCUS       DAJVUJ010000140        11094 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.140, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000140 DAJVUJ010000000
VERSION     DAJVUJ010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11094
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.140"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            353..1711
                     /locus_tag="OQQ48_003279"
     CDS             353..1711
                     /locus_tag="OQQ48_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HCS1953939.1"
                     /translation="MKRKIIPVLIGCTLSFSALAAQPTAERYVVSFPEGTHVNYAGAF
                     ASAFPNGLPVGIGSGLLFTGKQGDALTFATITDRGPNADSPKEGKNETKIFVTPDFAP
                     LLMTIRVQNGKAEAIDPRPLHDDKGAINGLPLASDVIGSTNEVAFSDTLHRLKGDNHG
                     LDTEGITPDGKGGYWLCDEYGPFLINIDSKGKILAIHGPQAAEGEKAIAGGLPNILKW
                     RQANRGFEGLTRMPDGRIIVAVQSTLDIDAKSKKKALFTRLVSFDPASGKTAMYGYPI
                     DSAAYSKNSDAKIGDIVALDNQHILLIEQGRDKNNRMRNLIYEVDLNKASDLSGFDKP
                     GEYPEFDDEKTLSQRGITLAQKTQVVDLRSLGWQQEKAEGLALIDSKTLAVANDNDFG
                     VKVAMQHPVEGKTFKDYRVNAEGKLTLDDKQVETTLRVKPLEKPESDSELWIVTLPEA
                     LK"
     gene            complement(1950..3335)
                     /locus_tag="OQQ48_003280"
                     /pseudo
     CDS             complement(1950..3335)
                     /locus_tag="OQQ48_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(3385..3732)
                     /gene="tnpB"
                     /locus_tag="OQQ48_003281"
     CDS             complement(3385..3732)
                     /gene="tnpB"
                     /locus_tag="OQQ48_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCS1953940.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNGFNGLAAKAQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            complement(3729..4109)
                     /locus_tag="OQQ48_003282"
     CDS             complement(3729..4109)
                     /locus_tag="OQQ48_003282"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HCS1953941.1"
                     /translation="MQKNVTPGRRKGCPNYPPEFKQQLVAATCEPGISISKLALENGI
                     NANLLFKWRQQWREGKLLLPSSESPQLLPVTLDAAAEQPESLAEDPETLSISCEVTFR
                     HGTLRFNGNVSEKLLTLLIQELKR"
     gene            complement(4464..4898)
                     /locus_tag="OQQ48_003283"
     CDS             complement(4464..4898)
                     /locus_tag="OQQ48_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HCS1953942.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDENYEGEKCCPRCGTGVYSRAWGRQEVPSEDARADNPLQRL"
     gene            complement(4886..5287)
                     /locus_tag="OQQ48_003284"
     CDS             complement(4886..5287)
                     /locus_tag="OQQ48_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1953943.1"
                     /translation="MYAKSFIALDGNGRLTGARTAQAAPYANYTCHLCGSALRYHPQY
                     DTELPWFEHTDDRLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGGFSIPRTTQEEICEF"
     gene            complement(5547..6116)
                     /locus_tag="OQQ48_003285"
     CDS             complement(5547..6116)
                     /locus_tag="OQQ48_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HCS1953944.1"
                     /translation="MTYKYNPFWQQRIRETVRHALDVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPTTLHYVWAREFGECKGKKHYHLMLLVN
                     RDTWCRAGDYRAPESLAGMIKQAWCSALGVDVGCHATLVHFPAWPAVWLARNDDTGFQ
                     QVLERADYLAKEHTKAHCTGERNFGCSRS"
     gene            complement(6789..6998)
                     /gene="hha"
                     /locus_tag="OQQ48_003286"
     CDS             complement(6789..6998)
                     /gene="hha"
                     /locus_tag="OQQ48_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCS1953945.1"
                     /translation="MEVKTKEDWLYQFRRCSSRETLEKVISHTRYKLTPAELAFNSAV
                     DHRLAELTMNKLYDRVPASVWKYVT"
     gene            7697..7903
                     /locus_tag="OQQ48_003287"
     CDS             7697..7903
                     /locus_tag="OQQ48_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCS1953946.1"
                     /translation="MNTPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKDGAFPAPIKL
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            7989..8561
                     /locus_tag="OQQ48_003288"
     CDS             7989..8561
                     /locus_tag="OQQ48_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1953947.1"
                     /translation="MQKLENRCDLLLTQHQKWMASVTRFIVAHGMGSPHLHGYHRLTL
                     AHFFLPEKGTIISVAPQGLYQVVNPATPPFIPAIQEGLMTSIQTHEIMLLTHFNLGGV
                     LLSELHRLGESRLANRLNSLLRRFDDRDLYHTLIWLCWYDLMCAHSMQPWTEELKHKS
                     HAELESWAVARKREKRELELMIDEYLLYAC"
     gene            8770..8877
                     /locus_tag="OQQ48_003289"
                     /pseudo
     CDS             8770..8877
                     /locus_tag="OQQ48_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            10054..11073
                     /locus_tag="OQQ48_003290"
     CDS             10054..11073
                     /locus_tag="OQQ48_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCS1953948.1"
                     /translation="MNNEQMLIKCKDPRKCRFYAEYLNHKYPNEFPQFAPDNPQNCIR
                     NVNSFLKEKYYKASNNSFGVFGSTAEEALSKEINELEIIAGQAILPEESFLWLKTDEL
                     ATFFTWTTIYLSKEDHTKFPKINHNSSGIKNGIEVRKIRTVTDETLNTYKKSNLPDNP
                     SSHNERIKVIISYFDSCHSLINKNAKSIYLEELRQAWLKKKSACQKFKWLLPDDDEMC
                     QWFWTRLKQKQDEKHPVHNPVTRWFIPSSTKEYYLASLVAFALWKEAPDTIELFRTRI
                     NHAWHQKKQKDKYRSDGKKAINIHIRSNVKEMLDELRTVYGLRTGEFFEMLITEKYLE
                     HKRRK"
BASE COUNT         2925 a         2828 c         2614 g         2727 t
ORIGIN      
        1 gggatatggc cctgcctttc cagcaatcgt cgattgttat accagtccac ccacgtgagt
       61 gtggtcagct catcatactg acacgtgcat tacccctacc gcgataatgc aaccgtacaa
      121 tatagggcgt ttcctaaacg cagccctcat cactccaatc caacaagaca cagcattttt
      181 gatcacgaag aaagacaaat aatttccgta aatgtttccg ttgttacaaa taacaacagt
      241 gttttacatt tctttggcct caggcccgcg catgttgcca ttcgatgtta ttttcctgac
      301 tcgtttcttc tttatggtgt agtgtgatct tcactttatg gatgctaaca atatgaaaag
      361 aaaaattatt cccgtgctaa tcggttgtac gctctctttc tctgccctgg cggcacaacc
      421 tacggctgag cgctatgtcg tcagttttcc agaaggcacg cacgtgaact acgccggcgc
      481 atttgccagt gcattcccga acggccttcc ggttgggata ggttctggcc tgctgttcac
      541 cggtaaacag ggcgatgccc tgacattcgc aaccattacc gatcgcggtc cgaacgcgga
      601 ttcaccgaaa gaggggaaaa acgaaactaa aattttcgtt acgccagatt tcgcgccgct
      661 gctgatgacg attcgtgtac aaaacggcaa agcggaggcc atcgatcccc gtcctctgca
      721 tgacgataaa ggtgcgatca acggtctgcc gctggcaagc gacgtgattg gctccaccaa
      781 tgaagtggcc ttcagcgaca cgttacatag actgaagggg gataatcacg ggctggatac
      841 ggaagggatc actccggatg gtaaaggagg ttactggctg tgcgatgagt atggcccgtt
      901 cctgattaac atcgacagta aagggaaaat cctggcaatc cacggtccgc aggcggcgga
      961 aggggaaaaa gcgattgcgg gcggtttgcc aaatatcctc aaatggcgtc aggcgaaccg
     1021 cggctttgaa ggcctcaccc gtatgccgga cgggcgtatt attgtcgccg tgcaaagtac
     1081 gctggatatc gacgctaaga gcaagaaaaa ggcgctgttt acgcgtctgg tgagcttcga
     1141 tccggcaagc ggaaaaaccg cgatgtacgg ttatcctatc gacagcgcgg cctacagcaa
     1201 aaacagcgac gctaaaatcg gagacatcgt ggcgctcgat aaccagcaca tcctgctgat
     1261 cgaacaggga agagataaaa acaacagaat gcgtaacctg atttacgagg tcgatctcaa
     1321 taaggcgagc gacctgtcag gcttcgacaa gccgggtgag tatccggagt ttgatgatga
     1381 gaaaaccctg tcacagcgcg gcattaccct tgcgcagaaa acgcaggttg ttgatctgcg
     1441 ctccttgggc tggcagcagg agaaggctga agggttggcg ctgatcgaca gcaaaacgct
     1501 ggcggtggcg aacgataacg actttggcgt gaaggtggcg atgcaacatc cggtcgaggg
     1561 caagacattc aaggattatc gggtgaacgc ggaaggaaag ctgacgcttg acgacaagca
     1621 ggttgaaacg accctcagag tgaagccact ggagaaaccg gagtctgaca gtgaactgtg
     1681 gattgtgacg ctgccggaag cgttgaaata agtttataag gtacggacag cagtatcgct
     1741 tagtttttca aattcatgta tgtgaacaat attggggttc cggcgtagac cctcaatgaa
     1801 agcgtcacca cgtgaaaaca tgcctgcacg gtttacacgt catcccatgg tggcagccgg
     1861 ttaactttcc tgctacacca gtggaaaaag gccataagca gtccgaacaa aagtaagcgt
     1921 acagcgaggg ccgtattgac ggggatgtgt tattcagctg gcagtgctat gcgccacgga
     1981 agcagttcgc tgacccggtt gaccggccag tctgctatga cgccaagcac atggcgaagg
     2041 tagctttctg gatccacgtc attcagtttg cacgtcccga tcaggctgta cagtagcgct
     2101 ccccgctcac caccatggcc agagccgaag aacagggtct tcatcttttc acgcaaccag
     2161 ctttccaggg atttcaacag cggtttcgtt tttcgctgac gttcagcaag ccgctgctct
     2221 gccggcattc cccttatatc cgcctctatg gcgtacaact gaccgatctg ctccagggct
     2281 tcttccgtca gtgctgacgg gatgcggacg tgcacatcgt ggatctttca gcgggcatga
     2341 gcccagcagg cagcttccgt tatcccacca ttgcgataca gctcgttgaa cccggcgtac
     2401 gcatccgctt gcagcacacc gctgaagcaa gcaagatgag tctgcggatg gatgcctttt
     2461 ctgtccgggc tgtaagcgaa cctcactgca ggtgccaacg ctgacccggc attgcggtca
     2521 tcacgaacat acgcccacaa ccgcccggtc ttcgtcttct tattacccgg cagcagtacc
     2581 tggaccgggg tatcatcggc atggagtttg ccgtcagtca tgacatagcc atgaagcgcc
     2641 tcttccagcg gagacagcag ccggcagcat gcatccaccc agcccgacag cagtgaacgg
     2701 ctcagctcca caccttgccg gccgtatatt tctgactggc gatacagcgg ggtgtgctct
     2761 gcatacttcg aggtcagcac gcgggccagc agccccggtc cggcgatacc ccgctcgatg
     2821 ggccgcgaag gtgcaggtgc ctgcacgatg gtatcgcact gagtacaggc atgtttttcc
     2881 tgtaccgtcc ggataacccg gaaggcgcta cgcatcaact ccagctgttc ggcggtatcc
     2941 tcgcccagat agctcagtga accgccgcag ttcgggcagc acggcgccgc aggcaacagt
     3001 cgcttttcgt cacggggtag tgattcaggg aacggcttac gggtgcgggt ctgacgcaac
     3061 ggacgctgta ctgccgggtc atacacccta ccagtcagcg tatcgctctc tttctgaagt
     3121 cggttcagat cggcttccat ttgtgcgata cggcgggaga ctttttcgga acgactgccg
     3181 aagttcatcc ggcggagttt atccagctgc gcctgcagat ggtctatttc gcgctcccgg
     3241 ttgctcagct tttcctgcag ggcgtggatc agcgcttcct gttcggccag gcgctgtttc
     3301 agcaggaaga tgtcgtcaga agagatgtcg ttcataagcc cgtattttac cgggcttatt
     3361 ctgtgacaac caggataaag agatttacag catggtcagg gaggtcagca gccgcttagg
     3421 ctgtcgccag tcgatacctt ccagcagcat cgccagctgc gcctgcgtaa ggaacacttt
     3481 gccatcacgg gctgacggcc aggcgaagcg cccacgctcc agccgtttgg tcaggaggca
     3541 cagtccgtca ccggtggacc acagcagttt aacctgactg ccgctgcggc cccggaaaat
     3601 gaaaacatgg ccggacatgg gatcgtcttt cagcgccgtt tgtgctttcg cagccaggcc
     3661 gttgaagcca tttctcatat cggtgatacc ggcaaccagc caaattttgg tcccggaagg
     3721 taacgggatc atcgcttcag ttcctgtatc agtagagtca ggagcttttc gctgacattg
     3781 ccattgaagc ggagcgtccc gtgccggaac gttacctcac agctgatact gagggtttcc
     3841 gggtcctctg cgagcgattc tggctgttcg gcagcggcat cgagagtcac aggaagtagc
     3901 tgggggctct ctgaagaagg taatagcagc tttccctcgc gccattgttg tcgccatttg
     3961 aacaacagat tggcgttaat gccattttca agagcaagtt ttgagatgga tatcccgggt
     4021 tcacaggtgg cagcaacgag ctgctgttta aattcgggag gataattagg gcagcctttt
     4081 cgcctgccgg gagtcacatt tttctgcata tctgatactt tggttcccac tacttatttg
     4141 gtggacacca ctttgtctaa ttcgtcagat tctgaccaga cggttcaggc tgtacgctta
     4201 ccactatttt atccctgttg aatttggtta agccggtgca gacgcgccgg gaaacgcaca
     4261 cctgaaccgg cacaataaaa tacaatacaa ccccctgtta ctggagatac gaatggaatg
     4321 acgaggcagc ctgtcagtca cagggggctg ttggcataaa atcaacggaa aggccaccaa
     4381 caccggaatt aagctccaca gtccccgcac tggcaatcca ttcccgggaa aggcatcacc
     4441 gcaaactgct ccggaggcaa ccattacagc ctctgtaacg gattatcagc cctggcatct
     4501 tccgacggca cctcctgccg cccccacgcc ctgctgtata ccccggtacc gcagcgtggg
     4561 cagcactttt ccccctcata attttcgtca cacatcacac aaaaccactt tctcaccttc
     4621 accgtataat ccggaaccgt catgccctgc agatgcaaac gacgggcctc tttctctgcc
     4681 gggtcaagcc aggtacatcg cagcaggatt ttttcagcga cagtcatctg gtcatgttca
     4741 aaccacggtg gttgtccgtc attgccccgc tgaagtgtta acgggcatcc acaggacaca
     4801 cagcacaaaa cgtcctgtcg gttgtgcttc gcagcttctt tcgccgtaac aaaatggcct
     4861 ttgctgtcat tcgccatata acagttcaga attcgcatat ttcctcctga gttgtccggg
     4921 gaatactgaa tcctccggtc tggcagtgtg tgcagtactg ctccccataa taatcgtggt
     4981 gacattgtct gcagtgccag ctggctttac gcaccacggg taaggcatcc ggtacgaatt
     5041 gctgcagacg tttaatcaac tgtatttctc tgcgctccgg cctgacataa gggcactgtt
     5101 gaccgtgctc tgtcagcctg tcgtcagtgt gttcaaacca gggaagttca gtgtcgtatt
     5161 gcggatggta tctgagtgca ctgccacaca agtggcaggt gtagttagca taaggtgcgg
     5221 cctgtgcagt acgggcgccc gtcagacgtc cgttgccatc aagagcgata aaggattttg
     5281 cgtacatagt catatacctc agccgccaga cgacacgcag gcaacagccg tcccggtacg
     5341 ggcagcgtgg tcagggtgtg aatggcggtt cagttaaaat ctggactgga gtttgggtaa
     5401 agcaggctgg ttgctgttac agcactgtgg ttgtcacaca atggaacact gacgtaaaaa
     5461 acagtcagtg ggcgagtgga tacatcgttg ccgccagaca acaacccata cttttggttt
     5521 gccggatatt ggcgcgccag tcgtgctcaa ctccggctac agccaaaatt gcgctcaccg
     5581 gtgcagtgag ctttggtatg ctccttcgcc agatagtcag cacgttccag cacttgctga
     5641 aagccggtgt catcattacg cgccagccac accgccggcc aggccggaaa atgcaccagc
     5701 gtggcatggc acccgacatc cactcccagg gcactgcacc acgcctgttt aatcatcccg
     5761 gcgagagact ccggagcgcg gtaatcaccg gcacgacacc aggtatcccg gttgaccagc
     5821 agcatcaggt gatagtgttt tttacctttg cactccccaa actcccgggc ccagacgtaa
     5881 tgcagggttg tgggatgcac gcgtttacct tcccgatgct tacgtttctg gtaagcgtcg
     5941 attcgggctt tcagggcatt gatgaagcgg gatatcacag ctgcgtccgt tgctgccggc
     6001 acatccggga gacgcagatc aacccgaagt gccgtcaggc ggggatgaac atccagtgcg
     6061 tgccgcaccg tctcacgaat acgttgctgc cagaaggggt tgtatttgta ggtcatggtt
     6121 aaatctccgt atggttcata cggaatagcc acgtcgtaaa aaatacgcag agtccctgaa
     6181 gtgacctccg gcaggacact gtctcaggca ctctgtgata acccgaacgg tcgtctccgg
     6241 actgacggtt gaattttcag cgttgttttt taatgtaaaa aaccggttac gggtaaggct
     6301 gtgaggaggg agtgatggtg atacgcaaaa agaagtgcag ggactgtgga aacgcgatta
     6361 ctcataatac agtgtgttgc ccgtattgtg gtcctgctga ctactgtcag cgtcagtgtg
     6421 tgtatactgt gtagctggtg agataacggt tcatgtatcc cctgagctga caatgtaccg
     6481 tgtatattaa gcgtaaccga caggttctcc ttcataccat attaaatttt ccaggtattg
     6541 ccgctgcctt atttcgtccc atacaggaaa actcatctgg gtttctgaca aatacgcgaa
     6601 actcacagcc cctcaatatc aaccacacgt tgtaagccgg taatacctga aaatcagcct
     6661 attaccgccc acgcctccgg caccattccc ttttgatttc gaattctttg ttgccgttat
     6721 actgcttaat gttcccgatc ttccggtgca cagataacac cggagtcatc gtagcgcgga
     6781 ttcaagattc aggtgacata tttccagacg gaagccggca cgcgatcgta aagtttgttc
     6841 attgtcagtt ctgccagccg gtgatcgacc gcagagttaa aggccagttc cgcaggggta
     6901 agtttataac gtgtgtggga aatcactttt tccagcgtct cccgggatga acaacgacgg
     6961 aactgataca gccagtcttc tttggtttta acttccatta gcctctcatc actttatgct
     7021 gcaattaacg tgatactgcc agcataccgc acctgactgg cattctggta cccaccccaa
     7081 actcattact cccgcaccac cacttaaccg gtctctcaga ctgtcactca ccaggtcgga
     7141 tacgtttacc gctcagacgg atgcttaatg cactctgttc acgtgtgttg caggtacgtt
     7201 ttatatacgc aggttatacg ccagctatca gggatagccc tgtgagtaca acgtaatctg
     7261 ttgctactgc gttctcatac ccactgatca cactcacagt gtttatttca cagatatgaa
     7321 ctcatgtgtt tgatctctct tatatattta taataccaat cagacagtct tatcccatcg
     7381 gtgatacctg tctctgccca caaggcacga gctgccccac cgtaaccgcc ccgtaaataa
     7441 ctctgcaatt ataagaacga cggttcttcc cctcatcctc ccgaaaatca tcccttctct
     7501 gtcgtgcgta cgcgcgagaa acatcgtttc gcggtagcca ccgggtatcc cagcatcatg
     7561 ctccctgttt atctcccgac aggaatattc ccctgctcat tttttacgtt tctccccggt
     7621 tgttatgcca gtgaaggtgg tgcgtttttc atcaccaacg ccggttgaga ttaacaacat
     7681 ccggaggaac attttcatga ataccccagt ttcgctgatg gatgaccaga tggtcgacat
     7741 ggcgtttatc actcaactga ccggcttaac cgataagtgg ttttacaagc tcatcaagga
     7801 tggtgccttt ccggccccca tcaaactcgg ccgcagctcc cgatggctga aaagtgaagt
     7861 ggaagcctgg ttgcaggcgc gtattgcaca atcccgtccg taattcctga ccattacccg
     7921 ttcaaccgca gcgaatacac tatccggcct ccggaggcat tttgctgcgt gctaaaggag
     7981 agaatattat gcaaaaactt gaaaaccggt gtgatctgct gctcacccag catcagaaat
     8041 ggatggcaag tgtaacccgg tttattgtcg ctcacggaat gggctctccg catcttcatg
     8101 gttatcatcg cctgaccctg gcccactttt ttctgccgga aaaaggcacc atcatctcgg
     8161 tggcaccaca gggactctat caggtagtca acccggcaac gccaccgttc attcctgcca
     8221 ttcaggaagg gttaatgacc agcattcaga ctcatgaaat catgttactt acacatttta
     8281 acctgggtgg tgttttactc agtgaactac atcgtctggg agaaagccga ctggcaaaca
     8341 ggctgaacag tctgctccgg cgttttgacg atcgcgatct ctaccacacc ctgatatggc
     8401 tgtgctggta tgatctgatg tgcgcccact ccatgcagcc ctggactgaa gagttaaagc
     8461 ataaatcaca cgcagaactt gaaagctggg ctgtggcgcg caaacgggaa aagcgcgagc
     8521 ttgaactgat gattgacgag tacctgttat acgcctgttg attcataatc actcccgggc
     8581 ggtacgtagt cagtgatacc ggaatttccg gagagggtcg caggtatatt gtttctgtgc
     8641 aattcatggt tattcacgtt ggatgtagtg attgctgcat ccaatgctat cggaaagaga
     8701 ttaaccagca aatggttcct caaatcgtat tgatatttac ctggcgcatt gagcaccacc
     8761 atctgccccc gttcccacat caaacggctg gaccggaaaa cgatttgctt ttcatgctcc
     8821 gtggaaattc acgaaaaagt tattgggtca ttcatcgaaa aaaacatatt ctgctgactg
     8881 gagtcatcac ccagaaataa acgtcaactg aacaataacg attttttctt tgtgggattc
     8941 catacacttt tccatccccc tctgaaaatt agtcatcaac tcaccaacac atcttctgaa
     9001 atttgtatta aacttattgc ttaaatagat gatataatat ctcttccatt gcacggaatc
     9061 agcaaaaact gtcattctgg caatcttaat aagaacattt atcggttaat tttatcagta
     9121 aacaatggaa tatcatactg ataaaatgaa cattcagggt aagatgatga aattcacatg
     9181 aaaaatgctg aagattattt atttcatgca gataaacgat aaaaaacaag agagtatatt
     9241 tttcagtgta agagtcaact gttcatctcc actgacaatc acggtcagca tacaaacaaa
     9301 gtgagtcctc ctgcttagtc attgcgtatc agaaactaaa aaacaaaaga ttctgcaaac
     9361 aacgtgacat ttttatgaca tttcccgaat acatgtatat caataaacgg tagtcatcgt
     9421 tgaattaata agtcttacag agctatacgt agcttataaa accactcaac gaccatcatc
     9481 acctgactat ttagcgggag tatcgtctgt ataaatccat aaataataac acctgttatt
     9541 aaaataagaa tgctgtcagc cgtaaactat taacacaaca cctgtccact ctatctgttc
     9601 ttttaactca accggcgtcg aaaatatttc ctgattagtg catcatagtc ttcttttttc
     9661 tttctcaacc atcaaaccaa acacaacatt cacaaccaca caataaaaca aaaccaaaac
     9721 ataagattaa caatgccagt caacaggaaa acactgaata ttgaggtgcg ctgtaacctg
     9781 gacagcgcat acggcgcatg tgatcaacac agcccattct tttctgtcat cccccaaatg
     9841 cagaacaaac aatcataacc aaatgaatat aaaagaaaat atgatttttc accatcataa
     9901 aaacatcaat gtatctttat ataaaaacac tgataacatg acatagacat ttaaaaacat
     9961 aaacaaatca caacaaaaac atttgataat cacttagtga caaaataaaa ttgagacgat
    10021 tttgttataa gagaggcgac atggagtgag ataatgaata acgaacagat gcttatcaaa
    10081 tgcaaagatc cccgtaaatg tcggttttat gccgaatatc tgaatcacaa ataccccaat
    10141 gaattccctc agtttgcgcc tgacaaccca caaaactgta tcagaaatgt taattcgttt
    10201 ttaaaagaaa agtattataa agcttcaaac aattcttttg gtgtttttgg ttcgactgca
    10261 gaagaagctc tcagcaaaga aattaatgaa cttgaaatta ttgcagggca ggcaatactg
    10321 cctgaagaga gttttctctg gttgaaaact gacgaacttg cgactttctt tacctggact
    10381 actatttatc tgtcgaagga agaccataca aaattcccga aaataaacca taattcatca
    10441 ggaataaaaa acggaattga agtcaggaaa atacgaacag ttaccgatga aacgttaaat
    10501 acttacaaaa aaagtaacct tccggataat ccatcatccc ataacgaacg aataaaagta
    10561 atcatttcat attttgactc ctgccattct ttaattaata aaaatgcaaa aagcatatac
    10621 ctcgaggaac tcagacaggc gtggcttaaa aagaaaagcg cttgtcaaaa gtttaagtgg
    10681 ctccttcctg atgatgatga aatgtgtcag tggttctgga caagactgaa acaaaaacag
    10741 gatgaaaaac atccagtaca taacccagtg acccggtggt tcataccatc ttcaacgaaa
    10801 gaatattatc ttgcctctct tgtcgccttt gctttatgga aagaagcgcc tgataccatt
    10861 gaactgttca gaacacgaat aaatcatgcc tggcatcaga aaaaacaaaa ggacaaatat
    10921 cggagtgatg gtaaaaaggc cattaatata catataagaa gcaatgtgaa agaaatgctt
    10981 gatgagctca gaactgttta cggtctgaga acaggagagt tttttgaaat gctgattaca
    11041 gagaaatatc ttgaacacaa gcgaaggaag taattcatta cctgttacat tcta
//
LOCUS       DAJVUJ010000141        10903 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.141, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000141 DAJVUJ010000000
VERSION     DAJVUJ010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10903
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.141"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..185
                     /locus_tag="OQQ48_003291"
     CDS             <1..185
                     /locus_tag="OQQ48_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="interacts RssB preventing the degradation of the
                     stress sigma factor RpoS; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HCS1953949.1"
                     /translation="KLGIILQEKEYPITIYNIAPFNKNLWSLLKSSQECPPGESKITN
                     KCLHNSCIIKICSICY"
     gene            complement(253..1503)
                     /gene="icd"
                     /locus_tag="OQQ48_003292"
     CDS             complement(253..1503)
                     /gene="icd"
                     /locus_tag="OQQ48_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HCS1953950.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGEAIIENM"
     gene            1675..2328
                     /gene="rluE"
                     /locus_tag="OQQ48_003293"
     CDS             1675..2328
                     /gene="rluE"
                     /locus_tag="OQQ48_003293"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309634.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HCS1953951.1"
                     /translation="MRQFIISENTMQKTSFRNHLVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYTLDNLANGEWREATE"
     gene            2338..2799
                     /gene="nudJ"
                     /locus_tag="OQQ48_003294"
     CDS             2338..2799
                     /gene="nudJ"
                     /locus_tag="OQQ48_003294"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HCS1953952.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILKASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            2853..3959
                     /gene="mnmA"
                     /locus_tag="OQQ48_003295"
     CDS             2853..3959
                     /gene="mnmA"
                     /locus_tag="OQQ48_003295"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCS1953953.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCSVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            3995..4636
                     /gene="hflD"
                     /locus_tag="OQQ48_003296"
     CDS             3995..4636
                     /gene="hflD"
                     /locus_tag="OQQ48_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HCS1953954.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVPAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4640..6010
                     /gene="purB"
                     /locus_tag="OQQ48_003297"
     CDS             4640..6010
                     /gene="purB"
                     /locus_tag="OQQ48_003297"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCS1953955.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            6178..6849
                     /gene="phoP"
                     /locus_tag="OQQ48_003298"
     CDS             6178..6849
                     /gene="phoP"
                     /locus_tag="OQQ48_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HCS1953956.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            6849..8309
                     /gene="phoQ"
                     /locus_tag="OQQ48_003299"
     CDS             6849..8309
                     /gene="phoQ"
                     /locus_tag="OQQ48_003299"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HCS1953957.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            8385..9506
                     /gene="roxA"
                     /locus_tag="OQQ48_003300"
                     /gene_synonym="ycfD"
     CDS             8385..9506
                     /gene="roxA"
                     /locus_tag="OQQ48_003300"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HCS1953958.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(9555..10781)
                     /gene="pepT"
                     /locus_tag="OQQ48_003301"
     CDS             complement(9555..10781)
                     /gene="pepT"
                     /locus_tag="OQQ48_003301"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCS1953959.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLAGMKGTVERADMH
                     YIIRDFDRKQFEARKCKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
BASE COUNT         2697 a         2786 c         2726 g         2694 t
ORIGIN      
        1 ttaagcttgg tattatcctt caggaaaaag aatatcctat tactatttat aacattgcac
       61 cattcaacaa gaatttatgg agtttactca aaagcagtca agagtgtcct ccaggagaaa
      121 gcaaaataac aaataaatgt ttacataata gttgcattat aaaaatatgc tcgatatgct
      181 attaataata tccacataaa taaaacaacg ggcgtgttat acgcccgttt caatatttaa
      241 ctcatggaga gattacatgt tttcgatgat cgcttcacca aactctgaac atttcagcag
      301 cttagcgcct tccatcagac gttcaaagtc ataagttacg gttttcgcgt tgatagcacc
      361 ttccatacct ttaacaatta aatcagccgc ttcagtccac cccatatggc gcagcatcat
      421 ctcagcggag aggatgatag agcctggatt tactttgtcc tgaccggcgt atttcggcgc
      481 agtaccgtgg gtggcttcaa acagggcgca ttcgtcaccg atgtttgctc caggagcgat
      541 accgataccg ccaacctgcg ctgccagggc gtcagaaatg tagtcaccgt tcaggttcat
      601 acaggcgata acatcatatt cagccggacg cagcaggatc tgttgcagga atgcatcagc
      661 aatcacgtct ttaatgacga tctctttgcc ggtgttcggg tttttaactt tcagccacgg
      721 gccgccgtcg atcagttcac cgccaaactc ttcacgcgcc agctggtagc cccagtcttt
      781 aaacgcgcct tcggtgaact tcatgatgtt gcctttgtgc accagagtca cagagtcacg
      841 atcgttagca attgcgtatt cgatcgctgc acgaaccaga cgtttggtgc cttcttccga
      901 acacggctta ataccgatac cacaatgttc cgggaagcga attttcttca cccccatctc
      961 ttcacgcagg aatttaatca ctttctcggc gtcggcagag tctgctttcc attcgatacc
     1021 cgcataaatg tcttccgagt tttcacggaa gataaccata tcggtcagtt cagggtgttt
     1081 aaccgggctt ggagtgccct gatagtaacg taccggacgc aggcagatgt agagatccag
     1141 ttcctggcgc agggcaacgt tcagagagcg aataccgcca ccaacaggag tggtcagcgg
     1201 acctttaatg gcaacgcgat attcacgaat cagatcaagg gtttcagcag gcagccagac
     1261 atcctgacca taaacctgtg tggatttttc accggtgtaa atttccatcc aggagatttt
     1321 acgctcgcct ttataggctt tctcgactgc agcgtcgacc actttcagca tggctggggt
     1381 tacatctaca ccgattccat caccttcaat gtaagggata atcggatttt caggaacgtt
     1441 gagtttgccg ttttgcaggg tgatcttctt gccttgtgcc ggaacaacta ctttactttc
     1501 cattcacctc tccttcgagc gctactggtt tgctcgtctg ccaccacgtt gcatatgcgt
     1561 ttgcgtcctg cgatacggat gctttagagc aattttttgt taatgatttg taattggctt
     1621 gtcaatacta ccctattgtt tgccgtcatg aaagatgcgc gttattaggc tataatgcgg
     1681 caattcataa tctctgaaaa taccatgcaa aaaacttctt ttagaaatca cctggttaag
     1741 cgattcagct cgcaacgttc taccaggcgt aaacctgaaa accagcccac gcgtgtgatc
     1801 ctgttcaata aaccctacga tgttcttccg cagttcaccg atgaagccgg acgcaaaaca
     1861 ttaaaagaat tcatcccggt tcagggcgtt tatgcagcag gtcgtcttga ccgcgatagc
     1921 gaagggttac tggtgctgac caataacggc gcgttgcagg cgcgtttaac ccagccgggc
     1981 aaacgcaccg ggaaaatcta ttatgtgcag gtggaaggta ttcccacaca agacgcactt
     2041 gaagccttgc gcaatggcgt aaccttaaat gatggcccta ccctgcccgc cggcgcggag
     2101 ctggttgacg aaccagcgtg gttatggccg cggaatccac caattcgtga acgcaaaagt
     2161 attcccacca gctggctgaa gatcacctta tatgaaggac gtaatcgcca ggtgcgccgc
     2221 atgaccgccc atgttggttt ccccacgctg cgactgattc gttatgcgat gggtgattac
     2281 actttggata accttgccaa tggcgaatgg cgagaagcaa cagaataagg atgcacaatg
     2341 tttaaaccac acgttaccgt tgcttgcgta gtgcacgcag agggtaaatt tttagtcgtt
     2401 gaagagacga ttaatggtaa agcgttatgg aaccaacctg ccgggcatct ggaagccgat
     2461 gaaaccttag tggaagctgc cgcccgtgag ctatgggaag aaaccggcat cagcgcgcaa
     2521 ccgcaacact ttattcgtat gcatcagtgg attgcgccag ataaaacgcc gtttttgcgt
     2581 ttcctctttg ccattgagct tgagcaaata tgcccgaccc agcctcatga cagcgatatc
     2641 gactgctgtc gttgggtcag cgccgaagaa attttaaagg cgtcaaatct tcgttcgccg
     2701 ctggtggcgg aaagtatccg ttgttatcaa agcgggcaac gctatccgct ggagatgatt
     2761 ggcgatttta actggccttt tacaaagggt gtcatctaaa aggctgcgtg atagaatacg
     2821 ccgccttgaa gttcaatgtc gtgagtgatc caatgtctga aaccgcaaaa aaagtaatcg
     2881 tcggcatgtc cggcggtgtc gattcctccg tttctgcctg gctgttgcaa caacagggat
     2941 atcaggttga aggcctgttt atgaagaact gggaagaaga cgacggtgag gaatattgca
     3001 cagcggcagc ggatctggct gatgcccaag ctgtctgcga caagctcggc attgaactgc
     3061 acaccgttaa ctttgctgcc gagtactggg acaacgtctt cgaactgttc cttgccgaat
     3121 ataaagccgg tcgcacgccg aatccggata ttctgtgcaa caaagagatc aaatttaaag
     3181 ctttcctcga atttgccgcc gaagatttag gtgccgatta tatcgctacc ggtcattacg
     3241 tacgtcgggc cgatgtcgat ggcaagagcc gcctgctgcg tggtctggac agcaataaag
     3301 accagagcta cttcctttat acgctcagcc atgagcagat tgcgcaaagc ctgttcccgg
     3361 tcggcgaact ggaaaaaccg caggtgcgta agattgctga agatcttggt ctggtcaccg
     3421 cgaagaaaaa agactctacc ggcatttgct tcattggcga acgtaaattc cgcgagttcc
     3481 tgggccgtta tctcccggcg caaccgggca aaatcattac cgtcgatggc gatgaaattg
     3541 gtgagcacca ggggctgatg tatcacactc tcggtcagcg taaaggtctg ggtatcggtg
     3601 gcaccaaaga aggcaccgaa gaaccgtggt atgtggtgga caaagacgtc gaaaacaaca
     3661 ttctggttgt tgcgcagggc catgaacacc cgcggctgat gtctgtcggg ttgattgccc
     3721 agcagttgca ctgggtcgat cgcgaaccat tcaccggtac tatgcgttgc tcggtaaaaa
     3781 cccgctatcg ccagaccgat atcccttgca ccgtcaaggc gctggacgat gatcgcattg
     3841 aagtgatttt cgatgaaccg gttgccgccg tgacgccggg ccagtctgcc gtcttctata
     3901 acggtgaagt gtgcctcggt ggcggtatta ttgagcagcg tctgccgctg ccggtctgat
     3961 tattatcttt acttaaaaca aggaagcagt gaacgtggca aagaattact atgacatcac
     4021 cctcgccctg gccggtattt gtcagtcggc acgcctggtg caacaactcg ctcaccaggg
     4081 gcattgtgat gccgatgcgc tacacgtctc actcaacagt attattgata tgaaccccag
     4141 ctcgacgctg gcggtttttg gcggtagcga agccaacctg cgcgtcgggc tggaaaccct
     4201 gctcggcgtg ctcaatgcca gcagtcgcca gggcttaaac gccgaattaa cccgctacac
     4261 actcagcttg atggtgcttg agcgcaaact ctcctcagcg aaaggcgcgc tcgacactct
     4321 gggcaaccgg atcaacggcc tgcaacgcca gctcgaacac ttcgatttac agtccgaaac
     4381 gctgatgagc gcgatggctg ctatctatgt tgatgtgatc agcccgcttg gcccgcgcat
     4441 tcaggtcacc ggttcccctg ctgtactgca aagcccacaa gtgccggcga aagttcgcgc
     4501 aaccctgctg gcaggcattc gcgccgccgt gctctggcac caggtcggcg gcggacgtct
     4561 gcaactgatg ttttctcgta atcgcctgac cactcaggca aaacaaattc ttgctcattt
     4621 aaccccggag ttgtgatcta tggaattatc ctcactgacc gccgtttccc ctgtcgatgg
     4681 acgctacggc gataaagtca gcgcgctgcg cgggattttc agcgaatatg gtttgctgaa
     4741 attccgtgta caagttgaag tacgttggct gcaaaaactg gccgcgcacg cagcgatcaa
     4801 ggaagttcct gcttttgctg ccgacgcaat cggttacctt gatgcaattg tcgccaattt
     4861 cagcgaagaa gatgccgcac gcatcaaaac catcgagcgt accactaacc acgacgttaa
     4921 agcggttgag tatttcctga aagaaaaagt ggcggagatc ccggaactgc acgcggtttc
     4981 tgaattcatc cactttgcct gtacttcgga agatatcaat aacctctccc acgcattaat
     5041 gctgaaaacc gcgcgtgatg aagtgatcct gccgtactgg cgtcaactga ttgatggaat
     5101 taaagatctc gccgttcagt accgcgatat cccgctgctg tctcgtaccc acggtcagcc
     5161 agccacgccg tcaaccatcg gtaaagagat ggcaaacgtc gcctaccgta tggagcgcca
     5221 gtaccgccag cttaaccagg tggagatcct cggcaaaatc aacggcgcgg tcggtaacta
     5281 taacgcccac atcgccgctt acccggaagt tgactggcat cagttcagcg aagagttcgt
     5341 cacctcgctg ggtattcagt ggaacccgta cactacccag attgaaccgc acgactacat
     5401 tgccgaactg tttgattgcg ttgcgcgctt caacaccatt ctgatcgact ttgaccgtga
     5461 cgtctggggt tatatcgccc ttaaccactt caaacagaaa accattgctg gtgagattgg
     5521 ttcttccacc atgccgcata aagttaaccc gatcgacttc gagaactccg aagggaatct
     5581 gggcctttcc aacgcggtat tacagcatct ggcaagcaaa ctgccggttt cccgctggca
     5641 gcgtgacctg accgactcca ccgtgctgcg taacctcggc gtgggtatcg gttatgcgct
     5701 gattgcgtat caatccaccc tgaaaggcgt gagcaaactg gaagtgaacc gtgaccatct
     5761 gctggatgag ctggatcaca actgggaagt gctggctgag ccaatccaga cagttatgcg
     5821 tcgctatggc atcgaaaaac cgtacgagaa gctgaaagag ctgactcgtg gtaagcgcgt
     5881 tgacgccgaa ggcatgaagc agtttatcga cggtctggcg ttgccagaag aagagaaagc
     5941 ccgcctgaaa gcgatgacgc cggcaaacta cattggtcgc gccatcacca tggttgatga
     6001 gctgaaataa actcgtatca gtgccggatg gcgatgctgt ccggcctgct tattaagatt
     6061 atccgctttt tattttttca ctttacctcc cctccccgct ggtttattta atgtttaccc
     6121 ccataaccac ataatcgcgt tacactattt taataattaa gacagggaga aataaaaatg
     6181 cgcgtactgg ttgttgaaga caatgcgttg ttacgtcacc accttaaagt tcagattcag
     6241 gatgctggtc atcaggtcga tgatgcagaa gatgccaaag aagccgatta ttatctcaat
     6301 gaacatttac cggatattgc gattgtcgat ctcggattgc cagacgagga cggtctgtca
     6361 ctgattcgcc gctggcgtag caatgatgtt tcactgccga ttctggtatt aaccgcccgt
     6421 gaaagctggc aggacaaagt cgaagtatta agtgctggtg ctgatgatta cgtgactaaa
     6481 ccatttcata ttgaagaggt gatggcgcga atgcaggcat taatgcggcg taatagcggt
     6541 ctggcttcac aggtcatttc gctaccaccg tttcaggttg atctctctcg ccgtgaatta
     6601 tctattaatg acgaagtgat caaactgacc gcgttcgaat acaccattat ggaaacgttg
     6661 atacgcaata atggcaaagt ggtgagcaaa gattcgttaa tgctccaact ctatccggat
     6721 gcggagctgc gggaaagcca taccattgat gtactgatgg gacgtctgcg caaaaaaatt
     6781 caggcacaat atccgcaaga agtgattact accgttcgcg gccagggcta tttgttcgaa
     6841 ttgcgctgat gaaaaaatta ctgcgtcttt ttttcccgct ctcgctgcgg gtacgttttc
     6901 tgttggcaac ggctgcggta gtactggtgc tttcgcttgc ctacggaatg gtcgcgctga
     6961 tcggttatag cgtcagtttc gataaaacta cgtttcggct gttacgtggc gagagcaatc
     7021 tgttctatac ccttgcgaag tgggaaaaca ataagttgca tgtcgagtta cccgaaaata
     7081 tcgacaagca aagccccacc atgacgctaa tttatgatga gaacgggcag cttttatggg
     7141 cgcaacgtga cgtgccctgg ctgatgaaga tgatccagcc tgactggctg aaatcgaatg
     7201 gttttcatga aattgaagcg gatgttaacg ataccagcct cttgctgagt ggagatcatt
     7261 cgatacagca acagttgcag gaagtgcgag aagatgatga cgacgcggag atgacccact
     7321 cggtggcagt aaacgtctac ccggcaacat cgcggatgcc aaaattaacc attgtggtgg
     7381 tggataccat tccggtggag ctaaaaagtt cctatatggt ctggagctgg tttatctatg
     7441 tgctctcagc caatctgctg ttagtgatcc cgctgctgtg ggtcgccgcc tggtggagtt
     7501 tacgccccat cgaagccctg gcaaaagaag tccgcgaact ggaagaacat aaccgcgaat
     7561 tgctcaatcc agccacaacg cgagaactga ccagtctggt acgaaacctg aaccgattgt
     7621 taaaaagtga acgcgaacgt tacgacaaat accgtacgac gctcaccgac ctgacccata
     7681 gtctgaaaac gccactggcg gtgctgcaaa gtacgctgcg ttctctgcgt agtgaaaaga
     7741 tgagcgtcag tgatgctgag ccggtaatgc tggagcaaat cagccgcatt tcacagcaaa
     7801 ttggctacta cctgcatcgt gccagtatgc gcggcgggac attgctcagc cgcgagctgc
     7861 atccggtcgc cccactgctg gacaatctca cctcagcgct gaacaaagtg tatcaacgca
     7921 aaggggtcaa tatctctctc gatatttcgc cagagatcag ctttgtcggt gagcagaacg
     7981 attttgtcga ggtgatgggc aacgtgctgg ataatgcctg taaatattgc ctcgagtttg
     8041 tcgaaatttc tgcaaggcaa accgacgagc atctctatat tgtggtcgag gatgatggcc
     8101 ccggtattcc attaagcaag cgagaggtca ttttcgaccg tggtcaacgg gttgatactt
     8161 tacgccctgg gcaaggtgta gggctggcgg tagcccgcga aatcaccgag caatatgagg
     8221 gtaaaatcgt cgccggagag agcatgctgg gcggtgcgcg gatggaggtg atttttggtc
     8281 gccagcattc tgcgccgaaa gatgaataaa tatgtccata cttcacgcat tacgttaagc
     8341 atccgttata atcggttgca gataccagcc tgtggatgct taacatggaa taccaactca
     8401 ctcttaactg gcccgatttt cttgaacgtc actggcagaa acgcccggtg gtgttaaaac
     8461 gcggctttaa taattttatt gacccgatct ctccagacga gttggcgggt ctggcgatgg
     8521 aaagcgaagt tgacagtcga ctggtcagtc accaggatgg caaatggcag gtcagccacg
     8581 gcccgttcga aagctacgat catctcggtg aaaccaactg gtcattactg gtacaggcag
     8641 tgaaccactg gcatgagccg accgccgcgc tgatgcgacc gttccgtgaa ctaccggact
     8701 ggcgtattga tgatctgatg atttcttttt ctgtacctgg cggcggcgtc ggcccgcatc
     8761 tcgatcagta cgacgtgttt atcattcagg gtaccggacg tcgtcgctgg cgagtgggcg
     8821 aaaagctgca aatgaaacag cactgcccac atccggatct gttacaggtc gatccgttcg
     8881 aagccatcat cgatgaagag ctggagcctg gtgatattct ttatattccg ccaggattcc
     8941 cgcatgaagg ctacgcgctg gaaaatgcga tgaactattc cgtgggcttt cgcgcgccaa
     9001 atacgcggga actgattagt ggatttgccg attatgtgct gcaacgtgaa ctgggcggca
     9061 actactacag cgatcctgat gttccacctc gcgctcatcc tgcggatgtt ctgcctcaag
     9121 agatggataa actgcgtgag atgatgctcg aattgatcaa ccagccggaa cactttaagc
     9181 aatggtttgg cgagtttata tcccagtcac gtcatgaact ggatatcgcg ccgccggaac
     9241 cgccttatca gccagatgaa atctacgatg cgctgaaaca aggtgatgtg ctggtgcgcc
     9301 tgggtggtct gcgcgtattg cgcattggcg acgacgtgta tgccaatggt gagaagatcg
     9361 attccccgca ccgtccggca ctggatgcac tcgccagcaa cattgcgctg actgcggaga
     9421 attttggcga tgcgctggaa gatccgtcat tcctcgcgat gctcgcggcg ctggtcaata
     9481 gcgggtattg gttcttcgaa gggtaagttt ggatttaagc cggatgcggc atcacatgcc
     9541 gcatcccttt tcgcttactt ccgttgcgcc gttaactcgg caatacggac gatcacctgc
     9601 accgcttttt ccataccttc cagagtcaca aactcatgct taccatgata gttgtaaccg
     9661 ccagtgaaca agttcgggca cggtaatccc ataaacgaca actgcgcgcc gtcggtaccg
     9721 ccgcggatcg gtttcagttc cggttcaata tcgcagtcac gcattgcctg ctgggcgata
     9781 tcgagaatat gcggatgctc aaccactttc tcgcgcatat tgtagtaact gtcttcaatc
     9841 accagttcaa tgtagcaatc aggatgtaac cctttgccca cttttttggc gatctccatc
     9901 attttacatt tacgcgcttc aaactgttta cggtcgaaat cacggatgat gtagtgcata
     9961 tcggcccgtt caacggtgcc tttcatgccc gccaggtgat agaaaccttc atagccttct
    10021 gtcatttccg ggctttcatc cgccggaact tccgcatgaa tacgtgccgc cagcgacagc
    10081 gcatttacca tcactccttt cgccgtgccc ggatgaacat tgttaccgac aattttgata
    10141 ttgaccgatg cggcgttgaa gttttcaaac tccagttcgc ctacgccacc accgtcaaca
    10201 gtgtaagccc agcgggcatc gaaggcatca acatcaaaat gtttcgcccc tttgcccact
    10261 tcttcatccg gggtaaaggc gacgcgaata tcaccatgcg gaatgttttt ctgttgcaat
    10321 accgccagcg cggtcatgat ttctgcaata cctgctttgt catcggcacc taacaaggtt
    10381 ttaccatcgg tggtaatcaa tgtctgaccc agtagctgat gcagcaccgg gaacataacc
    10441 ggtgataaaa cttcatcgcc gatacccagc gcaatatcgc caccgcgata gttttcaaca
    10501 atttgcggat taacattttt gccgctgcaa tccggtgagg tatccacatg agaaataaag
    10561 ccaatcgccg ggatatcgcc agggacgtta gccggtaacg tcgccatcaa agtgcccttc
    10621 tcacttaagg tcacattgat aagccccatt tcttcgagct gctctttcag cagatgcaat
    10681 aacttccatt ggccttccgt gctgggaacc tgtctcaccc ctgcttttga ttgggtatcc
    10741 agagacacgt agttcaaaaa tcgctcaagt agtttatcca tgtagtcacc ctcacttttt
    10801 gtgacaacat tattaagaag caagaaaaga caaatattgc gtcaggtcac ttttacccct
    10861 gcaagcggga atatttatca gcattacctt aatgaatata aag
//
LOCUS       DAJVUJ010000142        10822 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.142, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000142 DAJVUJ010000000
VERSION     DAJVUJ010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10822
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.142"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..145
                     /locus_tag="OQQ48_003302"
     CDS             <1..145
                     /locus_tag="OQQ48_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HCS1953960.1"
                     /translation="KAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQL
                     IAN"
     gene            211..1008
                     /locus_tag="OQQ48_003303"
     CDS             211..1008
                     /locus_tag="OQQ48_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HCS1953961.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSAFGVQALRVAIPALIVSL
                     FVSADMVSNMLSAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            1019..1861
                     /locus_tag="OQQ48_003304"
     CDS             1019..1861
                     /locus_tag="OQQ48_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000437910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCS1953962.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITTALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGST
                     VTMTVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLSRF
                     FAPA"
     gene            1895..3124
                     /gene="sorE"
                     /locus_tag="OQQ48_003305"
     CDS             1895..3124
                     /gene="sorE"
                     /locus_tag="OQQ48_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HCS1953963.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRMLILGGTGPMGLLAIDYALHGPVNPSQLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNAADAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLTHAEAISHD"
     gene            3507..4196
                     /gene="pepE"
                     /locus_tag="OQQ48_003306"
     CDS             3507..4196
                     /gene="pepE"
                     /locus_tag="OQQ48_003306"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HCS1953964.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(4287..5918)
                     /locus_tag="OQQ48_003307"
     CDS             complement(4287..5918)
                     /locus_tag="OQQ48_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HCS1953965.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTAALTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQSDEDEGRDEY"
     gene            complement(6138..9821)
                     /gene="metH"
                     /locus_tag="OQQ48_003308"
     CDS             complement(6138..9821)
                     /gene="metH"
                     /locus_tag="OQQ48_003308"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HCS1953966.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLV
                     AAYCESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEAQDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTDNAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNCIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            10021..>10822
                     /gene="iclR"
                     /locus_tag="OQQ48_003309"
     CDS             10021..>10822
                     /gene="iclR"
                     /locus_tag="OQQ48_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HCS1953967.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMEVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVT"
BASE COUNT         2456 a         2966 c         2855 g         2545 t
ORIGIN      
        1 caaagcggtt tcattagatg atgatgatat taacgcattt catgagttaa ataatcttgg
       61 cgtcatactg gatttacgcg tcgtagcgtc agatccctca atcaatatta tcgacaaaat
      121 aaacgaacag ttaatcgcaa attaaataat agcgcttgtg tacatatgct ttaacaggca
      181 gggatgacga cacttaaaag gtgaaaaatc atggaaataa gtaccctaca aatcattgct
      241 atatttcttt tttcctgtat tgccggaatg ggcagcgtgc tggatgaatt tcagactcat
      301 cgcccgttaa ttgcctgtac ggtgattggt ttaattctcg gtgatttaaa aaccggaata
      361 atactcggtg gtacgctgga attgatagct ctcggctgga tgaacgtcgg cgcggcgcaa
      421 tctccggatt ctgcactcgc cagcataatc tccgccattc tggttatcgt tggtcagcaa
      481 agcatcgcca ccggaatcgc catcgcgttg cctgtggctg cggcaggcca ggtgctgact
      541 gtgtttgccc gtaccatcac cgtagtgttc cagcacgcgg cggataaagc agcggaagaa
      601 gcacggtttc gcactctcga tattctgcat gtctccgcgt ttggcgtgca ggcgctgcgc
      661 gttgctattc cggcactgat tgtctcactg ttcgtcagcg ccgatatggt gagcaatatg
      721 ctgagcgcga taccggaatt cgtgacgcgc ggtctgcaaa ttgctggcgg ttttatcgtg
      781 gtggtcggtt acgccatggt gctacgtatg atgggcgtga aatatttgat gcctttcttt
      841 ttcctcggtt tcctcgcagg tggctacctc gatctcagtc tgctggcgtt cggtggcgtc
      901 ggcgtgatca tggccctggt ctacatccag ctaaatccac agtggcgtaa agctgaacct
      961 caactccagg ccaccgcctc caccgccctt gaccaacttg acgattaacg gagccatcat
     1021 ggaacagaga aaaattacac gcagcgatct ggtgagcatg tttctgcgct ccaacctgca
     1081 acaggcgtcc tttaactttg aacgtattca cgggctgggt ttttgctacg acatgatccc
     1141 cgccatcaaa cgactttacc cattaaaaga ggatcaggtt gcggcgctca ggcgacacct
     1201 ggtgttcttc aataccacgc cagccgtatg tggcccggtc atcggcgtca ctgccgccat
     1261 ggaagaggcg cgggccaacg gcgcggaaat tgatgacggt accattaacg gcatcaaagt
     1321 cggtctgatg ggaccattgg caggagttgg cgatccactg gtctggggaa cgctgcgccc
     1381 gattaccacc gcgctcggcg catctctggc actttcgggc aacattctcg gcccgctgct
     1441 gttcttcttt attttcaatg cggtgcgtct ggcgatgaag tggtatggcc tacagctcgg
     1501 ctttcgcaaa ggggtgaata tcgtcagcga tatgggcggg aatgtgctgc aaaaactcac
     1561 cgaaggcgcg tcgattctcg gactgtttgt gatgggcgtg ctggtcacca aatggacgtc
     1621 aatcaacgta ccgttggtgg tttcacaaac gcatgccgcc gatggctcca ccgtcaccat
     1681 gaccgtgcag aacattctcg accaactttg ccctggtttg ctggcgctcg gtctgacgct
     1741 gcttatggtt cgtctgctca acaaaaaaat taacccggta tggctgattt tcgccctgtt
     1801 tggcttaggg attatcggta atgcgctggg cttcctgtcc agattcttcg ccccggcatg
     1861 actgccgggg ccatcgctca acatgaggtg gtttatgaaa acaacagctc tgcgtcttta
     1921 tggtaaacgt gatttacgcc tggaaacctt tgaccttcct gaaatgcagg aggatgaaat
     1981 cctcgcgacg gtggtcactg acagcctgtg cctctcttcc tggaaagagg ccaatctggg
     2041 tgaaaaccat aaaaaagtac ccgacgatgt ggcgaccaac cccatcatca tcggccacga
     2101 gttttgcggc gatattctgg ccgtgggtaa aaagtggcag cacaaattcc agccgggtca
     2161 gcgttatgtg attcaggcca acctgcaact ccccgaccgc ccggactgcc ccggctactc
     2221 cttcccgtgg gtgggcggcg aagccacgca tgtagttatt cccaacgagg tcatggaaca
     2281 agattgcctg ctggcatacg acggcgaaac ctattttgaa ggctcactgg ttgaaccgct
     2341 ttcctgcgtg attggcgcgt tcaacgccaa ctatcatctt caggaaggta gttataacca
     2401 cacgatgggg attcgcccgc aagggcgcat gctgatcctc ggcggcaccg gaccaatggg
     2461 actgttggcg attgattatg cgctacatgg acccgttaac ccgtcgcagc tcgtcattac
     2521 cgataccgac aacgataaat tgagttatgc gcgcaagcac tatccatcag aaccgcaaac
     2581 actgattcat tatctcaatg ccgccgatgc agcatttgat acgctaatgg cgctgagtgg
     2641 cggtcacggc ttcgatgata ttttcgtctt tgtgcctaat gaaggactgg tgactctcgc
     2701 ctcttccttg ctggcgacag atggttgcct gaatttcttc gccggaccgc aggataaaca
     2761 tttcagcgcg ccaattaatt tctacgatgt gcattatgca tttacccact acgtgggcac
     2821 gtcaggcggc aataccgacg acatgcgcgc ggcggtcaaa ctgattgaag agaaaaaagt
     2881 gcaggccgca aaagtggtaa cacatattct tgggctgaat gccgcgggcg aaaccacgct
     2941 tgaattgcct gccgtcggcg gcggcaaaaa gctggtgtat accgggaaat acctgccgct
     3001 gacgtcactc acgcagattc aggatcaagc actggcggcg attctggcgc gtcatcaggg
     3061 gatctggtcc ggtgaggcgg agcaatatct gctcactcat gcagaggcaa tttcccatga
     3121 ttaatcgcgc tacttaactc tgcatgtcgc tggctggtcg ccgcggcaac ttcgcgacct
     3181 gatgtgcggc tgattgctga agcggcccaa tgtgcacgag aaggataaaa aaacatcagc
     3241 gccttaaggg gcgctcaaat tggtggcaaa gtacacattg tttatgccgg atgcggaata
     3301 aagccttatt cggcctacat aatctcgaaa attcaatata ttgcagttat ctggtaggcc
     3361 tggtaagcgt agcgcatcag gcaatgctgt gtttgtcatc agtctcagct cccggtgcaa
     3421 tacgtgacat cgtgagccat gtcagtaagt ggttaaattc gcagtggtag catgccttcc
     3481 gtgatttctt actaaccgga gaagtaatgg aactgctttt attgagtaac tcgacgctgc
     3541 cgggtaaagc ctggctggaa catgcactgc cgctaattgc tgaacagttg cagggtcgcc
     3601 gctcagcggt gtttatccct ttcgctggcg taacgcagac ctgggatgat tacacagcga
     3661 aaacggctgc ggttctcgct ccgctgggtg tttctgtcac cggtattcat agcgttgtcg
     3721 atcccgttgc cgcgattgaa aatgctgaga tcgtgattgt cggcggcggg aatactttcc
     3781 agttgctgaa acagtgccgc gagcgcgggc tgctggcacc aattactgac gtggttaaac
     3841 gtggtgcgct gtatattggc tggagcgcag gcgctaacct tgcttgccca accattcgta
     3901 ccaccaacga tatgccgatt gtcgatccgc aaggtttcga tgcgctaaat ctgttcccgt
     3961 tgcaaatcaa cccgcacttc accaacgcgc tgccggaagg ccataaaggt gaaacccgtg
     4021 agcagcgtat tcgcgaactg ctggtcgtcg cgccagaact gacgattatt ggtctaccgg
     4081 aaggtaactg gatcacagtg agtaaaggtc acgctacgct gggtggcccg aacaccacct
     4141 atgtgtttaa ggccggtgaa gaagcggttc cgctggaagc tggtcaccgt ttttaagtga
     4201 attgccggat gcatgatccg gcatcttgtt catagtgcct gatgcgacgc ttgacgcgtc
     4261 ttatcaggcc tacaggattc acaatattaa tactcgtccc gtccttcatc ttcatccgac
     4321 tgttccagca cactgtaagc caccgaacaa aacagcgagt tcaggcgctg catatctccc
     4381 agtaagccta aatgtagcga actggtttca atgctctgca cgttttgctg atgcaggcga
     4441 tcgacgtggg cgtgggaata gcggcgatta agaatgcgaa agcgatgctt actacggcgc
     4501 aaacgacgag cgctggtgac atcgccagag aagaacaccg acattgccag ctttaaatta
     4561 ctgagcaatt gctcatagag cgcatccagt tccttcaacc catcaagcga aaatgcccgc
     4621 cgtgctgcca gtgatttgtc agcaatttcg ctgcccatgc gctcgacgat atcggaggcc
     4681 tgttcaaggt tgagcgacat ttcaataatc tccgcccagc ggcgcgactc ttcctccgcc
     4741 agctcttctt ttggcatccg cgccagatac agcttaatgg cggtgtagag aacgttgata
     4801 tcatctgcca gcttacgcag ctctttctcc tgccgtggct caccgtgcat cactttattc
     4861 aacccttcca tcatctgttc catggcgtcg ccaatgcgca gggtttcgcg cgcggcgttc
     4921 gccagagcaa gcgtgggcgt atccagcgcg ctgacatcca gatgtttagg ccgtagctgg
     4981 gtatccagtt ccggttcatc gcgaataatc gttttgcaaa accgtgccat cgggtcaaca
     5041 aatggcagca tgaccaggca acgtacaagg ttgtagaaga cgtggaaata gatcaccagt
     5101 tccgcttttg gcaatgacag cttccccatt gtctctgcca gcaaatggac aaacggcagg
     5161 ataatcaggc tacccaccag cttaaacagc agactaccca gcgcgacacg gcgggctgcg
     5221 gcattggcgg cactgttgtt gagcatcgcc agcaggccgg aaccgaggtt agcaccaatc
     5281 accagacaga gcgccacggg gaaggagata atgcctgcgg cggtcagagc cgcggtcagc
     5341 agtacagcag caaggctgga gtagctgata atggcgaaca tcgcgccaat cagcgcatcc
     5401 agcagaatat cgccggtcag cgaggcaaag atcacctgaa cgccgtttgc ctgggtgatc
     5461 ggcgttacgg cctgcacaat caactccagc gccagcaaaa tcagcccaag accaataccg
     5521 acgcggccca gttgcccggc gcgtgactgt ttgcgtccga ggaaaaaaat cacgccgata
     5581 aaaataagta acggtgacag ccaggataag tcgaaggtga gaatacgcgc cattagcgcc
     5641 gtcccgacat ctgcccccag cacaatgacc agagccggtg cgagggctac cagatcctga
     5701 gcgacgaacg aggtcaccag catggtggtg gcattactgc tctgtaccag tgcggtaacg
     5761 ccgatccccg cgcaaaaggc gagcggcttt ttttcgacgc tccggctaag gacagtacgc
     5821 aaacgcgcgc cgaagacgcg cattacgccg gttcgaacaa tatgagtccc ccagaccagc
     5881 agggcgacgg cagaaagcag gtgaagcagc gttaacacga gttcaggttc tccttatcgt
     5941 tatatgttcc tgaagcaatt gaatgaccaa aggaccggaa acacgctgac tggcatcttt
     6001 actgtgcctt ttgacattcg tttttccggc taagattatt ccagcctgat tccagtataa
     6061 gtgtttcata ttaataaaga gacagggcac tcattgagcg ccctgtttgt tgtaaggaaa
     6121 agtgacagat ttgtgaatca gtccgcgtca taccccagat tcggtgccag ccagcgctca
     6181 acatctgaga cgctcatacc tttacggcgg gcatagtctt caacctgatc gcgctgaatt
     6241 tgcgccacgg cgtagtactt gctgtccggg tggctgaaat accaacccga aaccgatgca
     6301 ccgggccaca tggcgaaaga ttctgtgagt ttcatgccgg tatgtttttc cacttccagc
     6361 agctcccaga tggtggcttt ttccgtatgt tccgggcagg ccgggtagcc cggtgccgga
     6421 cggatgccct ggtagttttc gcggatcagt tcttcgttgc tgaggttctc gttcggcgca
     6481 tagccccagt agactttacg cacacgctca tggagatact ccgcaaaggc ttcggctaaa
     6541 cggtcggcaa gcgctttcac catgattttg ttgtaatcat cgtgctgcgc ttcaaaggca
     6601 tcagccagtg cgtcctcttc cagcccgcca gtcacggcaa atgcgccgat gtaatctgct
     6661 ttaccagaaa gcttcggcgc aacaaagtca gcgagacagt agttagcgaa tcccgttttt
     6721 tcggtctgtt gacgcagatg gtggctgaca ttgatcacat gggtgcgcgt ttcgtcacgg
     6781 tagatttcaa tgtcatcgcc cacacggttt gccgggaaca ggcccaccac gccacgcgga
     6841 ttcagcgttt tctcggcgct taatttatcc agcatgtcgt tggcgtcttt aaacagccgc
     6901 tgcgcctcaa cgcccaccac ttcatcttcc agaatgcgcg gatacttccc ggccagcgac
     6961 caggtcataa agaacggtgt ccagtcgatg caattacgca gagtttcgat gctggcttca
     7021 acttcctgca cgccgagacg gtgcgccacc ggcggcgtgt aagcctgcca gtcaaaagcg
     7081 aagtcgttat cgcgcgccgc ttccagcgtg accggtggtg tgcgcggttt cttgcgcccg
     7141 tgctgaatac gtacggtttc gtactccttg cgggtacgag cgacaaaatc atcgcgctga
     7201 gtatcggaaa gcagcgccgc caccacacca acggtgcgcg aggcattctg cacatacacc
     7261 gtcgggccgc tgtagttctg ctcaattttc accgccgtgt gcgcttttga ggtggtcgcg
     7321 ccaccgatca gtaacggaat ggtgaagccc tgacgctcca tctctttcgc cacgttaacc
     7381 atctcgtcca gcgacggcgt gataagcccc gaaaggccaa tcaaatcagc attcacttct
     7441 ttagcagtac ggagaatttt ttccgcaggc accatcacgc cgagatcgac aatttcgtag
     7501 ttgttacatt gcagcaccac gccaacgata tttttaccga tatcgtggac gtcgcccttc
     7561 acggtggcga tcaccatctt gccgttggtt ttgccctgct ctttgctggc ttcaataaac
     7621 ggttcgagat acgccaccgc ctgtttcatg acgcgcgccg atttgaccac ctgtggcagg
     7681 aacattttgc cttcgccaaa cagatcgccg accacgttca tgccgtccat caacgggcct
     7741 tcaatcactt caatcgggcg cgtagcctgc tggcgggctt cttcggtatc ctgctcgata
     7801 aactcggtaa tgcctttgac cagcgagtat tccagacgtt tattcacttc ccacgagcgc
     7861 cactccgcct gctgggcgtt atcggtgtcg tcggttttgc tgccgcgata tttctcggca
     7921 agctccagta aacgctcagt gccatcgtca cgacgattaa gaatcacatc ttccaccgcg
     7981 tcgcgcagtt cagcgggtag gtcgtcgtaa atcgccagtt gccccgcgtt gacgatcccc
     8041 atatccatgc cattgcgaat agcgtagtag aggaacactg cgtgaatggc ttcgcgcacc
     8101 ggatcgttgc cacggaatga gaaagaaacg ttagatacgc cgccggaaat cagcgcgtgc
     8161 ggcagttcgc gtttgatgtc ttcacacgcg ccgataaagt cctgcgcgta gttgttgtgc
     8221 tcttcaatac cggtggcgac cgcgaagatg ttcgggtcga agatgatatc ttctggcggg
     8281 aagccaacct cttcggtgag gattttgtac gcccgacggc aaatctcgat tttccgtgcg
     8341 cgagtatcgg cctgtccctg ttcatcaaag gccatcacca ccactgccgc accgtagcga
     8401 cgcaacaatt tcgcgtgatg gataaaggcg tcgacgccct ctttcatcga gattgagtta
     8461 acaatgcctt tgccctgaat acacttcagc cctttttcga taacgtccca ttttgaggag
     8521 tcgatcataa tcggcacgcg agcgatatcc ggttcaccgg caatcagatt gagaaaacgc
     8581 accatcgccg cttcggcatc gagcatccct tcatccatgt tgatatcgat aatctgcgcg
     8641 ccgttttcca cctgctgacg cgccacatcc tgcgcctcgc tgtatttctc ttctttgatc
     8701 aggcgcttga acttagcaga gccggtgacg ttggtgcgtt cacccacgtt cacaaacagg
     8761 ctatcgtcgc caatgttcag cggctccagg ccagacaaac ggcaggctac gggaatttcc
     8821 ggcagtttgc gcggcgctaa tccttctact gcacgactca tcgctgcaat atgttgcggc
     8881 gtggtcccgc agcaaccacc gacgatattg agaaagcccg cttgcgccca ttcacgtatc
     8941 tgttttgcca tcgtgtcggc gtcgagatcg tattcaccaa aggcgttagg cagcccggcg
     9001 ttcgggtggg cggtgacgta gcattccgca atccgtgata gttcctgcac gtactggcgc
     9061 agttcatcgg gccccagcgc acagttcagg ccaaaggtta gcgcttcggc gtggcgcaat
     9121 gagttgtaaa atgcttcggt ggtctgcccg gagagcgtgc gcccggaggc gtcggtgatg
     9181 gtgccggaga tcataatcgg cagctcaacg cccagcgctt caaactccgt tttcaccgca
     9241 aaaaccgccg ctttggcgtt aagggtgtcg aaaacggttt caatcaggat cagatccgca
     9301 ccaccttcca ccagcgcttt ggtggattcg caataggccg ccaccagctg gtcaaaagtg
     9361 atgttgcgga atgccggatc gttgacgtcc ggagaaatag acgccgtgcg gttggttggg
     9421 ccgagaacac cggcaacgta gcgcggtttc tccggcgtgc gcgcggtcca ctcgtcagca
     9481 caggcgcgcg ccagtttcgc cgccgcaaag ttgatttccg ccgacaggga ttccatctgg
     9541 taatccgcca tcgcaatggt cgtggagttg aaggtgttgg tttcgatgat atccgcgccc
     9601 gcttcaaagt aggcgttgtg gatagcggcg atcacttccg gtttgctgag caccagcagg
     9661 tcgttgttgc ctttgagatc gcatggccag tcggcaaagc gttcaccacg aaaatcggct
     9721 tcgttcagtc gataactctg gatcatggtg cccataccgc cgtccagcac cagaatacgt
     9781 tcatttaact gcgcacgcag ttgttccact ttgctgctca cacttgctcc cgacacgctc
     9841 aacccagatt taataaaaat tcaacaaacc atactggcat aaacgcatct gtggtaaaag
     9901 cgaccaccac gcaacatgag atttgttcaa cattaactca tcggatcagt tcagcaacta
     9961 ttgcattagc taacaataaa aatgaaaatg attcccacga tgcagaaaaa ggagactgtc
    10021 atggtcgcac ccattcccgc gaaacgcggc agaaaacccg ccgttgccac cgcaccagcg
    10081 actggacagg ttcagtcttt aacgcgtggc ctgaaattac tggagtggat tgccgaatcc
    10141 aatggcagtg tggcactcac ggaactggcg caacaagccg ggttacccaa ttccacgacc
    10201 caccgcctgc taaccacaat gcaacagcag ggtttcgtgc gtcaggttgg cgaactggga
    10261 cactgggcaa tcggcgcaca tgcctttatg gtcggcagca gctttctcca gagtcgtaat
    10321 ttgttagcaa ttgttcaccc tatcctgcgc aatctaatgg aagagtctgg cgaaacggtc
    10381 aatatggagg tgcttgatca aagcgatcac gaagcgatta ttatcgacca ggtacagtgt
    10441 acgcatctga tgcgaatgtc cgcgcctatc ggcggtaaat tgccgatgca cgcttccggt
    10501 gcaggtaaag cctttttagc ccaactgagc gaagaacagg tgacgaagct gctgcaccgc
    10561 aaagggttac atgcctatac ccacgcaacg ctggtgtctc ctgtgcattt aaaagaagat
    10621 ctcgcccaaa cgcgcaaacg gggttattca tttgacgatg aggaacatgc gctggggcta
    10681 cgttgccttg cagcgtgtat tttcgatgag caccgtgaac cgtttgccgc aatttctatt
    10741 tccggaccga tttcacgtat taccgatgac cgcgtgaccg agtttggcgc aatggtgatt
    10801 aaagcggcga aggaagtgac ac
//
LOCUS       DAJVUJ010000143        10795 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.143, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000143 DAJVUJ010000000
VERSION     DAJVUJ010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10795
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.143"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            1..613
                     /locus_tag="OQQ48_003310"
                     /pseudo
     CDS             1..613
                     /locus_tag="OQQ48_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
     gene            610..1224
                     /locus_tag="OQQ48_003311"
     CDS             610..1224
                     /locus_tag="OQQ48_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HCS1953968.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEAARQDVAVTVLETET
                     GRYSDTLRSALVSLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(1281..2738)
                     /gene="pepD"
                     /locus_tag="OQQ48_003312"
     CDS             complement(1281..2738)
                     /gene="pepD"
                     /locus_tag="OQQ48_003312"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCS1953969.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            2999..3457
                     /gene="gpt"
                     /locus_tag="OQQ48_003313"
     CDS             2999..3457
                     /gene="gpt"
                     /locus_tag="OQQ48_003313"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCS1953970.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3549..4793
                     /gene="frsA"
                     /locus_tag="OQQ48_003314"
     CDS             3549..4793
                     /gene="frsA"
                     /locus_tag="OQQ48_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCS1953971.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIVMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            4851..5252
                     /gene="crl"
                     /locus_tag="OQQ48_003315"
     CDS             4851..5252
                     /gene="crl"
                     /locus_tag="OQQ48_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCS1953972.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(5291..6346)
                     /gene="phoE"
                     /locus_tag="OQQ48_003316"
     CDS             complement(5291..6346)
                     /gene="phoE"
                     /locus_tag="OQQ48_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HCS1953973.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGGEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAIGMTYQF"
     gene            6634..7737
                     /gene="proB"
                     /locus_tag="OQQ48_003317"
     CDS             6634..7737
                     /gene="proB"
                     /locus_tag="OQQ48_003317"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCS1953974.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            7749..9002
                     /gene="proA"
                     /locus_tag="OQQ48_003318"
     CDS             7749..9002
                     /gene="proA"
                     /locus_tag="OQQ48_003318"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HCS1953975.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTCDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            9117..9192
                     /locus_tag="OQQ48_003319"
     tRNA            9117..9192
                     /locus_tag="OQQ48_003319"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9150..9152,aa:Thr,seq:cgt)
     gene            complement(10247..10681)
                     /locus_tag="OQQ48_003320"
     CDS             complement(10247..10681)
                     /locus_tag="OQQ48_003320"
                     /inference="COORDINATES: protein motif:HMM:NF024139.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS1953976.1"
                     /translation="MGYDSRLDRLATTSWHSFFNNITARGEIMEPYSLTLDEACHFLK
                     ISRPTAINWIRTGRLQATRKDPTKNKSPYLTTRQACIAALQSPLHTVQVSAGDGITEE
                     RKCHSSAEVKYGTPVSHCRTVKDLNSLLEQRTKGRRQNSMTS"
     gene            complement(10597..>10795)
                     /locus_tag="OQQ48_003321"
     CDS             complement(10597..>10795)
                     /locus_tag="OQQ48_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1953977.1"
                     /translation="SLEVEPDERESYELFMEKHFGGLVDSRRAKNGDNEYMAWDMTLG
                     WIVWQQRAGIHFSTISQQEVK"
BASE COUNT         2566 a         2659 c         2786 g         2784 t
ORIGIN      
        1 agtctatttt acagcggcat attgcctcgt tctcgcatca tggtttgcct caaggcagtg
       61 acgacgcgct acggtatatt gcggaacatg acgatgcgct ggcgtttgcg tgtattaatc
      121 gccagatgat cgctttgcgc ataatgcaac aagttaaggc cacgggaagt ccggttctgg
      181 atgtggcgca taactttgtt agcgcgtgtc aaatcggcgt caacagggct ggttgcatcg
      241 taaaggtgcc acgccggatg acaacggtct ggtgattatt cccggttcac gtggtgatta
      301 ctcctggatt gtacagcctg ttgcgaacga aaaaacgctc cactcgctgg cgcatggggc
      361 agggcgtaaa tgggggcgca ccgagtgtaa atggcgtctg gcagcgaaat acacggtgac
      421 gcaactctcg cggactgaac ttggcagtcg gataatttgt cgcgataaac aactcatctt
      481 tgaagaagcg ccacaagcct ataaatcggc tgaaagcgtg gtgcaatgtc tggtgcaggc
      541 cggtttaatt attcctgtcg cgcggctgcg tccggtgcta acgctcaaaa acagtggagg
      601 gaaaaaagga tgatcttgct acaactctcc tcagcgcagg ggccggaaga atgttgtctc
      661 gcagtgaaaa aggcgctgga caggctaatt aaagaagctg cccgacaaga cgtcgcggta
      721 acggtgttgg aaacagaaac gggccgctac tctgacacgc tgcgttcagc tctggtttct
      781 ctggatggcg ataacgcatg ggcattaagc gaaagctggt gtggcactat tcagtggatt
      841 tgtccgagtc cgtatcggcc tcatcatggg cgcaaaaact ggtttctggg cattgggcgt
      901 tttaccgctg atgagcagga gcaatccgat gcgatccggt atgagacgct gcgttcgtcg
      961 gggccgggcg gtcaacatgt caataaaacc gactcggcgg tacgcgccac gcatttggca
     1021 tccggtatta gcgtgaaggt tcagtcagag cgtagtcagc atgctaacaa gcggctggca
     1081 cgattgctga ttgcctggaa gctggagcag cagcaacagg aaaatagcgc ggtgctgaaa
     1141 tcgcagcggc gaatgttcca tcaccagatt gaacgtggca acccgagacg gacatttaca
     1201 gggatggctt ttatcgaagg ataatgaagg atgaaactgc cggaaggcga ttaaacgcca
     1261 tccggcagca aatcaaataa ttacttcgcc ggaatttctt tcagcagttc agtcagcagt
     1321 gtccagtaat gacctacgct ttcgatgtga acttgctcat ccggagagtg tggaccggtg
     1381 atagttggcc cgatagaaac catgtccatt tccggatacg gttttttgaa cagaccacat
     1441 tccaggcccg cgtggataat ctggatgttc ggcgtcttgt tgaacaggcg ctgataggtt
     1501 tcacgtacca gatgcatcac cggagaatta gcgtccggct gccagccagg atatgcgcct
     1561 ttcgcttcgg ttttcgcgcc agccagttta cccagcgaat ccagcatgct caccacgtag
     1621 tctttaccgc tgtcgatcag tgaacggatc aggcagtgaa tttctacgtt attgtcagtc
     1681 atggtcacca caccgacgtt cagggaggtt tcaaccacac ctttggctac atcggagttg
     1741 cggatcacac cgttcggggt ggcgttcagc agacgaataa aggtatcgcg agatttcgca
     1801 atcagggcag ctttatcgtt cgctacagag tccagcaaca aggccagatt tttctctttc
     1861 tctgccagct cgtttttcag gatctcctga taggtattca ccagagattt cagtgcgtcg
     1921 actttatcag ctgcgacagc aatggtcgca aaggcttcac gcgggatggc gttacgcagt
     1981 gtgccgccgt tgaaatcgat aaggcgcagg tccagttctt ctgcatgacc cgccaggaag
     2041 cgcaccagca gtttgttggc attacccagg ccaacgtgga tttcaccgcc ggagtgaccg
     2101 cctttcagac cttttaaggt taacttgaag gtttcaaaac cagctggaac cgcttcacga
     2161 tctaaatgca ggttggaggt gaagtcgata ccccccgcac aacccatgta gatttcacct
     2221 tcttcttcgg agtcggtgtt aatcagaata tcagcctgca accagttgct ctgtaagcca
     2281 aaagcaccgt ccataccggc ttcttcggtc atggtcagca gcacttccag cggaccgtga
     2341 accacgtttt cgtcagccag aaccgccagt gcagaggcca taccaatgcc gttatccgca
     2401 cccagcgtgg tgccgcgcgc tttaacccat tcgccatcaa tataaggctg gataggatct
     2461 ttcgtgaagt catgcacggt gtcgttattt ttctgcggca ccatatcgag gtgggcctgt
     2521 aagacgaccg gtttacgatt ttccatacct gcggtagcag gtttacgaat caggatatta
     2581 cctacctgat cgcgttcgac atggaaacct ttctcttttg cccaaccaac aatgtattca
     2641 gcgagttgct cttcatgata ggacgggtgc ggaatagaac agattttggc aaaaatatcc
     2701 cacagcggct gtggagataa ttgagacagt tcagacacgt taagtctcct tgtcgatcac
     2761 ccgcaaaaca gtattgcagg tcacagggtt agcagaaaat gttgtcaaca caagacaggc
     2821 ttgcgagata tgtttgagaa taccacttta tcccgcgtca gggagaggca gtgcgtaaaa
     2881 agacgcggac tcatgtgaaa tactggtttt tagtgcgcca gatctctata atctcgcgca
     2941 acctattttc ccctcgaaca ctttttaagc cgtagataaa caggctggga cacttcacat
     3001 gagcgaaaaa tacatcgtca cctgggacat gttgcagatc catgcacgta aactcgcaag
     3061 ccgactgatg ccttctgaac aatggaaagg cattattgcc gtaagccgtg gcggtctggt
     3121 accgggtgcg ttactggcgc gtgaactggg tattcgtcat gtcgataccg tttgtatttc
     3181 cagctacgat cacgacaacc agcgagagct taaagtgctg aaacgcgcag aaggcgatgg
     3241 cgaaggcttc atagttattg atgacctggt ggataccggt ggtactgctg ttgcgattcg
     3301 tgaaatgtat ccaaaagcgc actttgtcac catcttcgca aaaccggctg gtcgtccgct
     3361 ggttgatgac tatgttgttg atattccgca agatacctgg atcgaacagc cgtgggatat
     3421 gggcgtcgta ttcgtcccgc caatctccgg tcgctaatct tttcaacgcc tggcactgcc
     3481 gggcgttgtt ctttttaact tcaggcaggt tacaatagtt tccagtaagt attctggagg
     3541 ctgcatccat gacacaggca aacctgagcg aaaccctgtt caaaccccgc tttaaacatc
     3601 ctgaaacctc gacgctagtc cgccgcttta atcacggcgc acaaccgcct gtgcagtcgg
     3661 ctcttgatgg taaaaccatc cctcactggt atcgcatgat taaccgtctg atgtggatct
     3721 ggcgcggtat tgacccacgt gaaatcctcg acgtccaggc acgtattgtg atgagcgatg
     3781 ctgaacgtac cgacgatgat ttatacgata ctgtgattgg ctaccgtggt ggcaactgga
     3841 tttatgagtg ggccacccag gcgatggtgt ggcaacaaaa agcctgtgcg gaagaagatc
     3901 cgcaactcag tggtcgacac tggctgcatg cggctacgtt gtacaacatt gccgcctatc
     3961 ctcatctgaa aggagatgac ctggccgagc aagcgcaggc tttatcaaac cgcgcctatg
     4021 aagaggccgc ccagcgtctg ccgggcacga tgcggcagat ggagtttacc gtacccggcg
     4081 gtgcgcccat caccggcttt ttgcatatgc cgaaaggcga tggcccgttc ccgacagtat
     4141 taatgtgtgg tggtctggat gcgatgcaga cggactatta cagcctgtat gaacgttatt
     4201 ttgcgccgcg cggcattgtg atgctgacta ttgatatgcc gtcggtgggc ttttcttcaa
     4261 aatggaagct cacccaggac tccagcctgt tgcatcagca cgtcttaaag gcgctgccta
     4321 acgtaccgtg ggtggatcac actcgcgtcg cggcctttgg tttccgtttc ggcgctaacg
     4381 ttgccgtgcg tctggcatat cttgaatcgc cgcgtctgaa agcggttgcc tgtcttggtc
     4441 cggtagttca taccctgttg agtgatttta agtgccagca acaggtgccg gaaatgtatc
     4501 ttgacgttct ggcgagtcgt ttggggatgc atgatgcttc cgacgaagcg ttacgcgtgg
     4561 agctgaatcg ctattcatta aaagtgcaag gattgctggg acgtcgctgc ccaacgccaa
     4621 tgttatcagg ctactggaag aacgatccgt tcagcccgga agaggactca cgcttaatca
     4681 cctcatcatc tgctgacggt aaattattag agatcccatt taacccggtg tatcggaatt
     4741 ttgacaaagg tcttcaggaa atcaccggct ggatcgaaaa acgcttgtgt taaaaatttg
     4801 ctaaattttg ccaatttggt aaaacagttg catcacaaca ggagatagca atgacgttac
     4861 cgagtggaca cccgaagagc agattgatca aaaaatttac cgcactaggc ccgtatattc
     4921 gtgaaggtaa gtgcgaagat aatcgattct ttttcgattg tctggctgta tgcgtcaacg
     4981 tgaaaccggc accggaagtg cgtgaattct ggggctggtg gatggagctg gaagcgcagg
     5041 aatcccgttt tacctacagc taccagtttg gtctgttcga taaagcaggc gactggaaga
     5101 gtgttccggt aaaagatact gaagtggttg aacgactgga gcacaccctg cgtgagtttc
     5161 acgagaagct gcgtgaactg ctgacgacgc tgaatctgaa gctggaaccg gcggatgatt
     5221 ttcgtgacga gccggtgaag ttaacggcgt gagtgaaatg tgccggatgc atcacatccg
     5281 gcaatatcta ttaaaactga tacgtcatcc caatcgcgac aatatcatca ttattaatat
     5341 tcaatttgtt atcgctatcc agttggttga ttttataatc aacaaatgct gacatatttt
     5401 tgttgaaata atacgtagca ccgacgtcga tataattaac cagatcttca ccaccgatgc
     5461 cctcaatatc tttccctttc gataagacat aacccagcga tggacgcaga ccaaagtcaa
     5521 actggtattg agcgaccgct tcaaagttct gtgtcttatt ggcaaagccg ccagttattg
     5581 gcgtcatttt gcgagtttca gaatagaaag ttgccagata aatattattg gcatcgtatt
     5641 tcagacctgt tgcccatgct tctgcacgct tgcctgtgcc acggctttgc aggttctgct
     5701 cgttggtgcg atctgagttg gtataggccc cactaatggc gaaatcgctg ccgccaaagt
     5761 catatgtcaa tgacgtgccg aagccatcgc cgttttgctt tttaacgtcg cggttttcgt
     5821 ttttcccttg atattgcagg gttaagttca ggccatcgat aacgccgaag aagtcggtgt
     5881 tccgatacgt cgccagaccg ctggcgcgtt tggtcataaa gttgtcggtc tgcgccgagg
     5941 agtcgccacc aaattccgga aacatatcgg tccaggcttc cacgtcatac aacgcgccca
     6001 ggttacgacc ataatcgaaa gaacccaaat ctttatattt caacccggca aaagcgagac
     6061 gcgttttttg ctgtgcagta tcactctctg ctttattacc ggcaaactct gcttcccaac
     6121 gaccataacc agtcagttga tcgttaattt gtgtttcgcc tttaaaacca aaacggatat
     6181 aactctggtc gccatcttta ctgtcgttat cactcatata atgcatggct ttaactttgc
     6241 catagacatc cagtttatta ccgtctttgt tatatatttc tgcggcctgt acggatgcag
     6301 atgccacaat gcccatcacc actaatgcca gagtgctctt tttcattttc attcctgatt
     6361 ttaattaacg cgcgaatatt cagcgggaga gccccgttga aaacaggaaa gtttttaacc
     6421 tgagattgtt aaagatatat tacagattaa taatattctt aaaatgtggt aatttattaa
     6481 atctgtaata aaagcgtaaa caactgccgc taggcttgct gatcccgcgc aacaaaacgc
     6541 catgctttgc tcgcagatgg ttggcaaccg acgacagtcc tgctaaaacg ttcgtttgat
     6601 atcatttttc ctaaaattga atggcagaga atcatgagtg acagccagac gctggtggta
     6661 aaactcggca ccagtgtgct aacaggcgga tcgcgccgtc tgaaccgtgc ccatatcgtt
     6721 gaacttgttc gccagtgcgc gcagttacat gccgccgggc atcggattgt tattgtgacg
     6781 tcgggcgcga tcgccgccgg acgtgagcac ttgggttacc cggaactgcc agcgactatc
     6841 gcctcgaaac aactgctggc agcggtaggg cagagtcgac tgattcaact gtgggaacag
     6901 ctgttttcga tttatggcat tcacgtcggg caaatgctgc tgacccgtgc tgatatggaa
     6961 gaccgtgaac gcttcctgaa cgcccgcgac accctgcgtg cgttgctcga taacaatatc
     7021 gttccggtaa tcaatgagaa cgatgctgtc gctacggcag agattaaagt cggcgataac
     7081 gataaccttt ctgcgctggc ggcgattctg gcgggtgccg ataaactgtt gctgctgacc
     7141 gatcaaaaag gtttgtatac cgctgacccg cgcagcaatc cgcaggcaga actgattaaa
     7201 gatgtttacg gcattgatga cgcactgcgc gcgattgccg gtgacagcgt ttcaggcctc
     7261 ggaactggcg gcatgagtac caaattgcag gccgctgacg tggcttgccg tgcgggtatc
     7321 gacaccatta ttgccgcggg cagcaagccg ggcgttattg gtgatgtgat ggaaggcatt
     7381 tccgtcggta cgctgttcca tgcccaggcg actccgcttg aaaaccgtaa acgctggatt
     7441 ttcggtgcgc cgccggcggg tgaaatcacg gtagatgaag gggcaactgc cgccattctg
     7501 gaacgcggca gctccctgtt gccgaaaggc attaaaagcg tgactggcaa tttctcgcgt
     7561 ggtgaagtca tccgcatttg caacctcgaa ggtcgcgata tcgcccacgg tgtcagtcgt
     7621 tacaacagcg atgcattacg ccgtattgcc ggacaccact cgcaagaaat tgatgcaata
     7681 ctgggatatg aatacggccc ggttgccgtt caccgtgatg acatgattac ccgttaagga
     7741 gctggccgat gctggaacaa atgggcattg ccgcgaagca agcctcgtat aaattagcgc
     7801 aactctccag ccgcgaaaaa aatcgcgtgc tggaaaaaat cgccgatgaa ctggaagcac
     7861 aaagcgaaat cattctcaac gctaacgccc aggatgttgc tgacgcgcga gccaatggcc
     7921 ttagcgaagc gatgcttgac cgtctggcac tgacgcccgc acggctgaaa ggcattgccg
     7981 acgatgtacg tcaggtgtgc aacctcgccg atccggtggg gcaggtaatc gatggcggcg
     8041 tactggacag cggcctgcgt cttgagcgtc gtcgcgtacc gctgggagtt attggcgtga
     8101 tttatgaagc gcgcccgaac gtgacggttg atgtcgcttc gctgtgcctg aaaaccggta
     8161 atgcggtgat cctgcgcggt ggcaaagaaa cgtgtcgcac taacgctgca acggtggcgg
     8221 tgattcagga cgccctgaaa tcctgcggct taccggcggg tgccgtgcag gcgattgata
     8281 atcctgaccg tgcgctggtc agtgaaatgc tgcgtatgga taaatacatc gacatgctga
     8341 tcccgcgtgg tggcgctggt ttgcataaac tgtgccgtga acagtcgaca atcccggtga
     8401 tcacaggtgg tataggcgta tgccatattt acgttgatga aagtgtagag atcgctgaag
     8461 cattaaaagt gatcgtcaac gcgaaaactc agcgtccgag cacatgtaat acggttgaaa
     8521 cgttgctggt gaataaaaac atcgccgata gcttcctgcc cgcattaagc aaacaaatgg
     8581 cggaaagcgg cgtgacatta cacgcagatg cagctgcact ggcgcagttg caggcaggcc
     8641 ctgcgaaggt ggttgctgtt aaagccgaag agtatgacga tgagtttctg tcattagatt
     8701 tgaacgtcaa aatcgtcagc gatcttgacg atgccatcgc ccatattcgt gaacacggca
     8761 cacaacactc cgatgcgatc ctgacctgcg atatgcgcaa cgcccagcgt tttgttaacg
     8821 aagtggattc gtccgctgtt tacgttaacg cctctacgcg ttttaccgac ggcggccagt
     8881 ttggtctggg tgcggaagtg gcggtaagca cacaaaaact ccacgcgcgt ggcccaatgg
     8941 ggctggaagc actgaccact tacaagtgga tcggcattgg tgattacacc attcgtgcgt
     9001 aaataaaacc gggtgatgca aaagtagcca tttgattcac aaggccattg acgcatcgcc
     9061 cggttagttt taaccttgtc caccgtgatt cacgttcgtg aacatgtcct ttcagggccg
     9121 atatagctca gttggtagag cagcgcattc gtaatgcgaa ggtcgtaggt tcgactccta
     9181 ttatcggcac catttaaatc aataagttac acatcattag tacgttcctt atttcttgac
     9241 tgggacaaat ttgggaccga tgggttcaga atcgagtcta tttgccgtgc gtgttcggta
     9301 aggtgattag gtgcgaggtg agcatatcga cgaaccattt cgatagactc ccagcctccc
     9361 atttcctgta acactgataa cgggactccg gcttgaacca gccaacttgc ccaggtgtgt
     9421 ctcaagtcgt gaaatctgaa atcatcaata cctgctcgtc tcagcgccgc tttccaggct
     9481 gtgtttgcgt cataccgcat cttcctgact gttggcgctt tcgttccgtc tggtttggta
     9541 cagctttcct tatacacaaa tacccaacgg tgatgattcc cgatttgttt tttcaatacg
     9601 cgacatgcag tatcattcag cgcaacgcca attgcgcggt ttgatttact ctcttccggg
     9661 tttatccatg ccacccggcg ctgcatgtct atttgttgcc attcaaggtt gatgatgttc
     9721 gagcgtctta agcctgttgc aagtgcaaat tcaacaacag actttaatgg ctccggacat
     9781 tcatcaatca gcctttgtgc ttcatgaggc tccagccagc ggatccgttt attctttggt
     9841 tgaggcactt taataattgg tgccttatcc agcattttcc attcacgctc tgcggctctt
     9901 agcagggcct ttataaatga aagatgcgta gccttcgttg caacggacgc tggttttggc
     9961 gtgtattctg gaacaggttt cccttttttt ctgcatgctt ctgccctgag tttccagttt
    10021 tcctcatgac gccggttcgt cattttctgc attgctgaat aaatttttga ttcagtaatg
    10081 tctcttagtt gcattcctgc gaaatgttga agccagaatc cgatccggct tttgtcatcg
    10141 tccagtgatt ttttatgtgc tttctcttca agccacctga cacacgcttc ctcgaacgtt
    10201 atatcaggaa tttcaccaag tttgctgacc cgccatgctt cagcctttag cttgtcatgg
    10261 agttctgtcg cctgcctttt gtcctttgtt ccaagagact gtttaaatct tttaccgttc
    10321 ggcaatgtga aactggcgta ccatatttca cctctgcgga agagtgacat tttctttcct
    10381 ctgttatgcc atcacccgcg ctcacctgga cagtatgcag cggagactga agcgccgcaa
    10441 tgcaggcttg tcgtgttgtg aggtaaggag atttattctt agtgggatct ttgcgtgttg
    10501 cctgcagacg ccctgtgcgt atccagttaa tggcagtcgg tctggatatc ttgagaaaat
    10561 gacaggcctc atctagcgtg aggctgtatg gttccattat ttcacctctt gctgtgatat
    10621 tgttgaaaaa tgaatgccag ctcgttgttg ccagacgatc caaccgagag tcatatccca
    10681 tgccatgtat tcgttatcgc cgttttttgc tctccgacta tctactaagc caccgaaatg
    10741 tttttccata aataattcat aggattcgcg ttcatctggc tctacttcca gagat
//
LOCUS       DAJVUJ010000144        10698 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.144, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000144 DAJVUJ010000000
VERSION     DAJVUJ010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10698
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.144"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(219..2078)
                     /gene="gss"
                     /locus_tag="OQQ48_003322"
     CDS             complement(219..2078)
                     /gene="gss"
                     /locus_tag="OQQ48_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HCS1953978.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            2283..3149
                     /gene="yghU"
                     /locus_tag="OQQ48_003323"
     CDS             2283..3149
                     /gene="yghU"
                     /locus_tag="OQQ48_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HCS1953979.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPICVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(3272..3520)
                     /gene="hybG"
                     /locus_tag="OQQ48_003324"
     CDS             complement(3272..3520)
                     /gene="hybG"
                     /locus_tag="OQQ48_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HCS1953980.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3533..3874)
                     /gene="hypA"
                     /locus_tag="OQQ48_003325"
     CDS             complement(3533..3874)
                     /gene="hypA"
                     /locus_tag="OQQ48_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCS1953981.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3867..4355)
                     /gene="hybE"
                     /locus_tag="OQQ48_003326"
     CDS             complement(3867..4355)
                     /gene="hybE"
                     /locus_tag="OQQ48_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HCS1953982.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(4348..4842)
                     /locus_tag="OQQ48_003327"
     CDS             complement(4348..4842)
                     /locus_tag="OQQ48_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HCS1953983.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(4842..6545)
                     /gene="hybC"
                     /locus_tag="OQQ48_003328"
     CDS             complement(4842..6545)
                     /gene="hybC"
                     /locus_tag="OQQ48_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HCS1953984.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6542..7720)
                     /gene="hybB"
                     /locus_tag="OQQ48_003329"
     CDS             complement(6542..7720)
                     /gene="hybB"
                     /locus_tag="OQQ48_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HCS1953985.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLKRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTREELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7710..8696)
                     /gene="hybA"
                     /locus_tag="OQQ48_003330"
     CDS             complement(7710..8696)
                     /gene="hybA"
                     /locus_tag="OQQ48_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HCS1953986.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8699..9817)
                     /gene="hybO"
                     /locus_tag="OQQ48_003331"
     CDS             complement(8699..9817)
                     /gene="hybO"
                     /locus_tag="OQQ48_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HCS1953987.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(10006..10293)
                     /locus_tag="OQQ48_003332"
     CDS             complement(10006..10293)
                     /locus_tag="OQQ48_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HCS1953988.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10412..>10698)
                     /locus_tag="OQQ48_003333"
     CDS             complement(10412..>10698)
                     /locus_tag="OQQ48_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCS1953989.1"
                     /translation="LACAVPFYGRQAPTADVAKIEAPLLLHFAELDTRINEGWPAYEA
                     ALKANNKVYEAYIYPGVNHGFHNDSTPRYDKSAADLAWQRTLKWFDKYLS"
BASE COUNT         2607 a         2930 c         2652 g         2509 t
ORIGIN      
        1 atatcatttt ttaacgataa aaagagtgtt atttcttgtg tatcagacaa taagcattca
       61 ttcaaaacct ttagaaataa tcaaaacaca ggtattccac tggttaatat attcacatat
      121 gaaatgaata aatattagaa catataaata ttgaatattt tgattaatcc ccgtactgat
      181 tattcttcat aatcagtacg gggttgctac aacatgtatt actttttcac cacaattaac
      241 ggttcaatat cactctcttt tttgatgacc agtgattcat caccgcgcaa acacgtccca
      301 ccgtagttgc cgcccacggt gaaggtacat acctgaatgt atttaccgtc cactttcggc
      361 aaacaccaca gttgctgata gatgtttttc tgctcggcaa atttaccgct ggttttgtcc
      421 agcacctctt catgatggct gaccaggtcg atattgctac cacagcgacc ggcaatcggt
      481 ttcactgcgt aacctgtttt caccaattca tcattaacag tgaaatcggt atccagcagg
      541 taacgatggt gcgggaacag cgaccagagg atcggcagaa tcgctttgtt gccggggatc
      601 accgtccaca gcggctcaaa gactagcact tccgggcgca gcaatacgtc gataagacgc
      661 acttcgtttt gcggatgacc ggtacggatt ggcaccgcag caaactcacg gtcgctaact
      721 tcacgaatct gatcaaacgc ggtttcccac gcccaggttt tccacacgca gttaaccagt
      781 cgcccttccc catcaatcag ttgcccggca gcatcccagc ccagttcatc caatccacgc
      841 aagatacgcg tttcaaagcc cgcctggtgc agcgcctgct ccataaactg cgcgtgatag
      901 ttttcctcga tatctttgtc ctgcatgata tggacaaacg gacgtgcacg actgtgtttc
      961 caggcaccag ccaattcgtt aatcagccct tccgccggat tgaagccgtt gcctttatag
     1021 ccctgctccg cccaacgttc gaggatcaag cccgcttcgg tatgacagga ggcggagtcg
     1081 gcgttgtact cgtaaacctt caggccacgc tcatccatgc agaaatccat acgaccagtg
     1141 atcatatggt gacggcgacg ctgccaggag agacgcaaac gtggccagag gattttcgga
     1201 atgtcgaaca gcgccagcag gttgtcatct ttcagtacct tgtcggttgc gtgaagatac
     1261 atcaggtgca gctcgttggt ggctttaatt agctcctgct cggcactctc ggtaatggtg
     1321 tagtaatgat aaggatcctg attgatcacc tgaccgttgg cctgcacata ggcgttttgc
     1381 agcggatctt tttcatccag ccatttaccg tcaaactggc ctttgttttc caggcgcgct
     1441 ccgctgattt tcagcagctc gcctgcaatt tccggctgcg gtaagctgta ttcagtatct
     1501 tccgtctgga tcatccagcc cagaatggtg gtgtcatcaa aagtgtcttt cagggtatag
     1561 cagccgtttt cgaccaccat ctccagctca cgcgtccact gttgcccttg cggcaacggg
     1621 gaatgaatca cgttctgttc cgcaatacgg actttgttgc catgcaattg ggtaatgatg
     1681 gcgacatggc cagtgtcttt aaattcaccg cctttatccc agataagaag cgcacccgcg
     1741 accggcgcac gcggcgagcc gttaggaaat gcctgcaatg gcaggatgtt gtcattaacc
     1801 acttctcgca ggaagcgcag cgagaaaatc tcccacgcca tacccacgtc agtaaagacc
     1861 acaccgtaat tcagaaagag aaaacggcga gcaaattcaa cgcattgcca cttgtggccc
     1921 atatattcgt cgtcgatata gctacggaat acggcgtcat cttcgtattc ctgcggatcg
     1981 agagaactgt aatctgaaga gtagattgct accccacctg gggcgtagcc caataatgtc
     2041 ccgaacgggg catcctggct ggtcgttcct ttgctcatca ctttaccttt aacaatacaa
     2101 cctaagcagt tggcgtaatt gttgtgctct gattacctgc tcagcgacat taaccggaca
     2161 gaggtcagac taatcataca cctcatcgca gcggctggcg caaagggtta aaaaattcac
     2221 attctgtaca gcaagtgacc tgccacactg cttcaacact accactcaga aggcaactca
     2281 ctatgacaga caatacttat cagcccgcga aagtctggac gtgggataaa tccgctggcg
     2341 gcgcgttcgc caatattaat cgcccggttt ctggtccgac gcatgaaaaa acgctgcccg
     2401 ttggcaaaca cccattgcaa ctttattcgc tgggaacgcc gaacggtcag aaagtaacga
     2461 ttatgcttga ggagctgctg gcgctgggcg ttactggtgc agagtacgac gcctggctga
     2521 ttcgtattgg cgatggcgat caattctcca gcggctttgt cgaagtgaac ccaaactcga
     2581 agatcccggc gctgcgcgat catacgcata atccgccgat ctgtgtgttt gaatctggtt
     2641 cgatcctgct ttatctggcg gagaaatttg gctacttcct gccgcaggat ttggcaaagc
     2701 gtactgaaac gatgaactgg ctgttctggt tacagggcgc ggcaccgttc ctcggcggtg
     2761 gttttggtca cttttaccat tacgcaccgg taaagattga gtacgccatc aaccgcttta
     2821 ccatggaagc caaacgtctg ctcgacgtgc tggataagca actggcgcag cataagtttg
     2881 ttgcgggcga tgagtacacc attgcggata tggcgatttg gccgtggttt ggcaacgtgg
     2941 tgttaggtgg tgtgtatgat gccgctgagt ttcttgatgc gggcagttat aagcatgtac
     3001 aacgctgggc gaaagaagta ggcgaacgtc cggcggtgaa acgtgggcgt attgttaacc
     3061 gcaccaacgg accgctgaat gagcagttgc atgagcgcca tgacgccagt gatttcgaga
     3121 cgaatacgga agataagcgt caggggtaag ggttggtgtt cgtcgcagca agccatccag
     3181 gccggataag gcgttcacgc cgcatccggc aatcgtgcac aatgcctgat gcgacgctgc
     3241 cgcgtcttat caggcctacg aaagctaatc atcacgcgct ggtaatgtcg taatccattt
     3301 ggcgcagtgc gtctaatgtg gctttggctt catcttcgtc gatgatgctc atggcaaatc
     3361 cgacgtgtac cagcacccac tggcccagta gatcggcagg gttaccttca caaatcaggg
     3421 cgatattcac gtcgcgcttg ataccacata cttcaacctg cgcaagctgg tgaatatctt
     3481 caccgacagc cagcacctgg cctggaacgc caatacacat tattaactcc ggttattcaa
     3541 cttcaatact tttgacgatc agcgaatcgc cggtatcgac acgcaaccgc tcgccgtgac
     3601 agagcggaca ctgcgcatcg tgctgatgaa tctccaccac ctggctgcaa tcccagcacc
     3661 aggcctgggc gggtttatag acgatatgta aatcgcaccc ttgagccacc gttccgtggc
     3721 agacaatttc aaaactaaaa cggacggcgc tctcctcaac gcaggagagc gcgccaattt
     3781 ccagccacac ggcggtgacg cgcttaacat cgtgctgctc cgcctgccgt tggataattt
     3841 caacggcgct ctggcaaagc gacaactcat gcattttcgc cactcctgcg accaaacagc
     3901 agggcgcgac gccctgcgtg tggtacatcc ggattcgtga ctggcagcga aaggatcatt
     3961 cgtgcgcagt catccgtcag gcgttggccc tcttcaatcg acatactgtg cgaaagcggt
     4021 gacatcagcg aacaggagag gtattgcgaa acaccgtcca gttcgccaac ggtaaaggtc
     4081 atggtgccat acggcagttg cagaccaatt ttttcactca ctttgcgcag cggccagagt
     4141 tgatccgggc cggggaagat aactgcactc agcatccacg gggtgatcac acaccccgtc
     4201 cactgacctt cgaacagcgt aaaatcagaa acatacaccg gcattgaagg atgcagaaaa
     4261 gagagatcgt gcatcgaacg ccgggcaatt tcttcaaacg ctgcctgtac ttgcgccttc
     4321 ggggaggtct ggaaacctgc tatctcttca gtcatgaatc gcctcccgtg ggatggcttc
     4381 cacgcctgat tcgcgcagcg cagccagaac ctgctcaagc gcaggttcaa tcattgcttc
     4441 aaccgtcggc gttaagccga tgtgtggctc cagcgattcc gggatcacgc cgaccagggt
     4501 cagctttttc ggaaactcac cggtgaagcg cagggccgac aatacgtcgg ccaggccaag
     4561 ctgatgcgga gagattttgt tggtaaacaa cgccggaact tcttcatccc gcaggatcat
     4621 catcgttccc ggcgtgttct ttttcgacac aatggcatcc gcaataatca gatgatcgcg
     4681 atttgccatg tcgccaagca gctccattcc cgccgtgccg ccatcgagga tctcaacata
     4741 atccggcaga atgtatcgtt gctctaacgc ttcgacaatc cgcacaccga tggcttcatc
     4801 ggtcagcaaa atattgccga cccctaagac taaaatacgc attacagaac cttcactgaa
     4861 accacttcgt tgccgtcggc atccactacg tgtaccgcac aggccatgca cgggtcgaag
     4921 gagtgaatgg tacgcaccac ttccagcggt ttattcggat cagcaaccgg tgtacccacc
     4981 agcgactgct cgtaaggacc gacgtcatca ttgaagttac gcggaccaga gttccaggtt
     5041 gatggaacaa ctgcctggta gttactgata ataccgtctt taataaccat ccagtgagag
     5101 agcataccgc gcggcgcttc gaggaagcca acacctttga actcacccgt tgccggaatg
     5161 ttcggtttca caaaggtggt gtgatcgcct ttgccgatat tggtgatcag tgcactgtat
     5221 tgattttgca ggatatcctg caattcgcag cagtgaacgg tacgaccaat aatacggccc
     5281 agcgtggagt gcagctgcgc cacttccagc gtgttgccag tcagtttctg ataaatcgca
     5341 acgatttcat tcagtttgtt ctgggtagat tcgcgacctg ccgccagttt caccagcata
     5401 ttagccagcg gacccacttc taccgttttg ccgtagaaag tcggtgattt cacccaggaa
     5461 tatttcccgt cgtcagacca accatcataa gccggaatgg tggtaccttc ccacggtgcc
     5521 tgcggcgctt cgtctttata ccaggagtgc ttcgcgcttt cctgaatgcc tttgatcagg
     5581 tattcatcgg aatgagaagt gatcggacga tacgaggaca gatccgcatt ctcaatgtag
     5641 ccgcccggga acaggaagct gccgttttta ctgtcggtcg ggaattccgg cacgctcagg
     5701 tagttcaccg cacctttacc gcgcgtcagc cattccgggt agaacgcggc aataactgcg
     5761 gtatcaacct tataaacctg ttcaacaaag tcgctcagct tgtcgatgaa agacttgatg
     5821 tacatcaggc gctcaaggtt cagcacgccc aaaccgtcga ggttgatcgg gttcgcgaca
     5881 ccacctaccg ccaggttctg aatgtgcggc gttttaccgc ccagcagcgc cacgacgcgg
     5941 ttagcgtcac gctggcactc cagcgcctgc aggtagtgcg ctaccgcaat caggttcact
     6001 tccggcggca gtttcatcgc cgggtgaccc cagtagccat tggcgaaaat acccaactga
     6061 ccgctggcaa ccagatcttt gatcttgttc tgaactttag tgaactcttc cggactgttc
     6121 agatgccagg tcgaaacgcc tttcagcatt tcggaggctt tggttgggtc agcttgcagt
     6181 gcagaagtga tgtccaccca gtccagcgcc gaaagctgat agaaatgaac gatatggtca
     6241 tgcgtggtgt gcgcagccag aatgatgtta cggatgtatt gcgcgttaac cggaacgtcg
     6301 atattcagcg cactttctgc cgcacgaacg gaagacagcg cgtgagtggt agtacatacg
     6361 ccacagatac gttgcacaat catccatgca tcgcgcggat cgcggttttt cacgatctct
     6421 tccatgccgc gccacatggt accggaagcc catgctttcg aaacgacgcc attttcgatt
     6481 tcgcaatcga tgcgtaaatg cccctcaata cgggttaccg gatcaatagt aattctctgg
     6541 ctcatgcttt gctcgcctca tgacgattat gatcgttttg ttttaaagga ggaagtatcg
     6601 gcagtagacg aatgagtacg atgtaagcgc aaatctcaat agccacaaaa ccaatagaaa
     6661 tcaacagttc ttccctggtc gggaagtagg cgtaaccgcc gcccgggttg aatgccacca
     6721 gcgaataggt cagacgccag gttgcacaac ctaacagtgc gctcagtgct gacaggaaca
     6781 gcatgcggga atcattacgc agcttcgcca cacgcagaac gaccagcggg aagagcatca
     6841 gcaggacttc aatccagaac atcacggagt agaagtcacc ggcaaacgct aacgacagct
     6901 tgtcgcgata gataagctcg ccaaagcgca gcacgataaa aatcgccagc aacacactga
     6961 tggtgttggt cagcttgacg aacagactct tttcatccgg accgttgcca cgcagacccg
     7021 cctgcaccag cgaaccttca aagatgacaa tcgagaagcc catgatgaac gccgtcagca
     7081 gcgagaacag cggcaacatt tcatagctct gccacaacgg atgcaccttg tagcccgccg
     7141 agatcatcag tgaccccatt gaagactggt gcatggtcgg caacagcgcg ccaagtgcaa
     7201 tgatgaagaa catcaccttg ttcagacgct tgagagaaac cttccagccc agacgttcaa
     7261 acagtgccgg agcaaactcc agtgccatca cgccgatata gatggtcata cagaccgccg
     7321 tctcgaacag taccgagttc acgttgaagt gacccggaat gtagaagtac ggcaggttcc
     7381 agtagcgacc cacgtcgata gtgatcgaca agccacccag tgagtaacca aacagactcg
     7441 ccaacagcgc cggacgcacc agcggatggt attgcccacg gttaaagacg tataccgccc
     7501 acgccagcgc ccagccgcca caggcaaagc cggtaccaat cagcaggtca aacgcgatcc
     7561 acacgcccca cgggaagccg ccgttcaggt cagagacaga gcccagaccg aacaccagac
     7621 gcttcacaat caggagcata cagatgacga ttaacggtcc aaaaatcatg accggtttac
     7681 tgatgatttt gccgcccagc ggttgtggat catgactcat gatcgtctcc tccgtcgtga
     7741 tggtcgtttt tggtgttgcg acgaaccagc acggttaagc ccgccagcac agccagtggt
     7801 agcatcatgc ctttatacag ggtgtgttga atattttcgg aacgcgcacc ggtagaaaga
     7861 tcgtccagtt tcggcaggtc gagattttca taaggcacac ccgtcagtac cagaacctga
     7921 gtaccgccgc cctctttctc gccgtacaga tgcggataat atttcggcac cgtatgcagg
     7981 taagtgtcgc cagatttcag cgtctgacgc ggatagtggt attcgctgcc aggcttcagc
     8041 gccagacgtt ttttcgcctc cgccatcagc tcttcacgcg taccgaaaat caccgcgccc
     8101 gccgggcaca cttctacgca gccaggcaga ccgcctttat cgagacgttc cacacctttc
     8161 tggttgcaca gctcgcactt atgcagcgca ccaaacgggt tgttgtagtc gtacttcggc
     8221 acgttgtacg gacaggcgac catgcagtaa cggcaaccgg tgcacacgtc tttgtcgtaa
     8281 tggacaatgc cggttttcgg atcttttttc agtgcagaga ccgggcacac agagacacag
     8341 ttcggatcga cgcagtgcat acactgtttc ttaatgtacg cgtagccgtt ctcctcctgg
     8401 tctttgttga cccctgtgcc gctggtccac acctgaatga tgttattggt atacggcgac
     8461 agtttgtcgt tgttcgacca ggtctgttcc ccttgcgggt tacgttcagg gaagttgata
     8521 tcctgacact tggtgacgca agcctggcag cctacgcaca aggtcgagtc gtacaacatc
     8581 cccagcgatc ccggaattgg cgggcggttt tcagcagccg catgactgac agacggcagc
     8641 gcgcccgtca gcaatgcccc gcaggaggct gctttaataa aattacgtct gttcacggtt
     8701 attctccccg tgagtcagcg ttatctttct tttgctgacg acccagttca cgcaccgcca
     8761 tcacgctgac accggcaacc agcccaacca caccgccgag caaaccaata gcgcctgcag
     8821 agacgttacc gccctcttta gcgttaacat ccggtttctg tgaacgcgga gtctgatttt
     8881 cgacgttggc aagctgatgg atgcctttat ggaagccgat accttcttcg ttacagccat
     8941 agcatgggtg accaatcgct accggccaca cgccgccaac atcgcagaat tgcagcgttg
     9001 agcagttgcc gtaagtttct ggccctttac agccgaggtg gtacaggcac cagccttcac
     9061 ggtggccttc atcaccgaac tctttggcaa aacgaccagc atcgaagtgc gggcgacgtt
     9121 cgcagtgttc gtgaatcaga cggccatagg cgaaggtcgg acgatttttg tcatccagtt
     9181 tcggcggttt gccgtaagtg atgatgtgcg caacggtcgc gaggaagttg tgcgggttcg
     9241 gcgggcagcc cggaatattg ataacggttt tgcctggcag aacttcttgc aggctgactg
     9301 cgccagttgg gttaactcca gctgcggcaa caccgcccca cgcagagcag gaaccgatag
     9361 cgataatcgc tgctgcgcct tctgccgctt tgcggatgtg atccacaatc ggctcaccag
     9421 caaccatgca ataaataccg ttatctttta atgggatgga accatccaca accaacacat
     9481 actgcccttt gtacttctca agtgcgttat gtttgttctc ttcgacctga tgaccgaagg
     9541 cggcggaaag cacttcgtga tactccagag agatagtctc cagcacgagg ttttctaccg
     9601 ttggatgcgt tgcacgaagc agagattccg tacaaccggt gcactcctgc gcgccaatcc
     9661 agataactgg cggacgctgc gggttagtaa ccgattcggc catctctgca gcggctttgc
     9721 tacttaaccc catggtggcg gctaatgctg cacaaagctt catgaaatca cgacggttaa
     9781 tgccgtgaga atggatgagg gtgttatctc cagtcattta tagttattcc gttgcgaaga
     9841 cctggcatat attttgcctc aatcgcaaaa tcaataatgc gatcgatgcg ccatttacca
     9901 cacatttatt atggttatcg atatcatgat actgcggcgg ggcgctaaaa cgaagggaag
     9961 aagcattagt gtaattaatt aaagcaagat aatacgtatg tttgatcaat tttcgaggcg
    10021 ataaccggcc ataaaaaagc gcaacgtaga acaggaatta ttctcacgaa agaaatccat
    10081 taccatctct ttatccagtc catagcggcg cgtcagaata cccgcttccc aggcgctaag
    10141 ctggcgatct ccggtctttt cgtacatccg gtgtgagtag cctaatacaa aaccgcgttt
    10201 ataatcggca cagaattttg tcacattaac cgcactgtcg gcatgcgttg cttttaatcc
    10261 cgccattaag cctttaccaa aatggttatt catcgttcac ctcattcgct atatattatc
    10321 atatatagca atattttagg caaagaacga cttttgcgca cgctattttt tcatgcttgt
    10381 ttaagacgta atcccgtaag agataaaccc atcaggagag atatttatcg aaccatttca
    10441 gcgtcctttg ccaggcaaga tcggcggcag atttgtcata acgaggcgtg gaatcattat
    10501 ggaatccgtg attaaccccc ggatagatat acgcctcata gaccttatta ttggctttca
    10561 acgccgcctc ataagcgggc cagccctcgt tgattcgggt gtccagttcc gcgaagtgga
    10621 gtagtaaagg cgcttcaatc ttcgccacat cggcagtggg tgcctgacga ccataaaacg
    10681 gcaccgcgca ggccagtt
//
LOCUS       DAJVUJ010000145        10660 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.145, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000145 DAJVUJ010000000
VERSION     DAJVUJ010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10660
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.145"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            311..1609
                     /gene="kgtP"
                     /locus_tag="OQQ48_003334"
     CDS             311..1609
                     /gene="kgtP"
                     /locus_tag="OQQ48_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HCS1953990.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1606..1929)
                     /locus_tag="OQQ48_003335"
     CDS             complement(1606..1929)
                     /locus_tag="OQQ48_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HCS1953991.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1975..3330)
                     /gene="pssA"
                     /locus_tag="OQQ48_003336"
     CDS             complement(1975..3330)
                     /gene="pssA"
                     /locus_tag="OQQ48_003336"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HCS1953992.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYKAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENSGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRLFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3444..6104)
                     /gene="pat"
                     /locus_tag="OQQ48_003337"
     CDS             complement(3444..6104)
                     /gene="pat"
                     /locus_tag="OQQ48_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HCS1953993.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQRKMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPSALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AISNPLDLRDDASSEHYIKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RIHGLLVQSMANRAGAQELRVVVGHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            complement(6136..6834)
                     /gene="tapT"
                     /locus_tag="OQQ48_003338"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6136..6834)
                     /gene="tapT"
                     /locus_tag="OQQ48_003338"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HCS1953994.1"
                     /translation="MTENTVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREALAEGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6903..7322)
                     /gene="trxC"
                     /locus_tag="OQQ48_003339"
     CDS             complement(6903..7322)
                     /gene="trxC"
                     /locus_tag="OQQ48_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HCS1953995.1"
                     /translation="MNTVCTHCQSINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7529..8566
                     /locus_tag="OQQ48_003340"
     CDS             7529..8566
                     /locus_tag="OQQ48_003340"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCS1953996.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAELVQSITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8614..9303)
                     /gene="ung"
                     /locus_tag="OQQ48_003341"
     CDS             complement(8614..9303)
                     /gene="ung"
                     /locus_tag="OQQ48_003341"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCS1953997.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            9608..9991
                     /gene="grcA"
                     /locus_tag="OQQ48_003342"
     CDS             9608..9991
                     /gene="grcA"
                     /locus_tag="OQQ48_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HCS1953998.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10047..10634)
                     /gene="eamB"
                     /locus_tag="OQQ48_003343"
     CDS             complement(10047..10634)
                     /gene="eamB"
                     /locus_tag="OQQ48_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HCS1953999.1"
                     /translation="MTPTLLSAFWTYTLITAMTPRPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
BASE COUNT         2654 a         2704 c         2739 g         2563 t
ORIGIN      
        1 aaaggccatc cgtcaggatg gcctttttgc attggcgcag aaaaaatgac tgatgcgacg
       61 ctgcgcgtct tatactccca catatgccag attcagcaac ggatgcggtt tccccaactt
      121 gcctacttcc atacgtgtcc tccttaccag aatttatcct taagctcctc aataaccatt
      181 ttcctgctaa ctaaattcat ggttaatgtt gcataataat acgtaacaaa tgcataacat
      241 tttctttacc aaaagaaata aacaaaagtg accaacaaaa gcatcggatt acggcaggag
      301 acataatggc atggctgaaa gtactgtaac ggcagacagc aaactgacaa gtagtgatac
      361 tcgtcgccgc atttgggcga ttgtgggggc ctcttcaggt aatctggtcg agtggttcga
      421 tttctatgtc tactcgttct gttcactcta cttcgcccac attttcttcc cttccgggaa
      481 cacgacgact caactactac aaacagcagg tgtttttgct gcgggattcc tgatgcgccc
      541 aataggcggt tggctatttg gccgcatagc cgataaacat ggtcgcaaaa aatcgatgct
      601 gttatcggtg tgtatgatgt gtttcggatc gctggttatc gcctgcctcc caggttatga
      661 aactataggt acgtgggctc cggcattatt gcttctcgcg cgtttatttc agggattatc
      721 cgttggcgga gaatatggca ccagcgccac ctatatgagt gaagttgccg ttgaagggcg
      781 caaaggtttt tacgcatcat ttcagtatgt gacgttgatc ggcggacaac tgctagccct
      841 actggttgtc gtggttttac aacacaccat ggaagacgct gcactcagag agtggggatg
      901 gcgtattcct ttcgcgttag gagctgtgtt agctgttgtg gcgttgtggt tacgtcgtca
      961 attagatgaa acttcgcaac aagaaacgcg cgctttaaaa gaagctggat ctctgaaagg
     1021 attatggcgc aatcgccgtg cattcatcat ggttctcggt tttaccgctg cgggctccct
     1081 ttgtttctat accttcacca cttatatgca gaagtatctg gtaaatactg cgggaatgca
     1141 tgccaacgtg gcgagtggca ttatgactgc cgcattgttt gtattcatgc ttattcaacc
     1201 actcattggc gcgctgtcgg ataagattgg tcgccgtacc tcaatgttat gtttcggttc
     1261 gctggcagcc atttttaccg ttcctattct ctcagcattg caaaacgttt cctcgcctta
     1321 tgccgctttt ggtctggtga tgtgtgccct gctgatagtg agtttttata catcaatcag
     1381 tggaatactg aaggctgaga tgttcccggc acaggttcgc gcattaggcg ttggtctgtc
     1441 atatgcggtc gctaatgcta tatttggtgg ttcggcggag tacgtagcgt tgtcgctgaa
     1501 atcaatagga atggaaacag ccttcttctg gtatgtgacc ttgatggccg tggtggcgtt
     1561 tctggtttct ttgatgctac atcgcaaagg gaaggggatg cgtctttagt gacgggtcag
     1621 ttgccagacg gtatagccgg tgcttgcacc ggcgacatcc caggccaaat ccttccagct
     1681 ccagccgctc ccttcggggc ggctatccca aagctctttt gacgccccca aactgacaga
     1741 gaacatcaat ccaaacatgg cactgcgatc ccggctcatc ccctgatgct gtgaatattc
     1801 atttccggcg gcggaaagca tcgccgaggc gataaagtgt tgagctttat cctgcccgct
     1861 ccagctatcg ttagccatat ggctgcatcc agaaagtaac aatagcgaac agacaaaaag
     1921 aatacgcaaa acgtggcctc caaaaaacaa accccgtaca ggacggggtt gtgattacag
     1981 gatgcggcta attaatcggt cgatgcggat acggcgcaaa cggcggatga gtttacgaac
     2041 cttcaccgga taatcggcaa tactttgcag atcgcgatag tgcttaacga tggtggtatg
     2101 ctcgcggatc agctccagtt ctttctctcg ctgcggcgca agctccagtt gtggatcgtg
     2161 gatcaaaatg gcgttttcca gatccagacg ccaggcgcgc gggttcaggt tattaccggt
     2221 gatcaacatc cacttatcat caacccacat ccctttcagg tgataggtgt tgtcgtcatc
     2281 tttccataac cgaaccacta gctggtcagt attgacgtaa tactgcaaac ggctcaggaa
     2341 aagacgcaga ttgatctcat agagataagg caatgcgcca attatcttga aaggttcatc
     2401 ttccggaatg tagaagtcgt tcgcggtttt atcaccaaca ataatttcga cctttttccc
     2461 ttcgcgcagc aactggataa tattgcgcac aaggattgct ggcaggttga agtatggcgt
     2521 acagatggtt agtttctgct cggcacaagg cataagatgg aaaatggtct tgttcaacag
     2581 actcgatttc cccagcccca ctagcggcgt tacggaaagc tgatcgttgt cggcatcgcc
     2641 ctggaaatga taagcggcat cacgcagctc ctggcggaac agacgaatat cgttcttgat
     2701 ttccgggctt tttggccgat taacatcatc cagacgatta acgccgcggc cattcataat
     2761 attctgtgta acccattcaa acataatgtc tgacatctta cggttacgga taagatgata
     2821 acggtcgtag cgatatttat cgtgctgatg caggtaaaca tcgttcaggc tggcaccgct
     2881 ataaagtacg ctatcgtcga tgataaagcc tttaaagtgc agaacaccaa gggcttcacg
     2941 ggtattgatt ggaacgccat aaaccggaac atctacgccc gaattttcct gcgccatgcg
     3001 gcagtaccag tcagcgttag tgttagatgc cgcagcgcca atgcgtccac gttgtgcacg
     3061 atgccagtcg accagcaccc gcacatccag ttccggacgc tgccttttag ctttatacaa
     3121 cgcgttcaga atgcctttgc caccgtcatc ctgttcgaga tacagggcga caatgcaaat
     3181 gcgctgcttc gcgctggcta ttttttccag cagcgtctcc cggaagtcgg cgggagcgta
     3241 aaagaaatcg acatcatcaa ctgattgaga aatcttgggt agttgggcaa ggtgttgttg
     3301 atgtttatta cgcttaaatt ttgacaacat cacagtgcat ttcttctctg ttcattgaag
     3361 gatcctctgt gcaatgcaga cgacataagc gggcaataat aacaccagtc ccgattaagt
     3421 agtcaacatt tccagtacct tactcatgat tcctcgcgtt gggcaagatt tagcgtaagc
     3481 ccaacgatcc cctcttcgag ctggatatca acgttaaacc cgagcttgcg ggctagcgcc
     3541 accatgccac gattgtttgg catcgtaata ccattcagac gttgtagtcc gtgatctcgc
     3601 gtataggtaa tcaacttttc cattaagcgt cgacctaagc ctaacccttt gagatccgag
     3661 cgaaccagca cagcaaattc ggcatcgatg ttatcaggat cggaaatcgc acgcgtgacg
     3721 ccgaggatct cttccgtttg atcaatacgt cgtaccgcta caaacgccat ttcccgatcg
     3781 tagtcgatct gtgtcatgtt ggctaaatct tcatgggtaa attcgttgat ctcgctaaag
     3841 tagcggtaat aaagatcttc tttggtgact cgcgaaatga attgctgaag ttgtggctca
     3901 tcttctggca aaatcgggcg gaacaagcag cgttcaccgt ttttcaattc tacccattct
     3961 tccagctgat gcggataagg gcgcactgcc agccgactct cgttatcgcc ttcaaacggc
     4021 gagatatcca gcgtgacatc cagcgcggta aattcactgc cagaagccag caaaggatga
     4081 atatccagac gctgaatttc cgggcaatcg acaatcaagt tggaaacctg caccagaagc
     4141 tggctcaagc ctgcaacatc caatgggcgt agcgcactgc gcgcacgaat ctttttactt
     4201 ttgatcccct gaataaccag atagcgggcc aggttcatgt tcagcggcgg cagtgcgacg
     4261 acggcttgat cttcaggacg ccactccaca ccgccttcac ccagcatgat caacggcccg
     4321 aaaaccggat cgtgcccaac cacaacccgc aactcctgag cgccagcacg gttagccata
     4381 ctttgcacca acaggccgtg gatccgcgcc tgcggccagg ccatttttac gcgatcgaaa
     4441 atggcgtttg ccgcttgctg gacttcattg gctgtacgca agtacagcat gacgccctga
     4501 acttccgatt tatgtggaat atccggcgaa cgcaatttca gcgccaccgg ataaccaatc
     4561 tgttcggcaa tatgcaccgc ttcggtgcta tcgctggcaa tccaggtagg gagcgtgttc
     4621 atgccatacg cttgcaggat gggctgaact tcatgggtat cgagcgacgt agccccttcg
     4681 gcaatcgcct gttgcaacag aagatgcgct tctgcggtat tggaagtcag attgctgggc
     4741 aacgccggcg tttcgcgtag ttgcttctga ttacgccggt actccaccat atgcataaaa
     4801 gcagtgatgg ttccttccgg ggtacggtag gtcggcagcc cggcttcgct gaataaacgt
     4861 cgtgcctctt gcgaggagtg ctcgccgcac cagttcgtca gcaaagagac atatttgctg
     4921 cggggatgat gctttaccgc ttcaattaat acttgcgcgc tttctgttgc gggagcagcg
     4981 gcgctgggcg aatgaataac catcagcgcg tcaaaatcct ggctgtggag cagaatatcc
     5041 agcgttttaa tatagtgctc actgctggcg tcatcgcgta gatcgagcgg gttagatatt
     5101 gccacatgtt ctggcagtgc atcgcgcagt ttctggcagg tttcttcgct tagcgttgcc
     5161 agcttgccat tgcgtgacca taaggcatcc agcgccagcg cggaaggcgc agcaccgttg
     5221 ctgataatca tcagccggtc gccacgtagc gggcgcatat ggctaagggt ttccaccgcc
     5281 gaaaacagct cgtgggtgtc ctgtacccgc aacaaaccgg cacgctgaat agccgcatcc
     5341 catgccgggt ccattcctgc cgtggtgttg agcagtcgct gtgccgccgg gctacgtccg
     5401 cttttaatca ccagaatcgg tttattacgc gaggcactac gggccgccga aacaaagcgt
     5461 cgcgcgtcgc ttaactgttc gagatagagc aggatggcgc tggttttact gtcgcgtgcc
     5521 agatagtcaa gcaattcatc aacgtcgata tccaggctgt cgccgagcgc aataaagtag
     5581 gaaaagccca tcttacgctg ttgcgcccag tcgaggatgg tgttggagac ggcagccgat
     5641 tgcgaaataa acgccagctt gccgcgttta atcggcacag gcgaaaagct ggcattcaga
     5701 ccttgccagg gagccagtaa acccagactg tttggtccaa gcaggcgcat gttatggcgc
     5761 agggcgcagg cgcggagatc ttcgtgttgc gatgccgggg cggaaagaat aatgcaggtt
     5821 ttacagcctt tctcgccgag ctcttccaga agagcaagat tacggctggc attggtacat
     5881 aaaaccgcaa ggtcgggtgt aaagggcaag ctggcaatat ccggccaggc caacacaccc
     5941 aacaccgctt tccaggctgg cgtcaccggg agtaccggtc cgttaaagcc tcccgccagc
     6001 aggttacgca tcatcaggta acctgcgcga ttgggtttca tcgacgcgcc aattaccgct
     6061 atcgattttg gtcgcagtag tgcttccagt cctcgctgac tcataccggt ctcccgcaga
     6121 agtgaccgaa tgattttaaa cgctttctaa ctgttctgct gtgatgctac ccagatgttg
     6181 cgttttccct gccagatagc gtgttttaaa gcgggtaaaa tgctcgccta accctgctgc
     6241 cgccccggta tcgccggcca tatctaacag tgcgatggct acctcggcag tacaatattg
     6301 gccttcagcc agggcttcac gcaggcgata ggcagaaagc cgggaaagat cgacggaaat
     6361 gacgggaaga ttatccagat acggactttt acgaaacatc ttgcgagctt ccggccaggt
     6421 accatcgagc atgataaaca gcggtggctt accggcaggt ggtgtgaaga tcacttcccg
     6481 ttgctcatca gcatacgagg cgggaaagac caccattggc tgataatccg ggttttgtac
     6541 cagatccagc aaatcctgcg agggttcggt acgcgaccat tgaaacgcaa cggtatcagg
     6601 caaaatatca gcaatcagac gcccggtatt actgggcttc attggctcgg tgtcgaacat
     6661 cagcaaacag aagcgacttt ttgcttgtgc tggggtaatt gtcgaacaga gacataattt
     6721 ctctggcaaa agacagcgtt ggcagcgacg aacgcgatta ccgcgggcaa gaaaaggacg
     6781 tgttgcgcgc gcaatacgct cggcgcgtaa ctggagaaca gtgttttcgg tcataagaga
     6841 gcgtcgaaaa aacgccattg tcgcagagga gaaaacgggg cacaagatgc gccccggtaa
     6901 gattaaagag attcgttcag ccagctatcg aacggagctt tcggtactgc gccattaagc
     6961 atgtcgacaa cctgaccgtt tttgaaaatc atgatcgtcg gtatactacg aattccaaag
     7021 cgactgctca attcacgttc agcttcggta ttcactttca caaagcgcac tttaccgcta
     7081 cgctcttgcg cgacatcttc aaaaattggt gcgaaattac ggcaggggcc gcaccacggt
     7141 gcccagaagt cgatcaccac aggtagatca tccttcagca atttgtcgag tgtttcaccg
     7201 gtcgcattaa tcacctctcc gtcaaacaag tcgtgaccgc agcgtccgca ttttgccgca
     7261 tcttcgatcc gatcgtcggg aatgcgattg atggactgac aatgggtaca aacggtattc
     7321 ataactaacc tcgggatgag taaggagact tatcggcaac gacgccggta tgtttctaat
     7381 atgttacata ttatcgggga cattgttcca gacaacaatc tgttttattc aataggtatg
     7441 atagtcgcag gctttggtac gaaatcatgg agaaccgcgc ggacttcggg taatctgcgc
     7501 gcttcgcgca gcgctggtgg agaaaagcat gaacgatgaa atgaaaggta aaagcggcaa
     7561 ggtcaaagtg atgtatgtcc gcagtgatga tgattctgat aaacgtaccc acaacccgcg
     7621 taccgggaaa gggggcgggc gtccaggaaa atctcgtgct gacggtggcc gtcgccccgc
     7681 ccgcgatgac aaacagagtc agccccgtga ccgcaagtgg gaagattcgc cgtggcgcac
     7741 ggtttcccgc gcgccgggtg atgagacgcc ggaaaaggcc gatcacggtg gcatcagtgg
     7801 taaaagtttt attgatccgg aagtgttgcg tcgtcagcgt gcggaagaaa cccgcgtcta
     7861 cggcgaaaac gcctgtcagg cactgttcca gagccgtccg gaagcaattg ttcgcgcctg
     7921 gtttatccag agcgtaacgc cacgttttaa agaagccttg cgctggatgg cggcaaaccg
     7981 caaagcgtac catgtggtgg atgaagctga attgacaaaa gcgtcaggca cggaacatca
     8041 cggcggcgtt tgcttcctga tcaaaaagcg taacggtaca accgtgcagc agtgggtaag
     8101 ccaggcaggc gcgcaggatt gcgttctggc actggaaaac gaatctaacc cgcataacct
     8161 gggtggcatg atgcgcagct gcgcgcactt cggcgtgaaa ggtgtcgtgg tgcaggatgc
     8221 ggcactgctg gagtcggggg cggctatccg taccgcagaa ggcggcgcag agctcgttca
     8281 gtcgattact ggcgacaaca ttgttaacgt gctggatgat ttccgtcagg caggttacac
     8341 cgtagtgacc acttccagcg agcagggtaa accgctgttc aaaactagtc tgccagcgaa
     8401 aatggtactg gtgctgggtc aggaatatga agggttaccg gatgccgcac gcgatccgaa
     8461 cgatctgcgc gtgaagattg acggtacggg caacgttgcc gggctgaata tctctgtcgc
     8521 aaccggcgtt ttgcttggtg aatggtggcg tcagaataaa gcctgataaa tcagccgggt
     8581 ggcaactctg ccatccggca tttccccgca aatttactca ctctctgccg gtaatactgg
     8641 catccagtca atcggcgtct cgccacgttg ttccagccac tgatttgcca gcacaaaatg
     8701 gttgcagcca aagaatccac gatgcgccga aagcggcgac ggatgcggtg ctttcagtac
     8761 atgatggcgt tgcttatcta taatcgcccc tttcttttgc gcatgtgatc cccacaacaa
     8821 aaacaccacg ccttcgcggt gctggttaat caggctgatg actttatcag taaacgtctc
     8881 ccaaccgagg ctggcgtggg aatgcgcctg acctgcgcga accgtcaaca cagtattgag
     8941 cagcagaacg ccctgacgcg cccagctttc gagatagcca tgattagggc gggtgaagcc
     9001 cggaatagta ttttccagct ctttatacat attcaataat gacggaggaa tggcaatgcc
     9061 gggacgaacg gaaaatgcca gaccatgcgc ctgtcccggt ccgtgataag gatcctggcc
     9121 gagaatcacc actttaacgt cacccaactc ggtaaagcgg aacgcgttaa agacatcttt
     9181 ttgtggtgga tagatagtga cgccggactg ccgctcgctg gcgacggtct gaagggtatt
     9241 aagaaaataa ggttgttgct tctcttcagc cagcacgtca tgccaggtta attcgttagc
     9301 catctcgctc tcctgcgaat cttcaatccg cctagcttaa ctgcttcttt ctacagaaca
     9361 aaatcatgct cattttttag acttaaacga gtgcaaaaaa atgtaaattg gtaaaatttc
     9421 acacccgcat aagttgatgt aaaacaataa aatccattta cgaccacaat cgatgtgtgg
     9481 tttttattga tttaaatcaa agaatcaagg gtggttgagg agtatatata cactcaagca
     9541 acaatggttt taccaattag ccgcgacagg ctgaacaaat caaataattt tgccggggag
     9601 gcatcacatg attacaggta tccagattac taaagccgct aacgacgatc tgctgaactc
     9661 tttctggctg ctggacagcg aaaaaggcga agcacgttgc atcgttgcaa aagcaggtta
     9721 tgcagaagat gaagtggttg cagtaagcaa actgggtgac attgaatacc gtgaagttcc
     9781 agtagaagtg aaaccagaag ttcgcgttga aggtggtcaa cacctgaacg ttaacgttct
     9841 gcgtcgtgaa actctggaag atgcagttaa gcatccggaa aaatatccgc agctgaccat
     9901 ccgtgtatcc ggttatgcag ttcgctttaa ctctctgact ccggaacagc agcgcgacgt
     9961 tatcgctcgt acctttactg aaagcctgta aggcttaagt agcaagttac taagcggaag
    10021 agggcgacct cttccgcttt ttttcgttaa tagaaaatgc gtaccgcgca atagaccagc
    10081 aacagggcaa gcacgatatt taactggcga ccatactggc gaaacaatcg ctgaaacaga
    10141 tgccccgcca gcgcccagca cacattgcca aacgtcccaa tcatcgccag caaaacgctg
    10201 acgccaacta cccagcttag cgcctgtgtt tgcggcagaa caaacgtcga cagtgccgta
    10261 acaccgtaca aaatgatttt gacgttcaca aactgcaaag caaagctggc ccaaaagctg
    10321 attggttttg cctgaagtcc gtcttccttt gttgggctgg tggcgatttt ccacgccagc
    10381 cagacaatat atgccgcccc cgcccaactc aaaaggtgta ccgctgccgg gtcaatcact
    10441 gccagtgaaa atgaaatgcc cgcacacagt aacatcacaa tcaaaaatcc cagactcatc
    10501 cctgccagca cgcgggtact ttgacgaaat ccatgcgacg tagcagagct aagggcgaga
    10561 atattgttcg gtcttggcgt catagcggta atcagggtgt aagtccaaaa agcacttaaa
    10621 agggtcggtg tcacttgtta tctcctctgt tttggtagac
//
LOCUS       DAJVUJ010000146        10607 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.146, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000146 DAJVUJ010000000
VERSION     DAJVUJ010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10607
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.146"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..637
                     /gene="arnT"
                     /locus_tag="OQQ48_003344"
     CDS             <1..637
                     /gene="arnT"
                     /locus_tag="OQQ48_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="catalyzes the addition of
                     4-amino-4-deoxy-L-arabinose to lipid A; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose lipid A transferase"
                     /protein_id="HCS1954000.1"
                     /translation="LALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVF
                     CAWSIFSLWAFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEM
                     TQESLQPSRYILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDE
                     FANWLNQHRQEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            634..969
                     /gene="arnE"
                     /locus_tag="OQQ48_003345"
     CDS             634..969
                     /gene="arnE"
                     /locus_tag="OQQ48_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HCS1954001.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            969..1355
                     /gene="arnF"
                     /locus_tag="OQQ48_003346"
     CDS             969..1355
                     /gene="arnF"
                     /locus_tag="OQQ48_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HCS1954002.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(1349..1615)
                     /gene="pmrD"
                     /locus_tag="OQQ48_003347"
     CDS             complement(1349..1615)
                     /gene="pmrD"
                     /locus_tag="OQQ48_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HCS1954003.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(1725..3080)
                     /gene="menE"
                     /locus_tag="OQQ48_003348"
     CDS             complement(1725..3080)
                     /gene="menE"
                     /locus_tag="OQQ48_003348"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HCS1954004.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQY
                     ALVLEGENTFPALTSLHIQMVEGTHSALWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLLFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(3077..4039)
                     /gene="menC"
                     /locus_tag="OQQ48_003349"
     CDS             complement(3077..4039)
                     /gene="menC"
                     /locus_tag="OQQ48_003349"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HCS1954005.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELLQMPSIAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALRLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSTLPVVEVDALERLL"
     gene            complement(4039..4896)
                     /gene="menB"
                     /locus_tag="OQQ48_003350"
     CDS             complement(4039..4896)
                     /gene="menB"
                     /locus_tag="OQQ48_003350"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HCS1954006.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNVGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(4911..5669)
                     /gene="menH"
                     /locus_tag="OQQ48_003351"
     CDS             complement(4911..5669)
                     /gene="menH"
                     /locus_tag="OQQ48_003351"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HCS1954007.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(5666..7336)
                     /gene="menD"
                     /locus_tag="OQQ48_003352"
     CDS             complement(5666..7336)
                     /gene="menD"
                     /locus_tag="OQQ48_003352"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HCS1954008.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPSLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(7425..8720)
                     /gene="menF"
                     /locus_tag="OQQ48_003353"
     CDS             complement(7425..8720)
                     /gene="menF"
                     /locus_tag="OQQ48_003353"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HCS1954009.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLEEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(8799..9104)
                     /gene="elaB"
                     /locus_tag="OQQ48_003354"
     CDS             complement(8799..9104)
                     /gene="elaB"
                     /locus_tag="OQQ48_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HCS1954010.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQVSDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(9159..9620)
                     /locus_tag="OQQ48_003355"
     CDS             complement(9159..9620)
                     /locus_tag="OQQ48_003355"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCS1954011.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLDT
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            9685..10602
                     /gene="rbn"
                     /locus_tag="OQQ48_003356"
     CDS             9685..10602
                     /gene="rbn"
                     /locus_tag="OQQ48_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HCS1954012.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
BASE COUNT         2426 a         2993 c         2702 g         2486 t
ORIGIN      
        1 tctggcgctg cggattaatg gctggattaa catcgctttt ggcgtcactg gcattattgc
       61 cacatttgtg gtctccccgt ggggaccaat gaacacgccg gtgtggcaaa ccttcgagag
      121 ctataaagtc ttttgtgcct ggtcgatttt ttcgctatgg gcatttttcg gctggtacac
      181 cttaacaaac gtcgaaaaga cctggtcttt cgccgcgctt tgcccgctgg ggctggcgtt
      241 gctggtagga ttttcaattc ctgacagagt catggaagga aaacatccgc aattttttgt
      301 cgagatgaca caagaatcac ttcaaccaag ccgctatatt cttactgata gcgtcggtgt
      361 tgccgcaggt ctggcatgga gcctgcaacg cgatgacatc atcatgtatc gccagacagg
      421 tgagttgaaa tacggcctta attatccgga tgcgaaaggg agatttgtca gcggtgatga
      481 gttcgcaaac tggcttaatc aacatcgtca ggaggggatt attacactcg tgctttcggt
      541 tgaccgcgat gaagatatca acagtctcgc cattccgccc gcagatgcca tcgatcgtca
      601 ggagcgtctg gtgctgattc agtatcgtcc caaatgatct ggctaacatt agtctttgcc
      661 agcttgctta gcgttgccgg gcagttgtgt cagaaacagg caacctgctt tgtggcgata
      721 aacaaacggc gcaaacatat cgtgctgtgg ctgggactgg cgctggcttg tcttggtctt
      781 gccatggtgc tctggctgct ggtcttgcag aacgtaccgg taggcattgc ctacccgatg
      841 ttaagtctga attttgtctg ggtgacgctg gctgcagtaa aactgtggca cgaaccggta
      901 tcgccgcgtc actggtgtgg ggtggcgttc attattggtg gcattgtgat cctcgggagt
      961 acggtgtaat gggcctgata tggggattat tcagcgtcat tattgcctca gttgcgcaat
     1021 taagcctggg ttttgcggca agtcatctgc cgccgatgac gcacttatgg gattttatcg
     1081 cggctctgtt agcttttggc ctggatgccc ggatactgtt actcgggctg ctgggttatc
     1141 tgctttccgt tttttgttgg tataaaacgc tacataaact tgcgctaagc aaagcctatg
     1201 ccttgctcag tatgagttat gtgctggtat ggattgcttc aatggtactt cccggctggg
     1261 aagggacttt ttcgctgaaa gcactactgg gagtagcctg tattatgagc gggttgatgc
     1321 tgattttcct gcccacgaca aaacaacgtt actgagtttt ccctgccgct gtacactgcc
     1381 gttcccactc atcggggcta taactgcttg ccgagaggac tttcaccgta tgcagctttt
     1441 cacgattaat acaataaagc gcattctgca aaggcgagag taaatccccc actttcacgg
     1501 cgaagtcact cttcacttcg gcgatcattt ttatcgcgcc gccagcatcg cagagcataa
     1561 gtacatgtct gttatcttgt ttattgcagc acgatttttt gaccagccat tccattgcat
     1621 tatcctgttt gctaagagtt ttcacatcaa tttacagatt aacagataaa ctctttatca
     1681 acggcaatgg aatggcagaa atgagaagtg aaacggaggg ttaattattg ctgacgttgc
     1741 acccactctt ttagcgcctg acgtgaaatt ttaataccgc cgtttttcag ctccggcggt
     1801 aaagtcagcc agcgcaccgg ttgttgaaaa cgagccagct tatctttcac ccattcgcca
     1861 agatcaacgg tttgctggtc atactccacc accgctaccg gccgatgacc aaactccttg
     1921 tcggcaacgg gaacgataaa cacctgcaaa accgcaggat gtgcagcaat aacacgctcg
     1981 acttcttccg gctgaatacc ctcaccgcca ctgaataaca gattgtctaa acgtccgaca
     2041 atggtcagct tgccattatg catctcaccg cgatcgcgcg tagcgtacca gccttcgtca
     2101 ttaaccagtg aaaccagttg cccgttacgc cagtaacctt ctgccatact ggcagcccgc
     2161 agccacactt cattattaac gattttcact tcccgacccg gcagcggcga accaacgtct
     2221 gccaggccgt cggcttcttt cgcacatacc gtggaggcaa actcggtcag accatagccg
     2281 caaaagcaac gaatcccctg ctcgcgcgcc tgttccgtca actcgaccgg gatagccgcg
     2341 ccgccaagta acaccgcttt cagggaaacg gaactgcggt taaccagcaa acgccagagt
     2401 tgtgttggca ccagtgaagc gtgagtacag cctgccagca tttgctccaa tggctgttta
     2461 tcacgcaccg tcatccgcgc accagcgtat aaccagcgcc acataattcc ctgaccggag
     2521 acgtgaaaca gcggtaaaga gagcaaccag tcatcgtgat cgccaaacgg aatcagcgat
     2581 aacacacctt gcgcactggc aagatgggcc tgataagtat ggacagcggc tttcggtaaa
     2641 ccggtggagc cggaggtcaa tgtcattgaa caaagacgtg ttggttgcca cagagctgaa
     2701 tgtgtgcctt caaccatctg aatatgcagt gaagttaacg ccgggaaagt attttctcct
     2761 tccagcacca gcgcatattg cagtgtaaga tttggcagta attcctcaag caacggttgc
     2821 ggaagctgtg gatttacggg cagcacccgc gccccgcatt gcagtaacgc cagccaggcg
     2881 agcagcgttt gcggcgtatt ccacgcccgc aacatcacgc cgctgccctc aaccacaccc
     2941 tgcgccgcaa agcccgtagc taactcatcg acgcgagcac aaagctcgcg ccagttgagt
     3001 tgctcgtcat taagacgtaa ggcgatggct tctccccgca cttgccgcca gtgacgccac
     3061 ggccagtcag agaagatcat aacaaccgct ccagtgcatc aacttccacg acaggcagcg
     3121 tgctacccgg ccagcgacgt acctgctgcg cctgcatcag atccagcgtg tccagccctg
     3181 gaatggtgtc cggcgttaac caggcggcaa tccgcgccag ttgcgttaag cctaagctcg
     3241 actcaataga agaactgatc accgccgtca gcctcaacgc gtgcgccgcc tgtacctgct
     3301 cgcgtacttt ttccagactg cccgtgagcg tgggtttgat aactaccgcg cgcacgccct
     3361 cttcagccac aaaggcaaaa tccggctcgc gcaggctttc atcccaggca atggcaatgc
     3421 cggtttcacg ggcaaacgct cgcgaatcat cgcgggtttt gcacggctct tcgagaaacg
     3481 cgatgcggtg gcgataatcc gggttaacat atttggcaaa ctgctgacct ttcagcggtg
     3541 tccaggcgcg atttgcgtca agacgcaaat gcagatccgg aattgcctcc agcaacagat
     3601 taaccaccat gccgtcgcgc acggcttcgt acaatcccac tttgaccttc gccactttct
     3661 cgcctggcat atcagcaagt ttgaggatca gatcgtcagg atcgccatta cacagtggtg
     3721 ccgcacggta attggccgcc tgcgggagcg tatctgccag ctccgccaat gcacagctta
     3781 cgccaaaggc aatggaaggc atctgcagta gctcgcaatc gcctgccagc cagttattta
     3841 cccaggcaag cagcacgctt tgcgcctctt cccaggtttc ctggctgaag cccggcagtg
     3901 gggagatctc cccccaccct tcgcgctcgc cctcacgcag gcaaacgtac agcccgtcgc
     3961 gggtttttaa ccgcctgtcg cgcagaacca cccccgcgtc catggggatc tgccagcggt
     4021 atacctgcgc gctacgcatt acggattccg tttaaatttg ctgaaatcag gctgacgttt
     4081 ctggttgaaa gcgttgcgac cttcctgacc ttcttccgtc atgtagaaca gcatggtggc
     4141 gttgcccgcc agctcctgca gccccgcctg cccgtcacag tcggcgttca gtgcagcttt
     4201 caggcagcgc agcgccatcg gactgttttg cagcatttcg cggcaccagc gaacggtctc
     4261 tttttccaga tccgccagcg gtaccacggt gttgacaagg cccatatcca gcgcctgttt
     4321 cgcgtcgtac tgacggcaca ggaaccagat ttcccgcgct tttttctgtc cgacgatgcg
     4381 agccatgtag gaagcgcccc agccgccgtc gaaggaaccg actttcgggc cagtctgacc
     4441 gaagatggca ttatctgccg cgatagtcag gtcgcacatc atgtgcagaa cgtgaccgcc
     4501 gccgatggag tagccagcca ccatcgcgac aaccggtttc ggacaggtac ggatctgacg
     4561 ctggaagtcc agcacgttca ggtgatgtac gccggaatca tctttatagc cgccgtaatc
     4621 accacgcact ttctggtcac cgccggagca gaacgcttta tcacctgcgc cagtcagaat
     4681 gatcacgccg acgttgtcgt cataacgcgc atctgccagt gcctggatca tctcttttac
     4741 cgtcagagga cggaaggcat tgcgcacctg cggacgatta atggtgattt ttgcgatacc
     4801 atcggtggat ttttcataac gaatgtcctc gaaaccttcg gagcagtcgt gccattcaac
     4861 cggtgcgtaa agcattgctt catcaggata aatcatattg tgtcctttag tcagaaacgc
     4921 aagatctgcg ccagacttgc gattacgcca gcgggatttt cccgatgcgc gttatgtccg
     4981 gcgcgaggaa tgacatggca gtcggcagcc agttccgccg ccagggcgcg gaatttgctg
     5041 tcacgttcac cacataaata ataaaacgca aatgtgcggg cgctaaggtt agcacgtaaa
     5101 tcaggctgga cggcgagaga agtcgcctcc agcatggcgg caagggttgc gccattattg
     5161 ttgctgcgca gcgccaccag ctcccggcgt tgatcgtcat tgagcgaggc aaaaacaggc
     5221 tgttgatacc agtcggcaaa taccgccgtt aacggttctg tgcgaaaacg ctgcgcccat
     5281 tggcgatcgg aacgctgacg ttccgcacgt tgttcagcat tttgcagccc cggatgcccg
     5341 ccttcgacaa taaccccaca aagccccgcc agcccctggc aagccgccat catcgccacc
     5401 cgtccgccaa gcgagtaccc caccagccag aagtcaagga tgttgtaact aaccaaggtt
     5461 ttacgcagta agtcggtgac atcatcaaat ccatcgacgc taatcgccgc cgaaccacca
     5521 tgacctggga gatcaacata caaccgtgag tagtcggcaa acgcctcgcc cacttcttgc
     5581 cattcgtggc aatcgccgga aaaaccgtgc aaaaacacca gccagggtaa acctggtttt
     5641 ccgtgttttg cctgcgcgtg caggatcata aatggcttac ctgcgccaga agttgctgga
     5701 gcgtttgcgc accatcggtg tcgttaacca ccatttcaat caccgtggtg gttggcgtgc
     5761 gccaggcgtc ggcaaatgcc gtttcaagtt cctgccagtt ttgcggacga tgatatttca
     5821 gctcgaacat cgcggcggcg tgctcaaaat ggacgttttg cggcatcaga tagaaacgct
     5881 cacgctcgct ttgcggcgtt ggcaacagcg agaaaatttg cccgccgttg ttgttcacca
     5941 caattaatac cagcggcgca gaaacctgac gcaataacgc cagcgcgttg agatcgtaaa
     6001 gtgcggagag atcgcccaca atcgccagcg tcggtttgcc gcttgcccgc tgaacgcctg
     6061 cggcggtcga aagcagtccg tcgataccgc tggcccctcg gttgctgtac accgggtaac
     6121 ctgccggaag ttgcgaaagc gcatcaatca gacgtaccac caggctgtta ccgacaaaca
     6181 attgcccctg ttcaggcaga tagtcgctga tgcgatgcgc cagttgcgct tcgccaaacg
     6241 catcgcgacg ggcaataacc gcctgcattg cctgttccgc caggcgcggg atttcaacgc
     6301 accagggctg gcgtttttct gccggatgca gctccagcca gtcggcaata ttggcaatta
     6361 agcggcgtcc acgatggtgc gccggatcaa gccgcccttc gatgtcatcc acaatccagt
     6421 actcttcggg ttcacagctt gcctgccatt gcaggagccg tttgcccgtc aggctgcttc
     6481 ccagttgcac cacaatttgc gcctgctgca gctcgctggt cgctttggca ttgcctaacc
     6541 aaagatcggc acacggcagc ggctgcccgg tttgtgacag cacatcgcca atcagcggcc
     6601 agccaagagt ttgcgcccac agggcaactt ttttgccctc ttccgcactc atgcgcccgg
     6661 caaccaccac gccgcgcttt tgtcgccaga agaaccagtc gcgctgtttt tcactttcca
     6721 gactaggcgc ttcacgcagc cacggtttgt cgtcctgcca ccagtcaccc agacgctgtt
     6781 gccagctaag cccggtatcg tccatttcgc catacagcgg ttcagcaaac gggcagttga
     6841 tatggactcc ccccgcatga agcgtaccga gagcgtggtc gatggtagaa accagccaac
     6901 gtgcggggat atcctgggtc gggcgcggca atgaaatact gtgcgtgggg tgagaggcga
     6961 acattcccgg ctggcgaatt gcctgattcg cgccgcagtc aattagctcc ggcgggcgat
     7021 cggcggttaa gagaatcagt ttttctccgg ttaacccggc ttcaatcagt gccggataga
     7081 gatttgccac cgcagtgccg gaggtcacaa tcaccgccac cggctgcttg ctgactttcg
     7141 ccagccccag cgccagatgc cccaacccac gctcatcgaa atgggtgtgg tgaatgaatg
     7201 cggaattctc cgccgccgct aacgttaacg gtgtagaacg cgagcctggg gcgatacaga
     7261 tgtgtctgac gccgtgacgc gttaatgctt ccagaatgac cgccgcccag cgtcggttaa
     7321 atgcgcttac tgacatgagt ttgtccggta tcaatattgc ggctaagtat aaggagctac
     7381 aaaaatagat tattgatatg aatcggtaat gatgcgactc attactattc catttgtaat
     7441 aaagtacgca accctgccgc tttgttgtcg atttcctgcc actcttgctc ggggtcggaa
     7501 ccacggacaa tgcccgcgcc agcatataat cgcacgacat tgccgctaat ttttgctgag
     7561 cgcagggaaa cgcagaattc gctttgttgt aatgagagat agcccgcaga accggcgtac
     7621 cattctcggg tgaacggttc gtgacgggcg ataaactgtc gcgccagatc gcgcggtaag
     7681 ccagcaactg ctgccgttgg ctgcaactga tgtaaacaga tcacatcatc cgctttgttg
     7741 agtgaagtcc agatacagcg gcgaagatgc tgcactttac gcagacgcag tacctgcggc
     7801 ggtaaaacat ccagcgtctg ggtatcggcc tgtaatcgtt ggcagatatc ttccaccacc
     7861 agcatgttct cgcgctggtt tttatcatcc gccatcagcc actcttctaa ctgctgcgcc
     7921 tgcttatcat caggatgatt tgctactgtt cccgccagcg cttcagtacg cagcgctttg
     7981 tcacgccgcc gccataaccg ttccggtgaa gagccaagaa aagcattttc gccatcaaag
     8041 gccatgtaaa aatggtagca attcagattc agtcgacgac tggcagccat catcgccgcc
     8101 gcgttgaccg gacttgcgaa atgcaggtca gttgcccgag cgagcaccac tttgtcgagc
     8161 tcaccttcgg cgatggtttt cgttgccagt tcgattaatt gcgtccagcc cgttttgtcc
     8221 ggccagtgtt gttctcgcgt ggtggttaaa tgtaacccag gcaagggctt gatactcacc
     8281 agtgtggcga taaattcttt tgcctgaatc gcatcgtgct gaagggagct ttcgctgaat
     8341 agcgtcagcc gcagcgtggc tttaccgcca cagcgtcgcc attccaggcg gggtaaaagt
     8401 aaattgccct gcgacgggtc aaaagcattc agcccccaaa tgcgtaagtc ggcgtgttcc
     8461 gggtgctggc gaagaaaacg ttgtgcctgg tccaacgacg taaaacgggt aatcgcgccc
     8521 aggacggcag cttcttcatc accattacgt tgttgccagt agaattgcgg gtacgtttgc
     8581 tgactggcca gccagctcaa ggcatcaaaa gcgtctttga gagggaaagg aatatcgata
     8641 acccgaatgc cgggtgtcgc cggaatctct tgcgacaaat ggcgcagtag attttccagc
     8701 gccgtagtaa gtgattgcac gcggacctct ccctgttaaa agcctcacat tatacggggt
     8761 actacaaaaa aaatgcagta ccccggtgta gggaggtttt aacggcgtgc cagcaacagt
     8821 cctagtacca gcccaacggc cgcgcccaca ccaattcctt gccaaggttt ttcgtggacg
     8881 tagtcatcag cacgataaac agcctgcttc gcccgatagt aataactgtc tgaaacctgg
     8941 ctaacccgtt ttttcacatc atccagcgct ttttctgcac gcgctttcag ctcaacatat
     9001 ttctgatcag cgggatcgcc agaggagcgg agcacctctt ccagtgtctc actaagcagc
     9061 gtcaggtcgt catcgatacg tgtatcacca aactgattag acattctcgt tctccatttg
     9121 cgtaaaacct gtcactaact atagacaagg gttgttgatt acgcctgaat tacctcgcgt
     9181 gccatcccaa tgtgtgggat accatcttct tcatagacct ccgtcacagg gataaaaccg
     9241 aaactctggt agaagttttg cagatgcgcc tgcgccccga ggtaaacagg cttatcaggc
     9301 cagtgatgcg tgcaggtgtc gagggtttta ctcatcagtt gctgccccac tttttcgcca
     9361 cgcaacgctt cactgacaat cacccgacca ataacgaccg gctcaagatc atcatcactt
     9421 ttcagaatcc tcgcatatgc caccagttca tcatttttcc aaccgagaat atggcgatta
     9481 tcccccgtca aatcatcacc atcgatatcc tgataagggc aattctgctc gacaacaaac
     9541 accgcgcaac gcagttgtaa taaggcataa agctgggaaa ccgataattc agagtgatgc
     9601 agatcttgcc attcaatcat aatgctctcc ttttagatgt ataccttata ctagccggaa
     9661 tattttttga aacgtgatga actcatggaa ttaatttttt taggtacttc agccggtgtg
     9721 ccaacccgca cgcgcaatgt cacggcaata ttgcttaacc tgcaacatcc gacccagagc
     9781 ggactttggt tgtttgactg cggtgaaggc acccagcatc agctactgca taccgccttt
     9841 aaccctggaa aactggacaa gatttttatc agtcaccttc atggcgatca tctttttggt
     9901 ttacccggct tgctgtgcag tcgttctatg tcaggcatta tccaaccctt aacgatttat
     9961 ggtccgcaag gtatccgtga atttgtggaa accgcgctgc ggattagcgg ctcatggacc
    10021 gattatccgc tggaaattgt cgaaattggc gctggcgaaa ttctcgatga tggcctgcgc
    10081 aaagtaaccg cttatccgct ggaacaccca ctggaatgtt atggctatcg tattgaagaa
    10141 catgataaac cgggtgcatt aaatgcccag gcattaaaag ctgctggcgt gccgcctggc
    10201 ccactgtttc aggaattaaa agcgggcaaa acaatcacgc tggaagatgg aaggcagatt
    10261 aacggcgcag attacttagc tgctccagtg ccaggtaaag cgctcgctat tttcggcgat
    10321 accggcccct gcgatgccgc acttgacctg gctaaaggtg tcgatgtcat ggtgcacgaa
    10381 gcgacgctgg atataaccat ggaagccaaa gccaatagtc gcggccatag ctctacacgc
    10441 caggctgcga cactagcccg tgaggctgga gtcggtaagc taatcattac ccacgtcagc
    10501 tcgcgctatg atgacaaagg ttgtcagcac ctgttacgtg aatgcaggtc aattttcccg
    10561 gcgactgaac tagcgaatga tttcaccgtg tttaacgttt aactctg
//
LOCUS       DAJVUJ010000147        10534 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.147, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000147 DAJVUJ010000000
VERSION     DAJVUJ010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10534
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.147"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(88..237)
                     /gene="hokB"
                     /locus_tag="OQQ48_003357"
     CDS             complement(88..237)
                     /gene="hokB"
                     /locus_tag="OQQ48_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HCS1954013.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            636..2249
                     /gene="trg"
                     /locus_tag="OQQ48_003358"
                     /pseudo
     CDS             636..2249
                     /gene="trg"
                     /locus_tag="OQQ48_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
     gene            complement(2287..3209)
                     /locus_tag="OQQ48_003359"
                     /pseudo
     CDS             complement(2287..3209)
                     /locus_tag="OQQ48_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            3426..4769
                     /locus_tag="OQQ48_003360"
     CDS             3426..4769
                     /locus_tag="OQQ48_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCS1954014.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GGPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEYRLIADVVCFP
                     RCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHLGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            4994..6649
                     /gene="opgD"
                     /locus_tag="OQQ48_003361"
                     /gene_synonym="mdoD"
     CDS             4994..6649
                     /gene="opgD"
                     /locus_tag="OQQ48_003361"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HCS1954015.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            6789..7013
                     /locus_tag="OQQ48_003362"
     CDS             6789..7013
                     /locus_tag="OQQ48_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HCS1954016.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGFDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKGV"
     gene            7076..7612
                     /gene="rimL"
                     /locus_tag="OQQ48_003363"
     CDS             7076..7612
                     /gene="rimL"
                     /locus_tag="OQQ48_003363"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HCS1954017.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(7607..8587)
                     /locus_tag="OQQ48_003364"
                     /pseudo
     CDS             complement(7607..8587)
                     /locus_tag="OQQ48_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8711..9703
                     /gene="tehA"
                     /locus_tag="OQQ48_003365"
     CDS             8711..9703
                     /gene="tehA"
                     /locus_tag="OQQ48_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HCS1954018.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATMPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSENGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            9700..10293
                     /gene="tehB"
                     /locus_tag="OQQ48_003366"
     CDS             9700..10293
                     /gene="tehB"
                     /locus_tag="OQQ48_003366"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HCS1954019.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDRQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKLGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
BASE COUNT         2693 a         2461 c         2680 g         2700 t
ORIGIN      
        1 aacatgcact aactgaagtg taaaaggggt gccatgagaa aatgaatctg ctagtcagat
       61 gcgcggggaa aatccccgcg cttgccctta cctggacgtg caggccatga gcgcagcaac
      121 ctccttatca ccgtcccgga accgcagttc gtagagcgtt tgtcgggtca ggagtgtgaa
      181 tgtcagaatc gtaatgcaga taatgagcag acacaccacc agagggttgt gcttcatagc
      241 ctttctcctt gccggatggc gggtaagagg ctaagatctg aattgctagg ttcattcgtt
      301 ggcctcggtt gatagaaata tcggtcgggg ccttcgtctt tctgattccc ggttagcctg
      361 aaaacagaaa gtctcaggca cccgcaggca tcctatgagg tttccttagg gacgaaaata
      421 atcacttcac gaaattgcgt gctgttttcc agaatttttc gtcattcgga ttagccagtt
      481 tagccattcg ttactctctt cattccaata gcattaattt tctctgcaat aattgttgta
      541 aaaatgtgac gcaaagaggt ttttggtcat aagtaattac cgtcaagtgc cgatgacttt
      601 ctatcaggag taaacctgga cgagagacaa cggtaatgaa tacaactccc tcacagcgat
      661 taggtttttt gcatcacatc aggctggttc cgttatttgc ctgcattctt ggcggtatct
      721 tagttctatt cgcattaagt tctgctctgg caggccgatc gcgatcagcg tgatgttact
      781 gcggagattg agattcggac cgggttagcg aacagttcag attttttgcg ttcagcccgg
      841 atcaatatga ttcaggccgg ggctgcgagt cgtattgcgg aaatggaagc aatgaagcga
      901 aatattgcgc aagccgaatc ggagattaaa cagtcgcagc aaggttatcg tgcttatcag
      961 aatcgaccgg tgaaaacacc tgctgatgaa gccctcgaca ctgaattaaa tcaacgcttt
     1021 caggcttata tcacgggtat gcaacctatg ttgaaatatg ccaaaaatgg catgtttgaa
     1081 gcgattatca atcatgaaag tgagcagatc cgaccgctgg ataatgctta taccgatatt
     1141 ttgaacaaag ccgttaagat acgtagcacc agagccaacc aactggcgga actggcccat
     1201 cagcgcaccc gcctgggtgg gatgttcatg attggcgcgt ttgtgcgtgc cctggtcatg
     1261 acgctgataa catttatggt gctacgtcgg atcgtcattc gtccactgca acatgccgca
     1321 caacggattg aaaaaatcgc cagtggcgat ctgacgatga atgatgaatc ggcgggtcgt
     1381 aatgaaatcg gtcgcttaag tcgtcattta cagcaaatgc agcattcact ggggatgaca
     1441 gtagggactg ttcgacaggg cgcggaagag atttatcgtg gcaccagcga aatttcagct
     1501 ggcaatgcgg acctgtcatc tcgcaccgaa gaacaagcgg cggctatcga acaaactgcc
     1561 gccagcatgg agcaactcac tgcgacggtg aaacagaatg cggataacgc gcatcatgcc
     1621 agcaaactgg cgcaagaggc ttctattaaa gccagcgatg gcgggcagac ggagtaaaaa
     1681 cgatgggcgc tatctccacg agttcgaaga aaatttctga gatcaccgcc gtcatcaaca
     1741 gtattgcttt ccagacgaat attctggcac tgaatgctgc cgttgaagcc gcgcgagcgg
     1801 gtgagcaagg gcgtggattt gccgttgtcg ccagcgaagt acggacactc gcaagtcgca
     1861 gcgctcaggc ggcgaaagag attgaaggct tgatcagtga atcagtcagg ttaattgacc
     1921 tggggtcgga tgaggtggca acggccggga aaaccatgag cactattgtt gatgccgtcg
     1981 cgagtgtcac acatatcatg caggaaatcg ccgccgcctc ggatgaacaa agtagaggca
     2041 taacgcaggt tagccaggcg atttctgaaa tggataaggt gacgcaacag aatgcttctc
     2101 tggtagaaga ggcctcagcg gcggcggtgt cccttgaaga acaggcggca cgattaactg
     2161 aggcggtgga tgtattccgt ctgaacaaac attctgtgtc ggcagaacct cgcggagcgg
     2221 gtgaaccagt tagtttcgct acggtgtgaa aatgttcaag gagggatcga cagatccctt
     2281 cacctttcag aacggcattg attttcgaat agcgttaatc atcaactggc aaccagaaga
     2341 gaacatcgca tctacgcggg tcagtattcc aatcggttcg cctgcaccat gtcccggaac
     2401 aggctgggcc accagcgtgg catgacgcag gtcgtctttt accgcgccag aagggacaaa
     2461 ccacacgtag tcgtattcaa ccgtaagttg acgagatagc gaagcagaca gcgtttcgat
     2521 acaacccgaa ggaattttac atccctggct ctgtactaat gcatctgaat gctggcgtgg
     2581 cgcagtgcct tctggtgata caacgaccgg ccatttcagc actcggctta gcgttacgtt
     2641 ctcctgaagt agcgggtgat tagggcggac aaccagcttc agcgattcaa gaaacagcag
     2701 ttcgtaatta agcccggtca tcagttcagg atctgacatc cgaccaatgc cgatatcgat
     2761 ttccccggtt ttcaaacccg ccagaatcat agggttactc attgtcgcaa cttgcaaggt
     2821 cgtctctttt tgttgctgat gaaactgacc tataaccgaa ggtaatatcc ccagtgccgc
     2881 agtaggtagt gcaccaatcc tgacgacatc attattaaga ccttctttac gatgaagcga
     2941 ctgtccggca gtgttgatgg cgtcaagaac tctgactgca tgcgttaaaa attgttcgcc
     3001 gggtaaggta agttgcgccc cctgacgacc acgctcaaac aagcgagcgc ccgtcagctg
     3061 ctacagttca ttcaatgtct tagagagcgc aggttgactc aaattaaggg tttcagccgc
     3121 gcccccaaag ttccttgttg tgcgacagct acgaatgtat gaaggtggcg caaacggatg
     3181 cgctgactaa acagactatt tttttccata agcgatgtta aaaacgaagc ggtgtcgctg
     3241 acaagtgaag ttgtttgatt atgataactt gattgcaaaa tataattaac aattaaagca
     3301 attgtgttac agcaaaatgg ataatattga tgttttcgcg gcgagatcac agtttgtaaa
     3361 ttcttcccgc cagagtgaat gcggttacct acactccaga ttactgacca ctggaggcag
     3421 acactatggc gaacagcatc acggcggatg agattcggga acagttttcg caggcaatgt
     3481 cagccatgta ccagcaagaa gttccgcagt acggcacgct gctggaactg gtagctgatg
     3541 tgaatctggc tgtgctggaa aacaatcctc aactgcacga aaaaatggta aatgcagatg
     3601 agctggcgcg actgaatgtt gaacgtcatg gggcgattcg cgttgggact gcacaagagc
     3661 ttgctactct tcggcggatg tttgccatta tggggatgta cccggtgagc tattacgatc
     3721 tctcgcaggc aggggggccg gtacattcga cagcatttcg gcccattgat gatgcttctc
     3781 tggcgcgtaa tcccttccgc gtttttacct ccttactccg ccttgagctt atcgagaacg
     3841 aaattttgcg ccagaaagcg gcggagattc tgcgtcagcg cgatatcttc accccacgtt
     3901 gtcgacaact gttagaggaa tatgagcagc agggcggttt taacgaaaca caggcacagg
     3961 aatttgtgca agaggccctg gaaacgtttc gctggcacca gtcagcaacg gtagatgaag
     4021 aaacctatcg cgccttgcac aacgaatatc ggttgattgc tgatgtggtc tgttttccta
     4081 gatgccatat caaccacctg acgccacgta cgctggatat tgaccgggtg cagtcgatga
     4141 tgcctgaatg cggaattgaa cccaaaattc tcatcgaggg gccgccgcgc cgcgaggtac
     4201 cgattttact acgccagacc agctttaaag cactggaaga gacggtgttg tttgcggggc
     4261 agaaacaggg cacgcatacc gcgcgctttg gtgaaattga gcagcgtggc gtggcattaa
     4321 cgccgaaagg gcgacaactg tatgatgatc ttctgcgtaa cgctggaacc gggcaggata
     4381 atctcactca ccaaatgcat ttacaggaaa ccttccgcac ttttcctgac agtgagtttt
     4441 taatgcgtca gcaagggttg gcatggttcc ggtaccgtct gacgccttcg ggtgaggcgc
     4501 atcgtcaggc gattcatctt ggagatgatc cacagccttt aattgaacgt ggttgggtag
     4561 tggcgcaacc catcacctat gaagatttct tgcccgttag cgcggcgggg atcttccagt
     4621 caaatctggg taatgaaacg cagacacgca gtcacggtaa tgccagtcgc gaagcatttg
     4681 agcaggcgtt gggttgtccg gttttggatg agttccagct ttaccaggaa gcggaagaac
     4741 gcagtaaacg tcgctgtggt ttgctttaaa ttctgaccat ccgcctttgc aaaaatttgc
     4801 ctgattttac aaacgaatca ggctcatccc atcgacataa aaaaaatgcc gatttatgca
     4861 tattctctca gttcaacaat tggattatta ataaatattg tctagagtga gcggtcataa
     4921 ataagcactt tcttgccgct gaaaacgacc agcgcgggac cattcacaac accagaagga
     4981 ctcactttca ggtatggatc gtagacgatt tattaaaggt tcaatggcta tggccgccgt
     5041 gtgcggtacc agcggcattg cttctctttt ttctcaggcg gcattcgcgg cagattctga
     5101 tattgccgac gggcaaaccc agcgttttga cttctccatt ctacagtcaa tggcgcacga
     5161 cttagcgcaa acagcgtggc gtggtgcgcc tcgtccgtta cctgacacgc tggcgacaat
     5221 gacgccgcag gcttataaca gtattcaata cgacgccgaa aaatcgctct ggcataacgt
     5281 tgagaaccgt caactggacg ctcagttctt ccatatggga atgggattcc gtcgccgcgt
     5341 tcgtatgttt tctgtagatc cagcaacaca tctggcgcgt gaaattcact ttcgcccgga
     5401 gttgttcaaa tacaacgatg cgggtgttga tacaaaacaa ttagaagggc aaagcgatct
     5461 cggctttgcc ggttttcgcg tgtttaaagc ccccgaactg gcgcgccgtg atgtagtatc
     5521 atttctcggc gcgagttatt tccgcgccgt tgatgataca tatcaatacg gtttgtcggc
     5581 ccgcggcctg gcgatcgaca cttacaccga cagtaaagaa gagttccccg actttaccgc
     5641 cttctggttt gatacggtaa aaccgggggc aactaccttt accgtttatg cgttgctcga
     5701 tagcgccagc attactggtg cctataagtt cactatccat tgtgagaaaa gtcaggtgat
     5761 tatggatgtg gaaaatcacc tgtatgcgcg caaagacatt aaacagctgg gcattgcgcc
     5821 gatgaccagt atgttcagct gcggtactaa tgaacgtcgg atgtgcgata caattcatcc
     5881 gcaaattcat gactctgatc gtctgtccat gtggcggggc aacggcgagt ggatttgccg
     5941 tccgctgaat aatccgcaaa aattgcagtt caatgcttac accgacaaca acccgaaagg
     6001 gtttggttta ttgcaactgg atcgtgactt ctcccattat caggacatta tgggctggta
     6061 taacaaacgc ccaagtctgt gggtggaacc gcgtaacaag tggggtaagg gcaccatcgg
     6121 cctgatggaa atcccaacaa cgggcgaaac gctggataac attgtctgtt tctggcagcc
     6181 agaaaaagct gtaaaagcag gtgatgagtt tgcattccag tatcgtctgt actggagtgc
     6241 gcaaccgcct gttcattgcc cattagcgcg cgttatggcg acgcgtaccg gcatgggcgg
     6301 tttcccggaa ggttgggcgc caggtgaaca ctatcccgaa aaatgggcgc gtcgttttgc
     6361 cgtcgatttc gttggtggtg atctgaaagc tgccgcgcca aaaggcattg agccggtgat
     6421 tacgctttcc agtggggaag cgaagcaaat cgaaattctc tatattgaac ccatcgatgg
     6481 ttatcgtatt cagtttgact ggtatccgac ttcggactcc actgatccgg tcgatatgcg
     6541 gatgtatcta cgttgtcagg gggacgctat cagtgaaaca tggctgtatc agtatttccc
     6601 gccagcgccg gataaacgtc agtatgttga cgaccgcgtg atgagttaat cgttttttct
     6661 tcggcacctt cttcgggagg tgccgtctgg ttaaacacga tcccgctcgc atttttccct
     6721 aagttaaatg agtaatctga tggtgtgtat ttcagataca ccttgtcagc cactaacagg
     6781 gagtgcgtat gtttccagaa taccgagatt taatatcccg tctgaaaaac gaaaatcctc
     6841 gctttatgtc cttgttcgat aaacacaata aacttgatca tgaaattgcc agaaaggaag
     6901 gtttcgacgg tcgagggtac aatgcggaag tggtccgcat gaaaaaacaa aagctacagt
     6961 taaaagatga gatgctcaaa atcctgcagc aggagagcgt caaaggggtg taaactttcc
     7021 taagccgcca gccaggcggc ttttttaaca acagcatgga ttgactggag ataagatgac
     7081 tgaaacgata aaagtaagcg aatcacttga attacatgct gttgcggaaa gtcacgtcac
     7141 acctctttat cagttaatct gtaaaaataa aacctggtta cagcagtcgc taaactggcc
     7201 gcagttcgtt cagagtgaag aggacacgcg aaaaacggtg cagggtaatg tgatgttgca
     7261 tcaacgcggc tatgccaaaa tgttcatgat tttcaaagaa gatgaactta tcggcgttat
     7321 ctcgtttaat cgtattgaac cactgaataa aactgctgaa atcggctact ggctggacga
     7381 atctcatcag gggcagggga tcatttctca ggcgctgcag gcattgattc atcattacgc
     7441 tcagtctggt gaacttagac gcttcgtgat caaatgtcgg gtggacaatc cgcaaagcaa
     7501 ccaggtcgct ttgcgcaatg gttttatcct tgaaggttgc ctgaaacagg ctgagttcct
     7561 gaatgatgcc tatgatgatg tgaacttata cgcgcgtatt atcgattcat aataaccctg
     7621 ccagcggcgt tcgcgtaatg cgctcctcgc cgttaatgac ttttggacca cggacatgaa
     7681 ccgtgtcgcc atcaaaggct tcaacgacgg cgtggtcggt taactccaca tggttttcaa
     7741 ttatcaccgc acctgaaata cggctctcac cttgtattac aacgtgctcg tcgagcagaa
     7801 tcggttcacc acgtaccacg gcatttccgc caatcaagac atgatgtttt aacacacaat
     7861 taccttccac aatggcatat tccgccacct gcgaactgta atgaatcgtc ggaatggcat
     7921 cttcttctat gccagctttc acctgcgcgt gaccgtagac tttagcgcaa tcgcatagcc
     7981 agacgttgtt ctcttcatta ccttcaacac tggcaaaatc aaaaacttcg gcgcgatgtt
     8041 caataaaagc ataccggacg acggcatcgc cataaatttg tgcctggtgg acaatacgcg
     8101 aggcgctaac ccttgcgcga tcataaattt ggagcaggag ttgatggtcg ggcgttaagc
     8161 cttgtgcggc gacgatcata gaatgttgat caattaaggc gtgaccaaat attctacatt
     8221 gcccgtaaac taatgagtca tgaatggtga cactgtcact tatatatgca ccctgactga
     8281 tttcactatt atcgatccag acgttatccg ttgcgtaaac ctctccccac agcacgctgg
     8341 tgccggtgat acgcgtatta ccagaaatca ccgttccgcc gaacgcaata gcattctggt
     8401 cgtaaatcca gcaatttcct tcctatgcta acaccgtttc gcgatcgatc cagccgccag
     8461 cggttcctgc tatcacatcg ttaaaatcgc taatggcgat aatctgccgt aataacacac
     8521 tttttttagt gccatcttcc tgataactaa aggctcgctg ttcttcactt agacgatatt
     8581 tgcgcatagc gttttctcac aggtgactta ctatgaccgt agcaaattct gcggctctgg
     8641 ctatgctcga gaaaattcca taaaatgcat ttcaaatata ctttataaat taaacaaaat
     8701 gagtaagaag atgcagagcg ataaagtgct caatttgccg gcaggctact ttggtattgt
     8761 gttggggacg atagggatgg gatttgcctg gcgctatgcc agccaggttt ggcaggtcag
     8821 ccactggtta ggggatgggc tggtgattct ggcgatgatc atctggggat tattaactag
     8881 cgcatttatt acccgactca tacgctttcc gcatagcgtg ctggcggaag ttcgccatcc
     8941 agtgctgagc agttttgtga gtttgtttcc ggcaacgacg atgctggtgg cgattggttt
     9001 tgttccgtgg tttcgcccac tggcggtgtg cctgttcagt tttggtgtcg tggttcagtt
     9061 ggcttatgcc gcctggcaaa ctgcgggatt atggcgcgga tctcaccctg aagaagctac
     9121 catgcctgga ctgtatctgc cgacagttgc caacaacttt atcagcgcaa tggcctgtgg
     9181 tgcgttgggc tacaccgacg ccggtctggt gtttttaggc gcaggcgttt tctcatggct
     9241 aagccttgaa ccggtgatct tgcagcgtct gcgcagttcg ggagaattac ccacggcact
     9301 gcggacatca ctcggcattc agctcgctcc tgcgctggtg gcctgtagtg cctggttgag
     9361 cgtcaacggc ggcgagggtg atacgctggc gaaaatgctt tttggttatg gactgctgca
     9421 actgctgttt atgctacgtc tgatgccatg gtatctctcc cagccattta atgcttcatt
     9481 ctggagtttc tcgttcggcg tatctgcact ggcaaccacc ggtttgcatc tggggagtgg
     9541 cagcgaaaat ggatttttcc atacgctggc ggtaccgctg tttatcttta ccaattttat
     9601 tattgcaata ctgctcatcc gtacttttgc gcttctgatg cagggaaaat tgttagtcag
     9661 aaccgagcgc gctgttttaa tgaaagcaga ggacaaagaa tgatcattcg tgacgaaaac
     9721 tattttactg ataaatatga attaacccgc acacactctg aagtactgga agcggtgaaa
     9781 gtggttaaac cgggtaaaac gctggatctg ggctgtggca atggtcgtaa cagtctttac
     9841 ctggcagcca atggttatga tgttgacgca tgggataaaa atgccatgag tatcgccaat
     9901 gtagagcgca ttaaatccat tgaaaatctg gataatttac acacccgagt cgttgatctg
     9961 aataacctca catttgatag acagtacgat tttattcttt cgactgtggt actgatgttc
    10021 cttgaggcta aaaccatccc agggctgatt gccaatatgc aacgttgcac taaacttggt
    10081 ggttacaacc tgattgtggc ggcgatggat accgctgatt atccatgtac cgtcggcttc
    10141 ccgtttgcct tcaaagaggg agaattacgt cgatattacg aaggctggga gagggtgaaa
    10201 tacaatgaag acgtcggcga gctgcaccgc accgacgcca acggtaatcg tattaaactg
    10261 cgtttcgcca cgatgctggc acgtaaaaaa tgacccgcta agaataaggc gcgtgaaaat
    10321 tccccacgct gagatgattt actgttcttc ttttcggtaa gcatattttt tatcgaaggg
    10381 atgtgaaatt aatcacagta gtcgaagttt tttagcagct taacttactg aaatttaact
    10441 actgatgatt gacttagccc ctttttcggc attgactatg tcgtctgaaa aggggctgaa
    10501 aaatttattt tcaccaacac tttttttgcc acaa
//
LOCUS       DAJVUJ010000148        10373 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.148, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000148 DAJVUJ010000000
VERSION     DAJVUJ010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10373
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.148"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(40..1035)
                     /gene="gapA"
                     /locus_tag="OQQ48_003367"
     CDS             complement(40..1035)
                     /gene="gapA"
                     /locus_tag="OQQ48_003367"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCS1954020.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            1377..1790
                     /gene="msrB"
                     /locus_tag="OQQ48_003368"
     CDS             1377..1790
                     /gene="msrB"
                     /locus_tag="OQQ48_003368"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCS1954021.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            1832..2104
                     /locus_tag="OQQ48_003369"
     CDS             1832..2104
                     /locus_tag="OQQ48_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HCS1954022.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(2265..2906)
                     /gene="pncA"
                     /locus_tag="OQQ48_003370"
     CDS             complement(2265..2906)
                     /gene="pncA"
                     /locus_tag="OQQ48_003370"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HCS1954023.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(2917..3933)
                     /gene="ansA"
                     /locus_tag="OQQ48_003371"
     CDS             complement(2917..3933)
                     /gene="ansA"
                     /locus_tag="OQQ48_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCS1954024.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPDDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(4100..5956)
                     /gene="sppA"
                     /locus_tag="OQQ48_003372"
     CDS             complement(4100..5956)
                     /gene="sppA"
                     /locus_tag="OQQ48_003372"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HCS1954025.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDHNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPSAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            6117..6668
                     /locus_tag="OQQ48_003373"
     CDS             6117..6668
                     /locus_tag="OQQ48_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HCS1954026.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            6785..7828
                     /gene="selD"
                     /locus_tag="OQQ48_003374"
     CDS             6785..7828
                     /gene="selD"
                     /locus_tag="OQQ48_003374"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HCS1954027.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            7833..9794
                     /gene="topB"
                     /locus_tag="OQQ48_003375"
     CDS             7833..9794
                     /gene="topB"
                     /locus_tag="OQQ48_003375"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCS1954028.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEVGSGAIA"
     gene            9921..>10373
                     /locus_tag="OQQ48_003376"
     CDS             9921..>10373
                     /locus_tag="OQQ48_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1954029.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWEIFNSNFERMIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLITPYRFYHKQWSETLEFWPRKPEPGKDTFQYH"
BASE COUNT         2612 a         2535 c         2736 g         2490 t
ORIGIN      
        1 accgaagtcg ctctttttag atcacagttt catctcaact tatttggaga tgtgagcgat
       61 caggtccaga actttgttgg agtaaccggt ttcgttgtcg taccaggata ccagtttcac
      121 gaagttgtcg ttcagagcga taccagcttt agcatcgaac acggaagtgc aaacttcgcc
      181 gttgaaatcg gtagatacta cgtcatcttc ggtgtagccc agaacgcctt tcatttcgcc
      241 ttcagcagcg gctttaacgg cagctttgat ctgctcgtaa gttgcagctt tttccagacg
      301 aacggtcagg tcaactacgg atacgttcgg ggtcggaacg cggaacgcca taccagtcag
      361 tttgccattc agttctggca gtactttacc tacagcttta gcagcaccgg tagaggacgg
      421 gatgatgttc tgggaagcgc cgcggccgcc gcgccagtct ttgtgagacg ggccatcaac
      481 ggttttctga gtagcggtag tagcgtgaac ggtggtcatc agaccttcga tgatgccgaa
      541 gttatcgttg ataactttag ccagcggagc caggcagttg gtggtgcagg aagcgttgga
      601 aacgatgtcc tggccagcat atttgtcgaa gttagcgcct ttaacgaaca tcggagtgtt
      661 gtctttagac ggaccagtca taaccacttt cttcgcacca gcggtgatgt gtttacgagc
      721 agtttcgtca gtcaggaaca gaccagttgc ttcagcgaca acgtcaacac caacttcgtc
      781 ccatttcagg ttagccggat cacgttcagc ggtaacacgg atttttttac cgttaacgat
      841 cagatgaccg tctttcactt caacggtacc gtcgaaacgg ccgtgagtgg agtcatattt
      901 cagcatgtat gccatgtaat cagcgtctaa caggtcgttg attgcaacga tctcgatgtc
      961 agaacgtttc tgagcagcac ggaaaacaat gcgaccgata cggccaaaac cgttgatacc
     1021 tactttgata gtcatatatt ccaccagcta tttgttagtg aataaaaggt tgcctgtaaa
     1081 attacaaaaa ccttacgcag cgtcaagcgg aatcgtgtca atcattgcga caaatcaatc
     1141 ctgtgcctaa gcattatgcg actgactcgc ctcactcttc ctttgggctt gaaaccacat
     1201 ggggtcggcg ccccgaattt taaagggcaa ttacgataaa aatgtgattt tcatcacgat
     1261 ttaggtgcag ccatttcgtc gagatcaagt ttaggacaaa attttgccag ttggtgttaa
     1321 tgttttgtta gaatcagtca cgtaatgtga gcacgattaa agtgagatgt gagcaaatgg
     1381 ctaataaacc ttcggcagaa gaactgaaaa aaaatttgtc cgagatgcag ttttacgtga
     1441 cgcagaatca tgggacagag ccgccattta cggggcgttt actgcataac aagcgtgacg
     1501 gcgtatatca ctgtttgatc tgcgatgccc cgctgtttca ttccgaaacc aagtatgatt
     1561 ccggctgtgg ctggcccagt ttctacgaac cggtgagtga agaatccatt cgttatatca
     1621 aagacttgtc acatggaatg cagcgcatag aaattcgttg cggtaactgt gatgcccatc
     1681 tggggcatgt ctttcccgac gggccgcaac caacaggcga acgttattgt gttaactctg
     1741 cttctttacg cttcactgat ggcgaaaacg gcgaagaaat caacggttga aaaaacgatt
     1801 cagcaactta ttccacagga gcggaaaaat tatgaatctt gatgacatta tcaacagcat
     1861 gacgcctgag gtataccagc gtttgtcgac cgccgttgaa ctggggaaat ggcctgatgg
     1921 cgttgcgttg accgaggaac aaaaagagaa ttgcctgcaa ctggtgatgc tgtggcaagc
     1981 ccgccataat accgaagcac agcatatgac aattgacaca aatggtcaga tggtcatgaa
     2041 gagcaaacag cagttaaaag aagattttgg tatctcagca aagcctattg ctatgtttaa
     2101 gtgaatgagt tatctagtat ttaagcctgt tattaaggga gggcaaatat acttgttcgg
     2161 acgtcgtgtt ttagctgcaa gtaggttaaa tgcaacactg acaagtatta tctggcctac
     2221 aatgtcattg aggaaatgta ggccagagat gaattcaact aatattaccc ctgtgtctct
     2281 tcccagtctg ccagcgtata tagcgttgcc ccagctgctg acatctccat aaacgcgtgt
     2341 gcactgtcct ggggctggat attcacgcca cgacaaccat cggtaatcac gttcacctta
     2401 taacctaact gtaacgcgtc cagcacggta aacttcacgc aatagtcagt agccaggccc
     2461 ataacgatca attcatcgat ttcatgatcg cgtaaccagt catcgagaga ggttttctgc
     2521 cgacggccgt tatcaaaaaa ggcactgtaa ctgtcaacta aaggattttc gcctttatgg
     2581 aacaccgctg cgatcgcttt ttggttcagt aacggatgta attgtgcgcc ttcactgttc
     2641 tgcacacagt gatctggcca gaaggtttgt ggcaaaccgt cgagttggcc tggcgtataa
     2701 ggctctacac cgtgctgact ggcaaaactg ccgtgattcg ccggatgcca gtcctggctg
     2761 gcgataaccg cttctccgcg cgactggcac cagtcaataa ggcggttagc aacctccacc
     2821 gtactgtcac cttccggtac ggcgagcgcg ccaccagcac agaaatcatt ttgtaaatcg
     2881 accagtaaca gggcgcgatg gggcattgca gtctccttaa tcatccggcg tcagttcgcc
     2941 gcgcaggttt tggctcatgg ccttgcgaat ggtttcagta tccagttcct ggctcagcag
     3001 gtaatgcagt ttggttagcg tggcttctac agtcatatct gcaccgccaa taacgccggc
     3061 atgggcgagg gcgttaccgg tggcataacc acccatattc actttaccgg acatacattg
     3121 tgtcaggttg accaccacaa taccgcgatc gctggcttct tgtaattcct gcaggaaggc
     3181 tttgttttgt ggcgcattac ccacgccata ggagcgcaga atcaatgctt tcactggttg
     3241 gcgcagaaaa ttgcgcacga catcagcaga aatccccgga taaatcgtca ctacgccaat
     3301 tggttgtggc gtgattggat gaacgattaa ttccccttca ccgtgcgggg cgggtggcgt
     3361 attcaaacga cgaatatgga taccagcttc cagtaacgga ggaaggtttg gagaggcaaa
     3421 cgcatcaaaa ccatcggcat gggctttggt agtgcggttg ccgcgataca atcggttatt
     3481 gaaaaagagc gttacttcgt tgatcggata attcgccgca acgtacaacg cattcagcag
     3541 attaatttgt ccgtcagagc gtaactcagc cagcgggatt tgtgaccctg tcacaatgac
     3601 cggtttaccg agattctcga gcatgaacga cagcgcagag gcggtatacg ccatcgtgtc
     3661 ggtaccgtgt agaatgacaa aaccatcata gtcgtcatag tgcgctttaa tatcttcagc
     3721 aatatgctgc cagtcatccg gcgtcatatc agaagaatcc atcaacggcg tatattcatg
     3781 aatggtgaaa tctggcatct ccgggcgatg gaattccggc atcagcgcca gttggcgttg
     3841 tagatgacct gacaccggaa tataaccctg ctcggaacgc tgcatcccga tggtcccgcc
     3901 cgtgtaggcg acgtaaattg atttcttttg catgatattg atatgaatga tatcgaaaga
     3961 gcaaaagtat atacgtgagg caggaggagc gcacctgata ctatgcggat aaggaaatat
     4021 ggggcgagac agcgggaaga ggattcgtcg aggtaaaaaa taatgtggcc gacgatctgt
     4081 cggccactca gtacaagact taacgcacgt tggcgcaggt caggcaaaac gcataacggt
     4141 tttgtgggtc gttaaacgcg gccagcttgt cgctttcact tttaacggta gaggctaccg
     4201 aggcaagcgg tgcaggtaac atggcctgga acgcatctgg caacattgcc cggacagaac
     4261 cagacatgtt gtccatcact ttgtcgaaga aggtcggttc atcaacgtag tattccagat
     4321 gccactgttt cacttttgcg agctctgctg ctttggcaac cgcatcatcg aaatccccga
     4381 gactatcgac cagcccgtta gcttttgcat cctgaccggt ccagacgtgg ccctgggcga
     4441 ttttatcaat ttgctccggc gtcgaatgac gcgcatcagc aaccagcgtg ataaagcgtt
     4501 tatagccatt ctcaatgctt aattgcatca tctgctgcgc ttccggcggc agtgccctgg
     4561 tgatagaaac atccgccagc ggtgaagttg agacaccatc agtatgaaca ccaatcgaat
     4621 ccagactatt ttctacggtg gtgatcacgc cgaagatacc gatagaaccg gtcagggtgc
     4681 tggggttagc cacaatgtaa ttagctggcg tggaaatcca gtaaccacca gatgccgcca
     4741 tgccgcccat cgatacaacc acaggcttac ccgctgcccg ggctgctgcc agttcagcgc
     4801 gaatcacttc agacgcggta acgctgccgc ctgggctatt aacacgcagg acaatcgctt
     4861 tcactttcgg gtcaaggcga gcgtcgcgga tttgtgccgc agtggtatca ccgccaacat
     4921 tcccctgagt ttcctcgcca tccataattg cgccattagc gaagacgaca ccgatgctgt
     4981 caccggtatc tgccggcgtt ttcaatgcgt aatcgtaata actgatggcg cgataatttt
     5041 tatcagtctt actccagccg aactctttgg tcagagcttt ttcgatttcg gcactcgatg
     5101 ccagtgcatc gaccagcttg ttttccagtg catatttcgc ggtatcgcca ccggttttgg
     5161 ttaaaccctc aagcaaccct tgcgcgctag ggaatacctg ctgagcaggg atctgccggt
     5221 tagcggcaac agtattcaga tagttttgcc acagctcacc aatccagcgg ctgtcagctt
     5281 cgcgggctgc cggtgacata tcatcacgaa taaacggttc aacggcagat ttatacgtac
     5341 ccacgcggaa cacatgggtg gaaactttca gcttatccag caacgatttg tagtacagac
     5401 cgttggtagc aaaaccgtgc agatcaacca cgccttgcgg tgacagccaa attttattgg
     5461 cgaaactggc gagataatat tgcccctggc tgtagttctc gccaacggca taaaccggtt
     5521 tcccgctgtc acgaaactct ttcagggctt tgccgatgta ctgcatagac ggctggtcgc
     5581 cgcctgcgaa gtttttcaga tccatcacga taccggtgat attgtggtcg tccttcgcct
     5641 ggcgaatggt gttgacgata tcaaacagtg agttttcctg cagacgatcg gaactggcac
     5701 caagcagctg gcggcttaat ttactaaacc gctgagaact gtcgggttta tcgacgatca
     5761 caccggaaat gtccagcagc agtgcgccac gactggccgt ttctttcgaa tcaccaccac
     5821 tgacctgcat ccagatcccc acaccaacca gtacgaggaa aataaagaac aggttaagta
     5881 ccatttcacg gacgaaattc agcagacgcc acgtccattt aaaaaatccg gcaataaatc
     5941 gccaaagggt tcgcatgtat tctcccaact taaggtcaca cctgtcacag gcgcaataga
     6001 tgtggttatc gtaatgaccc aaccgacact tgtcagcagg aattgcggac tgtgctgtaa
     6061 caaaatccac tctcgtgtta attttgtgaa taaatatcac gacaggagtt aatcaaatgg
     6121 atgcactcga actattgatc aatcgccgta gcgcctcccg cctggctgaa cccgcgccaa
     6181 cgggtgaaca actgcaaaac atcctgcgtg cgggtatgcg tgcgccggac cataagtcca
     6241 tgcaaccgtg gcattttttt gtgattgaag gggaagggcg cgagcgtttc agcgccgtac
     6301 tggaacaggg ggcgattgct gccggtagtg atgacaaagc tatcgacaaa gcccgtaatg
     6361 cgccgttccg cgcaccgctc atcatcacgg tggtggcgaa atgcgaagaa aatcataaag
     6421 tcccgcgatg ggaacaggaa atgtctgccg gatgcgcggt catggcgatg caaatggcag
     6481 ccgttgccca gggatttggc ggcatctggc gcagtggcgc attaactgaa agtccggtag
     6541 tgcgtgaagc attcggttgc cgtgagcagg ataaaattgt cggttttctc tacctcggca
     6601 cgccgcagtt gaaagcatct acgtcgatta acgtcccgga cccgacgccg tttgtaactt
     6661 atttctgata attgtcgcga aactgtctgg attgtgagca aaggcgcagg aattcagaca
     6721 ctctcactta tcacttcacg gaatgagggt taccataccc ggattgcatt gacaggagat
     6781 gtccatgagc gagaactcga ttcgtttgac ccaatacagc cacggagctg gttgcggctg
     6841 taaaatttcc ccaaaagtgt tggaaaccat cctgcacagt gagcaggcga agtttgttga
     6901 tccgaatttg cttgtgggta atgagacccg cgacgatgcg gcggtgtacg atctgggcaa
     6961 tggcaccagc gttatcagta ccaccgactt ctttatgccg atcgttgata atcctttcga
     7021 ttttggccgc attgcggcga ctaacgccat cagcgatatc ttcgcgatgg ggggcaaacc
     7081 gattatggcg attgcgatcc tcggctggcc gattaacaaa ctttccccgg aaattgcccg
     7141 cgaagtaacc gaaggtggac gctatgcatg ccgtcaggcg ggtattgcgc tggctggcgg
     7201 tcactccatc gatgcgccgg agccgatttt tggtctggcg gtaacgggga tcgtaccgac
     7261 cgagcgggtg aagaaaaaca gcaccgcaca agccggatgc aaactgttcc tgacgaaacc
     7321 gctggggatc ggcgttctta ccacggctga gaaaaaatca ttgctgaaac cagaacatca
     7381 gggactagcg acggaagtga tgtgccggat gaacatcgca ggcgcgtcct ttgccaacat
     7441 cgaaggcgta aaagcgatga ccgacgttac gggctttggt ctgctgggcc atttgagcga
     7501 aatgtgtcag ggcgcaggtg tgcaggcgcg cgttgactat gacgcgatcc caaaattgcc
     7561 gggggttgaa gagtacatta agttgggcgc agtgcctggc ggcaccgaac gtaactttgc
     7621 cagctacggt catctgatgg gtgaaatgcc gcgtgaagtg cgcgatttgc tgtgcgatcc
     7681 gcaaacttct ggcggtttgc tgctggcggt catgccggaa gcagaaaatg aggtcaaagc
     7741 tacagccgcc gagtttggca ttgaactgac ggcaattggc gaactggtgc cagcgcgcgg
     7801 cggtcgtgcc atggttgaga ttcgttaatt caatgcggtt gtttattgcc gaaaaaccga
     7861 gtctggcgcg cgccattgct gatgtcctgc ccaaaccgca ccggaaaggc gatggcttta
     7921 tcgagtgcgg taatggtcag gtggtgacct ggtgtatcgg tcacctgctt gagcaggcgc
     7981 agccagacgc ctacgacagc cgctatgcgc gctggaatct tgcggatttg ccgattgtcc
     8041 cggaaaagtg gcaattacag ccccgaccct ccgtgaccaa acaacttaac gtcatcaaac
     8101 gcttcctgca tgaagccagc gaaatcgttc acgccgggga cccggatcgt gaagggcaac
     8161 tgctggtgga tgaagtgctg gactatctgc aactggcacc ggaaaagcgc cagcaggtgc
     8221 agcgttgctt gataaacgac ctgaacccgc aggcggttga gcgggcgatc gaccgtcttc
     8281 ggtccaacag tgagtttgtg ccattgtgcg tttctgcgct ggcgcgagcg cgtgccgact
     8341 ggctgtacgg catcaatatg acccgtgcgt ataccattct cggtcgcaat gccggttatc
     8401 agggcgtact ttccgtggga cgcgtgcaga cacccgtgct cgggctggtg gtgcgccgcg
     8461 atgaagagat tgaaaacttc gtggcgaaag acttctttga agtcaaagca catatcgtga
     8521 cacctgccga tgagcggttt accgctatct ggcaaccgag cgaagcgtgt gaaccgtacc
     8581 aggatgaaga agggcgcttg ttacatcgtc cactggcgga gcatgtggtt aaccgcatta
     8641 gtggtcaacc agcaattgtc accagctata acgataaacg ggaatcagaa tccgcgccgc
     8701 tgcctttttc gctttcggcg ttgcagattg aagcggcaaa acgctttggt ctgagcgcgc
     8761 agaacgtgct tgatatctgc cagaagctgt acgaaacgca caagctaatc acttatccgc
     8821 gttccgattg tcgctatttg ccagaagaac attttgccgg acgccacgcg gtgatgaatg
     8881 ccatcagcgt tcatgcacca gatctgttgc cgcaaccagt ggtagatcca gatatacgca
     8941 accgctgttg ggatgacaaa aaggtcgatg cacaccacgc catcatcccg accgcacgga
     9001 gttctgcgat caacctgacg gagaacgaag cgaaggtcta taacctgatt gcccgtcagt
     9061 acttgatgca gttctgcccg gatgcggtgt tccgcaagtg tgttatcgaa ctggacattg
     9121 ccaaaggcaa atttgtcgct aaagcgcgtt ttcttgctga agcaggctgg cgcacgctgt
     9181 taggcagcaa agagcgcgat gaagaaaacg acggtacgcc attgcctgtg gtggcgaaag
     9241 gcgatgagtt gctgtgtgaa aaaggtgaag tggtagagcg gcaaacccag ccgccgcgcc
     9301 attttaccga tgcaacactg ctttcggcga tgaccgggat cgcgcgcttt gtgcaggaca
     9361 aagatctgaa aaagatcctt cgtgcgaccg atggtctggg gacagaagca acccgtgccg
     9421 ggattattga actgctgttc aagcgtggtt ttctgaccaa aaaagggcgc tatatccact
     9481 ctaccgacgc cggaaaagcg ttattccatt cgctgccaga aatggcgacg cgaccggaca
     9541 tgaccgcgca ctgggaatcg gtgctgacgc aaatcagcga aaagcagtgt cgctatcagg
     9601 actttatgca gccgctggtg gggacgctat atcagcttat tgatcaagcc aaacgtacgc
     9661 cggtgcggca gtttcgcggc attgtggctc cgggcagtgg tggcagtgct gataagaaaa
     9721 aggctgcacc gcgtaaacgt agtgcgaaaa aaagtccgcc agcagatgaa gtcggaagcg
     9781 gggcgatagc gtaagcgagt gaatctttcg tgctattcga gtcatattct gaaatatcca
     9841 gcgaatcaag aaaattcgtt ggatattttt ttgcatggat aaaattatcg cctctaaagt
     9901 atgtaataac agggaatgtg atgaaaaaag ttttattaca aaaccatcct gggagcgaga
     9961 agtattcttt taatggctgg gaaatattta atagtaattt tgaacggatg attaaagaaa
    10021 ataaggccat gctgctttgt aagtgggggt tttatttaac atgtgttgtc gctgtaatgt
    10081 ttgtgttcgc agcgataaca tccaacggtt tgaatgaaag aggcctgatt accgcgggat
    10141 gctcttttct ttatctatta attatgatgg ggcttattgt tcgggccggt tttaaagcaa
    10201 aaaaagaaca actgcattat tatcaggcta aaggtattga gccgctcagt atcgagaagt
    10261 tacaggcgct acaattgatc acaccttatc gattctatca taagcaatgg tctgaaacgc
    10321 tggagttctg gccgcgaaag cctgaacctg gcaaagatac cttccaatat cat
//
LOCUS       DAJVUJ010000149        10330 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.149, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000149 DAJVUJ010000000
VERSION     DAJVUJ010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10330
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.149"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            121..987
                     /gene="folD"
                     /locus_tag="OQQ48_003377"
     CDS             121..987
                     /gene="folD"
                     /locus_tag="OQQ48_003377"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HCS1954030.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            989..1201
                     /gene="ybcJ"
                     /locus_tag="OQQ48_003378"
     CDS             989..1201
                     /gene="ybcJ"
                     /locus_tag="OQQ48_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HCS1954031.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            1309..1830
                     /locus_tag="OQQ48_003379"
     CDS             1309..1830
                     /locus_tag="OQQ48_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCS1954032.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTPYGHQVIISELMWVWLP
                     GMVLMGMLWWRRR"
     gene            complement(1866..3251)
                     /gene="cysS"
                     /locus_tag="OQQ48_003380"
     CDS             complement(1866..3251)
                     /gene="cysS"
                     /locus_tag="OQQ48_003380"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCS1954033.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQTRAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            3425..3919
                     /gene="ppiB"
                     /locus_tag="OQQ48_003381"
     CDS             3425..3919
                     /gene="ppiB"
                     /locus_tag="OQQ48_003381"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HCS1954034.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            3922..4644
                     /gene="lpxH"
                     /locus_tag="OQQ48_003382"
     CDS             3922..4644
                     /gene="lpxH"
                     /locus_tag="OQQ48_003382"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HCS1954035.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            4762..5271
                     /gene="purE"
                     /locus_tag="OQQ48_003383"
     CDS             4762..5271
                     /gene="purE"
                     /locus_tag="OQQ48_003383"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCS1954036.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            5268..6335
                     /gene="purK"
                     /locus_tag="OQQ48_003384"
     CDS             5268..6335
                     /gene="purK"
                     /locus_tag="OQQ48_003384"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCS1954037.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETVLTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YMGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDANYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(6472..7363)
                     /locus_tag="OQQ48_003385"
                     /pseudo
     CDS             complement(6472..7363)
                     /locus_tag="OQQ48_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
     gene            complement(7360..8175)
                     /locus_tag="OQQ48_003386"
     CDS             complement(7360..8175)
                     /locus_tag="OQQ48_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HCS1954038.1"
                     /translation="MTIIHPLLASSSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     SHPQPLAAAWMQRAEETGLFGPLAMAASDPLTAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARAGRPFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(8186..9445)
                     /locus_tag="OQQ48_003387"
     CDS             complement(8186..9445)
                     /locus_tag="OQQ48_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HCS1954039.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPAFSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGGCLFEGWAKDEAQALAMLEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMEHGIDLTAMMAQGITMGDEFHQRNIASSALLMRTLAPQIARLDHDKQHIAEV
                     IDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            complement(9455..10330)
                     /gene="fdrA"
                     /locus_tag="OQQ48_003388"
                     /pseudo
     CDS             complement(9455..10330)
                     /gene="fdrA"
                     /locus_tag="OQQ48_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415051.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
BASE COUNT         2272 a         2937 c         2774 g         2347 t
ORIGIN      
        1 aaccttcgca agaggtcgct tcacgcgata aatctgaaac gaaacctgac agcgcgcccc
       61 gcttctgaca aaataggcgc atccccttcg acctacgtaa cagatggaat cctctctctg
      121 atggcagcaa agattattga cggtaaaacg attgcgcagc aggtgcgctc tgaagttgct
      181 caaaaagttc aggcgcgtat tgcagccgga ctgcgggcac caggactggc cgttgtgctg
      241 gtgggtagta accctgcatc gcaaatttat gtcgcaagca aacgcaaggc ttgtgaagaa
      301 gtcgggttcg tctcccgctc ttatgacctc ccggaaacca ccagcgaagc ggagctgttg
      361 gagcttatcg atgcgctgaa tgccgacaac accatcgatg gcattctggt tcaactgccg
      421 ttaccggcgg gtattgataa cgtcaaagtg ctggaacgta ttcatccgga caaagacgtg
      481 gacggtttcc atccttataa cgtcggtcgt ctgtgccagc gcgcgccgcg tctgcgtccc
      541 tgcaccccgc gcggtatcgt cacgctgctt gagcgttaca acattgatac cttcggcctt
      601 aacgccgtgg tgattggcgc atcgaatatc gtcggtcgtc caatgagcat ggaactgttg
      661 ctcgcaggtt gcaccactac cgtgactcac cgtttcacta aaaatctgcg tcaccacgta
      721 gaaaatgccg atctgttgat cgttgccgtc ggcaagccgg gctttattcc tggtgactgg
      781 atcaaagaag gcgcaattgt gattgatgtc ggcatcaacc gtctggaaaa tggcaaagtt
      841 gtgggcgacg tcgtgtttga agacgcggct aaacgcgcct catacattac gcctgttccg
      901 ggcggcgttg gcccgatgac ggttgccacg ctgattgaaa acacgcttca ggcgtgcgtt
      961 gaatatcatg atccacaggg tgagtaacat ggcgacattt tctttaggta aacatccgca
     1021 cgttgagctg tgcgatttgc tgaaactgga aggctggagc gaaagcggcg cgcaggcgaa
     1081 aatcgcgatt gccgaaggcc aggtgaaagt cgacggtgcg gttgaaacgc gcaaacgctg
     1141 caaaatcgtc gccggtcaga cagtgagttt tgcaggtcac agcgtacagg ttgttgcctg
     1201 atccctttcc cggcctgtat caacaggccg gttctctttt cagacttttg ccctctacgc
     1261 cccgttcatc catgctgata gcatcgacat tttgccgaag cgaccatcat gccaaccgtt
     1321 attacgcacg ccgccgttcc cctatgcatt ggtttagggt tgggttcgaa agtcatccct
     1381 ccgcgtctgt tatttgccgg aatcatcctg gcgatgctgc ccgacgccga cgtattgtcg
     1441 tttaaatttg gcgttgctta cggcaatgtt tttggtcatc gcgggtttac ccattcgctg
     1501 gtgtttgcgt ttgttgtccc gctgttatgt gtgctgattg gacgccgatg gttcagggct
     1561 gggcttattc gctgctggct gtttttaacc gtctcgttgc tatcgcacag cttgctggat
     1621 tcggtaacca ctggcggtaa gggcgttggc tggctgtggc cgtggtcaga tgaacgcttt
     1681 ttcgctccct ggcaggtgat taaagtcgcg ccgtttgcat tgtctcgtta caccacgccg
     1741 tacgggcatc aggtgataat ttctgaattg atgtgggtgt ggttgccggg gatggtgctt
     1801 atgggaatgt tgtggtggcg caggcgataa ccggatgcga aaactcgcat ccggcaacag
     1861 cgcaattact tacgacgcca ggtggtccct tgcgggccat cttccagcac gatccccatc
     1921 tcgttaagac ggtcacgcgc cgcatctgcc gccgcccagt ctttcgcttt acgggcatcc
     1981 agacgctgtt gaattaacgc ttcaatctca gccacttcgc tgtcgtctgc ctgcgcgccg
     2041 ctttgcagga acgcttccgg ttcttgctcc agcaggccca atacagcgga aagtttacgc
     2101 aggtgagatg ccattgcatt cgccgctgcc atatcttctg ctttcagacg gtttacttca
     2161 cgcgccatat caaacagtac ggaataggct tccggggtgt tgaaatcgtc gtccatcgct
     2221 tcaataaagc gcgcttcaaa cgcttcgcca ccggcaggcg caacggtttt atctgtgccg
     2281 cgcagcgcag tgtagagacg ctccagcgcc gcacgcgtct gcttcaggtt ctcttcgctg
     2341 tagttcaact ggctgcgata gtggcccgac atcaggaagt aacgcacggt ttccgcgtcg
     2401 tagtatttca gcacatcgcg cacggtaaag aagttaccca gcgatttgga catcttctcg
     2461 cggtcaacca tcaccattcc ggagtgcatc cagtagttca catactgacc atcatgggca
     2521 caggtggact gcgcgatttc gttctcgtgg tgcgggaaca tcaggtctga accgccgccg
     2581 tggatatcaa agtggttacc cagctgcttg cagttcatgg ccgaacattc aatgtgccag
     2641 ccaggacgac ccgcgcccca cggagacggc cagctcggtt cgccctcttt cgacatcttc
     2701 cacagaacga agtccatcgg gttgcgtttg tcatcaacca cgtcaacgcg cgcgccagcc
     2761 tgcagctggt cgagatcctg acgcgacagc acgccataag tcggatcggt cgggacgtcg
     2821 aacatcacgt cgccgttgtc agccacataa gcgtgacctt tggcgatcag ttgttcagtg
     2881 agttcaataa tttctgcgat atggtgcgtc gcgcgcggct ccatatccgg gcgcagaatg
     2941 ttcaaagcat caaaatcttt gtgcatttcg gcgatcatgc gatccaccag cgccacaaag
     3001 ctttcgccat tttcattggc gcgtttgatg attttgtcgt cgatatcggt aatgttgcgc
     3061 acatacttca gcttatagcc gaggaaacgc agatagcgcg caaccacgtc aaaagcaaca
     3121 aaggtacgcc cgtgaccgat atgacagaga tcgtaaacgg tgattccaca cacgtacatg
     3181 ccgacttccc cggcgtgaat aggcttaaat tcctcttttt ggcgtgtcag agtattgaag
     3241 atttttagca tcgaagattc cgtttagaca tgtgtgggta attgagttgc gtataatacc
     3301 catatttccc gcgcgaatca gcatacattg cgtgatgatc cgatcgtgcg gttatgctat
     3361 aacaccaccc tatatatgac ccgaaccggg ttgaagcacc aatcaaacgg aacaggatgc
     3421 aaaaatggtt actttccaca ccaatcacgg cgatattgtc atcaaaactt ttgacgataa
     3481 agcacctgaa acagttaaaa acttcctgga ctactgccgc gaaggttttt acaacaacac
     3541 cattttccac cgtgttatca acggctttat gattcagggc ggcggttttg aaccgggcat
     3601 gaaacaaaaa gccaccaaag aaccgatcaa aaacgaagcc aacaacggtc tgaaaaatac
     3661 ccgtggtacg ctggcaatgg cccgtactca ggctccgcac tctgcaactg cacagttctt
     3721 catcaacgtg gttgataacg acttcctgaa cttctctggc gaaagcctgc aaggttgggg
     3781 ctactgcgtg tttgctgaag tggttgaagg catggacgtg gtagacaaaa tcaaaggtgt
     3841 tgcaaccggt cgtagcggta tgcaccagga cgtgccaaaa gaagacgtta tcattgaaag
     3901 cgtgaccgtt agcgagtaat cgtggcgaca ctctttattg cagatcttca tctctgcgtg
     3961 gaagaaccgg cgatcaccgc cggttttctg cgttttttag cgggggaagc tcgcaaggcc
     4021 gacgcgctgt atattcttgg cgatctgttt gaagcgtgga ttggcgacga cgatcccaat
     4081 ccactccatc gccagatggc ggcagcgatc aaagcggtgt ccgattccgg cgttccctgt
     4141 tatttcattc atggcaaccg tgattttctg ctcggcaaac gctttgcccg tgaaagtggc
     4201 atgacgttat tgccggaaga aaaggtgctc gaactttatg gccgccgggt gttgattatg
     4261 catggcgaca cgctgtgcac cgatgacgcg ggttatcagg cttttcgcgc caaagtccac
     4321 aaaccctggc tgcaaatgct attcctcgcc ctgccgttgt ttgtgcgcaa acgcattgcc
     4381 gcgcgaatgc gcgcgaacag caaagaagcc aacagcagta aatcgctggc gatcatggac
     4441 gttaaccaaa acgcagtggt cagtgcgatg gaaaaacatc aggtgcaatg gctgatccac
     4501 gggcataccc atcgcccggc ggtgcatgaa cttatcgcca accagcaacc tgcttttcgc
     4561 gtggtactgg gtgcctggca tacggaaggt tcgatggtga aagtcacggc ggatgacgtt
     4621 gagctgattc attttccgtt ttaaaaaaac cacaactttg ctgatttcac agccacgcaa
     4681 ccgttttcct tgctctcttt ccgtgctatt ctctgtgccc tctaaagccg agagttgtgc
     4741 accacaggag ttttaagacg catgtcttcc cgcaataatc cggcgcgtgt cgccatcgtg
     4801 atggggtcca aaagcgactg ggctaccatg cagttcgccg ccgaaatctt cgaaatcctg
     4861 aatgtcccgc accacgttga agtggtttct gctcaccgca cccccgataa actgttcagc
     4921 ttcgccgaaa gcgccgaaga gaacggttat caggtgatta ttgcgggcgc aggtggcgca
     4981 gcgcacctgc caggcatgat tgccgccaaa acgctggtgc cggtgctggg cgtgccagta
     5041 cagagcgccg cactgagcgg tgtcgatagc ctctactcca tcgtacaaat gccgcgcggc
     5101 attccggtgg gtacgctggc gattggtaaa gctggcgcgg caaacgcggc attactggca
     5161 gcacaaattc tcgcgactca cgataaagag ctacaccagc gtctgaatga ctggcgcaaa
     5221 gcccagaccg acgaagtgct ggaaaatccg gacccgcgag gtgcggcatg aaacaggttt
     5281 gcgtcctcgg taacgggcag ttaggccgta tgctgcgtca ggcaggcgaa ccgttaggca
     5341 ttgctgtctg gccggtcggg ctggacgctg aaccggcggc ggtgcctttt caacaaagcg
     5401 tgattaccgc tgagatcgaa cgctggccgg aaaccgtatt aacccgcgag ctggcgcgtc
     5461 atccggcctt tgtgaaccgc gatgtgttcc cgattattgc tgaccgtctg actcagaagc
     5521 agcttttcga taagctccac ctgccgaccg cgccgtggca gttacttgcc gatcgcagcg
     5581 agtggcctgc ggtgtttgat cgtttaggtg aactggcgat tgttaagcgt cgcactggtg
     5641 gctatgacgg tcgcggtcaa tggcgtttac gcgccaatga aaccgaacag ttaccggcag
     5701 agtgttacgg cgaatgtatt gtcgagcagg gcattaactt ctctggtgaa gtgtcgctgg
     5761 ttggcgcgcg cggctttgat ggcagcaccg tgttttatcc gctgacgcat aacctgcatc
     5821 aggacggtat tttgcgcacc agcgtcgctt ttccgcaggc caacgcacag cagcaggcgc
     5881 aagccgaaga gatgctgtcg gcgattatgc aggagctggg ctatatgggc gtgatggcga
     5941 tggagtgttt tgtcaccccg caaggtctgc tgatcaacga actggctccg cgtgtgcata
     6001 acagcggtca ctggacacaa aacggtgcca gcatcagcca gtttgagctg catctgcggg
     6061 cgattaccga tctgccgtta ccgcaaccag tagtgaataa tccgtcggtg atgatcaatc
     6121 tgattggtag cgatgcgaat tatgactggc tgaaattgcc gctggtgcat ctgcactggt
     6181 acgacaaaga agtccgtccg gggcgtaaag tggggcatct gaatttgacc gacagcgaca
     6241 catcgcgtct gactgcgacg ctggaagcct taatcccgct gctgccgcca gagtatgcca
     6301 gcggcgtgat ttgggcgcag agtaagttca gttaagcagg tgctcatttg attgccggat
     6361 gcggcgtaaa cgccttatcc ggcttaccgc tccggtaccc attgtaggcc tgataagacg
     6421 cgtcaagcgt cgaatcaggc atggtgcacc gattgccgga tgctctcatc actacagcga
     6481 aatacaggtc cccgcttcgc ccgccagcgt ctcttcaatt cgcgataacg ccccaatcca
     6541 cgcgggttta ctacggcttc tgacataacc actcaccgcc gttaccttcg gccccatcga
     6601 accatcggct ttggcaaatg gcgctaactc atccggtgtg gcatggcgaa tggcacgttg
     6661 ctgcggcgtt ccccagtttt catataccgc atcagcatcg gtgaggatca ccagtccatc
     6721 ggcattaatc tgctcggcga gcaacgcagt ggcgagatct ttatcaatca ccgcttcact
     6781 ccctgcccca tcttccgtca caggcacacc gccgccgcca ctgcaaatca ccacatgccc
     6841 ctctttgagc aacaactcga tggcttcgct gtcgagaatt ttacgcggtt gcggagacgc
     6901 caccacccgg cgcaaatatt taccatcacg tttcatctgc cagccgtaag ccgcttccag
     6961 tgcctcttgt tcttctggct gataaaccgg accaataaat ttctctggct gcaaaaacgc
     7021 cggatcatca ggcgaaacct caatgcgcgt cagcaccgtc gtaacaggcg gtatctgcgg
     7081 ctgtgcgctc aagctctgcg ccagcatata gcctcatccc ctggctttcc gccaccagca
     7141 catccagcgg atacggttct acctctttcc acgccagatt ctgtaacgcc agcagcccca
     7201 cctgcggccc gttgccgtga acaatcgcca gccgataaga acgggccagg cgtgccagcg
     7261 cgggtacagc actggcgata ttgcgatatt gattttccgc cgtcagcgcc tcaccgcgct
     7321 ggagtaaggc gttgccccca agagctacaa ccagtgtttt catggttttc cttgtaataa
     7381 ttgttggcca agccagaacc ccagcagcgt atctgcccct gacgtatgcc ccagcgccag
     7441 cagcgaatca atcgcaacag cggtacgttt cgggcaactc agagcatgga caaagtgcag
     7501 gagtggcgag gcgaaatatc cttgcgcggc ataacgtaaa taactgacgc tcaccgctgt
     7561 ggtaacgagt tgaagattgt cggaacaggc aaaaaacggg cgaccggcgc gcgcatctaa
     7621 agcgccataa taccaagccg ccagcagcat tccgctcaac gtgtcatcat gacttggcgt
     7681 taatccgggg cctttaccca gccagtgccg ccagtcggtc ttaacgccat tgagcgcggc
     7741 ctgaaaacag tgacgaaact ggcgtaattc agcggtcagc ggatcgcttg ccgccatcgc
     7801 cagtggtccg aaaagtccgg tttcctccgc gcgttgcatc catgcagctg caagcggttg
     7861 aggatgcgaa ggcggcgtaa tacgcagcaa acaataacgc tgtggctgtt taaccgtgaa
     7921 acgcccgagg cgaatccctt gagccacaac ctgtggtcgt tcattgccgc ataatccacc
     7981 gcataacgca tcgaattgcg cgcggcgaag cacccatcct ccggggccga aaccactacc
     8041 ctgacgatgc agcgttaaca gttcgccgct ttccgtcatc aggttaatcg cccgccgcca
     8101 cactcccgct aaccgccagg actggcgata attcggtgcg ctactgctgg caagcagagg
     8161 atggatgatc gtcattgcgc gttcctcaac caatccccat gctttcagcc agtgcttcca
     8221 gcgcctgttc aaagcacgcc agcggtgccc gcacggtgcc tgcgccaatc tgcccgatcc
     8281 ccgcctcttt atgggcgata ccggtattga tgagtggcgt aatgccggtt tctaccacgc
     8341 gacgaatatc cagccccagg cacgcgccct gaaaatccca gcctgggatc tgcaactgca
     8401 tattgcgttc aaggtaaatt tccgccatct cttcagatac cgccctggcc gcttccatgc
     8461 cacccgcacc gacaaagcgc gttacgccag gcgctgcgat cattgccgca cctccgatac
     8521 caaaggtttc ggtaatcgcg ctatcgccca tatccgggtt cgcctgctcc tgcgagaagc
     8581 cggtgaaaaa cagaccttgc ggagtgttta caggcgcagt aaaccagcgt tcacccagcc
     8641 cgcttacccg aatcccgaac atattgccgt tgcgggtcat agccgtaacg atgctgcctg
     8701 cgcggatcat cgcgccagca tccatcgccg ccttgcagta ggccatcgcg aggttgagga
     8761 agaactgatc ggtcacgctg aggaaatcga tcacttccgc aatgtgctgt ttatcatgat
     8821 cgaggcgagc aatttgtggg gccagcgtac gcattaacag tgctgaggaa gcaatattgc
     8881 gttgatggaa ctcatcgccc atcgtaatgc cctgcgccat catcgccgtg agatcgatac
     8941 cgtgctccat gcgccccagc gccgcgctta atactggcat taacacatcg cgcatccagc
     9001 gatggcgagt caggacatct tcgccgtaag cgccaaaacg catcactttg ccgatacctt
     9061 cgttgaggtt gcagtacgcc cggttgccgt cggtcacgtt ctcgaccacc agcatcggca
     9121 tactggcaga agtaatgccg cccattggcc cgacggcatt cacatggtga caaggaatga
     9181 agttcacttc cccctgctcc agcatcgcca gtgcctgcgc ttcatctttc gcccagcctt
     9241 cgaacagaca tccgcccacg cacgcccctt tcatgggtcc ggtcatctcc tgccagcgca
     9301 ttggcggccc ggcgtgaagc agtgttttgc cctcgtttag ttcgctgata agtgaaaaag
     9361 ccggttgcac atccagccag tgtggacgag cacgacgaat ttgttcgata accgcagcat
     9421 tggcttgcgc cactgatgta aacatgggaa ccccttattg caaacgttct aataaacgag
     9481 ccagtttttt attgccgccg gcgactggcg accattggta atgcacaacc ggtttgctgg
     9541 cgctttgtag ctccagcgca aagctgcgta atccgatgtt aatcacggcg acgttttcca
     9601 gtaatgacgg tgtatgttgc tgtgcggcag gcgagagcgg ataaattaac gctgccgcca
     9661 gcaaggtggc ttccggcagc gaactcacta ccgcgatccc cgcatcttcc agcgtggcga
     9721 tttgctgcga gcggcattgc gggtcacgtt cagtgcctgt caccgtggca atggcataca
     9781 gtggttgatt atctgaacgc gcggcacagg ctttttgcta ggcgcttacc agcgaggcgg
     9841 caggatcggc agtcgcaccg aagccaatca cgacatcaag tagcaatacg cgcacttgtg
     9901 gtttagcgcc gagatcggca attaactggt tgcgtaaggc tgggtcgatc atcggatggg
     9961 gacgcccgac ggtgtagaaa tcatcgccga ggtcaataat ctggtgacca tcggcgtcga
    10021 gcatcatgcc gtgatgatgg gtatcgtcgg cttccacgcc aaggtgtcca gcaagtaatc
    10081 ccgccgcttc ggcagccagc gtaccgccgg tatacaaacc gcaaataaat ccgctgctga
    10141 caggcgctat tgcgttacgt cgcgccgtga cgcgtgaaag caggcaagcc aggcgtgcgg
    10201 cctcatccag cgaggaggca aaccagacat tctcgtcgcg ggccaccgcc ggggtataac
    10261 ctaaaaacag cgccaccatc ggtttgccgg ttgctttcat ggcattaaca attttcagac
    10321 gcacagcttc
//
LOCUS       DAJVUJ010000150        10320 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.150, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000150 DAJVUJ010000000
VERSION     DAJVUJ010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10320
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.150"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..275
                     /locus_tag="OQQ48_003389"
     CDS             <1..275
                     /locus_tag="OQQ48_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HCS1954040.1"
                     /translation="ALVVAAAMGLSSAAFAAETATTTKAAPAKTTHHKKQHKAAPAQK
                     AQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHSHQQPAKPAAQPAA"
     gene            368..451
                     /locus_tag="OQQ48_003390"
     CDS             368..451
                     /locus_tag="OQQ48_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1954041.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            551..1372
                     /locus_tag="OQQ48_003391"
     CDS             551..1372
                     /locus_tag="OQQ48_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HCS1954042.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTAQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVVLRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTNDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(1411..1740)
                     /gene="mdtI"
                     /locus_tag="OQQ48_003392"
     CDS             complement(1411..1740)
                     /gene="mdtI"
                     /locus_tag="OQQ48_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HCS1954043.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSTLSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(1727..2092)
                     /gene="mdtJ"
                     /locus_tag="OQQ48_003393"
     CDS             complement(1727..2092)
                     /gene="mdtJ"
                     /locus_tag="OQQ48_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HCS1954044.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            2504..2647
                     /locus_tag="OQQ48_003394"
                     /pseudo
     CDS             2504..2647
                     /locus_tag="OQQ48_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pheromone autoinducer 2 transporter"
     gene            complement(3172..3453)
                     /locus_tag="OQQ48_003395"
     CDS             complement(3172..3453)
                     /locus_tag="OQQ48_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1954045.1"
                     /translation="MTEAEITGLIRRVAGISQQADEQATQPDSVTAENYARVVAEVMR
                     RDGIELNGVDMRNIRTRVLELLAYRRRVEMYREKEKITYHWKKPERLRR"
     gene            complement(3467..5128)
                     /locus_tag="OQQ48_003396"
     CDS             complement(3467..5128)
                     /locus_tag="OQQ48_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCS1954046.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLKSPLYTFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDDARQMCLLSRPLRRRVNI
                     QAHKVIHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDENEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGTTPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKTIMLVSRHYLPEFQLQNPANKNRA
                     VYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGTFQAEHEDFAFDISDSHRQKLEEFSGV"
     gene            complement(5112..5468)
                     /locus_tag="OQQ48_003397"
     CDS             complement(5112..5468)
                     /locus_tag="OQQ48_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HCS1954047.1"
                     /translation="MARPPKAPAYLDDIAVKQWREKSRQLAERGDLTPADWSNLELYC
                     VNYSIYRKAVADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRR
                     KNPPETEEEDELDRLE"
     gene            complement(5591..5773)
                     /locus_tag="OQQ48_003398"
     CDS             complement(5591..5773)
                     /locus_tag="OQQ48_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1954048.1"
                     /translation="MTLMNEREQKRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRDREVMECFRNR"
     gene            complement(5757..6197)
                     /locus_tag="OQQ48_003399"
     CDS             complement(5757..6197)
                     /locus_tag="OQQ48_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707053.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCS1954049.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWEKYRAMYLSKNPLCVRCLEKGIYTPAVVVDHIIPINGGDDVLFWPEWNHQPL
                     CQACHNQKTKWLDPATKSKRAAGGFREEEERAANRNNWMYDADE"
     gene            complement(6197..6493)
                     /locus_tag="OQQ48_003400"
     CDS             complement(6197..6493)
                     /locus_tag="OQQ48_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HCS1954050.1"
                     /translation="MTEELITLEEVKLHCRIDGDEEDQLISGYIAASLEACQIHIGRR
                     FDDGLEFTPAIKIGCMMFIAHLYENRQLVADNAKTRVPMTIGALWTAYRDVGVY"
     gene            complement(6490..6828)
                     /locus_tag="OQQ48_003401"
     CDS             complement(6490..6828)
                     /locus_tag="OQQ48_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCS1954051.1"
                     /translation="MNIGRLRDRVTIQTLKQTRDITGEILETWEDGHTLWASVNMISS
                     KEAISSGAELAIGTVRIWIRYRKDINATSRIKVNTGPLAGRVLNIIGQPLPDAARTRL
                     EILCREGAEK"
     gene            complement(6825..8036)
                     /locus_tag="OQQ48_003402"
     CDS             complement(6825..8036)
                     /locus_tag="OQQ48_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCS1954052.1"
                     /translation="MWLFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLPAV
                     MNAVTVISEAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRHCLLNGNAYAVIQWGRDGFPVALHPYPPQSVNVEQTGEHNWRYCITDAYTGNTRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVASPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSGVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            complement(8038..8610)
                     /locus_tag="OQQ48_003403"
     CDS             complement(8038..8610)
                     /locus_tag="OQQ48_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HCS1954053.1"
                     /translation="MGEKIMKSMEIRSSEITTSASNTLTGYVVRWDKLSELLWGEFYE
                     KFQRGAFTEWLAVGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDTSTG
                     RDVIELVKRGDISGMSFGFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPRADNRRWWVDLAGV"
     gene            complement(8650..9807)
                     /locus_tag="OQQ48_003404"
     CDS             complement(8650..9807)
                     /locus_tag="OQQ48_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCS1954054.1"
                     /translation="MPRIIELRQQKTAIKNQMRDMLENAEKENRSLNDAEGAKFDELR
                     AKAESLDKDISRLEAIADEERSKPGKSSQTTDPAELRSYILTGETRALSTGVPADGGY
                     TVIPELNTEIMRMLADESTMRRICTVKKISSNEFKQLVSAGGVTVNHGEEGKAREQTS
                     TPQINEVSIKLYPVYAYPRTTQEIVDFSDVDILSWLTGEIGDTFTETEESDLVVGDGD
                     KKAKGFLSVPRAEKNDKERDFGTLQVIKPSESLAWTSADPLIDLKFALRKKYRKNAVW
                     VVNSTTAAKLQKVKNANGDYIWRDRLQAGDPDTLLGLPVEYLEFMPDNVIALGDFKRG
                     YYIVDHETGVRTRPDNLTEPGFIKIFTQKYLGGGVVDSNAIKILELPQDDD"
     gene            complement(10109..10312)
                     /locus_tag="OQQ48_003405"
     CDS             complement(10109..10312)
                     /locus_tag="OQQ48_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1954055.1"
                     /translation="MLKQRDKYHADNMETMSINDYRAFLETGALIEKDQHGFVRCALS
                     GEMLAVNPEQIDALIEFLKGIRD"
BASE COUNT         2386 a         2821 c         2463 g         2650 t
ORIGIN      
        1 tagctctggt tgttgccgct gctatgggtc tgtcttctgc cgcctttgct gcagagactg
       61 cgacgaccac caaagcagcg ccggcgaaaa cgacacatca taaaaaacag cataaagcag
      121 cacctgccca gaaagcgcag gcagctaaaa agcatcataa aaatacgaaa gctgaacaga
      181 aagcccctga acaaaaagcg caggcagcga agaaacacgc cgggaaacac agtcatcagc
      241 aaccggcaaa acctgctgca caacccgcag cgtaagtttt caacagtaat gctggcgcgc
      301 cccctcgcgc ctgaaaatta cggtgctaag cgggtaacgt ttagcaccgc ctttagcgga
      361 gggtagtatg gtgggccgtt atcgctttga gttcattctt atcatcctta ttttatgcgc
      421 actgattgcc gcccgttttt atctttcctg actgtagtta tctgatttta ctcccacttt
      481 catcccgtcc cgtctatagt atttatgagg gtttgctttt aataatcata attacccacc
      541 agagtgtgat atgcgtacaa ccattgctgt agtgttgggt gcaattagtt tgacgtctgc
      601 ttttgtgttt gcagataaac cagacgttgc caaatcggca aacgatgagg tcagcaccct
      661 gttttttggt catgatgatc gtgtgccagt gaatgacacg gcccaatcac cgtgggatgc
      721 ggttgggcaa ctggaaacgg ccagcggcaa tttatgtacg gcgacgctga ttgcacccaa
      781 tctggcatta acggcaggac actgtttatt gacacctcca aagggtaaag ccgataaagc
      841 agtggtgctg cgttttgtgt caaataaagg tctttggcgc tatgagatcc acgacataga
      901 aggccgcgtt gatccgacac tgggaaagcg gttaaaagca gatggggatg gttggattgt
      961 acctcccgca gccgcgccgt gggacttcgg attgattgtg ctacgtaatc ccccttctgg
     1021 cattacaccg ttgccgttat ttgagggaga taaagccgcg cttactgccg cattaaaatc
     1081 ggctggccgt aaagtaactc aggcgggata ccctgaagat catctcgata cgttgtacag
     1141 tcatcaaaac tgtgaagtga ctggctgggc gcaaacgtcg gtgatgtcac atcagtgcga
     1201 taccttgccg ggtgacagcg gttcgcctct gatgttgcat accaatgacg gctggcaatt
     1261 aattggggtg caaagttcgg ctcctgccgc gaaagatcgc tggcgcgccg ataaccgggc
     1321 catttctgtt accggttttc gcgacaagct ggatcaactg tcgcaaaaat aatgttcaaa
     1381 cgctgcccga ctgtgcgggc agcgtgttca tcaggcaagt ttcaccatga tcattccagc
     1441 caacagcaag accaggccaa tccagccttt acgatttaac cgttgaccaa acaagatcca
     1501 acctgcggct aacgtggcgg caataccaaa cccgccccac aatgcataag cgacagacaa
     1561 gtcgatccct ttaacggcct gagaaagcgt actaaaggca gccagcaccg ccgccaggga
     1621 gagcaagcca aatattttgc gacgaaagcc gtcagaaaat ttcaaaaaga cgttagcaac
     1681 gatttccagc acgattgcca atgccagcca ggcggcgtga acccattcaa actgcgccat
     1741 ggtttacctc cagttcaggt ttacgcgctt tacgggtacc tgattttatc aacacaatcc
     1801 cggcgaccag ggtggttaac ccggcaattt tcatcagcga taaactttcg tcgaataaca
     1861 aaacgctaaa caaggtaata aataaaatac cgataccttc ccacagcgca taagctacgc
     1921 ctaaggcgat ctttttaacg gcgaaagaga gaaatatata cgacagagaa atcatcacca
     1981 gcattaaaat aaagccgcca tttccctcac tgacgctcgc ccatttcatt gacagcgtac
     2041 cggtaatttc tgtagcaata gccaggccta ataaaatcca ataaatatac attgtccttc
     2101 tcctgcaaga gaattatttt aattttcgct taattcagcg aaattaaagt aaagtggtca
     2161 agccccgcgc atagggcaag cttaggcaga agaaaggact aaagcgcgtt gcgccagtgc
     2221 tgctcaccta cgagcaagat agtggatgag gtacaaagat gggtaaatgt tgaaaataag
     2281 gtcctgaaca aattgtccat aatattacaa ttatccgcag tgttgcttct cgtcatcgcg
     2341 gatgataatt gtcctcggta gttgaacacg cctgatttgt atcatagctt aagaattaac
     2401 tcaaaatatt ttcacttctt tacctgagcg gtttgatttt cgttatgatg acggagcgaa
     2461 aaagacatta ttattagcaa aggaagaaaa aacggggaca agcatggcaa agccgatcat
     2521 cacgctcaat ggcctaaaaa tcgtcattat gttgggaatg ctggtcatta ttctctgcgg
     2581 tatccgtttt gccgccgaga tcatcgtgcc gtttattctc gcattattta ttgctgttat
     2641 atataactta tttcacttaa cgcattgatt taattaaata atttgcacca caaaaagtgc
     2701 caaaaatcgc aaaatgacta caccagtgac tacaccgttc ggtgcactgt atgaaacaac
     2761 gtggaacaaa tagacacaag aaatatacag gcagatcatc tttccagggg gaagcgcacc
     2821 aattcatgag gggcgttgct gtcgatatgg ggatctccac ctacctggat ttttttccgg
     2881 ttaaacgtta atcaggctgg tgggctttac ctgttcggta ggagtggtca ttatgaccat
     2941 tcagacaatg accttaatca gaatttttcg ctaaaaaatg acgttaacca cgtcgtccgg
     3001 aaaacacaaa atcccgtcga tttttaatta acggttaaac cgtcttttgt cacggcgtgt
     3061 cactggctgt cactctccgt cacgattggg cgtggtgatt atcaccattc agaatctaaa
     3121 gggtgacgct tccggcctcc tcaaattgag gtttccgaaa atatcaacca gttaccgccg
     3181 caaccgctcc ggcttcttcc agtggtacgt tattttctcc ttctcccgat acatctccac
     3241 gcggcgacgg taggccagca actcaagaac tctggttcgt atgttgcgca tatccacgcc
     3301 attaagttct ataccatcac ggcgcatcac ctcagcaaca acacgcgcat aattttcggc
     3361 tgtcacgctg tccggctgcg tggcctgttc gtcagcctgc tggctgattc cggcaacgcg
     3421 gcggattaat cccgttattt cggcttctgt catgctgctg acctcattac accccgctaa
     3481 attcttccag tttctggcgg tggctgtcgc ttatatcaaa agcaaaatcc tcatgctctg
     3541 cctggaatgt accaaacgcc atcagcgccg ccacgctcgg gtctatcttg ttcggtgatt
     3601 ttttcttgtt cggcttgata ttggcgttcg cgtcactctg catcacgacg ttactcatcg
     3661 accaggacaa caccggatcg ccacgatgca caatcaccct gcggttaaca aaaacttcga
     3721 acgatttcgc cgccggactg aatctgaggt aggtttgcgg gaacggctcc acctcaaaac
     3781 ctgccccctg taattgcgtt ctgagatgcg tggcgttcca cgtatcaaag cccaccagcc
     3841 tgatattaaa tttctccgcg tcctgcatga tgtcatctct gatccggtcg taatcaatgc
     3901 agtcgccagg cgttgtgcgt atccagcctg ctttagccca ctgacgatag acagcgcggt
     3961 ttttattggc ggggttctgt agctggaact ccggcagata atgacgggaa accagcataa
     4021 tggttttacc taccggaaag gcatagcaca cgctggaaat atcgcttgtg gatgataaat
     4081 ccagccccgc gtaacactcc tggccgtgta aatcttcctc cgtgaacgtt ccggcacact
     4141 cagcccatgc gccgttaccc atccacgggg tagtcccctg acaccagata ttaaatcgct
     4201 ttgtcatcat ctccacccac tgcgacggaa taccccgcgc tttctggatg gttgaggcca
     4261 gtttttcacg atccacggaa acatcaatat tagggttcgc ttttatccac atcgccggat
     4321 catcaacctc gttttcgtcg tccagttcgt aaatcagtac aaaaattgaa tcgttgacct
     4381 cttcgccgtc caggatctgg cagcaataat catagtgctg cttacaggct gaaacgacgt
     4441 tactgcctga tgtggtaatg gcaaataaca gcccttcggg acgtgcgccc atccccagct
     4501 caagcgcaga ataaacgccg ttatcggggt gtaggtggta ttcgtccacg atggaaaggc
     4561 tggggtttgt tccctcgatg gttgccgctt ttgctgccag cggctttaac aggctgttgc
     4621 ttttcgggtg tatcacctta tgcgcctgaa tattcacccg cctgcgtaac ggtcgggata
     4681 aaaggcacat ctgacgcgca tcatcaaaga cgatccgcgc ctggtcacgg ctcaccgctg
     4741 cggtgtaaat atcctgctga ccgttctcca taatcagaaa ccagttagcc agaatcgcgg
     4801 cggtcgttga tttcgcattt ttgcgcggca cttcgataaa ggcgctcgtg tatttgcgcc
     4861 gtccggtggc cttaacctta aagccgagga tgcacgcaaa ggcgaactgc tgccacggct
     4921 ccagctcaat gggtctgcca cgcatcggtc cttttacgtg cgggcacacc ctggaaaagg
     4981 caataaaccg ctccacaacc tcacgatcga acgtgtaaag ggggctttta agatccgaaa
     5041 agtaccgttt aacggcctgt ttcagccgtt tacaggccgg aattttgccc gtttttacgt
     5101 cttctgcgta cttattccag gcggtcaagc tcgtcctctt cttctgtttc cggtggattt
     5161 ttacggcggc ttatcgggtc aaaaccaagc aaggaggcca tttttatcat cactctttca
     5221 gcgtcggatt tcgcgcttaa tgcggggttt ctgctctcgc cgccctgact gttaacaatg
     5281 ctgaacccgc gcgccgcaag gtctgcgacg gctttgcggt atatcgagta attgacgcaa
     5341 tacagttcca gattgctcca gtcggcgggg gtcaggtctc cccgttccgc aagctgccgc
     5401 gatttttccc gccactgctt cacggcgatg tcatccaggt aggcgggggc tttcggtggt
     5461 cttgccatgc ttatttttcg tcagattatt tttcaaaaaa ttcccgtgca taaaaatttg
     5521 gggaggcgtt cggtgcccgg cggggtcggg tttgtcctga aaacgccccc caccccgtca
     5581 tacagcctca tcagcgattg cggaaacatt ccataacctc gcggtcacgg tcggttaatc
     5641 gcttcgctgt gatgcgttct gcgcgtcctg acgctttatc tttatgccct gtttcctgtg
     5701 tcttccatgc gtcacgctgc cttataagtc cacggataag gcgtttttgt tcccgctcat
     5761 tcatcagcgt catacatcca gttattgcgg ttagcggccc gttcttcctc ttcacgaaat
     5821 ccacctgcgg cacgcttgct ttttgttgct ggatcaagcc acttcgtttt ctggttatga
     5881 cacgcctggc acaatggctg atggttccat tcgggccaga agagaacatc atcaccgcca
     5941 ttaatcggga taatgtgatc caccaccacg gcgggcgtat atatcccctt ctcaaggcat
     6001 cgcacgcata acgggttttt actcagatac atggcgcggt atttttccca ctgtctggag
     6061 tacccacgcg cgcggcggtg tcctcgtctg gcatcctctg cacgccatgc cgcccgcctg
     6121 tgctcctcac acttgccgga cttaacgcgc ctgttacagc ctggttctgt acatcgtctt
     6181 aatggttgcc acggcatcag tacaccccca catcacggta agccgtccag agcgcgccaa
     6241 tcgtcatggg tacgcgcgtt tttgcgttat ccgctaccag ctggcgattc tcgtacaggt
     6301 gagcgataaa catcatgcag ccaatcttta tggctggcgt gaactccagc ccgtcatcaa
     6361 agcgcctgcc tatatgtatc tggcacgcct caagcgatgc ggcaatgtat ccgctgatta
     6421 actggtcttc ctcgtcgcca tcgatgcggc aatggagttt cacttcttcc agggtgataa
     6481 gttcttctgt catttttccg cgccctcacg acaaagaatt tcaaggcgtg tcctggcggc
     6541 atccggcagc ggttgcccga tgatattcag tacacgcccc gccagcggcc ccgtattgac
     6601 ctttatccgg ctggtggcgt tgatgtcctt ccggtaccgt atccagatcc ttacggtacc
     6661 aatcgccagc tctgcacccg atgaaatggc ctccttgctg ctgatcatgt tcacgcttgc
     6721 ccagagtgtg tgaccgtcct cccacgtttc gagtatttcg ccggttatat ccctggtctg
     6781 tttcagggtc tgaatcgtta ccctgtcacg cagtcgccct atgttcattc cggtttttct
     6841 ccctcgctga tttttacttc ctgtttccat gcctggctga actcatcacc accatcacgc
     6901 ggggacagcc cttcacgttc gcgggcttcg ttcgggcaca taacgccgga tttgatgccg
     6961 cgttcatagg tggcaaagcg ttcgccaggt gtggcccgta acaggtccgc gctgtcaaac
     7021 tccacctgat accggatacc aggtacaggc gaggccacca gcagggcgtt ttttatctgc
     7081 tgctcaaagt tcgccagcca cgggcgcatt gtcatggtga gaaatgcgcg gcttgcctcg
     7141 ctgaaattgc tgtaggtgct gttgctgtat tcctgcagaa aaatcggcga gacgttgaac
     7201 attcgggcga tgtcttcaat ggtgaagcga cgggaggcca gccattcagc atcctgatta
     7261 ctcatgccca gctgctgata gctcataccc ccttcaagaa tgggcgtttt tccggcgttt
     7321 ctggctcctt tgtagcgttc cagtgcggct aatgcctgtt tgcccttcac gccgtccagc
     7381 cattcgcctg acgtgataac ccctgccgcc atcatgccat cacgcatcac gctcgcgccg
     7441 tggcgttgtt gggccagccc aagccccagc gattcgcggc agatggttac aggtgagcgc
     7501 cccataaaac cgtcatccgt ggagtaacga aggtggagaa cctcccacgg taaatagttg
     7561 cgggtgtttc cggtgtaggc gtcagtgatg cagtagcgcc agttatgctc acctgtctgc
     7621 tcaacgttca ccgactgcgg cgggtaagga tgtaaagcca ccggaaaacc atcacgcccc
     7681 cactgaatca ccgcataagc attaccgttt aacaggcagt ggcggatcat cattcgctta
     7741 aactggtagg gagtctgcca cgcgttcggg cgctcgttga ggatgtgatc gaccggatga
     7801 gaatcaagcc actcgcgggc ctccttcccc ttctcattgc gtaccaggta caggtaacac
     7861 ggcatggtag ccaccgcctc agagatgacc gtcacggcgt tcatgacggc gggcagtgat
     7921 tccgctgtcc ccgatgatac gtactcaccc gcccccgtgt tcgatgtgcc agccagcgcc
     7981 ataaattcat caagcgtcat gctgcgctgc tctttttttc ttctgaaaag ccacatatca
     8041 cacccccgct aaatccaccc accagcggcg attatccgca cgcggcattt tttctgggtg
     8101 ctgctcatac agggaacggc gggccagctc cacgccggaa tcggggtaag ccggtaccga
     8161 tgtaacggta atttcgtaca gttccgccac cagcacggtg cgcacgcatg gatctgttgt
     8221 ggtatcccat acatccttac gggagcgaaa gccaaagctc atccccgaga tgtccccacg
     8281 cttaaccagt tcgataacgt cgcgccctgt actggtatcc ggtggggtca gttcaaagcg
     8341 taaccctgtt tcgtcctctt ccagtttcag cgtgccggaa cgggtacgcc ccagcaacat
     8401 gctgtggtca tgctcataca ggccgcgaac gtcattaccc accgcaagcc attcagtaaa
     8461 cgctccccgc tggaattttt cgtaaaactc cccccatagc agctctgaaa gcttatccca
     8521 gcgaacaacg tagcctgtaa gcgtgttgct ggcgctggtg gtgatttccg atgaccggat
     8581 ttccatactc ttcataattt tttcacccat aaaacactaa aggggctttt aagccccttc
     8641 tgtatgctgt taatcgtcgt cctgtggcag ttccagaatc ttgatcgcgt tcgaatccac
     8701 cacgccaccg cctaaatatt tctgcgtgaa aattttgatg aagcccggct ctgtgaggtt
     8761 gtccggtctg gtgcgaacac ctgtttcgtg atcaacaatg tagtaaccgc gcttgaagtc
     8821 acccagggca ataacgttat caggcataaa ctccagatat tcgaccggaa ggcccagcaa
     8881 cgtatcagga tcacccgcct gtaaacggtc gcgccagatg taatcaccgt tcgcgttctt
     8941 caccttctga agttttgccg ccgtcgtgga gttaaccacc cagaccgcgt ttttgcggta
     9001 ttttttacgt aatgcaaatt tcaggtcgat cagcgggtcc gcagatgtcc acgccagaga
     9061 ttcggaaggt ttaattacct gcaacgtacc aaaatcacgc tctttgtcgt tcttctctgc
     9121 acggggtacg gataaaaaac cttttgcttt tttgtcaccg tcgcccacaa ccagatcgct
     9181 ttcttcggtt tccgtgaagg tgtcgccaat ctcacccgtc agccatgaaa ggatgtccac
     9241 atcggaaaaa tccacgattt cctgtgtggt gcgcgggtac gcatagaccg gatacagctt
     9301 aatgctcacc tcgttaatct gcggggtgct ggtctgttcg cgtgccttac cctcttcacc
     9361 gtggttaacg gtcacaccgc cagcggaaac aagctgctta aactcgttgc tgctgatttt
     9421 tttcacggta cagatgcggc gcatggtgga ctcatccgcc agcattcgca tgatttcggt
     9481 gttcagttcg gggataacgg tataaccacc atcagcggga acgcctgtac ttaatgcgcg
     9541 ggtttcacct gtcagaatgt agctgcgtag ttctgcgggg tcagtggtct ggctgctttt
     9601 acctggcttg ctgcgctctt cgtctgcaat ggcttcaagg cgggaaatgt ctttatcgag
     9661 ggattcagct ttagcgcgta attcgtcaaa ttttgcgccc tcagcatcgt taagactgcg
     9721 gttttctttt tcagcgttct ccagcatgtc gcgcatctga tttttaatgg cggttttctg
     9781 ctggcgtaat tcgattattc tcggcataaa aaaaagtcct ggggttaagt aaggaactcc
     9841 aggacgcggc aaacactcaa ccgtttttca taaggaaatc agcaatcgca ccgatcgttt
     9901 tcccgcctgg taatgaatat tggcgagcac attaacaggc gggaaagtgg ccccagcgtc
     9961 ctggcaccac gggcgagaat aatcatgatt cagttcgggt aaaatatgcc gatccgctca
    10021 gtgaacaacg tggaacaacc acgaacaaat aatttacaaa aaataacaaa aagccggatt
    10081 gctccggctg tatgtgctgt atgggtgctc agtctctgat ccctttcaga aattctatca
    10141 atgcatctat ctgttcagga tttaccgcca gcatttcacc ggacagagca catctcacaa
    10201 aaccatgctg atccttttca atcagtgcgc ccgtctccag gaatgcgcgg taatcattga
    10261 tgctcatcgt ctccatgttg tcagcatggt atttatcacg ctgttttaat atctcatcaa
//
LOCUS       DAJVUJ010000151        10278 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.151, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000151 DAJVUJ010000000
VERSION     DAJVUJ010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10278
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.151"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            177..2056
                     /gene="kup"
                     /locus_tag="OQQ48_003406"
                     /pseudo
     CDS             177..2056
                     /gene="kup"
                     /locus_tag="OQQ48_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
     gene            2223..2642
                     /gene="rbsD"
                     /locus_tag="OQQ48_003407"
     CDS             2223..2642
                     /gene="rbsD"
                     /locus_tag="OQQ48_003407"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCS1954056.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2650..4119
                     /gene="rbsA"
                     /locus_tag="OQQ48_003408"
                     /pseudo
     CDS             2650..4119
                     /gene="rbsA"
                     /locus_tag="OQQ48_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
     gene            4124..5089
                     /gene="rbsC"
                     /locus_tag="OQQ48_003409"
     CDS             4124..5089
                     /gene="rbsC"
                     /locus_tag="OQQ48_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HCS1954057.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGTIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            5114..6000
                     /gene="rbsB"
                     /locus_tag="OQQ48_003410"
                     /pseudo
     CDS             5114..6000
                     /gene="rbsB"
                     /locus_tag="OQQ48_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            6126..7055
                     /gene="rbsK"
                     /locus_tag="OQQ48_003411"
     CDS             6126..7055
                     /gene="rbsK"
                     /locus_tag="OQQ48_003411"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCS1954058.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            7059..8051
                     /gene="rbsR"
                     /locus_tag="OQQ48_003412"
     CDS             7059..8051
                     /gene="rbsR"
                     /locus_tag="OQQ48_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HCS1954059.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(8017..9444)
                     /gene="mdtD"
                     /locus_tag="OQQ48_003413"
     CDS             complement(8017..9444)
                     /gene="mdtD"
                     /locus_tag="OQQ48_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HCS1954060.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMFIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     RATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARHTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGSVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(9467..10159)
                     /locus_tag="OQQ48_003414"
     CDS             complement(9467..10159)
                     /locus_tag="OQQ48_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HCS1954061.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKKNFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KTCQALLRSPDK"
BASE COUNT         2504 a         2474 c         2718 g         2582 t
ORIGIN      
        1 tcggtcaact gatccttaac tgatcggcat tactcaaagg ggggtatttt tctttgagcc
       61 aggttaatgt ggccgcattt aggagtacga ttttgccgtt aatcgtgcat actgtgcgct
      121 tttttgtggg ccaagggact aagcacacat ttcatatttc aacgaaagac tagtctatga
      181 gcactgataa taagcaatca ttgcccgcga ttaccctcgc ggcgattgga gttgtctacg
      241 gcgatattgg taccagcccg ttatatacac ttcgtgaatg tttgtccggc cagtttggtt
      301 ttggcgttga acgcgatgcc gtgtttggct ttttatcgct gatcttctgg ctgctaatct
      361 ttgtggtttc cattaaatat ctcaccttcg tgatgcgggc aggatgcggg cagataacgc
      421 cggtgagggg gggatcctga cgttgatgtc gcttgccggg cgtaatacgt cggcgcgaac
      481 cacatcaatg ctggtgatta tggggctaat cggcggcagc tttttctatg gtgaagtagt
      541 cataacaccc gctatttcgg tgatgtcagc cattgaaggt ctggaaatcg tcgccccgca
      601 gctggatacc tggatagttc ccctctcaat tatcgttctc actttattat ttatgattca
      661 aaaacatggc acggctatgg tcggtaagct gtttgcgccg atcatgctga cctggttttt
      721 gattctggca ggactggggt tacgtagcat tattgctaac ccggaagtgc tgcatgcgct
      781 gaatccaatg tgggcggtgc atttcttcct tgaatacaaa acggtttctt ttattgcatt
      841 aggggcagtg gtgttgtcga ttacgggggt cgaggcgctg tatgctgata tggggcactt
      901 tggtaagttc cctattcgcc tggcgtggtt caccgtcgta ttaccttcct tgacccttaa
      961 ttacttcggt cagggagcgc tgttgttaaa gaacccggaa gcgattaaga acccgttctt
     1021 cctgttggca ccggactggg cgctgatccc gctgctgatc atcgccgcac tggcgacggt
     1081 aattgcctcg caggcggtta tctctggcgt cttctcattg acgcgtcagg cggtacgtct
     1141 gggatatttg tcgccgatgc gcattattca cacctccgaa atggagtcag ggcaaatcta
     1201 tattccgttt gtgaactgga tgctctatgt cgcggtcgtg attgtgattg tcagctttga
     1261 gcactccagc aacctggcgg cggcgtacgg gattgcggtg accggaacca tggtgctgac
     1321 gtctattctc tcgactaccg tggcacgtca gaactggcac tggaataagt attttgttgc
     1381 gctgatcctg attgctttcc tttgtgtcga tattccattg ttcaccgcta acctcgataa
     1441 actgctctcc ggcggttggt tgccattgag cctcggtacg gtgatgttta tcgtgatgac
     1501 cacctggaag agcgagcgtt tccgcttgct gcggcggatg catgaacatg gtaactctct
     1561 ggaagcgatg attgcttcgc tggagaaatc accgcctgtt cgcgtgcccg ggaccgcggt
     1621 gtatatgtcg cgtgcaatca acgtcattcc ctttgcgctg atgcataacc ttaaacataa
     1681 caaggtattg catgagcggg tgattctgtt aactctgcgt accgaagacg cgccatatgt
     1741 ccataatgtc cgtcgggtac agattgaaca actgtcgctc actttctggc gcgtggtggc
     1801 aagttatggt tggcgagaaa cgccaaacgt ggaagaagtt ttccaccgct gcggtctgga
     1861 aggattaagt tgccggatga tggaaacctc cttctttatg tcgcatgagt cgttgatcct
     1921 cggcaaacgc ccgtggtatt tgcgtctgcg cggcaagctg tacttgctgc tgcaacgtaa
     1981 tgcgctgcgt gcaccagatc aatttgaaat cccgccaaac agggttatcg aactgggtac
     2041 tcaggtcgaa atctaacgcc agacgcctcc tttctccata agggggcgtt tttgttttca
     2101 tggttaatca ccatgtaaaa cgtttcgagg ttgatcacat ttccgtaacg tcacgatggt
     2161 tttcccaact cagtcaggat taaactgcgg gtcagcgaaa cgtttcgctg atggagaaaa
     2221 aaatgaaaaa aggcaccgtt cttaattctg atatttcatc ggtgatctcc cgtctgggac
     2281 ataccgatac gctggtggtg tgtgatgctg gtttacccat ccccaaaagt acaacgcgta
     2341 tcgatatggc attaacccag ggtgtacctt cttttatgca ggtgctgggc gtcgttacaa
     2401 atgaaatgca ggtcgaggct gccattatcg cggaagagat caaacaacat aatccgcaac
     2461 tccacgaaac gttgctcact caccttgagc agctgcaaaa acaccaggga aataccattg
     2521 aaattcgtta caccacgcat gaacaattca aacaacaaac cgcagaaagt caggcggtaa
     2581 ttcgcagcgg agaatgttct ccgtatgcga atatcattct ctgtgctggc gtgacgttct
     2641 gaggccgtca tggaagcatt acttcagctt aaaggcatcg ataaagcctt cccgggcgta
     2701 aaagccctct cgggcgctgc gttaaatgtc tatccgggcc gcgtgatggc gctggtgggc
     2761 gaaaacggcg cgggtaaatc caccatgatg aaagtgctta ctggtatcta tgctcgcgat
     2821 gccggcacgc ttttatggct ggggaaagaa acgacattta ccgggccgaa atcttcccag
     2881 gaagccggga ttgggattat ccatcaggaa ctgaacctga tcccgcagtt gaccattgcc
     2941 gaaaacattt tcctcggtcg tgagtttgtt aatcgctttg gcaaaattga ctggaaaacc
     3001 atgtatgccg aagcggataa attgctggct aaacttaacc tgcgctttaa aagcgacaag
     3061 ctggtgggcg atctttccat cggtgaccag caaatggttg aaatcgccaa agtgctgagc
     3121 tttgagtcga aagtcatcat tatggatgaa ccgaccgatg cgctgaccga taccgaaacc
     3181 gaatccctgt tccgcgtcat ccgcgaacta aaatcgcaag gccgcggtat tgtctatatc
     3241 tcccaccgca tgaaagaaat cttcgagatt tgcgatgccg ttaccgtttt tcgtgacggg
     3301 caatttattg ctgagcgcga agtggcatca ctgaccgaag attcgctgat tgagatgatg
     3361 gtgggtcgca agctggaaga tcaatatcca cacctggaca aagcgccggg agatatccgc
     3421 ctgaaagtcg ataatctctg cggacctggc gttaacgatg tctcttttac tttacgcaaa
     3481 ggcgaaattc ttggcgtctc tggtttgatg ggcgcaggtc gtaccgaact gatgaaagtg
     3541 ctctacggcg cactaccgcg caccagcggt tacgtcaccc tggatgggca tgaagtcgtt
     3601 acccgttcac cgcaggatgg cctggcaaac ggcattgtgt atatctccga agaccgtaaa
     3661 cgtgacggtt tagtgttggg catgtcagta aaagagaaca tgtcgctgac agcgctgcgc
     3721 tacttcagcc gcgctggcgg cagtttgaag catgccgatg aacagtaggc tgtgagtgat
     3781 ttcattcgtc tgtttaatgt gaaaacaccg tcgatggaac aggcaattgg tctgctttcc
     3841 ggtggcaatc agcaaaaagt gttgatcctt gatgaaccta cccgtggcgt agatgtcggc
     3901 gcgaaaaaag agatctatca actgattaac cagttcaaag ccgatggctt gagcatcatt
     3961 ctggtgtcat cggagatgcc agaagtatta ggcatgagcg atcgcatcat cgtcatgcat
     4021 gaagggcatc tcagcgggga atttactcgt gagcaggcca cccaggaagt gttaatggct
     4081 gccgctgtgg gcaagcttaa tcgcgtgaat caggagtaaa aaaatgacaa cccagactgt
     4141 ctctggtcgc cgttatttca cgaaagcgtg gctgatggag cagaaatcgc ttatcgctct
     4201 gctggtgctg atcgcgattg tctcgacgtt aagcccgaac tttttcacca tcaataactt
     4261 attcaatatt ctccagcaaa cctcggtgaa cgccattatg gcggtcggga tgacgctggt
     4321 gatcctgact tcgggcatcg acttgtctgt aggttctctg ttggcgctaa ccggcgcagt
     4381 tgctgcatct atcgtcggca ttgaagtcaa tgcgctggtg gctgtcgctg ctgctctcgc
     4441 gttaggtgcc gcaattggtg cggtaaccgg ggtgattgta gcgaaaggtc gcgttcaggc
     4501 gtttatcgct acgctggtta tgatgctttt actgcgcggc gtgaccatgg tttataccaa
     4561 cggtagccca gtgaataccg gctttactga gaacgccgat ctgtttggct ggtttggtat
     4621 tggtcgtccg ctgggcgtac cgacgccagt ctggatcatg gggattgtct tcctcgcggc
     4681 ctggtacatg ctgcatcaca cgcgtctggg gcgttacatc tacgcgctgg gcggcaacga
     4741 agcggcaacg cgtctttctg gtatcaacgt caataaaatc aaaatcatcg tctattctct
     4801 ttgtggtctg ctggcatcgc tggccgggac cattgaagtg gcgcgtctct cctccgcaca
     4861 acccacggcg gggactggct atgagctgga tgctattgct gcggtggttc tgggcggtac
     4921 gagtctggcg ggcggaaaag gtcgcattgt tgggacgttg atcggcgcat taattcttgg
     4981 cttccttaat aatggattga atttgttagg tgtttcctcc tattaccaga tgatcgtcaa
     5041 agcggtggtg attttgctgg cggtgctggt agacaacaaa aagcagtaat aacgactaca
     5101 ggacatcttg aatatgaaca tgaaaaaact ggctaccctg gtttccgctg ttgcgctaag
     5161 cgccaccgtc agtgcgaatg cgatggcaaa agacaccatc gcgctggtgg tctccacgct
     5221 taacaacccg ttctttgtat cgctgaaaga tggcgcgcag aaagaggcgg ataagcttgg
     5281 ctataacctg gtggtgctgg actcccagaa caacccggcg aaagagctgg cgaacgtgca
     5341 ggacttaacc gttcgcggca ccaaaattct gctgattaac ccgaccgact ccgacgcagt
     5401 aggtaatgct gtgaagatgg ctaaccaggc gaacatcccg gttatcactc ttgaccgcca
     5461 ggcaacgaaa ggtgaagtgg tgagccacat tgcttctgat aacgtactgg gtggcaaaat
     5521 cgccggtgat tacatcgcga agaaagcggg tgaaggtgcc aaagttatcg agctgcaagg
     5581 cattgctggt acatccgcag cccgtgaacg tggcgaaggc ttccagcagg ccgttgctgc
     5641 tcacaagttt aatgttcttg ccagccagca gattttgatc gcactaaagg tttgaacgta
     5701 atgcagaacc tgttgaccgc tcatccggat gttcaggctg tattcgcgca gaatgatgaa
     5761 atggcgctgg gcgcgctgcg cgcactgcaa actgccggta aatcggatgt gatggtcgtc
     5821 ggatttgacg gtacaccgga tggcgaaaaa gcggtgaatg atggcaaact agcagcgact
     5881 atcgctcagc tacccgatca gattggcgcg aaaggcgtcg aaaccgcaga taaagtgctg
     5941 aaaggcgaga aagttcaggc taagtatccg gttgatctga aactggttgt taagcagtag
     6001 ttttaatcag gttgtatgac ctgatggtga cataaatacg tcatcgacag atgaacgtgt
     6061 aatataaaga aaagcagggc acgcgccacc ctaacacggt ggcgcatttt atggacatcc
     6121 cgaatatgca aaacgcaggc agcctcgttg ttcttggcag cattaatgct gaccacattc
     6181 ttaatcttca atcttttcct actccaggcg aaaccgtaac cggtaaccac tatcaggttg
     6241 catttggcgg caaaggcgcg aatcaggctg tggctgctgg gcgtagcggt gcgaatatcg
     6301 cgtttattgc ctgtacgggt gatgacagca ttggtgagag cgttcgccag cagctcgcca
     6361 ctgataacat tgatattact ccggtcagcg tgatcaaagg cgaatcaaca ggtgtggcgc
     6421 tgatttttgt taatggcgaa ggtgagaatg tcatcggtat tcatgccggc gctaatgctg
     6481 ccctttcccc ggcgctggtg gaagcgcaac gtgagcgtat tgccaacgcg tcagcattat
     6541 taatgcagct ggaatcacca ctcgaaagtg tgatggcagc ggcgaaaatc gcccatcaaa
     6601 ataagactat cgttgcgctt aacccggctc cggctcgcga acttcctgac gaactgctgg
     6661 cgctggtgga cattattacg ccaaacgaaa cggaagcaga aaagctcacc ggtattcgtg
     6721 ttgaaaatga tgaagatgca gcgaaggcgg cgcaggtact gcatgaaaaa ggtatccgta
     6781 ctgtactgat tactttagga agtcgtggtg tatgggctag cgtgaatggt gaaggtcagc
     6841 gcgttcctgg attccgggtg caggctgtcg ataccattgc tgccggagat acctttaacg
     6901 gcgcgttaat cacggcgttg ctggaagaaa aaccattgcc agaggcgatt cggtttgccc
     6961 atgctgcagc tgcgattgcc gtaacacgta aaggcgcaca accttccgta ccgtggcgtg
     7021 aagagatcga cgcattttta gacaggcaga ggtgacgctt ggctacaatg aaagatgttg
     7081 cccgcctggc gggcgtttct acctcaacag tttctcacgt tatcaataaa gatcgcttcg
     7141 tcagtgaagc gattaccgcc aaagttgaag cggcgattaa agaactcaat tacgcgccat
     7201 cagccctggc gcgtagcctc aaactcaatc aaacacatac cattggcatg ttgatcactg
     7261 ccagtaccaa tcctttctat tcagaactgg tgcgtggcgt tgaacgcagc tgcttcgaac
     7321 gcggttatag tctcgtcctt tgcaataccg aaggcgatga acagcggatg aatcgcaatc
     7381 tggaaacgct gatgcaaaaa cgcgttgatg gcttgctgtt actgtgcacc gaaacgcatc
     7441 aaccttcgcg tgaaatcatg caacgttatc cgacagtgcc tactgtgatg atggactggg
     7501 ctccgttcga tggcgacagc gatcttattc aggataactc gttgctgggc ggagacttag
     7561 caacgcaata tctgatcgat aaaggtcata cccgtatcgc ctgtattacc ggtccgctgg
     7621 ataaaactcc ggcgcgcctg cggttggaag gttatcgggc ggcgatgaaa cgtgcgggtc
     7681 tcaacattcc tgatggctat gaagtcactg gtgattttga atttaacggc gggtttgacg
     7741 ccatgcgcca actgttatca catccgctgc gtcctcaggc cgtctttacc ggaaatgacg
     7801 ctatggctgt tggcgtttac caggcgttat atcaggcaga gttacaggtt ccgcaggata
     7861 tcgcggtgat tggctatgac gatatcgaac tggcaagctt tatgacgcca ccattaacca
     7921 ctatccacca accgaaagat gaactggggg agctggcgat tgatgtactc atccatcgga
     7981 taacccagcc gacccttcag caacaacgat tacaacttac tccgattctg atggaacgcg
     8041 gttcggctta gatttacgct gtcttttgat caaattatta ccatcggttg ttttcagaag
     8101 catgaacatt gctgctgaag caacagtaat aatgcccatc gtgataaacg tatagtggaa
     8161 ttgttcgaca gtcgttgtgc cttccatccc ttcataaacg cgaaggacgg ccgcacttac
     8221 agcaacgcct aaactaatcg acagttgctg cgtgaccgcc agaacactgt taccgctgct
     8281 ggcgttgtca tcggtcagat cggcaagtgt gatggtattc atcgcggtaa attgcgtcga
     8341 catagccatc cctaatataa acaacggcaa gatcagcatc catatcgcca ttgccggtga
     8401 ttgcaaagag aactgagcga tcattagccc aataatcacc gtgatcccca ctaacgtatg
     8461 gcgatagccc agacgacgta agacttgggt aaccatcgat tttgcaataa tggaacctaa
     8521 cgctgtcggt gccatcatac agccggcaat aaacgcctga taaccaaatc ctacctgtaa
     8581 catcaatggc ataaggaacg gtacactacc ggtccccaga cgggttgcaa tattgcctac
     8641 gataccgatc gagaaagtgc gggttttaaa taaatctaat gaaattaatg ggtttggcgt
     8701 gtgtcgcgca tggagaatat agagaagcag taacccgatg ctggtgacaa ttaccgtcaa
     8761 ggcaatccag ctggcgacaa tcttttcccc gaatagctct attccgcttg agaagagaac
     8821 aaggctgagg ccaaacagca aaaagccagt gatatcgaat ctgcgtcgtg cggtggtgaa
     8881 attgggcata tgtttgcgcg cgtaaagaag gcccgcaata cctatgggga tattgattaa
     8941 aaatatccag tgccaggttg ccctggtgac cagcacgccg ccaagaacgg ggcctaaaat
     9001 tggccccacc agacccggca tggcgacaaa attcaataca ggaagaagtt cattacgagg
     9061 ataagcacgc agtaaggcca gccgagcaac aggcatcatc attgcgccgc ctatcccctg
     9121 aataacccgg aagacaacca gctgtggtag cgaattagaa agtgcgcagg ccagagaacc
     9181 caacgtgaac agactcacgg caagggtaaa aatgcgacgc gtaccgaagc gatcggctag
     9241 ccatccgctt accggaataa acatcgccac cgtcagcgta taactgatga tggctgattg
     9301 catcgcgaga ggagaacgat taaggctatg agcgattgcg ggtaaggcgg tattcagaat
     9361 agtggcatca agtgcctgca tgaagaaggc catcgccgcg atccacggca aacccgccat
     9421 actacgcttc tttttatcgc tcattcaatg tcctgttatc gggttatcac ttatcaggtg
     9481 agcgtagcag cgcctgacaa gttttaaatg ccgcgtcgcc atcgctttgg ataatcgcat
     9541 cgacaatcgc ctggtgcaga tccagcttta tcactgtgtc gctggtaatt gacgtgaagt
     9601 aagtgtgata aaccgaatgg aatagcgagg cgaatgaggt caaaaacgga ttggcgctca
     9661 tttcatagat atgctcatgc caggccatat cgacttcgat ccagcgttca cggcgaaagt
     9721 tcttttttaa tgccgccatt tcggccatta acgtattgag atgcgccttc tgttccgcgg
     9781 tgccaaccgt tgctgccagt aggcaggctt gcggctccag acagatacgc ataaccagaa
     9841 agtgatcgat gacctgatga aagttctctt ctgtcatcca ccaggtaagc aattcctgat
     9901 caagaaaatt ccagtttgat tgtggcatga cccgggtacc aattcgcggt cgcggtaaaa
     9961 ccatcccttt tgccgttaac gttttgaccg cttcgcgtac cgctgtacga ctcactccaa
    10021 attgctcgcc cagctcaatc tcaccaggca aaatggtgcc gggttcatat tcacctttta
    10081 agatgcgttg cgccagcttc tcagccagaa catacgaaag gtttttctgt gcagctaact
    10141 gttgtgcgct taaaggcatt actaatcttc ctttttcttt ttattcctcc ttagtatgcc
    10201 accaggaagt gtgattacgg ttgcaaaaac ggcaaattgc ttgttttatg gcacattaac
    10261 ggggcttttg ctgaaaaa
//
LOCUS       DAJVUJ010000152        10193 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.152, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000152 DAJVUJ010000000
VERSION     DAJVUJ010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10193
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.152"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(8..1905)
                     /gene="espL1"
                     /locus_tag="OQQ48_003415"
                     /pseudo
     CDS             complement(8..1905)
                     /gene="espL1"
                     /locus_tag="OQQ48_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            2427..4355
                     /gene="thrS"
                     /locus_tag="OQQ48_003416"
     CDS             2427..4355
                     /gene="thrS"
                     /locus_tag="OQQ48_003416"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCS1954062.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHAARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            4359..4901
                     /gene="infC"
                     /locus_tag="OQQ48_003417"
     CDS             4359..4901
                     /gene="infC"
                     /locus_tag="OQQ48_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCS1954063.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQRVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            4998..5195
                     /gene="rpmI"
                     /locus_tag="OQQ48_003418"
     CDS             4998..5195
                     /gene="rpmI"
                     /locus_tag="OQQ48_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCS1954064.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            5248..5604
                     /gene="rplT"
                     /locus_tag="OQQ48_003419"
     CDS             5248..5604
                     /gene="rplT"
                     /locus_tag="OQQ48_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCS1954065.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    5721..5845
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            5727..5771
                     /gene="pheM"
                     /locus_tag="OQQ48_003420"
     CDS             5727..5771
                     /gene="pheM"
                     /locus_tag="OQQ48_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HCS1954066.1"
                     /translation="MNAAIFRFFFYFST"
     gene            6054..7037
                     /gene="pheS"
                     /locus_tag="OQQ48_003421"
     CDS             6054..7037
                     /gene="pheS"
                     /locus_tag="OQQ48_003421"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCS1954067.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DTTRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            7052..9439
                     /gene="pheT"
                     /locus_tag="OQQ48_003422"
     CDS             7052..9439
                     /gene="pheT"
                     /locus_tag="OQQ48_003422"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCS1954068.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDCLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            9444..9743
                     /gene="ihfA"
                     /locus_tag="OQQ48_003423"
     CDS             9444..9743
                     /gene="ihfA"
                     /locus_tag="OQQ48_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HCS1954069.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            complement(10047..10187)
                     /locus_tag="OQQ48_003424"
     CDS             complement(10047..10187)
                     /locus_tag="OQQ48_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HCS1954070.1"
                     /translation="MSQKSLTAITFCVTAILIIWMLHGSLCEIRMSFWGAEFAAFLQC
                     KQ"
BASE COUNT         2694 a         2297 c         2605 g         2597 t
ORIGIN      
        1 tagttggtta taaatacgtc tttagttcat cagcactaaa actcaggctt tggctgatta
       61 catttatagc agcataatat gtttctacag ttttataatg attatgatga atggctatat
      121 gaacagctga catgttgtca tgatttttag cggccaacag tgtgaataac tgacgttgac
      181 taaaagaata tttttttgca aaaatattca gcgtcgatat ataagataac acgacgtctt
      241 cattaccctt gctcatggcg atgtataata caggggttcc atgtgctgta gcgcctttta
      301 ataaatccat gatgttagct ttgctcaact tgtatttaaa ggcgatgcca ttaattttag
      361 agaggaaccg tgtgacacac aaagggtgat tattttccat tgcagcaaac aaccctagtt
      421 caccatcacg tgttttcatt gctaaaatat ccatcactat ttttttgttt aaaagctctt
      481 gtgctattgg agtggttaat gcattcagaa agatatcgat tgtttcatcg aacccataca
      541 acatggagat atagagccca ggtgtttcgt tagcactttt agtttgcagt aatttaacca
      601 gattatcttc atgaatgata tgattgtata tcagtgactg aatttcttgc ataaatattt
      661 ttacgttgtc agtatgtccg ttcatcatag ccagaaataa agcaggtgta ccaacgctat
      721 tctgcgcagt tattatttca atgatttctt tttcagggag tatgggcaac tgtttgaata
      781 atgtaataag ctgttgagtc tggcctgtgg ccattattaa caacattgcc gatggatgta
      841 taggggcaaa tggcattcgt gatgcaatac ccgcgaaaat agcccctgga actcccacag
      901 gtaatagggg cagaatagca attgcacagt tgcgaacata cctatcagcc caggtctgag
      961 catcgggacc tgaactgatg aacttatcaa gcgaaagaaa gccccgtgct gtcctgtggg
     1021 tgcctctgac ggcaacgttg gtatcgttcg ggtcgtagac ggacactaca tagtttaatt
     1081 gtccatacac taactgcttt attcgtagcc ttactgacaa agtatgattg tctatatata
     1141 aaatcgcgga catatgactg acattgtttg ccatcattcg acaaaagaaa atatctaaag
     1201 cgaagccaaa atccttactc gcgataacgc gactccctgc aggaggatgg cgaagtaatg
     1261 ctctgaactc cgcatcccgg actaagggga catgctgctg tatggattca acggaggagt
     1321 aatgtttaat caggttgact ttgccttttc catttctcaa tgaatcaagg gcgtattgca
     1381 atgacagatg gcgacaaaaa atagcgtcag aaggagattg caaaaaacat gcactaccat
     1441 tgagatttac tgttggttgt atagtttttt ctctatggat ttttttagac agtttcatac
     1501 aatggttacg atcttcggca gttaactgtt cctgctcaat ataatccagt atgcttcgca
     1561 aatggttagc cttaataggc gtggtttcca gaaataaggg aatgatttct tttttatagt
     1621 gtaatgtaat aagtatattt tctgcatgga tgggtgaagt ggcaaagtta atgataaaaa
     1681 gggcagtgga gtaacaattt gtgatatttt tcgacagttt gcggtaaatt cctgagtaat
     1741 acagtatgac agcatcgaac ttaagtcaga ggtatcaagt agggtgatga gttggcgcgc
     1801 aaattgatta ataagctctg atgaaggcgt tggtatgtcg ctatcgatgt gcaatctatg
     1861 aggagactta taatgagttc tgataatgat atcgttttga ctcataatct attcacctta
     1921 tatatactgg aggttttgag gatgatgatt aattattatc gccagaacga aatgtgcacg
     1981 aaaaattgct ataagaattg tgtcactttt atcattgtga tatgaccact tcgtcattaa
     2041 tctcatgatc gtttaaatac taccgccaaa tcacgcaacg aactttgctg gattcagttt
     2101 atgttgctgt aaatccgctc gcagtaaacc tttcagacgc accgtgatgt tatcagtggt
     2161 tctttaagcg ttttgctggt gtactcacta caaacgaatt gcgaatcgaa tcaatgtgaa
     2221 acggaaaggt acaatctccc tttttttagt gttaacatcg ttcaaccggg tggaaggtga
     2281 gaggttaatg tctttcattt gtgtacctga ttgcgaacca atttagcatt tgttggctag
     2341 atggtttcgc aatgaactgt taataaacaa atttttcttt gtatgtgatc tttcgtgtgg
     2401 gtcaccactg caaataagga tataaaatgc ctgttataac tcttcctgat ggcagccaac
     2461 gccattacga ccacgctgta agccccatgg atgttgcgct ggacattggt ccaggtctgg
     2521 cgaaagcctg tatcgcaggg cgcgttaatg gcgaactggt tgatgcttgc gatctgattg
     2581 aaaacgacgc acaactgtcg atcattaccg ccaaagacga agaaggtctg gagatcattc
     2641 gtcactcctg tgcgcacctg ttagggcatg cgattaaaca actttggccg cataccaaaa
     2701 tggcaatcgg cccggttatt gacaacggtt tttattacga cgttgatctt gaccgcacgt
     2761 taacccagga agatgtcgaa gcactcgaga agcggatgca tgagcttgct gagaaaaact
     2821 acgacgtcat taagaagaaa gtcagctggc acgcagcgcg tgaaactttc gccaaccgtg
     2881 gggagagcta caaagtctcc attcttgacg aaaacatcgc ccatgatgac aagccaggtc
     2941 tgtacttcca tgaagaatat gtcgatatgt gccgcggtcc gcacgtaccg aacatgcgtt
     3001 tctgccatca tttcaaacta atgaaaacgg caggggctta ctggcgtggc gacagcaaca
     3061 acaaaatgtt gcaacgtatt tacggtacgg cgtgggcaga caaaaaagca cttaacgctt
     3121 acctgcagcg cctggaagaa gccgcgaaac gcgaccaccg taaaatcggt aaacagctcg
     3181 acctgtacca tatgcaggaa gaagcgccgg gtatggtatt ctggcacaac gacggctgga
     3241 ccatcttccg tgaactggaa gtgtttgttc gttctaaact gaaagagtac cagtatcagg
     3301 aagttaaagg tccgttcatg atggaccgtg tcctgtggga aaaaaccggt cactgggaca
     3361 actacaaaga tgcaatgttc accacatctt ctgagaaccg tgaatactgc attaagccga
     3421 tgaactgccc gggtcacgta caaattttca accaggggct gaagtcttat cgcgatctgc
     3481 cgctgcgtat ggccgagttt ggtagctgcc accgtaacga gccgtcaggt tcgctgcatg
     3541 gcctgatgcg cgtgcgtgga tttacccaag atgacgcgca tatcttctgt actgaagaac
     3601 aaattcgcga tgaagttaac ggatgtatcc gtttagtcta tgatatgtac agcacttttg
     3661 gcttcgagaa gatcgtcgtc aaactctcca ctcgtcctga aaaacgtatt ggcagcgacg
     3721 aaatgtggga tcgtgctgag gcggacctgg cggttgcgct ggaagaaaac aacatcccgt
     3781 ttgaatatca actgggtgaa ggcgctttct acggtccgaa aattgaattt accctgtatg
     3841 actgcctcga tcgtgcatgg cagtgcggta cagtacagct ggacttctcc ttgccgtctc
     3901 gtctgagcgc ctcctatgtg ggcgaagaca acgagcgtaa ggtaccggta atgattcacc
     3961 gcgcaattct ggggtcgatg gaacgtttca tcggtatcct gaccgaagaa ttcgctggtt
     4021 tcttcccgac ctggcttgcg ccagttcagg ttgttatcat gaatattacc gattcacagt
     4081 ctgaatacgt taacgaattg acgcaaaaac tatcaaatgc gggcattcgt gttaaagcag
     4141 acttgagaaa tgagaagatt ggctttaaaa tccgcgagca cactttgcgt cgcgtcccat
     4201 atatgctggt ctgtggtgat aaagaggtgg aatcaggcaa agttgccgtt cgcacccgcc
     4261 gtggtaaaga cctgggaagc atggacgtaa atgaagtgat cgagaagttg caacaagaga
     4321 ttcgcagccg cagtcttaaa caattggagg aataaggtat taaaggcgga aaacgagttc
     4381 aaacggcgcg ccctaaccgt atcaatggcg aaattcgcgc ccaggaagtt cgcttaacag
     4441 gtctggaagg cgagcagctt ggtattgtga gtctgagaga agctctggag aaagcagaag
     4501 aagccggagt agacttagtc gagatcagcc ctaacgccga gccgccggtt tgtcgtataa
     4561 tggattacgg caaattcctc tatgaaaaga gcaagtcttc taaggaacag aagaaaaagc
     4621 aaagagttat ccaggttaag gaaattaaat tccgtcctgg tacagatgaa ggcgactatc
     4681 aggtaaaact ccgcagcctg attcgctttc tcgaagaggg tgataaagcc aaaatcacgc
     4741 tgcgtttccg cggtcgtgag atggcgcacc agcaaatcgg tatggaagtg cttaatcgcg
     4801 tgaaagacga tttgcaagaa ctggcagtgg tcgaatcctt cccaacgaag atcgaaggcc
     4861 gccagatgat catggtgctc gctcctaaga agaaacagta aggccttcaa gtagcactgt
     4921 ctgtggagcc ttcgggcttc acaggctttg ttcgcctttg tttcgtttat taacaatgcg
     4981 aagtggaagt tattaaaatg ccaaaaatta agaccgtacg cggtgctgct aagcgcttca
     5041 aaaaaaccgg taaaggtggt tttaagcaca agcacgctaa cctgcgtcac attctgacca
     5101 aaaaagcgac caaacgtaaa cgtcacctgc gtccgaaagc catggtttcc aaaggcgatc
     5161 tgggcctggt aatcgcgtgc ctgccgtacg cataagccgt taacgttttt aactttttaa
     5221 ttagaatata gatacaggag agcacatatg gctcgcgtaa aacgtggtgt tattgcacgc
     5281 gcacgtcaca agaaaatttt gaaacaagct aaaggctact acggtgcgcg ttctcgcgta
     5341 taccgcgttg ccttccaggc tgttatcaaa gctggtcagt atgcttaccg tgaccgtcgt
     5401 caacgtaagc gtcagttccg tcaactgtgg attgcacgta tcaacgcagc agcacgtcag
     5461 aacggtattt cttacagcaa attcatcaat ggcctgaaaa aagcctctgt tgaaatcgac
     5521 cgtaagatcc tggctgatat cgcagtattc gacaaagtag cgttcaccgc tctggttgaa
     5581 aaagcgaaag cagctctggc ataagccagt tgaaagaggg agctagtctc cctcttttcg
     5641 tttcaacgcc atcaaaacat tgacttttat cgccgtagcc ttttcaataa aggtcttttg
     5701 aagagtacca aaaggtaacg caagcaatga atgctgctat tttccgcttc tttttttact
     5761 ttagcacctg aatccaggag gctagcgcgt gagaagagaa acggaaaaca gcgcctgaaa
     5821 gcctcccagt ggaggctttt tttgtatgcg cgttttgaaa aataaaaagc cagtctatca
     5881 ggcgtaattg tcgcagccag tctggatacg gccagcgcgg agaaaccgga gcgtatttaa
     5941 gtacgtgaga atttcgagca ctgcccggga ccaaaatggc aagtaaaata gcctgatggg
     6001 ataggctcta agtccaacga accagtgtca ccactgacac aatgaggaaa accatgtcac
     6061 atctcgcaga actggttgcc agtgcgaagg cggccattag ccaggcgtca gatgttgccg
     6121 cgttagataa tgtgcgcgtc gaatatttgg gtaaaaaagg gcacttaacc cttcagatga
     6181 cgaccctgcg tgagctgccg ccagaagagc gtccggcagc tggtgcggtt atcaacgaag
     6241 cgaaagagca ggttcagcag gcgctgaatg cgcgtaaagc ggaactggaa agcgcagcgc
     6301 tgaatgcgcg tctggcggcg gaaacgattg atgtctccct gccaggtcgt cgcattgaaa
     6361 acggcggtct gcatccggtt acccgtacca tcgaccgtat cgaaagtttc ttcggtgagc
     6421 ttggctttac cgtggcaacc gggccggaaa tcgaagacga ttatcataac ttcgatgctc
     6481 tgaacattcc tggtcaccac ccggcgcgcg ctgaccacga cactttctgg tttgacacta
     6541 cccgcctgct gcgtacccag acctctggcg tacagatccg caccatgaaa gcccagcagc
     6601 caccgattcg tatcatcgcg cctggccgcg tttatcgtaa cgactacgac cagactcaca
     6661 cgccgatgtt ccatcagatg gaaggtctga ttgttgatac caacatcagc tttaccaacc
     6721 tgaaaggcac tctgcacgat ttcctgcgta acttctttga ggaagatttg cagattcgct
     6781 tccgtccttc ctacttcccg ttcaccgagc cttctgcaga agttgacgtc atgggtaaaa
     6841 acggtaaatg gctggaagtg ctgggttgcg ggatggtgca tccgaacgtg ctgcgtaacg
     6901 ttggcatcga cccggaagtt tactctggtt tcgccttcgg gatggggatg gagcgtctga
     6961 ctatgttgcg ttacggcgtc accgacctgc gttcattctt cgaaaacgat ctgcgtttcc
     7021 tcaaacagtt taaataaggc aggaatagat tatgaaattc agtgaactgt ggttacgcga
     7081 atgggtgaac ccggcgattg atagcgatgc gctggcgaat caaatcacta tggcgggcct
     7141 ggaagttgac ggtgtagaac cggttgccgg tagcttccac ggcgtggtcg ttggtgaagt
     7201 ggttgagtgt gcgcagcatc cgaacgctga caaactgcgt gtgacaaaag tgaatgtcgg
     7261 cggcgatcgc ctgctggaca tcgtctgtgg tgcgccaaac tgccgtcagg gcctgcgtgt
     7321 ggcggtagct accattggtg ctgttctgcc gggtgatttc aaaattaaag cggcgaaact
     7381 gcgtggcgaa ccgtctgaag ggatgctgtg ctccttctct gaactgggaa tttctgacga
     7441 tcacagcggc attatcgaac tgcctgcaga tgcgccgatt ggcactgaca tccgcgaata
     7501 cctgaaactc gatgacaaca ccatcgaaat cagcgtaacg ccaaaccgtg ccgactgctt
     7561 aggtatcatt ggtgttgcgc gtgacgttgc cgtgctgaat cagctgccgc tggttgaacc
     7621 ggaaatcgtt ccggttggtg cgaccatcga cgacacgttg ccgattacag tcgaagcgcc
     7681 tgaagcctgc ccgcgctatc ttggccgtgt ggtaaaaggc attaacgtta aagcgccaac
     7741 tccgctgtgg atgaaagaaa aactgcgtcg ttgcgggatc cgttctatcg atgcagttgt
     7801 tgatgtcacc aactatgtac tgctcgaact gggccagccg atgcacgctt tcgataaaga
     7861 tcgcattgaa ggcggcattg tggtgcggat ggcaaaagag ggcgaaacgc tggtgctgct
     7921 tgatggcact gaagcgaagc tgaatgctga cactctggtc atcgccgacc acaacaaggc
     7981 gctggcgatg ggcggcatct ttggtggcga acactctggc gtgaatgacg aaacgcaaaa
     8041 cgtcttgctg gaatgcgctt tcttcagccc gctgtctatc accggtcgtg ctcgtcgtca
     8101 tggcctgcat actgatgcgt ctcaccgtta tgagcgtggc gttgatccgg cactgcagca
     8161 caaagcgatg gaacgtgcga cccgtctgct gatcgacatc tgcggtggtg aggctggtcc
     8221 ggtaattgat atcaccaacg aagcaacgct gccgaagcgt gcaaccatca ctttacgtcg
     8281 tagcaaactg gattgcctga tcggccatca tattgctgat gaacaggtaa ctgacattct
     8341 gcgtcgtctc ggctgcgaag tgaccgaagg caaagacgag tggcaggcag ttgcgccgag
     8401 ctggcgtttc gatatggaga ttgaagaaga tctggtcgaa gaagtcgcgc gtgtttacgg
     8461 ctacaacaac atcccggatg agccggtaca ggcaagcctg attatgggta ctcaccgtga
     8521 agctgacctg tcgctcaagc gcgtgaaaac gctgctcaac gacaaaggct atcaggaagt
     8581 gatcacctac agcttcgttg atccgaaagt gcagcagatg atccatccag gcgttgaagc
     8641 cttactgctg ccaagcccga tctctgttga aatgtcagca atgcgtcttt ctctgtggac
     8701 cggcctgctg gcaaccgtgg tgtacaacca gaaccgtcag cagaaccgtg tgcgcatttt
     8761 cgaaagcggt ctgcgtttcg taccagatac tcaggcaccg ttgggcattc gtcaggatct
     8821 gatgttagcc ggtgtgattt gcggtaaccg ttacgaagag cactggaacc tggcaaaaga
     8881 gaccgttgat ttctatgatt tgaaaggcga tcttgaatcc gttctcgacc tgaccggtaa
     8941 actgaatgag gttgagttcc gtgcagaagc gaatccggca ctgcatccgg ggcaatccgc
     9001 agcgatttat ctgaaagggg aacgtattgg ttttgttgga gttgttcatc ctgaactgga
     9061 acgtaaactg gatcttaacg gtcgcactct ggtgttcgaa ctggagtgga acaagctcgc
     9121 agaccgcgtg gtgcctcagg cgcgcgagat ttctcgcttc ccggcgaacc gtcgtgacat
     9181 cgcggtggtg gtcgcagaaa acgttcccgc agcggatatt ttatccgaat gtaagaaagt
     9241 tggcgtaaat caggtagttg gcgtaaactt atttgacgtg taccgcggta agggtgttgc
     9301 ggaggggtat aagagcctcg ccataagcct gatcctgcaa gataccagcc gtacactcga
     9361 agaagaggag attgccgcta ccgtcgccaa atgtgtagag gcattaaaag agcgattcca
     9421 ggcatcattg agggattgaa cctatggcgc ttacaaaagc tgaaatgtca gaatatctgt
     9481 ttgataagct tgggcttagc aagcgggatg ccaaagaact ggttgaactg tttttcgaag
     9541 agatccgtcg cgctctggaa aacggcgaac aggtgaaact ctctggtttt ggtaacttcg
     9601 atctgcgtga taagaatcaa cgcccgggac gtaacccgaa aacgggcgag gatattccca
     9661 ttacagcacg gcgcgtggtg accttcagac ccgggcagaa gttaaaaagc cgggtcgaaa
     9721 acgcttcgcc caaagacgag taatctgatc taactaaaaa ggccgctctg cggccttttt
     9781 tctttcaata actaattgat ttataatgtt ttatatctct tcgttccacg atgcgtccac
     9841 atttagtggg gggaggggag taagcccgct acattgtggg ctttgtctta tagtctgatc
     9901 actgtttcaa gtcggtgagg acaccaaaaa gaacccccgc agtttttacg ctgcgggttt
     9961 gttgttcatg tctgtgagat agggtgcctt atcgacctta ccctggcaac cgattgacgg
    10021 gggattgctc ccccgtcgcg gtttccttac tgcttacact gtaagaacgc cgcaaactcc
    10081 gctccccaga agctcatccg tatttcgcac agcgaaccgt gcagcatcca gatgatgagg
    10141 attgccgtca cgcagaacgt gatggccgta agcgattttt gcgacatagc gct
//
LOCUS       DAJVUJ010000153        10158 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.153, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000153 DAJVUJ010000000
VERSION     DAJVUJ010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10158
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.153"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(487..849)
                     /locus_tag="OQQ48_003425"
     CDS             complement(487..849)
                     /locus_tag="OQQ48_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HCS1954071.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
     gene            1134..1343
                     /locus_tag="OQQ48_003426"
     CDS             1134..1343
                     /locus_tag="OQQ48_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1954072.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            complement(1355..1942)
                     /gene="mtlR"
                     /locus_tag="OQQ48_003427"
     CDS             complement(1355..1942)
                     /gene="mtlR"
                     /locus_tag="OQQ48_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HCS1954073.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(1942..3090)
                     /gene="mtlD"
                     /locus_tag="OQQ48_003428"
     CDS             complement(1942..3090)
                     /gene="mtlD"
                     /locus_tag="OQQ48_003428"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCS1954074.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPSMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            complement(3320..5221)
                     /gene="mtlA"
                     /locus_tag="OQQ48_003429"
     CDS             complement(3320..5221)
                     /gene="mtlA"
                     /locus_tag="OQQ48_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HCS1954075.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLT
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSFDDSSANLFKLGAENIFLGRKAATKEE
                     AIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKTGVVF
                     CQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTTSVDE
                     VLELLAGRK"
     gene            5758..6120
                     /locus_tag="OQQ48_003430"
     CDS             5758..6120
                     /locus_tag="OQQ48_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCS1954076.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIASLSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            6123..7259
                     /locus_tag="OQQ48_003431"
     CDS             6123..7259
                     /locus_tag="OQQ48_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCS1954077.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGGLQSLTVVPGTDGVQGTIELDPND
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(7719..8156)
                     /locus_tag="OQQ48_003432"
     CDS             complement(7719..8156)
                     /locus_tag="OQQ48_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1954078.1"
                     /translation="MADKAILWALISASTKEERKACSLSYFACKAAEAELGLAYMAAN
                     DNKEFLTSLSNIMRYKIDAGLSESYTCYLLSKGKIIRPYLKNLNPLQLAADCIETVNK
                     IKDKNKKIIDINSVNICSDDKNIKLRVNSTIMAIDDSIKCIDE"
     gene            complement(8864..9325)
                     /locus_tag="OQQ48_003433"
     CDS             complement(8864..9325)
                     /locus_tag="OQQ48_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCS1954079.1"
                     /translation="MKACLLLFFYFSLIDQLHGADVKIKENESVMGSTAMTYDLSEEK
                     LMKLKYKSQHGDSEASFRLYQYYCFTKNNIDKQLRFLERSASQGNVTAQFNYGVFLSD
                     TNPTLSEYYNLNRAIYWMEFAVNNGNIDAKSKLQELKKLKRMDRRKNKENP"
     gene            complement(9337..>10158)
                     /locus_tag="OQQ48_003434"
     CDS             complement(9337..>10158)
                     /locus_tag="OQQ48_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCS1954080.1"
                     /translation="YTPARKIHLYHCDHRGLPLALISTEGATAWCAEYDEWSNLLSDE
                     NPHHLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGWNFYQYPLNP
                     VINVDPQGLVDINLYPESDLIHSVADEINIPGVFTIGGHGTPTSIESATRSIMTAKDL
                     AYLIKFDGNYKDGMTVWLFSCNTSKGQNSFASQLAKELHTNVIGPDTLWTWWGRGTNG
                     KLKMDTVLTAPTNLNSNKDLMAITTKDLGNWITYGPSGHPISNMQGTPEKPSDIR"
BASE COUNT         2605 a         2405 c         2314 g         2834 t
ORIGIN      
        1 attattggtg atttatgttg ctttacatta atgacagtga tgtgtgtcga ttaaatattg
       61 ttttaagcat tcatcataaa aatgtatcat tcgtttcaaa agatgtctgt gtttgtatga
      121 atatattatt ttcgtggatt ctaatgatga ttattaataa atgtcaaata tgaaagaaac
      181 catgtctggg aaaaaattta cagataattt attgtatgaa tatgatgatt aaaatttcac
      241 aattaaatcc gaaaaaaaga gacgtaactg cttgttaatt aataaatgat atcttctata
      301 tatttttatt gtttataggt ggctatttcg gtaaaaatgc attttcagaa tatcaaccag
      361 cggtaaatcg acgtaaaggg agtgaattgt ttggtaatta ggcgagtaga caggagagtc
      421 cccgcattga ccagctaagg caagccagat gcgggttgat atgatcgaac cagtcgtctg
      481 aggcgcttaa aacgcagtct tagagaatcc agtcatctcc tgcaggccca tttcacggcc
      541 cagcgcggtc attgggtgca caaccaccag tccgcgaaca cttttcttca gcttgcccat
      601 atcagcctgc tcttttttgg taattgcgcg ctgaaacggc atcttcatca gcttctgtgc
      661 ttctttactc agtttttggc tatgaacctc acgcaggcga gcaatttctg cttccagcgt
      721 ggctttctct ttttccagct cggcatattt gtctgcggct tcaaccagcg acagatcggc
      781 ctgctggtgg cggatggcgt ccaggcgatc gctgagacgt ttgatttcgt ttttttcgac
      841 ttctttcatg tgatggcttc tgataaagat aaatttatag cccctagtat gcgcgaccag
      901 gtgctttgtt cacaactgat gacattgtaa tagttctgca acgtaatcat taacatgctc
      961 aaaaactacg caattcgttg attaattaag aaatctatag aaaaaagata ataaacagca
     1021 gcggatgatt attgaggatt cagcacgttt tccttaatgt catctttacc gggaacctaa
     1081 actaaaattg gtggccgcgc attcccggcg cggggtaata cggagatatc atcatgggca
     1141 aattaggtga aaacgttccg cttcttatcg ataaagccgt agatttcatg gcatcaagcc
     1201 aggcgttccg ggagtatctg aaaaaacttc ctccccgtaa cgcgattccg tccggaatac
     1261 ccgatgaaag cgtgccgtta tatctacaac gtctggagta ttatcgtcgg ctttatcggc
     1321 cgaagcaggt agaggggcag taagcagacg ttgcttactt ttgaaaggct ttttttaagc
     1381 tgattttgga aatcagctca gtcagtgaaa ggaccattgt tgatcgcaca gcctgttgat
     1441 agcgctgaat ttgcattgca tacaaactgg agtctgctgg ttcaaactgt ggcggcggtg
     1501 gtaacgccgc cacgcagtgc agttcaccaa agggtccaag gatttcgtcg tcggtaaagg
     1561 cgtactcgtt gccgtcgtga tttagctctt cacgcaatgc catcagcagt tccgcatctt
     1621 cgtattcctg gcggttaatg acgcccaacc cgtaaatgag ttttaaacgc acagaaagat
     1681 cgcccagcgg accgtcgccg tcgagtaacg gttctacagc atacttcacc gcgtaatcgt
     1741 ctttgcggaa tacctgaagc accagaagat ttaccgcctc ggtcaaaagc tcgacggcgg
     1801 tgatcagaaa gcttcgcacg gttttgccag cattcagacg ctcaagcaca cggttttcaa
     1861 aggcctgggt ttgttccatt gttgcctgca tatctgacaa tctgttattc gggcgcaggg
     1921 tgtcctgcgc ctggtccacc attattgcat tgctttataa gcggttaccg cctcggatac
     1981 aacctcactg ttggcatcaa gaccggaaat ctgtgccagc gccgcctgcg gacctttgtc
     2041 agcgatcagt gctgccagtt cctgagcctg cggatcatct tcactgcgga agtgcattgc
     2101 accagcaatc ccctgaatca gattcttgtg cggaaggctg tattccagcg taccgagcag
     2161 tggcttgatc agacggtcgc cagcactcag tttacgcagc ggctgacggc ctacgcgctc
     2221 tacatcatct ttcagatacg ggttctcaaa acgaccgagg attttctgga tgtacgccgc
     2281 atgtttgtct gcgtcaaagc cgtagcgctt gatcagtacc gcaccacttt cttccatcgc
     2341 accttttacc accgcgcgga ttttctcgtc gagaatagcg tcacgaatgg tctgatgacc
     2401 ggccagtttt ccgaggtacg cggttatagc atgacccgtg ttcagggtga agagtttacg
     2461 ttcgacaaat gccatcaggt tgtcggttaa ctccatgctt gggatgttcg gcagtgcgcc
     2521 tttgaactgc gttttatcga caatccattc gctgaaagtt tctaccgtca cttccagcgg
     2581 atcgttagtt gccgaagccg aaggcggtac gatgcggtca acggcggaat cgacaaagcc
     2641 aacgtgttct tctacccacg ctttggcgtc ttccggcagg gcgttcatca catggccttt
     2701 cagctgcgtg gtaccgcgta ccatgttttc acaggcgatg atgttcagcg gggattcatt
     2761 accttgttct ttgcgtttca ccagcccttt ggcgatagcc ggagcaatac gttccagcac
     2821 aaccgggcca acggcggtag tgactaaatc gacctgagca atcagatcaa cgacatcatc
     2881 accaatgctg ctgacagcat tgacgccgga aacggtatct acctgctcgg tttcaccaac
     2941 cacatgaacc tgatagctat gacgggcatt cagggcatca agtaccacct gattgacatc
     3001 ggcaaacgtc agttggatac ccgcgtctgc cagcagttta ccgataaagc cacgaccgat
     3061 attacctgcg ccaaaatgta atgctttcat agtattaacc ttcatcaatg tttttacccg
     3121 agagggctgg ggtgaggctt ttccctcacc caaaccctct ccgtgaggag aggggcagcg
     3181 ccggatgcgg cgtaagcgcc ttatccggcc tacaagaacg tgcaaattca acaaatcgtg
     3241 atgcatcctg taggcctgat aagcgtagcg catcaggcaa ttaaccccac cttctccatg
     3301 tggagagggt gggattggat tacttacgac ctgccaacag ttccagcact tcatccacgc
     3361 tggtggtgtg tgccagacgc tcgatgacgg actcatcatc cagtgcattg gtcaggctgg
     3421 tgataacctg aatgtgctcg ttgttacggg ctgcaatacc aatcaccaga cgggcaatgt
     3481 catcttcctc ttcaccgaag cgcacgcctt ccgggtactg gcagaacacc acgcccgttt
     3541 tcagtacgcg atctttcgct tcaaccgtac cgtgtggcac cgcgatagac tcacccagat
     3601 aagtcggggt cagtttttca cgatccagca tcgcctgaac gtattccggc tcaacgtaac
     3661 cgcctttcac cagctgctcg ccagcaaaac gaatcgcttc ttctttggtt gctgctttgc
     3721 gaccgaggaa gatgttctcc gcgcccagtt tgaacaggtt agcactggaa tcgtcaaagc
     3781 tgtctttcac tttcacttcg ttttcagtat ggcgctgggc agcaaccaga cgttcggtta
     3841 ggctggtgta caggccgctg tcgaggaagt tggtcagcga aatatgctgt gcctgcggaa
     3901 cctggcgcat agcgcgttcg gtcaggtcac ggtgagtgat gacgaggtcc acatctggcg
     3961 gcaggttgtt gatcgcgctg ttagtgaccg aaatctgcga cagacctgca tcctgaattt
     4021 tcttacgcag cacgcctgcg cccatcgcac ttgaacccat accggcatca caggcaacga
     4081 tgattttacg tacgtggctc aggtcgttag tcacatcgcc cgcagacagc ggagatgtgc
     4141 ctttagactc agctttcatg tcctgcatac gacgagttgc tgcttcaata tcatcttctt
     4201 ctttcacttt gctggttttc agcaaaatag cagagacaac gaaggagaca gccatcgccg
     4261 cacacacacc cgcgatgtta gcgaaataag ccccttttgg tgtcatcgcc agtacagcaa
     4321 ggatagaacc cggagatgcc ggagaaacca gaccaccgcc caggatagtc agcgtgaaca
     4381 cgccagtcat accgccgagg atgactgcga ggatcagacg cggattcatc agcacatacg
     4441 ggaagtagat ttcgtggata ccacccagga agtggatgat tgccgcaccg cccgcagact
     4501 gtttagcgct accacgacca aagaacatgt acgccagcag cacgcccata cctggacctg
     4561 ggttagcttc aatcaggaag aagattgatt tacccagttc atgggactgc tgaataccca
     4621 gcggcgagaa gataccgtgg ttaatggcgt tgttgaggaa caggattttc gccggttcaa
     4681 caaagataga cgccagcggc agcatgtcat ggacaaccat gaagttaacg cccgcagcca
     4741 gcattttgga cagggcttca acaatcgggc caatgccgag gaatgccaga atagcgagga
     4801 tcatcccgat gatgcctgcg gagaagttat tcaccagcat ctcaaaaccg gatttgatct
     4861 taccgtctac ccagcggtcg aagtgcttaa tgcaccagcc gcccagcgga cctgcaatca
     4921 tagaaccgag gaacatcggc atgtctgcgc cgacgataac gcccatggtg gtgatggcac
     4981 cgactacgcc gccacgttcg ccgcctacca gcttaccacc ggtataaccg atcagcagcg
     5041 gcaggagata agtgatcatc ggcccgacca gcttcgccag cgtctcgttc gttaaccacc
     5101 ctgttggaat aaataacgcg gtgatgatac cccacgcgat aaacgcgccg atatttggca
     5161 tcaccatgtt gctgaggaaa cgaccaaagc tttgcacttt gatcttaata tcggatgaca
     5221 taaaaacacc ccttcttatg tttgctgtcg cgcaggctgg aagcccgagg ttttttgtta
     5281 atgtggcggc agaggtagcc ggaccttgta gatactgcga aatctggcac tgaatcggtt
     5341 aactgtccag tcgacggcct tttgtgtgat attaatcaca taaatatagg aggtagagag
     5401 gtaattgacg tgacgttcat cacaaaacgc ttgtgtaaaa aagcaacaag gcggcgattt
     5461 tcgctaaaaa tggcatttaa atgtgagtag tatcacattt ttgttttgat ggtttgttga
     5521 atctttgtga tctgaatcac aagatatttt cttacggcgt tcacctgatg tgatctacag
     5581 catgttatgc cttcgtcata caatcgttat gtaactaagc aactcatcac ctctaaaaat
     5641 tattctttgg tgttatggcg agaatcgcga acccggatta ttgtcaaaat atcagcctga
     5701 ctcccttctg gctgccagcc caaaaacaac actgataaac gtgaatccga ggcagatatg
     5761 tttctaaact atttcgcgtt gggagtgctg atcttcgtct ttctggtgat tttttatgga
     5821 atcatcgcga tacatgacat cccttatctg attgccaaaa agcgcaatca cccccatgcc
     5881 gacgctattc atacggcggg ctgggtgagc ctgtttactc tgcatgttat ctggccgttt
     5941 ctgtggatct gggcgacgct ctatcaaccg gagcgtggct ggggtatgca gtcacatgtt
     6001 gcgtcgcagg agaaagcgac tgacccggaa atcgcctcac tttctgaccg aatttcccgg
     6061 ctggagcatc aactcgccgc cgagaaaaag actgactatt ccacgttccc ggagatctaa
     6121 ttatggatct attgattgtt ttaacttacg tggcgctggc gtgggcggtc tttaaaatct
     6181 tccgcattcc ggtaaatcag tggacgctgg cgacggcggc gctgggaggc gtgtttctgg
     6241 tgagtggttt gattttgttg atgaactaca accaccctta cacttttacc gcgcaaaagg
     6301 cagtgatagc gatccctatc acgccacagg tgacgggaat tgttactgaa gtcactgaca
     6361 agaataatca gcttattcaa aagggcgagg tgctttttaa gctcgacccg gttcgttacc
     6421 aggcgcgagt tgacagactt caggctgacc tgatgacggc gacgcataat ataaagacgc
     6481 tgcgtgcgca gctcactgaa gcgcaggcca acaccaccca ggtttcagcg gagcgcgacc
     6541 gtctgtttaa aaattatcaa cgttacttga aaggcagcca ggcggcggtg aatccgttct
     6601 cggaacgtga catcgacgat gcgcggcaaa atttcctcgc gcaggatgcg ctggtgaaag
     6661 gctcggtggc ggagcaggcg cagatccaga gccagctcga cagtatggtt aacggcgagc
     6721 aatcgcagat tgtgagctta agagcgcaac ttactgaagc aaaatataac cttgagcaga
     6781 ctgtcattcg cgcgccgagc aatggctacg ttactcaggt actgatccgc ccaggtacat
     6841 acgcagctgc cttgccgctg cgtccggtga tggtcttcat ccccgagcaa aaacggcaaa
     6901 ttgtcgccca atttcggcaa aactcgctgt tacgtctgaa acccggcgat gatgcggaag
     6961 tggtgtttaa cgcgctacct gggcaggtgt ttcacggcaa actgactagt attttacctg
     7021 tcgtgccagg cggttcttat caggcgcagg ggggattgca atcattaacg gtcgtgcccg
     7081 gcacggacgg tgtgcaggga accattgaac tggaccctaa cgatgatatc gatgccttac
     7141 ccgacggcat ctacgcccag gtggcggttt actccgacca tttcagccat gtttcggtga
     7201 tgcggaaagt gctgctaaga atgaccagct ggatgcatta tctttatttg gatcattgag
     7261 ggggaggggg gagattatgg aactttgctc cttgtaagga ctaattattt ttgcatatta
     7321 cttcattagc tgcgatgatt tttctttaat attcattatg ttaataagtt ctggatgagt
     7381 gtgctctcat tggatttttt atccgtcttg ttctgccgca ggatgagact atacatcgtc
     7441 gagatcgcaa tgatgatatg agttgttcct cctgcgacgg ctctaatcaa aaaagagcag
     7501 gagtgctttt ccatctattg cgatgatggc tttattatct gaaggatgac agtcacattt
     7561 aaaaaatatt taatctatag atcacaatgt agggtcatgg aatatatgta tacaactatt
     7621 ttcttcttca cttttaatta tcgtattcat tataaaacta agttaaaaaa actcctggag
     7681 agtgagttat taatatattt ttaaataaaa ttaagttatc attcatcgat gcattttata
     7741 gaatcatcaa tggccataat agtactatta actctcaatt ttatattttt atcatcacta
     7801 caaatattaa ctgaatttat atcaatgatt tttttattct tatcttttat tttatttact
     7861 gtttcaatgc aatcagcggc aagttggagt ggatttagat ttttcagata tggtcttatg
     7921 atctttccct tacttaataa atagcatgta taggattcgg aaagtccagc atctattttg
     7981 tacctcatta tattagataa ggatgtgaga aattccttgt tatcattcgc agccatatat
     8041 gccagtccga gttcagcctc tgcggcctta caggcaaaat agcttagtga acacgctttt
     8101 cgttcctctt tagtactggc tgatattagt gcccataata ttgctttgtc tgccaaatct
     8161 aactctctct gaaattgtct tgcagtaact gcactcgcag aaatagagaa aaaaatacaa
     8221 ttgttaataa ccttgccata tttttttagt ccgtaactgt taatgtgcta tctggctctg
     8281 aaaatattaa ctcatttaat ttttgcatgt cattagataa accagttata caaccctcgg
     8341 aacagaatcg aggtcctaaa gacggaccaa aagcgtttaa acaactatgt attgaaaatc
     8401 cattgcgatt ataagttcct gtattaactg acggtactaa tcgtatgccg tcaggttttc
     8461 tattgcttgc tcccgggtca ttaacattcc aactccatag gccttgaggt attggccatc
     8521 tatttataaa tgtgtacaac ggggattatt tttacattgc atattattac cagaagcgac
     8581 tggaatacta agcacatcat aaaaattatc atcacatggt atatctgaaa cacacgaaag
     8641 catacctaaa gttatgctgt ataaacaggt ggctaatccc aaggggatcg attccagaaa
     8701 tcgggttcaa cggatactga taaaaattcc acctaccttc cagcccaatc ggagcctgag
     8761 tgttataccg cccctgctgc ggttaatgtt accgataata gttatagtac agccctgatt
     8821 catcatcata ctactgctgc ggcagtctga tgaactattg cttttacgga ttctccttat
     8881 tcttccttct atccattcgc tttagctttt tgagctcttg aagtttgctc tttgcatcaa
     8941 tattaccgtt atttacagcg aattccatcc aataaatagc tctattcaaa ttataatatt
     9001 ctgataatgt tggatttgta tctgataaaa agaccccata attaaactgc gctgtaacat
     9061 tcccctgaga tgctgatctt tccaagaatc gcaattgttt atcaatatta tttttagtaa
     9121 agcagtaata ttgatatagt cgaaatgatg cctctgaatc accatgttgt gatttatact
     9181 tcagtttcat cagcttttct tcacttaaat cataggtcat tgctgtagat cccatcacac
     9241 tttcgttttc ttttattttc acatcagcac catgcaattg atcaataaga gaaaaataaa
     9301 aaaatagtaa caagcatgct ttcatacatc tacaacctat cttatatcac tgggtttttc
     9361 tggcgtacct tgcatattag aaatggggtg cccagatggc ccatatgtta tccaattacc
     9421 aaggtctttt gttgttatag ccattagatc cttatttgaa ttaaggttcg ttggtgctgt
     9481 tagcactgta tccattttta acttaccatt agttcctcgc ccccaccacg tccatagcgt
     9541 gtcaggtcct attacatttg tatgtaactc tttagctaat tggctagcaa atgaattttg
     9601 tcctttactt gtattacaag aaaataacca aactgtcatc ccatctttat aattcccatc
     9661 aaatttaatt agatatgcta gatctttagc tgtcatgata ctgcgcgttg cggattcaat
     9721 agatgtgggg gtaccatgcc ccccgattgt gaaaacgcct gggatattaa tctcatcagc
     9781 tacagaatgg ataagatcac tttcggggta taaatttata tcaaccaaac cttgcggatc
     9841 tacatttatg accggattca acggatactg ataaaaattc catcccccct tcagcccaat
     9901 cggatcctga gtgatatacc gcccctgcag cgggtcataa tagcggtggc ggttgtaata
     9961 caggccggac tcctcatcat actgctgacc cggcaggcga atgagctgct gcagatgatg
    10021 cgggttctca tcactcagca ggttgctcca ttcatcatat tctgcgcacc acgctgttgc
    10081 cccttccgtg ctgatgagcg ccagcggcag gccgcgatgg tcgcagtggt acaggtggat
    10141 ttttcgcgcc ggcgtgta
//
LOCUS       DAJVUJ010000154        10146 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.154, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000154 DAJVUJ010000000
VERSION     DAJVUJ010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10146
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.154"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..236
                     /locus_tag="OQQ48_003435"
     CDS             <1..236
                     /locus_tag="OQQ48_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCS1954081.1"
                     /translation="TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLV
                     YEKTYTAELSSIAGTPIEAGPFDTVVLFKINYN"
     gene            388..1434
                     /locus_tag="OQQ48_003436"
     CDS             388..1434
                     /locus_tag="OQQ48_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCS1954082.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTCM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPDGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(1431..2222)
                     /gene="nei"
                     /locus_tag="OQQ48_003437"
     CDS             complement(1431..2222)
                     /gene="nei"
                     /locus_tag="OQQ48_003437"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HCS1954083.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVGTRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(2258..2992)
                     /gene="pxpA"
                     /locus_tag="OQQ48_003438"
     CDS             complement(2258..2992)
                     /gene="pxpA"
                     /locus_tag="OQQ48_003438"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HCS1954084.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSTFAEKEIVVAA"
     gene            complement(2982..3914)
                     /gene="pxpC"
                     /locus_tag="OQQ48_003439"
     CDS             complement(2982..3914)
                     /gene="pxpC"
                     /locus_tag="OQQ48_003439"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HCS1954085.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLNVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSPQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(3908..4564)
                     /gene="pxpB"
                     /locus_tag="OQQ48_003440"
     CDS             complement(3908..4564)
                     /gene="pxpB"
                     /locus_tag="OQQ48_003440"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HCS1954086.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIELLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(4587..5330)
                     /gene="ybgI"
                     /locus_tag="OQQ48_003441"
     CDS             complement(4587..5330)
                     /gene="ybgI"
                     /locus_tag="OQQ48_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HCS1954087.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            5601..7082
                     /gene="dtpD"
                     /locus_tag="OQQ48_003442"
     CDS             5601..7082
                     /gene="dtpD"
                     /locus_tag="OQQ48_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HCS1954088.1"
                     /translation="MNKHASQPRAIYYVVAPQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGSFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGTIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(7124..8542)
                     /gene="phrB"
                     /locus_tag="OQQ48_003443"
     CDS             complement(7124..8542)
                     /gene="phrB"
                     /locus_tag="OQQ48_003443"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HCS1954089.1"
                     /translation="MTTHLVWFRQDLRLHDNLALAAACSNSSARVLALYIATPRQWAT
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIEPSPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHCPFIAWTDRVQWQSNPAHLQAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVMLDYPQP
                     IVDHKEARLRTLAAYEEARKGA"
     gene            complement(8539..9048)
                     /locus_tag="OQQ48_003444"
     CDS             complement(8539..9048)
                     /locus_tag="OQQ48_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HCS1954090.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDYYNQYRQRVVVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            complement(9333..9707)
                     /locus_tag="OQQ48_003445"
     CDS             complement(9333..9707)
                     /locus_tag="OQQ48_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1954091.1"
                     /translation="MFTKLSIAILLAFSAYVHAETSAPIFFEDVGSIDSWFSIKYQKY
                     ENVKDEQYPEILIYYGVKGSGINRVDGWFFSCAKAGRTCSLIFMADFGESRYFKPAKI
                     AMNKNNLIIRTSDVEIAFKITK"
     gene            complement(9692..>10146)
                     /locus_tag="OQQ48_003446"
     CDS             complement(9692..>10146)
                     /locus_tag="OQQ48_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCS1954092.1"
                     /translation="LGLIDKPAPVFNRDLNSDPYYLAVNNCYSYALNRYGNPGSRIFG
                     GGGLQPGELSGKEFSKLTCGSIFEASKNDGAKDLDNGSCPSGYHKAQLFIRPHNFIGM
                     GGDYHWYRQDANGEWSDKQGVGAIRFRGKDPFPPIDYPEKCGTIFLPN"
BASE COUNT         2367 a         2754 c         2437 g         2588 t
ORIGIN      
        1 aaacgttggt tgaagaagat aaagcattgt tgattggtaa tggtctgaag ctacgtttgc
       61 tggatgaaaa cgcctcgcct tataccttca ataaatatgc tgaatacgcc gatttcacca
      121 gcgacatgtt ggtctacgaa aaaacctata cagctgaact ttcgtccatt gcaggtaccc
      181 ccatcgaggc tgggcccttc gatacagtgg tgctttttaa aattaactat aactgatttg
      241 ccatgatgcc ctttgcagcc ggaaagggca tctctttcag aaaattgctg attttcctct
      301 tttccttgat tactgttatt ttttcctggc ccggctttct ttatgctgta tcgatacacc
      361 aaagagaaaa taatgaggga gcgaaggatg ccagttttgc agtggggaat gttatgtgtg
      421 ctatcacttc tcctttctat tggtttcctc gcggtacatc tcccggcggc gctattactt
      481 gggccgatga tcgccgggat catcttcagt atgcgcggaa ttactctgca acttccccgc
      541 tccgcttttc tcgccgcgca ggccattctt ggctgcatga ttgcgcaaaa cctcactggt
      601 tcgattctca ccacgctggc cgccaactgg ccgatcgtgc ttgctatttt actggtgacg
      661 ctgctttcca gcgccatcgt gggttggtta ttggtgcgct atagctcact gcctggaaat
      721 accggtgcct ggggttcttc tccaggcggc gcagcggcaa tggtcgctat ggcgcaagat
      781 tacggcgcag atattcgcct ggtggcgttt atgcaatatc tgcgagtgct gttcgtcgcg
      841 ggggccgctg ttctggtcac ctgcatgatg ctgggtgata acgctgaagc ggttaaccag
      901 caaatcgtct ggttcccgcc agtgagcatt aatctcctgc ttacgatttt gctcgcagtc
      961 gttgccggta cggcgggatg catgctgcgt cttccttccg gcacgatgct catcccgatg
     1021 ctggcgggcg cagtgctcca gtctggtcag ctcatcacca tcgaactacc ggaatggctg
     1081 ctggcgatgg cgtatatggc aattggctgg cggattggtc ttggtttcga taaacaaatc
     1141 ttactgcggg cactacgccc actaccgcaa atcctgctgt cgatttttgc tctgctggct
     1201 atttgtgcag gtatggcgtg gggactgacc cggtttatgc atattgattt tatgactgcc
     1261 tacctcgcca ccagtcccga cgggcttgat accgtggcgg tcatcgccgc agggagcaat
     1321 gccgatatgg cgcttattat ggcgatgcaa accctgcgcc tgttcagtat tttgctgacg
     1381 gggccagcca ttgcgcggtt tatttcaacc tatgcgccga agcggtcggc ctagtgctgg
     1441 cagcctggac accagtaaaa cgggcgcgag gacaacgtgg ttttctcaat gatgccgcca
     1501 cagcgttcgc acagttcgcc atcacgatga aaaaccttaa agcgaaacag cgccccatga
     1561 tgcttattct catccacctg cccccgcgta gcgtaagaaa accgaggaat ctccagtaac
     1621 gcgtgtgcga gtgcatccag ttgcgccgca ttgagatctt tcgctttatg atttccagtc
     1681 aacccaacct gccagaggat ctccacccgc aaataattgc caagcccagc cagaaacgcc
     1741 tgatcgagca gtaatcctgc aaactgacgg ttacgaaagc gcggtgacaa taaccgttcc
     1801 tttaccacct ccggcgtcag attcggatcc agcacatcgg gaccaacgcg ttgtaaaaac
     1861 ggatgcgtgg tcagttgttc cggcctcaac atctcaatat ccgaggcgct ataaagcaga
     1921 atggttttgt cagccgtttg cagttttacc cgcaatactc gcgtggtctg cggctcttcg
     1981 ccggtatcaa ccacgcgcca gacgccgtaa agctgattat ggctgtagag cgttaagtcg
     2041 ttggaaaaat gagttaacaa cgccttacca cgcgttccca catgggtaac gtgttgaccg
     2101 ataagttgtg attgataagt ttttaactgc gggaaggcaa accagacatc agttagtggt
     2161 ttgcctttga tcgccgcctc caggttatcc gctgcacggc ggatctccgg gccttcaggc
     2221 atggtgatat ccttttttca ttctttatta atcggggtta tgctgcgaca acaatctcct
     2281 tttcggcaaa tgtagagcgt agtcggcggg cgaacgccag tgcgtgctcg ccgtcgccat
     2341 gcaggcagac tgtttgcgcc gtgaccgttg cccattcgcc ggtgatactt ttgactctgc
     2401 cgtgttgcac catctctagc gtttgcgcca gcgcctgttc ttcgttttca atcaacgcgc
     2461 ctggctgact tcgcggcacc agcgagccgt cagcctgata accgcgatcg gcaaacactt
     2521 cctcgcgcgt tgtcaggcca tattgcttgc ctgcacgaat cagctcgctt cctgccagcc
     2581 caacgagaac caatgctgga tcgcaagcgt ataccgctct ggcgatggcg tctgccagtt
     2641 gtgcttcttt cgccgcctgg ttgtacaaca tgccgtgcgg tttgacatga cgcattacgc
     2701 cgccttgcgc acgggcaatc gttgccagcg cgccaatttg atacagcgtc tgggcgtaaa
     2761 cggtttctgg cggcagctgc atggcgctgc gaccaaaatt ttccctgtcg ggaaaactcg
     2821 ggtgagcgcc aatcgcgaca ccatttttta ttgcttcacg cacgcaagcc tgcatggttt
     2881 gggcatcgcc tgcatgaaat ccacaagcaa tattggcaga ggaaaccagc gttaatagct
     2941 ctgcgtcgct ggcgcagcct tcgcccagat cggcgttcag gtcaattttc attgtgtagc
     3001 cgccacgcta attgttcgaa ataacgttgt tgatcttgcc gcgctttcag tgcctcttcc
     3061 agtgaacact ggacaaaatg aatcggctga ccgagcggaa tttgcgccag atggtacata
     3121 tcagcctcaa tgatacaggc aatacgcggg taaccaccgg tggtctgtgc gtcgttcatc
     3181 aacacaatcg gctgcccgtt atgcggcacc tgcaccacac ccggtaacaa accgtgagat
     3241 aacagttcgc gatcggtggt gcgttttaaa atttgcccct gtaagcgata gcccatgcgg
     3301 ttactttgcg ggctaagctg ccagggcgaa cgccagaatg catcctgcga ggcgcgatcg
     3361 aactcatgat attccggccc cggcaaggcg cgaatgcggt tgccccacag cagctgttta
     3421 acgccctgcg cttccataaa atcacgcttc gatttgccaa tcgggagtcg gtcaccatcc
     3481 ttcagtaaac ggccttccag cccgccaatc cccacgttga gatcggtgct gcatgacccc
     3541 attaccggcg gaacatcaat accacccgcg accgccagat aactgcgcat gccgtgctgc
     3601 gggcgtttaa gcgttaaacg ctggcctgct ttcatcggca agcgccagcc ggtccagacg
     3661 gcgttatcat ccagccgcgc ttcgcaaccg gcacccgtca gagcaaacca cccatcagtt
     3721 tcgaactcaa ccgttaactg accgagcgtg atctccagcg cgggggcatt ggcgtcatta
     3781 cccaccagca ggttagcaat gcgtaacgca ggcatatcca gtgcgccgca gtggctgata
     3841 cccgactggc gaaaaccgtg acgaccgcca tcctgcacag tggtatacat gcccgcacga
     3901 ataatcttca gcatactccc tccttctgcg gtacaaagcg cacgctgtct cccggacgta
     3961 ataagatggg ttcgtcacgc gccggatcaa acaggctgag tgaggtatga ccaatcaact
     4021 gccagccacc cggcgttgcc agcggataaa caccagtctg cggcccgccg atcccgacag
     4081 aacctgccgg aacgagcaag cgcggttcag cgcgccgtgg cgtgtgtagt tgttccggca
     4141 aactcccgag atacgggaag cccggttgaa aacctaaaaa ccagaccacg tattccacgg
     4201 aggagtgcaa ttcaacaacc tgtttttcgc tcaacccgca atgcgccgcg accaccgcca
     4261 aatccggtcc gcctgcacca ccgtaaacca ccggaatttc aataaagcga gactcaggtt
     4321 ccagggcctc gctctcctcc caccagcgtt gcaaaagctc tatggcatcc agcgccagcg
     4381 actcaggatt acgcaaaatc accgtgatat tgttcatgcc gggaatggct tcaaccacat
     4441 tcggcatatc caccagacgc tgcgccagtc gccagatccg tttctggcta gccagcgtca
     4501 ccggcggttc cagttccagc actaccgccg tttcacctat cagataacaa cgcgctcgtt
     4561 gcactttcga tccctctgat tattcgttat gcaggattag gaatatcaat aaaggtcaca
     4621 tcaagatcgg tattttcatt cagccactcg ctcaatgcgc gaataccacc acgttcagtg
     4681 gcatggtgac ctgcagcata aaaatgcaat ccctgctcgc gggctgaatg aatggtctgt
     4741 tcagaaactt cgccagtaat aaaagcatcc acgccaaaac gcgcggcgct atcgataaaa
     4801 ctttgcccgc cgcccgtgca ccaggcgacg cgctggacca cctcaggtcc ggtatcgcca
     4861 caccataacg gcttacgtcc cagacgcgct tcaatccagg aggctagttc cagtcccggc
     4921 acaggcatgg tcagctctcc ccacggcacc aacggctcaa tttcgcccat gaccgtgatc
     4981 cccagtaacg ccgccagctg tgcgttattg cccagctcag gatgtgcgtc aagcggcaaa
     5041 tgccagccat acaggttgat atcattcgcc agcaacgttt ttaaacggtt acgcttcatt
     5101 ccgcgaatga ccggagactc tcctttccag aagtagccat gatgcacaat gaccgcatcc
     5161 gcgcccagac gcactgcctc atcgagcaaa gcctggctgg cggtgacacc ggtaacaatt
     5221 ttttgcaccg tctctttgcc ttccacctgc aaaccattcg gcgcgtaatc actaatcgcc
     5281 gcgctgttca gtttttcgtt aatcagttgt tccagttcgg tgtttttcat cttcgcgcct
     5341 tccgtattct gatagctaca ccgttaacat agcgttacct cgcctgttcg tcgatagcca
     5401 atcttttgac atattaagaa acaaacccga cgccaaaaga cgcaaattgt ttatatcatc
     5461 acgcttcagc taatgtatat ttatgcaatc agacagcata ttgcataaat aatcagatct
     5521 gtatcaaagt tttaacttta aggaaacaca agcacgccgc gcaactatta cgtgacgttg
     5581 ccgtcaattg tggaattatt atgaataaac acgcatcaca gccgcgcgct atttactatg
     5641 tcgttgcgcc gcaaatctgg gaatatttta gcttttacgg catgcgtgcc ctgctgattc
     5701 tctatctcac caatcaacta aaatacaacg atactcacgc ctacgagtta tttagcgcct
     5761 actgttcgct ggtgtatgtc acgccaatcc tcggtggctt tttggcggat aaagttctcg
     5821 gcaatcgcat ggcggtgatg ctgggggcgt tattgatggc gatcggtcat gtggtgttgg
     5881 gtgccagtga gatccatccg tcattcctct atctgtccct ggcgataatt gtttgtggct
     5941 atggtctgtt taaatccaat gtcagttgcc tgctcggcga gctgtatgag ccaaccgatc
     6001 cgcgtcgtga tggcagtttc tcgctgatgt atgcagcggg taacgtgggg tctattatcg
     6061 cacctattgc ctgtggctac gcccaggaag agtacagctg ggcgatgggc tttggcctgg
     6121 cggcggtggg tatgatcgcg ggtctggtca ttttcttatg tggcaatcgt catttcacac
     6181 atacccgcgg cgttaataaa aaagtactgc gtgcgacaaa ctttctcctg cctaactggg
     6241 gatggctgct ggttctgctg gtggcaacgc ctgcgctgat taccgtattg ttctggaaag
     6301 agtggtcggt atacgcctta attgtcgcga ctattattgg cctgggtgta ctggcaaaaa
     6361 tttatcgcaa agcagaaaac cagaaacagc gtaaggagct ggggctgatt gtgacgctca
     6421 ccttcttcag tatgttgttc tgggccttcg cacaacaggg cggcagctcg attagccttt
     6481 atatcgaccg cttcgttaac cgcgatatgt ttggttatac cgttccgact gctatgttcc
     6541 agtcgattaa tgccttcgca gttatgctgt gcggtgtgtt cctggcgtgg gtggttaaag
     6601 aaagcgtcgc aggtaatcgt accgtgcgca tctggggaaa atttgctctt ggtctcggcc
     6661 tgatgagcgc cgggttctgc attctgacct taagcgcccg ctggtccgca atgtatggtc
     6721 actcttctct gccactgatg gtattaggcc tggcggtgat gggctttgcg gaactgttta
     6781 tcgacccggt tgccatgtcg caaattacgc gcattgaaat ccccggtgtg accggcgtat
     6841 taaccggcat ttatatgctg ctttccggca cgattgcgaa ctatctcgcg ggcgtgattg
     6901 ccgatcagac atcgcaggct tcgtttgatg cttccggggc gatcaactac tccatcaatg
     6961 catatattga agtatttgat caaattacct ggggcgcact ggcgtgtgta ggactggtac
     7021 tgatgatttg gctgtatcag gcgctgaaat tcagaaaccg cgcgctggcg ctggagtcat
     7081 aataggccag caccatacct gataaaacgc tcgctgcgtt ttatcaggct cctttcctcg
     7141 cttcttcgta cgctgccagc gttcgcagcc ttgcctcttt gtgatcaact atcggttgcg
     7201 gataatccag catcacacct gctttctgcg cccacttcca cggctcatgc accgctttcc
     7261 ctggtacatc gcgcagctcc ggtaaccatc gacgaataaa ctcgccctca cggtcaaatt
     7321 tctcgccctg ggttgtcggg ttgaaaatac gaaaatacgg cgctgcatcg gttccggttg
     7381 aagcggccca ctgccagcca ccgttattgg ctgccaaatc accatcaatc agctgcgaca
     7441 tgaaatatcg ctcgccttcg cgccagtcga tcaacaaatc tttcaccaga aaactggctg
     7501 taatcatccg tagccggtta tgcatccagc cagtgctgtt aagctgacgc atggcagcat
     7561 cgacaatcgg gtatcccgtt ttgccttcct gccaggcctg taaatgtgcg ggattgctct
     7621 gccactgcac acgatccgtc caggcaataa acggacaatg tttacacaac gaggggtaat
     7681 acgtcatcag atgacggtag aactcgcgcc agatcagctc actaagccag acactaccgg
     7741 ccccaccgtc cagcgcctgc ggctgttccg ccaacaagcg atgcaaacac tggcgaggcg
     7801 ataacccgcc cgttgccagg ctggcggaca aacgactggt gccttccact gccggaaaat
     7861 ctcgttgttg ctcatattct ccggcaccgt tctggcaaaa ctggcgtaat tgcgcaatcg
     7921 ccgctttttc ttccaccggg aaatgcgcag tatcgaaaga ctgacgagga taattcagcg
     7981 taatggatgg cgagggctct atcgatccgc tactacgaac ttttggcgca gcgacgcact
     8041 ccggcatccc ttcccgcagc cgtttcagcc aggcattctt aaaaggcgta aagactttgt
     8101 acatctcgtg attaccggtc atcaccgcgc caggcggcag gatcacgctg tcatcaaatc
     8161 cttcacacac cacgttacgc agcgctcttt caacttccac atcccgcgcc cgctcattca
     8221 cttcatactg atagttataa aacaggtggg taacgctgtt ttccgcgcac acctgtttaa
     8281 ctatttcgac actggcgaca aagtcatcca cttcacggaa caataaagga ataccttttt
     8341 ccgcaagcgc tatttgtagc ccattcagtt gagcattgat aagttcagcc tgacgcggcg
     8401 acatgttatg cgtcgcccac tggcgtggtg tagcgatata caacgccagc acgcgtgcag
     8461 acgaattgct gcaggcggca gccagtgcga gattatcgtg caaacgtaaa tcctggcgaa
     8521 accagaccag atgggtagtc ataaaactcc tgaatggcgc aagttaatca cacgcggcca
     8581 aagttcgaaa taacgctgcc ctgaaagcca ggcgtcagga taaagcgcca tatagtgttt
     8641 gatacgcatc agcggcgcaa gaagtggttg ctcgccacgg cgataactgt cgataagcgc
     8701 agccagctgc tggcgttctg tggaatcaat atgcgggcga aaataaccct gaacgtgcat
     8761 caaaacattg gtgtgatcgc gcgggttggc ggggtgagaa agcaaaacaa ctacgcgttg
     8821 gcggtactgg ttgtaatagt catccagatt ttgccactgg tgaatatcag ccacgaacgg
     8881 gccaagtttg cggtactccg gctgagaatg cgccagaaag acgagtttat agcggctgtg
     8941 ataatcgaga agcgccccgc gcgttaagcc atgttctttc agtcgatgta actcatgaag
     9001 ggcaaagata cggattaggg tgctgtcatc aaatcgttgt agattcatta ctgctcctga
     9061 acggtataaa ggagtaagtg tagacgatgg taaatgaatt ttatggggca gagtttttgc
     9121 ggggaagtat tttgttggta aaaatattat caacttcatc tgagcgagaa ttattctact
     9181 atttcccgag ctgcattcat tttttgtatt ttttattttt agaaaataac agttctcaaa
     9241 gcttaattac ctcactatta attgtatttc aaactaacaa tcgcactcca ttttctctct
     9301 ttaaaaaaca taaacattgt taaaacaaat atttatttcg ttatcttaaa tgctatttct
     9361 acatcactcg tccttataat caaattattc ttattcatag ctatttttgc aggtttaaaa
     9421 tagcggcttt caccaaagtc agccatgaaa attagactac aagttctccc agcctttgca
     9481 catgagaaaa accacccatc aactctattt attcctgaac ctttaacacc ataataaatc
     9541 agaatttctg ggtattgttc atccttcacg ttttcatatt tttggtattt aattgaaaac
     9601 caagagtcta tactgccaac atcttcaaaa aatattggag ctgatgtttc cgcatgaaca
     9661 tatgcagaaa atgctaacaa aatggcaata cttagtttgg taaaaatatc gttccacact
     9721 tttcaggata atctattggg gggaagggat cttttcctct aaatctaatt gctcccactc
     9781 cttgtttgtc agaccattcg ccatttgcat cctgacgata ccagtgataa tcccctccca
     9841 tgcctataaa attgtgagga cgaataaaca attgagcttt atggtaacca gatggacaac
     9901 tcccattatc taaatccttg gcaccatcat tttttgacgc ttcaaaaata ctaccgcaag
     9961 tgagtttaga aaactctttt ccagataatt ctccaggttg taatcctccg ccgccaaaaa
    10021 tcctactccc agggtttcca tacctattta gagcatatga ataacaatta tttacggcaa
    10081 gataatacgg atctgaattt aggtctcgat tgaatactgg agcaggttta tcaattaagc
    10141 ccagcg
//
LOCUS       DAJVUJ010000155        10119 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.155, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000155 DAJVUJ010000000
VERSION     DAJVUJ010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10119
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.155"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            206..358
                     /gene="hokA"
                     /locus_tag="OQQ48_003447"
     CDS             206..358
                     /gene="hokA"
                     /locus_tag="OQQ48_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HCS1954093.1"
                     /translation="MPQKYGLLSLIVICFTLLFFTWMVRDSLCELHIKQGRYELAAFL
                     ACNLKE"
     gene            complement(546..758)
                     /gene="cspA"
                     /locus_tag="OQQ48_003448"
     CDS             complement(546..758)
                     /gene="cspA"
                     /locus_tag="OQQ48_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HCS1954094.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(1039..1329)
                     /locus_tag="OQQ48_003449"
     CDS             complement(1039..1329)
                     /locus_tag="OQQ48_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HCS1954095.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGISVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            1762..2472
                     /locus_tag="OQQ48_003450"
     CDS             1762..2472
                     /locus_tag="OQQ48_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HCS1954096.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQTDDLKPVFDQAFTKVVTTP
                     ADALQPLIPVAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2522..3496)
                     /gene="ghrB"
                     /locus_tag="OQQ48_003451"
     CDS             complement(2522..3496)
                     /gene="ghrB"
                     /locus_tag="OQQ48_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HCS1954097.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKPSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3600..4259)
                     /locus_tag="OQQ48_003452"
     CDS             complement(3600..4259)
                     /locus_tag="OQQ48_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HCS1954098.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLDSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            4412..6745
                     /gene="bisC"
                     /locus_tag="OQQ48_003453"
     CDS             4412..6745
                     /gene="bisC"
                     /locus_tag="OQQ48_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HCS1954099.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVHVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKTAEWAAGICGVHAAKIRELAALFHQNTTMLMAGWGMQRQQLGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRCSAVLSSMQGSLPGGSDAVDK
                     IPVARIVEALENPGCAYQHNGMNRLFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSELQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPQAHPLKTASGKIEIFSQRIADYAYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDALARGIQD
                     GDTVRLWNARGQILAGVVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6714..7154)
                     /locus_tag="OQQ48_003454"
     CDS             complement(6714..7154)
                     /locus_tag="OQQ48_003454"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCS1954100.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDSKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRHPHLML
                     EVYQKNQPAINFYQAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7151..7714)
                     /gene="tag"
                     /locus_tag="OQQ48_003455"
     CDS             complement(7151..7714)
                     /gene="tag"
                     /locus_tag="OQQ48_003455"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCS1954101.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTPASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            7872..8570
                     /locus_tag="OQQ48_003456"
     CDS             7872..8570
                     /locus_tag="OQQ48_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCS1954102.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            8798..10000
                     /locus_tag="OQQ48_003457"
     CDS             8798..10000
                     /locus_tag="OQQ48_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1954103.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSDNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHI"
BASE COUNT         2427 a         2434 c         2591 g         2667 t
ORIGIN      
        1 tttttttagg attatatcaa ggagaagaaa caaacttatt aagctagaat agctacgggt
       61 gcttgagact gtttgtctca ggtattcacc gaaaggcaga cagagaaaag ccccacctga
      121 ctataaatca aagtgaggct accctatgcc tgaacaccat aaggttagcc tcttactcgt
      181 tggaaatcaa cacagggggc tgggaatgcc gcaaaaatac ggattacttt cattaatagt
      241 gatttgtttc acgcttttat ttttcacctg gatggtaaga gattcactgt gtgaattgca
      301 tattaaacag gggcgttatg agctggcggc atttttagcc tgcaatttaa aagagtaaga
      361 gtcttcggcg ggaaattatt cccgcctttc ttacggcgtt gcgcattctc attgcaccca
      421 catttattct tcacaaaaat aataatagat tttactacgc gatcgattat ttatttcctg
      481 aaaaaaaata aaaaaaatcc ccgccaaatg gcagggatct tagattctgt gcttttaagc
      541 agagattaca ggctggttac gttaccagct gccgggcctt tagcgccgct ttcgatggtg
      601 aaggacactt tctgaccttc gtccagagat ttgtaaccat cgttctggat agcagagaag
      661 tgtacgaaca catctttaga gccatcgtca ggagtgatga agccgaagcc tttgtcagcg
      721 ttgaaccatt ttacgatacc agtcatttta ccggacatag tgtattacct ttaataatta
      781 agtgtgcctt tcggcgatat ggcgtgcttt acagattttg aagcgttaaa ggaatgtgca
      841 ctacgagggg tatcaacgat aactcttgaa gggacttgcc ttactacact gctttaatgg
      901 tctgtacgtc aaaccgttga tgtgcattaa gccacgcatt ggcgggtgat gcaacaatta
      961 tttttcatat ttatgattaa tcggttggaa aacggtcctg tcatcaggac cgtaaacagc
     1021 aataaagtgg ataaaagtct attccatcaa ctgcttactt aatgccgggt tggcttgaat
     1081 caaacgcatc aattttagtt cggcacttga aggcttcacg cgtctggatt cccattcctt
     1141 taccatggcg actgatatgc ccaaaacccg ggcgaaatca tcgattttta accctgtccc
     1201 ttttcgtaac tgctcaattt cggtacagga cgttgttctg tgcgtcaggg taattttctg
     1261 cgtttcatct ttaaaaacaa tctgttccag gctgctcaac agctcatgca ttggatcttt
     1321 atattccatt gaaaactcct caaatcacac tgcgggatcg tgaattgcat cgaagctcat
     1381 taagaatagt cgggaaaagc agacacagcg gggtgaagtc agggcatgct ctggctatga
     1441 ttcgattcag ccagtcattt agcgtgactt atagccacct aacgatctga taaatgccga
     1501 tatatctctt accactcctg ttttgtaaag atttcgaaaa tctaaagttc tgcgaaggcg
     1561 taaatctcgg gctacaaaaa agcaacggca atatcatttt ttaacaatga aacaacaaat
     1621 taaccttgtt tcattttctc catgatttta ccaggaagat tacgtagaaa gggtatcctg
     1681 caagcctgtc ctggactatc cttgtcacgt cagacacgcg tgtgtcgttg tgcgcttttt
     1741 ttgggtgaaa ggagtaagaa aatggcgaca ggaaagtcct gctctcgctg gtttgcgcct
     1801 cttgcggcgt tattaatggt agttagcctg agtgggtgtt ttgataaaga aggcgatcag
     1861 cgtaaagcgt ttatcgactt cctgcagaat acagtgatgc gtagcggtga acgtctacca
     1921 accctgactg ccgatcagaa aaaacagttt ggtccttttg tctctgatta cgcgattctg
     1981 tatggttatt ctcagcaggt gaatcaggcg atggattccg gtctgcgccc ggtcgtagac
     2041 agcgttaacg caattcgcgt accgcaggat tacgttacgc aaagcggtcc gctgcgtgaa
     2101 atgaacggtt cgctgggtgt gctggcgcaa caactgcaaa atgcgaagtt gcaggccgat
     2161 gctgcgcact ctgcgctaaa acagaccgat gatttgaaac cggtctttga tcaggcattc
     2221 accaaagtgg taacgacgcc agctgatgca ttgcaaccgt tgattccggt tgcacaaact
     2281 ttcacgcaac aactggtgat ggttggagac tacattgctc aacaaggtac tcaggtgagc
     2341 tttgtagcaa atggtatcca gttcccgact tcgcagcagg caagtgaata taacaaactg
     2401 attgcgccgt taccggcaca acatcaggcg tttaatcagg cctggactac agcagttact
     2461 gcaacccaat aaagagtaaa agcccgagcg attatcgggc tttacttcac gcagtcgcgg
     2521 cttagtccgc gacgtgcgga ttcacacagt tcttctcaac ctttccttgt aacgcatcaa
     2581 tcaaattatc cacggcacag gcggccatgc cataacgcgt ctcatgggtg gcagatccaa
     2641 tatgcggtac tgcgacgacg ttggccattg agagcaacgg cgaatctacg gacagtggct
     2701 cttgttcgaa gacatccagc ccggcggcgt gaatttcgcc tttctgcaat gctgcgatca
     2761 gtgcattttc gtcaaccacc gggccacgtc cggcattaat gaaaatggcg gagggtttca
     2821 ttttggcgaa ttgttctgcg ccaaacagat gatgcgtctc atcagttaac ggcaggatca
     2881 ggcaaacgaa atctgactct tgtaacagag tatccaaatc gcagtagcgg gcgttgaagc
     2941 gttcttctgc ttctttatgg tggcggcgcg cgttatagag gatgggcatg ttgaagccaa
     3001 agtgcgcacg ttgtgccagc gccatgccga tccgtcccat cccgacaatg cccagtgttt
     3061 tatggtgaac gtcagtgccg taccagtccg ggcctatgct cgcggtccat tcgcctgctt
     3121 ttacccgttc tgctacctcc acaacccgac gagcggtaga caacaccagc gccatcagcg
     3181 tatcggcgac ggtttctgtt aatacggttg gcgtgtgcat cagcagaatt tttcgggcgg
     3241 taagcgcatc gacatcaaaa ttgtcatagc cgacggagat cgttgatgtg gcacgcagtt
     3301 tcggcatttt ttccagcaat gcggcatcta cattctcgtt tgaacccagt aaaccttcag
     3361 cttcggcaaa aattgctgca ttttgttcga cggtttgtgg gctgaggttt gccacctggt
     3421 gaacggtgaa atgctcttgc aggcgttgca gtaaatcatc aggtaaggct ttgtagagga
     3481 taacggacgg cttcatgctt ctctccattc actgataagg cgctcacctt agcctgaaat
     3541 atgatgaagg aaaactgaag aatgccaggt gatttctgca tcacctggca tgaaagggat
     3601 tacagcgggc ttaaggtaat ttctacacgg cggttttgcg ccttaccttc tgcggtgctg
     3661 ttgctggcga ttgggttagc cgggccaagg ccctgagtac ggatgcggct ggcgtccacg
     3721 ccctgggtga tcaacgcgct ggcaacggaa tccgcacgct gctgggagag acgcatgttc
     3781 aggtcgtgac cccccgtgct gtcggtataa ccaatcacgt taaccgccgt tttcggatac
     3841 tctttcagca ccattgccac gccggtcagg gtgttagcgc ctgccggttt cagggtcgcg
     3901 ctgctgctgt cgaaggtcac attgttcggc atattgagga taatgttatc cccgctgcgg
     3961 gttacgctaa caccagtgcc gcgcattttg tcacgcagct tcgcttcctg cacatccatg
     4021 taataaccca cgccgccgcc cagagctgcg cctgctgctg cgccaatcag cgcgccttta
     4081 ccgcgatctt tcttcgaaga agagagcgca ccaatacccg cgcccacgag agagtccaga
     4141 cctgcgccga tagcagattt gcctgcttcg cgttcgccgg tgtaagggtt agttgtgcag
     4201 ccagataccg ccagagcgcc actcactacg gcggcaataa gataaacacg tttcttcatt
     4261 gttaatcctt aataaccttt ttattctttg ccacgggttc cgtggcggga gattatgccg
     4321 cgtgaacatg aagattattc ctgggaatac tcggaaattt gtaagtaata tttaactgct
     4381 caatacatct aacctttcag gagtcttcgg tttggccaac tcatcctcac gatattccgt
     4441 tctgactgcc gcccactggg ggcccatgct ggttgaaacc gacggcgaaa ccgtgtttag
     4501 ctcgcgtggc gcgttagcca caggaatgga aaactccttg cagagcgcgg ttcgcgacca
     4561 ggttcacagc aatacgcggg tacgatttcc aatggtgcgc aaaggctttc tcgcgtcacc
     4621 ggaaaacccg caaggcattc gtgggcagga tgaatttgtt cacgtgagtt gggatgaggc
     4681 gctggatctt attcaccaac aacataaacg cattcgtgag gcttatggtc cggcatcgat
     4741 ttttgctggt tcctacggct ggcgttcaaa cggcgtgctg cataaggcct cgacattatt
     4801 acaacgctat atggcgctgg caggcggtta taccgggcat ctgggggatt attcgaccgg
     4861 tgcggcacag gcgatcatgc cgtatgtcgt gggtggcagc gaagtttatc aacagcaaac
     4921 cagttggccg ctggtgctgg aacatagcga tgtcgtggtg ttgtggagtg ctaacccact
     4981 caatacgctg aaaattgcgt ggaatgcatc tgatgagcag gggctttctt acttttccgc
     5041 actgcgtgac agcgggaaaa agctgatctg tattgatcca atgcgatcgg aaaccgtcga
     5101 tttctttggc gataaaatgg agtgggtggc accgcacatg ggcaccgatg ttgcgctgat
     5161 gctggggatc gcccatacgc tggtggaaaa tggttggcac gacgaagcgt ttctggcgcg
     5221 ttgcaccaca ggttatgccg tcttcgcctc ttatttgctg ggcgagagtg acggaatagc
     5281 caaaaccgcc gaatgggcgg cagggatttg tggggttcac gcagcgaaaa ttcgcgagtt
     5341 ggcggctctt ttccaccaaa acacgaccat gctgatggct ggctggggaa tgcagcgcca
     5401 acagcttggt gagcaaaaac actggatgat cgtcacgctg gcggcaatgt tggggcaaat
     5461 cggcacaccc ggcggcggtt ttggtctttc ttatcatttt gccaatggtg gtaatcccac
     5521 gcggtgttct gcggtgctct cttccatgca gggtagcttg ccgggtggca gcgatgcggt
     5581 agataaaatc cctgttgccc gtattgttga agcactggaa aaccctggct gcgcatacca
     5641 acacaatggc atgaatcgcc tcttcccgga tattcgcttt atctggtggg cgggtggcgc
     5701 caacttcacc catcatcagg acactaatcg cctgatacga gcttggcaaa aaccggagct
     5761 ggtagttatc tccgaatgct tctggacggc ggcggcaaaa cacgcggata tcgttctgcc
     5821 tgcgactacc tcttttgagc gtaatgatct caccatgacc ggcgattaca gtaatcagca
     5881 tctggtgccg atgaagcaag tggtgccgcc acgctatgaa gcgcgtaatg attttgatgt
     5941 ttttgccgag ttaagtgaac gctgggagaa gggcggttat gcacgtttta cggaaggaaa
     6001 aagtgagttg caatggctgg aaacgtttta taacgttgcc cgacagcgcg gggcaagcca
     6061 gcaggttgaa ttgccgccat ttgctgagtt ctggcaagcc aatcagttaa ttgagatgcc
     6121 ggaaaacccg gacagcgagc ggtttattcg cttcgctgat ttttgccgcg atccgcaggc
     6181 gcatccatta aaaaccgcca gcggtaagat tgaaatcttc tcgcagcgta ttgccgatta
     6241 cgcttatccg gattgcccgg ggcatccaat gtggctggag ccggacgaat ggcagggcaa
     6301 tgccgaaccg gaacagttac aggtactttc tgctcatccg gcgcatcgtc tgcacagcca
     6361 gctgaattac agttctctgc gcgaattgta tgcggtggca aatcgtgagc ctgtcaccat
     6421 tcatcctgac gatgccctgg cgcgcggcat tcaagatggc gatactgttc ggctgtggaa
     6481 cgcacgcggg caaattcttg ccggagtggt cattagcgag ggaattaaac ctggcgtgat
     6541 ttgtattcac gaaggggcat ggccggattt ggatttaacc gctgacggta tttgtaaaaa
     6601 cggcgcagtc aacgtgctga ccaaagatct ccccagctcg cggctgggga atggctgtgc
     6661 gggtaatacg gcgctggcat ggctggaaaa atacaacggt ccggaactga cacttacagc
     6721 gtttgaacca ccggccagct cataatccag gtgggtagtt gggtttcatc ctgccatgcg
     6781 caatcgacaa tgtgaaaacc ctgcgcttgg taaaaattta tcgccggttg atttttttga
     6841 taaacctcca gcatcaggtg gggatggcgc tgctgcacat attgcatcag cgccttacca
     6901 ataccgcgcc tgacggcctt cggtgcgaca aacatcgctg ccagaaatcg gccttccata
     6961 atgctgacaa aaccgagaag cttactgtct tcttcccaga cccagttttg cgcattggca
     7021 agatacgcat cccgcaccag cggaatgcaa tcacgccagt aattcgcttt tataaaggga
     7081 tgtccccagg ttgtactttc cagccacagt tcgaggatcg cggggagttc tgaacgttgc
     7141 gcttcccgaa tcatggttta tttcccggat agcaacagca gccaaccaca tgatcattca
     7201 ccagcccaca tgcctgcata aaggagtaac agattgtggt accgacaaac ttaaaaccac
     7261 gttttttcag tgctttagat agggcgtcgg aggcgggcgt agatgtgggg atttcgctca
     7321 acgttgtggc ttgtgtcacc tgtggctgat gatttacaaa cgaccagaca aagtcggcaa
     7381 acggttcgcc gttctgttcc atttgcaggt acgcccgcgc attaccaata attgcctgaa
     7441 ttttcccgcg atggcggata atcccggcgt cctgtaccag tctttcgaca tcctcttcct
     7501 gcattgctgc gaccttcacc ggatcgaact gatgaaagca ggcgcgatag ttttcgcgtt
     7561 ttttgaggac ggtgatccac gataacccag cctgctgccc ttcaaggcag atcatttcga
     7621 acagtttttt actgtcagtt tcaggcacgc cccactcatt atcatggtag gcaatataaa
     7681 gcgggtcctg actcacccag ccgcaacgtt ccatactttc cctcgctatg tacccgatcg
     7741 taaaaatttc agccgctttt cttgacgcat cggtgaaaaa gtcaatattt aatacagggc
     7801 tactatgccc gatatccttc atacaatgac aataatattg ccgaattcgg ctcttccctg
     7861 gagtcgtgtt aatgatcata aaaaaaagcg gtggtcgctg gcagctaagc ctgctggcga
     7921 gcgtggtaat cagcgccttt tttctcaaca cagcttacgc ctggcaacaa gaatatatcg
     7981 ttgatacgca acccggacat tccacagagc gttacacctg ggatagtgat catcaacctg
     8041 attacaacga tattttgtcg caacgtattc aaagtagcca aagggcgctg ggactggaag
     8101 tcaatctggc ggaagaaact cctgtggatg tgaccagcag tatgagtatg ggctggaatt
     8161 ttcctttata tgaacaggtt acaaccggcc cggtcgcggc attacattac gatggcacaa
     8221 ccacctcgat gtataacgag tttggcgaca gtactaccac gctgaccgat ccgttatggc
     8281 atgccagcgt gagtagctta ggctggcgtg ttgactcccg gcttggcgat ctccgaccct
     8341 gggcgcaaat cagctataac cagcaatttg gcgagaatat ctggaaggcg caatcaggcc
     8401 tgagccggat gacggcgaca aaccagaacg gcaactggct ggatgtcacc gtaggcgctg
     8461 atatgttgct caatcaaaat attgccgcct atgccgcgct aactcaggca gaaaatacca
     8521 ctaataatag cgactatctg tatacgatgg gggttagcgc cagattttaa cgtaacagtc
     8581 acaattgaaa ccattgaata acaatagttg tggtgatagt gggtgctaac ttacaaataa
     8641 taaatttggt gaataattgt cgcgtcattc attcctgaac taaggcattt cattccgttc
     8701 tgatggcatt tcatgccgtt tttccccagg cataaaatgc acttcgttat ggttgtcggc
     8761 agagattttt cctttttatt actgcaggaa tactgccatg acaccttcaa attatcagcg
     8821 tacccgctgg ctgacactca tcggtactat cattacccag tttgcgctgg ggtcggttta
     8881 tacctggagc ctgtttaatg gcgcgctttc tgccaagctg gatgcgccgg taagccaggt
     8941 cgctttctct ttcggcttgt taagtctggg gctggcaatt tcatcttctg ttgcggggaa
     9001 attacaggaa cgttttggcg ttaaacgcgt cactatggct tccggcattt tgctaggatt
     9061 aggcttcttc ctgacagcgc attctgacaa cctgatgatg ctgtggttaa gcgccggtgt
     9121 gctggtcggt ctggcggatg gcgcgggtta cctgctgacg ctctctaact gcgtgaagtg
     9181 gttcccggag cgtaaaggtc tgatctccgc gttcgctatc ggttcttatg gtcttggtag
     9241 cctgggtttc aaatttatcg acacgcagct gctggaaacg gtgggtctgg aaaaaacatt
     9301 tgtgatttgg ggagcgattg cgctggtgat gattgttttc ggcgcaacgt taatgaaaga
     9361 cgcaccaaaa caggaagtga aaaccagcaa tggtgtggtg gagaaagatt acacgctggc
     9421 agagtcgatg cgtaaaccgc agtactggat gttagcggta atgttcctga ctgcctgcat
     9481 gagcggcctg tacgtgattg gggtagcgaa agatatcgcc caaagtctgg cacaccttga
     9541 tgtggtttcc gcagccaatg cagtaacggt tatttccatc gctaaccttt caggtcgtct
     9601 ggtgctgggc attctgtctg acaaaattgc ccgtatccgt gttatcacca ttggtcaggt
     9661 gatttcgctg gtgggtatgg cggccctgct gtttgcacca ttaaatgcag tgacgttctt
     9721 tgcagcgatt gcctgcgtgg catttaactt tggcggcact attaccgtct ttccatcact
     9781 ggtcagtgag ttcttcggcc tcaataacct ggcgaaaaac tacggtgtga tttacctcgg
     9841 tttcggtatc ggtagcattt ttggttcgat tatcgcctca ctgtttggcg gtttctatgt
     9901 gaccttctac gtgattttcg ccctgctgat tctgtcattg gcgctttcta cgacgattcg
     9961 tcagccagag cagaaaatgt tgcgtgaggc gcatatttag aaatatgttg ctactgaaat
    10021 aaatacgagg agggagactt caaagtctcc cttttttatg gacgcatatt aaaagaacaa
    10081 taatattcat accacaaata tattttattt atatattat
//
LOCUS       DAJVUJ010000156        10002 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.156, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000156 DAJVUJ010000000
VERSION     DAJVUJ010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10002
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.156"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(267..1610)
                     /gene="gdhA"
                     /locus_tag="OQQ48_003458"
     CDS             complement(267..1610)
                     /gene="gdhA"
                     /locus_tag="OQQ48_003458"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HCS1954104.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSESEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVGAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            1846..2118
                     /locus_tag="OQQ48_003459"
     CDS             1846..2118
                     /locus_tag="OQQ48_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HCS1954105.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            complement(2084..2491)
                     /gene="nudG"
                     /locus_tag="OQQ48_003460"
     CDS             complement(2084..2491)
                     /gene="nudG"
                     /locus_tag="OQQ48_003460"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HCS1954106.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQALVRELNEELGIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPTDIPLLEAFMALRAARAAD"
     gene            2578..3189
                     /locus_tag="OQQ48_003461"
     CDS             2578..3189
                     /locus_tag="OQQ48_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCS1954107.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRELTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFISTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(3198..4505)
                     /gene="ynjE"
                     /locus_tag="OQQ48_003462"
     CDS             complement(3198..4505)
                     /gene="ynjE"
                     /locus_tag="OQQ48_003462"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HCS1954108.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPEQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(4572..5225)
                     /locus_tag="OQQ48_003463"
     CDS             complement(4572..5225)
                     /locus_tag="OQQ48_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCS1954109.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     STLKGTARRNAVKDALDRAGLAETYHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(5225..6761)
                     /locus_tag="OQQ48_003464"
                     /pseudo
     CDS             complement(5225..6761)
                     /locus_tag="OQQ48_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(6734..7900)
                     /locus_tag="OQQ48_003465"
     CDS             complement(6734..7900)
                     /locus_tag="OQQ48_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCS1954110.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVNTQLPVREDFSVPTQGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKEAPDDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPVV
                     WDDPSVLDPQKLPDGQREILQSRMPQDLPPVLAEPHAGWVNALEQEWLRRYGTH"
     gene            complement(7910..8458)
                     /locus_tag="OQQ48_003466"
     CDS             complement(7910..8458)
                     /locus_tag="OQQ48_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCS1954111.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            complement(8458..9165)
                     /locus_tag="OQQ48_003467"
     CDS             complement(8458..9165)
                     /locus_tag="OQQ48_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCS1954112.1"
                     /translation="MMKMQSRKIWYYCITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFISTGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            complement(9180..9856)
                     /gene="ydjY"
                     /locus_tag="OQQ48_003468"
                     /pseudo
     CDS             complement(9180..9856)
                     /gene="ydjY"
                     /locus_tag="OQQ48_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
     gene            complement(9861..>10002)
                     /locus_tag="OQQ48_003469"
     CDS             complement(9861..>10002)
                     /locus_tag="OQQ48_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCS1954113.1"
                     /translation="RFILQLCLAGLALFILVQLAKLYARHKHVDLSASRRSPLTHPKN
                     EG"
BASE COUNT         2386 a         2722 c         2584 g         2310 t
ORIGIN      
        1 catatctgct gctatcgcag aaagttaacg gatgctgaac ttgaagcgtg ttatcgttac
       61 gacaagcagt tttgggagca ctacagtaag aaatgccgtt ggcccataag aaatgttccc
      121 tggggagcat catccgttaa atactcataa acgcctgaaa ttttgccggg ggcgctgcgc
      181 ttgcccggcc tacattagag cgtaactgca atgaattgtg cccatttgta ggcctgataa
      241 gcgtagcgcc atcaggcatt tacaacttaa atcacaccct gcgccagcat cgcatcggca
      301 accttcacaa aaccggcaat gttcgcgccc tgcacgtagt tggtttgctc accttcacca
      361 ccatgctcaa cacaggcatg gtggatatcc agcatgatgt gatgcaaacg tgcgtcaact
      421 ttctcggctt tccagcccag gcgcgcagcg ttttgcgcca tttcaaggcc tgacgttgct
      481 acaccgccag cattagccgc tttacccggt gcaaacagta cgcctgcctg ctggaacagt
      541 tcagtcgctt cgatggtggt cggcatattt gccccttcgg cgacggcttt aacgccattg
      601 gcgataagct gatgcgcggc gccaacatcc agttcgttct gggtggcgca aggcagggcg
      661 atatcaaccg gtacagacca cggctgttgg ccttcgagat agaccagacc aaattctttg
      721 gcgtaatctg ccactcgacc atcgcggctg gctttgattt cgataagacg tgccagtttc
      781 tctttcgtga atccgctttc atcaactaca gtgccgctgg agtctgacgc agtgatcaca
      841 cgagcaccaa attccatcgc tttttcgata gcgtactggg cgacgttgcc ggagccagaa
      901 acggaaacgc gcatcccttc aaaacccata ccgtggcgtt ttaacattgc ttctgtgaaa
      961 taaaccagac cgtagccggt agcttccggg cgaataagac taccgccaaa tgaaaggccc
     1021 ttaccggtga agacgcaggc ggtattgttg gagagctttt tcatcatccc cgccataaag
     1081 ccgacttcac gaccaccaac gccgatatca cctgccggaa cgtcagtatc cgcgcccaga
     1141 tgacgataaa gctcggtcat cagcgcctgg cagaaacgca tcacttcgct ttcgctttta
     1201 cctttcgggt caaaatcgct accgccttta ccaccgccca tcggcagggt agtcagggca
     1261 tttttgaagg tttgttcgaa gccgaggaat ttgagaatgg agaggttaac tgacggatgg
     1321 aagcgcatac cgcctttgta cgggccgatg gctgagctga actgcacacg ccatgcacgg
     1381 ttgacctgta cctggttgcg atcatcaacc cataccacgc gaaactggat cacgcgctcc
     1441 ggttcaacca gacgctccag taatgacatc tggcgatatt ttggattttg ttcaagaaaa
     1501 ggccagagtg tggtcattac ttcacgaacg gcttgcgcga actcggtttg attcgggtcg
     1561 cgcttttgga catggttgag gaatgactcc agagaatatg tctgatccat agatataaaa
     1621 cccttatata ttaatacgat tgtgcttatg ttgtttatgt catgtgcttt tgcagttttc
     1681 gactatacca tcaagaatgt gacggtaagc aagaaaaaaa tgacccagga aagcaaatta
     1741 ataacgagag taatctcata accaaatgcg ataactcaga cgctaaagtt ttgccacccg
     1801 gctaccgtta tcagttgcag agacaataac gttaaggagt tccctgtgag tcgagcattg
     1861 ttcgccgtag tattagcatt tccattaatt gcactggcta acccgcatta tcgtccggac
     1921 gtggaggtaa acgtaccgcc agaagtgttc agttcaggcg ggcagtcagc gcagccctgt
     1981 actcagtgct gcgtttatca ggatcaaaat tattctgaag gggcggtaat taaagccgaa
     2041 ggaatactgc tgcaatgcca gcgggatgac aaaaccctta gcactaatcc gctgctctgg
     2101 cggcgcgtaa agccataaac gcctctaata gtggaatgtc agtaggggcc agcggatatt
     2161 gcagcgcctc ttcaggtgag caccagacca gcgcctgatg ttcatgtgcc tgtaacgtcc
     2221 cgtggaagtc gggtacgtgc caggcatgaa gatggataat ccggcccgaa acttctcgct
     2281 gatggctggc aacatactca cccacagttg cttcgatgcc caattcttcg tttaactcac
     2341 gcaccagtgc ctgctgctgg ctttcatcca gctcgacttt accaccggca aactcccata
     2401 atcccgcctg atcgctctgg gcggggcgtt gcgcgagtaa aattttgcca tcacgttcaa
     2461 tgatggcggc aacaacttca atcattttca taaattacac acgggcagat aagcgaaaaa
     2521 tgccataata tccgcactgt tgctcaatca gaaaggcttt atatttaagg agtgcctgtg
     2581 ctagaccgcc atcttcatcc ccggattaaa ccgttgttgc atcagtgcgt gcgggttctt
     2641 gataaaccgg gcattacgcc agatggttta acattagtgg gatttgccat cggcgtgctg
     2701 gctctgccgt ttctggcgct gggctggtat ctggcggcgc tagtcgtcat tttgttgaac
     2761 aggctgcttg atggtctgga cggtgcgctg gcgcggcgca gagagcttac cgacgcgggc
     2821 ggctttcttg atatttctct cgattttctc ttttacgcgc tggtgccgtt tggctttatt
     2881 cttgctgcac cggagcaaaa tgcgctggca ggcggctggt tgttgtttgc gtttatcagc
     2941 acgggcagca gttttctcgc ctttgctgca ctggcagcaa aacatcagat tgataacccc
     3001 ggttatgcac ataagtcgtt ttactatctg gggggattaa ccgaaggcac agaaacgatc
     3061 ttactgtttg tgctgggatg tttattccct gcatggtttg cgtggatctt cggtgcgttg
     3121 tgctggatga cgacctttac gcgggtgtgg agtgggtatc tgacgctgaa gtcgctccag
     3181 cgtcagtaat tgcaatgtta tttgctactg tccgggccgc gttcaccggt tgctaccgga
     3241 ttttttggat cgctgctcca ttcgtaccag ccgccgtcat acacggaaac attcttccaa
     3301 cccatggcgc gtgcgtacat aaaggtttcg gacgcgcgcc agccggtgcc gcagtagaat
     3361 gaaacttgct gctctggttt gatattccac gctttccaca tagcggtaat atcatcggcg
     3421 ctgcgcatgg tgccatccgg gttatgaaaa tcttccatat gcgtcgagtc gctaccagcg
     3481 tgtccccaac gtgctccggc tatttcacct tttggtttaa tatagctgta accgctggtc
     3541 gtaccgataa attctggcca tgaacgaatg ctcaccagcg atgcatcctg gcgatgcagc
     3601 agtccacggg cctgttccat atcgagcatc aactgcggtt gtgccgggat cttcacgccg
     3661 aaatccggtt ccgctttcac tttcggtggc gttcctcgct caacaggcag tcccgcgtcg
     3721 gaccaggttt gccagccgcc atccagcaga cgcacatctt tcacgcctgc gtagagcata
     3781 atttgcgcca cacgcgctcc agcgtatacg tcacgcccgt acagaatgac cgtggtgtca
     3841 tggcgaatgc cgtgttttgc cagcatcgct tttagttgtt catcagaaac tttgttccac
     3901 agcggttcgc tttccacttc gttggtatcg atgtagtcag caccgggaat atggctgata
     3961 aggtaaagct taggagcgcc ccaggccgct tcaatgactt tccagtcacc ggcaggtttc
     4021 gccgtaacct ctttaccttg ttgcaggtcg tgcagccatt gcggataaac cagctgctca
     4081 aagtgcggca gtttttgtag acgggaaggt tcgcttagcg cgtcactcag aatggagata
     4141 tgcgtaaaac ctgctttttg cagtcgcgtt ttgacggcgt cgacatcttt gtcattaccg
     4201 tacagcgcca ccggggcatc ggctttcagg ttatgttgct tgatccacgc gttgagctgt
     4261 tcggtgctca ttttgtcgag ccagctggca gagaggttta aggcggcagg ttcatgacca
     4321 gaagggccat ttaaggtttg tggccagccg ttataaaacg cgctggggcg agtatctatc
     4381 gctttgccat tttgttgttg aagctggtca agtgtaagag gcttcgccag ttcagcggcc
     4441 cacgaagaag cgcaagccag ccctaaagcc attgccagcg cggtcatttg agaaacacgt
     4501 ttcatcgaaa atcccgacat tgtagttttg agtgcgccat tctgaactga cgcattgaga
     4561 aaaactttgc gttatcgcag tttgttgtaa ttttctgacc actgcgccat atccagaaca
     4621 gaactatcag caggaacatc ctggagatcg tgcgttacct gaacgacggg gatcgccagt
     4681 tcgcgaactt cgctgaacac ccactggcga aaattatcgc gcagagccac atcaagacgg
     4741 ctgaatggct catccaggag taacgctttt ggctgagcga gaagggcgcg cagcagggcc
     4801 acccgcgctc gctgaccgcc tgataatgtc gcagggtcct ggtgataagt ttcagccagt
     4861 cccgctcgat caagcgcgtc tttcacggca ttgcgcctgg cagtcccctt gagcgtcgac
     4921 ggtagcgcca gcagtaaatt ttgcccgacg ctgaactggt caaataacag tgcatcctgg
     4981 aaaagaatgc caatctgacg ctgtgcggtg ggtagcatgt caatccgttg ctcattgagc
     5041 catagctcac ctgtacaaga aaactgtcca gccagtgcac caatcatcca tgaaaacaga
     5101 gtggattttc cacagccaga cggccccatt aacgtgacga tgtcaccttt atcaaccgta
     5161 aagttaacgt ttgtcagcaa gcggctttct ggtaaacgta gcgaaacatt tttcacgcag
     5221 agcattagcg gagtccttgt ctgacataac ctacccattt tgcgactaac gcggtcaggg
     5281 caaaaataat aagcggtaac aacagttgcc ataaagcctg ggcggcgaga ataccgttgc
     5341 tgccgccgct gcttaatgcc accgcctcag tggtgagcgt cggaaaacgc cccgcgccca
     5401 gccacagcgt cggcatatac tgcgcaatac tgactgaaaa tcccaccgcg aaggcaatca
     5461 gcacagggcg caacatgagt gtgcatttca cgtaaaagaa gattttggcc cgctaccagc
     5521 ccagtgtttg tgcaatcaaa attaaccgtg aatcaatgcg ctgccaggcg ggttgcagga
     5581 taaacagcat ccacggcatt acccacagca gatgccccca gaccaccgcc gtccagcttc
     5641 catccaggtt cagccatagc gccagcgtgt actggcccgc caccagtggc agagcgggca
     5701 atagaatggg caaccatagc cacaactggc gacgctgtgg cccccattcc agccacagca
     5761 gtaacaggag caaagcgatg aatgttgcta ccagccccat tgtcagactg ttgatgagtg
     5821 cttcactatt gatcgtcgac tgatccgcga ggatcgccag cagaaccaca cacagcacac
     5881 cggttaaggg taaaaaactc gccagcgtag tgcctggtaa taaaggtgtg gcaggcattg
     5941 cgaatgccat ctacgcgggg aatagtgcgc cgccagctgc gccatagcag atagctcagc
     6001 aaaacgtagg cggcgagtaa cagcatcagc agcagactgg cgagtgcgcc tttcgtttgt
     6061 tgatccgcgt cgccctgagt taaccactgc cagctaatta ctgccagcgt cggtggatta
     6121 cctggcccaa gaataatcgc cacatccacg accgaaagcg accaggcaac aatcgccagc
     6181 atcgccattg ccagcgaagg cgcgacggag ggcaacagca gccagttcag gcattgccag
     6241 cggctgtagc ccagcgaatc cagcacaatg acctgctgca acagccgttt ttcgctcaat
     6301 actgccgcta agatccacag cagaaatgcg ctttctttca ctgcaagggt gaggcccaga
     6361 ccgatgccaa atcggtccat tggcggagtg aaatacggga aatagtcata tagcagccct
     6421 ccgtcagcaa agagcagcag ggcgctggtg gcaaaagcca catggggaat ggcgagcagc
     6481 cacggcagac gggcgcacat acgctgccat ttcggcccag gccatagcgc cacaatcacc
     6541 agcagggcaa tcaacaatgc cccgaccgcc gcgatggttg tcgacaccag cgttgccagt
     6601 aatgcatgcg gtaactgtgg atcggcaaat aacgcctgcc agtgtgtcaa cgacaacgca
     6661 ggcgatatca gcgtgagagc tgccgggatc agcggtgcat aaatcaccgc caccatcgcc
     6721 cacagcaaaa agattaatgc gtaccgtaac ggcgtaacca ttcttgttcc agtgcattca
     6781 cccatcctgc gtgcggttca gccagtaccg gcggcagatc ctgcggcatt cttgattgca
     6841 atatttcgcg ctgcccgtca ggcagttttt gcggatcgag aacagaagga tcgtcccaga
     6901 caacgggatc tgctttacgc agttgcgcat cgggtgagag cagaaaattg gcaactacct
     6961 tcgccgcagc actggcattc gcgttggctg gaatggtgac gaaatgcacg ttgccaatca
     7021 tcccctcgcg aaagccaaaa ctgtaactgc ttgccggcaa atcgccgctg gcgatttttt
     7081 gctgcgcatg cgcggggtta aaggtcagcg acaggcgcaa tgtgccggct ttcagcagag
     7141 catccatccg cgcgggcgaa ggcgggaaat cctttccttc gcgccacaaa tacggatgca
     7201 gtgcatcaag atattgccac aagggcgcag tgacacgggc gaaagtcgca tcgtccggcg
     7261 cttcttttaa tgcggcggga tctggcgtca gcataatcag caactgttca agaaacgccg
     7321 tgccggtaaa gtccggtggg cgcggataag taaccgtgcc gggattggct ttagcaaact
     7381 ccagtaaggc ttgcggcgtt tgtggtggct gtggcgtaac atcgcggcgg gcgataaacg
     7441 tcagttgtgc gccgccccag ggcgattccg caccttgtgt cggcactgaa aaatcttccc
     7501 gcaccggcag ctgtgtgttg acatagcgcc agttgggcag agtctccgcc cagcccgttt
     7561 gtaataaatt ggcctctttt aaggtgcgga agttttcgcc gttcacccag agcagatcca
     7621 ccgagccgcc cgttttacga ccggcagcag cttcggtctg aatgcgcttc accgcgtctg
     7681 cggcatccgc gagacgaaca atcttcaggt ttatagcgta atgggttttc atctcgccgc
     7741 taacccagtc gagatagcgg ttaattgcgg tatcgccgcc ccaggcgtta aaccagacgg
     7801 tttgcccttt ggcctcattt ttaatttcct gccagtcact ggcatgtgtt gccaaagaaa
     7861 acagcgataa caatcccagc aaccacccac aatggcgcat cttctctcct taagaacgtt
     7921 tttccggaat acggcacagc ccctgagcgg gaatgtccat ggcagaatta aaacgagccg
     7981 acaggttttg caggccgatc aatgccgtca gttcggtgag cgcctgagcg tcaaaatgcg
     8041 cagccagtcg ggtacgcagg gcatcatcga ctgttggcgg tgtgacgctt gcggcttcgg
     8101 cgtactccag cgccagccgt tcttcatcgc taaagagcgg gctttgccgc cagtcagcca
     8161 ctgccagcag tttatcggtg ctgccggtac gctcggcgac tttcatactg gtgatatcca
     8221 cacaaaactc acacaggcac atttgcgcaa tgcgcgcgct gacaagcgat cgcactaccg
     8281 gatcgagcgg tgagcgtttg cgctccagcc acccaacaaa cattgacacc aaataaaaga
     8341 taaacgggat ccgcccccac cagcgaatcg ggcttagcac ctcgccgtaa tggcgacgat
     8401 gcagccacgc ctgcggacgt aaaatgaaag gaattttgct aagcggcggt aaacccatca
     8461 gtcattcctc ttctggcgct caagccatat tttcttcgcc ataaaaatca ccacggtcag
     8521 ggcaaacaga ataaacagtc cggtgacaaa ccagaaagta ccgcctgtta acatgctgcc
     8581 cgcccaggaa taaacaatag tcgccggtaa ttgaccaagc ccggtggaga taaaaaacga
     8641 gcgaaaacgt attgaagtca gaccggcagc atagctgatt ggatcgaaag ggacaaaagg
     8701 caataaccga cagaccagga tggtgtgttt gccgtagcga gtgaaaaagc cgtccatact
     8761 gtcaagcacg gttttgccgg ttaatttttc caccacttcg cggcccatca ctctggcgat
     8821 aaaaaagcac agcgccgcgc cggccatcga actggtccac gacagcaagc cgccccagaa
     8881 cgcgccaaac agcgacgcat tggcaaaggt gatcaaaaac gcaggtagcg gtgcagcaat
     8941 ggcctgtaaa atcatcaaca agaacgagac aactgccgcc agtgcgccgt aagactgaat
     9001 aaagcgttct ataccctgtt ggtccacggc ggcaaacgcc gcaacgctgc gattgataaa
     9061 ctcatggacg ccgggaagca gcgcccatgc gagcagcata gcgaacaaca ggatgatgag
     9121 ggttatacag tagtaccaga ttttacgcga ttgcatcttc atcatccttt atcctggctt
     9181 tattcggtga ttttaaaggt aaccgttgcc agcgtgttat ccgccgggag aactgaggca
     9241 ttgcctttga atttcacttc gccgcgtttt tccaccgcac cataagtgta tgttgcattg
     9301 ctgacgatgc cgaccgggca gctatccaga cacaccagtc aacccgtttt cttctcttct
     9361 gctgccgtta aattaccgcc aaagcgcatg tccagtttct tgccattact gtcaacaatc
     9421 acttcatcga aggaatacgc ttttgccgcc ccttgccagt tgaccgaaat atccagtttg
     9481 ctgcctgtga catgagtcgt ttctttatta tccatcgtca tgttttcgcc cggcgtacca
     9541 cctgctcttt cagggcttca taaaatgctt tcggggtcgc ataagccata aacagcgatt
     9601 tatgtccgtt ggagccatct ttaaacacaa taccgtgacg agtgtcgtcg gtgaggaaac
     9661 gaccattaat ttgcaccagc atggttaccg tatggttatt ggcatcgacg cgcagcggtt
     9721 ggctgttcga aagtccgtcg tattccactt ttgccgccga attctctttt tgatcgcagc
     9781 cgctaagccc ggcaacagcc agtaagcaga gtgcagccag tccttttttc catgacactg
     9841 aataatgttg cgacatcgtt ctatccttca ttttttgggt gagtaagcgg gctgcggcgc
     9901 gaagcagaca gatccacatg tttgtgtcgg gcgtagagtt ttgcgagctg gacgagaata
     9961 aacagcgcca ggcccgccag acagagttgt aaaataaagc gc
//
LOCUS       DAJVUJ010000157         9957 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.157, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000157 DAJVUJ010000000
VERSION     DAJVUJ010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9957
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.157"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            259..456
                     /locus_tag="OQQ48_003470"
     CDS             259..456
                     /locus_tag="OQQ48_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1954114.1"
                     /translation="MEITPQQLVEFTIPDGRGQQARTLPDMLSSDAGPLSYPLIYSSK
                     VMIISLFDSTVMPDKYRHNGK"
     gene            complement(406..1599)
                     /locus_tag="OQQ48_003471"
     CDS             complement(406..1599)
                     /locus_tag="OQQ48_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1954115.1"
                     /translation="MSPGIEDTPQKTLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFVTDTTAISQQVAWLLGLSAVAALIAQFGV
                     VRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            1735..3459
                     /gene="iucA"
                     /locus_tag="OQQ48_003472"
     CDS             1735..3459
                     /gene="iucA"
                     /locus_tag="OQQ48_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HCS1954116.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3460..4407
                     /gene="iucB"
                     /locus_tag="OQQ48_003473"
     CDS             3460..4407
                     /gene="iucB"
                     /locus_tag="OQQ48_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HCS1954117.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4407..6149
                     /gene="iucC"
                     /locus_tag="OQQ48_003474"
     CDS             4407..6149
                     /gene="iucC"
                     /locus_tag="OQQ48_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HCS1954118.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFVEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDATSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6146..7483
                     /gene="iucD"
                     /locus_tag="OQQ48_003475"
     CDS             6146..7483
                     /gene="iucD"
                     /locus_tag="OQQ48_003475"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HCS1954119.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFARNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVITLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            7489..9684
                     /gene="iutA"
                     /locus_tag="OQQ48_003476"
     CDS             7489..9684
                     /gene="iutA"
                     /locus_tag="OQQ48_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HCS1954120.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLSLTKSVNVSDSKLEGVKVDSYELGWRFTGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
BASE COUNT         2395 a         2565 c         2646 g         2351 t
ORIGIN      
        1 cataaaaaac atgtcagtga aaaagaaacc tggcgttggg ttgaaggata taataaagca
       61 acagaactgg ctcgccaatg ccctgatacc catgtattgt caatttctga tcgtgaaggc
      121 gatttttatg acctgtttga acgcgcagag cagactcccg gaataaaagc tgactggctt
      181 gtcagaatga aatttaataa cagagcaacg ctgaatatta atggtaaacg ggatcatagg
      241 ttgttgcacg aaagaatcat ggagattaca ccgcagcaac tggttgaatt cactatacct
      301 gatgggcggg ggcagcaagc cagaacactg cctgacatgc tttcatcaga cgcaggtcct
      361 ctctcatatc ctctgattta ctccagtaag gtgatgatca tatctttatt tgatagtacg
      421 gtaatgccag ataaatacag acacaatggt aaataatata gttgcaaata atgctgccat
      481 aatcagcgcg attgcgacac ctgttttcga taccagcggc accagcaaag cgcacagtcc
      541 atagccaagt gtgtgactgg tagcgagcca gcccgcgcct gcgccatcag ccagcttatc
      601 attcagtaga agttgataag cgggtgttgc cagagctgct ccaaatgaca gcactgcaca
      661 gcccgggtaa aacaaccata gctgttcgga gagcatgata gccagaccac cactcatcaa
      721 cactccggca ctcaggagca gggccaccgg agtcaggcgc tgcggacgga ctaccccgaa
      781 ctgcgcgata agcgcagcta ctgcggacag acccaacagc caggccacct gttggctaat
      841 ggcggtggta tccgtgacga actggcgagt aagggcaggc gaaagtccaa gctgcatcat
      901 gctcaccgcc gcagccagta atattgcgca aagcagataa ggcagacaat cccgcttcag
      961 actgacgctc ttgcactccg gtgtgggctg tggtggtgtg ccgggcaacc gcagcagcat
     1021 cagcagcgcc agcactggtg ctgccatcag tagccccagt ggcgccagag gatgaatggc
     1081 cagcattgct gccgcgcaca gcggcccgaa tagcctgccg caactcaggc cggagctgat
     1141 ggttgccaga gcggccatgc gattcccttc tccagctctc tgcaatgccc agacctgaca
     1201 ggctggcacc attgccgaca cggtcagccc gtaggcgatc cgggcgatga ttaatattcc
     1261 cagtcccacc gctgttgtta tggcgctggt agccatcagc acgcttccca gccctagcag
     1321 ggtaaagctt atgccatatc ctcccagagc ccacaacacc acaggtttac tgccagtacg
     1381 ggaaatttgc tttccccacc acggggacga tggcagaaac agcattgagc caagcatcag
     1441 cagggcagcc catacagaca gactcagatt tgtctgtatg accagaacag ggagcacaac
     1501 cagcagaccg ttctgtccga taccgagaag cccggcactg aacgcaagtg gccagcagga
     1561 cagtgttttt tggggcgtat cttcgatccc tggtgacatt ataatgcttc aactccatgt
     1621 attaattgtg tttatttgta aaattaattt atctgacaat aacatttatt gttgataatg
     1681 agaatcatta tcgacataat tgttattatt ttactgtgtg ggagctgttt gactatgacc
     1741 ctgccctctg aaaaaccagc cacagatgtg gctgcgcagt gcttcctgaa tgcactgatt
     1801 cgtgaaacca cagactggaa actgacagaa tacccgccag acgaattgct catcccgctg
     1861 gatgagcaga aatcgctcca tttcagagtg gcttatttct ccccaaccca gcatcaccgc
     1921 tttgcatttc ctgcacgtct ggtcacggca tcaggcagtt atcctgtcga ctttaccact
     1981 ctctcccggc ttattattga taagctacgc catcaactgt ttctgcccgt tcccctctgc
     2041 gaaactttcc accagcgcgt gctggaaagc catgtccata cgcaacaggc aattgatgcc
     2101 cgtcatgact gggccgctct gcgtgaaaaa gcgttgaatt ttggcgaggc tgagcaggcg
     2161 ctgctgacag gacacgcttt ccaccctgcg cctaagtctc atgaaccgtt taaccggcgg
     2221 gaggctgaac gctacctgcc tgatatggca ccccacttcc cactgcggtg gttttcggtg
     2281 gataaaacgc aaatcgccgg tgaaagtttg catctcaacc ttcaacagcg gctgacgcga
     2341 tttgccgcag agaatgcgcc tcagttactc aacgaattaa gtgacaacca atggctgttc
     2401 ccgttgcacc cgtggcaggg ggaatatctt ttgcagcagg ggtggtgcca ggcacttgtt
     2461 gctaaagggc tgattaaaga cttaggtgag gccggcacgt cgtggctgcc gaccacctct
     2521 tcccgttccc tctactgtgc caccagccgc gatatgatca agttctccct gagcgtacgg
     2581 ctgaccaact ccatccgtac tctgtccgtg aaagaagtga agcgtgggat gcgcctggca
     2641 cgcctggctc aaaccgacgg ctggcagatg ctacaggtcc gcttcccgac tttccgggta
     2701 atgcaggagg atggctgggc cgggctgctc gatcttaacg gcaacatcat gcaggaaagt
     2761 ctgtttgccc tgcgtgaaaa tctgctggtg gatcagccga aaagccagac taacgtactg
     2821 gtctccctga ctcaggccgc acctgatggc ggtgattcgc tgctggtttc ggcggtaaaa
     2881 cgcctgagcg atcgcctcgg tatcactgtg caacaggccg cccatgcatg ggtcgatgca
     2941 tactgtcagc aagtgctaaa gccgctgttt acggctgaag cggattacgg cctggtgctg
     3001 ctggcgcatc agcaaaatat tcttgtccag atgcttgggg atctgccggt cggatttatt
     3061 taccgtgact gtcagggcag cgcttttatg cctcacgcga cagactggct cgattctatt
     3121 ggcgaggcgc aggcggaaaa tattttcacc catgagcagt tgctgcgcta tttcccttat
     3181 tacctgctgg ttaactccac ttttgctgtg accgctgcgc tgggggctgc cgggctggac
     3241 agcgaatcga atctgatggc tcgtgtacga gcatcgctgg ctgaagtgcg tgatcaggtg
     3301 actcataaaa cctgtctcaa ctacgtgctg gaaagtccgt actggaacgt aaaaggtaac
     3361 tttttctgtt atctgaacga tcataacgag aacaccattg ttgacccttc ggtgatctat
     3421 ttcgatttcg ctaacccgct gcaggctcag gaggtctgaa tgtctgaggc aaacattatt
     3481 cacagcagat atggactgcg ctgtgaaaaa ctcgacaagc ccctgaatct tggctgggga
     3541 ctggacaata gcgcggtgtt gcactgtccc ggggagctgc cgacagggtg gctgtgcgat
     3601 gcgctggatc agatatttat cgccgcacca caactgtcag cagtggctct cccctgggcc
     3661 gaatggcgtg aggagccaca ggcgctgacg ctttttgggc aggtaaaaag cgacattatc
     3721 catcgtactg ccttctggca gttaccgtta tggttgagtt caccggcaaa ccgggcctcc
     3781 ggtgaaatgg tttttgatgc agagcgtgag atttatttcc cgcagcgccc accccgtcca
     3841 cagggtgagg tttaccgccg ttacgatcca cgtattcgca ggatgctgag tttccgcatt
     3901 gccgatcccg tttctgatgc agaacgtttc acccgctgga tgaatgatcc gcgcgttgag
     3961 tatttctggg agcaaagtgg ctcactggag gtacagaccg cctatctgga gcgccagctc
     4021 accggtaaac atgcattccc gctgattggg tgcttcgacg atcggccatt cagctatttc
     4081 gaaatctact gggcggcgga agaccgcatt ggtcgtcact attcgtggca gccctttgac
     4141 cgtggcctgc atctgctggt tggtgaacag caatggcgcg gggcccacta tgtgcaaagc
     4201 tggctgcgcg ggctgacaca ttacctgctg ctggatgagc cccgtacgca gcgcacagta
     4261 ctggagccac gcaccgataa ccagcgcctg ttccgccatc ttgagcctgc gggataccgg
     4321 acaattaaag agttcgactt cccacataag cgctcgcgca tggtgatggc ggatcgtcat
     4381 cacttcttca cggaggtcgg tctgtaatga atcacaagga ctgggatttt gttaaccgtc
     4441 ggctggtggc aaaaatgttg tctgagatgg agtatgagca ggttttccac gcggaatctc
     4501 agggcgatga ccactactgc attaacctgc cgggagcaca atggcgcttc atcgctgaac
     4561 gtggtatctg gggctggctg tggattgatg cccaaacgtt gcgctgcacg gacgaaccag
     4621 tactggcgca gacgctgctg atgcagctaa agccggtgct gtcaatgagt gatgccactg
     4681 ttgcagagca tatgcaggat ttgtatgcca cgctactggg cgacctgcaa ctgctgaagg
     4741 cccgtcgcgg actgagtgcc agtgacctga ttaatcttga tgccgatcgt ctgcaatgcc
     4801 tgctgagcgg tcatcctaaa ttcgttttta ataaaggtcg ccgtggctgg ggcaaagagg
     4861 cgctggaacg atatgcgcca gagtatacca acaccttcag actgcactgg ctggcggtaa
     4921 aacgtgaaca tatgatctgg cgctgcgata acgatctgga tattcagcag ttgttgactg
     4981 ccgcaatgga tccgcaggag tttacacgct tcagtcaggt ctggcaggaa aacgggctgg
     5041 atcacaactg gctaccgctg ccggtacatc cgtggcagtg gcagcaaaaa atcgccacag
     5101 acttcatcgc tgattttgtc gaaggcagga tggtgtctct cggcgagttt ggcgaccagt
     5161 ggctcgccca gcagtcgctg cgtaccctga ccaacgccag ccgccgggga gggctggata
     5221 tcaagctgcc gctgaccatc tacaacacct catgctaccg ggggatccct ggcagatata
     5281 tcgctgccgg accactggct tcacgctggc tgcaacaggt ttttgcgacc gacgccaccc
     5341 tggtgcaaag cggcgcagtg atccttggtg aaccggcggc aggctatgtg tcccatgaag
     5401 gctatgccgc gcttgcccgg gctccctatc gctaccagga aatgcttggt gttatctggc
     5461 gggagaatcc gtgccgctgg ctgaaaccgg atgaaagtcc tgttctgatg gcaacactga
     5521 tggagtgcga cgaaaacaat cagccgctgg caggcgcata tatcgaccgc tccggactgg
     5581 acgctgaaac ctggcttacg caattgttcc gggtggttgt ggttccgctg tatcacctgc
     5641 tttgccgcta cggtgtcgcg cttattgcac acggacaaaa tataacgctc gccatgaaag
     5701 agggggtacc acagcgtgtt ctgctgaaag acttccaggg cgatatgcgg ctggtgaaag
     5761 aagcgtttcc cgaaatggat tctttgcctc aggaggtgcg tgatgccaca tcccgtctga
     5821 gtgcggacta cttaatccat gatttgcaga cgggtcactt cgtgacagta ctgcgtttta
     5881 tttcgccact gatggttcgt cttggcgtac ctgaaaggcg attttatcaa ctgctggcag
     5941 cagtgttgag tgattacatg aacaaacatc cacaaatggc agagcgtttt gcgcttttct
     6001 cactcttcag gccacaaatc attcgcgtgg tgctgaaccc ggtaaaactg acctggccgg
     6061 acctggatgg cggcagtcgc atgctgccga attaccttga gaatctgcaa aatccgctgt
     6121 ggctggtaac tcaggaatat gaatcatgaa aaaaagtgtc gattttattg gtgtcgggac
     6181 agggccattt aatctcagca ttgctgcatt gtcacatcag atcgaagaac tgaactgtct
     6241 cttctttgac gaacatcctc atttttcctg gcatccgggt atgctggtac cggattgtca
     6301 tatgcagacc gtctttctga aagatctggt cagtgcagtt gcacctacaa atccctacag
     6361 ctttgttaac tatctggtga agcacaaaaa gttctatcgc ttccttacaa gcaggctacg
     6421 tacagtatcc cgtgaagagt tttctgacta tctccgctgg gctgctgaag atatgaataa
     6481 cctgtatttc agtcataccg ttgaaaatat tgatttcgac aaaaaaagtc gattgtttct
     6541 ggttcagacc agtcggggag aatattttgc ccgcaatatc tgccttggta caggaaaaca
     6601 accttattta ccaccctgtg tgaagcatgt gacacaatcc tgtttccatg ccagtgaaat
     6661 gaatcttcgt cggccggacc tgagtggaaa acggataacc gtggttggtg gaggacagag
     6721 tggtgcagac ctgttcctta atgcattacg cggggaatgg ggagaagcgg cggaaataaa
     6781 ctgggtctca cggcgtaata attttaacgc actggatgag gctgcttttg ctgatgagta
     6841 ttttacacct gaatatattt caggcttctc cggactgaag gaagatattc gccatcagtt
     6901 actggatgag cagaaaatga catcggatgg catcactgcc gattctttac tgaccattta
     6961 tcgtgagttg taccaccgtt ttgaagttct gagaaaacca agaaatatcc gtctgctacc
     7021 cagtcgctcg gtaataactc tggaaagtag tggtccgggc tggaggttat tgatggaaca
     7081 tcatctggat cagggcagag agagcctgga aagtgatgtg gtgattttcg ccacaggtta
     7141 ccgttctgca ttgccacaaa tacttccctc actgatgccc ttgatcacca tgcacgataa
     7201 gaacaccttt aaagtgcgtg atgacttcac tctggaatgg agtggcccga aagaaaataa
     7261 catctttgcg gtcaacgcca gcatgcaaac tcatggcatc gccgaacccc agctcagcct
     7321 gatggcctgg cgatctgcac gtattcttaa tcgcgtactg gggcgtgatt tattcgatct
     7381 cagtatgccg cccgcactga ttcagtggcg cagcggcagc cggaaaaaac cgcagccgga
     7441 ggctgcttct ttaactcact atacagcaaa tattcaggaa taacgatgat gcgcaaaaag
     7501 tatatgcccc gggctcttgg tccgctgctt cttgtcgtgc tgtcaccagc tgtcgcccag
     7561 caaaacgatg ataatgagat catagtgtct gccagccgca gcaatcgaac tgtagcggag
     7621 atggcgcaaa ccacttgggt tatcgaaaat gccgaactgg agcagcagat tcagggcggt
     7681 aaagagctga aagacgcact ggctcagtta atccccggcc ttgatgtcag cagccagagt
     7741 cgaaccaact acggtatgaa catgcgtggc cgcccgctgg ttgtcctgat tgacggtgtg
     7801 cgcctcaact cttcacgttc cgacagccga caactggact ctgtcgatcc ttttaatatc
     7861 gaccatattg aagtgatctc cggcgcgacg gccctgtacg gtggcgggag taccggaggg
     7921 ttgatcaaca tcgtgaccaa aaaaggtcag ccggaaacca tgatggagtt tgaggctggc
     7981 acaaaaagtg gctttaacag cagtaaagat cacgatgagc gcattgccgg tgctgtctcc
     8041 ggcggaaatg accatatctc cggacgtctt tccgtggcat atcagaaatt tggcggctgg
     8101 tttgacggta acggcgatgc caccctgctt gataacaccc agaccggcct gcagcactcc
     8161 aatcggctgg acatcatggg aaccggtacg ctgaacatcg atgaatcccg gcagcttcaa
     8221 ctgataacgc agtactataa aagtcaggga gacgacaatt acgggcttaa tctcgggaaa
     8281 ggcttttccg ccatcagcgg gagcagcaca ccatacgtca gtaaggggct gaattctgac
     8341 cgcattcccg gcactgagcg gcatttgatc agcctgcagt actctgacag tgatttcctg
     8401 ggacaggaac tggtcggtca ggtttactac cgcgatgagt cgttgcggta ctacccgttc
     8461 ccgacggtaa atgcgaataa acaggcgacg gctttctcct cgtcacagca ggataccgac
     8521 cagtacggca tgaaactgac tctgaacagc caactgatgg acggctggca aatcacctgg
     8581 gggctggatg ctgagcatga gcgctttacc tccaaccaga tgttcttcga tctggctcag
     8641 gcaagtgctt ccggagggct gaacaaccat aagatttaca ccaccgggcg ctatccgtca
     8701 tatgacatca ccaatctggc ggccttcctg caatccagct atgacattaa tgatattttt
     8761 accgttagcg gtggcgtacg ctatcagtat actgagaaca gggtagatga tttcatcgac
     8821 tacacgcagc aacagaagat tgctgccggg aaggcgatat ctgccgacgc cattcctggt
     8881 ggttcggtag attacgataa ctttctgttc aatgctggtc tgctgatgca catcaccgaa
     8941 cgtcagcagg catggttcaa tttttcccag ggggtggcat tgccggatcc ggggaaatat
     9001 tatggtcgcg gcatctatgg tgcagcagtg aacggccatc tttccctgac aaagagcgtg
     9061 aacgtcagcg acagtaagct ggaaggcgtg aaagtcgatt cttatgaact gggctggcgc
     9121 tttaccggtg acaacctgcg gactcaaatc gcggcatatt actcgctttc caataagagc
     9181 gtggaaagga ataaagatct gaccatcagt gtgaaggacg acaggcgccg tatttacggc
     9241 gtggaaggtg cggtggacta cctgatcccg gatactgact ggagtaccgg tgtgaacttc
     9301 aatgtgctga aaaccgagtc gaaagtgaac ggtcaatggc aaaaatatga cgtgaaggaa
     9361 tcaagtccat cgaaagcgac agcttacatt aactgggcgc cggaaccgtg gagtctgcgt
     9421 gtacagagca ccacttcttt cgacgtaagc gatgcagagg gtaacgatat taatggttac
     9481 actaccgtcg attttatcag tagttggcag cttccggtgg gaacactcag cttcagcgtt
     9541 gagaacctct tcgaccgtga ctataccact gtctggggac agcgtgcacc tctgtactac
     9601 agcccgggtt acggccctgc ttcactgtac gactacaaag gccggggccg aacctttggt
     9661 ctgaactact cagtgctgtt ctgacgggga tgcttttgca acaagggtat gctgtaaaca
     9721 acatggtcct tggtggccat gttgtttaca gccagtataa tatacagcct taacaagata
     9781 ttccgtctcc ggaatggact gttcaccact gaataacatt atttctgttt tatggatctt
     9841 tgactgaaat ttatcgcata tcctcatttc attgtccgtc tgaaaactaa ccttacaagt
     9901 aagcgtcgtc tcagcaccgt ctggcagatc ctgaaattcc tgagaggatg atctgcg
//
LOCUS       DAJVUJ010000158         9887 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.158, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000158 DAJVUJ010000000
VERSION     DAJVUJ010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9887
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.158"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(46..1329)
                     /gene="aroA"
                     /locus_tag="OQQ48_003477"
     CDS             complement(46..1329)
                     /gene="aroA"
                     /locus_tag="OQQ48_003477"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCS1954121.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(1400..2488)
                     /gene="serC"
                     /locus_tag="OQQ48_003478"
     CDS             complement(1400..2488)
                     /gene="serC"
                     /locus_tag="OQQ48_003478"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HCS1954122.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSIFNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(2687..3379)
                     /locus_tag="OQQ48_003479"
     CDS             complement(2687..3379)
                     /locus_tag="OQQ48_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HCS1954123.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            3509..5269
                     /locus_tag="OQQ48_003480"
     CDS             3509..5269
                     /locus_tag="OQQ48_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HCS1954124.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            5675..6532
                     /gene="focA"
                     /locus_tag="OQQ48_003481"
     CDS             5675..6532
                     /gene="focA"
                     /locus_tag="OQQ48_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HCS1954125.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            6587..8869
                     /gene="pflB"
                     /locus_tag="OQQ48_003482"
     CDS             6587..8869
                     /gene="pflB"
                     /locus_tag="OQQ48_003482"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCS1954126.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            9061..9801
                     /gene="pflA"
                     /locus_tag="OQQ48_003483"
     CDS             9061..9801
                     /gene="pflA"
                     /locus_tag="OQQ48_003483"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HCS1954127.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
BASE COUNT         2533 a         2486 c         2352 g         2516 t
ORIGIN      
        1 cgttttgata aagaaagatt tggctattta ttgcccgttg ttcattcagg ctgcctggct
       61 aatccgcgcc aactgctcga aataatccgg aaatgttttg gccgtacatt tgggatcaag
      121 aatcgtcact ggtgtatctg acaacgccac cagcgagaaa cacatcgcca tccggtgatc
      181 attgtatgtc gcgatctcgg caaagttcag tttttccggt ggagtgatac gaatgtaatc
      241 gtgcccctct tctacttccg caccgacttt acgcagttct gttgccatcg caaacaggcg
      301 atcggtttct ttaacacgcc agttatagat attgcgcagc gtggtggtgc cttttgcaaa
      361 taacgccgcc gtggcaatgg tcatcgccgc atcgggaata tggttcatat ccatatcaat
      421 agcgttcagt tcaccacgcg tgcaggaaat ataatcatcg ccccagcaaa tggtcgcgcc
      481 cattttttcc agcacatcag caaagcgaat atcaccctgc atactgttac gtccaatacc
      541 ggtcactttt acagtgccgc ctttgattgc tgctgctgcc agaaagtaag aagccgaaga
      601 tgcatcgcct tcgaccaaat aagtacccgg agactgataa gactgtccgc cttttacgac
      661 aaattgttga tagtgctgat tttcaatttc aacaccaaac gtcttcatca gattgagtgt
      721 gatgtcgata taaggtttag aaaccagatc gcctttaata cgaatcaccg tatcttccgg
      781 cgcaagaggc gcagtcatta acagtgcggt gaggaattgg ctggaaacgg agccatcaac
      841 gtcaacgttg ccgccagtaa agccgccctg taaacgcaac ggcggataat tttcttgttc
      901 caggtaagtg atcttcgccc cgcccaggcg cagcgcatcc accagatgac caatcgggcg
      961 ttctttcata cgcggctcac cggtcagcac aatatcattg ctacccagac aaagagctgc
     1021 cgccagcgga cgcattgccg ttccggcgtt accgaggaac aactccaggg caccttctgc
     1081 gtgtaatgga ccgccgttac cgataatttc gcaacgcgta cgatcggctg aaagcgtata
     1141 gcttacccct aacgctgtta atgcattcag catatggcgc acgtcatcgc tatccagcag
     1201 attggttaat actgttttgc cgtgtgctaa tgccgccagc aataaagcgc ggttagaaac
     1261 gctcttggaa ccgggcagat taatagtgcc atcgacacga gcgatgggtt gtaacgtcag
     1321 ggattccatg aactcaactc tctacaacag aaataaaaac cccacaggct ggctgtgggg
     1381 attaagcaaa atttcggcat taaccgtgac ggcgttcgaa ctcaaccatg aagtctgtca
     1441 gcgctttaac gccttccagc ggcatggcgt tataaataga agcgcgcatt ccgccgacca
     1501 cacggtgacc tttcagtgca tgaaggccag cagcaaaaga ctcttcaagg aacaatttgt
     1561 caagcgcact gtccgccaac tggaacggca cgttcatccg cgaacggtta gctttcgcca
     1621 cgtcattgcg gtagaaatcg ctgttatcaa tcaccccata tagcagttct gctttttgct
     1681 gattgatttt atccatttca gctacaccgc cgttcgcttt cagccattta aagaccagac
     1741 cagatagata ccaggcaaat gtcggcggcg tgttaaacat ggagccgtta tcgttgaaga
     1801 tggaataatc cagtatcgac ggacacgcga tattcgcttt gcccagcaaa tcttcacgaa
     1861 cgatgacgat tgtcaggcca gccgggccga tatttttctg cgcgccagcg taaatcacgc
     1921 cataacggct gacgtcaatc ggacgggaaa gaatggttga agagaagtcg gcggcgacca
     1981 ccacatcttt gccgaagtct ggcgtttcgt cgatggcgat accgtcgatg gtttcattcg
     2041 ggcagtaatg catataagca gcattatcag agagttgcca ttcacgcatt ggcttaaccg
     2101 cgcgcagacc atcaacagtc actttggcgt caaagacatt aggcgtgcag tattttttcg
     2161 cttctttaat ggcacttgcc gcccagtaac cggcatcaac ataatctgcg gtggttttat
     2221 caccgagaat attcagcggt accgcagcaa actgaccgcg accaccgcca tggcagaata
     2281 ataccttgta gttggagggg acattaagaa gatcgcgaaa atccttctcg gcttcctctg
     2341 caacctgaat gaactctttg ccacggtgac tcacttccat caccgacgta ccaagaccgt
     2401 tccagtcgcg cagttcctgt tgagcctgtt taagcacctc tgccggtagc attgccggac
     2461 cagaactaaa attgaagatt tgagccattt cccctcacca cgttgcgttg ctattaaccc
     2521 gcggtcaatc gattgcgacc acaggtaata cattattcct gtggctatcg gttttatcat
     2581 tcagtgacac acatcgcaat gagtaaaacc gttgagggca aaaatgaggc ttgcatcaca
     2641 caaaagaatc tctcggatta ttagcaaaaa gccgatgttt ttactgtcag gtcacccgtt
     2701 ttgcccgact tgccttcgtc cattctggat ttgcacagcg cggacataat tgtttttccc
     2761 ccgctttcat atgaatgatt tcactacaac gaatacaggc atagtccgcc gactccggca
     2821 ccactttgta acgctgcgta caatcactgg gtaaaataag taaacccggt ttcaccttgt
     2881 catcttcaaa gaaatagaca ctgatttgcc cgttggtttc gagaatcgcc agacgtacct
     2941 gccccagctg ctccacgcca cgcaatcgta gctccataaa gaactcaaat tccgtcatgt
     3001 tggagttatt gagtttcgac caggccagct cgccatcttc aataataaca accggcttac
     3061 cttccagtag atcttccagt ttttcactgt gcgccatcaa ccacattacc aggcggtata
     3121 acaacgccag agtgataaag acgataagta ccggcaccat cggcacatca tcataaaacg
     3181 ccacatctcc cgccgctgaa cccagcgtga gaatgattaa cacttcaaac agcgacatct
     3241 gtcgcacacc gcgtcttccg gtcattttga ggaacaaaaa aaccagtaca aaggtataaa
     3301 gactacgtag tgcaacttcg ccaaggaaat caaaaggcac tttatcaaat gccatacggt
     3361 ggagatcgaa cgccttcatt tttatgctct tatcgtggtt tattcgctaa aagcatagtc
     3421 aacagttcat cggcatggcg gcggtgatag cgcctttgag ataaaacccg tattattgcg
     3481 cgctttccgt acgactaaag tgattttcat gacgcaaaca tttatccccg gcaaagatgc
     3541 cgctctggaa gattccatcg ctcgcttcca gcaaaaactt tcagacctcg gctttcagat
     3601 tgaagaggcc tcctggctga atcctgtgcc taacgtctgg tctgtacata ttcgcgacaa
     3661 agagtgcgca ctgtgtttta ccaacggtaa aggcgcaacc aaaaaagcgg cgctggcttc
     3721 tgcacttggt gaatatttcg agcgtctctc aaccaactac ttttttgctg atttctggct
     3781 gggcgaaacc atcgccaacg gtccgttcgt gcattatccc aacgaaaaat ggttcccact
     3841 gaccgaaaat gacgatgtgc cagaaggact actcgatgac cgtctgcgcg cgttttatga
     3901 tccggagaat gaactgaccg gcagcatgct gattgaccta caatccggta acgaagatcg
     3961 tggtatttgc ggcctgccgt ttacgcgtca gtccgacaat cagaccgttt atattccgat
     4021 gaatatcatt ggtaacctgt acgtctccaa cggtatgtcc gcaggtaata cccgcaacga
     4081 agcacgcgtt cagggattgt ctgaagtttt cgaacgctac gtgaaaaacc gcattattgc
     4141 tgaaagcatc agcctgccag agatcccggc agacgtgctg gcgcgttacc cagcggtggt
     4201 tgaagcgatc gaaacactgg aagcagaagg tttcccgatc ttcgcatatg atggttccct
     4261 tggcggccag tatccggtga tttgcgtggt actgttcaat cctgctaacg gtacctgctt
     4321 tgcctctttc ggtgcgcatc ctgattttgg cgtagcactg gaacgtaccg tgaccgagct
     4381 gctgcaaggt cgtggcctga aagatttgga tgtgtttact ccgccaacct tcgatgatga
     4441 agaagtcgct gaacatacca acctcgaaac gcactttatc gattccagcg gtttaatctc
     4501 ctgggacctg ttcaagcagg atgccgatta tccgtttgtg gactggaatt tctccggcac
     4561 cacggaagaa gagtttgcta cgctgatggc tatcttcaac aaagaagata aagaagttta
     4621 tattgccgat tacgagcatc tgggcgttta tgcttgccgt attatcgtgc ctggcatgtc
     4681 cgatatttat ccggctgaag atctgtggct cgcgaataac agtatgggca gccatttacg
     4741 tgaaacgatt ctttcgctac caggcagcga gtgggaaaaa gaagattacc tgaacctcat
     4801 cgagcaactg gatgaagaag gttttgatga ctttacccgc gtgcgtgagc tgttgggtct
     4861 ggcgaccggg tcggataacg gttggtacac cctgcgtatc ggtgaattaa aagccatgct
     4921 ggcgctggct ggtggcgatc tggaacaggc tctggtctgg accgaatgga cgatggagtt
     4981 taactcatca gtatttagtc cggaacgcgc caactattat cgctgcctgc aaacgttgtt
     5041 attactggca caggaagaag atcgccagcc gctgcaatat ctgaatgcgt ttgttcgcat
     5101 gtacggcgca gatgccgtgg aagccgccag tgcggcaatg agcggcgaag cggcgtttta
     5161 cggcctgcaa ccggtagata gcgatctgca cgcgtttgct gcacatcagt cgttgttgaa
     5221 ggcctacgaa aagctgcagc gcgccaaagc agcattctgg gcaaaataaa atcaaatagc
     5281 ctacgcaatg taggcttaat gattagtctg agttatatta cggggcgttt ttttaatgcc
     5341 ccgctttaca tatatttgca ttaataaaat aattgtaatt ataaggttaa atatcggtaa
     5401 tttgtattta ataaatacga tcgatattgt tactttattc gcctaatgct cccttttaat
     5461 taactatttt agcggaggat gcggaaaaaa ttcaactcat ttgttaattt ttaaaattta
     5521 tttttatttg gataatcaaa tatttactcc gtatttgcat aaaaaccatg cgagttacgg
     5581 acctataagc caggcgagat atgatctata tcaatttctc atctataatg ctttgttagt
     5641 atctcgtcgc cgacttaata aagagagagt tagtgtgaaa gctgacaacc cttttgatct
     5701 tttacttcct gctgcaatgg ccaaagtggc cgaagaggcg ggtgtctata aagcaacgaa
     5761 acatccgctt aagactttct atctggcgat taccgccggt gttttcatct caatcgcatt
     5821 cgtcttctat atcacagcaa ccactggcac aggcacaatg cccttcggca tggcaaaact
     5881 ggttggcggc atttgcttct ctctggggct gattctttgt gttgtctgcg gagctgatct
     5941 ctttacttcc accgtgttga ttgttgttgc taaggcgagt gggcgcatca cctggggtca
     6001 gttggcgaaa aactggctaa atgtctattt tggcaacctg gtcggcgcac tgctgtttgt
     6061 acttttaatg tggctttccg gcgagtatat gaccgcaaat ggtcaatggg gactaaacgt
     6121 cctacaaacc gccgaccaca aagtgcacca tacttttatt gaggccgtct gtcttggtat
     6181 cctggcaaac ctgatggtat gtctggcagt atggatgagt tattctggcc gcagcctgat
     6241 ggacaaagcg ttcattatgg tgctgccggt cgcgatgttt gttgccagcg gttttgagca
     6301 cagtatcgca aacatgttta tgatcccgat gggtattgta atccgcgact tcgcatctcc
     6361 ggaattctgg accgctgtcg gttctgcacc ggaaaatttt tctcacctga ccgtgatgaa
     6421 cttcatcact gataacctga tcccggttac gatcggtaat attatcggcg gtggtttgtt
     6481 ggttgggttg acatactggg tcatttacct gcgtgaaaac gatcaccatt aatggttgtc
     6541 gaagtacgca gtaaataaaa aatccactta agaaggtagg tgttacatgt ccgagcttaa
     6601 tgaaaagtta gccacagcct gggaaggttt taccaaaggt gactggcaga atgaagtaaa
     6661 cgtccgtgac ttcattcaga aaaactacac tccgtacgag ggtgacgagt ccttcctagc
     6721 tggcgctact gaagcgacca ccaccctgtg ggacaaagta atggaaggcg ttaaactgga
     6781 aaaccgcact cacgcgccag ttgactttga caccgctgtt gcttccacca tcacctctca
     6841 cgacgctggc tacatcaaca agcagcttga gaaaatcgtt ggtctgcaga ctgaagctcc
     6901 gctgaaacgt gctcttatcc cgttcggtgg tatcaaaatg atcgaaggtt cctgcaaagc
     6961 gtacaaccgc gaactggatc cgatgatcaa aaaaatcttc actgaatacc gtaaaactca
     7021 caaccagggc gtgttcgacg tttacactcc ggacatcctg cgttgccgta aatctggtgt
     7081 tctgaccggt ctgccagatg catatggccg tggccgtatc atcggtgact accgtcgcgt
     7141 tgcgctgtac ggtatcgact acctgatgaa agacaaactg gcacagttca cttctctgca
     7201 ggctgatctg gaaaacggcg taaacctgga acagactatc cgtctgcgcg aagaaatcgc
     7261 tgaacagcac cgcgctctgg gtcagatgaa agaaatggct gcgaaatacg gctacgacat
     7321 ctctggtccg gctaccaacg ctcaggaagc tatccagtgg acttacttcg gctacctggc
     7381 tgctgttaag tctcagaacg gtgctgcaat gtccttcggt cgtacctcca ccttcctgga
     7441 tgtgtacatc gaacgtgacc tgaaagctgg caagatcacc gaacaagaag cgcaggaaat
     7501 ggttgaccac ctggtcatga aactgcgtat ggttcgcttc ctgcgtactc cggaatacga
     7561 tgaactgttc tctggcgacc cgatctgggc aaccgaatct atcggtggta tgggcctcga
     7621 cggtcgtacc ctggttacca aaaacagctt ccgtttcctg aacaccctgt acaccatggg
     7681 tccgtctccg gaaccgaaca tgaccattct gtggtctgaa aaactgccgc tgaacttcaa
     7741 gaaattcgcc gctaaagtgt ccatcgacac ctcttctctg cagtatgaga acgatgacct
     7801 gatgcgtccg gacttcaaca acgatgacta tgctatcgct tgctgcgtaa gcccgatgat
     7861 cgttggtaaa caaatgcagt tcttcggtgc gcgtgcaaac ctggcgaaaa ccatgctgta
     7921 cgcaatcaac ggcggcgttg acgaaaaact gaaaatgcag gttggtccga agtctgaacc
     7981 gatcaaaggc gatctcctga actacgatga agtgatggag cgcatggatc acttcatgga
     8041 ctggctggct aaacagtaca tcactgcact gaacatcatc cactacatgc acgacaagta
     8101 cagctacgaa gcctctctga tggcgctgca cgaccgtgac gttatccgca ccatggcgtg
     8161 tggtatcgct ggtctgtccg ttgctgctga ctccctgtct gcaatcaaat atgcgaaagt
     8221 taaaccgatt cgtgacgaag acggtctggc tatcgacttc gaaatcgaag gcgaataccc
     8281 gcagtttggt aacaatgatc cgcgtgtaga tgacctggct gttgacctgg tagaacgttt
     8341 catgaagaaa attcagaaac tgcacaccta ccgtgacgct atcccgactc agtctgttct
     8401 gaccatcact tctaacgttg tgtatggtaa gaaaactggt aacaccccag acggtcgtcg
     8461 tgctggcgcg ccgttcggac cgggtgctaa cccgatgcac ggtcgtgacc agaaaggtgc
     8521 tgtagcgtct ctgacttccg ttgctaaact gccgtttgct tacgctaaag atggtatctc
     8581 ctacaccttc tctatcgttc cgaacgcact gggtaaagac gacgaagttc gtaagaccaa
     8641 cctggctggt ctgatggatg gttacttcca ccacgaagca tccatcgaag gtggtcagca
     8701 cctgaacgtt aacgtgatga accgtgaaat gctgctcgac gcgatggaaa acccggaaaa
     8761 atatccgcag ctgaccatcc gtgtatctgg ctacgcagta cgtttcaact cgctgactaa
     8821 agaacagcag caggacgtta ttactcgtac cttcactcaa tctatgtaat tagatttgac
     8881 tgaaatcgta cagtaaaaag cgtacaataa aggctccacg aaagtggggc cttttttagc
     8941 acgagagcct tttttgtcag ctatctatac tttaaggtga ctgccaaaac agactcgacg
     9001 tagccttcga gctgcgcacc aacacggcct cagatgggcc acatctggag aaacaccgca
     9061 atgtcagtta ttggtcgcat tcactccttt gaatcctgtg gaaccgtaga cggcccgggt
     9121 attcgcttta tcaccttttt ccagggctgc ctgatgcgct gcctgtattg tcataaccgc
     9181 gacacctggg acacgcatgg cggtaaagaa gttaccgttg aagatttgat gaaggaagtg
     9241 gtgacctatc gccactttat gaacgcttcc ggcggcggcg ttaccgcatc cggcggtgaa
     9301 gcaatcctgc aagctgagtt tgttcgtgac tggttccgcg cctgcaaaaa agaaggcatt
     9361 catacctgtc tggacaccaa cggttttgtt cgtcgttacg atccggtgat tgatgaactg
     9421 ctggaagtaa ccgacctggt aatgctcgat ctcaaacaga tgaacgacga gatccaccaa
     9481 aatctggttg gagtttccaa ccaccgcacg ctggagttcg ccaaatatct ggcgaacaaa
     9541 aatgtgaagg tgtggatccg ctacgttgtt gtcccaggct ggtctgacga tgacgattca
     9601 gcgcatcgcc tcggtgaatt tacccgtgat atgggcaacg ttgagaaaat cgaactcctc
     9661 ccctaccacg agctgggcaa acacaaatgg gtggcaatgg gtgaagagta caaactcgac
     9721 ggtgttaaac cacctaagaa agagaccatg gaacgcgtga aaggcattct tgagcagtac
     9781 ggtcataagg taatgttcta atttccccgt aaagcggcaa ctttattgag ttgccgcttt
     9841 tttattccgc gcttcattct gctacagtgt ttttaacaca ttttata
//
LOCUS       DAJVUJ010000159         9653 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.159, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000159 DAJVUJ010000000
VERSION     DAJVUJ010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9653
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.159"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(1..1093)
                     /gene="fdeC"
                     /locus_tag="OQQ48_003484"
                     /pseudo
     CDS             complement(1..1093)
                     /gene="fdeC"
                     /locus_tag="OQQ48_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308363.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="intimin-like adhesin FdeC"
     gene            2209..2310
                     /locus_tag="OQQ48_003485"
     CDS             2209..2310
                     /locus_tag="OQQ48_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1954128.1"
                     /translation="MKENKVQQISHKLINIVVFVAIVEYAYLFLHFY"
     gene            2674..2937
                     /locus_tag="OQQ48_003486"
     CDS             2674..2937
                     /locus_tag="OQQ48_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HCS1954129.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKKGS"
     gene            2937..3077
                     /gene="ykgO"
                     /locus_tag="OQQ48_003487"
     CDS             2937..3077
                     /gene="ykgO"
                     /locus_tag="OQQ48_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HCS1954130.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(3112..3339)
                     /locus_tag="OQQ48_003488"
                     /pseudo
     CDS             complement(3112..3339)
                     /locus_tag="OQQ48_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414829.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4163..4705
                     /gene="ecpR"
                     /locus_tag="OQQ48_003489"
                     /gene_synonym="matA"
     CDS             4163..4705
                     /gene="ecpR"
                     /locus_tag="OQQ48_003489"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HCS1954131.1"
                     /translation="MECQNRSDKYIWSPHDAYFYKGLSELIVDIDRLIYLSLEKIRKD
                     FVFINLNTDSLSEFINRDNEWLSAVKGKQVVLIAARKSEALANYWYYNSNIRGVVYAG
                     LSRDIRKELAYVINGRFLRKDIKKDKITDREMEIIRMTAQGMQPKSIARIENCSVKTV
                     YTHRRNAEAKLYSKIYKLVQ"
     gene            4731..5366
                     /gene="ecpA"
                     /locus_tag="OQQ48_003490"
                     /gene_synonym="matB"
     CDS             4731..5366
                     /gene="ecpA"
                     /locus_tag="OQQ48_003490"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HCS1954132.1"
                     /translation="MTHWDITSSIQLGKKCNEKKVLAIALVTVFTGMGVAQAADVTAQ
                     AVATWSATAKKDTTSNLVVTSLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKL
                     TSRLITNTLTQLDTSGSTLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYN
                     ASNRTTAQDGFTFTIISGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            5424..6092
                     /gene="ecpB"
                     /locus_tag="OQQ48_003491"
                     /gene_synonym="yagY"
     CDS             5424..6092
                     /gene="ecpB"
                     /locus_tag="OQQ48_003491"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HCS1954133.1"
                     /translation="MKKHLLPLALLFSGISPAQALDVGDISSFMNSDSSTLSKTIKNS
                     TDSGRLINIRLERLSSPLDDGQVISMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGSLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            6118..8640
                     /gene="ecpC"
                     /locus_tag="OQQ48_003492"
                     /gene_synonym="yagX"
                     /pseudo
     CDS             6118..8640
                     /gene="ecpC"
                     /locus_tag="OQQ48_003492"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414825.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
     gene            8630..>9653
                     /gene="ecpD"
                     /locus_tag="OQQ48_003493"
     CDS             8630..>9653
                     /gene="ecpD"
                     /locus_tag="OQQ48_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HCS1954134.1"
                     /translation="MRVNLLITMIIFALIWPVTALRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYTGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDASGFYGATVTSGGAKWMHGMLS
                     DAFYQYMQQMPVGSSFTMTINACQTSVNYDASSGARCKDQASGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGSLSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNL"
BASE COUNT         2628 a         2369 c         2255 g         2401 t
ORIGIN      
        1 caatacggat cacttcagat ttgcggtact caagaacgat gttgttatta cgctcaacca
       61 ggtcatagcg gctgcctgcc agcattcgac gctcgcgaat gctgtctgta tcgagttgtt
      121 tctccagagg ttcgccaatc cgataattaa cttccaggcc aaagcgagtg tcattctcac
      181 cactcttgcc ctgcttatgc ccggcactca gggtcagaag aggcacaggc gtgtaattca
      241 cttcagcgga aatcgcatgt ggatctttct ggcgtttatc tttgccaaac agcccaactt
      301 catcgccata atactgctca tacatcaggc ttgcgccaag ctgcggtcag gcgggtaaat
      361 agccctcagc acgaatatcc cagccattcg ccgggcgttc ctgataatcc tcaatatccg
      421 gcgatttttt ccagccagaa gcccgaatat aaccattggc gctcagtttc agataatcgc
      481 gccagtattc cgcaccaaca ccaatgcggg tatgactacg ggataaatca tggtcgataa
      541 aggtgttcac cccccccatt cagtcatttc ctgaaaaatg acgccagcca aaaccaatat
      601 ttgactgagt acgatcgtct gtacgatgta ttgccccctg agtgaacaac atatttgtcg
      661 gcgtatcata aatcggataa agcatttcca gcgaagaatc cttcagcgag aaatctttat
      721 cgacattcag tttgacgcgc gctgtaccat atttcccgag ccactcctgt atttcctggt
      781 tagctttagc ggtggccatt ccggtaataa aattacgtgt cgcatcgcta tctggctgac
      841 tgcttaaaaa tgtcccggca tttgcggcaa acgacgcgac atttttctcc acgttattat
      901 cagcagttac cgtagtattt cccatgctca accgtggctg aaccgcatgc tgcgcacgtg
      961 ccgccattac tggggtaaag gtgacagcga gtggaaaaag aacctgaaca gagatatttg
     1021 cccacgccac gcagcgggcc agaactgaat aacgaaatcg tggttgttta tgacctgttt
     1081 tataacgtga cattgagata atttcctgat gaacgaatta acgaacatag gcagacacaa
     1141 ataatggcgt ctgcttgtca gaaaaaccgg ggatttcccg gtttcctttt ccgcagaaaa
     1201 ggaaatacct ttatgtaaga aagtgcaccg ccacctgata tatccagtgt ctgtagccac
     1261 tcatcaggtg gttgggcgcg ggattatatt tttatttatg ggtaaaaatt tagctttttg
     1321 tgcaagtcat taaatagata cctaagttat atttcttact ctgcggacag tgaataattc
     1381 attattcaga ctgaataata atgacctgcc tttaattagc aactattgga aatacgtcga
     1441 agtaggtata tacgccagga taaaccgata tatttattat ggatattcat tttccttcat
     1501 cggactgtaa attgcgggga tatcgaactt acactactca tcgcaacact caaacagatt
     1561 tatgaatttc gttcataacc cctgacaaat cctgccgtct aatcgagctt ttccctttac
     1621 gttatgcttc tcatgaacga gaacacaact tattcatgaa ttaaccattc cggataaact
     1681 atggaatcaa attcaggcat cgaaaggaac tggctggttt ccgttaatag attaaatatc
     1741 tattactctc ccgagagtaa aactatcaaa tcaatgagta accgatacag cctcacaaat
     1801 tctttcagat aattaacccg tcactgcggc acgttctctc gtaaggcgtg cctgcagtgt
     1861 aatccagtat gtatccggca tagttgacgg tttaatagaa ctcaattcct ctccctggtc
     1921 accaacaacg atatcttgta taacacccca ggagatacga cattatgatg agatgcgata
     1981 tgctcctcag caccaatcaa cgccaggact gggccagtct tgagatcaga ctcattcccg
     2041 ggactgactt cagtcgacat atatcaatga cgttggtgca ataaaagata gccgatgtaa
     2101 tacatctatt caaatttgtg atacatgtca aaaaactggt tgaccaaact cgttatttat
     2161 ataagggcac ttacgaagtg cgctcttttt taaagcgagg aagtaccaat gaaagagaat
     2221 aaagtacaac aaatcagtca taaactaatt aatatcgttg tttttgtcgc aattgtagaa
     2281 tacgcctatt tatttctcca tttctattaa taacggcaat aaactgttca cttcagtgat
     2341 atttaaaata tgcattctct ccctttttgt aagtaattat tatatccgtg ggagaggaat
     2401 acacgctgtc aggtaatcaa tcatactgcg ataaatcatc ggccagtaaa gtggagataa
     2461 cctccattgt cgaaaaatcc atactctcag cgaaaccatc atcaatcact catccaggcg
     2521 tttatgggag cgtcgccaac ggctgttaac aatgccagac ttccccgttg cggaaattcc
     2581 acataccact aatggtcaca gggattgggt gttgaaatga tccggatgag catgtatctt
     2641 tatggttatg ctataacata acaggtaaaa atgatgaagc ccaatatcca tcctgagtat
     2701 cgtactgtgg tgttccacga caccagtgtt gatgagtact ttaaaatcgg ctcgactatc
     2761 aaaacagacc gtgagattga gctggatggc gtaacgtatc cgtacgtgac aattgatgtc
     2821 tcttctaaat cgcacccgtt ctatacaggg aagctgagaa cagtggcatc agaaggaaat
     2881 gttgcacgat tcacccaacg ttttggtcgt tttgttagca cgaaaaaggg gtcgtgatga
     2941 aagttcttaa ctctctgcgt accgcaaaag aacgccatcc agactgtcag attgtgaagc
     3001 gaaaaggacg gctatatgtg atttgtaaat ctaatccacg ttttaaggcc gttcaggggc
     3061 gtaagaaaaa acgttgattc aaaattcgac ggattaacga tatttgtctg attaataatc
     3121 agatcggatt aatgttggtg tgtttataac accaacatta attttcctgg ggatatattc
     3181 ttcctgttca tttgaggcca actgcctgac gtttctctac gaatattcca ttatcttaat
     3241 gttgacttgt tgaccagctt cgcccctgta tgctggcatc aactctcttt tagactgaac
     3301 acgccactca gtctcctccc tttgcggcgc agcctgcatt ttcactcaaa ctgttaagat
     3361 gataaatgtg gtaaatctgt tggtactaac ataaaaacgt ttacgccaca ggaacagtct
     3421 gatccaccgg taaccccgtc gccgacgttc gagtgccagt tagagtaacg cgcacagata
     3481 actgcactca gtgccctgac aaagaggcca tcgttcctgt gacagctggc agccttcgtt
     3541 taacttcact taatctggct cttgagggct taccgaacag atgacgtaca tacgcccgtt
     3601 caattttcca tcacttattg taatgaacac ctataaccat tttgtgcggc atgttaatcc
     3661 attaaaattt cttactgatt ggcaaatcat cttcaatgac agctcattat agttttatat
     3721 tctatccctt acccttaaaa cttgtttttt tactagtcca tcacacagcg cattaagact
     3781 attcctaaca cttcagggca aagttcctga ccaatataaa atgcaagtaa gaattgaacg
     3841 ttatattgcc aataacctta tgaaaccaaa tgtctttttc ttcttatcaa aaaagcaata
     3901 ttttcagttt ttctaaatat tgacttaacc attgaattcc ttttccgttc acatattgac
     3961 actcatcgaa aaaaaaaaca taaatttaag cccaatcgaa aataattaaa cttaatctcg
     4021 tttaaccttt attgatatgt actacgtatc ttatttactt ccggtttact aaggaaactg
     4081 aatgcacctg taaaaattac aggtttggaa agtagtgaca tggcaaagtg attacagtag
     4141 ggactatgag gttaaaaacc atatggaatg tcaaaaccgt tctgataaat acatctggtc
     4201 tccccatgac gcctacttct ataaaggact atctgaactg attgtggata tcgacagatt
     4261 aatttatctg tcgttggaga aaattagaaa agatttcgtg tttatcaatc tcaatacgga
     4321 ttctttatct gaatttataa accgtgataa tgaatggtta tccgcggtaa aggggaaaca
     4381 ggtcgtattg attgcggcca gaaagtcaga agccttagca aattattggt actacaacag
     4441 caatattagg ggcgtggtat acgctggact gagtcgtgat attagaaaag aactggccta
     4501 tgtgattaat ggcaggttcc tgagaaaaga tattaagaaa gataaaatca cggaccggga
     4561 aatggaaatt atccgcatga cggcccaggg aatgcaacct aaatcgattg ccagaattga
     4621 aaattgtagt gtgaagacag tgtataccca tcggcgtaat gctgaggcca agctgtactc
     4681 aaaaatatat aagttggttc agtaaactcc aggcaagtta gttttaaaaa atgactcact
     4741 gggacatcac gtcctcaatt caactcggga agaaatgcaa tgaaaaaaag gttctggcaa
     4801 tagctctggt aacggtgttt accggcatgg gtgtggcgca ggctgctgac gtaacagctc
     4861 aggctgtagc gacctggtcg gcaacagcca aaaaagacac caccagtaat ctggttgtga
     4921 cgtcactcgg tagcttggcg ttccagtatg ccgaaggcat taaaggtttt aactcacaga
     4981 aaggtctatt tgacgtggct atcgagggtg actcaacggc taccgccttt aaactgacct
     5041 cacgtcttat caccaacaca ttaactcagt tggatacctc aggttccaca ctgaatgtgg
     5101 gcgtggatta taacggcgcg gcagtcgaaa aaactggcga taccgtgatg atcgataccg
     5161 ccaacggcgt actgggcggc aaccttagcc cgctggctaa cggttacaat gccagcaatc
     5221 gtaccaccgc acaggatggt ttcaccttca ccatcatcag cggcaccacc aatggtacca
     5281 ccgcagtaac cgattacagc actctaccgg aaggcatctg gagcggcgac gttagcgtac
     5341 agttcgacgc gacctggacc agttaatctc tctgatgtac cagcaggggt agcccccctg
     5401 ctttattcct ggacggaatt gttatgaaaa agcaccttct gcctctcgct ctgctgtttt
     5461 ccggaatatc tccggcccag gcgctggatg tcggcgatat atcatcgttt atgaacagtg
     5521 acagcagcac gctgagcaaa acgatcaaaa acagtaccga cagtggtcgc cttatcaata
     5581 tccgtctcga acggctctct tcaccgcttg acgacgggca ggttatctca atggacaagc
     5641 cggatgagtt gctactcact cccgccagct tgctgctacc cgcccaagcc agcgaagtga
     5701 tccgcttctt ctataaggga cccgcagatg aaaaagagcg ctactaccgc attgtctggt
     5761 ttgatcaggc cctcagtgat gcgcagcgcg ataatgccaa ccgcagcgct gtggccactg
     5821 cttccgcccg catcggcacc attctggtcg tcgcccctcg tcaggcgaac taccactttc
     5881 agtacgccaa cggctccctg acaaatacag gaaatgcgac gctgcggatc ctcgcctacg
     5941 gcccctgcct gaaagccgcc aacggtaagg agtgtaaaga gaattactac ctgatgccgg
     6001 gcaagtcgcg tcgttttacc cgcgtggaca ctgcggataa caaaggacgg gttgcacttt
     6061 ggcagggtga taagttcatt cccgtgaaat agatagctgt gcagatggat aacgacaatg
     6121 cctttacgac ggttctcccc aggactgaaa gcccagtttg ccttcggcat ggtctttttg
     6181 ttcgttcagc ccgatgccag cgctgctgac ataagtgcgc agcaaatagg tggggtgatt
     6241 attccgcagg ccttcagtca ggcgcttcag gacggcatga gcgtcccgct ctatattcat
     6301 ctcgccggta gtcagggtcg ccaggacgat cagcgaatcg gcagcgcttt tatctggctg
     6361 gatgatggac agctacgcat ccggaaaata cagctggaag agagtgaaga taacgccagt
     6421 gtcagcgaac aaactcgaca gcagctgatg gctctggcga acgccccgtt caatgaggcc
     6481 cttaccatcc cctgactgac aacgcgcagt tggatctcag cttgcgccaa ctgctgctgc
     6541 agctggtggt caagcgcgaa gcgctgggca ccgtactacg ctcacgtagc gaagacatcg
     6601 ggcagtccag tgttaacacc ctcagcagta atctgagcta taacttgggc gtctataaca
     6661 accagttgcg taacggcggg agcaacacat ccagctatct gtcgctgaat aacgttactg
     6721 cgctgcgcga acatcatgtg gtgctcgacg gctcgctgta cgggatcggt agcggtcaac
     6781 aggacagtga attatataaa gcgatgtatg aacgcgattt tgccggtcac cgatttgccg
     6841 gtggaatgct cgacacctgg aacttgcagt cattagggcc gatgaccgcc atttcagcag
     6901 ggaagattta cggcctttcc tggggaaacc aggccagctc caccatcttc gacagcagcc
     6961 agtcagccac gccagtgatc gcctttttac cggcggcggg cgaagtacat ctcacccgtg
     7021 atgggcggct actaagcgtt cagaacttca ctatgggcaa tcatgaagtg gatacccggg
     7081 gtctaccgta cggtatttac gatgtggaag ttgaggtgat cgttaacggt cgcgtgatca
     7141 gcaaacgcac tcaacgggtc aataagctgt ttagccgggg cgcggcgtcg gtgcaccact
     7201 ggcgtggcag gtatggggcg gtagctttca tatggatcgc tggtcggaaa acgggaaaaa
     7261 gacgcgacca gctaaagaga gttggctagc aggtgcctcg acctccggct cattgagtac
     7321 gcttagctgg gcggcaacgg gatatggata cgataatcag gcggtgggtg aaacccgtct
     7381 gacgctgccg cttggggggg cgatcaacgt taacctgcaa aatatgctgg ccagtgacag
     7441 ctcatggagc agcatcggca gcatcagcgc tactctaccg ggaggcttta gttcgctgtg
     7501 ggttaaccag gaaaaaaccc gtattggcaa tcaattgcga cgtagcgatg ccgacaaccg
     7561 tgcaatcggc ggcacactca acctgaactc actgtggtcg aagctgggca cattcagcat
     7621 cagctacaat gatgaccgcc gttacaacag ccattattac acggcagatt actatcaaaa
     7681 tgtctacagc ggtacctttg gttcgcttgg cctgcgggcc ggtattcagc gctataacaa
     7741 cggcgacagc aacgccaata cagggaaata tatcgctctc gatctctcgc taccactggg
     7801 caactggttt agcgcaggga tgacccatca aaacggctac accatggcaa acctgtcagc
     7861 acgcaaacag tttgatgaag ggaccattcg cactgttggt gccaatctgt cacgtgccat
     7921 ctccggcgat accggtgatg acaaaaccct cagcggtggg gcgtatgcac agttcgacgc
     7981 tcgttacgcc agcggaacgc tgaacgtcaa tagcgcggcg gacggctacg tcaataccaa
     8041 cttgaccgcc aatggcagcg tcggctggca gggtaaaaac attgctgcca gcggcggact
     8101 gatggcaacg ctggggtgat attcaacacc gggctggagg acgacggtca gatcagcgcc
     8161 aaaatcaacg ggcggatttt cccgcttaac ggcaagcgta actatctccc gctctctccc
     8221 tatggaagat atgaggtgga gttacagaac agcaaaaact cactcgacag ttacgatatc
     8281 gtcagcggcc gcaaaagtcg tctgactctc tatccaggca atgtcgctgt cattgagcca
     8341 gaggtgaagc agatggttac cgtctccggt cgtatccgtg cggaagacgg cacactgctg
     8401 gctaacgcac ggattaacaa ccatatcggc cgaacccgaa ccgatgaaaa cggcgagttt
     8461 gtcatggacg tggataagaa ataccccact atcgattttc gctacagtgg caataaaacc
     8521 tgcgaagtgg ctctggaact caaccaggcg cgcggtgccg tctgggtcgg tgatgtggtc
     8581 tgcagcggcc tctcatcgtg ggcggcggtg acgcagacag gagaagagaa tgagagttaa
     8641 cctactaata acgatgataa ttttcgcgct aatctggcca gtaactgcgc tcagagcggc
     8701 agtgagcaaa acaacctggg cggatgcacc ggcacgcgag tttgtgtttg tcgaaaacaa
     8761 ctcagacgac aactttttcg tcactcctgg cggggcgctg gatccgcgcc tgaccggtgc
     8821 caaccgctgg accggtttaa aatacactgg ttcaggaacc atctatcagc aaagcctcgg
     8881 ctacattgat aacggttaca acaccggcct ttataccaac tggaagtttg atatgtggct
     8941 ggaaaattcg ccggtttcat ctcctttaac tggcttacgc tgcatcaact ggtacgcagg
     9001 gtgtaatatg accaccagtc ttatcctgcc gcaaaccacc gacgccagtg gattttatgg
     9061 cgcgacggtg accagcggcg gcgcgaagtg gatgcacggc atgttgtcag acgcgtttta
     9121 ccagtatatg caacaaatgc ccgtcggcag cagctttaca atgaccatca atgcctgtca
     9181 gacctctgtg aactatgacg ccagcagcgg cgcgcgttgt aaggatcagg cctccggcaa
     9241 ctggtatgtt cgcaatgtca cccatacgaa agcagcaaat cttcggttaa taaataccca
     9301 ctcgctggcg gaggtattta tcaacagcga cggggtgccg accctgggcg aagggaacgc
     9361 cgactgccgg acacagacca tcggcagcct ttcaggatta agttgtaaga tggtcaacta
     9421 taccctgcaa acaaacggac tcagcaacac ctcaatccat atattcccgg cgatcgccaa
     9481 ctcgtcgtta gcctcggccg tcggggcgta cgatatgcag ttcagtctga atggcagttc
     9541 atggaaaccg gtgagcaata ccgcctatta ctacaccttc aacgagatga agagcgcaga
     9601 ctcgatctat gttttcttct cgagcaactt ctttaagcag atggtgaacc tcg
//
LOCUS       DAJVUJ010000160         9488 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.160, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000160 DAJVUJ010000000
VERSION     DAJVUJ010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9488
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.160"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..191
                     /locus_tag="OQQ48_003494"
     CDS             <1..191
                     /locus_tag="OQQ48_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1954135.1"
                     /translation="AHFMMSGGNILALQKILGHHDIKMTMRYAHLAPDHLETALRFNP
                     LATLPSGDKVAAAVGITP"
     gene            complement(296..832)
                     /gene="rcdA"
                     /locus_tag="OQQ48_003495"
     CDS             complement(296..832)
                     /gene="rcdA"
                     /locus_tag="OQQ48_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HCS1954136.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            916..2124
                     /locus_tag="OQQ48_003496"
     CDS             916..2124
                     /locus_tag="OQQ48_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1954137.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            2124..2939
                     /gene="ybjI"
                     /locus_tag="OQQ48_003497"
     CDS             2124..2939
                     /gene="ybjI"
                     /locus_tag="OQQ48_003497"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HCS1954138.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGNGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            3025..3309
                     /locus_tag="OQQ48_003498"
     CDS             3025..3309
                     /locus_tag="OQQ48_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1954139.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYARHLASVGEGMVIEHQIDDHHH"
     gene            complement(3350..4580)
                     /gene="mdfA"
                     /locus_tag="OQQ48_003499"
                     /pseudo
     CDS             complement(3350..4580)
                     /gene="mdfA"
                     /locus_tag="OQQ48_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
     gene            4865..5461
                     /gene="ybjG"
                     /locus_tag="OQQ48_003500"
     CDS             4865..5461
                     /gene="ybjG"
                     /locus_tag="OQQ48_003500"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCS1954140.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            5519..6277
                     /gene="deoR"
                     /locus_tag="OQQ48_003501"
     CDS             5519..6277
                     /gene="deoR"
                     /locus_tag="OQQ48_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HCS1954141.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPHSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(6324..7515)
                     /gene="dacC"
                     /locus_tag="OQQ48_003502"
                     /pseudo
     CDS             complement(6324..7515)
                     /gene="dacC"
                     /locus_tag="OQQ48_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
     gene            7762..8387
                     /gene="gstB"
                     /locus_tag="OQQ48_003503"
                     /pseudo
     CDS             7762..8387
                     /gene="gstB"
                     /locus_tag="OQQ48_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
     gene            complement(8384..>9488)
                     /gene="yliI"
                     /locus_tag="OQQ48_003504"
     CDS             complement(8384..>9488)
                     /gene="yliI"
                     /locus_tag="OQQ48_003504"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HCS1954142.1"
                     /translation="SFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLPDNHG
                     MLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWLSYSE
                     VGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYLFIAL
                     GENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQGMAMN
                     PWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAGTEQP
                     VFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRILTDR
                     GQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
BASE COUNT         2295 a         2454 c         2338 g         2401 t
ORIGIN      
        1 cggcgcactt catgatgtcg ggtggaaaca ttctggcctt acaaaaaatt ctcggacacc
       61 acgatataaa aatgactatg cgttacgcac atctggcacc ggatcatctg gaaacggcgc
      121 tccgtttcaa tcctctggca acgctgccaa gtggcgacaa agtggcggca gcggttggca
      181 ttaccccgta ataaccacca ctgaccacca acctaactta ttgtttttaa tctaacttat
      241 tgttttcatt aacccgttta cataaatggg ttttttgtta tctaaaattt atctactacc
      301 ctgcaaccct ctcaaccatc ctcaaaatct cctcgcgcga tagcggctta cggtcggtga
      361 caaaatgcag cgtcatcccc tcaataaacg catcaagcgc gcgggcggtt ccgggttcaa
      421 accattgttc gagcgtttgc tgactgcgct gcatccagtt ttgcattacc gtttttaata
      481 gcggttttcg gctagccagc gcgtagagct ggtacatcag ctccatatta tccggcgttg
      541 caacctgtga gctgtagatc atatcggtga tagcctggca tgcgccttga gcatcactaa
      601 catcgctaaa aaatgcctga tattgccggg acataatctc agtaaaactg ctgaacgcct
      661 ccagcaacaa ctcatcaatt cctgaaaaat agtaggtcat cgatcccaac ggtaccccgg
      721 caagggtagc aattttgcgg tgcgtaacag catgtattcc gtaaagtttc accgcctcca
      781 gcgtggcctg gataattttt tctcgccgtt gcggatcgtt agcgcgacgc atgtcatttc
      841 ctctgtctgc aattgtgtac aaatgtacac aaccttgcta atgttgtctt ccctcttctt
      901 attttgacgt gatctatgac cgtaaattct tcacgtaatg cattgaaacg ccgaacctgg
      961 gcgctgttta tgttcttctt tttgccaggc ctgttaatgg cgtcctgggc aacccgtacg
     1021 cctgctatcc gcgatattct ctctgtctcg atcgctgaaa tgggcggtgt tctctttggt
     1081 ctgtcgatcg gttcgatgag cggtattctc tgctcggcgt ggttagtgaa acgctttggg
     1141 acacgtaatg tcatcctggt cacgatgtcc tgcgcattga tcgggatgat gatattaagt
     1201 ctggcactct ggctgacatc gcccctgctc tttgccgttg gtctcggcgt ctttggggca
     1261 agttttggtt ctgcggaagt ggcgataaac gttgaaggtg ccgccgttga gcgagaaatg
     1321 aataaaacgg ttttgccgat gatgcacggt ttttatagcc tgggcacgct ggcaggcgct
     1381 ggtgtcggga tggcactgac ggcctttggc gttccggcaa cggtgcacat tttattggcg
     1441 gcgctggtag gtatcgcgcc tatttatatc gccattcagg caatccctga cggtacgggc
     1501 aaaaatgctg ccgatggcac ccagcatggc gaaaaaggca ttccttttta tcgcgatatc
     1561 cagttgctgt tgattggtgt tgtagtgctg gcgatggcct ttgccgaagg ttctgccaac
     1621 gactggttac ccttattaat ggttgacggt cacggtttta gccctacttc cggctcgctg
     1681 atttatgccg gttttaccct ggggatgacc gttggacgct tcactggcgg ttggttcatc
     1741 gaccgttaca gtcgcgttgc cgtggttcgg gccagtgcac taatgggggc gttgggtatt
     1801 gggctgatta tttttgtcga tagcgcctgg gtcgctgggg tgtctgttgt actctgggga
     1861 ctgggtgcct cgctgggctt cccgctgacc atttctgccg ccagcgatac cggccccgat
     1921 gcaccaaccc gcgtcagtgt ggtagcaacg accggttatc tggctttcct cgtcgggccg
     1981 ccgctgctgg gctatctcgg cgaacattat ggattacgta gtgcaatgct ggttgtactg
     2041 gcgctggtta ttctcgcggc tattgtcgcg aaagccgtcg ccaaacccga taccaaaacg
     2101 cagacggcga tggagaatag ttgatgagca ttaaattaat tgcggtagac atggatggta
     2161 ctttcttaag cgatcaaaaa acctataacc gtgagcggtt tatggctcag tatcagcaaa
     2221 tgaaagcaca aggcattcgc tttgtggtcg ccagcgggaa tcaatattat cagttgatct
     2281 ctttcttccc tgaaattgct aatgaaattg cctttgtggc tgaaaacggc ggctgggtag
     2341 tgagtgaagg caaagatgtt tttaatggcg agctgtcgaa ggatgccttt actactgtcg
     2401 tggaacattt gctgacgcgc ccggaagtgg aaattattgc ctgcggaaaa aatagcgcct
     2461 atacgctcaa aaaatatgac gatgccatga aaacggtggc ggaaatgtat tatcaccgtc
     2521 tggaatacgt tgataacttt gacaacttag aagatatttt ctttaagttt ggcctgaatc
     2581 tttccgatga actgattcca caagtacaaa aagcattaca tgaggccatc ggcgatatta
     2641 tggtgccagt ccacaccggc aacggcagca tcgatctgat tatccccggc gtacataaag
     2701 ccaatggcct tcgccaactg cagaaattat ggggaataga cgacagcgaa gtggtggtct
     2761 ttggcaatgg cggtaacgat attgagatgc tgcgtcaggc aggctttagt tttgcaatgg
     2821 aaaatgccgg cagcgcggtc gtcgcagcgg caaaataccg ggcaggctcc aataaccgtg
     2881 aaggcgtact ggatgtgatc gataaagtgc ttaaacacga agcgccattt gaccaataaa
     2941 aacggcagca cgcccggcaa cacaatacct gaatgaaggc tgcataattc gcggaaatta
     3001 acccgctgca ttatttcagg cgaaatgatt atgaaaaatt gtctactgtt gggcgcgctt
     3061 ttaatgggct ttactggtgt ggcgatggcg caaagtgtca ccgtcgatgt gccgagtgga
     3121 tacaaagtgg ttgtagttcc tgattcagtc agcgttccac aagcggtcag cgtcgcgact
     3181 gtgccgcaaa ccgtctatgt ggctcctgcg ccagctccgg cttaccgccc gcatccttat
     3241 gctcgccatc ttgccagcgt aggtgaaggg atggttatcg aacaccagat tgacgaccat
     3301 caccattaat tcgagaattc ctgatcgcac aaagcagtca ggcatttttt tacccttcgt
     3361 gagaatttcc catctgttta tcttttaaaa agataaccat cagcgacagc cacaaaattc
     3421 cgttgacaag gttgaagaga ttaaactgtc cgttgccccc gttcagccag gcatgtttgc
     3481 tgatttcaat accaacggta aagatcagca tttgcagcat tcccatcgcc gcagaaaccg
     3541 tacctttact catatcgctg gcaaacaggg ttaatcgcac cagtcccgca ttcgccagac
     3601 caataccgaa agcataaata cttaacccgg cagtcatcca taaatacgcg tgcgatgaga
     3661 taaccgttgc cgcagcagcg accaacagac caatcataat cggccagccg cccataatta
     3721 tcagcgaacg tacggtgcgg cgcgaggtca gacgcgctaa cagcaagtta cccgcaatta
     3781 acgccccgaa aataggcact tgcagcaagc catattcata gctgctcaac tgctcgccgg
     3841 taatgatgat aatcggcgac tgggcgatcc acgccagcaa tggcaggcta acgaatccca
     3901 gcgccagcgc ccccgccaca aagcggccgt tcttcagcac cagcttatag tcacgaccga
     3961 gttctttcag tgacagtttc tcgcctatac gcgtggcggt ttcaggcata gctcgttgca
     4021 gaccgaaaaa ggagatcgct gccaatgcgg caaacaaaac aaacatcccc tcccagggca
     4081 gcacatggat ccacgccgcg cccaccagcg gaccaagtag cggagcaatc agcgccacgt
     4141 tcgccatcag cgcggtgatc ttgatacaaa ccgcctcttc gaaagattcc tgaattgcgg
     4201 cgtatcccac agcgccaatg aaacagaggc ttatgccctg caagaagcgc aacagagtga
     4261 attgttcaat gttttgcgcc agtaatattg ccagacaggt gatgataaac cacaccaccc
     4321 ccgccagcat caccggacgg cgaccaatac gatccgacag cggccccagg agccattgta
     4381 aaaacatccc gcccgccaga tatgcggtca ttgaagtagg aacccaatca atgcccgcct
     4441 gatattgttc caccacggcc aacataccgg gttgaatcat atctgccgat ataggttgaa
     4501 aattcgtaaa gcaccagaca gagagggaaa agtaacgcct gacgtccaag cctggcaccg
     4561 gaagctaatt tattttgcat gcaatttctt cgccaataat aatcgcgcag agtttaataa
     4621 aagcgcagct aacgagaaag cgaattttgt agctgaaacc acggttaagc acattcttac
     4681 attattgcga gtatagctac gctttcttta agttttattt aacctatgcc cgttacaatc
     4741 acccaccgta aacaggccgc ttgagggaaa taagacgatg ccgctttacc cagtttaacc
     4801 tgcactttat tctcaacgac ttgcctgtat tggctccctt ttaatcactt tgcgtcggga
     4861 agttatgctg gaaaatttga atctctctct attctctctt attaacgcga cgccagactc
     4921 ggctccgtgg atgatctcgt tggcgatttt tattgctaaa gatttgatta ccgtggtgcc
     4981 gctgctggcc gcggtgcttt ggttgtgggg gcttacagca caacggcaac tggtgataaa
     5041 aatcgctatc gcgctggcgg tcagcctgtt tgtgtcctgg acgatgggac atctttttcc
     5101 gcacgaccga ccctttgtcg aaaatatcgg ctataacttc ctgcatcatg cggcggatga
     5161 ctcattccca agcgaccacg gtacggtgat tttcaccttt gcactggcat ttttatgctg
     5221 gcatcgcctg tggtccggct cacttttaat ggtgctggcc gtcgtcattg cctggtcgcg
     5281 cgtttatctt ggcgtccact ggccgctgga tatgctcggt ggattgctgg caggtatgat
     5341 tggctgcctt agtgcccaga ttatctggca agcgatgggg cataaactct atcaacgtct
     5401 gcaatcgtgg tatcgcttct gttttgcatt accgatccgc aaaggctggg tgcgtgactg
     5461 atctctgcta aaaagtgtag tattgagcgg ctcgcttcaa taactattca gagggattat
     5521 ggaaacacgt cgcgaagagc gtatcgggca gctgctgcaa gaattaaaac gcagcgataa
     5581 gttacatctt aaagacgccg ccgccctgct tggggtttcg gagatgacga ttcgtcgcga
     5641 tctgaacaac cacagtgcgc ccgtcgtttt gctcggcggc tatattgttc tggaaccgca
     5701 tagtgccagc cattacctgt taagcgatca aaaatcccgc ctggtggaag aaaaacgccg
     5761 ggcggcaaaa ctggctgcga cactggtaga acccgatcag accctctttt ttgactgtgg
     5821 caccaccacg ccgtggatta ttgaagcgat tgataatgaa atccctttta ccgccgtttg
     5881 ttattcgcta aatacctttc tggcgctgaa agagaaaccc cattgccgcg catttctgtg
     5941 cggtggtgaa tttcacgcca gcaacgccat tttcaaaccc atcgattttc agcaaacgtt
     6001 gaataatttt tgcccggata tcgcttttta ttccgcggcg ggcgtgcatg tcagtaaagg
     6061 cgctacctgt tttaatcttg aagagttgcc ggtaaaacac tgggccatgt cgatggcgca
     6121 aaagcatgtg ctggttgtcg accacagtaa atttggcaag gtccgtccgg cgcgcatggg
     6181 tgacctgaaa cgctttgata ttgtggtgag tgattgttgc ccggaagatg agtatgtgaa
     6241 gtacgcgcag acgcaccgta ttaagttgat gtattaatga cgtataaccg gatgacgttt
     6301 cgcgccatcc ggttatcaga agattaagag aaccagctgc cgaaccactg atggaatttc
     6361 atcatcacga aatcccacat cctaccaaag aatccgccct cttccacatt ttccatcacg
     6421 atcagcggac gctgctcaat ggatttaccg ttaagctgga aatcaatggt cccgacaacc
     6481 tgaccttttt tcagcggtgc ggtaagctgc ggttccgtta acgtgtagct cgccttcagg
     6541 tttttcagct gcccacgcgg tatggtcact gagcccgctt cgcctgcccc gagattcact
     6601 tcgctcttat caccaaacca gacgcgctga gtcacaaagg tggcatcagg tttaattggc
     6661 gtcacggttt caaagaagcg gaaaccccag gtcaataatt tctcagactc atcggtcagt
     6721 tttcgccccc aacactacgg agattaaacg catatcgccc tgggtagccg aagcaaccag
     6781 attatatccc gcgcctgccg tggttcctgt cttcatgcca tcaacattca gattgctgct
     6841 ccacagcaga cggttacggt taggctgacg aattttgttg aaggtgaact ctttctcttt
     6901 atgaatggcg tactcttccg gcacatcgtg gatcaatgct ttacccagca atgccatatc
     6961 gcgcgcggta ctgaactgcc ccggcgcatc caggccgtgc accgtctgga aggtagtgtt
     7021 ggtcagaccc agttttttgg cataaccatt catcagacca ataaatgact cctggctccc
     7081 ggcaacgtaa tcggccagcg caatacaggc gtcattaccg gactggataa tcacatcttt
     7141 gttcaagtct gccaccgaaa cctgatcgcc cggtttgagg aacattaccg atgaaccacg
     7201 cagtgccgga tttcccgtcg cccaggcatc tttaccgacc gtcaccatat cggtgagttt
     7261 aatcttatcg gccttaagcg cctgcccaac cacatagctg gtcatgattt tagtcaggct
     7321 cgcgggatcc agtttctcat ccgcattgcc ttctgccagc actttaccgc tggcgtaatc
     7381 cattaaaatc catgcacgcg catccacgct cggcgcttca acggtttgtt cggccgcgaa
     7441 tgccgttggg gcaaaaagga ataaaaatgc agaacccgct gcaagaccac gaaggagaga
     7501 ggagtattgc gtcataaatg ccacccgagt atccattcaa aaaaaatacg ttatatcgcc
     7561 gtatgataag ttcggtgatt aataacgcac acacaaactt aaagaaaccg taagttggta
     7621 aagtttttaa agtttatgca atatcctgcc gcttcgctgc ttctggtgtc atttttttgc
     7681 cagctgttgc ttaaaagtta actttacttc accatcggag tcctgttcag cctgtggctg
     7741 cctgtcggaa aaggggtgag tatgattacg ctgtggggtc ggaataattc aaccaacgta
     7801 aaaaagtatt gctgacgctc gaagaactgg aactacctta tgagcaaatt ctcgcgggcc
     7861 gtgagtttgg gataaatcac gatgctgatt ttctggcgat gaaccctaac gggctggtgc
     7921 cattgttgcg tgacgacgaa agtgatctca ttctttggga atcaaacgcc attgtccgct
     7981 atctggcggc acagtacggg caaaaacgcc tgtggatcga ctcaccggca cgtcgtgcgg
     8041 aagcggaaaa atggatggac tgggcaaacc agacgctcag taatgcccat cgagggatcc
     8101 tgatgggatt agtcagaaca ccaccggaag agcgcgatca ggccgccatt gatgccagtt
     8161 gcaaagagtg cgacgccctg tttgccctgc tcgatgcgga actggcaaaa gtaaaatggt
     8221 tctctggcga cgagtttggt gtgggcgata tcgctatcgc accgttcatc tacaatttgt
     8281 ttaacgttgg cctgacctgg acaccgcgtc caaatctgca acgctggtat cagcaactca
     8341 ctgaacgccc cgcagtgcgc aaagtggtga tgattcccgt tagctaattg cgtgggctaa
     8401 ctttaagtaa ttccccactg gactcgtcgg tgagaacgta taaataaccg tcgggtccag
     8461 tgcgaacatc acgaattcgc tgccctctgt ccgttaaaat acggccatct tctgtcactt
     8521 tgtcgccgtt gacgctcatc acaatgacat ctttatcttt cagcgcgcca ataaataatt
     8581 tttgctgcca ctgggggaat ttatcgctgt tatagaaggc catgccgctc acagcgggcg
     8641 aatctttcca gtaaaaaaca ggttgctcgg tcccggcgac gatctcccct ttcgcttccg
     8701 gtatcttaaa gcctgaatag ttgattcccc aggttgccag cggccagccg tagtttttgc
     8761 ctttttgcgg gatattaatt tcatcaccac cgcgcgggcc atgttcattc agccacagtg
     8821 cattactcca cggattcatc gccattcctt gcggattacg aatgccataa gaccagatct
     8881 cggcgcgcgc accggattcc tttataaaag gattatcatc cgggatttcg ccctgatcgg
     8941 tcagacgcac cagtttgccc tgtaatttat ccagatcctg cgccgtcggg cgctgattgt
     9001 tttcgcccag agcaataaaa agataacctt taccgtcgaa taccagccgc ccgccaaaat
     9061 ggttgccggt agacagtttt ggcatctggc ggaagacggt gcggaagtcg gtcacttttg
     9121 agagatcatc acttaagcgg ccataaccca cagcagttcc ggctttgcca tcatcgccaa
     9181 cttcggaata acttaaccag atgcggcgag actgagcaaa atcaggcgct aaaaccacgt
     9241 ccagcaggcc gccctgcccg tgcgcccaaa cgtccggaac tccggaaagc ggcgcagata
     9301 atccttttcc tgcttgccag tgacgcaact cgccgccgcg cagagtgatt aacataccgt
     9361 gattatcggg taaaaaggcc agtgcccagg gatggtcgag tttgtcttgc agtacttcga
     9421 catttaccgt tgcaggagcc gcccagagag cggaagaaag acaaataagg ggcacaagga
     9481 aaaaggat
//
LOCUS       DAJVUJ010000161         9090 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.161, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000161 DAJVUJ010000000
VERSION     DAJVUJ010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9090
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.161"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            52..936
                     /locus_tag="OQQ48_003505"
     CDS             52..936
                     /locus_tag="OQQ48_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HCS1954143.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFCPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSISMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(987..1841)
                     /gene="yeaE"
                     /locus_tag="OQQ48_003506"
     CDS             complement(987..1841)
                     /gene="yeaE"
                     /locus_tag="OQQ48_003506"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HCS1954144.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLRDNVFLVSKVYPWNAGRQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISSAQVLLARVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELTMLDKAYPAPK
                     GKTALDMV"
     gene            complement(1931..2677)
                     /gene="mipA"
                     /locus_tag="OQQ48_003507"
     CDS             complement(1931..2677)
                     /gene="mipA"
                     /locus_tag="OQQ48_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HCS1954145.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            3113..5047
                     /gene="yeaG"
                     /locus_tag="OQQ48_003508"
     CDS             3113..5047
                     /gene="yeaG"
                     /locus_tag="OQQ48_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HCS1954146.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            5160..6443
                     /locus_tag="OQQ48_003509"
     CDS             5160..6443
                     /locus_tag="OQQ48_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HCS1954147.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAMNKRSVT
                     DVDSGESISIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            6590..8066
                     /locus_tag="OQQ48_003510"
                     /pseudo
     CDS             6590..8066
                     /locus_tag="OQQ48_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
     gene            8160..8246
                     /locus_tag="OQQ48_003511"
                     /pseudo
     CDS             8160..8246
                     /locus_tag="OQQ48_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459804.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            8247..>9090
                     /locus_tag="OQQ48_003512"
     CDS             8247..>9090
                     /locus_tag="OQQ48_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCS1954148.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGREVAK
                     FSVPL"
BASE COUNT         2392 a         2181 c         2108 g         2409 t
ORIGIN      
        1 cttcggtcgc tctttttttt acctgataaa atgaagttaa aggactgcgt catgattaag
       61 aaaatttttg cccttccggt catcgaacaa atctcccctg tcctctcccg tcgtaaactg
      121 gatgaactgg acctcattgt ggtcgatcat ccccaggtaa aagcctcttt tgcattacag
      181 ggcgcacacc ttctctcgtg gaaacctgcg ggtgaagaag aagttctgtg gttgagcaac
      241 aacacaccgt tcaaaaatgg cgtcgctatt cgcggtggcg taccggtttg ctggccgtgg
      301 tttggtccgg cggcacaaca aggtctgcct gcgcacggtt ttgcccgcaa cctgccgtgg
      361 acgctgaaat cacatcatga agatgctgat ggcgtagcgc tgacttttga attgacgcaa
      421 agcgaagaga cgaaaaaatt ctgtccgcac gactttacgc tgttagcgca tttccgcgtg
      481 ggtaaaacct gtgaaatcga tcttgaatca catggcgaat ttgaaaccac ctctgccctg
      541 catacctact ttaacgtggg tgatatcgct aaggtaagcg tcagtgggct gggcgatcgc
      601 ttcattgata aagtgaatga cgcgaaagaa gatgtactga ccgacggtat tcagaccttc
      661 cctgaccgta ccgatcgcgt gtatctgaat ccgcaagatt gcagcgtgat taatgatgaa
      721 gcgctgaatc gtattatcgc cgtaggccac cagcatcatc tgaacgttgt aggctggaac
      781 ccgggaccgg cgctttcaat tagcatgggc gatatgccgg atgatggcta caaaacattt
      841 gtttgtgtag aaacggctta cgcttcagaa acgcaaaaag tcaccaaaga gaaacctgca
      901 catctggcgc aatccattcg cgttgcgaaa cgttaatttg cgttaatgtt gtgtgccggg
      961 tgcgatgcat ccggcacgta tcaacatcac accatatcca gcgcagtttt tccttttggt
     1021 gccggatacg ctttatccag catggttaat tccgctgacg aaagttcgac ctcaagcaca
     1081 gccgcattct gttgaacatg ggcaatcgtg gccgcttttg gaatcgccat cacaccctga
     1141 tggctgatta ccctcgccaa caatacttgt gccgagctga tattgtgagc atgtgcaatt
     1201 tcgttgacta ccgcgttttt taacagtcca ttgcgcaacc gcccggcctg ggctaatgga
     1261 ctgtaagcca tcaccggcat ctgctgttgc tggcaccagg ggagtagatc gtactcaatc
     1321 cctcgtgaac cgagatggta aagcacctga ttagtggcac actgatttcc ccccggcagc
     1381 tgccagagtt cctgcatatc agcataatca aggttagaaa cgccccagcg gcggattttt
     1441 ccctgggcga tcaatttttc catcgctgca acagtctctt caaaagcgaa actgccagac
     1501 cagtgtaata agtagagatc gagataatca gtattgagac ggcgtaaact ggcttcgcat
     1561 gcatttatcg ctttttgcct gccagcattc cacggataga ctttagagac gagaaagacg
     1621 ttatcacgca gaccggttaa tgcttcccca accacctttt cggcaccgcc atcggcatac
     1681 atttcggcgg tatcaatgag ggttaaaccg agctcaatgc ccgcgcgtag tgcagcaact
     1741 tctgttttgc gctgactggc atcttcgccc atataccatg ttccctgccc tatggctggc
     1801 agtgagacat cgccactaaa ttgaatcatt ttttgttgca ttgtttcctc ccaggtattg
     1861 caccaccgta atgcaaaaca gggcgtgacg ccctgttttt atgcacaaaa tgccctggaa
     1921 agatgcatta tcagaatttg taggtgatcc cggtagaaat caggccagtc caggatttat
     1981 ccaccatcgg gctgtcagta acttcatcag acagacgggt atagcgcgcg gtaccgtaaa
     2041 cactccagtc gccgaggaag ttgtagctgg cgctcagctc caggtaaggg ctccagctgt
     2101 cgttcgggtt atagccacgc agaccgctgc gagcggactc tttgcgcgat acgccataat
     2161 agtattcgtt ctggttttcg ctgttccact gcacaccaat acccggagtc acggtcaggc
     2221 caccgttggt gtaacgatac aaccaggcca tatcccagac aatgccgttg ctgttatcca
     2281 gggtatcgcc agccagggtg gtacgcaggt aaccgtactg ggtaaagtga gcataagaca
     2341 gaccagccat catggtgctc ttacggtcat ccaggtgacg catttggtga tcgccactgt
     2401 ctttcgcttt gaagtaaagc ggcgaccagt aagcggtaat tgaaagttta tccgttgcgt
     2461 cattccacag gtagtaacca ccacctaagc cacggaacca gaagttatcg ccttcatagt
     2521 tgattaccgg tactgggtaa acatcggtat cgtaatcttt atatgggtgc tcaacgacac
     2581 ctacgcctgc gcccaaggaa aatttacctt cagcgtgcgc tacgcctgca gacgttgcga
     2641 taagcactcc aagtgccaga agtttgagtt tggtcacaat taatcattcc ttaaacaaat
     2701 gtttagcggg cgacaaagtt tacccgtcaa tacatcgaaa cccaaccttt ttacgttttc
     2761 attttttaaa gtaactgttt aattttcctg ccgcggatga caccgcgctt acagccaaat
     2821 gaatttagcg ttactggcga gcctggtctt tacattaatt atgcaaaatt tatggatgag
     2881 ttgttgatat gccattgaaa ttaagaaagc cgtgcaggca agttttccat ttgccatcta
     2941 cgcttaattt tgaaggtgta tcaccgggca cgttgttctc atcgtcgata aaatggcatg
     3001 agagttgctg tgttttagca agagacgtcg ttcagtttac ctcttccggg aggctctact
     3061 attcatatga acggctctta acctgtgcta aaaaacgaaa ggacggcata ccatgaatat
     3121 attcgatcac tatcgccagc gatatgaagc tgccaaggac gaagagttca cactgcagga
     3181 gtttcttacc acttgtcggc aagatcgcag tgcttatgcc aacgcggctg agcggctatt
     3241 gatggctatc ggtgagcctg tcatggtcga tacagcccag gaacccagac tttctcgact
     3301 cttttctaac cgggtcattg cacgttatcc ggcgtttgaa gagttttacg gcatggaaga
     3361 cgcgattgaa cagattgtct cttatctgaa acacgcggct caggggctgg aagagaagaa
     3421 acaaatcctg tatctgctgg ggcctgtggg tgggggtaaa tcatcgcttg ctgagcgact
     3481 gaaatcatta atgcagctcg taccgattta tgtattgagc gccaacggtg agcgtagccc
     3541 ggtcaacgat catccgttct gtcttttcaa tccgcaggaa gatgcgcaga ttctggaaaa
     3601 agagtatggc attcctcgcc gttatctcgg caccatcatg tcgccgtggg cggcaaaacg
     3661 cctgcatgaa tttggtggcg atatcactaa gttccgggta gtgaaggtct ggccgtcaat
     3721 tctgcaacaa attgctatcg ccaaaacgga acccggcgat gagaacaacc aggacatctc
     3781 cgcgctggtt gggaaagtcg atattcgtaa actcgaacac tacgcgcaga atgacccgga
     3841 cgcctacggc tattccggtg cgctgtgccg cgccaatcag gggatcatgg aattcgttga
     3901 gatgtttaaa gcaccgataa aagtgctgca tcccttgtta accgccactc aggaaggtaa
     3961 ctacaacggg acggaaggta tctccgccct gccgttcaac gggattattc tcgcccactc
     4021 gaacgagtcc gaatgggtca ctttccgtaa taacaaaaac aacgaagcct tcctcgaccg
     4081 tgtttacatc gtgaaggtgc cgtattgctt gcgcatttcc gaagagatca aaatctacga
     4141 gaaattgctt aatcacagtg aattgactca cgccccatgc gcccctggca cgctggaaac
     4201 actgtcacgt ttttccattc tttcgcgcct gaaagagcca gaaaactcca gcatttattc
     4261 aaagatgcgg gtttatgatg gcgaaagtct gaaagacacc gatcccaaag ccaagtcgta
     4321 tcaggaatat cgtgactacg ccggtgtcga tgaagggatg aacggtctgt cgacgcgttt
     4381 tgcgtttaag atcctctccc gcgtgttcaa cttcgatcat gtagaagtgg cggcaaaccc
     4441 ggtccatctg ttctacgtcc tggaacagca gatcgagcgc gagcagttcc cgcaagagca
     4501 ggcagaacgc tatctggagt tcctgaaagg ttatctgatc ccgaaatatg ccgagtttat
     4561 cggcaaagag atccagacgg cctaccttga atcctattcc gaatatgggc aaaacatttt
     4621 cgaccgttat gttacctacg cggatttctg gattcaggat caggagtatc gcgatccgga
     4681 taccgggcag ctgtttgacc gcgagtctct taacgccgag ctggagaaaa tcgagaaacc
     4741 ggcggggatc agtaatccaa aagatttccg caacgagatt gttaacttcg tactgcgcgc
     4801 cagagcgaat aacagcggac gcaatccgaa ctggaccagc tatgaaaaac tgcgcacggt
     4861 tatcgagaag aaaatgttct ccaataccga ggagctgttg ccggttatct cgtttaacgc
     4921 caaaacgtca accgacgagc agaagaaaca cgacgacttt gtcgaccgta tgatggaaaa
     4981 aggctacacc cgtaaacagg tgcgtttact gtgcgaatgg tatttgcgcg tacgtaaatc
     5041 gtcttaacaa ccctggcccg gtacacatgc taccgggcct acaacgacag cgaaccgtgg
     5101 gccagagaaa tggcaacaca ggcgtagcat acagttggca aatgtagtac ggggggcata
     5161 tgacctggtt tattgaccgg cgtctgaacg gcaaaaacaa aagcatggtg aatcgccagc
     5221 gttttttacg ccgttataaa gcgcaaatta aacagtcgat ctccgaggcc atgaataagc
     5281 gttcggtgac tgacgtcgac agcggcgaat ccatatccat tcccacggaa gatattagcg
     5341 aaccgatgtt tcatcagggg cgtggcggcc tgcgccaccg cgtacatccg ggcaatgacc
     5401 atttcgtcca gaacgaccga attgaacgtc cccagggtgg cggcggaggt tccggcagtg
     5461 gtcagggcca ggccagccag gatggtgaag gtcaggatga atttgtcttt cagatttcga
     5521 aagatgagta tcttgatctg ctctttgaag atttggcctt accgaatctg aaacaaaacc
     5581 aacaacgcca gctgaccgaa tataaaacgc atcgggcggg ttataccgct aacggcgttc
     5641 cagccaatat cagcgttgtg cgttccttgc agaactcact ggcgcgacgc acagccatga
     5701 cggcgggcaa gcgacgggaa cttcatgcgc tggaagagaa tttggccatc atcagcaaca
     5761 gtgaacctgc gcaactgctg gaagaggaac gtctgcgcaa agaaattgca gaattacgtg
     5821 ccaaaattga acgcgtccct tttattgaca ccttcgattt acgttacaag aactacgaga
     5881 agcggcccga tccctccagc caagcagtga tgttttgcct gatggacgtt tccggttcaa
     5941 tggatcaatc cactaaagat atggctaagc gtttttatat tctgctgtat ctgttcctca
     6001 gcagaacgta taagaacgtg gaagtcgtat acatccgcca tcatacccaa gcgaaagaag
     6061 tcgatgaaca tgagtttttc tactcgcagg aaacaggcgg caccattgtt tccagcgccc
     6121 tgaaactgat ggatgaggta gtgaaagagc gttataaccc ggcacagtgg aatatttacg
     6181 ctgcacaagc atcggacggc gataactggg ccgatgactc tccgctttgc catgaaatcc
     6241 tggcgaaaaa attattacct gttgttcgtt attacagcta tatcgaaatt acccgtcgtg
     6301 cacatcagac attgtggcga gaatatgagc atctgcaatc tactttcgac aactttgcga
     6361 tgcagcacat ccgcgaccag gatgatattt atccggtgtt ccgtgaactg tttcataaac
     6421 aaaatgcaac agctaaagac taaaactatc agccaggtca ttatcgcctg gctgattttt
     6481 agcttactgt aaattatctc atttattaca tacattagct tacaatcgct ttaaatatga
     6541 cagcataacc tttacataat ttagttccag aaaacaatca ttcggaaaaa tgattcagtc
     6601 aacacgtatt tccatggggt tattctttaa atatttttta tcgttaacga aaattgatcc
     6661 tggtcaaaac tatatatctc tgccatcaat aaaatccagc actcacattg ctctcctttt
     6721 tatggtttct atgggtacac aaaaattaaa agctcaaagc ttttttattt tcagtttatt
     6781 gctgacatta attttatttt gcattactac cttatataac gaaaacacaa atgtaaaact
     6841 catcccacag atgaattacc tgatggttgt tgtggctttg tttttcctta acgccgtcat
     6901 ttttcttttc atgttaatga aatatttcac taacaaacaa attttaccaa cactcatttt
     6961 aagccttgca ttcttaagtg gccttatcta tttagttgaa accattgtaa ttatccataa
     7021 accaattaac ggcagtacac tgatccagac aaagtcgaat gatgtttcta ttttctatat
     7081 tttccgccaa ctcagtttta tttgtttaac ctcgctggca ctcttttgtt atggaaaaga
     7141 caacatcctt gacaacaata agaaaaaaac gggaatcctg ttgctggcgt tgattccttt
     7201 tttagttttt ccccttctgg cacacaatct gagcagttat aactgctgac tattctttgt
     7261 atgtcgtcga ctactgtccg gacaaccata ctgcgaccag gggaatcaac tatacaaaaa
     7321 tattggtttg tctgtgggca tttttactgt tctttattat catgcgtaca cgattagcca
     7381 gcgaactatg gccgttaata gcattattat gtctggcatc gctatgctgc aacttacttc
     7441 tactgactct ggatgagtat aattacacca tctggtatat cagtcgcggg attgaagttt
     7501 ccagtaaact gtttgttgtg tcttttctga tttataacat ttttcaggag ctgcaactct
     7561 ccagcaaact ggcagttcat gatgtgctga ccaatattta taatcggcgc tactttttca
     7621 acagcgtaga gtcattattg tcgcgacctg ttgttaagga cttctgtgtc atgctggttg
     7681 atattaatca gttcaaacgc atcaatgccc aatggggaca tcgtgtgggt gataaagtgc
     7741 tggtttcaat tgtcgatatt atccagcaaa gcatccgccc tgatgatatt ttagcgcgac
     7801 tggagggtga ggtgtttggc ttgctattta ccgaactcaa tagtgcccag gcaaaaatca
     7861 ttgcggatcg tatacgtaaa aatgtcgaac tcctgaccgg ctttagtaac agatatgatg
     7921 ttcctgaaca aatgaccatc agtattggca cggttttttc aacgggtgac acgcgtaata
     7981 tctcgcttgt catgacggaa gcagataaag ccttacgcga agcgaaaagc gaggggggca
     8041 acaaagtgat tattcatcat atttaagtgc aaaaatattc agagccatgc tttttgcgtg
     8101 gcttttgcat acaatttatt actattaccc ctaaatttcc cctccccagc ggcgcagaga
     8161 tgagtataat tagcgcccct gtgccaggcc gcaatcgaac tttatctggt tttctcgttt
     8221 cactaactga aggagtgcca tttatcatga aattgcacca tagaatgctc cggcatttta
     8281 tcgccgcaag tgtcattgtg ctgacatctt ccttccttat ttttgaactt gtcgccagcg
     8341 acagagcaat gagtgcctat ctgcgctata tcgtgcagaa agcagactcc tcctttcttt
     8401 atgataagta tcagaatcag agtattgccg cgcatgtgat gcgcgctctc gctgctgagc
     8461 agtcggaagt gtcgccagaa cagcggcgcg ccatctgcga ggcttttgag tctgccaata
     8521 atacccatgg cttaaacctg actgctcata aatacccggg cttacgcggc acactacaaa
     8581 ccgcatccac tgactgcgac acaattgtgg aagctgcagc actattaccc gcttttgatc
     8641 aggcagtgga aggcaaccgc caccaggatg attacggttc aggtcttggg atggccgaag
     8701 agaaatttca ctattatctc gatctcaatg accgctatgt ctatttttat gagccggtta
     8761 atgttgaata ctttgcgatg aataactggt ccttcctgca gtcaggaagt attggtatcg
     8821 atcgcaaaga tattgaaaag gtatttaccg ggcgtaccgt attgtcgagc atttaccagg
     8881 atcagcgtac taaacagaac gtgatgagtt tgctgacgcc ggtgtatgtt gcagggcagc
     8941 taaaagggat tgtgctgctg gatattaaca aaaacaattt gcggaatatc ttttacactc
     9001 atgaccgccc tctcctctgg cgttttctca atgtcacgct aaccgatacc gattcggggc
     9061 gtgaagtggc caaattcagt gtgccacttc
//
LOCUS       DAJVUJ010000162         9088 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.162, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000162 DAJVUJ010000000
VERSION     DAJVUJ010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9088
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.162"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..910
                     /locus_tag="OQQ48_003513"
     CDS             <1..910
                     /locus_tag="OQQ48_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HCS1954149.1"
                     /translation="GGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDH
                     VMHELDPALDLRNVGVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINR
                     AYEEGWVGNHALAFIGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPA
                     PPRGVVQRRLTFTHHRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQG
                     GEPRQLTHNKTDIQSAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTESHANPPSAD
                     AVVFSPDGQWLAWMEGGQLWITETDR"
     gene            complement(912..1244)
                     /locus_tag="OQQ48_003514"
     CDS             complement(912..1244)
                     /locus_tag="OQQ48_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HCS1954150.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSHVEKSEANAQATNAVI
                     PPARMPDN"
     misc_feature    1484..1556
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            1550..1963
                     /gene="ibpA"
                     /locus_tag="OQQ48_003515"
     CDS             1550..1963
                     /gene="ibpA"
                     /locus_tag="OQQ48_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HCS1954151.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     gene            2075..2503
                     /gene="ibpB"
                     /locus_tag="OQQ48_003516"
     CDS             2075..2503
                     /gene="ibpB"
                     /locus_tag="OQQ48_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HCS1954152.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            2699..4360
                     /locus_tag="OQQ48_003517"
     CDS             2699..4360
                     /locus_tag="OQQ48_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HCS1954153.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAPALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(4357..5073)
                     /locus_tag="OQQ48_003518"
     CDS             complement(4357..5073)
                     /locus_tag="OQQ48_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCS1954154.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            5368..6984
                     /locus_tag="OQQ48_003519"
                     /pseudo
     CDS             5368..6984
                     /locus_tag="OQQ48_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
     gene            6984..8307
                     /locus_tag="OQQ48_003520"
                     /pseudo
     CDS             6984..8307
                     /locus_tag="OQQ48_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
     gene            complement(8438..8869)
                     /gene="tnpA"
                     /locus_tag="OQQ48_003521"
                     /pseudo
     CDS             complement(8438..8869)
                     /gene="tnpA"
                     /locus_tag="OQQ48_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            8877..>9088
                     /locus_tag="OQQ48_003522"
     CDS             8877..>9088
                     /locus_tag="OQQ48_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS1954155.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALARQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWL"
BASE COUNT         2111 a         2268 c         2396 g         2313 t
ORIGIN      
        1 aggcggcaag gtgagcaatc tcgatgcaat ggatattacc gcgccgtata cgccaggagc
       61 gctgcgcggc ggcagccatg tgcatgtctt tagcccgaac ggtgaaaggg tgagctttac
      121 ctataacgac catgtaatgc atgaactcga tccggcgctg gatttgcgaa acgtcggtgt
      181 tgctgcgccg tttggcccgg tcaacgtaca aaagcagcat ccgcgtgaat acagcggtag
      241 ccactggtgc gtgctggtga gtaaaaccac gcccacgccg cagcctggca gcgatgaaat
      301 caatcgtgct tatgaagaag gatgggtagg aaatcacgcg ctggcgttta ttggcgacac
      361 actttcgcca aagggcgaga aagtgccgga gctgtttatc gttgagttac cgcaagatga
      421 agctggctgg aaagcggcag gtgatgcgcc gttaagtgga acggaaacca ccctgcccgc
      481 gccaccgcgc ggcgtcgtgc agcgacgttt aacctttacc caccatcggg cttatccggg
      541 gttagtcaac gtcccgcgcc actgggtgcg ctgtaatccg cagggtacgc aaatcgcgtt
      601 tttaatgcgt gatgataacg gcattgtgca actgtggctt atctcgccac agggcggcga
      661 gccgcgccag ttaacccata acaaaacgga tattcagtct gcatttaact ggcatccgtc
      721 aggagaatgg ttgggctttg tgctggataa tcgaattgct tgtgcccatg cgcaaagtgg
      781 cgaggttgag tatttaaccg aaagccacgc caatccacct tctgcggacg ccgtggtctt
      841 ctcgccggat ggtcaatggc tggcgtggat ggaaggcggc cagctgtgga tcaccgaaac
      901 tgatcgctaa atcagttatc aggcattctg gccggtggaa taaccgcatt ggtggcttgc
      961 gcgttggctt cgcttttttc aacgtgtgag cgcaccgagc tgtctttgcg gaacacatcc
     1021 cacggcagta aaagcgtgtc catcacagcg gtaaacggca tatcgagaat cgccagcgac
     1081 ttagtgcccc agttggtttc atcgtcgcca atcatggttg cgctggcgcg ggtgccggga
     1141 tacgttcctt ctttccctcc ggtatgggac atcacgctgg agcatcctcc gagtaatgtc
     1201 attccgctgc atatcatgaa cgctaacaac acatttctta tcataataaa ttcatctgtt
     1261 gatcgtgggt gttggcctga tgagttatag cgatcccttg ctgaaaataa catcatcatt
     1321 acgtcgcact gtggcggcta tcgcacttta acgtttcgtg ctgccccctc agtctatgca
     1381 atagaccata aactgcaaaa aaaagtccgc tgataaggct tgaaaagttc atttccagac
     1441 ccatttttac atcgtagccg atgaggacgc gcctgatggg tgttctggct acctgacctg
     1501 tccattgtgg aaggtattac attctcgctg atttcaggag ctattgatta tgcgtaactt
     1561 tgatttatcc ccgctttacc gttctgctat tggatttgac cgtttgttta accacttaga
     1621 aaacaaccag agccagagta atggcggcta ccctccgtat aacgttgaac tggtagacga
     1681 aaaccattac cgcattgcta tcgctgtggc tggttttgct gagagcgaac tggaaattac
     1741 cgcccaggat aatctgctgg tggtgaaagg tgctcacgcc gacgaacaaa aagagcgcac
     1801 ctatctgtac cagggcatcg ctgaacgcaa ctttgaacgc aaattccagt tagctgagaa
     1861 cattcatgtt cgtggggcta acctggtaaa tggtttgctg tatatcgatc tcgaacgcgt
     1921 gattccggaa gcgaaaaaac cgcgccgtat cgaaatcaac taattcccta aggccgcctg
     1981 acgcggcctg acatctccat gctcgccgtc agggagcata tgcgaatctt cggatttgca
     2041 ggtacttact cgcttcttag aaggagaaat gactatgcgt aacttcgatt tatccccact
     2101 gatgcgtcaa tggatcggtt ttgacaaact ggccaacgca ctgcaaaacg ccggtgaaag
     2161 ccagagcttc ccgccgtaca acattgagaa aagcgacgat aaccactacc gcattaccct
     2221 tgcgctggca ggtttccgtc aggaagattt agagattcaa ctggaaggta cgcgcctgag
     2281 cgtaaaaggc acgccggagc agccaaaaga agagaaaaaa tggctgcatc aagggcttat
     2341 gaatcagcca tttagcctga gctttacgct ggctgaaaat atggaagtct ctggcgcaac
     2401 cttcgtaaac ggtttactgc atattgattt aattcgtaat gagcctgaac ccatcgcagc
     2461 gcagcgtatc gctatcagcg aacgtcccgc gttaaatagc taactagcta ttctctttgc
     2521 cccgccattc tggtggggct ttttttgtct ttcttcttca acaatgcgtt aaatttcgac
     2581 tgtttaagat atttcggcac gttctttccc gtgataatgt gcgctataag gcaaatctgc
     2641 ttcaccgatt cagccataat ccgtagcaat taaaatgtta tctcaagaga aatggacgat
     2701 gagtgatata gcattaacgg tcagtattct ggctttggtg gcagtcgtcg gtttgtttat
     2761 cggcaacgtc aaatttcgcg gcataggatt aggtattggc ggcgtgctgt ttggtgggat
     2821 catcgtcggc cattttgttt ctcaggcggg gatgacatta agtagcgata tgctgcatgt
     2881 tattcaggaa tttggcctga tcctgttcgt ttataccatc gggattcaag tagggccggg
     2941 cttctttgcc tcattgcgcg tctccggatt acgcctcaac ctgtttgctg ttctgatcgt
     3001 catcatcggt ggtctggtta ccgccatcct gcataaactg tttgatattc cactgccggt
     3061 agtgctgggg attttctccg gtgcggttac caatacgcca gcgctggggg cagggcagca
     3121 gatcttgcgc gacctgggta caccaatgga aatggtcgat cagatgggga tgagttacgc
     3181 gatggcgtat ccattcggca tttgcgggat tttgttcacc atgtggatgt tgcgggttat
     3241 tttccgcgtc aatgtcgaga cagaagctca gcagcacgag tcttcacgca ccaatggcgg
     3301 cgcgctgatc aagactatca atattcgcgt tgagaaccct aacctgcatg atttagctat
     3361 taaagatgta ccgattctca acggcgacaa aattatctgc tcgcgtctga aacgcgaaga
     3421 aaccctaaaa gttccttcgc cagataccat tatccaactg ggcgatttgc tgcatctggt
     3481 ggggcagcca gcggatttac ataatgcgca actggtgatt ggtcaggagg tcgatacttc
     3541 gctgtccacg aaaggcactg atttgcgcgt cgagcgtgtg gtggtcacca atgaaaacgt
     3601 gctcggaaaa cgtattcgcg acctgcactt taaagaacgc tatgacgttg ttatctcgcg
     3661 cctgaaccgt gccggggtcg aactggtcgc cagtggcgat atcagcctgc agttcggcga
     3721 tatcctcaat ctggtggggc gtccgtccgc aattgatgcc gttgccaatg tgctggggaa
     3781 tgcgcagcaa aaactgcaac aggttcagat gctgccagtg tttattggca tcgggcttgg
     3841 cgtattgtta ggttctattc ccgtctttgt gcctgggttc ccggccgcgt tgaaactggg
     3901 gctggcgggc ggtccgctga ttatggcgtt gatcctcggg cgtatcggca gtatcggcaa
     3961 gctgtactgg tttatgccgc caagtgccaa cctcgcgctg cgggagctgg ggatcgtgct
     4021 gttcctctcg gtcgttggtc tgaaatctgg tggggatttt gtgaataccc tggtcaatgg
     4081 cgaagggcta agctggattg gttatggtgc cctgatcacc gccgttccgc tgattactgt
     4141 tggcattctg gcgcggatgt tagccaaaat gaattacctg accatgtgcg ggatgctggc
     4201 aggttccatg accgatcctc cggcgctggc gtttgctaat aatcttcatc caaccagtgg
     4261 tgcgccggcg ctctcttacg ccactgtcta tccgttagtg atgtttctgc gaattatcac
     4321 cccccaatta ctggcggtgc tcttctggag tatcggttaa ctcttttccg ggtggagtcg
     4381 ccgtaaatgg tactccacct ggtattcgct ggcatttctg aacatcactg aataattcaa
     4441 aaactccccg ctctcgctat atgacaacga ggtgatccgc agcagtggcg tgtgttccgc
     4501 taccttcatt tgccgcgcca gcagtctatc ggcgagcacc ggcgtcaggc tttcataatt
     4561 accgccaatc aaaatcccac actcttgttc aatatattca aacttcgacc cttccagatg
     4621 ttgcagcgaa agattacgga acagtttaac cggcatatag ctgtcttcca gcatcagcgg
     4681 tttcccttcc acaaaacgaa cgcgacggga gaagtagatc tgctcgttga tttgaatccg
     4741 taactggctg gcaatggccg gaggcgcagg catgatttca aaaatcagca cctggctggt
     4801 gaccgttttt ccctgccgtt ttaacacctc caccagtccg gtcaggctgg cggtttgatg
     4861 cagcacatct ttgcgcacca ggtaagtgcc gctgccgtgg cggcggacca ccagccccca
     4921 cgctaccagc aggtcaatcg ctttacggat ggtcatccgg gatactgcaa attcttccgc
     4981 caactttttt tcaccgggaa gaaggctgtt tagcgtgaaa tccgcggaat taagtcgaat
     5041 tcttaaccgc tccgcaatgc ttttgtagat catctctaga tctctttttt gcctgcaagc
     5101 ccgggtaatg cgcccacaag ttctgcttaa tcgattgaaa aaacatcttt ttttaaagat
     5161 gtgttcgatg ctgtgtacca gtgctcacag atgtctactt tttcgcgaaa acgtagatct
     5221 ctaccgccca acgaaaagca tgaaagcgat cacgaatccc attgggtcgt catgttctgc
     5281 cgatcgcatc ttcctatcct cgctccaggc ctgccgcata accaatcagg cttcctactt
     5341 acagaattga gaaaagagga tgtggaaatg ctcagtcaaa ttcaacgctt tggcggcgcg
     5401 atgttcacgc cagtgctgct gtttcccttc gccgggattg tggtgggtct tgccatcttg
     5461 ctgcaaaacc cgatgtttgt cggggaatca ctgaccgatc cgaacagttt attcgcgcaa
     5521 atcgtacaca ttattgaaga gggcggttgg acggtattcc gtaatatgcc gctgattttt
     5581 gctgtcggtt tacccattgg ccttgctaag caagcgcagg ggcgtgcttg tctggcggtg
     5641 atggtgagtt tcctgacctg gaactatttc atcaacgcga tgggaatgat ctggggaagc
     5701 tacttcggcg tcgatttcac tcaggacgcg gtggcaggta gcggtctgac aatgatggcc
     5761 gggattaaaa ccctcgatac cagcattatc ggcgcaatta tcatttccgg cattgtgacg
     5821 gcgctgcata accgtctgtt cgataaaaaa ctgccggttt ttctcggcat tttccagggg
     5881 acgtcttatg tggtgattat cgccttcctg gtgatgatcc cctgtgccta gctgacgttg
     5941 ctcggttggc caaaagtaca aatggggatt gaatctctgc aagcgttcct gcgttcggcg
     6001 ggtgcgcttg gggggtgggt ttacaccttc ctcgaacgta ttctgatccc aaccggttta
     6061 caccacttca tctacggaca gtttatcttt ggtccggcag ctgttgaagg cggcattcag
     6121 atgtactggg cgcagcatct gcaagagttc agtctgagcg ccgagccgct gaaatcgttg
     6181 ttcccggaag gcggttttgc cctgcacggt aactcaaaaa tctttggtgc cgtgggcatt
     6241 tctttagcga tgtacttcac tgccgcaccg gaaaatcggg taaaagtggc gggcttgctg
     6301 attcccgcaa ccttaaccgc catgctggtg ggaattaccg aaccgctgga atttaccttc
     6361 ctgttcattt caccgttgct gtttgcggta cacgccttgc tggcggcctc aatgtcgacc
     6421 gtgatgtatc tctttggtgt ggtgggcaac atgggcggag gtctgattga ccaggtttta
     6481 ccgcaaaact ggatcccgat gttcagcaac cacgcggata tgatgctgac ccaaatcgcc
     6541 attgggttgt gctttaccct gctgtacttc gtggttttcc gcacactgat tctgcaattc
     6601 aacatgtgca cgccgggacg tgaagatgcg gaagtgaaac tctactcaaa agccgaatac
     6661 aaagcctcgc gaggccaaac caccgctgca gagccaaaaa aagagctgga tcaggctgcc
     6721 ggtatcctgc aagccctggg cggggtcggc aatatctcca gcattaacaa ttgcgcgacg
     6781 cgtttacgta ttgcactgca tgacatgtca caaacgctgg atgacgaagt ctttaaaaag
     6841 ctgggagcgc acggcgtctt ccgtagtggc gatgccattc aggtgatcat tggtctgcat
     6901 gtatcccagc tgcgtgaaca gctcgatagc ttaattaatt ctcatcaatc agcagaaaat
     6961 gttgccatta cggaggcagt ataatgacca aattctcagt ggttgtcgca ggcggtggaa
     7021 gcacctttac gccaggcatc gtgttgatgc tcctggcgaa tcaggaccgt ttcccgcttc
     7081 gtgcactgaa attttatgat aacgatggtg cgcggcagga agtgattgcc gaagcctgta
     7141 aagtcatcct taaagagaaa gcgccggaca ttgcgtttag ttacaccacc gatcctgaag
     7201 tggcattcag cgacgttgat tttgtcatgg cgcacatccg cgtcggcaaa tacccgatgc
     7261 gcgaactgga tgaaaaaatc ccgctgcgcc acggcgttgt tggtcaggaa acttgcggac
     7321 ccggcggaat agcgtacggc atgcgttcca ttggcggcgt cctggaactg gtggattata
     7381 tggaaaaata ttcaccaaat gcctggatgc tcaactactc caacccggca gcaattgtcg
     7441 cagaagccac gcgtcgtctg cgcccgaatg cgaaaatcct caacatctgt gacatgccaa
     7501 tcggtattga aagccggatg gcgcaaattg ttgggctgca agatcgcaaa cagatgcgcg
     7561 tgcgctacta cggcctgaac cactttggct ggtggacatc aattgaagat ttgcagggca
     7621 acgacctgat gccccagctg cggcaatatg tctctaagca cggttatgtt ccaccgcagc
     7681 aagatacaca tactgaagcg agctggaacg acacctatgc aaaagcgcgg gatgtccagg
     7741 cactggaccc ggatacattg ccgaacacct atctgaaata ttatctcttc ccggattatg
     7801 ttgttcagca ttccaaccct gaacataccc gcgcgaatga ggtgaatgga acatcgcgag
     7861 aaacaggttt tcgatgcttg ccgcgccatt acagcggcag gaaattcagc ggcggccaag
     7921 ctggagattg acgaacatgc gtcatacatc gtcgatctgg cggcggcaat tgccttcaac
     7981 actcaggagc ggatgttgct gattgtgcct aacaacgggg caatccataa ctttgatgat
     8041 gaagcgatgg tcgagatccc gtgtctggtc gggcacaacg gaccagaacc actggtggtc
     8101 ggcgatatcc cgcagtttca gaaagggtta atgagtcagc aagtggcggt ggaaaaactg
     8161 gtcgtggacg cctgggaaca gcgttcatat cagcacctgt ggcaggcgat tacgttatcg
     8221 aaaacggtac cgagcgcctc ggtcgccaaa gctattctgg atgagttgct ggaggccaac
     8281 aaagcgtact ggccagagtt acgttaattg tgtggactgg catccgttgg gtgctggtcc
     8341 gtattcttta gcacggctga cggcgcataa ccaaatttat ccggtgcgcc gtaaaacccc
     8401 gtccttcagg gcggggatat aaggcgcggt tttccaccta accaggtgtt tgctgttgtt
     8461 caatgtattg gcggatgacg gatattggcg cacctccgca gctattggca aaatagccag
     8521 gactccacaa aacgcctttg tagtaatacc tgactgcaat atctggtcga tctcgtcgca
     8581 gtaacctacc cgatacgcct ttgaggctgt ttaccagact ggatatcgcc agtttgggag
     8641 gatagttaat taacaaatgg acgtgatctg gttcgccatc catttaaacc agttcagctt
     8701 caaaatcagc acatacattt gaaaagtaag tgcgtagttt ttctgtcgcg tcatagtcaa
     8761 aaatctgacg tcggtatttg gtgacaaaga ccaggtgaac atgcatcagg aaaacacaat
     8821 gccttccacg ccggatatcg gtttcttttt tcacagacca aagtatcatt tggcctgtga
     8881 aacgactaca agcatttaaa ttccagttaa gacccggtgg tcaacaggag cgtgaaatga
     8941 ggcgcttcgc aggcgcatgt cgtttcgttt tcaatcgtgc tctggcacgt cagaatgaga
     9001 atcatgaggc cggtaataaa tacatccctt acgggaaaat ggcttcctgg ctggttgagt
     9061 ggaaaaatgc cactgaaacg caatggct
//
LOCUS       DAJVUJ010000163         9067 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.163, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000163 DAJVUJ010000000
VERSION     DAJVUJ010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9067
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.163"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            247..768
                     /gene="fecI"
                     /locus_tag="OQQ48_003523"
     CDS             247..768
                     /gene="fecI"
                     /locus_tag="OQQ48_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HCS1954156.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            765..1718
                     /gene="fecR"
                     /locus_tag="OQQ48_003524"
     CDS             765..1718
                     /gene="fecR"
                     /locus_tag="OQQ48_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fec operon regulator FecR"
                     /protein_id="HCS1954157.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADVRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            1805..4129
                     /gene="fecA"
                     /locus_tag="OQQ48_003525"
     CDS             1805..4129
                     /gene="fecA"
                     /locus_tag="OQQ48_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate transport protein FecA"
                     /protein_id="HCS1954158.1"
                     /translation="MTPLRVFRKTTPLVNTIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLSPRLASRSTVLMDGIPVPFAPYGQPQLSLAPVS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYLNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEIGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWTF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVAYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGSLKF"
     gene            4174..5076
                     /gene="fecB"
                     /locus_tag="OQQ48_003526"
     CDS             4174..5076
                     /gene="fecB"
                     /locus_tag="OQQ48_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HCS1954159.1"
                     /translation="MLAFIRFLFAGLLLVISHAFAAMVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIAPVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMVGASMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            5073..6071
                     /gene="fecC"
                     /locus_tag="OQQ48_003527"
     CDS             5073..6071
                     /gene="fecC"
                     /locus_tag="OQQ48_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HCS1954160.1"
                     /translation="MTAIKHPVLLWGLPIAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            6068..7024
                     /gene="fecD"
                     /locus_tag="OQQ48_003528"
     CDS             6068..7024
                     /gene="fecD"
                     /locus_tag="OQQ48_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HCS1954161.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPHTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            7025..7792
                     /gene="fecE"
                     /locus_tag="OQQ48_003529"
     CDS             7025..7792
                     /gene="fecE"
                     /locus_tag="OQQ48_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HCS1954162.1"
                     /translation="MTLRTENLTVSYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(8351..8608)
                     /locus_tag="OQQ48_003530"
     CDS             complement(8351..8608)
                     /locus_tag="OQQ48_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1954163.1"
                     /translation="MTLVNDTGFDPVFSGSIAESWRQQPCTPSYCCDWEAATMLRAFP
                     LAKKGEGRARLPSLYASFGKLGETPTHEDIIDNNRSINWPV"
     gene            complement(8691..9067)
                     /locus_tag="OQQ48_003531"
                     /pseudo
     CDS             complement(8691..9067)
                     /locus_tag="OQQ48_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611482.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
BASE COUNT         1968 a         2591 c         2501 g         2007 t
ORIGIN      
        1 cagcattacc gaaatcccca tcctttaggg cagtgaggat gtcaataata gcagaccaca
       61 tcatgagtca ttctgaccga cacatgccag aagcagatgc tccatcccat tttataccta
      121 ccttataaca cttagaaaaa caacatgtta aaaatgtcta ttggaaacaa ttttatttcc
      181 aattgtaatg ataaccattc tcatattaat atggctacgt gataattaac ttttgatgca
      241 ctccgcatgt ctgaccgcgc cactaccaca gcttccttaa cgttcgagtc gctttatggc
      301 acacatcacg gctggttgaa aagctggctg acgcgcaaac tccagtctgc ttttgatgca
      361 gatgacattg cccaggacac ttttttgcgg gtaatggtca gcgaaacgct ctcgacgatc
      421 cgcgatcctc gctccttcct gtgcactatc gccaaacgcg tgatggtgga cctgtttcgc
      481 cgaaacgcgc tggaaaaagc gtatctggag atgctggcgc ttatgccgga ggggggagcg
      541 ccttcacctg aggaacgcga aagccaactc gagaccctac aactcctcga cagcatgctg
      601 gacgggctaa acggcaaaac acgtgaagcg tttctgcttt cgcaactgga tggtctgaca
      661 tacagcgaga ttgcgcacaa actcggtgtt tccatcagct ccgtgaaaaa atacgtggcg
      721 aaagccgtcg agcactgcct gctgttccgt ctggagtatg ggttatgaat cctttgttaa
      781 ccgattcccg ccgtcaggcg ctgcgttcag cttcccactg gtatgccgtt ctaagcggcg
      841 agcgcgtcag cccacaacag gaagcgcgct ggcaacagtg gtatgaacag gatcaggata
      901 accagtgggc ctggcagcag gttgaaaacc tgcgcaacca gcttggcggt gtgcctggcg
      961 acgttgccag ccgggcgttg cacgataccc gcctcacccg ccgtcacgtg atgaaaggat
     1021 tactgctgtt gctcggcgct ggcggaggct ggcagctctg gcagtcggaa accggcgaag
     1081 gtctgcgggc agattaccgc accgccaaag gcacagtcag ccgtcagcaa ctggaagatg
     1141 gctccctgct cacgctgaat acccaaagcg cggcggatgt gcgttttgat gcgcatcaac
     1201 gcaccgtccg gctctggtac ggtgaaatcg ccattactac cgcgaaagat gccctgcaac
     1261 gccccttccg tgtcctgacc cgtcagggcc agctcactgc tttagggaca gaatttaccg
     1321 tccgccagca ggataatttc acgcagcttg acgtgcagca gcacgctgtg gaagtgcttc
     1381 tcgccagtgc ccccgcgcaa aaacgcatcg tgaacgctgg tgaaagcctg cagttcagcg
     1441 cctctgagtt tggcgcagtg aaaccgctgg atgacgagag tacaagctgg acgaaggaca
     1501 tcctgagctt cagcgataaa ccgctgggtg aggtgatagc cacgctaacc cgttaccgca
     1561 acggcgtgct gcgctgcgat cccgccgttg ccgggctgcg cctgagcggg acgttcccgc
     1621 tgaaaaatac cgatgcgatc ctgaacgtta tcgcgcaaac gcttcccgtt aaaattcagt
     1681 ctattacgcg gtactggata aacatttcac cactgtaagg aaaataattc ttatttcgat
     1741 tgtccttttt acccttctcg ttcgactcat agctgaacac aacaaaaatg atgatgggga
     1801 aggtatgacg ccgttacgcg tttttcgtaa aacaacacct ttggttaaca ccattcgcct
     1861 gagcctgctg ccgctggccg gtctctcgtt ttccgctttt gctgcacagg ttaatatcgc
     1921 accgggatcg ctcgataaag cgctcaatca gtatgccgca cacagcggat ttaccctctc
     1981 ggttgacgcc agcctgacgc gcggcaagca gagcaacggc ctgcacggcg attacgacgt
     2041 cgagagcggc ctgcaacaac tgctggacgg cagcggactg caggtaaaac cgctgggaaa
     2101 taacagctgg acgctggagc ccgcgcccgc accaaaagaa gatgccctga ccgtggtcgg
     2161 cgactggctg ggtgatgcgc gtgaaaacga cgtatttgaa catgctggcg cgcgtgacgt
     2221 gatccgccgt gaggatttcg ccaaaaccgg cgcaaccacc atgcgtgagg tacttaaccg
     2281 catccctggc gtcagcgcgc cggaaaacaa cggcaccggc agccacgacc tggcgatgaa
     2341 ctttggcatc cggggcctga gcccgcgcct cgccagccgc tcgaccgtcc tgatggacgg
     2401 catccccgtc cccttcgccc cttacggtca gccgcagctt tcactggctc ccgtttcgct
     2461 cggcaacatg gatgccattg acgtggtacg cggtggtggt gcggtgcgtt acggaccgca
     2521 gagcgtgggc ggcgtggtga actttgttac ccgtgccatt ccgcaggact ttggtatcga
     2581 ggcgggcgtg gaaggtcagc tcagcccaac ctcttcacaa aacaacccga aagagacgca
     2641 caacctgatg gtgggcggca cagcggacaa cggttttggc accgcgctgc tctactccgg
     2701 cacgcgcggc agtgactggc gcgagcacag cgccacccgc atcgacgacc tgatgctgaa
     2761 aagcaaatat gcgccggatg aggtgcacac cttcaacagc ctgctgcaat attacgacgg
     2821 tgaagccgac atgcccggtg gcctgtctcg cgcggattac gacgccgatc gctggcaatc
     2881 cacccgcccg tatgaccgct tctggggtcg tcgcaagctg gcgagcctgg gctaccagtt
     2941 ccagccagac agccagcata aattcaacat tcaggggttc tacacccaaa ccctgcgcag
     3001 cggctacctg gagcaaggca aacgcatcac cctctcgccg cgtaactact gggtgcgcgg
     3061 tattgagcca cgctacagcc agatctttat gatcggccct tccgcgcacg aagtgggcgt
     3121 gggctatcgc tatttgaatg aatcaacgca tgaaatgcgt tactacaccg ccaccagcag
     3181 cgggcagttg ccgtccggct caagccctta cgaccgcgat acgcgttccg gcaccgaggc
     3241 gcacgcctgg tatctggatg acaaaatcga catcggcaac tggaccatca cgccgggtat
     3301 gcgtttcgaa catatcgagt cataccagaa caacgccatc acaggcacgc acgaagaagt
     3361 gagctataac gcaccgcttc cggcgttgaa cgtgctctat cacctgactg acagctggaa
     3421 tctttatgca aacactgaag gctcgttcgg caccgtacag tacagccaga ttggcaaggc
     3481 tgtgcaaagc ggcaatgtgg aaccggaaaa agcgcgaacc tgggaactcg gtacccgcta
     3541 cgacgacggc gcgctgacgg cggaaatagg gctgttcctg attaacttta acaatcagta
     3601 cgactccaac cagaccaacg acaccgtcac tgcacgtggc aaaacgcgcc ataccgggct
     3661 ggaaacgcag gcacgttacg atctgggtac gctaacgcca acgcttgata acgtttccat
     3721 ctacgccagc tatgcgtatg tgaacgcgga aatccgcgag aaaggcgaca cctacggcaa
     3781 tctggtacca ttctccccga aacataaagg cacgctgggc gtggactaca agccaggaaa
     3841 ctggacgttc aatctgaaca gcgatttcca gtccagccag tttgcggata acgccaatac
     3901 ggtgaaagag agcgccgacg gcagtaccgg ccgcattccc ggcttcatgc tctggggcgc
     3961 acgcgtggcg tatgactttg gcccgcagat ggcagatctg aacctggcat tcggtgtgaa
     4021 aaacatcttc gaccaggact acttcatccg ctcttatgac gacaacaaca aaggcatcta
     4081 tgcaggccag ccgcgcacgc tgtatatgca ggggtcgttg aagttctgat gtgttaacgc
     4141 ccggcttgcc gggcttttag ctggaatgtg attatgttgg catttatccg ttttcttttt
     4201 gcaggcctgc tgctggtgat cagccacgcc tttgccgcca tggttcagga cgaacacggc
     4261 acgtttacac tcgaaaaaac gccacaacgg attgtggtgc tggaactctc gttcgccgat
     4321 gcgctggccg ccgtggacgt cagcccgatc ggtattgccg acgataacga tgcaaaacgc
     4381 atcctgcccg aagtgcgtgc gcacctgaaa ccgtggcagt ccgtcggaac gcgcgcgcag
     4441 ccgagcctgg aagccattgc cgctctgaaa ccagacctga tcattgccga cagcagtcgc
     4501 catgcggggg tttacatcgc cttgcagcaa atcgcgccgg tactgctgct taagtcccgc
     4561 aacgaaacct acgctgaaaa tttgcaatct gcggctatca tcggcgaaat ggtgggtaaa
     4621 aagcgagaga tgcaggcacg tctggaacaa cataaagaga ggatggcgca gtgggccagc
     4681 cagcttccca aagggacacg cgtggccttt ggcacatcac gcgaacagca attcaacctg
     4741 catactcagg agacctggac cggcagcgtg ctggcctctc tggggctgaa cgttcccgct
     4801 gcgatggtgg gtgcgtccat gccgtccatc ggcctggagc aactgctggc ggtcaatcct
     4861 gcctggctgc tggttgccca ctatcgcgaa gagagcattg ttaaacgctg gcaacaagat
     4921 ccgctctggc agatgttaac cgccgcgcag aagcagcagg ttgcttcggt cgacagtaac
     4981 acctgggcgc ggatgcgcgg tatttttgct gcagagcgta ttgccgctga cacggtaaaa
     5041 atcttccacc atcagccgct taccgttgtg aaatgaccgc gataaaacac ccggtgctgc
     5101 tgtgggggct tcccattgca gcacttatta ttattttctg gctgagtctg ttttgctact
     5161 cggccattcc tgtttccgga gcagatgcaa cccgcgccct gctgcctgga cacacgccaa
     5221 cgctaccaga agcgctggtg caaaaccttc gtttgccacg aagcctggtc gccgttctga
     5281 tcggcgcaag cctggcgctc gcgggcacgc tgctgcaaac cctgacccac aacccaatgg
     5341 cctctccttc actgctcggc attaacagcg gcgcggcgct ggctatggcg cttaccagcg
     5401 cgctaagtcc gacgccgatt gcaggctatt ctctgtcgtt catcgcggca tgcgggggcg
     5461 gcgtgagctg gctgctggtc atgaccgcag gaggcggatt tcgtcatacc catgacagaa
     5521 acaaactgat cctcgcgggt atcgcgctgt cggccttttg tatgggcctt acccgcatca
     5581 ccctgctgct ggccgaagat catgcttacg gcatctttta ctggctggca ggcggagtgt
     5641 cccacgcccg ctggcaggat gtctggcagc tcttgccggt ggtggtcact gcagtccctg
     5701 tcgtgttgct gctggcgaat caactgaacc tgctcaacct cagcgacagc accgcccata
     5761 cgctgggagt gaacctgacg aggctacgtt tggtcatcaa tatgttagtg ctgcttctgg
     5821 ttggtgcgtg cgtcagcgtg gcaggtccgg tggcgtttat cggtctgctg gtgccacatc
     5881 tggcgcgctt ctgggcaggc ttcgatcagc gcaacgtact gccagtgagc atgctgctgg
     5941 gggccacgct gatgctgctg gcagatgtac tcgcacgcgc gctggccttc cccggagatc
     6001 tgcccgcagg cgcagtgctg gcgctgattg gcagcccttg ctttgtctgg cttgtgagga
     6061 ggcgaggatg aaaattgcgc tggttatttt catcaccctt gccctggcgg gctgtgcgct
     6121 gttatcactc catatgggag tgatccccgt gccgtggcgc gcgctgctga ccgactggca
     6181 ggccggacgc gagcattatt atgtattgat ggagtaccga ctgccgcgct tgctgctggc
     6241 actgtttgtc ggtgcagccc tcgccgtggc gggcgtgctg atacagggga ttgtgcgcaa
     6301 ccctctggca tcaccggata ttctcggcgt taaccatgcc gccagcctgg cctctgtggg
     6361 ggctctactt cttatgccgt cactgcccgt gatggtgctg ccgctgctgg cctttgcggg
     6421 cggcatggcg gggttgatat tactgaagat gctggcaaag acccaccagc cgatgaagct
     6481 ggcgctcacc ggcgtggcgc tttctgcatg ctgggccagc ctgacggatt atctgatgct
     6541 ctcgcgccca caggatgtga acaacgccct gctgtggctg accggcagct tatggggccg
     6601 tgactggagc tttgtgaaga ttgccatccc gctgatgatt ttatttctgc cgctgagcct
     6661 gagtttttgc cgcgatctcg acctccttgc actcggcgat gcgcgcgcca ccacgctcgg
     6721 tgtgtcggtg ccccataccc gattctgggc tttgttacta gctgtcgcca tgacatctac
     6781 cggcgtggcc gcctgcggcc cgattagctt tattggtctc gtggtgccgc atatgatgcg
     6841 tagcatcacc ggtggacgtc accgcagact gctgcctgtt tcagccctga caggtgcgtt
     6901 gctgttggtg gttgccgatc tgctggcgag aattattcat cccccactgg agctcccggt
     6961 tggcgtgctg accgccatta tcggtgcgcc gtggtttgtc tggttgcttg tgagaatgcg
     7021 ataaatgact ttacgaactg aaaatctgac ggtcagttac gggacagaca aggtacttaa
     7081 cgacgtttca ctctcactgc caacggggaa gatcaccgcc ctgatcggtc ctaacggttg
     7141 cgggaaatcg acgctgttaa actgtttttc gcggctttta atgccgcagt ctggcaccgt
     7201 atttctcggc gataatccca taaatatgct ctcatcgcgc cagttggccc gcaggctttc
     7261 gctgctgcct cagcaccatt taacgccaga ggggatcaca gtccaggagc tggtttcgta
     7321 tggtcgtaat ccctggctgt cactctgggg gcgtctctcc gctgaagaca atgcacgagt
     7381 taatgtcgcc atgaaccaga cccggatcaa tcatcttgcc gttcgtcggt taaccgagct
     7441 ttccggcggt cagcgccagc gcgcatttct ggcgatggtc ctggcccaga atacgcccgt
     7501 tgtattactt gatgagccaa ccacctatct tgatatcaat caccaggtgg acctgatgcg
     7561 gttgatgggc gaactccgga ctcaggggaa aacggtggtc gctgtgctgc acgaccttaa
     7621 tcaggctagc cggtactgcg atcaactggt ggtaatggca aacggacatg ttatggcgca
     7681 aggcacacca gaagaggtga tgaccccagg attgctgaga acagtattca gcgtggaagc
     7741 ggaaatacac cccgagccgg tatctggcag gccgatgtgc ctaatgaggt agattgcaca
     7801 ggccgtaaga accaaaccac gactgaatga aactggactg gcgccagcaa gcctgttcag
     7861 actggggctg aacttttccg gactctgaaa gattaccaat actcatcgtc catccgcttg
     7921 ctttaggctg acaggttcat aattaacgca aaccagagct gtacaggctt gggcgcggct
     7981 ttcaaaccaa tcgtgatcac ggcaatcaat tttgaactct gcttaacgga catttctgta
     8041 taacccttac ggcaacgaaa aacgcgaagt taaaatttta gaaacccaaa aacgtgacat
     8101 gactaagttt agatttcagg ggggggagat caaaaaattt cgctctgtgc cagagcggac
     8161 attcacggag ctggttcatt accaatgagg ttgggctttt gaggataaat caatgatcag
     8221 acgccaacgt aaatcaaaag caaccctgga acggaattgc taatccagtt tctgaccatc
     8281 gatttttcta aaaagtgtgc gtttgctact acttaggtag gtgcagcttt cttaatcacc
     8341 ggcagccacg ttatacaggc cagttgatgg atcgattgtt atcaatgata tcttcatgag
     8401 tcggtgtctc acccagctta ccgaagctgg cataaagtga aggcagacgg gcccgtcctt
     8461 ctcccttttt cgccagaggg aaagcgcgaa gcatggtggc agcctcccag tcacaacaat
     8521 aggatggtgt gcacggctgc tgacgccatg attcagcgat agagccggaa aatacggggt
     8581 caaagccggt atcattaacc agagtcatcg tgacctgtac tgcggctgga tgatctcctg
     8641 ctactgcaac tgctaggcga ccgcggctcc cttcaggtag tctgttgtgg tcaactaaaa
     8701 ctggcctccg cgttagagtt tttccagtat cggttttctg attcgtttgg tggtaaccca
     8761 ccattatatt cgtgcggtct tagtgcgctg taatatccaa cgatatagtc cgttattgcg
     8821 tgagctgcat cgctgaagct tacatagccc gtcgctggca cccattcgtt cttcagactc
     8881 ctgaagaagc gctccattgg gctgttatcc cagcagtttc cacgccgact catactctgc
     8941 ctgatccggt atcgccacag taactgccgg aactgcctgc tcgtataatg actgccttga
     9001 tcgcctggaa catcaccccg acgggcttac cacgggtttc ccatgccatt tccagtgctt
     9061 tcatggt
//
LOCUS       DAJVUJ010000164         9047 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.164, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000164 DAJVUJ010000000
VERSION     DAJVUJ010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9047
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.164"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            197..1030
                     /gene="ppsR"
                     /locus_tag="OQQ48_003532"
     CDS             197..1030
                     /gene="ppsR"
                     /locus_tag="OQQ48_003532"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HCS1954164.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRTANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            1186..2232
                     /gene="aroH"
                     /locus_tag="OQQ48_003533"
     CDS             1186..2232
                     /gene="aroH"
                     /locus_tag="OQQ48_003533"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HCS1954165.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            2364..2555
                     /gene="hemP"
                     /locus_tag="OQQ48_003534"
     CDS             2364..2555
                     /gene="hemP"
                     /locus_tag="OQQ48_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HCS1954166.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(2559..3995)
                     /locus_tag="OQQ48_003535"
     CDS             complement(2559..3995)
                     /locus_tag="OQQ48_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HCS1954167.1"
                     /translation="MTLSFVTRWRDELPETYTALSPTPLNNARLIWHNIELANTLSIP
                     SSLFKNGAGVWGGEALLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TAEPGAMLMRVAPSHLRFGHFEHFYYRRESEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FSDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLIQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(4058..4771)
                     /gene="ydiV"
                     /locus_tag="OQQ48_003536"
     CDS             complement(4058..4771)
                     /gene="ydiV"
                     /locus_tag="OQQ48_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HCS1954168.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQVLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRGLLSLSQVYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTAEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            complement(5017..5481)
                     /locus_tag="OQQ48_003537"
     CDS             complement(5017..5481)
                     /locus_tag="OQQ48_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCS1954169.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKQQASIGTQIDKDKLLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            complement(5559..6308)
                     /gene="btuD"
                     /locus_tag="OQQ48_003538"
     CDS             complement(5559..6308)
                     /gene="btuD"
                     /locus_tag="OQQ48_003538"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HCS1954170.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVGLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLILTI"
     gene            complement(6308..6859)
                     /gene="btuE"
                     /locus_tag="OQQ48_003539"
     CDS             complement(6308..6859)
                     /gene="btuE"
                     /locus_tag="OQQ48_003539"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HCS1954171.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            complement(6922..7902)
                     /gene="btuC"
                     /locus_tag="OQQ48_003540"
     CDS             complement(6922..7902)
                     /gene="btuC"
                     /locus_tag="OQQ48_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HCS1954172.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISLGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELLIGVVTATLGAPVFIWLLLKARR"
     gene            complement(8111..8440)
                     /locus_tag="OQQ48_003541"
     CDS             complement(8111..8440)
                     /locus_tag="OQQ48_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1954173.1"
                     /translation="MNDDLERRVSCLESDVTEIKNNLITLTTRSESFATKSDVLEIRE
                     GLRLEMAESRQSLKSEMADLRQSLKVEMAEHRTELQKSFANQTWLLTGIVLSAMAVLV
                     AVVTVIK"
     gene            complement(8548..8910)
                     /locus_tag="OQQ48_003542"
     CDS             complement(8548..8910)
                     /locus_tag="OQQ48_003542"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1954174.1"
                     /translation="MKFHLDELKRMATLDNFARFLNESSTNEKCLSCGDTDMYMYLTN
                     IVEVGPEPKTAEECDLGTFVMLDYIGPFTGYPGYEGHDRENIHNYEFRLTCNRCGFVH
                     RYSARAFMNWVGKQGDEK"
     gene            complement(8913..>9047)
                     /locus_tag="OQQ48_003543"
     CDS             complement(8913..>9047)
                     /locus_tag="OQQ48_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCS1954175.1"
                     /translation="MNGGNILTLQKILGHAKIQTTMIYAHLAPDYLQDAVRFNPLGGA
                     "
BASE COUNT         2389 a         2396 c         2052 g         2210 t
ORIGIN      
        1 gcgttgcgca aacctgctct attcgacaat ttccacataa accatcggta taaacgaaac
       61 cataaaaatg aaatgctgtt ttcataaaaa ataaaattga aggttcattt tataaaccag
      121 acattacctc ctacgcttct gtgcgttttt aatttatgct ttcatagaat tatgtctgca
      181 tcacgggaag aacaaaatgg ataatgctgt tgatcgccac gttttttata tttctgatgg
      241 tacggcaata actgcggagg tattaggaca cgcagtaatg tcacaatttc ccgtcactat
      301 cagcagcatt acgctgccgt ttgtcgaaaa tgagagccgt gcacgggcag tgaaggatca
      361 gattgacgca atttatcacc agacaggtgt gcgcccgctg gtcttctact ccatcgtgtt
      421 gccggagatt cgcgccatca tcttgcaaag tgaaggcttt tgccaggata tcgttcaggc
      481 gctggttgcc ccgctacaac aagagatgaa actggatcca acgccgattg ctcatcgtac
      541 ccatggcctt aaccctaata atctcaataa atatgatgcg cgcattgcgg cgattgatta
      601 cacccttgcc cacgatgacg gcatttcgtt gcgcaatctg gatcaggctc aggtgatcct
      661 gctcggtgtt tctcgctgtg gtaaaacccc caccagtctg tatctggcaa tgcaatttgg
      721 tatccgcacg gcaaactacc cctttattgc cgacgatatg gataatctgg tgctacccgc
      781 gtcgctcaaa ccgcttcagc ataaattgtt cggcctgact atcgacccgg aacgtctggc
      841 ggcgattcgc gaggaacgtc gggagaacag tcgctatgcc tcgcttcgtc agtgcagaat
      901 ggaagtcgcg gaagtggaag ccttgtaccg taaaaatcag atcccgtgga ttaacagtac
      961 caattattcg gtagaagaga ttgccaccaa gatcctcgat atcatgggcc ttagtcgccg
     1021 aatgtactag agaactagtg cgctggtttg ttttttgtta tcatgctaac cacccggcga
     1081 ggtgtgacac acctcgcact tgaaatcagc tgcgattggt ttatcgtgat gcgcatcact
     1141 tcccggtagt cctgccgtag aagcaacaaa tttctgagac ttgtaatgaa cagaactgac
     1201 gaactccgta ctgcgcgtat tgagagcctg gtaacgcccg ccgaactcgc gctacggtat
     1261 cccgtaacgc ctggcgtcgc cacccatgtc accgactccc gccgcagaat tgaaaaaata
     1321 ctgaatggtg aagataagcg actgttggtc attattggcc cctgctcgat ccacgatctc
     1381 accgctgcaa tggagtacgc cacccgtctg cagtcgctgc gcaaccagta ccagtcacgg
     1441 ctggaaatcg taatgcgcac ctattttgaa aaaccacgaa ctgttgtcgg ctggaaaggg
     1501 ctaatctccg atccggattt aaacggcagc tatcgggtaa atcacggtct ggagctggcg
     1561 cgcaaattac ttttacaggt aaatgagctg ggtgtcccta ccgcgactga gtttctcgat
     1621 atggtgaccg gtcagtttat tgctgattta atcagttggg gcgcgattgg cgctcgtact
     1681 accgaaagtc agatccaccg cgaaatggct tcagcactct cctgtccggt aggttttaaa
     1741 aatggtaccg atggcaacac gcggattgcc gtggatgcta tccgcgcagc ccgcgccagc
     1801 catatgttcc tctcgccaga caaaaatggt cagatgacca tctatcagac cagcggcaac
     1861 ccgtatggcc acattattat gcgtggcggc aaaaaaccga attatcatgc cgatgatatc
     1921 gccgcagcct gcgatacgct gcacgagttt gatttacctg aacatctggt ggtggatttc
     1981 agccacggta actgccagaa gcagcaccgt cgccagttag aagtttgtga ggatatttgt
     2041 cagcaaatcc gcaatggctc tacggcgatt gctggaatta tggcggaaag tttcctgcgc
     2101 gaaggaacgc aaaaaatcgt cggcggtcag ccgctcactt acggtcaatc cattaccgac
     2161 ccgtgtctgg gctgggaaga taccgaacgc ctggtcgaaa aactcgcctc tgcggtagat
     2221 acccgcttct gaatgcgtgc ccattcctga tgggatgggc atttctgcgc aacttgttgt
     2281 cttctcaaca cattactgct tgctctggcc tgccataata ttgataataa gaatcattgt
     2341 tatatcaatt attattaatt tttatgcgtt atacggatag cagaaaactc acgcctgaaa
     2401 cggatgccaa tcacaagacc gcttccccgc agcccatccg gcgaattacc agccagacac
     2461 tgttaggtcc ggatggcaaa ctgattatcg atcatgacgg gcaagaatat ctgctccgta
     2521 aaactcaggc tggcaagctg ctgttaacta agtagccttt aactcgagca gctgacttcc
     2581 agccgtttac cccagtcagg tggacgactg acatagtcat catctctgtc gctgaaagga
     2641 tttcgcaacg cctcatgcag gcggtgcaat tccatcatat cacccttttc tgccgcttca
     2701 atcgcccgtt gcgccaacca gttgcgcaat accagagctg ggttaacgct ttgtatcagt
     2761 tgctgacgct cactatcgct aacctcgtct tgttgcaaac gcccccgata acgggcaaac
     2821 cagtcatcaa atgccgcacg atcaataaac tcatcacgta gcggtgacgc cgcgctgtgc
     2881 tgctcggtca gactcagcat gcggaatgtg cgggtataat cgctgcgctc tcgcgccatc
     2941 agactgaata attcattcag tagcgcgtta tcctcttttt gctccgtcat gaagcccagt
     3001 ttctgccgca tccgttgtcc ataatgcgtc aacaaaacct gctgatagct gtccagggcc
     3061 tcattcaggg catctacggc aacaaatggt gacaatgtct gcgccagacg ctgtaaattc
     3121 cacaacgcga cagcaggttg attatcaaag ctataacgcc cttgatgatc cgagtgatta
     3181 caaataaaac cgggttcgta atcatcaaga aaaccaaacg gcccgtaatc aagcgtcagt
     3241 cccagcagcg acatgttgtc ggtattcatc accccatgtg caaagccgac cgtctgccat
     3301 tgggcaatta acgacgcggt acgtgcgaca acatcgctaa accagagacg gtatttgtcc
     3361 tcatcatctt caagatgtga ccagtaatga cgaatagcaa agtcagctaa ctgacgaacc
     3421 ttttccgact cgcggcggta gtaaaaatgt tcgaaatgac caaagcgcag atgacttggt
     3481 gccacacgca tcagcatcgc gcctggctcc gccgtttccc gatacactgg cgaatcgctg
     3541 gtgacgatac ttaacgcgcg ggtcgtcgga atgcccagat aatgcatcgc ctcactggcg
     3601 agactttctc gtatcgtcga acgtaaaacc gcccgtccat cacccattcg cgaataaggc
     3661 gtcaggccag cacctttcag atgccagtcc attgtagtgc catcagcaag cagttgttcg
     3721 ccgagtaaaa tgccgcgccc atcacccagt tggcccgccc agacgccgaa ctgatgacca
     3781 ctgtaaacct gcgccagtgg tgacatacca ggcagtaggg cttcgccgcc ccagacacct
     3841 gcgccatttt taaacagcga cgatggaata ctcagcgtgt tagccagttc gatattatgc
     3901 caaatcagcc gggcattatt taaaggcgta ggggaaagtg ctgtataggt ttctggcaat
     3961 tcatcgcgcc agcgggtaac aaaagacagg gtcatagatc ctcctgtttg atagtgtaga
     4021 cggttactct cgtcttaaac accagcaaac agaagggtta tcgctgaacc aacgtcgtta
     4081 tctgatttat gggaacggca ggccacaggc atccctggag agcatggaaa tcgaaggggg
     4141 taatctgtgc caatatttct gccgtatcaa tgcctcccgc aatgatgcaa ttgcaacaag
     4201 gggaaatttg cgcctggatc gcacgaataa acggttcgaa ggagcggtgc gtgatttgtt
     4261 gctgaataaa acttttatcc agcatgacgc gggtgaaaag accatcaaaa accgctttca
     4321 ttgtgctatt gcctgctccc aaattaccta acacaagggg atacacttgc gataaggata
     4381 aaagaccgcg attatctttt ccttcattga gatgcgggta attctcatta atcaacagtt
     4441 caataaacgg atattttaat agttcgcctg cgtaattatc cctctctaat aataatgtag
     4501 ccacttgtgg cgttaaatta agccaggcaa agagtttatg ttgaataaaa aaatgttggc
     4561 atgattttag taattccaac tgctctgaaa ataattgcca atgttgctcc tcggtaagtt
     4621 gcgctataac ccgactcgtc gggatacgca ccgtgccatc ttcactggag aaatgggtaa
     4681 ttaactcaac gccgacaagg acttgctggt tatctcttat cggcaggaaa tagcaatcag
     4741 aatgataaag attctccaaa aaaatcttca tggtaaccgt ccctctcgaa ggatgttttt
     4801 cagtatccgg ctaaaaggga tgaggccata agacataaca attacagaag gagtaacttt
     4861 catttgttcc atgttaacca ctttttcagg ggtctttttt tagattatcc tgattataaa
     4921 cgaataatcc atttacggaa tttttgtctg caaaatacta ctgtattcag gagtaaatgg
     4981 acggcgatgt atcatcgccg tccatttttc gacatactag attcgtctcg cctgccagaa
     5041 atttttctgc caatagacat tatcaagtga ggaacgcatc actcccttgc tggtagaggc
     5101 gtggataaat tggttgttgg tatcgtaaat acctacatgc aaaccatttt gtccggaccc
     5161 cgttttgaaa aagaccaggt cacctggcag caacttgtct ttatcaattt gcgtgccaat
     5221 agaggcttgt tgtttggttt cacggggcag ctgcaaatcg aaacgatcgc gcatcgtcac
     5281 aaccacaaat cccgaacagt ccacaccgcg ccgcgtcatg ccaccataac gatacggcgt
     5341 gccatgccag ctttgtagct ggtcgttcaa accggcaata acggtaatcg aatcagaaag
     5401 tctggcattt ggcggcggtg ctttatggtg gctacacccg gccagaaaca gtgctgtgat
     5461 caaaataagg cagaaacgca ttccgtacgg ttcctctgtt ttttattctt gcattaattt
     5521 agcgtcgtaa ttacccgatt ttcaagatac taatgaaatc agatggtcaa aatcagcatt
     5581 ctgtgacctt cgatatccag acggcgaaaa ttcatcccat aggcctgcgc cagatttggc
     5641 ggcgtgagca cctcttctct gcgtccactg gccagcattt ttccaccttt tagcaaccac
     5701 gcccgatgcg catgacgcaa cgtgtggttg agatcgtgac tgctcatcac aatcgccagt
     5761 ccttgctgac acagcgcgct cagaattttg tctaacgcac tttgttgcgc aacatcaaga
     5821 ctgttcatcg gctcatcaag aagcagcaat tggcctgcgg gattggcttg tggtgtgatt
     5881 tgcaacacca ccgcagcaag acctacgcgt tgccattcac cgccggaaag ttgattggtg
     5941 ctacgaccga gtttgtcatc aagagccagc gcccctgcga catcattcag tagttcggta
     6001 cgcgttttat cgtgctgatg cagtgtcagg tagtgccaga ccggcattgc aaacggcggc
     6061 gtctgctgtt gtgaaagata ggcgcgatgc agcgcgagtt ttgttgcgga ccatgcttcc
     6121 aggggttgcc ccgcgaactg aatgcttccc ttaccgctgg tcattccggc cattcgtgca
     6181 agcaaggtac tcttacccgc gccattcggc cccaccaggt gcaggatctc cccagcccga
     6241 acctcgccag aaagtggccc taggcgggta gattccgcaa catcctgtaa ctgcatcaca
     6301 atagacatta ttttgccaac gccagtttaa tgctttccat cacaatgggg tcttccggcg
     6361 tcatatccgg ggaaaaacgc tggatgacca atccgtccct gccaactaaa aatttctcaa
     6421 aattccataa aatatcatca gggtacagcg gtgcacgacc tttgctgacc atacgggcat
     6481 agaatccgct ctcttccggc gcgactgcgg tcggcgctgc ggcaatcaat ttttgataca
     6541 gcggatggcg tccttcgccg ttaacttcaa tcttactgaa catcgggaac gtcacccccc
     6601 atgtggtggt acagtaagtt ttaatctctt catcgctgcc cggttcttgt tccagaaact
     6661 ggttgcacgg gaatcccagc accacaaaac ctcgatcggc ccaggctttc tgaatattct
     6721 ccaactgctc atattgcggc gttaagccac actttgaggc gacattgaca atcaacagca
     6781 cattaccggc gtacttctcc agcgtggtca cttcaccgtc gatatctttc actacggtcg
     6841 tcagaatgga atcttgcatc gtttctcctg ggtgtggtca gtaaaaatct tagcttttaa
     6901 tcatagaccg tctttttgcg gctaacgtct tgcttttaac aataaccaga taaacaccgg
     6961 cgcacccaac gttgcggtga ccacgccaat aagcagctct gcggcagcta atgccaggcg
     7021 cgctacaata tcggccagca gcaatgcgct cgcccctgcc agcgcgcagc cgggaagtaa
     7081 tacgcgatga tcggttaaac cacacaaccg aagaatatgt gggatcacca gaccaataaa
     7141 gccgatagca cccgccagcg ccacactgac gccaaccatc cagccggtcg cggccaccag
     7201 cacattgcgc cagaaccaca ggggtaaacc cagttgccgc gccgagatct cgccaagtgc
     7261 taacatattc atcggcctgg actgacaaca gatccacaac aacacgggga tcaatgccag
     7321 catcagccag ctttgccgcc agtctacgcc gccaaaaccg cccatcatcc agtacatcag
     7381 ctgacgtaaa tcaacagagg tggaaaagta gatagcccac gtcattagtg cgctacagat
     7441 aatccctaat gcaacgccag ccagcaataa ccgactggtc gaaagatgac gacgggcgaa
     7501 acgtaagagt attaaagtaa tgataagcgc gccagcaatc gcacacagcc ctagcgccca
     7561 gttggggagt tgcccttgcc caagcaatac cgcggcgata agccccacgc ctgcgccgtt
     7621 agagacgcca agtagtccag gttctgccag aggattttca aacaacgcct gcattacagc
     7681 gccggatata gccagcgccg caccaaccaa caatacagcc agcgtacgtg gcaggcgaat
     7741 ttgccagacg aacagttcgc cacgaggagt aaaccagtca cctagcgaga tccattgttc
     7801 accggcgcaa aggcttaaaa gaagcgccag cagcatcaaa actgacaggc ataataacca
     7861 gcgaatattt tgtcgctgtt gttggcgggc aagtgtcagc atggtatccg ttctgctgaa
     7921 gtgtcatggc gttgatttta cggtgactct tcgacagtga aaagaaaaaa ggccgcagag
     7981 cggccataaa cacaagtaaa aatcaacaag ttagataatt atcaaagact tacagacaca
     8041 caaaaacaca gccaaccaca acaaataaca gggatgtggt cactttgtgg atcatatcgc
     8101 cgccataaat ttacttaata acagtaacca cagccacaag cacggccatc gcagacagga
     8161 caatacctgt gagtaaccaa gtctgatttg caaatgattt ctgaagttca gtacgatgct
     8221 cagccatctc aactttcaga gactggcgta aatcagccat ctcagacttc agggattggc
     8281 gcgattctgc catctcaagc ctcaaccctt cgcgaatctc cagtacatca gatttggtag
     8341 caaaggattc gcttcttgtg gtgagagtaa tcaggttgtt tttgatttca gtcacatcac
     8401 tttccaggca actgacccgc cgttcaaggt cgtcattcat gccatcacct ccatcattac
     8461 cacttcctgt tacttggttg ttggatacat tataggcatt ctctttcttt tttctgaacc
     8521 gcgaaatatg tgcaacattc tctgccatca cttctcatca ccctgcttgc ctacccagtt
     8581 cataaaggcc cgggccgagt aacgatgaac aaacccacat ctgttacagg taagtcggaa
     8641 ttcatagtta tggatatttt cccgatcatg tccctcatat cccggatacc cagtaaatgg
     8701 accgatgtaa tcaagcataa caaaagtgcc taaatcacac tcctcagcag ttttcggctc
     8761 aggccccacc tcaacaatat tcgtcaaata catgtacata tccgtatcgc cacacgacaa
     8821 acatttttca ttcgtagacg actcattgag aaaacgcgca aaattatcga gcgttgccat
     8881 tcttttaagt tcatctaaat gaaacttcac tcttaagcac ctcctagtgg attaaacctc
     8941 accgcatcct gcaggtaatc cggcgcaaga tgggcataaa tcatcgttgt ctgaatcttt
     9001 gcgtgcccca gaattttctg gagcgtcaga atattgccgc cgttcat
//
LOCUS       DAJVUJ010000165         8812 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.165, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000165 DAJVUJ010000000
VERSION     DAJVUJ010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8812
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.165"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..971
                     /locus_tag="OQQ48_003544"
     CDS             <1..971
                     /locus_tag="OQQ48_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCS1954176.1"
                     /translation="TLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEWCSFLK
                     EHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVINNVNV
                     HYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAYGKFMS
                     TIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIYECDHF
                     VYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRITKVNNE
                     TVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(937..1059)
                     /locus_tag="OQQ48_003545"
     CDS             complement(937..1059)
                     /locus_tag="OQQ48_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1954177.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            1274..2959
                     /locus_tag="OQQ48_003546"
     CDS             1274..2959
                     /locus_tag="OQQ48_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HCS1954178.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELRDNFHTIKENWHRLMNRQR
                     LLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVANASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDIFSPADSWYVSQTPLIKTGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISAVL"
     gene            2997..5369
                     /locus_tag="OQQ48_003547"
     CDS             2997..5369
                     /locus_tag="OQQ48_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCS1954179.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYFSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVVGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYSGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQNLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            5426..8209
                     /locus_tag="OQQ48_003548"
     CDS             5426..8209
                     /locus_tag="OQQ48_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCS1954180.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAQPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKKALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFIEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIVQFTA
                     ADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            8571..>8812
                     /locus_tag="OQQ48_003549"
     CDS             8571..>8812
                     /locus_tag="OQQ48_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCS1954181.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
BASE COUNT         2588 a         1916 c         1917 g         2391 t
ORIGIN      
        1 ccactctggc tggcctggat tttttccgta aagttattca ctatcaacaa cgctatgcag
       61 gccaaaaacg tatttttaat gcattacaaa cgaatggcat tttattgaat aatgaatggt
      121 gttcctttct caaagaacat gaatttctgg ttggtatctc gatcgatggc ccccaggagt
      181 tacatgaccg ttacagacgc agtaattcag gtaacggtac ttttgcaaaa gtgatagcag
      241 ccatcgagcg tctgaaatca tatcaagttg agtttaatac gttaaccgtc attaataacg
      301 ttaatgtcca ttaccctctt gaggtttatc attttttaaa atctatcggc agtaaacata
      361 tgcaatttat cgaattgctc gaaaccggga cgccgaatat tgatttcagt ggtcatagtg
      421 agaacacatt ccgtatcatt gatttttctg tgcctcccac ggcttatggc aagtttatgt
      481 caaccatttt tatgcaatgg gttaaaaacg atgtgggtga aattttcatt cgtcagtttg
      541 aaagctttgt cagccgtttt ttggggaatg ggcatacaag ttgtattttc caggagtcct
      601 gcaaggataa tctggttgtt gaaagtaatg gagacattta cgaatgcgac cattttgtct
      661 atccacagta caaaattgga aacattaata aatctgaact caaaacgatg aacagtgtac
      721 aactgacagc gcaaaaaaaa cggatttcag cgaaatgtca gcaatgtgca tataaaccta
      781 tctgcaatgg cggttgtcct aagcatcgta ttactaaagt aaacaatgag actgtttctt
      841 atttttgcga aggttataaa atcctttttt caaccatggt accttatatg aacgccatgg
      901 ttgagttagc taagaacaga gtaccgcttt accacattat ggatgttgca aaacaaatgg
      961 agaataatta ataacgctct cttttaactc cattcttagc ctccttctta tgaccttaac
     1021 aaggcacccg acataaattc ctgtcgggtg tttcgtcatc atcatcttcc cttgcgaaaa
     1081 tcctggctta cgtggtatgc gtcagggcat tagcaatacc cttgattatc aatagcataa
     1141 ccataaccat aaccataacc actgaaaatt aaaaatttct atatatttca tattgttaaa
     1201 tgatacttaa ataaatttca tcagtaaatc attgccatag ataatcattg gatggcacaa
     1261 tcaattttcg ctaatgataa ccattcccat tacgttttgt atgttgatag caaagtatct
     1321 ctgtctgctg aagccctttt ggctgcgtaa gaataataaa acttcagtgt tgttaatcat
     1381 tattatcctc gcaatgatcc ttggcgtggt taaaattcag gtctggttaa acgactggaa
     1441 taatgatttt ttcaatgcgc taagccagaa agaaaccgat aagctttggc agcttgttct
     1501 ttggttccct gccctgctgg ggatctttgt attaatatcc gtaaacaaaa catggttaat
     1561 taaactactg actattcgct ggcgcgaatg gttaacagat tactacctta atcgctggtt
     1621 tgcagataaa aactactact tcacgcagat ctacggcgag cataaaaata cagataaccc
     1681 tgaccaacgt atcgctgaag acattctctt actgatcagc aaaacgctca gtctttcatt
     1741 tggcttcatc cagtcactta gcatgctgat cacctttacc gttattcttt ggcaaagcgc
     1801 gggtacgctc tcattcactg ttggtggaac agaatggaat atccaggggt atatggtcta
     1861 taccgtcgtg ctcattgtga tcggtggaac tttatttacc cataaggttg gtaagcgtat
     1921 tcgtccgctt aatgtggaaa aacaacgtag cgaagcgaca tttcgaacta atcttgtgca
     1981 gcataataag caggcagaac tgattgcgct aagcaatgct gagtctctac aacgccagga
     2041 gttgcgcgac aattttcata ccattaaaga gaactggcat cgtttaatga atcgtcagcg
     2101 gttgcttgat tactggcaaa atatttattc gcgctcgctt agcgttcttc cctactttct
     2161 gttattgccg cagtttatta gcgggcagat caatctgggc ggactgatga aatcgcgcca
     2221 ggcatttatg ctagtatcga acaatttaag ctggtttatt tataaatatg acgaacttgc
     2281 tgaactggct gcggttatcg atcgcttgta tgagttccat caactcactg aacagcgccc
     2341 tacgaataag cctaaaaatt gccaacatgc ggtacaagtg gctaatgcga gtattcgtac
     2401 gcctgataat aagatcatat tagagaactt gaactttcat gtttcgccag gcaaatggct
     2461 attactgaaa ggctactctg gtgcgggaaa aaccacactg cttaaaacat tatcccactg
     2521 ctggccgtgg tttaaaggtg atattttttc tcctgctgac agttggtatg tgtcacaaac
     2581 accgttaatc aaaaccggct tactgaaaga gattatttgt aaagcacttc ccctgcccgt
     2641 agacgataaa tcgttgagcg aagtactgca tcaggttggt cttgggaaat tggctgcgcg
     2701 tattcatgac cacgatcgct ggggagatat tctttccagc ggcgaaaaac aacgtatcgc
     2761 cctggcacga ttaattttac gacgtccgaa atggatattt cttgacgaaa ctacctctca
     2821 tcttgaggaa caagaggcta tccgcttact gcgtttagtg cgtgaaaaac tacccacaag
     2881 cggcgtcatt atggttacac atcaacccgg tgtctggaac ctggccgatg atatttgtga
     2941 cattagcgcg gttttataaa aacaataaaa gccctgctgt acggagatat aaataaatga
     3001 agcgagttct tattcctggc gtcattttat gtggcgctga tgtggcgcag gccgtcgatg
     3061 acaaaaacat gtacatgcat ttttttgaag agatgacggt ctatgctcct gtccctgtac
     3121 ccgtaaacgg caacacgcat tacaccagtg aaagcatcga gcgtttaccg accgggaatg
     3181 gcaatatcag cgatctgctg agaaccaacc ctgcggtacg catggattca acgcaaagta
     3241 cctcgttgaa ccagggagat attcgccctg agaaaatctc tattcacggt gcgtcgccct
     3301 accagaatgc ctatttgatt gacggtatta gtgcaactaa taacctgaac ccagcgaatg
     3361 agtccgatgc cagtagtgca accaatatta gcgggatgtc acaggggtat tatcttgatg
     3421 tcagcttact ggacaatgtg acgctttatg acagttttgt gccggttgaa tttggtcgct
     3481 tcaatggcgg ggtaattgat gcaaagatca aacgcttcaa cgctgatgat agcaaggtga
     3541 aattgggtta tcgcactacg cgttcggact ggttaacatc gcatatcgat gagaataaca
     3601 agagcgcatt taatcaaggt tcttcaggaa gtacttattt ctctccagat tttaaaaaga
     3661 acttttatac cttgtcgttt aatcaggagc tggctgataa ctttggcgtt accgccggtt
     3721 tatcgcgccg ccagtctgat atcacccgcg cggattatgt ttcgaatgac ggcattgtcg
     3781 tcggtcgggc acagtataaa aacgttatcg atactgcatt gagcaaattt acctggtttg
     3841 ccagcgaccg ctttacccac gatttaacct taaaatatac cggctccagc cgtgattata
     3901 ataccagcac cttcccgcag tctgatcgcg aaatgggtaa taaatcctat ggtcttgcat
     3961 gggatatgga tacgcaactc gcatgggcca aactacgtac caccgttggt tgggatcata
     4021 ttagtgatta tacccgtcac gatcatgaca tctggtacac cgaactttca tgtacatatg
     4081 gtgatattac agggcgttgt acccgtggcg gattaggaca catttcccag gctgtagata
     4141 attacacctt caaaacacgc ctggactggc aaaaattcgc cgtgggtgat gtttcgcatc
     4201 aaccctactc cggcgcggaa tacatctatt ccgatgcatg gactgaacgc cataaccagt
     4261 ctgaatccta tgtgattaat gctgcaggaa agaaaactaa ccataccatt taccataaag
     4321 gtaaaggcag cctaggaatt gacaactaca cgctgtatat ggcggatcac attagctggc
     4381 ggaatgtgtc gttaatgccc ggtgtgcgtt atgactatga caactatctg tcaaaccaca
     4441 atatctcccc gcgctttatg acggaatggg atatttttgc tgatcaaacc tcaatgatta
     4501 ccgccggtta taaccgttac tatggcggga atattcttga tatgggatta cgtgatatcc
     4561 gcaatagctg gacggaatcg gtatcaggta ataaaaccct gacgcgttat cagaatttga
     4621 aaacgcctta taacgatgaa ctggcaatgg gattgcagca aaaaatcggt aagaacgtta
     4681 ttgcacgcgc aaactatgtt taccgtgaag cgcatgatca aatcagcaaa agcagtcgta
     4741 ccgacagcgc gactaaaacc accattactg aatataacaa cgatggcaaa accaaaacgc
     4801 attcgtttaa cctcagtttt gaactggccg aacccctgca tatccgccag gtagatatta
     4861 acccgcaaat tgtctttagc tatatcaaga gcaagggcaa cttgtcgtta aacaatggtt
     4921 atgaggagag caataccggt gataaccagg tggtttataa cggtaatctg gtctcttacg
     4981 atagcgttcc agtggcagat tttaataacc cattaaagat ctccttaaac atggatttca
     5041 cgcatcaacc gagcgggtta gtgtgggcga atacgctggc gtggcaagaa gcgcgtaaag
     5101 ctcgcattat ccttggtaag acaaatgcgc aatacatcag cgaatattca gattacaagc
     5161 agtatgttga cgaaaaactg gatagcagcc tgacctggga cacccgcttg tcctggacgc
     5221 cacaatttct gaaacaacaa aacctgacga tcagtgccga tattctcaat gtactggata
     5281 gcaaaaccgc tgttgataca acgaataccg gtgtggcgac ctacgccagt ggccgtactt
     5341 tctggcttga tgtcagcatg aaattttaat agttcggatt ttacttaacc gggtaaattc
     5401 gcccggtttt cgcatggaga taattatgag aaacctctgt ttcttactga cgttagtggc
     5461 aactctgttg ctccccgggc ggctgattgc cgccgcctta ccgcaggatg aaaagttaat
     5521 taccgggcaa ctggacaatg gcttgcgata tatgatttat ccgcatgctc aaccaaagga
     5581 tcaggtaaat ttatggctgc aaattcatac cggttcattg caggaagaag acaatgagcg
     5641 cggcgtggct cattttgtag aacatatgat gtttaacggc acaaaaacat ggccgggtaa
     5701 taaagtcatc gaaacatttg agtcaatggg cctgcgtttt ggtcgcgatg ttaatgccta
     5761 taccagctat gacgaaacgg tgtatcaggt gagtttgccg actacgcaga aacaaaatct
     5821 gcaacaagtg atggcaatct tcagtgaatg gagtaatgcc gcaacctttg aaaaactcga
     5881 agtagacgct gaacgtggcg taattactga ggaatggcgt gcccatcagg atgcgaaatg
     5941 gcgcacctct caggcgcgcc gccctttcct gctggctaat acccgtaatt tagaccgtga
     6001 acctatcggc ctgatggata ctgtcgccac ggtcacaccg gcacaattgc gccaatttta
     6061 tcaacgctgg tatcaaccaa ataatatgac ctttatcgtg gtcggcgata tcgacagtaa
     6121 aaaagcgctg gcgctgataa aggataattt aagtaagctt ccggctaaca aagcagctga
     6181 aaatcgcgtc tggccgacaa aagccgaaaa ccacctgcgc tttaatatca ttaatgataa
     6241 agaaaaccgg gtgaacggca tcgcactcta ttatcgcctg ccaatggtac aagtgaacga
     6301 tgagcaaagc tttatcgaac aagctgaatg gagcatgtta gttcagctgt tcaatcaacg
     6361 tctgcaggaa cgcatacagt cgggcgagtt gaagactatt tctggcggca ctgcgcgcag
     6421 cgttaaaatt gcacccgatt atcagtcgct gtttttccgt gtaaatgcac gagacgataa
     6481 tatgcaggat gctgcgaatg cattaatggc agagttggca accattgatc agcatggttt
     6541 ttctgctgaa gaactcgatg atgtcaaatc tacccgcctc acctggctga aaaatgcggt
     6601 tgatcagcaa gctgaacgtg atttacgtat gctgaccagt cgcctggcat ccagctcatt
     6661 aaataatacg ccgttcttgt cgccggaaga gacatatcaa ctttcgaaac gtctgtggca
     6721 gcaaattacc gtgcaaagtc tggcggaaaa atggcagcag ttaagaaaga accaggacgc
     6781 attttgggag caaatggtaa acaatgagct tgccgccaaa aaagcattgt ctcctgcggc
     6841 tatcctggcg ctggaaaaag agtacgccaa caaaaagctg gcggcttaca tcttcccagg
     6901 cagaaattta tcgttaacag tagacgctga cccacaggcg gaaattagca gcaaagaaac
     6961 gctggctgag aatctgacat cattaacact ttccaatggt gccagggtta ttctggcaaa
     7021 atccgcgggt gaagagcaaa agctacaaat tactgccgta tcgaataaag gcgatttaag
     7081 tttccctgcg cagcaaaaat cacttatcgc gctggcaaat aaagcagtaa gcggaagcgg
     7141 cgttggtgag ctctcctctt ccagcctgaa acgctggagt gcggaaaatt cggtaaccat
     7201 gagcagtaaa gtcagtggca tgaatacgtt gctctccgtt agcgcgcgga ctaataaccc
     7261 tgaacctggt tttcagttga ttaaccagcg aatcacccac agcacgatta acgacaatat
     7321 ttgggcatcg ctacaaaatg ctcaaattca ggcgttgaaa acgctcgacc agcgtccagc
     7381 ggagaaattc gcccagcaga tgtatgagac gcgctatgct gatgaccgca cgaaattact
     7441 gcaagaaaat cagattgtac agtttactgc cgcagatgcg ctggctgccg atcgccagtt
     7501 gttttcatct ccagcggata tcacgtttgt cattgtcggt aatgtctcag aagacaaact
     7561 cgtggcgtta attacgcgtt acttaggatc aatcaaacac tctgattcgc cattagccgc
     7621 aggtaaacca ttaactcgcg cgacggacaa cgcatcggtt actgtaaaag aacaaaatga
     7681 acctgtggca caggtttcac agtggaagcg ttatgattcc cggacacctg ttaatctggc
     7741 gacgcgtatg gcgctcgatg cttttaacgt cgcactggca aaagatctac gtgttaatat
     7801 tcgtgaacag gcatctggag catacagcgt ttcttctcgc ctctcggttg atcctcaggc
     7861 caaagatatc agtcatttgc tggcttttac ttgtcaacca gaacgacatg atgaactgtt
     7921 aacgttagcg aatgaagtga tggttaagcg tctggctaaa gggatcagtg agcaagaact
     7981 gaatgaatac cagcaaaacg ttcagcgcag cctcgatatc caacagcgta gcgttcaaca
     8041 attagcgaac actattgtaa atagtcttat tcaatatgac gatcctgcag catggactga
     8101 gcaggagcaa ttgttgaaac aaatgacggt agagaatgtt aacactgccg ttaaacaata
     8161 tctttctcat ccggtaaata cttataccgg agtattattg ccaaaataat aacagccccg
     8221 tcaacacatc gttggcgggg attttagcaa tattcgctat ttttatgtaa taattttata
     8281 aatgcgttca aaataataat caagtactaa tagtgatatt ttaaggtctg atttttacgt
     8341 gataattcag gagacacaga atgcgcataa aaataacagc ataaaacacc ttaccaccac
     8401 ccaagaattt catattgtat tgtttttcaa tgaaaaaaca ttattcgcgt aatatctcac
     8461 gataaataac attaggattt tgttatttaa acacgagtcc tttgcacttg cttactttat
     8521 cgataaatcc tactttttta atgcgatcca atcattttaa ggagtttaaa atggataaga
     8581 agcaagtaac ggatttaagg tcggaactac tcgattcacg ttttggtgcg aagtctattt
     8641 ccactatcgc agaatcaaaa cgttttccgc tgcacgaaat gcgcgacgat gtcgcattcc
     8701 agattatcaa tgacgaatta tatcttgatg gcaacgctcg tcagaacctg gccactttct
     8761 gccagacctg ggacgacgaa aatgtccaca aattgatgga tttatccatt aa
//
LOCUS       DAJVUJ010000166         8735 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.166, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000166 DAJVUJ010000000
VERSION     DAJVUJ010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8735
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.166"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            245..1156
                     /gene="ttdA"
                     /locus_tag="OQQ48_003550"
     CDS             245..1156
                     /gene="ttdA"
                     /locus_tag="OQQ48_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HCS1954182.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            1153..1758
                     /gene="ttdB"
                     /locus_tag="OQQ48_003551"
     CDS             1153..1758
                     /gene="ttdB"
                     /locus_tag="OQQ48_003551"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HCS1954183.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRGKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            1807..3270
                     /gene="ttdT"
                     /locus_tag="OQQ48_003552"
     CDS             1807..3270
                     /gene="ttdT"
                     /locus_tag="OQQ48_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HCS1954184.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVFGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(3313..4326)
                     /gene="tsaD"
                     /locus_tag="OQQ48_003553"
     CDS             complement(3313..4326)
                     /gene="tsaD"
                     /locus_tag="OQQ48_003553"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCS1954185.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            4563..4778
                     /gene="rpsU"
                     /locus_tag="OQQ48_003554"
     CDS             4563..4778
                     /gene="rpsU"
                     /locus_tag="OQQ48_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCS1954186.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            4889..6634
                     /gene="dnaG"
                     /locus_tag="OQQ48_003555"
     CDS             4889..6634
                     /gene="dnaG"
                     /locus_tag="OQQ48_003555"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCS1954187.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            6829..8670
                     /gene="rpoD"
                     /locus_tag="OQQ48_003556"
     CDS             6829..8670
                     /gene="rpoD"
                     /locus_tag="OQQ48_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCS1954188.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         2110 a         2330 c         2334 g         1961 t
ORIGIN      
        1 acctgcaagt ctttggctaa aggccagcta ttcagcatcg ttaattatcc gcagttgtga
       61 taagcgcagt gtatttcgca aaacattgcc attccctgct ctggcgcaat attcattctt
      121 attttcgcgt tgattcacga actgttaatt acatattcac ggtggcaaaa aatataaaac
      181 cacattttta gtggtagttt atggcggtga atttttccag acaaatacaa aaactggagt
      241 tgccatgatg agcgaaagta ataagcaaca ggcagtgaat aagttgacag agattgtcgc
      301 taactttacc gccatgattt ctacccgaat gcctgatgac gtggtggata aactaaaaca
      361 gctaaaggat gccgaaacgt cgtcgatggg gaaaattatc taccatacga tgttcgacaa
      421 catgcaaaaa gcgattgacc tgaatcgtcc tgcctgtcag gacaccgggg agattatgtt
      481 cttcgttaaa gtcggttccc gcttcccact gcttggcgag ctgcaaagca tactcaaaca
      541 agccgtggaa gaggcaaccg tcaaagcgcc actacgtcac aatgcggtag aaatttttga
      601 cgaagtaaac accggcaaaa ataccggtag cggcgtaccg tgggtcacct gggacatcat
      661 ccccgacaat gacgatgcgg aaatcgaagt ttacatggca ggtggcggct gcacgctacc
      721 tggccgctcg aaagtgttaa tgccgtcaga aggctacgaa ggcgtggtga aattcgtctt
      781 cgaaaatatc tccaccctcg ccgtaaacgc ctgtccaccg gtactggtgg gcgtgggcat
      841 cgccacctcg gtggaaaccg ccgccgtact ctcgcgtaaa gccattttgc gcccgattgg
      901 cagccgccat cccaatccaa aagcggcaga actggagcta cgcctggaag aaggactcaa
      961 ccgtctgggg attggtccac aagggctgac tggcaacagt tcagtgatgg gcgtacatat
     1021 cgaatctgcc gcccgccatc cgtcaaccat cggcgttgct gtctctaccg gctgctgggc
     1081 gcatcgtcgc ggcacactgc tggttcatgc cgatctcacc tttgaaaatc tgtctcacac
     1141 ccggagcgcg ttatgaaaaa gatcctgaca accccgatca aagctgaaga tctgcaagat
     1201 attcgcgtcg gcgatgtgat ctacctgacc ggtacgctgg tgacctgccg tgacgtttgt
     1261 caccgccgct tgatcgaact gaaacgtccg atcccttacg atctcaacgg caaagcgatt
     1321 ttccacgctg gccccatcgt gcgcaaaaac ggcgataaat gggagatggt ttccgttggc
     1381 ccgacaacca gtatgcgtat ggaaagtttt gaacgtgaat ttatcgagca gaccggcgtg
     1441 aaactggtgg ttggcaaagg cggaatgggg ccgctgaccg aagaaggctg ccagaaattc
     1501 aaggcgctac atgtgatttt cccggcaggc tgcgcggtgc tggcggcaac ccaggtggaa
     1561 gagattgaag aagtgcactg gacagagctc ggaatgccgg agtcgctgtg ggtctgccgg
     1621 ggcaaagagt tcggcccgct gattgtctct attgataccc acggcaacaa tctgatagcc
     1681 gaaaacaaaa agctgttcgc cgaacgccgc gatcccatcg tggaagagat ctgcgagcac
     1741 gtccattaca tcaaataacc ctcccggaga ggctcgcccc ctctcctttt tcgcaggcat
     1801 aacacgatga aaccttccac tgaatggtgg cgataccttg cgccgctggc ggtcatcgcc
     1861 attattgctc taattccggt tcccgcaggg ctggagagtc atacctggct ctactttgcc
     1921 gtttttactg gcgtgatcgt tggactgatc ctcgaacccg tgccgggtgc cgtggtggcg
     1981 atggtgggta tctccatcat cgccatactc tctccctggc tgctgttcag ccccgaacag
     2041 ctcgcccagc caggctttaa gttcaccgcc aaatccctct cctgggcagt ttttggtttt
     2101 tctaattcgg ttatctggct cattttcgcc gcctttatgt ttggcacagg ctatgaaaaa
     2161 accgggcttg gacgccgcat tgcgctgatt ctggtgaaaa agatgggaca tcgcacgctg
     2221 tttctcggct atgcggtgat gttctccgag ctgatcctcg cacctgtaac accgtccaac
     2281 tcggcgcgtg gtgcggggat tatctatccc atcatccgta acctgccacc gctctatcaa
     2341 tcacaaccaa acgacagcag ttcgcgcagc attggctcgt acatcatgtg gatggggatt
     2401 gttgccgact gcgtgaccag cgccattttc ttgacggcga tggcaccaaa cttgctgtta
     2461 attggactga tgaaaagcgc atctcacgcc acgctgagtt ggggcgactg gttcctcggg
     2521 atgttgccgc tcagtatttt gctggtgctg ctggttccct ggctggctta tgtgctgtac
     2581 ccgccggtac tgaagtctgg cgatcaggtg ccgcgctggg cagagacgga actgcaggca
     2641 atgggcccgc tctgttcgcg tgaaaaacgg atgctggggc tgatggtagg cgcgctggtg
     2701 ctgtggattt tcggcggtga ttatatcgat gccgcgatgg tcggttacag cgtggtggca
     2761 ctgatgctgc ttctgcgcat tatcagttgg gacgacattg tcagtaataa agcggcgtgg
     2821 aacgttttct tctggctggc ctcgcttatc accctcgcta ccggactcaa caacactggt
     2881 tttattagct ggtttggcaa actgttagca ggcagcttaa gcggttattc gccaacgatg
     2941 gtgatggtgg cgttgattgt ggtgttttat ctgctgcgct actttttcgc cagcgccacg
     3001 gcgtatacct ccgccctcgc accgatgatg attgccgctg cgctggcgat gccggaaatc
     3061 ccgctgccgg tgttctgcct aatggttggc gcggcaattg gtctggggag cattcttaca
     3121 ccatacgcca ccggacccag tccgatttac tacggtagtg gttatctgcc aacggcggat
     3181 tactggcgac tgggggcgat ttttgggctg atattcctcg tgttgctggt gattaccggc
     3241 ttactgtgga tgcccgtggt gttgctttaa gtgaggacgg tgccggaaat tgctctccgg
     3301 cacccaatag ttttacgcag ccggtaactc cgccagcggc cagcgcggac gcacgctaac
     3361 gccgagatcc gccgtcgcgc ctgctttaaa ccgcaccatt ccggcatagg cgatcatcgc
     3421 gccgttatca gtacaaaact ccggacgcgc gtagaacact tcgccgcggc gttttttcat
     3481 catttccgcc agcttcgccc gtaacgtgcg gttagcactc acgccgcccg ccatgaccag
     3541 tcgcttaaag cccgtctgat ccaacgctcg cttacactta atcatcaacg tatcgaccac
     3601 cgcatcttca aaggcgcggg cgatatcagc acgcgtctgg tcgtcggtgc cgttgtcacg
     3661 aatcgtgttc gccgcaaagg ttttcagacc agaaaagctg aaatccagcc ccggacggtc
     3721 ggtcatcgga cgcgggaaaa caaagcgccc ggcagtaccc tgagccgcca ttttcgacag
     3781 taacggtccg ccaggataat ccagccccag cagcttcgcg gttttatcaa acgcttcacc
     3841 ggcggcatca tcgatagact cgccgagcag ctcgtactga ccaatgccag tcacgctgat
     3901 taactgcgta tgaccgccgg acaccagcag cgcgacaaac ggaaattccg gcgggttatc
     3961 ttccagcatc ggcgctaaca gatgcccttc catatggtgt accgggattg ccggaacgtt
     4021 ccaggcaaac gccagagaac gccccacggt tgcgccaacc agcagtgcgc cgactaatcc
     4081 agggcctgcg gtataggcca cagcatcaat gtcttttgcc gttaagccag actcctttag
     4141 cgccgcctgg atcaacggta cggttttgcg cacgtgatcg cgggaggcca gttcaggcac
     4201 gacgccgccg tagtcagcgt gcaatttcac ctgactatac aattggttgg ctaacaaacc
     4261 tttttcatcg tcgtaaatgg cgatgccggt ttcatcgcag gaagtttcaa tacccagtac
     4321 acgcatgact tgttttacct cgctttatta ccgcgcagtg taggaccaat gcgggttgat
     4381 gtaaaacttt gttcgcccct ggagaaagcc tcgtgtatac tcctcaccct tataaaagtc
     4441 cctttcaaaa aggccgcggt gctttacaaa gcagcagcaa ttgcagtaaa attccgcacc
     4501 attttgaaat aagctggcgt tgatgccagc ggcaaaccga attaatcaaa ggtgagaggc
     4561 acatgccggt aattaaagta cgtgaaaacg agccgttcga cgtagctctg cgtcgcttca
     4621 agcgttcctg cgaaaaagca ggtgttctgg cggaagttcg tcgtcgtgag ttctatgaaa
     4681 aaccgactac cgaacgtaag cgcgctaaag cttctgcagt gaaacgtcac gcgaagaaac
     4741 tggctcgcga aaacgcacgc cgcactcgtc tgtactaatt ccccgagagc gttgctctcc
     4801 gatcagaccg agttgtagtt gtaaggccgt gcttccgaaa ggaatgcgcg gcttattttc
     4861 gtttatgaat tgctaaaaat cggggcctat ggctggacga atcccacgcg tattcattaa
     4921 tgatctactg gcacgcactg acatcgtcga tctgatcgat gcccgtgtga agctgaaaaa
     4981 gcagggcaag aatttccacg cgtgttgtcc attccacaac gagaaaaccc cgtcattcac
     5041 cgttaacggt gagaaacagt tttaccactg ctttggatgt ggcgcgcacg gcaacgcgat
     5101 cgacttcctg atgaactacg acaagcttga gttcgtcgaa acggtcgaag agctggcagc
     5161 aatgcacaat cttgaagtgc catttgaagc aggcagcggc cccagccaga tcgagcgcca
     5221 tcagaggcaa acgctttatc agttgatgga cggtctgaat acgttttacc aacaatcttt
     5281 acaacaacct gttgccacgt ctgcgcgcca gtatctggaa aaacgcggat taagccacga
     5341 ggttatcgct cgctttgcga ttggttttgc gccccccggc tgggacaacg tcctgaagcg
     5401 gtttggcggc aatccagaaa atcgccagtc attgattgat gcggggatgt tggtcactaa
     5461 cgatcaggga cgcagttacg atcgtttccg cgagcgggtg atgttcccca ttcgcgataa
     5521 acgcggtcgg gtgattggtt ttggcgggcg cgtgctgggc aacgataccc ccaaatacct
     5581 gaactcgccg gaaacagaca ttttccataa aggccgccag ctttacggtc tttatgaagc
     5641 gcagcaggat aacgctgaac ccaatcgtct gcttgtggtc gaaggctata tggacgtggt
     5701 ggcgctggcg caatacggca ttaattacgc cgttgcgtcg ttaggtacgt caaccaccgc
     5761 cgatcacata caactgttgt tccgcgcgac caacaatgtc atttgctgtt atgacggcga
     5821 ccgtgcaggc cgtgatgccg catggcgagc gctggaaacg gcgctgcctt acatgacaga
     5881 cggccgtcag ctacgcttta tgtttttgcc ggatggcgaa gaccctgaca cgctggtacg
     5941 aaaagaaggt aaagaagcgt ttgaagcgcg gatggagcag gcgatgccac tctccgcatt
     6001 tctgtttaac agcctgatgc cgcaagttga tctgagtacc cctgacgggc gcgcacgttt
     6061 gagtacgctg gcactgccat tgatatcgca agtgccgggc gaaacgctgc gaatatatct
     6121 tcgtcaggaa ttaggcaaca aattaggcat acttgatgac agccagcttg aacgattaat
     6181 gccaaaagcg gcagagagcg gcgtttctcg ccctgttccg cagctaaaac gcacgaccat
     6241 gcgtatactt atagggttgc tggtgcaaaa tccagaatta gcgacgttgg tcccgccgct
     6301 tgagaatctg gatgaaaata agctccctgg acttggctta ttcagagaac tggtcaacac
     6361 ttgtctctcc cagccaggtc tgaccaccgg gcaactttta gagcactatc gtggtacaaa
     6421 taatgctgcc acccttgaaa aactgtcgat gtgggacgat atagcagata agaatattgc
     6481 tgagcaaacc ttcaccgact cactcaacca tatgtttgat tcgctgcttg aactgcgcca
     6541 ggaagagtta atcgctcgtg agcgcacgca tggtttaagc aacgaagaac gcctggagct
     6601 ctggacatta aaccaggagc tggcgaaaaa gtgatttaac ggcttaagtg ccgaagagcg
     6661 atcgggaagc ccccgacagc cgcactgaga ggcagcggca aatatataag tacgccctcg
     6721 taattatcgt tggcggtaaa caaccgttgg atttcagcgt taacggctga aggacatcgg
     6781 gtcaatcgcc caacaccaac ctcatgaaat aagtgtggat accgtcttat ggagcaaaac
     6841 ccgcagtcac agctgaaact tcttgtcacc cgtggtaagg agcaaggcta tctgacctat
     6901 gccgaggtca atgaccatct gccggaagat atcgtcgatt cagatcagat cgaagacatc
     6961 atccaaatga tcaacgacat gggcattcag gtgatggaag aagcaccgga tgccgatgat
     7021 ctgatgctgg ctgaaaacac cgcggacgaa gatgctgccg aagccgccgc gcaggtgctt
     7081 tccagcgtgg aatctgaaat cgggcgcacg actgacccgg tacgcatgta catgcgtgaa
     7141 atgggcaccg ttgaactgtt gacccgcgaa ggcgaaattg acatcgctaa gcgtattgaa
     7201 gacgggatca accaggttca atgctccgtt gctgaatatc cggaagcgat cacctatctg
     7261 ctggaacagt acgatcgtgt tgaagcagaa gaagcgcgtc tgtccgatct gatcaccggc
     7321 tttgttgacc cgaacgcaga agaagatctg gcacctaccg ccactcacgt cggttctgag
     7381 ctttcccagg aagatctgga cgatgacgaa gatgaagacg aagaagatgg cgatgacgac
     7441 agcgccgatg atgacaacag catcgacccg gaactggctc gcgaaaaatt tgcggaacta
     7501 cgcgctcagt acgttgtaac gcgtgacacc atcaaagcga aaggtcgcag tcacgctgcc
     7561 gctcaggaag agatcctgaa actgtctgaa gtattcaaac agttccgcct ggtgccgaag
     7621 cagtttgact acctggtcaa cagcatgcgc gtcatgatgg accgcgttcg tacgcaagaa
     7681 cgtctgatca tgaagctctg cgttgagcag tgcaaaatgc cgaagaaaaa cttcattacc
     7741 ctgtttaccg gcaacgaaac cagcgatacc tggttcaacg cggcaattgc gatgaacaag
     7801 ccgtggtcgg aaaaactgca cgatgtctct gaagaagtgc atcgcgccct gcagaaactg
     7861 cagcagattg aagaagaaac cggcctgacc atcgagcagg ttaaagatat caaccgtcgt
     7921 atgtccatcg gtgaagcgaa agcccgccgt gcgaagaaag agatggttga agcgaactta
     7981 cgtctggtta tttctatcgc caagaaatac accaaccgtg gcttgcagtt ccttgacctg
     8041 attcaggaag gcaacatcgg tctgatgaaa gcggttgata aattcgaata ccgccgtggt
     8101 tacaagttct ccacctacgc aacctggtgg atccgtcagg cgatcacccg ttctatcgcg
     8161 gatcaggcgc gcaccatccg tattccggtg catatgattg agactatcaa caaactcaac
     8221 cgtatttctc gccagatgct gcaagagatg ggccgtgaac cgacgccgga agaactggct
     8281 gaacgtatgc tgatgccgga agacaagatc cgcaaagtgc tgaagatcgc caaagagcca
     8341 atctccatgg aaacgccgat cggtgatgat gaagattcgc atctggggga tttcatcgag
     8401 gataccaccc tcgagctgcc gctggattct gcgaccaccg aaagcctgcg tgcggcaacg
     8461 cacgacgtgc tggctggcct gaccgcgcgt gaagcgaaag ttctgcgtat gcgtttcggt
     8521 atcgatatga acaccgacca cacgctggaa gaagtgggta aacagttcga cgttacccgc
     8581 gaacgtatcc gtcagatcga agcgaaggcg ctgcgcaaac tgcgtcaccc gagccgttct
     8641 gaagtgttgc gtagcttcct ggacgattaa tcggtaggcc ggatcaggcg ttacgccgca
     8701 cccggcacta gtccctctgc acaaacgcca ccttt
//
LOCUS       DAJVUJ010000167         8713 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.167, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000167 DAJVUJ010000000
VERSION     DAJVUJ010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8713
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.167"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            122..187
                     /gene="yobI"
                     /locus_tag="OQQ48_003557"
     CDS             122..187
                     /gene="yobI"
                     /locus_tag="OQQ48_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HCS1954189.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            326..937
                     /gene="ruvA"
                     /locus_tag="OQQ48_003558"
     CDS             326..937
                     /gene="ruvA"
                     /locus_tag="OQQ48_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCS1954190.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            946..1956
                     /gene="ruvB"
                     /locus_tag="OQQ48_003559"
     CDS             946..1956
                     /gene="ruvB"
                     /locus_tag="OQQ48_003559"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCS1954191.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(2006..2791)
                     /gene="znuB"
                     /locus_tag="OQQ48_003560"
     CDS             complement(2006..2791)
                     /gene="znuB"
                     /locus_tag="OQQ48_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HCS1954192.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLALDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLIAIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(2788..3543)
                     /gene="znuC"
                     /locus_tag="OQQ48_003561"
     CDS             complement(2788..3543)
                     /gene="znuC"
                     /locus_tag="OQQ48_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HCS1954193.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            3622..4554
                     /gene="znuA"
                     /locus_tag="OQQ48_003562"
     CDS             3622..4554
                     /gene="znuA"
                     /locus_tag="OQQ48_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HCS1954194.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLLGAKQV
                     TIAQLEDVKLLLMKSIHGDDDDHDHAEKSEEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            4570..5892
                     /gene="mepM"
                     /locus_tag="OQQ48_003563"
     CDS             4570..5892
                     /gene="mepM"
                     /locus_tag="OQQ48_003563"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HCS1954195.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            6012..6983
                     /gene="lpxM"
                     /locus_tag="OQQ48_003564"
                     /gene_synonym="msbB"
     CDS             6012..6983
                     /gene="lpxM"
                     /locus_tag="OQQ48_003564"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCS1954196.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMVGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLQIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRRPGEIQPYKRKDLYPIK"
     gene            complement(7072..>8713)
                     /locus_tag="OQQ48_003565"
     CDS             complement(7072..>8713)
                     /locus_tag="OQQ48_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HCS1954197.1"
                     /translation="IDGLPHVDFSRVDKFFTYADAFRESLPIISGELYFEAHQGCFTS
                     ESATKAHNRNMENKLHDAEFFTTITNNMTSILRSEFDEIWKEMLTLQFHDILPGSCIS
                     RVYLETEKEYIKLEAKTEKIISDTQSTLLSKIDTSSYKDPHILFNTTCFARNEWININ
                     NNWLKARVNSYGYAVIDPKNKIVNGLKAESRSIENNYIKLLFSENGDLISLYDKRYGK
                     EYITENMHSEIRAYHEDAGFFAAWDFASNYRDGESYVLLAEKMTTVISGPKTTMTLIY
                     HYNSSYLRFAFTLTQDSPRVDVQTFIDWHEPNVSLKVKFPVSVQTSLAQCQIQFGVID
                     RPTHSDDSFAFAKDEIPAHHWVDLSDRETGIALIAKNKYGYRVKDQTLELTLLRSQHK
                     PGEIVKTDNYDNFLINNFADIGKQKFIFSLFPHHGDYRVGGVINQAYIMNHPLQVVPV
                     KPHPGELPPSLSFFAIDTNSVIIETIKLAEDGDGIIVRLYESHGLEISAMLSWVCNYH
                     YVQYVDLQENKLDDSFYCNQYCNLEFKPFEIITLRLTQ"
BASE COUNT         2372 a         2007 c         2127 g         2207 t
ORIGIN      
        1 tgtaaagacg atatctccaa tttcatattc agcagggtaa ttaatattca cgttgtcatt
       61 ccattgaaat agatacgaat atacgtaata gcataaaaaa attatttttt caggaggtgc
      121 aatgtatatt tttattacgc atttcttcac tgaatacgta atatttaaat atttgcttcc
      181 aatataacct gtagaataaa tcatactgcg ccatttttca gttcatcgag acacctcgca
      241 agttttcttc atccttcgct ggatatctat ccagcatttt tttatcatac agcattatct
      301 ttgattcatt acgcaggagc gtcatgtgat aggcagactc agaggcatca tcattgaaaa
      361 acaacccccg ctggtgttaa ttgaagtggg cggcgtaggc tatgaagtgc atatgccgat
      421 gacctgtttt tatgaactcc ctgaagcggg tcaggaagcg atcgttttca cccactttgt
      481 ggtgcgtgaa gacgcgcaac tgctgtacgg ttttaacaat aaacaagagc gcacattgtt
      541 caaagagttg atcaaaacca acggcgtcgg cccgaagttg gcgctggcga tcctctccgg
      601 aatgtcagcg cagcagttcg ttaatgccgt tgaacgtgaa gaagtggggg cactggtgaa
      661 actgccgggt attggcaaaa aaaccgccga acgcttgatt gttgaaatga aagaccgatt
      721 taaaggtttg catggcgatc tgtttacccc tgccgccgac ctggtactca catcacctgc
      781 cagcccggcg accgacgatg ctgaacagga agcggttgcc gcgctggtgg cgctgggcta
      841 taaaccacaa gaagcaagcc gcatggtgag caaaatcgct cgccctgacg ccagcagtga
      901 aacattaatt cgcgaagccc tacgcgccgc gttatgaggt aaaggatgat tgaagcagac
      961 cgtctgattt ctgccggtac cactttgccg gaagatgtag cagatcgcgc cattcgccct
     1021 aaattactgg aagagtatgt tggtcagccg caggttcgtt cacagatgga gattttcatc
     1081 aaagcagcga aactgcgcgg cgatgccctc gatcatttgt tgatttttgg tcctccgggg
     1141 ttgggtaaaa ctacgcttgc caacattgtc gccaatgaaa tgggcgttaa tttacgcacg
     1201 acttctggtc cggtgctgga aaaggcgggc gatctggcag cgatgctcac taaccttgaa
     1261 ccgcatgacg tgctgtttat tgatgagatc caccgtttat cgccagttgt tgaagaagtg
     1321 ctgtacccgg caatggaaga ctaccaactg gatatcatga ttggtgaagg tccggcggca
     1381 cgctccatta aaattgattt gccgccgttt accctgattg gcgcaaccac gcgcgcaggt
     1441 tcgctgacat caccgttgcg cgaccgtttt ggtattgtgc aacgtctgga gttttatcag
     1501 gtgccggatc tgcaatatat cgttagtcgc agtgcacgct ttatggggct tgagatgagt
     1561 gatgacggcg cgctggaagt tgctcgtcgc gctcgcggta cgccacgtat tgccaaccgt
     1621 ctgctgcgtc gagtgcgtga tttcgccgaa gtgaagcacg atggcaccat ctcggcagat
     1681 atcgctgctc aggcgctgga catgttgaat gtcgatgctg aaggtttcga ttatatggac
     1741 cgcaaattgt tgctggcggt aatcgataag ttctttggtg ggccggtagg tctggataac
     1801 ctggcggcgg ctattggcga agaacgtgaa accattgagg atgtgctgga accttatttg
     1861 attcagcaag gctttttgca gcgtacaccg cgtgggcgta tggcgacgac gcgggcgtgg
     1921 aatcactttg gcataacgcc gccagaaatg ccgtaagtcg gattggtcgc tcgcgccgca
     1981 tccgacaaat gtgttcagcg atagattagc tggcttgctt tttcatcata ctgataataa
     2041 ataacagtgc tgcacatagc accaccgacg gacccgccgg cgtatcgtaa aatgcggaaa
     2101 aggttaaacc gcccgttacg gcaaccatcc ccaccaacac agcgacacct gccatctgtt
     2161 ccggcgtgcg ggcaaagcga cgtgcagtag cagcaggaat aatcagcagt gaagtaataa
     2221 tcaacgcgcc aacaaatttc atcgctacac caatcgtcaa tgccgtcacc agcatcaaca
     2281 acaatttcac gcgctgtaat ttcacaccat caacaaacgc cagatccggg ctaatcgtca
     2341 tcgacagcaa attgcgccat tgccagaaca aaatagccac cacgatgacc acgccaatcg
     2401 caatagcgat gagatcttct ggcgtcactg ccagcaaatc accgaacagg taagccatca
     2461 aatcaacacg aatgttagac atcagactaa cgaccaccag gcccagcgac agggcactgt
     2521 gcgccataat ccctaataac gtgtcaagcg ccagctgtgg acgtttctcc agccatacca
     2581 gaccgcccgc caggagcagc gtaacggcaa ttaccgcata gaatggattc acgtccagca
     2641 acaaaccaaa cgcgacgcca agtaatgagg catgagccag cgtatcaccg aaataagaca
     2701 tacgacgcca gactacaaac gaacccagcg gacccgcggc acaggcgagc atgatcccgg
     2761 ctaaccaacc gggaaataat aattcaatca tgagcgatca tttccccgac gcaaaacaat
     2821 tcgtccctgt aaatcgtgac gatgattatg atgatggcga tagataccca gttgttcagc
     2881 accacgagga ccaaacatag aaataaactc cggatgtagg gaaacaactt ccggtgtgcc
     2941 ggaacaacaa atgtggtgat tcagacacag cacttcatcg gtttttgcca ttaccagatg
     3001 cagatcgtga gagaccatta aaacgccaca atccagttcg cgacgcaact ggtcaataag
     3061 gtcatataac gccacctggc catttacatc cacgccttga gtgggttcat ccagcactaa
     3121 taattgcggt cgatttaaca atgctcgcgc taacagtaca cgctgtgttt cgccaccaga
     3181 gagcttttgc atcggtgcgt taatcagatg cccggcctgg acacgtttca gtgcaggcaa
     3241 aatatcttct ttatgtgtgc cagggcgtaa gcgtaaaaaa cggtttacgg tcagtggcaa
     3301 cgtggtgtcg agatacagct tctgcggtac atagccgatg cgtagttttc cgttgcgctt
     3361 gataacccct tcatcgggtg ttaccagccc gagcactacc cgtaccagtg tcgacttacc
     3421 tgcgccattt ggcccaagta aagtcaaaat ttttccaggt ttaagttcca gcgacacatc
     3481 agagaggacg cggcgttggc caaaagaaac tgagacattt tccagggaaa ccagacttgt
     3541 catgttaatt ttagtcttgc agtagtcatg agatgttata atatcacact tctcatattc
     3601 attacgatga ttggtcgcat tatgttacat aaaaaaacgc ttcttttcgc agcattatcc
     3661 gccgctctct ggggaggtgc aacacaggcc gcagatgccg ccgttgtcgc ttcgcttaaa
     3721 cccgttgggt tcatcgcttc tgccattgct gatggtgtaa cagaaacgga ggttttactg
     3781 cctgacggcg cttcagaaca tgattattca ctgcgtccat cggatgtaaa acgcttacag
     3841 aacgcggact tagtcgtttg ggttggcccg gagatggaag cgtttatgca aaaaccggta
     3901 agcaaattac taggagcgaa gcaggtaacg attgcgcagc ttgaagatgt gaaactgcta
     3961 ctgatgaaaa gtattcacgg cgatgatgat gatcacgacc acgcggaaaa aagtgaagaa
     4021 gatcaccatc acggcgattt caacatgcat ctttggcttt ccccagagat agcgcgggct
     4081 acagcggttg caatccatgg aaaattagtg gaacttatgc cgcaaagtcg agccaaactt
     4141 gacgccaacc tgaaggattt tgaggcacaa ttagcctcaa ccgaaacgca ggttggtaac
     4201 gagctcgcgc cgctcaaggg gaaaggttat ttcgtttttc acgatgctta cggctatttc
     4261 gaaaaacagt tcggactgac accgcttggt cattttaccg ttaaccctga gattcaacct
     4321 ggcgcgcagc gtttacatga aataagaaca cagttggttg agcaaaaagc aacctgcgtt
     4381 tttgctgagc cacagttcag gccagcggtc gttgagagcg tcgcacgagg gacatccgtt
     4441 cgtatgggaa cgttggatcc cttggggacg aatatcaaac tgggtaaaac aagctattca
     4501 gaattcctga gtcaattagc caaccagtat gcgagctgcc tgaaaggaga ttaatgagga
     4561 agtgattacg tgcaacagat agcccgctct gtcgccctgg cgtttaataa tttaccgcga
     4621 ccacaccgcg ttatgttggg gtcgctcacc gttcttactc tggccgtcgc tgtctggcgg
     4681 ccctatgttt atcaccgcga tgccacgcca attgtcaaaa ccattgagct ggaacagaac
     4741 gaaattcgtt cgctcttacc tgaagccagt gagccgattg atcaagctgc acaagaagat
     4801 gaagccattc cccaggacga actggatgac aaaatcgccg gtgaagcggg cgtgcatgaa
     4861 tatgttgttt ccactggcga tacgctaagc agcattctca atcagtatgg tattgatatg
     4921 ggtgatatca cccaactggc tgcggccgac aaagaattgc gtaacctgaa aatcggtcaa
     4981 caactctcct ggacattaac cgcggacggc gaactgcagc gcctcacctg ggaagtgtct
     5041 cgtcgtgaaa cccgaaccta tgaccgtact gccgctaacg gttttaaaat gaccagcgaa
     5101 atgcagcaag gagagtgggt taacaatctg ctgaaaggta ccgtcggagg gagctttgtt
     5161 gccagcgcca gaaacgccgg tttaaccagc gccgaagtga gcgcagtgat taaagccatg
     5221 cagtggcaaa tggatttccg caaactgaaa aaaggcgatg aatttgcggt gttaatgtct
     5281 cgagagatgc ttgatggtaa acgtgagcaa agccagctgc tgggcgtacg tttgcgttca
     5341 gaaggtaaag attattacgc aatccgcgct gaggatggca aattctacga ccgtaacggt
     5401 actggtctgg cgaaaggatt cttgcgattc ccgacggcga aacagttccg tatctcgtct
     5461 aactttaacc cgcgtcgtac taatccggtg accggtcgcg ttgcaccaca cagaggtgtt
     5521 gatttcgcca tgccgcaggg tacgccagtg ctttcagtgg gtgacggtga agtggtggtt
     5581 gccaaacgca gtggcgcagc aggttattat gtggctattc gtcatggtcg cagctacacc
     5641 acgcgttata tgcacttgcg caagattctg gtgaaaccgg gacagaaggt gaaacgtggc
     5701 gaccgtatcg cgctttccgg taataccgga cgttcaaccg ggccgcatct gcactatgaa
     5761 gtatggataa accagcaggc cgtaaacccg ctgacggcaa aactgccgcg taccgaaggg
     5821 ctgaccggct ccgatcgtcg cgaattcctg gcgcaggcca aagagattgt gccgcagcta
     5881 cggtttgatt aattaacatc cattcgcagc cggtacgcag tcagtaccgg ctttttttat
     5941 ttggtgcggg gcaagttgcg ccgctacact atcaccagat tgatttttgc cttatccgaa
     6001 actggaaaag catggaaacg aaaaaaaata atagcgaata cattcctgag tttgataaat
     6061 cctttcgcca cccgcgctac tggggagcat ggctgggcgt agcagcgatg gtgggtatcg
     6121 ctttaacgcc gccaaagttc cgtgatccca ttctggcacg gctgggacgt tttgccggac
     6181 gactgggaaa aagctcacgc cgtcgtgcgt taatcaatct gtcgctctgc tttccagaac
     6241 gtagtgaagc tgaacgcgaa gcgattgttg atgagatgtt tgccaccgcg ccgcaagcga
     6301 tggcaatgat ggctgagttg gcaatacgcg gaccggagaa aattcagccg cgcgttgact
     6361 ggcaagggct gcagatcatc gaagagatga ggcgtaataa cgagaaagtt atctttctgg
     6421 tgccgcacgg ttgggccgtc gatattcctg ccatgctgat ggcctcgcaa gggcagaaaa
     6481 tggcagcgat gttccataat cagggcaacc cggtttttga ttatgtctgg aacacggtgc
     6541 gtcgtcgctt tggcggtcgt ctgcatgcga gaaatgacgg tattaaacca ttcatccagt
     6601 cggtacgtca ggggtactgg ggatattatt tacccgatca ggatcatggc ccagagcaca
     6661 gcgaatttgt ggatttcttt gccacctata aagcgacgtt gcccgcgatt ggtcgtttga
     6721 tgaaagtgtg ccgtgcgcgc gttgtaccgc tgtttccgat ttatgatggc aagacgcatc
     6781 gtctgacgat tcaggtgcgc ccaccgatgg atgatctgtt agaggcggat gatcatacga
     6841 ttgcgcggcg gatgaatgaa gaagtcgaga tttttgttgg tccgcgacca gaacaataca
     6901 cctggatact aaaattgctg aaaactcgca gaccgggcga aatccaaccg tataagcgta
     6961 aagatcttta tcctatcaaa taaaaaaaag cctctcaaat tattgagagg ctgtactcga
     7021 taaaaaatct caatgagtgt tcccactatt ttgagttttt aggttagtac gctattgagt
     7081 taatcgtagc gtgataattt caaaaggttt gaactcaaga ttgcaatatt gattgcaata
     7141 aaaactgtca tctaacttat tttcttgaag atcgacatat tgaacgtagt gataattaca
     7201 tacccacgaa agcatggctg aaatttctaa tccatgactc tcatataatc ttacgattat
     7261 accgtcgccg tcttcagcta attttattgt ttcgatgata acgctgttag tatcgatagc
     7321 aaaaaatgat agtgaagggg gtaattcacc cgggtgcggt ttgacaggga cgacctgtaa
     7381 gggatgattc attatatagg cttgattaat aacaccaccc acacggtaat ctccatgatg
     7441 aggaaataag gagaatatga atttttgttt accaatatct gcaaaattat taatcaggaa
     7501 attatcataa ttgtctgtct taacaatttc cccaggttta tgctgcgaac gcagcaaagt
     7561 taattcaagc gtctggtctt tgacacgata gccatatttg ttttttgcta ttaaagctat
     7621 gccagtttct cgatcagaaa ggtcgaccca gtgatgtgct ggtatttcat ctttggcaaa
     7681 agcgaacgaa tcatcgctat gcgttggtct gtcaattaca ccgaactgaa tttgacattg
     7741 tgcaagagat gtttgtactg acaccgggaa tttgactttg agagaaacat taggttcgtg
     7801 ccaatcaata aaagtttgga catcaacgcg tggactgtcc tgtgtcaggg taaatgcgaa
     7861 gcgaagataa gaggaattgt agtgataaat cagcgtcatc gtagttttag gcccgctgat
     7921 tacagttgtc attttttcag cgagtaaaac atatgattcc ccatcacgat aatttgaagc
     7981 aaaatcccag gccgcgaaaa aacctgcatc ttcgtggtat gcacggatct cactgtgcat
     8041 attttcagta atgtattctt taccatatct tttatcatat agcgaaatta aatcaccgtt
     8101 ttctgaaaaa agaagtttaa tgtaattatt ttcaatactt cgagattctg ccttcagacc
     8161 atttacgatc ttattctttg gatcaatcac agcataacca taagagttta ctcgtgcttt
     8221 gagccagttg ttgtttatat tgatccattc gttacgagca aaacaggtgg tattaaataa
     8281 aatgtgcgga tctttataag aagaagtatc aattttactt aataaagtag actgcgtatc
     8341 agaaataatt ttctcggttt ttgcttctag tttaatatat tccttttcag tttcaagata
     8401 aactcttgaa atacatgatc cgggaaggat gtcatggaat tgcaaggtca gcatctcttt
     8461 ccatatttca tcaaactcac tgcgaagaat tgatgtcata ttattagtaa tagttgtaaa
     8521 aaattcagca tcatgtaatt tattttccat atttcgatta tgagctttag ttgctgactc
     8581 tgatgtaaag cacccttgat gagcctcgaa ataaagttca cctgaaataa tgggcaagct
     8641 ttcgcgaaaa gcgtcggcgt aagtgaagaa cttatcaaca cggctgaaat caacatgagg
     8701 taagccatca ata
//
LOCUS       DAJVUJ010000168         8598 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.168, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000168 DAJVUJ010000000
VERSION     DAJVUJ010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8598
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.168"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..183
                     /locus_tag="OQQ48_003566"
     CDS             <1..183
                     /locus_tag="OQQ48_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HCS1954198.1"
                     /translation="EAIKRLNKLRGWRKVSRSAILREAVEQYLERQQFPVRKAKGGRQ
                     KGEVVGVDDQCKEHKE"
     gene            402..644
                     /locus_tag="OQQ48_003567"
     CDS             402..644
                     /locus_tag="OQQ48_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1954199.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(974..1903)
                     /gene="fdhE"
                     /locus_tag="OQQ48_003568"
     CDS             complement(974..1903)
                     /gene="fdhE"
                     /locus_tag="OQQ48_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HCS1954200.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLDDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(1900..2535)
                     /gene="fdoI"
                     /locus_tag="OQQ48_003569"
     CDS             complement(1900..2535)
                     /gene="fdoI"
                     /locus_tag="OQQ48_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCS1954201.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(2532..3434)
                     /gene="fdoH"
                     /locus_tag="OQQ48_003570"
     CDS             complement(2532..3434)
                     /gene="fdoH"
                     /locus_tag="OQQ48_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HCS1954202.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(3447..6497)
                     /gene="fdnG"
                     /locus_tag="OQQ48_003571"
     CDS             complement(3447..6497)
                     /gene="fdnG"
                     /locus_tag="OQQ48_003571"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(5910..5912),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCS1954203.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVANYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            6691..7524
                     /gene="fdhD"
                     /locus_tag="OQQ48_003572"
     CDS             6691..7524
                     /gene="fdhD"
                     /locus_tag="OQQ48_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HCS1954204.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
     gene            7678..>8598
                     /locus_tag="OQQ48_003573"
     CDS             7678..>8598
                     /locus_tag="OQQ48_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HCS1954205.1"
                     /translation="MKRNLLSSAIIVAIMALGLTGCDDKKAETETPPPANSQPAAPAP
                     EAPVAKAEAKPETPAQPVVDEQAVFDEKMDVYIKCYNKLQIPVQRSLARYVDWLKDFK
                     QGPTGEERTVYGIYGISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAVALGNT
                     INEMDKYYTQENYKDDAFAKGKTLHQTFLKNLEAFEPVAESYHAAIQEINDKRQLAEL
                     KNIEEREGKIFHYYSLAVMISAKQINNLISQEKFDVDAAMKKVSELETLVAQAKEADK
                     GGMNFSFINSAGQYQLEAKKYVRRIRDKVPY"
BASE COUNT         2048 a         2176 c         2275 g         2099 t
ORIGIN      
        1 gaggcaatta aacggctgaa caagcttcgg ggctggcgta aggtttcacg atctgcaatt
       61 ttacgcgaag cggttgagca atatctggag cggcagcaat ttccggtgcg taaagcaaag
      121 ggaggcaggc aaaaggggga ggtagttggt gttgatgatc agtgtaagga gcataaggaa
      181 tgaaaaaaat ttttcattct tgaatataaa aaaacagatg cccttattct ggtattaata
      241 caaggctgtt ttacttgaac ttataataac tgcaactgtt acatcgtatc tggaaaacgc
      301 ctcgcaaaaa taaaaaatga tgcgtaaatg agctttcttt aaagattcgg caagggtact
      361 tttctctcca tagattcaaa aatgatattg gcgagatatt tatgaactct ctggcaggta
      421 tagatatggg cagaatttta ctcgatttat cgaatgaggt gattaagcaa cttgatgatc
      481 tcgaggtgca gcgtaatctt cctcgtgcgg acctattaag ggaagcggta gatcaatacc
      541 tgataaatca atcgcaaaca gcaagaacca gtgttcctgg catctggcaa gggtgtgagg
      601 aagatggtgt cgaatatcag cgtaagctgc gcgaggaatg gtaatgggaa aaaatgatgt
      661 caatcagatt gctgacaacg tgcgcgttgt tcatgccgga tgcggcgtga acgccttatc
      721 cggcctacaa agtcatgcaa attcaatata ttgaagttta ttggtaggcc tgataagcgc
      781 agcgcatcag gcaatcatac gcttgtcatc agtgacatac ccataacctt atcgatgtgt
      841 tggagggaag aacgaggcaa acagacaatt gcggcatgat cttgaatggg taacgcggaa
      901 tacgagagat ggtgtcgtca caccctatga gctgtaatcc gggcgaccag cgccacccgg
      961 aaaacgtcag agattactcc ccttcacccg gaaacaggaa cgggttgatg gaactgcggg
     1021 catagccttc ttgctccatt cgcgcgtcca gtaccagaga agcgaggtca tctgccacgg
     1081 cttcaacttt cggttctttt tcctgataaa gaatcttcag gtaagtgccg cagtcatcgc
     1141 agctttcggc tttaatcgcg gcctgttcgt catccagcga ccagtaatgc agtttgccgc
     1201 tctgttcaca gttgctgcat tttacgcgca ctacgtgcca ttcggtttca cacaggttgc
     1261 agtgcaggta acgcagaccc tgagtggtgc caatttgcac catgctggac accggcatag
     1321 aaccacaaac cgggcaatat tgacgttgct cgccgtattc agcgcgggct ttgccgggga
     1381 tcagattggc catctgcgcc cagtagagcg acagtgcagc ccagataaac ggcgctttat
     1441 cgctgctgac ggatgagaaa tcagaggcaa acagtgcgct ggccatatct tccagctcct
     1501 gagtcgatgc cttctccaga ttctcaatca ctgccagcgc cgggccgctc atttcaggtt
     1561 tcagctcagc aatcagcgcc atcagcagct tttgccagtg cttatcacgc ggcagaacgt
     1621 gaatatccag cgggggcttg ccctgtgcgc tggcttcttt aatgcgcgcg gtcagatcca
     1681 tctccagcgg atggtcgtac agcaccactt cctgggcgtg ggcgataagc gcagcaaagc
     1741 gcaggtaatc atccagcgga ttattttctg ccagctcgcg cagacgctcg gcgcggcggt
     1801 tgtataaatt cttgagccga gggaacaata acggcggaat catatccgcc gtacgtttct
     1861 cgctcgaacc cagctcatct tgcgggatta tgcgaatact cattcagctt tcttttccgt
     1921 tgtcttgcgg acctcacggt accagcgcgg gtgatgtttc ttcgcccagg cgctggtaac
     1981 ccatccttcc accatggcgg taatcgtgcc tttcacccaa agggcggcgt agatatgcac
     2041 catgataacc acaattaacg ccactgcggc aaatgaatgc agcattaacg cgaatcggat
     2101 caccgggatt gagaaagcag gcgcaaaata aggacgccag ataatcacgc cgctcaccag
     2161 cagcagaacc aggaaaataa tcgccgccca gaaaacgcat ttctgaccga agttataacg
     2221 cccggtgtca cctacttcct cgttgacgac gatcttacga atattcttcg cccaaaagat
     2281 atcatcccga ttgattaggt tgtgatgcca gtaacggaaa aacatgatga tgaacgaggc
     2341 aaacataacc acgccgacaa acgggtgcag aattcgcgcc agctgcggtg tgcccatgat
     2401 ttgcatcaac cagttgaagg acgggaacaa aaagcccagc ccgctcaccg ccgctaggat
     2461 gaagcagaag gcggtgatcc agtggttgat acgttccggc gcggtgtagc gcacgatggt
     2521 gtcacgtcgt ttcatttgcg ctcctcgtct ttctcttcgt gcagattatt ctcttcctca
     2581 tccgcacggt tcggaccgac acccacgtag tggaagatac tggctgcgaa ggtagccgca
     2641 aagccaacag ctgcgagcgg tttccagatg cctttccaga atttcacggt ttcgctgatt
     2701 tccgggttct ccggcaagcc atgatacaga tttggcttgt cagcatggtg cagcacgtac
     2761 ataacgtgtg taccaccgac gcctgccgga tcgtacaggc ccgcattgtc gtaaccgcgg
     2821 gttttcagct ccgccacgcg ctcgctcgcc agcgttttca tcgactcttt cgtaccgaag
     2881 tgaatcgcgc ccgttgggca ggtcttcacg caggctggtt cttgcccaac caccacgcgg
     2941 tcaacgcaca gcgtacattt gtagacgcgg ttgtcttccg ggttgaggcg cggaatgtcg
     3001 aacggacagc ccgcaatgca ataaccgcag ccaatgcact gctcggactg gaagtcgacg
     3061 ataccgttgg catactgaat aattgcacct tccgccgggc acgctttcag acagcctgga
     3121 tcggaacagt gcatacagcc gtctttgcgg atcagccatt ccagtttgtc gttctgctcc
     3181 acttccgaga agcgcatcac cgtccacgat ttggcactta aatcattggg gttgtcgtac
     3241 accccaatgt tattgccgac ggtatcgcga atgtcgttcc actctgaaca cgccacctga
     3301 caggctttac agccgataca ggtggtgacg tcgatgagtt tcgccacttc ttcctggaag
     3361 tcccgcgcct gaggcgcggg ggtcagaccg ttagtcgcgg aacgacgaat gatatcttga
     3421 gattgataag ccatatgtcg tctccgttac accttttcca cattcacaag gaaggactta
     3481 aactccggcg tctgcgtgtt cgcatcacca acgaatggcg ttaaagtatt ggcaataaag
     3541 ccttttttag ctacgccttc atagccccag tgaatcggaa taccgatggt atcgatatct
     3601 ttgccgttcg ctttcagcgt gcgaatacgt ttggtcacca ccgctttggc tttgatatag
     3661 ccacggttgg aggagacttt cacggtatcg ccctgggcaa tgccaagttt attcgccagc
     3721 gactccccga tttccacaaa ctgctctggc tgcaaaatcg cgttcaacag cgcgtgtttg
     3781 gtccagtagt ggaagtgctc ggtcagacga taggtggttc cgacatacgg gaacttatcg
     3841 gctttaccca atgcttcggc gtcgtcttta aagatacgtg cagccgggtt cgagataacg
     3901 tttggatgca gcgggttagt tcccagcggc gtttcaaacg gctcgtagtg ttccgggaac
     3961 ggaccttccg ccatcttatc gagggcaaac agacgcccca tgccttcctg ctgcatgata
     4021 aacggcccga cgccgctacc cggaggcgct gcgctgtagt ccggaatatc ccagccggtc
     4081 cacttagtgc cgtcccattt cagcaactga cgcttcggat cccacgggtt accctgcgga
     4141 tctgcggagg cgcggttata cagaatgcgg cggttaagcg gccatgccca tgcccagccc
     4201 agcgtgttac cgaggccaga cggatcggcg ttatcacgac gcgccatctg gttgccttcc
     4261 ggcgtccagc taccggcgaa aatccagcag ccacaggagg ttgtaccgtc atcgcgcaat
     4321 tgggcgaacg agctaagttg ttggcctttc ttgacgataa ccgcaccggt tgccggatcg
     4381 gtaatatcgg ccagcgcctt accgttgctc tccatcgcta cttcttccga cttcggctca
     4441 tgcggaatgg cgtagttcca ggtcatgttc agcacctgat ccgggttcgc gccaccctgt
     4501 tcggcataca tcttgcgcaa gcgcaggaag ataccggaga ggatctcacc gtcagtcagc
     4561 gcaatccccg gggcgtccgc acctttccag tgccactgca accagcgacc tgagttaacg
     4621 attgaaccgt tctcttctgc gaagcaggtc gatggcagac ggaacacttc ggtctggatc
     4681 ttcgacgagt caacttcgtt cagctcaccg tggttctgcc agaagttaga ggtttcagtg
     4741 ttcagcgggt cgatggtgac gaggaacttc agtttcgaca gacaaccgat cactttgttt
     4801 ttgttcggga atgaggcaac agggttaaaa ccctggcaga tatagccatt gaccttgccc
     4861 tctttcatca tctcgaagta ttgcaggacg tcgtagcctt tatcccactt cggcaaccag
     4921 tcaaagcccc agctattttc cgccgtcgct ttatcaccaa agaaggcctt catcatagag
     4981 acgaagaatt tcgggtagtt gccccagtag tttacctggc cttccagcag cggttttggc
     5041 gtgttggcgg taaggtaggt ttgcagatcg gtctgcttct cgcttggcag cgtcatgtaa
     5101 cctggcaggc tctgcgacag cagccccagg tccgtcagcc cctgaatatt ggagtgaccg
     5161 cgcagggcgt taacgccgcc gcctgccatc cccatattgc cgagcagcag ctggatcatc
     5221 gccatcgtac gaatgttttg cgcaccaacg gagtgttgcg tccagccgag ggcatacagg
     5281 aacgaggcag ttttatcgtg agcactggtt tctgcgatgt attcgcagac tttcaggaac
     5341 gcgtcttttg gcgtaccaca gatgttttca accacatctg gcgtgtagcg ggaaacgtgc
     5401 tgtttcagca agttccacac gcagcgcgga tgttgcagcg tggtatcgcg tttggcgaag
     5461 ccgttttcgt ccagttcgta agtccaggag gatttatcgt acttgcgttt ttccgcgtca
     5521 tagccggtga aaaggccatc ttcaaagccg taatcctcac gcacgatcag gctggcgttg
     5581 gtataggctt cggtgtattc gcggttgaat ttttcattgt tcagcaggta cagcaatacg
     5641 cctgacagga aagcaatgtc agtaccggaa cgaatagggg catagtagtt agctaccgcc
     5701 gccgtacgcg taaagcgagg atcgatcaca atcagcttcg cgccgttgtg aattttggct
     5761 tccatcgccc agcggaaccc gaccgggtga gcttcagcgg cgttaccgcc catcaccacg
     5821 acgaggttgg cgttcttgat gtcgacccag tggttggtca tcgcaccgcg accaaatgtt
     5881 ggagcaagac ttgctaccgt tggtccgtgt cagacacgcg cctggttgtc gaccgcgagc
     5941 atacccagcg cgcgggagaa tttttgcgtt aaatagccgg tttcgttact cgacgcggaa
     6001 gcacacagca tcccggtgga gagccagcgg ttaacagtca cgccttcggc gttttgcgca
     6061 atgtagttag catcgcggtc ttctttcatc agtttggcga tgcgatcaaa cgcctcttcc
     6121 caactgattt gctgccattt atcagaacct ggcgcacggt attccggaaa cttcagacgg
     6181 ctttcggagt ggatgaaatc caccaggcca gcgcctttcg gacaaagtgc accgcggttg
     6241 accgggtgat ccggatcgcc ttcgatatgg aagatagatg ctttggcgtt ttttgctccg
     6301 tcaccgaggc tgtacatcaa cagcccacag cctacggaac aataggtgca ggtattacgg
     6361 gtttcgcggg tgcgcagcag tttatactgc cgggtttccg cgagtgctac gctgggtgca
     6421 aaacctagtg ccgctgccgt ggtgcctgcc ataccgccag cgcagatctt aaagaactgc
     6481 cttctgctga cctgcatgga ttgctccttg tttcgacatt gtcacgtccc atttacattc
     6541 gcttgctgcg tgtgcaggga gtgggagtta tttttctttg cggaaggggc cgcaaaggtc
     6601 cagaattggc tcgatttccc tccatccagg aagggtttgt aacagaatac cataatgttg
     6661 gtgtgtgtgt tcttatctgg ttaagagaaa gtgaaaaaaa cacagcgaaa agaaatcgaa
     6721 aatgtgacaa atatcacagg tgttcgtcaa attgagttat ggcggcgtga tgatttacaa
     6781 cacccacggt tagatgaggt cgcggaagaa gttcccgttg cgctggtcta caacggcatt
     6841 tcgcatgtgg tgatgatggc gtcgcccaaa gaccttgagt actttgcgct cggtttttcg
     6901 ctttccgagg ggattatcga aagtccgcgc gatatcttcg gcatggatgt cgttccttcc
     6961 tgtaatggtc ttgaagtaca aattgagctt tccagccgcc gctttatggg gttgaaggag
     7021 cgccgccggg cgctggcggg acgtacgggc tgtggcgtat gcggcgtgga gcaacttaat
     7081 gacatcggaa aaccggtgca gccgctaccg ttcacccaga cgtttgatct caacaaactg
     7141 gatgatgcat tacgtcatct caacgatttt cagccagtgg ggcaactgac tggttgtact
     7201 cacgccgctg cctggatgtt gccatctggc gaactggtcg gcgggcatga agacgtgggg
     7261 cgccatgtgg cgctggacaa actgttaggc cgccggtcac aagaagggga aagctggcag
     7321 caaggtgcgg tactggtttc cagtcgtgcc agttatgaaa tggtgcaaaa gtcggcgatg
     7381 tgcggcgtag agattttgtt tgcggtgtct gccgcgacca cgcttgctgt agaagtggct
     7441 gagcgctgta atctgacact ggtaggtttt tgtaaaccgg gtcgggcaac ggtttatacc
     7501 catccgcagc gtttgagcaa ttaattaaaa taagtcctgc gatatatatt aaattgcagg
     7561 acttattcat ttcgtgaatt ttattatttt tatttataaa taaaacgcgt gtagtggcgt
     7621 tttctttttt catttattat gttcactctc ttatctatat atttataagg caattaaatg
     7681 aaaaggaatt tattatcttc cgctattata gtcgccatca tggccctcgg tctgacgggt
     7741 tgtgatgata aaaaagccga aacagaaacg cccccgcctg ccaatagtca acctgccgca
     7801 ccagctcctg aagctcccgt tgcaaaagca gaagctaaac ctgaaacacc tgcgcaaccg
     7861 gtggtcgatg aacaagcggt tttcgacgaa aaaatggatg tctatatcaa gtgctataac
     7921 aagttacaga tccctgtaca gcgcagtctg gcgcgttatg ttgactggct gaaagatttt
     7981 aaacaggggc ctaccggtga agagcgtact gtttatggca tctacggcat tagtgaatcc
     8041 aacctcgctg agtgtgaaaa aggcgtaaaa agtgctgtgg cgttaacgcc tgcgctgcaa
     8101 ccaattgatg gcgtggcggt gagttatatc gatgctgccg tggcgttggg taataccatt
     8161 aacgaaatgg ataaatatta cacccaggaa aattataaag acgatgcgtt tgcgaaaggg
     8221 aaaacgctgc accagacatt cttaaaaaat ctggaagcct ttgaacctgt agcagaatct
     8281 tatcatgcgg cgattcagga aattaacgat aagcgccagc ttgcggaact gaaaaatatt
     8341 gaagaaagag aagggaaaat attccactac tattctctgg cagtcatgat ttcagcgaaa
     8401 caaattaata acctgatatc gcaagagaag tttgatgttg atgcggcaat gaagaaagtg
     8461 tctgaactgg aaacgctggt ggcgcaggcc aaagaagcgg ataaaggcgg catgaatttc
     8521 tcgtttatta attcggcagg ccagtatcaa ctcgaggcta aaaaatacgt tcgccgcatc
     8581 agagataaag tcccgtac
//
LOCUS       DAJVUJ010000169         8478 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.169, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000169 DAJVUJ010000000
VERSION     DAJVUJ010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8478
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.169"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(313..891)
                     /gene="mocA"
                     /locus_tag="OQQ48_003574"
     CDS             complement(313..891)
                     /gene="mocA"
                     /locus_tag="OQQ48_003574"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HCS1954206.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYVNQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIKRPNITNMRQALLMG
                     AHYSVEVENAEIILDIDTPDDFITVQKRYTKI"
     gene            994..1764
                     /gene="yqeC"
                     /locus_tag="OQQ48_003575"
     CDS             994..1764
                     /gene="yqeC"
                     /locus_tag="OQQ48_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HCS1954207.1"
                     /translation="MKSIVDPSALFIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRAMPLKAPDEHEPCIPKSSCCVIAVMGGHILGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            1812..3437
                     /locus_tag="OQQ48_003576"
     CDS             1812..3437
                     /locus_tag="OQQ48_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HCS1954208.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(3478..4410)
                     /gene="arcC"
                     /locus_tag="OQQ48_003577"
     CDS             complement(3478..4410)
                     /gene="arcC"
                     /locus_tag="OQQ48_003577"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCS1954209.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNVLREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(4458..5843)
                     /gene="hyuA"
                     /locus_tag="OQQ48_003578"
     CDS             complement(4458..5843)
                     /gene="hyuA"
                     /locus_tag="OQQ48_003578"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HCS1954210.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(5896..7107)
                     /locus_tag="OQQ48_003579"
     CDS             complement(5896..7107)
                     /locus_tag="OQQ48_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HCS1954211.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQIYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(7165..8361)
                     /gene="dpaL"
                     /locus_tag="OQQ48_003580"
     CDS             complement(7165..8361)
                     /gene="dpaL"
                     /locus_tag="OQQ48_003580"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HCS1954212.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
BASE COUNT         1997 a         2061 c         2218 g         2202 t
ORIGIN      
        1 cttagcttat acagatatca ttgagtgacc ggtgttgaga aatccggtgg tttaggggga
       61 tagagcaaca atcgcaccac tcttgccagg tttggcggga aatataaaaa aatgtgaggc
      121 ggctcgaaac ttaagggctt tttgtgaatg aactcacagg tgctaaatga gaattaaaat
      181 cactcctgaa aattattatt gccagcccat atatcacttt ttctcttttc tcaaaatcag
      241 tgattcctta tcattatgat aaagagtgct ggagggatta aatatttgaa cgcaatcgtt
      301 taactacgtt ttttaaattt tagtatacct tttttgcact gtaataaagt cgtccggagt
      361 atcaatatct aaaattattt ctgcattttc tacttcgacg gaataatgag ctcccataag
      421 gagtgcctga cgcatattgg tgatattggg gcgcttgatt gcctgcatca ggcatggttt
      481 tgacactaaa atcggatggc cggggatgcc attatggagt ggcagtattg cgccatcatt
      541 tcgtaacgac cagatttttc tgaaaatatc gatggtgagg gttggcatgt caccgtgggt
      601 gagaaaacaa tgttctgttt gcactgcggg tactgcggcc tttactgatg ttagtaaacc
      661 ctgcgcataa tctgggttgt ggataatagt gatattgctc tggttcacat aacgttcgtg
      721 cagttcatta ccacgatagc cggtgactaa aataattcgg ctacaaaact gcaacgcatt
      781 tttgatactt gtatcaagaa ttgtcccctg ttgccagggt aacatcattt tccattgccc
      841 cattcttgat gataatccgg cagcggtaat tatacagtcg atggctgaca tagtggttcc
      901 attatttata ggggctaata acgcctgata attgcgttac ttcctgatgg attaagttgg
      961 ggataattta tcccagagag gtcataaaga ctcgtgaaaa gtatagttga cccatcggca
     1021 ttattcattg atttaggtgc gcagaaacgt cccactgtga tttctgttgt tggtgctggg
     1081 gggaaaacca gcttgctttt ttggctggca gaactgctcc aggctagcgg caggcgtgtg
     1141 ttaatcacga cgacaacaca tatgtttatg ccaacgtctc actggcccgt ggttttctgt
     1201 cgtgatcccg ccatgcttcc tcatgcgtct cttacatccc ccatttcatt ttgttttcac
     1261 agctggaaag cgaaccaggg aaaagtgcag ggatttacgc cagaagcgat tgatgcactg
     1321 gtgcaacgac cagagtgtga cgtaattctc attgaagcag atggctcgcg tgcaatgccg
     1381 ttaaaagcgc ctgatgagca cgaaccttgc atacccaaaa gcagttgctg cgtgattgct
     1441 gtgatgggag gacatatttt gggcgcgaaa gtgagcacag aaaatgtcca tcgctggtcg
     1501 cagtttgctg atattactgg gttaacacct gatgcaacct tgcaactgag cgatctcgtt
     1561 gcgctggttc gccaccctca gggggcgttt aaaaacgtac cgcaaggttg tcggcgagtc
     1621 tggttcatta accgtttttc tcaatgtgag aatgcgattg cacaaagcga gctccttcaa
     1681 ccgctgcaac aacacgacgt agaggcaatc tggctaggcg atatacaaga acatcctgca
     1741 atcgcgcgca gatttgtgaa ttagcggccc gggtattccg ggttactgaa aggatatcca
     1801 ttgaggttgg tatgaatatt ttcacagagg ctgcaaaact cgaagagcaa aattgtccgt
     1861 ttgcgatggc gcaaattgtt gatagccgag gctcgactcc ccgccattct gcacaaatgt
     1921 tagtgcgcgc cgatggttct atcgtcggta caattggtgg cggaatggtt gagcggaagg
     1981 tgattgaaga gtcacttcag gcattgcagg aacgtaagcc gcgattattc catggacgta
     2041 tggctcgtaa cggtgcggat gctgtcgggt cagattgtgg aggtgcaatg tcagtattta
     2101 tcagcgtcca tggtatgcgt ccacgtctgg tgttgattgg cgcggggcat gtcaaccggg
     2161 cgatagccca gagtgccgcg ctattaggat ttgatattgc cgttgccgat atttatcgcg
     2221 aaagcctcaa tcctgaacta ttcccaccat caaccacgct tctccatgct gagtcgtttg
     2281 gtgcggcagt ggaagcactg gatattcgcc ctgataattt tgtcctgatt gccacgaata
     2341 atcaggatcg tgaagccctc gacaaactca ttgaacagcc cattgcatgg ttggggttgc
     2401 tggcaagtcg ccgcaaggtt cagcttttcc tgcgtcaatt gcgtgagaaa ggcgtggctg
     2461 aagaacatat tgcccgttta catgcgcccg ttggttacaa cataggtgcg gaaacgccgc
     2521 aggagatcgc catcagcgtg ctggcagaaa tattacaggt gaaaaataac gcgccgggtg
     2581 ggctgatgat gaaaccttct catccttccg gacaccagct ggtggtgatt cgcggtgcgg
     2641 gagatatcgc cagtggtgtg gcgctacgtc tgtatcatgc gggttttaaa gtgatcatgc
     2701 tggaagtgga aaaaccgaca gtgattcgtt gtaccgtggc gtttgcccag gccgtgttcg
     2761 atggcgaaat gacggtcgaa ggcgtcactg ctcgcctggc aaccagctct gcggaagcga
     2821 tgaaacttac cgaacgcgga ttcatccctg tgatggtaga tcccgcctgt tcattgcttg
     2881 atgaactgaa accgctttgc gtggtggacg ctattctggc gaaacagaat ttgggaacgc
     2941 gggcagatat ggcaccagta acaatcgcgc ttgggccggg ctttactgca gggaaggatt
     3001 gtcatgcggt aattgaaaca aatcgcgggc actggctcgg tcaggtgatt tactctggtt
     3061 gtgcgcagga gaataccggt gttcctggca atattatggg gcataccacc cgacgggtga
     3121 tccgtgctcc tgctgcaggc attatgcgat ccaacgtgaa attaggcgat ctggtgaaag
     3181 agggcgatgt gattgcctgg attggtgagc atgaaattaa agcaccgttg acggggatgg
     3241 tgcgtggctt gttgaacgac ggcctggcag tggttggtgg ttttaaaatt ggtgatatcg
     3301 atcctcgtgg tgaaaccgct gatttcacca gcgtttctga taaagcccgg gcgattggcg
     3361 gcggcgtact tgaggcgtta atgatgttga tgcatcaggg cgtgaaagcg acaaaagaag
     3421 tgctggaagt ggcttaaatg aaaatggccg gatggtatta cccacccggc cttaaaatta
     3481 ctgacaaatc accgttccgg ttttgccttc aatcccttct ttcgctttgc tcaatacggt
     3541 aatcagtgct tcacgacctg cgcgagagcg agcaaacgag gcggcagctt ccactttagg
     3601 cagcatagaa cctttcgcaa aatgaccttc ttcgataaag cgttcagcat cacttaatga
     3661 caggcgatcg agccactgtt cattctcttt accgaagtta atggccactt tttctaccgc
     3721 agtcagaatg atcagcatat cggcatcaat catttctgct aaacgggcgc tggcccagtc
     3781 tttatcgata accgcgctgg caccacgcag atggttacct tcacgaataa cagggatacc
     3841 accaccgcca acggtgatca ccacctggcc tgcatctacc agagctttaa cagtttcttt
     3901 ttcaatgata tcaaccggtt ttggcgaggc aacaacacgg cgatagccgc gaccagcatc
     3961 ttctttcaga gtgtaaccct gttttgtcag ttgctctgct tcttgctcgg taaagaacga
     4021 accgatcggc ttggtcgggt tgaggaatgc cggatcgtta gcgtcgactt cgacctgagt
     4081 aaccagcgtt gctacaggtt tattaatgcc ccgagaaagc agttcttctc ttaacacgtt
     4141 ttgtagatcg taaccaatat aaccctggct taaggcaaca cagacagaca tcggcagcat
     4201 tggcgagtgt gcttcagttt ttgcggcagc ttcaaaggcc tggttaatca tgcccacctg
     4261 cggcccgtta ccatgagtga cgataacttc atgtccttgg gcaattaaat caacaattgc
     4321 ctgagaagta atttttaccg ctttcatttg tccggccagg tcatcgccca gcgcattccc
     4381 gcccagggcg agaacaattt tcttactcat ttttcctcac tcatcattct tgattgtgta
     4441 tagatccctg acagtggtta gagcacggga gggacaaacg gtttgcgtcg caggaaacga
     4501 cctcgcccgg ctttgcctgt aaaggtgccg tcacagaaaa tcgtttcacc acgggataag
     4561 gttctgacaa tcgcgccctg acaggtaaaa ccctcccatg gcgagtagtc ggcgttgtcg
     4621 tggagatggc gatgctgaat ttgttggctc tgacgtgggt cgataatcac cacgtcgcca
     4681 tcggaaccgg gcgctaataa tcctttttgc ggccacaggc caaacagcct ggcgggcatt
     4741 gcgctggtta attcaacaaa gcgttccggt gttatacgtc ccgtcatcac gccactggaa
     4801 aacagtaact gcatgcggtt ttccacaccg ggtaagccat ttgggcagcg actgaaatcg
     4861 cctttagaaa tttgcaggcg ttgagccatc gagaaggtgc agtgatcggt cgccaccacg
     4921 tcaatcgcac catcgctgat gccacaccac agtttgtcct gctcgcgtac gttacgcagc
     4981 ggtgggctaa gaatgaactt catgccatct tctgtatcgt aactgcgttc gtccaacagg
     5041 agatattgtg ggcaggtttc aacccagact ggctggtgat tcgcacgggc aagacgcaga
     5101 taatccagac ctaagccgtt agacaggtgc acgatataga gcggggcgtt accggcaatt
     5161 tgtgccaggt taatcatgcg ggcgatggct tccgcttcgc attccagagg gcgactcaag
     5221 gcatgatagc gcggcgcggt taaccctgcg gcgataaatt ccgcccgctt gctggcgata
     5281 gccgcatcat tttccgggtg cacggtggtc agcgcgccgg attcatgcaa gcggcgtaat
     5341 gcctgcaaaa cctcgtcatc gttgagtttg tattgatagg ttaaatagag tttaaaactg
     5401 ctcagtcctt cctcgaccat catcgggatt tcgtcgagga ttgcgtgatt aatgtgctgg
     5461 atcacaccgt gaaagctgta atcgatgacc gctttatggg cggcataacc acgataaacc
     5521 tccagttgat ggcgtaaccg acagccgttt gggccaaatc ccatatggtc aataatggtt
     5581 gttgtaccgc cacacgcagc tgcgcgggta ccggtaaaaa aatcatcaca actgcgcgcg
     5641 atgccgacat caatattgaa atgcgtatgg acatccacgc cgcctgggaa aacgtaacag
     5701 ccagtggcat caatttcttc atacgggagc tgcggcgaaa tattgttgcc caactggcga
     5761 acaatcccgc tttcaatcag caaatcctgt ttggcttgtc catctgcgtt aacgacagtg
     5821 ccgtttttga tcaatacgcg catagcaaac tccaaaaacc ctccggcgaa ccggagggga
     5881 ataagtgaag agtaattact ctgttgccag ccagcttaac gggattgcag cgtacatcgc
     5941 ggcacaggtc accaggtgag atttccaggt tttttcgtta ggtgcatgcg cttcaggttc
     6001 tttacccggg ccaaagccga tcaccggaat gccgtgacgg cccatgatag atacgccgtt
     6061 agttgagaag gtccacttat caacaaccgg cgctttgcca aacagccctt cgtaggcatt
     6121 caccagtgct ttaacggtga agtgatcttc ttccactttc caggtcggga agtagcattc
     6181 ggttgggtaa accaggccag tccaggacgg acggtcgtag ttgtacatag aaacaaccgc
     6241 gttagctttc tgtactgcag gcagggcgcg gatttcgtcc agcgcgcctt cccaggtttc
     6301 gccccaggtc agacggcggt caatagagac ggcgcagcta tctgctacag cgcaacggct
     6361 tggggatgtg aagaagattt cagaaacggt gagggtgcct ttgccgagga attcgtcata
     6421 acccagacgt tgggagagtt cttgtaattc gccaagaatc ggacccattt tgaaaatggc
     6481 gttgtcaccg cgttctggcg cagaaccgtg gcagctaaca ccctgaacat caatacgaat
     6541 ttccatacga ccgcgctgac cacggtatat ctggcagtcg gttggttcgg tactgaccac
     6601 aaattccggg cgaatgccgg attgttcaat aatgtactgc cagcacagac cgtcgcagtc
     6661 ttcttcctgc acagtaccgg taaccagcag ggtatattca tcttccagac cgaggtcttt
     6721 aatgatttta ccggcataaa ccatagatgc catgccgcct tcctggtcgg aagtaccgcg
     6781 accaccgatt agctcatcag tttccatgcc ttcgtacgga tcgaagtccc agtttttgat
     6841 gttgccaatg ccgacggtat cgatatgagc gtccattgcc accagacgcg ggccgtggcc
     6901 gatataaccg agaacgttgc ccatcgggtc gatttcaact ttatcgaagc cgactttttc
     6961 catctcttct ttaatacgat gtactacgcg tttttcgtcg cagctttcac tgggaatagc
     7021 aaccatgtcg cgcaggaagc gagtcatgtc cgcctggtaa tcttttgctt tttcaagaat
     7081 cagtttgaat ggaatattct tagccattat ttttctccag attttttact ccccgttttg
     7141 cagggagtag ttccaatagg gtgattaagg tgctacagcg tgtttgcctt cccagacaac
     7201 ttcgcggtag tgcttcacgt cggtgtcgcc ttcagtactg ataaccagca ctacggcatc
     7261 tttgttcagc gccagttttt ccatcaggct ttgacgttgc gggtgataat gaaccgctgc
     7321 gagaacgccc aaaccgacag cgccggattc accggagatg atgcgcgggt cgttgccgta
     7381 cggattaccc agcacgcgca tacctaatgc ggcaacgctg tcctggcagg agatgaattg
     7441 ggtggcgcag ttacgtagga tttcccagcc cagcgggtta ggttcgccac aggccaggcc
     7501 cgccatgatg gtggccatat caccgccaac gttgacgatg tcgcctttga cgccggagcg
     7561 ataaatacag tcagctttgt caggttcaac aataatgctg tgcagatttt gcgggctgta
     7621 gacgtcgacc agataaccca gcacaccacc ggccattgct ccgacaccgg cttgcagcag
     7681 aacgtgcgtc ggggttacgc ccatttcacg catttgctcg acggcttcat ctgccagggt
     7741 tgcgtagcct tgcatgatcc aggttgggat tttggtgtaa ccttcccatg ccgtgtcctg
     7801 taccacttcc cagccgtgct gctgcgcgtg ttgcatggtc aggcgaacgg tatcgtcata
     7861 gttcatatcc gtgacgatgc actcggcacc gaggttcaga atggcgtcaa cgcgttcctg
     7921 agcagaacct ttcggcatgt aaatcaccgc attctgtccg agttgctgtg ctgcccacgc
     7981 cacaccgcgc ccgtggttgc cgtcggtggt ggtcgcgaaa gtcatttttt cgccgatggc
     8041 atttttcagg tgctcaaatg acagcgtttc gatatcaaga tgatattttt cacacaataa
     8101 ttgagcgatg gcgtacgcac cgccaagcat tttgaaggcg ttcagaccga atcgttttga
     8161 ttcgtctttg acgagaattt tcttcacacc aaaaaggttt gcgagatcgt ccagcgcaca
     8221 aagcggtgtt gggcgataac cagctatttt ctggtggaac tggcgcgcca gtttggcctg
     8281 gctttgcgaa aagagcggtg atgtttcgcc gttgaaaaat ttgttatcgg cgatatcaat
     8341 cttcaatgag aaaacggaca tatcctatcc ttttggcctc tggaaataga gggccgacca
     8401 gctgatcggc ccgaggggtt atttcacgcg ttcttgcgct tctttcagca gttgctcgag
     8461 cagtgcacct ggtttggc
//
LOCUS       DAJVUJ010000170         8455 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.170, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000170 DAJVUJ010000000
VERSION     DAJVUJ010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8455
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.170"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..201
                     /gene="csgC"
                     /locus_tag="OQQ48_003581"
     CDS             <1..201
                     /gene="csgC"
                     /locus_tag="OQQ48_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="involved in autoagglutination of curliated cells;
                     not involved in production of curli fibers; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HCS1954213.1"
                     /translation="SLREGGSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIV
                     VTVSDGQALHLSQQWPPSSEKS"
     gene            322..633
                     /locus_tag="OQQ48_003582"
     CDS             322..633
                     /locus_tag="OQQ48_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCS1954214.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQVASVKVQYIDKQKKLAVMNIEY
                     N"
     gene            729..1262
                     /gene="ymdB"
                     /locus_tag="OQQ48_003583"
     CDS             729..1262
                     /gene="ymdB"
                     /locus_tag="OQQ48_003583"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HCS1954215.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            1204..2685
                     /gene="clsC"
                     /locus_tag="OQQ48_003584"
     CDS             1204..2685
                     /gene="clsC"
                     /locus_tag="OQQ48_003584"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HCS1954216.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRQMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(2693..3850)
                     /gene="mdoC"
                     /locus_tag="OQQ48_003585"
     CDS             complement(2693..3850)
                     /gene="mdoC"
                     /locus_tag="OQQ48_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HCS1954217.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMLVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            4244..5779
                     /gene="mdoG"
                     /locus_tag="OQQ48_003586"
     CDS             4244..5779
                     /gene="mdoG"
                     /locus_tag="OQQ48_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HCS1954218.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGGAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            5772..8315
                     /gene="mdoH"
                     /locus_tag="OQQ48_003587"
     CDS             5772..8315
                     /gene="mdoH"
                     /locus_tag="OQQ48_003587"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HCS1954219.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKHWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
BASE COUNT         2134 a         2005 c         2211 g         2105 t
ORIGIN      
        1 tccctgcgcg aaggcggttc agggcaaagt cagacgaagc aagaaaagac cctctcattg
       61 cctgctaatc aacctattgc tttgacgaag ttgagtttaa atatttcccc ggaagatcgg
      121 gtgaaaatag ttgttactgt ttctgatgga caggcacttc atttatcaca acaatggccg
      181 ccctcttcag aaaagtctta atttgttgaa atgtcgagca taagatgaat ctggagagaa
      241 tggtctgctg cgaatcagcc aacctgaaag tatggataac acaaccctca aggatgacta
      301 atcattgagg aaatagaata aatgttcaga ccttttttaa actctcttat gctcggcagt
      361 ttgttttttc cttttattgc catcgcagga agcaccgcgc aagggggcgt gatccatttt
      421 tatggccaaa ttgtggaacc ggcatgtgac gtcagcaccc agtcatcacc cgtagaaatg
      481 aactgcccac agaatggttc tgttccggga aaaacctatt ccagcaaagc gttaatgagc
      541 ggcaatgtca aaaacgcgca agtagcctca gtaaaagtgc agtatattga taaacagaaa
      601 aagctggctg taatgaacat cgaatataac taagtttctg aacaacgcac gttgctgagt
      661 agaaaaatgc gatttaacca aaaagcctgc tgtacactta agaaacaaga aggtgttaag
      721 gaggcgttat gaaaacgcgt attcatgttg tgcagggtga tattaccaaa ctggccgttg
      781 atgtgattgt gaatgcggct aatccgtcat taatgggagg cggcggcgtc gatggggcca
      841 ttcatcgcgc agcgggtccg gccctgctgg atgcctgttt aaaagtcagg caacagcagg
      901 gcgattgccc tacggggcat gccgttatta cgcttgcagg cgatcttccc gctaaagccg
      961 tagtacacac cgtcgggcca gtctggcgtg gtggtgaaca aaacgaagac cagcttttgc
     1021 aggatgccta tctcaatagt ctgcgactgg tggcggcaaa cagctatacg tcagtggcat
     1081 ttcctgcaat cagtactggg gtttatggtt accctcgtgc ggcagcggct gaaatcgcag
     1141 taaaaaccgt ttcagaattt attacccgtc acgctttacc cgaacaggta tactttgtct
     1201 gttatgatga agaaaacgcc cacctctacg aaagactcct tacccaacaa ggagatgaat
     1261 gatttgcccc ggctggcgag cgcggtgctg ccactgtgtt cgcaacatcc cggtcagtgt
     1321 ggtcttttcc ctctggagaa aagtctggat gcgtttgccg cccggtatcg tctggccgaa
     1381 atggcagagc atacgctgga tgttcagtat tacatctggc aggacgatat gtcgggtcgg
     1441 ttactgtttt ccgccctgtt agccgcagca aagcgtggcg ttcgcgtccg tttgttgctg
     1501 gacgacaaca atacgcccgg acttgacgat attttacgct tgcttgacag ccatccccgc
     1561 attgaagtcc ggctttttaa tcctttctcg tttcgcttgc tgcgtccgct tggttatatc
     1621 accgactttt cccgtctcaa tcgccgtatg cacaataaaa gtttcactgt cgatggcgtg
     1681 gttaccttgg tgggaggacg aaatattggt gatgcctatt ttggggcagg ggaagagcca
     1741 cttttttcgg atttagatgt tatggcaata ggacccgtgg tagaggacgt tgccgatgat
     1801 ttcgcccgct actggtattg caaatcggtt tcacccttac agcaggtgct ggatgtcccg
     1861 gaaggtgaaa tggcggatcg catcgagtta cccgcctcct ggcataacga tgccatgacg
     1921 catcgttact tacgccaaat ggaatccagt ccgtttataa atcatctggt tgatggaacg
     1981 ttgccgctta tctgggcgaa aacacgttta ttaagtgatg atccggcgaa aggggagggc
     2041 aaggcaaaac ggcattcact gttaccgcag cgcctgttcg atatcatggg ctcacccagt
     2101 gaacgcatcg atattatctc ttcctatttt gtaccgacac gcgcaggtgt ggcgcaactc
     2161 ttacggatgg tgagaaaagg ggtaaagatt gcgatcctaa ccaattctct tgccgctaac
     2221 gatgttgctg tcgtccatgc cggatacgcg cgctggcgca aaaaattgct ccgctatggc
     2281 gtggaattat atgaactcaa gccgacgcgt gaacaaagta gtacgttaca cgatcgcggc
     2341 ataaccggta attccggtgc cagcctgcat gctaaaacct ttagcatcga tggtaaaacg
     2401 gtgtttatcg gctctttcaa tttcgatcca cgttcgacat tgctcaatac tgaaatgggc
     2461 ttcgtgatag agagcgaaac gctggcacag ttaattgata aacgctttat acagagccag
     2521 tatgatgcgg cctggcagct ccgtctggac aggtggggac ggatcaactg ggttgatcgt
     2581 catgcaaaga aagagattat tctcaaaaaa gaacccgcca ccagtttctg gaagcgggtt
     2641 atggtcagac tggcgtcgat attgcccgtg gaatggttat tgtaaatgtg gcttaacggg
     2701 ctggtgcttt atcgttctca cgcttaacaa ccggtttacc agaaaacaaa aacttcagta
     2761 acgggatgcg taaatgaatt tcatacagaa ttatcgcaat ccctactacg aatatcaggc
     2821 cacagagaaa accaagccag ttggaggtga tgtgcggtgt aatgtatgcg ccgaaaaaca
     2881 gcgttaacgg gtggtgaacc agatagataa atagcgatgc gttaacaaag taagtcaccc
     2941 gcgctgactg gaagttaagc aaacggtggc caaaggagaa gaccacattc accatccaca
     3001 gaccgaggac catggtgatc accgactcgg tttcgtacat ccaggcatcg ccactgccat
     3061 agcgttggtt gagtaaataa gcgacaaatg ccaatgctgc tgcaagggta cagccacgag
     3121 acggcgtggt aaacaaggct ttaagatgag ggaaaatgaa agccagtgca ccgaggataa
     3181 agaacggcaa ataaaatagc gtttgcatga caataaaatt gaacatgcca ttactcagaa
     3241 tgggcggata cacaataaaa atcgttcttc ttattaccgc atagccgatg ccgaggcata
     3301 aaaaaatcac cgatagtttt accatcgaga attttttatt cgttttatca gaattttcta
     3361 aattatttct gatgcgttta aatatccata cgcacagcgt cgtcatgact actaacacca
     3421 gtaaaaacca caggtgtgat attaattccc aggccaacgt attatatttg tcatacaatg
     3481 acagcccagg ccaactttcc gctttccctt tgacgtattg cagcataata aattgcggca
     3541 atgtcagtaa ggggatggct gttaacatcg ggatacctac acgttcgaca cgtactttcc
     3601 accatttttt caagggatag cgtaaaaaaa gcatgtagga aaagtagccg gatataacga
     3661 aaaataccag catgcggaac gagtggatga agtcattaaa aagggtcagc cacaatgacg
     3721 gttcggcgct attcacatgc catgtatggc tcgaatagat taaagaaata tgaaaaggga
     3781 tccctaacaa catcagccag gcgcggatgg agtcgaggaa atattcacgt tgcgcgggta
     3841 ctggattcat atatggttaa ctaatctcgg atttttcgtc ttatccctgt cgggttatgc
     3901 ctttaggctt gttgccatag cgacaccgac ctgaccgcgc caggcgcagg cttcaaggtt
     3961 tttatgcata gcataatcgc taccactaac cagaatggaa gcgtctgtaa gacggttgat
     4021 aaataaaatt gctggcaaac cctacacgaa gtcgatgctt ctgtctttag gagaagcacg
     4081 gaaagtgaaa acggttgcaa tcaggtgctt aatccatgag ccagcgtgct gaacgatacc
     4141 gggattctgt tgtcggaatg gctggttatc cattaaaata gatcggatcg atataagcac
     4201 acaaaggggg aagtgcttac taattatgaa acataaacta caaatgatga aaatgcgttg
     4261 gttgagtgct gcagtaatgt taaccctgta tacatcttca agctgggctt tcagtattga
     4321 tgatgtcgca aagcaagctc aatccttagc cgggaaaggc tatgaggcgc ccaaaagcaa
     4381 cttgccctcc gttttccgcg atatgaaata cgcggactat cagcagatcc agtttaatca
     4441 tgacaaagcg tactggaaca atctgaagac cccattcaaa ctcgagttct accatcaggg
     4501 tatgtacttc gataccccgg tcaaaataaa tgaagtgact gccaccgcag tcaaacgaat
     4561 caaatacagc ccggattatt tcactttcgg cgatgttcag catgacaaag acacggtaaa
     4621 agaccttggt tttgccggtt ttaaagtgct ttacccgatc aacagcaaag ataaaaacga
     4681 tgaaatcgtc agcatgctcg gggccagcta tttccgcgtg attggtgcag gtcaggttta
     4741 tggcctttct gcccgcggcc tggcaattga taccgccttg ccatcgggtg aagaatttcc
     4801 acgcttcaaa gagttctgga tcgagcgtcc aaaaccgact gataaacgtt taaccattta
     4861 tgcattgctt gactcgccgc gcgcgacagg tgcttacaaa ttcgtggtta tgccagggcg
     4921 tgacacggtt gtggatgtgc agtcgaaaat ctatctgcgc gataaagtcg gcaaactggg
     4981 gggtgcaccg ttaaccagta tgttcctgtt tgggccgaac caaccgtcgc ctgcaaataa
     5041 ctatcgtccg gagttgcacg actctaacgg tctctctatc catgccggta atggcgaatg
     5101 gatctggcgt ccgttgaata acccgaaaca tttagcggtt agcagcttct cgatggagaa
     5161 cccgcaaggc ttcggtctgt tgcagcgcgg tcgtgatttc tcccgctttg aagatctcga
     5221 tgatcgttac gatcttcgtc caagcgcatg ggtgactccg aaaggggagt ggggtaaagg
     5281 cagcgttgag ctggtggaaa ttccaaccaa cgatgaaacc aacgataaca tcgtcgctta
     5341 ctggacgccg gatcagctgc cggagccggg taaagagatg aactttaaat acaccatcac
     5401 cttcagccgt gatgaagaca aactgcatgc gccagataac gcatgggtgc aacaaacgcg
     5461 tcgttcaacg ggggatgtga agcagtcgaa cctgattcgc cagcctgacg gtactatcgc
     5521 ctttgtggtc gattttaccg gcgcagagat gaaaaaactg ccagaggata ccccggtcac
     5581 agcgcaaacc agcattggtg ataatggtga gatagttgaa agcacggtgc gctataaccc
     5641 ggttaccaaa ggctggcgtc tggtgatgcg tgtgaaagtg aaagatgcca agaaaaccac
     5701 tgaaatgcgt gctgcgctgg tgaatgccga tcagacgttg agtgaaacct ggagctacca
     5761 gttacctgcc aatgaataag acaactgagt acattgacgc aatgcccatc gccgcaagcg
     5821 agaaagcggc attgccgaag actgatatcc gcgccgtaca tcaggcgctg gatgccgaac
     5881 accgcacctg ggcgcgggag gatgattccc cgcaaggctc ggtaaaggcg cgtctggaac
     5941 aagcctggcc agattcactt gctgatggac agttaattaa agacgacgaa gggcgcgatc
     6001 agctgaaggc gatgccagaa gcaaaacgct cctcgatgtt tcccgacccg tggcgtacca
     6061 acccggtagg ccgtttctgg gatcgcctgc gtggacgcga tgtcacgccg cgctatctgg
     6121 ctcgtttgac caaagaagag caggagagcg agcaaaagtg gcgtaccgtc ggtaccatcc
     6181 gccgttacat tctgttgatc ctgacgctcg cgcaaactgt cgtcgcgacc tggtatatga
     6241 agaccattct tccttatcag ggttgggcgc tgattaatcc tatggatatg gttggtcagg
     6301 atttgtgggt ttcctttatg cagcttctgc cttatatgct gcaaaccggt atcctgatcc
     6361 tctttgcggt actgttctgt tgggtgtccg ccggattctg gacggcgtta atgggcttcc
     6421 tgcaactgct tattggtcgc gataaataca gtatatctgc gtcaacagtt ggcgatgaac
     6481 cattaaaccc ggagcatcgc acggcgttga tcatgcctat ctgtaacgaa gacgtgaacc
     6541 gtgtttttgc tggcctgcgt gcaacgtggg aatcagtaaa agccaccggg aatgccaaac
     6601 actttgatgt ctacattctt agtgacagtt ataacccgga tatctgcgtc gcagagcaaa
     6661 aagcctggat ggagcttatc gctgaagtcg gtggcgaagg tcagattttc tatcgccgcc
     6721 gccgtcgccg cgtgaagcgt aaaagcggta atatcgatga cttctgccgt cgctggggca
     6781 gccagtacag ctacatggtg gtgctggatg ctgactcggt aatgaccggt gattgtttgt
     6841 gcgggctggt gcgcctgatg gaagccaacc cgaacgccgg gatcattcag tcgtcgccga
     6901 aagcgtccgg tatggatacg ctgtatgcgc gctgtcagca gttcgcgacc cgcgtgtatg
     6961 ggccactgtt tacagccggt ttgcacttct ggcaacttgg cgagtcgcac tactggggac
     7021 ataacgcgat tatccgcgtg aaaccgttta tcgagcactg cgcactggct ccgctgccgg
     7081 gcgaaggttc ctttgccggt tcaatcctgt cacatgactt tgtagaagcg gcgttgatgc
     7141 gccgtgcagg ttggggggtc tggattgctt acgatctccc gggttcttat gaagaattac
     7201 cgcctaactt gcttgatgag ctaaaacgtg accgccgctg gtgccacggt aacctgatga
     7261 acttccgtct gttcctggtg aagggtatgc acccggttca ccgtgcggtg ttcctgacgg
     7321 gcgtgatgtc ttatctctcc gctccgctgt ggtttatgtt cctcgcgctc tctactgcat
     7381 tgcaggtagt acatgcgttg accgaaccgc aatacttcct gcaaccacgg cagttgttcc
     7441 cggtgtggcc gcagtggcgt cctgagctgg cgattgcact ttttgcttcg actatggtgc
     7501 tgttgttcct gccgaagtta ttgagcattt tgcttatctg gtgcaaagga acgaaagaat
     7561 acggcggctt ctggcgcgtt acattatcgt tgctactgga agtgcttttt tccgtgctgc
     7621 tggctccggt acgcatgctg ttccatacgg tcttcgttgt cagcgcgttc cttggctggg
     7681 aagtggtgtg gaattcaccg cagcgtgatg atgactccac ttcctggggt gaagcgttca
     7741 aacgccacgg ctcacagctg ctgttagggt tagtgtgggc tgttgggatg gcgtggctgg
     7801 atctgcgttt cctgttctgg ctggcaccga ttgtcttctc gttgatcctg tcaccgtttg
     7861 tttcggtgat ttccagccgt gccaccgttg gtctgcgaac caaacactgg aaactgttcc
     7921 tgatcccgga agagtattca ccgccgcagg tgctggttga taccgatcgc ttccttgaga
     7981 tgaatcgtca acgctccctt gatgatggtt ttatgcacgc ggtgtttaac ccgtcattta
     8041 acgctctggc aaccgcaatg gcgaccgcgc gtcaccgcgc cagtaaggtg ctggaaatcg
     8101 cccgtgaccg ccacgttgaa caggcgctta acgagacgcc agagaagctg aatcgcgatc
     8161 gtcgcctggt gctgctaagc gatccggtga cgatggcccg tctgcatttc cgtgtctgga
     8221 attccccgga gagatattct tcatgggtga gttattacga agggataaag ctcaatccac
     8281 tggcattgcg taaaccggat gcggcttcgc aataaaaacg tagttgcctg atgcgctacg
     8341 cttatcaggc ctacatcgtt cctgcaattt attgattttg caagattttg taggttgggt
     8401 aaggcgttca cgccgcatcc ggcaaaaaca acgagccaat aaaaataccg gcgtt
//
LOCUS       DAJVUJ010000171         8316 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.171, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000171 DAJVUJ010000000
VERSION     DAJVUJ010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8316
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.171"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(135..211)
                     /locus_tag="OQQ48_003588"
     tRNA            complement(135..211)
                     /locus_tag="OQQ48_003588"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(175..177),aa:Asp,seq:gtc)
     gene            complement(344..1075)
                     /gene="dnaQ"
                     /locus_tag="OQQ48_003589"
     CDS             complement(344..1075)
                     /gene="dnaQ"
                     /locus_tag="OQQ48_003589"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HCS1954220.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVEKKGGSCLWRA"
     gene            1140..1607
                     /gene="rnhA"
                     /locus_tag="OQQ48_003590"
     CDS             1140..1607
                     /gene="rnhA"
                     /locus_tag="OQQ48_003590"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HCS1954221.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1604..2326)
                     /locus_tag="OQQ48_003591"
     CDS             complement(1604..2326)
                     /locus_tag="OQQ48_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCS1954222.1"
                     /translation="MKPARVPQTVVAPDRWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLRETDRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            2360..3115
                     /gene="gloB"
                     /locus_tag="OQQ48_003592"
     CDS             2360..3115
                     /gene="gloB"
                     /locus_tag="OQQ48_003592"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HCS1954223.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLNKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            3187..4545
                     /gene="mltD"
                     /locus_tag="OQQ48_003593"
     CDS             3187..4545
                     /gene="mltD"
                     /locus_tag="OQQ48_003593"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HCS1954224.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRDSLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(4594..5190)
                     /locus_tag="OQQ48_003594"
                     /pseudo
     CDS             complement(4594..5190)
                     /locus_tag="OQQ48_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
     gene            complement(5221..6021)
                     /locus_tag="OQQ48_003595"
     CDS             complement(5221..6021)
                     /locus_tag="OQQ48_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCS1954225.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPLGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            6262..7176
                     /locus_tag="OQQ48_003596"
     CDS             6262..7176
                     /locus_tag="OQQ48_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1954226.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDREIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(7173..7966)
                     /gene="dkgB"
                     /locus_tag="OQQ48_003597"
                     /pseudo
     CDS             complement(7173..7966)
                     /gene="dkgB"
                     /locus_tag="OQQ48_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414743.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
     gene            complement(8128..8204)
                     /locus_tag="OQQ48_003598"
     tRNA            complement(8128..8204)
                     /locus_tag="OQQ48_003598"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8168..8170),aa:Asp,seq:gtc)
BASE COUNT         2217 a         2014 c         2014 g         2071 t
ORIGIN      
        1 tggtctacca tatgtgatta acacatttac agcataacaa aaccatagac cgaagaaata
       61 gcgggattta aatgcaaaaa aaccgcaaaa ttaaaaatct tgcggttctc tgaactcatt
      121 ttcatgagtg aatttggcgg aacggacggg actcgaaccc gcgaccccct gcgtgacagg
      181 caggtattct aaccgactga actaccgctc cgcgttgtgt tccgttggga acgaggcgaa
      241 tattacggat tgcctcaccc ttcgtcaacg gtttttctca tcttttgaat cgtttgctgc
      301 aaaaatcgcc caagccgcta tttttagcgc cttttacagg tatttatgcc cgccagaggc
      361 agcttccgcc cttcttctcc accagatcaa gacgggcttc atgagctgca agctcttcat
      421 ctgtcgcaaa aacaacgcgt aacttacttg cctgacgtac aatgcgctga attgttgctt
      481 caccttgttg ctgctgtgtc tctccttcca tcgcaaaagc catcgacgtt tgaccaccgg
      541 tcatcgccag ataaacttcc gcaaggatct gtgcatcgag taatgccccg tgtagcgttc
      601 gtttactgtt atctatttcg tagcgggcac ataacgcatc gaggctgttg cgcttaccag
      661 gaaacatttt cctcgccacc gcaaggctat cggtgacctt acagaaggta ttggtcttcg
      721 gaatatcgcg cttaagcaac gaaaactcgt agtccataaa gccgatatcg aacgctgcgt
      781 tatggatcac caactccgcg ccgcggatat agtccatgaa ctcatcggct acttcggcaa
      841 acgtgggctt atcgagcaaa aattcatcgg caataccatg tacgccaaag gcttccggat
      901 ccaccagccg atcgggtttg agataaacat ggaagttatt gcccgtcagg cgacggttca
      961 ccacttcaac ggcaccaatc tcaatgatct tgtggccttc atagtgcgca ccaatctggt
     1021 tcataccggt ggtttcggta tcgagaacga tctggcgtgt aattgcagtg ctcatagcgg
     1081 tcatttatgt cagacttatc gttttacagt tcgattcaat tacaggaagt ctaccagaga
     1141 tgcttaaaca ggtagaaatt ttcaccgatg gttcgtgtct gggcaatcca ggacctgggg
     1201 gttacggcgc tattttacgc tatcgcggac gcgagaaaac ctttagcgca ggctacaccc
     1261 gcaccaccaa caaccgtatg gagttgatgg ccgctattgt cgcgctggag gcgttaaaag
     1321 aacattgcga agtcattttg agtaccgaca gccagtatgt ccgccagggt atcacccagt
     1381 ggatccataa ctggaaaaaa cgtggctgga aaaccgcaga caaaaaacca gtaaaaaatg
     1441 tcgatctctg gcaacgtctt gatgctgccc tagggcaaca tcaaatcaaa tgggaatggg
     1501 ttaaaggcca tgccggacac ccggaaaacg aacgctgtga tgaactggct cgtgctgcgg
     1561 caatgaatcc cacactggaa gatacgggct accaagtcga agtttaagct tgtggtttac
     1621 gacattgccg ggtggctcca accgcttggc gaattcgtgg cttgttttta ctctgtttca
     1681 tcgggtttag cgttaaagga atagtccgtt tccgggcaac aataagttgt aagcaaccaa
     1741 gcgcaggaat atgcgcattc aatagttttc ctccgtgttt gttccacggg agaacgtgga
     1801 aacggctggc gtgcagcact tcaaaattca acaaagagag ccagtccagt tgccgcatca
     1861 gagtaaacat ccggctgtta tagggcgagg tttttcgcaa taccggcaca agtttgcgta
     1921 atcccattaa actaatggga ttgaagccac taatcaccag ccagccatca tcaatcaata
     1981 cccgatcggt ttcacgcaat aaacgatgcg gatcggtgca ccacggcaat gtatgtgcca
     2041 gtagacaaac atcaacggat ttatcggcaa aaggaagatg aagtggatcc gcctgcacct
     2101 ggacgggcat tccttgcgca gaaacattca cttgatgaga aaccgcgcac gcttcgcaat
     2161 tgatttctgc gcttaaattg ccaatcttaa gcagatgaaa accatacatt ttagtgaacc
     2221 acgggttgag ctggcgctcc agcgccttgc gataaagctc tccccagggc aaatcgcccc
     2281 agcgatcagg agccacgaca gtttgaggga ctcttgccgg tttcatcaca accttccgtt
     2341 tcacactaag aggtaatcta tgaatcttaa cagtattccc gcctttgatg acaattacat
     2401 ctgggttttg aatgatgaag caggtcgctg cctgattgtc gatcccggag acgcagagcc
     2461 agtattaaac gccattgccg ccaataactg gcaaccggag gccatatttc tcacccacca
     2521 tcatcacgat cacgttggcg gcgtaaaaga actggtggaa aagtttccgc aaattgtggt
     2581 gtatggtcca caagagacac aagataaggg aacaacacag gtagtcaaag atggcgaaac
     2641 tgccttcgtt ttggggcatg aatttagtgt aattgccaca ccgggtcaca ctttaggaca
     2701 tatctgttac ttcagtaaac cttatctatt ttgcggcgac acgctgtttt ctggtgggtg
     2761 tggtaggttg tttgaaggga cagcatcaca aatgtatcaa tcacttaata agttaagtgc
     2821 gttacctgac gatacattgg tatgttgtgc tcatgaatat acgttatcaa atatgaagtt
     2881 tgctttgagt attcttccgc acgatttgtc cataaatgat tattatcgta aagttaagga
     2941 gttacgggca aaaaatcaaa taacactacc cgtaattctg aaaaatgagc ggcaaattaa
     3001 tgttttttta agaacggaag atattgattt aattaatgta attaatgaag aaacattatt
     3061 gcaacaacct gaagagcgtt ttgcatggtt aaggtcaaag aaagataggt tctgataaaa
     3121 ctttcttgtc atcggctccg ttcgccgtta tgatcggtcg tcttttaagc aactattgac
     3181 acacacatga aggcaaaagc gatattactc gcctctgtcc tgctcgtggg ttgccagagt
     3241 accggcaacg ttcaacagca cgcacagagc ctttctgcag ctggtcaagg ggaagcagca
     3301 aagtttacaa gtcaggcacg atggatggac gatgggacgt ctatcgcgcc agatggtgac
     3361 ttgtgggctt tcattggcga cgagctaaag atgggaattc cggaaaatga ccggattcgc
     3421 gaacagaaac agaaatattt acgcaataag agctatctcc acgatgtaac tttacgggca
     3481 gagccgtata tgtactggat agcagggcaa gttaaaaaac gtaacatgcc tatggaactg
     3541 gtactactac ccatagtgga gagcgctttt gatcctcacg caacgtctgg cgccaatgcc
     3601 gcaggcatct ggcagatcat tccgagcacg gggcgcaatt atggtttgaa acagacccgc
     3661 aattatgacg cgcgtcgcga tgttgttgct tcaacaactg ccgcgctgaa catgatgcag
     3721 cgtctgaaca agatgtttga cggcgactgg cttctgaccg tagcggctta taacagcggc
     3781 gaaggtcggg tcatgaaggc aattaaaacg aacaaagcgc gtgggaaatc cacggacttc
     3841 tggtcgttac cgttgccgca ggaaacgaag cagtacgtgc ctaaaatgct ggcattgagt
     3901 gatattctca aaaacagcaa gcgttatggc gtacgtctgc caacgaccga tgaaagccgt
     3961 gctctggcgc gtgtgcacct gagcagcccg gttgaaatgg cgaaggttgc agatatggcg
     4021 gggatttccg tcagcaagct gaagacattc aacgctggcg taaaaggctc cacgctgggc
     4081 gcaagtggcc cgcagtacgt gatggtgcca aagaagcatg cagatcaact gcgtgattct
     4141 ctcgcttcag gcgaaattgc tgctgtacag tcgacgctgg ttgccgacaa tacgccgctt
     4201 aacagccgtg tttacaccgt acgctctggc gacacgcttt caagcatcgc ttcacgtctc
     4261 ggcgtaagca ccaaagattt gcagcagtgg aacaaactgc gcggatctaa gctgaagcca
     4321 ggccaaagtt tgacgattgg tgcaggcagt agcgcacagc ggttggcaaa caacagcgat
     4381 agcattacgt atcgtgtgcg caaaggcgat tcgctttcaa gcattgctaa acgccacggc
     4441 gtgaacatca aagatgtgat gcgctggaac agtgacactg cgaatctgca accaggcgat
     4501 aagctgacgt tgtttgtgaa aaacaacaac atgccagatt cctgacaaac cagataataa
     4561 aaaaggcacc gattcccccg gtgccttttt tatttatgcc gctttatgcg cttctaccat
     4621 gatgatatca ctggtgaaag agccatcagt ctgcaaggaa aaatacgttc tcacctctgt
     4681 cgatgcgctc tgttggtaaa tgcgaatagc gtctactaac gcttctggcg tacgcattct
     4741 cacgacccat gaagaaaatt ccagcggtaa cttatctgta attaaattat caactataag
     4801 attggcttca ttgattaacg tcaaccacgc accgctggcg tagtttcgta cgtgagaggt
     4861 atcgcgtaat gcttctaccg cctgtaacca gatgtcgcgc actgggtgac ccggagacat
     4921 tacatccatc acaatcagcc taccgccagg tttcaatatc ctattcactt ctcgcagtgc
     4981 tgcaccaaca tcatgccagt gatgggcaga ataacggctg ataacaatat caaatgcgtt
     5041 atcggcaaat ggcagacttg cggcatatcc ctggcgggtg gtgatatttt tcagttgccg
     5101 ggcttcggca gcttgtgcca cgacatccag catgtgggcc gataagtcat acgccaccac
     5161 cgcgctcacg ttttgcgcag cgccaaacgt tggtctgccc tgtggcagac ctgacatacc
     5221 ttatttatca ggcttgccgg gactgaattc aacgagtagc ggattgtgat cggaagcgcg
     5281 cgtaaccagt acagaagctt cactgacgtt cagaccacgg tagaaaacaa aatcgagcgg
     5341 gcgaccaaac gcccgacggc gctgatcatc ggtaaaacgc acctggcgca gcgacatttc
     5401 ccgcgcaaag cgatataacg cgttcatcct tctacggctc caggcattga aatctcccgc
     5461 cataatgacc gggccgctgt ggtgagctat ctgatcacca ataggaagta actgcttact
     5521 atagacatcc acgcccagac tgaagttgac ggcgtgtata ttaaccacca tcaacaggcg
     5581 ggtgtcaggt aatggataga ccgtcaccag tgccgacttc gccagacgca aaatgggttc
     5641 tcgttcacgt aacgggcagc aatacactgg atgtgccgcc gaaagggtca ttacgccaga
     5701 aggatgttgt ggcagcacga aagcgggtac ctgatcggcg gcaagatagt tagcggtcgc
     5761 aaactgtact aactctggcg ttgtctgcgc ttcctgcaat aacaccaggt gtgcatcttt
     5821 gccgtagttc tttaataccg acaaccattc agcgcgttgc tgtttgtata tgttccacac
     5881 caggatccga atacgctctt cggtacttaa cggttcccca agtggtaatg cctggccgat
     5941 gctcgcaaaa gaccccggcg gtaagatcct ttccgcaggt tgtccggcaa catagcgcat
     6001 ggcataggtg ttttttcgca cttttgactt tgaaacctct attacgtcaa cccttccgca
     6061 ctcgggaggg ttctactatt atagggattt tagctcactc tttcaacgag caattgtata
     6121 ttgttatgta agcaagtgct tacaccggtc agaattttgc tcattatgga gaacattaaa
     6181 tcgcatgaca tatcacgcat cctgatggtt taatgacaaa taaattcaaa atatctcttc
     6241 tattttgcaa caggagcaaa aatgaaagcc acgtcggaag aactcgccat ttttgtttcg
     6301 gtcgtcgaaa gcggcagctt tagccgggca gcggaacaat tagggcaagc aaactcagcg
     6361 gtaagccggg cggtgaaaaa gctggagatg aaacttggcg ttagcctgct taatcggacc
     6421 acgcgacaac ttagcctgac ggaagaaggc gagcgttatt ttcgtcgcgt acagtcaatt
     6481 ttgcaggaga tggcagcggc agaatcagaa attatggaga cgcgtaatac accgcgtgga
     6541 ctgttacgga tcgatgccgc aactccagtg gtgctgcact ttctgatgcc gttaattaag
     6601 cctttccgtg aacgctatcc ggaagtcact ttgtcgctag tctcctccga aacgattatt
     6661 aatttgatcg aaagaaaagt ggatgtcgcg atacgcgctg gtacgttaac ggattccagc
     6721 ttacgtgcca ggccgttatt taacagttat cgaaaaatta tcgcctcccc cgattatatt
     6781 tcccgctacg ggaagccaga aacgatcgac gatttaaagc aacatgtttg cctgggattc
     6841 actgaacccg cttccctcaa tacctggccg atagcctgta gtgatggaca attacatgag
     6901 gtgaaatacg gtttgtcatc caatagtggg gaaacactga aacagctttg cctgagtggc
     6961 aacgggattg cgtgtttgtc cgactacatg atcgacagag aaatcgctcg cggagaattg
     7021 gtggagttaa tggcagataa agtgttgcca gtggaaatgc cattcagtgc cgtctattac
     7081 agcgaccgtg cggtaagtac gcgcatccgg gcttttatcg atttccttag cgagcatgta
     7141 aaaacagctc ccggaggagc tgtcagagag gcttaatccc attcaggagc cagaccttcc
     7201 gggctaacca ggcggtcgtt gcaatccagt gcggcgatcg cttttttatc ttcggcatca
     7261 agctgtaaat tttgtgcctt aagattactt tccaggtttt tacgtttagt agaagaagga
     7321 attactgagt aaccttcccc catagcccac gccagaatca cttgtgccgg agtcgcattg
     7381 tgtttagctg cgatacgagc aataacctca tctttcaggg ccttaccata cgccagcgtc
     7441 atataggaag taatatggat gccgtgctgt ttagcccagg caaccacttt acggttttgc
     7501 agataaggag agagttcaat ctggttagta gcgatgtttt cagcgccaac agcagcaatc
     7561 gccttttcca tcaatgggat cgtgaagttg gaaataccga tctcacgcgt cagcccttgt
     7621 tttttggctt ccagcagcgc ctgcataacc ccttcaacag agacttcatc gtttggtgac
     7681 ggccagtgga ttagcgtcag atcaacataa tcggtacgca atttttgcag gctctctttc
     7741 agacttggga tcaatttgtc tttgctgaga ttttcaatcc agattttagt ggtgatgtag
     7801 agttcatgac gtggcacgcc actttctgca atcgcctgac ctactgcggc ttcgttatca
     7861 tagatttgtg cggtatcaat tgcgcgataa ccaagttcaa gcgccgtttt cacagatgaa
     7921 ataacaacgt agtctttcaa cctaaaccaa atgcagggat agccataata tgcctctttt
     7981 aatgctattt tcgttaactg aggtgattat gggcttcaga ctgttgagga aaaaagcgta
     8041 aaatgcagag gatttttgcg attctggcaa taatagatat acagacgaaa aaaaacctcg
     8101 agcgttaact cgaggcttct taataagtgg cggaacggac gggactcgaa cccgcgaccc
     8161 cctgcgtgac aggcaggtat tctaaccgac tgaactaccg ctccaccgaa ttcttttaca
     8221 accaccggtt ttatgaccgg cttactgctt aatttgatgc ctggcagttc cctactctcg
     8281 catggggaga ccccacacta ccatcggcgc tacggc
//
LOCUS       DAJVUJ010000172         8303 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.172, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000172 DAJVUJ010000000
VERSION     DAJVUJ010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8303
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.172"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(96..1766)
                     /gene="hscC"
                     /locus_tag="OQQ48_003599"
     CDS             complement(96..1766)
                     /gene="hscC"
                     /locus_tag="OQQ48_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HCS1954227.1"
                     /translation="MDNAELTIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEIHASAGDSFLGGEDFTHMLVDEVLKRA
                     EVARTTLNESELAALYACVEAAKCSNQSPLHIRWQYQEETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSTQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASDYLAIEIP"
     gene            complement(1850..2785)
                     /gene="rihA"
                     /locus_tag="OQQ48_003600"
     CDS             complement(1850..2785)
                     /gene="rihA"
                     /locus_tag="OQQ48_003600"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HCS1954228.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHIEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTDNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            complement(2903..3628)
                     /gene="gltL"
                     /locus_tag="OQQ48_003601"
     CDS             complement(2903..3628)
                     /gene="gltL"
                     /locus_tag="OQQ48_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HCS1954229.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            complement(3628..4302)
                     /gene="gltK"
                     /locus_tag="OQQ48_003602"
     CDS             complement(3628..4302)
                     /gene="gltK"
                     /locus_tag="OQQ48_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HCS1954230.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNIFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            complement(4302..5042)
                     /gene="gltJ"
                     /locus_tag="OQQ48_003603"
     CDS             complement(4302..5042)
                     /gene="gltJ"
                     /locus_tag="OQQ48_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HCS1954231.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAIIMLVMTLVERKVRLPGNMGGK"
     gene            complement(5212..6120)
                     /gene="gltI"
                     /locus_tag="OQQ48_003604"
     CDS             complement(5212..6120)
                     /gene="gltI"
                     /locus_tag="OQQ48_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HCS1954232.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKDKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            6575..>8303
                     /locus_tag="OQQ48_003605"
     CDS             6575..>8303
                     /locus_tag="OQQ48_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HCS1954233.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCIGIFCYLAQWMSYEEVDQSA
                     LIHLGANVAPLTLSGEPWRLLSSIFLHSSVSHLLMNMFAFLVVGGVAEQILGKWRLLI
                     TWLFSGVFGGLISACYALRDSDQIVISVGASGAIMGIAGAAIATQLASGAGTYHKNQR
                     RVFSLLGMVALTLLYGARQTGIDNACHIGGLIAGGALGWLSARLSGQNRLVTEGGIIV
                     AGSLLLTGAIWLAQQQMDESVLQVRQSLREAFYPQEIEQERRQKKQQLAEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNMLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDEKLIYATSFEANALSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVSAIDLVTYQHVADIPLEKYDGAGTSGKPGAWV
                     MALSPDERTLLVPGAGRGNIVRINTITHQKEDFPAGDARGTISAMRFRPENGEVIFAD
                     SQGISRISVGDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYV"
BASE COUNT         2072 a         2049 c         2076 g         2106 t
ORIGIN      
        1 tatgctctcg gcaaaatcgc gatgttgttt agttgacgat gtgttttttg cgccgatgcc
       61 ggatgcagcg tttacgccgc atccggcagc cgtgtttatg ggatctcaat ggctaaataa
      121 tcgctcgccc gccgccgaac ttcatccacg cgggccatgt cgtttgactg tagcgccgca
      181 tcaaaatcgg tgatcacccg gccaatctct tctcgctcgt cacccagcgc tcgcgcccac
      241 aactcttcca gtttggcttt aaaggtgcga ttgatgagca tatcgcgcgg ataaattttc
      301 aatgcggata accgcgtccg actctcttca atctgctgcg tgctcaatgt taccgggctg
      361 tggttaatca ctctggactt aacgctgccg tcttccagaa gcacgtcaac ttcaagcaac
      421 ccgttgatat cataactaaa gcgaatatca atcgactgat aagccccggt tttcttcaac
      481 ggcacatcga aggattcaac cagaatgtta tttttaactt tgtggttttc tccctgatag
      541 acgttaaccg taattgaatc ctgctccggg tgcatggtcg agtaagtttc tacacgcgac
      601 accggtactg tggtgtttcg ttcaataatc ggcgagaaaa tgccggaaac gccctggcgg
      661 ttaacttcaa cgcccaacga gtaagggcaa atatcagtga ggattacctc ttcaatatct
      721 tcactgcgta agcggcaggc ggcctggatt gctgcgccca gcgcgacaat ggtgctcgga
      781 tcgtaacttt gatacggtaa tttgccaaac agacgcacgg cgattcgctg caccagcggc
      841 atttgtgacg cgccgccaac cagcaccaga ctgtcgattt gactcggctt cagacgcgca
      901 tcgcgcaacg cctgttcaat cggcactcgc aagcgattga gcagcggcaa ccacaaatct
      961 tccagttcgt tctcgtaaaa ttcgcattcc cgcgtttctt cctgatactg ccagcgaatg
     1021 tgcaatggcg attgattgct acattttgcc gcttccacac aggcgtacaa ggctgccagt
     1081 tcactctcgt taagcgtggt cctggcaact tccgcgcgtt ttaaaacctc atcgaccagc
     1141 atatgggtaa aatcttcgcc accgaggaag ctatcgccag cggaggcgtg aatttcaatc
     1201 accggcgtgg cgtactcaag caccgtaacg tcaaacgtgc cgccaccgag atcaaaaacc
     1261 agcgagcggg tattttgttg cgtatgcagg ccatatgcca tcgcagctgc tgtgggttca
     1321 ttaattaagc gtaccgcatt taacccggct aactccgcag ctaaacgggt atgcttgcgt
     1381 tgttcatcgc tgaaataagc cggaacggag atcaccacat ctttaatcgg acgttgcaga
     1441 aattcttcgg catcttcttt taatgagcgt aataccaaag aggacagttc tggcgcgtta
     1501 aatgtgtcgc tgcctaaccg ccagttggta ttactgccca ttgcgcgttt aaataacgct
     1561 gccgttttat ccggatgcga agtacgccgt gatacagccg gttttccgac taaaatatga
     1621 ttattttcat ccatgctaat tatggatggt gttaaatatt caccgaactt atttggaatt
     1681 aattgcgcgg caccgtcttt ccagacggca attaaactat tggtagtgcc gagatcgata
     1741 ccaatggtga gttctgcatt atccattgca tttatatctt gaaagaaaaa gagataaaca
     1801 gattaagacc acccaggtaa cgcgtggtga tcttaatcaa tgacgtgtgt taagcgtaaa
     1861 atttcagacg atcagccagt aaatcaacaa agccctgacg atcaacatcg accattacag
     1921 tggcattcgg tttattgtca gtcagataat aataatcaac aaccgtcata ccctgggtat
     1981 atttcccctg tgtttccacg ccaacccagc gctcaacagt ggtaaacaac tcgggtttca
     2041 acagccaggc gatggtgcat gggtcatgca gcggtgcacc gacaaagccc catttttcgt
     2101 ctttatgata ttcgaggaag aaatccagca gttcggcaac aatggttgaa acagggttgc
     2161 caatcgcgcg gaaacgctcg gtgtcttcaa tgtggatttg cgctttatga gtaacatcca
     2221 gaccggccat caccactggg atccctgact ggaagacaat ttctgccgct tccgggtcaa
     2281 cgtaaatatt aaattcagcc gcaggcgtcc agttaccaag ccccattgcg ccgcccataa
     2341 tcacgatacg ggcaattttg ctatgcagtt ccggatggct attgagcagc aaggcaacgt
     2401 tagtttgcgg tccggtagac acaatcgtga caggttccgc actttcgcgc agcgttttcg
     2461 ccatcagctc taccgccgta cagttttgcg gtgcgaacgt cggttccggt aatgccgggc
     2521 catccagacc gctttcgccg tgcacattgt ccgcgataat caactcacgc attaacggtt
     2581 ttaccgcgcc gcctgctacc ggaatatcgg tgcgattaag caaggtcagc atacgcagaa
     2641 cattgcgtaa ggttttttct ggtgtctggt ttccggcgga agacgtaatt gctttgacat
     2701 caagctctgg tgaggcgagg gcgagaacta ttgcgatagc gtcgtcatga cctgggtcgc
     2761 aatctaacag aattggcagt gccattgttg ctccttgttg tgtgcttctt tgcgacaagg
     2821 gtaacgccag gatgtaacag atacgagggg cgaaacgata aagcgtgaga tggcgcgcac
     2881 ttgggtatgc gcgccagagt gattaatgca ggattttcgc gaggaagtct ttcgcgcggt
     2941 ccgatttcgg atcatcgaag aaagcgtctt tcggcgagtc ttcgacaatt ttaccctcgt
     3001 ccataaagat cacccgattc gccactttgc gggcaaagcc catttcgtgg gtcaccacca
     3061 tcatggtcat tccttcgttc gccagttcca ccatcacgtc cagcacttcg ttgatcatct
     3121 ccggatccag cgccgatgtc ggttcgtcga acagcatcgc aataggatcc atacacaacg
     3181 cgcgagcgat tgccacacgc tgctgctgac cgccggaaag ctgcgccgga aatttattgg
     3241 catgtgcgga aagtcccacg cgctccagca atttcaatgc tttttcacga gcgggcgctt
     3301 tatcgcgttt aagcactttc acctgcgcca gagtcaggtt ttcaataatc gacaaatggg
     3361 ggaacaactc aaaatgctgg aacaccatcc cgacgcggga acgcagcttt gccagatcgg
     3421 ttttcttgtc attaaccacg ataccatcga cggtgatttc accttgctgc actggttcga
     3481 ggccgttgac ggttttaatc agcgttgatt tgccggaacc agacgggccg caaaccacca
     3541 ccacttcgcc ttttttcact tcggttgagc agtcggtcag cacctgaaag tgaccatacc
     3601 attttgaaac atttttcagg gtaatcatta tgctgtcctt cttttcaagt agctgaccaa
     3661 caacgacgcg ctaagactaa taacgaaata aacaaatccg gcaaacagga tcatctcaac
     3721 ctgcgtacca tcacgctcac caatggttga ggcggtacgg aagaaatcgg ccagagataa
     3781 cacatacacc agtgaggtat cctggaacag tacgatgccc tgagtgagca gaagcggcac
     3841 catcgcgcgg aacgcctgcg gcagaataat cagtttcatc gactgccagt gagtcattcc
     3901 caacgccagc gcggcgctcg actgaccacg agaaatactt tgaataccag cacggataat
     3961 ctctgaatag taggccgctt caaacatcga aaacgccacc atcgccgaaa ttaaacggat
     4021 atcatttttt ggtgataatc ccagcacgtt ttgcagaaaa cctggcacga tcaggtaaaa
     4081 ccacagcaaa accataacta aaggaatcga gcggaatatg ttaacgtagg ctttggcaaa
     4141 ccacgctacg ggcgcaaagc tggacaagcg catcaccgcc agcatggtgc cccacaaaat
     4201 accaatcacg actgccgtga cggtgatttt cagcgtgatc accagcccgt cgagcagata
     4261 tggcagggaa gggacaatgg aactccagtc aaactcgtac attatttgcc ccccatgttg
     4321 ccaggcaggc gaactttacg ttcaaccagc gtcattacca gcatgataat cgcgttaatc
     4381 aacacatacg ccagcgtaat agcggtaaac gactcccagg cgtgggctga gtaatcaagc
     4441 aatttacccg cctgcgccgc catatccacc agaccaatag tcgaggcgat ggcggagttt
     4501 ttcaccaggt tcatcatctc ggaagtcatc ggcgggacga taacgcgata agcattaggc
     4561 agcagtacgt atcgataagc ctgcggtagc gtcaggccca tcgccagcgc ggcatttttt
     4621 tgcccacgcg gcaacgactg aatggcggcg cgtacctgtt cgcaaacacg ggcggcggta
     4681 aacagcccca ggcagagcat ggatgaaagg aaaaactgaa tattgggatc cagctcggcc
     4741 ttaaaccaca tgccgatttt ctccggcaac agctccggga tcaccagata ccaggtaaag
     4801 aattgcacaa tcaacggcac gttgcggaac agttcgacat acaacgtacc cagaccggag
     4861 agaaaacggt ttggaacggt acgtaaaatg ccgaaaaatg aaccgacgag gaaagcgata
     4921 atccaggcgc agatcgacaa agcgatcgtc acctgaaaac cactccagat ccaaccaaga
     4981 taggtggtgt tgccgaacgg ggcttgttgt aaaaaaatac cccagttcca gtctatagac
     5041 ataaatctac tccagaaaaa agagggtagc agcgttaact gctaccctcg aagattgtta
     5101 cccagcgtat tgcggttttc aggcccgatg gggaacgacc atcaggcgta tagtctgtcc
     5161 gtgctacgta acaatcgaga gggctggaat ttccgcccct ggttcttgta attagttcag
     5221 tgctttgtca ttcggttctt tgaacagtgc tttcatttcg tctgacagtt cgaaattcat
     5281 gttcaggttt ttcggcggaa ttggattttt gaaccattta tcaaaccatt tttccgcttc
     5341 accggaagtc tgcacctgag cgatagtgtc atccatcagc tttttgaact gcggatcatc
     5401 tttacgcaac atgcaaccgt aggcttcctg agactgcggc ttgccgacga tatcccagtt
     5461 gtctggtttc ttcgctttcg cacgttcacc ggccagcagg gcatcatcca tcataaaggc
     5521 aacggcacga ccgctttcca gggtgcggaa agagtcaccg tgatctttgg cgctgatgat
     5581 gcgcatattc attttttgct cttcattcag tttgttgagc aaaacttcag aggtagtgcc
     5641 ggaagtgacg actacggctt tgtctttcag gtcggcaaaa tctttgatat cgccgccctt
     5701 tttggtcagc aggcgcgtac cgaccacgaa aatagtatca gagaaagccg cctgtttttg
     5761 gcgttcgacg ttgttggtgg tagaaccaca ttcaaaatcg aaagtgccgt tttgcagcag
     5821 tggaatacgg ttttgtgagg taatcggaat cagttttacc tgcaagtccg gtttgttgag
     5881 tttctttttc actgcttcaa caatggcgtt ggagtaatcc tgcgagtaac ccaccacttt
     5941 ttgctgattg tcgtaataag agaaaggtac tgaagattca cggtgaccga cgacaatcac
     6001 accgtttttg gcgattttgt ccagcgtact gcctgctgcc ggggcggcgt catctgcctg
     6061 tgccagtcct gcggaaagcg ccagggcgag gattgctgtg gcaggtttac gtaattgcat
     6121 atccaactcc tttatcttct gcgttaagaa cgcatggata cccgttgtga gtgtttgtgt
     6181 tgttatcgtc tgcaacttta ttgtgcagtg ttgtgcctgt taggtaacat ttagtttggc
     6241 taaatgtaaa gatattgctg ttttattgtt tgtttttgcg agatgcgccg caccattccg
     6301 aagcaaaatt cttaaaccgc acctttttgg tgctaccgct gatttgtggt ggtgcaacct
     6361 ggttgcgtcg cggctgattt catcgcttac acagtaaata aagcaactta catgccaaaa
     6421 aatacttagg ggcaaattaa ttacgattgt aattttaggc tgaaaaagtc atatttcgga
     6481 cagaattatc ttttcggcat gggacgtttg ccgggatgat aactgcacac gggaaaaatg
     6541 ctttttacct gtcttttcaa ggaagaaaca ccttatgtct gcatcttcgg ttaagccgtt
     6601 aaatgttcaa ttacccgcaa taacccttat cctttttgca ctctgtattg ggatattttg
     6661 ttatctcgca caatggatga gttatgaaga ggtcgatcaa tccgcactta tccatctcgg
     6721 cgcaaacgtt gcgcctctta ctttgtcggg tgaaccctgg cgcttattga gcagtatctt
     6781 tctgcacagt agtgtttctc atctgctgat gaatatgttt gcattcctgg tcgtgggagg
     6841 cgtggcggaa cagattctgg ggaaatggcg actcctgatt acctggttat tctccggcgt
     6901 ctttggtggg ctcatcagcg cctgttatgc gttacgcgat agtgatcaga tagtcatcag
     6961 cgttggggca tccggggcaa ttatgggaat agctggcgct gcgatagcaa cacagcttgc
     7021 ttcaggtgcg ggcacatacc ataaaaacca gcggcgagta ttttctctgt tgggtatggt
     7081 ggcgctgaca ctgttgtatg gtgcccggca aacaggaata gataatgctt gtcacattgg
     7141 cggcctgatt gcgggtggcg cgttgggttg gctgagcgcg cgtttatctg ggcaaaaccg
     7201 actcgttacg gaaggcggga ttattgttgc gggcagtctt cttctgaccg gggctatctg
     7261 gcttgcgcag cagcagatgg atgagtcagt tttacaagtc aggcaaagcc tgcgtgaagc
     7321 gttttatcca caggagattg aacaagagcg acggcaaaaa aagcagcagt tagcggagga
     7381 acgcaacgcc ctcagggaaa cattatccgc tccggtaagt cgtgaacagg ccagtggtga
     7441 tttgctcgct gagattgccg atatccatga tatggcgatc agtcgggatg gtaatatgtt
     7501 gtatgccgca attgaaaaca cgaacagcat tgttgttttc gacctcggac aaaagaaaat
     7561 cctgcatacc tttacagcgc ccatagcgaa agaaaagtca gtcaaacatt gtggtggctg
     7621 taaagatcag ggcgtcagat cgctggcatt aagcccggat gaaaagttga tttatgcgac
     7681 ttcatttgaa gcgaatgcgt tatcggtcat taacgtggcg acaggggaga ttattcagtc
     7741 gattaccacc ggtgcacatc ctgacagcct tatcctctcg cgtgatggca caaaagcctg
     7801 ggtgatgaat cgcaccagta atagtgtgtc agcgattgat ctggtgactt atcagcatgt
     7861 ggcggatatc ccgctggaga aatacgacgg ggcggggacg agcggtaaac caggcgcctg
     7921 ggttatggca ctttccccgg atgaaagaac attactggtt ccgggagcag gcagaggtaa
     7981 catcgtgcgg atcaatacca tcacgcatca gaaagaagac tttcccgcag gtgatgcgcg
     8041 tggaacgata tcggcgatgc gttttcgacc tgaaaacggc gaggttattt ttgcagatag
     8101 tcaggggatt tcacgtataa gcgtagggga tcaacaagcc agcattatga cgcaatggtg
     8161 tagcaggagc gtttattccg ttgagggtat tagcccggac ggtcaatatt tagcgttggt
     8221 gtcatatggc ctgcaaggtt atgtcatcct gctcaatatt aatgccgggc agattattgg
     8281 cgtttatcct gccagctacg tta
//
LOCUS       DAJVUJ010000173         8216 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.173, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000173 DAJVUJ010000000
VERSION     DAJVUJ010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8216
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.173"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(<1..835)
                     /locus_tag="OQQ48_003606"
     CDS             complement(<1..835)
                     /locus_tag="OQQ48_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCS1954234.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQRLTSSPDYH
                     GP"
     gene            927..1013
                     /locus_tag="OQQ48_003607"
                     /pseudo
     CDS             927..1013
                     /locus_tag="OQQ48_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1314..1403
                     /locus_tag="OQQ48_003608"
                     /pseudo
     CDS             1314..1403
                     /locus_tag="OQQ48_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 family protein"
     gene            1427..5941
                     /locus_tag="OQQ48_003609"
                     /pseudo
     CDS             1427..5941
                     /locus_tag="OQQ48_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
     gene            5942..7591
                     /locus_tag="OQQ48_003610"
     CDS             5942..7591
                     /locus_tag="OQQ48_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HCS1954235.1"
                     /translation="MNWRRIVWLLALVTQPTLAEETPLQLALRGAQHDQLYQLSSSGV
                     TTVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIHTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            7596..>8216
                     /locus_tag="OQQ48_003611"
     CDS             7596..>8216
                     /locus_tag="OQQ48_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1954236.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKIQQGRLVVNGASMPQRVESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVY"
BASE COUNT         2004 a         1971 c         2310 g         1931 t
ORIGIN      
        1 gtgggccgtg gtagtccggc gaagaggtta atctttgcag ggcttgcaga gtgcgagtcg
       61 acaatggatt gttataaata ttaacatctg cataaatgtg cagattcatg atactgtctg
      121 gcaatgtgtt cagctgattg ttagggatat atagtgattg cagacgttgt ggaagttcgg
      181 gcagtctgtg tagttggttt tccccaacgt ttaaaagcct cagattttga ggaaggggcg
      241 gtagctcttg taatctgtta tcaaaagcac ttagttcctg tagttcacat ggtaattctg
      301 gtaaggcagt aagctggttg ttgccaacag aaagtttttg tagagccggg ggaagatcag
      361 gtagcgtctc caggctattg agaagggctg agagtgactg caaggaagat ggaagagaag
      421 gtaaacatgt taaaactctg ttaagtgaaa catcaagagc gaccaggtga ggcggaagag
      481 ccgggagcct gctcagtctg ttatcgctgg ctttaagtac agttaaggac ggagggagtt
      541 ctggcagaga gcgtagctca ttgctagaga tgttaagttc ttgtatgtgc gggggcaggt
      601 atgggagaga gcgtaatctg agtaaactta aattgagggc tggctcttga aaagccagac
      661 atattttcag taatcgaact gcctgtgttc ggtcttctgt tgcagcacct tccttggccc
      721 agttatccca gatgcggtga taatccgtga tattttgctc ttctacggat aaacgggata
      781 tacatcctaa accacaaaag gagcggaaaa aagaaggatt gtgggcaggt ttcatttatt
      841 attgatcctc attgtttata tattaatatg ttcgtgcaaa tggagtgagg attctttaaa
      901 aaaacgctcc tgtaaatagt taagaacagg aagaactgtt ggggatgtgg ctggccgaaa
      961 atgaaggggt gaagttctgg ctcaatgtgc tgactgaact gaaaaaacgc ggttggtcgc
     1021 cctacctttt catcaagcca gtagtctgcc caccactgca tcatttctct gcgcttatcg
     1081 agatactgag catggttgta aattcaacta acccagtgcg ttgacaaaaa attagcgcaa
     1141 gaagacaaaa aatcaccttg cgctaatgct ctgtatcagg tcactaatac cagctaagta
     1201 gttgattcat agtgactgta tatgttgtgc tttgtcgcat tttgtagtct attatttaat
     1261 ctgtaacaac gataatacaa tgaattttaa ttggtttttg tgattttgtc cttcttctga
     1321 catggaagga cacccgatgg atacccaacg attccaatcc caatttcatt ggcatttatc
     1381 gtttaaattt tctggcgcga tagcagcgtg cttgtctctg tctcttgtgg gaacagggct
     1441 tgctaatgct gatgattcgc ttccttccag taactatgcg ccgcccgccg ggggaacatt
     1501 ctttttgctt gctgacagca gttttagcag cagtgaagag gcgaaagtgc gactggaagc
     1561 gccggggcgt gattatcggc gctatcagat ggaagagtac ggcggcgtgg acgttcgcct
     1621 gtatcgtatt cctgacccga tggcattttt gcgccagcag aaaaacctgc atcgcattgt
     1681 ggtgcaaccg caatatctgg gcgacgggct gaacaatacg ctgacctggc tgtgggataa
     1741 ctggtacggc aaatctcgcc gcgtaatgca gcgtactttc tcttctcagt cacggcagaa
     1801 tgtgactcag gcattacctg aattacatct cggcaatgcc attattaaac cttcccgtta
     1861 tgtacagaac aaccagtttt ccccgctgaa aaaatatccc ttggtggaac agttccgtta
     1921 tccactatgg caggctaaac cggtcgagcc gcagcaaggg gtaaaactgg aaggcgcatc
     1981 cagcaatttc atctcgccgc agccgggtaa catttatatt cctctcggcc aacaagagcc
     2041 gggactgtac ctcgtcgagg cgatggttgg tgggtatcgg gcgacgacgg tggtgtttgt
     2101 ttccgatacc gtggctctta gcaaagtgtc aggcaacgag cttctggtgt ggaccgcggg
     2161 taaaaaacag ggtgaagcga agcccggctc agagatcttg tggactgacg gtcttggcgt
     2221 gatgacccgc ggtgtgaccg atgacagcgg taccttgcag ttacaacata tatcgccaga
     2281 acgttcatac attctgggta aggatgctga aggcggcgtt tttgtctccg agaacttctt
     2341 ctacgaaagc gaaatctaca acacccgctt gtatattttt accgatcgcc cgctatatcg
     2401 cgcaggcgat cgtgtcgatg ttaaagtgat gggccgcgag ttccacgatc cgttgcattc
     2461 atcccccatc gtcagcgccc cggcgaagct ttcggtgctg gacgctaacg gcagtctgtt
     2521 gcaaaccgtc gatgtcacgc tggatgcgcg caatggcggg cagggaagtt tccgcctgcc
     2581 agaaaatgcc gttgccggag gttatgagtt acgtcttgct taccgcaatc aggtctatag
     2641 cagcagtttt cgcgtggcaa actacatcaa gccacatttc gagattggtt tagctctcga
     2701 caaaaaagag ttcaaaactg gcgaagcggt cagcggcaaa ctgcaactgc tctacccgga
     2761 tggcgagccg gtaaaaaatg cccgcgtgca gttaagtttg cgcgctcagc aattatcaat
     2821 ggtcggtaac gatttgcgtt atgccggacg tttccccgtg tcgctggaag gcagcgaaac
     2881 ggtgtccgac gccagcggtc atgtggcgtt aaatctcccc gccgccgata aaccgagccg
     2941 ttatttgtta accgtctccg ccagtgacgg cgcggcgtat cgtgtcacca ccaccaaaga
     3001 gatcctcatt gaacgcggtc tggcgcatta ctcattaagt actgccgcac aatacagtaa
     3061 tagcggcgag tcggttgtgt tccgttatgc cgcgctggaa tcttcaaaac aggttcctgt
     3121 tacgtatgaa tggttgcgtc tcgaagaccg cacgagccat agcggagagc taccgtcagg
     3181 cggcaaatcc tttaccgtca atttcgctaa acctggcaac tacaatctga cattacgcga
     3241 taaagacggc ttaattctcg ctgggttaag ttatgccgtc agcggtaagg gcagcacggc
     3301 gcatacgggt acggtagata tcgtggcgga taaaacgctg taccagccag gcgaaaccgc
     3361 gaagatgctg attacctttc cggagccaat tgatgaagca ttattgacgc tggaacgcga
     3421 tcgcgtggaa cagcagtcgc tgctttcgca tccggcaaac tggctaacgc tacaacgttt
     3481 aaacgatacc cagtatgaag cccgggttcc agtgagcaat tcctttgcgc ctaacatcac
     3541 tttttcggtg ctgtataccc gtaatggtca gtacagtttt cagaacgccg ggatcaaagt
     3601 tgccgttcct cagcttgata tccgggtgaa aacggacaaa acccattacc agccaggtga
     3661 actggtcaat gtcgaattaa cctcgtcgct gaaaggtaaa cctgtttctg cgcagctaac
     3721 ggtaggcgtg gtcgatgaaa tgatctacgc gctgcaacca gaaatcgcgc cgaatatcgg
     3781 caaatttttc tatccgctgg ggcgtaacaa tgtgcgtacc agctccagtc tgtcgtttat
     3841 cagctacgac caggcgctct ccagcgagcc ggttgcgcct ggcgcaacta accgcagcga
     3901 gcggcgagta aaaatgcttg aacgtccacg gcgtgaagag gtggataccg cggcatggat
     3961 gccgtcactc acaaccgata aacaaggcaa agcgtatttc acgttcctga tgcctgattc
     4021 gttaacccgc tggcgtatca ccgcgcgtgg gatgaacggc gacgggctgg tcgggcaggg
     4081 gcgtgcttat ctgcgttcgg aaaaaaatct ctacatgaag tggagtatgc caacggtgta
     4141 tcgcgtgggc gacaaaccgg cggcaggact gtttatcttc agtcagcagg ataacgaacc
     4201 ggtggcgctg gtgactaaat ttgcaggcgc tgagatgcgc cagacgctga cgctgcacaa
     4261 aggggcgaat tatatttcgc tggcgcagaa tattcagcaa tctggcttgt taagtgcaga
     4321 actgcaacaa aatgggcaag tgcaggacag cattagcaca aaactgtctt ttgtggacaa
     4381 cagcaggccc gttgaacagc agaaaaatgt catgctcggc ggtggcgaga acgcgctgat
     4441 gttgcccgag caggcgagta atatccggct acaaagtagt gaaacgccgc aggagatttt
     4501 ccgcaacaat cttgatgcgt tagtcgatga accgtggggt ggggtgatca acaccggtag
     4561 ccgtctgatc ccgctcagtc tcgcctggcg ttcgcttgcc gatcatcaaa gtgccgccgc
     4621 taacgacatt cgtcagatga ttcaggataa ccgtctgcgg ctgatgcaac tggcggggcc
     4681 cggagcgcgc tttacctggt ggggtgaaga tggcaatggt gacgccttcc ttacggcatg
     4741 ggcatggtac gccgactggc aggccagcta ggcgctcggc gtaacgcaac aaccggaata
     4801 ctggcagcat atgctcgaca gttatgccga gcaggcggat aacatgccgt tattgcatcg
     4861 ggcgctggtg ctggcgtggg cacaggagat gaatctgccg tgcaaaacgt tgttgaaagg
     4921 gttggatgaa gctatcgccc ggcgcggaac taaagatgaa gatttctctg aggaagacat
     4981 ccgcgatatc aatgacagcc tgatcctcga tacaccggaa tctccactgg cagatgcggt
     5041 ggcaaacgtc ttaaccatga cgttgctgaa aaaagcgcag ttgaagtcca cggtgatgcc
     5101 acaggttcag caatatgcgt gggataaagc ggcaaacagc aatcagccgc tggcgcacac
     5161 ggttgtgctg ctcaatagcg gtggtgacgc tacccagacg gccgctattt taagtggttt
     5221 gaccgctgag caatccacta ttgagcgcgc gctggccatg aactggctgg cgaaatatat
     5281 ggcgacaatg cctccagttg ttttgcctgc gcctgcgggc gcatgggcta aacataagtt
     5341 aactggaggg ggcgaatact ggcgttgggt tggtcagggc gtgccggaca ttctctcttt
     5401 tggtgacgaa ttatcgccgc aaaatgtgca ggtccgctgg cgtgagccgg caaaaatggc
     5461 tcaacaaagt aacattccgg tgaccgttga acgccagttg tatcggctta tccctggtga
     5521 agaagagatg agctttattc tgcaaccggt gaccagcaat gagattgaca gcgatgcgct
     5581 gtatctcgat gacatcacgc ttaccagcga gcaggatgca gttctgcgct acggtcaggt
     5641 ggaagtaccg ctgccaccgg gagccgacgt tgagcgcaca acatggggca tttcggtcaa
     5701 taaacccaac gccgcgaaac agcaggggca attgctggaa aaagcgcgaa atgaaatggg
     5761 cgaactggcc tatatggtgc cggtgaaaga actgacggga acggtcactt tccgccattt
     5821 gctgcgcttc tcgcaaaaag ggcaattcgt tctgcctcct gctcgttatg tgcgttccta
     5881 tgcacctgca cagcaaagtg ttgcggcagg gagtgaatgg accgggatgc aggtgaaata
     5941 agtgaactgg cgcagaatcg tctggctatt ggcgttggtt actcagccaa cgcttgcgga
     6001 ggagacgccg ttacaactgg cactgcgtgg tgcgcagcac gatcaactgt atcagctgtc
     6061 gtcatcaggc gtgaccacag tctcagcatt acctgactcg ctcaccacgc cgttagggag
     6121 tttgtggaaa ctgtatgttt acgcctggct ggaggatacc catcaaccgg agcagcccta
     6181 ccagtgtcgc ggaaactccc cggaagaggt ttattgttgt caggctgggg agagcataac
     6241 gcgcgatacc gcgctggtac gttcctgtgg gctctatttt gcgccgcagc ggttgcatat
     6301 tggcgcagat gtgtgggggc aatactggca acagcgtcag gcgcctgcgt ggttagcgtc
     6361 tcttactacg ctgaagccag aaacctcggt aacggttaaa tcgctgcttg attcattagc
     6421 gacgttacct gcgcaaaaca aagctcagga agtcttactg gatgtggtgc tggacgaggc
     6481 gaaaatcggt gtcgcatcca tgctcggtag tcgggtacgg gtgaaaacct ggagctggtt
     6541 tgccgatgat aaacaggaaa tccgtcaggg tggttttgcc ggctggttaa ccgatggtac
     6601 accgctgtgg gttactggca gtggcaccag caaaacggta ctaacccgtt atgcgacagt
     6661 gcttaatcgg gttttgccag tgccgacgca ggttgccagt gggcaatgtg tagaggtgga
     6721 gctgtttgcc cgctatccgc tgaagaaaat cacagcggaa aagagtacga cggcagtaaa
     6781 acccggcgta cttaatgggc gctatcgtgt gacgtttact aatggtaacc acattacgtt
     6841 tgttagccac ggtgagacga ccttactgag cgaaaagggc aaactgaaat tgcagtcgca
     6901 tctcgatcgt gaagagtacg ttgccagagt tctggatcgc gaggcgaaaa gtacgccccc
     6961 tgaggcagcg aaagccatga ccgtggcgat tcacactttt ttacagcaaa acgccaatcg
     7021 ggaaggcgat tgcctgacca tccccgacag cagcgccacg cagcgtgttt ctgcttcgcc
     7081 agcgaccacg ggggcgcgaa cgatgaccgc ctggacgcag gatctcatct atgcaggcga
     7141 tccggttcat tatcatggca gtcgagctac cgaagggaca ctttcctggc ggcaagccac
     7201 ggcccaggcg gggcagggag agcgttacga ccagatactc gcgtttgctt atcccgacaa
     7261 cagccttagc cgctggggag cgccacgctc aacctgccag ttattaccca aagcgaaagc
     7321 ctggctggcg aaaaaaatgc cgcagtggcg gcgtatatta caaggcgaga cggggtacaa
     7381 cgaaccagac gtgtttgcgg tctgtcgttt agtctcaggt ttcccctata ccgatcggca
     7441 gcagaaacgg ctgtttattc gtaacttctt tacgcttcag gatcggctgg atttaaccca
     7501 tgagtatctg cacctggcct ttgatggtta tcccaccggg cttgatgaga actatatcga
     7561 aacgctgacc cgacaattat taatggacta atgctatgcg aaagattttt cttccgttgt
     7621 tactggtggc actttcgcct gtcgctcaca gtgaaggtgt gcaggaagtc gagattgacg
     7681 caccgctctc tggctggcat ccggcagagg gcgaagatgc cagtttttca caatcgatca
     7741 attacccggc gtcgtccgtc aacatggcag atgatcaaaa tatctcagcg cagatccgcg
     7801 gcaaaataaa gaattatgct gcggcgggta aaattcagca gggccggctg gtggtcaacg
     7861 gtgccagtat gccgcagaga gttgaatccg atggttcatt tgcacgccct tatattttca
     7921 ctgaaggcag caacagcgtg caggtcatca gcccggatgg gcaaagccga caaaaaatgc
     7981 agttttactc aacgccgggt acaggaacga ttcgtgcacg tttacggctg gttctctcgt
     8041 gggatacgga caataccgat ctcgatctcc atgttgtcac gcccgatggc gaacacgcct
     8101 ggtacggtaa caccgtgctg aaaaacagtg gtgcactgga tatggatgtc acgacggggt
     8161 acggacccga gattttcgcc atgccagcgc cagttcacgg acgctatcag gtgtat
//
LOCUS       DAJVUJ010000174         8148 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.174, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000174 DAJVUJ010000000
VERSION     DAJVUJ010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8148
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.174"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..260
                     /locus_tag="OQQ48_003612"
     CDS             <1..260
                     /locus_tag="OQQ48_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HCS1954237.1"
                     /translation="VVLGMIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRF
                     YRLNGDTLRRIQIHLLDKYRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(399..1028)
                     /locus_tag="OQQ48_003613"
     CDS             complement(399..1028)
                     /locus_tag="OQQ48_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HCS1954238.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATARRYPLTPLLYTLIFLHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITG"
     gene            complement(1151..2797)
                     /gene="fumB"
                     /locus_tag="OQQ48_003614"
     CDS             complement(1151..2797)
                     /gene="fumB"
                     /locus_tag="OQQ48_003614"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCS1954239.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGIAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDTCHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(2875..4215)
                     /gene="dcuB"
                     /locus_tag="OQQ48_003615"
     CDS             complement(2875..4215)
                     /gene="dcuB"
                     /locus_tag="OQQ48_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HCS1954240.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(4786..5505)
                     /gene="dcuR"
                     /locus_tag="OQQ48_003616"
     CDS             complement(4786..5505)
                     /gene="dcuR"
                     /locus_tag="OQQ48_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HCS1954241.1"
                     /translation="MSNVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(5502..7133)
                     /gene="dcuS"
                     /locus_tag="OQQ48_003617"
     CDS             complement(5502..7133)
                     /gene="dcuS"
                     /locus_tag="OQQ48_003617"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HCS1954242.1"
                     /translation="MRHSLPYRMLLKRPMKLSTTVILMVSAVQFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVVSGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYSLKTANNYKEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESLLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            7314..7544
                     /locus_tag="OQQ48_003618"
     CDS             7314..7544
                     /locus_tag="OQQ48_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HCS1954243.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVVTVVKVIDTCPSGALKYRHK"
     gene            7556..7828
                     /locus_tag="OQQ48_003619"
     CDS             7556..7828
                     /locus_tag="OQQ48_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCS1954244.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            8055..>8148
                     /gene="ghoS"
                     /locus_tag="OQQ48_003620"
     CDS             8055..>8148
                     /gene="ghoS"
                     /locus_tag="OQQ48_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HCS1954245.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLM"
BASE COUNT         2021 a         1969 c         2085 g         2073 t
ORIGIN      
        1 cggttgtgtt agggatgatt ggctatgtac cgaatgttga acagtctacg caagccctat
       61 taggtatgca gtttattatg attgctctac caactctgtt tttcatggta acgctgattc
      121 tctacttccg tttctatcgc ctcaatggtg acacgctgcg caggatccag atccatctgc
      181 tggataaata tcgcaaagta ccgcccgagc ctgttcatgc tgatattccg gtcggtgcag
      241 tgagtgatgt gaaagcctga cgtggacaat agctaacgaa aatggcctga tgcgatatgt
      301 ttatcaggcc acgtcttaat gtaatgcttt ggatttgcaa attttgtaga ccggataagg
      361 aattcacgct gcatccggca tcaacaaagc gcacgttgtt atccggttat caagccaaag
      421 cgccgtagct ggcggcaatg aaagcgagcg aggaatatca ccgtcgttaa tgcgccaagc
      481 agcgcgcaga acatatcgga ttgcgtgtcc cactggtcgc cctgggtgcc gagaaaatca
      541 tccgctccct gacccattgc cagcgccgcc caccactcga ttaattcata cattgcgctt
      601 atcgccagcg ccacgcagca gactaggaac gccaccattt tacgtccgcg cacgtacatc
      661 ccgcgaacga gaatttctcg tgccaccagt gcaggcacca gcccctggaa aaagtgcccc
      721 agcttgtcat acggattacg gctcaacccc agccattcct gcacctcaaa accaacgggg
      781 actttcgcgt aggtgtattg tccgccgacc atcaggatga tggcgtgaag gaaaatgagc
      841 gtatagagca gcggcgttaa cggataacgt ctggcggtgg caagcagtag cggcacgaca
      901 ataatcaccg gtgtcacttc catcagccag gtgagtttgt catgggccga aatgccggta
      961 taaatcagga ttaatgttag cgccagtgcg ccggtgttaa gaattaacgg cttgagtgtg
     1021 cgggtcatgg tgagttcgca aatcagggaa aatactgact attcaccagc aagcgtgaaa
     1081 ttacaatcgc aaaagaggtg gcccaggggg atcacctggc agcatgctgc caggcgctgg
     1141 gccgaagagg ttacttagtg cagttcgcgc actgtttgtt gacgatttgc tggaagaagt
     1201 cgttaccttt gtcatcgacc aagataaacg ccgggaaatc ttctacttcg attttccaga
     1261 tagcttccat acccagctcc ggataagcga cgcattccag atgcttgatg ctctgctgcg
     1321 ccagtaccgc cgccggaccg ccgatgctac cgaggtagaa gccgccgtgt ttatgacacg
     1381 tgtcggtaac ctgctgactg cggttacctt tcgccagcat gatcatgctg ccgccgtggg
     1441 attgcagcag atccacgtag gagtccatac ggcctgcggt ggttgggcca agtgaacctg
     1501 atggataacc ggcaggggtt ttcgccggac ccgcgtagta gatcgggtga tctttgatgt
     1561 actgcggaag ttctttaccg gcgtcaatca gctctttcag cttggcgtgt gcaatatctc
     1621 ggcccacgat aatggtgccg gtgagcgaca aacgagtgga taccgggtat tgcgaaagct
     1681 gggcgaggat ctctttcatc gggcggttaa ggtcaacttt caccgcttcg ccttcaccgg
     1741 cctggcgcag ttcttgtgga atgtactggc ctgggttgtg ttccagtttt tcgatccaga
     1801 taccttcgcg gttgattttc gctttaatgt tacggtcagc ggagcaggag acgcccatgc
     1861 cgaccgggca ggatgcgccg tgacgtggca gacggataac gcgaatgtcg tgcgcgaagt
     1921 atttaccgcc aaactgtgcg ccaagaccga gtttctgggc ctcttccagc agttcctgtt
     1981 ccagctggac atcgcggaac gcctgaccat gttcgttccc ttccgtcggc agttcatcgt
     2041 aatagtgagc gcttgctaac ttgacggttt tcaggttggt ttccgcagac gtaccgccaa
     2101 tcacaaacgc gatatggtac ggcgggcagg ctgcagtacc gagggtacgc attttctcga
     2161 cgaggaagtt tttcagtttg ccgggagtca gcagggcttt ggtttcctgg tagagatacg
     2221 ttttgttggc agagccgccg cctttcgcaa cgcaaaggaa tttgtactca tcgccatcta
     2281 ccgcgtacag gtcgatttgc gcaggcaggt tagtgccggt gttgacctct ttgtacatgt
     2341 ccagcgccgc attctgtgaa tagcgcaggt tatcttcgat ataggtgtta tagacgcctt
     2401 tcgacagcgc ttcttcatca ccgccgccgg tccacacgcg ctggcctttt ttaccgacga
     2461 tgatcgcgat gccggtatcc tggcaggtcg gcagcacgcc tttggcggcg atttcggaat
     2521 ttcttaagaa ttgcagcgcc acgtacttgt cgttttcgct ggcttctgga tcgtgaagaa
     2581 tagccgcaac ctgtttctgg tgtgccgggc ggagcataaa agaagcgtcg tgaaaggctt
     2641 gctgcgccag cagggtcagg gcttctggtt ccactttcag gatggtttcg ccgtcgaagt
     2701 cggcaacgct aacgtaatcg gaagtgagta gatagtattc ggtattgtct ttccccatcg
     2761 ggaaaggtgc ctggtagata aagggtttgt ttgacatagc ttccagcctg taactctgta
     2821 tttgttattc gaaaatggcg tgccgcgtag tcacgggcgg cacgcaaagt gcatttataa
     2881 gaacccgtac atcgcggcga agatccagcc gaagacgcac gatacgctca caccaatcaa
     2941 ccccggcaga ataaagctgt ggttgatgac gaagcgaccg atgtgggtgg tgccggaacg
     3001 gtcaaactga atcgctgcca gatcgctcgg ataagtcggc aggatgtaat aaccgtagca
     3061 agccggtgct gaagccacga tgtatgccgg atcaacgccg atcgccaacg cgaccggaac
     3121 aatcgccgcc agcgccgcag cctgagagtt tacaaacttg gaaaccagca gcagaacaat
     3181 ggcataggcc cacggatact ctttcaccat ttcacccagt acgccctgaa tttcagacat
     3241 atgcgcaccg aacatggttt ctgccatcca tgcgataccg tacaccgcca cgatggcgat
     3301 cataccggaa cggaagactt cgttttttga gatagacgcg ggattggttt tggtcaggat
     3361 aataatcagc gccccggtca gcagcataaa catctgaata accagtacca tcgacagcgg
     3421 tttgccgccg aaggatggac gcaggtccga atcagcacca agaagggcga ctacagcgat
     3481 tgccccgagg aaaatccaca ttgccagcca gttgcttttc ggcagttttt tatccagcag
     3541 cgtcgcggta tcaccgtaaa catactcacg gttttccggt acggagatga atttctggaa
     3601 ctcttcgtct ttatccagat ctttaccacg gaaccagctg aagataccga tcgccaggat
     3661 accgattaac gtcgatggaa tggtgattgc cagcaggtcg aggaactcaa gatggcgacc
     3721 atcaaaggtg acattaccca gcatcgcaac cagagacacg accgcaaccg acaccggact
     3781 ggcgataatc cccatctgtg caccgataga acttgccgcc atcggacgtt ccggacggat
     3841 gttgttctta atggcgacgt cgtagatgat cggcagaatg gtgtaaacca catgacccgt
     3901 accgcaaaga atggtcagcg tacaggtcac aaacggcgcg acaattgaga catatttcgg
     3961 gttgcggcgc agcaacttct cggcaatttg cagcatgaca tcaagaccgc ccgaagcttg
     4021 caaggtcgcc gatgccgcca ccaccgcaat gataaccagc atgacatcaa ccggtggttt
     4081 acctggctga aggtggaaga cgaagaccag aatgaccaga ccgataccgc ctaataaacc
     4141 cagcgcgata ccaccctttc tggcaccata aaacagacat atcagtatta tgataagttg
     4201 gatagtaaat aacatgtgtg aaccctcgcg ataatcctat ttaaattttt gctgaataga
     4261 tcacagtcac gttctgtttt gtatgaactg ttttcagagc ctgactaaat atccgtttgg
     4321 tcgttaccgg ctttagcaaa tacctcacag taaatattgg ctgattaaag cagcggcaaa
     4381 tttatgcact gtgataagcg gctttttcag agagaaaaac tctctggcga tgggtttata
     4441 gtttgtactg cgcagatgtt aatgttttgt ttgctgttat cttattgata tttatcggtc
     4501 tattttttgt tctcagggca gatatcatta ggtttaaaga ttaatcttat ctacccattt
     4561 gtgggtgaaa atacacataa tgcgggtgac ataatagtta attaactttt gttagcgttt
     4621 tgaaattaaa aacaccgttc acctgaagag atattaattt ttagcgatga tggagggata
     4681 attatatttg atctggcaca agttttactg atgaaggatg taacttgtgc caggggtatt
     4741 ttgcattacg gtaataatta ttacttacag ataaccagcg atccgttatt ggcaatattg
     4801 tttcagtagt gagtagtgtt ctgcctgaat acggtaacgg taaaccggac gccccgtgac
     4861 gccataatgg atactggtga acaagatgtg gcagttgacc agccagatga ggtatttacg
     4921 gcaggaaaca cgcgagatgt taacctcgtt agccagctcg tcggttgaaa actcatagtc
     4981 ctgatgcgcg tcaatccact ggcacagcgt gcgtaacgtc tgcggcgtta agccttttgg
     5041 caagcgacga ggatcctgtt cgttggagct gctgccgtgg attagctgat caagctcggc
     5101 ctggtcataa tactgatgtt tttccagcgc cattttcttt tgccgccagc cggtgagcgc
     5161 ctcttcaaag cgggaagcct ggaagggttt gatcaggtaa tccacgacac cgtaatgcag
     5221 cgaatcttta atggttgccg catcggctgc ggaggagatg acaatcacat cacttttgca
     5281 acgcgcgtta tgcaggacag gcagtaaatc gagcccgttc tctttttgca tatagatatc
     5341 gagcaatatc aggtcgatag gcgtatcgct attgaagata atctctttgg ctttctccag
     5401 cgtcgaggct gttccacagc attgaaagcc tgggatttgt gctacgtatc ggcgattcag
     5461 ctccgcgacc attgcgtcgt catcgataat taatacattg ctcatctgtt tgacctctcc
     5521 ccgtcccagg gtatctggac aaaaaattgt gtgaaaatcc cgggttccga ttccacggcg
     5581 atgctgccgc cgagattttc tacctgttgt ttgacaagtg ctaaaccgac gcctcgctcg
     5641 cttccttttg tcgagacacc tttgtcaaaa atgtgatcga ttttgtcggg tgcgatcccc
     5701 ggtccatcat cgttaacttc gcagtgcagc cagccgtgac ggtagtgcaa tgttacgcta
     5761 atttcgccac cgggttccgg ccctaatgct tccagcgcgt tttctatcag atttcccaat
     5821 gtggtaatca gcgtcgcgac ctggtcctca ctgccgctgt ctggcagcag gctttcactg
     5881 tttaaaatca gcgtatggcc taaatcggtc gcgcggttaa tcttgctgat taaaaaacca
     5941 gcgataaccg gagatttgat cttacccagc agagagccaa tctcttcctt atagttattg
     6001 gctgttttga gactgtaatc ttccaactgc ttataactct tcagatgcaa taatccgaga
     6061 atcacatgca atttattcat aaattcgtgg gatcgttcac gaagtgcatc agcatagttg
     6121 accagaccgt cgagtcgctg catcagttta cgaacttcag ttttgtccct gaaggttgaa
     6181 atggcaccga tgataacgcc attactgcgc accggaacgg tgttgatcag taatagccgg
     6241 tctttaatcg taatctcttc gtcgcggcgc ggcgtaccgt cgcgtaacac ttccgagaca
     6301 tctaccacct gtgaccatga gtggcttagc gtcgacagtt tctcatcgtc ctgcgactta
     6361 cggtaattca gcaattcttg tgcggcatcg ttgatcagcg tgacctcgcc gcgatcgtcc
     6421 acggcaacga cgccttcttt gatagactgc aacatggcct ggcgctgctc aaacagcgtg
     6481 gagatttcgt agggttccag gccgaaaagg atttttttca gtaccttaac cagaatgcag
     6541 gtgccaatca gtccgaccag catgccaaat aataccgacc agataatgct ccagcgactg
     6601 tcattgattt gttgggtcac gcggcttaac tcaaggccgc tcaccaccac gccaatttgt
     6661 ttatgatttt catcgtagat gggggtaaat acgcgtaaag cctgcgccag aaaaccgcga
     6721 ttgatagcga cattttcttt gccattcagc gctttaagga tgtcatcacc tttaaatggc
     6781 tgaccaatac gctgggcttc aggatgcgag tagcgaagac tttgcatatc cgtaacgaca
     6841 ataaacagca gatcgttgcg tttgcgcacg gcttccgcga tggcctggat tccgctctcc
     6901 tgcggttttt tctgcaagcc ctgacggatt tccggcgagt cggcgagggt acgcgccact
     6961 gccagtgcct tgttggccag cccgtctcgt gtcatatcac tgatttgcga gaagtaaatc
     7021 agatgcacca ccaatagcac cgagaactgt accgcgctga ccattaagat cactgtggta
     7081 ctcaatttca tcggacgttt gagtaacatg cggtagggca atgaatgtct catcagcttc
     7141 cttgtgtgac aaatttctta agcattatct ctgatgaggc gggtaattca aagggcgtaa
     7201 gaatgattgg ctatataggg gaagagactc tggcaacgga aactgccagt gctgtatgaa
     7261 gattccaggg ctatgcttat agcgataatc atactgatga gagagggaaa gtcatggatc
     7321 aggcgctact ggacgagggt tatcgctgtt ataccggcga aaagatcgat gtctatttca
     7381 acactgcgat atgtcagcat tctggcaatt gcgtacgtgg caacggcaag ttatttaatc
     7441 tcaaacgaaa gccgtggatc atgccggatg aagtcgacgt cgtcactgtg gttaaagtga
     7501 ttgatacgtg cccgagcggc gcgctgaaat accgtcataa ataagcgagg gtaaaatgga
     7561 aatacgcgaa ggccacaata aattttacat taatgacgaa cagggcaagc aaatcgctga
     7621 aattgtcttt gtgccgaccg gagagaattt agcgattatc gaacataccg atgtcgatga
     7681 aagcctgaaa gggcagggga ttggtaaaca gctggttgcg aaagtcgtgg aaaaaatgcg
     7741 tcgggaaaaa cgaaaaatta tcccactatg cccatttgcg aaacacgaat ttgataaaac
     7801 gcgggagtat gatgatattc gcagttgatg ggagagtaca gagtcacgat atttttcatg
     7861 ctctccgcgg agtgatgcag gagagcatct gaagggtagg gggatgcaca aagaatgggc
     7921 agagagcgcg tttttttgtc ccacgtcatc ccctttactg agcaaaaaaa agaatatctc
     7981 ctatatgaga atcatcaatc ggggttaata agttttgcgt ccccagagca ttcaatattg
     8041 ataggagtca tattatggaa ggtaaaaaca agttcaatac ttatgttgtt tcttttgatt
     8101 atccatcatc ttattcctca gtgttcttaa ggttaagatc attgatgt
//
LOCUS       DAJVUJ010000175         7899 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.175, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000175 DAJVUJ010000000
VERSION     DAJVUJ010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7899
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.175"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..1471
                     /locus_tag="OQQ48_003621"
     CDS             <1..1471
                     /locus_tag="OQQ48_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1954246.1"
                     /translation="VLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEGKTC
                     RQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDAFHA
                     RASHYHAGECLRQLAALNSRLNCAQEMARRDSVGEVPPVPWRTVVGSGIAGEAKLDHL
                     RLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSFQAG
                     ALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALREYL
                     RQRPPACIRPLNQVDNLFILPVAECISLGWDSGRQTLDAQVISGEGEDNLLTLSLPAS
                     ACSPFAVERMAALLQQTDDPVSLVSGFVSFVEGQLTLEPRVMMTKTRAWALDAETAPV
                     APLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLTAVGFY
                     RLAHVLVQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            1596..2057
                     /locus_tag="OQQ48_003622"
     CDS             1596..2057
                     /locus_tag="OQQ48_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HCS1954247.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASISATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(2098..2568)
                     /locus_tag="OQQ48_003623"
     CDS             complement(2098..2568)
                     /locus_tag="OQQ48_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HCS1954248.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(2615..3334)
                     /gene="yehT"
                     /locus_tag="OQQ48_003624"
     CDS             complement(2615..3334)
                     /gene="yehT"
                     /locus_tag="OQQ48_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HCS1954249.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(3331..5016)
                     /gene="btsS"
                     /locus_tag="OQQ48_003625"
     CDS             complement(3331..5016)
                     /gene="btsS"
                     /locus_tag="OQQ48_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HCS1954250.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLQLPWRDEA"
     gene            5238..5303
                     /locus_tag="OQQ48_003626"
                     /pseudo
     CDS             5238..5303
                     /locus_tag="OQQ48_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
     gene            5531..5779
                     /locus_tag="OQQ48_003627"
     CDS             5531..5779
                     /locus_tag="OQQ48_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCS1954251.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(5895..6164)
                     /locus_tag="OQQ48_003628"
     CDS             complement(5895..6164)
                     /locus_tag="OQQ48_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCS1954252.1"
                     /translation="MLLQHSDIEIAWYASIQQEPNGWKTVTTQFYIQEFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNCVDTSTAPDIEWPEEPDTM"
     gene            6221..6889
                     /locus_tag="OQQ48_003629"
     CDS             6221..6889
                     /locus_tag="OQQ48_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCS1954253.1"
                     /translation="MVKLMKKNTDDGAKIYTPITLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFETYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            7205..>7899
                     /locus_tag="OQQ48_003630"
     CDS             7205..>7899
                     /locus_tag="OQQ48_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCS1954254.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTLLQQTA"
BASE COUNT         2141 a         1935 c         1786 g         2037 t
ORIGIN      
        1 cgtactggca gtacaggcct ttgtccaggc aaaagcgcag caagcggaat tgactcattt
       61 aatctggcag atgcgcagcg aacacgtcac atcatctgac gatccgtttg ccagcgaaga
      121 aggcaaaacg tgtcgtcaat atgttcaaca actaagccag gcattatggc tgagcggcat
      181 cagccagccg cttattcact acgaggccgc tttcagccgc gcgcagcaag cagcggaacg
      241 ctgcagctgg cgatgggtga gtgaatcgct acggcaactg cgcgccagcg ttgatgcctt
      301 ccacgcccgc gccagccact atcatgccgg agaatgctta cgtcagcttg cggcattaaa
      361 cagtcgatta aactgcgcac aagagatggc ccggcgcgac agtgttggtg aagttcctcc
      421 tgtgccgtgg cgcacggtcg ttggctctgg cattgccgga gaagcaaagc ttgatcatct
      481 gcggctggtg tctttaggta tgcgttgctg gcaggatatt gagcattatg gtttacgcat
      541 ctggtttacc gatcccgaca ccggcagtat tttgcatctt tcgcgtagtt ggccgcgaag
      601 tgaacaggaa aactcaccgg cagctacgcg tcggctgttt agttttcagg ctggcgcact
      661 ggcgggtggg caaattgttt cacaagcagc aaaacgcagt gccgatggcg agctgctgtt
      721 agctacccgc aaccgcttaa gcagcgttgt gccgctgtcg ccagacgcct ggcaaatgtt
      781 gagcgcgccg ttacgccagc cgggcattgt ggctttgcgg gaatatttac gccagcgtcc
      841 ccccgcctgc atacggcctc ttaatcaggt ggataactta tttattctgc cggtcgctga
      901 gtgtatttca ctcggttggg acagcggccg ccagacgctg gatgcgcagg taatcagcgg
      961 cgaaggtgaa gataatctgc tgacgttatc ccttccggca tctgcctgtt ctccttttgc
     1021 cgttgaacgc atggcggcgc ttttgcaaca aacagacgac cccgtgagtc tggtttctgg
     1081 ctttgtcagc tttgtagagg ggcaattgac actggaacca cgggtgatga tgacaaaaac
     1141 ccgtgcctgg gcgctggacg cggaaactgc gcctgtggca ccgctacctt ctgccagcgt
     1201 tttgcctgtg ccgtctaccg ctcatcagtt gctgatgcgc tgccaggcgt tacttattca
     1261 actgctccat aacggctggc gctatcagga acagagcgct attagtcagg cagagttgct
     1321 ggcgaatgac ctcaccgcgg tgggttttta tcggctggca catgtgttgg tacaatttcg
     1381 taatacagaa agcgaggcac gggtagaagc aatgaataac ggtgttttgc tttgcgaaca
     1441 attattcccc atgcttcagc aacaaggatg aaatagtgct ttttactaag agttctactc
     1501 cagttccgga ctgctcacgc cacggtatta ggcatatcct atatagcccc tggtgagagt
     1561 caccagtttc ttgattaaat aaaatggagt tttacatgaa ggctttcaat aagctgtttt
     1621 ccctcgttgt tgcatctgtt ctggttttct ctcttgctgg ctgcggtgac aaagaagaat
     1681 cgaagaaatt cagcgccaat ctgaacggca ctgaaattgc cattacctat gtctacaaag
     1741 gtgacaaggt gcttaagcaa tcttctgaaa ccaaaattca atttgcctcc attagtgcaa
     1801 ccaccaaaga agatgctgcc aagacacttg agccgttaag cgccaaatac aaaaacatcg
     1861 cgggtgttga agaaaaatta acctataccg atacctacgc gcaggaaaac gtgactatcg
     1921 atatggaaaa agtggatttt aaagccctgc agggtatttc aggaatcaac gtttctgctg
     1981 aagatgccaa aaaaggtatc actatggcgc aaatggaact ggtgatgaaa gccgctggtt
     2041 ttaaagaagt gaaataatct gtcggcggcc atgtttcgta tggccgccat acccgtctta
     2101 gcttttcttc acatgctggc gcgctgccag tccacgcaga aaataacgta aaaattgatc
     2161 gccgcattcg cggaagtttt tatgatccgg tgcgcgcatc atcgctgtaa tttccggcat
     2221 cgaaacgcgg aactgctgtt cggtgaggat agccagaatg tcatcggttt tcagcgaaaa
     2281 cgcgatgcgt aattttttca gcacgatgtt gttattaatg cgacgttccg gctccagtgc
     2341 cggagcagac tcatccttgc cgcgtttttc ataaatcagg ccattgagga atgacgacaa
     2401 aacaatgtcc ggacaacgct gaaaaccctc ttcgtcttct ttacgtagcc agacggcgat
     2461 ctgttccgcg gtggcttcga cattacccag cgccagaata cgcaccaggt cattattatt
     2521 ggctttcaaa atgtagcgca cgctgcgcag aatatcgtta cttagcatga ggccttcagg
     2581 tgttgatgag gcaaaaagcc attttagcag tcttttacag gccaatcgcc tcttttaagc
     2641 ttttcagata acggcggctg accggcacgg ttaagccatt acgcaaaatc aactcggcct
     2701 ggccgttatc ttccagacga atctcctgta aatgcgcgag gttaaccaga tactgacgat
     2761 ggcagcgcag tagtggtgta cgactttcca gggtacgtaa tgtcaattcg gtaaagccct
     2821 ctttcccttc gtggctggta acgtagacac cgctcatccg actgctgaca aatgccacat
     2881 ctttcatttg cagcaaataa atccgactat gccccgtaca agggataaat ttcagcgcct
     2941 gttgattttc cggtaacagc gaaacatcct gcttgctgcg ctcctgacgc aatcgcgcca
     3001 gcgttttctc cagtcgcgct tcatcaattg gcttcagcag ataatcaaag gcatgttctt
     3061 caaaggcttt aatagcgtat tcgtcaaacg cagtgagaaa aacaatatac gggcgatgtt
     3121 ccgggtcaag catccccacc atttccagac cactgatgcg cggcatctgg atatcgagaa
     3181 acagcacatc cgggcgcagt ttatgcaccg cgccgatccc ttccacggcg tttgaacact
     3241 ctccaacgat ttcaatatcg ctctgctcct gcaaaaatac acgcaggttc tcccgtgcta
     3301 acggttcatc atcgacaatt aagactttaa tcatgcctcg tccctccatg gtagttgtaa
     3361 cgttattcgg gtgtaactat caggctcaca ggcgacgctt attccatagt catcgccaaa
     3421 ccgttcacgt aaacgcttat ccaccagatt catccccagc ccactggcat tggttaccgg
     3481 ttgatacaaa ccggcattgt cttcgatctc cagcatcaaa tgttgcccct cacgtcgggc
     3541 gctgattgcc actcgccctg tatccagcag ttgtgatgtc ccatgtttaa tggcgttttc
     3601 cactatcggt tgcagggtaa acgcgggcaa ttgctgctgg gataattctt gcggaatagc
     3661 aatgttgacc tgcaaccgcg actggaagcg cgccttttca atttgcagat aagcgttcac
     3721 atgttcaatt tcgtcggcga gagtaacaaa ctccgaaggc cgctttaagt ttttgcggaa
     3781 aaaagtggaa agatactgca ccagctggct ggcctgttcg ctgtcgcggc ggatcaccgc
     3841 tttaatggtg ttaagcgcat taaacaaaaa atgaggattc acctgggcgt gaagcagttt
     3901 gatttctgac tgggtgagca tcgctttttg ccgctcatat tgcccggcaa gaatctgcgc
     3961 cgaaagcagt tgcgcaatcc cctcgcccag cgtgcggttt attgaactga ataaacggtt
     4021 tttggcttca tacaatttga tggtgcccat cacccgctga ttttcaccac gcaacggaat
     4081 taccagcgtc gaccccagtt tgcattgcgg atgcaaagag caacgataag gtacttcgtt
     4141 gccatcagcg tagaccactt caccggtttc aatcgctttt aaggtgtaag tcgaagaaat
     4201 cggtttgccg ggtaaatggt ggtcgtcacc aattccagta aaggccagca atttctctcg
     4261 atcggtaatc gcgactgcac caatatccag ctcctgatac agcacctgtg ccactttcat
     4321 gctgttcact tcgttaaacc cctggcgcaa aatgccttcc gtcgaggcag ccactttcag
     4381 cgcagtggca gaaaaagccg aagtgtattt ttcaaacatc gcgcgtttat cgagcaatat
     4441 acgcataaac agcgccgcgc cgacggtatt ggtgaccatc attggtgcag caatattact
     4501 caccagacgc accgcatctt cataaggtcg ggcgatcgcc aggatgatca acatttgcac
     4561 catttcagcg acgaacgtga cggcaccggc ggtaatgggg ttaaagactt tatcagtacg
     4621 cccgcgacga atcaggatgc tgtgtaccag gccgccgagc aatccttcaa cgatggtcga
     4681 gatcatgcaa cttagcgcgg tcatgccccc catcgaatat cgatgtaacc cgccggtcag
     4741 gccaaccagc ccaccgacga ccggaccgcc gagtaagccg cccattaccg cgcctatcgc
     4801 acgggtattg gcaatagaat cgtcaatgtg caacccaaac caggtgccca tgatgcagaa
     4861 gatggaaaag acgatgtagc agagaaattt atgcggcaga cgaaccgtga cctgcattaa
     4921 cggtatgaat aatggcgttt tactcattaa ccacgcaatg actaaaaaaa cgcacatctg
     4981 ctgaagcagc agcaacacca gattaaaatc gtacataccc gcaaaccaca cttcccttta
     5041 aaacgcgtaa catacattgc ctgcgtttaa ctttctttga actcttgcag aaaaatgaga
     5101 attcgtgagt acgatcactc aaaatcgcct ggcaaaaata aaatcaccct atagatgcac
     5161 aaaaaacggg caaaactacc tggttcgcaa aactgcgtct aaagttaaac cgggacctcg
     5221 cgagcaaggg tgagacgatg gcgctttaca caattggtga agtggcgttg ctttgtgata
     5281 ttaaccctgt cacgttacgc gcgtgacgca acattttaaa aaatcgacga agatcgtcca
     5341 ggagcgccgt ttttcaaggg ttggatagat gattcgttta ccatgtcgac gaacttcgac
     5401 gaatttcgcc gataaaaagt gtcccataca tgccccattg atgccccata aacaatatca
     5461 gaaattgata ctggttatcc atacagataa aaataacacc tgtacataac tacagttaca
     5521 ggagggtgtt atgcgtgttg agatctgtat cgctaaagaa aaaatcacta aaatgccaaa
     5581 cggtgctgtg gatgcgttaa aggaagaatt aacccgacgc atcagtaaac gttatgacga
     5641 tgtagaggtg atcgtaaaag ccaccagcaa cgatggcctt tctgttacac gcaccgcaga
     5701 taaggattct gcaaaaacat ttgttcagga gactctgaaa gatacctggg agtctgctga
     5761 cgagtggttt gttcactaat aaacacgtaa aatcggtaac ggctggaaat cattcaatac
     5821 tcgcactatc gaaagtttac cagccaaccg cagcacgtcc tgcatacgtc gtgtctgcgg
     5881 tttttctttt tcgcttacat tgtgtctggt tcttccggcc actcaatatc aggtgcagtt
     5941 gatgtatcaa cacagttcag caacacccga tacttcttcc aggcttccag caacgagttt
     6001 tcttcctccg ttgcgatctc cagatctaca gcatcctgaa gtggcgcaat atgctcactg
     6061 aattcctgga tgtagaactg tgtggtgacg gtcttccagc cattcggctc ctgctgtatc
     6121 gaagcatacc aggctatttc aatatcgcta tgctgcagca gcatttaacc ccttgtaatt
     6181 catcgccata attgatttaa ttcacaaata aaactataat atggtgaaat taatgaaaaa
     6241 aaacacagat gatggggcta aaatttacac accaattacc ctaaagcttt atgactggtg
     6301 ggttttggga gtatcaaatc ggcttgcatg gggatgtcct acaaaggaac accttcttcc
     6361 acactttctg gaacatttag gtaacaacca tctggatatt ggtgttggaa ctgggtttta
     6421 ccttactcac gtacctgaga gtagtctgat atctttaatg gatttgaacg aagctagcct
     6481 gaacgcggca tctacaaggg ctggggaatc aaaaattaaa cataaaatta gccatgatgt
     6541 ttttgaaact tatcccgcgg cgttacatgg tcaatttgat tccatttcca tgttttacct
     6601 tcttcactgc ctgcctggaa atatatctac aaaaagctgt gtaatacgca atgcggcgca
     6661 ggccttaact gatgatggaa ctctatacgg agccacaatt cttggcgatg gagttgtgca
     6721 caatagcttc ggtcaaaaac tgatgcgcat ttacaatcag aaaggcatct tttcaaacac
     6781 aaaagattcc gaagaaggct taacacatat actctcagag catttcgaga atgttaaaac
     6841 caaggttcaa ggtactgtag taatgttttc tgcttcaggg aaaaaatagc atccaaccgc
     6901 agcacgttct tgcttaagac gtgctacggt ttcctttacg atttggggtt gcgactttac
     6961 ctctattgat aatgcattcc ggcagaacat tcaaatatga atgtacgata tttaactaac
     7021 cgaaaaacaa aatatagcac aggcgagaca tttaccatct aagaaaatag tatcgttttt
     7081 ttacagttaa atcaacatca cttccttaaa atgaaaacaa taaatttcaa tcctgaaaca
     7141 ttttatttta gcttattatt tcccagtatc acattttatc aggatatcct tctgcgaggt
     7201 tattatgctt cctgtaaata atccccccct atccactgga aacgtctctt tttacagaac
     7261 tacatcaatc gacaatgttc acaataatta tctctccgaa tgggttgaat ggactaaaaa
     7321 cagcatttcc ggagaaaaca gggaaactgc ttttacccgg ctccaattat gtctggaaaa
     7381 cagtgaaaca tcgttggact tatcttgttt aggtctcaga tctctaccac gattgcctga
     7441 caatcttgat gaaattaatg taagcaataa ccaactatca atgctccccg agctaccaag
     7501 ggcattgaaa gaactgaatg caagcagtaa tcaattatct gcacttcctg aattaccagt
     7561 gtcgctggaa tatataaatg tgagtgataa ccatttgttc gcacttcctg aattacctgc
     7621 gtcactagaa tatattaacg taagtgacaa tcacctgtct gtacttccga ggttaccaat
     7681 gtcattggaa ttacttgatg cagccagaaa tgctttggaa gtaataccag attttccaga
     7741 aagagatgat catattataa gaatattctg gcttaatcag aaccggatca cggcaattcc
     7801 ggaaagcata cttggcctca gttctgatag cgttgtcaat cttagagaaa atcaactatc
     7861 tcccagaata atgcaaactt tgttacaaca aaccgccca
//
LOCUS       DAJVUJ010000176         7820 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.176, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000176 DAJVUJ010000000
VERSION     DAJVUJ010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7820
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.176"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            270..413
                     /locus_tag="OQQ48_003631"
     CDS             270..413
                     /locus_tag="OQQ48_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HCS1954255.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            426..635
                     /gene="cspE"
                     /locus_tag="OQQ48_003632"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             426..635
                     /gene="cspE"
                     /locus_tag="OQQ48_003632"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCS1954256.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            801..1610
                     /gene="rlmA"
                     /locus_tag="OQQ48_003633"
     CDS             801..1610
                     /gene="rlmA"
                     /locus_tag="OQQ48_003633"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HCS1954257.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDDKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTFCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1607..2173)
                     /gene="mntP"
                     /locus_tag="OQQ48_003634"
     CDS             complement(1607..2173)
                     /gene="mntP"
                     /locus_tag="OQQ48_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HCS1954258.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(2601..3059)
                     /locus_tag="OQQ48_003635"
     CDS             complement(2601..3059)
                     /locus_tag="OQQ48_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HCS1954259.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDNLERIYKLIVLTQ"
     gene            complement(3114..3965)
                     /gene="manZ"
                     /locus_tag="OQQ48_003636"
     CDS             complement(3114..3965)
                     /gene="manZ"
                     /locus_tag="OQQ48_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HCS1954260.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(3978..4778)
                     /gene="manY"
                     /locus_tag="OQQ48_003637"
     CDS             complement(3978..4778)
                     /gene="manY"
                     /locus_tag="OQQ48_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HCS1954261.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(4841..5812)
                     /gene="manX"
                     /locus_tag="OQQ48_003638"
     CDS             complement(4841..5812)
                     /gene="manX"
                     /locus_tag="OQQ48_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HCS1954262.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            6275..>7820
                     /gene="yoaE"
                     /locus_tag="OQQ48_003639"
     CDS             6275..>7820
                     /gene="yoaE"
                     /locus_tag="OQQ48_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HCS1954263.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIVKE
                     QPAHDED"
BASE COUNT         1987 a         1913 c         1941 g         1979 t
ORIGIN      
        1 acgcactatg taataacttc tcccactggc ctggaacaac tgaacttatt gaactatgtt
       61 agaaaatacg ccagtttaag tatctgcctg aactggcaag gttaagcaca atgatatatc
      121 ggcgcgtatt ccgttgcata agtgtgcaaa aaaagtggaa gacgtatcga gatttgtgcg
      181 tctgatcgag acatgtttaa aaatggcttg ccataattaa cgttgtatgt gataacagat
      241 ttcgggttaa acgaggtaca gttctgttta tgtgtggcat tttcagtaaa gaagtcctga
      301 gtaaacacgt tgatgttgaa taccgcttct ctgccgagcc ttatattggt gcctcatgca
      361 gtaatgtgtc agttttatct atgttatgcc tgcgggcgaa gaaaacaatc taaggaattt
      421 ttcaaatggc aaagattaaa ggtcaggtta agtggttcaa cgagtctaaa ggttttggct
      481 tcattactcc ggctgatggc agcaaagacg tgttcgtaca cttctccgct atccagggta
      541 atggcttcaa aactctggct gaaggtcaga acgttgagtt cgaaattcag gacggccaga
      601 aaggtccggc agctgttaac gtaacagcta tctgatcgaa tccactgatc tgaagtgtga
      661 atacgcttca atctcgctat aaagcctcgt cgaatgcgag gctttttact atgctttatc
      721 ttcgctcctg gcgttcggat atttgcccgc cgcgtgattc gcgttacact tgcggccttt
      781 agtatcctgc cggagttgtc atgtcttttt cctgtccact ttgccatcag cctctttcgc
      841 gtgaaaaaaa cagctatatc tgtccccagc gacatcagtt tgatatggcg aaagaagggt
      901 atgtcaatct gctgcccgtt cagcataaac ggtctcgtga tccgggcgac agcgcggaaa
      961 tgatgcaagc acgccgcgca ttcttagatg ccggacatta tcagccgctg cgtgatgcaa
     1021 ttgtcgccca actgagggaa cggcttgatg ataaggccac ggcggtgctg gatattggct
     1081 gtggtgaagg gtattacaca cacgcatttg ccgatgcgtt gcccgaaatc accacgtttg
     1141 gtctggatgt ttcgaaggta gcgataaaag cggcggcgaa acgctatccg caggtcactt
     1201 tttgtgtcgc ttccagccac cgtttgccgt tttccgatac cagtatggac gccataatac
     1261 gtatttacgc gccgtgtaaa gcagaagaat tagcacgagt agtgaagccc ggcggctggg
     1321 tcattactgc cacgccggga ccgcgacatt tgatggagct gaaggggctg atttacaatg
     1381 aagtacatct tcatgcacct catgcagaac aactggaagg ttttacatta cagcagagtg
     1441 cggagttgtg ttatccgatg cgtcttcgcg gtgatgaagc tgtcgcatta ttgcagatga
     1501 cgccgtttgc ctggcgtgcg aagccagaag tctggcaaac actggcagca aaagaagtgt
     1561 tcgactgcca gacggacttt aatattcacc tctggcagcg ttcttattaa ccgtggaagt
     1621 gcgtccagag gatctggacg ccgatgccga tcagtaccag cccgccgaga atttccgctt
     1681 ttttcccaat aattgagccg ataaagcgac caaccatcat ccctaatgtt gacataatca
     1741 aggttgcaca accaatggcc aatgcggtcg cgataatgtt gacctgcagg aaagcaagac
     1801 caacacccac agccatggca tccaggctgg tggcaatcgc ggtggttacc agtagccaga
     1861 aaccgtgtcg acggcgcggc tcttcatctt catcatctgc gccacgaaaa ccctcaataa
     1921 tcattcgccc gccgaggaat atcagcagca caaacgcaat ccagtggttc cattcaagga
     1981 caaaccggct ggctaacatg cccattcccc agccgatcag cggcgtcagg gtttcgacgg
     2041 caccaaaaat aaggccggtt cgcaatgctt cagaaaattt cggtttatgg agggtggcac
     2101 ctttaccgat tgatgcagca aatgcatcca tcgacatacc aaacgcaaga agaacagtag
     2161 cagtgatatt catgacaatg tcctgaccgg ggtatccata aaacacatca tcgcccccag
     2221 caaacatgca tacatgaaca acaagccgtg atggtttttg cgttcatgca ggcatagcga
     2281 ttgatgtgca tatggtctcg cctgactgaa agtattcagt ccgtacgtgc cacgttttgc
     2341 aacgagtatg ttgacacgta catttccgga atctggaaat cggctactcc ccaaatgagg
     2401 gcgcaacctt aacatatttt gagaatataa aacaactgtt ggaatatatt atttcattgc
     2461 tttattgata acgaattcat ttagatttat atatcaatga aacgttaaaa taaataacaa
     2521 ataagggaag aggtaatata gcctaagcta tgtctgatta ttaaaaatac actaaacata
     2581 gctttggcta aattcataac ttattgagtt aaaacgataa gcttataaat tctttccaga
     2641 ttgtcgatat ttcgaacgcg gattaacagc cgacgctgtt ctaattgcat caccaacacg
     2701 ccatcttccg acaagttcat cgctttgatt cggctatatt caatccagac attggcgaag
     2761 aaaaaacctt tttgtttaaa gatgatcttc ggaacgcgga tccagaatat ataaaaaccc
     2821 atcagagcca gtgcgcttaa taaccatgtc gttattaatg caccatgatt cgtgacgttg
     2881 ttatagataa gaatgacaat caatccgacg aagataacgc tatcgatgcg accacgccgg
     2941 agcaaaggaa ttgccagcag ggtggggccg ttacggcggg gcatgatgaa ctgatcgtag
     3001 atcgcgaagg ccaggagtgc ggcgatgaaa agaatcagta ccaggtccgt gattgtcatt
     3061 aatcctccag ataaaaaaac ggggccaaaa ggccccggta gtgtacaaca gtcttacagt
     3121 cccagcaggc cgcaagcgta accagcgata ccgatgacga agaagccaac gatgatccac
     3181 agcgggttaa cttttttgcg cagtagccac atacaagcaa aggtcagcag cagtggtacc
     3241 aggcctggca ttaactggtc cagaatagtc tggacagtag taacgtgttc tttgcccgtc
     3301 tggtcagtaa tgcgagagac aaccagcggg atgttgacat gtgtccactt gttaaccaat
     3361 gcccccatga caaacaggcc gaggatagac gccccttccg tcagtttttg caggaagcca
     3421 ccacccatat ctttaacgat atcgatacct ttggagtaac catacgctac gccgtagtaa
     3481 cgggttgcca gacgcaccag gttaaacagg atgaagaaca gcagcggacc taacaggctg
     3541 ccgctcatcg cgataccggc acccagtgct gcaaataccg gacgtacggt tccccagaag
     3601 atcgggtcgc ctacaccagc cagcggcccc atcaaaccga ctttgatacc gttgatagca
     3661 ccgtcgtcga tctctgcgcc attagcacgc tgttcttcca gcgccagggt tacgccgaga
     3721 atcggcgcag ccacgaacgg ctgggtgtta aagaactcca ggtgacggcg aatagcttgt
     3781 ttacgagctt cgttgttctc agggtagagg cgacgaattg ccggtaccat agagaagcag
     3841 aaacccagtg cctgcatacg ttcgaagttc catgaaccct ggaagaggtt agaacgcagg
     3901 aagacgccac gaatatcact ttgagtgagt tttttctcgg tggtagtttg agttgtatca
     3961 accatttcgc tcacctgtta gtccagttcg ttatcgagat cgttgttacc agcagcctga
     4021 gcaggcgcac cggctacgcg gttgtatttc gggctaagtt ggatgtagag cactgccata
     4081 acagtaccga tcacacccag agcaaccagg ttaaagttgg tgaatgctgc ggttacgaag
     4141 ccgaggtaga agaacggcat caggtagcca gcacgcatca tgttgataac catcgcataa
     4201 ccaaccacca cgatcatgcc accggcgata ttcagaccat tggtcaccac ttccggaatc
     4261 gcattcagca tgttctgtac ttcgctggta ccaacagaca gcgcaacgat gacggccgga
     4321 atagccacac gcattgcttg caggaacaga gaagaaacgt ggatccagga aatcgctgtc
     4381 aggttgccgt tatcagcagc cttatccgca gcgtgctgga aagcaacggt aatagtacga
     4441 acgatgatgg tcagtacctg gcccgcagcg gccagaggga ttgccagtgc gatacctgca
     4501 ccaatgctct gatgacctgc gataaccaga atggtagaaa tgatagaagc cagagcggcg
     4561 tcaggcgcaa ctgcagcacc gatgttcatc cagcccagcg cgatcatttc cagcgtacca
     4621 ccgataataa taccggtttt catatcccca agaacgatac ccaccagggt acacgcgatt
     4681 agcggacggt gaaactgaaa ttcatcgagg attgatccca tacctgcgat acaggctacg
     4741 ataaatacca gcacaatttg aagagtggta atctccattg tacttctcct gtttacgtaa
     4801 gacttaagtg tgaaaactga gtgataatca acacaatacg ttacttatcg attttgctga
     4861 tcagatccat cattttcagt ttcggatcgg tggaaacctt acggacttcc agctcaatac
     4921 cgcgcgcatt cagtttcttg aacgcctcga tatctttttc atcaaccgaa accgcgttat
     4981 tcacctgggt tttaccctga cggaatgcca taccaccgac gttaacagag gtgattttca
     5041 cgccgccttc aacgagacgc tctacatctg ttgggttggt aaataacagc attacgcgtt
     5101 cgccagcata tttcgggttg ttgtagacgc gaatcatttt ggcaacatca actacgtgtg
     5161 ctgttacgcc cggaggtgca acctgggtga gcagtgtctt acgaacggta tccgcagcca
     5221 cttcatcact aacaacaata atacgggaga cattggtttc tttggtccag cgggtggcga
     5281 cctgaccgtg aatcagacgg tcgtcgatac gcgcaaggcc aataaccatg tagtcgtttg
     5341 gccccattgg ttttgccgga gttggagccg cttttggtgc tgcggcagcg ggtgctggcg
     5401 cggctttttc aaccggtttg gctttcagtg ctttcacgcc ttcacggcct gtttctactg
     5461 ccagcgcaac cagttcatca aagcttgggt catcatcacg agccattaac gtttccacga
     5521 gcattggaat gttaacgcct gcaatgactt cataatgctc tttgtcgacg acaatgcggc
     5581 tggcagcatt gaacgggctg cctccccatg tatcaacgag aaacagcacg cctttagtgg
     5641 tgtcgagttt tgccaactga gcgttgtact tttcaatcag cgtttcggca ttttcacctg
     5701 gaacgaaatc gatccagccg acgttttcct gctcgcctaa cagcatttct gccgttttaa
     5761 gcaactgctc tgcagcccaa ccatgtgtgc ctataacaat agcaatggtc acttgctacc
     5821 tcctttatta tcgttaacac ctcaacgtgc cagatgtatt tttgaatcgt cgtccacaat
     5881 cgaatcgatt cagataaggg ctacaaccaa aaacccctgc gtttcgcgat ttattttaga
     5941 tatcgaaaaa attattttat gtgatgaaga tccgtaattt aactttcgat tagcagaaat
     6001 ttcgaaaggt aaaatatcct tgtcacattc gtttgcaaag gaaggtaaat ctttgccaaa
     6061 tcagaggcgt ctctgatatg tttaactccc gtttaacaac catggagtat agggcagtag
     6121 cccgcagtat ggatcgtcac cgacgtcatt tcagcatcag gccttttaac gcctgccttt
     6181 ctggcactct atgccgcacc tttcgtttgc attttgtcgt tacgcctgca ttatttctgg
     6241 cgtcgaatag ctattcctta agcaggagct tgtcatggaa ttcttaatgg acccctcaat
     6301 ttgggcgggg ctactcacgc ttgttgttct cgaaattgtg ctgggtatcg ataacctggt
     6361 cttcatcgcc attcttgctg acaaactgcc gccaaaacaa cgcgataaag cgcgtttgct
     6421 ggggttatca ctggcgctga ttatgcgtct ggggctgctg tcgctgattt catggatggt
     6481 cacgctgacc aaaccgctat ttaccgtcat ggatttctcc ttctccggac gcgacctgat
     6541 tatgttgttc ggggggatat tcttgctgtt caaagcaaca accgaactgc atgaacggct
     6601 ggaaaaccgc gatcatgatt ccggccacgg taaaggctac gccagtttct gggtggtcgt
     6661 cacacagatt gtcatccttg acgccgtctt ctcgttggat gcggtaatta ctgcagtagg
     6721 gatggttaac catctgccgg tgatgatggc ggcggtagtg attgcgatgg cggttatgtt
     6781 gctggcatcc aaaccgctga cgcgattcgt taaccagcac cccacggtgg tggtgctctg
     6841 tctgagcttc ctgttaatga ttggtcttag tctggtggca gaaggtttcg gtttccacat
     6901 tccgaaaggt tacctgtatg ccgcgattgg cttctcgatc atcatcgaag tgtttaacca
     6961 gattgcgcgt cgcaacttta ttcgccacca gtcgactttg ccgctgcgag cgcgtactgc
     7021 cgatgccatc ctgcgtttga tgggcgggaa acgtcaggcc aatgttcagc acgatgccga
     7081 taacccgatg ccgatcccgg aaggtgcatt tgccgaagaa gaacgttaca tgattaacgg
     7141 cgtactgacg ctggcgtcgc gttctctgcg cgggatcatg acgccgcgcg gtgaaataag
     7201 ctgggttgac gctaatctcg gggtcgatga aatccgcgag caactgctct cttcaccgca
     7261 cagtctgttc ccggtatgtc gcggtgaact ggatgaaatc atcggtattg tacgtgctaa
     7321 agaactgctg gtggcgctgg aagagggcgt tgatgtggcg gcgattgctt cggcgtctcc
     7381 ggcgattatc gtcccggaaa ccctcgatcc gatcaacctg ttgggcgtgc tgcgtcgtgc
     7441 tcgcgggagc tttgttatcg tgaccaacga gtttggtgtg gtacaaggtc tggtcacgcc
     7501 gctggatgtg ctggaagcca ttgcgggtga attcccggac gctgacgaaa cgccggaaat
     7561 cattactgat ggtgacggct ggctggtaaa aggcggtaca gatttgcatg ccttgcagca
     7621 ggcgcttgat gttgagcacc ttgccgatga cgatgatatc gcgacggtcg cgggcctcgt
     7681 gatctcggca aatggtcaca ttccccgtgt gggcgatgtg attgatgtag ggccactgca
     7741 tatcaccatc attgaagcca atgattatcg tgttgatctg gttcgcattg ttaaagagca
     7801 accggcgcac gatgaagatg
//
LOCUS       DAJVUJ010000177         7817 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.177, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000177 DAJVUJ010000000
VERSION     DAJVUJ010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7817
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.177"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(439..1173)
                     /locus_tag="OQQ48_003640"
                     /pseudo
     CDS             complement(439..1173)
                     /locus_tag="OQQ48_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000016737.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(1332..1691)
                     /locus_tag="OQQ48_003641"
     CDS             complement(1332..1691)
                     /locus_tag="OQQ48_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004983905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1954264.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLFRQ"
     gene            1798..2691
                     /locus_tag="OQQ48_003642"
     CDS             1798..2691
                     /locus_tag="OQQ48_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1954265.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPGVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRAQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPASRNFRIHLLNALR"
     gene            2826..4046
                     /gene="mlc"
                     /locus_tag="OQQ48_003643"
                     /gene_synonym="dgsA"
     CDS             2826..4046
                     /gene="mlc"
                     /locus_tag="OQQ48_003643"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HCS1954266.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNLGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPTYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLHG"
     gene            4171..4866
                     /gene="bioD"
                     /locus_tag="OQQ48_003644"
     CDS             4171..4866
                     /gene="bioD"
                     /locus_tag="OQQ48_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCS1954267.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(4819..6111)
                     /gene="clcB"
                     /locus_tag="OQQ48_003645"
     CDS             complement(4819..6111)
                     /gene="clcB"
                     /locus_tag="OQQ48_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HCS1954268.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTEMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(6270..6884)
                     /gene="dmsD"
                     /locus_tag="OQQ48_003646"
     CDS             complement(6270..6884)
                     /gene="dmsD"
                     /locus_tag="OQQ48_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HCS1954269.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQYEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELSRLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(6927..7781)
                     /locus_tag="OQQ48_003647"
     CDS             complement(6927..7781)
                     /locus_tag="OQQ48_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HCS1954270.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQRIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCASLGCWLCPLIRRREPHVAGLILGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
BASE COUNT         2094 a         1980 c         2013 g         1730 t
ORIGIN      
        1 taatactttt ttcattgtca taccctcaat ttgttttttc atttaacccc actgcggggc
       61 cgttgaaata actatatccc tgggaatggc agacttccgt gagtggttgg tttcagcgtg
      121 taacgatatg tacaaacgct gaataaatta cagcattgat aataacgtat tgtgacttaa
      181 gggaaattta gctaccaata atagtagtct tgatcgggtt aactatttta ctacttacaa
      241 agcggtagaa taactgcgca tcagtaataa atgacacaca gcaaaatgaa tccgtttatt
      301 tgggtactta taatcctgat gacgctagac gcactgcggg aattggctgg cgcttcgtct
      361 attttaggat ggctattaac gttggtttga gcaagcaata agtccggacg tgtagttacc
      421 gcagtccggg cttatttttc aggcgtgcag acgacgatgc aaacgcgtcc cgaccagcag
      481 agcaatgacc agcatcagag caataaatgc gccgacgccg ttccagccat agttatgcca
      541 gaaaacatca cccagcgtcc cggcaatact cgaccccaga tagtaactga acagatacag
      601 cgaggaggcc tggcctttag cgcgttttgc gcgggggccg atccagctgc tggcaactga
      661 gtgggctgcg aagaatcctg ctgagaagag taacattccg gcaaagatca gccacagcga
      721 gctgaataag gtcatcagta aaccaaacag cataaccccc gtcgaaaaca acatcaccgg
      781 accgcgcccg tagcgggtgg tcatggttcc ggctttgggt gagctccgtg taccggtcaa
      841 ataagccagc gataataagc caaccacggc ctgactgaca tgctaggggg aaagcatcaa
      901 ccgatagccg atgtaattaa acagcgtgac gaacgacccc atcagcaaaa agccttctgc
      961 gaacaataac ggtaatcccc ggtcacgcca gtgcagacga aagttgataa acaacgtctt
     1021 aggtcgcagc gaagtcgggc gaaaatggcg tgattccgga aggattttcc agaacatcaa
     1081 cgccgaggct agcgcgaaac aaccgattgc cgccagagca attcgccagt tgaaaaagtc
     1141 cgtgaagaca ccactaatta agcgtccgct catgccgcca attgagttgc cgctgatata
     1201 caaccccatc gaaaaggcca cgaaactggg gtggatttcc tcgctaagat aagtcatgcc
     1261 aacagctgcc acgccactta acgaaagccc aatcaaggcg cgcataatca aaatgccgtg
     1321 ccagctggtc atcattgtcg aaataacgta caaatggagg ccaacagtag cgccgtgacc
     1381 atcactggtt tgcgaccaat ggcatcggat agggggccag taaacagcaa accaatagcc
     1441 aacatcgccg tggaaatgga cagtgaaata ctactgttcg cgggggttaa gccaaactcc
     1501 tgcgaaagca ccggaaggat aggctgcaca caatagagga gtgcaaaggt tgccagtccg
     1561 gcagagaaca gcgccagggt gacgcgcata aattgcggcg taccgcgttt aataaattga
     1621 tttggctgag aaatgctttg cttgtcagtg tcgcttgccg gagcgccatc aacagttgta
     1681 gtacggctca cttgaaatcc ttgctaaata tgcctgtgga tcaggcttat acataggtta
     1741 ggaaaatcga attgttctgt ctaatatatt aataatctca aataagatgt tttaaatatg
     1801 aatattgaac ttcgtcatct gcgttacttt gttgctgttg cggaagagct gcatttcggg
     1861 cgcgccgctg cccgcctgaa tatttcgcaa ccaccgctaa gtcagcagat tcaggcactg
     1921 gagcaacaaa ttggtgcccg actgctggca cgaactaatc gcagtgtatt gctgacggca
     1981 gcaggaaaac agtttcttgc agatagtcgg caaatcctgt ctatggtaga tgacgctgcc
     2041 gctcgcgctg aaagactgca tcagggtgaa gcgggggagt tgcgcattgg ttttacttcg
     2101 tccgctcctt ttattcgggc ggtgtccgat acgttatcgc tgtttcgccg tgattatccc
     2161 ggtgtccatt tacaaacccg cgaaatgaac actcgtgagc aaatcgctcc gctcattgaa
     2221 ggaacgctgg atatgggatt gctgcgtaac acagcgttac cggagacgct tgaacacgca
     2281 gtcatcgtcc atgaaccgct tatggcgatg atcccgcacg atcatcccct ggcaaataac
     2341 ccgaatgtaa cgttggctga actggcgaaa gaacccttcg tcttttttga tccgcacgtc
     2401 gggacagggc tgtatgacga tattctcggg ctgatgcgac gttaccattt gacgcccgtc
     2461 atcactcagg aggtgggcga ggcaatgacc atcatcggtc tggtttccgc cggtctgggt
     2521 gtttcaattt tgcctgcttc atttaaacgt gctcagctca acgaaatgcg ctgggtgccg
     2581 attgctgaag aggatgcggt ttctgaaatg tggttggtct ggccgaaaca tcatgaacaa
     2641 agtccagctt cgcgtaactt tcgtattcat ctgctgaatg ctctcaggtg agggaaattt
     2701 cagcgaaaaa gcccgaaaaa tgtgctgtta atcacatgtc taagtaaaaa tttgacgaca
     2761 cgtattgaag tgcttcacca tagcctacag attatttcgg agcgcgaaaa tatagggagt
     2821 atgcggtggt tgctgaaaac cagcctgggc acattgatca aataaagcag accaacgcgg
     2881 gcgcggttta tcgcctgatt gatcagcttg gtccagtctc gcgtatcgat ctttcccgtc
     2941 tggcgcagct ggctcctgcc agtatcacta aaattgtccg tgagatgctc gaagcacact
     3001 tggtgcaaga gctggaaatc aaagaagcgg ggaaccgtgg ccgtccggcg gtgggactgg
     3061 tggttgaaac tgaagcctgg cactatcttt ctctgcgcat tagtcgcggt gagattttcc
     3121 ttgctctgcg cgatctgagc agcaaactgg tggtggaaga gtcgcaggaa ctggcgttaa
     3181 aagatgactt gccattgctg gatcgtatca tttcccatat cgatcagttt tttatccgcc
     3241 accagaaaaa acttgagcgt ctaacttcga ttgccataac cctgccggga attattgata
     3301 cggaaaatgg tattgtacat cgcatgccgt tctacgagga tgtaaaagag atgccgctcg
     3361 gcgaggcgct ggagcagcat accggcgtac cggtttatat tcagcatgat atcagcgcat
     3421 ggacgatggc agaggccttg tttggtgcct cacgcggggc gcgcgatgtg attcaggtgg
     3481 ttatcgatca caacgtgggg gcgggcgtca ttaccgatgg tcatctgcta cacgcaggca
     3541 gcagtagtct cgtggaaata ggccacacac aggtcgaccc atatggtaaa cgctgttatt
     3601 gcgggaacct cggctgcctc gaaaccatcg ccagtgtgga cagtattctt gagctggcac
     3661 agctgcgtct caatcaatcc atgagctcga tgttacatgg gcaaccgtta accgtggact
     3721 cattgtgtca ggcggcattg cgcggcgatc tactggcaaa agacatcatt accggggtgg
     3781 gcgcgcatgt cgggcgcatt cttgccatca tggtgaattt atttaaccca caaaaaatac
     3841 tgattggctc accgttaagt aaagcggcag atatcctctt ccctgtcatc tcggacagca
     3901 tacgtcagca ggcccttcct acgtatagtc agcacattag cgttgagagt actcagtttt
     3961 ctaaccaggg cacgatggca ggcgctgcgc tggtaaaaga cgcgatgtat aacggttctt
     4021 tgttgattcg tctgttgcat ggttaacatt ttttaactgt tctaccaaaa tttgcgctat
     4081 ctcaatttgg gccaggaaag cataacttag actttcaagg ttaattattt tcctagttta
     4141 tatttgtgaa gcataacggt ggagttagtg atgctgaagc gtttctttat taccggtaca
     4201 gacacttctg tagggaaaac ggtggtttcc cgcgcattgc tacaagcgtt agcctcccag
     4261 ggaaaaacag ttgcgggata taaacccgta gcgaagggga gcaaagagac acccgaaggg
     4321 ctgcgtaata aagatgccct ggtgttgcag agtgtttcaa ccatcgaact gccttatgaa
     4381 gcagttaatc ctatcgcgtt aagcgaagaa gaaagtagcg tggcgcacag ttgcccaatc
     4441 aattacaccc tcatttcaaa cggcctggca aacctgaccg ataaagtcga tcatgtcgtg
     4501 gtagaaggga ctggcggctg gcgcagtctg atgaatgatt tgcgtccact ctctgaatgg
     4561 gtggtgcagg aacagttgcc ggtgttgatg gttgtcggta ttcaggaagg ttgcattaac
     4621 catgcactgc taacagctca ggcgattgcc aacgacgggc tgccgctcat tggctgggtg
     4681 gctaaccgaa tcaacccagg actggcgcat tatgcggaaa tcattgatgt gctgggtaaa
     4741 aaacttccgg caccgctcat tggtgaactg ccttatctgc cgcgcgccga acagcgagaa
     4801 ctggggcaat acatccgctt agctatgctg cgcagtgtgc tggcggtaga tagagtcacg
     4861 gtgtaacgtc cgcgaaatta ccgacgcaat gacgcaggca atcaataaac cggggagtag
     4921 ctgatactcc ccggtcattt cacatatcat caacgtcgac ataatcggcg cgtgcgtggt
     4981 tgccgccaac agtgtcgcca tctcggtcaa tccgagtaaa agcgtaattt cttcgccatc
     5041 agggaaccat aatcccaggc tacgaccata caacatgcca atggcaagac cgataaatag
     5101 cgtcggtgta aagacaccac cgggtgcgcc ggaaccgcta ctcgccagca cggcacacag
     5161 tttacaaagg aagatcccgg caatgatcat taacagtggt ggggcggtta aaaaggattg
     5221 tacggtgcta tagccgttgc cccacactgc aggtgtgaac agggaaagca gacccacgat
     5281 caacccgcct agtgccagtt gccagggcgg cgcaagtttg agactcacaa atccacgatg
     5341 acaggcgttc attaacgtta acaacagtgg tccgcacaga cctgccagca cacctgtact
     5401 gataatcagc gcatagtcac gagcctgaac cgtcactgag agttgtacgt tgtagagtaa
     5461 cgcgtcgcta tgattaatca gattgctaac cagcaatgcc acgacggcgg aaataatcac
     5521 cgggccgaga gaggccaaca tcatagtgcc aaacagcact tcggctataa ataaactgcc
     5581 agcaagcggg gcacgatagg ccgcagccat tcccgccgcg gcgccacagg cgatccataa
     5641 tttccactcc tggcgtggcg taaaacgttg ggcaaaacag gaggcggcaa gggcagctaa
     5701 aagaatcatc gcaccttcgc gaccaattgc actgccgctg gttactacca gcagcgaggc
     5761 aagcgattta accaggcttg ctgcgtaatc gaactgtcca tcggtttgca acgcttccat
     5821 gtaatcggtc ggcgcatgag ggcgttgttg ggtaaatttc tgccagccca tcagcaacaa
     5881 acccgccgcc agtccgccga gcgccggagt tagcaaccgt cgccaggggg aaaggtttgt
     5941 cgctgcattg accagactgc cggagtcatt attgaggaac aaccactcca gtagcagcat
     6001 cgcatgacga aacccggcaa cggcaaaggc cgcgagaata ccgacgactg ttgcgataag
     6061 cagacggcgg aacatagtgc gcagatccgg atagatgtgt agatgatgca tgagcagaaa
     6121 tgggcaatga aacacgggcg actattttgc ggcaaaagag gccagccgca aaagcagtgc
     6181 gtgctttaag agataaaaaa acgtgacact gtaacctatt attgccggat tgcgcgtaat
     6241 cgtcaccatc cggcaatatt acggtgatcc tatcgaaaca gcggtttaac cgcgacagga
     6301 attaacagtt gtgattgcca ttgcgccagc gttaagcgag acagttcacc cagtgcacgg
     6361 tagaaagggt gttctgcttt ttcgataaaa acatcaagaa aacgtgttga ccacggaaaa
     6421 aggtgccatg ccagcaattc ttcgcattct gtctggcgac cattctccgc caaccacgca
     6481 gccatcaaca gcagtgaacc aaaatgatct tccggttcgt attgcttcat ttcaaactga
     6541 atgcctttct cgcgcatcca ctgacgaagt gccaatgttg aatcgccaaa cagcacagat
     6601 tcgcgatcca gccagaccga cccccacggc ggagacggca gtgcccacgg gccgacaaac
     6661 aaacgctgcc aggcctgggc gtgagtctct tcacactgtg tctgaaaagc cgtcaccagc
     6721 ggcgctaatg acgcttctgg tagaggccac tgagtttccc agccgtcact ggtgagtacc
     6781 gcaaccagag gtgcggcttc cgcactctct ggagcgtaat aaaacagcgc gcccaacacg
     6841 cgcgccgcga cagaaaaatt atcttgctgt gaaaaatggg tcattcctaa catcctgaaa
     6901 tgtgcgggtt gccccgcacc aatttgttaa cctgcaatag ccattcctac tgtcatgtgc
     6961 aggccataaa agagcacacg cccgatcatc tcaccgccaa gtatgaggat tagccccaga
     7021 ataagtccgg caacgtgagg ttcacggcga cgaatgagcg ggcaaagcca gcatccaaga
     7081 cttgcgcata acaataccac acgccagacc tgtaaggagg cgtaatctgg taccagtgcg
     7141 ctggcttgtt gcacggatga gtgaatagac gccagagaca aaccttgcag cacaatcacc
     7201 ccggcacaag cgatcagcgc cagaacgcta atgatggcaa atggtgtggt attaaatgta
     7261 acgcgagctg cccgcagaat tgcggcagca agtattggac cgctcaacaa taccgtcagg
     7321 aagaaagcca gcgtagtgta accgttatgc caggttggca cggtgtcgat ttgatacacg
     7381 caggtcatca tccagacgaa aatgacgccg agcgccatgc tgacaagtaa ccagagtttc
     7441 cccaacgctt gcggcatttt accgatgacc gccaccagcc accatagtcc accaacggca
     7501 aaaaatatgg aaccggcagc aatctcgtta ctcagaccag aagctccgat tcgattcagc
     7561 gagttaaacg ctcgcagtgg cgatcccagg tgcatgacag aggcaataaa cccgatgccc
     7621 atcaacagcc agaggaaaaa catgccacgg acaatacgct gacggtctgc gtcatttttg
     7681 gcggcgaacc agccaattcc gctaacaatc agcgcgccta ctacgcattg acccaatacg
     7741 gtaaagataa ccagtggcca ttcatgccat ccatttccca ttttacactt cctccggatt
     7801 agccagataa ccagtgg
//
LOCUS       DAJVUJ010000178         7772 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.178, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000178 DAJVUJ010000000
VERSION     DAJVUJ010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7772
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.178"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..925
                     /locus_tag="OQQ48_003648"
     CDS             <1..925
                     /locus_tag="OQQ48_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110-like element ISEc11 family transposase"
                     /protein_id="HCS1954271.1"
                     /translation="RRQDFLTFFGNREPCLIGMEACGGSQHWARELTKLGHKVRLLQA
                     RFVKAFVMGNKNDVMDARAIWMAVQQPGKEIAVKTEEQQSVLVLHRTRMQLVKFRTAQ
                     INALHGTLLEFGETIHKGRAAMEREFPEALERMKERLPPYLIMVLENQYNRLNELDSL
                     IEDIEKQLTSVARQNETCKRLLDIPGVGPLIATAAVATMGEASAFKSGREFAAYVGLV
                     PKQTGSGGKVRLLGISKRGDTYLRTLFIHGARAVALVAKEPGPWITELKKRRPASVAI
                     VAMANKLARTVWAITAHDRKYDRNHVSIRPY"
     gene            1209..1355
                     /locus_tag="OQQ48_003649"
     CDS             1209..1355
                     /locus_tag="OQQ48_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001163619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HCS1954272.1"
                     /translation="MQHGHIAQRGNHDELAQQSGWYRDMYRYQQLEAALDDAPEIREE
                     AVDA"
     gene            1348..3129
                     /locus_tag="OQQ48_003650"
     CDS             1348..3129
                     /locus_tag="OQQ48_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCS1954273.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKMVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            3310..3648
                     /gene="glnK"
                     /locus_tag="OQQ48_003651"
     CDS             3310..3648
                     /gene="glnK"
                     /locus_tag="OQQ48_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HCS1954274.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            3678..4964
                     /gene="amtB"
                     /locus_tag="OQQ48_003652"
     CDS             3678..4964
                     /gene="amtB"
                     /locus_tag="OQQ48_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HCS1954275.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLMQVTVTFALVCILWVVYGYSLAFGEGNNFFGNVNW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQELEGLDVNSHGENAYNA"
     gene            complement(5013..5873)
                     /gene="tesB"
                     /locus_tag="OQQ48_003653"
     CDS             complement(5013..5873)
                     /gene="tesB"
                     /locus_tag="OQQ48_003653"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HCS1954276.1"
                     /translation="MSQALKNLLALLNLEKIEAGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            6091..6663
                     /locus_tag="OQQ48_003654"
     CDS             6091..6663
                     /locus_tag="OQQ48_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCS1954277.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINKGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(6694..7005)
                     /locus_tag="OQQ48_003655"
     CDS             complement(6694..7005)
                     /locus_tag="OQQ48_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HCS1954278.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            7168..7264
                     /gene="ffs"
                     /locus_tag="OQQ48_003656"
     ncRNA           7168..7264
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="OQQ48_003656"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            7384..7737
                     /locus_tag="OQQ48_003657"
     CDS             7384..7737
                     /locus_tag="OQQ48_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HCS1954279.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
BASE COUNT         1777 a         1902 c         2253 g         1840 t
ORIGIN      
        1 gcgtagacag gattttctga cgttcttcgg caaccgtgag ccatgcctga ttggtatgga
       61 ggcctgtgga ggttctcagc actgggcacg ggaactgaca aaacttggtc ataaagtccg
      121 gttgttgcag gcccgcttcg ttaaggcatt cgtcatgggc aataagaatg atgtgatgga
      181 tgcccgggct atctggatgg cggttcagca gccgggtaaa gaaatcgccg taaaaacaga
      241 agaacagcag tcggtactgg ttctgcaccg tacccgcatg caactggtga agttccggac
      301 cgcacaaatt aatgccctgc acgggacgtt actggagttt ggtgaaacca tccacaaagg
      361 ccgggcagcg atggagcggg agttccccga agcactggaa cggatgaaag agagactgcc
      421 accgtatctc attatggttc tggaaaacca gtacaaccga ctgaatgagc tggactcact
      481 gatagaggat attgaaaaac agcttaccag cgtggcgagg cagaatgaaa cctgtaagcg
      541 gttgctggat attcctggcg ttggaccact tattgcgacg gcagcggtgg ccaccatggg
      601 ggaagcatca gcgtttaaat cggggcgaga gttcgccgca tatgttggtc tggttccaaa
      661 acaaactggc tccggaggga aagtacgtct gctggggata agcaaacgtg gtgacactta
      721 tctcaggaca ttatttatcc acggtgcaag agcggtggca ttagtagcta aagagcctgg
      781 cccgtggata accgaactga aaaaacgtcg tccagccagt gtggcaatcg tcgccatggc
      841 aaacaagctg gcacgaacag tatgggcgat aaccgcccat gaccgtaagt atgacaggaa
      901 ccacgtcagt atcagaccat attaatcgct gataccatta aacaatgaac tcttaacaaa
      961 agggtgaatg ctgaaaggtt gctatggcgg ccagagtgat gacaaagaca ggtaagaccg
     1021 tgactcacta aacctgaaca gtattttggg cttgaagtcc gccgtgaaaa taaggggtga
     1081 gtcggcgaat tacatagggg ctcgcagcgt tacggctgca ataaagccgg atataaagct
     1141 gcaacctacc cgtcatgtca aaacaatgga tgccttgcaa atgggatgcg ttcatataaa
     1201 ttattgtgat gcagcacgga catatcgccc agcgtggcaa tcatgatgag ttggcgcaac
     1261 aaagcggctg gtatcgcgat atgtatcgct atcaacaact ggaggcggcg ctcgacgacg
     1321 ctccggaaat tcgcgaggag gccgtcgatg cgtagtttta gccaactgtg gccgactctc
     1381 aagcgtctgt tagcgtacgg ttcgccgtgg cgtaaaccgc tggggattgc ggtcctgatg
     1441 atgtgggttg cggcagcggc agaagtcagt gggccgctgc ttatcagcta ttttatcgac
     1501 aatatggtag cgaaaaataa cctgccgttg aaaatggttg cagggctggc tgcggcgtat
     1561 gttgggctac aactgtttgc cgccgggcta cattacgcgc agtcgctgct gtttaatcgg
     1621 gcggcagtag gcgtagtgca acagttgcgt accgacgtga tggatgccgc attgcgccag
     1681 ccattaagcg agtttgatac ccaacccgtc gggcaggtga tttcccgcgt cactaatgac
     1741 actgaagtga tccgcgatct ctacgttacc gtagtggcaa ctgtcctgcg tagcgccgca
     1801 ctggtgggcg cgatgctggt ggcgatgttc agcctcgact ggcgaatggc gctggtggcg
     1861 ataatgattt tcccggtggt gctggtggta atggtgatat accagcgtta cagcacgccg
     1921 attgtccgtc gtgtgcgcgc ctatctggcg gatatcaacg acggctttaa cgaaatcatc
     1981 aatggcatga gcgttatcca gcagttccgt cagcaggcgc gatttggcga acgtatgggg
     2041 gaggccagtc gttcacacta tatggccagg atgcaaaccc tgcgcctcga cggttttctg
     2101 ctgcgtccgc tgctgagtct gttttcatcg ctcattcttt gtggcttgtt gatgctgttt
     2161 ggcttctccg ctagcggcac cattgaagta ggcgtgctgt atgcgtttat cagctatctt
     2221 gggcgactta acgaaccctt aatcgaactg accacgcaac aggcgatgct gcaacaggct
     2281 gttgttgctg gtgagcgcgt gtttgaactg atggacggac cgcgccagca atatggcaat
     2341 gatgatcgcc cgttacagag tggcaccatc gaagtcgata acgtgtcatt tgcttatcgc
     2401 gatgacaatc tggtgctaaa gaacattaat ctctctgtgc cttcgcgtaa ctttgtggca
     2461 ctggtcgggc ataccggcag tggcaaaagc accctcgcca gtttattgat gggctattac
     2521 ccgctaacgg aaggtgagat tcgccttgat ggtcgtccat taagttcgct aagtcacagc
     2581 gcgctgcgcc agggcgtggc aatggtgcag caagatccgg tggtgctggc ggataccttc
     2641 ctcgccaacg tgacgctggg gcgtgatatc tccgaagaac gcgtctggca ggcgctggaa
     2701 accgtgcaac tggcggagct ggcgcgcagc atgagtgatg gtatttatac gccgctgggc
     2761 gagcagggga ataatctctc cgtcgggcaa aagcaactgc tggcactggc acgcgtgttg
     2821 gtggagacgc cgcaaatcct gatccttgat gaggcaaccg ccagcattga ctccggtact
     2881 gaacaggcga ttcaacatgc tctggcggct gtgcgtgaac ataccacgct ggtggtgatt
     2941 gctcaccgct tatcgaccat tgttgatgcc gacaccattc tggtgcttca tcgtgggcaa
     3001 gccgtggagc agggcactca ccagcaactg ctggcggccc agggacgcta ctggcagatg
     3061 tatcaactgc aacttgcggg cgaagagctg gcagccagcg tgcgtgaaga agaatcattg
     3121 agcgcctgaa tagcgcaata tttcatcgtt ggtgcaaaaa tgtaacgcac tgtgcactgt
     3181 catagtgcgt tttcattttc aatcttctta acttcctgct ctctttctcg tttttcattt
     3241 ctggcacacc gcttgcaata ccttcttcgt gtagcagaac cattaccgaa ttctgactgg
     3301 aggggatcta tgaagctggt gaccgtgata atcaaaccat tcaagctgga agacgttcgt
     3361 gaagcgttat cttccattgg tattcagggc ctgaccgtca ccgaagtgaa aggtttcggg
     3421 cgtcagaaag ggcatgccga gctgtaccgg ggggcggaat acagcgtcaa cttcctgcca
     3481 aaagtaaaaa ttgatgtggc aattgctgat gaccaactcg atgaagtgat cgatatcgtc
     3541 agtaaggcgg cttacaccgg aaaaattggc gacggcaaaa tcttcgtcgc tgaattgcaa
     3601 cgcgtcattc gtattcgtac cggcgaagcc gacgaagcgg cgctgtaatc tctggcacac
     3661 agcaacagga acgaaaaatg aagatagcga cgataaaaac tgggcttgct tcactggcga
     3721 tgctgccggg actggtaatg gctgcacctg cggtggccga taaagccgac aatgcgttta
     3781 tgatgatttg tactgcgctg gtgctgttta tgactattcc ggggattgcc ctgttttacg
     3841 gtgggttgat tcgcggcaaa aacgtgctgt cgatgctgat gcaggtgacg gtgacgtttg
     3901 cactggtctg tattctctgg gtggtttacg gttactcgct ggcgtttggt gagggtaaca
     3961 acttcttcgg caacgttaac tggttgatgc tgaaaaacat cgaactgacg gcggtgatgg
     4021 gcagcattta tcagtatatc cacgtagcgt ttcagggatc gtttgcctgc attaccgtag
     4081 gcttgatagt tggggcgctg gcggaacgaa tccgcttctc agctgtgttg attttcgtgg
     4141 tggtatggct gacgctctct tacattccga ttgcgcatat ggtgtggggc ggcggtttgc
     4201 tggcttctca cggtgcactg gatttcgcgg gtggcacagt ggtgcacatt aacgccgcaa
     4261 tcgccggtct ggtgggcgcg tatctgatag gcaaacgcgt gggcttcggt aaagaggcgt
     4321 ttaaaccgca caacctgccg atggtcttca ccgggactgc cattctctat atcggttggt
     4381 ttggctttaa cgccgggtca gcgggcacgg cgaatgaaat cgcggcactg gcatttgtga
     4441 atactgtggt cgcaacggcg gcggcaattc ttggctggat cttcggtgaa tgggcgctgc
     4501 gtggtaagcc ttcactgctg ggggcgtgtt ctggcgcgat tgccggtctg gtcggcgtga
     4561 cgccagcgtg cggctacatt ggggttggcg gcgcgttgat tatcggcgtg gtagctggtc
     4621 tggcgggctt gtggggcgtt accatgctca aacgcttgct gcgggtggat gatccctgcg
     4681 atgtcttcgg tgtgcacggc gtttgtggca ttgtcggctg tatcatgacc gggatttttg
     4741 ccgccagctc gctgggcggc gtgggcttcg ctgaaggtgt gacgatgggt catcagttgc
     4801 tggtacagct ggaaagcatc gccattacga tcgtctggtc cggtgtggtg gcatttattg
     4861 gctacaaatt ggcggatctg acggttggtc tgcgtgtacc ggaagagcag gagctagaag
     4921 ggctggatgt caacagtcac ggcgagaatg cctataacgc gtaacaagca ctgcaaaaaa
     4981 cagccggacg gttttcacct ccggctattt ttttaattgt gattacgcat caccccttcc
     5041 tgaacggtcg aggcaaccag tacgccgtcc tgggtataaa actcaccgcg cacaaagcca
     5101 cgtgcgctgg acgccgaggt gctctccacg ctatacagca gccattcatt caaattaaac
     5161 gggcgatgga accacatgga atggtcaatg gtggcaatct gaatccccgg ttcgagaaaa
     5221 ccgatgccgt gcggctgtag agctaccggc aggaagttaa gatcagaagc gtaaccgagc
     5281 agatactgat gaacgcgcag gtcatccggc acgctaccat ttgcgcggat ccacacctga
     5341 cgatgtggtt ctgcgacgtg acctttcagt gggttatgaa actccaccgg acggacttcc
     5401 agcggacgat cgcagatgaa tttatcttta agcactggcg gcagcaggtg cgccagcgat
     5461 tgggcgattt gcgtttccga aggcaggcca tcaggcgctg gcgcggacgg cattgttttt
     5521 tgatgttcga aacccgcttc tggtgcctgg aaagaggccg tcatataaaa aatcggtttg
     5581 ccgttttgaa tagcagcaac ccggcgggcg ctgaagctgt taccatcacg cagcgtttcg
     5641 acatcataaa taatcggttt cttactatcg cctgggcgaa gaaagtagct gtgaaacgaa
     5701 tgtaccagcc gctcttccgg gacggtctct tttgcagcat acaaggcctg acccacgacc
     5761 tggccgccaa acacctggcg taaacctaaa tcttcactct ggccgcgaaa gagtcctgcc
     5821 tcaatttttt ccagatttaa caatgccagt aaatttttta gcgcctgact catataactc
     5881 tccagtaaca aagctgccgc agcaagccaa agtgagttga gtataacgca aatttgccac
     5941 tggtccgatg ggtgcaataa tctgaattac gggctaatta caggcagaaa tgcgtgatgt
     6001 gtgccacact tattgacgtt actattttgt taaccactct tccggcgagg aaagttagcc
     6061 cgctggtgca ttgataataa ggagaaatga atgaaactcg tgcacatggc cagtggttta
     6121 gcggttgcga ttgcgttggc ggcttgcgca gataaaagcg cggatattca gacgccagcc
     6181 ccggctgcaa atacgtctat ttcagcaaca caacaacctg ctatccagca accgaatgtc
     6241 tccggtaccg tctggatccg gcagaaagtc gcactgccgc ctgatgctgt gctgaccgtg
     6301 acactttctg acgcgtcgtt agccgatgca ccgtcaaaag tgctggcgca gaaagcggtg
     6361 cgtaccgaag gtaaacagtc accattcagc tttgttctgc catttaaccc ggcagatgtt
     6421 cagccgaacg cgcgtattct gttgagtgcg gcgattaccg tgaatgacaa actggtattt
     6481 attaccgata ccgttcagcc ggtgatcaac aagggcggaa ctaaagccga cctgacattg
     6541 gtgccggtgc agcaaaccgc cgtgccggtt caggccagcg gtggtgcaac gactaccgta
     6601 ccttcgactt caccaactca ggtgaatccg tcttcggcag ttccagctcc tacgcaatat
     6661 taagcgacgt taaccctctc cgcccggaga gggttagtag ttccagcgat aacgctgcaa
     6721 gtcgatttgc ccgcttcccg ataccatcac accttctgcc agtaatgcct gtcgctgacg
     6781 ctgtaaatcc ggtccggtta gcgaaattgt gccgtggcga ttaaccaccc ggtgccaggg
     6841 taaggtgctg ccttcaggga gacgctttaa cacaccgccc acctggcgcg cggcgcgggg
     6901 cgatcccgcc agtttcgcca catcaccgta agtggtgaca tagccttcgg gaatagcggc
     6961 gacgatttgc cagacgcgtt ggggaaatga atcttctttt tccatctttt cttcctgagg
     7021 taatttttca gcataatctg gaaaaacgcc cgagtgaagt cgcattgcgc aagaaaccag
     7081 catctggcac gcgatgggtt gcaattagcc ggggcagcag tgataatgtg cctgcgcgtt
     7141 ggttctcaac gctctcaatg ggggctctgt tggttctccc gcaacgctac tctgtttacc
     7201 aggtcaggtc cggaaggaag cagccaaggc agatgacgcg tgtgccggga tgtagctggc
     7261 agggccccca cccatttctg cctcccaccg tttcgtcaaa aaaaaccaac atggctaaac
     7321 tttaaccaca actgacgtcg caagaattgt ctggctgcgc agtacgcttc ggaggtatgt
     7381 ctgatgaagt atgttgatgg ttttgtggtt gccgttcctg ccgataaaaa ggatgcctat
     7441 cgggaaatgg ccgctaaggc tgcgccattg tttaaagagt ttggcgcgct tcgtattgtc
     7501 gaatgctggg ccagcgatgt accggatggc aaagtgaccg attttcgtat ggcggtgaaa
     7561 gcggaagaga atgaagaggt tgtctttagc tggattgaat acccttcaaa agaggtccgc
     7621 gacgctgcta atcaaaagat gatgtcggac ccacggatga aagagttcgg cgagtccatg
     7681 ccgtttgacg gcaagcgaat gatctatggc ggattcgagt caatcatcga cgaataggta
     7741 gcgtgacggg ctgtgcggcg tgccgtcagc cc
//
LOCUS       DAJVUJ010000179         7660 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.179, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000179 DAJVUJ010000000
VERSION     DAJVUJ010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7660
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.179"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            380..988
                     /locus_tag="OQQ48_003658"
     CDS             380..988
                     /locus_tag="OQQ48_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HCS1954280.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            1086..2477
                     /gene="selA"
                     /locus_tag="OQQ48_003659"
     CDS             1086..2477
                     /gene="selA"
                     /locus_tag="OQQ48_003659"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HCS1954281.1"
                     /translation="MTTETRSLYSQLPAIDRLLHDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRDSQTLPAWCENWAQEVDARLTKEAQSALRPVLNLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2474..4318
                     /gene="selB"
                     /locus_tag="OQQ48_003660"
     CDS             2474..4318
                     /gene="selB"
                     /locus_tag="OQQ48_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HCS1954282.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPSFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPGREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADAPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFSDEPWW
                     VRDLAKETGTDEQAMRLTLSQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
     gene            4508..5659
                     /gene="yiaY"
                     /locus_tag="OQQ48_003661"
     CDS             4508..5659
                     /gene="yiaY"
                     /locus_tag="OQQ48_003661"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HCS1954283.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDSMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAYQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNSLKDACGFTNPIQATHEEIVAIYRAAM"
     gene            5824..7362
                     /gene="aldB"
                     /locus_tag="OQQ48_003662"
     CDS             5824..7362
                     /gene="aldB"
                     /locus_tag="OQQ48_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HCS1954284.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
BASE COUNT         1812 a         1919 c         2137 g         1792 t
ORIGIN      
        1 ggattgaccg gatggccgga cgtcactccg ccggggcaca ccgaacaggc cacgccggtg
       61 ggggtaccaa tgcgcacccc ggctgaaccc tgaacaatgc taccgccata ctgcgtcatg
      121 tcgccctgac gcgctgccgg ttttccgctc atttctcatc cttgttttat cgggttattt
      181 gtctgctgac aggcaaaacc ctacagattt ataagaaagc ctgaagtgag atatccatca
      241 gagtgaaata attaggaaaa tatttatatc accatgataa atatgtgatt tatttggaag
      301 taacaaacca tcaacatttt ctgccctttt tcccatcacc aggccatact gatatttttc
      361 ctcgcacaaa ggactcggca tgaaactcgt cggtagctac accagcccgt ttgtacgcaa
      421 actttctatt ctgttgttag aaaagggcat aactttcgaa tttattaatg aactgcccta
      481 taacgcggac aacggcgtgg cgcaatttaa cccgttagga aaagtgccgg tgctggtgac
      541 cgaagagggc gaatgctggt ttgattcgcc gatcatcgct gaatatattg agttaatgaa
      601 tgtcgctccg gcgatgttgc cgcgcgatcc gctggagtcg ttgcgggtgc gcaaaattga
      661 ggcgctggcg gatggcatta tggatgccgg gctggtatcg gtgcgtgaac aggcgcgtcc
      721 ggcggcgcag cagtctgaag atgaattgtt acgccagcgg gagaaaatca accgcagtct
      781 ggatgtgctg gaaggatatc tggtcgatgg cacactcaaa accgatacgg tcaatctggc
      841 gactatcgcc attgcctgtg ctgtcggata tctcaatttc cgccgcgttg cgccaggttg
      901 gtgtgtcgac cgcccacact tagtcaaact ggtcgaaaac ctgtttagcc gcgaaagttt
      961 tgctcgcacc gaaccgccaa aggcttgatg cgcgatatgt cctcctgacc catctcacgt
     1021 tacaatccgt ggttatgtta aacgcccttc tccatgtgag agggccttga ttagccaggt
     1081 ttcctatgac aaccgaaacg cgttccctct atagtcaact tccggctatt gatcgcttat
     1141 tgcacgatag ctccttcctt tctttgcgtg atacttatgg tcacacccgc gtggtggaat
     1201 tgttgcgtca gatgctcgac gaagcgcgag aagtgattcg tgacagccag acgcttcctg
     1261 cgtggtgtga aaactgggcg caagaagtcg atgcccggtt gacgaaagag gcgcagagcg
     1321 cgctgcgtcc ggtgctcaac ctgacgggaa ccgtgctgca taccaacctt ggacgagctt
     1381 tacaggcgga agccgcggtg gaagccgttg cgcaggctat gcgttcgcca gtgaccctcg
     1441 agtacgatct ggacgacgct ggacgcggac atcgcgatcg ggcgctggcg caactgctgt
     1501 gccgtattac gggggcggaa gatgcctgta tcgtcaataa caatgcggcg gcggtgttat
     1561 tgatgttggc ggccactgcc agcgggaaag aggtggtagt atctcgcggc gaattggtgg
     1621 agattggcgg cgcgtttcgt attcccgatg ttatgcgtca ggcaggctgc accctacacg
     1681 aagtagggac caccaaccgc acgcacgcga atgattatcg tcaggcggtg aatgaaaata
     1741 ccgcactgtt gatgaaagta cataccagta actacagcat tcaggggttc accaaagcga
     1801 tagatgaagc ggaactggtg gcgctcggca aagagctgga tgttcccgta gtgactgatt
     1861 taggcagtgg ctcgctggtc gatcttagcc agtacggttt gccgaaagag ccaatgccgc
     1921 aggagttgat tgcggcgggc gtcagtctgg tgagcttctc cggcgacaag ttgttaggcg
     1981 ggccgcaggc agggattatc gttggtaaaa aagagatgat cgcccgcctg caaagccacc
     2041 cgctgaagcg tgcattacgc gcggataaaa tgaccctcgc ggcgctggaa gccacgttgc
     2101 gtctttattt acaccctgaa gctctgagtg aaaaattacc gaccctgcgc ctgcttaccc
     2161 gcagcgcaga ggtcattcaa atccaggcac aacgtttaca ggctcccctt gccgcacatt
     2221 acggcgcgga gtttgcggta caggttatgc catgtctttc gcagattggc agtggttcgc
     2281 tgccggttga tcgcctgccg agcgcggcat taacgtttac accccatgat ggacgcggta
     2341 gccaccttga gtcattagcc gcccgctggc gtgaattgcc agtgccggtg attggtcgta
     2401 tttatgacgg acgattgtgg ctggatttac gctgccttga agatgagcaa cggtttttgg
     2461 agatgttgtt gaaatgatta ttgcgactgc cggacacgtt gaccacggca aaacaacctt
     2521 attgcaggcg attactggcg taaatgctga ccgtctgccg gaagaaaaaa agcgcggcat
     2581 gaccatcgat ctcggctatg cctactggcc gcagccggat ggtcgcgtgc ctagttttat
     2641 cgacgtaccc ggtcatgaaa agtttctttc caacatgctg gcgggcgttg gtggtatcga
     2701 tcacgcgctg ttggtggtgg cgtgcgatga cggcgtgatg gcgcagaccc gtgagcatct
     2761 ggcgattttg cagctgaccg gtaacccgat gctgacagtg gcgctgacca aggccgatcg
     2821 cgtggacgaa gcgcgtgttg atgaggttga acgccaggta aaggaggtgc tgcgggaata
     2881 cggttttgct gaggcaaaac tgtttatcac cgcagcaacc gaaggtcggg gaattgatgc
     2941 cctgcgcgag catctgcttc agttaccagg acgtgaacac gccagccaac atagtttccg
     3001 cctcgcgatc gaccgcgcat ttaccgtaaa aggtgccggg ctggtcgtca ccggtacggc
     3061 gttaagcggg gaagtgaagg taggcgattc actctggctg actggtgtaa ataaaccgat
     3121 gcgtgtacgt gcgctgcatg cacaaaacca gccaacagaa accgcccatg ccgggcagcg
     3181 tatcgcgctt aacatcgcgg gtgatgcgga aaaagagcag attaaccgtg gcgactggct
     3241 gcttgccgat gcgccgccag agccgttcac acgggtgatt gtcgagcttc aaacccatac
     3301 accgctgacc cagtggcagc cgctgcatat tcaccacgcc gccagccacg tcacgggacg
     3361 cgtttcactg ctggaagata accttgctga actggtcttc gacaccccgt tatggctggc
     3421 agataacgac cgcctggtat tgcgcgatat ctctgcccgc aacacgctgg ccggagcgcg
     3481 cgtcgtgatg cttaacccgc cgcgtcgcgg taaacgtaaa ccagagtatc tgcaatggct
     3541 ggcttccctc gccagggcac agaatgatag cgaagcgtta gccgttcacc tgcaacgcgg
     3601 cgcggttaat cttcctgatt tcgcctgggc gcgccagctc aacggcgaag ggatgcgcga
     3661 actgctgcaa cagcctggct atattcaggc tggttatagc ttgttgaatg cgccggttgc
     3721 cgctcgctgg caacggaaaa ttctcgacac attagcgact tatcatgagc aacatcgcga
     3781 tgaacctggg cctgggcgcg aacgtctgcg acgtatggcg ttgccaatgg aagatgaagc
     3841 gctagtactg ttgctgattg aaaagatgcg cgaaagcggc gacatcctca gtcatcacgg
     3901 ttggctgcat ctgccggatc acaaagcggg cttcagcgaa gagcagcagg ccatctggca
     3961 aaaagcagag ccgctgttta gtgacgagcc gtggtgggtg cgtgatctgg caaaagagac
     4021 gggtaccgac gagcaggcaa tgcgcttgac gctaagccag gcggcgcagc aaggaataat
     4081 taccgcgatc gtcaaagatc gttattaccg taacgatcgg attgtcgaat ttgccaatat
     4141 gatccgcgat ctcgatcagg agtgtggttc aacctgcgcg gcggatttcc gcgatcgctt
     4201 aggcgtaggc cgaaagctgg caattcagat tctggaatat tttgaccgca ttggctttac
     4261 gcgtcgtcgt ggaaatgatc atttattgcg cgacgcatta ttatttccgg aaaaataagg
     4321 aaatgattaa ttaattttta aaataaatta atacaaattt cttatgattt taaaaaaagc
     4381 acattgttta atgaatacaa tgtgcttttt attagattaa ttttgataat taataataat
     4441 gatcatgttt tcgaatgaaa atcctcagta agcttcccac ccttttttac actttcagga
     4501 gtgtgttatg gcatcttcaa ctttctttat tccttctgtg aatgtcatcg gcgctgattc
     4561 attgactgat gcaatgaata tgatggcaga ttatggattt acccgtacct taattgtcac
     4621 tgacagtatg ttaacgaaat taggtatggc gggcgatgtg caaaaagcac tggaagaacg
     4681 caatattttt agcgttattt atgatggcac ccaacctaac cccaccacgg aaaacgtcgc
     4741 cgcaggtttg aaattactta aagagaataa ttgcgatagc gtgatctcct taggcggtgg
     4801 ttctccacac gactgcgcaa aaggtattgc gctggtggca gccaatggcg gcgatattcg
     4861 cgattacgaa ggcgttgacc gctctgcaaa accgcagctg ccgatgatcg ccatcaacac
     4921 cactgcgggt acggcctctg aaatgacccg tttctgcatc atcactgacg aagcgcgtca
     4981 tatcaaaatg gcgattgttg ataaacatgt cactccgctg ctttctgtca atgactcctc
     5041 tctgatgatt ggtatgccga agtcactgac cgccgcaacg ggtatggatg ccttaacgca
     5101 cgctatcgaa gcatatgttt ctattgccgc cacgccgatc actgacgctt gtgcactgaa
     5161 agccgtgacc atgattgccg aaaacctgcc gttagccgtt gaagatggca gtaatgcgaa
     5221 agcgcgtgaa gcaatggctt atgcccagtt ccttgccggt atggcgttca ataatgcttc
     5281 tctgggttat gttcatgcga tggcgtacca gctgggcggt ttctacaacc tgccacacgg
     5341 tgtatgtaac gccgttctgc tgccccatgt tcaggtattc aacagcaaag tcgccgctgc
     5401 acgtctgcgt gactgtgccg ctgcaatggg cgtgaacgtg acaggtaaaa acgacgcgga
     5461 aggtgctgaa gcctgcatta acgccatccg tgaactggcg aagaaagtgg atatcccggc
     5521 aggcctacgc gacctgaacg tgaaagaaga agatttcgcg gttctggcga ctaattccct
     5581 gaaagatgcc tgtggtttta ctaacccgat ccaggcaact cacgaagaaa ttgtggcgat
     5641 ttatcgcgca gcgatgtaat catcatttcc acaacggctg gcaaattgtc agccgctttt
     5701 tcaactatct ctgtaaccct tgcccgtaaa ttcgtgatag ccgtcgtaaa gctgttaccg
     5761 actggcgaag atttcgccag tcacgtctac ccttgttata cctcacaccg caaggagacg
     5821 atcatgacca ataatccccc ttcagcacag attaagcccg gcgagtatgg tttccccctc
     5881 aagttaaaag cccgctatga caactttatt ggcggcgaat gggtagcccc tgccgatggt
     5941 gagtattacc agaacctgac gccggtgact gggcagctgc tgtgcgaagt ggcgtcttcg
     6001 ggcaaacgag acatcgatct ggcgctagat gctgcgcaca aagtgaaaga taaatgggcg
     6061 cacacctcgg tgcaggatcg cgcggcgatt ctgtttaaga ttgccgatcg aatggaacaa
     6121 aacctcgagc tgttagcgac agctgaaacc tgggataacg gcaaacccat tcgcgaaacc
     6181 agtgctgccg acgtgccgct ggcgattgat cattttcgct atttcgcctc gtgtattcgg
     6241 gcgcaggaag gtgggatcag tgaagttgat agcgaaaccg tggcctatca tttccatgaa
     6301 ccgttaggcg tggtggggca gattatcccg tggaacttcc cgctgctgat ggcgagctgg
     6361 aaaatggctc ccgcgctggc ggcgggcaac tgtgtggtgc tgaaacccgc acgtcttacc
     6421 ccgctttctg tactgctgct aatggaaatt gtcggtgatt tactgccgcc gggcgtggtg
     6481 aacgtggtca atggcgcagg tggggaaatt ggcgaatatc tggcgacctc gaaacgcatc
     6541 gccaaagtgg cgtttaccgg ctcaacggaa gtgggccaac aaattatgca atacgcaacg
     6601 caaaacatta ttccggtgac gctggagttg ggcggtaagt cgccaaatat cttctttgct
     6661 gatgtgatgg atgaagaaga tgcctttttc gataaagcgc tggaaggctt tgcactgttt
     6721 gcctttaacc agggcgaagt ttgcacctgt ccgagtcgtg ctttagtgca ggaatctatc
     6781 tacgaacgct ttatggaacg cgccatccgc cgtgtcgaaa gcattcgtag cggtaacccg
     6841 ctcgacagcg tgacgcaaat gggcgcgcag gtttctcacg ggcaactgga aaccatcctc
     6901 aactacattg atatcggtaa aaaagagggc gctgacgtgc tcacaggcgg gcggcgcaag
     6961 ctgctggaag gtgaactgaa agacggctac tacctcgaac cgacgattct gtttggtcag
     7021 aacaatatgc gggtgttcca ggaggagatt tttggcccgg tgctggcggt gaccaccttc
     7081 aaaacgatgg aagaagcgct ggagctggcg aacgatacgc aatatggcct gggcgcgggc
     7141 gtctggagcc gcaacggtaa tctggcctat aagatggggc gcggcataca ggctgggcgc
     7201 gtgtggacca actgttatca cgcttacccg gcacatgcgg cgtttggtgg ctacaaacaa
     7261 tcaggtatcg gtcgcgaaac ccacaagatg atgctggagc attaccagca aaccaagtgc
     7321 ctgctggtga gctactcgga taaaccgttg gggctgttct gatataagaa gctggtcgca
     7381 ttgggtattc attgcctgat gtgacgctta cgcgtcttat catgcctacg ggaacctgac
     7441 cgtaggccgg ataaggcgta cacgccgcat ccggcataaa caaagcgcac gttgttacaa
     7501 tttacgtttt aaacctacgt gcgcaataat ccagactacg ccaacagaag tgcgtttctg
     7561 gggcgtgttt tgtcaggagt ttaatctcgc gtttcgatgg tgagcgggcg aactaggccg
     7621 taaatcacca ttggtgatcc gaggccaatc agcaataaac
//
LOCUS       DAJVUJ010000180         7527 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.180, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000180 DAJVUJ010000000
VERSION     DAJVUJ010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7527
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.180"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..590
                     /locus_tag="OQQ48_003663"
     CDS             <1..590
                     /locus_tag="OQQ48_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCS1954285.1"
                     /translation="INQLADKQTIKQTLFARQPAKIHKPYPTNSKIIMGDVLNHAALK
                     QAMQGQDVVYANLTGEDLDIQANSVIAAMKACDVKRLIFVLSLGIYDEVPGKFGEWNN
                     AVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYELTSRNEPFKGTIVSRKS
                     VAALITDIIDKPEKHIGENIGINQPVTDGDKPLFM"
     gene            900..1628
                     /gene="mpaA"
                     /locus_tag="OQQ48_003664"
     CDS             900..1628
                     /gene="mpaA"
                     /locus_tag="OQQ48_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HCS1954286.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(1603..2568)
                     /gene="ycjG"
                     /locus_tag="OQQ48_003665"
                     /pseudo
     CDS             complement(1603..2568)
                     /gene="ycjG"
                     /locus_tag="OQQ48_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
     gene            2687..3193
                     /gene="tpx"
                     /locus_tag="OQQ48_003666"
     CDS             2687..3193
                     /gene="tpx"
                     /locus_tag="OQQ48_003666"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HCS1954287.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAFGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(3237..4778)
                     /gene="tyrR"
                     /locus_tag="OQQ48_003667"
     CDS             complement(3237..4778)
                     /gene="tyrR"
                     /locus_tag="OQQ48_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HCS1954288.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(4926..5987)
                     /locus_tag="OQQ48_003668"
     CDS             complement(4926..5987)
                     /locus_tag="OQQ48_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HCS1954289.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQHFAPATLDE
                     APEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREICRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(5984..7381)
                     /locus_tag="OQQ48_003669"
     CDS             complement(5984..7381)
                     /locus_tag="OQQ48_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HCS1954290.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSVKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDTWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
BASE COUNT         1860 a         2078 c         1813 g         1776 t
ORIGIN      
        1 tgattaatca actggcagat aaacaaacca ttaaacaaac tttatttgcc cgtcagccag
       61 caaaaatcca caaaccctat ccaactaata gcaagattat tatgggagat gtactgaatc
      121 atgcagcact gaagcaggcc atgcagggac aggacgttgt gtatgccaac cttacggggg
      181 aagatttaga tattcaggca aattcagtga tcgctgcgat gaaagcctgt gatgttaaac
      241 gtctgatctt tgttctttcc ctcggtattt atgatgaagt tccaggaaaa tttggcgaat
      301 ggaataatgc agttattggt gagcccctaa aaccattccg cagggctgcg gatgcaattg
      361 aggcttctgg actggaatat acaattcttc gccctgcctg gctgactgat gaagatatta
      421 ttgattatga gcttacctca cgcaatgaac cattcaaagg tacgatcgta tcacgtaaga
      481 gtgtcgcagc attaatcaca gatatcatcg ataaacctga gaaacacatt ggtgagaata
      541 taggtatcaa tcaacccgtg actgatggtg acaagccatt attcatgtaa atcaattaca
      601 tagttaccat cgtaatgtac tatgtgcagt acgatttatt aaacttatta aggtgaactg
      661 atcaatccga tgcgattaac atgtgtagaa ccagtaagaa taaaatacgc catgtatata
      721 acattacgac ttacatctaa gtcagcataa ttctaatatc tgcacatcaa taaaaatgca
      781 aacaacttcc ggcaaaaaaa gtgatacaag atcgatgaca tagcgttgaa gaaaaggatt
      841 tgataatccg caaaatttgc acaacactgc ctgtaatact tcctgaacca gagacgatca
      901 tgaccgtaac ccgcccacgc gccgaacgcg gcgcatttcc gcccggaaca gaacattacg
      961 gacgttcatt attgggcgcg ccgttaatct ggtttcctgc ccctgcagcc agccgcgaaa
     1021 gtggtttgat tctggctggc actcacggtg atgaaaactc ttcggtcgtc acgctctcct
     1081 gtgctctgcg gacgttgaca ccttctttgc gccgccatca tgtggtgctg tgtgtgaatc
     1141 ctgacggctg ccagctgggg ttacgggcca atgctaatgg tgtggattta aacagaaact
     1201 ttccggcggc gaactggaag gaaggtgaaa cggtttatcg ctggaacagc gccgctgaag
     1261 aacgtgatgt cgtattactg acaggtgaca aacctggctc agaacctgaa acccaggcgc
     1321 tgtgccagct tatacaccgc attcaacctg cctgggtggt ctcattccac gatccactgg
     1381 cctgtattga agatcccaga cacagcgaat taggcgaatg gctggcccag gcgtttgaat
     1441 tgcctctggt aaccagcgtc ggttatgaaa cgcctggctc tttcggcagt tggtgtgcag
     1501 atctcaactt gcattgcatc accgcagagt ttccgcccat ctcctccgac gaagccagcg
     1561 aaaaatatct ctttgcgatg gcaaacttgc tgcgctggca tcctaaagat gcaattcgcc
     1621 cgtcgtgaac tgaagcgccg gttccacatc taccgccagc caggtcggtc cgtcaagatc
     1681 ggcgaaactg acctgcggca ccagcggtaa agcggcgcta atggcacgag aggtacacaa
     1741 catgcagccc agcatcagac tgaaaccttg tgcacgcgct tcagtcgcca gcgccagcgc
     1801 ttccgtcaga ccaccggttt tatcgagctt aatgttaacc atctcatagc gccctttcag
     1861 cgccttcaga ttgctacgtg tatgacaact ctcatcagca caaatcggca acggatgaat
     1921 aaaattctcc agcgccgcat cgtcctgcgc aggaaggggt tgttcaagca tcgcaacgcc
     1981 taaatccgcc aatagctggc aacgcgccgc caacccttct gcacgccagg attcatttgc
     2041 atcaacgatc agcgtcgcat cgggcacagc tgtgcgaatt gccaccatcc gctcactaat
     2101 aagatggtta tccagcttca ctttcagtaa tttcgcgcct gcctgccaga gtgttgatgc
     2161 actattggcc atctgatcag gcgtaccgat gactaccgtc tgtgcagtga taactgtctc
     2221 gggaagcgtt atgccgatca aatcagccag cgattgctgc tgttttcgcg cggccagatc
     2281 ccacaatgca caatccagcg cattacgtgc tgcgccggca gggagaattt tttgcaactc
     2341 ctcccgtgtc agccctttct ctaattgcga cacgacgctc ataatttgcg ccattaccga
     2401 ggcatcactt tctccataac gcggatacgg cgtgcattcg ccggtgcctt taataccctc
     2461 ttcttccagt tcaaccacca ccacgcgcgc ttcactgcga cttccccggg caatcacaaa
     2521 cggggtatgt aagggctagg cttcctcaaa taccttaacg gttctcatct ttactccttg
     2581 ttgtcgcggt ggtctgcgga ttaagcgcaa atagcgtttg ctgtgttatt gacagttagc
     2641 ataaactagg tgtgacgtta actatatgta aacaggaaag ataatcatgt cacaaaccgt
     2701 tcatttccag ggcaacccgg ttacagtcgc caattccatc ccgcaggcgg gtagcaaagc
     2761 gcagactttt actctcgtgg caaaagatct gtctgacgtc accctcggtc agtttgcggg
     2821 taaacgcaaa gtgctgaaca ttttcccgag tattgatacc ggtgtttgcg ccgcatcagt
     2881 acgtaagttt aaccaactgg caaccgagat cgacaacacc gttgtgctgt gtatttctgc
     2941 cgatctgccg ttcgcccagt ctcgtttctg cggcgcagaa ggtctgaaca acgttatcac
     3001 cctctccact ttccgtaacg ctgaatttct gcaagccttc ggtgtggcaa ttgctgatgg
     3061 cccactgaaa ggtctggcag cgcgtgccgt tgtggttatt gacgaaaatg acaatgtgat
     3121 tttcagccag ctggtggatg aaatcaccac cgagccggat tacgaagcag ctctggctgt
     3181 actgaaagca taagttaata tgcctgatgg tgttgcacca tcaggcatat tcgcgcttac
     3241 tcttcgttct tcttctggct taagccatat tcccgcaact tattggcaat cgcggtatgt
     3301 gaaacgccga gacgttttgc cagtttgcgc gtgctgggat aattgcgata aagctgggtt
     3361 aataccgagc gttcaaaacg gctggtgatt tcgtccagcg aaccttccat cgcatcttcg
     3421 cccacggcta ccgttgcggc gtcataatcc ggcaacaaaa tatcctgtgg acgcagctca
     3481 taaccgtcca gttgtgtcag tgcgcgatat atagcgttct ttaactgccg cacatttccc
     3541 ggccacgcat aacgcgtaag tacagtattc aggtcagcgg ccagtttcgg acgcggcacg
     3601 ccctgctcgt cggcaaagcg ggcgacgaac agctcagtta acggcatgat gtcctgcgga
     3661 cagtcacgta gcggcggcag attgagcgtc aacacgttca gacgataata gagatcttca
     3721 cggaacatgc ctttttgcac cagttcgacc agattcttct gcgtagcgca aatcacccgc
     3781 acatcgacat gcacctcatg gtcttcgcca acccgacgga aagtgccatc attaagaaaa
     3841 cgcagtaatt tcgcctgcat ccgtggtgac atttccccta tttcatccaa cagcaccgaa
     3901 ccaccgttcg cctgctcaaa gaatcctttc ttcccttccg gagcatgacc aaacagttca
     3961 ctctcgaccg catcttccgg tatagacgca cagttcagcg ccaggtaagg tttgcccgct
     4021 ctggggcttg cctgatggca ggcgtaggca aagagatctt tacctgtacc tgtgtcaccc
     4081 gtaatcagca gcggcgcgct tagcatcgcc agtttctgcg cctgttcgac aacatgcttc
     4141 attttcgggc tgacggcgac aatttgactg aaggcgctga cgtcctgggc ggcgacattt
     4201 tgcaactggc ggcccatacg aatcgttgat cgcaacatca ccaccgcacc ggtcaggacg
     4261 tgttgatcat tttcatcctg aagataaaca ggcgtaatct ccatcaggaa attctgccca
     4321 ttaataacga catgctcgtt atgcgaatct tgcggttcgc tttccagcca acgtaaaaaa
     4381 ttaaagccgt taatcaattg tgcggcggta tggttgcgca ggcgatccaa tttttgccca
     4441 aaaagctgac agctcgccgg gttcgccata tccactttgc ttttcatatc gacagagagc
     4501 acaggttcgg gcaacgcctc cagtaacgcg ctcaacgcca gatgctcacg ttcggaaggc
     4561 atccacggga cagtacgcac atcggtaaca cctgcaatac ggcgtatttc ggccatcaga
     4621 ctgctgaaac tctcaaactc cagttcagca aaattgaggt agattcgccc aatgggatca
     4681 atctcaatac cgcgtaaatc aatgcctctt agcacgagta gatcgagtaa ttcgcgggtc
     4741 agaccgagtc ggtcttcaca aaagacttcc agacgcatgg gaaccttcac ctgaaaaaag
     4801 aacaattaat atgatgatat gacactattt ggatagcagg aaggtttctg tcaacaatca
     4861 ttgacacaaa gacggaaaaa cgggcacttc ggcgtaaaga tatccggtca gcacccgata
     4921 ttgcattatt tttcgctggg cgttttgcct ttttgcagcg tttctttcac ctgaccgata
     4981 agctgacgac ggaaatcccc gaggcgaggt ttgtcatcgt caatccacgg cagcgggcgg
     5041 caaagctcca tagctttaat cccgagtcgt gccgtcagaa gtccagcacc aattccctga
     5101 gctgcgcggg tagacaaacg agcagcgaga tcttgcgaca tccagtccat ccccacttcg
     5161 cgcaccagtt cgctggctcc ggcaaaagcg atattcagca ataccagctt aaacagacgc
     5221 aaacggctgt aataccccag ttcaatgcca tacagcgtgg cgatgcgatt aattaaacgc
     5281 agattgcgcc aggcgataaa cgccatatcg accaacgcca gcgggctgac cgcaatcatc
     5341 aacgttgatt ccgccgccga acggcagatt tcgcgccgcg cctgggcatc taaaactggc
     5401 tggaccaaat gcgcatacaa actgaccact tcacggtcgt tttgcgtttc atggattgag
     5461 gcataccagc gttgcagcgc tggatgagac tgatcaatac ccgcctgctg cgccagtttt
     5521 tcgcaaaatg cgcggccttt gcccgtgccg tggctgtgca acaaatcgcg cgcttcgtcg
     5581 cgttcatggg cgcgctgtcg caagcgccat aagcgccgcc actctgttac cacagaacct
     5641 acgccagcgc cgataatcaa tgccccagcg gcacatccac ccagcgccac ccagtcctga
     5701 gtttgccagg cattcattgt ccactgtaca ccctgcccga caacgcttgc gccaaacaga
     5761 gccagcccgc ccatcaccat tttgcgccac aggctgcgtt tcggacgtaa cgctgcatcc
     5821 attaccgctt caacttgccc ctcttcttca ggcgcttcat cgagcgtggc cggggcaaaa
     5881 tgttgcgcct gattttcgtc aaaggtctgc tgcgccctga attttggatt ctgatcgacc
     5941 tccagcggac cgtcgaaatc aatacgtggt tttaacggtt cggtcatcgc aatttatctc
     6001 ctattaaaaa ttccagcgcg gcatcaagac gaatatgcgg cagcggtttg tcgacatcca
     6061 tcacctgcgg gcgaaacgct tcaaactgga acccttgttt gtcccagaac gcctgaccgg
     6121 gcaaacgcgc aggaacttca ccgggataaa cggtgagtgg cgcaccatcg ctgagccggt
     6181 taccgcgcaa tgccggaatt ttctcgccgt tgacgtcaat aatgccgctg gtggtcgcct
     6241 gcaccgaggc cagccccagg caatccatac taatcccttc aaacgccgca ttttgccagg
     6301 catcctgaat cagttgttgc agtaatgaaa ccatattggc gtgctgatcg atggtcacat
     6361 ggtccgcttt agtggcagca aacaatagct tatcgataac cggcgaaaac aaacgccgga
     6421 acaaggtacg ctgcccgtag tggaaacttt gcatcagctg cgtcagtgcc agacgcatat
     6481 cattaaatgc ctgtggccca ctgttgagag gttgcaggca atcaaccagc acaatctggc
     6541 ggtcaaagcg cagaaaatga ttcttataga accccttcac caccttctcg cagtaataat
     6601 taaaccgctc gcgcagcatt ccggcattgg tgtgcttatc ggcctgcgcc agtttggact
     6661 cgccccaggt atcgacatcc ggccacggga agaattgcag cgcgggcgca cctgccatat
     6721 ctcctggcaa gacaaagcgc cctggctgaa taaagtgcag cccctgctgt ttacagtggt
     6781 ggagataatc ggtccacgcg gcggcaatgt ctgccagccg gttttcgtcg gcaggtgcta
     6841 gcgggtccag cccttcgcac atcattcgcc atttgaccga ccattcgccg cgctgaccat
     6901 tgagtaagcc cgtcatctgg cgtgaccagc ttaaatagtc ctgcgccagc atcggcaggt
     6961 cgagcaacca ttcgccggga taatccacaa tctccagata cagcgtggag gtgtccttga
     7021 agtggcgtag cagcgaatcg ttcgatttaa aacgtagcgc caggcggatt tcactgacgc
     7081 cgcgcgttgg cgtcggccag gcgggaggat cgccatataa ctgcgccagt ccttcatcat
     7141 atgtgaagcg cggaatgcca aagtcacgct gaggaatgcg ttttacgccc agcaggcgct
     7201 cttcacgcac cgcgcttaac agcggcaagc gtgctccggc gtgaatattg agcaactgat
     7261 tgaccatagc agtgataaac gctgttttgc cgctgcggct tagtccggtt acggcgaggc
     7321 gcagatgtct gtcgacaccc cgattcacca gcgcattaag ttcattttta agtcgcttca
     7381 ttgctgtcct gtgctgctct gaagaccaga aaaaaatcat tcgtaaggat atgaatgtgg
     7441 gggccaggcc attgattatc aactgcccgt gttgtcatcg acgggcgaac tccataagat
     7501 gtgcagcatt cacgttcgga cggagtt
//
LOCUS       DAJVUJ010000181         7473 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.181, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000181 DAJVUJ010000000
VERSION     DAJVUJ010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7473
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.181"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..340
                     /locus_tag="OQQ48_003670"
     CDS             <1..340
                     /locus_tag="OQQ48_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCS1954291.1"
                     /translation="QTEAFLNAYGLSRFAPLGYDPRDLPIRDLAGYRKKGNHDGDPII
                     FYTFPAAFEQEIAKGFNTKQFAEVLKNAGMLTPPTSGRGYQGRVREDGRQIRVYVLNF
                     MAEESSQPEE"
     gene            715..1149
                     /locus_tag="OQQ48_003671"
     CDS             715..1149
                     /locus_tag="OQQ48_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCS1954292.1"
                     /translation="MTAQISAYGRLVADPQTRTTTNGNNMAMARLAVSLPCNAAEAGE
                     STFWLGVIAFGKQADALAKHQKGDLVSVAGNMQLNQWTGQDGGTQQGYQVVADSVISA
                     RTVRPGGKAGQRGQATDALRRAQQPSRDEYDQRPPFDDETPF"
     gene            1160..1801
                     /locus_tag="OQQ48_003672"
     CDS             1160..1801
                     /locus_tag="OQQ48_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002436679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1954293.1"
                     /translation="MSTQPIIIEFGSYRWPRHLAIHEADHAIAAWFFGIQEVEIALSG
                     NNRVAKTYLSGDVDECGAVAVWHANYPRNVAEVKSYTLSNDDTRLRLIDQVCRAMVIS
                     LAGIVAQSRYTGDDINALMETSGKADMKNVRDLSEVYRVAGGEDADIQRISLSRAEAL
                     IALKWWDVIALAKILENRNHMPASNFQRIMGDIDKPVPKPMTLAELDELVGEE"
     gene            1798..2148
                     /locus_tag="OQQ48_003673"
     CDS             1798..2148
                     /locus_tag="OQQ48_003673"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1954294.1"
                     /translation="MKPYILAAGLISSAPALSAIHCGGFTIQPDRYRTTINGDVVHIV
                     DAQFYGAPRDFSQAAIKLLPDAITTTDRQYRLETQGGNALLELMTRENPPRVLNRERC
                     DAGLTSFGWYTSAP"
     gene            2842..3453
                     /locus_tag="OQQ48_003674"
     CDS             2842..3453
                     /locus_tag="OQQ48_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1954295.1"
                     /translation="MRDRTRSYTTDLPRIGLPFLANMRSRLADAEPGTQLYTQTESGT
                     LYAFRQADSYAMTINGVTRAIRTTTTKAGYGVREWYACPHCMKRAAKLYIGKKDIGCR
                     ACWKLHYKSQSADILDRMRMKIRQQRCAIWGSNDLANNLFNDIRMFPKPKGMRWATFD
                     RKRAELPVMEMAYWQAFSPVVDRITGVIERKAGKAARGIGLTL"
     gene            3991..4533
                     /locus_tag="OQQ48_003675"
     CDS             3991..4533
                     /locus_tag="OQQ48_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="HCS1954296.1"
                     /translation="MYQLKITIRDSKPPIWRRVLVPEQIPFSELHAVIQLAFGWNDEH
                     LYMFEKGRKGDPGSEYRVWGEDESMGNAAITPLWAALQNDGDKLVYTYDFGDWWDCVI
                     VLEKQTHDTSNQPISCLRGKGTTPVENSGGLHGYNELLLQARESDNPEQAEIHNFLML
                     DIERRVYDLSSINDRLQTLN"
     gene            complement(4526..4885)
                     /locus_tag="OQQ48_003676"
     CDS             complement(4526..4885)
                     /locus_tag="OQQ48_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1954297.1"
                     /translation="MRMTSRKKEILSYFEPDNLEWVTGEIGAPPFDVSGVAYLLHGMV
                     SFDKRHQLESTRRTLESMVAGGLLERVTVYESRQIRRGGETNATVVRYGLPGQCAVMR
                     DTGGADNAISGEYMRVS"
     gene            5319..6143
                     /gene="dinD"
                     /locus_tag="OQQ48_003677"
     CDS             5319..6143
                     /gene="dinD"
                     /locus_tag="OQQ48_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HCS1954298.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKNQNILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGRKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
     gene            6435..7052
                     /locus_tag="OQQ48_003678"
     CDS             6435..7052
                     /locus_tag="OQQ48_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HCS1954299.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(7049..7473)
                     /gene="ligB"
                     /locus_tag="OQQ48_003679"
                     /gene_synonym="yicF"
                     /pseudo
     CDS             complement(7049..7473)
                     /gene="ligB"
                     /locus_tag="OQQ48_003679"
                     /gene_synonym="yicF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312549.2"
                     /note="this ligase is similar to LigA but it lacks the
                     C-terminal BRCT domain; catalyzes strand joining of nicked
                     DNA in the presence of a divalent cation and NAD+;
                     internal stop; incomplete; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
BASE COUNT         1997 a         1837 c         1909 g         1730 t
ORIGIN      
        1 gcagacggag gcgtttttga atgcctatgg gctgagtcga ttcgcgccgc tgggttatga
       61 cccgcgcgat ttacccatcc gtgatttagc cgggtacagg aagaagggga accacgacgg
      121 cgacccgata attttctaca ccttcccggc agcgtttgag caggagattg caaagggctt
      181 caataccaaa cagtttgccg aagttctaaa gaatgcagga atgcttacac caccaacgag
      241 cgggcgaggc tatcaggggc gagtaaggga ggatgggcga cagattcgcg tctatgtact
      301 caatttcatg gcagaggaaa gcagccagcc agaagaataa ttatttcttc acatgcgtag
      361 ttttagtgtt gtctcggttg tctttgttgt ctcaatttgt aattgagatt gatttataag
      421 gattttattt taatttctga gacaacaccg agacaacatt tagccatttt gagacaacag
      481 ggcgaaagtg tgtttttagt ctggcaggca gtgagacaac atttatgccg ggtgagacaa
      541 cacgaaattg caaatgttgt ctcagcaaaa cccagacgtg gcgcggcctg tagagccgtg
      601 agacaacaaa gacaacagag acaacactat ttttgcttac tatgaaaaaa aatcggcagg
      661 tgtcccacat taagaaatcg tcattagcaa aactaataat ccagtgaggg gaaaatgaca
      721 gcacaaattt cagcatacgg gcggctggtg gcagatcccc agacccgcac cacaacgaac
      781 ggtaacaaca tggctatggc gcggctggcg gtgtctctcc cctgtaatgc ggcagaggcg
      841 ggagagtcaa ccttctggct gggcgttatt gcttttggga agcaggccga tgcgctggca
      901 aagcaccaga aaggcgacct tgtgagcgtg gcaggcaata tgcaactcaa ccagtggaca
      961 gggcaggacg gcggtacaca gcaggggtat caggtagtag ccgacagtgt gatcagcgcc
     1021 agaacggtac gccccggcgg gaaggcagga cagcggggac aggcaacgga cgcgctgaga
     1081 cgtgcgcagc agccatcccg tgatgaatac gaccaacgcc caccattcga cgatgaaacg
     1141 ccattctgag agggaagata tgagtacgca acccatcatt attgagttcg gcagctatcg
     1201 ctggcccaga catcttgcaa tacatgaagc agatcatgcg atagccgcat ggtttttcgg
     1261 catacaggag gtagaaattg cgttatcggg taataaccga gtggcaaaaa cctaccttag
     1321 cggcgatgtt gatgaatgtg gagctgtcgc ggtatggcac gccaactatc cacggaatgt
     1381 ggctgaggtt aagtcttaca ccctctcgaa tgatgacacc aggttacgtc tgatagacca
     1441 ggtatgccgg gctatggtta tctctctggc tggtatcgtc gcgcaatcac gctatacggg
     1501 ggatgatatt aacgcactga tggaaacgtc cgggaaagca gacatgaaaa atgttcgtga
     1561 tttatcagag gtataccggg tggctggtgg ggaggatgct gatatacaga gaatatccct
     1621 ttcccgtgct gaggcgctga ttgccctgaa atggtgggat gttatcgcgc tggcgaaaat
     1681 actggagaac cgtaaccata tgcctgccag caacttccag cgcattatgg gtgatattga
     1741 caagcctgta ccaaagccta tgacgctggc tgaactggat gaattggtgg gtgaagaatg
     1801 aagccataca ttctggcagc cggcctgatc tcctcggcac cggcactatc tgctatccat
     1861 tgtgggggct ttaccataca gccagaccgt tatcggacaa cgattaacgg cgatgtggtt
     1921 catattgttg atgcgcaatt ttacggcgca ccgcgagact ttagccaggc cgccataaag
     1981 ttattgccgg atgccattac cactaccgat aggcaatacc gcctagagac gcagggaggc
     2041 aatgccttgc ttgagttgat gaccagagaa aacccgccac gtgtgcttaa tcgtgagcgc
     2101 tgcgatgccg ggttaacaag tttcggttgg tacacttctg cgccgtgatg ttcgttccga
     2161 gcgcagccta aaatattgga ctgttcttga cggatttaaa actctaatgg agccaagtcc
     2221 ttgccagtag cgggctagga gcctaattgt gattcaggtc gtcaaaaata ttcaggtttg
     2281 cggcattggt tcgcagtacg cagtccggta cgcggcaaaa ctgcgcattt aacgtgcgta
     2341 ccacctgacg aattctaacg ccggcattgc aaacagatgc gtgcgcaaaa tggctaagac
     2401 gtctgatatt gctggctttg gctcaatcag tttggaggtg gcggtatcag tacgatgcta
     2461 atttttgcta aggttttagt acgcaactca ggtacgcact ctcaccagat acgaaaccga
     2521 ttcgcagttg cgtaccagcc tgtaaggcgc attatcgctg ggtttctaca gtcgaatgca
     2581 gtacgcaaag tggaaaatcc tccgaatacg aaacccgagc attgcgcagt aaattgcgaa
     2641 ttgcgcagtt aacaaaatat ctccgcaaaa cgaaacggat atcaatgcgc aaaccatcct
     2701 caacccccgc gcggttactg gctttattga tgtgaaattg caaaattggt gcgcaattta
     2761 cagtaaaata cgcatattat ttcgtattca atttgcgcac cgttcgcagt taatgcgcag
     2821 taattcgcag gaggccagcc agtgagagac agaacgcgca gctataccac cgacctgccc
     2881 cgtatagggt tgccattcct tgcgaacatg cgtagcaggc tggcggatgc tgaaccgggt
     2941 acgcagcttt atacccagac cgaaagcgga acgttgtatg ccttccggca agccgacagc
     3001 tacgccatga caatcaacgg cgtgacgcgg gctattcgca ccacaaccac gaaggccggg
     3061 tatggcgtcc gggaatggta cgcttgcccc cactgtatga agcgggctgc aaagctgtat
     3121 atcggcaaga aagacattgg ctgccgggcg tgctggaaac ttcactataa aagccagagt
     3181 gccgacatac tggacaggat gcgcatgaag atacgccagc agcgatgcgc gatatggggc
     3241 agcaatgacc ttgccaataa tctgtttaat gacattcgca tgttcccaaa accaaagggg
     3301 atgcgctggg ctacgtttga caggaagcgg gcagagttac cagttatgga gatggcttac
     3361 tggcaggcat tcagcccggt agtggacaga atcacgggcg ttattgagcg taaagcagga
     3421 aaagcagcca gaggcattgg cctgactcta taaaagcaaa acgcccgcac ggggcgtcag
     3481 agaacgggat agacttctgc gacaatttgc attcgccgcc aatatagcaa gccgtccaca
     3541 tcaagcaata aactatcacc gggaggggga ggccaaatct ctgcaatcct gagccttaag
     3601 gactgcccgc cccatcgttt tttcataccc gcgaaaaatg aaatttaatc cgggcgtgtt
     3661 tcatccttcc agagggtatg acgtatgaca ccacgcgaaa tagccttatt gaccatcgcc
     3721 aaacttgagc atggaggcca ccagcttaca caggcagatc aacgggagat agaacgctca
     3781 gttaatgccg atatagcccg gcgcgacagg ttccgcgaaa tgatgcgagc acctgcctac
     3841 cagtggaaga agccagcgcc gcgcaggtag atacgggatt attccgcatg taacttatgc
     3901 gtattaagca aatttccttt attcaccttt acagaattgt gagtacgtca gtatcgttat
     3961 ccttccgatt cgcatttatt caggatttgc atgtaccagc taaagataac catccgagac
     4021 agcaaaccac ctatatggcg gcgtgtgctg gttccagagc aaatcccctt tagtgaactt
     4081 cacgcggtga tccagttggc ttttggctgg aatgacgaac acctgtatat gttcgagaaa
     4141 gggcgtaaag gtgatcccgg tagcgagtat cgcgtatggg gtgaggatga aagcatgggt
     4201 aatgcggcaa tcacaccact atgggcagcg ctccagaatg atggtgacaa gctggtttac
     4261 acgtatgact ttggcgactg gtgggattgc gtcattgtgc tggagaagca aacacacgat
     4321 acgagcaatc agcccattag ctgcctgcgt gggaaaggca ccaccccggt ggaaaactca
     4381 ggcggcttgc atggttacaa tgaattgcta ttacaggcca gagagtctga taatcctgag
     4441 caggccgaga tccacaattt cctgatgctg gatattgagc gccgggttta cgacctgagc
     4501 agcatcaacg acagattgca gactcttaac tgacccgcat atattcgcct gatattgcgt
     4561 tatcagcgcc gccagtatcg cgcataacag cgcactgacc gggcaaaccg tagcgcacga
     4621 ccgttgcgtt agtctcaccg ccgcgccgta tctggcgcga ttcatacact gtcacccgct
     4681 caagcagtcc accagcaacc atgctttcca gtgtgcgccg ggttgattcg agctggtgac
     4741 gcttatcgaa cgacaccatg ccatgaagca ggtaggccac acccgacaca tcgaacggcg
     4801 gcgcaccaat ctcaccagtc acccattcga ggttatccgg ttcaaagtag ctaagtatct
     4861 cttttttgcg gctggtcatt ctcatagctg gcaggttcct tattgtggga tagcactatc
     4921 atacaataag gcgcaaaaga taagaggcca gaacagcaag catgagagca taaaacgagc
     4981 agccaaagac agcgcctcaa aataccgtgt acacaagggt gtacctactg agaaaacgca
     5041 ttacaagaaa cacagcaacc acgggcgttt aaagccacta ctcaataaac agattcaact
     5101 cgtaacgcca attcttactt ttcagccttc acaaatgccg cccactcaaa cagagcggca
     5161 tttttcttcc ccgcaacttc aattctgttt tgcgtgcctg ctccagattt tgcgatgttt
     5221 ttttgcccag cacactgaga acgtgagata ctcacaactg tatataaata cagttacaga
     5281 tttactttct ttgcaatcga tatcacatgg agtgggcaat gaacgaacat catcaacctt
     5341 ttgaagaaat acggcattat ggcactgaag ggcaagaatt ttggtcagct agagaattgg
     5401 caccgttact ggattatcgg gactggcgca attttcaaaa ggtactggcc cgcgcgactc
     5461 aagcgtgcga agccagcaat caagctgcat ctgaccattt cgttgaaacc accaaaatgg
     5521 ttgtattagg ttcaggggct caacgagaac ttgaagatgt tcatctctcc cgctatgcct
     5581 gttacctggt agtacaaaac ggcgaccctg cgaaaccggt tattgcggca gggcaaactt
     5641 attttgctat ccagacccga cggcaggagc tggctgacga tgaagctttc aggcaacttc
     5701 gtgaagacga aaagcgtctt tttctgagaa atgaattgaa agaacataac aaacaattgg
     5761 ttgaggcagc gcagcaagct ggggtagcga cagctatcga tttcgccata tttcagaatc
     5821 atggttaccg ggggctgtat ggtggattag atcagaaagc tattcatcag cggaaggggc
     5881 tgaaaaagaa tcagaatatc ctggatcata tgggttcaac agaactggcg gctaatctct
     5941 ttcgagctac ccaaacagaa gaaaaactca agcgggatgg cgtgaattca aaacagcaag
     6001 caaacaccac acactttgac gtgggtcgca aggtgaggca aaccattcag gaacttggcg
     6061 gaaccatgcc tgaggagttg ccgaccccgc aggtcagcat caagcagttg gaaaacagtg
     6121 taaaaattac agagaagaaa tagcagaaac taaaaactct ttttcttaat tgagataccc
     6181 gatgcgtaag ccaaagtcca ggtgcagtga acacaatggc taaacattgc acctttcttt
     6241 tccacccctc aattttaacc tattttttct tatgcatttt ctcagacaag aactcagaat
     6301 aatgcatcta cgctacagaa aatagcgatt tcacataact acaagttatc aatttcctct
     6361 taccttaaaa aatctcaatc gtgacaatgc gcacaaatcg ctaccctgcc agacagattt
     6421 ttagggagag aaccatgctg ttacacattt tgtatttggt tggcatcact gccgaagcca
     6481 tgacgggggc gctggcggcc ggacgacggc gcatggatac atttggcgta attattattg
     6541 cgaccgccac cgcaattggc ggagggtcag tgcgcgatat tctgctgggc cactatccgc
     6601 tcggctgggt caaacacccg gaatatgtga ttatcgtcgc taccgccgca gtactcacca
     6661 ctatcgttgc tcctgtaatg ccttacctgc gcaaagtgtt tctggtgctc gatgcgctcg
     6721 gactggtggt cttttctatc atcggcgcac aggttgcact ggatatgggg cacggcccaa
     6781 ttattgccgt tgtcgcggcg gtaaccaccg gcgtgtttgg cggcgttttg cgcgatatgt
     6841 tctgtaaacg catcccactg gtattccaga aagagttgta tgctggtgtc tcatttgcct
     6901 ccgccgtgct gtacatcgcg ctgcaacact atgtttctaa ccatgatgtg gtgattatct
     6961 ccaccctggt attcggcttt tttgcccgct tactggcgct acgtcttaag ctgggattac
     7021 cggtctttta ctacagccac gaaggtcact aaggttcaaa acctgtgatc tgctgggcag
     7081 ccagccaact gcccagcttc ttgatttgcg cattttcctt ccattcaata acctgtctgg
     7141 cgcgcccaga tccagtcccc ggcagctgct gtcagaactg ctccgtgcta aataaaagtt
     7201 gcgactagga ccgttcatca ctggcattaa gcgccgcccg ggttagcggt attcccattg
     7261 ccatcaccca gcgagtaaaa ggctgcttac gagccagatt aaactgatgc cataactgcg
     7321 cacttttact tttcgcgatc cccggcgtgt tctgtaattg ctctggcgtt aataaaagcc
     7381 aagaaaagat atgctcaaag cgatgagtct gatgcagcgc gcgccaaccg gcctcaccaa
     7441 tgccatccag cccaagaacc tgttttgacc cca
//
LOCUS       DAJVUJ010000182         7409 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.182, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000182 DAJVUJ010000000
VERSION     DAJVUJ010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7409
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.182"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            1..1459
                     /locus_tag="OQQ48_003680"
                     /pseudo
     CDS             1..1459
                     /locus_tag="OQQ48_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
     gene            1470..2543
                     /locus_tag="OQQ48_003681"
     CDS             1470..2543
                     /locus_tag="OQQ48_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1954300.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHSTLDFSG
                     YLITANENKVGTTFNTTVTNGSSYPGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGAKA
                     IVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMANVYIRGDITAPQSCEIN
                     NLQPVYFDFKEIPAADFSSVVGSAVITHKITKTVTIECENLGILNTDDISTSFYATEP
                     NTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMGKSTVYGEKLGSVTFSAAPAS
                     LTGARPAPGQFTATATITVEIVR"
     gene            2791..3831
                     /gene="pstS"
                     /locus_tag="OQQ48_003682"
     CDS             2791..3831
                     /gene="pstS"
                     /locus_tag="OQQ48_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCS1954301.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            3918..4877
                     /gene="pstC"
                     /locus_tag="OQQ48_003683"
     CDS             3918..4877
                     /gene="pstC"
                     /locus_tag="OQQ48_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HCS1954302.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            4877..5767
                     /gene="pstA"
                     /locus_tag="OQQ48_003684"
     CDS             4877..5767
                     /gene="pstA"
                     /locus_tag="OQQ48_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCS1954303.1"
                     /translation="MAMVEIQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            5858..6631
                     /gene="pstB"
                     /locus_tag="OQQ48_003685"
     CDS             5858..6631
                     /gene="pstB"
                     /locus_tag="OQQ48_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HCS1954304.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            6646..7371
                     /gene="phoU"
                     /locus_tag="OQQ48_003686"
     CDS             6646..7371
                     /gene="phoU"
                     /locus_tag="OQQ48_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCS1954305.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
BASE COUNT         1863 a         1718 c         1933 g         1895 t
ORIGIN      
        1 cagatgttga tgtcagcgtg ggtgaattgc gcgatgaaga cggatttaca ccggacgtcc
       61 ttcaggcgca aatacttcat ggtttttccc acgggatcac tttatatgga ggtatgcagg
      121 ctgctgaaaa ttatggttct gcagctctgg gtgtcggtaa agatcttggc gctttgggcg
      181 caatttcttt cgatgtgaca catgctcgtg cgaattttag ccatgatgat acagaaacgg
      241 gtcagtcata tcgctttctc tattcaaaac gatttgacga cacagacact agcttgcgcc
      301 tggttggcta tcgttactcc accgagggct actataccct caatgagtgg gcatcgcggc
      361 gcaacagccc tgaagacttt tgggaaacag gtaaccgacg tagtcgcgtg gagggaacgc
      421 taacgcagtc gttggggaga gattatggca atttatacct gacattaagc cggcaacaat
      481 actggcatac cgatgatgtc gaacgattaa tgcaatttgg ctacagcagt agctggaagc
      541 gtctctcgtg gaacgtctcc tggagttatt ccaatactgc cagacagggg acggggaaca
      601 accatgccag tgataacacc agtgagcaga tctacatgct ctctttatct gttcctttat
      661 cgggctggtg gggtaatagt tacgccacct attctgtttc gcaaaacgat aattccggta
      721 gctcacatca agcggtacgg cgctggaaag aaataacctt tcatggaatt taatgcagtc
      781 ctataacagt catgatgatg aggttggcgg taatatgtcc ctgacctatg atggctctta
      841 tggcacggtg aacggcagct ataactacag ccaaaattcc cagaggctga attatggtat
      901 cagaggggga attctggcac acagcgaagg ggtaacgtta agtcaggagt taggtgaaac
      961 tattgctctt gttaaagcac ctggggccgc cgggttagaa atagataata tgcgcggtgc
     1021 tgcgacggac tggcgcggct atacggtcaa gacacagcta aacccttatg atgaaaatcg
     1081 ggtagcaatc agcgataact atttctcgaa gtcgaatata gaacttgata ataccgtcgt
     1141 tacgatggtt cccacgcgtg gtgcagtggt taaagcggag tttgtgactc atgtgggtta
     1201 tcgcgttctc ttcagagtgt taaatgcaaa tggtaaaccg gtaccttttg gagccattgc
     1261 tgcgatacaa gatgcaagtt tggcagattc aggaattgtc ggtgaccgtg gcgaacttta
     1321 tctttctggt ctaccagaaa aaggacaggt tacgttatcc tggggagaaa acgcctcaac
     1381 aaaatgcatc ttcaattatt cactttcgac accagaaagt gagagcggat taattgaaca
     1441 gggtgtgaca tgtcattaaa aggattttta tgaacaagta tatcaaacag tggtgctttg
     1501 ctgtgtttat gctctcgtta agtagtgtag cacttgcagc tcctaaaggt atctgtaccc
     1561 ctgataatgg cgtatttcac agtacgcttg atttctcggg atatcttatt accgcgaacg
     1621 aaaataaggt tggaacgaca tttaacacca ctgtaactaa tggatcctca tatcctggac
     1681 gctgtcattg tgatactggt aacgtaggag aattccccta tatctattat acgtcaaaaa
     1741 taaaccaggc attaacttat gctggggttc actctaatat taattattat gatcttaatc
     1801 cgaaccttga tgttgggatt gctatagata ttcttggggt tgggtatgtg aatgcacctt
     1861 ttgaatatca tgctaacaac ccgtctggta atacaaagta caattgcaac cgcattgaac
     1921 ctttaagtat atctagtggg gctaaagcga tagtatattt ttatatcaag aagacatttg
     1981 caggaaaatt gattattcct gaaacaaaga tagtgacatt gtatggaaca attagccgtg
     2041 acaccccggt ggattactca cagccgatgg ctaatgttta tattcggggc gatattacag
     2101 ctccgcaaag ttgcgaaata aataatttac agccagttta ttttgatttt aaagaaatac
     2161 cagctgcaga tttttcatct gttgttggaa gtgcggtaat aacacataaa attactaaaa
     2221 cggtcactat tgagtgtgaa aacctgggga tactgaatac cgatgatatc agtacgtctt
     2281 tttatgctac cgaacccaat acggacaatt caatggtcgt aacttcaaac tctaacgtgg
     2341 ggataaaaat ttacgacaaa aataataagg aaatcaaagt gaacggtggt gagttgccaa
     2401 cagacatggg taaatcaaca gtgtatggtg aaaaattagg tagtgtaact ttttcagctg
     2461 ctcctgcgag ccttactgga gctcgcccag ccccggggca atttactgca acagcgacga
     2521 taactgttga gattgtgcgc taataggtct gacaacgatt gttcatcaca tcgttgtcat
     2581 ctcacccatt tctttatcac ttttaaatgc atcatgacaa aaagatgaca tgtgggattt
     2641 tcatcttcac atttaaaatc agtaagttat ttggtaattc ctttctgtca taaaaatgtc
     2701 atattcctta catataactg tcacctgttt gtcctatttt gcttctcgta gccaacaaac
     2761 aatgctttat gaatcctccc aggagacatt atgaaagtta tgcgtaccac cgtcgcaact
     2821 gttgtcgccg cgaccttatc gatgagcgct ttctctgtgt ttgcagaagc aagcctgaca
     2881 ggtgcaggtg caaccttccc tgcgccggtg tatgccaaat gggctgacac ttaccagaaa
     2941 gaaaccggta ataaagttaa ctaccagggt atcggttctt ccggtggcgt aaaacagatt
     3001 atcgctaata ccgttgattt tggtgcctct gacgcgccgc tgtctgacga aaaactggct
     3061 caggaaggtc tgttccagtt cccgaccgtg attggcggcg tggtgctggc ggttaacatt
     3121 ccagggctga agtctggcga actggtgctg gatggtaaaa ccctcggcga catctacctg
     3181 ggcaaaatca agaagtggga tgatgaagcc atcgccaaac tgaatccggg tctgaaactg
     3241 ccttcacaaa acattgctgt agtacgccgc gcagatggct ccgggacttc cttcgtcttc
     3301 accagctacc tggcgaaagt gaacgaagag tggaaaaaca acgttggtac tggctctacc
     3361 gtaaaatggc cgatcggtct gggcggtaaa ggtaacgacg gtatcgccgc gttcgttcag
     3421 cgtctgccgg gtgcaattgg ttatgttgaa tatgcttacg cgaagcagaa caacctggcg
     3481 tacaccaaac tgatctccgc tgatggtaaa ccggttagtc cgaccgaaga aaacttcgct
     3541 aatgcagcaa aaggtgcaga ctggagcaaa accttcgctc aggatctgac caaccagaaa
     3601 ggcgaagatg catggcctat tacctctacc acgttcattc tgatccacaa agatcagaag
     3661 aaaccagaac aaggcacaga agtgctaaaa ttcttcgact gggcgtacaa aaccggggct
     3721 aaacaggcga acgacctgga ttacgccagc ctgccggata gtgtagttga acaggttcgc
     3781 gctgcgtgga agaccaatat taaagacagt agcggtaagc cgctgtacta ataaaactcc
     3841 aggccgggta cggtgtttta cgccgcatcc ggcattacaa aatgactttg taaacgcgtt
     3901 taactgaaga gtaacttatg gctgcaacca agcctgcttt taacccaccg ggtaaaaagg
     3961 gcgacataat tttcagcgtg ctggtaaaac tggcggcgct gattgtgcta ttgatgttgg
     4021 gtggcattat tgtctctctg atcatctcct cctggccgag cattcagaaa tttggtctgg
     4081 ctttcctatg gaccaaagag tgggatgcac cgaacgatat ctacggggcg ctggtgccga
     4141 tctacggtac gttggtgact tcgtttatcg cgctgctgat cgccgtcccg gtgagtttcg
     4201 gtatcgccct gttcctgact gagcttgcgc ctggctggct gaaacgcccg ctgggtatcg
     4261 ccattgagct gctggcagcc attccaagta tcgtttacgg catgtggggc ctgtttatct
     4321 ttgcgccgct gttcgccgtt tactttcagg agccggtcgg caatatcatg tcgaatatcc
     4381 cgattgttgg cgcgctgttc tctggccccg catttggtat cggtatcctc gcggcaggcg
     4441 tgatcctcgc catcatgatt attccgtaca ttgcggcggt aatgcgtgat gtgttcgaac
     4501 aaaccccggt gatgatgaaa gagtcggcct acggtattgg ctgcaccacc tgggaagtta
     4561 tctggcgtat cgttcttccg ttcaccaaaa atggtgttat cggcggcatc atgctggggc
     4621 tgggccgcgc gctcggtgaa accatggcgg tgacctttat catcggtaac acctaccagc
     4681 tcgacagcgc ctcgctgtac atgccgggca atagtatcac ctctgcgttg gcgaacgaat
     4741 ttgcggaagc ggaatccggt ctgcacgttg ccgcactgat ggaactgggc ctgatcctgt
     4801 ttgtgattac cttcatcgtc ctcgccgcat cgaagtttat gattatgcgc ctggctaaga
     4861 atgagggggc acgctaatgg ctatggttga aatccaaacc actgcggcgc tggctgaatc
     4921 tcgccgcaaa atgcaggcgc gtcgccgcct caaaaaccgt attgcgctga cgctctcgat
     4981 ggcgacgatg gccttcggcc tgttctggct gatctggatt ttaatgtcca ccatcactcg
     5041 cggtatcgac ggtatgtcgc tggcgctgtt cactgaaatg acgccgccgc ccaatacgga
     5101 aggtggtggt ctggcgaacg ccctggcggg tagcgggctg ttaattttgt gggccacggt
     5161 attcggtacg ccgctgggca ttatggcggg gatttatctg gcggaatatg gtcgtaaatc
     5221 ctggctggca gaagtgattc gcttcattaa cgacattctg ctctccgcgc cgtcgattgt
     5281 ggttggtctg tttgtttaca ccatcgtggt ggcgcagatg gagcacttct ctggctgggc
     5341 gggcgtgatt gccctggcgc tgttgcaggt gccgattgtt atccgcacca ccgagaacat
     5401 gctgaaactg gtgccggaca gcctgcgtga agcggcttat gcgctgggta caccgaagtg
     5461 gaagatgatc tctgcgatta cgctgaaagc gtcggtgtcc gggatcatga ccggtatcct
     5521 gctggcgatt gcccgtattg ctggtgaaac cgcgccgctg ctgtttaccg cgctctccaa
     5581 ccagttctgg agcacggaca tgatgcagcc gatcgccaac ctgccggtga cgatctttaa
     5641 gtttgcgatg agcccgtttg cggaatggca acaactggcc tgggccgggg tattgattat
     5701 taccctgtgc gtactgctgc tgaacattct agcgcgcgtt gtttttgcga agaataaaca
     5761 cggttaacac tttgccggat gcggcgtgaa cgccttatcc ggcctacggt aagcctgatt
     5821 agcgaagtgc atcaggcacg atgaggaaaa gattgcaatg agtatggttg aaactgcccc
     5881 gagtaaaatt caggttcgta atttgaactt ctactacggc aaattccatg ccctgaaaaa
     5941 catcaacctg gatatcgcta aaaaccaggt tacggcgttt atcgggccgt ccggctgcgg
     6001 taaatcgacg ctgctgcgta ccttcaacaa aatgtttgaa ctgtacccgg agcagcgtgc
     6061 ggaaggtgaa attctgcttg atggcgacaa catcctgacc aactctcagg atatcgcact
     6121 gctgcgtgcg aaagtgggca tggtgttcca gaaaccgacg ccgtttccga tgtccatcta
     6181 cgacaacatc gcttttggcg ttcgtctgtt tgagaagctc tcccgtgccg acatggacga
     6241 gcgcgtgcag tgggcattga ccaaagccgc attgtggaac gaaaccaaag ataaattgca
     6301 ccagagcggt tactctctct ctggtggtca gcaacagcgt ctgtgtattg cgcgtggtat
     6361 cgccattcgc ccggaagtgc tgctgctcga cgaaccgtgt tcggcgctcg accctatctc
     6421 taccgggcgt attgaagagc tgatcaccga actgaagcag gattacaccg tggtgatcgt
     6481 cacccacaac atgcagcagg ctgcgcgttg ttccgaccac acggcgttta tgtacctggg
     6541 cgaattgatt gagttcagca acacggacga tctgttcacc aagccagcga agaaacaaac
     6601 agaagactac atcaccggtc gttacggttg attcaggagt gcgttatgga cagtctcaat
     6661 cttaataaac atatttccgg ccagttcaac gccgaactgg aaagtatccg cacgcaggtg
     6721 atgaccatgg gcggcatggt ggagcagcag ctttctgatg caatcaccgc gatgcataac
     6781 caggacagcg atctggcgaa gcgcgtgatc gaaggcgaca agaacgtcaa catgatggaa
     6841 gtggcgatcg atgaagcctg cgtgcgcatt atcgccaaac gtcagccgac ggcgagcgac
     6901 ctgcgactgg ttatggtgat cagtaaaacc attgccgagc tggagcgtat tggcgacgtg
     6961 gcggacaaaa tctgccgtac tgcgctggag aaattctccc agcagcatca gccgttgctg
     7021 gtaagtctgg agtcgctggg ccgtcatacc atccagatgc tgcacgacgt gctggacgcg
     7081 ttcgcgcgga tggacattga cgaagcggta cgtatttatc gtgaagataa aaaagtcgat
     7141 caggaatacg aaggtattgt tcgtcaactg atgacctaca tgatggaaga ttcgcgtacc
     7201 attccgagcg tacttactgc gctgttctgc gcgcgttcta tcgaacgtat tggcgaccgc
     7261 tgccagaata tttgtgagtt tatcttctac tacgtgaagg ggcaggattt ccgtcatgta
     7321 ggcggtgatg agctggataa actgctggcg ggaaaagata gcgacaaata attcaccaga
     7381 caaatcccaa taacttaatt attgggatt
//
LOCUS       DAJVUJ010000183         7387 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.183, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000183 DAJVUJ010000000
VERSION     DAJVUJ010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7387
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.183"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            566..3979
                     /locus_tag="OQQ48_003687"
     CDS             566..3979
                     /locus_tag="OQQ48_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCS1954306.1"
                     /translation="MEQSLNFEMLRSQWPELAELACMAERYVHSDPESCLVKLRNYTE
                     LMVRWLYRQERLPEGIKANLYDLMNADVFTSMMPEAIIMKMDALRIHGNRAAHGGRIK
                     AKDTYWLLKEAYLLGIWLYVRYAHGNVDDCPKFTLPPLTQSSGRADEKRLEDAIKAQD
                     ESRERELALQRALQQEQEKAEHLTQRLNEARARNQHVADILSIDEAETRRRLIDSRLL
                     AADWNVGEELKNTDQVTQEHPVKEQPTTSGDGYADYVLWDEAHKPLAVVEAKKTSVNA
                     EQGRIQARLYADWLEKEYGQRPVIFYTNGYDIWLWDDHKTHGYPPRRVFGFYSKESLQ
                     YLIQQRETRLPLNRVPHVKDNEGKAVAGRLYQLETIARVSERFTNKYRQSLIVQATGT
                     GKTRVAIALSKLMIDARWVKRVLFLCDRKELRKQAANAFNQFTNEPLYVVGKSKKADR
                     QNARIYIATYPGMMKIMDHFDVGYFDLIIADESHRSIYNVYGDLFKYFDALQIGLTAT
                     PIDMVSKTTFGLFGCEGRIPTANYSLEDAIADNNLVPYEVVTHTTEFLREGIKREKLS
                     DAQIRELEEQGIDPNTLEFDGKALDEAIYNKDTNRYILRNLMENGLKDRDGQLPGKTI
                     IFARNHKHALLLNELFDDMYPQFAGRFCQVIDNYDPRAEQLIDDFKGLDESTNKELTI
                     AISVDMLDTGIDVPEIVNLVFAKPVKSKVKFWQMIGRGTRLCPGLYGYDDNGKPLDKQ
                     KFRIFDHWGNFEYHELHTEEAEVTATKSLAQKRFEAWVMLGAAAQRKFDKQAVDLVAH
                     QLREQINALDEKSIAVQEKWQQKAQYSDEKVLRQLSPKTQQDLLSVLAPLMQWLDVRG
                     QSDAIRFDMDILAAQTARYTNPEELDVLWPIIVEKVERLPPHLAQVQQQGQRINQLRN
                     LSWWKQASLEELEDIRIHLRGIMHLMEKDATPKFGSIQVDITEDANLIQTETRKTNIR
                     SIDFKLYRQQVQGALEPLFQQNPVLKKIRNGEPVTQNELDELAKLVLIQNPNVDIRAL
                     KEFYPQATASLDKLLRTIIGMDSDAVEVRFAQFAADNSLTSQQLRFLSLLKNHIRDYG
                     TIEMRQLFEQPFTHIHNEGVTGVFPDIAQIARLQKIVEELGVVTDAATV"
     gene            4043..5677
                     /locus_tag="OQQ48_003688"
     CDS             4043..5677
                     /locus_tag="OQQ48_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HCS1954307.1"
                     /translation="MITGDLKSKIDGLWEDFWVGGITNPLTVIEQITYLMYSRMLDTQ
                     EQRDEKRKQIAGIDFKPRFAPEQQEFRFSHYSNLGSDEMMEVVRDGVFQHFRQLGQAD
                     ASKVTLLGNFMKDARLEIVKPSLLTKAVEVIKNLPLDRGDTKGDLYEYLLSKLTTAGI
                     NGQFRTPRHIIRTMVEMMEPNPARGETICDPACGTGGFLATSYEYLLEKYSSLESIHT
                     EIGTNERGELEEQKIFTGDLLTPWRNHVDNNMFHGYDFDTTMLRIAAMNLIMHGVDAP
                     DIHYQDTMSQSFSTNFPQASKNAFNLILANPPFTGSLDEEDIDSTLSAMVKTKKTELL
                     FLARILQMLKVGGRSATIVPQGVLFGSSKAHQSLRKTLVEDNQLETVINLPSGVFKPY
                     AGVATAILIFTKGGQTDEVWFYDLQNDGYSLDDKRNPIKDNDLPHLLASWKHYRTLRG
                     LPVDNFMGKKLASLLKQQYPEGINAGVDFKDRTQAAFVVPKADIAAQKYDLSINRYKE
                     VVYQAEEYEDPKVILKRLKDLEKEILADLDELEGML"
     gene            5686..6756
                     /locus_tag="OQQ48_003689"
     CDS             5686..6756
                     /locus_tag="OQQ48_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001438891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCS1954308.1"
                     /translation="MVKLGDVINVHYGKALKADQRVSNGSVHVFGSSGIVGNHDKTLC
                     SYPTIIIGRKGSVGAITWAPSGGWIIDTAYYVEIKDNNKLDLRYLFYILSGIDLTKKT
                     ITTSIPGLNRDDLYDTFIKLPPFEEQKRIVDLLDKAEGIRQKREQSIKLADDFLRATF
                     ATMYGNPITNPKKWPVHLMGEIIEFKGGNQPPKSDFIFEPKQGYIRLVQIRDFKSDKY
                     ATYIPQEKAKRIFEVDDVMIARYGPPVFQILRGLSGSYNVALMKASPKENIRKGFIFY
                     LLQLPEYHDVVVKNSERTAGQTGVNLELLNKFNVPLPPIYYQDEILDRLARIEKFKEK
                     IEISLNHLEIQFLSLQKRLIGF"
     gene            complement(6881..7096)
                     /locus_tag="OQQ48_003690"
     CDS             complement(6881..7096)
                     /locus_tag="OQQ48_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005024529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1954309.1"
                     /translation="MGVNELYLAYDFSFFLRMMPYMEPGLIKRDMGELLKILKKHIDE
                     AMNTQVLSSNFCKLVCIYDYIKNSHRY"
BASE COUNT         2154 a         1679 c         1761 g         1793 t
ORIGIN      
        1 gtcctgttct cccaaatatc atggtattga tacgggagat tatgccgtac aggtacgaca
       61 cgttatgtcg tgccaaaggg gagtggatag aaaaaatggc gaagatgcga aaagttgatt
      121 aatgcattga aaaaaaatga attaataatg aacttgggta aaagcgccat caaaatacga
      181 cacgacctgt cgtattttgt gttagccggg aaaagatatc tgctacttac taaaatgatt
      241 tagtgctgaa aatatttata agccaataaa tagacaggta ttacggagta acgcccaggc
      301 tattttaacg agcctgctgt tttattggct caaaaaacat atcccataga tgatcagcca
      361 tacccgcgtt agtgagcaag gtatcccttg agtagccagg gctgaagacg cccattgatg
      421 ggcgaaaacc aaggttctgg ccgttaaagc ggctatgtcg ttaaacacat caccaccata
      481 tgccatccgg ctcacttcgt cggccaaatt agtgtgatat tctttccaga atttctgttg
      541 tatccacaac ataaggcgat gattgatgga gcagtctctc aattttgaaa tgttgcgcag
      601 ccagtggccg gaactggcag aactcgcatg tatggcagag cgctatgttc actccgatcc
      661 ggaaagctgc ctggttaagc tgcgcaacta caccgaattg atggtgcgct ggttgtatcg
      721 tcaggagcgg ttgccggaag gtattaaggc taatctttac gatttaatga acgctgatgt
      781 ctttaccagc atgatgccgg aagccatcat catgaaaatg gatgcgctgc gtatccatgg
      841 caaccgtgcc gcgcacggcg gacgtatcaa agctaaagat acttactggc tgctcaaaga
      901 agcgtatttg ttgggaattt ggctgtatgt tcgctacgcc cacggtaatg ttgatgactg
      961 ccccaaattt acactccctc cattaacaca atcttcaggt cgtgcagatg aaaaacgtct
     1021 ggaagatgca atcaaggctc aggatgaaag ccgtgagcgc gaactggcgc tacaacgcgc
     1081 actacagcaa gaacaggaaa aggccgaaca cctcactcaa cgcctgaatg aagccagagc
     1141 gcgtaaccag catgttgccg atattctctc tattgatgaa gcggaaaccc gccgtcgcct
     1201 gattgactct cgcttacttg cagccgactg gaatgtaggg gaagaactta aaaacacgga
     1261 tcaagtcaca caagaacatc cagtcaaaga gcaacccacc acctctggtg acggttatgc
     1321 agactatgtc ttgtgggatg aggcacacaa accgttggcg gtggtggaag caaaaaaaac
     1381 cagcgtcaat gccgagcaag gacgaattca ggcccggctg tatgcagact ggctggaaaa
     1441 agaatacggt cagcgtccgg tcatcttcta caccaacggc tatgatatct ggctgtggga
     1501 tgaccataaa actcatggtt atcccccgcg tcgggtgttt ggcttctaca gcaaggaaag
     1561 cctgcaatat cttattcagc agcgtgaaac ccgtctgccg ctgaacagag tgccacacgt
     1621 aaaagataac gaaggtaagg ccgttgccgg acgtttgtac cagctcgaaa ccattgcccg
     1681 cgtcagtgaa cggtttacca acaagtaccg acaatcgtta attgttcagg ctacgggtac
     1741 aggcaaaacc cgcgtggcaa ttgcactaag caaactgatg attgatgccc gctgggtaaa
     1801 acgcgtcctg tttctttgcg accgtaaaga actgcgtaaa caggcggcaa atgcctttaa
     1861 tcaattcacc aatgagccgc tgtatgtggt cgggaaatcg aaaaaagcag acaggcagaa
     1921 tgccagaatc tacatcgcta cctatcccgg tatgatgaaa atcatggacc atttcgatgt
     1981 cggttatttc gatttgatca tcgccgatga atcccaccgc tctatctaca acgtctacgg
     2041 cgatctgttt aagtattttg atgccctcca gattggcctg acggccacgc caattgacat
     2101 ggtgagtaaa accactttcg gcctgtttgg ttgtgaagga cgtattccta ccgccaacta
     2161 cagcctggaa gatgccatcg ccgataacaa tctggttccc tatgaggttg tcacacacac
     2221 aacagaattc ctgcgcgaag gtatcaaacg ggagaaatta agcgacgcac aaatccgcga
     2281 gctggaagaa caaggcatag atcccaatac gctggagttt gatggcaagg cgctggatga
     2341 ggcgatctac aacaaagaca ccaaccgcta tatcctgcgt aacctgatgg aaaatggcct
     2401 gaaagatcgg gacggccagt tgcctggtaa aaccatcatt ttcgcccgta accacaaaca
     2461 cgcgctgctg ttgaatgaat tgttcgacga tatgtaccca cagtttgccg ggcgcttctg
     2521 ccaggtcatc gacaactacg atccccgcgc cgagcaactg attgatgatt ttaaagggct
     2581 ggatgaaagc accaacaaag agctgaccat cgctatctcc gttgacatgc tcgataccgg
     2641 tattgatgtc ccggaaattg tgaatctggt ctttgccaaa ccggtcaaat caaaagtgaa
     2701 gttctggcag atgattggcc gtggtacgcg cctctgtccg gggttgtacg gttacgacga
     2761 taacggcaaa ccgctggata aacagaaatt ccgcattttc gatcactggg gcaattttga
     2821 gtatcacgag ctgcataccg aagaggcgga agtcacagcg acaaaatcgc ttgcgcaaaa
     2881 acgcttcgaa gcgtgggtta tgctgggggc cgctgcgcag cgtaaattcg acaaacaggc
     2941 ggtggattta gttgctcacc agcttcgcga gcaaatcaac gcactggatg aaaagtcgat
     3001 tgccgtacag gaaaaatggc agcagaaagc gcagtacagt gatgaaaagg tgctgcgcca
     3061 gctttctccg aaaacacagc aggatctgct atctgttctg gccccgttga tgcagtggct
     3121 ggacgtgcgc gggcaaagcg atgccatacg ctttgatatg gatattctgg cggcgcaaac
     3181 tgcccgttat accaacccgg aagagctgga tgttctctgg ccgatcatcg tcgaaaaagt
     3241 ggagcgcctg ccgccgcatt tggcgcaggt gcagcaacag ggacaacgaa tcaatcagct
     3301 tcgtaattta agctggtgga agcaggccag cctggaagag ctggaggata ttcgcattca
     3361 tttgcgcggt atcatgcacc tgatggaaaa agacgcgacg cctaaatttg gttcgataca
     3421 ggtggatatt accgaagatg cgaacctgat ccagacggag acccgcaaaa ctaatatccg
     3481 ctcgattgat ttcaaactct atcgccagca ggttcagggg gcgctggagc cgctgttcca
     3541 gcaaaacccg gttctgaaga aaatccgcaa cggcgagcca gtcacgcaaa acgagctgga
     3601 tgagctggcg aagctggtgc tgatccagaa ccccaacgtt gatattcgtg cgttgaaaga
     3661 gttttatccg caggcaaccg ccagcctgga taaacttttg cgtaccatca tcgggatgga
     3721 cagcgacgcg gtggaagtgc gttttgccca gttcgccgct gataacagcc tgaccagcca
     3781 acaactgcgc ttcctgtcat tgctgaaaaa ccacattcgc gattacggca ccattgaaat
     3841 gcggcagctc tttgaacagc cttttacgca tatccacaac gaaggcgtta ccggtgtgtt
     3901 ccctgatata gcgcaaatcg cccgcctgca aaagatagtc gaagagctgg gtgttgtgac
     3961 cgacgcagcg acggtataat acacgcatga caagccccgg cagcggggct tttgtattta
     4021 aaacaggata taaacgctaa caatgattac tggcgactta aaaagtaaaa tcgacggact
     4081 atgggaagat ttctgggtgg gtggtatcac caacccgctg accgtaatcg aacagatcac
     4141 ctatctgatg tattcccgga tgctggatac ccaggaacaa cgcgacgaaa agcgtaaaca
     4201 aatcgcgggt attgatttta aaccgcgttt tgcgccagaa cagcaggagt tccgtttcag
     4261 tcactatagc aaccttggct cggatgagat gatggaagtg gtgcgcgatg gcgtattcca
     4321 gcatttccgt cagctcggcc aggccgatgc ttcgaaggtg acgctgctgg gcaactttat
     4381 gaaagatgct cgtctggaga ttgttaagcc gtcattgctg accaaagcgg ttgaggtaat
     4441 caaaaacctg ccattggatc gcggcgacac caaaggcgac ctttacgaat acctgttaag
     4501 caagctgaca actgccggaa tcaacggaca gttccgcaca ccgcgccaca ttatccgcac
     4561 gatggttgaa atgatggagc cgaacccggc ccgcggcgag acgatttgcg atcccgcctg
     4621 tggcaccggt ggttttctgg caaccagcta tgaatatctg ctggagaagt acagctcgct
     4681 ggaatccatt catactgaga ttggcaccaa cgaacgtggc gagctggaag agcaaaaaat
     4741 ctttaccggc gatctgctga caccgtggcg taaccatgtg gataacaaca tgttccacgg
     4801 ttacgacttt gacaccacaa tgctacgtat cgccgccatg aacctgatta tgcacggcgt
     4861 ggatgcgcct gatatccact atcaggacac aatgagccag agtttcagca caaacttccc
     4921 gcaggccagt aaaaacgcct tcaacctgat tctggcgaac ccgccgttta ccggttcact
     4981 ggacgaggaa gatatcgact ccacgctgtc ggcaatggtg aaaaccaaaa aaaccgaact
     5041 actgttcctg gcgcgtattc tgcaaatgct gaaagtgggc gggcgcagtg ccactatcgt
     5101 gccgcagggc gtgctgtttg gctctagcaa ggcgcaccag tcacttcgca aaacgctggt
     5161 ggaagataac caactggaaa cggtgatcaa tctgccttct ggtgtattta aaccttacgc
     5221 tggcgtggcg acggcgatcc tgatctttac caaaggcggt caaacggatg aggtctggtt
     5281 ctacgatcta caaaatgacg gctacagcct ggatgataag cgcaacccga taaaagacaa
     5341 cgatctgccg catctgctgg caagctggaa gcattaccgt actttacgcg ggctaccggt
     5401 tgataacttt atgggtaaga agttagcctc gttgcttaaa cagcagtacc cggaagggat
     5461 taatgctggc gttgatttta aagatcgcac gcaggcggcg tttgttgtac cgaaagcgga
     5521 tattgctgcg cagaaatacg atctatccat caaccgttat aaagaagtcg tgtatcaggc
     5581 ggaggaatat gaagatccga aggtgatatt gaagcggtta aaggatctgg aaaaagagat
     5641 tctggcggat ttggatgagc tggaggggat gctgtgagtt cggcgatggt gaagttaggg
     5701 gatgttatta atgttcacta cggaaaagca ttaaaagctg atcagcgtgt atcgaatgga
     5761 agtgtccatg tgtttggtag ttcaggaatt gtcggcaacc acgataaaac gctttgcagc
     5821 tatcccacga taattattgg ccgcaaaggc agcgttggtg ctattacctg ggctccaagc
     5881 ggaggctgga tcattgatac tgcttattac gttgagatca aagataataa taagttagat
     5941 ctccgatact tgttttatat tctgagcgga attgatctca ctaagaaaac gattaccaca
     6001 agtattcctg gattaaatag agacgatctc tatgatacat ttattaaatt accacctttt
     6061 gaagagcaga aacgaattgt ggatcttctc gataaagctg agggtatacg ccaaaaacgt
     6121 gagcaatcca tcaaactggc tgatgatttt ttgcgtgcta cgtttgctac aatgtatggg
     6181 aatcctatta caaaccccaa aaaatggcct gtccatctta tgggagaaat tattgaattt
     6241 aaaggtggaa atcaaccacc taaaagtgat tttatttttg aacctaaaca gggttatatt
     6301 agattagtac aaattagaga ttttaaatcc gataaatatg cgacgtacat tccgcaagaa
     6361 aaagcaaaac gtattttcga agttgatgat gttatgattg ctcgttatgg accacctgtg
     6421 tttcaaatac ttagaggttt atccggcagc tacaatgtag ctttaatgaa agcttcaccc
     6481 aaagaaaata ttaggaaggg gtttatattc tatcttctac aacttccgga atatcatgat
     6541 gttgttgtaa aaaactctga aagaacagca ggacaaaccg gtgtaaatct tgaactatta
     6601 aacaaattca atgttcccct cccacctata tactatcaag atgagatatt agatagatta
     6661 gctagaattg aaaaatttaa agaaaaaata gaaatatcat taaatcattt agaaatacaa
     6721 tttttatcac tacagaagag attaataggt ttttaaatcc ttagtatcga aataaacatt
     6781 aatttattat attaaattaa gatattttag gaaattaagg cgtcactctt aggtaacgcc
     6841 tttttttact atctcgcccc ctaagaaggt tatatcttac ttaatacctg tgactatttt
     6901 tgatataatc ataaatacac actaatttgc aaaaattact tgacaacacc tgagtattca
     6961 tagcttcatc tatatgtttt ttcaatattt tgagaagctc tcccatatcg cgcttgatta
     7021 atccaggctc catataaggc atcattctta aaaagaaact gaaatcataa gcaagatata
     7081 actcattgac ccccaccttg acctcaggaa cggtttcaga gcgtcgttta tcccatcctt
     7141 gagactgaaa actaatagga gtgatattta atcgtgataa aacgccacct gagagttctg
     7201 tttcattaat tatactccca tcaggattaa ttaatttagc gtgatttaag aagtgaaaaa
     7261 taggaatgcg cgcattgcct ttgcatatat ttggcaaaac atcattaaca acacatcccc
     7321 aatcataata tccattttca aatacaacct ctcggatgag atctacacgc tccagtccaa
     7381 ccaatcc
//
LOCUS       DAJVUJ010000184         7321 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.184, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000184 DAJVUJ010000000
VERSION     DAJVUJ010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7321
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.184"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            157..264
                     /locus_tag="OQQ48_003691"
     CDS             157..264
                     /locus_tag="OQQ48_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1954310.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(317..1075)
                     /locus_tag="OQQ48_003692"
     CDS             complement(317..1075)
                     /locus_tag="OQQ48_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HCS1954311.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVTYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            1362..2291
                     /gene="pfkB"
                     /locus_tag="OQQ48_003693"
     CDS             1362..2291
                     /gene="pfkB"
                     /locus_tag="OQQ48_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HCS1954312.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISASQKQG
                     IRCIVDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            2392..2682
                     /gene="ghoS"
                     /locus_tag="OQQ48_003694"
     CDS             2392..2682
                     /gene="ghoS"
                     /locus_tag="OQQ48_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HCS1954313.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            2788..3648
                     /locus_tag="OQQ48_003695"
     CDS             2788..3648
                     /locus_tag="OQQ48_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCS1954314.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(3689..4225)
                     /locus_tag="OQQ48_003696"
     CDS             complement(3689..4225)
                     /locus_tag="OQQ48_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HCS1954315.1"
                     /translation="MTYQQAGHIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            4372..5040
                     /gene="hxpB"
                     /locus_tag="OQQ48_003697"
     CDS             4372..5040
                     /gene="hxpB"
                     /locus_tag="OQQ48_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HCS1954316.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            5203..5793
                     /locus_tag="OQQ48_003698"
     CDS             5203..5793
                     /locus_tag="OQQ48_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCS1954317.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQIINTLQIQFHRLIKHQVEY"
     gene            5926..7317
                     /gene="tcyP"
                     /locus_tag="OQQ48_003699"
     CDS             5926..7317
                     /gene="tcyP"
                     /locus_tag="OQQ48_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HCS1954318.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLAA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
BASE COUNT         1839 a         1788 c         1812 g         1882 t
ORIGIN      
        1 tatatgttga tgcataatat tattttttca taaaacgcag aaacagtgac caggtgtaca
       61 acgccaccca acggctgtaa taacaaaaaa tcccttgatg cccgtccctt ttgttacact
      121 ccgttatcac gcacaagaga tatgcaggac actggtatgc cgactaaacg ctttgataaa
      181 aaacactgga agatggtggt ggtgctactg gcaatctgtg gcgctatgtt gttgctacgt
      241 tgggcagcaa tgatttgggg ctgagcagtg acaaacgaag acagccagac cgcataacac
      301 ggtccggcag ataattttac atgctgtagc ccagcgataa ggttgtccga cggtcggtat
      361 gttccggtgc agactctgga ggttcagagt tccatgtaac gttgtaagtc accttcagtc
      421 caaaatgttc attaatagca acatttaatg cgctttcgga gttcagcgtt gtgtcttccg
      481 cgccaaagac cgaaacaccc tgcgtaaatt tagcgttgtc agtcaactgc catgcataag
      541 ccccggaggc atagcccagc ggctgagttt cactggcatt gtcggtatat ttgtcgtaac
      601 gcacacctgg accgaattca aagcggaaac tgtgtaccgg gccattcaaa aactgacgac
      661 cataacccgc ggtcaacaca tcgcgctctc gatagccgtt ataacggtct gtcagccagc
      721 ttgcctgacc aaataaatag tcataatcag ttaaattaaa gcggctacgc ccgcccgccg
      781 catatttttc tgaagaacgc tcatcattag aagaagtatt actggcgttc ccccacagcg
      841 accaggcagt ggtttgtcca taccaggtca tggtggtgtc agccgtaagt gaggagcttt
      901 tcgtattgcc tgattgtgca agatatcctg cgttcagatt accttcgaaa ggttttttag
      961 cgctggcagg atcgtccatg acagtaaaaa cggaatcatc ggcagctgca ttcagtgacg
     1021 caaacatgcc ccccgccaac ataacgatgg caggaactgt cttcaaaagc ttcatttatc
     1081 aagagtccgt acaacaaaaa aagagaccat cgcggtcccg gaaactttct taaggatcaa
     1141 agattagcgt ccctggaaag gtaacgaatt ataaaaaggc gcgaataact tagcaatgta
     1201 ttcttatttc attttttgaa taagcatgtg gcgaaaacag atttttattt atatatattt
     1261 atctgcaaaa ttttaaataa agctccaata aatcatattg ttaatttctt cactttccgc
     1321 tgattcggtg ccagactgaa atcagcctat aggaggaaat gatggtacgt atctatacgt
     1381 tgacacttgc gccctctctc gatagcgcaa caattacccc gcaaatttat cccgaaggaa
     1441 aactgcgctg taccgcaccg gtgttcgaac ccgggggcgg cggcatcaac gtcgcccgcg
     1501 ccattgccca tcttggaggc agtgccacag cgatcttccc ggcgggtggc gcgaccggcg
     1561 aacacctggt ttcactgttg gcggatgaaa atgtccccgt cgctactgta gaagccaaag
     1621 actggacccg gcagaattta catgtacatg tggaagcaag cggtgagcag tatcgttttg
     1681 ttatgccagg cgcggcatta aatgaagatg agtttcgcca gcttgaagag caagttctgg
     1741 aaattgaatc cggggccatc ctagtcataa gcggaagcct gccgccaggt gtgaagctgg
     1801 aaaaattaac ccaactgatt tccgcttcgc aaaaacaagg gatccgctgc atcgtcgaca
     1861 gttctggcga agcgttaagt gcagcactgg caattggtaa catcgagttg gttaagccta
     1921 accaaaaaga actcagtgcg ctggtgaatc gcgaactcac ccagccggac gatgtccgca
     1981 aagccgcgca ggaaatcgtt aatagcggca aggccaaacg ggttgtcgtt tcccttggtc
     2041 cacaaggagc gctgggtgtt gatagtgaaa actgcattca ggtggtgcca ccaccggtga
     2101 aaagccagag taccgttggc gctggtgaca gcatggtcgg cgcgatgaca ctgaaactgg
     2161 cagaaaatgc ctctcttgaa gagatggtcc gttttggcgt agctgcgggg agtgcagcca
     2221 cactcaatca gggaacacgt ctgtgctccc atgacgatac gcaaaaaatt tacgcttacc
     2281 tttcccgcta acaaaaacat tcccccagca ttgggggaat caccaccaac ctgtcggcaa
     2341 cgcgtttctc cgactatgct caaaagtcat gtgataacaa aggggtgaac tatggccagt
     2401 ggcgatcttg tccgttatgt cataaccgtc atgttgcatg aggatacatt gactgaaatt
     2461 aacgagttga ataattacct gactcgcgac ggttttttgc tcaccatgac ggatgatgag
     2521 ggaaatatcc atgagctggg gactaacact tttggactta tcagtaccca aagtgaagaa
     2581 gaaattagag aactggtttc ggggcttacc caaagtgcaa ccggcaaaga tcctgaaatc
     2641 accatcacga cctgggagga atggaatagc aacagaaaat aaatggtttt tgggcaataa
     2701 tcagtctgtg gtgtgcgtta gctcgtgttt ttacaccgca ttcttgcgct aaccttatga
     2761 tctggcagac aacatgggag agacatcatg tggcaggcaa tcagtcgtct tttgagcgag
     2821 cagttaggtg aaggcgaaat cgaactgcgt aatgaactgc ctggcggaga agtccatgcc
     2881 gcatggcatt tgcgctatgc aggacatgac tttttcgtca aatgtgatga aagggaactg
     2941 cttcccggtt ttaccgccga agccgaccaa ctggagttac tgtcgcgtag taaaaccgtc
     3001 accgtgccta aggtttgggc agtaggcgct gaccgtgact acagttttct ggtgatggat
     3061 tatctcccac ctcgtccgct ggatgcgcat agcgcattta ttcttggtca gcaaattgcg
     3121 cgtttacatc aatggagtga ccaaccacaa tttggcctcg attttgataa ctcgctctcc
     3181 acaactccac agcccaacac ctggcaacgt cgctggtcaa cgttttttgc tgaacaacgg
     3241 attggctggc agttggaact ggcagcagag aaagggatcg ctttcggcaa tatcgacgcc
     3301 atcgtcgagc atattcagca gcgtctggcc tcgcatcaac cgcagccttc tctgttgcac
     3361 ggcgatttat ggtccggcaa ctgtgcgctg ggtccggatg gcccgtacat tttcgaccct
     3421 gcctgctact ggggtgaccg agagtgcgac ctggcgatgt taccgctgca tactgaacag
     3481 ccgccacaaa tctatgacgg ctatcagtca gtatccccgc tacctgccga tttccttgaa
     3541 cgtcaaccgg tttaccaact ctacacgctg ttaaatcgtg caaggttatt tggcggtcag
     3601 catttggtta ttgctcagca gtcactggat agattattag cagcatgata tgggttgaag
     3661 ataatggccg ctctgtgcgg ccttttgatt aaataaaccc aagcagagaa aagaacacat
     3721 aacctgcagc aatacaaata accggcagga tatacagtga gaaaaactgc aggaaaatag
     3781 tatggtgcgg aacaacaata cgggactcaa tttgttcacg cgttagcccc tcttcccctt
     3841 tggctttttc cagaatgagt tgatcttcaa caccttcccg caagaagcgc gcctggcgac
     3901 tcattcgggc accggaatcc tgcaacgcca gtccgacaaa aatcaggatg aaaatcaccc
     3961 agaacataac gttcacgcca ccattaaaat tgggcgtcgg ggagttatac cagaacagat
     4021 tcaaaaaagg cgtattcgcc tgcatcatat cgatcatgac atgagtaaag tcgagcatga
     4081 ccgcattaat gccttcctgg ttttcctgcc gggtattcat aaatttcagc aaagaaatca
     4141 atgtagagat caatgcaggt ataaaaatca cccaacccaa aatccttttc aaaacagcaa
     4201 tgtgtccagc ttgttgatac gtcatgagtt ctccttgatt aagacgcgtc gtttcactta
     4261 gtttacctgt agatatctgt tttcgcccat tctttaaagg cgatatgata ggcgcttaat
     4321 cataagcacg gcttaatacc ttacacataa tgctctaaag gagaggttgt aatgtcaacc
     4381 ccgcgtcaga ttcttgctgc aatttttgat atggatggat tacttatcga ctcagaacct
     4441 ttatgggatc gagccgaact ggatgtgatg gcaagcctgg gggtggatat ctcccgtcgt
     4501 aacgagctgc cggacacctt aggtttacgc atcgatatgg tggtcgatct ttggtacgcc
     4561 cggcaaccgt ggaatgggcc aagccgtcag gaagtagtag aacgggttat tgcccgtgcc
     4621 atttcactgg ttgaagagac acgtccatta ttaccaggcg tgcgcgaagc tgttgcgtta
     4681 tgcaaagaac aaggtttatt ggtgggactg gcctccgcgt caccactaca tatgctggaa
     4741 aaagtgttga ccatgtttga cttacgcgac agtttcgatg ccctcgcctc ggctgaaaaa
     4801 ctgccttaca gcaagccgca tccgcaagta tatctcgact gcgcagcaaa actgggcgtt
     4861 gacccactga cctgcgtggc gctggaagac tcggtaaatg gcatgatcgc ttctaaagca
     4921 gcccgcatgc gttccatcgt cgttcctgcg ccagaagcgc aaaatgatcc acgttttgta
     4981 ttagcaaacg tcaaactctc atcgctgaca gaactcaccg caaaagacct tctcggttaa
     5041 tgaccagggg cagtgatcac gctgcccctg gttctttatc tgaatttgca ttcaactgac
     5101 ggattaatcg tcaatttaag agaaagagtt acaccgtcac cacttccgtg cactgtataa
     5161 aaatcctata ctgtacgtat cgacagttta gtgagtttta tcatgacggc ggaaggtcac
     5221 cttctctttt ctattgcttg tgcggtattt gccaaaaatg ccgagctgac gcccgtgctg
     5281 gcacagggtg actggtggca tattgtccct tccgcaatcc tgacgtgttt gttaccggac
     5341 atcgatcacc caaagtcgtt tcttgggcag cgattaaaat ggatatcaaa accgatcgcc
     5401 cgcgcttttg ggcatcgtgg ttttacccac agtctgctgg cggtatttgc gctgctggca
     5461 accttttacc ttaaggttcc ggaaggctgg ttcattccgg ctgatgcgct acaaggaatg
     5521 gtgctgggtt atttgagcca catacttgcc gatatgctga cacccgccgg tgttcccctg
     5581 ctctggccat gccgctggcg tttccgcttg cctatcctag ttccccaaaa gggcaaccaa
     5641 ctggaacgtt ttatctgtat ggcattattt gtctggtcgg tatggatgcc ccattcatta
     5701 cccgagaaca gcgctgttcg ttggtcatcg caaataatca ataccttgca gatccagttt
     5761 catcggctta ttaagcatca ggttgaatac taaaaagaca aaaatcacct ttctggaata
     5821 agcaattcca tttgaatata agagccagct cacagttctg ttaatcttgc gccaacacta
     5881 tgactgctac gcagtgatag aaataataag atcaggagaa cggggatgaa ctttccatta
     5941 attgcgaaca tcgtggtgtt cgttgtactg ctgtttgcgc tggctcagac ccgccataaa
     6001 cagtggagtc tggcgaaaaa agtgctggtg ggtctggtga tgggtgtggt ttttggcctt
     6061 gccctgcata ccatttatgg ttctgacagc caggtactta aagattctgt acagtggttt
     6121 aacatcgttg gtaacggcta tgttcaactg ctgcaaatga tcgttatgcc gttagtcttc
     6181 gcctctattc tgagcgcggt tgcccgtctg cataacgcat ctcagttagg caaaatcagt
     6241 tttctgacca tcggtacgct tttgtttacc acgctgattg cggcgctggt cggtgtgctg
     6301 gtcaccaacc tgtttggttt gacggctgaa ggtctggttc agggtggtgc agaaactgca
     6361 cgtctgaacg ccattgaaag taactatgtt ggtaaagtct ctgatctgag cgttccgcag
     6421 ctggtcttgt cctttatccc gaaaaacccg tttgccgatc ttaccggagc caatccgacg
     6481 tcaattatca gcgtggtaat ttttgccgca ttcctcggcg tagctgcatt gaaactgctg
     6541 aaggatgatg cgccgaaagg tgaacgcgtc ttagccgcta tcgataccct acaaagctgg
     6601 gtgatgaaac tggttcgcct tgtcatgcag ttgacccctt acggcgttct ggctctaatg
     6661 accaaagtgg ttgcaggttc taacttgcaa gacatcatca aactgggaag tttcgttgtc
     6721 gcgtcctacc tcggtctgct gattatgttt gcagtgcatg gcattctgct gggcattaat
     6781 ggcgtgagtc cgctgaagta cttccgtaag gtatggcctg tgctgacgtt tgcctttacc
     6841 agccgttcca gtgctgcgtc tatcccactg aatgtggaag cacaaacgcg tcgtctgggc
     6901 gttcctgaat ccatcgccag tttcgccgcc tctttcggtg caaccattgg tcagaacggc
     6961 tgcgccggtt tgtatccggc aatgctggcg gtgatggttg cgcctacggt tggcattaac
     7021 ccgctggacc cgatgtggat tgcgacgctg gtcggtattg ttaccgttag ttccgcaggc
     7081 gttgccggtg tcggtggtgg tgcaactttc gccgcactga ttgtgctgcc tgcgatgggc
     7141 ctgccagtaa ccctggtggc gctgttaatc tccgttgaac cgcttatcga catgggccgt
     7201 acggcgctaa acgttagtgg ctcgatgaca gctggcacgc tgaccagcca gtggctgaag
     7261 caaaccgata aagccattct ggatagcgaa gacgacgccg aactggcaca ccgttaattc
     7321 t
//
LOCUS       DAJVUJ010000185         7184 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.185, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000185 DAJVUJ010000000
VERSION     DAJVUJ010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7184
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.185"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(816..1346)
                     /locus_tag="OQQ48_003700"
                     /pseudo
     CDS             complement(816..1346)
                     /locus_tag="OQQ48_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416483.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
     gene            complement(1689..2339)
                     /gene="zinT"
                     /locus_tag="OQQ48_003701"
     CDS             complement(1689..2339)
                     /gene="zinT"
                     /locus_tag="OQQ48_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HCS1954319.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(2596..3231)
                     /gene="msrQ"
                     /locus_tag="OQQ48_003702"
     CDS             complement(2596..3231)
                     /gene="msrQ"
                     /locus_tag="OQQ48_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HCS1954320.1"
                     /translation="MRLTAKQVTWLKVSLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLRSLFNRLRKQVHNKLSV"
     gene            complement(3232..4236)
                     /gene="msrP"
                     /locus_tag="OQQ48_003703"
     CDS             complement(3232..4236)
                     /gene="msrP"
                     /locus_tag="OQQ48_003703"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HCS1954321.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPAAWQNNLPLTPVDKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            complement(4345..4701)
                     /gene="hiuH"
                     /locus_tag="OQQ48_003704"
     CDS             complement(4345..4701)
                     /gene="hiuH"
                     /locus_tag="OQQ48_003704"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HCS1954322.1"
                     /translation="MVYAAQQNILSVHILNQQTGKPAADVTVTLEKKADNGWLQLNTA
                     KTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLESFFPEIPVEFHINKVNEHYH
                     VPLLLSQYGYSTYRGS"
     gene            4872..5543
                     /gene="hprR"
                     /locus_tag="OQQ48_003705"
     CDS             4872..5543
                     /gene="hprR"
                     /locus_tag="OQQ48_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HCS1954323.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            5543..6901
                     /gene="hprS"
                     /locus_tag="OQQ48_003706"
     CDS             5543..6901
                     /gene="hprS"
                     /locus_tag="OQQ48_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HCS1954324.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHQALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNGYLNIDIASLGTK
                     IHEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSASYHYLNKHNVFRITLPQ
                     RN"
     gene            complement(7009..>7184)
                     /locus_tag="OQQ48_003707"
     CDS             complement(7009..>7184)
                     /locus_tag="OQQ48_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310732.1"
                     /note="Hsp31 stabilizes early unfolding protein
                     intermediates under severe heat stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HCS1954325.1"
                     /translation="LTWYFGEELKKMGMNIINDDITGRVHKDRKLLTGDSPFAANALG
                     KLAAQEMLAAYAG"
BASE COUNT         2016 a         1483 c         1623 g         2062 t
ORIGIN      
        1 aagcgtgctt tcccaatctg gggcaaatca cgtctgaatg acacctgaaa acaggtggtg
       61 gactcaagaa ggcttggaat attcttcttt aatgttatgt aattcattga tttttcgtgt
      121 acgattttaa gacatttttt ccaagaaaaa tttttaactc tttgattttt cgactctgta
      181 tcatcggtct cgaaaaccgg agtaggggca actctaccgg gggggcaaat ccccctctct
      241 ccgccacttt atcaatgact tatctcccga cttcccgcct tacttttcct aaacagaaca
      301 atcgtagaat attcttgaag ggttagatcg tcactgtttt ctgttcgata ctgtgacatt
      361 cagcacttga ttcgctatgg atctgacagg aagctttcga gcgaaaatgt gcagttattc
      421 agtcgttttc ttatcgctca ccattattct tttagacatt catcctatga aactgccgca
      481 aagttggtgg tgggaactga agttgcgtag agaaggggtc aataattgag tcaaaaaagg
      541 aacgcggccg tcagattaaa gccgcgggac aaagtccata atttccaaga gttgttgaat
      601 ttcccagctt ttttgaaagc ctttgtatca tactaacaag taaaaaaggt ttaaatacca
      661 aaattaacct gtatttttga taagtcttac cattgtacga tttacacagt ttctgctcct
      721 ccttgttgat agtggaaagg gtgcattgtg cacttgcgta aacatttatg acgcgttgat
      781 gtccatggtt cacatttcct tgtcagggga tcgacttatt tgcggcgagg catcattcgt
      841 aacagagtat tatctttctg aatgtagtga tgaaagagtg ctgcgccagc gtgagctgcg
      901 attaaaaagt aacctatatt tgcccaggtt tcgtgaatat tttatatcag tgctttaatt
      961 tcgctgtttg gggtaacaaa gggagacaca ttgaaaccaa ggaaactcca cgattttcca
     1021 ctgtaagcca tcaaagcaat ccccagcaga ggaagtgcaa ggaaggttat ataaagcatg
     1081 atgtgcatta gcgaagccgc tttcatttgc caggcaggtg gcggtggcac aatagaagga
     1141 tcactataac ggtgttttat aatcaggcgt gaaaacatta acacccaaac gaatataccc
     1201 gcattgtaat gtgtttctcg catcagcaga taagtactac tacctttagg aaaccagcca
     1261 cggagttcca tcgcggcata ggttattgca acgaaaagca gcgttatcca atgtaaataa
     1321 atttgagttt ttgagaatcg gttcattttt gtagctcctg taatttttac agtcaagcta
     1381 tcccacgaac cttaaaaatt ccttatacag atgtggtttt aagaatttgt tatttaaaaa
     1441 attgatagtg cacattgtaa ccagccaata taagcaatgg cgtgagcaca ggtatctttc
     1501 tctgttggcc gtattgtgta tggaatccgt tattggttaa ctcaatgcgg gttagcttat
     1561 taatatacag acacgtcata gtgatgagcg tgtctgtagc tagcttgcgt tcagtggcgt
     1621 ttgtggatgg accagtggca gttatgatga cttactaaag cggcatcgag gcgttatcat
     1681 gagaatactc aatgagacat catttcctcg accacttctt cgctactcaa ctgatatgga
     1741 taatacgttg gccagttttc catttcattt aataaagatt gctgtgaatc attacccata
     1801 aaaatgtgaa aatgagatga ttttcgtggt gcaattatat gatcgctaaa ttgtatatat
     1861 ttaggggctt tgctttcagg atctttacat tcgaataagt aacgaacgcc tttcttgcct
     1921 gatttatagg tgagtatttt gtatccatcg taatcatatt tacaggatgt tgtttcatta
     1981 tttctatgga attcaacaat gccgtcctca atgccaatca tctcgatatc tgttgcataa
     2041 cctttgtgat aataatcttt aatttcagca aatgttttag ttttatctgc atccgctttc
     2101 ttctgaaaga cggggtcaag tttgccactt tgcagtaaag gataaacgga ttgccagacc
     2161 ccatcccagt cactgagcgt tcggttttgt acattggcat catcaaaaac accattagca
     2221 gctttttgtt cgacctctgt taagggtttg ccgtgtgagt gatgaccatg cgaaaaggca
     2281 ggagcgctaa caataaagac acctaacgca acagccagtt tgtgaagacg aatcgccaaa
     2341 acagtttcct ccagagtcat tgtatatgat gtgtaatgtt atattgtaac atatggcaaa
     2401 atgcaacaac tcttaggaga tgagagcata gttttctcag gtgtacttat ctatcttatt
     2461 gaaataaaat gaattatatt gataatttga ggttgatgac aaacgaaaca taatctgatg
     2521 tgatctgcgt aggccggata gggcgctcgc gccgcatccg gcagggataa cgcgtacttt
     2581 gtcggcaata tgaagttata cagataattt gttgtgaacc tgcttgcgta accggttaaa
     2641 aagggaacgc aacttcttat accgtaaggc taaaagcagt acagccagcc cagcgtagat
     2701 gagcggctgc ggtgaaataa tcttcactga ccacagataa tgtattgggg ccaggatcgc
     2761 gacaagatag acgaaattat gcaactgttg ccagtgcttg ccgagttttc gctgcatcgc
     2821 ctgggttgaa gtaaacgcta aagcaagcaa aataacccag ctgataattc ctaacgttaa
     2881 ataaggccgg gtaattaact ccttgcctaa taacgccaga ttattcacgc ccagctccag
     2941 caatgcgtaa ctggttaaat gcagtgtcgc ccaggcaaag caccataagc ctaacaggcg
     3001 gcgagtgcgt atcaataacg gctgttttgc gtagcgtgcc agaggtgtga ttaacaaggt
     3061 cgccagcaaa aatttcagtg cagtgcgacc ggtaaaatgc tgaatatctt tcaccggatc
     3121 ggcacccagt ccaccgtgat tgatcgccca gaccagccag agaaacggca acaatccggc
     3181 aagatgcagg ctaactttca gccatgtcac ctgttttgct gtcagacgca ttcagaaatt
     3241 ctcccgcaaa tccaggccac gatacagcga tgccacctgg tcggcgtaac cattaaacag
     3301 tagcgttggc tggcgctgaa catcgaggat gccgcctgaa ccaataaatc gttcggtagc
     3361 ctgtgaccag cgcgggtgat caacatgcgg attaacgttg gcgtaaaaac cgtattcgtc
     3421 aggcgctgcc agattccagg tggttggcgg acgctcgcgg gtcagcttaa tactgactat
     3481 cgatttaatc cctttaaagc catatttcca cggcacaatc agtcgtaccg gcgcgccatt
     3541 ttgtggcggt aacgccttgc cataaacacc cacggtcatc agtgtgagcg gatgcattgc
     3601 ttcgtcgaga cgcaatcctt cgacataagg atatttcagc ccgccgccga taaagcggtc
     3661 ctgctggcct ggcatttgtt ccggtgcata aattgtttcg aaagcgacat acttcgcatt
     3721 gctggtgggt tcggcaagcg ccagcaattt gtgcagcgga aaaccaatcc acggcaccac
     3781 catcgaccat gcctccacgc agcgcatacg ataaatacgc tcttccagcg ggaagcgacg
     3841 ggttaaatca tcgtgatcga gggtcaatgg ttttgccact tcgccgctga ttttcagtgt
     3901 ccatggatcg gttttcaggc taccagcatt agcggcggga tcggctttat ccagcccgaa
     3961 ttcatagaag ttgttataac cggagacttt atctactggc gtcagtggca ggttattttg
     4021 ccaggcggca ggcttgctga actccagcgg ttttccggcg ggggcgggcg ggcgatcgtt
     4081 ccctttaaac cagctaagca gatcggcatg cgcagcgtga ggcaaagaaa gtgcagctgc
     4141 actgatgccc agtgctttta acacctgccg acgcttcata aagaataccg actcggccgt
     4201 aacatctgat tcttttaaaa attgattctt tttcataagc catcacactt ttttgtcgtt
     4261 agccagcaag agccgtttac tgttaatctg tcagggagtg tcctccctaa cagtagcaga
     4321 gatctgcttt tacatattta tgcattaact gccacgatag gttgaatacc catattggct
     4381 aagtaataaa ggcacatgat aatgctcgtt cactttatta atatgaaatt caacggggat
     4441 ctcagggaag aaactttcaa gattttgttt cttgaaatag tccccggttt taaatacgac
     4501 acggtaatcg cccgtagttg cagtttgctc gggccacagt gctttaattc gtccatcctt
     4561 atctgttttg gcggtattaa gttgtaacca gccgttatcc gccttctttt caagagtgac
     4621 tgtcacgtca gcagcgggtt ttccggtttg ctggttcaaa atgtgcacgc taagaatgtt
     4681 ttgttgtgcg gcataaacca aagaaggtaa tgaaaatgct gcaaataacg ctttaacatg
     4741 tttatatcct tgtcatgtga atgagttttg aggatagcga aaaagcggcc agggtaacct
     4801 tacagcagca ttacaatttt gtaatgaacc aggctgtttc tataacatat gatttatggc
     4861 atattatttt catgaagatt ttacttattg aagataatca aaggacccag gagtgggtaa
     4921 cgcaggggct ttccgaagcg ggttatgtca ttgatgccgt ttctgatggc agagatgggc
     4981 tttatcttgc gctgaaggat gattatgcat tgataattct ggatattatg cttccgggta
     5041 tggatggctg gcagatctta caaacgttaa gaacagcaaa gcaaacccct gttatttgcc
     5101 ttactgcaag ggattctgtc gatgacagag tcagagggct ggacagtgga gcaaatgatt
     5161 atctggtaaa acctttttca ttttctgagt tgctggcaag ggttcgggca caattaaggc
     5221 aacatcacgc tttgaattca acattagaaa tcagtggctt aagaatggac tctgtaagtc
     5281 aaagtgtgag cagggacaat atcagtatta cactgacgcg caaggagttt cagttacttt
     5341 ggctactggc ctccagagct ggcgaaatta tacccagaac ggttattgcg agtgaaattt
     5401 ggggaatcaa ctttgatagt gataccaata cggtggatgt cgccattcgc aggctccgcg
     5461 caaaagttga tgatcctttt cctgaaaagc taatcgccac aatccggggg atgggctatt
     5521 cattcgtagc ggtaaaaaaa taatgaaaag attgtctata accgtccgtt taaccttgct
     5581 ttttatattg ctgctgtctg ttgctggcgc tggaattgtc tggactctct ataatggcct
     5641 ggcaagtgag ttgaaatggc gcgatgatac aacactcatt aaccggacag cacagatcaa
     5701 gcagttgtta attgatgggg taaatccaga tacgttacct gtatacttta accggatgat
     5761 ggatgttagt caggatatct tgatcattca tggtgatggc atcaataaaa ttgttaaccg
     5821 gacaaatgtc agtgatgaca tgttaaataa catacctgct agtgagacaa tcagcgcagc
     5881 tggcatttac agaagcatta ttaatgatac tgagattgat gctttacgaa ttaatattga
     5941 tgaagtttcg ccatcattaa cggttactgt ggctaaattg gcttcagcta gacataacat
     6001 gcttgaacag tataaaatta atagcattat aatttgcatt gtcgccattg tactttgctc
     6061 agtattaagt ccgctgttaa tcagaacggg attacgagag atcaaaaagt tgagtggtgt
     6121 aacggaagcg ctgaattata acgatagccg agagcctgtt gaggttagcg cattaccgag
     6181 agaactaaaa cctcttgggc aggcgttgaa taaaatgcat caagccttag tcaaagattt
     6241 tgaacgccta agtcaatttg ctgacgatct cgctcatgaa cttagaacgc ccattaatgc
     6301 attactgggt cagaatcagg ttacgctcag tcaaaccaga agtatcgctg aatatcaaaa
     6361 aacaattgcc ggtaacattg aagagttgga aaatatttcg cggttaacag agaacatact
     6421 gtttcttgcc cgggcagata aaaacaatgt tttggtgaaa ctggactcgc tttctctcaa
     6481 taaggaagtc gaaaatttgt tggattatct tgaatatctt tcagacgaga aagagatttg
     6541 ctttaaggtc gagtgcaatc agcaaatctt tgcggataaa attttactgc aacgaatgtt
     6601 atcgaatctt attgttaatg ccattagata ttcaccagaa aaatcgcgta ttcatataac
     6661 cagttttctt gataccaacg gctatcttaa tattgatatc gccagtcttg gaacgaaaat
     6721 acatgagcct gaaaaactct tccgtagatt ttggcgggga gataattcgc gtcattccgt
     6781 aggtcaggga cttggtcttt ctttagtcaa agcgattgcc gaattgcatg ggggaagtgc
     6841 ttcatatcac tatctcaata agcataatgt gttccgaatt acgttaccgc aaagaaatta
     6901 acatagggct tcagtacgcc ataaattaac cgtgaaaaaa cgcagccacg tcgtgagtac
     6961 taacgcggct gcgattaatt atgcgcttac attcaaacgt aacagggatt aacccgcgta
     7021 agctgccagc atttcctgcg ccgccagttt acccaacgca ttcgctgcga aaggactgtc
     7081 gccggtgaga agtttacggt ccttatgtac tcgcccggtg atgtcgtcat taatgatgtt
     7141 catgcccatt ttcttcagct cttcgccgaa gtaccaggtg agat
//
LOCUS       DAJVUJ010000186         7000 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.186, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000186 DAJVUJ010000000
VERSION     DAJVUJ010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7000
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.186"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..376
                     /locus_tag="OQQ48_003708"
     CDS             <1..376
                     /locus_tag="OQQ48_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1954326.1"
                     /translation="RRPNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQ
                     YRKGGLLPSGKNMPALLPVTLTPEPDNKIPAPAQEPEQINTPSDSLCCELVLPAGTLR
                     LKGKLTPALLQILIREIKGSSH"
     gene            376..723
                     /gene="tnpB"
                     /locus_tag="OQQ48_003709"
     CDS             376..723
                     /gene="tnpB"
                     /locus_tag="OQQ48_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCS1954327.1"
                     /translation="MISFPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTRRLERGRFVWPVTRDGKVHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            743..1897
                     /locus_tag="OQQ48_003710"
                     /pseudo
     CDS             743..1897
                     /locus_tag="OQQ48_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            1901..2060
                     /locus_tag="OQQ48_003711"
                     /pseudo
     CDS             1901..2060
                     /locus_tag="OQQ48_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            2649..2947
                     /locus_tag="OQQ48_003712"
                     /pseudo
     CDS             2649..2947
                     /locus_tag="OQQ48_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343747.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3029..3166
                     /locus_tag="OQQ48_003713"
                     /pseudo
     CDS             3029..3166
                     /locus_tag="OQQ48_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            3536..4213
                     /gene="ospD1"
                     /locus_tag="OQQ48_003714"
     CDS             3536..4213
                     /gene="ospD1"
                     /locus_tag="OQQ48_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector/anti-activator
                     OspD1"
                     /protein_id="HCS1954328.1"
                     /translation="MSINNYGLHPANNKNMHLIIGSNTANENKGMKRNIINVTNTAIS
                     HAINEEKSGGGYSGVSFRKLAKIQSISIPTKNNKEYNRHNLFSLIWHGNADAARKYGE
                     SLLAAEIPKEEKLEVLAARNNAGESALFIALQEGHSAAIQAYGDFIKTFDLSPKETIK
                     LLDVRDNEGLPGLFLAAGKGNIEAMMAYINICHHSGIKLTEIADRLNNNEQDMFNIIS
                     DKIQELF"
     gene            4537..4647
                     /locus_tag="OQQ48_003715"
                     /pseudo
     CDS             4537..4647
                     /locus_tag="OQQ48_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            4725..5039
                     /locus_tag="OQQ48_003716"
     CDS             4725..5039
                     /locus_tag="OQQ48_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1954329.1"
                     /translation="MLQRQRGKVGFAQLPVDFVAIEPDSAQGVGKRANLTNRCFIIRI
                     NDSFKKRQGFIEFISNSGSGHTVTVYTKRRFQRGVFMNSLNTNVVKPVMYRLRSSADA
                     RP"
     gene            5321..5887
                     /gene="ipgB2"
                     /locus_tag="OQQ48_003717"
     CDS             5321..5887
                     /gene="ipgB2"
                     /locus_tag="OQQ48_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB2"
                     /protein_id="HCS1954330.1"
                     /translation="MLGTSFNNFGISLSHKRYFSGKVDEIIRCTMGKRIVKISSTKIN
                     TSILSSVSEQIGENITDWKNDEKKVYVSRVVNQCIDKFCAEHSRKIGDNLRKQIFKQV
                     EKDYHISLDINAAQSSINHLVSGSSYFKKKMDELCEGMNRSVKNDTTSNVANLISDQF
                     FEKNVQYIDLKKLRGNMSDYITNLESPF"
     gene            complement(6149..6271)
                     /locus_tag="OQQ48_003718"
                     /pseudo
     CDS             complement(6149..6271)
                     /locus_tag="OQQ48_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            6414..6968
                     /locus_tag="OQQ48_003719"
                     /pseudo
     CDS             6414..6968
                     /locus_tag="OQQ48_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3H family transposase"
BASE COUNT         2050 a         1520 c         1644 g         1786 t
ORIGIN      
        1 ccggcggcct aattttcctt atgaatttaa aatcgcctta gtggagcagt cactgcagcc
       61 cggagcctgt gtggcgcaga tcgcccgtga aaacggaatc aacgataacc tgctcttcaa
      121 ctggcgccat caataccgga aaggtggcct gctgccttcc ggaaaaaata tgcctgcact
      181 gcttcccgtg acgttaacgc cggagccgga taataaaatc ccggctccgg cacaggaacc
      241 agagcagata aatacaccgt ccgacagtct gtgctgtgag ctggttctgc cggccggaac
      301 tctcaggctt aaaggtaaac tgacgccggc gttattacag atacttatcc gcgaaataaa
      361 agggagcagc cactgatgat atctttccct gcaggttcgc gtatctggct ggttgcaggt
      421 atcaccgata tgcgaaatgg ctttaacggc ctggcctcaa aagttcagaa cgtcctgaag
      481 gatgacccgt tctccgggca tctgttcatc ttccgcggac gccggggtga ccagataaaa
      541 gtgttgtggg ctgacagtga cggactgtgc ctctttacca gacgcctgga gcggggccgc
      601 ttcgtctggc cggtcacccg tgacggcaag gtgcacctta ctccggccca gttatccatg
      661 cttcttgagg gcatcgactg gaaacacccg aaacgaacgg aacgcgctgg catccgcata
      721 taacccgttg taaagtgagg atatggacac ctcacttgct catgagaatg cccgcctgcg
      781 ggcactgttg cagacgcaac aggacaccat ccgccagatg gcgaaataca accgcctgct
      841 ctcacagcgg gtggcggctt atgcttccga aatcaaccgg ctgaaggcgc tggttgcgaa
      901 actgcagcgc atgcagttcg gttaaagctc agaaaaactt cgcgcaaaaa ccgaacggca
      961 gatactggaa gcacaggagc gaatcagcgc acttcaggaa gaaatggcgg aaacgctggg
     1021 tgagcaatat gacccggtac tgccatctcc cctgcgccag tcttcagccc gtaaaccgtt
     1081 acctgcctca cttccccgtg aaacccgggt catccgtccg gaagaggaat gctgcccggc
     1141 ctgtggtggc gaactcagtt ctctgggatg tgatgtgtca gagcagctgg agcttatcag
     1201 cagcgccttt aaggttatcg aaacacaacg tccgaaactg gcctgttgcc ggtgtgacca
     1261 tatcgtgcag gcaccggtgc cttcaaaacc cattgcacgc agttatgccg gagcggggct
     1321 tctggcccat gttgtcgctg gaaaatatgc aggctatctg ccgttatacc gccagtcaga
     1381 aatataacgt cgtcagggcg tggagctgag ccgtgccaca ctgggtcgct ggaccggtgc
     1441 cgttgctgaa ctgctggagc cgctgtatga cgtcctgcgc cagtatgtgc tgatgcccgg
     1501 taaagttcat gcggatgata tccccgttcc ggtccaggag ccgggcagtg gtaaaacaag
     1561 aactgcccgg ctgtgggtct atgtccgtga tgaccgtaac gccggttcgg aaatgcctcc
     1621 ggcggtctgg ttcgcgtact caccggaccg gaaaggtatc catccacaga atcatctggc
     1681 cggttacagc ggagtgcttc aggccgatgc ttacggtggt taccgggcgt tgtacgaatc
     1741 cggcagaata acggaagccc tgctgcgtat cggtgaactg tatgccatcg aggcagaagt
     1801 ccggggatgt tcagcagaac agcgtctggc ggcaagaaaa gccagagccg cgccactgat
     1861 gcagtcactg tatgactgga tacagcaaca gatgaaaatt cattcgctga aaatggaatg
     1921 tctccacgga gaacactatt atccatcggg aaatagtgcg ggcaacagtg tttagttata
     1981 tcgagtgcga ttacaatcgc tggcgacggc accgttggtg tggtggtctc agtccggaac
     2041 aatttgaaaa ccagagcctc acttacgact gtgtccatat tatgtgggta ggatcaataa
     2101 agacgtttta tatgtgtctg cttgcagcca gaaaatttat accctgcagg tatcataaac
     2161 atgcttgaca acttaattag ggccccctct tccttttatg gtgtaattgt ttgattgaat
     2221 acttccgggg atttcagtat gaaatgaagt atatttaata tactttacaa aaagtttgta
     2281 tgagtggaat aagaacaata gtttatccaa actgccaagc ccctttatta taagtaagat
     2341 ctggcactgg aatattctca aaaggactaa catcatcacc acgaccttca accaaccata
     2401 aaaaacaata ctattttagg tgatgtatta aattagtaga atatttattc ttctataaaa
     2461 gcactacccc aagaggttaa tcaggtcatc catgattatt tatcatatac taactatgtg
     2521 ggatttcaac gtgaaaactt attttcacta taatcttcag gattcgaaga tcataggagc
     2581 ctgtacataa gtttgtataa ttctctgatg ttgaactatt taatttgata tcaaatgaag
     2641 gtgaatttat gaatgaaaaa cagttgacat ctaataagtt gcgggcttta gctaatgaac
     2701 tagccaaaag tctcaaaaat cctgaagatc tcagtcagtt tgattggatg ctgaaaatga
     2761 aaccgtactc aatgctaata tgactcatca tcttgggtgc gagaaaaatc agcttagatc
     2821 tggcagcaat tcccgcaatg gttgcttaac gaagattatc actactggag atgaacctct
     2881 ggaaatccgt actctgcgtg atcgtaatgg cactttcgaa ccgcaacagc tgaagaaaaa
     2941 ccagccctag attactaaat ctgtcgggcg atggattaac tggttcaggc tgtctggtgc
     3001 tgaaggcctg aagtcactgc tgtcagtgat gtggccggct cttcatgaga caataacccg
     3061 taatcatcag tgccgttcaa tatggccact gttgaaaaaa gttcgtcact ttatggagac
     3121 ggttagccca cttcccggag aaaaacatag tctggccaaa gtgtagcggt attaggctca
     3181 catatttaga tgtagataag ccggagcagg cagttaaatc taggcagcat cacagaaatg
     3241 gacaacgccc cttaaggact ggcgtatggc aatgagctgc tttattatca agtttggtga
     3301 tcgccttgac ggtcacttct gagtgaaggc gcttgcacag aatggtaaac ggactcgatt
     3361 attgaatctt agggataaga tatatctacg atatgtcaat atacgaaatc gaaagaattt
     3421 attggtgaat atagccattc aatgtggcaa tggttttttt agattagcaa tagactaata
     3481 tctattacat tacaaaaata ggttattgtt tttttagcaa aaaggatgaa caaaaatgtc
     3541 aataaataac tatggattac atccagcaaa caacaaaaat atgcacctaa taataggcag
     3601 caatactgct aatgaaaata aaggaatgaa acgtaatatc attaacgtga caaataccgc
     3661 tatatcccac gccatcaatg aagaaaaatc agggggggga tatagtggtg tttctttcag
     3721 aaaattggcc aaaatacaga gcatatccat tccgacaaag aataataagg agtataaccg
     3781 ccataatttg ttttcattga tttggcatgg aaatgctgat gcagcgcgta aatacggtga
     3841 atcgctgttg gcagccgaaa tacccaaaga ggaaaaacta gaagttcttg cagcacgaaa
     3901 taatgctggg gaatctgctt tgttcatagc tcttcaagaa ggtcattccg ctgcgattca
     3961 agcttatgga gattttatta aaacttttga tttatcacca aaagaaacga ttaaactatt
     4021 ggatgtaaga gataatgagg ggttaccagg attatttctg gccgcaggga aagggaatat
     4081 cgaggctatg atggcatata taaatatatg ccatcatagt gggataaaac ttacagaaat
     4141 agcagacaga cttaacaata atgaacaaga catgtttaat attatttctg acaaaataca
     4201 agagttgttt taagtgtgct aaatagctgc aaagaattgc acttagaagg agctctcccc
     4261 gaattgtgtg atctgcttaa tttctaaaaa gaaacaaaca gttaactaga ctgagcgata
     4321 ctacccatag aaccagtttc ccatgacgaa cgacactgga cgcaaaaaca atcataaaaa
     4381 acacaataaa tgtaatgaac acatcccacc ctattaagta aagtgcactg tgatagagga
     4441 agggaaatac aggaaggtgt ggagccttgc agatttctgg catcatagtg cccaatacgg
     4501 aatgcataaa gccccctcag actggagact ccacaaatga aatatacagt gtttaattat
     4561 atcaagtgcg attacaatcg ctgacgacgg cacagttggt gtggaggtct cagtcctgaa
     4621 caatttgaaa accagaacct cacttaagcc tgtgttcata ttacgtgggt aggatcatac
     4681 ttttatagaa gctgtcagtg gctacgtccc agctaaatca cttcatgctc cagcgccaga
     4741 gaggcaaggt tgggtttgct cagctgccgg ttgattttgt tgcgattgaa ccggacagcg
     4801 cccagggtgt cggcaaaaga gcgaatctca ccaatcgttg tttcattatc cgcattaacg
     4861 atagcttcaa gaaacgccaa ggttttatcg aattcatcag caacagtggc tccgggcata
     4921 ccgtgaccgt ttatacaaaa aggcgttttc agcgtggagt tttcatgaat agcctcaaca
     4981 cgaatgtcgt gaagccagtg atgtatcggc tacgatcctc agctgatgcc agaccatagg
     5041 actgacacta cgaacagcaa ttttaatgac gtaaatcttc ataactacaa ctaacagggc
     5101 atcattatga atgatgccgg ttattttacc gctgttggtc ccttcattta gggactgccc
     5161 caagtttttt catactctct ttctcagaga gtttagtctc tgggattcca gacggtccat
     5221 cattgagcca agggagtaaa gaactcaaag catttacaga attgtttgaa tcagctaaaa
     5281 taatgacaat gttactattt tacatgacaa agtgattata atgcttggaa catcttttaa
     5341 taattttgga atcagtttat ctcacaaaag atatttttca ggaaaggttg atgagattat
     5401 ccgctgtaca atggggaaaa ggatagtaaa aatatcatca accaaaataa atactagtat
     5461 tttgagctct gtttcagaac aaatcggtga aaatattaca gattggaaaa atgatgaaaa
     5521 aaaagtctac gtatcccggg tggtaaacca atgtattgat aagttttgtg cagaacacag
     5581 ccgaaaaatc ggcgataatc tccgtaaaca gatatttaaa caggttgaga aagattatca
     5641 catttctcta gacatcaatg ctgcacaaag ttccataaat catcttgtat caggaagcag
     5701 ttatttcaaa aagaaaatgg acgaactttg tgaagggatg aatagaagcg ttaaaaatga
     5761 tacaacaagc aatgttgcaa atcttatttc cgatcagttt tttgagaaaa atgtgcaata
     5821 tattgatcta aagaaattac gtgggaatat gagtgattat attacaaatt tagaatcgcc
     5881 tttctgatga cagaacatcc cgagtaaaga aactaagaaa agtaaatacc caggaattgc
     5941 ctgagcggtg ctatatttct cctcgcaagt aagtcctaat atattgaatt gttgtatagc
     6001 cagtttttgt attttcctag ctcatggatg tgggatccct ctcccctttc tctgctcaaa
     6061 ggtaagcggc tcgtcagaat catattgata tttactgaga agtcagatca actttccaag
     6121 gcaacagatc gcgtacccgg tttgccggtc agtcctggat atgttcaata acgtaacgca
     6181 gccatttttc tggctccaca ttgttcagac ggcatgtgcc gatcagcgag aacaacaccg
     6241 ctgcatgttc gtcaccgctg tcggaacctg cgagtcgagt cagccggaga gagtttcact
     6301 ctccggctgc tcacggaacc gtacgtgaca ctctcgcgtc atacggctct tgatcactca
     6361 ttcgatgcac gtagtaaata ccagtgagca aattgatctt acccagcaat acggtcagtt
     6421 accgcgcaca tggcctgcct gtgtcagaaa atctgctgga gcaggatttt tacgccagtg
     6481 gtccgaacca gaagtgggcg ggagacatca cgtacttacg tacagatgaa ggctggctgt
     6541 atctggctgt ggtcattgac ctgtggtcac gtgccgttat tggctggtcc atgtcaccac
     6601 gcctgacggc gcaactggcc tgcgatgccc tgcagatggc gctgtggcgg cgtaagagac
     6661 cccggaacgt tatcgttcac tcggaccgag gaagtcagta ctgttcagca gattatcagg
     6721 cgctgctgaa gtggcataat ctgcgtggca gtatgagcgc aaagggctgc tgctacgata
     6781 atgcctgcgt ggaaagcttc tttcattcgc tgaaagtgga atgtctccac ggagaacact
     6841 ttatcagccg ggaaataatg cgggcaacag tgtttaatta tatcgaatgt gattacaatc
     6901 gctggcgacg gcacagttgg tgtggcggcc tcagtccgga acaatttgaa aaccagaacc
     6961 tcgcttaggc ctgtgtccat attacgtggg taggatcact
//
LOCUS       DAJVUJ010000187         6952 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.187, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000187 DAJVUJ010000000
VERSION     DAJVUJ010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6952
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.187"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..185
                     /locus_tag="OQQ48_003720"
     CDS             <1..185
                     /locus_tag="OQQ48_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1954331.1"
                     /translation="QKYSAFELAFEFGHRVIAELILNTLNKMAESFGFTDNPRYIAEK
                     NYMEALLKKASPHTVR"
     gene            complement(154..1878)
                     /gene="aceK"
                     /locus_tag="OQQ48_003721"
     CDS             complement(154..1878)
                     /gene="aceK"
                     /locus_tag="OQQ48_003721"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HCS1954332.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     LAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIIRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAALVEWLREILPGKTT
                     AELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRVFKVI
                     KDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMELLLQE
                     AAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAAANIF
                     PGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSVSPGD
                     VFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRRQRFS
                     VRYGEMLF"
     gene            complement(2159..3456)
                     /gene="aceA"
                     /locus_tag="OQQ48_003722"
                     /pseudo
     CDS             complement(2159..3456)
                     /gene="aceA"
                     /locus_tag="OQQ48_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
     gene            complement(3486..5087)
                     /gene="aceB"
                     /locus_tag="OQQ48_003723"
     CDS             complement(3486..5087)
                     /gene="aceB"
                     /locus_tag="OQQ48_003723"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HCS1954333.1"
                     /translation="MTEQATTTDELAFTRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASICDADWKIRGIPADLEDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEEHNNQVLNKVKADKSLEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKMLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFGDAA
                     RLMEQITTSDELIDFLTLPGYCLLA"
     gene            complement(5356..6285)
                     /gene="metA"
                     /locus_tag="OQQ48_003724"
     CDS             complement(5356..6285)
                     /gene="metA"
                     /locus_tag="OQQ48_003724"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HCS1954334.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPEVPYNYFPHNDPQNTPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            6442..6889
                     /locus_tag="OQQ48_003725"
                     /pseudo
     CDS             6442..6889
                     /locus_tag="OQQ48_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
BASE COUNT         1667 a         1789 c         1805 g         1691 t
ORIGIN      
        1 cacaaaaata ctctgccttt gagttggctt ttgaatttgg tcaccgggtt attgctgaat
       61 taatccttaa tacattaaat aagatggctg aaagctttgg ctttacggat aaccctcgat
      121 acattgcgga gaaaaattat atggaagctt tactcaaaaa agcatctccc cataccgtac
      181 gctaaatctt tgcctgcgcc gatacgcata aacatcttcc acatgccctt cacgtatgcg
      241 gttttgtagt gcgcgccagt aatcagcgcg gaacaggtcg gcgtgcatct cttcaaacag
      301 cggaccaata cgcgggtcgg cgcatagcca gtggcgaaac tcttccggga aaacatcgcc
      361 cggcgagacg ctgtaccacg gttcgctggc aagttcgtct tccggatagc gcggcggcgg
      421 gatgtcgcgg aaattcactt ccgtcatgta gcaaatttca tcgtaatcat aaaaaaccac
      481 acgcccgtga cgggtgacac cgaagttttt aaacagcatg tcgccaggaa aaatgttagc
      541 agcggcaagc tggcgaatag cgttaccgta ttcttcaatg gcgtcgcgca actgctgacc
      601 ttccacttgt tccagccaga tattgagcgg caccatccgc cgctcgatat agagatggcg
      661 aattacaatc tgctcaccaa gatcggtgat tttttccgct gcttcctgga gcagtaattc
      721 cattaatgcc ggggaaatat gccgcttctc cagcacaaag ttttcaaact cctgggtgtc
      781 cgccattcgg cccacacggt cgtgctcttt caccagttga tagcaggcac gaacgtgagc
      841 ggcagacatc tctttctgcg gcgcgaactt gtctttgatg actttgaata cccgatcaaa
      901 gcccggcagc gtaaacacca acatcaccat tccacgaata cccggcgctt caatgaactg
      961 ctcattacag ccctgtagat aaacgagata ttcgcggtag ctttcggttt tggcgtgctt
     1021 ctggcagccg atagccatat acaattcagc agtggtttta cctggcagaa tttcccgtag
     1081 ccactcgacc agcgctgcat aaaccataaa ataagaacgc gcaaagccaa aaacaatgct
     1141 cgcttcggcg gtcgtcgtca ggcaggtatc aataaataac tcgccgtcgt ccgtctggtg
     1201 gatcggcagt aagaagggca acgtgccgga aggcgtgatc agtttgccca ccagccaggc
     1261 ggctttattg cggtaaaaca gttcgttcgc cacctgtaaa tgactttccg cgaggttgtc
     1321 tgtccccagc gtttccgtca gatggcgaat gatgtaatga atgtcacggc ttttattctg
     1381 ccagcgcagg cgcagcggca ggtcgctgat aacgcgcatc agtagagatt cccagccgtg
     1441 atcggggtga aagtctttcg ccagcgggcg gggaatggta cgaaagcggc gctctggctg
     1501 agagctaaag ataaaaagcc gctcgggagt aagcgagcgg tggtcaaata accgacagta
     1561 cacggagtta aaaaagctct ccgcaatctc gaagcgcggg taatccggca acagccgcgt
     1621 gtaatgctct ttaacgcgca gtaaaaatgc cgcgtccgta ctttggccgt tagtaatgca
     1681 gcgcagttgc tccacgacca gaccaacgtg atgatcgtaa agatggatac ggtttttcat
     1741 cgccagctgg acagcatgcc agtcggcctg ttcgaaacgc tgctgcgccc cggaagtcac
     1801 ttcgaggaat cgaccatact gagcgtcgaa gccttgcaaa atggtttgag caatcagtaa
     1861 ttccaggcca cgcggcattt agcgccctca tcaggagcag agaattgccg gatgcggcgt
     1921 aaacgcctta tccagcctac gttcggcaac ggttgtaggc atgataagac gcgcaagcgt
     1981 cgcatcaggc atcgtgcacc gattgccgga tgcggcgtaa atgccttatc cggcctacgt
     2041 tcggcaacgg ctgtaggcat gataagacgc gcaagcgtcg catcaggcat cgtgcaccga
     2101 ttgccggatg cggcgtgaac gccttatccg gcctacagtc agcaacggtt gttgttgctt
     2161 agaactgcga ttcttcagtg gagccggtca gcgcggtgac tgaagacgtg ccgccctgaa
     2221 taatagtcgt cactttatcg aagtaacctg tacccacttc ctgctggtga gatacgaagg
     2281 tatagccatc tttcgcggcg gcaaattccg gctgctgcac tttctcaacg tagtgcttca
     2341 taccctcgcc ctggtcatag gcgtttgcca ggtcaaacat gttgaaccac atgctgtgga
     2401 tacctgccag ggtgatgaac tggaacttgt agcccatatc cgacagctgc tgctggaagc
     2461 tggcaatagt tttgtcgtcg aggtttttct gccagttgaa cgacggcgag cagttataag
     2521 ccagcagttt gcccggatat ttcgcgtgga tagcttgtgc aaagcgacgc gccagttcca
     2581 gatccggcgt ggaggtttca caccagacca ggtcagcata tggcgcatac gccaggccac
     2641 ggctgatcgc ttgctcaatg cccgcatgag tacggaagaa gccttcactg gtacgctcgc
     2701 cggtaataaa ttcgctgtca tacgggtcgc aatcggaggt gatcagatcc gcggcatcgg
     2761 tacgagcaac cagtagggtt ggaacgcccg tcacgtcagc tgccagacgc gccgcgacca
     2821 gtttctgaat agcttcttga gttggcacta aaactttgcc gcccatgtga ccgcatttct
     2881 tcactgacgc cagctgatct tcgaagtgaa ctgccgctgc accggcttca atcatcgctt
     2941 tcatcagttc aaaggcattc aggacaccgc caaaaccggc ttccgcatcg gcaacgatcg
     3001 gcaggaagta atcgacatag cgcggatcgc ccggctcaat gcccgcggac cattggatct
     3061 gatccgcacg gcggaaagta ttgttgatcc gctccaccac agctggcacc gagtttgccg
     3121 gatagagcga ctgatccgga tacatgctgg ccgccaggtt agcgtccgcc gctacctgcc
     3181 atcccgacag atagactgct tcaatacccg ctttcgcctg ttgcagcgcc tgaccgccag
     3241 tcagtgcacc gaggctgttg atgtagcttt tttcgactcg ccatgcagca gacgccacat
     3301 tctcgccgcg cccagttgcg ccagggtgca ttcaggattg actgaatcgc gtaatttcac
     3361 cacatcttcc gcgctgtatg ggcgagtaat gccttcccaa cgcggttgag tccactcttt
     3421 ctgtaattct tcaatttgtt gtgtacgggt tttcatgtgc agatgctcca tagttatgtg
     3481 gtggtttacg ctaacaggca gtagcctggc agggtcagga aatcaattaa ctcatcggaa
     3541 gtggtgatct gttccatcaa gcgtgcggca tcgccaaaac gcccctggga gaaacgttct
     3601 tcgcccagtt cgctggcaat gactttcatc tcttcgccca gcatctggcg gaacaaggct
     3661 ttagttaccg gtttgccatt gctcaacatt ttttgatgat ggatccactg ccagattgag
     3721 gtacgggaaa tttcagccgt cgccgcatct tccatcaggc cataaatcgg cacacagccg
     3781 ttgccagaga tccacgcttc gatgtactgc acggctacgc gaatatttgc gcgcatacct
     3841 tcttcggtgc gttcaccgtc acaaggtgcc agtagctgat cggcagtaat cggcgcgtct
     3901 tgttcgcgca tcacttcaag ctgattttta cgggagccga gaatgtcgtt gaataccgcc
     3961 attgccgtat ccgcaaggcc tgggtgagcg atccatgtgc catcgtgacc gttattggct
     4021 tccagcgatt tatccgcttt tactttgttg agcacctggt tattgtgctc ttcatctttg
     4081 ctcggaataa acgccgccat gccgcccatc gcaaaagcac cgcgtttatg gcaggtttta
     4141 atcaacaggc gtgagtaagc attcaggaat ggtttatcca tcgtcactgc ctgtctgtct
     4201 ggcaggacgc gatcgggata gtttttcaac gttttgatat agctgaagat gtaatcccaa
     4261 cgaccgcagt tcagaccaac aatatggtca cgcagcgcgt gaaggatttc atccatctgg
     4321 aacacggcgg gcagcgtttc aatcagcaac gtcgccttga tggtgccgcg cggcagatta
     4381 aagcgatctt ctgcatagct gaagacttcg ctccaccagg ccgcttcctg ccaggactgg
     4441 gttttcggca gatagaaata gggaccactg ccctttgcca acagagcctg atagttgtgg
     4501 aagaaataga gcgcaaaatc aaacaggctg ccggggattg cctcaccacg ccaggtgaca
     4561 tgtttttccg gcaagtgcag accgcgtacc cgacaaatca aaaccgctgg attgggcttg
     4621 agctggtaaa ttttgcctgc ttcattggta tagctgatgg taccgttaac tgcatcacgc
     4681 aggttaattt gcccgtcgat cactttgttc cagtccgggg ccagtgaatc ttcgaaatcg
     4741 gccataaaga ctttcacatt ggcgttgagc gcgttgatca ccatcttgcg ctctaccggg
     4801 ccagttatct ctacgcggcg gtcttctaag tccgcaggaa tcccgcgaat tttccaatca
     4861 gcatcgcaaa tggaagctgt ttccgaaata aaatcaggca acgttccgtt atcaatatct
     4921 tgctgctgct gaatgcgcgc tgccagaagt ttatttcgtt gtggcgtaaa atgcgtcacc
     4981 agctcagtca gaaattctac cgcttcggca gtaagaattt gcttctcctg ctcgccatac
     5041 ggccttgtga aagccagttc atcggttgtt gttgcctgtt cagtcatcgt gcagctcctc
     5101 gtcatggatc cgaaaacttc cccactgtga acgaaggatc gttgtgcgct tttcgttcag
     5161 cacaactaag actactcatt taaaatgcaa aatcaaaaac aatttccatt ttaattgaaa
     5221 atgcttgata acttattgat aacaatgtga ataaaattta ttcacctcct gaggtgcgtt
     5281 tcggaaataa aaaaggcacc cgaaggtgcc tgaggtaagg tgctgaatcg cttaacgatc
     5341 gactatcaca gaagattaat ccagcgttgg attcatgtgc cgtagatcgt atggcgtgat
     5401 ctgatagacg taatagttga gccagttggt aaacagcaaa ttaccgtgac tacgccagct
     5461 cgctcgcggt gtattttgcg gatcattgtg cgggaaatag ttatacggta cttccgggtc
     5521 tagtccggct tccacatcgc ggaaatattc ctgcgccagc gtttgcgcat catattcggg
     5581 atggcccgtc acaaaggcaa tgcgcttatc tttgctggca aacagatatg catccccttc
     5641 ttccgtctct gccagaattt ccagatcggt gtaatcacga atcaacgctg ccggaaagtc
     5701 agcatagcgc gaatgtggtg ccaggaatga atcatcaaag ccacgcgtca gaagcgcatg
     5761 aggatggaga atatgatgct cgtaaacgcc agagagtttg tcggtgcgag tttgcttagg
     5821 aatgccgtag aggatattga gcgcggcctg taccgcccag cagacaaaca gcgtcgaggt
     5881 gacgtgatct ttcgaccact ccagcacctg tttgatctgc ggccagtaag cgacatcatt
     5941 aaactccacc aggcccagcg gcgcaccggt tacaatcaga ccgtcaaagt tctgttcctg
     6001 aatatcttca aagttacagt agaagttgtt cagatgctct gcgggcgtgt tgcgcgattc
     6061 acgggaatcg atgcgcaaca gctgaatatc gacctgcaaa ggtgagtttg aaagcaggcg
     6121 cagaaactga ttttcagttt caatcttctt cggcatcagg ttaaggatca gaaccttaag
     6181 tggacgaatt tcctgaccag acgcacgaga agttgtcatc acaaagacgt tttcttcacg
     6241 caagaaattg acggcgggta gctcgtccgg cacacgaatc ggcataacct gattacctca
     6301 ctacatacac ttatacgttt agacatccag atagctgaag ataaccagaa aatttgccaa
     6361 tgtcgagcct gcatgttgaa ggtgagaaag tttcaaggaa aagactacgt tagaatataa
     6421 gaatgactaa aaggagagaa aatggttatt agtattcgcc gctcacggca tgaggaaggg
     6481 gaggaactcg ttgcgatttg gtgtcgatct gtcgatgcca cacacgattt tttatcagca
     6541 gagtatcggg ccgagctgga ggtgctagct agttcgttcc ttcctgccgg aagcgccgtt
     6601 gtggatcgcg gttaatgaac gggatcagcc ggttgggttt atgttgttaa gtgggcagca
     6661 tatggatgcg ctgtttatcg atcctgatgt gtgcggctgc ggcgtggatc ggatgctggt
     6721 ggagcatgcg ctctcgatgg caccggaact gacaaccaac gttaatgagc aaaatgagca
     6781 ggcggttggg ttctataaga aggtgggttt taaggttacg ggacgctctg aggtggacga
     6841 tttggggaaa ccgtatccgt tgctgaatct ggcgtatgtg ggggaataag ccgcccctgc
     6901 cgtgcctgcc caggctggag aagacgcgcc agcgtcgcac ccggcatttt tt
//
LOCUS       DAJVUJ010000188         6874 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.188, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000188 DAJVUJ010000000
VERSION     DAJVUJ010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6874
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.188"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(<1..1001)
                     /gene="arnT"
                     /locus_tag="OQQ48_003726"
     CDS             complement(<1..1001)
                     /gene="arnT"
                     /locus_tag="OQQ48_003726"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HCS1954335.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFASLAMLMAHYALLA
                     "
     gene            complement(1001..1891)
                     /locus_tag="OQQ48_003727"
     CDS             complement(1001..1891)
                     /locus_tag="OQQ48_003727"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HCS1954336.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKTEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            complement(1888..3870)
                     /gene="arnA"
                     /locus_tag="OQQ48_003728"
     CDS             complement(1888..3870)
                     /gene="arnA"
                     /locus_tag="OQQ48_003728"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HCS1954337.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VAHLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHATRQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISIAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            complement(3870..4838)
                     /gene="arnC"
                     /locus_tag="OQQ48_003729"
     CDS             complement(3870..4838)
                     /gene="arnC"
                     /locus_tag="OQQ48_003729"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HCS1954338.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            complement(4842..5981)
                     /gene="arnB"
                     /locus_tag="OQQ48_003730"
     CDS             complement(4842..5981)
                     /gene="arnB"
                     /locus_tag="OQQ48_003730"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HCS1954339.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            6232..>6874
                     /locus_tag="OQQ48_003731"
     CDS             6232..>6874
                     /locus_tag="OQQ48_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCS1954340.1"
                     /translation="MSLNFTVIVWLRLSIGGVYELAFCRSSLKSKKYFIILLALAAIA
                     GLGTHAAWSSNGLPRIDNKTLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKG
                     TQDARELGNAFSADIPDFDLYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAI
                     KDLQSKAPDKNIVIFTHNHCLTYIAKNKRDATFKPDYLDGLVMHVEKGKVYLDG"
BASE COUNT         1759 a         1735 c         1701 g         1679 t
ORIGIN      
        1 gccagcaaag cgtaatgcgc catcagcatt gccagagatg caaagcagga aagaatatag
       61 gtgggcaatt tacctttagc gacggagaaa aacagcagcg gcattatcgt ccagctcaac
      121 aaatagacgg ttgcggaatg cttgcggttt ttccagcctg tgtacagtgc accggggagt
      181 aatcccagcc acggcaggct accggcaatg atgaccggca cgtagtacca gaacggagct
      241 ctatgttggg catcatccag tgcaaagcgt tgaatatgct caacccagaa aaaatagtgc
      301 cagaagtcag gctcccgctg cgctatcgcc agtccccaag ggagaaccgt cagtacgcaa
      361 ctgataaccg ccagccagcc gtaaataaag agatctttcc agcgtttttg cgtcgctacc
      421 catggcagca cgcttaatac cggcacggca agggcgagaa aacctttggt catcaccccc
      481 atgccgcagg tgattcccag cagtaaaaat cctgcgcttt tgcctttcca cgtctgtgcc
      541 tgcattgcca gccagaagct gcacattccc gccaccagcc agaatgcaat aaacggatcg
      601 agcacggcat aagtgccgat ggcatagaca ataaacaatg agagataaat tactgtggcg
      661 agtagagcca gacgtttatc gcgccataag cgcagagtaa accaggtcac cagcgcggca
      721 gttaacaggg tcgcaaagat aacgcctgcc cgcacaccaa agttattcgc gccaaatagc
      781 cattgcccaa tgctgttaat ccagtatccg gcaatgggtt tttcgaaata acgtagccct
      841 aacagatggg gcacaatcca gtcgccggat gccagcattt ctcgactgat ttccgcataa
      901 cgcgtttcat cgggttgcca gagcagacgc gtgctgatcg gtaacaggta atagcaggca
      961 ataaacgcga agaggccgat aaggtaacgt accgatttca tcagcgacta cccgcaattt
     1021 gttggcaacc cagccagcct tcacgtccgg caatatttcc gcgaacaact tgtccgagcg
     1081 gcaacgtctc tgataacagt tcgcttaaag ggcaaaatgt cacgccttcc tgagctgcgc
     1141 gtttgaggag atccacaaaa ttatgctgat aagcgcagcc ttcaacttct gcatgaatgg
     1201 tataaaccgg cgtgccttta tctcgcagga tgcggttgag taaccaaccg ttaaaatctt
     1261 ctgttttcac atcccgacca atcacttcat cccaggtggg taaggtcacc ggaatttgcg
     1321 cagtgccagg gtttcctgat tcgagcaatg gacggaacgg catggcccca cgacaatcgc
     1381 tgttgtagcg caaatggaac gcttcttttg cttcgatcac cttctggtcg gcacgccagc
     1441 ccgcagcggc agaacaggtt accggttgac cgataatctc ttccagagtg cgaagaccgc
     1501 gtgcaatatc gtcgatcatt gtttgccgat cccagttacc gctacgggct tgccaggcat
     1561 ggtgatccca ggcgtgcagg ccgacttcgt gatgtttagc cgcttcacga atgatatcgg
     1621 cattggcatg accaatctct ttacctggcc aggccgtacc tgccagtaaa atatcccagc
     1681 cataaagcga tgccgcgttt gagcgcagca tcttccacaa aaactgtggc ttcaccagtc
     1741 gccagagatg gcggcccata ttgtccgggc cgacgctgaa aaaaatgctg gcctgaatat
     1801 tatgcttact caagatttcc agcagacgcg gcacgccttc acgggtgcca cgaaaggtat
     1861 cgacatcaat gcgtaagcct actttggtca tgatggttta tccgtaagat caacggtgcg
     1921 caggaagaaa tccagcgttt cgtcgatggt ttcctgcata tcaattttcg gctcccagtc
     1981 caggcagcgg tgggcattgc ggatgctcgg tttacgatgc tctacgtcct gatatccttt
     2041 gccgtagtag ctgctacttt cgacaacgcg aaagcccgca aacggtggga aatgatggcg
     2101 cagcggatgt ttttcgaagc tcgccagcag catctcgccc agttcctcaa tgctcgcttc
     2161 gttctcagga ttgccaatgt tgataatttc accgtcgcag cgatttcccg cattttcgat
     2221 aatgcggtat aacgcctcga taccatcgcg aatatcagta aagcagcgtt tttgttttcc
     2281 gccatcaatc agcttaatcg gtgaaccttc taccagattg agaatgagtt gcgtaatagc
     2341 gcgggagctg ccgattcgtg ccgcattaag gttatccagt cgtggtccca tccagttaaa
     2401 cgggcggaag agggtgaact gtaaaccctc tttttcgcca taggcccaga tcacccgatc
     2461 aagtaattgt tttgataccg aataaatcca gcgtggttta ttcaccgggc cgacgattaa
     2521 attagaatgg tcctcatcga agtatttatc gctacacatc ccataaactt ctgaagttga
     2581 cgggaagatg attcgcttac ggtacttcac gcagtagcgg ataatgcgca gattctcttc
     2641 aaaatcgagt tcaaatacgc gcagcgggtt gcgggtatat tcaatcggcg tggctatcgc
     2701 caccagcggc aagacgacat cacatttttt gacatgatac tcaatccatt cggaatgaat
     2761 actgatatcg ccttcaacaa agtgaaaatg cggatgattc agaaaacggc ttatcgcatc
     2821 gctgccaata tccagaccgt aaacttcata atgatcttcg cgcagcaggc gttctgtcag
     2881 atggttgcca ataaagccat tcaccccgag gatgagtacc cgggtacggc gtcgggcggt
     2941 gcaggcaggc tggctattca agcgtgaacc ttgcaccagg cccagcgtct gcgctaattg
     3001 cgagccctgc atagtaatgc cgtcgcccgc ctgtccggtg acgatttcca gcgcgccatc
     3061 gccacaggca atcaacagcg gtgcaataga aatcacgctc cccggctgtg ctttgctggc
     3121 atgaggatga acacgcgacg accagacggt gaatttctga ttgccaacat agctgaaggc
     3181 acccggccac ggatcggcaa cggcacgtac catgttgtgc agtacggatg ccggtttatg
     3241 ccattcaagg aaactgtcat ccggcgttct gcgaccaaaa caggtggctt cgttttcgcg
     3301 ctgggcgatt tccagaatat taccgtgttt aatggcgggt aatgtctgtt ccagtagctg
     3361 gcgcgtggca tggcacaatt tatgatgcag cgtaatagcg atatcgtctg gcgcaatggc
     3421 aacgcgcagt tgcgccacaa tggccccagc atcggcacgt tttaccattc ggtgcaatgt
     3481 aacgcccgtt tccgtttcac cgttcaccag cacccagttc agcggcgcgc gaccacgata
     3541 ttttggtaac agcgaaccgt gcagattaaa tgcacctgcg ggagcgagct gcaaaatctc
     3601 gtcgcaaata agatggcgat aataaaaaga gaaaatcacc tctggtgaca gttgggcaat
     3661 gcgttccacc cacagcggat gattaacgtt atccggcgca taaaccggaa tgcctctttc
     3721 cgccgccaga tgagccaccg aaccataaaa ggctttttca ccgggattat cggtatgggt
     3781 aaaaatggcg ctaatttcgt aaccggcagc cagcagagct tcaataccga ggcatcccat
     3841 atcgtggtag gcaaaaacga cggttttcat tattcatttt ccttgctgga tggacggata
     3901 acttgctgaa caaaatagcg ggggcgggcg cggacatcgg tgtagatcct gccgatatat
     3961 tcaccgagta atcccatgcc gataaactga gcgccaataa aagtaaacag cacggcaaat
     4021 agcataaaga cgccttctgc cgcccattgt ggtccgaagg tcaggcgtaa aatcaccagc
     4081 agcaccgcaa tgctaaaacc tccaatcgca ataatgctgc cgagcagact cagcatacgt
     4141 agcggcgttg tggtaaggca ggtcaccagg tcgtacatca aattaatcag gcgcataaaa
     4201 ctgtatttgg attcaccaaa ctcacgctcg gcatgatgta ctggaatttc aatggcacgg
     4261 cgggcgaaga tattcgccag aatcgggata aaggtgctac gttcatggca gtgcaacatc
     4321 gcatcgacaa tatgacggcg ataggcgcgc agcatacaac cgtagtcacc catcgctttg
     4381 ccggtggtgc gctgaataag ccggttaatc atcttcgaag ctgttttacg aaaccagctg
     4441 tcctggcggt tctggcgtac agtccctacc acgtcgtaac cttcatcggc ttttgccact
     4501 aggcggggga tttcttctgg cggattctgg agatcggcat caagggtaat aattaagtcg
     4561 ccagtaacgt gactgaatcc cgccataatc gctgaatgtt gcccgtaatt gcggttaagc
     4621 aaaatagaca caatatggct gttctccgct tgtgaggctt cgaccagcat atgcgcggaa
     4681 ttatcgctac tgccgtcatc aatcagcagg atctcatact ctttccccaa cgattcacag
     4741 gctgtggtgg tgcgcctgat taattccggt aagctttcct gctcgttata aacgggaata
     4801 accaccgaga ctttcttaac agggtggatt tcaaacatgg cttattgtcc tgcgagttgc
     4861 tgaagggctg tgatgacacg gtcggcatcg gcggtagtca tatccgggaa cagcggcaaa
     4921 gaacagatgc gttcgctatt ccattcggta ttcggtaacg acagcgtggg aaaacgctcg
     4981 cgataatatt tttgtgtgtg agcggcgcgg aaatgtaacc cggtaccaat gcctctttct
     5041 tttaacgctt ccatcaacgc atcgcgactg ataccacaac gttgttcatc gacacgaata
     5101 ataaacagat gccaggcgtg aacgtgcggc caggcgggaa ggcttaatgg ctgaaagggg
     5161 agagcagcca gtgcttgctg atattgctgg gcaatttcgc gccgacgggt gttgaggtgc
     5221 tctaattttg ctaactgtgt cagggcaatt gcggcgttaa tatcggtcag attgtactta
     5281 tagcccggtg ttaagacttc agcctgcggt gctcggcccc aggtttgtct gtcataggca
     5341 tcgacaccca ggccgtgaaa tttcagcatc cgtagctggc gggcaaggtt ttcattatca
     5401 gttacaatca ggccaccttc cgcacaggta atatttttaa tggcatgaaa tgaaaaaata
     5461 gcggtacctt ttgcgccaat atgtcgccct ttgtaatacg taccgacggc atgggcagca
     5521 tcttcgataa ctgcgatgcc gtaacgttcg ccaatggcgc gaatggcgtc aatatctgct
     5581 ggcgcaccgg cataatgcac cggaatgatg gctttagtgc gtggcgtaat ggctgactcg
     5641 atcgcttcag gcgtgaccat cagcgtatcg cggtcgacat ccaccattac cggcgttgca
     5701 cccaacaagg aaatcatatt gagggttgaa acccaggtca gggaaggcgt aatcacttca
     5761 tcgcccttgc caatttccaa cgccattagc gtgatatgca ttccggcggt ggctgaactg
     5821 accgcgatgg catgctgatt tcccgtcaac tggcaaaagg cttgctcaag cgcctgattc
     5881 ttcggaccgg ttgtgatcca accggattcg agaacttctt taactgcagc gagttcctcc
     5941 acgcccattg ctggtcgcga aaaaggcaaa aattctgaca ttgcttttcc ttccgccatt
     6001 gaatacctgt ccacttatat ttgctataaa gagtgttgtg tatattttgc catttggaac
     6061 gaaattttaa ggatagaata ttaacttaac cttaagaaac taatattaga cgtaaatatt
     6121 gaaattttta tattttttct tatttaggct ttgcatttgg caaaattttg aggcattttg
     6181 ccgacatcgt aggattttta atattacacc aactgctaat tatcgccaga aatgtcgctc
     6241 aatttcacgg taattgtctg gttgcgcttg tctataggtg gagtttacga gttagctttt
     6301 tgccgctcat cattgaagtc aaaaaaatat ttcatcattt tactggcgct cgctgcaatt
     6361 gccggactgg gtactcatgc cgcctggagt agcaatggtt tgccacgtat cgacaataaa
     6421 acattggcca gactggcaca gcagcacccg gttgtcgttt tgtttcgcca tgctgaacgt
     6481 tgcgatcgtt caaccaatca atgcttgtca gataaaacag gtattacggt taaaggtacc
     6541 caggatgccc gtgaactggg caacgctttt agcgctgaca tccctgattt cgatctttat
     6601 tccagtaata ccgtccggac cattcagtcg gcgacctggt tttccgcggg taaaaagctg
     6661 acggtagata aacgacttct tcagtgcggt aatgagattt atagtgcaat taaggactta
     6721 caaagcaaag cgcctgataa aaatatcgtt attttcaccc ataatcattg cctgacatat
     6781 attgctaaaa ataagcgtga tgcgacattt aaacctgatt atctggatgg tttagtcatg
     6841 catgtggaaa aaggcaaagt ttatctggat gggg
//
LOCUS       DAJVUJ010000189         6854 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.189, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000189 DAJVUJ010000000
VERSION     DAJVUJ010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6854
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.189"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(<1..311)
                     /locus_tag="OQQ48_003732"
     CDS             complement(<1..311)
                     /locus_tag="OQQ48_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HCS1954341.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     V"
     gene            complement(305..1543)
                     /gene="preT"
                     /locus_tag="OQQ48_003733"
     CDS             complement(305..1543)
                     /gene="preT"
                     /locus_tag="OQQ48_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HCS1954342.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAQPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEVGNTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRARQAQGDISIPQSALIIGGGDVTMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFTSARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MAADKIILAVGQHARLDAFAELEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTMVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(1940..2179)
                     /locus_tag="OQQ48_003734"
     CDS             complement(1940..2179)
                     /locus_tag="OQQ48_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HCS1954343.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(2182..2901)
                     /gene="sanA"
                     /locus_tag="OQQ48_003735"
     CDS             complement(2182..2901)
                     /gene="sanA"
                     /locus_tag="OQQ48_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HCS1954344.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(3051..3935)
                     /gene="cdd"
                     /locus_tag="OQQ48_003736"
     CDS             complement(3051..3935)
                     /gene="cdd"
                     /locus_tag="OQQ48_003736"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCS1954345.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(4065..4760)
                     /locus_tag="OQQ48_003737"
     CDS             complement(4065..4760)
                     /locus_tag="OQQ48_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HCS1954346.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMHIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(4757..5155)
                     /locus_tag="OQQ48_003738"
     CDS             complement(4757..5155)
                     /locus_tag="OQQ48_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HCS1954347.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            5394..6344
                     /gene="dusC"
                     /locus_tag="OQQ48_003739"
                     /pseudo
     CDS             5394..6344
                     /gene="dusC"
                     /locus_tag="OQQ48_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311053.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
     gene            complement(6732..6815)
                     /gene="yohP"
                     /locus_tag="OQQ48_003740"
     CDS             complement(6732..6815)
                     /gene="yohP"
                     /locus_tag="OQQ48_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HCS1954348.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
BASE COUNT         1775 a         1759 c         1706 g         1614 t
ORIGIN      
        1 aatactttgt ccgggtaatc ttctttcagc cgacgcaggg cggccagatt ctcctccagc
       61 ggatgctcgg caatctgctc catatttttg aagccgataa aaccggtatc ttctttcacc
      121 agatgatcaa aacgcggcga gacttcgttg gcgataaaaa agccgatcgt tttaaacacc
      181 acaccgcccc aacctgtgtc gtaggctttg gcacacatct catagcagtt gcctaccggc
      241 gaagaagaga ggcagaacgg gttgggaaac ttcacgccgc aaaaagtaat cgaaagatct
      301 ttcgttaaca tgagcaagct ccctctaaat agtgatgaat cgcttcggcg gcttctttcc
      361 cggttttcac ggcatagacc atggttttgt caccctcaac aatatcgcca gcagcaaaga
      421 cttgcgggtc gcgggtctgg taattttgtg ttttgatggt gttacgctgc ggctctaact
      481 ccgcaaaggc atctagtctg gcatgctgac cgacggcgag aataatttta tctgccgcca
      541 tcgtcagttc gcccgataac cgtacatgct taaacgtgac tttattgcct tccacggcta
      601 ctggcgtgaa tccatcgatg atcgaaacgc ccagctcccg ggcgctggta aactcttttt
      661 cgcttgccgg aaactcatct aactcttcac gcgctacgca agttaccgcc tgacagccga
      721 gaactttcag cgtgctggct acgtccatcg tgacatcacc gccgccgata attaatgcgc
      781 tttgtggaat gctgatatcg ccttgcgcct gtcgtgcacg ttgcaagaag tcgacggcaa
      841 tctcaacgtc actatgctca aacagcggta gcccggaacc gcttgataac cccacggtga
      901 ccagtaccgc gcggttttct gctttcagct gctcaagggt gagtgtgtta ccgacttcgt
      961 tgttgcactt aatggtcacg cccatttttt caatacgggc gatctctgca tccagtactg
     1021 attgcggtaa ccggaattgc ggaatgccgt tacgcaacca gccaccgggt tgtgcttctt
     1081 tctcatagat cgtgacgtca taaccctggt ttgtcagtgt cacactggcc tgcaatccgg
     1141 caggacctgc gccaataatc gcgactttgc cgagcgtttt agtaccgggc tgataaattt
     1201 ccatcccggt ttgttgttca aaatcagtaa caaaacgctg taagcggccg atatcaatgg
     1261 gagcatcaac accggcacgg gtacaaccgc tttggcataa tttctccgtc gggcacactc
     1321 tggcacaaac ggcaccgagg gcattatttt cgcgaattgt ctcggcagcg cctttaaaat
     1381 tacgaaagta gattgagcga ataaatttcc ccggatcggt ctgtgccggg caagcctgac
     1441 tacagggagc gtcgtgacat aataaacagc gagaggcttc tttaatcgcc agtaacggcg
     1501 taaaagccgg agtgagttca tccagataat tttgttgcgg catgtgatct tccttaatgc
     1561 tatgagtaat taaggcgaac gctaaatata ctcgtcatac ttcaagttgc atgtgctgcg
     1621 tctgcgggtt attcggccca tccatgggcc tcacccctta tggggccgct gcaggcagcg
     1681 ttcaaatccg ctccatgcgg atttgtcgct caccccagtc acttacttat gtaagctcct
     1741 ggggactcac tcacttgtcg ccttcctgca actcgaatta tttagagtat aaatattgcg
     1801 tgctgatatt aaggctatgc gcgtggggaa atttatactg tgaaaggatt cacaaatagc
     1861 tgcggttcag aatgttatat ccgcagctta ttgagtgaag taataattat tctcaataat
     1921 taacagaaaa gagaaaatat taacgataga aaataagata aacaatcatg ccgatagata
     1981 aaattccgaa gccgatatat gccaccatat aagcgaaaaa gagtccggga ttttttgctc
     2041 gccagacata caccggttcc ggtgcatttt taacccgttt atcaatcgcg cctgcgcgcc
     2101 agcctttcca tgcttcgcgg aaacaaaata tcggaataag gaaaaaaacg gcaacgacaa
     2161 aaaactgctg tacatccata actactttcc ttgtttcttt tgtaattcaa gtaactgttc
     2221 gggtgtgacg gcgggatacc cctgcgcatc ttccggcacc tggtgcatag ccggaatagg
     2281 gaccagcggc cctaaaaaac gcggttcacg tttaaaaata taaaggtcag ccagcgcacc
     2341 gaaacgggcg gcaaattcgc gaatacgtac tgacagcata tctttcggtg acggtacggc
     2401 ataacactga gcctgaatcc ccatatgcag cgcaataaat aatgcccgct cacagtggaa
     2461 acgttgggtg ataataatga aatcattagt atcgaaaact ttgcgtgtac gcacgatgga
     2521 atccagcgta cgaaagcctg cgtaatcgag aacaatatct gatgggtcga caccagcagc
     2581 gattaaatct ttgcgcatgg tcatcggctc attataactt tgcaatgcgt tatcgccgct
     2641 cagtaataga taatttacct taccgctgtt ataggcatta atcgctcctt gaatgcggta
     2701 gcgataatac tgattaatta cgccagtacg ataatatttt gctgttccga gcaccacacc
     2761 gacctggcgg taggggagat cctgcaattc gtcgtagata taaggcgcgg ttttccagct
     2821 catccagcga tcgaggccga gcacagtcaa caacagcaag ccgatcagga ctaacaggct
     2881 gaggaacacg cgctttaaca tgcaggagac tcgatctaag gaaaatttcc atcaggctac
     2941 tttacccgct ggttttgcgc aagaaacggc aaactgtttg ccgggaattt cgacaccagg
     3001 agagggctgg caagccggac aaggcgttca cgccgcatcc ggcaccaggc ttaagcgaga
     3061 agcactcggt cgatactgtg acagccgaga gctttcagcg ttgcggaggt ggcatcccac
     3121 tgaatcaacg gcgcatcggc tttttctgcc agaaccgcgc gctggatatc cgggtaatca
     3181 taacccttga gattcaacag aattaacgct ccctgcaacg gtggcagagt cgggttgaat
     3241 gcggcgtttt cagcgtagct gccactgaaa atacgaccgt ctttacattc cagcgcgaca
     3301 ccgcttggcg acttactgta aggcatgtgc gaacggtttg ccgctgcaat cgccgcctga
     3361 gaaagcgcat caccagtcag cgcatagccg tgatcctgct cgtccatcag cagcgtttta
     3421 atctccagat ctttcggccc aaaggcatcc ggcagatagt cacgcagcgc gtgtgcctcg
     3481 cggcccggca gatgaatacg cagatccaga ccgctgttca gttcattcat aaactgacgg
     3541 cagtgaccac aaggcgtgta gttaacggtg atggctgcaa gcgctttttc accactcaac
     3601 caggcgtggc tgatcgcgct ttgttcggca tgaacggttt gctgcattgt cgcaccaata
     3661 aattccatat tggcaccgaa ataccaggtt ccgctcacac cgcgcgcaat tgcgccaaca
     3721 ttaaaattcg acaatggcgt acgcgcacag gccgctgcca gcggaagtag tgcgaatgcc
     3781 agcgcgtctt cgtccagccc cgttgcgctc ttcagcgatg agacttgctc cccggtcaac
     3841 aaagcgggga agtacttgtc tgccagaata ggttccagtg cagattgcaa gttatccgca
     3901 agttgggcaa aagcggtttg aaaacgtgga tgcatggcgt tgcctcataa tcatgtaatg
     3961 ggataacagt ttacgaaccc gttatggctt tatatgtgat ctgaatctca ttaattatgc
     4021 aacgcgtgaa acttacatca aaaatgcgcg acgcatcgca aattttagcc cattactgcc
     4081 agaataatcg ggaaaaggaa cggtgcgatc agcgaagtaa ttatcccgca taacaccagc
     4141 gctagcgaac taaatgcacc ttcctgataa tccagctcgg cgcagcgcgc cgtaccgagg
     4201 gcgtgcgagg cagtccccat tgccagaccg cgcgcagctt tggtacgaat atgcatcgca
     4261 ttaagcaatg tatggccaaa taccgcgccg aggatgccga cgaaaatcac gcaaactgcg
     4321 ctgattgccg gaataccgcc aatactgccg ccaaccgcca ttgcaatagg cgtggtgact
     4381 gatttcggca ggattgacgc ggcgatttcc ggtgaagcgc ccatcaacaa tgccacggaa
     4441 gtccccgtca ccatcgcaac cacgctgccg ataaaacaga tggtgatgat cgatttccag
     4501 cgcgcgcgga tctggtgtag ctgctcatat aaaggatagg ccagcgcgac gaccgccggt
     4561 tgcagcagat cgtttagcac ttcgctacct ttaaagtagc tgtcgtaaga gatgccggtc
     4621 agcattaaaa aagggatgat caccaccatt gctaccagca acgggttaag caagggaaac
     4681 ttataccgtg ccgccagttt gcgggcggca aaaaagacga tcaatgtcag tggtaatgac
     4741 caccagatat tagccatcat tcttctgatc ctttctgacc tacgacttta cgttcaccgt
     4801 gcaccagttg cgaactccag ctcaccacca gaaaaaccac cagtgtactg acagcacagg
     4861 aaaccactac cgggccaaac tgtgcgcgga gcaaatcaaa atattgcatg acgcctacgc
     4921 caatcggcac aaacaatagc gccatatagc gaatcagtac gtagcacccc ggattgaccc
     4981 attttgccgg aagaatttgc aaggccagca ggacaaacag gatcagcatc ccgatgatgc
     5041 tgcccggaat ggttaccggt agcagggaag caatgaaaat gcctgcatac aggcaggcat
     5101 aaatcaggac gaaagcgcgt aaatattgcc agataatgtt cagtgtctta ctcataatca
     5161 acaccccaga tgagatgaat tcatcataca attaattcgt aaaacgtgct accgatcaca
     5221 ttaagaaatc agcgcttatt ttttcgctcc ccggcattac gcaatgcgca acgaactcag
     5281 gcatacatca agcatagaga gggattacag tttgcagcaa tccctctttg cagaccttta
     5341 atccgttgac cataggcgct aacatagcgc ctcatttttt tgcgggtgaa gatatgcgtg
     5401 tgttactggc tccgatggag ggtgtgcttg actctctggt gcgtgaattg ctgaccgaag
     5461 ttaacgacta cgatctgtgc atcaccgagt ttgtccgcgt ggtggatcaa ctgctgccgg
     5521 taaaagtttt tcatcgcatt tgccctgagc tacaaaacgc cagccggaca ccatccggta
     5581 cgctggtgcg cgtgcagttg ttgggtcagt tcccacaatg gctggcagag aatgccgccc
     5641 gtgcggtgga attaggttcc tggggcgtgg atctcaattg cggctgcccg tcgaaaacgg
     5701 tcaacggtag cggtggtggg gcgacgttac tcaaagatcc tgaactcatc tactagggtg
     5761 caaaagcgat gcgtgaagct gtaccggcgc atttgcccgt cagcgtgaaa gtgcgtctgg
     5821 gctgggacag cggtgagaag aaatttgaaa tcgccgatgc ggttcaacag gctggtgcta
     5881 cggagctggt ggtgcatgga cggacaaaag agcagggtta ccgcgcggag catattgact
     5941 ggcaggcgat tggcgagatt cgccagcggc tgaatattcc ggtgattgcc aacggtgaaa
     6001 tctgggactg gcagagtgcg caacaatgta tggcgatcag cggctgcgac gcagtgatga
     6061 ttggtcgcgg ggcgctcaat attcccaacc tgagccgggt ggtaaaatat aacgaaccgc
     6121 gaatgccgtg gccggaggtg gttgctttgc tgcaaaaata tacccgtctg gaaaagcagg
     6181 gcgataccgg gttatatcac gttgcgcgga ttaaacagtg gttgagttat ttgcgtaaag
     6241 aatacgatga agcaacggaa ttatttcagc atgttcgggt gttgaataat tcccctgata
     6301 ttgcaagggc tattcaggca attgatatcg ataagctgcg ataatagtgg aatcgcagtt
     6361 tatcagagac aatgaatcgc ttattcagct tcatcatctt gttgcgctcc tttcatgagc
     6421 taagccacat attgccactg gcgcaaggag cgcgcagggg gaggacaatc gcctcctccc
     6481 cctgcccccc cgggctctgg cgaacaaaat cgccgctgcg cggtaccttc agcttatccc
     6541 cttcggctac cgggtcgggc gcgaggtaac gtccttgtaa gacgcgccct cagcccacat
     6601 ccatgtgggc tgccccggcc ttcggggaac gcttcagcga ttttgacgcc accaacaccg
     6661 gagctgtttg caaatcaagt acacaaaata tctagttcct gacctgacaa cttatatgcc
     6721 atatctgatt tttaaaatat catcttaaat acgccagtca ccaccagtag cccaatcagg
     6781 aaaataatca atacagccca gagtataatt ttcattggcg ttcctttatt tgtgagtgtt
     6841 gaataagtat agga
//
LOCUS       DAJVUJ010000190         6817 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.190, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000190 DAJVUJ010000000
VERSION     DAJVUJ010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6817
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.190"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            332..424
                     /locus_tag="OQQ48_003741"
                     /pseudo
     CDS             332..424
                     /locus_tag="OQQ48_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004120448.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            428..607
                     /locus_tag="OQQ48_003742"
                     /pseudo
     CDS             428..607
                     /locus_tag="OQQ48_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            941..3220
                     /gene="ydeP"
                     /locus_tag="OQQ48_003743"
     CDS             941..3220
                     /gene="ydeP"
                     /locus_tag="OQQ48_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HCS1954349.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHTASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIEHISGLSQTQIAELADAYAAADRTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            3468..3665
                     /gene="safA"
                     /locus_tag="OQQ48_003744"
     CDS             3468..3665
                     /gene="safA"
                     /locus_tag="OQQ48_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HCS1954350.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGRIARDLTHFIINSLQD"
     gene            3740..4501
                     /gene="ydeO"
                     /locus_tag="OQQ48_003745"
     CDS             3740..4501
                     /gene="ydeO"
                     /locus_tag="OQQ48_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HCS1954351.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFTENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQSHTGMNTGNTMSALAI"
     gene            4903..6585
                     /locus_tag="OQQ48_003746"
     CDS             4903..6585
                     /locus_tag="OQQ48_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HCS1954352.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYNLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            6637..>6817
                     /locus_tag="OQQ48_003747"
     CDS             6637..>6817
                     /locus_tag="OQQ48_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCS1954353.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQG"
BASE COUNT         1964 a         1481 c         1506 g         1866 t
ORIGIN      
        1 gtggtgacgg cagttgaaac caatgtgttg aggttgcatg gacggttaag ccgagaatac
       61 ggtagtaagt gagatatggg gtttggtcgt tgttaatgag caaccatgac cgccaacgac
      121 tggattctcc cttggtttat gttggtgcgc ataatgtata ttatgttaaa tcatatctga
      181 gtgatgcgaa gtgcttttct ggtagtcggt attcgttcag ggttccagaa cttaagcctc
      241 tcctctttca taattggcct gtgcatgacg gatataggct ggctggtatg cagatttgcc
      301 gtttgccgca tcttcccagc gttgaagact caagctggca aaccgcgtgc aaatgagcac
      361 gccctctttc catcaacact tcaggcagct tgcgggaatg agtccactac ggtatcagaa
      421 atgggaagtg ggtgtctctc tgatgcgtaa tggaaaaata ctggctacgg gagaaaatgt
      481 ctccctgggg acagtaaata aaagcaaagt atctctgggt ttaagcgcaa cctatggaca
      541 aacaggaaac aaggttgcag ccggaaccgt ccagtcagtc attggtgtca cttttatcta
      601 tgaataattg tgattttgat cttagtctat gcgctccatt cacgaaaatt gcgcggataa
      661 ttattttgtg aaggctatta tcctacacct gtaagaaaat ccgcgcacaa ttgaagccag
      721 agtcttattt tttatattga aaaatatgtt aatttaaaca ctatgagaga tgatgcttaa
      781 cttatcgtat ttaatctatt gtttaacgat aatagattaa aaatagaaag agagtagtcc
      841 cctactctta tatatccatg ttggcgataa tccctttgta tgtactgtgc atcatcgcta
      901 ttacaaatcc taataattca tttccacaca ggataagtag atgaagaaaa aaattgaatc
      961 ctaccagggt gctgcaggtg gttggggtgc tgttaaatcc gtagcgaatg cagtacgtaa
     1021 gcagatggat atacgccagg atgttattgc catgtttgac atgaataagc cagagggctt
     1081 tgactgtccg ggttgtgcat ggccagatcc taagcacact gcgtcattcg acatttgtga
     1141 aaacggcgca aaagcaatcg cctgggaagt cacggataag caggtaaatg cctctttctt
     1201 tgctgagaat acggttcaat cattacttac ctggggagat cacgagcttg aggctgcggg
     1261 acgtctcact cagcctttga aatatgatgc cgtcagcgac tgttacaagc cattaagctg
     1321 gcaacaagct ttcgacgaaa ttggcgcacg ccttcaaagc tatagtgatc ccaatcaggt
     1381 tgaattctat acttcgggcc gcacttccaa tgaagctgcc tttctttatc agctttttgc
     1441 ccgtgaatac gggagcaata actttcccga ctgctccaac atgtgccatg aaccgaccag
     1501 tgtgggtttg gcagcgagta tcggcgtggg taaagggact gtgttgctgg aagactttga
     1561 gaagtgcgat ttagtcattt gcattgggca taaccctggt acaaaccacc cccgcatgct
     1621 gacttcgttg cgcgctttag tgaaacgggg agcgaaaatg atcgccatca atcctttaca
     1681 ggaacgtggc ctggagcgat ttaccgcacc gcaaaacccg tttgaaatgc tgacgaactc
     1741 tgagactcag ttggccagtg cgtactacaa cgtgcgcatt ggtggcgata tggcgttgct
     1801 caaggggatg atgcgcctgt taattgagcg cgatgatgct gcaagtgccg caggtcggcc
     1861 ctcattgctt gatgacgaat ttattcaaac gcataccgtc ggctttgacg agctacgccg
     1921 tgacgttctc aattccgagt ggaaagatat cgaacatatt tctggactaa gtcagacaca
     1981 aatcgccgaa ctggctgacg catatgccgc tgccgatcgc accattatct gttacggaat
     2041 ggggatcact cagcacgaac atggtaccca gaatgtacag caactggtca atctgctgtt
     2101 gatgaaaggt aacattggca agcctggtgc gggtatctgc ccactacgag gtcactctaa
     2161 tgtacagggc gaccgaaccg tcggtatcac cgagaaaccg tctgcagagt ttctggctcg
     2221 cctgggtgag cgctatggct tcaccccacc tcatgcacct ggacatgctg caattgccag
     2281 catgcaagca atatgcacgg ggcaggctcg agcattgatc tgcatggggg gcaactttgc
     2341 actggcaatg ccagatcggg aagcgagcgc tgtaccgtta acgcaattag atttggcggt
     2401 acacgtagcc actaagctta accgctctca tctgctgacc gcacggcata gctatattct
     2461 gccggttctg gggcgtagcg agattgacat gcaaaaaagc ggtgcgcagg cggtaaccgt
     2521 tgaggattca atgtcgatga ttcatgcctc gcgtggcgtg ttaaaacccg ccggtgtaat
     2581 gctgaaatca gagtgtgcag tggtcgcggg aatcgcgcag gcagcactac cccagagcgt
     2641 ggtagcctgg gagtatctgg tggaagatta tgatcgcatt cgcaatgaca ttgaagctgt
     2701 gttaccagag ttcgccgact ataaccagcg catccgacat cctggaggtt ttcacctgat
     2761 aaatgcagct gctgaaaggc gctggatgac gccgtcaggt aaggctaatt tcattaccag
     2821 caaagggctg ttagaagatc cctcttcagc gtttaacagt aagctggtca tggcgacagt
     2881 acgcagccac gatcagtaca acacgacgat ttatggtatg gatgatcgct atcgaggggt
     2941 attcggtcaa cgagatgtgg tctttatgag tgctaaacaa gctaaaattt gccgtgtaaa
     3001 aaacggcgaa agagttaatc ttattgcgct tacgccagac ggtaagcgca gctcacgccg
     3061 catggataga ttaaaagtgg tcatttaccc tatggctgac cgctcactgg tgacctattt
     3121 tccagaatcg aatcacatgc taactcttga taaccacgat ccattaagtg gcattcctgg
     3181 ttataaaagt attccggttg aattagaacc atcaaattaa ttcgcctcct catttcttct
     3241 gctgtcatcc gcacagcaga agaatttctc attgactatt atcgcgcaat ttgctcacat
     3301 tgatcaaatt aaaccacaca ctataagata taaacctctg cctacagctg taagaaactc
     3361 cgctcagtac tgaagtgcca gtcctatttc ctcttttctc cagcctgtta tattaagcat
     3421 actgattaac aatttttaac gttatccgct aaataaacat atttgaaatg catgcgacca
     3481 cagtgaaaaa caaaatcacg caaagagaca actataaaga aatcatgtct gtaattgtgg
     3541 ttgtcttatt actgaccctt acgttgatag ccattttttc ggcaattgat cagctgggta
     3601 tttcagaaat gggtcgcatt gcaagagatc ttacacattt cattatcaat agtttgcaag
     3661 actgaaaata aacagcaaat tataaatatg aaatgttaaa aaagtatcga taaaaacttt
     3721 attgttttaa ggagataaaa tgtcgctcgt ttgttctgtt atatttattc atcatgcctt
     3781 caacgctaac attttagata aagattacgc cttctctgac ggcgagatcc tgatggtaga
     3841 taacgctgtt cgtacgcatt ttgaacctta tgagcggcat tttaaagaga tcggatttac
     3901 tgaaaatacc attaaaaaat atctacaatg cactaacatc cagacagtga cggtgcctgt
     3961 tcctgcgaag tttttacgtg cttcaaatgt accgactgga ttgcttaatg aaatgattgc
     4021 ttatctcaac tcggaagaac gcaatcatca taatttttca gaacttttgc ttttttcttg
     4081 cctgtctatt tttgccgcat gcaaaggttt cattacacta ttaactaacg gtgtgctatc
     4141 cgtttccgga aaagtgagaa atattgtcaa catgaagctg gcgcacccat ggaagctgaa
     4201 agatatttgt gactgcctgt acatcagtga aagcctgttg aagaaaaaac taaagcaaga
     4261 gcaaacgaca ttctcacaga ttctattaga tgccagaatg cagcacgcaa aaaatttgat
     4321 acgcgtagaa ggttcagtca ataaaattgc cgaacaatgt ggttatgcca gtacttctta
     4381 ttttatttat gcgttccgca aacatttcgg caacagtccg aagagagttt ctaaggagta
     4441 ccgttgtcaa agtcacacgg gtatgaatac gggcaacacg atgagtgctt tagctatttg
     4501 attatttgct aacgagtagt caaccacgca cgctacgtaa gaattatatg gggcagccat
     4561 tccctgcccc gcgttgtttt taggcgatat atttattgaa ataaataagt gacatccatc
     4621 acatatttat gcacttgcat aacctgttgc atgattattt atgttctcaa ttcagcattt
     4681 tgtcagtaaa atgcaataat ttattaaata tcaataaatt agttgtttat cggcgagaaa
     4741 ttacttaata gaacagaaag taatgtcaac gctttatgga ctgttatttc ccttttttta
     4801 gctaaatctg ctatctcttt atgtgactaa cttcacttac atccacttat ttctcttcgt
     4861 aaaattactt cggaattaag tacaataaga agaggaacat ttatgaagtc tgcattaaag
     4921 aaaagtgtcg taagtacctc gatatctttg atactggcat ctggtatggc tgcatttgct
     4981 gctcatgcgg cagatgatgt aaagctgaaa gcaaccaaaa caaacgttgc tttctcagac
     5041 tttacgccga ctgaatacag taccaaagga aagccaaata ttatcgtact aaccatggat
     5101 gatcttggtt atggacaact tccttttgat aagggatctt ttgacccaaa aacaatggaa
     5161 aatcgtgaag ttgtcgatac ctacaaaata gggatagata aagccattga agctgcacaa
     5221 aaatcaacgc cgacgctcct ttcattaatg gatgaaggcg tacgttttac taacggctat
     5281 gtggcacacg gtgtttccgg cccctcccgt gccgcaataa tgaccggtcg agctcccgcc
     5341 cgctttggtg tctattccaa taccgatgct caggatggta ttccgctaac agaaactttc
     5401 ttgcctgaat tattccagaa tcatggttat tacactgcag cagtaggtaa atggcacttg
     5461 tcaaaaatca gtaatgtgcc ggtaccggaa gataaacaaa cgcgtgacta tcatgacaac
     5521 ttcaccacat tttctgcgga agaatggcaa cctcaaaacc gtggctttga ttactttatg
     5581 ggattccacg ctgcaggaac ggcatattac aactcccctt cactgttcaa aaatcgtgaa
     5641 cgtgtccccg caaaaggtta tatcagcgat cagttaaccg atgaggcaat tggcgttgtt
     5701 gatcgtgcca aaacacttga ccagcctttt atgctttacc tggcttataa tgctccgcac
     5761 ctgccaaatg ataatcctgc accggatcaa tatcagaagc aatttaatac cggtagtcaa
     5821 acagcagata actactacgc ttccgtttat tctgttgatc agggtgtaaa acgcattctc
     5881 gaacaactga agaaaaacgg acagtatgat aatactatta ttctctttac ctccgataat
     5941 ggtgcggtta tcgatggtcc tctgccgctg aacggggcgc aaaaaggcta taagagtcag
     6001 acctatcctg gcggtactca caccccaatg tttatgtggt ggaaaggaaa acttcaaccc
     6061 ggtaattatg acaagctgat ttccgcaatg gatttctacc cgacagctct tgatgcagcc
     6121 gatatcagca ttccaaaaga ccttaagctg gatggcgttt ccttgctgcc ctggttgcaa
     6181 gataagaaac aaggcgagcc acataaaaat ctgacctgga taacctctta ttctcactgg
     6241 tttgacgagg aaaatattcc attctgggat aattaccaca aatttgttcg ccatcagtca
     6301 gacgattacc cgcataaccc caacactgag gacttaagcc aattctctta tacggtgaga
     6361 aataacgatt attcgcttgt ctatacagta gaaaacaatc agttaggtct ctacaatctg
     6421 acggatctac agcaaaaaga taaccttgcc gccgccaatc cgcaggtcgt taaagagatg
     6481 caaggcgtgg taagagagtt tatcgacagc agccagccac cgcttagcga ggtaaatcag
     6541 gagaagttta acaatatcaa gaaagcacta agcgaagcga aataactaaa ccttcatgcg
     6601 gcggaatttt ccgccgcctt attgagcgag atagcgatgc acgttacagc caagccctcc
     6661 agttttcaat gtaatctcaa atgtgattac tgtttttacc ttgaaaaaga gtcgcagttt
     6721 actcatgaaa aatggatgga tgacagcact ctgaaagagt tcatcaaaca atatatcgca
     6781 gcgtctggca atcaggtcta ttttacctgg caaggcg
//
LOCUS       DAJVUJ010000191         6785 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.191, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000191 DAJVUJ010000000
VERSION     DAJVUJ010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6785
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.191"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..158
                     /locus_tag="OQQ48_003748"
     CDS             <1..158
                     /locus_tag="OQQ48_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1954354.1"
                     /translation="ILQLCRNDSLMIGRESFSVDRITPDDGGCSYIRLRREGAGNVRA
                     GGYYEGA"
     gene            145..732
                     /locus_tag="OQQ48_003749"
     CDS             145..732
                     /locus_tag="OQQ48_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001214489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1954355.1"
                     /translation="MRGLENAIRNLNSLDTRMVPQASAWAVNRVAAKVVSVATRQVAR
                     NTVAGDNQVKGGPLKLVRQRVRVFKASPAGKMYARIRVNRGNLPAIKLGTTCRRAGDG
                     LKVGKYFFRGAFIQQLANGRWHVLRRLPEACFETGKDSKGRPRKNRLPVEVVKIPLAG
                     PLTQAFEDARDRIIAAEMPKQLGYALKQQLRIHLG"
     gene            729..1130
                     /locus_tag="OQQ48_003750"
     CDS             729..1130
                     /locus_tag="OQQ48_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1954356.1"
                     /translation="MNRHTQIRQAVLASLKGECGGNVVLFDGLPAFIDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFVRAQAPDSELDTWMENTIFPAMKDIPVLSGLID
                     TMIPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            1141..1881
                     /locus_tag="OQQ48_003751"
     CDS             1141..1881
                     /locus_tag="OQQ48_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HCS1954357.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGKGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTLATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTITSKEVMTRTVKISGVGRPYLAEEGAETVG
                     VTGLTVAPASVSVKAGATTTLTFTVKPDGASDKAISVHSSDPQTASVTLSGLVATVKG
                     VKQGSVSIVGMTSDGEFVAVAAVTVSAP"
     gene            1940..2326
                     /locus_tag="OQQ48_003752"
     CDS             1940..2326
                     /locus_tag="OQQ48_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HCS1954358.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRTAKFDAQEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASIMTTWSYDALGAGAER
                     VLSLSGMGTIENAGDDDHEALTPEKS"
     gene            2335..2663
                     /locus_tag="OQQ48_003753"
                     /pseudo
     CDS             2335..2663
                     /locus_tag="OQQ48_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308863.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
     gene            2635..5676
                     /locus_tag="OQQ48_003754"
     CDS             2635..5676
                     /locus_tag="OQQ48_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCS1954359.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAAGDAERSSARMQ
                     RFMERQTQAARQTMHAASSAATAASAHAQTVEKNARAHERMAREVEQTRLRVDALNQK
                     MREEQAQARALAEAQDKAAAAFYRQIDSVKQAGAGLQELQRIQQQIRQARNSGGVGQQ
                     DYLALISEITAKTRALTQAEEQATRQKAAFIRQLKEQATRQNLSSSELLRARAAQLGV
                     SSAAEVYIRKMERAGKATHSLGLKSAAARRELGVLISQMARGNFGALRGSGITLANRA
                     GWTGALMSPKGMMTGGVIGGLVAAVLGLGKAWHDGRKEGEEFNRQLALTGHYAGVTVG
                     QLWKLSRAISGNGITQHAAAGALAQVVGSGAFHGNDIGMVAKAAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNALHFLTATQLEQIRVLGEQGRSSDAARIAMSALAEETG
                     KRTSDIDNNLNALGSTLQTLSDWWKQFWDAAMNIGREDSLDAQIDALQEKIQRAKKYP
                     WTNASTQVEYDQQRLNDLQEKKRRKDLQDAKAQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINAMQYADQAVRDAAIQRENERYEKAIKKNTRATRNDEATRLLLQYS
                     QQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISDLDGKKLTADEKSVLARKN
                     ELIQALTLLDVKQQELQKQTALNDLRKKTVQLTSQLADKERALREQHNLDIATAGMGD
                     KQRQRYQAQLRIRQEYRQQLQQLENDSRQKGTYGTEDYRRAEEVLKGSLKRQLNENKR
                     YWQELEVAQGDWKNGAMRAFQNFTADADNAVGTAEQMFTVAFSSAGNALATFCTTGKL
                     NFKSFTSSLLSDMARIMAQMAMMQAVKGVGSLFGFTTNADGGVYQSADLSRYSGTVVN
                     RPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADTGGSGMVMFAPQYNIGINND
                     GTNGQIGPAALKVVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            5676..6005
                     /locus_tag="OQQ48_003755"
     CDS             5676..6005
                     /locus_tag="OQQ48_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1954360.1"
                     /translation="METFRWKVRPDMNVVSEPKVVAVKLGDGYEQRRPAGLNNLLSTY
                     SVTIRVRKGEHPPLKAFLERHGGVRAFQWTPPYDWKPIRVACRKWSASVGALWVTVTA
                     DFEQVVN"
     gene            6005..6785
                     /locus_tag="OQQ48_003756"
                     /pseudo
     CDS             6005..6785
                     /locus_tag="OQQ48_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001428152.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
BASE COUNT         1631 a         1558 c         2203 g         1393 t
ORIGIN      
        1 atattttaca gctgtgtcgc aatgattcgc tgatgattgg ccgggagtct ttcagcgtgg
       61 atcgtattac tcctgatgac ggcggatgta gttatatccg gttgcggcgt gaaggagcag
      121 gaaacgtaag ggcaggggga tattatgagg gggcttgaga atgccatccg caatctgaac
      181 agtcttgata cccgtatggt gccgcaggcc agcgcatggg cagtaaaccg ggttgctgca
      241 aaagttgtct cggttgccac ccggcaggtc gccaggaata ccgttgcggg agataaccag
      301 gtgaaggggg gccccctgaa actggtgcgt cagcgcgtcc gggtatttaa agccagtccg
      361 gcaggaaaaa tgtatgcccg tatccgtgtt aaccggggca atctgcccgc cattaagctt
      421 ggcacaacat gccggcgtgc cggtgacgga ctgaaggtgg ggaaatactt ttttcgtggt
      481 gcgtttatcc agcagctggc gaacggtcgc tggcatgttc tcaggcgtct tcctgaagcg
      541 tgttttgaaa caggaaagga cagtaagggg cggcccagaa aaaatcgtct gcccgtggag
      601 gttgtgaaaa ttccgctggc cggaccgctg acacaggcat ttgaggatgc ccgcgatcgc
      661 atcattgccg cggaaatgcc gaaacagctg gggtatgccc tgaaacagca actgaggata
      721 catttgggat gaacaggcac acacaaatcc gccaggcagt actggcaagc ctgaagggcg
      781 aatgcggggg gaatgtcgtg ctttttgacg ggcttccggc atttattgat gcgcaggaac
      841 tgcccgccgt ggcggtgtgg ctgagtgatg cgcagtacac cggaaaaatg acggatgagg
      901 acgactggca ggctgttctg catattgccg ttttcgtccg tgcacaggcg ccggattctg
      961 agctggatac gtggatggag aacaccatat ttccggccat gaaggatatt ccggtgcttt
     1021 ccggactcat cgacaccatg atcccactcg gttttaacta tcaacgtgat aatgagatgg
     1081 ccacctgggc gatggcggaa atcacgtacc agatcacgta cacgaattaa ggaggtggca
     1141 atgaccacac caaatccact ggcaaagacg aaaggtgcgg gaacgacgtt ctggatgtac
     1201 accggcaagg gcgatgcgtt tgcgaaccct ttgtcggaca ctgactggct gcgtcttgcg
     1261 atggtgaagg atctgcaacc tggcgaaatg accgctgatg cagaagatga cacttatctc
     1321 gatgatgaag atgcagactg gaaaacgaca acccaggggc agaaatccgt cggtgatact
     1381 ttggcgacgc tggcctggcg tccgggtgac agcgggcaga aaaaactggt tcagttgttc
     1441 gattccggtg aagtctgcgc gtttcgtatc aaatacccca acggtacggt tgatgttttc
     1501 cgtggctggc tgagttcact gggtaaaacc attacctcaa aagaggtgat gacccgtact
     1561 gtaaaaatca gcggtgtggg gcgtccgtat ctggcagagg agggcgctga aaccgtgggt
     1621 gttaccggac tgacggtggc accggcatct gtcagtgtca aagcgggagc aaccaccaca
     1681 ctgaccttta cagtaaaacc tgacggggcc agtgacaaag cgatcagtgt gcattcgtca
     1741 gatcctcaga ctgcttcggt gaccctgagc gggcttgtgg ccacggtgaa aggcgtgaag
     1801 cagggcagtg tcagcatcgt gggcatgacc tctgacggag agtttgtggc agtggctgcg
     1861 gtgaccgtca gcgcaccata acaggacgat actcatcatt gccccggtta tccggggctt
     1921 tttgcatccg gagaacatga tgtttctgaa acaggatacg tttaattatg aaaaacagtc
     1981 cgtggtgctc agtgagctgt ccgggctgca gagaattgaa tatctgacgt ttgttcagca
     2041 gcgaacggca aagtttgatg cacaggaggg agaactgccg gaggctgaac gacagattgc
     2101 ttttctgcgt atgggaatgg atatcaatgc ctggctggtt tcccgctcac tgtggaatgc
     2161 tgagcagtct caggatgttg agacgctttg cgcatccatt atgacaacat ggtcgtatga
     2221 tgcgctgggc gcgggggcgg agagggttct gtcgctgagc ggtatgggga ccattgagaa
     2281 tgccggggat gatgatcatg aggcgctgac gccggaaaag tcctgacgcg ggaaatgcag
     2341 tttgtcatgc ggcttgcccg ggagttccgg cgggcagact ggcggcggat gctgtcggaa
     2401 atgtcggcca ctgagcttgg tgagggggcg attatttccg gatgcagagc ttcagtgatg
     2461 tgtggatgga tgcgcagttt gcctcgctga aggcattgat cgtgagaatg gtgtccggca
     2521 gcagtgatgc tgcggtggct gatttcagcc ttttaccgga agagaacggg ataccggagc
     2581 gaacggacga agaactgatg catcttgggg aaggtatttc cggaggtgtg cgttatggac
     2641 cagatagcca acctggtcat tgatttgggg attgatgcgg cagagtttaa aaatgaaatt
     2701 ccccgtatca aaaaccttct gaatggtgca gccggtgatg cagaacggtc ttctgcccgt
     2761 atgcagcgtt ttatggagcg tcagactcag gcggcccggc agacaatgca tgcggcttct
     2821 tcggctgcaa cagcagcatc agcccatgcg cagacggtgg agaagaatgc acgggctcat
     2881 gaacgcatgg cccgtgaggt ggaacaaacc cgtctgcgcg tggatgccct gaatcagaaa
     2941 atgcgcgagg aacaggcgca ggccagggca ctggcggagg cgcaggataa agcggctgcc
     3001 gccttttatc gccagattga cagtgtgaaa caggccggtg cggggcttca ggaattacag
     3061 cgtattcagc agcagatccg acaggccaga aacagtggcg gggttggtca gcaggattat
     3121 ctggcgctga tttcggagat cacggcgaaa acccgtgccc tgacgcaggc agaggaacag
     3181 gccacccggc agaaagcggc gtttatccgc cagcttaaag agcaggcaac ccgccagaat
     3241 ctgtcgtctt ctgagttgct tcgcgccagg gcggctcagc tgggggtaag cagtgctgca
     3301 gaagtgtata tccgcaaaat ggagcgggca ggaaaagcca cacattcgct ggggctgaaa
     3361 agtgcggcag cccggcggga gctgggggtg ttaatcagtc agatggcgcg cggcaatttt
     3421 ggtgcgctga ggggatccgg gataacgctg gctaaccgtg ccggatggac aggcgcactg
     3481 atgtcgccga aaggcatgat gactggcggc gttattggcg gacttgtcgc ggcggtcctg
     3541 ggtctgggta aagcctggca tgacggccgg aaggagggcg aggaatttaa ccgtcagctg
     3601 gcgctgacgg gacattatgc cggtgtcact gtcgggcagt tgtggaaact cagccgggcc
     3661 atatccggga atggtatcac gcaacatgcg gcagccggtg cgctggcaca ggtggtgggc
     3721 agtggggcat ttcatggaaa cgatattggt atggtggcga aagctgccgc acagatggag
     3781 cgatcggttg gccagtcggt cagcgatacc ataaatcagt ttaagcggct gaaggatgat
     3841 cctgtaaatg ccgcgaaggc gctggacaat gcgctgcatt ttttgactgc cactcagctt
     3901 gagcagatac gcgtccttgg ggagcagggg cggtccagtg atgcggcccg gatcgccatg
     3961 tctgcgctgg cagaggaaac cggtaaacgc acatctgata ttgataataa tctcaatgcg
     4021 ctgggtagta cgctgcaaac cttgtctgac tggtggaagc agttctggga tgcggccatg
     4081 aatattggtc gtgaagactc gctggatgcg cagattgatg cgttacagga aaaaattcag
     4141 cgcgcgaaaa aatatccgtg gacaaacgcc tccacacagg tggagtacga tcagcagcgt
     4201 cttaacgatc ttcaggagaa aaaacgccgg aaggatttgc aggatgcaaa agcgcaggca
     4261 gaacggaatt accaggagca acagaaacgc cggaatgctg aaaatgccgc gctgaaccgg
     4321 atgaatgaaa cggaagctgc acgacatcag cgggaaattg cgcgtattaa tgccatgcag
     4381 tacgccgacc aggctgtcag ggatgcggcg atacagcgtg aaaatgaacg ttacgaaaaa
     4441 gccattaaga aaaatacacg ggcaacccgt aatgatgagg ccacccggtt attgctgcag
     4501 tacagtcagc agcaggcaca ggtggaaggg cagattgctg ctgccagaca gtcagcaggc
     4561 attgccactg aaaggatgac tgaagcgcat aaacagcttc tggccctgca gcagcgcatc
     4621 agcgacctgg acgggaaaaa gctgacggca gatgaaaaga gtgtgctggc ccgtaagaat
     4681 gagctgattc aggcgctgac gctgctggat gtgaaacagc aggagctgca gaaacagaca
     4741 gcgcttaacg acctgagaaa aaaaacggtt cagctgacca gccagctggc agacaaagaa
     4801 cgtgcactgc gtgagcagca caatctggat attgccactg caggtatggg ggataagcag
     4861 cggcagcgct accaggcaca gttgcgcatc cggcaggaat accggcaaca gttgcaacag
     4921 cttgagaatg acagtcgcca gaaaggcact tacgggacgg aggactaccg gagggctgag
     4981 gaggtgctga aggggagcct gaagcgacaa ctgaatgaaa acaaacgcta ctggcaggaa
     5041 ctggaagtgg cgcagggcga ctggaaaaac ggtgccatgc gggcgtttca gaattttacg
     5101 gcggatgcgg ataatgcggt gggaacggca gaacagatgt ttacagtggc attcagcagt
     5161 gccggtaatg cactggcgac attctgtacc accggtaagc tgaattttaa atccttcacc
     5221 tcttccctgt tgtcagatat ggccagaatt atggcacaga tggccatgat gcaggcggta
     5281 aagggcgtcg gctctttatt cggcttcacg actaatgctg atggcggtgt ttaccagtct
     5341 gctgatttga gtcgctacag tggcacggtg gttaaccgtc cgacgttttt tgcttttgca
     5401 aaaggcgcgg gtgtgatggg ggaagcggga cctgaagcca ttctgccact gcgtcgcggt
     5461 gctgatggta agctgggggt tgtggcggat actggtggtt caggcatggt gatgtttgcc
     5521 ccgcagtaca acatcgggat caataacgac ggcacgaacg ggcagatagg tccggcagca
     5581 ctgaaggtgg tttatgacct cgggaaaaaa gcggccgcgg actttatgca acagcaggcc
     5641 cgtgatggtg gccggttaag tggagcatac cggtaatgga gacgtttcgc tggaaggtgc
     5701 gcccggatat gaatgtggta tcagagccaa aagtggtggc agtgaagctg ggtgatggtt
     5761 atgaacagcg tcgcccggcg ggactgaata atctgctgtc gacttacagc gtgacgatac
     5821 gtgttcgtaa aggtgaacac ccacctttaa aagcctttct ggaacggcac ggtggcgtcc
     5881 gcgcatttca gtggacgcca ccttatgact ggaaaccgat cagggtggct tgtcgtaaat
     5941 ggtcggcaag cgtgggggcg ttatgggtga ctgtaacggc cgattttgaa caggtggtga
     6001 actgatgcag gatatccggc aggaaacact gaatgaatgc acccgtgcgg agcagtcggc
     6061 cagcgtggtg ctctgggaaa tcgacctgac agaggtcggt ggagaacgtt attttttctg
     6121 taatgagcag aacgaaaaag gtgagccggt cacctggcag gggcgacagt atcagccgta
     6181 tcccattcag ggggagtggt tttgaactga atggcaaagg caccagtacg cgccccacgc
     6241 tgacggtttc taacctgtac ggtatggtcg ccgggatggc ggaagatctg cagagtctgg
     6301 tcggcggaac ggtggtccgg cgtaaggttt acgcccgttt tctggatgcg gtgaacttcg
     6361 tcaacggaaa cagtgacgcc gatccggagc aggaggtgat cagccgctgg cgcatcgagc
     6421 agtgcagcga actgagcgcg gtcagtgcct cttttgtgct ggccacacca acggagacgg
     6481 atggcgcggt tttcccgggg cgtatcatgc tggcgaatac ctgtatgtgg acctaccgtt
     6541 ctgatgagtg tggttacacg ggcagggcag tggctgacga gttcgacaaa ccaacgacgg
     6601 atatccggaa ggacaaatgc agcaagtgta tgcgcgggtg tgagttgcgc aacaataccg
     6661 gtaatttcgg cggtttcctt tccatcaata aactttctca gtaaatccat gacacagaca
     6721 gaatcagcga ttctggcgca cgcccggcga tgtgcgccag cggagtcgtg tggcttcgtg
     6781 gtgag
//
LOCUS       DAJVUJ010000192         6741 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.192, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000192 DAJVUJ010000000
VERSION     DAJVUJ010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6741
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.192"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            599..883
                     /locus_tag="OQQ48_003757"
     CDS             599..883
                     /locus_tag="OQQ48_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCS1954361.1"
                     /translation="MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMA
                     CKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDPAIEKLLARKPQWDV"
     gene            871..1356
                     /locus_tag="OQQ48_003758"
     CDS             871..1356
                     /locus_tag="OQQ48_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCS1954362.1"
                     /translation="MGCVTAPEPLSSFHQVAEFVSGEAVLDDWLKQKGLKNQAIGATR
                     TFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNMPDPIPVIILARLAVDVSFRGKG
                     LGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
                     Q"
     gene            1390..2568
                     /gene="ltrA"
                     /locus_tag="OQQ48_003759"
     CDS             1390..2568
                     /gene="ltrA"
                     /locus_tag="OQQ48_003759"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCS1954363.1"
                     /translation="MCVATREPPGCDGQTLKMFDQQRDGNLYKIWNRLCSGTWFPPPV
                     LEKRIPKSNGKERILGIPTVSDRIAQGAIKLFMEEKLDPIFHADSYGYRPGKSAHDAL
                     KQCAIRCWRYSWILEVDISAFFDHVKHDLVLKALEHHGMPKWVILYCRRWMEAPMQSC
                     ENGELITRTRGTPQGGVISPLLANLFLHYAFDLWMEREYRGVPFERYADDIVVHCSRM
                     SDATRLKNRLSERFSEVGLVLNAGKTNIAYIDTFKRRNVATSFTFLGYDFKVRTLKNF
                     KGERYRKCMPGASNAAMRKITETIKKWRIHRSTAESLLDFARRYNTIVRGWIEYYGKF
                     WSRNFNYRLWSAMQSRLLKWMQSKYRLSNRKAQRKLTLVRKEYPKLFVHWYLLRASNE
                     "
     gene            2651..2805
                     /locus_tag="OQQ48_003760"
                     /pseudo
     CDS             2651..2805
                     /locus_tag="OQQ48_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2859..3341)
                     /locus_tag="OQQ48_003761"
                     /pseudo
     CDS             complement(2859..3341)
                     /locus_tag="OQQ48_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016154415.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(3597..4577)
                     /locus_tag="OQQ48_003762"
     CDS             complement(3597..4577)
                     /locus_tag="OQQ48_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HCS1954364.1"
                     /translation="MENRKHRPTIGRTLNTNILNNTEEISAPVHVFTLNTGRKAKFTE
                     IKVDHDKVDTQTFVVEEVNGREQTALTPDSLKDITRTIRLQQFYPCIGIRTGDLIEIL
                     DGSRRRAAALLCKVGLRVLVTDDELTVSEAQHLAKDLQTSLEHNIREIGLRLVRLKEA
                     GMNQKQIAEREGLSAAKVTRALQAASVPKDFVSLFPVQSELTYADYRQLAELSERLRL
                     GDISIDEVVKNISPSIELITADDNLSEDEVKNSIMRLITKEMSSLLDSGVKDKAVVTL
                     LWKFDSKDKFARKRVKGRTFSYEFGRLPLEVQDKLDRMIALVLKDNLNSL"
     gene            complement(4577..5776)
                     /locus_tag="OQQ48_003763"
     CDS             complement(4577..5776)
                     /locus_tag="OQQ48_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCS1954365.1"
                     /translation="MTSFEQLSKVAQRADKMLLALTKQIQEQKQEFQADVFYQVYSKS
                     AVAKLPKLTRASVDGAVGEMEAQGYQFEKRPAGTATKYALTIQNIIDIYAHRGIPKYR
                     DRYSEAYSIFIGSLKGGVSKTVSSVSVAHALRAHPHLLSEDLRILLLDLDPQSSATMF
                     LNYLHAVGLVDTTAPQAMLQNVSREELLEDFIVPSVIPGVYVMPASIDDAFIASNWDT
                     LCEEHLLGQNKHAILRENIIDKLKHDFDFILIDTGPHLDAFLKNAIAAADIMFTPVPP
                     AQVDFHSTLKYLARLPELVQIIEQDGCSCRLQANIGFMSKLANKSDHKYCHSLTKEIF
                     GGDMLDVSMPRLDGFERSGESFDTVISANPVTYVGSGEALKNARMAAEDFAKAVFDRI
                     EFIRANY"
     gene            complement(6098..6436)
                     /locus_tag="OQQ48_003764"
                     /pseudo
     CDS             complement(6098..6436)
                     /locus_tag="OQQ48_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(6503..>6741)
                     /gene="istB"
                     /locus_tag="OQQ48_003765"
     CDS             complement(6503..>6741)
                     /gene="istB"
                     /locus_tag="OQQ48_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
                     /protein_id="HCS1954366.1"
                     /translation="EAKLFFQVIAKRYEKSAMILTSNLPFGQWDQTFAGDAALTSAML
                     GRILHHSHVVQIKGESYRLRQKRKAGVIAEANPE"
BASE COUNT         1818 a         1528 c         1603 g         1792 t
ORIGIN      
        1 gcggcatccg gcatataggc agcatgaacg caacacaggc tctcgtacgg aacgtgggaa
       61 cctgtcgtct cgatgctaag ggagcggcgc ggagcagagg cactgcaagc gcctgagtac
      121 cgatgcgaga cacaggggca gatggcttcg tagtagtgag gaagtgcctg taatgggcat
      181 ggagcgaagg gggtcagtcg aggcgtttga ggttaccaat cgaagctata cccggtaatg
      241 gcgcaggttg acctgcactt ggccaagtgg attgcccgca aacacaagcg agcgcgaggt
      301 agtctggtgc gtgcattcga gtggttactg cgaattcgtc acgactgccc gacgctgttt
      361 gcacactggt gcttagctta cgatacttga atggttcaac aacaagagcc gtatgacgcg
      421 agagtgtcac gtacggttcc gtgggagcgt aggggggcgg tgcccctgcg ccacccgata
      481 tcccctttga gtgcggttac gtccacagag gtaaatgccg ctgacgcggc atctgtaatc
      541 tgttactttg tgtacatcat ttgctatata ttgtcaatat gctattgttg taaagcaaat
      601 gatatacgga ggctttatga aatcaggtgt tcagctcaat ctcagagcaa gagaatcaca
      661 gcggatactc attgatgctg ctgcagaaat tcttcataag tcgcgtacag atttcattct
      721 ggaaatggcc tgtaaagctg ctgaagatgt tattctcgac cggcgggtgt ttaattttaa
      781 tgacaggcaa tatgaagagt tcattgaaat gcttgatgct ccggttgcag atgatcctgc
      841 catcgaaaaa ttgctggcaa ggaaacctca atgggatgtg taactgcacc agaaccttta
      901 tcctcatttc atcaggtcgc tgaatttgtc agcggtgaag ctgtacttga tgactggtta
      961 aagcagaagg ggctaaaaaa tcaggcaatc ggagccaccc ggacttttgt tgtatgcaga
     1021 aaaggtacgc aacaaatagt cggtttttac tcactggcca ctggtagtgt caatcataca
     1081 gaagctacag gtaaccttcg gcgcaatatg ccagacccca ttcctgtaat tatacttgca
     1141 cgcctggctg tagatgtgtc atttcgagga aaagggcttg gcgcggattt acttcatgat
     1201 gcagttcgtc gttgttacag ggttgccgaa aatatcggtg tgcgtgcaat tatggttcat
     1261 gcgcttacgg aaaacgcaaa acagttttat attcatcatg gttttaagcc atcaaaaact
     1321 caggtgcaaa cattgtttct taaactccct caatagggag taatgcactg gtctgggcat
     1381 catatctgga tgtgcgtcgc aacaagggag ccccccggat gcgacgggca gactttgaaa
     1441 atgttcgacc agcaacgtga tggcaatcta tacaagatct ggaaccggct atgttcggga
     1501 acatggtttc cgccaccagt gctggaaaaa cggatcccga agtcgaatgg caaggaacgc
     1561 attctgggga tcccaacagt atccgatcga attgctcagg gagcgataaa acttttcatg
     1621 gaagaaaagc ttgacccgat tttccatgct gattcatatg gttaccgacc aggcaaatca
     1681 gctcatgacg ctctgaaaca atgtgccatc cggtgctggc gttatagctg gatactggaa
     1741 gtggacatca gtgcgttttt cgaccatgtg aaacatgatc tggtgctcaa agctctggaa
     1801 caccacggga tgcctaaatg ggtcatcctg tactgtcgac gctggatgga agcgccaatg
     1861 cagagttgtg aaaatggaga attaataacc cgaacacgag gcactccgca gggcggggtc
     1921 attagtccgc tacttgctaa cttgttcctt cactatgcat ttgatttgtg gatggaaaga
     1981 gaatatcggg gggtaccgtt tgagaggtac gctgacgata ttgtagtaca ttgttcacga
     2041 atgagtgatg caacaaggct aaagaacagg ttatcggagc gcttctcgga agtcgggctg
     2101 gtcctgaatg cagggaaaac gaatatcgcc tacattgaca cgtttaaaag gagaaacgtc
     2161 gcaacgagtt tcacctttct cggatatgac ttcaaagtgc gtacgctgaa gaatttcaaa
     2221 ggcgaacggt accgaaaatg catgccgggt gcgtcaaatg cagcaatgcg caaaataaca
     2281 gaaacaatca agaagtggcg tatacatcgc tcaacagctg agagtttgct ggattttgcg
     2341 aggcgctaca atacgatagt gagaggctgg atcgagtact acggaaagtt ctggtccaga
     2401 aatttcaact atcgactgtg gagtgcaatg cagtcacgtc tgctcaagtg gatgcagtct
     2461 aaatacagac tttcgaaccg gaaggctcag cgaaagctga cgctggtaag gaaggagtat
     2521 ccgaagctat ttgttcactg gtatttacta cgtgcatcaa atgagtgatc aagagccgta
     2581 tgacgcgaga gtgtcacgta cggttccgtg agcagccggg gagtgaaatt ctctccggct
     2641 gactcgactc gcgggttccg acagcgatga cgaacatgcg gcggtgttgt actcgctgat
     2701 cggcacatgc cgtctgaaca atgtggaacc agaaaatggc tgcgttacgt cattgagcat
     2761 atccaggact ggccggcaaa ccgggtacgt gatctgttgc cctagaaagt tgatctgagc
     2821 tctcagtaaa tatcaatacg gttctgacga gccgcttact tttggcgtaa ttctgctgcg
     2881 cctttactga acacaatgcc gtattcagca agaagtccac gaatgtgatt tatcagttgt
     2941 gtcctttcca ttgaaaccaa ccgacgcata ttcaaaaaag tttggacgtc ctgttgctcc
     3001 agagttttta ttggtacggg tcgtatagtt ccatgttggc aggcctcagc tattgcctgt
     3061 gcgtcgttat aatcattttt ctggcaccgc tgataagctt taacatgttg tgccggaagc
     3121 aatattacat catggtcgag tttcctgatt tcccgggccc agtaatgaga tgccccgcca
     3181 gcctccatcg caacagtcat tttaggctgt tgcaccagcc agttaagcag ttgattgcga
     3241 gaaagtttgg tctttcctgc gggtttgcca tgagcattga tactgaaaag ataaaagttc
     3301 gttttagcca gatcaatgcc ggtgattttc tgttcagaca tggtatcccg cttacttttg
     3361 tggttaaagt ttatccatca tggcgcaatt gaaggctgcc gtgaaagagg gggaagacta
     3421 tctcatcggt tactgttaaa cggctaagag aaactatctt tttcaagcaa agaagatacg
     3481 gtgaaacagg cgatttcacc gtgaaatact gttcaaaaaa aacgccaata aaagcaaaat
     3541 ttcttttaaa acagccaatt aaaaactggt gagttgctgg cgaaaaactt gaattttcac
     3601 agcgaattca gattatcttt tagtacaaga gcaatcatac gatctaactt atcctgaact
     3661 tccaacggta atctgccgaa ttcatatgag aacgtacgtc cctttactcg ttttcgagca
     3721 aatttatctt tactatcaaa tttccataac aaagttacaa ccgctttatc ctttactcct
     3781 gaatcaagca atgaagacat ttccttggtt ataagccgca taatactgtt tttaacctcg
     3841 tcttcagaaa gattgtcatc agcagtaata agctcaatac tcggcgagat attcttcaca
     3901 acttcatcaa tagatatatc acccagcctc agtcgttcac ttaattcagc cagttgcctg
     3961 taatcggcgt atgtcagctc agactgaaca ggaaaaagag acacaaaatc tttaggtacg
     4021 gacgctgctt ggagggcacg agtcacctta gctgccgaca atccctctct ttccgcaatt
     4081 tgcttctggt tcataccggc ctctttgaga cgtactaaac gcaacccaat ttcacgaata
     4141 ttatgttcaa gtgacgtctg caaatctttc gccagatgct gagcttcaga aacagtaagc
     4201 tcatcatccg ttacaagtac acgtaatcca accttgcata gcaaagctgc tgcccgacga
     4261 cgggaaccat ccaaaatctc aataagatcg ccagtccgaa tcccaataca tggatagaat
     4321 tgttgtaaac gtatagttct ggtgatgtct tttagtgagt ccggcgtcag ggctgtctgt
     4381 tcgcggccat tcacctcttc aaccacaaaa gtttgagtgt ccactttgtc atgatcaacc
     4441 ttgatctcag taaattttgc ctttcggcca gtattgaggg taaatacatg aactggagcc
     4501 gatatttctt cagtattatt caggatattt gtattcagcg tacggccaat agtcggtcgg
     4561 tgctttctgt tttccatcag tagttggccc ttataaattc aatacgatca aaaactgctt
     4621 tagcgaaatc ttctgctgcc atacgtgcat ttttcaaagc ttcaccactg ccaacataag
     4681 taacaggatt cgcagatatc acggtatcaa acgactcgcc gcttctctca aaaccatcaa
     4741 gacgtggcat tgaaacatca agcatgtcac caccgaaaat ctcttttgtc aggctatggc
     4801 agtatttatg atctgactta ttcgccagtt tcgacatgaa gccaatattt gcctgcaaac
     4861 ggcatgaaca tccatcctgt tcaataatct gtacaagttc aggtaaacga gccaaatatt
     4921 taagcgtgga atggaaatca acttgtgctg gcggcaccgg tgtaaacatg atatctgcgg
     4981 cagcaatggc atttttcaaa aatgcgtcca gatgcgggcc tgtatcaata aggatgaagt
     5041 cgaaatcatg cttcagctta tcaataatat tctcacgcaa tatcgcgtgt ttgttttgtc
     5101 ctaatagatg ctcctcgcac agagtatccc agtttgatgc aataaaggcg tcgtcaatag
     5161 aagccggcat aacataaacg ccagggataa ctgacggaac gataaaatct tccagtaatt
     5221 cctcacgact cacgttctgc aacatagcct gcggtgcggt tgtatcaacc agccccaccg
     5281 catgcaagta gttcagaaac atagtggctg atgactgggg atctaaatca aggagcagaa
     5341 tacgtaaatc ttctgacagc aggtgaggat gagcacgcag ggcgtgagct acggaaacac
     5401 tactcacggt tttagacaca ccgcctttta gactaccgat gaatatggaa tatgcctcac
     5461 tataacgatc gcgatattta ggaatgccac gatgtgcata gatatcgata atgttctgga
     5521 tggttaatgc atattttgtc gcggttcctg ctgggcgttt ttcaaactga tagccctgag
     5581 cctccatctc accgactgca ccatccacgc ttgcgcgcgt taattttgga agcttagcta
     5641 cagcagactt tgaatagact tgataaaaaa catcagcctg aaactcctgc ttttgctcct
     5701 gaatctgttt tgttagggcg agcaacattt tatctgcacg ctgcgcaacc ttacttaact
     5761 gctcaaaact agtcattccc cctccgtgct atgtaggatt ttaacttatt acaaaaaaaa
     5821 ctgcattagc agggtaatgc atgcaagtgc atattgtaca tgcttatatt aaaaatgcgc
     5881 gcattgctga tttattaatc atcggaatga atattactgt aagatagtat gcctttccgg
     5941 gagaaagacg ctgatttccc ccgaataatc cagttcacca ccacactccg gacaacagct
     6001 ttcttcaggc tccagatggt gtatttcgcg ggaaacatgt tccggtaacg gacggcgatg
     6061 acgtgactgt cgcaacaggc gggggtagtg cttccaatca gtagaacatg tgcttttcaa
     6121 tgaaggagcc gatgactttt tcgtggattt ccacggaacg cgagaagcag attgttttac
     6181 gtgccagccg cttgatgcgg gtgcggaggg tccggttgtt acgttcaata cgttgcgtga
     6241 agattttgcc ggtcaggtgt ttctccttcg gcacttctct ggcgtagctg ccccagtcat
     6301 cgctggtcac catgccgata cagaacggag tcaacagggc taacagttcc cgacaggtct
     6361 catcattccg tggcccgaaa gtgtaagcca acacaccgcc agttttagtg ttgtacgcat
     6421 accagagcca gtgttgtgtc aacgacggat gaaaagtgat ccacttatat ctccaccaac
     6481 ggcccaatat tgatccaccg ttttactcag gattagcttc tgctataacc ccggcctttc
     6541 gtttctgtct gagtcgatag ctttctcctt tgatttgaac gacatgtgag tggtgtaaga
     6601 tacggcccag catcgctgag gtcagtgctg catcaccggc gaacgtttga tcccactgcc
     6661 cgaacggtag attggatgtc aggatcattg cgctcttttc ataacgttta gcgatgacct
     6721 ggaagaacag ttttgcttct t
//
LOCUS       DAJVUJ010000193         6721 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.193, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000193 DAJVUJ010000000
VERSION     DAJVUJ010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6721
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.193"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            40..246
                     /locus_tag="OQQ48_003766"
                     /pseudo
     CDS             40..246
                     /locus_tag="OQQ48_003766"
                     /inference="COORDINATES: protein motif:HMM:TIGR02601.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            991..2904
                     /locus_tag="OQQ48_003767"
                     /pseudo
     CDS             991..2904
                     /locus_tag="OQQ48_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001306477.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(3032..3754)
                     /locus_tag="OQQ48_003768"
     CDS             complement(3032..3754)
                     /locus_tag="OQQ48_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HCS1954367.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     LTNTFKLGPGVDVNYMLHTQNK"
     gene            3901..6528
                     /gene="gyrA"
                     /locus_tag="OQQ48_003769"
     CDS             3901..6528
                     /gene="gyrA"
                     /locus_tag="OQQ48_003769"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HCS1954368.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
BASE COUNT         1616 a         1656 c         1938 g         1511 t
ORIGIN      
        1 ggctatggag atggtccttc cactgcggcg ggtggcttta tgtatctcgg cttaagtgaa
       61 gttacctttg atattgccga cggaaaaacg ctggttatcg gcaatacaga gaatgacgga
      121 gctgttgact ctattgctgg taccgggttg atcaccaaaa caggttccgg cgatctggta
      181 cttaatgcag ataacaatga ctttactggc gagatgcaga ttgaaaacgg tgaagttacc
      241 ctgggccgca gcaactccct gatgaatgtc ggcgatacgc attgccagga cgatccgcaa
      301 gactgctacg gtctgacgat agggagtatt gataagtacc agaatcaggc agagctaaac
      361 gttggctcca cccaacaaac ctttgtgcac tcattgacgg gctttcagaa tggcacttta
      421 aatatcgatg ctggtggcaa tgttactgtt aatcaaggta gttttgctgg caccatcgaa
      481 ggtgctggtc agctcaccat tgcgcaaaac ggcagctacg tgctgtcggg ggcgcagtcg
      541 atggcgctaa ccggcgatat agtcgttgat gatggtgcgg tgctttcgct ggaaggcgac
      601 gcggcagatc tcgccgctct ccaggacgat ccgcagtcga tcgtgttaaa cggcggtgtg
      661 ctcgatctct ctgatttctc cacctggcag agcggcacat catacaatga tggccttgaa
      721 gtcagtggca gcagcggaac ggttatcggc agtcaggatg tggtagatct tgcaggtggc
      781 gacaatttgc atatccgcgg cgacgggaaa gatggcgtct acgtggtggt cgatgcgagc
      841 gacgggcagg taagtctggc aaacaataat agttatttgg gcacaacaca aatcgcctcc
      901 ggtacgctga tggtgagcga caactcgcag cttggagata cccactataa ccgccaggtt
      961 atctttaccg ataagcaaca agaaagcgtg atggagatta ctgccaatgt cgatactcgc
     1021 tctacgacga ctgagcatgg gcgtgatatt gaaatgcgcg ccgacggtga agtggcagtt
     1081 gatgcggggg tagacacgca gtggggcgca ctgatggctg acagcagcgg gcagcatcag
     1141 gatgagggta gcacatttac taaaacgggg gcgggtacgc tggagctgac cgccagcggt
     1201 acaacgcagt cggcagtacg tgtcgaagaa ggcaccctga aaggcgatgt tgcggatatc
     1261 tttccttatg cttcgtcact gtgggtcggt gatggggcga cgttcgttac tggcgcggat
     1321 caggatattc agtctatcga tgctacttcc agcggcacta tcgatatcag cgatggtacg
     1381 gttttgcgtc tgaccgggca ggatacttcc gtcgccctta atgcctcatt gtttaacggc
     1441 gatgggacgc tggtgaatgc taccgatggc gtgacgttga caggtgagct taataccaac
     1501 cttgaaactg acagcctgac ctatcttgcc gacgtgacgg ttaatggtga tctgaccaat
     1561 acgtccggtg cggttagcct gcaaaatggc gtcgctggcg atacgctaac ggtaaacggt
     1621 gattatactg gcggcggtac gctactgttc gacagcgaat taaacggcga tgactcggta
     1681 agcgatcaat tggtgatgaa cggtaatact gctggcaaca caactgtggt ggttaactcc
     1741 attacaggga ttggtgagcc gacatcgaca ggcattaaag tggttgattt cgcagctgat
     1801 cccacgcagt ttcaaaacaa tgcacagttc agtctggctg gcagtggcta cgtcaacatg
     1861 ggagcgtatg actacacgct agtggaagat aacaacgact ggtatctgcg atcgcaagaa
     1921 gtaacgccgc catcgccacc tgatccagac ccggacccaa cacccgatcc tgacccaaca
     1981 cccgatccaa tccccgctta ccagccggtg ctgaatgcca aagttggcgg ttatctcaat
     2041 aacctgcggg cggcaaatca ggcgtttatg atggagcgac gcgatcacgc tggtggcgat
     2101 ggtcagacgc tgaatttacg tgttatcggc ggacgttatc attacacggc ggcagggcaa
     2161 ctggctcagc aggaagacac ttctacggtg cagcttagcg gcggcttgtt tagcgggcgc
     2221 tggggcacgg atggcgagtg gatgcttggg gcggttggtg gctacagtga taatcagggc
     2281 gacagccgct cgaatatgac cggaactcgc gccgataacc agaaccacgg ttatgccgtt
     2341 gggctgacct caagctggta tcagcacggt aattagaagc agggggcctg gctggatagc
     2401 tggctgcaat acgcgtggtt taacaatgat gtttccgaac atgaagatgg cgtggatcgt
     2461 taccattcgt cggggattat cgcctcgctg gaagccgggt atcagtggtt accggggcgt
     2521 ggtgtggtga ttgaaccgca ggcgcaggtg atttatcagg acgtgcagca ggatgatttt
     2581 accgccgcta accatgcgcg cgtgtcacaa tcgcaggggg atgatattca gacgcggctg
     2641 ggtttacaca gcgaatggcg taccgcggtt ggtgtcacac caacattaga tctgaattat
     2701 tatcacgatc cccatgcgac ggaaattgaa gaagatggca gcactatcag tgacgatgcg
     2761 gtgaagcgac ggggtgaaat aaaagtggga gtcacgggca atatcagtca gcgagtgtcc
     2821 ctgcgcggca gcgtggcgtg gcagaaaggg agtgatgatt ttgcccagac ggcagggttt
     2881 ttgtcgatga cggtgaaatg gtaatcactg tctagtcggc acggttgccg gatgcggcgt
     2941 aaacgcctta tccggcctac ggggagcatt tgtaggcctg ataagacgcg gcaagcgtcg
     3001 catcaggcat tggctcggcc aaacaacaac ctcacttatt ctgcgtgtgc agcatatagt
     3061 tcacatccac gccggggccg agtttaaaag tattagtgag cgggttgtaa tgtagcccag
     3121 ttatatgtcg ctctttaagg cttgtctgat ctacccagcc aagcagttct gccggtttaa
     3181 taaacttctt cacatcatgc gtgcctttgg gcaccatgcg caaaatatat tccgcgccaa
     3241 ccaccgccat cagccatgac ttgccgttgc ggttaagcgt cgagaaaaag acatcgccgc
     3301 ctggtttcac cagttgcgca caggctctga ccactgactg cggatcgggg acgtgctcca
     3361 gcatctccat gcaggtcacc acatcatact gcccggcatg ttttgccgcg tgctcttcca
     3421 cggtttcctg cacataatcc acctgaatgc cgctttccag cgcgtgcagt tttgccacct
     3481 gcaatggctc aaagcccata tccagaccgg tcaccgtcgc gccttcgcgc gccatactct
     3541 cggccagaat gccgccgcca caaccgacat cgagcacctt tttgccaaat aaaccgccag
     3601 cacgctcggc aatatagccc agacgcagcg ggttaatgcg gtgcaacggt ttgaactcac
     3661 cttcaagatc ccaccagcgg gaggcgacgg cttcaaattt agcgatctct tcgtggtcta
     3721 cgttatggtt ttccggcgat ttttcggcat tcattggcac ttctactccg taattggcaa
     3781 gacaaacgag tatatcaggc attggatgtg aataaagcgt ataggtttac ctcaaactgc
     3841 gcggctgtgt tataatttgc gacctttgaa tccgggatac agtagaggga tagcggttag
     3901 atgagcgacc ttgcgagaga aattacaccg gtcaacattg aggaagagct gaagagctcc
     3961 tatctggatt atgcgatgtc ggtcattgtt ggccgtgcgc tgccagatgt ccgagatggc
     4021 ctgaagccgg tacaccgtcg cgtactttac gccatgaacg tactaggcaa tgactggaac
     4081 aaagcctata aaaaatctgc ccgtgtcgtt ggtgacgtaa tcggtaaata ccatccccat
     4141 ggtgactcgg cggtttatga cacgatcgtc cgtatggcgc agccattctc gctgcgttac
     4201 atgctggtag acggtcaggg taacttcggt tccatcgacg gcgactctgc ggcggcaatg
     4261 cgttatacgg aaatccgtct ggcgaaaatt gcccatgaac tgatggccga tctcgaaaaa
     4321 gagacggtcg atttcgttga taactatgac ggcacggaaa aaattcccga cgtcatgcca
     4381 accaaaattc ctaacctgct ggtgaacggt tcttccggta tcgccgtagg tatggcaacc
     4441 aacatcccgc cgcacaacct gacggaagtc atcaacggtt gtctggcgta tatcgatgat
     4501 gaagacatca gcattgaagg gctgatggaa cacatcccgg ggccggactt cccgacggcg
     4561 gcaatcatta acggtcgtcg cggtattgaa gaagcttacc gtaccggtcg cggcaaggtg
     4621 tatatccgcg ctcgcgcaga agtggaagtt gacgccaaaa ccggtcgtga aaccattatc
     4681 gtccacgaaa ttccgtatca ggtaaacaaa gcgcgcctga tcgagaagat tgcggaactg
     4741 gtaaaagaaa aacgcgtgga aggcatcagc gcgctgcgtg acgagtctga caaagacggt
     4801 atgcgcatcg tgattgaagt gaaacgcgat gcggtcggtg aagttgtgct caacaacctc
     4861 tactcccaga cccagttgca ggtttctttc ggtatcaaca tggtggcatt gcaccatggt
     4921 cagccgaaga tcatgaacct gaaagacatc atcgcggcgt ttgttcgtca ccgccgtgaa
     4981 gtggtgaccc gtcgtactat tttcgaactg cgtaaagctc gcgatcgtgc tcatatcctt
     5041 gaagcattag ccgtggcgct ggcgaacatc gacccgatca tcgaactgat ccgtcatgcg
     5101 ccgacgcctg cagaagcgaa aactgcgctg gttgctaatc cgtggcagct gggcaacgtt
     5161 gccgcgatgc tcgaacgtgc tggcgacgat gctgcgcgtc cggaatggct ggagccagag
     5221 ttcggcgtgc gtgatggtct gtactacctg accgaacagc aagctcaggc gattctggat
     5281 ctgcgtttgc agaaactgac cggccttgag cacgaaaaac tgctcgacga atacaaagag
     5341 ctgctggatc agatcgcgga actgttgcgt attcttggta gcgccgatcg tttgatggaa
     5401 gtgatccgtg aagagctgga gctggttcgt gaacagttcg gtgacaaacg tcgtactgaa
     5461 atcaccgcca acagcgcaga catcaacctg gaagatctga tcactcagga agatgtggta
     5521 gtgacgctct ctcaccaggg ctacgttaag tatcagccgc tttctgaata cgaagcgcag
     5581 cgtcgtggcg ggaaaggtaa atctgccgca cgtattaaag aagaagactt tatcgaccga
     5641 ctgctggtgg cgaacactca cgaccatatt ctgtgcttct ccagccgtgg tcgcgtctat
     5701 tcgatgaaag tttatcagtt gccggaagcc actcgtggcg cgcgcggtcg tccgatcgtc
     5761 aacctgctgc cgctggagca ggacgaacgt atcactgcga tcctgccagt gaccgagttt
     5821 gaagaaggcg tgaaagtctt catggcgacc gctaacggta ccgtgaagaa aactgtcctc
     5881 accgagttca accgtctgcg taccgccggt aaagtggcga tcaaactggt tgacggcgat
     5941 gagctgatcg gcgttgacct gaccagcggc gaagacgaag taatgctgtt ctccgctgaa
     6001 ggtaaagtgg tgcgctttaa agagtcttct gtccgtgcga tgggctgcaa caccaccggt
     6061 gttcgcggta ttcgcttagg tgaaggcgat aaagtcgtct ctctgatcgt gcctcgtggc
     6121 gatggcgcaa tcctcaccgc aacacaaaac ggttacggta aacgtaccgc agtggcggaa
     6181 tacccaacca agtcgcgtgc gacgaaaggg gttatctcca tcaaggttac cgaacgtaac
     6241 ggtttagttg ttggcgcggt acaggtagat gactgcgacc agatcatgat gatcaccgat
     6301 gccggtacgc tggtacgtac tcgcgtttca gaaatcagca tcgtgggccg taacacccag
     6361 ggcgtgatcc tcatccgtac tgcggaagat gaaaacgtag tgggtctgca acgtgttgct
     6421 gaaccggttg acgaggaaga tctcgatacc atcgacggca gtgccgcgga aggggacgat
     6481 gaaatcgctc cggaagtgga cgttgacgac gagccagaag aagaataatt ttacttcttc
     6541 atgccaaaag ggagctatct cccttgtttg aattgaaaag tccaggctgc aaagtctggg
     6601 cttttgtcgt attagggcac ggtaaagttt ggctgtgccc gtaaaaaatg gctggctata
     6661 cacaaggaat gtggcaatga gtggtgaaaa aaaggcgaaa ggctggcggt tctatggtct
     6721 t
//
LOCUS       DAJVUJ010000194         6673 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.194, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000194 DAJVUJ010000000
VERSION     DAJVUJ010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6673
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.194"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(812..1252)
                     /locus_tag="OQQ48_003770"
     CDS             complement(812..1252)
                     /locus_tag="OQQ48_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCS1954369.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
     gene            1470..1859
                     /locus_tag="OQQ48_003771"
     CDS             1470..1859
                     /locus_tag="OQQ48_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1954370.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            1903..2307
                     /locus_tag="OQQ48_003772"
                     /pseudo
     CDS             1903..2307
                     /locus_tag="OQQ48_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(2414..3250)
                     /locus_tag="OQQ48_003773"
     CDS             complement(2414..3250)
                     /locus_tag="OQQ48_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HCS1954371.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(3250..3525)
                     /locus_tag="OQQ48_003774"
                     /pseudo
     CDS             complement(3250..3525)
                     /locus_tag="OQQ48_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase APH(3')"
     gene            complement(3600..4073)
                     /locus_tag="OQQ48_003775"
     CDS             complement(3600..4073)
                     /locus_tag="OQQ48_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA14"
                     /protein_id="HCS1954372.1"
                     /translation="MKVSLIAAKAKNGVIGCGPDIPWSAKGEQLLFKALTYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSGWTSNDDNVVVFQSIEEAMDRLAEFTGHVIVSGGGEI
                     YRETLPMASTLHLSTIDIEPEGDVFFPSIPNTFEVVFEQHFTSNINYCYQIWKKG"
     gene            complement(4094..4621)
                     /locus_tag="OQQ48_003776"
                     /pseudo
     CDS             complement(4094..4621)
                     /locus_tag="OQQ48_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
     gene            complement(4682..5497)
                     /gene="sul2"
                     /locus_tag="OQQ48_003777"
     CDS             complement(4682..5497)
                     /gene="sul2"
                     /locus_tag="OQQ48_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCS1954373.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
BASE COUNT         1552 a         1731 c         1745 g         1645 t
ORIGIN      
        1 gcccctgtgg attttgggtc gcctgtcggc cgggaagcgt tatagctttt tcgcttgccc
       61 gcgtacgtgg taatactccg gtattacctt ttatttcttt attttcatgt aattacagtg
      121 gatttttaat acccgggtat tactcgtgaa aacgaaatat tccttttttt tcttttaatt
      181 catgcagttg cgatgaaaat cgcggctgcg ttaggtgtat gaccattttg aggttgaaaa
      241 ataatcaacc atggcgggtg atttacgcag gttaagggga gatcgtgaca aaaaaagcgt
      301 tcctcactgt atcgttcatg cctgcatgaa cgggttattc ttcctgacta cagaattaca
      361 tcgctccttc agttcataca tcacttttac atcacttata catcactttt acatcactta
      421 tacatcactt ttacatcatt ccttcagctc atacgtcact ttcatcctct gtgccatttg
      481 tacatcaccc ccttcctgaa cgtgctgaca gcgggttttc cggctgcgcc ggcaacgctt
      541 cacggccacg caataaattg cgtccccgtt caccgttttc ggatgggaag aacgccggaa
      601 acaggactta ccccggctga atggtgtgaa tttccgcgct atgcacttgt gcgcatactc
      661 atgcatgccg taaaaacaga gcctgcgcgt ttctggcggg ttttcgggtg gtttgttgcc
      721 tgttttaccg gtttcccgtc agaaacgccc tgagggcctc tcaggcggtg ctgataacag
      781 atgttatggt aaatcagagt cacctgcact gttactctga accggagcga aacagccgcg
      841 accagaatcc ccgctttttt tcctgtgcca gcgcgtcagc cagacggcta atttcggcct
      901 gtagttgttc cctctcctca tttcgcaggc ggttttctgc ttcccgcgcc atcttgtctt
      961 caagcattag ggtaacagtc tgctgcagag cgtccagttt ctgctgaatg acgtctagct
     1021 gagcattttc cgcctggcta acgacagatt tggagtgtcc ctccttgtgc ctttttggtg
     1081 tctcagaagt gcgcagctcg ccataggccc gaatcagttc actggtctcg aactcccggc
     1141 gatcattttt tcctgtgcga tagctgacaa ggcctttagc catgtcacga tataattgcg
     1201 agcgcgactt tcctgttaat ttcagtgtct cttgaactgt gtgccatgcc ataaaagtga
     1261 cagtgtctcc tgtgtgtacc gtattgtaac agcagaacat attactgttt taacacaaaa
     1321 ggtctgaggc tgcccgtgag gcgtgaaaaa tacacaaaaa acctgttgac ggtgtggttt
     1381 tgacgggcgt agtatttcga atactgtata taaacacatt gttataaata cagtttttgc
     1441 tgtttttaaa agtcaaagag gataatttta tgagtacaga gttaaatagc ggtactgtcg
     1501 gtggttttgg tcacatggga ccaaatgacc cctacaatga tggtcatagc ggctctggaa
     1561 gttcgggagg tagttttaac ggtggtggta atggtggcca tgataatagt agtggttcat
     1621 cttatactgc atcctttggt ctcatcactg gccgacaccc gataactgtc ccaggtacga
     1681 agaatattaa ttctgtcgat ctttctgctg tcattaataa cattatacat catgaaggtg
     1741 cacgcaataa catgtaccta gatactgcga acaatgtaag agtgggagtg acgggcactg
     1801 gctggcaatg tctagcaacg gcaggcattt cggctgaggg taaaagaact ttccgctaag
     1861 cgatagactg tatgtaaaca cagtattgca aggacgcgga acatgcctca tgtggcggcc
     1921 aggacggcca gccgggatcg ggatactggt cgttaccaga gccaccgacc cgagcaaacc
     1981 cttctctatc agatcgttga cgagtattac ccggcattcg ctgcgcttat ggcagagcag
     2041 ggaaaggaat tgccgggcta tgtgcaacgg gaatttgaag aatttctcca atgcgggcgg
     2101 ctggagcatg gctttctacg ggttcgctgc gagtcttgcc acgccgagca cctggtcgct
     2161 ttcagctgta agcgtcgcgg tttctgcccg agctgtgggg cgcggcggat ggccgaaagt
     2221 gccgccttgc tggttgatga agtactgcct gaacaaccca tgcgtcagtg ggtgttgagc
     2281 ttcccgtttc agctgcgttt cctgtttggg gtcgtttgcg ggaaggggcg gaatcctacg
     2341 ctaaggcttt ggccagcgat attctccggt gagattgatg tgttcccagg ggataggaga
     2401 agtcgcttga tatctagtat gacgtctgtc gcacctgctt gatcgcggcc gcgatagcta
     2461 gatcgcgttg ctcctcttct ccatccgcgt tccaagctgc ggaaaggcac ccataagcgt
     2521 acgcctggtc gagcaggcga cgcggatcga cgtccagcgc acgagagaat gcgtccgcca
     2581 tctgtgcaat gcgtctagga tcgagacaaa ggtcgtctct gtcagccgga tcgtagaaca
     2641 tattggcggc gccaaagccc acttcaccga ccagaccgac gggatctatc accagccagc
     2701 cgcgactgga gaacatgatg ttttcatgat gcagatcgcc atgtagccca cgcagttccg
     2761 aggcattgct catcatttga tcggctataa tcgccgcgtg gacgtagtca gtttgacaac
     2821 ctgcgttttg atcatcgcgc gcccgctgaa acaaagctgc aaagcgatcc cggatcggga
     2881 gaagggcaga aggcaggggt tcctcagatg cggcatacag cttcgccatt agttccgctg
     2941 caatttcggt cgcctggtag tcgccgtgct cggcaacgat gtgagagagc attcgctccc
     3001 cggcatattc gagcaacatc agattgttct cacgaccgag caaccggact gctcccctcc
     3061 cattgcgcca taccagatag tcggccccgc gcagttcatc agcaatgtct tctataggtt
     3121 tcaatccctt gacgattgca ggagtcccgt ctggcaatga aactttccaa acgaggctgg
     3181 aaaaggtgtc cgcaatgaga acaggttgcg aaacgtgcca atgagcagga aaaacaggcg
     3241 gcatgaacat caaccccaag tcagagggtc caatcgcaga tagaaggcaa ggcgttcgcg
     3301 gtcgggggct tcgatcccca atacattgaa taggacagcg aaggcgcgct ctgcttcatc
     3361 tggcgctgcc cagttctctt cggcgttagc aatcatgagt gccaaatcgg catagcgatc
     3421 tgctgttccg agccgcccaa ggtcgatcag acccgtgcat tgaagagttt tagggtccac
     3481 catgaagttc ggcatgcagg gatcaccatg gcaaacaacc atatcgtcgc tatcaatcgc
     3541 ggaaaaaggc gtagtgcgcg aagcgaacga agctttttgc cgtcgattgc atagctttgt
     3601 taaccctttt tccaaatttg atagcaatag ttaatgtttg aagtaaagtg ttgctcaaaa
     3661 acaacttcga aggtatttgg aatactcggg aagaaaacat ccccctctgg ctcgatgtcg
     3721 atcgtcgata agtggagcgt agaggccatg ggtaatgttt ctcggtaaat ttctccgcca
     3781 ccagacacta taacgtgacc ggtgaattca gctagcctgt ccatggcctc ttcgattgac
     3841 tgaaatacaa ctacattgtc atcatttgat gtccaacctg agcgggtaac gaccgcgtat
     3901 ttcctattgg ggagtgcgcc catagattca aacgtcttgc gacccaccag aagccactga
     3961 ttgtaggtca atgctttaaa aagtagctgc tcccctttcg cggaccaggg tatgtctgga
     4021 ccgcaaccaa tcacgccgtt tttcgctttc gcagctatca atgatacttt caaggttctc
     4081 atcctgggtt aacggtgcgc tcttggtcga gccgcaccgg tagctctcgt tcgacacgag
     4141 ccaaaagatc gagctgcggc gtactcttgt cctcgtccgg taagaagtcg ggattgacgg
     4201 cattgcggga caccacatca acggcgcgtc cgaacattcg cgacagcctg cgctcaaacg
     4261 gacattgatc aaccgatagg ctgtgaacag cgccaagttg ctgccccatt gacggccacg
     4321 ctttgagcaa atccgctcca gacagatcag ccgccggtac tcccggaatt gccgttatca
     4381 ccaagcatgc accctcctgt tcctcctgcc agttgatgac ctcggggcaa gccacacctc
     4441 gacctttgag ccaaatgagg cggtcacgct ctccagcgag ctcaccgcgg cgggaagcag
     4501 gtgcgatttt cgcgaaggca tgcccgtcac cacgtcgaaa aacaaaatca ccagattctc
     4561 cgcctctgac aggcaaccag tcagaatgcg attcaccaaa aaaaatatta gttcgattca
     4621 atggaggttc cttcagtttt ctgatgaagc gcgaatatag agaaatatcc cgaatgtgca
     4681 gttaacgaat tcttgcggtt tctttcagcg ccgccaatac cgccagcccg tcgcgcaagg
     4741 ggcgcggctc gtgtgtgcgg atgaagtcag ctccacctgc ggcggcggca agctctgcag
     4801 cgagtgtcgc ggccccgaca tcccccggac cacggcctgt gagcgcgcgc agaaaggatt
     4861 tgcgcgaaac agacagaagc accggcaaat cgaagcgcag ccgcaattca tcgaaccgcg
     4921 ccagcaccga gagcgaggtt tcgggagcag cccccagaaa aaaccccatg ccgggatcaa
     4981 ggacaaggcg gttgcgtttg ataccggcac ccgtcagcgc cgcgatgcgc gcgtcaaaga
     5041 acgccgcaat gtgatccatg atgtcgccag cgggtgcctc gcgccgatct gcctgcccgt
     5101 cttgcaccga atgcataacg acgagtttgg cagatgattt cgccaattgc ggatagaacg
     5161 cagcgtctgg aaaaccgcga atatcattga gataggccac accacgcgac aaggcatagg
     5221 cttgcgtcgc gggttgataa ctgtcgagcg agacgggaat gccatctgcc ttgagcgcgt
     5281 ccagcaccgg cgcgatacgc gcgatttctg tgtcggacga aacaggcgcg gcgtcgggat
     5341 tgctggatgc cggaccgagg tcgatcacat ctgccccctc ggccatcagc ttacgcgcct
     5401 gcgcaatggc tgcgtctggc gccagatacc ggcctccatc ggagaaactg tccgaggtta
     5461 tgttgacgat gccgaaaatg atgagcgatt tattcatggg ggcttctata ataataataa
     5521 tcgagcatga gtctcatacg gatgctcggg tcgaaaggga atccccaggc gagtaacctg
     5581 tttgcggtga tccgtttcat gatgatgtat ctgtagcatt ttggaaataa atatatcatc
     5641 tctcaataag tagatatatt tatctttttt gcttctttgg tgctgcaagt caccaatacg
     5701 agggaaacct gttacggagc ggtttaaccg taatcaacag tgactggtgt catgttcgga
     5761 agattaattg atagtaatct acaaaggatc ttcttgagat ccttttcaat gaatcgtaat
     5821 ctcttgctct gtaaacgaaa aaaccgcctg gggaggcggt ttgttcgaag gttaagtcag
     5881 ttggggaact gcttaacccg gtaacagggt tcacgagacc gccattacca aaactgttct
     5941 ttcagtgtag ccgcagtcag gcctctactt caagacctct taatctatct tctcgtgcac
     6001 tcctgttact aatggctgct gccagtggcg ttttgtcgtg tctttccggg ttggactcaa
     6061 gttgatagtt accggacaag gcgcagcggt cggactgaac ggggggttcg tgcatacagt
     6121 ccagcttgga gcgaactgcc ttcacggaac tgagtgtcag gcgtggaatg agaaaccgcg
     6181 gccataacag cggaatgaca ccggtaaacc gaaaggcagg aacaggagag cgcacgaggg
     6241 agctaccagg gggaaacgcc tgggatcttt atagtcctgt cgggtttcgc caccactgat
     6301 ttgagcgtca gattttgcga tgcttgtcag gggggcggag cctatggaaa aacggcttcg
     6361 ccgtggcctt atctcttctt tgctaagtgt cttcttggta tcttccagga aatttccgtc
     6421 cccgtccgta agccggtacc gctcgccgca gccgaacgac cgagcgtagc gagtcagtga
     6481 gcgaggaagc ggaatatctc ctgtatcgca tattctgctg acgcgccggt gctgcctttt
     6541 ttctcctgcc acatgaagca cttcactgat tttcgcaatc gtggcaacat agtaagccag
     6601 tatacactcc gctagcgcta catgactggt tcagggctgc gccccgaaac ccgctaacaa
     6661 cctctcagct act
//
LOCUS       DAJVUJ010000195         6524 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.195, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000195 DAJVUJ010000000
VERSION     DAJVUJ010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6524
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.195"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(692..2401)
                     /locus_tag="OQQ48_003778"
     CDS             complement(692..2401)
                     /locus_tag="OQQ48_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HCS1954374.1"
                     /translation="MPLNKTFSSSIFSTKNSLSTDMSVNRDNRTITSSIMRVSNSSEL
                     IQFKNKTAPYFSEKRNVKVNINGVAKDIYGRQIVCRHLASYWEMNFMETNGKVNYQLL
                     STPDAIAKNVCLEKTEDFSKSPAYIYFVENKKWGTVITNFFYNMKKNGDFVRTLSACT
                     LNHQMALGLKIKRVQESEKWVVQFFDPNRTVTHKRTVFTCDSHFELSQLSAKDFFDDF
                     YWKIYGLEQPGQVIFEDRHNSPLTNTVKLLPDELINSRVIYHAITKNLTEVLFILMEK
                     YKNGEISQSKLVNLLATRSSDGTPAFYIALQNGCSDIIQVYGKILNMCNLSQETILTL
                     LAAVGANNVPGLCMSFMNGHVDTIKAYGEIVFKTPLTSDKRLYLLAAKDSHDLPGLFF
                     ALQNGHADSIRMFGSLLNKKMLSSEQIKELLKVKHGLFMALQNGHTKAIMAYGDILKI
                     LPPHQEYIDELLWIKNPNGTSGLFMAFYNGHTETIRAFCNILKNYSFTTRRLVEMLSA
                     TNKDGIPGVFVSVVNRDKETILEYCRIIKENNLEPDTIAEQFSKKMKKTFIEIINRFN
                     HFL"
     gene            complement(2833..3552)
                     /locus_tag="OQQ48_003779"
     CDS             complement(2833..3552)
                     /locus_tag="OQQ48_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     phosphothreonine lyase"
                     /protein_id="HCS1954375.1"
                     /translation="MPIKKPCLKLNLDSLNVVRSEIPQMLSANERLKNNFNILYNQIR
                     QYPAYYFKVASNVPTYSDICQFFSVMYQGFQIVNHSGDVFIHACRENPQSKGDFVGDK
                     FHISIAREQVPLAFQILSGLLFSEDSPIDKWKITDMNRVSQQSRVGIGAQFTLYVKSD
                     QECSQYSALLLHKIRQFIMCLESNLLRSKIAPGEYPASDVRPEDWKYVSYRNELRSDR
                     DGSERQEQMLREEPFYRLMIE"
     gene            complement(3677..4240)
                     /locus_tag="OQQ48_003780"
                     /pseudo
     CDS             complement(3677..4240)
                     /locus_tag="OQQ48_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4224..4482
                     /locus_tag="OQQ48_003781"
                     /pseudo
     CDS             4224..4482
                     /locus_tag="OQQ48_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(4591..5169)
                     /locus_tag="OQQ48_003782"
     CDS             complement(4591..5169)
                     /locus_tag="OQQ48_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1954376.1"
                     /translation="MKVSFKSLGYIFHDIYNKKHTIDEFNDVVRKAVLSGKINELNAC
                     HKVAIFLAEKDNEITKKDKAKIIDTLTENYSIEFQQLMNISERTLNSSLYITPGESGF
                     VSFVNREGKICHTAYVKSSDNSMAYYHANYSSIDKYITDMCGLICMRHIESTCIIFYM
                     LDEKVLSAIAEFMNEKGWRAAFCSAKNLYKCV"
     gene            complement(5590..5827)
                     /locus_tag="OQQ48_003783"
                     /pseudo
     CDS             complement(5590..5827)
                     /locus_tag="OQQ48_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(6014..6443)
                     /locus_tag="OQQ48_003784"
                     /pseudo
     CDS             complement(6014..6443)
                     /locus_tag="OQQ48_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1790 a         1489 c         1187 g         2058 t
ORIGIN      
        1 attattggct ccttctgatg ccattctatt tcaggaagga gtgtccgtta aactcaggct
       61 acctcagata agcttgatac aactggtctg gtatatacag tttctttgag tgcggttacg
      121 tccacagagg taacggtaaa agtaacccct tgtcgggacc actccgaccg cgcacagagg
      181 cattttcatc gctgacaggc cgcattgcct cgcagggctg gaggaggatc ggtgatatcc
      241 ggacgaccgt accggcttac gccgggcttc ggcggtttta ctcctctgcc atgtgcaaca
      301 acggagtgcc gccttacatg ccgctggcgc ggcatatcct ggtaacattt ttgttgatac
      361 caccttccgg ccttatcctg accagtgggc tttcctggca tcgatgatac cgatgaacgc
      421 gcatgatatt gagccgacga tattacgggc aactggaaat actcaccctc tggatgtgac
      481 ctttatccat gaagaagatt tggccatacc atggaaacct gaacaatcat cagtttacgc
      541 ccatgtaaac ccctacggga tatttgaact cgatatggaa acaaggttgc caatcgaggt
      601 ggttgcctga aagttggcag ttacccggtc actacacctt gttggagtga acatatcaaa
      661 aacaggtagt tacacaattt aaattacagt ctcataaaaa atgattaaat ctatttatta
      721 tttcaataaa cgtttttttc atcttctttg aaaattgttc tgctatagta tccggttcca
      781 gattgttctc ttttattatt ctacaatact ctaatattgt ttctttgtct ctatttacta
      841 cagatacaaa tacaccagga ataccatctt tattagttgc ggacagcatt tcgaccaatc
      901 gtcttgtggt aaaactatag ttttttagaa tattacaaaa ggcacgtatt gtttcggtat
      961 ggccattata aaatgccata aaaagtcctg atgtaccgtt gggatttttt atccataata
     1021 attcatctat atattcctgg tgagggggga gtatttttag tatatctcca tacgccataa
     1081 tggcctttgt gtgcccattt tgaagtgcca taaacaaccc atgctttact ttcaataatt
     1141 ctttaatttg ttcactacta agcattttct tattaagcag acttccgaac atacgaatac
     1201 tatctgcatg tccattctgc aatgcaaaaa ataacccagg taaatcatgg ctgtctttcg
     1261 cagctaatag atataatctc ttatcggaag ttaacggggt tttaaagact atttccccat
     1321 aggcttttat tgtgtctaca tgtccgttca taaaagacat gcataaaccc ggcacattgt
     1381 ttgcccctac cgcagcaagc aatgtaagta ttgtttcttg actaagattg cacatattta
     1441 atatcttacc atatacttga ataatgtctg aacatccgtt ttgaagtgct atataaaatg
     1501 caggagtacc atcagagctt ctcgtggcca gaaggttcac cagtttagat tgagaaattt
     1561 caccattttt gtatttttcc atcagtataa ataatacttc tgtcagattc tttgttatcg
     1621 catgatatat aactctggaa ttgatcaatt catctggtaa aagtttcact gtgtttgtta
     1681 gaggggaatt atgcctatct tcaaatatta cctgcccggg ttgctccaga ccataaattt
     1741 tccaataaaa atcatcaaaa aaatccttag cggataattg actcagttcg aaatggctat
     1801 cacaagtaaa tacagtacgt ttatgagtaa cagttcgatt aggatcgaaa aactgaacaa
     1861 cccatttttc actttcttgt acgcgcttta ttttcaaccc taacgccatt tggtggttta
     1921 atgtgcatgc tgataatgtt ctgacaaagt ctccattttt tttcatgtta taaaaaaaat
     1981 tagtaataac tgttccccac tttttatttt ccacaaaata aatataagct ggtgattttg
     2041 aaaaatcttc cgttttttcc aaacatacat tttttgcaat agcatcaggg gtgctaagaa
     2101 gctgatagtt taccttccca tttgtttcca taaaattcat ttcccaatag gaagctaggt
     2161 gacggcaaac tatttgtctt ccatatatat cttttgccac tccatttata tttaccttca
     2221 catttctctt ttctgaaaaa taaggtgctg ttttattctt aaactgaatt agttcactgg
     2281 agtttgagac cctcataata gaacttgtta ttgttctatt atctctgtta acagacatat
     2341 ctgttgataa cgaatttttt gttgaaaata tactagatga aaatgtttta tttaacggca
     2401 tagaagacaa ccatagagat gttataatac tattagattg cagttactaa tagtaaaagc
     2461 ctccttacaa ggaggtagct tattcagaat gctcctataa gatagcgagc tcgtcaacga
     2521 tacagatgat ctgattatat tgtgcaaaag gagaacagga aaataggcac tggaaaagat
     2581 gaaggcctga tgggagcatt aactaatgca aaatccgaca ggttaatatc ccgaaaagca
     2641 atcaaacgac tcaaacagga gatgcacgct gaagtggtgg acaactgttc ggatttcaga
     2701 tgagtagctc agcagtttat gagccgaatg ctctgtcacg gtcaggaatc aggctgcata
     2761 cgctgtgcca aatctaatcg tagttattct aaccagtttt ccagctacaa ggtggtgtag
     2821 ctggcatctt ctctactcta tcatcaaacg ataaaatggt tcctctcgta acatttgctc
     2881 ttgcctttca cttccatctc gatcgcttcg tagttcatta cgatagctga catatttcca
     2941 gtcttcaggt ctaacatctg acgccggata ctccccggga gcgattttgc ttcttaatag
     3001 attagactcg agacacatta taaattgtcg tattttatga agtaataaag cactatattg
     3061 cgagcattcc tgatctgatt ttacatatag cgtaaactga gctcctatcc ccacacgaga
     3121 ttgctgagaa actcgattca tatcagttat cttccattta tctataggac tgtcctctga
     3181 aaataataaa ccagacaaaa tttggaatgc cagaggaact tgctccctag ctatactaat
     3241 atgaaacttg tccccaacaa aatccccctt actctgagga ttttcacgac atgcatgaat
     3301 aaacacatct ccactatggt tcacaatctg gaatccttgg tacatgacag aaaaaaattg
     3361 acatatatca gagtaagttg gcacgttcga tgctactttg aaatagtacg ctgggtattg
     3421 ccgtatttgg ttgtacaata tattgaagtt atttttcaat ctttcattcg cagaaagcat
     3481 ctgcggtatt tcagacctca caacattcaa agaatctaaa tttagtttta gacagggctt
     3541 ttttatgggc atagaaaacg tcctctataa aatagatata ttatcttata ttgattgttt
     3601 atcatctttt ataaaacgat acattatatg taatgtatgt aagcgtaagg gtcttctccg
     3661 atcatggtgg gaaggcttag aacgccatat tcagtttccc atagtggaac atcactccca
     3721 gcttaaagcg ctcccggttg cggtagccct tggcttttat tctcagcagc ctgatcttgc
     3781 tgttcagtgc ttccgcattt ccgtttgata cgccatggcg catggcgttc aggataccgt
     3841 acaacctttt ccctatcgtt ttagccacat tcttcatcat ggggacgtca ctgttggccg
     3901 ccagagacag ccatctctgc cagtcgttgc gtctttcctc gctccatggt ctgctccaga
     3961 tatcctttgc cagttctttc agcgcccagc acaggctcgt cagcttcatc tgcgcacgca
     4021 gccatatcag cttttcctgc ctggattcgg tcatccattt atcgctgtgc tgccacagga
     4081 agcgagttcc ctttgcctga cgacgacttt caacaggaag atgcggatgt tcattctggc
     4141 gggttttatc caccacctca cccggttgtt tcgccacatg aaagcgatcg aaggctattt
     4201 tctcaaccgc attgggtaag tggatgcgcg ctgctcttag tttgctgagc cagtttagca
     4261 ttgccttcta catgaccgat gcctgagccg gtataccgaa ctttattgtc ctcaaccagt
     4321 catgttataa gcaagaaatg cactcagcgg atagagcgac ataatctgaa cctgcggaca
     4381 catcttaagc ggcttacccg caaaaccata tgcttctcaa agtctgatga tatgcactat
     4441 aagatcatcg gctggtatct gacaatcaac caccaccact aaatctgcgt cacgacctgc
     4501 aactgagtct ggttgaacag gctgaggccc tgctgaagca acgcgaacct ctcgactctt
     4561 gttggatttt accaaatagt ttttctaaaa ttaaacacat ttatataagt tctttgctga
     4621 acaaaacgca gcccgccatc ctttttcatt cataaattca gcaatggcag ataaaacttt
     4681 ttcatcaagc atataaaaaa taatgcaagt agactcaata tgcctcatac aaataaggcc
     4741 gcacatatcc gtaatatatt tgtcaatgct gctataattt gcatggtaat atgccatgct
     4801 attatctgat gattttacat atgcagtatg acatattttt ccttcacgat taacaaaact
     4861 gacaaaacca ctttcaccag gagtaatata taaagatgaa tttaacgtgc gctcactaat
     4921 attcattaat tgttgaaatt caatggaata gttttcagtt aatgtatcta ttatcttggc
     4981 tttgtctttc tttgttatct cattgtcttt ttcggcaaga aaaattgcca ctttatgaca
     5041 tgcattcagc tcgttaattt tacctgataa aactgctttt cttaccacat cgttgaactc
     5101 atcgatagtg tgttttttat tatatatatc atgaaaaata tagcctaaag acttaaacga
     5161 gactttcatt ttatattctc cttaaatatt aacaggatat atatatcaca ttttcctcta
     5221 tattttcaga aaccagctat cattttcccc attactggta cgtcgcttca gatatttccg
     5281 cagcaagcga taaccctgcg cactatgagg atgccattca ttataatgtt tgaacacctt
     5341 cgcgagacta ctttactgct gttaacacgt caggttcgaa cataacactg atttaatcat
     5401 atttcatcgt tttgacgaag ttttctgcca ttccattttg caccgccgtg tgtttggggg
     5461 caaggccgac catctgtgtt tcattcgcca gatataagtg atgagatggc attctccctc
     5521 cttcacggca gaagcatttt gaccgtttag gggatgcgga cgattttcat gcctgctaag
     5581 atactttcgt tactgatgct gtccgttttc caccatttca tcatcactga ttggcgttgt
     5641 gtttttggtc agcactccag ctttacgttt ctcttttaac cggtaactct ctcctttgat
     5701 attcagcgtg tagcagccga tctaggatcg ctgttgccag cacgtgatct ccgaacattt
     5761 ctccccagtc tgcgacccct ttgtttgacg tcagtatgat gctcgctttt tcatatcgac
     5821 agttcatcga taggaattta aatccccaaa agattaaaaa agcaccgaag aatgcgcgtt
     5881 tattcaacac aacctgatca tcaagtaatc ggtatagaca cttaatattg cttacattga
     5941 ttgattcaat gtatctctga tggttttttg ctccgttccc ctcaacatgg acgcaacttc
     6001 ccctctgcga ctctcagccg caccaccgta ttcgagccag cggctcatgc atcataacct
     6061 gctctgccag acggtaaccc ctcttcagcc ccgtaaaccg catctgacct ccgcacagct
     6121 cgcacttcag cgggtcaacc ttcagtcact gctgatacat ccctctcctt gtgatttgcc
     6181 tcgccgtttt tctcactgtc tccgttatgg cgtacaccac atcttccagt aatcgccgct
     6241 tcaccggact caggaccccg tatcaccatc cggaacccct tatccgccac atgccaggag
     6301 aacctttcca taaactcgtc gccactcatc acaaggtttt cttctcgttt tgtccggtgg
     6361 ctgttgtaac gcaggccaat ttcatcctga ccggcataat gttccaggcg gctcatctgc
     6421 accggctgct tctccagcca gttaagccat cggtttccct gctgaaaaat atcggcaaaa
     6481 cggggaagca tcagaaaggc ggaataacgc catccggcca gtga
//
LOCUS       DAJVUJ010000196         6487 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.196, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000196 DAJVUJ010000000
VERSION     DAJVUJ010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6487
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.196"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            265..471
                     /locus_tag="OQQ48_003785"
     CDS             265..471
                     /locus_tag="OQQ48_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1954377.1"
                     /translation="MAEEKKGGVSVYISPEIVEVLKQRHKKNYEAGVAAGLDPLMTPE
                     PSIGSLVRSYLLAALGMHKNYGGE"
     gene            471..1526
                     /locus_tag="OQQ48_003786"
     CDS             471..1526
                     /locus_tag="OQQ48_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HCS1954378.1"
                     /translation="MAGKATALNTNQLFMYLNRGDIADFKFSPLFTTLFFPNVATFST
                     QNIMLDTLDIEEVTMSAFCSPMVGSQVQRDKGYETSTIKPGYMKPKHEIDPTKTIMRM
                     AGEDPAQLNDPTYRRMRLITGNMRRQINAIKARVEWLAVNAVTTGKNIIEGEGIERYE
                     IDWKIPEKNIIEQSDGKKWSEHDKDIYDPIYDIELYADQAGCPANVMIMGAEVWRTLR
                     SFKKFRELYDLSRGSESAAEMACKNLGEVVSFKGYLGDIALIVYSGKYTDSDGTEKYF
                     LEPDLLVLGNTNNKGLVAYGAIMDQEAVRTGATQNMYYPKNWIEDGDPAIEYVQTHSA
                     PQPVPADIRKFVTVKIG"
     gene            1538..1873
                     /locus_tag="OQQ48_003787"
     CDS             1538..1873
                     /locus_tag="OQQ48_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HCS1954379.1"
                     /translation="MDTPYIELFAGSQQVSTTLVHFAADAGVIQEFTPLMLVDNGEFK
                     PWDGQESGKAVYLTSYLVDTSKQKSAQCYKTGIFNIAAVNWPESVDTDAKKCAAFAGS
                     GVSVQPLAR"
     gene            1886..2299
                     /locus_tag="OQQ48_003788"
     CDS             1886..2299
                     /locus_tag="OQQ48_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HCS1954380.1"
                     /translation="MATNESIMALPLASKFKAEARAMADRGLSTYEAVYQLNKLEEQD
                     KPRADAIMALHEHKDYQPLLRAMANVPCIDVDTAKNILNMTIEQERPKVAPELTAAFE
                     NFMDMHSPQTVSAGMAYDGRNPGDDGDIDRILKTI"
     gene            2461..2895
                     /locus_tag="OQQ48_003789"
     CDS             2461..2895
                     /locus_tag="OQQ48_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1954381.1"
                     /translation="MIDKACFVSQQEIAEHFKVNRTTIRAWTKQGMPYLNADRGKSGG
                     YHIGHTLLWSSGKSRLEAIRYHVETSALEKIMFARLLSSERDEYSSEETEHRFDEGLQ
                     IYGYSPEDVSKARNKMAGFLAGWRHAVSVRRASMEQSADTEQ"
     gene            3176..3457
                     /locus_tag="OQQ48_003790"
     CDS             3176..3457
                     /locus_tag="OQQ48_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1954382.1"
                     /translation="MTEAEILGLIRRAGGISQQTDEQATQPDSVTAENYARVVAEVMR
                     RDGIQLNDVDMRNIRIRVLEMLAYNRRVALYRETEKITYHWKKPERLRR"
     gene            complement(4131..>6487)
                     /gene="yejO"
                     /locus_tag="OQQ48_003791"
     CDS             complement(4131..>6487)
                     /gene="yejO"
                     /locus_tag="OQQ48_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HCS1954383.1"
                     /translation="GGYQYIEMNGTAEYSVLNDGYQIVQMGGAANQTTLNNGVLQVYG
                     AANDPTIKGGRLIVEKDGITVLAAIEKGGLLEVKEGGLAIAVDQKAGGAIKASTRVME
                     AFGINRLGQFEIKNGIANNMLLENGGSLRVEENDFAYNTTVDSGGLLEVMDGGTATGV
                     DKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYELDDGSGLIVMEDTQAIDTILD
                     EHATMQSLGKDTGTKVQANAVYDLGRSDQNGSITYSSKAISENMVINNGRANVWAGTM
                     VNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGASFRTHGAVDTSKADVSLENSVW
                     AIIADITTTNQNTLLNLANLAMSDANVIMMDEPVTRSSVTASAENFITLTTNTLSGNG
                     NFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASPAAGDSLTLVTTGGGDAAFTLG
                     NAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTTDVLNMAAAQPLVFDAELDTVR
                     ERLGSVKSVSYDTAMWSSAINTRNNVTTDAGAGFEQTLTGLTLGIDSRFSREESSTIR
                     GLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNGAYVDGVVKVDRFANTIHGKMS
                     NGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAFTGFTTDGQDYTLSNGMRADVG
                     NTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEYADSNHVKVNDDGKFNNDVAGT
                     PGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQAGVVWTF"
BASE COUNT         1735 a         1605 c         1525 g         1622 t
ORIGIN      
        1 gatcagagac tgatccagca cacagcacac atagccggag caatccggct tttttgcgcc
       61 caaaaaaagc ccgataaggt cagagggttc ttatcgggct tttgcatatg aggtttttgg
      121 atgcactggc gttcgtgatc gggataatca tttcataatt tgcaacataa ctcaatatta
      181 ttgcataaaa tgcaattctg attataatca ggactggata aacatccagt cctgattttt
      241 ttagtcgaag aggaatttct tactatggct gaagagaaaa aaggcggtgt ttcggtgtac
      301 ataagccccg aaatcgtgga ggtgctcaag cagcgccaca aaaaaaacta tgaggctggc
      361 gtggcggctg gactggatcc gctgatgacg ccggagccgt cgataggttc acttgtacgc
      421 tcttatttac ttgcggcgct tgggatgcat aaaaattatg ggggtgaata atggcaggca
      481 aagcaacggc acttaacact aaccagcttt ttatgtacct gaatcgcggg gatattgcgg
      541 attttaaatt cagccctctg tttaccacgc tgtttttccc gaacgtggcg acattcagca
      601 cgcaaaacat catgctggat accctggaca ttgaagaagt caccatgtcg gcgttttgtt
      661 cgcctatggt gggtagccag gttcagcgcg ataaagggta cgaaaccagc acaatcaaac
      721 ctggctacat gaagccaaag cacgaaatcg atccaacgaa aaccatcatg cgcatggctg
      781 gagaagatcc ggcacagctt aacgacccta cctatcgccg tatgcgcctg attactggca
      841 acatgcgccg ccagataaac gccattaagg cacgcgtgga atggctggcg gtgaatgcgg
      901 taacgaccgg aaaaaacatc attgagggcg aaggcataga acgctatgaa atcgactgga
      961 agataccgga aaaaaacatc atagagcagt ccgacggcaa aaaatggtcc gagcatgata
     1021 aagatattta cgatccaatc tatgacatcg aactatacgc agatcaggca ggttgccccg
     1081 ccaacgtcat gattatgggc gctgaggtat ggcgcacgtt acgcagcttt aaaaaattcc
     1141 gtgaactgta cgatctttcc cgtggttcag aatccgccgc agaaatggcc tgtaaaaacc
     1201 tgggcgaagt ggtgagcttt aaaggctatc tgggcgatat tgctcttatt gtctattccg
     1261 gcaaatacac tgacagcgac ggcaccgaaa aatatttcct tgagcctgat ttgctggtcc
     1321 tgggcaacac caacaataaa gggctggtgg cctatggtgc gattatggat caggaagcgg
     1381 taagaacggg cgcaacgcaa aacatgtact acccgaaaaa ctggattgag gacggcgatc
     1441 cggcgattga gtacgtgcag acacacagcg caccgcagcc ggttccggca gatattcgca
     1501 aatttgttac cgtcaaaatt ggttaacggg ggattatatg gacactccat atattgagtt
     1561 atttgcaggc agtcagcagg tatcaacgac actggtacat tttgccgctg atgctggcgt
     1621 tattcaggaa tttaccccgc tgatgctggt ggacaatggc gagtttaagc cgtgggatgg
     1681 tcaggaatct ggcaaggctg tttatctgac ttcgtacctc gtggacacgt caaagcagaa
     1741 atcagcacag tgttacaaga cggggatatt caatattgcc gccgttaact ggcctgagag
     1801 cgtcgacacc gatgcgaaaa aatgcgccgc ctttgcgggt tctggcgtat ccgttcagcc
     1861 gctggcccga taagcagggg gaacgatggc aacgaatgaa agcatcatgg cgctaccgct
     1921 ggcgagtaaa tttaaagccg aagcgcgggc aatggctgac agaggtttat caacctacga
     1981 ggccgtatat caactcaaca aactggaaga gcaggacaag ccgcgcgctg atgcgattat
     2041 ggcgcttcat gagcataaag actatcagcc gctgttgcgt gcaatggcaa acgtgccttg
     2101 tatcgatgtt gatacggcta aaaacatcct gaacatgacc atagagcagg aacgcccgaa
     2161 ggttgcacca gagcttaccg cagcctttga aaactttatg gacatgcaca gcccacaaac
     2221 cgtatcagct ggcatggcat acgatggcag aaacccaggc gatgacggcg acatcgatcg
     2281 catactgaaa accatctgag acaaggccgg agaaatccgg cttttttgcg ggtccttcct
     2341 ggaattatgg cccgttacgg ggcggcgacc tcgcgggttt tcgctattta tgacgttttt
     2401 ccgtgaaggt gacaccacca ccacttgatt aatatttaac catgcagtta aggtaacatt
     2461 atgattgata aagcttgttt tgtaagtcag caggaaatag ctgaacattt caaggttaac
     2521 agaaccacta ttcgcgcatg gaccaaacag gggatgccgt atcttaatgc ggatcgcgga
     2581 aagtctggcg gttatcacat cgggcataca ttgctttggt cttcaggtaa aagccgtctt
     2641 gaggccatca gatatcacgt agaaaccagt gcgctggaaa aaattatgtt tgccaggctg
     2701 ctttcatctg agcgtgacga gtactccagt gaagaaacag aacatcgatt tgatgaaggt
     2761 ttgcagattt acggctattc accggaagat gtgagcaagg cacgaaataa aatggctggc
     2821 tttctggctg ggtggcgtca tgccgtaagc gttcgccgtg ccagcatgga gcaatcagcc
     2881 gatacagaac agtaaagccg ataacttgtt aatcactcgc agcaatgcat gtgatttgtg
     2941 cacactttcg gttaactggt tcgatgtccg gttttagtgt ctgttttttg cgcatgtccg
     3001 gttcatggaa agcatgtttt tatatttttc atatggttaa cttgtagaga aaccggacat
     3061 ggatcccgaa aaattttcat aaatagtgaa aacgcgcgag gtcgccgccc cgtaacgggt
     3121 ccataattcc aggaaggacc cgacgacacc agacaatcag aacgataggt gcacaatgac
     3181 agaagccgaa atactgggat taatccgccg tgctggtgga atcagccagc agactgacga
     3241 acaggccacg cagccggaca gcgtgacagc cgaaaattat gcgcgtgttg ttgctgaggt
     3301 gatgcgccgt gatggtatcc agcttaatga tgtggatatg cgcaacatac ggatccgcgt
     3361 tcttgaaatg ctggcctaca atcgccgcgt ggcactgtat cgggaaacgg agaaaattac
     3421 ataccactgg aagaagccgg agcggttgcg gcggtaactg gttgatattt tcgaaattcg
     3481 ccagcggcga acattgggga aaccagccta acccgttgat attttcggga acctcaaatt
     3541 gaggaggtcg gaaacataac ccgtcatatt ctggatgctg ataattacca cgcagcccat
     3601 taaggcctaa cccgttgatt ctttcggaaa cagccattgg cgtaatccgg cgcggtaact
     3661 tgctgaactt aaagcaaagc gcaaaattgc gctggctggt gggcatgatg ccgatatggt
     3721 gatccccata acggaaatat ccaccagcac ggcaacggca gaatagccac aacacagaaa
     3781 aaccacgaat atggggtttt tgttatggca tgggcatgat gaccgcccct gctctgaatg
     3841 gtggaaatca ccacgcagat catccaccag cttcgttttt ataaattaaa catccgggaa
     3901 aatccgaccc tgaattgtgc attttttcag caaatcattt tagttatacg tttggttata
     3961 cggtttaaaa gttgaatcat atacattatt ttaaatcatg aagttacacg ctcaattaaa
     4021 ctactctcgt gatcccaaga aaaaaccaac ctttacggtt ggttttttta tatctgcaat
     4081 taatttgata aacagaccgt gacacatcac agcctgttta ttttctgtta tcagaacgtc
     4141 cagaccactc ccgcctgagt attccacggc gattctaccc ctgcgccatt gccatagctg
     4201 actgacaaat gaccgcttaa cgtcggggta aacgatgacc ttatcccagc ctgataaacg
     4261 ccaggggttc cagccacatc attattaaat ttgccatcgt cattaacttt cacatggtta
     4321 gaatcggcgt attcctgacg tacggcggct ttcagccagg gttccagcgt cgtaccattt
     4381 tgcaggtcca tgtgatagct taccgccgtt cccgcttcag cgcgtaatat ccgggtattt
     4441 cccacatcag cgcgcatgcc gtttgataac gtgtagtcct gaccatctgt ggtaaagccg
     4501 gtaaaggcca gatagggtct aacactccac aatccgtcaa cccaacggaa cccgctctca
     4561 acatgagcac ccgcgccgtt actattgtaa tcgccaaacg ctgttgcccc attactcatc
     4621 ttgccatgga tggtgttggc aaaacggtca actttcacca ccccatcaac ataggcaccg
     4681 ttctgatgct cccaaccggc ataagccccc agggtatagc tatcgatatt accttttccg
     4741 ccgcgatcaa aaccaatatc agaatgagag taaccaaaga acaagccgcg aattgtactg
     4801 ctttcttcac gggagaaacg gctatcgata ccgagcgtca ggcccgtcaa tgtttgctca
     4861 aaaccagctc ccgcatcagt ggtcacgttg ttgcgggtgt taattgccga actccacatc
     4921 gccgtatcgt aactaacgct ttttacgcta ccaagacgct cacgcacggt gtccagttct
     4981 gcatcaaata ccagcggttg tgcggccgcc atattcagca catcagtggt tgaaggggta
     5041 atttgcgcgc gattctctgc caaactccag ctatggttgc cattatccag caaggtatat
     5101 tcatacgtac cgatatcaac aacgcctccg gcattgccca acgtaaatgc agcatcaccg
     5161 ccgcccgttg ttaccagtgt aaggctatct cctgctgccg ggctggcacc ggtgtccgtc
     5221 acgaatattt tgaaatcacc tgttgcctga ccggtgacgt tgagctgatc gctctgatga
     5281 ttagccatat cggtacgcat ataaaaattg ccgtttcccg acagggtatt ggtggtcaac
     5341 gtaatgaaat tttccgcact tgccgtcact gatgaacgag tcactggctc atccatcata
     5401 atcacatttg cgtcagacat cgcaaggttg gctaagttga ggagggtgtt ttggttcgtc
     5461 gtagtgatat cggcaatgat ggcccatacg ctattttcga gcgaaacgtc cgctttgctg
     5521 gtatccacgg caccatgcgt tctaaaagaa gcgccctcag aaaccactac cttacccacc
     5581 aacatagcgg gtgcataatt tatttgcggc ttcatgacct caagaatgcc atcattccct
     5641 ctgactgaca cgttaaccat tgtgccagcc cagacattag cgcggccatt gttgataacc
     5701 atattttcag agatggcttt agaggaatac gtgatacttc cattctggtc tgatcgaccg
     5761 agatcatata ccgcatttgc ctgcactttc gtaccagtat cctttcccag cgattgcata
     5821 gtggcatgct catcaaggat agtatcaatg gcctgcgtgt cctccataac aatgagcccg
     5881 gaaccatcat ccagttcata attttttgac acaccatctt ttatactaaa ttggccttta
     5941 ctgtttgtac cactcacttc cagcgcattc gttgagacaa ttaattttcc gcctgctttt
     6001 ttatcaacgc cagttgcagt cccgccatcc ataacctcca gtaagccgcc actatctaca
     6061 gtagtattat aagcgaagtc attttcttca actcgcaaac ttccgccgtt ttccaacagc
     6121 atattgttag caataccatt cttgatttcg aactgaccga gacggtttat tccgaatgcc
     6181 tccatgaccc gcgtgcttgc tttaatagca ccgcctgctt tctgatctac cgcaatcgct
     6241 aatcccccct ctttaacctc cagtaatcct cccttttcga tagcggcaag gacggtaatc
     6301 ccatcttttt caacgattaa gcgcccgcct ttaatcgtgg gatcattcgc tgcgccataa
     6361 acctgtagca caccattatt gagggtagtc tggtttgccg cgccacccat ttgaacaatt
     6421 tgataaccgt catttaatac tgagtattct gcggtaccat tcatttcaat gtactgatac
     6481 cctccgt
//
LOCUS       DAJVUJ010000197         6453 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.197, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000197 DAJVUJ010000000
VERSION     DAJVUJ010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6453
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.197"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            186..2102
                     /gene="dnaK"
                     /locus_tag="OQQ48_003792"
     CDS             186..2102
                     /gene="dnaK"
                     /locus_tag="OQQ48_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCS1954384.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            2191..3321
                     /gene="dnaJ"
                     /locus_tag="OQQ48_003793"
     CDS             2191..3321
                     /gene="dnaJ"
                     /locus_tag="OQQ48_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCS1954385.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGQHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(3425..3577)
                     /gene="mokC"
                     /locus_tag="OQQ48_003794"
     CDS             complement(3425..3577)
                     /gene="mokC"
                     /locus_tag="OQQ48_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HCS1954386.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            4163..5329
                     /gene="nhaA"
                     /locus_tag="OQQ48_003795"
     CDS             4163..5329
                     /gene="nhaA"
                     /locus_tag="OQQ48_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HCS1954387.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMMMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            5395..6294
                     /gene="nhaR"
                     /locus_tag="OQQ48_003796"
     CDS             5395..6294
                     /gene="nhaR"
                     /locus_tag="OQQ48_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HCS1954388.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(6332..>6453)
                     /locus_tag="OQQ48_003797"
     CDS             complement(6332..>6453)
                     /locus_tag="OQQ48_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HCS1954389.1"
                     /translation="QHPHEGSNQIPLFIRPRTYGNNVSSGQIMSRVKIVVMYN"
BASE COUNT         1632 a         1531 c         1701 g         1589 t
ORIGIN      
        1 ctgcgcaaaa gcacaaaaaa tttttgcatc tcccccttga tgacgtgggt tacgacccca
       61 tttagtagtc aaccgcagtg agtgagtctg caaaaaaatg aaattgggca gttgaaacca
      121 gacatttcgc ccctattaca gactcacaac cacatgatga ccgaatatat agtggagacg
      181 tttagatggg taaaataatt ggtatcgacc tgggtactac caactcttgt gtagcgatta
      241 tggatggcac cactcctcgc gtgctggaga acgccgaagg cgatcgcacc acgccttcta
      301 ttattgccta tacccaggat ggtgaaactc tggttggtca gccggctaaa cgtcaggcag
      361 tgacgaaccc gcaaaacacc ctgtttgcga ttaaacgcct gattggccgc cgcttccagg
      421 acgaagaagt acagcgtgat gtttccatca tgccgttcaa aattattgct gctgataacg
      481 gcgacgcatg ggtcgaagtt aaaggccaga aaatggcacc gccgcagatt tctgctgaag
      541 tgctgaaaaa aatgaagaaa accgctgaag attacttggg tgaaccggta actgaagctg
      601 ttatcaccgt accggcatac tttaacgatg ctcagcgtca ggcaaccaaa gacgcaggcc
      661 gtatcgctgg tctggaagta aaacgtatca tcaacgaacc gaccgcagct gcgctggctt
      721 acggtctgga caaaggtact ggcaaccgta ctatcgcggt ttatgacctg gggggtggta
      781 ctttcgatat ttctattatc gaaatcgacg aagttgatgg cgaaaaaacc ttcgaagttc
      841 tggctaccaa cggtgatacc cacctgggtg gtgaagactt cgacagccgt ctgatcaact
      901 acctggttga agaattcaag aaagatcagg gcattgacct gcgcaacgat ccgctggcaa
      961 tgcagcgcct gaaagaagcg gcagaaaaag cgaaaatcga actgtcttcc gctcagcaga
     1021 ccgacgttaa cctgccgtac atcactgcag atgcgaccgg tccgaaacac atgaacatca
     1081 aagtgactcg tgcgaaactg gaaagcttgg ttgaagatct ggtaaaccgt tccattgagc
     1141 cgctgaaagt tgcactgcag gacgctggcc tgtccgtatc tgatatcgac gacgttatcc
     1201 tcgttggtgg tcagactcgt atgccaatgg ttcagaagaa agttgctgag ttctttggta
     1261 aagagccgcg taaagacgtt aacccggacg aagctgtagc aatcggtgct gctgttcagg
     1321 gtggtgttct gactggtgac gtgaaagacg tactgctgct ggacgttacc ccgctgtctc
     1381 tgggtatcga aaccatgggc ggtgtgatga cgacgctgat cgcgaaaaac accactatcc
     1441 cgaccaagca cagccaggtg ttctctaccg ctgaagacaa ccagtctgcg gtaaccatcc
     1501 atgtgctgca gggtgaacgt aaacgtgcgg ctgataacaa atctctgggt cagttcaacc
     1561 tggatggtat caacccggca ccgcgcggca tgccgcagat cgaagttacc ttcgatatcg
     1621 atgctgacgg tatcctgcac gtttccgcga aagataaaaa cagcggtaaa gagcagaaga
     1681 tcaccatcaa ggcttcttct ggtctgaacg aagatgaaat ccagaaaatg gtacgcgacg
     1741 cagaagctaa cgccgaagct gaccgtaagt ttgaagagct ggtacagact cgcaaccagg
     1801 gcgaccatct gctgcacagc acccgtaagc aggttgaaga agcaggcgac aaactgccgg
     1861 ctgacgacaa aactgctatc gagtctgcac tgactgcact ggaaactgct ctgaaaggtg
     1921 aagacaaagc cgctatcgaa gcgaaaatgc aggagctggc acaggtttcc cagaaactga
     1981 tggaaatcgc ccagcagcaa catgcccagc agcagactgc cggtgctgat gcttctgcaa
     2041 acaacgcgaa agatgacgat gttgtcgacg ctgaatttga agaagtcaaa gacaaaaaat
     2101 aatcgcccta taaacgggta attatactga cacgggcgaa ggggaatttc ctctccgccc
     2161 gtgcattcat ctaggggcaa tttaaaaaag atggctaagc aagattatta cgagatttta
     2221 ggcgtttcca aaacagcgga agagcgtgaa atcaaaaagg cctacaaacg cctggccatg
     2281 aaataccacc cggaccgtaa ccagggtgac aaagaggccg aggcgaaatt taaagagatc
     2341 aaggaagctt atgaagttct gaccgactcg caaaaacgtg cggcatacga tcagtatggt
     2401 catgctgcgt ttgagcaagg cggcatgggc ggcggcggtt ttggcggcgg cgcagacttc
     2461 agcgatattt ttggtgacgt tttcggcgat atttttggcg gcggacgtgg tcgtcaacgt
     2521 gcggcgcgcg gtgctgattt acgctataac atggagctca ccctcgaaga agctgtacgt
     2581 ggcgtgacca aagagatccg cattccgact ctggaagagt gtgacgtttg ccacggtagc
     2641 ggtgcaaaac caggtacaca gccgcagacc tgtccgacct gtcatggttc tggtcaggtg
     2701 cagatgcgtc agggtttctt tgccgtgcag cagacctgtc cacactgtca gggccgcggt
     2761 acgctgatca aagatccgtg caacaaatgt catggtcatg gtcgtgttga gcgcagcaaa
     2821 acgctgtccg ttaaaatccc ggcaggggtg gacactggag accgcatccg tcttgcgggc
     2881 gaaggtgaag cgggccagca tggcgcaccg gcaggcgatc tgtacgttca ggttcaggtt
     2941 aaacagcacc cgattttcga gcgtgaaggc aacaacctgt attgcgaagt cccgatcaac
     3001 ttcgctatgg cggcgctggg tggcgaaatc gaagtaccga cccttgatgg tcgcgtcaaa
     3061 ctgaaagtgc ctggcgaaac ccagaccggt aagcttttcc gtatgcgcgg taaaggcgtc
     3121 aagtctgtcc gcggtggcgc acagggtgat ttgctgtgcc gcgttgtcgt cgaaacaccg
     3181 gtaggcctga acgagaagca gaaacagctg ctgcaagagc tgcaagaaag cttcggtggc
     3241 ccaaccggcg agcacaacag cccgcgctca aagagcttct ttgatggtgt gaagaagttt
     3301 tttgacgacc tgacccgcta acctccccaa aagcctgccc gtgggcaggc ctgggtaaaa
     3361 atagggtgcg ttgaagatat gcgagcacct gtaaagtggc ggggatcact ccccgccgtt
     3421 gctcttactc ggattcgtaa gccgtgaaaa cagcaacctc cgtctggcca gttcggatgt
     3481 gaacctcaca gaggtctttt ctcgttacca gcgccgccac tacggcggtg atacagatga
     3541 cgatcagggc gacaatcatc gccttatgct gcttcattgc tctcttctcc ttgaccttac
     3601 ggtcagtaag aggcactcta catgtgttca gcatataggg ggcctcgggt tgatggtaaa
     3661 atatcactcg gggcttttct ctatctgccg ttcagctaat gcctgagaca gacagcctca
     3721 agcacccgcc gctattatat cgctctcttt aacccatttt gttttatcga ttctaatcct
     3781 gaagacgcct cgcatttttg tggcgtaatt ttttaatgat ttaattattt aactttaatt
     3841 tatctcttta tcgcaattat tgacgacaag ctggattatt tttgaaatat tgtcctaaca
     3901 aataacgccg actgacaaga aattaattat tacttttcct aattaatccc tcagaaatcc
     3961 tcaccttaag ctatgattat ctgagcttag ggtcactcgt cagcgcttac agccgtcaaa
     4021 aactcatctc accgctgatg gcgaaaattc tacaaaggct cgtaaaaaac gaattattcc
     4081 tacactataa tctgatttta acgatgattc gtgcggggta aaatagtaaa aacgatttat
     4141 tcacctgaaa gagaaataaa aagtgaaaca tctgcatcga ttctttagca gtgatgcctc
     4201 gggaggcatt attcttatca ttgccgctat cctggcgatg atgatggcca acagcggcgc
     4261 aaccagtgga tggtatcacg actttctgga gacgccggtt cagctccggg ttggttcact
     4321 cgaaatcaac aaaaacatgc tgttatggat aaatgacgcg ctgatggcgg tatttttcct
     4381 gttagtcggt ctggaagtta aacgtgaact gatgcaagga tcgctagcca gcttacgcca
     4441 ggccgcattt ccagttatcg ccgctattgg tgggatgatt gtgccggcat tactctatct
     4501 ggcctttaac tatgccgatc cgattacccg cgaagggtgg gcgatcccgg cggctactga
     4561 cattgccttt gcacttggag tgctggcgct gttgggaagt cgtgttccgt tagcactgaa
     4621 gatctttttg atggctctgg ctattatcga cgatcttggg gccatcatta tcatcgcatt
     4681 gttctacact aatgacttat cgatggcctc tcttggcgtc gcggctgtag caattgcggt
     4741 actcgcggta ttgaatctgt gtggtgtacg ccgcacgggc gtctatattc tggttggcgt
     4801 agtgttgtgg actgcggtgt tgaaatcggg ggttcacgca accttggcgg gggtaattgt
     4861 cggcttcttt attcctttga aagagaagca tgggcgttct ccagcgaagc gactggagca
     4921 tgtgttacac ccgtgggtgg cgtatctgat tttgccgctg tttgcatttg ctaatgctgg
     4981 cgtttcactg caaggcgtca cgctggatgg cttgacctcc attctgccat tggggatcat
     5041 cgctggcttg ctgattggca aaccgctggg gattagtctg ttctgctggt tggcgctgcg
     5101 tttgaaactg gcgcatctgc ctgagggaac gacttatcag caaattatgg cggtggggat
     5161 cctgtgcggt atcggtttta ctatgtctat ctttattgcc agcctggcct ttggtagcgt
     5221 agatccagaa ctgattaact gggcgaaact cggtatcctg gtcggttcta tctcttcggc
     5281 ggtaattgga tacagctggt tacgcgttcg tttgcgtcca tcagtgtgac aggacggttt
     5341 accggggagc cataaacggc tcccttttca ttgttatcag ggagagaaat gagcatgtct
     5401 catatcaatt acaaccactt gtattacttc tggcatgtct ataaagaagg ttccgtggtt
     5461 ggcgcagcgg aggcgcttta tttaactcca caaaccatta ccggacagat ccgggctctg
     5521 gaagagcgcc tgcaaggcaa attatttaag cgcaagggac gtggtctcga acccagcgag
     5581 ctgggagaac tggtctatcg ctatgccgat aaaatgttca ccttaagcca ggaaatgctg
     5641 gatattgtga actatcgcaa agaatccaat ttattgtttg acgttggcgt ggctgatgca
     5701 ctttccaaac gcctggtcag tagcgtgctg aatgctgcag tggtagaagg cgagccgatt
     5761 catcttcgct gcttcgaatc cacccacgaa atgctactgg agcaattaag ccagcataaa
     5821 ctggatatga tcatttccga ctgtccgata gactctacgc agcaggaagg cctgttctcc
     5881 gtgagaattg gcgaatgtgg cgtgagtttc tggtgtacaa atccaccacc agaaaaaccg
     5941 ttcccggctt gtctggaaga acggcgactt ttgattcctg ggcgacgttc aatgttaggg
     6001 cgcaaattgc ttaactggtt taactcccag ggattaaacg tagaaatcct cggcgagttt
     6061 gatgatgccg cgttgatgaa agcttttggt gcaatgcaca atgcaatctt cgttgcccca
     6121 acgctttatg catatgactt ttatgccgat aaaactgtcg tagaaattgg tcgcgtcgag
     6181 aatgtgatgg aagagtacca tgctattttt gctgagcgga tgattcagca cccggcggta
     6241 cagcgaatct gcaatacgga ttattctgcg ctttttagtc cagcggcgcg ttaatcggca
     6301 gctcccccaa agttaaagtg gaggagatag attagttgta cataaccacg attttgactc
     6361 ggctcattat ttgcccgctt gagacattat ttccatatgt acgcgggcga ataaatagag
     6421 gaatctgatt acttccttca tggggatgct gaa
//
LOCUS       DAJVUJ010000198         6379 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.198, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000198 DAJVUJ010000000
VERSION     DAJVUJ010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6379
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.198"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(<1..205)
                     /locus_tag="OQQ48_003798"
     CDS             complement(<1..205)
                     /locus_tag="OQQ48_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HCS1954390.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRD"
     gene            684..2162
                     /locus_tag="OQQ48_003799"
     CDS             684..2162
                     /locus_tag="OQQ48_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCS1954391.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IEHWCITFYICIPVVWDRFILALLPMSSKYCVSIVGPETCAS"
     gene            2045..2461
                     /locus_tag="OQQ48_003800"
     CDS             2045..2461
                     /locus_tag="OQQ48_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004993937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial regulatory, Fis family protein"
                     /protein_id="HCS1954392.1"
                     /translation="MHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLME
                     YLVNVVPSGEVIDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMI
                     REALSRHNSKKQVADELGIGIATLYRKIKKYELLNT"
     gene            complement(2501..2980)
                     /gene="xdhC"
                     /locus_tag="OQQ48_003801"
     CDS             complement(2501..2980)
                     /gene="xdhC"
                     /locus_tag="OQQ48_003801"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HCS1954393.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(2977..3855)
                     /gene="xdhB"
                     /locus_tag="OQQ48_003802"
     CDS             complement(2977..3855)
                     /gene="xdhB"
                     /locus_tag="OQQ48_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HCS1954394.1"
                     /translation="MFDFASYHRAATLADAINLLANNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAAMSIAG
                     LQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQMMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(3866..6163)
                     /gene="xdhA"
                     /locus_tag="OQQ48_003803"
     CDS             complement(3866..6163)
                     /gene="xdhA"
                     /locus_tag="OQQ48_003803"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HCS1954395.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAIAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGTFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
BASE COUNT         1592 a         1509 c         1611 g         1667 t
ORIGIN      
        1 tgtcgcggaa tacggaaata cctaatccac tattaaagac tttggttgag atgttttcag
       61 cacgcagtgc ttttaatgct gcggcaacgt ccagtacttg tttcagttca tctggcgtct
      121 gttcccacgt taacaaaaaa tctttctcgt gaaggtgaga ggtcagcgaa ttgatatcct
      181 taatcagctc attaacagtt ttcatcataa agtcccggta gtggaatgac aggcaaagtg
      241 gtttatttca ctgtgacttg ccaaagtgat tggtgttttc acagctatac ctgggactaa
      301 caataagtgt gccagaatta tccgcgcggc tgcaaaagac taatttgacg gataatgtgt
      361 ggggttgatc acaaactgat gccagcttct ggttgtttgt aaaatacgaa aacgtttggc
      421 tttttttatc gcgttattta tttgctgcta agtatcagaa ataagataaa ggggcaggct
      481 atctctcttc agtttgataa actcaccgcc gtaattctca tattgatata gaaatgtgat
      541 atctcttcca taatttcatt agaaaatgat aaaaatctta tgtttttatc aaagccaatt
      601 tcgcctgagt taatatcatc atgatctgtg aaccatcaac gtcttcaatg atcgactttt
      661 aagtaagtct ggaaggtaaa gctatggagc ttgctactac gcagtcagta ttgatgcaaa
      721 ttcaaccgac aattcagcgt tttgccagaa tgcttgccag cgttttgcag cttgaggttg
      781 agatcgttga tgaaaacttg tgtcgcgtcg ccggaacggg cgcgtatggg aagtttcttg
      841 gtcgccagtt gagcggcaac tcacgcctgc tccgccacgt cctggaaacg aaaactgaaa
      901 aagttgtgac acagtctcgc ttcgatcccc tttgcgaagg ttgcgatagt aaagaaaatt
      961 gccgtgaaaa agcatttctg ggtacgcctg tcattttaca ggatcgttgt gttggggtga
     1021 taagtttgat tgccgttacc cacgagcaac aagagcatat cagtgataat ttacgcgaat
     1081 tttctgatta tgttcgccat atatccacca tttttgtttc gaaacttctg gaggatcagg
     1141 ggccaggaga taacatcagt aaaatattcg cgaccatgat cgataatatg gatcagggcg
     1201 tattagttgt tgatgatgaa agtcgggttc agtttgttaa tcagactgcc ttaaaaacac
     1261 ttggtgttgt acaaaataat attattggga aacctatccg tttcagacca ttaacatttg
     1321 agagtaattt tactcatgga catatgcagc atattgtttc gtgggacgat aaaagtgaat
     1381 taatcattgg tcaattgcat aacattcagg gccgacaatt atttttaatg gcatttcacc
     1441 aatcgcatac cagtttttct gtagcaaatg cacctgatga accacatatt gaacaattgg
     1501 ttggcgagtg ccgtgttatg cggcaattaa aacgactcat tagccgtatt gcacccagcc
     1561 catccagcgt tatggtggtt ggtgaaagcg gcacgggtaa agaagtcgtc gcccgagcaa
     1621 tccataagtt gagcggaaga cggaataaac cctttattgc tatcaactgt gccgcgattc
     1681 cggagcagct tctggaaagc gaactgttcg gttatgttaa aggcgcattt actggcgctt
     1741 ctgccaacgg taaaacaggg ttgattcagg cggcgaatac gggcacgctg tttctcgatg
     1801 aaataggtga tatgccatta atgttgcagg ctaaattact gcgcgctatt gaggcgcgtg
     1861 aaattctgcc gattggtgcc agtagcccaa tacaagtcga cattcgcatc atttctgcaa
     1921 ctaatcagaa tttggcccag ttcattgccg aaggtaaatt ccgcgaagat ctcttctacc
     1981 gacttaatgt tatcccgata actctgccac cgctgcgtga acgtcaggaa gatattgaac
     2041 attggtgcat tactttttac atctgcatac ccgtcgtctg ggatcggttt atcctggcat
     2101 tgctcccgat gtcgtcgaaa tattgcgtaa gcatcgttgg cccggaaacc tgcgcgagtt
     2161 aagcaatttg atggaatatc tggttaacgt agttccttca ggtgaagtta tcgacagcac
     2221 gctattgccg ccaaatctgc tgaataatgg cacaacggag caaagtgatg taacagaggt
     2281 cagtgaggcg cacctgtcac tcgatgatgc gggcggcacg gcgctggagg agatggaaaa
     2341 gcaaatgatc cgcgaggcgc tttcacgtca taacagcaag aagcaagttg ctgatgaact
     2401 gggcatcggc attgctacgc tctatcgcaa gattaagaaa tatgagttgt taaacacata
     2461 acctcagtga atccggcctg aattcaggcc ggatatcctt ttacttcgtt ttctcgcaat
     2521 ccagaactgt atttacaatc atctgatacc ccgtgcagcg acaaagattt cccgccagtc
     2581 cgcgacgaat ttccgtaatg gttaatggct tctcgcgtgg tttcgccagc attgccgtgg
     2641 tagccataat caggccaggc gtacaaaacc cgcactgcac tgcgccggat ttcgcataag
     2701 cctgctgaac atgagaaagt tttccgcctt tcgcttcacc ttccagcgtg cggatctctt
     2761 ttccttcagc ccaggcggca aggtataagc aactgtctat tgctgtgccg tcgaccaaca
     2821 ccgtacaggc accacattca cccacgcagc acccttgttt gacacttagc agtccttgtt
     2881 cgcggagtaa ttccgagagc ggcgtgcctg gtgcggcgtg aagctgaaaa ggcatcccgt
     2941 taatggtgca ttcgatggta attgtttcgc tgtgattcat tgcaattttc cccccgccgc
     3001 ggcgacggct tcgctaatca cttttttggt catcatctgg atgagatgca gacgaaactc
     3061 tttactggcc cgccatgaag aacgcggggc gacatcttgc aggacagatt cgctgatagc
     3121 ttccagcgtt tgcaggttta atggcgcatt ttgtgcagtc tgttcggcat gttggcagcg
     3181 aatcggcgtt ggcgcggcaa caccaaatgc caggcgtaat tcgctgaaat tgccgttatc
     3241 cagtcggcaa tgtgcggcgc agccaatcgt tgaaatatcc attgcgtcgc gcatggcata
     3301 tttaaaatgc gcgctgcccg cgtgttcttt cggctgtggc ggaaaatgaa aggcgacgag
     3361 gatttcgtca tgctcaagag acactttgcc cggcccggtg tgaaagccat taatcgggac
     3421 gaaacgaaca ccgcgcgggg agtggatctc cagtttcgcg tcataaatta gcgttggcgt
     3481 ggcagaatct gcgctggtgg caccgttgca aatatttcca ccgtaggtag cgacgttacg
     3541 gatctgcagt ccagcaatgg acatggccgc agcacataac gccgggagat gacgttgagt
     3601 tataggatct tctattagct gggtaaatgt cgttgcagag ccgatacgta gcgagccatc
     3661 ttccgccagc gtaattcccc gcagctccgc cagattatga atatcaacaa tatggcgata
     3721 acggtcattg tgatggtgga gctgaatcag tacgtcagtg ccaccggcga gcagtttggc
     3781 ctgcgggttg ttagccagca ggttgatggc atcggcaagg gttgctgcgc gatggtaaga
     3841 agcaaaatca aacatgatgt tatcctcaaa tcaatcctgc cagatggaac tcttcatata
     3901 accgttttgg cgtcagcggc agtgtattga ttgcaacacc ggtagccatc ttcaccgcgt
     3961 tacgaatagc agcggcaaca ggaattattg gtggctcacc cagtgactta tgtccgtatg
     4021 cggattgcgg ctcattgatt tcgacgaacg cgctttccag ttgtggcaga tccggcatgg
     4081 tcggcatttt gtaatccagc agattggggt tacggaccac gccgctttta gcatcgatga
     4141 tcatctcttc aaatagcgcc cagccaatgc ccattcccat tccgccgtgt acctgacctt
     4201 ctgccagcag tggattaaga atatgccctg aatcatgaac gttgaggatg cggttgatgg
     4261 tgactttgca cagcgcaata tcgaccgtca gatcaacaaa ggtacagcca aacgccggtg
     4321 ggttagtggt ggttttgatg gagctttcag cagagagctg cccgccgcgt tcagggtggt
     4381 agaaagcgtc catcgccaaa tcttttaacg acattaacgg ctcttccggt cgttcaacca
     4441 gcacgatatg gccttttatc agggtcagat tcatcgctga ctgatgtagc atgactgcgg
     4501 cgtgagcgat gattttctct tttaataata gtgccgcact gcgcagcgca ggcgcggcaa
     4561 catagctctg gcgtgaggca aatgtgccgg gatcgaacgg cgtaacgtcg gtatcttgag
     4621 ttgaaataac gcgaacgtcg ctgaccggaa cccccacggt ttctgccacc atttgcgaga
     4681 agacggtgtc ggcaccctga ccgatttccg tcgcgccgct ttgcacgttg atggttccat
     4741 cctgattcat cagaaggcgc gcgcctgcta tttctacgcc gacaggccag gtgttagagg
     4801 tgtagctaaa acaggcgacg ccaacgccgc ggcgcaaatt gccttgctgg ttctggcatt
     4861 ctgcacggcg tttttcccat tcaaagattt tccggccttt ttcaagacac tccggcaacc
     4921 ctgcgctgta aatacgtttg cccgtgagcg gattagcatc tccttcgcgg gcggcgttgc
     4981 gtaaacgaat ttcaacagga tcaataccta acgctgtcgc ggcgtcatca agcatagact
     5041 caacggcaaa tacgacttgt ggcgcgccat aaccacgcat cgcaccagcc gaggggaggt
     5101 tggtatagca ggtctttgaa ctgtaagcgt aggcacaacg aggataaagg taagcgactt
     5161 tattcccccc cgcagaagcg atggagtgcc cgtgagatgc ataagcgccg gtgttagaca
     5221 gaacatccag actataacct ttcaatgttc cgtcgcggtt cacgcccatt tgcccgtcaa
     5281 tggtaaaagc gtggcgggta cgggttgcga ggaaacactc ttcacggcta agggaaactt
     5341 tcaccggaat gccgccaagc ttgctggtca ggaatgccgc cattggctct tccagtacat
     5401 cctgtttatt accaaaaccg ccaccgacaa atggtttgat gactcgtacg catgaccagg
     5461 gaatatccag cgcctgacca accacgcggc gaacaatgtg cgggatctgg gtgctggaaa
     5521 cgatggtaat tcgcgagtca tcctccatcc acgccagcga tgttacgctt tccatgtgac
     5581 aatgttgaat aacgggggtc tgatagtgcc cctgtacctg gtagtcggcg gcatcgattg
     5641 tttgttggac attacccgtc gacatcgtgc tttgtttcag taaattgcca ccgttatgga
     5701 ttggtgcagc gtcttctgcc agcgccgctt ctggcgtggt gataacgggt aattcttgcc
     5761 actcaatgct gaccaattgc gccgcttttt ctgccgtgag ttcatcgcgg gccacgacga
     5821 tggcaatggc gtcaccatga tgacgaacat ggcgagttag cagtgcgcga tcggcggtat
     5881 cgcgcttgtt ttcgtcaagt gtccaggcat gcccagctgt agcgaatgga atatcaggca
     5941 catcttccca ggtaaaaatc gccagtacgc ccggcaaact tctggcttgt tcatcattaa
     6001 tacttacggc ataaccatgt gcgatagggc tacgtacata tttcgcataa cacatgcccg
     6061 ccataacata atcgtcagta tatcgtgccc gcccggtgac cttagcaatg gcatcgacgc
     6121 gcatgcatga ttcacccgta gcggttgctt cccgcgcttc catagaaatc ccctcgattt
     6181 gaacatttgg ttcttatggc aaagatttag tctaagcagg caagatttac gccagaaacg
     6241 acgataagcg ttaggggagg taggaagtgt gatgacgtta aaaaaacaca gatatgatac
     6301 gaaacaggag tctgaatagg ggcagtgata cgtatcaata tgagtggcga taaaatcaat
     6361 gtgataaatt gggattatt
//
LOCUS       DAJVUJ010000199         6338 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.199, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000199 DAJVUJ010000000
VERSION     DAJVUJ010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6338
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.199"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            31..153
                     /locus_tag="OQQ48_003804"
     CDS             31..153
                     /locus_tag="OQQ48_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001124879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HCS1954396.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDT"
     gene            169..1014
                     /locus_tag="OQQ48_003805"
     CDS             169..1014
                     /locus_tag="OQQ48_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCS1954397.1"
                     /translation="MRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDA
                     IILSAVSENGSSRTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLSN
                     AAADYFIANKIDQPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDK
                     AISVGEKLIISTPDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELE
                     NNQVLKAVVDISGKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATH
                     VDGLP"
     gene            1037..2548
                     /locus_tag="OQQ48_003806"
     CDS             1037..2548
                     /locus_tag="OQQ48_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCS1954398.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            2573..3571
                     /locus_tag="OQQ48_003807"
     CDS             2573..3571
                     /locus_tag="OQQ48_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCS1954399.1"
                     /translation="MSASSLSLPLGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            3604..4698
                     /locus_tag="OQQ48_003808"
     CDS             3604..4698
                     /locus_tag="OQQ48_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCS1954400.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALRSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            4710..5582
                     /locus_tag="OQQ48_003809"
     CDS             4710..5582
                     /locus_tag="OQQ48_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HCS1954401.1"
                     /translation="MTIYTLSHGPLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(5630..6052)
                     /locus_tag="OQQ48_003810"
     CDS             complement(5630..6052)
                     /locus_tag="OQQ48_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCS1954402.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIILGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(6149..>6338)
                     /locus_tag="OQQ48_003811"
     CDS             complement(6149..>6338)
                     /locus_tag="OQQ48_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phenoxybenzoate dioxygenase"
                     /protein_id="HCS1954403.1"
                     /translation="WLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSG
                     KTFDAKLLIDENIALKSL"
BASE COUNT         1467 a         1515 c         1836 g         1520 t
ORIGIN      
        1 gttgttaata aaaaccctac aggaggcact atgcctaaaa aaatgagaac aacccgtaat
       61 ctattattaa tggctacgct attaggtagc gcactatttg ccagggctgc ggataaagaa
      121 atgaccattg gtgcaatata ccttgatacc tagggatatt acgctggagt gcgtcagggc
      181 gttcaggatg cggcaaaaga ttcttcagta caggtacagt taattgaaac taacgcccag
      241 ggtgatattt cgaaagaaag tacctttgtt gataccctcg tggcgcgtaa tgtcgatgcc
      301 attattttat cggcagtgtc tgaaaatggc agtagccgta ccgttcgtcg cgccagtgaa
      361 gcgggtattc cggtgatttg ctacaacacc tgtattaatc aaaagggtgt cgataaatat
      421 gtctcggcgt atctggtcgg cgatccactg gaatttggta aaaaactgag taacgctgcc
      481 gccgattatt ttattgccaa taaaattgac cagccgaaaa ttgccgtcat caattgcgaa
      541 gcctttgaag tttgtgtgca gcgacgtaaa ggatttgaag aagtattaaa atcccgcgtt
      601 cccggcgcgc aaattgtcgc taatcaggaa gggactgttt tagataaagc gatttccgtt
      661 ggtgaaaaac tgattatctc cacgccggat ctcaacgcca ttatggggga gtcgggcggt
      721 gcgacactcg gcgcggtaaa agcggtacgt aatcaaaatc aggccggaaa aattgctgtt
      781 ttcggttcgg atatgacaac cgaaattgct caggagctgg aaaacaatca ggtgctgaaa
      841 gcggtagtgg atatttccgg taagaaaatg ggcaatgctg ttttcgcgca aacattgaag
      901 gttatcaata aacaagccga cggtgaaaaa gtgattcagg tgccaatcga tctctatacc
      961 aaaacggaag atggtaaaca gtggctggca acgcacgttg atggtctgcc ctaatcgctg
     1021 attcgcagag gtccctatgt tcacggcaac agaggcagtc ccggtagcaa aagtggtggc
     1081 aggaaataag cgttaccccg gcgtcgttgc gctggataac gttaacttca cgctcaataa
     1141 aggcgaagtt cgtgcgctgt taggtaaaaa cggcgcgggc aaatcgactc tcattcgaat
     1201 gcttaccggc agcgaacgcc cggatagcgg tgatatctgg attggcgaga cgcgactgga
     1261 aggtgacgaa gctacgttga ctcgccgtgc cgctgaactg ggggttcgtg cggtttatca
     1321 ggaattaagt ctggtggaag ggctgacggt ggcagaaaac ctctgccttg gtcaatggcc
     1381 tcgccgcaac ggcatgattg attacctgca aatggcgcag gatgcccaac gttgcttaca
     1441 ggcgctgggc gttgacgtta gtcctgaaca acttgtttca acgctaagcc cggcgcaaaa
     1501 gcaactggtg gaaattgcac gggtgatgaa gggcgagccg cgcgtggtca ttcttgatga
     1561 acccaccagc tcgcttgcca gtgcagaagt tgaactggtg atcagtgcgg tgaaaaagat
     1621 gtcggcactg ggcgtggcgg tgatttatgt cagccaccgg atggaagaaa tccgccgcat
     1681 tgcctcctgt gccaccgtta tgcgcgatgg tcaggtggcg ggcgatgtga tgctcgaaaa
     1741 cacctccacg catcatattg tgtcgctaat gctagggcgt gatcacgttg atattgcgcc
     1801 ggttgcccct caggaaattg tggatcaggc cgtactggaa gtccgtgcgt tacgccataa
     1861 gcccaagctg gaggatatca gctttacgtt acgtcgcggc gaagtgcttg gcattgctgg
     1921 cctgctgggg gcagggcgca gtgaattgct gaaggcgatt gttgggctgg agacgtatga
     1981 acagggcgaa attgttatca acggcgagaa aatcacgcgc cccgattacg gcgacatgct
     2041 gaaacgcggc attggctata cgccagaaaa ccgcaaagaa gcggggatca ttccctggtt
     2101 gggcgttgac gaaaatacag tgctgaccaa tcggcaaaaa atcagcgcca acggagtgct
     2161 gcaatggtcc accatccgcc gcctgaccga agaggtgatg cagcggatga cggtcaaggc
     2221 cgccagtagc gaaacgccca tcggcacgct ttctggtggc aatcagcaaa aagtggtgat
     2281 cggtcgttgg gtctatgccg ccagccagat tttgttgctc gacgagccaa cgcgcggcgt
     2341 cgatatcgaa gccaaacagc agatttaccg tattgtccgt gagctggctg ccgaaggaaa
     2401 aagcgtggtg tttatctcca gtgaagtgga ggagttgccg ctggtgtgcg accgcatcct
     2461 gttgttacag cacggtacgt tctcgcagga gtttcactcg ccggtcaatg tggatgagct
     2521 gatgtccgcc attctgtctg tgcactgatg aattttaccg aggaacctga agatgtctgc
     2581 ttcgtcatta tcattgccgc tgggcaagag cgtctcgctc aaacaatttg tcagtcgcca
     2641 tattaatgag atcggtttgc tggtggtaat cgccattctt tatctggtct tctccctgaa
     2701 tgcgcctggc tttatctcat tgaataacca gatgaacgtg cttcgcgatg ctgccaccat
     2761 cgggatcgcc gcctgggcaa tgacgctgat tattatctcc ggtgaaattg atgtcagcgt
     2821 tggaccgatg gtggcttttg tctcggtgtg cctggcattt ttgctgcaat ttgaagttcc
     2881 gttggcgatt gcttgtctgc tggtgttgct gttaggcgcg ctgatgggaa cgctcgccgg
     2941 ggtgctgcgc ggcgtgttta acgtgcccag tttcgttgcc acactggggc tgtggagcgc
     3001 cctgcgcgga atggggctgt ttatgaccaa cgcactgcca gtgccgattg acgaaaacga
     3061 ggtgctggac tggctgggcg gacaatttct cggtgtgcca gtatccgcgc tgatcatgat
     3121 ggtgttgttt gcgctgtttg tgttcattag ccgaaaaacc gccttcgggc gctcggtttt
     3181 tgccgttggc ggtaatgcca cggcggcgca gttgtgcggt attaacgttc gtcgagtgcg
     3241 cattcttatc tttacccttt cgggattatt agcggcggtg accggcattt tgctggcggc
     3301 acgcctcggt tccggtaacg caggtgccgc aaacggtctg gagtttgacg tcatcgccgc
     3361 ggtcgtggtc ggcggtacgg cactttccgg cggtcgcggt tccctgttcg gtacattgct
     3421 tggtgtgctg gtgattacgc taatcggtaa cggtctggtg ctgctcggta ttaactcctt
     3481 tttccagcag gtggtacgcg gcgtcatcat cgtggtggcg gtgctggcga atatcttgct
     3541 gacccagcga agcagtaaag cgaaacgcta aaccttaacg cccctggcgc gggtgcggca
     3601 gggatgaaaa ttaatcgtat tgttaatgag ggatttatga aaacgatgct ggcagcttat
     3661 ttaccaggaa attcaaccgt cgatctgcgg gaagttgcgg tgccgacgcc ggggattaac
     3721 caggtactga tcaaaatgaa atcctccggg atttgcggaa gcgatgtcca ctatatctat
     3781 catcaacacc gtgccacggc ggcggcaccc gataaaccgt tataccaggg atttatcaac
     3841 ggtcatgaac catgcgggca gattgtggcg atggggcaag gctgtcgcca ttttaaagag
     3901 ggtgatcgcg tgctggtgta tcacatttct ggctgtggtt tttgcccgaa ctgccgccgc
     3961 ggctttccta tctcttgtac tggcaaagga aaagcggctt acggctggca gcgtgacggc
     4021 ggtcatgccg aatacttgct ggcggaagaa aaagatctga tcctcctgcc ggatgcgctt
     4081 agctacgaag atggtgcgtt tatcagttgc ggcgttggta cagcgtatga aggaattttg
     4141 cgcggcgaag tttccggcag tgataacgtg ctggtggtcg gtctggggcc agtcggcatg
     4201 atggcgatga tgctggcgaa aggtcgcggt gcaaaacgga tcatcggcgt tgatatgctg
     4261 ccggaacgtc tggcgatggc aaaacagtta ggggtgatgg atcacggcta tttagcaacc
     4321 accgaaggtc tgccgcagat tatcgccgaa ctcacccacg gtggcgcgga tgttgcgctc
     4381 gattgttctg gtaatgccgc aggtcgcttg ctggcactgc gatccaccgc tgattgggga
     4441 cgggtggttt acatcggaga aaccggaaaa gtggagttcg aggtcagcgc cgatctgatg
     4501 caccatcaac ggcggattat cggctcctgg gtgaccagtc tgttccatat ggaaaaatgc
     4561 gcccatgatc tgacggactg gaaactgtgg ccgcgtaacg ccattaccca tcgcttctcg
     4621 ctggaacagg ctggagatgc ctatgcgctg atggcgagcg gcaaatgcgg gaaagttgtg
     4681 attaacttcc cggattaagg agttagcgga tgaccatcta taccttatct cacggaccgc
     4741 taaagctgga tgtctctgac cagggcggcg taatcgaagg cttctggcgt gacacgacgc
     4801 cgcttttacg tcctggtaaa aaaagcggtg tggcaaccga tgcttcctgt tttccgctgg
     4861 tgccgtttgc aaaccgggtg agcggcaatc gttttgtctg gcaggggcgt gagtatcaac
     4921 tgcaaccgaa tgttgagtgg gatgcgcact atctacacgg cgatggctgg ctgggcgagt
     4981 ggcaatgtgt ctcgcacagc gatgatagct tgtgtctggt gtatgaacat cgcagcggtg
     5041 tctatcacta tcgggtaagt caggcgtttc atttaacggc ggatacgctg acggtgacgc
     5101 tctctgtcac caatcaaggg gcagagacgc tgccatttgg taccggctgg catccttatt
     5161 ttccgttgtc accacaaacg cggattcagg cgcaggcgag cggttactgg ctggagcggg
     5221 agcagtggct ggcgggtgag ttttgcgagc agctaccgca ggaactggat tttaaccagc
     5281 tcgcgccgtt gccgcgccag tgggtgaaca atggttttgc cggatggaat ggtcaggccc
     5341 gcatcgagca gccgcaggaa ggttatgcca tcatcatgga aacgacgcca cctgcaccgt
     5401 gttatttcat ctttgtttcc gaccctgcgt ttgataaagg atatgcgttt gatttcttct
     5461 gtctggaacc gatgagccat gcgccggatg atcatcatcg cccggaaggg ggtgatctca
     5521 ttgcgctagc accaggggaa tcaacaactt cagagatgtc gttgcgggta gagtggctgt
     5581 aatttattaa atgccggata aggcgttcac gccgcatccg gcatctttcc taacgccgat
     5641 cgagagaaat tgcccccggc ccggtaattg ccaatagcaa gaacgcgcca gcgatactga
     5701 cattcttcca gaaattaatc atatttggcc caaccgcatc gccggtcata tcccagtaat
     5761 ggtgaccaat caccgccgta cccagcgtgt agaaaataaa cagcaccgcc agcggacggg
     5821 tgaaaaagcc aagcacgatt aatatcgcgg cgggcacttc cataactacc gcaataatcg
     5881 ctgccagcat cggcattggc gcgcccaacg aggccatata ttggaccgta ccgtcaaagc
     5941 ccatcatttt gggaaaaccc aaaataatga aaattaagac cacggcgata cgggcaatta
     6001 ataacaaaac ggggcgggca gctccaaaat caaaataacg tagtgtgttc ataaccaatc
     6061 cctttgcaag ataatgtgat ttaaacgtaa tacacactct tgcacctcgc cacgccccag
     6121 gcgttgagat attcgctaat tatcctggtt acagtgattt aagcgcgatg ttctcatcta
     6181 tcagcagttt cgcatcaaac gttttgccgc tctggatcca tttgcgaatt gggcgaatct
     6241 cacgcccctg attcagcgtg accgcaccga taagcacgcc gttttgcaga ttaaaccaaa
     6301 tcgccttctg agtttccggg ttgccacgac aaagccag
//
LOCUS       DAJVUJ010000200         6335 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.200, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000200 DAJVUJ010000000
VERSION     DAJVUJ010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6335
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.200"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(1..393)
                     /locus_tag="OQQ48_003812"
                     /pseudo
     CDS             complement(1..393)
                     /locus_tag="OQQ48_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151035092.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            complement(579..3887)
                     /gene="icsA"
                     /locus_tag="OQQ48_003813"
                     /gene_synonym="virG"
     CDS             complement(579..3887)
                     /gene="icsA"
                     /locus_tag="OQQ48_003813"
                     /gene_synonym="virG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter IcsA"
                     /protein_id="HCS1954404.1"
                     /translation="MNQIHKFFCNMTQCSQGGAGELPTVKEKTCKLSFSPFVVGASLL
                     LGGPIAFAIPLSGTQELHFSEDNYEKLLTPVDGLSPLGAGEDGMDAWYITSSNPSHAS
                     RTKLRINSDIMISAGHGGAGDNNDGNSCGGNGGDSITGSDLSIINQGMILGGSGGSGA
                     DHNGDGGEAVTGDNLFIINGEIISGGHGGDSYSDSDGGNGGDAVTGVNLPIINKGTIS
                     GGNGGNNYGEGDGGNGGDAITGSSLSVINKGTFAGGNGGAAYGYGYDGYGGNAITGDN
                     LSIINNGAILGGNGGHWGDAINGSNMTIANSGYIISGKEDDGTQNVAGNAIHITGGNN
                     SLILHEGSVITGDVQVNNSSILKIINNDYTGTTPTIEGDLCAGDCTTVSLSGNKFTVS
                     GDVSFGENSSLNLAGISSLEASGNMSFGNNVKVEAIINNWAQKDYKLLSADKGITGFS
                     VSNISIINPLLTTGAIDYTKSYISDQNKLIYGLSWNDTDGDSHGEFNLKENAELTVST
                     ILADNLSHHNINSWDGKSLTKSGEGTLILAEKNTYSGFTNINAGILKMGTVEAMTRTA
                     GVIVNKGATLNFSGMNQTVNTLLNSGTVLINNINAPFLPDPVIVTGNMTLEKNGHVIL
                     NNSSSNVGQTYVQKGNWHGKGGILSLGAVLGNDNSKTDRLEITGHASGITYVAVTNEG
                     GSGDKTLEGVQIISTDSSDKNAFIQKGRIVAGSYDYRLKQGTVSGLNTNKWYLTSQMD
                     NQESKQMSNQESTQMSSRRASSQLVSSLNLGEGSIHTWRPEAGSYIANLIAMNTMFSP
                     SLYDRHGSTIVDPTTGQLSETTMWIRTVGGHNEHNLADRQLKTTANRMVYQIGGDILK
                     TNFTDHDGLHVGIMGAYGYQDSKTHNKYTSYSSRGTVSGYTAGLYSSWFQDEKERTGL
                     YMDAWLQYGWFNNTVKGDGFTGEKYSSKGITGALEAGYIYPTIRWTTHNNIDNALYLN
                     PQVQITRHGVKANDYIEHNGTMVTSSGVNNIQAKLGLRTSLISHSCIDKETLRKFEPF
                     LEVNWKWSSKQYGVIMNGMSNHQIGNRNVIELKTGVGGRLADNLSIWGNVSQQLGNNS
                     YRDTQGILGVKYTF"
     gene            4416..5618
                     /gene="virA"
                     /locus_tag="OQQ48_003814"
     CDS             4416..5618
                     /gene="virA"
                     /locus_tag="OQQ48_003814"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HCS1954405.1"
                     /translation="MQTSNITNHERNDSSWMSTVKSTTEVSWNKLSFCDVLLKIITFG
                     IYSPHETLAEKYSEKKLMDSFSPSLSQDKMDGEFAHANIDGISIRLCLNKGICSVFYL
                     DGDKIQSTQLSSKEYNNLLSSLPPKQFNLGKVHTITAPVSGNFKTHKPAPEVIETAIN
                     CCTSIIPNDDYFPVKDTDFNSVWHDIYRDIRASDSNSTKIYFNNIEIPLKLIADLINE
                     LGINEFIDSKKELQMLSYNQLNKIINSNFPQQDLCFQTEKLLFTSLFQDPAFISALTS
                     AFWQSLHITSSSVEHIYAQIMSENIENRLNFMPEQRVINNCGHIIKINAVVPKNDTAI
                     SASGGRAYEVSSSILPSHITCNGVGINKIETSYLVHAGTLPSSEGLRNAIPPESRQVS
                     FAIISPDV"
     gene            5797..6335
                     /locus_tag="OQQ48_003815"
                     /pseudo
     CDS             5797..6335
                     /locus_tag="OQQ48_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     misc_feature    6077..6193
                     /locus_tag="OQQ48_003815"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT         1935 a         1440 c         1115 g         1845 t
ORIGIN      
        1 tcaggctgta cgcttcccca tgtgatttgc ctcgccactt caccgggctc aggaccccgt
       61 aggatcttca ccgaaaaaaa tgcatcacca gttgctttat tatttgtttt aggtagacct
      121 gttactaatt tatacctact ctgaccaagt ctggataaga aaggcacacc tgcacatttc
      181 tacttggaaa tgttttcttt taccattttg ctcaaaaatt tcaacatcca atattcctcg
      241 tacagaacta ctcaagtcct gaatagaaaa agttccagct ggtacagtag agtcatatat
      301 tgctcgacct tgttgagata caacaacacg tgcattagtt tctgcaatac cagtaatctg
      361 aggcgcataa ccacgaatat ttggcggtag cattcgttca tcgctttcta atgcaattcc
      421 tgtgtaacgc caagcaacag cgcattctgg cgcatgatct ggtaaacacg tttggcattg
      481 atcgcaggca taccatcatg tgcacaaaaa cccaccgagc ttttacacgg tgggtcccag
      541 agaaatgcag gacatcaaca cgccctgcat ttttattatc agaaggtata tttcacaccc
      601 aaaatacctt gggtgtctct gtaactgtta ttacccaatt gctgagatac gtttccccag
      661 atgcttaggt tatctgcaag acgccccccc acaccagttt tgagttcaat cacattacgg
      721 ttgcctatct ggtgatttga catgccattc ataattacac catattgctt tgagctccat
      781 ttccaattca cttccaaaaa tggttcgaac ttacgaagag tctccttatc gatacaacta
      841 tgactaatta aggatgtacg caatcccaat tttgcttgaa tattattgac cccagaggat
      901 gtgaccatag tgccattgtg ttcaatatag tcgtttgctt ttaccccatg cctagttatc
      961 tggacttgtg gattgagata caatgcgttg tcaatattat tatgagtagt ccagcgtatg
     1021 gttgggtaga tatagccagc ttccaaagct cctgttattc ctttgctgga atatttctca
     1081 ccagtaaacc catctccttt gactgtatta ttaaaccaac cgtactgcaa ccaagcatcc
     1141 atatatagac ctgttcgttc tttttcatcc tgaaaccaag aactgtacaa accggcagta
     1201 taaccgctca cagttcctcg tgaactataa ctagtatact tattatgagt tttgctatcc
     1261 tgatatccat aagctcccat aatacccaca tgcaagccat catgatcagt gaagtttgtc
     1321 ttcaaaatat ctccaccaat ctgataaacc atcctgttag ctgtggtttt taattgtcta
     1381 tcagctaaat tatgctcatt atgtccacca acagtacgaa tccacatggt ggtttcgctg
     1441 agctgacctg tagtaggatc aacaatagtg ctaccgtgtc ggtcatagag agaaggacta
     1501 aacatcgtgt tcattgctat caggttagca atataactgc cagcttcagg gcgccatgtg
     1561 tgaatactac cttcacccaa attaagtgaa gatacaagct gtgaactagc tcggcgacta
     1621 ctcatttgag tagactcttg attgctcatc tgttttgatt cttgattatc catctgacta
     1681 gttagatacc acttattggt attcagtcca gatacagtgc cctgtttcag gcgatagtca
     1741 taactaccag caacaatacg gcctttctga ataaaagcat tcttatcaga agaatctgtc
     1801 gaaataattt gaacaccttc aagagtttta tctccagagc ctccctcatt tgtcactgca
     1861 acataggtaa taccagacgc atggcctgta atttccagcc ggtcagtttt actgttgtca
     1921 ttgccgagaa ccgcgcccaa agataatatt ccgccctttc catgccaatt acctttctga
     1981 acataggtct gaccgacatt tgacgaacta ttattgagaa taacatgacc gtttttctcc
     2041 agagtcatgt tacctgtgac aatgacgggg tcaggcaaaa aaggggcatt aatattattg
     2101 attagcacag tcccactatt taataaagtg ttaacagttt ggttcatgcc tgaaaaattc
     2161 aaggtagcac ctttattaac aataacacca gcggtacgtg tcatagcttc aactgtcccc
     2221 atttttagaa tgcctgcatt gatgttggtg aaaccagagt aggtattttt ttccgccaaa
     2281 atgagagttc cctcccctga ttttgttagg gattttccgt cccagctatt tatattatga
     2341 tggctgagat tatctgccag aatagtacta acagtaagtt cagcgttttc tttcagattg
     2401 aactctccat gactgtcgcc atctgtatca ttccagctca aaccgtagat caatttattc
     2461 tggtcactga tatagctttt tgtatagtca atagcaccag tagtgagtaa cggattgatg
     2521 atagatatat tagaaacact gaaacctgtt atccctttat ctgcacttag cagtttatag
     2581 tccttctgcg cccagttatt tataatagct tccactttta cattgttgcc aaatgacata
     2641 tttccagaag cttccagact actgattcca gctaaattta aagaactgtt ctcaccaaaa
     2701 gaaacgtcac ctgaaacagt gaatttgtta cctgatagtg aaacagttgt acaatcacca
     2761 gcacataaat caccttcaat agttggtgtg gtcccagtgt aatcattgtt gataattttc
     2821 agaatggatg aattgttaac ctgtacatca ccagtaatga cagaaccttc atggagtatt
     2881 aatgaattgt ttccaccagt gatgtggata gcattacctg ctacattttg tgttccatca
     2941 tcttctttac ctgaaattat atatccgcta ttagcaatgg tcatattgct accatttata
     3001 gcatcccccc aatgtccacc attaccgcct aaaatagctc cattgttgat tatagacagg
     3061 ttatctcctg tgatagcatt accaccgtag ccatcataac cataaccgta agcagcacct
     3121 ccgttgcctc cagcgaacgt gcccttattg attacagaga ggctgcttcc tgtgatggca
     3181 tcacctccat taccgccatc accctcacca taattgttac ctccattacc accggaaata
     3241 gtccctttgt tgattatggg tagattgact cctgtgacgg catcacctcc attcccccca
     3301 tcactatcac tataactatc gccaccatgt ccacctgaaa taatttctcc atttattata
     3361 aacagattgt ctcctgtaac agcctcacca ccatcaccgt tatggtcagc accgctaccg
     3421 ccgctaccac caagaatcat gccttgattg attatagaca agtcagatcc ggtaatagag
     3481 tcaccaccat taccgccaca actattacca tcattattat caccagcacc accatgacct
     3541 gcgctaatca taatatcaga gttaatccgt agcttagttc tagatgcatg agaggggttg
     3601 gaagaagtta tataccacgc atccattcca tcttcaccag ctcccaaggg agaaagtcca
     3661 tcaacaggtg ttaataattt ttcataattg tcctctgaaa aatgaagttc ttgagtaccc
     3721 gaaagaggaa tagcaaaagc tattggcccc ccgagcaaca gggatgcacc aacaacaaaa
     3781 ggagaaaaag acaatttgca tgttttttcc tttaccgtag gtaattctcc ggcccccccc
     3841 tgtgaacatt gggtcatatt acaaaaaaat ttgtgaattt gattcatgca ctatattatc
     3901 agtaagtggt tgataaaccc ctaaaaagat tccgttttat ccggaataaa gggacgatat
     3961 atgcaaaaac atattaaaca aagcctcaac caaaacatga gagctgtgtt atatatcaat
     4021 gcaacgggag aaatttacct gtgtcttgct ttgaaaaaaa gagggggaaa gctatatgtt
     4081 cgataataaa ttaataattt tactatgata gtctgcttca gaccgcaact tttcagttct
     4141 ttctgtacgc ttgcccaaag tttaaccact ttgtttttct atacacccaa tggttctatc
     4201 tgccccttga tgttaatccc caaagccaat aacatgaatg tattcatatc acaactttcc
     4261 taccagactc tccaatgcaa taactgcata attgttttta gtggaaatgt atcgttttct
     4321 taaagagaag aataacattc cattttatta tgttcccaat ttaattatat taatatatat
     4381 tggcatcaat aggaaaatac atcaggagaa atcaaatgca gacatcaaac ataactaacc
     4441 atgaaagaaa tgactcatca tggatgtcaa ctgtcaaatc aactacagaa gtaagttgga
     4501 ataaattgag tttttgtgac gttttactga aaataataac tttcggaata tattctccac
     4561 acgaaactct agccgaaaaa tatagcgaaa aaaaactaat ggatagtttc tcaccatcac
     4621 tatcacaaga taaaatggac ggagagtttg cccacgcaaa catagatggc atttcaatac
     4681 ggctatgcct taacaaaggc atatgttcgg ttttttatct tgatggagat aaaatacaat
     4741 ccactcaact ttcatcaaaa gaatataata acttgttaag ttcactcccc cccaaacaat
     4801 tcaacttagg aaaagtacat actattacag ctccggtatc agggaatttt aaaactcaca
     4861 agcctgcacc agaagttatt gaaacagcca ttaattgctg tacatcgata ataccaaacg
     4921 atgattactt ccccgttaaa gacactgatt ttaatagtgt ttggcatgat atttacagag
     4981 atattcgagc atctgacagc aatagtacaa aaatatattt caacaatatt gaaataccac
     5041 taaaattaat tgcagatcta attaatgagt taggaattaa tgaattcatt gatagcaaaa
     5101 aagagcttca aatgctaagt tacaaccaat taaataaaat aatcaattca aacttccccc
     5161 aacaagacct atgttttcaa acagaaaaac ttttgtttac ttcactcttt caagaccctg
     5221 catttatatc cgcactaaca tctgcattct ggcaatctct tcacatcacg tcttcctctg
     5281 tggaacacat atatgcccaa attatgtccg aaaacataga aaacaggctt aatttcatgc
     5341 ctgaacaacg agttattaac aattgtggac atattataaa aatcaacgca gttgtcccta
     5401 aaaacgacac tgcaatctct gcctctgggg ggagggctta cgaagttagc tcatcaatat
     5461 taccatcgca cataacatgt aatggtgttg gtattaataa aatagaaaca agctatctcg
     5521 ttcatgctgg aacacttcca agcagtgaag ggcttcgcaa tgctatccct ccagaaagtc
     5581 gtcaagtatc atttgccatc atatctcctg atgtttaagc caatattata ttggctgcca
     5641 gactgtaaaa aaacgttctt cagaacagct ctataaacta ttaaaccaaa gctaaaagga
     5701 tggggaccaa tagaatctca gcccatcctt tttatatcat tatgaaccgc cccggaaatc
     5761 ctggagacta aactccctga gaaagaggta aacaggatga ctaaaaatac acgtttttcc
     5821 cctgaagtcc gtcaacgggc agttcgtatg gttctggaaa gtcagggcga atatgactca
     5881 caatgggcag cgatttgttc cattgctcca aagattggct gtatactgga gactctgcgt
     5941 gtctgggtac gccagcatga gcgggatacc gggggcggtg aggtgggctc accaccgctg
     6001 aacgtcagcg tctgaaagag ccggaacgtg aaaatcgtga actgcgccgc agtaacaata
     6061 tccttcgcct ggcttccgct tattttgcaa aggcggagtt cgaccgcctc tggaaaaaat
     6121 aatgccactg ctggataagc tgcgtgagca gtacggggtc ggaccggtat gcagtgaact
     6181 gcatattgcc ccgtcaacgt attaccactg tcagcaacag cgacatcatc atgataaacg
     6241 cagtgcccgt gcgcagcgcg atgactggct gaagaaagag atactgcgcg tatacgatga
     6301 gaatcatcag gtatacgctg tgcgtaaagt caggc
//
LOCUS       DAJVUJ010000201         6334 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.201, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000201 DAJVUJ010000000
VERSION     DAJVUJ010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6334
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.201"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..526
                     /locus_tag="OQQ48_003816"
     CDS             <1..526
                     /locus_tag="OQQ48_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCS1954406.1"
                     /translation="LPWLSEADRRLQVQSDLPWWLVCRGAIHKFRCVPHLTGRRFEHG
                     VTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEATGLYQVPLSSAQPGDV
                     LLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRRTHSLWRHREWHASAF
                     TGICNDLAAASTFV"
     gene            463..1065
                     /locus_tag="OQQ48_003817"
     CDS             463..1065
                     /locus_tag="OQQ48_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCS1954407.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLVGAKSGGVFQAVLGAAVMAVA
                     IWMPGVGIMASNLLFSLGASMTLGGVAQMLAPKARTPRTQTTDNGKQNTCFSSLDNMV
                     AQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            1126..4605
                     /locus_tag="OQQ48_003818"
     CDS             1126..4605
                     /locus_tag="OQQ48_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCS1954408.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVDGPVDGLKS
                     VLLNGTPVLDSEGKTNFSGVTVVFRAGEQEQTPPEGVESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRLTFGVQALVENTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGNQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCR
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNVVEVNWIDPDNGHETATELVEDAQAILRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSCTTLISLVDGNGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDRRGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFRQ
                     LALGNYSLTVRAVNARGQQGDPASVSFRIAAPAAPVTIELIPGYFQITAVPKLAVYDP
                     TVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIRPGHDYYFYVRSVNTVGKS
                     AFVEAVGRASDDAEGYLSFYKGLINNTHLGKELWTQIDNGQLAPDLTEIRTSITNVSN
                     EITQTVNKKLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGAGIEN
                     TPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNP
                     PAFSLTPGGRLTAKNADISGNVNANSGTLNNVTINKNCRVLGKLSANQIEGDLVKTVG
                     KPFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFRGAKHERKNNNIYSSCSLI
                     VKKNGAEIYNRTTLDNTLIYTGVIDMPAGHGHMTLEFSVSAWLVNGWYPTASISDLLV
                     VVMKKATAGITIS"
     gene            4673..5272
                     /locus_tag="OQQ48_003819"
     CDS             4673..5272
                     /locus_tag="OQQ48_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCS1954409.1"
                     /translation="MRKVCAAILSAAICLAVSGAPAWASEHQSTLSAGYLQPHTDMPG
                     SDDLKGINVKYRYEFTDTLGLVTSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDNRHSNTSLAW
                     GAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGVGYKF"
     gene            5336..>6334
                     /locus_tag="OQQ48_003820"
     CDS             5336..>6334
                     /locus_tag="OQQ48_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCS1954410.1"
                     /translation="MAVKISGVLKDGAGKPVVNCAIELRARRTSPTVVAHVVATCVTD
                     NNGAYVIEAEPGYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNAFLNAPKDGDLRPEV
                     MKRFEEMVVQAQQSAGAAAGNAQQTAQDVAAAATARDDAQRFAENARQDATVTAEDRK
                     ATAEDVTSTGANAAAAGQSAQDAAGYARAAEQAKNDIDAALTGTLKMANHLSEIAAAG
                     EKAQQKSRDNLGLKSAATMEAQSDIYDRTKGRLAIPGAFGFGCAFLPEDVIRFDTKSD
                     FLAWVRNALPGEYSVAGPYDIIIPDTRFEGVLSIRWTDARPETTEPRYRAKSLTFYG"
BASE COUNT         1457 a         1559 c         1993 g         1325 t
ORIGIN      
        1 tctgccctgg ctgagtgagg ctgaccggcg gctgcaggtg cagagtgatt tgccgtggtg
       61 gctggtctgc cggggggcga ttcacaagtt ccgctgtgtg ccgcatctca ccgggcggcg
      121 ctttgagcac ggggtgacgg actgttacac gctgttccgg gatgcttatc atctggcggg
      181 gattgagatg ccggattttc atcgcgggga tgactggtgg cgtcacggtc agaatctcta
      241 tctggataat ctggaggcca cagggctgta tcaggtgccg ttgtcatcag cacaaccggg
      301 cgatgtgctg ctgtgctgtt ttggttcatc ggtgccgaat catgccgcca tttactgcgg
      361 cgacggtgag ctgctgcacc atattcctga acaactgagt aaacgagaga ggtataccga
      421 caaatggcag cgacgcacac actccctctg gcgtcaccgg gaatggcacg catctgcctt
      481 tacggggatt tgcaacgatt tggccgccgc atcgaccttc gtgtgaaaac gggggctgaa
      541 gccatccgcg cactggccac acagctcccg gcgtttcgtc agaaactgag tgacggctgg
      601 tatcaggtgc gcattgccgg gcgtgatgca ggtgaaaccg aattgtctgc ccgtcttaat
      661 gagccgctgg caaatggtgc agtgatccac atagtaccgc gtctggtggg agctaaaagt
      721 ggcggtgtgt ttcaggcggt gctgggggca gctgttatgg cggttgctat atggatgccg
      781 ggggtaggaa ttatggcgag taatctgctg ttttctctcg gtgccagtat gacgcttggc
      841 ggtgttgcac agatgctggc accgaaagcc agaactcccc gtacacagac aacggataac
      901 ggcaaacaga acacctgttt ctcctcactg gataacatgg ttgcccaggg caatgttctg
      961 cctgttctgt acggtgaaat gcgcgtgggg tcgcgggtgg tatctcagga gatcagcacg
     1021 gcagacgaag gggatggtgg tcaggttgtg gtgattggtc gctgatgcaa aatgtttcat
     1081 gtgaaaccgc ctgcgggcgg ttttgtcgtt tatggagcgt gaggaatggg taaaggcagc
     1141 agtaaggggc ataccccgcg cgaagcgaag gacaacctga aatccacgca gttgctgagt
     1201 gtgattgatg ccatcagcga agggccggtt gacggtccgg tggatggatt aaaaagcgtg
     1261 ctgctgaacg gtacgccagt cctggacagc gaggggaaga caaacttttc cggtgttacg
     1321 gtggtgttcc gcgccggcga gcaggagcag acaccgccgg agggggttga atcttccggc
     1381 tcagagactg tgctgggtac ggaagtgaaa tacgacacgc cgatcacccg gaccatcacg
     1441 tcggcaaaca ttgatcgtct gcgcctgacc ttcggtgtgc aggcactggt ggaaaacacc
     1501 tcaaaggggg accggaatcc gtcggaagtc cgcctgctgg ttcagataca gcgtaacggt
     1561 ggctgggtga cggaaaaaga catcaccatt aagggcaaaa ccacttcgca gtatctggcc
     1621 tcggtggtgg tgggtaacct gccgccgcgc ccgtttaata tccggatgcg caggatgaca
     1681 ccggacagca ccacagacca gctgcagaac aaaacgctct ggtcgtcgta caccgaaatc
     1741 atcgatgtga aacagtgcta cccgaacacg gcgctggtcg gcgtgcaggt ggattcagag
     1801 cagttcggta accagcaggt gagtcgcaat tatcatcttc gcgggcgcat tctgcaggtg
     1861 ccgtcgaact ataacccgca gacgcggcaa tacagcggta tctgggacgg aacgcttaag
     1921 ccagcataca gcaacaacat ggcctggtgt cggtgggaca tgctcactca tccgcgctac
     1981 ggcatgggga aacgtcttgg tgcggcagat gtggacaaat gggcgctgta tgtcatcggc
     2041 cagtactgcg accagtcagt gccggacgga tttggcggca cggagccgcg catcacctgt
     2101 aacgcttacc tgaccacaca gcgtaaggcg tgggatgttc tcagtgattt ctgctcggcg
     2161 atgcgctgta tgccggtatg gaacgggcag acgctgacgt tcgtgcagga ccgaccgtcg
     2221 gataaagtgt ggacctataa ccgcagtaat gtggtgatgc cggatgatgg cgcgccgttc
     2281 cgctacagct tcagcgccct gaaggaccgc cataatgtcg ttgaggtgaa ctggattgat
     2341 ccggacaacg gtcatgagac ggcgacagag cttgtggagg acgcgcaggc cattctccgt
     2401 tacggtcgta acgtcacgaa gatggatgcc tttggctgta ccagccgggg acaggcgcac
     2461 cgcgccgggc tgtggctgat taaaacggaa ctgctggaaa cgcagaccgt ggacttcagc
     2521 gtcggcgcag aagggcttcg ccatgtaccg ggtgatgtca ttgaaatctg cgatgatgat
     2581 tatgccggta tcagcaccgg tggtcgcgta ctggcggtga acagccagac ccggacgctg
     2641 acgctcgacc gtgaaatcac gctgccgtcc tcctgtacca cgctgataag cctggttgac
     2701 ggaaatggca atccggtcag cgtggaggtt cagtccgtca ccgacggcgt gaaggtgaaa
     2761 gtgagccggg ttcctgacgg cgttgcagaa tacagcgtgt gggggctgaa gctgccgacg
     2821 ctgcgccagc gcctgttccg ctgcgtgagt atccgtgaga acgacgacgg cacgtatgcc
     2881 atcaccgccg tgcagcatgt gccggaaaaa gaggccatcg tggataacgg ggcacacttt
     2941 gacggtgacc ggcgcggcac ggtgaatggt gtcacgccgc cagcggtgca gcatctgacc
     3001 gccgaagtca ccgcagacag cggggaatat caggtgctgg cgcgatggga cacgccgaag
     3061 gtggtgaagg gtgtgagctt cctgcttcgc ctgaccgtgg cagcggacga cggcagtgag
     3121 cggctggtca gcacagccag gacgacggaa accacatacc gcttcaggca gctggcgctg
     3181 gggaattaca gtctgacagt ccgggcggta aatgcccggg ggcagcaggg cgatccggcg
     3241 tcggtatcgt tccggattgc ggcaccggca gcgcctgtca ctattgaact gataccgggg
     3301 tattttcaga taacggcggt cccgaaactg gctgtatacg acccgacggt gcagtttgag
     3361 ttctggtttt cggaaaagcg gattgcggat atcaggcagg ttgaaaccag cgcccgctat
     3421 cttggcacgg cgctgtactg gatagccgcc agtatcaata tcaggccggg ccatgattat
     3481 tatttttacg ttcgcagtgt gaacaccgtt ggcaaatcgg catttgtgga ggccgtcggt
     3541 cgggcgagcg atgatgcgga aggttatctg agtttttata aagggttgat caataacacg
     3601 catctcggca aggagttgtg gacgcagatt gataacggtc agcttgcgcc ggacctgact
     3661 gaaatcagga cgtccattac gaatgtcagc aatgaaatca cgcaaaccgt caataaaaaa
     3721 ctggaaaatc agagtgctgc aatccagcag atacagaaag ttcaggttga tacaaataat
     3781 aacctgaaca gcatgtgggc tgtgaagctg caacagatga aggacggacg cctttatatt
     3841 gcgggtatcg gtgccggtat tgagaatacg ccagcaggaa tgcagagtca ggtgctgctg
     3901 gcggcagaca ggattgcgat gattaatcct gcgaatggca acacaaagcc gatgtttgtt
     3961 ggtcagggcg atcagatatt catgaacgac gtgttcctga aacgcctgac ggctccgacc
     4021 attaccagcg gcggtaatcc tccggcattt tccctgacac ctggcggacg gctgacggcg
     4081 aaaaatgccg atatcagcgg taacgtgaac gcgaactccg ggacgctcaa caacgtcacg
     4141 attaataaga actgtcgggt tctgggaaaa ttgtccgcga accagattga aggcgatctc
     4201 gttaaaacag tgggcaaacc tttcccacgg gactcccggg caccggagag gtggccatca
     4261 gggaccatta ccgtcagggt ttatgacgat cagccgtttg atcggcaaat tgttattccc
     4321 gcggtggcgt ttcgcggtgc taaacatgag cggaagaata acaatattta ttcgtcatgc
     4381 agcctgatag tgaagaaaaa cggtgctgaa atttataacc gaacgaccct ggataatacg
     4441 ctgatatata cgggtgttat tgatatgcct gccggtcacg gtcacatgac gctggagttt
     4501 tctgtatcgg catggctggt aaatggctgg tatcccacag caagtatcag cgatttgctg
     4561 gttgttgtga tgaagaaagc cactgcaggc atcacgatta gctgaatttt ataacccaga
     4621 tacgggcacc agaaatggtg ccttttttat tgcagaaaag cgagaggtaa ttatgcgtaa
     4681 agtttgtgca gccattttgt ccgcagccat ctgtctggct gtatccggtg cgcctgcatg
     4741 ggcgtctgaa catcagtcca cgctgagcgc cgggtatctt cagccccata ctgatatgcc
     4801 aggcagcgat gacctgaagg gcattaacgt gaaataccgt tatgagttta cggacacgtt
     4861 ggggctggtg acgtcattca gctatgccaa cgctgaagat gagcaaaaaa cgcattacag
     4921 cgatacccgc tggcatgagg attccgtgcg taaccgctgg ttcagcgtga tggcggggcc
     4981 gtctgtgcgc gtgaatgaat ggttcagcgc gtatgcgatg gcgggcgtgg cttacagccg
     5041 tgtgtcgacc ttctccgggg attatctccg cgtaactgac aacaagggga aaacgcacga
     5101 tgtgctgacc gggagtgatg acaaccgcca cagcaacacg tctctggcgt ggggggctgg
     5161 cgtgcagttt aacccgaccg aatccgtggc cattgacctt gcttatgaag gttccggcag
     5221 tggcgactgg cgcactgacg gtttcatcgt gggtgtcggt tataagttct gattagccag
     5281 gtaacacagt gttatgacag cccgctggtt caggcgggct ttttgtgggg tgaatatggc
     5341 agtaaagatt tcaggtgtac tgaaagatgg ggcgggtaaa cctgtcgtaa attgtgcgat
     5401 tgaactgcgg gccagaagaa ccagtccgac cgttgtggca cacgttgttg ccacttgcgt
     5461 gacggacaat aacggtgctt atgtgattga ggctgagccg gggtattacg aggttgcgct
     5521 tcactgtaac ggctggcagc caacccgtgt cggggatatt gatgtggcac cgactgatgc
     5581 accggggaca ctgaacgcgt ttctgaatgc accaaaggat ggtgatttac gtccggaggt
     5641 gatgaagcgc tttgaggaaa tggtggtgca ggcgcagcag agcgccgggg ccgcagccgg
     5701 aaacgcacag cagacggcgc aggatgtggc ggcagccgca acggcccgtg atgatgcaca
     5761 acgttttgcg gagaatgcca gacaggatgc aaccgtcaca gctgaggaca gaaaggccac
     5821 tgcggaagat gtgacaagca caggagcaaa tgcagccgca gccggacaga gcgcacagga
     5881 tgccgcaggt tatgcccgcg cagcagaaca ggccaaaaat gacattgatg ctgcgctgac
     5941 cggcactctg aaaatggcta accatctgtc agaaatcgca gcagcaggcg aaaaggcaca
     6001 acagaagtcc cgggataatc tggggctgaa aagtgcggcc acgatggaag cacagagcga
     6061 catttacgac cggacaaaag gccgtctggc gatacccggc gcattcggct ttgggtgtgc
     6121 ttttctgcct gaagatgtta tccgttttga cactaagagt gatttcctgg cctgggtaag
     6181 gaatgcgctg ccaggtgaat attccgttgc tggcccctac gacatcatca tacccgacac
     6241 acggtttgaa ggggtgctca gcatccggtg gactgatgca cgccctgaga caacagaacc
     6301 gcggtacaga gccaaatccc ttacttttta cggc
//
LOCUS       DAJVUJ010000202         6328 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.202, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000202 DAJVUJ010000000
VERSION     DAJVUJ010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6328
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.202"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..1194
                     /gene="tnaB"
                     /locus_tag="OQQ48_003821"
     CDS             <1..1194
                     /gene="tnaB"
                     /locus_tag="OQQ48_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HCS1954411.1"
                     /translation="AGTVIGGGMFALPVDLAGAWFFWGAFILIIAWFSMLHSGLLLLE
                     ANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTYAYISANGAIISETISMNL
                     GYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKIISFVIVFGSFFFQVDYSI
                     LRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIICYGKRKDKLIKSVVFGSLL
                     ALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLGTKQHGIIEFCLLVFSNLA
                     VASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPPALLYLIFPNGFIYGIGGA
                     GLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIVILFGITVILCWFGNVFNV
                     LPKFG"
     gene            1326..2501
                     /gene="mdtL"
                     /locus_tag="OQQ48_003822"
     CDS             1326..2501
                     /gene="mdtL"
                     /locus_tag="OQQ48_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HCS1954412.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            2476..3435
                     /gene="yidZ"
                     /locus_tag="OQQ48_003823"
     CDS             2476..3435
                     /gene="yidZ"
                     /locus_tag="OQQ48_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HCS1954413.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            3592..4341
                     /locus_tag="OQQ48_003824"
     CDS             3592..4341
                     /locus_tag="OQQ48_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HCS1954414.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            4363..4929
                     /gene="yieF"
                     /locus_tag="OQQ48_003825"
                     /gene_synonym="chrR"
     CDS             4363..4929
                     /gene="yieF"
                     /locus_tag="OQQ48_003825"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HCS1954415.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDRQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(4983..6320)
                     /gene="adeP"
                     /locus_tag="OQQ48_003826"
     CDS             complement(4983..6320)
                     /gene="adeP"
                     /locus_tag="OQQ48_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HCS1954416.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANSETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
BASE COUNT         1487 a         1633 c         1593 g         1615 t
ORIGIN      
        1 gcaggtacag taattggtgg aggtatgttt gctttacctg ttgatcttgc cggtgcctgg
       61 tttttctggg gtgcctttat ccttatcatt gcctggtttt caatgcttca ttccgggtta
      121 ttgttattag aagcaaattt aaattatcct gtcggctcca gttttaacac catcaccaaa
      181 gatttaatcg gtaacacctg gaacattatc agcggtatta ccgttgcctt cgttctctat
      241 atcctcactt atgcctatat ctctgctaat ggtgcgatca ttagtgaaac gatatcaatg
      301 aatttgggct atcacgctaa tccacgtatt gtcgggatct gcacagccat tttcgttgcc
      361 agcgtattgt ggataagttc gttagccgcc agtcgcatta cctcattgtt cctcgggctg
      421 aagattatct cctttgtgat cgtgtttggt tctttcttct tccaggtcga ttactccatt
      481 ctgcgcgatg ccaccagcac cactgcggga acgtcttact tcccgtatat ctttatggct
      541 ttgccggtgt gtctggcgtc atttggtttc cacggcaata ttcccagcct gattatttgc
      601 tatggaaaac gcaaagataa gttaatcaaa agcgtggtat ttggttcgct gctggcgctg
      661 gtgatttatc tcttctggct ctattgcacg atggggaata ttccgcgcga aagctttaag
      721 gcgataatct cctcaggcgg caacgttgat tcgctggtga aatcgttcct cggcaccaaa
      781 cagcacggca ttatcgagtt ttgcctgctg gtgttctcta acttagctgt tgccagttcg
      841 ttctttggtg tcacgctggg gttgttcgat tatctggcgg acctgtttaa gattgataac
      901 tcccacggcg ggcgtttcaa aaccgtgctg ttaaccttcc tgccacctgc gttgttgtat
      961 ctgatcttcc cgaacggctt tatttacggg atcggcggtg ccgggctgtg cgccaccatc
     1021 tgggcggtca ttattcccgc agtgcttgca atcaaagctc gcaagaagtt tcccaatcag
     1081 atgttcacgg tctggggcgg caatcttatt ccggcgattg tcattctctt tggtataacc
     1141 gtgattttgt gctggttcgg caacgtcttt aacgtgttac ctaaatttgg ctaaatcctt
     1201 caagaagcca gccattcgct ggcttcttgc ctctcaggaa atcacttatg tccaaatggc
     1261 aactcgcctg atcctccttc accacgtatg ctttgcgtca ccttactatc aggacgcttt
     1321 agcccatgtc ccgctttttg atttgtagtt ttgccctggt tttactttat cccgccggga
     1381 ttgatatgta cctcgttggt ttaccgcgca tcgccgccga tctcaatgcc agcgaagcgc
     1441 agttgcatat tgcgttctcc gtatatctgg cggggatggc agctgcgatg ttatttgccg
     1501 gtaaagtggc cgatcgttca gggagaaagc cggtcgccat acccggcgcg gcgctattta
     1561 ttattgcctc ggtgttctgt tcactggctg aaaccagcac gttatttctt gcaggccgat
     1621 ttctacaggg gttgggcgca ggctgttgtt acgtagtggc gttcgccatt ttgcgcgaca
     1681 cgctggatga tcgacgtcgg gctaaagtgc tgtcattact caacggtatt acctgcatca
     1741 ttccggtgtt agcgccagtg ctcggacatc tgattatgct taaattcccg tggcagagtc
     1801 tgttctgggc gatggcaatg atgggcatcg cggtactgat gttgtctttg tttattttaa
     1861 aagaaacgcg cccagcgtcc cccgctgctt cggacaaacc acgagaaaat agcgagtcgc
     1921 tgcttaatcg gtttttcctc agccgtgttg ttatcaccac cctcagcgtt tcggtgatcc
     1981 tcactttcgt caatacatcg ccggtattgc tgatggaaat catgggtttt gagcgcggag
     2041 aatacgccac cattatggcg ttgaccgctg gcgtcagcat gaccgtttca ttctccacgc
     2101 catttgcgct gggaattttt aagccacgta cgttgatgat cacctcgcag gtgttattcc
     2161 ttgcagcggg gatcaccctt gccgtttcac cttcccatgc ggtttctctg tttggtatca
     2221 cactgatttg cgccggtttc tcggtaggtt ttggcgtagc gatgagtcag gcgttaggac
     2281 cattttcatt acgcgcgggc gtagccagct cgaccttagg tattgcgcag gtttgcggtt
     2341 cgtcactgtg gatttggctg gcagcggtgg ttggtatcgg cgcatggaat atgctgatcg
     2401 ggattctgat tgcctgtagc atagtgagcc tgttgctgat tatgttcgtc gcgcctggac
     2461 gccccgttgc cgctcatgaa gaaatccatc accacgcttg atctcaatct gctgctctgt
     2521 ctgcaactgc tgatgcagga gcgcagcgtg accaaagcgg cgaagcggat gaacgtgacc
     2581 ccttcagcgg tgagtaagtc gctggcaaag ttaagagcgt ggtttgacga cccgctcttt
     2641 gtgaactcac cgctggggct gtcgcccaca ccgctaatgg tcagcatgga gcaaaatctg
     2701 gcggaatgga tgcaaatgag caatctgctg ctggataaac cgcaccacca gacaccgcgc
     2761 ggcctgaagt ttgagttggc ggcggaatcg ccgctgatga tgatcatgct taatgcgctg
     2821 tcgaaacgga tctaccaacg ttacccgcag gcgaccatca aactacgtaa ctgggattac
     2881 gattccttag atgccattac tcgtggtgaa gtggatattg gtttttccgg tcgcgaaagc
     2941 catcctcgct cgcgggagct gttaagctcg ctaccgttag ccattgatta tgaagtgctg
     3001 tttagtgatg taccctgcgt ctggttacgc aaagatcatc cggcactgca tgaaacgtgg
     3061 aatctggaca cctttttacg ttatccgcat atcagcattt gctgggaaca gagcgacacc
     3121 tgggcgctgg acaatgtgtt acaggagctg gggcgcgaac gcacgattgc tatgagcctg
     3181 ccggaatttg agcagtcact gtttatggca gcgcaacccg acaatctgct actggcgacc
     3241 gcgccgcgct actgtcagta ctacaatcaa ctccatcaac tgccgttggt tgctcttcct
     3301 ctcccgtttg acgaaagcca gcaaaaaaag ctggaagttc cttttaccct gctgtggcat
     3361 aaacggaaca gtcataatcc gaagatcgtc tggttacggg aaaccattaa aaacctttac
     3421 gcgtcgatgg cataaccgaa tcgtatgaaa cgcgacccaa tttcacaata aaatgtaaaa
     3481 aagttgtaat aagcttgtct gaatcgaact tttagccgct ttagtctgtc catcattcca
     3541 gtaaatgatt actcttatat tcataatgga ccattaagca tggagcgaaa aatggcgact
     3601 cactttgccc gagggatttt aacggaagga catctgattt ctgttcgtct cccctcccag
     3661 tgtcatcaag aagcccgaaa cattccccct catcgtcaaa gccgttttct ggcgtccaga
     3721 ggtttactcg cagaactgat gttcatgctg tatggcattg gcgaattgcc ggaaatcgtc
     3781 accctgccga aaggtaaacc ggttttcagt gataaaaatt tgccttcgtt ttctatttcc
     3841 tatgccggga atatggttgg cgtggcgtta acaaccgaag gtgaatgtgg cctcgatatg
     3901 gaactacagc gtgcgacgcg cgggtttcat agcccacacg cgcccgataa ccacaccttt
     3961 tccagcaatg aatcgctatg gatcagtaaa caaaacgatc ctaacgaagc gcgggcgcag
     4021 ctcatcacgc tgcgccgaag cgtgctaaaa ctaaccggtg atgttttgaa tgacgatccg
     4081 cgcgatctgc agctgctgcc cattgccgga cgcctgaaat gtgctcatgt aaatcatgta
     4141 gaagcgttat gcgacgcgga ggacgtgctg gtgtggtccg tggcggtcac gcccgcgatt
     4201 gaaaagctca gtgtctggga gttagatggc aaacacagct ggaaaagcct gccggatatt
     4261 cacagccgcg ccaacaatcc taccagccgg atgatgcgtt ttgcccagct ctctaccgtg
     4321 aagtcttttt cgccaaattg atagacaacc acaggagtca ttatgtctga aaaattgcag
     4381 gtggttacgt tactggggag cctgcgcaaa ggctcattta atggcatggt tgcacgtacc
     4441 ctgccgaaaa ttgctccggc gagcatggaa gtcaatgcgt taccttccat tgccgacatt
     4501 cccctgtatg acgctgacgt acagcaggaa gaaggttttc cagcaacggt tgaagctctg
     4561 gcggaacaga tccgtcaggc tgacggtgtg gtgatcgtca cgccggaata taactactcg
     4621 gtaccgggtg ggctgaaaaa tgccatcgac tggctttccc gcctgccgga tcaaccgctg
     4681 gccggtaaac cggtgttgat tcagaccagc tcaatgggcg tgattggcgg cgcgcgctgt
     4741 cagtatcacc tgcgccagat tctggttttc ctcgatgcaa tggtgatgaa caagccggaa
     4801 tttatgggcg gcgtgattca gaacaaagtt gatcggcaaa ccggagaagt gattgatcag
     4861 ggtacgctgg accacctgac cgggcaattg accgcatttg gtgagtttat tcagcgagtt
     4921 aagatctaaa taaaaaaccc gccagtaatc atgcatggcg ggtttttaat gcgctatcga
     4981 ttttaatgag cgtcgataaa tacaatcttc aggataaaca gcagcgcaac gatgattacg
     5041 cacgggctaa ggtcacgcag acgcccggta ccaatcttca tcacgcagta ggagataaag
     5101 cccagcgcaa taccttcggt aatcgaaaag ctgaacggca tcatcacggc ggtaataaac
     5161 gccggaacag attcagtaag atcctgccag ttcacgcgag caagacttga ggtcatcaac
     5221 acgccaacgt agatcaacgc gccagctgca gcgtagcctg gcaccattcc cgccagcggc
     5281 gacagaaaga taaccagcag gaacagcaga ccaacaacca ctgccgtcag accggtacga
     5341 ccgccaaccg atacgccgga agaggactca atataagccg taacggaaga agtaccgata
     5401 aacgaaccgg tcacggaaga gatactgtcg acatacagcg cttgcttcat gcgcgggaat
     5461 ttccccttct catccgccag acctgcttta tcggtcacgc caatcagcgt accggaggag
     5521 tcaaacaagt tgaccaacat gaaagagaaa atcacccctg ccagcccgag gttaaacgac
     5581 ccggctaaat ctacatgacc cacaactgtc attacgctcg gcggcgcaga aacgatgcca
     5641 ttgtagtgca catcacccag catccagccc agcagcgtcg tcaccacgat agaaaccagc
     5701 accgctgcgt gaatgttgcg cgaggccaga atagcaatga tgaagaagcc gaggataccc
     5761 agaagtacgc tgtgagaagt cagattaccg atgctcacca gcgtttccga gttagcgaca
     5821 atcacacctg cgtttttcag ccccatcatg ccgataaaca ggccgatacc gctggtaata
     5881 cccacacgca gacttaccgg aatgttagca atcatccagt aacgaacgcg gaaaatggtc
     5941 aaaagaagca gtccgacagc gccccagaag attgcgccca taccgatctg ccacggcagc
     6001 cccattgcct ggacaaccac aaaagcgaaa aaggcgttaa gccccatcgc aggagccagc
     6061 gccaccggaa ggtttgcaaa caatcccatc ataatgctac cgaaagcagc aatcagacag
     6121 gttgttacga agacggcact ggtgtccatg ccagcaacgc ccagaatttg tgggttaaca
     6181 aaaacgatgt aaaccatcgt caggaaggtg gtaaaaccgg cgatcacttc ggtccgtgcc
     6241 gtcgtgccat gttcgcgcag tttaaacacg cgttccagca tcccctggcc agaagcctgg
     6301 gttgtgtgtt gttgactcat tatctatt
//
LOCUS       DAJVUJ010000203         6191 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.203, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000203 DAJVUJ010000000
VERSION     DAJVUJ010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6191
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.203"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            158..499
                     /gene="raiA"
                     /locus_tag="OQQ48_003827"
     CDS             158..499
                     /gene="raiA"
                     /locus_tag="OQQ48_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCS1954417.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    602..727
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            603..650
                     /gene="pheL"
                     /locus_tag="OQQ48_003828"
     CDS             603..650
                     /gene="pheL"
                     /locus_tag="OQQ48_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HCS1954418.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            750..1910
                     /gene="pheA"
                     /locus_tag="OQQ48_003829"
     CDS             750..1910
                     /gene="pheA"
                     /locus_tag="OQQ48_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HCS1954419.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQQQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(1953..3074)
                     /gene="tyrA"
                     /locus_tag="OQQ48_003830"
     CDS             complement(1953..3074)
                     /gene="tyrA"
                     /locus_tag="OQQ48_003830"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HCS1954420.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLKQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3085..4155)
                     /gene="aroF"
                     /locus_tag="OQQ48_003831"
     CDS             complement(3085..4155)
                     /gene="aroF"
                     /locus_tag="OQQ48_003831"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HCS1954421.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDLETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4363..4728
                     /locus_tag="OQQ48_003832"
     CDS             4363..4728
                     /locus_tag="OQQ48_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HCS1954422.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFGDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVESASQ
                     LHEVWQKGADENASTIRLN"
     gene            4878..5396
                     /locus_tag="OQQ48_003833"
     CDS             4878..5396
                     /locus_tag="OQQ48_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HCS1954423.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAKQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            5386..>6191
                     /gene="dgcN"
                     /locus_tag="OQQ48_003834"
     CDS             5386..>6191
                     /gene="dgcN"
                     /locus_tag="OQQ48_003834"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HCS1954424.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINT"
BASE COUNT         1687 a         1587 c         1393 g         1524 t
ORIGIN      
        1 agatattcct caagtaaaaa aacacctctt cctgcgattt ctcacaaaaa agattcgttg
       61 acaaaaagtg acaaaattat gagattccca tcacacattt tgacatcagg aacggtatgc
      121 tgaattcacc aagacgggaa gacaagaggt aaaatttatg acaatgaaca ttaccagcaa
      181 acaaatggaa attactccgg ccatccgcca acatgtcgca gaccgtctcg ccaaactgga
      241 aaaatggcaa acacatctga ttaatccaca tatcattctg tccaaagagc cacaagggtt
      301 tgttgctgac gccacaatca atacacctaa cggcgttctg gttgccagtg gtaaacatga
      361 agatatgtac actgcaatta acgaattgat caacaagctg gaacggcagc tcaataaact
      421 gcagcacaaa ggcgaagcac gtcgtgccgc aacatcggtg aaagacgcca acttcgtcga
      481 agaagttgaa gaagagtagt cctttatatt gagtgtatcg ccaacgcgcc ttcgggcgcg
      541 ttttttgttg acagcgtgaa aacagtacgg gtactgtact aaagtcactt aaggaaacaa
      601 acatgaaaca cacaccgttt ttcttcgcat tcttttttac cttcccctga atgggaggcg
      661 tttcgtcgtg tgaaacagaa tgcgaagacg aacaataagg cctcccaaac cggggggcct
      721 tttttattga taacaaaaaa ggcaacacta tgacatcgga aaatccatta ctggcgctgc
      781 gagagaaaat cagcgcgctg gatgaaaaat tattagcatt actggcagag cggcgcgaac
      841 tggccgtcga ggtgggaaaa gccaaactgc tctcgcatcg cccggtacga gatattgatc
      901 gtgaacgcga tttactggaa agattaatta cgctcggtaa agcgcaccat ctggacgccc
      961 attacattac tcgcctgttc cagctcatca ttgaagattc cgtattaact cagcaggctt
     1021 tgctccagca acatctcaat aaaattaatc cgcactcagc acgcatcgct tttctcggcc
     1081 ccaaaggttc ttattcccat cttgcggcgc gccagtatgc tgcccgtcac tttgagcaat
     1141 tcattgaaag tggctgcgcc aaatttgccg atatttttaa tcaggtggaa accggccagg
     1201 ccgactatgc cgtcgtaccg attgaaaata ccagctccgg tgccataaac gacgtttacg
     1261 atctgctgca acataccagc ttgtcgattg ttggcgagat gacgttaact atcgaccatt
     1321 gtttgttggt ctccggcacg actgatttat ccaccattaa tacggtctac agccatccgc
     1381 agccattcca gcaatgcagc aaattcctta atcgttatcc tcactggaag attgaatata
     1441 ccgaaagtac gtcagcggca atggaaaagg ttgcacaggc aaaatcaccg catgttgctg
     1501 cgttgggaag cgaagctggc ggcactttgt acggtttgca ggtactggag cgtattgaag
     1561 cgaatcagca acaaaacttc acccgatttg tggtgttggc acgtaaagcc attaacgtgt
     1621 ctgaccaggt tccggcgaaa acgacgttgt taatggcgac cgggcaacaa gccggtgcgc
     1681 tggttgaagc gttgctggtg ctgcgcaacc acaatctgat tatgacccgt ctggaatcac
     1741 gcccgattca cggtaatcca tgggaagaga tgttttatct ggatattcag gccaatcttg
     1801 aatcagcgga aatgcaaaaa gcattgaaag agttaggtga aattacccgt tcaatgaagg
     1861 tattgggctg ttacccaagt gagaacgtag tgcctgttga tccaacctga tgaaaaggtg
     1921 ccggatgatg tgaatcatcc ggcactggat tattactggc ggttgtcatt cgcctgacgc
     1981 aataacacgc ggctttcact ctgaaaacgc tgtgcgtaat cgccgaacca gtgctccacc
     2041 ttgcggaaac tgtcaataaa cgcctgctta tcgccctgct tcagcaactc aatcgcctcg
     2101 ccgaaacgct tatagtaacg tttgattaac gccagattac gctctgacga cataataatg
     2161 tcggcataaa gctgcggatc ctgagcaaac agtcgcccga ccatcgccag ctcaaggcgg
     2221 taaatcggcg aagagagcgc cagaagttgc tcaagctgaa cattttcttc tgccagatgc
     2281 agcccataag caaaagtagc aaagtggcgc agagcctgaa taaacgccat attctgatcg
     2341 tgctcgacag cgctaatacg atgcagccga gcgccccaga cctgaatttg ctccagaaac
     2401 cattggtatg cttccggctt acgtccatca caccagacca caacttgctt tgccaggcta
     2461 ccgctgtccg ggccgaacat cgggtgtaac cccagtaccg ggccatcgtg cgccgccagc
     2521 atggcctgta atggtccatt tttcactgat gccagatcaa ccagaataca atctttcggt
     2581 aaaggcggta atttgccgat aacttgctca gtaacgtgga ttggcacact aacaatcacc
     2641 attccggcat cggcaacaat atcagccgct cgatcccagt catgttgctc cagaatccgc
     2701 acctgataac ccgagagtgt cagcatcttc tcgaacaggc gtcccatctg accgccaccg
     2761 ccgacgataa ccaccggacg cagtgacgga caaagtgttt taaatccttt gtcgttttca
     2821 ctggagtaag attcacgcat cacccgacgc aaaacatcct caatcagatc tggcggtacc
     2881 cccagagctt ccgcctctgc acgacgcgag gccaacatag atgcctcgcg ctccggaaca
     2941 taaataggca gtccaaagcg gcttttcacc tcgcccactt cagcaaccag ttccagacgc
     3001 ttcgctaata aattcagcag cgctttatcg acttcatcaa tttgatcgcg taatgcggtc
     3061 aattcagcaa ccataataaa cctcttaagc cacgcgagcc gtcagctgcc cgttcagatc
     3121 ctgatgaatt tcacgcagca aggcatcggt catttcccag ctaatgcagg catcggttac
     3181 ggatacaccg tatttcattt cactgcgcgg ttgctcagaa gactgattgc cctcgtggat
     3241 attactttcg atcatcagac caataattga gcgattgcca tctttgattt gagcaaccac
     3301 ggattctgcc accgcaggct gacggcgata atctttattg gaattaccgt ggctgcaatc
     3361 taccatcaga gacgggcgca gtcccgcctg ttccatctct ttttcacatt gcgcaacatc
     3421 cgcagggcta tagttcggcg ctttaccacc gcgcaggatg acatgcccgt ccggattccc
     3481 ctgagtttgt agcaacgcaa cctgccctgc ctggttaatg ccaacaaaac ggtgcggctg
     3541 ggcggcggcg cgcatagcgt taattgctgt tgccagactg ccgtcggtgc cgtttttaaa
     3601 accaaccggc atggaaagcc cggaggccat ttcacggtga gtttgcgatt ccgttgtacg
     3661 agcaccaatt gctgaccagc taaacagatc gcccaggtat tgcgggctat tcggatctaa
     3721 cgcttccgtc gccagtggca gtcccatatt caccagctca agcagcaatt tacgcgcgat
     3781 ctgcagcccg gcttctacat caaaagagcc atccatatgg ggatcgttaa ttaacccttt
     3841 ccagccgaca gtggtacggg gtttttcaaa atagacgcgc attaccagat agaggctatc
     3901 gctgacctct gcggcaaggg ctttaaatcg acgagcatat tccagagcag tttccagatc
     3961 atgaatggaa caaggaccac aaactaccag cagacgagga tcgcgcccgg cgataatatc
     4021 tgaaatggtt ttacgcgagt cagcaatctg ggcttcttgt tgcaggctca atggaaaagc
     4081 ggccttcagt tgttccggag tcattaaaac ctgttcgtcg gtaatatgta cgttattcag
     4141 cgcgtctttt tgcatgatgg cgatcctgtt tatgctcgtt tgcgatagtt gatcctcagc
     4201 gaggatgacg taacgataac acataaagta aagttttcaa tccatatttc gtacgctttt
     4261 ctttacacga acaatttact cgcgctttga tgcttatttc gtacaaataa atttacaccc
     4321 cctttaaaaa caatccgcta tgctttgaaa aaggagaaag aaatgatgaa aaagtttatc
     4381 gcccccttat tggctttact ggttagcgga tgtcagattg atccttatac tcacgcgcca
     4441 accttgacca gcaccgactg gtatgatgtc ggtatggaag atgcgatatc gggcagcgcc
     4501 ataaaagatg acgatgcatt tggcgattca caggcggatc gcggtctata ccttaaagga
     4561 tatgccgaag gacaaaagaa aacttgccag accgatttta cttatgcccg gggactttcc
     4621 ggtaaaagct ttcctgccag ctgtaacaat gttgaaagtg ccagccaact gcatgaagta
     4681 tggcaaaaag gggctgatga aaatgccagc acaatacgat taaattaatt aaaaccccac
     4741 aaataaatta aacataagat tttactcatt gtgttttatt tctcacattg atgacgatcg
     4801 catgaaaaaa tgatatttta atgagcattc tgaaaaaata tcgatgcatt tcgagcgaag
     4861 aaggtgggga tccctgaatg cgtttttctc accgactgtt cttacttctg attctcctgc
     4921 tgacgggagc accaatactc gctcaggagc cttctgacgt tgcgaaaaac gtgcgtatga
     4981 tggtttccgg tattgtcagt tacacccgct ggcctgcgct atcagggcca ccaaagttat
     5041 gtatcttttc atcttcacgt tttagtaccg ccctacaaga gaatgccgcg acatctttgc
     5101 cttatctgcc tgtcatcatt cacactcaac aagaggcgat gatttcaggc tgcaatggtt
     5161 tttattttgg caacgagtca ccgacattcc agatggaatt aaccgaacaa tatccgtcaa
     5221 aggcgttatt attaattgcc aaacaaaata ccgagtgcat tattggtagc gccttttgtc
     5281 tgatcatcca caataatgac gtcagatttg ccgtaaacct ggatgcctta tcgcgtagcg
     5341 gtgtaaaggt caacccggat gtcttaatgc tcgcacggaa gaaaaatgat ggataacgat
     5401 aattctctta ataagcgccc cacgtttaaa agagcattac gcaacatcag tatgaccagc
     5461 atatttatca ctatgatgct gatctggttg ctgctttccg tgacctcggt gctgaccctg
     5521 aaacagtacg cgcaaaaaaa cctggcactg acagcagcaa caatgactta cagtctggaa
     5581 gcagctgtcg tttttgccga tggccctgca gcaactgaaa cactggcagc gctgggccag
     5641 caagggcaat tttcaactgc agaagtacgt gataagcagc aaaatattct ggcgtcctgg
     5701 cattacaccc gtaaggatcc aggcgatact ttcagcaatt tcataagcca ctggctcttc
     5761 cccgccccca tcattcagcc gattcgtcac aatggtgaaa ccattggcga agtacgctta
     5821 accgctcgcg acagttcaat cagccatttt atctggtttt cgctcgccgt actgaccggt
     5881 tgtattctgc tggcatcagg catcgcaatt accctcaccc gccatttgca caatggccta
     5941 gtggaagcgc tgaaaaatat caccgatgtc gtacatgatg tgcgttccaa ccgcaatttt
     6001 tcccgacgag tttctgaaga acgtatcgct gagtttcacc gcttcgctct cgacttcaac
     6061 agtctgctgg atgaaatgga agagtggcag cttcgtttac aggccaaaaa tgcgcagctt
     6121 ctgcgtaccg cgctacatga cccattaacc gggctggcta accgcgcagc gtttcgtagc
     6181 ggcatcaata c
//
LOCUS       DAJVUJ010000204         6136 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.204, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000204 DAJVUJ010000000
VERSION     DAJVUJ010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6136
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.204"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            26..151
                     /locus_tag="OQQ48_003835"
     CDS             26..151
                     /locus_tag="OQQ48_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001397278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCS1954425.1"
                     /translation="MIGLISALKMPEVKDRDLSALEDAAEDQPRVVRAAQPSRSL"
     gene            240..1700
                     /locus_tag="OQQ48_003836"
     CDS             240..1700
                     /locus_tag="OQQ48_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCS1954426.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYKVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAKAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRYAAYGLMLQEQAP
                     TLKVQGVDLQDYANRLIERYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(1736..1939)
                     /gene="ydfZ"
                     /locus_tag="OQQ48_003837"
     CDS             complement(1736..1939)
                     /gene="ydfZ"
                     /locus_tag="OQQ48_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HCS1954427.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(1936..2802)
                     /locus_tag="OQQ48_003838"
     CDS             complement(1936..2802)
                     /locus_tag="OQQ48_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005005969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCS1954428.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIEHKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVARSA
                     RKTATGLAKSNSFTLIPSGMRGFSPRLTAHACIKKDVSLIKNKKYFTRQEYLYCARYV
                     GFDCKSERRKHL"
     gene            complement(2891..3637)
                     /gene="ydfG"
                     /locus_tag="OQQ48_003839"
     CDS             complement(2891..3637)
                     /gene="ydfG"
                     /locus_tag="OQQ48_003839"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HCS1954429.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            3774..5819
                     /gene="dcp"
                     /locus_tag="OQQ48_003840"
     CDS             3774..5819
                     /gene="dcp"
                     /locus_tag="OQQ48_003840"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HCS1954430.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(5864..>6136)
                     /locus_tag="OQQ48_003841"
     CDS             complement(5864..>6136)
                     /locus_tag="OQQ48_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HCS1954431.1"
                     /translation="LERYSAVSEKVAAEMATGAIERADADVSIAITGYGGLEGGEDGT
                     PAGTVWFAWHIKGQTYTAVMHFAGDCETVLALAVRFAHVQLLQLLL"
BASE COUNT         1538 a         1470 c         1577 g         1551 t
ORIGIN      
        1 cgccattatg atcatggcat attcagtgat tggtttaatc tctgcgctga aaatgccaga
       61 ggtgaaagac cgtgatttaa gtgcgctgga agacgctgcg gaagatcaac cgcgtgttgt
      121 aagagctgcg caaccttcca gaagtctgta aaccctcaat cccttctctt accggagagg
      181 ggatttttat tcatataaca aaacatatag cttgccatat ttaaatttaa ggaattgtta
      241 tgggaaataa tttgttatca gcaaaagcga cactccctgt ttatgatcgt aataacctgg
      301 ccccaagaat tgttcattta ggctttggtg catttcaccg tgcgcatcag ggcgtgtatg
      361 ccgatattct tgctacagaa catttcagtg actggggata ttataaggtc aacttaatcg
      421 gcggcgaaca gcaaattgcc gatttacaac agcaagataa tctttatacc gttgcggaaa
      481 tgtcggccga tgcgtggacg gctcgcgtcg ttggcgtcgt taaaaaagcc ttgcacgtac
      541 agatggatgg cttagaaacc gtgttggctg cgatgtgtga accgcaaatc gcgattgtct
      601 ctctgacaat caccgaaaaa gggtatttcc actctccggc gaccggacag ttaatgctcg
      661 atcacccgat ggtcgctgcc gacgtgcaaa atccccacca gccgaaaaca gcaacagggg
      721 tgattgttga ggcgctggcc cgccgtaaag cggcaggact tcccgcattt accgtcatgt
      781 catgtgacaa catgccagaa aacggtcatg ttatgcgtga cgttgtcact tcatacgcga
      841 aagctgttga tgtaaaactg gcacaatgga tcgaagataa cgtgactttc ccatcaacaa
      901 tggtggaccg tattgtgccc gcagtgacag aggatacgct ggcgaaaatc gaacaactta
      961 ccggtgtgcg cgatcctgcg ggcgttgcct gtgaaccttt ccgccagtgg gtaatagaag
     1021 ataactttgt tgccggacgt ccagaatggg aaaaagcggg agccgaactg gttagtgatg
     1081 tgctgcctta tgaagagatg aagttgcgca tgctcaacgg cagtcattca ttcctggcgt
     1141 atttgggtta tcttgccgga tatcagcaca ttaatgactg catggaagat gaacattatc
     1201 gttatgcggc gtatggcttg atgttgcagg aacaagcgcc gacgctgaaa gtgcagggcg
     1261 ttgatttgca agattacgct aaccgattaa ttgaacgcta tagcaatccg gcgttacgtc
     1321 atcgaacctg gcagattgcg atggatggta gccagaaatt gccacagcgg atgttggatt
     1381 ctgttcgctg gcatctggcg catgacagca agttcgatct gctggcgctg ggcgtcgcgg
     1441 gttggatgcg ttatgtcggt ggtgttgatg aacagggaaa tccgatagaa atcagtgacc
     1501 cactgttacc tgttattcag aaggctgtac aaagtagtgc cgaagggaaa gcgcgcgtcc
     1561 agtcattgct ggcgattaag gcaatctttg gtgatgattt gccagacaat agcttgttta
     1621 ctgcaaaagt gacggaagcg tacttgtctt tattagcgca tggcgcgaaa gcgaccgtgg
     1681 cgaaatattc cgtgaagtaa aaaaagcaat gccgccatcg gcggcattca cacacttagt
     1741 tcatgccgag acgaatcagg tccagaggtg ccagtttctc ttcacaacct tcaacaacta
     1801 cggtttttcc gcggcggatt tgctcagcgg tcagaccttc agtatcaatc gacaggatct
     1861 tgccagtgtg accggtgccg ctaaccataa cacggctgcc agtggtgatt gcgttacggt
     1921 tacgatcgta ggtcgtcata ggtgttttct cctttctgat ttacagtcga agcccacgta
     1981 acgggcgcaa tataaatact cctgtcgagt gaaatatttt ttgtttttga tcaagctcac
     2041 atcttttttg atgcatgcat gagccgtaag gcggggggaa aagcctctca tcccggatgg
     2101 gatgagagta aatgaattac tcttcgctaa accagtcgct gttttcctgg cggatctggc
     2161 cacggattcg ctgatttcct gcaaatgttg cgtcattgca cgttctaccg catcgccatc
     2221 acgtttttgc agggcagaga aaatatcaag atgctggcgt aacagcattt ctggtggaga
     2281 aacatggtcg aaactcatat agcgcacgcg atcaacggtc gctttcaggt tctcaatggt
     2341 atcccacgcc agttgacagt cggcaatctg cgtcaggagt tgatggaagt tgtcatcaag
     2401 ttcaaaaaaa tcatccagtt gcttgtgctc aatggcaatg cgttgctggt gaagattttg
     2461 ttccagttgg tagcactggc tttcggtaat catgctcgcc gcccgacgcg ccaccgcgca
     2521 ctcaatggcc tgacggataa aactgccgtt gcgcacctgg gccatggaaa ttttgttgac
     2581 gtagctgcca cgctgtggac gaatttgaat caggccgttt tccgccagtt taataaaggc
     2641 ttcacgaacc ggctggcgtg acacattgaa acgaacagaa acttcttttt ccgacaacgg
     2701 tgtgcctgga gcaatcaggc aatggacaat gtcgcgacga aggatacgat aaatttgctg
     2761 attgacaggc tgtgtgggat taagttgcgt ttcgacggtc attcgttctt gcttgtgagt
     2821 gagttaactg catgagtcta ccactttttt gtgacaagca ataacccggc agttacgccg
     2881 ggtataaaaa ttactgacgg tggacattca gtccggcata gctttgggta accggcatca
     2941 tttccagggt attgatattg acgtgagcag gcagcgttga cacccaccaa acggcttcgc
     3001 tgacatcttc tggcgtcaat gcaacggtat tttgataggt tttttccgct ttaccgtcat
     3061 cgcctttaaa gcggacattg gaaaactcgg tgccacccac cagacccggt tcgatgtcgg
     3121 tgacgcgcac cgccgtacca tgcaggtccg tacgcagatt caggctaaac tgacgaacaa
     3181 acgctttcgt cgcaccgtaa acgttaccac cggcataagg ccagctacct gccgttgagc
     3241 caatgttaat aatatgacca tgattacgtt caaccatacc cggtaagacg gcgcgcgtca
     3301 tatataccag gcctttgttg ttggtatcaa tcatcgtttc ccagtcttca acgctggctt
     3361 tatgcgcagg ctccatgccc aacgccaggc cggcattatt taccaggata tcaatattgc
     3421 accactcggc aggaagcgat gccagcatct cttcaatagc ggcgcggttg cgaacgtcca
     3481 gttgggcgat gtacagatta tctcccagtt cgtcttttaa ctcctgcaac cgttcctggc
     3541 gacggccagt ggcgataact ttatgccctt gttgaataaa acgacgagta atgcattcac
     3601 caaaacctgc cgttgctcca gttactaaaa cgatcatctc actgttcctc aacgcttttg
     3661 tgtgtcaata acatagcacg tgatgcttaa gagcgggtaa tactggtttg taagattgca
     3721 ctccgttatc agtaagccta cgctatggaa cacctcgcgt tcaggagaag aaaatgacaa
     3781 caatgaatcc tttccttgtg caaagcacac taccgtatct ggctccccat tttgatcaaa
     3841 ttgccaatca tcactatcgc ccggcattcg atgagggaat gcagcaaaag cgggcagaaa
     3901 ttgctgccat cgcgcttaac ccgcaaacgc ctgatttcaa caatactatt ctggcactgg
     3961 aacaaagcgg agaattactt acccgcgtta ccagcgtctt ttttgcgatg actgcggcgc
     4021 ataccaatga tgaattacag cgtcttgatg aacagttttc cgctgaactg gcggaactgg
     4081 ctaatgatat ctatctgaac ggtgaattat tcgcgcgggt agatgctgtc tggcagcgcc
     4141 gtgaatccct ggggcttgat agtgaatcca tccgcctggt ggaggtgatt catcaacgtt
     4201 ttgtccttgc cggagccaaa cttgcgcaag ctgataaagc aaaattaaaa gtactgaata
     4261 cagaagctgc gaccctgacc agccagttta accagcgatt actggcagca aataaatccg
     4321 gcggtctggt tgtgaacgat atcgcgcagc tggcaggaat gagtgagcaa gagattgcgc
     4381 tggcggcaga ggcggctcgc gagaaaggtc tggataacaa atggctgatt ccgctgctga
     4441 ataccaccca acaaccggcg cttgccgaga tgcgcgatcg tgcgacgcgt gaaaaactgt
     4501 ttattgcggg ctggacgcga gcggaaaaaa atgatgccaa tgatacccgc gctatcattc
     4561 aacgtctggt agagattcgc gcacagcagg cgaaactgct tggttttcct cattatgccg
     4621 catggaaaat cgccgatcag atggcaaaaa cgccagaagc agcactcaac tttatgcggg
     4681 aaattgttcc agcggcgcgt caacgtgcta gcgatgaatt agcctccata caggcggtta
     4741 tcgataagca gcaaggcggg tttagcgcgc agccgtggga ctgggcattt tatgccgaac
     4801 aggtacggcg ggagaaattt gatcttgatg aggcgcagct caagccatat tttgaattaa
     4861 acacggtgtt gaatgaaggt gtattctgga ccgcgaatca gctcttcggt attaagtttg
     4921 tcgaacgttt tgatattcct gtctaccatc ctgacgttcg tgtgtgggaa atttttgatc
     4981 ataatggcgt gggactggcg ttattttacg gtgatttctt cgcccgtgat tcaaaaagcg
     5041 gcggtgcatg gatgggcaat tttgttgagc aatcaacgct taatgaaacg catccggtaa
     5101 tttataacgt ttgcaattat cagaaacccg ctgccggtga gcctgcgttg ttactctggg
     5161 atgatgtcat aaccttattc catgaatttg gtcatacgct gcacggcctt tttgcccgcc
     5221 agcgttatgc cacgctttcc ggcaccaaca cgccgcgtga ttttgtcgaa tttccgtcgc
     5281 aaatcaacga acactgggca acgcatccgc aggtattcgc tcgctacgcc cggcattatc
     5341 agagcggggc agcaatgcct gacgaactgc aacagaaaat gcgtaatgcc agcctgttca
     5401 acaaagggta tgagatgagc gaactgctta gcgccgcact tctcgatatg cgctggcatt
     5461 gcctggaaga aaacgaagca atgcaggatg tcgatgattt tgaattgcgg gcgctggtgg
     5521 cggaaaatat ggatcttcct gctataccgc cacgctatcg cagcagttat ttcgcccata
     5581 tttttggtgg cggatatgct gcaggttatt acgcttatct gtggacgcaa atgttggccg
     5641 atgatgggta ccagtggttt gttgagcagg gcggattaac gcgtgaaaat gggcagcgtt
     5701 ttcgcgaggc gatcctttcc agaggtaaca gcgaagatct ggaacgcctg tatcgacaat
     5761 ggcgcggtaa ggctcctcag attatgccga tgctgcaaca tcgtggcttg aatatataaa
     5821 caccaccaat ggctggcctg gagatatcgc caggccagcc tggttatagc agtaattgca
     5881 gcagctggac gtgggcaaac ctcaccgcta aagctaatac cgtttcgcag tcgccagcaa
     5941 aatgcataac cgcagtgtag gtctggcctt taatatgcca cgcgaaccag acggtacccg
     6001 ctggcgtacc atcttcaccg ccctccagtc cgccgtagcc ggtaatggca atactgacat
     6061 cagcatccgc acgctctatg gcacctgttg ccatttctgc tgccactttc tcactcaccg
     6121 cagaatatcg ttcaag
//
LOCUS       DAJVUJ010000205         6053 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.205, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000205 DAJVUJ010000000
VERSION     DAJVUJ010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6053
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.205"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(95..2197)
                     /locus_tag="OQQ48_003842"
     CDS             complement(95..2197)
                     /locus_tag="OQQ48_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCS1954432.1"
                     /translation="MKIFSVRQTVLPALLVLSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTGVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PSPGRNYGVGMNIAWRFE"
     gene            2439..3500
                     /locus_tag="OQQ48_003843"
     CDS             2439..3500
                     /locus_tag="OQQ48_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HCS1954433.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            complement(3613..5112)
                     /gene="ansP"
                     /locus_tag="OQQ48_003844"
     CDS             complement(3613..5112)
                     /gene="ansP"
                     /locus_tag="OQQ48_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HCS1954434.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            5379..5996
                     /locus_tag="OQQ48_003845"
     CDS             5379..5996
                     /locus_tag="OQQ48_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HCS1954435.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQIPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWAPDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGPGHEWFQDNATNISAIADAVCQLPKLQEVLKRNEII"
BASE COUNT         1612 a         1537 c         1444 g         1460 t
ORIGIN      
        1 gtcttgctga aattatgcaa caaaatattg ctattttata ccagcaatac aatcgttaaa
       61 gtaaatgccc ggcaaatacc gggcatcaag gtaattactc aaatctccac gcaatattca
      121 tgccgacacc ataatttcgt ccgggcgaag gttcgtaata tctcccgttt gactcattga
      181 caatgacaga accaacgtat tctttatcga ataaattatc gacacgacca aataaatcca
      241 cagttaaatt gtggtaattg tatttatacc cggtgaataa gccgacgaga gtataagacg
      301 gcgcttttgc cgtattttca tcatctgcca taatatcgcc catataacgc gcttccgtgc
      361 ctgcatacca accatcttcc ggtacataac ctatcgacgc aaagcccata ttacgggcga
      421 tccctggcat ccgattaccg ttacaatcct gttcattgca aacattgctg cgataggtcg
      481 catcaagcca ggtccaggac gcgtttacgc gaaaatctcc tgcgaaacgt tgatcccatg
      541 ccagttcagc gccttgacga cgggtctttc cggcattttt gtaagtcgta cgcccaccgc
      601 tactgctatc gacaacaatt tcatcatcag tgtcggtctg aaacaatgcg agactaagca
      661 gcccatcacc aatacgcgtt ttactgccga tctcaattgt atcgttggtg gatggtttta
      721 aaccaaagtt cataccgctt tgcccatcag cacgataaga cagctcatta atcgtcggcg
      781 tttcaaaacc tcgcccggct gccagataga tattccaggc atcggtcatt gcatatttta
      841 acgaaccggc aggtagccat ttatgataac tggcatcacc gctgtcatcg ccgttacccg
      901 gagtaacgta atggtcgttg gaatcaaacc acacggagct gtagcgcacg ccagcatcca
      961 gcgacagttt ttcgctcagc tgccactgcg tctgtaaata gggatcgatg ttccacatca
     1021 gattgcgttc gtcgcgacgc aactcacctt tttgcccgta ctccggcatg ccgctattca
     1081 ggcggaagtt attgtagccc ttgcggtttt cactcatgtt ttcgtagttc aggccggtag
     1141 tgaacgtgac cggaacgccc agttcaccac ggtgtgtcca gcggctgtct atcccctggt
     1201 aatggcgttg cagggtaatc acgccgcccg catgtgacgg gttaagttgt ggtgccatgg
     1261 gtattgactg gtactgggtc gtttctcgct ctccggcata catcatcaca ctcatatcat
     1321 cttgcgcgct caggctacgc tcatagcgca acccagcctg agtttgcttg atggtttttc
     1381 gcgtgtcgta ctgttctgca cgaggcgctt gttgtggatt agccttccat tctgctttgg
     1441 ttagcccacc tgggtcatct gctttgatat ccacactatt gaaaatcaga cttaatttgc
     1501 tggcttcatc aatgcgtacg cccagtttgg cattggctaa atttttctgt gcgccactat
     1561 ggtcacgata gccgtgggtc gtaaaacgcg tggttgagac ggtgtaatcg acatcgccag
     1621 gctgtgtgcc gtctcccgtt gcgcccgttg ctttcagccc atagcgccag ctgccaaaac
     1681 tgccgtagta actactggct tcaatggttg gtggctgttg tccggtctgg gtggtgacat
     1741 tcattacccc accagacgcg ttgccataca gggcagagaa ggggccacgc agcacttcca
     1801 cattttgcac actgcttaaa tcgatgttgg atgtttgccc ttgcccgtcg ggcatggtgg
     1861 cgggaatacc gtccacatac aggcgaatac cgcgaatacc gtaagtggag cgggagccaa
     1921 atccgcgaat cgacagctgt aaatcttgcg catagttctg ccggttttgt acctgcaaac
     1981 caggcacgcc ggtcagtgat tcggacaagt taatgcgcgg tgttgccagg cgcatctcct
     2041 cgccatccac cacgcttact gctgctgggg tatccagttc tgaaaccacc tgcggtgcgg
     2101 cactgacaat catagtctgt tcatcagcgg caaaaacaac gggggaaagg acaagcagtg
     2161 cgggcaaaac ggtctgtcgg acggaaaaaa tcttcatgaa aaaagccagg ttaagaatgg
     2221 gaaaacgctg tcatggtaat aaaattgtaa acttatgcaa aacgaaacgg cacgatacgt
     2281 taattaattg agaaaattgt agtcgtaacg gcaagaaatg ctccacattt gagaaaataa
     2341 tgattaccat tcccatttat aacaagagcg tgacgatgat tacgcttagc gaagcattgt
     2401 gaagcagcaa aaatattggt tcatcaaagg gagtcgtcat gcatttacgt catctgtttt
     2461 catcgcgcct gcgtggttca ttactgttag gttcattgct tgttgcttca tcattcagta
     2521 cgcaggccgc agaagaaatg ctgcgtaaag cggtaggtaa aggtgcctac gaaatggctt
     2581 atagccagca agaaaacgcg ctgtggctcg ccacttcgca aagccgcaaa ctggataaag
     2641 gtggcgtggt ttatcgtctt gatccggtca ctctggaagt gacgcaggcg atccataacg
     2701 atctcaagcc gtttggtgcc accatcaata acacgactca gacgttgtgg tttggtaaca
     2761 ccgtaaacag cgcggtcacg gcgatagatg ccaaaacggg cgaagtgaaa ggccgtctgg
     2821 tgctggatga tcgtaagcgc acggaagagg tgcgcccgct gcaaccgcgt gagctggtag
     2881 ctgatgatgc cacgaacacc gtttacatca gtggtattgg taaagagagc gtgatttggg
     2941 tcgttgatgg cgagaatatc aaactgaaaa ccgccatcca gaacaccggt aaaatgagta
     3001 ccggtctggc gcttgatagc aaaggcaaac gtctttacac cactaacgct gacggcgaat
     3061 tgattaccat cgacaccgcc gacaataaaa tcctcagccg taaaaagctg ctggatgacg
     3121 gcaaagagca cttctttatc aacatcagcc ttgataccgc caggcagcgt gcatttatca
     3181 ccgattctaa agcggcagaa gtgttagtgg tcgatacccg taatggcaat attctggcga
     3241 aggttgcggc accggaatca ctggctgtgc tgtttaaccc cgcgcgtaat gaagcctacg
     3301 taacgcatcg tcaggcaggt aaagtcagtg tgattgacgc gaaaagctat aaagtggtga
     3361 aaacgttcga tacgccgact catccaaaca gcctggcgct gtctgccgat ggcaaaacgc
     3421 tgtatgtcag tgtgaaacaa aaatccacta aacagcagga agctacccag ccagacgatg
     3481 tgattcgtat tgcgctgtaa tagatatggc gagggtgaaa acggtgagac ttgcctgcct
     3541 tttatcacgg cgtatccggc actttcgtca gtaatctgaa tttgcccggc acgtagccgg
     3601 gcaattttgc aattaactcg ccgtttcagg cttaaacaca atttcctgtt tttcttcatc
     3661 ttcctcgacg actggcgcag tgctgtgaat ttcagcaacg cgtttgcgca caccaaacca
     3721 gcctataacc agcagaatac cgataatcgg cagcgccgcg atagtataag tcccgttcgg
     3781 gtaatcgaac gccatcagca caaggacact cagtaaaaac agtaatgtca gccaggaagt
     3841 gaagggcgcg ccaggcagtt taaaactgac atccgctgct ttgccttctt taatcgcttt
     3901 acgcaggcgc atctggcaca cgatgataaa cgcccatgaa gcgatgattc ccagcgacgc
     3961 gaagttcaac acaatctcaa atacgcgcga cggcaccaga tagttgagga atacgccgac
     4021 gacatacaca accagtgtcg ctagaatccc ggcatacggc acatgctgac gactcatttt
     4081 cgccataaaa ctcggtgcgg aaccgcccat cgccattgag cgcagaatac gtccagtgca
     4141 gtacagacct gagttcaggc tggagagggc agcggtcagc accacaatgt tcataatgct
     4201 gccgatatat ggcacaccca gtttagagaa aaacgtcacg aacggacttt gccccgcctg
     4261 atacgcgctc cacggcaata acataaccag caacaccacg gagccgacgt aaaacaggcc
     4321 aatacgccaa atcacactgt taatggcttt aggcaccatg gtctgcggat ctttacattc
     4381 tccggcagct gtacccacca tttcaatgga ggcaaaagca aacactacgc cctgaatcaa
     4441 caccagcgca ggcagcaaac cgtgggggaa gaagccgcca ttatcggtga ttaaatgaaa
     4501 gccagtggtg ttgccatcca gcggctgacc actaccgagg aacactgtac ccacgaccaa
     4561 aaaggtcaca atggcgagca ctttaataag cgcaaaccag aactccatct ccgcaaacca
     4621 tttcacaccg atcatattca tggtgccaac gatggtaagt gcagcgagcg caaagaccca
     4681 ctgcggcacg ccgccaaacg caccccagta atgcatatac agagcgacgg cggtaatatc
     4741 aacaatcccc gtcatcgccc agttgatgaa gtacatccag ccagcaacat aagcggcttt
     4801 ctcacccaaa aactcacggg cataagagac aaaactgcca ctggaagggc ggtgtagcac
     4861 cagctcaccc aatgcacgca gaataaaaaa cgaaaacaag ccacaaatta aataaaccag
     4921 tgccagtgct ggacccgcca tttgcagtcg ggctcctgca cctaaaaaca agccggtgcc
     4981 aatcgcgccg ccaatggcga tcatctgtac ctggcgattg cccatcgctt tgtgataccc
     5041 ctcttcgtgg gcattaagcc agcggcgttt cgcggcgtgt tgatctgaag tgtcggtgtc
     5101 gtgtttactc attgctctcc ctgattgctt taatgaaaaa gtcatataag ttgccatgaa
     5161 caatgtttat tcatcctggc gatgatagtt atcaccatta ttctttatgg gtataaaact
     5221 atcggcgcag catcctaccc gttatcgata tacgatgcaa aacatcccct tacaatcctg
     5281 aaggggattc atacaactga cgaaaaaatg acaaatactt ttgctggtta acctgtgtac
     5341 tgtcctacac ttaatcttta aaagattgtg aggggcatat gattaaggtg tatggcgtac
     5401 ccggatgggg ctctacaatc agtgagctga tgctaacact ggctgatatt ccttatcagt
     5461 tcgttgatgt cagtggtttt gaccacgagg gaacttcacg cgagttattg aaaaccctga
     5521 acccgttgtg tcagattccg acactggcgc tggaaaatga cgagattatg acggagacag
     5581 cggcgattgc attgatggtt ctcgatcgtc gcccggacct tgcgccaccg gttgggcgcg
     5641 ccgaacgtca gcagtttcaa cggctattgg tctggctggt tgctaatgtc tatccaacat
     5701 tcactttcgc cgattaccct gagcgttggg ctcctgacgc acctgaacag ttaaagaaga
     5761 atgttattga atatcgtaaa tcgctttata tctggctgaa ttcacaattg accgctgaac
     5821 cttatgcgtt tggtgagcaa ctaacgctgg tggactgtta tctttgcact atgcgcacat
     5881 gggggcctgg gcatgaatgg tttcaggata atgccacgaa tatcagtgcg attgctgatg
     5941 ctgtatgtca gttaccgaaa ttacaagaag tattaaaaag gaatgaaatt atttgagtgg
     6001 gataccagag gattatgtta tttattattc agtaacttta tccacaatga tgg
//
LOCUS       DAJVUJ010000206         6050 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.206, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000206 DAJVUJ010000000
VERSION     DAJVUJ010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6050
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.206"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(25..393)
                     /locus_tag="OQQ48_003846"
     CDS             complement(25..393)
                     /locus_tag="OQQ48_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1954436.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQITTKMLFMCRE"
     gene            919..1614
                     /gene="minC"
                     /locus_tag="OQQ48_003847"
     CDS             919..1614
                     /gene="minC"
                     /locus_tag="OQQ48_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HCS1954437.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            1638..2450
                     /gene="minD"
                     /locus_tag="OQQ48_003848"
     CDS             1638..2450
                     /gene="minD"
                     /locus_tag="OQQ48_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HCS1954438.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            2454..2720
                     /gene="minE"
                     /locus_tag="OQQ48_003849"
     CDS             2454..2720
                     /gene="minE"
                     /locus_tag="OQQ48_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HCS1954439.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(3199..3318)
                     /locus_tag="OQQ48_003850"
     CDS             complement(3199..3318)
                     /locus_tag="OQQ48_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCS1954440.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(3279..3464)
                     /locus_tag="OQQ48_003851"
     CDS             complement(3279..3464)
                     /locus_tag="OQQ48_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1954441.1"
                     /translation="MSRRYSSATITLVILFCRRPHCRKISVRQYYLKRVIFYPTTLQR
                     TAFKKCLFLMRDIIISS"
     gene            3565..3738
                     /locus_tag="OQQ48_003852"
     CDS             3565..3738
                     /locus_tag="OQQ48_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1954442.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVRATYLIPDI"
     gene            3740..4084
                     /locus_tag="OQQ48_003853"
     CDS             3740..4084
                     /locus_tag="OQQ48_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HCS1954443.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            4088..4423
                     /locus_tag="OQQ48_003854"
     CDS             4088..4423
                     /locus_tag="OQQ48_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HCS1954444.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(4480..6050)
                     /locus_tag="OQQ48_003855"
                     /pseudo
     CDS             complement(4480..6050)
                     /locus_tag="OQQ48_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT         1662 a         1396 c         1385 g         1607 t
ORIGIN      
        1 atagaacata atgtaaacaa ggcatcactc acgacacata aataacatct ttgtggtgat
       61 ttgattgatt tttccactcc gcttaccatc cacaccaaaa taacgatcat cacgacataa
      121 tttttcttta acatcgcaaa atacaccgtt agcgaatgta aattcgctgg gatcgtagcc
      181 ttgtaatgat tgtaagtttt cagcggcttt ttcgccgaga tatttccttg ttaactctgg
      241 ggaaatgcca tctgagttca cacaaatata cgggtcgcaa acaacgccgc acgccggaga
      301 ttgaagtccg ccagcgtcat gtccagacgc cattgatgag aacatcatcc ccacacctga
      361 caatccgata taaaaaatac gcatcatatt catagcacat cctgataatc caaccgtaca
      421 acacttatgc tcatttcatc atcaatccgt aatcaaaacc gctgacctgg acgagttacg
      481 atgacctgac agaagtatca gacgtcttct gcggtttcta tgattatgat agagcaattc
      541 tatggcggat attaagataa cgggtatctg ttctttaatg atgctgcaaa aaatggaaag
      601 ttaagaaagc ctggcgtggt gaaaaaatac acttgcctca atactggata tttaaacagg
      661 tctggagatg ctgtcacgat tagggtgcgg aaaaaggaag cgtaaataag aacaatcaaa
      721 agtacgacgg caatgggttg attgacaagg gtatttttta agctatgaat cagcgccatt
      781 tatcacagaa tagactttta ctctgaataa atgggagggt gacttgcctc aatataatcc
      841 agactataac atgccttata gtcttcggaa catcatcgcg cgctggcgat gattaatagc
      901 taattgagta aggccaggat gtcaaacacg ccaatcgagc ttaaaggcag tagcttcact
      961 ttatctgtgg ttcatctgca tgaggcagaa cctaaggtta tccatcaggc gctggaagac
     1021 aaaatcgctc aggcccccgc atttttaaaa catgcccccg ttgtactcaa cgtcagtgca
     1081 ctggaagacc cggtaaactg gtcagcgatg cataaggcgg tgtcggcaac cggtttgcgg
     1141 gttattggcg taagtggctg caaagatgcg caacttaaag ccgaaattga aaagatgggg
     1201 ctgcctatcc tgacggaagg aaaggaaaaa gcgccgcgtc cagctcccgc accacaggct
     1261 ccagcgcaaa atacaacgcc ggtcacaaaa acgcgtttaa tagatacccc ggtgcgttcc
     1321 ggtcagcgta tttatgctcc acaatgtgat ctgattgtta caagccacgt tagcgctggg
     1381 gccgaattga ttgccgatgg gaacattcat gtctatggca tgatgcgcgg tcgtgcgctt
     1441 gcaggggcaa gtggtgaccg ggaaacgcaa atattttgta cgaacctgat ggcggaactg
     1501 gtgtccatcg caggtgaata ctggctgagt gatcaaatcc cagcagaatt ttatggcaaa
     1561 gcggcgcgac tgcagttagt cgaaaacgct ttgaccgttc aaccgttaaa ttgatccctt
     1621 tttaacaagg aatttctatg gcacgcatta ttgttgttac ttcgggcaaa gggggggttg
     1681 gtaagacaac ctccagcgcg gccatcgcca ctggtttggc ccagaaggga aagaaaactg
     1741 tcgtgataga ttttgatatc ggcctgcgta atctcgacct gattatgggt tgtgaacgcc
     1801 gggtcgttta cgatttcgtc aacgtcattc agggcgatgc aacgctaaat caggcgttaa
     1861 ttaaagataa gcgtactgaa aatctctata ttcttccggc atcgcaaaca cgcgataaag
     1921 atgccctcac ccgtgaaggg gtcgccaaag ttcttgatga tctgaaagcg atggattttg
     1981 aatttatcgt ttgtgactcc ccggcaggga ttgaaaccgg tgcgttaatg gcactctatt
     2041 ttgcagacga agccattatt accaccaacc cggaagtctc ctcagtacgc gattctgacc
     2101 gtattttagg cattctggcg tcgaaatcac gccgcgcaga aaatggcgaa gagcctatta
     2161 aagagcacct gctgttaacg cgctataacc caggccgcgt aagcagaggt gacatgctga
     2221 gcatggaaga tgtgctggag atcctgcgca tcaaactcgt cggcgtgatc ccagaggatc
     2281 aatcagtgtt gcgcgcctct aaccagggtg aaccggtcat tctcgacatt aacgccgatg
     2341 cgggtaaagc ctacgcagat accgtagaac gtctgttggg agaagaacgt cctttccgct
     2401 tcattgaaga agagaagaaa ggcttcctca aacgcttgtt cggaggataa gttatggcat
     2461 tactcgattt ctttctctcg cggaagaaaa acacagccaa cattgcaaaa gaacggctgc
     2521 agattattgt tgctgaacgc cgtcgcagcg atgcagaacc gcattatctg ccgcagttgc
     2581 gtaaagatat tcttgaggtc atttgtaaat acgtacaaat tgatcctgag atggtaaccg
     2641 tacagcttga gcaaaaagat ggcgatattt ctattcttga gctgaacgtg accttaccgg
     2701 aagcagaaga gctgaaataa gcccgctgta aaaacgcatt tatcttcaag gcagagtcat
     2761 ttctgccttg agtttttcat ccctctcatc cacgttgtgg taaagcggcg agtattcttg
     2821 ctgatactcc tcattgctat ttccacctcc ccctttacac cttaaggctg ttcccacgca
     2881 cagaacaaaa tgtttatgcc tggttgagta aataacctca ttgttagtgt ggatacactg
     2941 ggggatgata accccgcaat ttcattattc attgttctcc cataggatga ggtttcccgc
     3001 cgtaactggc gggctttttt tgccccagaa ttttcccttt tagccccctg taaacgaaaa
     3061 ctgcgccgaa gcgcagttta atcgttgatg gtcagaacga ccagttcacg atatatactg
     3121 cgggtatcga ctgatttcga ttccatgcca ttggataaat gatagtagcg tctaagtgac
     3181 ctaagctctg ctgcaacatc agcccgcagg caccagacca ggggattcat cctgaagaga
     3241 cagcgcaggt gtattgtgtt caccactcac caaagacatc atgatgaaat gatgatatcc
     3301 cgcataagaa agaggcattt tttaaacgca gtgcgctgaa gtgtagttgg ataaaaaatc
     3361 actcttttca gataatactg tcgtacgctt atctttcgac aatgaggccg tcggcaaaat
     3421 aaaatgacta atgtaatcgt tgctgatgaa tatcttctgc tcacataaaa atcacacaat
     3481 aatattgata aatcagatta tcttaccttt acaacattgc gttaacagtc aatgacactt
     3541 cttgaataaa aatattgcta atcaatgagt tattcctcat ttaagattat tctcattcac
     3601 gtaaaaaaca taattcctat catcacagcc acgctcattt tgaattatct gaataatagc
     3661 gagcgttctt tagttaagca gatcttaatt gaagacgaaa ttattgtgcg tgcaacttac
     3721 ttaataccgg acatctaagt tgaagaaaaa aatacttgcc ttcgggctta tctcagcatt
     3781 gttctgctcc acgccagcga tggcggatat gaatcgcacc acgaaaggtg cattgctagg
     3841 cgcaggcgtt ggtttactta ctggcaatgg tgttaatggc gtactgaaag gtgcagctgt
     3901 tggcgctggt gttggtgcag taacagaaaa aggccgcgac ggtaaaaatg cgcgtaaagg
     3961 tgcaaaagta ggtgccgccg ttggtgctgt cactggcgtt ctgaccggca atggcctcga
     4021 aggggcgatt aaaggtgccg tgattggcgg tacgggtggt gcaattctgg gtaaaatgaa
     4081 ataaggaatg atgatgaaaa aatttgcttt actggcgggc ctgtttgttt tcgcaccaat
     4141 gacctgggcg caggactaca atattaaaaa cggtttaccg tcagaaacct atattacttg
     4201 cgcagaagcc aacgaaatgg caaaaactga cagcgcacag gttgcagaaa ttgttgcggt
     4261 aatgggtaat gccagcgttg ccagccgtga tttaaaaatt gagcaatctc cggaactgag
     4321 cgccaaagtg gtagaaaaac tgaatcaggt ttgcgctaaa gacccgcaaa tgctgttgat
     4381 cacggcaatt gatgacacca tgcgtgctat cggcaaaaaa taatatttca tatgtaccgc
     4441 gtcgatgaat acgacgcggt tttttagcta acggcgggat cagaacttat atttcaggcc
     4501 caccatcact gcggtgtcat tgtagccatt atcacccagc tgcacgccca cattccccca
     4561 gacgctggcc gctggattca ggttgccatt tacgccaagt ttaatttccc ccaaattact
     4621 cgccccatct tgttcgactt tcacaccgtt cattgagaca gcaaaatctt tactgttatg
     4681 gatccagttc atttcaacaa aaggttcaaa gtcgtcaccg tgcggaccgc tgtttttctc
     4741 ctgagtacga ataaaggtgc ggaaaccaag tcgggtctgg atgttgttat ttgcgtcatt
     4801 ctcaatacgt gagccattgg cttcggtgtg cgcattatga tcgacgccca tccagatagc
     4861 ctgtgcctgt ggctggaata tccacgtgta tttcgccgct tccagaccaa aggatttatt
     4921 cagggcaaag ccctaacccg cttccagcga gacggttgcg cctttcgcac tataggattc
     4981 ataggctaat tcatcccctt tcaccgagtt tttaaaccag ctatattgcg cccaggcgtc
     5041 aatgtatgcg ccttttttac tgatgtcatc ggcaaaccaa gtggcataca gccctgcgca
     5101 atagcctctg acactccctt tcgaacgata atccgacacg ctggaatgag ttgagttgta
     5161 gtcgcgggca taaccagcca tcactcccaa acgccaactg tcactgtcgg taaaaccacc
     5221 ggttaacaga tcggccccta actgcgagac gtagcgatgc gaggtcgttc tcaactgtcc
     5281 gttgctgtca cgccaggcat tatgcccgcc aatttgacgt agccaaagtc ggcttgaacg
     5341 ctcctgttca gttacaggat cgatgtagcg catttcaccc gcacggtcgt tcagacgcat
     5401 aacaaaaaga gtgttagcgg ctgccaggtt ggcgacgtaa cttccagctt cggggcgcag
     5461 gacgggggta ggcgtttctg ttggttctga atttccctct gagctgttat cctcctcatt
     5521 cactgactga taacttgtca aataccagtt tttgccttgt tgttttagtt gatattcgaa
     5581 agctccagct gcaatccttg catcaccttg tctgaaggct tctgctggag attcatcgcc
     5641 attcacagtt atgagttcga tcccgtttag cgtggccgcg ccgcttcctc cagcattttt
     5701 cacatagacg gtagttgaac ccgaagtatt accctcaaca ataagtctgt ccgtagccga
     5761 tttatcatca cctaatacgg tgcggatatt aagctgacca ttattaccga cataattacc
     5821 ttttacggtt aatgtttttc cttgggtaaa tgtttgacca tcatcatacg aaagattgat
     5881 aatactgtct gaattagtca gatgggtaac aacggagtca tctttcatat tccagattgt
     5941 attttctgaa agcttcagcg tggttgtgcc gctaacgagt ttagtcgctc catttagaat
     6001 agagtcggac gaaattatgt ttaagtcacc tccagtatta ttaaccagaa
//
LOCUS       DAJVUJ010000207         6045 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.207, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000207 DAJVUJ010000000
VERSION     DAJVUJ010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6045
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.207"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(85..621)
                     /gene="ssb1"
                     /locus_tag="OQQ48_003856"
     CDS             complement(85..621)
                     /gene="ssb1"
                     /locus_tag="OQQ48_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HCS1954445.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            875..3697
                     /gene="uvrA"
                     /locus_tag="OQQ48_003857"
     CDS             875..3697
                     /gene="uvrA"
                     /locus_tag="OQQ48_003857"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCS1954446.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(3732..4088)
                     /locus_tag="OQQ48_003858"
     CDS             complement(3732..4088)
                     /locus_tag="OQQ48_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCS1954447.1"
                     /translation="MTISELLQYCMAKLGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(4092..4508)
                     /locus_tag="OQQ48_003859"
     CDS             complement(4092..4508)
                     /locus_tag="OQQ48_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HCS1954448.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMQGTSLVL
                     PLHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(4619..5332)
                     /gene="aphA"
                     /locus_tag="OQQ48_003860"
     CDS             complement(4619..5332)
                     /gene="aphA"
                     /locus_tag="OQQ48_003860"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HCS1954449.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            5733..5955
                     /locus_tag="OQQ48_003861"
                     /pseudo
     CDS             5733..5955
                     /locus_tag="OQQ48_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1492 a         1521 c         1530 g         1502 t
ORIGIN      
        1 cataacggag gcttcttttt tatggctggc agatgcttta atcatccacc ttaaaacaat
       61 ataacctatt gttttaatga caaatcagaa cggaatgtca tcatcaaagt ccatcggcgg
      121 ctcgttagac ggcgctgccg gagcggactg ctgcgggcga gactgcgcgc cgccgctgaa
      181 ctgattgcca ccctgcggct gctgaggctg accccaaccg ccctgcggct gaccaccacc
      241 gatattacca cctgccggag cgccaccacc ctgacgacca cccagcatct gcatggtgcc
      301 gccaacgttc accacgactt ctgtggtgta gcgatcctga ccggattgat cggtccattt
      361 acgggtacgc agctgacctt cgatataaac ctgagaacct ttacgcagat attcgctggc
      421 cacttctgcc agtttgccga acagcacaac gcggtgccat tcagtctgtt ctttcatctc
      481 gccggtcgct ttatcacgcc aggattcgga agtagccagc gtaatgttgg caactgcgcc
      541 accatttggc atgtagcgta cttccgggtc ctgacccaga ttaccaacga gaataacctt
      601 gtttacgcct ctgctggcca tgttcgtgtc tcctgaaaaa aatcgttctg aataagtgta
      661 aacgcgcgat tgtaccatta ccaatagcgc ttttactatg ttgtgacctc ggttccggga
      721 aacaaacctg gccagacatt gttacacaac actccggata atgcattcca atactgtata
      781 ttcattcagg tcaatttgtg tcataattaa ccgtttgtga tcgccggtag caccatgcca
      841 ccgggcaaaa aagcgtttaa tccgggaaag gtgaatggat aagatcgaag ttcggggcgc
      901 ccgcacccat aatctcaaaa acatcaacct cgttatcccc cgcgacaagc tcattgtcgt
      961 gaccgggctt tcgggttctg gcaaatcctc gctcgctttc gacaccttat atgccgaagg
     1021 gcagcgccgt tacgttgaat ccctttccgc ctacgcgcgg cagtttctgt cactgatgga
     1081 aaagccggac gtcgatcata ttgaggggct ttctcctgcc atctcaattg agcagaaatc
     1141 gacgtctcat aacccgcgtt ctacggtggg gacaatcacc gaaatccacg actatttgcg
     1201 tttgttgttc gcccgcgtcg gcgaaccgcg ctgtccggac cacgacgtcc cgctggcggc
     1261 gcaaaccgtc agccagatgg tggataacgt gctgtcgcag ccggaaggca agcgtctgat
     1321 gctactcgcg ccaatcatta aagaacgcaa aggcgaacac accaaaacgc tggagaacct
     1381 ggcaagccag ggttacatcc gtgctcgtat tgatggcgaa gtctgcgatc tttccgatcc
     1441 gccgaaactg gaactgcaaa agaaacacac tattgaagtg gtggttgatc gcttcaaggt
     1501 gcgtgacgat cttacccaac gtcttgcgga gtcgtttgaa accgcgctgg agctttccgg
     1561 tggtacagcg gtagtggcgg atatggacga cccgaaagcg gaagagctgc tgttctccgc
     1621 caacttcgcc tgcccaattt gcggctacag tatgcgtgaa ctggagccac gactgttttc
     1681 gtttaacaac ccggcaggtg cctgcccaac ctgtgacggc cttggcgtac agcaatattt
     1741 cgatcctgac cgcgtgatcc aaaaccccga gctgtcactg gctggcggtg cgatccgtgg
     1801 ctgggatcgc cgcaacttct attacttcca gatgctgaaa tcgctggcag atcactataa
     1861 gttcgacgtc gaagcgccgt ggggcagcct gagcgcgaac gtgcataaag tggtgttgta
     1921 cggttctggc aaagaaaaca ttgaattcaa atacatgaac gatcgtggcg atacctccat
     1981 ccgtcgtcat ccgttcgaag gcgtgctgca caatatggag cgccgttata aagagacaga
     2041 atccagtgcg gtacgtgaag aattagccaa gtttatcagc aatcgcccat gcgccagctg
     2101 cgaagggacg cgtctgcgtc gggaagcgcg ccacgtttat gtcgagaata cgccgctgcc
     2161 tgctatctcc gacatgagca tcggtcatgc gatggaattc ttcaacaatc tcaaactagc
     2221 tggtcagcgg gcgaagattg cagaaaaaat ccttaaagag atcggcgatc gtctgaaatt
     2281 cctcgttaac gtcggcctga attacctgac actttcccgc tcggcagaaa cactttccgg
     2341 cggtgaagcc cagcgtatcc gtctggcgag ccagattggt gcgggcctgg ttggcgttat
     2401 gtatgtactg gacgagccgt ctatcggcct gcaccagcgc gataacgagc gcctgttggg
     2461 tacgcttatc catctgcgcg atctcggtaa taccgtgatt gtggtggagc atgacgaaga
     2521 cgcaattcgc gccgctgacc atgtgatcga tatcggtcct ggtgcgggtg ttcacggcgg
     2581 tgaagtggtc gcagaaggtc cgctggaagc gatcatggcg gtgcctgaat cgttgaccgg
     2641 gcagtacatg agcggtaaac gcaagattga agtgccgaag aaacgcgttc cggcgaatcc
     2701 agaaaaagtg ctgaagctga caggtgcacg cggcaacaac ctgaaggacg tgacgctcac
     2761 gctgccagtc ggtctgttta cctgcatcac aggggtttca ggttccggta aatcgacgct
     2821 gattaacgac acactgttcc cgattgccca acgccagttg aatggtgcga ccatcgccga
     2881 accggcaccg tatcgcgata ttcaggggct ggagcatttc gataaagtga tcgatatcga
     2941 ccaaagccca attggtcgta ctccacgttc taacccggcg acctataccg gcgtgtttac
     3001 acctgtgcgc gaactgtttg cgggcgtacc ggaatcccgt gcgcgtggtt atacgccagg
     3061 acgtttcagc tttaacgtcc gtggcgggcg ctgcgaagcc tgtcagggcg acggtgtgat
     3121 caaagtggag atgcacttcc tgccggatat ctacgtgccg tgcgatcagt gcaaaggtaa
     3181 acgctataac cgtgaaacgc tggaaattaa gtacaaaggc aaaaccatcc acgaagtgct
     3241 ggatatgacc atcgaagagg cgcgtgagtt ctttgatgcg gtgccagctc tggcgcgtaa
     3301 gctgcaaacg ttgatggacg ttggtctgac gtacattcgc ctggggcagt ccgcaaccac
     3361 actttctggt ggtgaagccc agcgcgtgaa gctggcgcgt gagctgtcaa aacgcggcac
     3421 cgggcagacg ctgtatattc tcgacgagcc gaccaccggt ctgcacttcg ccgatattca
     3481 gcaactcctc gacgtgctgc ataaactgcg cgatcagggc aataccattg tggtgattga
     3541 gcacaatctc gacgtgatca aaaccgctga ctggattgtc gacctgggac cagaaggcgg
     3601 cagtggcggc ggcgaaatcc tcgtctccgg tacgccagaa accgtcgcgg agtgcgaagc
     3661 ttcgcatacg gcacgcttcc tcaagccgat gctgtaatcg ttaaggccgc tttctgagcg
     3721 gccttttcct ttcagagttg taccagcaat ttacgctttt cttccggtag taaattcact
     3781 gcctgctgat aagacgcatc caccagataa tagatttgcg aatccggcag cgaaccgtcg
     3841 agatacacgg tgctccagtg cgctttattc agatggcggc taggacgcac atcgctgtgc
     3901 tgctgacgta gcagctccgc cagctccggg ctggttttca gcgaaacagc tgggcgattt
     3961 tctacttctt tcaccatcgc aaacagtaca tcttccactt tgatctgcgt cgctttccag
     4021 tcgttatgca cgctctgttc tgcgcctagt tttgccatgc aatattgtag caactccgaa
     4081 atggtcattt tttactcccc ttgtagtgtc gcgatgatgc gacgcgatcc gccgtggatg
     4141 cgatgttccc ccagccagat gccttgccag gtgccggtct gaatacgccc tttatgtaac
     4201 ggcaatacaa gcgatgttcc ctgcattgag gatttgatat gagaaggcat atcgtctgct
     4261 ccctcatagt catgctcata atttccgttg tcgggaacgg tgcggaggaa aaaacgctcc
     4321 atgtcgtggc gtacggtggg atcgcagttc tcattaagtg tcagagaggc ggaggtatgt
     4381 tgcagcaaca gatgcagtaa gccgatgtta acgcgcggca tatcagccag ctgattcaga
     4441 atttcatccg ttaccagatg aaacccacga gatttggcgc taagcgtgag cgtcttttga
     4501 taccacatgt gctgctcctt gataaaactc tcttaatcag tttgcagcaa gacagcgaaa
     4561 ggataaaggt gtgattaaaa aaacagcatt cgggagagca tcacgctctc ccgctctgtc
     4621 agtattctga attgacgatc acctcttcac caaacgcacc cgcttgtggc aagggtttgt
     4681 aggtagagtt ggaggcgcgc agaatgcgga taccacgagc gccgacatcg cgtgcggcgg
     4741 taatatcatt atcagaatcg ccataaaaaa ttcggatatt tttatcctgc agccattgcg
     4801 attttgtatt ttgccctggt ttatcgcccg caaagatcac cggattcatg ttggtggcag
     4861 gaatatgaaa attatccgcc agcgtttttg aaaccgtttc tgttttcgtc ggactacgac
     4921 cagtcacaaa gaagatcgcg tcaccgcggc gtacatgcat atcaatcagc tggcgagcga
     4981 cctcttttgg aatgctgaat tcatcccagc cattgttcat tttttcccag aacacaggat
     5041 ttttcagata atcttcgctt tctggcgaga aggttttttt gccgcgccag aagcccgggc
     5101 tggaaaaaag taccgtgtca tcgatatcaa accccaccgc cattggcgga cgccctgcga
     5161 ggctattttc aatttgtgcg accgaaaccc aatgaattgg tgcctgttca gcaagcctgg
     5221 caacgttagt accagggtta agcggtgaag gagatgaggc cagggcaaca gcggaactgt
     5281 ttagagcgaa caataagcaa acggcactga ttgcctgtgt gatcttgcgc atatttttcc
     5341 ctaaatagtc agtatgttga aactttttat tgtgagattt gttgcaaaaa ccatctgacc
     5401 ataacgccag ctgcagggga aaggaaggat tatctgcggt ttttgtgatg aaaagcagga
     5461 tgattatatt taacccagta aatacgttcc tgtctttttt gtataaaaaa tgtttattac
     5521 tcgatgtaag gatacattaa ttttacgttt attatgatga atcctctgca aatgagcgaa
     5581 atttgataga acatttatct gctgtacgat ttaatgaact taatgataca gcattacaaa
     5641 aacaatcgaa gtttataaag atgattcctg attgaccaga accctatcct acaatggtga
     5701 ttaattttat tactcgcgat ggagatgatg atatgaatat atcatatatg aatagcaaca
     5761 aaacgacatc tttacctgtt gagctagatg cactaaataa taaagatatc tcttatgcca
     5821 aagatttttc ttatgtcaaa gatttctttt tatacataga aacacagtta aaaattgcaa
     5881 aagatttttg tagacttgga gaagaagtat caagttctat tgcaagtaaa gtttttcacg
     5941 catttattga tttagttaac aaaatcaggg gtaagaaaga ctttatgtat atttgcacgc
     6001 tttgctgttt tgccgaggag gttaaaggtg attattctca ttaca
//
LOCUS       DAJVUJ010000208         6002 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.208, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000208 DAJVUJ010000000
VERSION     DAJVUJ010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6002
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.208"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            1..933
                     /locus_tag="OQQ48_003862"
                     /pseudo
     CDS             1..933
                     /locus_tag="OQQ48_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417591.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1321..1697)
                     /gene="rnpB"
                     /locus_tag="OQQ48_003863"
     ncRNA           complement(1321..1697)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="OQQ48_003863"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(1730..2875)
                     /gene="garK"
                     /locus_tag="OQQ48_003864"
     CDS             complement(1730..2875)
                     /gene="garK"
                     /locus_tag="OQQ48_003864"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HCS1954450.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYAEVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(2972..3862)
                     /gene="garR"
                     /locus_tag="OQQ48_003865"
     CDS             complement(2972..3862)
                     /gene="garR"
                     /locus_tag="OQQ48_003865"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HCS1954451.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(3892..4662)
                     /gene="garL"
                     /locus_tag="OQQ48_003866"
     CDS             complement(3892..4662)
                     /gene="garL"
                     /locus_tag="OQQ48_003866"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HCS1954452.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(4678..5976)
                     /gene="garP"
                     /locus_tag="OQQ48_003867"
     CDS             complement(4678..5976)
                     /gene="garP"
                     /locus_tag="OQQ48_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005155249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HCS1954453.1"
                     /translation="MHTRYLILLIIFIVTAVNYADCATLSIAGTEVAKELQLSTVSMG
                     YIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGFVDMFPLAWAGIS
                     MFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFSLALFSPLLGWLT
                     FAWGWEHVFTVMGVIGFVLTALWIKLIYNPTDHPRMSAEELKFISENGAVVDMDHKKP
                     GSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIYLVQEKGMSILKV
                     GLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLLASTIILCNYTNN
                     TTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGNVASIVTPLVIGY
                     LVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
BASE COUNT         1595 a         1610 c         1349 g         1448 t
ORIGIN      
        1 gactcgtttc ttcgtctgga ttttttaggt tataacgccc aaattattca acgatcggac
       61 aatacctgcg aacttaccat taatgaaccg ttaaaaaacc aggaaatatc tacaggcaat
      121 atcaatgcta attgtccatt aaaagatatt tacaatgaaa tcaggaggtt gaacgcaacc
      181 tttagttgtg ggactggaga tatagttgat ctatcctctc tggacttacg taatgtcgat
      241 ttagattatt atgatttcac agataaacat atggctaata ctattctaaa tccttttaaa
      301 ttggattcaa caaattttac taatgccaac atgtttcagg ttaattttgt tagttcaaca
      361 caaaacgcca caatctcctg ggattattta ctaaaaataa cgcctgtttt aataagtatt
      421 agcgatatgt attctgaaga aaaaatcaag tttgtcgaaa gttgtttaaa tgagcttgga
      481 gacattaccg aagaacaatt aaaaattatg agatttgcaa ttataaaatc tataccaagg
      541 gcaactctta cagataaatt agaaaatgaa ttaacaaaag aaatatataa aagctcatcg
      601 aaaatcaaca attgcttgaa cagaattaaa ttaccagaga tgaaagaatt ctcatcaaga
      661 aaaaacatat gattacatcg atataatcat tgaagattat gaaaatatca aagaaaatgc
      721 ttatctggtc gtcccccaaa ttaattatac tatggattta aacatagaag actccagctc
      781 agaagagtta ataccctcga gaaagacgaa aattcttcgg acaatggctt tgaagtcgaa
      841 gaatataacg catatgaagc atataactca gagaatctat attttaccag agaggaatat
      901 acgtatgatt acgacctttc aaatgcaata tagacttaat aacttcttac aaaatctata
      961 attaataccg tagatattca ttcatcttac cctgtgcacc aaaagcaaaa actcactgac
     1021 gcgggtttga aactgatggc aaccgcaaaa atgcctgatg cgctacgctt atcaggccta
     1081 cgccatctct gcaatatatt gaatttgcgt gcttttgtag gcaggataag gcgttcacgc
     1141 cgcatccggc atgaataaag cgcactcaac aatctgaaac ccgccggaac ggtttattac
     1201 gtacatcagg tgaaactgac cgataagccg ctttcttttg ggtatagtgt cgtggacagt
     1261 cattcatctt tctgcccctc caaaagcaaa aacccgccga agcgggtttt tacgtaaatc
     1321 aggtgaaact gaccgataag ccgggttctg tcgtggacag tcattcatct aggccagcaa
     1381 tcgctcactg gctcaagcag cctacccggg ttcagtacgg gccgtacctt atgaacccct
     1441 atttggcctt gctccgggtg gagtttaccg tgccacggac tgttaccagc cgcgcggtgc
     1501 gctcttaccg caccctttca cccttacctg atcccgcttg cgcgggccat cggcggtttg
     1561 ctctctgttg cactggtcgt gggtttcccc cccaggcgtt acctggcacc ctgccctatg
     1621 gagcccggac tttcctcccc tccgcccgtc tcccccgaag aggacgacga cgaagcggcg
     1681 actgtctggt cagcttcggc gcgcagtata gagggtttgc gcgcccttgt caccccgcgt
     1741 tgcgcattcc aatcgccagt gtcgcggcga tattacgtga agcacggcag atattgtcat
     1801 aagccccgcg gaatgcttcg tccaacgtac ctatgctggt caatacgctg aagaccgcat
     1861 caatgccatg ctgatgtaca acgccaacat catcggtcag gctacccgca atgccaatca
     1921 ccggtttatg gtacttcttc gccacgtttg cgacaccaat cggtaccttg ccgtgaatac
     1981 tctggctgtc aatacgccct tcaccggtga tcaccagcgt acaatcgtga atatgttctt
     2041 ccagattcag cgccgtagtg acgatttcaa taccactttt cagttccgca ccaagaaacg
     2101 ccatcagcgc cgcgcccata ccgcccgcag ctcctgcgcc ggggacatct ttcacatcaa
     2161 catgcagcgc ttttttaatg acctcggcat agtgagagag gttattgtcc agctcaacaa
     2221 tcatcgcttc actggctccc ttttgtgggc caaagatgcg cgatgcgccg ttatcgccca
     2281 ccagcggatt ggtgacatca caagcgacgc gaatgacgca atcttttaag cgcggatcga
     2341 ggccggaaat atcaatatca ttcagagtat taagactacc gccgccaaaa ccaatttcat
     2401 tgccgttggc gtcgcataat ttcgccccca gcgcctgtac catgcctgcg ccgccatcat
     2461 ttgtagcgct gccgccaatg ccgataataa tgtttgtcgc accgctctcc agcgcctgca
     2521 ggattaactc gcctgtgccg cgtgaagtgg tcacgagtgg atcgcgtttt tccgcaggta
     2581 ccagctccag cccactggcc gccgccattt caataaacgc ggttttgcca tcgccggaga
     2641 tcccccaact ggcattcact ttctcgccca acggccctgt aacccaggcg tgacgttcgg
     2701 aaccctgggt ggctgcaatc atcgcttcca ccgttccttc gccaccgtcg gcaaccggaa
     2761 cagaaacgta ctgtgcatca ggaaaaattt cccgaaatcc tttttctatc gcctgcgcaa
     2821 cctcgctggc agataaactt tctttataag agtctggggc gattacgatt ttcataccta
     2881 tgcctgttac cacatgacgc cggagggcgt ttctcttatt cggcgtggat tccaggcccg
     2941 gattgcaata cgccatccgg gcacgacgtc attaacgagt aacttcgact ttcgccagtt
     3001 tttcgtagta gcacgccaga gcgctatgat ccgccgttcc taaaccatct gcacgcagtg
     3061 cctgcatcat ctccataacc gcagctgtga gcggcagttg cgcgccgacg ccgtgagaag
     3121 tatccagcgc attcgccaga tccttaatat gcagatcaat gcggaagccc ggcttgaagt
     3181 tgcggtccat caccatcggc gctttggcat ccagcacggt actgcccgcc agcccaccgc
     3241 gaattgcctg ataaaccagg tctgggttaa cgcccgcttt agttgccagc gttaacgctt
     3301 ctgacatcgc agcaatattc agcgccacaa tgacctgatt tgccagtttg gtgacgttac
     3361 ctgcaccgat ttccccggta tgcaccacgg aacccgccat cgctttcatc aaatcatagt
     3421 atttgtcgaa aatagccttg tcgccgccca ccatcactga cagcgtaccg tcgatggctt
     3481 tcggttcacc gccgctcacc ggagcatcca gcatatcaat gcctttcgct ttcagcgctt
     3541 cgctgatttc acggcttgcc agcggtgcga tagaactcat atcgatcaat accgtacctg
     3601 gcttcgcgcc ttcaataatg ccattctcac ccagcgccac ctctttcaca tgaggggagt
     3661 ttggcagcat ggttatgatg acgtcgcact gttcagcgat cgctttagcc gtagacgctg
     3721 tttctgcacc tgcagcaatc acgtcagcaa tagcttctgg gttacggtca gcaaccacca
     3781 gcgagtaacc tgctttcaga aggtttttac tcattggttt acccataatc cccaggccaa
     3841 taaaaccaac tttcatagtc atatcaatca tctctcttgt tgcggtggtg gttatttttt
     3901 aaaggtatca gccagtttct gagtggcaga gcggaagacg ccaagatcgc tgccgacagc
     3961 cacaaacgtc gcgccccatt ccagataacg acgcgcatcg gcttcgaccg gcgcgagaat
     4021 accgctgggt ttgccgtgcg cactggcacg gttaaaaata tgctgaattg ctttttgtac
     4081 atccggatgc gaggcattgc cgagatggcc caatgccgca gccagatcgc tggggccgac
     4141 gaagataccg tctacacctt cggtagcggc aatagcatcg acgttatcta cgccctgctg
     4201 actttctatc tggaccagaa tagtgatgtt cttgttcgac tgagcgaaat aatccgccac
     4261 ggtgccaaac atattggcgc ggtgagaaac ggagacgccg cgaatgcctt ccggcgggta
     4321 acgagttgat gccaccgcct gctctgcttc ctcttttgtt tctacaaaag gaatcaggaa
     4381 attatagaaa ccgatatcca gaagacgctt aataattacc ggctcgttgg tcggcactcg
     4441 cactactggc gcgctggcgc tgcctttcag ggccattaac tgcggaataa acgtggaaat
     4501 atcgtttggc gcatgttcgc catccagcac cagccagtca aacccagcca aaccaagaac
     4561 ttcagtgcta atcgggttag agagtgctga ccagcaacca atttgtacct gtttcgcagc
     4621 cagtgcggct ttgaatttat tcgggaaaac atcgttattc atcgcttata cctttgctta
     4681 tttctgcaat tccatacgtt taatgtcgcc aactacgaag aggtagcaga ccatcgccat
     4741 cagcgctgaa catcccacga aaaccagtgc tgcattgaag gagtgcagtt cacttaccag
     4801 gtagccaatc accagtggag tgacaatgga ggcaacattg ccaaagacgt taaagacgcc
     4861 gccacagagg ccaacaatct ctttcggcgc ggtgtcagaa atcaccggcc agcccagagc
     4921 accaaatcct ttgccaaaaa aagccagcgc catcagcatg accaccagcg tggtgttgtt
     4981 ggtgtagtta cataagatga tggtggaagc cagcaacatt cccagcacaa tcggtagctt
     5041 acgtgccagg gtcagggata aaccgcgttt gatcagataa tccgagaaga cacctcccag
     5101 cacgccgccc gcaaaaccac acagtgccgg aatcgaggcg accagaccca ctttcagaat
     5161 cgacatgcct ttttcctgca ccagataaat cgggaaccag gtgaggaaga accaggtgat
     5221 ggtgttgata aaatattgtc cgaaaaatac gcccaacatc atgcggttag agagcaattg
     5281 cttgatgtaa tgcagtttgg gaccgcttgc tgccgcgctg cccggctttt tgtggtccat
     5341 atcgaccacc gcgccatttt cagagataaa cttcagctct tccgcagaca tacgtgggtg
     5401 atctgtcggg ttataaatca acttgatcca cagcgccgtc agcacaaaac caatcacccc
     5461 cataacggtg aagacgtgct cccagcccca ggcgaaagtc agccagccaa gcagaggcga
     5521 aaagagcgcc agcgagaaat attgcgccga gttaaagatg gcggaggcag taccacgttc
     5581 tttcgtcggg aaccaggcgg cgacaatacg ggcgttcgcc gggaatgatg gcgcttccga
     5641 gaagccgagc ataaagcgca taaagaacat agagatccct gcccaggcca gcgggaacat
     5701 atcaacaaag ccttgcagga aggtgaatag cgaccagaaa aagaggctgt aggtgtaaac
     5761 ttttttcgag ccaaacttat caagcagcca gccgccggga atttgcatca gcaggtaggc
     5821 ccagccaaaa gcggagaaga tgtaacccat cgaaaccgta cttaactgca actcttttgc
     5881 cacttcggta ccagcaatag acagcgttgc acaatcggcg tagttaacgg cggtaacaat
     5941 aaaaataatc agtaatatta aatagcgggt atgcacgcct ttctttttta cgtcaacggt
     6001 gt
//
LOCUS       DAJVUJ010000209         5816 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.209, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000209 DAJVUJ010000000
VERSION     DAJVUJ010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5816
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.209"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..857
                     /gene="pdeC"
                     /locus_tag="OQQ48_003868"
     CDS             <1..857
                     /gene="pdeC"
                     /locus_tag="OQQ48_003868"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HCS1954454.1"
                     /translation="FALPAGILGSLVLLLLWLRIRQNYLSPKRKLQRALEKHQLCLYY
                     QPIIDIKTEKCIGAEALLRWPSEQEQVMNPAEFIPLAEKEGMIEQVTDYVIDNVFRDL
                     GDYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKFEVTEHAFLDVEK
                     MTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVETLTTHKTSHLIA
                     EHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMPPQVFMQWMEQLP
                     ARELTRGQ"
     gene            complement(860..1183)
                     /gene="soxS"
                     /locus_tag="OQQ48_003869"
     CDS             complement(860..1183)
                     /gene="soxS"
                     /locus_tag="OQQ48_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HCS1954455.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            1269..1733
                     /gene="soxR"
                     /locus_tag="OQQ48_003870"
     CDS             1269..1733
                     /gene="soxR"
                     /locus_tag="OQQ48_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HCS1954456.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYRRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            2279..3628
                     /gene="ghxP"
                     /locus_tag="OQQ48_003871"
                     /gene_synonym="yjcD"
     CDS             2279..3628
                     /gene="ghxP"
                     /locus_tag="OQQ48_003871"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HCS1954457.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFGMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            3780..5429
                     /locus_tag="OQQ48_003872"
     CDS             3780..5429
                     /locus_tag="OQQ48_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HCS1954458.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(5583..>5816)
                     /locus_tag="OQQ48_003873"
     CDS             complement(5583..>5816)
                     /locus_tag="OQQ48_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCS1954459.1"
                     /translation="MFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVTLQKFF
                     EEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
BASE COUNT         1257 a         1376 c         1603 g         1580 t
ORIGIN      
        1 tttttgcgtt gcccgccggt attttgggga gtcttgttct gctattactc tggctacgta
       61 ttcgacaaaa ctatttgtct cccaaacgca aattgcaacg cgctctagaa aaacatcaac
      121 tttgtcttta ttaccagcca ataatcgata tcaaaacaga aaaatgtatc ggcgctgaag
      181 cgttgttacg ttggcctagt gagcaggagc aagtaatgaa tccggcagag tttattccgc
      241 tggcagaaaa ggaggggatg atagaacagg taactgatta tgttattgat aatgtcttcc
      301 gcgatctggg cgattacctg gcaacacatg cagatcgcta tgtttctatt aacctgtcgg
      361 cctccgattt tcatacgtca cggttgatag cgcgaatcaa tcagaaaaca gagcaatacg
      421 cggtgcgtcc gcagcaaatt aaatttgaag tgactgagca tgcatttctt gatgtcgaaa
      481 aaatgacgcc gattattctg gctttccgcc aggctggtta cgaagtggca attgatgatt
      541 ttggtattgg ctactctaac ttgcataacc ttaaatcatt gaatgtcgat attttgaaaa
      601 tcgataaatc gtttgttgaa acgctgacca cccacaaaac cagtcatttg attgcggaac
      661 acatcatcga gctggcgcac agcctggggt taaaaacgat cgctgaaggc gtcgaaactg
      721 aggagcaggt taactggctg cgcaaacgcg gcgtgcgcta ttgccaggga tggttttttg
      781 cgaaggcgat gccgccgcag gtgtttatgc aatggatgga gcaattaccc gcgcgggagt
      841 taacgcgcgg gcaataaaac tacaggcggt ggcgataatc gctgggagtg cgatcaaact
      901 gccgacggaa aacgcgggag aaggtctgct gcgagacata acccaggtcc attgcgatat
      961 caaaaatcgg acgctcggtg gtgcgcaact caacggcggc cagtaacagg cgacgttggc
     1021 gaatgtaatc gccaagcgtc tgatgcgtca ccgtgcggaa cattcgttgc aagtaccact
     1081 ttgaatagcc tgattttttt gcgactacat caatgttaag cggctggtca atatgctcgt
     1141 caatccatgc gataagatcc tgaataattt tctgatggga cataaatctg cctcttttca
     1201 gtgttcagtt cgttaattca tctgttgggg agtataattc ctcaagttaa cttgaggtaa
     1261 agcgatttat ggaaaagaaa ttaccccgca ttaaagcgct gctaaccccc ggcgaagtgg
     1321 cgaaacgcag cggtgtggcg gtatcggcgc tgcatttcta tgaaagtaaa gggttgatta
     1381 ccagtatccg taacagcggc aatcagcggc gatatagacg tgatgtgttg cgatatgttg
     1441 caattatcaa aattgctcag cgtattggca ttccgctggc gaccattggt gaagcgtttg
     1501 gcgtgttgcc agaagggcat acgttaagtg cgaaagagtg gaaacagctt tcgtcccaat
     1561 ggcgagaaga gttggatcgg cgcattcata ccttagtggc gctgcgtgac gaactggacg
     1621 gatgtattgg ttgtggctgc ctttcgcgca gtgattgccc gttgcgtaac ccgggcgacc
     1681 gcttaggaga agaaggtacc ggcgcacgct tgctggaaga tgaacaaaac taaagcgcca
     1741 caagggcgct ttagtttgtt ttccggtctt tgtctttctc tctatcccgc tggtacacag
     1801 gagggtttcc cccgacgtca acacacctca ttcgagcacg tggtggaggt tccggttggt
     1861 gttgatgctt taattgtatg tcaccgacgt ttcttcgcca gtgtaaaagt atacttttta
     1921 accgcaatat ttttgtcatc tcagacgatt ttttatcgca atcctgaacg gtatacggct
     1981 cgataacgct gcaatcttgc gcaccgacga taacgtttgc gcatcaattg cctgtttttt
     2041 catcgtcaag acaataaaag agaaaaaagc agcaaacttc ggttgaaaaa gccgctatga
     2101 tcgccggata atcgtttgct ttttttacca cccgttttgt atgcgcggag ctaaacgttt
     2161 gcttttttgc gacgcagtaa attgtcgcaa acctggagca ggaagataac gtttcgctgg
     2221 caggggattg tccgccacgc atcttgacga aaattaaact ctcaggggat gttttcttat
     2281 gtctacgcca tcagcgcgta ccggcggttc actcgacgcc tggtttaaaa tttcacaacg
     2341 tggaagcact gtccgtcagg aagtggttgc cgggttaaca acgtttctgg cgatggtcta
     2401 ctcggtcatc gtcgttccag gtatgttggg taaagcaggc ttcccgcctg cggcagtttt
     2461 cgttgcaacc tgtctggttg ccggactcgg ttctatcgtg atgggtctgt gggctaatct
     2521 gccgttggcg attggttgcg ccatctccct gacggcgttt accgcattca gcctggtgct
     2581 ggggcaacat attagcgtac ctgtcgcgct gggtgccgtg ttcctgatgg gtgtgctgtt
     2641 tacggtaatt tctgccacgg gtatccgtag ctggattttg cgcaacttgc cgcacggtgt
     2701 ggcgcacggc acggggatcg gtatcggtct gttcctgctg ctcattgccg ctaatggtgt
     2761 tggtctggtg attaaaaacc cgcttgatgg tctgcccgtt gcgctgggtg atttcgcgac
     2821 cttcccggtg attatgtcac tggtaggtct ggcggtgatc atcggcctgg aaaaactgaa
     2881 agtccctggt ggcattctgc tgaccattat cggtatctca attgtcggtt tgatcttcga
     2941 tcctaacgtc catttctccg gcgttttcgg catgccttca ttgagcgatg aaaacggcaa
     3001 ttcactgatt ggcagcctgg acattatggg cgcgctgaat cctgtagtcc tgccaagcgt
     3061 tctggcgctg gtgatgacgg cagtatttga tgccaccgga actatccgtg ccgtcgccgg
     3121 ccaggcgaac ctgctggata aagatgggca gatcatcgac ggtgggaaag cactgaccac
     3181 tgactccatg agcagcgttt tctctggcct ggtgggtgct gctccggcag cggtatacat
     3241 cgagtctgcg gcgggtacgg cggcgggcgg taaaaccggt ttgacggcta tcaccgttgg
     3301 cgtgctgttc ctgctgattc tgttcctctc tccgctctct tacctcgttc cggggtatgc
     3361 aacggctccg gcgctgatgt acgttggcct gctgatgctg agcaacgtgg cgaaaatcga
     3421 ctttgctgat tttgttgatg cgatggcggg tctggttacg gcggtattca tcgtgctgac
     3481 ctgtaacatc gtaacaggca tcatgatcgg cttcgcgact ctggtgattg gtcgtctggt
     3541 ttccggcgaa tggcgcaagt tgaacatcgg tacggtcgtt atcgccgtgg cgctggtgac
     3601 cttctatgcg ggtggctggg ctatctaatc tttcatcgga ttctgaaaac gggtggcaat
     3661 ggctgcccgt ttttattttc tccgcacatt gtgtgagctt ttgcgatatt ctgaaaaaat
     3721 gagaattcag gcataacgcc tttctcagat cataaaaaaa catcgcaaac agggaacgca
     3781 tggaaatctt cttcaccata ctgataatga ccctcgtggt ctcgctgtcc ggggtggtca
     3841 ctcgtgtcat gccctttcag atcccgcttc cgcttatgca aatcgccatc ggtgcgctac
     3901 tggcgtggcc gacgtttggt ttgcatgtgg agtttgatcc tgaactcttt ttagtcttgt
     3961 ttatcccgcc gttgctgttc gctgatggct ggaaaacgcc gacccgtgaa tttctcgaac
     4021 atggtcgaga gattttcggc ctcgcgctgg cgctggtggt ggtcaccgtg gtcggcattg
     4081 gcttccttat ttactgggtg gtgccgggca ttccgctgat ccccgccttt gcgctggcgg
     4141 cggtactttc tccgaccgat gctgtggcgc tctccggaat tgttggcgaa gggcgcatcc
     4201 cgaaaaaaat catgggcatt ttgcagggcg aagcgttgat gaatgacgcc tccggcctgg
     4261 tgtcgttgaa gtttgccgtg gcagtggcga tggggacgat gatcttcacc gtcggcggtg
     4321 cgacggtcga atttatgaaa gtagccattg gtggtattct tgccggtttt gtggtgagct
     4381 ggctgtatgg tcgctcgctg cgattcctca gccgctgggg cggtgatgaa cccgcgacgc
     4441 agatcgttct gctgttcttg ctgccattcg cttcttatct gattgccgaa catattggcg
     4501 tttccggcat cctcgctgcg gttgccgccg ggatgaccat cacccgctcc ggtgtgatgc
     4561 gccgtgcgcc gctggcaatg cgcctgcgcg caaacagcac ctgggcgatg ctggaatttg
     4621 tctttaacgg catggtgttc ctgctgttag gtctgcagct gccgggtatt ctggagacgt
     4681 cgctgatggc ggcagaaatc gaccctaacg tcgaaatctg gatgctgttt accgatatta
     4741 ttctgatata tgcggcgctg atgctggtcc gtttcggctg gctgtggacg atgaaaaagt
     4801 tcagcaaccg cttcctgaag aagaagccga tggagtttgg ttcgtggacc acacgagaaa
     4861 tcctgatcgc gtctttcgcc ggggtgcgtg gggcgatcac tctggccggt gtgctctcta
     4921 tcccgctgct cttgccggat ggtaacgtct tcccggcgcg ctatgagctg gtgttcctgg
     4981 cggctggcgt cattctcttc tcgctgtttg tcggtgtggt gatgttgcct attctgctac
     5041 aacacattga agtcgcggat cattcgcaac aattgaaaga ggaacgtatt gcgcgagcgg
     5101 caacggcaga agtggcgatt gtggcgatcc agaaaatgga ggagcgtctg gcggcggata
     5161 ccgaagagaa tatcgataac cagctgctta cggaggtcag ttctcgcgtc attggtaacc
     5221 tgcgtcgtcg cgccgatgga cgtaacgacg ttgaaagttc cgtgcaggaa gagaaccttg
     5281 agcgtcgctt ccgtctggcg gcattgcgtt ctgaacgtgc tgaactgtac cacctgcgcg
     5341 ccacgcggga gatcagcaac gaaacgctgc aaaaattact gcacgatctc gatttgcttg
     5401 aagcgttgct aattgaggaa aatcagtaag ccgaaagcga cgtaaccagg cccgaggtaa
     5461 tctgaaaaag gtgcaatctt ttgcaccttt aacaggttag ctcgggctaa cattcatgat
     5521 tctaaaacaa ataagttatc tctcttgtaa agtcaattca ttggctgttc ttcgacttaa
     5581 cctcaatctg cgtgttgcat aatatctgca gtaatcttca tagcgactgc attaatatta
     5641 tcctcagttt gtgacatgcc agtatgagag gtacaatctt cttcaaagaa tttctgtagt
     5701 gttaccaggg tatcactatc aatggggata ttttcctctc cacggagtat ggaaatgccc
     5761 attccctgga cgctagcctc atcaaaatta acccgataga gacgggtatt aaacat
//
LOCUS       DAJVUJ010000210         5792 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.210, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000210 DAJVUJ010000000
VERSION     DAJVUJ010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5792
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.210"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(135..2054)
                     /gene="dhaR"
                     /locus_tag="OQQ48_003874"
     CDS             complement(135..2054)
                     /gene="dhaR"
                     /locus_tag="OQQ48_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HCS1954460.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWSVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     HDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLEATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            2282..3391
                     /gene="dhaK"
                     /locus_tag="OQQ48_003875"
     CDS             2282..3391
                     /gene="dhaK"
                     /locus_tag="OQQ48_003875"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HCS1954461.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECAMQVDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRSFSSLDQTVDEMFDTLLGNGSYHRTLRFWDYQQGSWQEEQQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWDKLGESNVTEQNSNC"
     gene            3363..3995
                     /gene="dhaL"
                     /locus_tag="OQQ48_003876"
     CDS             3363..3995
                     /gene="dhaL"
                     /locus_tag="OQQ48_003876"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HCS1954462.1"
                     /translation="MSLSRTQIVNWLTRCGDIFSTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            4006..5424
                     /gene="dhaM"
                     /locus_tag="OQQ48_003877"
     CDS             4006..5424
                     /gene="dhaM"
                     /locus_tag="OQQ48_003877"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HCS1954463.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPKIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPAYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVPPVSGKAFYYQPVLYTVQAKSTL
                     TVEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDYIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
BASE COUNT         1459 a         1475 c         1427 g         1431 t
ORIGIN      
        1 agcaagtcaa cggaggatat cgcttcagct tttaagtgcg tgatagacaa gcccttaaac
       61 gatgctgaaa aatgttagcg tgcgccggaa tgaaatatag atcactctga tgtattagaa
      121 atcgtttctg tctttcatac ccggcgctta aactgccctg catcaatgcc atgttgcttc
      181 attttccgcc acagcgtagt gcggccgatt ccaagtaaag ccgacatttc ctgaatgcga
      241 ccgcctgtga cctgggctgc gttaataatt gcctcttttt caacttccgc aaatgacaga
      301 ctggtggaaa ggcgggtggc gctgacatca tctgttgcct gctcggtaaa cagatgttcc
      361 ggcaaatcac tgacgcgaat gcgcccatta tcactactca gagcaagatt ctcgatgacg
      421 ctgtaaagtt caaaatcgtt gcctggccat gcacaagaaa ccaggcgagc gagggcatcg
      481 tcatcaattt tcagccgcgt agagaagcgt ttttcaagac tgcgtaattt gttattcacc
      541 agcgccggaa tgctgccacg ccgcatacgc agaggcggga tggtaatttc aaatgcatgc
      601 agcgcgtaat acagctggcg actaaaacga ttttgttcca ccagcattgc gaggtccgcg
      661 gtcgttgttg caatcacttt gacatcaatt ggtattaaac gccgcgcatc cagtcgcgtg
      721 ataacaccct gcttgataac ctgaagcaaa gcagactgta actccaccgc cagatattca
      781 atcttttcaa gaaacagcgt gccgccgtgc gccagttcca gccgactcag acggccattt
      841 tcattgtccg tacgatcgcc accaataaat tcttccgcca gtgccgcatc accatataac
      901 tcacaattga cggcgatata aggacctgca gcacgctcgc tttcattatg aattgcctga
      961 cttagcagtg ccttgcccac gccctcttct ccacaaagca ggacaggaaa gctactgcgc
     1021 gccgcctggc gaccaaaatg aatcaagcgg cgggtttgcg gatcgtcctg tggcatatga
     1081 gcaaaggtat ggctgacttt tcctaattga ctggtcatca actgccgcat ctgttccaca
     1141 ggatggagca acaaaataaa gctggttccc tgcgtttcta ttatcggttt aagggttatc
     1201 accgcatcaa taaactgatg ctgactttca aaggttgctt ctacgtgttt gagcggatgt
     1261 gcctgtttta ttgcttgttg caatacggcg ggtaacgtta agagttcagt gattgcccgt
     1321 ccctgacttg ccgtcgcttc aaggcgcaag acccgcgccg cctgggcatt gataaattgc
     1381 aaattaccct gctcgtccca gctaatcacg ccatcatcca tactttctaa cagggcatta
     1441 agttgattta aatgacggtt agtttcagcg agcaaactgt ccgtcagcag taaatttccg
     1501 acctcgcggg cgattgccaa cgtcaacggc aaatcagctg cggtagtttg ctcaaccgga
     1561 cacgccagcg ctattgttcc cgtcaatcgg cccttgctgt caaacaacgg cgttgcacaa
     1621 aaggcccagt tccagagtgc ctgtttgaaa tgttgatcgg ccatcgtttt cacggcctga
     1681 ccagagatag ccgctaacga tagcgcacaa gtaccaataa ttccctcggc gcaatacgtg
     1741 ccgtcatgga atccaagtgc acttagctgc tgcaaggttt gcggatcgcc attacggctg
     1801 agaatgcagg cggtttcatc gaggataaac agcgcacact ctcgcggtgc catatattcc
     1861 caggcatctt ccagcgcagc ctgcccgagc gtcagcatcg ctttcttacg ccgataaata
     1921 gaagcaaatg tcacgccctg ggcctgatgt ggtacgctcc atgtctcccg tttcatcagc
     1981 ttattgcatc gctcccagga ggttgcaatt aagggagata tgccccgacc atcgttgtta
     2041 aaagcgccac tcatatcccg catcccttat gacaaaaggt gttccggctc gcaattttaa
     2101 gtattaatac acgtaaaagt ttcaatatgg aacattcatt aaagcatatg tttcactatg
     2161 aaacataatt tgggctaatt ttcttttccc ttgcctgatg cacaatggat tcatcggcag
     2221 tcagtggtcg ccgtgtcgtt gaacatcatc catgccctac cgtaattgct ggagcaaaat
     2281 aatgaaaaaa ttgatcaatg atgtgcaaga cgtactggac gaacaactgg caggactggc
     2341 gaaagcgcat ccatcgctga cattgcatca ggatccggtg tatgtcaccc gagctgatgc
     2401 ccctgttgca ggaaaagtcg ccctgctgtc gggtggcggc agcggacacg agccgatgca
     2461 ctgtgggtat atcggtcagg ggatgctttc gggggcctgt ccgggcgaaa ttttcacctc
     2521 accgacgccc gataaaatct ttgaatgcgc catgcaagtt gatggcggcg aaggtgtact
     2581 gttgattatc aaaaattaca ccggcgatat tcttaacttt gaaacagcga ccgagttact
     2641 gcacgatagc ggcgtaaaag tgaccactgt ggtcattgat gacgacgttg cggtaaaaga
     2701 cagtctttat actgccgggc gacgcggcgt tgccaacacc gtattaattg aaaaactcgt
     2761 aggcgcagcg gcggagcgtg gcgactcact ggacgcctgt gcggaactgg ggcgtaagtt
     2821 gaataatcaa ggccactcaa taggtatcgc tctcggtgcc tgtaccgttc ctgccgcggg
     2881 caaaccttct tttaccctgg cggataatga gatggagttt ggcgtcggca ttcatggtga
     2941 gccgggtatt gaccgccgct ccttctcttc ccttgatcaa accgtcgatg aaatgttcga
     3001 caccctgctg ggaaatggct cataccatcg cactttacgt ttctgggatt atcaacaagg
     3061 cagttggcag gaagaacaac aaaccaaaca accgctccag tctggcgatc gggtgattgc
     3121 gctggttaac aatcttggcg caactccgct ttctgagctg tacggcgtct ataaccgcct
     3181 gaccacacgt tgccagcaag cgggattgac tatcgaacgt aatttaattg gcgcgtactg
     3241 tacctcactg gatatgaccg gtttctcaat caccttactg aaagttgatg acgaaacgct
     3301 ggcactctgg gacgccccgg tccacacccc ggcccttaac tgggataaat taggagaaag
     3361 caatgtcact gagcagaact caaattgtta actggctcac tcgttgtggc gatattttca
     3421 gcaccgagag cgagtatctt accggactgg atcgcgaaat tggcgacgct gaccacgggc
     3481 taaatatgaa ccgaggcttt agcaaagtgg tggaaaaact ccctgctatc gcagataaag
     3541 atatcggttt cattctcaag aataccggta tgacgctgct ttccagcgtc ggtggtgcca
     3601 gtggtccgct gttcggtacc ttctttatcc gcgccgcaca ggcgacccag gcacggcaaa
     3661 gcctgacact ggaagagctt tatcagatgt tccgcgatgg cgcggacggc gtaatcagtc
     3721 gcgggaaagc cgaacctggc gataaaacca tgtgtgatgt gtgggtgccg gtggtggaat
     3781 cgttacgtca gtccagcgag caaaatctct ctgttccggt ggcgctcgaa gctgccagta
     3841 gcatcgccga atccgctgca caaagtacga ttacgatgca agcccgcaaa ggccgcgcca
     3901 gttatctcgg tgaacgcagt attggtcacc aggatcccgg cgcgacctcg gtgatgttta
     3961 tgatgcaaat gttggcgtta gccgcaaaag agtaaggaat tggtgatggt aaacctggtc
     4021 atagtttcac atagcagccg actgggagaa ggtgtcggtg aattagcccg tcagatgtta
     4081 atgagtgata gttgtaaaat cgccattgcc gcgggaattg acgatccaca aaatcctatt
     4141 ggtaccgatg ccgtcaaagt gatggaggcc atcgaatctg ttgctgatgc cgaccatgtg
     4201 ctggtcatga tggatatggg tagcgcatta ttgagtgctg aaactgcgct ggaattgctg
     4261 gctcccaaga tcgccgcaaa agtacgtttg tgtgctgcgc cgttggtcga aggtacactg
     4321 gcagcaacgg tcagcgcggc ctcgggagcg gatatcgaca aagttatctt tgacgctatg
     4381 catgcgctgg aagccaaacg tgaacaactg ggtttaccgt cctccgacac tgaaatctct
     4441 gacacatgtc ctgcgtacga tgaagaagcc cgttctctgg cggtggtcat aaaaaaccgt
     4501 aacggcctgc atgtacgtcc ggcctcccgg ctggtttata ccttatcgac atttaatgcc
     4561 gatatgttgc tggaaaaaaa cggcaaatgc gtcacaccag agagtattaa ccagattgcg
     4621 ttactacaag ttcgctataa cgatacgctg cgcctgattg cgaaagggcc agaagctgaa
     4681 gaggcactga tcgctttccg tcagctggct gaagataact ttggtgaaac ggaggaagtc
     4741 gctccaccta ctctgcgtcc cgttccgcct gtttcgggta aagcctttta ttatcaacca
     4801 gttttatata cggtacaggc aaaatcaacc ctgaccgtgg aagaagaaca agatcgatta
     4861 cgccaggcta ttgacttcac gttattagat ctgatgacgt taacagcgaa agcagaagcc
     4921 agcgggcttg actatattgc cgcaatcttt tctggtcacc atacactgtt agatgatccg
     4981 gaactgctgg cggcggcaag cgaactcctt cagcatgaac attgcacggc agaatatgcc
     5041 tggcagcaag ttcttaaaga acttagccag caataccagc aactggatga tgaatatcta
     5101 caagctcgct atattgatgt ggacgatctt ctgcatcgca ccctggtcca cctgacccaa
     5161 acgaaagaag aactcccgca gtttaactcg ccaactattc tactggcgga gaacatttat
     5221 ccttccacag tactgcaact ggatccggcg gttgtaaaag gtatctgcct tagcgccgga
     5281 agtccggtat cccacagcgc cctaatcgcc cgtgaactgg ggattggctg gatttgccag
     5341 cagggtgaga aactgtatgc gatacaacca gaagaaacgc taacgctgga cgttaaaacg
     5401 caacgtttca accgtcaggg ttaagccact gttcggatgg catcgttctg atgtcatccg
     5461 gcaatttact cagcactaat ggtttttaac tacctgttat atttttcacc aatttatgat
     5521 gatttttctt cgctaatcac gaacccaacg ccgctggctg gctaataaac agacgttatt
     5581 tgatcactct atttcgccgt tatttatccc tttaattccc ttttctaaaa tgcctgacag
     5641 ttcgcagaat gagatttcga tcatgcagct agtgcgatcc tgaactaagg ttttctgata
     5701 cttgaatacc gttttattcg gtttcgccaa aggagaatga ttgatgaaat cccccgcacc
     5761 ttctcgcccg caaaaaatgg cgttaattcc ag
//
LOCUS       DAJVUJ010000211         5673 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.211, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000211 DAJVUJ010000000
VERSION     DAJVUJ010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5673
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.211"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            190..2600
                     /locus_tag="OQQ48_003878"
                     /pseudo
     CDS             190..2600
                     /locus_tag="OQQ48_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416104.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase subunit A"
     gene            2661..5084
                     /locus_tag="OQQ48_003879"
     CDS             2661..5084
                     /locus_tag="OQQ48_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase subunit A"
                     /protein_id="HCS1954464.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAEAPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKCGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLITENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMLDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEIIHPLPAYTPGFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            5095..>5673
                     /gene="dmsB"
                     /locus_tag="OQQ48_003880"
     CDS             5095..>5673
                     /gene="dmsB"
                     /locus_tag="OQQ48_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCS1954465.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELGCKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGIWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICIESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTG"
BASE COUNT         1535 a         1440 c         1422 g         1276 t
ORIGIN      
        1 actgaacgcc ggatatgaca catccggcga tttgaacata caacataatc ccaccttatt
       61 actcataccc ttctattgat gtggattaat aattcttaac ccaaaatggg tagacccccc
      121 tctattgtta gcgcgctaaa tattcaatac ataaacattt atataacgat aaagaacagg
      181 gagtgagtta tgtccaaaaa tgaccgaatg gtgggcatca gccgcagaac actcgttaaa
      241 tcaaccgcga taggttctct ggcgctggct gcaggcggtt tttctttacc gttcaccctg
      301 cgcagtgcag cggcaacggt acaacaggcc agcgaaaaag tgatctgggg tgcctgttcc
      361 gtcaactgtg gtagccgctg tgcacttcgt ctacatgtta aagataatga agtgacctgg
      421 gtggaaactg acaataccgg cagcgatgag tacggcaacc atcaggtacg cgcctgtttg
      481 cgcggtcgct ccatccgccg gcgtattaat catcccgatc gcttgaatta cccaatgaaa
      541 cgcgtgggta aacgcggtga aggcaaattc gaacggatta gctgggatga agccctggat
      601 actatcgcca gtagcctgaa aaaaaccgtc gaacaatatg gcaatgaggc tgtatatatt
      661 cagtactctt cggggattgt tggcggcaat atgacccgct cttcgccatc agcctcggcg
      721 gtcaaacgcc tgatgaactg ctacggcggt tcactcaatc agtatggctc ctacagcacc
      781 gcacaaattt cctgtgccat gccctacacc tacggcagta atgatggcaa cagcaccacg
      841 gatattgaaa acagcaagct ggtcgtgatg tttggtaaca acccggcaga aacccgcatg
      901 agcggcggtg gcatcactta tcttcttgaa aaagcgcgcg agaaatcgaa cgccaaaatg
      961 attgttatcg atccgcgata taccgatacg gctgctggtc gtgaagatga atggcttcct
     1021 attcgcccgg gcaccgatgc cgcgctggtt gcgggtattg cctgggtatt gattaacgaa
     1081 aatctcgttg atcaaccttt tctcgataaa tactgcgtcg gctatgacga aaaaacctta
     1141 cccgcagatg caccaaaaaa tggtcactat aaagcctata ttcttggtga aggtgatgat
     1201 aatacagcga aaacgccgca gtgggcttcg caaattaccg gtatcccggt ggaccgtatc
     1261 atcaaactgg cgcgtgaaat tggcacagca aaacccgctt atatctgcca gggctggggg
     1321 ccgcaacgcc aggcaaacgg cgaactgact gcacgcgcta ttgctatgtt gcctattttg
     1381 acgggcaatg tcggcatcag cggcggaaat agtggcgcgc gtgaatcgac ctataccatt
     1441 accatagaac gcctgccggt gctggataat ccggtcaaaa cgtcaatctc ctgcttcagc
     1501 tggacagatg ctatcgatca tggtccgcaa atgacggcaa tccgcgacgg cgtccgcggc
     1561 aaagataaac tggatgtgcc cattaagttc atctggaact acgcgggaaa taccctcgtt
     1621 aatcagcatt ctgacatcaa caaaacgcat gaaattctgc aggacgaatc gaaatgcgaa
     1681 atgattgtgg tcatcgaaaa ctttatgacc tcatcggcaa actgccagac ctgatgaccg
     1741 ttgagcagga agatattatt cctaacgact acgccggtaa catgggatat ctcattttcc
     1801 tccagcctgt caccagcgaa aaattcgaac gcaaaccgat ttactggatc ctgagtgaag
     1861 tcgcgaaacg tcttggacct gacgtctatc aaaagttcac agaaggtcgc acgcaggaac
     1921 aatggttaca acatctgtac gccaaaatgc ttgccaaaga tccggcgtta ccgtcttacg
     1981 acgaactgaa aaaaatgggt atctataagc gtaaagatcc caatggccat tttgtcgcct
     2041 acaaagcatt tcgtgacgac cccgaggcaa atccacttaa aacgccttcc ggtaagattg
     2101 aaatttattc cagcaggctg gcggaaattg cccgtacctg ggaactggaa aaagatgaag
     2161 tgataagccc attgccagtt tatgcctcaa cctttgaagg ctggaactcc cctgaacgta
     2221 gaaccttccc actgcaactg tttggtttcc attacaaatc ccgtactcac tcgacctacg
     2281 gcaatattga tctcctgaag gctgcctgcc gtcaggaggt gtggatcaac cctatagatg
     2341 cgcagaaacg tgggattgcc aacggcgata tggtgcgggt gtttaaccat cgtggcgaag
     2401 ttcggctacc agccaaagta acaccacgta ttctccctgg agttagcgct atgggccagg
     2461 gagcctggca cgaggccaat atgtctggcg ataaaatcga ccatggcggc tgtgtgaata
     2521 cgctaaccac tctgcgccct tcaccactgg cgaagggaaa cccgcagcac actaatctgg
     2581 tcgagatcga aaaaatataa cccacgacaa ccataaattc tggcatgaca ttttgtttga
     2641 aaagcaataa gtgagtaatg atgaaaatcc ataccacaga ggcgctcatg aaggctgaaa
     2701 tcagccgtag aagtctgatg aaaacctccg cacttggcag tcttgcgctg gcaagcagtg
     2761 ctttcactct gccattttcc caaatggtcc gggcggcaga ggctccggta gaagagaaag
     2821 cggtctggag ttcctgcacc gttaactgcg ggagccgctg tctgttacgt ttgcatgtga
     2881 aagatgacac cgtgtactgg gtggagtctg atacgacagg tgacgacgtc tacggtaatc
     2941 atcaggttcg agcgtgttta cgcgggcgct ctatccgccg acggatgaat catcctgata
     3001 ggttgaaata tcccatgaag cgcgtcggca agtgcggtga aggtaaattt gaacggataa
     3061 gttgggacga agccctggat accatcagtg ataatcttcg gcggatcctg aaagattacg
     3121 gcaatgaggc tgtacatgtc ctgtacggaa caggcgtaga tggcggaaac atcaccaact
     3181 caaacgtccc gtaccgtctg atgaactctt gcggtggttt tctcagtcgc tatggcagct
     3241 acagtaccgc acagatcagt gccgcaatga gttatatgtt cggtgccaat gatggcaaca
     3301 gcccggatga tatcgccaat acgaaactgg tcgttatgtt cggaaataac ccggcagaaa
     3361 cgcggatgag cggcggtggt gtcacttact acgtcgagca agcccgcgaa cgttcaaacg
     3421 cacgcatgat cgtcatcgat ccacgttata acgacactgc tgccgggcgt gaagatgaat
     3481 ggctgcctat tcgccctggc accgatggcg cactggcctg tgcgattgcc tgggtactga
     3541 ttactgaaaa catggtcgat cagccatttc tcgacaaata ttgtgttggt tacgatgaaa
     3601 aaacgctgcc cgccaacgca ccacgtaacg cgcattataa agcctatatt ctgggcgaag
     3661 ggcctgacgg catagctaaa acgccggaat gggcagcaaa aatcaccagc atcccggcag
     3721 aaaaaattat ccagttggcg cgagagatcg gttcagcaaa acctgcttat atttgtcagg
     3781 gttgggggcc acaacgacat tccaacggcg agcaaacgtc ccgcgctatt gccatgcttt
     3841 ccgttctcac cggtaacgtt ggcataaacg gcggcaactc aggtgtacgc gaaggtagct
     3901 gggatctggg ggtagaatgg ttcccgatgc ttgagaatcc tgttaaaacg cagatttccg
     3961 tctttacatg gacagatgcc atcgaccatg gtacggaaat gaccgcgacc cgtgatggtg
     4021 ttcgtggaaa agaaaaactg gatgtcccca tcaagttttt atggtgctac gccagtaaca
     4081 cattgatcaa tcaacatggc gacatcaatc acacccatga ggtgcttcag gatgacagca
     4141 agtgcgagat gattgttggc attgaccact tcatgacggc ctcggctaag tattgcgata
     4201 tcctgttgcc cgacctgatg ccaacagagc aagaagacct tatctctcat gaatctgcag
     4261 ggaatatggg ctatgtgatc ctcgcccaac ccgcaacctc agcaaaattt gaacgcaaac
     4321 ccatctactg gatgctgagt gaagtcgcca aacgcttagg accagacgtt tatcaaacct
     4381 ttactgaagg tcgcagtcag catgaatgga tcaaatatct ccatgcgaaa acgaaggaac
     4441 gtaaccctga gatgctcgac tacgaggaga tgaaaacgac cgggatcttt aagaaaaaat
     4501 gcccggaaga acactacgtc gctttccgcg cattccgtga agatccacag gcaaacccgt
     4561 tgaaaacacc ttcggggaaa atcgaaattt attctgaacg actggcgaag attgcagata
     4621 cctgggaatt gaaaaaagat gaaattattc atccccttcc tgcgtatacc ccaggttttg
     4681 atggctggga cgatcccctg cggaaaacct atccactgca gttaacgggc ttccactaca
     4741 aagcgcgtac ccactccagc tacggcaata ttgatgtgtt acagcaggcc tgcccacaag
     4801 aggtgtggat caaccccatt gatgctcagg cacgcggtat ccgtcatggc gataccgtgc
     4861 gggtatttaa caataatgga gaaatgctga ttgccgcaaa agtgactccg cgtattctgc
     4921 ctggcgttac cgccatcggg caaggtgcgt ggcttaaagc ggatatgttt ggtgaccggg
     4981 tcgatcacgg cggcagtatc aatattctga cctctcaccg cccttcaccg ctggcaaagg
     5041 gaaacccgtc gcacagcaat cttgtccaga tcgaaaaggt ttaaggagta gcccatgacc
     5101 acacaatatg gattttttat cgattccagc cgctgtactg gctgtaaaac ctgcgaactg
     5161 gggtgcaaag attttaaaga tcttggcccg gaagtcagtt tccgccgtat ttatgaatat
     5221 gctggcggcg actggcagga agataatggc atctggcacc agaacgtgtt cgcttattat
     5281 ctctccatct cctgtaacca ttgcgatgac cccgcatgta caaaagtctg ccccagcggc
     5341 gcaatgcata agcgtgaaga tggctttgtg gtggttgatg aagatgtttg tatcggctgc
     5401 cgctactgcc acatggcttg tccgtacggc gcaccacagt acaatgctga aaaagggcac
     5461 atgacgaagt gcgatggttg ttattcgcgc gtcgccgagg ggaaacaacc catatgcatc
     5521 gaatcctgcc cgctgcgtgc acttgagttc ggtccaattg aagagctgcg ccagaagcac
     5581 ggcactctgg ctgccgtcgc gccactgccg cgcgcgcatt tcacaaaacc caatatcgtt
     5641 atcaaaccta acgccaacag ccgcccgacc ggc
//
LOCUS       DAJVUJ010000212         5643 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.212, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000212 DAJVUJ010000000
VERSION     DAJVUJ010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5643
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.212"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..3746
                     /gene="sigA"
                     /locus_tag="OQQ48_003881"
     CDS             <1..3746
                     /gene="sigA"
                     /locus_tag="OQQ48_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine protease autotransporter toxin SigA"
                     /protein_id="HCS1954466.1"
                     /translation="IILSSIYGSYGETAFAAMLDINNIWTRDYLDLAQNRGEFRPGAT
                     NVQLMMKDGKIFHFPELPVPDFSAVSNKGATTSIGGAYSVTATHNGTQHHAITTQSWD
                     QTAYKASNRVSSGDFSVHRLNKFVVETTGVTESADFSLSPEDAMKRYGVNYNGKEQII
                     GFRAGAGTTSTILNGKQYLFGQNYNPDLLSASLFNLDWKNKSYIYTNRTPFKNSPIFG
                     DSGSGSYLYDKEQQKWVFHGVTSTVGFISSTNIAWTNYSLFNNILVNNLKKNFTNTMQ
                     LDGKKQELSSIIKDKDLSVSGGGELTLKQDTDLGIGGLIFDKNQTYKVYGKDKSYKGA
                     GIDIDNNTTVEWNVKGVAGDNLHKIGSGTLDVKIAQGNNLKIGNGTVILSAEKAFNKI
                     YMAGGKGTVKINAKDALSESGNGEIYFTRNGGTLDLNGYDQSFQKIAATDAGTTVTNS
                     NVKQSTLSLTNTDAYMYHGNVSGNISINHIINTTQQHNNNANLIFDGSVDIKNDISVR
                     NAQLTLQGHATEHAIFKEGNNNCPIPFLCQKDYSAAIKDQESTVNKRYNTEYKSNNQI
                     ASFSQPDWESRKFNFRKLNLENATLSIGRGANVKGHIEAKNSQIVLGNKTAYIDMFSG
                     RNITGEGFGFRQQLRSGDSAGESSFNGSLSAQNSKITVGDKSTVTMTGALSLINTDLI
                     INKGATVTAQGKMYVDKAIELAGTLTLTGTPTENNKYSPAIYMSDGYNMTEDGATLKA
                     QNYAWVNGNIKSDKKASILFGVDQYKEDNLDKTTHTPLATGLLGGFDTSYTGGIDAPA
                     ASASMYNTLWRVNGQSALQSLKTRDSLLLFSNIENSGFHTVTVNTLDATNTAVIMRAD
                     LSQSVNQSDKLIVKNQLTGSNNSLSVDIQKVGNNNSGLNVDLITAPKGSNKEIFKAST
                     QAIGFSNISPVISTKEDQEHTTWTLTGYKVAENTASSSAAKSYMSGNYKAFLTEVNNL
                     NKRMGDLRDTNGEVGAWARIMSGAGSASGGYSDNYTHVQIGVDKKHELDGLDLFTGLT
                     MTYTDSHASSNAFSGKTKSVGAGLYASAIFDSGAYIDLISKYVHHDNEYSATFAGLGT
                     KDYSSHSLYVGAEAGYRYHVTEDSWIEPQAELVYGAVSGKRFDWQDRGMSVTMKDKDF
                     NPLIGRTGVDVGKSFSGKDWKVTARAGLGYQFDLFANGETVLSDASGEKRIKGEKDGR
                     ILMNVGLNAEIRDNLRFGLEFEKSAFGKYNVDNAINANFRYSF"
     gene            3793..4375
                     /locus_tag="OQQ48_003882"
                     /pseudo
     CDS             3793..4375
                     /locus_tag="OQQ48_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4778..5178)
                     /locus_tag="OQQ48_003883"
                     /pseudo
     CDS             complement(4778..5178)
                     /locus_tag="OQQ48_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            5182..5643
                     /locus_tag="OQQ48_003884"
                     /pseudo
     CDS             5182..5643
                     /locus_tag="OQQ48_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1724 a         1200 c         1305 g         1414 t
ORIGIN      
        1 gtattatttt atcctcaata tatggaagtt atggtgaaac agcatttgca gcaatgctgg
       61 atataaataa tatatggacc cgcgattatc ttgaccttgc tcaaaacaga ggagagttca
      121 gaccgggtgc aacaaatgtt caattaatga tgaaagatgg aaagatattt cattttccag
      181 aactacctgt acctgatttt tctgctgttt ccaacaaagg tgcaacaaca tcaattggag
      241 gtgcgtacag tgttactgcg actcataacg gtacacagca tcatgcaata acaacacagt
      301 catgggatca gacagcatat aaagcaagta acagagtatc atctggcgac ttttcggttc
      361 atcgtctgaa taaattcgtc gtggaaacaa caggggttac ggagagtgcc gacttctcac
      421 tttctcccga agatgcgatg aaaagatatg gcgtaaacta caacggtaag gaacaaataa
      481 ttggcttcag agcaggtgcc ggaacaacct caacgatatt aaacggcaaa caatatctgt
      541 ttggacaaaa ctataatccc gacttgttaa gcgcaagtct ttttaatctg gactggaaaa
      601 acaagagtta catttatacc aacagaaccc cttttaaaaa ctcaccaatt tttggcgata
      661 gtggttctgg ttcttatcta tatgataaag aacaacaaaa atgggttttc catggtgtta
      721 ccagtacagt tggttttatc agtagtacca atatagcctg gacaaactac tcgttattta
      781 ataatattct ggtaaacaat ttaaaaaaga atttcacaaa cactatgcag ctggatggta
      841 aaaaacaaga gttatcatcg attataaaag ataaggacct gtctgtctca ggaggagggg
      901 aattaacgct caagcaggat accgatcttg gcattggcgg gcttatattc gataagaacc
      961 agacatataa agtgtacgga aaagataagt cttataaagg tgccgggata gatattgata
     1021 ataataccac cgttgaatgg aatgttaagg gcgttgccgg agataatctg cataaaatag
     1081 gtagtggtac tctggatgta aaaatagcac agggaaataa ccttaaaata ggtaatggga
     1141 ctgtcatcct tagtgctgaa aaagccttca ataaaattta catggccgga ggtaaaggta
     1201 cggtaaaaat aaatgccaaa gacgctttaa gcgaaagcgg taatggcgaa atctatttta
     1261 ccagaaatgg cggaacactg gatctaaacg gctatgacca gtcatttcag aaaatcgcag
     1321 caacagatgc gggaacaacc gtaacgaact caaacgtgaa gcaatcaaca ttatcactta
     1381 ctaatactga tgcatatatg taccatggga atgtatcagg taatataagc ataaatcata
     1441 ttatcaatac tacccagcaa cataacaata atgccaatct gatctttgat ggctcagtcg
     1501 atatcaaaaa cgatatctct gtccggaatg cacagttaac attacaagga catgcgacag
     1561 aacatgccat atttaaagaa ggcaataaca actgtccaat tcctttttta tgtcaaaaag
     1621 actattctgc tgccataaag gaccaggaaa gcactgtaaa taaacgttac aatacggaat
     1681 ataagtccaa caatcagata gcctcttttt cccagcccga ctgggaaagt cgtaaattta
     1741 atttccggaa attaaattta gaaaacgcaa ccctgagtat aggccggggt gctaatgtaa
     1801 aaggacacat agaggctaaa aactctcaaa ttgttctggg aaataaaact gcatacattg
     1861 acatgttctc aggaagaaac attactggcg aaggttttgg attcagacaa cagcttcgct
     1921 ccggggattc agcaggcgaa agtagtttca acggcagtct gagtgctcaa aacagcaaaa
     1981 taactgttgg tgataaatca actgttacta tgactggtgc attatcctta attaatacag
     2041 acctgattat caacaaagga gctactgtta ccgcccaggg aaaaatgtat gtagataaag
     2101 ctattgaact ggccggaacc ctgacattaa caggcacccc tacagaaaat aataaataca
     2161 gcccggcaat ctatatgtca gatggatata atatgacaga agatggtgcc acgttaaagg
     2221 ctcaaaatta tgcctgggtc aatggtaata taaaatcaga caaaaaagca tctattctgt
     2281 ttggtgttga ccagtataaa gaagataacc tggacaaaac cacacacaca ccgctggcta
     2341 caggtttgct gggtggcttt gatacttctt ataccggagg tattgatgct cctgctgcct
     2401 cagccagcat gtataacacc ttatggagag taaacggaca gtcagccctg caatcattaa
     2461 aaacccgcga cagtcttttg ttgtttagta acatagagaa ttcgggtttc catactgtga
     2521 cagtaaacac actggatgcc actaatactg ctgtgattat gcgggctgat ctgagccagt
     2581 ctgtaaatca atcggataaa ctcattgtta aaaatcagtt aaccggaagc aataacagtc
     2641 tgtcggtcga tatacagaaa gtgggaaata ataactcagg attaaacgtt gacctgataa
     2701 cagccccaaa aggaagcaat aaagagatat ttaaagccag tactcaggcc ataggtttca
     2761 gcaacatatc tcctgtgatc agcacgaaag aggatcagga acataccacg tggaccctga
     2821 ccggatataa ggtggctgaa aatacagcat cttccagtgc agcaaaatcg tatatgtccg
     2881 gtaattacaa agccttcctg acagaagtca acaacctgaa taaacgaatg ggggatctgc
     2941 gtgacaccaa tggcgaggtc ggtgcatggg cccgcatcat gagcggagca ggttcagctt
     3001 ctggtggata cagtgacaac tacacccatg tgcagattgg tgtggataaa aaacatgagc
     3061 tggatggact tgaccttttc actggtctga ctatgacgta taccgacagt catgccagca
     3121 gtaatgcatt cagtggcaag acgaagtccg tcggggcagg tctgtatgct tccgctatat
     3181 ttgactctgg tgcctatatc gacctgatta gtaagtatgt tcaccatgat aatgagtact
     3241 cagcgacctt tgctgggctc ggaacaaaag actacagttc tcattccttg tatgtgggtg
     3301 ctgaagcagg ctaccgctat catgtaacag aagactcctg gattgagccg caggcagaac
     3361 tggtttatgg ggccgtatca ggtaaacggt tcgactggca ggatcgcgga atgagcgtga
     3421 ccatgaagga taaggacttt aatccgctga ttgggcgtac cggtgttgat gtgggtaaat
     3481 ccttctccgg taaggactgg aaagtcacag cccgcgccgg ccttggctac cagtttgacc
     3541 tgtttgccaa cggtgaaacc gtactgagtg atgcgtccgg tgagaaacgt atcaaaggtg
     3601 aaaaagacgg tcgtattctc atgaatgttg gtctcaacgc cgaaattcgc gataatcttc
     3661 gctttggtct tgagtttgag aaatcggcat ttggtaaata caacgtggat aacgcgatca
     3721 acgccaactt ccgttactct ttctgataac agcccgggcc gcgtttgcgg cccttcttct
     3781 accggagaga atatgtatta ccctgtgaca gactatatcg ctcttgctct cattattagc
     3841 tttctttttc tgacattatt tatctgcctg ttatgcctta aacatgagcg aataaaaaaa
     3901 gaaactatca ggcaaaaaaa atgcacatat tctggagcat ggctggaatg caactgagtt
     3961 ctcatggttc cgatacggac agtataacga aacgggtatt tacatctcga tcgagaagac
     4021 cattatcatt acaatcaccg tttctggtgg gtgctttaaa aaagaataca gcattgtctc
     4081 ccacatgctg gtcactgaca ccataacaga ggccaccctg tacgaaaacg ggctgtatac
     4141 gcgccacatc agactcagtc gcccagtatc tgacagcaaa taccccttgc cacccggatc
     4201 tcagctcatt aaaaatatga ccttacgact gcgcctgcag gatcagcagg aagaaacatc
     4261 cgtgacatta tttcagggaa aaatgagtac cgacggaaat aactattaca tcatcaaagg
     4321 taaagtaagc tcagtacttc tgttattaaa aatgctgcag attaatcacg cctgatctct
     4381 gtcccggtat tttagcccga ggtatattct gaaatatcat tcatcatgac cggcgtttat
     4441 acaaacgccg gtcattcgct aacagcttca ccgttccttc ccccatccag gttccgcatg
     4501 agcgtgttgt aatgttcata agcgtattat cgtaagcttc cggggaactc atattgacgc
     4561 gtttttcagg cgcgcgtcat cggcggcgca tacgaacgat cgtggtttga taacggttga
     4621 tttattttgt tgcacagggg cgatcgctag gcaggtaccg gaggcgggct ctgaagtact
     4681 gagtagggtg atcgcgtcag gccgcgaagt actgactcgg ggattaatag caggtgatga
     4741 gtaagcgtac agcgagggcc gtattgacgg ggatgtgtta ttcagctggc agtgctatgc
     4801 gccacggaag cagttcgctg acccggttga ccggccagtc tgctatgacg ccaagcacat
     4861 ggcgaaggta gctttctgga tccacgtcat tcagtttgca cgtcccgatc aggctgtaca
     4921 gtagcgctcc ccgctcacca ccatggtcag agccgaagaa caggaagttt ttacgaccca
     4981 gactgaccgc ccgcaaggca ttttcagcga tgttgttgtc gatttccacc cagccatcgt
     5041 tcgcatagta cgtcagtgcc ggccactggt taagtgcgta cgcgaacgcc ttcgccaact
     5101 ctgagtgtcg cgacagggtc ttcatctttt ccgcaaccag ctttccaggg atttcaacag
     5161 cggtttcgtt tttcgctgac accatggagg gagtggttaa aagagcaacg agaaagtggt
     5221 aattataacg ccagccggat atggcgggaa atggtggccc ggggatttac aggcagtgaa
     5281 accatcgtca gggatgcggt tgccaaatgg cgtaaaggct ggaacccacc ggttactact
     5341 gccgtcagac ttccttcagt gtcccgggta agccgctggt tgatgccctg gagaataacc
     5401 agggatgaag aaaattatgc ttcccgattt attagtctga tgtgtgaaaa agaacctgag
     5461 ctgaaaatag cgcagcaact ggcactcgag ttctaccgta ttctgaaaac ccaaaataaa
     5521 tcacagctaa gcagttggtt cactcgagtc cacgaaagcg gttcagcaga atttcggcgc
     5581 gtggcggcgg ggatggaagc tgatgctgca gctatatgtg aggcaatcag tagtcgctgg
     5641 agt
//
LOCUS       DAJVUJ010000213         5596 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.213, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000213 DAJVUJ010000000
VERSION     DAJVUJ010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.213"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(<1..401)
                     /locus_tag="OQQ48_003885"
     CDS             complement(<1..401)
                     /locus_tag="OQQ48_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCS1954467.1"
                     /translation="MGILEQEMKRLAQQAGGSHKTVHDRIKLVQRFCERLVLAQNVQI
                     RRVEQLKARYIEGYVRERLAQGITKRSLQNEMAAVRCILKQAGRDRLAQSERLNNRSL
                     GLSGASRNGTKRAITPEHYRHVLETARAKDPG"
     gene            960..1106
                     /locus_tag="OQQ48_003886"
     CDS             960..1106
                     /locus_tag="OQQ48_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1954468.1"
                     /translation="MYRIKTLASVAMFCDPVFAEVCTGKRIDRPDNRRFFQVVKRWAL
                     STTA"
     gene            complement(1266..1583)
                     /locus_tag="OQQ48_003887"
     CDS             complement(1266..1583)
                     /locus_tag="OQQ48_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016161430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCS1954469.1"
                     /translation="MDRGEIWLVSLDPTTGHEQSGKRPVLIVSKASFNTFTRLPVVVP
                     VTSGGNFAREAGFTVSLDGAGTKTTGVIRCDQPRTIDMGARSGKRLERIPDAVVNEVL
                     ARL"
     gene            complement(1584..1841)
                     /locus_tag="OQQ48_003888"
     CDS             complement(1584..1841)
                     /locus_tag="OQQ48_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000557619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HCS1954470.1"
                     /translation="MYTTRLKKVGGSVMLAVPPAVLKTLGLSTDSEVGMTIDNGCLII
                     EPQKRPRYSLEELLAQCDPHAEISEEGREWIDAPAVGKEIL"
     gene            complement(2190..2396)
                     /locus_tag="OQQ48_003889"
     CDS             complement(2190..2396)
                     /locus_tag="OQQ48_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003842122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS1954471.1"
                     /translation="MMLQDWHPADIIAGLKKRGTSLSALSRQAGLASSTLANALTRRW
                     PKGERLIAEALGIKPEQIWPSRYR"
     gene            complement(2393..2854)
                     /locus_tag="OQQ48_003890"
     CDS             complement(2393..2854)
                     /locus_tag="OQQ48_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1954472.1"
                     /translation="MATIPTQTHPLLAVPFSAATDFTVLADHCENVAETLIESEDPVL
                     RLALCGRLAACLTLLQPTLNDPIPSHLTDSLTVDALPADMPRFVPDAELLCDYSLTLA
                     QLLIGQGMQPKVEQTITGLLCELVWYFAAEMKAPRWLRTADGVKFIDEVAA"
     gene            complement(3225..4829)
                     /locus_tag="OQQ48_003891"
     CDS             complement(3225..4829)
                     /locus_tag="OQQ48_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1954473.1"
                     /translation="MITKELVDESNFISQLVDIGRFDESYQQSVAFLNKLERITIRND
                     NYFIVLANIAGALVDIGQMQNNKNASELGCNLMEDNKEAFISVLGECLYYYNYGNALS
                     NLVSITNPNDHTFKSIEELVSLKNIYWRSFKLSAEEQEEFQAELSVNLANSLRSQFRL
                     SESLRYYDLTNLKGLDIPQAWVNRSMSLMVLNLISSSFSIKLLKEVRAGYIKASVSKN
                     IPPQWESFYLERIAQTNEKIAEYAVDDETDEHDEALTQQEFEVLSPYRQFCLRNHLTL
                     SEHGLYCPCVGSATDDLVIASRGGVTGDFIIPMEMVLNRLKSEFSLARHLYFDYLHPQ
                     NTDYIKYESHFLELYNDEVLGIEIEKIRTAFRLCFGILDKIAVAICELYNLYPTTKKG
                     TPQKNIYFQNFWQLDVDNRRQLFEKVKSPGLLALYSIATDLNKDKGGELAFYKEWRNG
                     LEHKFLVVHKSDKPEDIYESYQLIEDILFIKESDFIHHFEHLLQITRSAIFSFAFMVR
                     QEGMKEKKEGIHYMTRELHAINYSAD"
     gene            complement(4842..5207)
                     /locus_tag="OQQ48_003892"
     CDS             complement(4842..5207)
                     /locus_tag="OQQ48_003892"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1954474.1"
                     /translation="MVSDKRADIIVSLPQIKIPIEIKRDYHRDVWTALNGQLDKLYTK
                     NPDAAGHGIYLVFWFGSARPNSLPHLAKNTSQPENASAMENMLNETVPVDKRDRLSAI
                     VIDVSCEGIPPVEAPKSSV"
     gene            complement(5235..5444)
                     /locus_tag="OQQ48_003893"
     CDS             complement(5235..5444)
                     /locus_tag="OQQ48_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001044500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1954475.1"
                     /translation="MNKTPANIMDLYSLLLDHIRDISNRIANENTDIYKQFWNEDSYG
                     RTLTPKPEESCRNIFLDLLRVRLNP"
BASE COUNT         1447 a         1346 c         1306 g         1497 t
ORIGIN      
        1 cccggatctt ttgcgcgggc ggtttccagc acatggcggt aatgctccgg cgtgatggcc
       61 cgcttcgtac cgttacggga tgcgccagac aggcccagcg agcggttatt cagtcgctca
      121 ctctgcgcca gcctgtcacg cccggcctgt ttcagaatgc agcgcaccgc cgccatctca
      181 ttttgcaggg aacgcttcgt gataccctgc gccagtcgtt cacgaacata tccctcaata
      241 taccgcgcct taagctgctc cacccggcgg atctgcacat tctgtgccag aaccagacgc
      301 tcacaaaacc gctgcaccag cttaatacgg tcgtgaacgg tcttatggct gccgcccgcc
      361 tgttgtgcca atcgcttcat ttcctgctcc agaatcccca tacattcccc ctttcatgat
      421 tgccgcaaac tcatccccaa aaactcaaca ctgatacagc gttccccggc gcacaggccg
      481 atagcccctg agttttgtcg gggctgcgaa cgatacctga gcgccggggc ggcagccgtt
      541 gaggtgtggt ggggcttcgg ttgtgctctc agagcagccg cccgcttgcg cgggtcatcc
      601 cccggatcgt ccagatccgc ctcggtatcg gttcatattc tccttatggg taaatggcct
      661 aaaaaggcgg tgtcagaatc cagccatgag ctggcgcagt tgagttttct ggtgcaggtc
      721 gaaagggctt gcgtgcgtca ctttcccgct ttgcgcggtt aaagtgacgc acggatctgg
      781 tgcagcatct gagatttatc gtctggtgca gttttacggt cacgcggcag caaagcctga
      841 cggcaatgca ggccgcagcc cgtaaggtaa aaggcgctga gggtgtcagc agatgcaggt
      901 tagccgggag aattccggcg cggtgcagat tcagaccagc aggctggcga agtaaaagga
      961 tgtatcgtat taaaactctg gcctccgtcg cgatgttctg cgacccggtg tttgcagagg
     1021 tctgcaccgg taaacggatc gaccgtcctg ataacaggcg cttctttcag gttgtgaagc
     1081 gttgggcgct gtctacgaca gcatagagca agggggagac tacgcctgtg ccgctggcgc
     1141 ggaaaggaga aactgttttt acctgcaaaa ggatttttta atcacttgtt gttataaaaa
     1201 atgagggcgc tgtatgtgac agcgccctcg ttcaggttac ataaaaaata tcagctcaga
     1261 atagcttaca gtcgagccag cacctcattc acgacagcat cgggtatacg ttccagccgc
     1321 ttaccgctcc gagctcccat atcaatggtt ctgggctggt cgcagcgaat aacgcccgtt
     1381 gtttttgttc ccgccccgtc cagtgaaacg gtaaaaccgg cttcgcgggc aaagtttccg
     1441 ccgctggtga cagggacaac aacaggcaac cgggtgaacg tgttaaacga tgccttcgat
     1501 acgatgagta ccggacgttt tccactttgt tcatggccag tggtgggatc cagtgaaacg
     1561 agccagattt ctcctctgtc catctacagg atctccttgc ccaccgcagg cgcatcaatc
     1621 cattcccgac cctcttcgct tatttcagcg tgcggatcgc actgcgccaa cagttcttcg
     1681 agcgaatagc ggggacgttt ttgaggttca ataatcaggc agccattatc aatggtcatg
     1741 cccacttcgc tgtccgtcga cagccctagc gttttcagca ctgcgggggg aaccgccagc
     1801 atgacggatc cgccaacctt tttcaggcga gtggtataca tagagcacct ccggatatta
     1861 tattttaata taacatccat gatagacgat gcacaactat tcatcagatt cattgtggtt
     1921 tccttagtgt aataaagcgt gaagaaagcc ttaatccatc ggaagacaga tccggcagtt
     1981 ttcaaaggcg acattgccgg taaaagggca accgtcctga tacatcaggc gcttctttca
     2041 ggtcatgaag catttcgtac aggcgggagc ttagtgcgta acaaagaagg ccgataatag
     2101 gaaactgctt ttgcagttca aaggattatg acggttcgga taaacaaaat gcggcgcagc
     2161 ttccgtatcc gggctgcgcc gcaggaacat cagcggtagc gcgaaggcca gatttgctca
     2221 ggcttgatgc ctaacgcttc ggcaattaac ctttcgcctt tgggccagcg gcgggtaagc
     2281 gcattagcca gcgtggagga cgccagacct gcctgacgcg aaagcgctga cagcgaagtg
     2341 cctcgttttt tcagcccggc gataatgtcg gcaggatgcc agtcctgcag catcatgcag
     2401 ccacctcgtc gataaacttc acgccatcgg cggtgcggag ccagcgcggg gctttcattt
     2461 cggcggcaaa ataccagacc agttcacata ataagcctgt tatcgtctgc tccacttttg
     2521 gctgcatccc ctgcccgata agaagttgcg ccagcgtcag tgagtaatca caaagtaatt
     2581 ctgcgtcggg gacaaatcgg ggcatatcgg caggtagagc atcaacggtc aggctgtcag
     2641 tcagatgaga cgggatgggg tcgttcagcg tgggttgcag caaagtgaga caggcggcaa
     2701 gtcgcccgca aagcgccagc cgcagcaccg gatcttcgct ttcgatcagg gtttcggcga
     2761 cgttttcgca gtggtctgcc agtacggtaa aatctgtggc agcactgaag ggaacagcaa
     2821 gtaacgggtg tgtctgagtg gggatcgtag ccataaaggc agcctccagt atgtggtttg
     2881 aaaaccacca ccagaggttc caatctcatg ggtggtggac tgaacgaggt tggaactacc
     2941 ggccatactg gaaccggcgc accttacggt gcccccgccc agcccaccat aattttggtg
     3001 tagctaagcg cgcgacataa aaaaagacgc agacgcgtca cgtgtcgcca gtatggttat
     3061 atttcggggt tccaatcccg gcacctgatt ttgcaggtgc gggtaaaaga taccgctttc
     3121 ggttgaaaac ggcaagggga agtcgtggaa tagagatcgc agattgttat aaatatacac
     3181 aatagttaat gttaacaaag acggcacata tattaacttt actctcaatc tgctgaatag
     3241 tttatcgcat gtaattctcg ggtcatatag tggataccct ccttcttttc tttcatcccc
     3301 tcttgcctca ccataaaagc aaacgaaaaa attgctgaac gagttatctg aagtaaatgc
     3361 tcaaaatggt gaataaaatc actttctttg ataaaaagaa tatcttctat tagttgataa
     3421 gattcgtaaa tgtcctcagg tttatcactt ttatgtacaa ctaaaaactt atgctctaaa
     3481 ccattgcgcc attctttata gaaagccaac tccccaccct tatctttatt caggtcggta
     3541 gcaatactgt acagcgccag aagccctggt gattttacct tttcaaataa ttgtcttctg
     3601 ttatcaacat ctaactgcca gaaattctga aaatagatat tcttctgagg tgtacctttt
     3661 tttgttgttg ggtaaagatt ataaagttca catatggcta ccgcaatttt atcaagaata
     3721 ccaaagcaaa gacgaaatgc cgtcctgatt ttttcaattt ctattcctaa aacttcgtca
     3781 ttatacaatt ccagaaaatg gctttcgtac ttaatgtaat ccgtattttg tggatgaaga
     3841 taatcaaaat aaagatggcg tgcgagtgaa aactccgatt taaggcgatt taataccatc
     3901 tccatgggaa tgataaaatc acctgttaca ccgcctcgcg aagcaatcac aagatcatct
     3961 gtagcactcc ctacacatgg gcaatacagg ccatgttctg ataaagtcag atgatttcta
     4021 agacaaaact gtcgatatgg gcttaacact tcgaactctt gttgtgtaag tgcttcatcg
     4081 tgttcgtcag tttcatcatc aacagcatat tctgcgattt tctcgttagt ctgcgctatg
     4141 cgttctaaat agaacgattc ccattgtggt gggatatttt ttgatacaga cgctttaata
     4201 tatcctgctc tcacttcttt tagtaacttg attgaaaatg aagatgaaat aagattgagg
     4261 accataagtg acatcgaacg gttgacccaa gcctgtggta tatctaatcc cttaagattg
     4321 gtaagatcat aatatctcaa tgactcactg agccgaaatt gacttcttaa tgaattagcc
     4381 aaattaacag atagttcagc ctgaaattcc tcctgctcct cagcagatag cttaaaagaa
     4441 cgccagtaga tattttttaa ggaaaccaat tcttctatcg atttgaatgt atggtcattg
     4501 gggttagtaa tgctcaccag attagatagc gcattaccat agttataata gtaaaggcac
     4561 tctcctaaca ctgatataaa tgcttctttg ttatcttcca tcaggttgca accaagctct
     4621 gacgcatttt tattattttg catctgcccg atgtcaacaa gagcacccgc aatgttcgcc
     4681 aatactataa agtagttatc gttcctgatt gttattcttt ccagtttgtt caggaacgca
     4741 actgactgtt gataagattc gtcaaatctt ccaatatcaa ccagttggct aatgaaatta
     4801 ctttcatcga caagttcttt tgttatcaaa ataaattttc cttatacaga ggatttcggt
     4861 gcttcaacag gaggtatgcc ctcacaggag acatctataa ctatcgctga taagcggtct
     4921 cttttatcaa ccggcacagt ttcattaagc atattttcca ttgcactggc gttttcaggt
     4981 tgagatgtat ttttcgccaa atgaggcagg gaattcggtc gagcagaacc aaaccaaaaa
     5041 accagataga taccgtgacc agcagcatcg gggtttttag tataaagctt gtccaattgc
     5101 ccattaagtg cagtccagac atcacgatga taatcacgct ttatctcgat aggtattttt
     5161 atctgcggta gtgaaacaat gatatctgcc cttttatcag aaaccatgtg tccttctggt
     5221 tcacagctta ttttttatgg attaagccgg acacggagta aatccagaaa aatattacgg
     5281 caactctctt caggtttagg cgttagggta cggccatagc tgtcttcatt ccagaattgc
     5341 ttataaatat cggtattttc attggcaatt cggttagaaa tatcccgtat atgatcaagc
     5401 aataatgagt aaagatccat tatgttggca ggcgttttat tcatcaatgt acttacagtc
     5461 tgcttccagt cggcatgatg gaactgtgat tctctataac gaattttctg attgctttgg
     5521 gcatgtgaga gatgatcccg ataactttca cattcaggca gcagtaataa gcgctcaagt
     5581 acttcactag catcgt
//
LOCUS       DAJVUJ010000214         5591 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.214, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000214 DAJVUJ010000000
VERSION     DAJVUJ010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5591
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.214"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(35..772)
                     /gene="bamD"
                     /locus_tag="OQQ48_003894"
     CDS             complement(35..772)
                     /gene="bamD"
                     /locus_tag="OQQ48_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HCS1954476.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENSYRQMQMNAQAEKVAKIIAANSSNT"
     gene            907..1887
                     /gene="rluD"
                     /locus_tag="OQQ48_003895"
     CDS             907..1887
                     /gene="rluD"
                     /locus_tag="OQQ48_003895"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HCS1954477.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNLDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPEDMVELIEVMRADFEEHKDEVDWL"
     gene            1884..2615
                     /gene="pgeF"
                     /locus_tag="OQQ48_003896"
     CDS             1884..2615
                     /gene="pgeF"
                     /locus_tag="OQQ48_003896"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HCS1954478.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     EHVEENRKQLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2745..5318
                     /gene="clpB"
                     /locus_tag="OQQ48_003897"
     CDS             2745..5318
                     /gene="clpB"
                     /locus_tag="OQQ48_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCS1954479.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1391 a         1335 c         1516 g         1349 t
ORIGIN      
        1 ctcaagggct gccgttttgt gtttcaggtt tctgttatgt attgctgctg tttgcggcga
       61 tgattttcgc tactttttca gcttgcgcat tcatctgcat ctgacggtat gaattttcca
      121 tcagtggcag cgcatcacgc gtagcctggg tatccgggta gtcgcgcaac atgccttcta
      181 cgcggttaac gacggcaacc catgcaccac gttctgtata gtactcggca acggagtatt
      241 catatttcgc cagacgatct ttcaggaata ccagacgttt ggttgcatcg gtagtgtact
      301 gactgttcgg atagccgcgc accagtttgg aaaagtcact aaacgcagct cgtgcatgtt
      361 gaggatcgcg atcgctacgg tcaacgccaa agaacccttg cagcgcactg tcatccagcg
      421 ccatattggt caggccacgc atgtacatga cataatcgat attcggatgg gtcgggttaa
      481 ggcgaataaa acgatcgatg gcagcctgtg ctaacggcaa atcggcgttt ttatagtagg
      541 cgtagatgag atccagctgc acctgctgcg aatacggacc aaacggatag cgattatcta
      601 atgcttccag ttgcgttatt gcctgtctcc agttaccgtc ctgcagcttt tgttgtgcag
      661 tcgcgtaaat ttcatttggt ggattatcag gtacttcttc ctttgacccc gagcaacccg
      721 ccaaaaacag gcttagtgtg gcggctgcca ccagatattt catgcgcgtc atgacgtttt
      781 gactttcctc aaaatgtaat acgggagatt ctctgttcct gctcccggtt aagaccagct
      841 acaatagcac actatattaa acggcaaagc cgtaaaaccc caacgataaa cgaagaagca
      901 gtatatatgg cacaacgagt acagctcact gcaacggtgt ccgaaaacca actcggtcaa
      961 cgcttagatc aggctttggc cgaaatgttc ccggattatt cacgttcgcg aataaaagaa
     1021 tggatcctcg accagcgcgt gctggtaaac ggcaaagttt gtgataagcc gaaagaaaaa
     1081 gtattgggtg gcgagcaggt tgccatcaac gctgagattg aagaagaagc gcgttttgaa
     1141 ccgcaggata tcccgctgga tatcgtctat gaagatgaag atatcatcgt cattaataaa
     1201 ccgcgcgacc tggtggtaca tcctggcgcg ggtaacctgg atggcacggt actgaatgcg
     1261 ttgcttcatt attatccgcc cattgccgat gtaccgcgtg cgggcatcgt ccatcgtctg
     1321 gataaagaca ccactggcct gatggttgtg gcaaaaaccg ttccggctca gacgcgttta
     1381 gtcgaatctt tgcaaaggcg tgaaattact cgtgagtatg aagcggtggc aattggtcat
     1441 atgaccgctg gcggtacagt agatgaacca atcagccgcc acccgaccaa acgtactcac
     1501 atggcggtgc atcccatggg caaaccggcg gtcactcact atcgcatcat ggaacacttc
     1561 cgtgtgcaca cgcgtctgcg gttgcgtctg gaaaccggac gtacgcacca gatccgcgtg
     1621 catatggcgc atattaccca tccgctggtg ggcgatccgg tttatggtgg ccgtccgcgt
     1681 ccgccaaaag gtgcttcgga agcgtttatc tccacgctgc gtaagtttga ccgccaggcg
     1741 cttcatgcaa ctatgctgcg tctttatcac ccgatctccg gcatcgaaat ggaatggcat
     1801 gcgcctattc cagaagatat ggtggagttg attgaggtta tgcgcgccga tttcgaagag
     1861 cataaggatg aagtggactg gttatgagta agctgattgt cccgcagtgg ccgctgccaa
     1921 aaggtgttgc ggcctgtagc tctacccgta tcggcggcgt gagcttgcca ccttacgact
     1981 ctctcaacct tggcgctcat tgtggtgata acccggagca cgtagaggag aatcgcaagc
     2041 aactttttgc tgcgggaaat ttgccttcaa aaccggtctg gcttgagcag gtacacggca
     2101 aagatgtgct taagctcact ggcgaacctt atgcctcaaa acgggcggat gcctcttata
     2161 gcaatacgcc cggcacggtt tgcgcagtga tgactgccga ctgcctccct gtgctgtttt
     2221 gcaatcgagc gggaacggaa gtcgccgccg ttcatgctgg ctggcgtgga ctgtgcgcag
     2281 gcgtgctgga agagacggtt tcctgttttg ctgataaacc tgaaaacatt ctcgcctggt
     2341 tagggccggc aattggtcca cgcgcgttcg aagtgggggc ggaggttcgc gaggcgttta
     2401 tggcagtaga cgctaaagca agtgcagctt tcattcagca tggtgataag tatctggcgg
     2461 atatttatca gcttgcccgg cagcgtctgg cgaacgtggg tgttgagcaa attttcggcg
     2521 gcgaccgttg tacatatacg gaaaatgaga ctttcttctc ttatcgtcgc gacaagacca
     2581 ccggtcgtat ggcaagtttc atttggctga tataacctaa agaatcaaga cgattcggta
     2641 cgcgtgattt tcttttcaca ttaatctggt caataacctt gaataattga gggatgacct
     2701 catttaatct ccagtagcaa ctttgatccg ttatgggagg agttatgcgt ctggatcgtc
     2761 ttactaataa attccagctt gctcttgccg atgcccaatc acttgcactc gggcacgaca
     2821 accaattcat cgaaccactt catttaatga gcgccctgct gaatcaggaa gggggttcgg
     2881 ttagtccttt attaacatcc gctggcataa atgctggcca gttgcgcaca gatatcaatc
     2941 aggcattaaa tcgtttaccg caggttgaag gtactggtgg tgatgtccag ccatcacagg
     3001 atctggtgcg cgttcttaat ctttgcgaca agctggcgca aaaacgaggt gataacttta
     3061 tctcgtcaga actgttcgtt ctggcggcac ttgagtctcg cggcacgctg gccgacatcc
     3121 tgaaagcagc aggggcgacc accgccaaca ttactcaagc gattgaacaa atgcgtggag
     3181 gtgaaagcgt gaacgatcaa ggtgctgaag accaacgtca ggctttgaaa aaatatacca
     3241 tcgaccttac cgaacgagcc gaacagggca aactcgatcc ggtgattggt cgtgatgaag
     3301 aaattcgccg taccattcag gtgctgcaac gtcgtactaa aaataacccg gtactgattg
     3361 gtgaacccgg cgtcggtaaa actgccatcg ttgaaggtct ggcgcagcgt attatcaacg
     3421 gcgaagtgcc ggaaggattg aaaggccgcc gcgtactggc gctggatatg ggcgcgctgg
     3481 tggctggggc gaaatatcgc ggtgagtttg aagaacgttt aaaaggcgtg cttaacgatc
     3541 ttgccaaaca ggaaggcaac gtcatcctgt ttatcgacga attacatacc atggtcggcg
     3601 cgggtaaagc cgatggcgca atggacgccg gaaacatgct gaaaccggcg ctggcgcgtg
     3661 gtgaattgca ctgcgtaggt gccacgacgc ttgacgaata tcgccagtac atcgaaaaag
     3721 atgctgcgct ggaacgtcgt ttccagaaag tgtttgttgc cgagccttct gttgaagata
     3781 ccattgcgat tctgcgtggc ctgaaagaac gttacgaatt gcaccaccat gtgcaaatta
     3841 ctgacccggc aattgttgca gcagcgacgt tgtctcatcg ctacattgct gaccgtcagc
     3901 tgccggataa agccatcgac ctgatcgatg aagcagcatc cagcattcgt atgcagattg
     3961 actcaaaacc agaagaactc gaccgactcg atcgtcgtat catccagctc aaactggaac
     4021 aacaggcgtt aatgaaagag tctgatgaag ccagtaaaaa acgcctggat atgctcaacg
     4081 aagaactgag cgacaaagaa cgtcagtact ccgagttaga agaagagtgg aaagcagaaa
     4141 aggcatcgct ttctggtacg cagaccatta aagcggaact ggaacaggcg aaaatcgcta
     4201 ttgaacaggc tcgccgtgtg ggggacctgg cgcggatgtc tgaactgcaa tacggcaaaa
     4261 tcccggaact ggaaaagcaa ctggaagccg caacgcagct cgaaggcaaa actatgcgtc
     4321 tgttgcgtaa taaagtgacc gacgccgaaa ttgctgaagt gctggcgcgt tggacgggga
     4381 ttccggtttc tcgcatgatg gaaagcgagc gcgaaaaact gctgcgtatg gagcaagaac
     4441 tgcaccatcg cgtcattggt cagaacgaag cggttgatgc ggtatctaac gctattcgcc
     4501 gtagccgtgc ggggttggcg gatccaaatc gcccgattgg ttcattcctg ttcctcggcc
     4561 caacgggtgt ggggaaaact gagctttgta aggcgctggc gaactttatg tttgatagcg
     4621 acgaggcgat ggtccgtatc gatatgtccg agtttatgga gaaacactcg gtgtctcgtc
     4681 tggttggtgc gcctccggga tatgtcggtt atgaagaagg tggctacctg accgaagctg
     4741 tgcgtcgtcg tccgtattcc gtcatcctgc tggatgaagt ggaaaaagcg catccggatg
     4801 tcttcaacat tctgttgcag gtactggatg atggtcgtct gactgacggg caggggagaa
     4861 cggtcgactt ccgtaatacg gttgtcatta tgacctctaa cctcggttcc gatttgattc
     4921 aggaacgctt cggtgaactg gattatgcgc acatgaaaga gctggtgctc ggtgtggtaa
     4981 gccataactt ccgtccggaa ttcattaacc gtattgatga agtggtggtc ttccatccgc
     5041 tgggtgaaca gcacattgcc tcgattgcgc agattcagtt gaaacgtctg tacaaacgtc
     5101 tggaagaacg tggttatgaa atccacattt ctgacgaggc gctgaaactg ctgagcgaga
     5161 acggttacga tccagtctat ggtgcacgtc ctttgaaacg tgctatccag cagcagatcg
     5221 aaaacccgct ggcacagcaa atactgtctg gtgaattggt tccgggtaaa gtgattcgcc
     5281 tggaagttaa tgaagaccgg attgtcgctg tccagtaatg ataaaacgag cccttcgggg
     5341 ctcgtttttg tctataagtt agacggaaaa gactatattt aagatgtttt gcctgaaaaa
     5401 tgagcgaacg ataaagtttt tatatttttc gcttgtcagg ccggaataac tccctataat
     5461 gcgccaccac tgacacggaa caacggcaaa cacgccgccg ggtcagcggg gttctcctga
     5521 gaatctcaac agagaaaagc aaaaaaatgc ttgactctgt agcggggaag cgtattatgt
     5581 acaccccgcg c
//
LOCUS       DAJVUJ010000215         5519 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.215, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000215 DAJVUJ010000000
VERSION     DAJVUJ010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5519
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.215"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(336..599)
                     /locus_tag="OQQ48_003898"
     CDS             complement(336..599)
                     /locus_tag="OQQ48_003898"
                     /inference="COORDINATES: protein motif:HMM:NF033564.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HCS1954480.1"
                     /translation="MDTHYYVSSLEIAPEQAAKAVRQHWHIENQQHWVLDVAYREDAS
                     RLGDREAAGFFALFRRILRPSNRMSHLGKLWFRTHYSPNKKRL"
     gene            865..1158
                     /locus_tag="OQQ48_003899"
     CDS             865..1158
                     /locus_tag="OQQ48_003899"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1954481.1"
                     /translation="MIGILKESPKDPDSLKVISITTPARGFSKYGFGSTEHGWFTTIT
                     YNAKNSYGGYVGAKSITFVYLNGDYKVAVPMSNGERVMLDKVSFSCKDNCQTK"
     gene            complement(1186..1341)
                     /locus_tag="OQQ48_003900"
     CDS             complement(1186..1341)
                     /locus_tag="OQQ48_003900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1954482.1"
                     /translation="MNSKQKCSHSNLEKEYYLGSATGDYKCLDCGEVVFGKDWPEKER
                     SKNDNEE"
     gene            2304..2453
                     /locus_tag="OQQ48_003901"
     CDS             2304..2453
                     /locus_tag="OQQ48_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1954483.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGPPV
                     GREAL"
     gene            3523..3747
                     /locus_tag="OQQ48_003902"
     CDS             3523..3747
                     /locus_tag="OQQ48_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraY domain-containing protein"
                     /protein_id="HCS1954484.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYY
                     LSAETAARVRRGDEAVHSSEDVRKSLGLDD"
     gene            3731..3997
                     /locus_tag="OQQ48_003903"
     CDS             3731..3997
                     /locus_tag="OQQ48_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCS1954485.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMDLRIAVAADPRQSG
                     KPLKGELGEFWRYRVGDYRILCEIRDDELVILAATIGHRREVYD"
     gene            complement(4044..4313)
                     /locus_tag="OQQ48_003904"
     CDS             complement(4044..4313)
                     /locus_tag="OQQ48_003904"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1954486.1"
                     /translation="METITHPTLVQLVAAGAVRVVIAVGQPGGWTLLVRYGLAERALA
                     AQRSKQLRVFRKLDTLVLYLQQIGVCRFEVDATGFMGAPRQIRSS"
     gene            complement(4501..4599)
                     /locus_tag="OQQ48_003905"
     CDS             complement(4501..4599)
                     /locus_tag="OQQ48_003905"
                     /inference="COORDINATES: protein motif:HMM:NF015750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1954487.1"
                     /translation="MATIIVHCPRCQSDCVCRHGKGAVSVPCWRVF"
     gene            complement(4641..>5519)
                     /locus_tag="OQQ48_003906"
     CDS             complement(4641..>5519)
                     /locus_tag="OQQ48_003906"
                     /inference="COORDINATES: protein motif:HMM:NF013884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCS1954488.1"
                     /translation="IISGFIMCYTTHNKNLTIISFLTLRVQRIIPLYWVFTTLALTVF
                     VISPSLVNSSGGETSIIDSYFLIPSSAKYLVNNGWTLSYEFYYYLIFSLFLVAFSSTK
                     LRYACIIATLLILSSFGLIFQPEDIVVDFLLSSWLYEFAIGVILFVAYDRGLVKANFG
                     YISVTLALAFLLYKNFNMEYLSGAPRVISIGVPMGLLFYGFLSFEGYFKRKKHFISNL
                     FERIGDSSYSLYLSHPFVLSPMAIIASKLGVTNSFLFGVILIGSSVFIGYATYVIIEK
                     RIIYFTKIKLVMPSVG"
BASE COUNT         1541 a         1257 c         1216 g         1505 t
ORIGIN      
        1 aaaaaaaaga tcgacccccg acccgcctat atgaaaccaa cttaaagttt ctgtattgta
       61 aatatttcct ttatgagcag tatgagccat aacgacaaga agagcagcaa taccccgcaa
      121 aaactgtata gaatataaca tcgaccacct aattccttac attatagttc atataaccta
      181 accatcttaa gcaagtgtta gaaatgggta cttaatggac accaaaaaaa tttaagcttt
      241 ccaataagtt agctagctaa aggccatgat tggcccaata atagtattct accaataaac
      301 caccagttaa tacaccgcca acggcacccg ctgttttata aacgcttctt gtttggcgag
      361 taatgagttc tgaaccagag cttacccaga tgcgacatcc tatttgaggg gcgcaggata
      421 cggcgaaaca gggcaaaaaa ccctgcagct tctctatctc cgagccgact ggcatcttcc
      481 cgatacgcca catccagcac ccaatgttgc tgattttcaa tgtgccaatg ctggcgaacc
      541 gctttggctg cctgttccgg cgctatctcc agcgagctga cgtaataatg ggtatccaag
      601 cttagagcct atcccaatag gctcttaaag tcggcaacgt ctatttattt tcacagcgca
      661 taacggatgt tatggtaaat ctatctataa atatctattg tagtggattt cctatagttg
      721 ttttggaaaa tgacaaggat atatatgaaa ataattaagt gggcttcact gtctggtgtt
      781 ctgttaatat ctgcttgtgg tgtttcttag aacgaagtaa atagtgctaa ttatggttta
      841 aaaccaatag ataatgatgc gaaagtgatt ggtatactta aagagagtcc aaaagatcct
      901 gattctctaa aagttatatc aataaccacc cccgccagag gtttttctaa gtatggcttt
      961 ggctctacag agcatggctg gttcactact atcacatata atgcaaaaaa ctcttatggc
     1021 ggttatgttg gtgctaagtc cattactttc gtttacttaa acggagatta taaagtggca
     1081 gtgccaatgt ctaatggtga gagggttatg ttagataaag tgtcattttc atgtaaagac
     1141 aattgccaaa ctaaatgata aaaaaggtcg caatagcggc tttttttatt cttcgttatc
     1201 atttttactt ctttcttttt ctggccagtc cttcccaaaa acaacctccc cgcaatccaa
     1261 acatttataa tctcctgtgg cgctacccaa gtagtattct ttttctagat ttgaatgaga
     1321 acatttttgt ttgctgttca ctacagtcac ctttggatat tcatttattc gtgaatgatt
     1381 tttatctata ccgacattgt taaatgatgg aaagatcttc ttgagatcct ttttttctac
     1441 gcgtaatctt catgactgta aacgaaaaaa ccaccctggc aggtggtttt tcgaaggtta
     1501 gttaatcctg gcagattctc taaccgtggt aacagtcttg tgcgagacat gtcaccaaat
     1561 ctgtccttcc agtgtagcct cagtcaggcc gccacttcaa gaactctgaa cacatctctc
     1621 gcacaaacct gttaccagtg gctgctgcca gtggcgtttt gtcgtgtctt tccgggttgg
     1681 actcaagacg atagttaccg gataaggcgc agcagtcgga ctgaacgggg ggttcgtgca
     1741 tacagtccag cttggagcga actgcctacc cggaactgag tgtcaggcgt ggaatgagac
     1801 aaacgcggcc ataacagcgg aatgacaccg gtaaaccgaa aggcaggaac aggagagcgc
     1861 acgagggagc taccaggggg aaacgcctgg gatctttata gtcctgtcgg gtttcgccgc
     1921 cactgatttg agcgtcagat tttgtgatgc ttgtcagggg ggcggagcct atggaaaaac
     1981 ggcagcgccg catccttttt ccggccttcc ctgacttgag tgccatctct ttatcttgtc
     2041 attcgtgagc aggtaacgcc cgccgcagcc tcacggcagg agcgtagcga ccgagtgagc
     2101 gaggaggcgt catatcaccg taactcccat tttctccctt acgccctgta gctgcatttt
     2161 cccctgtcac atgaagcact ttcattaggt gctactccgc cgtaacatat tcagccactg
     2221 tacactccgc tagcgctacg tgactggttc agtgctgcgc tccgaaaccc gctaacaacc
     2281 tctccgctac tagatatcac cccatgccat ctgaatatga tgaacaactg gattcgactg
     2341 atacgcagct tcccggcgaa tatgaccttt ttgatttaat ggcgcaggtt actgaagaga
     2401 acctgcacgc cccggtggat tttgggccgc ctgtcggccg ggaagcgtta tagctttttc
     2461 gcttacccgc gtacgtgtaa tacgccggta ttacatctta tttctttatt ttcatgtgat
     2521 tacaggtggt tgtaatacgt tgcgtattac ataaataaac gggaacttca gtttttacct
     2581 tttaattcat gcaattgcga tgaaaatcgc ggttgcgtat ggtgtatggc catttaaggg
     2641 ataaagaatc atctccttac cgggctgagc tttgcctatt ctgaggagat ctgtgaaaaa
     2701 aagcgtccat aactagctcg ttcatacttg tatgaacagc ttattcctcc tgactactaa
     2761 taaactacag cgtcactact gatttactac aaatcggagg aggatgacta cttatctact
     2821 actgatttac tactgaatta ctactgaatt acgtcataac agttctctga accattcgta
     2881 catccgaccc ccttctcttc atgcttgatg ctcttgccaa cgcttcacgg tcacgcaata
     2941 aattgcgtcc ccgttcaccg ttttcggatg agaagaacgt cgaaaacagg acttccactg
     3001 gctgcacggc gtgaaattct gcgctatgca cttgcgcgca tactcatgca tgccgtaaaa
     3061 acagagcctg cgtgtttctg gcgggttttc gggtggtttg ttgccttgtt ttaccggttt
     3121 cccgtcagaa acgctctcag agcctctcag gcggtgcacg caacagatgt tatgttcaat
     3181 cagactgatc tgcgggtaca aaaaaagccc cgggggcttt taactactga cttatttttt
     3241 tgaggatttg ctgggtttca atttgtcagt attccccggc gtttttttat tgtcacgctg
     3301 gcgtttatca ggttcccctg ttgaatcggc aattcgtttc ggacccggct taagacctat
     3361 aatcgttatc ctttcaatag ttaagaggaa agccctcaag ctggatatat tgacagcgtt
     3421 acggattatc aagaggggcc agcaagatta ttttttgttc ttttaggttg attgcgatac
     3481 tattgctata catgtatatc aaatggtgtt caggaggttt atatgcttgc tatccggtta
     3541 tctgatgaga ttgagtcccg tctggactcg ctggcgaagc aaaccggcag aacaaagacg
     3601 ttctatgcgc gggaagcaat actggcgcat ctggaagacc tggaagatta ttatctttca
     3661 gcagaaactg ccgcacgcgt tcgccgtggt gatgaagccg tgcattcgtc tgaagacgtg
     3721 aggaagtcac ttggtctgga cgattaacta ttccgaccgg gcgctcaaat cgttacgcaa
     3781 gatggacaaa cagaacgcac gacggattgt ggattttatg gatttacgca ttgcagttgc
     3841 tgccgatcct cgccagtcag ggaagccgct caaaggtgag ctgggcgagt tctggcgcta
     3901 tcgggtggga gattatcgca ttctgtgtga gatccgggat gacgagctgg ttatccttgc
     3961 cgccaccatt ggccatcgcc gcgaagttta cgactgaagc ccgctatgcg ggcttttttt
     4021 gatgctgata acgcctttta tgttcaagag ctcctgatct gtctcggggc acccatgaag
     4081 cccgtggcat ccacctcgaa ccggcaaacc ccgatctgct gcaggtagag aaccagggta
     4141 tcgagcttgc gaaaaactct gagctgcttg ctgcgctggg cggccagggc acgttcggct
     4201 agaccgtagc gcaccagcag cgtccagccg ccaggttggc caactgcgat cacgacacga
     4261 acagcaccgg ccgccaccag ttgtaccagg gtgggatggg taatggtttc catgggggag
     4321 attccgagac aagggatagt gcggcgcagg atgggagata acgcctagca aaacaagtca
     4381 tcttgcagga tgaccagcag caaaaagggc gtccgattta acgtaacgcc cccttatgcg
     4441 cagtcatcca ggcgggttat gactggggtg ggtactgagc aacgtagctg gttgtcagct
     4501 tcaaaagact cgccaacacg gcacggaaac agcacccttt ccgtggcggc agacgcagtc
     4561 tgattggcaa cgtgggcaat gaacgatgat agtggccata aggaatactg agtcttaaaa
     4621 gccaccatgg ctcacgttca tcaacctact gaaggcatca ccaatttaat tttggtgaaa
     4681 taaattattc ttttctcgat aattacgtaa gttgcataac caataaatac ggacgaccca
     4741 attaatataa ccccaaaaag aaatgaattt gtcaccccta acttactagc aataattgcc
     4801 atcggactta aaacgaaagg atgggaaagg tataaggagt aagagctatc tccaatccgt
     4861 tcaaataaat ttgagataaa atgttttttt cgtttaaaat acccctcaaa tgataagaaa
     4921 ccataaaaca gcaaccccat tggaacacca atagatatca ctcttggagc acctgataaa
     4981 tactccatgt taaagttttt atatagcaaa aaagccaatg ccaatgtaac tgagatataa
     5041 ccaaaattag ctttcaccaa ccccctgtca taagcaacaa ataatataac accaatcgca
     5101 aactcataca gccaactgct taacaaaaaa tccaccacta tatcttctgg ctgaaaaatc
     5161 aatccaaaag aggataaaat taagagagta gcaatgatac atgcatatct tagtttggtt
     5221 gacgagaaag caaccaaaaa taacgaaaag attagatagt aataaaattc atagcttaat
     5281 gtccatccgt tatttaccaa gtatttggca ctagatggta ttaaaaaata cgaatctatg
     5341 atgctagttt ctccaccaga tgagttaaca aggctgggag atataacgaa cacagtcaga
     5401 gctagagtcg taaataccca gtaaagagga atgattctct gaacacgcaa cgtcaaaaaa
     5461 cttatgattg ttaaattttt attatgtgtt gtgtagcaca ttataaaacc tgaaataat
//
LOCUS       DAJVUJ010000216         5439 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.216, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000216 DAJVUJ010000000
VERSION     DAJVUJ010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5439
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.216"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..915
                     /gene="adeQ"
                     /locus_tag="OQQ48_003907"
                     /gene_synonym="yicO"
     CDS             <1..915
                     /gene="adeQ"
                     /locus_tag="OQQ48_003907"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HCS1954489.1"
                     /translation="IPLSLRIGITSGIGLFIALMGLKNTGVIVANKDTLVMIGDLSSH
                     GVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCCGLFFGDVHFSGVYSIPPDISGVIG
                     EVDLSGALTLELAGIIFSFMLINLFDSSGTLIGVTDKAGLIDGNGKFPNMNKALYVDS
                     VSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTAVVVGVMFLLVMFFSPLVAMVPPYA
                     TAGALIFVGVLMTSSLARVNWDDFTESVPAFITTVMMPFTFSITEGIALGFMSYCIMK
                     VCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            968..1420
                     /locus_tag="OQQ48_003908"
     CDS             968..1420
                     /locus_tag="OQQ48_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HCS1954490.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            1631..2821
                     /gene="nepI"
                     /locus_tag="OQQ48_003909"
     CDS             1631..2821
                     /gene="nepI"
                     /locus_tag="OQQ48_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HCS1954491.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRCYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(2862..3155)
                     /locus_tag="OQQ48_003910"
     CDS             complement(2862..3155)
                     /locus_tag="OQQ48_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1954492.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            3377..4195
                     /gene="nlpA"
                     /locus_tag="OQQ48_003911"
     CDS             3377..4195
                     /gene="nlpA"
                     /locus_tag="OQQ48_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HCS1954493.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKITTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLHTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            complement(4222..5121)
                     /locus_tag="OQQ48_003912"
     CDS             complement(4222..5121)
                     /locus_tag="OQQ48_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005030206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCS1954494.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLVGTFLLVTHGNPTSLSLSPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRPEK"
     gene            complement(5232..>5439)
                     /locus_tag="OQQ48_003913"
     CDS             complement(5232..>5439)
                     /locus_tag="OQQ48_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1954495.1"
                     /translation="GMLYFQDLMPEKIGSATTLYANTSRVGWIIAGSVDGIMVEIWSY
                     HALFWLAIGMLGIAMICLLFIKDI"
BASE COUNT         1298 a         1238 c         1394 g         1509 t
ORIGIN      
        1 attcccttaa gtttacgtat tggtatcacc agcggaattg gattatttat cgccttaatg
       61 ggattaaaaa atactggcgt tattgtcgcc aataaagaca cgctggtgat gattggcgat
      121 ttaagttctc acggcgtgtt gttaggtatt ttagggtttt ttattataac cgtgttgtca
      181 tcacgtcatt ttcatgccgc ggtgctggtt tctatagtgg tgacgtcttg ctgtggatta
      241 tttttcggtg atgttcattt tagcggcgtc tattccattc cgcctgatat tagcggcgtc
      301 attggtgaag tagatttgag cggcgcgtta acacttgaac tcgccggtat cattttctcc
      361 tttatgctga tcaacctatt tgattcatca ggaacattaa ttggtgtaac tgataaagcg
      421 ggcttaatag atggtaacgg taaattcccc aatatgaata aggcgctgta tgttgatagc
      481 gtcagttcgg tggcgggtgc gtttatcggc acctcgtctg ttactgccta tattgaaagt
      541 acttctggtg tggcagtcgg tggccgcacg gggctgactg cggttgtggt tggcgttatg
      601 ttcctgttgg ttatgttctt ctcaccgctg gtggcgatgg ttcctcctta cgcaaccgcc
      661 ggagcgttaa tctttgttgg cgtgctgatg acttcgagcc tggcgcgcgt taactgggat
      721 gattttaccg aatcggtgcc tgcgtttatt accacggtga tgatgccctt tactttctcg
      781 atcaccgaag ggattgcact cggctttatg tcgtactgca tcatgaaagt gtgcaccggg
      841 cgctggcgcg atctgaacct gtgtgtggtg gtggtcgcag ctctgtttgc actgaagatt
      901 attctggtgg attagtaact ggcgagggtt tccagatatc atgagttctg attacgcagg
      961 agaactcatg atctggataa tgctcgccac gctggcggta gtgtttgtgg ttggttttcg
     1021 ggtgctgaca tccggggcca gaaaagcgat tcgccgtctc agtgatcggc tgaatatcga
     1081 tgtcgtaccc gtggagtcga tggtcgatca aatgggaaag tcagccggtg acgaattttt
     1141 acgttatttg catcgcccgg atgagtcgca cctgcaaaac gccgcgcagg tgttgctcat
     1201 ctggcaaatt gtcattgtcg atggtagcga acagaacctg ctgcaatggc atcggatttt
     1261 acaaaaagcc cgtcttgccg cgccgattac cgatgctcaa gtcaggctgg cgctgggttt
     1321 tctgcgcgaa accgaacctg aaatgcagga tattaatgct tttcagatgc gctataacgc
     1381 gttctttcag cctgccgagg gcgttcactg gctgcattaa aaccgcttcg tcttatcagc
     1441 ctacagaccg cgcctgacct gtcgggtgga taagacgttc acgccgcatc cggtaatctt
     1501 tgccctgttc cgtttcgatc ttaactccag ctcaagcaac accgtaaaag ttgcaaaaac
     1561 gttaaatgcg tcacacattt caatgttaaa gtggtcggct tttcccctga aacatgccac
     1621 gggtaacacc atgagtgaat ttattgccga aaaccgcggc gcggatgcca tcacccgacc
     1681 gaactggtca gccgttttct cggtggcgtt ttgtgtcgcc tgtctgatta tcgttgagtt
     1741 tttgcccgtc agtttgttga cgccaatggc ccaggattta ggcatttcgg aaggggttgc
     1801 cgggcaatcg gtgaccgtga ccgcctttgt ggcaatgttt gccagtttgt ttattaccca
     1861 gacaattcag gctactgacc gctgctacgt tgttattttg tttgccgttt tgctgacgct
     1921 ctcctgcttg ctggtttcct ttgctaactc attcagtttg cttttaatcg gtcgtgcctg
     1981 tctggggctg gcgctgggcg ggttctgggc gatgtcggcg tcgctgacca tgcgtctggt
     2041 gccgccgcgt acggtgccga aggcgctgtc ggtgatcttc ggcgcggttt ctattgcgct
     2101 ggtgattgcc gcgccgttgg gcagtttttt aggcgagctt atcggttggc gcaatgtctt
     2161 taatgcggcg gcggtgatgg gcgtgctgtg tattttctgg attatcaaat cattgccttc
     2221 actgccaggc gaaccctcgc atcagaaaca aaatactttc cgcttattac aacgtccggg
     2281 tgtgatggca gggatgatcg ccatcttcat gtctttcgcc gggcagtttg ctttcttcac
     2341 gtatattcgc ccggtgtata tgaacctggc gggattcggc gtggatggct taacgctggt
     2401 gctgttgagt tttggtatcg ccagctttat tggtacgtcg ctttcgtcgt tcattcttaa
     2461 acgttcggta aaactggcct tagcaggcgc gccgttaata ctggctgtga gtgcgttggt
     2521 actgacgttg tggggaagcg ataaaatcgt tgctaccggc gtggcgatta tctgggggct
     2581 aacttttgca ttggttcccg tcggctggtc aacgtggatc acccgctcgc tggccgatca
     2641 ggcagaaaaa gccgggtcta ttcaggtggc ggttattcag cttgctaata cctgtggcgc
     2701 ggcaatcggc ggttatgcgc tggataatat tggtctgact tcgccgttga tgttgtccgg
     2761 cacattgatg ttgctgactg cattgttggt tactgcaaag gtgaaaatga agaaatcctg
     2821 atatcaggta aagaagcgtt ggtcagtagt tgttgctgtt cttacatatc cgggcattct
     2881 accttaccca acgcttccca ataattgctc tgattattac gttccatcgc cacgatggcc
     2941 tcgcgtatat gttgtttatt gttttcatct gacaacatta acttttccca cgcttcatct
     3001 gataatgacg cctgtggcgt gcaaatttta cgtaaatctt gtaataccgt ggttttctct
     3061 tttgcggact gcagtgagct aaaaggcgac tcggcgtata cgatgcctgg catagcgaaa
     3121 aaagtgaaaa taagcagtag tgtcgttggt ttcatggatt caacctttta caaatgtgta
     3181 tcgaatgaag cataagtgta gtttgctttc atcgctctca tcattctgta cgaaacgcgc
     3241 ttgcgtggtc agtaagaagt gccagacttt atattccact tttattcctt tttattctta
     3301 tcgatagcgt ttcgtttttt aaaccgcagc gaccttaccg ctatagtcag gtaatcatta
     3361 ataaaaggat aaaaaaatga aactgacaac acatcatcta cggacagggg ccgcattatt
     3421 gctggccgga attctgctgg caggttgcga ccagagtagc agcgatgcaa aacacattaa
     3481 agttggcgtt ataaatggcg cagaacaaga tgtcgcggaa gtcgctaaaa aggtggcaaa
     3541 agagaaatat ggcctcgatg ttgaactggt aggatttagc ggttcgttgc taccgaacga
     3601 tgcgacaaat catggcgaac ttgatgcgaa tgtcttccag catcgaccct ttcttgaaca
     3661 agataatcag gcgcatggtt ataaactggt agcggtggga aatacgtttg tcttccctat
     3721 ggcgggttat tccaaaaaaa tcacaaccgt ggcgcaaata aaagaaggcg cgacagtggc
     3781 gatccccaac gacccgacca accttggtcg cgcactttta ctgttgcaaa aagagaagct
     3841 aattactctg aaagagggaa aagggttatt acataccgcg ctggatataa ccgataatcc
     3901 tcgtcatttg cagattatgg aactcgaggg ggcgcagctt ccgcgcgtac tggatgatcc
     3961 caaagttgat gtagcgatta tcagcaccac ttacattcag cagacagggc tttctccggt
     4021 gcacgacagc gtatttattg aagataagaa ttcgccgtat gtgaatattt tggtggcacg
     4081 ggaagataat aagaatgcag aaaacgtgaa ggaatttctg caatcttatc aatcacccga
     4141 agtcgctaaa gcggcagaaa ccatttttaa cggtggcgcg gtgcctggct ggtaattctc
     4201 actcatgccg cgccggacga tttatttttc tggccggcga cgggaatcca ttgaaatcaa
     4261 aatcaccgat gacagaatca gcagcgttcc cagccagtcc ggtaatgtga acgtgatccc
     4321 cagcaacagc aaagagagta gcgcgctact taatggttct gcacagctca aaatgctggc
     4381 ttttggaccg ccaattaatt gtgctccttt caggtacaga ctaaatgtca gggacgtacc
     4441 aatgaccacc aaataaaaaa acgccagaat caaactgccg ttaacgacaa agtttgttcc
     4501 ttgtctggca taaaaaggca acagaatcag accgccaatc agcatactcc agccgacgac
     4561 tggtaacgtg ccatagcggg caattagcgt tgagggatag gtggtataga atgcagcagc
     4621 aaatgccgag gcaatgcccc agaacaacgc ggcaggagag agcgataatg acgtcggatt
     4681 accgtgtgtc accaataaaa aagtcccgac cagcgatgtc aaaatagcgc agaaaaccag
     4741 aatgcccggg cgcgatttac gcaccagtga gaaccaggcg acgataatcg tcggtgagag
     4801 gaattgcagc accgttgccg tggctgcgtt cgatttttcg atggttagca aaaaagtgag
     4861 ctgtacagtt agcgcgccaa ccacggaaaa aatcagcagg ctaatggcat ctttatgatt
     4921 gttaataata gaaaagattt tatcgccatg aacaaatgac agcgtcagta gaatcaaacc
     4981 ggcgaatatc aaacgcgtca tagtcaaaaa ctgcgacgac atctggcttt gctccatgat
     5041 gtattgcgcg cagacccctg aacttcccca caacacggcg gcaatcagaa cgttcagcat
     5101 cccctttctg gtggaaccca tttttccctc tgcatactgt tttgtcgttt atgtatgcag
     5161 gcatgatagc aaaaatggcg aggatggctc tgtttgcgcc acgccgattt ttcttagccc
     5221 agactggtga gctaaatatc tttaataaac agcaggcaaa tcatcgcaat acccaacatc
     5281 cctatcgcca gccagaacaa cgcatggtag ctccagattt caaccataat tccgtcaaca
     5341 gagccggcga taatccagcc gacgcgtgaa gtatttgcat ataacgttgt cgcagagcct
     5401 attttttcag gcattaagtc ctgaaaataa agcatgccg
//
LOCUS       DAJVUJ010000217         5340 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.217, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000217 DAJVUJ010000000
VERSION     DAJVUJ010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5340
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.217"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(891..1832)
                     /gene="fabY"
                     /locus_tag="OQQ48_003914"
                     /gene_synonym="yiiD"
     CDS             complement(891..1832)
                     /gene="fabY"
                     /locus_tag="OQQ48_003914"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HCS1954496.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(1877..2314)
                     /gene="dtd"
                     /locus_tag="OQQ48_003915"
     CDS             complement(1877..2314)
                     /gene="dtd"
                     /locus_tag="OQQ48_003915"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCS1954497.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(2311..3184)
                     /locus_tag="OQQ48_003916"
                     /pseudo
     CDS             complement(2311..3184)
                     /locus_tag="OQQ48_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
     gene            complement(3178..3777)
                     /gene="yihX"
                     /locus_tag="OQQ48_003917"
     CDS             complement(3178..3777)
                     /gene="yihX"
                     /locus_tag="OQQ48_003917"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HCS1954498.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRVPLATLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(3918..4805)
                     /locus_tag="OQQ48_003918"
     CDS             complement(3918..4805)
                     /locus_tag="OQQ48_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HCS1954499.1"
                     /translation="MKTERKNEHYLALQQAFDAPWPGPVGELVTLEKGNIHLQIYPHD
                     GARITSLKAFGSEVLRQWQPQRRAFQYGCFPMVPWAGRLGNATLNAGGQCYSLPANKP
                     PHALHGMACYSTWEIIDKTADSLTLRMPLASPWPWQGEVIQTFLLENDALVLQLEVHS
                     YADTFPASAGWHPWFAKKLTPQNTESLQVLFDADWQEEAGSDELPTGNRISPQAGPWD
                     DCFGFYNGVKVKLLWPGKLAMTMISSANSLVVFDKQPDATCINPLTQAPNAINLTPEL
                     VTPDRPLVIETRWQFTPES"
     gene            complement(4795..>5340)
                     /locus_tag="OQQ48_003919"
     CDS             complement(4795..>5340)
                     /locus_tag="OQQ48_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1954500.1"
                     /translation="FGIVYISSQLVLSRDIPLTGMLIASAIGIFSCPLVGWLSDRIGQ
                     KSLYLSGAGFCVLFAFPFFLLLDSKSTLIIWCSMILGYNLGPTMMFAVQPTLFTRMFG
                     TKVRYTGLSFAYQFSAILGGLSPLIASSLLALGGGKPWYVALFLFAVSVLSFVCVWLI
                     EPTDEQETASYRYIREQSHEN"
BASE COUNT         1442 a         1346 c         1253 g         1299 t
ORIGIN      
        1 tctgataaat gaagaaaaac cgtattcatc gccattacca ccaccttacc tatattactc
       61 attaatgtat gtgcgaaaat tacgccggaa aatcattaaa aaaagcggat ttttgcatgt
      121 tgttttgttt ttgcgaggcg ttttccagaa ataataagag tgttgcagtt gtttcattta
      181 ttactcaaaa atggtttagt tatcgcccag gatgaattat gggtgctatg aatatattat
      241 attcagagat atttatttgc ataatctgaa tggtgtagcc gaaaaacgtc agcatcctaa
      301 cagcaccagg atttaaggtg aaattaatct ttcatcacag accggtcagt actgccacca
      361 accggtcttt acttgctcat cgttataatt ttgttggtaa cagttcggga aagttgtttt
      421 tctaaattaa caacagaata actttcttgt ttaatgagat taatattggc aaccattata
      481 gcctccttcc cacggtttaa gagcaaacat acaacataat gattcataac atacagagca
      541 gtaaatgaat gatactgaca atgatgccag cccattatct ttgcataatg tttgtgagct
      601 atagatttta aaaatattta tgaggaaagt atggcaccag aggaggtttt gactatcaaa
      661 aatcgctcaa acctgaacta agatgatgag ttaagcatta tctgcgataa ttgccactca
      721 gctctacaaa taatatttaa atgctcctga acagtgatgt agcaagacac gtatggtgcc
      781 aacgaagaat aaaaatttat agagttctta ttcaatatta ccggcataga cagggaatga
      841 tgtaaatgac cggaatgaat gagagaggca gcaccgcccc tctccataag ctactcttct
      901 tcgttcccgc cctcttcata cgggccaaat ggcttcgcgg gcagaacgat atacgtgcct
      961 tcaaacactg cacccggcgt ctcgtcgcca aagatttcaa cttgcatctg cacccgtgct
     1021 tttcgtccgc gcgccagacg gtcgagatcg ccgcttaagg caccaaggtc ggctaccgca
     1081 tgaggtttac cgctaatcgg cttgctgtag cggatatgcg catcggccag aataatcgtt
     1141 ccgccgaggt ggcgttcgcg cagcatcagc cagataagcc cccaaccggt gagcgttgcc
     1201 agcgagaata aactcccggc aaacagcgta tggtgcggat tctgattacc ggtttccggc
     1261 atggtggtga taaatttttg cccggtatat tgctgaatgc gcacgcccat tttttcacta
     1321 agcgggatgt gttcgtacca cgcctgttgc agctgcgcgc accagtcgcc gcgatgcaaa
     1381 atatcatcca gagtggcaac aggtttaatc atcaaaaaat ggcgaatcgg cgtggtggtt
     1441 ggtgtggtga tttctccctg attaacaaac cccagcttgg cgaaaaactc caccgcgtct
     1501 tcacgggcgc tacaggtcac gcgcttaacg ccttcctgac gcgccaccga ctccagggtc
     1561 atcgccatca acgtccccag ccctttgtcc tgcacgtcgg gatgaacggc cataaagcga
     1621 atggacgctt cattgtcggc attaatatac agtcggccta ccgccaccag attcccctgc
     1681 tcgtcgacga ccatctgatg atgcgccatc gcatcccacg cgtcgcgttc cgaacctttt
     1741 ggttgatgca ggggcttacg caacatttcc cagcgaaact gatagtaacg ctctaactct
     1801 tcttctgttt gtggaacccg aaggtgatac atagcgatac tctctcttgt ttcccgtgac
     1861 aaccctggaa gctggctcat acctgcaacc agaatgtcac ggggccatca ttgaccagcg
     1921 atacctgcat atcggcagcg aagcgtcctg tttgcgtgtt catctcttgc tggcggcagc
     1981 gttcgacgaa atagtcatat aacgcctctg cgcgatccgg tgatgcgcct ttggagaaac
     2041 ttgggcgcat cccccgttcg gtatctgcgg cgagggtaaa ctgggaaacc accagtacac
     2101 tgccgcccgc ctgttgcacg ttgagattca tcttgccttc ggcatcgcta aagatgcggt
     2161 agccgagcac acgctcgcac agacggtttg ctttctgttc gtcgtcatcc ttttcgacac
     2221 ccaataacac caaaagtccc gcgccaattt cgcccgtcac ttctccctcc acggtgacgc
     2281 tggcacgggt tacgcgttga attaatgcaa tcatggttcg tcgtcttctt cttgttcagc
     2341 tgcttgtttt agtttgcggt attccccgag agtgacagta atttccgcgc caagcaagac
     2401 gatacaccat gtccagtaga cccaaacaaa gagaatgggg atcaccgcca gcacaccgta
     2461 aatgagctga tatgacggga acatggtgat ataaagcgcg aaacctttct ttcctgcttc
     2521 gaacaggagt gcggcgacaa acgcgccgac aatcgcgtcg cggttaggta cgcggatggt
     2581 aggaacaatg ctgtacagca accagaagga gatccacgac aacagcaacg gaaaaatacg
     2641 cagcacgttg tcgatgacag tattgagatc gctcgcccag cgcagggaga gcaaatagga
     2701 actgatcgcc agactggccc ctgccagcag cggccccagc gttaaaatca tccagtacac
     2761 ggcgaacgag taaatttttg gtcgcgctcg tttactgcgc cagatggtat tcaacgcgct
     2821 atcgatggag tacatcaaca ataacgcggt gacgatcagc ccgcacgccc caacggcggt
     2881 catcttgttg gaattggcaa caaattgttc gatataccgc tgaataacat cgccagtagc
     2941 aggcagaaag ttggcaaaaa taaagtgacg taactgaatg ctgacgtcgg aaaacatggg
     3001 aaaagcggcg aaaagcgcaa aaacaacggc aaccagcggc actaatgaga gcaacgacca
     3061 cataggcaag gttacctgcc agggttgtca tgttgtcctc atcaatacgt tgccagagta
     3121 gttttagcca ggcccatagt ggacgggtac ggtgcctggc tttgtcctga atggttttta
     3181 gcataacacc ttcgcgaaat agtccgggat ggtggtttta tctttcacca gaatactggt
     3241 aatgcccagc tgattggctc cttctatatt atcggcgtta tcgtcgaaaa agaccgtatc
     3301 gctgggtgaa aaaccttccg cctgcaaaac atgctggtaa attcgtgctt caggtttgcg
     3361 catccccaga tcttgcgaca gatagatatg gtcagcagca tcacgaattt ccgggtattc
     3421 ttccggccag aaggtggtat gcaggcggtt ggtattggaa agcaccacca cgcgatgccc
     3481 ctgctcacgc agtttatgca tgatggcgat cacttccggg cgcagcgcaa caaacaccgc
     3541 ctgccagccg tgagagaact gctcgtagct tagcggtaga gccatctcat gacacagcgc
     3601 ctctgcgaac gcttcgtcgc taatttcccc acgctcatgc tgatgaaacg cctcccccat
     3661 atgaaaactc ttcttaagcg tagccagtgg aacacgcgtt aaatcgctcc aggctcccag
     3721 cacacggtta aagtcgatat cgacaatcac attacctaaa tcaaagatat agagcatttt
     3781 tgcctccttt cgcgccacaa gaaattaact gtagcgggaa aggatgggtt tggctatgtg
     3841 gcaataaggg taatcttgtg atgctgattg cgaaaaagca gagtaatttc actggctttt
     3901 tcgcaataga tgaatggtta agattcaggt gtaaattgcc aacgagtttc gatgaccagc
     3961 ggcctatctg gtgtcaccaa ttccggcgtt agatttattg cgttaggtgc ctgagtcagc
     4021 gggttaatac aggtggcatc gggctgttta tcaaatacca ccagactgtt tgcagaagag
     4081 atcatggtca tcgcgagttt accaggccac agtaacttaa ccttcacccc gttataaaaa
     4141 ccaaaacagt catcccaggg accggcttga ggagagatac gatttccagt tggcagttca
     4201 tcactacccg cttcttcttg ccagtccgca tcaaaaagca cctgcaacga ttctgtattt
     4261 tgcggagtca gctttttcgc aaaccacgga tgccagccag cggaggccgg gaatgtatcg
     4321 gcatatgaat ggacttccag ttgcaaaacc agcgcatcgt tctccagcaa aaaagtctga
     4381 atcacttccc cttgccaggg ccacggtgaa gccaggggca tacgtaaagt cagggaatct
     4441 gctgttttgt cgatgatttc ccacgttgag taacaagcca taccatgcaa cgcatgtggt
     4501 ggtttattgg cgggtaaaga ataacattgc ccaccagcat tcagcgtcgc attccccagt
     4561 cgccctgccc acggcaccat tgggaaacag ccatactgaa aagctctgcg ctgcggctgc
     4621 cattggcgta atacttcaga accaaatgcc tttaacgagg ttatcctggc accatcatgc
     4681 ggataaatct gcaggtgaat attgcctttt tccagcgtca ctaactctcc tacaggccca
     4741 ggccatggcg cgtcaaacgc ctgttgtagc gccaggtaat gctcgttttt tcgttcagtt
     4801 ttcatgagat tgttccctga tgtagcggta agaagcggtt tcttgttcat cggtcggctc
     4861 aatcagccag acgcaaacaa aggaaagaac tgagacagca aataaaaaca gagcgacata
     4921 ccacggtttt cctcccccta gcgccagcaa acttgaggcg ataagggggc ttagtccgcc
     4981 aagaatcgcg gaaaattgat aagcaaaaga aaggccggta tatcgtactt tggtaccgaa
     5041 catgcgggtg aaaagtgttg gctgcacggc aaacatcatc gtcggaccta agttgtagcc
     5101 gaggatcata ctgcaccaga tgatgagcgt acttttgcta tccagtaata agaagaaagg
     5161 aaacgcgaac aggacacaaa atcctgctcc cgacaaatac agactttttt gacctatacg
     5221 atctgataac catccaacca atggacaact gaaaataccg atcgccgagg caatcaacat
     5281 acccgtcagg ggaatatctc gcgacaatac taattggctg gaaatataga caataccaaa
//
LOCUS       DAJVUJ010000218         5209 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.218, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000218 DAJVUJ010000000
VERSION     DAJVUJ010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5209
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.218"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..117
                     /locus_tag="OQQ48_003920"
     CDS             <1..117
                     /locus_tag="OQQ48_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3829 domain-containing protein"
                     /protein_id="HCS1954501.1"
                     /translation="DWDKEQLQDANSSWMVEDSFPRALREYNEMVDDYNSLR"
     gene            complement(167..1915)
                     /locus_tag="OQQ48_003921"
     CDS             complement(167..1915)
                     /locus_tag="OQQ48_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HCS1954502.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARISASGSAGYQLEIFERRSFFQLLQKHDNDYRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERACCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GVTRDNLQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGDEIKQIV
                     EQSGMFLGDNAVRTLTGLIEKQHQQAQVISADHVLGLLQRVPDIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDNDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLNCRVIIARSLSELVAIREE
                     IEPLLIINNSHYLLEDAVNNYITVKNIITAAGIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASILCQHPAEVI
                     AGLTGYEAFMELLHKG"
     gene            complement(1915..2985)
                     /locus_tag="OQQ48_003922"
     CDS             complement(1915..2985)
                     /locus_tag="OQQ48_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCS1954503.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGSVAPHALTEKQRQRPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKSCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RDFLTTLTAEKVNAFSQFRQMD"
     gene            complement(2975..4426)
                     /locus_tag="OQQ48_003923"
     CDS             complement(2975..4426)
                     /locus_tag="OQQ48_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HCS1954504.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFTGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEMQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            complement(4437..4883)
                     /locus_tag="OQQ48_003924"
     CDS             complement(4437..4883)
                     /locus_tag="OQQ48_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCS1954505.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHKEFIHQLQQGDTDQVLALLNQTLSS"
BASE COUNT         1319 a         1342 c         1316 g         1232 t
ORIGIN      
        1 gactgggata aagagcaact tcaggacgca aactcaagct ggatggtcga agactctttc
       61 ccgagagcat tacgcgagta caacgaaatg gttgatgact ataatagcct gcgttaaacg
      121 tttttgatga taaatgcagt gtcggatgcg gcgcatctga cacagttcac cccttgtgaa
      181 gtaactccat aaatgcctca tatcctgtta acccggcaat aacctctgcc ggatgctgac
      241 acaatatgct tgccagatag ctaaatattt ttagctcatg acgcgcatcg gcggcggcgc
      301 aggcgatcaa gacatggtta atgacttcgt tattcacctc aactggttgg gcgagggtaa
      361 taaaaaatcc acgaaaacgt cgctcctgtt cgctccagca atgtgggatg gcgaggcgat
      421 taacaatcag gttttcacct tcgccttcgc gggcgatgat gcgttgtgct tcatcggcgg
      481 taatatggtg ttgtgcgact aattgcgcac aaatttgccg ggtaatatgt tgccagcttt
      541 caccgcgtac attcgaataa tgaaaacttc cgggggcaga gaaaaaacgc tccggttgct
      601 ggcgaataaa tgccgtcgcc agaaaatgtt ttatttgttc gataccggca gccgtaatga
      661 tattttttac ggtgatgtaa ttatttaccg cgtcttccag taaataatgg ctattgttaa
      721 tgatcaataa cggctcaatc tcttcgcgaa tggcaacaag ttcgcttaag ctacgggcaa
      781 taattacccg acaatttaaa acatcgcgct caatggcgag ctgattaata gtggcaatcg
      841 cgttctggtc cgagagcaaa atgatcggct ggcgttcgtt ttgatgccgc tccagcgcac
      901 aggcaaaata caaaccgatc agatcgttgt cgaaaagggg aatatcgagc tgttcacgca
      961 gtagcgtaat aaagtgcaga ctcatatcaa aggccgcagg ccaggctgct ttcaggttat
     1021 tcatgctact ttggcggtgc tcagcaatcc acaccggtgc ggcaaggcaa cgtaataaat
     1081 gcccggtaat attctcaacc aactgcgtat caataatatt caatgacgcg atgtctggca
     1141 ccctttgcag caaccccagc acatgatcgg ctgaaattac ctgcgcttgc tgatgctgtt
     1201 tctctatcaa acctgtcagg gttcttacag cgttatcacc aagaaacatg ccgctttgtt
     1261 caacgatttg ttttatctcg tcaccgggat attgcggcca ctcatcggtc agttgattac
     1321 gcagggcgta aatcgccaga atcaggctgg agagataatc accctgcatg agcggccagc
     1381 ggtgttggtt gtcgcaggcc gtggataaat gctgaaggtt gtctcgtgtt acgcccgcca
     1441 gcggaattaa aaacggatct ttgcgcagca agttcgccag caagataacg cgcttctctt
     1501 ctgtctcatc aatgaaatgg cccaaaccgg ggcggctggc caggcaacaa gcgcgttcat
     1561 aacgctgttt taaccggggc agacgctctg cgacccacgt ttctggcaaa ttaagcgccg
     1621 aggcgagttg cgcacgggga gtaaaggtat tcagtaataa cagtgccagc agccgatagt
     1681 cgttatcgtg cttttgcagc aactgaaaaa agctgcggcg ctcgaagatt tccagctgat
     1741 agcccgcact accactggcg gaaatgcggg ctttgccgtt aagggtaaag ttgagatagt
     1801 caatatcacg caggatggtc ctgcctgaaa cgccagtctg ttgcgccagc tcaccaggcg
     1861 tgcgcggctg ttgctccagc agatcgacaa tctttaactg gcgttcgttt agcattaatc
     1921 catctgacgg aactggctaa acgcgttgac tttctccgcg gttaaggtgg tcagaaaatc
     1981 ccgtatcaac gtgagcagag catcgtaatc ggcttttgaa atcataccgc tgttagcgtg
     2041 cagatagcgg gttggcaggc acagcgcaac aaccggacgc ccgccgccca tcacgttgta
     2101 gcgcccgcca tccgtcgcac cggttttcat ggtggaaaat tgcagcggta aatcgttatg
     2161 tgcggcacag ctttttaacg ctgctaccag tttctggttg gggaagtagc gcttgtcaaa
     2221 cagcatcagc cccggcccct ggcccagttt cagcgggtat ttaatgttat caatgcccgg
     2281 aacatcgcct gcgacagcgg tatccagcac gatcactaca tccggtttaa tgtgttccgc
     2341 cgaggtttgt gccccgcgta gcccaacttc ttcttccacg ctgccgacgc catacagcgt
     2401 aatttcggga ttattcaccg tctgcaatag ttcagccatc attgcgcagc cgatgcggtt
     2461 atccagcgcc ttgccgacca ctttatcttc gccccagcag gcaaaattgg cttccgggct
     2521 aataaaatcg cccatcgcca caccgcgttt ttccacctca tcgcgactgt ttgcgccaat
     2581 atcaatgaac atctcatcaa atgacagcgg ccgttgcctt tgcttttccg ttaacgcatg
     2641 aggcgcgacg gaaccaatca caccagggat tttcactccc ttgtgtgtgc gtatggttac
     2701 ccggtggttg agcatcgact gattccacca gccaccaatg gtggtgaaac gcagaaaacc
     2761 gctctcgtcg atgtgggtga ccataaagcc gacttcatcc atatgcccga caacggcaac
     2821 ttttggacct ttattcccct tacgggcaac aaagctgccc agaccatcaa aggtgatctc
     2881 attaacgcaa ggttccagcg tgtttatcag aatgtcgcga acttcctgtt catcgccgct
     2941 gacggcgctg gcttcgcaca actgttgcag taactcaatg ttcatggaca agctcctgtt
     3001 gcgcggcttt cgctttgcgg cgtaaccaca atcctttcag gacgataatg aatgaaatgt
     3061 tcagcgcgag gccaatcgcc agcaccagat aaaacgaacc caccggcgac atcaggccga
     3121 tcagcgggtc aaagataccc aaaccaggtg ccagacgttt gattccgaaa gcgatcacca
     3181 gcacgccggt aatgccaccc gccagcgtat tggcggtaat catcggcaac ggagctgcca
     3241 gagcgtaagg tatagccggt tcggttgcga ctgtggcacc gaccacaatg gcactactgg
     3301 cagcctcttt ttcctgtcgg gtaaacagtt tgggcgcaat gaacgtcgca agacccgccg
     3361 caacgggcgg catcaacgcg acaacgccga cgatggcgta ccagtcataa atgtgttttt
     3421 ccagcagtga gaagcagaag aaccaggcgg ttttattgat ggggccgccc atatcgaacg
     3481 ccagcatcgc accgaccaga aacgctgctg caaacttcat tgatggagga atggtattca
     3541 ggaagtggag cagcccgccc atgaggtcgg acatcaccgg cccgataacg tagtaagtga
     3601 gcacgccgaa aaccagcaga gtaacgaacg ggatcagcat tgaaccgagc agcggttgta
     3661 atgctttgcc aaggcgcact ttgcggaacc agaagacgaa atagccaatc gccagcccca
     3721 gcaccaccgc gccgaggaat cccgcacccg actgggtgcc gagcagcgct ttgtcgttcg
     3781 ccagatagca aaccagaaac gccggagcga aagccggttt atcggcaatc gacgaggcaa
     3841 tataagcgcc catgatgggg atcataaagg tgaagccaag gtaaccgatg gattcaacta
     3901 cccaggtaaa cgacggcgcg cctttcgaca tatcggtata cggcaaaccg aactgcacca
     3961 gcatgttggc aagcgccacc aggataccac cgccgatgac aaacggcagc gccgcagaga
     4021 cgcccgccat caggtggctc atcacgctgc cgctttgcat ttcctgtttg ccaagcttca
     4081 cgccactatc tgcggcaaaa agctgcgagt ttgtcggtaa ttcgctgaaa atctggtcga
     4141 tatttttcaa cgcctgggag atggcaatct cataaacttt cttcccggta aaccgcgcgc
     4201 gatcatcacc gctcaggcca cgcccggtag cgagaatgac gtaatcggca gcggcaatct
     4261 cttcgctgga taagcggttt tcaacgccac tggacccttg agtttccact tttatggtat
     4321 ggccgagaga acgggctttc tgttccaggg cttccgccac catgtaggtg tgagcgatcc
     4381 cggcggggca gttggtgatc gcgacaatac gtaaggatga ctccatcgcc acttccttat
     4441 gagctgaggg tttgatttaa caaggcaagc acttggtcgg tatcgccctg ttgcagttga
     4501 tgaataaatt ccttgtgaat aattttgcga cacagtgtgc cgatgatttt gacctgatcc
     4561 tcttcgccgc tttgcggcac gccgaggcag atccagcaat tgacgtcttc gccatcgctg
     4621 gctttccagt caatagcctg cgctttgcgg gcgaataata cgaacggttg tttaacgcag
     4681 gcgcttttac cgtgcggcac ggcgacaccg gaaccaaatc cagtagagtg cattttttcg
     4741 cgcagcagca gggtttgcag gaactggtgt gagtcggtga taaaaccgtt ttgtaacgct
     4801 attgtcgcca actgcttcag aacggaataa gcgccattgc cctgaatatt caggtcaata
     4861 cagcttgcag taagagctgc cattaatcgg actccataaa atatatttta atcaattgta
     4921 atggtggagc gattaagtga tgagaggcga tatgtcggta aacaatgaca atttgagaca
     4981 gagtgaaagg tcagatttgc ggagtaatgc acataatggt tatttaaata aaccacatga
     5041 atcattaatg ggttattcat tatttttgtg atttactcac aatgtatcag ggaaatataa
     5101 cttaccggga gatgtaatat gtttaatttt tcaaatcgaa atttaaaata ttgtgccgga
     5161 ggcatctccg gcacattggg caattacggc aggtaaaaca cttcttgca
//
LOCUS       DAJVUJ010000219         4958 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.219, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000219 DAJVUJ010000000
VERSION     DAJVUJ010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4958
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.219"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(<1..245)
                     /locus_tag="OQQ48_003925"
     CDS             complement(<1..245)
                     /locus_tag="OQQ48_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3029 family protein"
                     /protein_id="HCS1954506.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLA"
     gene            503..1282
                     /gene="deoC"
                     /locus_tag="OQQ48_003926"
     CDS             503..1282
                     /gene="deoC"
                     /locus_tag="OQQ48_003926"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCS1954507.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQTKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            1409..2731
                     /gene="deoA"
                     /locus_tag="OQQ48_003927"
     CDS             1409..2731
                     /gene="deoA"
                     /locus_tag="OQQ48_003927"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HCS1954508.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDARALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            2783..4006
                     /gene="deoB"
                     /locus_tag="OQQ48_003928"
     CDS             2783..4006
                     /gene="deoB"
                     /locus_tag="OQQ48_003928"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCS1954509.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            4086..4805
                     /gene="deoD"
                     /locus_tag="OQQ48_003929"
     CDS             4086..4805
                     /gene="deoD"
                     /locus_tag="OQQ48_003929"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCS1954510.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
BASE COUNT         1194 a         1261 c         1363 g         1140 t
ORIGIN      
        1 ttcgccagaa aacgggcgta atcgggtaag acatagcgcg gtttgtacgg cgcatgacct
       61 tcaaacatat cgcagattac accctcatcc agcgcgcggc gggcttcggc aggaagctgc
      121 ggataaggca gattgttttc tgcttccaga gccagaaaat ggcgcttctg ctccgggcta
      181 agcactgggc tggtgacaat ttgctggcaa cgttgttgca gtgcattttc atgagaagtg
      241 ggcatcttct tttcctttta tgccgaaggt gatgcgccat tgtaagaagt ttcgtgatgt
      301 tcactttgat cctgatgcgt ttgccaccac tgacgcattc atttgaaagt gaattatttg
      361 aaccagatcg cattacagtg atgcaaactt gtaagtagat taccttaatt gtgatgtata
      421 tcgaagtgtg ttgcggagta gatgttagaa tactaacaaa ctcgcaaggt gaattttatt
      481 ggcgacaagc caggagaatg aaatgactga tctgaaagca agcagcctgc gtgcactgaa
      541 attgatggac ctgaccaccc tgaatgacga cgacaccgac gagaaagtaa ttgctctgtg
      601 tcatcagacc aaaactccgg tcggcaatac cgccgctatc tgtatctatc ctcgctttat
      661 cccgattgct cgcaaaacgc tgaaagagca gggcaccccg gaaatccgaa tcgctacggt
      721 aaccaacttc ccacacggta acgatgacat cgaaatcgcg ctggcagaaa cccgtgcggc
      781 aatcgcctac ggtgctgatg aagttgacgt tgtgttcccg taccgcgcgc tgatggcggg
      841 taacgagcag gttggttttg acctggtgaa agcctgtaaa gaggcttgcg cggcagcgaa
      901 tgtactgctg aaagtgatca tcgaaaccgg cgaactgaaa gacgaagcgc tgatccgtaa
      961 agcgtctgaa atctccatca aagcgggtgc tgacttcatc aaaacctcta ccggtaaagt
     1021 ggcagtgaac gcgacgccgg aaagcgcgcg catcatgatg gaagtgatcc gtgatatggg
     1081 cgtagaaaaa accgttggtt tcaaaccggc gggcggcgtg cgtactgcgg aagatgcgca
     1141 gaaatatctc gccatcgcag atgaactgtt cggtgctgac tgggcagatg cgcgtcacta
     1201 ccgctttggt gcttccagcc tgctggcaag cctgttgaaa gcgctgggcc acggtgacgg
     1261 taagagcgcc agcagctact aagcaagatg ctttacgcct gatgcgctgc gcttatcagg
     1321 cctacgagac gtatctaccc gtaggccgga taaggcgtag acgcatccgg caaaagccgc
     1381 ctcatactct tttcctcggg aggttacctt gtttctcgca caagaaatta ttcgtaaaaa
     1441 acgtgatggt catgcgctga gcgatgaaga aattcgtttc tttatcaacg gcattcgcga
     1501 caacactatc tccgaagggc agattgccgc cctcgcgatg accattttct tccacgatat
     1561 gacaatgcct gagcgtgtct cgctgaccat ggcgatgcga gattcaggaa ccgttctcga
     1621 ctggaaaagc ctgcatctga atggcccgat tgttgataaa cattccaccg gcggcgtcgg
     1681 cgatgtcact tcgctgatgt tggggccgat ggtcgcagcc tgcggcggct atattccgat
     1741 gatctccggt cgcggcctcg gtcatactgg cggtacgctc gacaaactgg aatccatccc
     1801 tggcttcgac attttcccgg atgacaaccg tttccgcgaa attattaaag acgtcggcgt
     1861 ggcgattatc ggtcagacca gctcactggc tccggcggat aaacgtttct acgcgacccg
     1921 tgatattacc gcaaccgtgg actccatccc gctgatcact gcctcgatcc tggcgaagaa
     1981 actggcggaa ggtctggatg cgctggtgat ggacgtgaaa gtgggtagcg gcgcgtttat
     2041 gccgacctac gaactctctg aagcccttgc cgaagcgatt gttggcgtgg ctaacggcgc
     2101 tggcgtgcgc accaccgcgc tgctcaccga catgaatcag gtactggcct ccagtgcagg
     2161 taacgcggtt gaagttcgtg aagcggtgca gttcctgacg ggtgaatatc gtaacccgcg
     2221 tctgtttgat gtcacgatgg cgctgtgcgt ggagatgctg atctccggca aactggcgaa
     2281 agatgacgcc gaagcgcgcg cgaaattgca ggcggtgctg gacaacggta aagcggcaga
     2341 agtctttggt cgtatggtag cggcacaaaa aggcccgacc gacttcgttg agaactacgc
     2401 gaagtatctg ccgacagcga tgctgacgaa agcagtctat gctgataccg aaggttttgt
     2461 cagtgaaatg gatgcccgcg cgctggggat ggcagtggtt gcaatgggcg gcggtcgtcg
     2521 tcaggcatct gacaccatcg attacagcgt cggctttact gatatggcgc gtctgggcga
     2581 ccaagtagac ggtcagcgtc cgctggcggt tatccacgcg aaagacgaaa acagctggca
     2641 ggaagcggcg aaagcggtga aagcggcaat taaacttgcc gataaagcac cggaaagcac
     2701 accaactgtc tatcgccgta tcagcgaata acggtatact gatctgatca tttaaatttg
     2761 aagcactgag tacggagaac atatgaaacg tgcatttatt atggtgctgg actcattcgg
     2821 catcggcgct acagaagatg cagaacgctt tggtgacgtc ggggctgaca ccctgggtca
     2881 tatcgcagaa gcttgtgcca aaggcgaagc tgataacggt cgtaaaggcc cgctcaatct
     2941 gccaaatctg acccgtctgg ggctggcgaa agcacacgaa ggttctaccg gtttcattcc
     3001 ggcgggaatg gacggcaacg ctgaagttat cggcgcgtac gcatgggcgc acgaaatgtc
     3061 atccggtaaa gataccccgt ctggtcactg ggaaatcgcc ggtgtcccgg ttctgtttga
     3121 gtggggatac ttctccgatc acgaaaacag cttcccgcaa gagctgctgg ataaactggt
     3181 cgaacgcgct aatctgccgg gttacctcgg taactgccac tcttccggta cggtcattct
     3241 ggatcagctg ggcgaagagc acatgaaaac cggcaagccg attttctata cctccgctga
     3301 ctccgtgttc cagattgcct gccacgaaga aacctttggc ctggataaac tctacgaact
     3361 gtgcgaaatc gcccgtgaag agctgaccaa cggcggctac aacatcggtc gtgttatcgc
     3421 tcgtccgttt atcggtgaca aagccggtaa cttccagcgt accggtaacc gtcacgactt
     3481 ggctgttgag ccgccagcac cgaccgtgct gcagaaactg gttgatgaaa aacacggtca
     3541 ggtggtttct gtcggtaaaa ttgcagacat ctacgccaac tgcggtatca ccaagaaagt
     3601 gaaagcaact ggcctggacg cgctgtttga cgccaccatc aaagagatga aagaagcggg
     3661 tgataacacc atcgtcttca ccaacttcgt tgacttcgac tcttcctggg gccaccgtcg
     3721 cgacgtcgcc ggttatgctg cgggtctgga gctgttcgac cgccgtctgc cggagctgat
     3781 gtctttgctg cgcgatgacg acatcctgat cctcactgct gaccacggtt gtgatccgac
     3841 ctggaccggt actgaccaca cgcgtgaaca cattccggta ctggtttacg gcccgaaagt
     3901 aaaaccgggt tcactgggtc accgtgaaac cttcgcggat atcggccaga ctctggcaaa
     3961 atattttggt acttctgata tggaatatgg caaagccatg ttctgataat gggtgcggct
     4021 tgatgccctc accccggccc tctcccacag ggagagggtg aaaacatgtt gataaggata
     4081 aaacaatggc taccccacac attaatgcag aaatgggcga tttcgctgac gtagttttga
     4141 tgccaggcga cccgctgcgt gcgaagtata ttgctgaaac tttccttgaa gatgcccgtg
     4201 aagtgaacaa cgttcgcggt atgctgggct tcaccggtac ttacaaaggc cgcaaaattt
     4261 ccgtaatggg tcacggtatg ggtatcccgt cctgctccat ctacaccaaa gaactgatca
     4321 ccgatttcgg cgtgaagaaa attatccgcg tgggttcctg tggcgcagtt ctgccgcacg
     4381 taaaactgcg cgacgtcgtt atcggtatgg gtgcctgcac cgattccaaa gttaaccgca
     4441 tccgttttaa agatcatgac tttgccgcta tcgctgactt tgacatggtg cgtaacgcgg
     4501 tagacgcggc taaagcactg ggcattgatg ctcgcgttgg taacctgttc tccgctgacc
     4561 tgttctactc tccggacggc gaaatgttcg acgtgatgga aaaatacggc atcctcggcg
     4621 tggaaatgga agcggctggt atctacggcg tcgctgcaga atttggcgcg aaagccctga
     4681 ccatctgtac cgtgtctgac cacatccgca ctcacgagca gaccactgcc gctgagcgtc
     4741 agaccacctt caacgacatg atcaaaatcg cactggaatc cgttctgctg ggcgataaag
     4801 agtaattgtg tttcgctgaa aggcgattgt cgtgtgaagc cggagtggga gactgctccg
     4861 gctttttagt atctattcat caatagcaac tgatttatag atttactatt atttttcata
     4921 tggatagaaa ataaaatcta ttcaagtatc tattcacg
//
LOCUS       DAJVUJ010000220         4855 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.222, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000220 DAJVUJ010000000
VERSION     DAJVUJ010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4855
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.222"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(<1..1250)
                     /locus_tag="OQQ48_003930"
     CDS             complement(<1..1250)
                     /locus_tag="OQQ48_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AGCS family amino acid carrier protein"
                     /protein_id="HCS1954511.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPRAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFALLAITRGLHGVARLMQGFVPLMAIIWVLT
                     SMVICVINIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNDTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNTPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMA"
     gene            1529..2482
                     /gene="tal"
                     /locus_tag="OQQ48_003931"
     CDS             1529..2482
                     /gene="tal"
                     /locus_tag="OQQ48_003931"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCS1954512.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPTLLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            2597..3184
                     /gene="mog"
                     /locus_tag="OQQ48_003932"
     CDS             2597..3184
                     /gene="mog"
                     /locus_tag="OQQ48_003932"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HCS1954513.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSVLTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(3219..3785)
                     /gene="satP"
                     /locus_tag="OQQ48_003933"
     CDS             complement(3219..3785)
                     /gene="satP"
                     /locus_tag="OQQ48_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HCS1954514.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YSGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(3934..4647)
                     /gene="msyB"
                     /locus_tag="OQQ48_003934"
     CDS             complement(3934..4647)
                     /gene="msyB"
                     /locus_tag="OQQ48_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HCS1954515.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAFDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            complement(4673..>4855)
                     /locus_tag="OQQ48_003935"
     CDS             complement(4673..>4855)
                     /locus_tag="OQQ48_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HCS1954516.1"
                     /translation="AARSASQSLNIPSEIKEGQTTDWININSDNDNKRCVSKITFSGH
                     TVNSSDMATLKIIGDD"
BASE COUNT         1275 a         1251 c         1230 g         1099 t
ORIGIN      
        1 gccattatga tatctgccag ttgccacatc agcggaaggc ttagcaaggt gccgccgatg
       61 accgttgcga aggtgcagat ccgcaaacac cagatcgctt taggggtgtt caggcgtaaa
      121 aagaagagat tgttttcggc ataaatgtag ttggcaacga tggagctgaa ggcaaacaga
      181 ataaccacga gggtaacaaa ctcagcaccc caggaaccca ttagcacccg catcgccttc
      241 tggataagct gaataccttc cagcggcatg taggttgtgt cgttacccgc cagtaatatc
      301 agcatggcgc ttgccgtaca gatgaccagg gtgtcgataa aaatgccaat catctggaca
      361 atcccttgcg ctgccggatg cggaggccag gacgccgctg ccgctgccgc gtttggcgtc
      421 gaacccattc ccgcctcatt ggaaaacata ctgcgctgaa aaccgttagt aatcgcctgg
      481 cttaaggtat atcccgccgc gccgcctgcc gcttcctgcc agccaaaagc actctcaaaa
      541 atagaccaaa tgacgtgggg aagttgcccg atatttatta cgcaaatcac catgctggtc
      601 agcacccaga ttatcgccat caacgggaca aacccctgca tgagccgggc gacgccatga
      661 agaccgcgag tgattgccag cagagcaaag acagcgagaa taatgcctgt caccagcggg
      721 ggaaaatcaa aagaaaaact cagggctcgg gcaacggcgt tcgcctgaac tccgctgaaa
      781 attatgccgt aggcgatgag caaaaagacg gcgaacagaa cgcccatcca acgcatcccc
      841 agcccgcgcg ccatatacca tgccggtccg ccacgaaact gcccattgac gtcacgttct
      901 ttataaagtt gtgcaaggga acattcggca aacgaggtcg ccatgccgat aaacgcggca
      961 acccacatcc aaaagacggc tctaggtcca ccggcggtaa tagccagcgc aacgccggcc
     1021 aggttgccgc tacccacgcg cgccgcaaga ctggtacaca atgactgaaa tgaggttaaa
     1081 ccgcctggct gtggatgaat gctattttta agacttttgc caaactggcg gatgtagcga
     1141 aactgcacaa atccggtgcg aaaagtgaac caacaacctg cgccgaagag caggtaaatc
     1201 attaccgatc cccaaaggac gctgttaata aaggagaaaa aatctggcat gcatatccct
     1261 cttattgccg gtcgcgatga ctttcctgtg taaacgttac caattgttta agaagtatat
     1321 acgctacgag gtacttgata acttctgcgt agcatacatg aggttttgta taaaaatggc
     1381 gggcgatatc aacgcagtgt cagaaatccg aaacagtctc gcctggcgat aaccgtcttg
     1441 tcggcggttg cgctgacgtt gcgtcgtgat atcatcaggg cagaccggtt acatccccct
     1501 aacaagctgt ttaaagagaa atactatcat gacggacaaa ttgacctccc ttcgtcagta
     1561 caccaccgta gtggccgaca ctggggacat cgcggcaatg aagctgtatc aaccgcagga
     1621 tgccacaacc aacccttctc tcattcttaa cgcagcgcag attccggaat accgtaagtt
     1681 gattgatgat gctgtcgcct gggcgaaaca gcagagcaac gatcgcgcgc agcagatcgt
     1741 ggacgcgacc gacaaactgg cagtaaatat tggtctggaa atcctgaaac tggttccggg
     1801 tcgtatctca actgaagttg atgcgcgtct ttcctatgac accgaagcgt caattgcgaa
     1861 agcaaaacgc ctgatcaaac tctacaacga tgctggtatt agcaacgatc gtattctgat
     1921 caaactggct tctacctggc agggtatccg tgctgcagaa cagctggaaa aagaaggcat
     1981 caactgtaac ctgaccctgc tgttctcctt cgctcaggct cgtgcttgtg cggaagcagg
     2041 cgtgttcctg atctcgccat ttgttggccg tattcttgac tggtacaaag cgaatactga
     2101 taagaaagag tacgctccgg cagaagatcc gggcgtggtt tctgtatctg aaatctacca
     2161 gtactacaaa gagcacggtt atgaaaccgt ggttatgggc gcaagcttcc gtaacatcgg
     2221 cgaaattctg gaacttgcag gctgcgaccg tcttaccatc gcacctacac tgctgaaaga
     2281 gctggctgag agcgaagggg ctatcgaacg taaactgtct tacaccggcg aagtgaaagc
     2341 gcgtccggcg cgtatcactg agtccgagtt cctgtggcag cacaaccagg atccaatggc
     2401 agtagataaa ctggcggaag gtatccgtaa gtttgctatt gaccaggaaa aactggaaaa
     2461 aatgatcggc gatctgctgt aatcattctt agcgtgaccg ggaagtcggt cacgctacct
     2521 cttctgaagc ctgtctgtca ctcccttcgc agtgtatcat tctgtttaac gagactgttt
     2581 aaacggaaaa atcttgatga atactttacg tattggctta gtttccatct ctgatcgcgc
     2641 atccagcggc gtttatcagg ataaaggcat ccctgcgctg gaagaatggc tgacatcggt
     2701 gctaaccacg ccgtttgaac tggaaacccg cttaatcccc gatgagcagg cgatcatcga
     2761 gcaaacgttg tgtgagctag tggatgaaat gagttgccat ctggtgctca ccacgggcgg
     2821 aactggcccg gcgcgtcgtg acgtaacgcc cgatgcgacg ctggcagtag cggaccgcga
     2881 gatgcctggc tttggtgaac agatgcgcca gataagcctg cattttgtac caactgcgat
     2941 cctttcgcgt caggttggtg tgattcgcaa acaggcgctg atccttaact tacccggtca
     3001 gccgaagtct attaaagaga cgctggaagg tgtgaaggac gctgcgggta acgttgtggt
     3061 acacggtatt tttgccagcg taccgtactg cattcagttg ctggaagggc catacgttga
     3121 aacggcacca gaagtggttg ctgcattcag accgaagagt gcaagacgcg acgttagcga
     3181 ataaaaaaat ccccccgagc ggggggatct caaaacaatt agtgggattc accaatcggc
     3241 agaacggtgc gaccaaactg ctcgttcagt acttcaccca tcgccagata gattgcactg
     3301 gcaccacaga tcagaccaat ccagccggca aagtggatga ttgcggcgtt accggcaatg
     3361 ttaccgatcg ccagcagggc aaacagcaca gtcaggctaa agaaaacgaa ttgcagaacg
     3421 cgtgcgcctt tcagcgtgcc gaagaacata aacagcgtaa atacgcccca cagacccagg
     3481 tagacaccaa ggaactgtgc atttggcgca tcggtcagac ccagtttcgg catcagcaga
     3541 atcgcaacca gcgtcagcca gaaagaaccg taagaggtga atgcggttaa accgaaagtg
     3601 ttgccttttt tgtactccag cagaccagca aaaatttgcg cgatgccgct gtagaaaatg
     3661 cccatggcaa gaataatacc gtccagagcg aaataaccca cgttgtgcag gttaagcaga
     3721 atggtggtca tgccgaagcc catcaggccc agcggtgccg gattagccaa cttagtgttg
     3781 cccataattc ctcaaaaatc atcatcgaat gaatggtgaa ataatttccc tgaataactg
     3841 tagtgttttc agggcgcggc ataataatca gccagtgggg cagtgtctac gatcttttga
     3901 ggggaaaatg aaaattttcc ccggtttccg gtatcagacc tgagtggcgc taaccatccg
     3961 gcgcaggcag gcgatttgca gtacggctgg aatcgtcacg cgataggcgc tgccgctgac
     4021 cgctttaacc ccatttagtg ccgcacctac agggcctccc agccccgcgc cgcgcagcaa
     4081 accatgccca agtacgctca ttgctgcgtg ggtgcgtaaa atgcgggtca gttggctgga
     4141 aagcaaatgc gacacgcctt ttgccaataa tttgtctttc atcagcagcg gcagcagctc
     4201 ttccagctca ttcaccctcg catcgaacgc gtgcagaaac tcctgcttat gttcctcgtc
     4261 cattttcttc caggtattac gcagaaattg ttccagtaac tgttgctcaa tttcaaacgt
     4321 agacatctct ttgtcggctt tcagcttcaa tcgttttgaa acatcgagca aaatggcccg
     4381 atacaattta ccgtgtccgc gcagtttgtt ggcgatacta tcgccaccaa aatgctgtaa
     4441 ttctccagca atcagctgcc agttgcggcg atgttgctcg ggatgccctt ccatcgattt
     4501 aaacagttcg ttgcgcatca gtacgctgga gaggcgagtt ttgccttttt cattatgggt
     4561 gagcaatcgg gcgaaatttg ccaactgttc ctcactacaa tgctgaagaa aatccagatc
     4621 tgaatcattc aggtaattaa cattcatttt ttgtggcttc tatattctgg cgttagtcgt
     4681 cgccgataat tttcagcgtg gccatatccg atgagttcac cgtatgaccg gaaaaggtga
     4741 tttttgagac gcagcgttta ttgtcgttat cactgttaat gttgatccag tcagtggttt
     4801 gcccttcttt tatttctgaa ggaatattca gactctgact ggcgctacgg gcggc
//
LOCUS       DAJVUJ010000221         4825 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.223, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000221 DAJVUJ010000000
VERSION     DAJVUJ010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4825
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.223"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(<1..4051)
                     /locus_tag="OQQ48_003936"
     CDS             complement(<1..4051)
                     /locus_tag="OQQ48_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HCS1954517.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDGWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASEEN
                     AIALGRSSVASGTDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMA
                     MGFGSLADKVNTIALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALG
                     TASNATGDKSLALGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAI
                     KSDSYDAINGSQLYAISDSVAKRLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAV
                     LDENTLQWDQTKGKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVE
                     ANTANIATNTSNIATNTANIATNTTNITNLTDSVGDLQADALLWNETKKAFSAAHGQD
                     TTSKITNVKDADLTADSTDAVNGSQLKTTNDAVATNTTNIANNTSNIATNTTNISNLT
                     ETVTNLGEDALKWDKDNGVFTAAHGTETTSKITNVKDGDLTTGSTDAVNGSQLKTTND
                     AVATNTTNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGNNTASKITNILDG
                     TVTATSSDAINGSQLYDLSSNIATYFGGNASVNTDGVFTGPTYKIGETNYYNVGDALA
                     AINSSFSTSLGDALLWDATAGKFSAKHGTNGDASVITDVADGEISDSSSDAVNGSQLH
                     GVSSYVVDALGGGAEVNADGTITAPTYTIANADYDNVGDALNAIDTTLDDALLWDADA
                     GENGAFSAAHGKDKTASVITNVANGAISAASRDAINGSQLYTTNKYIADALDGDAEVN
                     ADGTITAPTYTIANAEYNNVGDALDALDDNALLWDETANGGAGAYNASHDGKASIITN
                     VANGSISEDSTDAVNGSQLNATNMMIEQNTQIINQLAGNTDATYIQENGAGINYVRTN
                     DDGLAFNDASAQGVGATAIGYNSVAKGDSSVAIGQGSYSDVDTGIALGSSSVSSRVIA
                     KGSRDTSITENGVVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEAHDAVTVRQLQ
                     NAIGAVATTPTKYFHANSTEEDSLAVGTDSLAMGAKTIVNGDKGIGIGYGAYVDANAL
                     NGIAIGSNAQVIHVNSIAIGNGSTTTRGAQTNYTAYNMDAPQNSVGEFSVGSADGQRQ
                     ITNVAAGSADTDAVNVGQLKVTDERVAQNT"
     gene            complement(4095..4778)
                     /locus_tag="OQQ48_003937"
     CDS             complement(4095..4778)
                     /locus_tag="OQQ48_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCS1954518.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IDLLEKQQAQLKSQLETIQKQQTGIISSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
BASE COUNT         1097 a         1191 c         1136 g         1401 t
ORIGIN      
        1 gggtattttg cgctacgcgc tcatcagtga ctttcaactg acccacgtta accgcatcgg
       61 tatccgctga accagctgcg acgtttgtga tctgacgttg accatccgca ctaccgactg
      121 agaattcacc gacagagttc tgcggtgcgt ccatgttgta ggcggtataa ttggtttgag
      181 cgccacgagt ggtcgtagaa ccattaccta tcgcaatact gttgacatga atgacttgcg
      241 cattgctacc aatggcaatg ccgttaagtg cattcgcgtc cacgtaggca ccataaccga
      301 taccaatacc tttatcgcca ttcacgatgg ttttcgcacc cattgccagc gagtcagttc
      361 ccactgccag tgaatcttct tccgttgaat tagcgtggaa gtatttagtc ggcgtggttg
      421 cgaccgcacc aatcgcattc tgcaattgac gaaccgtaac ggcgtcatgg gcttcggaac
      481 catcggctac gttgatgatt tgacgatatt taccgtcatc accgatagac aatgcaccga
      541 gcagttcgcc atccgtggtg tcgtaaccaa taacaacgcc attttccgtt atgctggtgt
      601 cacgggagcc tttggcaatc actcggctgg aaacagagct gctacctagg gcgatacccg
      661 tatcaacgtc gctgtagctg ccctgaccaa tagctacgct gctatcgcct ttggcgacag
      721 agttataacc tatagctgta gcgccaacac cctgtgcgct ggcgtcgttg aacgctaagc
      781 cgtcgtcgtt agtacgcaca tagttaatac ccgcaccgtt ttcttggata taggttgcgt
      841 cggtgttacc agcgagctga ttgataattt gggtgttctg ctcaatcatc atattcgtcg
      901 cattcaactg agaaccgttc actgcatcgg tactgtcctc actaatactg ccattagcga
      961 cattagtgat gatgctggct ttaccgtcat ggctggcatt gtaggctcca gcaccgccat
     1021 tggcagtctc atcccacagc agggcgttat catcaagcgc atccagagcg tcaccgacgt
     1081 tgttgtactc ggcgttcgca atggtgtaag tcggtgcggt gatggtgccg tcagcgttga
     1141 cttctgcgtc accatccagc gcatcagcga tgtacttatt ggtggtatag agttgtgagc
     1201 cgttaatcgc gtctctgctg gcagcagaga ttgcaccgtt agcgacgtta gtgattacac
     1261 tggcagtttt atcttttccg tgagcggcgc taaatgcacc attttcaccg gcgtccgcat
     1321 cccagagcag agcgtcgtca agagtggtat cgatagcatt cagggcatca ccgacattat
     1381 cgtaatcagc attagcaatg gtgtacgtcg gcgcagtgat ggtgccgtct gcattgactt
     1441 cggcaccacc ccccagcgca tcaacaacat aactgctcac gccgtggagt tgtgagccgt
     1501 ttactgcgtc agaactggag tctgaaattt caccatctgc gacatcagtg atcacgcttg
     1561 cgtcaccatt agtaccgtgt ttggcactga atttacctgc ggtggcatcc caaagcagag
     1621 catcgccgag agacgtgcta aatgaggagt taatcgcagc cagtgcatcg ccgacgttat
     1681 aataatttgt ttcaccgatt ttgtaggttg gaccggtaaa cacaccgtca gtattcacag
     1741 aagcattgcc gccgaagtag gtggcgatat tgctgcttaa gtcataaagc tggctaccgt
     1801 taatggcatc ggaactggtt gcagtgactg tgccgtccag gatattggtg attttgctgg
     1861 cggtattgtt gccatgagct gcagtgaaga caccattgtc cttatcccat ttcagcgcat
     1921 cctcaccaag attagtcacc gtctcagtca gattagagat gttggtggtg ttagtggcga
     1981 tattggtggt attcgtcgcc acggcatcgt tggtggtttt cagctgagag ccgttaacgg
     2041 catcggtgct gccagtcgtc aggtcgccat ctttaacgtt ggtgattttg ctggtggtct
     2101 cggtgccatg agctgccgtg aatacaccat tgtccttatc ccatttcagc gcatcctcac
     2161 caagattagt caccgtctca gtcagattag agatgttggt ggtgttagtg gcaatattgg
     2221 aagtgttatt ggcgatattg gtggtattcg tcgccacagc atcgttggtg gttttcagct
     2281 gagagccgtt aacagcatca gtgctgtcag ccgtcaggtc ggcatcttta acgttggtga
     2341 ttttgctggt ggtatcctgg ccgtgagctg cactgaatgc ctttttagtt tcgttccaga
     2401 gcagggcatc agcctgaagg tcaccaacgg aatccgtcag gttggtgata ttggtggtat
     2461 tggtggcaat atttgccgta ttcgtggcaa tgttgctggt attagtagcg atattggcgg
     2521 tgttggcttc gacatcgtca ttggtagctt tcagttggga accgttaacc gcatccttac
     2581 tggaggctga aatcgtgcca tccgcaacat cggtgattac gctggcagtt gggctactag
     2641 taccatgagc agcgctgtat ttgcctttgg tttggtccca ttgcagggtg ttttcatcaa
     2701 gtacagcgag cgcagcccct acgttatttt tgctaccatt ttttaaattg taggttggtg
     2761 ctgtaacagt accgtcatca acatctactg cagcccctcc tccaagcctt tttgctaccg
     2821 agtcgctaat ggcataaagc tgtgaaccat taatggcatc gtaactgtca gacttgatcg
     2881 cgccattttt cacattaacg atcttgcgtt ttaatgaact attgccgaca gagacggtat
     2941 tgtctaaatc cgcaatggaa tctgcgccca gcgcgacaga gttaataccg ttggcactgc
     3001 tattactacc aagcgcaaga ctcttatcac cagttgcatt actggctgtg cctaaggcaa
     3061 tggtgtttaa gcctctcgac tggctaccat tacccagagc gatggcatcc acgccatcag
     3121 ctttgttgcc ctggcctatg gcgattgcat tatctgccag agcctggctg ccatttccta
     3181 atgcgatagt attgacttta tcggcaaggc ttccgaaccc cattgccatg ctattagtcc
     3241 cttttgcctt cgcattattg ccaatagcag ttgcattttc agcggcttcg gtctcagcac
     3301 ctatcgcaat agcgttcgct gcgctggcaa gtgattgtct gccaaatgcg aggctgtcag
     3361 taccgctagc tacactgcta cgccccaggg caattgcgtt ttcttcagat gcgacgctac
     3421 ttgctcccag cgccattgaa tacgcttttg acgcattggc ggtgtcgcca agagcaatac
     3481 tcataatctc agtagcttta gtggcatttc ccagggcaat ggcgtttgct ccttccgctt
     3541 tggaagagtc ccccatagca atagacaatt tacccaatgc ttttgaataa cggccgaggg
     3601 ccattgagta ttggcccaat gaatacgaag aggcacccag tgctatactt ctatcgcctt
     3661 ctgatattgc actaacccca aaggccattg atgcaccacc aatcgcatgg gtttttgacc
     3721 cgatggcaga gctatattgg ccttcagcta tagcgtcata acccacagca gtactggaac
     3781 cactggtgtt cataaaagta tttgctttcg cccctttgcc tatagcaacc cagccgtcgc
     3841 cagctgatcc actaccgtaa tcaacaccct gaccgttgtc attcccggca ttcgccaatg
     3901 ccccaaacga cgacaacatt ccacccgcaa ccagcgcaga aattaacagc ttactgcgcc
     3961 cagatttctt gccacggctt tttgccgttt cgctggtaac agtataattc cctgtcgcag
     4021 ggttccagat aactttaaat attttgttca ttaatgatgc tcgctattcc ttgttataag
     4081 tgtactaact gcatttattt tttgcttctt ttctgccgat tttcagcaga gggaagattg
     4141 tcgaactgaa tattttccat gttgatggct aacttcagat atcccaggtt attgggcgag
     4201 acacccttca gggtcaaatt aatgtcttgc cattgacctg gtaataattt caatgtggat
     4261 ttcattgatg tgttgaaaag taattgttgc caccgggcaa actctttggc atcgacaggt
     4321 ttttcaccac cccatgtcgc attaaaacca atatttgaga taggtgtact taataagttg
     4381 accagtgaaa tatgaataac actgccattc tctgtggcgt caatggataa cactcgaccc
     4441 gctaaagcca ccgaaccatt gttcaggatg aaatacttcg tttttgctgg gttaaactcg
     4501 gtaaaaataa aagtgttttg ttgatcttta acggatttaa ttacgtgagt taaagtcttt
     4561 gtactgctta ttatgccagt ttgttgtttt tgaatagttt ccagttgact ctttagctgt
     4621 gcttgttgtt tttcaagtag atcaatttta ttgttatgtt cttcttgttg tttcaataaa
     4681 ctactttctg gcttgccttc ttcaatgtag tcacatcccg ttaatgcgca gagaactgaa
     4741 acagtgacag cactgaaaaa aatcttcttc aagttcatgt aaaaatcctt atttcctccg
     4801 accagcgatc aatgccggta gagtt
//
LOCUS       DAJVUJ010000222         4795 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.224, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000222 DAJVUJ010000000
VERSION     DAJVUJ010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4795
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.224"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..802
                     /locus_tag="OQQ48_003938"
     CDS             <1..802
                     /locus_tag="OQQ48_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YadA family autotransporter adhesin"
                     /protein_id="HCS1954519.1"
                     /translation="QSITNLNNQVTNLDTRVTNIENGIGDIVTTGSTKYFKTNTDGVD
                     ANAQGKDSVAIGSGSIAAADNSVALGTGSVAEEENTISVGSSTNQRRITNVAAGVNAT
                     DAVNVSQLKSSEAGGVRYDTKADGSIDYSNITLGGGNGSTTRISNVSAGVNNNDAVNY
                     AQLKQSVQETKQYTDQRMVEMDNKLSKTESKLSGGIASAMAMTGLPQAYTPGASMASI
                     GGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAALGAGIQW"
     gene            1170..2825
                     /gene="lldP"
                     /locus_tag="OQQ48_003939"
     CDS             1170..2825
                     /gene="lldP"
                     /locus_tag="OQQ48_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HCS1954520.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            2825..3601
                     /gene="lldR"
                     /locus_tag="OQQ48_003940"
     CDS             2825..3601
                     /gene="lldR"
                     /locus_tag="OQQ48_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HCS1954521.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            3598..4788
                     /gene="lldD"
                     /locus_tag="OQQ48_003941"
     CDS             3598..4788
                     /gene="lldD"
                     /locus_tag="OQQ48_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HCS1954522.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADTHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPAALAPMAK
                     GNAA"
BASE COUNT         1055 a         1274 c         1300 g         1166 t
ORIGIN      
        1 ccagagcatt actaacctga acaatcaggt cactaatctg gatactcgcg ttactaatat
       61 cgaaaacggt attggcgaca ttgtcaccac cggtagcacc aagtacttca agaccaacac
      121 cgatggcgta gatgccaacg cccagggtaa agatagcgtt gctattggtt ctggttccat
      181 tgctgccgct gacaacagcg tcgcactggg taccggttcc gttgcagagg aagaaaatac
      241 aatctctgta ggttcttcta ctaaccaacg ccgtatcacc aacgtagctg ccggtgttaa
      301 tgccaccgat gcggttaacg tgtcgcagct gaaatcttct gaagcaggcg gcgtgcgcta
      361 cgacaccaaa gctgatggtt ctatcgacta tagcaatatc accctcggtg gtggcaacgg
      421 cagtacgact cgtatcagca acgtctccgc tggcgtcaac aacaacgacg cggtgaacta
      481 cgcgcagttg aagcaaagcg tgcaggaaac gaagcaatac accgatcagc ggatggttga
      541 gatggataac aaactgtcta aaactgaaag caagttgagc ggtggtatcg cttctgcaat
      601 ggcaatgacc ggtctgccgc aggcttatac accgggtgcc agcatggctt ctattggtgg
      661 cggtacttac aacggtgaat cggcagttgc tttaggtgta tcgatggtga gcgccaatgg
      721 tcgttgggtc tacaaattac aaggtagtac caatagccag ggtgaatact ccgccgcact
      781 cggtgccggt attcagtggt aatgatccat taagttagtg tgactaaggg caaacgccaa
      841 attgcctgat gcgttacgcg catcaggcaa gataaggcgc tccgggtcaa caatctaagg
      901 gcaattctct gatgaggatt gcccttttct ttaccagaca tctcccccca caagaattgg
      961 ccctaccaat tcttcgctta tctgacctct ggttcacaat tccccaatta aaactcacat
     1021 caatattgcc aatacacaac atttagttaa ccattcattg tcattatccc tacacaacac
     1081 aattggcagt gccactttta cacaacgtgt gacaaggaga tgagcaacag actcattaca
     1141 cgatgtgcgt ggactccagg agacctgcaa tgaatctctg gcaacaaaac tacgatcccg
     1201 ccgggaatat ctggctttcc agtctgatag catcgcttcc catcctgttt ttcttctttg
     1261 cgctgattaa gctcaaactg aaaggatacg tcgccgcctc atggacggta gcaatcgccc
     1321 ttgccgtggc tttgctgttc tataaaatgc cggtcgctaa cgcgctggcc tcggtggttt
     1381 atggcttctt ctatggtttg tggcccatcg cgtggatcat tattgcagca gtgttcgtct
     1441 ataagatctc ggtgaaaacc gggcagtttg acatcattcg ctcgtctatt ctttcgataa
     1501 cccctgacca gcgtctgcaa atgctgatcg tcggtttctg tttcggcgct ttccttgaag
     1561 gagccgcagg ctttggcgca ccggtagcaa ttaccgccgc attgctggtc ggcctgggtt
     1621 ttaaaccgct gtacgccgcc ggactgtgcc tgattgttaa caccgcgcca gtggcatttg
     1681 gcgcgatggg cattccaatc ctggttgccg gacaagtaac aggcatcgac agttttgaga
     1741 ttggccagat ggtggggcgt cagctaccgt ttatgaccat tatcgtgctg ttctggatca
     1801 tggcgattat ggacggctgg cgcggtatca aagagacgtg gcctgcggtc gtggttgcgg
     1861 gcggctcgtt tgccatcgct cagtacctta gctccaactt cattgggccg gagctgccgg
     1921 acatcatctc ttcgctggta tcactgctct gcctgacgct gttcctcaaa cgctggcaac
     1981 cagttcgcgt attccgcttc ggtgacttgg gggcgtcaca ggttgatatg acgctggccc
     2041 acaccggtta caccgcgggt caggtgttac gtgcctggac accgttcctg ttcctgaccg
     2101 ctaccgtaac actgtggagc atcccgccgt ttaaagccct gttcgcatcg ggtggtgcgc
     2161 tgtatgagtg ggtgatcaac attccggtgc cgtacctcga taaactggtt gcccgtatgc
     2221 caccagtggt cagcgaggct acagcctatg ccgccgtgtt taagtttgac tggttctctg
     2281 ccaccggcac cgccattctg tttgctgcac tgctctcgat tgtctggctg aagatgaaac
     2341 cgtctgacgc tatcagcacc ttcggcagca cgctgaaaga actggctcta cccatctact
     2401 ccatcggcat ggtgctggcg ttcgccttta tctcgaacta ttccggactg tcatcaacac
     2461 tggcactggc actggcgcac accggtcatg cattcacctt cttctcgccg ttcctcggct
     2521 ggctgggggt attcctgacc gggtcggata cctcatctaa cgccctgttc gccgcgctgc
     2581 aagccaccgc agcacaacaa attggcgtct ctgatctgtt gctggttgcc gccaatacca
     2641 ccggtggcgt caccggtaag atgatctccc cgcaatctat cgctatcgcc tgtgcggcgg
     2701 taggcctggt gggcaaagag tctgatttgt tccgctttac tgtcaaacac agcctgatct
     2761 tcacctgtat ggtgggcgtg atcaccacgc ttcaggctta tgtcttaacg tggatgattc
     2821 cttaatgatt gttttaccca gacgcctgtc agacgaggtt gccgatcgtg tgcgggcgct
     2881 gattgatgaa aaaaacctgg aagcgggcat gaagttgccc gctgagcgcc aactggcgat
     2941 gcaactcggc gtatcacgta attcactacg cgaggcgctg gcaaaactag tgagcgaagg
     3001 cgtgctgctc agtcgacgcg gcggcgggac gtttattcgc tggcgtcatg acacatggtc
     3061 ggagcaaaac atcgtccagc cgctaaaaac gctgatggcc gatgatccgg attacagttt
     3121 cgatattctg gaagcccgct acgccattga agccagcacc gcatggcatg cggcaatgcg
     3181 cgccacacct ggcgacaaag aaaagattca gctttgcttt gaagcaacgc taagtgaaga
     3241 cccggatatc gcctcacaag cggacgttcg ttttcatctg gcgattgccg aagcctcaca
     3301 taacatcgtg ctgctgcaaa ccatgcgcgg tttcttcgat gtcctgcaat cctcagtgaa
     3361 gcatagccgt cagcggatgt atctggtgcc accggttttt tcacaactga ccgaacaaca
     3421 tcaggctgtc attgacgcca tttttgctgg tgatgccgac ggggcgcgta aagcaatgat
     3481 ggcgcacctt agttttgttc acaccaccat gaaacgattc gatgaagatc aggctcgcca
     3541 cgcacggatt acccgcctgc ccggtgagca taatgagcat tcgagggaga aaaacgcatg
     3601 attatttccg cagccagcga ttatcgcgcc gcagcgcaac gcattctgcc gccgttcctg
     3661 ttccactata tggatggtgg tgcatattct gaatacacgc tgcgccgcaa cgtggaagat
     3721 ttgtcagaag tggcactgcg ccagcgtatt ctgaaaaaca tgtccgactt aagcctggaa
     3781 acgacgctgt ttaatgagaa attgtcgatg ccggtagcac tggcaccggt gggtttgtgt
     3841 ggcatgtatg cgcgtcgtgg cgaagttcag gcagccaaag cggcggacac gcatggcatt
     3901 ccgtttactc tctcgacggt ttccgtttgc ccgattgaag aagtcgcgcc agccatcaag
     3961 cgcccaatgt ggttccagct ttatgtactg cgcgatcgcg gctttatgcg taacgcgctg
     4021 gagcgagcaa aagcagcggg ttgttcgacg ctggttttca ccgtggatat gccgacaccg
     4081 ggcgcacgct accgtgatgc gcattcaggt atgagcggcc caaatgcggc aatgcgccgc
     4141 tacttgcagg cggtgacgca tccgcaatgg gcgtgggatg tgggcctgaa cggtcgtcca
     4201 catgatttag gtaatatctc agcttatctc ggcaaaccaa ccggactgga agattacatc
     4261 ggctggctgg ggaataactt cgatccgtcc atctcatgga aagaccttga gtggatccgc
     4321 gatttctggg atggcccgat ggtgatcaaa gggatcctcg atccggaaga tgcgcgcgat
     4381 gcagtacgtt ttggtgctga tggaattgtg gtttctaacc acggtggccg ccagctggac
     4441 ggtgtactct cttccgctcg tgcgttgccc gctattgcag atgcggtgaa aggtgatatc
     4501 gccattctgg cggatagcgg aattcgtaac gggcttgatg tcgtgcgtat gattgcgctc
     4561 ggtgccgaca ccgtactgct gggtcgtgct ttcctgtatg cgctggcaac agcgggccag
     4621 gcgggtgtag ctaacctgct aaatctgatc gaaaaagaga tgaaagtggc gatgacgctg
     4681 actggcgcga aatcgatcag tgaaattacg caagattcgc tggtgcaagg gctgggtaaa
     4741 gagttgcctg cggcactggc tcccatggcg aaagggaatg cggcatagtc gtttg
//
LOCUS       DAJVUJ010000223         4737 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.225, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000223 DAJVUJ010000000
VERSION     DAJVUJ010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4737
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.225"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(<1..233)
                     /locus_tag="OQQ48_003942"
     CDS             complement(<1..233)
                     /locus_tag="OQQ48_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1954523.1"
                     /translation="MSRKNQRYSKEFKAEAVRTVLENQLSISEGASRLSLPEGTLGQW
                     VTAARKGLGTPGSRTVAELESEILQLRKALNEAR"
     gene            297..1388
                     /gene="nadB"
                     /locus_tag="OQQ48_003943"
                     /pseudo
     CDS             297..1388
                     /gene="nadB"
                     /locus_tag="OQQ48_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
     gene            complement(1373..2110)
                     /gene="trmN"
                     /locus_tag="OQQ48_003944"
     CDS             complement(1373..2110)
                     /gene="trmN"
                     /locus_tag="OQQ48_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HCS1954524.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWLTQQTVRFDLIISNPPYYQQGVECAIPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            2242..3576
                     /gene="srmB"
                     /locus_tag="OQQ48_003945"
     CDS             2242..3576
                     /gene="srmB"
                     /locus_tag="OQQ48_003945"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HCS1954525.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIEELRPKTRTPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            complement(3785..4666)
                     /locus_tag="OQQ48_003946"
     CDS             complement(3785..4666)
                     /locus_tag="OQQ48_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1954526.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKGSDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     LSIESIKRCVAANIGVSYLPRFAVEKELESGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCIEECFLPG"
BASE COUNT         1193 a         1282 c         1197 g         1065 t
ORIGIN      
        1 cgagcttcat ttaacgcctt acgcagttgc agaatttcag attccagttc agccaccgtg
       61 cgggaaccag gagtaccgag cccttttctg gcggcggtaa cccattgtcc taaagtgcct
      121 tcaggaaggg ataatcggga agcgccttca ctgatcgaaa gttgattttc aagaaccgtt
      181 ctgacagctt cggctttgaa ctctttagag taacgttggt tttttctgct cattattagc
      241 tccttctgat gccattctat ttcaggaagg agtgtccgtt aaactcaggc tacctcaggg
      301 ttgtaggcgc gtgggtatgg aaccgaaata aagaaacggt ggaaacctgc cacgcaaaag
      361 cagtggtact ggcaaccggc ggtgcgtcga aggtgtatca gtacaccacc aatccggata
      421 tttcttctgg cgatggcatt gctatggcgt ggcgcgcggg ctgtcgggtt gccaatctcg
      481 aatttaatca gttccaccct accgcactgt atcacccaca ggcacgcaat ttcctgttaa
      541 cggaagcact gcgcggcgaa ggcgcttatc tcaagcgccc ggatggcacg cgttttatgc
      601 ccgattttga tgagcgcggc gaactggccc cgcgcgatat tgtcgcccgc gccattgacc
      661 atgaaatgaa acgcctcggc gcagattgta tgttcctcga catcagccat aagcccgccg
      721 attttattcg ccagcatttc ccgatgattt atgaaaaatt gctcgggctg gggattgatc
      781 tcacacaaga accggtaccg attgtgcctg ctgcacacta tacctgcggt ggtgtaatgg
      841 ttgatgatca tgggcgtacg gacgtcgagg gcttgtatgc cattggcgag gtgagttata
      901 ccggcttaca cggcgctaac cgcatggcct cgaattcatt gctggagtgt ctggtctatg
      961 gctggtcggc ggcagaagat atcaccagac gtatgcctga tgcccacggc gtcagtacgt
     1021 taccaccgtg ggatgaaagc cgcgttgaga accctgacga acgggtagta attcagcata
     1081 actggcacga gctacggctg tttatgtggg attacgtcgg cattgtgcgc acaacaaaac
     1141 gtcttgagcg cgccctgcgg cgtatcaata tgttgcaaca ggaaatagac gaatattacg
     1201 cccacttccg cgtgtcgaat aatttgctgg agctgcgtaa tctggtgcag gttgcggaat
     1261 tgattgttcg ctgtgccatg atgcgcaagg agagtcgtgg cctgcatttc acactggatt
     1321 atccggaact gctcacccat tccggtccgt cgatcctttc ccccggcaat cattacataa
     1381 acagataaaa agcctgggtc agcgccgtat acgcttcgga atagttctgg tctggcccac
     1441 gaatgactaa gcgatcgcta aagcattctc ccgcctgcgg ggagaatgcc agcagcaccc
     1501 gatgcggcag tcgcgcttcg ttttccgcca catccgtccg caaacgtaaa tgccagccca
     1561 tgcttaatgc cagctccgta aaaccattac caatctgctc tggcagcact acgcagaaaa
     1621 atccctcttc ggtaatgcac tccgccgcac aggtcagcag cgatgggtga tcaagcgtag
     1681 tggtatagcg agcctgttcc cgttgaggga tcgcgcactc tactccctgc tgatagtaag
     1741 gtgggttact gatgattaaa tcgaagcgta ctgtctgctg tgtgagccac tgctgaatat
     1801 ccgccgtatg gacgttaatc cgctctgccc acggggactg gttgatattt tcctgcgcct
     1861 gcgcggcagc ttcactttcc agctcaacgg catcaattat cacgctgtca ctggttcgct
     1921 gcgccagcat cagtgccagc aacccgctac ccgcgccgat atcaaggcaa cgtttcaccc
     1981 cagccaccgg tgcccatgcg cccagcaaaa tgccatccgt tcccactttc atcgcacagc
     2041 gatcgtgagc aacaaaaaac tgtttaaaag taaatccatt acgacgaagc acggatgtag
     2101 actgtgacat gaaaataaaa ccttgcagga aaaacggcga tagcaccggg tgagaacaat
     2161 acccgagaag cgatatccat acaaacagat gaagattgca gccgtaacgt ctataatcag
     2221 cgccccacac agaggtagaa catgactgta acgacttttt ccgaacttga actcgacgaa
     2281 agcctgctgg aagccctcca ggataaaggt ttcactcgcc cgaccgccat tcaggctgcc
     2341 gccattccgc ctgcgctcga tggccgtgat gtactcggtt ctgcgccgac aggcaccggt
     2401 aaaacggcgg cgtatctgct gccagcgttg cagcacctgc tcgatttccc gcgtaagaaa
     2461 tccggtccgc cgcgtatttt gatcctcacc ccaactcgcg agctggcgat gcaggtggcc
     2521 gatcatgccc gcgaactggc gaaacatacg catctggata tcgccaccat taccggcggc
     2581 gtagcctata tgaaccacgc ggaagtgttc agcgaaaatc aggacatcgt ggtcgccacg
     2641 accggacgtc tgctgcaata cataaaagaa gagaacttcg attgccgcgc ggttgaaacg
     2701 ctgatcctcg acgaagcaga ccgtatgctg gatatgggct tcgctcagga tatcgaacat
     2761 attgctggcg aaacgcgctg gcgtaaacag accctgctct tttcggcaac gctggaaggc
     2821 gatgcgattc aggactttgc cgagcgtctg ctggaagatc cggtggaggt ttctgccaat
     2881 ccgtccaccc gtgagcgcaa aaaaattcat cagtggtatt accgcgccga tgacctggaa
     2941 cataaaaccg cattgctggt gcatctgtta aaacagccgg aagcgacccg ctcaattgtg
     3001 tttgtgcgta agcgtgaacg tgtgcatgag ctggcaaact ggctgcgcga agcgggcatc
     3061 aacaactgct atctcgaagg tgagatggta cagggcaagc gtaatgaagc gattaaacgc
     3121 ctgaccgaag gccgcgtaaa cgtactggta gcaaccgatg ttgccgcgcg cggtatcgac
     3181 attccagacg tcagccacgt ctttaacttc gatatgccgc gcagtggtga tacttatttg
     3241 caccgtatcg gacgtaccgc gcgcgccggt cgtaaaggca ccgcgatttc gctggtggaa
     3301 gcccatgacc atctgctgct tggcaaagtt ggccgttata ttgaagagcc gataaaagct
     3361 cgcgtcattg aggaactacg cccgaaaacg cgtacgccaa gcgagaagca gaccggcaag
     3421 ccatcgaaga aagtactggc aaaacgcgcc gagaagaaaa aagctaaaga gaaagagaag
     3481 ccgcgggtga aaaaacgcca tcgcgacacc aaaaatattg gtaagcgccg taaaccgagc
     3541 ggaacgggcg tgccaccgca aacgacagaa gagtaatctt aattccgggt ttaagcccgg
     3601 aattaaaagt gctggcaaac gcaaaactgc ctgatgcgct acgcttatca ggcctacatg
     3661 gctcatgcaa tatattgaat ttgcacgatc ttgtaggccg gataaggcgt tcacgccgca
     3721 tccggcatga acaaaacgca cttcgtcgac aagttgggtg ccgggtttaa gcccggcatt
     3781 atttttatcc cggcaaaaaa cactcttcaa tacactgaat aaaagtgtgc atcgccgggc
     3841 taaccgcttt tccagcatgg tgtgcgcaca tagccgtaat ggtctgcgac tgttcgccaa
     3901 aaggcaattc aattaattct ccgctctcaa gctctttttc caccgcaaag cggggcaagt
     3961 aactgacacc gatattcgcc gcgacacaac gtttgatact ttcaatgctt aacagctcaa
     4021 tggtgttttc caccgtgatc cgccgctgac gcagcgtgct ctcaaatatc tgccggaaga
     4081 cacattgcgg ttcgttgata ataaagctac aggcgttatg tcttcccggc tcagtaaaat
     4141 cgacatctgc aatttgcggt gaagccacca gcaccagcga ttgttcaccc aactctcgtc
     4201 ggttcagggc atcatcattc cctacgcgat aaaagacgcc gacatcggcc tcatcattca
     4261 gtagcgcatc acggatcacg taacagttca gcgactgcaa cgataaacgc acttttggtg
     4321 cgcgttgccg aaaacgctgc aacacctgcg gcattcggta agagagcaac gtttcgcccg
     4381 aaacaacgcg tagttccccg tccggatctg accccttttt ggcagcctca cgaagcgtat
     4441 ccatcactcg ggtaagttca taaatgtgcg gcagcaactt ttttccttcg cgggtaaggc
     4501 acatccgtcg gccaattttc tcaaataact ggactgaaaa ctcctgctca agctgctgaa
     4561 tatgaaaagt caccgtcgat tgtgtacagc acaatttttg cgaagctcgc aaaaaggaac
     4621 cctcttccac cacggtttta agcgtaataa aacggcgcag atccataacc ccgaacctat
     4681 cgaaaatatc gaatctagaa tataaaaaca ttcatttttt taaatgttct gtgtcgg
//
LOCUS       DAJVUJ010000224         4710 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.226, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000224 DAJVUJ010000000
VERSION     DAJVUJ010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4710
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.226"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            80..1042
                     /gene="tauA"
                     /locus_tag="OQQ48_003947"
     CDS             80..1042
                     /gene="tauA"
                     /locus_tag="OQQ48_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCS1954527.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQAGNT
                     FAKESGASVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKLGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKVIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            1055..1822
                     /gene="tauB"
                     /locus_tag="OQQ48_003948"
     CDS             1055..1822
                     /gene="tauB"
                     /locus_tag="OQQ48_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HCS1954528.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQVLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            1819..2628
                     /gene="tauC"
                     /locus_tag="OQQ48_003949"
     CDS             1819..2628
                     /gene="tauC"
                     /locus_tag="OQQ48_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HCS1954529.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVSPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTP"
     gene            2643..3494
                     /gene="tauD"
                     /locus_tag="OQQ48_003950"
     CDS             2643..3494
                     /gene="tauD"
                     /locus_tag="OQQ48_003950"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HCS1954530.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGITAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNCVTQHYANADYLPQRRIMHRVTILGD
                     KPFYRAG"
     gene            complement(3600..4574)
                     /gene="hemB"
                     /locus_tag="OQQ48_003951"
     CDS             complement(3600..4574)
                     /gene="hemB"
                     /locus_tag="OQQ48_003951"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HCS1954531.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
BASE COUNT         1048 a         1216 c         1383 g         1063 t
ORIGIN      
        1 gcgataacga ctaattcttt taatgaatgt ttttattcct gaatactgct cccataacaa
       61 gactggggag cagacaatca tggcaatttc atcgcgtaac acacttcttg ccgcactggc
      121 attcatcgct tttcaggcac aggcggtgaa cgtcaccgtg gcgtatcaaa cctcagccga
      181 accggcgaaa gtggctcagg ccggcaacac ctttgctaaa gagagcgggg cgagcgtgga
      241 ctggcgtaag tttgacagcg gagccagcat cgtgcgggcg ctggcttctg gcgacgtgca
      301 aatcggcaac ctcggttcca gcccattagc ggttgcagcc agccaacagg tgccgattga
      361 agtcttcttg ctggcgtcaa aactgggtaa ctccgaagcg ctggtggtaa agaaaactat
      421 cagcaaaccc gaagatttga ttggcaaacg cattgccgta ccgtttatct ccaccaccca
      481 ctacagcctg ctggcggcgc tgaaacactg ggggattaaa ctagggcaag tggagattgt
      541 gaacctgcag ccgcccgcga ttatcgccgc atggcagcgg ggtgatattg atggtgctta
      601 tgtctgggca ccggcggtta acgccctgga aaaagacggc aaggtgttga ccgattctga
      661 acaggtcggg cagtggggtg cgccaacgct ggacgtctgg gtggtgcgca aagattttgc
      721 cgagaaacat cctgaggtcg tgaaagcgtt cgctaaaagc gccatcgatg ctcagcaacc
      781 gtacattgct aacccagacg cgtggctgaa acagccggaa aacatcagca aactggcgcg
      841 tttaagcggc gtgccggaag gtgacgttcc ggggctggtt aaggggaata cctatctgac
      901 gccgcagcaa caaacggcag aactgaccgg accggtgaac aaagtaatca tcgacaccgc
      961 gcagtttttg aaagagcagg gcaaggtccc ggctgtagcg aatgattaca gccagtacgt
     1021 tacctcgcgc ttcgtgcaat aaaaggaggc gcagatgctg caaatctctc atctctatgc
     1081 cgattatggc ggcaaaccgg cactggaaga tatcaacctg acgctggaaa gcggcgagct
     1141 actggtggtg ctggggccgt ccggctgcgg taaaaccacg ttgctgaatc tgattgccgg
     1201 ttttgtgcct tatcagcatg gcagcattca actggcgggt aagcgtattg agggaccggg
     1261 agcagagcgc ggggtggttt ttcagaatga agggctgcta ccgtggcgca atgtacagga
     1321 caacgtggcg ttcggcctgc aattggcagg tatagagaaa atgcagcgac tggaaatcgc
     1381 gcaccaggtg ctgaaaaaag tggggctgga aggcgcagaa aaacgctata tctggcagct
     1441 ttccggtggt caacgtcagc gggtggggat tgcccgcgcg ctggcggcga atccccagct
     1501 gttattactc gacgaaccgt ttggtgcgct ggacgccttc acccgcgacc agatgcaaac
     1561 tctgctgctg aaactctggc aggagacggg caagcaggtg ctgttgatta cccacgatat
     1621 agaagaagcg gtgtttatgg cgactgaact ggttctgctt tcatccggcc ctggccgtgt
     1681 gctggagcgg ctgccgctca actttgctcg ccgctttgtt gcgggagagt cgagccgcag
     1741 catcaagtcc gatccacaat tcatcgccat gcgcgaatat gttttaagcc gcgtatttga
     1801 gcaacgggag gcgttctcat gagtgtgctc attaatgaaa aactgcattc gcggcggctg
     1861 aaatggcgct ggccgctctc gcgtcaggtg accttaagca ttggcacgtt agcggtttta
     1921 ctcactgtat ggtggacggt ggcggcgctg caactgatta gcccgctgtt tttgccgccg
     1981 ccgcaacaag tactggcaaa actgctcacc attgccggac cgcagggatt tatggacgcc
     2041 acgctgtggc agcatctggc agccagcctg acgcgcattg tgctggcgct actcgcggcg
     2101 gtggtgatcg gtattccggt cgggatcgcc atggggctaa gcccgacggt gcgcggcatt
     2161 ctggatccgg taatcgaact ttatcgtccg gtgtcgccgc tggcttattt gccgctgatg
     2221 gtgatctggt ttggtattgg tgaaacctcg aagatcttac tgatctattt agcgattttt
     2281 gcaccggtgg cgatgtcggc gctggcgggg gtgaaaagcg cgcagcaggt tcgcattcgt
     2341 gccgcccagt cgctgggtgc cagccgtgcg caggtgctgt ggtttgtcat tttgcccggc
     2401 gcgctgccgg aaatcctcac cggattacgc attggtctgg gtgtgggctg gtctacgctg
     2461 gtagcggcgg agctgattgc cgcgacgcgc ggtttaggat ttatggttca gtcagcgggt
     2521 gaatttctcg cgactgacgt ggtgctggcg ggaatcgcgg tgattgcgat tatcgccttt
     2581 cttttagaac tgggtttgcg cgcgttacag cgccgtctga cgccctagca tggagaagta
     2641 caatgagtga acgtctgagc attaccccgc tggggccgta tatcggcgca caaatttcgg
     2701 gtgccgacct gacgcgcccg ttaagcgata atcagtttga acagctttac catgcggtgc
     2761 tgcgccatca ggtggtgttt ctgcgcgatc aagccattac gccgcagcag caacgcgcgc
     2821 tggcccagcg ttttggcgaa ctgcatattc accctgttta ccctcatgcc gaaggggttg
     2881 acgagatcat cgtgctggat acccataacg ataatccgcc agataacgac aactggcata
     2941 ccgatgtgac atttattgaa acgccacccg caggggcgat tctggcagct aaagaattac
     3001 cttcgaccgg cggtgatacg ctctggacca gcggtattac ggcctatgag gcgctctctg
     3061 ttcccttccg ccagctgctg agcgggctgc gtgcggagca tgatttccgt aaatcgttcc
     3121 cggaatacaa ataccgcaaa accgaggagg aacatcaacg ctggcgcgag gcggtcgcga
     3181 aaaatcctcc gttgctacat ccggtggtgc gaacgcatcc ggtgagcggt aaacaggcgc
     3241 tgtttgtgaa tgaaggcttt actacgcgaa ttgtcgatgt cagcgagaaa gagagcgaag
     3301 ccttgttagg ctttttattc gcccatatca ctaaaccgga gtttcaggtg cgttggcgct
     3361 ggcaaccgaa tgatattgcg atttgggata actgcgtgac tcagcactat gccaatgccg
     3421 attacctgcc acagcggcgg attatgcatc gggtgacgat tcttggggat aaaccgtttt
     3481 atcgggcggg gtgatacgag tgcggacggt tccctcgccc ccttggggag agggttaggg
     3541 tgagggggcg ttcaccgtac tttcaacagg ttaactcccc ctttctgaga ggaaaaaaat
     3601 taacgcagaa tcttcttctc agccaaatcc agcgcaaagt agctgaaaat cagatccgca
     3661 cccgcacgct taattgaacc taagctttcg agcacgactt tctcttcatc tatagcaccc
     3721 gccagcgcgg cgaacttaat catcgcgtac tcaccgctca cctgatacgc gccaatcggc
     3781 aattcagtac gttcacgcag ctcacgcacg atgtcgaggt acgctccagc aggtttaacc
     3841 atcaggcagt ctgcgccctg ggcttcatcc agcagtgact cacgaatcgc ctcgcgacgg
     3901 ttcatagggt tcatctgata gcttttgcgg tcgcctttta atgcgcttcc ggcagcttca
     3961 cggaacgggc cataaaagga agaggcgaac ttggtcgaat acgacataat cgccgtatct
     4021 ttaaagcctg cagcgtccag cgcctgacga atcgcctgta cctggccgtc catcgcggcg
     4081 gaaggggcga tgaagtctgc gcctgcagca gctgcaacca cggcttgctt gcctaaattt
     4141 tccagagtcg cgtcgttgtc gacgccatgc tcgcacagca caccgcagtg accgtgagaa
     4201 gtgtattcac agaaacaagt gtctgacatg acgatcattt ctggcacagt ctgcttgcag
     4261 atgcgcgaca tacgcgccac cagtccatct tcccgccagg cgtcgctgcc ggtttcatcg
     4321 gtatggtgag agatgccgaa agtcatcacg gaacgaatac cggcgttggc gatgcgttca
     4381 atttcgcgtg ccagatgttt ctctggaatg cgcatcacgc ctggcatggc ttcaacggct
     4441 ttgtagtcgt caatttcttc ttcaacaaag atcggcaaca ccaggtcgtt aaggctaagt
     4501 gttgtctctt caaacatagc gcgcagcgca ggagatttgc gcaggcgacg agggcgttgg
     4561 attaagtctg tcatggtctg cctgatgttt gtggaattaa ggtgcacagt atacctgaag
     4621 cggggcagga gtgttttacg aaagttgtcg tgatgttgac aagaagagaa tgaaagagag
     4681 accaggacat ctttgtaata gaaatagcaa
//
LOCUS       DAJVUJ010000225         4650 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.227, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000225 DAJVUJ010000000
VERSION     DAJVUJ010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4650
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.227"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(<1..753)
                     /locus_tag="OQQ48_003952"
     CDS             complement(<1..753)
                     /locus_tag="OQQ48_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CSS-motif domain-containing protein"
                     /protein_id="HCS1954532.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLQQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLL"
     gene            complement(884..2248)
                     /gene="sdaA"
                     /locus_tag="OQQ48_003953"
     CDS             complement(884..2248)
                     /gene="sdaA"
                     /locus_tag="OQQ48_003953"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCS1954533.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(2432..3010)
                     /locus_tag="OQQ48_003954"
     CDS             complement(2432..3010)
                     /locus_tag="OQQ48_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HCS1954534.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVIGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            complement(3014..4375)
                     /gene="pabB"
                     /locus_tag="OQQ48_003955"
     CDS             complement(3014..4375)
                     /gene="pabB"
                     /locus_tag="OQQ48_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HCS1954535.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVAEPICTLTTFGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVTKILRQ
                     LEK"
     gene            4449..4628
                     /locus_tag="OQQ48_003956"
     CDS             4449..4628
                     /locus_tag="OQQ48_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HCS1954536.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
BASE COUNT         1166 a         1197 c         1209 g         1078 t
ORIGIN      
        1 tagcagcacc gccagcggta attgtgttgg aagatagtgc catgccagcg ccgtagcccc
       61 cggaccatta acgttaatgg taaaaggaaa gcgttgcgaa gaaacctgat agttttcatt
      121 gtcttcaggt tgcggaaggg aatctaccag cccattacca tagagcaaat gccgtttgtc
      181 caccgtcagg ctggcgctac tgatttgctg cagttgtggt tcaaggagca ttgccgtcag
      241 taagtcgatg ttaatcatct ccatgacccc agcattgcta ctgcccgctg caggcgtcca
      301 ttgaatcaaa accggactgc ctttaatcag ggcacggtcg atcgtcaggc gtaaaagtgg
      361 ttgcggtgca ggaagttcag ccagaatgtc cacgacgggc acattgcgat aaccaaaaat
      421 gctggagcaa tagagtgtgc cgtcttgcac caggccaatg gatcgcacag tttggagttt
      481 tgccgcctgt ttacgtaatg gcaaatgggc gacagagcag ggcagaccaa tcagcggaag
      541 caagacttcg ctacctgcct gtaggggaag cagtacttta tctaattcct ctacagcgtg
      601 attggcgaat tgtactaccc gttgttgatt taagttacgc tgtgaaataa atcgcacgct
      661 cagggtcgaa gcgagcgtaa cgagtgcgca aatcgtacaa acaatcattc gattacgtcg
      721 ataggtttta atgatccgtt gtgctttttg catgcgggcc accctgtaag cgtatggcga
      781 caaacgcctg acgcaacagt ggatgtgtag tggggaatat tacagcagac gagaaagcgg
      841 gtataaattc gcccatccgt tgcagatggg cgagtaagaa gtattagtca cactggactt
      901 tgattgccag accaccgcgt gaggtttcgc ggtatttggc gttcatgtcc ttaccggttt
      961 cgtacatcgt ttcgatgacc ttatccagcg agacgcgcgg tgcactggtg cggcgcagag
     1021 ccatccgtgc ggcgttaatc gccttcacag aggcaatggc attacgctca atgcacggca
     1081 cctgaacctg ccctgcaacc gggtcgcagg ttaaaccaag gttgtgttcc atgccaattt
     1141 ccgccgccac gcaaacctgt tccgggctac cgcccagcag ttctgcaaga cccgcagcag
     1201 ccattgaaca ggcaacaccc acttcgccct ggcaaccaac ttccgcaccg gaaatagagg
     1261 cgttcatttt atacaatgca ccaatcgcgc ccgctgccat aaagtaacgg gtatagatgt
     1321 ccgggctgac cgattcaata aagtggtcat agtaagccag cactgccgga acgataccgc
     1381 aggcaccgtt ggttggcgca gttaccacac gaccaccggc ggcgttttct tcgttaactg
     1441 ccagcgcaaa catgtttacc cagtcaatga cattcatcgg atcgttagac agtttatcgc
     1501 tggaaaccag catccggcgc agggcagacg cacgacgtgg cacgcgcagc gggcctggca
     1561 gtacaccttc ggtgttcatc ccgcgatcga tacatgcctg catggtttgc cagacatgcg
     1621 cgaaatactc gtcgatctct ttcttgctgt gcagcgccag ttcgttctgc atagcgagac
     1681 cagacagcga atagccggtt tcattacagt acgcgagcag ttcggtggca gatttgaacg
     1741 gatacggcac gctcacttcg ttggcagcat cctgaccaaa gtgttcttca tcgacgataa
     1801 aaccgccgcc gatggaataa taagttttgc tgtagacgac ttcatcgccg ttataggcgt
     1861 ggatttgcat accgttttca tgcagcggca ggttgccgtt atgaaaacgc atcccgttgt
     1921 cgcgcgggaa atccacttca tgccgtccct gtgccagcag cagacgttcg cgctcttcta
     1981 cgtcgcgaat aaaaccggga atactgtcga tatccacggt ggcaggttcg ttacctgcaa
     2041 gacccataat aatggcgata tcggtgtggt ggcctttacc cgtcagcgac agtgaaccat
     2101 aaacgtccac ggcaacgcga gtaacgctat ccagtaagcc tttttcgacc agatcatcga
     2161 cgaactgttt acccgccttc ataggcccta cggtatggga agatgaggga ccaatcccca
     2221 ccttaaacat gtcgaataga ctaatcacga taatactcct gacaaggtga ctggacttcc
     2281 agtaacgaac taataacagc gcatagtgta agagggaacg cggcgactgg cttaactatt
     2341 cacatgaatt aaactaatca aaaaccgcga tttaactaat attgcgatga ttgtctcaag
     2401 tagtgttttg tagatgtaaa gataagtata gtcagggttt cacaccaatt tgcagcgcca
     2461 gctcacgaat tatgcctgcg gtcattcccc atacaaaata ctgttcgtac caggacagcc
     2521 atacccgatg tgaatcgcca cggcggtaga tatctaaagg gtgataacga cccagatgta
     2581 atgcctgggc gagcggcatt tcaaacaccg ccgagacttc atcttcactg gcgcgatacg
     2641 gcagatcggg cgggataatg ccgatcactg gggttacctg atagccagtt acgctatcga
     2701 cgggcggcag cacgccgata acttcaacgg cggaaggcgg tatagcgacc tcttcttcag
     2761 cttcgcgcag cgcggcggcg ataactgatg cgtccgtgtc atcgactgca cctccaggga
     2821 atgccacttg tccagcgtgt ttacgcagat gaattgaacg ctgagtcagc aacaaccccg
     2881 gttgcggtcg acggacgatg gggattaaca cggcagcctg acgatgattt agggtttccc
     2941 ggttaatttg cgggcgcaaa agttgaaagc gagataaaaa atcatcaagc gtcaggctac
     3001 ggtattccac gtcttacttc tccagttggc gtaatatctt agtaacttta tcaaaagttt
     3061 cctgatattc cgcttcttcc tggctatcgg cgacaattcc accgcccgca gagcagtata
     3121 tttgtccgtt aatggcagtc agcgtgcgga tagtaatgct ggtatccatg ttgccgcaaa
     3181 agctcaaata gccaatgctg ccgcaccagg cattacgtcg ctgcggttcc agttcgtcga
     3241 taatttccat agcccgtact ttcggagccc cggttattga gccaccagga aaagctgcgc
     3301 gcagcagatc gctggcgtgt aactgttctg gtagtcgcgc cgttatagtg ctgaccagat
     3361 gatgcacggc agggaagggt tccaccacga agagctctgg tacttttacc gaaccggcta
     3421 cggcaacacg accgatatca ttacgcatta aatcgacaat catcagattt tcggcacgat
     3481 ctttcgctga gttcgccagt ttttctgctt gtttgctatc ttcctgagga tcgggcaggc
     3541 gtggtagcgt gcctttaatc gggcgggtct ggatttcact attatcacaa agaataaacc
     3601 gctctggcga aaggcttaaa attgcaccct gttcaagacg taaaaaagcg ctaaatggcg
     3661 cgcggttggc ctgattaagc tgaaggaatg cctgccattc atcgccagaa taggtcgcat
     3721 gaaaacgctg ggcgagattc acctgatagc aatcaccgct gtgcagatat tcctgtacct
     3781 ggcgaaattt ttcgccgtac tgctcgcggg tcatattgga ttgccagtcg gaagtgagcg
     3841 tgaaatcttc ctgcggcgag aattgctggc tttccagcca ggcccgacgg gcattgacat
     3901 cattatgact cagcaaagaa actgtatgac gctggtggtc gacaatgagc gcccaatcgt
     3961 agatacccac tgccatatcc ggcagaacga tatcttgttc cgcaatttct ggcagtgact
     4021 caaaacggcg gcccagatcg tagccaaaca accccagtgc gccgccctga aatggcaaat
     4081 cttcgttatg cgttgggcga atgtctgcgc gatccagcac ctgctggagc acctgtagcg
     4141 ggtcatcagt ggtcgttgtg cgtttttcgc tttcactaac aacggtttct ttaccgaaag
     4201 tggttaaagt gcaaatcggc tcggcgacca caatatcaaa gcggctatac ggatgatcgg
     4261 catagccgga gtgtaaaagc atcgcccacg gcaggtggct taagcgggag aaataaaatt
     4321 cagcggcgtc ctgacgccag ggtaaagtaa tcacagcggg agataacgtc ttcatcagtc
     4381 ctgactctac tggctatgtg ccgggtagca tgaaataatt agcgccaaca atttagcagg
     4441 agttaacaat gtttgcaggt ttaccttcac tcacccatga acagcagcaa aaagctgtcg
     4501 agcggatcca ggaactgatg gcacaaggga tgagcagcgg ccaggcaatc gcgctggtgg
     4561 cagaagagct gcgtgccaac cactccggcg agcgaatcgt ggcgcgtttt gaggatgaag
     4621 acgagtaaca cactaatccg tatcgatttc
//
LOCUS       DAJVUJ010000226         4587 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.228, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000226 DAJVUJ010000000
VERSION     DAJVUJ010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4587
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.228"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            253..615
                     /gene="queD"
                     /locus_tag="OQQ48_003957"
     CDS             253..615
                     /gene="queD"
                     /locus_tag="OQQ48_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HCS1954537.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            693..1961
                     /locus_tag="OQQ48_003958"
     CDS             693..1961
                     /locus_tag="OQQ48_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCS1954538.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGVTTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRPLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWQLVGDALRSCVNTGISVRGMGMALTGAQAAQTLISACQHREP
                     QNLFALYHHNVERSLLWDVLQRYQHVPALLQRPSWYRAWPALMQDISRDLWDQGDKPV
                     PPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            1952..2212
                     /locus_tag="OQQ48_003959"
     CDS             1952..2212
                     /locus_tag="OQQ48_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCS1954539.1"
                     /translation="MSVAHNLWRVADAPHIVPADSVERQMVQWLINACPAGLFSLTPE
                     GDLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            2229..2804
                     /locus_tag="OQQ48_003960"
     CDS             2229..2804
                     /locus_tag="OQQ48_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HCS1954540.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDACNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(2952..3812)
                     /locus_tag="OQQ48_003961"
     CDS             complement(2952..3812)
                     /locus_tag="OQQ48_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HCS1954541.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGIQQLNIVPGALPDWLVSTEDLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(3809..>4587)
                     /locus_tag="OQQ48_003962"
     CDS             complement(3809..>4587)
                     /locus_tag="OQQ48_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HCS1954542.1"
                     /translation="NILLAFKAEPDAGMLAEKEWQAAAQGKSGPDISLLRSLLGADEQ
                     AAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPEF
                     VARHIAEWQHQNPLDLIITGSQSSEGQNGQTPFLLAEMLGWTCFTQVERFTLDALFIT
                     LEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPAM
                     QCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
BASE COUNT         1056 a         1235 c         1164 g         1132 t
ORIGIN      
        1 agataaggtt aacggggtga acggtgtgga ttaaagacgg gatagcgata actaataacc
       61 aaatcgacta acctgtttta gcaatagtct ttaacaacaa aatagattaa ccaacccaat
      121 gaaaaacaaa tgaatttagc caatcattaa gataaatcag cgattttgcg caacaaagtc
      181 gttttagata atgcgaaaaa acagcctttc cggtactcta cggcggtttt atcgtcctgt
      241 agagaaatta tgatgtccac cacgttattt aaagatttca ccttcgaagc cgctcaccgc
      301 ttaccacacg tcccggaagg gcataaatgt ggtcgcctgc acgggcattc ctttatggtg
      361 cgactggaaa ttaccgggga agtcgatccg catacgggct ggattatcga tttcgctgaa
      421 ctaaaagcgg cgtttaagcc aacttatgag cgcctcgatc accattatct caatgatatt
      481 ccaggtctgg aaaacccaac tagcgaagtt ttagcaaaat ggatttggga tcaggttaaa
      541 cccgttgtgc cgctgttaag tgcggtgatg gtaaaagaaa cctgcaccgc tggttgtatc
      601 tatcgcggcg aatgataaga gtgtgtcggc ggtcaatttc ccttaagtaa cgctatgtta
      661 gggtattgtg ttcaggatat ctggggcatg acatggaaga cgactgcgac attattatta
      721 ttggtgccgg tattgcaggc accgcttgcg cgttacgctg cgcgcgagcg ggtttatccg
      781 ttttgttact ggaacgcgct gaaatccccg gcagcaaaaa tctttccggc ggacggttat
      841 atacccatgc actcgcggaa ctcctccctc agtttcatct gaccgcgcct cttgaacgac
      901 gcatcactca cgaaagcctt tccctgttaa cgcccgatgg cgtaacgacg ttttccagct
      961 tacagcccgg cggtgaatcc tggagtgtat tacgtgcacg gttcgatccg tggctggttg
     1021 ccgaagccga aaaagaaggt gtcgaatgta tccccggagc gacggtggat gcactgtatg
     1081 aagaaaacgg cagagtctgt ggcgttattt gtggtgacga tattctccgc gcccgttatg
     1141 tggtgctggc agaaggtgcc aacagcgtcc tggctgaacg tcacgggtta gtgactcgtc
     1201 ctgctggcga agcgatggcg ttggggatca aagaagtgct gtcgctggaa acatccgcta
     1261 ttgaagaacg ttttcatctg gagaataacg aaggcgcagc gttgctgttc agcggcggaa
     1321 tctgtgatga cttacccggc ggcgcatttc tttatactaa tcaacaaacg ctctcgttag
     1381 ggattgtttg cccgctctct tcccttacgc aaagtcgtgt tccggcaagc gagctgctga
     1441 ctcgctttaa agcgcatccg gcagtgcgcc cgcttatcaa aaacacggaa tcactggagt
     1501 atggtgcgca tctggtgcca gaaggtggct tgcacagtat gccggtgcaa tacgccggta
     1561 acggctggca gctggtgggc gatgcgttgc gcagttgcgt caataccgga atttccgtgc
     1621 gcggcatggg tatggcgctg actggcgcac aggcggcaca aacgctgata agcgcctgcc
     1681 agcaccgcga gccgcaaaat ctgtttgcgc tttatcatca caacgtagag cgcagcctgc
     1741 tatgggatgt tctacaacgt tatcagcatg ttccggcgct tttgcaacgc cctagctggt
     1801 atcgggcgtg gcctgcgtta atgcaggata tttcccgcga tttatgggat cagggtgata
     1861 aacctgttcc accgctgcgc cagttattct ggcgtcattt acgtcgtcat ggcctgtggc
     1921 atctggcggg cgatgttatc aggagtctgc gatgtctgta gcccataatc tctggcgcgt
     1981 tgctgatgcg ccgcacattg ttccggctga ctccgttgag cgtcagatgg tacaatggtt
     2041 gattaacgcc tgtccggcag gtcttttttc gctcacaccg gaaggtgact tacgcgttga
     2101 ttatcacggc tgcctggagt gtggcacctg ccgtttgctg tgcgacgaat ccacactaca
     2161 acagtggcgc tatccgcctt ccggattcgg catcacctac cgctttggat aaataataag
     2221 gataatttat gcccctctta cacctgctcc gccagaatcc ggtgattgct gccgttaaag
     2281 ataatgccag cctgcaactg gcaatcgact ctgaatgcca gtttatttcc gtgttgtacg
     2341 gcaatatctg caccatcagt aatatcgtta aaaagattaa gaacgccggg aaatatgctt
     2401 ttattcatgt tgatttgctg gaaggcgcgt caaataaaga agtcgtgatt cagtttttga
     2461 aactggtgac cgaggcggac ggcattatca gtaccaaagc ctcaatgttg aaagcggcaa
     2521 gagcggaagg ttttttctgt attcatcgcc tgtttattgt tgattcaatt tcgtttcaca
     2581 acattgataa gcaagttgcg caatcgaatc cggattgtat tgagatcctg ccaggctgta
     2641 tgcccaaagt gctgggctgg gtgacagaga aaatccgcca accgctgatt gccggtgggc
     2701 tggtgtgcga tgaagaagat gcgtgtaatg cgattaacgc gggtgtcgtg gcgctttcca
     2761 ccacgaatac cggggtctgg acgttagcga aaaaattact ttgacgggat aatcgtaacc
     2821 aattgaattt ggtttgattt gtaggccgca cgccacatcc gacattcagc gcctgatgcg
     2881 acgcttgacg cgtcttatca ggcctacaag tccccaggcc agaaccgtag gcaggatcat
     2941 ccggcaacac attattggca atctgcgtga atgtttgtca ccagtgcctc cagcaccact
     3001 ttccagtcat caaccacgcc aacatctgcc tgtgaaaata ccgcagcgct ggcgtcatga
     3061 ttaatcgcca ccacaaactt gctattgcgt acgcccgcca ttaacgctgc tgcgccggaa
     3121 gcgccaacaa caatacacac ctcaggtgcc agcaaatgtc cggaaatacc aatcactttt
     3181 tcagcatcaa cgccaccgtt catcactcgc gcccgactgt agcccacttc ggctcccagc
     3241 ttttctgcca gcatagcgat ttcctggtta tctgcctctc cgccctgccc caccaccagc
     3301 acccgtctgg cctcagctaa tgggtcgcga gtaacatttt tcagatcctc tgtgctaacc
     3361 agccagtcgg gaagcgcacc cgggacaatg ttcagttgtt gtattccaga gggcaacgtg
     3421 gcgtttttcg ctgctcccgc ctgacgcgcc agagaaaggc atagcgggcg tttttctgtt
     3481 tgcagcgtcg ccgttagcgc atttccccag tgggatttgc gcacgcttac ggttggaata
     3541 tctaacgaag tcacctggca gatgcttgca ccatgtaaac gccaggccag ccgggtcgac
     3601 agttcatcgc caaacgttcc cggcggaaag agaacaacgt ccgcaggcgt tcgctgccac
     3661 tgttccacca acgcatccag cacctgttcc gccactacag gctgaggttc aatctgccag
     3721 tgcgccaggg tacaaccact gaaatcctgc gccgccagcc agcttgctat tgcggcattc
     3781 tcctgattta ttgtcacaat tgcgatattc atggctgcat cctttggcgt aaatagtcct
     3841 gccatagctt ttgagctttt tctgctaccg tttgcccatc aatcagcgtc gctccgcgtc
     3901 gttgctctgc tcttgccagt tgcagacact gcatcgctgg catttcggca gcaacagttt
     3961 tacggataat ttctgctttc cccgcagcca ttcgctggcg cattcccggt actggcaagg
     4021 ccacttcacc gcactgacgc acggcaatca ccgcaggcaa gcgaacccgg caacaacgca
     4081 acccatgttc agtacgctgt tcgagggtga taaacagcgc gtcgagagtg aaacgctcca
     4141 cctgggtgaa gcaggtccag cccagcattt ccgccagcaa aaatggcgtt tgcccattct
     4201 gcccttcgct gctttggctg ccggtaatta tcagatccag cggattctga tgctgccatt
     4261 cggcaatatg gcgagcgaca aattccggcg caaagcgcag atctgccgcc gtctccagca
     4321 atacagcttc ctcaaaccca agagccatga gatagcgcag ccagtgcaat gcccgttcat
     4381 cccccatgct caacgcggtt aaagacatcg gcgtaccgtt ttttctctgc gccaacagca
     4441 gcgcggcagc ggcctgttca tcagcaccga gtaaacttcg cagtagcgaa atatccggtc
     4501 cgcttttacc ctgagccgcc gcctgccact ctttttccgc cagcattccg gcatccggtt
     4561 cggctttaaa cgctaacaga atgttca
//
LOCUS       DAJVUJ010000227         4521 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.229, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000227 DAJVUJ010000000
VERSION     DAJVUJ010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4521
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.229"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(125..1039)
                     /gene="nrdF"
                     /locus_tag="OQQ48_003963"
     CDS             complement(125..1039)
                     /gene="nrdF"
                     /locus_tag="OQQ48_003963"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCS1954543.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPYALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYSDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSRIGN"
     gene            complement(1049..3193)
                     /gene="nrdE"
                     /locus_tag="OQQ48_003964"
     CDS             complement(1049..3193)
                     /gene="nrdE"
                     /locus_tag="OQQ48_003964"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCS1954544.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAYAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDATLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPVAGRINMSNLCSEIL
                     QVNCASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYAITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            complement(3166..3576)
                     /gene="nrdI"
                     /locus_tag="OQQ48_003965"
     CDS             complement(3166..3576)
                     /gene="nrdI"
                     /locus_tag="OQQ48_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCS1954545.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGMPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            complement(3573..3818)
                     /gene="nrdH"
                     /locus_tag="OQQ48_003966"
     CDS             complement(3573..3818)
                     /gene="nrdH"
                     /locus_tag="OQQ48_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCS1954546.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            complement(4066..4395)
                     /locus_tag="OQQ48_003967"
     CDS             complement(4066..4395)
                     /locus_tag="OQQ48_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCS1954547.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
BASE COUNT         1111 a         1144 c         1158 g         1108 t
ORIGIN      
        1 agtataaaaa acagcctgtt gcggtataag aatcgaccat gcagagagag aaaacaccgg
       61 agtggaggga aaagcagaaa agctcacgtg gtataaggcg cggacagggg tatcgcttag
      121 tttttcaatt tcctatacgt gaaccggaaa agaaatcgtg attttcatcg gcattcggcg
      181 aaagcgcggc gaggattgcc ggattcactt ccgccatttc tgcgggaaat aacggttcgt
      241 agcccagatt catcaaagcc ttattggcgt tgtaacagag aaacgctttc acatcgtcag
      301 cccacggggt ttcggcgtac agctcatcgc tgtattgcaa ctcgttgtcg tagagttcca
      361 gcaacaaatc gaaggcgaaa ctcttcaact cttcacgttg tcccagagat atcttttcca
      421 tgtttttctg atatttatag cctatgtagt aaccgtggac tgcttcatcg cggataatca
      481 gacggatcag atctgcggta ttggtcagct ttccgcggct ggaaaaatac atcggcagcc
      541 agaaaccgga atagaacaaa aaagattcaa gaaacacact ggcgattttc tttttcagcg
      601 gatcatcacc gcgataatgt tgctgaataa tctgagcttt tcgctgcaac ggtgcgtttt
      661 cttcactcca ggcgtaggcg gcatcgacat ctttggtctg acacagcgtc gagaaaatcg
      721 aactgtaaga gcgggcatga accgcttcca taaagctgat attcgataat accgcttctt
      781 catgaggcgt gagtgcatag ggcatcagcg aaggcgcgcc gataacattt tgcagcgtgt
      841 cgagcagcgt caggccagta aaaacgcgca tcgtcagttg ttgttctacg acagttaatg
      901 tctgccaggc aggaatatcg ttcgacagcg gcaccttttc tggtagccag aaattgctgg
      961 tcaggcgatt ccacacctcc agatctttat cgtcagatat cttgttccag ttgatggcgc
     1021 tgatacgtga gagtttcata gatattcctt aaagtgcaca ggagacgcag ccttcaattt
     1081 cagtgccttc cagcgccatc tgacgcaggc gaatgtaata gagcgttttg atacccttgc
     1141 gccaggcgta aatctgcgct ttgttgatat cgcgagtggt ggcggtatcg gggaaaaaca
     1201 gcgtcagcga cagcccctga tcgacatggc gagtcgcttc ggcgtaggtg tcgatgatct
     1261 tttctgcgcc gatttcgtaa gcatcctgat acagcgccag attctcgtta gtcataaacg
     1321 gggcagggta gtaaacgcgt cctgttttgc cctctttgcg tatctctact ttcgccacaa
     1381 tcggatgaat actcgacgta gcatggttga tataagagat agaaccggtt ggcggcaccg
     1441 cctgaagatt ctggttataa atgccgtagc gcatcacgtc gtcgcgcagt tgcgcccaca
     1501 tctcacgggt aggtaacgta ataccgctgc gggcaaacag ttcgccaact ttcgccgttt
     1561 tcggctgcca gttaccttgc agatattggc taaaatattc accactggca taacgtgact
     1621 gtttgaaccc ggcgaaggtt tcaccgcgtt cgcgcgccag caacatcgag gtacgcagtg
     1681 cgtgccaggt gatggcatag aaatagagat tggtgaaatc cagtgcttcc ggcgaaccat
     1741 aagcgatgcc ttctcgcgcc agatagccgt gtaaattcat ctgccccagt ccgatggcgt
     1801 gtgaggcggc atttccggct tcgatggacg gcacgctgcg gatatgactc atatctgaca
     1861 ccgccgttaa accgcgcacg gcagtctcta ccgtgcgggc aaaatcgggg gaatccatgg
     1921 tgtgcgcaat attcaacgaa cctaaattgc aggagatatc atggcctgtg cgggcatagt
     1981 cgagattctc gtcatactct gaggcacagt tgacctgcaa aatctctgag cagagattac
     2041 tcatatttat gcgcccggcg acagggttag cacggtttac cgtgtcttca tacatgatgt
     2101 agggatagcc ggactcaaac tggatttctg ccagccgctg gaagaaatca cgggcgttga
     2161 ggtatttttt gcggatgcgt tcatcggcaa ccagttcgtc atagtgttcg ctgatggcga
     2221 tatcggcaaa cggcttgcca taaactcgct caacgtcata gggcgaaaac agtgccatct
     2281 gcgcattctc ttttgccaga tggaaagtga tatccgggat caccacgccc agcgacagtg
     2341 ttttaatgcg gattttttcg tcggcatttt cccgttttgt atcgagaaaa cgcagaatat
     2401 cgggatgatg agcatgtaaa tagactgcac ctgccccctg acgcgcgccg agttggttgg
     2461 cataggaaaa tgcgtcttcc agcattttca tcaccggaat tacgccagaa gattgatttt
     2521 caatacgttt aattggcgcg cccgcttctc gcagattcga cagcaaaaat gccacgccgc
     2581 cgccgcgttt cgacagttgc agtgcggaat ttaccgcccg gccaatcgac tccatattgt
     2641 cttcaatacg cagcagaaaa caggaaacca gttcgccgcg ctgctgctta ccgcagttga
     2701 ggaatgtcgg agtggctggc tgaaagcgtc ctgacagcat ttcatcggtc agttgcaacg
     2761 ccagcgtcgc atcgccttgt gccagcgtca gcgccaccat cgtgactcga tcggcaaaat
     2821 cttccagata acgtttaccg tcgaatgtct tcaacgtata gctggtgtaa aacttccatg
     2881 ccccgaggaa tgtctggaaa cgaaaaccgc tggtgtgtgc gtaggcaaac agcgtaatga
     2941 caaagtcgcg agagtagcga ttaagaacgc tttcatcata gtaaccttcg ttgaccagcc
     3001 agttcaggcg ctgctgctgg ctactgaagg tcacactgtt cggacgcaca tgcgtcgcca
     3061 taaaggcgtc aacggcctgg cggtctttat cgaactgaat gcgacctgcg ctatcgtaga
     3121 ggttaagcat cgcattcagc gcgtggtaat ccatcgtttc ctgcgtcagg cattctgcgg
     3181 ttgtcgttgc caaaattcgg ttactccttt acgaacgttt tcgatatcgc tttgcgtacc
     3241 catgagttca aaacggtaca gccacggcac gccgcatttc cgggcaatca catctccggc
     3301 gcggccatac gcctcaccaa agttgcgatt accagaagca ataacgccgc gaagcaacgc
     3361 ccggttgtgc tcgtcgttta aaaagcgaat tacctgtcgt ggcaccgcgc cagccgtacc
     3421 gccgccgccg taagagggca cgatcaggat gtaaggctcg tctacctgaa tccgttcccg
     3481 ctcattgagc gggatgcgca ccgcgggcat acctaaacgt tcgataaaac gctgcgtgtt
     3541 ttcggagctg ctggagaagt agacgagctg gctcatgcac tggccgcgtg tggcgctgga
     3601 tgcagacggt taatcatgtc cggacggaaa ccagaccagc taagatcgcc agcaatcact
     3661 accggcaact gacgaaagcc ctgagcacgc aacgcttccg ctgcttcagg aacgcgatcg
     3721 acattaatca tttcaaaatc aaacccccgg ttttccatcg cccgtttggt ggcgtggcac
     3781 tgaacgcaat cgttacgagt gtaaatagtt atgcgcatga ttcgtatttc cgtttaaaat
     3841 gaagatacgg cgcgatgata cgcgtcgggt tgtctctctg ttgatacaga gatactagat
     3901 gtagttgaaa aaagattcaa ctacacaata tatagcaaat agtgatcgaa attaccctgg
     3961 atatgagcgt gacgggggag ggggattttt gtgattcacc aggcaaaaag aaaccccgaa
     4021 gacaggcttc ggggtcaaag acgcgtattt attatcattt ttgcactacg atttgcgcat
     4081 gcttaacagt gcgccgataa aaatacctac cgcagctgct gtacccacgc tacaccaggg
     4141 tctttcacga acaaaagaat ccgcgcagcc aacggcatcg cgcgcggctt gctggacgcg
     4201 agtacgacca tgcattcgcg ccctggtttc tttcagcaac gcctgagctt tactgcgtgc
     4261 ggcttcagct tcccctttgg cgtcgctgcc ccaggacttc aatacagatt cgaggctgtc
     4321 agctaattga ttgacatcat tctggatatc ctgcacgcca tcatcaacgt cgttgcggtt
     4381 cggtcggtta aacatatgat cccccattag aattcgatgt aattttcagt gtagaccata
     4441 attttcttaa ctgaggttca tcgcggcttt atggactttt ctgaaagcgt tgtgaatgcg
     4501 caatcaggta aggtaggccc g
//
LOCUS       DAJVUJ010000228         4420 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.230, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000228 DAJVUJ010000000
VERSION     DAJVUJ010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4420
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.230"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            127..1926
                     /gene="cysJ"
                     /locus_tag="OQQ48_003968"
     CDS             127..1926
                     /gene="cysJ"
                     /locus_tag="OQQ48_003968"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HCS1954548.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKD
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDIEAADEFLSELRVERRYQRDVY"
     gene            1926..3638
                     /gene="cysI"
                     /locus_tag="OQQ48_003969"
     CDS             1926..3638
                     /gene="cysI"
                     /locus_tag="OQQ48_003969"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HCS1954549.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPHHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            3713..>4420
                     /gene="cysH"
                     /locus_tag="OQQ48_003970"
     CDS             3713..>4420
                     /gene="cysH"
                     /locus_tag="OQQ48_003970"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HCS1954550.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMSEEETRFFGLK"
BASE COUNT         1085 a         1172 c         1259 g          904 t
ORIGIN      
        1 aatcggaaaa tcgatactgg aaaaactcta actcggtggc cagtttttgt tgaccacttc
       61 actcttctgt tttatgggcg ctgacagggc gcagaaacag ctttgcttac tggaacataa
      121 cgacgcatga cgacacaggt cccaccttcc gcgttgcttc cgttgaaccc ggagcaactg
      181 gcacgccttc aggcggccac gaccgattta acgcctaccc agcttgcctg ggtttctggc
      241 tatttctggg gcgtactcaa tcagcagcct gctgcgcttg cagcgacgcc agcgccagcc
      301 gcagaaatgc cgggtataac tattatctcc gcctcgcaaa ccggcaatgc gcgccgggtt
      361 gctgaagcat tacgcgatga tttattagca gcaaaactga acgttaagct ggtgaacgcg
      421 ggcgactata aattcaaaca aatcgccagc gaaaaactgc tcatcgtagt gacgtcaacg
      481 caaggggaag gggaaccgcc ggaagaagcc gtcgcgctgc ataagttcct gttctccaaa
      541 aaagcgccaa agctggaaaa caccgcgttt gccgtgttta gcctcggcga tagctcttat
      601 gaatttttct gccagtccgg gaaagatttc gacagcaagc tggcggaact gggtggtgaa
      661 cgcctgctcg accgtgtcga tgccgatgtt gaataccagg ctgctgccag cgagtggcgc
      721 gcccgcgtgg ttgatgcgct taaatcgcgt gcgcctgtcg cggcaccttc gcaatccgtc
      781 gctactggcg cggtaaatga aatccacacc agcccgtaca gcaaagacgc gccgctggtg
      841 gctagcctct ctgttaacca gaaaattacc gggcgtaact ctgaaaaaga cgttcgccat
      901 atcgaaattg acttaggtga ctcgggcctg cgttaccagc cgggtgacgc gctgggcgtc
      961 tggtatcaga acgatccggc actggtgaaa gaacttgtcg aactgctgtg gctgaaaggc
     1021 gatgaacctg tcaccgtcga gggcaaaacg ttgcctctga acgaagcgct acagtggcac
     1081 ttcgaactga ccgtcaacac cgccaacatt gttgagaatt acgccacgct tacccgcagc
     1141 gaaacactgc tgccgctggt gggcgataaa gcgaagttac agcattacgc cgcgacgacg
     1201 ccgattgtcg acatggtgcg tttctctccg gcgcaactgg atgccgaagc gctgattaat
     1261 ctgctgcgcc cgctgacccc gcgcctgtat tccatcgcct cctcgcaggc ggaagtcgag
     1321 aacgaagtac acgtcaccgt tggtgtggtg cgttacgacg tggaaggccg agcccgtgcc
     1381 ggtggtgcct ccagcttcct cgcggatcgc gtggaagaag agggcgaagt ccgcgtattt
     1441 atcgaacata acgataactt tcgcctgccc gctaacccgg aaaccccggt gattatgatt
     1501 ggcccaggca ccggtattgc gccgttccgc gcctttatgc agcaacgcgc cgccgacgaa
     1561 gcgccaggta aaaactggct gttctttggt aatccgcact ttacggaaga cttcctgtac
     1621 caggtggagt ggcagcgcta cgtcaaagat ggcgtgctga cacgtatcga tcttgcctgg
     1681 tcgcgcgacc aaaaagaaaa agtttacgta caagacaaac tgcgcgaaca gggcgcggag
     1741 ctgtggcgct ggatcaatga tggtgcccac atttatgtct gcggcgacgc taatcgcatg
     1801 gcgaaagacg ttgagcaggc acttctggaa gtgattgccg aatttggtgg catggacatc
     1861 gaagcggcgg atgaattttt aagtgagctg cgcgtagagc gccgttatca gcgagatgtc
     1921 tactaatgag cgaaaaacat ccagggcctt tagtggtcga aggaaaactg acagacgccg
     1981 agcgcatgaa gcttgaaagc aactacctgc gcggcaccat tgcggaagat ttaaacgacg
     2041 gtctgaccgg cggctttaag ggcgacaact tcctgctgat ccgcttccac ggcatgtatc
     2101 agcaggatga ccgcgacatc cgcgccgaac gtgctgaaca gaagctggag ccgcaccacg
     2161 cgatgctgct tcgctgtcgt ctgccgggtg gggtgattac cactaaacag tggcaggcga
     2221 tcgacaaatt tgccggtgaa aacaccatct atggcagcat tcgcctgacc aaccgccaga
     2281 cattccagtt ccacggcatt ctgaaaaaga acgtcaaacc ggtgcaccag atgctgcact
     2341 cggtcggtct tgatgcgctg gcgacagcta acgacatgaa ccgtaacgta ctctgcacct
     2401 cgaaccctta cgagtcgcag ctgcacgcgg aagcgtacga gtgggcgaag aagatttctg
     2461 agcatctgtt gcctcgtacc cgcgcgtatg cggagatctg gctcgaccag gaaaaagtcg
     2521 ccactactga tgaagaaccg atccttggtc agacctacct gccgcgtaag ttcaaaacca
     2581 cggtagtgat cccgccgcag aacgatatcg atctgcatgc caacgacatg aacttcgtgg
     2641 caatcgccga aaacggcaag ctggtaggct tcaacctgct ggtgggcggt gggctttcta
     2701 tcgaacacgg caacaagaaa acctacgccc gcacggcgag cgagtttggc tatctgccgc
     2761 tggagcatac gctggcggtg gccgaagctg tcgtgacaac tcagcgtgac tggggtaacc
     2821 gaaccgatcg taaaaatgcc aaaaccaaat acacgctgga gcgcgtgggg gttgagacgt
     2881 ttaaagcgga agtggagcgt cgcgcgggga tcaaatttga accgatccgt ccatatgagt
     2941 tcaccggacg aggcgatcgt attggctggg ttaagggcat tgatgataac tggcacctga
     3001 cgctgtttat cgaaaatggt cgcatccttg attatccggg gcgtccgctg aaaaccggcc
     3061 tgctggagat cgcgaagatc cacaaaggcg atttccgcat tacggcgaac cagaatctga
     3121 tcatcgccgg tgtaccggaa agcgagaaag cgaagatcga gaagatcgcc aaagagagcg
     3181 ggttaatgaa tgccgtcacg ccgcagcgtg aaaactcgat ggcctgcgtg tcattcccga
     3241 cttgcccgct ggcgatggcg gaagcagagc gtttcctgcc gtcttttatc gacaacatcg
     3301 ataatttaat ggcgaaacat ggtgtcagcg atgagcatat cgtgatgcgt gtaacaggct
     3361 gcccgaacgg ttgtggtcgc gcgatgctgg cggaagtggg cctggtgggt aaagcgccgg
     3421 gtcgctacaa cctgcatctt ggcggcaacc gcattgggac acgtatccca cggatgtata
     3481 aagaaaacat caccgagccg gaaatcctgg cgtcgcttga tgaactgata gggcgctggg
     3541 cgaaagagcg cgaagcgggt gaaggcttcg gcgactttac ggtgcgtgcg ggcatcattc
     3601 gcccggtgct cgatccggcg cgcgatttgt gggattaacc atcagcccgg tcttgtaggc
     3661 ctgataagaa cgcgtgagcg tcgcatcagg caaggcaaac agtgaggaat ctatgtccaa
     3721 actcgatcta aacgccctga acgaactgcc gaaggtagat cgcattctgg cgctggcgga
     3781 aactaacgcc gaactggaaa aactggacgc tgaaggccgc gtagcctggg cgctggataa
     3841 tctgcccggt gaatatgtac tttcttccag ctttggcatt caggcggcgg tgagcctgca
     3901 tctggtgaat caaattcgcc cggatattcc ggtgatcctc accgatacgg gttacttgtt
     3961 cccggaaacc taccgcttta ttgacgagtt aacggacaaa ctcaagctca acctgaaagt
     4021 gtaccgtgct accgaaagcg cagcctggca ggaagcacgc tacggaaaac tgtgggagca
     4081 gggcgttgaa ggcattgaaa agtacaatga catcaacaaa gtcgaaccga tgaaccgggc
     4141 tctgaaagaa cttaacgcgc aaacctggtt tgctggcctg cgccgtgaac aatccggcag
     4201 tcgcgccaat ttaccggtgc tggcaattca gcgtggcgta tttaaagtgc tgccgattat
     4261 cgactgggat aaccgaacta tttatcagta cctgcaaaaa catggcctga aatatcaccc
     4321 attatgggat gaaggatatt tatcggtcgg tgatacccat acaacccgta aatgggaacc
     4381 tggcatgtcg gaagaagaaa cacgtttctt tggcttaaaa
//
LOCUS       DAJVUJ010000229         4404 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.231, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000229 DAJVUJ010000000
VERSION     DAJVUJ010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4404
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.231"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            228..600
                     /locus_tag="OQQ48_003971"
                     /pseudo
     CDS             228..600
                     /locus_tag="OQQ48_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1162..2109)
                     /gene="icsP"
                     /locus_tag="OQQ48_003972"
     CDS             complement(1162..2109)
                     /gene="icsP"
                     /locus_tag="OQQ48_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease IcsP"
                     /protein_id="HCS1954551.1"
                     /translation="MKLKFLVLALCVPAIFTTHATTNYPLFIPDNISTDISLGSLSGK
                     TKERVYHPKEGGRKISQLDWKYSNATIVRGGIDWKLIPKVSFGVSGWTTLGNQKASMV
                     DKDWNNSNTPQVWTDQSWHPNTHLRDANEFELNLKGWLLNNLDYRLGLIAGYQESRYS
                     FNAMGGSYIYSENGGNRNKKGAHPSGERTIGYKQLFKIPYIGLTANYRHENFEFGAEL
                     KYSGWVRSSDTDKHYQTETIFKDEIKNQNYCSVAANIGYYVTPSAKFYIEGSRNYISN
                     KKGDTSLYEQSTNISGTIKNSASIEYIGFLTSAGIKYIF"
     gene            complement(3490..3642)
                     /locus_tag="OQQ48_003973"
                     /pseudo
     CDS             complement(3490..3642)
                     /locus_tag="OQQ48_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            3698..4390
                     /gene="ospZ"
                     /locus_tag="OQQ48_003974"
     CDS             3698..4390
                     /gene="ospZ"
                     /locus_tag="OQQ48_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase OspZ"
                     /protein_id="HCS1954552.1"
                     /translation="MISPIKNIKNVFPINTANTEYIVRNIYPRVEHGYFNESPNIYDK
                     KYISGITRSMAQLKIEEFINEKSRRLNYMKTMYSPCPEDFQPISRDEASIPEGSWLTV
                     ISGKRPMGQFSVDSLYHPDLHALCELPEISCKIFPKENSDFLYIIVVFRNDSPQGELR
                     ANRFIELYDIKREIMQVLRDESPELKSIKSEIIIAREMGELFSYASEEIDSYIKQMND
                     RFSQIKARMSVT"
BASE COUNT         1351 a          863 c          851 g         1339 t
ORIGIN      
        1 tgcgatctgg ttcaacaata tgcagatggt acacaacctc tttaaccagc ccatccggat
       61 tattcagagc ccttccgcaa tccggaataa aagtgctgtt ttaccgtgaa ccctctgaaa
      121 atcagcgagc agtgttttaa ccagccgacg ttcagagcgt actgacagac ggtggacaat
      181 ttgaataaac agttcaatca gaccgtagat gctctcccgt tggcgacatg ctgggagcgg
      241 tggaacaccg cttcggcaac gagcttccgg cgtctccagt gaagtgactg acggataatg
      301 gttcatgcta ccgggctaat gaaacacgcc agttcacccg gatgttggga ctttgttgaa
      361 ccgaagaaca cggtggtgcg gagtccggag agtaacagaa tagcaaagag cttcgtgaaa
      421 acaataaagt gtgactacat cagtatcatg cccaaacctg acgggttaac ggcagcaaag
      481 aaccttgcag aggcattcga gcattataac gaatggcatc cgcatagtgc gctggattat
      541 cgctcgccac gggaatatct gcggcagcgg gccaatgata acaggtgtct ggaaatatag
      601 gggcaaagcc acccacgaac gatccagcat ataatcaaga aattagattt catttaacat
      661 aagatagatt atgtgcacca agaggcattt ccttaatttc tgaatttaca ggctcttttg
      721 ctctctttgc tatgctgtgg atttggaaag atagagtgct ggctgattat ttaggtattt
      781 tcatcagcat gtccctgata gcactgttcc atcagcgctt tcaggcgata atagtagacc
      841 ttgggtagcc tctgctcatg tgtaacttga taccccgaga acagccacga tgctctaagc
      901 ggttcttatt tctccggtct cagggtcggt aatgccaaca gtaagtccac agtaatcaac
      961 gaagagtttt ttgaaccgcc ccggaaatcc tggagactaa acgccctgag aaagaggtaa
     1021 acagaatgac taaaaataca cgtttttccc ccgaagtccg tcaacgggca gttcgtatgg
     1081 ttctggaaag tcagggcgaa tatgactcac aatgggcggc aatttttagc catgaaaacg
     1141 taatcaactc ggtgaaccaa ttcaaaaaat atactttata cctgcggaag tgagaaaacc
     1201 aatatattca atacttgcac tatttttaat ggtgccagat atattggtac tttgctcata
     1261 aagagatgta tcaccttttt tattagaaat gtaatttctg gagccttcta tataaaattt
     1321 tgcactgggg gtgacatagt atccaatatt cgcagcaaca gagcagtaat tttggttttt
     1381 tatttcatct ttaaaaattg tctcagtctg atagtgttta tctgtatcag atgaacgaac
     1441 ccaaccacta tatttcagtt ctgctccaaa ctcaaaattc tcatggcggt aattagcagt
     1501 taatccaata taaggtattt tgaagagctg tttgtaacct attgttcttt caccactagg
     1561 atgtgcccct tttttattcc tgttcccccc gttttcacta taaatataac tccctcccat
     1621 tgcattaaaa ctgtaacgac tctcttggta acctgctatt agtccaagtc gataatccaa
     1681 attatttaat aaccaacctt taagattcag ctcgaattcg ttagcatcac ggagatgcgt
     1741 attgggatgc cagctctggt cggtccatac ctgaggggta ttggaattgt tccagtcttt
     1801 atcaaccatg cttgctttct ggttacctaa agtagtccaa ccggaaactc cgaaagacac
     1861 ttttggaatt agcttccaat cgatgccacc tctaacaata gtcgcattac tgtatttcca
     1921 gtccagttga ctaatttttc tccccccttc cttgggatga taaacgcgtt cttttgtttt
     1981 gccactcaga gatcctagac taatatccgt actgatgtta tccggtatga aaagtggata
     2041 gttagtggta gcatgtgtag taaagatcgc aggtacacaa agtgcaagta caaggaattt
     2101 taatttcatc gagaactcta cttttttggt tgaaatgtcc atgatcttct tactttataa
     2161 gataaacgtc aatgccaagc attattttaa ttaaatattt gtttatgtta ccaagaacat
     2221 atcttttatt ataatattta ttgaactttt gtaaaaataa aaaaatacct cattgtgcga
     2281 ataaagtaac gggggccata gatttctcta cctacctttt ggtgttcata cctgaggctg
     2341 atagtttcag gattccgcga caattctact caccttcttg ttctctcgcc aatatgaaat
     2401 caatcagaaa agcgttccat aagaacaacg ataagacaat aaattgtcat cacggctgca
     2461 atagccaacg tgcctttcag taccaacatg acaacctcct gtatttgacg taacgtgtgc
     2521 tgataaatat gtatttgtat ctcaactgac tcaatacaac acttaagtat agtttttcag
     2581 attttgtttt tcccgtgaaa tattatggaa atggcagaag caatacattg aattccgatt
     2641 cgagtgaata cggctagcgg gcacctcact ttagcactga agccatcaac ttttatttga
     2701 cttatttcaa tggaaagctt tctcactatc agtacaggta attcccatcc ttttaaagtc
     2761 gagagtcaga ctgcgactaa tacagttaaa gtgaccaaac tcagttatcc tctacacatt
     2821 ttacctcatg tttacttgga caattcattg gtgcttttat aaaagaataa atattctact
     2881 aatttaatgc atcacctaaa atagtatttt ttataataga actgtatcta tagtatatta
     2941 ttaaagctta attatttatt atgctttaat gtggttgtcc gattaaggac taatccttga
     3001 aaacgccgta tagggcggga gttcactcta ttgcgataac gtaaccgtac aatattgtgc
     3061 gtttcccaaa ttgaccacta ctgaactatt ctgggattta aatgcaataa aatggttggt
     3121 tgaaggtcgt ggtgatgatg ttagtccttt tgagaatatt ccagtgccag atcttactta
     3181 taataaagag gcttggcagt ttggataagc tattgtcctt attccactaa tacaaacttt
     3241 ttgtaaagta tattaaatat acttcatttc atactgaaat ccccggaagt attcaatcaa
     3301 acaattacac cataaaagga agagggagcc ccaattaagt tatcaagcat gtttatgata
     3361 cctgcagggt ataaattttc tggctgcaag cagacacata taaaacgtct ttattgatcc
     3421 tacccacata atatggacgc agtcgtaagt gaggctctgt gagcctaccc acgtaatatg
     3481 gacacaggcc taagcgaggt tctggttttc aaattgttcc ggactgaggc cgccacacca
     3541 acggtgccgt cgccagcgat tgtaatcgca ctcgatataa ttaaacactg ttgcccgcac
     3601 tatttcccga tagataaagt gttctccgtg gagacattcc acttaggaac aattatattg
     3661 ttcatggtaa tgtttatatt tgagtataga gattaaaatg attagtccca tcaagaatat
     3721 taagaacgta tttccaataa atactgcaaa tactgaatat atagtgagaa atatttatcc
     3781 tagagttgaa catggttact ttaatgaatc accaaacata tatgacaaga agtatatctc
     3841 tggtataacc agaagtatgg cacaattaaa aatagaggag tttattaatg agaaatccag
     3901 acgacttaat tatatgaaga caatgtattc tccatgtccg gaagattttc agcctatttc
     3961 cagagacgaa gccagtatac cggaaggaag ctggttgaca gttatatctg gaaaacgtcc
     4021 tatggggcaa ttttctgtgg atagtttgta ccatcctgac ttacatgcat tatgtgagct
     4081 cccagagatt agttgtaaga tcttccctaa agaaaatagt gattttttgt atataattgt
     4141 tgtattcaga aatgattctc cccaaggaga actacgggca aatcggttta tagaactata
     4201 tgatataaaa agagagatta tgcaagtgtt aagagatgag tcgccagagt taaagtctat
     4261 taaatctgag attattattg ctcgtgaaat gggagaactt tttagttatg catcagagga
     4321 aatagatagc tacataaaac aaatgaatga caggttttct caaatcaagg ctcgaatgtc
     4381 tgttacttaa tgtatattaa ttgt
//
LOCUS       DAJVUJ010000230         4390 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.232, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000230 DAJVUJ010000000
VERSION     DAJVUJ010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4390
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.232"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..358
                     /locus_tag="OQQ48_003975"
     CDS             <1..358
                     /locus_tag="OQQ48_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1954553.1"
                     /translation="WPGEGGEGHLSSFFVQTDKASINVAYHHTVNQQKELGTFGAFDH
                     DWHTLAFKFKGSNSINVTPVLDGVDGQAFDLVKWANTANGLNRFVITDITGSAETYPV
                     LIDTVEVKANKAGAAA"
     gene            498..680
                     /locus_tag="OQQ48_003976"
     CDS             498..680
                     /locus_tag="OQQ48_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HCS1954554.1"
                     /translation="MTFIHQVMLYFCTAVCVLYLLSGGYRAVRDFWCRQIDKRAAEKI
                     SASQSAGSKPEEPVTP"
     gene            604..951
                     /locus_tag="OQQ48_003977"
     CDS             604..951
                     /locus_tag="OQQ48_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HCS1954555.1"
                     /translation="MIKGPLRKSAPVSQPEANPKNPLLLNNPFQRENPMSEITSLVTA
                     EAVKEVLRSEEVRSALKQKLRQNLESRLDAEVDAILDELLGVAAVPEPEGIAGDGSAS
                     DGGEPTPDSDMMM"
     gene            1028..1243
                     /locus_tag="OQQ48_003978"
     CDS             1028..1243
                     /locus_tag="OQQ48_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCS1954556.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRFSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            1248..1592
                     /locus_tag="OQQ48_003979"
     CDS             1248..1592
                     /locus_tag="OQQ48_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HCS1954557.1"
                     /translation="MKKKYELVVKGINNYPNKITVTVALEIGGYPSLLLPDVAISLDR
                     TEDATLEFYEAEAKKQAKQFFMDVAAGLCEGDGPLPEKRPVILEAQDVLITYRGKLPG
                     IITGSLKTPPLA"
     gene            1643..2176
                     /locus_tag="OQQ48_003980"
     CDS             1643..2176
                     /locus_tag="OQQ48_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCS1954558.1"
                     /translation="MNTKIKYGLSAAVLALIAAGAPAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAILVDSKPVVPGMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYRRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWDIDR"
     gene            2332..2514
                     /locus_tag="OQQ48_003981"
     CDS             2332..2514
                     /locus_tag="OQQ48_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310767.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1954559.1"
                     /translation="MAKYKRILLRLTMKNGLELKAPVTDDISRALAFAIKWVAVGVAV
                     SPMLYGLAKLVIALKS"
     gene            2841..2993
                     /locus_tag="OQQ48_003982"
     CDS             2841..2993
                     /locus_tag="OQQ48_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001228697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HCS1954560.1"
                     /translation="MLPLVVSACGSTPPAPVPCVKPPAPPEWIMQPAPDWQTPLNGII
                     SPSESG"
     gene            3079..3303
                     /locus_tag="OQQ48_003983"
     CDS             3079..3303
                     /locus_tag="OQQ48_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1954561.1"
                     /translation="MKKLLVTVKPFQGTIPFRILQRGRVLVEGSFSGKCTQLHSRTFQ
                     VNATNEELTVECTMNAAKCRMVSAALQPVC"
     gene            3648..3974
                     /locus_tag="OQQ48_003984"
     CDS             3648..3974
                     /locus_tag="OQQ48_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HCS1954562.1"
                     /translation="MKRNLPLIILLSSLVMGCTQHKTDMPRQLVKALPQYPAYAAANY
                     IKGRVDVKFDIGADGTVTRIEFIRSEPHHLFDEQVVKAMAKWRFEKDRPCKGVKKTFI
                     FSPSAP"
     gene            complement(4106..4306)
                     /locus_tag="OQQ48_003985"
     CDS             complement(4106..4306)
                     /locus_tag="OQQ48_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HCS1954563.1"
                     /translation="MSSKNRTRRTTTRNIRFPNQMIEQINIALDLKGSGNFSAWVIEA
                     CRRRLATDATHLRPASMKNNEK"
BASE COUNT         1121 a          937 c         1208 g         1124 t
ORIGIN      
        1 atggccgggt gagggtggtg aaggccatct gagttctttc tttgtccaga cagataaagc
       61 cagtattaat gttgcatacc atcatacagt taatcaacaa aaagaacttg gcacgtttgg
      121 cgcattcgac catgactggc atacgcttgc atttaaattt aagggcagta acagcattaa
      181 tgttactccg gtgcttgatg gtgtggatgg acaggcgttt gacctggtga aatgggccaa
      241 tactgctaat ggactcaaca ggtttgtcat tacggatatt acaggtagtg cagaaaccta
      301 ccctgtactt attgatacgg tggaagttaa agcaaacaaa gctggagcag ccgcataatt
      361 gctaaaaaaa gccgccagcg gcaggaatgg aagctggcgg aggtaatccc aatggagaat
      421 gtaaagaaaa gatgctttcg tacattggtt ttttaaatga aaacaattct cattgtcaac
      481 cataacggta agaaattatg acatttattc atcaggtgat gctgtacttc tgtacggcgg
      541 tctgtgtgct gtatcttctt tcgggtgggt acagggctgt gcgcgatttc tggtgcaggc
      601 agattgataa aagggccgct gagaaaatca gcgccagtca gtcagccgga agcaaacccg
      661 aagaacccgt tactccttaa caaccccttt cagcgagaaa atcccatgtc agaaatcaca
      721 tccctggtca ctgcagaggc agtgaaggaa gtcctgcgct ctgaagaagt ccggagcgca
      781 ctgaaacaga aacttcgcca gaatcttgag tcgcgtcttg atgcagaagt ggatgccatt
      841 ctggatgagc tgctgggcgt agcagcggtt cctgagccgg aaggtatcgc gggtgacggg
      901 agtgcttcag atggcggtga acctacacct gacagcgaca tgatgatgta agcatgcgca
      961 agggaccatc ggtgtgtgcc ggtggtcttt atattgttgt gagcttccgg attgcgggag
     1021 acggggtatg taccagatgg aaaaaatcac aacaggtgtg tcatacacca cgtcagcggt
     1081 gggaacgggc tactggttcc tgcagttgct ggacaggttt tccccgtctc agtgggcggc
     1141 aataggcgtg ctggggagtc tgttgtttgg gctgctgaca tatctgacta acctgtattt
     1201 caaaatcaga gaggaccgtc gtaaggcggc gcggggagag taaagcgatg aagaaaaaat
     1261 acgaactggt tgttaaaggg ataaataatt acccgaataa gattactgtt actgtggcac
     1321 tggaaattgg tgggtatccg tcactgttgt tgccagatgt ggcgattagt cttgaccgta
     1381 ctgaagatgc cacgctggag ttttacgaag ctgaggcgaa aaagcaggcg aagcagtttt
     1441 tcatggatgt tgctgccggg ttatgtgaag gggatggtcc gttgccggaa aagcgccccg
     1501 taattttaga ggcgcaggat gtgttgataa cctacagagg aaaactaccg ggaataatta
     1561 ctggttctct gaagactcca ccgctggcct gaagacttaa catatccagg gatttgaaat
     1621 cgataaaccc tgataaatat ccatgaacac caaaatcaaa tacggcctgt cggctgccgt
     1681 tctggcgctg attgccgcag gtgcgcctgc gcctgaaatc ctcgaccagt ttctggatga
     1741 aaaggaaggt aaccacacca cggcataccg tgatggcgcg ggtatctgga ccatctgccg
     1801 cggtgccatc ctggtggata gtaaacctgt cgtcccgggc atgaagttgt cgaaggaaaa
     1861 atgcgaccag gtcaacgcca ttgagcgtga taaggcgctg gcatgggtgg agcgcaatat
     1921 taaagtgccg ctgaccgaac cccagaaagc gggtatcgcg tcattttgtc cctataacat
     1981 tggccccggt aagtgtttcc cgtcgacgtt ttacagacgg attaatgctg gtgaccgcag
     2041 gggagcatgc gaagcgattc gctggtggat taaggacggt ggcagagact gccgtatccg
     2101 ctcaaataat tgctacggtc aggtatcccg gcgagaccag gaaagtgcgc tggcgtgctg
     2161 ggacatcgac agataagcag aatattttgc tgaaaataag gcatgagcac gcggacggat
     2221 aacacgaaat cctgcgaact ggcgaaacgt aagtgaataa aagtaaaaac cccgtttgtt
     2281 ggcaccaggc ggggttttgt gtttctgacc ttgagtaagg caagggagaa catggcgaag
     2341 tataaacgaa ttctgttgag gttgaccatg aaaaacggcc ttgaactgaa agcgcctgta
     2401 actgatgaca tcagcagagc actggctttt gccattaagt gggtggcggt cggtgttgct
     2461 gtgtccccga tgctgtatgg gctggcaaaa ctggtcattg cgttgaaatc gtgaagggag
     2521 gattaagcat gtcagacaaa ctcataacgc tggcgaagat cctctgtgta attgtcggca
     2581 tttcattttc actaatgctg gttgctcttt ttctttccat ggcctggatg atgttgtctt
     2641 cgtcggggct gctggggtga acataaaccg aatgctttcc gcgtttaccg ttattctgct
     2701 ggtggtctgt ggtgcgctgt ggctggcaac agaccattac cgtgataacg ccatcaccta
     2761 caaagcgcag cgcgataaaa aagccagaga gctggagctg gcaaacgcaa ccattactga
     2821 catgcagcag cgccagcgtg atgttgccgc tggtcgtaag cgcctgcgga tcaacgccac
     2881 ctgctccggt accgtgcgtg aagccaccgg cacctccgga atggataatg caaccggccc
     2941 ccgactggca gacaccgctg aacgggatta tttcaccctc agagagcggt tgatgacaat
     3001 gcagaagcaa ctggaagggg cgcaggaata tatccgcact cagtgtatta actagtattt
     3061 ttgttatccg gagaatgcat gaagaaatta ctggtaaccg taaagccttt tcagggaaca
     3121 attccgttcc gtattttgca gcgtggtcgt gttcttgttg aaggttcgtt cagtggtaaa
     3181 tgtacgcaat tacactcccg gacctttcag gtgaatgcca cgaatgaaga gctaaccgtt
     3241 gagtgtacga tgaatgccgc taaatgccgc atggtatccg ctgcattaca gccagtgtgt
     3301 tgagcgacct tattaaccat gcgcggtatt gtcgccgtat ccccgcatta acagagaccg
     3361 cagcccgacc gggagactcc tctgcgcgag tgtgcgggga taatcaaaaa cgatacacac
     3421 cggggtttac cgcgttaacg gagcgcggcg ttgtcccctc atggtcgctg gtccggtgcg
     3481 atggtggaag aaaccggacg atgtgttacc tcgcaagctc tgttatgtca tgtgtctgat
     3541 ttgtgattta agtcggataa ttgtcgttgc cattaagcag aggattgatg accgacaggg
     3601 tggcattgtt agaataagac ttattcttat ctgtgccggg aatgaaaatg aaaagaaacc
     3661 ttccgttaat tattttgttg tcttctctgg ttatgggctg tacgcaacat aaaacagata
     3721 tgccccgaca gttggttaag gcattaccac aatatccggc ctatgcagcg gcaaattata
     3781 taaagggacg ggttgatgtg aagtttgata ttggtgctga tggtactgtc acccgaattg
     3841 agtttatccg ttcagagccg caccatttgt ttgatgagca ggttgtaaag gcgatggcaa
     3901 aatggcgatt tgagaaggac aggccgtgta aaggcgtgaa gaaaacgttt atctttagtc
     3961 cttctgcacc ctgattattt catcagaaat taattatcac tctgttgtta ttctgtacat
     4021 cccggcaggg taagtcttgt tccgtcggat atgaagatga aatattgttg gaggacagtg
     4081 ggtacctgct cctgtaaccg aacgttcatt tctcgttatt tttcatgctg gccgggcgca
     4141 gatgcgttgc atctgttgcc agccttctcc tgcaggcttc aataacccac gctgaaaagt
     4201 ttcctgaacc tttcagatca agagcgatgt taatttgttc aatcatctgg tttggaaatc
     4261 ggatgttgcg ggttgttgtt ctgcgggttc tgttctttga tgacataatg tttccccata
     4321 ttcagtgttg ctgatttgta ttatctgaag ttgcttttac gttaatttga tgcagatcaa
     4381 tcaatacgat
//
LOCUS       DAJVUJ010000231         4379 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.233, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000231 DAJVUJ010000000
VERSION     DAJVUJ010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4379
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.233"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(24..1238)
                     /gene="ydcO"
                     /locus_tag="OQQ48_003986"
     CDS             complement(24..1238)
                     /gene="ydcO"
                     /locus_tag="OQQ48_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HCS1954564.1"
                     /translation="MLRCLYAITYEVTMRLFSIPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVARATTAQISGWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRIIPHSLAAAMLAEILLRFGLQAFASLDGQ
                     FTLCGSMLLVWLATKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPD
                     FSFAHSLSVALPLFLVTVASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAVAGIFYLLAGLFGSAITEMMAALPVSWIQ
                     MLAGLALLSTIGGSLYQALHNERERDAAVVAFLVTASGLTLVGIGSAFWGLIAGGVCY
                     VVLNLIADRNQY"
     gene            1291..1827
                     /gene="sutR"
                     /locus_tag="OQQ48_003987"
     CDS             1291..1827
                     /gene="sutR"
                     /locus_tag="OQQ48_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HCS1954565.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQQQAMVITSLFPYDPQLCFEHF
                     SIQMASGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            1900..3861
                     /gene="rlhA"
                     /locus_tag="OQQ48_003988"
     CDS             1900..3861
                     /gene="rlhA"
                     /locus_tag="OQQ48_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HCS1954566.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLDQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMNYVKNITAHYRQMLDAIIEERGDLARAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLDV
                     AVTEPLANGDGLNVMIKREVIGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVAEPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(3953..4183)
                     /locus_tag="OQQ48_003989"
     CDS             complement(3953..4183)
                     /locus_tag="OQQ48_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HCS1954567.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
BASE COUNT         1135 a         1127 c         1088 g         1029 t
ORIGIN      
        1 cgtctttcag ggcgtgtagg atgtcaatat tggtttctgt cagcgattaa attcaacacc
       61 acgtaacaaa cgcctccggc aattaatccc cagaacgcag aaccaatccc gaccagcgtc
      121 aatccacttg ccgttaccag aaatgccacc accgccgcgt cgcgctcacg ctcattatgc
      181 agcgcctgat acaaactgcc gccaatggta ctcaacagcg ccagacctgc cagcatctgg
      241 atccaactta cgggcagggc agccatcatc tcggtaatgg cactaccaaa cagacctgcg
      301 agcaaataga aaatgcctgc aacggcagct gccagccaac gttgatcttt atccggatgc
      361 gcttccgggc tttggcaaat agccgcggtg attgccgcaa taccgacgga ataaacgccg
      421 aaaggggaaa aaaccagtgc cagcaatcca gtaaatacaa ttaatggcga aacaggagcc
      481 gaatatccag ctgctttcat tgctgcgata cccggtgcgt tttgcgatgc caccgtcacc
      541 agaaaaaggg ggagtgcaac gctcaggctg tgagcaaacg aaaaatcagg ggtaatataa
      601 gtggggagaa cgggtttaaa gacaacatca gttgtgacaa cgtcaccttg cgcgatgacg
      661 atcacgatcc caataatcat cgcggcaatt accgcatagc gcggcgcaac ggccttggtt
      721 gccagccata ccagcaacat acttccacac aacgtaaatt gaccgtccag actggcaaac
      781 gcctgtaaac caaagcgtaa taaaatctcg gcaagcattg ccgccgcaag cgagtgcgga
      841 ataatgcgca tcagacgagc aaagagtccc gttatgccgc agaggactat taacgcgttg
      901 gtgacaataa aaacgccgat ggcttcgtta agtgttagtc cctgcaatcc ggtgaccaac
      961 aaagccgcgc caggcgttga ccatgcggtg agaacaggta cgcgatacca taatgtcaga
     1021 gtcagcgtac tgacgcccat tgccagcccc agcgccgtca tccagccaga gatttgtgca
     1081 gtggtggctc tggcgacaat cgctgcttgc cagattattg ccgctgaact ggcgtagcca
     1141 attaataccg ccagaaaccc cgccagtagc gtgggtggag gaatagaaaa cagacgcata
     1201 gtcacctcgt acgttatagc gtacaggcac cgtagcattt gtccgttata acgcacaagt
     1261 gataaacttc cgttttgccg gaggagtcgc atggaaaatc tcgctcgctt tttatccacc
     1321 acacttaaac aactacgcca gcagcgcggc tggagtcttt cgcgactggc agaagcgacg
     1381 ggcgtttcta aagcaatgtt agggcagatt gagcgtaatg agtccagtcc gacggtcgcg
     1441 acattatgga aaattgccac cgggttgaac gtaccatttt caacatttat ttctccgccg
     1501 cagtccgcta cgccttcagt ttatgatcca caacagcagg cgatggtgat cacttcgctg
     1561 tttccttatg atccacaact ctgctttgaa cacttctcga ttcagatggc atctggtgca
     1621 ataagcgaat cgacgcccca tgagaagggg gtaatcgaac atgtcgtcgt cattgatgga
     1681 caacttgatc tgtgcgttga tggggaatgg cagaccctta attgtgggga aggcgttcga
     1741 tttgctgcag acgtcacgca tatctatcgt aatggtgggg agcaaaccgt acattttcat
     1801 tccctcatcc attacccgcg cagttaagcg ggaaaactat ttcgcaacgt cgcgcttgtg
     1861 gctaaaatag ccgccatttt tcagctactg gataagaatg tgaccgtatc ttctcatcga
     1921 cttgaactgt taagcccggc acgcgatgcc gccattgccc gcgaagctat tttgcacggt
     1981 gccgatgctg tttatatcgg cggccctggt tttggtgccc gtcataatgc cagtaatagc
     2041 ctgaaagata ttgccgagct ggtgccgttt gcccatcgtt atggtgcaaa aattttcgtc
     2101 acgcttaaca ccattttgca tgatgatgag ctggaacccg cgcaacggct gattactgac
     2161 ctctaccaga ccggtgtcga tgcgctgatt gttcaggata tggggattct ggaacttgat
     2221 attccgccga ttgaactgca cgccagtacg cagtgcgaca ttcgtacagt tgaaaaagcg
     2281 aagttcctct ctgatgttgg cttcacgcag attgtgctgg cgcgagagct gaatcttgat
     2341 cagatccgcg cgattcacca ggctacggac gcgaccattg aattctttat tcatggggca
     2401 ctgtgcgtgg cctattcggg tcagtgctac atttctcatg cgcaaacagg gcgtagcgcc
     2461 aaccgtggcg attgctcgca ggcgtgccgt ttgccataca cattgaaaga cgatcagggg
     2521 cgggtggttt cctatgaaaa acatctgctg tcgatgaaag ataacgatca gactgccaac
     2581 ctcggcgcgc tgattgatgc tggtgtacgc tccttcaaga ttgaaggtcg ttacaaagat
     2641 atgaactacg tgaagaatat caccgcccat tatcgccaga tgcttgatgc cattattgaa
     2701 gaacgtggcg atctggcgcg cgcttcatca ggtcgtactg aacatttctt tgttccatcg
     2761 acggaaaaga ctttccaccg tggtagcaca gattattttg tgaatgcccg taaaggcgat
     2821 attggcgcgt tcgattcgcc gaaatttatc ggcctgccgg tgggtgaagt attgaaagtg
     2881 gcgaaagatc atctcgatgt tgccgtcacc gagccactgg caaatggtga tggcctgaac
     2941 gtgatgatta aacgtgaagt catcggtttt cgtgccaata cggtcgagaa aaccggagaa
     3001 aatcagtacc gcgtctggcc caatgaaatg ccagcagatt tgcacaaaat tcgtccacat
     3061 cacccactaa accgtaatct tgatcataac tggcagcagg cactgacaaa aacctccagc
     3121 gaacgtcggg tggcggtaga cattgaactg ggcggctggc aggaacaact gattctgacc
     3181 ctcaccagtg aagagggtgt cagcatcacg catacgctgg acgggcagtt cgacgaagcc
     3241 aataacgccg aaaaagcaat gaacaatctg aaggatggtc tggcaaaact ggggcaaacc
     3301 ctctattacg cccgcgatgt gcaaattaat ttgccggggg cgctgtttgt accaaacagt
     3361 ctgttaaacc agttccgccg tgaagctgct gacatgctgg atgctgcgcg tcttgccagt
     3421 taccagcgcg gcagccgtaa accggttgct gagcctgcgc cggtttatcc gcaaacgcat
     3481 ctgagtttcc tcgcgaacgt atacaaccag aaagcgcgtg aattttatca tcgctatggt
     3541 gtgcagctga ttgacgcggc gtatgaggca catgaagaga agggcgaagt cccggtgatg
     3601 atcaccaagc attgtctgcg ctttgccttt aatctgtgcc cgaaacaggc gaaaggcaat
     3661 atcaaaagct ggaaggcgac gccaatgcaa ctggttaacg gcgatgaagt attaacgcta
     3721 aagtttgatt gccgcccatg cgagatgcac gtcattggca aaatcaaaaa tcacatactg
     3781 aaaatgccgt taccgggaag cgtagtggca tccgtaagtc cggatgagct gctgaaaaca
     3841 ttgcctaagc gaaaagggta aaacgccagt tttctggtta ctcacaactc attgaatctg
     3901 catgatattg cctgccgggt aaggcgtcac gccgcatccg gcatcaaacg actcaacgtt
     3961 gtcccgcttc tggtttgcgc gatttttgcc agtaaaaatg ctcgcgcaaa ccttccgccg
     4021 actcttccgc cacagcacgc aattcatcgc tgtccgcttc atgacgcagc tgatgatcca
     4081 cattctttac ccacacaaat tcatgtcctt tgtgccctgc catgagttgt cctgagaaca
     4141 gcgcacacgt taataagaca accgataacg ccttcgtaaa catcctgcca cctttttctt
     4201 accttttgcc gctatgatgc cgatcgtttc ttgaggttat tattcagttt tgcaaattag
     4261 cgcaaagaaa ttctggaatc ttccttcctg attttgcatt gcattctgtc gttgcggcga
     4321 tttagtgcta ttttcgagca aattacacac agaggtaaac actaatgttt actttgttg
//
LOCUS       DAJVUJ010000232         4319 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.234, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000232 DAJVUJ010000000
VERSION     DAJVUJ010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4319
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.234"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(<1..975)
                     /locus_tag="OQQ48_003990"
     CDS             complement(<1..975)
                     /locus_tag="OQQ48_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 family protein"
                     /protein_id="HCS1954568.1"
                     /translation="MIRKSATGVIVALAVIWGGGIWYTGTQIQPGVEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSHFQMQISFDNGAPDLNIKPGQKVAFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLTRLE
                     EKKFPVGESESKFNQVNIINQGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEV"
     gene            complement(1130..1756)
                     /gene="dsbA"
                     /locus_tag="OQQ48_003991"
     CDS             complement(1130..1756)
                     /gene="dsbA"
                     /locus_tag="OQQ48_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HCS1954569.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
     gene            complement(1773..2759)
                     /gene="srkA"
                     /locus_tag="OQQ48_003992"
     CDS             complement(1773..2759)
                     /gene="srkA"
                     /locus_tag="OQQ48_003992"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HCS1954570.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRIVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDSTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(2836..3105)
                     /locus_tag="OQQ48_003993"
     CDS             complement(2836..3105)
                     /locus_tag="OQQ48_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HCS1954571.1"
                     /translation="MKCKRLNEVIEFLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            3175..3759
                     /gene="mobA"
                     /locus_tag="OQQ48_003994"
     CDS             3175..3759
                     /gene="mobA"
                     /locus_tag="OQQ48_003994"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HCS1954572.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPPDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            3741..4268
                     /gene="mobB"
                     /locus_tag="OQQ48_003995"
     CDS             3741..4268
                     /gene="mobB"
                     /locus_tag="OQQ48_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HCS1954573.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGARHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT         1169 a         1020 c          966 g         1164 t
ORIGIN      
        1 aacttcatcg tttgctaact cagggtgtgc agcaagctgc ttctgcatcg caatgttata
       61 ctggatgata aattggcgca ctgctgaggg atcaatagat tcagcaatca atgaccactc
      121 accactaccg agttgctgat tcccttttgt taacttatca agttcgtagg tcagattgac
      181 attgatataa tctttgtctt taacgcgatc cagcctggtt tttgcaacga aagcatcgat
      241 ttgggcaacg tcttccccct gattgataat attaacctga ttaaatttcg actcgctttc
      301 accaaccgga aatttctttt cttctaatcg tgtcagagaa tcaatggtaa aactttttgc
      361 cgttactttg ttcattggtg ataattgcag aacaatatct tcgactttgc cttcaatatc
      421 caggttagac aatgaactac catcaccatt aaaagtaaac tggccctcac caaaagaaaa
      481 ctttccatac tctgcagggg caacatctaa tgtcgtagag aatgagccac caaacgcgaa
      541 tcgcaatgtc gcttccacgg gcgatttatt tttcgcggcg ataaatagcg gttgtgtcag
      601 ttcattattc actaagttca cttttgccgc tgccagtgct gggatgacat taccatgcat
      661 taacattgtg atgggcaacg gaccgtgctc aacatccaca tcaaatgcaa ctttctggcc
      721 tggcttgata ttgagatcgg gtgcaccgtt atcaaatgaa atttgcattt gaaaatggga
      781 attaaaaaag cctttgtcaa agtttttata acttaacgtc atatcgtagg catgttcacc
      841 tttcttttta gcatcgttaa aatctttaat aaatttttcg acaccaggct gaatttgcgt
      901 acctgtgtac catatgccac caccccagat tacggctaac gcaacaataa cacctgtagc
      961 tgacttacgt atcatataaa tattccctga taaaacagca aaactttgaa tatccactta
     1021 tgctgaaata atgtccagcg gcaggatgta ttatcagaat gtatttatat ttattaaata
     1081 ataaaaaaag cccgtgaata ttcacgggct ttatgtaatt tacattgaat tattttttct
     1141 cggacagata tttcactgta tcagcatact gctgaacaaa aacatccata ttgctggtat
     1201 ccataccctg cggattcagc tgatatttac cgttaacaaa catcgccgga acaccgcgca
     1261 attgcacgtc agctgcagct ttttcctgct gagctaccag agatttcacc acgaagctgt
     1321 tccacgccgc gtcgtactct tcacctttaa tacctgcgtt gataaataca tcgcggatat
     1381 cagaagcaga acgaatggtc tgggttttct gtacgccttc aaacagcgga acagtcactt
     1441 tgtcttccac gcccagcgcc atcgccacag cccatgcctg agtcagatct ttgcccaggt
     1501 caccacccat gaagttgacg tggtatttag tcatcttcac gccttccggc agttttttct
     1561 tcacattatc agaaatatgc agaacttctt caaactgata gcagtgcgga cagaagaaag
     1621 agaaaaactc cagcacttgc ggcgcgccag ctaccggttt ttccagggta gtgtactgtt
     1681 taccatcttc atactgcgcc gccgatgcgc taaacgctaa aactaaacca gccagcgcca
     1741 gccaaatctt tttcatgatc tactctctcc gattaataca taggtgttaa ttgtaaaggg
     1801 ggttcttgta gaacttttgc ctgttctata aaagtcgccg tttgtcgcag ccagtaatct
     1861 tccccggtta accacgggaa atttttcggg aacgcgggat cagcccaacg ccgcattagc
     1921 caggcaagat aataaaccaa acgcatggcg cgtaaaggtt caatcagtcc gatttcagcg
     1981 gtgtcgaact cgctaaattc ttcataagct tcaataatag tttccagttg catccgctgc
     2041 tcggctttat cgccattgag caacatccac aaatcctgaa tagctggacc attacgtgca
     2101 tcatccagat caacaaacat tggaccatcg cgccagagaa tattcccggc gtggcagtct
     2161 ccatgtaagc gcagaacggt ggaatcttcc cgccagtgcg ctgtaacggc ggcgatcagc
     2221 tcatctgtcg ctttcaggaa tgccgctttc aacccggaag gtatcagtgt agcgtcctca
     2281 aacagcttgc gtggctcaat gagatattca ttcagaccga tggtcgggcg atggataaaa
     2341 agctgtttgc gccccgtctg gtgcatacgc cctaaataac gcccaaccgc ttccatctga
     2401 tcgatattat cagcttcgaa ctggcgacca ccgacgcttg gaaaaacagc gaaataaaat
     2461 ccctgatgat tcaataaagt ctgaccgtta aaggccacag gtgctgcgac cggaacttca
     2521 tcatttacca actgcaacgc aaattgatgt tcttcgagga tttgatcggc tgtccaacgt
     2581 tcagggcgat aaaatttgac gacaatacgt cgacgatctt cgtcctgaaa ttgatagaca
     2641 cggttttcat agctgttaag cggggtaaga ccggaatcca cccggatccc ttgctcaaat
     2701 agagcatcca tgatggtatc cgggtgtagt gtctggaaag taaaagcgct gttgttcatc
     2761 ccatcatccg gaaaacatta cgaatgatga aggatatcat ttcacgatga ttttggtggc
     2821 gccgcttaca agcttttact ctttaattac gccgcgagcg cgtaacagcg ccgttttgaa
     2881 gtcttcttca taatctttct gcaaacccgg gatcaccgca tctttcgcgg aatcacgcat
     2941 tttcaggtga tagatcagaa tgtcatccgt caaatccgcc agttcgccgt caaaacctga
     3001 ctctttcgcc agtttctgca aaaattgcaa caggttaagg tctggctctt tttgccaggc
     3061 tggctggagg aattcaataa cttcattcag acgtttacat ttcatagtga tgctccttac
     3121 tcttgagaca gacacgttag cagggtcaat cccacaataa aagaggcgat atcggtgaat
     3181 ctgatgacga cgataacagg cgttgtgctg gcaggcggta aagccagacg aatgggtggc
     3241 gtagataaag gattgcttga attaaacggc aaaccattat ggcaacatgt cgctgacgcg
     3301 cttatgacgc agctctctca cgtcgtggtt aatgccaatc gtcatcagga aatctatcag
     3361 gcaagcggtc tgaaagtgat tgaagattca ctggcggatt acccaggccc tctggcagga
     3421 atgctttcag taatgcagca ggaagctggt gagtggtttt tgttttgccc gtgcgatacg
     3481 ccttacattc cccctgattt agcagcccgg cttaatcatc agcgcaaaga tgcgcctgtc
     3541 gtgtgggtcc atgacggtga acgcgatcac ccgactattg ctctggtaaa ccgcgctatt
     3601 gagcctttat tactggaata tctgcaagca ggagaacgcc gggtaatggc atttatgcgt
     3661 ctggctggcg gtcatgcggt tgatttcagc gatcataaag atgcatttgt taacgtgaat
     3721 acgccagagg agcttgcccg atggcaggaa aaacgatgat accgttactc gcctttgccg
     3781 cgtggagtgg taccggaaaa actacgcttt tgaaaaaact gatcccggca ttatgcgcca
     3841 gagggatccg tccagggctg attaagcata cgcaccatga tatggatgtt gataagccag
     3901 gcaaagatag ctatgagctg cgcaaggctg gcgcggcaca aaccatcgtt gccagccagc
     3961 aacgatgggc cttgatgacg gaaacaccag acgaagaaga gctggatcta cagtttctcg
     4021 caagtcgaat ggatacctca aagctggatt tgattctggt cgaagggttt aagcatgaag
     4081 agatcgcaaa gattgtgctg tttcgcgatg gagccagaca tcgacctgaa gaattagtga
     4141 tagacaggca tgttattgct gtagccagtg atgtgccgct taatcttgat gtcgcattac
     4201 tggatattaa tgatgttgag gggctggctg attttgtggt ggagtggatg caaaagcaga
     4261 acggataagc aagagtaggc tggatcagac gcagccagtg tcgcatccga cctttttct
//
LOCUS       DAJVUJ010000233         4301 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.235, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000233 DAJVUJ010000000
VERSION     DAJVUJ010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4301
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.235"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..465
                     /locus_tag="OQQ48_003996"
     CDS             <1..465
                     /locus_tag="OQQ48_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014837283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1954574.1"
                     /translation="LPISAADKRRFYYYFQEKNTPNIERFFVFDSSEYRYALNMREVV
                     FHQFLSDGLRPIVDEDDDAYEDDYFNVHITLVNGGPVIPLSVEPDAPQNEETDDIGQL
                     NAFFDALDCEPETTDRFMITDEDGEDAFIRIGSIAMVRVALDVLEPVEDDDE"
     gene            712..1977
                     /locus_tag="OQQ48_003997"
     CDS             712..1977
                     /locus_tag="OQQ48_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001642584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCS1954575.1"
                     /translation="MALTDIKVRAAKPTDKQYKLTDGGGMHLLVHPNGSKYWRLQYRY
                     EGKQKMLALGVYPEITLADARVRRDEARNLLANGVDPGDKKKNDKVEQSKARTFKEVA
                     IEWHGTNKKWSEDHAHRVLKSLEDNLFAALGERNIAELKTRDLLAPIKAVEMSGRLEV
                     AARLQQRTTAIMRYAVQSGLIDYNPAQEMAGAVASCNRQHRPALELKRIPELLTKIDS
                     YTGRPLTRWAIELTLLIFIRSSELRFARWSEIDFEASIWTIPPEREPIPGVKHSHRGS
                     KMRTTHLVPLSTQALAILKQIKQFYGAHDLIFIGDHDSHKPMSENTVNSALRVMGYDT
                     KVEVCGHGFRTMACSSLVESGLWSRDAVERQMSHMERNSVRAAYIHKAEHLEERRLML
                     QWWADFLDVNRERFISPFEYAKINNPLKQ"
     gene            complement(2294..2817)
                     /locus_tag="OQQ48_003998"
                     /pseudo
     CDS             complement(2294..2817)
                     /locus_tag="OQQ48_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535067.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            3404..3633
                     /locus_tag="OQQ48_003999"
                     /pseudo
     CDS             3404..3633
                     /locus_tag="OQQ48_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
BASE COUNT         1147 a          948 c         1047 g         1159 t
ORIGIN      
        1 ttgcccattt cagcagcgga taagcggagg ttttattatt attttcagga aaaaaacacc
       61 ccaaacatag aacgcttctt cgtttttgat tcttccgagt atcgttatgc gttaaatatg
      121 cgtgaagtgg tatttcatca gttcctttct gatggtctgc ggccaatcgt tgacgaagac
      181 gatgatgcct acgaagacga ttattttaat gtccatatca cgctggtaaa cggcggacca
      241 gtgattccac tgagtgttga gccagatgcg ccgcaaaacg aagagacgga tgatatcggc
      301 cagttgaatg cattttttga cgcgctggat tgcgagcctg aaaccacgga tcgttttatg
      361 ataaccgacg aagatggaga agatgctttc attcgtattg gttctatcgc tatggtgcga
      421 gttgcgcttg atgtgctgga accagtggaa gacgatgatg agtagtattg aatggattga
      481 tttcatattg ttgagtctgg aatcgcggtg cggccttcgg caccaaaagt atgtaaatag
      541 acctcaactg aggtcttttt ttatgcctga aatccagtgt ttatctatct ttcccgctat
      601 attaactctc tcaaggtcaa ccgatatcaa cgtacatcta ccaacatatg ttggtacaga
      661 tgatggtatt tccggttcga taatgcttgt accaacaggg agggaatacg catggcatta
      721 acagatatca aagtcagagc agccaagcca acggataagc aatataagct gactgatggt
      781 ggcggtatgc atctgcttgt ccatccaaat ggttctaagt actggcgttt gcagtaccgt
      841 tatgagggaa agcaaaaaat gctggcactt ggggtttatc ctgaaatcac actagcggat
      901 gccagagtac gtcgtgacga ggcgcgtaac ctgcttgcga atggcgtcga tccgggagac
      961 aaaaagaaaa atgataaggt tgaacagagt aaagcacgaa cctttaaaga agtcgcgatt
     1021 gagtggcatg gcaccaataa aaagtggtct gaagatcacg cccatcgtgt gctaaaaagt
     1081 cttgaagata atctttttgc agcgcttggt gaacgtaata tcgctgagtt aaaaactcga
     1141 gatttattag cacctattaa ggccgtagaa atgtctggac gtcttgaagt ggccgctcgt
     1201 cttcagcagc gcactacagc catcatgcgc tatgcagtgc aaagtgggtt aattgattat
     1261 aacccggcac aagagatggc tggggcggtt gcttcctgta atcgacaaca tcgtcccgcg
     1321 cttgaattaa agcgcatccc tgagttgctt acaaaaatag atagctatac tggtaggccg
     1381 ctaacccgat gggcgataga actcactttg ctgatcttta ttcggtccag tgagctgcgt
     1441 tttgctcgtt ggtcagagat cgatttcgaa gcgtctatat ggactatccc accggagcgg
     1501 gagcctattc ctggagtgaa acattcccat agaggctcaa aaatgcgtac aactcatcta
     1561 gtgcctcttt caacgcaagc tcttgcaatt ttaaagcaga taaaacagtt ttatggggcc
     1621 catgacttga tatttattgg tgatcacgat tcgcacaaac ccatgagtga gaatacggta
     1681 aatagtgcgt tacgggtcat ggggtatgat acaaaagtag aggtttgtgg tcatggcttt
     1741 cgaacaatgg cctgtagttc attggtcgaa tcaggtttgt ggtctcgtga tgctgttgaa
     1801 cgtcagatga gccacatgga gcgaaattca gtgagggccg cgtatatcca taaagcagag
     1861 catctggaag aacggcgatt gatgctacag tggtgggccg attttctgga tgtaaacaga
     1921 gaaagattta tcagtccatt tgaatatgca aagattaata atccattaaa acagtaatca
     1981 tcccgggcaa atgcccggga attattctag gattattttc tttgttaaaa aagacaaacg
     2041 gtattaactg atgtatttac tatttaccgc tccctgcagg ggggattatc agaatccgcg
     2101 gttattcatt atttatatac tttcaataaa aaagtaacct cataaaaaat cctgtactca
     2161 cagggtgatg cgggataact gacgtaagga gtgctgcagg gatttgatgg tcccttgtgc
     2221 tgatatgaat acggatattt tatacagaat acttttcagt acaagcgact gtattacagt
     2281 ctgccatata tgattacctg tgatcaggaa gaataagtgg ctgaggtttc aaactgtctg
     2341 ccggtatatt cctctctccc ttaaaaacca taacagttta attatcttcg tctgtcagcg
     2401 gattgaatgg cggtatattt tcagtacaaa taccggtcat ccactgaaaa atacctgcga
     2461 aatgacgggc actgattttt ctactgacgg gctgatgaga cgttatgtca ctgacatata
     2521 atccctgcgc acactctcac cagtgacaat aacaatcgta tcatataacg gtgctcccgg
     2581 ctggattttc cagttgtcag ccccgtgctg actcagttgt tcataacgct gcatttcacg
     2641 taatgtgtca gttgtcccca caactgttcc tttaaccatc cgcaatggcc aacggtttac
     2701 agagcataat acgaatagcc gtagagccag ccagttacgg tggtcgcgat ggtgtgtccg
     2761 ccagaaaatc accacgaata ccagaatcgc ggcactgacc agaaaatgat aaacaggaaa
     2821 cacggggcgg ttcactttgc tttctcctgt agtgttttct gtaggttctg aaccggttcg
     2881 agcggggttg tgaggctgat ggcaatcccc gggctcttcc tgcatgttac actggtactg
     2941 caagaggggg ccttcgctcc tctgacatta ctcagtattt tcactactac accccatctg
     3001 ccatccataa caaccgattc accccctcgc gaggcgttgt tgcccgtcac ccggacagac
     3061 atcgttatgg atttctcttg ttgcacgatc gatcaattgt tatgcatgct gacaccacaa
     3121 ccccggtggg tatgaccgat gcctttctca ctcttttccc ggtcatcgtc ggccttcccc
     3181 gttattgtgg agtgtcagca ccctcgacgg catttcgatg gctgctcagt gttcactcac
     3241 gttacggccc gcataacccg ttgacttcct gaagaagtcg tttcagggag agattcagcc
     3301 cttttgttac ctcctggacc gcttcttgtc cttccagtcg gagcgagagt tagccggggc
     3361 ggactttcac cgacggaaag atcgtgcatt tcaaggcaca cacaacaata tctgcgagcg
     3421 tgctatccgt ccggtcgtta tgggacgaaa ggcctggttg ttcgctggtt cactgatggc
     3481 cgggaaccgc gcggcacaga taatgagcct tctgggaacc gcagtctgga gccacatgct
     3541 tggctgacgg acgtcctgac gcgtctgccg gagtggccgg aggagaggtt ggctgagtta
     3601 ctgcctcttg agggctttac cttcttcggg taagtgatac ctgccgtcag gtgtttgtgc
     3661 accgggccat aacctgcagt tgggaattga acgcctgaca acaggaaatg agctagagca
     3721 gtaaacacgc gggctgacaa tgggtcagaa tatgagtcag cccggcgtta ttcctggggt
     3781 cagtacttca gatccagcct ccggtaccag actagctagc ccctacgcaa ccaaataaat
     3841 caaccatcgt cagagtgcaa tcgttcatat acactgtcgg tgatgcgctg aaaaatgcgt
     3901 taatgttgag ttcctcgaac ccttaccaat taagggacat agccagatac gcaactggct
     3961 gatgcaagca gtctaatgtc tttagatccg ctggcctgcg ggcatgacaa tgtcaaacag
     4021 tcaaagattg tggcgttgac ggtcagtctt ggggacaaaa aagctcagta ttctcgttgg
     4081 cagccagata atttaacagt tgatgctgaa acgaaatagg catcttggtt ctggtgtttg
     4141 taacaaacta tcagctacaa aaatatgctc aatttgtgac atcagtaaca aaacgcgttt
     4201 tgttatgtgg attgcttgtt ttttgatctg gtataacagg tataaaggta tacagaaaag
     4261 caaagaaata ctgcaaagga aaacagctat aacgtaagct a
//
LOCUS       DAJVUJ010000234         4295 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.236, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000234 DAJVUJ010000000
VERSION     DAJVUJ010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4295
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.236"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            47..2209
                     /gene="ybiO"
                     /locus_tag="OQQ48_004000"
     CDS             47..2209
                     /gene="ybiO"
                     /locus_tag="OQQ48_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HCS1954576.1"
                     /translation="MQWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGLWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIHDETARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLVGVRAPVQTY"
     gene            complement(2269..3195)
                     /gene="rlmF"
                     /locus_tag="OQQ48_004001"
     CDS             complement(2269..3195)
                     /gene="rlmF"
                     /locus_tag="OQQ48_004001"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HCS1954577.1"
                     /translation="MSAQKLGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            3471..3730
                     /locus_tag="OQQ48_004002"
                     /pseudo
     CDS             3471..3730
                     /locus_tag="OQQ48_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
     gene            3995..>4295
                     /locus_tag="OQQ48_004003"
     CDS             3995..>4295
                     /locus_tag="OQQ48_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCS1954578.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPG"
BASE COUNT         1000 a         1128 c         1075 g         1092 t
ORIGIN      
        1 agcatctata cttatctttt tgctctgttt tctcactgga ggagtcatgc agtggatcct
       61 gttcatcctc ttctgcctgc tgggcgcacc tgcccacgcg gtatccatac ccggcgttac
      121 aaccacaacg acaacggact caacgactga accggccccg gaaccggata tcgaacaaaa
      181 aaaagcggcc tatggcgcac tggcggatgt gctggataac gacacctcgc gtaaagaatt
      241 gatcgaccag ttgcgtactg tcgccgcgac gccccctgcg gaaccggtcc cgaagatcgt
      301 gccgccgacg ctggttgaag agcaaaccgt gctgcaaaag gtcaccgaag tcagccgcca
      361 ttatggtgaa gccctttccg cccgcttcgg gcaactttat cgcaatatta ccggctcccc
      421 gcataagccg tttaatccac aaacattcag caatgcgctg acccattttt caatgttagc
      481 ggtattagtg tttggttttt actggctgat tcgcctgtgc gcactgccgc tgtatcgcaa
      541 aatgggcctg tgggcgcggc aaaaaaatcg tgagcgcagt aactggttgc agcttccggc
      601 gatgattatc ggggcgttta ttatcgacct actgttactg gcactgacat tatttgtcgg
      661 ccaggtatta agcgacaacc tgaatgcagg cagtcgcacc atcgctttcc aacaaagttt
      721 gtttctcaac gcctttgccc tgattgaatt tttcaaagct gtactacgcc tgattttttg
      781 cccaaacgtg gctgaactgc gcccgttcac gattcatgac gagaccgccc gttactggag
      841 tcgtcgcctg agctggttaa gcagtttgat aggttatggc ctgattgtgg ccgtgccgat
      901 tatctctaat caggtgaatg tacagatagg tgcgctggcg aacgtcatca ttatgctgtg
      961 catgaccgtc tgggcgttgt acctgatctt tcgcaataaa aaagagatta cccaacattt
     1021 gctcaacttt gcggagcatt cgctggcctt tttcagcctg tttatccgcg cctttgcgct
     1081 ggtgtggcac tggctggcaa gcgcctattt tatcgtgctg tttttctttt cgttgttcga
     1141 tccgggcaac agcctgaaat ttatgatggg tgcaacggtg cgcagcctgg cgattattgg
     1201 tatcgcagcg tttgtttccg gtatgttttc ccgctggctg gcgaaaacca tcaccctctc
     1261 gccacatact cagcgtaact atccggagtt gcaaaaacgg ctgaatggct ggctttcggc
     1321 ggcgctgaaa acggcgcgta ttctgaccgt ctgcgtggcg gtaatgttgc tgttgagcgc
     1381 atggggattg ttcgatttct ggaactggct gcaaaacggc gcggggcaga aaaccgtaga
     1441 tatcctgatc cgtatcgcac ttattctttt tttctcggcg gttggctgga cggtgctcgc
     1501 cagtttgatc gaaaaccggc tggcttcgga tattcatggc cgcccgctac ccagcgcccg
     1561 tacgcgtacc ctgctgacgc tgtttcgtaa cgcgctggcg gtgattatca gtaccatcac
     1621 catcatgatt gtgttgtcgg aaatcggcgt caatatcgcg ccattgctgg caggtgccgg
     1681 ggcattaggt ctggctatct cgtttggttc gcaaacgctg gtgaaagata tcattaccgg
     1741 ggtgtttatt cagtttgaaa acggcatgaa cactggagat ttggtgacta tcgggccgtt
     1801 gaccggcaca gtggaacgga tgtcgattcg ctccgtgggc gtgcgccagg ataccggggc
     1861 gtatcacatc attccgtggt cttcgataac cacctttgct aacttcgtcc gcggtattgg
     1921 ttcggtagtg gcaaactacg atgttgatcg ccatgaagat gctgataaag ccaatcaggc
     1981 actgaaagat gcggtagcgg aattgatgga aaacgaagaa attcgcgggc tgattattgg
     2041 tgaaccgaat tttgccggga ttgtcggctt aagcaatacc gcgtttacac tgcgtgtttc
     2101 gttcaccacg ctgccactca aacagtggac ggtacgcttt gccctcgaca gccaggtgaa
     2161 aaaacatttc gacctggtgg gcgttcgcgc gccagtgcag acttattagg tgctgcctac
     2221 tccgggcgcg accccggctg aaccgttacc gccgggggag ccaacgcttt aacgctggcg
     2281 attgacaaaa cggcggcgct gctcgtcgtt cataaaggtc caggcaataa agcgactctg
     2341 cttttgcccc tgggccatct ctttttttac caccttcacc gcgcccacgt cggtcagagc
     2401 acgatacaac ggcggtaagt tttcaccacg agataccagc gaggtaaacc acatcacctg
     2461 cttcgcgaag cctttgctct cttcaatcat ctttttgata aaggcgactt caccaccttc
     2521 acaccacaac tcctgttgct gaccaccaaa gttcagtgca tcgtctttgt tcagccccag
     2581 gttacggcgt ttacgctcac tacctgcccg cgctgcagcg gcggaatcgt ggaatggcgg
     2641 gttacacaag gtcgcgtcgt attgctcgtt tttatggatg atgccgttaa aaattgcccc
     2701 actctctttt tgccgacgca gacgaatggc gcggttaagc cccggattag cactgataat
     2761 cgcctgcgca ctgcttaacg cctggctgct ggtttcacta ccggtaaaac gccagccata
     2821 ttcatgtacg ccaatcagcg gataaataca gttcgcacca acgccgatat ccagaatgct
     2881 ggcattcgct ggaattgttc cgcttgcttc agccagtaaa tcggcaaggt gatgaatata
     2941 atccgcccgg ccgggtactg gtgggcagag aaaaccgtcg gggatatccc agttcgctac
     3001 ggcgtaaaaa tgggccagca acgccttatt gagcgccttc accgccagcg gattggcaaa
     3061 gtctacgctt tgctccccgg cgggtgtaag cgtgaggaat tgcctgagtt caggattgac
     3121 ctgacaaagc gtggcgagat cgtagcggct gtgatgacgg ttgcgcggat gcaaccccag
     3181 tttctgggcg gacatagcat tctcctttaa gcagcggcgt aagatacccg cataacgcga
     3241 cagggtaaat atctggctgc gtttatcatt ccccaaaacc ttaacatggc tatggtttat
     3301 ggcaattcat cataaaaatc agcattatgc atgtcattga tttgtcttat tttgtaaaaa
     3361 tatatcgttc aaaaaataag cacatctctg tcaatttttt gcgtcatttg catcgcgcag
     3421 gcgacctaca cttatcaggc actacccgtt gtatttatga ggatgtcatt atgaaaaagt
     3481 gcctcacact actgattgcc accgtcctga gcagaatctc tctcacggct tatgccgcgc
     3541 aaccgatgag taacctggac agcggtcagc tacggcccgc ggtaccgttt cggcaactgg
     3601 cgcatcaaac ctaagcgatc tggaggataa actggcagaa aaagcgcgcg aacaaggcgc
     3661 gaaaggttat gtcattaatt ccgcaggcgg aaatgaccag atgttcggta ctgcaaccat
     3721 ctacaaataa ccccaccaac gtaactgcat tcgcctctgg atgcagtttt cgcctgtttt
     3781 gtaatcaaaa aacatcaaat atgaactcaa tgtaaataaa tgtatttctt tttcgcgcaa
     3841 tgggtgatag aaaatcgcgc taagtgataa tgtttatcaa aattattatc acttgcatga
     3901 acactatcac gggattaaca gtggcatcgc atccgcagag atgctttctc gtgacagtga
     3961 aaatttcaac atataagaaa aagtcacctg caaaatggaa aacaatcgca atttccctgc
     4021 cagacaattt cattcgctca cgttctttgc cggtctttgt attggcatca cgcctgtggc
     4081 tcaggcactc gccgccgaag ggcaaactaa cgcggatgac acgctggttg tcgaagcatc
     4141 aacgccttcg ctttatgcgc cacaacaatc tgccgatccg aaattctcgc gtccggtagc
     4201 ggatactacc cgcacgatga cggtgatttc tgaacaagtg attaaagatc agggcgcaac
     4261 caaccttacc gacgcgctca aaaacgtccc cggcg
//
LOCUS       DAJVUJ010000235         4267 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.237, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000235 DAJVUJ010000000
VERSION     DAJVUJ010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4267
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.237"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(<1..212)
                     /locus_tag="OQQ48_004004"
     CDS             complement(<1..212)
                     /locus_tag="OQQ48_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HCS1954579.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPV"
     gene            522..2294
                     /gene="aspS"
                     /locus_tag="OQQ48_004005"
     CDS             522..2294
                     /gene="aspS"
                     /locus_tag="OQQ48_004005"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCS1954580.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            2412..2864
                     /gene="nudB"
                     /locus_tag="OQQ48_004006"
     CDS             2412..2864
                     /gene="nudB"
                     /locus_tag="OQQ48_004006"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HCS1954581.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDSQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            2893..3633
                     /locus_tag="OQQ48_004007"
     CDS             2893..3633
                     /locus_tag="OQQ48_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCS1954582.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVITFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEIAATL"
     gene            3668..4189
                     /gene="ruvC"
                     /locus_tag="OQQ48_004008"
     CDS             3668..4189
                     /gene="ruvC"
                     /locus_tag="OQQ48_004008"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HCS1954583.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
BASE COUNT         1053 a         1061 c         1199 g          954 t
ORIGIN      
        1 tcaaccggct gttttaatgc ttcggcgtaa tcggcagacc agccaactcg caccagaaac
       61 acgggctgac cgctggcgcg aaatttcgcc gccagcttcc cggcgcgatt aactacctca
      121 tcggcagtat gtggaccacc ggcaaaaggt aagatgcctt cttgtaaatc gatcaccacc
      181 aacgcagtgg ttttagcatt aagttcaagc atgctgactc ctgaaaaaac aaaatcgtgc
      241 ctcacacctt aatgcgatta gccgatggcg gaacgacaaa ttttgttaat ttttatgagt
      301 tcgggcaaca cctttatagc aaactggcgt agagtctgat ttcaggcccg atataaagtg
      361 gtaacgaggc gttccagcgc gggctaggct cttacggtgc ggcagaattt ccagtataat
      421 agccgccttt tttcattcag ttgtgacata cagctaacgc tgcgacttgg tcacctgcgc
      481 agcagggggc atccgccggc agctaagtta agggatatct catgcgtaca gaatattgtg
      541 gacagctccg tttgtcccac gtggggcagc aggtgactct gtgtggttgg gtcaaccgtc
      601 gtcgtgatct tggtagcctg atcttcatcg atatgcgcga ccgcgaaggt atcgtgcagg
      661 tatttttcga tccggatcgt gcggacgcgt taaagctggc ctctgaactg cgtaatgagt
      721 tctgcattca ggtcacgggc accgtacgtg cgcgtgacga aaaaaatatt aaccgcgata
      781 tggcgaccgg cgaaatcgaa gtgctggcgt cctcgctgac tatcatcaac cgcgcagatg
      841 ttctgccgct tgactctaac cacgtcaaca ccgaagaagc gcgtctgaaa taccgctacc
      901 tcgacctgcg tcgtccggaa atggctcagc gcctgaaaac ccgcgctaaa atcaccagcc
      961 tggtgcgccg ttttatggat gaccacggct tcctcgacat cgaaactccg atgctgacca
     1021 aagccacgcc ggaaggcgcg cgtgactacc tggtgccttc tcgtgtgcac aaaggtaaat
     1081 tctacgcact gccgcaatcc ccacagttgt tcaaacagct gctgatgatg tccggttttg
     1141 accgttacta tcagatcgtt aaatgcttcc gtgacgaaga cctgcgcgct gatcgtcagc
     1201 ctgagtttac tcagatcgat gtggaaactt ctttcatgac cgcgccgcaa gtgcgtgaag
     1261 tgatggaagc gctggtgcgt catttgtggc tggaagtgaa gggcgtggat ctgggcgatt
     1321 tcccggtaat gacctttgcg gaagcagaac gccgttatgg ttctgataaa ccggatctgc
     1381 gtaacccgat ggaactgacc gacgttgccg atctgctgaa atctgttgag tttgcagtat
     1441 ttgcggggcc ggcgaacgat ccgaaaggcc gtgttgccgc tctacgtgtt ccgggtggcg
     1501 catcgctgac ccgtaagcag atcgacgaat acggtaactt cgttaaaatc tacggcgcga
     1561 aaggtctggc ttacatcaaa gttaacgaac gcgcgaaagg tctggaaggt atcaacagcc
     1621 cggtagcgaa gttccttaat gcagaaatca tcgaagccat cctggatcgt actgccgcgc
     1681 aagatggcga tatgattttc ttcggtgccg acaacaagaa aattgttgcc gacgcgatgg
     1741 gcgcactgcg cctgaaagtg ggtaaagacc ttggtctgac cgacgaaagc aaatgggcac
     1801 cgctgtgggt tatcgacttc ccgatgtttg aagacgacgg tgaaggcggc ctgacggcaa
     1861 tgcaccatcc gttcacctca ccgaaagaca tgactgctgc agaactgaaa gctgcaccgg
     1921 aaaatgcggt ggcgaacgct tacgatatgg tcatcaatgg ttacgaagtg ggcggtggtt
     1981 cagtacgtat ccataatggt gatatgcagc agacggtgtt tggtattctg ggtatcaacg
     2041 aagaggaaca gcgcgagaaa ttcggcttcc tgctcgacgc cctgaaatac ggtactccgc
     2101 cgcacgcagg tctggcattc ggtcttgacc gtctgaccat gctgctgact ggcaccgaca
     2161 atatccgtga cgttatcgcc ttcccgaaaa ccacggcggc agcgtgtctg atgactgaag
     2221 caccgagctt tgctaacccg actgcactgg ctgagctgag cattcaggtt gtgaagaagg
     2281 cagagaataa ctgatatgac tcaaatacac gaaatcattc gcgttgcatc aaggcggcaa
     2341 ttgagtgaac tcccatgagc atagataact atgtgaatgg gatgagcgaa ggcagtcaac
     2401 gaagaggcag cgtgaaggat aaagtgtata agcgtcccgt ttcgatctta gtggtcatct
     2461 atgcacaaga tacgaaacgg gtgctgatgt tgcagcggcg tgacgatccc gatttctggc
     2521 agtcggtaac cggcagcgtg gaagagggtg aaaccgcgcc gcaagctgcc atgcgcgaag
     2581 taaaggaaga ggtcaccatt gatgttgtcg ctgaacaact gaccttaatt gacagtcagc
     2641 gcacggtaga gtttgaaatt ttttcacatt tacgtcatcg ctatgcgccg ggcgtgacgc
     2701 gtaatacgga atcatggttc tgtcttgcgc ttccgcacga gcggcagatc gttttcactg
     2761 aacatctggc ttacaagtgg cttgatgcgc ctgctgcggc ggcgctcact aagtcctgga
     2821 gcaaccggca ggcgattgaa cagtttgtaa ttaacgctgc ctgaacaggc agggctatct
     2881 ggagatattt ttatggcagg tcatagtaaa tgggccaaca ccagacatcg taaagctgcg
     2941 caggatgcta agcgcggtaa aatcttcact aaaatcattc gtgagctggt aaccgcggct
     3001 aagctgggcg gtggcgatcc ggacgctaac ccgcgtctgc gtgcggcggt ggataaagca
     3061 ctgtctaaca acatgacccg tgacacactg aaccgcgcaa ttgcacgtgg tgtgggcggt
     3121 gatgatgatg caaacatgga aaccatcatc tacgaaggtt acggtcctgg cggcacggca
     3181 atcatgattg aatgtctgtc tgacaaccgc aaccgtaccg ttgctgaagt gcgtcatgca
     3241 ttcagcaaat gtggcggtaa cctcggtaca gatggttccg tagcctatct gttcagcaaa
     3301 aaaggcgtga tcaccttcga gaaaggcgat gaagacacca tcatggaagc agctctggaa
     3361 gcaggtgctg aagacgttgt gacctatgat gacggcgcga ttgatgtcta cactgcatgg
     3421 gaagaaatgg gtaaagtgcg cgacgctctg gaagcggcag gtctgaaagc agacagcgcg
     3481 gaagtttcca tgatcccgtc caccaaagct gatatggatg cagaaaccgc accgaaactg
     3541 atgcgtctga tcgatatgct ggaagattgc gacgacgtgc aggaagttta ccataacggt
     3601 gaaatctctg atgagattgc agcgactctc tgatgaggcc tgctaaacag caaaacggag
     3661 acgcgtgatg gctattattc tcggcattga cccgggttcg cgcgtgaccg gctatggcgt
     3721 catccgccag gtaggcaggc aactgtccta cctgggtagc ggatgcatac gcaccaaggt
     3781 ggatgattta ccgtctcgtc tgaagctcat ctatgcgggc gtgacggaaa tcatcaccca
     3841 gttccagcca gattatttcg ccattgaaca agtctttatg gccaaaaatg ccgactcggc
     3901 tctgaaactg gggcaggcgc gcggcgtggc gattgtggcg gcggtgaatc aggaattgcc
     3961 ggtatttgaa tatgcggcac gtcaggtaaa gcaaacggtg gtaggtattg gtagtgccga
     4021 aaaaagtcag gtgcagcata tggtccgcac cttgctgaaa ctgcccgcta atccacaggc
     4081 ggatgccgcc gatgcgttag ctatcgcgat tacccattgt cacgtcagcc agaatgcaat
     4141 gcagatgagc gaatcgcggc tgaacctggc gagagggcga ctgcgttaag ttataccgcc
     4201 tcggtcagtt caggctgagg cgtttcaact ccctccgcgt gaatcaacac caaacccgga
     4261 tgatgcc
//
LOCUS       DAJVUJ010000236         4192 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.238, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000236 DAJVUJ010000000
VERSION     DAJVUJ010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4192
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.238"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(<1..547)
                     /gene="exoX"
                     /locus_tag="OQQ48_004009"
     CDS             complement(<1..547)
                     /gene="exoX"
                     /locus_tag="OQQ48_004009"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HCS1954584.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGK"
     gene            complement(571..1227)
                     /locus_tag="OQQ48_004010"
     CDS             complement(571..1227)
                     /locus_tag="OQQ48_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HCS1954585.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLACDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            complement(1329..1559)
                     /gene="holE"
                     /locus_tag="OQQ48_004011"
     CDS             complement(1329..1559)
                     /gene="holE"
                     /locus_tag="OQQ48_004011"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HCS1954586.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVKLSRLPYEPKLK"
     gene            1698..2072
                     /gene="yobA"
                     /locus_tag="OQQ48_004012"
     CDS             1698..2072
                     /gene="yobA"
                     /locus_tag="OQQ48_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HCS1954587.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            2076..2943
                     /gene="copD"
                     /locus_tag="OQQ48_004013"
                     /pseudo
     CDS             2076..2943
                     /gene="copD"
                     /locus_tag="OQQ48_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
     gene            2956..3297
                     /locus_tag="OQQ48_004014"
     CDS             2956..3297
                     /locus_tag="OQQ48_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HCS1954588.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(3679..>4192)
                     /locus_tag="OQQ48_004015"
     CDS             complement(3679..>4192)
                     /locus_tag="OQQ48_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCS1954589.1"
                     /translation="REAADQLPAWFPLAMDLALVTGQRREDITNMRFSDIYDDRLHIR
                     QIKTGMMIAIPLSLSLPVAGLRLGAVVEQCRMVSRGDYLISAGIRKNSPDGSIHPDGL
                     TKKFVAARKLTGIQFSENPPTFHEVRSLAGRLYKETCGEEFAQRLLGHTSEKTTKMYL
                     DEREKTYLLL"
BASE COUNT         1048 a         1011 c         1028 g         1105 t
ORIGIN      
        1 ctttgccacg gtatttgcca aaggtgaagg tcgtcatcaa cgacggacgt ccggtgatat
       61 cggccatctg ttctgccgtc cagccggagg tgttcataat atcgataagc aacgcggcgg
      121 tgatataaca gtcatacaac gcgcggtgat gatgcaggcc cggcggcgtc tgtacattga
      181 gcttccgtgt tttatataac gccatattgc tgtacttgat ccctggccac aaacgacggg
      241 ccagtttcat agtgcaaatc cactcaccgg gcatctcagg cagtacgcgg cggtcaaagc
      301 tggcgttgtg cgcgacatac cattcactac cgtaatagtg tgggatcaca tcttcaatcc
      361 acggtttatc ggcgaccatg gcttcggtga tgcgatgaat cgccatcgct tgtggactaa
      421 taggacgatc ggggcgcacc aggtggctca tggggttgac gatttttccg tcaatgacat
      481 caacagaggc aatctcaacg atccctccct gcaaaccgca ggtttctgta tcgataatgc
      541 gcaacatggc atgctccagg ccgaaaaagc ctagcgtaat ggaatgatat cgccttgcca
      601 accctgtgat gaacgctgac cgactaacaa taatgaacca cgatcggcgc gaacgatgag
      661 acgaccatat tcatcccata gtgcactgtt accacgggca tttgccatca gcacggcaat
      721 cgagtactga tgtgaaaaaa attgtaagcg acgggccgac gccagcaggt cgggttctcc
      781 cagacattgc cctgtagtaa acagtgaaca ggtcgggtcc atatccatac cttgcggttg
      841 ttcatcgacc accgtgatgg ttctggaacg acggcccaga catgcaccat gactctgatg
      901 gtagatccca ggcgtttttc gccacggtgc gaacaccgca attccacgaa taaagcgatc
      961 gttatattca acgggaaggc cggcaatgat ggtcattcgc caggtcgttg cggcataaca
     1021 aagcgggtct aacagtgaga gatcgggtgg ggcaggaagg gcacgtcgcg aataatcaca
     1081 cgccagtaaa gaaagcgatg gaaaaactaa cagctgacat tgttgcctgg cggcggccct
     1141 cacaaactcc agatgatgag ctacctgttc ggtgagtgag gttttacgag gctcatattg
     1201 cgctgctgca actttccaga acgacataat gacttccttt tcaaaatgac tgcaaattat
     1261 gggtgcggtt tagaaaatac tcataattta gcaggcgtta tgtaagaaag tgtaactctg
     1321 tataagtttt atttaagttt gggctcgtaa ggtaaacgtg acagtttgac cgaagccaaa
     1381 cggtgggcaa taagcctctc gcgaaaccag ctgcgcaaat gttcaggctg ttcacgttca
     1441 accgcttcag cgatcaccgg catattgtag cgttctttaa atgccacccc ggccgccgcc
     1501 aaatcgacat tcactttatc catttctgtt tgatccagtt tagccagatt cttcagcatt
     1561 cttaatctcc tcatcattcg cgggcaaaag ttactaagag tgagccagga tggcaagccg
     1621 gaattgatga tcctcgactc agacattaga caaggttatg ctctgtaaaa aagttattac
     1681 aaaaagtaat agataatatg gcttcaactg cacgctccct tcgctacgcg ctggcaatcc
     1741 tgaccacttc gttggtgaca ccttccgtct gggcacatgc gcatttaacg catcagtatc
     1801 ctgcggcaaa cgcgcaagtg acagctgcac cgcaggcaat cactttaaac ttctcggaag
     1861 gtgttgaaac aggattcagt ggtgcaaaaa tcacggggcc aaaaaacgaa aatattaaaa
     1921 cattaccggc gaagcgaaat gagcaggacc aaaagcaact gattgttcca ctggccgatt
     1981 cgctgaaacc cggaacctac accgttgact ggcatgttgt ttcggtggac gggcataaaa
     2041 cgaaaggaca ctacaccttt agcgtgaaat aaatcatgct ggcgtttacc tggatcgcgc
     2101 tgcgatttat ccatttcacc tcgctgatgc tggtttttgg cttcgcaatg tacggtgcct
     2161 ggctggcccc cttgacgatt cgtcgcttgc tggcgaagcg ttttctgcgt ttacaacagc
     2221 acgctgccgt ctggagtttg atcagtgcca ctgcaatgct cgccgttcag ggcgggttga
     2281 tgggaacggg atggacagat gtattttcgc caaacatttg gcaggctgtt ttgcaaacgc
     2341 agtttggtgg cgtctggtta tggcaaattg ttctcgccct cgtaacgttg cgcccttatg
     2401 caaccgcgga atatgccacg tttgctgttt atgctcacta ccgcgcaatt tattctgctg
     2461 gcgggagtgg ggcatgcgac gctgaatgaa ggggtaacgg cgaaaatcca tcagactaat
     2521 cacacgattc acctgatttg tgcggcggcg tggtttgggg gattactgcc ggtgctctgg
     2581 tgtatgcaac tcatcaaagg ccgttggcga caccaggcta ttcaggcgct gatgcgtttt
     2641 tcatggtgcg ggcattttgc ggtgatcggc gtactggcaa gcggggtgct tagtgcattg
     2701 ttgattaccg gatttccccc cacgcttacg acttactggg gccaactctt attgctcaaa
     2761 gccatcctgg tgatgattat ggtggtcatt gcgctggcta atcgttatgt tcttgtaccg
     2821 cgcatgaggc aggatgaaga tcgtgcggca ccctggtttg tgtggatgac taaactcgaa
     2881 tgggcgatag gtgcggtagt gctggtgatc atcagcctgc ttgcgactct cgaacctttt
     2941 tgatggatta cgaaaatgat gaaaaaaagt attctggcgt ttctgttact caccagttct
     3001 gcagcggcgc tggctgcacc gcaggtgatt accgtcagtc gttttgaagt gggtaaagac
     3061 aaatgggcgt ttaatcgcga agaggtgatg ctgacttgcc gaccgggtaa tgctttgtat
     3121 gttattaatc cgagtaccct cgtgcagtat cctttaaacg atatcgcaca aaaggaagtt
     3181 gccagtggga agaccaatgc ccaacccatt tcggtgattc agattgatga tcctaacaat
     3241 cccggcgaaa aaatgagtct ggcaccgttt atagaacgag ctgaaaaact ctgttaatta
     3301 cctaaaatag ccttttgatt tccaataaaa aaaccgcctc agttctttca ccagaacggg
     3361 cggttttaac atttcagctg atgaccacca cgctttttat tgaccatttt gcacgcaaac
     3421 tggaaaacct ggcgtcgtca tctattctta aagggcaagg caactaagcc tgcattaatg
     3481 ccaactttta gcgcacggct ctctcccaag agccatttcc ctggaccgaa tacaggaatc
     3541 gtattcggtc tttttttaat tgcatttaaa atcaattagt tgcaaatgtc tccccgaaat
     3601 ttactcgaat ttctgtattc cggtcttttt tggttatatc acaaccaaaa tacatttaac
     3661 aatccattta cgttaaaatc agagcagtaa gtacgttttt tctctctcat caagatacat
     3721 ttttgttgtc ttctccgatg tgtggccaag tagacgctga gcaaattctt ctccacatgt
     3781 ttctttgtac aatcgtccag ccaggcttct gacttcgtga aaagttggtg ggttttcact
     3841 gaactggata cctgttaatt ttctggctgc gacaaatttc tttgtcaggc cgtccgggtg
     3901 aatgctgccg tcagggctgt tttttctaat cccggcactg attagataat cccccctact
     3961 taccatgcgg cactgttcaa ctactgcacc aagtcgtaaa ccagcgacag gaaggctgag
     4021 tgacaggggg atagcaatca tcattcctgt cttaatttgc ctgatgtgga gacgatcatc
     4081 ataaatatca ctgaaccgca tattcgttat gtcttcgcga cgttgtcctg ttacaagggc
     4141 taaatccata gctaatggga accatgccgg aagttgatct gctgcctccc tg
//
LOCUS       DAJVUJ010000237         4183 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.239, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000237 DAJVUJ010000000
VERSION     DAJVUJ010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.239"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OQQ48_004016"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OQQ48_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCS1954590.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="OQQ48_004017"
     tRNA            complement(324..399)
                     /locus_tag="OQQ48_004017"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="OQQ48_004018"
     tRNA            complement(406..480)
                     /locus_tag="OQQ48_004018"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="OQQ48_004019"
     tRNA            complement(597..681)
                     /locus_tag="OQQ48_004019"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="OQQ48_004020"
     tRNA            complement(690..765)
                     /locus_tag="OQQ48_004020"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="OQQ48_004021"
                     /gene_synonym="panK"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="OQQ48_004021"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HCS1954591.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAAQRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="OQQ48_004022"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="OQQ48_004022"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HCS1954592.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="OQQ48_004023"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="OQQ48_004023"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCS1954593.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQYATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLLKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1078 a         1041 c          933 g         1131 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctggaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagcgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc gacacgatga agaaacagcc gaatggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttcttcttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctaggagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctcagcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctctg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ccaacgggca
     1381 acgcattcct tacattatca gtattgctgg tagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcgctat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaagtc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa actgttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcggaatg gattatccac acgatccaca tcatgtattt gtttctgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagacg tggtatatca accgtttcct
     1861 gaaattccgc gaaggggctt ttaccgaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagcgatta agactgccat gacattgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgtga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgcg gtagaagagg tcagactacg caaataattt gcaggggagc gaatactccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagtaataa agccccctgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgacagat aaggtgccaa tccttcttgt tcgaagagtt ccaacgcagc acgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcgagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacggcgca ttgccatgtt gatcccggct
     2461 ccaatgacta tttgcgccgc atcgccagtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc
     2581 agcttgcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gcccccggcg accacggcca gcctgctggt attctgcaac acaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggtactgat tcgtggagtc aataactggc
     2821 agcacggcta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 atgggctcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcccagcgt ttcacccaac
     3001 tgctcgccag agtgaaattc accgttcgct aacagggcaa tcaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc agccccaccc atttgcatcc cttttagctg gcactgatca ataagccaac
     3301 ctgctgccag ttttactgaa ccacccgcct gggggtaatt tggtgctgtt ggaaattgcg
     3361 ccagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc gcattaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttagcaga cgcagaccta cggctacaat ggcgaagcga tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgtcct tctgcggttg catactgcca ggcattgagt aattgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagtt atgacgacag ggagagtttg tag
//
LOCUS       DAJVUJ010000238         4168 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.240, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000238 DAJVUJ010000000
VERSION     DAJVUJ010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4168
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.240"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            142..2139
                     /gene="betT"
                     /locus_tag="OQQ48_004024"
     CDS             142..2139
                     /gene="betT"
                     /locus_tag="OQQ48_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004119118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HCS1954594.1"
                     /translation="MSENDTIPKKSTSQINKAVFFTSALLIFLLVAFAAVFPDVADKN
                     FKLLQQQIFTNASWFYILAVALILLSVTFLGLSRYGDIKLGPDHAQPDFSYHSWFAML
                     FSAGMGIGLMFFGVAEPVMHYLSPPVGTPETVAAAKEAMRLTFFHWGLHAWAIYAIVA
                     LILAFFSYRHGLPLTLRSALYPIIGDRIYGPVGHAVDIFAVIGTVFGVATSLGYGVLQ
                     VNAGLNHLFGVPINETVQVILIVVITGLATISVVSGLDKGIRILSELNLGLALLLLAL
                     VLCLGPTVLLLKSFVENTGGYLSELVSKTFNLYAYEPKSSNWLGGWTLLYWGWWLSWS
                     PFVGMFIARVSRGRTIREFVTGVLFVPAGFTLMWMTVFGNSAIYLIMNQGATDLANTV
                     QQDVALALFNFLEHFPFSSVLSFIAMAMVIVFFVTSADSGAMVVDTLASGGVANTPVW
                     QRIFWASLMGIVAIALLLAGGLSALQTVTIASALPFSVILLISIYGLLKALRRDLTKR
                     ESLSMATIAPTAARNPIPWQRRLRNIAYLPKRSLVKRFMDDVIQPAMTLVQEELNKQG
                     TISHISDAVDDRIRLEVDLGNELNFIYEVRLRGYISPTFALAAMDNDEQQTEQHRYYR
                     AEVYLKEGGQNYDVMGWNQEQLINDILDQYEKHLHFLHLVR"
     gene            2202..3479
                     /locus_tag="OQQ48_004025"
     CDS             2202..3479
                     /locus_tag="OQQ48_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HCS1954595.1"
                     /translation="MISRWKWMLKQTIKKLWFRATLFAIVAIITALLSILFKSMIPES
                     VSVKVGAEAVDNILNILASSMLAVTTFSLSIMVTAYGSATTNVTPRATRLVVEDVTTQ
                     NVLATFIGSFLFSLVGIIALNMGAYGERGRVILFIVTLVVIALILITLLRWIQHLTSL
                     GRVGETTAQVEQAAIETFIARARNPCLGGYPWLENNEQPKGTVAVYPKKIGYVEYIDM
                     VKLSKLLTNDPRHVYLVAQPGSFIHPSMPVLYLSQGQESSINTDLLETIIVSDARSFA
                     QDPRFCLSVMAEIACRALSPAVNDPGTAIDVIGRGVRILSTYAQNKSDEIEVKYPSVH
                     VAPLQNNDLLEDFFSPVARDGASMREIQIRVLKGLSMLSKGWPEIFAEAAHTLAFETL
                     EHATRADHIDSDRYLIKSTYYNLFSGEYSNKKK"
     gene            3725..3886
                     /locus_tag="OQQ48_004026"
     CDS             3725..3886
                     /locus_tag="OQQ48_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001548957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1954596.1"
                     /translation="MTGENRGRGRPRKTESTYADTRNDLIRSGLELLTQNGFLATGVD
                     AIVSTTDEK"
     gene            complement(3930..4168)
                     /gene="istB"
                     /locus_tag="OQQ48_004027"
                     /pseudo
     CDS             complement(3930..4168)
                     /gene="istB"
                     /locus_tag="OQQ48_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
BASE COUNT         1006 a          959 c         1049 g         1154 t
ORIGIN      
        1 tggtagtgtc cgctattgcc agtacacctc aacattccac catgcattcc gattaacgcc
       61 gcatagccag ttgaactttg ctactttgtg agaggtagta ccttctatcc agtgcgaatt
      121 taattaatgg aataaatgat tatgagtgaa aatgatacaa tcccaaagaa gtctacaagt
      181 cagattaaca aagcggtatt ctttacatct gctttgctaa ttttccttct tgtcgccttt
      241 gccgccgtat tcccggatgt cgccgacaaa aattttaaac tacttcagca acaaatcttc
      301 acgaatgcca gctggttcta catccttgct gtggccctga ttttactgag tgtcacgttc
      361 cttggactct cacgctacgg tgatatcaag ctgggcccgg accatgcgca gcctgatttc
      421 agctaccact cctggtttgc gatgcttttt tcggcaggga tggggatcgg cctgatgttc
      481 tttggcgttg ccgaacctgt aatgcattat ctttcgccac ccgttggcac tccagaaacc
      541 gttgcggcag ctaaggaagc aatgcgtctg acctttttcc actggggact gcacgcatgg
      601 gcaatttatg ccattgtggc gctgattctg gcgttcttca gttaccgtca cggtctgcct
      661 ttaactctgc gctccgcact ctatcccatt attggcgatc gcatatacgg acctgtagga
      721 catgcggttg atattttcgc tgttataggc acggtctttg gcgttgcgac atcactgggt
      781 tacggtgttt tgcaggtgaa tgccggtttg aaccatcttt tcggggtgcc catcaatgaa
      841 acggtgcagg taattctgat cgtggtcatc acggggttag cgacgatttc agtggtgtcc
      901 ggtctggata agggaatacg tatcctgtct gaactcaatc tgggtctggc tttgttgctc
      961 ctggcgctgg tcctgtgtct gggaccaacc gtgcttctgc tgaagtcatt tgtggaaaat
     1021 acgggcggtt atctttcgga actggtgagt aaaacgttca acctttacgc gtatgagccc
     1081 aagtcgagca actggctggg gggctggaca ttactgtact ggggatggtg gctttcatgg
     1141 tcgccgtttg tggggatgtt catcgcacgg gtctcccgcg ggcgaaccat tcgcgagttt
     1201 gtcaccggcg tgctgtttgt tcccgcgggt tttacgctaa tgtggatgac ggtgtttggt
     1261 aacagcgcga tctatctcat tatgaaccag ggggccacag acctcgccaa tactgttcag
     1321 caggatgtgg cgctggccct gtttaatttc ctggagcatt tcccgttctc ttctgtgctg
     1381 tcattcattg caatggcgat ggtcatcgtc ttctttgtaa cgtctgctga ttcgggggca
     1441 atggttgtgg atactctggc atcaggtgga gtggcaaaca cacccgtctg gcagcgaata
     1501 ttctgggcct cgctcatggg cattgttgca attgcgcttc tccttgccgg agggctaagt
     1561 gcgctgcaaa cggtgacaat agcgagtgca ttgcccttct cagtgatctt actaatatct
     1621 atatacggac ttttaaaagc tttgcgccgg gatttgacca agcgtgaaag cctgagcatg
     1681 gcgacaattg ctcctacggc tgcacgtaac ccaattcctt ggcagagaag gttacgcaat
     1741 atcgcgtatc tgccgaagcg atctcttgtg aaacgtttta tggacgacgt tatccagccc
     1801 gccatgacgc tggttcagga ggaactgaac aagcagggga cgataagcca cattagtgat
     1861 gcagtcgacg atcgtattcg tcttgaagtc gatttgggca acgagctgaa ttttatatat
     1921 gaagtgaggc ttcgcgggta tatctcaccg accttcgcgc tcgccgcaat ggataatgat
     1981 gagcagcaga ctgaacaaca tcgatattat cgcgctgagg tttatctcaa agaaggcggt
     2041 caaaattatg atgtgatggg ctggaaccag gaacagctga ttaatgacat actggaccag
     2101 tacgaaaaac acctgcactt cctgcacctg gttcgttaat agcaacatgc cgtcctgggg
     2161 gcggcaatta ttatcccggc cgcaatatga gggaatgcag aatgatttca cgctggaaat
     2221 ggatgctgaa gcagacaatt aaaaaactat ggttcagggc aacgttattc gcaattgtcg
     2281 cgataataac ggccctttta tcaattcttt ttaaatcaat gatacctgag tcggtttccg
     2341 tgaaggttgg tgcggaagca gtcgataaca ttctgaacat actggcatcg agtatgctgg
     2401 cagtgaccac attttcgctg agtatcatgg tcacagccta cggttcagcc actactaatg
     2461 tgactcccag agctacgcgt ttagttgttg aagacgtcac cacacaaaat gtactggcca
     2521 ccttcatcgg ttcttttctc ttcagtctgg tagggattat tgccctcaat atgggagctt
     2581 atggagaaag ggggagagtc attttattca ttgtcacact ggttgtcatt gccttaatcc
     2641 tcatcacatt gcttcgctgg atacagcatt tgacctctct ggggagggtt ggtgagacaa
     2701 cggcacaagt agaacaggcg gccatcgaaa catttattgc gagagcaaga aatccctgtc
     2761 tcggcggata tccatggctt gagaacaatg aacagccgaa aggaacggtt gcagtttatc
     2821 cgaagaagat tggctatgtt gaatatattg atatggtgaa acttagcaag ctgctgacca
     2881 atgatccccg tcatgtatac ctcgtggcgc agccaggcag tttcatacat ccgtccatgc
     2941 cagttttgta cctgagtcag ggccaggagt cgtcaatcaa caccgattta cttgagacga
     3001 ttattgtctc ggatgcacgt tcatttgctc aggatcctcg attttgtctt agcgtcatgg
     3061 ccgaaatagc ctgccgggcc ctttcccctg cagtgaacga tcctggaacc gccattgatg
     3121 tcattggcag aggtgttcgt atactttcca cttacgcgca gaataaatct gatgaaatag
     3181 aagtgaaata tccttcagta catgttgcac cacttcagaa taacgatcta ctggaagact
     3241 ttttctcacc tgtcgcgcgc gatggtgcca gtatgaggga gattcagata agagtcctta
     3301 aaggactgtc gatgctgagt aaaggttggc ctgagatatt tgctgaagcc gcacataccc
     3361 tggcatttga aacattagag catgcaactc gtgctgacca tatagattct gatagatatc
     3421 taataaaatc aacttattat aatttattta gtggcgaata ttctaataaa aaaaaatagc
     3481 tgcagagcaa gcaattcggt tgttggcaag tcaagggcaa agcattcgcg tgagaatggg
     3541 cgtatgcttt gtgccaaaag caaacgttgc taattctgca ctgttcagcg atggcgcagt
     3601 agcgacaggc actgccactg tccagtcggc aaaagcgaac actttgccac tgcggggata
     3661 aactgattga agctctcgcg caaaatcact tattcttttg acaataaaat gtaggagatg
     3721 ggtcatgaca ggcgaaaatc gtggccgggg aaggccgcga aagacagaaa gtacctatgc
     3781 agatactcgt aatgatctga tccgcagtgg ccttgaactg ctgacgcaga atggttttct
     3841 tgcgacggga gtcgatgcca ttgtgtcaac gacggatgaa aagtgatcca cttatatctc
     3901 caccaacggc ccaatattga tccaccgttt tactcaggat tagcttcagc tataaccccg
     3961 gcctttcgtt tctgtctgag tcgatagctt tctcctttga tttgaacgac atgtgagtgg
     4021 tgtaagatac ggtccagcat cgctgaggtc agtgctgcat caccggcgaa cgtttgatcc
     4081 cactgcccga acggcagatt ggatgtcagg atcattgcgc tcttttcgta acgtttagcg
     4141 atgacctaga agaacagttt tgcttctt
//
LOCUS       DAJVUJ010000239         4128 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.241, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000239 DAJVUJ010000000
VERSION     DAJVUJ010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4128
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.241"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(37..594)
                     /gene="ymfL"
                     /locus_tag="OQQ48_004028"
     CDS             complement(37..594)
                     /gene="ymfL"
                     /locus_tag="OQQ48_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmfL"
                     /protein_id="HCS1954597.1"
                     /translation="MGKHHWKIEKQPEWYVKAVRKTIAKLPGGYAEAADWLDVTENAL
                     FNRLRADGDQIFPLGWAMVLQRAGGTHFIADAVAQSANGVFVSLPDIEDVDNADINQR
                     LLEVIEQIGSYSKQIRSAIEDGVVEPHEKTAINDELYLSISKLQEHAALVYKIFCISE
                     SNDARECAAPGVVASIASGCGETNA"
     gene            complement(638..838)
                     /locus_tag="OQQ48_004029"
     CDS             complement(638..838)
                     /locus_tag="OQQ48_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS1954598.1"
                     /translation="MKAYWDSLTKEQQGELAGKVGSTQGYLRLVFNGYKKASFVLAKK
                     LEQCTSGAITKSDLRPDIYPKD"
     gene            929..1603
                     /locus_tag="OQQ48_004030"
     CDS             929..1603
                     /locus_tag="OQQ48_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HCS1954599.1"
                     /translation="MKTIHDIRRSNARKLRDGVGGNSSFATMIDREPTQTSRFMGDGA
                     TKNIGDSMARHIEKCFDLPIGWLDQEHQTTNITKKPEVSITNKQITLVPVISWVQAGA
                     WKEVGYSEVDLSTAETYPCPVPCGEMTYILRVIGDSMIDEYRPGDMIFVDPEVPACHG
                     DDVIALMHDTGETTFKRLIEDGTQRYLKALHPNWPEPYIKINGNCSIIGTVIFSGKPR
                     RYKIKA"
     gene            3132..3668
                     /locus_tag="OQQ48_004031"
     CDS             3132..3668
                     /locus_tag="OQQ48_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD family hydrolase"
                     /protein_id="HCS1954600.1"
                     /translation="MSFIQTLTGKKFNYINSTIDDIYIEDIAGALSNICRFAGHLPDF
                     YSVAQHSVLCSQLVAPEFAFEALMHDATEAYCQDIPAQLKRLLPDYKRMEEKIDAVIR
                     EKYGLPPVMSTPVKYADLIMLATERRDLGLDDGSFWPLLEGIPATEMFKVIPLSPGHA
                     YGMFMERFNELSELRKCA"
     gene            3659..4021
                     /locus_tag="OQQ48_004032"
     CDS             3659..4021
                     /locus_tag="OQQ48_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1954601.1"
                     /translation="MRMNVFEMEGFLRGKCVPRDLKVNETDAEYLVRKFDALEAKCAA
                     LENKVIPVSTELPPANESVLLFDANGEGWLIGWRSLWYTWGQKETGDWQWTFQVGDLE
                     NVNITHWAVMPKAPEAGA"
BASE COUNT         1256 a          898 c          878 g         1096 t
ORIGIN      
        1 tcagttgcga gcgacggtag tgtgttgtta aactgttcat gcgttagttt ctccacaacc
       61 agaagcaatc gacgccacga cgcccggagc tgcacactcg cgggcgtcat tactttctga
      121 aatgcaaaaa attttgtaga caagtgctgc atgctcctgc agcttcgaaa ttgagagata
      181 cagctcgtcg ttaattgctg tcttctcatg cggttccact acaccgtctt cgattgctga
      241 acgaatctgt tttgaataac tgccgatctg ttcaatgact tccagcagac gctggttaat
      301 atcggcgttg tccacatcct cgatgtcagg aagagacaca aagacgccat ttgcagactg
      361 cgccacagca tcagcaatga agtgagtgcc accagcacgc tgtaaaacca ttgcccatcc
      421 cagcgggaaa atctgatcgc catctgcacg aaggcggttg aataaagcgt tttctgttac
      481 atcgagccag tcagccgctt cagcgtaacc acccggcaac tttgcgatag tttttctgac
      541 agctttcacg taccactcag gctgtttttc tattttccag tgatgcttac ccacgattag
      601 cctcatcgtt ctgtggttaa aaattgaaag tgttctgcta atctttcgga tagatatccg
      661 gtcttaagtc agatttcgta attgcacctg acgtgcattg ctcaagtttt ttagccagca
      721 caaaactggc ttttttatag ccattgaaaa ccagccgtaa gtagccttgt gttgagccaa
      781 cttttccggc caactcaccc tgctgttctt tggttaaaga gtcccaatac gctttcatac
      841 aatatgtacc tccagtgtac acatcacatg attgaaatga accttcaaga tacttgtacc
      901 ttagcggtac aagggtttta atttcgttat gaaaacaatc catgacatcc ggcggtctaa
      961 cgccagaaaa ctgagagatg gtgttggcgg gaattcttcc tttgccacta tgattgatcg
     1021 cgagccaacc cagaccagca ggtttatggg agatggtgct actaaaaata tcggtgacag
     1081 catggcacga cacattgaaa aatgtttcga cctgcctatc ggatggctcg atcaagaaca
     1141 ccagacaacg aacatcacaa aaaaacctga agtttcaatc actaacaaac aaataacgtt
     1201 agtccctgtc atatcatggg tacaggccgg agcatggaaa gaagttggct attctgaggt
     1261 tgatttgagc acagcagaaa cttatccctg ccctgtaccc tgtggcgaaa tgacttatat
     1321 cttgcgggtg attggtgatt caatgattga tgagtaccgt ccgggagaca tgatttttgt
     1381 tgatcctgaa gtccctgctt gccacggtga cgacgttatt gcgttgatgc acgatacagg
     1441 cgaaaccacc ttcaagcgat tgatagaaga tggaacacag cgttatctca aagcattaca
     1501 cccaaactgg cctgagccct acattaagat taacggtaat tgctctataa ttggcactgt
     1561 gattttctcg ggaaaaccaa gaagatacaa aataaaggcc taatcaatat ttatgaacct
     1621 gcttcggcag gtttttttat acttgacaat gtaccccatg gacacacaat gtaccccatg
     1681 gacacacaat gtaccaaaac gaaacaacaa acaggcagga tgcccacgaa gtagccgcct
     1741 ggggcatatg aagtccaaga tgattcgtta gcaacaaaac gcccatagga cgcttcgctc
     1801 tttaacaatt tgaatatttt cagcgtcaat gatctgttta gctccctaca agtaaatgta
     1861 gcgtagcacc atgataaatc ctgctagtgt ttagttaaaa atcgcgcgag agcaagaacg
     1921 acgtgagggc aggcaatact gatagtgtta ttgctattag tttatcaaaa atatttagac
     1981 gacccagatg aatattttta atacatactc cccctaattt aacaggagta aatgttgatg
     2041 aataacgaga catctatact caacaattgt atgatatgaa caattatata acataatgta
     2101 ggaaaaataa aaatggattg gcatgacctt atatcaaagg taattatcgg acttgttaca
     2161 ggctcaatcg cagcttactt aactgcgcga tatgcactcg gccgttatta taaagaaaaa
     2221 tggtgggaca aaagaataga tttgtatacg taattagttg actgtgttta tcttctaaaa
     2281 aacacagctg actattggta taaacatgag ttacatcacg cttcacaatt cccaacctac
     2341 tttagttaca aatcaaaaga agaaaatgaa atccttagca aatctttctc taatgagctt
     2401 gaaaaactca gtagaatagg tgagctatct tcattattct taacagaaga gtgtggggat
     2461 aaaatccgtt cattcattga aaatgaagaa aatctattta tacaatgggt gtttgatagt
     2521 atagagttga tagatgccca caagtcaaga cttgataaat taaaagtatt actagacgat
     2581 attatctctg aagcaaagag agagcttaag gtcaataaaa aacataacag cctaaagaat
     2641 ataaagtggt ccataactat gccggaccaa aaaaatgatg agccaattaa ttatcacgac
     2701 aatcattaaa acactttaga acccgctggg cgggttctat tcaaatatac tttgcacatc
     2761 acaaataaaa ctataacgtc cttcttcttt tcctaatatc ttatatctac ttatttataa
     2821 gcggcttaca gatcttcaac agataaacct aatgaaggcg acaatgttct gatgaatagc
     2881 caccttttta ctcaactgaa agcgcgttct gtcccttgtc attaagtgcc agttcgttaa
     2941 gtccaaaacc tccggaacgc gctttcaatt gagtggggaa aataactggc gatggcagcc
     3001 gcccgttgca ctaagtgcct ctggatgggg catttactga aacgaaacca aaacttttaa
     3061 tcgccttatg gcgagggatt cgtgcaacca aaaatcgtgc ggtgcagcgc accaattaag
     3121 gagaactaac gatgagtttt atccagactc ttaccggtaa gaagttcaat tatatcaact
     3181 ccaccattga cgatatttat atcgaggata tcgccggtgc gctttcaaat atctgccgct
     3241 tcgcgggaca cttgccggat ttttacagcg tggcgcagca ttccgttctg tgcagtcaac
     3301 ttgtggcgcc agagttcgcc tttgaagcgt taatgcatga tgcaacagaa gcgtattgtc
     3361 aggacatccc cgcgcaactg aaacgccttc ttcctgacta taaacggatg gaagaaaaaa
     3421 tagacgccgt aatccgtgag aaatacgggt tacccccggt tatgagcacg cccgtgaaat
     3481 atgccgatct catcatgctg gcaaccgaac gccgcgatct tgggcttgat gatggctctt
     3541 tctggcctct actagaaggt atcccagcaa cagagatgtt caaagttatt ccactgtcgc
     3601 caggccatgc ctacgggatg tttatggaac gttttaacga attatcggag ttacgcaaat
     3661 gcgcatgaat gttttcgaaa tggaagggtt tcttcgcggg aaatgtgtac cgcgagatct
     3721 gaaagtgaat gaaacggatg ctgaatacct ggtgcgtaaa ttcgatgcgc ttgaagctaa
     3781 atgtgcggca ctggaaaaca aagtaatacc agtgtcaact gaactgccac cagcaaatga
     3841 aagtgttttg ttattcgatg ctaacggaga aggctggcta attggctggc gttctctctg
     3901 gtacacctgg ggacaaaaag aaaccggaga ctggcagtgg acatttcagg tcggggacct
     3961 tgaaaacgtc aatatcactc actgggcagt aatgccaaaa gcaccggagg ctggagcata
     4021 atgaccactt ttaccgacaa agaactgatt aaagaaatta aagagcgtat cagcagcctt
     4081 gacgtgcgag acgatattga gcgccgtgct tatgaaatcg cactccta
//
LOCUS       DAJVUJ010000240         4080 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.242, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000240 DAJVUJ010000000
VERSION     DAJVUJ010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4080
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.242"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            33..932
                     /gene="pgrR"
                     /locus_tag="OQQ48_004033"
     CDS             33..932
                     /gene="pgrR"
                     /locus_tag="OQQ48_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HCS1954602.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALASLSELQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            1269..2882
                     /gene="mppA"
                     /locus_tag="OQQ48_004034"
     CDS             1269..2882
                     /gene="mppA"
                     /locus_tag="OQQ48_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HCS1954603.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRIMYIVKH"
     gene            complement(2933..3964)
                     /gene="ynaI"
                     /locus_tag="OQQ48_004035"
     CDS             complement(2933..3964)
                     /gene="ynaI"
                     /locus_tag="OQQ48_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HCS1954604.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
BASE COUNT         1145 a          896 c          960 g         1079 t
ORIGIN      
        1 taaatgatta atagattgca tggagatact gaatgaagcg tgaagaaatc gctgatctga
       61 tggcgtttgt cgtcgttgca gaggagcgta gcttcactcg tgcagcagcc cgcctgagca
      121 tggcgcagtc agctttaagc cagatagtgc gtcgtataga agaacgattg ggattgcggc
      181 ttctgacgcg aaccacgcgc agcgttgttc caactgaagc gggcgagcat cttttgtctg
      241 ttcttggccc tatgttgcat gacatagatt cagccctggc atccctgagc gagctgcaga
      301 accgcccatc cgggacaata cgtattacta ctgtagaaca tgcagcaaaa acgatattgt
      361 taccagcaat gcgcacattc ctgaaatcgc atcctgaaat tgatattcag ctcaccattg
      421 attatggttt gaccgatgtc gtttctgaac gttttgatgc aggcgtccgt ctgggtgggg
      481 agatggataa agatatgatc gccattcgaa tcgggccaga tataccaatg gctattgttg
      541 gctcaccgga ttatttttct cgccgaagtg ttccaacgtc agtgtcacaa ttaatagatc
      601 atcaggcaat taatttgtat cttcccacat cgggtacagc aaatcgctgg agattaatac
      661 gcggtggacg tgaagttcgt gttcgcatgg aaggtcagct tttactgaat acgatagacc
      721 tgatcattga tgctgcaatt gatgggcatg gattggcgta tctaccttat gatcaggttg
      781 agcgggctat taaagaaaaa aaactgatac gtgttttgga taaattcaca ccagatttac
      841 ccggttatca cctgtactat ccacaccgtc gacatgctgg ctcggcattc tcattattta
      901 tagataggct gaagtataaa ggtgctgttt agcactactt gctgatacat taatttaatt
      961 tctctcttaa cgtattctca gttcctttca acgttttggt tattttttat tcttcgtaca
     1021 atggcgacag atgctgattt tgataccgaa aactggtttg aacgtgcgaa gcctgaacgt
     1081 agtattcggt gttctatgtg ctttgccgca ttttggcgtc gattatttac actaaatctg
     1141 atttaatata ttgatacata aaacatttta tgcttcctgt gtcacttttt tgatggaagt
     1201 tgtttgcatt tctttaaggc gaaacaaata attacgcatc aattttaatg tcggttagag
     1261 ggaaacttat gaagcactct gtttcagtca cgtgttgtgc gctgttggtc agcagcattt
     1321 ctctttcgta tgctgcagaa gttccgagcg gcacagtact ggcagagaag caggagctgg
     1381 tgcgccacat taaagatgag cctgcgtcgc tggatcccgc taaagccgtg ggcctgccag
     1441 agattcaggt cattcgcgat ctgtttgaag gactggtgaa tcagaacgag aaaggggaga
     1501 ttgtccccgg cgttgcgact cagtggaaaa gtaatgacaa ccgtatctgg acttttaccc
     1561 tgcgcgataa cgcaaaatgg gcggatggca caccggtaac ggcgcaagat tttgtctaca
     1621 gctggcagcg tctggtggac ccgaaaacgc tatcgccatt tgcatggttt gccgcgctgg
     1681 cgggaatcaa caacgcacag gcgattattg atggtaaagc tacgcctgac cagcttggcg
     1741 tcaccgcagt tgatgcccat accttgaaaa ttcagcttga taaaccgttg ccgtggtttg
     1801 tgaatttaac cgctaacttt gccttcttcc cggtgcaaaa agccaacgta gaaagcggta
     1861 aagagtggac gaaacccgga aatctgatcg gcaatggcgc ttatgttctt aaagaacgcg
     1921 tagtcaatga aaaactggtc gtggtaccga atacccatta ttgggataac gccaaaacgg
     1981 tactgcaaaa agtgaccttc ctgccaatta atcaggaatc cgcagccact aagcgttacc
     2041 tcgcggggga tattgatatc accgaatcct tcccgaaaaa tatgtatcag aagctgttga
     2101 aggatattcc ggggcaggtt tatacgccgc cgcagctcgg gacctattat tatgcgttta
     2161 acacgcaaaa agggccgacg gcagatcagc gcgttcgcct ggcattaagt atgacgatag
     2221 atcgccgcct gatgaccgaa aaagtgttgg gaacgggcga aaagccagcg tggcatttta
     2281 caccagatgt taccgccgga tttacgccgg aaccttcgcc gtttgaacaa atgagtcagg
     2341 aagaactgaa tgcgcaggca aaaactttgt tgagcgcagc tggttatggt ccgcaaaaac
     2401 cgctgaagct gacgcttttg tataacactt cagaaaacca tcaaaaaatt gcgattgctg
     2461 tagcatcgat gtggaaaaag aaccttggcg tagatgttaa attgcaaaat caggaatgga
     2521 aaacctatat cgatagccgt aacaccggca attttgatgt tatccgcgcc tcatgggtgg
     2581 gggattataa tgaaccctcc actttcctga cattattaac gtcaacgcat tcaggaaata
     2641 tttcacgctt taacaatccg gcatacgaca aagttctggc ccaggcatcg acggaaaata
     2701 ccgttaaagc gcgtaatgcc gattacaacg cggcagaaaa aatcctcatg gagcaagcac
     2761 cgattgcacc aatttatcaa tataccaatg gacgattaat caagccgtgg ctgaaaggtt
     2821 atcccattaa taacccggaa gatgtggcgt acagccggat tatgtatatt gtgaagcatt
     2881 gatgtgatgg gaactggcgt tacccttgtg gataacgcca gtgatgtctg atttagcgcc
     2941 cctgttccgg cggtgtaatg ttatccatat acagcgtctg gctggggaag gcaaagtccg
     3001 cgccgtgtga ctgcacaata tcgataatct tcaaataaac gtcttgttgt gcagcaagcc
     3061 attcagccca taccgtggtt ttggtaaagc aataaaccat aatattcaat gacgagtcgg
     3121 caaactggtt gaaataaacc agtaaggttt gtcgctggtc gatggccggg tgatttttca
     3181 gcatctcacg tacggcttcg acaataacgc ccacttttgc cgcatcctca taacgtaaac
     3241 caatggtcgt ggtaatgcgg cggttggtca ttcgtcctgg gttttctacg ctgatcgacg
     3301 aaaacagcga gttcggtacg tacaatggac gattatcaaa ggtcgtaatt ttggtaattc
     3361 gccagccaat ttccgctact gtaccttcga tatttctgtc cggtgaacgg atccagtcgc
     3421 caatactgaa aggacggtcg aaatagagca tgatcccgga aaagaaatta ctcagaatat
     3481 ctttaccggc cataccgaca gccagaccac caataccacc aaaggtcagc aagccagaaa
     3541 ggctcatgcc gaaatgttcg ccataaagca gaacaagcac cacaataatg gtgattttga
     3601 taatgcgcga cataatccgc gcactggtga tatcgcgacc ttttttaatc tgctgttttt
     3661 caaactgatt aatcagcaaa aatagcttaa tcgtcagaat aaccgcaatc agggacgtac
     3721 agataaaatc gataacgcct ggggtgataa atttgagttt atagttttct ataacataat
     3781 taataatgct accaacagca ctgataatta tggtgtagat taaaaattgc accgcatgga
     3841 ataaaaaacc ttttctttta cgatttccgc ggcgaaacca aaagctcatc agaatcaatg
     3901 ctgcgcagct accgaaaata atgaccagat taagcgcatt atttgtaaac agttcagcga
     3961 tcattgtttt atcaggctcc tccagataat tgtcgtcatg ccggaacccc ctggcggggc
     4021 tattttaccg cgacaattca ttcagatcat caatagtcag ggaaggaagt agcaacatta
//
LOCUS       DAJVUJ010000241         4060 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.243, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000241 DAJVUJ010000000
VERSION     DAJVUJ010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4060
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.243"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(<1..1046)
                     /locus_tag="OQQ48_004036"
     CDS             complement(<1..1046)
                     /locus_tag="OQQ48_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HCS1954605.1"
                     /translation="MREINMLRNISSCLFPHISKITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRA"
     gene            1537..1647
                     /locus_tag="OQQ48_004037"
     CDS             1537..1647
                     /locus_tag="OQQ48_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
                     /protein_id="HCS1954606.1"
                     /translation="MLIGYVRVSTNDQNTDLRRNALNCAGYELIVERMGA"
     gene            1927..2922
                     /gene="pgl"
                     /locus_tag="OQQ48_004038"
     CDS             1927..2922
                     /gene="pgl"
                     /locus_tag="OQQ48_004038"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HCS1954607.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTIEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHLISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(2963..3915)
                     /locus_tag="OQQ48_004039"
                     /pseudo
     CDS             complement(2963..3915)
                     /locus_tag="OQQ48_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
BASE COUNT         1086 a          828 c          951 g         1195 t
ORIGIN      
        1 aatgcccggg aattctcctc ggtggaaaaa gaagtccatt gtgtagaaac ctcccgacga
       61 tctgcttcag gaaaccaggc tgccacagtt tcgggaagag gggggatctg aaagttctgc
      121 cccccttcag tgatgactct gatatggcgc gaatgtaaat tttgcggcaa catctggcgt
      181 aaaaacagtc tgttttggtc tgacagggaa atacgaaact gaggcccgtt aaaatttgga
      241 accgaagttt gctgtattaa gaatagcaga gtttgatctg ataaggggtt atttgcaatc
      301 tcaatagtta tatgaggatc taaacgaaaa agatttgtcg ggattgtagt tatttggttg
      361 ttttcaaaat aaaaaaatct tctcatgata atattttctt ggatgtttgg taagttgctt
      421 aatctattgc tatatgcaaa aagaagttgc agtctcctag gtaaaggggg cagttcagta
      481 agatggttgt gcgcaacctt aagtatctta agattagaag gcagagatgg aagagtttgc
      541 agttcgttgt tcctaacctc aagttttttt aaattcaagg gtaatgaagg cagtgtggat
      601 aacctattat actctgcgct tagtgatttg atggtgtctg gtaataacgg cagattctct
      661 aattgatttt cggacacatc caaagttatt agataatgtg gtaatgcaga tatatgtgtt
      721 aagaaattat ctcttgcaat aagttcttta atattttcag gtaaatcagt aaggatggtt
      781 aatttattac agctcacatc aagcttttta atgcaagcag ggagtacagg taacgaggaa
      841 agcgccaagg ctcgcaggtc cagtttatgc cccttagagg taagacatgc acgaagtctt
      901 cttaccgcct cagtacgctg ttcttccggt aacccctgtt tctcccaatc atcccattcg
      961 gacaaataat ggttggggga tgtaattttg ctgatatgtg gaaataaaca ggatgaaata
     1021 tttctgagca tattaatttc tcgcatatgg atatggaatt agattgtgaa taaattgtat
     1081 ttaatgtatc tctcctaatg aggagggtgt ttcagaatat tcgccataag ggaatgagtg
     1141 ttatatcatg ctttctatcc tggtaagtat atttgctttt ttcgtatatt tggttcatct
     1201 ttttttgatg ttttacatat tcacaatgca gaggatttat accagaagac attagtcttg
     1261 agtaagatac tacaacatct ccaactattt tagccagcct gactacttga cttggtaata
     1321 tttgttgcta ataatcatgt ataaaataag cttatataca tagtaacttc agcaaaggat
     1381 ttagtaatga tccaaatcat atatcaatgt actgtttttt tctttaaaaa aacagtacat
     1441 tctattttat aatgactgtg tattcccatg gaaggagatc acccaattct cccccccgac
     1501 ttttgacgca gtgtcttgtt aacaggggga tttaccatgc ttattggcta tgtacgcgta
     1561 tcaacaaatg accagaacac cgatctacga cgtaatgcgt tgaactgtgc aggatatgag
     1621 ctgattgttg agcgaatggg agcttgattg gaagttgtta gggtatggtg tttacggtcg
     1681 ttgatatggc gaacaaacgc ggggagcgtc acgctcccta aatggtcgcg aaaatatctg
     1741 tggcattgtc aaatttgtga cggtgactaa tattttaatg atatgccatt cgaaatggtg
     1801 tgctaacgta ttgatttaat tgtgacgcga tgttgtattt tatcgcgttt tgttgtctgt
     1861 ttttcattcg cttgatgttg ctatttacat gaaaaataaa gaatttattg tttttgaggt
     1921 gtgtttatga agcaaacagt ttatatcgcc agccctgaga gccagcaaat tcacgtctgg
     1981 aacctgaatc atgaaggcgc actgacgctg acacaggttg tcgatgtgcc ggggcaggtg
     2041 cagccgatgg tggtcagccc ggacaaacgt tatctctatg ttggtgttcg ccctgagttt
     2101 cgcgtcctgg cgtatcgtat tgccccggac gatggcgcac tgacctttgc cgcagagtct
     2161 gcgctgccgg gtagtccgac gcatatttcc accgatcacc aggggcagtt tgtctttgta
     2221 ggttcttaca atgcgggtaa cgtgagcgta acgcgtctgg aagatggcct gccagtgggc
     2281 gtcgtcgatg tggtcgaggg gctggacggt tgccattccg ccaatatctc accggacaac
     2341 cgtacgctgt gggttccggc attaaagcag gatcgcattt gcctgtttac ggtcagcgat
     2401 gatggtcatc tggtggcgca ggaccctgcg gaagtgacta ccattgaagg ggccggcccg
     2461 cgtcatatgg tattccatcc aaacgaacaa tatgcgtatt gcgtcaatga gttaaacagc
     2521 tcagtggatg tctgggaact gaaagatccg cacggtaata tcgaatgtgt ccagacgctg
     2581 gatatgatgc cggaaaactt ctccgacacc cgttgggcgg ctgatattca tatcaccccg
     2641 gatggtcgcc atttatacgc ctgcgaccgt accgccagcc tgattaccgt tttcagcgtt
     2701 tcggaagatg gcagtgtgtt gagtaaagaa ggcttccagc caacggaaac ccagccgcgc
     2761 ggcttcaatg ttgatcacag cggcaagtat ctgattgccg ccgggcaaaa atctcacctc
     2821 atctcggtat acgaaattgt tggcgagcag ggactactgc atgaaaaagg ccgttatgcg
     2881 gtcgggcagg gaccaatgtg ggtggtggtt aacgcacact aaccgctgat ttaaccggcg
     2941 cagtctctcc tgcgccggtg tattaaccta tctcctgtaa cgcatgtctc tggcgttcga
     3001 cgatattggt ccacaaattg tctttatcgt cagtccacaa attaatcagc aaggcaaaaa
     3061 agcgttcagc tgccggagaa agcacggcat ctttgcggcg aataatcccc aatgtccgac
     3121 gtataacggg ttccaccagt gggataccaa taatcgaaga atagggcgca tggggcattg
     3181 ccagaccagg aagcgccgaa atccccagtc ctgcctccac tagtcctaat gacgtcgaaa
     3241 gatgacgcac ttcgtaaaac cagtccagct tccagggctt gtcggccagc tgttgttcta
     3301 tcaacagtcg gttgccgctg gaggagcgta cgccaatcat tttgtaaccc actaactcct
     3361 gccattctac aagctgcttt ttggccaatg gatggtcacg ccgacaggca gcacgaacgg
     3421 ctcgttgacc agtggggtaa aatcaatgga tgaatttgtg acgttgttca tgtttatgcc
     3481 aaagtctgat tcgttacaca gcaccgattc catgcaatta ttcgtgcctt gttccagaat
     3541 ccgcacttta atattgggat acagctcatt aaatttaccg attgccagcg gtaaaaaata
     3601 aaatactgcg gtcggaatac acgctaacgt caccatacca cgatgataag cattcatatc
     3661 acgaatatta aaaagcgttt catcgaattt ctttattaat tcccgcgcct ccgggagcaa
     3721 cctttttcct gcttttgtta acgtaacctt gcgagttgta cgctcaaata gttgaatatg
     3781 taaatcttcc tccatctttt ttattcttcg cgttaatgca ggttgcgtaa tattgagtaa
     3841 tttagcggca ttgttaaacg aactggactc tgccagtatg acaaatgcct tcatacttga
     3901 taattcatgc ttcattatta ctccggaaaa tggaagcgac gattttgggt ggctggccgt
     3961 taaaaatttt aactgcattt agccaactta aattaatgaa aaaatgttat taatcgttga
     4021 gctaaagtca ttagagatgc tttgccctta atgtaaccat
//
LOCUS       DAJVUJ010000242         3901 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.244, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000242 DAJVUJ010000000
VERSION     DAJVUJ010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3901
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.244"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(303..722)
                     /locus_tag="OQQ48_004040"
     CDS             complement(303..722)
                     /locus_tag="OQQ48_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS1954608.1"
                     /translation="MVHEDKARKEFASRLALACENAGYEQHGRQAEIARRMKLTPKAV
                     SKWFNGETIPRREKLRELATLIGTTPTYLLGEDTEESGQVRFYQELNPRQKIIIDLLD
                     ELPDSETDELLKTLEEKKQKYNAIYEELARKKKQKAS"
     gene            802..1056
                     /locus_tag="OQQ48_004041"
     CDS             802..1056
                     /locus_tag="OQQ48_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCS1954609.1"
                     /translation="MNQKTLEDVIKTVRVAVVADVCGVSQRAIYKWMDNGKLPRTEYT
                     GETNYAEKIALASNGLFSADAILTIGRNKTTTKKLMGVDS"
     gene            1053..1478
                     /locus_tag="OQQ48_004042"
     CDS             1053..1478
                     /locus_tag="OQQ48_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCS1954610.1"
                     /translation="MKIKHEHIRMAMNAWAHPDGEKVPAAKITKAYFELGMTFPELYD
                     DSHPEALARNTQKIFRWVEKDTPDALKKIQALLPAIEKAMPPLLVARMRSHSSAYFRE
                     LVETRERLVRDADDFVAVAIAGFNQMNRGGPAGNIVAVH"
     gene            1550..2620
                     /locus_tag="OQQ48_004043"
     CDS             1550..2620
                     /locus_tag="OQQ48_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HCS1954611.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTRGHIDVTTEDLASALDVTEEVIDSILQTMQGRVLDGDLITGWEKRQVLKEDNGN
                     ISQTAKSPAERKRAQRERERKREQNGDCHGASRYVTHMSRRVTTDKDTDKDTDQEDQN
                     TMVHGVKNATNQAGDVQTVNPGQPAGTTPEADSAYALKADSGAVQQAMTASPEQSHQL
                     QQPEADSAIQREADRVVPENTGQSVGRVDYPDVFEQVWREYPLRAGANPKKSAFSAWK
                     ARLREGVPPEAMLDGVRRYARYLAATGKTGTEFVQRATTFFGPDRNFENPWLLPVSGT
                     NNQRCVNHISEPDNEIPPGFRG"
     gene            2661..3083
                     /locus_tag="OQQ48_004044"
     CDS             2661..3083
                     /locus_tag="OQQ48_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCS1954612.1"
                     /translation="MAKVFTQEEREKIKGQVLELVRQSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKLKKPSAVDPDLIWSLPDG
                     EIRRYDSRLNIICRECRKSEVMQRILSFYQGNFQEVMA"
     gene            3080..3385
                     /locus_tag="OQQ48_004045"
     CDS             3080..3385
                     /locus_tag="OQQ48_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HCS1954613.1"
                     /translation="MRVRVYIAGPMTGYKNFNREAFHNAEEELKREGHTVLNPAVLPD
                     GLTQPQYMDICMAMIRCVDAIYMLKGWQRSAGAKAELALAEKLGHAVIFQETASVQD"
     gene            3372..3827
                     /locus_tag="OQQ48_004046"
     CDS             3372..3827
                     /locus_tag="OQQ48_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HCS1954614.1"
                     /translation="MCKTNYQALRERYSPIQVPECPVCGDEMSIQRIFSRTHIVYACT
                     GEGDDGYFKTGRTFADEHYLKSRVTVVDVSAPDVLALLKELEVKDKRIAELTDALTQM
                     INAHKTTIRFGHERITECGGDCDSPEKMISENPDIRMAEAVLRAGIKTE"
BASE COUNT         1067 a          841 c         1047 g          946 t
ORIGIN      
        1 tccatgctga cctcatttcc ccttaacgcc ggggtagcgg aacaaaaacc tgctgcatag
       61 ttattaaagt tgaaccctgc cgtcatgttc ttacgcctcg ggctggctac ttaacccctg
      121 accactgcct ggtaactcga agtattgccc tgcattctgt ggggcggggt gggtggcagg
      181 tatataatgt actttgcgtt cattgttgta aagtgcttat ggtacatttt gtgtgtaaaa
      241 aaatgagatg ggataaagtg aagcacaaac ccggaggaag gcgctaccgg atttatgctg
      301 gtttaagagg ctttttgttt tttctttcgt gctaactctt cgtaaattgc attgtacttc
      361 tgttttttct cttcaagagt ttttaaaagt tcatctgtct cactgtcagg gagctcgtcc
      421 agaaggtcaa tgatgatttt ttgtcttgga tttaactcct gatagaaacg tacctgtcca
      481 ctttcttctg tatcctctcc caaaagatag gttggtgttg ttcctattag tgttgctaat
      541 tcccttaatt tctcccggcg aggaattgtt tcgccattaa accatttgct aaccgctttt
      601 ggtgttaatt tcattcgacg ggcaatttct gcctgccttc catgttgttc ataaccagcg
      661 ttttcacagg ctagcgcaag cctactggcg aactctttac gcgctttatc ttcatgaacc
      721 ataagttcaa tgatattcgc tcttgaatgt actgtcagtt ctgttatagc atgtactcaa
      781 agttcacatt gtgagggtga tatgaaccag aaaacacttg aagatgtaat caaaactgtt
      841 cgcgttgctg ttgtggccga cgtttgtggt gtcagccaaa gagcaatcta taaatggatg
      901 gataacggaa aattgcctcg cacagaatat accggcgaaa caaattacgc tgaaaaaatc
      961 gctcttgcat caaacggatt attttctgcc gatgcaattt taactattgg caggaataaa
     1021 actactacga aaaagctgat gggagttgat tcatgaaaat caagcatgaa cacatccgca
     1081 tggcgatgaa tgcctgggcg catccggacg gcgaaaaagt accggctgcg aaaattacca
     1141 aagcgtattt cgagctggga atgacgttcc cggaactgta tgacgacagc catccggaag
     1201 ccctggctcg caatactcag aaaattttcc gctgggtgga gaaagacacc cctgatgcgc
     1261 ttaaaaaaat tcaggcgttg ttaccagcta tcgaaaaagc aatgccacct ctgctggtgg
     1321 cccgaatgcg cagccatagc tcagcctatt ttcgggaact agtggagacg cgggaacgac
     1381 tggtgagaga cgctgatgat tttgtcgcag tggcgatcgc tggtttcaac cagatgaatc
     1441 gtggtggccc tgcaggaaat attgtggctg tgcattgact cgcaatattc ataccggatc
     1501 acttccggca atttgtgagt aaaaagattc ggtatcaaaa gaggtgagta tggctaacgc
     1561 ctggctcaga ttatggcatg acatgccaaa tgaccctaag tggcgaacaa ttgccagggt
     1621 gtcagggcag ccaattgcaa cagtgatggc agtgtatatc cacctcctgg tgagcgcgtc
     1681 acgaaatgtc acgcgaggtc acattgatgt cacgacagaa gatttggcaa gtgcgctcga
     1741 cgtgacagaa gaggtaattg attcaatttt gcagacgatg caggggcggg tacttgatgg
     1801 tgatttaatc actggatggg aaaaacgcca ggtgctgaaa gaggacaacg gcaatatttc
     1861 gcaaaccgca aaatctcctg cagagcgcaa gagggcgcag cgagagaggg aaagaaagcg
     1921 ggaacaaaat ggcgattgtc acggcgcgtc acgatatgtc acgcacatgt cacgacgagt
     1981 cacgacagat aaagatacag ataaagatac agatcaagaa gatcaaaaca ctatggtcca
     2041 tggcgtaaaa aacgccacga accaggcagg ggatgttcag accgtcaatc ctggtcagcc
     2101 agcaggcacg acaccggaag ccgattcagc gtatgcgctg aaagccgatt cgggcgctgt
     2161 gcagcaggcg atgaccgcaa gtccggagca atcacaccaa ctgcagcagc ctgaagccga
     2221 ttccgccatt cagcgggaag ccgatcgggt agtcccggaa aacaccgggc agtctgtggg
     2281 acgagtggat tatccggatg tgttcgaaca ggtctggcgg gagtacccgt tgcgtgccgg
     2341 ggcaaacccg aagaaatccg ctttcagtgc ctggaaggcc agattacgcg agggggtgcc
     2401 accagaggcc atgctggatg gcgtgaggcg ttacgcaaga tacctggcgg ctaccgggaa
     2461 aacgggaacg gaatttgttc agcgagcgac gacgtttttt ggaccggacc ggaattttga
     2521 gaacccctgg ttgctcccgg taagcggcac gaacaaccag cgttgtgtga atcatatttc
     2581 tgaaccggat aacgaaattc cgccgggctt cagggggtaa gtgttaattt ctggtcatga
     2641 ggtaattttc aggagggctt gtggcaaaag tttttacaca agaagagcgg gaaaaaatta
     2701 aagggcaggt tcttgaactc gtacgccaga gtgggcgcga gacgttacga caactggaag
     2761 ctaaaactgg ggcaacaaga tatctgatga gcgttctggc cagagagctg gttgccagtg
     2821 gcgatgtata caactctggt tacgggttat tcccgtctga acaggcgcgt aaggactggc
     2881 aaaatgcccg taaaaagctc tcaagggcaa agctgaagaa accatctgcg gttgatccgg
     2941 accttatctg gtcattacct gacggagaaa tacgtcgcta cgacagtcgc ctaaacataa
     3001 tctgtcgcga gtgccggaag agcgaagtta tgcagcgcat attgtcgttt tatcagggta
     3061 attttcagga ggtgatggcg tgagggtgag agtctatatc gccggtccaa tgaccgggta
     3121 taaaaatttc aaccgtgagg cgtttcacaa tgcggaagag gaactgaaac gggaagggca
     3181 taccgtctta aacccggcag tacttccgga cgggctgaca cagccgcagt acatggatat
     3241 ctgcatggcg atgattcgtt gtgtggatgc gatttacatg ctgaaaggct ggcagcggtc
     3301 agcaggcgct aaggcagaac tggcgctggc ggagaaactg gggcatgcgg tgatttttca
     3361 ggagacagcc agtgtgcaag actaattacc aggcattacg tgaacgttat tcaccaattc
     3421 aggtgccgga atgtcctgtt tgtggcgatg aaatgtcgat acagcgcata ttttccagaa
     3481 cgcatattgt gtatgcctgc acaggtgagg gggatgatgg atattttaaa actggtcgaa
     3541 cttttgcgga tgagcattac ctgaaatcgc gcgtaaccgt cgttgatgtt agcgctcctg
     3601 acgtactggc tttactgaaa gagctggaag ttaaagacaa acgcattgca gaattgacag
     3661 atgcgcttac acaaatgatt aatgcgcaca aaaccacaat tcgttttggt catgaacgca
     3721 taactgaatg tggtggtgat tgcgactcgc cggaaaagat gatttcagaa aatccggata
     3781 tcagaatggc agaggctgtt ttgagagcag gaataaaaac tgaataatta aatttagcac
     3841 agcaaataaa atttaatcct taaccggagg gatttctgca ccctcagaac atcaggaggc
     3901 c
//
LOCUS       DAJVUJ010000243         3853 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.245, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000243 DAJVUJ010000000
VERSION     DAJVUJ010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3853
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.245"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..409
                     /locus_tag="OQQ48_004047"
     CDS             <1..409
                     /locus_tag="OQQ48_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCS1954615.1"
                     /translation="FTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVR
                     ISIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIA
                     EGVESEDVARELIKLGCVQAQGYLYQKPMPFSA"
     gene            447..1775
                     /locus_tag="OQQ48_004048"
     CDS             447..1775
                     /locus_tag="OQQ48_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCS1954616.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGSYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKIYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(1822..3147)
                     /gene="rimO"
                     /locus_tag="OQQ48_004049"
     CDS             complement(1822..3147)
                     /gene="rimO"
                     /locus_tag="OQQ48_004049"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HCS1954617.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            3360..3743
                     /gene="bssR"
                     /locus_tag="OQQ48_004050"
     CDS             3360..3743
                     /gene="bssR"
                     /locus_tag="OQQ48_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HCS1954618.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
BASE COUNT         1007 a          857 c          949 g         1040 t
ORIGIN      
        1 ttttaccacc atcatgaaag aaaatggcct gaaggcgaac agcctctgtg ttgagatcac
       61 tgaaaccgtg atcgagcgaa ttaatgaaca tttttatctc aatattgaac aactgcgtaa
      121 acaaggggta cggatatcga ttgatgactt tggcaccggt ttgtcaaacc tgaaacgttt
      181 ttatgaaatt aatccagaca gcataaaggt ggactcgcaa tttaccggcg atattttcgg
      241 tactgcggga aaaattgtgc gcattatttt cgatctggca cgctataacc ggatcccggt
      301 gattgcggaa ggcgtagaga gcgaagacgt tgcacgcgaa ttaattaaat taggatgtgt
      361 tcaggctcag gggtatctgt accagaaacc tatgccattc tccgcctagg ataaaagtgg
      421 aaaattagta aaagagtagt ttacgtatgt ccagaatcaa taagttcgta cttacagtca
      481 gtctgctgat ttttatcatg atttcagcag ttgcctgcgg gatctacact caaatggtaa
      541 aggaacgggt gtatagcctg aaacagtccg ttattgatac tgcttttgcg gtggcaaata
      601 ttgctgaata tcggcgtagc gtggcaattg atcttatcaa cacgctaaat cccactgagg
      661 aacagctgtt ggttggtttg cgcacagctt acgccgactc ggtttccccc tcttatttgt
      721 acgatgtcgg tccttatctg atttccagtg acgaatgtat tcaggtaaag gagttcgaga
      781 aaaattattg tgcagatatt atgcaggttg tgaagtatcg acatgtcaaa aatacagggt
      841 ttatctcttt tgacggtaaa accttcgtct attacctcta tccggtaact cacaatcgta
      901 gtctgatatt tttgcttggt ctggagcgtt tttctttact gtcaaaatcg ctggcgatgg
      961 acagcgagaa cctgatgttc tctctattta agaacggtaa accggtgacc ggtgatgaat
     1021 ataatgctaa aaacgccatc ttcaccgttt cggaagcgat ggagcacttc gcctatttgc
     1081 cgaccggatt gtacgtattt gcgtataaaa aagatgttta tttgcgggtc tgtacattga
     1141 ttattttctt tgccgcattg gtggctgtga tatcgggtgc cagttgcctc tatctggtac
     1201 gcagagtgat taatcgtggt attgtggaga aagaagccat cattaataac cattttgaac
     1261 gcgtactgga tggcgggctt ttcttttcgg ctgccgatgt caaaaaactc tacagtatgt
     1321 ataactcggc gttcctggac gacctgacca aagcaatggg cagaaaatcc tttgacgaag
     1381 atttaaaagc gctgccggaa aaaggcagtt atttgtgcct gtttgacgtc gataaattca
     1441 aaaacattaa cgacaccttc ggtcatttgc tgggcgatga agtgttgatg aaagtggtga
     1501 aaatccttaa atcacagatc ccggtagata aaggtaaaat ctaccgcttc ggcggtgacg
     1561 aatttgcggt gatttatacg ggtggaacgc tggaagagtt gctatcgatt ctaaaagaaa
     1621 tcgttcattt ccaggtggga agcattaatt taagtaccag tatcggtgta gcgcattcaa
     1681 atgaatgtac caccgtcgaa cgcttgaaaa tgctggcgga tgagcggctg tataagagta
     1741 agaaaaacgg cagggctcag attagctggc agtaatcatt attcgcaggc cggacaaatg
     1801 attttgcccg gcctgaatta attaaacccg gctaccccac aaatcgtact catcggcgtg
     1861 ctcgactttc acacgcagga tatcacccgg cttaacgttg gtttcaccat tgagataaac
     1921 cgcgccgtcg atttccggtg catctgccat gctgcgacca atcgcgcctt cttcgtccac
     1981 ttcgtcgata atcaccagaa tttcacggcc cactttctct tgcaggcgct cggcggaaat
     2041 ctgctgctgc agctgcatga aacggttcca gcgttcttct ttaacttctt ccggaacctg
     2101 gtcaggcagg gcattggcgt ctgcaccttc aaccgggctg tatttaaagc agccaacgcg
     2161 atccagacgc gcttctttca ggaagtcgag tagcatctgg aaatcttctt ctgtctcgcc
     2221 agggaagccg acaataaagg ttgagcgtag ggtcagttcc gggcagattt cgcgccactg
     2281 tttgatgcgc gccagttggc gatctacaga acccggacgc ttcatcagtt tgagaatgcg
     2341 cgggctggcg tgctgcaacg gaatgtccag atacggcagg attttgcctt ctgccatcag
     2401 tgggatgacg tcgtccacat gcggataagg gtaaacgtag tgcagacgtg tccagatccc
     2461 cagtttcgat aactgttcgc acaggctgac catgctggtt tttaccggct cgccgttgtg
     2521 gaagccagta cgatgtttaa catcaacgcc ataggcggaa gtatcctgcg agatcaccag
     2581 aatctcttta acgcccgcat ctaccagacg tttcgcttca cttaacactt cgccaatcgg
     2641 acggctcacc aggtcgccgc gcatagacgg aataatgcag aaggtgcagc ggtgattaca
     2701 gccttccgaa attttcagat aggcataatg acgcggcgtc agtttcacac cttgttctgg
     2761 caccaggctc aggaatgggt tgtgtttcgg ttttggcacg tagtgatgaa cgtgctccag
     2821 aacctgctca tagctatgag gcccggtgat ttccagcact ttcgggtgga cttcgcggat
     2881 ctgatcttct tttgccccca gacaaccggt cacaattacc ttgccgtttt cattcaacgc
     2941 ttcaccaatg gcttccagtg attcttgtac cgcgctgtca ataaagccgc aggtgttgac
     3001 gatcaccatg tccgcatcgt catagctcgg taccacgtca taaccttcag tgcggagttc
     3061 ggtgagaata cgctctgaat cgacaaggtt tttcggacag ccaagggaaa caaagccgat
     3121 tttcggctgg ggagttactt tgctcatagc ttaaaaaata ttcagttaca ggaaaggtca
     3181 gggcagggat tctacagagt tctggataaa atttgtatcg caatctcatt cgctggcgga
     3241 ggcgaaggaa atgtaaattt tgttaattcg gcgtgaagaa ttgatcctgg acagcatttt
     3301 gctcaaaaaa tagccatact atttaattgc aacaaggctg gaaagaggag gatcgaagta
     3361 tgttcgttga cagacagcga atcgatctgc tgaaccggtt gatcgacgca cgcgttgacc
     3421 tcgctgcata cgtgcaactg aggaaggcaa aaggatacat gtccgtcagc gaaagcaatc
     3481 atctacgaga taactttttt aaactgaatc gcgaactgca cgataaatcg ctgcggttga
     3541 atcttcatct ggatcaggaa gagtggagtg ctcttcatca tgctgaagaa gcattagcga
     3601 cagccgcagt atgtttgatg agtgggcacc atgattgccc gactgttatt accgtcaacg
     3661 ccgataagct tgaaaattgt ctgatgagct taacgctgag tatccagagc ctgcagaagc
     3721 acgccatgct tgagaaggcc tgaaaactaa gggggagaaa gcgtctcccc cttcatgttt
     3781 aagtttttgt aaaaatgaat ttgttatctc ctccactgac tacgctttaa gccagagtca
     3841 atccggaggc gtt
//
LOCUS       DAJVUJ010000244         3830 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.246, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000244 DAJVUJ010000000
VERSION     DAJVUJ010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3830
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.246"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(1..270)
                     /locus_tag="OQQ48_004051"
                     /pseudo
     CDS             complement(1..270)
                     /locus_tag="OQQ48_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            259..666
                     /locus_tag="OQQ48_004052"
     CDS             259..666
                     /locus_tag="OQQ48_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCS1954619.1"
                     /translation="MRGRPVLFPNFSLGEEYEHAPPATNRQISPYLPSGRFRTGLPVE
                     GLAIERGDLFYACPRASVFYGTALDADLRTRGVSTLVMAGISTTGVVLSSVAWASDAD
                     YDVRLVQDCCYDPDRDAHEALLRSGFGGRVQVV"
     gene            804..1688
                     /locus_tag="OQQ48_004053"
     CDS             804..1688
                     /locus_tag="OQQ48_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HCS1954620.1"
                     /translation="MSLRTPDLLFTAIAPAIWGSTYIVTTQYLPNFSPMTVAMLRALP
                     AGLLLVMIVRQIPTGIWWMRIFILGALNISLFWSLLFISVYRLPGGVAATVGAVQPLM
                     VVFISAALLGSPIRLMAVLGAICGTAGVALLVLTPNAALDPVGVAAGLAGAVSMAFGT
                     VLTRKWQPPVPLLTFTAWQLAAGGLLLVPVALVFDPPIPMPTGTNVLGLAWLGLIGAG
                     LTYFLWFRGISRLEPTVVSLLGFLSPGTAVLLGWLFLDQTLSALQIIGVLLVIGSIWL
                     GQRSNRTPRARIACRKSP"
     gene            complement(1720..2919)
                     /gene="tet(A)"
                     /locus_tag="OQQ48_004054"
     CDS             complement(1720..2919)
                     /gene="tet(A)"
                     /locus_tag="OQQ48_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HCS1954621.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSG
                     AGQRADR"
     gene            3025..3675
                     /gene="tetR(A)"
                     /locus_tag="OQQ48_004055"
     CDS             3025..3675
                     /gene="tetR(A)"
                     /locus_tag="OQQ48_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HCS1954622.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGG
                     TVEQAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
BASE COUNT          719 a         1179 c         1202 g          730 t
ORIGIN      
        1 gaacttctcg gccggcagca ggtagtcgtc gaagtcgcgg aactgccgcg agcccttgac
       61 ccagatgtcg ccggagcgca gggcgttctt cagctcggac agggcgcaga tttcgtagaa
      121 tttccggtcg aggccttccg gggtgatcac cagcggcttc cagcgcggct tgatgaaggc
      181 cgtgggtgca tcggccggca ccttgcgcag gttgtcggcg ttcatctcac gcagggtctg
      241 cacggctgcc agcacgcctt gcgcggccgg cccgtgctgt tccccaactt ttcgctcggc
      301 gaagagtatg aacatgcgcc gccggcgacg aatcgccaaa tctcaccgta tctcccgagc
      361 ggacgatttc gcaccggtct gcccgtcgaa gggcttgcga tcgaacgggg cgaccttttc
      421 tatgcatgtc cgcgagccag cgtcttttat ggcacggcgc tcgacgccga ccttcggacg
      481 cgcggcgtaa gcacgcttgt catggccggg ataagcacca ccggcgttgt tctttcaagc
      541 gtcgcctggg ctagtgatgc ggactacgac gtgcgtttgg tccaggactg ctgctacgac
      601 ccggatcggg atgcccacga agctctgttg cgttccgggt tcggcggacg tgtacaggtc
      661 gtgtaatttt cgggcctcgc atgatcgcgg ccatagccgc aggtggattg gtgcgaggca
      721 gggccagtcc aagtcagggt gcggtcatcg cggcgcctgc aactctcaaa aacccatcgc
      781 cgtagcggag gtcttgtaat cgcatgtcat tacgcacgcc cgatttattg ttcacagcga
      841 tagcacctgc catttggggc agcacctaca ttgtcaccac ccaatacctg ccgaacttct
      901 caccgatgac ggtcgcgatg ctgcgggcgt tgccggcggg tttattgctc gtgatgatcg
      961 tccgacagat tccaacggga atctggtgga tgcgcatctt catcctcggc gcacttaata
     1021 tttcgctatt ctggagcttg ttgtttattt cggtctaccg cctgccgggc ggggtcgcgg
     1081 cgacggtagg cgctgtgcag ccgctgatgg tcgtgttcat ctctgccgct ctgctaggta
     1141 gcccgatacg attgatggcg gtcctggggg ctatttgcgg aactgcgggc gtggcgctgt
     1201 tggtgttgac accaaacgca gcgctagatc ctgtcggcgt cgcagcgggc ctggcggggg
     1261 cggtttccat ggcgttcgga accgtgctga cccgcaagtg gcaacctccc gtgcctctgc
     1321 tcacctttac cgcctggcaa ctggcggccg gaggacttct gctcgttcca gtagctttag
     1381 tgtttgatcc gccaatcccg atgcctacag gaaccaatgt tctcggcctg gcgtggctcg
     1441 gcctgatcgg agcgggttta acctacttcc tttggttccg ggggatctcg cgactcgaac
     1501 ctacagttgt ttccttactg ggctttctca gcccggggac cgccgtgttg ctaggatggt
     1561 tgttcttgga tcagacgctg agtgcgcttc aaatcatcgg cgtcctgctc gtgatcggga
     1621 gtatctggct gggccaacgt tccaaccgca ctcctagggc gcgtatagct tgccggaagt
     1681 cgccttgacc cgcatggcat aggcctatcg tttccacgat cagcgatcgg ctcgttgccc
     1741 tgcgccgctc caaagcccgc gacgcagcgc cggcaggcag agcaagtaga gggcagcgcc
     1801 tgcaatccat gcccacccgt tccacgttgt tatagaagcc gcatagatcg ccgtgaagag
     1861 gaggggtccg acgatcgagg tcaggctggt gagcgccgcc agtgagcctt gcagctgccc
     1921 ctgacgttcc tcatccacct gcctggacaa cattgcttgc agcgccggca ttccgatgcc
     1981 acccgaagca agcaggacca tgatcgggaa cgccatccat ccccgtgtcg cgaaggcaag
     2041 caggatgtag cctgtgccgt cggcaatcat tccgagcatg agtgcccgcc tttcgccgag
     2101 ccgggcggct acagggccgg tgatcattgc ctgggcgagt gaatgcagaa tgccaaatgc
     2161 ggcaagcgaa atgccgatcg tggtcgcgtc ccagtgaaag cgatcctcgc cgaaaatgac
     2221 ccaaagcgcg gccggcacct gtccgacaag ttgcatgatg aagaagaccg ccatcagggc
     2281 ggcgacgacg gtcatgcccc gggcccaccg gaacgaagcg agcgggttga gagcctcccg
     2341 gcgtaacggc cggcgttcgc ctttgtgcga ctccggcaaa aggaaacagc ccgtcaggaa
     2401 attgaggccg ttcaaggctg ccgcggcgaa gaacggagcg tggggggaga aaccgcccat
     2461 cagcccaccg agcacaggtc ccgcgaccat cccgaacccg aaacaggcgc tcatgaagcc
     2521 gaagtgccgc gcgcgctcat cgccatcagt gatatcggca atataagcgc cggctaccgc
     2581 cccagtcgcc ccggtgatgc cggccacgat ccgcccgata tagagaaccc aaaggaaagg
     2641 cgccgtcgcc atgatggcgt agtcgacagc agcgccggcc agcgagacga gcaagaccgg
     2701 ccgccgcccg aaacgatccg acagcgcgcc cagcacaggt gcgcaggcaa attgcatcaa
     2761 cgcatacagc gccagcagaa tgccatagtg ggcggtgacg tcgttcgagt gaaccagatc
     2821 gcgcaggagg cccggcagca ccggcataat caggccgatg ccgacagcgt cgagcgcgac
     2881 agtgctcaga attacgatca ggggtctgtt gggtttcacg tctggcctcc ggaccagcct
     2941 ccgctggtcc gattgaacgc gcggattctt tatcactgat aagttggtgg acatattatg
     3001 tttatcagtg ataaagtgtc aagcatgaca aagttgcagc cgaatacagt gatccgtgcc
     3061 gccctggacc tgttgaacga ggtcggcgta gacggtctga cgacacgcaa actggcggaa
     3121 cggttggggg ttcagcagcc ggcgctttac tggcacttca ggaacaagcg ggcgctgctc
     3181 gacgcactgg ccgaagccat gctggcggag aatcatacgc attcggtgcc gagagccgac
     3241 gacgactggc gctcatttct gatcgggaat gcccgcagct tcaggcaggc gctgctcgcc
     3301 taccgcgatg gcgcgcgcat ccatgccggc acgcgaccgg gcgcaccgca gatggaaacg
     3361 gccgacgcgc agcttcgctt cctctgcgag gcgggttttt cggccgggga cgccgtcaat
     3421 gcgctgatga caatcagcta cttcactgtt ggggccgtgc ttgaggagca ggccggcgac
     3481 agcgatgccg gcgagcgcgg cggcaccgtt gaacaggctc cgctctcgcc gctgttgcgg
     3541 gccgcgatag acgccttcga cgaagccggt ccggacgcag cgttcgagca gggactcgcg
     3601 gtgattgtcg atggattggc gaaaaggagg ctcgttgtca ggaacgttga aggaccgaga
     3661 aagggtgacg attgatcagg accgctgccg gagcgcaacc cactcactac agcagagcca
     3721 tgtagacaac atcccctccc cctttccacc gcgtcagacg cccgtagcag cccgctacgg
     3781 gctttttcat gccctgccct agcgtccaag cctcacggcc gcgctcggcc
//
LOCUS       DAJVUJ010000245         3817 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.247, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000245 DAJVUJ010000000
VERSION     DAJVUJ010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3817
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.247"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            1..211
                     /locus_tag="OQQ48_004056"
                     /pseudo
     CDS             1..211
                     /locus_tag="OQQ48_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310610.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
     gene            193..1575
                     /gene="torZ"
                     /locus_tag="OQQ48_004057"
                     /pseudo
     CDS             193..1575
                     /gene="torZ"
                     /locus_tag="OQQ48_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
     gene            complement(1740..2711)
                     /gene="cmoB"
                     /locus_tag="OQQ48_004058"
     CDS             complement(1740..2711)
                     /gene="cmoB"
                     /locus_tag="OQQ48_004058"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HCS1954623.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRVVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(2708..3451)
                     /gene="cmoA"
                     /locus_tag="OQQ48_004059"
     CDS             complement(2708..3451)
                     /gene="cmoA"
                     /locus_tag="OQQ48_004059"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HCS1954624.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MISMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            complement(3492..>3817)
                     /locus_tag="OQQ48_004060"
     CDS             complement(3492..>3817)
                     /locus_tag="OQQ48_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HCS1954625.1"
                     /translation="RLQYRVAYGDGGFSELQSAIRIHGNAVEYIPIAIVLMLFMEMNG
                     AETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRSGMSATWCALLLMVLANLWYMPWEL
                     VFSLR"
BASE COUNT         1009 a          950 c         1009 g          849 t
ORIGIN      
        1 aggcgagctg gctaccgtga atcctggtac cgatctcacc gtcgttgatg ccagtggcaa
       61 acagccgatc gttctgttgc agggttatca aatgcagggc agtgaaaaca cgctctacct
      121 ggcggcaggt caacggctgg cgctagccac attaagtgaa gaaggtatca aggcgctcac
      181 gctaaacggg gaatggcagg ctggggaatt cagcgccagc aatatggtga acagaaacac
      241 tggatgctgg tgacgctggc ggcggtgtta gggcagattg gtacaccggg cggcggtttt
      301 ggtttctctt atcattactc caatggtggc aacccgacac gtgttggcgg cgtgctgccg
      361 gaaatgtctg cggcgattgc cggacaggcc agcgaggccg ctgatgatgg cggaatgagg
      421 gcgattcctg ttgcccgtat cgtcgatgcg ctggaaaatc ctggcggcaa atatcagcat
      481 aacggtaaag agcaaacgta tccgaatatc aaaatgatct ggtgggcggg tggcggtaac
      541 tttacccatc accaggatac taatcgtctg attaaagtat ggcagaaacc ggagatgatc
      601 gtcgtttctg aatgctactg gaccgcagct gctaaacatg cagatatcgt attaccgatc
      661 accacatcgt ttgagcgcaa tgacttgacg atgaccggtg attacagcaa ccagcatatt
      721 gtgccgatga agcaggctgt cgctccgcaa tttgaagcgc gaaacgattt tgatgtgttt
      781 gccgatcttg cggaattact gaaacctggc ggaaaagaga tctataccga aggtaaagat
      841 gaaatggcgt ggctgaaatt tttctatgat gccgctcaga aaggtgcccg tgcgcaacgc
      901 gtgactatgc cgatgtttaa tgccttctgg cagcaaaata aactgatcga aatgcgccgc
      961 agcgagaaga acgaacagta cgttcgttat gctgatttcc gcgccgatcc ggtgaaaaat
     1021 gcgctgggta cgccaagcgg caaaattgag atttactcca aaacgctgga aaaatttggc
     1081 tataaggatt gcccggcaca tccaacctgg cttgcgcctg atgagtggaa gggtaccgcc
     1141 gacgagaagc agttgcagct tctgaccgca catccggcac accgtttaca tagtcagctt
     1201 aactatgcgg aactgcgtaa aaaatatgcg gttgcagatc gtgaaccaat cactattcat
     1261 accgaagatg ctgctcgctt tggtattgcg aatggtgatc tggtgcgtgt gtggaacaaa
     1321 cgtggtcaga ttctgacagg cgcggtggtg actgacggga tcaaaaaagg cgtggtatgc
     1381 gtgcatgaag gtgcatggcc agatctggaa aatggcttgt gtaaaaacgg cagtgcgaac
     1441 gtgttaacgg cggatatccc cagctcgcag ctggcaaatg cctgtgccgg taactctgcg
     1501 ctggtgtata tcgaaaaata tacgggtaac gcaccgaagt taacggcatt tgataagcct
     1561 gctgttcagg cgtaagaagt gaatagcgcc cggaaacggg cgctgaggtt atgcaaacgt
     1621 aaaactgcct gatgcgctat gcttatcagg tctacatagt ccctgcaata gattgaatta
     1681 gcaagggttt gtaggccgga taaggcattc acgctgcatc cggcagtaat accagacctt
     1741 tacggcttgc gcgcaatcag caccgcgcgt ttaggcgcag gataaccttc aactgtctta
     1801 ctcggatcat gtgggtcgag gaaatcggac agagattcgg taaccatcca ttcagtgcgt
     1861 cgctgctctt cagtagtggt aacgcacaca tccacaacgc ggatatctac aaaaccacac
     1921 ttcttcagcc agtttttcag cgccagcgcg gaagggatga aatagacatt acgcatttgc
     1981 gcgtaacgat cgcctggtac cagcaccgtg ttctcatcgc catcaataac cagcgtttcc
     2041 agcaccagtt caccctcatt caccagttga tctttcaact gccagagatg ctccagcggc
     2101 gaacggcgat ggtaaagcac gcccatcgaa aagacggtat caaaggcttt cagtgccgga
     2161 agctgttcaa tacctaacgg taacaaatgt gcgcgctgat cgttacccag tagtttacgc
     2221 accgcttcaa actggcacag aaacagttgc gtagggtcga tccccaccgc gaggtgcgcg
     2281 cctgcgccaa tcatgcgcca catgtgataa ccgctgccgc agccgacatc cagaatggtg
     2341 cgtccggtta aatcagaaag atggggcaga acacgatccc atttccagtc ggaacgccat
     2401 tcggtatcga tgttgacgcc atacagtgag aacggccctt tgcgccatgg catcaggttc
     2461 cgcataagcg tttcaatgcg tttaatttgc ccggtgctca gtggctcttc gctttcggcg
     2521 gttacgctat gcaataaatc cagacgatac ggtttaattt caggcagaaa ttccaccgcg
     2581 ttggaccact gcttaaacag cccgtgctgc tgctcgcgct gccagttagc aatctgcgcg
     2641 ggcagcgttt cgagccagtg tgaaagatga tttttggcaa tcagagaata aaagttacca
     2701 aagtcgatca tgcagcgtcc tctgctttta atgccaccag tgaaccaaag ttaaagcact
     2761 ggaaccacag ctcgctatgc tcaaaaccgg ctttatgcag gcgtgcttta tgggtttcca
     2821 cggaatcggt cagcatcacg ttttccagca tgctgcgctt ctggctgatc tccagttcgc
     2881 tgtaaccgtt ggcacgttta aagtcgtggt gcatgttgaa caacagttca ccaactttgg
     2941 catcttcgaa actgaatttt tccgaaagca ccagcgcgcc gcccgggttc agtccttgat
     3001 aaattttatc cagtaatgcc tggcgctcgg aaggttccag gaattgcagg gtaaaattca
     3061 gcaccaccat cgatgcgttt tcaatggcga tatcgcgaat atcaccttca atgacgtcta
     3121 ctggcgtagg ggctttatag gcgtcaatat gacgacggca gcgttcaatc atcgccgggg
     3181 agttgtcgat ggcaataatt ttgcaattat catgatgaat gttgcgacgc accgagagcg
     3241 tcgccgcgcc cagagaacaa cccagatcgt aaacctgcgt accaggttga acgaagcgct
     3301 cggctaacat actaatcatg gaaataatat tggaatagcc gggaacggaa cgctggatca
     3361 tatccgggaa gacttcagct acccgttcat caaaggtcca gtcgcccagt ctggcgatag
     3421 gggcagaaaa tagcgtgtcg cggtgagaca taacgtaaaa atccgggaaa aagaaagtgg
     3481 cgtattgtgc gctaacgcag ggagaaaacc aactcccagg gcatatacca aagattcgcc
     3541 agcaccatca gcaacagcgc acaccaggtg gcgctcatgc cggaacgtcg ccagcgaaac
     3601 agacggtgat gaaaaccgta ataatgcatc agacgaccag caagcaaaac gatgccgcaa
     3661 atatgcacca tccaggtttc tgcgccattc atttccataa acagcatcag cacaatcgcg
     3721 atgggaatat attccaccgc gttaccatga atgcgaatag cgctttgcag ttcgctaaaa
     3781 ccaccgtcac cataggcaac gcggtactgc aaacgca
//
LOCUS       DAJVUJ010000246         3737 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.248, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000246 DAJVUJ010000000
VERSION     DAJVUJ010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3737
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.248"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(<1..860)
                     /locus_tag="OQQ48_004061"
     CDS             complement(<1..860)
                     /locus_tag="OQQ48_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HCS1954626.1"
                     /translation="MSSHFTPFTLKDISRPGGGFAMLAVDQREAMRLMFAAAGQPKPI
                     ADSVLTDFKVAATRILSPYASAVLADKQFCLEQIVEQGAVANSCGLIVAADLFIPGNG
                     IPVDSVEIDMSVDPHKAREMGAKAMKLLVLWREDEPAEERLAMVDKFVRRCRSAGLVS
                     IIEPVVRPPRRGWDFDRESAIVAAAAELGGTEADLYKAEMPLGGKGDEKTLLAACQQL
                     NDQMKMPWVILSSGVDADIFGRAVSIAMKGGASGFLAGRAVWASVVGAQDPQTMLRDV
                     SVPRLQRLAE"
     gene            complement(885..1763)
                     /gene="yihU"
                     /locus_tag="OQQ48_004062"
     CDS             complement(885..1763)
                     /gene="yihU"
                     /locus_tag="OQQ48_004062"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HCS1954627.1"
                     /translation="MATIAFLGLGQMGSPMANNLLQKGHSLQVFDVNAQAVDALVTQG
                     ATAAQTQAEAAAGAEFIITMLPNGDIVRQVLLGEKGVCEAVSSDALLIDMSTIHPLQT
                     DALIRELQEKGISMMDAPVGRTSVNAIDGTLLILAGGTDEQIARARPILMCMGNELVE
                     AGGPGMGIRVKLINNYMSIALNALSSEAAVLCESLGLNLDVAIKVMSGTAAGKGHFTT
                     TWPGKVLKGDLSPAFMVDLALKDLRIAVDVARETGTPLNMGIAAESYYAAASQDGKGR
                     QDWSALLNQVRQQAGL"
     gene            1936..2832
                     /locus_tag="OQQ48_004063"
     CDS             1936..2832
                     /locus_tag="OQQ48_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCS1954628.1"
                     /translation="MTRIACVGITVQDRIYSLPTLPEGGGKYQANHYLEIGGGPAATA
                     AVAIAKLGVEVDFIGRVGDDSCGNTLLAELEGWGVNTAFCRRYPNARSSQSAILVDQH
                     GERIIVNYPSPDLGTDAEWLEAIDFSRYNLILADVRWHSGTEKAFSLARLAGVTTLLD
                     ADMTPQDISPLVALADHAVFSTPGLKRMTGLQSPEEGLFQATTQTAGKVYVTLGSEGS
                     LWIEDGHLCQQEAFSVNVVDTTGAGDVFHGALAVALAEKMPTKEAIRFASAVAAMKCT
                     QPGGRAGIPNREQTESFLSLYA"
     gene            2872..3660
                     /locus_tag="OQQ48_004064"
     CDS             2872..3660
                     /locus_tag="OQQ48_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCS1954629.1"
                     /translation="MSIIEVTGNPRHDQLVHLIAERGYMNIEELAQLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRVSSVMNTAFEQRELSLTAEKRAIAEAVADYLPERCTVFI
                     TIGTTVEAVARALLNRRDLRIITNSLRVAQILYKNQDIEVMVPGGTLRAHNGGIIGPG
                     AVDFIEGFRADYLITSIGAIEHDGTLLEFDLNEALVARTMIKHARNTLLVADHTKFAA
                     SAAVSIGNARNVRAFFTDAPPPNSFCQLLSEENVELVVAEQEVS"
BASE COUNT          962 a          955 c          902 g          918 t
ORIGIN      
        1 atttctgcca accgttgtaa acgaggaaca gagacatcgc gcagcatagt ttgtggatct
       61 tgtgcaccaa caaccgacgc ccaaaccgct cgtccagcca ggaagcccga cgcaccacct
      121 ttcatggcaa tacttactgc acggccaaaa atatcggcat caacgccaga agaaagaatt
      181 acccacggca tcttcatctg atcgttaagt tgctgacagg cggcaagtaa ggttttttca
      241 tcgcctttgc cccctaatgg catctcggct ttgtaaagat cagcctctgt tccaccaagc
      301 tctgcggcgg cggcaacaat ggcgctttca cgatcaaagt cccaaccgcg acgaggtgga
      361 cgcaccacag gttcaataat gctcaccagc ccggcactac ggcagcgacg aacaaattta
      421 tcaaccattg ccagacgttc ctctgcgggt tcatcttcac gccagagtac aagtaatttc
      481 attgccttag cccccatttc acgggcttta tgcgggtcga cggacatatc tatttcaacg
      541 ctatcaaccg ggatgccatt acctgggata aaaagatcgg cagcgacaat caggccgcaa
      601 gaattagcaa cagcgccctg ctcgactatt tgctcaagac aaaattgttt atccgccaga
      661 acggcagaag cataaggaga aaggattcgg gtcgcggcaa ctttaaaatc ggtgagtaca
      721 ctgtcggcga tgggttttgg ctgccctgct gccgcaaaca tcaagcgcat ggcctcacgc
      781 tgatcgacag ctaacatggc aaatccacca cctgggcggg agatatcttt cagcgtaaat
      841 ggggtgaagt gggatgacat agaaactcct gttaatagat aaaattaaag accagcctgc
      901 tggcgaacct gatttagcag ggcactccag tcctgccgcc ctttgccgtc ttgacttgct
      961 gcagcgtaat aactttctgc tgcaatcccc atattgagcg gtgtccccgt ctcccgagcg
     1021 acgtcaactg caatacgcag atctttcaac gccagatcca ccatgaaggc gggtgaaaga
     1081 tcgcctttca gcactttgcc aggccaggtg gtcgtaaaat gtcctttccc tgctgccgta
     1141 ccactcatca ccttaattgc gacatcaaga tttaatccca gtgactcaca taacacagcg
     1201 gcctcggagg acagcgcatt aagcgcaatg ctcatatagt tgttaattag cttcacgcgg
     1261 atccccattc ctgggccgcc agcctcgacc agctcattac ccatgcacat aagaatcggg
     1321 cgtgcacgtg caatctgctc atcagtgcca cccgctaaaa tcagcaatgt gccatcaatc
     1381 gcattcacgg acgttctgcc cacgggagcg tccatcatac tgatgccttt ttcttgtaac
     1441 tcccgtatta atgcatcggt ctgtaaggga tgaatagtcg acatatcaat caataacgcg
     1501 tcagatgaaa cagcctcaca gacgcctttt tcccccaaca gaacctgtcg aacaatgtca
     1561 ccattgggaa gcatagtgat gataaattca gcgcctgctg ctgcttccgc ttgggtttgt
     1621 gccgcagtgg ccccctgcgt aactagcgca tcgacggcct gtgcattgac atcaaatacc
     1681 tgcaatgaat gccctttttg caatagattg ttagccattg ggctacccat ctgccccagc
     1741 ccgagaaatg caatggttgc catgaatact ctcctgtctg acattgacaa aataatgaca
     1801 attgaaaatt cgtttttgtc tgtttttgag catatttgtg atttatgttc aaaaacacga
     1861 cacgcatcac taatttgatc atttgcgtca tttaaattga ccaaagacga caaaattaaa
     1921 caggacaaga caaatatgac cagaattgca tgcgtgggta tcacagttca ggacagaatt
     1981 tattctttgc ccactctgcc ggagggcggc gggaaatatc aggcaaatca ttatcttgag
     2041 attggtggcg gcccggcagc aacagccgct gttgcaatcg caaagcttgg cgtggaggtt
     2101 gatttcatcg gtcgcgtcgg cgatgacagt tgtggaaata ccttacttgc agaacttgag
     2161 ggttggggcg tcaataccgc attttgccgc cgatatccta acgcccgttc gtcgcaatca
     2221 gcgatcctcg ttgatcagca tggcgaacgg attatcgtta attatccaag tcctgatctc
     2281 ggtactgatg ccgaatggtt agaagcgatc gatttctctc gttataatct gattctggcc
     2341 gatgttcgct ggcatagcgg tactgaaaaa gcattctcgc tagcgcgcct tgcgggtgtg
     2401 acaacgcttt tagacgccga tatgacaccg caggatattt ctccacttgt cgcgcttgcc
     2461 gatcacgccg tgttctcaac accagggctg aaacgcatga ctggcctgca gtctcctgaa
     2521 gaaggtttgt tccaggcaac gacacaaaca gcaggtaaag tgtatgttac gctgggttct
     2581 gaaggatcgt tgtggattga agatggtcat ttgtgtcaac aggaggcatt ttctgtcaat
     2641 gtggttgata ccactggagc aggcgatgtt tttcacggcg cactcgcggt agcgttggca
     2701 gaaaaaatgc caacaaaaga ggctattcgt tttgccagtg cggttgccgc gatgaaatgt
     2761 acccagcctg gcggtcgggc cggcattcct aatcgtgagc aaaccgaatc atttttgtca
     2821 ctttatgcgt aaaatggtaa acatctaccg aggaggggca agggagtatt catgagtatc
     2881 atcgaagtga caggtaatcc acgccatgac caactcgttc atctaatcgc agaacgcggt
     2941 tatatgaata ttgaagaact ggcacaactg ctggatgttt caacgcaaac cgtgcgccgg
     3001 gatatccgaa aactcagtga gcagggcttg atcacccgtc atcatggagg ggccgggcgc
     3061 gtctccagcg tcatgaatac cgcttttgag caacgggaac tttcgctcac cgccgagaaa
     3121 cgggcgatcg ctgaggcagt cgccgattac cttcccgaac gctgtaccgt ctttatcacc
     3181 atcggtacaa ccgtagaagc cgttgccagg gcattactca accggcgtga tttacgcatt
     3241 atcactaaca gcctacgtgt ggcacagatt ctttataaga atcaggatat tgaagtgatg
     3301 gtgccgggag gcactttacg cgctcataac ggcgggatta tcggcccagg agccgtggat
     3361 tttattgaag gcttccgcgc agattattta atcaccagta taggtgccat tgaacatgac
     3421 ggcaccctac tggaatttga tctaaatgaa gcgttagtcg cgagaacgat gattaaacat
     3481 gcgcggaata cattgttagt ggccgatcat acgaagtttg ccgcgtctgc tgccgtttca
     3541 attggcaatg cacggaatgt cagggctttt tttactgatg ccccgcctcc caattctttc
     3601 tgccagttgt taagtgaaga gaatgttgaa ctggtggttg ccgagcaaga agtatcctga
     3661 aattgaaatt tcggaaaatc cgctgggaat ttcagcggat tttgttttcc ataagcaaaa
     3721 aaaacctgcc agcgatg
//
LOCUS       DAJVUJ010000247         3725 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.249, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000247 DAJVUJ010000000
VERSION     DAJVUJ010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3725
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.249"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            37..264
                     /locus_tag="OQQ48_004065"
     CDS             37..264
                     /locus_tag="OQQ48_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HCS1954630.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
     gene            complement(265..639)
                     /locus_tag="OQQ48_004066"
     CDS             complement(265..639)
                     /locus_tag="OQQ48_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HCS1954631.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(722..1946)
                     /gene="mdtG"
                     /locus_tag="OQQ48_004067"
                     /pseudo
     CDS             complement(722..1946)
                     /gene="mdtG"
                     /locus_tag="OQQ48_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
     gene            complement(2118..3038)
                     /gene="lpxL"
                     /locus_tag="OQQ48_004068"
     CDS             complement(2118..3038)
                     /gene="lpxL"
                     /locus_tag="OQQ48_004068"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HCS1954632.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            3263..3725
                     /locus_tag="OQQ48_004069"
                     /pseudo
     CDS             3263..3725
                     /locus_tag="OQQ48_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415573.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
BASE COUNT          915 a          951 c          946 g          913 t
ORIGIN      
        1 cgttatgccg gtattttttt acggaagagg tatcaaatgc gtttaattgt ggtgagcatc
       61 atggtgacct tgctgagtgg ctgtggcagc attattagtc gcactatacc ggggcagggg
      121 catggcaacc aatattatcc cggtgtgcaa tgggatgtgc gtgactccgc ctggcgttat
      181 gtcacaatcc ttgatctgcc attctccctg gtttttgata ctttactgct gccgatcgac
      241 attcatcatg gcccgtatga gtgattaacg ttcatcccac tcatcagctg ctgcgcgtcc
      301 ttcctcggta tccagcggtg gctcaagctg aaattccccc tcgtcccatt catgtaatgt
      361 attctcttcc tgccactcct ggcgtatctc tatctcatca tagtcgccat caaaaacact
      421 ttgcgcggct tcaccgctaa gcataggtaa acattcacct tcttcccctt cgtcggcaaa
      481 aaactcaact tgccacatga tgtcgccgtc ctgcaaaacg tatttttggg cattgaactg
      541 ttgcacattc gcatcttcgg cgtcgatgcc ggggttgtct gcaagaaatt cttcgcgtgc
      601 agcgtcaatg gcttcttcaa gcgttgcgta catggtcatc acgattctcc ctttgagttg
      661 atgaggtttc agggaaaagg atagctgatt ctccgctttt gcaagtatga aaggcgaaaa
      721 atcagttcga tacctggggt attcgacgac gacgtagact gttccatgaa tagactgcgt
      781 tgaataacac tacgccagcg gtgacgagaa ataccgctct gaaaccgtag ttcgctgaaa
      841 tcgctgctcc catcaatggt ccggtaacgt tgccaatatc acgaaacgat tggttatagc
      901 tgaagatacg cccggcgatc tggttgctcg agttgtaaac caacaatgtc tgtacggcgg
      961 ggagtagtgc accatcggcg gcaccgagca aaaaacgtaa aatcccaagt tgcaatggcg
     1021 tctgaacgta agacattggg atcaacagca gtacagaaaa gatcagcgct gtaatcagga
     1081 tcttttcggg tccgattcga tcgccaagtt tgccgagtcg tggtgcactt agcagagccg
     1141 ccacgcctgg caccgaggcg atcatgccac tgataaaggc gacgttactg acgttacccg
     1201 ccagttcgcg gacatacagc gtcagaatgg gggcaattga gcccgtcgcc acctggatga
     1261 ttaacgtagt gacaaacagg ctgagtacca gtttcgggtt tttaagtgat gtcaccactt
     1321 cccgcatgtg cagcatctct tttttgctga ccggctggaa tttttctctg atgcaaaaca
     1381 gggtgacgaa aaagcagagt atgagcacac tggtggtaat aaagaatacc ggacgtaagc
     1441 cgtagctatc ggcgagcagg ccgccagcca ttgggccgag caacgcacca ccaacgccgc
     1501 ctgtggagag cgtacccagc gcccagccgc ttttattacg cggtacttgt gtggcgataa
     1561 gagcattagc gttggggaca aatccgccaa gtaacccaag aagcgcccgc aggatcaaaa
     1621 actgccagat attttgtgcc agccccatca acaccatcac gatgcccatg ccgagggcag
     1681 agcgtaatag catgagtttt cggcctttac ggtcggcgag tccaccccaa aacggtgagg
     1741 tgatggccga aaataaaaat gtaatgctga agacaatacc ggaccatatt cagggcggag
     1801 tgaccggtaa cgccaagctg ctcaacgtag aggggtaaga agggcattac cagactgaag
     1861 gcggcaccgg taagaaaaca gcctagccag gcgacgatca ggtttcgttt ccagtttata
     1921 ggggtgtcat tttcacaggg tgacatagca atccgctgtt ggtgcgccag gcgcggtgaa
     1981 cataagaaga aaagataagc acactaatta tgcgcccgac ttccaggggg cgcaatccag
     2041 agagctttta tcgctaaatc agggggattt gctgtggtaa tgccggatgc cattctgaag
     2101 catccggcag gggagattta atagcgtgaa ggaacgcctt ccgggcgtgt tttaaagcga
     2161 cggtgtaacc acatatactg ctctggtgcc atcatgatgc atttttcgac cactttgttc
     2221 atccacgcgg cggtagtttc ggcatcatcc agtggcggag aacactctgg cggcagcata
     2281 atcaattgat accctttgcc atctggctta cggcgtggaa cgaagggcac cagacatgcg
     2341 ccggacatcc gtgccagcat ccaggttccg gtcgtggtcg cagcctgctc aacggcaaat
     2401 aacgggacga aaacgcttga gcgcgggccg taatcatgat ccggtgcgta ccagaccact
     2461 tcgccttttt tcagggcttt aatcatgcct tttaaatctt tgcggtcgag catcgattta
     2521 tttgagcgca aacggcccca ggtttgtagc cagtcaatca gtggattatc gttcgggcgg
     2581 taaacgccaa tccccggttc ctgcatacca aactgccgcg cacccagctc cagtgtcaga
     2641 aaatggatgc caactaacag gatgccgcgt ttttgcgcct gcacgtcacg aatgtgttcc
     2701 atgccgatca cttccgtcca gcgcgcgatt cggcggtccg gccagaacca cgccatgccg
     2761 gtttccatca ggcccatgcc aacggattcg aaattcttca ccaccatttt acggcgttct
     2821 tgttcgctca tttccgggaa gcacagttcc aggttgcgat gcacaatttt tgcgcgtcgt
     2881 ttcataaaac gtaacgccag ttttcctaat ccacaaccga ggcggtagat aaccgggtag
     2941 ggcaattgca cgactaacca aagtacgcca atacccaacc aggttaacca ataacgcgga
     3001 tgaagcagtg cggtggagaa cttgggtaga ttcgtcatat caatcctgtt tttcaaccta
     3061 ttcgggcaat tgtatgtatt gtcgcatttt ttcgcccgca accaaaattt gtggctgaag
     3121 actgggcgaa attgccgcgc ttgtaaataa caaataattt ttaatgcgca aatgtagcgt
     3181 aaaatgtgtg gatgttaatt atcgataatt gctatatcat gccgcggatt tttactttcc
     3241 catctcgcag gaaccgtaca ccatgccagt gttacacaac cgcatttcca acgacgcgct
     3301 aaaagccaaa atgttggctg agagcgaacc gcgaaccacc atttcgtttt acaagtattt
     3361 ccacatcgcc gatcctaagg cgacccgtga cgctttatat cagctgttta ccgcgctgaa
     3421 tgtttttggg cgagtgtatc tggcgcatga gggcattaac gcgcaaatca gcgtacctgc
     3481 gagcaatgtt gaaacatttc gcgcgcagct ctgccttcga cccggcttta gagggtttac
     3541 gcctgaatat cgcgttggat gatgacggga aatccttctg ggtactgcgc atgaaggtac
     3601 gtgatcgcat tgttgccgac ggtattgacg atcctcactt tgatgccagc aatgttggtg
     3661 agtatctgca agcggcggaa gtgaacgcca tgcttgacga tcccgatgca ctatttatcg
     3721 acatg
//
LOCUS       DAJVUJ010000248         3704 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.250, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000248 DAJVUJ010000000
VERSION     DAJVUJ010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3704
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.250"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            115..618
                     /gene="yeaK"
                     /locus_tag="OQQ48_004070"
     CDS             115..618
                     /gene="yeaK"
                     /locus_tag="OQQ48_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HCS1954633.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTTDYLRIAQPEL
                     VNFRRTA"
     gene            complement(619..723)
                     /locus_tag="OQQ48_004071"
     CDS             complement(619..723)
                     /locus_tag="OQQ48_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1954634.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            892..1338
                     /locus_tag="OQQ48_004072"
     CDS             892..1338
                     /locus_tag="OQQ48_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HCS1954635.1"
                     /translation="MVDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(1295..2116)
                     /gene="nimR"
                     /locus_tag="OQQ48_004073"
     CDS             complement(1295..2116)
                     /gene="nimR"
                     /locus_tag="OQQ48_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HCS1954636.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            2213..3409
                     /gene="nimT"
                     /locus_tag="OQQ48_004074"
     CDS             2213..3409
                     /gene="nimT"
                     /locus_tag="OQQ48_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005117270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HCS1954637.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGSNSLVYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGNWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     RSYPVL"
     gene            3449..>3704
                     /locus_tag="OQQ48_004075"
     CDS             3449..>3704
                     /locus_tag="OQQ48_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HCS1954638.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFTTFREQYLAELAQHEQEGKRLADI"
BASE COUNT          848 a          933 c          936 g          987 t
ORIGIN      
        1 ttacatgatg cgtataaagc ttccgatgag cgtttatatg tcaataagca gaacaaaaac
       61 agccgttcat gataaccttc tgtggttgtt tgcttgtaat ctcaggagcg tgaaatgact
      121 gaaatggcta aaggaagcgt gacgcatcag cggttaatcg ctttattatc acaagaaggt
      181 gctaacttcc gcgtcgttac ccatgaagcg gtaggtaaat gtgaagcggt gtcagaaatt
      241 cgtggcaccg ctttaggcca gggagctaag gcactggtat gtaaagtcaa aggcaatggc
      301 gtaaatcaac atgttctggc gatcctcgct gccgatcaac aggccgatct gagccaactt
      361 gccagccata taggcggatt acgcgcttcg ttagccagcc ctgctgaagt cgatgaactc
      421 acaggctgtg tcttcggcgc gatcccccct ttcagcttcc atccaaaact caaactggtt
      481 gccgaccctc tactcttcga acgatttgat gaaatcgcct tcaatgcagg gatgctggat
      541 aaatccgtta ttttgaaaac cacggattat ttgcgcattg cgcaaccaga actggtcaat
      601 ttccgccgca ctgcgtaact agcctgtccg ttcaataagc agaacggaca aaaccagcac
      661 aacagcaatg gcaaaaaacg atgacgtgat aatcagtgtc tcgacgaaca tttgatcgtt
      721 catagcatgc ccccgggaga tagctgtttt cacgttattg ttagcgtgca caaatggcag
      781 tttgatgaca gttcgcctga tttttatgca aaaaacgtaa agattttcta cttccttcct
      841 gcagcaagcg taaagtaagc aggcttatta ttttttggca aggaaaccac gatggttgat
      901 gtcactttgc tgatcctgct cgggttagct gcgctgggct ttatcagtca taacaccact
      961 gtcgccgttt caattctggt gttgatcatt gtccgcgtca caccgttaag cacctttttc
     1021 ccgtggattg aaaaacaggg gctaagtatc gggataatca ttctgacgat tggcgttatg
     1081 gcccccatcg ccagcgggac gctaccgccc tcaaccttga ttcactcgtt tctgaactgg
     1141 aaatcgctgg tggcaattgc agtaggcgtg attgtctcct ggctgggtgg gcgcggcgtg
     1201 accttaatgg gcagccagcc gcaactggtc gccgggctgc tggtaggcac tgttttaggc
     1261 gtggcgctgt ttcgcggcgt accggtcggc ccgcttattg ccgcgggtct ggtttcgctg
     1321 attgtgggga aacagtagtt aatcccgcaa tatatcggcc tggcgtttgc cccaggcctt
     1381 tcttaaacat agtaataaag gccgttgttg aatcataccc cagcgtatga gcaactttct
     1441 gtacagtatc gcctttcact aatccctgaa gtgccataat cagctgtaac tgctgacgcc
     1501 actggcgaaa actcaacccc gtctctttta cgatcagtcg cgcaagattg cgttcactca
     1561 tcgcaaaaaa gccagcccat tgccctaatg acccccactc gacgggccct ttcgccatca
     1621 tctccaccat cgtgcggatt ttaggatgag aagaaacggg caggtgcagc tgttgttgtg
     1681 gctgctgagg gagttcatca aacaggacct gtattaatct gcgggtcatc ggctcagcac
     1741 gctgagtcgt cgtcctgttt gccagggtta aaatcaattc ccggcataag ggcgatattt
     1801 tcaatgtgca acatgttgtc ggcattgtca ctgcgctggg ttcgataaac agaaaacaga
     1861 gttcagcgtt agctgtcact tgattgctat gttccacccc gcccggtatc caaaccgcat
     1921 attgcggcgg caccatccac aaagcatttt ccaccgtaca ggtaattgca ccatgtaatg
     1981 ctagtatcag ctgtcctttg cggtgctgat gtacaggact ggttaactca tccgttccgg
     2041 cgtgaataca aaatgcgacc gcggcttcat ggtgtcggtc gggttcatag ccattgagat
     2101 tcaacctgtg catcattttg tccgaattta gcgataattt gtcattttag cttgattcaa
     2161 cataacaata aaaacggtaa ggtacagcct cgtttgtaac aatgagaagc atatgacctg
     2221 ttcaacttca ttaagcggca aaaacaggat tgtccttatc gctggcattc tgatgattgc
     2281 cacaacatta cgcgtcacct ttaccggcgc agcaccgtta ctggatacga ttcgttccgc
     2341 ttactcgctg acgacagcgc aaaccggctt attgaccacc ctgccattat tggcctttgc
     2401 gctaatctca cctttggccg ccccggtagc gcgacgtttt ggtatggaac gtagcctgtt
     2461 tgccgcgtta cttttgatct gtgctggtat cgcaattcgc tctctccctt cgccttactt
     2521 attatttggc ggtacagcgg tcattggcgg tgggattgca ttaggcaatg tcttactgcc
     2581 aggattaatt aaacgcgatt tccctcattc cgtcgccaga cttaccggcg catattccct
     2641 gacaatggga gctgcggcgg cacttggatc ggctatggta gtgccgctgg ctttgaacgg
     2701 ttttggctgg caaggcgcgt tgctcatgct gatgtgtttt cctctgctgg ctcttttttt
     2761 atggctgcca cagtggcgaa gccaacaaca tgcaaatttg agtacctcgc gcgccttaca
     2821 tactcggggt atctggcgtt cgccgcttgc ctggcaggtc acattgtttc ttgggagcaa
     2881 ctcactggtc tattacgtga ttattggctg gcttccggcg atcctcatca gtcacggcta
     2941 tagcgaagca caggcgggtt cactgcatgg tttgctgcaa ctagccacag cagctcccgg
     3001 tttgctgatc cctcttttct tacatcatgt gaaagaccag cgtggtattg cagcgttcgt
     3061 tgccttgatg tgcgcagtgg gcgctgttgg gctctgcttt atgccagcgc acgcgatcac
     3121 ctggactctg cttttcggtt ttggttccgg cgcaacaatg atactggggt tgacgttcat
     3181 tggtcttcgg gcaagttctg cgcatcaggc ggcggcactc tcggggatgg cacaatccgt
     3241 cgggtatttg ctggcagcct gtgggccgcc gctaatgggt aaaatacacg atgctaacgg
     3301 taactggtcc gtaccactga tgggtgttgc aatactttca ctactgatgg cgattttcgg
     3361 actttgcgcc gggagagaca aagaaattcg ctcatatccg gtgctatagt gacgtgacaa
     3421 atcatgcgtg aaagggagaa caaacacgat gaatattcag tgcaaacgcg tttatgatcc
     3481 ggctgaacag agcgatggtt atcgcgtact ggtcgaccgc ctctggccgc gcggtatcaa
     3541 aaaaaccgat ttagcccttg atgagtggga taaagaaatc acgccgtcaa cggaactgcg
     3601 caaagccttt cacggcgaag tcgtcgattt tacaaccttt cgcgagcaat atcttgcaga
     3661 actggcgcaa cacgagcaag aaggaaagcg gctggcggac atcg
//
LOCUS       DAJVUJ010000249         3686 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.251, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000249 DAJVUJ010000000
VERSION     DAJVUJ010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3686
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.251"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(279..566)
                     /locus_tag="OQQ48_004076"
     CDS             complement(279..566)
                     /locus_tag="OQQ48_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HCS1954639.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            complement(641..784)
                     /gene="mgrB"
                     /locus_tag="OQQ48_004077"
     CDS             complement(641..784)
                     /gene="mgrB"
                     /locus_tag="OQQ48_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HCS1954640.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            943..1182
                     /locus_tag="OQQ48_004078"
     CDS             943..1182
                     /locus_tag="OQQ48_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1954641.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            complement(1325..2116)
                     /gene="kdgR"
                     /locus_tag="OQQ48_004079"
     CDS             complement(1325..2116)
                     /gene="kdgR"
                     /locus_tag="OQQ48_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HCS1954642.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            2293..3666
                     /locus_tag="OQQ48_004080"
     CDS             2293..3666
                     /locus_tag="OQQ48_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1954643.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVMLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRSRA"
BASE COUNT          925 a          904 c          815 g         1042 t
ORIGIN      
        1 taaaataaga aaaattgatg aatgagcaaa aaaatcaaga gagaaacgtt tcgctaataa
       61 ataataccac gcttaatcac aaatccgatg taaacatcct aataaattaa tgaggaaaat
      121 atctttttct gatatcccgt tacgcaaata aataatatga taattatttg aagtcagacc
      181 agagttttaa tttgaagcag ttgcaccatg acagtgcgta taatcaggat gaattgagta
      241 acccttcaac gacggggtta ctcaagattg aggaaggatt atcgttcggc gaccaggcga
      301 ataatatctt cgtcatcaac cgatttttga ctctcaatgg tctttttatc tgctttctct
      361 ggttcgtttg cttcgcacaa acgacgtaac agtgcatttt gacgtttttg ctgatccagc
      421 aacgcctcaa gcagttcaat ctgttcgtta gtccgtgaac tggcacgatt gataaaaaac
      481 cacaagatga gcccgataag aagaactacc accgatacaa ccaaagacgc aatgttcatc
      541 acgcctgaat ttacaacttc gttcatttca ccacctctga gtagaggcgc tattctacca
      601 ctgctggaga ggaagaaaat ctagtgctga aaaaatgata tcaccacggg ataaactggt
      661 taatggcaca aattccgctg aaaaattgta catcctgatc gcacatcatg ttgaatacct
      721 gcgcccaaag cagcaagcaa gccaacacca cgacaaccag aacgacccat cgaaactttt
      781 tcactccaat ctccgcttca cctaccttat gtcattaaac tagcatgcgt tagttaaaca
      841 gcgcctaact atgtcgatat tcgtgctttt tcggaatgag tagcttgctt cagcgcatat
      901 tgccgctacg attaagcgaa cataaaaaga gaagaggttg taatgcgatt catcattcgc
      961 acagttatgc tgatagcact ggtatggata ggattattac tcagcggata tggcgttttg
     1021 attggaagca aagagaatgc cgcaggatta ggtttgcaat gtacctatct gactgccaga
     1081 ggcaccagca cggtacaata cttgcacact aaaagtggtt tcttggggat aacagattgc
     1141 ccactgttac gtaaaagcaa tatcgtggtc gataatggtt aagggtaata aataaccaga
     1201 ttctcactca tgagtaaaat attcttatag caagcgcgtt taatataacg caccttcaac
     1261 tttatttttc tcatgctaaa taaagaacct gtctgtttga tattccgaca ggttcttact
     1321 ctcatcagaa cggatagtcg tgataaccca tttgggcaga aattttgcgc gctgcggtat
     1381 gcaacattgc gacatattcc tgtaaacgct cttcagagaa acgcaacgtc gggaaggaga
     1441 tgctcaaacc ggcaatgacc acgccaaagc gatcaaatac cggtaccgca atgcatcgca
     1501 gcccttcttc ctgctcttcg ttatcttcgc catacccctg ctcgcgcacc tggtccagaa
     1561 cgggtaataa cgcttctgta ctggtgatgg tccgctcggt actgcgttta tactccacgc
     1621 cctcaagaat ttgcttcact tcatcgcgat cgcgccatgc cagcagtacc ttaccaatcg
     1681 cggtgctgta cagcggatta cgacgcccaa tccgtgaata catgcgcaaa ttgtacatag
     1741 agtcaatttt gtgaatgtaa acaatactgt cttcgtccag tgcgccgagg tggatagttt
     1801 ctttggtcag gcgggagatc tcacgcatct ggatatctgc gctacgaatt aaatcgacgt
     1861 tttgtaacgc gcgagcgccc agttcaaaca atttcagggt cagggaatat ttctccgact
     1921 ccccttcctg cgccacataa cctaaggttt tcatggtctg taaaaagcga taaacggtgc
     1981 tttttgacat catgacgcgc tgcgacagct cggttatccc tatttcgcgc tcttcaccca
     2041 gcgcctgcaa aatgccaaaa acttttagca cggaagatac agaatcaggc tgtttatcca
     2101 gatctgcgtt agccatttat cacctcattg cgagtgtttt ataaaaatca gaactgtttt
     2161 ttattataat ttcgcaccag gggggtcgca atccatcttt tgccggttag ttacaattct
     2221 gcgacatcca ccgtgaatat cagtgctaga atcatacccc tgttgattat tcaccaaaga
     2281 tataaaattc ctatgccaaa agttcaggcc gacggcctgc cattgcccca gcgatacggt
     2341 gcgatattaa ccattgtgat tggtatttcg atggctgtcc ttgacggcgc aatcgccaac
     2401 gtcgccctgc caacaatcgc cacggacctt catgccacgc cagccagttc catctgggta
     2461 gtgaacgcct atcaaatcgc cattgtcatc tccctgctct cgttttcgtt tctgggcgat
     2521 atgtttggct atcgacgtat ttataaatgc ggtctggtcg tttttctgtt gtcttcactg
     2581 ttctgcgccc tttctgattc gctgcaaatg ctcacccttg cgcgtgtcat acaaggtttc
     2641 ggcggtgcag cgttgatgag cgttaatacc gcacttatcc gcctgatcta tccacaacgt
     2701 tttctgggga gagggatggg cataaactcg tttattgttg ccgtctcttc tgctgccggg
     2761 ccgacaattg ctgcagcaat cctctccatc gcatcctgga aatggttatt tttaatcaac
     2821 gtaccgttag gtattatcgc cctgcttctg gcgatgcgtt ttctgccacc caatggttct
     2881 cgcgccagta aaccccgttt cgacctgccc agcgccgtga tgaacgcgtt aaccttcggc
     2941 ctgcttatca ctgcgttgag tggtttcgct caggggcaat cgctgacgtt aattgctgcg
     3001 gaactggtgg taatggttgt tgttggtatt ttctttattc gccgccagct ttctcttccc
     3061 gtaccgctgc taccggtgga tttactgcgt atcccgctgt tttcactttc tatttgcaca
     3121 tctgtttgct ctttctgcgc acaaatgctg gcaatggttt ccctgccctt ttacctgcaa
     3181 accgtgctcg ggcgtagtga agtcgaaaca ggtttacttc tgacaccgtg gccgttagca
     3241 acgatggtga tggctccgtt ggcaggctat ttgattgaac gcgtacatgc aggattgctg
     3301 ggtgctctag ggttgttcat catggctgcg gggctttttt ccctggttat gctgcccgcg
     3361 tcacctgcgg atatcaatat tatctggccg atgatcttat gtggcgctgg atttggcttg
     3421 ttccagtcac ccaataacca caccattatt acctccgctc ctcgcgaacg tagcggtgga
     3481 gccagtggca tgttaggaac ggctcgtcta ctgggtcaga gtagcggcgc ggcactggtg
     3541 gcgctgatgc taaatcagtt cggtgataat ggtacgcacg tttcgctgat ggctgcggct
     3601 attctggcag tgattgctgc ctgtgtcagt ggtttacgta tcactcagcc acgatccagg
     3661 gcataaaaaa agcgcgtcga tcagga
//
LOCUS       DAJVUJ010000250         3678 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.252, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000250 DAJVUJ010000000
VERSION     DAJVUJ010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3678
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.252"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(168..482)
                     /gene="fliE"
                     /locus_tag="OQQ48_004081"
     CDS             complement(168..482)
                     /gene="fliE"
                     /locus_tag="OQQ48_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HCS1954644.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQDSLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            697..2355
                     /gene="fliF"
                     /locus_tag="OQQ48_004082"
     CDS             697..2355
                     /gene="fliF"
                     /locus_tag="OQQ48_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HCS1954645.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     LSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTSRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            2348..3343
                     /gene="fliG"
                     /locus_tag="OQQ48_004083"
     CDS             2348..3343
                     /gene="fliG"
                     /locus_tag="OQQ48_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HCS1954646.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTKVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            3336..>3678
                     /gene="fliH"
                     /locus_tag="OQQ48_004084"
     CDS             3336..>3678
                     /gene="fliH"
                     /locus_tag="OQQ48_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="binds to and inhibits the function of flagella
                     specific ATPase FliI; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HCS1954647.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAKAKSQQAPIHARMQQLV
                     SEFQTTLDALDS"
BASE COUNT          926 a          967 c         1028 g          757 t
ORIGIN      
        1 cttcctatga gcaaagcgaa gacaaaacat gttatcgcgt ttacggatcg tggatggcag
       61 aaaactttca ggatcaggta tccattttaa accagaaata gggtgagttt gtcccatcca
      121 tgccccacgc gggcagatgg gatgtaatta ccaacaggtt acaaaaccta cacctgcatg
      181 gacatcactt cctgatacgc cgccaccagc ttattacgca cctgaatccc catttgcata
      241 gaaactgagg ctttttgcat atcggtcatc acatcgttta acgccacgcc gggttcaccg
      301 agggtgaatt tttctgcctg cgtgcgggcg gctgtttgtg tatcgcttat gcgatcgagc
      361 gcggcgtgca gctgcccggc aaaactaatg gtcggttgcg gcagtgaatc ctgcgcacgc
      421 gcactcatcg ccgtagcctg taactggctg ataacccctt caatcccctg tatcgctgac
      481 attttcgtct cctggataat ttctggtagc aaagcctacc agtaagtcaa taagacaaag
      541 gcgctaaata gcaacaaaaa aacgggttta ttggcggata gaaaaaaacg aaagcacaaa
      601 taatggcagc gtcaattttt cgagtttgct gacccgggag tgagtcttgt tccactttgc
      661 caataacgcc gtccataatc agccacgagg tgcgcgatga atgcgactgc agcccagaca
      721 aaatctcttg agtggcttaa tcgcctgcgt gcgaatccga aaattccatt gattgttgcc
      781 ggttccgcgg cagtggcggt catggtcgca ctgatcctgt gggcgaaagc ccccgactac
      841 cgcacattat tcagcaatct ttccgatcag gatggtggcg caattgtcag ccaactgacg
      901 caaatgaata ttccttaccg cttcagcgaa gccagcggcg ctattgaagt tccggcagat
      961 aaagttcacg agctgcgtct gcgcctggcg caacaaggtt tgcccaaagg cggcgcggtc
     1021 ggtttcgaac tactcgatca ggaaaagttt ggtatcagcc agttcagcga acaggtgaat
     1081 tatcagcggg cgctggaagg cgagctttct cgtaccatcg aaactatcgg cccggtaaaa
     1141 ggggcgcgcg tacatctggc aatgccgaaa ccgtctttat tcgtccgtga acaaaaatcc
     1201 ccttctgcat cggtgacggt aaatctatta cccggccgcg cactcgatga agggcaaatt
     1261 agcgccattg tacatctggt ttccagcgcg gttgctggtc tgccgccggg gaacgtcacg
     1321 ctggtggatc agggtggaca tctgttaacc ctgtccaata ccagcgggcg cgatcttaat
     1381 gacgctcagt tgaaatatgc cagcgatgtc gaaggccgga ttcagcggcg tattgaagcg
     1441 atcctgtcgc caattgttgg taacggtaat attcacgccc aggtcacggc gcaactggac
     1501 ttcgccagta aagaacaaac ggaagaacag tatcgcccta acggtgatga atctcatgcg
     1561 gcgcttcgtt cacgccagct taatgagagc gagcaaagcg gttccggtta tccgggcggc
     1621 gtaccggggg cgttgtcgaa tcaaccggca cctgcgaata acgcgccaat cagcacgcct
     1681 ccggcaaatc aaaataaccg ccagcagcag gcgagcacca ccagcaatag cgggccgcgt
     1741 agcacacagc ggaatgaaac cagtaactac gaagtcgatc gcaccattcg tcataccaaa
     1801 atgaacgtgg gcgatgtgca acgtctgtca gtcgcggtag tggtaaatta caaaaccttg
     1861 ccagacggta aaccattgcc tctcagcaac gaacagatga agcaaattga agatctgacc
     1921 cgcgaggcga tgggcttttc tgaaaaacgc ggtgactcgc tcaatgtcgt taactcgccg
     1981 ttcaatagca gtgacgaaag cggcggagaa ctgccgttct ggcaacagca agcgtttatc
     2041 gatcagttgc tggctgccgg tcgctggttg ctggtactgc tggtggcgtg gctgctgtgg
     2101 cggaaagcgg tacgcccgca gttaacaagt cgcgccgagg cgatgaaagc tgtacagcaa
     2161 caggcgcagg cccgtgagga agtggaagat gcggtggaag tccgcctgag caaagacgaa
     2221 caactccaac aacgacgcgc taaccaacgt ctgggggcag aagtcatgag ccagcgtatc
     2281 cgtgaaatgt ctgataacga tccgcgcgtg gtggcgctgg tcattcgcca gtggataaat
     2341 aacgatcatg agtaacctga caggcaccga taaaagcgtc atcctgctga tgaccattgg
     2401 cgaagaccgg gcggcagagg tgttcaagca cctctcccag cgcgaagtgc aaaccctgag
     2461 cgctgcaatg gcgaacgtca cgcagatctc caacaagcag ctaaccgatg tgctggcgga
     2521 gtttgagcaa gaagctgaac agtttgccgc actgaatatc aacgccaacg attatctgcg
     2581 ttcggtattg gtcaaagctc tgggtgagga acgtgccgcc agcctgctgg aagatattct
     2641 cgaaactcgc gataccgcca gcggtattga aacgctcaac tttatggagc cgcagagtgc
     2701 cgccgatctg attcgcgatg agcatccgca aattatcgcc accattctgg tgcatctgaa
     2761 gcgcgcccag gccgccgata ttctggcgtt gttcgatgaa cgtctgcgcc acgacgtgat
     2821 gttgcgtatc gccacctttg gcggcgtgca gccagccgcg ctggcggaac tgaccaaagt
     2881 actgaatggc ttgctcgacg gtcagaatct caagcgcagc aaaatgggcg gcgtgagaac
     2941 ggcagccgaa attatcaacc tgatgaaaac tcagcaggaa gaagccgtta ttaccgccgt
     3001 gcgcgaattc gacggcgagc tggcgcaaaa aattatcgac gagatgttcc tgttcgaaaa
     3061 tctggtggat gtcgacgatc gcagcattca gcgtctgttg caggaagtgg attccgaatc
     3121 gctgttgatc gcgctgaaag gggccgagca gccactgcgc gagaaattcc tgcgcaatat
     3181 gtcgcagcgt gccgccgata ttctgcgcga cgatctcgcc aaccgtggtc cggtgcgtct
     3241 gtcgcaggtg gaaaacgaac agaaagcgat tctgctgatt gtgcgccgcc ttgccgaaac
     3301 tggcgagatg gtaattggca gcggcgagga tacctatgtc tgataatctg ccgtggaaaa
     3361 cctggacgcc ggacgatctc gcgccaccac aggcagagtt tgtgcccatg gtcgagccgg
     3421 aagaaaccat cattgaagag gccgaaccca gccttgagca gcaactggcg caactgcaaa
     3481 tgcaggccca tgagcaaggt tatcaggcgg ggattgccga aggtcgccag caaggtcatg
     3541 agcagggcta tcaggaagga ctggcccagg ggctggagca aggtctggca aaggcgaagt
     3601 ctcaacaagc gccaattcat gcccggatgc agcaactggt cagcgaattt caaactaccc
     3661 ttgatgcgct tgatagtg
//
LOCUS       DAJVUJ010000251         3673 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.253, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000251 DAJVUJ010000000
VERSION     DAJVUJ010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3673
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.253"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(118..2187)
                     /gene="glyS"
                     /locus_tag="OQQ48_004085"
     CDS             complement(118..2187)
                     /gene="glyS"
                     /locus_tag="OQQ48_004085"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HCS1954648.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLVESFAANFTVELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYACHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLDAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2197..3108)
                     /gene="glyQ"
                     /locus_tag="OQQ48_004086"
     CDS             complement(2197..3108)
                     /gene="glyQ"
                     /locus_tag="OQQ48_004086"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HCS1954649.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3203..3502)
                     /locus_tag="OQQ48_004087"
     CDS             complement(3203..3502)
                     /locus_tag="OQQ48_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HCS1954650.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
BASE COUNT          849 a          999 c          945 g          880 t
ORIGIN      
        1 cataaaatta atatatgaga taatctttat tcagcagaag attattaaag gttgctgtat
       61 tatttagcga taataaaaaa agcctgccag atggcaggct ttttaataac ggcgttatta
      121 ctgcaacagc gaaatatccg caacgcgcag gaacagttcg cgcagtttct ccagcatggt
      181 cagacggttg agacgcaatt ctttgtcatc aaccatgacc atcactttat cgaagaaagc
      241 atcaaccggc tcacgcagct ctgccagttc gaccagcgca tcctggtaac gaccttccgc
      301 aaagtacggc tccagcttgt cacgtagcac cacaacctgc atcgccagtt taatttcttc
      361 cggctctttc agggtagagg cattcacgcg gtcgctcagc acttcgtcag atttcgccag
      421 aatgttagat acacgtttgt tcgccgccgc cagtgcagca gctgcatcca gggtacggaa
      481 atgcgatacc gctttcatac gggcatcgaa atcagccgga cgagtcggac gacgcgccag
      541 taccgcctgg atggtgtcaa cggtgtaacc ttcgtcctga taccaggcgc ggaagcgacc
      601 gagcataaag tcgataacat catcaaccac gttggcgtta gtcagcttat cgccatacag
      661 acgcactgct tcttcggtca gcgtttgcag atcaaggttg aggttcttct caacgataat
      721 tcgcagcacg ccaagtgcgg cacgacgcag cgcaaacggg tctttgtcgc ctttcggatg
      781 ctgaccgata ccgaagatac ccgccagagt atccatctta tcagcaatcg ccagcgcaca
      841 agctaccggg ttggacggca ggtcatcacc ggcaaagcgc ggctgatact gctcattcag
      901 cgcgacggct acgtcttccg cttcgccatc gtgacacgcg tagtgcatcc ccataacgcc
      961 ttgggtgtcg gtgaactcga agaccatgtt ggtcatcagg tcacacttag acagcaggcc
     1021 cgcacgggtt gcgtggttaa cgtcagcgcc aatctgttca gcaatccagc cagccagcgc
     1081 ctggatgcgg tcagttttgt cacgcagcgt acccagctgt tgctggaaca gcacggtttg
     1141 caggcgcggc aggttatctt ccagacgttt tttacggtcg gtattgaaga agaactcggc
     1201 atccgccaga cgtggacgaa cgactttctc gttaccagag ataatttgct gcggatcttt
     1261 cgattcgatg ttggcaacga agatgaagtt cggcagcagt ttgccatcgt tcgcatacac
     1321 cgggaagtat ttctggtcac ctttcatggt gtaaaccagg gcttcagccg gcaccgcgag
     1381 gaatttctct tcgaattttg cggtcagaac gaccggccac tccaccagcg aagccacttc
     1441 ttccagcagg ctttcgctta agtcagcgtt accgccaatc ttacgcgctg cttcttcggc
     1501 atcggcttta atcttcgctt tacgttcttc gtaatcggcg atgactttcc cgcgctcacg
     1561 cagaatttcc ggatactgat cggcgttgtc gatggtgaat tccggttcgc ccataaagcg
     1621 gtggccgcga atcacgcgat cggactgaat gcccagaata gttgccggaa tgactttgtc
     1681 gcccagcagc agggtcacgg tgtgcaccgg acgcacgaag tgcacgtcgc ttgcgcccca
     1741 acgcatcagt ttcgggatcg gcagtttcgc cagagaagtc gcaaccatat tcggcagcag
     1801 tgcttcggtg ctttcgccct tcacatgggc gcgatacagc agccattcgc ctttatcggt
     1861 agtcagacgc tcagcctggt caacggtaat accgcaacca cgcgcccaac cttctgccgc
     1921 tttgctcggt ttgccttcag cgtcgaacgc ctgggcaatc gccgggccgc gtttttcgat
     1981 ttcacgatcc ggttgcgctt ccgccaggtt agctactttc agcgccagac gacgcggagc
     2041 agcaaaccat tgaacggtgc cgtgtgcgag gccagcgtta tccagctcca cagtaaaatt
     2101 cgcagcaaag gactcaacca ggctgcgcag tgcttttggt ggcagctctt cagtgccgat
     2161 ttccaccaga aaagttttct cagacatagc cgcctcttac ttatctttgt tgcacatcgg
     2221 gaagccgagg gcttcacggg aagcgtagta tgcttctgcc actgctttgg tcagggtgcg
     2281 aatgcgcaga atgtagcgct gacgctcggt gacggagatg gctttacgcg catccagcag
     2341 gttgaagctg tgggcggctt tcagaatacg ctcgtaggct ggcagcggca gcggattttc
     2401 cagcgccagc agctgctgcg cttctttctc gtactgctca aagcaggtga acaggaagtc
     2461 cacatccgcg tattcgaagt tgtaagtgga ctgctccact tcgttctgat ggaacacgtc
     2521 gccgtaggtg gttttaccca gcgggccgtc gctccagacc aggtcgtaaa cgctgtctac
     2581 gccctgaatg tacatggcca gacgttccag accgtaggtg atctcgccgg taaccggttt
     2641 acactccaga ccaccaacct gctggaagta agtgaactgc gtcacttcca tgccgtttag
     2701 ccacacttcc cagcccagtc cccaggcacc cagagtcggg ttttcccagt tatcttccac
     2761 gaaacggatg tcgtgaatag tcgggtccat gcccagctct ttcagagaac cgaggtacag
     2821 ctcctgaata ttgtccggcg atggcttaat gaccacctgg aactgatagt agtgctgtaa
     2881 acggttgggg ttttcgccgt agcgaccatc ggtcgggcga cgagaaggct gaacataagc
     2941 agccgccatc ggttctggcc ccagcgcgcg cagacaggtc attgggtgag aggttcccgc
     3001 gccgacttcc atgtccaatg gttgaacaat ggtgcagccc tggcgagccc agtaatcctg
     3061 taaggtcagg atcaagccct ggaaggtcct ggtatcaaac ttttgcatat tatttcgtgc
     3121 tggatacgtg tggatttaaa ggaagggatc agtatacccg ctggatggaa gatatacagt
     3181 acgaaacggg aaaaagcagg gcttaacgca tggaaagatg caaaaaatgg ccatctgcgt
     3241 caaaagaaca gacaaactgc tctttacgga aggtataacc gcgcatttca tagcttccct
     3301 ggaactgttc gaaggcggtg acgtcgattt tttgctcgcc ggtgttataa cgttttgccg
     3361 cctgatcctt gcataacgct tccatattca acgaacgcag aggatcaacc tttacccgtt
     3421 gtgctttctg cacgggtgac ggtgtagaac aacccactag caccataagc gccattgccg
     3481 gaaagaaagc gttcatcatc attaaattac cgtctggttt gcaggtgagt cttattattt
     3541 atatcggtat agtggctgat atcttacgaa tttccttaag cctctataag ctgtagtgat
     3601 aaaactcaga ttattccagt gcgccaacca aatgatttat cggtcagact ttttttaacc
     3661 agatacagag aca
//
LOCUS       DAJVUJ010000252         3540 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.255, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000252 DAJVUJ010000000
VERSION     DAJVUJ010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3540
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.255"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            232..543
                     /locus_tag="OQQ48_004088"
     CDS             232..543
                     /locus_tag="OQQ48_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCS1954651.1"
                     /translation="MFNVITHPAALDELLELPDNLRGRMTRLIERLEQEGNKMKMPHS
                     RVIGGGLFELRVGDKNIARTLYAYATGNEIYLLHAFVKKTQKTPTKAIEIARMRLKEM
                     N"
     gene            543..830
                     /locus_tag="OQQ48_004089"
     CDS             543..830
                     /locus_tag="OQQ48_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCS1954652.1"
                     /translation="MKVKGVPFSQVKQKLLSTPEAVKGYEDADKELEMVEMLYEMREK
                     AGLTKSALAERMGLRPSAISRLESNPLGASMKTLTKYAHACGATIDIHAVY"
     gene            complement(1367..2554)
                     /locus_tag="OQQ48_004090"
     CDS             complement(1367..2554)
                     /locus_tag="OQQ48_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HCS1954653.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQHIVF
                     TLPCQYWSLVFHNRWLLAEMSRIAADVILEICHQADVEPRIFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTSGHTWKNLHFYARKVMSMWRYRITRLLSRKYPDLVMPDALAAEGSSKR
                     EWNRFLDTHYRRGWNVNVSRVMDNATHVAVYFGSYLKKPPVPVSRLEHYAGQDEIGLR
                     YNSHRTKREEYLLMSGDEFMERFSWHVADKGFRMVRYYGFLSPAKRRLLEEVVYIITE
                     TVRKTAMQITWRGMYQRLLKVDPLKCVLCGSQMRFTGLKRGYRLAEQVLMHEPLARMR
                     WCG"
     gene            complement(2554..2919)
                     /locus_tag="OQQ48_004091"
     CDS             complement(2554..2919)
                     /locus_tag="OQQ48_004091"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1954654.1"
                     /translation="MARSAKPRKRKPASQRSKLPRYVVKLHEDDFFDEEDAEVLRFDS
                     FDDAVECCADLNIPFFVDAGNKKLVFWFVRVDDEGYPEIARCTEREFATILSGISAGG
                     MYCPECGTVHWPDGVAPPF"
     gene            3004..3333
                     /locus_tag="OQQ48_004092"
                     /pseudo
     CDS             3004..3333
                     /locus_tag="OQQ48_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146522.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            3363..3446
                     /locus_tag="OQQ48_004093"
                     /pseudo
     CDS             3363..3446
                     /locus_tag="OQQ48_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
     gene            3446..>3540
                     /locus_tag="OQQ48_004094"
     CDS             3446..>3540
                     /locus_tag="OQQ48_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCS1954655.1"
                     /translation="MEQKWTSKALSDLARLYDFLVLASKPAAARMV"
BASE COUNT          926 a          919 c          835 g          860 t
ORIGIN      
        1 aaggtgaaat ctgtcaatca gtaccggctt acgccgggct tcggcggttt tactcctgtg
       61 ccatatgcaa caacggagtg ccgccttcca tgccgctaac gcggcatcaa aaaatccctc
      121 ttctccagtt gcccccctcc gaactcagag tgtattttca tttattgatg aaatttactg
      181 tttgcgcaat tttcctgttt tagtgtattt tgtcattctg gaggctataa tatgtttaat
      241 gtgattactc acccggccgc actggatgaa cttctagaat taccagacaa tctgcgtggt
      301 cgtatgacca gactgattga acggctggaa caggaaggta acaaaatgaa aatgcctcac
      361 agccgtgtaa ttggcggagg gctttttgaa ctgagagtcg gggataaaaa tatcgcaagg
      421 acactgtacg cttatgcaac gggtaacgaa atctatctgc tgcatgcatt tgtgaaaaaa
      481 acgcaaaaga caccaacgaa agctattgaa attgccagga tgcgtctgaa ggagatgaac
      541 tgatgaaagt aaaaggtgtt ccattcagcc aggtaaagca aaaactactc agtactccag
      601 aagctgtcaa agggtatgag gatgctgaca aagaactgga gatggtggaa atgttatacg
      661 aaatgagaga aaaagcggga ttaacaaagt cagcactggc cgaacgaatg gggttacgcc
      721 cgtcagccat cagtcgcctg gaaagtaacc ctttaggggc cagcatgaaa actttgacaa
      781 aatatgctca tgcctgtggc gcaaccatcg atattcacgc tgtctattga ggggtccgct
      841 tggaattcgg aaaataaacc acgttaagac tattttttag ataaatgact ttcaggaatc
      901 gccaagatgg cgggataaca gcggttttgg tgtcctaaat cgtacaattc tgtacatcca
      961 cgcaagggaa tgtacggaag taagccagac ggttatcgga cggtcagaac atttcgtaca
     1021 gcctttacgt tcagtgagtt agctggcctg gacgtaacca ctcgttagct caattctctt
     1081 agcgtgggaa aatttccgaa ttccaagcgg acccctttat ccttgccccg ctgaatgagg
     1141 aaacgctgtt ccgggggatt atgctgaacg tcttccgttc atcgatagga attaaaaccc
     1201 caaaaagatt aaaaaacacc acaaaacgga tgtttcttca acaccacttt tgctcatatg
     1261 aacggaaccg acgattaaac tggatggctc tgattgattc agggtatgaa tggcggtttt
     1321 ttgctccgtt tccctcaaaa tggacgctac ttaccctctg cggctctcag ccgcaccacc
     1381 gcatccgggc cagcggctca tgcatcagga cctgctctgc cagacggtag ccccgcttca
     1441 gccccgtaaa acgcatctga ctcccgcaca gcacgcactt cagcgggtca accttcagta
     1501 acctctgata catccctctc caggtgattt gcatcgccgt ttttctcact gtctccgtta
     1561 tgatgtacac cacttcttcc agtaaccgcc gtttcgccgg actcagaaaa ccgtagtacc
     1621 tcaccatacg gaacccctta tccgccacat gccaggagaa cctttccatg aactcatctc
     1681 cactcatcaa caggtattct tcccgttttg ttcggtgact gttgtaacgc agtccgattt
     1741 catcctgacc ggcataatgc tccagacgac tcaccggcac cggtggcttt ttcaggtaag
     1801 agccaaagta caccgccaca tgggtggcat tatccatcac ccgggatacg ttgacattcc
     1861 agccacggcg gtaatgcgtg tccaggaagc gattccattc ccgtttactg cttccttctg
     1921 ctgccagcgc atccggcatc accaggtcag ggtatttccg tgacagtaac cgtgttatcc
     1981 ggtagcgcca catgctcatc accttacggg cgtaaaaatg aagatttttc caggtgtggc
     2041 ccgacgtcac accaccggca gttgtcgata aatggatatg cggatgccac tgctggtcac
     2101 gcccccatgt gtggatcacc gtgaatatcc tcggctccac atctgcctga tggcagattt
     2161 ccagtatcac atccgctgca atgcggctca tctctgccag caaccaccgg ttgtggaaca
     2221 ccagggacca gtactggcag ggtaacgtga acacaatatg ctgccatggg cagtcgggga
     2281 ccaggctcag cagatactgt atccactgtg cgccagcctt caccccgcag tgcgggcagg
     2341 agcggctttt acaccggaag cagacctttt ttgtatggca acagtccggt gatgaacagc
     2401 accactgtgt ataccccatc agtgtggtcc cgcacgccat gattttggtc accgactcaa
     2461 tcaccaccgg acgtactgcc ccttccggct gcttctccag ccagttaagc cagcggtttc
     2521 cctgctgaaa gatatcggca aaacggggaa gcatcagaag ggcggggcga ctccgtccgg
     2581 ccagtgaacc gtgccacact ccgggcagta cataccgccg gcgctgatac cggaaagaat
     2641 ggtcgcaaat tcccgctccg tgcagcgggc aatttccgga tacccttcgt catcaacacg
     2701 tacaaaccag aagaccagtt ttttgtttcc ggcatccaca aagaacggaa tattcaggtc
     2761 tgcgcaacac tcaacggcat catcaaaact atcaaagcgc agaacttctg cgtcttcttc
     2821 gtcaaaaaaa tcatcttcgt gaagcttcac gacatagcgg ggaagtttgc ttctttgaga
     2881 ggcgggttta cgtttacggg gtttagctga acgggccata taaccacctg aaagacaatg
     2941 acattgcctg tttttataac ggtaattgca gaccatgaca agccgcagcc gtcaggctgc
     3001 ctactcgagc ttcagctcaa taaaggcgaa tatcgacata aattaccccg atggatcttt
     3061 tttgcaaatc agggtgaatt cacgaccggt gattacgaag aaatcatgaa taaggccagc
     3121 gccctgagtc tggtatcgaa tgcaatcctg tactggaata catcaagaat aagcaatatt
     3181 gttgaagggt tacgtgaaaa tggtgaggtt attgatgatg acgtattagc ccatatatca
     3241 ttactgccct ataaacacgt attacctaat ggtacttatt ttgttgatgg agaaccttac
     3301 ggtactgagc ctaccgaaag agcaaaaccg taagtttctg ttcaaatgct cccgctggtc
     3361 catccggaca gattatcgac catcaggctg tacaggcctg ggcggacagc ctcagtactg
     3421 acaatccgtt accggtgcca cgctgatgga acagaagtgg accagtaagg cgctttctga
     3481 tttggcgcgg ttatatgatt ttctggtgct ggccagtaaa cctgctgccg ccagaatggt
//
LOCUS       DAJVUJ010000253         3488 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.256, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000253 DAJVUJ010000000
VERSION     DAJVUJ010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3488
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.256"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            1..1947
                     /locus_tag="OQQ48_004095"
                     /pseudo
     CDS             1..1947
                     /locus_tag="OQQ48_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
     gene            complement(1990..2436)
                     /gene="ivy"
                     /locus_tag="OQQ48_004096"
     CDS             complement(1990..2436)
                     /gene="ivy"
                     /locus_tag="OQQ48_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HCS1954656.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            2617..3387
                     /gene="yafV"
                     /locus_tag="OQQ48_004097"
     CDS             2617..3387
                     /gene="yafV"
                     /locus_tag="OQQ48_004097"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HCS1954657.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAI
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMMALREYREKFPAWQDADEFRLR"
BASE COUNT          778 a          891 c         1001 g          818 t
ORIGIN      
        1 atgattatca aaaaagagta cggctgactg gagttctcag cttatgccca gtctcgcgtg
       61 ctgcaaaaac tctccggcgt gagcgggatc ctggcgatta ccgtcggcgt gccgaactca
      121 ttaggcccgg gcgagctgct gcaacattac ggcactgacg agcagaaaga tcactatctg
      181 ccgcgtctgg cgcgtggtca ggagatccct tgctttgcac tgacaagccc ggaagcgggt
      241 tccgatgcgg gcgcgattcc ggacaccggg attgtctgca tgggcgaatg gcagggccag
      301 caggtgctgg ggatgcgtct gacttggaac aaacgctata ttacgctggc accgattgcg
      361 actgtgcttg ggctggcgtt taaactctcc gacccggaaa agctgctggg tggcgcagaa
      421 gatttaggta ttacctgtgc gctgatcccg accaccacgc cgggcgtgga aattggtcgt
      481 cgccacttcc cgctgaacgt accgttccag aacggaccga cgcgcggtaa agatgtcttc
      541 gtaccgatcg attacatcat cggtgggccg aaaatggccg ggcaaggctg gcggatgctg
      601 gtggagtgcc tctcggtagg ccgcggcatc accctgcctt ccaactcaac cggcggcgtg
      661 aaatcggtag cgctggcaac gggcgcgtat gctcacattc gccgtcagtt caaaatctct
      721 atcggaaaga tggaagggat tgaagagccg ctggcgcgta ttgccggtaa tgcctacgtg
      781 atggatgctg cggcatcgct gattacctac ggcattatgc tcggcgaaaa acctgccgtg
      841 ctgttggcta tcgttaagta tcactgtacc caccgcgggc agcagtcgat tattgatgcg
      901 atggatatca ctggtggcaa aggcattatg ctcgggcaaa gcaacttcct ggcgcgtgct
      961 taccagggcg caccgattgc catcaccgtt gaaggggcta acattctgac ccgcagcatg
     1021 atgatcttcg gacaaggagc gattcgttgc catccgtacg tgctggaaga gatggaagcg
     1081 gcgaagaaca atgacgtcaa cgcgttcgat aaactgttgt tcaaacatat cggtcacgtc
     1141 ggtagcaaca aagttcgcag cttctggctg ggcctgacgc gcggtttaac cagcagcacg
     1201 ccaaccggcg atgccactaa acgctactat cagcacctga accgcctgag cgccaacctc
     1261 gccctgcttt ctgatgtctc gatggcagtg ctgggcggca gcctgaaacg tcgcgagcgc
     1321 atctcggccc gtctggggga tattttaagc cagctctacc tcgcctctgc cgtgctgaag
     1381 cgttatgacg acgaaggccg taatgaagcc gacctgccgc tggtgcactg gggcgtacaa
     1441 gatgcgctgt atcaggctga acaggcgatg gatgatttac tgcaaaactt cccgaaccgc
     1501 gtggttgccg ggctgctgaa tgtggtgatc ttcccgaccg gacgtcatta tctggcacct
     1561 tctgacaagc tggatcataa agtggcgaag attttacaag tgccgaacgc cacccgttcc
     1621 cgcattggtc gcggtcagta cctgacgccg agcgagcata atccggttgg cttgctggaa
     1681 gaggcgctgg tggatgtgat tgccgccgac ccaattcatc agcggatctg taaagagctg
     1741 ggtaaaaacc tgccgtttac ccgtctggat gaactggcgc acaacgcgct ggcgaagggg
     1801 ctgattgata aagatgaagc cgctattctg gtgaaagctg aagaaagccg tctgcgcagt
     1861 attaacgttg atgactttga tccggaagag ctggcgacga agccggtaaa gttgccggag
     1921 aaagtgcgga aagttgaagc cgcgtaattg aataacggag ccgaaaggct ccgtttcttt
     1981 atccgctaat tatttaaaat taaagccatc cggatggttt tccaggctgc cggtcaacgc
     2041 cgcgaacagc accgttttac catcaatcga aagcgcatcg ttcacattca gccaggtgag
     2101 tttctcttgc gacgttttct catcaatagt cgagaacagc cccgtcatct gattagattt
     2161 ctcggaccac atcacagcga tacgttgcga gccacagtca tgcggtttgc acgcgctcat
     2221 cacctgatac gtctcatctc ccaacgttac ggtttgtgcg ggagtataag taccgccttt
     2281 catcacccag gcaggcagct tatgcccttg taccatctga ttaaatgcag ctttggtggt
     2341 ttcgcccttt gcaaggctgc taatggttaa atcatcctgc gccattgcac tggtggcgat
     2401 gaccagagcg gcgactgtcg ttattgcctt aaacatcatt cctcccgagc ttatcctgcc
     2461 catgttatta accttctaaa gcctggcgat aacacgataa tttgcaaatc agatatttcc
     2521 aatatcagca tttgttatca atttattctg cgctaaggca actgcctgat aaggtgttaa
     2581 agttataaaa cccttcgcat aaaggagttg tacaccgtgc ctggtttgaa gattacgctt
     2641 ttgcagcaac cactggtgtg gatggatggc cctgccaacc tgcgtcattt tgatcgtcaa
     2701 ctggaaggta ttaccgggcg cgatgtgatc gttctaccgg agatgtttac cagcggcttt
     2761 gccatggaag cggcagcttc gtcgctagca caagatgacg tagtgaactg gatgacagct
     2821 aaggcgcagc agtgcaatgc gctgatagca ggcagtgttg cattacaaac ggagtctggt
     2881 tcggttaacc gctttttgct ggttgagccg ggcggcacgg tacattttta tgataagcgt
     2941 catctgttcc gcatggcaga tgagcatcta cattataaag cgggcaatgc gcgagtgatt
     3001 gtggaatggc gcggctggcg tattttgccg ttggtgtgct acgacttacg ttttccggtg
     3061 tggtcgcgca atctcaacga ttatgacctc gccatctacg ttgccaactg gcctgctccg
     3121 cgctctctgc actggcaggc attgctgacg gcccgcgcga ttgagaatca ggcgtatgtg
     3181 gcgggatgca atcgcgtcgg cagcgatggc aacggctgcc attatcgcgg tgacagccgg
     3241 gtgattaatc cacaaggcga gattatcgct actgccgacg cgcatcaggc aacgcgcatt
     3301 gatgcggagc tgtcgatgat ggcattgcgg gaatatagag aaaagtttcc ggcatggcag
     3361 gatgcggatg agtttaggtt gcggtgagtg ggggttgtgt ttaaggctgg gggagagtcg
     3421 gggtaagact acgaaagccc gctctccgca aggactgacg ccagatagtt tctgtccata
     3481 gctgcttt
//
LOCUS       DAJVUJ010000254         3474 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.257, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000254 DAJVUJ010000000
VERSION     DAJVUJ010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3474
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.257"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            577..1293
                     /locus_tag="OQQ48_004098"
     CDS             577..1293
                     /locus_tag="OQQ48_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1954658.1"
                     /translation="MNDNSLLRNSSLFIAYMGCVGWVSAYSYGWGTSFYYGFPWWVVG
                     AGLDDVARSLLYAIIVMGILFTGWGIGILFFLLIKKRSKIQDLSFFRLFFAITLLFFP
                     VIFELLILKQYFILPLSLSCIISSLVISIIIRIYGRIFSVSCFSDIPFVREHRIKLIM
                     AGFLVYFWLFSFLVGWYKPQLKKEYQMLCYNNSWYYVLARYDSRLVLSSSFKDDSNRF
                     LIFNTEQSGFYEINDVYVRK"
     gene            1845..2249
                     /locus_tag="OQQ48_004099"
     CDS             1845..2249
                     /locus_tag="OQQ48_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1954659.1"
                     /translation="MNTALIVALMCMWYAVPAAAKETLLAMPRNSTEHCYAEINVHGP
                     YGVYFRVVPHPPGGKSWVECNSDYYYSDKPPGVQILGTRAGCRVYGICGTTSTLHVAG
                     RGVVCIKNICSPRGMIIHRIRKRPVVAVSDEM"
     gene            2312..2946
                     /locus_tag="OQQ48_004100"
                     /pseudo
     CDS             2312..2946
                     /locus_tag="OQQ48_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(2988..3197)
                     /locus_tag="OQQ48_004101"
                     /pseudo
     CDS             complement(2988..3197)
                     /locus_tag="OQQ48_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            3262..>3474
                     /locus_tag="OQQ48_004102"
     CDS             3262..>3474
                     /locus_tag="OQQ48_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1954660.1"
                     /translation="MSRKTQRYSTEFKAEAVKTVPENQLSISEGASRLSVPEGTLGQW
                     VTAARKGLNTPGSRTVAELESEVMQLR"
BASE COUNT          968 a          731 c          793 g          982 t
ORIGIN      
        1 ttgtgggcca taaatattcc aaaaaaatag cccgctccgt ttattaccgg aacgggcgag
       61 agcatgacat tgtctgtaat tataatgaga ctgctgattc tacttatttt taatttcaag
      121 aaaagcgttt tctacaaaga attatgagat ttaatgtttt gtttagacaa tattgtcatt
      181 ctgttcaata atgaaagtct tcatcaacaa caagtaaaaa caacgtccct ctcatcgaac
      241 cggacttgca gatttcccgc atccggctct cttgcaacac atcagacctt cacgttcggg
      301 cggttatggg gaaggtccag caagctgtat gagccctaac tgtttgaata ccagtagatt
      361 accagaaact tctatcgatt gaagacatta ccaactagcg ttactaaatg ttgaccaagt
      421 tttgttcact tgtctggttt tgatagtagc attctgcaga gtattttgat atgataaata
      481 tgcattaaca atgatattat tgttaatttc ctagcatgaa aattaaaaga tcaacttttt
      541 atctcgaata ttttttacat tatcagttgg tttcttatga atgacaattc attgcttaga
      601 aattcttcat tattcatcgc ctatatggga tgtgtagggt gggtcagtgc ttattcttat
      661 ggctggggaa cctcttttta ctatggattt ccatggtggg ttgttggtgc aggtttggat
      721 gatgttgcca gaagtttact ctatgctatc atagtaatgg gtatactttt taccggttgg
      781 gggattggta ttttattttt tcttcttata aagaaaagaa gtaaaataca agacttgagt
      841 ttttttagac tattttttgc tattacccta ctgtttttcc ctgtaatatt cgaactttta
      901 attctcaaac aatatttcat acttccatta tcactttcat gcattattag ttcattggtt
      961 atttcaataa taattcgcat atatggacgg atcttttctg tatcatgttt ttcagatatc
     1021 ccttttgtta gagaacaccg cattaagtta attatggctg gttttttggt ttatttttgg
     1081 cttttttcct ttcttgttgg ctggtataaa cctcaattaa aaaaagaata tcagatgctt
     1141 tgttataata atagttggta ttatgttctt gctcgttatg attcacggct cgttttgtct
     1201 agttcattta aagatgacag taacagattc ttaatattta atacagaaca gtcgggattt
     1261 tatgaaatca atgatgttta tgtcagaaag taataatcct ctcccacaaa gtgaattaaa
     1321 cgaatatata atgcgttcga agtatatgta ttgagctgtc atactgagaa acaaacctta
     1381 gggcagcaca attaaagcca gaaacaacta tgcaagaaag ttactaaata agtgctcgga
     1441 acagtaaaac actcaggggg gactcagtcc agattatgta atcagataaa ttgccaaaca
     1501 tcacaaatca ccagcaggac tgagtgatgc cgagcattgc accaataccc cgtgatgaac
     1561 gatgcctgat gcagaaagct ccctataaaa caaagaacaa aaactacgac cggcgattca
     1621 ccgtcatact gatgctgcat cgtggtgaca ctgtctgtca ctttcatgtt gactgaacaa
     1681 ctctgctgcg aagaaggtgt accgccggcg ggatttgatt gtccggcgtc tagcttccac
     1741 ttccctgatg gaactgaaag ttgttatggc cggtcagctt gccctgtccg caggacatga
     1801 ggtgctgtga cggacagcac cagtaacgga aagtgacaat aaaaatgaat actgcgctga
     1861 tagttgcact aatgtgtatg tggtatgcgg tacccgctgc tgcaaaagag actttactgg
     1921 ctatgccacg taattcaaca gaacattgct atgcggagat taatgtgcac ggaccatatg
     1981 gggtgtattt ccgagtggtc ccgcatcccc ccgggggaaa gagctgggtc gaatgtaaca
     2041 gtgactacta ttactctgat aaaccaccgg gagtacagat actcggaacc cgtgcagggt
     2101 gtcgggtata cggaatctgc gggaccacct cgacactgca tgtggctgga agaggggttg
     2161 tgtgtattaa aaatatatgc agtccacgtg ggatgataat tcacaggata cggaaacgtc
     2221 ctgtagtggc tgtaagcgat gagatgtgat cttatccagc aatagtggac acgcggctaa
     2281 gtgagtaaac tctcagtcag aggtgactca catgacaaaa acagtatcaa ccagcaaaaa
     2341 aacacgtaaa cagcattcgc ctgaattttg cagtgaagcc ctgaagcttg ctgaacgcat
     2401 cggtgttgct gccgcagccc gtgaactcag cctgtatgaa tctcagctct atgcgtggcg
     2461 cagcaaactg cagcaacaga tgacctcgtc tgagcgtgaa agtgaactgg ccgctgaaaa
     2521 tgcccgcctg aagcggcaac tggcggtgca ggcagaggaa ctggctatcc tccaaaaggc
     2581 cgcgacatac tttgcgaagc gcctgaaatg aagtatgtct ttattgaaaa acatcaggct
     2641 gagttcagca tcaaggcaat gtgccgcgcg ctccgggtgg cccgcagcgg ctggtatacg
     2701 tggtgtcagc ggcggacagg gataagccct cgtcagcagt tccgtcagca ctgcgacagc
     2761 gttgtcctcg cggcggcctt tacccggtca aaacagcgtt acggtgcacc acgcctgacg
     2821 gatgaactgc gtgctcaggg ttaccacttt aacgtaaaaa ccgtggcggc aagcctgcgc
     2881 cgtcaggggc tgagggcaaa ggcctcccgg aagttcagcc cggtcagtta ccgcgcacat
     2941 ggcctgaggt gtactggcaa tagcggacac caccatttgt tctttttcta agcagtcatc
     3001 tgataatatt ttatcctgaa ggctgccggg gagatattcc ccagacgaga gtgacgacgc
     3061 tgacgattgt agaaaatctc aatgtattcc cgtattactg agatggcctc atcccggctc
     3121 ttaaaacgat agtggctcag gctttcattt ttcagcgttc cccagaagct ttccatcgga
     3181 gcgttgtcgt aacagtttga ggtagcctga gtttaacgga cactccttcc tgaaatagaa
     3241 tggcatcaga aggagccaat aatgagcaga aaaacccaac gttactctac ggagtttaaa
     3301 gccgaagccg tcaaaacggt tcctgaaaat cagctttcga tcagtgaagg cgcttcccga
     3361 ttatctgttc ctgaaggcac tttaggacaa tgggttaccg ctgcaagaaa agggctcaat
     3421 acaccaggct cccgcacagt ggctgagctg gaatctgaag ttatgcaact gcgt
//
LOCUS       DAJVUJ010000255         3459 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.258, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000255 DAJVUJ010000000
VERSION     DAJVUJ010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3459
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.258"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..322
                     /locus_tag="OQQ48_004103"
     CDS             <1..322
                     /locus_tag="OQQ48_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1954661.1"
                     /translation="HHGADSFYPLGSMPRRFSSVRPNSLSQLARLMPSLCASLSNCSF
                     SSGEIRILNCGDCPSPLGLLSRLIIDKWSPIELAFPLLGGHLNTRALKKAKPRKCHYH
                     SQGF"
     gene            348..548
                     /locus_tag="OQQ48_004104"
     CDS             348..548
                     /locus_tag="OQQ48_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HCS1954662.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGETYAQ"
     gene            538..759
                     /locus_tag="OQQ48_004105"
     CDS             538..759
                     /locus_tag="OQQ48_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1954663.1"
                     /translation="MHNKTTPNAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
     gene            752..985
                     /locus_tag="OQQ48_004106"
     CDS             752..985
                     /locus_tag="OQQ48_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1954664.1"
                     /translation="MRDDRFNSLKQEFPGVPDDAADALSSMPELIRAAFFLLSTREYK
                     STGLDVLNIAADYAEYVAEARYRRKFPEDVSHA"
     gene            978..1211
                     /locus_tag="OQQ48_004107"
     CDS             978..1211
                     /locus_tag="OQQ48_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1954665.1"
                     /translation="MRDIYHETIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            1217..1516
                     /locus_tag="OQQ48_004108"
     CDS             1217..1516
                     /locus_tag="OQQ48_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCS1954666.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGTVMIKGVEERRVTY
                     RREGYEYDCVMPVYQFRRDFSLVQTAPHNVPTSNARARANIQKLKTMINGFRGKK"
     gene            1513..2913
                     /locus_tag="OQQ48_004109"
     CDS             1513..2913
                     /locus_tag="OQQ48_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCS1954667.1"
                     /translation="MKSAPNLKKQPYDKMTEVIIFAGSDAWAHAKQWQEQDGRLAGDN
                     VPPVVLADDQLDELADLRIIDEGRYCVRLYKAGHIRPSNINAIAHKLAAAGVTDANYY
                     PEGMHSHMRENWREYLERVRGKEPVEEKNHQRKTTLPMSVGSTGYDTQLDYVVKGIIP
                     AVSLCSIYGASGSYKSFLAGSWACHVATGRQWGGRRVAHGAVLYVVGEGGIGVPRRVK
                     AWEVVHDEQVKNLYLVNRPIFPAAPLDVDEMVIAARQVERETGKPVRMIILDTLARCF
                     GGNDENDSRDMGAFIRGCDELKRRTGATVLVVHHSGKDETKGARGSSAFRASLDAEYR
                     IRREDAGSKALVISCTKMKDAEELKEAAYDLRVVELFTDADGELITSLVVVDDPRPPV
                     ELERIEEAGNKTENHTALWGCIRSRTQNGDKCTIPLLRDDMKKLGYEMKNFRRWLYKL
                     EKDGVIRIDGDDVAPL"
     gene            3114..3365
                     /locus_tag="OQQ48_004110"
     CDS             3114..3365
                     /locus_tag="OQQ48_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1954668.1"
                     /translation="MTQKRRDRTEPKYKALDMTEHTLKVAIRTIDRHTREGYAKEHPD
                     LISAFMTTAAANFATLTEREIAEAEQVTTINVKTGEVEL"
     gene            3362..>3459
                     /locus_tag="OQQ48_004111"
     CDS             3362..>3459
                     /locus_tag="OQQ48_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCS1954669.1"
                     /translation="MTAQIAAYGRLVDDPQVKQTSKGTPMTLARMAV"
BASE COUNT          952 a          804 c          956 g          747 t
ORIGIN      
        1 acaccacggc gctgatagtt tttatccttt gggttctatg ccgcgccgct ttagttcagt
       61 gcgccccaac tctttaagcc agttggctag gcttatgccg tcgctctgtg cttctttgtc
      121 gaattgctct tttagctctg gagaaattcg cattctgaat tgtggggatt gcccgtcccc
      181 tttagggctt ttatcgcgtt tgattattga caagtggtca cctattgaat tagcctttcc
      241 attgttaggt ggccacctta acacaagagc acttaaaaaa gcaaagcccc gcaagtgtca
      301 ttaccactcg cagggcttct aaccaacaac gtaaactagg agccgttatg gttgccgtaa
      361 atcatatacc acaccttgta cacacacaaa cggcctttgt gtggcgtttt ctggccctga
      421 gtgccggaga atctcaaatc atccacgtaa ccgcctggac ggaacgcgaa gcgcgtagcc
      481 gttgcccgtc cggttgtgtt gctgtattcg ccgcccgtat tcgtcagggg gaaacctatg
      541 cacaataaaa ccacaccgaa cgcagcagcc gccgcactca ctacgctgat gcacgcgctt
      601 attgatattg aatgcactgc agagcttgcg cagggagaag aacagaaaga ccggacacag
      661 ttcgccctgg aatgtatccg atacatcgca acgcggtcgc tgaatgacgc taaaaatatt
      721 cttgttgctg attgtgaaaa tggggggggt tatgcgtgat gatcgtttta attccctgaa
      781 acaggaattt cccggcgttc ctgatgatgc ggctgatgcg ctttcgtcaa tgccagaact
      841 tattagagcg gcttttttct tactttccac gagagaatat aaatcaacgg ggcttgatgt
      901 actgaatatc gccgccgatt atgcggaata tgtggcagag gcgcgttaca gaagaaaatt
      961 tcctgaggat gtaagccatg cgtgatattt accacgaaac aatagaccgc gcatttcttg
     1021 cactttctca cagtgaaaac atgctggaaa tattgcgcat atggcttgaa acacttggcg
     1081 acaatgaacg cgacaaacaa aaatcaagaa ttgccacggc attaataacg cttcttgagc
     1141 ctgtaataat ggaactgcaa gaaatagatc tattgcacga cagatataaa gaacagcaca
     1201 ccggagaata aaaataatga aacttaaata ttctggctta actgccagtg gcaacactca
     1261 ccctaaattt acgcgcggtg atatttaccg cgaccagtac ggcggcacgg taatgattaa
     1321 gggcgtggaa gaacggcgtg taacctaccg ccgtgaaggt tacgaatatg attgcgtgat
     1381 gcctgtttat cagttccggc gtgatttttc tctggtacag accgcgccgc ataacgtgcc
     1441 caccagcaac gccagggcac gggcaaacat ccagaagctg aaaaccatga ttaacggatt
     1501 caggggcaag aaatgaaaag cgcaccgaac ttaaaaaaac agccttacga caagatgacc
     1561 gaagtcatta tttttgcggg tagtgatgcc tgggcacatg caaaacagtg gcaggaacag
     1621 gacgggcgac tggctggcga taatgtgcct cccgttgtgc tggctgatga tcaactggat
     1681 gaactggcag acctgagaat catcgacgag gggcgctatt gtgtccggct gtacaaggca
     1741 ggccacatca ggccatcaaa tattaatgcc attgcgcaca agctggcggc ggcgggtgta
     1801 actgatgcga attattaccc cgaagggatg cacagccata tgcgggagaa ctggcgcgaa
     1861 tacctggaac gggtgcgcgg gaaagagccg gtggaagaaa aaaaccacca gcgaaaaacc
     1921 acgctaccga tgagcgttgg atctaccgga tacgacacgc aactggatta cgtggttaag
     1981 gggattattc cggcggtatc gctatgcagc atatacgggg ctagcgggtc ctataaatca
     2041 ttccttgccg gatcgtgggc gtgccatgtt gccactggtc gccagtgggg aggccgcagg
     2101 gttgcacatg gtgcggttct ctatgtggtt ggtgaaggcg gtatcggcgt tccgcgtcgt
     2161 gtaaaagcct gggaggttgt gcacgatgag caggtgaaaa atctgtatct ggtaaaccgc
     2221 cccatctttc cggctgcccc gcttgatgtt gatgaaatgg ttatcgctgc ccgtcaggtg
     2281 gagcgggaaa cgggtaaacc tgtacgcatg attattctgg atacgctggc gcgttgcttt
     2341 ggtgggaatg atgaaaatga ttcccgtgat atgggggcgt ttatccgtgg ttgtgacgaa
     2401 ctgaaacgac gcacaggggc cacggtgctg gtggttcacc attccggcaa ggatgagacg
     2461 aaaggcgcgc gcggttccag tgcatttcgt gcttcgctgg atgctgaata ccggatacgc
     2521 agggaggacg caggaagcaa agcgctggtt atctcatgca ccaaaatgaa ggacgcggag
     2581 gaactcaaag aagccgcata tgacttacgc gtggtggagc tttttaccga cgctgacggt
     2641 gaattaatca cgtcgctggt ggtggtggat gatccgcgcc ctcctgttga actggagcgc
     2701 atcgaggagg cagggaacaa gacggaaaac cataccgcgc tatgggggtg catccgttca
     2761 cgcacacaga acggcgacaa gtgcacgatc ccgctgttac gtgatgacat gaaaaagctg
     2821 gggtatgaaa tgaaaaactt ccggcgctgg ctgtacaagc tggaaaaaga tggggttatt
     2881 cgtatcgatg gggatgatgt agcgccgcta taaaagtgag gagcaaaagc gagggggata
     2941 gaaagagggc caaaattagc ccgctctccc tcacttttcg acctgtatac atcctcaaaa
     3001 gtgaggggta aaaaaatact tataaaacac acacatagaa aaaccgaaaa tcccaactgc
     3061 gacgaagtga gacgcttgaa aaagtgaggc gaaaaagtga gaggttgcga gaaatgaccc
     3121 aaaaacgcag agacagaaca gagccaaaat ataaagcgtt agacatgact gagcacacct
     3181 taaaggtggc aatcagaacg atagaccgcc acacgcggga aggatacgcg aaggaacatc
     3241 ccgacctgat aagcgcattc atgaccacgg cggcggcaaa ctttgccacg ctgacagaac
     3301 gggagattgc cgaagcggaa caggtaacaa ccatcaacgt taaaaccgga gaggtggaat
     3361 tatgacggca cagatagccg cttacgggcg gctggtggac gacccgcagg taaaacagac
     3421 cagcaagggc acaccgatga cgctggcgcg tatggcggt
//
LOCUS       DAJVUJ010000256         3411 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.259, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000256 DAJVUJ010000000
VERSION     DAJVUJ010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3411
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.259"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(21..323)
                     /locus_tag="OQQ48_004112"
     CDS             complement(21..323)
                     /locus_tag="OQQ48_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1954670.1"
                     /translation="MLEIVLSPVKAQQFTVTLGAQVCTIRLNQRTTGMYIDIIVNGEP
                     CLYGVLCLNNNRIVRYGYLPFQGDLFFTDTEGNSDPDWRGLGSRYRLYWLLPEELI"
     gene            complement(323..1033)
                     /locus_tag="OQQ48_004113"
     CDS             complement(323..1033)
                     /locus_tag="OQQ48_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000010340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1954671.1"
                     /translation="MSFSFDNISLNNFSLSESNVLSAVRGGGVLGLINSVLAPSFGIY
                     YARNDPAGAHQKGGKPFSPDSFVVVEVGAEASVSTVPVEQGAYTTFNKIQRSPELHVT
                     FIVEGWTAFSGSVPNLTNFSTTSRTNVLETLERMRTTAGLYDIETPDKTWTSCDLVKY
                     DYRMRSNNGPTLLTVTAVFQTVMNTGEVSVGSTDKSSPTDNDKAKGAASVKTQPVTAS
                     VTQPSDADRRSATNRGIT"
     gene            complement(1030..3051)
                     /locus_tag="OQQ48_004114"
     CDS             complement(1030..3051)
                     /locus_tag="OQQ48_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HCS1954672.1"
                     /translation="MAQVLDELVLSLGIDDRDFTAGEQAVHAALNRLTTVMEGVADTF
                     SQGQKKTSESLEKTGKDADKTARRMEDAGKRASRFFSGIRSEILALAGVSLTLGGLKN
                     LVTGFARDLNRLSVESDAFGMKARNLDGWLRAARANNVDEGEMSGAFSRLANAKAAFR
                     AGRSFDPVLQDLFQVAARAGISVDLNTDSTEAIMRKLAVIFPRLTKSEQTAYGNALGF
                     SYAGQQFLGSGHALKDVDEFTSRSQVTPGRTALARKLRKELVELDQTWSGIGMTISTA
                     LMPYALKLSHWLETLGDWMQQHPEEVNRFITTFLNDVESVASLANKAAGELGGWQNVI
                     ITLIGLKVASWVRGLTKALSGPGGLLFAITALYPVVDGLLTSIVGRENKDWLDSHGFF
                     WASDGTFFFNKKEMETYQTKLDVGEKPGNITRAQSPTVWQQGMLDTQASQATGREAAF
                     GESWLQGMRATQEKLGNAMQNRQRPTKTGEALLGWLQPKLSQLEEKYNLPPGLLRSVA
                     ITESRGNQFAVSPAGAMGLFQFMPKTASEFGLRGNDAFDPEKAADAAARKLGGLIRFF
                     HGDLAKALAAYNWGEGNVQRKGLAAAPEETRNYIPRVLANLPYPGAAMAIRSLHPAPV
                     SQSTVTETTHIGTLNVTTTSDNVKGITDDARRRITNSALVSVYSSGVTG"
     gene            complement(3035..3217)
                     /locus_tag="OQQ48_004115"
     CDS             complement(3035..3217)
                     /locus_tag="OQQ48_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCS1954673.1"
                     /translation="MVDYVNVPRTIATVISSGKASKAELDSVLGVQDLWDLLEIIHVD
                     AHNEQVIQENRNGAGT"
BASE COUNT          798 a          993 c          814 g          806 t
ORIGIN      
        1 tattttacgt tgtctgtagc tcatatcagt tcctcaggta acagccagta gagccggtac
       61 cgggatccga gcccccgcca gtcagggtca ctattcccct ccgtgtcggt aaaaaacagg
      121 tccccctgaa acggcaggta tccgtaccgg acaatccggt tattattcag acacagcacg
      181 ccatacaggc acggttcacc attaacaatg atatcgatat acatccccgt agtacgctga
      241 ttcaggcgaa tggtgcagac ctgagcgccc agtgtcaccg taaactgctg ggctttaacg
      301 ggagataaaa caatttccag catcaggtga tccccctgtt cgtggcactt cgtctgtcag
      361 cgtctgacgg ttgtgtcacc gacgccgtaa ctggctgcgt tttaaccgat gcggcccctt
      421 ttgctttatc gttgtccgtg ggagacgact tatccgtact tcccactgac acctctcctg
      481 tattcattac ggtctgaaat accgcagtaa ccgtcagtaa ggtcggacca ttattacttc
      541 gcattcggta gtcatatttc acaaggtcgc aggatgtcca tgtcttgtcc ggcgtctcaa
      601 tatcgtagag tcctgccgtg gtacgcatcc tttcaagcgt ttccagcaca ttcgtccgcg
      661 aggtggtgga aaaatttgtc aggttcggga cggatccgga aaaggccgtc cacccctcta
      721 caatgaaagt cacatgcagt tccggcgatc tctggatttt attaaaggtg gtataagccc
      781 cctgctccac ggggacggtg gaaacagaag cctccgcgcc cacctcaacg acaacaaaag
      841 aatccgggga gaaaggtttg ccacccttct gatgggcacc tgccggatca ttccgtgcgt
      901 aataaatacc gaatgacggt gccagtacac tattaatgag tcccaggaca ccgccgccac
      961 gaactgcact cagtacgtta ctttcactga gcgaaaagtt attcagggaa atattatcga
     1021 aagaaaaact catcctgtta ccccgctgga ataaaccgac acaagcgccg aattcgtgat
     1081 acgccgacgt gcgtcatcgg taataccctt cacattgtcc gacgttgtgg tgacattcag
     1141 tgtcccgata tgcgtggttt ccgttacggt ggactgcgat acaggcgcag gatggagcga
     1201 ccgtatggcc attgccgcac ccggataagg caggttcgcc agcacccgcg gaatatagtt
     1261 gcgggtctcc tccggagcag cagccagtcc cttacgctga acatttccct ctccccagtt
     1321 atatgccgcc agagccttag ccagatcgcc atgaaaaaaa cgtatcaggc caccaagttt
     1381 tctcgcggcg gcatcagcgg ctttttcggg atcaaaggca tcgttccccc tcagaccaaa
     1441 ttcactggcc gtcttcggca tgaactgaaa cagtcccatc gcaccagcag gtgagacggc
     1501 aaactgatta ccacgggatt cggtgatcgc aacgctgcgc agcagtccgg gcggcaggtt
     1561 atatttttcc tccagttgag acagtttcgg ttgcagccag cctaacaggg cttccccggt
     1621 cttcgtcggt cgctggcggt tttgcatggc attaccgagt ttttcctgcg tcgcacgcat
     1681 accctgtagc caggattcac cgaaggctgc ttccctcccg gttgcctgag aagcctgagt
     1741 atccagcatg ccctgctgcc atactgtggg tgattgtgcc cgggtgatgt tgccaggctt
     1801 ttctccgaca tccagttttg tctgatacgt ctccatctct ttcttattga aaaagaaagt
     1861 cccgtctgaa gcccagaaaa aaccatgtga atccagccag tccttatttt ccctgccaac
     1921 aatggatgtc agtaatccgt caacaaccgg gtaaagcgcc gttatcgcaa aaagaaggcc
     1981 tccggggcca ctgagggctt ttgtcagccc ccgcacccat gacgccactt tcagcccgat
     2041 cagcgtaata atgacattct gccagccccc cagttctccg gcagccttat tcgccaggga
     2101 ggccactgac tcaacgtcat tcagaaatgt ggtgataaac ctgttcactt cctccggatg
     2161 ttgctgcatc cagtcaccga gtgtctccag ccagtggctg agcttcagcg cgtacggcat
     2221 caacgcggtg ctaatcgtca tcccgatgcc ggaccaggtc tgatcgagct caacaagctc
     2281 ttttcgtagc ttacgggcca gcgctgtacg ccccggcgta acctgcgaac gggaagtaaa
     2341 ttcgtccaca tctttcagcg catgaccgga gccaagaaac tgctgtccgg cataactgaa
     2401 gcccagcgcg ttgccgtagg cagtttgttc ggactttgtc agtcgcggaa aaataaccgc
     2461 cagcttgcgc atgatggctt cagtgctgtc agtatttaaa tcaacactaa tgcccgccct
     2521 ggctgcaacc tgaaacaaat cctgtaacac aggatcaaag gaccttccgg ctctgaaggc
     2581 cgcttttgca tttgcaagcc gggaaaatgc cccagacatt tccccttcat caacattatt
     2641 tgcccgcgca gcccgtaacc atccgtccag atttctggcc ttcatgccaa aagcatcgga
     2701 ttccacggac agacggttca gatcacgggc aaaacctgtt accaggttct tcagcccccc
     2761 cagcgtcaga ctgacacccg caagagccag aatctcgcta cggatccctg aaaaaaaacg
     2821 ggacgcccgt ttcccggcat cttccattcg acgggccgtt ttatcagcat cttttccggt
     2881 tttttccagt gattcactgg ttttcttctg tccctgactg aatgtgtcag cgacaccttc
     2941 catcaccgtc gtgagccggt taagggcagc atgtactgcc tgttccccgg cagtaaagtc
     3001 cctgtcatca atcccgaggg acagaacaag ctcatcaagt acctgcgcca tttctgttct
     3061 cctgtatcac ctgttcgtta tgggcatcca catgaattat ttccagcaga tcccataagt
     3121 cctgcacacc aaggacagaa tccagttcag cttttgaagc cttaccggag gagataacgg
     3181 tcgcaatggt acggggaaca ttaacgtaat ccaccacccc gaacggtctg tcggggtcga
     3241 gataacgcgg ggggatatct agctgacgac ggtagcgaaa aaatccacat gcagcttaaa
     3301 gacctccgca cgcagattaa gacgggtaat gatttcttca atatcctctt caacaagcgg
     3361 tctccggatg ctgcgttttt tcggatcggg aacaaactgc acacagtcca t
//
LOCUS       DAJVUJ010000257         3328 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.260, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000257 DAJVUJ010000000
VERSION     DAJVUJ010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3328
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.260"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(1..2652)
                     /locus_tag="OQQ48_004116"
                     /pseudo
     CDS             complement(1..2652)
                     /locus_tag="OQQ48_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415720.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT          825 a          780 c          772 g          951 t
ORIGIN      
        1 acatagacat tcagctggct gttgcctgcg gtgatgtcat accccagtat catgctggca
       61 cgccccagca gcgattcgta gtgattcaga tgtatattga ggccattact cgccttcata
      121 gccatctcat tctggtggct gtatgtaagc tgggtttgcg gctctatata gaacccataa
      181 ccagtagggg acaggttgaa cctctgcccg gcttctaggg agatgctcat tccattcgca
      241 gtgccgttgg cgttaacgcc gttgttatga ctgtccagta cgtggaaact atttttctgg
      301 cgcgatgctt ttataacgag atcgctgtaa aaaccgtttt gtgccatgta actggcgtac
      361 attcccatgt agtctgaacg tgcggtaccg tcgcctccgc tatagtccgg cgatgcatgt
      421 gttgagccaa tatacagacc gacatacaac ggcatttcat cagagagacg tttatcccca
      481 ccaaactgga taccgctgta acccatgtca aagccgctca gtttgccact ggcaaaggag
      541 tccaggcttc ccccataact gcgcaaccag atattaccgt ctttactctg atttcgcagg
      601 tcacccatcc gttgcatcag cgtacggttt tcaacatagt tcaataagta accgacattg
      661 agataattgc cgccagcatc tgcggtcgtg gtgggtttag gcgtgggagc gggttcaggc
      721 gtaggatcgg ggttgactat gggaggctga gcgggagctg gtgtgggttc aggattagga
      781 gtcggttccg gaactgtccc ggaagcgtaa agctcccagt tagtgccatt tttacgcaca
      841 tcgtacagat atccccccca actcaacctg agaagacgcg ctgaacgagg ccgcgccgtc
      901 agtggttttt accactgtca gaacttcatt tcccgttgtg gcctcgctgc cctggttgcg
      961 gatagccaat acgtgattac cagcactact cccgctgata ttcaataaat cccctttatt
     1021 attaacgcca ttaccctcgc caacaacatc agcacgcata ataaaggtac tgttaccgct
     1081 caggttctct acgtttaatg tggcaaatgt gccggcagtt gacccgtggc tggcaaaatc
     1141 gacagttgaa tggctcagcg ccagcgtgtc gagattagaa ttacttgtta cgttccagac
     1201 gctgttattc attgcaacgt ccagttttcc gccattgaca ttatcgctga gggaggaacc
     1261 tgtccaaacc gacccagggt gcatatccag attgattagc ccacctttgg aaaaaacgct
     1321 accgttgata agcatacgac ccgaggcgtt gatgcggctg gcggcataac catcgtcatg
     1381 ttgcgttgca atggccatct ggtcgggtgt actcatatca atgaccagat cgctcgtgag
     1441 gtcgatctgg ctgttggtca tggcataaat tcccctggct cctgccgcgc cggtgatagc
     1501 caaactgtct ccggttattc taccgccgct gagcgcccac aaacccagcg ccagactgcc
     1561 attacgatca actgtaatat cggtattttg catgttgaca acagccgttg ccgtctgggc
     1621 cgaggcacca taagaaccgc cggaaaagat gctgtttcgt tgcgctgccg tgccagaaaa
     1681 attgattgtc gcgtctgaac cactggtgac gaggccaccg ccctgcgcgg aagaaatatg
     1741 gctatctgca ccgatagtgg ttgtaccacc acgaacttcg acgccattgg ccgcggctcc
     1801 agttacatca acagtgagtg cattcgcgct aacctggcca aagctccaga ggccgtgtgc
     1861 attatcgcca ttggttttaa tggaactgtt tgttccgaga tcgacaacag agtttttctg
     1921 tacgtttatc cccatggcgc tgtagccctg aacatcgatt gtcaggtctg tagccgtaaa
     1981 acgcgcagca ccattggcgt tattgccgtt gagaccaccg atataaacac ctgtacttcc
     2041 atcggtcgtg attttacttc cgcttccaag atcgacactg gtgccatagt cattgatggt
     2101 taggcctata ccgttcgagt tttcaatggt gaattgagtg gctgttagtg ttgagctatg
     2161 cccaataatg atgccgtcat cgttactctt aatggtgctg cctgtgccta agtcagcatg
     2221 ggtatagtca ccaattaagt taatgccgat ggcagaggtt tgaccaacaa catctactgt
     2281 gagtcggtta gctgtcaggg agctgttttt gccctgaata atgattcctt ttgcatatcc
     2341 agaggcgtcg ttgacattga cggtgacatc attacccagg ttgatattgc caggcgtgtt
     2401 tcctgctggg gtcattacac catagaggtt tcctgatgga tcggcggtat ctcctgtaat
     2461 tctgtcacca tcattaatag taatattggt gccatattga cttcctgtat cagcagctat
     2521 cagcaggggg gaaaagataa ttgcattcaa accaatagcg ccaaatttta tcagttgaat
     2581 tgagggcgaa cggattttta attcagcgag acgatctgct ttggtattcc cattgtgttg
     2641 tttgatgccc ataccactcc tatatagtac ccagactatg ggtctatgga ttttgtcatt
     2701 ttgtttaaat caatgaataa tatcctctta tcatcatgat atttgtatgg tgcaatcatg
     2761 atgagcttct cagagaataa ttctctatta ccgggcaaaa tcaggaacac aggaattaat
     2821 atatatttaa catccccctt acaaaggtga gtttatatcg agttattccc taagttgtcg
     2881 ttacttattc caattcgtta ctataaatat ggcaaaaata ttacaacaaa cagcggttaa
     2941 aaaatttggt gaagattttt tgtaataatc ctaattagtt atgggaattt cgcttgatgt
     3001 atctgcataa caaaaacaat atgcgccatt tttgcattac taataagaaa aagcaaaaaa
     3061 catcgatatt catttttggt caatgggttt tctggcctat cgttgttatt gtgctactgg
     3121 ttatcatttt catcttgcca gcatattgga gcgtgatcaa ttttgatcag ctgtgaaggt
     3181 ggctgatgtg tagtgataca aaaacttacg ttatatcact gctaatgaga tatccgtcat
     3241 ctgaacactt atgtctttaa ttattctcgt ggttcactat aggcaataag cacaaaagtg
     3301 taggatgtta caagaatgat taggactc
//
LOCUS       DAJVUJ010000258         3309 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.261, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000258 DAJVUJ010000000
VERSION     DAJVUJ010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3309
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.261"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(<1..211)
                     /locus_tag="OQQ48_004117"
     CDS             complement(<1..211)
                     /locus_tag="OQQ48_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1954674.1"
                     /translation="MIRFRLYIPPVILGFVIVPLLVWPTVIVLAVLIFTLTFLAEIIF
                     SFPLLVVCISLQELQLELMVEYALFF"
     gene            complement(214..981)
                     /locus_tag="OQQ48_004118"
     CDS             complement(214..981)
                     /locus_tag="OQQ48_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1954675.1"
                     /translation="MQPSPAMQALIEQIYHTFRRYRVPQKFVVCCEYCLSQQEQKALR
                     STSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGHFDNIHVVFSLRRIN
                     LANKENWRADERAILQRFACQYMTDWVSGDEAVELQYMLEMFFRADIALAPLLDAINS
                     VPGFWSTVSLACLLNRYCEDYIRDNQDDIDNVITTQINAWARNNHPLLKERARQAIEN
                     PLKQPEPMTEYQVWEDDWIIDECLCAMYDASSESLGK"
     gene            complement(984..1694)
                     /locus_tag="OQQ48_004119"
     CDS             complement(984..1694)
                     /locus_tag="OQQ48_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1954676.1"
                     /translation="MELVNTLFASLAGTDPFTGVDITIANCKSAYWDEGIVQQLINQA
                     LDEGEKFVGADGLEGLLRYDVTLNIGLTSSKVWPGFSLDTATISRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDVTVQFTAMLNPDERVIIAKRPLKKCDSWIEDVYIFQVFKDAW
                     KFHNNNSLRGFRDKQAELKLYTRHYSVENCAEESCRDCNSCIRPSFSLSRSALIRLNA
                     ANARFVYQPFTRDQRARG"
     gene            complement(1710..3215)
                     /locus_tag="OQQ48_004120"
                     /pseudo
     CDS             complement(1710..3215)
                     /locus_tag="OQQ48_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001472437.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
BASE COUNT          933 a          794 c          725 g          857 t
ORIGIN      
        1 tgaaaaaaag tgcatattca accattaact caagttgtaa ttcctgaaga gaaatacaca
       61 caaccaggag cggaaaggag aatattattt ctgccagaaa agttaacgtg aatataagta
      121 cggctaagac aataaccgtc ggccatacca ataatggcac gataacaaaa ccgagaatta
      181 cagggggaat ataaagccgg aatctaatca tttttatttt cctagtgatt cggatgatgc
      241 gtcatacatc gcgcataaac actcatcaat tatccagtca tcttcccaga cctgatattc
      301 tgtcattggt tcgggctgtt ttagcgggtt ctcaatcgct tgccgcgctc tttctttcag
      361 taaaggatga ttatttctag cccaggcatt gatttgggtg gtaatgacgt tatcgatatc
      421 gtcctggtta tctctgatat aatcctcaca atatctgttc aataaacagg caagagaaac
      481 ggtcgaccaa aagccgggaa ctgaattaat ggcatccagc aatggcgcaa gagcgatatc
      541 ggcacggaaa aacatttcca gcatatactg taattcgaca gcttcatcgc cggataccca
      601 atcagtcata tactggcagg caaatcgctg taaaattgcc cgttcatccg cacgccagtt
      661 ttctttgttg gcaaggttga tacggcgcag agaaaaaact acgtgaatat tgtcaaaatg
      721 cccctgtgca acaaattcca gtaaacgcgg cagaaaatag cggacctcat cgctattttg
      781 cggatcaggc cctgggctac tattccaggc attgattaag gaatacggga tcgctcgtaa
      841 cgatgtactc cgtaatgcct tttgttcctg ctgactcagg caatattcac aacaaaccac
      901 aaatttctgc ggcacccgat aacgacgaaa ggtgtgataa atctgctcaa ttaatgcctg
      961 catggcgggg ctgggttgca tagttacccc ctcgcccgct gatcgcgtgt aaacggttga
     1021 tagacaaaac gagcattcgc cgcgttcagg cgaataaggg cgctacgcga aagggaaaag
     1081 ctggggcgaa tgcaactatt gcagtctcga cagctttcct ctgcgcaatt ttccactgag
     1141 taatgacgcg tgtagagttt cagttcggcc tgcttatcgc gaaatccgcg taaactgtta
     1201 ttgttatgaa atttccaggc atctttaaag acctgaaaga tatagacatc ctcaatccag
     1261 gagtcacatt ttttaagcgg gcgctttgct attatcaccc gctcatccgg atttagcatc
     1321 gccgtaaatt gtacggtgac gtcatttgtc gtgttcaggt cacattgaac atctggtaca
     1381 tcactgatat aggggtcaaa tccaaaatca gcaccacagg cacaaagacg gctaatggtg
     1441 gcggtatcga gggaaaatcc cggccatact ttgctggagg ttaagccaat atttaatgtg
     1501 acatcatatc gaagcaatcc ctcaagaccg tcagcaccga cgaacttttc cccttcatcc
     1561 agggcctggt taataagttg ctgaacaatg ccttcatccc agtatgccga tttgcagttg
     1621 gcaatcgtaa tatccacgcc tgtgaaggga tcagtaccgg caaggcttgc aaataatgta
     1681 ttaactaatt ccatttttat tatccagctt taaggtagcg tcttatacat cgggacataa
     1741 cctacatctt ccaccaaact ctgtccctgc ggcgttaaaa accactccaa caatttttgt
     1801 gtctctgacg tcgtattttc ccgcgttacc ataaaggcat cgatgatata gggatatttg
     1861 ccgttgcgaa tgttttcggc agtcggtgca ataccgttaa tcgctagcag ttttatattt
     1921 ttatcggcgt tcatttgtgt tgcgtaataa cggaaggtgt aacctattgc gttgtttgta
     1981 ttacggtatt cggcaacgac tttaatcatt ccctccatca tacttgccac ttctgtttcc
     2041 tgcggcgaga tcatgcggac atttttcatg acctgggatt gcatcaccgt ctggctacca
     2101 gagtcttccg ggcgttgcta ggtctggatc tcctgatcgt taccgccaac agtacgccag
     2161 ttggtaattg caccactgaa gatgtcacgc acctgttgtt cagtcaggga attaaccgga
     2221 ttatccgcat tgacgatgaa aacaaacgct tcacgggcaa aaggcgtgta cagcaaagta
     2281 atgcccgatt cctcagcgcg ttttttctgc ccaccggaag gttgcgccac aaaaataata
     2341 tcagcatcac ctttaacaat cctgttatac gcatcggggg tacgagagct ctccagatat
     2401 tccctggtgt gaaaatcttc cggtataacg ctcaaagcat aaaatgcaga ggcataaatt
     2461 gggtacgccg ccgtagcacc atcgagtcgc ggccagtttt gcgtaaactg aatttgcggt
     2521 ttaccacgca gtggtgtcag ttgattattg agtttatcgg gtcgccaggc ccaggtatca
     2581 atattttcgc gcacaccgac gccgggattg agctgtttat ataaataacc ctgataacca
     2641 gcgacaaaac caagcatgag aaggataaac aggagggaat tacggcaacg tagcccggat
     2701 gtaccggtta aaggatgtct ggttggccgg ccgtacccca gcgaaaaact gatatgcgcg
     2761 ccaacgggta taatgactat ccaccagtac tcacctccga ctactgagac taacaaaacg
     2821 cccagccagg ggagcataag atcagccaac caacccgatc ccatctggtg aaattgataa
     2881 tctgccagat gcagcgcgat tatccagcca accaggatta ataaaccggg tactaaaaaa
     2941 agacattgtc gccagacggc tatttctctt ttttctggtg cactatcgcc tgcccatact
     3001 cgccccgaga aaaatagcat taatgcgccc agcgccagca taaaaatgtt gagcagaagg
     3061 taacttgttg gcgtgataag gatattacaa acttcaataa taccggcaag tccgacaccc
     3121 agcatagcaa caaccgccgc caaaaattgc gtcagtatgg ggatgccgaa aaaagtcatt
     3181 accagcgagg acaaaatcca ttttctgttt tgcattattc tttccattct ttttgaatgg
     3241 tgaaattata ctccccgagt ccccttgccc cttctggaca cttttccgaa atgatggcgg
     3301 aaaaaaacg
//
LOCUS       DAJVUJ010000259         3228 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.262, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000259 DAJVUJ010000000
VERSION     DAJVUJ010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3228
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.262"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(<1..1010)
                     /locus_tag="OQQ48_004121"
     CDS             complement(<1..1010)
                     /locus_tag="OQQ48_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCS1954677.1"
                     /translation="MKIGTVAGTNDSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTDHSEKYSMLSGMRAHILRR
                     DNNYQFIVTINSKNYGCSLDNTDVNWCSVVYLLNNMTVNDNANDVAVTESYKPIWNWK
                     ISQYNVFDIKFETIINPQFADRIYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GYLGIMTEGENFLCQLLTIEDEVLNVLLRENFNEQLDTNINTIISNLNRRDNVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINENVHVLAGNHETNC
                     NG"
     gene            complement(1272..2862)
                     /locus_tag="OQQ48_004122"
                     /pseudo
     CDS             complement(1272..2862)
                     /locus_tag="OQQ48_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
BASE COUNT         1033 a          592 c          507 g         1096 t
ORIGIN      
        1 ttaccattgc aattcgtttc atgatttcca gctaatacat gaacattttc attgatactc
       61 attatgcgtt ttaacaaatc gatgataaat tgatcgccac agatattact aaatcgatcg
      121 ccggtttggt cgcccagaaa cgtacatggc gtgactgcgt ctttattaat aactaaataa
      181 ggctgaagac tttctaaaac attatctctt ctgttcaggt tactgattat tgtattaata
      241 ttagtgtcaa gttgctcatt aaagttttcc cttagcaata cgttgagaac ttcatcttct
      301 atagtaagca actggcaaag gaaattttct ccttcagtca tgattcccaa atatcccgta
      361 gcaaaaagcg cgtacaaaac tgcaccaact gaaccatcgg tatccccaaa ataggtcggc
      421 ctggtacttg tgggatcaac aggtgaacaa ttggagaaat agattctgtc agcgaactgg
      481 gggtttataa ttgtttcgaa tttaatatca aatacattgt attgcgatat tttccagttc
      541 cagatgggtt tgtacgactc agtaactgca acatcattag cattatcatt tactgtcata
      601 ttattaagta aataaacaac actgcaccaa ttaacatcag tattatcaag agagcaaccg
      661 taatttttac tattaattgt cacaataaac tgataattat tatccctacg tagaatatga
      721 gcacgcatgc ccgatagcat actatatttt tctgaatgat ccgtttgtat ggtaaatgaa
      781 attttgtcat cttgaataga ttgttggaac cgttctattt gccaatcatc tattttttcc
      841 acaacctcac cgagcattgt cctggcaata cttgatatat catcacctat accatttgca
      901 atatacccaa acagttcttt agtgaagttg gtaacatgct cttgcaccat atcatttgtt
      961 gcgattgtcg ttgtactgtc gttagtgcct gccacagttc caattttcat cttttacctc
     1021 atattattca ccgcgaggaa tacatagtta acattttatc ttatgacata cacctcatgt
     1081 tcgatggaaa aataattata ttttgcacaa atcattcagc cttaaagcat aatatgaaat
     1141 ttcttatttc atagcagaga aagtccctaa agattgtaga ggcgtcatca gaacatgtct
     1201 ctacaatcga ttaagaaaac ctatgaaata ctggcaggat taaaaagata taccacagca
     1261 ctcatggagt tttaatgtgg ttgaaaacaa ctaagccctg ggacaccttt tttatcacca
     1321 cgttcgttag gagctatctt ccaggaattt agccccatgg tataattatc aattttttga
     1381 tgctgatcta cccctaatcc ataatggcca tatttctgtt tagttttttt gagcgtctgt
     1441 ccacatttga agatatcttc aggttttggt ctgaaagcag tagtgttatt aaaataattg
     1501 tcatatacag gtgtggatgc tatagcattg cttctaatta gatggataaa acgatcatcc
     1561 atgtgttgtt tatgtttttt attaaatata tttgctaatt cctcaggatc aattgggtct
     1621 ttcatctgat taaatggaac ctgcaaagca cctagacaat aacattcatt ttcttcattt
     1681 cttattattc cgtgatgact gcttaaaact ttcgtttctg agtcataatt acaaacatcg
     1741 agcgttttaa ttaagttata cgtatcaccg gcagagagtt tatgatttgc tagtcgtgca
     1801 gtatagttac cattgaaatt gatttcataa tttccagcca gaacgacgtc atttttattt
     1861 atacgactat ctttattact ctccatattc cgcatagaat ttagcaaagt aaggatatat
     1921 ttatcaccaa aaatagtact gaatctatcg cctgtatgat ccccaagaaa gatacaaggg
     1981 gtaatcgcat cgccttttaa aaaaacatgt tgatgcaact tattcaagat catgtctctc
     2041 gtatctaata aagtaaagag tttttccaat tttttatttt catatgcgaa tgaagccatg
     2101 tcttcgtgtt ttaataattc acataataaa ctccccccct tttccctgat acctatatgt
     2161 cctgaaacga gcaatgcata aagtacagcg ccaactcttc catctgtatc gccaaaataa
     2221 gccgtgttgg gattttgtgg gttaatcaca ccacactgtt tataaaaatc tatttttgaa
     2281 aacacagaat taacatagct agcaaactta tccttaggaa acgtctcttt agagacatcc
     2341 caacgctagt cagctttaaa attaccgcta agaagagtaa agaaattttc aggtatgata
     2401 gaaaggccat taactttata aattatgtct gatttatctc cggagaaact aataaaataa
     2461 taaccatcct tacactcaat cgtaacggta atgggagacg acaattttat tacatggtta
     2521 ttgtctggta taattgtaaa gataaacgtg tcagtagtat tatcggcgaa actattatat
     2581 tcttgaatat ctatagatgg ctcattattg taaaaccata tttttttcta tctgagaact
     2641 atattcattt accttccagc gaccatgttg caggaaatag atgatatgca ggcctaaatc
     2701 gttatctatt tgcgaatgtt tatccagctt atcaatttca actatctttg aacgatgttt
     2761 tttatcaacc ttgccatggt gttgatgatt gttaacttga tctgaatgct tatcttgatg
     2821 gcttgaagag atgtgcttaa atatattgtg aaatactttc ataaataacc cgcaatattt
     2881 ataacttgtt agtaacaaaa taaacgatac atgtctgaaa gacatattta cgacgataca
     2941 aagtaataat acttatatat ccgatgattt taagcaccga gactatccgg tcttttacta
     3001 aataatggaa aacaatccat ccctggaaga taattgaaaa aaacaacaga ataagaatta
     3061 atcttaggat aaacatttat ttatcatggc cttttgaacg ctgaaaagta gcgctctgta
     3121 aagtaaaccc cgctatagtt atgctatgca tttgtcttta atgatatctc gtcattgtat
     3181 taacaggctg aaattatcat taatgcacat ttcaatatta gcagggat
//
LOCUS       DAJVUJ010000260         3226 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.263, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000260 DAJVUJ010000000
VERSION     DAJVUJ010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3226
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.263"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..908
                     /locus_tag="OQQ48_004123"
     CDS             <1..908
                     /locus_tag="OQQ48_004123"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HCS1954678.1"
                     /translation="ANIDVAGYSHESLNYADSNPGKIKFTPGGVGRNIAQNLALLGNK
                     AWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLDNTGEMLVAINDM
                     NISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVPVFVDPVSAWKCV
                     KVRDRLNQIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLNRLVLSMGGDGVY
                     YSDISGENSWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESVRFAQGCSSMALS
                     CEYTNNPDLSIANVISLVENAERLN"
     gene            875..1834
                     /gene="psuG"
                     /locus_tag="OQQ48_004124"
     CDS             875..1834
                     /gene="psuG"
                     /locus_tag="OQQ48_004124"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HCS1954679.1"
                     /translation="MVSGERRTSELKISPELLQISPEVQDALKNKKPVVALESTIISH
                     GMPFPQNAQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEFELLGREGHNVTKVS
                     RRDLPFVVAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQEL
                     ANTNVTVVCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDS
                     ASEIARAMAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKES
                     TLFLLARVAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            1928..3177
                     /locus_tag="OQQ48_004125"
                     /pseudo
     CDS             1928..3177
                     /locus_tag="OQQ48_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
BASE COUNT          770 a          726 c          873 g          857 t
ORIGIN      
        1 cggcgaatat tgatgtcgcc ggatattcac atgaatcatt aaattatgcg gattcaaatc
       61 caggtaaaat aaaatttacg cctggtggag tcgggcgcaa tattgcacaa aacctggcgt
      121 tgctggggaa caaagcctgg ctactgagcg ccgtaggcag tgacttttat ggtcaatcgc
      181 tgctaacgca aaccaatcaa tctggcgttt atgtcgataa atgcctgatt gtgccgggag
      241 aaaatacgtc tagttattta tcattactcg ataataccgg tgaaatgctg gttgctataa
      301 atgacatgaa tattagcaac gctattacag ctgaatatct cgcacagcac cgtgaattta
      361 ttcagagggc aaaggtcatt gtcgcggact gtaatatcag tgaagaggca ctggcatgga
      421 ttctggataa tgccgccaac gtacccgtat ttgtcgatcc ggtttccgca tggaaatgtg
      481 tcaaagtgcg cgaccgtcta aatcagatcc acactctgaa gccaaaccgc cttgaagcgg
      541 aaaccctgag tgggattgcg ctgtcagggc gtgaagatgt ggcaaaagtt gctgcctggt
      601 tccatcaaca tggcctgaac cgactggtat tgagcatggg cggcgacggc gtttattaca
      661 gcgatatcag cggtgaaaat agctggtctg cgccgatcaa aaccaatgtt attaatgtta
      721 ccggagcggg cgatgccatg atggcgggac ttgcttcgtg ttgggtagac ggaatgccgt
      781 ttgccgaatc tgttcgtttc gcacagggat gttcgtcaat ggcgctctcc tgtgaataca
      841 ccaataaccc cgatttatcg attgccaacg ttatatcgtt agtggagaac gcagaacgtc
      901 tgaattaaaa atttcccctg aattattaca aatttccccg gaagtgcagg acgctttaaa
      961 aaacaaaaaa ccggttgtgg cgctggaatc gaccattatt tctcacggga tgccgttccc
     1021 acaaaatgcc cagaccgcaa ttgaagtaga agaaactatt cgtaaacagg gcgctgtacc
     1081 tgccactatc gccattattg gcggcgtgat gaaagtgggt ttaagcaaag aagaatttga
     1141 attactgggt cgtgaagggc ataacgtgac caaagttagt cgtcgcgatt taccttttgt
     1201 tgttgccgcc ggaaaaaatg gcgcaaccac tgtggcttca acgatgatta ttgcggcgct
     1261 tgccggaatt aaagtatttg ccaccggggg aattggtggt gtgcatcgcg gggcggaaca
     1321 taccttcgat atttctgccg atttgcaaga actggcaaat actaatgtca ccgttgtttg
     1381 tgccggggcg aaatctattc tcgatttagg attaaccact gagtatttag aaaccttcgg
     1441 tgtgccgtta attggctatc agactaaagc gctgcctgcg tttttctgcc gtaccagctc
     1501 gtttgacgtc agtattcgtc tcgacagcgc cagtgaaatt gcccgtgcaa tggcggtgaa
     1561 atggcaaagc gggctgaacg gtggcctcgt ggtagcgaac ccgatcccgg aacagtttgc
     1621 gatgccggag gaatctatca atgcagccat agatcaagcc gtcgccgaag ccgaagagca
     1681 gggcgttatt ggtaaagaaa gtacactgtt cctgctggcg cgcgttgctg agctgaccgg
     1741 cggtgacagc ctgaaatcca atatccagct ggtattcaac aacgccattc tggcgagtga
     1801 aattgccaaa gaataccagc gtctcgcggg ttaatttccg acgggcagaa agccgtcgcc
     1861 cgtactcatc aagcatacca aaacctggcg ctgacgccgg gtttactggc atgaagggaa
     1921 aatcattatg gatataatga gaagtgttgt ggggatggtg gtgttactgg caatagcatt
     1981 tctgttgtca gtgaataaaa agagcatcag tttgcgcacg gttggagccg cactgctgct
     2041 gcaaatcgct attggtggca tcatgctcta cttcccaccg ggaaagtggg cagtagaaca
     2101 ggcggcatta ggcgttcata aagtgatgtc ttacagtgat gccggtaggc cttcattttt
     2161 ggttcgctgg ttgggccgaa aatggatgtc ctgtttgacg gtgcgggttt tatcttcgcc
     2221 tttcgcgtac ttccggcgat tattttcgtt actgcgctca tcagtctgct gtactacatt
     2281 ggcgtgatgg ggctgctgat tcgcatcctt ggcagcattt tccagaaagc tcttaacatc
     2341 agcaaaatcg aatcttttgt cgcagtcaca actattttcc tcgggcaaaa tgagatcccg
     2401 gcgatcgtta aaccgtttat cgatcgcatg aatcgcaacg agttgtttac cgcaatttgt
     2461 agcgggatgg cgtccattgc tggttcgatg atgattggtt atgccggaat gggcgtacca
     2521 attgactacc tgttagcggc atcgctgatg gcgatcccag gtggtatttt gtttgcacgt
     2581 attcttagcc cggccaccga gccttcgcag gtcacatttg aaaatctgtc gttcagcgaa
     2641 acgccgccaa aaagctttat cgaagcggcg gcgagcggtg cgatgaccgg gctgaaaatc
     2701 gccgctggtg tagcgacggt ggtaatggca tttgtcgcaa ttattgcgct gatcaacggc
     2761 attatcggcg gaattggtgg ctggtttggt ttcgccaatg cctctctgga aagtattttt
     2821 ggctatgtgc tggcaccgct ggcgtggatc atgggtgtgg actggagtga tgccaatctt
     2881 gcgggtagcc tgattgggca gaaactggcg attaacgaat tcgtcgctta cctgagtttc
     2941 tccccatacc tgcaaacggg cggcacgctg gaagtgaaaa ccattgcgat tatctccttt
     3001 gcgctttgtg gttttgctaa ctttggttct atcggtgttg tcgttggcgc attttcggct
     3061 atttcgccaa aacgcgcgcc ggaaatcgcc cagcttggtt tacgggcgct ggcagcagca
     3121 acgctttcca acctgatgag tgcgactatt gccgggttct ttattggttt agcttgagtt
     3181 tcattgccgg atgtacgcgc ttattaagcg ttgcatccgg caatcg
//
LOCUS       DAJVUJ010000261         3208 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.264, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000261 DAJVUJ010000000
VERSION     DAJVUJ010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3208
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.264"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            381..1100
                     /locus_tag="OQQ48_004126"
     CDS             381..1100
                     /locus_tag="OQQ48_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HCS1954680.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVGHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            1111..2538
                     /locus_tag="OQQ48_004127"
     CDS             1111..2538
                     /locus_tag="OQQ48_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HCS1954681.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVWFAKNKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            2531..>3208
                     /locus_tag="OQQ48_004128"
     CDS             2531..>3208
                     /locus_tag="OQQ48_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HCS1954682.1"
                     /translation="MDNRGEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYL"
BASE COUNT          831 a          675 c          904 g          798 t
ORIGIN      
        1 attgcttcaa ttaaataaaa tcccgactat tacttgatac gtgattattg tcggtgattt
       61 tgtttgtaca ccatcagtgt atatctcaat aacccctgaa taagtagctc tgaataggta
      121 taagggatgt ggccattttt aaatggattt cttatgccaa aaatgatcgt cgatttctcc
      181 ataaaatgtg agcgatgccg aaagaaataa aattagttat tgcatttgac gtttgggtga
      241 aagattatca tttgtcatac aaatgagggg tagtatgttg ctagccaatt aaaaaagaac
      301 gccatattta ttgatgattg atcccccggt gggggggaaa catcgggcat aaatgggcat
      361 gaagtaatgg agtattagtt gtgaatgtca atttctttgt cacctgtatt ggtgacgccc
      421 tgaaatcaag aatggcacga gactccgtgc tgctactgga aaaactcggc tgtcgcgtaa
      481 atttcccgga gaaacaggga tgctgcggtc agcctgcgat caatagcggt tatatcaaag
      541 aagcgattcc agggatgaaa aatctgatcg ccgcactgga ggataacgac gatcccatta
      601 tttcaccggc tggctcttgc acctatgccg taaaaagtta cccgacgtat ctggcggatg
      661 aacctgaatg ggcatcacgt gccgaaaagg ttgccgcgcg tatgcaggat ctcacctctt
      721 ttattgttaa taaattaggg gtagtcgatg taggtgccag tttgcaaggg agagctgtgt
      781 atcacccatc ttgtagcctg gcccgtaagc tgggagtgaa ggacgagcca cttacgctgc
      841 tgaaaaatgt gcgtggactg gagctgttga cctttgctga acaggatacc tgctgcggat
      901 ttggcggcac gttctcggtc aaaatggccg aaatatccgg cgagatggtg aaagaaaagg
      961 ttgggcacct gatggaagtc cgccctgagt atttaattgg tgctgacgtg agttgcctgc
     1021 tgaacatcag tgggcgatta caacgggaag ggcagaaagt caaagtgatg catattgctg
     1081 aagtgttgat gagccgctga ggatataaag atgtcgatca aaaccagtaa tacagatttt
     1141 aagactcgca tccgtcagca aattgaagat ccgatcatgc gcaaagcggt ggcaaacgcg
     1201 cagcagcgta ttggggcaaa tcggcaaaaa atggtcgatg aattggggca ctgggaggag
     1261 tggcgcgatc gggccgccca gatacgtgat catgttctga gtaatctcga cgcttatctg
     1321 taccagctct cagaaaaagt gacgcaaaac ggcggtcacg tctggtttgc aaaaaacaaa
     1381 gaagacgcta cccgctacat tttacaggtt gcccaacgca aaaatgcccg gaaggtggtg
     1441 aaatctaaat cgatggtgac cgaagagatt ggtgtcaatc atgtgttgca ggatgctggc
     1501 attcaggtga ttgaaaccga tctgggtgaa tatattctcc agctggatca agatccgcca
     1561 tctcatgttg tggtcccggc aattcataaa gatcgccatc agatccgtcg agtgctacac
     1621 gaacgtctgg gctatgaggg gccggaaacg cctgaagcga tgaccttatt catccggcaa
     1681 aaaatccgcg aagatttcct cagtgctgaa ataggtatta ccggctgtaa tttcgcggtg
     1741 gcagagacag gttcggtatg cctggtgacc aatgaaggta atgcgcgaat gtgtaccacg
     1801 ctgcctaaaa cgcatattgc agtgatggga atggagcgta ttgcccccac gtttgccgag
     1861 gtagatgtat tgatcaccat gctggcgcgc agtgccgttg gtgcacgttt gacgggatac
     1921 aacacctggc tgacaggacc gcgcgaagcg gggcacgttg atggtcctga agagtttcat
     1981 ctggttattg tcgataacgg gcgttctgag gtgctggcct ctgaatttcg ggatgtgctg
     2041 cgctgtattc gctgcggggc ttgtatgaat acttgtccgg catatcgcca tattggcggt
     2101 catggatatg gctctattta tccagggcca attggtgcgg tgatttctcc gctacttggc
     2161 ggctataaag attttaaaga tttaccctac gcctgctctt tatgcactgc ttgtgacagc
     2221 gtgtgtccgg tgcgtattcc gctgtcaaaa ctgattttgc gtcatcgtcg ggtgatggct
     2281 gaaaaaggga tcaccgcaaa agcagagcaa cgggcgataa aaatgttcgc ttatgccaat
     2341 agtcatccag gattgtggaa agtcgggatg atggccggcg ctcatgcggc aagctggttt
     2401 atcaatggcg gcaaaacacc actcaaattt ggcgcgatta gcgactggat ggaagcacgc
     2461 gatcttcctg aagctgacgg agagagtttc cgtagttggt ttaagaaaca tcaggcgcag
     2521 gagaaaaaga atggataatc gaggcgaatt tttgaataac gttgctcagg cactgggtcg
     2581 cccgctgcga cttgaaccgc aagcagaaga tgcgccgctt aacaactatg ctaacgagcg
     2641 gcttacccaa cttaaccaac agcagcgctg tgacgcgttt attcagtttg ccagcgatgt
     2701 tatgttgacg cgctgtgagc tgaccagcga ggcgaaggcg gcagaagctg caatacgtct
     2761 gtgtaaagag ctgggagatc agtcggtcgt gattagcggt gacacgaggc tggaggaatt
     2821 ggggattagt gaacgtttgc agcaggaatg caatgccgtt gtttgggatc cggcgaaagg
     2881 tgccgagaat atctcgcagg cagagcaggc taaagtgggt gttgtgtatg ctgaatatgg
     2941 tttaaccgaa tcgggaggcg tggttctttt ttccgccgcc gagcgcgggc gttcattgag
     3001 cctgctcccg gaatcttctc tttttatcct gcgtaaaagc acaatcctgc cgcgtgtagc
     3061 gcaactcgca gaaaaattgc atcagaaagc gcaggccggt gaacgaatgc cgtcctgcat
     3121 taacatcatt agcggcccta gttcaacggc ggatattgag cttatcaaag tcgtcggggt
     3181 tcatggcccg gtgaaagcgg tgtatctg
//
LOCUS       DAJVUJ010000262         3195 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.265, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000262 DAJVUJ010000000
VERSION     DAJVUJ010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3195
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.265"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            199..939
                     /locus_tag="OQQ48_004129"
     CDS             199..939
                     /locus_tag="OQQ48_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HCS1954683.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(910..1677)
                     /locus_tag="OQQ48_004130"
     CDS             complement(910..1677)
                     /locus_tag="OQQ48_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HCS1954684.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(1883..2461)
                     /gene="lpcA"
                     /locus_tag="OQQ48_004131"
     CDS             complement(1883..2461)
                     /gene="lpcA"
                     /locus_tag="OQQ48_004131"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HCS1954685.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            2701..>3195
                     /gene="fadE"
                     /locus_tag="OQQ48_004132"
     CDS             2701..>3195
                     /gene="fadE"
                     /locus_tag="OQQ48_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="functions in fatty acid oxidation; converts
                     acyl-CoA and FAD to FADH2 and delta2-enoyl-CoA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HCS1954686.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFF"
BASE COUNT          765 a          810 c          819 g          801 t
ORIGIN      
        1 atcttaggaa ataaatgata catgcgcttt tcggactatt gaaaactgcc cctgtgctaa
       61 atttgtaaac cctgcgccga caggcttgcg taaaactctt ttggtgataa tgtgagcctt
      121 ccaaatccag actattccga tttgggatcc attttgccgt cctggcgtgt gtaaccgttt
      181 tatcaaggaa taagcagtat gcgtaaaatc gcattaattc ttgcgatgtt gttgatcccg
      241 tgcgtttcgt ttgccggttt gctgggcagc agtagctcga ccacgccagt gagcaaagag
      301 tataagcagc agttgatggg atcccctgtc tacatccaga tcttcaagga agaacgtacg
      361 ctcgatctct acgtcaaaat gggcgagcaa tatcagctgc tcgacagtta taaaatctgt
      421 aaatattccg gcggcttagg gccaaaacag cgtcagggcg atttcaaaag cccggaaggg
      481 ttttatagcg tccagcgtaa tcagttaaaa ccagacagcc gttactacaa agcgattaat
      541 attggtttcc ccaatgccta tgaccgtgcg catggttatg aagggaaata cctgatgatc
      601 cacggcgatt gtgtttccat cggctgctac gcaatgacca atcagggtat tgatgagatc
      661 ttccagttcg ttactggtgc gctggtgttt ggtcagccga gcgtgcaagt gagtatttac
      721 ccgttccgca tgaccgacgc caatatgaag cgccataaat attccaactt taaggacttc
      781 tgggagcaac tgaagccggg ttacgactac tttgagcaaa cccgtaagcc accgaccgtt
      841 tctgtggtca atggtcgtta cgtggtcagc aagccgttaa gccacgaagt tgtgcagcca
      901 caactggcat caaactacac gctccccgag gcaaaataag cgccattcgc ctggcataat
      961 cttttgccag gtttcattgc ccgtcagcgg ctgtgtcgca atcaccgtga ccacatcatt
     1021 cggtgtggtc tgcgagctga agtcgatttc tacatcctga tccagcagcg ttgccacgcc
     1081 aaacggtgcg cggcgggtga tccagtgcag attcgtcgag caatacgcca ttacatagcg
     1141 cccatccgaa agcagcatgt tgaaaacgcc cttctgccgc agttcgtccg ccagtgaagc
     1201 gatatattta aacactgctg ccatgttgcc cggcgtgcgc gggtaacgct gcgttaattt
     1261 atgcagcagc cagcagaagg ctttttcgct gtcggtttcg cctaccgggc ggaagttgcc
     1321 ggtttccagt gatttgtagc ccgtcagttg tccgttatgg gcataagtcc agttacgccc
     1381 ccataactcg cgggtaaatg ggtgagtatt ttccaacgcc acctcgcccc gattagcctg
     1441 gcgaatatga gccaccaccg aacaggattt tatcgggtag tcctggacaa gtttggcgat
     1501 gggggaatta aagctgggtt gtggatcttt aaatgtgcga cagcctttac cttcgtaaaa
     1561 ggtaatgccc cagccatctt tatgtggccc ggttccacca ccacgctgta caagtccggt
     1621 gaaactaaag cagatatcgg tagggacgtt ggcgctcatc ccgagcaatt cgcacatggg
     1681 taacctccac aacagcggga gcgcatggcc cccggcgaag attgatttaa gaattttcag
     1741 gtcggatgcg gcgtaaacga attattcggc ctacggttcg cgtacagatt tgtaggcatg
     1801 ataagacgcg tcagcatcgc atcaggcatc agcgcacaag tgccggatgc ggcgtaaacg
     1861 ccttatccgg cctacgccag acttacttaa ccatctcttt ttcaattaac tggatcagga
     1921 tatggatcac tttaatgtga atctcctgaa tgcggtcggc ataaccaaag tgcggtacgc
     1981 gaatttcgat atccgccgtg ccagccattt tgccgccgtc tttaccggtc agggtgatca
     2041 ctttcatccc cttctcacgc gccgctgcga tcgctttgat cacgtttgca gagttaccgg
     2101 aggtggagat ccccagcagt acatcgcctt cgcgacctac cgcttcaacg tagcgggaga
     2161 aaatatcatt gaaaccgaaa tcattaccga cgcaggaaat atgactaacg tcagaaatag
     2221 caatcgccgg gtagcccgga cggttttcac ggtagcgacc ggtcaactct tcggcaaagt
     2281 gcatagcgtc gcaatgggaa ccgccgttgc cgcaggaaag cactttgcca ccggctttaa
     2341 agctgtctgc taacaggacc gccgcgcgct gaatggcgtg aatattggcg tcatctttta
     2401 aaaagttagc cagcgtttcc gccgcttcgt tcagttcgtt acgaataaga tcttggtaca
     2461 tgaggatatc cttcagcata aatgtaatag acaaaatgca gtgtaccgga taccgccaaa
     2521 agcgagaagt acgggcaggt gctatgacca ggactttttg acctgaagtg cggataaaaa
     2581 cagcaacaat gtgagctttg ttgtaattat attgtaaata cattgctaaa tgtatttaca
     2641 tccactacaa ccatatcatc acaagtggtc agacctccta caagtaaggg gcttttcgtt
     2701 atgatgattt tgagtattct cgctacggtt gtcctgctcg gcgcgttgtt ctatcaccgc
     2761 gtgagcttat ttatcagcag tctgattttg ctcgcctgga cagccgccct cggcgttgct
     2821 ggtctgtggt cggcgtgggt actggtgcct ctggccatta tcctcgtgcc atttaacttt
     2881 gcgcctatgc gtaagtcgat gatttccgcg ccggtatttc gaggtttccg taaggtgatg
     2941 ccgccgatgt cgcgcactga gaaagaagcg attgatgcgg gcaccacctg gtgggagggc
     3001 gacttgttcc agggcaagcc ggactggaaa aagctgcata actatccgca gccgcgcctg
     3061 accgccgaag agcaagcgtt tctcgacggc ccggtagaag aagcctgccg gatggcgaat
     3121 gatttccaga ttactcatga gctggcggat ctgccgccgg agttgtgggc gtaccttaaa
     3181 gagcatcgct tcttc
//
LOCUS       DAJVUJ010000263         3172 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.266, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000263 DAJVUJ010000000
VERSION     DAJVUJ010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3172
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.266"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            345..504
                     /locus_tag="OQQ48_004133"
                     /pseudo
     CDS             345..504
                     /locus_tag="OQQ48_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066419.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(722..2812)
                     /gene="iha"
                     /locus_tag="OQQ48_004134"
     CDS             complement(722..2812)
                     /gene="iha"
                     /locus_tag="OQQ48_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HCS1954687.1"
                     /translation="MRITTLASVVIPCLGFSASSIAAAEDVMIVSASGYEKKLTNAAA
                     SVSVISQEELQSSQYHDLAEALRSVEGVDVESGTGKTGGLEISIRGMPASYTLILIDG
                     VRQGGSSDVTPNGFSAMNTGFMPPLAAIERIEVIRGPMSTLYGSDAMGGVVNIITRKN
                     ADKWLSSVNAGLNLQESNKWGNSSQFNFWSSGPLVDDSVSLQVRGSTQQRQGSSVTSL
                     SDTAATRIPYPTESQNYNLGARLDWKASEQDVLWFDMDTTRQRYDNRDGQLGSLTGGY
                     DRTLRYERNKISAGYDHTFTFGTWKSYLNWNETENKGRELVRSVLKRDKWGLAGQPRE
                     LKESNLILNSLLLTPLGESHLVTVGGEFQSSSMKDGVVLASTGETFRQKSWSVFAEDE
                     WHLTDALALTAGSRYEHHEQFGGHFSPRAYLVWDVADAWTLKGGVTTGYKAPRMGQLH
                     KGISGVSGQGKTNLLGNPDLKPEESVSYEAGVYYDNPAGLNANVTGFMTDFSNKIVSY
                     SINDNTNSYVNSGKARLHGVEFAGTLPLWSEDVTLSLNYTWTRSEQRDGDNKGAPLSY
                     TPEHMVNAKLNWQITEEVASWLGARYRGKTPRFTQNYSSLSAVQKKVYDEKGEYLKAW
                     TVVDAGLSWKMTDALTLNAAVNNLLNKDYSDVSLYSAGKSTLYAGDYFQTGSSTTGYV
                     IPERNYWMSLNYQF"
BASE COUNT          795 a          837 c          732 g          808 t
ORIGIN      
        1 gacctctcag taaatatcaa tacggttctg gcgagccgct tacgtgcagc agtcttatcc
       61 ggcagtttaa aggggaggac ggagaccaat gaggcactgg agcagattca gtgcagaggt
      121 gacctgcttt atcaaactta attgtgacaa aatggcgatt gagaaggtgc aattgactta
      181 tccggattcg gtcgtggcga ggtggtgccc ctgcattctg ctaaccgggt tatgagccag
      241 gagtcagcga tagacaaagg gacttatctg accgggcccc cggaaaaatg gcgggagaga
      301 atgatacctt cctgctgacg agaccggact ttgagatgac gagcgatagt gcggcagtga
      361 tgtacagcct gatcggtagc tgcaaaactt aacggaatcg agccggaaac gtggctacgc
      421 cacgtgatca gtgtaatcaa cacctggcct gccaaccgca tgaaagagtt gttgccctgg
      481 aacgtcactc tctctgtaaa ctaattttta ccccacgtcc ttcacggtgc gcttacactc
      541 tggacaagta tgtaaatgat aagttaaaaa taatccgtgc atttcctgtg acgatattag
      601 ttgttggtaa cgaaatctca ttagcggatc ggaaatgcac ttaacctaat acgttgccac
      661 tgttccgcca ggagttgcag tggcaacgta ttctaccgtc agtgatagcg ttttgttatt
      721 atcagaactg atagttcagc gacatccagt aatttcgctc aggtatcaca tatcctgttg
      781 ttgatgatcc cgtctggaag taatcaccgg catacagcgt actcttaccg gcactgtaca
      841 ggctcacgtc actgtaatcc ttgttgagca ggttattcac cgcagcattc agcgtcaggg
      901 catccgtcat cttccaggac agacctgcat ccaccaccgt ccaggctttc aggtattctc
      961 ctttctcatc atacactttc ttctgtacag cgctcagtga cgaataattc tgggtgaaac
     1021 gtggtgtttt cccgcgataa cgggcaccca gccatgatgc cacctcttcg gtgatctgcc
     1081 agttcagttt cgcattcacc atgtgttcag gggtataact cagcggcgca cctttgttat
     1141 caccatcacg ttgttcactt cgggtccagg tgtaattcag tgacagcgtg acatcctctg
     1201 accacagcgg caatgtgccg gcaaattcca caccgtgcaa ccgggccttt ccgctgttta
     1261 catagctatt ggtgttatca tttatggaat aagagacaat cttgttggag aagtcagtca
     1321 taaaacctgt gacattggca ttcagaccgg cggggttatc gtaatacacc ccagcctcat
     1381 aactgacgct ctcttccggc ttcaggtcgg ggttaccaag tagatttgtt tttccctgcc
     1441 cggacacacc actaatccct ttatgtagct gccccattct gggtgcctta tatcccgtgg
     1501 tcacaccgcc tttcagcgtc caggcatctg ccacatccca gaccagatat gcacgcggac
     1561 tgaagtgtcc cccgaattgc tcatgatgtt catagcggct gcccgcagtc agcgcaagtg
     1621 catccgtgag atgccactca tcctcagcaa ataccgacca gcttttctgc cggaaagttt
     1681 cacctgtgct ggcaaggaca actccgtctt tcatggacga gctctgaaac tcgcccccca
     1741 ccgtaaccag atgagattct cccagagggg taagcagtaa tgaattcagg ataaggttcg
     1801 attccttaag ctcccgcggc tgaccggcaa gcccccattt gtcgcgcttc agtacactgc
     1861 gtacgagctc acgaccttta ttttctgtct cgttccagtt cagatacgat ttccatgttc
     1921 cgaaggtgaa agtatgatca tagccagctg aaattttgtt ccgctcatag cgcagggtcc
     1981 ggtcatatcc ccccgtcaga ctccccagtt gcccatcccg gttatcataa cgctgccggg
     2041 tggtatccat atcaaaccag agcacatcct gctccgacgc cttccagtca agacgtgcac
     2101 caagattata attctgtgac tccgtgggat aaggaatacg cgtggctgct gtatcgctca
     2161 gtgatgtgac cgatgagccc tgacgctgtt gtgtgctacc gcgtacctgc aggctgacag
     2221 aatcatccac aagggggcca ctgctccaga aattaaactg gctgctgtta ccccatttgt
     2281 tgctttcctg cagattcagc cctgcattga cggaagagag ccatttgtct gcattctttc
     2341 tggtaatgat attcaccaca ccgcccatcg catcagagcc atacagtgtg gacatcggcc
     2401 ccctgataac ctcaatacgc tcaatggcgg ccagaggggg catgaacccg gtattcatgg
     2461 cagaaaaacc gttgggagtc acgtcactgc ttccgccctg acgaacacca tcaatcagta
     2521 tcagcgtgta actggctggc attcctcgga tgctgatttc cagcccaccg gttttacccg
     2581 taccactttc aacatccaca ccctctactg atctcagagc ctccgccaga tcgtggtact
     2641 ggctggactg caattcctcc tggctaatca cagaaacact ggcggctgcg ttagtcagct
     2701 ttttctcata gccggatgcc gagacaatca tcacatcctc tgcagcagct atgctgctgg
     2761 ctgaaaatcc gagacaggga atgactacgg aagccagagt ggttattcgc atattactaa
     2821 tctccgatca tgttaaccgg caagacaata cacaaacaca aacaataatg agaataattt
     2881 atatttgcct tttacaaaaa ataaagatta ttaacaaaac taaatataaa taacaactgc
     2941 ctcggcatgc cagttaacag ctatccgtat ggcgtgttcg gttttgcgaa taaaaaatgg
     3001 ttactgtaga agcggatttc caccacatgt caaccatgaa tggtcagtgg tgcgagacga
     3061 ctgggatgat ttggtcgatg acttcttccg gtgaatactg atagaaacat tgaaatattg
     3121 taagagaaag cgttcaaaat tctgtggcac gtgaaaaatt ctacagcgtc at
//
LOCUS       DAJVUJ010000264         3144 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.267, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000264 DAJVUJ010000000
VERSION     DAJVUJ010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3144
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.267"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..141
                     /locus_tag="OQQ48_004135"
     CDS             <1..141
                     /locus_tag="OQQ48_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="activator of the allDC-ylbA operon involved in
                     allantoin utilization; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HCS1954688.1"
                     /translation="MRPPSPLSLAWRKFGSGKAVEDIVTLFTQRRPEISGFLEIFGNP
                     RS"
     gene            210..1304
                     /gene="mnmH"
                     /locus_tag="OQQ48_004136"
     CDS             210..1304
                     /gene="mnmH"
                     /locus_tag="OQQ48_004136"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HCS1954689.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRIDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLHAAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKAR"
     gene            1420..1785
                     /locus_tag="OQQ48_004137"
                     /pseudo
     CDS             1420..1785
                     /locus_tag="OQQ48_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(2347..2796)
                     /locus_tag="OQQ48_004138"
     CDS             complement(2347..2796)
                     /locus_tag="OQQ48_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001348602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1954690.1"
                     /translation="MLAISSNLSKMIIFIFAIIIIVVLCVITYLYLYKDESLVSKHYI
                     NYMAIPENDGVFTWLPDFFSHVAVDISIYTNVEDDYFFSYFSLTNDDGGRFKKTLTVR
                     AREQVAKIVSKNDPDTKKVWCKYGKIPGQGDGVNLFLLVKLMLRIIL"
     gene            complement(2777..3144)
                     /locus_tag="OQQ48_004139"
                     /pseudo
     CDS             complement(2777..3144)
                     /locus_tag="OQQ48_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
BASE COUNT          841 a          726 c          712 g          865 t
ORIGIN      
        1 atgcgccctc cttcgccatt gagtctggca tggcgcaaat ttggcagcgg caaagcggta
       61 gaagatattg tgaccttgtt tacccagcgc aggccggaaa tcagcggatt tttagaaatt
      121 ttcggcaacc cacgcagtta aaaatcaagt ttccttgcgc gttggcgcgt aaaatagcgc
      181 gcaagctgat ttgaggttca cttttaacta tgcaagagag acacacggaa caggactatc
      241 gtgccctgct gattgctgat acgcccatta ttgatgttcg cgcccctatc gagtttgagc
      301 aaggcgcaat gcccgccgct atcaatctgc cgttaatgaa taacgatgaa cgcgccgccg
      361 ttggcacctg ctataaacag caagggtcag acgcggcgct ggcgctgggg cataaactgg
      421 tggcgggtga aattcgtcag cagcgcatag acgcctggcg ggcagcgtgc ctgcaaaatc
      481 cgcaaggtat tctctgctgt gcccgtggcg gtcaacgctc acatattgtg caaagctggt
      541 tgcatgcagc ggggattgat tatccgctgg tggaaggcgg ttataaggca ctgcgccaga
      601 ccgcgattca ggcgactatt gaactggcac aaaaaccgat agtgctgatt ggcggttgta
      661 ccggcagcgg taaaacgctg ttagtgcagc aacagccgaa cggtgtcgat ctggaagggc
      721 tggcgcgtca tcgcggttcg gcgtttggtc gcacgttaca accacaactt agccaggcga
      781 gttttgaaaa tctgctggct gccgaaatgc taaaaaccga cgcccgtcag gatttgcgcc
      841 tgtgggtgct ggaagatgaa agccggatga tcggttcgaa tcacctgccg gaatgtctgc
      901 gcgagcgaat gactcaggcg gcgattgcgg tggtagaaga tccgtttgag atccgtcttg
      961 aacgcctgaa cgaagagtat ttcttgcgta tgcatcatga ttttacccac gcgtacggcg
     1021 acgaacaggg ctggcaggaa tattgcgaat acctgcatca cggactttcg gctattaagc
     1081 gtcggctggg gttacagcgc tataacgaac tcgctgccca gctggatacg gcgctgacaa
     1141 cgcaactcac caccggcagc accgacggtc atctggcctg gctggtgccg ttacttaaag
     1201 aatattacga cccaatgtat cgctatcagc ttgagaaaaa agcggaaaaa gttgtctttc
     1261 gcggtgagtg ggcggaagtg gcggaatggg ttaaggcgcg gtaatgaggg acgtatttgt
     1321 attagtcgcg acttgatctg acacatggcc ttgaaaggtt gagagttacc ggttttgata
     1381 tgagtgtcga atccttatcc aaaacaagag gtaactctca tgcttcatac tgccaatccc
     1441 gtcatcaaac acaaagccgg tttgctcaat ctggctgaag aactcagcaa cgtatcaaaa
     1501 gcctgtaaaa tcatgggcgt ttcgcgtgat acgttttatc gttaccgcga actggtcgct
     1561 gaaggcggtg tggatgcgca gattaatcgt agtcgccgcg ctcctaacct taagaaccgt
     1621 accgatgagg caactgaaca ggctgttgtt gattacgccg tcgctttccc gacacatggt
     1681 cagcaccggg ccagcaacga gctgcgtaaa caggggggtt ttatctccga cagtggtgtc
     1741 cgttctgtct ggttactcca taaccttgag aatctcaaaa gacgatactg aacaaatttt
     1801 atcaggtgac gttccgtaaa aagttgtatg gagttctcga tgcagtcggc gcgactatca
     1861 gtacccccgc tgaaccctgg actatcgggc cgccgtactg gggcatgtca ccctggcgtg
     1921 ccgctggttt tccgctaatg gtttatttcc tctttctttc ccggcaagaa tttattttaa
     1981 tccggaagga ttatttattt tcagataggg actcacatta aacacgtaac attttaatta
     2041 atgcatggct gaatatgtat tacacaatgt cagatatcgt aaaagaccag tgcaattcta
     2101 tgtaaactct ctgactgttc attttatttg ttgtttcagg gtcggaactc accctcaata
     2161 aacaactgaa tacgagtaga tattcatcgt ctgagctata tggctttaca caatagccga
     2221 catgtctttt ctcagtcagg aattttactt ttcctcacta cgaagttgac gacttcgata
     2281 tgggatagac tcttaattca agcaattact ctgcacaagc atcaggcaat ccagctccaa
     2341 tatttgttat aaaataatgc gtaacattaa tttcaccaac aaaaaaaggt ttacaccatc
     2401 cccttgccct ggtatcttac catatttaca ccacactttt tttgtatctg gatcattctt
     2461 tgatacgatt ttcgccactt gttccctggc cctcactgtc aatgttttct taaacctacc
     2521 cccatcatca tttgttaggg aaaaataaga aaaaaaataa tcatcttcta catttgtgta
     2581 tattgatata tccaccgcta cgtgcgaaaa aaaatctggg agccatgtaa aaactccatc
     2641 attttctggt attgccatat agtttatgta atgtttactt acaagagatt catctttgta
     2701 taaataaaga taagtaatta cgcataaaac aacaatgatt ataatagcaa aaataaatat
     2761 tatcatcttt gatagattac ttgagatagc cagcatcttg taaagccttt atcgtttttt
     2821 tatgctctgg attaatataa tcactacatc tatctgagca atctgttgtt gatggacatg
     2881 tcaacccatg gtcatttaca gccaagtctt ttttattatc ttcaatcatt tcagaatggg
     2941 atagctttac ttttctgccg tacataccga acagatttaa tccgtaatct ctaatctctt
     3001 tttcataacc cagccctttc gccgatcggc taacaccagc gtcatttaat ttagacactc
     3061 gacaaaatgc catacaatgg aaaaattgat cagtcccacc taaatttaat ctcttcataa
     3121 gtatcagata atatatccca tgct
//
LOCUS       DAJVUJ010000265         3114 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.268, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000265 DAJVUJ010000000
VERSION     DAJVUJ010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3114
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.268"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            80..2983
                     /locus_tag="OQQ48_004140"
     rRNA            80..2983
                     /locus_tag="OQQ48_004140"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          827 a          681 c          965 g          641 t
ORIGIN      
        1 gaacaacgaa agttgttcgt gagtctctca aattttcgca acacgatgat gaatcgtaag
       61 aaacatcttc gggttgtgag gttaagcgac taagcgtaca cggtggatgc cctggcagtc
      121 agaggcgatg aaggacgtgc taatctgcga taagcgtcgg taaggtgata tgaaccatta
      181 taaccggcga tttccgaatg gggaaaccca gtgtgattcg tcacactatc attaactgaa
      241 tccataggtt aatgaggcga accgggggaa ctgaaacatc taagtacccc gaggaaaaga
      301 aatcaaccga gattccccca gtagcggcga gcgaacgggg agcagcccag agcctgaatc
      361 agtgtgtgtg ttagtggaag cgtctggaaa ggcgtgcgat acagggtgac agccccgtac
      421 acaaaaatgc acatattgtg agctcgatga gtagggcggg acacgtggta tcctgtctga
      481 atatgggggg accatcctcc aaggctaaat actcctgact gaccgatagt gaaccagtac
      541 cgtgagggaa aggcgaaaag aaccccggcg aggggagtga aaaagaacct gaaaccgtgt
      601 acgtacaagc agtgggagca cgcttaggcg tgtgactgcg taccttttgt ataatgggtc
      661 agcgacttat attctgtagc aaggttaacc gaatagggga gccgaaggga aaccgagtct
      721 taactgggcg ttaagttgca gggtatagac ccgaaacccg gtgatctagc catgggcagg
      781 ttgaaggttg ggtaacacta actggaggac cgaaccgact aatgttgaaa aattagcgga
      841 tgacttgtgg ctgggggtga aaggccaatc aaaccgggag atagctggtt ctccccgaaa
      901 gctatttagg tagcgcctcg tgaattcatc tccgggggta gagcactgtt tcggcaaggg
      961 ggtcatcccg acttaccaac ccgatgcaaa ctgcgaatac cggagaatgt tatcacggga
     1021 gacacacggc gggtgctaac gtccgtcgtg aagagggaaa caacccagac cgccagctaa
     1081 ggtcccaaag tcatggttaa gtgggaaacg atgtgggaag gcccagacag ccaggatgtt
     1141 ggcttagaag cagccatcat ttaaagaaag cgtaatagct cactggtcga gtcggcctgc
     1201 gcggaagatg taacggggct aaaccatgca ccgaagctgc ggcagcgacg ctgatgcgtt
     1261 gttgggtagg ggagcgttct gtaagcctgt gaaggtgtgc tgtgaggtat gctggaggta
     1321 tcagaagtgc gaatgctgac ataagtaacg ataaagcggg tgaaaagccc gctcgccgga
     1381 agaccaaggg ttcctgtcca acgttaatcg gggcagggtg agtcgacccc taaggcgagg
     1441 ccgaaaggcg tagtcgatgg gaaacaggtt aatattcctg tacttggtgt tactgcgaag
     1501 gggggacgga gaaggctatg ttggccgggc gacggttgtc ccggtttaag cgtgtaggct
     1561 ggttttccag gcaaatccgg aaaactaagg ctgaggcgtg atgacgaggc actacggtgc
     1621 tgaagcaaca aatgccctgc ttccaggaaa agcctctaag catcaggtaa catcaaatcg
     1681 taccccaaac cgacacaggt ggtcaggtag agaataccaa ggcgcttgag agaactcggg
     1741 tgaaggaact aggcaaaatg gtgccgtaac ttcgggagaa ggcacgctga tatgtaggtg
     1801 aagtccctcg cggatggagc tgaaatcagt cgaagatacc agctggctgc aactgtttat
     1861 taaaaacaca gcactgtgca aacacgaaag tggacgtata cggtgtgacg cctgcccggt
     1921 gccggaaggt taattgatgg ggtcagcgca agcgaagctc ttgatcgaag ccccggtaaa
     1981 cggcggccgt aactataacg gtcctaaggt agcgaaattc cttgtcgggt aagttccgac
     2041 ctgcacgaat ggcgtaatga tggccaggct gtctccaccc gagactcagt gaaattgaac
     2101 tcgctgtgaa gatgcagtgt acccgcggca agacggaaag accccgtgaa cctttactat
     2161 agcttgacac tgaacattga gccttgatgt gtaggatagg tgggaggctt tgaagtgtgg
     2221 acgccagtct gcatggagcc gaccttgaaa taccaccctt taatgtttga tgttctaacg
     2281 ttgacccgta atccgggttg cggacagtgt ctggtgggta gtttgactgg ggcggtctcc
     2341 tcctaaagag taacggagga gcacgaaggt tggctaatcc tggtcggaca tcaggaggtt
     2401 agtgcaatgg cataagccag cttgactgcg agcgtgacgg cgcgagcagg tgcgaaagca
     2461 ggtcatagtg atccggtggt tctgaatgga agggccatcg ctcaacggat aaaaggtact
     2521 ccggggataa caggctgata ccgcccaaga gttcatatcg acggcggtgt ttggcacctc
     2581 gatgtcggct catcacatcc tggggctgaa gtaggtccca agggtatggc tgttcgccat
     2641 ttaaagtggt acgcgagctg ggtttagaac gtcgtgagac agttcggtcc ctatctgccg
     2701 tgggcgctgg agaactgagg ggggctgctc ctagtacgag aggaccggag tggacgcatc
     2761 actggtgttc gggttgtcat gccaatggca ctgcccggta gctaaatgcg gaagagataa
     2821 gtgctgaaag catctaagca cgaaacttgc cccgagatga gttctccctg actccttgag
     2881 agtcctgaag gaacgttgaa gacgacgacg ttgataggcc gggtgtgtaa gcgcagcgat
     2941 gcgttgagct aaccggtact aatgaaccgt gaggcttaac cttacaacgc cgaaggtgtt
     3001 ttggcggatg agagaaagat tttcagcctg atacagatta aatcagaacg cagaagcggt
     3061 ctgataaaac agaatttgcc tggcggccgt agcgcggtgg tcccacctga cccc
//
LOCUS       DAJVUJ010000266         3086 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.269, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000266 DAJVUJ010000000
VERSION     DAJVUJ010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3086
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.269"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(<1..190)
                     /locus_tag="OQQ48_004141"
     CDS             complement(<1..190)
                     /locus_tag="OQQ48_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HCS1954691.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQ"
     gene            383..885
                     /locus_tag="OQQ48_004142"
                     /pseudo
     CDS             383..885
                     /locus_tag="OQQ48_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(931..1347)
                     /gene="rraB"
                     /locus_tag="OQQ48_004143"
     CDS             complement(931..1347)
                     /gene="rraB"
                     /locus_tag="OQQ48_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HCS1954692.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            1509..2513
                     /gene="argF"
                     /locus_tag="OQQ48_004144"
     CDS             1509..2513
                     /gene="argF"
                     /locus_tag="OQQ48_004144"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCS1954693.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIAETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
     gene            complement(2585..3067)
                     /locus_tag="OQQ48_004145"
     CDS             complement(2585..3067)
                     /locus_tag="OQQ48_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1954694.1"
                     /translation="MIWDKLVAYVNDFNLSNQEKSRLILRLFDDVKLLFNEVPVSILV
                     NDIFLKDFFMKQPDFAKWYFYQLLKKYEGEQLYLNELGYVYGNEEKTNEIVNNNPGYV
                     VEIFEEKMGNELKIRTRMMEILRDGKINICEYINKEQLEKLNPPEDLRIAIKKLGWNN
                     "
BASE COUNT          864 a          702 c          659 g          861 t
ORIGIN      
        1 gttgtccgtt aacttccata ttagcataga gataacgctt acatttcatt aatgcccatg
       61 gtaaatagat acctagcgtt ataatcgtta acaacacatt caccacacaa ataagaaaat
      121 atctctcacc tgtaccatga aaaacaaacg aatgggacgg aacatccatt tcattaataa
      181 cttgagccat aaaaattccc tgaattaaaa aatccattga tataatttat atatacagac
      241 agtaaaatgt tctgccacgt tacaatactc ttatcagtaa taggctggca aaaactttat
      301 tgctttataa gatgcttgcg cacgtaagaa gagagtggta ttaaacaaat gtataattac
      361 tttattttta ttgggttaaa ctatgaataa cattgcgccg caatcacctg taatgcgtcg
      421 cctgacgcta caggataatc ctgctattgc ccgcgtcatt cgtcaggtat ccgccgaata
      481 cggtcttacc gctgataaag gctacaccgt cgccgatccg aatcttgacg aactgtatca
      541 agtgtatagt cagccgggtc atgcatattg ggtggttgag tacgagggtg aagtggtcgg
      601 cggcggtggg attgcgccat tagcaggtag tgaatcggac atttgcgaac tgcaaaagat
      661 gtattttctt ccggctattc gcggtaaagg gctggcgaaa aaactggcct taatggcgat
      721 ggagcaggca cgagagatgg gcttcaaacg ctgctatctg gaaacgaccg ctttttaaag
      781 gaagccattg cgctttatga gcatttgggc tttgagcata tcgactatgc acttggctgc
      841 acgggccatg ttgattgtga agtgcggatg ttgcgcgaac tctaaaaaaa tgcccgctag
      901 tgtgtgctat cgggcatttt aatcaattaa ttagtggcga accccgtcat cgtcttcatc
      961 gacaaaatct tcatcgtcgc cgtcttcgcc attaggatct tcaaaataag tgccccaacc
     1021 gtcgtactca acgtcaaatt tctccgccaa tgtcatcagt tgttcaacct gggcatcgat
     1081 cagatcggca ttcaacgcgc actcgctgag gatgtcgcag caaatcacga tatcaccatc
     1141 ttcaacttcc agctcttctg gatcggtcac ttcgtaaccg agtttaaacg cttcaactgc
     1201 tgctttttcc agggtttcca gatcgtctgc ggaaagatgg tgttcgatgg tgtacagcgc
     1261 gtccggatcg ctgccatctt ccagtaattc ttcaataatc aaacgagttt cttcacgctg
     1321 ttcttccagt tgttccgggt ttgccatggc tcattcctct taatgtcctg ccgatactct
     1381 tattgtcaca taccactgac attgcctcca cctttgtgac aaagatttat gcttcagact
     1441 tgcaaatgaa taatcatcca tataaattga attttaattc attgaggcgt tagccacagg
     1501 agggatctat gtccgggttt tatcataagc atttcctgaa attactcgat ttcacgccag
     1561 ctgaactcaa cagcctgctg cagttagccg cgaagctgaa agccgataag aaaagcggca
     1621 aagaagaagc caaactcact ggtaaaaaca tcgcgctcat cttcgaaaaa gactcgaccc
     1681 gtacccgatg ctctttcgaa gttgccgcat atgaccaggg tgctcgcgtt acttatctcg
     1741 gcccaagcgg cagtcagatt ggtcataaag agtcgattaa agacactgcc cgcgtgctgg
     1801 gtcgcatgta tgacggtatt cagtatcgcg gctatggtca ggagattgcc gaaacactgg
     1861 cggaatacgc tggcgtgccg gtatggaacg gcctgaccaa tgagtttcac cccacgcagt
     1921 tgctggcgga tctcctcacc atgcaggagc atttacccgg taaagcattc aacgaaatga
     1981 cgctggtcta tgcaggtgac gcacgtaaca acatgggcaa ttcgatgctc gaagctgcgg
     2041 cgcttaccgg tctggatttg cgtctggtcg cgccacaggc atgctggccg gaagctgcgc
     2101 tggttacgga atgccgcgcc ctggcacagc aaaatggcgg gaatattacg ctgacggaag
     2161 atgtagccaa aggagttgaa ggtgctgact ttatctatac cgatgtatgg gtgtcgatgg
     2221 gggaagcaaa agagaaatgg gcagaacgga ttgcattact gcgtgattat caggtgaaca
     2281 gcaagatgat gcagttgacc ggtaacccgg aggtcaaatt cctccactgc ctgcccgcgt
     2341 ttcatgacga ccagacgacg cttggtaaga aaatggcgga agagtttggc ctacatggcg
     2401 gaatggaagt gactgatgag gtcttcgaat ctgccgccag cattgttttt gatcaggcgg
     2461 aaaaccgcat gcataccatc aaagcggtga tggtcgcgac gctcagtaaa taattgatat
     2521 cgcaataaag atgccgggaa atacaaaaac atcccggcat cacattattt tatgcagtaa
     2581 tagttcaatt attccacccc aattttttta ttgctatacg taaatcttcc ggaggattta
     2641 atttttctaa ctgttcttta ttaatatatt cacatatatt tattttccca tcccggagga
     2701 tttccatcat tcttgttcta atttttaatt cattacccat cttttcttca aaaatctcaa
     2761 cgacataacc cggattgtta tttacaattt catttgtttt ttcttcatta ccataaacat
     2821 atcctaactc attaagatac agctgttcgc cctcatactt cttaagtaat tgatagaagt
     2881 accacttcgc aaaatcaggt tgcttcatga aaaaatcctt caaaaaaata tcatttacta
     2941 aaatgctgac aggcacttca ttaaatagca acttaacatc atcaaataac cttagtatta
     3001 agcgtgattt ttcttgattg cttaagttaa aatcattgac atatgcaacc aacttatccc
     3061 aaatcaattc ctgtaattta cggcta
//
LOCUS       DAJVUJ010000267         3065 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.270, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000267 DAJVUJ010000000
VERSION     DAJVUJ010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3065
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.270"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            2..271
                     /locus_tag="OQQ48_004146"
     CDS             2..271
                     /locus_tag="OQQ48_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005063098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCS1954695.1"
                     /translation="MYKGFNEMWVEARNRAGKISGILTDFTFHDLKAKGISDYEGSSR
                     DKQLFSGHKTEGQVLIYDRKVKVSPTLDVPLPENIPSNSTCDFCH"
     gene            complement(299..388)
                     /locus_tag="OQQ48_004147"
     tRNA            complement(299..388)
                     /locus_tag="OQQ48_004147"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(352..354),aa:Ser,seq:cga)
     gene            482..1279
                     /gene="mtfA"
                     /locus_tag="OQQ48_004148"
     CDS             482..1279
                     /gene="mtfA"
                     /locus_tag="OQQ48_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HCS1954696.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            1380..1455
                     /locus_tag="OQQ48_004149"
     tRNA            1380..1455
                     /locus_tag="OQQ48_004149"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1413..1415,aa:Asn,seq:gtt)
     gene            1769..3065
                     /locus_tag="OQQ48_004150"
                     /pseudo
     CDS             1769..3065
                     /locus_tag="OQQ48_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter beta domain-containing
                     protein"
BASE COUNT          801 a          721 c          728 g          815 t
ORIGIN      
        1 tatgtacaaa ggctttaacg aaatgtgggt agaagcaaga aatcgcgcag gcaaaatttc
       61 aggtatttta accgacttca cctttcatga tctgaaggcg aaaggaattt cagactatga
      121 aggaagcagt cgggataagc aacttttctc tggtcacaaa accgaggggc aagtgctaat
      181 ctatgacagg aaggttaaag tttcaccgac acttgatgtc ccgttacctg aaaatattcc
      241 aagtaattcc acgtgtgatt tttgtcactg acttaatgat gtataagtga ttgaattttg
      301 gcggagagag ggggatttga acccccggtg gagttgcccc cactccggtt ttcgagaccg
      361 gtccgttcag ccgctccggc atctctccgt tcagatggtt gccatgatgc caggaaattt
      421 ggcattttaa cagtccctgt ccgtgcaatt ttgttcaagt gacgagtttg cgagcaaaac
      481 gatgattaag tggccctgga aagtacaaga atcagcacat caaactgccc ttccctggca
      541 ggaagcacta tcgatccccc ttttaacgtg tctgacggaa caggaacaaa gcaaattagt
      601 cactcttgcc gaacgttttt tacagcaaaa gcggcttgtt cctttacagg gctttgaact
      661 ggattcatta agaagctgcc ggatagcact tctattttgc ctacccgttc tggagttagg
      721 actggaatgg ctggatggtt ttcatgaagt cttaatttat cctgcgccat ttgtggtcga
      781 tgatgaatgg gaagacgata tcggtctggt gcataaccaa cgtattgttc agtcaggtca
      841 gagctggcag caagggccta tcgttttgaa ctggttggat atacaagatt cttttgatgc
      901 ttctggtttt aacctgatta ttcatgaagt cgctcataag ctggacaccc gtaacggcga
      961 tcgcgccagc ggagttccct ttattccgtt gcgtgaggtt gctggctggg aacacgatct
     1021 tcatgctgca atgaacaaca ttcaggaaga aatcgaattg gttggcgaga atgcggcgag
     1081 cattgatgct tatgcagcca gcgatcctgc tgaatgtttt gccgtacttt ctgaatattt
     1141 ctttagcgcc ccagaacttt ttgctcctcg tttcccttca ttgtggcaac gtttctgcca
     1201 attttatcaa caagatcctt tgcagagact gcatcacgct aatgatacag actcgttttc
     1261 ggcgacgaat gttcattaat taacaacttt gcatattaat taaccaattg aaatgactta
     1321 tgaaatttag tgttgacaga caaggtaccg ctaagtaata tgcgccccgt tcacacgatt
     1381 cctctgtagt tcagtcggta gaacggcgga ctgttaatcc gtatgtcact ggttcgagtc
     1441 cagtcagagg agccaaattc taaaaattcg cttttttagc gcaatgtcac tgaccttagt
     1501 tgaacattgt tttttaacgg atagcgggtt tttaacatct taagcgccct cgacctttat
     1561 ggttgagggc gttttgctat gaacgccatc accattttcc cctcgattat aaaacttgag
     1621 ttattcagta gtctcccctc ttgcaactca cacccaaaac tgcctaacga aaagttatta
     1681 attttcaatc atattgctat cagtatttac attttttcgc tgtgctagaa agggcgcatt
     1741 tatgttagct cgttcaggga aggtaagcat ggctacgaag aagagaagtg gagaagaaat
     1801 aaatgaccga caaatattat gcgggatggg aattaaacta cgccgcttaa ctgcgggtat
     1861 ctgtctgata actcaacttg cgttccctat ggctgcggca gcacaaggtg tggtaaacgc
     1921 cgcaacccaa caaccagttc ctgcacaaat tgccattgca aatgccaata cggtgcccta
     1981 catccttgga gcgctggaat cggcccaaag cgttgccgaa cgtttcggta tttcggtggc
     2041 tgagttacgc aaactcaacc agtttcgtac gtttgctcga ggttttaata atgtccgcca
     2101 gggtgatgaa ctggatgtcc cggcacaagt tagtgaaaaa aaattaaccc cgccgccggg
     2161 taatagcagt gacaacctcg agcaacagat agccagtact tcacagcaaa tcgggtctct
     2221 gctcgccgaa gatatgaaca gcgagcaagc ggcaaatatg gcgcgtggat gggcctcttc
     2281 tcaggcttca ggcgcaatga cagactggtt aagccgcttc ggtaccgcaa gaatcacgct
     2341 gggcgtggat gaagatttta gcctgaagaa ctcccagttc gattttctcc atccgtggta
     2401 tgaaacgcct gataatctct ttttcagtca gcatactctc catcgtactg acgagcgtac
     2461 gcagattaac aacggcttag gttggcgtca tttcactccc acatggatgt caggcatcaa
     2521 cttctttttc gaccacgatc ttagccgtta ccactcccgc gccggcattg gcgcggagta
     2581 ctggcgcgac tatctaaaat taagcagtaa cggctatttg cgactgacca actggcgcag
     2641 cgcacctgaa ctggacaacg attatgaagc acgcccggcc aatggctggg atgtacgcgc
     2701 agaaagctgg ctacccgcct ggccgcacct tggcggtaaa ctggtctatg aacagtatta
     2761 tggcgatgaa gtggccctgt tcgataaaga cgatcggcaa agtaatcctc atgccataac
     2821 cgctggactt aactataccc ccttcccgct gatgaccttc agcgcggagc aacgctaggg
     2881 taaacagggc gaaaatgaca cccgttttgc cgtcgatttt acctggcaac ctggcagcgc
     2941 aatgcagaaa cagcttgacc cgaatgaagt cgctgcacgg cgtagccttg caggcagccg
     3001 ttatgatctg gtggatcgca acaacaatat cgttctggaa tatcgcaaaa aagaactggt
     3061 tcgcc
//
LOCUS       DAJVUJ010000268         2918 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.271, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000268 DAJVUJ010000000
VERSION     DAJVUJ010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.271"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..1322
                     /locus_tag="OQQ48_004151"
     CDS             <1..1322
                     /locus_tag="OQQ48_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCS1954697.1"
                     /translation="LVVVFSWVFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTM
                     GPGLAYLAAWTYWVVHIPYLAQKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLF
                     IFFMWVASRGMKSLKIVGSVAGIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFI
                     PHIDFTYITTISMLVFAVGGAEKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSL
                     AMGMMFDSRNIPDDLMTNGQYYAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSID
                     APLKVLLGDADSKYIPASLCRTNASGTPVNGYFLTLVQVAILIMLPTLGIGDMNNLYK
                     WLLNLNSVVMPLRYLWVFVAFIAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTAFA
                     CLTGIFPKMEAFTAEWTFQLALNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(1323..2231)
                     /locus_tag="OQQ48_004152"
     CDS             complement(1323..2231)
                     /locus_tag="OQQ48_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCS1954698.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINDARV
                     AARIFLMPLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKELEIAVAGNI
                     ILDDVDAELEAVLMGAGIGYLLYEQIKEYLDTGRLECVLGDWSTERPGFQIYYPNRQY
                     MSCGLRAFLDYVKTGQISQSQRHRPQ"
     gene            2331..>2918
                     /locus_tag="OQQ48_004153"
     CDS             2331..>2918
                     /locus_tag="OQQ48_004153"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H oxidoreductase"
                     /protein_id="HCS1954699.1"
                     /translation="MQSERIYLVWAHPRHDSLTAHIADAIHQRAMERKIQVTELDLYR
                     RNFNPVMTPEDEPDWKNMDKRYSPEVHQLYSELLEHDTLVVVFPLWWYSFPAMLKGYI
                     DRVWNNGLAYGDGHKLPFNKVRWVALVGGDKESFVQMGWEKNISDYLKNMCSYLGIED
                     ADVTFLCNTVVFDGEELHASYYQSLLSQVRDMVDAL"
BASE COUNT          738 a          621 c          728 g          831 t
ORIGIN      
        1 ggctggtggt tgttttttca tgggtgttta tctttgcgct ctatttcaca ccttatgcgc
       61 taattgttgg tcagttaggc tcgaccttca aagatgggaa gggcggggtc agtacctgga
      121 ttaaacacac gatgggaccc ggactggctt atctcgccgc gtggacctac tgggtggtgc
      181 atattcccta tctggcacaa aaaccccagg caattctgat tgcgctcggt tgggcgatga
      241 aaggcgacgg ttcgctaatc aaagaatatt cagtcgtagc gttacagggg ttaacgctgg
      301 tgctgtttat cttctttatg tgggttgctt cacgcggtat gaaatcgctg aaaatcgtcg
      361 gttctgtggc agggattgcg atgtttgtta tgtcgctcct gtatgtggcg atggcggtaa
      421 ccgcgcctgc gattactgaa gtgcatattg cgaccacaaa cattacctgg gaaacgttca
      481 ttcctcatat cgactttacc tacattacca ctatttcaat gctggttttc gcggttggcg
      541 gagcagagaa gatttctcct tacgttaatc aaacgcgcaa cccaggaaaa gaatttccaa
      601 aagggatgtt atgcctggcg gtgatggttg cggtttgtgc cattctgggc tcactggcga
      661 tggggatgat gtttgactcg cgtaatatcc cggatgactt aatgactaac ggtcaatatt
      721 acgcctttca gaagctgggc gagtattaca acatgggtaa tactttaatg gtgatttacg
      781 ccattgcgaa taccctggga caagtagctg cgctggtatt ctcgattgat gccccgctta
      841 aagtgctatt aggtgatgct gacagcaaat atattccagc cagtttatgt cgtaccaacg
      901 cttctggtac gcccgttaat ggctattttc tgaccctggt gcaggtggca attctgatta
      961 tgctgccgac tctcggcatc ggtgatatga acaatctcta taaatggctg ttgaatctta
     1021 actcggtagt gatgccgctg cgttatttat gggtatttgt tgcatttatt gcagtcgttc
     1081 gcctggcgca gaaatataaa ccagaatatg tctttattcg taataagcct ctggcaatga
     1141 ccgtcgggat ttggtgtttt gcctttaccg cctttgcctg tttgacgggg atcttcccga
     1201 aaatggaagc cttcactgca gagtggacct tccagttggc gctgaatgtt gcaacgccgt
     1261 ttgtgctggt aggattagga ctgattttcc cgctgctggc gcgtaaagcg aatagtaaat
     1321 aattattgtg gtcggtgacg ctgactctgg ctgatctgac ccgtttttac ataatcgaga
     1381 aatgcgcgca agccgcagga catatactgg cggtttggat aatagatctg gaagcctgga
     1441 cgctctgtgc tccagtctcc cagtacacat tctaaacgac cagtatcaag gtactccttg
     1501 atctgttcat agagcagata cccgatacca gcacccatca aaacggcctc cagttcagca
     1561 tcaacatcat ccagaatgat atttcctgcg acagcaattt ccagctcctt tgcaaattgc
     1621 cagtggaaag gtttgccgct gggataacga aaaacaacac attgatgatt aagcagatca
     1681 tgaggatggc ggggtttgcc atatcttgca aagtactctg gcgttgctgc tacacataat
     1741 ttcactggcg ggccgatcgc cacgctgatc atatcttttt cgacgatgcc actaagtcga
     1801 acgccagcgt caaagccctg ttggacgata tcgacaagag agtcgtcagt tgtgagctcg
     1861 actttaatat caggatattc acgtgtgaaa ccaaccagta aaggcattaa aaatatacgt
     1921 gcagctaccc gggcgtcatt gattttgagc gttcctgtcg gtgtcagacg aaaatcattc
     1981 atctcatcta acatgatctg aatttcgtcg aaagctgggc gcaatcgttc atagagatta
     2041 gaacctgctt ccgttaacga tacgcttcgg gttgtccgat tgaataagcg aattttaaga
     2101 cgttgttcca gtgttttaat actatggcta atggcggacg aggataagcc taactcatcg
     2161 cccgctgcac gaaagctttg attacgggcc acggcaaaga aagtggcaaa gtcagacata
     2221 ttcatccgca ttagtgaaaa tccttcatta actcatcctg aatcatcacg tttacaggat
     2281 tatgccgcct gcgtaaagtg cgctccagaa cttaacgtgg aggtaaaatt atgcagtctg
     2341 aacgtattta tttggtatgg gcccatcctc gtcatgattc attgaccgca catattgctg
     2401 atgcgatcca tcagcgggca atggagcgga aaatacaggt gacggaactc gatttatatc
     2461 ggcgtaattt caacccagtg atgacgccgg aagatgaacc agactggaag aatatggata
     2521 aacgttattc tccagaggtt catcagcttt attcagagct gcttgaacat gacacgttag
     2581 tggtggtttt tcctctctgg tggtacagct tcccggcaat gctaaaagga tatattgaca
     2641 gagtatggaa taatgggctg gcttatggag atgggcacaa attaccattc aataaagttc
     2701 gttgggtggc gctggttgga ggagacaaag aatcatttgt ccagatgggc tgggaaaaaa
     2761 atataagcga ttatttgaaa aatatgtgca gttatcttgg tattgaagat gccgatgtca
     2821 ctttcttgtg taatacagtg gtattcgatg gggaagaact tcatgcgagc tattatcagt
     2881 cgttattatc tcaggtacgg gatatggtag atgcacta
//
LOCUS       DAJVUJ010000269         2908 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.272, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000269 DAJVUJ010000000
VERSION     DAJVUJ010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.272"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(<1..923)
                     /gene="curA"
                     /locus_tag="OQQ48_004154"
     CDS             complement(<1..923)
                     /gene="curA"
                     /locus_tag="OQQ48_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HCS1954700.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSHVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGGAGGAEKCRHAIEVLGFDVCLDHHAGDFAEQLVKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDK"
     gene            1104..1622
                     /gene="mddA"
                     /locus_tag="OQQ48_004155"
     CDS             1104..1622
                     /gene="mddA"
                     /locus_tag="OQQ48_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HCS1954701.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDSGKHVMVAGIESQNQASLHLHHSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            1619..2068
                     /locus_tag="OQQ48_004156"
     CDS             1619..2068
                     /locus_tag="OQQ48_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCS1954702.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLMLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(2069..2302)
                     /locus_tag="OQQ48_004157"
     CDS             complement(2069..2302)
                     /locus_tag="OQQ48_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HCS1954703.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(2388..2561)
                     /gene="ortT"
                     /locus_tag="OQQ48_004158"
     CDS             complement(2388..2561)
                     /gene="ortT"
                     /locus_tag="OQQ48_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HCS1954704.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            2756..2851
                     /gene="yncL"
                     /locus_tag="OQQ48_004159"
     CDS             2756..2851
                     /gene="yncL"
                     /locus_tag="OQQ48_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HCS1954705.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
BASE COUNT          699 a          749 c          698 g          762 t
ORIGIN      
        1 attttatcct ctttcaccca ttgccccatc tccttctgaa actcatggat gcggtgacca
       61 taatcctgag cgataataaa cccttgcaag cgaatacgtt ttttcagcac cgttgccatc
      121 aacaggggta aacgatccgg accgggtggt agctctgtag cgttatagct gctcactaag
      181 ccacaaacgg gaatgcgcgc agatgtatta agtaatggta gcactgcatc aaatacctta
      241 ccgcccacgt tttcataata gatatcaata ccttttgggc acgctttcac cagttgttcg
      301 gcaaaatcac ccgcgtggtg atcaagacaa acatcgaagc ccagcacctc gatagcatgg
      361 cggcattttt ccgcgccacc ggcccccccc accaccctgc aacctttaag tttgccgatt
      421 tgccccaccg ttgctcccac aggacctgtc gccgcagcta ccaccaacgt ttcgccctct
      481 ttaggctgac cgatatccag cagccccata taagcggtaa agcctggcat ccccagcaca
      541 cccagcgacc acgatggatt ttgcggatga tcgccaagtt tcaccagatc atcaccactg
      601 gatatgtcat agtcctgcca tccactgtag cccagcaccc agtcgccagg ctgataatca
      661 ggatgattcg actccacaac atggctcacc gtgccgccga ccatcacgcc gccaatatca
      721 acaggcggtg aataagatgg ctcatcgctc atacgtccac gcatatacgg gtccagggac
      781 aaataaactg tgcgcagtaa cacctgtcct tcacccggtg tggcgacatc atcttcttca
      841 agacggaaat tctccggaac cggtgcgcca tgtggacgcg aggccagaac ccaacgtcga
      901 ttacgctgct tttgttgccc cataatgctc tcctatgctt ttggcatgaa tccatcaata
      961 gtagcaggct cacgggattg gttttgtttg cagccgtcac atcccctctg caacacgcaa
     1021 taatttcatc gaggaatccg ttgcttcggg ctgttttcga tagtattgat aacatcatca
     1081 tcattatttt atcgaggcca gacatgtcca tccgttttgc ccgcaaagcc gattgtgctg
     1141 ccattgcgga aatttataac cacgccgtgt tgtatacggc ggctatctgg aatgaccaaa
     1201 cggttgatgc agataaccgc attgcctggt ttgaagcgcg gaccatagca ggttatccgg
     1261 tgctggtaag tgaagaagat ggcgtagtga cgggatatgc ctcgtttggc gactggcgta
     1321 gtttcgatgg ttttcgccat accgtggaac attcggttta tgtccatccc gatcatcagg
     1381 gcaaaggtct ggggcgtaaa ttgttaagcc gattgattga tgaagcgcgg gatagcggga
     1441 agcatgtcat ggtcgccggg atcgaatcgc aaaatcaggc ctcgctgcat ctgcaccatt
     1501 cgctgggatt tgtcgtcacc gcacaaatgc cgcaggtggg cactaaattt gggcgctggc
     1561 tggatctgac atttatgcag ttgcaactcg acgaacgcac tgaaccggac gcgattggat
     1621 gaatcagtcg ctcacccttg cttttttgat tgccgccgga attggtctgg tagtccagaa
     1681 cacgctgatg gtgcgcatca cccagacctc ctctaccatt ctcatcgcca tgttgctgaa
     1741 ctcactggtg gggattgtgc tctttgtttc tattttatgg tttaaacaag gcatggcggg
     1801 gtttggcgaa ctggtgtcca gcgtgcgctg gtggacactt atacccggct tgctgggatc
     1861 gttttttgtc ttcgccagta tcagcggata tcagaatgtg ggagcggcga caactattgc
     1921 agtactggtc gccagccagc taattggcgg tctgatgctg gatatcttcc gcagtcatgg
     1981 cgtaccacta cgggcgttgt tcggcccaat ttgtggcgca attttacttg tggttggcgc
     2041 ctggttggta gccagacgct cattttaatc agaggatggt gccgcctttg gttaactgtt
     2101 cgtggcgcgc ttccatatct tctttatgct tgcgaccgtg atgagcaata gcggtgcgca
     2161 aacgctgctg ctgggtgtag cgatcttccc gacttaactc cgcgtcatcg ctcagggcga
     2221 ttaataattc gttcatatgg gcaatgacgc tctcttcaat cgcggcatcc acgcgcgcga
     2281 tgacttcatc cagatgcgac atactttctc cttaaccact tgtgaaattt atcgattcat
     2341 ccaaatgata taagtctgcg tggtgccgtc aagccagtga gtaagattta aaacagtgaa
     2401 aacaacagcg ccaccgggaa actcatcggc catgttgccc ccaccagaaa ggcgcttaag
     2461 aaacggatgc gtttcttatc gtgagaaaga aaccaggtga taagaaatgc gattgctgcc
     2521 ataaccgcat aaaaaacaag catatgttga tagagagaca tgtacgggcg tccagccaga
     2581 tgaataaaac gcgcgcaaat atgcgtcatt ttttgacgtg catcaagttt tttcaccagt
     2641 ttgagaaaaa atcatcaaac agcaacttgc ccttatccat cttcagaatg agtattattg
     2701 cggattttct taacccgtac tatacacata ggggcaatga taaaaggtgg caaaaatgaa
     2761 tgtttccagt agaactgtag tactgataaa tgtctttgct gctgttggtt tgtttactct
     2821 tatctctatg agatttggct ggtttatttg atgtagtgat tttgcacgca gcacgtactg
     2881 tcagagaaat caactctctg acagtacg
//
LOCUS       DAJVUJ010000270         2869 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.273, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000270 DAJVUJ010000000
VERSION     DAJVUJ010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.273"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(<1..1075)
                     /locus_tag="OQQ48_004160"
     CDS             complement(<1..1075)
                     /locus_tag="OQQ48_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HCS1954706.1"
                     /translation="MAFKHYDVVRAASPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGAVISATSDPEYYFVVVLAGQSNGMSYGEGLPLPGTYDRPDPRIKQLARRST
                     VTPGGAACKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQYGTVGQGLHIAKKLLPFI
                     PANAGILLVPCCRGGSAFTTGADGTYSDASGASENSTRWGVDKPLYKDLIGRTKAALE
                     KNPKNVLFAVVWMQGEFDFGGTPANHAAQFGALVDKFRADLADMAGQCVGGSAGGVPW
                     ICGDTTYFWKQKNESTYQTVYGSYKNKTEKNIHFVPFMTDENGVNVPTNKPEEDPDIR
                     GIGYYGSKWRDSSATWTSQDRASH"
     gene            complement(1565..1641)
                     /locus_tag="OQQ48_004161"
     tRNA            complement(1565..1641)
                     /locus_tag="OQQ48_004161"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1605..1607),aa:Arg,seq:tct)
     gene            complement(1655..1731)
                     /locus_tag="OQQ48_004162"
     tRNA            complement(1655..1731)
                     /locus_tag="OQQ48_004162"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1695..1697),aa:Arg,seq:tcg)
     gene            complement(1739..1814)
                     /locus_tag="OQQ48_004163"
     tRNA            complement(1739..1814)
                     /locus_tag="OQQ48_004163"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1779..1781),aa:Ile,seq:cat)
     gene            complement(1855..2820)
                     /locus_tag="OQQ48_004164"
                     /pseudo
     CDS             complement(1855..2820)
                     /locus_tag="OQQ48_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403604.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
BASE COUNT          646 a          851 c          694 g          678 t
ORIGIN      
        1 aatggctcgc cctgtcctgt gacgtccagg tggctgagct gtcacgccat ttcgaaccgt
       61 aataaccgat accccgaatg tccgggtctt cttccggttt gttcgtcggc acattcaccc
      121 cgttctcatc ggtcatgaac ggtacgaaat ggatattctt ttccgtttta tttttatagc
      181 tgccgtacac cgtctggtac gtggattcgt tcttctgctt ccagaaatac gtcgtgtccc
      241 cgcatatcca gggaacaccg ccagcagagc caccgacgca ctggcctgcc atatccgcca
      301 ggtctgcacg gaatttatca accagcgcac caaactgtgc ggcatgattt gccggcgtac
      361 cgccaaaatc aaattccccc tgcatccaca ccacggcaaa cagcacattt ttcgggttct
      421 tctccagtgc cgcttttgtt cgaccgataa ggtccttata cagcggcttg tccacacccc
      481 agcgggttga attctccgag gcaccactcg cgtcactgta tgtgccatca gctccggtgg
      541 tgaacgctga accaccacga cagcacggaa ccagcagaat gcccgcattc gccggtataa
      601 acggcagcag ttttttggcg atatgcagcc cctgccccac ggttccgtac tgcccctttg
      661 acaggtccgc tttcggatgg ttaagacggc tcatgtcctg cacatcatgc agacaatggt
      721 ccgccggaat gatgtcgtta tatttgcatg ctgcaccgcc cggtgtcacc gtactgcgac
      781 gcgccagttg cttaatacgc gggtccggac ggtcatatgt ccccggcagc ggaaggcctt
      841 caccatacga catgccgttt gactgccctg ccagaaccac aacaaagtaa tactccgggt
      901 cgctggtggc gctgattact gcaccttctg ctgcaatcgc ctgcatcaga gtataagggg
      961 ttatggccac cggactacca aacggctgcc agccctcttt cagtttatgt gtcagctttt
     1021 ccgcaagatc tgacggcgac gccgccctga caacatcata gtgtttaaaa gccatgaatc
     1081 ctcccggccg ggataatatt gtgagtaaaa tgaggagcgg gctgaagtcc ggaagttaca
     1141 ggacaatggc agaagagaga cgacagcccg caattcgaaa aagaccgcgc agttgcgcag
     1201 agtgactact atggggtatt attcgccagc tgaaatatta cttcacgttt tattgtttat
     1261 tccttgccgc ccgcgtctcc cagcgcgggc tttttttgcc cacaagaaag cccctccgga
     1321 gaggggctgg agagtggcgc tatgtgccat tgcatggtgc cgggtgcctc ccggtgaatt
     1381 cagtaccagc acctgaatcc gcgattatcc catataccta ctcgctgatt gcccctccgc
     1441 acagggggat tcaccatgcc agtttctttt aacaaactcc ccgcaaacca gacaacagtc
     1501 aaccgtctga attgtgagac atttaaaaaa aaagcccgca aaagcgagcc agggaaaata
     1561 agtgtggcgc gttgtactgg attcgaacca gtgaccgatt gcttagaagg caattgctct
     1621 gtccggctga gctaacaacg catgatgcag ataatggacc gccatcgggg acttgaaccc
     1681 cgcgcagcca gcttcgaagg ctggcgctct ttcctgatga gctaatggcg gtatgtgatg
     1741 gtggcccttg ctggatttga accagcgacc tggcgattat gagtcgctcg ctctcaccac
     1801 tgagctaaag ggccgggagc cgcataataa cgacgcgtaa ttaattcttc aatatcatcc
     1861 gttctggctg actaaatcct gtacttcccg aaccgtctgc tcaaaacgtt cagtctccag
     1921 ctcaacgcca attgcacgac gcccgagcgc cagtgccgct ttcactgtcg aacccgaccc
     1981 catgaaaaaa tctgcaatca ggtcacccgg acgactgcta gcgctgatta tctgctgcag
     2041 catttctgcc ggtttttcgc acggatgttt cccgggatag aactgcaccg gtttatgcgt
     2101 ccacacatcg gtatacggca cctgcaccgt cacgccaaaa taccgccgca gatgtttata
     2161 ttcactcagc agctccgcat actgccggtt cagtgaggta tacgtatcca ccagctggtg
     2221 gtggagcttt tccagttcac cacgccgatg cttctcttct gccacccggg caaacagcgc
     2281 ctgtaatttc agataatcgc tttcattcgg caactgccac tgactggcac tgaaccagtg
     2341 cgacaccatg tttttctttc ctgtggcatc tgcaatctgt tttgccgtta tccccagggc
     2401 agctcgcgca tcacgaaagt aagaaatcag cggggccatc acatgctgtt tcagtgcact
     2461 gcccttcgcc tcatagccat catctttcgg gcggtacggc ccctgataat gttccgcgaa
     2521 cagaatgcgc tctgtggcgg ggaaatacgc ccgcaggctt tccttgttgc atccgttcca
     2581 gcgtccggac ggcttcgctc agataatatg gttcagcaca ctgaagcgtt cacgcatcat
     2641 gatttcgata tcagatgcca ggcgatgacc acagaacagg taaagacttc cggcaggttt
     2701 cagcacccgc cagaactgcg ccagacactg ctccagccac ttcaggtaat catcgtcgcc
     2761 cttccactgg ttatcccagc cctcaggctt cactttaaag tacggcgggt ccgtgactat
     2821 caggtcaaca gaattttcgg gtaacgacca gataaattcc aggcagtcg
//
LOCUS       DAJVUJ010000271         2831 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.274, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000271 DAJVUJ010000000
VERSION     DAJVUJ010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2831
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.274"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            1..136
                     /locus_tag="OQQ48_004165"
                     /pseudo
     CDS             1..136
                     /locus_tag="OQQ48_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            386..1252
                     /gene="ospB"
                     /locus_tag="OQQ48_004166"
     CDS             386..1252
                     /gene="ospB"
                     /locus_tag="OQQ48_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspB"
                     /protein_id="HCS1954707.1"
                     /translation="MNLDGVRPYCRTVNKKNESISDIAFAHIIKRVKNSSCTHPKAAL
                     VFLGEKGFCDSNDVLSIMGQQIPRVFKNKMLYDYVFKNEKSKNDFLKMAESWLPQSEP
                     IVINNDDDALNAAAYFSVKKAKIKTVNDTDFKEYNKVYILGHGSPGSHQLGLGSELID
                     VQTIISRMKDCGILNVKDIRFTSCGSADKVAPKNFNNAPAESLSCILNSLPFFKEKES
                     LLEQIKKHLENDESLSDGLKISGYHGYGVHYGQELFPYSHYRSTSIPADPEHTVKRSS
                     QKKTFIINKELD"
     gene            1582..2322
                     /gene="phoN2"
                     /locus_tag="OQQ48_004167"
     CDS             1582..2322
                     /gene="phoN2"
                     /locus_tag="OQQ48_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAP2 family phosphatase PhoN2"
                     /protein_id="HCS1954708.1"
                     /translation="MKTKNFLLFCIATNMIFIPSANALKAEGFLTQQTSPDSLSILPP
                     PPAEDSVVFQADKAHYEFGRSLRDANRVRLASEDAYYENFGLAFSDAYGMDISRENTP
                     ILYQLLTQVLQDSHDYAVRNAKEYYKRVRPFVIYKDATCTPDKDENMAITGSYPSGHA
                     SFGWAVALILAEINPQRKAEILRRGYEFGESRVICGAHWQSDVEAGRLMGASVVAVLH
                     NTPEFTKSLSEAKKEFEELNTPTNELTP"
     gene            complement(2675..2831)
                     /locus_tag="OQQ48_004168"
                     /pseudo
     CDS             complement(2675..2831)
                     /locus_tag="OQQ48_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
BASE COUNT          926 a          515 c          526 g          864 t
ORIGIN      
        1 agcggtaaga gaagttcatc atacttggcg cagttacgcg atgtttctgc tagaggtgat
       61 gccgtaaaga acggcagaac attaccggaa caagatagcg gtctacctgc actggtatca
      121 gacccggggc ttccccggat gatctccacg gtactttagg tactgtgtct atattatgta
      181 ggtaggatca attaactttg tagttgaaat tctttcattg aaataatgaa taaaaaagac
      241 aaaccgcatt aaacaatgcc agaatataat tgcatttttg ttccgttttt ttaaatcata
      301 gtatacaaag aagcttgcat actatatgaa aatataacac cctttactat gatgtttata
      361 aaacaatata tggagtcatg taggtatgaa tttagatggt gttagaccat actgtagaac
      421 agtcaataaa aagaatgaaa gcatatcaga tattgcattt gcacatataa taaaaagggt
      481 aaaaaattca tcatgtactc acccaaaagc agcattggtt tttttaggag agaaaggttt
      541 ttgtgatagc aatgatgttc tatctattat gggacaacaa ataccaagag tatttaagaa
      601 caagatgtta tatgattatg tttttaaaaa tgaaaaaagt aaaaatgatt ttctaaaaat
      661 ggctgaatca tggctaccac agagtgaacc aatagtaata aataatgatg atgacgcatt
      721 gaatgctgct gcttattttt ctgtaaaaaa agcgaaaata aaaacagtaa acgatactga
      781 ttttaaagag tataataagg tttatattct tgggcacggt agtcctggtt ctcatcaatt
      841 aggccttggt tcggaactta ttgatgtaca aacaatcatt tcaagaatga aagactgtgg
      901 tattctaaat gtgaaagata tccgttttac ttcatgcggc tccgctgata aagtggctcc
      961 taaaaatttt aacaatgccc ctgctgaaag tctttcttgt atccttaact ctctgccttt
     1021 ttttaaggaa aaagaatctt tgctagagca gataaaaaaa caccttgaaa acgatgagtc
     1081 attgagtgat ggtctaaaaa tatccggcta tcatggatat ggagttcact atggtcaaga
     1141 gctttttccc tactcacatt atcgttcaac ttcaattcct gctgatccgg agcatacagt
     1201 aaaaagaagc tctcagaaaa agacttttat tattaataaa gaactggatt agtataaaat
     1261 ttttaaccta tagttaattg tttgcattta agatgaagtt atttttttct ttagtagaaa
     1321 agtgcttccc tatgagggaa aatcacaaat catcataata cagagacaaa acgatacgaa
     1381 caaatagata aaaaacatca ttcttttact acattatttt tcgtgaatat gaaaaataat
     1441 gttattactt taatataaga ctattttttg tttttccatc actctgttca aatttttccg
     1501 catgacttgt gttttttgta acacagctcg ttttttacag ctgaccaaaa tcatcaatta
     1561 attatgctaa ggaaataaat tatgaaaacc aaaaactttc ttcttttttg tattgctaca
     1621 aatatgattt ttatcccctc agcaaatgct ctgaaggcag aaggttttct cactcaacaa
     1681 acttcaccag acagtttgtc aatacttccg ccgcctccgg cagaggattc agtagtattt
     1741 caggctgaca aagctcatta tgaattcggc cgctcgctcc gggatgctaa tcgtgtacgt
     1801 ctcgctagcg aagatgcata ctacgagaat tttggtcttg cattttcaga tgcttatggc
     1861 atggatattt caagggaaaa taccccaatc ttatatcagt tgttaacaca agtactacag
     1921 gatagccatg attacgccgt gcgtaacgcc aaagaatatt ataaaagagt tcgtccattc
     1981 gttatttata aagacgcaac ctgtacacct gataaagatg agaacatggc tatcactggc
     2041 tcttatccct ctggtcatgc atcctttggt tgggcagtag cactgatact tgcggagatt
     2101 aatcctcaac gtaaagcgga aatacttcga cgtggatatg agtttggaga aagtcgggtc
     2161 atctgcggtg cgcattggca aagcgatgta gaggctgggc gtttaatggg agcatcggtt
     2221 gttgcagtac ttcataatac acctgaattt accaaaagcc ttagcgaagc caaaaaagag
     2281 tttgaagaat taaatactcc taccaatgaa ctgaccccat aaagctggac agcctgtatc
     2341 aggctatgga gggcccatag acaaatctac cctatatgag cataggagga gtctatgggc
     2401 acaccacgtt ttacccctga atttaaggga ttactggaaa ggctgggaca tatccctccg
     2461 gcagaagcag aaaaagctta ttatgctgcc atcggaaacg atgatctggc aacctgagtt
     2521 cacagataaa acattctcta ggaaactcgg ggcggttccg ttcaccacat gcaatgtggt
     2581 gttgcagggg aacggtctgc accatcccct atgtcgatca atataacaga aatgacaact
     2641 tcagattcag ggcacaacct aaatatattt taggtcacct ctcaaatcgt ttgcctgata
     2701 ccgctccatt ttttaataaa aaattcaatc atttttgaat ctttgctttt cattgcattg
     2761 tcaagcatag tgtccccact atttgctttt tgaaattttt tgtttacatc aaccaatcca
     2821 ttattgataa a
//
LOCUS       DAJVUJ010000272         2827 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.275, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000272 DAJVUJ010000000
VERSION     DAJVUJ010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2827
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.275"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(117..1571)
                     /gene="ospC2"
                     /locus_tag="OQQ48_004169"
     CDS             complement(117..1571)
                     /gene="ospC2"
                     /locus_tag="OQQ48_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HCS1954709.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKINPNKDTKALQKNISCVTNSSS
                     SGISEKHLDHCADTVKSFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSL
                     EHLDKVSRHYLSEIIQKTHPLSSDERHLLSIIINSDFNFRHQSNANLSNNTLNIKSFD
                     KIKSENIQTYKNTFSEDIEEIANHDFVFFGVEISNHQETLPLNKTHHTVDFGANAYII
                     DHDSPYGYMTLTDHFDNAIPPVFYHEHQSFFLDNFKEVVDEVSRYVHGNQGKTDVPIF
                     NTKDMRLGIGLHLIDFIRKSKDQRFREFCYNKNIDPVSLDRIINFVFQLEYHIPRMLS
                     TDNFKKIRLRDISLEDAIKASNYEEINNKVTDKKMAHQALAYSLGNAKSDMALYLLSK
                     FNFTKQDIAEMEKMNNNMYCELYDVEYLLSEDSANYKVLEYFINNGLVDVNKRFQKAN
                     SGDTMLDNAMKSKDSKTIDFLLRNGAVSGKRFGR"
     gene            1996..>2827
                     /locus_tag="OQQ48_004170"
     CDS             join(1996..2338,2338..>2827)
                     /locus_tag="OQQ48_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HCS1954710.1"
                     /translation="MARYDLPDEAWTIIKPLLPPEPTTPRAGRPWAEHRKIINGMFWV
                     LCSGAPWRDLPERYGAWKTVYNRFNRWSKSGVINIIFNRLLSLLDANGFIDWSATALD
                     GSNIRALKCAAGGSKKHPDIDGDNGPGRSRGGFGTKIHLATDGSGLPLNIVLSPGQAH
                     ESQFAQRLLDGIGVQRQNGSMKRRGHAVLADKAYSGRALRNELKNNGIKAVIPRKSNE
                     KMASDGRAQLDRDAYCNRNVVERCFGRLKEYRRIATRYDKTARNYLAMVKLGCIRLFY
                     QR"
BASE COUNT          848 a          555 c          537 g          887 t
ORIGIN      
        1 cgttcaccac atgcaatgtg ttgttgcagg ggaacggtct gcaccatccc ctatatcgat
       61 caatataaca aaaatgacaa cgtcagattc agggcacaac ctaaatatat tttaggtcac
      121 ctcccaaatc gtttacctga taccgctcca tttcttaata aaaaatcaat tgtttttgaa
      181 tctttgcttt tcattgcatt gtcaagcata gtgtccccac tatttgcttt ttgaaatctt
      241 ttgtttacat caaccaatcc attattaata aaatattcta gcactttata gttggcacta
      301 tcttcactaa gtaaatactc aacatcatac agctcacaat acatattgtt gttcattttc
      361 tccatctctg cgatatcttg ttttgtaaaa ttaaatttag atagtaaata aagtgccata
      421 tcagattttg cattgcctag agaataagca agagcttgat gagccatttt tttatcagtc
      481 accttattgt taatttcttc ataattggat gctttaatgg catcctccaa agatatatct
      541 ctgagtctta tctttttgaa gttatctgtg cttagcattc ttggtatatg atactcaagc
      601 tgaaacacaa agtttataat tctatccaga ctaacaggat caatgttttt attataacaa
      661 aactctcgga atctttgatc tttactcttt cttataaaat caataagatg caacccaatt
      721 ccaagcctca tatctttagt attaaatatt ggcacatcag tttttccctg attaccatgc
      781 acatacctgc taacttcatc cacaacctct ttaaagttat ctaagaaaaa tgattggtgc
      841 tcatggtaaa aaacaggtgg aatagcatta tcaaagtggt cggttaatgt catatatcca
      901 tatggagagt catgatcaat gatatacgca ttagcaccaa aatccacagt atgatgtgtt
      961 ttatttagtg ggagtgtttc ttgatgattg gaaatttcaa ccccaaaaaa aacaaaatcg
     1021 tggttagcta tttcttcaat atcctcagag aatgtgtttt tatatgtttg tatattctca
     1081 gacttaattt tatcaaagga cttaatatta agagtattat ttgacaggtt agcgttactt
     1141 tgatgtctga agttaaaatc agaattaata ataatagaga gcaagtgacg ttcatccgaa
     1201 gaaagaggat gagttttttg tattatttca gaaagatagt gtctggacac cttatcgagg
     1261 tgctcaagtg accgaaatga tgaccgtgct ccagatggcg cttcaataga aagatttaat
     1321 gatttgaaac tggtcccttg agagaacatt ttactataac tttgcgctgc tatagacttc
     1381 cttaaaaagc ttttcacagt atcagcacag tgatctaaat gtttttcact tatgccagaa
     1441 gaggatgagt ttgttacgca tgatatgttt ttttgtaatg cttttgtatc tttgttcgga
     1501 tttatttttc catcaacaag atcaatattg ttctgaacat taatatgatt tactgcttca
     1561 ggtattttca taaaacatcc cccattatgt gcgtttttat ctgcagttat ttagaataat
     1621 attatctaac ttaatatttt cttgtatgta ttataacgaa catattttaa tatgttgtac
     1681 gattttcact ctgaaatccc ctgagtttaa tcacacttcc tttactgaaa ggaattattg
     1741 caacagtatt atttgttttt ggatatatat cttgcgcctt gttatgtagt taatgctcag
     1801 aatatgatag ataaaataga gacgcagaac agggcatgct cataacatga tgataacata
     1861 aaaaattagt aacaataaat gaaagaatca tgggcgctca acaaaaagag agctaaatag
     1921 ctgcgcggaa tagtaggctg tgtcccttaa tcatctgagt tatagtaact gtctgtttac
     1981 acaacatatt gaactatggc tcgctacgac cttcccgatg aggcatggac catcatcaag
     2041 cccctgttac ctcctgaacc caccacacca cgggccggac gcccatgggc tgagcaccgt
     2101 aaaatcatca atggcatgtt ctgggtgtta tgttccggtg caccatggcg cgatttaccc
     2161 gaacgatatg gggcatggaa aactgtttat aaccgcttta accgatggtc aaagtcaggc
     2221 gtgattaata ttattttcaa caggttgctt tcgctacttg atgcaaacgg ctttattgac
     2281 tggtctgcca ccgcgctgga tggcagtaat atccgggcgc tgaaatgtgc cgccggtgct
     2341 caaaaaaaca tcccgatatc gacggagata atggcccggg tcgctctcgc ggcggttttg
     2401 gcaccaaaat ccatctggca acagacggaa gtggcctccc gttaaatatc gtgctaagcc
     2461 ccggacaagc tcatgaaagc cagttcgcgc aacgacttct ggacgggatt ggcgttcagc
     2521 gtcagaacgg cagcatgaaa cgccgtggac atgcggtact ggctgataaa gcgtattcag
     2581 ggcgcgcatt gcgcaacgag ctgaaaaata acggtataaa ggcagtaatc ccccgaaagt
     2641 caaatgagaa aatggcatcg gatggacgtg cacagcttga tcgtgatgcg tactgcaatc
     2701 gtaatgtcgt tgaacggtgc tttgggcggc tgaaagaata tcgccgcatc gccacgcgtt
     2761 acgacaaaac ggcgagaaat tacctggcga tggtgaaact gggctgcatc cgactctttt
     2821 atcaacg
//
LOCUS       DAJVUJ010000273         2747 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.276, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000273 DAJVUJ010000000
VERSION     DAJVUJ010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.276"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..2188
                     /locus_tag="OQQ48_004171"
     CDS             <1..2188
                     /locus_tag="OQQ48_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HCS1954711.1"
                     /translation="YEKGGKDGITMTFIGPSPSVQDKSASTDNTNINGEDMTEIEESM
                     LLPVSGQELPIRWLAQHGSEKPVTHVSRDELQALHIARAEELPAVTALAISHKTSLLD
                     SLEIRDLHKLVRDTDKVFPNPGNSDLGLITAFFEAYLDADYTVRGLLTKEWMKGNRVS
                     RITRTASGANAGGGNKTDRNPNLVHTLDTLDVEIAAATLPMDFNIYEIPGSVYRRAKE
                     VVLKKESPFKEWSAALRAPPGILDYSRAAIFALIRSAHPEFYHYPGRLQGYINAYLTE
                     TDHENPSKETLTATRHTPEKDILEEINREVVTERETEEEKPQPSDAMAGEQATTETME
                     PDTTEHCQNAQSLDAQSQVSSANQVKVTADEVNKIMQAANISQPDADKLLAVSRGEFV
                     EGISDPNDPKWVKGIQTRDSVNQNQHESERNDQKAEQNSPNALQNEPETKQSEPVAQQ
                     EPEKVCTACGQSGGGNYPDCGAVMDDATYQKTFDEENQVEVQENDPEEMEGAEHPHKE
                     NAGSAQDHASDSKTGETADPLITVNGHHEITSTSRTCDHLMIDLETMGKNPDAPIISI
                     GAIFFDPQTGDMGPEFSKTIDLETAGGVIDRDTIKWWLKQSREAQSAIMTDEIPLDDA
                     LLQLREFIDENSGEFFVQVWGNGANFDNTILRRSYERQGIPCPWRYYNDRDVRTIVEL
                     GKAIDFDARTAIPFEGERHNALDDARYQAKYVSAIWQKLIPSQADF"
     gene            2247..2450
                     /locus_tag="OQQ48_004172"
     CDS             2247..2450
                     /locus_tag="OQQ48_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HCS1954712.1"
                     /translation="MSSLFLTEDELLILTGCKYASHQRKWLMENELPFYTNRSGKPIV
                     SRDLFTCNKTLPPREVEPNFGAI"
BASE COUNT          841 a          663 c          651 g          592 t
ORIGIN      
        1 ctatgaaaaa ggcgggaaag atggcatcac aatgacattt atcggccctt ccccctctgt
       61 tcaggacaaa tcagccagca ctgataatac caacatcaac ggcgaagaca tgactgagat
      121 tgaggagagc atgcttctac ctgtctccgg tcaggaactg cccattcgtt ggcttgctca
      181 acacggcagc gaaaaaccag taacgcacgt ttcacgcgac gaactccagg cattacacat
      241 tgcacgggct gaagaactac cggctgttac tgccctggct atttcgcata aaaccagtct
      301 gctcgactcg ctggagattc gcgacctcca caaactggtt cgtgacactg acaaagtttt
      361 ccctaatcct ggtaattcag acctgggact aataactgct tttttcgaag catacttgga
      421 cgctgactac actgttcggg gcctgctgac aaaagagtgg atgaaaggaa atcgtgtttc
      481 acgcatcacc cgcacggctt ccggtgctaa tgctggtggt gggaacaaaa ccgatcgcaa
      541 tccgaattta gtacacaccc tcgacacact ggatgtggag attgcagcag ccacacttcc
      601 gatggatttt aatatttatg aaattccggg cagcgtttat cgtcgcgcaa aagaagtagt
      661 cctgaaaaaa gaaagtccgt tcaaagaatg gtccgcagca cttcgtgcac ccccgggtat
      721 tctggactat tcccgcgccg ctatttttgc acttatccga agcgcacacc ctgaatttta
      781 tcactacccg ggacgccttc aggggtatat caacgcctat ttgacggaaa ctgatcacga
      841 gaaccccagc aaggaaactc tcacagctac ccggcatacg ccggaaaaag atatcctgga
      901 agaaattaac cgcgaggtgg ttactgagcg tgaaacagaa gaagaaaaac cacaaccatc
      961 tgacgcaatg gcaggtgaac aggcaacaac tgaaacaatg gaaccggata caactgaaca
     1021 ttgccagaac gcgcagtcgc tggatgctca gtcgcaggtg agttccgcta accaagtaaa
     1081 agtcaccgct gacgaagtaa acaaaattat gcaggcagcc aatatcagcc agcctgacgc
     1141 cgataagtta cttgctgtat cgcgtggtga atttgttgag gggattagcg accctaatga
     1201 tccgaaatgg gtcaagggga tccagactcg cgattctgtg aaccagaacc agcatgaatc
     1261 ggaacggaac gaccaaaaag cggaacaaaa cagcccaaat gcgttacaaa acgagccaga
     1321 aacgaaacaa tccgaaccag tagcgcaaca ggaaccggaa aaagtctgca ccgcctgcgg
     1381 tcagagcggt ggcggcaact accctgattg tggcgcggtg atggacgacg caacatacca
     1441 gaaaacattc gatgaagaga atcaggttga agttcaggaa aatgatccgg aggaaatgga
     1501 aggcgctgaa catccacaca aggagaatgc tggcagcgct caggaccacg ccagcgatag
     1561 taaaactggc gagacggcag atcccttaat tacggtgaat ggtcatcacg aaatcacatc
     1621 caccagcagg acgtgtgacc atctaatgat cgaccttgaa accatgggaa aaaatcctga
     1681 tgccccgatc atctcaatag gtgcaatatt tttcgatccg caaaccggag atatgggacc
     1741 cgaatttagt aagactatcg atctggaaac tgctggcgga gtcattgatc gggacaccat
     1801 taaatggtgg cttaagcaat cacgcgaagc gcaatctgcc attatgaccg atgaaatccc
     1861 gttagatgat gcactgttac aattgcggga atttatcgac gaaaactccg gtgaattttt
     1921 tgttcaggtc tggggaaatg gagccaactt cgacaacacg attttgcgcc gttcatacga
     1981 acggcagggg atcccctgcc cgtggcgtta ctacaacgat cgcgatgtac gcacaatcgt
     2041 tgagctgggg aaagccatag acttcgatgc cagaacggct attccattcg aaggtgagcg
     2101 ccataatgca cttgatgacg ctcgttacca ggcaaaatac gtttcagcta tctggcaaaa
     2161 actgatcccg agtcaggctg atttttaatg ttcaacccta attgccgcta accgtatata
     2221 gttagcggcg gttatgagat atagctatga gcagcttatt tttaaccgaa gatgaattgc
     2281 taatattaac gggctgcaaa tatgcaagcc accagcgaaa atggttaatg gaaaacgagc
     2341 ttccgttcta taccaatcgt agtggcaaac cgattgtcag ccgggatcta tttacctgca
     2401 ataaaacttt accaccacgc gaggtagagc cgaattttgg tgcgatctga tgggaagacg
     2461 aaggaaaaat cctgagcacg aaaaattacc tccaaatgta tacccaaata aatatagtta
     2521 tgtatggaaa ccaacatcca gcgaatctgt aacactaacc gccatcaagg atggtttagc
     2581 tgctttatgg aaaaagtatg aggaaactgt aaataatcgc gatcgtgcaa tgacattcgg
     2641 tcgcttgtgg gaaaaattcc tcgccagcgc ctattacagt gacctcagtc caagaacaca
     2701 aaaagattat ctgcaacatc aaaaaagttg cttgccgtat tcggtaa
//
LOCUS       DAJVUJ010000274         2740 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.277, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000274 DAJVUJ010000000
VERSION     DAJVUJ010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2740
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.277"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..172
                     /locus_tag="OQQ48_004173"
     CDS             <1..172
                     /locus_tag="OQQ48_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="acts in anaerobic beta-oxidation of fatty acids;
                     possible ligase/synthase involved in anaerobic growth on
                     fatty acids; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cyclohexanecarboxylate-CoA ligase"
                     /protein_id="HCS1954713.1"
                     /translation="EVVAFFSRKRVAKYKYPEHIVVIEKLPRTASGKIQKFLLRKDIM
                     RRLTQDVCEEIE"
     gene            complement(229..2607)
                     /gene="ppsA"
                     /locus_tag="OQQ48_004174"
     CDS             complement(229..2607)
                     /gene="ppsA"
                     /locus_tag="OQQ48_004174"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HCS1954714.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNGRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
BASE COUNT          653 a          761 c          683 g          643 t
ORIGIN      
        1 agaggtggtg gcttttttta gccgtaaacg ggtcgcaaaa tataaatatc ctgaacatat
       61 cgtggtaatc gaaaaactac cgcgcactgc ctcaggtaaa atacaaaagt ttttgttaag
      121 aaaagatatt atgcggcgat taacgcagga tgtctgtgaa gagattgaat aagtttcatc
      181 ttcggggatc acataaaccc ggcgacaaaa cgccgccggg tatttatttt atttcttcag
      241 ttcagccagg cttaaccagg tttgcaccac ggtgtccgga ttcagagaca ggctatcgat
      301 cccctcttcc atcaaccatg cggcaaaatc ttcgtggtcg gacggaccct gaccgcaaat
      361 cccgacatat ttgccctgtt tcttcgcggc acggatagcc atcgacagca atgctttcac
      421 cgcatcgttg cgctcatcga acagttcaga caccacgccg gagtcacgat ccagaccgag
      481 cgccaactgc gtcatgtcgt ttgagccaat tgagaagccg tcgaaatatt cgaggaactg
      541 ctcggccagc aaggcgttgg acgggatttc acacatcatg atgattttca gcccgttctc
      601 accacgcttc agcccctgac gcgccagttc ttcaaccacc gctttcgcct ggtctacggt
      661 acgcacgaac gggatcatga tctcaacgtt ggtcagcccc atgtcgttgc gcacacgttt
      721 cactgcttca cactccagcg cgaagcagtc gcggaagctg tcggaaacat agcggcccgc
      781 gccacggaag ccgagcatcg ggttctcttc atctggctcg taacgctcac caccgaccag
      841 gttggcatat tcgttcgatt taaaatcaga gagacggaca atgacgcgct tcggataaaa
      901 cgcggcaccc agcgtcgcga tcccttcagt cagacgacca acgtaaaatt cacgcggaga
      961 atcaaaacct ttcatcatct cgcggatttc gttttgcaac tgcggttcct gatcgtcaaa
     1021 ctcaagcagt gcgcgtgggt ggacgccaat catacggttg atgataaatt ccagacgcgc
     1081 aagtcccacg ccttcgttcg gcagacaggc gaagtcgaaa gctcgatccg ggttaccgac
     1141 gttcatcatc actttcaacg gcagatccgg catggtttct acgctggagc ttttcacgct
     1201 aaattccagc aactccgcat agacgtaacc ggtatcacct tcggcacaag aaacagtgac
     1261 gttctcaccg tctttcatcc gttctgttgc atcaccacag cccacgaccg ccggaatacc
     1321 cagttcacga gcgatgatcg ccgcgtgaca ggtacgaccg ccacggttgg tgactatggc
     1381 agatgctttc ttcatgatcg gttcccagtc cgggtcggtc atgtcagtga ccagcacgtc
     1441 accaggttcg atgcggttca tttcgctgat gtcatggatg actttcaccg gacccgcacc
     1501 gatgcgatga ccgatagcac ggccttcggc gataatctta ccctgtgaat gcagcgtata
     1561 acgctccatg acctgaccgc gtgagcgcac ggtttccgga cgcgcctgca caatgaacag
     1621 tttgccggtg tggccatctt tcgcccactc aatatccatc gggcgaccgt agtgtttctc
     1681 aatttgtacg gcctgttttg ccagttcctg cacttcttcg ttggtcagcg agaagatgtc
     1741 acgctgttcc tgcggtacgt cttcgatttt aacctgcttg ccgtgctcct gggtcggcgc
     1801 gtaaaccatg cggatttttt tcgaccccat ggtgcggcgc acgatagccg ggcgattcgc
     1861 cgccagtgtc ggtttatgca cgtaaaactc atccgggtta accgccccct gcacgaccat
     1921 ctcaccaagg ccccatgcgg aagtgataaa caccacctgg tcaaagccgg attcggtatc
     1981 aatggagaac atcacgccag aagatgcgag gtcggagcgc accatccgct gaacaccggc
     2041 ggagagcgcc acgccacggt gatcgtaacc ctggtgcaca cgataagaga tggcgcgacc
     2101 gttaaacaga gaagcaaata catgtttcac tgccacgaga acggcgtcaa acccctgaac
     2161 gttgaggaag gtttcctgct gaccggcaaa ggaagcgtcc ggcatatctt ctgcggtggc
     2221 ggaggagcgc accgcaaaag aggcgttttc gtcatcggcg gaaagctgag cataggcttc
     2281 gcggatggcg ttttccagct caggctggaa gggagtgtcg ataatccact ggcggatttg
     2341 cgcgcccgct ttcgcaagct gagtaacatc gtcaatatcc gttttatcca gcagttcata
     2401 aatgcgctgg tttacgccgc tttggtccag aaactggtta aacgcgtcgg cggttgtggc
     2461 gaaaccattc ggaacggaaa cgcccattcc ggaaaggtta gtaatcattt cacccaggga
     2521 ggcatttttg cccccaaccc tgtctacatc attcatgccg agttggttgt accaaagcac
     2581 cagcggtgac gagccattgt tggacatcga acaatccttt tgtgataaat gaacggtttg
     2641 agaaacacat ttctgcgcat ttatctttgc atatttaacc ggatgaaaaa aacggtgaat
     2701 cgttcaagca aatatatttt tttacttttt aagactgatc
//
LOCUS       DAJVUJ010000275         2685 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.278, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000275 DAJVUJ010000000
VERSION     DAJVUJ010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.278"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(52..236)
                     /locus_tag="OQQ48_004175"
                     /pseudo
     CDS             complement(52..236)
                     /locus_tag="OQQ48_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311258.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
     gene            complement(398..472)
                     /locus_tag="OQQ48_004176"
     tRNA            complement(398..472)
                     /locus_tag="OQQ48_004176"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(437..439),aa:Arg,seq:cct)
     gene            complement(548..1480)
                     /locus_tag="OQQ48_004177"
     CDS             complement(548..1480)
                     /locus_tag="OQQ48_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCS1954715.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETHDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            1774..2529
                     /gene="mlaA"
                     /locus_tag="OQQ48_004178"
     CDS             1774..2529
                     /gene="mlaA"
                     /locus_tag="OQQ48_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HCS1954716.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
BASE COUNT          654 a          637 c          656 g          738 t
ORIGIN      
        1 acccatcttt tacggggtga aggtagattt cgtatgaagg gtatctgttg tctcctgcaa
       61 agtcataagc ggatattttc ctacggtcag tatttttttc ttaccgtcaa tcttgtagcg
      121 aagttgccat accttttccc ggatacagga acataaaggt acaggccatt accatcgaga
      181 aggcggtatg gtttttcttt cggctttgct gcttcaatct gcttcacggt gagcatgggt
      241 aaaaatccgg tgggtaaaat tattttatcc actttttacc cgtcatggag tgcggctgtc
      301 aacgatctga cgcgaaccat gacgaaccgt gaatatacgg aaggcttgat attcagggga
      361 ttttgcggac tggtacggat gggagcgaac tgataaatgg tgtcccctgc aggaatcgaa
      421 cctgcaatta gcccttagga ggggctcgtt atatccattt aactaagagg acaatgcggc
      481 atgagtatac ccgctaatgg actgcggagt aagtacactg ccgctcgatt gcttaaaccc
      541 tcgccattta tgctgggttt ttatcatttt tcttaatgtt ttccgcacgt tctgcttttt
      601 ggcgtgcttc tgctttgcgc ttgttgctca tgtcgttacg gatctgtgca tgactcatta
      661 acgcgaagat aaaggtgccg ccgcagatat tccccgctaa agtaggtagt gcgaagggcc
      721 aaatgaaatc gctccagtgc agcgtgccgt taaacaccag atagaggatt tctacagaac
      781 cgacaacgat gtgggtggtg tcacccagtg caataagcca ggtcatcaat ataatcacca
      841 caatctttgc cgcacccgct gcaggaaaca tccaaaccat agtggcgatc agccagccgg
      901 aaatgatcgc gttggcaaac atctcgctgg gggtgttctt catcacatcc atgccgattt
      961 tgacaaatgc atcgtgagtt tcttcattga agataggcat atattcaaat gcccatgcag
     1021 caatacctgt cccgagaata ttacccagca gcacgacgcc ccataatcgc ataagtaagc
     1081 cgacgttgct cattgtcggt ttttgcatga cgggtagtac cgcagtcacg gtgttttcgg
     1141 taaataattg ctggcgggcc ataatgacga taataaaacc aaaggtataa ccgagattct
     1201 ccaacaagaa gctgcccggc acgccttcca gttcgacatg aaatatccct tttgccagta
     1261 acgaagcgcc catcgacaga cccgccgcaa tggctgacca cagtagtgcc attgcgtcgc
     1321 gttccagctc tttttcacca tcctggcgga tatgttcatg aattgccatc gcccgggagg
     1381 ggagtcgatc ttcatctatt tctatttttt tgccgcgctc tttttcttcg ctctcaactt
     1441 caatttcgtc gctgtgttga tcaattttgt cgttgtccat ggtctcttct cttgaattag
     1501 cacgtatagc taaagcgtag cggctttttt gctcgcaact ggcgggagtt actctgaaaa
     1561 tgtagaaaag gccgcgtttg cctttttctg tttctataga atcaagtagc ctacagggcg
     1621 gcgattacca ggctatgatc aaatcagcaa atcagggcgt ctggacatca gttgacgtgc
     1681 tgttacaatc gcccacacct aaacaggcgg atacggtatc gttccgtcat ggatggcaaa
     1741 ctgcataagc cataaaaaaa cagggagaca tttatgaagc ttcgcctgtc ggcgcttgct
     1801 ctgggaacta cgcttctggt ggggtgtgcg agttccggta cagatcagca agggcgttct
     1861 gacccgttag aagggttcaa ccgcaccatg tacaacttca acttcaatgt attagacccg
     1921 tatattgttc gaccggtcgc tgtcgcctgg cgtgattatg ttccgcaacc ggcgcgtaac
     1981 ggtttgagca actttaccgg caaccttgaa gaacctgcgg tgatggttaa ctacttcttg
     2041 cagggcgacc cttatcaggg gatggtccac tttacccgct ttttcctgaa caccattttg
     2101 gggatgggcg gctttattga tgttgcaggg atggcgaacc cgaaactgca acggactgaa
     2161 cctcaccgct tcggtagtac gcttggtcat tatggcgtgg gttatgggcc ttacgttcag
     2221 ttaccgttct acggtagctt cacgctgcgt gatgacggtg gtgatatggc ggatggtctt
     2281 tacccggttc tttcctggct gacctggccg atgtctgtgg gtaaatggac gcttgaaggg
     2341 atcgaaaccc gcgctcagct gctggattcc gatggtctgc tgcgtcagtc gtccgatcct
     2401 tatattatgg tgcgcgaagc gtacttccag cgtcatgatt tcatcgctaa tggcggcgaa
     2461 ctcaaaccgc aggaaaatcc gaacgcacaa gcgattcagg atgatttaaa agatattgat
     2521 tctgaataag aaacaaataa aaaaggtgag tcgcaaaact cacctttttt gttgttatcc
     2581 ctcaattacc acaagtaaaa ggagataaac cgacaatgac ccggacgtaa tgatgtaaaa
     2641 atactgatgt tcgtcactga ctatttccga tattcaggcg ttgaa
//
LOCUS       DAJVUJ010000276         2668 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.279, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000276 DAJVUJ010000000
VERSION     DAJVUJ010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2668
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.279"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            231..770
                     /locus_tag="OQQ48_004179"
     CDS             231..770
                     /locus_tag="OQQ48_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HCS1954717.1"
                     /translation="MSHGGWKEISHPQTLFTRQKGDGWTVVPKNLRIDFYTKDNDFTK
                     GLSVLSEVNKRHTEAQKGLTPSLNISKDDLKVLANARNIPQSKLEEEMMSQAIYRKEY
                     ATSNEKIKNYALYYHEQAQNIIQRHQDGLGNKNIDIAFITDKNHKKHLSDIFKVINES
                     GVKYDVIHFGACRVNREEK"
     gene            complement(996..1319)
                     /locus_tag="OQQ48_004180"
     CDS             complement(996..1319)
                     /locus_tag="OQQ48_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nikA protein"
                     /protein_id="HCS1954718.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLR
                     AAALGKNVNSLTDDRVLKEVMRLGALQKKLFIDGKRIGDREYAEVLIAITEYHRALLS
                     RLMAD"
     gene            1592..1786
                     /locus_tag="OQQ48_004181"
     CDS             1592..1786
                     /locus_tag="OQQ48_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCS1954719.1"
                     /translation="MNKQQQAVLNMAGFIKSQSLTLLEKLDALDADEQATMCEKLHEL
                     AEELQNSIQTRFEVENSTER"
BASE COUNT          863 a          656 c          564 g          585 t
ORIGIN      
        1 aaaaaaaaac aataaaaaac accgcaaaaa tggattgtta acaaatgaaa atactcaatt
       61 cgaatctcat aacaaacata tcatcaacaa taagaaatca ccttctaaca tcatccaaaa
      121 atataaaaac acacccatca ccaaacatgg acgtaacaag tcatcgtcgt ttattccaga
      181 aagacggaat aggattgagc caaacaggaa aagcaacaca acttgtcatt atgtcgcatg
      241 gtggatggaa agagatctcc catccacaaa cactattcac gcgtcaaaaa ggcgatggat
      301 ggactgtagt ccccaaaaac ctaagaattg atttttatac aaaagacaac gacttcacaa
      361 agggactaag cgttctttca gaggtaaaca aacgccatac tgaagctcaa aaagggttaa
      421 caccatcact aaacataagc aaagatgacc taaaagttct agcgaacgcg cgcaatatac
      481 ctcaatcaaa attagaagaa gaaatgatgt cacaagccat atatagaaaa gaatacgcaa
      541 caagtaatga gaagataaaa aactatgctc tatattacca tgaacaagcc caaaatatta
      601 tacaaagaca tcaagatggc ttaggaaaca aaaatattga tatagcattt ataactgata
      661 aaaatcataa gaagcacctt tctgacatat ttaaagtaat aaatgaatca ggagtaaaat
      721 atgatgtaat tcactttggc gcttgccgag taaatagaga ggagaaataa tcatcattta
      781 ccataatccc ttaattgtac gcaccactga aacgcgctga acgcgattct gacgcagaca
      841 ccacaaaaat agatgtttta ccccggaaaa acaggaagga tgcgtcagga tcgttttcag
      901 ctcgctgcat agctatgcat gaaagcgaat ggagatcact ttgggagctt accgtgttca
      961 taccgtctgt tttcaacagt tttctccccg ggaagctaat ctgccataag cctggataac
     1021 agggcacggt gatactccgt aatagcgatc agcacctccg catactccct gtccccgata
     1081 cgcttgccgt cgataaagag ttttttctgt aacgccccca gtctcataac ttctttcagt
     1141 acccgatcat cagtcagtga gttaacgttc ttaccgagag ctgccgcccg taaaaaaccg
     1201 gaaacggtct tgccgcattc agcagccctt tttctgattt cctggtcttc ttctgccgtc
     1261 agacgaacag ggcgactgat agcccgcctg cgcgtattac ttccacttcg ttttgtcata
     1321 aaacatggct ccgtatctga catgggtgtc ggggcaaagc cctgaccagg gcaattgtaa
     1381 tagcgtgcat gtatgcgcgg tataacaatt gcacatcctg tcctgtcagc aagctcgaat
     1441 taccgacaaa ggcacggcag ttttttggca gttacagtgt tgtctgacag aaatgcccgt
     1501 caaaaagcca ggcggcagca aaacagtcga aaagtggtac gcgatataat cacataacgc
     1561 agatgacaga cagaacggag aaaacgaaaa gatgaacaag cagcagcaag ccgtactcaa
     1621 catggcggga ttcataaaaa gccagagcct gacgctgctc gaaaaactgg acgcactcga
     1681 tgctgacgag caggccacca tgtgtgagaa actacacgaa ctcgcagaag aactccagaa
     1741 cagcatacag acacgctttg aagtagaaaa tagcacagag agataacgct caggacagga
     1801 tgtcgaacgg cacaacagag cgtaaggaga aaatgtcaca taaggcgctc ttccgcttcc
     1861 tcgctcactg actcgctacg ctcggtcgtt cgactgcggc gagcggtatc agctcactca
     1921 aaggcggtaa tgcggttatc cacagaatca ggggataacg ccggaaagaa catgtgagca
     1981 aaaagccgaa ggcagggaca aaggccgcgc aggtggcgtt tttccatagg ctccgccccc
     2041 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     2101 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     2161 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     2221 cacgctgttg gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     2281 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     2341 cggtaagaca cgaattatcg ccactggcag cagccactga taaccgaatt agaggagtaa
     2401 gacttgaagt catgcgcctg ttaaggctaa actgaaagaa caagttttgg tgactgcgct
     2461 cctccaagcc agttacctcg gctcaaagag ttggtatctc tgagaacctt cgaaaaaccg
     2521 ccttgcaggg cggttttttc gtttacaggc agtagattac gcgcagaaaa aaaggatctc
     2581 aagaagaacc tttaattttt tttcacactt ggtatcctac tttaacccat aaaataaaca
     2641 cttacaccga cacgccctta aacaaaag
//
LOCUS       DAJVUJ010000277         2653 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.280, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000277 DAJVUJ010000000
VERSION     DAJVUJ010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.280"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            29..865
                     /locus_tag="OQQ48_004182"
     CDS             29..865
                     /locus_tag="OQQ48_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1954720.1"
                     /translation="MQKNGDTLSGGLTFENDSILAWIRNTDWAKIGFKNDADGDTDSY
                     MWFETGDNGNEYFKWRSRQSTTTKDLMTLKWDALNILVNAVINGSLGVGSTNALGGSS
                     IVLGDNDTGFKQNGDGILDVYANSQRVFRFQNGVAIAFKNIQAGDGKKFMLSSSNNST
                     KNATFNLWGASTRPVVAELGNEAGWHFYSQRNTDNSVIFSVNGQIQPSNWGNFDSRYV
                     KDVRLGTRVVQLMARGGRYEKAGHAITGLRIIGEVDGDDEAIFRPIQKYINGTWYNVA
                     QV"
     gene            834..1397
                     /locus_tag="OQQ48_004183"
     CDS             834..1397
                     /locus_tag="OQQ48_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000930007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCS1954721.1"
                     /translation="MAHGITSHRCKLCSILKNIKSGNPKTKEQYQLTKNFDVIWLWSE
                     DGKNWYEEVNNFQDDTIKIVYDENNIIVAITKDAPTLNPEGFSVVEIPDITANRRADD
                     SGKWMFKDGTVVKRIYTADEQQQQAESQKVALLSEAESVIQPLERAVRLNMAMDEERT
                     RLESWERYSVLVSRVDTANPEWPQKPE"
     gene            complement(1426..1959)
                     /locus_tag="OQQ48_004184"
     CDS             complement(1426..1959)
                     /locus_tag="OQQ48_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCS1954722.1"
                     /translation="MMHLKNITAGNPKTKEQYQLTKQFNIKWLYSDDGKNWYEEQKNF
                     QPDTLKMVYYHNGVIICIEKDVSAINPEGASVVELPDITANRRADISGKWLFKDGVVI
                     KRTYTEEEQRQQAENEKQSLLQLVRDKTQLWDSQLRLGIISDENKQKLTEWMLYAQKV
                     ESTDTSSLPVTFPEQPE"
     gene            complement(1962..2519)
                     /locus_tag="OQQ48_004185"
     CDS             complement(1962..2519)
                     /locus_tag="OQQ48_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1954723.1"
                     /translation="MAGVGFDGSSDISISAKNVNAFALRQTGNTVNGDTSVGWNWDSG
                     AYNALIGGASALILHFNINAGSCPAVQFRVNYKNGGISYRSARDGYGVELGWSDFYTT
                     TRKPSAGDVGAYTRAECNSRFITGIRLGGLSSVQTWNGPGWSDRSGYVVTGSVNGNRD
                     ELIDTTQARPIQYCINGTWYNAGSI"
BASE COUNT          757 a          566 c          591 g          739 t
ORIGIN      
        1 ggaaacggta aacagggctg gtaacgccgt gcaaaaaaat ggcgatacct tgtccggtgg
       61 gcttactttt gaaaatgact caatccttgc ctggattcga aatactgact gggcgaagat
      121 tggatttaaa aatgatgccg atggtgacac tgattcatac atgtggtttg aaacaggcga
      181 caacggcaat gaatatttca aatggagaag ccgccagagc accacaacaa aagacctgat
      241 gacgctgaaa tgggatgcac taaatattct tgttaatgct gtcattaatg gcagtcttgg
      301 agttggttcg acgaatgcgt taggtggtag ctctattgtt cttggtgata atgacaccgg
      361 atttaaacag aatggagacg gtattctgga tgtttatgct aatagtcagc gtgtattccg
      421 ctttcagaat ggagtggcta ttgcttttaa aaatattcag gccggggatg gtaaaaaatt
      481 catgctatcc agctccaaca actccacaaa gaatgcaacg tttaatttat ggggtgcttc
      541 aacccgtcca gtagttgcag agttaggcaa tgaggcagga tggcatttct atagccagcg
      601 aaatacagat aactcggtaa tattttctgt taacggtcag atacagccca gcaactgggg
      661 gaattttgat tcacgctatg taaaagatgt tcgcctgggt acgcgtgttg ttcaattgat
      721 ggcgcgtggt ggtcgttatg aaaaagccgg acacgcaatt accggattaa gaatcattgg
      781 tgaagtagat ggcgatgatg aagccatctt cagaccaata caaaaataca tcaatggcac
      841 atggtataac gtcgcacagg tgtaaattat gcagcatatt aaaaaatatt aagtccggaa
      901 atccaaaaac aaaagaacaa tatcagctaa caaagaattt tgatgttatc tggttatggt
      961 ccgaagacgg taaaaactgg tatgaggaag taaataactt tcaggacgac accataaaga
     1021 ttgtatacga cgaaaataat attattgttg ccataaccaa agatgcccca acgcttaatc
     1081 ccgaaggctt tagtgtcgtt gagattccag atataacagc caatcgtcgt gccgatgatt
     1141 cagggaagtg gatgtttaag gacggaactg tggttaagcg gatttataca gcagacgaac
     1201 agcaacaaca ggccgaatca caaaaggtcg cgttgctttc tgaagctgaa agcgttattc
     1261 agccactgga acgcgctgtc aggctgaata tggcgatgga tgaggaacgc acacgactgg
     1321 agtcatggga acgctacagc gttctggtca gccgtgtgga tacagcaaat cccgaatggc
     1381 cacaaaagcc tgagtaaaaa ttaaggccca atatcgggcc ttctctcatt ctggttgttc
     1441 gggaaacgtt actggcaggc tggaggtgtc tgtagattcg actttctgcg catagagcat
     1501 ccactcggtt aatttttgtt tattctcgtc ggaaatgatg cccagccgta gctgtgagtc
     1561 ccatagctgg gttttatccc tgacaagttg caacaggctt tgcttttcat tttccgcttg
     1621 ttgcctctgc tcttcctcgg tataagttcg ctttatcact acgccatctt tgaacaacca
     1681 tttccccgaa atatcagccc ggcgatttgc tgtaatatca ggtaattcaa cgacgcttgc
     1741 gccttccgga ttaattgctg aaacatcctt ttcaatacaa ataataacgc cgttatggta
     1801 atagaccatt ttcaaagtgt ctggctggaa attcttttgt tcctcatacc agttttttcc
     1861 atcatctgaa taaagccatt tgatgttaaa ttgctttgtt agctggtatt gctcttttgt
     1921 tttagggttg ccagcagtaa tgttttttaa gtgcatcatc gttaaatact ccccgcgtta
     1981 taccacgtcc cattaatgca atactgaatt ggccttgcct gtgttgtatc aattaattca
     2041 tcacggtttc cgttaactga acccgtaacg acataacctg acctgtcaga ccagccggga
     2101 ccattccatg tctgaacaga tgacagaccg ccaaggcgaa tacctgtaat aaaccttgag
     2161 ttacattctg cccgcgtata tgcaccaaca tctcccgctg aaggttttcg tgtcgtggta
     2221 tagaaatctg accaacctaa ttcaacccca taaccatcac gagccgacct gtaggaaatg
     2281 ccaccatttt tataattcac acggaattgt acggcaggac agctaccagc atttatatta
     2341 aagtgaagaa ttaatgcaga tgcaccacca atcagggcgt tatatgcacc actatcccaa
     2401 ttccatccaa cggatgtatc accattaaca gtattacctg tttgtcggag tgcaaatgca
     2461 ttgacatttt tggcactaat tgagatatcg ctagaaccat caaaaccaac tccagcaatt
     2521 tttcgtgctg ttttcagctt tgttgcagta gcggcattgc cggacagttc cccagaaagg
     2581 ccaccgctga atgtttgtcg attggtccag gtattcgctg tactgagtaa cggtattttc
     2641 tccccgcttg tgc
//
LOCUS       DAJVUJ010000278         2614 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.281, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000278 DAJVUJ010000000
VERSION     DAJVUJ010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.281"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(924..1595)
                     /gene="queE"
                     /locus_tag="OQQ48_004186"
     CDS             complement(924..1595)
                     /gene="queE"
                     /locus_tag="OQQ48_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HCS1954724.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYDVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
     gene            1734..1874
                     /locus_tag="OQQ48_004187"
     CDS             1734..1874
                     /locus_tag="OQQ48_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1954725.1"
                     /translation="MSEENKENGFNHVKTFTKIIFIFSVLVFNDNESKITDAAVNLFI
                     QI"
     gene            1888..2614
                     /locus_tag="OQQ48_004188"
                     /pseudo
     CDS             1888..2614
                     /locus_tag="OQQ48_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
BASE COUNT          815 a          534 c          465 g          800 t
ORIGIN      
        1 tttaactagt tcggtttatc cctgttttta caaggaatat taactacact cgatgtcttt
       61 agaataaaag acaatacgaa tcgcacttta tcaattttca cttaaagtat gaactggata
      121 tctttattca taagaactat tcatcaacag cgtatagagg cggttatgta aaaccactca
      181 ttagcctcaa aacaaagaca aacttccaga tttattactt aaccaatgaa taacaagctt
      241 aaaaaatata ctgttattct aaagaaaaaa ttaacaggca ttaattcaat attaaaaaaa
      301 taatagatta aaatttctta acgatttaag aatcatacaa ataacacttt gattaaattt
      361 aattttttgt atcgataatt gtgaacctct ctggcatgga gaactatttt gaacatgagg
      421 tgttacgtgg atatgttgct tattacaagt actgctaata taaaaacttg agaaagagat
      481 aacgggttat atggtggttt atccccgctg gcgcgggaaa ctcccagctc ttgcccgcct
      541 caaagtgttg taactcggtt tatccccgct ggcgcgggga actctgtaaa cataacctat
      601 tattaattaa tgatttttta agccagtaac aatctaccaa ctttacagta tcacacaaac
      661 aacacatcca ttatgttaaa gagtacttaa ttcattgatt aaaaaggtaa atatttaaaa
      721 taactctata caaattaaaa tctaccaaac tttaccgcaa taattttcac tccagcgaaa
      781 aattaatgcc acagaatttg tagataatgc taatgattag ccctggatgg gtaaaatgga
      841 ggagttttca gaggcgacaa acaatataaa tgagtagaag tctttacagg ttccacccat
      901 ttataacgct tataaatgtt taatcaggca atatttagat atttatgtgt ttgcatcgac
      961 aaacgccaat tacgcgcaat gcaggtttca atgcacaaac gtgtggcatc atccttttgg
     1021 ctaatcggct gcagtgcaat gactcgcggt ttatcatcgg tcagcgttgc caatagttca
     1081 tcgagtgctt caatatcgcg tacgcgcccc actggatgct tgatttcgtt ggctcgctcc
     1141 agtgcctgtg acaacacgtc atagccgccg cgcatgttca gctttggcga tacggtaacc
     1201 caggtattcg gtgtgcagcg tacttcatga gttccactgg tttcgatctg gcagctaaaa
     1261 ccgttctttt cgagcaggtc agtcagtggc agcaaatcat gaatgcaagg ctcaccaccc
     1321 gtaatcacca catgccgcgc ggtatatccc tgccgaccaa tgacagccag caaatcttca
     1381 ctgctcgccg ccccccactt atcactctct ttggtcttcg ccagaatgct gaaaagggag
     1441 acttcccgat cctcaagctt ttcccaggtg tgtttggtgt cgcaccaggc acagccaacc
     1501 gggcatccct gtaaacgaat aaaaatggcg gggacgccgg taaagtaacc ctcaccttgc
     1561 agggtctgga acatctcgtt aatcgggtac tgcatagcat tctctgtgaa gtggataatt
     1621 gttaattatt gcagatcctg ccgcaacaat catgccttat taacattctg ttacatgcag
     1681 atttaaagaa aaatgcgaaa catatcgtta ataattaaag ggagtaacgt attatgtcag
     1741 aagaaaataa agaaaatgga tttaatcatg tcaaaacatt caccaaaatt atatttattt
     1801 tttctgtatt agtttttaat gataacgagt ctaaaattac cgatgccgcc gtcaatttat
     1861 ttatccagat ttaatgagaa atttataatg cgctatttca tactaatgtt cactttcgta
     1921 tgttcctttg ttgcagccca accaactatt gtcccacaat tacagtaaca agttacggat
     1981 cttacgagta gcttaaactc acaagaaaag aaagaactga cgcacaagtt agaatctatt
     2041 ttcaataata cccaagtgca aattgctgta ttaattgttc ccacaaccaa agacgagaca
     2101 attgaacaat atgctacaag agtttttgac aattggcgtt taggagatgc caaacgtaat
     2161 gatgggatac tgatcattgt tgcctggtca gatcgtactg tccgcatcca ggtaggttat
     2221 gggctggaag aaaaggtaac cgatgctctg gctggagata tcatccgtag caacatgata
     2281 cccgcattta aacaacaaaa atttgctcag ggattagagc tagctataaa cgctttgaat
     2341 aaccaactca cttcacaaca ccaatatccg actaatcctt cagaaagtga atcagcgtct
     2401 tccagtgatc attattactt tgctattttt tgggtatttg cagtgatgtt cttccctttc
     2461 tggttttttc atcaaggcag taatttttgt cgcgcatgta aaagtggagt ctgtatttca
     2521 gcaatctatc ttttagattt attcctgttc tccgataaaa ttttttccat tgctgtattt
     2581 tccttctttt ttactttcac catatttatg gtct
//
LOCUS       DAJVUJ010000279         2574 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.282, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000279 DAJVUJ010000000
VERSION     DAJVUJ010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.282"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..2265
                     /locus_tag="OQQ48_004189"
     CDS             <1..2265
                     /locus_tag="OQQ48_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCS1954726.1"
                     /translation="KKVSQKKITFTANAEQLLQPQFTVEQLRELGIKVDEIPALAEKD
                     DDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQIALYRDARGYVSPSRWDDGIPTLFTN
                     YSFTGSDNRYRQGNRSQRQYLNMQNGANFGPWRLRNYSTWTRNDQASSWNTISSYLQR
                     DIKALKSQLLLGESATSGSIFSSYTFTGVQLASDDNMLPNSQRGFAPTVRGIANSSAI
                     VTIRQNGYVIYQSNVPAGAFEINDLYPSSNSGDLEVTIEESDGTQRRFIQPYSSLPMM
                     QRPRHLKYSATAGRYRADANSDSKEPEFAEATAIYGLNNTFTLYGGLLGSEDYYALGI
                     GIGGTLGALGALSMDINRADTQFDNQHSFHGYQWRTQYIKDIPETNTNIAVSYYRYTN
                     DGYFSFDEANTRNWDYNSRQKSEIQFNISQTIFDGVSLYASGSQQDYWGNNEKNRNIS
                     VGVSGQQWGIGYSLNYQYSRYTDENNDRALSLNLSIPLERWLPRSRVSYQMTSQKDRP
                     TQHEMRLDGSLLDDGRLSYSLEQSLDDDNNHNSSLNASYRSPYGTFSAGYSYGNDSSQ
                     YNYGVTGGVVIHPHGVTLSQYLGNAFALIDANGASGVRIQNYPGIATDPFGYAVIPYL
                     TTYQENRLSVDTTQLPDNVDLEQTTQFVVPNRGAMVAARFNANIGYRVLVTVSDRNGK
                     PLPFGALASNDDTGQQSIVDEGGILYLSGISSKSQSWTVRWGNQADQQCQFAFNTPDS
                     EPTTSVLQGTAQCH"
     gene            2276..>2574
                     /locus_tag="OQQ48_004190"
     CDS             2276..>2574
                     /locus_tag="OQQ48_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1954727.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKL"
BASE COUNT          715 a          623 c          603 g          633 t
ORIGIN      
        1 aagaaggttt cacagaaaaa aattacattt accgccaatg cagagcaact tctgcagcca
       61 cagtttacgg tagaacaact acgtgagctg ggtattaagg tggatgaaat cccggcgctg
      121 gctgaaaaag atgacgatag cgtgatcaac tcgcttgaac aaatcattcc cggtacagct
      181 gctgaatttg atttcaatca tcagcgactt aatttgagca ttccccaaat tgcactgtac
      241 cgtgatgcaa gaggttacgt ctccccttct cgttgggacg atggtatacc aacgctgttt
      301 accaactact cgtttacagg ttctgataac cgttaccgcc agggcaatcg tagccaacga
      361 cagtacctga atatgcaaaa tggtgctaat tttggcccct ggcgattacg taactattct
      421 acgtggacac gcaacgatca ggcgtcaagc tggaacacta tcagtagtta tttacaacgt
      481 gatatcaagg cgttgaagtc tcagttgctt ctgggagaaa gcgccaccag cggcagtatt
      541 ttttccagct acacctttac tggcgtgcaa ctcgcttccg acgataatat gttgccaaac
      601 agccagcgcg gatttgcccc aacggtacgc ggtatcgcaa acagtagtgc aatcgtgact
      661 atcaggcaaa atggttatgt gatctatcaa agcaacgtgc cagcgggtgc ctttgaaatt
      721 aacgatctct acccctcttc caacagcggc gatttagaag tcacgattga agaaagtgac
      781 ggtacacaac gtcgctttat ccagccttat tcttcattac ccatgatgca gcgacctagg
      841 catctaaaat atagcgcgac cgctggacgc tatcgcgctg atgcaaacag tgatagcaag
      901 gaacccgaat ttgctgaagc cacggcaata tatggtttga ataatacttt tacgctgtat
      961 ggcggcctgc tcggttctga agattattat gcgctgggga tcggtatcgg cggcacactt
     1021 ggcgcactgg gcgcgttgtc gatggatatc aacagagctg acacccaatt cgataaccag
     1081 cactcttttc atggctatca atggcgtacg cagtacatca aagatatccc ggaaaccaac
     1141 accaatatcg ctgttagcta ctatcgctat accaacgatg gctattttag ttttgatgaa
     1201 gccaataccc gcaattggga ctataacagt cgccaaaaaa gtgaaattca attcaacatc
     1261 agccagacaa tatttgatgg ggtaagtctg tatgcctccg gttcgcagca agactattgg
     1321 ggcaataacg agaaaaacag gaatatctct gttggggttt ccggccagca atggggaatt
     1381 ggttacagcc tgaattatca atacagccgc tacactgatg aaaataatga ccgtgcactc
     1441 tctttgaatc tcagtattcc gttagaacgc tggttaccgc gtagccgggt ttcctatcag
     1501 atgaccagcc agaaagatcg cccaacccaa catgaaatgc gtcttgatgg ctcactgctg
     1561 gatgatggtc gcctgagcta tagcctggaa caaagtctgg atgacgataa caaccataac
     1621 agtagcctga acgccagtta ccgttcacct tatggaacct tcagtgccgg atacagttac
     1681 ggtaatgaca gcagccaata caattacggc gttaccggcg gcgtggttat ccatcctcat
     1741 ggcgtgacgc tctcgcaata tctgggcaac gcttttgcgc ttatcgatgc taacggggct
     1801 tctggcgtga ggatacaaaa ctatccgggg attgctactg atccctttgg ctatgcagtg
     1861 attccttatc tcacgactta tcaggaaaac cgtctctcgg tagatactac gcagctgccc
     1921 gataacgtcg atcttgaaca aacaacacag tttgtggtgc ccaacagagg tgcaatggta
     1981 gcggcgcgtt tcaacgccaa tatcggttat cgcgtacttg ttacagtcag cgatcgcaac
     2041 ggtaaaccgt tgccctttgg cgctcttgcc agcaacgatg atacggggca acaaagtatc
     2101 gtcgatgagg gcggcatact atatctctct gggatatcga gtaaatcaca aagctggact
     2161 gtacgctggg gaaatcaggc agatcaacaa tgtcagtttg cttttaatac accggattca
     2221 gaaccaacaa cctctgtatt acaaggtaca gcgcagtgcc attaaggata aaaaaatgaa
     2281 aagagcgcct cttataacag gacttttgtt gatatccaca tcctgcgctt atgcctcctc
     2341 aggagggtgt ggagccgaca gcactagcgg tgcgacaaat tacagcagtg tggttgatga
     2401 tgttacggtg aaccagacag ataacgtgac aggacgggag tttacctctg caacgctaag
     2461 tagcactaac tggcaatacg cctgttcctg ctctgcgggt aaggcagtta aacttgtcta
     2521 tatggtcagc cccgtactta ccaccactgg acatcagaca ggatattaca aact
//
LOCUS       DAJVUJ010000280         2471 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.283, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000280 DAJVUJ010000000
VERSION     DAJVUJ010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.283"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            128..1264
                     /gene="potA"
                     /locus_tag="OQQ48_004191"
     CDS             128..1264
                     /gene="potA"
                     /locus_tag="OQQ48_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HCS1954728.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMREGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            1248..2111
                     /gene="potB"
                     /locus_tag="OQQ48_004192"
     CDS             1248..2111
                     /gene="potB"
                     /locus_tag="OQQ48_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HCS1954729.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVSELDD"
     gene            complement(2343..2414)
                     /locus_tag="OQQ48_004193"
                     /pseudo
     CDS             complement(2343..2414)
                     /locus_tag="OQQ48_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387760.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
BASE COUNT          594 a          607 c          621 g          649 t
ORIGIN      
        1 ctaagccagt aattcacaac aaagattggc gattcaagcg gtttgccgta gaattaccgc
       61 cctcattaag agtcaccaag gtggttaacc acaaaccccg catcggtaag ccatccgttg
      121 cgtttacatg ggacagagta aaaaattgaa taaacaaccg agttcgcttt caccgctggt
      181 gcaattggcg ggaattcgca aatgctttga tggtaaagag gtcattcccc agctggatct
      241 gactatcaac aatggcgagt tcctcacgct gcttggccct tctggctgcg gtaaaacaac
      301 cgttcttcgc ctgattgcag gtctggaaac tgttgattcc ggacgcatca tgctggataa
      361 cgaggacatc acccacgttc cggcggaaaa ccgctatgtg aacactgttt tccaaagcta
      421 cgcacttttc ccccacatga ccgtgttcga aaatgtggcc tttgggttgc gcatgcaaaa
      481 aacccccgct gctgaaatta cgccccgcgt gatggaagcc ctgcggatgg tgcaactgga
      541 aaccttcgct caacgtaaac cgcatcagct ctctggtggt caacagcaac gcgtcgccat
      601 tgctcgcgcg gtggttaaca agcctcgtct gttgttgctg gatgagtcgc tctcagcgct
      661 ggattacaaa ctgcgtaagc aaatgcagaa cgagctgaaa gcgttacagc gtaagcttgg
      721 cattacattc gtcttcgtga ctcacgacca ggaagaagca ctcaccatgt cagacaggat
      781 tgtggtgatg cgcgagggtc gcattgagca agacggcacg ccgcgtgaaa tctacgaaga
      841 gccgaaaaac ctgtttgttg ccggcttcat tggcgaaatt aatatgttta acgccacggt
      901 gatcgagcga ctcgacgagc agcgcgtacg cgccaacgtt gaaggccgcg aatgtaatat
      961 ctacgttaac ttcgccgttg aaccggggca aaaactgcat gttctgctgc gcccggaaga
     1021 cttacgtgtt gaagagatta acgacgacaa ccacgctgaa gggctgattg gttacgttcg
     1081 tgagcgtaac tacaaaggca tgacgctgga gtcggttgtt gaactggaaa atggcaagat
     1141 ggtgatggtc agcgaattct tcaatgaaga cgatcctgac tttgaccact ctctcgacca
     1201 aaaaatggcc attaattggg tagaaagctg ggaggtcgta ctggctgatg aagaacacaa
     1261 gtaagttcca gaatgtagtg attgtcacta ttgtcggttg gcttgtgttg tttgtctttc
     1321 tgcccaacct gatgatcatt ggcaccagct ttttgacccg cgacgacgcc agtttcgtca
     1381 aaatggtctt tacgctggat aactacacgc gtctgctcga tccgctctat tttgaagtgc
     1441 tattgcactc gctgaatatg gcgctgatcg ccaccctcgc ctgcctggtg ctgggctatc
     1501 catttgcctg gtttctggcg aagctgccac acaaggtgcg tccgctgctg ctgtttctgc
     1561 tgattgttcc gttctggacc aactcattga ttcgtatcta cgggctgaaa attttcctca
     1621 gcaccaaagg ctatctcaac gagtttttgc tctggctggg cgttatcgac acaccaatcc
     1681 gtatcatgtt cacgcccagt gcggtgatta tcggtctggt ttacattctg ctgccgttta
     1741 tggtgatgcc gctgtactcc agtatcgaaa aactggataa gccgttgctt gaggcggcgc
     1801 gcgatctcgg tgccagcaag ttacagactt ttatccgcat cattattcca ctgacgatgc
     1861 cggggattat tgccggatgt ctgctggtga tgctgccagc gatgggcctg ttctatgtgt
     1921 ccgacctgat gggcggtgcg aaaaacctgc tgatcggtaa cgtcatcaag gtacagttcc
     1981 tcaatattcg tgactggccg tttggtgcag ccaccagcat tacgctgact atcgtaatgg
     2041 gcctgatgtt gctggtttac tggcgcgctt ctcgtttgct gaataagaag gtgagcgaat
     2101 tagatgatta atcatttata ttcaatgcat taagtttgtt tttgagtggt tttgccgttt
     2161 aaggtaacgg cgttatagat cctatcaaaa tcaatcaatt ccattctggt tttggcgaaa
     2221 gatttttcga gagcttcggt ctttaactaa aactgattgc gtataacccc aatacccctc
     2281 cccgactatc tgaattgtcg gggaggcact tcacgcacaa aatatcatcc ctgcgccgtt
     2341 gattactctg caggtgaagt cggccactca atatcaggtg cagttgatgt atcaacacgg
     2401 ttcagcaaca cctgaagtgg cacactgaat ttggccacct gaacagaggt gatatgctca
     2461 cctcagaaca a
//
LOCUS       DAJVUJ010000281         2411 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.284, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000281 DAJVUJ010000000
VERSION     DAJVUJ010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.284"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..447
                     /gene="yeeE"
                     /locus_tag="OQQ48_004194"
     CDS             <1..447
                     /gene="yeeE"
                     /locus_tag="OQQ48_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HCS1954730.1"
                     /translation="VLIGLIALLAWPLSEATGRMFGLGITSPTANILQFLVAGDVKYI
                     NWGVFLVLGIFVGSFIAAKASREFRVRAADAQTTLRSGLGGVLMGFGASIAGGCSIGN
                     GLVMTAMMTWQGWIGLVFMILGVWTASWLVYVRPQRKARLATAAAN"
     gene            461..688
                     /locus_tag="OQQ48_004195"
     CDS             461..688
                     /locus_tag="OQQ48_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HCS1954731.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(731..2155)
                     /gene="sbcB"
                     /locus_tag="OQQ48_004196"
     CDS             complement(731..2155)
                     /gene="sbcB"
                     /locus_tag="OQQ48_004196"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HCS1954732.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDSEFNVIGE
                     PEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMPPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDSD
                     TLRERLYTAKTDLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPTDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQMLV
                     QQYADDKEKVALLKALWQYAEEIV"
BASE COUNT          581 a          575 c          621 g          634 t
ORIGIN      
        1 gtactcatcg gtttgattgc gcttttagcc tggcccctga gtgaagcaac cggacgcatg
       61 tttgggttag gaatcacttc cccaacggcc aatattctgc aatttctggt cgcgggtgac
      121 gtgaaataca ttaactgggg cgttttcctg gtgttaggga tcttcgtggg gtcatttatt
      181 gcagccaaag cgagccgtga gttccgcgtt cgcgcagctg atgcacaaac aacattacgt
      241 agcgggttag gtggtgtact gatgggcttc ggtgccagta ttgcaggtgg ttgctctatc
      301 ggtaatggac tggttatgac tgcaatgatg acctggcagg gctggattgg ccttgtattt
      361 atgattctcg gagtctggac tgcatcctgg cttgtgtatg ttcgaccgca gcgtaaggcg
      421 cgactggcga ctgctgcggc aaattaagta aggtgtaaag atggcgatta aaaagctgga
      481 tgtggtgacg caggtctgtc cgtttccact catagaagca aaagcagcac tggcagaaat
      541 ggccagcggc gatgagttgg taattgagtt tgattgcacc caggcaactg aagcaatccc
      601 acagtgggcg gcagaagaag gacatgccat caccgattat cagcagattg gcgatgcagc
      661 gtggagcatc accgttcaaa aagcctgact caacattgtc ctccgccgta ccagcggcgg
      721 aggcttcaaa ttagacgatc tcttccgcgt actgccaaag tgcttttaac agcgccactt
      781 tttctttgtc atcggcatat tgttgtacca gcatctgcaa ttcatcagca taaccctgca
      841 aaaactctgg cgtgaatacc tggcggcggt gctccagcca gcgttgctgc tcggcataat
      901 ccagcgtccc cgggaagttg cgtgcccgat aattgaacaa cagcttttca atccgtttat
      961 caacaaaagt gatatccagt gccggtaaat tacgtggctc ggtttccagc acaattttca
     1021 ttgctgcacg atctgcgtca ctgaaaaagc cgttataaag ctgtgcatcc acgttatctg
     1081 taggcgtaaa cggttcggct tccgcgaata tcgccaccac tttttcacgc acttgcggat
     1141 tttcacgcag aattttcagg ttatcgaggc aatgctgacg attaattccc agtcggtcgg
     1201 catcttccgg gcgtagcgta ttcgcctggg ccagcaccgg acatttattg atgtgcacca
     1261 gcttaaccgg aacggcggcg ttatcgccaa gatcggtttt tgcggtatat aaacgctcgc
     1321 gcaatgtgtc gctatccagt tccagtaatg gcgaaatgtc tcctgccaaa tccaccataa
     1381 ttacagcatt gcgattttca ggatgccacg ccagcggtgc cacccagctg gtattgccgc
     1441 gccatgcccc aaacattccg gaaacgtgaa ccaggggggg catctgcgga acatcaatca
     1501 acgccatcag tttgtgttta ttacgatggg taaagagata atcaaacagg cgtggctgac
     1561 gcgtttttac cagtttcgcc atcgcaatag tggcgtacac atcagccatc gcatcgtggg
     1621 cgttgctatg ttcaatacca ttcgctttgg ttaaatgctc aaggcgaaag ctcggtagac
     1681 cgtcatcatt ttcaggccag tttattcctt ccgggcgcag ggcataacag gcacgcataa
     1741 catccaacaa atcccagcgc gagttatcat gctgccagct ccaggcgtaa ggatcgtaga
     1801 aattacgata aaaaacgttg cgtgtgactt cgtcgtcgaa acgcacattg ttgtagccca
     1861 gaatacaggt cttcggtacg gtaaaaagcg agtgaatacg ggcggcaaac gcggcttcgt
     1921 tttctccttt cgcccgtgct tcctgcgggg taataccggt aattaatact gctccaggct
     1981 ggggtaaata gtcatccgcg ggcttgcagt aaaagacttc gggttcgccg atgacattga
     2041 attcgctatc ggtgcgaatg gctgcgaact gtgcagggcg atctaacgcg gggtgcgtgc
     2101 caaaggtttc gtaatcgtga aagaggaagg taggttgttt atctgtgtct gtcattgtct
     2161 agcattgtcc actgtagtta attaactgtc tgttataact cgaaaaacaa ccattttttc
     2221 acctgagtat gtctacgaac gtcaaatata gttgtttact gtgcgctgtt gtccatgtga
     2281 tattgtgtac cagatgtgta ccaaaccatt atttctgagc gtaccaaata ttatttatgg
     2341 ctataagcga cacaaaactg cgtagtattt atggtaaacc atattcgggc ccacaagaag
     2401 tggctgatgc c
//
LOCUS       DAJVUJ010000282         2327 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.285, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000282 DAJVUJ010000000
VERSION     DAJVUJ010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.285"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(464..655)
                     /locus_tag="OQQ48_004197"
     CDS             complement(464..655)
                     /locus_tag="OQQ48_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCS1954733.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDH
                     ADELYRSCLARFGDDGENL"
     gene            720..1043
                     /locus_tag="OQQ48_004198"
     CDS             720..1043
                     /locus_tag="OQQ48_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HCS1954734.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVFLGEPVARSGRLPT
                     LAPPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQG
                     TRDDS"
     gene            1033..>2327
                     /locus_tag="OQQ48_004199"
     CDS             1033..>2327
                     /locus_tag="OQQ48_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HCS1954735.1"
                     /translation="MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELI
                     DATPFAKKYTSGVLSFAEKALPAGGREKVMASFERVLMPGLEKNQYSILWVEHQDKGR
                     LELNFVIPNMELQTGKRLQPYYDRADRSRIDAWQTLVNHHYGLHDPNAPENRRTLTLP
                     DNLPETKQALAESITRGIDALYHVGEIKDRQDVIQALTEAGLEVVRVTRSSISIADPN
                     GGKNIRLKGAFYEQSFTDGRGVREKAERESRIYRENAERRVQEARRICKQGCDIKRDE
                     NQRRYSPVHSFDRGITEKTPGRGERGDDAAQEGRVKAGREYGHDVAGDGYFPVYREWR
                     DALVSWQDDTGEPGRNQEAERESGEAEREDMGRALSRGEQRTFSGVARGDESRNELDD
                     GKRETECRKAGTGVTEHDGAGKTFAERIRATATGLYAAA"
BASE COUNT          567 a          544 c          741 g          475 t
ORIGIN      
        1 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccatcggc
       61 gcggccttct tcctgttcct ggtcttttgc tcacatgttc tttccggcgt tatcccctga
      121 ttctgtggat aacagcattt cgtttttgag tgagtagata ccgctcgccg cagtcgaacg
      181 accgagcgta gcgagtcagt gagcgaggaa gcggaaaagc gcctggacgt gcattttctc
      241 cttacgcatc agtgcggcat ttcacacccg gtatggcgca cttttcatac aatccgcact
      301 gatgccgcat ggtgaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg
      361 cgccccgaca cccgctaaaa cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
      421 ccgctcacag ataagctgtg accgtctccg ggagctgcat gcgtcagagg ttttcaccgt
      481 catccccgaa gcgagcgagg caactgcggt aaagctcatc ggcgtggtcg tgaagcgatt
      541 cacagatgtc tgcctgttca tccgcgtcca gttcgttgag cttctccagc agcgttaatg
      601 tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgttta gtcactgatg
      661 cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgcgagagga
      721 tgctcacgat acgggttact gatgatgaac atgctcgttt gctggaacgt tgtgagggta
      781 aacaactggc ggtatggatg cggcgggtct tcctggggga gccggttgcc cgttccggga
      841 ggctgccgac actggcaccg ccgttactgc gtcagctggc cgccatcggg aataacctga
      901 atcagacagc gaggaaggtg aacagcgggc agtggtcttc cggtgaccgg gttcaggtgg
      961 tggccgcact gatggccatc ggggatgagc tgcgccggct gcgtctggcc gtcagggaac
     1021 aggggacgcg ggatgatagt taagtttcat gccaggggaa aaggtggtgg cagtggtccg
     1081 gttgattacc tgctgggaag ggagcgtaac cgggaagggg caacggtgct tcagggtaat
     1141 ccggaagaag tccgggaact catcgatgcc acgccatttg cgaagaaata cacgtccggt
     1201 gttctctcgt tcgcggagaa ggcgctgcca gcgggaggac gtgaaaaagt gatggcgagt
     1261 tttgagcgtg tactgatgcc cggtctcgaa aaaaatcagt acagcatcct gtgggtggag
     1321 caccaggaca agggacggct tgagctgaat tttgtcattc cgaacatgga gctacagacc
     1381 ggaaaacgcc tccagccgta ctacgaccgg gcagacaggt cgagaattga tgcctggcag
     1441 acgctggtga atcaccatta cgggctgcat gacccgaacg ccccggagaa ccgccggacg
     1501 ctgactctcc ctgataacct gccggaaacg aaacaggcgc ttgctgagag cattacacgg
     1561 gggatagacg ctctttatca tgtcggggag ataaaagacc gtcaggatgt gattcaggcg
     1621 ctcacagagg cggggcttga agtggtcagg gtgacgcgaa gcagtatcag cattgcagac
     1681 ccgaacggcg ggaagaatat cagactgaaa ggagcattct atgagcaatc ttttacagac
     1741 gggcgcggag ttcgagaaaa agctgaaaga gagagccgaa tctacagaga aaatgctgaa
     1801 cgacgagttc aggaagctcg aagaatctgt aagcaaggct gtgacatcaa acgagacgaa
     1861 aatcaaagac gctatagccc tgttcacagc ttcgacagag gaatcactga aaaaacaccg
     1921 ggaaggggtg aaagaggcga tgatgcagca caggaaggac gtgttaaagc tggcagggaa
     1981 tacgggcatg atgttgctgg ggatggttat tttcctgttt accgtgagtg gcgggacgct
     2041 ctggtatctt ggcaggatga tacaggcgaa cctggaagaa atcaggaagc agaacgagag
     2101 tctggagaag ctgaacgcga agacatgggg cgtgcgttat cacgagggga gcaacggacg
     2161 ttttctggtg ttgcccgagg ggatgaaagc cgaaacgaac tggacgatgg aaaacgggaa
     2221 acagaatgcc gtaaggctgg tacaggagta acagagcatg acggagctgg aaagacattt
     2281 gctgaacgca ttagagcaac tgcaacagga ctatatgcag cggctga
//
LOCUS       DAJVUJ010000283         2281 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.286, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000283 DAJVUJ010000000
VERSION     DAJVUJ010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.286"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..184
                     /locus_tag="OQQ48_004200"
     CDS             <1..184
                     /locus_tag="OQQ48_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HCS1954736.1"
                     /translation="IYQPVYSPWVNHVERLWQALHDTITRNHQCRSMWQLLKKVRHFM
                     ETVSPFPGGKHGLAKV"
     gene            complement(204..455)
                     /locus_tag="OQQ48_004201"
     CDS             complement(204..455)
                     /locus_tag="OQQ48_004201"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1954737.1"
                     /translation="MPGTTTAMSINFIGITARTMNSNGSHGKPQIPVDYQKLLSIEDI
                     TFCRNRWGNIGENALRRVAVGKKLSFFGSDRGGENAAII"
     gene            378..1067
                     /locus_tag="OQQ48_004202"
                     /pseudo
     CDS             378..1067
                     /locus_tag="OQQ48_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(1084..1263)
                     /locus_tag="OQQ48_004203"
                     /pseudo
     CDS             complement(1084..1263)
                     /locus_tag="OQQ48_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1593..>2281
                     /gene="ipaH7.8"
                     /locus_tag="OQQ48_004204"
     CDS             1593..>2281
                     /gene="ipaH7.8"
                     /locus_tag="OQQ48_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH7.8"
                     /protein_id="HCS1954738.1"
                     /translation="MFSVNNTHSSVSCSPSINSNSTSNEHYLRILTEWEKNSSPGEER
                     GIAFNRLSQCFQNQEAVLNLSDLNLTSLPELPKHISALIVENNKLTSLPKLPAFLKEL
                     NADNNRLSVIPELPESLTTLSVRSNQLENLPVLPNHLTSLFVENNRLYNLPALPEKLK
                     FLHVYYNRLTTLPDLPDKLEILCAQRNNLVTFPQFSDRNNIRQKEYYFHFNQITTLPE
                     SFSQLDSSYRI"
BASE COUNT          676 a          513 c          429 g          663 t
ORIGIN      
        1 catttatcag ccggtttact cgccatgggt gaatcatgtt gaacggctat ggcaggcact
       61 tcacgacaca ataacgcgta atcatcagtg ccgctcaatg tggcaactgt tgaaaaaagt
      121 tcgccatttt atggaaaccg tcagcccatt ccccggaggc aaacatgggc tggcaaaagt
      181 gtagcggtat taagcgcagc tatttagatt attgccgcat tttctccacc acgatcagag
      241 ccgaaaaaag ataatttttt tccaactgcc acccggcgta atgcgttttc accaatgtta
      301 ccccacctat tgcggcaaaa ggtaatgtct tcaatcgata gaagtttctg gtaatctact
      361 ggtatttgag gttttccatg actaccgtta ctgttcattg tccgcgctgt aattccgatg
      421 aagtttatcg acatggccgt agttgttccc ggcatgagcg tttccgttgt cgctcatgta
      481 aacgtgtttt ccagctcact tactcttatg aagccagaaa actcggcgtt aaggagcaga
      541 tcgttgaaat ggcacataac ggcgcagggg gctgttatac cgccagaaca ctgaaaatcg
      601 gcatcaatac cgtcatacgg acattaaaaa gctctcgccc cggcggataa catctgcgcc
      661 agtcgctcac gctgatgttg cccttatctg tgaacttgat gagcagtggc gcttcgttgg
      721 cagcaaggcg caacaacact ggccctggta tgcgtacaac actaaaactg gggtgtgttg
      781 gcttacactt gcgggccacg gaatgatgag acctgtcggg aactgttagc cctgttgact
      841 ccgttctgta tcggcatggt gaccagcgat gactggggca gctacgccag agaagtgccg
      901 gaggagaaac acctgaccgg caaaatcttc acgcaacgta tgaacgtaac aacctgaccc
      961 tccgcccccg catcaagcgg ctggcacgta aaacaatctg cttctcgcgt tccgtggaaa
     1021 tccacgaaaa agtcatcggt tccttcattg aaaagcacat attctaatga ttggaagtac
     1081 tacccggcaa aactcattca gtgcattcca gtgattcagg atatagttga acgcctttac
     1141 catttcagca tggtttgaca aagttttcct ctggagcttt atccagtcat acaaggactt
     1201 cattcgcggc acacatttct cctgccttac cgcaagccgt ttttctgccg gcctccctcg
     1261 tattaaatgt gtatcgtttt tttacagtaa ttttaattta tcattctata ataggaataa
     1321 atgagcttgg ctaaaatatg atataaaaca aatatactta ctcggattac atttccttgc
     1381 tcactggaat gttgaattca gtgttattat tgactgcagt cacaataaaa aaaagaattc
     1441 catagaaaac ctcaacaaat aaattacaac taacttctgt tatgtgtaaa atggaaacta
     1501 ttaaaactta atatcgaaaa tggtaagtga aatttgtata aatatacaat tttaaatatt
     1561 tattctcaca aatataaggt tgccctagca ttatgttctc tgtaaataat acacactcat
     1621 cagtttcttg ctccccctct attaactcaa actcaaccag taatgaacat tatctgagaa
     1681 tcctgactga atgggaaaag aactcttctc ccggggaaga gcgaggcatt gcttttaaca
     1741 gactctccca gtgctttcag aatcaagaag cagtattaaa tttatcagac ctaaatttga
     1801 cgtctcttcc cgaattacca aagcatattt ctgctttgat tgtagaaaat aataaattaa
     1861 catcattgcc aaagctgcct gcatttctca aagaacttaa tgctgataat aacaggcttt
     1921 ctgtgatacc agaacttcct gagtcattaa caactttaag tgttcgttct aatcaactgg
     1981 aaaaccttcc tgttttgcca aaccatttaa catcattatt tgttgaaaat aacaggctat
     2041 ataatttacc ggctcttccc gaaaaattga aatttttaca tgtttattat aacaggctga
     2101 caacattacc cgacttaccg gataaactgg aaattctctg tgctcagcgc aataatctgg
     2161 ttacttttcc tcaattttct gatagaaaca atatcagaca aaaggaatat tattttcatt
     2221 ttaatcagat aaccactctt ccggagagtt tttcacaatt agattcaagt tacaggatta
     2281 a
//
LOCUS       DAJVUJ010000284         2278 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.287, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000284 DAJVUJ010000000
VERSION     DAJVUJ010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.287"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            368..2020
                     /locus_tag="OQQ48_004205"
     CDS             368..2020
                     /locus_tag="OQQ48_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCS1954739.1"
                     /translation="MIPLKKNITLIMFTLSLLTGNPAIAYETDKVYKITVLHTNDHHG
                     HFWRNNHGEYGLSSQKTLVDNIRQKVINNGGSVLLLSGGDINTGVPESDLQKAEPDIR
                     GMNLIGYDAMAVGNHEFDNPLNILRQQEKWATFPFLSANIYQKSTGRRLFSPWKIFTR
                     QNLKIAVIGLTTDDTAKTGNSEYFTDIEFRQPAAEARSVIDELNQQEKPDIIIAATHM
                     GHYDNGESGSNAPGDVEMARSLPTGSLAMIVGGHSQAPVCMASDNKKQWNYIPGTTCV
                     PDKQNGIWIVQAHEWGKYVGQADFEFCNGTMKLVNYQLHPVNLKMRITREDGKTEFSF
                     YTPEITEDPQMLSLLTPFQNKGKAQLDVKVGVVNGRLEGDRSKVRFVQTSMGHLILSA
                     LTERIDADFAVVSGGEIRDSIESGNITYKDILKVQPFGNTVVSIDLTGKEVADYLATV
                     AQMKPDSGAYPQFLNTSFVVKKGKIEMLKIKGKSVDLNKKYRMTTFSFNATGGDGYPR
                     IDNRPGYINTGFIDAEVLIEYIREHSPLDAASYEPKGEVSWQ"
     gene            complement(2007..2207)
                     /locus_tag="OQQ48_004206"
     CDS             complement(2007..2207)
                     /locus_tag="OQQ48_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1954740.1"
                     /translation="MTKKLAALQHQLSRKVKFSNNRQKQKHKIQQLHSHIANIRRDYF
                     HKVTATVSKKHVMIVNYVITAN"
BASE COUNT          706 a          421 c          534 g          617 t
ORIGIN      
        1 atcggaaacg atgatctggc agcctgagtt cacagataaa acattctcca ggaaacttgg
       61 ggcggttcac gtcagtatca gaccatatta attgctgaaa ctgataaaca tcaaacctta
      121 tagaagggtg aatgctgaaa gggggctatg gtgatcgacg tgatgacaac gacaggtaag
      181 accgtgacct gataaacctg aattgtgtcc tagacataaa gtcggtgaag ggaataaggc
      241 tcaggtcagc gaatcttata gtggcccgca gctctgactg caataaggcc ggatacagag
      301 cagcaactac tcgtcgatgt cagaacaacg gaagccttgc aaacggggat gcgttcatac
      361 aggtacaatg ataccgttaa aaaaaaacat tacgctaata atgttcaccc tgtcattact
      421 gacaggaaat cctgctatag cgtatgaaac agataaagta tataaaataa ccgttctgca
      481 tacaaatgac caccatggac acttttggcg taacaatcat ggtgagtacg ggttatcctc
      541 ccaaaaaact ctggtggaca atatccggca gaaagtaata aataatggcg gaagtgttct
      601 attattatct ggtggtgata ttaacaccgg tgtacctgaa tcagaccttc agaaggctga
      661 acccgatatt cgtggaatga atctaattgg ttatgatgca atggccgtcg gtaatcatga
      721 atttgataat cctctaaata tattacgtca gcaggaaaaa tgggccacat ttcctttcct
      781 ttctgctaac atatatcaaa aaagtacagg gaggcgttta ttcagtccat ggaaaatttt
      841 cacccgtcag aatttgaaaa ttgcagtcat tggcttgacg acagatgata ctgcgaaaac
      901 aggaaattct gaatatttta ctgatattga gttccgtcaa cctgctgctg aggcgaggag
      961 tgtcattgat gaacttaatc agcaggaaaa gccagatatt attattgcgg caacacatat
     1021 ggggcactat gacaatggcg agtcaggttc gaatgctcct ggtgatgttg aaatggctcg
     1081 cagtttgccg actggctcat tagcaatgat tgtgggagga cattcacaag ctcctgtctg
     1141 catggccagc gataataaaa aacagtggaa ttatattccg gggacaacct gtgtaccaga
     1201 taaacaaaat ggcatctgga ttgtacaagc gcatgagtgg ggaaaatatg tcggtcaggc
     1261 tgattttgaa ttctgtaacg gtacaatgaa actggttaat tatcagttac acccagttaa
     1321 tctcaaaatg agaattacca gagaagatgg aaaaacggaa ttttcttttt acacccccga
     1381 aattactgaa gatccacaga tgttatcctt actgacacca ttccagaata aaggcaaagc
     1441 acaactggat gtgaaggttg gggttgtgaa cggacgtctg gaaggggaca gaagtaaagt
     1501 tcgttttgtt cagaccagta tgggccatct gattctgagc gctctgacag aacgtattga
     1561 cgcagatttt gctgttgtga gtggtgggga aattcgagat tccatagaga gtggtaacat
     1621 cacatacaaa gatatactga aagttcagcc atttggtaat acggtagtaa gtattgattt
     1681 gacggggaaa gaagtagctg actacctagc tacagtggca cagatgaaac cggattccgg
     1741 agcctaccca caatttttga ataccagctt tgtagtcaaa aaaggaaaaa tcgaaatgct
     1801 aaaaataaaa ggtaaatcgg ttgaccttaa caaaaaatat cgtatgacaa catttagctt
     1861 taatgctact ggtggggatg gctatccacg tattgataat agacccggat acataaatac
     1921 cggatttatt gatgctgaag tacttatcga atatatccgg gaacattcac cgctggatgc
     1981 tgcttcatat gaaccaaaag gtgaagtcag ttggcagtga taacgtaatt gacgatcatc
     2041 acgtgttttt tgctgacagt cgctgtgact ttgtgaaagt agtctctgcg gatgtttgcg
     2101 atatgggaat gcagttgctg tattttgtgt ttctgcttct gcctgttgtt gctgaattta
     2161 accttgcggc ttaactgatg ttggagcgct gccagcttct tcgtcaattt cgctgattaa
     2221 agtttattca ttatggcatg atctgcgtaa gcggctcgtc agaaccgtat tgatattt
//
LOCUS       DAJVUJ010000285         2175 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.288, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000285 DAJVUJ010000000
VERSION     DAJVUJ010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.288"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            376..519
                     /locus_tag="OQQ48_004207"
                     /pseudo
     CDS             376..519
                     /locus_tag="OQQ48_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558263.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            666..934
                     /locus_tag="OQQ48_004208"
                     /pseudo
     CDS             666..934
                     /locus_tag="OQQ48_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449236.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1327..>2175
                     /locus_tag="OQQ48_004209"
     CDS             1327..>2175
                     /locus_tag="OQQ48_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCS1954741.1"
                     /translation="MIKSTNIQAIGSGIMHQINNIYSLTPFPLPMELTPSCNEFYLKA
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQEAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTHLPDLPPMLKVIHAQFNQLESLPALPETLEELNAGDNKIKELPFLPENLTHLRVHN
                     NRLHILPLLPPELKLLVVSGNRLDSIPPFPDKLEGLAMANNFIEQLPELPFSMNRAVL
                     MNNNLTTLPESVLRLAQNAFVNVAGNPLSGHTMRTLQQITTGPDYSGPRIFFSMGNSA
                     TISAPEH"
BASE COUNT          728 a          463 c          364 g          620 t
ORIGIN      
        1 gcttcttacc atatttgaaa taaagctcaa tagcgcaaat tttttgttct tcagtaaaca
       61 tgtttcatct tctgagagtc caggaaatcg tccgcatccc cattaccgtc ttatatcact
      121 ggcgctgaca tgtgctcatt gatgaagtga atctgaatga catgttttgc catatcaacg
      181 ccaaccggtg tatatttcat ttgtggagtc tccagtctga ggacgcttta tgcatcccgt
      241 attgggcagt atgatgccgg aaagctgcga ggctccacac ctgccagttt caccctttta
      301 accatcatac tactccgtgt ttaattgtgt gggatgcgtt cattacattt taacagtgtc
      361 accccgtcgg ccacgaaaga taaacaggtg accagaaaag ggatcttcat ccagcacatg
      421 ctatatcttg aacgtaacaa cctgaccctc cgcacccgca tcaagcggct ggcacgtaaa
      481 acaatctgct tctcgcgttc cgtggaaatc cacgaaaagt catcggctcc ttcattgaaa
      541 aacacatatt ctactgattg gaagcgccac ccgaaaaagt atcgtttttt tacagtaaac
      601 ttcatttagc cgactataat gtaaaaaaat aaatttttta ccgaaatata acataaggaa
      661 gtaatatgct tacacaaact atattccctt gtcttcccaa aaaacaagaa aatatcatcc
      721 ctgaagtatc aaatactata ttagtatctt ctactgttac tacagatgga tgtactgttt
      781 ttaataaaca aaaacagcca tatacgaatt gcaaatacct gctaccaacc gaacgaaaaa
      841 gttgaaattc acagctacag aaatacaatg gttgacaaag ataaatgaag ccggaattaa
      901 tgaaaaacaa agccaacggt attctgattt ctgaagttaa tttcagactc gtttaagctc
      961 cattttcaaa aaaaatatat cagggtttgt tactaataac ttctgaaaac agaaaaaaaa
     1021 atagagcagt aattctattt cagagaggag taactccatc tatcctcgat acgtactgaa
     1081 cgttatcgtg atatattcat catgctttct tcaccattat tcgagtacag ggagagacgc
     1141 aatgacctta aatacctttt tatggctgaa ttaccattct caggaataac agcagactcc
     1201 ttcccataga cggcttaggg ggaaagcaat acccggagaa agggtacaaa gcaaactgtt
     1261 ttaaaattaa taagctatat tctatttcac tagaattctt atcgctgata agaaatttta
     1321 gttataatga ttaaatcaac caatatacag gcaatcggtt ctggtattat gcatcaaata
     1381 aacaatatat actcgttaac tccatttcct ttacctatgg aactgactcc atcttgtaat
     1441 gaattttatt taaaagcctg gagtgaatgg gaaaagaacg gtaccccagg cgagcaacgc
     1501 aatatcgcct tcaataggct gaaaatatgt ttacaaaatc aagaggcaga attaaattta
     1561 tctgagttag atttaaaaac attaccagat ttaccgcctc agataacaac actggaaata
     1621 agaaaaaacc tattaacaca tctccctgat ttaccaccaa tgcttaaggt aatacatgct
     1681 caatttaatc aactggaaag tttacctgcc ttacccgaga cgttagaaga gcttaatgcg
     1741 ggtgataaca agataaaaga attaccattt cttcctgaaa atctaactca tttacgggtt
     1801 cataataacc gattgcatat tctgccacta ttgccaccgg aactaaaatt actggtagtt
     1861 tctggaaaca gattagacag cattcccccc tttccagata agcttgaagg gctggctatg
     1921 gctaataatt ttatagaaca actaccggaa ttacctttta gtatgaacag ggctgtgcta
     1981 atgaataata atctgacaac acttccggaa agtgtcctga gattagctca gaatgccttc
     2041 gtaaatgttg caggtaatcc actgtctggc cataccatgc gtacactaca acaaataacc
     2101 accggaccag attattctgg tcctcgaata tttttctcta tgggaaattc tgccacaatt
     2161 tccgctccag aacac
//
LOCUS       DAJVUJ010000286         2121 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.289, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000286 DAJVUJ010000000
VERSION     DAJVUJ010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.289"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(<1..658)
                     /gene="ipaH9.8"
                     /locus_tag="OQQ48_004210"
     CDS             complement(<1..658)
                     /gene="ipaH9.8"
                     /locus_tag="OQQ48_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HCS1954742.1"
                     /translation="MLPINNNFSLPQNSFYNTISGTYADYFSAWDKWEKQALPGEERD
                     EAVSRLKECLINNSDELRLDRLNLSSLPDNLPAQITLLNVSYNQLTNLPELPVTLKKL
                     YSASNKLSELPVLPPALESLQVQHNELENLPALPDSLLTMNISYNEIVSLPSLPQALK
                     NLRATRNFLTELPAFSEGNNPVVREYFFDRNQISHIPESILNLRNECSIDISDNPLSS
                     H"
     gene            complement(920..1015)
                     /locus_tag="OQQ48_004211"
                     /pseudo
     CDS             complement(920..1015)
                     /locus_tag="OQQ48_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1051..1356)
                     /gene="ccdB"
                     /locus_tag="OQQ48_004212"
     CDS             complement(1051..1356)
                     /gene="ccdB"
                     /locus_tag="OQQ48_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858358.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HCS1954743.1"
                     /translation="MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(1358..1576)
                     /gene="ccdA"
                     /locus_tag="OQQ48_004213"
     CDS             complement(1358..1576)
                     /gene="ccdA"
                     /locus_tag="OQQ48_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HCS1954744.1"
                     /translation="MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERW
                     QAENQQGMAEIARFIEMNGSFADENRDW"
BASE COUNT          564 a          451 c          490 g          616 t
ORIGIN      
        1 catgggatga caatgggtta tcactaatat ctattgaaca ttcattcctc agattaagaa
       61 tgctttccgg gatatgactt atctgatttc tatcaaaaaa atactctctg acaacgggat
      121 tatttccctc agaaaatgct ggtagctcag tgaggaaatt acgggtcgct ctgagatttt
      181 taagagcctg tgggagcgat ggtaaggaga ctatttcgtt atagctgata ttcatagtca
      241 ataacgaatc gggtaaagct ggcaggtttt ccagctcatt gtgttgtacc tgaagtgact
      301 ccagcgcagg aggtagcacg ggcaattctg ataatttatt gctggcggaa tataattttt
      361 ttagcgtaac aggcagttca ggtaggttag ttaattgatt atatgataca ttgagcagcg
      421 ttatctgagc tggtaagttg tcaggtagcg aggacagatt taaacggtcc agtcgaagtt
      481 catcggaatt attgataaga cattctttaa gtcgggagac agcctcatca cgctcttcac
      541 cggggagcgc ttgtttttcc catttatccc atgctgaaaa gtaatcagca tatgtaccgg
      601 aaatagtgtt ataaaaagaa ttttggggca atgaaaagtt attatttatc ggtaacatga
      661 tgggcatcca gttaaagccg gtggacaaat ggctttgtta aaagaaataa gcaggagttt
      721 ttatgaagtt cattccatga aatgaacttc agcacttttt tattataata aggataaaca
      781 aaattggctg taaaaaaacg atactgtttc agttaaccgt gaatggcctg tttgcaccgt
      841 attgttcttg tttttcggtt agttaaacat cgtacttcat atttgaacgg taagcggctc
      901 gtcagaaccg tattgatatt tactgagagg tcagatcaac tttccagggc aacagatcgc
      961 gtacccggtt tgccggccag tcttggatat gctcaatgac gtaacgcagc cattttgtca
     1021 acgccacgat ttttgaccct agcctgacat ttatattccc cagaacatca ggttaatggc
     1081 gtttttgatg tcattttcgc ggtggctgag atcagccact tcttccccga taacgaagat
     1141 cggcacactg gccatatcag tggtcatcat gcgccagctt tcatccccga catgcaccac
     1201 cgggtaaagc tcacgggaga ctttatctga cagcaggcgg gcactggcca gggggatcac
     1261 catccgtcgt ccgggcgtgt caataatgtc actctgtaca tccacaaaca gacgataacg
     1321 actctctctt ttataggcgt aaaccttaaa ctgcatttca ccagtccctg ttctcatcgg
     1381 caaaagagcc gttcatttca ataaaacggg cgatctcagc catcccctgc tgattttcag
     1441 cctgccagcg ttccgcacgc agacggcggg cctctttttg catggccgta tttaccaggc
     1501 ctgagatatt cacgtttgcg gatttaagta actgatagct gtcactgtct atagtgacag
     1561 taatacgctg cttcatgata agccctcaaa aacatactta tgatatgtat atgagtatat
     1621 attaatggta caaaccatca atatccataa aaagtacacc attaattcac tataagtttt
     1681 taatacaatt ttttctgctg cgattttttt atacgataat tagcatactt ataacaccgt
     1741 aagtaaatta catgaaagca ggcacagcgt gatccgattt ttctgcgtat aacgattttc
     1801 cggaagtgta atacggttgc gccccgggcc gggcaccctt ttctgttgtt gtgtacgggc
     1861 ccacagagaa tggtaccgga ggcgggctca gaagtactga gtcgggtgat cgcgtcaggc
     1921 cgcgaagtac tgacacgggt attggtgact ggtgatgaag tactgccccg ggtatgattt
     1981 caggctgatt caggttctga ttctctgtca gtccgcgtgg ttgctggcct ggctgatttc
     2041 ctgttgtcag gcgttcaaca accgactgca ggttatagcc cggttcacga accacggcag
     2101 cctggcggct gcaaatcggg g
//
LOCUS       DAJVUJ010000287         2091 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.290, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000287 DAJVUJ010000000
VERSION     DAJVUJ010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.290"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(<1..163)
                     /locus_tag="OQQ48_004214"
     CDS             complement(<1..163)
                     /locus_tag="OQQ48_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inhibitor of g-type lysozyme"
                     /protein_id="HCS1954745.1"
                     /translation="MKIKSISKAVLLFALLTSTSFAAGKNVNVEFRKGHSSAQYSGEI
                     KGYDYDTYTF"
     gene            405..698
                     /locus_tag="OQQ48_004215"
     CDS             405..698
                     /locus_tag="OQQ48_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HCS1954746.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            770..1429
                     /locus_tag="OQQ48_004216"
     CDS             770..1429
                     /locus_tag="OQQ48_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HCS1954747.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            1521..1967
                     /locus_tag="OQQ48_004217"
     CDS             1521..1967
                     /locus_tag="OQQ48_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HCS1954748.1"
                     /translation="MNDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PVWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
BASE COUNT          546 a          451 c          543 g          551 t
ORIGIN      
        1 agaaggtata tgtatcgtaa tcgtatccct ttatttcgcc ggagtactga gcgctgctat
       61 ggccttttct gaactcaaca ttgacattct tacccgcagc aaagctcgtg gaggttagca
      121 gtgcaaacag caatacagcc ttactgatgc tcttgatttt cattggtagt ctcctgttca
      181 gaatgagtac gatgaactgt tataatataa caatccctaa cgggagaatt ccagtggctt
      241 ttctgcgttc ggtaaaatcc cggcgcggca ttttttgccg gggggattac ttataaaggg
      301 gcatggactc aacaaaccgc agaaatctac agctggctgg cttgtggcgc gggtttcatt
      361 gatgttagct tatgccaaaa cctggcatct taaagagtaa aagtatgttt tgtgtgattt
      421 atcgaagcag caagcgtgac cagacctatt tatatgtcga aaaaaaagac gatttttcgc
      481 gtgttcctga ggaactgatg aaaggttttg gtcagcctca gttagcgatg attctgccgt
      541 tggatgggcg gaagaaactg gtcaatgctg atattgaaaa ggttaaactg gcattaaccg
      601 agcaaggtta ctatttgcag ttaccgccac cacccgaaga tttgctgaag caacatcttt
      661 ccgtcatggg gcagaaaaca gacgacacga acaaataacc gatatccggc ggtggcatta
      721 tctttgtcgg cgcgggtttt catatccacg ataaggtgag gggaacgtta tgtatcaaca
      781 tcacaactgg caaggtgcgc tgctggatta tccggtgagt aaagtagtct gtgttggcag
      841 taactatgcc aaacatatta aagagatggg cagcgcagtg cccgaagagc cagtgctgtt
      901 tattaaacca gaaacggcac tgtgcgatct gcggcagcca ttggcgatcc catccgattt
      961 cggttcagtt catcatgaag tcgaactggc ggtgttgatt ggtgcgacgc tgcgtcaggc
     1021 aacggaagag catgtccgca aagccattgc cggttacggc gtggcgcttg atctgacatt
     1081 gcgtgatgtt cagggaaaaa tgaagaaagc cgggcagccg tgggaaaagg ctaaagcgtt
     1141 tgataactct tgtccgcttt ccgggtttat ccccgcggcg gaatttaccg gcgatccaca
     1201 aaatacaacg ctgggcctga gcgtaaacgg cgaacaacgc cagcaaggta cgactgcgga
     1261 catgatccat aaaatcgttc cgttgatcgc ttatatgagc aagtttttta ccctcaaggc
     1321 cggtgacgtt gtgttgacag gcacgcctga tggcgtcggc ccgttgcaaa gcggtgatga
     1381 gctgacagtc actttcgatg ggcattcttt gacaactcgc gttttgtaat actttttgcc
     1441 gcctgaaagc ggcggcaaaa cttgcatcgc tgtgccagac tggttataag gtgcgtttta
     1501 acgtaatggc ggaacacctg atgaacgatg tacctttctg gcaaagtaaa accctggacg
     1561 aaatgagcga tgcggaatgg gagtcgttgt gtgatggttg cggtcagtgt tgcctgcata
     1621 aactgatgga tgaagacacc gacgaaatct acttcactaa cgtcgcctgt cgccagctca
     1681 atattaaaac ctgtcaatgt cggaactacg aacgtcgttt cgagtttgaa cccgactgca
     1741 ttaaattaac ccgtgaaaat ctgccaacat tcgaatggct gccaatgacc tgcgcttatc
     1801 gtttgctggc ggaaggtaaa gatttacctg tgtggcatcc gctacttact ggttcgaaag
     1861 cggcaatgca tggtgaacgt atctctgtgc gtcatatcgc agtgaaagaa tcagaagtga
     1921 ttgactggca ggatcatatc ttaaataaac ctgactgggc gcagtgataa atttaataga
     1981 tgttgatggg ggctttctta atatacgtct ggatgctcgc catttcgtag ccctgttttt
     2041 gaaagcgctc tgtcataatg agagttaccc gataccttat tatcaacagg t
//
LOCUS       DAJVUJ010000288         1820 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.291, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000288 DAJVUJ010000000
VERSION     DAJVUJ010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.291"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            70..391
                     /locus_tag="OQQ48_004218"
                     /pseudo
     CDS             70..391
                     /locus_tag="OQQ48_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232281.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(425..631)
                     /locus_tag="OQQ48_004219"
     CDS             complement(425..631)
                     /locus_tag="OQQ48_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1954749.1"
                     /translation="MTVSYKITYEHRDGDQIRMEEVLFHSEHEPTKDEIHDAVRKDTV
                     RFNSPSNGVTAVSGFKVISVVPVP"
     gene            complement(1129..1202)
                     /locus_tag="OQQ48_004220"
     tRNA            complement(1129..1202)
                     /locus_tag="OQQ48_004220"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1168..1170),aa:Gly,seq:ccc)
     gene            complement(1281..>1820)
                     /locus_tag="OQQ48_004221"
     CDS             complement(1281..>1820)
                     /locus_tag="OQQ48_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCS1954750.1"
                     /translation="PYTIEVGQKLKLGGAKSSSSTRKSTAKSTTKTASVTPSSAVPKS
                     SWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYAAGAGKVVYVGNQLR
                     GYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKTGQKIATMGSTDAASVRLHFQIRYR
                     ATAIDPLRYLPPQGSKPKC"
BASE COUNT          479 a          422 c          402 g          517 t
ORIGIN      
        1 ctaagttaat gcattaatct aaaaacgctt taaatcaata cataaccatt atctcttggg
       61 attatttaca tgcgcaaaag tttaatgctg ttacttgctt caacattgat tatttctgga
      121 tgtgcaacaa aaaaacaact cgttccaact ggtggaagca aatcagacgg cacagttagg
      181 atgagttatt cttacggtat gtttgaaagt cctgttgtag atccacaaca aggcatgaat
      241 gcagccaaag cgcgttgtgc cgcttagggg atattcagga gccgagcctt ttggtggatt
      301 cacttctcaa tgttcacaac cttcttcatc tggttgtatg caaacacttg tgactatcga
      361 atatcaatgc actggcgata ttaagaagta ataattaggg gcttccagcc ccttctataa
      421 ccattcatgg aacagggaca accgaaatca ctttaaaccc tgaaacagcc gttactccat
      481 tagatggaga attaaatctc acagtatctt ttcttactgc atcatgaatt tcatcttttg
      541 ttggttcatg ctctgaatgg aataaaacct cttccattct aatttgatca ccgtctctat
      601 gctcataagt aatcttgtag ctaacagtca tatcaacctc atgcagggga attgtgagat
      661 tattatagac aatagaaacc ataaagcccc gttggtgata cagcaacaag cgggagcgta
      721 gcgacttcat cagattgtca gaaagatcac accttatcag gataaccgtg tacccgttgg
      781 gagcgtggct gaaacgctga tagagcttgc atatggtttg caaggaccaa tatcaccata
      841 aacctgttta cgcagccaca aggccgcaca tcgggcttaa atgcgtgtgt tcagtctgtc
      901 gatgacatta caatgccagg atgggcttta tcttcacgtt gtgctatcgc ggtgtgagag
      961 gtgcaactgc cgccaacgtt aggttatact gtcatctcac cgtgtaactt aacgtgacag
     1021 ttactaaggg cggatcacaa tggtagaact cctaagcaaa ttaaataggt aactgtttaa
     1081 gttgctgaca gagaagactt ttttagtctt ctccacctgg ggagattctg gagcgggcga
     1141 agggaatcga accctcgtat agagcttggg aagctctcgt tctaccattg aactacgccc
     1201 gcttcgagat gcgtaaggca ttataaacct tacgctctcc ttagcaagtg ccgcgctgct
     1261 gactgctgat taattcgcca tcagcatttt ggcttgctgc cctgcggcgg caagtaacgt
     1321 agcggatcaa ttgccgttgc acggtaacga atctggaaat gcaggcgaac agatgccgca
     1381 tccgtgctcc ccatagtggc gattttttgc ccagtcttca cgctttgccc attatttacc
     1441 agcatcgtgt cattatgggc gtaagccgta atgtaatctt cactgtgttt aatcatgatg
     1501 agattaccgt agccacgcag ctggttgccc acatacacca cctttcctgc acccgcggcg
     1561 taaataggtg taccccgtgg agctgagata tcaatccctt tattgccgcc atctgctgtc
     1621 gaatacggca tgataacttt ccctgtcgtt ggccataacc aacaacgttg ccctactggc
     1681 ggccaggaag atttcggtac cgctgatgac ggtgtaaccg atgcggtttt ggtcgttgat
     1741 ttggcggttg atttacgtgt actactgcta cttttcgccc cacccagttt tagtttctga
     1801 ccaacttcaa tggtgtaagg
//
LOCUS       DAJVUJ010000289         1806 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.292, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000289 DAJVUJ010000000
VERSION     DAJVUJ010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1806
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.292"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(306..1454)
                     /locus_tag="OQQ48_004222"
                     /pseudo
     CDS             complement(306..1454)
                     /locus_tag="OQQ48_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT          574 a          463 c          389 g          380 t
ORIGIN      
        1 gttattaaca acgttgccaa aaccccagac agtgataaaa gacatcagcg caatgttgta
       61 ccatcgcagc tgtttttcct gaacattccc agccatagac aaatccttcg ccaaaattat
      121 aggggcatct gtaaaacgag ggattatgcg cggcaaataa gaagtgcgga aatagcgaaa
      181 tgcaaaatta taacctgctg cacattttat ttttaaacga ataaaatttg gaataaagca
      241 agtcagatag aagaaacaaa aaagcagagt gtgaaacagt aagttcacac tctgcttgaa
      301 ttcatttaca cgtgagcaac gggtttcggc gtatgaccgg cgttgcgcag cagcatcagc
      361 aaatagataa acgatacgct ggcgatcatg ataaacaata aattatcgga gaaattctgc
      421 attagcatag cggtaaatga cgggccaagc agacttccca cagtatagct caacagtaag
      481 gcctagttca tcgccaccag ttgatgatgt tcaactttct cgcaagccca tgccatcgcc
      541 accggatata gcgtaaagcc agcggcaccg aggatgaata acgctggggc catcgccgcc
      601 tggctaagca tcgcgatact gccgagaatg acgacaaaga cctgaacacg caacaccagc
      661 agtcgaccaa acttatccgc cagacgtcca atcgaccatt gtccaaggat acccgcactg
      721 accagtaccg ccatccagaa accaatgctg gcattgctca cccctttgtg attgaggtac
      781 agcggcatca ggccatatag agatcccaga acgattcctg agataatgca gccattcacg
      841 ccaagccgcg cctgacggag ttttagcatt gacgtaattg acgtcgaatc atggttttca
      901 gcctgctgat tcagcacacg cgtaaacaac agcggtaaga tccctgccaa cgtcaaacct
      961 gtaacccacg gcaatacgga catcagctcg gttgaaactt tgctgaccag taactggcct
     1021 aaaaacgttc ccacgtaata aaccatcata tacgcagcaa gcaaacgccc acggttacgt
     1081 gacgtcccac tgcacatcag cgcgctctca acaaccaccc aaatcatggc acagccgacg
     1141 cccgcgacaa aacgccaagc caaccagctc cagaatccaa tcatcaggcc aaggccggca
     1201 cagccagcgg caaaaatgaa ggaggccaga taatagctgc ggttaaagcc aatgcgctta
     1261 atgacatacc ctgtcagcaa tgtaccgaca aggttgccgg taaaatagga tgagctgaca
     1321 acgcctacct gccatgtgga catgtgttcc tgggcgagcc aaagcggcac gagtgtattt
     1381 aacaccgcaa tcgccagagt caacaaaagc aggccagaca gcaaaagcat gacaggccgg
     1441 gtatacgtgg acatgaatta aaaccgtgag gaagttcaaa tttcatgcgc atcatgccac
     1501 cgccaaaaca ttgtcaatga gtgaaaatac gcgatatccc agcggcggta ttatcgattt
     1561 atattactca ttcataacgc taatgctgaa gagatgatta gtctcatctg attgttttta
     1621 ttgaactaac acaaatttca tacgcttcgt tcagtcgaaa aatttcatgg tcacagcaaa
     1681 ctcatatttc ccgtctatgc tttcagagca atgcatcgcg tctgatcgat ttcagacgta
     1741 ccactgactc tcgaaaggag aagaggatga ccaaaccgta tgttcgtctt gataaaaatg
     1801 atactg
//
LOCUS       DAJVUJ010000290         1758 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.293, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000290 DAJVUJ010000000
VERSION     DAJVUJ010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.293"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..277
                     /locus_tag="OQQ48_004223"
     CDS             <1..277
                     /locus_tag="OQQ48_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="oxidoreductase, Fe-S subunit; terminal electron
                     transfer protein for the reduction of DMSO; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase subunit B"
                     /protein_id="HCS1954751.1"
                     /translation="QYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDEL
                     RKKHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            279..1142
                     /gene="dmsC"
                     /locus_tag="OQQ48_004224"
     CDS             279..1142
                     /gene="dmsC"
                     /locus_tag="OQQ48_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HCS1954752.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLIVTMVLGVIFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVVSVMQGAELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(1177..>1758)
                     /locus_tag="OQQ48_004225"
     CDS             complement(1177..>1758)
                     /locus_tag="OQQ48_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCS1954753.1"
                     /translation="LLVDHQAGLLSLVRDIEPDKFKNNVLALGDQAKYFNLPTILTTS
                     FETGPNGPLVPELKAQFPDAPYIAHPGNINAWDNEDFVKAVKATGKKQLIIAGVVTEV
                     CVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGAQLMTWFGVACELHRD
                     WHNDIEGLATLFSNHIPDYRNLMTCYDTLTKQK"
BASE COUNT          377 a          422 c          489 g          470 t
ORIGIN      
        1 gcaatataac gaaacgaaag gccatatgac caaatgcgat ggttgttatg accgtgttgc
       61 ggagggtaaa aagccgatct gtgttgaatc ttgtccgctg cgggcgctgg atttcggccc
      121 tatcgacgag ctgcgtaaaa aacatggcga tctggcggcc gttgcgccgt tgccgcgagc
      181 tcactttacc aaaccgaata ttgtgatcaa acccaatgcc aatagccgcc cgaccgggga
      241 taccactggc tatctggcaa acccgaagga ggtgtgagat gggaagtgga tggcatgaat
      301 ggccgctgat gatcttcacg gtcttcgggc aatgtgtagc aggtggtttt atcgttctgg
      361 ctttggcgct gctcaaaggc gacctgcgag cagaagccca gcagcgtgtt atcgcctgca
      421 tgtttggttt atgggtgctg atgggcattg gctttatcgc ctctatgctc catcttggtt
      481 caccaatgcg cgcttttaac tcgctcaacc gggtaggggc ttcagcactc agtaacgaaa
      541 tcgccagcgg ttcgatcttt tttgccgtag gcggcatcgg ctggttgctg gcaatgctga
      601 aaaagctgtc accggcattg cgtacgctgt ggctgatagt gacgatggtt cttggcgtca
      661 tctttgtctg gatgatggtg cgtgtgtata acagcattga taccgttccg acctggtaca
      721 gcatctggac gccgatgggc ttcttcctga cgatgtttat gggcggcccg ttactgggtt
      781 acctgttgtt gagtctggct ggcgtcgatg gctgggcgat gcgtctgctg ccagcaattt
      841 ctgtactggc actggtagtg agtggcgtgg tgtcagtgat gcagggcgca gagctggcga
      901 ctattcatag ttctgtgcag caggccgcag cgctggtgcc ggactatggt gcgctgatgt
      961 cctggcggat cgtgcttttg gccgttgccc tgtgcttgtg gattgcacca cagctaaaag
     1021 gttatcagcc tgcggttccg ctactttctg tatcattcat tctgcttctg gcaggggaat
     1081 taatcggtcg cggcgtattc tatggtttgc atatgaccgt ggggatggcc gtcgcaagct
     1141 aatcataaca accggggttt cggccccggt tctcttttat ttctgcttcg ttaacgtgtc
     1201 ataacaggtc atcaaattac gataatcagg aatgtggtta gagaacaacg tcgccagtcc
     1261 ttcaatatca ttatgccagt cgcgatgcag ctcgcacgcc acgccgaacc atgtcatcaa
     1321 ttgcgcacca gcttgcgaca tgcgatccca tgccgaatgc cgggtaattt cattaaaggt
     1381 ccctgacgcg tcagtcacga caaagacgtc aaacccttct tcaatggctg aaagcgccgg
     1441 gaatgccacg caaacttcgg ttaccacacc ggcaataatt aactgttttt tacctgtcgc
     1501 tttgacagct tttacaaaat cttcgttatc ccaggcgtta atatttcccg ggtgagcaat
     1561 gtaaggtgca tcggggaatt gtgctttcag ttctggaact aatgggccgt taggaccggt
     1621 ttcaaaactg gtggtaagaa tagttggtaa gttgaaatac ttagcctggt cacccagcgc
     1681 cagcacgtta tttttaaact tatcgggttc gatatcccgt acaagggaaa gtaaaccagc
     1741 ctggtgatca acaagcaa
//
LOCUS       DAJVUJ010000291         1730 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.294, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000291 DAJVUJ010000000
VERSION     DAJVUJ010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.294"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(855..1328)
                     /locus_tag="OQQ48_004226"
                     /pseudo
     CDS             complement(855..1328)
                     /locus_tag="OQQ48_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708890.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase
                     domain-containing protein"
     gene            complement(1256..1622)
                     /locus_tag="OQQ48_004227"
                     /pseudo
     CDS             complement(1256..1622)
                     /locus_tag="OQQ48_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          386 a          486 c          417 g          441 t
ORIGIN      
        1 tcgtcaaaaa aaatcatctt cgtgaagttt tacgacatag tggaggagtt tgcttctttg
       61 aggtgcaggt tttcgtttgc gtggtttagc tgaacgggcc atataactac ctgaaagaca
      121 atgatattgt ctgtttttat aacggtaatc acaggccatg acaagccgca gccgtcaggc
      181 cgcctactcg ggtcggttta gaacgcttat ttagtaccag atcttactta taataaaggg
      241 gcttggcagt ttggataaac tattgtcctt attccactaa tacaaacttt ttgtaaagta
      301 tattaaatat acttcatttc atactgaaat ccccggaagt attcaatcaa acaattacac
      361 cataaaagga agagggggcc ccaattaagt tgtcaagcat gtttactgcg caatcgcttg
      421 gtgctccgtc cctgtacccc gcattggtac tctggcgctc atggggcttc cacttgcgct
      481 tctcccttca catcggggtg acaggtccac gttccccaca agagcctgaa tcgacttcac
      541 gccgctttta tgccggatgc cgcgcaggca gtaaacatat cccacaggaa cgactccacc
      601 attgttttga catcatctat atgctttcga cacatcatca gcggttcact ttcgttcgtc
      661 tcttcgaacc atacctgatc ggctcacgtc cgaccttttc ccatctcgct caccaccaca
      721 tctcttaaat gcagcagcag tggatggttt ggtgcctgcg cctgcacgcc gacaccgagg
      781 ggccttccct catctcttgt gccgcatggc tgcacaggga ctcacgtccc caggacgcgc
      841 cttcgtggca cacagtcatc ggcatagcaa cagtaagcaa cggcaggttt ccattgccag
      901 ttttctttga cagcagtact tcggcctcgc tggatactgt gattccagta ccagcgatcc
      961 tttctggctt ttccactcag gtagcgttta ttcagatact ggtcgaactc attcagtatg
     1021 atgttcgaca gcaacggcga gatcacaccg ccctgtggca tactttcact gaccgcccgg
     1081 aagagaccaa catcaacacg gcgttttcca cagcagtctc ataaaacgtg catcactgat
     1141 cctgcggcag acggctttca tcagcagccg gtgatgtact gtgtcgaagt agctggacag
     1201 gtcaccttca ataacccggc gccccgtgtt tccccgcagt cggtgagctg taatagtacg
     1261 tgatgtctcc cgcccacttc tggttcgggc cactggcgta aaaatcctgc gtcagcagat
     1321 tttctgatac aggcaggccg tgtgcacggt aactgatcgg gctgaacttc tgggaggctt
     1381 ttgcgctcag ttcctgacgg cgcagactgg ctgccacggt ttttacgtta aagtggtaac
     1441 cagagcacac agttcatccg tcaggcgtgg ggcaccgtaa cgctggactg ggtaaaagcc
     1501 ttgcggacaa cgctgtcgca gtgctgacgg aactgctgac gtgtgtttat ccttgtccgc
     1561 cgctgacacc acgtatacca gccgctgcgg gccacccgga gtacgcggca cattgccttg
     1621 atgacggttt acgcggtcgc ctgcggcaac ggctttccgg ctgacggtag tgtggacctt
     1681 tttaccccgg agaacaccgg caagccccat aaccgccatg agacgtgcca
//
LOCUS       DAJVUJ010000292         1658 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.295, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000292 DAJVUJ010000000
VERSION     DAJVUJ010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.295"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..198
                     /locus_tag="OQQ48_004228"
     CDS             <1..198
                     /locus_tag="OQQ48_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HCS1954754.1"
                     /translation="SLVSRIPGAVQNEWESAFVELQKMFSLTQRDNAMLNEQVMQLSQ
                     QVQHLSERTERLSQLYSENRR"
     gene            complement(747..1214)
                     /locus_tag="OQQ48_004229"
     CDS             complement(747..1214)
                     /locus_tag="OQQ48_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014526575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1954755.1"
                     /translation="MRIDISHQTRHTPPNMLPREQNCVAMALSACFRQQLNPVVNSLL
                     KERIIHSPKELEHDNAVIRALQKLQIQEVCNSTLWETAKQQLLQKSDGRYFAINSKHL
                     AFPGPGESHAFCCIKYKNAIGINGNNAETQSTHYQPYPYDKVSIWGPFPHNLT"
BASE COUNT          386 a          358 c          440 g          474 t
ORIGIN      
        1 agtttagtct ccaggattcc cggggcggtt cagaacgaat gggagagcgc cttcgtggaa
       61 ttgcagaaaa tgttttcgct tacgcaacgg gacaacgcga tgctgaacga gcaggtcatg
      121 cagttgagtc agcaggtgca gcacttgagc gagcggacag aacgcttgag ccagttatac
      181 agcgagaaca ggagataagg gacgaacggc tgatacagga atgcgaacat gagttatccc
      241 tggagcgtga gcgccagccg gaaatacagg aacgcacact ggatggtcct tcactgggat
      301 ggtaatcaga gacaggctaa aatgttgtca gcgcgtttcc ggtgcatttt ttgggataat
      361 ggttggttcg gggggggcac cgggaacgcg cttcagtcag accttgctga aaacacagga
      421 acgcgttaaa acgcatacgg gcgcgattct ggtgtgtctt ttgttgctgt gttttgcctc
      481 tattgccaga tcgcgctcat tcccctgaac ttctgttttc ccgctcaggt ccccgtcttc
      541 ttcgcttccg aaagcatgaa aaaaatccgt ttcagctgtc tctggctggc aattcctgcg
      601 tgattcacat ggctgcatag ctatgcatgt ggttaaaatt taccgggtgc gatcgcggca
      661 gtttttcggg tggtttgttg ccatttttac ctgtctgctg ccgtgatcgc gccgaacgcg
      721 tttcagcggt gcgtacaatt aagatattac gttaaattgt gagggaatgg tccccatata
      781 ctgactttat catagggata gggttggtag tgggtcgatt gggtttcggc attgtttccg
      841 ttaatgccaa ttgcattttt gtatttgatg caacagaagg cgtgagattc tcctgggcct
      901 ggaaaggcta agtgtttaga attgatggca aaatatcttc cgtccgattt ttgaagtagt
      961 tgttgtttgg ctgtctccca aagggtgctg ttacaaactt cttgaatttg tagtttttgc
     1021 agggctctta ttactgcgtt gtcatgttcc aattctttag gggagtgtat tattctctct
     1081 tttaataagc tgtttaccac agggtttagt tgttgtctaa agcacgcaga taatgccatt
     1141 gctacacaat tttgttcccg aggaagcatg tttggcgggg tatgccgtgt ttgatgtgat
     1201 atgtctattc tcatagaagg tctctggctt atagcatcaa caaatacggc tataaaacaa
     1261 aattttcata gagtgattca agatcttctt gagatccttt tttgcggcgc gtaatctgct
     1321 gcctgcaaac aaaaaaacca ccgctaccaa cggtggtttg tttgccggaa tctagagcta
     1381 ccaactcttt cttccgaagg taactggctt ggtggagcac agataccaaa tgctgtcctt
     1441 ctagtgtagc cgcagttagg ccaccacttc aagaactctt aatatctcaa tccaccttgt
     1501 acagttacca atggctgctg ccagtggcgt taagtcgtgt cttaccgggt tggactcaag
     1561 acgatagtta ccggatgagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     1621 cagcttggag cgaacgacct acaccgaact gagatacc
//
LOCUS       DAJVUJ010000293         1653 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.296, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000293 DAJVUJ010000000
VERSION     DAJVUJ010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.296"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(2..952)
                     /gene="corA"
                     /locus_tag="OQQ48_004230"
     CDS             complement(2..952)
                     /gene="corA"
                     /locus_tag="OQQ48_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HCS1954756.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            1322..>1653
                     /locus_tag="OQQ48_004231"
     CDS             1322..>1653
                     /locus_tag="OQQ48_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1954757.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFSVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQ"
BASE COUNT          429 a          427 c          413 g          384 t
ORIGIN      
        1 attacaacca gttcttccgc ttaaagtaca gatacggtgc caggcccgcg aggatcataa
       61 agataatcgc gccagggtag ccgaagctcc acttcagttc tggcataaac tcaaagttca
      121 tgccatagct ggaagcaacg agcgtcggcg gcaggaatac cacggatacc accgagaaga
      181 ttttgatgat gcggttctgc tcgatgttga taaaacccat cgccgcctgc atcaggaagt
      241 tcaccttctg gaacagagat tcgttatgcg gcagcaggga ttcgatatct cgcaggattt
      301 cacgcgcctg ctccagttgt ccacccggta agcgtgcttt acgtaccagg aagttgagcg
      361 cgcgctgggt atccatcaga cacaggcgta ctttccagcc gatatcttcc agttccgcca
      421 gcgtggagag cgcctcatcg tactcatcgc cctgatgccc ttccataatc acccggctca
      481 actgctccag gtcgctatag atattttcaa tttcatctgc caactgttcg attttggttt
      541 cgaacagatc cagcagcagc tcgtaggcgt taccgtctac catcgactgg ctacgggcac
      601 gcatacgata cagacgaaaa gcgggcagtt cacgctcacg cagagtaaac agacgaccat
      661 cacggatggt aaatgccaca gtggagttac cggcgtgatc ttccgcatct tcaaagaaga
      721 agaaggagtg aatatgcagg ccgtcgtcgt cttcaaagaa acgtgccgat gcttcgatgt
      781 cttccagttc cgggcgggtt gccaggctct ggccaagttc agattgtaca cgcagtcgct
      841 cgtcgtcgtc cggttcgaca agatcaatcc atactgcatt tacaaggggt tgtgactctt
      901 cgacttccag ccgggtcagt cggttatttt ccagttgaaa tgcgctcagc atgaccggga
      961 ctcccaatgc ttaaaatatc ggacagttcg gtgggcacac agaaacaaat tgggtttcag
     1021 accattaaac agcctgactc agcgcgacgg gaaaaattga ggtcgctgac aaccgctaag
     1081 gctatcagca aaaagggata gccttaggag ttgatcctgg atgacaggat agtgagccag
     1141 tatctactgg gtgtgtccaa ggcgaatgtc ctcttagaat gatcgtgcgc gcatgttacg
     1201 ccagcaaaat tttgccgtca acacgcaaca gaacaccaca gaacaatagt tgaacttcgc
     1261 gctttttggt actcctcaag gtcgctaaca ggataaaaaa atcccacatc caggacctat
     1321 aatggcgcat cggctactca ttggtaaagg aatgatcacc ttgaatctca aacggatttt
     1381 tctcaccctc accttacttc ccttgttttc tgttgccgct gatgattgcg cactctcaga
     1441 tccgacgctg accgtacagg cgtataccgt taatcctcaa acagagcggg tgaaaatgta
     1501 ctggcaaaaa gccaatggcg aagcgtgggg aacgttacat gctctgctgg cggatattaa
     1561 tagtcagggt caggtgcaga tggcgatgaa cggcggcatc tatgatgaaa gctatgcgcc
     1621 gctcggtttg tacatcgaaa acggtcagca gaa
//
LOCUS       DAJVUJ010000294         1589 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.297, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000294 DAJVUJ010000000
VERSION     DAJVUJ010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.297"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(343..498)
                     /gene="hokD"
                     /locus_tag="OQQ48_004232"
     CDS             complement(343..498)
                     /gene="hokD"
                     /locus_tag="OQQ48_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCS1954758.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(1150..1566)
                     /locus_tag="OQQ48_004233"
     CDS             complement(1150..1566)
                     /locus_tag="OQQ48_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1954759.1"
                     /translation="MTELTKEQLIEEAKLKIAITKCHPNSGMARVEGELFKIARASLE
                     AEPIAWRYRYVKKGVMDSQGELWVGDWKYVPKKEDCNDRPNYEIQALFTAPPVPVTSE
                     ELVKAVHFYEQLKRENPPASGNQINGLTMSVKRPAN"
BASE COUNT          387 a          404 c          333 g          465 t
ORIGIN      
        1 ctttcttaat ctcgtatccg tgtctgcggt agcgctgaat cagccattca gcctgttctt
       61 cggtgcaggg gtcgtgctga taccagtcag atttgaatgc atgagaacgc cgcccgtgcc
      121 tgctggcagg cgcggcagag ttatccgaat tgtaaaattt ggtatcgtgc gccatctgtt
      181 ttctctgctg gcgcagcagg tgccagttgt tcaggctgac ggatggattg taaaccagaa
      241 cgaccagaaa aaacaaaacc cgccgaagcg ggttaagtgc gggtgcgttg aggatgcctg
      301 acacatcagc ggtggcgagg gatttctccc ccgccgggtc tcttactcct caggttcgta
      361 agctgtgaag acagcgacct ccgtctggcc ggttcggatt cgtacctcgc agaggtcttt
      421 cctcgttacc agtgccgtca ctatgacggt taaacagatg acgataaggg cgattaacat
      481 cgccttttgc tgcttcatag cctgcttctc cttgcctttc ggcacgtaag aggctaacct
      541 acatgtgttc agcatggatt gagcctcaga ttaatgttaa gcgtcttgca ggacgcgtag
      601 tgttaactgg ggcttttctc tatctgcctt ttggtgttca tgcctgagac agatagcctc
      661 aagcacccgc agtcattcta cttaactaag atttccctgc aaaccgtttt tgtccggcac
      721 agtaaatatc caactaaacc aatagcgttc gctgtattta ccgccagtat tcaatgcaca
      781 tgaccgccat gaacacccct aaaaaaaggg catttatatg tccaaacatt aatatcaaaa
      841 catcaatttt ttccatatac cttgctgtga agatgatggg catacatgat gcgaacaacc
      901 agaacgcaac aaacaaaaac tgcaatgcgt ttttcattat tccccctaca atcaatgtgc
      961 aataacattt aaacacacct caatttggcc ggacatataa atatctaaac cagaaaaaat
     1021 cacttacata gcgttacaaa ctctttagtc taaatactca tcgtaaaaca ttccccatac
     1081 ttatcagtcc gttccgcgcc aggtagctta ttgccttatc tggcaaccta taatcaggtt
     1141 tccgcttttt cagttggctg gtcgtttaac cgacatagtt aacccattaa tctggttgcc
     1201 ggatgctggt ggattttcgc gttttagttg ttcataaaag tgcacagctt taaccagttc
     1261 ttctgatgta accgggactg gtggggcagt gaataaggcc tgaatttcat agttcggcct
     1321 gtcgttacaa tcctcttttt tcggtacata tttccagtca ccaacccaca actccccctg
     1381 agagtccata acaccttttt tcacgtagcg atatcgccac gctatcggct ctgcttccag
     1441 cgatgcccgt gcaattttga ataactcgcc ctctactcgc gccatccctg aattggggtg
     1501 gcatttcgta atcgctattt ttaatttggc ttcttcgatt aattgttctt ttgttaattc
     1561 agtcattttc attaccgccc tttcaggcg
//
LOCUS       DAJVUJ010000295         1575 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.298, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000295 DAJVUJ010000000
VERSION     DAJVUJ010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.298"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(76..1575)
                     /locus_tag="OQQ48_004234"
                     /pseudo
     CDS             complement(76..1575)
                     /locus_tag="OQQ48_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin acylase family protein"
BASE COUNT          400 a          404 c          345 g          426 t
ORIGIN      
        1 ttctgttcgc catgatgcgc gacgggactt tttatacccc gcaggggtca taacatgctt
       61 gacaacttaa taggggcccc cccacaaaaa ggcaaacaga tccgaagcgg gataatcttt
      121 ttggggagaa ttgttccagt tagcaatata tcccgacagg gggttataca ccttagggtt
      181 catttcaaaa ggcaatagcc ctttccagtc ccattttccc gtaccaggaa cgggtaatcg
      241 cggatcatgg cctgattgac gatctggata agcaccagta tgaacataac caatattgcc
      301 gtttacatca gcatagtacc agttgatggt cagtgcttgt ttcgctactc ctgccaattt
      361 ttggccttca tctgatgagt ccaggccagc aaagacgcca cctctttacc atcccatgcg
      421 cgggatttag cgtaagccgt ttgtgtcgtc tggtcagttt ggagaatgtt gccatgcacc
      481 gtacgccaga cagtaaaggt ctctgcctga ccatttttca ccgtaatggt ttcctcacgg
      541 cttaacattt tcacccactt acaattatgt aagtagtagc ctggtttctc tgccgacagc
      601 cgttcagcaa aaatatcgac atcatcgccg aaacctgccg ttgatcccca ggaaatcaca
      661 ccattatgac caaaaaccag cccaggatag gcaaatggtg tattgccagt gacatcataa
      721 ccagcaccgt gcagaccaat accataagta tacgcaggcg cataccagcc aaactgcgga
      781 ccatttacca tgattgcttt cgcatcctgg gctttgcttt tgccgatcac ccacatattg
      841 ctggtcgttg gataccccgc cagaccattg gcaccaccct gtgcaaattg tgcagcaata
      901 gtttcccggt tcttccctgc tgttaacgcc agcagtgcgc catccgcccc ttttgctggt
      961 cggtcaagca ttggtgcagg taaatcgtag cgtggcaaca gagctgctgt ttgcgagttt
     1021 tgctgattaa atttaagtgg gtagctactc tcttgtacgg caatagtggt tggcgctgat
     1081 gggtttacca gccatttcaa ctgattaaat accgccatgc cttgtgatac accatattta
     1141 tcttttaaag ccgttagcag tgccagatta tcaatttcgc tagtgctatc agagaagcgg
     1201 tttgccatgg tgcccacaaa tatcatcgcg acatcaaacg gttcccagcg cttaggagta
     1261 aagccaaatg tattaaactg ttttggtaag agcgtctctg gattggtatt taccttatca
     1321 atccaggcat tcattccatc agcgtagcct tgcagaatgg acatatcctc tggggaaagg
     1381 gcatcaattt gcgcccggat agcatccggc cagtagttac gacggatatc tttatcaaat
     1441 ttcacaaaat ctttgccaag cacttccgcg acagtccctt gagtactgcg acgtgccatt
     1501 tccatctgaa aaaggcgatc ttgtgctact acatagccat agccataaaa taggtgccat
     1561 gtatcattgg cataa
//
LOCUS       DAJVUJ010000296         1574 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.299, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000296 DAJVUJ010000000
VERSION     DAJVUJ010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.299"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..90
                     /locus_tag="OQQ48_004235"
     CDS             <1..90
                     /locus_tag="OQQ48_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HCS1954760.1"
                     /translation="GIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            93..167
                     /gene="yoaJ"
                     /locus_tag="OQQ48_004236"
     CDS             93..167
                     /gene="yoaJ"
                     /locus_tag="OQQ48_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HCS1954761.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(226..474)
                     /locus_tag="OQQ48_004237"
     CDS             complement(226..474)
                     /locus_tag="OQQ48_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCS1954762.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(622..804)
                     /locus_tag="OQQ48_004238"
     CDS             complement(622..804)
                     /locus_tag="OQQ48_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HCS1954763.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(808..1168)
                     /gene="yeaR"
                     /locus_tag="OQQ48_004239"
                     /pseudo
     CDS             complement(808..1168)
                     /gene="yeaR"
                     /locus_tag="OQQ48_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein"
     gene            complement(1341..>1574)
                     /locus_tag="OQQ48_004240"
     CDS             complement(1341..>1574)
                     /locus_tag="OQQ48_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HCS1954764.1"
                     /translation="SFFVQFIDVNAPHTGISFFILATTLELVSFCYLSFLIISGAFVT
                     QYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
BASE COUNT          433 a          374 c          350 g          417 t
ORIGIN      
        1 ggtattattt ttccggttgt aatctttatt acagcggtcg tatttttagc atggtttttt
       61 attggcggct atgctgcccc gggggcataa agatgaaaaa aacaacgatt attatgatgg
      121 gtgtggcgat tattgtcgta ctcggcactg agctgggatg gtggtaacgt cacctctaaa
      181 aaatagcaaa ggctgcctgt gtgcagcctt tgtgcaattt aagcgttaac ttttaatctt
      241 cctgtagata aatagcacga caatcgcacc aataacggca accacgaagc tgccaaaatt
      301 gaagccatcg actttaccaa agccaaacag cgtgctgatc catccgccga ctacggcacc
      361 gactatcccc agcaggatag tcataaagaa tccaccacca tctttacctg gcatgatcca
      421 cttcgccaga ataccggcaa taagcccaaa aataatccat gacagaatgc ccattgtttc
      481 ctcacttatc tgttttgcat tagcgggtta gtcgctgata aaaagcatag cacaacatcg
      541 ggagggcaag atttgtgacg agcatcacgg aggatttttt tgcgatggcg cagaaattgc
      601 gccatcaacg atcagtgata attaccaacc acaaacatca tgttcgtttt ccgtgtcata
      661 agaacgcacg gtattcacca gatcttttat cacttcagcc gccacttctg gcaccagcaa
      721 agtcatcggc gtctctgttt cataatcgac agaaacgcca ttactgttat tggtgacggt
      781 cacggtatac gttgctttgc ccatgattca tttcccgtta tgaatgactt tccgttgttg
      841 cgcaccttcc atcaggactt caggagccac gaagaagtca atgttgacat aagtatcgtc
      901 agtcatggct tcaatgttgt gccacttttc tggagggaac accgcaaact gccccgcttc
      961 gataaggatc acctgatcag gctctgcact gtgttcatca gcgtagccga gatatttgac
     1021 cgccccatgc ataacggaaa ggcgtgggta acccccctgg cgcgttcctt tatcaagatg
     1081 acgttcgaat tattccggca ggcgcagttt gtttattcca gaaaggcgtt gagcgcgtat
     1141 gaatataatt ctgtgggatt tgcagcatcc ttttccctcc ttcggtgaat gcgctgaaaa
     1201 cggtttattc cagccgtttc agggtacgcc tgataatttg cattttaaat accatttatt
     1261 ggttactttt tagcaccata tcagagaaaa atcagggagg attacaagtg ggaagcccgc
     1321 gacaacgcgg gctgaatgta tcaggattgc agcgtcgcca gtcgggcggc gaaacccacg
     1381 aacatcaaac caatcagtga gttgccaact ttagccagtt tctttttggt acgtatgtac
     1441 tgcgtgacaa aagcaccaga aataatcagg aagctcaaat agcagaaact caccagttcc
     1501 agcgtcgtcg ccagaataaa gaatgaaatt cccgtatgtg gggcattaac atcgataaac
     1561 tgtacgaaaa acga
//
LOCUS       DAJVUJ010000297         1549 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.300, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000297 DAJVUJ010000000
VERSION     DAJVUJ010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.300"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(328..453)
                     /locus_tag="OQQ48_004241"
     CDS             complement(328..453)
                     /locus_tag="OQQ48_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001431667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HCS1954765.1"
                     /translation="MWLPVLFRFLVDSLATLGHRLAASGITSRMLSVIPVLFFWG"
     gene            956..1432
                     /locus_tag="OQQ48_004242"
     CDS             956..1432
                     /locus_tag="OQQ48_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS1954766.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVR
                     ELLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKN
                     ALVRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
BASE COUNT          402 a          382 c          403 g          362 t
ORIGIN      
        1 aaaaaaaacc gagccgccac ggttccggcc gcgccttgag cgctaccgga aaccgtctgg
       61 ggagaacccc agacccccat cgctgcaaca ccgacggtct acgaccacct accaaccgca
      121 tggctgcctg ttccgtgcaa atgagctttg cagggggctg gcgcggttcc tggcgacctg
      181 aaagcccctg gttaggggta tcggcgcgct tgacctcctg agagcctctg taagcgtttt
      241 tcgcgccttc catgccctgg cgggcatcta aaacccgttt cgtgcgtctg agagcttttg
      301 agcgcgtttg aggggctatc tggcgactca tccccaaaag aacaacaccg ggatcaccga
      361 caacatgcga gaggtgatcc cgctcgccgc aagtcgatga ccgagcgtag cgagcgaatc
      421 gacgaggaag cggaagagaa ctggaagcca cattgagcac ttacgcactg atgtggggtg
      481 tcggggtgaa gccctgacca agtggtaatc gtatcggcgt gcatgcgcgg ttatacgatt
      541 acacatcctg tcccgatttc tgaggcgttt taacggtgaa cggacggcaa aaacacgatg
      601 gagctgttga acgtgaaaac cacgaaatct ggcaaaaatc acggctggcg aggcttgcat
      661 aggagtaagc cagtatacac tccgctatcg ctacgtgact gggtcagggc ttcgccccga
      721 cacccccaaa gggcgttgcg ttgcacgcaa cacccttacc tagaatagat cttttacgcc
      781 gatttgtaag tggttgtttt tttagtgtgt tttttattta cctgtcgcat ccaggatgac
      841 acaacgttgt cgcatcctgg atgcgacaac acgcaacaag gtgttgcata gatgtgtcat
      901 gatggcttag atttgaacca acgttagtgc atgggatttt cagagggaaa aaatcatgtt
      961 tattgattca gaaaaacgac tgaaacaact ttcagatgag gcaaagaaaa acaccgagga
     1021 tctcgaagaa gcaaagaaaa attcaaggtt tacacaggta tccccaaaag gttgggaacg
     1081 tgttcgagag ctgctgaagg atagccaagg catatcagca ctgaagctgt actcattttt
     1141 agcggagcat atcgatccta cgtgtggcgc tgtcgttgcg gatcagcaat ttctagctga
     1201 aaaacttgga gttagcagaa gcacaattat tcggtggctc aattacttag aatcaaaaaa
     1261 tgcattagtt agaatccccg ttgctggtaa ggtttgtgcg tatgccctcg atccacatga
     1321 agtctggaag ggatacaaca ctacgaaaaa ccatgcagcg tttgtcacta aaacactggt
     1381 caacaaagac ggtgatattc agcgccgaat catggccatg ttttcaaatt gagctagcgg
     1441 caggcggaca atcaggggct acgtgttaac gttctgacca tgattgtcta tcctgcattg
     1501 ctcttttgcc gcctcaaaat cctttgcgtg tttttgctcc ccgttctcc
//
LOCUS       DAJVUJ010000298         1536 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.301, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000298 DAJVUJ010000000
VERSION     DAJVUJ010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.301"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(38..316)
                     /locus_tag="OQQ48_004243"
     CDS             complement(38..316)
                     /locus_tag="OQQ48_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HCS1954767.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSANVSAPAQST"
     gene            424..1134
                     /locus_tag="OQQ48_004244"
     CDS             424..1134
                     /locus_tag="OQQ48_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HCS1954768.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSDEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            complement(1137..>1536)
                     /gene="speG"
                     /locus_tag="OQQ48_004245"
     CDS             complement(1137..>1536)
                     /gene="speG"
                     /locus_tag="OQQ48_004245"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HCS1954769.1"
                     /translation="QSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKG
                     LATRAAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFTVEGELMHEFFINGQY
                     RNAIRMCIFQHQYLAEHKTPGQTLLKPTAQ"
BASE COUNT          421 a          336 c          376 g          403 t
ORIGIN      
        1 cgataatgta ttactgcggt tcggcaggcg cgccatccta ggtagactgc gcgggagcag
       61 agacgttagc gctggtggtg cgggtgtaga gaattttatg cgtatcatta gcacaatgcc
      121 cgacgacctg ggaatcaggc tgatcaacct ggtcattggg tacaatactt aatgtgaagc
      181 tgctttcggg tacgccatta ttgataatgc gctgtgatat atcgctctgt atgcgctcac
      241 aggatcccgg cgcggcgagt accgcgggtg aggcgagggc gagcagaagc gcggcacagc
      301 aggttgagag tttcatcata agctccttac gcgaagataa cttctttaag catagcattt
      361 aacgtgtaaa gtactgtatt tgctactatg attgagaatc atctctactc tctggtgact
      421 gttgtgaaat acaaattact accatgctta ctcgcgatat tcctcacagg atgtgaccgc
      481 acagaggtaa cactttcatt tacccctgag atggccagtt tctctgatga attcgatttt
      541 gatccgctgc gtggtccggt aaaagatttc actcagacat taatggatga gcaaggtgaa
      601 gtgacgaaac gtgtttctgg gactttgtcg gaagaaggct gttttgattc actcgaatta
      661 ctggatctgg aaaataatac cgtggtcgct ctggtactgg acgccaatta ttaccgtgat
      721 gccgagacgc tggagaagag agtacgttta cagggaaaat gccagctagc agaattacct
      781 tctgccgggg tgagttggga aaccgatgat aatggcttcg tgattaaagc cagcagcaaa
      841 caaatgcaga tggaatatcg ctatgatgat cagggttatc cactgggtaa aaccacgaaa
      901 agtaacgaca aaacattatc tgtcagcgcc acgccatcaa cggacccgat caaaaaatta
      961 gattacacag cggttacttt actgaataat caacgagttg gtaatgtaaa acagagctgt
     1021 gaatatgaca gtcacgccaa tccggtggac tgtcagctga tcattgttga tgaaggagta
     1081 aaacccgccg tcgaacgggt ttacaccatc aaaaatacga tcgattatta ttaatgctat
     1141 tgtgcggtcg gcttcaggag agtctgaccc ggtgttttgt gctctgccag atactgatgc
     1201 tggaatatac acatgcgaat ggcattacga tattgaccat taataaaaaa ctcatgcatc
     1261 aattcacctt caaccgtaaa gccaagcttg cggtaaatgt gaatcgcttt ttcattctct
     1321 ttatcgacga tcagatacag cttatagaga ttgagaacgg tgaagccata gtccattgct
     1381 aatttggcgg cacgggttgc cagacctttc ccctgatact ccggggagat aattatctga
     1441 aattctgccc ggcgatgtac atggttaatt tccaccagct ccaccagacc ggctttttcg
     1501 ccgtcacatt ccaccacaaa gcgtcgttcg ctctga
//
LOCUS       DAJVUJ010000299         1522 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.302, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000299 DAJVUJ010000000
VERSION     DAJVUJ010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.302"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(1..1386)
                     /locus_tag="OQQ48_004246"
                     /pseudo
     CDS             complement(1..1386)
                     /locus_tag="OQQ48_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
BASE COUNT          521 a          266 c          224 g          511 t
ORIGIN      
        1 agcgctggga gcttaccacc ataacaggcc catcatcgac agaggagaac aggataaata
       61 tattatcgtt tcgtgttaat ttattagtgt taaaggttct taacttttct tcagtttcta
      121 taaatatagg tgaaacatca tcactacgaa tccactcttt ataaagatac caggcatttg
      181 ctttgatcat atcatccgtt gatcgtaagg cctgatcgat aagttggata aaaaaattgt
      241 tatttttatc aatcagccct ttattcatat taaacctgtt aataaaatgg atcatctgtt
      301 gggtatcgaa atccaccttg ctgaatcgat gttgacgcat aaaatcatcg aacttgttat
      361 cttcaccaga atggatataa ctgcgtataa ttggatcttt tatataagga caatggctaa
      421 atgttttctt catattctca ataaagtttt tattaacaat attaagaatt tttaattgca
      481 taaccagttt tactgccaga acggatttca atatcattgc attatctggc aatgtatcaa
      541 tcatttttat aatgttattg atataaccat cgagatcttc attcccaaat atatctgaat
      601 aatcaatagt taattccaat tgttctgtaa tgcaatcttt aaaattccct gtcgacattc
      661 gcacatgatc aaacataaca gctgtcaaat tcgatttaat aaatgatatt tctttggcag
      721 acagaaactc tgctctggtg aagtgcgaag cgtaaagata acaattatcg aagtttgaat
      781 ttctaatctg acattcgcga aaattactat gaccaagttg actctgcgag aagtcaactg
      841 tatcaagaat atcttggcta aaatcaacac attcgccact cgttttgaga aaaatcacat
      901 tgtgtaaacg tcgacattta aatatgacat tatcaactgt tgccttagta aaattgcaat
      961 tctcaatttc tttacattcg aaagttacat gttgtagatt tgatgataaa aaagacacct
     1021 tagaaataac gtcaccttta aaatgacaat ttgttacatc aaaatcagac aggtcgatat
     1081 acccctgcga atctttatcc atcagaaaat tacaagcgca ttctattttt tcatttctgt
     1141 agaattttac acttatagta tcattactaa ttgagacttc acgtaacata tttattgcat
     1201 gattttttcg ttccgttgtt ttatatttaa aaatatatca ctgatataaa taaagttatc
     1261 tctgtttaaa cctgtcaccc cattgaaatt acgtagtaaa tcaagatcta ctatattatt
     1321 tccttgaacc acatcatgtt tcatcaaaga gttattgcca ccagaatttg tgatatgacc
     1381 tatcataaaa cctctgtaat acagaaataa aataacctgg aaaagtagcc tgagcgtcca
     1441 gacactaccg gcgaaaggaa aagaatctct gataaggcat tgagataatg gacattctta
     1501 gtgaagaaga agtgaagtac ta
//
LOCUS       DAJVUJ010000300         1522 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.303, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000300 DAJVUJ010000000
VERSION     DAJVUJ010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.303"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            68..808
                     /locus_tag="OQQ48_004247"
     CDS             68..808
                     /locus_tag="OQQ48_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCS1954770.1"
                     /translation="MFFNTKHTTALCFVTCMAFSSSSIADIVISGTRVIYKSDQKSVN
                     VRLENKGNNPLLVQSWLDTGDDNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTAST
                     SLPKDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPL
                     ALKWFWSGSEGKASLRVTNPTPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTG
                     LNGKASSAKVHFYAINDFGGTIEGNASL"
     gene            843..1522
                     /locus_tag="OQQ48_004248"
                     /pseudo
     CDS             843..1522
                     /locus_tag="OQQ48_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="similar to the fimbrial usher protein PapC;
                     frameshifted; incomplete; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein"
BASE COUNT          450 a          314 c          325 g          433 t
ORIGIN      
        1 gaatagtgtt aataatttct atttttatat tattccctgt tttaattaac tctgtcaggg
       61 atggtttatg ttttttaaca ccaaacatac aacagcttta tgcttcgtaa cctgtatggc
      121 ttttagttca tcgtctattg cggacattgt catttcgggt actcgcgtaa tatataaaag
      181 cgatcaaaaa agtgtcaacg tacgtctgga aaataaaggg aataacccgt tgcttgtcca
      241 gagttggtta gatactggcg atgacaacgc tgagcctggc agtattacag tcccttttac
      301 tgccacgccg ccagtatcgc gtattgatgc caaacgtggg caaacaatca aattaatgta
      361 cacagccagc acctcactgc ctaaagacag agagagcgtg ttctggttta acgtactgga
      421 agttccacca aaaccagatg cagaaaaggt cgcgaatcaa agtctgctgc aactggcatt
      481 tcgcacacgt ataaaacttt tctatcgccc ggatggattg aagggaaatc cctctgaagc
      541 cccgttagcc ctgaagtggt tctggtcagg ttcagaaggt aaggcgtcat tgcgagtgac
      601 caatccaacc ccttactacg tctcttttag cagtggtgat ttagaagcta gcggtaaacg
      661 ctatccgatt gatgtgaaaa tgattgcacc atttagtgat gaggtcatga aagtcactgg
      721 ccttaatggc aaagcaagtt ctgcaaaagt acatttttac gccattaatg attttggtgg
      781 caccattgaa ggtaatgcca gtctgtaatc aggcaaggat ataattccgc aggaagcata
      841 acgtgactat aaaaccaact aaaaattatc ataatcatct gacccgtatc gctacgtttt
      901 gcgcgctgct gtattgcaat agtgctttct gtgctgaact tgttgaatat gaccatacct
      961 tcctgatggg gcagaatgcc tcaaatattg atctcagccg gtatagtgaa ggtaaccctg
     1021 ctataccggg tatgtacgac gtcagtgttt atgtcaacga tcaacctatt attaaccaaa
     1081 gcattacatt tattgaaatt gaaggaaaaa aaacgcacaa gcctgtatca cacttaagaa
     1141 tttattgcag tttcatataa attctcccga tataaataac gaaaaagccg ttctgcttgc
     1201 cagggatgaa acactcggca attgccttaa tttgacggaa attattcctc aggcttctgt
     1261 tcgttatgac gttaacgagc aacgtctgga tatagacgtt cctcaggcat gggtaatgaa
     1321 aaattaccaa aactatgttg atccatcgtt atgggaaaat ggcattaatg cggccatgtt
     1381 gtcatacaac ctcaacggat atcatagtga aactcctggt cgaagaaatg acagcattta
     1441 tgctgctttt aacggtggga tgaatttagg tgcatggcga ctgcgtgcct caggcaacta
     1501 caactggatg accgattctg gc
//
LOCUS       DAJVUJ010000301         1466 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.304, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000301 DAJVUJ010000000
VERSION     DAJVUJ010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1466
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.304"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(<1..839)
                     /locus_tag="OQQ48_004249"
     CDS             complement(<1..839)
                     /locus_tag="OQQ48_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCS1954771.1"
                     /translation="MLNEGGILFKANHVPVLMYHHVSHCPGLVTLSPVTFRKQMKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLKEFNLKAHIFLIT
                     GFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFSSREEQCKHLRQDLLSGREYLKEMTGKCSKHLCWPEGYYNKDYIQVAEELG
                     FYYLYTTERRMNAPAKGTTRIGRISTKERESCAWLKRRLFYYTTPFFSSLLAFHKGPR
                     LPD"
     gene            complement(1203..1319)
                     /locus_tag="OQQ48_004250"
     CDS             complement(1203..1319)
                     /locus_tag="OQQ48_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004996477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCS1954772.1"
                     /translation="MPGIPPEIRWNNQKNALQLWNKEGGVVRREGDPLEMAQ"
BASE COUNT          434 a          337 c          261 g          434 t
ORIGIN      
        1 tcatccggta atcttggccc cttatgaaag gccagcaacg atgaaaaaaa cggtgttgta
       61 taataaaaaa gtcgtctttt cagccatgcg caactttccc gttctttggt gcttattctt
      121 cctatccgtg tagttccttt agcaggagca ttcattcttc tttctgttgt atataaataa
      181 tagaatccaa gttcttcagc gacctgaata taatccttat tataataacc ttccggccaa
      241 cacaaatgtt tactgcattt tcctgtcatt tctttcaaat attctctccc actcaacaag
      301 tcctgccgaa gatgtttaca ctgttcttct cgtgaactga actttttatc ccagcgtgta
      361 tgagtatgcg tatgaacatg aaattcgacc agaccagatt gcaacatttc gtttacttca
      421 gaccagcgaa gcataacatt atcagcattc cctgtcgcta tttgatgttc gcaatcgcga
      481 tgagaatatt cctttccagg agagtgacga accggaccat tcccaataaa accggtaatt
      541 aaaaaaatat gtgctttgag attaaactct tttaataaag gatatacctg aaaccagttg
      601 tcaaggtatc catcatcaaa tgtcaacata acgcttttcc gggggagttt tccgccccga
      661 taaaaaaact ccagttcatc tgatgacagt gttttccagt tattttcagc caaccatttc
      721 atttgcttcc ggaatgtcac gggagaaagt gtaaccagtc cggggcaatg actgacatga
      781 tgatacatta ataccggaac atgattggct ttgaacaaaa tgcccccttc gtttaacatt
      841 taaagtttct ggcacctagt aagcagagac aaaaggaaaa cccaataaag ctggtgcatt
      901 ttatttccag atactggatg aataacatag caaacccatg gcatactcga tatattttac
      961 taaattaaac atcaacaatt cataaacaat gatatattat catatctttt aaaaacgact
     1021 cactaagaac cttgaggtgg cacactgaat ttggctacct gaacagaggt gatatgttca
     1081 cctcagaaca acacaggtgc tccaatgaaa aaaaggaatt tcagcgcaga gtttatttac
     1141 ggggtgacgc tttcattaac ctgtacacga acttctattc cggcatcatg acaggcctgc
     1201 agctactgcg ccatttccag cggatcgccc tcccggcgta ccaccccacc ttctttattc
     1261 cataactgca gagcattttt ctgattgttc catctgattt cggggggtat tccgggcaag
     1321 gttgttcagt aaggcattcc ttcgtaaaat cagcatgttt tttgcacgac ggaaaccggc
     1381 agccatgaaa atctgtcttt atcatactct gaatcctgac acgatcccgg gttataaaaa
     1441 atttgcacag gctattgcca ccgata
//
LOCUS       DAJVUJ010000302         1450 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.305, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000302 DAJVUJ010000000
VERSION     DAJVUJ010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1450
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.305"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(<1..107)
                     /locus_tag="OQQ48_004251"
     CDS             complement(<1..107)
                     /locus_tag="OQQ48_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="chaperone-like protein that participates in the
                     polymerization of curlin (CsgA) subunits into curli
                     (extracellular fibers from Escherichia and Salmonella spp.
                     that are involved in the colonization of inert surfaces
                     and biofilm formation); part of the curli secretion and
                     assembly protein complex; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgE"
                     /protein_id="HCS1954773.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTV"
     gene            complement(112..762)
                     /gene="csgD"
                     /locus_tag="OQQ48_004252"
     CDS             complement(112..762)
                     /gene="csgD"
                     /locus_tag="OQQ48_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HCS1954774.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYSMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
BASE COUNT          457 a          271 c          285 g          437 t
ORIGIN      
        1 acagtatggt cagttagcaa tcccgggact tctacctcaa cggcgtgaag attccctgcg
       61 gcgaacagaa attctgccgc cacaatccag cgtaaataac gtttcatggc tttatcgcct
      121 gaggttatcg tttgcccagg aaaccgcttg tgtccggttt tttacggcta tcttcttgaa
      181 aagattataa agatgcgttt taaccgtatt ttcgctgatg aacaacgaac gagcgatctc
      241 gttattagac gcgccgatac gcagcttatt caggatctct ttttcccgat gagtaaggag
      301 ggctgattcc gtgctgttat aacgatagtt acctgaatgc gtaatcaagt agctggcaag
      361 cttttgcgta aagtagcatt cgccgcgcag gacgccttgc aacccattga caacacgttc
      421 ttgatcctcc atggaataaa aaacgccgtt gatatgaggc cagttttcaa tgtcgcggta
      481 cgggtaatct tcaggcgtat ttagcaacaa tattttgata ttgttgtttt tcctgctcaa
      541 agtatcctgc caataatgga taagcttttt atctgcttcc atcatatcca gaagaataat
      601 agagcctgaa gagatatcgt ccagagaacg ttgaatatta tgtaattttc ctgtaattgc
      661 cagcgattgt ttaaggtgct gcaagagagc tgtcgcctgc aaagaaggtt tagtgatcaa
      721 caataatgta tgaccatgaa tactatggac ttcattaaac atgatgaaac cccgcttttt
      781 ttattgatcg cacacctgac agctgcctct aaaatagaag caccagaagt actgacagat
      841 gttgcactgc tgtgtgtagt aataaatcag ccctaaatgg gtaaaatata aaactaatgg
      901 attacatctg atttcaatct agccattaca aaacttaaat caagtgttaa acatgtaact
      961 aaatgtaact tgttatatta aaatattaac cttaaggttt tattaagttt agaaatgata
     1021 gaaaagttgt acatttggtt tttattgcac aattttaaaa aatcatacaa atggtgataa
     1081 cttactaata atacatataa aaaatatttt ggtgtagtcc tttcgtcatg taaaacgttc
     1141 ttgttttttc tccgcacctc cgtggacaaa tttttactcc aaaaagacga ggtttgtcac
     1201 ggcttgtgcg caagacatat cgcagcaatc agcgacgggc aagaaaaatg actgtctggt
     1261 gatttttgat agcggaaaac ggagatttaa aagaaaacaa aatatttttt gcgtagataa
     1321 cagcgtattt acgtgggttt taatactttg gtatgaacaa aaaaagaaaa atacaacgcg
     1381 cgggtgagtt attaaaaata tttccgcaga catactttcc atcataacgc agcgttaaca
     1441 aaatacaggt
//
LOCUS       DAJVUJ010000303         1445 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.306, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000303 DAJVUJ010000000
VERSION     DAJVUJ010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.306"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..>1445
                     /locus_tag="OQQ48_004253"
     CDS             <1..>1445
                     /locus_tag="OQQ48_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCS1954775.1"
                     /translation="KLTTISELIKNIYCSLSVLIIGCASAYAVEFNKDLIEAEDRENV
                     NLSQFETDGQLPVGKYSLNAIINNKRTPIHLDLQWVLIDNQTAVCLTPEQLTLLGFTD
                     EIIEEAQQNLIDGCYPIEKEKQITTYLDKGKMQLSISAPQAWLKYKDANWTPPELWDH
                     GIAGAFLDYNLYASHYAPHQGDNSQNISSYGQAGVNLGAWRLRTDYQYDQSFNNGKSQ
                     ANNLDFPRIYLFRPIPAINAKLTIGQYDTESSIFDSFHFSGVSLKSDENMLPPDLRGY
                     APQITGVAQTNAKVTVSQNNRIIYQENVPPGPFAITNLFNTLQGQLDVKVEEEDGQVT
                     QWQVASNSIPYLTRKGQIRYTTAMGKPTSVGGDSLQQPFFWTGEFSWGWLNNVSLHGG
                     SVLTNRDYQSLAAGVGFNLNSLGSLSFDVTRSDTQLHNQDKETGYSYRANYSKRFEST
                     GSQLTFAGYRFSDKNFVTMNEYINDTNHYTN"
BASE COUNT          435 a          307 c          281 g          422 t
ORIGIN      
        1 aaaattaacc acaataagcg aattgattaa aaatatttat tgttcattat ccgttctcat
       61 cattggttgt gcgtcagctt atgccgttga attcaacaaa gatttaatcg aagccgaaga
      121 tcgtgaaaac gttaaccttt cccaatttga aactgatggc caattacccg taggtaaata
      181 ctcattaaac gctattatta ataataaaag gacgccaatc catctggacc tgcaatgggt
      241 attaattgat aaccaaactg cagtttgtct gactccagag caattaacat tattaggctt
      301 tactgatgag attattgaag aggctcagca aaacctgatc gatggttgtt accctatcga
      361 aaaagaaaaa caaattacaa cttatctcga taaagggaaa atgcaattat ccatatctgc
      421 acctcaggca tggttaaaat acaaagatgc aaactggacg cctcctgaac tttgggatca
      481 tggtattgct ggggcatttc ttgactacaa tttatatgcc tctcattatg caccacatca
      541 gggcgataat tcacaaaata taagttccta tgggcaggct ggggttaatc ttggggcctg
      601 gcgcctgcgt actgattacc agtacgatca gtcatttaac aatggcaaaa gccaggcgaa
      661 caacctggat tttccgcgta tttatttgtt tcgcccaatc ccagcaatta atgcaaaact
      721 aactataggt caatacgata ctgaatcctc tattttcgac tctttccatt tttctggcgt
      781 ttcgttgaaa agcgatgaga atatgttgcc gcctgatcta cgtggttacg caccgcaaat
      841 cacgggtgtt gcacaaacga atgcaaaggt cactgtctca cagaacaacc gtattattta
      901 tcaagaaaat gttcctcctg gcccatttgc tattactaat ttattcaata cattacaggg
      961 gcaactggac gtcaaggttg aagaagagga cgggcaagtc acgcaatggc aagttgcatc
     1021 taatagtatt ccttatctga cgcgtaaagg gcagattcgc tacaccactg ctatgggtaa
     1081 accgaccagc gttggtggtg attccttaca acaacccttc ttctggactg gtgaattctc
     1141 atggggttgg ctgaacaatg tatccctgca tggtggttca gttttaacaa accgtgatta
     1201 tcaatctctg gctgccggcg ttggctttaa ccttaactca ttgggttcat tatcttttga
     1261 tgtcacacga tctgatactc agttgcataa tcaggataaa gaaacgggtt atagctaccg
     1321 cgctaactat tcaaaacgtt ttgaatctac cggtagccag ctcactttcg ctggttaccg
     1381 tttctctgat aaaaactttg tgacaatgaa tgaatatatc aatgacacta accattacac
     1441 gaatt
//
LOCUS       DAJVUJ010000304         1405 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.307, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000304 DAJVUJ010000000
VERSION     DAJVUJ010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1405
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.307"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(1..483)
                     /locus_tag="OQQ48_004254"
     CDS             complement(1..483)
                     /locus_tag="OQQ48_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCS1954776.1"
                     /translation="MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNAS
                     RTFVICLEDTLRVVGYYSLATGSVTHAELGRSLRHNMPNPVPVVLLGRLAVDVCIQGH
                     GFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV
                     "
     gene            complement(474..776)
                     /locus_tag="OQQ48_004255"
     CDS             complement(474..776)
                     /locus_tag="OQQ48_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCS1954777.1"
                     /translation="MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRT
                     DFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLEAPVQNAEGRERLMAVKPEWK"
     gene            1048..1320
                     /locus_tag="OQQ48_004256"
     CDS             1048..1320
                     /locus_tag="OQQ48_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1954778.1"
                     /translation="MSHNLEHQKVHTRMVKEVLKAVARANNHPYKSVFADFITGHPSC
                     TVCFWETFHKMYPDSPYEYVTFCHTCRRFDLYETEAEMKADDPKWW"
BASE COUNT          405 a          358 c          304 g          338 t
ORIGIN      
        1 tcagacttta taaaacaagg tattaggtgc cacaactgac tgaacaaaac caaaacgctc
       61 ataaaatgct ctggcgtcat catcaattgc atgtaccata acagccttaa tgccaacctg
      121 atcagccaga ttgacaaccc ggtgaatggc atcacttagt agccatttac cgaaaccatg
      181 cccctggata caaacatcaa cagccagacg ccctaacaga acaacaggga caggattggg
      241 catgttatgc cgcaaactgc gaccaagctc ggcatgagtg acggaaccag tagctaatga
      301 gtagtatccc acaacacgta aagtatcctc caagcaaata acgaacgtgc gagaagcatt
      361 gagcatctgg tttttcatag cccgaccacg taaccagtta cttagcacct catttccaca
      421 gtcaaatggc tgaagtatat gctcatccgt caacaatgct ggcgcggtga catttatttc
      481 cattcaggct ttaccgccat cagacgttca cgcccttctg cattctgcac aggagcttcc
      541 agttgcgtaa taaacgaatc ataacgttcg ttatccagca caaataaacg ttgatccagg
      601 ataatgtttt tagcttcctg gtacgccagt tccatgatga aatcggtacg gttcttattg
      661 accaagctcg cagcataatc aataacggct cgttcctcat cagttgcccg gatgttaatc
      721 tgatgttctc gcggtgtttt acggacgctt gcagctgtag acataaaaac ctccattggt
      781 aacaatttgc tatgcaatta atgttatcgc aaatggtaac agaacgctat acagaatcac
      841 ttacgggaaa agcgatcccc tgataaaaac aatggcagag acctgaacat tgtgacgatg
      901 atctgtgtcg gcgcacaaat catgtctgat tacggccgtt taatgtgatt ttgataatga
      961 aatgcctgaa ccacggcagc ctggcggctg caaatcgggg tgtggcagaa tatgagcttt
     1021 cagagtaacc atccagaatg agttcccatg tcacacaacc tggagcacca gaaagtccac
     1081 accagaatgg tgaaagaggt tctgaaagcc gtcgccagag ccaataatca cccttataag
     1141 tccgtatttg cagactttat caccggtcat ccctcctgca cggtctgttt ctgggaaacc
     1201 ttccacaaaa tgtacccgga ttcaccgtac gaatacgtca cgttctgcca tacctgccgt
     1261 cgttttgatt tatacgaaac agaagcggag atgaaggcgg atgacccgaa gtggtggtga
     1321 ttttcagccc cgaccgctga aacgcctgtt cacagccaac cagtgctgga cgtccttcct
     1381 ggatgcgggc ggtctgcgcg atatc
//
LOCUS       DAJVUJ010000305         1365 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.308, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000305 DAJVUJ010000000
VERSION     DAJVUJ010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1365
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.308"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(72..137)
                     /locus_tag="OQQ48_004257"
                     /pseudo
     CDS             complement(72..137)
                     /locus_tag="OQQ48_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease"
     gene            370..474
                     /locus_tag="OQQ48_004258"
                     /pseudo
     CDS             370..474
                     /locus_tag="OQQ48_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            814..1071
                     /locus_tag="OQQ48_004259"
     CDS             814..1071
                     /locus_tag="OQQ48_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm development YmgB/AriR family protein"
                     /protein_id="HCS1954779.1"
                     /translation="MHSDYYNMVFGEKLANILYEANSQFFYERNVIEEAVNALFCERE
                     IINNKNIIKKLMFFLSDVNHTKKDVVQSALNIIIDITSGDI"
BASE COUNT          442 a          256 c          260 g          407 t
ORIGIN      
        1 gctgcatgac aaggtcatcg ggcattatct gaacataaaa cactatcagt aagttggagt
       61 cattacctcc tcccgaactc aaagctatca tacagataat tactggttaa ccctatatag
      121 ggcattttaa agtgttgtga tcttacccag caatagtgga cacgcggcta agtgagtaaa
      181 ctctcagtca gaggtgactc acaagacaaa acagtttcaa ccagtaaaaa aacgtaaaca
      241 ccattcgcat gaatttcgta gttaagatct gaagcttgca gaatgcatcg gtgttactgc
      301 cggagtccgt gaacttagtc tgcatgaatc accacagctt tacatctgtc gcagtaaaca
      361 gcaaaatcag caaacttact taattatatc gagtgcgatt acaatcgctg gcgatggtac
      421 agttggtgtg gcggtctcag tccggaacaa tttgaaaatc agaatctcac ttaaaacggc
      481 atccatttta caaaagatgg ccgtctgtgt atttgtaatg aataccttct acatgtttag
      541 agtgcataat ccacgacaag gcgatatcct aaaaattagc tagcaggcaa ctttgatggc
      601 atagataatg tttttagtaa aaaagagttg actgaatata atacagcctc ttcctacata
      661 agaaggaatg tgctcacaaa aaaaaaccat tagtctgctt cctgttctta caataatcag
      721 tattatgtat tttatttata tagcaaaata tgcttattac acctgtcata attaggcatg
      781 gtaaaattat tgactgaacc ctgggggaga aagatgcaca gtgattatta taatatggtt
      841 tttggtgaaa aattggccaa tattttatat gaagctaact ctcaattttt ttacgagaga
      901 aatgtcatag aagaggcagt caacgcttta ttctgtgagc gtgaaataat aaacaacaag
      961 aacataataa agaagctgat gttctttttg tcagatgtta atcacaccaa gaaagatgtt
     1021 gttcagagcg cacttaatat tatcattgac attacatcag gtgatatttg agattttaca
     1081 taaaagataa tctctcaagg agtcttatct gaaaacgaaa tatatagtcc ctctcgtttt
     1141 gtaatatcga ttggctttgt tgaatcggtt acgtaccccg gagctggtca tattttcagg
     1201 taatacacac gctttcagac atttcggctt tcgtcatttt ggtatcatgg gcatagcttc
     1261 tgctacctga gcatcataat cacgcagctt cagtgaagca gtgaccacct gtttaacccg
     1321 gtgtatcgtt atgatcttat ccagcaataa tggacacgcg gctaa
//
LOCUS       DAJVUJ010000306         1334 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.309, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000306 DAJVUJ010000000
VERSION     DAJVUJ010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.309"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..1245
                     /locus_tag="OQQ48_004260"
     CDS             <1..1245
                     /locus_tag="OQQ48_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCS1954780.1"
                     /translation="SNGKLRPLGIPALRDRIVQRAMLMAMEPIWESDFHTLSYGFRPE
                     RSVHHAIRTVKLQLTDCGETRGRWVIEGDLSSYFDTVHHRLLMKAVRRRISDARFMTL
                     LWKTIKAGHIDVGLFRAASEGVPQGGVISPLLSNIMLNEFDQYLHERYLSGKARKDRW
                     YWNNSIQRGRSTAVRENWQWKPAVAYCRYADDFVLIVKGTKAQAEAIREECRGVLEGS
                     LKLRLNMDKTKITHVNDGFIFLGHRIIRKRSRYGEMRVISTIPQEKARNFAASLTALL
                     SGNYSESKVDMAEQLNRKLKGWAMFYQFVDFKAKVFSYIDRVVFWKLAHWLARKYRTG
                     IASLMRWWCKSPKPGQSKTWVLFGKTNHGKLSGEILYRLVGQGKKLFRWRLPEGNPYL
                     RTETRNTYTSRFTEVAMAFASI"
BASE COUNT          340 a          324 c          388 g          282 t
ORIGIN      
        1 agcaacggca aactgcgacc actgggtatc cccgcgttgc gggatcgtat tgttcagcgg
       61 gccatgctga tggcgatgga gccgatatgg gagagtgatt ttcatacgct ctcatatggc
      121 ttccggcctg agcgcagtgt ccaccacgcg atccgcacgg tgaaattaca gctcacagac
      181 tgcggtgaaa cccggggacg ctgggtgatt gaaggcgacc tgtccagtta cttcgacacc
      241 gtacatcatc gactgctgat gaaggctgta cgccgcagga tcagtgacgc acgtttcatg
      301 actctgctgt ggaaaaccat caaggcggga catatcgatg tcggtctctt tcgggcggcc
      361 agtgaaggtg tgccacaggg cggtgttata tcgccgctat tatcgaacat catgctgaat
      421 gagttcgatc aatacctgca tgagcgctac ctgagcggga aagccagaaa agatcggtgg
      481 tactggaata acagtatcca acggggccga agtacggcgg tcagagaaaa ctggcagtgg
      541 aaacccgcgg tggcgtactg ccgctatgcc gatgattttg tcctcatcgt caaaggcacc
      601 aaagcacagg cggaagccat cagggaggag tgtcggggtg tgctcgaagg cagtctgaaa
      661 ctcaggctga acatggataa gactaaaatc acccatgtta atgacggctt tatctttctg
      721 gggcacagga tcattcgcaa acgcagtcgt tatggcgaga tgcgagtgat ctcaacgatc
      781 ccgcaggaga aagccagaaa cttcgccgca tcgctgacag cactgttatc aggcaactac
      841 agtgaaagca aagtcgatat ggctgaacaa ctcaaccgaa aactgaaagg ctgggccatg
      901 ttctatcagt tcgttgattt taaggccaaa gtcttcagtt atatcgaccg tgtcgtgttc
      961 tggaagctgg ctcactggct ggcccgcaaa taccgtacag gtatcgcttc cctgatgagg
     1021 tggtggtgta aatcaccgaa accgggtcag agcaaaacgt gggttttatt tggtaaaacc
     1081 aatcacggca agctcagcgg cgaaatactg taccggttgg tggggcaagg caagaagctg
     1141 ttccgctggc ggctacccga aggtaatccc tatctgagga cggagaccag aaacacgtat
     1201 acatcgcgct ttacagaagt ggcaatggcg ttcgccagca tttaaatgga gagccggatg
     1261 cgctgaaagg tgcacgtccg gttcggggag gagaggcagg gaaatagtcc gactacgccc
     1321 tgcctcttac tcta
//
LOCUS       DAJVUJ010000307         1325 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.310, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000307 DAJVUJ010000000
VERSION     DAJVUJ010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1325
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.310"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(<1..175)
                     /locus_tag="OQQ48_004261"
     CDS             complement(<1..175)
                     /locus_tag="OQQ48_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory CII family protein"
                     /protein_id="HCS1954781.1"
                     /translation="MFDYQVSKHPHFDEACRAFALRHNLVQLAERAGMNVQILRNKLN
                     PAQPHLLTAPEIWL"
     gene            complement(208..429)
                     /locus_tag="OQQ48_004262"
     CDS             complement(208..429)
                     /locus_tag="OQQ48_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HCS1954782.1"
                     /translation="MRPNISITLTTPHVTIERYSELTGLSIDTINDMLADGRLIRHRL
                     RKDKKREKVMINIAAMTVDALSECNLNLN"
     gene            555..1124
                     /locus_tag="OQQ48_004263"
     CDS             555..1124
                     /locus_tag="OQQ48_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein CI"
                     /protein_id="HCS1954783.1"
                     /translation="MNLEKGGRGAIERMVEAYGFKTRQALCDHLGISKSTLATRYMRD
                     SFPAEWVIQCALETGTSLNWLTTGHGSKQTSGNTNTMEVAKYVLSDGALREDGFYIFD
                     KGFLPSTFKKPFVITDNNSEFICDKEFDDIRDGKWVISIDGEVTIRDITRLPGGRIFV
                     EGGNRAFECKIEDIEIIGKIISLTVKYVR"
     gene            1140..>1325
                     /locus_tag="OQQ48_004264"
     CDS             1140..>1325
                     /locus_tag="OQQ48_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1954784.1"
                     /translation="MLGKVFFVVLSCSLLLSPLTTYAKNYPCSGKKGGVSHCTSDGKF
                     VCNDGTISKSKKICTKNS"
BASE COUNT          394 a          239 c          299 g          393 t
ORIGIN      
        1 gcagccagat ttctggtgcg gttaataaat gaggctgagc tgggttcagc ttgttccgca
       61 gaatctgcac attcatgcct gcacgttctg ccagttgcac cagattgtgg cgcagtgcaa
      121 atgcacgaca ggcttcatca aaatgtggat gtttggaaac ttggtaatca aacatggtca
      181 atgcctctga tgtatttcag aatcgaacta attaaggttt agattgcatt ctgaaagcgc
      241 atcaacggtc attgctgcta tgttgatcat cactttttcg cgttttttat ctttgcgcag
      301 acggtgacgg ataaggcgtc catcagccaa catgtcattg atggtatcga tggatagccc
      361 tgtcagctcg ctatagcgtt caatagtcac atgaggcgtg gtaagagtga ttgaaatgtt
      421 aggtctcatg atgcaacatt cctcgtttaa tgatgattaa tcaggacgaa tacggatcgt
      481 ttgtattttg tgaacaccat aaacatatga ttgcacagtg aaatcgtcaa gataaaagtt
      541 cacttggagt gaccatgaat ttggagaaag gcggacgagg cgccatagag cgcatggtag
      601 aagcttatgg attcaagact cgacaggcgt tgtgcgatca tttaggaatc tctaaaagta
      661 cactcgccac acgctacatg cgtgactcat tcccagcaga atgggtaatc cagtgcgccc
      721 ttgaaacggg cacctcgctt aattggctca caaccggaca tggttcaaag caaacttcag
      781 gtaatacaaa tactatggaa gttgctaaat atgtattatc tgatggtgcc ttgcgtgaag
      841 acggttttta tatttttgat aagggatttc taccctctac gtttaaaaaa ccttttgtca
      901 tcacagataa caattctgaa tttatttgtg ataaagaatt tgatgacata cgtgatggta
      961 aatgggtaat aagtattgat ggcgaagtaa cgatccgtga cattactcgt ttacccggtg
     1021 gaagaatctt cgtcgagggt ggaaacagag ccttcgaatg taagatagaa gacattgaaa
     1081 taattggtaa aattataagt ttaacagtta aatatgttag ataataccgg gaggaaacta
     1141 tgcttggtaa agtatttttt gtggttttat catgctcttt gttattaagc ccactaacta
     1201 cctatgctaa aaattatcct tgttctggga aaaagggagg tgtctctcac tgtacctccg
     1261 atggaaaatt tgtttgcaat gatggaacta ttagtaaatc caaaaaaatc tgtactaaaa
     1321 actca
//
LOCUS       DAJVUJ010000308         1320 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.311, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000308 DAJVUJ010000000
VERSION     DAJVUJ010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1320
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.311"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(70..492)
                     /locus_tag="OQQ48_004265"
     CDS             complement(70..492)
                     /locus_tag="OQQ48_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1954785.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELPLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSSDDSVVVH"
     gene            complement(476..703)
                     /locus_tag="OQQ48_004266"
     CDS             complement(476..703)
                     /locus_tag="OQQ48_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCS1954786.1"
                     /translation="MLKIDAIAFFGSKTKLANAAGVRLASIAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRPGKVIHENQA"
     gene            780..1187
                     /locus_tag="OQQ48_004267"
     CDS             780..1187
                     /locus_tag="OQQ48_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCS1954787.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPAILTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
BASE COUNT          368 a          347 c          269 g          336 t
ORIGIN      
        1 ccgaaccttc ctccggatat aatctgtgat tccccaatca acagaaccag aggaggttcg
       61 gcataaacat caatgcacaa cgacagaatc gtcggacgac ccaccaccgc tgaaatgtgc
      121 tttccggtaa acggcctgaa ctgcgtcatc atgcgcatca attgccgtac tcaacgcttc
      181 ctgcgccgcc agtaatgcac ggcgttccag ggtatcgaag atacagagtc ggtgacgcag
      241 ctcacgcgga agaattgcca gaaccgcagg gatcagtttc tgaattttct ccctttgcgc
      301 tttcgtttca cctttcaacc aacggtgata gatattctgc tgattgttcc agtccttgcc
      361 tggtaccaga ggcaattcgc cgcccccctg ccgcagatat tcttcagtaa ttgcgttagc
      421 gacccacgcc tgcccttttt cggctgccag ggctaacagc actgattcga tgtgctcatg
      481 cctgattttc atgaatcacc ttcccaggtc gttttgcctt acgatattcg tcataaactt
      541 tggggtcgta ctgaagttcc ccgccagatg cctcttgcag gcgcatcgcg cggccttcag
      601 gaaccagttc cccccatgca gcaatgcttg ccagcctaac tcctgcggca ttggcaagct
      661 ttgttttgct gccaaaaaac gctatagcat caattttcaa catatcgagc tccttagatt
      721 ttcctaagga aactagatcg tagagaaacc taagtcaaga aagattagaa ttccctaata
      781 tgaaaaacga aaccttcggt gctcgcctct tacataggcg taaaaaatta aaactgtctc
      841 aggccgcatt aggtaagctg gtcaaagtgg ctcacgtaac aatttctcaa tgggaaagag
      901 atgaaacaca gccggcgggg aaaagattat tcgcactgag ccaagcgctt cagtgctcac
      961 cgacttggct tctttttggg gatgaagata aacaaccagg cgaaccgatc ccggataatc
     1021 agccagccat tctgacagaa gatcaaaaag agttacttca actgttcgac gcactgcctg
     1081 agtcagagca aaaggccctg ttgtcagaga tgcgtgctcg agttgaaaat ttcaacaaac
     1141 tttttgaaga actactcaaa gctcgcaaaa gaagcgcaaa caaataatcc ccttttttct
     1201 ccacaccctg taataaaaag cacaaacttt caaacacttg tgttttttac atcaaaaaaa
     1261 cttaggtttt tatacataaa atcttgactc cacgtcttag gttattctaa atttcattca
//
LOCUS       DAJVUJ010000309         1318 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.312, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000309 DAJVUJ010000000
VERSION     DAJVUJ010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1318
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.312"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(25..>1318)
                     /gene="pdeF"
                     /locus_tag="OQQ48_004268"
     CDS             complement(25..>1318)
                     /gene="pdeF"
                     /locus_tag="OQQ48_004268"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HCS1954788.1"
                     /translation="SYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDPVVHLPNVRAL
                     NRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLEPGEDVYQLSG
                     NDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRSPVNHIYLLLG
                     ELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNHFFLMAQPITG
                     MRGDVYHEILLRMKGENDELISPDSFLPVAHEFGLSSSIDMWVIEHTLQFMAENRAKM
                     PAHRFAINLSPTSVCQARFPFEVSQLLAKYQIEAWQLIFEVTESNALTNVKQAQITLQ
                     HLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLDYQIVASICHL
                     ARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKP"
BASE COUNT          360 a          336 c          299 g          323 t
ORIGIN      
        1 ttcattcaac gtatcaatta acggctacgg cttaccaata agataaccct gcatataatc
       61 gatccccaaa gagagcaccg cctcgcggat ctcttcgttt tcaacgtact ctgccactac
      121 ccgcattttc ttcattcgcg ccaggtggca aatcgatgcc actatctgat aatctagact
      181 atttgacaca atattgcgga taaaactgcc gtcaatttta agcagatcgg cattcacatt
      241 tttaagccgt gcatagctgg cgtagccggt gccgaaatca tcaatcgcaa tctggcagcc
      301 taattcctga agatgctgca aggtgatttg cgcctgctta acattggtca gagcattact
      361 ttcggtgact tcaaaaataa gttgccacgc ttcaatctga tatttagcca gcaactgact
      421 gacttcaaaa ggaaaacgag cctgacatac cgaggttgga gacagattaa tagcaaaacg
      481 gtgagcgggc atcttcgctc tgttttcagc cataaattgc agcgtatgct caatgaccca
      541 catgtcgata ctcgacgata aaccaaattc gtgcgcgacc ggcaagaagc tatcggggct
      601 gatcagttca tcattctcac ctttcatgcg cagaagaatt tcatggtaaa catcaccacg
      661 cataccggta atcggctggg ccatcaggaa aaaatggttg tgttccagcg cctgctgtag
      721 tcgattcatc atcgcgactt tatctttcaa ttcgcgttgc aaatacattg ccccgcgacg
      781 ctgcatattt tccggggcgt tggtcacgat ggaaagttcg gcgaccgtat ttagctctcc
      841 cagcagcagg tagatatgat tcactggcga gcgcacatag cagtaactga cgccaatctg
      901 cggttgcatc ggcatgccat cccaaaagaa acgaaattgc ttgagatggc tatccagtgc
      961 ggtaatgcgc tcctggtgcg attctgtatt cagtcgcagc gcgagatcgt tacccgaaag
     1021 ctgataaaca tcttcacccg gttccagcaa gggtgacagc cagtgagaaa gtttttgctt
     1081 gtattgaatc cgcagcatga tgccatagtt cttaaccagc atttccatgc cagggatgcg
     1141 taaataacaa agcgcagacc agggggcatc acgcaacgcg cgattcaggg cgcgaacatt
     1201 tggcagatga accaccgggt ccacatacgc aagccgctga atgcgtctga ctaccgctct
     1261 ctgacgggtt gccagtacag ccatataatt gacaataaaa gagaatacca gataactg
//
LOCUS       DAJVUJ010000310         1316 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.313, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000310 DAJVUJ010000000
VERSION     DAJVUJ010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1316
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.313"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..978
                     /locus_tag="OQQ48_004269"
     CDS             <1..978
                     /locus_tag="OQQ48_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCS1954789.1"
                     /translation="CEHLPLYRQSEIFARQGVELSRALLSNLVDACCQLMTPLNDALY
                     RYVMNSRKVHTDDTPVKVLAPGRKKAKTGYIWTYVRDDRNAGSPEPPAVWFAYSPDHQ
                     GKHPEQHLSPFRGILQADAFNGYDRLFSAEREGGALTEAGCWAHARRKVHDVYISTKS
                     ATAEEALKLIGELYAIEHEIRGLPVSERLAVRQMQSKPLLTSLYKLMQEKEHTLSKKC
                     RLRDAFRYIRKHWVALCNFSDDGLAEADNNAAERALRAVCLGKKNFMFFGSDHGGERG
                     ALLYGLIGTCRLNGIDPEAYLRYILSVLPEWPSNRVDELLPWNVALTNK"
     gene            1204..1316
                     /locus_tag="OQQ48_004270"
                     /pseudo
     CDS             1204..1316
                     /locus_tag="OQQ48_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
BASE COUNT          329 a          321 c          383 g          283 t
ORIGIN      
        1 tgcgaacacc tgccactgta tcgtcagagt gaaatttttg cccgtcaggg tgtcgaactg
       61 agccgtgcat tactctccaa cttggttgac gcgtgctgcc agttaatgac gccgctgaat
      121 gatgctctgt accgttatgt aatgaacagc cgcaaagttc acactgatga cacaccagta
      181 aaagtgctgg caccgggcag gaagaaggcg aaaacaggat atatctggac gtatgtccgg
      241 gatgacagga atgccggttc gccagagcct ccggcggtct ggttcgccta ctcaccggac
      301 catcagggta aacatccgga gcagcacctt agtcccttcc ggggtatcct gcaggcagat
      361 gcgtttaatg gttacgatcg gctgttcagt gccgaacgag aaggcggcgc gttgacggaa
      421 gcaggatgct gggctcatgc gcggcgcaaa gtccacgatg tatatatcag taccaaaagc
      481 gcgacagcgg aagaagccct gaaactaatc ggtgagctgt acgccatcga gcacgaaata
      541 cgcgggttgc cggtgtctga acgcctggcg gtcaggcaaa tgcagagtaa accgctactg
      601 acttccctgt ataagctgat gcaggagaaa gaacacacgt tatcgaaaaa atgccgtctg
      661 agagatgcgt tccggtatat caggaagcac tgggttgcgt tgtgcaactt cagtgatgat
      721 ggtctggctg aggcggataa taatgccgcg gaaagagcgc ttcgtgcagt ctgtctcgga
      781 aagaaaaact ttatgttctt cggcagcgat cacggtggag agcgtggtgc gctactgtac
      841 gggctgatcg gcacctgccg actgaacggt atcgatccgg aagcgtatct gcgctatatc
      901 ctgagcgtac tgccggaatg gccttccaac cgtgttgacg aactcctgcc atggaacgta
      961 gcactcacca ataaataagc gtcaatacgg tgctccgttg acgcttacag tggatgcagt
     1021 ctaaatacag actttcgaac cggaaggctc agcgaaagct ggcgctggta aggaaacagt
     1081 atccgaagct atttgcccac tggtatttac tacgtgcatc gaatgagtga tcaagagccg
     1141 tatgacgcga gagtgtcacg tacggttccg tgagcagccg gagagtgaaa ctctctccga
     1201 cggggttccg atagcggtgg cgaacatgcg gcggtgttgt actcgctgat cggcacatgc
     1261 cgtctgaaca atgtggagcc agaaaaatgg ctgcgttacg tcattgagca tatcca
//
LOCUS       DAJVUJ010000311         1285 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.314, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000311 DAJVUJ010000000
VERSION     DAJVUJ010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1285
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.314"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            368..1156
                     /gene="virF"
                     /locus_tag="OQQ48_004271"
     CDS             368..1156
                     /gene="virF"
                     /locus_tag="OQQ48_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family invasion system transcriptional
                     regulator VirF"
                     /protein_id="HCS1954790.1"
                     /translation="MMDMGHKNKIDIKVRLHNYIILYAKRCSMTVSSGNETLTIDEGQ
                     IAFIERNIQINVSIKKSDSINPFEIISLDRNLLLSIIRIMEPIYSFQHSYSEEKRGLN
                     KKIFLLSEEEVSIDLFKSIKEMPFGKRKIYSLACLLSAVSDEEALYTSISIASSLSFS
                     DQIRKIVEKNIEKRWRLSDISNNLNLSEIAVRKRLESEKLTFQQILLDIRMHHAAKLL
                     LNSQSYINDVSRLIGISSPSYFIRKFNEYYGITPKKFYLYHKKF"
     gene            complement(1098..>1285)
                     /locus_tag="OQQ48_004272"
     CDS             complement(1098..>1285)
                     /locus_tag="OQQ48_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCS1954791.1"
                     /translation="APQKQLQSLRSLSFIERNENIVLLGPSGVGKTHLAIAMGYEAFK
                     IFYDISKISLELYHNIH"
BASE COUNT          442 a          202 c          222 g          419 t
ORIGIN      
        1 aatatcaaca ccaaccggat ttgtggagcc tccagtctga aggggcttta tgcgttccgt
       61 ataggatatt atgatgctgg agttttgcga agctccactc ctttccgaac atttacacag
      121 atattgccta agaaaagtga aaccataata tggttatagt ccctttcagt gcaaatactt
      181 agcttgttgc acagagaaat agaagctgca taagctcttt cttcaaaaaa tgtaaataaa
      241 gttaaatata ggaaaaatta cttaatctat cttaataacg gaaagatttt gtatacaatc
      301 actgttacag aaatttctta gttactctgt aaacactaaa tatagtttgg tatattctgt
      361 tgaatttatg atggatatgg gacataaaaa caaaatagat ataaaggttc gcttgcataa
      421 ctatattatt ttatatgcaa aaaggtgttc aatgacggtt agctcaggca atgaaacttt
      481 gactatcgat gaagggcaaa ttgcttttat agagcgaaat atacaaataa acgtctccat
      541 aaaaaaatct gatagcatta atccatttga gattataagc cttgacagaa atttattatt
      601 aagcattatt agaataatgg aaccaattta ttcatttcaa cactcctatt ctgaggagaa
      661 aagggggtta aacaaaaaaa tattcctcct ctctgaggag gaggtttcta tcgatttgtt
      721 caaatctata aaagagatgc cttttggcaa aagaaagatc tatagtttag cttgcctttt
      781 atcagctgtt tctgatgagg aagctttata tacttcgata tcgatagctt cttctcttag
      841 tttttctgat cagataagga agattgttga aaaaaacatc gagaagagat ggcgtctttc
      901 tgatatttca aataacttga atttatcaga aatagctgtt agaaaacgat tggagagtga
      961 aaaattaaca tttcaacaaa tccttcttga tattcgcatg catcatgcag caaagctttt
     1021 attgaatagt caaagctata ttaatgatgt atcaagactt atcggaatat caagcccatc
     1081 ttattttata aggaaattta atgaatatta tggtataact ccaaagaaat tttacttata
     1141 tcataaaaaa ttttaaatgc ttcatagccc atcgctattg ccagatgggt tttccccaca
     1201 cctgatggcc ctagtaatac gatattttca ttacgttcta tgaagctgag tgagcgtaac
     1261 gactggagtt gcttctgcgg tgctc
//
LOCUS       DAJVUJ010000312         1273 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.315, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000312 DAJVUJ010000000
VERSION     DAJVUJ010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1273
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.315"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(57..896)
                     /locus_tag="OQQ48_004273"
     CDS             complement(57..896)
                     /locus_tag="OQQ48_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCS1954792.1"
                     /translation="MLNVAIENQNGWNYSAPAPHKTGAGISTPKVTRAHNRAEAVFLC
                     VMHSHIQIMVRRAGQPQGWPGSRVTGSANPVRLTTHEISTSAGELINLSLEDVIMATT
                     LSHPDVTIENGRAVTTSISIAEFFGKQHHHVVQKIESFECSELFLTSNFSRVKFEHRG
                     NSYNAYQITKNGFVFLVMGFTGKKAATFKEAYIAEFDRMEAELRQNNTPPADKMIPGD
                     GRTLVVHFDKFGNVEFTETVPDGALVCTLETFRFYLEKQGWTLVNRGAIKNMTVEQLL
                     SLK"
BASE COUNT          330 a          312 c          296 g          335 t
ORIGIN      
        1 tgagggcagg tgtaaaacaa taatcaagca tggggttcat atccatagct ccatttttat
       61 tttaaggaaa gcaactgctc tacggtcatg tttttaattg cgccccggtt tacaagagtc
      121 cagccctgtt tttccagata aaaccggaaa gtctccaggg tacagaccag tgcgccatca
      181 ggaacggttt cggtgaattc gacattgccg aatttgtcga agtgaacaac cagagtgcga
      241 ccatcacccg gaatcatctt gtcagcaggt ggggtgttat tctggcgcag ttcggcctcc
      301 atgcggtcga actcagcaat gtaggcttcc ttgaatgtgg cggctttttt gcccgtgaag
      361 cccatcacca ggaaaacgaa gccgtttttg gtgatttggt aggcgttata agaattgccg
      421 cggtgttcga atttaacccg cgaaaagttg ctggttaaaa atagttcaga acattcgaag
      481 gattctattt tttgaacaac atggtgatgc tgcttgccaa aaaactcagc aatcgaaata
      541 gaagtagtga cggcgcgacc attttcgatg gttacgtcag ggtgagaaag ggtagtagcc
      601 ataatgacat cctcaagtga taagttaatt aactcaccag cagaggtgct aatctcatgg
      661 gtggtgagac gcacagggtt agcactaccg gtcactcgag aacccggcca gccttgcggc
      721 tgccccgcac gcctcaccat aatttgaatg tggctgtgca ttacgcataa aaaaaccgct
      781 tcagcgcggt tatgcgctcg agtaactttc ggggtgctaa tcccggcacc cgttttatga
      841 ggtgcaggtg cactataatt ccacccgttc tggttttcaa tagctacatt caacattttc
      901 tctttccttt catcaccgaa gtgaattttg tgatgcggtg cctagtgcct ccaggtgacg
      961 ttaaccagtt aacaattaca gtcggctttc ccacccaaac caataaggac taacatgact
     1021 tttaactgtg ccgcgtgcgc ttagccgcat tcaccgcatc acaaaattca ctttaaaaag
     1081 ggcggacatc agccagcaat taaaccgatg ccgccaactg gtacttcaca cagcaatgtc
     1141 gttatttaca accggaagcg cactcccacc atttaaattt cacagacaag accgactctt
     1201 tatgaatacc ggaaatgcgc cttcgtgttg tgcccggttt tatttcacca cctccgggct
     1261 ttggtggcct cgg
//
LOCUS       DAJVUJ010000313         1228 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.317, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000313 DAJVUJ010000000
VERSION     DAJVUJ010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1228
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.317"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(<1..784)
                     /gene="ipaH4.5"
                     /locus_tag="OQQ48_004274"
     CDS             complement(<1..784)
                     /gene="ipaH4.5"
                     /locus_tag="OQQ48_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH4.5"
                     /protein_id="HCS1954793.1"
                     /translation="MKPINNHSFFRSLCGLSCISRLSVEEQCTRDYHRIWDDWAREGT
                     TTENRIQAVRLLKICLDTREPVLNLSLLKLRSLPPLPLHIRELNISNNELISLPENSP
                     LLTELHVNGNNLNILPTLPSQLIKLNISFNRNLSCLPSLPPYLQSLSARFNSLETLPE
                     LPSTLTILRIEGNRLTVLPELPHRLQELFVSGNRLQELPEFPQRLKYLKVGENQLRRL
                     SRLPQELLTLDVSNNLLTSLPENIITLPICTNVNISGNPLSTR"
BASE COUNT          349 a          221 c          267 g          391 t
ORIGIN      
        1 cgcgagtcga caatggattc cctgaaatgt taacattcgt acaaatgggc aatgtgatta
       61 tattttcggg taatgaagtt agtaggttat tggaaacatc cagtgtcaat agttcttgcg
      121 gtaatctgga taatctgcgt agttgatttt cacctacctt caaatatttt aagcgctgag
      181 gaaattctgg tagttcctgt agtctgttgc cggaaacaaa gagttcttgt agtctatgag
      241 gcaattcagg caagacagta aggcgattac cttcaatacg taatattgtt agcgttgatg
      301 gaagctctgg taacgtctcc agactattaa aacgtgccga gagtgattgt aaatatggtg
      361 gtaatgatgg cagacatgac aaatttcgat tgaatgaaat attaagctta atcagttgag
      421 atggaagtgt cgggagtata ttcaagttgt taccatttac atgaagttct gtcaaaagcg
      481 gagaattttc aggtagggag attaactcat tgttggaaat attaagttca cgtatatgca
      541 aagggagtgg tggtaaagaa cgtagtttca gtaagcttaa attgagaaca ggctcccggg
      601 tatccagaca tattttcaat aatcgaaccg cctggatgcg attttctgtt gttgttcctt
      661 ccctagccca gtcatcccag atgcggtggt aatctctggt acactgttct tctaccgata
      721 aacgagatat acatgataag ccacaaaggg aacgaaaaaa agaatgattg ttgatcggtt
      781 tcatttatta acccctcact attcatatct tggtctatta atgcaaataa aacgagaaag
      841 tctttaaaaa aacaatccta gagctttatc ggcgttttca ttccagctgg aggaatatgt
      901 tcctgtcttt ccagtaaccg tcgattgtta tatcagtcta cccatgttaa tgtgaccagt
      961 tctactttca tccagtttgg attgatcttt atcccctata ttttcaaaca atttttatca
     1021 tttaactcat tactgtctcg atattcccat agcaaacgat ctggttggtt agcccctcaa
     1081 aaaagtcagt tgcacgccgt aaatccctgc cggtattgag cgaggattta cgggttgttg
     1141 ttaaggtccg taatcgggtc acagtttttc cggagtcaat ccggtctgcg gtttatgctt
     1201 atgcgacgtg attatgaatg gtgcagtc
//
LOCUS       DAJVUJ010000314         1201 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.318, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000314 DAJVUJ010000000
VERSION     DAJVUJ010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1201
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.318"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            59..1069
                     /locus_tag="OQQ48_004275"
     CDS             59..1069
                     /locus_tag="OQQ48_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HCS1954794.1"
                     /translation="MDKITQVLFSDIGKVTTQYVEVPHQELKPHEVRIAPVFYGICGS
                     DLHVLKGGHPFAKPPVVPGHEIAARVTEVGSDVKNVQPGDHVVVDPIMACMECRACKA
                     GRFNLCEPPQVASFRAPGFARSQHIVPARNCHVAPASLPLKVLAFAEPAACARHCVNR
                     MPKASLESVLVIGAGTIGLSIVQALRIMGAGKITVIEPDAAKRALALKLGAAEVWAPG
                     ELAADVRFTGAIDVVAAQATLNDACTRVYAGGTVVCMGVPSGPREIPLPMMQRFERDL
                     LNSGMYIPEDFDAVIEWLADGRFDTSELVTDLFAIEDAAAAFERAQQNDSIKVMLQFA
                     PE"
BASE COUNT          284 a          294 c          324 g          299 t
ORIGIN      
        1 tcgatgaagg tatcgcgcaa cgctaatccc ctatggatat cgattctgga gaataacaat
       61 ggataaaatt acccaggttt tattttcaga tattgggaaa gtcaccactc aatatgttga
      121 agtaccacac caggaactta aaccgcacga agtgcggatt gcgcctgtgt tctatgggat
      181 atgcggttcg gatctgcatg ttctgaaagg cggtcatccg tttgccaaac cacctgtcgt
      241 ccccggtcat gaaattgcag cgcgcgttac ggaagttggc agcgacgtta aaaatgtaca
      301 gccgggcgat catgttgtgg tcgatcccat catggcttgc atggaatgcc gagcctgcaa
      361 agcaggacgt tttaatcttt gtgaaccacc gcaggttgct agttttcgcg caccgggctt
      421 tgctcgctca caacacattg ttcctgcgcg taattgccat gtcgcaccag cctctttacc
      481 gctaaaagtg ttggcctttg ccgaaccggc ggcttgtgcc cgtcactgcg ttaaccgaat
      541 gccgaaagct tctctggaaa gcgtactggt aattggtgcc ggaacgatag gcttatccat
      601 cgtgcaggca ctgcgcatta tgggggcagg taagattacc gtgattgaac ctgacgctgc
      661 caaacgcgcg ctggcgttaa agctgggcgc agcagaagtt tgggcaccag gtgagctggc
      721 cgcagatgtg cgatttacgg gggcgattga tgtcgttgca gcgcaggcca cgcttaacga
      781 tgcatgtacc cgtgtatatg ccggaggcac cgtcgtgtgc atgggcgtac caagtgggcc
      841 gcgtgaaata ccattaccga tgatgcaacg tttcgagcgt gacttgctca actctggcat
      901 gtacatccct gaagatttcg atgctgttat cgaatggctg gcggatgggc ggtttgatac
      961 cagtgaactg gttaccgatt tatttgccat tgaggatgca gcggcggcat ttgaacgcgc
     1021 gcagcaaaat gactccataa aggtcatgct gcaatttgcg ccggaatgat tttctaaatc
     1081 tctgtctggc gctgcgtcac gtcagcgcca ccctacaaca attaaaaagg acactgcgat
     1141 gagtcaaatc accagtcctg caacttattc aattagtcgg ccacaggacg taattgatat
     1201 t
//
LOCUS       DAJVUJ010000315         1171 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.319, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000315 DAJVUJ010000000
VERSION     DAJVUJ010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1171
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.319"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..359
                     /locus_tag="OQQ48_004276"
     CDS             <1..359
                     /locus_tag="OQQ48_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1954795.1"
                     /translation="VVAELGDDSGWHFYSQRNTDGSITFAVNGQVIPLNYGNFDARYK
                     HRTEGVQDVRYGYEMYYNPGSNTVSWTFRSPSGHGLSGISISDTGRNSADNVNGVYYR
                     PLQKLINGIWYNVASI"
     gene            362..907
                     /locus_tag="OQQ48_004277"
     CDS             362..907
                     /locus_tag="OQQ48_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCS1954796.1"
                     /translation="MLHLKNITAGNPKTAEQYQLTKQYDVTWLFSEDGKNWYEEQKNF
                     ASDTIKMVYTGDGRVVWVGKDVTGIEPRNASVIEVPDITANRRITAPGYWFYRNDEFV
                     FDYRLKAEDERDALLAQVSARTGEWEEDLLLGLISDEDREKLKAYRIYAKSLQAMDFS
                     VITDKTSYNAIEWPASPEISS"
     gene            931..>1171
                     /locus_tag="OQQ48_004278"
     CDS             931..>1171
                     /locus_tag="OQQ48_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCS1954797.1"
                     /translation="MSVVISGALTDGAGIPMSGYHIILKSRVNTPEVVMHTVADVMTG
                     NDGEYCFHARTGKYGVYLKPGWHNEYNVGDIAVYED"
BASE COUNT          326 a          254 c          307 g          284 t
ORIGIN      
        1 ctgttgttgc agagcttggt gatgattccg gctggcattt ctacagccag agaaataccg
       61 atggtagtat cacattcgct gtaaacggac aggtaattcc gttaaattac ggaaacttcg
      121 atgcccgcta taaacatcga actgaggggg tacaggatgt gcggtatggt tatgaaatgt
      181 attacaaccc tggcagcaac accgtttcat ggacgtttcg ctcaccttcg ggacacgggt
      241 tgtcagggat atcgatatcg gataccggcc gtaactcagc ggataacgtc aacggtgtgt
      301 attaccgacc gctgcaaaaa ctgattaatg gcatctggta taacgtagcg agtatttaac
      361 aatgttgcat ttaaaaaata ttacggcagg taatccgaaa accgcggaac aatatcagct
      421 gacaaaacag tatgatgtca cctggctttt ttcggaagac ggcaaaaact ggtatgaaga
      481 acagaagaac tttgccagtg acaccataaa aatggtttac accggagacg ggcgcgtggt
      541 gtgggtcggt aaggatgtga caggcattga accccgtaac gccagtgtta ttgaagttcc
      601 tgatattacc gccaaccgcc gcattaccgc gccgggttac tggttttacc gcaatgatga
      661 atttgtcttt gactacagac tcaaagcgga agatgagcgt gatgcccttc tggctcaggt
      721 cagtgcccgg acaggggaat gggaagaaga cctgctgctg gggctaatca gcgacgaaga
      781 ccgggaaaaa ctgaaagcgt accgcattta cgcgaaatcg ctgcaggcga tggatttcag
      841 tgtcatcact gataaaacct catacaacgc cattgaatgg cccgcctctc cggaaatttc
      901 ttcctgattt aatttatcgc gagaaaaact atgtctgtag tgatatcagg tgcgctgact
      961 gatggcgcag gtatccccat gtccggatac catattattc tgaaatcccg ggtaaacacc
     1021 ccggaagtgg tgatgcacac tgttgctgat gtgatgacag gaaacgatgg tgaatactgt
     1081 ttccatgcgc ggactggaaa atatggtgtg tatctgaaac cgggctggca caacgagtac
     1141 aacgttggcg acattgctgt atatgaggac t
//
LOCUS       DAJVUJ010000316         1163 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.320, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000316 DAJVUJ010000000
VERSION     DAJVUJ010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1163
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.320"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..209
                     /locus_tag="OQQ48_004279"
     CDS             <1..209
                     /locus_tag="OQQ48_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCS1954798.1"
                     /translation="CYDNAPMESFWGTLKNESLSHYRFKSRDEAISVIREYIEIFYNR
                     QRRHSRLGNISPAAFREKYHQMAA"
     gene            complement(251..1163)
                     /locus_tag="OQQ48_004280"
                     /pseudo
     CDS             complement(251..1163)
                     /locus_tag="OQQ48_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT          357 a          273 c          271 g          262 t
ORIGIN      
        1 actgttacga caacgctccg atggaaagct tctggggaac gctgaaaaat gaaagcctga
       61 gccactatcg ttttaagagc cgggatgaag ccatctcagt aatacgggaa tacattgaga
      121 ttttctacaa tcgtcagcgt cgtcactctc gtctggggaa tatctccccg gcagccttca
      181 gggaaaaata tcatcagatg gctgcttaaa aaaagaacaa atggtagtgt ccgctattgc
      241 cagtacacct cataccacag agaataccaa ggaagatggc atttagaatt tgcaattcca
      301 gctcaaccgc cggagtcgtc gccatgagta cgctggcgta aaaacacata ccactcacga
      361 tagcaatgag cattaatagt cgcttgccaa tacgtttcat gtaatagcct gcgattaaca
      421 tcatcggaat ttccagaccg gcagcgatac caatcatttc tccagccagt ttatcggtta
      481 gatgcagttc atcaataata aatagcggca tatttatcat gtagagatta ttcgccgccc
      541 acatcattga acagaccaca aaaagtagcc gcgtatcccg cttcctatgg gtggagggta
      601 aaatttctac gggttgggta acgacaggaa tatttctttg tattgatggc aaaaacaacc
      661 agactatcag cccgcaaaca acaaatgcga tggcagcggt gagatacatc actttaaaac
      721 taaaccccat tgccagttca taagcgagcg gtggcccgat aacccaggca agcgagatct
      781 gagcacgtaa aaatgtactg aacatgaccg tctcacggcc tgttctgtcg gcgtgttcac
      841 gggcgagggc gaacatttgc gggtttgcgg tagaagcaaa actactcaga agtacgcccg
      901 tagagaggag aatgaagtag ttgcgattcc acgcaaaaag cgtgcaggcc agcactccaa
      961 ataagcaaca tagcagaatc agtaatttac ggtcgccttg tttatcggag tgccttgcca
     1021 gaaattgact gaccagaatt cccataatag cgctaccggt gaagaaaaaa cctaccatta
     1081 taggacgggc tttcagttca tctgcgagga atatacttag ggtaggagtc tgaagagcgc
     1141 ccgcaatacc cgtcagaaag gcg
//
LOCUS       DAJVUJ010000317         1155 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.321, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000317 DAJVUJ010000000
VERSION     DAJVUJ010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.321"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(1..263)
                     /locus_tag="OQQ48_004281"
                     /pseudo
     CDS             complement(1..263)
                     /locus_tag="OQQ48_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(378..1016)
                     /gene="ospI"
                     /locus_tag="OQQ48_004282"
     CDS             complement(378..1016)
                     /gene="ospI"
                     /locus_tag="OQQ48_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspI"
                     /protein_id="HCS1954799.1"
                     /translation="MINGVSLQGTAGYEAHTEEGNVNVKKLLESLNSKSLGDMDKDSE
                     LAATLQKMINPSGGDGNCSGCALHACMAMLGYGVREAPVPNEISEYMTGFFHRHLEQI
                     DSEGIVSHPNETYSKFRERIAENILQNTSKGSVVMISIEQATHWIAGFNDGEKIMFLD
                     VQTGKGFNLYDPVEKSPDAFVDENSSVQVIHVSDQEFDHYANSSSWKSKRLC"
BASE COUNT          338 a          284 c          176 g          357 t
ORIGIN      
        1 agcaagcgct gtaactcttt tatttgtttt atagcggcag ccagttctga ggcaggaacg
       61 acatcctctc cggcagcaac agcagtgaga cttccttcca gatattattt tttccactta
      121 agcaacaggc tgggctgaat accatgcaaa cgcgccacat gggacaccgt catgccaggc
      181 tccattgtct gctgaataat ggcaattttt tcctgaggag ttttacgttt acgaagctcc
      241 agccccagca aagttccagt catctcaaaa ttagaactag tgttagacat atatccaagc
      301 ctatctctta ttttaagata catccactgt ctggcgtttc aaggggccgg ctcaaatcta
      361 tgtacaggct ctcagcatca gcaaagcctc ttactcttcc aactactact gttagcatag
      421 tgatcaaatt cctgatcaga tacatgaatt acctgaaccg aactattttc atctacaaat
      481 gcatctggcg atttttctac aggatcataa agattaaatc cctttcctgt ttgaacatcc
      541 aaaaacataa ttttttcacc atcgttaaat ccagctatcc aatgagtggc ctgttcaata
      601 gaaatcatta ctacacttcc ttttgaagtg ttttgtaaaa tattttctgc aatacgctca
      661 cgaaatttac tataagtttc attaggatgt gacactatgc cttcactatc aatttgctct
      721 aaatgcctat gaaaaaatcc ggtcatatac tctgatattt cattaggtac gggagcctct
      781 cttactccat accctaacat agccatacaa gcatgaagcg cacaccctga acagttacca
      841 tccccaccag acggatttat cattttctgt aaagtcgctg ccaattcaga atctttatcc
      901 atatcaccaa gacttttaga gtttaaagat tccagcaatt ttttaacatt tacatttccc
      961 tcctctgtat gtgcctcata tccagcagtt ccctgtaacg acaccccatt aatcatattg
     1021 tatcctgaca gtatatatac tcactatatt ttcttcataa caaatatccc tctgatatga
     1081 caacaaaaaa cggagccgga ttcctgtttt tgtgaactgc agctattcga gtctaaaaag
     1141 tgatccactt accac
//
LOCUS       DAJVUJ010000318         1153 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.322, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000318 DAJVUJ010000000
VERSION     DAJVUJ010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1153
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.322"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            12..1028
                     /locus_tag="OQQ48_004283"
     CDS             12..1028
                     /locus_tag="OQQ48_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element IS5 family transposase"
                     /protein_id="HCS1954800.1"
                     /translation="MFVIWSHGTGFIMSHQLTFADSEFSSKRRQTRKEIFLSRMEQIL
                     PWQNMVEVIEPFYPKAGNGRRPYPLETMLRIHCMQHWYNLSDGAMEDALYEIASMRLF
                     ARLSLDSALPDRTTIMNFRHLLEQHQLARQLFKTINRWLAEAGVMMTQGTLVDATIIE
                     APSSTKNKEQQRDPEMHQTKKGNQWHFGMKAHIGVDAKSGLTHSLVTTAANEHDLNQL
                     GNLLHGEEQFVSADAGYQGAPQREELAEVDVDWLIAERPGKVRTLKQHPRKSKTAINI
                     EYMKASIRAKVEHPFRIIKRQFGFVKARYKGLLKNDNQLAMLFTLANLFRADQMIRQW
                     ERSH"
BASE COUNT          321 a          304 c          306 g          222 t
ORIGIN      
        1 gatatgagat catgtttgtc atctggagcc atggaacagg gttcatcatg agtcatcaac
       61 ttaccttcgc cgacagtgaa ttcagcagta agcgccgtca gaccagaaaa gagattttct
      121 tgtcccgcat ggagcagatt ctgccatggc aaaacatggt ggaagtcatc gagccgtttt
      181 accccaaggc tggtaatggc cggcgacctt atccgctgga aaccatgcta cgcattcact
      241 gcatgcagca ttggtacaac ctgagcgatg gcgcgatgga agatgctctg tacgaaatcg
      301 cctccatgcg tctgtttgcc cggttatccc tggatagcgc cttgcctgac cgcaccacca
      361 tcatgaattt ccgccacctg ctggagcagc atcaactggc ccgccaattg ttcaagacca
      421 tcaatcgctg gctggccgaa gcaggcgtca tgatgactca aggcaccttg gtcgatgcca
      481 ccatcattga ggcacccagc tcgaccaaga acaaagagca gcaacgcgat ccggagatgc
      541 atcagaccaa gaaaggcaat cagtggcact ttggcatgaa ggcccacatt ggtgtcgatg
      601 ccaagagtgg cctgacccac agcctagtca ccaccgcggc caacgagcat gacctcaatc
      661 agctgggtaa tctgctgcat ggagaggagc aatttgtctc agccgatgcc ggctaccaag
      721 gggcgccaca gcgcgaggag ctggccgagg tggatgtgga ctggctgatc gccgagcgcc
      781 ccggcaaggt aagaaccttg aaacagcatc cacgcaagag caaaacggcc atcaacatcg
      841 aatacatgaa agccagcatc cgggccaagg tggagcaccc atttcgcatc atcaagcgac
      901 agttcggctt cgtgaaagcc agatacaagg ggttgctgaa aaacgataac caactggcga
      961 tgttattcac gctggccaac ctgtttcggg cggaccaaat gatacgtcag tgggagagat
     1021 ctcactaaaa actggggata acgccttaaa tggcgaagaa acggtctaaa taggctgatt
     1081 caaggcattt acgggagaaa aaatcggctc aaacatgaag aaatgaaatg actgagtcag
     1141 ccgagaagaa ttt
//
LOCUS       DAJVUJ010000319         1138 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.323, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000319 DAJVUJ010000000
VERSION     DAJVUJ010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1138
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.323"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(<1..93)
                     /locus_tag="OQQ48_004284"
     CDS             complement(<1..93)
                     /locus_tag="OQQ48_004284"
                     /inference="COORDINATES: protein motif:HMM:NF024418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ESPR domain-containing protein"
                     /protein_id="HCS1954801.1"
                     /translation="MNKIYSLKYSHITGGLVAVSELTRKVSVGTS"
     gene            complement(454..>1138)
                     /locus_tag="OQQ48_004285"
     CDS             complement(454..>1138)
                     /locus_tag="OQQ48_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003557916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HCS1954802.1"
                     /translation="NSTGQEVDKSRLTSQAFARAALITDVGIALATELETAGQKWSLG
                     FPPKFQRVDLFNYNVLVRNYDSSAFKGDRYHNTKNGINADIGASTNLDDNWTLGLVAQ
                     NLIPRSIETKEVNGITETFRIRPQVTAGVSWHNAMFTTAFDVDLTPASGFTSDSNRQF
                     AAIGAEFNAWKWAQLRAGYRQNLAGNDGSAFTAGVGISPFDVVHLDVAGLIGTDNTYG
                     AVAQFQFTF"
BASE COUNT          267 a          263 c          298 g          310 t
ORIGIN      
        1 tgatgtaccg acactaactt tccgggtcag ttcagaaaca gcaactaatc cacctgtaat
       61 atgactatat ttcagtgaat aaattttatt catacgtgaa ctccgatatt tctgagttgt
      121 ggttatggga tagcatttaa ttacaacggg gaactatcag tcatggtacg taactgataa
      181 gggtataatg ataatcactg tcagttatat ttgtatgaca gatgtaacaa aatgaaaaaa
      241 aagataagca caagtgattg attggtcaga tattgatcac ctggtgtttg tattttgacc
      301 tttgcgatca aaatttgatc tttgttgttt ccggtcaatc ctttacaggt tttctgtatt
      361 atccggggga gttaagtctg tcgatgtccg acagactttt gatgatggaa acaacatgca
      421 gcgataatgt gcatgctgtt ttgcaggtgg aactcagaag gtgaactgga actgtgcgac
      481 cgcaccgtaa gtgttgtccg tgccgatcag gcctgcaaca tcaaggtgaa ccacatcaaa
      541 cggtgagatc cccaccccgg ccgtgaatgc actgccgtcg ttaccggcca gattctgacg
      601 gtaaccggcg cgcagctgtg cccatttcca ggcattaaat tctgcgccaa tggcggcaaa
      661 ctgacggttg ctgtcggagg tgaaaccgct ggccggggtc agatccacat caaatgcggt
      721 ggtgaacatc gcgttgtgcc aggagacacc ggccgtcacc tgcgggcgga tcctgaaggt
      781 ttccgtgata ccgttcactt cttttgtctc aatactacgg gggatcaggt tctgtgcgac
      841 cagtcccagc gtccagttgt catccaggtt cgtactggca ccgatgtcgg cgttgatgcc
      901 atttttcgtg ttgtggtagc ggtcgccttt gaaggcgctg ctgtcataat ttctgaccag
      961 cacgttgtag ttgaacaggt cgacgcgctg gaatttgggg gggaagccca gagaccattt
     1021 ctgcccggcc gtttccagtt ccgtagccag agcaataccc acatcagtga tcagtgccgc
     1081 gcgggcaaag gcctgtgatg tcaggcggct tttgtccacc tcctgcccgg tgctgttt
//
LOCUS       DAJVUJ010000320         1119 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.324, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000320 DAJVUJ010000000
VERSION     DAJVUJ010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1119
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.324"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            281..>1119
                     /locus_tag="OQQ48_004286"
     CDS             281..>1119
                     /locus_tag="OQQ48_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCS1954803.1"
                     /translation="MLNGGGILFKANHVPVLMYHHVSHCPGLVTLSPETFRKQMKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLNEFNLKAHIFLIT
                     SFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKLTSREEQCKHLRQDLLSGREYLKKMTGKCSKHLCWPEGYYNKDYIQIAEELG
                     FHYLYTTERRMNAPAKGTTRIGRISTKERENCVWLKRRLFYYTTPFFSSLLALHKGPR
                     LPD"
BASE COUNT          339 a          195 c          234 g          351 t
ORIGIN      
        1 ttctggtcaa caaccagttg agcggattcg cgtttaaact ctgcgctgaa atttcttttt
       61 tcattatggc acctgtgttg ttctgaggtg agcatatcac ctctgttcag gtagctaaat
      121 ttagtgtgcc acctcaaggt tcttagtgag ccgtttttaa aagatatgat aatatattat
      181 tgtttataaa ttgttgatgt ttgatttagt agtatataaa gttcaccaac tttattaggc
      241 tttcctttta tctctactta ctgggtgcca gaaactttaa attttaaacg gagggggcat
      301 tttgtttaaa gcaaatcatg ttccggtatt aatgtatcat catgtcagcc attgcccagg
      361 actggttaca ctttctcccg agacattccg gaaacaaatg aaatggttgg ctgaaaacaa
      421 ctggaaaaca ctgtcatcag atgaactgga gtttttttat cggggcggaa aacttccccg
      481 taaaagcgtt atgttgacat ttgatgatgg ataccttgac aactggtttc aggtatatcc
      541 tttattaaac gagtttaatc tcaaggcaca tattttttta attaccagtt ttattggaaa
      601 tggtccggtt cgtcactctc ctggaaagga atattctcat cgcgattgcg aacatcaaat
      661 agcaaccggg aatgctgata atgttatgct tcgctggtct gaagtgaacg aaatgttgca
      721 atctggtctg gtcgaatttc atgttcatac acatactcat acacgttggg ataaaaagct
      781 cacttcacga gaagaacagt gtaaacatct tcggcaggac ttattgagcg ggagagaata
      841 tttaaaaaaa atgacaggaa aatgcagtaa acatttgtgt tggccggaag gttattataa
      901 caaggattat attcagatcg ctgaagaact tggattccat tatttatata caacagaaag
      961 aagaatgaat gctcctgcta aaggaactac acggatagga agaataagca ccaaggaacg
     1021 ggaaaattgc gtatggctga aaagacgact tttttattat acaacaccgt ttttttcatc
     1081 gttgctggcc cttcataagg ggccaagatt accggatga
//
LOCUS       DAJVUJ010000321         1099 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.325, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000321 DAJVUJ010000000
VERSION     DAJVUJ010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.325"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(<1..778)
                     /gene="virK"
                     /locus_tag="OQQ48_004287"
     CDS             complement(<1..778)
                     /gene="virK"
                     /locus_tag="OQQ48_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HCS1954804.1"
                     /translation="MFSVSNLSFIGFLKRIVFSSDSLPGKWEHRKFRFMYILRCAINP
                     VASIRYYYELRSLPCIEDILAIHPTLPARIHRPYLHKGGRAWTRGQYILEHYRFVQNL
                     PEKYSKFLFPQKSVSLVQFIGKDGENFDIQCSSSGFDREGELMLSLFYNKTVITRLTF
                     SVILTQNGHIAFIGGLQGAPKNTGPDVIRCATRACYGIFPKRIIFEAFCALMKACNIS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTC"
     gene            complement(783..>1099)
                     /gene="capU"
                     /locus_tag="OQQ48_004288"
     CDS             complement(783..>1099)
                     /gene="capU"
                     /locus_tag="OQQ48_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HCS1954805.1"
                     /translation="YRVASLVVLPSENESFGMVLAEASAFSVPVLASQIGGIPDVIQN
                     NQTGTLLPAGNKHAWMCALNDFFNDPGRFYQMARQAKQDIEERFDINKTALKILTLAK
                     HK"
BASE COUNT          338 a          253 c          212 g          296 t
ORIGIN      
        1 cacaggtttt tcctgctaca gactcccaga aatcactata gaccgcaaca aaggttttgc
       61 gtttctgata ccagtatctc aattgccgga aaacatgact gtgctcactg actgccagac
      121 actcagagat attgcaagct ttcatcaacg cacaaaatgc ttcaaaaata atccgttttg
      181 ggaaaatccc ataacaggcc cgggttgcgc accgaataac atcaggtccg gtatttttag
      241 gggctccctg taatccgcca ataaaagcaa tgtgtccatt ttgagtcaga atgactgaaa
      301 atgttaaccg ggtaataact gttttattgt aaaacaatga caacatcaac tctccttccc
      361 ggtcaaaacc actggaagaa cactggatat caaaattctc tccatctttc ccgatgaatt
      421 ggactaatga taccgatttt tgtggaaaca aaaatttaga atatttttct ggcaaatttt
      481 gcacaaagcg ataatgctca agaatatatt gtccacgggt ccaggcgcgc ccccctttat
      541 gcaaataagg gcgatgaatt cttgctggca acgtggggtg tattgccaga atatcctcaa
      601 tgcacggcaa ggaacgcagt tcgtaataat atcgaatact ggcaaccgga tttatagcac
      661 atcgcaaaat gtacatgaac cggaattttc tgtgttccca cttccccggg agtgaatctg
      721 aggaaaaaac aatcctttta aggaaaccga taaatgataa gttacttaca gaaaacatat
      781 tgttacttgt gcttcgctaa tgtgagtatt tttaacgcag ttttattaat atcaaaccgc
      841 tcttctatat cctgttttgc ctggcgagcc atctgataaa aacgcccagg gtcattaaaa
      901 aaatcattca gggcgcacat ccatgcgtgc ttattacctg ctggtaacaa tgtcccggtc
      961 tggttgttct gaataacatc agggattcca ccaatctgac tggccagtac aggcacagaa
     1021 aatgccgatg cttctgccag taccatacca aacgattcgt tttctgaagg cagaaccacc
     1081 agactggcaa cccgatata
//
LOCUS       DAJVUJ010000322         1092 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.326, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000322 DAJVUJ010000000
VERSION     DAJVUJ010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1092
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.326"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(198..464)
                     /locus_tag="OQQ48_004289"
     CDS             complement(198..464)
                     /locus_tag="OQQ48_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1954806.1"
                     /translation="MLTQTIFPCLPQKQENIILEVSNPVLLSSTVTTDGYTVFNKKAA
                     IYELQIPATNRTKTLKFTATEMQWLTKINEVGIVEKQSQRHSNI"
     gene            complement(646..1044)
                     /locus_tag="OQQ48_004290"
                     /pseudo
     CDS             complement(646..1044)
                     /locus_tag="OQQ48_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612066.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
BASE COUNT          281 a          224 c          226 g          361 t
ORIGIN      
        1 cgtccctgca ctcgaataat ggtgaagaaa gcatgatgaa tatatcacga taacgttcag
       61 tatgtatcga ggatagatgg agttactcct ctctgaaata gaattactgc tctacttttt
      121 ctgtttccag aaattattag taacaaaccc tgatatattc ttttgaaaat ggagcttaaa
      181 cgagtctgaa attaacttca gatattagaa tgccgttggc tttgtttttc aacaattcct
      241 acttcattta tctttgtcaa ccattgcatt tctgtagcag tgaatttcaa cgttttcgtc
      301 cggttggtag caggtatttg taattcgtat atagctgctt ttttattgaa aacagtatat
      361 ccatctgtag taacagtaga agataataat acaggatttg acacttcaag gatgatattt
      421 tcttgttttt gtggaaggca agggaatata gtttgtgtaa gcatattgct tccttatgtt
      481 atattttagt aaagaattta ttttttacat tatagtcggc taaatgaagt ttactgtaaa
      541 aaaacgatac ttttttcgta agcgatgaga tgtggagcca gaaaaatggc tgcgttacgt
      601 cattgaactg tccttgaccc acgatattca gacagtcgtt gtatcttaag ttgacattac
      661 ccgctgacgt cgatattccc ttggtgagag ataacccaac gcactatgcg gatggttttc
      721 attgtaatgc tcgatcgcca ctgcaaggtt atgcaatgcc gttcttggtt tcggcatgaa
      781 cgcgatgcag tcttccttca tcgttttcac gaaccgttct gctattccat tactctgagg
      841 gctactgact gccgtcgtgc agggctccag attcaactct ctggcgaact gccgtgtttc
      901 atgagccgta taagccgaac cgttatcggc cactggattg gccgttccgg tcctttatcg
      961 ccatatcgct gacatgacat cctgcaccgt tgctttgtca taatctcccg tgcctgctgc
     1021 ccagtctatg gcttcacgat cgcaatatgg aacgtaacgc tgtcatcggg agaatcgtca
     1081 atgcggcatt cg
//
LOCUS       DAJVUJ010000323         1056 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.327, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000323 DAJVUJ010000000
VERSION     DAJVUJ010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.327"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..1033
                     /locus_tag="OQQ48_004291"
     rRNA            <1..1033
                     /locus_tag="OQQ48_004291"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          261 a          247 c          330 g          218 t
ORIGIN      
        1 actccgtgcc agcagccgcg gtaatacgga gggtgcaagc gttaatcgga attactgggc
       61 gtaaagcgca cgcaggcggt ttgttaagtc agatgtgaaa tccccgggct caacctggga
      121 actgcatctg atactggcaa gcttgagtct cgtagagggg ggtagaattc caggtgtagc
      181 ggtgaaatgc gtagagatct ggaggaatac cggtggcgaa ggcggccccc tggacgaaga
      241 ctgacgctca ggtgcgaaag cgtggggagc aaacaggatt agataccctg gtagtccacg
      301 ccgtaaacga tgtcgacttg gaggttgtgc ccttgaggcg tggcttccgg agctaacgcg
      361 ttaagtcgac cgcctgggga gtacggccgc aaggttaaaa ctcaaatgaa ttgacggggg
      421 cccgcacaag cggtggagca tgtggtttaa ttcgatgcaa cgcgaagaac cttacctggt
      481 cttgacatcc acggaagttt tcagagatga gaatgtgcct tcgggaaccg tgagacaggt
      541 gctgcatggc tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc
      601 aacccttatc ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata
      661 aactggagga aggtggggac gacgtcaagt catcatggcc cttacgacca gggctacaca
      721 cgtgctacaa tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctcataaa
      781 gtgcgtcgta gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta
      841 atcgtggatc agaatgccac ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac
      901 accatgggag tgggttgcaa aagaagtagg tagcttaacc ttcgggaggg cgcttaccac
      961 tttgtgattc atgactgggg tgaagtcgta acaaggtaac cgtaggggaa cctgcggttg
     1021 gatcacctcc ttaccttaaa gaagcgtact ttgcag
//
LOCUS       DAJVUJ010000324         1044 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.328, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000324 DAJVUJ010000000
VERSION     DAJVUJ010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.328"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..>1044
                     /locus_tag="OQQ48_004292"
     CDS             <1..>1044
                     /locus_tag="OQQ48_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCS1954807.1"
                     /translation="EHADGSVTQSQFDAVGRLRTQTDAAGRTTEYSPDVVTGLITRIT
                     TPDGRASAFYYNHHSQLTSATGPDGLEIRREYDEWGRLIQETAPDGDIIRYRYDNPHS
                     DLPCATEDATGSRKTMTWSRYGQLLSFTDCSGYVTRYDHDRFGQMTAVHREEGLSQYR
                     AYDSRGQLIAVKDTQGHETRYEYNAAGDLTAVIAPDGSRNGTQYDAWGKAIRTTQGEL
                     TRSMEYDAAGRVIRLTSENGSHTTFRYDVLDRLIQETGFDGRTQRYHHDLTGKLIRSE
                     DEGLVTHWHYDEADRLTHRTVNGETAEQWQYDKRGWLTDISHLSEGHRVTVHYRYDEK
                     GRLTGERQTVHHPQ"
BASE COUNT          260 a          306 c          317 g          161 t
ORIGIN      
        1 gaacacgcgg acggcagcgt cacgcagagt cagtttgacg ccgtgggcag gctcaggaca
       61 cagacggatg ccgcaggccg gacaacagaa tacagcccgg atgtggtgac gggcctcatc
      121 acgcgcatca ccacgccgga tggcagggca tcggcgtttt actataacca ccacagccag
      181 ttaacgtcag ccaccgggcc tgacgggctg gaaatacgcc gggaatatga tgaatggggc
      241 cgtctgattc aggaaactgc ccctgacggc gatatcatcc gctaccgtta tgataatcca
      301 cacagtgact taccctgcgc aacggaagat gccaccggta gccggaaaac catgacgtgg
      361 agccgttacg gtcagttgct gagcttcacc gactgttccg gttatgtaac ccgttatgac
      421 catgaccgct tcgggcagat gacggcggtg caccgcgagg aagggctgag tcagtaccgc
      481 gcatacgaca gccgtggaca gttaattgcc gtgaaagaca cgcagggcca tgaaacgcgg
      541 tatgaataca acgccgccgg tgacctgacc gccgtcattg ccccggacgg cagcagaaac
      601 gggacacagt acgatgcgtg gggaaaggcc atccgtacca cgcagggcga gctgacgcgc
      661 agtatggaat acgatgctgc aggacgggtc atccgcctga ccagtgaaaa cggcagccac
      721 accaccttcc gttacgatgt actcgaccgg ctgatacagg aaaccggctt tgacggccgc
      781 acacagcgtt atcaccacga cctgaccggc aaacttatcc gcagcgagga tgaggggctg
      841 gtcacccact ggcactatga cgaagcagac cgcctcacgc accgcaccgt gaatggcgaa
      901 accgcagagc agtggcagta tgacaaacgt ggctggctga cagacatcag ccatctcagc
      961 gaagggcacc gggtgacggt gcattacagg tatgatgaga aaggccggct gaccggtgag
     1021 cgccagacgg tgcatcaccc gcag
//
LOCUS       DAJVUJ010000325         1035 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.329, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000325 DAJVUJ010000000
VERSION     DAJVUJ010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.329"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..>1035
                     /locus_tag="OQQ48_004293"
     CDS             <1..>1035
                     /locus_tag="OQQ48_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCS1954808.1"
                     /translation="TDIALPGPLPFILSRTYSSYRTKTPAPVGSLGPGWKMPADIRLQ
                     LRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLWLVRGGVAKLDEGHRLAALWQALP
                     EELRLSPHRYLATNSPQGPWWLLGWCERVPEADEVLPAPLPPYRVLTGLVDRFGRTQT
                     FHREAAGEFSGEITGVTDGAGRHFQLVLTTQAQRAEEARQQAISGGTEPSAFPDTLPG
                     YTEYGRDNGIRLSAVWLTHDPEYPENLPAAPLVRYGWTPRGELAAVYDRSNTQVRSFT
                     YDDKYRGRMVAHRHTGRPEIRYRYDSDGRVTEQLNPAGLSYTYQYEKDRITITDSLNR
                     REVLHTQGEAG"
BASE COUNT          205 a          322 c          337 g          171 t
ORIGIN      
        1 aaccgacatc gccctgcccg gcccgctgcc gttcatcctc tcccgcacct acagcagtta
       61 ccggacaaaa acgcccgcgc cggtggggag cctcggcccc ggctggaaaa tgcctgcgga
      121 tatccgctta cagctgcgcg ataacacact gatactcagt gataacggcg gcagaagcct
      181 gtattttgag cacctgtttc ccggtgagga cggttacagc cgcagcgagt cactgtggct
      241 ggtgcgcggc ggcgtggcga aactggatga aggtcaccgg ctggccgcac tctggcaggc
      301 gctgccggaa gaactccgct taagtccgca tcgttatctg gcgacaaaca gtccgcaggg
      361 gccgtggtgg ctgctcggct ggtgtgagcg ggtgccggaa gcggatgagg tgctgcctgc
      421 gccgctgccg ccgtaccggg tactgaccgg gctggtggac cgcttcgggc gcacacagac
      481 gttccaccgc gaagccgccg gtgaattcag cggcgaaatc accggcgtga cggatggtgc
      541 cgggcgtcac ttccaactgg tactgaccac gcaggcgcag cgggcagaag aagcccggca
      601 gcaggccatt tccggcggga cggaaccgtc cgcttttcct gataccctgc cgggttacac
      661 cgaatatggc cgggacaacg gcatccgtct gtctgccgtg tggctgacgc acgacccgga
      721 atacccggag aatttacctg ccgcgccgct ggtgcgctat ggctggacgc cgcgcggcga
      781 actggcggca gtgtatgacc gcagcaatac acaggtgcgt agctttactt acgatgataa
      841 ataccggggc cggatggtgg cgcaccgtca cacgggccgg ccggaaatcc gttaccgtta
      901 cgacagcgac gggcgggtga cagaacagct taacccggca ggcttaagct acacgtatca
      961 gtatgagaag gaccgcatca ccatcaccga cagcctgaac cgccgtgaag tgctgcacac
     1021 gcagggtgaa gccgg
//
LOCUS       DAJVUJ010000326         1009 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.330, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000326 DAJVUJ010000000
VERSION     DAJVUJ010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.330"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            201..395
                     /gene="bfd"
                     /locus_tag="OQQ48_004294"
     CDS             201..395
                     /gene="bfd"
                     /locus_tag="OQQ48_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HCS1954809.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            468..944
                     /gene="bfr"
                     /locus_tag="OQQ48_004295"
     CDS             468..944
                     /gene="bfr"
                     /locus_tag="OQQ48_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HCS1954810.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGTPNLQDLGKLGIGEDVEEMLQSDLRLEQEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(941..>1009)
                     /locus_tag="OQQ48_004296"
     CDS             complement(941..>1009)
                     /locus_tag="OQQ48_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCS1954811.1"
                     /translation="PFGPFLAAAGFVIGWDSLLIGR"
BASE COUNT          302 a          205 c          231 g          271 t
ORIGIN      
        1 gctaaagttc tgggttaatt acacgttaat tagttttgaa ttgaaaaggg cgcttcggcg
       61 ccctttttgc tgcccgtaat ttgagctcga aatatttagt cgtaattttg ctcgctgaac
      121 tatggcgcaa ataaaactgt gctattgtaa tcataatcat tctcatttac actttgcgca
      181 gaatttgaac gggagcaatc atgtacgttt gtttgtgtaa tggtgtaagt gacaaaaaaa
      241 ttcgccaggc agtccgtcag tttcatccgc aatctttcca acaattgcgt aagtttattc
      301 ctgtgggaaa tcaatgcggt aagtgcattc gcgccgcgcg cgaagtgatg caggacgagt
      361 tgatgcagat gccagaattt aaagagattg cctgaagacc acgcttattt tttgacatcc
      421 cgctagcccc atctacgctc taatagtgga agcggaggga ctataaaatg aaaggtgatg
      481 ttaaaatcat aaattatctc aataaactat tgggaaatga gcttgtcgca atcaatcagt
      541 attttctcca tgcccggatg tttaaaaact ggggcctgat gcgtctcaat gacgtcgaat
      601 accatgaatc catcgatgag atgaaacacg ctgataaata tatcgagcgt attttgtttt
      661 tagaaggaac ccccaactta caggatttag gcaaactggg gattggtgaa gatgtcgagg
      721 agatgctgca atccgacctg cgattagaac aggaaggcgc gaaggatctg cgtgaggcta
      781 tcgcatatgc cgacagcgtt catgactatg ttagccgcga tatgatgatc gagatccttg
      841 ctgatgaaga aggtcatatc gactggctcg aaactgaact ggatctcatc ggtaaaattg
      901 gcctacaaaa ttacctgcaa tcacaaatta aagttaaaga ttaacggcct atcagcaaac
      961 tatcccagcc tataacgaaa cccgcagccg ccagaaatgg cccaaaagg
//
LOCUS       DAJVUJ010000327         1009 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.331, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000327 DAJVUJ010000000
VERSION     DAJVUJ010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.331"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            16..1009
                     /locus_tag="OQQ48_004297"
                     /pseudo
     CDS             16..1009
                     /locus_tag="OQQ48_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          348 a          134 c          208 g          319 t
ORIGIN      
        1 ttatagacgc tatatatggg tagtaatata catggaatta gttgcactgc aaataattat
       61 ttgaaacagg cctggaacga tataaaaaat gagtacgaaa aaaatcaaac atattcaatc
      121 acgctttttg aaaacacact ggtgtgtttt atgcggttat acaatgaact cagacgtaaa
      181 gtaaatgaag aggatactcc atgtctggaa tgtgaatcac tagaaaaaga atttgaggaa
      241 atgcagaatg ataatgatct atcattattt atgagaacat tgcgtactaa tgatacacaa
      301 atttattcag ggtttcagga ggtattacat atactataca atatgttcaa aatgttgata
      361 ttgttagagt gtccttgccg ggcagaggtt cagagtctat cacagatttt aaaggttatt
      421 attggtatgg ctttatggaa tatattgaaa acattactgc gtgtgatgat gttttttctg
      481 agtattgttt agatgatgaa aatatgagta tccagccaga acagataaat acgccgggaa
      541 tatctgattt agatactggt attgatttgt ctggcatatc ttttattcag agtgaaatta
      601 ataaaacata tggattaaaa tacgctccag tagatggcga tggatattgt ctgttaagag
      661 ccatactggt tttaaaagaa catgagtatt catgggcgct gggcggccat aagacgcaaa
      721 agcaagtata cgaagagttt attaaaattg ttgataaaca aacgatcgag gctcttgttg
      781 atacggcatt ctataatctc agggaagatg taaagacgtt atttggcgtt aatctacaat
      841 ctgacaacaa aattcagggg cagggtggtt ttttatcatg gagttttttg tcttttaaaa
      901 aagaatttat tgatagttgc ttgaatgaca aaaaatgcat actgcattta cccgaattta
      961 tatttaatga taacaaggta cgccttgttt tagacaccga cccagagca
//
LOCUS       DAJVUJ010000328         1000 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.332, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000328 DAJVUJ010000000
VERSION     DAJVUJ010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.332"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            69..599
                     /gene="cybB"
                     /locus_tag="OQQ48_004298"
     CDS             69..599
                     /gene="cybB"
                     /locus_tag="OQQ48_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HCS1954812.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            844..>1000
                     /locus_tag="OQQ48_004299"
     CDS             844..>1000
                     /locus_tag="OQQ48_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1954813.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASK"
BASE COUNT          267 a          210 c          229 g          294 t
ORIGIN      
        1 aatatttaat gaaattttga gaaaagcccg ttatgttaac ggaaaattat gttaaagcag
       61 gaaatgttat ggaaaataaa tactcaaggt tacaaatcag cattcactgg ctggtctttt
      121 tgctggttat cgcagcgtat tgcgcaatgg agtttcgggg tttcttccca cgtagcgatc
      181 ggccactcat caacatgatt catgtttcct gcggcatctc aattctcgtg ctgatggtcg
      241 ttcgtctttt gttaaggctg aaatacccaa ccccgccgat tatacctaag ccaaaaccga
      301 tgatgacggg actggcgcat ttgggacatt tggtgattta tctcctgttt attgcgctgc
      361 cagtgattgg tttggtgatg atgtataacc ggggcaaccc gtggtttgcg tttggtttga
      421 cgatgcctta cgcttcagag gccaatttcg aacgggttga tagcttaaag tcgtggcatg
      481 aaacgctggc gaatctggga tattttgtca tcgggttgca cgctgcggct gcactggcac
      541 accactattt ctggaaggac aacacacttc tacgcatgat gccgcgtaaa cgttcctgaa
      601 ggatatttaa agaaaacgcc tgtactaaaa ccgacccgtg gtacaggcga agaatacggg
      661 tctacatcgg aagcgcctat tatatttatt tgtatgacaa ataaaaccat aatccttgcc
      721 catacgtcca tctggcttat ttttaatcaa ttcacccgat ctttgatctc atcaacggta
      781 tcaaaataaa ccagcgtata acgttcaaat cgaacataag attcgataaa ccatggaggt
      841 tatatgaaaa aactggcact tattttgttt atgggaacgc ttgtttcctt ttatgccgat
      901 gccgggcgca aaccctgttc tggttcgaaa ggggggatct cacactgtac ggcaggcggc
      961 aaatttgtct gtaatgatgg ttctattagt gcctcgaaaa
//
LOCUS       DAJVUJ010000329          999 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.333, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000329 DAJVUJ010000000
VERSION     DAJVUJ010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..999
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.333"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..116
                     /locus_tag="OQQ48_004300"
     CDS             <1..116
                     /locus_tag="OQQ48_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCS1954814.1"
                     /translation="VSASSCKVEFRHGNMTLENPSPELLTVLIRELTGRGR"
     gene            113..463
                     /gene="tnpB"
                     /locus_tag="OQQ48_004301"
     CDS             113..463
                     /gene="tnpB"
                     /locus_tag="OQQ48_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCS1954815.1"
                     /translation="MISLPSGTRIWLVAGITDMRKSFNGLGEQVQHVLNDNPFSGHLF
                     IFRGRRGDMIKILWADADGLCLFTRRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSRLNALTML"
     gene            494..>999
                     /locus_tag="OQQ48_004302"
     CDS             494..>999
                     /locus_tag="OQQ48_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1954816.1"
                     /translation="MNQKYLIRIAELECQLRQKDQQLSLVEETEAFLRSALARAEEKI
                     EEDEREIEHLRAQIEKLRRMLFGTRSEKLRREVEQAEALLKQREQDSDRYSGREDDPQ
                     VPRQLRQSRHRRPLPEHLPREINRLEPEESCCPECGGELDYLGEVSAEQLELVSSALK
                     VIRTERVK"
BASE COUNT          254 a          255 c          284 g          206 t
ORIGIN      
        1 cagttagtgc cagctcctgc aaggtggagt tccgtcacgg taacatgacg ctggaaaatc
       61 cttcaccaga gctgctcaca gtgttgatcc gtgaactgac cgggagggga cgatgatctc
      121 actcccgtca ggcacccgca tctggctcgt tgctgggata accgatatgc gtaagtcttt
      181 caacgggctg ggtgaacagg tacagcatgt gctgaatgat aatcccttct ccggtcacct
      241 gttcatcttc cgtggccgac ggggtgacat gattaaaatc ctgtgggctg atgctgatgg
      301 tctgtgcctg ttcaccagac gcctggagga aggccagttt atctggcctg ctgtgcgtga
      361 cggcaaggta tccattaccc gctcgcaact ggcaatgctc ctcgataagc tggactggcg
      421 tcagccaaaa acatcccgcc ttaatgcact gacaatgttg taaaaatgtc atggccggat
      481 tataaaaacg gccatgaatc aaaaatacct cattcgcatt gcagaactgg aatgccagct
      541 ccgtcagaaa gaccagcaac tgagtctggt tgaagagacg gaggccttcc tgcgctctgc
      601 actggcccgc gccgaagaaa agatcgaaga agatgaacgg gaaatagaac atctgcgggc
      661 tcagatagaa aaactgcgcc ggatgctgtt cggtacccgt tctgaaaaac tgcgtcgtga
      721 agttgaacag gctgaggccc tgctgaaaca acgcgaacag gacagtgatc gttacagtgg
      781 gcgggaagac gatccgcagg ttccccgcca gttgcgacag tctcgtcatc gtcgcccgtt
      841 accggagcat ctgccccgcg aaataaatcg cctggagcca gaagaaagct gttgcccgga
      901 gtgtggcggt gagctggatt atctggggga agtcagcgca gaacaactgg aactggtgag
      961 cagcgctctg aaagtgatcc gcacagaacg ggtaaaaaa
//
LOCUS       DAJVUJ010000330          961 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.334, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000330 DAJVUJ010000000
VERSION     DAJVUJ010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..961
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.334"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(<1..638)
                     /gene="ospC4"
                     /locus_tag="OQQ48_004303"
     CDS             complement(<1..638)
                     /gene="ospC4"
                     /locus_tag="OQQ48_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC4"
                     /protein_id="HCS1954817.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKTNPNKATKALQKNILRVTNSSS
                     SGISEKHLDHCANTVKNFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSL
                     EHLDKVSRHYISEIIQKVHPLSSDERHLLSIIINSNFNFRHQSNSNLSNNILNIKSFD
                     KIQSENIQTHKNTYSEDIKEISNHDFVFFGVEISNHQEKLPLNKTHHTVDFGA"
BASE COUNT          284 a          151 c          172 g          354 t
ORIGIN      
        1 gcgccaaaat ctacagtatg atgtgtttta tttagcggga gtttttcttg atgattggaa
       61 atttcaaccc caaaaaaaac aaaatcgtgg ttagagattt ctttaatatc ctcagagtat
      121 gtattcttat gtgtttgtat attttcagac tgaattttat caaaggattt aatattaaga
      181 atattatttg acagattaga gttactttga tgtctgaagt taaaattaga attaataata
      241 atagaaagta agtgacgttc gtctgaagaa agaggatgaa ctttttgtat tatctcagag
      301 atatagtgcc tggacacctt atcgagatgc tcaagtgacc gaaatgatga ccgtgctcca
      361 gatggcgctt caatagaaag atttagtgat ttgaaactgg tcccttgaga aaacattttg
      421 ctataacttt gcgctgctat agacttcctt aaaaagtttt tcacagtatt agcacagtga
      481 tctaaatgtt tttcacttat gccagaagaa gatgagtttg ttacgcgtaa tatatttttt
      541 tgtaatgctt ttgttgcttt attcggattc gtttttccat caacaagatc aatattattc
      601 tgaacattaa tatgatttac tgcttcaggt attttcatcc cccccccaat tatgtgcgtt
      661 tttatctgtg gttatttaga taacattatc taactggaca ctttcttgta tgtattatag
      721 ctaaatgaac agattttaat atgttgtatg gttttcactc tgaaatcccc tgaatttaat
      781 cacgtttcct ttactgaagg caattttaac cgtagtatca attattaacc cttgggcttg
      841 atatccctct cctcttctgg gcacggggga tgtcagatta cttattacag aaaccaaaag
      901 aattgtaagc gtcatctcag tgtcgtctat ttcaatgtct gatgattggc gatgatagag
      961 t
//
LOCUS       DAJVUJ010000331          958 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.335, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000331 DAJVUJ010000000
VERSION     DAJVUJ010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..958
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.335"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(<1..209)
                     /locus_tag="OQQ48_004304"
     CDS             complement(<1..209)
                     /locus_tag="OQQ48_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000554745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1954818.1"
                     /translation="MHRIDTLTAVKDKFGPGKNGFTDGNLRTGRLATWLNSAMWNAIQ
                     EEICGVIEKAGIELNKEEHDQLYKA"
     gene            complement(196..786)
                     /locus_tag="OQQ48_004305"
     CDS             complement(196..786)
                     /locus_tag="OQQ48_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HCS1954819.1"
                     /translation="MDSLQDDYTKLLYGLMQPGPAWSDTDDVLDGLAPSLVRVHQRAD
                     ELVIEIDPGQSTELIERYEELYGLPDSCSPVGTQTLRQRQQRLEAKANVAGGINEQFF
                     LDQLEALGYTGVTIEQFQHLDASPDPEWGDRWRYFWRVTLPVDAGAQWQTCTDACNTP
                     IRTWGDTVAECVINKLCPSHTVVLFSYPDEDEDAQD"
     gene            complement(786..944)
                     /locus_tag="OQQ48_004306"
     CDS             complement(786..944)
                     /locus_tag="OQQ48_004306"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1954820.1"
                     /translation="MYLSRISEAISLSAGEVAHRLIVPSSDIDLGETELPVLGEITWQ
                     AYDPARSK"
BASE COUNT          227 a          282 c          216 g          233 t
ORIGIN      
        1 atagccttat atagctggtc gtgttcttct ttattcagtt ctatcccggc cttctcaatg
       61 acaccacaga tttcctcctg aatggcattc cacatggcac tgttcagcca ggtagcaaga
      121 cgtcctgtgc gaagatttcc gtcagtaaat ccgttcttac ccggaccaaa cttatctttt
      181 accgcagtca gcgtatcaat cctgtgcatc ttcatcctca tctggatagg aaaataaaac
      241 gacggtgtgt gacggacata atttattaat cacgcactcc gcaaccgtat ccccccacgt
      301 ccggatcggt gtgttgcagg cgtccgtgca tgtctgccac tgagcaccgg catccaccgg
      361 caacgtcaca cgccagaaat aacgccagcg atctccccat tccggatcgg ggcttgcatc
      421 caggtgctgg aactgttcga tcgttacgcc ggtatatccc agcgcctcaa gctgatccag
      481 gaagaactgc tcatttatgc caccagccac attggctttt gcttcaagac gttgctgacg
      541 ctggcgtaat gtctgggttc cgacagggga acaggaatca ggtaaaccat acaattcttc
      601 ataacgttca atcagctctg tggactgacc gggatcgatt tcaatcacca gttcatcagc
      661 ccgctggtga acacgcacaa gcgatggtgc cagaccgtca agtacatcgt cggtatctga
      721 ccatgcaggc cccggctgca tcagtccgta cagcagcttt gtataatcat cctgtaacga
      781 atccattatt tactccttgc cgggtcataa gcctgccagg tgatctcgcc gagcaccgga
      841 agctcggtct cccccaggtc aatatccgat gaagggacga ttaaccggtg ggccacttca
      901 ccagcagaca aactgatggc ctcactgatt ctggacagat acatacgccc ctctggca
//
LOCUS       DAJVUJ010000332          935 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.336, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000332 DAJVUJ010000000
VERSION     DAJVUJ010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..935
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.336"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..598
                     /locus_tag="OQQ48_004307"
     CDS             <1..598
                     /locus_tag="OQQ48_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA
                     acyltransferase"
                     /protein_id="HCS1954821.1"
                     /translation="GLEILEEIRYNNRNVIFLVPHGWSVDIPAMLLAAQGEKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHSREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHFLTIEVRPPMDACIASADNKMI
                     ARQMNKTVEILVGSHPEQYIWVLKLLKTRKSNEADPYP"
     gene            complement(752..>935)
                     /locus_tag="OQQ48_004308"
     CDS             complement(752..>935)
                     /locus_tag="OQQ48_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HCS1954822.1"
                     /translation="IYQPVYSPWVNHAERLWQALHDTITRNHQCRSMWQLLKKVRHFM
                     ETVSPFPGGKHGLAKV"
BASE COUNT          257 a          196 c          219 g          263 t
ORIGIN      
        1 aggcctcgaa attcttgagg aaattcgata caataacaga aatgtgattt ttctggttcc
       61 ccatggctgg agcgtggata ttcctgcaat gttgctggca gctcaggggg aaaaaatggc
      121 cgccatgttt catcagcaac gaaatccagt gattgattat gtctggaatt ctgtacggcg
      181 taaattcggg gggcgcttac attcccggga ggatgggata aaaccattta ttcagtcagt
      241 acgccaggga tactgggggt attaccttcc agatcaggat catggtcctg aatacagtga
      301 atttgctgat ttttttgcga cctataaagc gacattacca attattggac gtctgatgaa
      361 catcagtcag gctatgatta taccgctttt cccggtttat gatgaaaaaa aacatttcct
      421 gactattgaa gttcggccac caatggatgc atgcattgcc agcgcggaca ataaaatgat
      481 tgcccgacaa atgaacaaaa cagtggaaat tttggtgggg tcacatccgg aacagtatat
      541 ctgggtttta aaattgttaa aaacgcgcaa atcaaacgaa gcggacccgt acccttgaaa
      601 ttataaagta caggtatttc cactagttgt ttcttacagg ttaccaatcg aaacacatcc
      661 cccttccgtg tgttgcccgt ttggcacgga ggactgtgta gatgcagttc gttaaattta
      721 atttatgcct aaatagctgc gcttaatacc gctacacttt tgccagtcca tgtttgcctc
      781 cggggaatgg gctgacggtt tccataaaat ggcgaacttt tttcaacagt tgccacattg
      841 agcggcactg atgattacgc gttattgtgt cgtgaagtgc ctgccatagc cgttcagcat
      901 gattcaccca tggcgagtaa accggctgat aaatg
//
LOCUS       DAJVUJ010000333          928 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.337, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000333 DAJVUJ010000000
VERSION     DAJVUJ010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..928
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.337"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(33..212)
                     /locus_tag="OQQ48_004309"
     CDS             complement(33..212)
                     /locus_tag="OQQ48_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001039895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1954823.1"
                     /translation="MNKPQLAESQKTALLTEAESVIRPRGRAVRLNRETDESGEARGR
                     ASVFPESVRSVVYAM"
     gene            complement(212..835)
                     /locus_tag="OQQ48_004310"
     CDS             complement(212..835)
                     /locus_tag="OQQ48_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HCS1954824.1"
                     /translation="MYMSLAQCPGFLFAHREECTVEIKKIINPRYTESGAVDCDVFFD
                     DRDQAVPYTATADDVAPTGQQIWQELQSGKWGEIAPFTVTPEMLEAAREARRQEIEAW
                     RAEQEAKPFTFEWNGRIWNAGPDSLGRLSPVVMLAKSVTAQTHMAWSDADNQQVKLSM
                     PELEELAAAMVQAQVDRNDEIYRRQREMKEELSGLDDLASIRAFDVE"
     gene            complement(786..>928)
                     /locus_tag="OQQ48_004311"
     CDS             complement(786..>928)
                     /locus_tag="OQQ48_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1954825.1"
                     /translation="GTYIASPQFFITGCSEHSLPGSYCALSGGPDVHVSGAMPGLFIR
                     TS"
BASE COUNT          201 a          286 c          218 g          223 t
ORIGIN      
        1 ctgaaaacgg ctcacaactg caccattcat aatcacatcg cataaaccac agaccggact
       61 gactccggaa aaacagaggc ccgcccccgg gcctccccgg attcatccgt ttccctgttc
      121 agcctgacag cacgcccccg cggccggatg acagactccg cttcggtaag caaagcggtc
      181 ttctgtgatt ccgccagttg cggcttattc attactcaac gtcaaacgcc cgaattgaag
      241 ccaaatcatc cagaccgctc agctcctctt tcatttcacg ctgacggcga taaatctcat
      301 cgttgcgatc gacctgcgcc tgcaccattg ctgccgccag ttcttccagt tccggcatcg
      361 acagtttcac ctgctgatta tcggcatcgc tccacgccat atgtgtttgt gctgtgacag
      421 attttgccag catgactacc ggggacaggc ggcccagtga gtcgggacca gcattccaga
      481 tacgaccgtt ccattcaaac gtgaacggct tcgcctcctg ttctgcgcgc catgcttcaa
      541 tttcctgacg tctggcctct ctggccgctt ccagcatttc tggtgtcaca gtgaatgggg
      601 ctatctcacc ccatttgccg ctttgcagtt cctgccagat ttgctgaccc gtcggagcga
      661 catcatcagc ggtggctgtg taggggactg cctggtccct gtcgtcaaaa aaaacgtcac
      721 agtctactgc gccactttcg gtataacggg gattaatgat ttttttaatt tccacggtgc
      781 attcctcacg atgtgcgaat aaaaagcccg ggcattgcgc cagagacatg tacatccggc
      841 cccccggaca gggcgcaata tgaccccggt aatgaatgct ctgaacatcc cgtaatgaaa
      901 aattgtgggg atgctatata cgttccgg
//
LOCUS       DAJVUJ010000334          920 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.338, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000334 DAJVUJ010000000
VERSION     DAJVUJ010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..920
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.338"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..648
                     /gene="ospC4"
                     /locus_tag="OQQ48_004312"
     CDS             <1..648
                     /gene="ospC4"
                     /locus_tag="OQQ48_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC4"
                     /protein_id="HCS1954826.1"
                     /translation="KTDVPIFNTKDMRLGIGLHLIDFIRKSKDQGFREFCYNKNIDPV
                     SLDRIINFVFQLEYHIPRMLSTDNFKKIKLRDISLEDAIKASNYEEINNKVTDKKMAH
                     QALAYSLGNKKADIALYLLSKFNFTKQDVAEMEKMNNNRYCNLYDVEYLLSKDGANYK
                     VLEYFINNGLVDVNKKFQKANSGDTMLDNAMKSKKPKMIDFLLKNGAILGKRFEI"
BASE COUNT          327 a          141 c          179 g          273 t
ORIGIN      
        1 aaaactgatg ttccaatatt taacactaaa gatatgaggc ttggaattgg gttgcatctt
       61 attgatttta taagaaagag taaagatcaa ggattcagag agttttgtta taacaaaaat
      121 attgatcctg ttagtttgga tagaattata aactttgtgt ttcagcttga gtatcatata
      181 ccaagaatgc taagtacaga taacttcaaa aaaataaaac tcagagacat atctttggag
      241 gatgcgatta aagcatctaa ttatgaagag attaacaata aagtgactga taaaaaaatg
      301 gctcatcagg cccttgctta ttctttaggc aataaaaaag ctgatatagc actttattta
      361 ctatctaaat ttaattttac aaaacaagat gtcgcagaga tggagaaaat gaacaacaat
      421 agatattgta atctgtatga tgttgaatac ttacttagta aagatggtgc caactataaa
      481 gtgctagaat attttatcaa taatggattg gttgatgtaa acaaaaaatt tcaaaaagca
      541 aatagtgggg acactatgct tgacaatgca atgaaaagca aaaaaccaaa aatgattgat
      601 tttttattaa aaaatggagc aatattaggc aaacgatttg agatatgacc taaaagctcg
      661 gccaaataat aatgtcgtcg attatcgaag tcatcatgag tgattctgtc cccacagtgc
      721 agcatcagca tgacagtgaa ttgtctggcg tggtccattt aatgagatgg agatggtccc
      781 tctttcctaa atagctgtgc ctaataccgc tacacttttg ccagcccatg tttgcctccg
      841 gggaatgggc tgacggttcc ttcaaaatcc attgcacgca gttatgtcgg agcgggaact
      901 tgactcctgt ctttacggga
//
LOCUS       DAJVUJ010000335          914 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.339, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000335 DAJVUJ010000000
VERSION     DAJVUJ010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..914
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.339"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..>914
                     /gene="tuf"
                     /locus_tag="OQQ48_004313"
     CDS             <1..>914
                     /gene="tuf"
                     /locus_tag="OQQ48_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCS1954827.1"
                     /translation="AHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHIL
                     LGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKAL
                     EGDAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGII
                     KVGEEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAK
                     PGTIKPHTKFESEVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMP
                     GDNIKMVVTLIHPIAMDDGLRFAIREGGR"
BASE COUNT          212 a          230 c          264 g          208 t
ORIGIN      
        1 gcacacgtag actgcccggg gcacgccgac tatgttaaaa acatgatcac cggtgctgct
       61 cagatggacg gcgcgatcct ggtagttgct gcgactgacg gcccgatgcc gcagactcgt
      121 gagcacatcc tgctgggtcg tcaggtaggc gttccgtaca tcatcgtgtt cctgaacaaa
      181 tgcgacatgg ttgatgacga agagctgctg gaactggttg aaatggaagt tcgtgaactt
      241 ctgtctcagt acgacttccc gggcgacgac actccgatcg ttcgtggttc tgctctgaaa
      301 gcgctggaag gcgacgcaga gtgggaagcg aaaatcctgg aactggctgg cttcctggat
      361 tcttacattc cggaaccaga gcgtgcgatt gacaagccgt tcctgctgcc gatcgaagac
      421 gtattctcca tctccggtcg tggtaccgtt gttaccggtc gtgtagaacg cggtatcatc
      481 aaagttggtg aagaagttga aatcgttggt atcaaagaga ctcagaagtc tacctgtact
      541 ggcgttgaaa tgttccgcaa actgctggac gaaggccgtg ctggtgagaa cgtaggtgtt
      601 ctgctgcgtg gtatcaaacg tgaagaaatc gaacgtggtc aggtactggc taagccgggc
      661 accatcaagc cgcacaccaa gttcgaatct gaagtgtaca ttctgtccaa agatgaaggc
      721 ggccgtcata ctccgttctt caaaggctac cgtccgcagt tctacttccg tactactgac
      781 gtgactggta ccatcgaact gccggaaggc gtagagatgg taatgccggg cgacaacatc
      841 aaaatggttg ttaccctgat ccacccgatc gcgatggacg acggtctgcg tttcgcaatc
      901 cgtgaaggcg gccg
//
LOCUS       DAJVUJ010000336          910 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.340, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000336 DAJVUJ010000000
VERSION     DAJVUJ010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..910
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.340"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(<1..>910)
                     /locus_tag="OQQ48_004314"
     CDS             complement(<1..>910)
                     /locus_tag="OQQ48_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HCS1954828.1"
                     /translation="GITGGNVFFSCLVFVFFYFAILMTVSADMPLVGCVLTLSLLVLL
                     IFMAAKGLRYQALMTSLNGVRFSFNCSMKGFWWVTFFLPILMAIGMGTVFFISTKMLH
                     ANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFKVKLDTAYCI
                     KYAILAFLALLPFLAVAGYIIFEQILNAYDSSVYANDDIENLQQFMEMQRKMIIAQLI
                     YYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCALVVISGITGG
                     LAYPLLKIWMIDWQAKNTYLLGDLDDL"
BASE COUNT          347 a          206 c          139 g          218 t
ORIGIN      
        1 aggtcatcca aatcgcccag caaatacgta ttttttgcct gccagtcaat catccatatt
       61 ttcagcagtg gataagccag accgcccgta atcccggata tcactaccaa cgcacacatg
      121 cgataaagca taccgtggta cgttaaagtt gagcgaaaac gaatacgccc atcattcagt
      181 gacaggttac tcataaaatg atttcgcaac gacaccgtta aataactggt gctaacagca
      241 atcccaaaat aatagattaa ctgcgcgatt atcattttac gttgcatttc cataaattgc
      301 tgtaaattct caatatcatc atttgcatac acagaactat catacgcatt taatatttgt
      361 tcgaagataa tataaccagc aacagcgaga aaaggtaata aagctaaaaa tgcgaggatg
      421 gcatatttta tacaatacgc agtatctaat ttcaccttga aacgatgtat accgaaactg
      481 gtattgctcc agagaaaact cattaccagg ctatataaag taccattaaa aataccaatg
      541 gaaacaatac caactattgc catcagaacc acagatataa taacactact tgaactattg
      601 gcatgtagca tctttgtcga gataaagaaa acagtcccca tcccaatggc cattaaaatc
      661 ggcaagaaaa aggttaccca ccagaaccct ttcatagagc aattaaaact aaatcttacg
      721 ccgttgagac tagtcatcaa ggcctgataa cgcagtcctt ttgctgccat aaatataagc
      781 aaaaccaaca gtgacaaagt caaaacacag ccaacaagcg gcatatctgc tgacactgtc
      841 attaagattg cgaaatagaa aaaaacaaaa acaagacaac taaaaaaaac attcccacca
      901 gtaattccat
//
LOCUS       DAJVUJ010000337          891 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.341, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000337 DAJVUJ010000000
VERSION     DAJVUJ010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..891
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.341"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(73..261)
                     /locus_tag="OQQ48_004315"
     CDS             complement(73..261)
                     /locus_tag="OQQ48_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HCS1954829.1"
                     /translation="MAFKHYDVVRAAPPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPE"
     gene            522..857
                     /gene="iraM"
                     /locus_tag="OQQ48_004316"
     CDS             522..857
                     /gene="iraM"
                     /locus_tag="OQQ48_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HCS1954830.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
BASE COUNT          311 a          203 c          141 g          236 t
ORIGIN      
        1 ccggtgcatt tagtcccact gccagactgt cttttatggc ctggaatgcc gggccagtac
       61 gatgacgtag tatcactccg gctcagttgc accactgacc accacatcac cttctgctgc
      121 aatcgcctgc atcagggtat aaggggttat ggccaccgga ctaccaaacg gctgccagcc
      181 ctctttcagt ttatgtgtca gcttttccgc aagatctgac ggcggcgccg ccctgacaac
      241 atcatagtgt ttaaatgcca tggttctttc caccatctga aaaataattc tttaaaatac
      301 ctgacatgta atacagaaaa aacacaaaac cataccttaa ataaaaacct gattatcaag
      361 cagatatgca tggataaact acaagacgag atataaacca ccctgcattt aaataaacaa
      421 taaacaacat cagaaaaata attctgctct atggtttaca ttcaaaaata tcatttatac
      481 ttttcagaac atcaccagca aggcataaac aaggaaacta aatgaagtgg attgtgattg
      541 atacagttat ccagccatca tgcggaatat ctttttcagt catatggagt aaaataaaat
      601 taataatctg gtatcaatcg gatgctttct tacctcctga aagtatattt acactgactc
      661 acacaggcat catgctcaat aacaaagtgc tacctgtaac catttacaac gtagtaccat
      721 tcaataaaac attctggaat ttaatcaaaa acagccagga atgccctaca aatacagata
      781 acgtattgaa tgaatgcttt aataaccgtt gcactctgca aatatgtcct tatggactaa
      841 aacaacaaag tccataagga gtttactcac atctgacaaa atcaatataa a
//
LOCUS       DAJVUJ010000338          886 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.342, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000338 DAJVUJ010000000
VERSION     DAJVUJ010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..886
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.342"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            87..886
                     /locus_tag="OQQ48_004317"
                     /pseudo
     CDS             87..886
                     /locus_tag="OQQ48_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415843.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
BASE COUNT          254 a          208 c          215 g          209 t
ORIGIN      
        1 atttagggtc taatcaactt aatgatcaat atctatagtg atatcaccaa ccgttaaaac
       61 gaaccgacaa ctccatgagg aacggaatga tgaataataa agtcagcttc actaacagca
      121 ataatccaac catctctttg tctgcagtga tctatttccc tccaaaattt gatgaaaccc
      181 gtcagtatca ggcaattgtg gtatctcacc caggtggggc gttaaagaac aaacggccgg
      241 aacctatgcc aaaaaactgg cggaaaaggg atttgtcact attgcttatg acgcatctta
      301 tcaaggtgaa agtggcggcg agccgcgtca gttagaaaac ccatacattc gtactgaaga
      361 catcagtgcc gtgattgatt acctgacaac gctttcttat gtcgacaata cccgaattgg
      421 tgcgatggga atttgtgccg gtgcaggata tacagccaat gctgcaattc aggatcgccg
      481 catcaaggcc atcggtaccg ttagtgcggt caatattggt tcgatgttcc gtaatggttg
      541 ggaaaacaat gtgaaatcta ttgatgccct gccatacgtt gaagcgggtt caaacgcccg
      601 aaccagcgat attagcagcg gagaatatgc cgttatgcca ctggcaccaa tgaaggagtc
      661 cgatgcgccg aacgaagaac tgcgtcaggc ctgggagtat taccacaccc ctcgcgcgca
      721 gtatccaaca gcaccgggtt acgctacttt gcgcagcctt aaccagatta ttacctttga
      781 tgcttaccat atggcggaag tgtacctgac tcagccgatg cagattgtgg cgggcagcca
      841 ggcagggagc aaatggatga gtgacgatct gtacgatcga gcatca
//
LOCUS       DAJVUJ010000339          872 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.343, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000339 DAJVUJ010000000
VERSION     DAJVUJ010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..872
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.343"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(32..112)
                     /gene="csgC"
                     /locus_tag="OQQ48_004318"
                     /pseudo
     CDS             complement(32..112)
                     /gene="csgC"
                     /locus_tag="OQQ48_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="involved in autoagglutination of curliated cells;
                     not involved in production of curli fibers; incomplete;
                     partial in the middle of a contig; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
     gene            complement(171..626)
                     /gene="csgA"
                     /locus_tag="OQQ48_004319"
     CDS             complement(171..626)
                     /gene="csgA"
                     /locus_tag="OQQ48_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin major subunit CsgA"
                     /protein_id="HCS1954831.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGGGNNSGPNSE
                     LNIYQYGGGNSALALQTDARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSAT
                     LDQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
     gene            complement(667..>872)
                     /gene="csgB"
                     /locus_tag="OQQ48_004320"
     CDS             complement(667..>872)
                     /gene="csgB"
                     /locus_tag="OQQ48_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="CsgB; functions as a nucleator in the assembly of
                     curli (coiled surface structures) on the cell surface;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HCS1954832.1"
                     /translation="GQSNNLAYIDQAGSANDASISQGAYGNTAMIIQKGSGNKANITQ
                     YGTQKTAIVVQRQSQMAIRVTQR"
BASE COUNT          229 a          218 c          192 g          233 t
ORIGIN      
        1 gaacaaatgg tagtgtccgc tattgccagt acacctcata cacatcccct tgctgggtcg
       61 tattaaaggt tatctgactg gaaagtgccg caaggagtaa taacgcattc atattcttct
      121 cccgaaaaaa aacagggctt gcgccctgtt tctgtaatac aaatgatgta ttagtactga
      181 tgagcggtcg cgttgttacc aaagccaacc tgagtcacgt tgacggagga gttagatgca
      241 gtctggtcaa ctgcagcacc gttgccacca ccgaactgtt taaccgtcat ttcagaattt
      301 ttgccgttcc actgatcaag agtagcgcta ttaccgaagc cacgttgggt cagatcgatt
      361 gagctgtcat ctgagccctg accaacatct gcaccattac cgccgccatg ctgggtaata
      421 gtcaagtcag agttacgggc atcagtttgc agagcaagtg cagagttacc gccaccgtac
      481 tggtaaatgt tcagctcaga atttgggccg ctattattac cgccaccacc gtggttaccg
      541 ccgccgccgt actgaggaac aacacctgcc agagcgctac cggagaatac gattgctgca
      601 attgctgcta cttttaaaag tttcatgtaa aacccccatc ggattgattt aaaagtcgaa
      661 tggaaattaa cgttgtgtca cgcgaatagc catttgcgac tgtctctgca ctacaattgc
      721 cgttttttga gtaccatact gtgtaatatt tgctttatta ccagaacctt tctggataat
      781 catcgcagta ttaccataag caccttgcga aatactggca tcgttggcac tgcccgcctg
      841 atcaatatat gcaaggttat tagactggcc cc
//
LOCUS       DAJVUJ010000340          870 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.344, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000340 DAJVUJ010000000
VERSION     DAJVUJ010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..870
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.344"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..504
                     /locus_tag="OQQ48_004321"
     CDS             <1..504
                     /locus_tag="OQQ48_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HCS1954833.1"
                     /translation="YITQDPIGLKGGWNFYQYPLNPISEIDPQGLNPLILGVVALMSC
                     GLFSSGGVIQSGQQRRDQYGRKVNPEADKLTDEHKSGVNPGGNCSPQNLSDLQNEKNR
                     LCNQSRACAPQMLRKEILRRYEINLACASVRKQINNQCFGGGDKAHMEEENKAYKTAA
                     DCSGLIK"
     gene            501..>870
                     /locus_tag="OQQ48_004322"
     CDS             501..>870
                     /locus_tag="OQQ48_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1954834.1"
                     /translation="MKKIIFSNESAVYDELMIHFPCQPLPHISNDIIGLDELDVVYNF
                     FQKKQWNEIANNLKIKDDSYALELGITFLPEKVFCYYIPLYIYVSLFNKNDFWVFESD
                     FIQQYLCPEYRDYDDFLNFVF"
BASE COUNT          284 a          133 c          185 g          268 t
ORIGIN      
        1 tatatcactc aggacccaat tgggctgaag gggggatgga atttttatca gtatccgttg
       61 aatccgatct cagagataga cccacaaggg ttaaatccgt taattcttgg tgttgtagca
      121 ctgatgagtt gcggtttgtt ttcatcaggt ggtgttatcc aaagtgggca acaacgcaga
      181 gatcaatatg gacgtaaagt taatccagaa gccgacaaat taacagatga acataagtca
      241 ggtgtaaatc ctggaggtaa ctgtagtccc cagaacctga gtgacttaca aaatgaaaaa
      301 aacagattat gtaaccagtc tagagcttgc gctcctcaga tgctgagaaa agaaatattg
      361 agacggtacg agataaattt ggcgtgcgct tcggtcagaa agcagattaa taatcagtgc
      421 tttggtggtg gagataaggc ccatatggaa gaagagaata aagcatataa aacagcagct
      481 gattgcagtg gcttaataaa atgaaaaaaa taattttctc taacgagagt gcagtctatg
      541 atgaacttat gattcatttt ccatgccagc cattacctca tatttcaaat gatattatag
      601 ggctggatga actggatgtt gtatataatt ttttccaaaa gaaacaatgg aatgaaatag
      661 ccaataattt aaaaataaag gatgactctt atgctttaga gttaggaatt acattcctcc
      721 ctgagaaagt attctgctat tacatccctc tgtatattta tgtctcgttg tttaataaga
      781 atgatttttg ggtttttgaa tccgatttta ttcaacaata tttatgtcct gagtatcggg
      841 attacgatga ttttttgaat tttgttttta
//
LOCUS       DAJVUJ010000341          851 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.345, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000341 DAJVUJ010000000
VERSION     DAJVUJ010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..851
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.345"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            45..323
                     /locus_tag="OQQ48_004323"
     CDS             45..323
                     /locus_tag="OQQ48_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005028610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HCS1954835.1"
                     /translation="MSDAVYRRLILMKAMSNITDCSVPDINRMLRFMFGKKRRAYVLN
                     NGGLRMSYIFESALSLAELAIIQSSGALPSPPGVYVSVVLKESRNEGQ"
     gene            310..>851
                     /locus_tag="OQQ48_004324"
     CDS             310..>851
                     /locus_tag="OQQ48_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1954836.1"
                     /translation="MKASDKPRQLAVPFASTGDKNRIPDKATQQTRESGNAAYDSGFP
                     PVTMTAVSAGGIPPHGKDFNGLMYDITAAIRFAQAGGLYTYNAGFAGAIGGYAKGAIL
                     AGVATTAVWLNTTDDNLTDPEGSDSAGWVNLLEDPKRIFLRQKNNLSDLQNKGTVRDN
                     LQVYSKEQSDQRYVHREGDK"
BASE COUNT          218 a          196 c          239 g          198 t
ORIGIN      
        1 aggcaccgtt ttacaacgga aaatcggata cccggactgt tgacctgtct gatgctgtat
       61 accggcggct gatactgatg aaagccatgt cgaacattac tgactgttcc gttccggata
      121 ttaaccggat gctgagattt atgttcggaa aaaaacggcg agcttatgtt ctgaataatg
      181 gtgggctgag gatgagttac atctttgagt ccgcgctctc gttggcagaa ctggcgatta
      241 tccagtcgtc gggtgcactg ccgtccccgc cgggtgttta tgtttcagta gttttaaagg
      301 agtcccgtaa tgaaggccag tgataaaccc cgccagctgg cggtcccctt tgcgagtacc
      361 ggagataaaa accgtatccc ggacaaggcg acacagcaga ccagagagag cggtaatgct
      421 gcgtatgatt caggttttcc tccggtgacc atgacagcgg tctcagcggg aggtataccg
      481 ccacacggca aggattttaa cggtctgatg tacgatatta ccgcagcaat acggttcgcc
      541 caggctggcg gtttgtacac gtataatgcc ggttttgcgg gggccattgg tggatatgca
      601 aaaggagcca ttctcgccgg agtcgcaaca acagcggtct ggctgaatac cacagacgat
      661 aacctgaccg atcctgaagg ctccgacagt gcgggctggg taaatcttct tgaggatccg
      721 aaaaggatat tcctgcggca gaagaacaat ctgtcagacc ttcagaataa agggacggta
      781 cgggataatc ttcaggttta cagtaaagag cagtcagatc aacgctatgt tcatcgggaa
      841 ggcgataaaa t
//
LOCUS       DAJVUJ010000342          840 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.346, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000342 DAJVUJ010000000
VERSION     DAJVUJ010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..840
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.346"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..>840
                     /locus_tag="OQQ48_004325"
     CDS             <1..>840
                     /locus_tag="OQQ48_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCS1954837.1"
                     /translation="LVGYIAWLATAYRMSFSREINLGLAQHEFELFCQPLLNARSQQC
                     IGVEILLRWNNPRQGRISPDVFIPIAEEHHLIVPLTRYVMAETIRQRHVFPMSSQFHV
                     GINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITERDALLDVDYRIARELHRKNVK
                     LAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSDAVNSTVTDIIIALGQRLNIEL
                     VAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFPKWLAGSQPPPARHNGHITPIM
                     PLR"
BASE COUNT          194 a          214 c          217 g          215 t
ORIGIN      
        1 actggtgggc tacatcgcct ggctggcgac cgcttaccgg atgagctttt cccgcgaaat
       61 caatctgggc ctggcgcaac atgagttcga attgttctgt cagcctttgc ttaatgcgcg
      121 cagccagcaa tgtattggtg tagagatttt gctgcgctgg aacaatccgc gtcagggccg
      181 gatttcaccg gatgtgttta ttcctatcgc ggaagaacat catttaattg tgccactgac
      241 ccgctatgtg atggcagaaa ccattcgtca gcgccatgtt ttcccgatga gtagtcagtt
      301 tcatgttggc attaacgtcg cacccagcca ttttcgccgt ggtgtgctga taaaagatct
      361 caatcagtac tggtttagcg ctcacccgat tcagcaactg atcctcgaaa tcaccgaacg
      421 cgatgcctta ctggatgttg attatcggat tgcccgcgag ctacatcgta aaaacgtcaa
      481 actggcgatt gatgacttcg gcaccggcaa cagttcattt tcctggcttg aaacattacg
      541 tcctgacgtg ctgaaaattg ataagtcatt taccgcagct ataggttctg acgcggttaa
      601 ctcgacggtg accgatatca tcatcgcgct ggggcaaaga ctgaatattg aactggtggc
      661 ggagggcgtg gaaacgcaag aacaggcgaa gtatttgcgc cgtcatggcg tgcatatttt
      721 gcaagggtat ttgtacgcac agccgatgcc gctacgcgat tttcccaaat ggctggcggg
      781 cagccaaccg ccgcccgccc ggcataatgg acatatcacg cccattatgc cgttacgtta
//
LOCUS       DAJVUJ010000343          838 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.347, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000343 DAJVUJ010000000
VERSION     DAJVUJ010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..838
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.347"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(180..>838)
                     /locus_tag="OQQ48_004326"
     CDS             complement(180..>838)
                     /locus_tag="OQQ48_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCS1954838.1"
                     /translation="TGEIDTALWRSGDVLLGSLLAMLFTGIWPQRAFIHWRIQLAKSL
                     TEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIAPASKETRIPKSIYEGIQT
                     INRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQQILLSLVHALYEGNPQPV
                     FANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNMETAHQLELLSNLICRALRK
                     "
BASE COUNT          206 a          204 c          195 g          233 t
ORIGIN      
        1 gtttccattt ttatacccct gataatgtga gagtcggatt cgtttaagca accgctatcc
       61 gttagagcca acattactca tagctgccgc agatgacaat gcttttatcc cttttcacta
      121 tcataccctt tatccttgct gaatcgaagc agcagcaaga tgattctgta gttcaggaat
      181 tatttgcgca aggcccggca aatcagattc gatagcaact caagctgatg tgccgtttcc
      241 atgttcagcc acacataacc atagattggt gtttccacaa ccttcaggtc atggtggtta
      301 ttgagcaact gacgcagctc ttccactgca tcgttcaatt tttccgtatt ggcaaaaacc
      361 ggctgcggat taccttcgta caacgcatga acaaggctca gcagtatttg ctgcatcatg
      421 tgctgggtat cacgaagttt ttgcgcgttc aataacacga aatggctggg gcgcgtggcc
      481 cagtacgcat tgatttgcaa ctccagcata caaaccagat tgcggttaat ggtctggata
      541 ccttcatata tcgattttgg aatacgggtt tctttgctgg cgggcgcaat cagcccacgc
      601 attttcacgg catcggtcag gagtttttgt agatggcttt ccagacgtgg gcgttcgagt
      661 aagttcggtg agaatgcaga ttgatagacc cgattatact cggtcagact tttcgccagt
      721 tgaatgcgcc agtggatgaa cgcccgttgt ggccagatac cggtaaacaa cattgccagt
      781 aaagagccga ggagcacatc gccgcttcgc cataacgccg tatcaatttc acctgtcg
//
LOCUS       DAJVUJ010000344          793 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.348, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000344 DAJVUJ010000000
VERSION     DAJVUJ010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..793
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.348"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
BASE COUNT          154 a          248 c          195 g          196 t
ORIGIN      
        1 cgtagggacg gatcggtggc tgcccatgtg gcaagcttgc gttgcatttc gctgattatc
       61 aaaggtcttc acctcgttag gtcagttaat tcacgtcgca aacacattca aactgcttcc
      121 cttcgccatg taatgggctt tccccatcgc ggactactac ggaagctccg ccagccagcg
      181 cgtcatcgga gccatgcccc cttaacatcc gtcgctgacc ttccccggtt tacctgcctg
      241 gactcaggca tactgaggag gctgcccgtc gcactcttta tccttgcttg ccgcaagttg
      301 gcagaagtca gcaacgcaag cgtgatagac gctgctgccc cggtgtttcg catacatgtc
      361 aaaacacctt cgaccggcag tgcttacgta tcactgccag ttcctcctgc acggcctgtc
      421 agatcacgta ggccgtggtg acgttttcaa cccacagagg cggattaacg ggttcatgtt
      481 cttcagcctt tcagtactta accttgagga tcatctcggc ttagtgatct cgcctcaatc
      541 cccgttgtca gcgggttaca tcaccctgcg ggcatgccgc aggtcactgc cgctcaggtt
      601 ctccaccgtc acacccggtg ggattgttgg gtttctcatc gtgagttacc ggttcaatat
      661 tccagacaga ctcgcggttc atttaagcat ccatgcccgc cctgaactcc gggcacaccg
      721 taagtaaaat cagccaccca cagctggtca ggtcgttctg ccacgaactg acggtttacg
      781 cggtcgcctg cgg
//
LOCUS       DAJVUJ010000345          792 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.349, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000345 DAJVUJ010000000
VERSION     DAJVUJ010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..792
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.349"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..648
                     /locus_tag="OQQ48_004327"
     CDS             <1..648
                     /locus_tag="OQQ48_004327"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HCS1954839.1"
                     /translation="TYKYADMLAMSAKKDAMVPMGGLLCMKDDSFFDVYTECRTLCVV
                     QEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYRIAQVQYLVDGLEEIGVVCQQAGGH
                     AAFVDAGKLLPNIPADQFPAQALACELYKVAGIRAVEIGSFLLGRDPKTGKQLPCPAE
                     LLRLTIPRATYTQTHMDFIIEAFKHVKENAANIKGLTFTYEPKVLRHFTAKLKEV"
BASE COUNT          205 a          188 c          210 g          189 t
ORIGIN      
        1 acctacaaat atgccgatat gctggcgatg tccgccaaga aagatgcgat ggtgccgatg
       61 ggcggcttgc tgtgcatgaa agacgacagc ttctttgatg tgtacaccga gtgcagaacc
      121 ctttgcgtgg tgcaggaagg cttcccgaca tatggcggcc tggaaggcgg cgcgatggag
      181 cgtctggcgg taggtctgta tgacggcatg aatctcgact ggctggctta tcgtatcgcg
      241 caggtacagt atctggtcga tggtctggaa gagattggcg ttgtctgcca gcaggcgggc
      301 ggtcacgcgg cattcgttga tgccggtaaa ctgctgccga atatcccggc agaccagttc
      361 ccggcacagg cgctggcgtg cgagctgtat aaagtcgccg gtatccgtgc ggtagaaatt
      421 ggctctttcc tgttaggccg cgatccgaaa accggtaaac aactgccatg cccggctgaa
      481 ctgctgcgtt taaccattcc gcgcgcaaca tatactcaaa cacatatgga cttcattatt
      541 gaagccttta aacatgtgaa agagaacgcg gcgaatatta aaggattaac ctttacctac
      601 gaaccaaaag tattgcgtca cttcaccgca aaactgaaag aagtttaatt aatactacag
      661 agtggctata aggatgttag ccactctctt accctacatc ctcaataaca aaaatagcct
      721 tcctctaaag gtggcatcat gactgatcaa gctgaaaaaa agcactctgc attttggggt
      781 gttatggtta ta
//
LOCUS       DAJVUJ010000346          791 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.350, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000346 DAJVUJ010000000
VERSION     DAJVUJ010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.350"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..598
                     /locus_tag="OQQ48_004328"
     CDS             <1..598
                     /locus_tag="OQQ48_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA
                     acyltransferase"
                     /protein_id="HCS1954840.1"
                     /translation="GLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGKKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHAREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHLLTIEIRPPMDACIASADNKMI
                     ARQMNKTVEILVGPHPEQYVWVLKLLKTRKSNEADPYP"
BASE COUNT          249 a          156 c          183 g          203 t
ORIGIN      
        1 aggcctcgaa attcttgagg aaattcgaca caataataga aatgtgattt ttctggttcc
       61 ccatggctgg agcgtggata ttcctgcaat gttgctggca gctcagggaa aaaaaatggc
      121 cgccatgttt catcagcaac gaaatccagt gattgattat gtctggaatt cagtacggcg
      181 taaattcggg gggcgcttac atgcccggga ggatgggata aaaccattta ttcagtcagt
      241 acgccaggga tactggggat attacctccc agatcaggat catggtcctg aatacagtga
      301 atttgctgat ttttttgcga cctataaagc gacattacca atcattggac gtctgatgaa
      361 catcagtcag gctatgatta taccactttt cccggtttat gatgaaaaaa aacatctcct
      421 gactattgaa attcggccac caatggatgc ttgcattgcc agcgcggaca ataaaatgat
      481 tgcccgacaa atgaacaaaa cagtggaaat tttggtgggg ccacatccgg aacagtatgt
      541 ctgggtttta aaattgttaa aaacacgcaa atcaaacgaa gcggacccgt acccttgaaa
      601 ttataaagta taagtatttc cactagttgt ttgtaagcgt acagcctgaa ccgtctggtc
      661 agaatctgac gaattagaca aagtggtgtc caccaaataa gtagtgggaa ccaatgaggt
      721 agcctgagtt taacggacac tccttcctga aatagaatgg catcagaagg agctaataat
      781 gagcagaaaa a
//
LOCUS       DAJVUJ010000347          779 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.351, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000347 DAJVUJ010000000
VERSION     DAJVUJ010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..779
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.351"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            1..779
                     /locus_tag="OQQ48_004329"
                     /pseudo
     CDS             1..779
                     /locus_tag="OQQ48_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558238.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    243..359
                     /locus_tag="OQQ48_004329"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT          173 a          186 c          245 g          175 t
ORIGIN      
        1 agggcgattc gtatggttct ggaaagtcag ggcgaatatg actcacagtg ggcggcaatt
       61 tgttccattg ccccaaagat tggctgtaca ccggagactc tgcgtgtctg ggtacgccag
      121 catgagcggg ataccggagg cggtgatggc gggctcacca ccgctgaacg tcagcgtctg
      181 aaagagctgg aacgtgaaaa tcgtgaactg cgccgcagta acgatatcct tcgccaggct
      241 tccgcttatt ttgcgaaggc ggagttcgac cgcctctgga aaaagtgatg ccactgctgg
      301 ataagctgcg taagctgtac ggggtcggac cggtatgcag tgaactacat attgccccgt
      361 caacgtatta ccactgtcag caacagcgac atcatcctga taaacgcagt gcccgtgcgc
      421 agcgcgatga ctggctgaag aaagagatac tgcgcgtata cgatgggaat catcaggtat
      481 acggtgtgcg taaagtctgg cgtcagttgt tacgggaagg tatcagagtg gccagatgca
      541 ctgtggcacg tctcatggcg gttatgggac ttgccggtgt tctccggggt aaaaaggtcc
      601 gtacgaccat cagccggaaa gccgttgccg caggcgaccg cgtaaaccgt cagttcgtgg
      661 cagaacgacc tgaccagctg tgggtggctg attttactta cgtcagcaca tggcagggct
      721 tcgtctatgt ggcgttcatt attgatgtgt ttgccggata catcgtgggg tggcgggtc
//
LOCUS       DAJVUJ010000348          768 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.352, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000348 DAJVUJ010000000
VERSION     DAJVUJ010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..768
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.352"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            87..>768
                     /locus_tag="OQQ48_004330"
     CDS             87..>768
                     /locus_tag="OQQ48_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCS1954841.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFD"
BASE COUNT          261 a          188 c          109 g          210 t
ORIGIN      
        1 ctactgctga agggaaccaa taacattgca aagcagataa aaaagaatcc aacacctctc
       61 aacataaaga atacgccctg atatttatgc tcccgacaaa taacaatcac agattaattt
      121 caaattcgtt ctccacttat tcaatcgaca ctagccgcgc atatgaaaat tatctaaccc
      181 attggactga atggaaaaat aaccgcatac aagaagaaca acgagacatc gcttttcagc
      241 gactagtatc atgtctacaa aaccaagaga cgaacctaga cttgtctgaa ttaggcctga
      301 caacattacc tgaaatcccc ccgggaatta aatcaattaa tataagtaaa aataatttaa
      361 gcttaatctc cccattgcct gcgtccctta cacagcttaa tgtcagctat aacagactta
      421 ttgaactgcc tgctttgcct caaggactta aattattgaa tgcgtcccac aatcaactaa
      481 tcacactacc cacactcccc atatctttga aggagcttca tgtctcaaat aatcaattat
      541 gttctcttcc tgttttacca gaactactgg aaacattaga tgtatcatgt aatgggctgg
      601 cagttttacc acctttacca ttttctttac aagagattag cgcaataggg aatcttctta
      661 gtgaactccc ccctctacct cacaacattc actccatatg ggcaatcgac aatatgttaa
      721 ccgatattcc atacctgccg gaaaatttaa ggaacggtta ttttgaca
//
LOCUS       DAJVUJ010000349          726 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.354, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000349 DAJVUJ010000000
VERSION     DAJVUJ010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.354"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            35..726
                     /locus_tag="OQQ48_004331"
                     /pseudo
     CDS             35..726
                     /locus_tag="OQQ48_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          170 a          181 c          200 g          175 t
ORIGIN      
        1 atttagtgta tgatggtgat tttaaggtgc ttgcgtggct tccatttcca tcagatgtcc
       61 ttcctgctcc gctactgaag gcgtggtgcg taacggcaaa agcactgccg gacatcagcg
      121 ctatctctgc tctcattgcc gtaaaacatg gcaactacag ttcacttaca ccgcctctca
      181 gcccggtacg caccagaaaa tcattgatat ggccatgaat ggcgtcggat gtcgcgccag
      241 tgcacgcatt atgggcgttg gcctcaacac ggttttacgt cacttaaaaa actcaggccg
      301 cagtcggtaa cctcgcgcat acaaccgggc agtgatgtga ttgtctgcgc tgaaatggac
      361 gaacagtggg gctacgtcgg tgctaaatca cgtcagcgct ggctgtttta cgcgtatgac
      421 aggatacgga ggacggttgt ggcgcacgtc ttcggtgaac gcactctggc cacactggag
      481 cgtcttctga gcctgctgtc ggcctttgag gtcgtggtat ggatgacgga tggctggccg
      541 ctgtatgaat cacgcctgaa gggaaagctg cacgttatca gcaagcgtta cactcagcgc
      601 attgagcgac ataatctgaa tctgagacaa catctggcaa ggctgggacg gaagtcactg
      661 tcgttctcaa aatcggtgga gctgcatgac aaggtcatcg ggcattatct gaacataaaa
      721 cactat
//
LOCUS       DAJVUJ010000350          726 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.355, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000350 DAJVUJ010000000
VERSION     DAJVUJ010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.355"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..>726
                     /locus_tag="OQQ48_004332"
     CDS             <1..>726
                     /locus_tag="OQQ48_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS91-like element ISSbo1 family transposase"
                     /protein_id="HCS1954842.1"
                     /translation="HVRYVTNSCGSRACPSCGKKATDLWIATQLNRLPDCDWVHLVFT
                     LPDTLWPVFESNRWLLNDVCRLAVENLLYAARKRGQEPGIFCAIHTYGRRLNWHPHVH
                     VSVTCGGLNKHGQWKKLSFLKDAMRSRWMWNMRQLLLKAWSEGLAMPESLSHITTESQ
                     WRSLVLKAGGKYWHVYMSKKTAGGRNTARYLGRYLKKPPIAASRLAHYNGGASLNFRY
                     LDHKTGETATETLTQRELVARLK"
BASE COUNT          170 a          190 c          230 g          136 t
ORIGIN      
        1 ccacgtcaga tacgtcacta actcatgcgg cagccgtgcc tgcccgtcct gcggaaaaaa
       61 ggccacagac ctgtggatag cgacacagct gaatcgtctt cctgactgcg actgggtaca
      121 cctggtcttc accctgccgg acacgctgtg gccggtgttc gaaagcaacc ggtggctgct
      181 gaatgacgtg tgccgtctgg cggtggagaa tctgctgtat gccgcccgga aacgggggca
      241 ggaacccggt atcttctgcg ccatccacac gtatggccgt cgtctcaact ggcatccgca
      301 tgtacatgtg tctgtaacct gtggaggtct gaataagcat ggtcagtgga aaaagctgag
      361 cttcctgaaa gacgcgatgc gttcacggtg gatgtggaat atgcggcagc tgcttctgaa
      421 agcgtggtca gaggggctgg cgatgccgga gtcgttgtca catatcacga cggaatcaca
      481 gtggagaagc ctggtgctga aagccggagg aaaatactgg catgtgtaca tgtcgaaaaa
      541 aacggccggc gggaggaata cggcgcgcta cctgggtcgt tatctgaaga agccgccgat
      601 agcggcctcc cggctggcac attacaacgg aggggcgagc ctgaacttcc gttacctgga
      661 ccacaaaacg ggagaaacgg cgacggaaac gctgacacag cgtgagctgg tcgcgaggct
      721 gaaaca
//
LOCUS       DAJVUJ010000351          711 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.356, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000351 DAJVUJ010000000
VERSION     DAJVUJ010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..711
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.356"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(<1..>711)
                     /locus_tag="OQQ48_004333"
     CDS             complement(<1..>711)
                     /locus_tag="OQQ48_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HCS1954843.1"
                     /translation="DDVDVYSRDFSRTSQMNTNSVDVRYRNIPLWDDATLSLMAKYSA
                     PNKTDQQQDNENDNSYFEMKDSWMLTSVLRQNLQRDTFNEFTLQVANNSYASSFASFS
                     DASNTMAHGRYYYGDHTNGIAWRLISQGEMYLTDNIIMANALVYSHGEDVYSYESGAH
                     SDFDSIRTVIRPAWIWNTWNQTGLELGWFKQQNKTQQGVTLNESAYKTTLWHALKVGE
                     SILGSRPEIRFYGTYINIL"
BASE COUNT          210 a          156 c          134 g          211 t
ORIGIN      
        1 agaatattga tatacgttcc atagaagcga atttctggtc gtgaacctaa aatgctttca
       61 cccactttca atgcatgcca gagtgtcgtt ttataagccg attcatttag tgttacaccc
      121 tgctgagttt tgttctgttg cttaaaccag cctaattcaa gccccgtctg attccatgta
      181 ttccagatcc aggccggtct tattacagtg cgaatactgt caaaatcact atgagcacca
      241 ctttcataac tataaacatc ttcgccgtga gaatagacaa gcgcgttagc cataataata
      301 ttgtcagtaa gatacatctc gccctgagag attaaacgcc aggcgatccc attggtatga
      361 tcaccatagt aatagcgacc atgcgccatc gtattactgg catctgagaa actggcaaaa
      421 ctgctggcat aggaattatt ggcaacctgt aacgtaaatt cattaaacgt atcgcgttgc
      481 aggttttgcc gtaaaacaga agtcagcatc cagctatctt tcatttcaaa ataactgttg
      541 tcattttcgt tatcttgttg ttgatccgtt ttattaggtg cggaatattt agccattaat
      601 gataatgtcg catcatccca taacgggata ttgcgataac gaacatccac agaattggta
      661 ttcatctggc tggtacgcga aaaatcacga gagtaaacat cgacatcatc a
//
LOCUS       DAJVUJ010000352          697 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.357, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000352 DAJVUJ010000000
VERSION     DAJVUJ010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..697
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.357"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            1..697
                     /gene="istA"
                     /locus_tag="OQQ48_004334"
                     /pseudo
     CDS             1..697
                     /gene="istA"
                     /locus_tag="OQQ48_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS21 family transposase"
BASE COUNT          156 a          185 c          194 g          162 t
ORIGIN      
        1 tcagagacca gggatatcgt ggcggaatga ccattctcag ggcgttcatt cgttctctct
       61 cggtttctca ggagcaggag cctgccgttc ggttcgaaac tgaacccgga cgacagatgc
      121 aggttgactg gggcactatg cgtaatggtc gctcaccgct tcacgtgttc gttgctgttc
      181 ccggatacag ccgaatgttg tacatcgaat tcactgacaa tatgcgttat gacacgctgg
      241 agacctgcca tcgtaatgcg ttccgcttct ttggtggtgt gccgcgcgaa gtgttgtatg
      301 acaatatgaa aactgtggtt ctgcaacgtg acgcatatca gaccggtcag caccggttcc
      361 atccttcgct gtggcagttc ggcaaggaga tgggcttctc tccccgactg tgtcgcccct
      421 tcagggcaca gactaaaggt aaggtggaac ggatggaaca gtttttacat cccactaatg
      481 actcgcctgc gcccgatggg gagcactgtc gatgttgaaa cagccaaccg ccacggtctg
      541 cgctggctgc acgatgtcgc taaccaacga aagcatgaaa caatccaggc ccgtccctgt
      601 gatcgctggc tcgaagagca gcagtccatg ctggcactgc ctccggagaa aaaagagtat
      661 gacgtgcatc ctggtgaaaa tctggtgagc ttcgaca
//
LOCUS       DAJVUJ010000353          692 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.358, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000353 DAJVUJ010000000
VERSION     DAJVUJ010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.358"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..298
                     /gene="pocR"
                     /locus_tag="OQQ48_004335"
     CDS             <1..298
                     /gene="pocR"
                     /locus_tag="OQQ48_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="regulatory protein PocR"
                     /protein_id="HCS1954844.1"
                     /translation="SDDLRLEDVASHVYLSPYYFSKLFKKYHGIGFNAWVNQQRMASA
                     RELLCHSDWSIASIARNLGFSQTSYFCKVFRQTYQVTPQVYRQQVSENAAMETV"
     regulatory      387..562
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
BASE COUNT          198 a          146 c          183 g          165 t
ORIGIN      
        1 atcagacgat ttgcgtctgg aagatgtcgc ctcgcacgtt tatctaagcc cttattattt
       61 cagtaagtta ttcaagaaat accatggaat cgggtttaat gcgtgggtaa accaacaacg
      121 gatggccagc gcgcgtgagt tactgtgcca tagtgactgg agcatcgcca gcattgcgcg
      181 taatttaggt ttttcgcaaa ccagttattt ttgcaaagtt ttccgtcaga cttatcaggt
      241 tacgccgcag gtgtatcgac agcaagtgag tgaaaatgcg gcgatggaaa cggtatagag
      301 taaatgctaa aaaaagggaa ggtttaaata aacgctggaa tgaataatta acgttattta
      361 cgtgttatgg gaaaagtaaa atcagctcat aacatacgct gacaggtgtg gcacatttgt
      421 gcctgggaag agagtgaaaa tccctcgcag cccccgctgc tgtaatgctg acaaacccga
      481 aaatgccact gatcgcgaga ttgggaaggt cgggagagga tgacgctaag ccagaagacc
      541 agccagtcag tcaataccaa caacttcggt gggaagtggg ttgcccggaa gcgtccggta
      601 gagtacaggc gcgtctttat acccctacca ccctgaaagg atcagggtat ggcggcatca
      661 tttagttctt tcatcgcagt gccgacaggc ac
//
LOCUS       DAJVUJ010000354          691 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.359, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000354 DAJVUJ010000000
VERSION     DAJVUJ010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..691
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.359"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            74..>691
                     /locus_tag="OQQ48_004336"
     CDS             74..>691
                     /locus_tag="OQQ48_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCS1954845.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVP"
BASE COUNT          144 a          187 c          197 g          163 t
ORIGIN      
        1 ttcgtcaggc tcccttattc cgaacggcca ccgcgtccgt cctgcacatt ccagcaacgg
       61 atctggaggt aatgtgcggg tattacttcg acctgttctg gttccggaac tcgggctggt
      121 ggtccttaag ccaggccgtg aatccatgcc ggtattccac aatacccggg tactggtgga
      181 gccggaaccg aaaagcatgc gtaatctgcc gtccggggtc gttcctgccg ttcgccagcc
      241 gctagtggaa gacaaaacat tgctgccgtt tttcagtaac gcacgggtga ttcgtgctgc
      301 tggtggtgct ggtgcattgt ctgactggct gttgcgccat attaaatcct gccagtggcc
      361 acacggcgat tatcatcaca gcgaaaccgt cattcaccgt tatggtaccg gcgcaatggt
      421 gttgtgctgg cactgcgaca accagttgcg tgaccagaca tccgaatcac tcgagcaact
      481 tgctcatcaa aacctgtcag catggatgat tgacgtcatc ggtcacgcaa taagcggtac
      541 gcaggagcgt gaattatctc tggctgaatt atcctggtgg gcggtccgca atcaggtggc
      601 ggacgcgcta ccggaagcag tattacgtcg ttcgctgggg ttgcgtgcgg aaaaaatccg
      661 ctcaatgtac cgtgaaagcg acatcgtgcc g
//
LOCUS       DAJVUJ010000355          689 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.360, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000355 DAJVUJ010000000
VERSION     DAJVUJ010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..689
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.360"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(116..388)
                     /locus_tag="OQQ48_004337"
     CDS             complement(116..388)
                     /locus_tag="OQQ48_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HCS1954846.1"
                     /translation="MVHDRIAEELEAKGFYRRAAARWGEVMLLVETDKERHQVTMRRL
                     ECSRKAQKPPEPPDNFGDLRKAVDRTYAEMGIDGVSDEIWRNYPDS"
     gene            complement(399..>689)
                     /locus_tag="OQQ48_004338"
     CDS             complement(399..>689)
                     /locus_tag="OQQ48_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCS1954847.1"
                     /translation="QDGQATLWLSVIAFGKQADFLAKHQKGDVASVSGTMQVSQWTGQ
                     NGETRQGYQVIADSVISVRAARPGGNRRKTTGTQGNQPPAGGDDPYGDGIPF"
BASE COUNT          125 a          220 c          158 g          186 t
ORIGIN      
        1 ctctgttgtg tctgtttgtt tcagatagta actatgcctg accgtgacga aaacccggta
       61 atgcgccata ccgtttcaac tggtgcaaaa aaagccggat ttctccggct gttgattagc
      121 tgtctgggta attacgccat atttcatcgc ttacaccatc tatacccatt tcagcataag
      181 tgcgatctac tgcctttctc aggtctccga agttatccgg cggctccggt ggcttctgtg
      241 ccttcctgga acattccagc cgtcgcatcg taacctgatg ccgttccttg tctgtctcca
      301 ccagcagcat gacttcaccc catcgcgccg ccgccctccg gtaaaagcct ttcgcctcga
      361 gttcttccgc tatgcggtca tgtaccatcg ccaccccctc agaacggaat accgtcaccg
      421 taagggtcat cgcctcccgc tggtggctga ttaccctgtg tgcctgtggt tttgcgtctg
      481 ttcccgccag gacgtgccgc acggacactg attacgctgt ctgcaataac ctgataaccc
      541 tgccgcgttt ccccgttctg tccggtccac tggctgacct gcatcgtgcc ggatacgctg
      601 gcaacgtcgc ctttttgatg tttagccagg aagtcggcct gcttaccaaa tgcgatgacc
      661 gatagccata acgtcgcctg cccatcctg
//
LOCUS       DAJVUJ010000356          673 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.361, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000356 DAJVUJ010000000
VERSION     DAJVUJ010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..673
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.361"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            193..462
                     /locus_tag="OQQ48_004339"
     CDS             193..462
                     /locus_tag="OQQ48_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1954848.1"
                     /translation="MAQVNMSVRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQ
                     HNSWFRDQVAAGRQQLECGDVLPHDMVESSAAAWRDEMSRKIADK"
     gene            459..673
                     /locus_tag="OQQ48_004340"
                     /pseudo
     CDS             459..673
                     /locus_tag="OQQ48_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585073.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
BASE COUNT          168 a          154 c          197 g          154 t
ORIGIN      
        1 ctgtcagcgc cagtgatagg acaggtgaaa cctgtcaata cgtaccggct tacgccgggc
       61 ttcggcggtt ttactcctct gccatatgca acaacggagt gccgccttac atgccgctgg
      121 cgcggcatat cctggtgacg atatctgaac agttatatac tgtgtatata cgtggtaatg
      181 acgaggtggc aaatggcaca ggttaatatg agtgtaagaa tcgacgcaga actgaaggat
      241 gcttttatgg ccgctgcaaa aagcatggac cgtaatggct ctcagttaat ccgggatttt
      301 atgcgccaga ccgttgaacg gcagcacaat tcttggttcc gtgaccaggt tgcggcaggc
      361 cgtcagcaac tcgaatgcgg tgatgtgctt ccccatgaca tggtcgaatc ctctgctgct
      421 gcatggcggg atgaaatgag caggaagatt gctgataaat gatggagatg ttctggacca
      481 tgctggccat ccaggacagg aagcgcattc gtagtacatt gcggagcaga acctgatggc
      541 cgccattgaa ccggacgaac ggattggtta ttcagccagc agcctggcag ggcagccgta
      601 taaaggccgc aacggtcgtg tggaaggaac atctggacca cacaaggtag cgtgtaacgt
      661 tattttgtgt gaa
//
LOCUS       DAJVUJ010000357          661 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.362, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000357 DAJVUJ010000000
VERSION     DAJVUJ010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..661
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.362"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(<1..572)
                     /locus_tag="OQQ48_004341"
     CDS             complement(<1..572)
                     /locus_tag="OQQ48_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005044736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1954849.1"
                     /translation="MVGFGNKYIAAQFGISPGLAATVNASVLIAALIGGLLANRVINR
                     FGQKRAFIIGMGLCTIGAAAVAIAPSIWWVLVCRVIMGFGLGIDFPLATNAVAELRGS
                     TSKKTGTSVNLWQMAWYVSTTVVYLVLLPLLLSGIAEEQLWRYGIFIGAIFAVIFMIL
                     RYFFIGESAMWAARVGRYQEACDILGKRYGV"
BASE COUNT          201 a          182 c          142 g          136 t
ORIGIN      
        1 acaccataac gttttcccag aatgtcgcac gcttcctggt aacgcccgac gcgtgcggcc
       61 cacattgcgg attcaccaat aaagaagtaa cgcaaaatca tgaagatgac tgcaaaaata
      121 gctccgatga atattccgta acgccacaat tgttcttcag cgatacccga cagaagcagc
      181 ggcaaaagta ccaaataaac aacggttgtc gaaacatacc aggccatttg ccagaggttg
      241 accgacgttc cggttttctt cgacgttgaa ccacgaagct ctgccacggc attggttgcc
      301 aaagggaagt cgatgcctaa accaaagccc atgatgacgc ggcacaccag cacccaccag
      361 atactgggag caatagctac cgctgcagca ccgatggtgc acagccccat gccaataata
      421 aatgcccgct tttgcccaaa gcggtttatt actcggttcg ctaataaacc gccaattaac
      481 gcggcgatta atactgatgc attgacggtt gcagcaagac ccggcgaaat gccgaattga
      541 gcggcaatgt atttattacc aaaccccacc atcgccgcct agtaggcatc aatcaatatg
      601 ccgcccaaag caataaaaat aacgccgata ctggtgttga ttgcagagtt cttattaaca
      661 a
//
LOCUS       DAJVUJ010000358          658 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.363, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000358 DAJVUJ010000000
VERSION     DAJVUJ010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..658
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.363"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..456
                     /locus_tag="OQQ48_004342"
     CDS             <1..456
                     /locus_tag="OQQ48_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1954850.1"
                     /translation="NIQAGTARKFTLSSANNSTKNAAFYLWGNPSRSVVAELGDDSGW
                     HFFSQRNTDNSITFAVNGQVIPLNYGNFDARYKYRTEGVQDVRYGHEMYYSPGSNTVS
                     WRFRAPSGHGLSGIAISDTGRDSADNVNGVYYRPLQKLINGTWYNVASV"
     gene            459..>658
                     /locus_tag="OQQ48_004343"
     CDS             459..>658
                     /locus_tag="OQQ48_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCS1954851.1"
                     /translation="MLHLKNITAGNPKTVEQYQLTKQYDVTWLFSKDGKNWYEELKNF
                     ASDTIKITYTGDGRVVWVGKDVT"
BASE COUNT          194 a          142 c          168 g          154 t
ORIGIN      
        1 aatattcagg ccggaactgc cagaaaattc acgttatcca gcgccaacaa ctccacgaaa
       61 aatgcagcgt tttatttgtg gggtaatcca tccaggtctg ttgttgcaga gcttggcgat
      121 gattcaggct ggcatttttt cagccagagg aatacagata acagcatcac gttcgccgta
      181 aacggacagg taattccgtt aaattacgga aacttcgatg cccgctataa atatcgaaca
      241 gagggggtac aggatgtacg gtatggccat gaaatgtatt acagccccgg cagtaacacc
      301 gtttcgtgga gatttcgcgc accttcggga cacgggctgt cagggatagc gatatcggat
      361 accggccgtg actcagcgga taacgtcaac ggcgtgtatt accgcccgct tcagaaactg
      421 attaatggca cctggtataa cgtggcgagt gtttaacaat gttgcattta aaaaatatta
      481 ctgcgggcaa tccgaaaacc gtggaacaat atcagctgac aaaacaatat gatgtcacct
      541 ggcttttttc gaaagacgga aaaaactggt atgaagagct gaagaatttc gccagcgaca
      601 caattaaaat aacttacacc ggagacggtc gcgtggtgtg ggtcggtaag gatgtgac
//
LOCUS       DAJVUJ010000359          658 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.364, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000359 DAJVUJ010000000
VERSION     DAJVUJ010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..658
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.364"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(17..289)
                     /locus_tag="OQQ48_004344"
     CDS             complement(17..289)
                     /locus_tag="OQQ48_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HCS1954852.1"
                     /translation="MKTSDNERIKYEITGQVVLQILRMKINFSLQTLIKQLLVMKSAE
                     EDAFRRDLIDSIIRDFSNSDSGGPNRRTATADNKSMFNGKKINRIH"
     gene            complement(332..568)
                     /locus_tag="OQQ48_004345"
     CDS             complement(332..568)
                     /locus_tag="OQQ48_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YcgZ"
                     /protein_id="HCS1954853.1"
                     /translation="MHQNSVTLDSAGAITRYFAKANWPTQQETLGEIVTEILKDGRNL
                     SRKSLCAKLLCRLEHATGEEEQKHYNALIGLLFE"
BASE COUNT          190 a          136 c          111 g          221 t
ORIGIN      
        1 tcttacttat gaaattttaa tgtattctgt ttattttctt accattgaac atacttttat
       61 tgtcagctgt cgctgttctt cggtttggcc ctcctgaatc actattgcta aaatcacgaa
      121 ttatgctgtc aattaaatcg cgtcggaaag catcttcctc agccgatttc attacgagta
      181 attgcttaat aagagtctgc aatgaaaaat ttatcttcat gcgcagtatc tggagcacca
      241 cctggccagt aatttcatat tttatacgtt cattatcaga tgtcttcata acgacttcat
      301 agctaacctg ttaaataatc aggctgttgc attattcaaa aagcaaccca attagtgcat
      361 tataatgttt ctgttcctct tccccagtcg cgtgttccag tcggcaaaga agtttggcac
      421 aaagcgactt tcgactcaga ttacgtccat ctttcaaaat ctcagtcaca atttccccga
      481 gagtttcctg ctgagtaggc cagttagctt ttgcaaagta acgagtgatt gctcccgcag
      541 aatctaaagt cactgaattt tgatgcatgc tacgcctctg ttaaaaatgt taatcaatgt
      601 taagtgaggt aacaatagca aacttgtacg aaatatgtgt acagaatgat aaattaat
//
LOCUS       DAJVUJ010000360          638 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.365, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000360 DAJVUJ010000000
VERSION     DAJVUJ010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..638
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.365"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..>638
                     /locus_tag="OQQ48_004346"
     CDS             <1..>638
                     /locus_tag="OQQ48_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308607.1"
                     /note="multicopy suppressor of dominant negative ftsH
                     mutations; putative acyl-CoA synthetase; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HCS1954854.1"
                     /translation="MGTPANKALLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQA
                     IMQQLEEALKQLAQGAGSSQSLTQVRRWDSACQKLSDANLALISVAGEYAAELANQAL
                     ERNLNVMMFSDNVTLEDEIQLKTRAREKGLLVMGPDCGTSMIAGTPLAFANVMPEGNI
                     GVIGASGTGIQELCSQIAQAGEGITHAIGLGERDLSREVGGISALTALEMLS"
BASE COUNT          143 a          155 c          212 g          128 t
ORIGIN      
        1 gatgggtacg cccgccaata aagcgttatt agataccaca ggtttctggc atgacgattt
       61 taataacgcc acgccgaacg atatttgcgt ggcaattcgt agcgaagcgg cggatgcggg
      121 gatcgcgcag gcgattatgc agcagcttga agaggcgcta aaacaactgg cgcagggggc
      181 aggcagcagc cagtccttga cgcaggtgcg tcgctgggac agtgcctgtc agaaattatc
      241 cgatgccaat ctggcgctga tttcagtggc tggcgagtat gcggcggagc tggcgaatca
      301 ggcgctggag cgcaacctca acgtgatgat gttctccgat aacgtcacgc tggaagatga
      361 aatccaactt aaaacccgcg cgcgagaaaa aggcttgctg gtgatggggc cggactgcgg
      421 tacgtcgatg attgccggca cgccgctggc ttttgctaac gtgatgccgg aaggcaacat
      481 tggcgtcatt ggcgcttccg gtaccgggat tcaggaactg tgttcgcaga ttgcgcaggc
      541 aggggaggga attactcacg cgattggtct tggcgagcgc gacctcagcc gtgaagtagg
      601 cggcatcagt gcgctgacgg cgctggaaat gctcagtg
//
LOCUS       DAJVUJ010000361          632 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.366, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000361 DAJVUJ010000000
VERSION     DAJVUJ010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..632
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.366"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..>632
                     /gene="pdeB"
                     /locus_tag="OQQ48_004347"
     CDS             <1..>632
                     /gene="pdeB"
                     /locus_tag="OQQ48_004347"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HCS1954855.1"
                     /translation="LSIWLAHQQVETSFIEELDTYSSRVAIRANKVATQGKDALQELE
                     RWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNVPQCSSLEHESPPDTFPEPGKISK
                     DGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPASFIDVIPYSSWQIDAAIIGNAHNV
                     VITSSDEIAQGIITRLQKTPGEHIENNGIIYDILPFPEMNISIITWASTK"
BASE COUNT          173 a          152 c          145 g          162 t
ORIGIN      
        1 cttaagcatc tggctggccc atcagcaggt agaaacatcg tttattgaag agctggatac
       61 ctattcctcc cgcgtcgcta ttcgggccaa taaggtggcg acacaaggga aagatgcgct
      121 gcaagagctg gaaagatggc aaggcgctgc ctgtagcgaa gcccatctca tggaaatgcg
      181 tcgggtatct tacagttatc gctatattca ggaagtggtt tatatcgata acaacgttcc
      241 ccagtgttcg tctctggagc atgaaagtcc gcccgatacc ttccccgagc caggtaaaat
      301 ttcgaaagat ggttatcgtg tctggttaac atcgcataac gatttaggca ttatccgtta
      361 catggtcgcc atgggaacgg cacattatgt cgtcatgatc gaccccgctt cctttattga
      421 tgtcattccc tatagctcat ggcaaattga tgccgccatt attggcaatg cccataacgt
      481 tgtcataacc agcagcgatg aaattgctca gggaattatt accaggctac aaaaaacacc
      541 cggtgagcat atcgaaaata atggaatcat ttacgatatc ctgcccttcc cggagatgaa
      601 tatttcgatc atcacctggg cttcaacgaa aa
//
LOCUS       DAJVUJ010000362          631 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.367, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000362 DAJVUJ010000000
VERSION     DAJVUJ010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..631
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.367"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            106..>631
                     /locus_tag="OQQ48_004348"
     CDS             106..>631
                     /locus_tag="OQQ48_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630-like element IS630 family transposase"
                     /protein_id="HCS1954856.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCARSSVGRWINWFTQSGVEGLKSLPAGRARRWPFEHICTLLRELVKHSPGD
                     FGYQRSRWSTELLAIKINEITGCQLNAGTVRRWLPSAGIVWRRAAPTLRIRDPHKDEK
                     MAAIHKALDECSAEH"
BASE COUNT          162 a          170 c          167 g          132 t
ORIGIN      
        1 cctaaatagc tgcgcggaat agtagatccc tttgagggaa cttagcccgg attgtgcgat
       61 ctgatcaatc gccaaatcaa aacaaatcac caaccggact gagcaatgcc gatcatagca
      121 ccaatttccc gtgacgaacg acgcctgatg cagaaagcca tccataaaac acacgataaa
      181 aattatgccc gcagactgac tgccatgctg atgctgcacc ggggcgaccg tgtcagcgac
      241 gttgccagaa cgctctgctg cgcccgttcc tctgttggac gctggattaa ctggttcacg
      301 cagtcgggtg ttgagggact gaaatcatta cctgccgggc gtgcccgtcg ctggccgttt
      361 gagcatatct gcacactgtt acgtgagctg gtaaaacatt ctcccggcga ctttggctac
      421 cagcgttcac gctggagtac agaactgctg gcaataaaaa tcaatgagat aaccggttgc
      481 cagttaaatg ccggaaccgt tcgccgctgg ttgccgtctg cggggattgt gtggcgaagg
      541 gctgcgccaa ctctgcgtat ccgtgacccg cataaagatg aaaagatggc agcaatccat
      601 aaagcactgg acgaatgcag cgcagagcat c
//
LOCUS       DAJVUJ010000363          593 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.368, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000363 DAJVUJ010000000
VERSION     DAJVUJ010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.368"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            244..>593
                     /locus_tag="OQQ48_004349"
     CDS             244..>593
                     /locus_tag="OQQ48_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCS1954857.1"
                     /translation="MSKKLGFALSGLMLAMVAGTASADMDGGQLNISGLVVDNTCETR
                     VDGGNKDGLILLQTATVGEIDAGVLNDTVGAKAKPFSITVDCSKANPNPGSTAKMTFG
                     SVFFGNSKGTLNND"
BASE COUNT          171 a          111 c          122 g          189 t
ORIGIN      
        1 atcgccatac cctaaccaat tgcacttgtg aatttatttt tgcttaattt tgaattaaga
       61 aaatttaatt tgttcaaaag cccttaaagt gcacaatagg cgtcctaatg aacaatggtt
      121 gtaaattttg gtaacaaata tcattgctca ttaatatcta gcatctgctt ataacatttg
      181 tgtcatgaat agtcattctt caaaccagta gtctgactaa tggaatttta ttcaaggatt
      241 tttatgtcta aaaaattagg ttttgccctg tctggtctta tgctggcaat ggttgcaggt
      301 actgcttctg ctgatatgga tggtggtcag ttaaatatca gtggtctggt tgttgataac
      361 acctgtgaaa cccgcgttga cggcggtaat aaagatggtc tgatcctgct gcaaactgca
      421 actgttggtg aaatcgatgc tggcgtgctg aatgacaccg ttggcgctaa agccaaacct
      481 ttcagcatca ccgttgactg cagcaaagca aatccgaatc caggtagtac agctaaaatg
      541 accttcggtt ctgttttctt tggtaatagc aaaggaacgc tgaacaacga cat
//
LOCUS       DAJVUJ010000364          584 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.369, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000364 DAJVUJ010000000
VERSION     DAJVUJ010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..584
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.369"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            52..584
                     /locus_tag="OQQ48_004350"
                     /pseudo
     CDS             52..584
                     /locus_tag="OQQ48_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          187 a          142 c          144 g          111 t
ORIGIN      
        1 gccacctgaa cagaggtgat atgctcacct cagaacaaca caggtgctcc aatgaaaaaa
       61 agaaatttta gcgcagagtt taaacgcgaa tccgctcaac tggttgttga ccagaaatac
      121 acggtggcag atgccgccaa agctatggat gttggccttt ccacaatgac aagatgggtc
      181 aaacaactgc gtgatgagcg tcagggcaaa acaccaaaag cctctccgat aacaccagaa
      241 caaatcgaaa tacgtaagct gaggaaaaag ctacaacgca ttgaaatgga gaatgaaata
      301 ttaaaaaagg ctaccgcgct cttgatgtca gactccctga acagttctcg ataatcggga
      361 aactcagagc gcattatcct gtggtcacac tctgccaagt gttcggggtt catcgcagca
      421 gctacagata ctggaaaaac cgtcctgaaa aaccagacgg cagacgggct gtattacgca
      481 gtcaggtact tgagctacat ggcatcagcc atggttcggc cggagcaaga agcatcgcca
      541 caatggcaac ccggagaggc taccagatgg gacgctggct tgct
//
LOCUS       DAJVUJ010000365          583 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.370, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000365 DAJVUJ010000000
VERSION     DAJVUJ010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..583
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.370"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
BASE COUNT          168 a          119 c          116 g          180 t
ORIGIN      
        1 catacaacga tatttatctt cacgttagtg aagaataagt tgttttgtga tctatgttaa
       61 tctgggatta caatccgggc ttgatacatc gcatcacgac ttttattctc ttcagaatag
      121 aatatgttac tccccgtttt acaggctcag gatttgaatt acaaattata cttttaagtg
      181 aacaataact aggggtattt cttatttcct gaaaccaggc ttggcccatt tcgggatgag
      241 tttgaaaaaa ctcttgacgc atacgatagt gatgaaataa acgaaacaaa ttacgtcaac
      301 aaactcaggc gtcttgcctg acatgctcct gacatcattg atattcacgc tcatctggca
      361 tatgcatttg cgctctttat gctgcaattc tggcgaatgt tcatttacag cgtcatcagg
      421 atgccgttat actcattgat aaaatactaa catataaccc tgagaataat caggggacac
      481 gctggttact ggggacggaa ttgttacgtg ctggcaagca tgacagggct ctgtacgtat
      541 tgacaggaca tgtcaatgaa tttcccccct actggtacga gct
//
LOCUS       DAJVUJ010000366          578 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.371, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000366 DAJVUJ010000000
VERSION     DAJVUJ010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.371"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..>578
                     /gene="pdeB"
                     /locus_tag="OQQ48_004351"
     CDS             <1..>578
                     /gene="pdeB"
                     /locus_tag="OQQ48_004351"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HCS1954858.1"
                     /translation="PLAQQTGLSEPLTLLIIRSVFEDMGDWLRQHPQQHISINLESTV
                     LTSEKIPQLLREMINHYQVNPRQIALELTEREFADPKTSAPIISRYREAGHEIYLDDF
                     GTGYSSLSYLQDLDVDILKIDKSFVDALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQ
                     EEWLRQHGVHYGQGWLYSKALPKEDFLRWAEQ"
BASE COUNT          170 a          135 c          138 g          135 t
ORIGIN      
        1 tccgctggca cagcaaacag gcctttctga gccattgacg ctactgatta taagaagcgt
       61 ctttgaagat atgggcgact ggctgcgtca gcatccacag cagcatattt cgatcaatct
      121 tgaatccacc gtgctcacct cggaaaaaat cccgcaattg ctgcgtgaaa tgatcaatca
      181 ctatcaggtt aatcccagac agatcgcgct tgaactcact gaacgcgagt ttgccgatcc
      241 gaaaaccagc gccccgataa tttctcgcta ccgggaggcg ggccatgaaa tttatcttga
      301 tgattttggt acgggatatt caagtttaag ttatttacag gatctggatg tcgacattct
      361 gaagatcgat aaatctttcg ttgatgcgct ggaatataaa aatgtcacgc cgcatatcat
      421 cgaaatggca aaaacactga aactgaaaat ggtagcggag ggaatcgaaa ccagtaaaca
      481 agaagagtgg ctacgccagc atggcgtgca ctacggtcag ggctggctct acagcaaggc
      541 attaccgaaa gaagatttct tacgctgggc cgagcaac
//
LOCUS       DAJVUJ010000367          573 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.372, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000367 DAJVUJ010000000
VERSION     DAJVUJ010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.372"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(<1..153)
                     /locus_tag="OQQ48_004352"
     CDS             complement(<1..153)
                     /locus_tag="OQQ48_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1954859.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALD"
BASE COUNT          179 a          124 c          119 g          151 t
ORIGIN      
        1 atccagtgcc agttgagcaa aggttcggag ctgattattc acttctgatt ttgcccaaag
       61 gaaggcaatc catagcgaaa gaatgatggg aaagagaact aaaaagataa tgcccggcaa
      121 cgccagtaat tggtgtcgtg cacgatgact cattgctatc aatttcctgc ctgtttatac
      181 ccggccattg taaggtgaaa accctgggta ccgacgtctt tagataaatc ttacggtata
      241 aatagcactt ttgcctgatt tgaaattgat acctttctaa tcgtcacctg gatgaaaata
      301 attcattgat attcaaaatt aatttcctac aggaatgcag aggcggcggg aagatatgcc
      361 cttaatgcca ctgaaaaacc acataatctg tacagcaata aaagtcacgg cctaatatgg
      421 ctaacaaaca atcaattgtg atatatttca caaaattaat gaaacaaaca gagtgtttca
      481 tttttgtgtt ctgcaagccc cgcgatagcc agagtgtacc acgcctcccg tgaacaacgc
      541 cgcgctgtcc agggcatcgg ctcttttcag agg
//
LOCUS       DAJVUJ010000368          569 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.373, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000368 DAJVUJ010000000
VERSION     DAJVUJ010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..569
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.373"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..435
                     /locus_tag="OQQ48_004353"
     CDS             <1..435
                     /locus_tag="OQQ48_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1954860.1"
                     /translation="INEEALLIKNNLSDVEDRDEAVENLGLKPTVDKAKNAVQRDGDT
                     MTGELKIRGVNALRIFNEAFGLIFRRSEECLHLIPTSEGQGENGDIGPLRPFTINLRT
                     GEISMSHKVSVGGGSQVNGALGIGVQNALGGIQLLSGITIQV"
     gene            456..>569
                     /locus_tag="OQQ48_004354"
     CDS             456..>569
                     /locus_tag="OQQ48_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCS1954861.1"
                     /translation="MDVYANGQHVFRFQNGVAIALKNIQAGNAKKFTLSSAN"
BASE COUNT          156 a          114 c          160 g          139 t
ORIGIN      
        1 attaacgaag aggcattgct gataaaaaat aacctttcgg atgtggaaga cagggatgag
       61 gctgttgaaa acctcggatt aaaacccacg gtggataagg caaaaaatgc cgttcagcgt
      121 gatggcgaca ccatgaccgg agagctgaaa atccgtggtg ttaatgcgct gaggattttc
      181 aacgaagctt ttggcctgat ttttcgtcgt tcggaagagt gcctgcacct tattcccacc
      241 agtgaaggtc agggggaaaa tggcgatatt ggtcccctgc gcccgttcac cattaatctg
      301 cggacgggtg aaatatccat gtcgcataaa gtgtctgttg gcggtggttc gcaggtcaat
      361 ggtgcgctgg gtatcggcgt tcagaacgcc ctggggggaa ttcaattgct ttcggggata
      421 acgatacagg tataaaacaa aacggcgacg gcattctgga tgtttatgcg aatggacagc
      481 atgtatttcg tttccagaat ggtgtggcga tagcgttaaa aaatattcag gccggaaatg
      541 ctaaaaaatt cacgttatcc agcgccaac
//
LOCUS       DAJVUJ010000369          565 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.375, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000369 DAJVUJ010000000
VERSION     DAJVUJ010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..565
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.375"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..>565
                     /gene="ipaH9.8"
                     /locus_tag="OQQ48_004355"
     CDS             <1..>565
                     /gene="ipaH9.8"
                     /locus_tag="OQQ48_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HCS1954862.1"
                     /translation="WFPENKQSDVSQIWHAFEHEEHANTFSAFLDRLSDTVSARNTSG
                     FREQVAAWLEKLSASAELRQQSFAVAADATESCEDRVALTWNNLRKTLLVHQASEGLF
                     DNDTGALLSLGREMFRLEILEDIARDKVRTLHFVDEIEVYLAFQTMLAEKLQLSTAVK
                     EMRFYGVSGVTANDLRTAEAMVRSREE"
BASE COUNT          136 a          157 c          142 g          130 t
ORIGIN      
        1 catggttccc ggaaaacaaa caatctgatg tatcacagat atggcatgct tttgaacatg
       61 aagagcacgc caacaccttt tccgcgttcc ttgaccgcct ttccgatacc gtctctgcac
      121 gcaatacctc cggattccgt gaacaggtcg ctgcatggct ggaaaaactc agtgcctctg
      181 cggagcttcg acagcagtct ttcgctgttg ctgctgatgc cactgagagc tgtgaggacc
      241 gtgtcgcgct cacatggaac aatctccgga aaaccctcct ggtccatcag gcatcagaag
      301 gccttttcga taatgatacc ggcgctctgc tctccctggg cagggaaatg ttccgcctcg
      361 aaattctgga ggacattgcc cgggataaag tcagaactct ccattttgtg gatgagatag
      421 aagtctacct ggccttccag accatgctcg cagagaaact tcagctctcc actgccgtga
      481 aggaaatgcg tttctatggc gtgtcgggag tgacagcaaa tgacctccgc actgccgaag
      541 ccatggtcag aagccgtgaa gagaa
//
LOCUS       DAJVUJ010000370          550 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.376, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000370 DAJVUJ010000000
VERSION     DAJVUJ010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.376"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..>550
                     /locus_tag="OQQ48_004356"
     CDS             <1..>550
                     /locus_tag="OQQ48_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1954863.1"
                     /translation="QLSRPSEINRLKALVAKLQRMQFGKSSEKLRAKTERRIQEAQER
                     ISALQEEMAETLGEQYDPVLPSSLRQSSARKPLPASLPRAPRVIRPEEECCPACGGEL
                     SPLGCDVSEQLELISSAFKVIEKQRPKLACRRCDHIVQAPVPSKPIARSYAGAGLLAH
                     VVTGKYADHLPLYRQSDLLFHTA"
BASE COUNT          137 a          159 c          146 g          108 t
ORIGIN      
        1 cagttgtcca ggccttccga aatcaaccga ctgaaggcgc tggtcgcgaa actgcagcgt
       61 atgcagttcg gtaaaagctc agaaaaactt cgcgcaaaaa ccgaacggcg gatacaggaa
      121 gcacaggagc gaatcagcgc acttcaggag gaaatggcgg aaacactggg tgagcaatat
      181 gacccggtac tgccatcttc cctgcgccag tcttcagccc gtaaaccgtt accagcctca
      241 cttccccgtg ccccccgggt catccggccg gaagaggaat gctgtccggc ctgtggtggt
      301 gaactcagtc cactgggatg tgatgtgtca gagcagctgg agcttatcag cagcgccttt
      361 aaggttatcg aaaaacaacg ccctaaactg gcctgtcgcc ggtgcgacca tatcgtgcag
      421 gcaccggtgc cttcaaaacc cattgcacgc agttatgccg gagcggggct tctggcccat
      481 gttgttaccg gaaagtatgc agaccatctg ccattatacc gccaaagtga tctactattc
      541 cacacagcta
//
LOCUS       DAJVUJ010000371          547 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.377, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000371 DAJVUJ010000000
VERSION     DAJVUJ010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..547
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.377"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            167..503
                     /locus_tag="OQQ48_004357"
                     /pseudo
     CDS             167..503
                     /locus_tag="OQQ48_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          184 a           93 c          111 g          159 t
ORIGIN      
        1 ttccaattaa agccgttatt ataataatta tcatatatgg ttcttttcat gttgatttcg
       61 tcagaaacag aaaatgctga taatgaattg tgaataatca aactgacctc taattatcct
      121 gtatggagta gtgcttattt tcgactcaat ttctactgat tagattatgg ttatcagtac
      181 agtgggttat gaaaaacgga ctggatctgc cagcagtgct tctattatta ttcaaaaaga
      241 acttcagccg gacaatgcta taatccaggg aaaggctata agctctgttg gacggaatgt
      301 ggaaatcagc actggtaaaa aggggaactg acattaacac aagaaggatt cctgcaagca
      361 actgcttatc tgtccggaac gcagaattaa agcatgacca cacaggagcc agaatactgt
      421 ggtcgtacgc attcagtatt gtgacaacta atagaaataa aaatctggcc ggtgcgaata
      481 ttaaacgatg cgataccgta tagtgtaagt caaaagtaac ggggtattta aaccatcttt
      541 tcatatt
//
LOCUS       DAJVUJ010000372          536 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.378, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000372 DAJVUJ010000000
VERSION     DAJVUJ010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..536
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.378"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(<1..>536)
                     /gene="capU"
                     /locus_tag="OQQ48_004358"
     CDS             complement(<1..>536)
                     /gene="capU"
                     /locus_tag="OQQ48_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HCS1954864.1"
                     /translation="SPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIAFEASKFGI
                     DITFALFRNSLHIPTVWRLLGIVHSFQPDAIVCHSGHDSNIVGLVRFFTWKHPFRIIR
                     QKTYLTRKTKFFSINHFCDEVIVPGTNMKTHLEQEGCRTRVTVVPPGFDFQELYVDSR
                     NSLPPSVLSWLASRRGCPV"
BASE COUNT          163 a          123 c          102 g          148 t
ORIGIN      
        1 acggggcatc ctcttcggga tgccagccag gaaagaacac tcggaggcaa actgtttcgg
       61 gaatcaacgt ataattcctg gaaatcaaag ccaggaggca caacagtaac ccgagttcga
      121 catccttcct gctccagatg tgtcttcata tttgttccgg gaacaatcac ctcatcgcaa
      181 aaatgattta ttgaaaaaaa ttttgttttt cgtgtcaaat atgtcttttg cctgataatt
      241 ctgaatggat gtttccaggt aaaaaaccgt accaaaccaa caatattact atcatgtcca
      301 ctgtgacaaa cgattgcatc gggctgaaaa ctatgaacta ttccgagtaa tctccataca
      361 gtaggaatgt gaagactgtt tcgaaataac gcgaatgtga tatcaattcc aaacttactg
      421 gcttcaaaag caattttgct gttctccctg cagacaagca gaactgaatg ccccattttc
      481 ttcagggcct tcatttgagc aacagcctgt aattcctgac cacctatatt cggtga
//
LOCUS       DAJVUJ010000373          536 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.379, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000373 DAJVUJ010000000
VERSION     DAJVUJ010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..536
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.379"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..>536
                     /gene="capU"
                     /locus_tag="OQQ48_004359"
     CDS             <1..>536
                     /gene="capU"
                     /locus_tag="OQQ48_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HCS1954865.1"
                     /translation="SPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIAFEASKLGI
                     DITFALFRNSLHIPTAWRLLGIVHGFQPNAIVCHSGHDSNIVGLVRLFTWKHPFRIIR
                     QKTYLTQKTKVFSINHFCDEVIVPGTSMKTHLEQEGCRTRVTVVPPGFDFQKLYVDSR
                     NSLPPNVLSWLASRRGCPV"
BASE COUNT          153 a          105 c          122 g          156 t
ORIGIN      
        1 tcaccgaata taggtggtca ggaattacag gctgttgctc aaatgaaggc cctgaagaaa
       61 atggggcatt cagttctgct tgtctgcagg gaaaacagca aaattgcttt tgaagccagt
      121 aaattgggaa ttgatatcac attcgcgtta tttcgaaaca gtcttcacat ccctactgca
      181 tggagattac tcggaatagt tcatggtttt cagcccaatg caatcgtttg tcacagtggg
      241 catgatagca atattgttgg tttagtacgg ttattcactt ggaaacatcc attcagaatt
      301 atcaggcaaa agacatattt gacacaaaaa acaaaagttt tttcaataaa tcatttttgc
      361 gatgaggtga ttgttcccgg aacaagtatg aagacacatc tggagcagga aggatgtcga
      421 acccgggtta ctgttgtgcc tccaggcttt gatttccaga aattatacgt tgattcccga
      481 aacagtttgc ctccaaatgt tctttcttgg ctggcgtccc gaaggggatg ccccgt
//
LOCUS       DAJVUJ010000374          535 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.380, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000374 DAJVUJ010000000
VERSION     DAJVUJ010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..535
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.380"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..>535
                     /locus_tag="OQQ48_004360"
     CDS             <1..>535
                     /locus_tag="OQQ48_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HCS1954866.1"
                     /translation="VEERQRGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETA
                     RLRYGYSSMTTPDTLFELDMDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPV
                     SLVYHRKHFRKGHNPLLVYGYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELG
                     QQWYEDGKFLKKKNTFND"
BASE COUNT          129 a          115 c          143 g          148 t
ORIGIN      
        1 ggttgaagag cgtcagcgcg ggttaaccag tttgcgccaa attaaccgca agacccggga
       61 agtcattggt attgcctttg atgatccagc ctatgtgacc tggattgcct acaatccaga
      121 acctgaaacc gcgcgattgc gttatggtta ttcttccatg accacaccag acactttgtt
      181 tgaactggat atggataccg gtgagcgtcg tgtattaaaa caaacggaag ttcctggttt
      241 tgatgcggcg aattaccgca gtgaacacct gtggatagtc gcccgtgatg gcgtcgaagt
      301 tccggtttcg ttggtctacc atcgcaaaca ttttcgcaaa ggacacaacc cgttgctggt
      361 gtatggctat ggttcttacg gcgcaagtat tgatgccgat ttcagtttta gccgcttgag
      421 tttgttagat cgtggctttg tctacgccat tgtccatgtt cgcggcggtg gtgagctggg
      481 gcaacaatgg tacgaagacg gaaaatttct gaagaagaaa aatacgttta atgat
//
LOCUS       DAJVUJ010000375          517 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.383, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000375 DAJVUJ010000000
VERSION     DAJVUJ010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.383"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..>517
                     /locus_tag="OQQ48_004361"
     CDS             <1..>517
                     /locus_tag="OQQ48_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HCS1954867.1"
                     /translation="LANAKYTAQDATTAQKGIVQLSSATNSTSETLAATSKAVKAVMD
                     ETNKKAPLNSPALTGTPTTPAARQGTNNTQIASTAFVMAAIAALVDSSPDALNTLNEL
                     AAALGNAPNFATTMTNALAGKQPKDATLTALAGLATAADRFPYFTGNDVASLATLTKV
                     GRDILAKSTVAA"
BASE COUNT          137 a          148 c          142 g           90 t
ORIGIN      
        1 cttgctaacg caaaatatac cgctcaggac gccacgacgg cacaaaaagg gatagtccag
       61 ctcagtagtg ccaccaacag cacgtctgaa acactggccg cgacatcgaa agcggttaag
      121 gcggtaatgg atgaaacgaa caagaaagcg cccttaaaca gtcctgcgct gaccggaacg
      181 ccaacaacgc cagctgcgcg acagggaacg aataataccc aaatcgcaag cacggctttc
      241 gttatggctg cgattgccgc ccttgtagat tcgtcacctg acgcactgaa tacgctgaac
      301 gagttagcgg cggcgctggg aaacgccccg aattttgcga ccaccatgac taacgcgctt
      361 gcgggtaagc aaccgaaaga tgccaccctg acggcgctgg ccgggcttgc tactgcggca
      421 gacaggtttc cgtattttac ggggaatgat gtcgccagcc tggcaaccct gacaaaagtc
      481 gggcgggata ttcttgcgaa atcgaccgtt gctgccg
//
LOCUS       DAJVUJ010000376          515 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.384, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000376 DAJVUJ010000000
VERSION     DAJVUJ010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.384"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            235..515
                     /locus_tag="OQQ48_004362"
                     /pseudo
     CDS             235..515
                     /locus_tag="OQQ48_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          132 a          136 c          126 g          121 t
ORIGIN      
        1 tccccgccag cccggctaag atggaggcac ggcacagccc gcacacgacg ctgaggctac
       61 agagctgaaa acccacaagg ccatgccccg catggccggg taaagcggag ccggcggcga
      121 cttcctttgt gctctgtccg ggaccggcag gaaaaaggcg gggataatga gatggtcacc
      181 cccaccctaa cagcagtacg ctgttaaggt gaatccctta acggagagac catcatacag
      241 aacatcacac ttattggtat cgatctcgga aagcattcct tccacattca ttgtcaagac
      301 aagtctggta aggctcttct gcgtaaaaaa tttacccagt actaaactga tgcagtttct
      361 ggcaacatgt ccgccctcag ttgttgtaat ggaagcctgt gcgggtgcac actttttggc
      421 tcgccgtatc tctgaatttt ggacatgaaa ctaagctgat ttctccacag ttcgttcgcc
      481 cgtttgtcaa aagtaataag aatgattttg ttgat
//
LOCUS       DAJVUJ010000377          514 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.385, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000377 DAJVUJ010000000
VERSION     DAJVUJ010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.385"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..279
                     /locus_tag="OQQ48_004363"
     CDS             <1..279
                     /locus_tag="OQQ48_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HCS1954868.1"
                     /translation="SLNVYCRNGQVEIDNNIGENALRSVAVGRKNYLFFGSDNGGESA
                     AIIYSLLVTCKLNGVEPEDWLREVIVKLNDWPSNRVHELLPWNFSAVK"
BASE COUNT          125 a          115 c          139 g          135 t
ORIGIN      
        1 agcctgaacg tgtactgtcg taatggccag gtggaaatcg acaacaacat cggtgaaaac
       61 gcgctgcgat cggtggcggt tggaagaaaa aattatctct tcttcggttc tgacaacggt
      121 ggagaaagcg cggcgattat ctacagtctg ctggtcacct gcaaactgaa cggagtggag
      181 ccggaggact ggttgcgcga agtgatcgtg aagctcaatg actggccgtc gaaccgagtg
      241 catgaactgc tgccctggaa cttctcagct gtaaaataat ccttccgcta cgtacttctc
      301 tgggcgctta ccactcaacg gcatcgtcaa aattatcaaa gcgcagaaca tagatttatc
      361 atgtgtttta tggatggctt tctccatcag gcatcgttcg tcacgggaaa ttggtgctat
      421 gatcggcatt gatcagtccg gttggtgatt tgttttgatt tggcgattga tcagatcgca
      481 caatccgggc tgagttccct ctaagtgatc tagg
//
LOCUS       DAJVUJ010000378          511 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.386, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000378 DAJVUJ010000000
VERSION     DAJVUJ010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.386"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..>511
                     /locus_tag="OQQ48_004364"
     CDS             <1..>511
                     /locus_tag="OQQ48_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCS1954869.1"
                     /translation="NDRYRKRTILGCVVATMQAWQYNAVGVYLPLTLAGIISGGLTGA
                     LTGSAVVNALCGVTGGMIGSFILQRLGTRRQSMYGFAVVTLALLSLGALATTNPWLSL
                     GLLGSIIFFHSAGPGGLGMTIATLSYLPAIRPTGVGFARAIMRTGAIAGLIFWPMLWG
                     ALKTEAFYWL"
BASE COUNT           89 a          122 c          148 g          152 t
ORIGIN      
        1 aatgatcgtt accgcaaacg caccattctt ggctgtgtcg tggcaaccat gcaggcgtgg
       61 caatataacg ccgtaggtgt ttatcttcct cttacgttgg cgggaataat aagtggcggg
      121 cttactggcg cgttgaccgg ttctgccgtc gtgaatgccc tttgtggggt gacaggcggg
      181 atgatcggct cgtttattct ccaacgactg ggtactcgcc gacagtcgat gtatggattt
      241 gctgttgtga ccttagcatt gctgtcgtta ggcgcactgg caacgactaa tccctggctg
      301 tctttagggt tattgggatc aattattttc ttccattcag cgggtcctgg tgggctgggc
      361 atgaccattg ccacactctc ttatcttccc gctattcgcc ctactggggt cggatttgcc
      421 cgcgctatta tgcgcacagg ggcaattgca ggactcattt tctggccgat gctgtggggt
      481 gcgttgaaaa ctgaagcgtt ttactggttg g
//
LOCUS       DAJVUJ010000379          461 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.387, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000379 DAJVUJ010000000
VERSION     DAJVUJ010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.387"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(<1..>461)
                     /locus_tag="OQQ48_004365"
     CDS             complement(<1..>461)
                     /locus_tag="OQQ48_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSso6 family transposase"
                     /protein_id="HCS1954870.1"
                     /translation="LKSFLLVHGVHYVGRADWGPAHRRWLSKYSFESPWRQLAFDEHR
                     RTIEDRQAQCERLESALKEAVTEWRLYPVVEALQAMRGIQFITAVGLISELGDLTRFE
                     HPRQLMSWFGITPSEYSSGGSRHQGSITKAGNSYARKLLVEAAWSYRHPAR"
BASE COUNT          106 a          129 c          110 g          116 t
ORIGIN      
        1 atgcgtgccg gatgacggta actccacgct gcctcgacca atagctttct ggcatagctg
       61 tttccggctt tggtaatgct gccctgatgt ctgcttccac ccgatgaata ttctgatggc
      121 gtgataccaa accatgacat cagttgtcgt ggatgttcaa aacgggtcag atcacccagt
      181 tcagaaatta atccaaccgc agtaataaac tgaatccctc gcatggcttg cagcgcctct
      241 actaccggat acagcctcca ttcggtaacg gcctccttca gggcggattc cagccgttca
      301 cattgtgcct gccggtcttc tattgtacga cggtgttcgt caaaagctaa ttgtcgccag
      361 ggactttcga atgagtattt gctgagccag cgtcggtgtg cagggcccca atctgctcgc
      421 ccgacataat ggaccccatg aaccagaaga aacgatttca g
//
LOCUS       DAJVUJ010000380          454 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.388, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000380 DAJVUJ010000000
VERSION     DAJVUJ010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.388"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..172
                     /locus_tag="OQQ48_004366"
     CDS             <1..172
                     /locus_tag="OQQ48_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates concomitant with their translocation across the
                     cell membrane; involved in N,N'-diacetylchitobiose
                     transport; protein IIA transfers a phosphoryl group to IIB
                     which then transfers the phosphoryl group to the sugar;
                     IIC forms the translocation channel for the sugar uptake;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HCS1954871.1"
                     /translation="GAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKE
                     ESEEDIANALKF"
     gene            223..>454
                     /gene="chbA"
                     /locus_tag="OQQ48_004367"
     CDS             223..>454
                     /gene="chbA"
                     /locus_tag="OQQ48_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HCS1954872.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAG"
BASE COUNT          130 a           97 c          131 g           96 t
ORIGIN      
        1 gggagccttc tttaacacca acggtagcgt cgctgcattg ctggtcgcac tcttcaacct
       61 tggtatcgca acgttaattt atctgccctt tgttgtggtg gctaacaaag cgcaaaacgc
      121 gattgataaa gaagagagcg aagaagatat cgctaacgcc ctgaaatttt aatcgatgcc
      181 ggtacggtta atcgtgccgg taagaagaga gaggaacgat gtatgatgga tctcgataac
      241 attcccgata cgcaaacaga agctgaagag ctggaagaag tggtgatggg gcttatcatc
      301 aactccggac aagcgcgcag cctggcgtat gcggcactga aacaggcgaa gcagggcgat
      361 tttgccgcag caaaagccat gatggatcag tcacggatgg cattgaatga agcgcatctg
      421 gtacagacga aactgattga aggcgatgcg ggcg
//
LOCUS       DAJVUJ010000381          452 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.389, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000381 DAJVUJ010000000
VERSION     DAJVUJ010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.389"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            109..>452
                     /locus_tag="OQQ48_004368"
     CDS             109..>452
                     /locus_tag="OQQ48_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1954873.1"
                     /translation="MNNNNTLYVGLDVHKESITVAYAINSEPVELMGKIGTSPTDIQN
                     LCKRLRSKSSQVSIVYEAGPCGYGLYRRLVKSGFDCMVCAPSLIPKKPGERVKTDRRD
                     AIRLVRSLRAGDL"
BASE COUNT          113 a          105 c          117 g          117 t
ORIGIN      
        1 gagacagatg ctggccaacg tgggatattt aacgcgggtt aacatcgttg tcagagccgc
       61 atgggtgagc tgttgctaca ccccggtatt tccaccacgg aggccagcat gaacaacaat
      121 aacacattgt acgtcgggtt agatgttcac aaagaatcga ttactgtcgc ttatgctatc
      181 aattcagaac ctgttgaact gatgggtaaa attggcacat cacctactga tattcagaat
      241 ctttgtaaac gtctcaggtc aaagtcatcg caggtcagta tcgtttatga agcggggccc
      301 tgtggttatg gactttatcg tcggctggtg aaatccggtt ttgactgcat ggtctgcgcc
      361 ccctcactta tcccgaaaaa accgggggag cgggttaaaa ccgatcgccg tgatgccatc
      421 agacttgtgc gttcactgcg tgcaggagat ct
//
LOCUS       DAJVUJ010000382          443 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.390, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000382 DAJVUJ010000000
VERSION     DAJVUJ010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.390"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(29..>443)
                     /locus_tag="OQQ48_004369"
     CDS             complement(29..>443)
                     /locus_tag="OQQ48_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1954874.1"
                     /translation="RLAARKARAAPLMQSLYDRIQQQMKTLSRHSDTAKAFAYLLKQW
                     KALNVYCSNGWVEIDNNIAENALRGVAVGRKNWLFAGSDSGGEHAVVLYSLIGTCRLN
                     NVEPEKWLRYVIEHLQDWPANRVRDLLPWNEVYWQ"
BASE COUNT           91 a          130 c          107 g          115 t
ORIGIN      
        1 ttaaaaaaag aacaaatggt agtgtccgct attgccagta cacctcattc cagggcaaca
       61 gatcgcgtac ccggtttgcc ggccagtcct ggagatgctc aataacgtaa cgcagccatt
      121 tttctggctc tacattgttc agacggcatg tgccgatcag cgagtacaac accaccgcat
      181 gttcgccacc gctgtcggaa cccgcgaaga gccagttttt ccggcctacg gccactcccc
      241 gtaaggcgtt ctctgcgatg ttgttgtcga tttccaccca gccattactg cagtacacgt
      301 tcagggcctt ccactgtttc agtaggtatg cgaacgcttt tgccgtatcc gagtgacgcg
      361 acagtgtttt catctgttgc tgtatccggt catacagtga ctgcatcagt ggcgcggctc
      421 ttgcttttct tgccgccaga cgc
//
LOCUS       DAJVUJ010000383          424 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.392, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000383 DAJVUJ010000000
VERSION     DAJVUJ010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.392"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            1..424
                     /locus_tag="OQQ48_004370"
                     /pseudo
     CDS             1..424
                     /locus_tag="OQQ48_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          100 a          107 c          123 g           94 t
ORIGIN      
        1 ctgcctctga acttgctgcc gccatgaagc agattaaaga actccagcgc ctgctcggca
       61 agaaaacgat ggaaaatgaa ctcctcaaag aagccgttga atatggacgg gcaaaaaagt
      121 ggatagcgca cgcgccctta ttgcccgggg atggggagta agcttagtca gccgttgtct
      181 ccgggtgtcg cgtgcgcagt tgcacgtcat tctcagacga accgatgact ggatggatgg
      241 ccgccgcagt cgtcacactg atgatacgga tgtgcttctc cgtatacacc atgttatcgg
      301 agagctgccc acgtatggtt atcgtcgggt atgggcgctg cttcgcagac aggcagaact
      361 tgatggtatg cctgcgatca atgccaaacg tgtttaccgg atcatgcgcc agaatgcgct
      421 gttg
//
LOCUS       DAJVUJ010000384          422 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.393, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000384 DAJVUJ010000000
VERSION     DAJVUJ010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..422
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.393"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..>422
                     /locus_tag="OQQ48_004371"
     CDS             <1..>422
                     /locus_tag="OQQ48_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCS1954875.1"
                     /translation="NWVQHEPSDRKQSDERLKLEIKVAHIRTRETYGTRRLQTELAEN
                     GIIVGRDRLARLRKELRLRCKQKRKFRATTNPNHNLPVAPNLLNQTFAPTAPNQVWVA
                     DLTYVATQEGWLYLAGIKDVYTCEIVGYAMGERMTKELT"
BASE COUNT          121 a          106 c          118 g           77 t
ORIGIN      
        1 aactgggtac agcatgaacc ctcagacaga aaacaaagtg atgagcggct aaaactggag
       61 attaaggtgg cacatatccg cactcgcgaa acatatggaa cccggcggct ccagacggag
      121 ctggcagaga atggcatcat cgttggtcgt gaccgactgg cacgtcttcg taaggagcta
      181 aggctacgct gtaagcagaa acgcaagttc agagcgacta cgaacccgaa ccacaatctg
      241 ccagttgcgc caaatctgct gaaccagacg ttcgctccta cagcaccaaa tcaggtctgg
      301 gtggcggacc tgacgtatgt tgccacacag gagggatggt tgtacctcgc tggcatcaaa
      361 gatgtttata cgtgcgaaat tgtcggctac gccatgggag agcgcatgac aaaagagctg
      421 ac
//
LOCUS       DAJVUJ010000385          414 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.394, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000385 DAJVUJ010000000
VERSION     DAJVUJ010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.394"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(117..389)
                     /locus_tag="OQQ48_004372"
     CDS             complement(117..389)
                     /locus_tag="OQQ48_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HCS1954876.1"
                     /translation="MVHDRIAEELEAKGFYRRAAARWGEVMQLVETDKERHHITMRRL
                     ECSRKAQRAPEPPDNFGDLKKAVDRTYAEMGIDGAGDEIWRNYQDS"
BASE COUNT           79 a          133 c           85 g          117 t
ORIGIN      
        1 cctctgttgt gtctgtttgt ttcagatagt aactatgcct gaccgtgacg aaaacccggt
       61 aatgcgccat accgtttcaa ctggtgcaaa aaagccggat ttctccggct gtttgattag
      121 ctgtcctggt aattgcgcca tatttcatca ccagcaccat ctatacccat ttcggcataa
      181 gtgcgatcga ctgccttttt caggtctccg aaattatccg gcggctccgg tgccctctgt
      241 gccttccttg aacattccag ccgtcgcatc gtgatgtgat gccgttcctt gtctgtctcc
      301 accagctgca tgacttcacc ccatcgcgcc gccgccctcc ggtaaaagcc tttcgcctcc
      361 agttcctccg ctatgcggtc atgtaccatc gtcaccccct cagaacgaaa tatc
//
LOCUS       DAJVUJ010000386          410 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.396, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000386 DAJVUJ010000000
VERSION     DAJVUJ010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..410
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.396"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            46..410
                     /locus_tag="OQQ48_004373"
                     /pseudo
     CDS             46..410
                     /locus_tag="OQQ48_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          112 a           88 c          103 g          107 t
ORIGIN      
        1 tactgcaccc attttgttgg acgatgaaat ggaatagccc ctaatatgtc aaagccaaaa
       61 tacccttttg aaaagcgcct tgaagtcgtg aatcactact tcactactga tgatggttac
      121 aggatcatct cggcacgttt tggtgtcccc gaacccaggt caggacatgg gttgccctct
      181 atgaaaaaca tggagaaaaa ggcttaattc ccaaacctaa aggtgttagt gctgatccag
      241 agttgcgtat taaggtcgtg aaagctgtga ttgagcagca catgtccctt aatcaggctg
      301 ctgctcactt tatgcttgct ggtagtggtt ctgtagccag gtggctgaag gtttatgaag
      361 agcgcggaga agctggttta cgcgcgctca agattggcac caaaagaaac
//
LOCUS       DAJVUJ010000387          404 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.397, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000387 DAJVUJ010000000
VERSION     DAJVUJ010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.397"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..>404
                     /locus_tag="OQQ48_004374"
     CDS             <1..>404
                     /locus_tag="OQQ48_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HCS1954877.1"
                     /translation="LILFQFAAFVGLLASRENLVGVMPFNLGTLINYQALLVGNLIGV
                     PLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFALWLLALGALLLLLCMPLNEKS
                     TIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWA"
BASE COUNT           96 a           81 c           93 g          134 t
ORIGIN      
        1 ctgatacttt tccagtttgc tgcatttgta ggattattgg cgagcagaga aaatctggtc
       61 ggtgtcatgc cttttaacct cgggacttta atcaattatc aggccttact ggtgggtaat
      121 ctgatcggtg tcccgctgtg ctacttcatc attcgggtag tgcgaaatcc attttattta
      181 cgtagctatt attcgcaatt aaaacagcag gttgatgcca aagtcaccaa aaaagagttc
      241 gcgctctggc tactggcatt aggtgcttta ttgttgctgt tatgcatgcc gttaaatgaa
      301 aaaagcacaa tttttagcac caattatacc ttgtcattat tgctgcccct gatgatgtgg
      361 ggagcgatgc gctatggtta taagctgatt tctctgctct gggc
//
LOCUS       DAJVUJ010000388          395 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.398, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000388 DAJVUJ010000000
VERSION     DAJVUJ010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.398"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..102
                     /locus_tag="OQQ48_004375"
     CDS             <1..102
                     /locus_tag="OQQ48_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCS1954878.1"
                     /translation="IYAKSLQAMDFSAITDKSSYNAIEWPVSPEGSS"
     gene            126..>395
                     /locus_tag="OQQ48_004376"
     CDS             126..>395
                     /locus_tag="OQQ48_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCS1954879.1"
                     /translation="MSVVISGALTDGAGIPMSGCQIILKSRVNTSEVVMRTRADVVTG
                     NNGEYSFEAQVGKYCVYLKRDWRDEYCVGDIAVYDDSKPGTLNDFL"
BASE COUNT          101 a           90 c          104 g          100 t
ORIGIN      
        1 atttacgcga aatcgctgca ggcgatggat ttcagcgcca tcactgataa atcctcatac
       61 aacgccattg aatggcccgt ctctccggaa ggttcttcct gatttaattt atcgcgagaa
      121 aaactatgtc tgtagtgata tcaggtgcgc tgactgatgg cgcaggcatc cccatgtccg
      181 gatgccagat tattctgaaa tcccgtgtaa acacctcaga agtggtgatg cgtaccaggg
      241 ctgatgtagt gaccggaaat aacggtgaat attcgtttga ggcacaggtc ggaaaatatt
      301 gcgtgtatct gaaacgggac tggcgcgacg agtactgtgt tggcgacatt gctgtatacg
      361 acgactcaaa gcccggcacg ctgaatgact ttctg
//
LOCUS       DAJVUJ010000389          394 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.399, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000389 DAJVUJ010000000
VERSION     DAJVUJ010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.399"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            126..>394
                     /locus_tag="OQQ48_004377"
     CDS             126..>394
                     /locus_tag="OQQ48_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1954880.1"
                     /translation="MLPSISINNTSATYPESINENNNDEVNGLVQELKNLFNGKEGIS
                     TCIKHLLELIKNAIRVNDDPYRFNINNSSVTYIDIGSNDTDHITIG"
BASE COUNT          150 a           67 c           55 g          122 t
ORIGIN      
        1 ccctggctat tgtgcgctca tacactcaaa ttaaagatag gttctaaata aatgagcgtt
       61 ttttgatagt ctatttcatt aggtaatata tatttgtaac aaatcaatca aaatggaata
      121 aaatcatgct accatctatt tcaatcaaca ataccagcgc aacttaccca gaatccatta
      181 atgaaaataa caatgatgaa gttaatggat tagtacaaga gctcaaaaac ctttttaatg
      241 gtaaggaagg aataagcacc tgtattaaac atctacttga gcttataaaa aacgccatac
      301 gagtaaacga cgatccttat agatttaata ttaataattc ctcagttact tatattgata
      361 ttggctccaa tgatacagac catattacta ttgg
//
LOCUS       DAJVUJ010000390          393 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.400, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000390 DAJVUJ010000000
VERSION     DAJVUJ010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.400"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
BASE COUNT          143 a           53 c           56 g          141 t
ORIGIN      
        1 tttttgttga gctcccatga ttctttcatt tattgttact aattatttta tgttatcatg
       61 atgttagaac acgctctgct ctgtgtctct attttatcta tcatattctg aggattaact
      121 acataacaag gcgcaagata tatatccaaa aacaaataat accgttgcaa taattccttt
      181 cagtaaagga ggtgtgatta atttcagggg atttcagagt gaaaattgta caacatattg
      241 aaatatgttc atttcgttat aatacacaca agaaaatatt aagttagata atatattcta
      301 aataaccata gataaaaatg cacataatgg ggaatatttt atgaaaatac ctgaagcagt
      361 aaatcatatt aatgttcaga ataatattga tct
//
LOCUS       DAJVUJ010000391          383 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.401, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000391 DAJVUJ010000000
VERSION     DAJVUJ010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..383
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.401"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..230
                     /locus_tag="OQQ48_004378"
     CDS             <1..230
                     /locus_tag="OQQ48_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000535347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1954881.1"
                     /translation="LSDSICSLFIVNSNMASISLGNESFSTDDDLEYGYLINTGNHYD
                     VYLPPELFAQAYKLKNKEMNAQLDYLNRYAI"
BASE COUNT          113 a           69 c           72 g          129 t
ORIGIN      
        1 ctctttcaga tagcatttgc tcattattta ttgttaatag taatatggca tcaatctcct
       61 tggggaatga atccttttca acagatgacg accttgagta tggttattta ataaacactg
      121 gcaatcatta tgatgtttac ctccctcctg aactttttgc tcaggcttat aagttaaaaa
      181 ataaggaaat gaatgcgcaa ctcgactatt taaatcgtta tgcaatttaa tggcaaaggc
      241 atatgctaaa aaccattgtt attagtctca cactttttta ttggtaaata ttgtctctgt
      301 attggtaacg ccgcagatat tctgtttagc cacaggtgca attatcagcg gcgtacgcga
      361 ggcaggggct aatcaggcat agt
//
LOCUS       DAJVUJ010000392          376 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.402, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000392 DAJVUJ010000000
VERSION     DAJVUJ010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.402"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(10..86)
                     /locus_tag="OQQ48_004379"
     tRNA            complement(10..86)
                     /locus_tag="OQQ48_004379"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(50..52),aa:Arg,seq:tct)
BASE COUNT          102 a           92 c           88 g           94 t
ORIGIN      
        1 aagaagaaat ggcgcgccct gcaggattcg aacctgcggc ccacgactta gaaggtcgtt
       61 gctctatcca actgagctaa gggcgcgttg ataccgcaat gcggtgtaat cgcgtgaatt
      121 atacggtcaa cccttgctga gtcaatggct tttgatctgg ttgctgaaca agtgaacgac
      181 cgcgtctgat tttctgattt atttcgctat agcggcaaac aaacgcacac cgctgcgcgt
      241 ctgaatcaag aaaacccgta ttttcatgta tcaaagtaca atttaccgcc ctaacggaaa
      301 attgtccgct cctatgagac tggtaactat gaaaccaacg tctgaggtgt actggcaata
      361 gcggacacta ccattt
//
LOCUS       DAJVUJ010000393          374 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.403, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000393 DAJVUJ010000000
VERSION     DAJVUJ010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.403"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..313
                     /locus_tag="OQQ48_004380"
     CDS             <1..313
                     /locus_tag="OQQ48_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HCS1954882.1"
                     /translation="QYTRNSFYIPLMTRLRPMGITVYVETANRHGLRWLHDVANQRKH
                     ETIQARPCDRWLEEQQSMLALPPEKKEYDVHPGENLVNFDKHPLHHPLSIYDSFCRGV
                     A"
     gene            310..>374
                     /locus_tag="OQQ48_004381"
     CDS             310..>374
                     /locus_tag="OQQ48_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCS1954883.1"
                     /translation="MMMELQHQRLMALAGQLQLESL"
BASE COUNT           96 a          111 c           92 g           75 t
ORIGIN      
        1 gcagtacacc cgtaacagtt tttacatccc actaatgact cgcctgcgcc cgatggggat
       61 cactgtctat gttgaaacag ccaaccgcca cggtctgcgc tggctgcacg atgtcgctaa
      121 ccaacgaaag catgaaacaa tccaggcccg tccctgcgat cgctggctcg aagagcagca
      181 gtccatgctg gcactgcctc cggagaaaaa agagtatgac gtgcatcctg gtgaaaatct
      241 ggtgaacttc gataaacacc ccctgcatca tccactctcc atctacgact cattctgcag
      301 aggagtggcg tgatgatgga actgcaacat caacgactga tggcgctcgc cgggcagttg
      361 caactggaaa gcct
//
LOCUS       DAJVUJ010000394          365 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.404, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000394 DAJVUJ010000000
VERSION     DAJVUJ010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.404"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(<1..355)
                     /locus_tag="OQQ48_004382"
     CDS             complement(<1..355)
                     /locus_tag="OQQ48_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCS1954884.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKQARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIK"
BASE COUNT           89 a           82 c           90 g          104 t
ORIGIN      
        1 gcttaatttt gctttcacat ttttgtaagt cttctgccgg attgtgcgac gtctgcaata
       61 tcacgtcgaa accttcctct tcagctttgg cggtgatggc atgcaaaact tcagagaaaa
      121 acggattacc cgccgtagtt ttggtcgaac gggtagaaat caccataatg gcatcaaaac
      181 ccgaagaggt cagcgcgcgg gcctgcttgt ttggctgata ctgtaattct tcaatggccc
      241 gtaacacttt ttcgcgcgct tccggggaga tattagtttg cttattcagc acgcgtgata
      301 cggtggattt tgatacgcct gcaaccctgg caatatcata aatagtaggg gacataggtc
      361 aggaa
//
LOCUS       DAJVUJ010000395          360 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.405, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000395 DAJVUJ010000000
VERSION     DAJVUJ010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.405"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            215..>360
                     /locus_tag="OQQ48_004383"
     CDS             215..>360
                     /locus_tag="OQQ48_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HCS1954885.1"
                     /translation="MQIKVIYSLIDNMVNFKDKNMPAVIDKALDFIGAMDVSAPTPSS
                     MNEST"
BASE COUNT          108 a           76 c           64 g          112 t
ORIGIN      
        1 aactatataa ataaacataa ccatgccgtt gtgttcgccc ttcgattagc acctctggtt
       61 ttctttctta gctgaacaat ctggttatag cgtgcggact gaccgttaat tttctccatc
      121 ttactggata acacaccatg agctgctacc tttaaaacac ctgaatgtca acaggttaac
      181 tcgtgccata tcgttataat ccctttgata ttagatgcaa attaaggtca tatatagcct
      241 tatcgataac atggttaatt ttaaggataa gaatatgcct gctgtaatag ataaagccct
      301 ggatttcatt ggtgccatgg atgtatcagc gccaacacca agttcgatga atgaaagcac
//
LOCUS       DAJVUJ010000396          356 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.406, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000396 DAJVUJ010000000
VERSION     DAJVUJ010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.406"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..346
                     /locus_tag="OQQ48_004384"
     CDS             <1..346
                     /locus_tag="OQQ48_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1954886.1"
                     /translation="VMCLTKPAFSYYIAAYLKLFIENYYEADALVDTILNMLTPPVRN
                     GIPSTTWIAKNLDCFSRNKRVAISFTFQYLCNEYNDPNTEKALNIYWGQYLNESLLIK
                     NVQNSKNKCKSQ"
BASE COUNT          127 a           56 c           54 g          119 t
ORIGIN      
        1 tgtcatgtgt ctcactaaac cagcattctc atattatatt gcagcctatt taaaactttt
       61 tattgaaaat tattacgaag ctgatgcttt agtggatact attttaaata tgctcactcc
      121 tcctgttagg aatggtatcc ccagcactac atggatagca aaaaatcttg attgctttag
      181 tcgtaataaa agggtggcta tttcttttac attccaatat ttatgtaatg aatataatga
      241 tccaaatact gaaaaggcgt taaatatata ttggggacag tatctgaatg aatcattgct
      301 tattaaaaat gtacagaact ctaaaaataa atgtaaatca cagtaagaca gaacca
//
LOCUS       DAJVUJ010000397          338 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.408, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000397 DAJVUJ010000000
VERSION     DAJVUJ010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.408"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
BASE COUNT          122 a           39 c           56 g          121 t
ORIGIN      
        1 tatggatttt taacaagcat atattgttga aatgctttgt tagaatcttt atccattccc
       61 tggggctgta tagtatgatt tttcaggtat ttctgaatac agccaaatat tattgcaaaa
      121 tattctctaa attattagtt aaatatccat aagcattatt gcctgggaac tggacatata
      181 attactcttc tgtgctaaag gaacgttctg aaatgtttaa gtatgcagag atgttgatgg
      241 cggaagaaga taataaagta tgtgaactta tttttaaaca acaagataat ttgattttaa
      301 aaataaatga tagatttccc agaataatta taaagaaa
//
LOCUS       DAJVUJ010000398          332 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.410, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000398 DAJVUJ010000000
VERSION     DAJVUJ010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.410"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(<1..>332)
                     /locus_tag="OQQ48_004385"
     CDS             complement(<1..>332)
                     /locus_tag="OQQ48_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1A family transposase"
                     /protein_id="HCS1954887.1"
                     /translation="VIVCAEMDEQWGYVGAKSRQRWLFYAYDSLRKTVVAHVFGERTM
                     ATLGRLMSLLSPFDVVIWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLAR
                     LGRKSLSFS"
BASE COUNT           73 a          109 c           81 g           69 t
ORIGIN      
        1 gagaacgaca gcgacttccg tcccagccgt gccaggtgct gcctcagatt caggttatgc
       61 cgctcaattc gctgcgtata tcgcttgctg attacgtgca gctttccctt caggcgggat
      121 tcatacagcg gccagccatc cgtcatccat atcaccacgt caaagggtga cagcaggctc
      181 ataagacgcc ccagcgtcgc catagtgcgt tcaccgaata cgtgcgcaac aaccgtcttc
      241 cggagactgt catacgcgta aaacagccag cgctggcgcg atttagcccc gacatagccc
      301 cactgttcgt ccatttccgc gcagacgatg ac
//
LOCUS       DAJVUJ010000399          326 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.411, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000399 DAJVUJ010000000
VERSION     DAJVUJ010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.411"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..>326
                     /locus_tag="OQQ48_004386"
     CDS             <1..>326
                     /locus_tag="OQQ48_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCS1954888.1"
                     /translation="EYPQSAAIDLRCVNMVADLWHAPAPKNGQAVGTNTIGSSEACML
                     GGMAMKWRWRKRMEAAGKPTDKPNLVCGPVQICWHKFARYWDVELREIPMRPGQLFMD
                     PKRMIEA"
BASE COUNT           74 a           85 c           97 g           70 t
ORIGIN      
        1 gaatatccgc aatccgcagc catcgacctg cgttgcgtaa atatggttgc cgatctgtgg
       61 catgcgcctg cgccgaaaaa tggtcaggcc gttggcacca acaccattgg ttcttccgag
      121 gcctgtatgc tcggcgggat ggcgatgaaa tggcgttggc gcaagcgtat ggaagctgca
      181 ggcaaaccaa cggataaacc aaacctggtg tgcggtccgg tacaaatctg ctggcataaa
      241 ttcgcccgct actgggatgt ggagctgcgt gagatcccta tgcgccccgg tcagttgttt
      301 atggacccga aacgcatgat tgaagc
//
LOCUS       DAJVUJ010000400          321 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.412, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000400 DAJVUJ010000000
VERSION     DAJVUJ010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.412"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..>321
                     /locus_tag="OQQ48_004387"
     CDS             <1..>321
                     /locus_tag="OQQ48_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1954889.1"
                     /translation="AITALNFYQGDVPSVMIRIQPENMSPFIIDISTGEHDNYIIQTL
                     DVGTFAPFGEQCTCSAVNKKELECIKETISKYCAKFTQKEAILTAPTHFNKTCITSDC
                     WQILF"
BASE COUNT          106 a           66 c           53 g           96 t
ORIGIN      
        1 gcaattactg cattgaattt ctatcaaggc gatgttcctt ctgtcatgat ccgaatccaa
       61 ccggaaaata tgagtccatt catcatagat atttctacag gagaacatga taattatatc
      121 atacaaacat tagatgtagg cacttttgca ccttttggtg aacaatgtac ttgctcagcc
      181 gtcaataaaa aagagctgga atgtattaag gagacgattt ctaagtattg cgcaaagttc
      241 acccaaaaag aagccatctt aacagcccct acgcacttta ataaaacctg cattacttca
      301 gattgttggc aaattctctt t
//
LOCUS       DAJVUJ010000401          317 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.414, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000401 DAJVUJ010000000
VERSION     DAJVUJ010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.414"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            139..>317
                     /locus_tag="OQQ48_004388"
     CDS             139..>317
                     /locus_tag="OQQ48_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HCS1954890.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTH"
BASE COUNT           96 a           67 c           72 g           82 t
ORIGIN      
        1 tcccgtggca acgctatcct tttgtcacca tgatttccgt cgagcggaaa ggttaagcat
       61 tgtacactcg aggaggccag acaaactggg actggctaca cagctggaaa atcatcagat
      121 aaatgaaggg aaacaaaaat gaaaaaaacg ctcgcaacgt tatttttatt aacgtgtctg
      181 ggatcatcat ccgcctatgc ggataacgct ttgattttac aaactgattt tagcctgaaa
      241 gatggtgccg tctcggcgat gaaaggggtc gcattcggcg ttgaccataa tcttaaaatc
      301 tttgatttaa cacacga
//
LOCUS       DAJVUJ010000402          316 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.415, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000402 DAJVUJ010000000
VERSION     DAJVUJ010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..316
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.415"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            <1..>316
                     /locus_tag="OQQ48_004389"
     CDS             <1..>316
                     /locus_tag="OQQ48_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HCS1954891.1"
                     /translation="YPALVGNDIGCGMALWQTDILARKYNADKFEKRLSDLDDVAEES
                     WLEENLPSAFAQHPWCSSLGSIGGGNHFAELQQVDQIINAELFALAGLDAQHLQLLVH
                     SGS"
BASE COUNT           80 a           75 c           82 g           79 t
ORIGIN      
        1 tttatccggc actggtcggc aatgatatcg gctgcggtat ggcgctatgg caaacagata
       61 ttctcgctcg caaatacaac gccgataagt ttgaaaagcg attatcagat ctggatgacg
      121 ttgctgaaga aagctggctg gaggaaaacc tgccgtcagc atttgcacag catccgtggt
      181 gcagctcgct tggctctatc ggtggcggca atcatttcgc tgaactgcaa caagttgatc
      241 aaatcatcaa cgctgaactg tttgcactag caggtctgga tgcacaacat ctgcaactgt
      301 tggttcatag cggctc
//
LOCUS       DAJVUJ010000403          311 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.denovo.417, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000403 DAJVUJ010000000
VERSION     DAJVUJ010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..311
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.denovo.417"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
BASE COUNT          118 a           47 c           66 g           80 t
ORIGIN      
        1 aggtatgttt ctggtggaaa ggacagtggg attaaaaagt aagaattgat aaaaaaacgc
       61 cagcaacaca tagtgccggc ggagggaata ccccatggag aaaatgtgat gcctagagca
      121 ccatgaatat ataattaaaa atagttagag ttgtcaatac tttcacaaaa ataattttcg
      181 tagtttcgta gtatagaaag atatttttat gcatgaccta cctgaatttg ctccgggtag
      241 aggttataaa taaaaattga atcacgacaa acacaatatt catagtatgg cgatgcctac
      301 gccagcaaga a
//
LOCUS       DAJVUJ010000404         4920 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.guided.220, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000404 DAJVUJ010000000
VERSION     DAJVUJ010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4920
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.guided.220"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            556..1371
                     /gene="sul2"
                     /locus_tag="OQQ48_004390"
     CDS             556..1371
                     /gene="sul2"
                     /locus_tag="OQQ48_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCS1954892.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            1432..1959
                     /locus_tag="OQQ48_004391"
                     /pseudo
     CDS             1432..1959
                     /locus_tag="OQQ48_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
     gene            1980..2453
                     /locus_tag="OQQ48_004392"
     CDS             1980..2453
                     /locus_tag="OQQ48_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA14"
                     /protein_id="HCS1954893.1"
                     /translation="MKVSLIAAKAKNGVIGCGPDIPWSAKGEQLLFKALTYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSGWTSNDDNVVVFQSIEEAMDRLAEFTGHVIVSGGGEI
                     YRETLPMASTLHLSTIDIEPEGDVFFPSIPNTFEVVFEQHFTSNINYCYQIWKKG"
     gene            2528..2803
                     /locus_tag="OQQ48_004393"
                     /pseudo
     CDS             2528..2803
                     /locus_tag="OQQ48_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase APH(3')"
     gene            2803..3639
                     /locus_tag="OQQ48_004394"
     CDS             2803..3639
                     /locus_tag="OQQ48_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(6)-I family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HCS1954894.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMSLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(3746..4150)
                     /locus_tag="OQQ48_004395"
                     /pseudo
     CDS             complement(3746..4150)
                     /locus_tag="OQQ48_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(4194..4583)
                     /locus_tag="OQQ48_004396"
     CDS             complement(4194..4583)
                     /locus_tag="OQQ48_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1954895.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
BASE COUNT         1163 a         1312 c         1268 g         1177 t
ORIGIN      
        1 aacccggaaa gacacgacaa aacgccactg gcagcagcca ttagtaacag gagtgcacga
       61 gaagatagat taagaggtct tgaagtagag gcctgactgc ggctacactg aaagaacagt
      121 tttggtaatg gcggtctcgt gaaccctgtt accgggttaa gcagttcccc aactgactta
      181 accttcgaac aaaccgcctc cccaggcggt tttttcgttt acagagcaag agattacgat
      241 tcattgaaaa ggatctcaag aagatccttt gtagattact atcaattaat cttccgaaca
      301 tgacaccagt cactgttgat tacggttaaa ccgctccgta acaggtttcc ctcgtattgg
      361 tgacttgcag caccaaagaa gcaaaaaaga taaatatatc tacttattga gagatgatat
      421 atttatttcc aaaatgctac agatacatca tcatgaaacg gatcaccgca aacaggttac
      481 tcgcctgggg attccctttc gacccgagca tccgtatgag actcatgctc gattattatt
      541 attatagaag cccccatgaa taaatcgctc atcattttcg gcatcgtcaa cataacctcg
      601 gacagtttct ccgatggagg ccggtatctg gcgccagacg cagccattgc gcaggcgcgt
      661 aagctgatgg ccgagggggc agatgtgatc gacctcggtc cggcatccag caatcccgac
      721 gccgcgcctg tttcgtccga cacagaaatc gcgcgtatcg cgccggtgct ggacgcgctc
      781 aaggcagatg gcattcccgt ctcgctcgac agttatcaac ccgcgacgca agcctatgcc
      841 ttgtcgcgtg gtgtggccta tctcaatgat attcgcggtt ttccagacgc tgcgttctat
      901 ccgcaattgg cgaaatcatc tgccaaactc gtcgttatgc attcggtgca agacgggcag
      961 gcagatcggc gcgaggcacc cgctggcgac atcatggatc acattgcggc gttctttgac
     1021 gcgcgcatcg cggcgctgac gggtgccggt atcaaacgca accgccttgt ccttgatccc
     1081 ggcatggggt tttttctggg ggctgctccc gaaacctcgc tctcggtgct ggcgcggttc
     1141 gatgaattgc ggctgcgctt cgatttgccg gtgcttctgt ctgtttcgcg caaatccttt
     1201 ctgcgcgcgc tcacaggccg tggtccgggg gatgtcgggg ccgcgacact cgctgcagag
     1261 cttgccgccg ccgcaggtgg agctgacttc atccgcacac acgagccgcg ccccttgcgc
     1321 gacgggctgg cggtattggc ggcgctgaaa gaaaccgcaa gaattcgtta actgcacatt
     1381 cgggatattt ctctatattc gcgcttcatc agaaaactga aggaacctcc attgaatcga
     1441 actaatattt tttttggtga atcgcattct gactggttgc ctgtcagagg cggagaatct
     1501 ggtgattttg tttttcgacg tggtgacggg catgccttcg cgaaaatcgc acctgcttcc
     1561 cgccgcggtg agctcgctgg agagcgtgac cgcctcattt ggctcaaagg tcgaggtgtg
     1621 gcttgccccg aggtcatcaa ctggcaggag gaacaggagg gtgcatgctt ggtgataacg
     1681 gcaattccgg gagtaccggc ggctgatctg tctggagcgg atttgctcaa agcgtggccg
     1741 tcaatggggc agcaacttgg cgctgttcac agcctatcgg ttgatcaatg tccgtttgag
     1801 cgcaggctgt cgcgaatgtt cggacgcgcc gttgatgtgg tgtcccgcaa tgccgtcaat
     1861 cccgacttct taccggacga ggacaagagt acgccgcagc tcgatctttt ggctcgtgtc
     1921 gaacgagagc taccggtgcg gctcgaccaa gagcgcaccg ttaacccagg atgagaacct
     1981 tgaaagtatc attgatagct gcgaaagcga aaaacggcgt gattggttgc ggtccagaca
     2041 taccctggtc cgcgaaaggg gagcagctac tttttaaagc attgacctac aatcagtggc
     2101 ttctggtggg tcgcaagacg tttgaatcta tgggcgcact ccccaatagg aaatacgcgg
     2161 tcgttacccg ctcaggttgg acatcaaatg atgacaatgt agttgtattt cagtcaatcg
     2221 aagaggccat ggacaggcta gctgaattca ccggtcacgt tatagtgtct ggtggcggag
     2281 aaatttaccg agaaacatta cccatggcct ctacgctcca cttatcgacg atcgacatcg
     2341 agccagaggg ggatgttttc ttcccgagta ttccaaatac cttcgaagtt gtttttgagc
     2401 aacactttac ttcaaacatt aactattgct atcaaatttg gaaaaagggt taacaaagct
     2461 atgcaatcga cggcaaaaag cttcgttcgc ttcgcgcact acgccttttt ccgcgattga
     2521 tagcgacgat atggttgttt gccatggtga tccctgcatg ccgaacttca tggtggaccc
     2581 taaaactctt caatgcacgg gtctgatcga ccttgggcgg ctcggaacag cagatcgcta
     2641 tgccgatttg gcactcatga ttgctaacgc cgaagagaac tgggcagcgc cagatgaagc
     2701 agagcgcgcc ttcgctgtcc tattcaatgt attggggatc gaagcccccg accgcgaacg
     2761 ccttgccttc tatctgcgat tggaccctct gacttggggt tgatgttcat gccgcctgtt
     2821 tttcctgctc attggcacgt ttcgcaacct gttctcattg cggacacctt ttccagcctc
     2881 gtttggaaag tttcattgcc agacgggact cctgcaatcg tcaagggatt gaaacctata
     2941 gaagacattg ctgatgaact gcgcggggcc gactatctgg tatggcgcaa tgggagggga
     3001 gcagtccggt tgctcggtcg tgagaacaat ctgatgtcgc tcgaatatgc cggggagcga
     3061 atgctctctc acatcgttgc cgagcacggc gactaccagg cgaccgaaat tgcagcggaa
     3121 ctaatggcga agctgtatgc cgcatctgag gaacccctgc cttctgccct tctcccgatc
     3181 cgggatcgct ttgcagcttt gtttcagcgg gcgcgcgatg atcaaaacgc aggttgtcaa
     3241 actgactacg tccacgcggc gattatagcc gatcaaatga tgagcaatgc ctcggaactg
     3301 cgtgggctac atggcgatct gcatcatgaa aacatcatgt tctccagtcg cggctggctg
     3361 gtgatagatc ccgtcggtct ggtcggtgaa gtgggctttg gcgccgccaa tatgttctac
     3421 gatccggctg acagagacga cctttgtctc gatcctagac gcattgcaca gatggcggac
     3481 gcattctctc gtgcgctgga cgtcgatccg cgtcgcctgc tcgaccaggc gtacgcttat
     3541 gggtgccttt ccgcagcttg gaacgcggat ggagaagagg agcaacgcga tctagctatc
     3601 gcggccgcga tcaagcaggt gcgacagacg tcatactaga tatcaagcga cttctcctat
     3661 cccctgggaa cacatcaatc tcaccggaga atatcgctgg ccaaagcctt agcgtaggat
     3721 tccgcccctt cccgcaaacg accccaaaca ggaaacgcag ctgaaacggg aagctcaaca
     3781 cccactgacg catgggttgt tcaggcagta cttcatcaac cagcaaggcg gcactttcgg
     3841 ccatccgccg cgccccacag ctcgggcaga aaccgcgacg cttacagctg aaagcgacca
     3901 ggtgctcggc gtggcaagac tcgcagcgaa cccgtagaaa gccatgctcc agccgcccgc
     3961 attggagaaa ttcttcaaat tcccgttgca catagcccgg caattccttt ccctgctctg
     4021 ccataagcgc agcgaatgcc gggtaatact cgtcaacgat ctgatagaga agggtttgct
     4081 cgggtcggtg gctctggtaa cgaccagtat cccgatcccg gctggccgtc ctggccgcca
     4141 catgaggcat gttccgcgtc cttgcaatac tgtgtttaca tacagtctat cgcttagcgg
     4201 aaagttcttt taccctcagc cgaaatgcct gccgttgcta gacattgcca gccagtgccc
     4261 gtcactccca ctcttacatt gttcgcagta tctaggtaca tgttattgcg tgcaccttca
     4321 tgatgtataa tgttattaat gacagcagaa agatcgacag aattaatatt cttcgtacct
     4381 gggacagtta tcgggtgtcg gccagtgatg agaccaaagg atgcagtata agatgaacca
     4441 ctactattat catggccacc attaccacca ccgttaaaac tacctcccga acttccagag
     4501 ccgctatgac catcattgta ggggtcattt ggtcccatgt gaccaaaacc accgacagta
     4561 ccgctattta actctgtact cataaaatta tcctctttga cttttaaaaa cagcaaaaac
     4621 tgtatttata acaatgtgtt tatatacagt attcgaaata ctacgcccgt caaaaccaca
     4681 ccgtcaacag gttttttgtg tatttttcac gcctcacggg cagcctcaga ccttttgtgt
     4741 taaaacagta atatgttctg ctgttacaat acggtacaca caggagacac tgtcactttt
     4801 atggcatggc acacagttca agagacactg aaattaacag gaaagtcgcg ctcgcaatta
     4861 tatcgtgaca tggctaaagg ccttgtcagc tatcgcacag gaaaaaatga tcgccgggag
//
LOCUS       DAJVUJ010000405         4920 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.guided.221, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000405 DAJVUJ010000000
VERSION     DAJVUJ010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4920
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.guided.221"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            556..1371
                     /gene="sul2"
                     /locus_tag="OQQ48_004397"
     CDS             556..1371
                     /gene="sul2"
                     /locus_tag="OQQ48_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCS1954896.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            1432..1959
                     /locus_tag="OQQ48_004398"
                     /pseudo
     CDS             1432..1959
                     /locus_tag="OQQ48_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
     gene            1980..2453
                     /locus_tag="OQQ48_004399"
     CDS             1980..2453
                     /locus_tag="OQQ48_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA14"
                     /protein_id="HCS1954897.1"
                     /translation="MKVSLIAAKAKNGVIGCGPDIPWSAKGEQLLFKALTYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSGWTSNDDNVVVFQSIEEAMDRLAEFTGHVIVSGGGEI
                     YRETLPMASTLHLSTIDIEPEGDVFFPSIPNTFEVVFEQHFTSNINYCYQIWKKG"
     gene            2528..2803
                     /locus_tag="OQQ48_004400"
                     /pseudo
     CDS             2528..2803
                     /locus_tag="OQQ48_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase APH(3')"
     gene            2803..3639
                     /locus_tag="OQQ48_004401"
     CDS             2803..3639
                     /locus_tag="OQQ48_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HCS1954898.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(3746..4150)
                     /locus_tag="OQQ48_004402"
                     /pseudo
     CDS             complement(3746..4150)
                     /locus_tag="OQQ48_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(4194..4583)
                     /locus_tag="OQQ48_004403"
     CDS             complement(4194..4583)
                     /locus_tag="OQQ48_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCS1954899.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
BASE COUNT         1163 a         1311 c         1268 g         1178 t
ORIGIN      
        1 aacccggaaa gacacgacaa aacgccactg gcagcagcca ttagtaacag gagtgcacga
       61 gaagatagat taagaggtct tgaagtagag gcctgactgc ggctacactg aaagaacagt
      121 tttggtaatg gcggtctcgt gaaccctgtt accgggttaa gcagttcccc aactgactta
      181 accttcgaac aaaccgcctc cccaggcggt tttttcgttt acagagcaag agattacgat
      241 tcattgaaaa ggatctcaag aagatccttt gtagattact atcaattaat cttccgaaca
      301 tgacaccagt cactgttgat tacggttaaa ccgctccgta acaggtttcc ctcgtattgg
      361 tgacttgcag caccaaagaa gcaaaaaaga taaatatatc tacttattga gagatgatat
      421 atttatttcc aaaatgctac agatacatca tcatgaaacg gatcaccgca aacaggttac
      481 tcgcctgggg attccctttc gacccgagca tccgtatgag actcatgctc gattattatt
      541 attatagaag cccccatgaa taaatcgctc atcattttcg gcatcgtcaa cataacctcg
      601 gacagtttct ccgatggagg ccggtatctg gcgccagacg cagccattgc gcaggcgcgt
      661 aagctgatgg ccgagggggc agatgtgatc gacctcggtc cggcatccag caatcccgac
      721 gccgcgcctg tttcgtccga cacagaaatc gcgcgtatcg cgccggtgct ggacgcgctc
      781 aaggcagatg gcattcccgt ctcgctcgac agttatcaac ccgcgacgca agcctatgcc
      841 ttgtcgcgtg gtgtggccta tctcaatgat attcgcggtt ttccagacgc tgcgttctat
      901 ccgcaattgg cgaaatcatc tgccaaactc gtcgttatgc attcggtgca agacgggcag
      961 gcagatcggc gcgaggcacc cgctggcgac atcatggatc acattgcggc gttctttgac
     1021 gcgcgcatcg cggcgctgac gggtgccggt atcaaacgca accgccttgt ccttgatccc
     1081 ggcatggggt tttttctggg ggctgctccc gaaacctcgc tctcggtgct ggcgcggttc
     1141 gatgaattgc ggctgcgctt cgatttgccg gtgcttctgt ctgtttcgcg caaatccttt
     1201 ctgcgcgcgc tcacaggccg tggtccgggg gatgtcgggg ccgcgacact cgctgcagag
     1261 cttgccgccg ccgcaggtgg agctgacttc atccgcacac acgagccgcg ccccttgcgc
     1321 gacgggctgg cggtattggc ggcgctgaaa gaaaccgcaa gaattcgtta actgcacatt
     1381 cgggatattt ctctatattc gcgcttcatc agaaaactga aggaacctcc attgaatcga
     1441 actaatattt tttttggtga atcgcattct gactggttgc ctgtcagagg cggagaatct
     1501 ggtgattttg tttttcgacg tggtgacggg catgccttcg cgaaaatcgc acctgcttcc
     1561 cgccgcggtg agctcgctgg agagcgtgac cgcctcattt ggctcaaagg tcgaggtgtg
     1621 gcttgccccg aggtcatcaa ctggcaggag gaacaggagg gtgcatgctt ggtgataacg
     1681 gcaattccgg gagtaccggc ggctgatctg tctggagcgg atttgctcaa agcgtggccg
     1741 tcaatggggc agcaacttgg cgctgttcac agcctatcgg ttgatcaatg tccgtttgag
     1801 cgcaggctgt cgcgaatgtt cggacgcgcc gttgatgtgg tgtcccgcaa tgccgtcaat
     1861 cccgacttct taccggacga ggacaagagt acgccgcagc tcgatctttt ggctcgtgtc
     1921 gaacgagagc taccggtgcg gctcgaccaa gagcgcaccg ttaacccagg atgagaacct
     1981 tgaaagtatc attgatagct gcgaaagcga aaaacggcgt gattggttgc ggtccagaca
     2041 taccctggtc cgcgaaaggg gagcagctac tttttaaagc attgacctac aatcagtggc
     2101 ttctggtggg tcgcaagacg tttgaatcta tgggcgcact ccccaatagg aaatacgcgg
     2161 tcgttacccg ctcaggttgg acatcaaatg atgacaatgt agttgtattt cagtcaatcg
     2221 aagaggccat ggacaggcta gctgaattca ccggtcacgt tatagtgtct ggtggcggag
     2281 aaatttaccg agaaacatta cccatggcct ctacgctcca cttatcgacg atcgacatcg
     2341 agccagaggg ggatgttttc ttcccgagta ttccaaatac cttcgaagtt gtttttgagc
     2401 aacactttac ttcaaacatt aactattgct atcaaatttg gaaaaagggt taacaaagct
     2461 atgcaatcga cggcaaaaag cttcgttcgc ttcgcgcact acgccttttt ccgcgattga
     2521 tagcgacgat atggttgttt gccatggtga tccctgcatg ccgaacttca tggtggaccc
     2581 taaaactctt caatgcacgg gtctgatcga ccttgggcgg ctcggaacag cagatcgcta
     2641 tgccgatttg gcactcatga ttgctaacgc cgaagagaac tgggcagcgc cagatgaagc
     2701 agagcgcgcc ttcgctgtcc tattcaatgt attggggatc gaagcccccg accgcgaacg
     2761 ccttgccttc tatctgcgat tggaccctct gacttggggt tgatgttcat gccgcctgtt
     2821 tttcctgctc attggcacgt ttcgcaacct gttctcattg cggacacctt ttccagcctc
     2881 gtttggaaag tttcattgcc agacgggact cctgcaatcg tcaagggatt gaaacctata
     2941 gaagacattg ctgatgaact gcgcggggcc gactatctgg tatggcgcaa tgggagggga
     3001 gcagtccggt tgctcggtcg tgagaacaat ctgatgttgc tcgaatatgc cggggagcga
     3061 atgctctctc acatcgttgc cgagcacggc gactaccagg cgaccgaaat tgcagcggaa
     3121 ctaatggcga agctgtatgc cgcatctgag gaacccctgc cttctgccct tctcccgatc
     3181 cgggatcgct ttgcagcttt gtttcagcgg gcgcgcgatg atcaaaacgc aggttgtcaa
     3241 actgactacg tccacgcggc gattatagcc gatcaaatga tgagcaatgc ctcggaactg
     3301 cgtgggctac atggcgatct gcatcatgaa aacatcatgt tctccagtcg cggctggctg
     3361 gtgatagatc ccgtcggtct ggtcggtgaa gtgggctttg gcgccgccaa tatgttctac
     3421 gatccggctg acagagacga cctttgtctc gatcctagac gcattgcaca gatggcggac
     3481 gcattctctc gtgcgctgga cgtcgatccg cgtcgcctgc tcgaccaggc gtacgcttat
     3541 gggtgccttt ccgcagcttg gaacgcggat ggagaagagg agcaacgcga tctagctatc
     3601 gcggccgcga tcaagcaggt gcgacagacg tcatactaga tatcaagcga cttctcctat
     3661 cccctgggaa cacatcaatc tcaccggaga atatcgctgg ccaaagcctt agcgtaggat
     3721 tccgcccctt cccgcaaacg accccaaaca ggaaacgcag ctgaaacggg aagctcaaca
     3781 cccactgacg catgggttgt tcaggcagta cttcatcaac cagcaaggcg gcactttcgg
     3841 ccatccgccg cgccccacag ctcgggcaga aaccgcgacg cttacagctg aaagcgacca
     3901 ggtgctcggc gtggcaagac tcgcagcgaa cccgtagaaa gccatgctcc agccgcccgc
     3961 attggagaaa ttcttcaaat tcccgttgca catagcccgg caattccttt ccctgctctg
     4021 ccataagcgc agcgaatgcc gggtaatact cgtcaacgat ctgatagaga agggtttgct
     4081 cgggtcggtg gctctggtaa cgaccagtat cccgatcccg gctggccgtc ctggccgcca
     4141 catgaggcat gttccgcgtc cttgcaatac tgtgtttaca tacagtctat cgcttagcgg
     4201 aaagttcttt taccctcagc cgaaatgcct gccgttgcta gacattgcca gccagtgccc
     4261 gtcactccca ctcttacatt gttcgcagta tctaggtaca tgttattgcg tgcaccttca
     4321 tgatgtataa tgttattaat gacagcagaa agatcgacag aattaatatt cttcgtacct
     4381 gggacagtta tcgggtgtcg gccagtgatg agaccaaagg atgcagtata agatgaacca
     4441 ctactattat catggccacc attaccacca ccgttaaaac tacctcccga acttccagag
     4501 ccgctatgac catcattgta ggggtcattt ggtcccatgt gaccaaaacc accgacagta
     4561 ccgctattta actctgtact cataaaatta tcctctttga cttttaaaaa cagcaaaaac
     4621 tgtatttata acaatgtgtt tatatacagt attcgaaata ctacgcccgt caaaaccaca
     4681 ccgtcaacag gttttttgtg tatttttcac gcctcacggg cagcctcaga ccttttgtgt
     4741 taaaacagta atatgttctg ctgttacaat acggtacaca caggagacac tgtcactttt
     4801 atggcatggc acacagttca agagacactg aaattaacag gaaagtcgcg ctcgcaatta
     4861 tatcgtgaca tggctaaagg ccttgtcagc tatcgcacag gaaaaaatga tcgccgggag
//
LOCUS       DAJVUJ010000406         3624 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.guided.254, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000406 DAJVUJ010000000
VERSION     DAJVUJ010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3624
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.guided.254"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            complement(<1..579)
                     /gene="tetR(A)"
                     /locus_tag="OQQ48_004404"
     CDS             complement(<1..579)
                     /gene="tetR(A)"
                     /locus_tag="OQQ48_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HCS1954900.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGG
                     TVEQAPLSPLLRAAIDAFDEAGPDAAFEQGLAV"
     gene            685..1884
                     /gene="tet(A)"
                     /locus_tag="OQQ48_004405"
     CDS             685..1884
                     /gene="tet(A)"
                     /locus_tag="OQQ48_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HCS1954901.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSG
                     AGQRADR"
     gene            complement(1916..2800)
                     /locus_tag="OQQ48_004406"
     CDS             complement(1916..2800)
                     /locus_tag="OQQ48_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HCS1954902.1"
                     /translation="MSLRTPDLLFTAIAPAIWGSTYIVTTQYLPNFSPMTVAMLRALP
                     AGLLLVMIVRQIPTGIWWMRIFILGALNISLFWSLLFISVYRLPGGVAATVGAVQPLM
                     VVFISAALLGSPIRLMAVLGAICGTAGVALLVLTPNAALDPVGVAAGLAGAVSMAFGT
                     VLTRKWQPPVPLLTFTAWQLAAGGLLLVPVALVFDPPIPMPTGTNVLGLAWLGLIGAG
                     LTYFLWFRGISRLEPTVVSLLGFLSPGTAVLLGWLFLDQTLSALQIIGVLLVIGSIWL
                     GQRSNRTPRARIACRKSP"
     gene            complement(2938..3345)
                     /locus_tag="OQQ48_004407"
     CDS             complement(2938..3345)
                     /locus_tag="OQQ48_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCS1954903.1"
                     /translation="MRGRPVLFPNFSLGEEYEHAPPATNRQISPYLPSGRFRTGLPVE
                     GLAIERGDLFYACPRASVFYGTALDADLRTRGVSTLVMAGISTTGVVLSSVAWASDAD
                     YDVRLVQDCCYDPDRDAHEALLRSGFGGRVQVV"
     gene            3334..3624
                     /locus_tag="OQQ48_004408"
                     /pseudo
     CDS             3334..3624
                     /locus_tag="OQQ48_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          695 a         1148 c         1110 g          671 t
ORIGIN      
        1 caccgcgagt ccctgctcga acgctgcgtc cggaccggct tcgtcgaagg cgtctatcgc
       61 ggcccgcaac agcggcgaga gcggagcctg ttcaacggtg ccgccgcgct cgccggcatc
      121 gctgtcgccg gcctgctcct caagcacggc cccaacagtg aagtagctga ttgtcatcag
      181 cgcattgacg gcgtccccgg ccgaaaaacc cgcctcgcag aggaagcgaa gctgcgcgtc
      241 ggccgtttcc atctgcggtg cgcccggtcg cgtgccggca tggatgcgcg cgccatcgcg
      301 gtaggcgagc agcgcctgcc tgaagctgcg ggcattcccg atcagaaatg agcgccagtc
      361 gtcgtcggct ctcggcaccg aatgcgtatg attctccgcc agcatggctt cggccagtgc
      421 gtcgagcagc gcccgcttgt tcctgaagtg ccagtaaagc gccggctgct gaacccccaa
      481 ccgttccgcc agtttgcgtg tcgtcagacc gtctacgccg acctcgttca acaggtccag
      541 ggcggcacgg atcactgtat tcggctgcaa ctttgtcatg cttgacactt tatcactgat
      601 aaacataata tgtccaccaa cttatcagtg ataaagaatc cgcgcgttca atcggaccag
      661 cggaggctgg tccggaggcc agacgtgaaa cccaacagac ccctgatcgt aattctgagc
      721 actgtcgcgc tcgacgctgt cggcatcggc ctgattatgc cggtgctgcc gggcctcctg
      781 cgcgatctgg ttcactcgaa cgacgtcacc gcccactatg gcattctgct ggcgctgtat
      841 gcgttgatgc aatttgcctg cgcacctgtg ctgggcgcgc tgtcggatcg tttcgggcgg
      901 cggccggtct tgctcgtctc gctggccggc gctgctgtcg actacgccat catggcgacg
      961 gcgcctttcc tttgggttct ctatatcggg cggatcgtgg ccggcatcac cggggcgact
     1021 ggggcggtag ccggcgctta tattgccgat atcactgatg gcgatgagcg cgcgcggcac
     1081 ttcggcttca tgagcgcctg tttcgggttc gggatggtcg cgggacctgt gctcggtggg
     1141 ctgatgggcg gtttctcccc ccacgctccg ttcttcgccg cggcagcctt gaacggcctc
     1201 aatttcctga cgggctgttt ccttttgccg gagtcgcaca aaggcgaacg ccggccgtta
     1261 cgccgggagg ctctcaaccc gctcgcttcg ttccggtggg cccggggcat gaccgtcgtc
     1321 gccgccctga tggcggtctt cttcatcatg caacttgtcg gacaggtgcc ggccgcgctt
     1381 tgggtcattt tcggcgagga tcgctttcac tgggacgcga ccacgatcgg catttcgctt
     1441 gccgcatttg gcattctgca ttcactcgcc caggcaatga tcaccggccc tgtagccgcc
     1501 cggctcggcg aaaggcgggc actcatgctc ggaatgattg ccgacggcac aggctacatc
     1561 ctgcttgcct tcgcgacacg gggatggatg gcgttcccga tcatggtcct gcttgcttcg
     1621 ggtggcatcg gaatgccggc gctgcaagca atgttgtcca ggcaggtgga tgaggaacgt
     1681 caggggcagc tgcaaggctc actggcggcg ctcaccagcc tgacctcgat cgtcggaccc
     1741 ctcctcttca cggcgatcta tgcggcttct ataacaacgt ggaacgggtg ggcatggatt
     1801 gcaggcgctg ccctctactt gctctgcctg ccggcgctgc gtcgcgggct ttggagcggc
     1861 gcagggcaac gagccgatcg ctgatcgtgg aaacgatagg cctatgccat gcgggtcaag
     1921 gcgacttccg gcaagctata cgcgccctag gagtgcggtt ggaacgttgg cccagccaga
     1981 tactcccgat cacgagcagg acgccgatga tttgaagcgc actcagcgtc tgatccaaga
     2041 acaaccatcc tagcaacacg gcggtccccg ggctgagaaa gcccagtaag gaaacaactg
     2101 taggttcgag tcgcgagatc ccccggaacc aaaggaagta ggttaaaccc gctccgatca
     2161 ggccgagcca cgccaggccg agaacattgg ttcctgtagg catcgggatt ggcggatcaa
     2221 acactaaagc tactggaacg agcagaagtc ctccggccgc cagttgccag gcggtaaagg
     2281 tgagcagagg cacgggaggt tgccacttgc gggtcagcac ggttccgaac gccatggaaa
     2341 ccgcccccgc caggcccgct gcgacgccga caggatctag cgctgcgttt ggtgtcaaca
     2401 ccaacagcgc cacgcccgca gttccgcaaa tagcccccag gaccgccatc aatcgtatcg
     2461 ggctacctag cagagcggca gagatgaaca cgaccatcag cggctgcaca gcgcctaccg
     2521 tcgccgcgac cccgcccggc aggcggtaga ccgaaataaa caacaagctc cagaatagcg
     2581 aaatattaag tgcgccgagg atgaagatgc gcatccacca gattcccgtt ggaatctgtc
     2641 ggacgatcat cacgagcaat aaacccgccg gcaacgcccg cagcatcgcg accgtcatcg
     2701 gtgagaagtt cggcaggtat tgggtggtga caatgtaggt gctgccccaa atggcaggtg
     2761 ctatcgctgt gaacaataaa tcgggcgtgc gtaatgacat gcgattacaa gacctccgct
     2821 acggcgatgg gtttttgaga gttgcaggcg ccgcgatgac cgcaccctga cttggactgg
     2881 ccctgcctcg caccaatcca cctgcggcta tggccgcgat catgcgaggc ccgaaaatta
     2941 cacgacctgt acacgtccgc cgaacccgga acgcaacaga gcttcgtggg catcccgatc
     3001 cgggtcgtag cagcagtcct ggaccaaacg cacgtcgtag tccgcatcac tagcccaggc
     3061 gacgcttgaa agaacaacgc cggtggtgct tatcccggcc atgacaagcg tgcttacgcc
     3121 gcgcgtccga aggtcggcgt cgagcgccgt gccataaaag acgctggctc gcggacatgc
     3181 atagaaaagg tcgccccgtt cgatcgcaag cccttcgacg ggcagaccgg tgcgaaatcg
     3241 tccgctcggg agatacggtg agatttggcg attcgtcgcc ggcggcgcat gttcatactc
     3301 ttcgccgagc gaaaagttgg ggaacagcac gggccggccg cgcaaggcgt gctggcagcc
     3361 gtgcagaccc tgcgtgagat gaacgccgac aacctgcgca aggtgccggc cgatgcaccc
     3421 acggccttca tcaagccgcg ctggaagccg ctggtgatca ccccggaagg cctcgaccgg
     3481 aaattctacg aaatctgcgc cctgtccgag ctgaagaacg ccctgcgctc cggcgacatc
     3541 tgggtcaagg gctcgcggca gttccgcgac ttcgacgact acctgctgcc ggccgagaag
     3601 ttcgccgcac tcaagcgcga gcag
//
LOCUS       DAJVUJ010000407         1264 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.guided.316, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000407 DAJVUJ010000000
VERSION     DAJVUJ010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.guided.316"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            30..1163
                     /locus_tag="OQQ48_004409"
     CDS             30..1163
                     /locus_tag="OQQ48_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCS1954904.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQMPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
BASE COUNT          335 a          324 c          307 g          298 t
ORIGIN      
        1 cgggccgttt tgtatggaaa ccagacctta tgttcaaaac gacgctctgc gccttattaa
       61 ttaccgcctc ttgctccaca tttgctgccc ctcaacaaat caacgatatt gtgcatcgca
      121 caattacccc gcttatagag caacaaaaga tccccggtat ggcggtggcg gtaatttatc
      181 agggtaaacc ttattacttt acctggggct atgcggacat cgccaaaaag cagcccgtca
      241 cacagcaaac gttgtttgag ttaggttcgg tcagcaaaac atttacgggc gtgcttggtg
      301 gcgatgctat tgctcgaggg gaaatcaagt taagcgatcc cgcaacaaaa tactggcctg
      361 aacttaccgc taaacagtgg aatgggatca cactattaca tcttgcgacc tacaccgctg
      421 gcggcctgcc attgcagatg ccggatgaag tgaaatcctc aagcgacttg ctgcgcttct
      481 atcaaaactg gcagcctgca tgggctccag gaacacaacg tctgtatgcc aactccagta
      541 tcggtttgtt cggcgcactg gctgtgaagc cgtctggttt gagttttgag caggcgatgc
      601 aaactcgtgt cttccagcca ctcaaactca accatacgtg gattaatgta ccgcccgcag
      661 aagaaaagaa ttacgcctgg ggatatcgcg aaggcaaggc agttcatgtt tcgccagggg
      721 cgttagatgc tgaagcttat ggtgtgaagt cgaccattga agatatggcc cgctgggtgc
      781 aaagcaattt aacccccctt gatatcaatg agaaaacgct tcaacaaggg atacaactgg
      841 cacaatctcg ctactggcaa accggcgata tgtatcaggg cctgggctgg gaaatgctgg
      901 actggccggt caatcctgac agcatcatta acggcagtga caataaaatt gcactggcag
      961 cacgcccggt aaaagcgatt aagcccccaa ctcctgcagt acgcgcatca tgggtacata
     1021 aaacgggggc gaccggcgga tttggtagct atgtcgcgtt tattccagaa aaagagctgg
     1081 gtatcgtgat gctggctaac aaaaactatc ccaatccagc gagagtcgcc gccgcctgtc
     1141 agattctcaa cgctctacag taaaatttca tcgggtccga attttcggac cttttctgcg
     1201 cttttccttg ctgtcatcta cacttagaaa aaaaccagta aggaaacatt tatgcgcctg
     1261 ctcc
//
LOCUS       DAJVUJ010000408          760 bp    DNA     linear   BCT 11-NOV-2022
DEFINITION  TPA_asm: Shigella boydii isolate 300519
            SAMEA8899965-rid15856543.guided.353, whole genome shotgun sequence.
ACCESSION   DAJVUJ010000408 DAJVUJ010000000
VERSION     DAJVUJ010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899965
            Sequence Read Archive: ERR6005777
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella boydii
  ORGANISM  Shigella boydii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 10-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478691.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478691.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/11/2022 08:22:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,411
            CDSs (total)                      :: 4,327
            Genes (coding)                    :: 3,992
            CDSs (with protein)               :: 3,992
            Genes (RNA)                       :: 84
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 335
            CDSs (without protein)            :: 335
            Pseudo Genes (ambiguous residues) :: 0 of 335
            Pseudo Genes (frameshifted)       :: 187 of 335
            Pseudo Genes (incomplete)         :: 93 of 335
            Pseudo Genes (internal stop)      :: 96 of 335
            Pseudo Genes (multiple problems)  :: 38 of 335
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..760
                     /organism="Shigella boydii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899965-rid15856543.guided.353"
                     /serovar="S. boydii 2"
                     /isolate="300519"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:621"
                     /country="Mozambique"
                     /collection_date="2008-06-18"
     gene            6..464
                     /gene="gpt"
                     /locus_tag="OQQ48_004410"
     CDS             6..464
                     /gene="gpt"
                     /locus_tag="OQQ48_004410"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCS1954905.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            556..>760
                     /locus_tag="OQQ48_004411"
     CDS             556..>760
                     /locus_tag="OQQ48_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCS1954906.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILD"
BASE COUNT          178 a          197 c          192 g          193 t
ORIGIN      
        1 ttcacatgag cgaaaaatac atcgtcacct gggacatgtt gcagatccat gcacgtaaac
       61 tcgcaagccg actgatgcct tctgaacaat ggaaaggcat tattgccgta agccgtggcg
      121 gtctggtacc gggtgcgtta ctggcgcgtg aactgggtat tcgtcatgtc gataccgttt
      181 gtatttccag ctacgatcac gacaaccagc gagagcttaa agtgctgaaa cgcgcagaag
      241 gcgatggcga aggcttcata gttattgatg acctggtgga taccggtggt actgctgttg
      301 cgattcgtga aatgtatcca aaagcgcact ttgtcaccat cttcgcaaaa ccggctggtc
      361 gtccgctggt tgatgactat gttgttgata ttccgcaaga tacctggatc gaacagccgt
      421 gggatatggg cgtcgtattc gtcccgccaa tctccggtcg ctaatctttt caacgcctgg
      481 cactgccggg cgttgttctt tttaacttca ggcaggttac aatagtttcc agtaagtatt
      541 ctggaggctg catccatgac acaggcaaac ctgagcgaaa ccctgttcaa accccgcttt
      601 aaacatcctg aaacctcgac gctagtccgc cgctttaatc acggcgcaca accgcctgtg
      661 cagtcggctc ttgatggtaa aaccatccct cactggtatc gcatgattaa ccgtctgatg
      721 tggatctggc gcggtattga cccacgtgaa atcctcgacg
//
